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Sample records for blood group incompatibility

  1. Results of exchange transfusions in newborns without blood group incompatibility

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    Servet Yel

    2013-01-01

    Full Text Available Objective: Hyperbilirubinemia is a common problem ofneonatal period that has high morbidity and mortality.Blood exchange is the most effective and urgent treatmentmodality for very high bilirubin levels that can lead toneurotoxicity called as kernicterus. The aim of this studywas to compare 90 minutes exchange transfusion withthat of 120 minutes.Methods: This study was performed at Dicle UniversityMedical Faculty, Neonatal Unit between July 2007 andJune 2008. A total of 36 term newborn (38 - 42 gestationalweek without blood group incompatibility and withtotal serum bilirubin levels over 25 mg/dl were included.Newborns were randomly assigned in two groups eachof them comprise 18 babies as Group 1 underwent 90minute-exchange and Group 2 120 minute. Effectivenessand complications of exchange transfusion were recorded.Newborns with Rh, ABO or subgroup incompatibilities,prematurity or small for gestational age, septicemia,hypothyroidism, G6PD enzyme deficiency, intrauterineinfections, diabetic mother’s baby, hemolytic disease ormetabolic diseases were excluded.Results: There were no significant differences in thebody weight, gestational age, postnatal age, age of mother,total bilirubin and albumin levels, the number of bloodexchange, hospital stay days and complications betweentwo groups (p>0.05. However, mean phototherapy durationwas significantly shorter in 120 minutes transfusiongroup compared with 90 minutes group (p<0.001.Conclusion: Our results indicated that 90 minutes wassufficient for an effective exchange transfusion in severehyperbilirubinemic newborn infants. However longer exchangetransfusion durations may shorten the duration ofphototherapy.Key words: Indirect hyperbilirubinemia, exchange transfusion,newborns, outcome

  2. Case report: diffuse splenic metastasis of occult breast cancer with incompatible blood group antigenic determinants.

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    Baranyay, Ferenc

    2009-01-01

    Cancer cells with immunogenic properties having altered protein glycosilation, modified blood group substances have been widely studied [Kannagi R, Miyake M, Zenita KM, Itai S, Hiraiwa N, Shigeta K, et al. Cancer-associated carbohydrate antigens: modified blood group substances and oncodevelopmental antigens on tumor cells. Gann Monogr Cancer Res 1988; 34: p. 15-28; Hakomori S. Antigen structure and genetic basis of histo-blood groups A, B and O their changes associated with human cancer. Biochem Biophys Acta 1999; 1473: p. 247-266; Brooks SA, Carter TM, Royle L, Harvey DJ, Fry SA, Kinch C, et al. Altered glycosilation of proteins in cancer: what is the potential for new anti-tumour strategies. Anticancer Agents Med Chem 2008; 8: p. 2-21]. In the study reported here, a 78-year-old female patient was admitted to the hospital with circulatory failure. At autopsy, the spleen (weight: 420 g) was extremely firm with a diffusely blackberry-colored cut surface. There were no signs of carcinomatous process at autopsy. By histology, the spleen showed diffuse metastatic carcinomatous infiltration. Using immunohistochemistry, an antibody to breast carcinoma antigen (BioGenex) labelled metastatic cells of the spleen and bone marrow. The patient was blood group O. Labelling for binding of lectins with and without blood group antigen specificity and monoclonal antibodies was carried out. The B blood group specific Banderiaea simplicifolia agglutinin I and an anti-B blood group monoclonal antibody labelled all the metastatic cells of spleen and bone marrow intensely. There was no detection of blood group A antigen by either binding of Dolichos biflorus agglutinin or anti-blood group A monoclonal antibodies. These observations raise the possibility that the detected incompatible B blood group antigen determinants on the metastatic cells were immunogenic. The surviving carcinoma cells may have found a place of refuge from immune surveillance in the spleen and in the bone marrow

  3. Biosynthetic basis of incompatible histo-blood group A antigen expression

    DEFF Research Database (Denmark)

    David, L; Leitao, D; Sobrinho-Simoes, M;

    1993-01-01

    , we have screened 31 cases of gastric tumors of phenotype O for the expression of blood group A gene-defined glycosyltransferase by immunohistology on frozen sections using newly developed monoclonal antibodies to the transferases. Three cases were positive, and transferase expression was confirmed...... by enzyme analysis of extracts from the specimens. Blood group A carbohydrate antigens were also identified immunohistologically in these three cases as well as in five other cases. Thin-layer chromatography immunostaining analysis of glycolipid extracts from the three cases did not confirm the chemical...

  4. 王翠霞教授治疗母儿ABO血型不合经验%Professor WANG Cui-xia's Summary of Experience of Treating Fetomaternal ABO Blood Group Incompatibility

    Institute of Scientific and Technical Information of China (English)

    薛晓岚

    2012-01-01

    Fetomaternal blood group incompatibility is immune diseases of blood type because that pregnant women and the fetus haven't the same blood group, all of them fetomaternal ABO blood group incompatibility is usual, according to inspection of serum antibodies to diagnose, its incidence is high and dangerous, who attacks more and more persons' attention. My teacher professor WANG Cui-xia considered that the etiology is mainly the hot, humid implication, stagnation of qi and blood stasis, damp and hot resistance to quadruplet, blood stasis in Chong and Ren, fetus not solid, tai displaced and raise the cost of disease, and can the wet, puring the heat, promoting blood circulation to remove blood stasis, and invigorating kidney are the therapeutic principles, the clinical curative effect is distinct.%母儿血型不合是孕妇和胎儿之间因血型不合产生的同族血型免疫性疾病,其中以ABO血型不合常见,需依据检验血清抗体确诊,其发病率高且危害性大,越来越受到重视.吾师王翠霞教授认为母儿ABO血型不合的病因主要为湿热内蕴及气滞血瘀,湿热蕴阻胞胎,冲任瘀阻,胎元不固,胎失所养而成本病,并以清热利湿及活血化瘀佐以补肾安胎法为治疗原则,临床疗效显著.

  5. Red blood cell-incompatible allogeneic hematopoietic progenitor cell transplantation.

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    Rowley, S D; Donato, M L; Bhattacharyya, P

    2011-09-01

    Transplantation of hematopoietic progenitor cells from red cell-incompatible donors occurs in 30-50% of patients. Immediate and delayed hemolytic transfusion reactions are expected complications of red cell-disparate transplantation and both ABO and other red cell systems such as Kidd and rhesus can be involved. The immunohematological consequences of red cell-incompatible transplantation include delayed red blood cell recovery, pure red cell aplasia and delayed hemolysis from viable lymphocytes carried in the graft ('passenger lymphocytes'). The risks of these reactions, which may be abrupt in onset and fatal, are ameliorated by graft processing and proper blood component support. Red blood cell antigens are expressed on endothelial and epithelial tissues in the body and could serve to increase the risk of GvHD. Mouse models indicate that blood cell antigens may function as minor histocompatibility antigens affecting engraftment. Similar observations have been found in early studies of human transplantation for transfused recipients, although current conditioning and immunosuppressive regimens appear to overcome this affect. No deleterious effects from the use of red cell-incompatible hematopoietic grafts on transplant outcomes, such as granulocyte and platelet engraftments, the incidences of acute or chronic GvHD, relapse risk or OS, have been consistently demonstrated. Most studies, however, include limited number of patients, varying diagnoses and differing treatment regimens, complicating the detection of an effect of ABO-incompatible transplantation. Classification of patients by ABO phenotype ignoring the allelic differences of these antigens also may obscure the effect of red cell-incompatible transplantation on transplant outcomes. PMID:21897398

  6. Frecuencias de grupos sanguíneos e incompatibilidades ABO y RhD, en La Paz, Baja California Sur, México Blood group ABO and RhD frequencies and incompatibilities in La Paz, Baja California Sur, Mexico

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    Lorenzo del Peón-Hidalgo

    2002-09-01

    (Zone General Hospital of the Mexican Institute of Social Security in La Paz, Baja California Sur, Mexico. Blood donors were typified by tube agglutination. The gene frequencies were estimated assuming equilibrium conditions, and incompatibilities and alloimmunization were statistically assessed with the chi² test. Results. Percent frequencies were as follows: blood group O, 58.49%; A, 31.40%; B, 8.40%; AB, 1.71%; RhD, 95.36%; and RhD negative, 4.64%. Genic frequencies were: i, 0.7648; I A, 0.1821; I B, 0.0519; D, 0.7845; and d, 0.2155, respectively. Incompatibilities between couples and mother-child were 0.3023 and 0.1685 for ABO, 0.0442 and 0.0364 for RhD, and 0.0134 and 0.0061 for double incompatibility, respectively. The probability of maternal alloimmunization was estimated at 0.0309. Conclusions. The O and RhD groups were the most common in La Paz, although frequencies were among the lowest in Mexico, contrary to the case of A and RhD negative groups. The probabilities of maternal alloimmunization and of incompatibilities were also high. Ancestral white, black, and indigenous groups admixed in the northwestern part of Mexico; after migrating to Baja California Sur the admixture of the population probably became similar to that of the remainder of the northwestern area.

  7. Neonatal BO Incompatibility Is Associated With a Positive Cord Blood Direct Antiglobulin Test in Infants of Black Ethnicity.

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    Özgönenel, Bülent; Kukreja, Geetika; O'Malley, Barbara; Bluth, Martin H

    2015-11-01

    ABO hemolytic disease of the newborn occurs almost exclusively in infants of blood group A and B who are born to group O mothers. Positive Direct Antiglobulin Test (DAT) can identify those infants who are at risk of developing the ABO hemolytic disease. Earlier studies have suggested that BO incompatibility is associated with a positive DAT in black infants. In this study we sought to determine whether ABO incompatibility type could be associated with a higher rate of DAT positivity or clinical hemolytic disease. We reviewed the electronic medical records of all ABO-incompatible births over a 2-year period. There were 1537 ABO-incompatible births during the study period. DAT was more commonly positive among BO incompatible (21.5% in BO vs. 14.8% in AO, P=0.001) and black (18.8% in blacks vs. 10.8% in nonblacks, P=0.003) infants. DAT positivity was significantly associated with both severe hyperbilirubinemia (P=0.028) and hemolytic anemia (P<0.001). BO incompatibility was significantly associated with hemolytic anemia, but not severe hyperbilirubinemia, in the infants tested. PMID:26422285

  8. [Blood group typing in the cat].

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    Haarer, M; Grünbaum, E G

    1993-08-01

    Blood group serological diagnosis in cats is clinically relevant for the prophylaxis of blood group incompatibility reactions. In permanent blood donors, cats used for breeding and recipients with a history of prior blood transfusions, testing should consist of blood typing and antibody detection. As test sera and test cells are not commercially available and since parallel tests for various antibody qualities are necessary, they are usually performed in specialized laboratories. Incompatibility testing has a practical clinical relevance in finding a serological diagnosis before each blood transfusion and in cases of kitten mortality. In emergency situations, cross matching can be performed on slides as a screening test. Negative slide test results then should be verified using the more sensitive test tube or microtiter plate tests. PMID:8211961

  9. Pediatric patient with Bombay blood group: A rare case report

    OpenAIRE

    Sudeshna Bhar (Kundu); Anisha De; Anindita Saha; Chiranjib Bhattacharyya

    2015-01-01

    Bombay blood group is a rare blood group in which there is the absence of H antigen and presence of anti-H antibodies. At the time of blood grouping, this blood group mimics O blood group due to the absence of H antigen, but it shows incompatibility with O group blood during cross matching. Serum grouping or reverse grouping are essential for confirmation of the diagnosis. Patients carrying this blood group can receive blood only from a person with this blood group. Reported cases of anesthes...

  10. Blood groups systems

    OpenAIRE

    Ranadhir Mitra; Nitasha Mishra; Girija Prasad Rath

    2014-01-01

    International Society of Blood Transfusion has recently recognized 33 blood group systems. Apart from ABO and Rhesus system, many other types of antigens have been noticed on the red cell membranes. Blood grouping and cross-matching is one of the few important tests that the anaesthesiologist orders during perioperative period. Hence, a proper understanding of the blood group system, their clinical significance, typing and cross-matching tests, and current perspective are of paramount importa...

  11. Comparative genetic organization of incompatibility group P degradative plasmids.

    OpenAIRE

    Burlage, R S; Bemis, L A; Layton, A C; Sayler, G. S.; Larimer, F

    1990-01-01

    Plasmids that encode genes for the degradation of recalcitrant compounds are often examined only for characteristics of the degradative pathways and ignore regions that are necessary for plasmid replication, incompatibility, and conjugation. If these characteristics were known, then the mobility of the catabolic genes between species could be predicted and different catabolic pathways might be combined to alter substrate range. Two catabolic plasmids, pSS50 and pSS60, isolated from chlorobiph...

  12. Blood groups systems

    Directory of Open Access Journals (Sweden)

    Ranadhir Mitra

    2014-01-01

    Full Text Available International Society of Blood Transfusion has recently recognized 33 blood group systems. Apart from ABO and Rhesus system, many other types of antigens have been noticed on the red cell membranes. Blood grouping and cross-matching is one of the few important tests that the anaesthesiologist orders during perioperative period. Hence, a proper understanding of the blood group system, their clinical significance, typing and cross-matching tests, and current perspective are of paramount importance to prevent transfusion-related complications. Nonetheless, the knowledge on blood group system is necessary to approach blood group-linked diseases which are still at the stage of research. This review addresses all these aspects of the blood groups system.

  13. Blood groups systems.

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    Mitra, Ranadhir; Mishra, Nitasha; Rath, Girija Prasad

    2014-09-01

    International Society of Blood Transfusion has recently recognized 33 blood group systems. Apart from ABO and Rhesus system, many other types of antigens have been noticed on the red cell membranes. Blood grouping and cross-matching is one of the few important tests that the anaesthesiologist orders during perioperative period. Hence, a proper understanding of the blood group system, their clinical significance, typing and cross-matching tests, and current perspective are of paramount importance to prevent transfusion-related complications. Nonetheless, the knowledge on blood group system is necessary to approach blood group-linked diseases which are still at the stage of research. This review addresses all these aspects of the blood groups system. PMID:25535412

  14. Pediatric patient with Bombay blood group: A rare case report.

    Science.gov (United States)

    Bhar Kundu, Sudeshna; De, Anisha; Saha, Anindita; Bhattacharyya, Chiranjib

    2015-01-01

    Bombay blood group is a rare blood group in which there is the absence of H antigen and presence of anti-H antibodies. At the time of blood grouping, this blood group mimics O blood group due to the absence of H antigen, but it shows incompatibility with O group blood during cross matching. Serum grouping or reverse grouping are essential for confirmation of the diagnosis. Patients carrying this blood group can receive blood only from a person with this blood group. Reported cases of anesthesia in the pediatric patient with Bombay blood group are relatively rare. Here, we present successful anesthetic management along with intraoperative blood transfusion in a pediatric patient with Bombay blood group posted for ovarian cystectomy. PMID:26240554

  15. Pediatric patient with Bombay blood group: A rare case report

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    Sudeshna Bhar (Kundu

    2015-01-01

    Full Text Available Bombay blood group is a rare blood group in which there is the absence of H antigen and presence of anti-H antibodies. At the time of blood grouping, this blood group mimics O blood group due to the absence of H antigen, but it shows incompatibility with O group blood during cross matching. Serum grouping or reverse grouping are essential for confirmation of the diagnosis. Patients carrying this blood group can receive blood only from a person with this blood group. Reported cases of anesthesia in the pediatric patient with Bombay blood group are relatively rare. Here, we present successful anesthetic management along with intraoperative blood transfusion in a pediatric patient with Bombay blood group posted for ovarian cystectomy.

  16. Pediatric patient with Bombay blood group: A rare case report.

    Science.gov (United States)

    Bhar Kundu, Sudeshna; De, Anisha; Saha, Anindita; Bhattacharyya, Chiranjib

    2015-01-01

    Bombay blood group is a rare blood group in which there is the absence of H antigen and presence of anti-H antibodies. At the time of blood grouping, this blood group mimics O blood group due to the absence of H antigen, but it shows incompatibility with O group blood during cross matching. Serum grouping or reverse grouping are essential for confirmation of the diagnosis. Patients carrying this blood group can receive blood only from a person with this blood group. Reported cases of anesthesia in the pediatric patient with Bombay blood group are relatively rare. Here, we present successful anesthetic management along with intraoperative blood transfusion in a pediatric patient with Bombay blood group posted for ovarian cystectomy.

  17. Meta analysis of the effect of immunoglobulin infusion on neonatal isoimmune hemolytic disease caused by blood group incompatibility%新生儿血型不合溶血病丙种球蛋白治疗安全性和有效性的Meta分析

    Institute of Scientific and Technical Information of China (English)

    李志华; 王瑾; 陈超

    2010-01-01

    immunoglobulin infusion on neonatal isoimmune hemolytic disease by meta analysis.Method All randomized controlled trials on the effect of immunoglobulin infusion on neonatal Rh and ABO incompatible hemolytic disease obtained by searching MEDLINE,Cochrane Library,EMBASE,CNKI and CBM were included.Meta analysis was done by Review Manager 4.2software.Results Six trials with totally 456 neonates were included.There were 109 infants with Rh blood group incompatible hemolysis in 4 studies and 347 infants with ABO blood group incompatible hemolysis in 4 studies.There was no significant difference in gestational age,weight and sex between the immunoglobulin infusion and control groups.Compared with those neonates treated with only phototherapy,the infants treated with immunoglobulin and phototherapy had shorter duration of phototherapy(weighted mean difference,WMD - 15.42,95% CI - 29.00 to - 1.85),less chance to be given exchange transfusion(RR 0.25,95% GI 0.17 to 0.39)and shorter duration of hospitalization(WMD - 25.44,95% GI - 36.93 to- 13.94).While intravenous immunoglobulin could not decrease the maximum serum bilirubin level(WMD -29.91,95%CI -78.24 to 18.42).There was no significant difference in the incidence of late anemiabetween the two groups.No adverse reaction was found in neonates who received immunoglobulin.Conclusions The results of this meta analysis support that the intravenous immunoglobulin had some therapeutic effect on neonatal isoimmune hemolytic disease.The infants who received immunoglobulin had shorter duration of phototherapy and less chance to be given exchange transfusion.Well designed,double blind and randomized controlled trials with large sample size and long-term follow-up are needed for further evaluation of the efficacy and safety of the immunoglobulin therapy.

  18. Flowering time and incompatibility groups: Cultivar combination in commercial sweet cherry (Prunus avium L. orchards

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    Radičević Sanja

    2011-01-01

    Full Text Available The paper presents results of eight-year study (1999-2006 of flowering phenophase in 21 introduced sweet cherry cultivars grown under the agro-environmental conditions of West Serbia. Flowering time, as well as progress and abundance of flowering were studied, and classification of the studied cultivars according to flowering time was derived. On the basis of mean several-year overlap in phenophase of full flowering and on the grounds of so far known data on classification of these cultivars among incompatibility groups, we have offered a recommendation for their cultivation in orchards whereby the most effective pollination and fertilization can be ensured as well as good fruit-set and satisfactory fruit yields.

  19. Integration of noninvasive prenatal prediction of fetal blood group into clinical prenatal care

    DEFF Research Database (Denmark)

    Clausen, Frederik Banch

    2014-01-01

    Incompatibility of red blood cell blood group antigens between a pregnant woman and her fetus can cause maternal immunization and, consequently, hemolytic disease of the fetus and newborn. Noninvasive prenatal testing of cell-free fetal DNA can be used to assess the risk of hemolytic disease...

  20. Duffy Blood Group System and the malaria adaptation process in humans

    OpenAIRE

    Gledson Barbosa de Carvalho; Glauber Barbosa de Carvalho

    2011-01-01

    Malaria is an acute infectious disease caused by the protozoa of the genus Plasmodium. The antigens of the Duffy Blood Group System, in addition to incompatibilities in transfusions and hemolytic disease of the newborn, are of great interest in medicine due to their association with the invasion of red blood cells by the parasite Plasmodium vivax. For invasions to occur an interaction between the parasites and antigens of the Duffy Blood Group System is necessary. In Caucasians six antigens a...

  1. Transcription of ColE1Ap mbeC induced by conjugative plasmids from twelve different incompatibility groups.

    OpenAIRE

    Selvaratnam, S; Gealt, M A

    1993-01-01

    Although nonconjugative mobilizable plasmids require helping functions of conjugative plasmids in order to be mobilized into recipients, at least some genes from the nonconjugative plasmids may be induced to assist in the DNA transfer process. Conjugative plasmids from 12 different incompatibility groups mobilized the nonconjugative plasmid ColE1Ap between Escherichia coli strains. Introduction of any of the conjugative plasmids into the ColE1Ap-containing strain resulted in an induction of m...

  2. A case of nearly mistaken AB para-Bombay blood group donor transplanted to a group 'O' recipient.

    Science.gov (United States)

    Townamchai, Natavudh; Watanaboonyongcharoen, Phandee; Chancharoenthana, Wiwat; Avihingsanon, Yingyos

    2014-10-31

    Unintentional ABO mismatch kidney transplantation can cause detrimental hyperacute rejection. We report the first successful ABO incompatible kidney transplantation from an AB para-Bombay donor to O recipient. At the initial evaluation, the donor's ABO type was discordance on the cell typing and serum typing, which typed to be 'O' as cell typing and 'AB' as serum typing. At the second investigation, it was confirmed that the donor had a unique, rare but not uncommon blood type AB para-Bombay which was incompatible with the recipient's blood group. The kidney transplantation was successfully performed by an ABO incompatible preconditioning, double filtration plasmapheresis (DFPP) and rituximab. The serum creatinine at 12 months post-transplantation was 1.3 mg/dL. The pathology of the kidney biopsy showed no signs of rejection.

  3. The Indian blood group system.

    Science.gov (United States)

    Xu, Q

    2011-01-01

    The Indian blood group system (ISBT: IN/023) consists of two antithetical antigens: In(a) (IN1), which is present in approximately 10 percent of some Arab populations and in 3 percent of Bombay Indians, and its allelic antigen In(b) (IN2), an antigen of high incidence in all populations. In 2007, two new high-incidence antigens were identified as belonging to the IN blood group system, namely IN3 (INFI) and IN4 (INJA). The antigens in this system are located on CD44, a single-pass membrane glycoprotein that is encoded by the CD44 gene on chromosome 11 at position p13. The biologic function of CD44 is as a leukocyte homing receptor and cellular adhesion molecule. The In(a) and In(b) polymorphism represents a 252G>C substitution of CD44, encoding R46P, and lack of IN3 and IN4 results from homozygosity for mutations encoding H85Q and T163R in the CD44 gene. The high-frequency antigen AnWj (901009) has not been assigned to the Indian system, but either is located on an isoform of CD44 or is closely associated with it.

  4. Enterococcus faecalis hemolysin-bacteriocin plasmids belong to the same incompatibility group.

    OpenAIRE

    Colmar, I; Horaud, T

    1987-01-01

    Plasmid pair coexistence was studied both among nine Enterococcus faecalis hemolysin-bacteriocin (Hly-Bcn) plasmids, including pJH2, pAD1, pAM gamma 1, and pIP964, and between pIP964 and five R plasmids. Some of the Hly-Bcn plasmids used were derivatives encoding resistance to erythromycin or tetracycline. The Hly-Bcn plasmids were incompatible with each other; 40 to 100% displacement was observed bilaterally for eight pairs and unilaterally for one pair. In contrast, pIP964 stably coexisted ...

  5. Relationship between blood groups and male infertility

    International Nuclear Information System (INIS)

    Background: Blood is man's complete and unchangeable identity. The ABO and Rh groups are recognised as major and clinically significant blood groups. Blood group antigens are not only important in relation to blood transfusion and organ transplantation, but also have been utilised in genetic research, anthropology and tracing ancestral relation of humans. The objective the present study is to examine the blood group antigens in infertile men for assessing the relationship to male infertility and to know the frequency of various blood groups among infertile males in our population. Method: A total of 1,521 patients along with 460 proven fathers as controls were recruited for the present study from both rural and urban areas of Pakistan and referred to Department of Reproductive Physiology/Health, Public Health Divisions, NIH, Islamabad, during 2002 to 2006. Blood grouping (ABO) and Rhesus factors (Rh) was done by the antigen antibody agglutination test. Results: Overall distribution of blood groups in the studied population of 1,521 subjects was 35.50%, 28.27%, 26.89% and 9.34% for blood groups O, B, A and AB respectively. The ratio of control to patient was 1:3.3. Conclusions: The present preliminary study revealed that in our population the prevalence of male infertility in blood group O is invariably higher than in all other ABO blood groups, showing a strong relationship between blood group O and male infertility. (author)

  6. ASSOCIATION BETWEEN GLAUCOMA AND BLOOD GROUPS

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    F. Ghannadi R. Varmazyar

    2006-09-01

    Full Text Available There are reports from different countries that some types of glaucoma are associated with blood groups. This cross-sectional study was performed on 400 glaucomatous patients [100 patients in each group of Primary open angle glaucoma (POAG, chronic angle closure glaucoma (CACG, pseudoexfoliative glaucoma (PEXG and primary congenital glaucoma (PCG] and 400 blood donors as control group to assess the association between blood groups and glaucoma. All patients underwent ABO and Rh blood group testing. The prevalence of blood group A was 30% in the control group, 27% in POAG, 33% in CACA, 38% in PEXG and 36% in PCG. The prevalence of blood group B was 24% in the control group, 19% in POAG, 20% in CACG, 15% in PEXG and 34% in PCG (P < 0.025. The prevalence of blood group AB was 8% in the control group, 9% in POAG, 5% in CACG, 12% in PEXG, and 8% in PCG. The prevalence of blood group O was 38% in the control group, 45% in POAC, 42% in CACG, 35% in PEXG and 22% in PCG (P < 0.001. The prevalence of Rh+ was 88% in the control group, 84% in POAG, 87% in CACG, 86% in PEXG and 87% in PCG. Compared to control group, blood group B was more prevalent and blood group O was less prevalent in PCG. There was no association between other types of blood groups (ABO and Rh and PCG. There was no association between blood groups (ABO and Rh and other types of glaucoma.

  7. ABO blood groups and susceptibility to brucellosis.

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    Mohsenpour, Behzad; Hajibagheri, Katayon; Afrasiabian, Shahla; Ghaderi, Ebrahim; Ghasembegloo, Saeideh

    2015-01-01

    The relationship between blood groups and some infections such as norovirus, cholera, and malaria has been reported. Despite the importance of brucellosis, there is a lack of data on the relationship between blood groups and brucellosis. Thus, in this study, we examined the relationship between blood groups and brucellosis. In this case-control study, the blood groups of 100 patients with brucellosis and 200 healthy individuals were studied. Exclusion criteria for the control group consisted of a positive Coombs Wright test or a history of brucellosis. The chi-square test was used to compare qualitative variables between the two groups. The variables that met inclusion criteria for the regression model were entered into the logistic regression model. A total of 43% patients were female and 57% male; 27% were urban and 73% rural. Regression analysis showed that the likelihood of brucellosis infection was 6.26 times more in people with blood group AB than in those with blood group O (Pbrucellosis infection. Thus, there is a relationship between blood group and brucellosis. People with blood group AB were susceptible to brucellosis, but no difference was observed for brucellosis infection in terms of blood Rh type.

  8. Relationship between ABO blood groups and malaria*

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    Gupta, Madhu; Chowdhuri, A. N. Rai

    1980-01-01

    A total of 736 patients with fever was tested for malaria and classified according to ABO blood group. Of these, 476 cases had patent parasitaemia at the time of investigation. The distribution of blood groups in this group was significantly different from that in 1300 controls from the same area. While group A was found to be more common in malaria cases than in normals, the reverse situation was found for group O. Possible explanations for this are discussed. PMID:6971187

  9. Research on two specimens policy with blood transfusion management system in prevention incompatible blood%二次血型制配合输血管理系统阻止不相容血液发放的研究

    Institute of Scientific and Technical Information of China (English)

    王远杰; 刘家瑞; 李红梅; 冯宁; 冯俊程; 杨晓丽

    2015-01-01

    次血型鉴定错误的血样中,如果根据这些血液样本的血型鉴定结果发放血液,其中3份可导致急性溶血性输血反应,存在输血安全风险(25%,3/12).采用2次血型制,每年可避免约1次血型不相容输血.血袋因采、供血机构已进行多次血型检测、复核,其安全风险无法用具体数据评估.结论 2次血型检测制配合输血管理系统能防止不相容血液的发放,大幅减低输血安全风险.%Objective To investigate the role and significance of two specimens policy cooperated with blood transfusion management system in the prevention of incompatible blood.Methods From March 2011 to June 2014,a total of 120 962 blood samples from outpatients and inpatients in Suining Central Hospital and 24 963 blood bags (suspended red blood cells) were collected into this study,as research objects.Inclusion criteria:no damage and leakage,no exception to the naked eye;Exclusion criteria:pollution.The study protocol was approved by the Ethical Review Board of Investigation in Human at Suining Central Hospital.Patients' blood samples were carried out twice independent blood group appraisals,respectively,as the initial blood grouping and second blood grouping.At the same time,the blood group appraisal and the irregular antibody screening of the blood bags were carried out.All data input into the blood transfusion management system,and the blood transfusion management system automatically compared these entry data.If twice blood group appraisal results were consistent,and irregular antibody screening results were negative,the blood can be extended.Otherwise,blood transfusion management system could prevent to extend blood,and issue a warning.Twice blood grouping error rates were computed and corrected.Blood samples of which twice blood group appraisals cannot match were tested again,to distinguish between initial sample error and second sample error.Blood transfusion safety risks were computed.Blood bags of which

  10. ABO blood group and risk of cancer

    DEFF Research Database (Denmark)

    Vasan, Senthil K; Hwang, Jinseub; Rostgaard, Klaus;

    2016-01-01

    INTRODUCTION: The associations between ABO blood group and cancer risk have been studied repeatedly, but results have been variable. Consistent associations have only been reported for pancreatic and gastric cancers. MATERIALS AND METHODS: We estimated associations between different ABO blood gro...... tract (mouth, salivary glands, pharynx, esophageal adenocarcinoma and stomach). DISCUSSION: Our study reconfirms the association between ABO blood group and cancer risk and exact underlying mechanisms involved needs further research....

  11. Chinese medical researches of maternal-fetal ABO blood type incompatibility%母儿ABO血型不合的中医药研究进展

    Institute of Scientific and Technical Information of China (English)

    胡凤英; 赵蕾; 吕玲

    2010-01-01

    母罂ABO血型不合可导致多种新生儿疾病甚至死亡,部分核黄疸幸存儿的智力发育多受到影响.本文综述了母婴ABO血型不合的病因病机、孕前期和妊娠期中医药治疗的方法及其疗效.%Maternal-fetal ABO blood type incompatibility is an autoimmune disease.This disease can lead to miscarriage,stillbirth,children edema,early-onset neonatal jaundice,hemolytic anemia,kemicterus and neonatal death,etc.The author overviewed the etiology and pathogenesis of Matemal-fetal ABO blood type incompatibility,as well as the TCM therapeutic methods and effects in treating this disease at pre-pregnancy period and pregnancy.

  12. [Blood groups - minuses and pluses. Do the blood group antigens protect us from infectious diseases?].

    Science.gov (United States)

    Czerwiński, Marcin

    2015-06-25

    Human blood can be divided into groups, which is a method of blood classification based on the presence or absence of inherited erythrocyte surface antigens that can elicit immune response. According to the International Society of Blood Transfusion, there are 341 blood group antigens collected in 35 blood group systems. These antigens can be proteins, glycoproteins or glycosphingolipids, and function as transmembrane transporters, ion channels, adhesion molecules or receptors for other proteins. The majority of blood group antigens is present also on another types of cells. Due to their localization on the surface of cells, blood group antigens can act as receptors for various pathogens or their toxins, such as protozoa (malaria parasites), bacteria (Helicobacter pylori, Vibrio cholerae and Shigella dysenteriae) and viruses (Noroviruses, Parvoviruses, HIV). If the presence of group antigen (or its variant which arised due to mutation) is beneficial for the host (e.g. because pathogens are not able to bind to the cells), the blood group may become a selection trait, leading to its dissemination in the population exposed to that pathogen. There are thirteen blood group systems that can be related to pathogen resistance, and it seems that the particular influence was elicit by malaria parasites. It is generally thought that the high incidence of blood groups such as O in the Amazon region, Fy(a-b-) in Africa and Ge(-) in Papua-New Guinea is the result of selective pressure from malaria parasite. This review summarizes the data about relationship between blood groups and resistance to pathogens.

  13. Importance of blood groups and blood group antibodies in companion animals.

    Science.gov (United States)

    Hohenhaus, Ann E

    2004-04-01

    Dogs, cats, birds, and ferrets are popular companion animals. Because these pets are considered by many to be family members, they are provided high-quality veterinary medical care, including blood transfusions. This article reviews the current status of blood groups in dogs, cats, birds, and ferrets and discusses the impact of blood groups on veterinary transfusion medicine. One blood group with 3 types has been described in the cat, whereas multiple blood groups have been described in the dog. Only rudimentary knowledge exists regarding pet bird blood groups, and, to date, the ferret appears to be unique because no blood groups have been described. Antibodies against blood group antigens also play a role in animal blood transfusions. Cats have naturally occurring alloantibodies; however, dogs do not appear to have clinically significant naturally occurring alloantibodies. Understanding the issues related to blood groups and blood group antibodies in companion animals will also benefit those using these species as research models for human diseases. PMID:15067591

  14. Late antibody-mediated rejection after ABO-incompatible kidney transplantation during Gram-negative sepsis

    NARCIS (Netherlands)

    A. de Weerd (Annelies); A.G. Vonk (Alieke); H. van der Hoek (Hans); M. van Groningen (Marian); W. Weimar (Willem); M.G.H. Betjes (Michiel); M. Agteren (Madelon)

    2014-01-01

    textabstractBackground: The major challenge in ABO-incompatible transplantation is to minimize antibody-mediated rejection. Effective reduction of the anti-ABO blood group antibodies at the time of transplantation has made ABO-incompatible kidney transplantation a growing practice in our hospital an

  15. Blood Groups in the Kashmir Valley

    Directory of Open Access Journals (Sweden)

    Rafiq A.Calcutti, Mohammed Khali Lone, Showket Ahmed,Bashir A.Shah, Neelofer Jan.

    2003-07-01

    Full Text Available Blood groups are genetically determined and exJ1ibit polymorphism, where different populationgroups have significant difference in the frequency of each blood group. This study wasconducted to determine the frequency of ABO and Rhesus D blood groups among the blooddO:lors. A total number of 1306 blood donors attended the donor centre at SKIMS MedicalCollege Hospital for blood donation in the year 2001-02. After each donation blood sampleswere collected in separate pilot hlbes for the estimation of ABO and Rhesus D blood groups.The frequency of O. A, Band AB, Rhesus D positive and Rhesus D negative were calculatedseparately. The highest li"equency among the ABO blood groups was ofB (39.43% and the lowestwas of AB (8.11 %. Among the Rhesus D phenotypes. majority (93.33% were RhesusD positive. where as only 6.67% were Rhesus D negative. The prevalence of ABO/Rhesus D wascalculated and the highest frequency was o1'B Rh-D positive (37.44% followed by a Rh-D positive(28.9-+%. A Rh-D positive (19.21 %, AB Rh-D positive (7.73%, a Rh-D negative (2.90%,B Rh-D negati'c (1.99%, A Rh-D negative (1.37% and AB Rh-D negative (0.38%. Thisstudy showed that most common group was B followed by a & A and 93.33% were positive forRh-D phenotype.

  16. Blood group antigen distribution in Lao blood donors.

    Science.gov (United States)

    Keokhamphoui, C; Urwijitaroon, Y; Kongphaly, D; Thammavong, T

    2012-01-01

    Blood group antigens can be distributed differently within different nationalities. Therefore, information about the prevalence of blood group antigens in the Lao population will be useful for providing better blood transfusion services in the Lao People's Democratic Republic. The purpose of this study was to determine the prevalence of blood group antigens in Lao blood donors. Blood samples from 464 Lao national volunteer blood donors were typed for antigens in various blood group systems including ABO, MNS, P1PK, Rh, Kell, Lewis, Duffy, Kidd, and Diego. The results show similar antigen prevalence to that among Northeast Thais for ABO, MNS, P1PK, Rh, Kell, and Duffy systems. In the ABO system, 0 was the highest at 37.72 percent,followed by 35.56 percent B, 19.83 percent A1, 6.47 percent A1B,and 0.43 percent A2B. The common phenotypes were D+C+E-ce+at 60.43 percent, M+N-S-s+ at 46.55 percent, Fy(a+b-) at 80.82 percent, Jk(a+b+) at 39.44 percent, and kk at 99.72 percent.Interestingly, Le(a-b-) was found at 50.43 percent, which was significantly higher than previous reports in Thais and Taiwanese.The P1 antigen was found in only 18.97 percent, which is much lower than in Whites and Blacks. Rare phenotypes were Fy(a-b+)and Jk(a-b-), found at 0.22 percent and 4.31 percent, respectively.In terms of negative antigens the study shows 0.22 percent Fy(a-), 35.34 percent Jk(a-), 29.53 percent Jk(b-), 3.04 percent C-, 2.39 percent e-, and 5.17 percent M-. The high prevalence of C, e, and Fy" and immunogenicity of these antigens may induce alloimmunization in transfusion-dependent patients, creating difficulties providing blood from Lao donors. The information obtained from this study will be useful for improving transfusion therapy in the country, especially for estimation of the availability of compatible blood for patients who have produced antibodies. PMID:23421543

  17. [Solid phase techniques in blood group serology].

    Science.gov (United States)

    Uthemann, H; Sturmfels, L; Lenhard, V

    1993-06-01

    As alternatives to hemagglutination, solid-phase red blood cell adherence assays are of increasing importance. The adaptation of the new techniques to microplates offers several advantages over hemagglutination. Using microplates the assays may be processed semiautomatically, and the results can be read spectrophotometrically and interpreted by a personal computer. In this paper, different red blood cell adherence assays for AB0 grouping, Rh typing, Rh phenotyping, antibody screening and identification, as well as crossmatching will be described.

  18. Lectins as markers for blood grouping.

    Science.gov (United States)

    Khan, Fauzia; Khan, Rizwan H; Sherwani, Asma; Mohmood, Sameena; Azfer, Md A

    2002-12-01

    Lectins are unique proteins of varying biological importance. They are characterized by specific binding to carbohydrate residues, whether monosaccharides, disaccharides or polysaccharides. The sugar heads on the surface of the erythrocyte specify the different blood groups. Lectins, as an antigenic determinant of blood group, have come to be an important tool in the identification of different blood groups. A handful of lectins may be considered excellent reagents for anti-A, anti-B, anti-N etc, but the anti-A and anti-M are not yet regarded as commercially suitable antisera. Lectin from Vicia cracca has been proved to be a good anti-A, lectin from Dolichus biflorus can be used as anti-A1, and lectin from Griffonia simplicifolia as anti-B. Lectin from Vicia graminea is said to be a good typing reagent as Anti-N. On the other hand, the lectins involved in polyagglutination are absolutely essential as the reagent of choice and these cannot as yet be replaced by antibodies of any kind. Erythrocytes with exposed cryptantigens are significantly more sensitive to agglutination by certain lectins than by polyclonal antibodies. Peanut agglutinin (PNA), Polybrene, and Glycine max lectins are frequently used for the identification of different cryptantigens. The application of lectins as an anti-B reagent has proven to be as useful as human polyclonal or mouse monoclonal antibodies. Besides their specificity, lectins are excellent reagents because of their lower cost and indigenous production. The importance of various lectins used as markers for blood grouping is discussed.

  19. ABO incompatibility hemolytic disease following exchange transfusion 96 newborn

    Directory of Open Access Journals (Sweden)

    Khatami S.F

    2007-09-01

    Full Text Available Background: ABO incompatibility hemolytic disease of the newborn is a common cause of clinical jaundice and causes two-thirds of the hemolytic disease in newborns. This study was undertaken to determine the frequency of ABO incompatibility hemolytic disease and its complications in newborns undergoing exchange transfusion.Methods: This prospective and descriptive study was performed in jaundiced newborn infants during a three-year period. Inclusion criteria were: maternal blood type O, newborn blood type A or B, rising indirect hyperbilirubinemia in the first two days of life, positive immunohematologic test for newborns and exchange transfusion. Exclusion criteria were: incomplete information, other accompanying diseases that induce hyperbilirubinemia. All newborn infants received phototherapy before and after exchange transfusion. We did not use intravenous immunoglobulin, hemoxygenase inhibitor drugs and blood products before exchange transfusion.Results: Double-volume exchange transfusion via umbilical cord catheter was performed in 96 patients, 19 (20% of whom suffered from ABO incompatibility. Of these 19 newborns, two-thirds (13 were preterm infants. The minimum level of serum bilirubin was 10 mg/dl and the maximum serum bilirubin level was 35 mg/dl. In six patients (32% serum bilirubin levels were >25mg/dl. The most common blood group was type A for newborns. Immunohematologic tests were positive in 84% of the mothers. ABO incompatibility hemolytic disease was the fourth and second most common reasons for blood exchange transfusion in preterm and term infants, respectively. Laboratory complications were more common than clinical complications. The etiology of 48% of the alloimmunization and 42% of the hemolytic disease in these newborns was ABO incompatibility.Conclusions: Mothers with blood group O and newborns with blood group A or B with positive immunohematologic tests in first hours of life are at high risk for hemolytic disease

  20. Association of Rotavirus Gastroenteritis with Histo-blood Group Antigens.

    Science.gov (United States)

    Mohanty, E; Dwibedi, B; Kar, S K; Pandey, R M

    2016-07-01

    Association of rotavirus gastroenteritis with histo-blood group antigens in children younger than 5 years admitted with diarrhea (n=389) was studied. Distribution of blood groups in rotavirus positive (n=96) and rotavirus negative (n=51) diarrhea gastroenteritis cases did not show any susceptibility to any blood group; blood group O seemed to be protective. PMID:27508550

  1. Qualitative analysis fingertip patterns in ABO blood group

    Directory of Open Access Journals (Sweden)

    S. V. KShirsagar

    2013-05-01

    Full Text Available The inheritance of the dermatoglyphic patterns is polygenic. The genetic basis of the blood group is well established. The correlation between the dermatoglyphic patterns and the ABO blood group is studied by some workers in different populations. In the present study, the correlation between dermatoglyphics and ABO blood group is studied in the Marathwada Region of Maharashtra. The qualitative data included fingertip patterns and three indices. It was observed that, the Arch pattern is more common in blood group O both in male and female. Ulnar loop is most common in the blood group AB. Simple whorl and double loop whorl patterns are less frequent in blood group AB. Accidentals were not recorded in blood group A while blood group O show highest percentage of accidentals. Dankmeijer’s index was highest in blood group AB and lowest in blood group B.

  2. DERMATOGLYPHICS AND BLOOD GROUPS: A REVIEW

    Directory of Open Access Journals (Sweden)

    Abha

    2015-06-01

    Full Text Available Finger Print is known to be the best tool of identification. The term dermatoglyphic was coined by Harold Cummin in 1926 and was classified by Sir Francis Galton into loops , whorls and arches. Since then finger prints have been used for determining physical and mental health. Various studies have been carried out throughout the globe to prove its association with Down’s syndrome , Schizophrenia , Rubel la embryopathy , other genetic disorders and gender variations. Studies also suggest a correlation of finger prints and blood groups. Hence an effort was made to review the literature on this aspect.

  3. Kidd blood group system: a review.

    Science.gov (United States)

    Hamilton, Janis R

    2015-01-01

    The Kidd blood group system has been recognized as clinically important in red blood cell (RBC) serology since its identification in 1951. Forty years later, the JK glycoprotein was determined to be a product of SCL14A1 and was identical to the urea transport protein UT-B produced by HUT11A. The functional role of the protein as a urea transporter in RBC and kidney has been well documented. The polymorphism responsible for the antithetical anigens Jk(a) and Jk(b) was identified in 1994 as c.838G>A (p.Asp280Asn). Recent discoveries have expanded the system to include 23 variant alleles recognized by the International Society of Blood Transfusion that silence the protein expression and 7 variant alleles presumably producting weak or partia JK antigens. Null phenotypes have been identified in individuals of several populations including those of African, Indian, and Chinese decent, in addition to the well-documented findings in the Polynesian and Finnish populations. This review will examine the historical information about the anigens and antibodies of the JK system as well as catalog the variations of the JK gene. PMID:26308468

  4. Relationship between ABO blood groups and oral cancer

    Directory of Open Access Journals (Sweden)

    Bushranaaz Fathima Jaleel

    2012-01-01

    Conclusion: By employing a simple blood grouping test during community field programs, people with blood group A in the age group of 40-59 years having tobacco chewing habits can be apprised that they are more at risk to develop oral cancer than people with other blood groups.

  5. A questionnaire on survival of kittens depending on the blood groups of the parents.

    Science.gov (United States)

    Axnér, Eva

    2014-10-01

    Cats more than 2 months of age have alloantibodies against the blood type antigen that they do not possess. Maternal antibodies, including alloantibodies against blood groups, are transferred to the kittens' systemic circulation when they suckle colostrum during the first 12-16 h after birth. If kittens with blood group A or AB nurse from a mother with blood group B they may develop neonatal isoerythrolysis (NI). Breeders can prevent kittens at risk of NI from nursing during the first 16-24 h; after this period it is safe to let them nurse. Kittens depend, however, on the passive transfer of antibodies from the colostrum for early protection against infections. Although it is known that kittens deprived of colostrum will also be deprived of passive systemic immunity, it is not known if this will affect their health. Therefore, the aim of this study was to evaluate kitten mortality in litters with B-mothers and A-fathers compared to litters with A-mothers. In addition, the aim was to evaluate the effects of colostrum deprivation on the health of the mothers, and the breeders' opinions and experiences of these combinations of breedings. A web-based questionnaire was constructed and distributed to breeders. The results indicate that there is no difference in mortality between planned litters that have mothers with blood group A and litters with mothers that have blood group B and fathers that have blood group A. When managing blood group incompatibility in cat all factors affecting the health of the cats, including genetic variation, should be considered. PMID:24423812

  6. [ABO BLOOD GROUPS AS RISK FACTOR IN HELICOBACTER PYLORI INFECTION

    Science.gov (United States)

    Gonzáles Flores, Pedro Alejandro; Díaz Ferrer, Javier Omar; Monge Salgado, Eduardo; Watanabe Varas T, Teresa

    2000-01-01

    TITLE: ABO blood groups as risk factor in Helicobacter pylori infection.OBJECTIVE: To asses the relation between ABO blood groups and Helicobacter pylori (Hp) infection. METHODS: The present is a case and control study. A study population of dyspeptic patients who underwent upper gastrointestinal endoscopy was selected. Four biopsies were taken from the antrum and the body of the stomach and blood group was typified. Patients with gastrectomy, gastric cancer, treated for Hp infection in the previous six months or without blood group typification were excluded. The population sample was found using EPIINFO 5.1 program. We called case to every patient with Hp (+) biopsy and control all with Hp (-) biopsy. The risk of the infection was calculated with the OR (Odds ratio) and the study sample was compared with the blood bank control group using the Chi-square test (pblood groups between the study population and the blood bank control. When we compared the ABO blood distribution between patients Hp (+) and Hp (-) we found significant differences for blood group O (p=0.004) and blood group A (p=0.03). Statistical analysis revealed an OR=2,22 for the blood group O and OR=0,5 for the blood group A.CONCLUSIONS: 1) The ABO blood group distribution is different in patients with Hp infection compared with those without Hp infection. 2) Blood group O would be a moderate risk factor for infection by Helicobacter pylori. PMID:12140571

  7. Genetic basis of rare blood group variants

    NARCIS (Netherlands)

    L. Wigman

    2013-01-01

    A transfusion of donor red blood cells can be life saving In individuals with massive blood loss due to an accident or surgery or in individuals with constitutive anemia due to a defect in erythropoiesis. Donor blood can, however, not be simply transfused to every patient. When a recipient of a red

  8. LOW RATE OF RHESUS IMMUNIZATION FROM RH-INCOMPATIBLE BLOOD TRANSFUSIONS DURING LIVER AND HEART TRANSPLANT SURGERY

    OpenAIRE

    Ramsey, Glenn; Hahn, Linda F.; Cornell, Frank W.; Boczkowski, David J.; Staschak, Sandee; Clark, Roxann; Hardesty, Robert L.; Griffith, Bartley P.; Starzl, Thomas E.

    1989-01-01

    Transfusion of one unit or more of Rh-positive red blood cells normally causes circulating anti-D antibody to appear 2–6 months later in 80–95% of Rh− persons. We asked whether transplant immunosuppression with cyclosporine and corticosteroids affects Rh immunization. Nineteen Rh− liver, heart, and heart-lung transplant recipients received 3–153 (median: 10) units of Rh+ RBCs at surgery and were tested for anti-D >2 months later. Three patients developed anti-D at 11–15 days; one may have had...

  9. Identification of the XG blood group gene

    Energy Technology Data Exchange (ETDEWEB)

    Ellis, N.A.; Reid, M.; German, J. [New York Blood Center, NY (United States)] [and others

    1994-09-01

    A candidate for the XG blood group gene, called PBDX, was isolated that spans the pseudoautosomal boundary on the X chromosome. Using rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of the predicted mature PBDX, we have identified the Xg{sup a} antigen encoded by the XG gene: in indirect hemagglutination assays, the anti-peptide antibodies react with Xg(a+) but not with Xg(a-) cells. By antibody-specific immobilization of antigen (ASIA) and Western blot assays, the anti-peptide antibodies react with the same molecule as that with which human anti-Xg{sup a} reacts. By its identity with PBDX, therefore, Xg{sup a} is recognized as a cell-surface antigen 48% homologous to CD99 (previously referred to as the 12E7 antigen) encoded in the tightly linked locus MIC2. Taken together with previous findings, the evidence strongly suggests that the XG polymorphism is defined by a difference in the level of Xg{sup a} present on the surface of the erythrocyte rather than a difference in the amino acid sequences of the protein products encoded in the Xg{sup a} and the so-far silent Xg alleles.

  10. Identification and Characterization of Peptide Mimics of Blood Group A Antigen

    Institute of Scientific and Technical Information of China (English)

    Zhaoming TANG; Lin WANG; Lihua HU; Yirong LI; Tianpen CUI; Juan XIONG; Lifang DOU

    2008-01-01

    In order to investigate peptide mimics of carbohydrate blood group A antigen, a phage display 12-met peptide library was screened with a monoclonal antibody against blood group A antigen, NaM87-1F6. The antibody-binding properties of the selected phage peptides were evaluated by phage ELISA and phage capture assay. The peptides were co-expressed as glutathione S-transferase (GST) fusion proteins. RBC agglutination inhibition assay was performed to assess the natural blood group A antigen-mimicking ability of the fusion proteins. The results showed that seven phage clones selected bound to NaM87-1F6 specifically, among which, 6 clones bore the same peptide sequence, EYWYCGMNRTGC and another harbored a different one QIWYERTLPFrF. The two peptides were successfully expressed at the N terminal of GST protein. Both of the fusion proteins inhibited the RBC agglutination mediated by anti-A serum in a concentration-dependent manner. These results suggested that the fusion proteins based on the selected peptides could mimic the blood group A an- tigen and might be used as anti-A antibody-adsorbing materials when immunoabsorption was applied in ABO incompatible transplantation.

  11. FREQUENCY OF BLOOD GROUPS AND TITERS OF ALLOANTIBODIES IN DOMESTIC CATS

    OpenAIRE

    Anderson Barros Teixeira Pinto; Miguel Angelo da Silva Medeiros; Mariana Palha de Brito Jardim; Antonio Peixoto Albernaz

    2016-01-01

    Hemotherapy requires reliable blood compatibility tests, such as blood typing, to avoid possible transfusion reactions in cats. However, it is also important to avoid neonatal isoerythrolysis. When blood transfusions are performed between incompatible feline donors and recipients, they may develop acute transfusion reactions, especially severe when type A blood is transfused into a type B cat because it has high levels of anti-A alloantibodies. Therefore, knowing on the blood type frequency i...

  12. Maximally incompatible quantum observables

    Energy Technology Data Exchange (ETDEWEB)

    Heinosaari, Teiko, E-mail: teiko.heinosaari@utu.fi [Turku Centre for Quantum Physics, Department of Physics and Astronomy, University of Turku, FI-20014 Turku (Finland); Schultz, Jussi, E-mail: jussi.schultz@gmail.com [Dipartimento di Matematica, Politecnico di Milano, Piazza Leonardo da Vinci 32, I-20133 Milano (Italy); Toigo, Alessandro, E-mail: alessandro.toigo@polimi.it [Dipartimento di Matematica, Politecnico di Milano, Piazza Leonardo da Vinci 32, I-20133 Milano (Italy); Istituto Nazionale di Fisica Nucleare, Sezione di Milano, Via Celoria 16, I-20133 Milano (Italy); Ziman, Mario, E-mail: ziman@savba.sk [RCQI, Institute of Physics, Slovak Academy of Sciences, Dúbravská cesta 9, 84511 Bratislava (Slovakia); Faculty of Informatics, Masaryk University, Botanická 68a, 60200 Brno (Czech Republic)

    2014-05-01

    The existence of maximally incompatible quantum observables in the sense of a minimal joint measurability region is investigated. Employing the universal quantum cloning device it is argued that only infinite dimensional quantum systems can accommodate maximal incompatibility. It is then shown that two of the most common pairs of complementary observables (position and momentum; number and phase) are maximally incompatible.

  13. The relationship between maternal blood group and preeclampsia

    Directory of Open Access Journals (Sweden)

    Manjunatha S.

    2015-12-01

    Conclusions: The present study indicates that AB blood group have the highest risk of developing preeclampsia. AB blood group is associated with an increased risk of thrombotic events this may be the cause of increased incidence of PIH in this group. Thus attention should be given to the AB blood group pregnant women in order to prevent the PIH. [Int J Reprod Contracept Obstet Gynecol 2015; 4(6.000: 1749-1752

  14. Significant association between ABO blood group and pancreatic cancer

    Institute of Scientific and Technical Information of China (English)

    Julia; B; Greer; Mark; H; Yazer; Jay; S; Raval; M; Michael; Barmada; Randall; E; Brand; David; C; Whitcomb

    2010-01-01

    AIM:To evaluate whether the ABO blood group is related to pancreatic cancer risk in the general population of the United States.METHODS:Using the University of Pittsburgh's clinicalpancreatic cancer registry,the blood donor database from our local blood bank (Central Blood Bank),and the blood product recipient database from the regional transfusion service (Centralized Transfusion Service) in Pittsburgh,Pennsylvania,we identified 274 pancreatic cancer patients with previously determined serological ABO bloo...

  15. Association of ABO blood groups with diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Narazah Mohd Yusoff

    2010-02-01

    Full Text Available Objective: So far no studies have been performed in Malaysia to look at association of diabetes mellitus (DM with blood groups. We studied the association of ABO blood groups with DM type 2. Patients and methodology: It was a case control study conducted at Kepala Batas Hospital Batas, Penang, Malaysia in the year 2009, involving 70 patients with DM type 2 and 140 healthy controls. Ethical approval was obtained from Universiti Sains Malaysia. Blood samples were collected from the patients after consent. Samples were tested for ABO blood groups using ID-Card gel method. Results: Chi-square test results showed that there was an association between the ABO blood groups and DM type 2. It was found that A and O blood groups were negatively associated with DM type 2 (P<0.05 with higher percentage of A and O groups individuals were non-diabetic. No significant association was noted between DM type 2 and blood groups B (P=0.423 and AB (P=0.095. It was also noted that B blood group was distributed with highest percentage among patients with DM type 2 (53.71% compared to controls (22.52%, but no statistical significance achieved. Conclusion: The results obtained suggest that there was a negative association between ABO blood groups A and O with DM type 2, with A and O group having less chances of diabetes. Large studies in other ethnic groups are needed to confirm these results.

  16. Case analysis of incompatibility in blood crossmatching induced by anti-Dia and anti-E antibodies%抗Dia合并抗E抗体致交叉配血不合的临床分析

    Institute of Scientific and Technical Information of China (English)

    刘永霞

    2015-01-01

    目的:探讨交叉配血中抗 Dia 合并抗 E 抗体致配血不合的原因。方法对该院1例因抗 Dia 合并抗 E 抗体致多次配血不合的患者进行分析。通过血型鉴定、直接抗人球蛋白试验、不规则抗体筛查、吸收放散实验来确定患者的血型、可能的不规则抗体、细胞谱反应格局。结果患者血型正定型:抗 A 抗体(-)、抗 B 抗体(-);反定型:Ac(++++)、Bc(++++)、RBC (-)、Oc(-);Rh 血型定型:抗 c 抗体(-)、抗 C 抗体(++++)、抗 D 抗体(++++)、抗 e 抗体(++++)、抗 E 抗体(-)。直接抗人球蛋白试验(-)。患者血清中含有抗 E、抗 c 抗体。结论患者血清中的不规则抗体是导致交叉配血不合的重要原因。%Objective To investigate the reason of anti-Dia combined with anti-E antibodies inducing incompatibility in blood crossmatching.Methods Retrospectively analyse a case of patient treated in the hospital,whose blood crossmatching failed for sev-eral times.Through blood typing,direct Coombs test,irregular antibody screening,adsorption and elution experiments to determine the patient′s blood type,possible irregular antibodies,cell spectral response pattern.Results Forward blood typing:anti-A antibody (-),anti-B antibodies(-);Reverse blood typing:Ac (++++),Bc(++++),RBC(-),Oc(-);Rh blood grouping:anti-c an-tibody(-),anti-C antibody (+ + + +),anti-D antibody (+ + + +),anti-e antibody (+ + + +),anti-E antibody (-),direct Coombs test(-).Patient′s serum contained anti-E and anti-c antibodies.Conclusion Irregular antibodies in the serum of patients is an important cause of crossmatch discord.

  17. Antibody to histo-blood group A antigen neutralizes HIV produced by lymphocytes from blood group A donors but not from blood group B or O donors

    DEFF Research Database (Denmark)

    Arendrup, M; Hansen, J E; Clausen, H;

    1991-01-01

    not inhibit the HTLV-IIIB/lyB or the HTLV-IIIB/lyO isolate. Specificity of the MAb-mediated inhibition was shown using A-antigen (tetrasaccharide). Thus, HIV infection of PBMC from donors with blood type A appears to induce expression of host-cell-encoded carbohydrate blood group A epitope on HIV which can...

  18. Para-Bombay phenotype: report of a rare blood group

    OpenAIRE

    A.Yashovardhan; I.S.Chaitanya Kumar; K.V. Sreedhar Babu; B. Suresh Babu; Anju Verma; Jothi Bai, D.S.; B. Siddhartha Kumar

    2012-01-01

    The blood sample of a 54-year-old male patient who presented with signs and symptoms suggestive of anaemia was submitted to the Blood Bank for blood grouping and cross-matching. In forward grouping, no agglutination was observed with A, B and AB antisera, but agglutination was noticed with D antiserum (Group O). In reverse grouping, there was agglutination in tube labelled A and no agglutination in tubes B and O (Group B) resulting in discrepancy between forward and reverse grouping. Furth...

  19. Flexible automated platform for blood group genotyping on DNA microarrays.

    Science.gov (United States)

    Paris, Sandra; Rigal, Dominique; Barlet, Valérie; Verdier, Martine; Coudurier, Nicole; Bailly, Pascal; Brès, Jean-Charles

    2014-05-01

    The poor suitability of standard hemagglutination-based assay techniques for large-scale automated screening of red blood cell antigens severely limits the ability of blood banks to supply extensively phenotype-matched blood. With better understanding of the molecular basis of blood antigens, it is now possible to predict blood group phenotype by identifying single-nucleotide polymorphisms in genomic DNA. Development of DNA-typing assays for antigen screening in blood donation qualification laboratories promises to enable blood banks to provide optimally matched donations. We have designed an automated genotyping system using 96-well DNA microarrays for blood donation screening and a first panel of eight single-nucleotide polymorphisms to identify 16 alleles in four blood group systems (KEL, KIDD, DUFFY, and MNS). Our aim was to evaluate this system on 960 blood donor samples with known phenotype. Study data revealed a high concordance rate (99.92%; 95% CI, 99.77%-99.97%) between predicted and serologic phenotypes. These findings demonstrate that our assay using a simple protocol allows accurate, relatively low-cost phenotype prediction at the DNA level. This system could easily be configured with other blood group markers for identification of donors with rare blood types or blood units for IH panels or antigens from other systems. PMID:24726279

  20. The Bombay blood group: are we out of risk?

    Science.gov (United States)

    Dipta, T F; Hossain, A Z

    2011-07-01

    The Bombay blood group is a rare blood group, phenotypes of this group lacking H antigen on the red cell membrane and have anti-H in the serum. It fails to express any A, B or H antigen on their red cells or other tissues. The existence of a human H/h genetic polymorphism was first established by Bhende et al. As first discovery in Bombay (Mumbai), in India in 1952, so the name of this rare blood group is known as Bombay blood group. People having Bombay phenotype are mostly confined to the Southeast Asia. Around 179 persons in India with a frequency of 1 in 10,000 have "Bombay Blood group". A high level of consanguinity present among the parents of the Bombay phenotype. The classic Bombay phenotype has been reported in those of Indian descendent. It is quite rare in Caucasian with an incidence of 1 in 250,000. As because in our country there is routine practice of "only forward or cell type grouping" using finger prick method by voluntary blood donors organization and various blood banks; so there is tremendous chance of misinterpretation or unexploration of this Bombay blood group. When misdiagnosed, this Bombay group can cause fatal haemolytic transfusion reaction. For this reason our suggestion is to incorporate "routine serum typing or reverse grouping confirmation" along with 'O' cell control in reverse grouping procedure in every Transfusion Medicine Department or Blood Bank or Blood Donor Centers and this practice should be mandatory to reduce the risk of fatal haemolytic transfusion reaction. In this view we will highlight the incidence, molecular biology and clinical significance of this rare and fatal blood group.

  1. Lea blood group antigen on human platelets

    International Nuclear Information System (INIS)

    One- and two-stage radioligand assays were used to determine if human platelets possess the Lea antigen. Goat IgG anti-Lea antibody was purified by multiple adsorptions with Le(a-b-) human red blood cells, followed by affinity chromatography with synthetic Lea substance and labeling with 125I. Human IgG anti-Lea antibody was used either in a two stage radioassay with 125I-labeled mouse monoclonal IgG anti-human IgG as the second antibody or, alternatively, purified by Staph protein A chromatography, labeled with 125I, and used in a one-stage radioassay. Platelets from donors of appropriate red blood cell phenotypes were incubated with the antisera, centrifuged through phthalate esters, and assayed in a gamma scintillation counter. Dose response and saturation curve analysis demonstrate the presence of Lewis a antigen on platelets from Lea+ donors. Furthermore, platelets from an Le(a-b-) donor incubated in Le (a+b-) plasma adsorb Lea antigen in a similar manner to red blood cells. The clinical significance of these antigens in platelet transfusion remains undefined

  2. Tissue distribution of histo-blood group antigens

    DEFF Research Database (Denmark)

    Ravn, V; Dabelsteen, Erik

    2000-01-01

    The introduction of immunohistochemical techniques and monoclonal antibodies to specific carbohydrate epitopes has made it possible to study in detail the tissue distribution of histo-blood group antigens and related carbohydrate structures. The present paper summarizes the available data...... concerning the histological distribution of histo-blood group antigens and their precursor structures in normal human tissues. Studies performed have concentrated on carbohydrate antigens related to the ABO, Lewis, and TTn blood group systems, i.e. histo-blood group antigens carried by type 1, 2, and 3 chain...... carrier carbohydrate chains. Histo-blood group antigens are found in most epithelial tissues. Meanwhile, several factors influence the type, the amount, and the histological distribution of histoblood group antigens, i.e. the ABO, Lewis, and saliva-secretor type of the individual, and the cell- and tissue...

  3. ABO Blood Group and Risk of Thromboembolic and Arterial Disease

    DEFF Research Database (Denmark)

    Vasan, Senthil K; Rostgaard, Klaus; Majeed, Ammar;

    2016-01-01

    BACKGROUND: ABO blood groups have been shown to be associated with increased risks of venous thromboembolic and arterial disease. However, the reported magnitude of this association is inconsistent and is based on evidence from small-scale studies. METHODS AND RESULTS: We used the SCANDAT2...... (Scandinavian Donations and Transfusions) database of blood donors linked with other nationwide health data registers to investigate the association between ABO blood groups and the incidence of first and recurrent venous thromboembolic and arterial events. Blood donors in Denmark and Sweden between 1987......-up. Compared with blood group O, non-O blood groups were associated with higher incidence of both venous and arterial thromboembolic events. The highest rate ratios were observed for pregnancy-related venous thromboembolism (incidence rate ratio, 2.22; 95% confidence interval, 1.77-2.79), deep vein thrombosis...

  4. Duffy blood group system and the malaria adaptation process in humans

    Directory of Open Access Journals (Sweden)

    Gledson Barbosa de Carvalho

    2011-02-01

    Full Text Available Malaria is an acute infectious disease caused by the protozoa of the genus Plasmodium. The antigens of the Duffy Blood Group System, in addition to incompatibilities in transfusions and hemolytic disease of the newborn, are of great interest in medicine due to their association with the invasion of red blood cells by the parasite Plasmodium vivax. For invasions to occur an interaction between the parasites and antigens of the Duffy Blood Group System is necessary. In Caucasians six antigens are produced by the Duffy locus (Fya, Fyb, F3, F4, F5 and F6. It has been observed that Fy(a-b- individuals are resistant to Plasmodium knowlesi and P. vivax infection, because the invasion requires at least one of these antigens. The P. vivax Duffy Binding Protein (PvDBP is functionally important in the invasion process of these parasites in Duffy / DARC positive humans. The proteins or fractions may be considered, therefore, an important and potential inoculum to be used in immunization against malaria.

  5. Phage type conversion in Salmonella enterica serotype Enteritidis caused by the introduction of a resistance plasmid of incompatibility group X (IncX)

    DEFF Research Database (Denmark)

    Brown, D. J.; Baggesen, Dorte Lau; Platt, D. J.;

    1999-01-01

    The plasmid pOG670, a 54 kb, conjugative plasmid that specifies resistance to ampicillin and kanamycin and belonging to the incompatibility group X (IncX), was transferred into 10 isolates of Salmonella enterica serotype Enteritidis belonging to 10 different phage types (PT1, 2, 3, 4, 8, 9, 9b, 10......, 11 and 13). Acquisition of the plasmid by these strains did not result in the loss of any resident plasmids but resulted in phage type conversion in 8 of the 10 strains (PT1, 2, 4, 8, 9, 9b, 10 and 11). The observed changes in phage type were found to result from the loss of sensitivity to 3...... of the 10 typing phages used (phages 3, 5 and 7). Where the conversion resulted in a change to a defined phage type, both the new and original PTs belonged to the same, previously described, evolutionary lines. Enteritidis PTs 1, 4 and 8, commonly associated with poultry world-wide, were converted to PTs 21...

  6. Quantitative blood group typing using surface plasmon resonance.

    Science.gov (United States)

    Then, Whui Lyn; Aguilar, Marie-Isabel; Garnier, Gil

    2015-11-15

    The accurate and reliable typing of blood groups is essential prior to blood transfusion. While current blood typing methods are well established, results are subjective and heavily reliant on analysis by trained personnel. Techniques for quantifying blood group antibody-antigen interactions are also very limited. Many biosensing systems rely on surface plasmon resonance (SPR) detection to quantify biomolecular interactions. While SPR has been widely used for characterizing antibody-antigen interactions, measuring antibody interactions with whole cells is significantly less common. Previous studies utilized SPR for blood group antigen detection, however, showed poor regeneration causing loss of functionality after a single use. In this study, a fully regenerable, multi-functional platform for quantitative blood group typing via SPR detection is achieved by immobilizing anti-human IgG antibody to the sensor surface, which binds to the Fc region of human IgG antibodies. The surface becomes an interchangeable platform capable of quantifying the blood group interactions between red blood cells (RBCs) and IgG antibodies. As with indirect antiglobulin tests (IAT), which use IgG antibodies for detection, IgG antibodies are initially incubated with RBCs. This facilitates binding to the immobilized monolayer and allows for quantitative blood group detection. Using the D-antigen as an example, a clear distinction between positive (>500 RU) and negative (anti-D IgG. Complete regeneration of the anti-human IgG surface is also successful, showing negligible degradation of the surface after more than 100 regenerations. This novel approach is validated with human-sourced whole blood samples to demonstrate an interesting alternative for quantitative blood grouping using SPR analysis. PMID:26047997

  7. Blood pressure, ethnic group, and salt intake in Belize.

    OpenAIRE

    Simmons, D

    1983-01-01

    A total of 1316 individuals were studied in seven villages in Belize, Central America. This represented 92% of the area population aged over 18. Generally, they were members of three ethnic groups--Maya, Spanish, and Creole. The systolic and diastolic IV and V blood pressures were recorded using standardised procedure. Significant differences in blood pressure, weight, and obesity were found between ethnic groups in both sexes, Creoles having higher means than the other groups. Significant re...

  8. Incompatibility and Mental Fatigue

    Science.gov (United States)

    Herzog, Thomas R.; Hayes, Lauren J.; Applin, Rebecca C.; Weatherly, Anna M.

    2011-01-01

    A straightforward prediction from attention restoration theory is that the level of incompatibility in a person's life should be positively correlated with that person's level of mental (or directed attention) fatigue. The authors tested this prediction by developing a new self-report measure of incompatibility in which they attempted to isolate…

  9. Correlation between 'H' blood group antigen and Plasmodium falciparum invasion.

    Science.gov (United States)

    Pathak, Vrushali; Colah, Roshan; Ghosh, Kanjaksha

    2016-06-01

    The ABO blood group system is the most important blood group system in clinical practice. The relationship between Plasmodium falciparum and ABO blood groups has been studied for many years. This study was undertaken to investigate the abilities of different blood group erythrocytes to support in vitro growth of P. falciparum parasites. P. falciparum parasites of four different strains (3D7, 7G8, Dd2 and RKL9) were co-cultured with erythrocytes of blood group 'A', 'B', 'O' (n = 10 for each) and 'O(h)' (Bombay group) (n = 7) for 5 days. Statistically significant differences were observed on the fourth day among the mean percent parasitemias of 'O', non-'O' ('A' and 'B') and 'O(h)' group cultures. The parasitemias of four strains ranged from 12.23 to 14.66, 11.68 to 13.24, 16.89 to 22.3, and 7.37 to 11.27 % in 'A', 'B', 'O' and Bombay group cultures, respectively. As the expression of H antigen decreased from 'O' blood group to 'A' and 'B' and then to Bombay blood group, parasite invasion (percent parasitemia) also decreased significantly (p group erythrocytes were virtually converted to Bombay group-like erythrocytes by the treatment of anti-H lectins extracted from Ulex europaeus seeds. Mean percent parasitemia of lectin-treated cultures on the fourth day was significantly lower (p Bombay group erythrocyte cultures, thus further strengthening the hypothesis.

  10. Para-Bombay phenotype: report of a rare blood group

    Directory of Open Access Journals (Sweden)

    A. Yashovardhan

    2012-07-01

    Full Text Available The blood sample of a 54-year-old male patient who presented with signs and symptoms suggestive of anaemia was submitted to the Blood Bank for blood grouping and cross-matching. In forward grouping, no agglutination was observed with A, B and AB antisera, but agglutination was noticed with D antiserum (Group O. In reverse grouping, there was agglutination in tube labelled A and no agglutination in tubes B and O (Group B resulting in discrepancy between forward and reverse grouping. Further testing confirmed that the individual's blood group was Para-Bombay B (Para-BH, which is a rare entity. The Para-Bombay phenotype is very rare. Only a few cases of Para-Bombay were reported in India till now and none from Andhra Pradesh. This entity is characterized by the absence of H, A and B antigens on the red cells but their presence in saliva and secretions of gastrointestinal and genitourinary tracts. Proper identification of this phenotype is very important; otherwise this particular blood group may be mislabelled as group O.

  11. Association of ABO and Rh Blood Groups to Blood-Borne Infections among Blood Donors in Tehran-Iran.

    OpenAIRE

    Fatemeh Mohammadali; Aliakbar Pourfathollah

    2014-01-01

    Abstract Background The aim of this study was to investigate the prevalence of hepatitis B, hepatitis C, HIV and syphilis infections in blood donors referred to Tehran Blood Transfusion Center (TBTC), and determine any association between blood groups and blood- borne infections between the years of 2005 and 2011. Methods This was a retrospective study conducted at TBTC. All of the donor serum samples were screened for HBV, HCV, HIV and syphilis by using third generation ELISA kits and RPR te...

  12. Cheiloscopy and blood groups: Aid in forensic identification

    OpenAIRE

    Bushra Karim; Devanand Gupta

    2014-01-01

    Introduction: Every person has certain features that make them radically distinct from others. One such feature is lip prints. Lip prints remain the same throughout life and are uninfluenced by injuries, diseases, or environmental changes. Different individuals have specific blood groups according to the various antigen–antibody reactions in their bloodstream. Aim: To determine the distribution of different patterns of lip prints among subjects having different ABO and Rh blood groups. ...

  13. Duffy blood group antigens: structure, serological properties and function

    OpenAIRE

    Ewa Łukasik; Kazimiera Waśniowska

    2016-01-01

    Duffy (Fy) blood group antigens are located on seven-transmembrane glycoprotein expressed on erythrocytes and endothelial cells, which acts as atypical chemokine receptor (ACKR1) and malarial receptor. The biological role of the Duffy glycoprotein has not been explained yet. It is suggested that Duffy protein modulate the intensity of the inflammatory response. The Duffy blood group system consists of two major antigens, Fya and Fyb, encoded by two codominant alleles designated FY*A and FY*B ...

