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Sample records for blood donor educational

  1. Health education about AIDS among seropositive blood donors.

    Science.gov (United States)

    Cleary, P D; Rogers, T F; Singer, E; Avorn, J; van Devanter, N; Perry, S; Pindyck, J

    1986-01-01

    The New York Blood Center is developing a health education and psychosocial support program for blood donors who are notified that they are HIV antibody positive. The goals of that program are: to provide accurate and intelligible information about the test results to notified donors; to encourage behavior that will reduce the likelihood of spreading the virus; to encourage notified donors to behave in ways that will reduce the probability that they will develop AIDS; and to provide support and facilitate functional coping responses. This article reviews the theoretical and empirical work which informs the intervention program, and it describes how the program is being implemented. PMID:3023260

  2. Modelling antecedents of blood donation motivation among non-donors of varying age and education.

    Science.gov (United States)

    Lemmens, K P H; Abraham, C; Ruiter, R A C; Veldhuizen, I J T; Dehing, C J G; Bos, A E R; Schaalma, H P

    2009-02-01

    Understanding blood donation motivation among non-donors is prerequisite to effective recruitment. Two studies explored the psychological antecedents of blood donation motivation and the generalisability of a model of donation motivation across groups differing in age and educational level. An older well-educated population and a younger less well-educated population were sampled. The studies assessed the role of altruism, fear of blood/needles and donation-specific cognitions including attitudes and normative beliefs derived from an extended theory of planned behaviour (TPB). Across both samples, results showed that affective attitude, subjective norm, descriptive norm, and moral norm were the most important correlates of blood donation intentions. Self-efficacy was more important among the younger less well-educated group. Altruism was related to donation motivation but only indirectly through moral norm. Similarly, fear of blood/needles only had an indirect effect on motivation through affective attitude and self-efficacy. Additional analyses with the combined data set found no age or education moderation effects, suggesting that this core model of donation-specific cognitions can be used to inform future practical interventions recruiting new blood donors in the general population. PMID:18547458

  3. BLOOD DONORS CAMPAIGN

    CERN Document Server

    2002-01-01

    Wednesday 13 November 2002 in restaurant nr 2, from 8.30 to 16.30 hrs will be held a blood donors campaign, organized by the Etablissement de Transfusion de Haute-Savoie If you already have a card giving your blood group, please bring this with you.

  4. BLOOD DONORS CAMPAIGN

    CERN Document Server

    2000-01-01

    A blood donors campaign, organized by the Établissement de Transfusion de Rhône-Alpes will be held at CERN on Tuesday 14 November 2000 in restaurant nr 2, from 8.30 to 16.30 hrs If you already have a card giving your blood group, please bring this with you.

  5. BLOOD DONORS CAMPAIGN

    CERN Multimedia

    2002-01-01

    Tuesday 19 March 2002 in restaurant nr 2, from 9.00 to 16.30 hrs A blood donors campaign, organized by the Centre de Transfusion sanguine of Geneva If you already have a card giving your blood group, please bring this with you.

  6. BLOOD DONORS CAMPAIGN

    CERN Document Server

    2001-01-01

    A blood donors campaign, organized by the Centre de Transfusion d'Annemasse will be held at CERN on Tuesday 14 November 2001 in restaurant nr 2, from 9.00 to 16.30 hrs If you already have a card giving your blood group, please bring this with you.

  7. BLOOD DONORS CAMPAIGN

    CERN Document Server

    2001-01-01

    A blood donors campaign, organized by the Centre de Transfusion Sanguine of Geneva will be held at CERN on Tuesday 13 March 2001 in restaurant nr 2, from 9.00 to 16.30 hrs If you already have a card giving your blood group, please bring this with you.

  8. Becoming a Blood Stem Cell Donor

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    Full Text Available ... Find out why Close Becoming a Blood Stem Cell Donor NCIcancertopics Subscribe Subscribed Unsubscribe 351 351 Loading... ... considered becoming a bone marrow or blood stem cell donor? Follow this true story of a former ...

  9. Becoming a Blood Stem Cell Donor

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    Full Text Available ... total__ Find out why Close Becoming a Blood Stem Cell Donor NCIcancertopics Subscribe Subscribed Unsubscribe 351 351 Loading... ... Ever considered becoming a bone marrow or blood stem cell donor? Follow this true story of a former ...

  10. Becoming a Blood Stem Cell Donor

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    Full Text Available ... Find out why Close Becoming a Blood Stem Cell Donor NCIcancertopics Subscribe Subscribed Unsubscribe 352 352 Loading... ... considered becoming a bone marrow or blood stem cell donor? Follow this true story of a former ...

  11. Becoming a Blood Stem Cell Donor

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    Full Text Available ... total__ Find out why Close Becoming a Blood Stem Cell Donor NCIcancertopics Subscribe Subscribed Unsubscribe 361 361 Loading... ... Ever considered becoming a bone marrow or blood stem cell donor? Follow this true story of a former ...

  12. Becoming a Blood Stem Cell Donor

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    Full Text Available ... total__ Find out why Close Becoming a Blood Stem Cell Donor NCIcancertopics Subscribe Subscribed Unsubscribe 350 350 Loading... ... Ever considered becoming a bone marrow or blood stem cell donor? Follow this true story of a former ...

  13. Hemodynamic Changes in Blood Donors

    Directory of Open Access Journals (Sweden)

    M Rafiei

    2004-07-01

    Full Text Available Introduction: Everyday, millions of people around the world go through phlebotomy, either to donate blood or for therapeutic intention. The most important worrisome adverse effects are hemodynamic alterations. In this study, hemodynamic changes following blood donation were assessed. Methods & Materials: Three hundred laborers who donated blood voluntarily were enrolled in this study. Blood pressure (BP and pulse rate were measured before the procedure, ten minutes afterwards, and one week following phlebotomy. Hemoglobin (Hgb and hematocrit (Hct were also determined prior to and one week after phlebotomy. Finally, results before and after donation were compared with each other. Results: 242 volunteers had normal BP and 58 were hypertensive. The mean systolic blood pressures (SBP before phlebotomy, ten minutes after the procedure, and one week later were 120, 117, and 122 mmHg, respectively. During the same periods of time, the mean of diastolic blood pressures (DBP were 77 , 78 and 78 mmHg , in order , while pulse rates on average were 80 , 82 and 76 beats/minute . None of the aforementioned changes were clinically significant. After one week, Hgb decreased by about 0.3 g/dl (P<0.001 and Hct declined on average of 1.7 (P<0.001. Forty six individuals had high DBP and one week after donation, their DBP was reduced by 7 mmHg. Age, body mass index and smoking did not have any significant effect on hemodynamic status. Conclusion: Hemodynamic changes in healthy blood donors were not clinically significant. It seems that DBP drops desirably in hypertensive individuals. This needs to be evaluated more carefully in future studies.

  14. Rare blood donors with irregular antibodies

    Directory of Open Access Journals (Sweden)

    Krga-Milanović Mirjana

    2013-01-01

    Full Text Available Introduction. Blood groups are inherited biological characteristics that do not change throughout life in healthy people. Blood groups represent antigens found on the surface of red blood cells. Kell blood group system consists of 31 antigens. Kell antigen (K is present in 0.2% of the population (the rare blood group. Cellano antigen is present in more than 99% (the high-frequency antigen. These antigens have a distinct ability to cause an immune response in the people after blood transfusion or pregnancy who, otherwise, did not have them before. Case Report. This paper presents a blood donor with a rare blood group, who was found to have an irregular antibody against red blood cells by indirect antiglobulin test. Further testing determined the specificity of antibody to be anti-Cellano. The detected antibody was found in high titers (1024 with erythrocyte phenotype Kell-Cellano+. The blood donor was found to have a rare blood group KellKell. This donor was excluded from further blood donation. It is difficult to find compatible blood for a person who has developed an antibody to the high-frequency antigen. The donor’s family members were tested and Cellano antigen was detected in her husband and child. A potential blood donor was not found among the family members. There was only one blood donor in the Register of blood donors who was compatible in the ABO and Kell blood group system. Conclusion. For the successful management of blood transfusion it is necessary to establish a unified national register of donors of rare blood groups and cooperate with the International Blood Group Reference Laboratory in Bristol with the database that registers donors of rare blood groups from around the world.

  15. Hemochromatosis: the new blood donor.

    Science.gov (United States)

    Leitman, Susan F

    2013-01-01

    Hereditary hemochromatosis (HH) due to homozygosity for the C282Y mutation in the HFE gene is a common inherited iron overload disorder in whites of northern European descent. Hepcidin deficiency, the hallmark of the disorder, leads to dysregulated intestinal iron absorption and progressive iron deposition in the liver, heart, skin, endocrine glands, and joints. Survival is normal if organ damage is prevented by early institution of phlebotomy therapy. HH arthropathy is the symptom most affecting quality of life and can be debilitating. Genotype screening in large population studies has shown that the clinical penetrance of C282Y homozygosity is highly variable and can be very low, with up to 50% of women and 20% of men showing a silent phenotype. Targeted population screening for the HFE C282Y mutation is not recommended at present, but might be reconsidered as a cost-effective approach to management if counseling and care were better organized and standardized. Referral of patients to the blood center for phlebotomy therapy and use of HH donor blood for transfusion standardizes treatment, minimizes treatment costs, and may benefit society as a whole. Physician practices should be amended such that HH subjects are more frequently referred to the blood center for therapy.

  16. Syphilis seroprevalence estimates of Santa Catarina blood donors in 2010

    Directory of Open Access Journals (Sweden)

    Aysla Marcelino Baião

    2014-04-01

    Full Text Available Introduction Knowledge of blood donor characteristics is essential to better guide clinical and serological screening for hemotherapy. The objective of this study was to determine the syphilis seroprevalence and the associated factors of blood donors in the State of Santa Catarina, Brazil. Methods This population-based study from the State of Santa Catarina used information obtained from blood donation records. We analyzed 83,396 blood donor records generated from donors who were considered eligible to donate between January and August 2010. The aim of the study was to estimate the syphilis seroprevalence and its relationship with educational level, age, gender, geographical region and having donated blood in the past 12 months. We used descriptive analyses and a Poisson regression to calculate the prevalence ratios for the variables of interest. Results We found a 0.14% overall seroprevalence and significant differences among the following: first-time blood donors (0.19% versus repeat donors (0.03% to 0.08%; low educational levels (0.30% versus medium and high educational levels (0.08% to 0.19%; and donors who did not report their residence (0.88% or age (6.94% versus those who did. Increased syphilis seroprevalence was also significantly associated with increased age. Conclusion High syphilis seroprevalence was associated with lower educational level, age, first-time donation and the failure to provide age or residence information.

  17. Becoming a Blood Stem Cell Donor

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    Full Text Available ... on Jul 19, 2011 Ever considered becoming a bone marrow or blood stem cell donor? Follow this true ... Institutes of Health Clinical Center in Bethesda, MD. Bone marrow transplantation (BMT) and peripheral blood stem cell transplantation ( ...

  18. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... on Jul 19, 2011 Ever considered becoming a bone marrow or blood stem cell donor? Follow this ... Institutes of Health Clinical Center in Bethesda, MD. Bone marrow transplantation (BMT) and peripheral blood stem cell ...

  19. Iron Status in Norwegian Blood Donors

    OpenAIRE

    Røsvik, Anne Synnøve

    2010-01-01

    Background and aims: Blood banks in Norway struggle to close the gap between need and supply of blood. The overall objective of this study was to investigate the iron status in blood donors. The aims of this thesis were first to compare the iron status in new blood donors in 1993-97 and in 2005-06, to describe possible changes in iron status. To describe the effect of four consecutive blood donations without iron supplementation for newly recruited donors was the second aim. Th...

  20. Questionnaire-Related Deferrals in Regular Blood Donors in Norway

    Directory of Open Access Journals (Sweden)

    Håkon Reikvam

    2012-01-01

    Full Text Available Voluntary donation is a key issue in transfusion medicine. To ensure the safety of blood transfusions, careful donor selection is important. Although new approaches to blood safety have dramatically reduced the risks for infectious contamination of blood components, the quality and the availability of blood components depend on the willingness to donate and the reliability of the information given by the donors about their own health, including risk behavior. As donors who are deferred by the blood bank will be less motivated to return for donation, it is important to reduce the number of deferrals. The aims of the present study were to investigate the reasons for deferral of registered donors coming to the blood bank for donation, in order to identify areas of importance for donor education—as these deferrals potentially could be avoided by better donor comprehension. Deferral related to testing of donors is not included in this study as these deferrals are dependent on laboratory results and cannot be indentified by questionnaire or interview. Data were collected from all blood donors in a period for 18 months who came for blood donation at a large university hospital in Norway. 1 163 of the 29 787 regular donors, who showed up for donation, were deferred (3.9%. The main reasons were intercurrent illness (n=182 (15.6%, skin ulcers (n=170 (14.6%, and risk behaviour (n=127 (10.9%. In a community, intercurrent illnesses, skin ulcers, and potential risk behavior are the most frequent reasons for deferral of regular donors. Strategized effort on donor education is needed, as “failure to donate” reduces donor motivation.

  1. Low hemoglobin deferral in blood donors

    OpenAIRE

    Mast, Alan E.

    2013-01-01

    Low hemoglobin deferral occurs in about 10% of attempted whole blood donations and commonly is a consequence of iron deficiency anemia. Pre-menopausal women often have iron deficiency anemia caused by menstruation and pregnancy and have low hemoglobin deferral on their first donation attempt. Frequent donors also develop iron deficiency and iron deficiency anemia because blood donation removes a large amount of iron from the donor and the 56-day minimum inter-donation interval for donors in t...

  2. Predictors of hemoglobin in Danish blood donors

    DEFF Research Database (Denmark)

    Kotzé, Sebastian R; Pedersen, Ole B; Petersen, Mikkel S;

    2015-01-01

    BACKGROUND: It is well known that blood donors are at increased risk of iron deficiency and subsequent development of iron deficiency anemia. We aimed to investigate the effect of factors influencing hemoglobin (Hb) levels. STUDY DESIGN AND METHODS: Initiated in 2010, the Danish Blood Donor Study...

  3. AN ANDROID APPLICATION FOR VOLUNTEER BLOOD DONORS

    Directory of Open Access Journals (Sweden)

    Sultan Turhan

    2015-05-01

    Full Text Available There is an expectation that the blood will always be there when it is really needed. Blood donor volunteers constitute the main supply source in an effective blood supply chain management. They feed blood stocks through their donation. In an emergency situation, if the stocks are insufficient, the only source of blood supply will be the people who come to the health center and donate the blood on a voluntary basis. It is certain that time is a very important component in such situation. For this reason, the health care center should call the nearest available donor in order to ensure to get the service as quickly as possible. A smart phone application is developed to facilitate the identification of the nearest available blood donor volunteer and the communication with him/her in the emergency situations where the blood can’t be supplied through the blood banks’ stocks. In this paper this application will be presented.

  4. AN ANDROID APPLICATION FOR VOLUNTEER BLOOD DONORS

    OpenAIRE

    Sultan Turhan

    2015-01-01

    There is an expectation that the blood will always be there when it is really needed. Blood donor volunteers constitute the main supply source in an effective blood supply chain management. They feed blood stocks through their donation. In an emergency situation, if the stocks are insufficient, the only source of blood supply will be the people who come to the health center and donate the blood on a voluntary basis. It is certain that time is a very important component in such ...

  5. Iron status of regular voluntary blood donors

    Directory of Open Access Journals (Sweden)

    Mahida Vilsu

    2008-01-01

    Full Text Available Background: Our blood bank is a regional blood transfusion centre, which accepts blood only from voluntary donors. Aim: The aim is to study iron status of regular voluntary donors who donated their blood at least twice in a year. Materials and Methods: Prior to blood donation, blood samples of 220 male and 30 female voluntary donors were collected. Control included 100 each male and female healthy individuals in the 18- to 60-year age group, who never donated blood and did not have any chronic infection. In the study and control groups, about 10% subjects consumed non-vegetarian diet. After investigation, 85 males and 56 females having haemoglobin (Hb levels above 12.5 g/dl were selected as controls. Donors were divided into ≤10, 11-20, 21-50 and> 50 blood donation categories. Majority of the donors in> 50 donation category donated blood four times in a year, whereas the remaining donors donated two to three times per year. Haematological parameters were measured on fully automatic haematology analyzer, serum iron and total iron-binding capacity (TIBC by biochemical methods, ferritin using ELISA kits and transferrin using immunoturbidometry kits. Iron/TIBC ratio x 100 gave percentage of transferrin saturation value. Statistical Analysis: Statistical evaluation was done by mean, standard deviation, pair t -test, χ2 and anova ( F -test. Results: Preliminary analysis revealed that there was no significant difference in the iron profile of vegetarian and non-vegetarian subjects or controls and the donors donating < 20 times. Significant increase or decrease was observed in mean values of various haematological and iron parameters in donors who donated blood for> 20 times ( P < 0.001, compared to controls. Anaemia, iron deficiency and depletion of iron stores were more prevalent in female donors ( P < 0.05 compared to males and especially in those male donors who donated their blood for more than 20 times. Conclusion: Regular voluntary blood

  6. RESULTS OF THE SPECIAL BLOOD DONOR DAY

    CERN Multimedia

    SC Unit

    2008-01-01

    Responding to the HUG (Hôpitaux Universitaires de Genève) hospitals’ urgent appeal for blood donations during this summer season, the CERN medical staff organised a day of blood donations for the Swiss bloodbank CTS on 30 July. They were supported by NOVAE (Restaurant No. 1), who provided donors with a free snack. This specially arranged campaign was a success, as the 135 volunteers included 66 first-time donors, and a total of 99 standard bags of blood was collected. (Swiss hospitals need 1300 bags every day!) The CTS and CERN’s medical staff want to thank the donors and all others who helped make the event a success. Upcoming blood donor days at CERN: 12 November 2008 and 10 March 2009.

  7. RESULTS OF THE SPECIAL BLOOD DONOR DAY

    CERN Document Server

    SC Unit

    2008-01-01

    Responding to the HUG (Hôpitaux Universitaires de Genève) hospitals’ urgent appeal for blood donations during this summer season, the CERN medical staff organised a day of blood donations for the Swiss bloodbank CTS on 30 July. They were supported by NOVAE (Restaurant No. 1), who provided donors with a free snack. This specially arranged campaign was a success, as the 135 volunteers included 66 first-time donors, and a total of 99 standard bags of blood were collected. (Swiss hospitals need 1300 bags every day!) The CTS and CERN’s medical staff wish to thank the donors and all others who helped make the event a success. Upcoming blood donor days at CERN: 12 November 2008 and 10 March 2009.

  8. Becoming a Blood Stem Cell Donor

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    Full Text Available ... Queue __count__/__total__ Find out why Close Becoming a Blood Stem Cell Donor NCIcancertopics Subscribe Subscribed Unsubscribe ... later? Sign in to add this video to a playlist. Sign in Share More Report Need to ...

  9. How to Motivate Whole Blood Donors to Become Plasma Donors

    Directory of Open Access Journals (Sweden)

    Gaston Godin

    2014-01-01

    Full Text Available This study tested the efficacy of interventions to recruit new plasma donors among whole blood donors. A sample of 924 donors was randomized to one of three conditions: control; information only by nurse; and information plus self-positive image message by nurse (SPI. Participants in the control condition only received a leaflet describing the plasma donation procedure. In the two experimental conditions the leaflet was explained face-to-face by a nurse. The dependent variables were the proportion of new plasma donors and the number of donations at six months. Overall, 141 (15.3% new plasma donors were recruited at six months. There were higher proportions of new plasma donors in the two experimental conditions compared to the control condition (P<.001; the two experimental conditions did not differ. Also, compared to the control condition, those in the experimental conditions (all Ps<.001 gave plasma more often (information only by nurse:  d=.26; SPI: d=.32; the SPI intervention significantly outperformed (P<.05 the information only by nurse condition. The results suggest that references to feelings of SPI such as feeling good and being proud and that giving plasma is a rewarding personal experience favor a higher frequency of plasma donation.

  10. Potential donor segregation to promote blood donation.

    Science.gov (United States)

    Martín-Santana, Josefa D; Beerli-Palacio, Asunción

    2008-04-01

    This work is set in the field of social marketing and more specifically in the context of blood donation. Its principal objective focuses on segregating potential donors by using the inhibitors or barriers to a blood donation behaviour as criteria. Moreover, an analysis of the predisposition to donate blood, the intrinsic and extrinsic motivations for donating blood, and the incentives that may stimulate their donation conduct was conducted for each of the four identified groups. The results reveal that the four segments differ significantly in their predisposition to donate, in their motivations and in the incentives that encourage them to donate blood. PMID:18343199

  11. Hemochromatosis Patients as Voluntary Blood Donors

    Directory of Open Access Journals (Sweden)

    Tara E Power

    2004-01-01

    Full Text Available The present study was designed to investigate hemochromatosis patients' suitability as blood donors as well as their perceptions and experience with the current public donation system. Participants were gathered from a list of current hemochromatosis patients (n=120 and members of the Canadian Hemochromatosis Society (n=1000. Of the 1120 surveys mailed out to these groups, 801 surveys were returned completed. The sample respondents had a mean age of 57.44 years (SD=12.73; range 19 to 87 years, and 57% were men. It was found that 20% (160 of the respondents have donated blood since their diagnosis; however, only 12% of the respondents indicated that they use voluntary blood donation as a means of maintaining their iron levels. Forty per cent of the respondents indicated that they had been refused from voluntary donation. Despite the fact that in May 2001 the Canadian Blood Services, in collaboration with the Canadian Hemochromatosis Society, began a promotion campaign to encourage hemochromatosis patients to become voluntary blood donors, the present study found that 15% of the respondents reported having been refused from the voluntary blood donation service due to the diagnosis of hemochromatosis. With respect to quality of life, it was found that individuals who donate blood were generally healthier with respect to physical functioning and bodily pain, however, these findings may indicate that hemochromatosis patients who are healthier are better able to donate at public blood banks, rather than that voluntary blood donation has an effect on the donors' physical functioning over phlebotomy clinic users. These study findings suggest that although there may be other medical factors limiting individuals from donating, hemochromatosis patients are interested in being voluntary blood donors and this potential resource is currently under-used.

  12. Lipid profile of regular blood donors

    Directory of Open Access Journals (Sweden)

    Uche EI

    2013-05-01

    Full Text Available EI Uche,1 A Adediran,2 OD Damulak,3 TA Adeyemo,2 AA Akinbami,4 AS Akanmu21Department of Hematology and Blood Transfusion, Lagos University Teaching Hospital, Lagos, Nigeria; 2Department of Hematology and Blood Transfusion, University of Lagos, Lagos, Nigeria; 3Department of Hematology and Blood Transfusion, Jos University Teaching Hospital, Jos, Nigeria; 4Department of Hematology and Blood Transfusion, Lagos State University, Ikeja, NigeriaIntroduction: A few reports have linked regular blood donation to the lowering of parameters of lipid profile. Estimating the lipid profile is an accepted method of assessing an individual’s risk for coronary heart disease, particularly if there is evidence of lipid peroxidation. Regular blood donation may lower iron stores, and this in turn lowers lipid peroxidation. This study was carried out to determine the effect of blood donation on lipid profile.Materials and methods: Eighty-two participants consented to participate and were enrolled into the study, 52 of whom were regular blood donors (study group and 30 were non-donors (control group. Venous blood (10 mL was drawn from each subject into new plain screw-capped disposable plastic tubes. This was allowed to clot and the serum was used to determine total cholesterol, triglycerides, low-density lipoprotein, and high-density lipoprotein.Results: The mean total cholesterol (4.66 ± 0.86 mmol/L, triglycerides (1.22 ± 0.64 mmol/L, and low-density lipoprotein (2.32 ± 0.73 mmol/L were significantly lower in the regular blood donors than the control group (5.61 ± 1.26 mmol/L, 1.77 ± 2.9 mmol/L, and 3.06 ± 0.89 mmol/L, respectively; P < 0.05 in all cases. Also, while 42% of the study group had a low/high-density lipoprotein ratio of at least three, about 57% of the control group had a ratio of at least three (P = 0.21.Conclusion: Regular blood donation may be protective against cardiovascular disease as reflected by significantly lower mean total

  13. Blood donation and blood donor mortality after adjustment for a healthy donor effect

    DEFF Research Database (Denmark)

    Ullum, Henrik; Rostgaard, Klaus; Kamper-Jørgensen, Mads;

    2015-01-01

    BACKGROUND: Studies have repeatedly demonstrated that blood donors experience lower mortality than the general population. While this may suggest a beneficial effect of blood donation, it may also reflect the selection of healthy persons into the donor population. To overcome this bias, we...... investigated the relation between blood donation frequency and mortality within a large cohort of blood donors. In addition, our analyses also took into consideration the effects of presumed health differences linked to donation behavior. STUDY DESIGN AND METHODS: Using the Scandinavian Donation and...... and mortality. The magnitude of the association was reduced after adjustment for an estimate of self-selection in the donor population. Our observations indicate that repeated blood donation is not associated with premature death, but cannot be interpreted as conclusive evidence of a beneficial health...

  14. [Presence of Australia antigen in blood donors].

    Science.gov (United States)

    Gota, F

    1980-01-01

    The differential diagnosis of type A and B viral hepatitis is discussed and guidelines for the prevention of post-transfusional hospital hepatitis are proposed. Methods for the immunological demonstration of HBs antigen are illustrated, together with the respective positivity percentages in blood donors.

  15. Becoming a Blood Stem Cell Donor

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    Full Text Available ... blood stem cell (PBSC) donor, explains the donation process - Duration: 3:28. Be The Match 23,393 ... Copyright Creators Advertise Developers +YouTube Terms Privacy Policy & Safety Send feedback Try something new! Loading... Working... Sign ...

  16. Becoming a Blood Stem Cell Donor

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    Full Text Available ... be donors at http://www.marrow.org . Category Science & Technology License Standard YouTube License Show more Show ... Monks 3,700 views 4:41 Stem Cell Basics - How Blood is Made. - Duration: 10:58. Vernon ...

  17. Alternative donors: cord blood for adults.

    Science.gov (United States)

    Ruggeri, Annalisa

    2016-04-01

    Allogeneic hematopoietic stem cell transplantation (HSCT) is a curative treatment for patients with hematological diseases. The probability of finding a human leukocyte antigen (HLA)- identical donor among family members is around 25% and 30% that of having a full matched unrelated donor in the registry. Patients in need may also benefit of a HLA-mismatched HSCT either from an haploidentical donors or from umbilical cord blood (UCB). Much has been learned about UCB transplant (UCBT) since the first human UCBT was performed back in 1988. Cord blood banks have been established worldwide for the collection, cryopreservation, and distribution of UCB for HSCT. Today, a global network of cord blood banks and transplant centers has been established with a large common inventory of more than 650,000 UCB units available, allowing for more than 40,000 UCBT worldwide in children and adults with severe hematological diseases. Several studies have been published on UCBT, assessing risk factors such as cell dose and HLA mismatch. Outcomes of several retrospective comparative studies showed similar results using other stem cell sources both in pediatric and adult setting. New strategies are ongoing to facilitate engraftment and reduce transplant-related mortality. In this issue, we review the current results of UCBT in adults with hematological malignancies and the clinical studies comparing UCBT with other transplant strategies. We provide guidelines for donor algorithm selection in UCBT setting.

  18. Hemochromatosis Patients as Voluntary Blood Donors

    OpenAIRE

    Power, Tara E.; Paul C Adams

    2004-01-01

    The present study was designed to investigate hemochromatosis patients' suitability as blood donors as well as their perceptions and experience with the current public donation system. Participants were gathered from a list of current hemochromatosis patients (n=120) and members of the Canadian Hemochromatosis Society (n=1000). Of the 1120 surveys mailed out to these groups, 801 surveys were returned completed. The sample respondents had a mean age of 57.44 years (SD=12.73; range 19 to 87 yea...

  19. The evaluation of blood donor deferral causes in Zimbabwe

    NARCIS (Netherlands)

    Mafirakureva, N.; Khoza, S.; Van Hulst, M.; Postma, M.J.; Mvere, D.A.

    2013-01-01

    Background: Donor selection is one of the first steps in blood donation aimed at improving the safety of blood and blood products. It is the only line of protection for donors and against certain infections for which testing is not performed. There is paucity of published data on reasons for donor d

  20. Prevalence Of Hepatitis C Antibodies In Healthy Blood Donors

    OpenAIRE

    Gupta Nalini; Kumar Amarjit

    2002-01-01

    Research question : What is the prevalence of hepatitis C virus infection in healthy blood donors? Objective: To screen the blood donors for HCV antibodies routinely. Study design: Cross- sectional. Setting: Deptt. of Transfusion Medicine, Dayanand Medical College & Hospital, Ludhiana. Participants: Healthy blood donors. Statistical analysis: Prevalence rate. Results: Prevalence of anti HCV was found to be 1.5%, which is quite high.

  1. Recruitment of prospective donors: what do they expect from a homepage of a blood transfusion service?

    Science.gov (United States)

    Moog, R; Fourné, K

    2007-08-01

    In times of shrinking donor population, the recruitment of donors is of utmost importance. Recruitment can be done by personal communication, advertisement/information, classical mass media (newspaper, radio, TV) or new computerized media. The aim of this study was to gain information about the donors' demands of an Internet presentation of a blood transfusion service. Between October and December 2004 inclusive, prospective donors were asked to complete a survey about the impact of Internet information for blood donors. The survey contained questions measuring demographics, education and motivation for blood donation. In addition, the survey included questions that measured Internet access, duration of online time and donors' demands for an Internet presentation of a blood transfusion service. Donors were asked to tick a box with predefined answers. In cases where no options were applied, donors were requested to specify their answers. One hundred and fourteen prospective donors (71 female, 43 male) with a median age of 25 years (range 18-57 years) completed the survey. Most donors (57.9%) were 18-30 years old. Forty-two (36.8%) of the surveyed donors were repeat donors, whereas 72 (63.2%) were first-time donors. The majority of donors were informed about blood donation from relatives or friends (70.7% repeat donors and 67.7% first-time donors). Most of them had Internet access (85.7% repeat donors and 90.3% first-time donors). Exclusive use of private access was more often reported in repeat donors (77.8%), whereas both private and professional access was more frequently used in first-time donors (32.3%). Most donors used the Internet access daily, followed by weekly and monthly use. Multiple answers were given about the importance of desired information about the topic 'blood donation'. Both first-time and repeat donors wanted to be informed about organizational details of blood donation such as opening times, eligibility criteria, donation process and the kind

  2. Occult HCV in Egyptian volunteer blood donors

    Directory of Open Access Journals (Sweden)

    Mostafa A. Amin 1, Kouka S. E. Abdel-Wahab 2 and Adel A.

    2013-01-01

    Full Text Available Objective: The study aims to investigate the risk of post-transfusion transmission of hepatitis c virus (HCV in the circumstances of occult HCV when anti-HCV is undetectable by ELISA and HCV-RNA is detected by RT-PCR in the plasma and or in peripheral blood mononuclear cells (PBMCs of donor blood and the recipients are immunocompromised. Patients & methods: The study covered 18 chronic renal failure patients (CRF [12 males (66.7% their age ranged from 28 to 65 years and 6 females (33.3 % their age ranged from 15 to 55 years] undergoing hemodialysis in Nile Hospital as part of their therapy have to receive blood transfusions (275 blood units for the first time. Commercial ELISA kits for anti-HCV and nested-RT-PCR (N-RT-PCR kits were used. Results: Anti-HCV was positive in one serum from the eighteen (5.5% poly transfused CRF patients at the end of the study while the seventeen sera were negative. This serum was also positive for HCV RNA by N-RT-PCR. Out of the 20 transfused blood units, one blood unit (three components were tested by blood banking anti-HCV negative by ELISA, were positive for HCV RNA by N-RT-PCR. The collective markers of this blood unit represent an occult HCV. The risk of acquiring post-transfusion HCV infection from an occult HCV blood unit is 5%. Real time PCR showed variation in the viral load of the serum of the infected CRF patient, the plasma of blood unit, the PBMCs of this blood unit whether activated by PHA-M or not.

  3. Prevalence of seroreactivity among blood donors in rural population.

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    Sonwane B

    2003-09-01

    Full Text Available The seroreactivity pattern amongst blood donors in rural population was studied at S.R.T. Rural Medical College and Hospital, Ambajogai (M. S.. The study period was from January 1996 to December 2001. A total number of 12,240 blood donors were screened. The voluntary donation was 36.98% and replacement donors were 63.02%. No professional donor is bled in our blood bank. The HIV seroreactivity among voluntary donors was 1.56% and 2.11% in replacement donors. The HBsAg seroreactivity was 2.78% in voluntary donors and 4.84% in replacement donors. VDRL seroreactivity is 1.12% in replacement donors. No malarial parasite and HCV seroreactive donor was found in our study period. We have found the magnitude of hepatitis to be far more than that of HIV. Hence testing for HCV routinely is mandatory, besides HBsAg.

  4. Asymptomatic Malaria among Blood Donors in Benin City Nigeria.

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    Bankole Henry Oladeinde

    2014-09-01

    Full Text Available This study aimed at determining the prevalence and associated risk factors for asymptomatic malaria parasitaemia and anemia among blood donors in a private medical laboratory in Benin City, Nigeria.Venous blood was collected from a total of 247 blood donors. Malaria status, ABO, Rhesus blood groups and hemoglobin concentration of all participants were determined using standard methods.The prevalence of asymptomatic malaria infection was higher among commercial blood donors than volunteer group (commercial vs volunteer donor: 27.5 %vs. 13.8%; OR = 2.373, 95% CI = 0.793, 7.107, P = 0.174. Asymptomatic malaria was not significantly affected by gender (P = 0.733, age (P = 0.581, ABO (P = 0.433 and rhesus blood groups (P = 0.806 of blood donors. Age was observed to significantly (P = 0.015 affect malaria parasite density with donors within the age group of 21-26 years having the highest risk. The prevalence of anemia was significantly higher among commercial donors (commercial vs volunteer donors: 23.4% vs 3.4%: OR = 8.551, 95% CI = 1.135, 64.437, P = 0.013 and donors of blood group O type (P = < 0.0001.Asymptomatic malaria parasitaemia and anemia was higher among commercial donors than voluntary donors. Mandatory screening of blood donors for malaria parasite is advocated to curb transfusion transmitted malaria and associated sequelae.

  5. PSYCHOSOCIAL VARIABLES OF HIGHLY MOTIVATED VOLUNTARY BLOOD DONORS AT BLOOD BANK OF A MEDICAL COLLEGE

    OpenAIRE

    Bhalodia Jignasa N; Patel Kinara A; Oza Hiren V

    2012-01-01

    Background: The adequacy of blood depends on blood donation rates and numbers of blood donors. To prepare adequate blood supplies, it is essential to investigate the factors that motivate individuals from donating. This study aimed to identify the character of highly motivated donors. This study was undertaken to study the motivational factors leading to voluntary blood donation and understanding the psychosocial variables of blood donors. Methodology: We selected 50 regular blood donors who ...

  6. Prevalence of malaria parasites among blood donors in Kaduna, Nigeria

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    Douglas D. Garba

    2016-06-01

    Conclusions: The prevalence of malaria parasites among blood donors was 7.5% Blood donors should be routinely screened for malaria parasites and the blood marked negative or positive as the case may be. Recipients of malaria parasites positive blood should be given prophylactic treatment to prevent transfusion related malaria (TRM. [Int J Res Med Sci 2016; 4(6.000: 2112-2119

  7. DONOR DEFERRAL PATTERN AMONG BLOOD DONORS IN BLOOD BANK OF A MEDICAL COLLEGE HOSPITAL OF CHHATTISGARH

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    Bhanu P .

    2015-09-01

    Full Text Available A 3 Years retrospective study conducted on “Donor Deferral Pattern among Blood Donors in Blood Bank’’ of a Medical College Hospital of Chhattisgarh. This study shows the major possible causes of deferrals for donation of blood in northern Chhattisgarh. Donor’s detail information on the donor deferral including the cause of deferral was recorded in deferral register. Donors deferred were analyzed and computed for study on the bas ic of medical examination. In the study done f ro m A ugust 2012 to July 2015 a total of 18773 donors were registered and the screened. Out of these 18153 were male and 620 were female. Among the male, 1486 were deferred which makes 8.18% of the total registe red. Among female, 339 were deferred which makes 54.67% of the total registered. The maximum numbers of deferrals were due to low Hb (17.97% the second most important cause for deferral in this region happens to be alcohol/ganja intake (11.61%. The third leading cause is skin puncture or ear piercing (10.57%. This figure is mainly due to professional donors who frequent around the hospital very often. Interestingly forth cause is deferral is medication (7.39% mostly over the counter drugs. Other major c ause for deferrals in descending order are low BP (6.68%, fever and cold (6.30%, hypertension (5.64%, underweight (4.54%, Typhoid (4.05%, menstrual cycle (3.39% and donated <3 month (2.41%. The least percentage of cause of deferral was due to breast feeding (0.10%. It is because of very low percentage of female donation. Strict screening of donor is compulsory to achieve safe blood equally important is to increase the number of voluntary donations with minimum deferral. To achieve this it is necessa ry to study and analyze various causes for donor deferral & categorize them under temporary & Permanent, so that temporary deferral can be converted to donations. Proper medical examination and Strict deferral system of the blood donors in blood banks redu ces the

  8. Questionnaire-Related Deferrals in Regular Blood Donors in Norway

    OpenAIRE

    Håkon Reikvam; Kjersti Svendheim; Anne S. Røsvik; Tor Hervig

    2012-01-01

    Voluntary donation is a key issue in transfusion medicine. To ensure the safety of blood transfusions, careful donor selection is important. Although new approaches to blood safety have dramatically reduced the risks for infectious contamination of blood components, the quality and the availability of blood components depend on the willingness to donate and the reliability of the information given by the donors about their own health, including risk behavior. As donors who are deferred by the...

  9. Risk Factors of Viral Hepatitis B among Egyptian Blood Donors

    OpenAIRE

    Awadalla, HI; Ragab, MH; Osman, MA; Nassar, NA; Cairo, Egypt.

    2011-01-01

    Background: Surveillance of infectious disease markers in the blood donor population is important in recognizing trends in prevalence and incidence of transfusion related infections in asymptomatic volunteer blood donors. Subjects & Methods: It is a community base cross sectional study. Subjects of study are volunteers to donate blood. Samples were collected from blood donors and questionnaire was designed to collect the risk factors data. The prevalence of hepatitis B surface antigen (HB...

  10. Blood donors screening for blood born viruses in Poland.

    Science.gov (United States)

    Grabarczyk, Piotr; Kopacz, Aneta; Sulkowska, Ewa; Kubicka-Russel, Dorota; Mikulska, Maria; Brojer, Ewa; Łętowska, Magdalena

    2015-01-01

    Blood donor screening of viral markers in Poland is based on serologic testing for anti-HCV, HBsAg, anti-HIV1/2 (chemiluminescence tests) and on nucleic acid testing (NAT) for RNA HCV, RNA HIV-1 and DNA HBV performed in minipools of 6 with real-time PCR (MPX 2.0 test on cobas s201) or with TMA in individual donations (Ultrio Plus or Ultrio Elite). Donors of plasma for anti-D and anti-HBs production are tested for parvovirus B19 DNA. Before implementation tests and equipment are evaluated at the Institute of Hematology and Transfusion Medicine (IHTM). The last 20 years witnessed a decreasing trend for HBsAg in both first time and repeat donors (1%-0.3% and 0.1%-0.02% respectively). Prevalence of anti-HCV repeat reactive results was stable and oscillated around 0.8% for first time donors and 0.2% for repeat donors. Elevated prevalence of seropositive HIV infected donors was recently observed (7.5-9 cases/100,000 donors). Since respective molecular markers implementation HCV RNA was detected on average in 1/119,235 seronegative donations, HIV RNA in 1/783,821 and HBV DNA in 1/61,047. HBV NAT yields were mostly occult hepatitis B (1/80,248); window period cases were less frequent (1/255,146). The efficiency of HBV DNA detection depends on the sensitivity of the HBV DNA screening system. PMID:26519842

  11. Eligibility and Exclusion of Hemochromatosis Patients as Voluntary Blood Donors

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    M Levstik

    1998-01-01

    Full Text Available BACKGROUND: Hereditary hemochromatosis patients are excluded in many countries as voluntary blood donors. In 1991, changes in the Canadian Red Cross policy allowed healthy hemochromatosis patients to become voluntary donors.

  12. Characteristics of blood donors and factors associated with blood donation in Guangzhou

    OpenAIRE

    Ouyang, Jian; 欧阳剑

    2013-01-01

    Objective: To describe and compare the characteristics of blood donors and non-donors and to examine factors associated with donation, including motivators and barriers of blood donation in Guangzhou, China. Design: Cross-sectional survey using self-administered standardized structured questionnaires on both donors and non-donors. Setting: 12 mobile and 4 permanent blood donation stations in Guangzhou during the whole operation time. Participants: 500 blood donors who donated at th...

  13. Hepatitis C virus infection in the asymptomatic British blood donor.

    Science.gov (United States)

    Mutimer, D J; Harrison, R F; O'Donnell, K B; Shaw, J; Martin, B A; Atrah, H; Ala, F A; Skidmore, S; Hubscher, S G; Neuberger, J M

    1995-01-01

    Blood donor screening for hepatitis C virus (HCV) antibodies is now routine. Most blood transfusion services recommend that seropositive donors are referred for further investigation. Southern European studies suggest that many asymptomatic seropositive donors have clinically significant liver disease. Seropositive donors in areas of high prevalence may not, however, be representative of British donors. We have prospectively examined the prevalence and severity of HCV infection in a British volunteer blood donor population. During a 14 month period, only 0.35% (999/287,332) of all donors in the West Midlands were anti-HCV (screening assay) positive. Only 5% (52/999) of these were confirmed true seropositive. Nearly 80% (41/52) of seropositive donors were referred to the Queen Elizabeth Hospital Liver Unit for further investigation. Most underwent complete investigation, including liver biopsy. Forty of forty-one donors had biochemical, histological, or virological evidence of persistent viral infection. Histological changes were generally mild and none was cirrhotic. Covertly infected patients had less severe disease than those with an overt risk factor for HCV exposure. In the British Midlands, the prevalence of blood donor seropositivity is low. In contrast with seropositive Southern European donors, the British donor is more likely to belong to an at-risk group for parenteral exposure and is less likely to have severe histological changes. This study highlights the importance of developing locally relevant guidelines for the counselling and investigation of anti-HCV-positive blood donors. PMID:7493294

  14. Blood group antigen distribution in Lao blood donors.

    Science.gov (United States)

    Keokhamphoui, C; Urwijitaroon, Y; Kongphaly, D; Thammavong, T

    2012-01-01

    Blood group antigens can be distributed differently within different nationalities. Therefore, information about the prevalence of blood group antigens in the Lao population will be useful for providing better blood transfusion services in the Lao People's Democratic Republic. The purpose of this study was to determine the prevalence of blood group antigens in Lao blood donors. Blood samples from 464 Lao national volunteer blood donors were typed for antigens in various blood group systems including ABO, MNS, P1PK, Rh, Kell, Lewis, Duffy, Kidd, and Diego. The results show similar antigen prevalence to that among Northeast Thais for ABO, MNS, P1PK, Rh, Kell, and Duffy systems. In the ABO system, 0 was the highest at 37.72 percent,followed by 35.56 percent B, 19.83 percent A1, 6.47 percent A1B,and 0.43 percent A2B. The common phenotypes were D+C+E-ce+at 60.43 percent, M+N-S-s+ at 46.55 percent, Fy(a+b-) at 80.82 percent, Jk(a+b+) at 39.44 percent, and kk at 99.72 percent.Interestingly, Le(a-b-) was found at 50.43 percent, which was significantly higher than previous reports in Thais and Taiwanese.The P1 antigen was found in only 18.97 percent, which is much lower than in Whites and Blacks. Rare phenotypes were Fy(a-b+)and Jk(a-b-), found at 0.22 percent and 4.31 percent, respectively.In terms of negative antigens the study shows 0.22 percent Fy(a-), 35.34 percent Jk(a-), 29.53 percent Jk(b-), 3.04 percent C-, 2.39 percent e-, and 5.17 percent M-. The high prevalence of C, e, and Fy" and immunogenicity of these antigens may induce alloimmunization in transfusion-dependent patients, creating difficulties providing blood from Lao donors. The information obtained from this study will be useful for improving transfusion therapy in the country, especially for estimation of the availability of compatible blood for patients who have produced antibodies. PMID:23421543

  15. Prevalence Of Hepatitis C Antibodies In Healthy Blood Donors

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    Gupta Nalini

    2002-01-01

    Full Text Available Research question : What is the prevalence of hepatitis C virus infection in healthy blood donors? Objective: To screen the blood donors for HCV antibodies routinely. Study design: Cross- sectional. Setting: Deptt. of Transfusion Medicine, Dayanand Medical College & Hospital, Ludhiana. Participants: Healthy blood donors. Statistical analysis: Prevalence rate. Results: Prevalence of anti HCV was found to be 1.5%, which is quite high.

  16. Radioimmunoassay of serum β2-microglobulin in donor's blood

    International Nuclear Information System (INIS)

    Serum β2-microglobulin (β2-MG) was tested by radioimmunoassay in 149 donors' and 54 healthy volunteers' blood. The results were 203 +- 33.0 nmol/l and 176 +- 26.2 nmol/l, respectively. There was significant difference statistically between them (P2-MG content. In order to increase the quality of donated blood and to keep the health of blood donor, it is suggested that the high content of serum β2-MG is the indicator of too frequent blood donating. The results also showed that the content of β2-MG in donor's blood is not a normal reference value

  17. Seroepidemiology of infection with Toxoplasma gondii in healthy blood donors of Durango, Mexico

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    Estrada-Martínez Sergio

    2007-07-01

    Full Text Available Abstract Background Toxoplasma gondii (T. gondii infection in blood donors could represent a risk for transmission in blood recipients. There is scarce information about the epidemiology of T. gondii infection in blood donors in Mexico. Therefore, we sought to determine the prevalence of T. gondii infection and associated socio-demographic and behavioral characteristics in a population of healthy blood donors of Durango City, Mexico. Methods Four hundred and thirty two blood donors in two public blood banks of Durango City, Mexico were examined for T. gondii infection between August to September 2006. Blood donors were tested for anti-T. gondii IgG and IgM antibodies by using enzyme-linked immunoassays (Diagnostic Automation Inc., Calabasas, CA, USA. Socio-demographic and behavioral characteristics from each participant were also obtained. Results Thirty two (7.4% of 432 blood donors had IgG anti-T. gondii antibodies. Eight (1.9% of them had also IgM anti-T. gondii antibodies. Multivariate analysis using logic regression showed that T. gondii infection was associated with the presence of cats at home (adjusted OR = 3.81; 95% CI: 1.45–10.01. The age group of 45–60 years showed a significantly higher frequency of T. gondii infection than the group of 25–34 years (p = 0.02. Blood donors without education had a significantly higher frequency of infection (15.8% than those with 13–19 years of education (4.5% (p = 0.04. Other characteristics of blood donors including male gender, consumption of undercooked meat or blood transfusion did not show an association with infection. Conclusion The prevalence of T. gondii infection in healthy blood donors of Durango City, Mexico is lower than those reported in blood donors of south and central Mexico, and is one of the lowest reported in blood donors worldwide. T. gondii infection in our blood donors was most likely acquired by contact with cats. Prevalence of infection increased with age and decreased

  18. Bartonella spp. bacteremia in blood donors from Campinas, Brazil.

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    Luiza Helena Urso Pitassi

    2015-01-01

    Full Text Available Bartonella species are blood-borne, re-emerging organisms, capable of causing prolonged infection with diverse disease manifestations, from asymptomatic bacteremia to chronic debilitating disease and death. This pathogen can survive for over a month in stored blood. However, its prevalence among blood donors is unknown, and screening of blood supplies for this pathogen is not routinely performed. We investigated Bartonella spp. prevalence in 500 blood donors from Campinas, Brazil, based on a cross-sectional design. Blood samples were inoculated into an enrichment liquid growth medium and sub-inoculated onto blood agar. Liquid culture samples and Gram-negative isolates were tested using a genus specific ITS PCR with amplicons sequenced for species identification. Bartonella henselae and Bartonella quintana antibodies were assayed by indirect immunofluorescence. B. henselae was isolated from six donors (1.2%. Sixteen donors (3.2% were Bartonella-PCR positive after culture in liquid or on solid media, with 15 donors infected with B. henselae and one donor infected with Bartonella clarridgeiae. Antibodies against B. henselae or B. quintana were found in 16% and 32% of 500 blood donors, respectively. Serology was not associated with infection, with only three of 16 Bartonella-infected subjects seropositive for B. henselae or B. quintana. Bartonella DNA was present in the bloodstream of approximately one out of 30 donors from a major blood bank in South America. Negative serology does not rule out Bartonella spp. infection in healthy subjects. Using a combination of liquid and solid cultures, PCR, and DNA sequencing, this study documents for the first time that Bartonella spp. bacteremia occurs in asymptomatic blood donors. Our findings support further evaluation of Bartonella spp. transmission which can occur through blood transfusions.

  19. Geographical variations in the correlates of blood donor turnout rates: An investigation of Canadian metropolitan areas

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    Newbold K Bruce

    2009-10-01

    Full Text Available Abstract Background Like other countries, Canada's population is aging, and the implications of this demographic change need to be better understood from the perspective of blood supply. Analysis of donor data will help to identify systematic patterns of donation and its correlates. Data Geo-coded blood donor and donor clinic data are provided by Canadian Blood Services. Blood donor data is provided for the fiscal year 2006-2007 indicating the total number of donors for each Canadian postal code, excluding the province of Québec. Potential correlates of blood donation are selected based on social and economic characteristics, as well as descriptors of city size and geographical location in the urban hierarchy measures of accessibility, and capacity of donor clinics. Methods Data is aggregated to n = 3,746 census tracts in 40 Census Metropolitan Areas (CMA across the country. The number of donors per population in a census tract is regressed against the set of potential donation correlates. Autocorrelation is tested for and results adjusted to provide parsimonious models. Results A number of factors are found to influence donation across the country, including the proportion of younger residents, English ability, proportion of people with immigrant status, higher education, and a population-based measure of accessibility. Conclusion While a number of correlates of blood donation are observed across Canada, important contextual effects across metropolitan areas are highlighted. The paper concludes by looking at policy options that are aimed toward further understanding donor behaviour.

  20. Frequency of West Nile Virus Infection in Iranian Blood Donors.

    Science.gov (United States)

    Aghaie, Afsaneh; Aaskov, John; Chinikar, Sadegh; Niedrig, Matthias; Banazadeh, Soudabeh; Mohammadpour, Hashem Khorsand

    2016-09-01

    West Nile virus (WNV) can be transmitted by blood transfusions and organ transplants. This study was a retrospective study which was performed in Blood Transfusion Center to evaluate the WNV infection in blood donors in Iran. A total of 540 blood samples were taken from volunteer healthy donors who referred for blood donation to Chabahar Blood Center. The presence of WNV was studied by detecting immunoglobulin G (IgG) WNV by enzyme linked immune sorbent assay (ELISA). Demonstration of elevated WNV IgG confirmed by immunoflouorescence assay (IFA) Euroimmun kit. Out of the 540 samples 17.96 % (97 cases) were seropositive by ELISA and 1.48 % (8 cases) was seropositive by IFA. This means that 8.24 % of ELISA seropositive samples were confirmed by IFA. Special attention should be paid to criteria of donor selection, albeit positive results may be due to a previous infection in these donors. PMID:27429528

  1. Characterization of blood donors with high haemoglobin concentration

    DEFF Research Database (Denmark)

    Magnussen, K; Hasselbalch, H C; Ullum, H;

    2013-01-01

    Background and Objectives  The literature contains little on the prevalence and causes of high predonation haemoglobin levels among blood donors. This study aimed to characterize and develop an algorithm to manage would-be donors with polycythaemia. Materials and Methods  Between November 2009...... and November 2011, we offered haematology consultations to blood donors with repeated haemoglobin concentration (Hb) above the WHO limit for polycythaemia vera (PV) (10·2 and 11·5 mm/16·5 and 18·5 g/dl for women and men, respectively). Investigation of such donors included Hb, haematocrit, mean cell volume...

  2. Eligibility and Exclusion of Hemochromatosis Patients as Voluntary Blood Donors

    OpenAIRE

    Levstik, M; Adams, PC

    1998-01-01

    BACKGROUND: Hereditary hemochromatosis patients are excluded in many countries as voluntary blood donors. In 1991, changes in the Canadian Red Cross policy allowed healthy hemochromatosis patients to become voluntary donors.STUDY DESIGN AND METHODS: The medical histories of 208 hemochromatosis patients were evaluated for eligibility for blood donation from a large prospective database of hemochromatosis patients. A survey that determined the success or exclusion of 81 patients for blood donat...

  3. Whole blood donor deferral analysis at Genera l hospital blood bank – A retrospective study.

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    Hinal Gajjar

    2014-07-01

    Full Text Available Background: A blood transfusion is a life saving procedure in many instances and it requir es an adequate supply of safe blood. The most important is the selection of blood donors by donor selection criteria. A large number of blood donors are deferred from donating blood for several reasons either temporarily or permanently. A shortage of safe blood donor is frequent and it is important to understand the causes of deferral of potential donors. Aims: 1. Primary objective of this study was to record and document the current rate and reasons for donor deferred in our blood bank. 2. Apply relevant findings to modify recruitment strategy for blood donors. Materials and methods: Data for WBD presenting for blood donation in a blood bank V S General Hospital and outdoor camps over two and half years from January 2011 to June 2013 were analysed retrospectively. National Guidelines were used for selection & deferral of WBD. Results: 3836 (11.16% WBD were deferred out of 34373 registered donors during the study period. Donor deferrals were tabulated into temporary and permanent reasons. Temporary deferrals were 3113 (81.15% and permanent deferrals were 723 (18.85%. The most common reasons were low haemoglobin (48.33%, high blood pressure (11.94%, underweight donors (7.95% and history of medication use (6.13% among total deferral donors. Majority of donors (47.16% deferred were between 18 -30 years. Females were found to have higher deferral rate (25.48% than males (10.76%. Discussion and conclusion: A deferral study in blood donors may shed light on the health status of general population which may affect the present and future blood supply. It is important to provide donors with a clear message on their deferral status so that most of temporary deferral donors will return for donation in future.

  4. Subclinical leukopenia in a cross section of Nigerian blood donors

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    Adias TC

    2011-05-01

    Full Text Available Zaccheaus A Jeremiah1, Ruth E Umoh2, Teddy C Adias31Haematology and Blood Transfusion Science Unit, Department of Medical Laboratory Sciences, College of Health Sciences, Niger Delta University, Bayelsa State, Nigeria; 2Department of Haematology and Blood Transfusion, University of Port Harcourt Teaching Hospital, Port Harcourt, Nigeria; 3College of Health Technology, Ogbia, Bayelsa State, NigeriaBackground: Most studies on blood donors are centered on the selective evaluation of red blood cell parameters with little or no regard to white blood cells and platelets.Methods: One hundred and twelve male blood donors comprising 43 first-time and 69 regular blood donors, drawn from a tertiary health facility in the Niger Delta of Nigeria, participated in the study. Their hematological parameters were assessed using a 3-part differential autohematology analyzer (PCE-210 N.Results: Of the blood donors, 18.8% were found to be anemic (packed cell volume [PCV] <33% while 12.5% were leukopenic (white blood cell [WBC] count <2.0 × 109/L. The leukopenia observed in this study was largely due to the significant reductions in both the absolute lymphocyte (P = 0.019, percent lymphocyte (P = 0.016, and percent monocyte count (P = 0.008. Anemia was obviously due to the reduction in the red blood cell (RBC count, PCV, hemoglobin (Hb, mean cell Hb (MCH, and red cell distribution width (RDW values. Significant positive correlations were found between PCV and total WBC count (r = 0.309; P < 0.01 while a negative correlation was found between PCV and absolute lymphocyte counts (r = —0.191; P < 0.005 and RDW (r = —0.219; P < 0.05. Comparison of the mean values of the first-time versus regular blood donor parameters revealed that six parameters were significantly reduced in the regular blood donors (PCV, absolute WBC count, percent monocytes, RBC count, Hb, PCV. The reference ranges of hematological parameters for Port Harcourt donors are also presented in this

  5. THE STUDY OF DEFERRED BLOOD DONORS AT TERTIARY LEVEL HOSPITAL BASED BLOOD BANK OF SOUTH GUJARAT

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    Snehal

    2015-03-01

    Full Text Available BACKGROUND: Pre - donation donor screening is must for the safety of the blood donor and recipient. Deferrals lead to loss of precious whole blood donors and blood units available for transfusion purposes. AIM: T o record and document the current rate and reasons for donor deferral in our tertiary care hospital based blood bank to modify recruitment strategy for blood donors. MATERIAL AND METHODS: Study was conducted by retrospective data analysis of whole blood donors ( V oluntary/ R eplacement , arrived for donating bloo d at blood bank and in outdoor camps , during the period 1 st July 2010 to 30 th June 2014. The donor selection was done by pre - donation screening tests like questionnaire followed by physical examination and haemoglobin estimation. National guidelines were used for selection and deferral of donors. The deferred donor’s data was analyzed statistically. RESULT: Out of 34380 blood donors who came to donate blood, 31049 (89 . 63 %, out of which 97 . 51 % voluntary donors were eligible for d onation and 3331 (10 . 37% blood donors were deferred. The deferral rate among male population 7 . 47% and female population 42 . 09% were observed. Odds ratio for deferral in female donors was 8 . 99, implying thereby that chance of deferral in females is nearly 9 times higher as compared to males. The five leading causes for male donor deferral were low haemoglobin, hypertension, medication, and malaria & alcohol intake in last 48 hours & for the female donor deferral were low haemoglobin, menstruation, medication, low weight & hypotension. CONCLUSIONS: Studying the frequency and the different causes of donor deferral will help to identify sections of the population which could be targeted for increasing and retaining of the existing pool of voluntary blood donors and also to guide and provide the necessary essential database for the policy design and programme implementation at local, regional, and national level.

  6. Evaluation of the return rate of volunteer blood donors

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    Adriana de Fátima Lourençon

    2011-06-01

    Full Text Available BACKGROUND: To convert first-time blood donors into regular volunteer donors is a challenge to transfusion services. OBJECTIVES: This study aims to estimate the return rate of first time donors of the Ribeirão Preto Blood Center and of other blood centers in its coverage region. METHODS: The histories of 115,553 volunteer donors between 1996 and 2005 were analyzed. Statistical analysis was based on a parametric long-term survival model that allows an estimation of the proportion of donors who never return for further donations. RESULTS: Only 40% of individuals return within one year after the first donation and 53% return within two years. It is estimated that 30% never return to donate. Higher return rates were observed among Black donors. No significant difference was found in non-return rates regarding gender, blood type, Rh blood group and blood collection unit. CONCLUSIONS: The low percentage of first-time donors who return for further blood donation reinforces the need for marketing actions and strategies aimed at increasing the return rates.

  7. PSYCHOSOCIAL VARIABLES OF HIGHLY MOTIVATED VOLUNTARY BLOOD DONORS AT BLOOD BANK OF A MEDICAL COLLEGE

    Directory of Open Access Journals (Sweden)

    Bhalodia Jignasa N

    2012-02-01

    Full Text Available Background: The adequacy of blood depends on blood donation rates and numbers of blood donors. To prepare adequate blood supplies, it is essential to investigate the factors that motivate individuals from donating. This study aimed to identify the character of highly motivated donors. This study was undertaken to study the motivational factors leading to voluntary blood donation and understanding the psychosocial variables of blood donors. Methodology: We selected 50 regular blood donors who have donated blood five or more than five times. Results: It was observed that most of the voluntary donors were males (94% and belonged to age group 41-50 years (42%. Most of the donors (86% had studied up to graduate and above. 70% donors have donate up to 30 times and 88% donors have started their first donation before 25 years of age. 94% donors have donated blood first time in voluntary blood donation camp. Conclusion: Motivation, recruitment and retention of voluntary blood donors are important criteria to achieve safe blood donation. [National J of Med Res 2012; 2(1.000: 39-41

  8. Android Blood Donor Life Saving Application in Cloud Computing

    Directory of Open Access Journals (Sweden)

    T.Hilda Jenipha

    2016-07-01

    Full Text Available Emergency situations, such as accidents, create an immediate, critical need for specific blood type. In addition to emergency requirements, advances in medicine have increased the need for blood in many on-going treatments and elective surgeries. Despite increasing requirements for blood, only about 5% of the Indian population donates blood. In this paper we propose a new and efficient way to overcome such scenarios with our project. We have to create a new idea, just touch the button. Donor will be prompted to enter an individual's details, like name, phone number, and blood type. After that your contact details will appear in alphabetical order on the screen; the urgent time of a blood requirement, you can quickly check for contacts matching a particular or related blood group and reach out to them via Phone Call/SMS through the Blood donor App. Blood Donor App provides list of donors in your city/area. Use this app in case of emergency. A large number of blood donors are attracted using an Android application. Cloud- based services can prove important in emergency blood delivery since they can enable central and immediate access to donors' data and location from anywhere. Since almost everyone carries a mobile phone with him, it ensures instant location tracking and communication. The location-based app, operational on android platform, will help users easily find donors of matching blood groups in their location and access their mobile numbers for instant help. Only a registered person, with willingness to donate blood, will be able to access the service.

  9. Knowledge and awareness about Iron deficiency and megaloblastic anaemia among blood donors: a study at rural based tertiary care hospital

    Directory of Open Access Journals (Sweden)

    Amar R. Shah

    2015-06-01

    Full Text Available Background: Voluntary blood donation is promoted in order to make the blood banking safe and successful. Research in the area of blood donation has found that, iron stores are influenced by regular blood donation if dietary intake of iron is inadequate. Awareness and knowledge among blood donors regarding iron and B12 deficiency and its prevention is very much required. Objective: To assess the knowledge and awareness about iron deficiency and megaloblastic anaemia among blood donors. Methods: A cross sectional study was conducted among the 500 voluntary blood donors during the span of 1 year at one of the blood bank of tertiary care hospital using prestructured questionnaire on the various aspect of iron and B12 deficiency/folate deficiency anemia. The data was analysed with the help of Microsoft excel and SPSS. Results: Out of 500 blood donors, 15.6% donors were regular blood donor. It was observed 60% blood donors were having knowledge of anemia in general. Iron deficiency and vitamin B12 deficiency were known to 42% and 31.6% donors, respectively. Only 20% donors could able to answer the acceptable level of hemoglobin require for donating the blood. About 42% donors were aware about importance of iron, folate and vitamin B12 in maintaining normal hemoglobin level. Majority (82.7% of regular blood donors were willing to get information regarding iron, folate and vitamin B12 deficiency. Conclusion: Significant lack of awareness regarding iron and vitamin B12 deficiency was observed in regular voluntary blood donors. The present study recommends the provision of health education on iron and vitamin B12 deficiency as well as Iron, folate and vitamin B12 rich foods to regular blood donor to prevent anaemia among them. [Int J Res Med Sci 2015; 3(3.000: 708-710

  10. Sero prevalence of hepatitis -C antibodies in blood donors

    International Nuclear Information System (INIS)

    Objective: To assess the prevalence of anti HCV antibodies in blood donors. Design: The retrospective sero-epidemiological data of the institute of Hematology and Blood Transfusion Service, Punjab over a period of one year after starting HCV screening, was analyzed to estimate the percentage prevalence. Setting; The data was obtained regularly from the blood units established by this institute at the pablic sector hospitals and retesting on initially reactive serum sample by EIA was done at the Institute. Subjects: A total of 166183 directed first time donors or replacement blood donors aged 18-60 years who donated blood at these blood banks or at mobile sessions have been included in the study. All initially reactive donors who tested non-reactive on EIA were excluded from the study. Main outcome Measures: Assessment of prevalence of HCV in blood donors. Results: 4.45% of the total donors intially tested reactive of these 0.36 % were atsety reactive on intial screening. Further testing by EIA, 4.1%. Conclusions: The blood transfusion service started screening for HCV in April 2000 and the prevalence of HCV, amongst the transfusion transmitted infections (TTIs) being screened for in the Punjab, is the highest. It is almost double the prevalence of HBV and several thousand time that of HIV. Meticulous and total screening coverage is needed to curtail impending catastrophe. With experience, the choice of testing methodology might have to be reviewed. (author)

  11. TRENDS AND PREVALENCE OF INFECTIOUS MARKERS AMONG BLOOD DONORS FROM BLOOD BANK OF A TERTIARY CARE HOSPITAL

    Directory of Open Access Journals (Sweden)

    Sujata Kumbhar

    2015-01-01

    Full Text Available SUMMARY: Blood transfusion services form an integral part of health care; but simultaneously carries the risk of transmission of transfusion transmissible infections. We conducted a 5yr retrospective cross sectional study to estimate the prevalence of various infectious markers in the blood donors which was found to be 2.04%. INTRODUCTION : Blood safety is a major concern among health care personnel. We undertook this study to assess the prevalence of transfusion transmissible infections (TTI's namely - HIV, Hepatitis C, Syphilis and Malaria among blood donors from blood bank of a tertiary care hospital, in Western Maharashtra, India. METHODS : A total of 21,293 blood units were collected from donors. All blood units were screened for HIV, HBs Ag and HCV using ELISA. Test for syphilis was done by Rapid Plasma Reagin card test and peripheral smear examination was done to detect malarial parasite. RESULTS : A total of 21,293 blood donors were tested, of which 19,940 (93.65% were voluntary donors and 1,353 (6.35% were replacement donors. The highest seroprevalence observed was for HBs Ag (1.55% followed by HIV (0.38%, HCV (0.08% and Syphilis (0.02%. No donor was found to be positive for malaria parasite. CONCLUSION : Strategies need to be implemented to improve donor selection, using highly sensitive and specific screening tests and a better structured voluntary donation system. Nucleic acid amplification test would help to detect donors in window period for HIV infection. In view of high prevalence, effective community based programs and health education with emphasis on sexually transmitted diseases may prove helpful to decrease the seroprevalence.

  12. Applicability of available methods for incidence estimation among blood donors

    Institute of Scientific and Technical Information of China (English)

    Shtmian Zou; Edward P.Notari IV; Roger Y.Dodd

    2010-01-01

    @@ Abstract Incidence rates of major transfusion transmissible viral infections have been estimated threugh widely used sereconversion approaches and recently developed methods.A quality database for blood donors and donations with the capacity to track donation history of each donor is the basis for incidence estimation and many other epidemiological studies.Depending on available data,difierent ways have been used to determine incidence rates based on conversion from uninfected to infected status among repeat donors.

  13. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... available when the video has been rented. This feature is not available right now. Please try again ... be donors at http://www.marrow.org . Category Science & Technology License Standard YouTube License Show more Show ...

  14. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... on the use of BMT and PBSCT, see http://www.cancer.gov/cancertopics/fa... If you are ... registry of volunteers willing to be donors at http://www.marrow.org . Category Science & Technology License Standard ...

  15. Seroprevalence of HIV and hepatitis C co-infection among blood donors in Kathmandu Valley, Nepal.

    Science.gov (United States)

    Karki, Surendra; Ghimire, Prakash; Tiwari, Bishnu Raj; Shrestha, Ashish Chandra; Gautam, Avhishekh; Rajkarnikar, Manita

    2009-01-01

    We assessed the seroprevalence of human immunodeficiency virus (HIV) in different categories of blood donors and the hepatitis C virus (HCV) co-infection rate. A total of 33,255 blood samples were screened for HIV using a third generation ELISA test at the Central Blood Transfusion Service, Nepal Red Cross Society, Kathmandu from December 2006 to September 2007. The seroprevalence of HIV was 0.19% (95% CI= 0.15-0.25) and co-infection with HCV was found in 10.8% (95% CI= 4.4-20.9). There were no significant differences in HIV seroprevalence among the different categories of age, sex, type of donation and time of donation. The study revealed a relatively lower seroprevalence of HIV among blood donors in Kathmandu Valley than reported earlier but a higher HCV co-infection rate. The similar seroprevalence between first time and repeat donors suggests the need for more improved donor education and counselling. PMID:19323036

  16. Prevalence of peptic ulcer in Helicobacter pylori positive blood donors.

    OpenAIRE

    Vaira, D; Miglioli, M; Mulè, P; Holton, J; M. Menegatti; Vergura, M; Biasco, G.; Conte, R.; Logan, R P; Barbara, L

    1994-01-01

    This study aimed to determine the importance of raised antibodies to Helicobacter pylori in an asymptomatic population. A total of 128 asymptomatic blood donors who were seropositive for H pylori and consented to endoscopy were investigated. These subjects were from a population of 1010 blood donors screened for antibodies to H pylori. A questionnaire was completed to determine if any subjects had complained of symptoms, and they subsequently had endoscopy. Altogether 121 of 128 were positive...

  17. Tissue banking: relationship with blood donor and organ donor card status.

    Science.gov (United States)

    McKenzie, Kenneth D; Fitzpatrick, Patricia E; Sheehan, John D

    2012-01-01

    Understanding the relationships among altruistic health acts may serve to aid therapeutic research advances. In this paper, we report on the links between two such behaviours-donating blood and carrying an organ donor card-and willingness to donate urological tissue to a tissue bank. Reasons for the differential willingness to do so are examined in this paper. A systematic sample of 259 new and returning attendees at a tertiary urology referral clinic in Ireland completed a self-report questionnaire in an outpatient setting. In addition to demographic details, details of known diagnosis of malignancy and family history of cancer; attitudes to tissue donation for research purposes were gauged using a 5-point Likert scale. Both blood donors and organ donor card carriers were more likely to be willing to donate tissue for research purposes. Blood donors were more likely want to know their overall results in comparison to nonblood donors and want their samples to be used for nonprofit research. Our hypothesis that being a blood donor would be a better predictor to donate urological tissue than being an organ donor card carrier borne out by the trends reported above. PMID:22567418

  18. Lipid profile of regular blood donors

    OpenAIRE

    Uche EI; Adediran A; Damulak OD; Adeyemo TA; Akinbami AA; Akanmu AS

    2013-01-01

    EI Uche,1 A Adediran,2 OD Damulak,3 TA Adeyemo,2 AA Akinbami,4 AS Akanmu21Department of Hematology and Blood Transfusion, Lagos University Teaching Hospital, Lagos, Nigeria; 2Department of Hematology and Blood Transfusion, University of Lagos, Lagos, Nigeria; 3Department of Hematology and Blood Transfusion, Jos University Teaching Hospital, Jos, Nigeria; 4Department of Hematology and Blood Transfusion, Lagos State University, Ikeja, NigeriaIntroduction: A few reports have linked regular blood...

  19. Retention of blood donors: strategies to fulfill the requirements of blood centers

    OpenAIRE

    Adriana de Fátima Lourençon; Rodrigo Guimarães dos Santos Almeida; Oranice Ferreira; Edson Zangiacomi Martinez

    2011-01-01

    BACKGROUND: To convert first-time blood donors into regular volunteer donors is a challenge to transfusion services. OBJECTIVES: This study aims to estimate the return rate of first time donors of the Ribeirão Preto Blood Center and of other blood centers in its coverage region. METHODS: The histories of 115,553 volunteer donors between 1996 and 2005 were analyzed. Statistical analysis was based on a parametric long-term survival model that allows an estimation of the proportion of donors who...

  20. Motivating Factors and Potential Deterrents to Blood Donation in High School Aged Blood Donors

    OpenAIRE

    Rachel Finck; Alyssa Ziman; Matthew Hoffman; Michelle Phan-Tang; Shan Yuan

    2016-01-01

    Background. To ensure an adequate supply of blood, collection centers must design campaigns that successfully recruit and maintain an active donor pool. Understanding factors that motivate and deter individuals from donating may help centers develop targeted recruitment campaigns. These factors among high school aged blood donors have not yet been fully investigated. Study Design and Methods. A voluntary, anonymous survey was administered to student donors at high school mobile blood drives. ...

  1. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... the use of BMT and PBSCT, see http://www.cancer.gov/cancertopics/fa... If you are interested ... of volunteers willing to be donors at http://www.marrow.org . Category Science & Technology License Standard YouTube ...

  2. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... be donors at http://www.marrow.org . Category Science & Technology License Standard YouTube License Show more Show ... views 4:25 Susan Solomon: The promise of research with stem cells - Duration: 14:59. TED 55, ...

  3. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... cell donation experience at the National Institutes of Health Clinical Center in Bethesda, MD. Bone marrow transplantation ( ... About Cord Blood Banking - Duration: 49:19. Children's Health 27,845 views 49:19 Scott: Donating Blood ...

  4. Blood donors on teratogenic drugs and donor deferral periods in a clinical situation.

    Science.gov (United States)

    Shin, S Y; Shin, Y H; Lee, S W; Shin, J Y; Kim, C H

    2012-05-01

    Deferral of blood donors taking teratogenic drugs is critical. From March 2008 to January 2009, we analysed stored blood specimens from donors who had taken teratogenic drugs and whose blood was transfused to women of childbearing age to determine the plasma concentration at the time of donation using high-performance liquid chromatography. In total, 167 specimens were examined. The numbers of specimens exceeding the quantification limit were 7, 39, 4, 2 and 1 for finasteride, isotretinoin, acitretin, etretinate and dutasteride, respectively. Finasteride was beyond the recommended drug deferral period in one specimen. These results may help create practical deferral policies. PMID:22211799

  5. Analysis on effect of health education about toxoplasmosis among blood donors in Zhaoxian County of Hebei Province%河北省赵县献血者弓形虫病健康教育效果

    Institute of Scientific and Technical Information of China (English)

    宋任浩; 何路军; 常缨

    2013-01-01

    [Objective] To evaluate the effect of health education about toxoplasmosis among blood donors, provide the basis for making effective health education strategy. [Methods]By using stratified random cluster sampling, the blood donors received various forms of health education about toxoplasmosis, and the awareness rate and the scores of prevention knowledge before and after education were compared. [Results] After the intervention, the awareness rate and the scores of the knowledge about toxoplasmosis prevention had improved in different degrees, and the qualified rate increased from 8. 14% (25/307) before intervention to 82. 08% (252/307) after intervention. [Conclusion]The omnidirectional and multi-form health education is the effective method to improve the prevention awareness of toxoplasmosis. It is necessary to popularize the knowledge about toxoplasmosis prevention, enhance the self prevention ability, and change the unhealthy diet habit. Meanwhile, serological screening of Toxoplasma Gondii in high risk population of blood donors is the effective method to improve the blood quality and ensure the safe use of blood.%目的 评价对献血者实施弓形虫病健康教育的效果,为制定有效的健康教育策略提供依据.方法 采用分层随机整群抽样方法选择调查对象,在献血者中开展多种形式的弓形虫病健康教育干预,比较干预前后预防知识知晓和得分情况.结果 干预后弓形虫病预防知识知晓率和得分均有不同程度的提高,合格率从干预前的8.14% (25/307)提高到干预后的82.08%(252/307).结论 全方位、多形式的弓形虫病健康教育是提高弓形虫病预防意识的有效方法.在献血者中普及弓形虫病预防知识,提高自我防病能力,改变不良生活饮食习惯,同时应在特殊高危献血人群中开展弓形虫血清学筛查是提高血液质量、保证用血者安全的有效措施.

  6. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... Institutes of Health Clinical Center in Bethesda, MD. Bone marrow transplantation (BMT) and peripheral blood stem cell transplantation (PBSCT) ... Medicine Clinics 225,676 views 6:18 Alicia's bone marrow donation - Duration: 8:33. ... Peripheral Blood Stem Cell Transplant - Duration: 15:50. Dartmouth-Hitchcock 2,764 views ...

  7. Partial phenotyping in voluntary blood donors of Gujarat State

    Directory of Open Access Journals (Sweden)

    Maitrey Gajjar

    2016-01-01

    Full Text Available Introduction: Partial phenotyping of voluntary blood donors has vital role in transfusion practice, population genetic study and in resolving legal issues.The Rh blood group is one of the most complex and highly immunogenic blood group known in humans. The Kell system, discovered in 1946, is the third most potent system at triggering hemolytic transfusion reactions and consists of 25 highly immunogenic antigens. Knowledge of Rh & Kell phenotypes in given population is relevant for better planning and management of blood bank; the main goal is to find compatible blood for patients needing multiple blood transfusions. The aim of this study was to evaluate the frequency of Rh & Kell phenotype of voluntary donors in Gujarat state. Materials and Methods: The present study was conducted by taking 5670 samples from random voluntary blood donors coming in blood donation camp. Written consent was taken for donor phenotyping. The antigen typing of donors was performed by Qwalys-3(manufacturer: Diagast by using electromagnetic technology on Duolys plates. Results: Out of 5670 donors, the most common Rh antigen observed in the study population was e (99.07% followed by D (95.40%, C (88.77%, c (55.89% and E (17.88%. The frequency of the Kell antigen (K was 1.78 %. Discussion: The antigen frequencies among blood donors from Gujarat were compared with those published for other Indian populations. The frequency of D antigen in our study (95.4% and north Indian donors (93.6 was significantly higher than in the Caucasians (85% and lower than in the Chinese (99%. The frequencies of C, c and E antigens were dissimilar to other ethnic groups while the ′e′ antigen was present in high frequency in our study as also in the other ethnic groups. Kell antigen (K was found in only 101 (1.78 % donors out of 5670. Frequency of Kell antigen in Caucasian and Black populations is 9% & 2% respectively. The most common Kell phenotype was K-k+, not just in Indians (96.5% but

  8. [Marrow donor registration and cord blood banking: current issues].