  14. Antibody to histo-blood group A antigen neutralizes HIV produced by lymphocytes from blood group A donors but not from blood group B or O donors

    DEFF Research Database (Denmark)

    Arendrup, M; Hansen, J E; Clausen, H;

    1991-01-01

    for virus neutralization by the monoclonal antibody (MAb) AH16 directed against the blood group A epitope. MAb AH16 was previously shown to inhibit cell-free virus infection using HTLV-IIIB propagated in H9 cells. AH16 showed a concentration-dependent inhibition of the HTLV-IIIB/lyA isolate but did...... not inhibit the HTLV-IIIB/lyB or the HTLV-IIIB/lyO isolate. Specificity of the MAb-mediated inhibition was shown using A-antigen (tetrasaccharide). Thus, HIV infection of PBMC from donors with blood type A appears to induce expression of host-cell-encoded carbohydrate blood group A epitope on HIV which can......Three virus isolates HTLV-IIIB/lyA, HTLV-IIIB/lyB and HTLV-IIIB/lyO, obtained by passaging and propagating the HTLV-IIIB/H9 isolate in three separate cultures of mixed peripheral blood mononuclear cells (PBMC) from donors of blood type A, B or O, respectively, were tested for susceptibility...

  15. Human RBCs blood group conversion from A to O using a novel α-N-acetylgalactosaminidase of high specific activity

    Institute of Scientific and Technical Information of China (English)

    YU ChengYu; XU Hua; WANG LiSheng; ZHANG JianGeng; ZHANG YangPei

    2008-01-01

    α-N-acetylgalactosaminidase (aNAGA) can convert group A human red blood cells (RBCs) to group O. One novel aNAGA gene was cloned by PCR from Elizabethkingia meningosepticum isolated from a domestic clinical sample. Pure recombinant aNAGA was obtained by genetic engineering and protein purification with a calculated molecule of 49.6 kD. aNAGA was selective for terminal a-N-acetylgalacto-samine residue with a high specific activity, aNAGA could completely remove A antigens of 1 U (about 100 mL) group A1 or A2 RBCs in 1 h at pH 6.8 and 25℃ with a consumption of 1.5 or 0.4 mg recombinant enzyme. Enzyme-converted group A RBCs did not agglutinate after being mixed with monoclonal anti-A or sera of groups A, B, AB and O. Other blood group antigens except ABO had no change. FCM analy-sis showed that A antigens and A1 antigens disappeared while H antigens increased. It indicated thataNAGA successfully converted human blood group A RBCs to universally transfusable group O RBCs without the risk of ABO-incompatible transfusion reactions. This aNAGA was suitable for producing universal RBCs to increase clinical transfusion safety, improve the RBCs supply, and to decrease transfusion cost and support transfusion service in case of emergency,

  16. Integration of noninvasive prenatal prediction of fetal blood group into clinical prenatal care.

    Science.gov (United States)

    Clausen, Frederik Banch

    2014-05-01

    Incompatibility of red blood cell blood group antigens between a pregnant woman and her fetus can cause maternal immunization and, consequently, hemolytic disease of the fetus and newborn. Noninvasive prenatal testing of cell-free fetal DNA can be used to assess the risk of hemolytic disease of the fetus and newborn to fetuses of immunized women. Prediction of the fetal RhD type has been very successful and is now integrated into clinical practice to assist in the management of the pregnancies of RhD immunized women. In addition, noninvasive prediction of the fetal RhD type can be applied to guide targeted prenatal prophylaxis, thus avoiding unnecessary exposure to anti-D in pregnant women. The analytical aspect of noninvasive fetal RHD typing is very robust and accurate, and its routine utilization has demonstrated high sensitivities for fetal RHD detection. A high compliance with administering anti-D is essential for obtaining a clinical effect. Noninvasive fetal typing of RHC/c, RHE/e, and KEL may become more widely used in the future. PMID:24431264

  17. Distribution of ABO blood group in children with acute leukemias

    Directory of Open Access Journals (Sweden)

    Meliha Sakić

    2012-12-01

    Full Text Available Introduction: This study is the fi rst study about the distribution ABO blood types at children with acute leukemia in Federation of Bosnia and Herzegovina. The aim of the study is to point out distribution of blood type groups at children with acute leukemia (ALMethods: The number of children in this study was the following: 145 children with acute leukemia and 27 of children with acute myeloblastic leukemia (AML. All of the children were treated at Hemato- Oncology Unitof Pediatric Clinic in Sarajevo, in the period January 2000 until December 2010. Age of children was between 1 month and 15 years.Results: The results showed that different blood types were registered in 93. 1% of children who got ill and treated from acute leukemia for the mentioned period. At 6. 9 % of children, none of the blood types wereregistered. It was noticed that 40.9 % children who have registered blood type O, 37% blood type A,16% blood type B and 6.5% blood type AB had AL, too. It has been observed that children with following bloodtypes had AML: O, 47.8%, A, 47.7% and AB, 30.4%.Conclusion: Signifi cance ABO types distribution was confi rmed for children with ALL, p<0, 05. The analysis of the distribution of ABO types based on gender showed that signifi cance was confi rmed at females with both ALL and AML (p<0.05.

  18. Red blood cell phenotype matching for various ethnic groups.

    Science.gov (United States)

    Badjie, Karafa S W; Tauscher, Craig D; van Buskirk, Camille M; Wong, Clare; Jenkins, Sarah M; Smith, Carin Y; Stubbs, James R

    2011-01-01

    Patients requiring chronic transfusion support are at risk of alloimmunization after red blood cell (RBC) transfusion because of a disparity between donor and recipient antigen profiles. This research explored the probability of obtaining an exact extended phenotype match between blood donors randomly selected from our institution and patients randomly selected from particular ethnic groups. Blood samples from 1,000 blood donors tested by molecular method were evaluated for the predicted phenotype distribution of Rh, Kell, Kidd, Duffy, and MNS. A random subsample of 800 donor phenotypes was then evaluated for the probability of obtaining an exact match with respect to phenotype with a randomly selected patient from a particular ethnic group. Overall, there was a greater than 80 percent probability of finding an exact donor-recipient match for the K/k alleles in the Kell system. The probability ranged from 3 percent to 38 percent, depending on the ethnicity and disparities in phenotypic profiles, for the Rh, Kidd, Duffy, and MNS systems. A significant donor-recipient phenotype mismatch ratio exists with certain blood group antigens such that, with current routine ABO and D matching practices, recipients of certain ethnic groups are predisposed to alloimmunization. PMID:22356481

  19. Association of ABO and Rh Blood Groups to Blood-Borne Infections among Blood Donors in Tehran-Iran.

    Directory of Open Access Journals (Sweden)

    Fatemeh Mohammadali

    2014-07-01

    Full Text Available The aim of this study was to investigate the prevalence of hepatitis B, hepatitis C, HIV and syphilis infections in blood donors referred to Tehran Blood Transfusion Center (TBTC, and determine any association between blood groups and blood- borne infections between the years of 2005 and 2011.This was a retrospective study conducted at TBTC. All of the donor serum samples were screened for HBV, HCV, HIV and syphilis by using third generation ELISA kits and RPR test. Initial reactive samples were tested in duplicate. Confirmatory tests were performed on all repeatedly reactive donations. Blood group was determined by forward and reverse blood grouping. The results were subjected to chi square analysis for determination of statistical difference between the values among different categories according to SPSS program.Overall, 2031451 donor serum samples were collected in 2005-2011. Totally, 10451 were positive test for HBV, HCV, HIV and syphilis. The overall seroprevalence of HBV, HCV, HIV, and syphilis was 0.39%, 0.11%, 0.005%, and 0.010%, respectively. Hepatitis B and HIV infections were significantly associated with blood group of donors (P 0.05.Compared with neighboring countries and the international standards, prevalence of blood-borne infections is relatively low.

  20. Current molecular blood group technology:availability and practical applications

    Institute of Scientific and Technical Information of China (English)

    Willy A.Flegel

    2010-01-01

    @@ Almost all clinically important RBC antigens are defined at the molecular level.The expression of protein-and sugar-based antigens on the RBC surface can be predicted by determining the blood group gene variants(alleles).Most of the time,a single nucleotide polymorphism(sNP)distinguishes the allele,which determines an antigen and hence allows predicting the antigen.PCR with sequence specific priming(PCR-SSP)followed by gel electrophoresis was the original technique widely applied for blood group genotyping.Realtime PCR obviated the need for gels.

  1. [ABO system blood group ratios in patients with neuroinfections].

    Science.gov (United States)

    Rudometov, Iu P; Umanskiĭ, K G; Ashmarina, E E; Andreeva, L S

    1981-01-01

    The distribution of the ABO blood groups in 2009 patients including 1441 ones suffering from etiologically diverse neuroinfections was studied. Certain correlations between the nosological forms and groups of the diseases on the one hand, and the blood factors on the other are demonstrated. The data obtained point to a certain role of hereditary predisposition in the genesis of the neuroinfections. This predisposition predetermines the risk of the illnesses and the gravity of their course, the fact, which is of a practical importance for the clinician.

  2. Erythrina lectins detect the H/HI blood groups.

    Science.gov (United States)

    Sudakevitz, D; Gilboa-Garber, N; Levene, C; Sela, R; Bhattacharyya, L

    1991-08-01

    The lectin purified from Erythrina corallodendron seeds which binds N-acetyllactosamine greater than N-acetyl-D-galactosamine greater than alpha and beta galactosides greater than D-galactose was examined for its ABO(H) blood group specificity. It has been shown that this lectin causes the strongest hemagglutination of O(H) and weakest of Oh(Bombay) red blood cells, and interacts with the H antigen in association with the I antigen. The reactions of Erythrina corallodendron and Erythrina indica lectins (which are similar in sugar specificity) with erythrocytes of different ABO(H) and Ii blood groups (the I bloods were all from adults and the i from either cord or adult bloods) revealed the following order of activity: O(H)I greater than A2 I greater than O(H)i adult greater than A2BI greater than BI greater than O(H)i cord greater than A1I greater than A1i adult greater than Bi cord greater than A1BI greater than Ai cord greater than ABi cord greater than OhI. The Erythrina indica lectin showed a lower differentiation between the agglutination of O(H) and Oh erythrocytes. Both Erythrina lectins exhibited H/HI blood group preference but were not inhibited by the saliva from ABO(H) "secretors". Thus they may be classified with the Cytisus sessilifolius, Lotus tetragonolobus and Laburnum alpinum lectins which are inhibited by lactose but not by H blood group substances in secretions.

  3. Genetic characterization of the ABO blood group in Neandertals

    Directory of Open Access Journals (Sweden)

    Bertranpetit Jaume

    2008-12-01

    Full Text Available Abstract Background The high polymorphism rate in the human ABO blood group gene seems to be related to susceptibility to different pathogens. It has been estimated that all genetic variation underlying the human ABO alleles appeared along the human lineage, after the divergence from the chimpanzee lineage. A paleogenetic analysis of the ABO blood group gene in Neandertals allows us to directly test for the presence of the ABO alleles in these extinct humans. Results We have analysed two male Neandertals that were retrieved under controlled conditions at the El Sidron site in Asturias (Spain and that appeared to be almost free of modern human DNA contamination. We find a human specific diagnostic deletion for blood group O (O01 haplotype in both Neandertal individuals. Conclusion These results suggest that the genetic change responsible for the O blood group in humans predates the human and Neandertal divergence. A potential selective event associated with the emergence of the O allele may have therefore occurred after humans separated from their common ancestor with chimpanzees and before the human-Neandertal population divergence.

  4. Blood groups and human groups: collecting and calibrating genetic data after World War Two.

    Science.gov (United States)

    Bangham, Jenny

    2014-09-01

    Arthur Mourant's The Distribution of the Human Blood Groups (1954) was an "indispensable" reference book on the "anthropology of blood groups" containing a vast collection of human genetic data. It was based on the results of blood-grouping tests carried out on half-a-million people and drew together studies on diverse populations around the world: from rural communities, to religious exiles, to volunteer transfusion donors. This paper pieces together sequential stages in the production of a small fraction of the blood-group data in Mourant's book, to examine how he and his colleagues made genetic data from people. Using sources from several collecting projects, I follow how blood was encountered, how it was inscribed, and how it was turned into a laboratory resource. I trace Mourant's analytical and representational strategies to make blood groups both credibly 'genetic' and understood as relevant to human ancestry, race and history. In this story, 'populations' were not simply given, but were produced through public health, colonial and post-colonial institutions, and by the labour and expertise of subjects, assistants and mediators. Genetic data were not self-evidently 'biological', but were shaped by existing historical and geographical identities, by political relationships, and by notions of kinship and belonging.

  5. Transfusion reaction in a case with the rare Bombay blood group

    OpenAIRE

    Hayedeh Javadzadeh Shahshahani; Mohamad Reza Vahidfar; Seyed Ali Khodaie

    2013-01-01

    Bombay phenotype is extremely rare in Caucasian with an incidence of 1 in 250,000. When individuals with the Bombay phenotype need blood transfusion, they can receive only autologous blood or blood from another Bombay blood group. Transfusing blood group O red cells to them can cause a fatal hemolytic transfusion reaction. In this study, we report a case with the rare Bombay blood group that was misdiagnosed as the O blood group and developed a hemolytic transfusion reaction. This highlights ...

  6. Association of Acid Fast Bacilli Positive Cases with ABO Blood Groups and Frequency of Distribution of ABO Blood Groups among North Bengalis In India

    OpenAIRE

    Dilip Kumar Mishra

    2011-01-01

    Blood group in 44 cases of A.F.B positive patients and 3476 non-tubercular normal persons were determined. A relatively increased incidence of A.F.B positive cases were observed in persons with O blood group and blood group B was observed as commonest group among north Bengalis.

  7. The prognostic value of ABO blood group in cancer patients

    Science.gov (United States)

    Franchini, Massimo; Liumbruno, Giancarlo M.; Lippi, Giuseppe

    2016-01-01

    The antigens of the ABO system are expressed on red blood cell membranes as well as on the surface of several other normal and pathological cells and tissues. Following the first clinical observations more than 60 years ago, the role of ABO blood group in cancer biology has been intensely studied by several investigators, and it is now widely recognised that ABO antigens are associated with the risk of developing several types of tumours, namely pancreatic and gastric cancers. However, whether this association also affects the clinical outcome of cancer patients is less certain. In this narrative review, based on literature data, we discuss the role of ABO blood types as prognostic biomarkers in different types of cancers. The current knowledge of the underlying pathogenic mechanisms of the association is also analysed. PMID:26674825

  8. Implications of the Kidd blood group system in renal transplantation.

    Science.gov (United States)

    Rourk, A; Squires, J E

    2012-01-01

    The association of the Kidd blood group system with hemolytic transfusion reactions and hemolytic disease of the newborn is well known. The Kidd antigens, which are localized to the HUT/UT-B urea transport protein, are found on red blood cells and the endothelial cells of the blood vessels of the medulla of the kidney. Recently it has been suggested that these antigens might play a role as minor histocompatibility antigens in renal transplantation. In the current case, the appearance of an anti-Jk(b) 10 years after renal transplantation associated with early renal allograft rejection further supports the potential importance of these antigens in renal transplantation and allograft rejection. PMID:23286555

  9. The LAN blood group system:a review.

    Science.gov (United States)

    Peyrard, Thierry

    2013-01-01

    LAN (Langereis) was officially recognized by the International Society of Blood Transfusion in 2012 as being the 33rd human blood group system. It consists of one single high-prevalence antigen,Lan (LANl). The ABCB6 protein is the carrier of the Lan blood group antigen. The ABCB6 gene (chromosome 2q36, 19 exons)encodes the ABCB6 polypeptide (ATP-binding cassette protein,subfamily B, member 6), known as a porphyrin transporter.The exceptional Lan- people do not express ABCB6 (Lan null phenotype), owing to several different molecular mechanisms affecting ABCB6: frameshift leading to a premature stop codon(deletion, insertion, or nonsense mutation of nucleotides);missense mutation; or intronic mutation responsible for RNA splicing defect. Despite the Lan antigen's being reported to play a key role in erythropoiesis and detoxification of cells, Lan people do not appear to demonstrate susceptibility to any disease or seemingly physiologic disorder. Anti-Lan has been described as having variable clinical significance, either for hemolytic transfusion reactions (none to severe) or hemolytic disease of the fetus and newborn (none to mild). Despite challenging conditions caused by the scarcity of Lan- donors worldwide, Lan- blood should ideally be given to patients with anti-Lan, especially those with a high-titer antibody.

  10. Transfusion reaction in a case with the rare Bombay blood group.

    Science.gov (United States)

    Shahshahani, Hayedeh Javadzadeh; Vahidfar, Mohamad Reza; Khodaie, Seyed Ali

    2013-01-01

    Bombay phenotype is extremely rare in Caucasian with an incidence of 1 in 250,000. When individuals with the Bombay phenotype need blood transfusion, they can receive only autologous blood or blood from another Bombay blood group. Transfusing blood group O red cells to them can cause a fatal hemolytic transfusion reaction. In this study, we report a case with the rare Bombay blood group that was misdiagnosed as the O blood group and developed a hemolytic transfusion reaction. This highlights the importance of both forward and reverse typing in ABO blood grouping and standard cross-matching and performing standard pretransfusion laboratory tests in hospital blood banks. PMID:23559776

  11. Transfusion reaction in a case with the rare Bombay blood group

    Directory of Open Access Journals (Sweden)

    Hayedeh Javadzadeh Shahshahani

    2013-01-01

    Full Text Available Bombay phenotype is extremely rare in Caucasian with an incidence of 1 in 250,000. When individuals with the Bombay phenotype need blood transfusion, they can receive only autologous blood or blood from another Bombay blood group. Transfusing blood group O red cells to them can cause a fatal hemolytic transfusion reaction. In this study, we report a case with the rare Bombay blood group that was misdiagnosed as the O blood group and developed a hemolytic transfusion reaction. This highlights the importance of both forward and reverse typing in ABO blood grouping and standard cross-matching and performing standard pretransfusion laboratory tests in hospital blood banks.

  12. Transfusion reaction in a case with the rare Bombay blood group.

    Science.gov (United States)

    Shahshahani, Hayedeh Javadzadeh; Vahidfar, Mohamad Reza; Khodaie, Seyed Ali

    2013-01-01

    Bombay phenotype is extremely rare in Caucasian with an incidence of 1 in 250,000. When individuals with the Bombay phenotype need blood transfusion, they can receive only autologous blood or blood from another Bombay blood group. Transfusing blood group O red cells to them can cause a fatal hemolytic transfusion reaction. In this study, we report a case with the rare Bombay blood group that was misdiagnosed as the O blood group and developed a hemolytic transfusion reaction. This highlights the importance of both forward and reverse typing in ABO blood grouping and standard cross-matching and performing standard pretransfusion laboratory tests in hospital blood banks.

  13. 21 CFR 864.9175 - Automated blood grouping and antibody test system.

    Science.gov (United States)

    2010-04-01

    ...) Identification. An automated blood grouping and antibody test system is a device used to group erythrocytes (red blood cells) and to detect antibodies to blood group antigens. (b) Classification. Class II (performance... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Automated blood grouping and antibody test...

  14. Adult Living Donor Liver Transplantation with ABO-Incompatible Grafts: A German Single Center Experience

    Directory of Open Access Journals (Sweden)

    Armin D. Goralczyk

    2009-01-01

    Full Text Available Adult living donor liver transplantations (ALDLTs across the ABO blood group barrier have been reported in Asia, North Americas, and Europe, but not yet in Germany. Several strategies have been established to overcome the detrimental effects that are attached with such a disparity between donor and host, but no gold standard has yet emerged. Here, we present the first experiences with three ABO-incompatible adult living donor liver transplantations in Germany applying different immunosuppressive strategies. Four patient-donor couples were considered for ABO-incompatible ALDLT. In these patients, resident ABO blood group antibodies (isoagglutinins were depleted by plasmapheresis or immunoadsorption and replenishment was inhibited by splenectomy and/or B-cell-targeted immunosuppression. Despite different treatments ALDLT could safely be performed in three patients and all patients had good initial graft function without signs for antibody-mediated rejection (AMR. Two patients had long-term graft survival with stable graft function. We thus propose the feasibility of ABO-incompatible ALDLT with these protocols and advocate further expansion of ABO incompatible ALDLT in multicenter trials to improve efficacy and safety.

  15. Molecular genotyping of ABO blood groups in some population groups from India

    Directory of Open Access Journals (Sweden)

    Sabita Ray

    2014-01-01

    Full Text Available Background & objectives: Indian population is characterized by the presence of various castes and tribal groups. Various genetic polymorphisms have been used to differentiate among these groups. Amongst these, the ABO blood group system has been extensively studied. There is no information on molecular genotyping of ABO blood groups from India. Therefore, the main objective of this study was to characterize the common A, B and O alleles by molecular analysis in some Indian population groups. Methods: One hundred samples from the mixed population from Mumbai, 101 samples from the Dhodia tribe and 100 samples from the Parsi community were included in this study. Initially, the samples were phenotyped by standard serologic techniques. PCR followed by single strand conformational polymorphsim (SSCP was used for molecular ABO genotyping. Samples showing atypical SSCP patterns were further analysed by DNA sequencing to characterize rare alleles. Results: Seven common ABO alleles with 19 different genotypes were found in the mixed population. The Dhodias showed 12 different ABO genotypes and the Parsis revealed 15 different ABO genotypes with six common ABO alleles identified in each of them. Two rare alleles were also identified. Interpretation & conclusions: This study reports the distribution of molecular genotypes of ABO alleles among some population groups from India. Considering the extremely heterogeneous nature of the Indian population, in terms of various genotype markers like blood groups, red cell enzymes, etc., many more ABO alleles are likely to be encountered.

  16. Plates with Incompatible Prestrain

    OpenAIRE

    Bhattacharya, Kaushik; Lewicka, Marta; Schäffner, Mathias

    2016-01-01

    We study effective elastic behavior of the incompatibly prestrained thin plates, where the prestrain is independent of thickness and uniform through the plate’s thickness h. We model such plates as three-dimensional elastic bodies with a prescribed pointwise stress-free state characterized by a Riemannian metric G, and seek the limiting behavior as h→0. We first establish that when the energy per volume scales as the second power of h, the resulting Γ-limit is a Kirchhoff-type bending theory....

  17. Blood group genotyping: from patient to high-throughput donor screening

    NARCIS (Netherlands)

    B. Veldhuisen; C.E. van der Schoot; M. de Haas

    2009-01-01

    Blood group antigens, present on the cell membrane of red blood cells and platelets, can be defined either serologically or predicted based on the genotypes of genes encoding for blood group antigens. At present, the molecular basis of many antigens of the 30 blood group systems and 17 human platele

  18. ABO Blood Group System and Gastric Cancer: A Case-Control Study and Meta-Analysis

    OpenAIRE

    Yingyan Yu; Zhenggang Zhu; Jun Zhang; Min Yan; Bingya Liu; Jianian Zhang; Jun Ji; Zhiwei Wang; Lei Liu

    2012-01-01

    This study focuses on the association between the ABO blood group system and the risk of gastric cancer or Helicobacter pylori infection. The data for the ABO blood group was collected from 1045 cases of gastric cancer, whereby the patient underwent a gastrectomy in Ruijin Hospital, Shanghai. The information on the ABO blood group from 53,026 healthy blood donors was enrolled as control. We searched the Pubmed database on the relationship between ABO blood groups and gastric cancer risk for m...

  19. ABO Blood Group. Related Investigations and Their Association with Defined Pathologies

    Directory of Open Access Journals (Sweden)

    Ursula Jesch

    2007-01-01

    Carriers of blood group O suffered from ulcus ventriculi and gastritis (X21 = 78.629, p <0.001, colitis ulcerosa and duodenitis (X21 = 5.846, p < 0.016, whereas male patients carrying blood group A tended to contract different types of tumours. In patients with intestinal tumours, females with blood group A were more likely to develop the pathology, whereas in males, the blood group O dominated. The development of cholelithiasis was found, above all, in patients with blood group O, which differed from other research where a correlation between this pathology and blood group A was found.

  20. [Duffy blood group antigens: structure, serological properties and function].

    Science.gov (United States)

    Łukasik, Ewa; Waśniowska, Kazimiera

    2016-01-01

    Duffy (Fy) blood group antigens are located on seven-transmembrane glycoprotein expressed on erythrocytes and endothelial cells, which acts as atypical chemokine receptor (ACKR1) and malarial receptor. The biological role of the Duffy glycoprotein has not been explained yet. It is suggested that Duffy protein modulate the intensity of the inflammatory response. The Duffy blood group system consists of two major antigens, Fy(a) and Fy(b), encoded by two codominant alleles designated FY*A and FY*B which differ by a single nucleotide polymorphism (SNP) at position 125G>A of the FY gene that results in Gly42Asp amino acid change in the Fy(a) and Fy(b) antigens, respectively. The presence of antigen Fy(a) and/or Fy(b) on the erythrocytes determine three Duffy-positive phenotypes: Fy(a+b-), Fy(a-b+) and Fy(a+b+), identified in Caucasian population. The Duffy-negative phenotype Fy(a-b-), frequent in Africans, but very rare in Caucasians, is defined by the homozygous state of FY*B-33 alleles. The FY*B-33 allele is associated with a SNP -33T>C in the promoter region of the FY gene, which suppresses erythroid expression of this gene without affecting its expression in other tissues. The FY*X allele, found in Caucasians, is correlated with weak expression of Fy(b) antigen. Fy(x) antigen differs from the native Fy(b) by the Arg89Cys and Ala100Thr amino acid substitutions due to SNPs: 265C>T and 298G>A in FY*B allele. The frequency of the FY alleles shows marked geographic disparities, the FY*B-33 allele is predominant in Africans, the FY*B in Caucasians, while the FY*A allele is dominant in Asians and it is the most prevalent allele globally. PMID:26943312

  1. Duffy blood group antigens: structure, serological properties and function

    Directory of Open Access Journals (Sweden)

    Ewa Łukasik

    2016-03-01

    Full Text Available Duffy (Fy blood group antigens are located on seven-transmembrane glycoprotein expressed on erythrocytes and endothelial cells, which acts as atypical chemokine receptor (ACKR1 and malarial receptor. The biological role of the Duffy glycoprotein has not been explained yet. It is suggested that Duffy protein modulate the intensity of the inflammatory response. The Duffy blood group system consists of two major antigens, Fya and Fyb, encoded by two codominant alleles designated FY*A and FY*B which differ by a single nucleotide polymorphism (SNP at position 125G>A of the FY gene that results in Gly42Asp amino acid change in the Fya and Fyb antigens, respectively. The presence of antigen Fya and/or Fyb on the erythrocytes determine three Duffy-positive phenotypes: Fy(a+b-, Fy(a-b+ and Fy(a+b+, identified in Caucasian population. The Duffy-negative phenotype Fy(a-b-, frequent in Africans, but very rare in Caucasians, is defined by the homozygous state of FY*B-33 alleles. The FY*B-33 allele is associated with a SNP -33T>C in the promoter region of the FY gene, which suppresses erythroid expression of this gene without affecting its expression in other tissues. The FY*X allele, found in Caucasians, is correlated with weak expression of Fyb antigen. Fyx antigen differs from the native Fyb by the Arg89Cys and Ala100Thr amino acid substitutions due to SNPs: 265C>T and 298G>A in FY*B allele. The frequency of the FY alleles shows marked geographic disparities, the FY*B-33 allele is predominant in Africans, the FY*B in Caucasians, while the FY*A allele is dominant in Asians and it is the most prevalent allele globally. Tytuł główny Tak

  2. Effect of different proportions of mixed blood exchange transfusion on blood circulation in neonates with ABO incompatibility hemolytic disease%不同比例成分血对新生儿母子ABO血型不合溶血病换血治疗后内环境影响研究

    Institute of Scientific and Technical Information of China (English)

    李军; 黄瑞文; 胡月圆; 肖艾青; 廖镇宇

    2011-01-01

    Objective To explore the effect of different proportions (the O type RBCs vs the AB type plasma)of mixed blood exchange transfusion on blood circulation in neonates with ABO incompatibility hemolytic disease. Methods From Jan. 2009 to Apr. 2010 in Hunan Children's Hospital,forty newborn infants with the disease, treated by exchange transfusion with different proportions of mixed blood (AB type plasma was mixed with 0 type red blood cell), were randomly divided into two groups: 16 patients in treatment group, who used 0 type red blood cells and AB type plasma with the proportion of 3 : 1 ( the 0 type RBCs 300 mL: the AB type plasma 100 mL) during blood transfusion; 24 patients in control group with the proportion of 2: 1. Both of the two groups received the double exchange transfusion in accordance with 150 ~ 180 mL/kg. After exchange, all the patients in controll group received 0 type RBCs transfusion with 15 ~ 20 mlAg (total volume was 60mL), while the other group did not. The other therapies in both of the two groups were the same.The indicators, which included the exchange rate of neonatal serum bilirubin, RBC, hemoglobin (Hb), hematocrit (Hct) , the elctrocyte and gas analysis, were monitored before and after the exchange transfusion. Results The total bilirubin of the two groups were lower after the exchange transfusion, and that the treatment group was even lower(P 0.05), while in the control group the difference was significant (P 0.05). Conclusion The exchange transfusion, using 0 type red blood cells and AB type plasma with the proportion of 3 : 1 inhemolytic disease, can not only decrease the level of bilirubin in blood rapidly but also avoid the anemia.The serum electrolytes and acid-base balance have no signifi-cant differences. It is worth promoting.%目的 探讨不同比例成分血对新生儿母子ABO血型不合溶血病(HDN)换血治疗后内环境的影响.方法 对2009年1月至2010年4月湖南省儿童医院40例HDN达到换血指征的

  3. Assessing ABO/Rh Blood Group Frequency and Association with Asymptomatic Malaria among Blood Donors Attending Arba Minch Blood Bank, South Ethiopia.

    Science.gov (United States)

    Alemu, Getaneh; Mama, Mohammedaman

    2016-01-01

    Background. Determination of the various ABO/Rh blood group distributions and their association with malaria infection has paramount importance in the context of transfusion medicine and malaria control. Methods. Facility based cross-sectional study was conducted from February to June, 2015, to assess ABO/Rh blood groups distribution and their association with asymptomatic malaria. A structured questionnaire was used to collect data. Blood grouping was done using monoclonal antibodies. Thin and thick blood films were examined for Plasmodium parasites. Data were analyzed using SPSS version 20.0. Results. A total of 416 blood donors participated with median age of 22 ± 0.29 (median ± standard error of the mean). Distribution of ABO phenotypes, in decreasing order, was O (175, 42.1%), A (136, 32.7%), B (87, 20.9%), and AB (18, 4.3%). Most of them were Rh+ (386, 92.8%). The overall malaria prevalence was 4.1% (17/416). ABO blood group is significantly associated with malaria infection (P = 0.022). High rate of parasitemia was seen in blood group O donors (6.899, P = 0.003) compared to those with other ABO blood groups. Conclusion. Blood groups O and AB phenotypes are the most and the least ABO blood groups, respectively. There is significant association between ABO blood group and asymptomatic malaria parasitemia.

  4. Assessing ABO/Rh Blood Group Frequency and Association with Asymptomatic Malaria among Blood Donors Attending Arba Minch Blood Bank, South Ethiopia.

    Science.gov (United States)

    Alemu, Getaneh; Mama, Mohammedaman

    2016-01-01

    Background. Determination of the various ABO/Rh blood group distributions and their association with malaria infection has paramount importance in the context of transfusion medicine and malaria control. Methods. Facility based cross-sectional study was conducted from February to June, 2015, to assess ABO/Rh blood groups distribution and their association with asymptomatic malaria. A structured questionnaire was used to collect data. Blood grouping was done using monoclonal antibodies. Thin and thick blood films were examined for Plasmodium parasites. Data were analyzed using SPSS version 20.0. Results. A total of 416 blood donors participated with median age of 22 ± 0.29 (median ± standard error of the mean). Distribution of ABO phenotypes, in decreasing order, was O (175, 42.1%), A (136, 32.7%), B (87, 20.9%), and AB (18, 4.3%). Most of them were Rh+ (386, 92.8%). The overall malaria prevalence was 4.1% (17/416). ABO blood group is significantly associated with malaria infection (P = 0.022). High rate of parasitemia was seen in blood group O donors (6.899, P = 0.003) compared to those with other ABO blood groups. Conclusion. Blood groups O and AB phenotypes are the most and the least ABO blood groups, respectively. There is significant association between ABO blood group and asymptomatic malaria parasitemia. PMID:26925291

  5. Evolutionary genetics of the human Rh blood group system.

    Science.gov (United States)

    Perry, George H; Xue, Yali; Smith, Richard S; Meyer, Wynn K; Calışkan, Minal; Yanez-Cuna, Omar; Lee, Arthur S; Gutiérrez-Arcelus, María; Ober, Carole; Hollox, Edward J; Tyler-Smith, Chris; Lee, Charles

    2012-07-01

    The evolutionary history of variation in the human Rh blood group system, determined by variants in the RHD and RHCE genes, has long been an unresolved puzzle in human genetics. Prior to medical treatments and interventions developed in the last century, the D-positive (RhD positive) children of D-negative (RhD negative) women were at risk for hemolytic disease of the newborn, if the mother produced anti-D antibodies following sensitization to the blood of a previous D-positive child. Given the deleterious fitness consequences of this disease, the appreciable frequencies in European populations of the responsible RHD gene deletion variant (for example, 0.43 in our study) seem surprising. In this study, we used new molecular and genomic data generated from four HapMap population samples to test the idea that positive selection for an as-of-yet unknown fitness benefit of the RHD deletion may have offset the otherwise negative fitness effects of hemolytic disease of the newborn. We found no evidence that positive natural selection affected the frequency of the RHD deletion. Thus, the initial rise to intermediate frequency of the RHD deletion in European populations may simply be explained by genetic drift/founder effect, or by an older or more complex sweep that we are insufficiently powered to detect. However, our simulations recapitulate previous findings that selection on the RHD deletion is frequency dependent and weak or absent near 0.5. Therefore, once such a frequency was achieved, it could have been maintained by a relatively small amount of genetic drift. We unexpectedly observed evidence for positive selection on the C allele of RHCE in non-African populations (on chromosomes with intact copies of the RHD gene) in the form of an unusually high F( ST ) value and the high frequency of a single haplotype carrying the C allele. RhCE function is not well understood, but the C/c antigenic variant is clinically relevant and can result in hemolytic disease of the

  6. Prevalence of feline blood groups in the Montreal area of Quebec, Canada

    OpenAIRE

    Fosset, Fabrice T.J.; Blais, Marie-Claude

    2014-01-01

    The feline AB blood group system has clinical significance because type B cats have natural alloimmune anti-A antibodies which can cause isoerythrolysis of the newborn and life-threatening transfusion reactions. In the United States, the prevalence of type B blood is estimated to be 1% to 2%. This study determined the prevalence of feline AB blood groups among 207 potential blood donor cats that included 178 domestic cats, in the Montreal area of Quebec, Canada. Blood typing was performed usi...

  7. Clinico-serologic co-relation in bi-directional ABO incompatible hemopoietic stem cell transplantation

    Directory of Open Access Journals (Sweden)

    Sabita Basu

    2015-01-01

    Full Text Available Background: The ABO blood group system is of prime significance in red cell transfusion and organ transplantation. However, ABO compatibility is not critical in allogenic hemopoietic stem cell transplantation (HSCT and approximately 40-50% of hemopoietic stem cell transplants are ABO incompatible. This incompatibility may be major, minor or bi-directional. Though there are descriptions of transfusion practice and protocols in ABO incompatible HSCT, there are considerable variations and transfusion support in these patients can be very challenging. Aims: The immunohematologic observations in two cases of bi-directional ABO incompatible HSCT have been described, and clinico-serologic correlation has been attempted. Materials and Methods: In both cases, peripheral blood stem cell harvests were obtained using the Cobe spectra cell separator. Immunohematologic assessments in the donor and recipient were done as a part of pre HSCT evaluation. Both the standard tube technique and column agglutination method (Ortho Biovue Micro Bead System was used. Antibody screen was done by column agglutination method using three cell panel (Surgiscreen cells. Isoagglutinin titration was done by the master dilution method and standard validated techniques were used. Results: The pattern of laboratory findings in the two cases was different and so were the clinical outcomes. Although there was early engraftment in the first case, the second case developed pure red cell aplasia and this was well-reflected in the immunohematologic assessments. Conclusion: Immunohematologic assessment correlated well with the clinical picture and could be used to predict clinical outcome and onset of complications in ABO incompatible HSCT.

  8. Modifying the red cell surface: towards an ABO-universal blood supply

    DEFF Research Database (Denmark)

    Olsson, Martin L; Clausen, Henrik

    2007-01-01

    Eliminating the risk for ABO-incompatible transfusion errors and simplifying logistics by creating a universal blood inventory is a challenging idea. Goldstein and co-workers pioneered the field of enzymatic conversion of blood group A and B red blood cells (RBCs) to O (ECO). Using alpha-galactos......Eliminating the risk for ABO-incompatible transfusion errors and simplifying logistics by creating a universal blood inventory is a challenging idea. Goldstein and co-workers pioneered the field of enzymatic conversion of blood group A and B red blood cells (RBCs) to O (ECO). Using alpha...

  9. Frequency and Distribution of Blood Groups in Blood Donors in Western Ahmedabad AND#8211; A Hospital Based Study

    Directory of Open Access Journals (Sweden)

    Patel Piyush A

    2012-04-01

    Full Text Available Background: Up till now about 400 red cells antigen have been identified. The majority are inherited by Mendelian fashion. The ABO and Rhesus (Rh blood group system are most important for blood transfusion purposes, parental testing, legal medicine and in population genetic study. Objective: This study was conducted to determine and compare the frequency of ABO and Rh blood groups in blood donors in secondary care teaching hospital at Western Ahmedabad, Gujarat, India. Materials and Methods: A retrospective study was conducted at Blood bank, GMERS Medical College, Sola, Ahmedabad over a period of seven years from 1st January 2005 to 31st December 2011. Blood group of the blood donors was determined by commercially available standard monoclonal antisera by test tube agglutination technique. Results & conclusion: Out of 5316 subjects, 5076 (95.48% were male and 240 (4.52% were female subjects. The commonest ABO blood group present was B (39.40 % followed by O (30.79 %, A (21.94 % and AB (7.86 % in blood donors; while in Rhesus system, 5053(95.05% donors were Rh-positive and 263(4.95% donors were Rh-negative. The study has a significant implication regarding the inventory management of blood bank and transfusion services for the patient admitted in our secondary care teaching hospital. [National J of Med Res 2012; 2(2.000: 202-206

  10. Structure of ganglioside with CAD blood group antigen activity

    Energy Technology Data Exchange (ETDEWEB)

    Gillard, B.K.; Blanchard, D.; Cartron, J.P.; van Kuik, G.A.; Vliegenthart, J.F.G.; Marcus, D.M.

    1986-05-01

    The novel erythrocyte ganglioside which carries the blood group Cad determinant has been isolated, and its structure has been determined. The ganglioside contained Glu:Gal:GalNAc:GlcNAc in a molar ratio of 1.00:1.94:0.93:0.95. The ganglioside binds Helix pomatia lectin and its chromatographic mobility is similar to G/sub D3/. After treatment with ..beta..-hexosaminidase (human placenta HexA) the product migrated with sialosylparagloboside (SPG), no longer binds Helix lectin, and binds a human anti-SPG antibody. Treatment of this material with neuraminidase (V. cholera) yielded a product with the mobility of paragloboside that bound monoclonal antibody 1B2. NMR analysis revealed that the terminal GalNAc is linked ..beta..1-4 to Gal, and confirms the structure proposed previously: GalNAc..beta..1-4(NeuAc..cap alpha..2-3)Gal..beta..1-4GlcNAc..beta..1-3Gal..beta..1-4Glc-Cer. This structure is consistent with the previous demonstration that a compound with the same chromatographic mobility as the Cad ganglioside could be synthesized by enzymatic transfer of GalNAc to sialosylparagloboside.

  11. Blood group variation in the Isle of Lewis.

    Science.gov (United States)

    Clegg, E J; Tills, D; Warlow, A; Wilkinson, J; Marin, A

    1985-01-01

    Blood groups and protein and enzyme polymorphism distributions were studied in 285 residents on the Isle of Lewis, in the Outer Hebrides. As well as gene frequency calculations for individual loci, genetic distance estimations were made and a phylogenetic tree was constructed. The results indicated several major differences from North-west European populations, with high values of R2(CDe), Rz(CDE) and P1. Among protein and enzyme polymorphisms Hp1, EAPA and PGM1(1) had very high frequencies. Genetic distances show Lewis to be unlike both Western and Eastern North European populations, while the phylogenetic tree shows a common, but rather distant, ancestry with Icelanders. This genetic uniqueness of Lewis as a whole is accompanied by a considerable degree of heterogeneity within the island itself, especially in the ABO and Rh systems. Stornoway, with a greater proportion of residents descended from immigrant stock, shows a greater degree of similarity with neighbouring populations. The reasons for both the overall uniqueness and the heterogeneity within Lewis are discussed, but in the absence of a large time-depth and adequate vital records, the various roles of selection, drift and migration in producing them are difficult to establish. PMID:3929670

  12. ABO blood group and breast cancer incidence and survival

    OpenAIRE

    Gates, Margaret A.; Xu, Mousheng; Chen, Wendy Y.; Kraft, Peter; Hankinson, Susan E; Wolpin, Brian M.

    2012-01-01

    ABO blood type has been associated with risk and survival for several malignancies; however, data for an association with breast cancer are inconsistent. Our study population consisted of Nurses’ Health Study participants with self-reported serologic blood type and/or ABO genotype. Using Cox proportional hazards regression, we examined the association between serologic blood type and incident breast cancer among 67,697 women, including 3,107 cases. In addition, we examined the association wit...

  13. PP13, maternal ABO blood groups and the risk assessment of pregnancy complications.

    Directory of Open Access Journals (Sweden)

    Nandor Gabor Than

    Full Text Available BACKGROUND: Placental Protein 13 (PP13, an early biomarker of preeclampsia, is a placenta-specific galectin that binds beta-galactosides, building-blocks of ABO blood-group antigens, possibly affecting its bioavailability in blood. METHODS AND FINDINGS: We studied PP13-binding to erythrocytes, maternal blood-group effect on serum PP13 and its performance as a predictor of preeclampsia and intrauterine growth restriction (IUGR. Datasets of maternal serum PP13 in Caucasian (n = 1078 and Hispanic (n = 242 women were analyzed according to blood groups. In vivo, in vitro and in silico PP13-binding to ABO blood-group antigens and erythrocytes were studied by PP13-immunostainings of placental tissue-microarrays, flow-cytometry of erythrocyte-bound PP13, and model-building of PP13--blood-group H antigen complex, respectively. Women with blood group AB had the lowest serum PP13 in the first trimester, while those with blood group B had the highest PP13 throughout pregnancy. In accordance, PP13-binding was the strongest to blood-group AB erythrocytes and weakest to blood-group B erythrocytes. PP13-staining of maternal and fetal erythrocytes was revealed, and a plausible molecular model of PP13 complexed with blood-group H antigen was built. Adjustment of PP13 MoMs to maternal ABO blood group improved the prediction accuracy of first trimester maternal serum PP13 MoMs for preeclampsia and IUGR. CONCLUSIONS: ABO blood group can alter PP13-bioavailability in blood, and it may also be a key determinant for other lectins' bioavailability in the circulation. The adjustment of PP13 MoMs to ABO blood group improves the predictive accuracy of this test.

  14. Frequency of ABO/Rhesus Blood Groups in Patients with Diabetes Mellitus.

    Science.gov (United States)

    Oner, Can; Dogan, Burcu; Telatar, Berrin; Celik Yagan, Canan Fidan; Oguz, Aytekin

    2016-01-01

    The correlation between ABO/Rh blood groups and diabetes mellitus is still controversial. The aim of this study was to determine the relationship between ABO/Rhesus blood groups and diabetes in Turkish population. This cross-sectional study was conducted in Istanbul Medeniyet University Göztepe Education and Training Hospital's Diabetes Units. The study group was composed of 421 patients with type-1 diabetes, 484 patients with type-2 diabetes and 432 controls. Blood samples were collected and tested for ABO/Rhesus blood groups. Data was analyzed by SPSS version 17.0. A significant association was found between blood groups and diabetes mellitus. The frequency of AB blood group was significantly higher in type-1 diabetics; and A blood group was significantly higher in type-2 diabetics. Furthermore, Rh negativity were significantly more frequent in type-2 diabetics.

  15. Plates with Incompatible Prestrain

    Science.gov (United States)

    Bhattacharya, Kaushik; Lewicka, Marta; Schäffner, Mathias

    2016-07-01

    We study effective elastic behavior of the incompatibly prestrained thin plates, where the prestrain is independent of thickness and uniform through the plate's thickness h. We model such plates as three-dimensional elastic bodies with a prescribed pointwise stress-free state characterized by a Riemannian metric G, and seek the limiting behavior as {h to 0}. We first establish that when the energy per volume scales as the second power of h, the resulting {Γ} -limit is a Kirchhoff-type bending theory. We then show the somewhat surprising result that there exist non-immersible metrics G for whom the infimum energy (per volume) scales smaller than h 2. This implies that the minimizing sequence of deformations carries nontrivial residual three-dimensional energy but it has zero bending energy as seen from the limit Kirchhoff theory perspective. Another implication is that other asymptotic scenarios are valid in appropriate smaller scaling regimes of energy. We characterize the metrics G with the above property, showing that the zero bending energy in the Kirchhoff limit occurs if and only if the Riemann curvatures R 1213, R 1223 and R 1212 of G vanish identically. We illustrate our findings with examples; of particular interest is an example where {G_{2 × 2}}, the two-dimensional restriction of G, is flat but the plate still exhibits the energy scaling of the Föppl-von Kármán type. Finally, we apply these results to a model of nematic glass, including a characterization of the condition when the metric is immersible, for {G = Id3 + γ n ⊗ n} given in terms of the inhomogeneous unit director field distribution { n in R^3}.

  16. Distribution of ABO and rhesus (D blood group antigens among blood donors at a tertiary care teaching hospital blood bank in south India

    Directory of Open Access Journals (Sweden)

    Suresh B

    2015-04-01

    Full Text Available Background: The ABO and Rhesus (Rh blood group systems are important for transfusion of blood and its components, organ transplantation, genetic studies and in medico-legal issues. Despite the long list of several other blood groups discovered so far, the knowledge and distribution of ABO and Rh-D blood group are essential for effective management of blood bank inventory. Methods: We retrospectively studied the distribution of ABO and Rh blood group antigens in donors presenting to our tertiary care teaching hospital blood bank in south India during the period January 2007 to August 2014. Blood group was determined by commercially available standard monoclonal antisera by test tube agglutination technique. Results: A total of 49,110 donor samples were tested during the study period for ABO grouping and Rh-D typing. Out of these 96.9% were males. The frequency of O, B, A, AB and Bombay blood groups were 41.7%, 32.2% 20%, 6.1% and 0.03% respectively. Rh (D positive and negative blood groups were seen in 92.8% and 7.2% respectively. The allele frequencies of the I A , IB and IO alleles were 0.1398, 0.2148 and 0.6454 respectively. In case of Rh-D group, the calculated gene frequencies for ID and Id were 0.7321 and 0.2679 respectively. Conclusion: Knowledge of blood group systems as documented in the present study helps in efficient management of blood bank and transfusion services in emergencies.

  17. ABO blood groups and oral premalignancies: A clinical study in selected Indian population.

    Science.gov (United States)

    Bhateja, S; Arora, G

    2014-01-01

    Background: The ABO blood group antigens are present on the surface of red blood cells and various epithelial cells. As the majority of human cancers are derived from epithelial cells, changes in blood group antigens constitute an important aspect of human cancers. The aim of the study was to establish clinical usefulness of ABO blood group as a predisposing factor in early diagnosis and management of patients with oral precancerous lesions/conditions. Materials and Methods: The study sample consisted of 50 control and 50 oral precancer (25 leukoplakia and 25 Oral Submucous Fibrosis) confirmed by histopathologic examination. All samples were subjected to blood group testing and their prevalence was compared by Z-test using STATA version 8. Results: The "A" blood group was prevalent among the precancerous group. Significant differences on prevalences of blood groups were found (P blood group. Conclusion: Blood group type should be considered along with other risk factors to understand the individual patient's risk and further studies in larger samples with inclusion of Rh factor is needed to elucidate the relationship with ABO blood group types.

  18. Binding of the blood group-reactive lectins to human adult kidney specimens.

    Science.gov (United States)

    Laitinen, L; Juusela, H; Virtanen, I

    1990-01-01

    The binding of a panel of blood group-reactive lectins to frozen sections of human kidney was studied with a special emphasis on reactivity with endothelia and basement membranes. The blood group A-reactive lectins, all specific for alpha-D-N-acetylgalactosamine (GalNAc), Helix aspersa (HAA), Helix pomatia (HPA), and Griffonia simplicifolia I-A4 (GSA-I-A4) agglutinins bound to the endothelium in specimens with blood groups A and AB. In other samples, these lectins reacted predominantly with tubular basement membranes, as well as with certain tubules. Both Dolichos biflorus (DBA) and Vicia villosa agglutinins (VVA), reported to react with blood group A1 substance, failed to reveal endothelia in most specimens, but bound differently to tubules in all blood groups. The blood group B-reactive lectins, specific for alpha-D-galactose (alpha-Gal) or GalNAc, respectively, GSA-I-B4 and Sophora japonica agglutinin (SJA), bound to the endothelia in specimens from blood group B or AB and in other specimens bound only to certain tubules. Among the blood group O-reactive lectins, specific for alpha-L-fucose (Fuc), Ulex europaeus I agglutinin (UEA-I) conjugates, but not other lectins with a similar nominal specificity, bound strongly to endothelia in specimens with blood group O. The UEA-I conjugates bound distinctly more faintly to endothelia in specimens of other blood groups. The present results indicate that lectins, binding to defined blood group determinants, react with endothelia in specimens of the respective blood group status. Furthermore, they suggest that basement membranes and some tubules in the human kidney show a distinct heterogeneity in their expression of saccharide residues, related to their blood group status.

  19. Genetic polymorphism of blood groups and erythrocytes enzymes in population groups of the Republic of Macedonia.

    Science.gov (United States)

    Efremovska, Lj; Schmidt, H D; Scheil, H G; Gjorgjevic, D; Nikoloska Dadic, E

    2007-12-01

    This study presents the results of an examination of 3 blood-group systems (ABO, Rhesus, and P1) and erythrocyte enzymes (ADA, AK, ALADH, PGD, SAHH, PGM1, PGM3, GPT, GOT, ACP, UMPK, ESD and GLO) in populations that reside in R. Macedonia. Four population samples from the Republic of Macedonia (129 Macedonians from Skopje, 98 Albanians from Skopje, 95 Aromanians from Krusevo, 102 Aromanians from Stip) were included in the study. A comparison of the obtained results with data from literature on other Balkan populations has been made. The results of the comparison of the studied alleles indicate relatively small genetic distances among the studied populations. The obtained dendrograms indicate a larger homogeneity in the large Balkan populations, and a manifest trend of separating the Aromanian population of the Stip region. A larger separation is characteristic in the Greek population of Thrace.

  20. Frequency of abo blood groups among the diabetes mellitus type 2 patients

    International Nuclear Information System (INIS)

    Objective: To study the frequency of ABO blood groups among diabetes mellitus type 2. Results: Comparison of blood groups frequency between the general population and diabetes type 2 patients was carried out in term of percentage. It was noticed that the values were 4.36, 17.15 and 7.34% higher for A, B and AB blood groups respectively in the diabetic patients. On the contrary, the value was 28.94% lower for the blood group O. Conclusion: Present study has supported the hypothesis that diabetes mellitus type 2 and blood groups are interrelated because of the broad genetic immunologic basis in both. It is concluded that the frequency of blood groups B and O is significantly higher and lower respectively in the diabetes mellitus type 2 patients as compared to the general population. (author)

  1. Mortality and cancer in relation to ABO blood group phenotypes in the Golestan Cohort Study

    OpenAIRE

    Etemadi, Arash; Kamangar, Farin; Islami, Farhad; Poustchi, Hossein; Pourshams, Akram; Brennan, Paul; Boffetta, Paolo; Malekzadeh, Reza; Dawsey, Sanford M.; Abnet, Christian C.; Emadi, Ashkan

    2015-01-01

    Background A few studies have shown an association between blood group alleles and vascular disease, including atherosclerosis, which is thought to be due to the higher level of von Willebrand factor in these individuals and the association of blood group locus variants with plasma lipid levels. No large population-based study has explored this association with overall and cause-specific mortality. Methods We aimed to study the association between ABO blood groups and overall and cause-specif...

  2. No Association of Phenotypic ABO Blood Group and Malaria during Pregnancy

    OpenAIRE

    Boel, Machteld E.; Marcus J Rijken; Pimanpanarak, Mupawjay; Keereecharoen, Naw Lily; Proux, Stephane; Nosten, François; McGready, Rose

    2012-01-01

    In a few small studies an association between blood group O and placental malaria has been described. The relationship between blood group and malaria in pregnancy (Plasmodium vivax and Plasmodium falciparum) was analyzed in 1,468 women from three longitudinal cohort studies in which weekly malaria screening was done systematically during pregnancy. One-third of women (447 of 1,468) had at least one malaria infection in pregnancy. The ABO blood group phenotype was not associated with the spec...

  3. Relationship between ABO blood groups and malaria with clinical outcome in rural area of South India

    OpenAIRE

    Gayathri B.N.; Harendra Kumar M.L.; Gomathi. N.; Jeevan Shetty; Reethesh R. P.

    2013-01-01

    Background A number of studies have shown that susceptibility to several infectious diseases is related to the patient’s blood group. Although the relationship between blood group and susceptibility to malaria has been studied by several researchers, the results have been contradictory. Since malaria has re-emerged as a major problem in India during the past few years, it would be useful to know whether there is any relationship between blood group and infection. Objectives The study will be...

  4. Noninvasive Antenatal Determination of Fetal Blood Group Using Next-Generation Sequencing

    DEFF Research Database (Denmark)

    Rieneck, Klaus; Clausen, Frederik Banch; Dziegiel, Morten Hanefeld

    2015-01-01

    Hemolytic disease of the fetus and newborn (HDFN) is a condition characterized by a decreased lifespan of fetal red blood cells caused by maternally produced allospecific antibodies transferred to the fetus during pregnancy. The antibodies bind to the corresponding blood group antigens on fetal red...... blood cells and induce hemolysis. Cell-free DNA derived from the conceptus circulates in maternal blood. Using next-generation sequencing (NGS), it can be determined if this cell-free fetal DNA encodes the corresponding blood group antigen that is the target of the maternal allospecific antibodies....... This determination carries no risk to the fetus. It is important to determine if the fetus is at risk of hemolysis to enable timely intervention. Many tests for blood groups are based solely on the presence or absence of a single nucleotide polymorphism (SNP). Antenatal determination of fetal blood group by NGS...

  5. ABO and Rh-D blood group frequency and distribution: a tertiary care hospital experience

    Directory of Open Access Journals (Sweden)

    Pandu Rangarao Sanagapati

    2015-08-01

    Results: Out of 1740 blood donors, 1702 (55.6% were male and 38 (44.4% were female. Majority of blood donors were in 21-40 years of age group. The most frequent blood group positions in the descending order are and lsquo;O', and lsquo;A', and lsquo;B' and and lsquo;AB' respectively. One group was and lsquo;Oh' (Bombay Phenotype. Conclusions: and lsquo;O' group is the most frequent position of ABO blood group system followed by and lsquo;A' group. Rh+ is the most frequent group than Rh- in the Rh system. Blood donations by females are very low. [Int J Res Med Sci 2015; 3(8.000: 2058-2061

  6. The prevalence of transfusion transmitted infections in ABO blood groups and Rh type system

    OpenAIRE

    Jitendra Singh Nigam; Savitri Singh; Viplesh Kaur; Sumit Giri; Ravi Prakash Kaushal

    2014-01-01

    Screening of blood and blood products is important to reduce the risk of transfusion transmitted infections (TTIs). The transfusion of unscreened or inadequately screened blood and blood products are the major source of TTIs. The aim of this paper is to find out the prevalence of TTIs in ABO blood groups and Rh type system. A total of 4128 blood donors were screened from January 2010 to April 2014. Serological tests were performed for hepatitis B surface antigen (HBsAg), anti hepatitis C viru...

  7. Quantum Incompatibility in Collective Measurements

    Directory of Open Access Journals (Sweden)

    Claudio Carmeli

    2016-09-01

    Full Text Available We study the compatibility (or joint measurability of quantum observables in a setting where the experimenter has access to multiple copies of a given quantum system, rather than performing the experiments on each individual copy separately. We introduce the index of incompatibility as a quantifier of incompatibility in this multi-copy setting, as well as the notion of the compatibility stack representing various compatibility relations present in a given set of observables. We then prove a general structure theorem for multi-copy joint observables and use it to prove that all abstract compatibility stacks with three vertices have realizations in terms of quantum observables.

  8. Is ABO blood group truly a risk factor for thrombosis and adverse outcomes?

    OpenAIRE

    Zhou, Shan; Welsby, Ian

    2014-01-01

    ABO blood type is one of the most readily available laboratory tests, and serves as a vital determinant in blood transfusion and organ transplantation. The ABO antigens are expressed not only on red blood cell membranes, determining the compatibility of transfusion, but also on the surface of other human cells, including epithelium, platelet and vascular endothelium, therefore extending the research into other involvements of cardiovascular disease and postoperative outcomes. ABO blood group ...

  9. ABO blood grouping from hard and soft tissues of teeth by modified absorption-elution technique

    Directory of Open Access Journals (Sweden)

    B K Ramnarayan

    2013-01-01

    Full Text Available Background: Teeth have always been known as stable tissue that can be preserved both physically and chemically for long periods of time. Blood group substances have been known to be present in both the hard and soft tissues of the teeth. Objectives: This study aimed at detection of ABO blood group substances from soft and hard tissues of teeth and also to evaluate the reliability of teeth stored for a relatively long period as a source of blood group substances by absorption-elution technique with some modifications. Results: Blood group obtained from the teeth was compared with those obtained from the blood sample. Pulp showed a very large correlation in both fresh and long-standing teeth though it decreased slightly in the latter. Hard tissue showed a large correlation in both the groups indicating that hard tissue is quite reliable to detect blood group and that there is no much difference in the reliability in both the groups. However, combining pulp and hard tissue, correlation is moderate. Correlation of blood grouping with the age, sex, and jaw distribution was carried out. Conclusion: Blood group identification from hard and soft tissues of teeth aids in the identification of an individual.

  10. Pattern of distribution of blood group antigens on human epidermal cells during maturation

    DEFF Research Database (Denmark)

    Dabelsteen, Erik; Buschard, Karsten; Hakomori, Sen-Itiroh

    1984-01-01

    The distribution in human epidermis of A, B, and H blood group antigens and of a precursor carbohydrate chain, N-acetyl-lactosamine, was examined using immunofluorescence staining techniques. The material included tissue from 10 blood group A, 4 blood group B, and 9 blood group O persons. Murine...... on the lower spinous cells whereas H antigen was seen predominantly on upper spinous cells or on the granular cells. Epithelia from blood group A or B persons demonstrated A or B antigens, respectively, but only if the tissue sections were trypsinized before staining. In such cases A or B antigens were found...... monoclonal antibodies were used to identify H antigen (type 2 chain) and N-acetyl-lactosamine. Human antisera were used to identify A and B antigens. In all groups N-acetyl-lactosamine and H antigen were found on the cell membranes of the spinous cell layer. N-acetyl-lactosamine was present mainly...

  11. Validation of a blood group genotyping method based on high-resolution melting curve analysis.

    Science.gov (United States)

    Gong, Tianxiang; Hong, Ying; Wang, Naihong; Fu, Xuemei; Zhou, Changhua

    2014-01-01

    The detection of polymorphism is the basis of blood group genotyping and phenotype prediction. Genotyping may be useful to determine blood groups when serologic results are unclear. The development and application of different methods for blood group genotyping may be needed as a substitute for blood group typing. The purpose of this study is to establish an approach for blood group genotyping based on a melting curve analysis of real-time polymerase chain reaction (PCR). Using DNA extracted from whole blood, we developed and validated a DNA typing method for detecting DO*01/DO*02, DO*01/DI*02, LU*01/LU*02, and GYPB*03/GYBP*04 alleles using a melting curve analysis. All assays were confirmed with a commercial reagent containing sequence-specific primers (PCR-SSP), and a cohort of the samples was confirmed with sequencing. Results for all blood groups were within the range of specificity and assay variability. Genotypes of 300 blood donors were fully consistent with PCR-SSP data. The obtained genotype distribution is in complete concordance with existing data for the Chinese population. There are several advantages for this approach of blood group genotyping: lower contamination rates with PCR products in this laboratory, ease of performance, automation potential, and rapid cycling time.

  12. Exploring the Impact of Students' Learning Approach on Collaborative Group Modeling of Blood Circulation

    Science.gov (United States)

    Lee, Shinyoung; Kang, Eunhee; Kim, Heui-Baik

    2015-01-01

    This study aimed to explore the effect on group dynamics of statements associated with deep learning approaches (DLA) and their contribution to cognitive collaboration and model development during group modeling of blood circulation. A group was selected for an in-depth analysis of collaborative group modeling. This group constructed a model in a…

  13. Un caso de grupo sanguíneo raro: fenotipo p A rare blood group: p phenotype

    Directory of Open Access Journals (Sweden)

    Carlos D. De La Vega Elena

    2009-12-01

    Full Text Available Un individuo con un fenotipo eritrocitario raro carece de uno o varios antígenos presentes en la mayor parte de la población de pertenencia. Cuando presenta el anticuerpo correspondiente, se pueden producir complicaciones perinatales, transfusionales y/o transplantológicas. Se presenta el caso de una embarazada aloinmunizada derivada a nuestro servicio en la semana 12 de su tercera gesta para su evaluación y seguimiento. El diagnóstico inmunohematológico le asignó el excepcional fenotipo "p" (aproximadamente 1/200 000 individuos, asociado con una mayor tasa de abortos espontáneos y a reacciones transfusionales graves cuando se transfunden unidades incompatibles. El estudio del gen A4GALT demostró la presencia de la mutación c.752C > T en doble dosis. Esta mutación lleva a un cambio de una prolina por una leucina en el residuo 251 de la 4-α-galactosiltransferasa. Por parto inducido por sufrimiento fetal, nace a las 36 semanas una bebé con prueba de antiglobulina (Coombs directa negativa, eluido reactivo, con ictericia que requirió luminoterapia. Una semana después el neonato fue externado sin secuelas aparentes. Posteriormente, a raíz de una cirugía inminente y la improbabilidad de encontrar sangre compatible, se elaboró un plan para cubrir las posibles demandas. Este caso pone en evidencia la necesidad de contar a nivel nacional con un laboratorio de referencia de inmunohematología y un banco de sangre de grupos raros, que permita resolver con celeridad situaciones que requieran transfundir a estos individuos.A rare blood group is usually defined as the absence of a high prevalence antigen or the absence of several antigens within a single blood group system. These individuals may develop clinically significant red cell antibodies to the high incidence red cell antigens they lack. A 33-year-old alloimmunized woman was referred to our center at the 12th week of her third pregnancy for evaluation and follow up. The laboratory

  14. Distribution of ABO, Rh, MNSs, Kell and Duffy blood-group antigens in population of Vojvodina

    Directory of Open Access Journals (Sweden)

    Vojvodić Svetlana

    2003-01-01

    Full Text Available Introduction Analysis of erythrocyte blood group antigen polymorphisms and genetic variability in population of Vojvodina was performed by investigating gene and genotype frequencies which determine antigens of ABO, Rh, MNSs, Kell and Duffy blood-group systems. Material and methods We investigated 350 unrelated persons from Vojvodina in regard to appurtenance of ABO, Rh, MNSs, Kell and Duffy blood-group systems. We calculated gene, genotype, phenotype frequencies and proportion significance test. Results and discussion Results of investigation revealed that gene and genotype frequencies of investigated blood-group systems are similar to corresponding data for majority of European populations, while statistically significant differences were established in inhabitants of geographically distant regions. Values of proportion significance test revealed statistically significant differences of genotype frequencies for ABO and MNSs blood-group antigens in populations of: Australian Aborigines, Chinese population, Arabians, Blacks, Eskimos, American Indians (Navaho and Pueblo and population of Papua New Guinea. Statistically significant differences of genotype frequencies were established in inhabitants of narrow geographical areas of Europe such as: Finland, Germany, Sweden, Albania, England and Netherlands. Conclusion Our results point to the fact that erythrocyte blood-groups have different frequencies in some parts of the world, and that there are great differences in frequencies of some blood-groups among inhabitants of various continents and races. Genetical peculiarity of the population of Vojvodina points to the fact that differences in blood-group frequencies are also present among inhabitants of narrow geographical areas.

  15. Specificity and kinetics of norovirus binding to magnetic bead- conjugated histo-blood group antigens

    Science.gov (United States)

    Histo-blood group antigens (HBGA) have been identified as candidate receptors for human norovirus (NOR). Type A, type H1, and Lewis histo-blood group antigens (HBGAs) in humans have been identified as major targets for NOR binding. Pig HBGA-conjugated magnetic beads have been utilized as a means ...

  16. 21 CFR 864.9160 - Blood group substances of nonhuman origin for in vitro diagnostic use.

    Science.gov (United States)

    2010-04-01

    ... vitro diagnostic use. 864.9160 Section 864.9160 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT... nonhuman origin for in vitro diagnostic use. (a) Identification. Blood group substances of nonhuman origin for in vitro diagnostic use are materials, such as blood group specific substances prepared...

  17. EFFICACY OF ACUPUNCTURE THERAPY ON A, B AND O BLOOD GROUPS--Relationship between A, B, O blood groups and the acupuncture therapy

    Institute of Scientific and Technical Information of China (English)

    Amit Kr. Chakraborty; Mrigendranath Gantait; Biswapati Mukherjee

    2005-01-01

    Efficacy of acupuncture therapy varies in patients with similar ailments. The present study was undertaken to search for a marker for better efficacy of acupuncture therapy. The study was made in 224 patients including osteoarthritis 141 (62.94 %), polyarthritis 23 (10.26 %), Bursitis & synovitis 15 (6.69 %) and others 45 (20.08 %). ABO blood groups were tested for each patient. It appears that patients belonging to group AB and B responded well to acupuncture therapy in proportionately more number. Good result was achieved in 47.82 % cases in group AB and 46.04 % cases in group B, whereas patients of group A and O showed good result in 27.65 % and 26.15 % cases respectively. Apparently it may be concluded that patients of AB & B blood groups would respond comparatively well to acupuncture therapy.

  18. A STUDY OF DISTRIBUTION OF ABO AND RH BLOOD GROUPS SYSTEM AMONG BLOOD DONORS AT A TERTIARY CARE HOSPITAL

    Directory of Open Access Journals (Sweden)

    Rajesh Kumar

    2015-02-01

    Full Text Available Up till now about 400red cells antigen have been identified. The majority are inherited by Mendelian fashion. The ABO and Rh blood group system was first to be identified and is most important for blood transfusion purposes. OBJECTIVE: This study was conducted to determine the frequency of ABO and Rhesus (Rh blood groups in a tertiary care teaching hospital in India. MATERIALS AND METHODS: A retrospective data based study was conducted at blood bank , Chirayu Medical College and Hospital, Bhopal, Madhya Pradesh, India over a period of four years. RESULTS: Study includes a record of 3188 (28.54% voluntary and 7982 (71.46% replacement donors attending blood bank from February 2011 to January 2015. Out of 11170, 10723(95.998% were male and 447(4.002% female donors. The most common blood group was found to be B in 4013 (35.927% donors followed by O in 3462 (30.994% donors , an in 2516 (22.524% donors and AB in 1179 (10.555% donors. Out of these, 10659(95.425% donors were Rh - positive while 511 ( 4.575 % were Rh - negative.

  19. Emergency dilatation and curettage in a patient with Bombay blood group.

    Science.gov (United States)

    Ali, Muhammad Asghar; Sohaib, Muhammad

    2014-08-01

    Bombay blood group is a rare autosomal recessive phenotype within the ABO blood group. It represents genetically suppressed A, B and H genes. When considering such patients for transfusion, only blood of identical Bombay type can be safely transfused. We are reporting a patient having Bombay phenotypic blood, underwent emergency dilatation and curettage with active per vaginal bleeding due to retained products of placenta. There are numerous anaesthetic considerations, including emergency surgery with hemodynamic instability due to ongoing blood loss, dilutional coagulopathy as well as presence of Bombay phenotype that severely limit the possibility of red blood cell transfusion. Only four donors were registered with the blood bank of the institution and none was traceable. It becomes a real challenge for the anesthesiologist to manage such type of patients without having units of red packed cell which management is described hereby.

  20. Personality traits of aggression-submissiveness and perfectionism associate with ABO blood groups through catecholamine activities.

    Science.gov (United States)

    Hobgood, Donna K

    2011-08-01

    Personality trait research has shown associations with many genes, prominently those of the catecholamine metabolism such as dopamine beta hydroxylase (DBH), catechol-O-methyltransferase (COMT), and monoamine oxidase A (MAOA). Because DBH gene is in linkage disequilibrium with ABO gene, there is reason to think that other catecholamine genes using the same substrate as DBH may also have associations with ABO blood groups, and this paper demonstrates how this may be so. Reasons include similarities in hapmap population frequency distributions, similarities in illness risks between ABO blood groups and DBH activities as well as between ABO blood groups and COMT activities and between ABO blood groups and MAOA activities. If ABO blood groups can be demonstrated to associate with all these catecholamine genes, then the catecholamine personality trait research can be applied to ABO blood groups and tested for confirmation. ABO blood typing is widely available and affords ability to test this hypothesis and thus confirm the possible joint association of personality traits of aggression-submissiveness and perfectionism to catecholamine genes and to ABO blood groups. Clinical applications and implications are discussed. PMID:21601990

  1. Relationship between Duffy blood groups genotypes and malaria infection in different ethnic groups of Choco- Colombia

    Directory of Open Access Journals (Sweden)

    Gonzalez, Lina

    2012-09-01

    Full Text Available Background: The negative homozygous condition for the Duffy blood group (Fy-/Fy- confers natural resistance to Plasmodium vivax infection. In this direction, studies carried out in Colombia are scarce.Objective: To describe the relationship between Duffy genotypes in three ethnic communities in La Italia (Chocó and malaria infection.Methodology: a descriptive, cross-sectional study in symptomatic and asymptomatic malaria subjects. Sample size : AfroAmerican, 73; Amerindian (Emberá, 74 and Mestizo, 171. Presence of Plasmodium infection was assessed by thick smear and the status of the Duffy gene by PCR and RFLP in order to identify the substitutions T-46C y A131G which origin the genotypes T/T, T/C , C/C y G/G, G/A, A/A.Results: Infection by Plasmodium was detected in 17% with 62% due to P. falciparum and 27% to P. vivax. Duffy genotypes were significantly associated to ethnicity (p=0,003. Individuals with the C/C, A/A diplotype were exclusively infected by P. falciparum, whereas other diplotypes were infected with either species. In the Amerindian and Mestizo populations, the frequency of the T-46 allele was 0,90-1,00, among Afrocolombians this was 0,50, equal to the C allele and with absence of heterozygous At locus 131, the highest frequency of the G allele was 0,30 in Amerindians and the A allele was 0,69 in Afrocolombians. Conclusions: In the Amerindian and mestizo populations studied, a predominance of the allele T-46 (FY+ was observed, but P. vivax was not the most common. Infection by P. vivax was out ruled in all FY- individuals.

  2. Study on serological blood group conversion rule and clinical blood transfusion in allogeneic hematopoietic stem cell transplantation%异基因造血干细胞移植血型血清学转换规律与临床输血研究

    Institute of Scientific and Technical Information of China (English)

    余忠清; 高志峰; 李慧玉

    2012-01-01

    目的 探讨异基因造血干细胞移植(allo-HSCT)血型血清学和血型物质转换规律,为临床特殊血型鉴定和输血提供理论基础.方法 HSCT后动态观察受者白细胞和红细胞生命周期,红细胞嵌合状态与完全转型后血型抗体生成与残留以及血型物质的转换规律,用盐水介质试管法和微柱凝胶法正、反定型,免疫抑制法检测血型物质.结果 21例受者造血干细胞植活平均时间为18.6 d.8例主侧血型不合红细胞生长为56.6 d,9例次侧血型不合为25.9 d,4例主、次侧血型均不合为67 d(P0.01).%Objective To explore the conversion rule of serological blood group and blood group substance after successful allogeneic hematopoietic stem cell transplantation, and to provide theory for clinical special blood type identification and blood transfusion. Methods The growth cycle of recipient WBC and RBC, RBC chimera, blood group antibody production and remaining in full transition were observed. Conversion rule of blood group substance, contradiction between cells typing and sera typing were detected by saline medium tube method and microcolumn gel method after stem cells transplantation. Results The average time of engraftment in 21 recipients was about 18.6 days, RBC growth cycle in 8 major blood type incompatibility was 56.6days, 25.9 days in 9 minor blood type incompatibility, 67 days in 4 bidirectional blood type incompatibility (P0.01).

  3. Clinically Significant Minor Blood Group Antigens amongst North Indian Donor Population

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    Divjot Singh Lamba

    2013-01-01

    Full Text Available Background. Racial differences in blood group antigen distribution are common and may result in striking and interesting findings. These differences in blood group antigen distribution are important due to their influence on the clinical practice of transfusion medicine. Study Design and Methods. This is a prospective study, involving 1000 healthy regular repeat voluntary blood donors associated with the department. The clinically significant minor blood group antigens of these donors were studied. Results. Out of 1000 healthy regular repeat voluntary blood donors, 93% were D positive and 2.8% were K positive. Amongst the Rh antigens, e was the most common (99%, followed by D (93%, C (85.1%, c (62.3%, and E (21.5%. Within the MNS blood group system, antigen frequency was M (88%, N (57.5%, S (57.8%, and s (87.5%. Within the Duffy blood group system, antigen frequency was Fya (87.3% and Fyb (58.3%. Conclusions. This data base will help us to prevent alloimmunisation in young females, pregnant women, and patients who are expected to require repeated transfusions in life by providing them with antigen matched blood. Antigen negative blood can also be made available without delay to already alloimmunized multitransfused patients.

  4. A STUDY TO FIND CORRELATION BETWEEN DERMATOGLYPHIC PATTERNS AND ABO BLOOD GROUPS

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    Usha Verma

    2015-09-01

    Full Text Available Background: Dermatoglyphics, the study of fingerprints are constant and individualistic. It has been found useful in forensic medicine and identification purpose. It is useful in medical diagnosis of genetically inherited diseases and in detection of crimes. Objectives: The present study was conducted to correlate between digital dermatoglyphics patterns in ABO, Rh blood groups and to evaluate their significance. Methods: A total of 200 first year MBBS students of Pt. B.D. Sharma PGIMS, Rohtak with known blood groups from age group 17-22 yrs were included in the study. Fingerprints were obtained by Ink method. Parameters studied were arches, whorls, loops. Results: Majority of the subjects (43.5% in the study were of blood group A followed by blood group O, A and AB of whom 94.5% were Rh-positive. The general distribution of pattern of finger print showed high frequency (51.87% of loops followed by whorls and arches. Almost same order was noticed in both Rh-positive and Rh-negative individuals or A, B, AB and O blood groups, except blood group O-ve which showed more whorls. Conclusion: There is an association between distribution of finger print pattern and blood groups.

  5. DISTRIBUTION OF CLASSICAL ABO BLOOD GROUPS AMONG TYPE 2 DIABETES MELLITUS PATIENTS : AN ANALYSIS

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    Rama Devi

    2015-04-01

    Full Text Available BACKGROUND: At present Diabetes Mellitus is a global phenomenon with the disease topping the list, comprising of about 32 million cases , India is in the forefront with 30% of the cases . The disease affects multiple organs and is a leading cause of much morbidity and mortality. Since it is a multi - factorial disease a major step would be to identify different associated factors, for an early diagnosis and prompt treatment. The ABO blood groups are often associated with several diseases, with one blood group more often seen with the patients of a particular disease. Our study will help to determine the frequency and distribution of blood groups in correlation with Diabetes Mellitus. MATERIAL & METHODS: This study was conducted in the Gandhi Medical College, Secunderabad, during a two year period. A random study involving every third diabetic patient was chosen and their blood group was determined. A total of 3 00 patients were selected with 150 male and 150 female patients. Another 300 volunteers who were not diabetics were chosen as controls and their blood groups were also determined. A pro - forma was given to both diabetics and controls which included the following variables : 1 . Demographic data 2. Blood grouping 3. Fasting and post prandial blood sugar. Following this, blood groups of both cohorts and controls were determined by antigen antibody agglutination method. Data analysis was do ne after data was entered into excel sheet and double checked for errors using SPSS Software RESULTS: Our a nalysis showed that O group was significantly more among diabetic patients when all patients were compared to control . ² there was a preponderance of blood group O among female diabetics and B among male diabetics. CONCLUSION: ABO blood groups have been determined in 300 diabetic patients and compared with the controls comprising of a series of 300 voluntary blood donors. When the results were analysed on the basis of sex, there was preponderance

  6. Frequencies of red blood cell major blood group antigens and phenotypes in the Chinese Han population from Mainland China.

    Science.gov (United States)

    Yu, Y; Ma, C; Sun, X; Guan, X; Zhang, X; Saldanha, J; Chen, L; Wang, D

    2016-08-01

    Alloantibodies directed to red blood cell (RBC) antigens play an important role in alloimmune-mediated haemolytic transfusion reactions and haemolytic disease of the foetus and newborn. The frequencies and phenotypes of RBC antigens are different in populations from different geographic areas and races. However, the data on major blood group antigens in the Chinese Han population from Mainland China are still very limited; thus, we aimed to investigate them in this study. A total of 1412 unrelated voluntary Chinese Han blood donors were randomly recruited. All donors were typed for blood group antigens: D, C, c, E, e, C(w) , Jk(a) , Jk(b) ,M, N, S, s, Le(a) , Le(b) , K, k. Kp(a) , Kp(b) , Fy(a) , Fy(b) , Lu(a) , Lu(b) , P1 and Di(a) using serological technology. Calculations of antigen and phenotype frequencies were expressed as percentages and for allele frequencies under the standard assumption of Hardy-Weinberg equilibrium. Amongst the Rh antigens, D was the most common (98.94%) followed by e (92.28%), C (88.81%), c (58.43%), E (50.78%) and C(w) (0.07%) with DCe/DCe (R1 R1 , 40.72%) being the most common phenotype. In the Kell blood group system, k was present in 100% of the donors and a rare phenotype, Kp (a+b+), was found in 0.28% of the donors. For the Kidd and Duffy blood group systems, Jk (a+b+) and Fy (a+b-) were the most common phenotypes (44.05% and 84.35%, respectively). In the MNS blood group system, M+N+S-s+ (45.54%) was the most common, whereas M+N-S-s- and M-N+S-s- were not found. The rare Lu (a-b-) and Lu (a+b+) phenotypes were identified in 0.43% and 1.13% of the donors, respectively. Le(a) and Le(b) were seen in 17.92% and 63.03% of donors, respectively. The frequency of Di(a) was 4.75%, which was higher than in the Chinese population in Taiwan region or the Caucasian and Black populations (P < 0.0001). This study systematically describes the frequencies of 24 blood group antigens in the Chinese Han population from Mainland China. The data can

  7. Determination of ABO blood grouping and Rhesus factor from tooth material

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    Pooja Vijay Kumar

    2016-01-01

    Conclusion: In dentin and pulp, the antigens of ABO and Rh factor were detected up to 12 months but showed a progressive decrease in the antigenicity as the time period increased. When compared the results obtained of dentin and pulp in ABO and Rh factor grouping showed similar results with no statistical significance. The sensitivity of ABO blood grouping was better than Rh factor blood grouping and showed a statistically significant result.

  8. Breast cancer incidence in Greek women in relation to ABO blood groups and Rh factor

    OpenAIRE

    Stamatakos, Michael; Kontzoglou, Konstantinos; Safioleas, Panagiotis; Safioleas, Constnatinos; Manti, Christina; Safioleas, Michael

    2009-01-01

    Aim To investigate the correlation between breast cancer in Greek women and ABO blood groups. Material-methods In 166 female patients with breast cancer factors such as blood group, histological type, family history, presence or absence of nodal and/or distant metastases were examined. These patients had similar demographic, clinical, surgical, immunohistochemical, laboratory, and follow-up data and this group is representative of general population of women in Greece. Results The ductal type...

  9. Phenotypic and allelic distribution of the ABO and Rhesus (D) blood groups in the Cameroonian population.

    Science.gov (United States)

    Ndoula, S T; Noubiap, J J N; Nansseu, J R N; Wonkam, A

    2014-06-01

    Data on blood group phenotypes are important for blood transfusion programs, for disease association and population genetics studies. This study aimed at reporting the phenotypic and allelic distribution of ABO and Rhesus (Rh) groups in various ethnolinguistic groups in the Cameroonians. We obtained ABO and Rhesus blood groups and self-identified ethnicity from 14,546 Cameroonian students. Ethnicity was classified in seven major ethnolinguistic groups: Afro-Asiatic, Nilo-Saharan, Niger-Kordofanian/West Atlantic, Niger-Kordofanian/Adamawa-Ubangui, Niger-Kordofanian/Benue-Congo/Bantu/Grassfield, Niger-Kordofanian/Benue-Congo/Bantu/Mbam and Niger-Kordofanian/Benue-Congo/Bantu/Equatorial. ABO allelic frequencies were determined using the Bernstein method. Differences in phenotypic distribution of blood groups were assessed using the chi-square test; a P value blood groups O, A, B and AB were 48.62%, 25.07%, 21.86% and 4.45%, respectively. Rhesus-positive was 96.32%. The allelic frequencies of O, A and B genes were 0.6978, 0.1605 and 0.1416, respectively. Phenotypic frequencies of the blood groups in the general study population and in the different ethnolinguistic groups were in agreement with Hardy-Weinberg equilibrium expectations (P > 0.05). The frequencies of O, A, and B blood phenotypes were significantly lower, respectively, in the Nilo-Saharan group (P = 0.009), the Niger-Kordofanian/Benue-Congo/Bantu groups (P = 0.021) and the Niger-Kordofanian/West-Atlantic group. AB blood group was most frequent in the Niger-Kordofanian/Adamawa-Ubangui group (P = 0.024). Our study provides the first data on ethnic distribution of ABO and Rhesus blood groups in the Cameroonian population and suggests that its general profile is similar to those of several sub-Saharan African populations. We found some significant differences in phenotypic distribution amongst major ethnolinguistic groups. These data may be important for blood donor recruitment policy and blood transfusion

  10. The prevalence of transfusion transmitted infections in ABO blood groups and Rh type system

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    Jitendra Singh Nigam

    2014-12-01

    Full Text Available Screening of blood and blood products is important to reduce the risk of transfusion transmitted infections (TTIs. The transfusion of unscreened or inadequately screened blood and blood products are the major source of TTIs. The aim of this paper is to find out the prevalence of TTIs in ABO blood groups and Rh type system. A total of 4128 blood donors were screened from January 2010 to April 2014. Serological tests were performed for hepatitis B surface antigen (HBsAg, anti hepatitis C virus (Anti-HCV, anti HIV-1 and 2, venereal disease research laboratory test (VDRL and malaria parasite (MP antigen. In seroreactive donors, HBsAg, Anti-HCV, VDRL, MP antigen and anti HIV were positive in 40 cases, 26 cases, 19 cases, 6 cases and 2 cases, respectively. Highest percentage of HBsAg, Anti HCV, VDRL, MP antigen and anti HIV was observed in blood group A negative (2/50, O negative (1/66, B negative (1/91, AB positive (2/377 blood group respectively. In the present study, the total number of Rh-negative donors is lower when compared to Rh-positive blood donors, but Rh-negative blood donors show higher percentages of seroreactivity for TTIs. Larger scale studies at molecular level are required to improve the knowledge of this aspect.

  11. Prognostic Impact of ABO Blood Group on the Survival in Patients with Ovarian Cancer

    OpenAIRE

    Zhou, Juan; Yang, Li-Chao; He, Zhen-Yu; Li, Fang-Yan; Wu, San-Gang; Sun, Jia-yuan

    2015-01-01

    Purpose: The impact of ABO blood group on the survival of patients with ovarian cancer remains uncertain. The aim of this study was to evaluate the prognostic value of the ABO blood group in ovarian cancer patients. Methods: 256 ovarian cancer patients who received a cytoreductive surgery were retrospectively reviewed. The prognostic impact of the ABO blood group with respect to overall survival (OS) was analyzed. Results: The median follow-up time was 57 months and the 5-year OS was 70.1%. T...

  12. The distribution of blood group antigens in experimentally produced carcinomas of rat palate

    DEFF Research Database (Denmark)

    Reibel, J; Philipsen, H P; Fisker, A V;

    1986-01-01

    It has been shown previously that rat oral epithelia express antigens cross-reacting with antibodies against human blood group antigen B and its structural precursor, the H antigen (Type 2 chain). In the present study we investigated the expression of these antigens in malignant changes in the rat....... The blood group antigen staining pattern in experimentally produced verrucous carcinomas showed an almost normal blood group antigen expression. This may have diagnostic significance. Localized areas of hyperplastic palatal epithelium with slight dysplasia revealed loss of H antigen and the presence of B...

  13. Rapid method for identification of group B streptococci in neonatal blood cultures.

    OpenAIRE

    Holmes, R. L.; Harada, W A

    1981-01-01

    A rapid technique used for the identification of Streptococcus agalactiae, Lancefield group B, from the blood cultures of two neonatal infants is reported. The method utilized the Phadebact Streptococcus Test System (Pharmacia Diagnostics, Piscataway, N.J.) and the supernatant from 13- and 14-h blood cultures. Additional studies with simulated neonatal blood cultures revealed that this method was reproducible. Additional studies also revealed that some non-specific agglutination did occur, wh...

  14. Prognostic value of ABO blood group in patients with surgically resected colon cancer

    OpenAIRE

    X. Cao; Wen, Z-S; Sun, Y-J; Li, Y.; Zhang, L.; Han, Y-J

    2014-01-01

    Background: Previous studies supported a link between the ABO blood type and survival for several types of malignancies. Nonetheless, the relationship between ABO blood type and survival in colon cancer patients has not been rigorously evaluated. The goal of this retrospective analysis was to discern the correlations between ABO blood group and colon cancer survival. Methods: A total of 1555 colon cancer patients that underwent curative-intent surgery between October 1995 and June 2002 were e...

  15. ABO-incompatible kidney transplantation

    DEFF Research Database (Denmark)

    Schousboe, Karoline; Titlestad, Kjell; Baudier, Francois;

    2010-01-01

    INTRODUCTION: Kidney transplantation is the optimal treatment for many patients with end-stage renal disease (ESRD). Due to shortage of donor kidneys in Denmark, there is a need to expand the possibilities for donation. At the Odense University Hospital (OUH), we have introduced ABO-incompatible ......INTRODUCTION: Kidney transplantation is the optimal treatment for many patients with end-stage renal disease (ESRD). Due to shortage of donor kidneys in Denmark, there is a need to expand the possibilities for donation. At the Odense University Hospital (OUH), we have introduced ABO......-cell-mediated rejection, and one patient died of myocardial infarction with a functioning graft on the third post-operative day. Both rejections were treated effectively. Among the patients, the average serum creatinine level was 128 micromol/l. CONCLUSION: The rejection and mortality rates for ABO-incompatible kidney...

  16. The use of traditional Chinese herb medicine in the treatment of maternal-fetal blood group incompatibility: attached a case report

    Institute of Scientific and Technical Information of China (English)

    徐蕴华; 盖铭英; 刘俊涛; 杨剑秋; 刘善英; 边旭明; 崔子琴; 葛秦生

    2003-01-01

    @@ Patient,Hosp No.C-690021,female,age 34,G3/P2 .LMP:Jan.4,2000.EDC:Oct.11,2000,Menses 7/30-32 days.She wasreferred to our hospital from Xiamen because of her third pregnancy with fetal ascites at 25 weeks.

  17. Bell inequalities and incompatible measurements

    OpenAIRE

    Morgan, Peter

    2003-01-01

    Bell inequalities are a consequence of measurement incompatibility (not, as generally thought, of nonlocality). In classical terms, this is equivalent to contextuality -- measurement devices do have a significant effect. Contextual models are reasonable in classical physics, which always took the view that we ignore measurement devices whenever possible, but if that isn't good enough then we do have to model measurement devices. It is also argued that quantum theory should only be taken with ...

  18. Relationship between ABO blood groups and malaria with clinical outcome in rural area of South India

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    Gayathri B.N.

    2013-05-01

    Full Text Available Background A number of studies have shown that susceptibility to several infectious diseases is related to the patient’s blood group. Although the relationship between blood group and susceptibility to malaria has been studied by several researchers, the results have been contradictory. Since malaria has re-emerged as a major problem in India during the past few years, it would be useful to know whether there is any relationship between blood group and infection. Objectives The study will be undertaken to correlate the blood groups and clinical presentations in malaria patients and to understand the differential host susceptibility in malaria. Method Over a period of 4 years malaria positive samples identified by peripheral smear (thin and thick smears will be evaluated in this study. Haemoglobin, total leucocyte count, differential leucocyte count and platelet count of each patient done on an automated cell counter will be retrieved from the data. Blood group was determined by forward and reverse method. The demographic details of the patients and clinical details were obtained from case records of the patients. Malarial species and the severity of clinical course were correlated with blood groups Results A total of 205 patients were included in the study, of which 123 cases were positive for plasmodium falciparum and 78 cases were positive for P. vivax infection and 4 patients had mixed infection. The results of blood groups showed 33 -‘A’ group, 84 -‘B’ group, 70 -‘O’ group and 18 were ‘AB’ group. When the clinical courses between different groups were compared using the following parameters for severe infection- a parasitic load of > 10/1000 RBCs, severe anemia with haemoglobin 101o F and the other organ involvement, it was observed that there was no significant relationship between ABO blood group and malaria in our population, this could be due to various demographic reasons. Conclusions The present study indicate that

  19. Assessing the association of severe malaria infection and ABO blood groups in northwestern Ethiopia

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    Hailu Tadesse

    2013-12-01

    Full Text Available Background & objectives: There is lack of adequate information on the association between severe malaria and some human genetic markers like ABO blood types. The study was undertaken to evaluate the association between severe malaria infection and ABO blood types among febrile patients attending Felegeselam Health Center, northwestern Ethiopia. Methods: A total of 398 febrile patients were examined for malaria and tested for ABO blood groups in December 2011. The blood samples were collected by finger pricking, stained with Giemsa and slides were examined microscopically. ABO blood group was determined by agglutination test using agglutinating A and B monoclonal anti-sera together with parasite load count. Chi-square and ANOVA tests were used to assess the difference between frequencies and means, respectively. Results: Out of 398 acute febrile patients, 201 (50.5% were found to be infected with Plasmodium parasites. Of which 194 (48.74% and 7 (1.76% belong to Plasmodium falciparum and P. vivax, respectively. The distribution of ABO blood groups was O (46%, A (27.1%, B (23.1% and AB (3.8%. The percentage of severe malaria with respect to blood group A, B, AB and O was found to be 40, 34.1, 14.3 and 5.1%, respectively. The association of severe malaria with non 'O' blood types was statistically significant (χ2 = 31.246, p <0.01. Interpretation & conclusion: The present findings indicate that individuals with blood groups A, B and AB are more susceptible for severe malaria infection than blood group O.

  20. [Heart surgery in a female patient with blood group Oh (Bombay phenotype)].

    Science.gov (United States)

    Schricker, K T; Neidhardt, B; Hacker, R; Kail, R

    1983-01-14

    A 62-year-old woman with stenosing coronary artery disease had the rare blood group Oh (Bombay phenotype). After prophylactic deep-freeze conservation of autologous blood, direct myocardial revascularization was successfully accomplished under extracorporeal circulation. Three deep-freeze units of erythrocyte concentrates were used. Both operation and postoperative wound healing progressed without complication.

  1. ABO Blood Group and Dementia Risk--A Scandinavian Record-Linkage Study

    DEFF Research Database (Denmark)

    Vasan, Senthil K; Rostgaard, Klaus; Ullum, Henrik;

    2015-01-01

    BACKGROUND: Dementia includes a group of neuro-degenerative disorders characterized by varying degrees of cognitive impairment. Recent data indicates that blood group AB is associated with impaired cognition in elderly patients. To date there are no large-scale studies that have examined...... the relationship between ABO blood group and dementia-related disorders in detail. METHODS: We used data from the SCANDAT2 database that contains information on over 1.6 million blood donors from 1968 in Sweden and 1981 from Denmark. The database was linked with health outcomes data from nationwide patient...... and cause of death registers to investigate the relationship between blood groups and risk of different types of dementia. The incident rate ratios were estimated using log-linear Poisson regression models. RESULTS: Among 1,598,294 donors followed over 24 million person-years of observation we ascertained 3...

  2. AMERICAN NATIONAL RED CROSS BLOOD PROGRAM AWARD GROUP - LEFT TO RIGHT - SEATED - JOHN S BROWN - MISS

    Science.gov (United States)

    1956-01-01

    AMERICAN NATIONAL RED CROSS BLOOD PROGRAM AWARD GROUP - LEFT TO RIGHT - SEATED - JOHN S BROWN - MISS ELEANOR KIPLINGER - DR SHARP - JESSIE SHEWARD - DR VICTORY - FIRST ROW - GORDON ROMIG - ROBERT BRIGADOI - MIKE VACCARO - ALFRED VALERINO -

  3. The Purification of a Blood Group A Glycoprotein: An Affinity Chromatography Experiment.

    Science.gov (United States)

    Estelrich, J.; Pouplana, R.

    1988-01-01

    Describes a purification process through affinity chromatography necessary to obtain specific blood group glycoproteins from erythrocytic membranes. Discusses the preparation of erythrocytic membranes, extraction of glycoprotein from membranes, affinity chromatography purification, determination of glycoproteins, and results. (CW)

  4. Non-ABO blood group systems phenotyping in non-human primates for blood banking laboratory and xenotransplantation.

    Science.gov (United States)

    Ramis, G; Martínez-Alarcon, L; Quereda, J J; Mrowiec, A; Funes, C; Ríos, A; Ramírez, P; Muñoz, A; Majado, M J

    2013-04-01

    Some biomedical research procedures, such as organ xenotransplantation, usually require intensive hemotherapy. Knowledge of the whole phenotype of blood donor and graft could be useful in the field of xenotransplantation. Human and simian-type categories of blood groups have been established and they can be tested by standard methods used for human blood grouping. The aim of this work was to study the incidence of non-ABO blood group systems in different species of non-human primates, which are employed in biomedical research. The phenotype of Rh, Lewis, Kidd, Kell, MNSs, Lutheran, P and Duffy antigens was investigated in olive baboon (n = 48), chacma baboon (n = 9), Guinea baboon (n = 14), Rhesus macaque (n = 38) and squirrel monkey (n = 30) by using commercial microtyping cards. Kell, Lutheran, Kidd and Duffy antigens have been detected in all species, Rh in squirrel monkey, MNSs in rhesus macaque and squirrel monkey, and Lewis in baboon and rhesus macaque. There were differences in frequency and haemagglutination scores between species regardless of their gender and age. The main differences were found in squirrel monkey when compared with baboons and macaques. This typing system provides a tool to assess the presence of antigens in animals used for experimental procedures, such as xenotransplantation and xenotransfusion. PMID:23563364

  5. ABO Blood Group and Dementia Risk--A Scandinavian Record-Linkage Study.

    Directory of Open Access Journals (Sweden)

    Senthil K Vasan

    Full Text Available Dementia includes a group of neuro-degenerative disorders characterized by varying degrees of cognitive impairment. Recent data indicates that blood group AB is associated with impaired cognition in elderly patients. To date there are no large-scale studies that have examined the relationship between ABO blood group and dementia-related disorders in detail.We used data from the SCANDAT2 database that contains information on over 1.6 million blood donors from 1968 in Sweden and 1981 from Denmark. The database was linked with health outcomes data from nationwide patient and cause of death registers to investigate the relationship between blood groups and risk of different types of dementia. The incident rate ratios were estimated using log-linear Poisson regression models.Among 1,598,294 donors followed over 24 million person-years of observation we ascertained 3,615 cases of Alzheimer's disease, 1,842 cases of vascular dementia, and 9,091 cases of unspecified dementia. Overall, our study showed no association between ABO blood group and risk of Alzheimer's disease, vascular dementia or unspecified dementia. This was also true when analyses were restricted to donors aged 70 years or older except for a slight, but significantly decreased risk of all dementia combined in subjects with blood group A (IRR, 0.93; 95% confidence interval [CI], 0.88-0.98, compared to those with blood group O.Our results provide no evidence that ABO blood group influences the risk of dementia.

  6. [Correlation between Staphylococcus carriage, specific antibody-production and AB0-blood grouping in plasma donors].

    Science.gov (United States)

    Nemyrovs'ka, L M; Patoka, V V

    2002-01-01

    Interaction peculiarities of three components of the immune human homeostasis-antigens of blood groups AB0, staphylococcus antigens and antistaphylococcus antibodies have been investigated. Donors (85) of antistaphylococcus plasma immunized by staphylococcus anatoxin have been investigated. It is found that the nasal staphylococcus carriage in donors depends on the level of specific and natural antibodies and on the coincidence between the staphylococcus antigen structure and the protein substance of the specific blood group factors. PMID:12190026

  7. Research study of diversity of Rh and ABO blood groups in 110 psoriatic patients

    OpenAIRE

    Valieghanie M

    1996-01-01

    Psoriasis is an common, chronic, recurrent, inflammatory disease of the skin, characterized by red scaling plaques on the skin surface. The morphology of psoriatic lesions allows classification of the different types of psoriasis that included plaque type, pustular type and Erythrodermic type. I have studied the relationship between distribution of RH and ABO blood groups in 110 psoriatic patients and compared with control normal blood groups. The result of this study was as follow: The rate ...

  8. Relationship between ABO blood groups and carcinoma of esophagus and cardia in Chaoshan inhabitants of China

    Institute of Scientific and Technical Information of China (English)

    Min Su; Shan-Ming Lu; Dong-Ping Tian; Hu Zhao; Xiao-Yun Li; DeRui Li; Zhi-Chao Zheng

    2001-01-01

    AIM To study the relationship between ABO blood groups and carcinoma of esophagus and cardia in Chaoshan inhebitants of China, which is a unique Littoral high-risk area of esophageal carcinoma in China. The poor communication and transportation in the psst has made Cheoshan a relatively closed area and kept its culture and costure of old China thousend years ago.``METHODS Data on age, sex, ABO blood type and X-rayor psthological diagnose of the pstients with carcinoma of esophagus or cardia were collected from the Tumor Hospital. First Affiliated Hospital, Second Affiliated Hospital of Shantou University Medical College; and the Central Hospital of Shantou and the Central Hospital of Jieyang. A total of 6685 pstients with esophageal carcinoma (EC) and 2 955 patients with cardiac cancer (CC) in Chaoshen district were retrospectively assessed for their association with ABO blood groups.``RESULTS The distribution of ABO blood groups in patients with EC or CC was similar to the norrnal local population in Chaoshen. However, blood group B in male patients with CC and in the pstients with carcinoma in the upper third esophagus was 2.3% and 4.7% higher than the corresponding controls. The relative risk B: O was 1. 1415 (P<0.05)and 1 .2696 (P<0.05), respectively. No relationship was found between ABO blood groups and tumor differentiation.``CONCLTUSION ABO blood group B is associated with the incidence of CC in male individuals and carcinona in the upper third esophagus. The distribution of ABO blood groups varies in the different geographical and ethnic groups. As a result, proper controls are very important for such studies.``

  9. [Features of arterial blood pressure in elderly persons of different ethnic groups in Yakutsk].

    Science.gov (United States)

    Nikitin, Iu P; Tatarinova, O V; Neustroeva, V N; Shcherbakova, L V; Sidorov, A S

    2013-01-01

    The differences in arterial blood pressure in the sample of population in the age of 60 and older of different ethnic groups in Yakutsk, as well as its connection with the other cardiovascular diseases risk factors have been analyzed. It was shown that the average values of systolic and diastolic blood pressure in subsample of the Yakuts appeared to be lower than in Caucasoid gerontic persons. The average values of systolic arterial blood pressure both in the Yakuts and in the Caucasoids were detected higher than normal values in all age-dependent subgroups. The average values of diastolic blood pressure in both ethnic groups were within the limits of high normal level. From 60 to 90 years and older the decrease in systolic and diastolic arterial blood pressure was detected; it was more marked in Caucasoid gerontic persons. The average values of pulse pressure in the Yakuts and in the Caucasoids appeared to be higher than the existing standard and didn't have any differences in ethnic groups. In both ethnical subsamples, pulse pressure values increase was observed in persons of 60-89 years old and its decrease after 90. Persons with overweight, obesity, central (abdominal) obesity, dyslypoproteidemias irrespective of belonging to ethnical group were characterized as having higher levels of arterial blood pressure. Statistically significant differences in the levels of arterial blood pressure in the Yakuts and in the Caucasoids depending on hyperglycemia, smoking, the presence of burdened anamnesis, educational level, marital status was not detected.

  10. Our first experiences in applying an original method for removal of ABO-isoagglutinins in ABO-incompatible kidney recipients

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    Ignjatović Ljiljana

    2009-01-01

    Full Text Available Background/Aim. Due to improved methods for removal of ABO isoagglutinins and novel immunosuppressive protocols, short and long term outcome in blood group incompatible is similar to blood group compatible kidney transplantation. The aim of this study was to determine the efficacy of our original method for removal of ABO isoagglutinins from the blood in ABO-incompatible kidney allograft recipients. Method. Between 2006 and 2008 twelve patients were transplanted from ABO incompatible living donors. Titers of ABO isoagglutinins were 4-128 (IgG. Immunosuppressive therapy started 14 days before kidney transplantation with rituximab, followed by a triple therapy (prednisone + tacrolimus + mycophenolate mofetil and the first plasma exchange (PE procedure, in which one plasma volume was substituted with albumin and saline on day 7 before transplantation. For selective extracorporeal immunoadsorption, the removed plasma was mixed with donor blood type filtered red blood cells, centrifuged and the supernatant separated and preserved. In the next PE procedure, the removed plasma was replaced with immunoadsorbed plasma, and so on. Titers of ABO agglutinins, renal allograft function and survival were followed-up. Results. The pre-transplant treatment consisting of 1-5 PE procedures and immunosuppressive therapy resulted in target ABO agglutinins titers below 4. During a 10-24 month follow-up three patients had an early acute rejection, one patient acute rejection and hemolytic anemia, two patients surgical complications and one of them lost his graft. In the post-transplant period, the titers of ABO antibodies remained below 4. All the patients had stable kidney allograft function with mean serum creatinine ±SD of 129 ± 45 μmol/l at the end of the study. Conclusion. Our method for removal of ABO antibodies was effective in a limited series of patients and short-term follow-up.

  11. Determination of ABO blood grouping and Rhesus factor from tooth material

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    Kumar, Pooja Vijay; Vanishree, M; Anila, K; Hunasgi, Santosh; Suryadevra, Sri Sujan; Kardalkar, Swetha

    2016-01-01

    Objective: The aim of the study was to determine blood groups and Rhesus factor from dentin and pulp using absorption-elution (AE) technique in different time periods at 0, 3, 6, 9 and 12 months, respectively. Materials and Methods: A total of 150 cases, 30 patients each at 0, 3, 6, 9 and 12 months were included in the study. The samples consisted of males and females with age ranging 13–60 years. Patient's blood group was checked and was considered as “control.” The dentin and pulp of extracted teeth were tested for the presence of ABO/Rh antigen, at respective time periods by AE technique. Statistical Analysis: Data were analyzed in proportion. For comparison, Chi-square test or Fisher's exact test was used for the small sample. Results: Blood group antigens of ABO and Rh factor were detected in dentin and pulp up to 12 months. For both ABO and Rh factor, dentin and pulp showed 100% sensitivity for the samples tested at 0 month and showed a gradual decrease in the sensitivity as time period increased. The sensitivity of pulp was better than dentin for both the blood grouping systems and ABO blood group antigens were better detected than Rh antigens. Conclusion: In dentin and pulp, the antigens of ABO and Rh factor were detected up to 12 months but showed a progressive decrease in the antigenicity as the time period increased. When compared the results obtained of dentin and pulp in ABO and Rh factor grouping showed similar results with no statistical significance. The sensitivity of ABO blood grouping was better than Rh factor blood grouping and showed a statistically significant result. PMID:27721625

  12. Correlation between "ABO" blood group phenotypes and periodontal disease: Prevalence in south Kanara district, Karnataka state, India

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    Gurpur Prakash Pai

    2012-01-01

    Full Text Available Background: The correlation between certain systemic diseases and ABO blood group is a well-documented fact. The association between periodontal disease and ABO blood group is not studied in relation to a specific geographic location. Here is a study conducted on a group of patients belonging to South Kanara district of Karnataka state. Materials and Methods: A total of 750 subjects aged between 30and 38 years belonging to South Kanara district were selected on random basis. The study subjects were segregated into healthy/mild gingivitis, moderate/severe gingivitis, and periodontitis group, based on Loe and Silness index and clinical attachment loss as criteria. The study group was further categorized and graded using Ramfjord′s periodontal disease index. Blood samples were collected to identify ABO blood group. Results: Prevalence of blood group O was more in South Kanara district, followed by blood groups B and A, and the least prevalent was AB. The percentage distribution of subjects with blood groups O and AB was more in healthy/mild gingivitis group (group I and moderate/severe gingivitis group (group II, while subjects with blood groups B and A were more in periodontitis group III. There was increased prevalence of subjects with blood groups O and AB with healthy periodontium, while subjects with blood groups B and A showed inclination toward diseased periodontium. Conclusion: There is a correlation existing between periodontal disease and ABO blood group in this geographic location. This association can be due to various blood group antigens acting as receptors for infectious agents associated with periodontal disease. This broad correlation between periodontal disease and ABO blood group also points toward susceptibility ofthe subjects with certain blood groups to periodontal disease.

  13. Prevalence of feline blood groups in the Montreal area of Quebec, Canada.

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    Fosset, Fabrice T J; Blais, Marie-Claude

    2014-01-01

    The feline AB blood group system has clinical significance because type B cats have natural alloimmune anti-A antibodies which can cause isoerythrolysis of the newborn and life-threatening transfusion reactions. In the United States, the prevalence of type B blood is estimated to be 1% to 2%. This study determined the prevalence of feline AB blood groups among 207 potential blood donor cats that included 178 domestic cats, in the Montreal area of Quebec, Canada. Blood typing was performed using a standardized tube technique. Blood types AB and B were confirmed using a backtyping technique. The frequency of blood types among the studied population was as follows: 95.2% type A, 4.4% type B, and 0.48% type AB. Among domestic cats, the frequency was 94.4% for type A, 5% for type B, and 0.6% for type AB. The frequency of type B was higher than expected, which reinforces the recommendation to ensure blood compatibility of the recipient and donor before transfusion through typing and possibly cross-matching as well. PMID:24381340

  14. EVOLUTION OF VEGETATIVE INCOMPATIBILITY IN FILAMENTOUS ASCOMYCETES .1. DETERMINISTIC MODELS

    NARCIS (Netherlands)

    NAUTA, MJ; HOEKSTRA, RF

    1994-01-01

    In ascomycetes vegetative incompatibility can prevent the somatic exchange of genetic material between conspecifics. It must occur frequently in natural populations, since in all species studied many vegetative compatibility groups (VCGs) are found. Using a population-genetic approach, this paper ex

  15. Cord Blood

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    Saeed Abroun

    2014-05-01

    Full Text Available   Stem cells are naïve or master cells. This means they can transform into special 200 cell types as needed by body, and each of these cells has just one function. Stem cells are found in many parts of the human body, although some sources have richer concentrations than others. Some excellent sources of stem cells, such as bone marrow, peripheral blood, cord blood, other tissue stem cells and human embryos, which last one are controversial and their use can be illegal in some countries. Cord blood is a sample of blood taken from a newborn baby's umbilical cord. It is a rich source of stem cells, umbilical cord blood and tissue are collected from material that normally has no use following a child’s birth. Umbilical cord blood and tissue cells are rich sources of stem cells, which have been used in the treatment of over 80 diseases including leukemia, lymphoma and anemia as bone marrow stem cell potency.  The most common disease category has been leukemia. The next largest group is inherited diseases. Patients with lymphoma, myelodysplasia and severe aplastic anemia have also been successfully transplanted with cord blood. Cord blood is obtained by syringing out the placenta through the umbilical cord at the time of childbirth, after the cord has been detached from the newborn. Collecting stem cells from umbilical blood and tissue is ethical, pain-free, safe and simple. When they are needed to treat your child later in life, there will be no rejection or incompatibility issues, as the procedure will be using their own cells. In contrast, stem cells from donors do have these potential problems. By consider about cord blood potency, cord blood banks (familial or public were established. In IRAN, four cord blood banks has activity, Shariati BMT center cord blood bank, Royan familial cord blood banks, Royan public cord blood banks and Iranian Blood Transfusion Organ cord blood banks. Despite 50,000 sample which storage in these banks, but the

  16. Non-association between anti-Toxoplasma gondii antibodies and ABO blood group system

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    ACF Rodrigues

    2011-01-01

    Full Text Available Toxoplasma gondii infects humans through the gastrointestinal tract (GIT, which elicits humoral immune response with specific antibodies. The expression of the ABO blood group glycoconjugates also occurs in this same system and may influence the human susceptibility of infection by T. gondii. The aim of the present study was to investigate the association between ABO blood group phenotypes and the presence of anti-T. gondii antibodies. Data - including age, results of serology tests for T. gondii infection and ABO blood group phenotypes - were assembled from the medical records of 1,006 pregnant women attended in the Base Hospital of the Medical School of São José do Rio Preto, Brazil, between 2001 and 2004. The chi-square test was used to compare the results with the level of significance set at 5%. Of the studied cases, 64.1% (645/1006 and 35.9% (391/1006 presented respectively positive and negative serology tests for anti-T. gondii antibodies. The mean age of those who tested positive was higher than those with negative serology tests (p = 0.0004. The frequencies of ABO blood group phenotypes were similar in those with and without anti-T. gondii antibodies (p = 0.35. In conclusion, the ABO blood group system is not associated with the presence or absence of anti-T. gondii antibodies.

  17. Correlation of lip prints with gender, ABO blood groups and intercommissural distance

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    Pradhuman Verma

    2013-01-01

    Full Text Available Background: In forensics, the mouth allows for a myriad of possibilities. Lip print on glass or cigarette butt found at crime scenes may link to a suspect. Hence, a dentist has to actively play his role in personal identification and criminal investigation. Aims: To investigate the uniqueness of the lip print patterns in relation to gender, ABO blood groups and intercommissural distance (ICD. Materials and Methods: The study was conducted on 208 randomly selected students. The lip print of each subject was obtained and pattern was analyzed according to Tsuchihashi classification. The blood group and ICD at rest position was recorded for each. Results: The study showed that Type II (branched lip pattern to be most prominent. The B+ blood group was the most common in both genders and the ICD is higher in males. The lip print pattern does not show any correlation between ABO blood groups, gender, and ICD. Conclusions: The lip print pattern shows no correlation with gender, ABO blood groups, or ICD. Further studies with larger samples are required to obtain statistical significance of this correlation.

  18. [A large-scale survey for rare blood group screening among blood donors in Chinese over Nanjing area].

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    Ma, Ling; Liu, Yan-Chun; Xue, Min; Wei, Peng; Tang, Rong-Cai

    2011-02-01

    The purpose of this study was to investigate the distribution of 10 rare red blood groups in Chinese Nanjing population, so as to provide compatible rare blood to patients and to create a donor data bank. Jk (a-b-) (Kidd) phenotypes were detected by urea, while H-(H), GPA-(MNS), GPC-(Gerbich), i+ (Ii) and Lub-(Lutheran) phenotypes were detected by monoclonal, polyclonal antibodies with U type 96 well microplate technology. The screening of Jsb- and k-(Kell), Fya-(Duffy), Ok-(Ok), s-(MNS) and Dib-(Digeo) phenotypes were performed by polymerase chain reaction. The results showed that 2 Jk (a-b-) out of 40337 donation samples and 3 Fy (a-b+) out of 1782 donation samples were found, while no other rare blood phenotypes (H-, GPA-, GPC-, Lub-, Ok-, s-, Jsb-, k-, Dib- and i+) were detected. It is concluded that the frequencies of Jk (a-b-) and Fya(a-b+) are 0.0049% and 0.168% respectively. No more rare blood phenotype was found in this screening.

  19. Immunochemical studies on blood groups LXII. Fractionation of hog and human A, H, and AH blood group active substance on insoluble immunoadsorbents of Dolichos and Lotus lectins.

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    Pereira, M E; Kabat, E A

    1976-02-01

    The purified lectins from Lotus tetragonolobus and Dolichos biflorus were coupled to Sepharose 2B to make insoluble adsorbents for purification and fractionation of blood group A and H active glycoproteins. With both adsorbents, hog gastric mucin A + H blood substance (HGM), purified by phenol-ethanol precipitation, yielded fractions showing only A, only H, or AH activities. The AH fraction was obtained when the adsorbent column was overloaded with HGM and its A and H specificities seem to be carried on the same molecules since they were not separable by chromatography on either column. However A and H specificities of blood group substance from the stomach of a presumably heterozygous individual hog were both on the same molecules as they too could not be fractionated on either column. Analytical properties of the isolated fractions were generally similar to those of the unfractionated material, the purfied A substances had a higher galactosamine/fucose ratio than did the H substances. Although the original A + H showed very little specific optical rotation, the separated A and H substances rotated positively and negatively, respectively. The lectin-Sepharose adsorbents have also proven useful in isolating A or H substances directly from the crude commercial hog gastric mucin. Blood group A2 substance from a human ovarian cyst yielded two fractions on the Lotus-Sepharose column; the effluent did not interact with the Lotus lectin but precipitated the Ulex and Dolichos lectins and anti-A, and appears to contain type 1 H determinants. The other fraction reacted with Lotus and Ulex lectin as well as with Dolichos and anti-A.

  20. Polycystic ovary syndrome, blood group & diet: A correlative study in South Indian females

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    Rahul Pal, Pratik Kumar Chatterjee, Poulomi Chatterjee, Vinodini NA, PrasannaMithra, Sourjya Banerjee, Suman VB2, Sheila R. Pai

    2014-07-01

    Full Text Available Aim: To find out the co-relation between polycystic ovary syndrome (PCOS with blood group & diet in South Indian females, between the age-group of (20-30 years. Objectives: Correlative analysis of ABO & Rh system, dietary habits & alcohol consumption with PCOS. Materials & Methods: 100 patients between (20-30 years, diagnosed with PCOS were selected. A standard PCOS questionnaire was given. Blood group & dietary status data were collected. Patients were grouped according to ABO & Rh system considering their diet & alcohol intake (p≤0.05 significant. Result: Our data revealed that the highest risk of PCOS was observed in females with blood group ‘O’ positive followed by ‘B’ positive who were on mixed diet & used to consume alcohol. Our study also suggests that Rh negative individuals didn’t show any association with PCOS. Conclusion: The results of our study suggest that ‘O’ positive females, are more prone to PCOS. Though the relative frequency of B positive individuals are more in India, females with blood group O positive are more susceptible to PCOS, contributing factors being mixed diet & alcohol intake. So, early screening of ‘O’ positive &‘B’ positive females of reproductive age-group in South-India, could be used as a measure for timely diagnosis of PCOS, better management &also prevention of complications. However, further research should be done to investigate the multifaceted mechanisms triggering these effects.

  1. Detection of rare blood group, Bombay (Oh phenotype patients and management by acute normovolemic hemodilution

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    Manisha Shrivastava

    2015-01-01

    Full Text Available Background: Due to lack of correct blood grouping practices, the rare Bombay Oh phenotype may be missed, subjecting patients to the risk of severe hemolytic transfusion reaction. In the absence of blood donor registry, transfusion management of patients needing immediate surgery is a challenge. This study presents detection of rare Bombay Oh phenotype patients and their management by acute peri-operative acute normovolemic hemodilution (ANH in a hospital from central India. Materials and Methods: Blood grouping of patients and blood donors with a standard tube method was carried out and samples identified as rare Bombay phenotype were confirmed by saliva inhibition test. Surgical management of cases needing transfusion was done by ANH, as per the British Committee for Standards in Hematology guidelines. Results: The incidence of Bombay phenotype was 0.002% or 1 in 51,924 in the study. Amongst three cases (patients identified as Bombay phenotype, one was Bombay Oh, Rh negative. Two cases were missed in the first instance and one case actually did not require transfusion. In the absence of a blood donor registry for Bombay phenotype, the cases needing transfusion were successfully managed with ANH in the operation theatre. Conclusion: A simple test like blood grouping should be done with serious intention with incorporation of both forward and reverse grouping, so that no patient receives wrong blood leading to fatal hemolysis due to transfusion. ANH is a cost-effective transfusion option for suitable patients. Appropriate clinical decision making, use of strategies to decrease peri-operative blood losses and cost-effective country based planning could be more widely applied to improve clinical transfusion practice.

  2. Current techniques for AB0-incompatible living donor liver transplantation

    Science.gov (United States)

    Rummler, Silke; Bauschke, Astrid; Bärthel, Erik; Jütte, Heike; Maier, Katrin; Ziehm, Patrice; Malessa, Christina; Settmacher, Utz

    2016-01-01

    For a long time, it was considered medical malpractice to neglect the blood group system during transplantation. Because there are far more patients waiting for organs than organs available, a variety of attempts have been made to transplant AB0-incompatible (AB0i) grafts. Improvements in AB0i graft survival rates have been achieved with immunosuppression regimens and plasma treatment procedures. Nevertheless, some grafts are rejected early after AB0i living donor liver transplantation (LDLT) due to antibody mediated rejection or later biliary complications that affect the quality of life. Therefore, the AB0i LDLT is an option only for emergency situations, and it requires careful planning. This review compares the treatment possibilities and their effect on the patients’ graft outcome from 2010 to the present. We compared 11 transplant center regimens and their outcomes. The best improvement, next to plasma treatment procedures, has been reached with the prophylactic use of rituximab more than one week before AB0i LDLT. Unfortunately, no standardized treatment protocols are available. Each center treats its patients with its own scheme. Nevertheless, the transplant results are homogeneous. Due to refined treatment strategies, AB0i LDLT is a feasible option today and almost free of severe complications. PMID:27683633

  3. Red cell antigen prevalence predicted by molecular testing in ethnic groups of South Texas blood donors.

    Science.gov (United States)

    Aranda, Lorena I; Smith, Linda A; Jones, Scott; Beddard, Rachel

    2015-01-01

    Alloimmunization to red blood cell antigens is seen in patients receiving chronic blood transfusion. Knowing the prevalence of blood group antigens of the different ethnicities of South Texas donors can provide better management of rare blood inventory for patients in this geographical area. A total of 4369 blood donors were tested and analyzed for various antigens in the following blood group systems: ABO, Rh, Kell, Duffy, Kidd, MNS, Lutheran, Dombrock, Landsteiner-Wiener, Diego, Colton, and Scianna. Donors tested to be group 0 or A were serologically tested for the Rh (C, E, c, e) antigens. Those that tested as presumably R1R1, R2R2, or Ror were then genotyped. Donors constituted three major ethnicities: black (18.3%), Hispanic (36.3%), and Caucasian (41.1%); ethnicities comprised of Asian, American Indian, multiracial, and other accounted for the remaining donors (4.3%). The most likely common Rh phenotype for each ethnicity is as follows: black -Ror (44.4%), Hispanic -R1R1 (59.0%), and Caucasian -R1R1 (38.9%). The prevalence of Kell, Duffy, and Kidd blood group system antigens in black and Caucasian donors is comparable with published reports for the entire U.S. The black South Texas donor population had an 8.8 percent increase in prevalence of the Fy(a+b-) phenotype as compared with these published reports; the Hispanic South Texas donor population had a prevalence of 36.1 percent of the Fy(a+b-) phenotype. Regarding the Diego blood group system, the Hispanic donor population in South Texas had a prevalence of 93.5 percent for the Di(a-b+) phenotype as compared with published reports for the entire U.S. (>99.9%). The Hispanic population had a prevalence of 7.9 percent of donors testing as M-N+S-s+ as compared with 20.2 percent and 15.6 percent for black and Caucasian donors, respectively. This study helped us determine the prevalence of each of the blood group antigens in the South Texas donor population to establish and maintain adequate rare inventory of

  4. Red cell antigen prevalence predicted by molecular testing in ethnic groups of South Texas blood donors.

    Science.gov (United States)

    Aranda, Lorena I; Smith, Linda A; Jones, Scott; Beddard, Rachel

    2015-01-01

    Alloimmunization to red blood cell antigens is seen in patients receiving chronic blood transfusion. Knowing the prevalence of blood group antigens of the different ethnicities of South Texas donors can provide better management of rare blood inventory for patients in this geographical area. A total of 4369 blood donors were tested and analyzed for various antigens in the following blood group systems: ABO, Rh, Kell, Duffy, Kidd, MNS, Lutheran, Dombrock, Landsteiner-Wiener, Diego, Colton, and Scianna. Donors tested to be group 0 or A were serologically tested for the Rh (C, E, c, e) antigens. Those that tested as presumably R1R1, R2R2, or Ror were then genotyped. Donors constituted three major ethnicities: black (18.3%), Hispanic (36.3%), and Caucasian (41.1%); ethnicities comprised of Asian, American Indian, multiracial, and other accounted for the remaining donors (4.3%). The most likely common Rh phenotype for each ethnicity is as follows: black -Ror (44.4%), Hispanic -R1R1 (59.0%), and Caucasian -R1R1 (38.9%). The prevalence of Kell, Duffy, and Kidd blood group system antigens in black and Caucasian donors is comparable with published reports for the entire U.S. The black South Texas donor population had an 8.8 percent increase in prevalence of the Fy(a+b-) phenotype as compared with these published reports; the Hispanic South Texas donor population had a prevalence of 36.1 percent of the Fy(a+b-) phenotype. Regarding the Diego blood group system, the Hispanic donor population in South Texas had a prevalence of 93.5 percent for the Di(a-b+) phenotype as compared with published reports for the entire U.S. (>99.9%). The Hispanic population had a prevalence of 7.9 percent of donors testing as M-N+S-s+ as compared with 20.2 percent and 15.6 percent for black and Caucasian donors, respectively. This study helped us determine the prevalence of each of the blood group antigens in the South Texas donor population to establish and maintain adequate rare inventory of

  5. Rh D blood group conversion using transcription activator-like effector nucleases.

    Science.gov (United States)

    Kim, Young-Hoon; Kim, Hyun O; Baek, Eun J; Kurita, Ryo; Cha, Hyuk-Jin; Nakamura, Yukio; Kim, Hyongbum

    2015-01-01

    Group O D-negative blood cells are universal donors in transfusion medicine and methods for converting other blood groups into this universal donor group have been researched. However, conversion of D-positive cells into D-negative is yet to be achieved, although conversion of group A or B cells into O cells has been reported. The Rh D blood group is determined by the RHD gene, which encodes a 12-transmembrane domain protein. Here we convert Rh D-positive erythroid progenitor cells into D-negative cells using RHD-targeting transcription activator-like effector nucleases (TALENs). After transfection of TALEN-encoding plasmids, RHD-knockout clones are obtained. Erythroid-lineage cells differentiated from these knockout erythroid progenitor cells do not agglutinate in the presence of anti-D reagents and do not express D antigen, as assessed using flow cytometry. Our programmable nuclease-induced blood group conversion opens new avenues for compatible donor cell generation in transfusion medicine. PMID:26078220

  6. Are anemia and blood group types related to Japanese encephalitis and dengue?

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    Shantanu Sharma

    2015-01-01

    Full Text Available Introduction: Dengue and Japanese encephalitis (JE are flaviviruses causing significant morbidity and mortality worldwide. Previous studies had found an association between dengue infection and particular blood group antigen and also the susceptibility to aplastic anemia by dengue. Aims and Objective: The present study was conducted to find any association between dengue and JE immunoglobulin G (IgG antibody with blood group antigen or anemia. Methodology: The study was conducted in 2 slums of Delhi in 2013 in the interepidemic period. 5 mL blood from 239 samples were taken and processed for IgG levels of dengue and JE and rest used to test hemoglobin and blood group. Results and Discussion: No association has been found between blood group types and dengue IgG antibody positivity (P = 0.42 or JE IgG antibody positivity (P = 0.148. Nor did anemia established any association with dengue (P = 0.185 or JE IgG antibody (P = 0.277. IgG status of dengue and JE could be used as proxy markers of any past subclinical infection (includes clinically manifested cases also. Further studies in large settings and other areas could be done to validate the results. This was the first study of its kind in such settings.

  7. Hepatitis G Virus Infection in Iranian Blood Donors and High-Risk Groups

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    Shahram Samiei

    2009-11-01

    Full Text Available Background and Aims: Hepatitis G virus (HGV has a worldwide distribution, and the prevalence rates among blood donors and high-risk groups are different. The purpose of this study was to assess the frequency of the HGV infection in blood donors as a blood borne pathogen and in high-risk groups (multitransfused patients, such as thalassemic, hemophillic, and hemodialysis patients.Methods: 400 Iranian (Tehran Blood Transfusion Center, 2004 blood donors were tested for HGV RNA by a reverse transcriptase chain reaction (RT-PCR method. The participants were negative in blood screening tests for hepatitis B surface antigen (HBsAg, hepatitis C virus antibodies (anti- HCV, human immunodeficiency virus (HIV Ag/Ab, and Rapid Plasma Reagin (RPR. HGV RNA positivity was surveyed in 40 thalassaemic, 16 hemophilic, and 46 hemodialysis patients by RT-PCR. To assess the frequency of infection, the prevalence of HGV RNA positive cases per 100 donors/patients with 95% confidence intervals (95% CI were calculated. P values were estimated with chi-square tests.Results: 19 (4.8%; 95% CI: 2.9-6.5% out of 400 blood donors samples were HGV RNA positive. The prevalence of HGV infection was 5.28% (13 out of 243 in repeat donors, 4.12% (4 in 99 in lapsed donors, and 3.50% (2 out of 58 in first-time blood donors. The combined prevalence of HGV infection in these groups of patients was 16 (15.7%; 95% CI: 8.3-23.1% out of 102 samples. HGV RNA frequency was 1 out of 40 (2.5%; 95% CI: 1.8-3.2% thalassemic patients, 15 out of 46 (32.6%; 95% CI: 16.8-48.4% hemodialysis patients, and 0 out of 16 hemophilics patients.Conclusions: The prevalence of HGV RNA in the high-risk population was 15.7% and nearly 3 times more than blood donors (4.8%. These data indicate the possibility of parenteral transmission of HGV, especially by transfusion of blood and blood components. Decisions to screen blood supplies for a transfusion-transmitted infection agent should be based on sufficient

  8. ABO Blood Group Genotyping by Real-time PCR in Kazakh Population

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    Pavel Tarlykov

    2014-12-01

    Full Text Available Introduction. ABO blood group genotyping is a new technology in hematology that helps prevent adverse transfusion reactions in patients. Identification of antigens on the surface of red blood cells is based on serology; however, genotyping employs a different strategy and is aimed directly at genes that determine the surface proteins. ABO blood group genotyping by real-time PCR has several crucial advantages over other PCR-based techniques, such as high rapidity and reliability of analysis. The purpose of this study was to examine nucleotide substitutions differences by blood types using a PCR-based method on Kazakh blood donors.Methods. The study was approved by the Ethics Committee of the National Center for Biotechnology. Venous blood samples from 369 healthy Kazakh blood donors, whose blood types had been determined by serological methods, were collected after obtaining informed consent. The phenotypes of the samples included blood group A (n = 99, B (n = 93, O (n = 132, and AB (n = 45. Genomic DNA was extracted using a salting-out method. PCR products of ABO gene were sequenced on an ABI 3730xl DNA analyzer (Applied Biosystems. The resulting nucleotide sequences were compared and aligned against reference sequence NM_020469.2. Real-time PCR analysis was performed on CFX96 Touch™ Real-Time PCR Detection System (BioRad.Results. Direct sequencing of ABO gene in 369 samples revealed that the vast majority of nucleotide substitutions that change the ABO phenotype were limited to exons 6 and 7 of the ABO gene at positions 261, 467, 657, 796, 803, 930 and 1,060. However, genotyping of only three of them (261, 796 and 803 resulted in identification of major ABO genotypes in the Kazakh population. As a result, TaqMan probe based real-time PCR assay for the specific detection of genotypes 261, 796 and 803 was developed. The assay did not take into account several other mutations that may affect the determination of blood group, because they have a

  9. Molecular basis for H blood group deficiency in Bombay (Oh) and para-Bombay individuals.

    Science.gov (United States)

    Kelly, R J; Ernst, L K; Larsen, R D; Bryant, J G; Robinson, J S; Lowe, J B

    1994-06-21

    The penultimate step in the biosynthesis of the human ABO blood group oligosaccharide antigens is catalyzed by alpha-(1,2)-fucosyltransferase(s) (GDP-L-fucose: beta-D-galactoside 2-alpha-L-fucosyltransferase, EC 2.4.1.69), whose expression is determined by the H and Secretor (SE) blood group loci (also known as FUT1 and FUT2, respectively). These enzymes construct Fuc alpha 1-->2Gal beta-linkages, known as H determinants, which are essential precursors to the A and B antigens. Erythrocytes from individuals with the rare Bombay and para-Bombay blood group phenotypes are deficient in H determinants, and thus A and B determinants, as a consequence of apparent homozygosity for null alleles at the H locus. We report a molecular analysis of a human alpha-(1,2)-fucosyltransferase gene, thought to correspond to the H blood group locus, in a Bombay pedigree and a para-Bombay pedigree. We find inactivating point mutations in the coding regions of both alleles of this gene in each H-deficient individual. These results define the molecular basis for H blood group antigen deficiency in Bombay and para-Bombay phenotypes, provide compelling evidence that this gene represents the human H blood group locus, and strongly support a hypothesis that the H and SE loci represent distinct alpha-(1,2)-fucosyltransferase genes. Candidate sequences for the human SE locus are identified by low-stringency Southern blot hybridization analyses, using a probe derived from the H alpha-(1,2)-fucosyltransferase gene.

  10. Risk Factors, Coronary Severity, Outcome and ABO Blood Group: A Large Chinese Han Cohort Study.

    Science.gov (United States)

    Zhang, Yan; Li, Sha; Zhu, Cheng-Gang; Guo, Yuan-Lin; Wu, Na-Qiong; Xu, Rui-Xia; Dong, Qian; Liu, Geng; Li, Jian-Jun

    2015-10-01

    ABO blood type locus has been reported to have ethnic difference and to be a pivotal genetic determinant of cardiovascular risk, whereas few prospective data regarding the impact on cardiovascular outcomes are available in a large cohort of patients with angiography-proven coronary artery disease, especially from the Chinese population. The objective of this study was to assess the prognostic role of blood type in future cardiovascular events (CVEs) in Chinese Han patients undergoing coronary angiography.The population of this prospective cohort study consisted of 3823 eligible patients, and followed annually to capture all CVEs. Baseline characteristics and ABO blood type were obtained. Cox proportional hazards models were used to evaluate the risk of ABO blood type on CVEs.New CVEs occurred in 348 patients [263 (10.3%) non-O and 85 (7.8%) O] during a median period of 24.6 months follow-up. Significantly, non-O blood group was related to the presence and severity of coronary atherosclerosis and several risk factors including inflammatory markers. The log-rank test revealed that there was a significant difference between non-O and O blood groups in event-free survival analysis (P = 0.026). In particular, the Cox proportional hazards models revealed that non-O blood type was associated with increased CVEs risk [hazard ratio (95% confidence interval) 1.320 (1.033-1.685)], even after adjusting for potential confounders [adjusted hazard ratio (95% confidence interval) non-O: 1.289 (1.003-1.656); A: 1.083 (0.797-1.472); B: 1.481 (1.122-1.955); AB: 1.249 (0.852-1.831), respectively].Non-O blood type is associated with future CVEs in Chinese Han patients undergoing coronary angiography.

  11. Immobilization to prevent enzyme incompatibility with proteases

    NARCIS (Netherlands)

    Vossenberg, P.; Beeftink, H.H.; Cohen Stuart, M.A.; Tramper, J.

    2011-01-01

    Enzyme incompatibility is a problem in multi-enzyme processes that involve a non-specific protease, such as Alcalase. An example is the one-pot enzymatic synthesis of peptides catalyzed by a lipase and a protease. The incompatibility between lipase B from Candida antarctica (CalB) and Alcalase was s

  12. Introduction of phosphoric acid group to polypropylene film by radiation grafting and its blood compatibility

    International Nuclear Information System (INIS)

    2,3-epoxypropyl methacrylate (EPMA) was grafted to polypropylene (PP) film by using a radiation grafting technique. The phosphoric acid group was introduced to the EPMA-grafted PP films with different grafting yields. The blood compatibility of the phosphoric acid group-introduced PP films was evaluated by the determination of platelet adsorption and thrombus formation. The EPMA grafting extent was found to be dependent on the absorbed dose, reaction time and temperature. The grafting and phosphonation reactions were confirmed by Fourier transform infrared spectroscopy in the attenuated total reflectance mode and electron spectroscopy for chemical analysis. The amount of thrombus and adherent platelet on modified PP film was evaluated by an in vitro method and scanning electron microscope, respectively. The phosphoric acid group-introduced PP film was found to have good blood compatibility, which increased with the content of the introduced phosphoric acid group

  13. Genogroup IV and VI Canine Noroviruses Interact with Histo-Blood Group Antigens

    OpenAIRE

    Caddy, Sarah ,; Breiman, Adrien; Le Pendu, Jacques; Goodfellow, Ian

    2014-01-01

    ABSTRACT Human noroviruses (HuNV) are a significant cause of viral gastroenteritis in humans worldwide. HuNV attaches to cell surface carbohydrate structures known as histo-blood group antigens (HBGAs) prior to internalization, and HBGA polymorphism among human populations is closely linked to susceptibility to HuNV. Noroviruses are divided into 6 genogroups, with human strains grouped into genogroups I (GI), II, and IV. Canine norovirus (CNV) is a recently discovered pathogen in dogs, with s...

  14. Bombay blood group: Is prevalence decreasing with urbanization and the decreasing rate of consanguineous marriage

    Directory of Open Access Journals (Sweden)

    Sujata Mallick

    2015-01-01

    Full Text Available Context: Bombay blood group although rare is found to be more prevalent in the Western and Southern states of India, believed to be associated with consanguineous marriage. Aims: To estimate the prevalence of the Bombay blood group (Oh in the urban population of Puducherry. To find the effect of urbanization on consanguineous marriage and to establish whether consanguinity plays a part in the prevalence of Oh group. To compare Oh group prevalence with that of other neighboring states, where population is not predominantly urban. Settings and Design: This is a descriptive study in a tertiary care hospital in Puducherry, over a period of 6 years. Materials and Methods: All blood samples showing ′O′ group were tested with anti-H lectin. Specialized tests like Adsorption Elution Technique, inhibition assay for determination of secretor status were performed on Oh positive cases. Any history of consanguineous marriage was recorded. Statistical Analysis Used: All variables were categorical variable and percentage and proportions were calculated manually. Results: Analysis of the results of 35,497 study subjects showed that the most common group was ′O′ group constituting 14,164 (39.90% of subjects. Only three "Oh" that is, Bombay phenotype (0.008% were detected. Consanguinity was observed in two cases (66.66%. Conclusions: This study shows the prevalence of Bombay blood group representing the urban population of Puducherry, to be high (0.008% and associated with consanguineous marriage (66.66%. Thus, consanguinity is still an important risk factor present, even in an urban population in Southern India.

  15. Association of ABO Blood Group Phenotype and Allele Frequency with Chikungunya Fever

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    Pairaya Rujirojindakul

    2015-01-01

    Full Text Available Background. The objective of this study was to investigate the association of the ABO blood group phenotype and allele frequency with CHIK fever. Methods. A rural community survey in Southern Thailand was conducted in August and September 2010. A total of 506 villagers were enrolled. Cases were defined as individuals having anti-CHIK IgG by hemagglutination ≥1 : 10. Results. There were 314 cases (62.1% with CHIK seropositivity. Females were less likely to have positive anti-CHIK IgG with odds ratio (OR (95% CI of 0.63 (0.43, 0.93. All samples tested were Rh positive. Distribution of CHIK seropositivity versus seronegativity (P value in A, B, AB, and O blood groups was 80 versus 46 (0.003, 80 versus 48 (0.005, 24 versus 20 (0.55, and 130 versus 78 (<0.001, respectively. However, chi-square test between ABO and CHIK infection showed no statistical significance P=0.76. Comparison of the ABO blood group allele frequency between CHIK seropositivity and seronegativity was not statistically significant. Conclusion. This finding demonstrated no association of the ABO blood group phenotypes and allele frequencies with CHIK infection.

  16. Association of ABO blood groups with glaucoma in the Pakistani population.

    NARCIS (Netherlands)

    Khan, M.I.; Micheal, S.; Akhtar, F.; Naveed, A.; Ahmed, A.; Qamar, R.

    2009-01-01

    OBJECTIVE: To study the association of blood groups with different types of glaucoma including primary open-angle glaucoma (POAG), primary closed-angle glaucoma (PCAG), and pseudoexfoliative glaucoma (PEXG) in the Pakistani population. STUDY DESIGN: The present study was a prospective case control s

  17. Genetic and epigenetic alterations of the blood group ABO gene in oral squamous cell carcinoma

    DEFF Research Database (Denmark)

    Gao, Shan; Worm, Jesper; Guldberg, Per;

    2004-01-01

    Loss of histo-blood group A and B antigen expression is a frequent event in oral carcinomas and is associated with decreased activity of glycosyltransferases encoded by the ABO gene. We examined 30 oral squamous cell carcinomas for expression of A and B antigens and glycosyltransferases. We also...

  18. Histo-blood group antigens in human fetal thymus and in thymomas

    DEFF Research Database (Denmark)

    Engel, P; Dabelsteen, Erik; Francis, D;

    1996-01-01

    -y, Le-x and sialyl-Le-x) of the ABO-histo-blood group system was investigated in 19 normal fetal thymuses (gestational age 16 to 39 weeks) and in 19 thymomas in order to study possible tumor-associated changes in the glycosylation pattern. The material was investigated by immunochemical stainings...

  19. DETECTION OF A RARE BLOOD GROUP “BOMBAY (OH PHENOTYPE” IN A POST CAESAREAN PREGNANCY WITH ANAEMIA - A RARE CASE REPORT FROM EASTERN INDIA

    Directory of Open Access Journals (Sweden)

    Anindya Kumar

    2013-10-01

    Full Text Available ABSTRACT: The Bombay blood group is a very rare blood group discovered almost 60 years back. We report here, a high risk case of Post Caesarean pregnancy with anaemia with Bombay Blood Group

  20. Genotyping for Kidd, Kell, Duffy, Scianna, and RHCE blood group antigens polymorphisms in Jiangsu Chinese Han

    Institute of Scientific and Technical Information of China (English)

    LIU Zhong; ZHANG Xue-guang; ZENG Rong; CHEN Qing; LI Min; SHI Guang-yao; WEI Peng; HUANG Cheng-yin; TANG Rong-cai; SUN Jun

    2012-01-01

    Background Molecular testing is more precise compared to serology and has been widely used in genotyping blood group antigens.Single nucleotide polymorphisms (SNPs) of blood group antigens can be determined by the polymerase chain reaction with sequence specific priming (PCR-SSP) assay.Commercial high-throughput platforms can be expensive and are not approved in China.The genotype frequencies of Kidd,Kell,Duffy,Scianna,and RhCE blood group antigens in Jiangsu province were unknown.The aim of this study is sought to detect the genotype frequencies of Kidd,Kell,Duffy,Scianna,and RhCE antigens in Jiangsu Chinese Han using molecular methods with laboratory developed tests.Methods DNA was extracted from EDTA-anticoagulated blood samples of 146 voluntary blood donors collected randomly within one month.Standard serologic assay for red blood cell antigens were also performed except the Scianna blood group antigens.PCR-SSP was designed to work under one PCR program to identify the following SNPs:JK1/JK2,KEL 1/KEL2,FYA/FYB,SC1/SC2,C/c and E/e.Results Serologic antigen results were identical to the phenotypes that were predicted from genotyping results.The allele frequencies for Jk*01 and Jk*02 were 0.51 and 0.49,respectively; for Fy*A and Fy*B 0.94 and 0.06; for RHCE*C and RHCE*c 0.68 and 0.32; and for RHCE*E and RHCE*e 0.28 and 0.72.Among 146 blood donors,all were KEL*02/KEL*02 and SC*01/SC*01,indicating allele frequencies for KEL*02 and SC*01 close to 1.00.Conclusions The use of PCR-SSP working under the same condition for testing multiple antigens at the same time is practical.This approach can be effective and cost-efficient for small-scale laboratories and in developing counties.These molecular tests can be also used for identifying rare blood types.

  1. Blood selenium levels and contribution of food groups to selenium intake in adolescent girls in Iceland

    Directory of Open Access Journals (Sweden)

    Ingibjorg Gunnarsdottir

    2012-08-01

    Full Text Available Background/objectives: Significant changes have been reported in dietary habits and food availability in Iceland that would be expected to compromise selenium intake and status, especially among young people. These include substantial decreases in the consumption of fish and milk, as well as the selenium content of imported wheat. The aim of this study was to assess selenium in the diet and whole blood of adolescent girls, as well as define the most important foods contributing to intake and blood concentrations of selenium. Design: The subjects were 96 randomly selected girls, aged 16–20, who answered a validated food frequency questionnaire (FFQ for dietary assessment. Selenium intake from each food group was calculated in µg/day. Blood samples were collected for measurement of whole blood selenium. Results: Mean dietary selenium was 51±25 µg/day. Milk/dairy products, including cheese, contributed 36±14% of total dietary selenium; fish 18±12%; and bread/cereal products 13±6%. Mean whole blood selenium was 117±12 µg/l (range 90–208; nearly 90% of subjects were above the optimal level of 100 µg/l. Fish and bread/cereal products were the only foods significantly correlated with selenium in blood (r=0.32; P = 0.002 and r=0.22; P = 0.04, respectively while no correlation was found with milk and dairy products in spite of their greater contribution to total selenium intake. Conclusion: In this population of Icelandic adolescent girls, selenium intake and status seem acceptable. Judging from associations between intake and blood levels, fish and cereals may be the most important contributors to blood selenium.

  2. Duffy blood group genotypes among malariaPlasmodiumvivax patients of Baoulch population in southeastern Iran

    Institute of Scientific and Technical Information of China (English)

    Ebrahim Miri-Moghaddam; Zakaria Bameri; Mehdi Mohamadi

    2014-01-01

    Objective:To determine the distribution ofDuffy blood group genotypes inBalouch population as a major ethnic group that living in a sub-tropical area in southEast ofIran.Methods:In this study, theDuffy blood groupFY phenotypes were determined using indirect anti-globulin technique and also genotype byPCR-RFLP in160 vivax malaria patients and160 control individuals.Results:The results showed that the most commonDuffy genotype wasFYA/FYB (46.6%) followed byFYA/FYA(15.3%),FYA/FYO(14.4%),FYB/FYO(11.9%),FYB/FYB(10%) and FYO/FYO(1.9%).In case individuals, frequency ofFYA,FYB andFYO alleles were0.471,0.431 and0.097, respectively compaired to0.444,0.353 and0.203, respectively in control(non-infected) group.Conclusions:This data provide evidence that individuals with theFYA/FYB genotype have higher susceptibility to malaria and there are significant associations betweenDuffy blood group variants and susceptibility or resistance to vivax malaria.

  3. Linkage disequilibrium between the ELA and the A blood group systems in Standardbred horses.

    Science.gov (United States)

    Bailey, E

    1983-01-01

    The linkage group formed by the ELA and A blood group system in horses was studied in American Standardbred horses. The distance between the ELA locus and the A blood group locus was measured as 1.61 centimorgans, observing only the haplotypes contributed by the sires. Strong linkage disequilibrium was found in pacing Standardbred horses for ELA-W1 with Aa, ELA-W5 with Ab and ELA-W10 with Ab. Linkage disequilibrium was apparent at both the population and family level. Among trotting Standardbred horses, linkage disequilibrium was found for ELA-W1 with Aa and for ELA-W10 with Ab. It was not possible to investigate linkage relationships in Thoroughbred horses because of the high frequency of Aa and low frequency of other A system markers.

  4. Abo and Rh Blood Groups Distribution in Hemophilia and Anti Hiv Positive Individuals

    Directory of Open Access Journals (Sweden)

    D.D. FARHUD

    1987-07-01

    Full Text Available A group of Iranian patients suffering from Factor VIII deficiency (Hemophilia A and treated with contaminated coagulation factor (imported, became seropositive as determined by ELISA method. Sixty of these individuals, which were available, were studied for ABO distribution. The B blood group in anti HIV pos. individuals (13.33% shows a significant decrease in comparison with the total (1504 of factor VIII hemophilia (21.87%. Statistical analysis of ABO distribution in anti HIV Pos. compared with hemophilia A and the control group showed x2 values of 6.86(0.10 > p>0.05 and 10.21(0.02> P >0.01 respectively.

  5. Phenotype frequencies of blood group systems (Rh, Kell, Kidd, Duffy, MNS, P, Lewis, and Lutheran) in blood donors of south Gujarat, India

    OpenAIRE

    Manoj A Kahar; Patel, Rajnikant. D.

    2014-01-01

    Background: This is the first study on phenotype frequencies of various blood group systems in blood donors of south Gujarat, India using conventional tube technique. Material and Methods: A total of 115 "O" blood group donors from three different blood banks of south Gujarat were typed for D, C, c, E, e, K, Jk a , Le a , Le b , P 1 , M, and N antigens using monoclonal antisera and k, Kp a , Kp b , Fy a ,Fy b , Jk b , S,s, Lu a , and Lu b antigens were typed using polyclonal antisera employin...