    Science.gov (United States)

    Takanashi, Minoko

    2016-03-01

    Marrow donor registration and cord blood banking are essential components of the infrastructure required for unrelated haemopoietic stem cell transplantations. We now have a new law to support and regulate the Marrow Donor Coordination Agency, Cord Blood Banks and the Haematopoietic Stem Cell Provision Support Organization. We also need to have a specific goal for bone marrow and peripheral blood stem cell donor registration, a minimum cord blood bank size, and the demographic data to back the medical needs for unrelated haemopoietic stem cell transplantations. To improve bone marrow and peripheral blood stem cell transplantations, we need to recruit younger adults for marrow registration and make greater efforts to shorten the coordinating period. For cord blood transplantations, uniting and empowering the cord blood collection sites is needed, to encourage and motivate obstetricians and other staff, as the quality of cord blood units is primarily determined during collection. Also, the cord blood banks must work cooperatively to provide cord blood internationally, which includes coordinating with international agencies and their regulations.

  9. Knowledge, attitude, and beliefs of young, college student blood donors about Human immunodeficiency virus

    Directory of Open Access Journals (Sweden)

    Anju Dubey

    2014-01-01

    Full Text Available Introduction: Young people, who tend to be healthy, idealistic, and motivated, are an excellent pool of potential voluntary unpaid blood donors. Recruiting and retaining young blood donors improves the long term safety and sufficiency of a country′s blood supply. Knowledge, attitude, and beliefs about Human immunodeficiency virus (HIV should play an important role in prevention of disease transmission. Materials and Methods: This study was a questionnaire based survey, conducted to explore the levels of knowledge, attitude, and beliefs about HIV in young college student blood donors. Results: The results showed that the proportion of participants with comprehensive knowledge of HIV prevention and transmission was lesser than expected. Increase in education level and male gender was found to be significantly associated with high HIV-related knowledge. The responses on the different aspects of HIV-related attitude were also varied and there is still stigma associated with Acquired Immunodeficiency Syndrome (AIDS even in the educated groups. Discussion: There was a spectrum of myths and misperceptions emphasizing the need of education that recognizes the social context of attitude towards HIV. Results from this study may contribute to the development of appropriate educational and training material for this group of donors which in turn, may assist in achieving the elusive goal of safe blood supply in future.

  10. Partial phenotyping in voluntary blood donors of Gujarat State

    OpenAIRE

    Maitrey Gajjar; Tarak Patel; Nidhi Bhatnagar; Kruti Patel; Mamta Shah; Amit Prajapati

    2016-01-01

    Introduction: Partial phenotyping of voluntary blood donors has vital role in transfusion practice, population genetic study and in resolving legal issues. The Rh blood group is one of the most complex and highly immunogenic blood group known in humans. The Kell system, discovered in 1946, is the third most potent system at triggering hemolytic transfusion reactions and consists of 25 highly immunogenic antigens. Knowledge of Rh & Kell phenotypes in given population is relevant for better pla...

  11. ASSESSMENT OF SEROPREVALANCE OF HUMAN IMMUNODEFICIENCY VIRUS INFECTION AMONG BLOOD DONORS IN AND AROUND BELLARY, KARNATAKA STATE, INDIA

    Directory of Open Access Journals (Sweden)

    Huggi

    2014-11-01

    Full Text Available AIMS AND OBJECTIVES: The aim of the study was to analyse the seroprevalence of human immunodeficiency virus (HIV infection in the blood among healthy voluntary blood donors in and around Bellary. SAMPLE SIZE: 51,144 blood donors. STUDY DESIGN: Retrospective study. DURATION OF THE STUDY: Jan-2006 to Dec-2013. RESULTS: In the 8-year study period, 51,144 units of blood were collected. The Seroprevalence of HIV was found to be 0.38%. Also, the Seroprevalence of HIV in Voluntary Blood Donors and Replacement Blood Donors was found to be 0.35% and 0.81%. In males and female blood donors, the Seroprevalence was fond to be 0.38% and 0.39%. CONCLUSION: The 8 year study reveals that the Seroprevalence of HIV in replacement donors is nearly twice as that of voluntary donors and nearly equal in male and female donors. Screening the blood donors for IV infection has to be made mandatory and the tests should be of the highest quality. Education and awareness among people should be encouraged and imparted.

  12. ORIGINAL ARTICLE: Blood Donor’s Status of HIV, HBV, HCV and Syphilis in this Region of Marathwada, India

    Directory of Open Access Journals (Sweden)

    Rangrao H. Deshpande

    2012-07-01

    Full Text Available Aims & Objectives: Blood transfusion can cause the transmission of infections to recipients. This is an important mode of infection. The aim of study was to assess the prevalence of such type of infections among blood donors and to compare the seroprevalence of transfusion transmitted diseases in voluntary donors and replacement donors. Retrospective study of five years from Jan. 2007 to Dec. 2011 was done. This study was conducted at Blood bank, MIMSR Medical College Latur, Govt. Medical College, Latur and Bhalchandra Blood bank, Latur. Material & Methods: Total 10, 4925 donors were tested. Donors were screened for seroprevalence of HIV, HBC, HCV and Syphilis. Screening of HIV, HBV & HCV was done by ELISA method & Syphilis was screened by RPR type. Results: The comparison of seroprevalence of HIV, HBV, HCV & Syphilis in voluntary donors and replacement donors showed significant difference only for HIV in the years 2007, 2010, and 2011. Conclusion: The seroprevalence of transfusion transmitted diseases in the study is very low or negligible in voluntary donors as compared to replacement donors. There was a declining trend of seroprevalence for all the disease screened. But in our study the difference is not significant, which indicates that the selection of donors is of low quality. The selection of high quality voluntary donors should be achieved by creation of awareness by education of the prospective donor populations.

  13. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... Institutes of Health Clinical Center in Bethesda, MD. Bone marrow transplantation (BMT) and peripheral blood stem cell transplantation (PBSCT) are most commonly used in the treatment of cancers like leukemia and lymphoma to restore stem cells ...

  14. Declining prevalence of hepatitis E antibodies among Danish blood donors

    DEFF Research Database (Denmark)

    Holm, Dorte K; Moessner, Belinda K; Engle, Ronald E;

    2015-01-01

    BACKGROUND: The increasing incidence of reported hepatitis E cases in Europe has focused attention on hepatitis E virus (HEV) and the risk of transfusion-transmitted hepatitis E. The aim of this study was to investigate the prevalence of antibodies to HEV (anti-HEV) among Danish blood donors in...... 2013 and to compare it to previous studies in Denmark. In addition we wanted to compare the relative reactivity of two different assays. STUDY DESIGN AND METHODS: Samples from 504 blood donors were collected and analyzed for anti-HEV with an in-house assay developed at the National Institutes of Health...... (NIH). In addition the samples were analyzed with the Wantai anti-HEV assay. Demographic information and possible HEV exposure was collected by self-administered questionnaire. RESULTS: Using the NIH assay the prevalence of anti-HEV among Danish blood donors was 10.7% and with the Wantai assay the...

  15. Donor exclusion in the National Blood Service Tissue Services living bone donor programme.

    Science.gov (United States)

    Pink, F; Warwick, R M; Purkis, J; Pearson, J

    2006-01-01

    National Blood Service (NBS) Tissue Services (TS) operates living donor and deceased donor tissue banking programmes. The living bone donor programme operates in collaboration with 91 orthopaedic departments across the country and collects bone donations, in the form of surgically removed femoral heads (FHs), from over 5,000 patients per annum undergoing total hip replacement. Bone donated via the living programme constitutes approximately 55% of the total bone donated to NBS. Non-NBS tissue banks, primarily in hospital orthopaedic departments, also bank donated bone for the UK. A survey of information received from 16 collaborating orthopaedic centres, between April 2003 and August 2004, identified 709 excluded donors. The total number of donations banked from these sites was 1,538. Donations can be excluded before collection if there are contraindications noted in a potential donor's medical history before their operation. Donors may also be excluded after collection of the FH, for instance because of reactive microbiology tests for blood borne viruses, or if the donation storage conditions or related documentation have not met stringent quality requirements. In this survey, bone or joint conditions were the major reasons for excluding potential donors before donation (154 of 709 exclusions, 22%), followed by a current or a past history of malignancy (139 of 709 exclusions, 20%). Local staffing and operational difficulties sometimes resulted in potential donors being missed, or specific reasons for exclusion not being reported (117 exclusions). These out numbered exclusions due to patient refusal (80 exclusions). A small number (quality. Training to ensure that standards are complied with and a firm evidence base for exclusion criteria, applied uniformly, will help focus donor identification efforts on individuals meeting rational criteria so that fewer potential donations are lost. PMID:16511660

  16. Donor exclusion in the National Blood Service Tissue Services living bone donor programme.

    Science.gov (United States)

    Pink, F; Warwick, R M; Purkis, J; Pearson, J

    2006-01-01

    National Blood Service (NBS) Tissue Services (TS) operates living donor and deceased donor tissue banking programmes. The living bone donor programme operates in collaboration with 91 orthopaedic departments across the country and collects bone donations, in the form of surgically removed femoral heads (FHs), from over 5,000 patients per annum undergoing total hip replacement. Bone donated via the living programme constitutes approximately 55% of the total bone donated to NBS. Non-NBS tissue banks, primarily in hospital orthopaedic departments, also bank donated bone for the UK. A survey of information received from 16 collaborating orthopaedic centres, between April 2003 and August 2004, identified 709 excluded donors. The total number of donations banked from these sites was 1,538. Donations can be excluded before collection if there are contraindications noted in a potential donor's medical history before their operation. Donors may also be excluded after collection of the FH, for instance because of reactive microbiology tests for blood borne viruses, or if the donation storage conditions or related documentation have not met stringent quality requirements. In this survey, bone or joint conditions were the major reasons for excluding potential donors before donation (154 of 709 exclusions, 22%), followed by a current or a past history of malignancy (139 of 709 exclusions, 20%). Local staffing and operational difficulties sometimes resulted in potential donors being missed, or specific reasons for exclusion not being reported (117 exclusions). These out numbered exclusions due to patient refusal (80 exclusions). A small number (quality. Training to ensure that standards are complied with and a firm evidence base for exclusion criteria, applied uniformly, will help focus donor identification efforts on individuals meeting rational criteria so that fewer potential donations are lost.

  17. Thalassemia and Hemoglobin E in Southern Thai Blood Donors

    Directory of Open Access Journals (Sweden)

    Manit Nuinoon

    2014-01-01

    Full Text Available Thalassemia and hemoglobin E (Hb E are common in Thailand. Individuals with thalassemia trait usually have a normal hemoglobin concentration or mild anemia. Therefore, thalassemic individuals who have minimum acceptable Hb level may be accepted as blood donors. This study was aimed at determining the frequency of α-thalassemia 1 trait, β-thalassemia trait, and Hb E-related syndromes in Southern Thai blood donors. One hundred and sixteen voluntary blood donors, Southern Thailand origin, were recruited for thalassemia and Hb E screening by red blood cell indices/dichlorophenolindophenol precipitation test. β-Thalassemia and Hb E were then identified by high performance liquid chromatography and 4 common α-thalassemia deletions were characterized by a single tube-multiplex gap-polymerase chain reaction. Overall frequency of hemoglobinopathies was 12.9%, classified as follows: homozygous α-thalassemia 2 (1.7%, heterozygous α-thalassemia 1 (1.7%, heterozygous β-thalassemia without α-thalassemia (0.9%, heterozygous Hb E without α-thalassemia (5.2%, double heterozygotes for Hb E/α-thalassemia 1 (1.7%, homozygous Hb E without α-thalassemia (0.9%, and homozygous Hb E with heterozygous α-thalassemia 2 (0.9%. The usefulness of thalassemia screening is not only for receiving highly effective red blood cells in the recipients but also for encouraging the control and prevention program of thalassemia in blood donors.

  18. Ethical Aspects of Blood Donors and the Recipients of Their Blood

    Directory of Open Access Journals (Sweden)

    P. J. M. van den Burg

    2012-01-01

    Full Text Available To date medical care is inextricable based on blood donors and blood products. The continuing increase and intensification of tests and guidelines also results in a change in deferral and abnormal test results. Donors and recipients of their blood are faced with this information and are confronted with a kaleidoscope of thoughts and emotions. The discussion with respect to paid versus nonpaid donation is not new, but other aspects are less often discussed. We describe these other aspects for donors and recipients of their blood and hope to open the ethical discussion; if and to what extent we should have limits?

  19. Prevalence of hepatitis B virus infection among blood donors at the Tamale Teaching Hospital, Ghana (2009

    Directory of Open Access Journals (Sweden)

    Dongdem Julius

    2012-02-01

    Full Text Available Abstract Background Despite education and availability of drugs and vaccines, hepatitis B virus (HBV is still the most common severe liver infection in the world accounting for >1 million annual deaths worldwide. Transfusion of infected blood, unprotected sex and mother to child transmission are 3 key transmission routes of HBV in Ghana. There is high incidence of blood demanding health situations in northern Ghana resulting from anemia, accidents, malnutrition, etc. The higher the demand, the higher the possibility of transmitting HBV through infected blood. The aim of the investigation was to estimate the prevalence of HBV in blood donors which will provide justification for interventions that will help minimize or eliminate HBV infection in Ghana. Findings We investigated the prevalence of HBV infection among blood donors at Tamale Teaching Hospital. The Wondfo HBsAg test kit was used to determine the concentration of HBsAg in 6,462 (576 voluntary and 5,878 replacement donors as being ≥1 ng/ml. 10.79% of voluntary donors and 11.59% of replacement donors were HBsAg+. The 20-29 year group of voluntary donors was >2 times more likely to be HBsAg + than 40-60. Also the 20-29 year category of replacement donors was >4 times as likely to be HBsAg + than 50-69. Conclusions Risk of infection was age, sex and donor type dependent. The 20-29 year category had the highest prevalence of HBsAg + cases, mostly males residing within the metropolis.

  20. Human T-cell lymphotropic virus in volunteer blood donors.

    Science.gov (United States)

    Taylor, P E; Stevens, C E; Pindyck, J; Schrode, J; Steaffens, J W; Lee, H

    1990-01-01

    Serum samples collected in 1985 and 1986 from 18,257 donors to the Greater New York Blood Program were screened by enzyme-linked immunoassay for antibody to human T-cell lymphotropic virus (anti-HTLV). Fifteen samples (0.08%) were confirmed positive: 7 by radioimmunoprecipitation assay (RIPA) alone, 6 by Western blot alone, and 2 by combined results from both tests. One donor, whose original test result was uninterpretable because multiple nonspecific bands were present on RIPA, clearly tested positive on subsequent specimens. Follow-up testing of individuals with this type of result may be needed to resolve their HTLV status. Anti-HTLV prevalence increased with age and was significantly more common in black or Hispanic donors and in those born in the Caribbean than in other donors. All anti-HTLV-positive donors were negative for antibody to HIV-1, and only one donor (7% of those positive) would have been excluded by any of the routine donor screening tests used at that time. PMID:2173176

  1. Laboratory and Genetic Assessment of Iron Deficiency in Blood Donors

    OpenAIRE

    Kiss, Joseph E.

    2014-01-01

    Over 9 million individuals donate blood annually in the US. Between 200 to 250 mg of iron is removed with each whole blood donation, reflecting losses from the hemoglobin in red blood cells. This amount represents approximately 25% of the average iron stores in men and almost 75% of the iron stores in women. Replenishment of iron stores takes many months, leading to a high rate of iron depletion, especially in frequent blood donors (e. g., more than 2 times per year). In large epidemiologic s...

  2. Adverse Reactions in Allogeneic Blood Donors: A Tertiary Care Experience from a Developing Country

    OpenAIRE

    Sadia Sultan; Mohammad Amjad Baig; Syed Mohammed Irfan; Syed Ijlal Ahmed; Syeda Faiza Hasan

    2016-01-01

    Objectives: Fragmented blood transfusion services along with an unmotivated blood donation culture often leads to blood shortage. Donor retention is crucial to meet the increasing blood demand, and adverse donor reactions have a negative impact on donor return. The aim of this study was to estimate adverse donor reactions and identify any demographic association.   Methods: We conducted a prospective study between January 2011 and December 2013. A total of 41,759 healthy donors were enrol...

  3. Molecular genotyping of HCV infection in seropositive blood donor

    Science.gov (United States)

    Zarin, Siti Noraziah Abu; Ibrahim, Nazlina

    2013-11-01

    This study is to investigate the prevalence of hepatitis C virus infection in seropositive blood donor. RNA was extracted from 32 positive samples in National Blood Centre and Melaka Hospital. The core and NS5B sequences were obtained from 23 samples. Genotype 3a is most prevalent in this study followed by genotype 1a. Evidence of mixed-genotypes (3a and 1b) infections was found in 5 subjects.

  4. High Rates of Hepatitis B and C and HIV Infections among Blood Donors in Cameroon: A Proposed Blood Screening Algorithm for Blood Donors in Resource-Limited Settings

    Directory of Open Access Journals (Sweden)

    Florent Fouelifack Ymele

    2012-01-01

    , 4.44%  (n=206, and 1.44%  (n=67, respectively. Coinfection with HIV and HBV was observed among 0.77% donors, followed by hepatitis B and C co-infection (0.21% and HIV and HCV coinfection (0.06%. Co-infection with HIV-HBV-HCV was encountered in 2 donors. The HIV, HBV, and HCV infections lead to a destruction of one out of six sets of blood collected. Conclusion. There is a need to review policies for blood collection from donors, by modifying the algorithm of blood donors testing. Pretesting potential donors using rapid tests could help to avoid collection and destruction of (infected blood.

  5. Henan Surveys Blood Donors to Aid AIDS Prevention

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    Central China's Henan Province, one of the worst AIDS-hit provinces in the country, launched a sweeping survey in September of past blood donors to ascertain tile number of HIV-infected individuals to aid AIDS prevention and control work.

  6. The prevalence of transfusion transmitted virus infection in blood donors

    Institute of Scientific and Technical Information of China (English)

    Cheng Hui Huang; Ru Guang Chen; Yu Sen Zhou; Hai Tao Wang; Chun Ying Xie

    2000-01-01

    @@INTRODUCTION A newly discovered DNA virus,transfusion transmitted virus (TTV), was reported as a cause of post-transfusion hepatitis of unknown etiology in Japan[1]. In order to investigate TTV prevalence in southern China, a study was carried out among blood donors, patients with liver diseases and hemodialysis to determine the epidemiological charateristics.

  7. Association of ABO and Rh Blood Groups to Blood-Borne Infections among Blood Donors in Tehran-Iran.

    OpenAIRE

    Fatemeh Mohammadali; Aliakbar Pourfathollah

    2014-01-01

    Abstract Background The aim of this study was to investigate the prevalence of hepatitis B, hepatitis C, HIV and syphilis infections in blood donors referred to Tehran Blood Transfusion Center (TBTC), and determine any association between blood groups and blood- borne infections between the years of 2005 and 2011. Methods This was a retrospective study conducted at TBTC. All of the donor serum samples were screened for HBV, HCV, HIV and syphilis by using third generation ELISA kits and RPR te...

  8. STUDY ON KNOWLEDGE AND ATTITUDE OF BLOOD DONORS TOWARDS BLOOD DONATION IN BIJAPUR DISTRICT

    OpenAIRE

    Himanshu Dilip; Prakash M

    2016-01-01

    BACKGROUND Blood is the living force of our body and there is no substitute for it. It can only be replaced through blood donation. Blood donation can save millions of lives. Demand for safe blood is increasing every day because of increase in population, increased life-expectancy and urbanisation, trauma cases, major surgeries, patients with regular transfusion requirement like cases of thalassaemia, haemophilia and chemotherapy. To increase blood donor recruitment and retenti...

  9. HFE gene mutations and iron status of Brazilian blood donors

    Directory of Open Access Journals (Sweden)

    P.C.J.L. Santos

    2010-01-01

    Full Text Available Mutations of the HFE and TFR2 genes have been associated with iron overload. HFE and TFR2 mutations were assessed in blood donors, and the relationship with iron status was evaluated. Subjects (N = 542 were recruited at the Hemocentro da Santa Casa de São Paulo, São Paulo, Brazil. Iron status was not influenced by HFE mutations in women and was independent of blood donation frequency. In contrast, men carrying the HFE 282CY genotype had lower total iron-binding capacity (TIBC than HFE 282CC genotype carriers. Men who donated blood for the first time and were carriers of the HFE 282CY genotype had higher transferrin saturation values and lower TIBC concentrations than those with the homozygous wild genotype for the HFE C282Y mutation. Moreover, in this group of blood donors, carriers of HFE 63DD plus 63HD genotypes had higher serum ferritin values than those with the homozygous wild genotype for HFE H63D mutation. Multiple linear regression analysis showed that HFE 282CY leads to a 17.21% increase (P = 0.018 and a 83.65% decrease (P = 0.007 in transferrin saturation and TIBC, respectively. In addition, serum ferritin is influenced by age (3.91%, P = 0.001 and the HFE 63HD plus DD genotype (55.84%, P = 0.021. In conclusion, the HFE 282Y and 65C alleles were rare, while the HFE 63D allele was frequent in Brazilian blood donors. The HFE C282Y and H63D mutations were associated with alterations in iron status in blood donors in a gender-dependent manner.

  10. Tissue Banking: Relationship with Blood Donor and Organ Donor Card Status

    OpenAIRE

    McKenzie, Kenneth D.; Fitzpatrick, Patricia E.; Sheehan, John D.

    2012-01-01

    Understanding the relationships among altruistic health acts may serve to aid therapeutic research advances. In this paper, we report on the links between two such behaviours—donating blood and carrying an organ donor card—and willingness to donate urological tissue to a tissue bank. Reasons for the differential willingness to do so are examined in this paper. A systematic sample of 259 new and returning attendees at a tertiary urology referral clinic in Ireland completed a self-report questi...

  11. Blood donors' motivation and attitude to non-remunerated blood donation in Lithuania

    Directory of Open Access Journals (Sweden)

    Blazeviciene Aurelija

    2006-06-01

    Full Text Available Abstract Background In the Soviet period, the blood donation system operated in Lithuania exclusively on a remunerative basis. After joining the EU, Lithuania committed itself to meeting the EU requirements to provide all consumers within its boundaries with safe blood products made from voluntary unpaid blood donations. However, the introduction of a non-remunerated donation system may considerably affect donors' motivation and retention. Thus the aim of the current research was to determine blood donation motives among the present donors and investigate their attitude towards non-remunerated donation. Methods A questionnaire survey of 400 blood donors. Survey data processed using SPSS statistical analysis package. Statistical data reliability checked using Fisher's exact test (p Results Paid donors comprised 89.9%, while non-paid ones made 10.1% of the respondents. Research findings show that 93 per cent of the paid donors give blood on a regular basis; while among the non-remunerated donors the same figure amounted merely to 20.6 per cent. The idea of the remuneration necessity is supported by 78.3 per cent of the paid donors, while 64.7 per cent of the non-remunerated respondents believe that remuneration is not necessary. The absolute majority of the paid donors (92% think they should be offered a monetary compensation for blood donation, while more than half of the non-remunerated donors (55.9 claim they would be content with a mere appreciation of the act. Provided no remuneration were offered, 28.44 per cent of the respondents would carry on doing it, 29.6 per cent would do it only in emergency, 29.6 per cent would donate blood merely for their family or friends, and 12.3 per cent would quit it completely. Conclusion Most respondents admitted having donated blood for the following reasons: willingness to help the ill or monetary compensation. Majority would consent to free blood donation only in case of emergency or as a family

  12. Motivating Factors and Potential Deterrents to Blood Donation in High School Aged Blood Donors.

    Science.gov (United States)

    Finck, Rachel; Ziman, Alyssa; Hoffman, Matthew; Phan-Tang, Michelle; Yuan, Shan

    2016-01-01

    Background. To ensure an adequate supply of blood, collection centers must design campaigns that successfully recruit and maintain an active donor pool. Understanding factors that motivate and deter individuals from donating may help centers develop targeted recruitment campaigns. These factors among high school aged blood donors have not yet been fully investigated. Study Design and Methods. A voluntary, anonymous survey was administered to student donors at high school mobile blood drives. The survey instrument asked the students to rate several potential motivating factors in their importance in the decision to donate blood and several potential deterring factors in their future decision whether or not to donate blood again. The survey also asked the students to rate the desirability of several potential incentives. Results. Motivating factors that reflected prosocial, empathetic, and altruistic thoughts and beliefs were rated highly by students. Pain from phlebotomy was most commonly chosen as potential deterrent. Movie tickets and cookies/snacks at the drive were rated as the most attractive incentives. Conclusion. High school aged blood donors are similar to other donor groups in their expressed motives for donating blood. This group may be unique in the factors that deter them from donating and in their preferences for different incentives. PMID:27293985

  13. Motivating Factors and Potential Deterrents to Blood Donation in High School Aged Blood Donors

    Directory of Open Access Journals (Sweden)

    Rachel Finck

    2016-01-01

    Full Text Available Background. To ensure an adequate supply of blood, collection centers must design campaigns that successfully recruit and maintain an active donor pool. Understanding factors that motivate and deter individuals from donating may help centers develop targeted recruitment campaigns. These factors among high school aged blood donors have not yet been fully investigated. Study Design and Methods. A voluntary, anonymous survey was administered to student donors at high school mobile blood drives. The survey instrument asked the students to rate several potential motivating factors in their importance in the decision to donate blood and several potential deterring factors in their future decision whether or not to donate blood again. The survey also asked the students to rate the desirability of several potential incentives. Results. Motivating factors that reflected prosocial, empathetic, and altruistic thoughts and beliefs were rated highly by students. Pain from phlebotomy was most commonly chosen as potential deterrent. Movie tickets and cookies/snacks at the drive were rated as the most attractive incentives. Conclusion. High school aged blood donors are similar to other donor groups in their expressed motives for donating blood. This group may be unique in the factors that deter them from donating and in their preferences for different incentives.

  14. HIV prevalence among blood donors in a blood bank in Curitiba (Brazil

    Directory of Open Access Journals (Sweden)

    Andrade Neto José Luiz

    2002-01-01

    Full Text Available There still is no cure for the acquired immunodeficiency syndrome (AIDS. Its etiologic agent is the human immunodeficiency virus (HIV, and transmission occurs through sexual relationships, contacts with blood, and vertically (mother to child. In this study, we sought to determine the prevalence of HIV among blood donors at a blood bank in Curitiba. We studied 213,666 blood donations made from March 1, 1992, to April 25, 1999. Each potential blood donor first answered a questionnaire, submitted to a clinical examination, and filled out a self-exclusion card. Blood was collected and analyzed only from the candidates approved by the first two criteria. Two tests were used to detect HIV: ELISA for screening, and Western-Blot for confirmation. The results were analyzed statistically by determining the 95% confidence interval. Of the total number of donors, 156,942 were men, and 56,724 were women. There were 319 cases of HIV infection (244 men, 75 women. There were no significant differences between genders, or among the different age groups, or between first-donation and repeated-donation donors. There was a significant predominance of HIV infection among single individuals compared to married, widowed, and other individuals. The same occurred among married and divorced individuals compared to widowed subjects. The prevalence of HIV among blood donors was 0.149% (0.155% among men and 0.132% among women. The frequency of HIV was statistically identical among new blood donors and repeat donors. A large number of HIV-infected married women was also observed.

  15. Digital questionnaire platform in the Danish Blood Donor Study

    DEFF Research Database (Denmark)

    Burgdorf, K S; Felsted, N; Mikkelsen, S;

    2016-01-01

    their personal civil registration numbers. The questionnaire is placed at a front-end web server and a collection server retrieves the completed questionnaires. Data from blood samples, register data, genetic data and verification of signed informed consent are then transferred to and merged...... with the questionnaire data in the DBDS database. RESULTS: The digital platform enables personalized questionnaires, presenting only questions relevant to the specific donor by hiding unneeded follow-up questions on screening question results. New versions of questionnaires are immediately available at all blood...... connection, but the level of security depends on the sensitivity of the data to be collected....

  16. Biological mothers may be dangerous blood donors for their neonates.

    Science.gov (United States)

    Elbert, C; Strauss, R G; Barrett, F; Goeken, N E; Pittner, B; Cordle, D

    1991-01-01

    Premature neonates require blood transfusions, and biological parents may wish to be directed donors. Biological mothers pose a potential danger because their plasma may contain antibodies that will react with blood cell antigens inherited by the infant from the father. We studied 25 healthy, pregnant women at the time of delivery for the presence of antibodies against red blood cell, leukocyte and platelet antigens. Mothers known to have red cell antibodies earlier in pregnancy were excluded, and no new red cell antibodies appeared at delivery. Antileukocyte and antiplatelet antibodies were found in 16 and 12% of mothers, respectively. Because these antibodies have the potential to cause adverse reactions when transfused passively, we suggest that either biological mothers not provide blood components containing plasma for their neonates or that maternal red cells and platelets be given as washed products. PMID:1853680

  17. Antibody to histo-blood group A antigen neutralizes HIV produced by lymphocytes from blood group A donors but not from blood group B or O donors

    DEFF Research Database (Denmark)

    Arendrup, M; Hansen, J E; Clausen, H;

    1991-01-01

    not inhibit the HTLV-IIIB/lyB or the HTLV-IIIB/lyO isolate. Specificity of the MAb-mediated inhibition was shown using A-antigen (tetrasaccharide). Thus, HIV infection of PBMC from donors with blood type A appears to induce expression of host-cell-encoded carbohydrate blood group A epitope on HIV which can...

  18. Electrocardiographic Abnormalities in Trypanosoma cruzi Seropositive and Seronegative Former Blood Donors

    OpenAIRE

    Ribeiro, Antonio L.; Sabino, Ester C; Marcolino, Milena S.; Salemi, Vera M. C.; Barbara M. Ianni; Fábio Fernandes; Luciano Nastari; André Antunes; Márcia Menezes; Cláudia Di Lorenzo Oliveira; Vandana Sachdev; Carrick, Danielle M.; Michael P Busch; Murphy, Eduard L.

    2013-01-01

    BACKGROUND: Blood donor screening leads to large numbers of new diagnoses of Trypanosoma cruzi infection, with most donors in the asymptomatic chronic indeterminate form. Information on electrocardiogram (ECG) findings in infected blood donors is lacking and may help in counseling and recognizing those with more severe disease. OBJECTIVES: To assess the frequency of ECG abnormalities in T.cruzi seropositive relative to seronegative blood donors, and to recognize ECG abnormalities associated w...

  19. Blood Donors on Medication - an Approach to Minimize Drug Burden for Recipients of Blood Products and to Limit Deferral of Donors.

    Science.gov (United States)

    Becker, Christian D K; Stichtenoth, Dirk O; Wichmann, Michael G; Schaefer, Christof; Szinicz, Ladislaus

    2009-01-01

    BACKGROUND: Blood products derived from donors on medication can contain drugs which might pose a risk for the recipients or influence the quality of the product itself. MATERIAL AND METHODS: To judge the eligibility of blood donors on medication, 4 drug classes have been formed with respect to their pharmacological properties, and blood products have been divided in accordance with their single-donor plasma contents. RESULTS: For drugs with dose-dependent pharmacodynamics, no deferral periods are necessary for donation of blood products containing less than 50 ml single-donor plasma for application to adults. Waiting periods of t(max) + 5 t(1/2) were calculated for the other blood products. Teratogenic drugs do not require special considerations (exception: retinoids, thalidomide and lenalidomide, dutasteride or finasteride with waiting periods for all blood products). A deferral period of t(max) + 24 t(1/2) is proposed for every blood product from blood donors on genotoxic drugs. Drugs without systemic effects can be neglected. Irreversible inhibitors of platelet function cause a 10-day waiting period if production of platelet concentrates is intended. CONCLUSION: Donors on medication are allowed to donate blood for blood products containing less than 50 ml plasma of a single donor, like red blood cell concentrates, for the use in adults without deferral periods, except those taking retinoids, thalidomide, lenalidomide, dutasteride, finasteride, or genotoxic drugs. PMID:20823991

  20. Handling low hemoglobin and iron deficiency in a blood donor population

    DEFF Research Database (Denmark)

    Magnussen, Karin; Ladelund, Steen

    2016-01-01

    BACKGROUND: Iron deficiency and blood donors with low hemoglobin (Hb) concentration are well-known challenges in any blood bank setting. In the Capital Region of Denmark, a new approach was adopted that centralized measurement of Hb, initiated ferritin (F) measurement, and established a center...... for donor Hb and iron. An algorithm was created based on Hb and F levels, which drove decisions on outreach by the donor Hb and iron resource team to the donor, including whether to provide iron supplementation or, on rare occasions, a referral to the donor's general practitioner. STUDY DESIGN AND METHODS......: The change in Hb for repeat donors was followed during the first 2 years of the intervention strategy, which included measurements of F and offering intermittent iron supplementation to some of the donors. RESULTS: In 2 years, 62,663 blood donors donated 193,288 units of blood and 318 donors gave 754...

  1. Sociodemographic and behavioral characteristics of HIV antibody-positive blood donors.

    Science.gov (United States)

    Cleary, P D; Singer, E; Rogers, T F; Avorn, J; Van Devanter, N; Soumerai, S; Perry, S; Pindyck, J

    1988-08-01

    This paper describes the sociodemographic and behavioral characteristics of 173 blood donors who were confirmed by Western blot tests to have antibodies to human immunodeficiency virus (HIV), the etiologic agent for acquired immunodeficiency syndrome (AIDS). Seropositive donors were predominantly young, unmarried, and male, and major risk factors could be identified for almost all donors. However, more than 20 per cent of the study participants were women, and many participants were not aware that they were at risk of infection. The heterogeneity of the study population, the lack of awareness among many subjects of risk factors and self-exclusion procedures, and the high level of distress among many subjects after notification, emphasize the need for intensive, well-designed education and support programs. PMID:3389433

  2. Legionella pneumophila Seropositivity-Associated Factors in Latvian Blood Donors

    OpenAIRE

    Olga Valciņa; Daina Pūle; Irina Lucenko; Dita Krastiņa; Žanete Šteingolde; Angelika Krūmiņa; Aivars Bērziņš

    2015-01-01

    Continuous environmental exposure of humans to Legionella may induce immune responses and generation of antibodies. The aim of this study was to investigate the seroprevalence of Legionella pneumophila serogroups (SG) 1–6 in the general healthy population and identify the associated host-related and environmental risk factors. L. pneumophila SG 1–6 seroprevalence among a total of 2007 blood samples collected from healthy donors was 4.8%. Seroprevalence was higher in women (5.9%) than men (3.3...

  3. Cardiovascular and demographic characteristics in whole blood and plasma donors: results from the Donor InSight study

    NARCIS (Netherlands)

    Atsma, Femke; Veldhuizen, Ingrid; Vegt, de Femmie; Doggen, Carine; Kort, de Wim

    2011-01-01

    BACKGROUND: Within blood establishments little comparative information is available about donors versus the general population. In this study, a description of the donor pool was made in terms of demographic factors and cardiovascular risk factors. The general Dutch population was used as a referenc

  4. Cardiovascular and demographic characteristics in whole blood and plasma donors: results from the Donor InSight study.

    NARCIS (Netherlands)

    Atsma, F.; Veldhuizen, I.; Vegt, F. de; Doggen, C.J.; Kort, W. de

    2011-01-01

    BACKGROUND: Within blood establishments little comparative information is available about donors versus the general population. In this study, a description of the donor pool was made in terms of demographic factors and cardiovascular risk factors. The general Dutch population was used as a referenc

  5. Seroepidemiology of human T-cell lymphotropic virus type-I in blood donors of Northeastern Iran, Sabzevar

    Directory of Open Access Journals (Sweden)

    Mahtab Maghsudlu

    2015-01-01

    Full Text Available Background and Objectives: Human T-cell lymphotropic virus type-I (HTLV-I infection is considered as a public health challenge in endemic areas. The virus is associated with severe diseases, such as adult T-cell leukemia/lymphoma, and HTLV-I-associated myelopathy/tropical spastic paraparesis. One of the major routes of the HTLV-I transmission includes blood transfusion. Sabzevar is located in the endemic region of HTLV-I infection. The aim of the present study was to determine the seroprevalence of HTLV-I infection in the blood donors in Sabzevar. Materials and Methods: A total of 35,067 blood donors in Sabzevar from March 2009 to April 2012 who were screened with HTLV-I on the enzyme-linked immunosorbent assay screening test were included in this survey. Reactive samples that confirmed by western blot were considered to be seropositive cases. The required data were obtained from blood donors′ database of blood transfusion service. Results: The overall prevalence of HTLV-1 based on the positive result of western blot test was 0.14%. The seropositive donors aged 17-59 years with a mean age of 38.10 ± 11.82. The prevalence rates of HTLV-I infection in 3 years of study were 0.19%, 0.14%, and 0.09%, respectively. A significant relation between age, sex, educational level, and history of blood donation was observed with seropositivity of HTLV-I. Conclusion: The improvement of donor selection and laboratory screening caused a decline in the prevalence of infection in blood donors. Given the lower prevalence of infection in regular donors with lower age and higher educational level, more efforts should be done to attract blood donors from these populations.

  6. Blood donor selection and deferral pattern as an important tool for blood safety in a tertiary care hospital

    Directory of Open Access Journals (Sweden)

    Manisha Shrivastava

    2016-01-01

    Full Text Available Context: Blood donor selection criteria based on science and regulatory rules influence donor as well recipient safety and demographics. Aim: To evaluate and analyze the blood donor deferral pattern and its causes among blood donors in a tertiary care hospital blood bank and to review its influence on blood safety. Settings and Design: Hospital based blood bank, retrospective analysis. Subjects and Methods: The data available as donor deferral record over a period of 13 years from 2001 to 2013 was analyzed. Results: The blood donor deferral rate was 11.5%, the deferral rate in various categories was 4.8%, 4.7%, 1.6%, and 0.3% in Category 1, Category 2, Category 3, and Category 4, respectively. The majority of deferrals were temporary deferrals (62.8% of young donors. The maximum number of donors deferred (28.2% due to a history of jaundice (permanent followed by 19.4% due to low hemoglobin (temporary. History of malaria, intake of medicines, infections, underweight, last blood donation within 3 months (temporary deferral, and history of heart and lung diseases, diabetes, and with suspicious identity (permanent deferral were other major causes identified. Conclusion: The pattern of donor deferral identified is an important tool for blood safety and also provides key areas to focus on a region or policy formulation nationally for donor selection as well ensure donor safety. The value of determining donor deferral pattern by the categories described is in calling back donors deferred due to temporary reasons and can help retain pool of motivated blood donors.