  6. Relation between ABO blood groups and Helicobacter pylori infection in symptomatic patients

    Directory of Open Access Journals (Sweden)

    Jaff MS

    2011-09-01

    Full Text Available Mohamad Salih Jaff Pathology Department, College of Medicine, Hawler Medical University (formerly Salahuddin University, Erbil, Kurdistan Region, Iraq Abstract: Epidemiological studies have demonstrated higher frequencies of the O blood group and the nonsecretor phenotype of ABH antigens among patients suffering from peptic ulcers. Since Helicobacter pylori has been established as the main etiological factor in this disease, controversies about the associations of the ABO and Lewis blood group phenotypes and secretor and nonsecretor phenotypes in relation to susceptibility towards infection by this bacillus have been presented. The aim of this study was to verify the frequencies of ABO and Rhesus (Rh blood groups in H. pylori seropositive symptomatic patients. The study included (n = 1108 patients with dyspepsia symptoms referred from an outpatient clinic in Erbil city for investigation. Age, sex, and residency were recorded as a routine laboratory framework. Patients underwent SD Bioline (Standard Diagnostics Inc, Kyonggi-do, South Korea and enzyme-linked immunosorbent assay serologic tests for H. pylori. ABO blood group phenotypes were determined by a standard hemagglutination test. Results showed that 64.8% of patients (n = 718/1108 were seropositive for H. pylori infection, and (35.2% (n = 390/1108 were seronegative. Of the seropositive patients, 40.8% (n = 293/718 were male and 59.2% (n = 425/718 were female; while of the seronegative patients, 46.7% (n = 182/390 were male and 53.3% (n = 208/390 were female. The mean age for seropositives and seronegatives was (38.0 ± 14.6 years and (37.6 ± 15.7 years respectively. The frequency of the ABO and Rh-positive (Rh+ blood groups among seropositive patients was (A = 32.0%, B = 19.5%, AB = 6.7%, O = 41.8%, and Rh+ = 92.5% and was (A = 32.3%, B = 28.2%, AB = 8.0%, O = 31.5%, and Rh+ = 92.5% in seronegatives. The results of this study suggest that ABO blood groups, age, and gender influence

  7. Anthropometric, environmental, and dietary predictors of elevated blood cadmium levels in Ukrainian children: Ukraine ELSPAC group

    International Nuclear Information System (INIS)

    No comprehensive data on sources or risk factors of cadmium exposure in Ukrainian children are available. In this we measured the blood levels of cadmium among 80 Ukrainian children and evaluated sources of exposure. A nested case-control study from a prospective cohort of Ukrainian 3-year-old children was conducted. We evaluated predictors of elevated blood cadmium using a multivariable logistic regression model. The model included socioeconomic data, parent occupation, environmental tobacco smoke, hygiene, body-mass index, and diet. Dietary habits were evaluated using the 1992 Block-NCI-HHHQ Dietary Food Frequency survey. Elevated cadmium was defined as blood levels in the upper quartile (>=0.25μg/L). The mean age for all 80 children was 36.6 months. Geometric mean cadmium level was 0.21μg/L (range=0.11-0.42μg/L; SD=0.05). Blood cadmium levels were higher among children taking zinc supplements (0.25 vs 0.21μg/L; P=0.032), children who ate sausage more than once per week (0.23 vs 0.20; P=0.007) and children whose fathers worked in a by-product coking industry (0.25 vs 0.21; P=0.056). In the multivariable model, predictors of elevated blood cadmium levels included zinc supplementation (adjusted OR=14.16; P<0.01), father working in a by-product coking industry (adjusted OR=8.50; P=0.03), and low body mass index (<14.5; adjusted OR=5.67; P=0.03). This is the first study to indicate a strong association between elevated blood cadmium levels and zinc supplementation in young children. Whole-blood cadmium levels observed in this group of Ukrainian children appear to be similar to those reported in other Eastern European countries

  8. Aplasia pura de serie roja post-trasplante alogeneico de células progenitoras hematopoyeticas ABO incompatible Pure red cell aplasia after ABO incompatible bone marrow transplantation

    Directory of Open Access Journals (Sweden)

    E. Bulliorsky

    2002-12-01

    titers against donor's blood group. PRCA did not improve after treatment with erythropoietin or a boost of hematopoietic progenitor cells obtained from donor's peripheral blood but the problem was resolved completely after treatment with ALG.

  9. Maternal ABO and rhesus blood group phenotypes and hepatitis B surface antigen carriage.

    Science.gov (United States)

    Lao, T T; Sahota, D S; Chung, M-K; Cheung, T K W; Cheng, Y K Y; Leung, T Y

    2014-11-01

    In view of a persistently high prevalence of hepatitis B surface antigen (HBsAg) carriage in our obstetric population, we examined the association between HBsAg carriage with maternal ABO and rhesus (Rh) blood group phenotypes determined at routine antenatal screening. In a retrospective study, the antenatal screening results of women booked for confinement between 1998 and 2011 in our hospital were examined for the relationship between HBsAg carriage with the ABO and rhesus blood groups, taking into account also the effects of advanced maternal age (≥ 35 years) and parity status (nulliparous or multiparous), and year of birth before or following the availability of the hepatitis B vaccine (1984). HBsAg carriage was found in 9.9%, 9.6%, 9.1% and 10.2% (P = 0.037) for group-A (n = 20 581 or 26.1%), -B (n = 20 744 or 26.4%), -AB (n = 5138 or 6.5%) and -O (n = 32 242 or 41.0%) among the 78705 women in the study cohort. Rhesus negativity was found in 0.6%, and HBsAg carriage was 12.3% and 9.8%, respectively, for the Rh-negative and Rh-positive women (P = 0.071). Carriage rate between group-O and non-O was influenced by nulliparity, age ≥ 35 years and Rh-positive status. Regression analysis indicated that group-B (P = 0.044, aOR = 1.062, 95% CI 1.002-1.127) and group-AB (P = 0.016, aOR = 1.134, 95% CI 1.024-1.256) were associated with HBsAg carriage. Blood groups-B and -AB are associated with increased hepatitis B virus (HBV) infection in our population, and further studies are warranted to elucidate the implications of this on the sequelae of HBV infection.

  10. Erythrocyte-bound apolipoprotein B in relation to atherosclerosis, serum lipids and ABO blood group.

    Directory of Open Access Journals (Sweden)

    Boudewijn Klop

    Full Text Available INTRODUCTION: Erythrocytes carry apolipoprotein B on their membrane, but the determining factors of erythrocyte-bound apolipoprotein B (ery-apoB are unknown. We aimed to explore the determinants of ery-apoB to gain more insight into potential mechanisms. METHODS: Subjects with and without CVD were included (N = 398. Ery-apoB was measured on fresh whole blood samples using flow cytometry. Subjects with ery-apoB levels ≤ 0.20 a.u. were considered deficient. Carotid intima media thickness (CIMT was determined as a measure of (subclinical atherosclerosis. RESULTS: Mean ery-apoB value was 23.2% lower in subjects with increased CIMT (0.80 ± 0.09 mm, N = 140 compared to subjects with a normal CIMT (0.57 ± 0.08 mm, N = 258 (P = 0.007, adjusted P<0.001. CIMT and ery-apoB were inversely correlated (Spearman's r: -0.116, P = 0.021. A total of 55 subjects (13.6% were considered ery-apoB deficient, which was associated with a medical history of CVD (OR: 1.86, 95% CI 1.04-3.33; adjusted OR: 1.55; 95% CI 0.85-2.82. Discontinuation of statins in 54 subjects did not influence ery-apoB values despite a 58.4% increase in serum apolipoprotein B. Subjects with blood group O had significantly higher ery-apoB values (1.56 ± 0.94 a.u. when compared to subjects with blood group A (0.89 ± 1.15 a.u, blood group B (0.73 ± 0.1.12 a.u. or blood group AB (0.69 ± 0.69 a.u. (P-ANOVA = 0.002. CONCLUSION: Absence or very low values of ery-apoB are associated with clinical and subclinical atherosclerosis. While serum apolipoprotein B is not associated with ery-apoB, the ABO blood group seems to be a significant determinant.

  11. Blood group and serum protein polymorphisms in turpu kapu population of vizianagaram district, Andhra Pradesh

    Directory of Open Access Journals (Sweden)

    V Komal Madhavi

    2002-01-01

    Full Text Available Data on two blood group and three serum protein polymorphisms of the Turpu Kapu, an endogamous population of Vizianagaram District, Andhra Pradesh (AP is presented. The gene frequencies for the blood group systems ABO and Rh are within the ranges of distribution reported earlier among the caste populations of Andhra Pradesh. The study population shows highest frequency of Hp1 allele and the lowest frequency of Hp2 allele compared to the other populations of AP. The Cp system is monomorphic, all individuals being the BB type. The GC system exhibits polymorphism with the gene frequencies of GC1 and GC2 alleles showing the highest and lowest frequencies, respectively, as compared to the caste populations reported earlier. The c2 test suggest that this population is in Hardy-Weinberg Equilibrium.

  12. Reduced frequency of blood group Lewis a-b- in female Type 1 diabetes patients

    DEFF Research Database (Denmark)

    Kharagjitsingh, A.V.; Prinsen, K.; Lemkes, H.H.;

    2008-01-01

    population. RESULTS: T1D patients had a lower frequency (4.1%) of Lewis(a(-)b(-)) blood group compared with simultaneously tested healthy control subjects (10.0%) and the historical control group (11.1%, P = 0.02). Male T1D patients showed a Lewis(a(-)b(-)) frequency of 8.0%, which was similar to both......AIMS: To examine a disputed association between the Lewis(a(-)b(-)) phenotype and Type 1 diabetes (T1D). METHODS: Lewis red blood cell phenotyping was performed for 97 T1D White patients and 100 control subjects using monoclonal antibodies. Two historical cohorts were also included as a control...

  13. Lichenoid reaction associated with silver amalgam restoration in a Bombay blood group patient: A case report

    OpenAIRE

    Rohini Rangarao Pawar; Mattigatti, Sudha S.; Rushikesh R Mahaparale; Amit P Kamble

    2016-01-01

    The pathogenic relationship between the oral lichenoid reaction (OLR) and dental restorative materials has been confirmed many times. An OLR affecting oral mucosa in direct contact with an amalgam restoration represents a delayed, type IV, cell mediated immune response to mercury or one of the other constituents of the dental amalgam. Bombay blood group patients are more prone to this. A case of bilateral OLR is presented, which is present in relation to amalgam restoration. The lesion healed...

  14. 跨血型肝移植26例经验%Experiences of 26 cases in liver transplantation across ABO blood group barrier

    Institute of Scientific and Technical Information of China (English)

    金磊; 殷浩; 傅宏; 倪之嘉; 马钧; 郭闻渊; 施晓敏; 韩秋成; 傅志仁

    2014-01-01

    Objective To summarize the experiences of 26 case of liver transplantation across ABO barrier in our hospital,and on the basis of literature review,further discuss the feasibility of ABO-incompatible liver transplantation.Methods The clinical data of 395 caces of liver transplantation since January 2009 to December 2012 were retrospectively analyzed.Of them,26 cases were across ABO blood group,including 17 ABO-compatible (ABO-C) and 9 ABO-incompatible (ABO-I).The patients were grouped as ABO-identical(ABO-Id,n =369),ABO-compatible(ABO-C,n =17) and ABO-incompatible(ABO-I,n =9),patient survivals and the rates of biliary complications,vascular complications,infection,acute and chronic rejection were compared among 3 groups.Results the 1-year and 2-year patient's survival rates were 87.3%,82.4%,88.9%,and 78.3%,70.6%,66.7% in ABO-Id,ABO-C,ABO-I group,respectively.The accumulative survival rates among 3 groups were not significantly different (log rank test,P > 0.05).The incidence of general complications and biliary complications was higher in ABO-I than in ABO-Id(P =0.035,P =0.001).There were no significant differences among the 3 groups in vascular complications,infection,acute and chronic rejection (P > 0.05).Conclusions Although the complication incidence is higher in ABO-incompatible liver transplantation,the patient survival rate is compatible to ABO-compatible liver transplantation.Thus,for patients who are waiting grafts for a long time or need emergent liver transplant,ABO-incompatible liver transplantation is safe and feasible.%目的 总结本中心26例跨血型肝移植经验,在复习文献的基础上,探讨血型不相容肝移植的可行性.方法 回顾性分析本中心2009年1月至2011年12月395例肝移植患者病历资料,其中跨血型肝移植26例(血型相容17例,血型不相容9例).根据供受体血型匹配情况分为3组:血型相符(ABO-Id)组369例、血型相容(ABO-C)组17例和血型不相容(ABO-I)组9例.分

  15. No Distinction of Orthology/Paralogy between Human and Chimpanzee Rh Blood Group Genes.

    Science.gov (United States)

    Kitano, Takashi; Kim, Choong-Gon; Blancher, Antoine; Saitou, Naruya

    2016-03-01

    On human (Homo sapiens) chromosome 1, there is a tandem duplication encompassing Rh blood group genes (Hosa_RHD and Hosa_RHCE). This duplication occurred in the common ancestor of humans, chimpanzees (Pan troglodytes), and gorillas, after splitting from their common ancestor with orangutans. Although several studies have been conducted on ape Rh blood group genes, the clear genome structures of the gene clusters remain unknown. Here, we determined the genome structure of the gene cluster of chimpanzee Rh genes by sequencing five BAC (Bacterial Artificial Chromosome) clones derived from chimpanzees. We characterized three complete loci (Patr_RHα, Patr_RHβ, and Patr_RHγ). In the Patr_RHβ locus, a short version of the gene, which lacked the middle part containing exons 4-8, was observed. The Patr_RHα and Patr_RHβ genes were located on the locations corresponding to Hosa_RHD and Hosa_RHCE, respectively, and Patr_RHγ was in the immediate vicinity of Patr_RHβ. Sequence comparisons revealed high sequence similarity between Patr_RHβ and Hosa_RHCE, while the chimpanzee Rh gene closest to Hosa_RHD was not Patr_RHα but rather Patr_RHγ. The results suggest that rearrangements and gene conversions frequently occurred between these genes and that the classic orthology/paralogy dichotomy no longer holds between human and chimpanzee Rh blood group genes. PMID:26872772

  16. ABO/Rh Blood Groups and Risk of HIV Infection and Hepatitis B Among Blood Donors of Abidjan, Côte D'ivoire.

    Science.gov (United States)

    Siransy, Liliane Kouabla; Nanga, Zizendorf Yves; Zaba, Flore Sandrine; Tufa, Nyasenu Yawo; Dasse, Sery Romuald

    2015-09-01

    Hepatitis B and HIV infection are two viral infections that represent real global public health problems. In order to improve their management, some hypotheses suggest that genetic predispositions like ABO and Rh blood groups would influence the occurrence of these diseases. The aim of the present study was to examine the association between ABO and Rhesus blood groups and the susceptibility to HIV infection and hepatitis B. We conducted a cross-sectional and analytical study in a population of voluntary blood donors in the Blood Transfusion Center of Abidjan. All blood donors who donated blood between January and June 2014 were tested for HBs antigen and anti-HIV antibodies (ELISA tests) and were ABO typed. The total number of examined blood donors during this period was 45,538, of which 0.32% and 8.07% were respectively infected with HIV and hepatitis B virus. O-group donors were more infected than non-O donors. Our study is an outline concerning the search for a link between ABO and Rh blood groups and hepatitis B and HIV infection. Further studies should be conducted to confirm the interaction between these two infections and contribute to the search for new therapeutic approaches.

  17. American Society of Blood and Marrow Transplantation, European Society of Blood and Marrow Transplantation, Blood and Marrow Transplant Clinical Trials Network, and International Myeloma Working Group Consensus Conference on Salvage Hematopoietic Cell Transplantation in Patients with Relapsed

    DEFF Research Database (Denmark)

    Giralt, Sergio; Garderet, Laurent; Durie, Brian;

    2015-01-01

    not been extensively studied in MM patients relapsing after primary therapy. The International Myeloma Working Group together with the Blood and Marrow Transplant Clinical Trials Network, the American Society of Blood and Marrow Transplantation, and the European Society of Blood and Marrow Transplantation...

  18. No evidence for a direct effect of von Willebrand factor's ABH blood group antigens on von Willebrand factor clearance

    NARCIS (Netherlands)

    Groeneveld, D J; van Bekkum, T; Cheung, K L; Dirven, R J; Castaman, G; Reitsma, P H; van Vlijmen, B; Eikenboom, J

    2015-01-01

    BACKGROUND: One of the major determinants of von Willebrand factor (VWF) plasma levels is ABO blood group status, and individuals with blood group O have ~ 25% lower plasma levels. The exact mechanism behind this relationship remains unknown, although effects on clearance have been postulated. OBJEC

  19. A case of passenger lymphocyte syndrome following minor ABO incompatible renal transplantation

    Directory of Open Access Journals (Sweden)

    Anju Dubey

    2014-01-01

    Full Text Available Immune hemolysis is one of the adverse effects that can occur following solid organ transplantation. Understanding the clinical settings and the various causes is necessary for prompt diagnosis and appropriate management. One such condition is passenger lymphocyte syndrome (PLS. This case report describes the case of a 27-year-old male renal allograft recipient of the B-positive blood group who received a kidney from an O-positive donor. Postoperatively, the patient showed declining hemoglobin (Hb level and was transfused with B-group packed RBCs (PRBCs, following which there was steep fall in Hb level. A request for PRBCs was sent to the blood bank and this time cross-match with B-group PRBCs showed incompatibility. The patient′s RBCs were found to be strongly DAT (direct anti-globulin test positive and the eluate showed the presence of anti-B with a titer of 32. Thus, diagnosis of probable PLS was made. The patient was managed with methylprednisolone, plasmapheresis and O-group PRBCs. Gradually his condition improved and was discharged in stable condition.

  20. Seroprevalence of Helicobacter pyloriin school-aged Chinese in Taipei City and relationship between ABO blood groups

    Institute of Scientific and Technical Information of China (English)

    Tzee-Chung Wu; Liang-Kung Chen; Shinn-Jang Hwang

    2003-01-01

    AIM: To explore the seropositive rate of antibodies against H. pylori(anti-HP) in Taipei City and to compare the relationship of ABO blood groups and H. pylori infection.METHODS:In 1993, high school students in Shih-Lin District were randomly selected for blood samplings by their registration number at school. In addition, similar procedures were performed on the well-children clinics of Taipei Veterans General Hospital. Besides, randomly selected sera from the adults who took the physical examination were recruited for evaluation. Informed consents were obtained from all the subjects before blood samplings and parents were simultaneously informed for those who were younger than 18-year-old. Blood tests for anti-HP and ABO blood groupings were performed by enzyme-linked immunosorbent assay.Chi square tests were used for the comparisons between seroprevalence of H. pylori and ABO blood groups.RESULTS: Totally, 685 subjects were recruited (260 children aged 1-14 years, 425 high school students aged 15-18 years)were evaluated, and another 88 adult healthy volunteers were studied as well for comparison. The age-specific seropositive rate of anti-HP was 1.3 % at age 1-5 years,7.7 % at age 6-10 years, and 11.5 % at age 11-14 years.The seroprevalence of H. pylori infection was abruptly increased in young adolescence: 18.6 % at age 15 years,28.1% at age 16 years, 32.4 % at age 17 years and 41.0%at age 18 years, respectively. In the 425 high school students,ABO blood groupings were performed, which disclosed 48.5 % (206/425) of blood group O, 24 % (102/425) of blood group A, 21.8 % (93/425) of blood group B and 5.6 %(24/425) of blood group AB. In comparison of the subjects with blood group O and the other blood groups, no statistical significance could be identified in the seroprevalence of H. pylori(P=0.99).CONCLUSION: The seroprevalence of H. pylori infection in Taipei City in adults is similar to the developed countries,and the abrupt increase of H. pylori during high

  1. ABO blood groups and Helicobacter pylori cagA infection: evidence of an association

    Directory of Open Access Journals (Sweden)

    DE Mattos

    2010-01-01

    Full Text Available Diseases resulting from Helicobacter pylori infection appear to be dependent on a host of genetic traits and virulence factors possessed by this microorganism. This paper aimed to investigate the association between the ABO histo-blood groups and H. pylori cagA infections. Genomic DNA samples (n = 110 of gastric biopsies obtained from patients with endoscopic diagnosis of peptic ulcers (n = 25 and chronic active gastritis (n = 85 were analyzed by PCR using specific primers for the cagA gene. Of the samples, 66.4% (n = 73 tested positive and 33.6% (n = 37 negative for the gene. The cagA strain was predominant in peptic ulcers (n = 21; 84.0% compared with chronic active gastritis (n = 52; 61.2% (p = 0.05; OR 3.332; 95% CI: 1.050-10.576. Additionally, the cagA strain was prevalent in the type O blood (48/63; 76.2% compared with other ABO phenotypes (25/47; 53.2% (p = 0.01; OR 2.816; 95% CI: 1.246-6.364. These results suggest that H. pylori cagA infection is associated with the O blood group in Brazilian patients suffering from chronic active gastritis and peptic ulcers.

  2. Association of duffy blood group gene polymorphisms with IL8 gene in chronic periodontitis.

    Directory of Open Access Journals (Sweden)

    Emília Ângela Sippert

    Full Text Available The antigens of the Duffy blood group system (DARC act as a receptor for the interleukin IL-8. IL-8 plays an important role in the pathogenesis of chronic periodontitis due to its chemotactic properties on neutrophils. The aim of this study was to investigate a possible association of Duffy blood group gene polymorphisms with the -353T>A, -845T>C and -738T>A SNPs of the IL8 gene in chronic periodontitis. One hundred and twenty-four individuals with chronic periodontitis and 187 controls were enrolled. DNA was extracted using the salting-out method. The Duffy genotypes and IL8 gene promoter polymorphisms were investigated by PCR-RFLP. Statistical analyses were conducted using the Chi square test with Yates correction or Fisher's Exact Test, and the possibility of associations were evaluated by odds ratio with a 95% confidence interval. When analyzed separately, for the Duffy blood group system, differences in the genotype and allele frequencies were not observed between all the groups analyzed; and, in nonsmokers, the -845C allele (3.6% vs. 0.4%, -845TC genotype (7.3% vs. 0.7% and the CTA haplotype (3.6% vs. 0.4% were positively associated with chronic periodontitis. For the first time to our knowledge, the polymorphisms of erythroid DARC plus IL8 -353T>A SNPs were associated with chronic periodontitis in Brazilian individuals. In Afro-Brazilians patients, the FY*02N.01 with IL8 -353A SNP was associated with protection to chronic periodontitis.

  3. Rh, Kell, Duffy, Kidd and Diego blood group system polymorphism in Brazilian Japanese descendants.

    Science.gov (United States)

    Flôres, Marli Aparecida Luvisuto Rossett; Visentainer, Jeane Eliete Laguila; Guelsin, Gláucia Andréia Soares; Fracasso, Adriana de Souza; de Melo, Fabiano Cavalcante; Hashimoto, Margareth Naomi; Sell, Ana Maria

    2014-02-01

    Polymorphisms of Rh, Kell, Duffy, Kidd and Diego blood group systems were studied in 209 unrelated Brazilian Japanese descendants from South of Brazil. The methods used were multiplex-PCR, AS-PCR and RFLP-PCR. The differences in frequencies among the populations were evaluated using chi-square test. The frequencies for Rh, Kell, Kidd and Diego system were similar to those of the Japanese. RHCE(*)CC, RHCE(*)EE genotypes and FY(*)01 allele were lower and FY(*)01N.01 was higher than Japanese. These differences in the frequencies between Brazilian Japanese descendants and Japanese could indicate a gene flow in Brazilian population and reinforce the importance of this knowledge to achieve safe red blood cells. PMID:24231689

  4. Effects of A and B Wolbachia and host genotype on interspecies cytoplasmic incompatibility in Nasonia.

    Science.gov (United States)

    Bordenstein, S R; Werren, J H

    1998-04-01

    Wolbachia endosymbionts cause postmating reproductive isolation between the sibling species Nasonia vitripennis and N. giraulti. Most Nasonia are doubly infected with a representative from each of the two major Wolbachia groups (A and B). This study investigates the role of single (A or B) and double (A and B) Wolbachia infections in interspecies cytoplasmic incompatibility (CI) and host genomic influences on the incompatibility phenotype. Results show that the single A Wolbachia harbored in N. vitripennis (wAv) is bidirectionally incompatible with the single A Wolbachia harbored in N. giraulti (wAg). Results also indirectly show that the N. vitripennis wBv is bidirectionally incompatible with the N. giraulti wBg. The findings support current phylogenetic evidence that suggests these single infections have independent origins and were acquired via horizontal transfer. The wAv Wolbachia expresses partial CI in the N. vitripennis nuclear background. However, following genomic replacement by introgression, wAv expresses complete CI in the N. giraulti background and remains bidirectionally incompatible with wAg. Results show that double infections can reinforce interspecies reproductive isolation through the addition of incompatibility types and indicate that the host genome can influence incompatibility levels. This study has implications for host-symbiont coevolution and the role of Wolbachia in speciation.

  5. Sulphydryl groups and their relation to the antioxidant enzymes of chelonian red blood cells.

    Science.gov (United States)

    Torsoni, M A; Viana, R I; Ogo, S H

    1998-09-01

    Thiol groups of hemoglobin and blood glutathione are higher in Geochelone carbonaria than in Geochelone denticulata. Exposure of stripped hemolysate of both tortoises to terc-butyl hydroperoxide, resulted in a higher ferroheme oxidation of G. denticulata hemoglobin. In this example glutathione reductase and glutathione peroxidase, were not active due to the absence of GSH and NADPH, suggesting that the thiol groups of G. carbonaria hemoglobin act as antioxidant, similar to GSH. In the total hemolysate, however, where the antioxidant enzymes are active, both species showed similar levels of hemoglobin oxidation, suggesting that the protective effect of thiol groups of hemoglobin are less effective for heme protection. The activity of glutathione reductase and glutathione peroxidase was higher in erythrocytes of G. denticulata and the activity of catalase and superoxide dismutase was higher in erythrocytes of G. carbonaria. PMID:9784849

  6. Association of ABO and Rh blood groups to HBV, HCV infections among blood donors in a blood bank of tertiary care teaching hospital in Southern India: A retrospective study

    Directory of Open Access Journals (Sweden)

    Sreedhar Babu KV

    2015-07-01

    Conclusion: In this study conducted to determine the predominant blood group antigen and its association with HBV and HCV seroreactivity, there was no association between blood group antigens with these infections. [Int J Res Med Sci 2015; 3(7.000: 1672-1676

  7. Isolation of bifidobacteria for blood group secretor status targeted personalised nutrition

    Directory of Open Access Journals (Sweden)

    Harri Mäkivuokko

    2012-06-01

    Full Text Available Background: Currently, there is a constant need to find microbial products for maintaining or even improving host microbiota balance that could be targeted to a selected consumer group. Blood group secretor status, determining the ABO status, could be used to stratify the consumer group. Objective: We have applied a validated upper intestinal tract model (TIM-1 and culturing methods to screen potential probiotic bacteria from faeces of blood secretor and non-secretor individuals. Design: Faecal samples from healthy volunteers were pooled to age- and sex-matched secretor and non-secretor pools. Faecal pools were run through separate TIM-1 simulations, and bacteria were cultivated from samples taken at different stages of simulations for characterisation. Results: Microbes in secretor pool survived the transit through TIM-1 system better than microbes of non-secretor pool, especially bifidobacteria and anaerobes were highly affected. The differences in numbers of bifidobacteria and lactobacilli isolates after plate cultivations and further the number of distinct RAPD-genotypes was clearly lower in non-secretor pool than in secretor pool. Conclusions: In the present study, we showed that microbiota of secretor and non-secretor individuals tolerate gastrointestinal conditions differently and that a combination of gastrointestinal simulations and cultivation methods proved to be a promising tool for isolating potentially probiotic bacteria.

  8. Isolation of a very high molecular weight polylactosamine from an ovarian cyst mucin of blood group

    Energy Technology Data Exchange (ETDEWEB)

    Wu, A.S.S.; Bush, C.A.

    1986-05-01

    Treatment of a blood group A active ovarian cyst mucin glycoprotein with alkaline borohydride under conditions expected to cleave-O-glycosidically linked carbohydrate chains releases a polysaccharide of average molecular weight 25,000 daltons. It contains no peptide or mannose at the 1% level and carbohydrate analysis gives fuc:galNAc:gal:glcNAc in the ratio of 1:1:2.5:2.5. The /sup 13/C and /sup 1/H NMR spectra show that the polysaccharide has non-reducing terminal side chains of the structure galNAc(..cap alpha..-1 ..-->.. 3)(fuc(..cap alpha..-1 ..-->.. 2)) gal(..beta..-1 ..-->.. 3) glcNAc (i.e. a type 1 chain). Periodate oxidation removes all the fucose and galNAc from the non-reducing terminal but leaves intact the backbone composed of ..beta..-linked gal and glcNAc as would be expected for a polylactosamine. They conclude that this is a high molecular weight polylactosamine which is related to the asparagine linked polylactosamine chains of cell surface glycoproteins which have been implicated in cell differentiation. However, the blood group A polysaccharide from the ovarian cyst mucin is unique in several respects. It has a much larger molecular weight than even the erythroglycan of the red cell membrane protein, band 3, and is linked to the protein by an -O-glycosidic bond rather than the -N-asparagine linkage of the previously known polylactosamines which have a trimannosyl core. Its blood group A side chains are on a type one core rather than type 2 which is found on other polylactosamines.

  9. Isolation of a very high molecular weight polylactosamine from an ovarian cyst mucin of blood group

    International Nuclear Information System (INIS)

    Treatment of a blood group A active ovarian cyst mucin glycoprotein with alkaline borohydride under conditions expected to cleave-O-glycosidically linked carbohydrate chains releases a polysaccharide of average molecular weight 25,000 daltons. It contains no peptide or mannose at the 1% level and carbohydrate analysis gives fuc:galNAc:gal:glcNAc in the ratio of 1:1:2.5:2.5. The 13C and 1H NMR spectra show that the polysaccharide has non-reducing terminal side chains of the structure galNAc(α-1 → 3)[fuc(α-1 → 2)] gal(β-1 → 3) glcNAc (i.e. a type 1 chain). Periodate oxidation removes all the fucose and galNAc from the non-reducing terminal but leaves intact the backbone composed of β-linked gal and glcNAc as would be expected for a polylactosamine. They conclude that this is a high molecular weight polylactosamine which is related to the asparagine linked polylactosamine chains of cell surface glycoproteins which have been implicated in cell differentiation. However, the blood group A polysaccharide from the ovarian cyst mucin is unique in several respects. It has a much larger molecular weight than even the erythroglycan of the red cell membrane protein, band 3, and is linked to the protein by an -O-glycosidic bond rather than the -N-asparagine linkage of the previously known polylactosamines which have a trimannosyl core. Its blood group A side chains are on a type one core rather than type 2 which is found on other polylactosamines

  10. Outcome of consecutive pregnancies in a patient with Bombay (Oh) blood group.

    Science.gov (United States)

    Bhattacharya, S; Makar, Y; Laycock, R A; Gooch, A; Poole, J; Hadley, A

    2002-12-01

    A young lady with a rare Bombay (Oh) blood group had two successive uneventful pregnancies. Her serum contained a potent high-titre anti-H and serological as well as chemiluminescence tests, suggesting that the antibody was haemolytic. Her husband was of the normal H status. Theoretically, both babies should have been positive for the H antigen and should have suffered from haemolytic disease of the newborn. This apparent conundrum could be owing to the weak expression of the H antigens on the infant red cells.

  11. FREQUENCY AND DISTRIBUTION OF ABO & RH BLOOD GROUP IN BILASPUR DISTRICT OF CHHATTISGARH STATE : A STUDY FROM MEDICAL COLLEGE HOSPITAL

    Directory of Open Access Journals (Sweden)

    Bhanu Pratap

    2015-07-01

    Full Text Available BACKGROUND : Approximate 30 blood group systems have discovered and more than 400 erythrocytes antigens are identified. Blood group ABO and Rh are most important among all other blood group systems in transfusion service practices. The frequency of four major blood gr oup s namely A, B, O, AB with Rh Positive and Negative varies in different population of the world and differ also in region and race wise. MATERIAL AND METHOD : This 5 years retrospective study was conducted at Blood Bank of a Medical college Hospital of Bi laspur in Northern Chhattisgarh, catering the 1/3 population of state. Data were collected from the Blood Bank Grouping record from the period of January 2010 to December 2014. Blood group of blood donors and patients were determined by Monoclonal Anti Ser a by slide agglutinations tests. Rare case and difficult case were examined by test tube agglutination method and Matrix Gel System of Tulip. RESULT AND CONCLUSIO N: 31973 subjects were examined for blood group during observation period, Out of these 31092( 97.25% were male and 881 (2.75% were female. The frequency of blood group B in these populations was 11007 (34.42% (33.36% Rh Positive and 1.06% Rh Negative Followed by O were 10864 (33.97% (33.33% Rh Positive and 0.64% Rh Negative, A was 9113 (28.50 % (27.99 % Rh Positive and 0.51% Rh Negative and AB was 989 (3.11% (3.01% Rh Positive and 0.1% Rh Negative. Rhesus group Rh Positive were 31242 (97.7 % and Rh Negative were 731 (2.3 %.

  12. Identification of a rare blood group, "Bombay (Oh phenotype," in Bhuyan tribe of Northwestern Orissa, India

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    Balgir R

    2007-01-01

    Full Text Available Background: Blood group serology plays a vital role in transfusion medicine. The Bombay (Oh phenotype is characterized by the absence of A, B, and H antigens on red cells and occurs rarely, especially in tribal populations of India. Aims and Objectives: This is a field-based random population study in the Bhuyan tribal community. The study reports three cases of the rare Bombay (Oh phenotype for the first time in the Bhuyan tribe of Sundargarh district in North-Western Orissa. Materials and Methods: Taking informed consent, red blood cells of 836 Bhuyan subjects were tested with three antisera, i.e., anti-A, anti-B, and anti-H (lectin for forward reaction. Agglutinations of plasma with A, B, and O (H red cells (reverse reaction were also tested for the presence or absence of antibodies in the serum. Specialized tests like absorption-elution, titration of naturally occurring antibodies at different temperatures, inhibition of anti-H by O saliva secretor, and determination of secretor status were performed. Results: Three cases of a rare blood group, Bombay (Oh phenotype, (2 out of 244 Khandayat Bhuyan and 1 out of 379 Paudi Bhuyan from Hemgiri and Lahunipara blocks, respectively in the Bhuyan tribe of Sundargarh district in North-Western Orissa were detected, giving an incidence of 1 in 122 in Khandayat Bhuyan and 1 in 379 in Paudi Bhuyan, with an average of 1 in 278 among the Bhuyan tribal population. This incidence is high in comparison to earlier studies reported from India. Conclusions: The practice of tribal and territorial endogamy in a smaller effective populations (for example, there are only 3,521 individuals in Paudi Bhuyan results in smaller marital distance and inbreeding, leading to increased homozygous expression of rare recessive genetic characters like the Bombay (Oh phenotype. This study further testifies that the incidence is higher in those states of India where the consanguinity is a common practice.

  13. Extensive Adaptive Changes Occur in the Transcriptome of Streptococcus agalactiae (Group B Streptococcus) in Response to Incubation with Human Blood

    OpenAIRE

    Laurent Mereghetti; Izabela Sitkiewicz; Nicole M Green; Musser, James M.

    2008-01-01

    To enhance understanding of how Streptococcus agalactiae (group B streptococcus, GBS) adapts during invasive infection, we performed a whole-genome transcriptome analysis after incubation with whole human blood. Global changes occurred in the GBS transcriptome rapidly in response to blood contact following shift from growth in a rich laboratory medium. Most (83%) of the significantly altered transcripts were down-regulated after 30 minutes of incubation in blood, and all functional categories...

  14. Expression of Lewisb blood group antigen in Helicobacterpylori does not interfere with bacterial adhesion property

    Institute of Scientific and Technical Information of China (English)

    Peng-Yuan Zheng; Jiesong Hua; Han-Chung Ng; Khay-Guan Yeoh; Ho Bow

    2003-01-01

    AIM: The finding that some Helicobacterpyloristrains expressLewis b (Leb) blood group antigen casts a doubt on the roleof Leb of human gastric epithelium being a receptor for-H.pylori. The aim of this study was to determine if expressionof Leb in H. Pyloriinterferes with bacterial adhesion property.METHODS: Bacterial adhesion to immobilized Leb onmicrotitre plate was performed in 63-H. Pyloristrains obtainedfrom Singapore using in vitro adherence assay. Expression ofLewis blood group antigens was determined by ELISA assay.RESULTS: Among 63 H. Pyloristrains, 28 expressed Lebantigen. In vitro adhesion assay showed that 78.6 % (22/28) of Leb-positive and 74.3 % (26/35) of Leb-negative-H.pyloriisolates were positive for adhesion to immobilized Lebcoated on microtitre plate (P=0.772). In addition, blockingof H. Pylori Leb by prior incubation with anti-Leb monoclonalantibody did not alter thebinding of the bacteria to solid-phase coated Leb.CONCLUSION: The present study suggests that expressionof Leb in H. Pyloridoes not interfere with the bacterialadhesion property. This result supports the notion that Lebpresent on human gastric epithelial cells is capable of beinga receptor for H.pylori.

  15. A modified PCR-SSP method for the identification of ABO blood group antigens.

    Science.gov (United States)

    Downing, J; Darke, C

    2003-08-01

    The ABO blood group antigens are carbohydrate molecules synthesized by the glycosyltransferases encoded by the ABO gene on chromosome 9. Kidney transplantation across the ABO barrier generally leads to rapid humoral graft rejection due to the presence of naturally occurring antibodies to the A and B antigens. We have developed a method for ABO typing our cadaveric organ donors by the polymerase chain reaction using sequence-specific primers (PCR-SSP). The method uses 12 primers in eight PCR mixtures and is performed under the same conditions as our routine HLA-A, B, C PCR-SSP typing. The PCR-SSP-based types of 166 regular blood donors and 148 cadaveric organ donors all showed total concordance with their serologically assigned ABO groups. Six individuals possessing the ABO A subgroups (A3, Ax and Aend) all typed as A1 by PCR-SSP, as expected. PCR-SSP is an appropriate method for ABO typing of cadaveric organ donors and, importantly, enables both ABO and HLA typing to be performed on the same DNA material.

  16. [Alanine solution as enzyme reaction buffer used in A to O blood group conversion].

    Science.gov (United States)

    Li, Su-Bo; Zhang, Xue; Zhang, Yin-Ze; Tan, Ying-Xia; Bao, Guo-Qiang; Wang, Ying-Li; Ji, Shou-Ping; Gong, Feng; Gao, Hong-Wei

    2014-06-01

    The aim of this study was to investigate the effect of alanine solution as α-N-acetylgalactosaminidase enzyme reaction buffer on the enzymatic activity of A antigen. The binding ability of α-N-acetylgalactosaminidase with RBC in different reaction buffer such as alanine solution, glycine solution, normal saline (0.9% NaCl), PBS, PCS was detected by Western blot. The results showed that the efficiency of A to O conversion in alanine solution was similar to that in glycine solution, and Western blot confirmed that most of enzymes blinded with RBC in glycine or alanine solution, but few enzymes blinded with RBC in PBS, PCS or normal saline. The evidences indicated that binding of enzyme with RBC was a key element for A to O blood group conversion, while the binding ability of α-N-acetylgalactosaminidase with RBC in alanine or glycine solution was similar. It is concluded that alanine solution can be used as enzyme reaction buffer in A to O blood group conversion. In this buffer, the α-N-acetylgalactosaminidase is closely blinded with RBC and α-N-acetylgalactosaminidase plays efficient enzymatic activity of A antigen.

  17. 犬血型与安全输血研究进展%Research Advance in Canine Blood Group and Safe Blood Transfusion

    Institute of Scientific and Technical Information of China (English)

    丁晓麟; 温海; 贺星亮; 张汇东

    2011-01-01

    This paper reviews the research progress in the safe blood transfusion of dog, including canine blood group system, identification of blood group, cross matching, selection of the donor, transfusion reaction and so on, systematically explores how to conduct the safe and effective blood transfusion of dog, and discusses the current existent problems.%综述了犬血型系统、血型鉴定、交叉配血、供血犬选择以及输血反应等与安全输血相关的各方面的研究进展,系统探讨了临床上如何对犬进行安全、有效输血以及当前存在问题.

  18. Des loyautés incompatibles

    Directory of Open Access Journals (Sweden)

    Daniel Bizeul

    2007-06-01

    Full Text Available Faire l’expérience directe des phénomènes, en participant aux activités des milieux concernés, représente un des moyens les plus naturels et les plus sûrs pour atteindre la complexité du monde social. C’est aussi un des moyens les plus perturbants pour le chercheur, souvent confronté aux interdits de contact entre des groupes dont les intérêts ou les principes de vie sont antagonistes. Établir des relations étroites avec des militants du Front national, certains d’entre eux « néo-nazis », certains vivant à quelques rues de chez soi, tout en étant « homo », avec pour proches amis des hommes de couleur, de plus entouré de personnes hostiles à ce parti, oblige ainsi à gérer des liens inconciliables. Un risque possible pour le chercheur est alors d’être soumis à des sentiments contradictoires et de mettre du temps pour aboutir à une analyse exempte de toute influence indue.Incompatible loyalties. Moral aspects of an immersion into the French National Front Party.Experiencing directly phenomenon while participating to activities of the concerned circles, represents one of the most natural and safer way to reach the complexity of the society. It is also one of the most disturbing means for the researcher, often confronted to interdicts of contacts between groups which interests or principles of life are antagonists. Creating close relationships with French National Front party activists, some of them “neo-nazis”, some of them living a few streets from your house, while being gay, with colored men as closed friends, surrounded by persons hostile to this party, thus forces to make opposed connections. A possible risk for the researcher can be to be divided between contradictory feelings and spending time finishing an analysis free of unwarranted influence.Lealtades incompatibles. Problemas morales derivados de los contactos con el « Frente nacional »Establecer un contacto directo con fenómenos de diferente

  19. Research progress of techniques of blood grouping for red blood cells%红细胞血型鉴定技术的研究进展

    Institute of Scientific and Technical Information of China (English)

    张强

    2015-01-01

    Blood grouping is one of the methods to confirm red blood cell grouping.For over a century,the hemagglutination of red blood cells has been the main method for blood phenotyping in clinical practices.With the development of molecular diagnostic techniques,there are many genotyping methods used in red blood cell grouping now,such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP),PCR-sequence specific primer (SSP),multiple PCR,PCR-enzyme-linked immunosorbent assay (ELISA),DNA sequencing and gene chip and so on.Compared with serology,the new techniques of blood grouping for red blood cells based on genotype may be more advantageous in throughput,time-consumption and cost.This review will summarize the techniques of blood grouping for red blood cells,aiming at providing a systematic knowledge of blood grouping techniques for clinicians.%血型鉴定是对红细胞血型进行确认的一种方法,近一个多世纪以来,血细胞凝集反应成为血型鉴定的主要手段.随着分子诊断技术的发展,已诞生多种红细胞血型鉴定的基因检测分型技术,如限制性酶切片段长度多态性聚合酶链反应(PCR-RFLP)、序列特异性引物PCR(PCR-SSP)、多重PCR、适时定量PCR、PCR-酶联免疫吸附试验(ELISA)、DNA测序及基因芯片等.有关红细胞血型鉴定的新技术在检测通量、速度、便捷性及检测成本方面,均比血清学方法更具优势.笔者拟就红细胞血型鉴定技术的研究进展进行综述,旨在让临床医师对目前红细胞血型鉴定技术有一个系统性认识.

  20. EFFECT OF PLANT LECTINS ON HUMAN BLOOD GROUP ANTIGENS WITH SPECIAL FOCUS ON PLANT FOODS AND JUICES

    Directory of Open Access Journals (Sweden)

    B. Venkata Raman

    2012-04-01

    Full Text Available Different plant lectins have been studied for lectin binding activity on ABO blood group system individually to study their suitability for consumption. 45% of plants were found to show blood group agglutination activity against A, B, AB and O groups. These results showed more suitability for consumption of investigated plants and their products to entire human population. Data also alarming human to be more careful about the plant lectins reacting with blood groups as the similar reactions may possibly happen at mucosal surface of the gut. In fact, chemical composition on RBC may similar with mucosal cell surfaces of human gastrointestinal tract. In our investigation results reveal that 27 percent of plant extracts showed activity against A, 38 percent of plant extracts for B, 45 percent plant extracts on AB and 45 percent of plants on O group blood populations of human beings. Further, O blood group humans have shown more significant activity (10 different plants than A, B and AB. Hence, these double blind placebo studies are very promising and would give better results for suitability and digestibility of foods taking either as staple foods or juices, and also several health benefits for controlling the diet intake, based on the blood group type.

  1. The abundance and organization of polypeptides associated with antigens of the Rh blood group system.

    Science.gov (United States)

    Gardner, B; Anstee, D J; Mawby, W J; Tanner, M J; von dem Borne, A E

    1991-06-01

    Twelve murine monoclonal antibodies, which react with human red cells of common Rh phenotype but give weak or negative reactions with Rh null erythrocytes, were used in quantitative binding assays and competitive binding assays to investigate the abundance and organization of polypeptides involved in the expression of antigens of the Rh blood group system. Antibodies of the R6A-type (R6A, BRIC-69, BRIC-207) and the 2D10-type (MB-2D10, LA18.18, LA23.40) recognize related structures and 100,000-200,000 molecules of each antibody bind maximally to erythrocytes of common Rh phenotype. Antibodies of the BRIC-125 type (BRICs 32, 122, 125, 126, 168, 211) recognize structures that are unrelated to those recognized by R6A-type and 2D10-type antibodies and between 10,000 and 50,000 antibody molecules bind maximally to erythrocytes of the common Rh phenotype. The binding of antibodies of the R6A-type and the 2D10-type, but not of antibodies of the BRIC-125-type could be partially inhibited by human anti-D antibodies (polyclonal and monoclonal) and a murine anti-e-like antibody. These results are consistent with evidence (Moore & Green 1987; Avent et al., 1988b) that the Rh blood group antigens are associated with a complex that comprises two groups of related polypeptides of M(r) 30,000 and M(r) 35,000-100,000, respectively, and suggest that there are 1-2 x 10(5) copies of this complex per erythrocyte. The polypeptide recognized by antibodies of the BRIC-125 type is likely to be associated with this complex. PMID:9259831

  2. The abundance and organization of polypeptides associated with antigens of the Rh blood group system.

    Science.gov (United States)

    Gardner, B; Anstee, D J; Mawby, W J; Tanner, M J; von dem Borne, A E

    1991-06-01

    Twelve murine monoclonal antibodies, which react with human red cells of common Rh phenotype but give weak or negative reactions with Rh null erythrocytes, were used in quantitative binding assays and competitive binding assays to investigate the abundance and organization of polypeptides involved in the expression of antigens of the Rh blood group system. Antibodies of the R6A-type (R6A, BRIC-69, BRIC-207) and the 2D10-type (MB-2D10, LA18.18, LA23.40) recognize related structures and 100,000-200,000 molecules of each antibody bind maximally to erythrocytes of common Rh phenotype. Antibodies of the BRIC-125 type (BRICs 32, 122, 125, 126, 168, 211) recognize structures that are unrelated to those recognized by R6A-type and 2D10-type antibodies and between 10,000 and 50,000 antibody molecules bind maximally to erythrocytes of the common Rh phenotype. The binding of antibodies of the R6A-type and the 2D10-type, but not of antibodies of the BRIC-125-type could be partially inhibited by human anti-D antibodies (polyclonal and monoclonal) and a murine anti-e-like antibody. These results are consistent with evidence (Moore & Green 1987; Avent et al., 1988b) that the Rh blood group antigens are associated with a complex that comprises two groups of related polypeptides of M(r) 30,000 and M(r) 35,000-100,000, respectively, and suggest that there are 1-2 x 10(5) copies of this complex per erythrocyte. The polypeptide recognized by antibodies of the BRIC-125 type is likely to be associated with this complex.

  3. Action of glycosyl transferases upon "Bombay" (Oh) erythrocytes. Conversion to cells showing blood-group H and A specificities.

    Science.gov (United States)

    Schenkel-Brunner, H; Prohaska, R; Tuppy, H

    1975-08-15

    Individuals of the rare "Bombay" (Oh) blood-group phenotype lacking, due to a genetic defect, the alpha(1-2)fucosyl transferase, which is responsible for converting blood-group H precursor substances to H-specific structures. Treatment with GDP-fucose and alpha(1-2)fucosyl transferase prepared from gastric mucosa of O individuals to transform native or ficin-treated "Bombay" erythrocytes into cells phenotypically resembling O cells. The transformation was achieved, however, after prior incubation of the "Bombay" erythrocytes with neuraminidase, indicating that blood-group H precursor molecules on the surface of these cells are masked by sialyl residues. Blood-group A specificity was conferred upon neuraminidase-treated "Bombay" cells by enzymatic transfer of alpha-N-acetylgalactosamine residues, in addition to alpha-fucose residues.

  4. Protoplasmic Incompatibility in PODOSPORA ANSERINA: a Possible Function for Incompatibility Genes

    OpenAIRE

    Boucherie, Hélian; Bernet, Jean

    1980-01-01

    The suppression of protoplasmic incompatibility resulting from nonallelic gene interactions has been obtained by the coupled effect of mutations in the modA and modB genes (Bernet 1971). Due to their female sterility, modA modB strains provide an experimental tool to determine whether or not the mod and incompatibility loci are involved in a function other than protoplasmic incompatibility. Present results show that modA modB female sterility is a nonautonomous trait since heterokaryotic myce...

  5. 26. Relationship between ABO Blood Groups and Carcinoma of Esophagus and Cardia in Chaoshan Littoral of China

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Abstract: Background. Chaoshan is the only littoral among the six high-risk areas of esophageal carcinoma (EC) in China and the relatively isolatal society for the inconvenient transport gives an opportunity to study the genetics of carcinoma of esophagus and cardia. Some reports had suggested that ABO blood groups were associated with tumors, but their relation remained controversial. Methods: The data of age, sex, ABO blood type and X-ray or pathological diagnosis of the patients with carcinoma of esophagus or cardia were collected from The tumor hospital, First affiliated hospital, Second affiliated hospital of Shantou University Medical College; the Center hospital of Shantou and the Center hospital of Jieyang. In this study, 6 685 patients with EC and 2 955 patients with cardiac cancer(CC) in Chaoshan district were retrospectively assessed the association with ABO blood groups. Results: The distribution of ABO blood groups in patients with EC or CC was similar to the normal local population in Chaoshan. However, there was 2.3% excess of blood group B in male patients with CC and 4.7% excess in the patients with carcinoma in the upper third esophagus, compared with the corresponding controls. The relative risk B:O was 1.1415 (P<0.05) and 1.2696 (P<0.05), respectively No relationship between ABO blood groups and tumor differentiation was found. Conclusion: ABO blood group B was associated with the incidence of CC in male individuals and carcinoma in the upper third esophagus. The distribution of ABO blood groups varied in the different geographical and ethnic groups, as a result, proper controls were very important for such study.

  6. Simultaneous forward and reverse ABO blood group typing using a paper-based device and barcode-like interpretation.

    Science.gov (United States)

    Songjaroen, Temsiri; Laiwattanapaisal, Wanida

    2016-05-19

    A new platform of a paper-based analytical device (PAD) for simultaneous forward and reverse ABO blood group typing has been reported. This platform can overcome the discrepancy results as influenced by the individual haematocrit. The test and the control of non-haemagglutination on each channel were performed in parallel. The PAD was fabricated by printing six parallel channels with wax onto Whatman No. 4 filter paper. An LF1 blood separation membrane was used for the separation of plasma from whole blood for reverse grouping. The blood group was identified by haemagglutination of the corresponding antigen-antibody. For forward grouping, Anti-A, -B and -A,B were treated on the test line of PAD, and inactivated Anti-A, -B and -A,B were immobilized on the control line. For reverse grouping, 30% standard A-cells, B- and O- were added to the test channel after plasma separation, and O-cells were used as a control. Then, 0.9% normal saline (NSS) containing 1% Tween-20 was bi-functionally used for dilution of the blood sample and elution of the non-agglutinated RBCs within the channels. The distance of agglutinated RBCs in each test line was compared with the distance of non-agglutinated RBCs in the parallel control line. The forward and reverse patterns of blood groups A, B, AB and O were a barcode-like chart in which the results can be visually analysed. The PAD has excellent reproducibility when 10 replications of the A, B, AB or O blood groups were performed. The results of both forward and reverse grouping were highly correlated with conventional methods compared with the slide method and tube method, respectively (n = 76). Thus, this ABO typing PAD holds great potential for future applications in blood typing point-of-care testing. PMID:27126791

  7. Incompatible Sets of Gradients and Metastability

    Science.gov (United States)

    Ball, J. M.; James, R. D.

    2015-12-01

    We give a mathematical analysis of a concept of metastability induced by incompatibility. The physical setting is a single parent phase, just about to undergo transformation to a product phase of lower energy density. Under certain conditions of incompatibility of the energy wells of this energy density, we show that the parent phase is metastable in a strong sense, namely it is a local minimizer of the free energy in an L 1 neighbourhood of its deformation. The reason behind this result is that, due to the incompatibility of the energy wells, a small nucleus of the product phase is necessarily accompanied by a stressed transition layer whose energetic cost exceeds the energy lowering capacity of the nucleus. We define and characterize incompatible sets of matrices, in terms of which the transition layer estimate at the heart of the proof of metastability is expressed. Finally we discuss connections with experiments and place this concept of metastability in the wider context of recent theoretical and experimental research on metastability and hysteresis.

  8. Widespread Genomic Incompatibilities in Caenorhabditis elegans

    NARCIS (Netherlands)

    Snoek, L.B.; Orbidans, H.E.; Stastna, J.; Aartse, A.; Rodriguez Sanchez, M.; Riksen, J.A.G.; Kammenga, J.E.; Harvey, S.C.

    2014-01-01

    In the Bateson-Dobzhansky-Muller (BDM) model of speciation, incompatibilities emerge from the deleterious interactions between alleles that are neutral or advantageous in the original genetic backgrounds, i.e. negative epistatic effects. Within species such interactions are responsible for outbreedi

  9. Incompatibilities analysis in the accredited laboratory

    Directory of Open Access Journals (Sweden)

    D. Szewieczek

    2008-06-01

    Full Text Available Purpose of the presented paper aimed at motivating the necessity of the accreditation of research and standardising laboratories as factors deciding about the competitive advantage of those organisations on the European Union market.Design/methodology/approach used for the research has covered the analyses of results of internal and external audits conducted in one of Polish accredited laboratories and estimation of the incompatibilities occurred.Findings of the carried out research are as follows: number and character of incompatibilities, which are exposed during internal and external audits, reflect size of organisation, where the management system is implemented, phase of implementation as well as the time of functioning.Practical implications refers to any organisation which has quality management system implemented as well as to any accredited laboratory using internal audits as an element of continuous improvement and treating incompatibilities not as something disqualifying the investigated area, but as an supporting element. Originality/value of the presented paper belongs to the methodology comprising the usage of internal audits’ results - proved incompatibilities - as a tool for obtaining and assuring the confidence in the management system.

  10. Are the blood groups of women with preeclampsia a risk factor for the development of hypertension postpartum?

    Directory of Open Access Journals (Sweden)

    Avci D

    2016-04-01

    Full Text Available Deniz Avci,1 Hatice Karagoz,2 Ozerhan Ozer,1 Kubra Esmeray,1 Kadir Bulut,1 Fatma Aykas,1 Ali Cetinkaya,1 Emine Uslu,1 Samet Karahan,1 Mustafa Basak,1 Abdulsamet Erden1 1Internal Medicine Department, Kayseri Training and Research Hospital, 2Internal Medicine Department, Acibadem Kayseri Hospital, Kayseri, Turkey Introduction: Preeclampsia (PE is a pregnancy-related disorder characterized by hypertension (HT and proteinuria noticeable after 20 weeks of gestation. PE is now considered as a cardiovascular disease risk factor and a number of studies have shown that experiencing PE increases the prevalence of various cardiovascular risk factors, such as metabolic syndrome and HT. In this study, we aimed to investigate any possible relationship between the ABO/Rh blood group system and PE in Turkey. In the second part of the study, we examined the relationship between the ABO blood group system and development of HT after PE. Patients and methods: A total of 250 patients with PE from Kayseri Training and Research Hospital between 2002 and 2012 were included in the study. Patients were classified according to blood groups (A, B, AB, and O and Rh status (+/-. Results: There was a significant difference between the patients with PE and the control group in terms of distribution of ABO blood groups and the percentage of group AB was found to be higher in patients with PE compared to the control group (P=0.029. The risk of developing PE was significantly higher in group AB than other blood groups (P=0.006. The risk of developing HT after PE was significantly higher in group O than other blood groups (P=0.004. Discussion: In this study, we found that the patients with blood group AB have a higher risk for PE. The patients with PE of blood group O are at high risk of developing HT, and Rh factor was identified as another risk at this point and these patients should be closely followed postpartum. Keywords: ABO blood groups, Rh factor, preeclampsia

  11. Hemagglutination inhibition studies of water soluble blood group substances recovered from the erythrocytes of classical Bombay Oh subjects.

    Science.gov (United States)

    Vos, G H; Moores, P P

    1976-01-01

    Using ethanol and acetone fractionation to isolate soluble blood group substances from red blood cells, 'Bombay' Oh bloods were found to contain variable amounts of concealed H substance. The IgG variety of anti-H in 'Bombay' bloods has a greater affinity for these substances than the IgM variety of anti-H. Group O parents of 'Bombay' Oh subjects were found to have normal levels of H substance, indicating that individuals heterozygous for a recessive suppressor gene 'x' synthesize it normally. In the 'Bombay' family studied, Lewis determinants were abnormally expressed in two members. Lewis activity was detected in the soluble extracts of their red blood cells but not by the direct agglutination test. Further tests using known Le(a-b-) types are necessary to determine whether these findings are linked to the 'Bombay' Oh phenomenon.

  12. Relationship between ABO blood group and pregnancy complications: a systematic literature analysis

    Science.gov (United States)

    Franchini, Massimo; Mengoli, Carlo; Lippi, Giuseppe

    2016-01-01

    Given the expression of ABO blood group antigens on the surface of a wide range of human cells and tissues, the putative interplay of the ABO system in human biology outside the area of transfusion and transplantation medicine constitutes an intriguing byway of research. Thanks to evidence accumulated over more than 50 years, the involvement of the ABO system in the pathogenesis of several human diseases, including cardiovascular, infectious and neoplastic disorders, is now acknowledged. However, there is controversial information on the potential association between ABO blood type and adverse pregnancy outcomes, including pre-eclampsia and related disorders (eclampsia, HELLP syndrome and intrauterine growth restriction), venous thromboembolism, post-partum haemorrhage and gestational diabetes. To elucidate the role of ABO antigens in pregnancy-related complications, we performed a systematic review of the literature published in the past 50 years. A meta-analytical approach was also applied to the existing literature on the association between ABO status and pre-eclampsia. The results of this systematic review are presented and critically discussed, along with the possible pathogenic implications. PMID:27177402

  13. Structural Constraints on Human Norovirus Binding to Histo-Blood Group Antigens.

    Science.gov (United States)

    Singh, Bishal K; Leuthold, Mila M; Hansman, Grant S

    2016-01-01

    Human norovirus interacts with the polymorphic human histo-blood group antigens (HBGAs), and this interaction is thought to be important for infection. The genogroup II genotype 4 (GII.4) noroviruses are the dominant cluster, evolve every other year, and are thought to modify their binding interactions with different HBGA types. Most human noroviruses bind HBGAs, while some strains were found to have minimal or no HBGA interactions. Here, we explain some possible structural constraints for several noroviruses that were found to bind poorly to HBGAs by using X-ray crystallography. We showed that one aspartic acid was flexible or positioned away from the fucose moiety of the HBGAs and this likely hindered binding, although other fucose-interacting residues were perfectly oriented. Interestingly, a neighboring loop also appeared to influence the loop hosting the aspartic acid. These new findings might explain why some human noroviruses bound HBGAs poorly, although further studies are required. PMID:27303720

  14. Lichenoid reaction associated with silver amalgam restoration in a Bombay blood group patient: A case report.

    Science.gov (United States)

    Pawar, Rohini Rangarao; Mattigatti, Sudha S; Mahaparale, Rushikesh R; Kamble, Amit P

    2016-01-01

    The pathogenic relationship between the oral lichenoid reaction (OLR) and dental restorative materials has been confirmed many times. An OLR affecting oral mucosa in direct contact with an amalgam restoration represents a delayed, type IV, cell mediated immune response to mercury or one of the other constituents of the dental amalgam. Bombay blood group patients are more prone to this. A case of bilateral OLR is presented, which is present in relation to amalgam restoration. The lesion healed up after the replacement of restorations with an intermediate restorative material. The clinician should be aware of all the possible pathological etiologies of white lesions. If there is any doubt about the nature or management of a usual oral lesion, a referral to an appropriate specialist is mandatory. PMID:27217647

  15. Lichenoid reaction associated with silver amalgam restoration in a Bombay blood group patient: A case report

    Directory of Open Access Journals (Sweden)

    Rohini Rangarao Pawar

    2016-01-01

    Full Text Available The pathogenic relationship between the oral lichenoid reaction (OLR and dental restorative materials has been confirmed many times. An OLR affecting oral mucosa in direct contact with an amalgam restoration represents a delayed, type IV, cell mediated immune response to mercury or one of the other constituents of the dental amalgam. Bombay blood group patients are more prone to this. A case of bilateral OLR is presented, which is present in relation to amalgam restoration. The lesion healed up after the replacement of restorations with an intermediate restorative material. The clinician should be aware of all the possible pathological etiologies of white lesions. If there is any doubt about the nature or management of a usual oral lesion, a referral to an appropriate specialist is mandatory.

  16. Lichenoid reaction associated with silver amalgam restoration in a Bombay blood group patient: A case report.

    Science.gov (United States)

    Pawar, Rohini Rangarao; Mattigatti, Sudha S; Mahaparale, Rushikesh R; Kamble, Amit P

    2016-01-01

    The pathogenic relationship between the oral lichenoid reaction (OLR) and dental restorative materials has been confirmed many times. An OLR affecting oral mucosa in direct contact with an amalgam restoration represents a delayed, type IV, cell mediated immune response to mercury or one of the other constituents of the dental amalgam. Bombay blood group patients are more prone to this. A case of bilateral OLR is presented, which is present in relation to amalgam restoration. The lesion healed up after the replacement of restorations with an intermediate restorative material. The clinician should be aware of all the possible pathological etiologies of white lesions. If there is any doubt about the nature or management of a usual oral lesion, a referral to an appropriate specialist is mandatory.

  17. Lichenoid reaction associated with silver amalgam restoration in a Bombay blood group patient: A case report

    Science.gov (United States)

    Pawar, Rohini Rangarao; Mattigatti, Sudha S.; Mahaparale, Rushikesh R.; Kamble, Amit P.

    2016-01-01

    The pathogenic relationship between the oral lichenoid reaction (OLR) and dental restorative materials has been confirmed many times. An OLR affecting oral mucosa in direct contact with an amalgam restoration represents a delayed, type IV, cell mediated immune response to mercury or one of the other constituents of the dental amalgam. Bombay blood group patients are more prone to this. A case of bilateral OLR is presented, which is present in relation to amalgam restoration. The lesion healed up after the replacement of restorations with an intermediate restorative material. The clinician should be aware of all the possible pathological etiologies of white lesions. If there is any doubt about the nature or management of a usual oral lesion, a referral to an appropriate specialist is mandatory. PMID:27217647

  18. Heterogeneity and diversity of ABO and Rh blood group genes in select Saudi Arabian populations.

    Science.gov (United States)

    AlSuhaibani, E S; Kizilbash, N A; Malik, S

    2015-01-01

    In order to investigate the diversity of ABO and Rh blood group genes in the Saudi Arabian population, we assembled the phenotypic data of approximately 66,000 subjects from ten representative Saudi populations: Al-Khobar, Riyadh, Tabuk/Madina Al-Munawaara, Jeddah, Abha, South region, Sakaka, Domah, Al-Qurayat, and Sweer. The frequencies of p[A], q[B], and r[O] alleles at the ABO locus were observed to be 0.1688, 0.1242, and 0.7070, respectively, and the frequency of the D allele at the Rh locus was 0.7138. The heterozygosities at the ABO and Rh loci were 0.4563 and 0.4086, respectively, while the combined heterozygosity was 0.4324. Homogeneity tests revealed the population of Abha to be the most heterogeneous while that of Tabuk/Madina was found to be the least heterogeneous. Homogeneity was higher among the Northern populations while Southern populations demonstrated subdivisions and stratification. Gene diversity analyses yielded a total heterozygosity value of 0.4449. The coefficient of gene differentiation was 0.0090. Nei's genetic distance analyses showed that there was close affinity between the populations of Al-Khobar and Riyadh. The largest differences were observed between the populations of Sakaka and Domah. Furthermore, negative correlations were found between p[A] and r[O] alleles, and between q[B] and r[O] alleles at the ABO locus. Clinal analyses revealed that the r[O] allele showed an increasing trend from North-East to South-West, and conversely the q[B] allele exhibited a decreasing trend at these coordinates. These analyses present interesting aspects of the blood group allele distribution across the geography of Saudi Arabia. PMID:26214466

  19. Structural analysis of the RH-like blood group gene products in nonhuman primates

    Energy Technology Data Exchange (ETDEWEB)

    Salvignol, I. [Centre Regional de Transfusion Sanguine, Toulouse (France); Calvas, P.; Blancher, A. [Universitaire d`Immunogenetique moleculaire, Toulouse (France); Socha, W.W. [University Medical Center, New York, NY (United States); Colin, Y.; Le Van Kim, C.; Bailly, P.; Cartron, J.P. [Institut National de la Transfusion Sanguine, Paris (France); Ruffie, J.; Blancher, A. [College de France, Paris (France)

    1995-03-01

    Rh-related transcripts present in bone marrow samples from several species of nonhuman primates (chimpanzee, gorilla, gibbon, crab-eating macaque) have been amplified by RT-polymerase chain reaction using primers deduced from the sequence of human RH genes. Nucleotide sequence analysis of the nonhuman transcripts revealed a high degree of similarity to human blood group Rh sequences, suggesting a great conservation of the RH genes throughout evolution. Full-length transcripts, potentially encoding 417 amino acid long proteins homologous to Rh polypeptides, were characterized, as well as mRNA isoforms which harbored nucleotide deletions or insertions and potentially encode truncated proteins. Proteins of 30-40,000 M{sub r}, immunologically related to human Rh proteins, were detected by western blot analysis with antipeptide antibodies, indicating that Rh-like transcripts are translated into membrane proteins. Comparison of human and nonhuman protein sequences was pivotal in clarifying the molecular basis of the blood group C/c polymorphism, showing that only the Pro103Ser substitution was correlated with C/c polymorphism. In addition, it was shown that a proline residue at position 102 was critical in the expression of C and c epitopes, most likely by providing an appropriate conformation of Rh polypeptides. From these data a phylogenetic reconstruction of the RH locus evolution has been calculated from which an unrooted phylogenetic tree could be proposed, indicating that African ape Rh-like genes would be closer to the human RhD gene than to the human RhCE gene. 55 refs., 4 figs., 1 tab.

  20. [Control of fertilization by self-incompatibility mechanisms].

    Science.gov (United States)

    Fobis-Loisy, Isabelle; Gaude, Thierry

    2010-01-01

    Flowering plants (angiosperms) are the most prevalent and evolutionarily advanced group of plants. Reproductive strategies that promote cross-fertilization have played an essential role in the success of angiosperms as they contribute to genetic variability among plant species. A major genetic barrier to self-fertilization is self-incompatibility (SI), which allows female reproductive cells to discriminate between self- and non-self pollen and specifically reject self-pollen. In this review, we describe three SI mechanisms showing that different flowering plant families use distinct molecules for recognition of self as well as diverse biochemical pathways to arrest pollen tube growth.

  1. The use of hirudin as universal anticoagulant in haematology, clinical chemistry and blood grouping.

    Science.gov (United States)

    Menssen, H D; Melber, K; Brandt, N; Thiel, E

    2001-12-01

    Undesirable interactions between anticoagulants and diagnostic test kit procedures so far have prevented the development of a single uniform blood sampling tube. Contrary to K2-EDTA, heparin and other anticoagulants, hirudin only minimally alters blood cells and dissolved blood constituents, thus qualifying as a universal anticoagulant for diagnostic purposes. Automated complete blood counts, automated analyses of clinical chemistry analytes and immunohaematology were performed from hirudinised and routinely processed blood obtained from healthy volunteers (n=35) and hospitalised patients (n=45). Hirudin (400 ATU/ml blood) sufficiently anticoagulated blood for diagnostic purposes. The measurements of automated complete blood counts obtained from K2-EDTA-anticoagulated and hirudinised blood correlated significantly as did the measurements of 24 clinical chemistry analytes from hirudinised plasma and serum. Regression analysis revealed that the results of complete blood counts and clinical chemistry tests were predictable from the respective measurements from hirudinised blood (p=0.001). Immunohaematological tests and cross-matching from hirudinised and native blood of the same donors gave identical results. Single clotting factors, but not global coagulation analytes, could be measured from hirudinised blood. Therefore, a universal hirudin-containing blood sampling tube could be designed for automated analysis of haematological, serological and clinical chemistry analytes. PMID:11798089

  2. Phenotype frequencies of blood group systems (Rh, Kell, Kidd, Duffy, MNS, P, Lewis, and Lutheran in blood donors of south Gujarat, India

    Directory of Open Access Journals (Sweden)

    Manoj A Kahar

    2014-01-01

    Full Text Available Background: This is the first study on phenotype frequencies of various blood group systems in blood donors of south Gujarat, India using conventional tube technique. Material and Methods: A total of 115 "O" blood group donors from three different blood banks of south Gujarat were typed for D, C, c, E, e, K, Jk a , Le a , Le b , P 1 , M, and N antigens using monoclonal antisera and k, Kp a , Kp b , Fy a ,Fy b , Jk b , S,s, Lu a , and Lu b antigens were typed using polyclonal antisera employing Indirect Antiglobulin Test. Antigens and phenotype frequencies were expressed as percentages. Results: From the 115 blood donor samples used for extended antigen typing in the Rh system, e antigen was found in 100% donors, followed by D [84.35%], C [81.74%], c [56.32%], and E [21.74%] with DCe/DCe (R 1 R 1 , 40.87% as the most common phenotype. k was found to be positive in 100% of donors and no K+k- phenotype was found in Kell system. For Kidd and Duffy blood group system, Jk(a+b+ and Fy(a-b- were the most common phenotypes with frequency of 52.17% and 48.69%, respectively. In the MNS system, 39.13% donors were typed as M+N+, 37.39% as M+N-, and 23.48% as M-N+. S+s+ was found in 24.35% of donors, S+s- in 8.69%, and S-s+ as the commonest amongst donors with 66.96%. No Lu(a+b+ or Lu(a+b- phenotypes were detected in 115 donors typed for Lutheran antigens. A rare Lu(a-b- phenotype was found in 2.61% donors. Conclusion: Data base for antigen frequency of various blood group systems in local donors help provide antigen negative compatible blood units to patients with multiple antibodies in order to formulate in-house red cells for antibody detection and identification and for preparing donor registry for rare blood groups.

  3. Blood group genotyping Genotipagem de grupos sangüíneos

    Directory of Open Access Journals (Sweden)

    Lilian Castilho

    2004-01-01

    Full Text Available Accurate phenotyping of red blood cells (RBCs can be difficult in transfusion-dependent patients such as those with thalassemia and sickle cells anemia because of the presence of previously transfused RBCs in the patient's circulation. Recently, the molecular basis associated with the expression of many blood group antigens was established. This allowed the development of a plethora of polymerase chain reaction-based tests for identification of the blood group antigens by testing DNA. The determination of blood group polymorphism at the genomic level facilitates the resolution of clinical problems that cannot be addressed by hemagglutination. They are useful to (a determine antigen types for which currently available antibodies are weakly reactive; (b type patients who have been recently transfused; (c identify fetuses at risk for hemolytic disease of the newborn; and (d to increase the reliability of repositories of antigen negative RBCs for transfusion. It is important to note that PCR based assays are prone to different types of errors that those observed with hemagglutination assays. For instance, contamination with amplified products may lead to false positive test results. In addition, the identification of a particular genotype does not necessarily mean that the antigen will be expressed on the RBC membrane.Os antígenos eritrocitários são herdados geneticamente e definidos por seqüências de aminoácidos específicos constituindo uma proteína ou por carboidratos ligados a estas proteínas ou à lipídios. A diversidade dos antígenos de grupos sangüíneos, como para qualquer outro traço biológico, encontra-se ao nível do gene. Existem atualmente mais de 250 antígenos eritrocitários que se encontram distribuídos em 29 sistemas de grupos sangüíneos, de acordo com a Nomenclatura da Sociedade Internacional de Transfusão Sangüínea (ISBT. Os genes que codificam 28 dos 29 sistemas de grupos sangüíneos já foram clonados e seq

  4. Blood groups and red cell acid phosphatase types in a Mixteca population resident in Mexico City.

    Science.gov (United States)

    Buentello, L.; García, P.; Lisker, R.; Salamanca, F.; Peñaloza, R.

    1999-01-01

    Several blood groups, ABO, Rh, Ss, Fy, Jk, and red cell acid phosphatase (ACP) types were studied in a native Mixteca population that has resided in Mexico City since 1950. Gene frequencies were obtained and used to establish admixture estimates with blacks and whites. The subjects came from three different geographical areas: High Mixteca, Low Mixteca, and Coast Mixteca. All frequencies were in Hardy-Weinberg equilibrium. The difference in the ABO frequencies was statistically significant when subjects from the three areas were compared simultaneously. Rh frequencies differed only between the High and the Low Mixteca populations. The ACP frequencies were similar between the Low Mixteca population and a previously reported Mestizo population. However, there were significant differences between the High Mixteca group and a Mestizo population, all the subjects being from Oaxaca. This is the first report of Ss, Fy, Jk, and ACP frequencies in a Mixteca population. Am. J. Hum. Biol. 11:525-529, 1999. Copyright 1999 Wiley-Liss, Inc.

  5. Role of histo-blood group antigens in primate enteric calicivirus infections.

    Science.gov (United States)

    Sestak, Karol

    2014-08-12

    Human noroviruses (NoV) are associated with large proportion of non-bacterial diarrhea outbreaks together with > 50% of food-associated diarrheas. The function of histo-blood group antigens (HBGAs) in pathogenesis of virus infection was implicated. Until recently however, due to lack of a robust animal and in vitro models of human NoV infection, only the partial knowledge concerning the virus pathogenesis (receptor, co-receptor and target cell) and absence of viable vaccine candidates were the frequently referenced attributes of this acute diarrheal illness. Recently, a novel group of enteric caliciviruses (CV) of rhesus macaque host origin was discovered and described. The new genus within the family Caliciviridae was identified: Rhesus Enteric CV, i.e., "Recovirus" (ReCV). ReCVs are genetically and biologically close relatives of human NoVs, exhibit similar genetic and biological features and are capable of being propagated in cell culture. ReCVs cause symptomatic disease (diarrhea and fever) in experimentally inoculated macaques. Formulation and evaluation of efficient NoV vaccine might take several years. As suggested by recent studies, inhibition of HBGAs or HBGA-based antivirals could meanwhile be exploited as vaccine alternatives. The purpose of this minireview is to provide the guidance in respect to newly available primate model of enteric CV infection and its similarities with human NoV in utilizing the HBGAs as potential virus co-receptors to indirectly address the unresolved questions of NoV pathogenesis and immunity. PMID:25392814

  6. Possible Correlation of Transfusion Transmitted Diseases with Rh type and ABO Blood Group System

    OpenAIRE

    Tyagi, Surabhi; Tyagi, Alok

    2013-01-01

    Background: Screening of blood is mandatory for transfusion transmitted diseases and is routinely done in the blood banks. As blood is the major source transmission of hepatitis B, hepatitis C, human immunodeficiency virus & many other diseases the hazards can be minimised by effective donor selection and screening.

  7. Prevalence of hepatitis B surface antigen & its subtypes in high risk group subjects & voluntary blood donors in Bombay.

    Science.gov (United States)

    Elavia, A J; Banker, D D

    1991-09-01

    HBsAg positive subjects belonging to high risk groups and voluntary blood donors were analysed for prevalence of HBsAg among various groups of subjects for ascertaining the carrier status among the voluntary blood donors, HBsAg subtype distribution, and association of HBsAg with blood groups and caste or religion. The prevalence of HBsAg varied from 2.02 per cent in voluntary blood donors to 58.38 per cent in patients of acute viral hepatitis. 70.5 per cent subjects had subtype 'ay' while 23.9 per cent of the subjects had subtype 'ad'. We also found compound 'ady' subtype in 5.6 per cent of our subjects. HBsAg/adr, a subtype not usually prevalent in India, was found in 30 of the 90 'ad' sera. Co-occurrence of HBsAg and anti-HBs was noted in 9 subjects. Homotypic anti-HBs was found to occur together mainly in voluntary blood donors, while heterotypic anti-HBs was found to occur together mainly multi-transfused patients. There was no significant correlation between HBsAg and blood group antigens and a relatively higher incidence of HBsAg among the Jain community was observed.

  8. Exploring Secondary Students' Epistemological Features Depending on the Evaluation Levels of the Group Model on Blood Circulation

    Science.gov (United States)

    Lee, Shinyoung; Kim, Heui-Baik

    2014-01-01

    The purpose of this study is to identify the epistemological features and model qualities depending on model evaluation levels and to explore the reasoning process behind high-level evaluation through small group interaction about blood circulation. Nine groups of three to four students in the eighth grade participated in the modeling practice.…

  9. Incompatibility boundaries for properties of community partitions

    CERN Document Server

    Browet, Arnaud; Sarlette, Alain

    2016-01-01

    We prove the incompatibility of certain desirable properties of community partition quality functions. Our results generalize the impossibility result of [Kleinberg 2003] by considering sets of weaker properties. In particular, we use an alternative notion to solve the central issue of the consistency property. (The latter means that modifying the graph in a way consistent with a partition should not have counterintuitive effects). Our results clearly show that community partition methods should not be expected to perfectly satisfy all ideally desired properties. We then proceed to show that this incompatibility no longer holds when slightly relaxed versions of the properties are considered, and we provide in fact examples of simple quality functions satisfying these relaxed properties. An experimental study of these quality functions shows a behavior comparable to established methods in some situations, but more debatable results in others. This suggests that defining a notion of good partition in communitie...

  10. Programmed cell death and hybrid incompatibility.

    Science.gov (United States)

    Frank, S A; Barr, C M

    2003-01-01

    We propose a new theory to explain developmental aberrations in plant hybrids. In our theory, hybrid incompatibilities arise from imbalances in the mechanisms that cause male sterility in hermaphroditic plants. Mitochondria often cause male sterility by killing the tapetal tissue that nurtures pollen mother cells. Recent evidence suggests that mitochondria destroy the tapetum by triggering standard pathways of programmed cell death. Some nuclear genotypes repress mitochondrial male sterility and restore pollen fertility. Normal regulation of tapetal development therefore arises from a delicate balance between the disruptive effects of mitochondria and the defensive countermeasures of the nuclear genes. In hybrids, incompatibilities between male-sterile mitochondria and nuclear restorers may frequently upset the regulatory control of programmed cell death, causing tapetal abnormalities and male sterility. We propose that hybrid misregulation of programmed cell death may also spill over into other tissues, explaining various developmental aberrations observed in hybrids.

  11. Sistema de grupo sangüíneo Duffy: biologia e prática transfusional Duffy blood group system: biology and transfusion practice

    Directory of Open Access Journals (Sweden)

    Eduardo Jens

    2005-06-01

    locus was mapped on chromosome 1q22-q23. The Fyª and Fy b antigens are encoded by FYA and FYB alleles, and are responsible for the Fy(a+b-, Fy(a-b+ and Fy(a+b+ phenotypes. They are carried by a 336 amino acid glycoprotein called DARC (Duffy Antigen/Receptor for Chemokines which has high affinity to chemokines, also being Plasmodium vivax receptors. The polymorphisms related to its alleles have led to the development of a PCR genotyping technique, which is useful for the safety of blood transfusion, and determining fetus-maternal incompatibilities. In the last decade, much research has been done to determine the biological role of blood group antigens. In this paper we reviewed the Duffy Blood Group System, especially in respect to transfusional practice and biological functions.

  12. 献血人群红细胞血型意外抗体的筛选%Screening unexpected antibody of red cells blood group in the blood donors

    Institute of Scientific and Technical Information of China (English)

    祝宏; 洪小珍; 吴亚玲; 励晓涛; 马开荣; 兰小飞; 朱发明

    2012-01-01

    目的:分析献血人群中红细胞血型意外抗体的情况,并对抗体特性进行确认.方法:应用O型红细胞结合PK7200血型检测系统筛选红细胞血型意外抗体,利用谱细胞鉴定抗体特性.结果:在献血人群中红细胞血型意外抗体阳性率为0.025%,意外抗体以抗M和冷凝集素为主.发现两例类孟买型血型和两例p血型.结论:献血人群中存在低比例的红细胞血型意外抗体,在献血者血型检测中应加以重视.%Objective;To determine unexpected antibody of red cells blood group in the blood donors and identify the characteristics of the antibody. Methods: The unexpected antibody was screened by 0 blood group cells and PK7200 system. Characteristics of the antibody were identified by panel cells.. Results: The prevalence rate of unexpected antibody was 0.025% in the blood donors. The common unexpected antibodies were anti - M and cold ag-glutinin. Two cases with para - Bombay phenotype and two cases with p phenotype were found. Conclusion; Low prevalence of unexpected antibody existed in the blood donors. It is important to screen unexpected antibody in donor's blood grouping.

  13. Analysis of ABO blood group subtype identification and serology%ABO血型亚型检测与血清学

    Institute of Scientific and Technical Information of China (English)

    何毅勇

    2015-01-01

    目的:通过对ABO血型亚型的检测和血清学分析,探讨ABO亚型的输血安全。方法选取本院输血科43例患者的正反型不符血液标本,检测红细胞ABH抗原、ABO血型系统抗体;并用ABO正反定型、唾液血型物质测定、吸收释放试验等进行血清学检查。结果43例标本共检出ABO血型亚型13种,其中A亚型8例(18.60%),B亚型32例(78.05%),类孟买型1例,cisAB型2例(4.65%)。结论对于ABO血型亚型的鉴定应采用多种血清学方法进行检测,为临床输血提供安全保障。%ObjectiveTo investigate the transfusion safety of ABO blood group subtype through ABO blood group subtype identification and serology analysis.Methods 43 patients whose positive and negative type did not match the blood specimen were chosen.Their ABH antigen of red blood cells and antibody of ABO blood group system were detected.Their serology was checked with application of ABO positive and negative stereotypes,determination of blood group substances in saliva,absorption and release test and so on.ResultsIn these 43 cases,13 cases were diagnosed with ABO blood group subtype,among which 8 were A subtype(18.60%), 32 were B subtype(78.05%),1 was Para-Bombay type and 2 were cisAB type(4.65%). Conclusion Blood group identification of ABO blood group subtype should be checked by a variety of serological methods,which guarantees the safety of clinical blood transfusion.

  14. Adenoid and tonsil surgeries in children: How relevant is pre-operative blood grouping and cross-matching?

    OpenAIRE

    Lucky Onotai; Opubo da Lilly-Tariah

    2013-01-01

    Background: As a part of pre-operative evaluation, several otolaryngologists group and cross-match blood routinely for children undergoing adenoid and tonsil surgeries. This practice has generated several debates either in support or against this practice. The aim of this study is to critically evaluate the incidence of post-tonsillectomy (with or without adenoidectomy) bleeding and blood transfusions in otherwise healthy children with adenoid/tonsil pathologies conducted in the University of...

  15. Cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) mutations associated with the domestic cat AB blood group

    OpenAIRE

    Millon Lee V; Pedersen Niels C; Grahn Robert A; Niini Tirri; Bighignoli Barbara; Polli Michele; Longeri Maria; Lyons Leslie A

    2007-01-01

    Abstract Background The cat has one common blood group with two major serotypes, blood type A that is dominant to type B. A rare type AB may also be allelic and is suspected to be recessive to A and dominant to B. Cat blood type antigens are defined, N-glycolylneuraminic acid (NeuGc) is associated with type A and N-acetylneuraminic acid (NeuAc) with type B. The enzyme cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) determines the sugar bound to the red cell by converting NeuAc...

  16. First Report of Three Cases of a Rare Blood Group Oh “Bombay Phenotype” in a Family in Yazd, Iran

    Directory of Open Access Journals (Sweden)

    H Javadzadeh Shahshahani

    2013-03-01

    Full Text Available The Bombay (Oh Phenotype is a rare blood group. Phenotypes of this group lack H antigens on their red blood cell membrane and have strongly reactive anti-H in the serum for which patients can receive only autologus or Bombay phenotype red blood cells. We report three cases with Bombay blood group in the city of Yazd to emphasize the transfusion challenges in such patients.

  17. Molecular cloning and protein structure of a human blood group Rh polypeptide

    International Nuclear Information System (INIS)

    cDNA clones encoding a human blood group Rh polypeptide were isolated from a human bone marrow cDNA library by using a polymerase chain reaction-amplified DNA fragment encoding the known common N-terminal region of the Rh proteins. The entire primary structure of the Rh polypeptide has been deduced from the nucleotide sequence of a 1384-base-pair-long cDNA clone. Translation of the open reading frame indicates that the Rh protein is composed of 417 amino acids, including the initiator methionine, which is removed in the mature protein, lacks a cleavable N-terminal sequence, and has no consensus site for potential N-glycosylation. The predicted molecular mass of the protein is 45,500, while that estimated for the Rh protein analyzed in NaDodSO4/polyacrylamide gels is in the range of 30,000-32,000. These findings suggest either that the hydrophobic Rh protein behaves abnormally on NaDodSO4 gels or that the Rh mRNA may encode a precursor protein, which is further matured by a proteolytic cleavage of the C-terminal region of the polypeptide. Hydropathy analysis and secondary structure predictions suggest the presence of 13 membrane-spanning domains, indicating that the Rh polypeptide is highly hydrophobic and deeply buried within the phospholipid bilayer. These results suggest that the expression of the Rh gene(s) might be restricted to tissues or cell lines expressing erythroid characters

  18. Gene arrangement at the Rhesus blood group locus of chimpanzees detected by fiber-FISH.

    Science.gov (United States)

    Suto, Y; Ishikawa, Y; Hyodo, H; Ishida, T; Kasai, F; Tanoue, T; Hayasaka, I; Uchikawa, M; Juji, T; Hirai, M

    2003-01-01

    The Rhesus (Rh) blood group system in humans is encoded by two genes with high sequence homology. These two genes, namely, RHCE and RHD, have been implied to be duplicated during evolution. However, the genomic organization of Rh genes in chimpanzees and other nonhuman primates has not been precisely studied. We analyzed the arrangement of the Rh genes of chimpanzees (Pan troglodytes) by two-color fluorescence in situ hybridization on chromatin DNA fibers (fiber-FISH) using two genomic DNA probes that respectively contain introns 3 and 7 of human RH genes. Among the five chimpanzees studied, three were found to be homozygous for the two-Rh-gene type, in an arrangement of Rh (5'-->3') - Rh (3'chimpanzees was about 50 kb longer than that in humans. The remaining two chimpanzees were homozygous for a four-Rh-gene type, in an arrangement of Rh (5'-->3') - Rh (3'interspecific genomic variations in the Rh gene locus in Hominoids would shed further light on reconstructing the genomic pathways of Rh gene duplication during evolution. PMID:14610358

  19. Blood-group-related carbohydrates are expressed in organotypic cultures of human skin and oral mucosa

    DEFF Research Database (Denmark)

    Grøn, B; Andersson, A; Dabelsteen, Erik

    1999-01-01

    Cellular maturation and migration are usually associated with changes in cell-surface carbohydrates, but the relationship between these changes and cell behaviour is at present largely unknown. To investigate whether an organotypic culture system can be used as an in vitro model to study the func......Cellular maturation and migration are usually associated with changes in cell-surface carbohydrates, but the relationship between these changes and cell behaviour is at present largely unknown. To investigate whether an organotypic culture system can be used as an in vitro model to study...... the function of cell-surface carbohydrates, we established organotypic cultures of skin and buccal mucosa. In these cultures, keratinocytes are grown at the air-liquid interface on a supporting matrix consisting of homologous fibroblasts embedded in a collagen type I gel. We examined the expression of blood......-group-related carbohydrate structures, including Lewis x, sialylated Lewis x, Lewis y, Lewis a, and Lewis b, on the surface of epithelial cells in the cultures. We compared the results with the expression of more well-established markers, including cytokeratins, integrins, bullous pemphigoid antigen and laminin, in the same...

  20. Possible genetical pathways for the biosynthesis of blood group mucopolysaccharides. Vox Sang 1959:4:97-119.

    Science.gov (United States)

    Watkins, W M; Morgan, W T

    1993-01-01

    This paper put forward possible biosynthetic pathways for the formation of the blood group A, B, H and Lewis antigens based on the limited knowledge of their chemistry and genetics that was available in 1959. The schemes proposed that genes at four independent loci ABO, HH, Lele and Sese interacted to give the five specificities A, B, H, Lea and Leb found in secretions and that the primary products of the blood group genes were not the antigens but enzymes that catalysed the sequential addition of single sugars to complete the determinants. PMID:7685971

  1. Atomic force microscopy measurements reveal multiple bonds between Helicobacter pylori blood group antigen binding adhesin and Lewis b ligand.

    Science.gov (United States)

    Parreira, P; Shi, Q; Magalhaes, A; Reis, C A; Bugaytsova, J; Borén, T; Leckband, D; Martins, M C L

    2014-12-01

    The strength of binding between the Helicobacter pylori blood group antigen-binding adhesin (BabA) and its cognate glycan receptor, the Lewis b blood group antigen (Le(b)), was measured by means of atomic force microscopy. High-resolution measurements of rupture forces between single receptor-ligand pairs were performed between the purified BabA and immobilized Le(b) structures on self-assembled monolayers. Dynamic force spectroscopy revealed two similar but statistically different bond populations. These findings suggest that the BabA may form different adhesive attachments to the gastric mucosa in ways that enhance the efficiency and stability of bacterial adhesion.

  2. Protoplasmic Incompatibility in PODOSPORA ANSERINA: a Possible Function for Incompatibility Genes.

    Science.gov (United States)

    Boucherie, H; Bernet, J

    1980-10-01

    The suppression of protoplasmic incompatibility resulting from nonallelic gene interactions has been obtained by the coupled effect of mutations in the modA and modB genes (Bernet 1971). Due to their female sterility, modA modB strains provide an experimental tool to determine whether or not the mod and incompatibility loci are involved in a function other than protoplasmic incompatibility. Present results show that modA modB female sterility is a nonautonomous trait since heterokaryotic mycelia that include a modA modB nucleus and a female fertile nucleus (wild-type, modA or modB) produce modA modB protoperithecia, which are also formed by culture on medium supplemented with specific amino acids. Using modA modB strains, which are sterile at 32 degrees and fertile at 26 degrees , we have shown that the mod genes have no specific sequential timing. Indeed, the mod mutations may prevent the achievement of the female sexual cycle at any developmental stage from before early differentiation of protoperithecia until ascospore maturation. Employing different modA and modB mutations, we have shown that protoperithecia in modA modB cultures are generally distributed in female fertile rings; this result indicates that protoperithecia occur only in mycelial areas that have a restricted range of age at the time that modA modB thalli complete growth. Furthermore, nonsense mutations of incompatibility genes suppress the modA modB female fertile rings or restrict their width, suggesting that incompatibility loci, like the mod loci, are involved in protoperithecium formation. Taken together, these results lead to the postulate that mod and incompatibility genes do not determine, sensu stricto, protoperithecial function, as previously supposed (Boucherie and Bernet 1974), but may be involved in the homeostatic control of stationary cell functions essential for the complete development of the female sexual cycle.

  3. Perioperative management of patient with Bombay blood group undergoing mitral valve replacement

    Directory of Open Access Journals (Sweden)

    Shio Priye

    2015-01-01

    Full Text Available Bombay red blood cell phenotype is an extremely rare blood type for which patients can receive only autologous or Bombay phenotype red blood cells. We report a case of stenotic mitral valve with Bombay phenotype who underwent minimal invasive right lateral thoracotomy for the replacement of the mitral valve. A male patient from Bangladesh presented to the hospital with New York Heart Association III symptoms. His medical evaluation revealed severe mitral valve stenosis and mild aortic valve regurgitation. The patient received erythropoietin, intravenous iron succinate and folic acid tablets. Autologous blood transfusion was carried out. The mitral valve was replaced with a prosthetic valve successfully. After weaning off from cardiopulmonary bypass, heparinisation was corrected with protamine. Post-operatively, the patient received autologous red blood cells. The patient recovered after 1-day of inotropic support with adrenaline and milrinone, and diuretics and was discharged on the 5 th post-operative day.

  4. Perioperative management of patient with Bombay blood group undergoing mitral valve replacement.

    Science.gov (United States)

    Priye, Shio; Sathyanarayan, J; Shivaprakash, S; Reddy, Durgaprasad

    2015-12-01

    Bombay red blood cell phenotype is an extremely rare blood type for which patients can receive only autologous or Bombay phenotype red blood cells. We report a case of stenotic mitral valve with Bombay phenotype who underwent minimal invasive right lateral thoracotomy for the replacement of the mitral valve. A male patient from Bangladesh presented to the hospital with New York Heart Association III symptoms. His medical evaluation revealed severe mitral valve stenosis and mild aortic valve regurgitation. The patient received erythropoietin, intravenous iron succinate and folic acid tablets. Autologous blood transfusion was carried out. The mitral valve was replaced with a prosthetic valve successfully. After weaning off from cardiopulmonary bypass, heparinisation was corrected with protamine. Post-operatively, the patient received autologous red blood cells. The patient recovered after 1-day of inotropic support with adrenaline and milrinone, and diuretics and was discharged on the 5(th) post-operative day.

  5. Proteomics analysis of compatibility and incompatibility in grafted cucumber seedlings.

    Science.gov (United States)

    Xu, Qing; Guo, Shi-Rong; Li, Lin; An, Ya-Hong; Shu, Sheng; Sun, Jin

    2016-08-01

    Graft compatibility between rootstock and scion is the most important factor influencing the survival of grafted plants. In this study, we used two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS) to investigate differences in leaf proteomes of graft-compatible and graft-incompatible cucumber (Cucumis sativus L.)/pumpkin (Cucurbita L.) combinations. Cucumber seedlings were used as the scions and two pumpkin cultivars with strongly contrasting grafting compatibilities were used as the rootstocks. Non-grafted and self-grafted cucumber seedlings served as control groups. An average of approximately 500 detectable spots were observed on each 2-DE gel. A total of 50 proteins were differentially expressed in response to self-grafting, compatible-rootstock grafting, and incompatible-rootstock grafting and were all successfully identified by MALDI-TOF/TOF MS. The regulation of Calvin cycle, photosynthetic apparatus, glycolytic pathway, energy metabolism, protein biosynthesis and degradation, and reactive oxygen metabolism will probably contribute to intensify the biomass and photosynthetic capacity in graft-compatible combinations. The improved physiological and growth characteristics of compatible-rootstock grafting plants are the result of the higher expressions of proteins involved in photosynthesis, carbohydrate and energy metabolism, and protein metabolism. At the same time, the compatible-rootstock grafting regulation of stress defense, amino acid metabolism, and other metabolic functions also plays important roles in improvement of plant growth. PMID:27070289

  6. Blood group antigen studies using CdTe quantum dots and flow cytometry.

    Science.gov (United States)

    Cabral Filho, Paulo E; Pereira, Maria I A; Fernandes, Heloise P; de Thomaz, Andre A; Cesar, Carlos L; Santos, Beate S; Barjas-Castro, Maria L; Fontes, Adriana

    2015-01-01

    New methods of analysis involving semiconductor nanocrystals (quantum dots [QDs]) as fluorescent probes have been highlighted in life science. QDs present some advantages when compared to organic dyes, such as size-tunable emission spectra, broad absorption bands, and principally exceptional resistance to photobleaching. Methods applying QDs can be simple, not laborious, and can present high sensibility, allowing biomolecule identification and quantification with high specificity. In this context, the aim of this work was to apply dual-color CdTe QDs to quantify red blood cell (RBC) antigen expression on cell surface by flow cytometric analysis. QDs were conjugated to anti-A or anti-B monoclonal antibodies, as well as to the anti-H (Ulex europaeus I) lectin, to investigate RBCs of A1, B, A1B, O, A2, and Aweak donors. Bioconjugates were capable of distinguishing the different expressions of RBC antigens, both by labeling efficiency and by flow cytometry histogram profile. Furthermore, results showed that RBCs from Aweak donors present fewer amounts of A antigens and higher amounts of H, when compared to A1 RBCs. In the A group, the amount of A antigens decreased as A1 > A3 > AX = Ael, while H antigens were AX = Ael > A1. Bioconjugates presented stability and remained active for at least 6 months. In conclusion, this methodology with high sensibility and specificity can be applied to study a variety of RBC antigens, and, as a quantitative tool, can help in achieving a better comprehension of the antigen expression patterns on RBC membranes. PMID:26185442

  7. Russia and Human Rights: Incompatible Opposites?

    Directory of Open Access Journals (Sweden)

    Bill Bowring

    2009-04-01

    Full Text Available The Article raises in his article “Russia and human rights: incompatible opposites?” the question, if the currently complicated relations between Russia and the CoE concerning Russia’s obligations under the ECHR are at breaking-point. In regard to this issue he gives a description of the history of law in Russia to prove the pre-existing tradition of argument about human rights.

  8. The Construal (In)compatibility Effect

    DEFF Research Database (Denmark)

    Yang, Xiaojing; Ringberg, Torsten; Mao, Huifang;

    2011-01-01

    This research examines how consumers with a creative mind-set are persuaded by advertising claims construed at different levels (i.e., abstract vs. concrete ad claims). Across four experiments, we show that consumers with a creative mindset are more persuaded by ad claims construed at a level...... incompatible with their mental construal, while ad claims construed at a level compatible with consumers' mental construal are more effective for those who possess a less creative mindset. We document that such differences in persuasion are driven by the fact that consumers with a creative (less creative) mind-set...

  9. Delayed hyperacute rejection in a patient who developed clostridium difficile infection after ABO-incompatible kidney transplantation

    Directory of Open Access Journals (Sweden)

    Gerald S Lipshutz

    2010-11-01

    Full Text Available Gerald S Lipshutz1, Elaine F Reed2, Phuong-Chi Pham3, Jeffrey M Miller4, Jennifer S Singer5, Gabriel M Danovitch6, Alan H Wilkinson6, Dean W Wallace7, Suzanne McGuire6, Phuong-Truc Pham8, Phuong-Thu Pham61Department of Surgery, Kidney and Pancreas Transplant Program, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; 2Department of Pathology and Laboratory Medicine-Immunogenetics, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; 3Department of Medicine, Nephrology Division, UCLA-Olive View Medical Center, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; 4Department of Medicine, Hematology Oncology Division, UCLA-Olive View Medical Center, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; 5Department of Surgery and Urology, Kidney and Pancreas Transplant Program, 6Department of Medicine, Nephrology Division, Kidney and Pancreas Transplant Program, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; 7Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; 8Department of Science, Penn State University, Worthington-Scranton, Dunmore, PA, USAAbstract: Over the past decade ABO incompatible transplantation has emerged as an important potential source for increasing living kidney transplantation in selected transplant centers. Early reports suggest that patients who have elevated serum anti-blood group antibody titers (anti-A/B before transplantation and a rebound antibody production after antibody removal may be at high immunological risk. With currently available immune modulation protocols and immunosuppressive therapy, excellent short- and long-term patient and graft survival rates have been achieved even in those with high anti-A/B antibody titers before plasmapheresis or immunoadsorption. Nonetheless, acute infection with an organism possessing surface markers analogous to blood group antigens such as carbohydrate structures on

  10. Blood Basics

    Science.gov (United States)

    ... Patient Group Links Advocacy Toolkit Home For Patients Blood Basics Blood is a specialized body fluid. It ... about 9 pints. Jump To: The Components of Blood and Their Importance Many people have undergone blood ...

  11. Effects of group size and gentling on behaviour, selected organ masses and blood constituents in female Rivm : TOX rats

    NARCIS (Netherlands)

    van Bergeijk, J P; van Herck, H; de Boer, S.F.; Meijer, G W; Hesp, A P; van der Gugten, J; Beynen, A C

    1990-01-01

    The effects of group size (individually versus 3 in a cage) and gentling on behaviour and blood constituents were studied in female rats. Gentled rats showed less freezing and/or escaping when approached in an objective handling test than non-gentled rats; the type of caging had no significant influ

  12. Thrombotic thrombocytopenic purpura after allogeneic stem cell transplantation : a survey of the European Group for Blood and Marrow Transplantation (EBMT)

    NARCIS (Netherlands)

    Ruutu, T; Hermans, J; Niederwieser, D; Gratwohl, A; Kiehl, M; Volin, L; Bertz, H; Ljungman, P; Spence, D; Verdonck, LF; Prentice, HG; Bosi, A; du Toit, CE; Brinch, L; Apperley, JF

    2002-01-01

    A survey was carried out among the European Group for Blood and Marrow Transplantation (EBMT) centres to determine the incidence, risk factors, treatment and outcome of thrombotic thrombocytopenic purpura (TTP) following allogeneic haematopoietic stem cell transplantation. TTP was defined as the sim

  13. Mechanisms of self-incompatibility and unilateral incompatibility in diploid potato (Solanum tuberosum L.).

    NARCIS (Netherlands)

    Eijlander, R.

    1998-01-01

    In chapter 1 an overview is given of the major mechanisms operating in Angiosperms that prevent or limit the degree of inbreeding. The two major systems that function on the basis of interaction between pollen and stigma/style, are the sporophytic and the gametophytic self-incompatibility systems (S

  14. Ending tolerance as a solution to incompatibility: The Danish ‘crisis of multiculturalism’

    DEFF Research Database (Denmark)

    Hervik, Peter

    2012-01-01

    group discussion in Denmark on the integration of Muslims into Danish society. This idea of incompatibility between native Danes and Muslim ‘newcomers’ has become a salient feature of what is termed ‘value-based journalism’ and ‘value-based politics’ in the last decade. This article traces the origin...

  15. Different sensitivity of rituximab-treatment to B-cells between ABO-incompatible kidney and liver transplantation.

    Science.gov (United States)

    Morimoto, Hiroshi; Ide, Kentaro; Tanaka, Yuka; Ishiyama, Kohei; Ohira, Masahiro; Tahara, Hiroyuki; Akita, Tomonori; Tanaka, Junko; Ohdan, Hideki

    2016-06-01

    A desensitization protocol with rituximab is currently widely used for kidney transplantation (KT) and liver transplantation (LT) across the ABO blood group-incompatible (ABO-I) barrier. However, it remains to be elucidated whether rituximab is equally effective for B-cell and T-cell immune responses in both KT and LT recipients. To clarify these effects of rituximab, we enrolled 46 KT and 77 LT recipients in this study. The proportion of peripheral blood B-cells was determined at the perioperative period. T-cell responses to allostimulation were evaluated by a mixed lymphocyte reaction (MLR) assay. One week after rituximab administration, peripheral B-cells became undetectable in ABO-I KT recipients but remained detectable in some of the ABO-I LT recipients; B-cells were undetectable in both groups by week 2. B-cells remained below the detection limit throughout the first year in the ABO-I KT recipients, whereas they reappeared in the periphery after 6months in the ABO-I LT recipients. There were no significant differences in alloreactive T-cell responses based on MLR analyses between ABO-I and ABO-compatible groups. This study indicates that rituximab has differing B-cell sensitivity between KT and LT recipients and a minimal effect on the alloreactive T-cell responses in KT and LT recipients. PMID:27085793

  16. Blood group antigen studies using CdTe quantum dots and flow cytometry

    Directory of Open Access Journals (Sweden)

    Cabral Filho PE

    2015-07-01

    Full Text Available Paulo E Cabral Filho,1 Maria IA Pereira,1 Heloise P Fernandes,2 Andre A de Thomaz,3 Carlos L Cesar,3 Beate S Santos,4 Maria L Barjas-Castro,2 Adriana Fontes1 1Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco, 2Centro de Hematologia e Hemoterapia, Universidade Estadual de Campinas, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, 3Departamento de Eletrônica Quântica, Instituto de Física Gleb Wataghin, Universidade Estadual de Campinas, Campinas, São Paulo, 4Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Recife, PE, Brazil Abstract: New methods of analysis involving semiconductor nanocrystals (quantum dots [QDs] as fluorescent probes have been highlighted in life science. QDs present some advantages when compared to organic dyes, such as size-tunable emission spectra, broad absorption bands, and principally exceptional resistance to photobleaching. Methods applying QDs can be simple, not laborious, and can present high sensibility, allowing biomolecule identification and quantification with high specificity. In this context, the aim of this work was to apply dual-color CdTe QDs to quantify red blood cell (RBC antigen expression on cell surface by flow cytometric analysis. QDs were conjugated to anti-A or anti-B monoclonal antibodies, as well as to the anti-H (Ulex europaeus I lectin, to investigate RBCs of A1, B, A1B, O, A2, and Aweak donors. Bioconjugates were capable of distinguishing the different expressions of RBC antigens, both by labeling efficiency and by flow cytometry histogram profile. Furthermore, results showed that RBCs from Aweak donors present fewer amounts of A antigens and higher amounts of H, when compared to A1 RBCs. In the A group, the amount of A antigens decreased as A1 > A3 > AX = Ael, while H antigens were AX = Ael > A1. Bioconjugates presented stability and remained active for at least 6 months. In conclusion

  17. Complement Interception Across Humoral Incompatibility in Solid Organ Transplantation: A Clinical Perspective.

    Science.gov (United States)

    Biglarnia, Ali-Reza; Ekdahl, Kristina N; Nilsson, Bo

    2015-01-01

    The humoral barrier in transplant biology is the result of preformed donor-specific antibodies (DSAs), directed either against human leukocyte antigens (HLA) or non-HLA antigens such as blood group (ABO) molecules. The term "sensitization" applies to patients carrying these antibodies. Transplantation is widely accepted as a life-saving opportunity for patients with terminal end-organ disease. However, in sensitized patients, transplant outcome is hampered by antibody-mediated rejection (AMR) as a consequence of DSA exposure. Furthermore, sensitized patients have limited access to "matched" organs from the both living and deceased donor pool.Considering the crucial role of the complement system in the pathophysiology of AMR and the availability of complement intervention therapeutics, there is a growing interest in complement-targeting strategies. This review highlights the emerging importance of monitoring and modulation of the complement system in the context of enabling transplantation across humoral incompatibility in sensitized recipients with preformed anti-HLA or natural anti-ABO antibodies. It also discusses the significance of the complement system in the induction of accommodation and further emphasizes current and future perspectives of novel complement therapeutics.

  18. Functional groups grafted nonwoven fabrics for blood filtration-The effects of functional groups and wettability on the adhesion of leukocyte and platelet

    Energy Technology Data Exchange (ETDEWEB)

    Yang Chao [State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240 (China); Cao Ye [Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu 610081 (China); Sun Kang, E-mail: ksun@sjtu.edu.cn [State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240 (China); Liu Jiaxin; Wang Hong [Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu 610081 (China)

    2011-01-15

    In this work, the effects of grafted functional groups and surface wettability on the adhesion of leukocyte and platelet were investigated by the method of blood filtration. The filter materials, poly(butylene terephthalate) nonwoven fabrics bearing different functional groups including hydroxyl (OH), carboxyl (COOH), sulfonic acid group (SO{sub 3}H) and zwitterionic sulfobetaine group ({sup +}N((CH{sub 3}){sub 2})(CH{sub 2}){sub 3}SO{sub 3}{sup Circled-Minus }) with controllable wettability were prepared by UV radiation grafting vinyl monomers with these functional groups. Our results emphasized that both surface functional groups and surface wettability had significant effects on the adhesion of leukocyte and platelet. In the case of filter materials with the same wettability, leukocytes adhering to filter materials decreased in the order: the surface bearing OH only > the surface bearing both OH and COOH > the surface bearing sulfobetaine group > the surface bearing SO{sub 3}H, while platelets adhering to filter materials decreased as the following order: the surface bearing SO{sub 3}H > the surface bearing both OH and COOH > the surface bearing OH only > the surface bearing sulfobetaine group. As the wettability of filter materials increased, both leukocyte and platelet adhesion to filter materials declined, except that leukocyte adhesion to the surface bearing OH only remained unchanged.

  19. Higher frequency of secretor phenotype in O blood group – its benefits in prevention and/or treatment of some diseases

    Directory of Open Access Journals (Sweden)

    Mohamad Salih Jaff

    2010-11-01

    Full Text Available Mohamad Salih JaffPathology Department, Hawler Medical University (Formerly Salahaddin University, Erbil, Kurdistan Region, IraqAbstract: ABO blood groups and secretor status are important in clinical and forensic medicine and in relation to some diseases. There are geographic and racial differences in their frequencies, but the frequency of secretor status in different ABO blood group systems has not been determined yet. Therefore, the aim of this study was mainly to determine this point. Blood and saliva from 762 randomly selected apparently healthy adult individuals (480 men and 282 women were examined to determine their ABO and Rhesus blood groups by standard conventional methods, and their secretor status by using Lewis blood grouping and/or hemagglutination inhibition test of saliva. Results showed that 76.1% of the study population were ABH blood group antigens secretors and 23.9% were nonsecretors. The frequencies of secretor status in different ABO blood groups were 70.1% in group A, 67.8% in group B, 67.9% in group AB, and 88.3% in group O. In conclusion, blood group O individuals have significantly higher frequency of secretor status than non-O blood group individuals. This finding would be beneficial to them, protecting them, at least partially, from certain malignancies or allowing them to have less aggressive disease, and this finding might be useful in enhancing further studies and research in this direction.Keywords: blood group O, ABO blood groups, secretor phenotype, frequency, malignancies, prevention and/or treatment

  20. Novel UDP-GalNAc Derivative Structures Provide Insight into the Donor Specificity of Human Blood Group Glycosyltransferase.

    Science.gov (United States)

    Wagner, Gerd K; Pesnot, Thomas; Palcic, Monica M; Jørgensen, Rene

    2015-12-25

    Two closely related glycosyltransferases are responsible for the final step of the biosynthesis of ABO(H) human blood group A and B antigens. The two enzymes differ by only four amino acid residues, which determine whether the enzymes transfer GalNAc from UDP-GalNAc or Gal from UDP-Gal to the H-antigen acceptor. The enzymes belong to the class of GT-A folded enzymes, grouped as GT6 in the CAZy database, and are characterized by a single domain with a metal dependent retaining reaction mechanism. However, the exact role of the four amino acid residues in the specificity of the enzymes is still unresolved. In this study, we report the first structural information of a dual specificity cis-AB blood group glycosyltransferase in complex with a synthetic UDP-GalNAc derivative. Interestingly, the GalNAc moiety adopts an unusual yet catalytically productive conformation in the binding pocket, which is different from the "tucked under" conformation previously observed for the UDP-Gal donor. In addition, we show that this UDP-GalNAc derivative in complex with the H-antigen acceptor provokes the same unusual binding pocket closure as seen for the corresponding UDP-Gal derivative. Despite this, the two derivatives show vastly different kinetic properties. Our results provide a important structural insight into the donor substrate specificity and utilization in blood group biosynthesis, which can very likely be exploited for the development of new glycosyltransferase inhibitors and probes.

  1. Production of soluble recombinant proteins with Kell, Duffy and Lutheran blood group antigen activity, and their use in screening human sera for Kell, Duffy and Lutheran antibodies.

    Science.gov (United States)

    Ridgwell, K; Dixey, J; Scott, M L

    2007-10-01

    The aim of this study was to show that soluble recombinant (sr) proteins can mimic blood group antigens and be used to screen human sera for blood-group-specific antibodies. The blood of all pregnant women and pretransfusion patients should be screened for blood-group-specific antibodies to identify and monitor pregnancies at risk of haemolytic disease of the foetus and newborn (HDFN), and to prevent haemolytic transfusion reactions. Current antibody screening and identification methods use human red blood cell panels, which can complicate antibody identification if more than one antibody specificity is present. COS-7 cells were transfected to produce sr forms of the extracellular domains of the red blood cell membrane proteins that express Kell, Duffy or Lutheran blood group antigens. These sr proteins were used to screen for and identify anti-Kell, anti-Duffy or anti-Lutheran blood-group-specific allo-antibodies in human sera by haemagglutination inhibition and in solid-phase enzyme-linked immunosorbent assays (ELISAs). There is a positive correlation (correlation coefficient 0.605, P value 0.002) between antibody titre by standard indirect antiglobulin test (IAT) and signal intensity in the ELISA test. This work shows that sr proteins can mimic blood group antigens and react with human allogeneic antibodies, and that such proteins could be used to develop solid-phase, high-throughput blood group antibody screening and identification platforms. PMID:17725551

  2. Extensive adaptive changes occur in the transcriptome of Streptococcus agalactiae (group B streptococcus in response to incubation with human blood.

    Directory of Open Access Journals (Sweden)

    Laurent Mereghetti

    Full Text Available To enhance understanding of how Streptococcus agalactiae (group B streptococcus, GBS adapts during invasive infection, we performed a whole-genome transcriptome analysis after incubation with whole human blood. Global changes occurred in the GBS transcriptome rapidly in response to blood contact following shift from growth in a rich laboratory medium. Most (83% of the significantly altered transcripts were down-regulated after 30 minutes of incubation in blood, and all functional categories of genes were abundantly represented. We observed complex dynamic changes in the expression of transcriptional regulators and stress response genes that allow GBS to rapidly adapt to blood. The transcripts of relatively few proven virulence genes were up-regulated during the first 90 minutes. However, a key discovery was that genes encoding proteins involved in interaction with the host coagulation/fibrinolysis system and bacterial-host interactions were rapidly up-regulated. Extensive transcript changes also occurred for genes involved in carbohydrate metabolism, including multi-functional proteins and regulators putatively involved in pathogenesis. Finally, we discovered that an incubation temperature closer to that occurring in patients with severe infection and high fever (40 degrees C induced additional differences in the GBS transcriptome relative to normal body temperature (37 degrees C. Taken together, the data provide extensive new information about transcriptional adaptation of GBS exposed to human blood, a crucial step during GBS pathogenesis in invasive diseases, and identify many new leads for molecular pathogenesis research.

  3. Plasmodial incompatibility in the myxomycetes: a review

    Directory of Open Access Journals (Sweden)

    Haskins EF

    2012-03-01

    Full Text Available Two myxomycete phaneroplasmodia of the same species undergo somatic fusion only if they are phenotypically identical for a complex genetic incompatibility system. This system consists of a three tiered polygenic complex with dominant and recessive alleles. Thus, plasmodia must be phenotypically identical for approximately 16 loci in order to fuse (CC and Cc are phenotypically identical, but different from cc. The first level of the system (having a minimum of seven Fus loci controls membrane fusion, and it apparently prevents fusion unless the two plasmodia have identical membrane or slime sheath components. The second level (having a minimum of six Cz loci produces a rapid lysis of a small mixed region, of the two plasmodia, if membrane fusion has occurred. This lysis is directional in that it targets the recessive phenotype, and it is apparently triggered by some pre-formed substances when they come into contact with a different plasmodium. The third level (having a minimum of three Let loci comes into play if membrane fusion occurs and there is no rapid lysis of the mixed plasmodium. It produces a slow lethal reaction, which targets and degrades the nuclei of the recessive phenotype. This reaction occurs over a period of five to twenty hours and requires the synthesis of new RNA and proteins. Since, this complex system produces a minimum of 65,536 different incompatibility phenotypes, it is highly unlikely that any two phaneroplasmodia will undergo a successful fusion unless they are very closely related. Species with aphaneroplasmoida apparently have a similar system, but species with small protoplasmodia do not appear to undergo any type of plasmodial fusion.

  4. Role of blood grouping as a prognostic marker in breast carcinoma its relationship with histological and hormonal prognostic markers

    Directory of Open Access Journals (Sweden)

    Lokesh Haswani

    2014-01-01

    Full Text Available Context: Breast carcinomas are one of the leading causes of mortality and morbidity in our country. Estrogen receptor (ER and progesterone receptor (PR status plays a very important role in therapeutic decisions in managing these patients. ABO and Rh blood type has been associated with risk and survival for several malignancies. Aims and Objectives: To know the frequency of ER and PR positivity status in the semi-urban population. To relate ABO/Rh blood group, ER and PR status with histopathological stage and Nottingham prognostic index (NPI. Materials and Methods: This was a retrospective study carried out on 45 cases from July 2012 to December 2013 who underwent mastectomy for breast cancer were included in our study. Histopathological grade of the tumor, lymph node invasion was noted. NPI was calculated. Immunohistochemistry was done using antibodies against ER and PR. Blood grouping and Rh typing was done. Descriptive statistics and Chi-square tests were done using SPSS package 20. P < 0.05 was considered to be significant. Results: In our study, maximum number of cases were in the fourth decade of life with a mean age of 52 years. ER and PRs were positive in 23/45 (51.1% of cases. Most of the ER and PR negative patients were in the premenopausal group. Lymph node-positive tumors were ER negative (54% and PR negative (58%. Patients in our study belonged to Group B (35.5% and Group O (35.5%. Eighty percent of Rh negative cases were ER and PR positive. A 2 × 2 table correlating ER and PR positivity with Rh negative status revealed a positive correlation with P < 0.05. Majority of ER and PR negative tumors belonged to Groups B and O. Conclusion: Majority of the patients were in premenopausal age group with 51.1% of our cases were ER and PR positive. Majority of Rh negative17 patients were ER and PR positive.

  5. In vitro evaluation of the role of the Duffy blood group in erythrocyte invasion by Plasmodium vivax

    OpenAIRE

    1989-01-01

    A short-term in vitro culture system that allows for significant re- invasion of target erythrocytes by Plasmodium vivax was used to study the role of the Duffy blood group antigen as a ligand for merozoite invasion by this human malaria species. Using human Duffy-positive and - negative erythrocytes, various primate erythrocytes, enzymatic modification of erythrocytes, and mAb that defines a new Duffy determinant (Fy6) we conclude that the erythrocyte glycoprotein carrying Duffy determinants...

  6. The conversion of group B red blood cells into group O by an alpha-D-galactosidase from taro (Colocasia esculenta).

    Science.gov (United States)

    Chien, S F; Lin-Chu, M

    1991-09-18

    An alpha-D-galactosidase (EC 3.2.1.21), capable of converting group B into group O red cells, was isolated from the stem portion of taro. It was purified about 3000 fold by gel filtration and ion-exchange chromatography. The blood group-converting activity was demonstrated by hemolysis and hemagglutination studies. This activity is comparable to that of alpha-D-galactosidase isolated from coffee beans. Taro alpha-D-galactosidase also hydrolyzes (1----4)- and (1----6)-linked alpha-D-galactopyranosyl groups from D-galactose-containing glycoconjugates. Taro alpha-D-galactosidase has a low Km value (0.28mM), a low molecular weight (40,000), and a neutral optimal pH (6.0). At a final enzyme concentration of 30 units/mL in the incubation mixture, the conversion of group B into group O activity was completed within two hours, without apparent changes in the shape of the red cells.

  7. Evidence of an association between the O blood group and allergic rhinitis

    OpenAIRE

    Nelson Falsarella; Ana Iara da Costa Ferreira; Fabiana Nakashima; Cinara de Cássia Brandão de Mattos; Luiz Carlos de Mattos

    2011-01-01

    OBJECTIVE: The aim of this study was to verify if ABO phenotypes are associated with allergic rhinitis. METHODS: 168 patients with allergic rhinitis and 168 control individuals from the same geographical region and paired by gender and age were enrolled in the study. ABO phenotypes were identified in red blood cells using the hemagglutination technique. The Fisher exact and chi-squared tests were employed to compare proportions. Statistical significance was set for an alpha error of 5% (p-val...

  8. Conformational energy calculations and proton nuclear overhauser enhancements reveal a unique conformation for blood group A oligosaccharides

    Energy Technology Data Exchange (ETDEWEB)

    Bush, C.A.; Yan, Z.Y.; Rao, B.N.N.

    1986-10-01

    The H NMR spectra of a series of blood group A active oligosaccharides containing from four to ten sugar residues have been completely assigned, and quantitative nuclear Overhauser enhancements (NOE) have been measured between protons separated by known distances within the pyranoside ring. The observation of NOE between anomeric protons and those of the aglycon sugar as well as small effects between protons of distant rings suggests that the oligosaccharides have well-defined conformations. Conformational energy calculations were carried out on a trisaccharide, Fuc( -1 2)(GalNAc( -1 3))-GalUS -O-me, which models the nonreducing terminal fragments of the blood group A oligosaccharides. The results of calculations with three different potential energy functions which have been widely used in peptides and carbohydrates gave several minimum energy conformations. In NOE calculations from conformational models, the rotational correlation time was adjusted to fit T1's and intra-ring NOE. Comparison of calculated maps of NOE as a function of glycosidic dihedral angles showed that only a small region of conformational space was consistent with experimental data on a blood group A tetrasaccharide alditol. This conformation occurs at an energy minimum in all three energy calculations. Temperature dependence of the NOE implies that the oligosaccharides adopt single rigid conformations which do not change with temperature.

  9. Conformational energy calculations and proton nuclear overhauser enhancements reveal a unique conformation for blood group A oligosaccharides

    International Nuclear Information System (INIS)

    The 1H NMR spectra of a series of blood group A active oligosaccharides containing from four to ten sugar residues have been completely assigned, and quantitative nuclear Overhauser enhancements (NOE) have been measured between protons separated by known distances within the pyranoside ring. The observation of NOE between anomeric protons and those of the aglycon sugar as well as small effects between protons of distant rings suggests that the oligosaccharides have well-defined conformations. Conformational energy calculations were carried out on a trisaccharide, Fuc(α-1→2)[GalNAc(α-1→3)]-Galβ-O-me, which models the nonreducing terminal fragments of the blood group A oligosaccharides. The results of calculations with three different potential energy functions which have been widely used in peptides and carbohydrates gave several minimum energy conformations. In NOE calculations from conformational models, the rotational correlation time was adjusted to fit T1's and intra-ring NOE. Comparison of calculated maps of NOE as a function of glycosidic dihedral angles showed that only a small region of conformational space was consistent with experimental data on a blood group A tetrasaccharide alditol. This conformation occurs at an energy minimum in all three energy calculations. Temperature dependence of the NOE implies that the oligosaccharides adopt single rigid conformations which do not change with temperature

  10. Biochemical identification of the bovine blood group M' antigen as a major histocompatibility complex class I-like molecule

    DEFF Research Database (Denmark)

    Hønberg, L S; Larsen, B; Koch, C;

    1995-01-01

    Absorption and elution experiments showed that it was impossible to separate antibodies against blood group factor M' from antibodies against bovine lymphocyte antigen (BoLA) A16 in an antiserum showing haemolytic activity against M' as well as lymphocytotoxic activity against BoLA-A16....... To elucidate the structural relationship between BoLA-A16 and blood group antigen M', immunoprecipitation experiments on red and white cell lysates isolated from M'-A16 positive and negative cattle were carried out. These results showed that M(r) 44,000 and M(r) 12000 polypeptides can be precipitated from both...... difference in the pI of the immunoprecipitable components of red and white cells was observed. All together, this indicates that either the blood group antigen M' is the BoLA-A16 class I antigen or M' and BoLA-A16 are two different class I polypeptides with the same relative mass, sharing identical epitopes...

  11. Incompatible insect technique: incompatible males from a Ceratitis capitata genetic sexing strain

    International Nuclear Information System (INIS)

    Wolbachia are obligatory intracellular and maternally inherited bacteria that infect and spread through natural arthropod populations by inducing male-killing, feminization, parthenogenesis and, most commonly, unidirectional and bidirectional cytoplasmic incompatibility (CI). Cytoplasmic incompatibility can be used to control natural populations of insect pests, in a way analogous to the sterile insect technique (SIT), namely through the incompatible insect technique (IIT). For the successful application of the ITT (based on a unidirectional CI approach) against a target species, it is essential that only males are released, as the release of females would lead to fertile matings between the released males and the released females and the establishment of a Wolbachia-carrying field population. In the present study, we describe a Wolbachia-infected line of the VIENNA 8 genetic sexing strain of the Mediterranean fruit fly, Ceratitis capitata (Wiedermann) (Diptera: Tephritidae), that carries the selectable marker temperature sensitive lethal (tsl). We show that (1) transferred Wolbachia induce high levels of CI even after the temperature treatment required for the male-only production, and (2) the Wolbachia-infected genetic sexing C. capitata line can be used in cage population suppression experiments analogous to the SIT. We also discuss our results in a comparison between IIT and SIT, investigating whether irradiation and cytoplasmic factors can be combined toward the development of novel strategies for insect pest control. (author)

  12. Next-Generation Sequencing for Antenatal Prediction of KEL1 Blood Group Status

    DEFF Research Database (Denmark)

    Rieneck, Klaus; Clausen, Frederik Banch; Dziegiel, Morten Hanefeld

    2015-01-01

    The KEL1 antigen can give rise to immunization of KEL2 mothers. Maternal antibodies can be transferred to the fetus and destroy fetal red blood cells and their stem cell precursors and give rise to serious fetal disease. It is important to be able to predict the fetal KEL status in order to inter......The KEL1 antigen can give rise to immunization of KEL2 mothers. Maternal antibodies can be transferred to the fetus and destroy fetal red blood cells and their stem cell precursors and give rise to serious fetal disease. It is important to be able to predict the fetal KEL status in order...... to intervene in those pregnancies where the fetus is at risk, and to ascertain when the fetus is not at risk. Technically it can be demanding to predict KEL1 status from a maternal blood sample. The KEL1 allele is based on a single SNP present in about 1-10 % of cell-free maternal DNA after gestation week 10...

  13. Adenoid and tonsil surgeries in children: How relevant is pre-operative blood grouping and cross-matching?

    Directory of Open Access Journals (Sweden)

    Lucky Onotai

    2013-01-01

    Full Text Available Background: As a part of pre-operative evaluation, several otolaryngologists group and cross-match blood routinely for children undergoing adenoid and tonsil surgeries. This practice has generated several debates either in support or against this practice. The aim of this study is to critically evaluate the incidence of post-tonsillectomy (with or without adenoidectomy bleeding and blood transfusions in otherwise healthy children with adenoid/tonsil pathologies conducted in the University of Port Harcourt Teaching Hospital (UPTH. Patients and Methods: A descriptive retrospective study of children who underwent adenoid and tonsil surgeries in the Department of Ear, Nose and Throat (ENT surgery of UPTH from January 2003 to December 2012. Children with family history of bleeding disorders and derangement of clotting profile as well as different co-morbidity like sickle cell disease were excluded from this study. The patients′ data were retrieved from the registers of ENT out-patient clinics, theatre registers and patients case notes. Demographic data, indications for surgery, preoperative investigations, complications and management outcomes were recorded and analyzed. Results: Out of 145 children that had adenoid and tonsil surgeries; only 100 met the criteria for this study. The study subjects included 65 males and 35 females (male: female ratio 1.9:1 belonging to 0-16 years age group (mean age: 3.46 ± 2.82 years. The age group of 3-5 years had the highest (n = 40, 40% number of surgeries. Adenotonsillectomy was the commonest (n = 85, 85% surgery performed on patients who had obstructive sleep apnea (OSA. The commonest (n = 6, 6% complication was haemorrhage, and only few (n = 3, 3% patients had blood transfusion. However, mortality was recorded in some (n = 3, 3% patients. Conclusion: This study confirms that the incidence of post adenoidectomy/tonsillectomy bleeding in otherwise healthy children is low and rarely requires blood transfusion

  14. Micro gel column technique is fit for detecting mixed fields post ABO incompatible hematopoietic stem cell transplantation.

    Science.gov (United States)

    Li, Min-Fang; Liu, Feng; Zhang, Min

    2015-04-01

    How to choose suitable serologic method for assessment of the actual stages of ABO chimera is more important to establish transfusion strategy for patients post-ABO incompatible hematopoietic stem cell transplantation. We reported ABO phenotypes of a patient post-ABO minor incompatible hematopoietic stem cell transplantation from 1+ weak agglutination by tube method was obviously reaffirmed to mixed fields with 4+ positive reaction by micro gel column card. Hence, blood bank technologists must continually work together with hematologist to establish appropriate transfusion strategy, and micro gel column technique can be more appropriate for detecting mixed fields during the whole period of transplantation. PMID:25578650

  15. Histo-blood group ABO antigen in oral potentially malignant lesions and squamous cell carcinoma--genotypic and phenotypic characterization

    DEFF Research Database (Denmark)

    Gao, Shan; Bennett, Erik Paul; Reibel, Jesper;

    2004-01-01

    Loss of histo-blood group A/B antigens is frequent in oral cancer. It is unclear whether this alteration is due to loss of the chromosomal region encoding the genes. The aim was to investigate genotypic alterations in the ABO locus in oral potentially malignant lesions and carcinomas. Seventy...... and 3/24 cases with mild and moderate dysplasia by genotyping analysis. O allele loss was found in 10 cases involving all four groups. In patients with heterozygous genotypes, A/B allelic loss by genotyping analysis was always followed by loss of A/B antigen expression by IHC staining. Loss of A...

  16. Evolution and Molecular Control of Hybrid Incompatibility in Plants

    Science.gov (United States)

    Chen, Chen; E, Zhiguo; Lin, Hong-Xuan

    2016-01-01

    Postzygotic reproductive isolation (RI) plays an important role in speciation. According to the stage at which it functions and the symptoms it displays, postzygotic RI can be called hybrid inviability, hybrid weakness or necrosis, hybrid sterility, or hybrid breakdown. In this review, we summarized new findings about hybrid incompatibilities in plants, most of which are from studies on Arabidopsis and rice. Recent progress suggests that hybrid incompatibility is a by-product of co-evolution either with “parasitic” selfish elements in the genome or with invasive microbes in the natural environment. We discuss the environmental influences on the expression of hybrid incompatibility and the possible effects of environment-dependent hybrid incompatibility on sympatric speciation. We also discuss the role of domestication on the evolution of hybrid incompatibilities. PMID:27563306

  17. West Nile Virus (WNV seroprevalence in a blood donors group of Milan

    Directory of Open Access Journals (Sweden)

    Giovanna Lunghi

    2012-09-01

    Full Text Available A seroprevalence study for anti West Nile virus was carried out among 864 healthy blood donors living in the metropolitan area of Milan by using a commercially available ELISA method. In addition, the performance of a novel ELISA assay for WNV antibodies was assessed. The sero-prevalence rate of WNV antibodies was 0.57% thus showing that WNV is likely circulating also in this up to now unknown area. The overall sensitivity and specificity of the novel ELISA were 99.9% and 45.4%, respectively, well comparable with that of the chosen reference immunoenzimatic method.

  18. Exposure and risk factors to coxiella burnetii, spotted fever group and typhus group Rickettsiae, and Bartonella henselae among volunteer blood donors in Namibia.

    Directory of Open Access Journals (Sweden)

    Bruce H Noden

    Full Text Available The role of pathogen-mediated febrile illness in sub-Saharan Africa is receiving more attention, especially in Southern Africa where four countries (including Namibia are actively working to eliminate malaria. With a high concentration of livestock and high rates of companion animal ownership, the influence of zoonotic bacterial diseases as causes of febrile illness in Namibia remains unknown.The aim of the study was to evaluate exposure to Coxiella burnetii, spotted fever and typhus group rickettsiae, and Bartonella henselae using IFA and ELISA (IgG in serum collected from 319 volunteer blood donors identified by the Blood Transfusion Service of Namibia (NAMBTS. Serum samples were linked to a basic questionnaire to identify possible risk factors. The majority of the participants (64.8% had extensive exposure to rural areas or farms. Results indicated a C. burnetii prevalence of 26.1% (screening titre 1∶16, and prevalence rates of 11.9% and 14.9% (screening titre 1∶100 for spotted fever group and typhus group rickettsiae, respectively. There was a significant spatial association between C. burnetii exposure and place of residence in southern Namibia (P0.012, especially cattle (P>0.006, were also significantly associated with C. burnetii exposure. Males were significantly more likely than females to have been exposed to spotted fever (P<0.013 and typhus (P<0.011 group rickettsiae. Three (2.9% samples were positive for B. henselae possibly indicating low levels of exposure to a pathogen never reported in Namibia.These results indicate that Namibians are exposed to pathogenic fever-causing bacteria, most of which have flea or tick vectors/reservoirs. The epidemiology of febrile illnesses in Namibia needs further evaluation in order to develop comprehensive local diagnostic and treatment algorithms.

  19. The breed prevalence of Dog Erythrocyte Antigen 1.1 in the Onderstepoort area of South Africa and its significance in selection of canine blood donors

    OpenAIRE

    L.L. Van der Merwe; L.S. Jacobson; J.G. Pretorius

    2002-01-01

    The blood group antigen Dog Erythrocyte Antigen (DEA) 1.1 is clinically the most important canine blood group as DEA 1.1 antibodies are capable of causing acute haemolytic, potentially life-threatening transfusion reactions. Dogs do not have naturally occurring antibodies to DEA 1.1 but are rapidly sensitised by the first incompatible transfusion. The prevalence of DEA 1.1 in the general dog population is estimated at 42-46 %. Canine blood donors registered with the Onderstepoort Animal Blood...

  20. Mechanisms of protein sequence divergence and incompatibility.

    Directory of Open Access Journals (Sweden)

    Alon Wellner

    Full Text Available Alignments of orthologous protein sequences convey a complex picture. Some positions are utterly conserved whilst others have diverged to variable degrees. Amongst the latter, many are non-exchangeable between extant sequences. How do functionally critical and highly conserved residues diverge? Why and how did these exchanges become incompatible within contemporary sequences? Our model is phosphoglycerate kinase (PGK, where lysine 219 is an essential active-site residue completely conserved throughout Eukaryota and Bacteria, and serine is found only in archaeal PGKs. Contemporary sequences tested exhibited complete loss of function upon exchanges at 219. However, a directed evolution experiment revealed that two mutations were sufficient for human PGK to become functional with serine at position 219. These two mutations made position 219 permissive not only for serine and lysine, but also to a range of other amino acids seen in archaeal PGKs. The identified trajectories that enabled exchanges at 219 show marked sign epistasis - a relatively small loss of function with respect to one amino acid (lysine versus a large gain with another (serine, and other amino acids. Our findings support the view that, as theoretically described, the trajectories underlining the divergence of critical positions are dominated by sign epistatic interactions. Such trajectories are an outcome of rare mutational combinations. Nonetheless, as suggested by the laboratory enabled K219S exchange, given enough time and variability in selection levels, even utterly conserved and functionally essential residues may change.

  1. Blood Group Discrepancy-First Sign of Autoimmune Hemolytic Anemia after Hematopoietic Stem Cell Transplantation in a Child.

    Science.gov (United States)

    Datta, Suvro Sankha; Reddy, Mahua; Basu, Sabita; Krishnan, Shekhar

    2016-06-01

    A 12-year-old male child was presented in the emergency with features of anemia and mild icterus on day+67 of HSCT. The child was suffering from Fanconi anemia and undergone HSCT from ABO-matched, fully HLA matched sibling donor. The diagnosis of mixed type AIHA due to cytomegalovirus reactivation was made in the immunohematology laboratory and blood group discrepancy was the first sign of AIHA in this patient. Though the cold agglutinin titer was not significant but the clinical symptoms and laboratory evidences were suggestive of significant hemolysis due to underlying IgG autoantibody. In addition the high complement avidity of IgM autoantibody might also be a contributing factor for clinically significant hemolysis in this case. The patient was successfully treated with phenotype matched blood transfusion, rituximab and oral steroid therapy. PMID:27408394

  2. DYNAMICS OF INSULIN-LIKE GROWTH FACTOR-1 (IGF-1 IN CHILDREN AFTER AB0-INCOMPATIBLE LIVER TRANSPLANTATION

    Directory of Open Access Journals (Sweden)

    O.P. Shevchenko

    2014-01-01

    Full Text Available It is shown that liver transplantation (LT from donor with incompatible blood type (AB0i may be effective and safe, but the impact of such operation upon the various systems of the body has not been investigated yet. Insulinlike growth factor-1 (IGF-1 is synthesized in the liver and mediates the action of growth hormone. The level of IGF-1 is a marker of the processes of cell proliferation and tissue regeneration. Aim. To evaluate levels of IGF-1 in children-recipients with liver transplant from AB0i (incompatible and AB0c (compatible donors.Materials and methods. 140 children aged 3 to 36 (19,5 ± 16,5 months with congenital diseases of the hepatobiliar system, 58 of them boys, were surveyed. All patients underwent transplantation of left lateral liver sector from living related donors: 111 children were transplanted with fragment of the liver from AB0c donors, 29 – from AB0i donors; in 10 children with AB0i liver before and/or after LT operation anti-group antibodies (anti-A/B were revealed. The concentration of IGF-1 was determined by ELISA using specifi c kits (Immunodiagnostic System, USA in samples of blood plasma, which were received up to a month and a year after a liver transplant.Results. Average level of IGF-1 21,0 ± 29,5 μg/l in patients before LT was signifi cantly lower than in healthy children (52,2 ± 26,3 μg/l, p < 0,001 and did not vary in children, having received later a piece of liver from a compatible (AB0c donor and from donor AB0i (23,5 ± 30,9 and 21,2 ± 23,2 μg/l respectively, p = 0,70. In patients with anti-A/B prior to surgery average level of IGF-1 was not different from that of the patients without antibodies (32,6 ± 27,6 and 22,3 ± 29,6 μg/l respectively, p = 0,4. One month after LT level of IGF-1 has increased both in the general group, and in patients with AB0c and AV0i liver (92,1 ± 77,8 and 131,2 ± 106,7 μg/l respectively, p = 0,09. The level of IGF-1 was not varied in the group with antibodies

  3. [The biological significance of the genetically determined Se-se human blood group and its effect on the antibody formation process in donors immunized with staphylococcal anatoxin].

    Science.gov (United States)

    Patoka, V V

    1999-01-01

    82 blood donors have been observed, 63 of them were immunized. Blood group ABO(H), secreting group Se--se and Staphylococcus antibody contents (anti-alpha-staphylolysins) were determined in all the donors. It was found out that the donors-secretors with A(II) blood group exhibited the antibody-production increasing. It is supposed that the secreting of group-specific substance A, that has structural elements similar those of staphylococcus into saliva promotes antibody production increase against staphylococcus. The mechanism of such specific stimulation remains to be unknown and requires further studying. PMID:10687067

  4. Quantitatively determined self-incompatibility : 1. Theoretical considerations.

    Science.gov (United States)

    Mayo, O; Leach, C R

    1989-03-01

    It has been reported that incomplete self-incompatibility could be determined in Borago officinalis by many genes. Simple ten-gene models for such enforced cross-fertilization have been developed and their properties examined by computer simulation. Mutation rates necessary to maintain a given level of variability in small populations are high, as already determined theoretically for oligogenic self-incompatibility systems. However, the extent of ineffective pollination is very much greater in the ten-gene system. This finding may be verifiable in borage if it is indeed self-incompatible. PMID:24232615

  5. Dobzhansky-Muller incompatibilities and adaptation to a shared environment

    Science.gov (United States)

    Orr, H. Allen

    2009-01-01

    Natural selection might drive the evolution of postzygotic reproductive isolation even when allopatric populations adapt to identical environments, an idea first suggested by Muller (1942). Here we analyze this scenario mathematically, focusing on the evolution of a Dobzhansky-Muller incompatibility between populations. Our results identify a potential problem with Muller’s scenario: adaptation to identical environments can often involve substitution of the same alleles, precluding formation of a hybrid incompatibility. Our results show that the probability of evolving a Dobzhansky-Muller incompatibility falls as selection coefficients among beneficial alleles become less similar. PMID:19142201

  6. Control of Blood Pressure and Risk Attenuation: Post Trial Follow-Up of Randomized Groups.

    Directory of Open Access Journals (Sweden)

    Tazeen H Jafar

    Full Text Available Evidence on long term effectiveness of public health strategies for lowering blood pressure (BP is scarce. In the Control of Blood Pressure and Risk Attenuation (COBRA Trial, a 2 x 2 factorial, cluster randomized controlled trial, the combined home health education (HHE and trained general practitioner (GP intervention delivered over 2 years was more effective than no intervention (usual care in lowering systolic BP among adults with hypertension in urban Pakistan. However, it was not clear whether the effect would be sustained after the cessation of intervention. We conducted 7 years follow-up inclusive of 5 years of post intervention period of COBRA trial participants to assess the effectiveness of the interventions on BP during extended follow-up.A total of 1341 individuals 40 years or older with hypertension (systolic BP 140 mm Hg or greater, diastolic BP 90 mm Hg or greater, or already receiving treatment were followed by trained research staff masked to randomization status. BP was measured thrice with a calibrated automated device (Omron HEM-737 IntelliSense in the sitting position after 5 minutes of rest. BP measurements were repeated after two weeks. Generalized estimating equations (GEE were used to analyze the primary outcome of change in systolic BP from baseline to 7- year follow-up. The multivariable model was adjusted for clustering, age at baseline, sex, baseline systolic and diastolic BP, and presence of diabetes.After 7 years of follow-up, systolic BP levels among those randomised to combined HHE plus trained GP intervention were significantly lower (2.1 [4.1-0.1] mm Hg compared to those randomised to usual care, (P = 0.04. Participants receiving the combined intervention compared to usual care had a greater reduction in LDL-cholesterol (2.7 [4.8 to 0.6] mg/dl.The benefit in systolic BP reduction observed in the original cohort assigned to the combined intervention was attenuated but still evident at 7- year follow-up. These

  7. Brominated flame retardants and perfluorinated chemicals, two groups of persistent contaminants in Belgian human blood and milk

    Energy Technology Data Exchange (ETDEWEB)

    Roosens, Laurence [Toxicological Centre, Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk (Belgium); D' Hollander, Wendy; Bervoets, Lieven [Laboratory for Ecophysiology, Biochemistry and Toxicology, Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp (Belgium); Reynders, Hans; Van Campenhout, Karen [Environment and Health Unit, Department of Environment, Nature and Energy, Flemish Government - Koning Albert II-laan 20, Bus 8, 1000 Brussels (Belgium); Cornelis, Christa; Van Den Heuvel, Rosette; Koppen, Gudrun [Unit Environmental Risk and Health, Flemish Institute of Technological Research (VITO), Boeretang 200, 2400 Mol (Belgium); Covaci, Adrian, E-mail: adrian.covaci@ua.ac.b [Toxicological Centre, Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk (Belgium); Laboratory for Ecophysiology, Biochemistry and Toxicology, Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp (Belgium)

    2010-08-15

    We assessed the exposure of the Flemish population to brominated flame retardants (BFRs) and perfluorinated compounds (PFCs) by analysis of pooled cord blood, adolescent and adult serum, and human milk. Levels of polybrominated diphenyl ethers (PBDEs) in blood (range 1.6-6.5 ng/g lipid weight, lw) and milk (range 2.0-6.4 ng/g lw) agreed with European data. Hexabromocyclododecane ranged between <2.1-5.7 ng/g lw in milk. Perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) dominated in blood and ranged between 1 and 171 ng/mL and <0.9-9.5 ng/mL, respectively. Total PFC levels in milk ranged between <0.5-29 ng/mL. A significant increase in PBDE concentrations was detected from newborns (median 2.1) to the adolescents and adults (medians 3.8 and 4.6 ng/g lw, respectively). An identical trend was observed for PFOS, but not for PFOA. We estimated that newborn exposure to BFRs and PFCs occurs predominantly post-natally, whereas placental transfer has a minor impact on the body burden. - The exposure to BFRs and PFCs of general Flemish population has been assessed throughout several age groups.

  8. Evaluating Blood Parameters, P53, and IL6 in Personnel of Copper Complex: A Comparison with Control Group

    Directory of Open Access Journals (Sweden)

    Hadis Ahmadiraad

    2016-03-01

    Full Text Available Background & Objectives: Industrial pollution including trace elements is the ability to exert many biological effects such as cancer and inflammatory diseases on humans. Therefore, in this study, some of the inflammation and cancer awareness factors such as P53 and IL6 and some blood indices are examined along with trace elements to which people are normally exposed. Materials & Methods: The population includes 45 workers subjected to trace elements who are studied in comparison with the control group with some biochemical parameters such as WBC, RBC, and CRP. In addition, gene expressions of p53 and IL6 are measured by Real time PCR technique. Results: The results show that the gene expressions of IL6 and P53 increases significantly (P –Value p53=0.00, IL6=0.0037. Furthermore, the number of red and white blood cells demonstrate a substantial upsurge. The level of liver enzymes of ALT and AST grows. Additionally, ALP reduces and CRP is negative in all the subjects. (P = 0.001. Conclusion: The results confirm that industrial pollution is able to induce some changes in gene expressions of P53, IL6, and some blood parameters. It may create serious risks for people who will be exposed to pollution in the future.

  9. Breaking Gaussian incompatibility on continuous variable quantum systems

    International Nuclear Information System (INIS)

    We characterise Gaussian quantum channels that are Gaussian incompatibility breaking, that is, transform every set of Gaussian measurements into a set obtainable from a joint Gaussian observable via Gaussian postprocessing. Such channels represent local noise which renders measurements useless for Gaussian EPR-steering, providing the appropriate generalisation of entanglement breaking channels for this scenario. Understanding the structure of Gaussian incompatibility breaking channels contributes to the resource theory of noisy continuous variable quantum information protocols

  10. Role of Subgroup Incompatibility in Newborn Jaundice Requiring Exchange Transfusion

    OpenAIRE

    Annagür, Ali; Altunhan, Hüseyin; Konak, Murat; Koç, Hasan; Örs, Rahmi

    2014-01-01

    We aimed to determine the role of exchange transfusion related complications, treatment, and etiology as well as subgroup incompatibility in patients subject to ET (exchange transfusion) due to newborn jaundice. 82 patients hospitalized due to newborn jaundice and exposed to exchange transfusion between August 2007 and August 2011 were retrospectively studied. Before ET mean total serum bilirubin was 29,2±9,83. The most frequent cause of ET was ABO incompatibility (31%) followed by...

  11. Breaking Gaussian incompatibility on continuous variable quantum systems

    Energy Technology Data Exchange (ETDEWEB)

    Heinosaari, Teiko, E-mail: teiko.heinosaari@utu.fi [Turku Centre for Quantum Physics, Department of Physics and Astronomy, University of Turku, FI-20014 Turku (Finland); Kiukas, Jukka, E-mail: jukka.kiukas@aber.ac.uk [Department of Mathematics, Aberystwyth University, Penglais, Aberystwyth, SY23 3BZ (United Kingdom); Schultz, Jussi, E-mail: jussi.schultz@gmail.com [Turku Centre for Quantum Physics, Department of Physics and Astronomy, University of Turku, FI-20014 Turku (Finland); Dipartimento di Matematica, Politecnico di Milano, Piazza Leonardo da Vinci 32, I-20133 Milano (Italy)

    2015-08-15

    We characterise Gaussian quantum channels that are Gaussian incompatibility breaking, that is, transform every set of Gaussian measurements into a set obtainable from a joint Gaussian observable via Gaussian postprocessing. Such channels represent local noise which renders measurements useless for Gaussian EPR-steering, providing the appropriate generalisation of entanglement breaking channels for this scenario. Understanding the structure of Gaussian incompatibility breaking channels contributes to the resource theory of noisy continuous variable quantum information protocols.

  12. Vegetative incompatibility among monoconidial isolates of Bipolaris sorokiniana.

    Science.gov (United States)

    Poloni, A; Pessi, I S; Frazzon, A P G; Van Der Sand, S T

    2009-02-01

    Bipolaris sorokiniana is a phytopathogenic fungus that causes diseases of cereal crops, such as leaf-spot disease, common root rot, and black point of grain. Because of its great morphological, physiological, and genetic variability, this fungus is difficult to control. The aim of this investigation was to study the variability of isolates of B. sorokiniana by means of vegetative incompatibility. Thirty-five isolates of B. sorokiniana from different geographical regions in Brazil and other countries were used. The vegetative incompatibility between the isolates and the influences of different culture media on these reactions were evaluated. The total protein profile of the isolates was analyzed when the isolates were cultured separately, and in cultures of compatibility and incompatibility reactions. Eighteen of 31 confrontations showed vegetative incompatibility. The results obtained with different culture media for the vegetative compatibility/incompatibility genotypes suggested that the type of substratum influences these reactions. No differences in protein profiles among the isolates were observed. This result suggests that there is no induction of expression of different proteins in vegetative incompatibility reactions.