  7. Rate of seroconversion in repeat blood donors at the national blood centre, kuala lumpur.

    Science.gov (United States)

    Nafishah, A; Asiah, M Nor; Syimah, A T Nur; Mohd Zahari, T H; Yasmin, A; Normi, M; Anza, E; Shahnaz, M; Narazah, M Y

    2014-06-01

    The World Health Organization (WHO) recommend that all donated blood are to be screened for at least three viral infections [human immunodeficiency virus (HIV), hepatitis C virus (HCV), hepatitis B virus (HBV)]. The National Blood Centre, Kuala Lumpur (NBCKL) aims to reduce transfusion transmitted infections (TTI) as it still remains as one of the major risk for blood transfusion. A cross sectional study was conducted at the National Blood Centre, Kuala Lumpur from 1st January 2009 to 31st June 2010. Data from 581,020 donors were analyzed from year 2004 to 2008. All data were retrieved from NBCKL Blood Bank Information System (BBIS). A total of 201 repeat donors were included in the study based on the inclusion criteria but only 132 repeat donors agreed to participate. Information on sociodemographic, risk factors, knowledge of donors and high risk behavior were extracted from standardize questionnaire. Data were analyzed using SPSS version 14.0. The aim of this study was to determine the predictors of the seropositive infectivity among repeat blood donors at the NBCKL. The results showed Syphilis accounts for the highest and increasing seroconversion rate among other infections from 20.83 % in year 2004 to 44.6 % in year in year 2008. HIV and HCV infection also showed increasing seroconversion rate in 5 years' time from 6.41 % in year 2004 to 17.54 % in year 2008 and 4.8 % in year 2004 to 5.94 % in year 2008 respectively. However, HBV infection alone showed a decreasing seroconversion rate from 20.83 % in year 2004 to 10.4 % in year 2008. Level of donors' awareness regarding high risk factors (activities or behaviour) can lead to higher risk of TTI with significant p value in this predictors model(p < 0.05). Repeat blood donors with high risk activities are more likely to have seropositive results for HBV, HIV and Syphilis. This study found that the frequency of HCV seropositivity is higher among lapsed donor. Socio demographic factors such as male and

  8. The Frequency of Hepatitis B and C Among Volunteer Blood Donors in Balochistan

    Directory of Open Access Journals (Sweden)

    Zafar Ahmad Khan

    2007-08-01

    Full Text Available Background and Aims: In this cross-sectional study, the frequency of hepatitis B and C among volunteer blood donors in blood banks of Sandeman Provincial & Lady Duffren Hospitals, Quetta, Pakistan was estimated.Methods: 1474 blood donors were selected by convenient sampling. After obtaining informed consent, brief history and examination were done. They were tested for HBsAg and anti-HCV antibodies in laboratory. The collected data analyzed by SPSS 12.0 and results shown in frequencies and percentages.Results: Out of 1474 blood donors, 1284 (87.1% were males and 190 (12.9% were females. The mean age was 25 (range: 16-49 years. 71 (4.8% were positive for HBsAg (63 males & 8 females, 26 (1.8% for anti-HCV (21 males & 5 females and 4 (0.27% were positive for both (3 males & 1 female. Most of them belonged to ages 21-40 years.Conclusions: The frequency of hepatitis B is same as that of other part of the country contrary to the belief that Balochistan is a high prevalent region for hepatitis B as compare to the rest of the country, while the frequency of hepatitis C is less than expected. Health education and vaccination for hepatitis B should be encouraged and more vigilant efforts should be done.

  9. Association of ABO and Rh Blood Groups to Blood-Borne Infections among Blood Donors in Tehran-Iran.

    Directory of Open Access Journals (Sweden)

    Fatemeh Mohammadali

    2014-07-01

    Full Text Available The aim of this study was to investigate the prevalence of hepatitis B, hepatitis C, HIV and syphilis infections in blood donors referred to Tehran Blood Transfusion Center (TBTC, and determine any association between blood groups and blood- borne infections between the years of 2005 and 2011.This was a retrospective study conducted at TBTC. All of the donor serum samples were screened for HBV, HCV, HIV and syphilis by using third generation ELISA kits and RPR test. Initial reactive samples were tested in duplicate. Confirmatory tests were performed on all repeatedly reactive donations. Blood group was determined by forward and reverse blood grouping. The results were subjected to chi square analysis for determination of statistical difference between the values among different categories according to SPSS program.Overall, 2031451 donor serum samples were collected in 2005-2011. Totally, 10451 were positive test for HBV, HCV, HIV and syphilis. The overall seroprevalence of HBV, HCV, HIV, and syphilis was 0.39%, 0.11%, 0.005%, and 0.010%, respectively. Hepatitis B and HIV infections were significantly associated with blood group of donors (P 0.05.Compared with neighboring countries and the international standards, prevalence of blood-borne infections is relatively low.

  10. Evaluation of blood donor deferral causes in a tertiary hospital, South India

    OpenAIRE

    John, Fred; Varkey, Mary Rithu

    2015-01-01

    Aims: This study aims to analyze the donor deferral rates, various causes of deferrals and to take proper referral and follow up measures to decrease the temporary deferral rate by which we could increase the pool of voluntary donors without compromising on the quality of the blood and safety to the recipient.Methods: A two year retrospective study of donors, carried out in a tertiary hospital in south India, and also includes voluntary donors from outdoor camps conducted by blood bank during...

  11. A retrospective cohort study of blood hemoglobin levels in blood donors and competitive rowers

    DEFF Research Database (Denmark)

    Johansson, P. I.; Ullum, H.; Jensen, Kurt;

    2009-01-01

    .3% of the females demonstrated values above the recommended limit for athletic competition. Thus, the prevalence of a high hemoglobin value was greater in the rowers, of both gender, than in the candidate blood donors (P... people and especially in competition athletes....

  12. Attitude and opinion of volunteer blood donors regarding access to eugenic

    Directory of Open Access Journals (Sweden)

    Konstantina Chatzilaou

    2009-02-01

    Full Text Available PURPOSE: The assessment of the attitude and views of blood donors in relation to eugenics. As well as the relation of eugenics to several demographical factors such as educational level, sex age and religious beliefs.MATERIAL AND METHOD: The subjects of study were 252 blood donors, who voluntarily gave blood and its byproducts in a Blood Donation Center in Athens. The data was collected through an anonymous questionnaire especially designed for the purpose of this study.RESULTS: The overwhelming majority of volunteer blood-donors agree in percentage greater than 50%, that society will probably never offer sufficient support to individuals with disabilities and that a woman should be submitted to prenatal control to determine if she medically advised in relation to her age and family background. 79% agrees that they would want to learn more about the moral questions that arise from the application of genetics to human.Independent variables that influence the attitude towards eugenics were found to be the importance of religion, the level of education, the sex and the number of children.Also, there was a connection of religion and the number of children with the knowledge of moral questions on genetics.CONCLUSIONS: Prenatal control should be carried out only when serious genetic illnesses or illnesses whose treatment is imperative to start early are expected. The attitude of citizens towards individuals with disabilities should change radically so that the biases and social stereotypes are raised. A better provision of information of the public on the moral questions that derive from the application of genetics to humans is also suggested.

  13. National blood requirement, serum ALT and hepatitis in Ethiopian blood donors.

    Science.gov (United States)

    Zawde, D; Sisay, Y

    1991-10-01

    To appraise the national blood requirement and supply, and to determine the impact of alanine aminotransferase (ALT) and hepatitis B surface antigen (HBsAg) screening on the blood supply, 407 random blood donor sera were tested for HBsAg, human immunodeficiency virus (HIV), and ALT activity. HBsAg and anti-HIV antibody were determined by the enzyme linked immunosorbent assay (ELISA) technique using Hepanostica and Welcozyme kits, respectively. The Western Blot test was performed to confirm anti-HIV positive sera by the ELISA technique. ALT was determined by an automated photometer using ALAT kits and serologic testing for syphilis was done by the rapid plasma reagin (RPR) test. The amount of blood required in Ethiopia and the actual supply was calculated on the basis of the number and type of hospital beds in Addis Abeba and the amount of blood transfusions in units per hospital bed. The results showed that the combined donor and unit rejection rate was 34.6%. The annual blood requirement was 7 units for emergency and 4 units for nonemergency beds. The national blood requirement in 1989 was 64,350-80,000 units, but the supply met only a third of the requirement. The mean and 2SD cut off ALT levels were 28 and 69 IU/L, respectively. ALT was elevated in 9.1% of HBsAg positive but apparently healthy donors, while HBsAg screening eliminated 25% of those with elevated ALT activity. This data suggests that there is a serious blood shortage in Ethiopia and that the currently supplied blood is relatively unsafe in terms of hepatitis.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:1659534

  14. Improving health profile of blood donors as a consequence of transfusion safety efforts

    DEFF Research Database (Denmark)

    Edgren, Gustaf; Tran, Trung Nam; Hjalgrim, Henrik;

    2007-01-01

    BACKGROUND: Transfusion safety rests heavily on the health of blood donors. Although they are perceived as being healthier than average, little is known about their long-term disease patterns and to which extent the blood banks' continuous efforts to optimize donor selection has resulted in impro......BACKGROUND: Transfusion safety rests heavily on the health of blood donors. Although they are perceived as being healthier than average, little is known about their long-term disease patterns and to which extent the blood banks' continuous efforts to optimize donor selection has resulted...

  15. Effect of autologous blood donation on the central venous pressure, blood loss and blood transfusion during living donor left hepatectomy

    Institute of Scientific and Technical Information of China (English)

    Bruno Jawan; Shih-Hor Wang; Chih-Che Lin; Tsan-Shiun Lin; Yueh-Wei Liu; Chao-Long Chen; Yu-Fan Cheng; Chia-Chi Tseng; Yaw-Sen Chen; Chih-Chi Wang; Tung-Liang Huang; Hock-Liew Eng; Po-Ping Liu; King-Wah Chiu

    2005-01-01

    AIM: Autologous blood donation (ABD) is mainly used to reduce the use of banked blood. In fact, ABD can be regarded as acute blood loss. Would ABD 2-3 d before operation affect the CVP level and subsequently result in less blood loss during liver resection was to be determined.METHODS: Eighty-four patients undergoing living donor left hepatectomy were retrospectively divided as group Ⅰ (GⅠ)and group Ⅱ (GⅡ) according to have donated 250-300 mL blood 2-3 d before living donor hepatectomy or not. The changes of the intraoperative CVP, surgical blood loss,blood products used and the changes of perioperative hemoglobin (Hb) between groups were analyzed and compared by using Mann-Whitney Utest.RESULTS: The results show that the intraoperative CVP changes between GⅠ (n = 35) and GⅡ (n = 49) up to graft procurement were the same, subsequently the blood loss,but ABD resulted in significantly lower perioperative Hb levels in GⅠ.CONCLUSION: Since none of the patients required any blood products perioperatively, all the predonated bloods were discarded after the patients were discharged from the hospital, It indicates that ABD in current series had no any beneficial effects, in term of cost, lowering the CVP, blood loss and reduce the use of banked blood products, but resulted in significant lower Hb in perioperative period.

  16. Adverse Reactions in Allogeneic Blood Donors: A Tertiary Care Experience from a Developing Country

    Directory of Open Access Journals (Sweden)

    Sadia Sultan

    2016-03-01

    Full Text Available Objectives: Fragmented blood transfusion services along with an unmotivated blood donation culture often leads to blood shortage. Donor retention is crucial to meet the increasing blood demand, and adverse donor reactions have a negative impact on donor return. The aim of this study was to estimate adverse donor reactions and identify any demographic association.   Methods: We conducted a prospective study between January 2011 and December 2013. A total of 41,759 healthy donors were enrolled. Professionally trained donor attendants drew blood and all donors were observed during and following donation for possible adverse events for 20 minutes. Blood donors were asked to report if they suffered from any delayed adverse consequences.   Results: Out of 41,759 blood donors, 537 (1.3% experienced adverse reactions. The incidence was one in every 78 donations. The mean age of donors who experienced adverse events was 26.0±6.8 years, and all were male. Out of 537 donors, 429 (80% developed vasovagal reaction (VVR, 133 (25% had nausea, 63 (12% fainted, 35 (6% developed hyperventilation, 9 (2% had delayed syncope, and 9 (2% developed hematoma. Arterial prick, nerve injury, cardiac arrest, and seizures were not observed. Donors aged less than < 30 years and weighing < 70 kg were significantly associated with VVR, hyperventilation, and nausea (p < 0.005. Undergraduates and Urdu speaking donors also had a significant association with fainting and nausea, respectively (p < 0.05.   Conclusion: The prevalence of adverse events was low at our tertiary center. A VVR was the predominant adverse reaction and was associated with age and weight. Our study highlights the importance of these parameters in the donation process. A well-trained and experienced phlebotomist and pre-evaluation counseling of blood donors could further minimize the adverse reactions.

  17. The National Heart, Lung, and Blood Institute retrovirus epidemiology donor studies (Retrovirus Epidemiology Donor Study and Retrovirus Epidemiology Donor Study-II): twenty years of research to advance blood product safety and availability.

    Science.gov (United States)

    Kleinman, Steven; King, Melissa R; Busch, Michael P; Murphy, Edward L; Glynn, Simone A

    2012-10-01

    The Retrovirus Epidemiology Donor Study (REDS), conducted from 1989 to 2001, and the REDS-II, conducted from 2004 to 2012, were National Heart, Lung, and Blood Institute-funded, multicenter programs focused on improving blood safety and availability in the United States. The REDS-II also included international study sites in Brazil and China. The 3 major research domains of REDS/REDS-II have been infectious disease risk evaluation, blood donation availability, and blood donor characterization. Both programs have made significant contributions to transfusion medicine research methodology by the use of mathematical modeling, large-scale donor surveys, innovative methods of repository sample storage, and establishing an infrastructure that responded to potential emerging blood safety threats such as xenotropic murine leukemia virus-related virus. Blood safety studies have included protocols evaluating epidemiologic and/or laboratory aspects of human immunodeficiency virus, human T-lymphotropic virus 1/2, hepatitis C virus, hepatitis B virus, West Nile virus, cytomegalovirus, human herpesvirus 8, parvovirus B19, malaria, Creutzfeldt-Jakob disease, influenza, and Trypanosoma cruzi infections. Other analyses have characterized blood donor demographics, motivations to donate, factors influencing donor return, behavioral risk factors, donors' perception of the blood donation screening process, and aspects of donor deferral. In REDS-II, 2 large-scale blood donor protocols examined iron deficiency in donors and the prevalence of leukocyte antibodies. This review describes the major study results from over 150 peer-reviewed articles published by these 2 REDS programs. In 2011, a new 7-year program, the Recipient Epidemiology and Donor Evaluation Study-III, was launched. The Recipient Epidemiology and Donor Evaluation Study-III expands beyond donor-based research to include studies of blood transfusion recipients in the hospital setting and adds a third country, South Africa

  18. Algorithm for recall of HIV reactive Indian blood donors by sequential immunoassays enables selective donor referral for counseling

    Directory of Open Access Journals (Sweden)

    Thakral B

    2006-01-01

    Full Text Available Background: HIV/AIDS pandemic brought into focus the importance of safe blood donor pool. Aims: To analyze true seroprevalence of HIV infection in our blood donors and devise an algorithm for donor recall avoiding unnecessary referrals to voluntary counseling and testing centre (VCTC. Materials and Methods: 39,784 blood units were screened for anti-HIV 1/2 using ELISA immunoassay (IA-1. Samples which were repeat reactive on IA-1 were further tested using two different immunoassays (IA-2 and IA-3 and Western blot (WB. Based on results of these sequential IAs and WB, an algorithm for recall of true HIV seroreactive blood donors is suggested for countries like India where nucleic acid testing or p24 antigen assays are not mandatory and given the limited resources may not be feasible. Results: The anti-HIV seroreactivity by repeat IA-1, IA-2, IA-3 and WB were 0.16%, 0.11%, 0.098% and 0.07% respectively. Of the 44 IA-1 reactive samples, 95.2% (20/21 of the seroreactive samples by both IA-2 and IA-3 were also WB positive and 100% (6/6 of the non-reactive samples by these IAs were WB negative. IA signal/cutoff ratio was significantly low in biological false reactive donors. WB indeterminate results were largely due to non-specific reactivity to gag protein (p55. Conclusions: HIV seroreactivity by sequential immunoassays (IA-1, IA-2 and IA-3; comparable to WHO Strategy-III prior to donor recall results in decreased referral to VCTC as compared to single IA (WHO Strategy-I being followed currently in India. Moreover, this strategy will repose donor confidence in our blood transfusion services and strengthen voluntary blood donation program.

  19. Legionella pneumophila Seropositivity-Associated Factors in Latvian Blood Donors

    Directory of Open Access Journals (Sweden)

    Olga Valciņa

    2015-12-01

    Full Text Available Continuous environmental exposure of humans to Legionella may induce immune responses and generation of antibodies. The aim of this study was to investigate the seroprevalence of Legionella pneumophila serogroups (SG 1–6 in the general healthy population and identify the associated host-related and environmental risk factors. L. pneumophila SG 1–6 seroprevalence among a total of 2007 blood samples collected from healthy donors was 4.8%. Seroprevalence was higher in women (5.9% than men (3.3% and in areas with a larger number of inhabitants, ranging from 3.5% in rural regions to 6.8% in the capital, Riga. Blood samples from inhabitants of apartment buildings tested positive for L. pneumophila in more cases (5.8% compared to those from inhabitants of single-family homes (2.7%. Residents of buildings with a municipal hot water supply system were more likely to be seropositive for L. pneumophila (OR = 3.16, 95% CI 1.26–7.91. Previous episodes of fever were additionally identified as a risk factor (OR = 2.42, 95% CI 1.43–4.1. In conclusion, centralized hot water supply, female gender and previous episodes of fever were determined as the main factors associated with L. pneumophila seropositivity in our study population.

  20. Legionella pneumophila Seropositivity-Associated Factors in Latvian Blood Donors

    Science.gov (United States)

    Valciņa, Olga; Pūle, Daina; Lucenko, Irina; Krastiņa, Dita; Šteingolde, Žanete; Krūmiņa, Angelika; Bērziņš, Aivars

    2015-01-01

    Continuous environmental exposure of humans to Legionella may induce immune responses and generation of antibodies. The aim of this study was to investigate the seroprevalence of Legionella pneumophila serogroups (SG) 1–6 in the general healthy population and identify the associated host-related and environmental risk factors. L. pneumophila SG 1–6 seroprevalence among a total of 2007 blood samples collected from healthy donors was 4.8%. Seroprevalence was higher in women (5.9%) than men (3.3%) and in areas with a larger number of inhabitants, ranging from 3.5% in rural regions to 6.8% in the capital, Riga. Blood samples from inhabitants of apartment buildings tested positive for L. pneumophila in more cases (5.8%) compared to those from inhabitants of single-family homes (2.7%). Residents of buildings with a municipal hot water supply system were more likely to be seropositive for L. pneumophila (OR = 3.16, 95% CI 1.26–7.91). Previous episodes of fever were additionally identified as a risk factor (OR = 2.42, 95% CI 1.43–4.1). In conclusion, centralized hot water supply, female gender and previous episodes of fever were determined as the main factors associated with L. pneumophila seropositivity in our study population. PMID:26703696

  1. Signalment and Blood Types in Cats Being Evaluated as Blood Donors at Two Italian University Blood Banks

    OpenAIRE

    Eva Spada; Arianna Miglio; Daniela Proverbio; Maria Teresa Antognoni; Giada Bagnagatti de Giorgi; Elisabetta Ferro; Vittorio Mangili

    2014-01-01

    Data from potential feline blood donors presented at two university blood banks in Italy were recorded. Blood typing was performed using an immunochromatographic method. Over the three years of the study 357 cats representing 15 breeds, 45.3% female and 54.7% male, with a mean age of 3.8 years were evaluated. Of these 90.5% were blood type A, 5.6% type B, and 3.9% type AB. The majority of the cats (54.6%) were European DSH (92.3% were type A, 5.1% type B, and 2.6% type AB), and 21% were Maine...

  2. Frequency of thalassemia carrier and Hb variant and the quality of stored donor blood

    OpenAIRE

    Eva A. Maharani; Yuyun S.M. Soedarmono; Nainggolan, Ita M.

    2015-01-01

    Background: This study was aimed to determine the frequency of thalassemia and Hb variant in blood donor. In addition, we also wanted to know the quality of blood from the donor up to seven days of storage, by calculating percentage of hemolysis in vitro.Methods: This cross-sectional study was conducted on 138 blood donor specimens at Red Cross Blood Centre Unit in Jakarta. All specimens were tested for thalassemia and Hb variant by complete blood count (CBC) and Hb analysis with HPLC method ...

  3. Determination of hepatitis C virus genotypes among blood donors in Ahvaz, Iran

    OpenAIRE

    Farshadpour F; Makvandi M; Samarbafzadeh A; Jalalifar M

    2010-01-01

    This study aims to determine the genotypes of hepatitis C virus (HCV) among blood donors at Ahvaz Blood Transfusion Centre. Blood samples were taken from 2376 blood donors - 1795 (75.54%) male and 581(24.45%) female - who referred to Ahvaz Blood Transfusion Centre during 2007-2008. Detection of anti-HCV antibody for all the donors was carried out by ELISA and the confirmatory RIBA tests. HCV RT-PCR followed by RFLP test was carried out for anti-HCV positive samples. Out of 237...

  4. Infectivity of blood products from donors with occult hepatitis B virus infection

    DEFF Research Database (Denmark)

    Allain, Jean-Pierre; Mihaljevic, Ivanka; Gonzalez-Fraile, Maria Isabel;

    2013-01-01

    Occult hepatitis B virus (HBV) infection (OBI) is identified in 1:1000 to 1:50,000 European blood donations. This study intended to determine the infectivity of blood products from OBI donors.......Occult hepatitis B virus (HBV) infection (OBI) is identified in 1:1000 to 1:50,000 European blood donations. This study intended to determine the infectivity of blood products from OBI donors....

  5. A behavior model for blood donors and marketing strategies to retain and attract them

    OpenAIRE

    Covadonga Aldamiz-echevarria; Maria Soledad Aguirre-Garcia

    2014-01-01

    Objective: analyze and propose a theoretical model that describes blood donor decisions to help staff working in blood banks (nurses and others) in their efforts to capture and retain donors. Methods: analysis of several studies on the motivations to give blood in Spain over the last six years, as well as past literature on the topic, the authors' experiences in the last 25 years in over 15 Non Governmental Organizations with different levels of responsibilities, their experiences as blood do...

  6. Risk Factors and Screening for Trypanosoma cruzi Infection of Dutch Blood Donors

    OpenAIRE

    Slot, Ed; Hogema, Boris M; Molier, Michel; BART, Aldert; Hans L Zaaijer

    2016-01-01

    Background Blood donors unaware of Trypanosoma cruzi infection may donate infectious blood. Risk factors and the presence of T. cruzi antibodies in at-risk Dutch blood donors were studied to assess whether specific blood safety measures are warranted in the Netherlands. Methodology Birth in a country endemic for Chagas disease (CEC), having a mother born in a CEC, or having resided for at least six continuous months in a CEC were considered risk factors for T. cruzi infection. From March thro...

  7. Assessing Acceptability of Short Message Service Based Interventions towards Becoming Future Voluntary Blood Donors

    OpenAIRE

    Sana Saleem; Anum Wasim; Sidra Sabih; Ayisha Farooq Khan; Madiha Hasan Rizvi; Umaima Ayesha Jillani; Mujtaba Jamal Syed; Madiha Mumtaz; Yasmeen Mumtaz; Abdul Moid Shehzad; Om Dawani; Saima Khan; Sheheryar Munir; Nava Asad; Abdul Nafey Kazi

    2014-01-01

    All blood bank services, especially those of developing countries, face a major shortfall of blood donations due to lack of voluntary blood donors. Our study aims to evaluate the acceptability of Short Message Service based interventions towards becoming voluntary blood donors among medical university students of Karachi, Pakistan. Methods. A total of 350 medical students were approached in medical universities of Karachi, Pakistan, using a nonprobability convenient sampling technique. Data c...

  8. Seroprevalence of HIV, HBV, HCV and syphilis in blood donors in Southern Haryana.

    Science.gov (United States)

    Arora, Dimple; Arora, Bharti; Khetarpal, Anshul

    2010-01-01

    Blood transfusion is an important mode of transmission of infections to recipients. The aim of the study was to assess the prevalence of transfusion-transmissible infections among blood donors. For this, a 3.5-year retrospective study, from October 2002 to April 2006 was conducted at the blood transfusion centre of Maharaja Agrasen Medical College, Agroha (Hisar) Haryana. Donors were screened for seroprevalence of HIV, HBV, HCV and syphilis. A total of 5849 donors were tested, out of which 4010 (68.6%) were replacement donors and 1839 (31.4%) were voluntary donors. The seroprevalence of HIV was 0.3% in the donors. No voluntary donor was found to be positive for HIV. The low sero-positivity among donors is attributed to pre-donation counseling in donor selection. The seroprevalence of HBV, HCV and syphilis was 1.7%, 1.0% and 0.9% respectively in total donors. The seroprevalence of hepatitis and syphilis was more in replacement donors as compared to voluntary donors. PMID:20551540

  9. Seroprevalence of human T-cell lymphotropic virus-1/2 in blood donors in northern pakistan: implication for blood donor screening

    International Nuclear Information System (INIS)

    To determine the seroprevalence of Human T-cell Lymphotropic Virus-1/2 (HTLV-1/2) in blood donors in Northern Pakistan. Study Design: Descriptive study. Place and Duration of Study: Armed Forces Institute of Transfusion, Rawalpindi, from July to August 2013. Methodology:A total of 2100 blood donors were screened for anti-HTLV-1/2 antibodies during the study period, in a pool of six, on a highly sensitive, Chemiluminiscent Microparticle Immunoassay (CMIA) based system. The screening test reactive donors were recalled, counseled and interviewed, and a fresh sample was obtained for confirmatory testing. Confirmation was performed using additional immunoassays including Line Immunoassay (LIA); with additional testing for HTLV-1 pvDNAPCR. Frequency and percentages were determined. Results: Four donors (0.19%) were repeatedly screening test-reactive and were subsequently confirmed to be HTLV-1 infected by line immunoassay and HTLV-1 pvDNAPCR. All four donors were male with mean age of 27 ± 6.27 years. Two (50%) of the positive donors gave history of Multiple Sexual Partners (MSP). Conclusion: HTLV-1 seroprevalence in Northern Pakistan blood donors was determined to be 0.19%. Large scale studies, including the cost effectiveness of screening blood donations for anti-HTLV-1/2 in Pakistan, are recommended. (author)

  10. ABO/RH DISTRIBUTION PATTERN AND PREVALENCE OF IRREGULAR ANTIBODIES IN HEALTHY BLOOD DONORS OF UTTARAKHAND

    Directory of Open Access Journals (Sweden)

    Brijesh Thakur

    2016-07-01

    Full Text Available BACKGROUND Blood group plays a vital role in transfusion safety, understanding genetics, inheritance pattern and disease susceptibility. This study is aimed to determine distribution pattern of ABO and Rh blood group, incidence and identification of irregular antibodies among blood donors of Uttarakhand. METHOD ABO/Rh blood grouping was performed by test tube agglutination method (both cell and serum grouping using antisera A, B and Rh from Tulip and Orthodiagnostics. Donors’ plasma were screened for irregular antibodies by indirect antiglobulin technique using Bio-Rad ID-Card “LISS/Coombs” and test cell reagents “ID-DiaCell” via gel centrifugation. RESULTS Out of total 4573 included blood donors, 88.2% were males. The maximum blood donors (77.6% were in 18-25 years of age group. The commonest ABO blood group present was B (36.1% followed by O (29.1%, A (24.8% and AB (10% in blood donors; while in Rhesus system, 95.0% donors were Rh-positive and 5.0% donors were Rh negative. Screening of donors’ plasma did not reveal any detectable irregular antibody. CONCLUSION The study has a significant implication in suggesting the distribution pattern of ABO/Rh blood group typing in the region, but routine irregular antibody screening in healthy blood donors may not be fruitful in this region keeping cost effectiveness and absence of previous sensitization in mind

  11. Prevalence of hepatitis B surface antigen (HBsAg) in blood donors from Bombay.

    Science.gov (United States)

    Satoskar, A; Ray, V

    1992-01-01

    Analysis of serum samples from 3104 blood donors from Bombay screened for hepatitis B surface antigen (HBsAg) by ELISA. HBsAg was detected in 4.7% of the subjects. Relatives showed a significantly higher prevalence of HBsAg than volunteer donors. There was no significant association between HBsAg positivity and a particular blood group.

  12. Transfusion safety in francophone African countries: An analysis of strategies for the medical selection of blood donors

    OpenAIRE

    Tagny, CT; Kouao, MD; Touré, H.; Gargouri, J; Fazul, AS

    2011-01-01

    BACKGROUND: The goal of selecting a healthy blood donor is to safeguard donors and reduce the risks of infections and immunologic complications for recipients. STUDY DESIGN AND METHODS: To evaluate the blood donor selection process, a survey was conducted in 28 blood transfusion centers located in 15 francophone African countries. Data collected included availability of blood products, risk factors for infection identified among blood donor candidates, the processing of the information collec...

  13. Antibody to histo-blood group A antigen neutralizes HIV produced by lymphocytes from blood group A donors but not from blood group B or O donors

    DEFF Research Database (Denmark)

    Arendrup, M; Hansen, J E; Clausen, H;

    1991-01-01

    for virus neutralization by the monoclonal antibody (MAb) AH16 directed against the blood group A epitope. MAb AH16 was previously shown to inhibit cell-free virus infection using HTLV-IIIB propagated in H9 cells. AH16 showed a concentration-dependent inhibition of the HTLV-IIIB/lyA isolate but did...... not inhibit the HTLV-IIIB/lyB or the HTLV-IIIB/lyO isolate. Specificity of the MAb-mediated inhibition was shown using A-antigen (tetrasaccharide). Thus, HIV infection of PBMC from donors with blood type A appears to induce expression of host-cell-encoded carbohydrate blood group A epitope on HIV which can......Three virus isolates HTLV-IIIB/lyA, HTLV-IIIB/lyB and HTLV-IIIB/lyO, obtained by passaging and propagating the HTLV-IIIB/H9 isolate in three separate cultures of mixed peripheral blood mononuclear cells (PBMC) from donors of blood type A, B or O, respectively, were tested for susceptibility...

  14. Determination of hepatitis C virus genotypes among blood donors in Ahvaz, Iran

    Directory of Open Access Journals (Sweden)

    Farshadpour F

    2010-01-01

    Full Text Available This study aims to determine the genotypes of hepatitis C virus (HCV among blood donors at Ahvaz Blood Transfusion Centre. Blood samples were taken from 2376 blood donors - 1795 (75.54% male and 581(24.45% female - who referred to Ahvaz Blood Transfusion Centre during 2007-2008. Detection of anti-HCV antibody for all the donors was carried out by ELISA and the confirmatory RIBA tests. HCV RT-PCR followed by RFLP test was carried out for anti-HCV positive samples. Out of 2376 blood donors, only 55 (2.3% male donors showed to be positive for HCV antibody by ELISA and RIBA tests out of which 45(1.8% donors were positive for RT-PCR test. Female donors were negative for HCV antibody. The result of HCV genotyping by RFLP test showed 24 (53.3% for 1a, 17 (37.7% for 3a (a and 4 (8.8% for 3a (b genotypes respectively. In conclusion, high prevalence of 53.3% HCV 1a genotype was observed among blood donors in Ahvaz city.

  15. Risk Factors for Hepatitis C Virus Infection among Blood Donors in Georgia

    International Nuclear Information System (INIS)

    Background: Growing awareness about the importance of blood safety for controlling the transmission of hepatitis C virus (HCV) has helped to decrease the spread of this virus in many settings. This study was conducted in order to evaluate potential risk factors for HCV infection among blood donors in Georgia. Methods: The study population consisted of 553 blood donors in three major Georgian cities; Tbilisi, the capital city and Batumi and Poti, naval port cities. Risk factors were examined using a behavior questionnaire. All blood samples were initially tested using 3rd generation anti-HCV enzyme-linked immunosorbent assays and confirmed using recombinant immunoblot assays and nucleic acid testing. Results: Forty-three blood donors, 7.8%, were confirmed HCV positive. Significant risk factors included: drug injection ever (OR: 42; 95% CI: 3.2-550.7); history of hepatitis (OR: 25.9; 95% CI: 4.6-145.5); history of a previous surgical procedure (OR: 148.4; 95% CI: 26.9-817.4); blood transfusion (OR: 25.9; 95% CI: 3.2-210.9). Conclusions: This study found a very high prevalence of HCV among blood donors in Georgia. The main risk factor for HCV infection in this population of blood donors was previous contact with contaminated blood or blood products. Reliable screening of donors and their blood is critical for controlling the further spread of HCV in Georgia

  16. Role of Medical Social Workers in Management of Anxiety and Stress Among Blood Donors with Transfusion Transmissible Infections

    Directory of Open Access Journals (Sweden)

    Umakanth Siromani

    2014-02-01

    Full Text Available Blood transfusion is a life-saving intervention and millions of lives are saved each year globally through this procedure. Unsafe transfusion practices put millions of people at risk of transfusion-transmissible infections (TTIs and it is mandatory to test the donated blood for blood borne infectious diseases. This is an alarming situation requiring immediate action in appropriate counseling of donors before and after testing of their blood. It is really a challenge for blood banks and motivators if a blood donor is positive for infectious diseases. What is the role of blood bank in helping or guiding the donors to overcome their anxiety and stress? How a medical social worker could psychologically support and guide them to act as cause ambassadors for voluntary blood donation? Guidance and counseling would help them to live positively. Health education, compassionate care and teaching coping mechanisms would encourage them in overcoming their stress and anxiety. [Natl J Med Res 2014; 4(1.000: 87-88

  17. Characterization of rubella seronegative females in the Zambian blood donor community

    Directory of Open Access Journals (Sweden)

    Mazyanga Lucy Mazaba

    2015-04-01

    Full Text Available Rubella is an acute, contagious viral infection caused by a teratogenic enveloped single-stranded RNA virus, rubella virus, a member of the togaviridae family. Though causing generally mild infections in children and adults, it is a disease of public health importance in pregnant women causing major problems including abortions, miscarriages and Congenital Rubella Syndrome in more than twenty per cent of the susceptible population. This study was carried out to determine the characteristics associated with rubella seronegativity amongst female blood donors in Zambia. Rubella-specific IgG antibody levels were measured in the blood serum. Proportions were compared using the Chi-squared test at the 5% significance level, and magnitudes of associations were determined using the odds ratio and its 95% confidence interval. Of the 124 female blood donors tested for rubella IgG 46.0% were aged less than 20 years. Overall, 66.7% of the participants had never been married. More than half (62.1% of the participants resided in rural areas of the country. Of the 114 participants with recorded level of education, 50.1% had at–least completed secondary school. Out of 43 participants with recorded current employment status, 44.2% were not working for pay. A total of 10 (8.1% participants were sero-negative to rubella IgG antibodies. No factors were associated with seronegativity. Protection against rubella through natural infection appears inadequate to protect the population, increasing the risk of CRS.

  18. Investigation on the Distribution of Regular Blood Donors in Urban Wenzhou%温州市区固定献血者分布调查

    Institute of Scientific and Technical Information of China (English)

    谢熙熙; 苏虹; 陈通; 陈李

    2012-01-01

    Objective To establish regular non-remunerated blood donors team through investigation on the urban regular blood donors in Wenzhou. Methods The relationship between donation times and the ages and blood types was analyzed, and the distribution of regular blood donors and blood components donors was contrasted. Results The difference of donation times among different aged regular donors is statistically significant (P 0.05 ). Occupation distribution and educational level distribution between regular blood donors and blood components donors were different. Conclusion Publicity should be strengthened, and identification of the target population of the fixed blood donors with components donors could improve the recruitment efficiency,consolidate and enlarge the regular blood donors population.%目的 通过对温州市区固定献血者人群分布调查,为建立一支固定献血者队伍提供依据.方法 分析献血次数与年龄、血型的关系,对固定献血者人群与其中成分献血者分布情况进行对比分析.结果 不同年龄固定献血者献血次数差异有统计学意义(P<0.05);不同血型固定献血者献血次数差异无有统计学意义(P>0.05).固定献血者与参与成分献血人群在职业与文化程度分布情况有差别.结论 加大宣传,明确招募固定献血者与成分献血者目标人群,可以提高固定献血者招募效率,巩固并扩大固定献血者队伍.

  19. Effects of plateletpheresis on blood coagulation parameters in healthy donors at National Blood Centre, Kuala Lumpur, Malaysia.

    Science.gov (United States)

    Siti Nadiah, A K; Nor Asiah, M; Nur Syimah, A T; Normi, M; Anza, E; Aini, A Nor; Mohd Zahari, T H; Shahnaz, M; Faraizah, A K; Faisal, M A

    2013-12-01

    Plateletpheresis is a method used to remove platelet from the body either from random volunteer donors, patient's family members or HLA matched donors. A cross sectional study was carried out on 59 plateletpheresis donors aged between 18 and 55 years at National Blood Center (NBC), Kuala Lumpur. We compared the blood parameters before and after plateletpheresis and we found that the platelet count, FVIII, fibrinogen and thrombophilia markers anti-thrombin (AT), protein C and protein S were significantly reduced (p<0.05) with prolonged PT and APTT. There were significant changes in blood coagulation parameters but it is within acceptable range.