  13. Genetic evidence that two independent S-loci control RNase-based self-incompatibility in diploid strawberry.

    Science.gov (United States)

    Bosković, Radovan I; Sargent, Daniel J; Tobutt, Kenneth R

    2010-03-01

    The self-incompatibility mechanism that reduces inbreeding in many plants of the Rosaceae is attributed to a multi-allelic S locus which, in the Prunoideae and Maloideae subfamilies, comprises two complementary genes, a stylar-expressed S-RNase and a pollen-expressed SFB. To elucidate incompatibility in the subfamily Rosoideae, stylar-specific RNases and self-(in)compatibility status were analysed in various diploid strawberries, especially Fragaria nubicola and F. viridis, both self-incompatible, and F. vesca, self-compatible, and in various progenies derived from them. Unexpectedly, two unlinked RNase loci, S and T, were found, encoding peptides distinct from Prunoideae and Maloideae S-RNases; the presence of a single active allele at either is sufficient to confer self-incompatibility. By contrast, in diploid Maloideae and Prunoideae a single locus encodes S-RNases that share several conserved regions and two active alleles are required for self-incompatibility. Our evidence implicates the S locus in unilateral inter-specific incompatibility and shows that S and T RNases can, remarkably, confer not only allele-specific rejection of cognate pollen but also unspecific rejection of Sn Tn pollen, where n indicates a null allele, consistent with the the presence of the pollen component, SFB, activating the cognitive function of these RNases. Comparison of relevant linkage groups between Fragaria and Prunus suggests that Prunus S-RNases, unique in having two introns, may have resulted from gene conversion in an ancestor of Prunus. In addition, it is shown that there is a non-S locus that is essential for self-incompatibility in diploid Fragaria.

  14. Examination of the Blood Groups and Biologal Activity of the Freeze-drying Red Blood Cells%冻干前后红细胞血型及生物活性指标的检测

    Institute of Scientific and Technical Information of China (English)

    吴学忠; 刘忠; 吕蓉; 李敏; 李素萍; 於娟; 赵丹

    2011-01-01

    目的 检测冻干前、后红细胞血型抗原及其生物学活性,了解冻干前、后红细胞血型抗原及其生物学活性的变化情况.方法红细胞血型抗原的检测采用血型血清学方法,2,3-DPG和ATP用ELISA法.结果在ABO、Rh、MNSs、Kell、Duffy、P血型抗原中,冷冻前、冻干后红细胞血型抗原一致;在Lewis血型抗原中,冷冻前、冻干后红细胞血型抗原有变化.冷冻前、冻干后红细胞2,3-DPG和ATP含量均无明显变化.结论冷冻前、冻干后红细胞Lewis血型系统中的Lea和Leb血型抗原变化有明显差异;冷冻前、冻干后红细胞2,3-DPG和ATP含量变化无统计学意义.%Objective To detect the blood group antigens, the levels of 2,3-DPG and ATP of red blood cells (RBCs) before and after freeze-drying. Also the changes of blood group antigens and biological activity of RBCs before and after RBCs freeze-drying was observed. Methods The blood group antigens of RBCs were detected by the blood group serology, the levels of 2,3-DPG and ATP were detected by ELISA. Results The blood group antigens of RBCs were the same as before and after freeze-drying about ABO, Rh, MNSs, Kell, Duffy and P blood group systems. But the blood group antigens of RBCs of Lewis blood group systems were different before and after freeze-drying. The levels of 2,3-DPG and ATP of RBCs before and after freeze-drying didn't change significantly. Conclusion The Lea and Leb blood group antigens of RBCs before and after freeze-drying of Lewis blood group systems have changed significantly. The levels of 2,3-DPG and ATP of RBCs before and after freeze-drying havn't chenged significantly.

  15. Adherence to an occupational blood borne pathogens exposure management program among healthcare workers and other groups at risk in Argentina

    Directory of Open Access Journals (Sweden)

    Marisa Miceli

    2005-12-01

    Full Text Available We conducted a retrospective review of 130 occupational blood borne pathogens exposure (BBP-OE records at Centro de Estudios Médicos e Investigaciones Clínicas, a university hospital with an ongoing educational program and a postexposure management program for healthcare workers (HCWs since 1995, in order to evaluate adherence to a hospital BBP-OE management program. We compared HCWs from our institution (Group 1 and HCWs from independent institutions that contract our postexposure management program (Group 2. Compliance with standard precautions in Group 1 was inadequate in 77%, 23%, and 16% of nurses, physicians, and others, respectively. A greater proportion of HCWs in Group 1 (74% vs. 40% reported occupational accidents within two hours after exposure (p = 0.0001. No difference was observed regarding compliance with adherence to schedule, partial adherence, and loss at follow-up (14%, 33%, and 53%; p > 0.05. Adherence to the standard of care for BBP-OE, including postexposure prophylaxis, was low (HIV: 53% and HBV: 63%. Knowledge of the seropositive status of the source patient did not improve adherence. We conclude that postexposure programs do not guarantee appropriate behavior by HCWs. General interventions and ongoing personnel education to modify individual attitudes are needed, as are continued efforts to assess HCWs' experiences with these programs, as well as the identification of strategies to improve adherence.

  16. Distribution of von Willebrand factor levels in young women with and without bleeding symptoms: influence of ABO blood group and promoter haplotypes

    DEFF Research Database (Denmark)

    Lethagen, S.; Hillarp, A.; Ekholm, C.;

    2008-01-01

    . It was the objective of the present study to evaluate the distribution of VWF levels in young females with or without bleeding symptoms in this population, and the influence of ABO blood group and promoter haplotypes on VWF levels and to identify a possible increased prevalence of VWD in females with bleeding symptoms.......4%) (p = 0.017). Blood group O was found in 14/18 girls with low VWF:RCo. There was a highly significant correlation between VWF:RCo and blood group O and non-O genotypes. Two common VWF promoter haplotypes did not contribute to the VWF:RCo variation. VWF levels did not correlate with time during...... menstrual cycle, or the use of oral contraceptives. No case fulfilled the diagnostic criteria for VWD. In conclusion, low VWF:RCo was significantly more frequent in females with bleeding symptoms. However, we found no case fulfilling strict diagnostic criteria for VWD. The ABO blood group was a strong...

  17. Molecular screening for Candida orthopsilosis and Candida metapsilosis among Danish Candida parapsilosis group blood culture isolates: proposal of a new RFLP profile for differentiation

    DEFF Research Database (Denmark)

    Mirhendi, Hossein; Bruun, Brita; Schønheyder, Henrik Carl;

    2010-01-01

    Candida orthopsilosis and Candida metapsilosis are recently described species phenotypically indistinguishable from Candida parapsilosis . We evaluated phenotyping and molecular methods for the detection of these species among 79 unique blood culture isolates of the C. parapsilosis group obtained...

  18. Distribution of ABO blood groups and rhesus factor in a Large Scale Study of different cities and ethnicities in Khuzestan province, Iran

    Directory of Open Access Journals (Sweden)

    J. Torabizade maatoghi

    2016-01-01

    Conclusion: Our study showed ethnicity-related prevalence. Overall, the blood group O had the highest prevalence and AB the lowest percentage among the ethnicities, indicating a significant difference with studies in other parts of the world.

  19. ABO血型鉴定不一致原因分析及解决方法%ABO Blood Group Identification ;not Consistent Cause Analysis

    Institute of Scientific and Technical Information of China (English)

    万会林

    2013-01-01

    目的:分析ABO血型鉴定不一致原因分析及解决方法方法:应用ABO血型鉴定原理即根据红细胞表面有无A抗原和(或)B抗原,将血型分为A型、B型、AB型及O型4种,可利用红细胞的凝集实验,通过正反定型准确鉴定ABO血型。结果:笔者就几年血型不一致的原因进行分析,并加以分别探讨。结论:正确鉴定ABO血型是输血的关键之一,误输异型血可使受血者发生严重的输血反应,危及病人生命安全。对于ABO血型鉴定不一致的,无法判断的特殊血型标本,可以针对其原因,采取多种方法正确鉴定ABO血型。%objective:To analyse ABO blood group identifica-tion not consistent cause analysis and solution method:the applica-tion of ABO blood group identification principle according to red blood cells that have A surface antigen and (or) B antigens, will be di-vided into A blood type, type B, type AB and type O four, can use of red blood cells agglutinate experiment, through the positive and neg-ative stereotypes accurate identification ABO blood group. Results:the author is not consistent with a few years blood type analysis of the causes, and to study. Conclusion:correctly identify the ABO blood group is one of the keys to a blood transfusion, abnormal blood can make false input by the blood transfusion reactions happen serious, endanger patient safety. For the ABO blood group appraisal does not agree, can't judge blood type specimens of the special, can't judge of the special specimens, can according to the cause and take DuoZhong method correctly identify the ABO blood group.

  20. Abnormal hemoglobin genotypes and ABO and rhesus blood groups associated with HIV infection among HIV-exposed infants in North Western Nigeria

    Directory of Open Access Journals (Sweden)

    Buseri FI

    2014-06-01

    Full Text Available Fiekumo I Buseri,1 Charity N Okonkwo21Hematology and Blood Transfusion Science Unit, Department of Medical Laboratory Science, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria; 2Department of Medical Laboratory Sciences, Rivers State University of Science and Technology, Port Harcourt, NigeriaBackground: Hemoglobin genotypes and blood groups have been known to be associated with diseases, but the relationship with human immunodeficiency virus (HIV infection among Nigerian infants is not well known.Objective: This study aims to determine the association between hemoglobin genotypes and blood groups with HIV infection among HIV-exposed Nigerian infants.Methods: This cross-sectional study examined 312 HIV-exposed infants (aged 8–16 months in Sokoto State, Nigeria. HIV screening was performed using the HIV DNA polymerase chain reaction technique on dried blood spots. Hemoglobin electrophoresis and ABO and Rhesus (Rh blood groups were carried out using standard techniques.Results: This study found 20.5% HIV-1 seropositivity among the infants, with 20.9% of males and 20.1% of females positive for HIV-1. Babies' sex and HIV seropositivity was not significant (χ2=0.27, df=1, P=0.869. The blood group distribution was O (43.3%, A (36.8%, B (15.7%, AB (4.2%, RhD+ (95.6%, and RhD– (4.4%. The combined ABO and Rh blood groups among the study population were O+ (40.1%, A+ (36.2%, B+ (15.1%, AB+ (4.2%, O– (3.2%, A– (0.6%, and B– (0.6%. No AB– baby was found. The association between blood groups and HIV seropositivity was not significant (Fisher’s exact test =9.140; P=0.169; however, group AB+ showed the highest probable association with HIV seropositivity (46.2%, followed by A+ (23.9%. The prevalence of hemoglobin genotypes was AA (71.5%, AS (25.3%, AC (2.2%, and SC (1.0%. Hemoglobin SS and other hemoglobin variants were not found. A significant association (χ2=8.432, df=3, P=0.034 was observed between SC and HIV-1 infection

  1. Noise robustness of the incompatibility of quantum measurements

    Science.gov (United States)

    Heinosaari, Teiko; Kiukas, Jukka; Reitzner, Daniel

    2015-08-01

    The existence of incompatible measurements is a fundamental phenomenon having no explanation in classical physics. Intuitively, one considers given measurements to be incompatible within a framework of a physical theory, if their simultaneous implementation on a single physical device is prohibited by the theory itself. In the mathematical language of quantum theory, measurements are described by POVMs (positive operator valued measures), and given POVMs are by definition incompatible if they cannot be obtained via coarse-graining from a single common POVM; this notion generalizes noncommutativity of projective measurements. In quantum theory, incompatibility can be regarded as a resource necessary for manifesting phenomena such as Clauser-Horne-Shimony-Holt (CHSH) Bell inequality violations or Einstein-Podolsky-Rosen (EPR) steering which do not have classical explanation. We define operational ways of quantifying this resource via the amount of added classical noise needed to render the measurements compatible, i.e., useless as a resource. In analogy to entanglement measures, we generalize this idea by introducing the concept of incompatibility measure, which is monotone in local operations. In this paper, we restrict our consideration to binary measurements, which are already sufficient to explicitly demonstrate nontrivial features of the theory. In particular, we construct a family of incompatibility monotones operationally quantifying violations of certain scaled versions of the CHSH Bell inequality, prove that they can be computed via a semidefinite program, and show how the noise-based quantities arise as special cases. We also determine maximal violations of the new inequalities, demonstrating how Tsirelson's bound appears as a special case. The resource aspect is further motivated by simple quantum protocols where our incompatibility monotones appear as relevant figures of merit.

  2. Studies of blood groups and protein polymorphisms in the Brazilian horse breeds Mangalarga Marchador and Mangalarga (Equus caballus

    Directory of Open Access Journals (Sweden)

    Andréia Samaha Lippi

    2003-12-01

    Full Text Available Allelic frequencies at 12 loci (five blood groups: C, D, K, P, and U; and seven protein polymorphisms: Al, A1B, Es, Gc, Hb, PGD, and Tf, are given for two Brazilian horse breeds: Mangalarga Marchador and Mangalarga. The high genetic identity value found (96.0% is consistent with their common origin, although, at some point of the development of Mangalarga Marchador, Mangalarga separated from the original stock. The expected average heterozygosity was higher in Mangalarga Marchador. The populations presented genetic differentiation, as shown by the statistically significant value of F ST. The nonsignificant F IS values showed that there was no appreciable consanguineous mating in any of the two populations. Exclusion probability calculated for the 12 loci was 87.0% and 86.5% for Mangalarga Marchador and Mangalarga, respectively. No genetic equilibrium was observed in the A1B, Tf, and Es loci of Mangalarga Marchador. The frequencies of blood factors A, Q, and T were calculated.

  3. Fetal blood grouping using cell free DNA - an improved service for RhD negative pregnant women.

    Science.gov (United States)

    Bills, V L; Soothill, P W

    2014-04-01

    Red cell alloimmunisation involves the transplacental movement of maternally derived red cell antibodies into the fetal circulation, causing red cell haemolysis, fetal anaemia and ultimately fetal death. Current standard UK practice is to prevent sensitisation to the D antigen by administering anti-D at about 28 weeks' gestation to all RhD negative pregnancies. The determination of fetal blood group by non-invasive cell free fetal DNA testing offers an improved and more efficient service to RhD negative pregnant women and avoids the potential iatrogenic harm associated with standard practice. It also has significantly improved the management of women with red cell alloimunisation to D and other antigens. This review summarises the past and future management of red cell alloimmunisation during pregnancy and the impact of ffDNA tests. PMID:24679596

  4. Estimation of nonpaternity in the Mexican population of Nuevo Leon: a validation study with blood group markers.

    Science.gov (United States)

    Cerda-Flores, R M; Barton, S A; Marty-Gonzalez, L F; Rivas, F; Chakraborty, R

    1999-07-01

    A method for estimating the general rate of nonpaternity in a population was validated using phenotype data on seven blood groups (A1A2BO, MNSs, Rh, Duffy, Lutheran, Kidd, and P) on 396 mother, child, and legal father trios from Nuevo León, Mexico. In all, 32 legal fathers were excluded as the possible father based on genetic exclusions at one or more loci (combined average exclusion probability of 0.694 for specific mother-child phenotype pairs). The maximum likelihood estimate of the general nonpaternity rate in the population was 0.118 +/- 0.020. The nonpaternity rates in Nuevo León were also seen to be inversely related with the socioeconomic status of the families, i.e., the highest in the low and the lowest in the high socioeconomic class. We further argue that with the moderately low (69.4%) power of exclusion for these seven blood group systems, the traditional critical values of paternity index (PI > or = 19) were not good indicators of true paternity, since a considerable fraction (307/364) of nonexcluded legal fathers had a paternity index below 19 based on the seven markers. Implications of these results in the context of genetic-epidemiological studies as well as for detection of true fathers for child-support adjudications are discussed, implying the need to employ a battery of genetic markers (possibly DNA-based tests) that yield a higher power of exclusion. We conclude that even though DNA markers are more informative, the probabilistic approach developed here would still be needed to estimate the true rate of nonpaternity in a population or to evaluate the precision of detecting true fathers. PMID:10407460

  5. Molecular Characterization of the Cytidine Monophosphate-N-Acetylneuraminic Acid Hydroxylase (CMAH) Gene Associated with the Feline AB Blood Group System

    Science.gov (United States)

    Tada, Naomi; Ochiai, Kazuhiko; Chong, Yong Hwa; Kato, Yuiko; Mitsui, Hiroko; Gin, Azusa; Oda, Hitomi; Azakami, Daigo; Tamura, Kyoichi; Sako, Toshinori; Inagaki, Takeshi; Sakamoto, Atsushi; Tsutsui, Toshihiko; Bonkobara, Makoto; Tsuchida, Shuichi; Ikemoto, Shigenori

    2016-01-01

    Cat’s AB blood group system (blood types A, B, and AB) is of major importance in feline transfusion medicine. Type A and type B antigens are Neu5Gc and Neu5Ac, respectively, and the enzyme CMAH participating in the synthesis of Neu5Gc from Neu5Ac is associated with this cat blood group system. Rare type AB erythrocytes express both Neu5Gc and Neu5Ac. Cat serum contains naturally occurring antibodies against antigens occurring in the other blood types. To understand the molecular genetic basis of this blood group system, we investigated the distribution of AB blood group antigens, CMAH gene structure, mutation, diplotypes, and haplotypes of the cat CMAH genes. Blood-typing revealed that 734 of the cats analyzed type A (95.1%), 38 cats were type B (4.9%), and none were type AB. A family of three Ragdoll cats including two type AB cats and one type A was also used in this study. CMAH sequence analyses showed that the CMAH protein was generated from two mRNA isoforms differing in exon 1. Analyses of the nucleotide sequences of the 16 exons including the coding region of CMAH examined in the 34 type B cats and in the family of type AB cats carried the CMAH variants, and revealed multiple novel diplotypes comprising several polymorphisms. Haplotype inference, which was focused on non-synonymous SNPs revealed that eight haplotypes carried one to four mutations in CMAH, and all cats with type B (n = 34) and AB (n = 2) blood carried two alleles derived from the mutated CMAH gene. These results suggested that double haploids selected from multiple recessive alleles in the cat CMAH loci were highly associated with the expression of the Neu5Ac on erythrocyte membrane in types B and AB of the feline AB blood group system. PMID:27755584

  6. 羊水 ABH血型物质测定与 ABO 血型基因分型%Detection of amniotic fluid ABH blood group substances and ABO blood type gene classification

    Institute of Scientific and Technical Information of China (English)

    陈江; 逯心敏; 郭渝; 胡伟

    2014-01-01

    Objective To detect amniotic fluid ABH blood group substances and ABO blood group genotype by the polymerase chain reaction with sequence-specific primers(PCR-SSP) to increase the prenatal diagnosis of fetal ABO blood group .Methods 53 pregnant women with gestational age 16 -25 weeks were selected .Amniotic fluid was extracted for detecting ABH blood group substances by the serological indirect agglutinating reaction ;the amniotic fluid cells were separated for extracting DNA .Then the PCR-SSP technique was adopted to analyze the ABO blood group genotypes .Results 16 specimens of amniotic fluid were non-se-creting type phenotype(30 .2% ) and 37 specimens of amniotic fluid were secreting type phenotype (69 .8% );48 specimens of amni-otic fluid were detected out the ABO blood group genotype by the PCR-SSP method .ABO blood group of fetal amniotic fluid cells by the gene identification was consistent to the detection results of amniotic fluid secreting type ABH blood group substances .Con-clusion The PCR-SSP technique can accurately detect the fetal amniotic fluid cells ABO blood group .%目的:通过检测羊水ABH血型物质和序列特异性引物-聚合酶链反应(PCR-SSP)基因技术检测胎儿羊水细胞ABO血型基因型,鉴定胎儿ABO血型。方法选取妊娠16~25周的孕妇53例,抽取羊水,利用间接凝集实验测定羊水AB H血型物质;将羊水细胞进行分离,提取羊水细胞DNA ,运用PCR-SSP技术分析其ABO血型基因型。结果16例羊水标本为非分泌型,占30.2%,37例羊水标本为分泌型,占69.8%;48例羊水标本通过PCR-SSP方法检测出了ABO血型的基因型。经基因鉴定的胎儿羊水细胞ABO血型与羊水分泌型ABH血型物质检测结果一致。结论 PCR-SSP技术可以准确地检测胎儿羊水细胞的ABO血型。

  7. Successful treatment of severe rhesus D-incompatible pregnancy with repeated double-filtration plasmapheresis.

    Science.gov (United States)

    Kamei, Koichi; Yamaguchi, Koushi; Sato, Mai; Ogura, Masao; Ito, Shuichi; Okada, Tomomi; Wada, Seiji; Sago, Haruhiko

    2015-10-01

    Fetal anemia is caused by Rhesus (RhD) sensitization as a result of RhD incompatibility during pregnancy. The severe form of this disease can cause hydrops fetalis leading to intrauterine death. We experienced a highly sensitized 39-year-old woman with B Rh-negative blood. She had a history of three induced abortions and experienced perinatal death associated with hydrops fetalis. During the pregnancy prior to her most recent one, she was treated with double-filtration plasmapheresis (DFPP), high dose γ-globulin and intrauterine fetal blood transfusion (IUT). For her most recent pregnancy, we performed only weekly or fortnightly DFPP from 13 weeks until delivery. Anti-D antibody titer was maintained between 32 and 256 without any signs of fetal anemia. IUT was not required at any stage of the pregnancy. No adverse events were observed. She successfully delivered a healthy male infant weighing 2,289 g by Cesarean section at 35 weeks. Repeated DFPP may be an effective and safe strategy to reduce antibody titers in highly sensitized women with RhD-incompatible pregnancy, avoiding the need for IUT. PMID:25413689

  8. Les mélanges de polymères incompatibles Incompatible Polymer Mixtures

    Directory of Open Access Journals (Sweden)

    Serpe G.

    2006-11-01

    Full Text Available Les mélanges de polymères thermoplastiques font actuellement l'objet d'un effort considérable de recherche et de développement. Cette synthèse bibliographique concerne principalement les mélanges mécaniques de polymères incompatibles à l'état fondu. Ceux-ci ont une morphologie hétérogène qui dépend des conditions de mise en oeuvre autant que de la nature des constituants. De plus, pour ce type de matériaux, la nature de l'interface joue un rôle déterminant sur la formation de la morphologie hétérogène et donc sur les propriétés du produit final. D'autre part un problème important réside dans la stabilité des mélanges réalisés. L'ajout d'une faible quantité d'agent tensioactif de type copolymère séquencé, ou greffé, ou la modification chimique des constituants (greffage ou réticulation par exemple permet, dans certains cas d'améliorer très sensiblement les propriétés des mélanges en augmentant l'adhérence entre les phases immiscibles. L'ensemble de ces considérations permettent la réalisation industrielle de nouveaux matériaux polymères aux caractéristiques spécifiques. Thermoplastic polymer mixtures are currently the subject of considerable research and development efforts. This bibliographic synthesis deals mainly with melt blending process of incompatible polymers. These blends have a heterogeneous morphology that depends on the processing conditions as much as on the chemical structure of the componants. Likewise, for these materials the nature of the interface plays a decisive role on the formation of the heterogeneous morphology and hence on the properties on the end product. Furthermore, an important problem is to stabilize the morphology of the blends. Adding a small amount of surfactant of the sequenced of grafted copolymer type or, else, the chemical reaction of the constituents (e. g. grafting or branching during processing are ways in some cases of considerably improving the properties of

  9. Blood cholesterol screening in several environments using a portable, dry-chemistry analyzer and fingerstick blood samples. Lipid Research Clinics Cholesterol Screening Study Group.

    Science.gov (United States)

    Bradford, R H; Bachorik, P S; Roberts, K; Williams, O D; Gotto, A M

    1990-01-01

    A multicenter study of blood cholesterol screening was performed in several typical environments, such as community sites (shopping malls and a supermarket), health care sites, work sites, a blood bank and a school. Cholesterol was measured with a portable, dry-chemistry analyzer using capillary blood obtained by fingerstick. Data are reported from a total of 13,824 participants, spanning the entire age spectrum. Overall, 25% of screened subjects had blood cholesterol levels above the age-specific cutpoints used in the current study. Although in the aggregate this screening experience very closely approximates the expected level of referrals, the proportion of referred screened subjects differed significantly among the 5 types of screening environments and by gender. Follow-up telephone interviews indicated that 53% of referrals had initiated a physician contact. More than 75% of those who had seen a physician reported that the diagnosis of hypercholesterolemia had been confirmed, and almost 72% had been prescribed a diet. A large proportion of referred screened subjects reported having modified their diet, particularly when recommended to do so by a physician. This study has yielded encouraging evidence that physicians gave referred screened subjects appropriate initial advice for managing hypercholesterolemia. The new technology for blood cholesterol measurement evaluated in the current study has proven to be a feasible and reliable means for measuring blood cholesterol in typical screening settings.

  10. Determinants of obesity among men with the lewis double-negative blood group: a genetic marker of obesity-the Copenhagen Male Study

    DEFF Research Database (Denmark)

    Gyntelberg, Finn; Hein, Hans Ole; Suadicani, Poul

    2011-01-01

    Middle-aged and elderly men with the Lewis blood group phenotype Le(a-b-), have a two-fold higher prevalence of obesity than others. We investigated if the association could be ascribed to differences in lifestyle, or if obesity determinants had a different impact in this group....

  11. Detention and incompatibility of HIV patients in Italy

    Directory of Open Access Journals (Sweden)

    Serena Dell'Isola

    2013-12-01

    Full Text Available In Italy, Law 231/99 and subsequent amendments standardize the conditions allowing or not a HIV positive inmate to remain in jail. Currently such clinical conditions are not automatically associated with the decline of preventive detention and the Court evaluates the incompatibility with detention on the basis of two additional and independent criteria. We have been observing the tendency by jailed HIV-positive patients to manipulate the disease state believing that the rules of incompatibility with the prison system are always applied. The management of HIV positive patients in jail involves significant sanitary and relational efforts, particularly for those suffering AIDS and/or with severe immunodeficiency.

  12. 南方鲇血型的初步鉴定%Preliminary Studies on the Blood Group of Southern Catfish Silurus meridionalis

    Institute of Scientific and Technical Information of China (English)

    黄林; 金丽; 张耀光

    2009-01-01

    To identify the blood group of the southern catfish Silurus meridionalis, cross-reactions were conducted between the fish's serum and red blood cells. The results showed that there was no agglutination in all the cross-reactions between the southern catfish's serum and individual red blood cells. This indicated that southern catfish may not have blood groups or that they may have blood groups but lack enough lectin in the serum. Using southern catfish red blood cells as the antigen to immune Japanese white rabbit to prepare antiserum, the prepared antiserum was used to cross-react with southern catfish red blood cells. The results showed that there were various degrees of agglutination which indicated the blood group existed in southern catfish. We could infer that southern catfish may have four blood groups which were named as N~A, N~B, N~(AB), N~O, and the method of preparing antiserum to cross-react with red blood cells is more reliable to identify the blood group of southern catfish.%本研究旨在通过观察南方鲇血清与其红细胞的交叉反应以鉴定南方鲇的血型.实验结果表明:南方鲇的血清与同种其他个体的红细胞进行交叉反应时均未出现凝集现象,这表明南方鲇可能不存在血型或南方鲇具备血型但血清中相应的凝集素含量不足.以南方鲇的红细胞为抗原免疫日本种大耳白兔制备的抗血清与南方鲇的红细胞进行交叉反应,出现了不同程度的凝集反应,这表明南方鲇存在血型.据上述两个实验结果可以推断,南方鲇可能存在4种血型,分别命名为N~A、N~B、N~(AB)和N~O型;同时也证实,在鉴定南方鲇血型的研究中,通过制备抗血清与红细胞进行交叉反应的方法更为可靠.

  13. SINGLE-CENTER EXPERIENCE OF ABO-INCOMPATIBLE LIVER TRANSPLANTATION

    Directory of Open Access Journals (Sweden)

    S. V. Gautier

    2011-01-01

    Full Text Available Since 2008 up to 2010 eight ABO-incompatible liver transplantations have been performed in our center: one of them was urgent liver transplantation to adult patient from deceased donor, other seven were transplantations of left lateral segment to children from living relative donors. Own experience, as well as world one, proves, that barrier of ABO-incompatibility can be overcome more successfully in liver transplantation, particularly in pediatric population, that in other solid organs transplantation. Good results can be achieved even with less ag- gressive immunosuppressive therapy. Recipient conditioning before operation can significantly improve results of ABO-incompatible liver transplantation, but as own experience has shown, often there’s no need to hold some special preparation of children, because their anti-ABO antibodies are very low or absent before transplantation and do not increase after it. Thereby ABO-incompatible liver transplantation is reasonable in urgent cases and in pediatric population because of the limited pull of living relative donors for children. 

  14. Genetic incompatibility drives mate choice in a parasitic wasp

    NARCIS (Netherlands)

    Thiel, Andra; Weeda, Anne C.; de Boer, Jetske G.; Hoffmeister, Thomas S.

    2013-01-01

    Introduction: Allelic incompatibility between individuals of the same species should select for mate choice based on the genetic make-up of both partners at loci that influence offspring fitness. As a consequence, mate choice may be an important driver of allelic diversity. A complementary sex deter

  15. Evolutionary dynamics of sporophytic self-incompatibility alleles in plants

    DEFF Research Database (Denmark)

    Schierup, M H; Vekemans, X; Christiansen, F B

    1997-01-01

    The stationary frequency distribution and allelic dynamics in finite populations are analyzed through stochastic simulations in three models of single-locus, multi-allelic sporophytic self-incompatibility. The models differ in the dominance relationships among alleles. In one model, alleles act c...

  16. Variance-based uncertainty relations for incompatible observables

    Science.gov (United States)

    Chen, Bin; Cao, Ning-Ping; Fei, Shao-Ming; Long, Gui-Lu

    2016-06-01

    We formulate uncertainty relations for arbitrary finite number of incompatible observables. Based on the sum of variances of the observables, both Heisenberg-type and Schrödinger-type uncertainty relations are provided. These new lower bounds are stronger in most of the cases than the ones derived from some existing inequalities. Detailed examples are presented.

  17. A simple genetic incompatibility causes hybrid male sterility in mimulus.

    Science.gov (United States)

    Sweigart, Andrea L; Fishman, Lila; Willis, John H

    2006-04-01

    Much evidence has shown that postzygotic reproductive isolation (hybrid inviability or sterility) evolves by the accumulation of interlocus incompatibilities between diverging populations. Although in theory only a single pair of incompatible loci is needed to isolate species, empirical work in Drosophila has revealed that hybrid fertility problems often are highly polygenic and complex. In this article we investigate the genetic basis of hybrid sterility between two closely related species of monkeyflower, Mimulus guttatus and M. nasutus. In striking contrast to Drosophila systems, we demonstrate that nearly complete hybrid male sterility in Mimulus results from a simple genetic incompatibility between a single pair of heterospecific loci. We have genetically mapped this sterility effect: the M. guttatus allele at the hybrid male sterility 1 (hms1) locus acts dominantly in combination with recessive M. nasutus alleles at the hybrid male sterility 2 (hms2) locus to cause nearly complete hybrid male sterility. In a preliminary screen to find additional small-effect male sterility factors, we identified one additional locus that also contributes to some of the variation in hybrid male fertility. Interestingly, hms1 and hms2 also cause a significant reduction in hybrid female fertility, suggesting that sex-specific hybrid defects might share a common genetic basis. This possibility is supported by our discovery that recombination is reduced dramatically in a cross involving a parent with the hms1-hms2 incompatibility.

  18. Consumption of lead-shot cervid meat and blood lead concentrations in a group of adult Norwegians.

    Science.gov (United States)

    Meltzer, H M; Dahl, H; Brantsæter, A L; Birgisdottir, B E; Knutsen, H K; Bernhoft, A; Oftedal, B; Lande, U S; Alexander, J; Haugen, M; Ydersbond, T A

    2013-11-01

    Several recent investigations have reported high concentrations of lead in samples of minced cervid meat. This paper describes findings from a Norwegian study performed in 2012 among 147 adults with a wide range of cervid game consumption. The main aim was to assess whether high consumption of lead-shot cervid meat is associated with increased concentration of lead in blood. A second aim was to investigate to what extent factors apart from game consumption explain observed variability in blood lead levels. Median (5 and 95 percentile) blood concentration of lead was 16.6 µg/L (7.5 and 39 µg/L). An optimal multivariate linear regression model for log-transformed blood lead indicated that cervid game meat consumption once a month or more was associated with approximately 31% increase in blood lead concentrations. The increase seemed to be mostly associated with consumption of minced cervid meat, particularly purchased minced meat. However, many participants with high and long-lasting game meat intake had low blood lead concentrations. Cervid meat together with number of bullet shots per year, years with game consumption, self-assembly of bullets, wine consumption and smoking jointly accounted for approximately 25% of the variation in blood lead concentrations, while age and sex accounted for 27% of the variance. Blood lead concentrations increased approximately 18% per decade of age, and men had on average 30% higher blood lead concentrations than women. Hunters who assembled their own ammunition had 52% higher blood lead concentrations than persons not making ammunition. In conjunction with minced cervid meat, wine intake was significantly associated with increased blood lead. Our results indicate that hunting practices such as use of lead-based ammunition, self-assembling of lead containing bullets and inclusion of lead-contaminated meat for mincing to a large extent determine the exposure to lead from cervid game consumption. PMID:24119336

  19. Effects of the combination of blood transfusion and postoperative infectious complications on prognosis after surgery for colorectal cancer. Danish RANX05 Colorectal Cancer Study Group

    DEFF Research Database (Denmark)

    Mynster, T; Christensen, Ib Jarle; Moesgaard, F;

    2000-01-01

    = 740) and time to diagnosis of recurrent disease in the subgroup of patients operated on with curative intention (n = 532). The patients were analysed in four groups divided with respect to administration or not of perioperative blood transfusion and development or non-development of postoperative......BACKGROUND: The frequency of postoperative infectious complications is significantly increased in patients with colorectal cancer receiving perioperative blood transfusion. It is still debated, however, whether perioperative blood transfusion alters the incidence of disease recurrence or otherwise...... affects the prognosis. METHODS: Patient risk variables, variables related to operation technique, blood transfusion and the development of infectious complications were recorded prospectively in 740 patients undergoing elective resection for primary colorectal cancer. Endpoints were overall survival (n...

  20. [Epidemiologic study of anti-HTLV-I/II antibodies in blood donors in metropolitan France. The Retrovirus Working Group of the National Blood Transfusion Society].

    Science.gov (United States)

    Lemaire, J M; Coste, J; Barin, F; Couroucé, A M

    1991-01-01

    Between march and april 1989, the prevalence of HTLV-I/II antibodies was studied in a non selected population of 45,033 blood donors of Continental France. Serum samples were collected and screened by 12 Blood Banks located in different parts of the country. Screening was performed by 4 ELISAs (Abbott-Du Pont de Nemours-Organon-Pharmacia) and by gelatin particle agglutination (Fujirebio); the sensitivity of these tests was previously evaluated with an HTLV-I/II panel of the French National Society of Transfusion. The reproducibly reactive sera were confirmed by Western-Blot (Du Pont) and radioimmunoprecipitation assay. Five donors were confirmed positive: the global prevalence is 0.011%. This prevalence is one of the highest in Europe. Three of the five positive donors (0.007%) are white subjects. The other two are natives from the French West Indies (0.81%). Since may 1989 the Ministry of Health recommends for Continental France to test donors originating from endemic zones or to use their plasma for fractionation. The results obtained in this study show that the efficacy of these measures are incomplete since 0.007% donors (1 out of 15,000 blood donations) are not tracked down.

  1. Wolbachia symbiont infections induce strong cytoplasmic incompatibility in the tsetse fly Glossina morsitans.

    Directory of Open Access Journals (Sweden)

    Uzma Alam

    2011-12-01

    Full Text Available Tsetse flies are vectors of the protozoan parasite African trypanosomes, which cause sleeping sickness disease in humans and nagana in livestock. Although there are no effective vaccines and efficacious drugs against this parasite, vector reduction methods have been successful in curbing the disease, especially for nagana. Potential vector control methods that do not involve use of chemicals is a genetic modification approach where flies engineered to be parasite resistant are allowed to replace their susceptible natural counterparts, and Sterile Insect technique (SIT where males sterilized by chemical means are released to suppress female fecundity. The success of genetic modification approaches requires identification of strong drive systems to spread the desirable traits and the efficacy of SIT can be enhanced by identification of natural mating incompatibility. One such drive mechanism results from the cytoplasmic incompatibility (CI phenomenon induced by the symbiont Wolbachia. CI can also be used to induce natural mating incompatibility between release males and natural populations. Although Wolbachia infections have been reported in tsetse, it has been a challenge to understand their functional biology as attempts to cure tsetse of Wolbachia infections by antibiotic treatment damages the obligate mutualistic symbiont (Wigglesworthia, without which the flies are sterile. Here, we developed aposymbiotic (symbiont-free and fertile tsetse lines by dietary provisioning of tetracycline supplemented blood meals with yeast extract, which rescues Wigglesworthia-induced sterility. Our results reveal that Wolbachia infections confer strong CI during embryogenesis in Wolbachia-free (Gmm(Apo females when mated with Wolbachia-infected (Gmm(Wt males. These results are the first demonstration of the biological significance of Wolbachia infections in tsetse. Furthermore, when incorporated into a mathematical model, our results confirm that Wolbachia can

  2. Wolbachia symbiont infections induce strong cytoplasmic incompatibility in the tsetse fly Glossina morsitans.

    Science.gov (United States)

    Alam, Uzma; Medlock, Jan; Brelsfoard, Corey; Pais, Roshan; Lohs, Claudia; Balmand, Séverine; Carnogursky, Jozef; Heddi, Abdelaziz; Takac, Peter; Galvani, Alison; Aksoy, Serap

    2011-12-01

    Tsetse flies are vectors of the protozoan parasite African trypanosomes, which cause sleeping sickness disease in humans and nagana in livestock. Although there are no effective vaccines and efficacious drugs against this parasite, vector reduction methods have been successful in curbing the disease, especially for nagana. Potential vector control methods that do not involve use of chemicals is a genetic modification approach where flies engineered to be parasite resistant are allowed to replace their susceptible natural counterparts, and Sterile Insect technique (SIT) where males sterilized by chemical means are released to suppress female fecundity. The success of genetic modification approaches requires identification of strong drive systems to spread the desirable traits and the efficacy of SIT can be enhanced by identification of natural mating incompatibility. One such drive mechanism results from the cytoplasmic incompatibility (CI) phenomenon induced by the symbiont Wolbachia. CI can also be used to induce natural mating incompatibility between release males and natural populations. Although Wolbachia infections have been reported in tsetse, it has been a challenge to understand their functional biology as attempts to cure tsetse of Wolbachia infections by antibiotic treatment damages the obligate mutualistic symbiont (Wigglesworthia), without which the flies are sterile. Here, we developed aposymbiotic (symbiont-free) and fertile tsetse lines by dietary provisioning of tetracycline supplemented blood meals with yeast extract, which rescues Wigglesworthia-induced sterility. Our results reveal that Wolbachia infections confer strong CI during embryogenesis in Wolbachia-free (Gmm(Apo)) females when mated with Wolbachia-infected (Gmm(Wt)) males. These results are the first demonstration of the biological significance of Wolbachia infections in tsetse. Furthermore, when incorporated into a mathematical model, our results confirm that Wolbachia can be used

  3. BibA: a novel immunogenic bacterial adhesin contributing to group B Streptococcus survival in human blood.

    Science.gov (United States)

    Santi, Isabella; Scarselli, Maria; Mariani, Massimo; Pezzicoli, Alfredo; Masignani, Vega; Taddei, Annarita; Grandi, Guido; Telford, John L; Soriani, Marco

    2007-02-01

    By the analysis of the recently sequenced genomes of Group B Streptococcus (GBS) we have identified a novel immunogenic adhesin with anti-phagocytic activity, named BibA. The bibA gene is present in 100% of the 24 GBS strains analysed. BibA-specific IgG were found in human sera from normal healthy donors. The putative protein product is a polypeptide of 630 amino acids containing a helix-rich N-terminal domain, a proline-rich region and a canonical LPXTG cell wall-anchoring domain. BibA is expressed on the surface of several GBS strains, but is also recovered in GBS culture supernatants. BibA specifically binds to human C4-binding protein, a regulator of the classic complement pathway. Deletion of the bibA gene severely reduced the capacity of GBS to survive in human blood and to resist opsonophagocytic killing by human neutrophils. In addition, BibA expression increased the virulence of GBS in a mouse infection model. The role of BibA in GBS adhesion was demonstrated by the impaired ability of a bibA knockout mutant strain to adhere to both human cervical and lung epithelial cells. Furthermore, we calculated that recombinant BibA bound to human epithelial cells of distinct origin with an affinity constant of approximately 10(-8) M for cervical epithelial cells. Hence BibA is a novel multifunctional protein involved in both resistance to phagocytic killing and adhesion to host cells. The identification of this potential new virulence factor represents an important step in the development of strategies to combat GBS-associated infections.

  4. Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane.

    Science.gov (United States)

    Amarasiri, Mohan; Hashiba, Satoshi; Miura, Takayuki; Nakagomi, Toyoko; Nakagomi, Osamu; Ishii, Satoshi; Okabe, Satoshi; Sano, Daisuke

    2016-05-15

    We demonstrated the genotype-dependent removal of human norovirus particles with a microfiltration (MF) membrane in the presence of bacteria bearing histo-blood group antigens (HBGAs). Three genotypes (GII.3, GII.4, and GII.6) of norovirus-like particles (NoVLPs) were mixed with three bacterial strains (Enterobacter sp. SENG-6, Escherichia coli O86:K61:B7, and Staphylococcus epidermidis), respectively, and the mixture was filtered with an MF membrane having a nominal pore size of 0.45 μm. All NoVLP genotypes were rejected by the MF membrane in the presence of Enterobacter sp. SENG-6, which excreted HBGAs as extracellular polymeric substances (EPS). This MF membrane removal of NoVLPs was not significant when EPS was removed from cells of Enterobacter sp. SENG-6. GII.6 NoVLP was not rejected with the MF membrane in the presence of E. coli O86:K61:B7, but the removal of EPS of E. coli O86:K61:B7 increased the removal efficiency due to the interaction of NoVLPs with the exposed B-antigen in lipopolysaccharide (LPS) of E. coli O86:K61:B7. No MF membrane removal of all three genotypes was observed when S. epidermidis, an HBGA-negative strain, was mixed with NoVLPs. These results demonstrate that the location of HBGAs on bacterial cells is an important factor in determining the genotype-dependent removal efficiency of norovirus particles with the MF membrane. The presence of HBGAs in mixed liquor suspended solids from a membrane bioreactor (MBR) pilot plant was confirmed by immune-transmission electron microscopy, which implies that bacterial HBGAs can contribute to the genotype-dependent removal of human noroviruses with MBR using MF membrane. PMID:27095709

  5. Methodology and technology for peripheral and central blood pressure and blood pressure variability measurement: current status and future directions - Position statement of the European Society of Hypertension Working Group on blood pressure monitoring and cardiovascular variability.

    Science.gov (United States)

    Stergiou, George S; Parati, Gianfranco; Vlachopoulos, Charalambos; Achimastos, Apostolos; Andreadis, Emanouel; Asmar, Roland; Avolio, Alberto; Benetos, Athanase; Bilo, Grzegorz; Boubouchairopoulou, Nadia; Boutouyrie, Pierre; Castiglioni, Paolo; de la Sierra, Alejandro; Dolan, Eamon; Head, Geoffrey; Imai, Yutaka; Kario, Kazuomi; Kollias, Anastasios; Kotsis, Vasilis; Manios, Efstathios; McManus, Richard; Mengden, Thomas; Mihailidou, Anastasia; Myers, Martin; Niiranen, Teemu; Ochoa, Juan Eugenio; Ohkubo, Takayoshi; Omboni, Stefano; Padfield, Paul; Palatini, Paolo; Papaioannou, Theodore; Protogerou, Athanasios; Redon, Josep; Verdecchia, Paolo; Wang, Jiguang; Zanchetti, Alberto; Mancia, Giuseppe; O'Brien, Eoin

    2016-09-01

    Office blood pressure measurement has been the basis for hypertension evaluation for almost a century. However, the evaluation of blood pressure out of the office using ambulatory or self-home monitoring is now strongly recommended for the accurate diagnosis in many, if not all, cases with suspected hypertension. Moreover, there is evidence that the variability of blood pressure might offer prognostic information that is independent of the average blood pressure level. Recently, advancement in technology has provided noninvasive evaluation of central (aortic) blood pressure, which might have attributes that are additive to the conventional brachial blood pressure measurement. This position statement, developed by international experts, deals with key research and practical issues in regard to peripheral blood pressure measurement (office, home, and ambulatory), blood pressure variability, and central blood pressure measurement. The objective is to present current achievements, identify gaps in knowledge and issues concerning clinical application, and present relevant research questions and directions to investigators and manufacturers for future research and development (primary goal). PMID:27214089

  6. An International Standard for specifying the minimum potency of anti-D blood-grouping reagents: evaluation of a candidate preparation in an international collaborative study

    NARCIS (Netherlands)

    S.J. Thorpe; B. Fox; A.B. Heath; M. Scott; M. de Haas; S. Kochman; A. Padilla

    2006-01-01

    Background and Objectives The aim of this study was to evaluate a lyophilized monoclonal immunoglobulin M (IgM) anti-D preparation for use as an International Standard to specify a recommended minimum acceptable potency of anti-D blood-grouping reagents. Materials and Methods The candidate Internati

  7. Genome Sequence of Enterobacter cloacae Strain SENG-6, a Bacterium Producing Histo-Blood Group Antigen-Like Substances That Can Bind with Human Noroviruses.

    Science.gov (United States)

    Ishii, Satoshi; Amarasiri, Mohan; Hashiba, Satoshi; Yang, Peiyi; Okabe, Satoshi; Sano, Daisuke

    2016-01-01

    Enterobacter sp. strain SENG-6, isolated from healthy human feces, produces histo-blood group antigen (HBGA)-like substances that can bind with human noroviruses. Based on the genome sequence analysis, strain SENG-6 belongs to the species Enterobacter cloacae The genome sequence of this strain should help identify genes associated with the production of HBGA-like substances.

  8. Human genetic polymorphisms in the Knops blood group are not associated with a protective advantage against Plasmodium falciparum malaria in Southern Ghana

    DEFF Research Database (Denmark)

    Hansson, Helle H; Kurtzhals, Jørgen A; Goka, Bamenla Q;

    2013-01-01

    The complex interactions between the human host and the Plasmodium falciparum parasite and the factors influencing severity of disease are still not fully understood. Human single nucleotide polymorphisms SNPs associated with Knops blood group system; carried by complement receptor 1 may...

  9. Premalignant and malignant oral lesions are associated with changes in the glycosylation pattern of carbohydrates related to ABH blood group antigens

    DEFF Research Database (Denmark)

    Dabelsteen, Erik; Clausen, H; Holmstrup, P;

    1988-01-01

    The distribution of carbohydrate structures related to the ABO(H) blood group antigen system was studied in biopsies from eight squamous cell carcinomas, and eight erythroplakias with epithelial dysplasia. Twenty oral lesions without histological evidence of malignancy (13 lichen planus lesions...

  10. Blood Groups in the Species Survival Plan®, European Endangered Species Program, and Managed in situ Populations of Bonobo (Pan paniscus), Common Chimpanzee (Pan troglodytes), Gorilla (Gorilla ssp.), and Orangutan (Pongo pygmaeus ssp.)

    OpenAIRE

    Gamble, Kathryn C.; Moyse, Jill A.; Lovstad, Jessica N.; Ober, Carole B.; Thompson, Emma E.

    2010-01-01

    Blood groups of humans and great apes long have been considered similar although are not interchangeable between species. In this study, human monoclonal antibody technology was used to assign human ABO blood groups to whole blood samples from great apes housed in North American and European zoos and in situ managed populations, as a practical means to assist blood transfusion situations for these species. From a subset of each of the species (bonobo, common chimpanzee, gorilla, and orangutan...

  11. Correlation bethealtyy ween dietary glycemic index and glycemic load and blood lipid levels in a group of women from Ahvaz

    OpenAIRE

    Farideh Shishebor; Zahra Shamekhi; Majid Karandish; Seyed Mahmood Latifi

    2011-01-01

    Background & Objectives: There are limited number of studies conducted on the correlation between Glycemic index and Glycemic load of a food program and metabolic factors such as blood lipids in Asian countries including Iran. Therefore, this study aimed at analyzing the correlation between Glycemic index and Glycemic load of Iranian food program and blood lipids. Materials & Methods: The subjects were 95 women working in Ahvaz University of Medical Sciences in the range of 20 to 55 years old...

  12. Cytomechanical properties of papaver pollen tubes are altered after self-incompatibility challenge

    NARCIS (Netherlands)

    Geitmann, A.; McConnaughey, W.; Lang-Pauluzzi, I.; Franklin-Tong, V.E.; Emons, A.M.C.

    2004-01-01

    Self-incompatibility (SI) in Papaver rhoeas triggers a ligand-mediated signal transduction cascade, resulting in the inhibition of incompatible pollen tube growth. Using a cytomechanical approach we have demonstrated that dramatic changes to the mechanical properties of incompatible pollen tubes are

  13. THE DISTRIBUTION OF ABO BLOOD GROUPS OF 3 434 MEDICAL STUDENTS%3434名医学生ABO血型分布调查

    Institute of Scientific and Technical Information of China (English)

    龙友国; 莫开勇; 江家志

    2011-01-01

    [Objective] To investigate the distributive feature of ABO blood groups of students in Qiannan Medical College for Nationalities.. [Methods] 3 434 students' ABO blood groups were identified by way of the slide agglutination. The data of ABO blood groups of students of 6 professional and 7 nations were analyzed. [ Results ] The distributive feature of ABO blood groups and gene frequency of A, B, O in all of 7 nations and 6 professional of students were O > A > B > AB and r > P> q respectively. The students in this medical college had a higher O genotype frequency. [Conclusion] The results suggest: (1) The distribution of ABO blood groups of students in this medical college has typical distributive features which the southerners have; (2) It may be correlated with their disposition.%[目的]调查贵州某医高专学生ABO血型分布特征.[方法]采用玻片凝集法检测 3434名医学生的ABO血型,对6个专业学生和人数大于50的民族的ABO血型数据进行统计分析.[结果]汉、布依、侗、苗、土家、彝和仡佬族等7个民族和6个专业学生的ABO血型分布特点为O>A>B>AB,基因频率分布特点是r>p>q.均具有较高的O型频率.[结论]结果提示:(1)该校学生ABO血型具有南方人的血型分布特征; (2)可能与他们的性格有一定关系.

  14. Covalent Fusion of layered Incompatible Gels in Immiscible Solvents

    Science.gov (United States)

    Biswas, Santidan; Singh, Awaneesh; Matyjaszewski, Krzysztof; Balazs, Anna C.

    We carry out dissipative particle dynamics (DPD) simulations to model a two layered stackable gel where the gels are incompatible and are present in immiscible solvent. The bottom layer of the gel is created first and then a solution of new initiators, monomers and cross-linkers is introduced on top of it. These components then undergo polymerization and form the second gel layer. We study all possible combinations of free radical polymerization (FRP) and atom transfer radical polymerization (ATRP) mechanisms with the two layers of the gel. For example, the bottom layer gel is created via ATRP, whereas the top layer gel follows FRP. Our focus is to do a systematic study of all these combinations and find out the factors responsible for combining two incompatible gels in immiscible solvents.

  15. 四川省汉族人群Duffy血型基因分型研究%A study on Duffy blood group genotyping of Han in Sichuan

    Institute of Scientific and Technical Information of China (English)

    蒿广德; 刘敏; 齐文玲; 蒲丽蓉; 赵素蓉; 刘铁梅

    2012-01-01

    目的 研究四川地区Duffy血型基因型分布,为建立红细胞库奠定基础.方法 对150名汉族健康献血者采用PCR-SSP方法进行Duffy血型系统进行基因分型检测.结果 四川地区汉族人群Duffy基因分型检测结果,Fya 基因频率为:0.929.Fyb:0.071;检出3例Fy(a-b-)个体.结论 PCR-SSP法检测Duffy血型简单、快捷、准确,为建立红细胞库奠定了基础.%Objective To study the distribution of Duffy blood group genotyping in Sichuan in China to establish the red cell bank. Methods PCR SSP was used to detect the Duffy blood group genotyping of 150 healthy blood donors. Results The genotyping frequency of Fya and Fyb wasO. 929 and 0. 071respectively in healthy blood donors, and 3 ca ses of Fy(a b )were found. Conclusion It is accurate and simple to use PCR SSP on detecting Duffy blood genotyping, and it is easier to detected to large scale investigation for red cell bank.

  16. Agglutinating mouse IgG3 compares favourably with IgMs in typing of the blood group B antigen: Functionality and stability studies.

    Science.gov (United States)

    Klaus, Tomasz; Bzowska, Monika; Kulesza, Małgorzata; Kabat, Agnieszka Martyna; Jemioła-Rzemińska, Małgorzata; Czaplicki, Dominik; Makuch, Krzysztof; Jucha, Jarosław; Karabasz, Alicja; Bereta, Joanna

    2016-01-01

    Mouse immunoglobulins M (IgMs) that recognize human blood group antigens induce haemagglutination and are used worldwide for diagnostic blood typing. Contrary to the current belief that IgGs are too small to simultaneously bind antigens on two different erythrocytes, we obtained agglutinating mouse IgG3 that recognized antigen B of the human ABO blood group system. Mouse IgG3 is an intriguing isotype that has the ability to form Fc-dependent oligomers. However, F(ab')2 fragments of the IgG3 were sufficient to agglutinate type B red blood cells; therefore, IgG3-triggered agglutination did not require oligomerization. Molecular modelling indicated that mouse IgG3 has a larger range of Fab arms than other mouse IgG subclasses and that the unique properties of mouse IgG3 are likely due to the structure of its hinge region. With a focus on applications in diagnostics, we compared the stability of IgG3 and two IgMs in formulated blood typing reagents using an accelerated storage approach and differential scanning calorimetry. IgG3 was much more stable than IgMs. Interestingly, the rapid decrease in IgM activity was caused by aggregation of the molecules and a previously unknown posttranslational proteolytic processing of the μ heavy chain. Our data point to mouse IgG3 as a potent diagnostic tool. PMID:27484487

  17. Fine specificities of two lectins from Cymbosema roseum seeds: a lectin specific for high-mannose oligosaccharides and a lectin specific for blood group H type II trisaccharide.

    Science.gov (United States)

    Dam, Tarun K; Cavada, Benildo S; Nagano, Celso S; Rocha, Bruno Am; Benevides, Raquel G; Nascimento, Kyria S; de Sousa, Luiz Ag; Oscarson, Stefan; Brewer, C Fred

    2011-07-01

    The legume species of Cymbosema roseum of Diocleinae subtribe produce at least two different seed lectins. The present study demonstrates that C. roseum lectin I (CRL I) binds with high affinity to the "core" trimannoside of N-linked oligosaccharides. Cymbosema roseum lectin II (CRL II), on the other hand, binds with high affinity to the blood group H trisaccharide (Fucα1,2Galα1-4GlcNAc-). Thermodynamic and hemagglutination inhibition studies reveal the fine binding specificities of the two lectins. Data obtained with a complete set of monodeoxy analogs of the core trimannoside indicate that CRL I recognizes the 3-, 4- and 6-hydroxyl groups of the α(1,6) Man residue, the 3- and 4-hydroxyl group of the α(1,3) Man residue and the 2- and 4-hydroxyl groups of the central Man residue of the trimannoside. CRL I possesses enhanced affinities for the Man5 oligomannose glycan and a biantennary complex glycan as well as glycoproteins containing high-mannose glycans. On the other hand, CRL II distinguishes the blood group H type II epitope from the Lewis(x), Lewis(y), Lewis(a) and Lewis(b) epitopes. CRL II also distinguishes between blood group H type II and type I trisaccharides. CRL I and CRL II, respectively, possess differences in fine specificities when compared with other reported mannose and fucose recognizing lectins. This is the first report of a mannose-specific lectin (CRL I) and a blood group H type II-specific lectin (CRL II) from seeds of a member of the Diocleinae subtribe.

  18. On the Evolution of Genetic Incompatibility Systems. VI. a Three-Locus Modifier Model for the Origin of Gametophytic Self-Incompatibility

    OpenAIRE

    Uyenoyama, M.K.

    1991-01-01

    Recent genetic analyses have demonstrated that self-incompatibility in flowering plants derives from the coordinated expression of a system of loci. To address the selective mechanisms through which a genetic system of this kind evolves, I present a three-locus model for the origin of gametophytic self-incompatibility. Conventional models assume that a single locus encodes all physiological effects associated with self-incompatibility and that the viability of offspring depends only on whethe...

  19. Identification of incompatibility alleles in the tetraploid species sour cherry.

    Science.gov (United States)

    Tobutt, K R; Bosković, R; Cerović, R; Sonneveld, T; Ruzić, D

    2004-03-01

    The incompatibility genetics of sour cherry ( Prunus cerasus), an allotetraploid species thought to be derived from sweet cherry (diploid) and ground cherry (tetraploid), were investigated by test crossing and by analysis of stylar ribonucleases which are known to be the products of incompatibility alleles in sweet cherry. Stylar extracts of 36 accessions of sour cherry were separated electrophoretically and stained for ribonuclease activity. The zymograms of most accessions showed three bands, some two or four. Of the ten bands seen, six co-migrated with bands that in sweet cherry are attributed to the incompatibility alleles S(1), S(3), S(4), S(6, ) S(9) and S(13). 'Cacanski Rubin', 'Erdi Botermo B', 'Koros' and 'Ujfehertoi Furtos', which showed bands apparently corresponding to S(1) and S(4), were test pollinated with the sweet cherry 'Merton Late' ( S(1) S(4)). Monitoring pollen tube growth, and, in one case, fruit set, showed that these crosses were incompatible and that the four sour cherries indeed have the alleles S(1) and S(4). Likewise, test pollination of 'Marasca Piemonte', 'Marasca Savena' and 'Morello, Dutch' with 'Noble' ( S(6) S(13)) showed that these three sour cherries have the alleles S(6) and S(13). S(13) was very frequent in sour cherry cultivars, but is rare in sweet cherry cultivars, whereas with S(3) the situation is reversed. It was suggested that the other four bands are derived from ground cherry and one of these, provisionally attributed to S(B), occurred frequently in a small set of ground cherry accessions surveyed. Analysing some progenies from sour by sweet crosses by S allele-specific PCR and monitoring the success of some sweet by sour crosses were informative. They indicated mostly disomic inheritance, with sweet cherry S alleles belonging to one locus and, presumably, the ground cherry alleles to the other, and helped clarify the genomic arrangement of the alleles and the interactions in heteroallelic pollen. PMID:14689184

  20. Identification of Chrysanthemum (Chrysanthemum morifolium Self-Incompatibility

    Directory of Open Access Journals (Sweden)

    Fan Wang

    2014-01-01

    Full Text Available There has been a heated argument over self-incompatibilityof chrysanthemum (Chrysanthemum morifolium among chrysanthemum breeders. In order to solve the argument, we investigated pistil receptivity, seed set, and compatible index of 24 chrysanthemum cultivars. It was found that the 24 cultivars averagely had 3.7–36.3 pollen grains germinating on stigmas at 24 hours after self-pollination through the fluorescence microscope using aniline blue staining method. However, only 10 of them produced self-pollinated seeds, and their seed sets and compatible indexes were 0.03–56.50% and 0.04–87.50, respectively. The cultivar “Q10-33-1” had the highest seed set (56.50% and compatible index (87.50, but ten of its progeny had a wide range of separation in seed set (0–37.23% and compatible index (0–68.65. The results indicated that most of chrysanthemum cultivars were self-incompatible, while a small proportion of cultivars were self-compatible. In addition, there is a comprehensive separation of self-incompatibility among progeny from the same self-pollinated self-compatible chrysanthemum cultivar. Therefore, it is better to emasculate inflorescences during chrysanthemum hybridization breeding when no information concerning its self-incompatibility characteristics is available. However, if it is self-incompatible and propagated by vegetative methods, it is unnecessary to carry out emasculation when it is used as a female plant during hybridization breeding.

  1. Distyly and heteromorphic self-incompatibility of Hedyotis pulcherrima (Rubiaceae)

    OpenAIRE

    Xinxin Liu; Xiaoqin Wu; Dianxiang Zhang

    2012-01-01

    Heterostyly is a genetically controlled floral polymorphism, which includes both distyly and tristyly. We investigated morph ratio, floral and pollen morphology, and self-incompatibility of Hedyotispulcherrima. Overall, the natural population of H. pulcherrima was isoplethic, containing long-styled and short-styled morphs with an equilibrium 1:1 ratio. Long-styled and short-styled morph exhibited a precise reciprocal herkogamy, which was significantly correlated with corolla length. Stigma-lo...

  2. The different mechanisms of sporophytic self-incompatibility.

    OpenAIRE

    Hiscock, Simon J; Tabah, David A

    2003-01-01

    Flowering plants have evolved a multitude of mechanisms to avoid self-fertilization and promote outbreeding. Self-incompatibility (SI) is by far the most common of these, and is found in ca. 60% of flowering plants. SI is a genetically controlled pollen-pistil recognition system that provides a barrier to fertilization by self and self-related pollen in hermaphrodite (usually co-sexual) flowering plants. Two genetically distinct forms of SI can be recognized: gametophytic SI (GSI) and sporoph...

  3. Selfish evolution of cytonuclear hybrid incompatibility in Mimulus.

    Science.gov (United States)

    Case, Andrea L; Finseth, Findley R; Barr, Camille M; Fishman, Lila

    2016-09-14

    Intraspecific coevolution between selfish elements and suppressors may promote interspecific hybrid incompatibility, but evidence of this process is rare. Here, we use genomic data to test alternative models for the evolution of cytonuclear hybrid male sterility in Mimulus In hybrids between Iron Mountain (IM) Mimulus guttatus × Mimulus nasutus, two tightly linked M. guttatus alleles (Rf1/Rf2) each restore male fertility by suppressing a local mitochondrial male-sterility gene (IM-CMS). Unlike neutral models for the evolution of hybrid incompatibility loci, selfish evolution predicts that the Rf alleles experienced strong selection in the presence of IM-CMS. Using whole-genome sequences, we compared patterns of population-genetic variation in Rf at IM to a neighbouring population that lacks IM-CMS. Consistent with local selection in the presence of IM-CMS, the Rf region shows elevated FST, high local linkage disequilibrium and a distinct haplotype structure at IM, but not at Cone Peak (CP), suggesting a recent sweep in the presence of IM-CMS. In both populations, Rf2 exhibited lower polymorphism than other regions, but the low-diversity outliers were different between CP and IM. Our results confirm theoretical predictions of ubiquitous cytonuclear conflict in plants and provide a population-genetic mechanism for the evolution of a common form of hybrid incompatibility. PMID:27629037

  4. Validation of cytogenetic risk groups according to International Prognostic Scoring Systems by peripheral blood CD34+FISH: results from a German diagnostic study in comparison with an international control group

    Science.gov (United States)

    Braulke, Friederike; Platzbecker, Uwe; Müller-Thomas, Catharina; Götze, Katharina; Germing, Ulrich; Brümmendorf, Tim H.; Nolte, Florian; Hofmann, Wolf-Karsten; Giagounidis, Aristoteles A. N.; Lübbert, Michael; Greenberg, Peter L.; Bennett, John M.; Solé, Francesc; Mallo, Mar; Slovak, Marilyn L.; Ohyashiki, Kazuma; Le Beau, Michelle M.; Tüchler, Heinz; Pfeilstöcker, Michael; Nösslinger, Thomas; Hildebrandt, Barbara; Shirneshan, Katayoon; Aul, Carlo; Stauder, Reinhard; Sperr, Wolfgang R.; Valent, Peter; Fonatsch, Christa; Trümper, Lorenz; Haase, Detlef; Schanz, Julie

    2015-01-01

    International Prognostic Scoring Systems are used to determine the individual risk profile of myelodysplastic syndrome patients. For the assessment of International Prognostic Scoring Systems, an adequate chromosome banding analysis of the bone marrow is essential. Cytogenetic information is not available for a substantial number of patients (5%–20%) with dry marrow or an insufficient number of metaphase cells. For these patients, a valid risk classification is impossible. In the study presented here, the International Prognostic Scoring Systems were validated based on fluorescence in situ hybridization analyses using extended probe panels applied to cluster of differentiation 34 positive (CD34+) peripheral blood cells of 328 MDS patients of our prospective multicenter German diagnostic study and compared to chromosome banding results of 2902 previously published patients with myelodysplastic syndromes. For cytogenetic risk classification by fluorescence in situ hybridization analyses of CD34+ peripheral blood cells, the groups differed significantly for overall and leukemia-free survival by uni- and multivariate analyses without discrepancies between treated and untreated patients. Including cytogenetic data of fluorescence in situ hybridization analyses of peripheral CD34+ blood cells (instead of bone marrow banding analysis) into the complete International Prognostic Scoring System assessment, the prognostic risk groups separated significantly for overall and leukemia-free survival. Our data show that a reliable stratification to the risk groups of the International Prognostic Scoring Systems is possible from peripheral blood in patients with missing chromosome banding analysis by using a comprehensive probe panel (clinicaltrials.gov identifier:01355913). PMID:25344522

  5. Can a Single Session of a Community-Based Group Exercise Program Combining Step Aerobics and Bodyweight Resistance Exercise Acutely Reduce Blood Pressure?

    OpenAIRE

    Mendes Romeu; Sousa Nelson; Garrido Nuno; Cavaco Braulio; Quaresma Luís; Reis Victor Machado

    2014-01-01

    This study aimed to analyze the acute effects of a single session of a community-based group exercise program combining step aerobics and bodyweight resistance exercise on blood pressure in healthy young adult women. Twenty-three healthy young adult women (aged 31.57 ± 7.87 years) participated in two experimental sessions (exercise and control) in a crossover study design. Blood pressure was monitored before, immediately after and at 10, 20 and 30 min of recovery. The exercise session consist...

  6. Ethico-legal aspects of hospital-based blood transfusion practice; implications of professional negligence to medical practitioners: a review

    Directory of Open Access Journals (Sweden)

    Orkuma J.A

    2014-12-01

    Full Text Available Background: Blood transfusion is predominantly a hospital-based practice in many resourceconstrained economies like Nigeria, wherein the sourcing, storage, processing and clinical use of blood and blood products resides in the often financial and manpower constrained hospitals. Aim: To identify the ethical and legal issues related to hospital-based blood transfusion practice for medical practitioner. Methods: Relevant articles retrieved via PubMed/MEDLINE and Google scholar search engines were used. Results: This review found that, medical practitioners are directly or vicariously liable in professional negligence in hospital-based transfusion injuries. The potential weaknesses in hospital-based blood transfusion practice as ethically identified include; transfusion transmissible infections, blood group incompatibility, haemolytic transfusion reaction, failure to obtain informed consent and challenges of haemovigillance. However from the cases considered, medical practitioners are more professionally liable where they withhold a transfusion or transfuse blood group incompatible transfusion or fail to obtain an informed consent for blood transfusion especially amongst Jehovah’s Witness adherents. Conclusion: Medical practitioners at hospital-based transfusion centres are professionally liable for negligence due to blood transfusion injuries by their clinical, laboratory or general administrative oversight at different levels of hospital management. It is recommended that, they carry out this duty cautiously. While error reporting should be encouraged, litigations against them should be pursued cautiously in order not to encourage defensive blood transfusion practices in fear of claims or litigations to the disadvantage of the patients. Mediation or arbitration systems which are faster, less expensive and often less punitive but help strengthen the blood transfusion services should be encouraged.

  7. Current trends in platelet transfusions practice: The role of ABO-RhD and human leukocyte antigen incompatibility

    Directory of Open Access Journals (Sweden)

    Serena Valsami

    2015-01-01

    Full Text Available Platelet transfusions have contributed to the revolutionary modern treatment of hypoproliferative thrombocytopenia. Despite the long-term application of platelet transfusion in therapeutics, all aspects of their optimal use (i.e., in cases of ABO and/or Rh (D incompatibility have not been definitively determined yet. We reviewed the available data on transfusion practices and outcome in ABO and RhD incompatibility and platelet refractoriness due to anti-human leukocyte antigen (HLA antibodies. Transfusion of platelets with major ABO-incompatibility is related to reduced posttransfusion platelet (PLT count increments, compared to ABO-identical and minor, but still are equally effective in preventing clinical bleeding. ABO-minor incompatible transfusions pose the risk of an acute hemolytic reaction of the recipient that is not always related to high anti-A, B donor titers. ABO-identical PLT transfusion seems to be the most effective and safest therapeutic strategy. Exclusive ABO-identical platelet transfusion policy could be feasible, but alternative approaches could facilitate platelet inventory management. Transfusion of platelets from RhD positive donors to RhD negative patients is considered to be effective and safe though is associated with low rate of anti-D alloimmunization due to contaminating red blood cells. The prevention of D alloimmunization is recommended only for women of childbearing age. HLA alloimmunization is a major cause of platelet refractoriness. Managing patients with refractoriness with cross-matched or HLA-matched platelets is the current practice although data are still lacking for the efficacy of this practice in terms of clinical outcome. Leukoreduction contributes to the reduction of both HLA and anti-D alloimmunization.

  8. Current trends in platelet transfusions practice: The role of ABO-RhD and human leukocyte antigen incompatibility.

    Science.gov (United States)

    Valsami, Serena; Dimitroulis, Dimitrios; Gialeraki, Argyri; Chimonidou, Maria; Politou, Marianna

    2015-01-01

    Platelet transfusions have contributed to the revolutionary modern treatment of hypoproliferative thrombocytopenia. Despite the long-term application of platelet transfusion in therapeutics, all aspects of their optimal use (i.e., in cases of ABO and/or Rh (D incompatibility) have not been definitively determined yet. We reviewed the available data on transfusion practices and outcome in ABO and RhD incompatibility and platelet refractoriness due to anti-human leukocyte antigen (HLA) antibodies. Transfusion of platelets with major ABO-incompatibility is related to reduced posttransfusion platelet (PLT) count increments, compared to ABO-identical and minor, but still are equally effective in preventing clinical bleeding. ABO-minor incompatible transfusions pose the risk of an acute hemolytic reaction of the recipient that is not always related to high anti-A, B donor titers. ABO-identical PLT transfusion seems to be the most effective and safest therapeutic strategy. Exclusive ABO-identical platelet transfusion policy could be feasible, but alternative approaches could facilitate platelet inventory management. Transfusion of platelets from RhD positive donors to RhD negative patients is considered to be effective and safe though is associated with low rate of anti-D alloimmunization due to contaminating red blood cells. The prevention of D alloimmunization is recommended only for women of childbearing age. HLA alloimmunization is a major cause of platelet refractoriness. Managing patients with refractoriness with cross-matched or HLA-matched platelets is the current practice although data are still lacking for the efficacy of this practice in terms of clinical outcome. Leukoreduction contributes to the reduction of both HLA and anti-D alloimmunization. PMID:26420927

  9. Funções biológicas dos antígenos eritrocitários Biological functions of blood group antigens

    Directory of Open Access Journals (Sweden)

    Silvia L. Bonifácio

    2009-04-01

    Full Text Available Os antígenos de grupos sanguíneos eritrocitários são estruturas macromoleculares localizadas na superfície extracelular da membrana eritrocitária. Com o desenvolvimento de estudos moleculares, mais de 250 antígenos são conhecidos e estão organizados em 29 sistemas de grupos sanguíneos reconhecidos pela Sociedade Internacional de Transfusão Sanguínea (ISBT. Estudos têm revelado que os antígenos de grupo sanguíneo estão expressos na membrana eritrocitária com ampla diversidade estrutural, incluindo epítopos de carboidratos em glicoproteínas e/ou glicolipídios e em proteínas inseridas na membrana via um domínio, via domínios de multipassagem ou ligados a glicosilfosfatidinositol. Além das diversidades estruturais, muitas funções importantes têm sido associadas aos antígenos eritrocitários recentemente identificadas, podendo ser esquematicamente divididas em: estruturais, transportadores, receptores e moléculas de adesão, enzimas, proteínas controladoras do complemento e outras. Esta revisão tem como foco as funções potenciais das moléculas que expressam os antígenos eritrocitários.Erythrocyte blood group antigens are macromolecules structures located on the extracellular surface of the red blood cell membrane. The development of molecular studies allowed the recognition of more than 250 antigens by the International Society for Blood Transfusion (ISBT. These studies have also shown that blood group antigens are carried on red blood cell membrane of wide structural diversity, including carbohydrate epitopes on glycoproteins and/or glycolipids and on proteins inserted within the membrane via single or multi-pass transmembrane domains, or via glycosylphosphatidylinositol linkages. In addition, to their structural diversity, many important functions associated with blood group antigens have been recently identified and can be didactically divided into: structural proteins, transporters, receptors and adhesion

  10. Qualitative and Quantitative Analysis of the Binding of GII.4 Norovirus Variants onto Human Blood Group Antigens▿

    Science.gov (United States)

    de Rougemont, A.; Ruvoen-Clouet, N.; Simon, B.; Estienney, M.; Elie-Caille, C.; Aho, S.; Pothier, P.; Le Pendu, J.; Boireau, W.; Belliot, G.

    2011-01-01

    Noroviruses (NoVs) are one of the leading causes of gastroenteritis in children and adults. For the last 2 decades, genogroup II genotype 4 (GII.4) NoVs have been circulating worldwide. GII.4 NoVs can be divided into variants, and since 2002 they have circulated in the population before being replaced every 2 or 3 years, which raises questions about the role of their histo-blood group antigen (HBGA) ligands in their evolution. To shed light on these questions, we performed an analysis of the interaction between representative GII.4 variants and HBGAs, and we determined the role of selected amino acids in the binding profiles. By mutagenesis, we showed that there was a strict structural requirement for the amino acids, directly implicated in interactions with HBGAs. However, the ablation of the threonine residue at position 395 (ΔT395), an epidemiological feature of the post-2002 variants, was not deleterious to the binding of the virus-like particle (VLP) to the H antigen, while binding to A and B antigens was severely hampered. Nevertheless, the ΔT395 VLPs gained the capacity to bind to the Lewis x and sialyl-Lewis x antigens in comparison with the wild-type VLP, demonstrating that amino acid residues outside the HBGA binding site can modify the binding properties of NoVs. We also analyzed the attachment of baculovirus-expressed VLPs from six variants (Bristol, US95/96, Hunter, Yerseke, Den Haag, and Osaka) that were isolated from 1987 to 2007 to phenotyped saliva samples and synthetic HBGAs. We showed that the six variants could all attach to saliva of secretors irrespective of the ABO phenotype and to oligosaccharides characteristic of the secretor phenotype. Interestingly, Den Haag and Osaka variants additionally bound to carbohydrates present in the saliva of Lewis-positive nonsecretors. The carbohydrate binding profile and the genetic and mutagenesis analysis suggested that GII.4 binding to Lewis x and sialyl-Lewis x antigens might be a by-product of the

  11. 96例 Rh 血型抗体检测及分析%Detection and analysis on 96 cases of Rh blood group antibody

    Institute of Scientific and Technical Information of China (English)

    方晓蕾; 梅礼军; 刘锋; 张杰; 禹梅; 陈蕾

    2015-01-01

    Objective To analyze the positive rate of specific distribution characteristics in Rh blood group antibody,analyze the clinical significance of Rh blood group antibody and rules.Methods The micro column gel anti globulin technique was used to screen and identify irregular red blood cell antibodies,for patients with Rh blood group antibody,monoclonal anti-D,anti-C,anti-c, anti-E,anti-e were used to identify Rh blood group antigen to confirm the accuracy of detection.The antibody titer,Ig-type and 37℃ reactive were used to determine its clinical significance.Through asking pregnancy history,history of blood transfusion,under-standing whether the same specificity of the antibody in maternal plasma if the patient was newborn,the causes of antibody were an-alyzed.Results In 109 000 patients,Rh blood group antibodies were detected in 96 cases,the positive rate was 0.088%,which has a history of pregnancy in 68 cases,5 cases had history of blood transfusion,both pregnancy history and history of blood transfusion in 6 cases,1 7 cases of neonatal maternally derived antibody.Antibody specificity:65 cases of anti-E(67.710%),12 cases of anti-cE (12.500%),8 cases of anti-D (8.330%),7 cases of anti-c(7.291%),2 cases of anti-C (2.083%),2 cases of anti-e(2.083%).96 cases of Rh blood group antibodies were IgG or IgG+IgM class,37 ℃ reaction could be with the corresponding antigen of red blood cell,antibody titer between 4-2 048.Conclusion Anti-D detection rate shows a trend of gradually decreasing.In Rh blood group antibody detection,anti-E and anti-cE account for an absolute majority.Alloimmune caused by pregnancies and blood transfusion is the main reason of Rh blood group antibody production from Rh blood group antibody.Neonatal maternal passive getisa Rh-HDN is the main pathogenic antibody.%目的:调查 Rh血型抗体的检出率及其特异性分布特点,分析 Rh血型抗体的临床意义及产生规律。方法采用微柱凝胶抗球蛋白技术筛查和鉴定红细

  12. [Genetic polymorphism of blood group and erythrocyte enzymes in three ethno-territorial groups of the northern European part of Russia].

    Science.gov (United States)

    Evseeva, I V; Spitsyn, V A; Makarov, S V; Bychkovskaia, L S; Paé, G V

    2001-11-01

    Using the data on five red cell markers (AB0, PGM1, ACP1, GLO1, and ESD) polymorphisms, the population genetic structure of three ethnic territorial groups from the north of European Russia (Continental Nentsy, Kola Saami, and Russian Coast-dwellers) was described. In general, the groups studied a Caucasoid pattern of the frequency distribution of erythrocytic marker alleles. However, a substantial contribution of a Mongoloid component to the Nenets gene pool, expressed as a high frequency of the PGM1*1 allele along with a low frequency of the GLO1*1 allele, was observed. Three ethnic territorial groups examined were close to one another with respect to the distribution of classical biochemical markers. The interpopulation diversity was low (the mean FST = 0.015). The differences observed were for the most part caused by the genetic characteristics of Nentsy. The maximum interpopulation diversity was observed for the GLO1 locus (FST = 0.056).

  13. The non-Mendelian inheritance of Lewis-c blood group substance, as demonstrated in the case of a Bombay, Le(a-b-c-) saliva.

    Science.gov (United States)

    Savvas, R S

    1975-01-01

    A Bombay, Le(a-b-) saliva was shown to lack Pneumococcus type XIV activity, an unusual situation, since this sample should be rich in this precursor to the ABO blood group substances. However, the sample was found to contain a new serological specificity, Le-c. It is argued that simple Mendelian inheritance does not occur with Le-c and single gene control cannot be demonstrated. Failure to repress a fetal gene at birth, as implicated by the similarity in structure between Le-c and carcinoembryonic antigen [SIMMONS and PERLMANN], has been excluded as the mechanism of inheritance of this blood group substance, due to the inability to detect carcinoembryonic antigen in the test saliva.

  14. Genetic admixture, social-behavioral factors, and body composition are associated with blood pressure differently by racial-ethnic group among children.

    OpenAIRE

    Klimentidis, Yann C.; Dulin-Keita, Akilah; Casazza, Krista; Willig, Amanda L.; Allison, David B.; Fernandez, Jose R.

    2011-01-01

    Cardiovascular disease has a progressively earlier age of onset, and disproportionately affects African Americans in the US. It has been difficult to establish the extent to which group differences are due to physiological, genetic, social, or behavioral factors. In this study, we examined the association between blood pressure and these factors among a sample of 294 children, identified as African-, European-, or Hispanic-American. We use body composition, behavioral (diet and physical activ...

  15. The A0 blood group genotype modifies the jejunal glycomic binding pattern profile of piglets early associated with a simple or complex microbiota.

    Science.gov (United States)

    Priori, D; Colombo, M; Koopmans, S-J; Jansman, A J M; van der Meulen, J; Trevisi, P; Bosi, P

    2016-02-01

    The intestinal epithelium glycocalyx sugar motif is an important determinant of the bacterial-host interaction and may be affected in pigs by gut microbiota and by blood group genotype. The aim was to study the effect of intestinal association with different microbiota and A0 blood group genotypes on the expressed glycomic pattern in the small intestine. Twelve caesarean-derived pigs previously associated with a simple association (SA) or complex association (CA) microbiota were selected at 26 to 37 d of age. In each subject, different jejunal loops were perfused for 8 h with enterotoxigenic K88 (ETEC), ETEC fimbriae (F4), (LAM), or a saline control. The piglets were genotyped for A0 blood group and the glycomic profile was evaluated by microscopic screening of lectin binding: peanut agglutinin (PNA), which is galactose specific; agglutinin I (UEA), which is fucose specific; lectin II (MALii), which is sialic acid specific; concavalin A, which is mannose specific; soybean agglutinin (SBA), which is -acetyl-galactosamine specific; and wheat germ agglutinin (WGA), which is -acetyl-glucosamine specific. A0 pigs had fewer UEA-positive cells, MALii-positive cells ( < 0.001), and SBA-positive cells ( < 0.10) than 00 pigs. Simple association pigs had more SBA positive cells ( < 0.01) than CA pigs. Enterotoxigenic K88-perfused intestinal loops had fewer UEA-positive cells ( < 0.01) and WGA positive cells ( < 0.001) cells and more PNA positive cells (only in SA pigs, < 0.01). No effects of introduction of F4 and LAM in the intestinal lumen were observed. The porcine A0 blood group genotype and the luminal presence of ETEC strongly affected the jejunal mucosa glycomic pattern profile whereas an early oral simple or complex microbial association had limited effects. Pig genetic background has relevance on the cross talk between intestinal epithelium glycocalyx sugar motif and ETEC and, ultimately, on the gut microbial colonization in later life. PMID:27065129

  16. In vitro activities of eight macrolide antibiotics and RP-59500 (quinupristin-dalfopristin) against viridans group streptococci isolated from blood of neutropenic cancer patients.

    OpenAIRE

    Alcaide, F; Carratala, J.; Liñares, J; Gudiol, F; R. Martin

    1996-01-01

    From January 1988 to December 1994, 66 consecutive blood culture isolates of viridans group streptococci collected from febrile neutropenic cancer patients were tested for antimicrobial susceptibilities by the agar dilution method. The antibiotics studied were erythromycin, clarithromycin, roxithromycin, dirithromycin, azithromycin, josamycin, diacetyl-midecamycin, spiramycin, and quinupristin-dalfopristin. A total of 26 (39.4%) strains were resistant to erythromycin with an MIC range of 0.5 ...

  17. Effect of Group Mindfulness-Based Stress-Reduction Program and Conscious Yoga on Lifestyle, Coping Strategies, and Systolic and Diastolic Blood Pressures in Patients with Hypertension

    Directory of Open Access Journals (Sweden)

    Somayeh Nejati

    2015-12-01

    Full Text Available Background: Healthy lifestyle and ineffective coping strategies are deemed significant variables among patients with hypertension. This study attempted to determine the status of these variables following intervention via the mindfulness-based stress-reduction program (MBSRP in patients with hypertension.Method: This study was a randomized clinical trial. The study sample, consisting of 30 patients referring to the Hypertension Clinic of Imam Hossein Hospital in 2013, was assigned either to the intervention (recipient of the MBSRP and conscious yoga or to the control group (recipient of yoga training. The intervention group had 8 training sessions over 8 weeks. Lifestyle and coping strategies as well as blood pressure were measured in the intervention group before intervention and then immediately thereafter and at 2 months' follow-up and were compared to those in the control group at the same time points.Result: The mean age of the patients in the intervention (40% women and control (53% women groups was 43.66 ± 5.14 and 43.13 ± 5.04 years, respectively. The results showed that the mean scores of lifestyle (p value < 0.05, emotion-focused coping strategies (p value < 0.001, problem-focused coping strategies (p value < 0.001, diastolic blood pressure (p value < 0.001, and systolic blood pressure (p value < 0.001 were significantly different between the intervention and control groups after the intervention.Conclusion: Applying an intervention based on the MBSRP may further improve the lifestyle and coping strategies of patients with hypertension.

  18. Increased risk of venous thrombosis by AB alleles of the ABO blood group and Factor V Leiden in a Brazilian population

    Directory of Open Access Journals (Sweden)

    Magaly B.P.L.V. Lima

    2009-01-01

    Full Text Available Most cases of a predisposition to venous thrombosis are caused by resistance to activated protein C, associated in 95% of cases with the Factor V Leiden allele (FVL or R506Q. Several recent studies report a further increased risk of thrombosis by an association between the AB alleles of the ABO blood group and Factor V Leiden. The present study investigated this association with deep vein thrombosis (DVT in individuals treated at the Hemocentro de Pernambuco in northeastern Brazil. A case-control comparison showed a significant risk of thrombosis in the presence of Factor V Leiden (OR = 10.1, which was approximately doubled when the AB alleles of the ABO blood group were present as well (OR = 22.3. These results confirm that the increased risk of deep vein thrombosis in the combined presence of AB alleles and Factor V Leiden is also applicable to the Brazilian population suggesting that ABO blood group typing should be routinely added to FVL in studies involving thrombosis.

  19. Increased risk of venous thrombosis by AB alleles of the ABO blood group and Factor V Leiden in a Brazilian population

    Science.gov (United States)

    2009-01-01

    Most cases of a predisposition to venous thrombosis are caused by resistance to activated protein C, associated in 95% of cases with the Factor V Leiden allele (FVL or R506Q). Several recent studies report a further increased risk of thrombosis by an association between the AB alleles of the ABO blood group and Factor V Leiden. The present study investigated this association with deep vein thrombosis (DVT) in individuals treated at the Hemocentro de Pernambuco in northeastern Brazil. A case-control comparison showed a significant risk of thrombosis in the presence of Factor V Leiden (OR = 10.1), which was approximately doubled when the AB alleles of the ABO blood group were present as well (OR = 22.3). These results confirm that the increased risk of deep vein thrombosis in the combined presence of AB alleles and Factor V Leiden is also applicable to the Brazilian population suggesting that ABO blood group typing should be routinely added to FVL in studies involving thrombosis. PMID:21637678

  20. Seroprevalence of Helicobacter pylori in dyspeptic patients and its relationship with HIV infection, ABO blood groups and life style in a university hospital, Northwest Ethiopia

    Institute of Scientific and Technical Information of China (English)

    Feleke Moges; Afework Kassu; Getahun Mengistu; Solomon Adugna; Berhanu Andualem; Takeshi Nishikawa; Fusao Ota

    2006-01-01

    AIM: To determine the prevalence of Helicobacter pylori (H pylori) among dyspeptic patients and to assess the relationship between H pylori infection, blood group, HIV infection and life style of the patients.METHODS: In a hospital-based cross-sectional study,patients attending Outpatient Department of University of Gondar Hospital were enrolled. Socio-demographic information was collected using questionnaires. Serum was analyzed for anti-H pylori IgG antibodies using a commercial kit. HIV serostatus was determined by enzyme-linked immunosorbent assay (ELISA). Blood grouping was performed by slide agglutination tests.RESULTS: A total of 215 dyspeptic patients were included in the study. One hundred and sixteen patients (54%) were females and 99 (46%) were males. Anti-H pylori IgG antibodies were detected in sera of 184 (85.6%) patients. The prevalence was significantly higher in patients aged 50 years and above. Twenty point five percent of the patients were found to be seropositive for HIV. No significant association was found between sex,ABO blood groups, consumption of spicy diets, socioeconomic status and seropositivity for H pylori. However,alcohol consumption was significantly associated with H pylori serology.CONCLUSION: The prevalence of H pylori infection is associated with a history of alcohol intake and older age.The effect of different diet, alcohol and socioeconomic status as risk factors for H pylori infection needs further study.

  1. Distinct effects of pollinator dependence and self-incompatibility on pollen limitation in South African biodiversity hotspots.

    Science.gov (United States)

    Rodger, James G; Ellis, Allan G

    2016-06-01

    Global synthesis indicates that limitation of plant fecundity by pollen receipt (pollen limitation) is positively related to regional plant diversity and is higher for self-incompatible than self-compatible species. While self-incompatible species are always dependent on pollinating agents, self-compatible species may be pollinator-dependent or autofertile. This should cause variation in pollen limitation among self-compatible species, with lower pollen limitation in autofertile species because they do not depend on pollinators. We hypothesized that the intensity of pollen limitation in self-incompatible compared with pollinator-dependent self-compatible species should depend on whether pollen limitation is determined more by quantity than quality of pollen received. We compared pollen limitation between these three groups using a dataset of 70 biotically pollinated species from biodiverse regions of South Africa. Comparison with a global dataset indicated that pollen limitation in the South African biodiversity hotspots was generally comparable to other regions, despite expectations of higher pollen limitation based on the global plant diversity-pollen limitation relationship. Pollen limitation was lowest for autofertile species, as expected. It was also higher for pollinator-dependent self-compatible species than self-incompatible species, consistent with increased pollen-quality limitation in the former group due to negative consequences of pollinator-mediated self-pollination. However, there was a higher frequency of plants with zygomorphic flowers, which were also more pollen-limited, among pollinator-dependent self-compatible species. Thus, we could not attribute this difference in pollen limitation exclusively to a difference in pollen quality. Nevertheless, our results indicate that comparative studies should control for both pollinator dependence and self-incompatiblity when evaluating effects of other factors on pollen limitation. PMID:27277954

  2. COMPARISON OF PROPOFOL EFFECT ON PATIENTS WITH DIFFERENT BLOOD GROUPS%异丙酚在不同ABO血型病人的麻醉效果比较

    Institute of Scientific and Technical Information of China (English)

    于建设; 石海霞

    2012-01-01

    Objective: To compare the effect of propofol on patients with different blood groups. Methods:80 ASA Ⅰ or Ⅱ patients,age 40 -60year,weighing 50 ~75kg,scheduled for selected general anesthesia,were enrolled in this study. According to ABO blood -group system,the patients were divided into 4 groups(n =20) :group A,group B,group AB,group 0. After the patients in operation room coordinated 10 minutes, record the values of MAP, HR, BIS before administrating propofol(To) , then Propofol was administrated by TCI at an effect - site concentration of 4. 0μg/mLinitially, the target effect -site concentration was 6μg/mL. Record the values of MAP,HR,BIS at different time points of propofol effect - site concentration respectively, such as 4μg/mL ( T, ), 4. 5μg/mL ( T2), 5μg/mL (T3) ,5. 5μg/mL(T4). Measured the value distance( A ) of MAP, HR, BIS at each time point,compared with Toas baseline. Results: △MAP、△ HR were the highest in group B(P <0.01) ,the second in group( P <0.05 ) ,and group A and group B have no significance. Between T3 and T4, ABIS in group A was higher than the other blood groups. Conclusion: The effect of propofol is different on patients with different blood groups.%目的:探讨不同ABO血型病人是否对异丙酚的效果存在差异,以期对临床麻醉应用异丙酚提供个体化依据.方法:择期全麻手术病人80例,年龄40~60岁,体重50~75 kg,ASA分级Ⅰ或Ⅱ级,根据血型(n=20)分为A组、B组、AB组、O组.病人入室稳定10min后测定靶控输注异丙酚前T0的MAP、HR和BIS值,之后开始静脉血浆靶控输注异丙酚,初始浓度4μg/mL,目标浓度6μg/mL,分别在靶控浓度4μg/mL(T1)、4.5μg/mL(T2)、5 μg/mL( T3)、5.5μg/mL(T4)时测定MAP、HR、BIS值.分别计算每组用异丙酚后各时间点MAP、HR、BIS与麻醉前测定值的差值△.结果:各时间点△MAP、△HR比较,B组最高,(P<0.01),0组次之(P<0.05),而A组和AB组无明显差异.△BIS,只有A组在T3和T4时间

  3. Current topics in red cell biology: report on the Red Cell Special Interest Group meeting held at NHS Blood and Transplant Bristol on 30 October 2015.

    Science.gov (United States)

    Bullock, T; Bruce, L J; Ridgwell, K

    2016-08-01

    The Red Cell Special Interest Group (SIG) meeting, hosted by the British Blood Transfusion Society, provides an annual forum for the presentation of UK- and European-based red cell research. The 2015 meeting was held on Friday 30 October at the National Health Service Blood & Transplant (NHSBT) facility in Filton, Bristol and provided an exciting and varied programme on the themes of erythropoiesis, malaria biology and pathophysiology and red cells properties in stress and disease. Ten speakers presented on these topics over the course of one day. The meeting was well attended by over 90 delegates. Posters were presented during the lunch break, and abstracts from the posters are published at the end of this issue.

  4. Drug incompatibilities in the adult intensive care unit of a university hospital

    OpenAIRE

    Marsilio, Naiane Roveda; da Silva, Daiandy; Bueno, Denise

    2016-01-01

    Objectives This study sought to identify the physical and chemical incompatibilities among the drugs administered intravenously to patients admitted to an adult intensive care unit. We also aimed to establish pharmaceutical guidelines for administering incompatible drugs. Methods This cross-sectional, prospective, and quantitative study was conducted from July to September 2015. Drug incompatibilities were identified based on an analysis of the patient prescriptions available in the hospital ...

  5. Graft Incompatibility Influence on Assimilating Pigments and Soluble Sugars Amount of some Pear (Pyrus sativa) Cultivars

    OpenAIRE

    Gheorghii CIOBOTARI; Maria BRINZA; Aliona MORARIU; Gica GRADINARIU

    2010-01-01

    Graft incompatibility in fruit trees is one of the greatest obstacles in rootstocks and cultivars breeding. The mechanism in which incompatibility is expressed is not yet fully understood and several hypotheses have been advanced in an attempt to explain it. In many cases (pear on quince grafts, apricot on Prunus grafts), incompatibility is manifested by the breaking of the trees at the point of the union particularly when they have been growing for some years. Many reports focus on this prob...

  6. BLOOD DONATION

    CERN Document Server

    SC Unit

    2008-01-01

    A blood donation, organized by EFS (Etablissement Français du Sang) of Annemasse will take place On Wednesday 12 November 2008, from 8:30 to 16:00, at CERN Restaurant 2 If possible, please, bring your blood group Card.

  7. Grupos sanguíneos e lúpus eritematoso crônico discoide Blood groups and discoid lupus erythematosus

    Directory of Open Access Journals (Sweden)

    Andréia de Almeida Tamega

    2009-10-01

    Full Text Available FUNDAMENTOS: Lesão discoide é a manifestação cutânea mais comum do lúpus eritematoso, e formas cutâneas crônicas apresentam características imunológicas próprias, direcionadas ao polo Th1. Diversas doenças possuem associação com grupos sanguíneos, o que não foi ainda estudado no lúpus discoide. OBJETIVO: Investigar a associação entre tipos sanguíneos (ABO e Rh e lúpus eritematoso discoide. MÉTODOS: Estudo prospectivo tipo transversal envolvendo tipagem sanguínea ABO e Rh, inquérito de dados clínicos e dosagem de FAN e C4 de portadores de lúpus discoide sem critérios de doença sistêmica, atendidos em hospital universitário. RESULTADOS: Foram incluídos no estudo 69 pacientes, sendo 71,0% do sexo feminino (p 1:160, em 31,9%; e níveis baixos de C4, em 8,7%. Não houve diferença significativa entre as frequências dos grupos sanguíneos dos pacientes e da população local; entretanto, o grupo A foi associado às formas disseminadas da doença (OR 4,1 e p Background: Discoid lesion is the commonest cutaneous finding in lupus erythematosus and chronic types have their own immunological features, with Th1 inflammation profile. Although many diseases have association with blood-group systems, this fact was not enlightened in discoid lupus erythematosus. Objective: To investigate the association between blood groups (ABO and Rh and discoid lupus erythematosus. Methods: A prospective cross-sectional study assessing clinical information, blood group systems (ABO and Rh, FAN and C4 serum levels from discoid lupus patients without characteristics of systemic disease, was carried out at a clinic from a Brazilian university hospital. Results: Sixty-nine patients were enrolled in the study, 71.0% were females (p1:160 in 31.9%, and low levels of C4 in 8.7%. There was no significant difference between the frequency of blood groups from discoid lupus patients and local population, however, blood group A was associated to

  8. ABO and Rhesus Blood Groups and Risk of Endometriosis in a French Caucasian Population of 633 Patients Living in the Same Geographic Area

    Directory of Open Access Journals (Sweden)

    Bruno Borghese

    2014-01-01

    Full Text Available Objectives. The identification of epidemiological factors increasing the risk of endometriosis could shorten the time to diagnosis. Specific blood groups may be more common in patients with endometriosis. Study Design. We designed a cross-sectional study of 633 Caucasian women living in the same geographic area. Study group included 311 patients with histologically proven endometriosis. Control group included 322 patients without endometriosis as checked during surgery. Frequencies of ABO and Rhesus groups in the study and control groups were compared using univariate and multivariate analyses. Results. We observed a higher proportion of Rh-negative women in the study group, as compared to healthy controls. Multivariate analysis showed that Rh-negative women are twice as likely to develop endometriosis (aOR = 1.90; 95% CI: 1.20–2.90. There was no significant difference in ABO group distribution between patients and controls. There was no difference when taking into account either the clinical forms (superficial endometriosis, endometrioma, and deep infiltration endometriosis or the rAFS stages. Conclusion. Rh-negative women are twice as likely to develop endometriosis. Chromosome 1p, which contains the genes coding for the Rhesus, could also harbor endometriosis susceptibility genes.

  9. Psychological Reactions and Nursing Experience of Arterial Blood Groups%动脉采血人群的心理反应和护理体会

    Institute of Scientific and Technical Information of China (English)

    李丹丹; 滕彦; 汪琼; 陈力荣; 刘国生

    2014-01-01

    目的:探讨分析采集动脉血液时针对患者存在的不良心理的护理。方法:随机选取500例需要进行动脉血液采集的住院患者,给予心理问卷调查,分析其采集动脉血时的心理反应特点,探讨有针对性的心理护理干预措施。结果:各组患者均有不同的心理反应,不同组别人群经护理干预后行动脉血液采集的患者不良心理反应显著性改善,配合护理人员操作程度显著提高,穿刺成功率显著增加,与护理前比较,差异均具有统计学意义(P<0.05)。结论:根据患者不同的心理反应类型采取相应的心理护理,可使患者处于良好的心理状态,达到理想的动脉血液采集效果,提高动脉血液采集的护理质量。%Objective:To explore the nursing of adverse reaction in patients with arterial blood collection exists.Method:500 cases of need for arterial blood collection in hospitalized patients were randomly selected,they were provided psychological questionnaires to analyze the characteristics of their psychological reactions when collecting blood,and to explore the psychological care interventions targeted.Result:There were different adverse psychological reactions in each groups.Different groups of the population by the nursing intervention in patients with arterial blood collection underwent adverse psychological reactions were significantly improved,with significantly improved operating nursing degree,a significant increase in the success rate,compared with before treatment,the differences were statistically significant(P<0.05).Conclusion:According to the patient ’s psychological reaction types corresponding taking different psychological care,patients can be in a good state of mind,to achieve the desired effect of arterial blood collection and improve the quality of care arterial blood collection.

  10. ABO-incompatible living-donor pediatric kidney transplantation in Japan

    Directory of Open Access Journals (Sweden)

    Atsushi Aikawa

    2014-01-01

    Full Text Available The Japanese ABO-Incompatible Transplantation Committee officially collected and analyzed data on pediatric ABO-incompatible living-donor kidney transplantation in July 2012. The age of a child was defined as <16 years, and 89 children who had undergone ABO-incompatible living-donor kidney transplantation from 1989 to 2011 were entered in a registry. These data were presented as the Japanese registry of pediatric ABO-incompatible living-donor kidney transplantation at the regional meetings of the International Pediatric Transplantation Association (IPTA in Nagoya in September 2012 and in Sao Paulo in November 2012.

  11. Application of fully automated blood grouping analyzer in the blood donor testing%全自动血型分析仪应用于献血者血型筛查

    Institute of Scientific and Technical Information of China (English)

    周国平; 周结; 向东; 谢云峥; 杨军; 郑岚; 曹斌; 吴蓉晖

    2011-01-01

    Objective To evaluate the performance of a fully automated blood grouping analyzer for ABO and RhD screening and the red blood cell IgM unexpected antibody detection. Methods A total of 25 554 samples were collected from blood donors. ABO, RhD, and IgM unexpected antibodies were tested simultaneously by fully automated blood grouping analyzer and manual colorimetric method with semi-automated sampler. For discrepancies between forward and reverse ABO grouping, agglutination of O cells, RhD negative results, samples would be referred to the reference laboratory of Shanghai Blood Center for further identification. Results The accuracy rates of ABO grouping by fully automated blood grouping analyzer and manual colorimetric method with semi-automated sampler were 99.93% (25 535/25 554) and 99.95% (25 542/25 554)respectively(P > 0.05 ) ;the rates of agglutination of O cells were 0.18% (46/25 554), and 0.10% (26/25 554) (P <0.05) respectively;the ABO forward and reverse grouping discrepancies were 17(0.06% ) and 10(0.04% ) ,respectively. Reference lab confirmed that there were 5 subgroups discovered by both methods ;2 subgroups were missed by each method(0.01% ) ,the rest were normal ABO blood group specimens (10/17 vs 3/10, P > 0.05). Conclusion The fully automated blood grouping analyzer can perform blood donor testing with high accuracy, high standardization in operation, and easier identification of IgM irregular antibodies.%目的 探讨并评价全自动血型分析仪应用于献血者血型筛查和盐水不规则抗体检测.方法 采用全自动血型分析仪(全自动法)对25 554例献血者标本作ABO及BhD血型鉴定、盐水不规则抗体初筛,并与加样仪加样手工比色法(半自动法)作比对实验.ABO正反定型不一致而无法定型、O细胞凝集、RhD阴性的标本送血型红细胞参比实验室鉴定.结果 全自动法与半自动法比较,ABO、RhD阴性血型1次准确定型率:99.93%(25 535/25 554)vs 99

  12. Significance of irradiation of blood

    International Nuclear Information System (INIS)

    Many reports of fatal GVHD occurring in non-immunocompromised patients after blood transfusion have been published in Japan. One explantation is that transfused lymphocytes were simulated and attack the recipient organs recognized as HLA incompatible. That is so called 'one-way matching'. To reduce the risk of post-transfusion GVHD, one of the most convenient methods is to irradiate the donated blood at an appropriate dose for inactivation of lymphocytes. Because no one knows about the late effect of irradiated blood, it is necessary to make the prospective safety control. (author)

  13. Detection rate and distribution of unexpected antibody of RBC blood group among blood donors%献血人群红细胞血型意外抗体的检出率及分布情况

    Institute of Scientific and Technical Information of China (English)

    宋任浩; 何路军; 常缨

    2014-01-01

    Objective To understand the occurrence frequency and distribution charactristics of unexpected an-tibody of RBC blood group among blood donors in this area to provide the theoretical basis for improving blood trans -fusion safety .Methods Unexpected antibody screening of RBC blood group was conducted in healthy blood donors in our center from January to December 2012 .The positive donors with antibody screening were performed the further specificity identification and their occurrence frequency and distribution characteristics were analyzed .Results A-mong 195 404 blood samples ,59 cases of unexpected antibodies were screened out with the positive rate of 0 .030% . 52 cases were IgM antibody with the positive rate of 0 .027% ;7 cases were IgG antibody with the positive rate of 0 .004% .The positive rate of women was higher than that of men ,the difference had statistical significance .36 cases of specific antibody were detected ,accounting for 61 .02% ,23 cases were nonspecific antibody ,accounting for 38 .98% .Conclusion Certain detection rate of unexpected antibodies exists among blood donors .Unexpected anti-body screening should be included in the routine test items for ensuring the safety of blood transfusion .%目的:了解该地区献血人群红细胞血型意外抗体的发生频率和分布特点,为提高用血安全提供理论依据。方法对2012年1~12月健康献血者进行意外抗体筛查,抗体筛查阳性者做进一步特异性鉴定,并分析其发生频率和分布特点。结果在195404例血液标本中,共检出意外抗体59例,阳性率为0.030%。其中 IgM 抗体52例,阳性率为0.027%;IgG 抗体7例,阳性率为0.004%,男女性别间阳性率比较,女性高于男性,差异均有统计学意义(P<0.05)。共检出特异性抗体36例,占61.02%,非特异性抗体23例,占38.98%。结论献血人群有一定意外抗体检出率,意外抗体筛查应列入献血者常

  14. Blood Type Puzzle.

    Science.gov (United States)

    Kelly, Janet

    1997-01-01

    Presents a blood type puzzle that provides a visual, hands-on mechanism by which students can examine blood group reactions. Offers students an opportunity to construct their own knowledge about blood types. (JRH)

  15. High blood pressure - infants

    Science.gov (United States)

    National High Blood Pressure Education Program Working Group on High Blood Pressure in Children and Adolescents. The fourth report on the diagnosis, evaluation, and treatment of high blood pressure in children and adolescents. Pediatrics . ...

  16. The O-Linked Glycome and Blood Group Antigens ABO on Mucin-Type Glycoproteins in Mucinous and Serous Epithelial Ovarian Tumors.

    Directory of Open Access Journals (Sweden)

    Varvara Vitiazeva

    Full Text Available Mucins are heavily O-glycosylated proteins where the glycosylation has been shown to play an important role in cancer. Normal epithelial ovarian cells do not express secreted mucins, but their abnormal expression has previously been described in epithelial ovarian cancer and may relate to tumor formation and progression. The cyst fluids were shown to be a rich source for acidic glycoproteins. The study of these proteins can potentially lead to the identification of more effective biomarkers for ovarian cancer.In this study, we analyzed the expression of the MUC5AC and the O-glycosylation of acidic glycoproteins secreted into ovarian cyst fluids. The samples were obtained from patients with serous and mucinous ovarian tumors of different stages (benign, borderline, malignant and grades. The O-linked oligosaccharides were released and analyzed by negative-ion graphitized carbon Liquid Chromatography (LC coupled to Electrospray Ionization tandem Mass Spectrometry (ESI-MSn. The LC-ESI-MSn of the oligosaccharides from ovarian cyst fluids displayed differences in expression of fucose containing structures such as blood group ABO antigens and Lewis-type epitopes.The obtained data showed that serous and mucinous benign adenomas, mucinous low malignant potential carcinomas (LMPs, borderline and mucinous low-grade carcinomas have a high level of blood groups and Lewis type epitopes. In contrast, this type of fucosylated structures were low abundant in the high-grade mucinous carcinomas or in serous carcinomas. In addition, the ovarian tumors that showed a high level of expression of blood group antigens also revealed a strong reactivity towards the MUC5AC antibody. To visualize the differences between serous and mucinous ovarian tumors based on the O-glycosylation, a hierarchical cluster analysis was performed using mass spectrometry average compositions (MSAC.Mucinous benign and LMPs along with mucinous low-grade carcinomas appear to be different from

  17. An Atypical Clostridium Strain Related to the Clostridium botulinum Group III Strain Isolated from a Human Blood Culture

    OpenAIRE

    Bouvet, Philippe; Ruimy, Raymond; Bouchier, Christiane; Faucher, Nathalie; Mazuet, Christelle; Popoff, Michel R.

    2014-01-01

    A nontoxigenic strain isolated from a fatal human case of bacterial sepsis was identified as a Clostridium strain from Clostridium botulinum group III, based on the phenotypic characters and 16S rRNA gene sequence, and was found to be related to the mosaic C. botulinum D/C strain according to a multilocus sequence analysis of 5 housekeeping genes.

  18. Blood Component Therapy and Coagulopathy in Trauma: A Systematic Review of the Literature from the Trauma Update Group

    Science.gov (United States)

    Poole, Daniele; Cortegiani, Andrea; Chieregato, Arturo; Russo, Emanuele; Pellegrini, Concetta; De Blasio, Elvio; Mengoli, Francesca; Volpi, Annalisa; Grossi, Silvia; Gianesello, Lara; Orzalesi, Vanni; Fossi, Francesca; Chiara, Osvaldo; Coniglio, Carlo; Gordini, Giovanni

    2016-01-01

    Background Traumatic coagulopathy is thought to increase mortality and its treatment to reduce preventable deaths. However, there is still uncertainty in this field, and available literature results may have been overestimated. Methods We searched the MEDLINE database using the PubMed platform. We formulated four queries investigating the prognostic weight of traumatic coagulopathy defined according to conventional laboratory testing, and the effectiveness in reducing mortality of three different treatments aimed at contrasting coagulopathy (high fresh frozen plasma/packed red blood cells ratios, fibrinogen, and tranexamic acid administration). Randomized controlled trials were selected along with observational studies that used a multivariable approach to adjust for confounding. Strict criteria were adopted for quality assessment based on a two-step approach. First, we rated quality of evidence according to the Grading of Recommendations Assessment, Development and Evaluation (GRADE) criteria. Then, this rating was downgraded if other three criteria were not met: high reporting quality according to shared standards, absence of internal methodological and statistical issues not detailed by the GRADE system, and absence of external validity issues. Results With few exceptions, the GRADE rating, reporting and methodological quality of observational studies was “very low”, with frequent external validity issues. The only two randomized trials retrieved were, instead, of high quality. Only weak evidence was found for a relation between coagulopathy and mortality. Very weak evidence was found supporting the use of fibrinogen administration to reduce mortality in trauma. On the other hand, we found high evidence that the use of 1:1 vs. 1:2 high fresh frozen plasma/packed red blood cells ratios failed to obtain a 12% mortality reduction. This does not exclude lower mortality rates, which have not been investigated. The use of tranexamic acid in trauma was supported by

  19. Multiple components of blood group A and B antigens in human erythrocyte membranes and their difference between A1 and A2 status.

    OpenAIRE

    Fujii, H.; Yoshida, A.

    1980-01-01

    Human type O erythrocyte membranes were converted to type A1 by purified human A1-enzyme, to type A2 by purified human A2-enzyme, and to type B by purified human B-enzyme in the presence of radioactive sugar donors (i.e., UDP-N-acetylgalactosamine for A-enzyme and UDP-galactose for B-enzyme, respectively). Type A2 erythrocyte membranes were also converted to type A1 by purified A1-enzyme A1-enzyme. The labeled blood group antigens (A1, A2, and B) thus produced were analyzed by sodium dodecyl ...

  20. Cloning of glycoprotein D cDNA, which encodes the major subunit of the Duffy blood group system and the receptor for the Plasmodium vivax malaria parasite.

    OpenAIRE

    Chaudhuri, A; Polyakova, J; Zbrzezna, V; K Williams; Gulati, S.; Pogo, A O

    1993-01-01

    cDNA clones encoding the major subunit of the Duffy blood group were isolated from a human bone marrow cDNA library using a PCR-amplified DNA fragment encoding an internal peptide sequence of glycoprotein D (gpD) protein. The open reading frame of the 1267-bp cDNA clone indicated that gpD protein was composed of 338 amino acids, predicting a M(r) of 35,733, which was the same as a deglycosylated gpD protein. Portions of the predicted amino acid sequence, matched with six CNBr/pepsin peptides ...

  1. Biochemical Models for S-Rnase-Based Self-Incompatibility

    Institute of Scientific and Technical Information of China (English)

    Zhi-Hua Hua; Allison Fields; Teh-hui Kao

    2008-01-01

    S-RNase-based self-incompatibility (SI) is a genetically determined self/non-self-recognition process employed by many flowering plant species to prevent inbreeding and promote outcrosses.For the Plantaginaceae,Rosa-ceae and Solanaceae,it is now known that S-RNase and S-Iocu F-box(two multiple allelic genes at the S-locus)determine the female and male specificity,respectively,during SI interactions.However,how allelic products of these two genes interact inside pollen tubes to result in specific growth inhibition of self-pollen tubes remains to be investigated.Here,we review all the previously proposed biochemical models and discuss whether their predictions are consistent with all SI phenomena,including competitive jnteraction where SI breaks down in pollen that carries two different pollen 5-alleles.We also discuss these models in Iight of the recent findings of compartmentalization of S-RNases in both incompatible and compatible pollen tubes.Lastly,we summarize the results from our recent biochemical studies of PiSLF(Petunia inflata SLF)and S-RNase.and present a new model for the biochemical mechanism of SI in the Solanaceae.The tenet of this model is that a PiSLF preferentially interacts with its non-self S-RNases in the cytoplasm of a pollen tube to result in the assembly of an E3-like complex,which then mediates ubiquitination and degradation of non-self S-RNases through the ubiquitin-26S proteasome pathway.This model can explain all SI phenomena and,at the same time,has raised new questions for further study.

  2. Incompatibility behavior of a symbiotic plasmid pMH7653Rb in Mesorhizobium huakuii 7653R

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Mesorhizobium huakuii strain 7653R harbored two indigenous plasmids named pMH7653Ra and pMH7653Rb.The larger plasmid pMH7653Rb (symbiotic plasmid) was transferred to M.huakuii HN308SR harboring three plasmids: pMHHN308a,pMHHN308b and pMHHN308c,and HN3015SR harboring three plasmids: pMHHN3015a,pMHHN3015b and pMHHN3015c by tri-parent mating.Two stable indigenous plasmids,pMHHN308b and pMHHN308c of HN308SR,were co-eliminated due to the introduction of pMH7653Rb,and the transconjugant was named HN308SRN14.The results implied that pMH7653Rb and pMHHN308b,pMHHN308c were incompatible and might have been ascribed to the same incompatible group.The plasmid profiles of transconjugant HN3015SRN14 showed that the second largest plasmid pMHHN3015b of HN3015SR was cured due to the introduction of pMH7653Rb.The results also implied that pMH7653Rb and pMHHN3015b were incompatible.Results from plant nodulation tests showed that pMH7653Rb could only maintain the nodulation ability in transconjugant HN308SRN14 and its nodule number was more than that of wild strain HN308SR,but could not replace the nitrogen fixation effect of pMHHN308b and pMHHN308c.The plasmid cured mutant HN308SRN14D harboring only pMHHN308a formed null nodules that demonstrated pMHHN308a was relevant to nodulation ability.HN3015SRN14 harboring pMH7653Rb,pMHHN3015a and pMHHN3015c formed null nodules while HN3015SRN14D containing pMHHN3015a and pMHHN3015c lost the nodulation ability.The plasmid replication repC-like gene sequences were detected by a polymerase chain reaction from 7653R,HN308,HN3015,HN308SRN14 and HN3015SRN14.The repC gene sequence similarities of the strains tested attained 99%.

  3. Detection of Lewis, P1, and some MNS blood group system antibodies by a solid phase assay.

    Science.gov (United States)

    Rolih, S; Thomas, R; Sinor, L

    1995-01-01

    Some solid phase red cell adherence (SPRCA) assays are designed to detect IgG antibodies to red blood cell (RBC) antigens. These assays use anti-IgG-coated red cells as the indicator. It is reported that most antibodies to Lea, Leb, P1, M, and N fail to react by solid phase (SP), presumably because they are IgM antibodies. Those detected are assumed to be IgG. In one year, during routine testing using SPRCA to screen patients for intended RBC transfusion, 28 of 59 such examples were found to react: anti-Lea(9), -Leb(1), -M(14), -N(1), and -P1(3). A study was undertaken to determine if reactivity was due to crosslinking by IgM antibodies of antigen-positive indicator RBCs to antigen-positive reagent RBC monolayers, or due to detection of IgG antibodies. Antibodies were tested according to standard SP protocols, except where IgG-neutralized indicator RBCs were substituted for anti-IgG-active indicator cells. The 59 samples were retested with antigen-positive and antigen-negative indicator RBCs. Only 5 of 59 reacted optimally when antigen-positive indicator cells were used: anti-Lea(2), -Leb(1), -M(1), and -N(1). The reactions of all antibodies were abolished when the anti-IgG component of the indicator was neutralized by soluble IgG. These findings show that detection of most Lewis, P1, M, and N antibodies by SPRCA is dependent on the presence of an IgG antibody in the serum.

  4. Pilot conversion trial from mycophenolic acid to everolimus in ABO-incompatible kidney-transplant recipients with BK viruria and/or viremia.

    Science.gov (United States)

    Belliere, Julie; Kamar, Nassim; Mengelle, Catherine; Allal, Asma; Sallusto, Federico; Doumerc, Nicolas; Game, Xavier; Congy-Jolivet, Nicolas; Esposito, Laure; Debiol, Benedicte; Rostaing, Lionel

    2016-03-01

    Immunosuppression using everolimus (EVR) plus low-dose tacrolimus (Tac) is commonly used in organ transplantation. EVR has potential antiviral effects. Herein, the long-term outcomes and impacts of Tac-EVR on the BK virus are reported in ABO-incompatible kidney-transplant recipients. The initial immunosuppressive regimen combined steroids, Tac, and mycophenolic acid (MPA). At a median of 141 (34-529) days post-transplantation, seven stable ABO-incompatible kidney-transplant recipients were converted from MPA to EVR because of active BK replication, and compared with a reference group of fourteen ABO-incompatible patients receiving classical Tac plus MPA. At 1 month before conversion, at 1, 3 months after, and at last follow-up, clinical and biological parameters were monitored. The median time from conversion to the last follow-up was 784 (398-866) days. Conversion to EVR caused no change to rejection episodes or immunological status (isoagglutinin titers, anti-HLA antibodies). At last follow-up, median eGFR was similar in the Tac-MPA versus Tac-EVR group (40 [range: 14-56] vs. 54.5 ml/min/1.73 m(2) [range: 0-128], P = 0.07). The major adverse event was dyslipidemia. Interestingly, conversion from MPA to EVR decreased BK viral load in five patients. ABO-incompatible kidney-transplant recipients with an active BK virus infection may benefit from conversion to EVR. PMID:26575959

  5. Can a Single Session of a Community-Based Group Exercise Program Combining Step Aerobics and Bodyweight Resistance Exercise Acutely Reduce Blood Pressure?

    Directory of Open Access Journals (Sweden)

    Mendes Romeu

    2014-12-01

    Full Text Available This study aimed to analyze the acute effects of a single session of a community-based group exercise program combining step aerobics and bodyweight resistance exercise on blood pressure in healthy young adult women. Twentythree healthy young adult women (aged 31.57 ± 7.87 years participated in two experimental sessions (exercise and control in a crossover study design. Blood pressure was monitored before, immediately after and at 10, 20 and 30 min of recovery. The exercise session consisted of four phases: 1 a warm-up (5 min of dance aerobics; 2 aerobic exercise training (30 min of step aerobics; 3 resistance exercise training (six sets of 12 repetitions of three bodyweight exercises in a circuit mode, 10 min; and 4 a cool-down (5 min of breathing and flexibility exercises; totaling 50 min of duration. Systolic blood pressure after exercise was significantly lower compared to control at the 10th min (-10.83 ± 2.13 vs. -2.6 ± 2.13 mmHg; p = 0.009, 20th min (-11.26 ± 2.13 vs. -3.04 ± 2.13 mmHg; p = 0.009 and 30th min of recovery (-10.87 ± 2.39 vs. -0.48 ± 2.39 mmHg; p = 0.004. A single session of a community-based group exercise program combining step aerobics and bodyweight resistance exercise was effective in inducing significant post-exercise hypotension in healthy young adult women. This type of low-cost exercise interventions may have an important role in the prevention of cardiovascular diseases and in community health promotion.

  6. The Hidden Conformation of Lewis x, a Human Histo-Blood Group Antigen, Is a Determinant for Recognition by Pathogen Lectins.

    Science.gov (United States)

    Topin, Jérémie; Lelimousin, Mickaël; Arnaud, Julie; Audfray, Aymeric; Pérez, Serge; Varrot, Annabelle; Imberty, Anne

    2016-07-15

    Histo-blood group epitopes are fucosylated branched oligosaccharides with well-defined conformations in solution that are recognized by receptors, such as lectins from pathogens. We report here the results of a series of experimental and computational endeavors revealing the unusual distortion of histo-blood group antigens by bacterial and fungal lectins. The Lewis x trisaccharide adopts a rigid closed conformation in solution, while crystallography and molecular dynamics reveal several higher energy open conformations when bound to the Ralstonia solanacearum lectin, which is in agreement with thermodynamic and kinetic measurements. Extensive molecular dynamics simulations confirm rare transient Le(x) openings in solution, frequently assisted by distortion of the central N-acetyl-glucosamine ring. Additional directed molecular dynamic trajectories revealed the role of a conserved tryptophan residue in guiding the fucose into the binding site. Our findings show that conformational adaptation of oligosaccharides is of paramount importance in cell recognition and should be considered when designing anti-infective glyco-compounds. PMID:27198630

  7. Blood transfusion in the para-Bombay phenotype.

    Science.gov (United States)

    Lin-Chu, M; Broadberry, R E

    1990-08-01

    The H-deficient phenotypes found in Chinese so far, have all been secretors of soluble blood group substances in saliva. The corresponding isoagglutinin activity (e.g. anti-B in OB(Hm) persons) has been found to be weak in all cases. To determine the clinical significance of these weak isoagglutinins 51Cr red cell survival tests were performed on three OB(Hm) individuals transfused with small volumes (4 ml) of groups B and O RBC. Rapid destruction of most of the RBC occurred whether or not the isoagglutinins of the OB(Hm) individuals were indirect antiglobulin test (IAGT) reactive. When a larger volume (54 ml packed RBC) of group B cells (weakly incompatible by IAGT) was transfused to another OB(Hm) individual with IAGT active anti-HI, the survival of the transfused RBC was 93% at 24 h, with 30% of the RBC remaining in the circulation at 28 d in contrast to 76% as would be expected if the survival was normal. Therefore when whole units of blood of normal ABO blood groups, compatible by IAGT, are transfused, the survival is expected to be almost normal. These weak isoagglutinins may not be very clinically significant and we suggest that when para-Bombay blood is not available, the compatibility testing for OA(Hm) persons should be performed with group A and group O packed RBC; OB(Hm) with group B and group O packed RBC: OAB(Hm) with groups A, B, AB and O packed RBC. For cross matching, the indirect antiglobulin test by a prewarmed technique should be used.

  8. 40 CFR 267.17 - What are the requirements for managing ignitable, reactive, or incompatible wastes?

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false What are the requirements for managing ignitable, reactive, or incompatible wastes? 267.17 Section 267.17 Protection of Environment ENVIRONMENTAL... requirements for managing ignitable, reactive, or incompatible wastes? (a) You must take precautions to...

  9. Within- and between-population variation for Wolbachia-induced reproductive incompatibility in a haplodiploid mite

    NARCIS (Netherlands)

    F. de Freitas Vala Salvador; A. Weeks; D. Claessen; J.A.J. Breeuwer; M.W. Sabelis

    2002-01-01

    Wolbachia pipientis is a bacterium that induces cytoplasmic incompatibility (CI), the phenomenon in which infected males are reproductively incompatible with uninfected females. CI spreads in a population of hosts because it reduces the fitness of uninfected females relative to infected females. CI

  10. Complex Self-Incompatibility Systems in Ranunculus acris L. and Beta vulgaris L

    DEFF Research Database (Denmark)

    Lundqvist, A.; Østerbye, U.; Larsen, K.;

    1973-01-01

    acris, and in Beta vulgaris there are at least four. The observations strongly support the theory of the incompatibility genes being ancient constituents of the breeding systems of the angiosperms. Most probably a complex type of incompatibility control was already present at the presumed common...

  11. Natural variation at Strubbelig Receptor Kinase 3 drives immune-triggered incompatibilities between Arabidopsis thaliana accessions

    NARCIS (Netherlands)

    Alcazar, R.; Garcia, A.V.; Kronholm, I.; Meaux, De J.; Koornneef, M.; Parker, J.E.; Reymond, M.

    2010-01-01

    Accumulation of genetic incompatibilities within species can lead to reproductive isolation and, potentially, speciation. In this study, we show that allelic variation at SRF3 (Strubbelig Receptor Family 3), encoding a receptor-like kinase, conditions the occurrence of incompatibility between Arabid

  12. Population genetics of self-incompatibility in a clade of relict cliff-dwelling plant species

    Science.gov (United States)

    Silva, Jose L.; Brennan, Adrian C.; Mejías, José A.

    2016-01-01

    The mating systems of species in small or fragmented populations impact upon their persistence. Small self-incompatible (SI) populations risk losing S allele diversity, responsible for the SI response, by drift thereby limiting mate availability and leading to population decline or SI system breakdown. But populations of relict and/or endemic species have resisted these demographic conditions over long periods suggesting their mating systems have adapted. To address a lack of empirical data on this topic, we studied the SI systems of three relict cliff-dwelling species of Sonchus section Pustulati (Asteraceae): S. masguindalii, S. fragilis and S. pustulatus in the western Mediterranean region. We performed controlled pollinations within and between individuals to measure index of SI (ISI) expression and identify S alleles in multiple population samples. Sonchus masguindalii and S. pustulatus showed strong SI (ISI = 0.6–1.0) compared to S. fragilis (ISI = 0.1–0.7). Just five S alleles were estimated for Spanish S. pustulatus and a moderate 11-15 S alleles for Moroccan S. pustulatus and S. fragilis, respectively. The fact that autonomous fruit set was generally improved by active self-pollination in self-compatible S. fragilis suggests that individuals with weak SI can show a wide range of outcrossing levels dependent on the degree of self or outcross pollen that pollinators bear. We conclude that frequent S allele dominance interactions that mask the incompatibility interactions of recessive S alleles leading to higher mate availability and partial breakdown of SI leading to mixed mating, both contribute to reproductive resilience in this group. PMID:27154621

  13. Population genetics of self-incompatibility in a clade of relict cliff-dwelling plant species.

    Science.gov (United States)

    Silva, Jose L; Brennan, Adrian C; Mejías, José A

    2016-01-01

    The mating systems of species in small or fragmented populations impact upon their persistence. Small self-incompatible (SI) populations risk losing S allele diversity, responsible for the SI response, by drift thereby limiting mate availability and leading to population decline or SI system breakdown. But populations of relict and/or endemic species have resisted these demographic conditions over long periods suggesting their mating systems have adapted. To address a lack of empirical data on this topic, we studied the SI systems of three relict cliff-dwelling species of Sonchus section Pustulati (Asteraceae): S. masguindalii, S. fragilis and S. pustulatus in the western Mediterranean region. We performed controlled pollinations within and between individuals to measure index of SI (ISI) expression and identify S alleles in multiple population samples. Sonchus masguindalii and S. pustulatus showed strong SI (ISI = 0.6-1.0) compared to S. fragilis (ISI = 0.1-0.7). Just five S alleles were estimated for Spanish S. pustulatus and a moderate 11-15 S alleles for Moroccan S. pustulatus and S. fragilis, respectively. The fact that autonomous fruit set was generally improved by active self-pollination in self-compatible S. fragilis suggests that individuals with weak SI can show a wide range of outcrossing levels dependent on the degree of self or outcross pollen that pollinators bear. We conclude that frequent S allele dominance interactions that mask the incompatibility interactions of recessive S alleles leading to higher mate availability and partial breakdown of SI leading to mixed mating, both contribute to reproductive resilience in this group.

  14. Identification and Clinical Infusion of B (A) Blood Group%B(A)血型的鉴定及临床输注探讨

    Institute of Scientific and Technical Information of China (English)

    郑望春; 骆宏; 叶有玩; 沈磁石; 黄华霖

    2013-01-01

    目的 对1例常规血清学为B(A)表现型的献血者进行血型鉴定,探讨B(A)血型在临床输注中的安全性和有效性.方法 采用血型血清学、ABO基因第6及第7外显子直接测序和克隆测序的方法,对该例标本的血清学表型和其ABO等位基因进行检测;同时回顾性调查该献血者前次已献血液输注给B型受血者的相关实验记录和病历.结果 被检血样的血型血清学结果初步定型为B(A);直接测序和克隆测序发现:被检者含有O01等位基因,B等位基因在第7外显子存在640A/G突变,证实其为B(A)04等位基因,其基因型为B(A)04/O01;该献血者血液输给B型受血者,Hb升高符合预期值,无输血不良反应发生.结论 发现该例B(A)表现型,其基因型为B(A)04/O01型;在配血相合的情况下,B(A)供血者血液输注给B型受血者是安全有效的.%Objective To explore the identification and clinical infusion of B(A) blood group. Methods B(A) blood groups and B(A) alleles of the samples were checked out by serology and direct sequencing and cloning sequencing of ABO gene exon 6 and 7. Retrospective experiment records were collected to analyze the clinical infusion. Results The serological results of the blood sample was shown as B(A). Result of the sequencing analysis showed the sample harbored an O01 allele.and had the nt640 A/G mutation in B gene,which was previously defined as B(A) 04. B(A) blood of donor was infused to type B receptor,whose Hb rising conformed to the expected value, no adverse reaction of blood transfusion occurred. Conclusion The sample is identified as B(A) phenotype in serological tests, whose genotype is rare B(A)04 / O01. Under the consistency of cross matching.it is safe and effective for the blood of B (A) blood donor infused to type B receptor.

  15. Prevalence of antinuclear antibodies in 3 groups of healthy individuals: blood donors, hospital personnel, and relatives of patients with autoimmune diseases.

    Science.gov (United States)

    Marin, Guadalupe G; Cardiel, Mario H; Cornejo, Horacio; Viveros, Martha E

    2009-10-01

    Antinuclear antibodies (ANA) are frequently found in healthy populations. To define the prevalence, pattern, and titer of ANA in different groups of the healthy Mexican population, we studied 304 individuals, classified into 3 groups: 104 blood donors, 100 hospital personnel working at The State General Hospital, which included doctors, laboratory technicians, and nurses; and 100 relatives of patient diagnosed either with systemic lupus erythematosus or rheumatoid arthritis, all of them apparently healthy at the time of study. We determined ANA using immunofluorescence microscopy performed on HEp-2 cells. Fluorescence was detected in 165 serum samples (54.3%). The most frequent pattern was the speckled (50.3%). The most frequent dilution was 1:40 (35.4%), followed by 1:80 (13.4%), 1:160 (3.2%), and 1:320 (1.3%).Regarding the results by study group, we found a trend toward higher ANA levels in group 2 (hospital personnel), compared with group 1 (blood donors) and group 3 (relatives of patients), a trend also reflected by the increasing frequency of serum titers of 1:80 and higher (P = 0.074). According to occupation, medical doctors showed a higher incidence of speckled pattern when compared with other occupations (P = 0.022). Medical doctors (n = 75) showed also higher titers of this particular pattern (P = 0.03). In group 3, relatives of patients with systemic lupus erythematosus showed the speckled pattern more frequently than relatives of patients with rheumatoid arthritis, in low titers (P = 0.017). We suggest that ANA tests showing speckled pattern should be at a 1:160 titer or higher to be considered positive; other patterns such as homogeneous, peripheral, or centromeric might be considered positive even at low titers (

  16. Auto-incompatibilidade em plantas Self-incompatibility in plants

    Directory of Open Access Journals (Sweden)

    Maria Teresa Schifino-Wittmann

    2002-12-01

    Full Text Available A auto-incompatibilidade (AI é a incapacidade de uma planta fértil formar sementes quando fertilizada por seu próprio pólen. É um mecanismo fisiólogico, com base genética, que promove a alogamia, e tem despertado a atenção de geneticistas e melhoristas de plantas. Atualmente, a ênfase nas pesquisas está na identificação e entendimento dos processos moleculares e celulares que levam ao reconhecimento e à rejeição do pólen auto-incompatível, incluindo a identificação, localização e seqüenciamento das proteínas, enzimas e genes envolvidos. Existem dois tipos principais de AI, a gametofítica (AIG, em que a especificidade do pólén é gerada pelo alelo S do genoma haplóide do grão do pólen (gametófito, e a esporofítica (AIE, em que a especificidade é gerada pelo genótipo diplóide da planta adulta (esporófito que deu origem ao grão de pólen. A AIE pode ser homomórfica, quando não existem modificações florais que acompanham o processo, ou heteromórfica, quando, com o processo de AI, ocorrem modificações florais. A reação da AI engloba desde o impedimento da germinação do pólen até o rompimento do tubo polínico. A ocorrência de AI em espécies de interesse econômico pode ter uma importância muito grande, sendo muito positiva em alguns casos e um empecilho em outros, dependendo da parte da planta (vegetativa ou reprodutiva que é colhida e do tipo de reprodução, sexual ou vegetativa. A utilização da AI no melhoramento de plantas é feita há bastante tempo, mas existe uma lacuna entre o grau de detalhamento do conhecimento teórico, como as bases genética e molecular, e a aplicação deste conhecimento no melhoramento.Self-incompatibility (SI is the failure of a fertile plant to set seeds when fertilized with its own pollen. It is a physiological mechanism, with a genetic basis, which promotes allogamy and has drawn geneticists and plant breeders’ attention. Nowadays, the research has put

  17. Discrepancy of B cell frequency between periphery and spleen after rituximab treatment in ABO-incompatible liver transplantation.

    Science.gov (United States)

    Iso, Yukihiro; Sawada, Tokihiko; Kita, Junji; Shiraki, Takayuki; Sakuraoka, Yuki; Kato, Masato; Shimoda, Mitsugi; Kubota, Keiichi

    2013-10-01

    ABO-incompatible living-donor liver transplantation (ABO-LDLT) is generally more difficult to perform than ABO-incompatible kidney transplantation. Despite introduction of rituximab, ABO-LDLT in non-responders is a still difficult issue. A 23-year-old woman with primary sclerosing cholangitis underwent LDLT. The recipient's blood type was 0(+) and the donor's was B(+). Rituximab was infused twice on preoperative day (POD) 14 and 7. Plasma exchange (PE) was performed on PODs 5, 3, 2, and 1. However, repeated PE failed to decrease the anti-B antibody titer. On the other hand, preoperative esophagogastroscopy revealed esophageal varices with red color sign. Therefore, simultaneous liver transplantation and Hassab operation were performed. The donor left lobe of the liver was orthotopically transplanted into the recipient following Hassab operation. Flow cytometry on the day of surgery showed that the frequencies of B cells (CD20+) and memory B cells (CD20+/CD27+) in the peripheral blood were 0.9% and 0.3%, respectively; flow cytometry of cells recovered from the spleen revealed that the frequencies of B cells and memory B cells were 2.5% and 2.4%, respectively. Acute cellular rejection occurred on POD 15, and was treated by steroid pulse therapy, leading to a decrease in the anti-B antibody titer. The liver was functioning well on POD 390 (AST 19, ALT 34). In non-responders to ABO-LDLT, anti-donor blood type antibody-producing cells remains in the spleen after the conventional preoperative regimen. Splenectomy is an option for ABO-LDLT non-responders.

  18. The locus for apolipoprotein E (apoE) is close to the Lutheran (Lu) blood group locus on chromosome 19.

    Science.gov (United States)

    Gedde-Dahl, T; Olaisen, B; Teisberg, P; Wilhelmy, M C; Mevåg, B; Helland, R

    1984-01-01

    Linkage has been described between the loci for apolipoprotein E (apoE) and the complement C3 (C3) on chromosome 19. C3 is known to belong to a linkage group with gene order C3-Se-Lu. The present study revealed linkage between Se and apoE with peak lod score +3.3 at recombination fraction 0.08 in males and +1.36 at 0.22 in females, and linkage between apoE and Lu with lod score +4.52 at zero recombination in sexes combined. The C3-apoE linkage gives lod score +4.00 at theta = 0.18 in males, but +0.04 at theta = 0.45 in females. Triple heterozygote families confirm that apoE is on the Se side and on the Lu side of C3. Allelic association between apoE and Lu has not been ruled out. Combining our data with published data on C3-Se and Se-Lu, this segment of chromosome 19 has an average age sex ratio of female/male recombination of 2.3.

  19. 军队不同年龄组男性干部血脂、血压、血糖检测结果比较%Comparison on detection results of blood lipid, blood pressure and blood glucose between different age groups in cadres of the army men

    Institute of Scientific and Technical Information of China (English)

    赵建武; 严鹏飞; 许峰; 赵惠娟

    2012-01-01

    目的 比较部队男性干部不同年龄组血脂、血压、血糖检测结果,找出差异,从而有针对性的做好健康教育.方法 将2005年5月-2011年5月在我院疗养的3026例男性干部根据年龄分为:老年前期组484例,老年组1785例,高龄组757例,对各组血脂、血压、血糖异常分布及影响因素进行分析.结果 老年前期组低高密度脂蛋白、高舒张压、饮酒、吸烟、缺乏运动的检出率分别为8.7%、23.8%、47.3%、28.1%、36.8%明显高于老年组的3.4%、16.2%、35.1%、18.1%、18.2%;而高收缩压、高空腹血糖、高餐后2h血糖及超重/肥胖的检出率明显降低,差异有统计学意义(P<0.01);高龄组高收缩压、缺乏运动的检出率分别为40.2%、27.3%明显高于老年组的32.6%、18.2%,高三酰甘油、高舒张压、高餐后2h血糖,超重/肥胖,饮酒、吸烟的检查率明显降低,差异有统计学意义(P<0.01).结论 军队男性干部老年前期组低HDL-C、高DBP检出率高于老年组、高年组SBP、缺乏运动检出率明显增高;老年前组饮酒、吸烟、缺乏运动检出率增高.%Objective To compare the detection results of blood lipid, blood pressure and blood glucose between different age groups in cadres of the army men and find out the difference in order to improve the effect of health education. Methods From May 2005 — May 2011, 3 026 cases of male cadre in our hospital were selected for the study. According to the ages, patients were divided into pre-elderly group (n = 484) , old age group ( n - 1 785 ) and very old age group ( n = 757). The abnormal distributions of blood lipid, blood pressure and blood glucose and influence factors were analyzed. Results The detection rates of low high-density lipoprotein, high diastolic blood pressure, drinking, smoking, lack of exercise in pre-elderly group were 8.7% , 23. 8% , 47.3% , 28. 1% and 36. 8% respectively, significantly higher than 3. 4

  20. PREVALENCE OF GIARDIA LAMBLIA AND OTHER INTESTINAL PARASITES IN PRESCHOOL CHILDREN AND ITS RELATION TO RESIDENCE PLACE, SEX AND BLOOD GROUP IN ILAM COUNTY OF IRAN

    Directory of Open Access Journals (Sweden)

    Pourbabak

    1996-06-01

    Full Text Available 1711' object of this study was to determine the prevalence oj asymptomatic infestation with Giardia lamblia lind other intestinal parasites in children of urban anti rural communities oj /lam county and its relation with dwelling place, sex and blood grollp!.. 77w study designed as (l five-month pUTasitoulgica! .m,..£!' oj fecal ami blood specimens from humans anti performed in 10 urban hcalih-trcatmcru clinics of llam city, two urban health treatment clinics of Eyvun city, two rural health-treatment clinics oj Chavar and Sartaf villages, llam province west of fran, 17,e examined population was preschool {, to 7 year-old children without any 'gastrointestinal compliarus. Prevalence oj infestation in subject grOllp W(l."' 32.54% (n=3100. Among intestinal parasites' G. lambliu with 85.43o/c (27.8% oj all, n=JO(JI prevalence rate was the most common. Infestation with 11. nnrm with 1'/.93% and E. coli with 3.07';, were in the second and third ranks, respectively, Infestation shows a distinct relationship with gender (P<0.05 and dwelling place, but it lacks a significant relation with blood groups. This study ."lIOWS that the prevalence of intestinal infestation in 6 to 7 year old child oj llam county hi equivalent to the top oj tile line oj the statistical percentage all over the world. 17,e relation between the severity oj infestation and residence place may arouse the suspicion oj sever contamination oj imbibing water.

  1. 中国人群ABO血型系统基因分型研究与应用%Genotyping of ABO blood group in Chinese Han population

    Institute of Scientific and Technical Information of China (English)

    喻琼; 吴国光; 梁延连; 苏宇清

    2005-01-01

    背景:ABO血型是输血医学中最重要的血型系统,血型血清学定型技术具有简单实用的特点已广泛应用于ABO血型中的鉴定中,但是血清学试验存在局限性,而基因技术在某种程度上克服了血清学试验的缺点.目的:研究中国人群ABO基因多态性,并将基因分型技术用于解决临床输注中血型血清学难题.设计:随机选取样品,与常规血型血清学结果相比较,分析基因型结果.单位:一所市级血液中心输血医学研究所.对象:选择2002-03/2002-12在深圳市血液中心参加捐血的无血缘关系的中国汉族无偿献血者个体260人为研究对象,其中男110人,女150人,年龄18~50岁.1例血清学技术正反定型不符的标本来自本血液中心并调查其家系,6例血清学疑为A2型的标本来自第二人民医院等4个本市医院输血科.方法:快速盐析法提取外周血中的DNA,采用聚合酶链反应-序列特异性引物基因方法对ABO血型定型,并且在吸收放散试验、唾液血型物质凝集抑制等血清学试验基础上,用聚合酶链反应-序列特异性引物法扩增ABO血型的等位基因.主要观察指标:260人份样品ABO血型系统的基因型及疑难血型样品的血清型与基因型.结果:在中国汉族符合Hardy-Weinberg平衡的随机群体(260人)中,检出O1,B,A1O1(A467C)和A1O2/1O3(A467T)4种等位基因,其基因频率分别为0.582 7,0.184 6,0.009 6,0.223 1.在疑难血型鉴定中6份血清学定为A2的标本中,只有2例基因分型确定为A2O1O1,其余均为A1O2/A1O3O1型,在一家系疑难血型鉴定中,兄弟3人均为类孟买型,ABO基因分型分别为A1O2B,A1O2B,A1O2O1.结论:ABO聚合酶链反应-序列特异性引物基因分型是一种方便、快速、可靠的技术,可以弥补血型血清学鉴定方法的不足.%BACKGROUND: ABO is the most important blood group system for blood transfusion. Though widely used in determining ABO blood group for its simplicity and

  2. Missense mutation of FUT1 and deletion of FUT2 are responsible for Indian Bombay phenotype of ABO blood group system.

    Science.gov (United States)

    Koda, Y; Soejima, M; Johnson, P H; Smart, E; Kimura, H

    1997-09-01

    The Bombay phenotype fails to express the ABH antigens of ABO blood group system on red blood cells and in secretions because of a lack in activities of the H gene (FUT1)- and Secretor gene (FUT2)-encoded alpha (1,2)fucosyltransferases. In this study, we have examined the FUT1 and the FUT2 from three unrelated Indian individuals with the Bombay phenotype. These three individuals were found to be homozygous for a T725G mutation in the coding region of the FUT1, which inactivated the enzyme activity. In addition, we did not detect any hybridized band corresponding to the FUT2 by Southern blot analysis using the catalytic domain of the FUT2 as a probe, indicating that the three individuals were homozygous for a gene deletion in the FUT2. These results suggest that the T725G mutation of FUT1 and the gene deletion of FUT2 are responsible for the classical Indian Bombay phenotype.

  3. Antigen-Specific versus Non-Antigen-Specific Immunoadsorption in ABO-Incompatible Renal Transplantation.

    Directory of Open Access Journals (Sweden)

    Gerold Thölking

    Full Text Available ABO-incompatible (ABOi renal transplantation (RTx from living donors is an established procedure to expand the donor pool for patients with end stage renal disease. Immunoadsorption (IA is a standard procedure for the removal of preformed antibodies against the allograft. In this study, antigen-specific and non-antigen-specific IA in ABOi RTx were compared.10 patients underwent antigen-specific IA (Glycosorb group and 13 patients non-antigen-specific IA (Immunosorba group. The effects of both procedures regarding antibody reduction, number of treatments, complications, costs, as well as the allograft function and patient survival were compared between both groups.Although the IgG levels were reduced equally by both procedures (p=0.82, the reduction of the IgM level was more effective in the Glycosorb group (p=0.0172. Patients in both groups required a median number of 6 IA before ABOi RTx. Allograft function at one year after AB0i RTx was similar in both groups (estimated glomerular filtration rate: 66 vs. 64 ml/min/1.73m² respectively, with a death-censored graft survival of 90.0% and 92.3% respectively. Complication rates did not differ between procedures. Due to the reuse of non-antigen-specific Immunosorba columns, costs were considerably lower in this group; however, the use of the Immunosorba-based IA was less time-efficient.Considering upcoming alternatives as simultaneous performance of dialysis and IA or a possible reuse of Glycosorb columns, this might become less relevant in the future.

  4. Risk factor for ischemic-type biliary lesion after ABO-incompatible living donor liver transplantation

    Science.gov (United States)

    Bang, Jun Bae; Kim, Bong-Wan; Kim, Young Bae; Wang, Hee-Jung; Lee, Hyun Yeong; Sim, Joohyun; Kim, Taegyu; Lee, Kyeong Lok; Hu, Xu-Guang; Mao, Wei

    2016-01-01

    AIM: To evaluate the risk factors for ischemic-type biliary lesion (ITBL) after ABO-incompatible (ABO-I) adult living donor liver transplantation (ALDLT). METHODS: Among 141 ALDLTs performed in our hospital between 2008 and 2014, 27 (19%) were ABO-I ALDLT and 114 were ABO-identical/compatible ALDLT. In this study, we extensively analyzed the clinico-pathological data of the 27 ABO-I recipients to determine the risk factors for ITBL after ABO-I ALDLT. All ABO-I ALDLT recipients underwent an identical B-cell depletion protocol with preoperative rituximab, plasma exchange (PE), and operative splenectomy. The median follow-up period after transplantation was 26 mo. The clinical outcomes of the 27 ABO-I ALDLT recipients were compared with those of 114 ABO-identical/compatible ALDLT recipients. RESULTS: ITBL occurred in four recipients (14.8%) between 45 and 112 d after ABO-I ALDLT. The overall survival rates were not different between ABO-I ALDLT and ABO-identical/compatible ALDLT (P = 0.303). Among the ABO-I ALDLT recipients, there was no difference between patients with ITBL and those without ITBL in terms of B-cell and T-cell count, serum isoagglutinin titers, number of PEs, operative time and transfusion, use of graft infusion therapy, or number of remnant B-cell follicles and plasma cells in the spleen. However, the perioperative NK cell counts in the blood of patients with ITBL were significantly higher than those in the patients without ITBL (P 150/μL and postoperative NK cell count > 120/μL were associated with greater relative risks (RR) for development of ITBL (RR = 20 and 14.3, respectively, P transplant recipient’s blood are associated with ITBL after ABO-I ALDLT. Further research is needed to elucidate the molecular mechanism of NK cell involvement in the development of ITBL.

  5. When Compatibility Interferes with Group Effectiveness: Facilitation of Learning in Small Groups.

    Science.gov (United States)

    Shaw, Marvin, E.; Webb, Jeaninne N.

    1982-01-01

    Examined whether compatible groups facilitate learning more than incompatible groups. Used peer groups to facilitate learning in college courses. Computed compatibility scores for pairs and four-person groups working together. Used examinations to measure peer group procedure effectiveness. Results did not indicate a positive relationship between…

  6. Genetic incompatibility dampens hybrid fertility more than hybrid viability: yeast as a case study.

    Directory of Open Access Journals (Sweden)

    Meibo Xu

    Full Text Available Genetic incompatibility is believed to be the major cause of postzygotic reproductive isolation. Despite huge efforts seeking for speciation-related incompatibilities in the past several decades, a general understanding of how genetic incompatibility evolves in affecting hybrid fitness is not available, primarily due to the fact that the number of known incompatibilities is small. Instead of further mapping specific incompatible genes, in this paper we aimed to know the overall effects of incompatibility on fertility and viability, the two aspects of fitness, by examining 89 gametes produced by yeast S. cerevisiae-S. paradoxus F1 hybrids. Homozygous F2 hybrids formed by autodiploidization of F1 gametes were subject to tests for growth rate and sporulation efficiency. We observed much stronger defects in sporulation than in clonal growth for every single F2 hybrid strain, indicating that genetic incompatibility affects hybrid fertility more than hybrid viability in yeast. We related this finding in part to the fast-evolving nature of meiosis-related genes, and proposed that the generally low expression levels of these genes might be a cause of the observation.