  20. Pre-birth selection of umbilical cord blood donors

    Science.gov (United States)

    Urciuoli, Patrizia; Passeri, Simona; Ceccarelli, Francesca; Luchetti, Barbara; Paolicchi, Aldo; Lapi, Simone; Nocchi, Francesca; Lamanna, Roberta; Iorio, Mariacarla; Vanacore, Renato; Mazzoni, Alessandro; Scatena, Fabrizio

    2010-01-01

    Background . The fact that only a small percentage of cord blood units (CBU) stored are actually used for transplantation contributes to raising the already high costs of their processing and cryopreservation. The identification of predictors allowing the early identification of suitable CBU would allow a reduction of costs for the collection, storage and characterisation of CBU with insufficient volume or cell numbers. In our bank we have adopted a cut-off value for using CBU of 8 × 108 nucleated cells and a volume ≥ 60 mL. Materials and methods In 365 banked CBU, we evaluated the correlation between neonatal/gestational parameters and laboratory data used to assess their quality. Results Biparietal diameter (BPD) and abdominal circumference were significantly and positively correlated with CBU volume (r2=0.12, p=0.0011 and r2=0.092, p=0.0063, respectively). Receiver operating characteristic (ROC) analysis showed that both parameters can be used to identify CBU with insufficient volume (BPD: area under the curve 0.69, 95% CI=0.57–0.82, p=0.004; abdominal circumference: area under the curve 0.67, 95% CI=0.54–0.79, p<0.01). BPD and head circumference, but not abdominal circumference or femoral length, were positively correlated with white blood cell (WBC) count (r2=0.215, p=0.031, and r2=0.299, p=0.015, respectively). Abdominal circumference, but not BPD, head circumference or femoral length, was statistically significantly correlated with the number of CD34+ cells in the CBU. Weight at birth and placental weight were positively correlated with WBC count, blood volume, CD34+ cell count, total colony-forming units and burst-forming units. Conclusion . Pre-birth assessment of BPD might allow the selection of donors who would yield CBU of sufficient volume and WBC count and avoid the costs of collecting, transferring, storing and analysing CBU with a high probability of resulting unsuitable for transplantation. PMID:20104277

  1. Application of information technology may increase the efficacy of Confidential Unit Exclusion (CUE) in blood donors

    OpenAIRE

    Jalalian, Mehrdad; Mahboobi, Hamidreza

    2013-01-01

    Confidential Unit Exclusion (CUE) refers to a procedure by which a blood donor designates confidentially whether his or her blood might not be safe for transfusion. It applies to situations in which an individual who is not eligible for blood donation in terms of blood safety feel pressured by others to donate his or her blood. A potential problem in the CUE system is misunderstanding of the procedure and its significance by blood donors as well as the lack of their confidence regarding the c...

  2. Real-Time Blood Donor Management Using Dashboards Based on Data Mining Models

    Directory of Open Access Journals (Sweden)

    Santhanam T

    2011-09-01

    Full Text Available This study uses data mining modeling techniques to examine the blood donor classification and extending this to facilitate the development of realtime blood donor management using dashboards with blood profile and geo-location data. This enables decision makers the ability to manage and plan the blood donation activities based on key metrics. This capability provides the ability to plan effective targeted blood donation campaigns. The scoring algorithm implemented for the dashboard also helps in the optimized deployment of budget resources and budget allocation determination for blood donation campaigns.

  3. Italian National Survey of Blood Donors: External Quality Assessment (EQA) of Syphilis Testing▿

    OpenAIRE

    Vulcano, Francesca; Milazzo, Luisa; Volpi, Sabrina; Battista, Mara Maria; Barca, Alessandra; Hassan, Hamisa Jane; Pimpinelli, Fulvia; Giampaolo, Adele

    2009-01-01

    The detection of syphilis among blood donors may reveal high-risk sexual behavior, which can go unreported at the time of donor selection and compromise the safety of the donated blood. In Italy, blood is collected, tested, and distributed by transfusion services (TSs), which also perform outpatient transfusions. Although the TSs must screen for syphilis by law, there are no indications of the specific type of method to be used, generating discrepancies in the results obtained by the differen...

  4. A STUDY OF DISTRIBUTION OF ABO AND RH BLOOD GROUPS SYSTEM AMONG BLOOD DONORS AT A TERTIARY CARE HOSPITAL

    Directory of Open Access Journals (Sweden)

    Rajesh Kumar

    2015-02-01

    Full Text Available Up till now about 400red cells antigen have been identified. The majority are inherited by Mendelian fashion. The ABO and Rh blood group system was first to be identified and is most important for blood transfusion purposes. OBJECTIVE: This study was conducted to determine the frequency of ABO and Rhesus (Rh blood groups in a tertiary care teaching hospital in India. MATERIALS AND METHODS: A retrospective data based study was conducted at blood bank , Chirayu Medical College and Hospital, Bhopal, Madhya Pradesh, India over a period of four years. RESULTS: Study includes a record of 3188 (28.54% voluntary and 7982 (71.46% replacement donors attending blood bank from February 2011 to January 2015. Out of 11170, 10723(95.998% were male and 447(4.002% female donors. The most common blood group was found to be B in 4013 (35.927% donors followed by O in 3462 (30.994% donors , an in 2516 (22.524% donors and AB in 1179 (10.555% donors. Out of these, 10659(95.425% donors were Rh - positive while 511 ( 4.575 % were Rh - negative.

  5. Frequency and Distribution of Blood Groups in Blood Donors in Western Ahmedabad AND#8211; A Hospital Based Study

    Directory of Open Access Journals (Sweden)

    Patel Piyush A

    2012-04-01

    Full Text Available Background: Up till now about 400 red cells antigen have been identified. The majority are inherited by Mendelian fashion. The ABO and Rhesus (Rh blood group system are most important for blood transfusion purposes, parental testing, legal medicine and in population genetic study. Objective: This study was conducted to determine and compare the frequency of ABO and Rh blood groups in blood donors in secondary care teaching hospital at Western Ahmedabad, Gujarat, India. Materials and Methods: A retrospective study was conducted at Blood bank, GMERS Medical College, Sola, Ahmedabad over a period of seven years from 1st January 2005 to 31st December 2011. Blood group of the blood donors was determined by commercially available standard monoclonal antisera by test tube agglutination technique. Results & conclusion: Out of 5316 subjects, 5076 (95.48% were male and 240 (4.52% were female subjects. The commonest ABO blood group present was B (39.40 % followed by O (30.79 %, A (21.94 % and AB (7.86 % in blood donors; while in Rhesus system, 5053(95.05% donors were Rh-positive and 263(4.95% donors were Rh-negative. The study has a significant implication regarding the inventory management of blood bank and transfusion services for the patient admitted in our secondary care teaching hospital. [National J of Med Res 2012; 2(2.000: 202-206

  6. Risk Factors and Screening for Trypanosoma cruzi Infection of Dutch Blood Donors.

    Directory of Open Access Journals (Sweden)

    Ed Slot

    Full Text Available Blood donors unaware of Trypanosoma cruzi infection may donate infectious blood. Risk factors and the presence of T. cruzi antibodies in at-risk Dutch blood donors were studied to assess whether specific blood safety measures are warranted in the Netherlands.Birth in a country endemic for Chagas disease (CEC, having a mother born in a CEC, or having resided for at least six continuous months in a CEC were considered risk factors for T. cruzi infection. From March through September 2013, risk factor questions were asked to all donors who volunteered to donate blood or blood components. Serum samples were collected from donors reporting one or more risk factors, and screened for IgG antibodies to T. cruzi by EIA.Risk factors for T. cruzi infection were reported by 1,426 of 227,278 donors (0.6%. Testing 1,333 at-risk donors, none (0.0%; 95%, CI 0.0-0.4% was seroreactive for IgG antibodies to T. cruzi. A total of 472 donors were born in a CEC; 553 donors reported their mother being born in a CEC; and 1,121 donors reported a long-term stay in a CEC. The vast majority of reported risk factors were related to Suriname and Brazil. Overall, the participants resided for 7,694 years in CECs, which equals 2.8 million overnight stays. Of those, 1.9 million nights were spent in Suriname.Asymptomatic T. cruzi infection appears to be extremely rare among Dutch blood donors. Blood safety interventions to mitigate the risk of T. cruzi transmission by transfusion would be highly cost-ineffective in the Netherlands, and are thus not required.

  7. Blood Test May Rule Out Too Many Donor Hearts

    Science.gov (United States)

    ... finding ways to sign up more people as organ donors, but there is also a problem in that ... based solely on elevated troponin I if the organ is otherwise suitable. At our institution it has already changed how we evaluate donors, and I think this data will lead to ...

  8. Profile of blood donors with serologic tests reactive for the presence of syphilis in São Paulo, Brazil

    OpenAIRE

    de Almeida Neto, C; Murphy, EL; McFarland, W.; Junior, AM; Chen, S.; Chamone, DA; Sabino, EC

    2009-01-01

    BACKGROUND: Syphilis screening of blood donors is a common practice worldwide, but very little is known about the meaning of a positive serologic test for syphilis in blood donors and the risk profile of these donors. The aim of this study was to determine the demographic characteristics and risk behaviors of blood donors with recent and past syphilis and their implications for blood bank testing and deferral strategies. STUDY DESIGN AND METHODS: Demographic characteristics, category of donat...

  9. Prevalence of Syphilis among Blood and Stem Cell Donors in Saudi Arabia: An Institutional Experience

    Science.gov (United States)

    Elyamany, Ghaleb; Al amro, Mohamed; Pereira, Winston Costa; Alsuhaibani, Omar

    2016-01-01

    Introduction Syphilis is one of the known transfusion-transmissible infections and causes 100,000 deaths yearly, with around 90% of these deaths occurring in the developing world. Little data is available regarding the prevalence of syphilis among Saudi blood and stem cell donors. We conducted a survey on the incidence of syphilis among all blood and stem cell donors. Methods This study was conducted at the Prince Sultan Military Medical City in Riyadh, Saudi Arabia in the 10 years period data during 2006–2015. Data were analyzed about full history, physical examination, age, sex, weight, profession, marital status, number of the donations, data of last donation, having a relation who received blood transfusion, as well as the screening test results of the donated blood. We determined the seroprevalence of infection and compared by sex and other variable through frequency analysis, Chi square, Fisher, and prevalence ratios. Results Approximately 240,000 blood donors were screened and studied in the period of study. Most of the blood donors were male (98.3%) and 89% of them were citizens of Saudi Arabia. According to our findings, we estimated that, in the last 10 years, approximately 0.044% of all the blood donors were syphilis positive cases. No cases were detected as positive for syphilis among stem cell donors. Only 60 blood donors tested positive for syphilis. In addition, we studied 202 stem cell transplant donors during the same period, of which 59% were male and none texted positive for syphilis. Conclusions A concerted effort between the government, health care providers, regulatory bodies and accreditation agencies have all contributed in eliminating the risk of spreading syphilis among blood donors.

  10. Polymerase chain reaction and blood culture in blood donors screened by ELISA test for Chagas' disease

    Directory of Open Access Journals (Sweden)

    Andréa Tieko Kinoshita-Yanaga

    2011-03-01

    Full Text Available The objective of this study was to evaluate, through blood culture and PCR, the results of the ELISA for Chagas' disease in the screening of blood donors in the public blood-supply network of the state of Paraná, Brazil, and to map the epidemiological profile of the donors with respect to their risk of infection by Trypanosoma cruzi. The negative and positive results of the ELISA were confirmed by blood culture and PCR for 190/191 individuals (99.5%. For one individual (0.5%, the ELISA was inconclusive, blood culture and IIF were negative, and IHA and PCR positive. Three individuals (1.6% were positive for T. cruzi on all the tests. Donors were predominantly female, and natives of Paraná, of rural origin, had observed or been informed of the presence of the vector in the municipalities where they resided, had never received a blood transfusion, had donated blood 1 to 4 times, and reported no cases of Chagas' disease in their families. We concluded that PCR and blood culturing have excellent potential for confirming the results of the ELISA, and that candidate blood donors with negative or positive tests have a similar risk of infection by T. cruzi, indicating that the ELISA test is sufficiently safe for screening blood prior to use.O objetivo deste estudo foi avaliar, pela hemocultura e PCR, os resultados do teste ELISA utilizado para doença de Chagas na triagem de doadores de sangue na rede pública do Estado do Paraná, Brasil, e traçar o perfil epidemiológico dos doadores quanto ao risco de infecção pelo Trypanosoma cruzi. Os resultados negativos e positivos do ELISA foram confirmados pela hemocultura e PCR em 190/191 indivíduos (99,5%. Para um indivíduo (0,5%, o teste de ELISA foi inconclusivo, hemocultura e IFI foram negativas, HAI e PCR foram positivas. Três indivíduos (1,6% foram positivos para T. cruzi em todos os testes. A maioria dos doadores era do sexo feminino, oriundos do Estado do Paraná, de origem rural, tinham

  11. A behavior model for blood donors and marketing strategies to retain and attract them

    Directory of Open Access Journals (Sweden)

    Covadonga Aldamiz-echevarria

    2014-06-01

    Full Text Available OBJECTIVE: analyze and propose a theoretical model that describes blood donor decisions to help staff working in blood banks (nurses and others in their efforts to capture and retain donors.METHODS: analysis of several studies on the motivations to give blood in Spain over the last six years, as well as past literature on the topic, the authors' experiences in the last 25 years in over 15 Non Governmental Organizations with different levels of responsibilities, their experiences as blood donors and the informal interviews developed during those 25 years.RESULTS: a model is proposed with different internal and external factors that influence blood donation, as well as the different stages of the decision-making process.CONCLUSION: the knowledge of the donation process permits the development of marketing strategies that help to increase donors and donations.

  12. Risk of cancer after blood transfusion from donors with subclinical cancer: a retrospective cohort study

    DEFF Research Database (Denmark)

    Edgren, Gustaf; Hjalgrim, Henrik; Reilly, Marie;

    2007-01-01

    BACKGROUND: Although mechanisms for detection of short-term complications after blood transfusions are well developed, complications with delayed onset, notably transmission of chronic diseases such as cancer, have been difficult to assess. Our aim was to investigate the possible risk of cancer...... transmission from blood donors to recipients through blood transfusion. METHODS: We did a register-based retrospective cohort study of cancer incidence among patients who received blood from donors deemed to have a subclinical cancer at the time of donation. These precancerous donors were diagnosed......, and essentially complete, population and health-care registers. The risk of cancer in exposed recipients relative to that in recipients who received blood from non-cancerous donors was estimated with multivariate Poisson regression, adjusting for potential confounding factors. FINDINGS: Of the 354 094 transfusion...

  13. Blood donation: comparing individual characteristics, attitudes, and feelings of donors and nondonors.

    Science.gov (United States)

    Griffin, Deborah; Grace, Debra; O'Cass, Aron

    2014-01-01

    The shortage of blood donors and increased demand for blood is an important health issue. Finding ways to increase donor recruitment and retention is a priority and, thus, an important area for research. This article aims to better understand donors and nondonors on the basis of their social responsibility, susceptibility to interpersonal influence, involvement in and attitude towards the blood donation issue, and their aroused feelings. The data from 345 completed surveys were collected via a web-based, self-administered method. Mean differences were examined and the conceptual model was tested via structural equation modeling. The findings provide important clarification of donation and nondonation behavior. PMID:25120042

  14. Prevalence of iron deficiency anaemia among blood donors in Sokoto, North Western, Nigeria

    Directory of Open Access Journals (Sweden)

    Buhari Hauwa Ali

    2015-04-01

    Full Text Available Objective: To investigate the prevalence and socio-demographic factors associated with iron deficiency anaemia among blood donors in Sokoto, North Western, Nigeria using a combination of haemoglobin haematocrit and serum ferritin measurements. Methods: One hundred and fifty consecutively recruited whole blood donors, comprising of 148 (98.7% family replacement donors and 2 (1.3% voluntary non-remunerated donors aged 18-60 years and mean age 39±21 years constituted the subjects for this study. The full blood count was carried out using Mythic 22 CT fully automated haematology analyser (Orphee SA, Switzerland. Serum was tested for ferritin using a human ferritin enzyme immunoassay kitACCU Diag™ ELISA Ferritin kit (Diagnostic Automation/Cortez Diagnostic Inc. California, USA. Results: The prevalence of anaemia (haemoglobin<11.0 g/dL was evident in 24 (16% and iron deficiency anaemia (serum ferritin<12 ng/mL+haemoglobin<11 g/dL in 5 (10% of donors. The haemoglobin and ferritin levels was significantly lower among regular voluntary remunerated blood donors (13.50±0.00 and 34.88±0.00 compared to family replacement donors (14.10±2.40 and 74.12±45.20 respectively (P=0.01 and 0.05 respectively. The mean haemoglobin and ferritin level was compared among donors based on gender. The haemoglobin and ferritin was significantly higher among male donors (14.20±2.00, 78.02±49.10 compared to female donors (12.35±2.5 and 42.20±32.13 (P=0.01. The mean haemoglobin and ferritin level was compared among donors based on occupational groups. The haemoglobin and ferritin was significantly higher among civil servants compared to farmers and students (P=0.01. Conclusions: Iron deficiency anaemia is prevalent among blood donors in Sokoto, North Western, Nigeria. There is need to include routine ferritin in the blood donor testing protocol in the area to enable the diagnosis of donors with latent iron deficiency anaemia to facilitate iron supplementation for

  15. Prevalence of iron deficiency anaemia among blood donors in Sokoto, North Western, Nigeria

    Institute of Scientific and Technical Information of China (English)

    Buhari Hauwa Ali; Yeldu Mohammed Haruna; Erhabor Osaro; Imrana Sani; Abubakar Wase; Onuigwe Festus; Okwesili Augustine; Isaac Zama; Yakubu Abdulrahaman; Dallatu Kabiru

    2015-01-01

    Objective: To investigate the prevalence and socio-demographic factors associated with iron deficiency anaemia among blood donors in Sokoto, North Western, Nigeria using a combination of haemoglobin haematocrit and serum ferritin measurements. Methods: One hundred and fifty consecutively recruited whole blood donors, comprising of 148 (98.7%) family replacement donors and 2 (1.3%) voluntary non-remunerated donors aged 18-60 years and mean age 39±21 years constituted the subjects for this study. The full blood count was carried out using Mythic 22 CT fully automated haematology analyser (Orphee SA, Switzerland). Serum was tested for ferritin using a human ferritin enzyme immunoassay kit-ACCU Diag™ ELISA Ferritin kit (Diagnostic Automation/Cortez Diagnostic Inc. California, USA). Results: The prevalence of anaemia (haemoglobin<11.0 g/dL) was evident in 24 (16%) and iron deficiency anaemia (serum ferritin<12 ng/mL+haemoglobin<11 g/dL) in 5 (10%) of donors. The haemoglobin and ferritin levels was significantly lower among regular voluntary remunerated blood donors (13.50±0.00 and 34.88±0.00) compared to family replacement donors (14.10±2.40 and 74.12±45.20) respectively (P=0.01 and 0.05 respectively). The mean haemoglobin and ferritin level was compared among donors based on gender. The haemoglobin and ferritin was significantly higher among male donors (14.20±2.00, 78.02±49.10) compared to female donors (12.35±2.5 and 42.20±32.13) (P=0.01). The mean haemoglobin and ferritin level was compared among donors based on occupational groups. The haemoglobin and ferritin was significantly higher among civil servants compared to farmers and students (P=0.01). Conclusions: Iron deficiency anaemia is prevalent among blood donors in Sokoto, North Western, Nigeria. There is need to include routine ferritin in the blood donor testing protocol in the area to enable the diagnosis of donors with latent iron deficiency anaemia to facilitate iron supplementation for regular

  16. Influence of postmortem time on the outcome of blood cultures among cadaveric tissue donors.

    Science.gov (United States)

    Saegeman, V; Verhaegen, J; Lismont, D; Verduyckt, B; De Rijdt, T; Ectors, N

    2009-02-01

    Tissue banks provide tissues of human cadaver donors for transplantation. The maximal time limit for tissue retrieval has been set at 24 h postmortem. This study aimed at evaluating the evidence for this limit from a microbiological point of view. The delay of growth in postmortem blood cultures, the identification of the species isolated and clinical/environmental factors were investigated among 100 potential tissue donors. No significant difference was found in the rate of donors with grown blood cultures within (25/65=38%) compared with after (24/65=37%) 24 h of death. Coagulase-negative staphylococci and gastro-intestinal microorganisms were isolated within and after 24 h of death. Two factors--antimicrobial therapy and "delay before body cooling"--were significantly inversely related with donors' blood culture results. From a microbiological point of view, there is no evidence for avoiding tissue retrieval among donors after 24 h of death.

  17. [Automated serial diagnosis of donor blood samples. Ergonomic and economic organization structure].

    Science.gov (United States)

    Stoll, T; Fischer-Fröhlich, C L; Mayer, G; Hanfland, P

    1990-01-01

    A comprehensive computer-aided administration-system for blood-donors is presented. Ciphered informations of barcode-labels allow the automatic and nevertheless selective pipetting of samples by pipetting-robots. Self-acting analysis-results are transferred to a host-computer in order to actualize a donor data-base.

  18. Prevalence of hepatitis B & hepatitis C virus infections in potential blood donors in rural Vietnam

    OpenAIRE

    Viet, Le; Le, Van Viet; Nguyen, Thi Ngoc Lan; Ty, Phung Xuan; Bjørkvoll, Bjørn; Hoel, Hedda B; Gutteberg, Tore Jarl; Husebekk, Anne; Larsen,Stig; Skjerve, Eystein; Husum, Hans

    2012-01-01

    Background & objectives: Safe blood and blood products should be offered to all patients in need for blood transfusion. The objectives of the present study were to establish prevalence estimates for hepatitis B and hepatitis C virus infections as a foundation for safe blood transfusion in rural Vietnam, and to check the accuracy of the laboratory analysis used for hepatitis testing of blood donors in Vietnam. Methods: A cross-sectional study was conducted in two rural communities in Quang Tri...

  19. Plasma-collection plant has to overcome tainted-blood fallout in search for donors

    OpenAIRE

    OReilly, M

    1998-01-01

    Canada's lack of self-sufficiency in blood products has led to the opening of a blood-plasma collection centre in Thunder Bay, Ont.--the first of its type in Canada. In convincing donors to donate plasma, the new centre had to overcome some lingering public concern about the safety of the blood-collection system.

  20. Seroprevalence of anti-HCV antibodies among blood donors of north India

    Directory of Open Access Journals (Sweden)

    R N Makroo

    2013-01-01

    Full Text Available Background & objectives: Transfusion of blood and blood products although considered as a life saving treatment modality, but may lead to certain infectious and non-infectious complications in the recipients. The purpose of this analysis was to monitor the seroprevalence of anti-HCV antibody in the blood donor population in a hospital based blood bank in north India, to evaluate the trends over the years (2001-2011. Methods: Relevant information of all the blood donors who donated whole blood at the department of Transfusion Medicine, Indraprastha Apollo Hospitals, New Delhi from the January 1, 2001 to December 31, 2011 was retrieved from the departmental records. The number of donors who were found reactive for anti-HCV anatibodies was calculated. Results: Of the 2,06,022 blood donors, 1,93,661 were males and 12,361 were females. The percentage of whole blood donors found seroreactive for anti-HCV antibodies was 0.39 per cent (n=795. The seroprevalence of anti-HCV in male blood donors was 0.38 per cent (n=750 and the respective seroprevalence in female blood donors was 0.36 per cent (n=45. No significant change in the trend of HCV seroprevalence was observed over the period under consideration. Maximum seroprevalence of anti-HCV was observed in the age group of 18 to 30 yr (0.41% and the minimum in the age group of 51 to 60 yr (0.26%. Interpretation & conclusion: HCV seroprevalence in our study was 0.39 per cent and a decreasing trend with age was observed. No significant change in the trend of anti-HCV seroprevalence was seen over a decade. Since, no vaccine is presently available for immunization against HCV infection, transfusion transmitted HCV infection remains a potential threat to the safety of the blood supply.

  1. Red cell antigen prevalence predicted by molecular testing in ethnic groups of South Texas blood donors.

    Science.gov (United States)

    Aranda, Lorena I; Smith, Linda A; Jones, Scott; Beddard, Rachel

    2015-01-01

    Alloimmunization to red blood cell antigens is seen in patients receiving chronic blood transfusion. Knowing the prevalence of blood group antigens of the different ethnicities of South Texas donors can provide better management of rare blood inventory for patients in this geographical area. A total of 4369 blood donors were tested and analyzed for various antigens in the following blood group systems: ABO, Rh, Kell, Duffy, Kidd, MNS, Lutheran, Dombrock, Landsteiner-Wiener, Diego, Colton, and Scianna. Donors tested to be group 0 or A were serologically tested for the Rh (C, E, c, e) antigens. Those that tested as presumably R1R1, R2R2, or Ror were then genotyped. Donors constituted three major ethnicities: black (18.3%), Hispanic (36.3%), and Caucasian (41.1%); ethnicities comprised of Asian, American Indian, multiracial, and other accounted for the remaining donors (4.3%). The most likely common Rh phenotype for each ethnicity is as follows: black -Ror (44.4%), Hispanic -R1R1 (59.0%), and Caucasian -R1R1 (38.9%). The prevalence of Kell, Duffy, and Kidd blood group system antigens in black and Caucasian donors is comparable with published reports for the entire U.S. The black South Texas donor population had an 8.8 percent increase in prevalence of the Fy(a+b-) phenotype as compared with these published reports; the Hispanic South Texas donor population had a prevalence of 36.1 percent of the Fy(a+b-) phenotype. Regarding the Diego blood group system, the Hispanic donor population in South Texas had a prevalence of 93.5 percent for the Di(a-b+) phenotype as compared with published reports for the entire U.S. (>99.9%). The Hispanic population had a prevalence of 7.9 percent of donors testing as M-N+S-s+ as compared with 20.2 percent and 15.6 percent for black and Caucasian donors, respectively. This study helped us determine the prevalence of each of the blood group antigens in the South Texas donor population to establish and maintain adequate rare inventory of

  2. Red cell antigen prevalence predicted by molecular testing in ethnic groups of South Texas blood donors.

    Science.gov (United States)

    Aranda, Lorena I; Smith, Linda A; Jones, Scott; Beddard, Rachel

    2015-01-01

    Alloimmunization to red blood cell antigens is seen in patients receiving chronic blood transfusion. Knowing the prevalence of blood group antigens of the different ethnicities of South Texas donors can provide better management of rare blood inventory for patients in this geographical area. A total of 4369 blood donors were tested and analyzed for various antigens in the following blood group systems: ABO, Rh, Kell, Duffy, Kidd, MNS, Lutheran, Dombrock, Landsteiner-Wiener, Diego, Colton, and Scianna. Donors tested to be group 0 or A were serologically tested for the Rh (C, E, c, e) antigens. Those that tested as presumably R1R1, R2R2, or Ror were then genotyped. Donors constituted three major ethnicities: black (18.3%), Hispanic (36.3%), and Caucasian (41.1%); ethnicities comprised of Asian, American Indian, multiracial, and other accounted for the remaining donors (4.3%). The most likely common Rh phenotype for each ethnicity is as follows: black -Ror (44.4%), Hispanic -R1R1 (59.0%), and Caucasian -R1R1 (38.9%). The prevalence of Kell, Duffy, and Kidd blood group system antigens in black and Caucasian donors is comparable with published reports for the entire U.S. The black South Texas donor population had an 8.8 percent increase in prevalence of the Fy(a+b-) phenotype as compared with these published reports; the Hispanic South Texas donor population had a prevalence of 36.1 percent of the Fy(a+b-) phenotype. Regarding the Diego blood group system, the Hispanic donor population in South Texas had a prevalence of 93.5 percent for the Di(a-b+) phenotype as compared with published reports for the entire U.S. (>99.9%). The Hispanic population had a prevalence of 7.9 percent of donors testing as M-N+S-s+ as compared with 20.2 percent and 15.6 percent for black and Caucasian donors, respectively. This study helped us determine the prevalence of each of the blood group antigens in the South Texas donor population to establish and maintain adequate rare inventory of

  3. Sero-prevalence of Human Cytomegalovirus among blood donors in Lahore, Pakistan

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    Chahat Batool Rizvi

    2015-08-01

    Full Text Available Background: Transfusion-transmitted cytomegalovirus (TT-CMV infection can cause severe illness and even death among immunocompromised patients; therefore, the spread of CMV through blood products should be prevented. To our knowledge, no study has been carried out in Pakistan to determine the seroprevalence of CMV in general population as well as among blood donors. The goal of this study was to determine CMV seropositivity among blood donors at the blood bank of INMOL Hospital, Lahore, Pakistan. Methods: A sero-epidemiological cross-sectional study was conducted. Sera from 91 blood donors were screened for CMV specific IgG antibodies by enzyme-linked immunosorbent assay (ELISA based kit. Results: The CMV-specific IgG antibodies were detected in 89 blood donors, which gave seroprevalence rate of 97.8%. The statistical analysis of results was done using pearson chi-square test and appeared non-significant with values 0.625 and 0.705 for different age groups and blood groups of donors. Conclusion: Because of high seroprevalence in this study area, an adequate supply of CMV seronegative blood is difficult to maintain. Therefore, we propose that the future strategies for the prevention of post-transfusion CMV infection in recipients should include the transfusion of leukoreduced blood products. Further a prospective study with much greater population can be done to identify major causative risk factors for such highest prevalence rate.

  4. Hepatitis B Prevalence and Risk Factors in Blood Donors in Ghazvin, IR.Iran|

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    Babak Yektaparast

    2005-12-01

    Full Text Available Background and Aims: This study was done to find out the common routes of transmission of hepatitis B.Methods: We assessed 39598 volunteer blood donors for hepatitis B and C. Risk factors were obtained from 186 patients and 186 healthy donors. Independent risk factors were determined using logistic regression analysis.Results: Prevalence of HBV was 1.08%. Female sex, education level lower than secondary school, being married, and age more than 35 years old, were risk factors in univariate analysis. Logistic regression showed that only duration of marriage, close contact with an HBV infected person, extramarital sexual contact, history of sexually transmitted diseases and high risk jobs were independent risk factors for prediction of hepatitis B infection. Risk factors which were addressed in this study covered 95.7% of the patients. Conclusions: Ghazvin is one of the low prevalent regions for hepatitis B in Iran. Prevalence of hepatitis B is decreasing in comparison with past decades. Horizontal mode is more important than vertical transmission in this region of Iran.Screening programs, education and vaccination, specifically in high risk groups are essential for prevention of new cases

  5. Prevalence of undiagnosed diabetes and prediabetes among voluntary blood donors in a tertiary health care setting

    OpenAIRE

    Jawahar Ramasamy; Umadevi Komarla Parthasarathy

    2016-01-01

    Background: Finnish diabetes risk score (FINDRISC) questionnaire is a screening tool to estimate risk of type 2 diabetes. This study was conducted to find the prevalence rate of diabetes and prediabetes among voluntary blood donors using FINDRISC score as screening tool in large population. Methods: A total of 210 eligible blood donors were included in this study. After obtaining the consent, subjects will be assessed with FINDRISC questionnaire. The patients with score and #8805;12 poin...

  6. [Correlation between Staphylococcus carriage, specific antibody-production and AB0-blood grouping in plasma donors].

    Science.gov (United States)

    Nemyrovs'ka, L M; Patoka, V V

    2002-01-01

    Interaction peculiarities of three components of the immune human homeostasis-antigens of blood groups AB0, staphylococcus antigens and antistaphylococcus antibodies have been investigated. Donors (85) of antistaphylococcus plasma immunized by staphylococcus anatoxin have been investigated. It is found that the nasal staphylococcus carriage in donors depends on the level of specific and natural antibodies and on the coincidence between the staphylococcus antigen structure and the protein substance of the specific blood group factors. PMID:12190026

  7. Is current serologic RhD typing of blood donors sufficient for avoiding immunization of recipients?

    DEFF Research Database (Denmark)

    Krog, Grethe Risum; Clausen, Frederik Banch; Berkowicz, Adela;

    2011-01-01

    Avoiding immunization with clinically important antibodies is a primary objective in transfusion medicine. Therefore, it is central to identify the extent of D antigens that escape routine RhD typing of blood donors and to improve methodology if necessary.......Avoiding immunization with clinically important antibodies is a primary objective in transfusion medicine. Therefore, it is central to identify the extent of D antigens that escape routine RhD typing of blood donors and to improve methodology if necessary....

  8. Effectiveness of DNA-recombinant anti-hepatitis B vaccines in blood donors: a cohort study

    OpenAIRE

    Petry Andrea; de Souza Denise ER; Kupek Emil

    2007-01-01

    Abstract Background Although various studies have demonstrated efficacy of DNA-recombinant anti-hepatitis B vaccines, their effectiveness in health care settings has not been researched adequately. This gap is particularly visible for blood donors, a group of significant importance in the reduction of transfusion-transmitted hepatitis B. Methods This is a double cohort study of 1411 repeat blood donors during the period 1998–2002, involving a vaccinated and an unvaccinated cohort, with matchi...

  9. Significance of isolated hepatitis B core antibody in blood donors from São Paulo

    OpenAIRE

    Cesar de ALMEIDA NETO; Strauss, Edna; SABINO Esther Cerdeira; Sucupira, Maria Cecília Araripe; CHAMONE Dalton de Alencar Fischer

    2001-01-01

    The clinical significance of isolated anti-HBc is still a challenge. To elucidate the real importance of this finding in our blood donors, an investigation algorithm was tested. One hundred and twelve isolated anti-HBc seropositive blood donors underwent clinical evaluation and retesting of HBV markers. Those who presented repeatedly reactive isolated anti-HBc, received a single dose of hepatitis B recombinant vaccine to verify anti-HBs early response. A HBV-DNA determination by PCR was done ...

  10. Spillovers of Prosocial Motivation: Evidence from an Intervention Study on Blood Donors

    OpenAIRE

    Bruhin, Adrian; Götte, Lorenz; Haenni, Simon; Jiang, Lingqing

    2014-01-01

    Spillovers of prosocial motivation are crucial for the formation of social capital. They facilitate interactions among individuals and create social multipliers that amplify the effects of policy interventions. We conducted a large-scale intervention study among dyads of blood donors to investigate whether social ties lead to motivational spillovers in the decision to donate. The intervention is a randomized phone call making donors aware of a current shortage of their blood type and serving ...

  11. Frequency of thalassemia carrier and Hb variant and the quality of stored donor blood

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    Eva A. Maharani

    2015-01-01

    Full Text Available Background: This study was aimed to determine the frequency of thalassemia and Hb variant in blood donor. In addition, we also wanted to know the quality of blood from the donor up to seven days of storage, by calculating percentage of hemolysis in vitro.Methods: This cross-sectional study was conducted on 138 blood donor specimens at Red Cross Blood Centre Unit in Jakarta. All specimens were tested for thalassemia and Hb variant by complete blood count (CBC and Hb analysis with HPLC method and DNA analysis for the detection of α thalassemia carrier. To analyze the quality of stored blood, the calculation of hemolytic rate of red blood cells (RBCs on whole blood (WB was compared between the first and seventh days of storage.Results: Out of the 138 specimens, 5 samples (3.6% were diagnosed for α thalassemia carrier in which, one of them is co-inherited with ovalositosis hereditary (Southeast Asian Ovalositosis/SAO, 3 samples (2.2% for β thalassemia carrier, and 3 samples (2.2% for Hb E. Meanwhile, the hemolytic rates of RBCs on WB in first day and seven day of storage were below one percent.Conclusion: The frequency of thalassemia carrier and Hb variants in blood donors at Red Cross Blood Centre Unit in Jakarta was 8%. The quality of stored blood until seven day of storage was quite good.

  12. Assessing Acceptability of Short Message Service Based Interventions towards Becoming Future Voluntary Blood Donors

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    Sana Saleem

    2014-01-01

    Full Text Available All blood bank services, especially those of developing countries, face a major shortfall of blood donations due to lack of voluntary blood donors. Our study aims to evaluate the acceptability of Short Message Service based interventions towards becoming voluntary blood donors among medical university students of Karachi, Pakistan. Methods. A total of 350 medical students were approached in medical universities of Karachi, Pakistan, using a nonprobability convenient sampling technique. Data collectors administered a self-made questionnaire to each participant using an interview based format. All data was recorded and analyzed on SPSS 16. Results. 350 participants, having a mean age of 21.47 ± 1.36, were included in our study with 30.6% (107/350 being males and 69.4% (243/350 being females. 93.4% (327/350 of participants agreed that donating blood was healthy, but only 26% had donated blood in the past with 79.1% donating voluntarily. 65.7% (230/350 of the participants agreed to take part in Short Message Service based behavioral interventions to become voluntary blood donors with 69.7% (244/350 also agreeing that Short Message Service reminders will promote them to donate blood more often. Conclusion. With university students willing to become voluntary blood donors, Pakistani blood banks can carry out Short Message Service based interventions to encourage them to donate blood.

  13. Viral metagenomics applied to blood donors and recipients at high risk for blood-borne infections

    Science.gov (United States)

    Sauvage, Virginie; Laperche, Syria; Cheval, Justine; Muth, Erika; Dubois, Myriam; Boizeau, Laure; Hébert, Charles; Lionnet, François; Lefrère, Jean-Jacques; Eloit, Marc

    2016-01-01

    Background Characterisation of human-associated viral communities is essential for epidemiological surveillance and to be able to anticipate new potential threats for blood transfusion safety. In high-resource countries, the risk of blood-borne agent transmission of well-known viruses (HBV, HCV, HIV and HTLV) is currently considered to be under control. However, other unknown or unsuspected viruses may be transmitted to recipients by blood-derived products. To investigate this, the virome of plasma from individuals at high risk for parenterally and sexually transmitted infections was analysed by high throughput sequencing (HTS). Materials and methods Purified nucleic acids from two pools of 50 samples from recipients of multiple transfusions, and three pools containing seven plasma samples from either HBV−, HCV− or HIV-infected blood donors, were submitted to HTS. Results Sequences from resident anelloviruses and HPgV were evidenced in all pools. HBV and HCV sequences were detected in pools containing 3.8×103 IU/mL of HBV-DNA and 1.7×105 IU/mL of HCV-RNA, respectively, whereas no HIV sequence was found in a pool of 150 copies/mL of HIV-RNA. This suggests a lack of sensitivity in HTS performance in detecting low levels of virus. In addition, this study identified other issues, including laboratory contaminants and the uncertainty of taxonomic assignment of short sequence. No sequence suggestive of a new viral species was identified. Discussion This study did not identify any new blood-borne virus in high-risk individuals. However, rare and/or viruses present at very low titre could have escaped our protocol. Our results demonstrate the positive contribution of HTS in the detection of viral sequences in blood donations. PMID:27136432

  14. West Nile virus lineage 2 infection in a blood donor from Vienna, Austria, August 2014.

    Science.gov (United States)

    Jungbauer, C; Hourfar, M K; Stiasny, K; Aberle, S W; Cadar, D; Schmidt-Chanasit, J; Mayr, W R

    2015-03-01

    Eastern Austria is neighbouring regions with ongoing West Nile virus (WNV) transmissions. Three human WNV infections had been diagnosed during the past decade in Austria. The Austrian Red Cross Blood Service (ARC-BS) started a first voluntary screening for WNV in blood donors from Eastern Austria by Nucleic Acid Testing (NAT) in June 2014. This is also the most extensive WNV surveillance programme in humans in Austria so far. In August 2014, one autochthonous WNV infection was detected in a blood donor from Vienna. By now, one in 67,800 whole blood donations was found to be positive for WNV RNA.

  15. Mechanism of altruism approach to blood donor recruitment and retention: a review and future directions.

    Science.gov (United States)

    Ferguson, E

    2015-08-01

    Why do people donate blood? Altruism is the common answer. However, altruism is a complex construct and to answer this question requires a systematic analysis of the insights from the biology, economics and psychology of altruism. I term this the mechanism of altruism (MOA) approach and apply it here for understanding blood donor motivation. The answer also has enormous implications for the type of interventions we choose to adopt as a society. A MOA approach so far shows that blood donors are a mixture of (i) warm-glow givers (donation is emotionally rewarding) and (ii) reluctant altruists (cooperate rather than defect when free-riding is high). Donors also show 'saintly sinning' with the extra 'moral currency' form blood donation allowing them to be less generous in other contexts. The MOA approach suggests why financial incentives, in terms of gifts/lottery tickets, are effective and suggests a number of novel interventions for donor recruitment: 'voluntary reciprocal altruism' and 'charitable incentivisation'. The MOA approach also highlights the need for an intervention developed specifically for recipients to allow them to show their gratitude to donors and for society to celebrate blood donation. It is suggests a 'Monument to Blood Donors' will achieve this. The approach suggests a number of novel research questions into (i) donor self-selection effects, (ii) conditional cooperation and (iii) construct overlap with Theory of Planned Behaviour (e.g. affective attitudes and warm-glow). The MOA offers a powerful way to understand blood donor motivations around altruism and develop theoretically driven interventions. PMID:26311129

  16. Prevalence of glucose-6-phosphate dehydrogenase deficiency and sickle cell trait among blood donors in Riyadh

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    Alabdulaali Mohammed

    2010-01-01

    Full Text Available Background and Aims: Blood donation from glucose-6-phosphate dehydrogenase (G6PD-deficient and sickle cell trait (SCT donors might alter the quality of the donated blood during processing, storage or in the recipient′s circulatory system. The aim of this study was to determine the prevalence of G6PD deficiency and SCT among blood donors coming to King Khalid University Hospital (KKUH in Riyadh. It was also reviewed the benefits and risks of transfusing blood from these blood donors. Materials and Methods: This cross-sectional study was conducted on 1150 blood samples obtained from blood donors that presented to KKUH blood bank during the period April 2006 to May 2006. All samples were tested for Hb-S by solubility test, alkaline gel electrophoresis; and for G6PD deficiency, by fluorescent spot test. Results: Out of the 1150 donors, 23 (2% were diagnosed for SCT, 9 (0.78% for G6PD deficiency and 4 (0.35% for both conditions. Our prevalence of SCT and G6PD deficiency is higher than that of the general population of Riyadh. Conclusion: We recommend to screen all units for G6PD deficiency and sickle cell trait and to defer donations from donors with either of these conditions, unless if needed for special blood group compatibility, platelet apheresis or if these are likely to affect the blood bank inventory. If such blood is to be used, special precautions need to be undertaken to avoid complications in high-risk recipients.

  17. Measures to decrease the risk of acquired immunodeficiency syndrome transmission by blood transfusion. Evidence of volunteer blood donor cooperation.

    Science.gov (United States)

    Pindyck, J; Waldman, A; Zang, E; Oleszko, W; Lowy, M; Bianco, C

    1985-01-01

    We studied whether volunteers giving blood to the Greater New York Blood Program (GNYBP) cooperated with procedures implementing public health recommendations intended to decrease the risk of acquired immunodeficiency syndrome (AIDS) transmission by blood transfusion. Predonation medical screening was expanded to exclude donors who might be ill with AIDS. To exclude possible asymptomatic carriers of the disease, members of groups at increased risk of AIDS were asked either not to give blood or to give it for laboratory studies. A confidential questionnaire, administered to all donors after medical screening, provided the vehicle for donors to advise the GNYBP whether their donation was for laboratory studies or for patient transfusion. We found that the number of male donors decreased; AIDS-related questions in medical history led to a 2 percent increase in donor rejections; 97 percent of donors said their blood could be used for transfusions; 1.4 percent said their blood could be used for laboratory studies only; and 1.6 percent did not respond. Only units designated for transfusion were released to hospitals. People who indicated that their donation was for laboratory studies had a higher prevalence of markers for hepatitis B virus and of antibodies to cytomegalovirus. White cell counts and helper/suppressor T lymphocyte ratios were not significantly different in the two groups. We conclude that volunteer donors have cooperated with the established procedures. None of the laboratory assays identified blood units donated by individuals who, based on information about AIDS high-risk groups, designated their donation for laboratory studies. PMID:3969698

  18. Risk factors for hepatitis B carrier status among blood donors of the National Blood Center, Thai Red Cross Society.

    Science.gov (United States)

    Nuchprayoon, T; Chumnijarakij, T

    1992-06-01

    A study of risk factors for hepatitis B carriers among voluntary blood donors of the National Blood Center, Thai Red Cross Society was carried out in a case-control study design during January 1989 to June 1990. Cases were 876 blood donors whose blood identified HBsAg at time of recruitment and continued positive for more than 6 months. Controls were 1,750 blood donors whose blood was free from HBsAg who came for blood donation at the same period as the cases. The ratio of cases:controls = 1:2. Self-administered questionnaires were constructed and pretested before using both cases and controls. The study revealed that the risk factors for hepatitis B among voluntary blood donors were age of less than 30 years old; low socioeconomic status (family income of less than 8,000 Baht/month); single status, especially males; male occupations of students, monks, nongovernment workers compared with government officials; female occupations of laborers, students, nongovernment workers and government officials compared with housewives. Sharing of nail clippers, used blades and tooth brushes among family members are proved to be risk factors, especially among males. In addition, sharing of used blades in barber shops proved to be a risk among males while sharing of nail clippers in beauty salons, history of ear-piercing at department stores or history of caesarean section among females could not be shown to be risk factors.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:1439976

  19. Safe Blood Transfusion: Screening for Hepatitis B and Hepatitis C Virus Infections in Potential Blood Donors in Rural Southeast Asia

    OpenAIRE

    Le, Van Viet

    2014-01-01

    2,400 blood samples from potential voluntary rural blood donors in a multicentre cross-sectional study in Cambodia and Vietnam were analysed with rapid and EIA tests for detection of HBsAg, anti-HBc and anti-HCV at local laboratories. 640 randomly selected blood samples were blindly validated in a Norwegian accredited micro-laboratory by a chemiluminescent micro particle immunoassay technique (CMIA). Rapid test for donor screening of hepatitis proved to have far lover accuracy than claimed by...

  20. Seroprevalence of transfusion transmissible infections among blood donors at the blood bank of a Medical College of Kolkata

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    Prasanta Ray Karmakar

    2014-01-01

    Full Text Available Seroprevalence of transfusion transmissible infections (TTIs among blood donors can be used to monitor the prevalence among apparently healthy adult population. The present study was conducted to determine the profile of blood donors and seroprevalence of TTI among them. Retrospective analysis of the donors of a blood bank attached with a tertiary care hospital of Kolkata in 2011 was carried out. Data were analyzed with SPSS version 17. Majority (85% of the donors were male, two-third in the age group of 21-40 years. Among the donors 2.79% were positive for any of the screened TTIs. Seroprevalence was highest for hepatitis B (1.41% followed by human immunodeficiency virus (0.60% and hepatitis C (0.59% and least for syphilis (0.23%. Seropositivity increased with age up to 50 years. There was no significant difference in seropositivity between male and female. Highly sensitive donor screening and public awareness program can make transfusion of blood products safe.

  1. Seroprevalence of transfusion transmissible infections among blood donors at the blood bank of a Medical College of Kolkata.

    Science.gov (United States)

    Karmakar, Prasanta Ray; Shrivastava, Prabha; Ray, Tapobrata Guha

    2014-01-01

    Seroprevalence of transfusion transmissible infections (TTIs) among blood donors can be used to monitor the prevalence among apparently healthy adult population. The present study was conducted to determine the profile of blood donors and seroprevalence of TTI among them. Retrospective analysis of the donors of a blood bank attached with a tertiary care hospital of Kolkata in 2011 was carried out. Data were analyzed with SPSS version 17. Majority (85%) of the donors were male, two-third in the age group of 21-40 years. Among the donors 2.79% were positive for any of the screened TTIs. Seroprevalence was highest for hepatitis B (1.41%) followed by human immunodeficiency virus (0.60%) and hepatitis C (0.59%) and least for syphilis (0.23%). Seropositivity increased with age up to 50 years. There was no significant difference in seropositivity between male and female. Highly sensitive donor screening and public awareness program can make transfusion of blood products safe.

  2. Hepatitis G Virus Infection in Iranian Blood Donors and High-Risk Groups

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    Shahram Samiei

    2009-11-01

    Full Text Available Background and Aims: Hepatitis G virus (HGV has a worldwide distribution, and the prevalence rates among blood donors and high-risk groups are different. The purpose of this study was to assess the frequency of the HGV infection in blood donors as a blood borne pathogen and in high-risk groups (multitransfused patients, such as thalassemic, hemophillic, and hemodialysis patients.Methods: 400 Iranian (Tehran Blood Transfusion Center, 2004 blood donors were tested for HGV RNA by a reverse transcriptase chain reaction (RT-PCR method. The participants were negative in blood screening tests for hepatitis B surface antigen (HBsAg, hepatitis C virus antibodies (anti- HCV, human immunodeficiency virus (HIV Ag/Ab, and Rapid Plasma Reagin (RPR. HGV RNA positivity was surveyed in 40 thalassaemic, 16 hemophilic, and 46 hemodialysis patients by RT-PCR. To assess the frequency of infection, the prevalence of HGV RNA positive cases per 100 donors/patients with 95% confidence intervals (95% CI were calculated. P values were estimated with chi-square tests.Results: 19 (4.8%; 95% CI: 2.9-6.5% out of 400 blood donors samples were HGV RNA positive. The prevalence of HGV infection was 5.28% (13 out of 243 in repeat donors, 4.12% (4 in 99 in lapsed donors, and 3.50% (2 out of 58 in first-time blood donors. The combined prevalence of HGV infection in these groups of patients was 16 (15.7%; 95% CI: 8.3-23.1% out of 102 samples. HGV RNA frequency was 1 out of 40 (2.5%; 95% CI: 1.8-3.2% thalassemic patients, 15 out of 46 (32.6%; 95% CI: 16.8-48.4% hemodialysis patients, and 0 out of 16 hemophilics patients.Conclusions: The prevalence of HGV RNA in the high-risk population was 15.7% and nearly 3 times more than blood donors (4.8%. These data indicate the possibility of parenteral transmission of HGV, especially by transfusion of blood and blood components. Decisions to screen blood supplies for a transfusion-transmitted infection agent should be based on sufficient

  3. Screening of β-thalassemia trait and other hemoglobinopathies among blood donors in Punjab

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    Rajesh Kumar

    2015-01-01

    Full Text Available Introduction: Hemoglobinopathies are common genetic disorders of hemoglobin, which can be prevented by population screening and offering genetic counseling. The cumulative gene frequency of hemoglobinopathies in India is 4.2%. The carrier state for β-thalassemia in India varies from 1% to 17% with an average of 3.2%. The present study was undertaken to find out the burden of hemoglobinopathies and spectrum of this disorders among the blood donors. Materials and Methods: The study includes 975 students between 18 and 25 years who donated blood, were screened for β-thalassemia trait and related hemoglobinopathies by high-performance liquid chromatography (HPLC using Bio-Rad variant. Samples were also run on a hemato analyzer for red cell indices and peripheral smear for red cell morphology. Results: A total of 41 donors showed abnormal hemoglobin fractions in HPLC. Out of these, 32 (3.3% were diagnosed with β-thalassemia trait, 8 (0.8% with Hb-D Punjab and 1 (0.1% with Hb-S trait. The frequency of β-thalassemia trait in the student from different geographical regions varied from 0.8% to 4.44%, being the highest in those from Punjab and frequency of β-thalassemia trait in different caste groups varied from 0 to 4.74%, being the highest in the student from Jatt Sikh community. Conclusions: A universal approach of screening for β-thalassemia trait should be included as a part of standard blood testing among the college students, premarital and of the extended family of thalassemics. Population group with high gene frequencies requires screening programs as well as increased awareness and education program to control the birth of thalassemia major.

  4. Increased mortality associated with HTLV-II infection in blood donors: a prospective cohort study

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    Smith James W

    2004-03-01

    Full Text Available Abstract Background HTLV-I is associated with adult T-cell leukemia, and both HTLV-I and -II are associated with HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP. Several published reports suggest that HTLV-I may lead to decreased survival, but HTLV-II has not previously been associated with mortality. Results We examined deaths among 138 HTLV-I, 358 HTLV-II, and 759 uninfected controls enrolled in a prospective cohort study of U.S. blood donors followed biannually since 1992. Proportional hazards models yielded hazard ratios (HRs for the association between mortality and HTLV infection, controlling for sex, race/ethnicity, age, income, educational level, blood center, smoking, injection drug use history, alcohol intake, hepatitis C status and autologous donation. After a median follow-up of 8.6 years, there were 45 confirmed subject deaths. HTLV-I infection did not convey a statistically significant excess risk of mortality (unadjusted HR 1.9, 95%CI 0.8–4.4; adjusted HR 1.9, 95%CI 0.8–4.6. HTLV-II was associated with death in both the unadjusted model (HR 2.8, 95%CI 1.5–5.5 and in the adjusted model (HR 2.3, 95%CI 1.1–4.9. No single cause of death appeared responsible for the HTLV-II effect. Conclusions After adjusting for known and potential confounders, HTLV-II infection is associated with increased mortality among healthy blood donors. If replicated in other cohorts, this finding has implications for both HTLV pathogenesis and counseling of infected persons.

  5. The use of vascular access ports for blood collection in feline blood donors.

    Science.gov (United States)

    Aubert, Isabelle; Abrams-Ogg, Anthony C G; Sylvestre, Anne M; Dyson, Doris H; Allen, Dana G; Johnstone, Ian B

    2011-01-01

    We investigated vascular access ports for feline blood donation. Eight cats were anesthetized for conventional blood collection by jugular venipuncture at the beginning and end of the study. In-between conventional collections, vascular access ports were used for collection with or without sedation every 6 to 8 wk for 6 mo. Ports remained functional except for one catheter breakage, but intermittent occlusions occurred. Systolic blood pressure was lower during conventional collection. Behavioral abnormalities occurred during 3 port collections. Packed red cells prepared from collected blood were stored at 4°C for 25 d and assessed for quality pre- and post-storage. With both collection methods, pH and glucose level declined, and potassium level, lactate dehydrogenase activity and osmotic fragility increased. There were no differences between methods in pre-storage albumin and HCO(3)(-) levels, and pre and post-storage hematocrit, lactate dehydrogenase activity, and glucose and potassium levels. Pre-storage pH and pCO(2) were higher with conventional collection, and pre- and post-storage osmotic fragility were greater with port collection. One port became infected, but all cultures of packed red cells were negative. Tissue inflammation was evident at port removal. In a second study of conventional collection in 6 cats, use of acepromazine in premedication did not exacerbate hypotension. The use of vascular access ports for feline blood donation is feasible, is associated with less hypotension, and may simplify donation, but red cell quality may decrease, and effects on donors must be considered. PMID:21461192

  6. Comparison of Stored Umbilical Cord Blood and Adult Donor Blood: Transfusion Feasibility

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    Rola Sahyoun-tokan

    2012-09-01

    Full Text Available OBJECTIVE: This study aimed to compare the storage properties of red blood cell (RBC concentrates of umbilical cord blood (UCB and adult donor blood (ADB, and to evaluate the feasibility of UCB-RBC concentrate as an autologous source for blood transfusion in very low birth weight (VLBW preterm neonates. METHODS: In all, 30 newborn (10 preterm, 20 full term UCB and 31 ADB units were collected. RBC concentrates were stored and compared with regard to pH, potassium (K+, 2,3-biphosphoglycerate (2-3-BPG, adenosine tri-phosphate (ATP, plasma Hb, and bacterial contamination on d 1, 21, and 35 of storage. RESULTS: The K+ level increased with time and differed significantly between storage d 1 and 21, and between storage d 1 and 35 in both the UCB and ADB units. Initial and d 21 K+ levels were higher in the UCB units than in the ADB units. The 2,3-BPG level did not differ significantly between the UCB-PRC and ADB-PRC samples. After 35 d of storage both UCB-PRC and ADB-PRC samples exhibited significant differences from the initial free Hb, intracellular ATP, and pH values. Significant differences in intracellular ATP and pH were also observed between the UCB-PRC and ADB-PRC samples. CONCLUSION: The volume of harvested and prepared UCB-PRC can be used for some of the blood transfusions required during the neonatal period and thus may decrease the number of allogeneic transfusions, especially in preterm newborns. The hematological and biochemical changes that occurred in UCB during storage were comparable with those observed in ADB, and do not pose a risk to the immature metabolism of neonates. UCB-RPC prepared and stored under standard conditions can be a safe alternative RBC source for transfusions in VLBW newborns.

  7. Prevalence and Trends of Transfusion-Transmissible Viral Infections among Blood Donors in South of Iran: An Eleven-Year Retrospective Study

    Science.gov (United States)

    Farshadpour, Fatemeh; Taherkhani, Reza; Tajbakhsh, Saeed; Gholizadeh Tangestani, Marziyeh; Hajiani, Gholamreza; Sharifi, Nasrin; Taherkhani, Sakineh; Nejadbolkheyr, Abdolreza

    2016-01-01

    Background Blood transfusion is considered a potential risk factor for transmission of life-threatening viral infections, including HIV, HCV and HBV infections. This study was performed to find out the prevalence and trends of these infections among blood donors in Southern Iran. Methods The blood donor data recorded in twelve regional blood transfusion centers from 2004 to 2014 were analyzed in an anonymous way with respect to the results of serological screening for HBV, HCV, and HIV infections. Overall, 293454 donors were screened for viral infections. Results Most of the donors were male, married, aged between 20–40 years, educated, and regular donors. The overall seroprevalence rates of HBV, HCV and HIV were 0.15%, 0.1% and 0.004%, respectively. The highest seroprevalence was found for HBV, followed by HCV and HIV. These infections were more prevalent in male, low educated and first time donors. The highest HCV seroprevalence was observed among donors aged 20 to 40 years, while HBV seroprevalence increased with age. The seroprevalence rates of HBV and HCV from 2004 to 2014 showed significant decreasing trends from 0.460% to 0.060% (P < 0.001) and 0.329% to 0.045% (P < 0.001), respectively. Whereas HIV infection had a slight but not significant decline from 0.0173% in 2004 to 0.0028% in 2014 (P = 0.087). Conclusions The decreasing trends of transfusion-transmissible viral infections in blood donations indicate that the attempts of IBTO were successful in improving the safety of the blood supply, since the prevalence rates of viral infections have been reduced to very low levels in blood donations over the years. However, still more effective techniques such as polymerase chain reaction (PCR) are needed to guarantee blood safety. PMID:27309959

  8. Sex, risk, and education in donor educational materials: review and critique.

    Science.gov (United States)

    Cascio, M Ariel; Yomtovian, Roslyn

    2013-01-01

    Food and Drug Administration guidelines prohibit men who have sex with men (MSM) from donating blood to prevent the spread of the human immunodeficiency virus (HIV/AIDS). Although the deferral criteria leave "sex" undefined, donor educational materials distributed before the health questionnaire often offer a definition. This study analyzes educational materials for their contribution to the donation process and construction of HIV/AIDS. It applies a discourse analysis approach to a sample (n = 52) of such materials obtained in summer 2009 from blood collection organizations listed in the AABB (now referred to as "Advancing Transfusion and Cellular Therapies Worldwide") Directory of Community Blood Centers and Hospital Blood Banks [AABB. Directory of Community Blood Centers and Hospital Blood Banks. Bethesda, MD: AABB; 2009]. It finds that when materials define sex, the definition is "vaginal, oral, or anal sex whether or not a condom or other protection was used," and when materials define HIV/AIDS risk behaviors, the definition is, with few exceptions, "sexual contact with an infected person or by sharing needles or syringes used for injecting drugs." Widespread use of these definitions demonstrates the influence of "Making Your Blood Donation Safe." Through analysis of this document and variations upon it, this research finds that the category MSM therefore provides one component of the construction of HIV/AIDS as the providence of MSM, together with heterosexual Africans and other risk populations, conflating group membership with individual risk. Deferring MSM therefore fails as a behavior-based deferral because it collapses multiple sexual behaviors with varying risks into a single risk category. It constructs all MSM as HIV positive and implicitly constructs non-MSM as risk-free. PMID:22749059

  9. Effect of blood bank storage on the rheological properties of male and female donor red blood cells.

    Science.gov (United States)

    Daly, Amanda; Raval, Jay S; Waters, Jonathan H; Yazer, Mark H; Kameneva, Marina V

    2014-01-01

    It was previously demonstrated that red blood cell (RBC) deformability progressively decreases during storage along with other changes in RBC mechanical properties. Recently, we reported that the magnitude of changes in RBC mechanical fragility associated with blood bank storage in a variety of additive solutions was strongly dependent on the donor gender [15]. Yet, the potential dependence of changes in the deformability and relaxation time of stored blood bank RBCs on donor gender is not known. The objective of this study was to determine the effects of donor gender and blood bank storage on RBC deformability and relaxation time through the measurement of RBC suspension viscoelasticity. Packed RBC units preserved in AS-5 solution from 12 male and 12 female donors (three from each ABO group) were obtained from the local blood center and tested at 1, 4 and 7 weeks of storage at 1-6°C. At each time point, samples were aseptically removed from RBC units and hematocrit was adjusted to 40% before assessment of cell suspension viscoelasticity. RBC suspensions from both genders demonstrated progressive increases (p blood bank storage may reduce tissue perfusion and RBC lifespan in patients receiving blood bank RBCs.

  10. Analysis of Pre-Donation Deferral Causes in Blood Donors at a Rural Medical Institute of Rohilkhand Region, India

    OpenAIRE

    Jaiswal, Milan; Maheshwari, Sonam; Saxena, Ratna; Yadav, Pooja

    2016-01-01

    Aims: Deferral of potential blood donors due to various reasons at the time of pre-donation medical examination and interrogation leads to the scarcity of blood for the treatment of patients requiring blood transfusion. The aim of this study was to analyze the reasons for deferral and the demographic profile of these deferred blood donors, so that necessary changes in donor recruitment policies could be made in future.Methods: Deferral record of five years from January 2010 to December 2014 w...

  11. Epidemiology of hepatitis C virus. A preliminary study in volunteer blood donors.

    Science.gov (United States)

    Stevens, C E; Taylor, P E; Pindyck, J; Choo, Q L; Bradley, D W; Kuo, G; Houghton, M

    1990-01-01

    In a survey carried out from 1985 through 1986, volunteer blood donors to The Greater New York Blood Program were tested for two surrogate markers for non-A, non-B hepatitis--elevation of alanine aminotransferase level and presence of antibody to hepatitis B core antigen. Stored serum samples from selected donors were also recently tested for antibody to hepatitis C virus (anti-HCV). Anti-HCV was detected in 0.9% to 1.4% of donors and was higher in black and Hispanic donors than in white donors. Anti-HCV prevalence increased with increasing age through the fourth decade of life, but decreased thereafter, possibly reflecting the disappearance of detectable antibody with time. Anti-HCV correlated with both alanine aminotransferase level and the presence or absence of antibody to hepatitis B core antigen. These associations suggest that donor screening for elevation of alanine aminotransferase level and presence of antibody to hepatitis B core antigen was, as expected, at least partially effective in preventing transfusion-associated non-A, non-B hepatitis. The detection of anti-HCV in donors who have neither an elevation of alanine aminotransferase level nor presence of antibody to hepatitis B core antigen suggests that donor screening for anti-HCV will further reduce the risk of transfusion-associated hepatitis. PMID:2104548

  12. AN EVALUATION OF BLOOD DONOR DEFERRAL CAUSES AT TERTIARY CARE CENTRE OF SRIKAKULAM DISTRICT

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    Kalivarapu

    2016-03-01

    Full Text Available BACKGROUND Analysis of donor deferral pattern indicates the impact of knowledge of deferral criteria in blood donor. Donor deferral causes varies from region to region, hence the need to study the pattern of donor deferral. AIM OF THE STUDY To know the prevalence of donor deferral and the various causes responsible for them in our Institute. METHODS AND MATERIALS Retrospective study from January 2013 to August 2015. Demographic details and the various causes of permanent and temporary deferral were recorded and analysed. RESULTS Total number of deferral donors was 7% with more number of cases in females. The deferral donors with permanent causes were 19.7%, commonest being symptoms suggestive of HIV (26.14%. The deferral donors with temporary causes were 80.28%, commonest being underweight and anaemia. CONCLUSION Our Institute caters to the needs of rural and tribal population. To decrease the deferral rate and decrease the number of cases with symptoms suggestive of HIV, more emphasis has to make in creating awareness regarding the criteria for blood transfusion and self-deferral.

  13. Human immunodeficiency virus test-seeking blood donors in a large blood bank in Sao Paulo, Brazil.

    OpenAIRE

    Goncalez, T; Sabino, E; Sales, N; Chen, YH; D. Chamone; Busch, M.; Murphy, E; Custer, B; McFarland, W

    2010-01-01

    BACKGROUND: Persons with human immunodeficiency virus (HIV) risk behaviors are excluded from donation to reduce the risk of transfusion-transmitted infection. Persons donating to be tested for HIV may therefore deny risk behaviors. STUDY DESIGN AND METHODS: A random sample of donors completed a survey on motivations, knowledge, and attitudes on the screening process. Donors were considered test seekers if they agreed with two statements "I think that blood donation is a good, fast, and anonym...

  14. Motivations for Giving of Alumni Donors, Lapsed Donors and Non-Donors: Implications for Christian Higher Education

    Science.gov (United States)

    Rugano, Emilio Kariuki

    2011-01-01

    This descriptive and causal comparative study sought to identify motivations for alumni donor acquisition and retention in Christian institutions of higher learning. To meet this objective, motivations for alumni donors, lapsed donors, and non-donors were analyzed and compared. Data was collected through an electronic survey of a stratified sample…

  15. Predictors of iron levels in 14,737 Danish blood donors

    DEFF Research Database (Denmark)

    Rigas, Andreas Stribolt; Sørensen, Cecilie Juul; Pedersen, Ole Birger;

    2013-01-01

    Dietary studies show a relationship between the intake of iron enhancers and inhibitors and iron stores in the general population. However, the impact of dietary factors on the iron stores of blood donors, whose iron status is affected by blood donations, is incompletely understood....

  16. Hepatitis B, hepatitis C, HIV, and VDRL seroprevalence of blood donors in Mersin, Turkey

    OpenAIRE

    Öner, Seva; YAPICI, Gülçin; Şaşmaz, Caferi Tayyar; KURT, Ahmet Öner; BUĞDAYCI, Resul

    2011-01-01

    The aim of this study was to determine the seroprevalence and affecting factors of Hepatitis B surface antigen (HbsAg), hepatitis C virus antibody (anti-HCV), anti human immunodeficiency virus (anti-HIV), and VDRL in blood donors in Mersin. Materials and methods: This was a retrospective, descriptive study. All blood centers in Mersin were included in the study. The medical records of 30,716 blood donors were evaluated. The data were analyzed using descriptive analysis, linear-by-linear ass...

  17. Radio Immuno Assay (RIA) for detection of hepatitis B virus in blood donors

    International Nuclear Information System (INIS)

    Hepatitis B is a blood-borne viral disease which affects more than 250 million people world-wide, mostly in the third world. The disease can cause fatal liver cancer in adult life, if infected when young. This deals with the laboratory techniques used in the detection of hepatitis B virus of the donor blood. Natural blood transfusion service of Sri Lanka screens the hepatitis B surface antigen using the RIA technique to find out whether the normal healthy donors without a history of hepatitis are infected with hepatitis B virus

  18. Trends in sociodemographic and behavioral characteristics of HIV antibody-positive blood donors.

    Science.gov (United States)

    Cleary, P D; Van Devanter, N; Rogers, T F; Singer, E; Avorn, J; Pindyck, J

    1991-01-01

    This paper describes the sociodemographic characteristics of people who donated blood to the New York Blood Center between April 1985 and February 1988 and tested positive for antibodies to HIV. Information on HIV-related risk factors and knowledge of blood screening is presented for seropositive donors who participated in an evaluation study. The most commonly reported risk factor among men was sexual contact with another man, and many of the male seropositive donors reported sex with an intravenous (IV) drug user or use of IV drugs. The proportion of men reporting sexual contact with another man decreased over the period of the study, and the proportion reporting use of IV drugs or sex with an IV drug user increased. Awareness of blood screening for HIV antibodies increased over the study period. The greatest increase was among those donating for transfusion, but only about a quarter of seropositive donors used the confidential unit exclusion (CUE) process. PMID:2036292

  19. [Prevalence for seropositivity for HIV, hepatitis B and hepatitis C in blood donors].

    Science.gov (United States)

    Rivera-López, María Rebeca F; Zavala-Méndez, Celia; Arenas-Esqueda, Alfonso

    2004-01-01

    Despite utilizing different actions to render blood safe for transfusions, we continue to have the risk of transmitting some viral infections. For this reason, it is important to determine prevalence of infections due to HIV and hepatitis B and hepatitis C viruses in blood donors. Previous studies from Mexico indicate that HIV prevalence is 0.01 to 0.13%, while it is 0.11 to 1.22% for hepatitis B, and for hepatitis C, prevalence is 0.47 to 1.47%. We are checking the results of the screening tests (ELISA 3rd generation and chemiluminescent immunoassays) from blood donors studied at the Central Blood Bank (Banco Central de Sangre) at the Mexican Institute of Social Security's (IMSS) Twentieth First Century National Medical Center in Mexico City from 1995 to 2002. Reactive results were studied by confirmatory tests, Western Blot for HIV, AgHBs neutralization test for hepatitis B, and RIBA-HCV3.0 for hepatitis C. Reactive results from 513,062 blood donors confirmed for HV were 0.07%, reactive results and confirmation of hepatitis B from 511,733 blood donors were 0.13%, and reactive results and confirmation of hepatitis C from 511,115 blood donors were 0.31%. Rates obtained are low when compared with results of previous studies in Mexico for HIV, hepatitis B, and hepatitis C. It may be possible than these low rates indicate the positive impact obtained from preventive actions, better strategies of detection of blood donors with high risk, and the advantage of working with a fully automated test system with state-of-the-art technology. PMID:15633577

  20. Possibility of enhanced risk of retinal neovascularization in repeated blood donors: blood donation and retinal alteration

    Directory of Open Access Journals (Sweden)

    Rastmanesh R

    2011-09-01

    Full Text Available Reza RastmaneshDepartment of Clinical Nutrition and Dietetics, Shahid Beheshti University of Medical Sciences, National Nutrition and Food Technology Research Institute, Tehran, IranAbstract: Repeated blood donors manifest clinical, subclinical, and biochemical signs of iron deficiency anemia, have significantly higher erythropoietin and vascular endothelial growth factor (VEGF concentrations, and decreased tissue oxygen saturation, oxygenated tissue hemoglobin, and regional cerebral oxygen saturation. Erythropoietin and VEGF are potent retinal angiogenic factors which may initiate and promote the retinal angiogenesis process independently or simultaneously. Increases in circulating levels of erythropoietin and VEGF are proportionate to the levels of hematocrit, hypoxemia, and tissue hypoxia. It is suggested that higher erythropoietin production following iron deficiency anemia-induced chronic hypoxemia/hypoxia may, hypothetically, enhance the risk of retinal angiogenesis and/or neovascularization, possibly by inducing hypoxia inducible factor-1 alpha, which consequently upregulates genes stimulating angiogenesis, resulting in formation of a new vasculature, possibly by modulation of signal transducer and activator of transcription 3 signaling in the retina. Implications of this hypothesis cover erythropoietin doping, chronic hypoxia, and hypoxemic situations, such as angiogenesis-related cardiac and pulmonary diseases.Keywords: repeated blood donation, erythropoietin, retinal neovascularization, vascular endothelial growth factor, hypoxia

  1. Effect of Blood Donor Characteristics on Transfusion Outcomes: A Systematic Review and Meta-Analysis.

    Science.gov (United States)

    Chassé, Michaël; McIntyre, Lauralyn; English, Shane W; Tinmouth, Alan; Knoll, Greg; Wolfe, Dianna; Wilson, Kumanan; Shehata, Nadine; Forster, Alan; van Walraven, Carl; Fergusson, Dean A

    2016-04-01

    Optimal selection of blood donors is critical for ensuring the safety of blood products. The current selection process is concerned principally with the safety of the blood donor at the time of donation and of the recipient at the time of transfusion. Recent evidence suggests that the characteristics of the donor may affect short- and long-term transfusion outcomes for the transfused recipient. We conducted a systematic review with the primary objective of assessing the association between blood donor characteristics and red blood cell (RBC) transfusion outcomes. We searched MEDLINE, EMBASE, and Cochrane Central databases and performed manual searches of top transfusion journals for all available prospective and retrospective studies. We described study characteristics, methodological quality, and risk of bias and provided study-level effect estimates and, when appropriate, pooled estimates with 95% confidence intervals using the Mantel-Haenszel or inverse variance approach. The overall quality of the evidence was graded using Grading of Recommendations Assessment, Development and Evaluation (GRADE) methodology. From 6121 citations identified by our literature search, 59 studies met our eligibility criteria (50 observational, 9 interventional). We identified the evaluation of association of 17 donor characteristics on RBC transfusion outcome. The risk of bias and confounding of the included studies was high. The quality of evidence was graded as very low to low for all 17 donor characteristics. Potential associations were observed for donor sex with reduced survival at 90 days and 6 months in male recipients that receive donated blood from females (hazard ratio 2.60 [1.09, 6.20] and hazard ratio 2.40 [1.10, 5.24], respectively; n = 1), Human Leukocyte Antigen - antigen D Related (HLA-DR) selected transfusions (odds ratio [OR] 0.39 [0.15, 0.99] for the risk of transplant alloimmunization, n = 9), presence of antileukocyte antibodies (OR 5.84 [1.66, 20.59] for risk

  2. Blood donation and cardiovascular disease. Addressing the healthy donor effect

    NARCIS (Netherlands)

    Peffer, K.

    2015-01-01

    In this thesis, the possible protective effect of frequent blood donation on cardiovascular disease was studied. Previous research has shown that high iron stores could have damaging effects on developing cardiovascular disease, and that blood donation lowers iron stores. Lowering iron stores throug

  3. A SCREENING RESEARCH OF PLASMA BLOOD DONORS FOR MARKERS PARVOVIRUS INFECTION

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    Anastassia Ya. Antipova

    2015-01-01

    Full Text Available Parvovirus B19 (PV B19 replicates predominantly in progenitor cells of human erythrocytes and is transmitted by an airborne, vertical through and through blood or infected tissues. At-risk are pregnant women, people with immunodeficiency of different nature and individuals who need blood transfusions or organ transplantation. The available data indicate a high risk of infection through transfusion of blood containing the DNA of parvovirus B19, with viral load 105 copies/ml and above (Hourfar M.K. et al., 2011. According to the requirements of national regulations, the production of therapeutic drugs from plasma assumes the use of raw materials, free from viruses or with minimal viral load (Filatova E.C. et al., 2011. In some foreign countries a study of donor blood for the presence of DNA PV B19 is required; in our country the need for such screening is discussed (Giburt E.B. et al., 2013. Due to the fact that parvovirus is resistant to the methods of blood products desinfection, it is especially important to assess the quality of donor blood. Objective: To investigate the prevalence of the two markers parvovirus infection (IgG and PV B19 DNA in blood samples from one of the blood centers at St. Petersburg. Plasma samples from 100 blood donors from Military Medical Academy blood centre were tested by ELISA for the presence of IgG antibodies of parvovirus B19. Positive samples were tested by PCR for the DNA of parvovirus B19. ELISA test system recomWell Parvovirus B19 IgG (Microgen GmbH, Germany and diagnostic kits of Federal State Institution of Science «Central research Institute for epidemiology» of Rospotrebnadzor (Moscow, Russia which are approved for use in RF was used according to the manufacturers instructions. It was shown that 78 out of 100 donors aged 18 to 58 years had IgG-antibodies.76 positive blood plasma samples were investigated by PCR, with the 19 donors have found DNA of parvovirus B19 (25%. Viral load of one donor was 106

  4. [Antibody production in the blood of donors immunized with staphylococcal anatoxin].

    Science.gov (United States)

    Dyzyk, H M; Shumeha, I S; Tarasenko, A O; Patoka, V V

    1998-01-01

    Blood serum content was studied of specific antistaphylococcal antibodies (staphylolysins) in 576 donors immunized with staphylococcal anatoxin with the purpose of obtaining an antistaphylococcal plasma and antistaphylococcal immunoglobulin to be used in clinical settings. 292 donors had been immunized and examined prior to 1986, 284--after 1986 (before 1994). Comparison of the immune responses in the above periods of time permitted finding out that 13.03% of immunized donors responded to the antigenic stimulus by such paradoxical reaction as disappearance of specific antibodies; the number of persons-active respondents has gotten reduced from 17.12% to 5.98% as has the number of individuals having the baseline level of staphylolysins (1-2 ME/ml). The changes were at their greatest in donors with group A (II) blood. PMID:9621632

  5. Tattoos and transfusion-transmitted disease risk: implications for the screening of blood donors in Brazil

    Directory of Open Access Journals (Sweden)

    Sérgio de A. Nishioka

    2002-08-01

    Full Text Available Having a tattoo has been associated with serological evidence of hepatitis B and C viruses, as well as human immunodeficiency virus infections and syphilis; all of these are known to be transmissible by blood transfusion. These associations are of higher magnitude for individuals with nonprofessionally-applied tattoos and with two or more tattoos. Tattoos are common among drug addicts and prisoners, conditions that are also associated with transfusion-transmitted diseases. We examined the implications of these associations for the screening of blood donors in Brazil. Numbers of individuals who would be correctly or unnecessarily deferred from blood donation on the basis of the presence of tattoos, and on their number and type, were calculated for different prevalence situations based on published odds ratios. If having a tattoo was made a deferral criterion, cost savings (due to a reduced need for laboratory testing and subsequent follow-up would accrue at the expense of the deferral of appropriate donors. Restricting deferral to more `at-risk' sub-groups of tattooed individuals would correctly defer less individuals and would also reduce the numbers of potential donors unnecessarily deferred. Key factors in balancing cost savings and unnecessary deferrals include the magnitude of the pool of blood donors in the population, the prevalence of individuals with tattoos and the `culture' of tattoos in the population. Tattoos can therefore be an efficient criterion for the screening of blood donors in certain settings, a finding that requires corroboration from larger population-based studies.