  7. Genetic diversity and fitness in small populations of partially asexual, self-incompatible plants.

    Science.gov (United States)

    Navascués, M; Stoeckel, S; Mariette, S

    2010-05-01

    How self-incompatibility systems are maintained in plant populations is still a debated issue. Theoretical models predict that self-incompatibility systems break down according to the intensity of inbreeding depression and number of S-alleles. Other studies have explored the function of asexual reproduction in the maintenance of self-incompatibility. However, the population genetics of partially asexual, self-incompatible populations are poorly understood and previous studies have failed to consider all possible effects of asexual reproduction or could only speculate on those effects. In this study, we investigated how partial asexuality may affect genetic diversity at the S-locus and fitness in small self-incompatible populations. A genetic model including an S-locus and a viability locus was developed to perform forward simulations of the evolution of populations of various sizes. Drift combined with partial asexuality produced a decrease in the number of alleles at the S-locus. In addition, an excess of heterozygotes was present in the population, causing an increase in mutation load. This heterozygote excess was enhanced by the self-incompatibility system in small populations. In addition, in highly asexual populations, individuals produced asexually had some fitness advantages over individuals produced sexually, because sexual reproduction produces homozygotes of the deleterious allele, contrary to asexual reproduction. Our results suggest that future research on the function of asexuality for the maintenance of self-incompatibility will need to (1) account for whole-genome fitness (mutation load generated by asexuality, self-incompatibility and drift) and (2) acknowledge that the maintenance of self-incompatibility may not be independent of the maintenance of sex itself.

  8. 46 CFR 150.130 - Loading a cargo on vessels carrying cargoes with which it is incompatible.

    Science.gov (United States)

    2010-10-01

    ... it is incompatible. 150.130 Section 150.130 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... carrying cargoes with which it is incompatible. Except as described in § 150.160, the person in charge of a... any cargo in table I with which it is incompatible by two barriers such as formed by a: (1)...

  9. Blood Clots

    Science.gov (United States)

    ... Index A-Z Blood Clots Blood clots are semi-solid masses of blood that can be stationary (thrombosis) ... treated? What are blood clots? Blood clots are semi-solid masses of blood. Normally, blood flows freely through ...

  10. 广西壮族人群稀有血型筛选%Screening of the rare blood group in Zhuang population of Guangxi province

    Institute of Scientific and Technical Information of China (English)

    焦伟; 黄梅会; 朱自严; 黎海澜; 王晨; 伍焕秀; 莫柱宁; 阳子骥; 蓝娇; 刘斐; 肖瑞平

    2011-01-01

    To discover the frequency and distribution of the blood group of red blood cells Ena-, Lub- Ge-, Jk(a-b-) in Zhuang population, provide the evidence of checking rational spectrum cells which are established to meet local distribution pattern of blood group antigens before clinical transfusion. Mur+, H- were screened by immediately centrifugal tube method; DCE/ DCE.. DCE/dCE, dCE/dCE phenotype were screened by indirect antiglobulin test or 96 microplate directly centrifugal method; Lub-, Ena-, Ge-, Wrb- and I were screened by 96 microplate directly centrifugal method and indirect antiglobulin test; JK(a-b-) phenotype was screened by 2 mol/L urea hemolysis test. 57 Lub- and 2 Jk(a-b-) were screened in 4 527 Zhuang population; 319 Mur-r- were screened in 2 825 which was a part of 4 527 Zhuang population. None of Ena-, Ge-, Wrb-, Bombay blood group, adult I, CDE/CDE and CdE/CdE and dCE/dCE phenotype was found. This result demonstrates that the rare phenotype fre-quency(0. 044%) of Jk(a-b-) in Guangxi Zhuang population is higher than in European Caucasian, Chinese Shanghai and Guangdong Panyu population. The phenotype frequency of Lub- is 1. 26% , the frequency of Mu+(11. 29%) is higher than in Shanghai and Guangdong Panyu, but is apparently lower than in Yunnan Nu population.%了解广西壮族人群中Ena-、Lub-、Ge-、Jk(a-b-)红细胞血型的频率与分布情况,为建立符合本地区血型抗原分布格局合理的谱细胞进行临床输血前检查提供依据.采用立即离心试管法筛选Mur+,H-;用试管法间接抗球蛋白试验或96孔微量板法直接离心试验筛选DCE/DCE、DCE/dCE、dCE/dCE表型;96孔微量板法直接离心试验和间接抗球蛋白试验筛选Lub-、Ena-、Ge-,Wrb-和i;2 mol/L尿素溶血试验筛选JK(a-b-)表型.在4 527名壮族人群中筛选出Lub-57例、Jk(a-b-)2例;对其中2 825例壮族筛选出Mur+319例,没有发现Ena-、Ge-,Wrb-、类孟买血型、成人i及DCE/DCE,DCE/dCE,dCE/dCE表型.结果显示,广

  11. Interspecific crossing and genetic mapping reveal intrinsic genomic incompatibility between two Senecio species that form a hybrid zone on Mount Etna, Sicily.

    Science.gov (United States)

    Brennan, A C; Hiscock, S J; Abbott, R J

    2014-09-01

    Studies of hybridizing species can reveal much about the genetic basis and maintenance of species divergence in the face of gene flow. Here we report a genetic segregation and linkage analysis conducted on F2 progeny of a reciprocal cross between Senecio aethnensis and S. chrysanthemifolius that form a hybrid zone on Mount Etna, Sicily, aimed at determining the genetic basis of intrinsic hybrid barriers between them. Significant transmission ratio distortion (TRD) was detected at 34 (∼27%) of 127 marker loci located in nine distinct clusters across seven of the ten linkage groups detected, indicating genomic incompatibility between the species. TRD at these loci could not be attributed entirely to post-zygotic selective loss of F2 individuals that failed to germinate or flower (16.7%). At four loci tests indicated that pre-zygotic events, such as meiotic drive in F1 parents or gametophytic selection, contributed to TRD. Additional tests revealed that cytonuclear incompatibility contributed to TRD at five loci, Bateson-Dobzhansky-Muller (BDM) incompatibilities involving epistatic interactions between loci contributed to TRD at four loci, and underdominance (heterozygote disadvantage) was a possible cause of TRD at one locus. Major chromosomal rearrangements were probably not a cause of interspecific incompatibility at the scale that could be examined with current map marker density. Intrinsic genomic incompatibility between S. aethnensis and S. chrysanthemifolius revealed by TRD across multiple genomic regions in early-generation hybrids is likely to impact the genetic structure of the natural hybrid zone on Mount Etna by limiting introgression and promoting divergence across the genome. PMID:24595365

  12. Flowering time and incompatibility groups: Cultivar combination in commercial sweet cherry (Prunus avium L.) orchards

    OpenAIRE

    Radičević Sanja; Cerović Radosav; Marić Slađana; Đorđević Milena

    2011-01-01

    The paper presents results of eight-year study (1999-2006) of flowering phenophase in 21 introduced sweet cherry cultivars grown under the agro-environmental conditions of West Serbia. Flowering time, as well as progress and abundance of flowering were studied, and classification of the studied cultivars according to flowering time was derived. On the basis of mean several-year overlap in phenophase of full flowering and on the grounds of so far known data ...

  13. Arrow physicians: are economics and medicine philosophically incompatible?

    Science.gov (United States)

    Tsang, Sandro

    2015-06-01

    Economics is en route to its further expansion in medicine, but many in the medical community remain unconvinced that its impact will be positive. Thus, a philosophical enquiry into the compatibility of economics and medicine is necessary to resolve the disagreements. The fundamental mission of medicine obliges physicians to practise science and compassion to serve the patient's best interests. Conventional (neoclassical) economics assumes that individuals are self-interested and that competitive markets will emerge optimal states. Economics is seemingly incompatible with the emphasis of putting patients' interests first. This idea is refuted by Professor Kenneth Arrow's health economics seminal paper. Arrow emphasizes that medical practice involves agency, knowledge, trust and professionalism, and physician-patient relation critically affects care quality. The term Arrow Physician is used to mean a humanistic carer who has a concern for the patient and acts on the best available evidence with health equity in mind. To make this practice sustainable, implementing appropriate motivations, constitutions and institutions to enable altruistic agency is critical. There is substantial evidence that polycentric governance can encourage building trust and reciprocity, so as to avoid depletion of communal resources. This paper proposes building trusting institutions through granting altruistic physicians adequate autonomy to direct resources based on patients' technical needs. It also summarizes the philosophy bases of medicine and economics. It, therefore, contributes to developing a shared language to facilitate intellectual dialogues, and will encourage trans-disciplinary research into medical practice. This should lead to medicine being reoriented to care for whole persons again. PMID:25850973

  14. A Method for Prediction of Graft Incompatibility in Sweet Cherry

    Directory of Open Access Journals (Sweden)

    S. Filiz GUCLU

    2012-05-01

    Full Text Available This study was carried out on 1-year old trees of ‘0900 Ziraat’ variety grafted onto ‘Kuş kirazi’, ‘Kara idris’, ‘Sari idris’, ‘MaxMa 14’, ‘MaxMa 60’ and ‘Gisela 5’ in order to determine their compatibility. For this purpose, total peroxidase activity was determined by spectrophotomery assay. Taking ground tissue samples were planned three times as before the grafting (beginning, and then 8 and 12 months after grafting. Total peroxidase activities of rootstocks before grafting varied between 10.80 ΔAg.min. (‘Kuş kirazi’ and 7.83 ΔAg.min. (‘Kara idris’ and were found to be statistically important. The peroxidase activity of ‘0900 Ziraat’ was 11.07ΔAg.min. and the closest value occurred in Prunus avium rootstock. The most different values occurred in rootstocks of ‘Kara idris’ and ‘Gisela 5’. The results showed that peroxidase activity increased in rootstock and graft scion. This increase had higher values in heterogenetic combinations especially in ‘0900 Ziraat’/‘Gisela 5’ and ‘0900 Ziraat’/‘Kara idris’ grafts. Peroxidase activity was decreased at the 12th month for all combinations. The highest value was obtained from ‘0900 Ziraat’/‘MaxMa 14’ combinaion with 29.17 ΔAg.min. while lowest one was ‘0900 Ziraat’/‘Kuş kirazi’ with 17.39. The findings showed that peroxidase activity could be used as a parameter in early determination of graft incompatibility.

  15. Distribution of ABO blood group and the clinical characteristics in gastric cancer%胃癌患者的ABO血型分布及临床特点分析

    Institute of Scientific and Technical Information of China (English)

    徐灵均; 张吉翔

    2012-01-01

    目的 探讨胃癌患者的ABO血型分布及临床特点.方法 选择400例胃癌患者的住院病历资料,分析其ABO血型;比较前后5年患者的ABO血型分布,分析后5年患者的一般情况、首发症状、病理特点.结果 与前5年比较,后5年A型血检出率明显减少(41.5% vs 23.5%,P<0.01),O型血检出率明显增多(27.5% vs 42.5%,P<0.01),B型、AB型血无统计学差异.后5年中,各血型患者的首发症状均以上腹痛、腹胀、纳差、体质量下降为主;B型血低分化腺癌患者明显高于A型血患者(68.1% vs 36.1%,P<0.05),70.7%浸润深度达全层,且66.4%有淋巴结转移;累及周围组织的AB型血患者明显高于B型血患者(61.5%vs 25.5%,P<0.05).结论 前后5年胃癌患者的ABO血型分布发生了变化;提示非遗传因素对胃癌发病的影响逐渐增强,血型可能与肿瘤的生物学行为有关.%Objective To explore the distribution of ABO blood group and the clinical features of the patients with gastric cancer (GC). Methods Inpatient medical records of four hundred cases of the patients with CC were choosed and put into statistical analysis of ABO blood group; To compare the distribution of ABO blood group of the patients before and after five years, at the same time analyzing the clinical data of the patients included the generalconditions, clinical manifestation, pathological features, auxiliary examination, staging, treat ment and so on. Results Compared with the prior five years, the detectable rate of A blood group was greatly decreased in the recent five years (41.5% vs 23.5% , P <0.01) , the detectable rate of 0 blood group was obviously increased in the recent five years (27.5% vs 42.5% , P <0.01), the discrepancy of B blood group and AB blood group were not statistically significant. In the recent five years, initial symptoms in each blood type group were mainly upper abdominal pain or distention, anorexia, marasmus; The rates of poorly

  16. The joint evolution and maintenance of self-incompatibility with gynodioecy or androdioecy.

    Science.gov (United States)

    Van de Paer, Céline; Saumitou-Laprade, Pierre; Vernet, Philippe; Billiard, Sylvain

    2015-04-21

    Mating systems show two kinds of frequent transitions: from hermaphroditism to dioecy, gynodioecy or androdioecy, or from self-incompatibility (SI) to self-compatibility (SC). While models have mostly investigated these two kinds of transitions as independent, empirical observations suggest that, to some extent, they can evolve jointly. Here, we study the joint evolution and maintenance of SI and androdioecy or SI and gynodioecy by the means of phenotypic models. Our models focus on three parameters: the unisexuals׳ advantage relative to that of the hermaphrodites due to resource reallocation, inbreeding depression and the selfing rate. We assume no pollen limitation or discounting. We show that SI helps the maintenance of androdioecy, but favors the loss of gynodioecy, and also that androdioecy facilitates the maintenance of SI, whereas gynodioecy does not affect it. We finally investigate how gynodioecy and androdioecy may affect the diversification of SI groups, especially considering an evolutionary pathway through SC intermediates. We show that while androdioecy prevents the increase of the number of SI groups, under certain conditions of inbreeding depression and selfing rates, gynodioecy allows it.

  17. Expression, purification and X-ray crystallographic analysis of the Helicobacter pylori blood group antigen-binding adhesin BabA.

    Science.gov (United States)

    Subedi, Suresh; Moonens, Kristof; Romão, Ema; Lo, Alvin; Vandenbussche, Guy; Bugaytsova, Jeanna; Muyldermans, Serge; Borén, Thomas; Remaut, Han

    2014-12-01

    Helicobacter pylori is a human pathogen that colonizes about 50% of the world's population, causing chronic gastritis, duodenal ulcers and even gastric cancer. A steady emergence of multiple antibiotic resistant strains poses an important public health threat and there is an urgent requirement for alternative therapeutics. The blood group antigen-binding adhesin BabA mediates the intimate attachment to the host mucosa and forms a major candidate for novel vaccine and drug development. Here, the recombinant expression and crystallization of a soluble BabA truncation (BabA(25-460)) corresponding to the predicted extracellular adhesin domain of the protein are reported. X-ray diffraction data for nanobody-stabilized BabA(25-460) were collected to 2.25 Å resolution from a crystal that belonged to space group P21, with unit-cell parameters a = 50.96, b = 131.41, c = 123.40 Å, α = 90.0, β = 94.8, γ = 90.0°, and which was predicted to contain two BabA(25-460)-nanobody complexes per asymmetric unit. PMID:25484214

  18. Effect of Broadcasting Gymnastics on Blood Lipid in Different Age Groups%广播体操对不同年龄人群血脂的影响

    Institute of Scientific and Technical Information of China (English)

    朱小宁; 薛芹波; 金丽

    2014-01-01

    通过问卷调查筛选出武汉市138名无遗传病史的健康中年人和非体育专业的大学生,按年龄随机分成对照组和实验组。对实验组进行为期3个月的广播体操干预,每周至少做3次,每次做6套,每套间歇1min。两组受试者在实验期间均保持正常健康饮食。受试者于实验开始前和结束后的两个早晨,在安静状态下空腹抽取6ml的静脉血,测定实验前后总胆固醇、甘油三酯、低密度脂蛋白和高密度脂蛋白的值。结果显示:3个月的广播体操能有效改善中年人的血脂水平,尤以中年男性的低密度脂蛋白含量改善效果最为明显;对青年人的血脂水平也有相应的改善效果,但相比之下较不明显。%This paper choses 138 healthy middle-aged people without genetic diseases history and non-professional sports college students in Wuhan by questionnaire,and randomly divided them into control group and experimental groups according to the age. The subjects of experimental group did broadcasting gymnastics for at least three times a week,every time do six sets,each batch for one minute. Both subjects of two groups have a normal healthy diet during the experiment. All Subjects were extracted 6ml of venous blood in the morning two days before and after they were in the experiment, in quiet state and on empty stomach,measuring their blood lipid four indicators before and after the experiment(total cholesterol,triglyceride, low density lipoprotein cholesterol and high-density lipoprotein)values. We eliminated invalid samples during the experimental process. Results showed that 3 months of broadcasting gymnastics can effectively improve middle-aged people's blood lipid levels, especially in the middle-aged men's low density lipoprotein level and it also has a comparative improvement to the youngsters' lipids level, though not so obvious contrasting with their counterpart.

  19. 探讨ABO血型梯形微型板在数字血型仪中的应用%Application of ABO blood group of trapezoid micro plate in adigital type instrument

    Institute of Scientific and Technical Information of China (English)

    刘涛; 桑仁贵; 李茂恩

    2014-01-01

    Objective:The evaluation trapezoid micro-plate method to detect ABO blood group.Method:use auto-matic sampling system and Blood Group's apparetus to analyse specimen's blood group.Result:The detection of the blood group of 9619 blood donors anti-coagulation specimens, were 99.7% correct.Conclusion:The trapezoid mi-cro-plate method is simple, accurate, high sensitivity,routine detection of blood type is applicable to bulk sample.%目的:评价梯形微型板在血型检测中的应用。方法:全自动加样系统加样,血型判读仪进行判读。结果:通过对9619例无偿献血者血液抗凝标本的血型检测,正确率99.7%。结论:梯形微型板方法操作简便,结果准确,灵敏度高,适用于大批量样本的血型常规检测。

  20. Linkage disequilibrium between incompatibility locus region genes in the plant Arabidopsis lyrata

    DEFF Research Database (Denmark)

    Hagenblad, Jenny; Bechsgaard, Jesper Smærup; Charlesworth, Deborah

    2006-01-01

    We have studied diversity in Arabidopsis lyrata of sequences orthologous to the ARK3 gene of A. thaliana. Our main goal was to test for recombination in the S-locus region. In A. thaliana, the single-copy ARK3 gene is closely linked to the non-functional copies of the self-incompatibility loci, and...... the ortholog in A. lyrata (a self-incompatible species) is in the homologous genome region and is known as Aly8. It is thus of interest to test whether Aly8 sequence diversity is elevated due to close linkage to the highly polymorphic incompatibility locus, as is theoretically predicted. However, Aly8...... linked to the incompatibility locus, one being a pseudogene. We determined the phase of multiple haplotypes in families of plants from Icelandic and other populations. Different Aly8 sequence types are associated with different SRK alleles, while haplotypes with the same SRK sequences tend to have the...

  1. Expression of self-incompatibility ribonucleases of Antirrhinum in Escherichia coli

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Self-incompatibility is an intraspecific reproductive barrier to prevent self-fertilization in the flowering plants.In many species,self-incompatibility is controlled by a single S locus with multiple alleles.So far,the only gene known in the S locus of the Solanaceae,Scrophulariaceae and Rosaceae encodes a class of ribonucleases,called self-incompatibility ribonucleases (S RNases),which have been shown to mediate stylar expression of self-incompatible reaction.As the first step to investigate their three-dimensional structure,we successfully expressed three biologically active S RNases of Antirrihnum (S2,S4 and S5) in Escherichia coli (E.coli).Their functional expressions caused no detrimental effect on host bacteria growth and provided a basis for a large scale preparation of S RNase proteins.Possible reasons for non-lethality of S RNases on E.coli are discussed.

  2. Against the Quantitative-Qualitative Incompatibility Thesis or Dogmas Die Hard.

    Science.gov (United States)

    Howe, Kenneth R.

    1988-01-01

    Employs a pragmatic philosophical perspective to argue that there is no incompatibility at either the level of practice or that of epistemology between qualitative and quantitative methods of educational research. (FMW)

  3. European Group for Blood and Marrow Transplantation Centers with FACT-JACIE Accreditation Have Significantly Better Compliance with Related Donor Care Standards.

    Science.gov (United States)

    Anthias, Chloe; O'Donnell, Paul V; Kiefer, Deidre M; Yared, Jean; Norkin, Maxim; Anderlini, Paolo; Savani, Bipin N; Diaz, Miguel A; Bitan, Menachem; Halter, Joerg P; Logan, Brent R; Switzer, Galen E; Pulsipher, Michael A; Confer, Dennis L; Shaw, Bronwen E

    2016-03-01

    Previous studies have identified healthcare practices that may place undue pressure on related donors (RDs) of hematopoietic cell products and an increase in serious adverse events associated with morbidities in this population. As a result, specific requirements to safeguard RD health have been introduced to Foundation for the Accreditation of Cellular Therapy/The Joint Accreditation Committee ISCT and EBMT (FACT-JACIE) Standards, but the impact of accreditation on RD care has not previously been evaluated. A survey of transplant program directors of European Group for Blood and Marrow Transplantation member centers was conducted by the Donor Health and Safety Working Committee of the Center for International Blood and Marrow Transplant Research to test the hypothesis that RD care in FACT-JACIE accredited centers is more closely aligned with international consensus donor care recommendations than RD care delivered in centers without accreditation. Responses were received from 39% of 304 centers. Our results show that practice in accredited centers was much closer to recommended standards as compared with nonaccredited centers. Specifically, a higher percentage of accredited centers use eligibility criteria to assess RDs (93% versus 78%; P = .02), and a lower percentage have a single physician simultaneously responsible for an RD and their recipient (14% versus 35%; P = .008). In contrast, where regulatory standards do not exist, both accredited and nonaccredited centers fell short of accepted best practice. These results raise concerns that despite improvements in care, current practice can place undue pressure on donors and may increase the risk of donation-associated adverse events. We recommend measures to address these issues through enhancement of regulatory standards as well as national initiatives to standardize RD care.

  4. Incompatibilidad gametofítica en las razas mexicanas de maíz Gametophytic incompatibility in Mexican maize breeds

    Directory of Open Access Journals (Sweden)

    José Miguel Padilla García

    2012-06-01

    was to detect alleles of cross-incompatibility of ga1 and tcb1 in collections representing maize breeds groups from Mexico. The work was made at Centro Universitario de Ciencias Biológicas y Agropecuarias from Universidad de Guadalajara, located in Zapopan, Jalisco, Mexico during agricultural spring-summer 2007 to 2009 cycles. Under rainfall conditions 184 accessions of Mexican maize breeds were assessed based in percentage of grain filling in ear in their crosses with recessive male testers (without incompatibility factors and dominant female testers (with incompatibility factors. The analysis of variance suggested highly significant differences as well between breeds as between accessions within breeds for locus ga1. With regards locus tcb1 there were found only highly significant differences between breeds. Allele Ga1-S, responsible of non-reciprocal incompatibility, was detected in 53.8% of accessions, while neutral allele Ga1-m and recessive allele ga1 was identified in 55.43 and 15.76%, respectively. Allele Tcb1-S was detected at very low rates, mainly found in breeds Elotes-Occidentales and Maíz-Dulce.

  5. Expression and inheritance of sporophytic self-incompatibility in synthetic allohexaploid Senecio cambrensis (Asteraceae).

    Science.gov (United States)

    Brennan, Adrian C; Hiscock, Simon J

    2010-04-01

    Allopolyploid speciation is common in plants and is frequently associated with shifts from outcrossing, for example self-incompatibility, to inbreeding (i.e. selfing). Senecio cambrensis is a recently evolved allohexaploid species that formed following hybridization between diploid self-incompatible S. squalidus and tetraploid self-compatible S. vulgaris. Studies of reproduction in wild populations of S. cambrensis have concluded that it is self-compatible. Here, we investigated self-compatibility in synthetic lines of S. cambrensis generated via hybridization and colchicine-induced polyploidization and wild S. cambrensis using controlled crossing experiments. Synthetic F(1)S. cambrensis individuals were all self-compatible but, in F(2) and later generations, self-incompatible individuals were identified at frequencies of 6.7-9.2%. Self-incompatibility was also detected in wild sampled individuals at a frequency of 12.2%. The mechanism and genetics of self-incompatibility were tested in synthetic S. cambrensis and found to be similar to those of its paternal parent S. squalidus (i.e. sporophytic). These results show, for the first time, that functional sporophytic self-incompatibility can be inherited and expressed in allopolyploids as early as the second (F(2)) generation. Wild S. cambrensis should therefore be considered as possessing a mixed mating system with the potential for evolution towards either inbreeding or outcrossing. PMID:19895670

  6. Contrasting levels of variability between cytoplasmic genomes and incompatibility types in the mosquito Culex pipiens.

    Science.gov (United States)

    Guillemaud, T; Pasteur, N; Rousset, F

    1997-02-22

    Reproductive incompatibilities called cytoplasmic incompatibilities are known to affect a large number of arthropod species and are mediated by Wolbachia, a maternally transmitted microorganism. The crossing relationships between strains of potential hosts define their incompatibility types and it is generally assumed that differences between strains of Wolbachia induce different crossing types. Among all the described host species, the mosquito, Culex pipiens, displays the greatest variability of cytoplasmic incompatibility crossing types. We analysed mitochondrial and bacterial DNA variability in Culex pipiens in order to investigate some possible causes of incompatibility crossing type variability. We sequenced fragments of the ftsZ gene, and the A + T-rich control region of the mtDNA. We also sequenced the second subunit of the mitochondrial cytochrome oxidase (COII) gene, in Culex pipiens and a closely related species, C. torrentium, in order to verify the usefulness of the A + T-rich region for the present purposes. No variability was found in the Wolbachia ftsZ gene fragment, and very limited variation of the mitochondrial marker whatever the compatibility type or the origin of the host. A low variability was found in the A + T-rich region and comparison of divergence of the A + T-rich region and COII gene between C. pipiens and C. torrentium did not reveal any special constraints affecting this region. In contrast to observations in other host species, variability of incompatibility crossing types is not due to multiple infections by distantly related Wolbachia strains. PMID:9061971

  7. Strategic transformation of population studies: recommendations of the working group on epidemiology and population sciences from the National Heart, Lung, and Blood Advisory Council and Board of External Experts.

    Science.gov (United States)

    Roger, Véronique L; Boerwinkle, Eric; Crapo, James D; Douglas, Pamela S; Epstein, Jonathan A; Granger, Christopher B; Greenland, Philip; Kohane, Isaac; Psaty, Bruce M

    2015-03-15

    In 2013, the National Heart, Lung, and Blood Institute assembled a working group on epidemiology and population sciences from its Advisory Council and Board of External Experts. The working group was charged with making recommendations to the National Heart, Lung, and Blood Advisory Council about how the National Heart, Lung, and Blood Institute could take advantage of new scientific opportunities and delineate future directions for the epidemiology of heart, lung, blood, and sleep diseases. Seven actionable recommendations were proposed for consideration. The themes included 1) defining the compelling scientific questions and challenges in population sciences and epidemiology of heart, lung, blood, and sleep diseases; 2) developing methods and training mechanisms to integrate "big data" science into the practice of epidemiology; 3) creating a cohort consortium and inventory of major studies to optimize the efficient use of data and specimens; and 4) fostering a more open, competitive approach to evaluating large-scale longitudinal epidemiology and population studies. By building on the track record of success of the heart, lung, blood, and sleep cohorts to leverage new data science opportunities and encourage broad research and training partnerships, these recommendations lay a strong foundation for the transformation of heart, lung, blood, and sleep epidemiology. PMID:25743324

  8. Self-incompatibility systems: barriers to self-fertilization in flowering plants.

    Science.gov (United States)

    Rea, Anne C; Nasrallah, June B

    2008-01-01

    Flowering plants (angiosperms) are the most prevalent and evolutionarily advanced group of plants. Success of these plants is owed to several unique evolutionary adaptations that aid in reproduction: the flower, the closed carpel, double fertilization, and the ultimate products of fertilization, seeds enclosed in the fruit. Angiosperms exhibit a vast array of reproductive strategies, including both asexual and sexual, the latter of which includes both self-fertilization and cross-fertilization. Asexual reproduction and self-fertilization are important reproductive strategies in a variety of situations, such as when mates are scarce or when the environment remains relatively stable. However, reproductive strategies promoting cross-fertilization are critical to angiosperm success, since they contribute to the creation of genetically diverse populations, which increase the probability that at least one individual in a population will survive given changing environmental conditions. The evolution of several physical and genetic barriers to self-fertilization or fertilization among closely related individuals is thus widespread in angiosperms. A major genetic barrier to self-fertilization is self-incompatibility (SI), which allows female reproductive cells to discriminate between "self" and "non-self" pollen, and specifically reject self pollen. Evidence for the importance of SI in angiosperm evolution lies in the highly diverse set of mechanisms used by various angiosperm families for recognition of self pollen tube development and preventing self-fertilization.

  9. Conditions for the Emergence of Shared Norms in Populations with Incompatible Preferences

    Science.gov (United States)

    Helbing, Dirk; Yu, Wenjian; Opp, Karl-Dieter; Rauhut, Heiko

    2014-01-01

    Understanding norms is a key challenge in sociology. Nevertheless, there is a lack of dynamical models explaining how one of several possible behaviors is established as a norm and under what conditions. Analysing an agent-based model, we identify interesting parameter dependencies that imply when two behaviors will coexist or when a shared norm will emerge in a heterogeneous society, where different populations have incompatible preferences. Our model highlights the importance of randomness, spatial interactions, non-linear dynamics, and self-organization. It can also explain the emergence of unpopular norms that do not maximize the collective benefit. Furthermore, we compare behavior-based with preference-based punishment and find interesting results concerning hypocritical punishment. Strikingly, pressuring others to perform the same public behavior as oneself is more effective in promoting norms than pressuring others to meet one’s own private preference. Finally, we show that adaptive group pressure exerted by randomly occuring, local majorities may create norms under conditions where different behaviors would normally coexist. PMID:25166137

  10. [Discovery of a novel A2 allel in ABO blood group system and investigation of its distribution in Han population of Chinese Fujian province].

    Science.gov (United States)

    Zhang, Ai; Chi, Quan; Ren, Ben-Chun

    2012-10-01

    This study was aimed to investigate the distribution of A2 subgroup in Han Population of Chinese Fujian province and its molecular mechanisms. One individual with serologic ABO blood grouping discrepancy was identified with commercially available monoclonal and polyclonal antibodies and lectin: anti-A, anti-B, anti-AB, anti-A1, and anti-H reagents according to the routine laboratory methods. DNA sequences of exon 6, 7 and intron 6 of ABO gene were analyzed by polymerase chain reaction using genomic DNA and direct DNA sequencing or sequencing after gene cloning. Red cells of 3 176 A or AB unrelated individuals were tested with anti-A1. The results showed that this individual was identified as A2 subgroup by serological technology, sequencing analysis indicated the A2 subgroup with novel A variant allele, the novel A allele being different from the allele A101 by 467C > T and 607G > A missense mutation in exon 7, no A2 subgroup was identified from the 3 176 individuals by using standard serological technology. It is concluded that a novel A allele responsible for A2 subgroup composing of 467C > T and 607G > A has been firstly confirmed, and the A2 subgroup is very rare in Chinese Fujian Han population.

  11. Novel association of ABO histo-blood group antigen with soluble ICAM-1: results of a genome-wide association study of 6,578 women.

    Directory of Open Access Journals (Sweden)

    Guillaume Paré

    2008-07-01

    Full Text Available While circulating levels of soluble Intercellular Adhesion Molecule 1 (sICAM-1 have been associated with diverse conditions including myocardial infarction, stroke, malaria, and diabetes, comprehensive analysis of the common genetic determinants of sICAM-1 is not available. In a genome-wide association study conducted among 6,578 participants in the Women's Genome Health Study, we find that three SNPs at the ICAM1 (19p13.2 locus (rs1799969, rs5498 and rs281437 are non-redundantly associated with plasma sICAM-1 concentrations at a genome-wide significance level (P<5x10(-8, thus extending prior results from linkage and candidate gene studies. We also find that a single SNP (rs507666, P = 5.1x10(-29 at the ABO (9q34.2 locus is highly correlated with sICAM-1 concentrations. The novel association at the ABO locus provides evidence for a previously unknown regulatory role of histo-blood group antigens in inflammatory adhesion processes.

  12. The Laminin 511/521 Binding Site on the Lutheran Blood Group Glycoprotein is Located at theFlexible Junction of Ig Domains 2 and 3

    Energy Technology Data Exchange (ETDEWEB)

    Mankelow, Tosti J.; Burton, Nicholas; Stedansdottir, Fanney O.; Spring, Frances A.; Parsons, Stephen F.; Pesersen, Jan S.; Oliveira, Cristiano L.P.; Lammie, Donna; Wess, Timothy; Mohandas, Narla; Chasis, Joel A.; Brady, R. Leo; Anstee, David J.

    2007-07-01

    The Lutheran blood group glycoprotein, first discovered on erythrocytes, is widely expressed in human tissues. It is a ligand for the {alpha}5 subunit of Laminin 511/521, an extracellular matrix protein. This interaction may contribute to vasocclusive events that are an important cause of morbidity in sickle cell disease. Using X-ray crystallography, small angle X-ray scattering and site directed mutagenesis we show that the extracellular region of Lutheran forms an extended structure with a distinctive bend between the second and third immunoglobulin-like domains. The linker between domains 2 and 3 appears to be flexible and is a critical determinant in maintaining an overall conformation for Lutheran that is capable of binding to Laminin. Mutagenesis studies indicate that Asp312 of Lutheran and the surrounding cluster of negatively charged residues in this linker region form the Laminin binding site. Unusually, receptor binding is therefore not a function of the domains expected to be furthermost from the plasma membrane. These studies imply that structural flexibility of Lutheran may be essential for its interaction with Laminin and present a novel opportunity for the development of therapeutics for sickle cell disease.

  13. 中药“十八反”基层医院应用现状调查%Research on Application Status of Eighteen Incompatible Medicaments in Primary Hospital

    Institute of Scientific and Technical Information of China (English)

    于大猛; 瞿融; 范欣生; 杨杰

    2012-01-01

    To investigate application status of eighteen incompatible medicaments in primary hospital. We searched 78 ,238 recipes originated from a county hospital of traditional Chinese medicine and 9 health clinics in towns and townships between 2008 and 2011. All recipes containing eighteen incompatible medicaments were statistically analyzed. Two handred and fifty-five recipes containing eighteen incompatible medicaments were found. There were 198 recipes in aconite group and 57 recipes in radix glycyrrhizae group. No recipe in veratrinigri group was found. Radix Aooniti Lateralis preparata in combination with Rhizoma Pinelliae was the most common formula of all eighteen incompatible medicaments. Most clinicians hold a negative attitude to application of eighteen incompatible medicaments. Application of eighteen incompatible medicaments in primary hospital is objectively existent, and it is not absolutely contraindicated.%调查基层医院应用中药十八反的现状.检索某县级中医院及9所乡镇卫生院2008-2011年的处方78 238首,对其中含十八反药对的处方进行统计分析.结果表明共发现含十八反药对的处方255首,其中乌头组198首,甘草组57首,藜芦组0首,以附子半夏应用最多,并出现多组反药同用的情况,临床医生对十八反问题多持回避态度.中药十八反在基层医院的应用是客观存在的,并非绝对的禁总.

  14. Functional divergence caused by ancient positive selection of a Drosophila hybrid incompatibility locus.

    Directory of Open Access Journals (Sweden)

    Daniel A Barbash

    2004-06-01

    Full Text Available Interspecific hybrid lethality and sterility are a consequence of divergent evolution between species and serve to maintain the discrete identities of species. The evolution of hybrid incompatibilities has been described in widely accepted models by Dobzhansky and Muller where lineage-specific functional divergence is the essential characteristic of hybrid incompatibility genes. Experimentally tractable models are required to identify and test candidate hybrid incompatibility genes. Several Drosophila melanogaster genes involved in hybrid incompatibility have been identified but none has yet been shown to have functionally diverged in accordance with the Dobzhansky-Muller model. By introducing transgenic copies of the X-linked Hybrid male rescue (Hmr gene into D. melanogaster from its sibling species D. simulans and D. mauritiana, we demonstrate that Hmr has functionally diverged to cause F1 hybrid incompatibility between these species. Consistent with the Dobzhansky-Muller model, we find that Hmr has diverged extensively in the D. melanogaster lineage, but we also find extensive divergence in the sibling-species lineage. Together, these findings implicate over 13% of the amino acids encoded by Hmr as candidates for causing hybrid incompatibility. The exceptional level of divergence at Hmr cannot be explained by neutral processes because we use phylogenetic methods and population genetic analyses to show that the elevated amino-acid divergence in both lineages is due to positive selection in the distant past-at least one million generations ago. Our findings suggest that multiple substitutions driven by natural selection may be a general phenomenon required to generate hybrid incompatibility alleles.

  15. Whole genome association mapping by incompatibilities and local perfect phylogenies

    Directory of Open Access Journals (Sweden)

    Besenbacher Søren

    2006-10-01

    Full Text Available Abstract Background With current technology, vast amounts of data can be cheaply and efficiently produced in association studies, and to prevent data analysis to become the bottleneck of studies, fast and efficient analysis methods that scale to such data set sizes must be developed. Results We present a fast method for accurate localisation of disease causing variants in high density case-control association mapping experiments with large numbers of cases and controls. The method searches for significant clustering of case chromosomes in the "perfect" phylogenetic tree defined by the largest region around each marker that is compatible with a single phylogenetic tree. This perfect phylogenetic tree is treated as a decision tree for determining disease status, and scored by its accuracy as a decision tree. The rationale for this is that the perfect phylogeny near a disease affecting mutation should provide more information about the affected/unaffected classification than random trees. If regions of compatibility contain few markers, due to e.g. large marker spacing, the algorithm can allow the inclusion of incompatibility markers in order to enlarge the regions prior to estimating their phylogeny. Haplotype data and phased genotype data can be analysed. The power and efficiency of the method is investigated on 1 simulated genotype data under different models of disease determination 2 artificial data sets created from the HapMap ressource, and 3 data sets used for testing of other methods in order to compare with these. Our method has the same accuracy as single marker association (SMA in the simplest case of a single disease causing mutation and a constant recombination rate. However, when it comes to more complex scenarios of mutation heterogeneity and more complex haplotype structure such as found in the HapMap data our method outperforms SMA as well as other fast, data mining approaches such as HapMiner and Haplotype Pattern Mining (HPM

  16. Elevated Fasting Blood Glucose Is Predictive of Poor Outcome in Non-Diabetic Stroke Patients: A Sub-Group Analysis of SMART

    Science.gov (United States)

    Zhou, Lixin; Peng, Bin; Zhu, Yicheng; Cui, Liying

    2016-01-01

    Background Although increasing evidence suggests that hyperglycemia following acute stroke adversely affects clinical outcome, whether the association between glycaemia and functional outcome varies between stroke patients with\\without pre-diagnosed diabetes remains controversial. We aimed to investigate the relationship between the fasting blood glucose (FBG) and the 6-month functional outcome in a subgroup of SMART cohort and further to assess whether this association varied based on the status of pre-diagnosed diabetes. Methods Data of 2862 patients with acute ischemic stroke (629 with pre-diagnosed diabetics) enrolled from SMART cohort were analyzed. Functional outcome at 6-month post-stroke was measured by modified Rankin Scale (mRS) and categorized as favorable (mRS:0–2) or poor (mRS:3–5). Binary logistic regression model, adjusting for age, gender, educational level, history of hypertension and stroke, baseline NIHSS and treatment group, was used in the whole cohort to evaluate the association between admission FBG and functional outcome. Stratified logistic regression analyses were further performed based on the presence/absence of pre-diabetes history. Results In the whole cohort, multivariable logistical regression showed that poor functional outcome was associated with elevated FBG (OR1.21 (95%CI 1.07–1.37), p = 0.002), older age (OR1.64 (95% CI1.38–1.94), p<0.001), higher NIHSS (OR2.90 (95%CI 2.52–3.33), p<0.001) and hypertension (OR1.42 (95%CI 1.13–1.98), p = 0.04). Stratified logistical regression analysis showed that the association between FBG and functional outcome remained significant only in patients without pre-diagnosed diabetes (OR1.26 (95%CI 1.03–1.55), p = 0.023), but not in those with premorbid diagnosis of diabetes (p = 0.885). Conclusion The present results demonstrate a significant association between elevated FBG after stroke and poor functional outcome in patients without pre-diagnosed diabetes, but not in diabetics

  17. The impact of storage time of transfused blood on postoperative infectious complications in rectal cancer surgery. Danish RANX05 Colorectal Cancer Study Group

    DEFF Research Database (Denmark)

    Mynster, T; Nielsen, Hans Jørgen

    2000-01-01

    BACKGROUND: We have studied the impact of storage time of transfused allogeneic blood together with other known risk factors on postoperative infectious complications after operation for rectal cancer. METHODS: Intra-abdominal abscess, anastomotic leakage, septicaemia, wound infection...... and storage time of saline-adenine-glucose-mannitol (SAGM) blood, administered to each patient, were recorded retrospectively. RESULTS: The overall infection rate was 24% in 78 non-transfused and 40% in 225 transfused patients (P = 0.011). The proportion of SAGM blood stored for > or = 21 days administered...... days (odds ratio, 2.5 versus no transfusion) to be independent variables predicting infectious complications. CONCLUSION: Blood storage time may, along with other risk factors, play a significant role in blood transfusion-associated development of postoperative infectious complications....

  18. Extreme incompatibility of helium during mantle melting: Evidence from undegassed mid-ocean ridge basalts

    Science.gov (United States)

    Graham, David W.; Michael, Peter J.; Shea, Thomas

    2016-11-01

    We report total helium concentrations (vesicles + glass) for a suite of thirteen ultradepleted mid-ocean ridge basalts (UD-MORBs) that were previously studied for volatile contents (CO2, H2O) plus major and trace elements. The selected basalts are undersaturated in CO2 + H2O at their depths of eruption and represent rare cases of undegassed MORBs. Sample localities from the Atlantic (2), Indian (1) and Pacific (7) Oceans collectively show excellent linear correlations (r2 = 0.75- 0.92) between the concentrations of helium and the highly incompatible elements C, K, Rb, Ba, Nb, Th and U. Three basalts from Gakkel Ridge in the Arctic were also studied but show anomalous behavior marked by excess lithophile trace element abundances. In the Atlantic-Pacific-Indian suite, incompatible element concentrations vary by factors of 3-4.3, while helium concentration varies by a factor of 13. The strong correlations between the concentrations of helium and incompatible elements are explained by helium behavior as the most incompatible element during mantle melting. Partial melting of an ultradepleted mantle source, formed as a residue of earlier melt extraction, accounts for the observed concentrations. The earlier melting event involved removal of a small degree melt (∼1%) at low but non-zero porosity (0.01-0.5%), leading to a small amount of melt retention that strongly leveraged the incompatible element budget of the ultradepleted mantle source. Equilibrium melting models that produce the range of trace element and helium concentrations from this source require a bulk solid/melt distribution coefficient for helium that is lower than that for other incompatible elements by about a factor of ten. Alternatively, the bulk solid/melt distribution coefficient for helium could be similar to or even larger than that for other incompatible elements, but the much larger diffusivity of helium in peridotite leads to its more effective incompatibility and efficient extraction from a

  19. Incompatible pollen tubes in the plum style and their impact on fertilization success

    Directory of Open Access Journals (Sweden)

    Đorđević Milena

    2014-01-01

    Full Text Available The pistils of plum (Prunus domestica L. cv. ‘Čačanska Lepotica’ were self- and cross-pollinated in order to investigate occurrence of incompatible pollen tubes in the style, and their impact on fertilization success. The investigation also included open pollination variant. The highest percentage of these stopped the growth in the upper third of the style. Under cross-pollination variant, 15.4% and 12.1% of pollen tubes observed in the upper part of the style in the first and the second years of study, respectively, were found incompatible. In view of the above parameters, in the self- pollination variant, 15.0% and 17.0% of pollen tubes were found incompatible by years. As for the open pollination, percentages of incompatible pollen tubes in the upper part of the style by years were 14.0% and 14.4%, respectively. The occurrence of incompatible pollen tubes did not influence the fertilization success in these pollination variants. [Projekat Ministarstva nauke Republike Srbije, br. TR31064-Development and preservation of genetic potential of temperate zone fruits

  20. Strength through unity: spatial affinity between morphs improves fitness in incompatible heterostylous Melochia (Malvaceae) species.

    Science.gov (United States)

    Faife-Cabrera, Michel; Navarro, Luis; Ferrero, Victoria

    2015-01-01

    In heterostylous plants, both stylar polymorphism and incompatibility system favor legitimate pollination among individuals. Weak or partial expression of incompatibility may ensure progeny when mates or pollinators are scarce in unstable habitats, but under these conditions plants with heteromorphic incompatibility would be in disadvantage. In this work we determine how the spatial distribution of morphs and the effect of proximity to the nearest potential mates affect plants' reproductive output in four Melochia species. The general prediction of decreasing reproductive success with an increasing isolation of floral morphs in plants with heteromorphic incompatibility was corroborated only in one species (i.e. M. tomentosa). Meanwhile, the other species exhibit a spatial affinity between morphs (i.e. the number of individuals with the nearest neighbor of the opposite morph exceeds expectations upon a random distribution). For M. savannarum and M. villosa we could not detect any effect of proximity to potential mates on the seed-ovule ratio. This may be due to: (1) existence of pollinators with long flying distances, like butterflies, in the populations and/or, (2) the possible occurrence of resource limitation. Spatial affinity between morphs in populations of heterostylous plants with heteromorphic incompatibility system increases reproductive success and may facilitate colonization of ephemeral habitats.

  1. An Empirical Comparison of Joint and Stratified Frameworks for Studying G × E Interactions: Systolic Blood Pressure and Smoking in the CHARGE Gene-Lifestyle Interactions Working Group.

    Science.gov (United States)

    Sung, Yun Ju; Winkler, Thomas W; Manning, Alisa K; Aschard, Hugues; Gudnason, Vilmundur; Harris, Tamara B; Smith, Albert V; Boerwinkle, Eric; Brown, Michael R; Morrison, Alanna C; Fornage, Myriam; Lin, Li-An; Richard, Melissa; Bartz, Traci M; Psaty, Bruce M; Hayward, Caroline; Polasek, Ozren; Marten, Jonathan; Rudan, Igor; Feitosa, Mary F; Kraja, Aldi T; Province, Michael A; Deng, Xuan; Fisher, Virginia A; Zhou, Yanhua; Bielak, Lawrence F; Smith, Jennifer; Huffman, Jennifer E; Padmanabhan, Sandosh; Smith, Blair H; Ding, Jingzhong; Liu, Yongmei; Lohman, Kurt; Bouchard, Claude; Rankinen, Tuomo; Rice, Treva K; Arnett, Donna; Schwander, Karen; Guo, Xiuqing; Palmas, Walter; Rotter, Jerome I; Alfred, Tamuno; Bottinger, Erwin P; Loos, Ruth J F; Amin, Najaf; Franco, Oscar H; van Duijn, Cornelia M; Vojinovic, Dina; Chasman, Daniel I; Ridker, Paul M; Rose, Lynda M; Kardia, Sharon; Zhu, Xiaofeng; Rice, Kenneth; Borecki, Ingrid B; Rao, Dabeeru C; Gauderman, W James; Cupples, L Adrienne

    2016-07-01

    Studying gene-environment (G × E) interactions is important, as they extend our knowledge of the genetic architecture of complex traits and may help to identify novel variants not detected via analysis of main effects alone. The main statistical framework for studying G × E interactions uses a single regression model that includes both the genetic main and G × E interaction effects (the "joint" framework). The alternative "stratified" framework combines results from genetic main-effect analyses carried out separately within the exposed and unexposed groups. Although there have been several investigations using theory and simulation, an empirical comparison of the two frameworks is lacking. Here, we compare the two frameworks using results from genome-wide association studies of systolic blood pressure for 3.2 million low frequency and 6.5 million common variants across 20 cohorts of European ancestry, comprising 79,731 individuals. Our cohorts have sample sizes ranging from 456 to 22,983 and include both family-based and population-based samples. In cohort-specific analyses, the two frameworks provided similar inference for population-based cohorts. The agreement was reduced for family-based cohorts. In meta-analyses, agreement between the two frameworks was less than that observed in cohort-specific analyses, despite the increased sample size. In meta-analyses, agreement depended on (1) the minor allele frequency, (2) inclusion of family-based cohorts in meta-analysis, and (3) filtering scheme. The stratified framework appears to approximate the joint framework well only for common variants in population-based cohorts. We conclude that the joint framework is the preferred approach and should be used to control false positives when dealing with low-frequency variants and/or family-based cohorts. PMID:27230302

  2. Crystal Structures of GII.10 and GII.12 Norovirus Protruding Domains in Complex with Histo-Blood Group Antigens Reveal Details for a Potential Site of Vulnerability

    Energy Technology Data Exchange (ETDEWEB)

    Hansman, Grant S.; Biertümpfel, Christian; Georgiev, Ivelin; McLellan, Jason S.; Chen, Lei; Zhou, Tongqing; Katayama, Kazuhiko; Kwong, Peter D. (NIH); (NIID-Japan)

    2011-10-10

    Noroviruses are the dominant cause of outbreaks of gastroenteritis worldwide, and interactions with human histo-blood group antigens (HBGAs) are thought to play a critical role in their entry mechanism. Structures of noroviruses from genogroups GI and GII in complex with HBGAs, however, reveal different modes of interaction. To gain insight into norovirus recognition of HBGAs, we determined crystal structures of norovirus protruding domains from two rarely detected GII genotypes, GII.10 and GII.12, alone and in complex with a panel of HBGAs, and analyzed structure-function implications related to conservation of the HBGA binding pocket. The GII.10- and GII.12-apo structures as well as the previously solved GII.4-apo structure resembled each other more closely than the GI.1-derived structure, and all three GII structures showed similar modes of HBGA recognition. The primary GII norovirus-HBGA interaction involved six hydrogen bonds between a terminal {alpha}fucose1-2 of the HBGAs and a dimeric capsid interface, which was composed of elements from two protruding subdomains. Norovirus interactions with other saccharide units of the HBGAs were variable and involved fewer hydrogen bonds. Sequence analysis revealed a site of GII norovirus sequence conservation to reside under the critical {alpha}fucose1-2 and to be one of the few patches of conserved residues on the outer virion-capsid surface. The site was smaller than that involved in full HBGA recognition, a consequence of variable recognition of peripheral saccharides. Despite this evasion tactic, the HBGA site of viral vulnerability may provide a viable target for small molecule- and antibody-mediated neutralization of GII norovirus.

  3. Structural Analysis of Histo-Blood Group Antigen Binding Specificity in a Norovirus GII.4 Epidemic Variant: Implications for Epochal Evolution

    Energy Technology Data Exchange (ETDEWEB)

    Shanker, Sreejesh; Choi, Jae-Mun; Sankaran, Banumathi; Atmar, Robert L.; Estes, Mary K.; Prasad, B.V. Venkataram (Baylor); (LBNL)

    2012-03-23

    Susceptibility to norovirus (NoV), a major pathogen of epidemic gastroenteritis, is associated with histo-blood group antigens (HBGAs), which are also cell attachment factors for this virus. GII.4 NoV strains are predominantly associated with worldwide NoV epidemics with a periodic emergence of new variants. The sequence variations in the surface-exposed P domain of the capsid protein resulting in differential HBGA binding patterns and antigenicity are suggested to drive GII.4 epochal evolution. To understand how temporal sequence variations affect the P domain structure and contribute to epochal evolution, we determined the P domain structure of a 2004 variant with ABH and secretor Lewis HBGAs and compared it with the previously determined structure of a 1996 variant. We show that temporal sequence variations do not affect the binding of monofucosyl ABH HBGAs but that they can modulate the binding strength of difucosyl Lewis HBGAs and thus could contribute to epochal evolution by the potentiated targeting of new variants to Lewis-positive, secretor-positive individuals. The temporal variations also result in significant differences in the electrostatic landscapes, likely reflecting antigenic variations. The proximity of some of these changes to the HBGA binding sites suggests the possibility of a coordinated interplay between antigenicity and HBGA binding in epochal evolution. From the observation that the regions involved in the formation of the HBGA binding sites can be conformationally flexible, we suggest a plausible mechanism for how norovirus disassociates from salivary mucin-linked HBGA before reassociating with HBGAs linked to intestinal epithelial cells during its passage through the gastrointestinal tract.

  4. Lewis histo-blood group α1,3/α1,4 fucose residues may both mediate binding to GII.4 noroviruses.

    Science.gov (United States)

    Nasir, Waqas; Frank, Martin; Koppisetty, Chaitanya A K; Larson, Göran; Nyholm, Per-Georg

    2012-09-01

    Human noroviruses cause recurrent epidemics of gastroenteritis known to be dominated by the clinically important GII.4 genotype which recognizes human Secretor gene-dependent ABH histo-blood group antigens (HBGAs) as attachment factors. There is increasing evidence that GII.4 noroviruses have undergone evolutionary changes to recognize Lewis antigens and non-Secretor saliva. In this study, we have investigated the possibilities of the Lewis α1,3/α1,4 fucoses as mediators of binding of GII.4 noroviruses to Lewis antigens. The study was carried out using molecular dynamics simulations of Lewis type-1 and type-2 chain HBGAs in complex with VA387 P domain dimers in explicit water. Based on the computer simulations, we suggest the possibility of two receptor binding modes for Lewis HBGAs: the "Secretor pose" with the Secretor Fucα1,2 in the binding site and the "Lewis pose" with the Lewis Fucα1,3/α1,4 residues in the binding site. This was further supported by an extensive GlyVicinity analysis of the Protein Data Bank with respect to the occurrence of the Lewis and Secretor poses in complexes of Lewis antigens with lectins and antibodies as well as GII norovirus strains. The Lewis pose can also explain the interactions of GII.4 norovirus strains with Le(x) and SLe(x) structures. Moreover, the present model suggests binding of complex branched polysaccharides, with the Lewis antigens at the nonreducing end, to P domain dimers of GII.4 strains. Our results are relevant for understanding the evolution of norovirus binding specificities and for in silico design of future antiviral therapeutics.

  5. Crystallography of a Lewis-binding norovirus, elucidation of strain-specificity to the polymorphic human histo-blood group antigens.

    Directory of Open Access Journals (Sweden)

    Yutao Chen

    2011-07-01

    Full Text Available Noroviruses, an important cause of acute gastroenteritis in humans, recognize the histo-blood group antigens (HBGAs as host susceptible factors in a strain-specific manner. The crystal structures of the HBGA-binding interfaces of two A/B/H-binding noroviruses, the prototype Norwalk virus (GI.1 and a predominant GII.4 strain (VA387, have been elucidated. In this study we determined the crystal structures of the P domain protein of the first Lewis-binding norovirus (VA207, GII.9 that has a distinct binding property from those of Norwalk virus and VA387. Co-crystallization of the VA207 P dimer with Le(y or sialyl Le(x tetrasaccharides showed that VA207 interacts with these antigens through a common site found on the VA387 P protein which is highly conserved among most GII noroviruses. However, the HBGA-binding site of VA207 targeted at the Lewis antigens through the α-1, 3 fucose (the Lewis epitope as major and the β-N-acetyl glucosamine of the precursor as minor interacting sites. This completely differs from the binding mode of VA387 and Norwalk virus that target at the secretor epitopes. Binding pocket of VA207 is formed by seven amino acids, of which five residues build up the core structure that is essential for the basic binding function, while the other two are involved in strain-specificity. Our results elucidate for the first time the genetic and structural basis of strain-specificity by a direct comparison of two genetically related noroviruses in their interaction with different HBGAs. The results provide insight into the complex interaction between the diverse noroviruses and the polymorphic HBGAs and highlight the role of human HBGA as a critical factor in norovirus evolution.

  6. 正常高值血压青年人群生活方式干预效果评价%Results of Life Style Intervention in Young Group of High-normal Blood Pressure

    Institute of Scientific and Technical Information of China (English)

    杨久亮

    2014-01-01

    目的:评价正常高值血压青年人群生活方式的干预效果。方法:纳入2010年10月-2012年10月正常高值血压青年人群120例。将其随机分为干预组与对照组,各60例。对照组不进行任何干预,干预组进行2年的生活方式干预,对实施前后血压、血脂、体重指数、高血压认知度进行对比。结果:通过2年生活方式干预后,干预组血压、体重下降明显,高血压认知率明显提高,与对照组比较,差异有统计学意义(P<0.05)。结论:对正常高值血压人群实施生活方式干预,可有效降低血压及高血压危险因素。%Objective:To review the results of life style intervention in young people group of high-normal blood pressure. Method:The young people with high-normal blood pressure were selected from October 2010 to October 2012,and then randomly divided into intervention group(n=60) and control group(n=60). The control group without any intervention,the intervention group was given life style intervention.The changes of blood pressure,blood fat,body mass index (BMI) and disease cognition were compared before and after the intervention.Result:In intervention group,blood pressure and BMI decreased significantly and disease cognition increased significantly through life style intervention for 2 years.There was significant difference compared with control group(P<0.05).Conclusion:The life style intervention in young people group of high-normal blood pressure can effectively reduce blood pressure and risk factors of hypertension.

  7. Blood transfusions

    Science.gov (United States)

    ... called homologous blood donation. Many communities have a blood bank at which any healthy person can donate blood. ... need to arrange with your hospital or local blood bank before your surgery to have directed donor blood. ...

  8. Overcoming interspecific incompatibility in the cross Brassica campestris ssp. japonica x Brassica oleracea var. botrytis using irradiated mentor pollen page

    International Nuclear Information System (INIS)

    The cross B. campestris ssp. japonica x B. oleracea var. botrytis fails due to incompatibility barrier at the stigma. To realize this cross, irradiated compatible pollen (mentor pollen) was used before the incompatible pollination. The presence of mentor pollen stimulated the incompatible pollen to germinate and effect fertilization and seed set. One hybrid was thus obtained. Most of the seeds were inviable. Of the 5 plants raised one was a hybrid and 4 resembled the female parent. 1 tab., 7 refs

  9. Quantitatively determined self-incompatibility : 2. Outcrossing inBorago officinaux.

    Science.gov (United States)

    Leach, C R; Mayo, O; Bürger, R

    1990-05-01

    It has been claimed that Borage (Borago officInalis L.) has a multifactorial self-incompatibility system. Such systems may have a high level of ineffective pollination, and we show that this is the case in borage. The ranking of seed set from highest to lowest is as follows: bee-pollination; natural pollination in the absence of bees; artificial cross-pollination between unrelated plants; artificial cross-pollination between related plants; artificial self-pollination. In diallel crosses, significant parental effects were detected but no consistent patterns of seed set, which suggest a simple self-incompatibility system, were detected. The level of outcrossing with natural pollination was very variable but greater than 50%. Thus, there appears to be no straightforward self-incompatibility system in borage. PMID:24226365

  10. A comparative study of process mediator components that support behavioral incompatibility

    CERN Document Server

    Munusamy, Kanmani; Ibrahim, Suhaimi; Baba, Mohd Sapiyan

    2011-01-01

    Most businesses these days use the web services technology as a medium to allow interaction between a service provider and a service requestor. However, both the service provider and the requestor would be unable to achieve their business goals when there are miscommunications between their processes. This research focuses on the process incompatibility between the web services and the way to automatically resolve them by using a process mediator. This paper presents an overview of the behavioral incompatibility between web services and the overview of process mediation in order to resolve the complications faced due to the incompatibility. Several state-of the-art approaches have been selected and analyzed to understand the existing process mediation components. This paper aims to provide a valuable gap analysis that identifies the important research areas in process mediation that have yet to be fully explored.

  11. Allele distributions at hybrid incompatibility loci facilitate the potential for gene flow between cultivated and weedy rice in the US.

    Directory of Open Access Journals (Sweden)

    Stephanie M Craig

    Full Text Available The accumulation of independent mutations over time in two populations often leads to reproductive isolation. Reproductive isolation between diverging populations may be reinforced by barriers that occur either pre- or postzygotically. Hybrid sterility is the most common form of postzygotic isolation in plants. Four postzygotic sterility loci, comprising three hybrid sterility systems (Sa, s5, DPL, have been recently identified in Oryza sativa. These loci explain, in part, the limited hybridization that occurs between the domesticated cultivated rice varieties, O. sativa spp. japonica and O. sativa spp. indica. In the United States, cultivated fields of japonica rice are often invaded by conspecific weeds that have been shown to be of indica origin. Crop-weed hybrids have been identified in crop fields, but at low frequencies. Here we examined the possible role of these hybrid incompatibility loci in the interaction between cultivated and weedy rice. We identified a novel allele at Sa that seemingly prevents loss of fertility in hybrids. Additionally, we found wide-compatibility type alleles at strikingly high frequencies at the Sa and s5 loci in weed groups, and a general lack of incompatible alleles between crops and weeds at the DPL loci. Our results suggest that weedy individuals, particularly those of the SH and BRH groups, should be able to freely hybridize with the local japonica crop, and that prezygotic factors, such as differences in flowering time, have been more important in limiting weed-crop gene flow in the past. As the selective landscape for weedy rice changes due to increased use of herbicide resistant strains of cultivated rice, the genetic barriers that hinder indica-japonica hybridization cannot be counted on to limit the flow of favorable crop genes into weeds.

  12. Distinguishing early-acting inbreeding depression from late-acting ovar-ian self-incompatibility

    OpenAIRE

    Yiqi Hao; Xinfeng Zhao

    2011-01-01

    Reduced seed yields after self-pollination are generally thought to be induced by early-acting in-breeding depression and self-incompatibility. Early-acting inbreeding depression occurs strictly post-zygoti- cally, and leads to the abortion of progeny that are homozygous for deleterious recessive alleles at an early stage of seed maturation. Late-acting ovarian self-incompatibility, on the other hand, may be either pre- or post-zygotic, and usually only one locus is responsible for the reject...

  13. Difficulty of ABO blood group specimen phenotype and gene detection%疑难 ABO 血型标本的表型与相应等位基因的检测

    Institute of Scientific and Technical Information of China (English)

    王莹

    2016-01-01

    Objective To study the analysis of difficult specimens of ABO blood group and its allele detection ,for clinical encounter difficulty provide reliable blood specimens of ABO blood group identification method .Methods Se‐lected during August 2013 to August 2014 working in the hospital each department makes clinical patient blood samples from 9 cases ,first of all ,according to the conventional blood detection techniques for ABO serological test ,determine ABO positive and negative stereotypes ,again using guanidine hydrochloride/proteinase K cracking after extraction of ETDA anticoagulant treatment of peripheral vein for DNA extraction ,then using the method of polymerase chain reac‐tion sequence specific primers (PCR SSP) ABO blood group 9 cases of blood sample genotyping detection .Results 9 cases of clinical serological phenotype for patients :AxB in 2 cases ,AB ,and 1 case ,and A and B ,and 1 case ,Ax in 2 ca‐ses ,B ,and 3 cases ,9 cases of patients with genotype ,respectively is :A/B 4 cases ,2 cases of A/O ,B/O 3 cases ,meas‐ured by PCR‐SSP genotype is consistent with the results of serological phenotype .Conclusion Using the method of polymerase chain reaction sequence specific primers (PCR SSP) ABO blood group identification results of serological method for positive and negative don't finalize the design difficulty of blood specimens tested genotypes ,to accurately determine the patient's blood group antigen ,for clinical patients with complicated ABO blood group provide guarantee for safe and effective blood scheme .%目的:探讨分析疑难ABO血型标本与其等位基因的检测,为临床遇到的疑难ABO血型标本提供可靠的血型鉴定方法。方法选取2013年8月到2014年8月期间在笔者就职医院各科室送检的临床病人血液样本9例,首先按照常规血型检测技术进行ABO血型血清学试验,确定ABO正反定型,再采用盐酸胍/蛋白酶K裂解提取法对ETDA抗凝处理后的静脉外周

  14. Secondary evolution of a self-incompatibility locus in the Brassicaceae genus Leavenworthia.

    Directory of Open Access Journals (Sweden)

    Sier-Ching Chantha

    Full Text Available Self-incompatibility (SI is the flowering plant reproductive system in which self pollen tube growth is inhibited, thereby preventing self-fertilization. SI has evolved independently in several different flowering plant lineages. In all Brassicaceae species in which the molecular basis of SI has been investigated in detail, the product of the S-locus receptor kinase (SRK gene functions as receptor in the initial step of the self pollen-rejection pathway, while that of the S-locus cysteine-rich (SCR gene functions as ligand. Here we examine the hypothesis that the S locus in the Brassicaceae genus Leavenworthia is paralogous with the S locus previously characterized in other members of the family. We also test the hypothesis that self-compatibility in this group is based on disruption of the pollen ligand-producing gene. Sequence analysis of the S-locus genes in Leavenworthia, phylogeny of S alleles, gene expression patterns, and comparative genomics analyses provide support for both hypotheses. Of special interest are two genes located in a non-S locus genomic region of Arabidopsis lyrata that exhibit domain structures, sequences, and phylogenetic histories similar to those of the S-locus genes in Leavenworthia, and that also share synteny with these genes. These A. lyrata genes resemble those comprising the A. lyrata S locus, but they do not function in self-recognition. Moreover, they appear to belong to a lineage that diverged from the ancestral Brassicaceae S-locus genes before allelic diversification at the S locus. We hypothesize that there has been neo-functionalization of these S-locus-like genes in the Leavenworthia lineage, resulting in evolution of a separate ligand-receptor system of SI. Our results also provide support for theoretical models that predict that the least constrained pathway to the evolution of self-compatibility is one involving loss of pollen gene function.

  15. Non-immune binding of human IgG to M-related proteins confers resistance to phagocytosis of group A streptococci in blood.

    Directory of Open Access Journals (Sweden)

    Harry S Courtney

    Full Text Available The non-immune binding of immunoglobulins by bacteria is thought to contribute to the pathogenesis of infections. M-related proteins (Mrp are group A streptococcal (GAS receptors for immunoglobulins, but it is not known if this binding has any impact on virulence. To further investigate the binding of immunoglobulins to Mrp, we engineered mutants of an M type 4 strain of GAS by inactivating the genes for mrp, emm, enn, sof, and sfbX and tested these mutants in IgG-binding assays. Inactivation of mrp dramatically decreased the binding of human IgG, whereas inactivation of emm, enn, sof, and sfbx had only minor effects, indicating that Mrp is a major IgG-binding protein. Binding of human immunoglobulins to a purified, recombinant form of Mrp indicated that it selectively binds to the Fc domain of human IgG, but not IgA or IgM and that it preferentially bound subclasses IgG₁>IgG₄>IgG₂>IgG₃. Recombinant proteins encompassing different regions of Mrp were engineered and used to map its IgG-binding domain to its A-repeat region and a recombinant protein with 3 A-repeats was a better inhibitor of IgG binding than one with a single A-repeat. A GAS mutant expressing Mrp with an in-frame deletion of DNA encoding the A-repeats had a dramatically reduced ability to bind human IgG and to grow in human blood. Mrp exhibited host specificity in binding IgG; human IgG was the best inhibitor of the binding of IgG followed by pig, horse, monkey, and rabbit IgG. IgG from goat, mouse, rat, cow, donkey, chicken, and guinea pig were poor inhibitors of binding. These findings indicate that Mrp preferentially binds human IgG and that this binding contributes to the ability of GAS to resist phagocytosis and may be a factor in the restriction of GAS infections to the human host.

  16. New incompatibilities uncovered using the Promega DNA IQ™ chemistry.

    Science.gov (United States)

    Laurin, Nancy; Célestin, Florence; Clark, Meagan; Wilkinson, Della; Yamashita, Brian; Frégeau, Chantal

    2015-12-01

    Over the years, the Promega DNA IQ™ System was proven an effective technology for the production of clean DNA from a wide variety of casework specimens. The capture of DNA using the DNA IQ™ paramagnetic beads, however, was shown to be affected by a few specific chemicals that could be present on exhibits submitted to the laboratory. In this study, various blood and latent fingerprint enhancement reagents/methods, marker pens and adhesive tapes, applied at the crime scene or in the forensic laboratory on casework exhibits or used to collect biological material, were tested for their compatibility with the DNA IQ™ technology. Although no impact on DNA recovery was observed for most reagents, the MAGNA™ Jet Black fingerprint powder and three 3M Scotch(®) adhesive tapes were shown to severely or completely inhibit DNA binding onto the DNA IQ™ beads. The effect of MAGNA™ Jet Black on DNA recovery could be counteracted by separating the magnetic powder from the lysates by centrifugation or filtration, prior to DNA extraction. High quality STR profiles were obtained from samples subjected to MAGNA™ Jet Black suggesting it does not impact DNA integrity.

  17. New incompatibilities uncovered using the Promega DNA IQ™ chemistry.

    Science.gov (United States)

    Laurin, Nancy; Célestin, Florence; Clark, Meagan; Wilkinson, Della; Yamashita, Brian; Frégeau, Chantal

    2015-12-01

    Over the years, the Promega DNA IQ™ System was proven an effective technology for the production of clean DNA from a wide variety of casework specimens. The capture of DNA using the DNA IQ™ paramagnetic beads, however, was shown to be affected by a few specific chemicals that could be present on exhibits submitted to the laboratory. In this study, various blood and latent fingerprint enhancement reagents/methods, marker pens and adhesive tapes, applied at the crime scene or in the forensic laboratory on casework exhibits or used to collect biological material, were tested for their compatibility with the DNA IQ™ technology. Although no impact on DNA recovery was observed for most reagents, the MAGNA™ Jet Black fingerprint powder and three 3M Scotch(®) adhesive tapes were shown to severely or completely inhibit DNA binding onto the DNA IQ™ beads. The effect of MAGNA™ Jet Black on DNA recovery could be counteracted by separating the magnetic powder from the lysates by centrifugation or filtration, prior to DNA extraction. High quality STR profiles were obtained from samples subjected to MAGNA™ Jet Black suggesting it does not impact DNA integrity. PMID:26295931

  18. The impact of storage time of transfused blood on postoperative infectious complications in rectal cancer surgery. Danish RANX05 Colorectal Cancer Study Group

    DEFF Research Database (Denmark)

    Mynster, T; Nielsen, Hans Jørgen

    2000-01-01

    BACKGROUND: We have studied the impact of storage time of transfused allogeneic blood together with other known risk factors on postoperative infectious complications after operation for rectal cancer. METHODS: Intra-abdominal abscess, anastomotic leakage, septicaemia, wound infection, and pneumo......BACKGROUND: We have studied the impact of storage time of transfused allogeneic blood together with other known risk factors on postoperative infectious complications after operation for rectal cancer. METHODS: Intra-abdominal abscess, anastomotic leakage, septicaemia, wound infection...... and storage time of saline-adenine-glucose-mannitol (SAGM) blood, administered to each patient, were recorded retrospectively. RESULTS: The overall infection rate was 24% in 78 non-transfused and 40% in 225 transfused patients (P = 0.011). The proportion of SAGM blood stored for > or = 21 days administered...... to each transfused patient was a median of 60% in patients developing postoperative infections versus 25% (P = 0.037) in patients without infections. A multivariate analysis of significant risk variables showed weight > 75 kg (odds ratio, 2.0 versus blood stored > or = 21...

  19. Mantle in the Manihiki Plateau source with ultra-depleted incompatible element abundances but FOZO-like isotopic signature

    Science.gov (United States)

    Golowin, R.; Hoernle, K.; Portnyagin, M.; Hauff, F.; Gurenko, A.; Garbe-Schoenberg, C. D.; Werner, R.

    2014-12-01

    The ~120Ma Manihiki Plateau basement consists of high-Ti tholeiitic basalts with EM-I type isotopic signatures, similar to the Singgalo basalts at Ontong Java, and low-Ti tholeiitic basalts with FOZO (Kwaimbaita/Kroenke) to HIMU-type isotopic compositions, similar to late stage volcanism on Hikurangi and Manihiki Plateaus (Hoernle et al. 2010; Timm et al. 2011). The low-Ti basalts have affinities to boninites and have been interpreted to be derived from residual mantle wedge mantle (Ingle et al. 2007). New major, volatile and trace element and radiogenic isotope data have been generated from fresh low-Ti glass samples recovered during R/V Sonne cruises SO193 and SO225. The low-Ti samples have distinctly lower Ti/V ratios compared to lavas from Ontong Java Plateau (Kwaimbaita-Kroenke and Singgalo), but similar to boninitic rocks. Glasses and melt inclusions in olivine have low volatile contents (0.12-0.25 wt% H2O). Olivine chemistry points to derivation from peridotite source. Therefore we interpret the low-Ti lavas to have formed through melting of dry and depleted peridotite at high temperatures, consistent with Timm et al (2011). The low-Ti group is characterized by U-shaped trace element patterns. The glass samples form linear mixing arrays on radiogenic isotope diagrams, pointing to the involvement of two components: 1) a component ultra-depleted in highly incompatible elements (UDC) but with intermediate Pb, Sr and Nd isotopic compositions, being similar to Kwaimbaita/Kroenke lavas from Ontong Java, and 2) an enriched component with HIMU-type incompatible element and isotopic characteristics, similar to late-stage volcanism on Manihiki, Hikurangi and Ontong Java (e.g. Hoernle et al. 2010). The ultra-depleted, FOZO-like mantle component could represent second stage melting of FOZO type mantle or re-melting of young recycled oceanic lithosphere within the plume head. Enrichment with HIMU type melts is required to explain the enrichment in the most incompatible

  20. Blood Donation Management System

    OpenAIRE

    K M Akkas Ali; Israt Jahan; Md. Ariful Islam; Md. Shafa-at Parvez

    2015-01-01

    This paper is focused on Blood Donation Management System which is a web application with supporting mobile application aimed to serve as a communication tool between patients (who need blood) and blood donor. To become members of the system, donors need to create their profiles by providing fundamental information like name, blood group, email address, password, and exact location from “Google Map”. In order to find out the exact location of a donor, Google Map is integrated with this app...