  6. Blood donor deferral: time for change? An evidence-based analysis

    Directory of Open Access Journals (Sweden)

    Borra V

    2016-05-01

    Full Text Available Vere Borra,1 Giovani Vandewalle,1 Hans Van Remoortel,1 Veerle Compernolle,1,2 Emmy De Buck,1 Philippe Vandekerckhove1–31Belgian Red Cross-Flanders, Mechelen, 2Faculty of Medicine, University of Ghent, Ghent, 3Department of Public Health and Primary Care, Faculty of Medicine, KU Leuven, Leuven, BelgiumAbstract: Donor selection remains an important part in the safety of the blood supply all over the world. Yet, donor deferral criteria seem to be strongly based on the precautionary principle protecting safety and quality, and on supply and expense considerations. This review therefore provides an overview of the available evidence on donor exclusion criteria, as well as on their cost-effectiveness, for the most frequent reasons of donor deferral in our region. PubMed was queried to retrieve primary research studies, systematic reviews, and health technology assessments (HTAs concerning donor exclusion criteria. With a similar approach, HTAs about the different blood-banking safety interventions were included. Reasons for donor deferral were recorded via the blood bank information system of the Belgian Red Cross-Flanders. Seven systematic reviews were identified: four on donor safety (hypotension, hypertension/type 2 diabetes, epilepsy, and higher age and three on recipient safety (hemochromatosis, men who have sex with men, and endoscopy. Forty-three low-quality observational studies were included, as well as 16 HTAs: three about donor exclusion criteria and 13 cost-utility analyses about blood-banking safety interventions. In general, the available evidence for deferral reasons was of low quality, and for 60% of the top 30 reasons for excluding donors, no evidence was found. Blood banking shows its unique position as many safety measures far exceed the normally accepted cost of €50,000/quality-adjusted life-years. The historical model based on the precautionary principle and on supply and expense considerations provides adequate supplies of

  7. About hematopoietic properties of peripheral blood lymphocytes RNA from patients with polycythemia vera and healthy donors

    OpenAIRE

    A. G. Babaeva; N. M. Gevorkyan; Tishevskaya, N. V.; L. L. Golovkina; Yu. O. Muratova; A. A. Ragimov

    2015-01-01

    Total RNA isolated from peripheral blood lymphocytes of donor and patient with polycythemia, stimulates hematopoiesis in rats with toxic aplastic anemia due to benzene administration. Total RNA of lymphocytes from polycythemia patient has a more pronounced effect on the erythroid, myeloid and megakaryocytic hematopoiesis comparing to total RNA from donor lymphocytes. The greatest stimulatory effectof RNA observed after 21 days from the start of experiment. Total RNA of lymphocytes from polycy...

  8. TRENDS OF TRANSFUSION TRANSMITTABLE INFECTIONS AMONG VOLUNTARY BLOOD DONORS IN A TERTIARY CARE HOSPITAL, MANDYA

    OpenAIRE

    Manjunath; Mamatha P; Muralidhar Bhat; Shivakumar

    2014-01-01

    INTRODUCTION: Transmission of infectious diseases through donated blood is of concern to blood safety as transfusion forms an integral part of medical and surgical therapy. Blood transfusion carries the risk of transfusion-transmissible infections including HIV, hepatitis etc. Screening of voluntary donors who represent healthy population serves as a predictor for these dreadful diseases in healthy population. MATERIALS AND METHODS: This retrospective study was conducted a...

  9. Blood donor notification and counseling: Our experience from a tertiary care hospital in India

    Directory of Open Access Journals (Sweden)

    Urvershi Kotwal

    2015-01-01

    Full Text Available Aims: To evaluate the response rate of transfusion-transmissible infection (TTI-reactive donors after notification of their abnormal test results for the year 2012. Materials and Methods: This study is an observational descriptive study performed in our department over a period of 1 year. We evaluated the response rate of TTI-reactive donors after notification of their abnormal test results over 1 year as per the existing strategy (three telephonic and two postal communications. Results: During the study period, among the annual donation of 15,322 units, 464 blood donors were found to be seroreactive. Of these 464 seroreactive cases, 47 were HIV positive, 284 were reactive for Hepatitis B surface antigen (HBsAg, 49 were Hepatitis C (HCV positive and 84 were VDRL reactive. The TTI-reactive donors (464 for various markers were contacted: 229 (49.4% telephonically and the remaining 235 (50.6% not contacted on phone were informed by post. Of the 229 contacted donors, the response rate was 98.2% as only 225 donors reported (221 on the first, three on second and one on the third call for one to one counseling. The remaining four non-responders were - one HIV and three HBsAg reactive. The remaining 235 (50.6% reactive donors did not respond to any communication. Conclusion: Donor notification and post-donation counseling are an essential aspect of the blood bank that entails provision of information on serological status, assess the impact of test results on the donor and finally referral for medical care. As in our data only 49.4% of the blood donors could be contacted successfully, incomplete demographic details was the major limiting factor in communicating with rest. Of the 229 contacted donors, the response rate was 98.2%. A large majority (94.75% of the notified donors in our study contacted their health care provider when given clear instructions to do so. These results are encouraging because they indicate that a major element of the

  10. Effectiveness of blood donor questionnaire directed at risk factor for transfusion transmitted infections in Pakistani population

    Directory of Open Access Journals (Sweden)

    Nuzhat Salamat

    2012-01-01

    Full Text Available Background: Deferring blood donors who admit to high-risk behavior on questioning are likely to eliminate those in window period for transfusion transmitted infections (TTI. However, many questions have been implemented in some countries as part of donor history questionnaire, based on precautionary principle and not on evidence, and can result in increased donor losses. This study aims to identify effective risk-directed questions having high predictive value, in local context which can form part of blood donor deferral policies. For this, a case control study in a hospital blood bank having donation services was carried out prospectively over a period of three years. Materials and Methods: Two hundred and twenty donors, who were repeatedly reactive for HBsAg, anti-HCV, anti-HIV with EIA, and syphilis with TPHA, were the cases. Eight hundred and eighty four controls were the donors who tested negative for all TTI test. All donors answered seven hepatitis risk directed questions and their responses and reactivity status for TTI were used for statistical analysis with SPSS ver. 15. Results: Positive predictive value for history of jaundice at any age for HBsAg was 20%, while PPV for history of surgery in previous six months for both HBsAg and anti-HCVHCV was also around 20%, based on pretest probability of 7%. The post-test probability for these questions was around 30%. Odds ratios with 95% CI did not reveal any significant association of hepatitis with any of seven questions. Donor losses after deferring on basis of two questions were 5.3% per year, while deferral rate after all seven questions was 20%. Conclusions: Donors should be permanently deferred if there is history of jaundice at any age, while deferral period after surgery should be one year. Other risk-directed questions should not be used to defer donors. Donor deferral policies should be evidence based and questions with proven efficacy should be made part of donor history

  11. Ancestry informative markers and complete blood count parameters in Brazilian blood donors

    Directory of Open Access Journals (Sweden)

    Gabriela E. S. Felix

    2010-01-01

    Full Text Available A complete blood count is very useful in clinical diagnoses when reference ranges are well established for the population. Complete blood counts and allele frequencies of Ancestry Informative Markers (AIMs were analyzed in Brazilians with the aim of characterizing the hematological values of an admixed population. Positive associations were observed between gender and neutrophils, monocytes, eosinophils, erythrocytes, hemoglobin, hematocrit, MCV, MCHC and platelet counts. No significant differences were found for age, alcohol consumption, educational status, ethnicity, smoking in respect to the complete blood count values. In general, men had higher red blood cell values, while women had higher values for white blood cells and platelets. The study of the population was highly heterogeneous with mean proportions (± SE of African, European and Amerindian ancestry being 49.0 ± 3.0%, 44.0 ± 9.0% and 7.0 ± 9.0%, respectively. Amerindian ancestry showed limited contribution to the makeup of the population, but estimated ancestral proportions were statistically significant (r = 0.9838; P<0.001. These hematologic values are similar to Afro-Americans, another admixed population.

  12. Clinically Significant Minor Blood Group Antigens amongst North Indian Donor Population

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    Divjot Singh Lamba

    2013-01-01

    Full Text Available Background. Racial differences in blood group antigen distribution are common and may result in striking and interesting findings. These differences in blood group antigen distribution are important due to their influence on the clinical practice of transfusion medicine. Study Design and Methods. This is a prospective study, involving 1000 healthy regular repeat voluntary blood donors associated with the department. The clinically significant minor blood group antigens of these donors were studied. Results. Out of 1000 healthy regular repeat voluntary blood donors, 93% were D positive and 2.8% were K positive. Amongst the Rh antigens, e was the most common (99%, followed by D (93%, C (85.1%, c (62.3%, and E (21.5%. Within the MNS blood group system, antigen frequency was M (88%, N (57.5%, S (57.8%, and s (87.5%. Within the Duffy blood group system, antigen frequency was Fya (87.3% and Fyb (58.3%. Conclusions. This data base will help us to prevent alloimmunisation in young females, pregnant women, and patients who are expected to require repeated transfusions in life by providing them with antigen matched blood. Antigen negative blood can also be made available without delay to already alloimmunized multitransfused patients.

  13. Syphilis detection: evaluation of serological screening and pilot reverse confirmatory assay algorithm in blood donors.

    Science.gov (United States)

    Sommese, Linda; Paolillo, Rossella; Sabia, Chiara; Costa, Dario; De Pascale, Maria Rosaria; Iannone, Carmela; Esposito, Antonella; Schiano, Concetta; Napoli, Claudio

    2016-07-01

    Serological assays are still considered the most useful tests in the diagnosis of syphilis. Since no single serological assay is able to provide a satisfactory result, in our laboratory we have evaluated the usefulness of a commercially-available immunoblot to diagnose syphilis infection among blood donors. From October 2012 to June 2013, 4572 blood donors were screened for syphilis with an automated chemiluminescent microparticle immunoassay (CMIA). To confirm the presence of treponemal antibodies, CMIA-reactive sera were tested by standard Treponema pallidum haemagglutination assay (TPHA). In addition, an alternative confirmatory test - the immunoblot INNO-LIA assay was introduced in our laboratory. Since two additional positives among CMIA-reactive-TPHA-negative samples were found, we concluded that the INNO-LIA immunoblot allowed a better detection of syphilis compared to TPHA. A confirmatory strategy based on the use of two treponemal assays could meet the screening requirements for blood donors as well as in our centre. PMID:26068964

  14. Analysis of predonation loss of blood donors due to deferrals - in a tertiary care hospital set up

    OpenAIRE

    Shreedevi S Bobati; Vijaya Basavraj; Pallavi Prakash

    2016-01-01

    Context: Blood is a living tissue that is transfused into another human body and hence blood safety is a major issue in transfusion medicine. It is well-known that a large number of apparently healthy donors are deferred from successful blood donation because of varied reasons. They are the potential motivated donors the society is losing. Hence, it is very important to analyze the reasons for deferral and retain the motivated donors. Aims: The objective of this study was to determine the...

  15. Reduction of bioactive substances in stored donor blood: prestorage versus bedside leucofiltration

    DEFF Research Database (Denmark)

    Hammer, J H; Mynster, T; Reimert, C M;

    1999-01-01

    units, 9 were stored under standard conditions for 35 d, 9 were leucofiltered after donation and then stored for 35 d, and 3x9 units were stored for 7, 21 and 35 d, respectively, before leucofiltration. Samples were collected from blood units immediately after donation, and before and after......Leucocyte filtration has been suggested to improve transfusion products. We studied the effect of prestorage versus bedside leucofiltration on reduction of bioactive substances and leucocyte content in donor blood. Forty-five units of whole blood from healthy blood donors were studied. Of these...... leucofiltration, and analysed by ELISA and RIA methods for extracellular content of myeloperoxidase (MPO), eosinophil cationic protein (ECP), histamine (HIS) and plasminogen activator inhibitor-1 (PAI-1). Leucocyte content was counted in all samples. In non-filtered blood extracellular MPO, ECP, HIS and PAI-1...

  16. Trends in Transfusion Transmitted Infections Among Replacement Blood Donors in Karachi, Pakistan

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    Syed Mohammad Irfan

    2013-06-01

    Full Text Available OBJECTIVE: To determine the prevalence of Hepatitis-B, Hepatitis-C and Human Immunodeficiency infections in replacement blood donors. METHODS: From January 2004 to December 2011, 108,598 apparently healthy donors donated blood at our Blood Bank. Screening was done by Microparticle Enzyme Immuno Assay (MEIA method on Axsym System (Abbott Diagnostic, USA and in year 2011 by Chemiluminescent Immunoassay (CIA method on Architect i2000 (Abbott Diagnostic, USA. From 2010 onward, HIV reactive donors were advised for confirmatory tests and reported back with the results. RESULTS: Of the 108,598 total donors, 108,393 (99.8% were replacement donors with a mean age of 28.92 (17-55 years. Of this, only 164 (0.15% were females. Among the replacement donors, 4,906 (4.5% were found to be reactive for Hepatitis-B, C and Human Immunodeficiency Virus. All the reactive patients, except one, were males. HbsAg was positive in 2,068 (1.90% and anti-HCV in 2832 (2.61% donors, while 111 (0.10% were positive for Human Immunodeficiency Virus. Co-infectivity was observed in 103 (0.09% cases. The prevalence appeared to be higher in younger age group (17-30 yrs. Only 16.6% cases should be patients returned with results of the confirmatory tests for HIV and were found positive. CONCLUSION: Hepatitis-B and C sero-prevalence in our series of replacement donors appears high compared to most studies from neighboring countries and relatively low in comparison to earlier studies from Pakistan. Prevalence of HIV, however, appears low and turn out of HIV positive cases for confirmatory tests is low.

  17. Factors for viral infection in blood donors of South Kivu in the Democratic Republic of Congo

    OpenAIRE

    Kabinda, Jeff Maotela; Michèle, Dramaix-Wilmet; Donnen, Philippe; Miyanga, Serge Ahuka; van den Ende, Jef

    2014-01-01

    Introduction Assessing the knowledge, attitudes, practices and behaviors among blood donors in South Kivu and identify risk factors for viral markers. Methods A descriptive and analytical cross-sectional study involved 595 blood donors in the city of Bukavu (Head city of the province of South Kivu) in the eastern Democratic Republic of Congo. Results Our sample consisted of 70.3% men with a median age of 23 and 77% of young people fewer than 30 years. The score of knowledge and attitude of bl...

  18. Analysis of adverse events and predisposing factors in voluntary and replacement whole blood donors: A study from north India

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    Naveen Agnihotri

    2012-01-01

    Full Text Available Background: Lack of awareness and community motivation, compounded with fragmented blood transfusion services in our country, Often leads to shortage of blood. Donor recruitment and retention are essential for ensuring adequate blood supply. However, adverse events (AEs in donors have a negative impact on donor return. Aims and Objectives: The present study was aimed to assess the frequency of AEs in whole blood donors and analyze the predisposing factors for AEs. Material and Methods: The study was conducted on allogeneic whole blood donors over a period of 14 months, i.e., from January 2002 to February 2003. A total of 37,896 donors were monitored for any AEs: 22587 (59.6% were voluntary donors (VD and 15,309 (40.4% were replacement donors (RD. Results: Overall reaction rate was 2.5% with vasovagal reactions constituting 63.5% and hematomas 35.0% of all reactions. Vasovagal reactions showed a significant association with young age, lower weight, first time donation status, female gender, and nature of blood donation camps. Amongst male donors, RDs had more reactions (P=0.03 than VDs. Majority of donors (85% with vasovagal reactions admitted to some fear or anxiety prior to donation. Hematoma formation occurred significantly more when less trained staff performed phlebotomy. Conclusion: Donor safety is an essential prerequisite to increase voluntary blood donation. AE analysis helps in identifying the blood donors at risk of donor reactions and adopting appropriate donor motivational strategies, pre-donation counseling, and care during and after donation.

  19. Dengue viremia in blood donors in Northern India: Challenges of emerging dengue outbreaks to blood transfusion safety

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    Sadhana Mangwana

    2015-01-01

    Full Text Available Backdround: Emerging infectious diseases pose threats to the general human population; including recipients of blood transfusions. Dengue is spreading rapidly to new areas and with increasing frequency of major outbreaks. Screening blood for dengue antigens in dengue-endemic countries would be costly and should, therefore, be recommended only after careful assessment of risk for infection and cost. Aim: A prospective study was conducted to establish the magnitude of the threat that dengue poses to blood safety where it is sporadic with seasonal variations, to quantify risk and to assess that whether screening is feasible and cost-effective. Materials and Methods: Nonstructural protein 1 (NS1 antigen test was done on 1709 donations during dengue outbreak in the months August to November 2013 as an additional test using Bio-Rad Platelia Dengue NS1AG test kit which is one step sandwich format microplate enzyme immunoassay using murine monoclonal antibodies for capture and revelation. Chi-square test was used to find statistical significance. Results and Conclusions: Majority cases were whole blood, replacement, male donors with 76.10% donors in <35 years age group. About 17.85% were single donor platelet donations. NS1 antigen in all donors was negative. In the past, dengue affected mainly children who do not donate blood. With the changing trend, mean age of infection increased affecting the population that does donate blood, further reducing blood donation pool. Further studies need to be done in different geographic regions of the country during dengue transmission season to establish maximum incidence of viremic donations, rates of transfusion transmission and clinical consequences in recipients. If risk is found to be substantial, decision will be taken by the policymakers at what threshold screening should be instituted to ensure safe blood transfusion.

  20. Electrocardiographic abnormalities in Trypanosoma cruzi seropositive and seronegative former blood donors.

    Directory of Open Access Journals (Sweden)

    Antonio L Ribeiro

    Full Text Available BACKGROUND: Blood donor screening leads to large numbers of new diagnoses of Trypanosoma cruzi infection, with most donors in the asymptomatic chronic indeterminate form. Information on electrocardiogram (ECG findings in infected blood donors is lacking and may help in counseling and recognizing those with more severe disease. OBJECTIVES: To assess the frequency of ECG abnormalities in T.cruzi seropositive relative to seronegative blood donors, and to recognize ECG abnormalities associated with left ventricular dysfunction. METHODS: The study retrospectively enrolled 499 seropositive blood donors in São Paulo and Montes Claros, Brazil, and 483 seronegative control donors matched by site, gender, age, and year of blood donation. All subjects underwent a health clinical evaluation, ECG, and echocardiogram (Echo. ECG and Echo were reviewed blindly by centralized reading centers. Left ventricular (LV dysfunction was defined as LV ejection fraction (EF<0.50%. RESULTS: Right bundle branch block and left anterior fascicular block, isolated or in association, were more frequently found in seropositive cases (p<0.0001. Both QRS and QTc duration were associated with LVEF values (correlation coefficients -0.159,p<0.0003, and -0.142,p = 0.002 and showed a moderate accuracy in the detection of reduced LVEF (area under the ROC curve: 0.778 and 0.790, both p<0.0001. Several ECG abnormalities were more commonly found in seropositive donors with depressed LVEF, including rhythm disorders (frequent supraventricular ectopic beats, atrial fibrillation or flutter and pacemaker, intraventricular blocks (right bundle branch block and left anterior fascicular block and ischemic abnormalities (possible old myocardial infarction and major and minor ST abnormalities. ECG was sensitive (92% for recognition of seropositive donors with depressed LVEF and had a high negative predictive value (99% for ruling out LV dysfunction. CONCLUSIONS: ECG abnormalities are more

  1. Effectiveness of DNA-recombinant anti-hepatitis B vaccines in blood donors: a cohort study

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    Petry Andrea

    2007-11-01

    Full Text Available Abstract Background Although various studies have demonstrated efficacy of DNA-recombinant anti-hepatitis B vaccines, their effectiveness in health care settings has not been researched adequately. This gap is particularly visible for blood donors, a group of significant importance in the reduction of transfusion-transmitted hepatitis B. Methods This is a double cohort study of 1411 repeat blood donors during the period 1998–2002, involving a vaccinated and an unvaccinated cohort, with matching of the two in terms of sex, age and residence. Average follow-up was 3.17 person-years. The outcome measure was infection with hepatitis B virus (HBV, defined by testing positive on serologic markers HBsAg or anti-HBC. All blood donors were from the blood bank in Joaçaba, federal state of Santa Catarina, Brazil. Results The cohorts did not differ significantly regarding sex, age and marital status but the vaccinated cohort had higher mean number of blood donations and higher proportion of those residing in the county capital Joaçaba. Hepatitis B incidences per 1000 person-years were zero among vaccinated and 2,33 among non-vaccinated, resulting in 100% vaccine effectiveness with 95% confidence interval from 30,1% to 100%. The number of vaccinated persons necessary to avoid one HBV infection in blood donors was estimated at 429 with 95% confidence interval from 217 to 21422. Conclusion The results showed very high effectiveness of DNA-recombinant anti-HBV vaccines in blood donors. Its considerable variation in this study is likely due to the limited follow-up and the influence of confounding factors normally balanced out in efficacy clinical trials.

  2. Hepatitis E in blood donors: investigation of the natural course of asymptomatic infection, Germany, 2011

    Science.gov (United States)

    Vollmer, Tanja; Diekmann, Juergen; Eberhardt, Matthias; Knabbe, Cornelius; Dreier, Jens

    2016-01-01

    Asymptomatic hepatitis E virus (HEV) infections have been found in blood donors from various European countries, but the natural course is rarely specified. Here, we compared the progression of HEV viraemia, serostatus and liver-specific enzymes in 10 blood donors with clinically asymptomatic genotype 3 HEV infection, measuring HEV RNA concentrations, plasma concentrations of alanine/aspartate aminotransferase, glutamate dehydrogenase and bilirubin and anti-HEV IgA, IgM and IgG antibodies. RNA concentrations ranged from 77.2 to 2.19×105 IU/mL, with viraemia lasting from less than 10 to 52 days. Donors showed a typical progression of a recent HEV infection but differed in the first detection of anti-HEV IgA, IgM and IgG and seropositivity of the antibody classes. The diagnostic window between HEV RNA detection and first occurrence of anti-HEV antibodies ranged from eight to 48 days, depending on the serological assay used. The progression of laboratory parameters of asymptomatic HEV infection was largely comparable to the progression of symptomatic HEV infection, but only four of 10 donors showed elevated liver-specific parameters. Our results help elucidate the risk of transfusion-associated HEV infection and provide a basis for development of screening strategies. The diagnostic window illustrates that infectious blood donors can be efficiently identified only by RNA screening. PMID:27608433

  3. Prospective Audit of Blood Donor Selection Process in a Tertiary Care Hospital of a Developing Country

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    Naila Raza

    2016-05-01

    Full Text Available Objective: The aim of this study was to emphasize the significance of internal audits of the blood donor selection process and documentation in a resource-limited country by assessing compliance with the established protocols, and to identify weak areas in the process. Materials and Methods: This audit reviewed the donor selection process at the blood bank of Liaquat National Hospital & Medical College, Karachi, over a 6-month period. Seven variables selected as performance indicators were graded as very good (%90-100%, good (80%-89%, satisfactory (70%-79%, or unacceptable (<70%. Blood bank staff was asked for feedback and suggestions. Results: Documentation of donor demographics was not within the acceptable range (documentation rates of 65.14%, donor status records were satisfactory (77.64%, and donor physical exam records were graded as good (86.34%. Five performance indicators were graded as very good (90%-100%. Conclusion: The audit proved productive in identifying major causes of irregularities in documentation and in making valuable suggestions for their rectification.

  4. Prevalence of hepatitis A viral RNA and antibodies among Chinese blood donors.

    Science.gov (United States)

    Sun, P; Su, N; Lin, F Z; Ma, L; Wang, H J; Rong, X; Dai, Y D; Li, J; Jian, Z W; Tang, L H; Xiao, W; Li, C Q

    2015-12-09

    Like other developing countries, China was reported to have a relatively high seroprevalence of anti-hepatitis A antibodies (anti-HAV). However, no studies have evaluated the prevalence of anti-HAV and HAV RNA among voluntary blood donors with or without elevated serum alanine transaminase (ALT) levels. Anti-HAV antibodies were detected using an enzyme-linked immunosorbent assay, and reverse transcription quantitative polymerase chain reaction was carried out for detection of HAV RNA. In the current study, we analyzed a total of 450 serum samples with elevated ALT levels (≥40 U/L) and 278 serum samples with non-elevated ALT levels. Seroprevalence rates of anti-HAV were 51.6% in donors with elevated ALT and 41.4% in donors with non-elevated ALT; however, none of the samples was positive for HAV RNA. The results of our study showed lower seroprevalence rates of anti-HAV in blood donors (irrespective of ALT levels) than those in published data on Chinese populations. Although donors with elevated ALT had statistically higher prevalence rates of anti- HAV than did those with non-elevated ALT, none of the serum samples had detectable levels of the active virus. In conclusion, our results demonstrate that the transmission of hepatitis A by blood transfusion will occur rarely.

  5. Donation frequency, iron loss, and risk of cancer among blood donors

    DEFF Research Database (Denmark)

    Edgren, Gustaf; Reilly, Marie; Hjalgrim, Henrik;

    2008-01-01

    for cancers of the liver, lung, colon, stomach, and esophagus, which are thought to be promoted by iron overload (combined odds ratio [OR] = 0.70, 95% confidence interval [CI] = 0.58 to 0.84), but only among men and only with a latency of 3-7 years. The risk of non-Hodgkin lymphoma was higher among frequent......BACKGROUND: Long-term deleterious effects of repeated blood donations may be masked by the donors' healthy lifestyle. To investigate possible effects of blood donation and iron loss through blood donation on cancer incidence while minimizing "healthy donor effects," we made dose...... = 107140) were individually matched on sex, age, and county of residence. Using conditional logistic regression, we estimated relative risks of cancer according to number of blood donations made or estimated iron loss 3-12 years before a case patient was diagnosed with cancer. All statistical tests were...

  6. Character comparison of Weihai apheresis donors with whole blood donors%威海市机采献血者与全血献血者人群特征比较

    Institute of Scientific and Technical Information of China (English)

    王明静; 常爱荣; 谭玲丽

    2014-01-01

    Objective Population characteristics of apheresis donors is compared with that of whole blood donors in Weihai to provide reference for blood donor recruitment. Methods Voluntary blood donors information is organized by using information management system in blood station, the population structure, experiences and blood test results of 1674 apheresis platelet donors and 32089 whole blood donors in 2013 are analyzed by using statistical software spss18.0. Results Comparing apheresis donors with whole blood donors ,the differences of gender, age, educational level, occupation, experience of blood donation and blood test results have statistical significance. Conclusion The persent of apheresis donors under 35 years old is larger than that of whole blood donors, because of male physical characteristics and targeted recruitment. Differences in educational level and in occupation are caused by group blood donations. According to local conditions, blood station can develop promotional strategies and targeted recruitment in order to improve the success rate of recruitment ,so that donors ' team can go into a virtuous circle.%目的:比较威海市机采献血者与全血献血者人群特征,为献血者招募提供参考。方法利用血站信息管理系统对无偿献血者信息进行统计整理,利用SPSS18.0统计软件对2013年威海市1674名机采血小板捐献者、32089名全血捐献者人群结构、献血经历和血液检测结果进行分析。结果威海市机采献血者与全血献血者统计指标中,性别、年龄、文化程度、职业、献血经历、血液检测结果差异均具有统计学意义。结论机采献血者中35岁以下年轻男性比例明显高于全血献血者,分析原因与男性身体特征及工作人员针对性招募有关,机采献血者与全血献血者文化程度与职业的差异在于团体献血工作的开展。血站可根据本地特点,因地制宜地制订宣传策略,开展有针对

  7. Red Kidney: Kidney Transplant From a Deceased Donor Who Received Massive Blood Transfusion During Cardiopulmonary Bypass.

    Science.gov (United States)

    Bell, Richard; Hanif, Faisal; Prasad, Padmini; Ahmad, Niaz

    2016-06-01

    Here, we present a case of a deceased-donor kidney transplant. The brain-dead donor had received a massive blood transfusion during cardiopulmonary bypass, which lead to hemolysis, hemoglobinuria, acute kidney injury, and renal replacement therapy. The kidney appeared red after in situ flush. Postoperatively, the recipient developed delayed graft function. Protocol biopsy during the postoperative period revealed the widespread deposition of heme pigment in the renal tubules. Massive blood transfusion and cardiopulmonary bypass surgery are associated with hemolysis and heme pigment deposition in the renal tubules, which subsequently lead to acute kidney injury. Kidneys from such donors appear red and, while this does not preclude transplant, are likely to develop delayed graft function. PMID:26030717

  8. Prevalence of hepatitis B seromarkers and hepatitis C antibodies in blood donors in Basra, Iraq

    OpenAIRE

    Al-Rubaye, Ali; Tariq, Ziad; Alrubaiy, Laith

    2016-01-01

    Background Transfusion-caused hepatitis remains a major problem in Iraq. Therefore, testing for hepatitis B surface antigen (HBsAg), antibodies to hepatitis B core antigen (anti-HBc) and antibodies to hepatitis C antigen (anti-HCV) is a very important preventative measure. The objective of this study was to establish the prevalence of hepatitis B and C virus seromarkers among blood donors as a foundation for safe blood transfusion in Iraq. Methods A cross-sectional observational study was con...

  9. Prevention of post-transfusion hepatitis c transmission through donor blood and its components

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    A. V. Chechetkin

    2015-01-01

    Full Text Available The aim of organizational aspects of preventing the transmission of hepatitis C virus with donor blood and its components.Materials and methods. An activity of the blood service establishments in Russia for the prevention of HCV infection through transfusion of blood and its components on the basis of the analysis of sectoral statistical surveys was studied.Results. The frequency of detection of antibodies to hepatitis C virus in blood donors and its components during 2009–2013 decreased by more than 1,5 times. The percentage of donors who have identified markers of hepatitis C virus was significantly different in different regions: from 0,51% to 1,36%. The activity of the blood service implemented method of plasma quarantine resulting annually rejected from 0,32% to 0,23% as a result of the identified markers of HCV. Pathogen inactivated plasma volume increased in 3 times, the platelet concentrate in 3,2 times.Conclusion. To ensure the safety of donated blood and its components in the blood service effectively the modern technology use for to prevention transmission of the HCV: quarantine of plasma, donor selection and development, inactivation of pathogens. The degree of implementation in practice of nonpaid voluntary blood transfusions significantly increased and is characterized by regional features in recent years .

  10. Glucose-6-Phosphate Dehydrogenase Deficiency among Male Blood Donors in Sana’a City, Yemen

    Science.gov (United States)

    Al-Nood, Hafiz A.; Bazara, Fakiha A.; Al-Absi, Rashad; Habori, Molham AL

    2012-01-01

    Objectives To determine the prevalence of Glucose-6-phosphate dehydrogenase (G-6-PD) deficiency among Yemeni people from different regions of the country living in the capital city, Sana’a, giving an indication of its overall prevalence in Yemen. Methods A cross-sectional study was conducted among Yemeni male blood donors attending the Department of Blood Bank at the National Centre of the Public Health Laboratories in the capital city, Sana’a, Yemen. Fluorescent spot method was used for screening, spectrophotometeric estimation of G-6-PD activity and separation by electrophoresis was done to determine the G-6-PD phenotype. Results Of the total 508 male blood donors recruited into the study, 36 were G-6-PD deficient, giving a likely G-6-PD deficiency prevalence of 7.1%. None of these deficient donors had history of anemia or jaundice. Thirty-five of these deficient cases (97.2%) showed severe G-6-PD deficiency class II (<10% of normal activity), and their phenotyping presumptively revealed a G-6-PD-Mediterranean variant. Conclusion The results showed a significant presence of G-6-PD deficiency with predominance of a severe G-6-PD deficiency type in these blood donors in Sana’a City, which could represent an important health problem through occurrence of hemolytic anemia under oxidative stress. A larger sample size is needed to determine the overall prevalence of G-6-PD deficiency, and should be extended to include DNA analysis to identify its variants in Yemen. PMID:22359725

  11. Epidemiology of high-level parvovirus B19 viraemia among Dutch blood donors, 2003-2009

    NARCIS (Netherlands)

    K. Kooistra; H.J. Mesman; M. de Waal; M.H.G.M. Koppelman; H.L. Zaaijer

    2011-01-01

    Background and Objectives Plasma derivatives and blood components with low levels of parvovirus B19 (B19) seem not infectious, but recently infected, highly viraemic donors may transmit B19. We studied the incidence of high-level B19 viraemia (B19 DNA > 106 IU/ml) in 6 center dot 5 million Dutch blo

  12. A genetic risk factor for low serum ferritin levels in Danish blood donors

    DEFF Research Database (Denmark)

    Sørensen, Erik; Grau, Katrine; Berg, Trine;

    2012-01-01

    /or restless leg syndrome (RLS) were investigated in two groups of female blood donors. The first group had low iron stores (serum ferritin ≤ 12 µg/L, n = 657), and the second group had normal to high iron stores (serum ferritin > 30 µg/L, n = 645). Genotype distribution for each of the SNPs was compared...

  13. Dengue Virus Transmission by Blood Stem Cell Donor after Travel to Sri Lanka; Germany, 2013

    Centers for Disease Control (CDC) Podcasts

    2014-09-22

    Dr. Mike Miller reads an abridged version of the article, Dengue Virus Transmission by Blood Stem Cell Donor after Travel to Sri Lanka; Germany, 2013.  Created: 9/22/2014 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 10/8/2014.

  14. Protocol for a national blood transfusion data warehouse from donor to recipient

    NARCIS (Netherlands)

    van Hoeven, Loan R; Hooftman, Babette H; Janssen, Mart P; de Bruijne, Martine C; de Vooght, Karen M K; Kemper, Peter; Koopman, Maria M W

    2016-01-01

    INTRODUCTION: Blood transfusion has health-related, economical and safety implications. In order to optimise the transfusion chain, comprehensive research data are needed. The Dutch Transfusion Data warehouse (DTD) project aims to establish a data warehouse where data from donors and transfusion rec

  15. Malaria seroprevalence in blood bank donors from endemic and non-endemic areas of Venezuela

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    Carmen Elena Contreras

    2011-03-01

    Full Text Available In Venezuela, a total of 363,466 malaria cases were reported between 1999-2009. Several states are experiencing malaria epidemics, increasing the risk of vector and possibly transfusion transmission. We investigated the risk of transfusion transmission in blood banks from endemic and non-endemic areas of Venezuela by examining blood donations for evidence of malaria infection. For this, commercial kits were used to detect both malaria-specific antibodies (all species and malaria antigen (Plasmodium falciparum only in samples from Venezuelan blood donors (n = 762. All samples were further studied by microscopy and polymerase chain reaction (PCR. The antibody results showed that P. falciparum-infected patients had a lower sample/cut-off ratio than Plasmodium vivax-infected patients. Conversely, a higher ratio for antigen was observed among all P. falciparum-infected individuals. Sensitivity and specificity were higher for malarial antigens (100 and 99.8% than for antibodies (82.2 and 97.4%. Antibody-positive donors were observed in Caracas, Ciudad Bolívar, Puerto Ayacucho and Cumaná, with prevalences of 1.02, 1.60, 3.23 and 3.63%, respectively. No PCR-positive samples were observed among the donors. However, our results show significant levels of seropositivity in blood donors, suggesting that more effective measures are required to ensure that transfusion transmission does not occur.

  16. Does the Presence of a Measurable Blood Alcohol Level in a Potential Organ Donor Affect the Outcome of Liver Transplantation?

    OpenAIRE

    Hassanein, Tarek I.; GAVALER, JUDITH S.; Fishkin, David; Gordon, Robert; Starzl, Thomas E.; Van Thiel, David H.

    1991-01-01

    The widespread application of hepatic transplantation has created a tremendous demand for donor organs. An assessment of donor parameters is thought to be important in selecting good donors; however, the criteria utilized have not been standardized. This study was performed to determine the effect of a measurable donor blood alcohol level on graft survival. Fifty-two patients who underwent orthotopic liver transplantation at the University of Pittsburgh were included in the study. Twenty-five...

  17. Seroprevalence and Incidence of hepatitis E in blood donors in Upper Austria.

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    Carina Fischer

    Full Text Available In recent years various studies showed, that hepatitis E virus (HEV is a growing public health problem in many developed countries. Therefore, HEV infections might bear a transmission risk by blood transfusions. The clinical relevance still requires further investigations. The aim of this study was to provide an overview of acute HEV infections in Upper Austrian blood donors as well as a risk estimation of this transfusion-related infection.A total of 58,915 blood donors were tested for HEV RNA using a commercial HEV RT-PCR Kit. 7 of these donors (0.01% were PCR-positive with normal laboratory parameters in absence of clinical signs of hepatitis. Viral load determined by quantitative real-time PCR showed a HEV nucleic acid concentration of 2,217 293,635 IU/ml. At follow-up testing (2-11 weeks after donation all blood donors had negative HEV RNA results. Additionally, genotyping was performed by amplification and sequencing of the ORF1 or ORF2 region of the HEV genome. All HEV RNA positive donor samples revealed a genotype 3 isolate. For the antibody screening, anti-HEV IgM and IgG were detected by ELISA. Follow up serological testing revealed that no donor was seropositive for HEV IgM or IgG antibodies at time of donation. Moreover, we verified the prevalence of anti-HEV IgG in 1,203 of the HEV RNA negative tested blood donors. Overall 13.55% showed positive results for anti-HEV IgG.In the presented study, we investigated HEV infections in blood donations of Upper Austria over 1 year. We concluded that 1 out of 8,416 blood donations is HEV RNA positive. Seroprevalence of anti HEV IgG results in an age-related increase of 13.55%. Therefore, based on this data, we recommend HEV-PCR screening to prevent transmission of hepatitis E virus by transfusion.

  18. Prevalence and trend of major transfusion-transmissible infections among blood donors in Western China, 2005 through 2010.

    Directory of Open Access Journals (Sweden)

    Yan Song

    Full Text Available BACKGROUND: The prevalence of transfusion-transmissible infections (TTIs in blood donations is important for evaluating blood safety and potential risks to the population. This study investigated the prevalence of TTIs among blood donors in Western China and suggested measures for policy-makers. METHODS: The screening results of 66,311 donations between 2005 and 2010 from a central blood center in Western China were analyzed. The prevalence of hepatitis B virus (HBV, hepatitis C virus (HCV, human immunodeficiency virus (HIV, and syphilis infections were expressed in percentages for the entire study group as well as groups by demographic characteristics and donation frequency, with differences analyzed using Fisher's exact or Chi-square test. Logistic regression was performed to identify the influencing factors of the detected results. RESULTS: 1,769 (2.67%, 95% CI 2.55-2.79% of the donated blood had serological evidence of infection with at least one pathogen and 44 (0.07%, 95% CI 0.05-0.09% showed evidence of multiple infections. The seroprevalence of HBV, HCV, HIV, and syphilis infections was 0.87% (95% CI 0.80-0.94%, 0.86% (95% CI 0.79-0.93%, 0.31% (95% CI 0.26-0.35%, and 0.70% (95% CI 0.64-0.76% respectively. Trend analysis for the prevalence of TTIs showed a significant increase from 2.44% to 3.71% (χ2 = 100.72, p = 0.00 over this 6-year period. The positive rates for TTIs varied along demographic lines. The top three risk factors in test-positive donors were identified as age, education level and donation frequency. The older age group and lower educated group were linked to a higher prevalence of TTIs. A decreasing prevalence was associated with an increasing frequency of blood donations (χ2 = 562.78, p = 0.00. CONCLUSIONS: Hepatitis B and C were found most, and often in conjunction with syphilis. These were the primary threats to blood safety. The high positivity rate and the increasing prevalence of TTIs among blood

  19. Prevalence and Trend of Major Transfusion-Transmissible Infections among Blood Donors in Western China, 2005 through 2010

    Science.gov (United States)

    Song, Yan; Bian, Ying; Petzold, Max; Ung, Carolina Oi Lam

    2014-01-01

    Background The prevalence of transfusion-transmissible infections (TTIs) in blood donations is important for evaluating blood safety and potential risks to the population. This study investigated the prevalence of TTIs among blood donors in Western China and suggested measures for policy-makers. Methods The screening results of 66,311 donations between 2005 and 2010 from a central blood center in Western China were analyzed. The prevalence of hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV), and syphilis infections were expressed in percentages for the entire study group as well as groups by demographic characteristics and donation frequency, with differences analyzed using Fisher's exact or Chi-square test. Logistic regression was performed to identify the influencing factors of the detected results. Results 1,769 (2.67%, 95% CI 2.55–2.79%) of the donated blood had serological evidence of infection with at least one pathogen and 44 (0.07%, 95% CI 0.05–0.09%) showed evidence of multiple infections. The seroprevalence of HBV, HCV, HIV, and syphilis infections was 0.87% (95% CI 0.80–0.94%), 0.86% (95% CI 0.79–0.93%), 0.31% (95% CI 0.26–0.35%), and 0.70% (95% CI 0.64–0.76%) respectively. Trend analysis for the prevalence of TTIs showed a significant increase from 2.44% to 3.71% (χ2 = 100.72, p = 0.00) over this 6-year period. The positive rates for TTIs varied along demographic lines. The top three risk factors in test-positive donors were identified as age, education level and donation frequency. The older age group and lower educated group were linked to a higher prevalence of TTIs. A decreasing prevalence was associated with an increasing frequency of blood donations (χ2 = 562.78, p = 0.00). Conclusions Hepatitis B and C were found most, and often in conjunction with syphilis. These were the primary threats to blood safety. The high positivity rate and the increasing prevalence of TTIs among blood

  20. A Case of Living Donor Liver Transplant Recipient Treated With Novel Blood Purification “Plasma Diafiltration”

    OpenAIRE

    HAYASHI, HIRONORI; Takamura, Hiroyuki; Taniguchi, Takumi; Nakanuma, Shin-ichi; Nakagawara, Hisatoshi; Tajima, Hidehiro; KITAGAWA, HIROHISA; ONISHI, ICHIRO; Tani, Takashi; OHTA, TETSUO

    2013-01-01

    Blood purification therapy is indispensable for liver transplant recipients. The case of a living donor liver transplant recipient who represented graft insufficiency and was supported by novel blood purification “plasma diafiltration” immediately after operation is presented. A 60-year-old woman was referred for living donor liver transplant because of liver cirrhosis due to hepatitis C. Elective living donor liver transplant was performed, but the graft was small for size. Thus, the signs o...

  1. Comparison of deferral rates using a computerized versus written blood donor questionnaire: a randomized, cross-over study [ISRCTN84429599

    OpenAIRE

    Bourque Ronald; Haynes R Brian; Ali Anita; Swanson Graham; Hayward Robert; Sellors John W; Moore Karen-Ann; Lohfeld Lynne; Dalby Dawn; Howard Michelle

    2002-01-01

    Abstract Background Self-administered computer-assisted blood donor screening strategies may elicit more accurate responses and improve the screening process. Methods Randomized crossover trial comparing responses to questions on a computerized hand-held tool (HealthQuiz, or HQ), to responses on the standard written instrument (Donor Health Assessment Questionnaire, or DHAQ). Randomly selected donors at 133 blood donation clinics in the area of Hamilton, Canada participated from 1995 to 1996....

  2. External validation and updating of a Dutch prediction model for low hemoglobin deferral in Irish whole blood donors

    NARCIS (Netherlands)

    Baart, A.M.; Atsma, F.; McSweeney, E.N.; Moons, K.G.; Vergouwe, Y.; Kort, W.L. de

    2014-01-01

    BACKGROUND: Recently, sex-specific prediction models for low hemoglobin (Hb) deferral have been developed in Dutch whole blood donors. In the present study, we validated and updated the models in a cohort of Irish whole blood donors. STUDY DESIGN AND METHODS: Prospectively collected data from 45,031

  3. Low Prevalence of Brucella Agglutinins in Blood Donors in Central Province of Iran

    Directory of Open Access Journals (Sweden)

    Masoomeh Sofian

    2013-03-01

    Full Text Available Background: Brucellosis is a zoonotic disease of worldwide distribution and has great economic importance. Despite its control in many countries, it remains endemic in Iran. Brucellosis was investigated in many high risk occupational groups; however, few studies on the prevalence of brucellosis among blood donors are available. To determine the seroprevalence of brucellosis antibodies in blood donors, a serological study was carried out in central province of Iran.Materials and Methods: A total of 897 healthy blood donors with mean age 37.23 ± 10.9 years were enrolled in the study. Laboratory tests including Standard Tube Agglutination Test (STA and 2-mercaptoethanol (2ME agglutination were checked in all samples. STA dilution ≥ 1:80, and in the presence of 2-mercaptoethanol (2ME agglutination ≥ 20 was considered positive.Results: Out of 897 cases, 11.9% were inhabitants of rural areas. 41.5% had history of consumption of unpasteurized dairy products and 9.3% had history of contact with domestic animals. A very low level of Brucella agglutinins was present in 3(0.33% of the samples and only one sample (0.11% was found to be truly positive for Brucella agglutinins. 2ME was negative in all samples. None of these 4 subjects showed signs and symptoms of brucellosis in 6 months follow-up.Conclusion: On the basis of our data, brucellosis has no epidemiological and clinical importance in our blood donors; therefore, it is not recommended to perform screening tests such as, STA and 2ME to identify brucellosis antibodies in the sera of blood donors.

  4. Contribution of the Retrovirus Epidemiology Donor Study (REDS to research on blood transfusion safety in Brazil

    Directory of Open Access Journals (Sweden)

    Paula Loureiro

    2014-04-01

    Full Text Available The Retrovirus Epidemiology Donor Study (REDS program was established in the United States in 1989 with the purpose of increasing blood transfusion safety in the context of the HIV/AIDS and human T-lymphotropic virus epidemics. REDS and its successor, REDS-II were at first conducted in the US, then expanded in 2006 to include international partnerships with Brazil and China. In 2011, a third wave of REDS renamed the Recipient Epidemiology and Donor Evaluation Study-III (REDS-III was launched. This seven-year research program focuses on both blood banking and transfusion medicine research in the United States of America, Brazil, China, and South Africa. The main goal of the international programs is to reduce and prevent the transmission of HIV/AIDS and other known and emerging infectious agents through transfusion, and to address research questions aimed at understanding global issues related to the availability of safe blood. This article describes the contribution of REDS-II to transfusion safety in Brazil. Articles published from 2010 to 2013 are summarized, including database analyses to characterize blood donors, deferral rates, and prevalence, incidence and residual risk of the main blood-borne infections. Specific studies were developed to understand donor motivation, the impact of the deferral questions, risk factors and molecular surveillance among HIV-positive donors, and the natural history of Chagas disease. The purpose of this review is to disseminate the acquired knowledge and briefly summarize the findings of the REDS-II studies conducted in Brazil as well as to introduce the scope of the REDS-III program that is now in progress and will continue through 2018.

  5. Action plan to regain unnecessary deferred blood donors due to malaria risk in Turkey.

    Science.gov (United States)

    Değirmenci, Aysu; Döşkaya, Mert; Caner, Ayşe; Nergis, Sebnem; Gül, Kadri; Aydınok, Yeşim; Ertop, Tufan; Aksoy, Nurten; Korkmaz, Metin; Alkan, Mehmet Ziya; Üner, Ahmet; Gürüz, Yüksel

    2012-06-01

    Malaria was expected to be a major problem during blood donation in Turkey due to existence of malaria cases in southeastern region of Turkey. The present study aimed for the first time, to investigate malaria in "donors deferred for malaria risk" and to determine the regional rates of malaria deferral in Turkey. Blood samples were collected from several Blood Banks of southeastern provinces where local malaria cases still exist and from Blood Bank of Ege University Medical School (EUMS) located in western Turkey where malaria is eradicated decades ago. Plasmodium spp. and specific antibodies were investigated by stained smears, antigen detection, PCR and ELISA. Among the donors deferred for malaria risk, Plasmodium spp. were not detected by microscopy, PCR or antigen detection. Seroprevalances were 2% and 3.92% in western and southeastern regions, respectively. Rate of donor deferral for malaria risk was 0.9% in EUMS and deferrals were exclusively because of travel to southeastern Turkey. In southeastern provinces, deferrals were mainly due to malaria like fever history. The present study first time assessed regional rates of donor deferral due to malaria risk in Turkey. Previously, malaria was expected to be a major problem during blood donation in Turkey due to existence of malaria cases in southeastern region of Turkey. The results of the study showed that 97% of the deferrals were unnecessary. In conclusion, to reduce unnecessary donor deferrals in Turkey, in addition to comprehensive questioning for malaria history, the usage of a malaria antibody screening method should be initiated prior to deferral decision.

  6. Trend of Hepatitis B Surface Antigen (HBsAg) among blood donors at the blood bank of a tertiary care referral teaching hospital in Southern India

    OpenAIRE

    Yashovardhan A.; Sreedhar Babu K.V.; Jothi Bai D.S.

    2015-01-01

    Background: Blood is a scarce, but lifesaving resource; it is also the most efficient vehicle for the transmission of Hepatitis B virus (HBV). Hence there is a need for accurate screening of hepatitis B surface antigen (HBsAg) among blood donors. The present study was designed to assess the seroprevalence of HBsAg, among the voluntary and replacement blood donors in the blood bank of a tertiary care referral teaching hospital in Andhra Pradesh. Methods: This is a prospective cross sectiona...

  7. Reference range for T lymphocytes populations in blood donors from two different regions in Brazil

    Directory of Open Access Journals (Sweden)

    A.J.L. Torres

    2009-06-01

    Full Text Available This study defined the normal variation range for different subsets of T-lymphocyte cells count in two different Brazilian regions. We analysed the T-lymphocytes subpopulations (CD3+, CD4+, CD8+ in blood donors of two Brazilian cities, located in North (Belem, capital state of Para, indian background and Northeast (Salvador, capital state od Bahia, African background regions of Brazil. Results were compared according to gender, stress level (sleep time lower than 8 hours/day, smoking, and alcohol intake. Lymphocytes subpopulations were measured by flow cytometry. Five hundred twenty-six blood donors from two Brazilians cities participated in the study: 450 samples from Bahia and 76 samples from Pará. Most (60% were men, 59% reported alcohol intake, 12% were smokers, and 80% slept at least 8 h/day. Donors from Bahia presented with significantly higher counts for all parameters, compared with Para. Women had higher lymphocytes levels, in both states, but only CD4+ cells count was significantly higher than men's values. Smokers had higher CD4+ counts, but sleep time had effect on lymphocytes levels only for Para's donors (higher CD3+ and CD4+ counts. That state had also, a higher proportion of donors reporting sleep time <8 h/day. The values for CD3, CD4 and CD8+ cells count were significantly higher in blood donors from Bahia than among those from Pará. Female gender, alcohol intake, stress level, and smoking were associated with higher lymphocyte counts. The use of a single reference range for normal lymphocytes count is not appropriate for a country with such diversity, like Brazil is.

  8. Prevalence and Incidence of Syphilis among Volunteer Blood Donors in Israel

    Directory of Open Access Journals (Sweden)

    Leibovici Vera

    2014-01-01

    Full Text Available Data of 1,290,222 volunteer blood donors, in a 5-year period, was analyzed for prevalence and incidence of syphilis. Subsequent testing of donations positive in Treponema pallidum hemagglutination assay included Venereal Disease Research Laboratory and fluorescent Treponemal antibody absorption. Stepwise logistic regression model was used to identify positive syphilis serology. Prevalence of syphilis was 47 : 100,000, similar in men and women and increased significantly with age (P<0.001. Native Israelis had the lowest prevalence rate of syphilis (21 : 100,000, while a significantly higher prevalence was found among immigrants from Africa, Eastern Europe, and South America (odds ratios of 19.0, 10.8, and 7.3, resp., P<0.001 for each. About 33.2% of the seropositive donors had evidence of recent infection, and 66.8% had past infections. Incidence rate reached 8 : 100,000 person-years. Coinfection with HIV, HCV, and HBV was calculated as 8%, 1.88%, and 0.37% for positive donations, respectively. The data support the need to continue screening blood donors in Israel for syphilis and employ preventive measures to populations at risk, in order to improve public health, blood safety, and quality. A subsequent study to assess blood donors’ knowledge, attitude, and behavior is planned. In times of global migration this information may be useful to blood services worldwide.

  9. Recovery of Unrelated Donors of Peripheral Blood Stem Cells versus Recovery of Unrelated Donors of Bone Marrow: A Prespecified Analysis from the Phase III Blood and Marrow Transplant Clinical Trials Network Protocol 0201.

    Science.gov (United States)

    Burns, Linda J; Logan, Brent R; Chitphakdithai, Pintip; Miller, John P; Drexler, Rebecca; Spellman, Stephen; Switzer, Galen E; Wingard, John R; Anasetti, Claudio; Confer, Dennis L

    2016-06-01

    We report a comparison of time to recovery, side effects, and change in blood counts from baseline to after donation from unrelated donors who participated in the Blood and Marrow Transplant Clinical Trials Network phase III randomized, multicenter trial (0201) in which donor-recipient pairs were randomized to either peripheral blood stem cell (PBSC) or bone marrow (BM) donation. Of the entire cohort, 262 donated PBSC and 264 donated BM; 372 (71%) donors were from domestic and 154 (29%) were from international centers (145 German and 9 Canadian). PBSC donors recovered in less time, with a median time to recovery of 1 week compared with 2.3 weeks for BM donors. The number of donors reporting full recovery was significantly greater for donors of PBSC than of BM at 1, 2, and 3 weeks and 3 months after donation. Multivariate analysis showed that PBSC donors were more likely to recover at any time after donation compared with BM donors (hazard ratio, 2.08; 95% confidence interval [CI], 1.73 to 2.50; P ratio, 1.13; 95% CI, .74 to 1.74; P = .556). Blood counts were affected by product donated, with greater mean change from baseline to after donation for white blood cells, neutrophils, mononuclear cells, and platelets in PBSC donors whereas BM donors experienced a greater mean change in hemoglobin. This analysis provided an enhanced understanding of donor events as product donated was independent of physician bias or donor preference. PMID:27013014

  10. [Immunoblot as a supplemental test to detect antibodies to hepatitis C virus in blood donors].

    Science.gov (United States)

    Souto, Francisco José Dutra; Ribeiro, Luciano Côrrea; Perazolo, Gustavo Faria; Fortes, Hildenete Monteiro; Saldanha, Alzira Almeida

    2002-01-01

    Supplemental tests using Immunoblot are recommended to improve specificity of anti-HCV by ELISA. In Brazil immunoblot is not officially recommended. Aiming to identify EIA false-positive rate 70 positive EIA anti-HCV blood donors were submitted to 3rd generation immunoblot at Hemocentro of Mato Grosso State where polymerase chain reaction tests are not performed. There were 44 (62.9%) immunoblot-positive, 22 (31.4%) negative and 4 (5.7%) indeterminate. Anti-HCV immunoblot can distinguish blood donors with false-positive ELISA from those who need medical assessment. Our data suggest that immunoblot could be useful in Brazilian blood banks where molecular biology tests are not available. PMID:11873265

  11. Significance of isolated hepatitis B core antibody in blood donors from São Paulo

    Directory of Open Access Journals (Sweden)

    ALMEIDA NETO Cesar de

    2001-01-01

    Full Text Available The clinical significance of isolated anti-HBc is still a challenge. To elucidate the real importance of this finding in our blood donors, an investigation algorithm was tested. One hundred and twelve isolated anti-HBc seropositive blood donors underwent clinical evaluation and retesting of HBV markers. Those who presented repeatedly reactive isolated anti-HBc, received a single dose of hepatitis B recombinant vaccine to verify anti-HBs early response. A HBV-DNA determination by PCR was done for those who did not test positive to anti-HBs after vaccine. The level of anti-HBc was recorded as a ratio of the sample-to-cut-off values (S:C ratio in 57 candidates at donation. Comparing true and false-positive anti-HBc results, the different S:C ratios of them were statistically significant and when less than 2, implying in a false-positive result probability over 80%. A high percent of false-positive results (16.07% was verified after anti-HBc retesting. HBV immunity was characterized in 49.11%, either by anti-HBs detection in retesting (15.18%, or after a single dose HBV vaccination (33.93%. HBV-DNA was negative in all tested donors. In conclusion, this algorithm was useful to clarify the meaning of isolated anti-HBc in most of our blood donors.

  12. Hepatitis B core antibody testing in Indian blood donors: A double-edged sword!

    Directory of Open Access Journals (Sweden)

    R N Makroo

    2012-01-01

    Full Text Available Background: Until lately, anti-HBc antibodies were considered an effective marker for occult Hepatitis B virus (HBV infection and have served their role in improving blood safety. But, with the development of advanced tests for HBV DNA detection, the role of anti-HBc in this regard stands uncertain. Materials and Methods: Anti-HBc and HBsAg ELISA and ID-NAT tests were run in parallel on donor blood samples between April 1, 2006 and December 31, 2010 at the Department of Transfusion Medicine, Indraprastha Apollo Hospitals, New Delhi. A positive ID-NAT was followed by Discriminatory NAT assay. Results: A total of 94 247 samples were tested with a total core positivity rate of 10.22%. We identified nearly 9.17% of donors who were reactive for anti-HBc and negative for HBsAg and HBV DNA. These are the donors who are potentially non-infectious and may be returned to the donor pool. Conclusion: Although anti HBc testing has a definite role in improving blood safety, centers that have incorporated NAT testing may not derive any additional benefit by performing anti-HBc testing, especially in resource-limited countries like ours.

  13. High frequencies of HGV and TTV infections in blood donors in Hangzhou

    Institute of Scientific and Technical Information of China (English)

    Jie Yan; Li-Li Chen; Yi-Hui LUO; Ya-Fei Mao; Meng He

    2001-01-01

    ALM To determine the frequencies of HGV and TTVinfections in blood donors in Hangzhou.METHODS RT-nested PCR for HGV RNA detection and semi-nested PCR for TTV DNA detection in the sera from 203 blood donors, and nucleotide sequence analysis were performed.``RESULTS Thirty-two ( 15.8%) and 30 (14.8%) of the 203serum samples were positive for HGV RNA and TTV DNA,respectively. And 5 (2.5%) of the 203 serum samples were detectable for both HGV RNA and TTV DNA.Homology of the nucleotide sequences of HGV RT-nested PCR products and TTV semi-nested PCR products from 3serum samples compared with the reported HGV and TTV sequences was 89.36%. 87.94%, 88.65% and 63.51%.65.77% and 67.12%. respectively.``CONCLUSION The infection rates of HGV and/or TTV inblood donors are relatively high. and to establish HGV and TTV examinations to screen blood donors is needed for transfusion security. The genomic heterogeneity of TTV or HGV is present in the isolates from different areas.``

  14. Genetic factors influencing ferritin levels in 14,126 blood donors

    DEFF Research Database (Denmark)

    Sørensen, Erik; Rigas, Andreas S; Thørner, Lise W;

    2015-01-01

    AND METHODS: The effect of six single-nucleotide polymorphisms (SNPs) on ferritin levels in 14,126 blood donors were investigated in four genes: in Human Hemochromatosis Protein gene (HFE; rs1800562 and rs179945); in Transmembrane Protease gene, Serine 6 (TMPRSS6-regulating hepcidin; rs855791); in BTB domain...... with iron deficiency in women. Results for all other genetic variants were insignificant. CONCLUSION: Genetic variants associated with hemochromatosis may protect donors against depleted iron stores. In addition, we showed that presence of the T-allele at rs855791 in TMPRSS6 was associated with lower iron...

  15. About hematopoietic properties of peripheral blood lymphocytes RNA from patients with polycythemia vera and healthy donors

    Directory of Open Access Journals (Sweden)

    A. G. Babaeva

    2015-06-01

    Full Text Available Total RNA isolated from peripheral blood lymphocytes of donor and patient with polycythemia, stimulates hematopoiesis in rats with toxic aplastic anemia due to benzene administration. Total RNA of lymphocytes from polycythemia patient has a more pronounced effect on the erythroid, myeloid and megakaryocytic hematopoiesis comparing to total RNA from donor lymphocytes. The greatest stimulatory effectof RNA observed after 21 days from the start of experiment. Total RNA of lymphocytes from polycythemia patient largely activates erythropoiesis promoting restoration of reticulocyte count in animals with aplastic anemia.

  16. About hematopoietic properties of peripheral blood lymphocytes RNA from patients with polycythemia vera and healthy donors

    Directory of Open Access Journals (Sweden)

    A. G. Babaeva

    2015-01-01

    Full Text Available Total RNA isolated from peripheral blood lymphocytes of donor and patient with polycythemia, stimulates hematopoiesis in rats with toxic aplastic anemia due to benzene administration. Total RNA of lymphocytes from polycythemia patient has a more pronounced effect on the erythroid, myeloid and megakaryocytic hematopoiesis comparing to total RNA from donor lymphocytes. The greatest stimulatory effectof RNA observed after 21 days from the start of experiment. Total RNA of lymphocytes from polycythemia patient largely activates erythropoiesis promoting restoration of reticulocyte count in animals with aplastic anemia.

  17. Blood donor deferral: time for change? An evidence-based analysis

    OpenAIRE

    Borra V; Vandewalle G; Van Remoortel H; Compernolle V; De Buck E; Vandekerckhove P

    2016-01-01

    Vere Borra,1 Giovani Vandewalle,1 Hans Van Remoortel,1 Veerle Compernolle,1,2 Emmy De Buck,1 Philippe Vandekerckhove1–31Belgian Red Cross-Flanders, Mechelen, 2Faculty of Medicine, University of Ghent, Ghent, 3Department of Public Health and Primary Care, Faculty of Medicine, KU Leuven, Leuven, BelgiumAbstract: Donor selection remains an important part in the safety of the blood supply all over the world. Yet, donor deferral criteria seem to be strongly based on the precautionary princip...

  18. Blood donor deferral: time for change? An evidence-based analysis

    OpenAIRE

    Vandekerckhove, Philippe; Borra,Vere; Vandewalle,Giovani; van Remoortel, Hans; Compernolle, Veerle; De Buck, Emmy

    2016-01-01

    Vere Borra,1 Giovani Vandewalle,1 Hans Van Remoortel,1 Veerle Compernolle,1,2 Emmy De Buck,1 Philippe Vandekerckhove1–31Belgian Red Cross-Flanders, Mechelen, 2Faculty of Medicine, University of Ghent, Ghent, 3Department of Public Health and Primary Care, Faculty of Medicine, KU Leuven, Leuven, BelgiumAbstract: Donor selection remains an important part in the safety of the blood supply all over the world. Yet, donor deferral criteria seem to be strongly based on the precautionary pri...

  19. OCCULT HEPATITIS B VIRUS INFECTION AMONG BLOOD DONORS WITH ANTIBODIES TO HEPATITIS B CORE ANTIGEN

    Directory of Open Access Journals (Sweden)

    A. Jafarzadeh

    2008-04-01

    Full Text Available Diagnosis of hepatitis B is routinely based on of serological assay of hepatitis B surface antigen (HBsAg. Occult hepatitis B virus (HBV infection is generally defined as the detection of HBV -DNA in the serum or tissues of subjects who have negative test for HBsAg. Transmission of HBV infection has been documented from HBsAg negative, anti-HBc positive blood and organ donors. The aim of this study was to determine the rate of occult HBV infection among HBsAg negative and anti-HBc positive blood donors of Rafsanjan blood transfusion center. ‎ Sera from 270 healthy blood donors who were negative for both HBsAg and anti-HCV, were tested for anti-HBc antibodies by use of ELISA technique. The samples that were negative for HBsAg but positive for anti-HBc markers also examined for the presence of HBV-DNA by polymerase chain reaction (PCR. ‎ Out of 270 HBsAg negative blood samples, 14 samples (5.18% were positive for anti-HBc antibodies. HBV-DNA was detected in 4/14 (28.57% of HBsAg negative and anti-HBc positive samples. Moreover, anti-HBs antibody was detected in 2/4 (50% of HBV-DNA positive samples. ‎ These results indicated that HBV-DNA found in the majority of HBsAg negative and anti-HBc-positive donors. In addition, the present study recommend the incorporation of routine anti-HBc screening of blood as a surrogate marker of occult HBV infection to prevent some transfusion-transmitted HBV infections.

  20. Anti-HBc screening in Indian blood donors: Still an unresolved issue

    Institute of Scientific and Technical Information of China (English)

    Hari Krishan Dhawan; Neelam Marwaha; Ratti Ram Sharma; Yogesh Chawla; Beenu Thakral; Karan Saluja; Sanjeev Kumar Sharma; Manish K Thakur; Ashish Jain

    2008-01-01

    AIM:To study the seroprevalence of antibody to hepatitis B core antigen (anti-HBc) in healthy blood donors negative for HBsAg and to evaluate whether anti-HBc detection could be adopted in India as a screening assay for HBV in addition to HBsAg.METHODS:A total of 1700 serum samples collected from HBsAg-negative healthy blood donors were tested for the presence of anti-HBc antibody (IgM + IgG).All samples reactive for anti-HBc antibody were then investigated for presence of anti-HBs and for liver function tests (LFTs).One hundred serum samples reactive for anti-HBc were tested for HBV DNA by PCR method.RESULTS:Out of 1700 samples tested,142 (8.4%)blood samples were found to be reactive for anti-HBc.It was significantly lower in voluntary (6.9%) as compared to replacement donors (10.4%,P=0.011).Seventytwo (50.7%) anti-HBc reactive samples were also reactive for anti-HBs with levels >10 mIU/mL and 70(49.3%) samples were non-reactive for anti-HBs,these units were labeled as anti-HBc-only.These 142 anti-HBc reactive units were also tested for liver function test.HBV DNA was detected in only 1 of 100 samples tested.CONCLUSION:Keeping in view that 8%-18% of donor population in India is anti-HBc reactive,inclusion of anti-HBc testing will lead to high discard rate.Anti-HBs as proposed previously does not seem to predict clearance of the virus.Cost effectiveness of introducing universal anti-HBc screening and discarding large number of blood units versus considering ID NAT (Individual donor nuclic acid testing) needs to be assessed.

  1. A study on patterns of co-infections among blood donors at the blood bank of a tertiary care referral teaching hospital in South India

    OpenAIRE

    Suresh B; Sreedhar Babu KV; Chandra Mouli P; Jothibai DS

    2016-01-01

    Background: Blood serves as a vehicle for transmission of blood-borne pathogens including human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), malaria parasite (MP) and syphilis. Safe blood and blood products should be transfused to all patients in need for blood transfusion. Material and Methods: All blood donors attending to the blood bank during the period January 2009 to December 2014 were screened for hepatitis B surface antigen (HBsAg), anti HCV antib...

  2. Significance of tests for HBeAg and anti-HBe in HBsAg positive blood donors.

    OpenAIRE

    Dow, B C; Macvarish, I; Barr, A.; Crawford, R J; Mitchell, R.

    1980-01-01

    A sensitive radioimmunoassay method for HBeAg and anti-HBe allowed 98.4% of HBsAg positive blood donors to be classified. HBeAg was more prevalent in blood donors under 30 years of age and anti-HBe was more prevalent in those over 30 years. The mean HBsAg concentration was four times greater in donors with HBeAg than in those with anti-HBe. No significant associations were found with sex, blood groups, or HBsAg subtypes. It is likely that this test system will be extremely useful to blood tra...

  3. TT virus and hepatitis G virus infections in Korean blood donors and patients with chronic liver disease

    Institute of Scientific and Technical Information of China (English)

    Mee Juhng Jeon; Jong Hee Shin; Soon Pal Suh; Yong Chai Lim; Dong Wook Ryang

    2003-01-01

    AIM: To determine the prevalences of TTV and HGV infections among blood donors and patients with chronic liver disease in Korea, to investigate the association of TTV and HGV infections with blood transfusion, and to assess the correlation between TTV and HGV viremia and hepatic damage.METHODS: A total of 391 serum samples were examined in this study. Samples were obtained from healthy blood donors (n= 110), hepatitis B surface antigen (HBsAg)-positive donors (n=112), anti-hepatitis C virus (anti-HCV)-positive donors (n=69), patients with type B chronic liver disease (n=81), and patients with type C chronic liver disease (n= 19).TTV DNA was detected using the hemi-nested PCR. HGV RNA was tested using RT-PCR. A history of blood transfusion and serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were also determined.RESULTS: TTV DNA was detected in 8.2 % of healthy blood donors, 16.1% of HBsAg-positive donors, 20.3 % of antiHCV-positive donors, 21.0 % of patients with type B chronic liver disease, and 21.1% of patients with type C chronic liver disease. HGV RNA was detected in 1.8 % of healthy blood donors, 1.8 % of HBsAg-positive donors, 17.4 % of anti-HCV-positive donors, 13.6% of patients with type B chronic liver disease, and 10.5% of patients with type C chronic liver disease. The prevalence of TTV and HGV infections in HBV- or HCV-positive donors and patients was significantly higher than in healthy blood donors (P<0.05),except for the detection rate of HGV in HBsAg-positive donors which was the same as for healthy donors. There was a history of transfusion in 66.7% of TTV DNA-positive patients and 76.9% of HGV RNA-positive patients (P<0.05). No significant increase in serum ALT and AST was detected in the TTV- or HGV-positive donors and patients.CONCLUSION: TTV and HGV infections are more frequently found in donors and patients infected with HBV or HCV than in healthy blood donors. However, there is no significant

  4. High prevalence of HIV p24 antigen among HIV antibody negative prospective blood donors in Ile-Ife, Nigeria.

    Science.gov (United States)

    Japhet, Margaret Oluwatoyin; Adewumi, Moses Olubusuyi; Adesina, Olufisayo Adeyemi; Donbraye, Emmanuel

    2016-01-01

    Blood transfusion service centers in Nigeria screen donated blood for markers of HIV infection using antibody- (Ab) based rapid test and in some centers, positives are re-tested using Ab-based ELISA. Paucity of data exists on p24 antigen prevalence among HIV Ab-negative donors in Nigeria. This study aims at detecting HIV p24 antigen among prospective blood donors in Osun State, Nigeria. Prospective blood donors negative for HIV antibodies using Determine test kit were re-tested using BIORAD GENSCREEN Ultra Ag-Ab ELISA kit, a fourth-generation ELISA kit that detects HIV antibodies/p24 antigen. Of the 169 HIV Ab-negative prospective donors, 10 (5.9%) were positive for HIV p24 antigen and 70% (7/10) of them were in the age range 18-30 years. Results of this study show that blood transfusion is still one of the major routes of HIV transmission in Nigeria and a higher proportion is among youth. Inclusion of p24 antigen testing into the blood donor screening will help reduce transfusion associated HIV in Nigeria if Nucleic Acid Testing (NAT) of all blood donor samples is not affordable; also, HIV enlightenment programs tailored toward youth may help reduce this rate among donors since more young people donate blood in low/middle-income countries than in high-income countries. PMID:27049173

  5. Clinical and epidemiological profile of female blood donors with positive serology for viral hepatitis B

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    Janaína Luz Narciso-Schiavon

    2015-10-01

    Full Text Available ABSTRACTINTRODUCTION:Since women are frequently the minority among blood donors worldwide, studies evaluating this population usually reflect male features. We assessed the features of female blood donors with positive serology for HBV and compared them with those of men.METHODS The study comprised consecutive blood donors referred to a specialized liver disease center to be evaluated due to HBsAg- and/or anti-HBc-positive tests.RESULTS: The study encompassed 1,273 individuals, 219 (17.2% of whom were referred due to positive HBsAg test and 1,054 (82.8% due to reactive anti-HBc test. Subjects' mean age was 36.8±10.9 years, and 28.7% were women. Female blood donors referred for positive HBsAg screening tests demonstrated higher prevalence of healthcare workers (9.3% vs 2.5% and lower prevalence of sexual risk behaviors (15.1% vs 41.1% and alcohol abuse (1.9% vs 19.8% compared to men. Women had lower ALT (0.6 vs 0.8×ULN, AST (0.6 vs 0.8×ULN, direct bilirubin (0.2 vs 0.3mg/dL, and alkaline phosphatase (0.5 vs 0.6×ULN levels and higher platelet count (223,380±50,293 vs 195,020±53,060/mm3. Women also had a higher prevalence of false-positive results (29.6% vs 17.0%. No differences were observed with respect to liver biopsies. Female blood donors referenced for reactive anti-HBc screening tests presented similar clinical, epidemiological, and biochemical characteristics to those reported for positive HBsAg screening tests and similarly had a higher prevalence of false-reactive results.CONCLUSIONS: Compared to men, female blood donors with positive HBsAg and/or anti-HBc screening tests demonstrated higher prevalence of professional risk and false-positive results and reduced alteration of liver chemistry.

  6. Hepatitis B, HIV, and Syphilis Seroprevalence in Pregnant Women and Blood Donors in Cameroon.

    Science.gov (United States)

    Dionne-Odom, Jodie; Mbah, Rahel; Rembert, Nicole J; Tancho, Samuel; Halle-Ekane, Gregory E; Enah, Comfort; Welty, Thomas K; Tih, Pius M; Tita, Alan T N

    2016-01-01

    Objectives. We estimated seroprevalence and correlates of selected infections in pregnant women and blood donors in a resource-limited setting. Methods. We performed a cross-sectional analysis of laboratory seroprevalence data from pregnant women and voluntary blood donors from facilities in Cameroon in 2014. Rapid tests were performed to detect hepatitis B surface antigen, syphilis treponemal antibodies, and HIV-1/2 antibodies. Blood donations were also tested for hepatitis C and malaria. Results. The seroprevalence rates and ranges among 7069 pregnant women were hepatitis B 4.4% (1.1-9.6%), HIV 6% (3.0-10.2%), and syphilis 1.7% (1.3-3.8%) with significant variability among the sites. Correlates of infection in pregnancy in adjusted regression models included urban residence for hepatitis B (aOR 2.9, CI 1.6-5.4) and HIV (aOR 3.5, CI 1.9-6.7). Blood donor seroprevalence rates and ranges were hepatitis B 6.8% (5.0-8.8%), HIV 2.2% (1.4-2.8%), syphilis 4% (3.3-4.5%), malaria 1.9%, and hepatitis C 1.7% (0.5-2.5%). Conclusions. Hepatitis B, HIV, and syphilis infections are common among pregnant women and blood donors in Cameroon with higher rates in urban areas. Future interventions to reduce vertical transmission should include universal screening for these infections early in pregnancy and provision of effective prevention tools including the birth dose of univalent hepatitis B vaccine. PMID:27578957

  7. Hepatitis B, HIV, and Syphilis Seroprevalence in Pregnant Women and Blood Donors in Cameroon

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    Jodie Dionne-Odom

    2016-01-01

    Full Text Available Objectives. We estimated seroprevalence and correlates of selected infections in pregnant women and blood donors in a resource-limited setting. Methods. We performed a cross-sectional analysis of laboratory seroprevalence data from pregnant women and voluntary blood donors from facilities in Cameroon in 2014. Rapid tests were performed to detect hepatitis B surface antigen, syphilis treponemal antibodies, and HIV-1/2 antibodies. Blood donations were also tested for hepatitis C and malaria. Results. The seroprevalence rates and ranges among 7069 pregnant women were hepatitis B 4.4% (1.1–9.6%, HIV 6% (3.0–10.2%, and syphilis 1.7% (1.3–3.8% with significant variability among the sites. Correlates of infection in pregnancy in adjusted regression models included urban residence for hepatitis B (aOR 2.9, CI 1.6–5.4 and HIV (aOR 3.5, CI 1.9–6.7. Blood donor seroprevalence rates and ranges were hepatitis B 6.8% (5.0–8.8%, HIV 2.2% (1.4–2.8%, syphilis 4% (3.3–4.5%, malaria 1.9%, and hepatitis C 1.7% (0.5–2.5%. Conclusions. Hepatitis B, HIV, and syphilis infections are common among pregnant women and blood donors in Cameroon with higher rates in urban areas. Future interventions to reduce vertical transmission should include universal screening for these infections early in pregnancy and provision of effective prevention tools including the birth dose of univalent hepatitis B vaccine.

  8. Hepatitis B, HIV, and Syphilis Seroprevalence in Pregnant Women and Blood Donors in Cameroon

    Science.gov (United States)

    Mbah, Rahel; Rembert, Nicole J.; Tancho, Samuel; Halle-Ekane, Gregory E.; Enah, Comfort; Welty, Thomas K.; Tih, Pius M.; Tita, Alan T. N.

    2016-01-01

    Objectives. We estimated seroprevalence and correlates of selected infections in pregnant women and blood donors in a resource-limited setting. Methods. We performed a cross-sectional analysis of laboratory seroprevalence data from pregnant women and voluntary blood donors from facilities in Cameroon in 2014. Rapid tests were performed to detect hepatitis B surface antigen, syphilis treponemal antibodies, and HIV-1/2 antibodies. Blood donations were also tested for hepatitis C and malaria. Results. The seroprevalence rates and ranges among 7069 pregnant women were hepatitis B 4.4% (1.1–9.6%), HIV 6% (3.0–10.2%), and syphilis 1.7% (1.3–3.8%) with significant variability among the sites. Correlates of infection in pregnancy in adjusted regression models included urban residence for hepatitis B (aOR 2.9, CI 1.6–5.4) and HIV (aOR 3.5, CI 1.9–6.7). Blood donor seroprevalence rates and ranges were hepatitis B 6.8% (5.0–8.8%), HIV 2.2% (1.4–2.8%), syphilis 4% (3.3–4.5%), malaria 1.9%, and hepatitis C 1.7% (0.5–2.5%). Conclusions. Hepatitis B, HIV, and syphilis infections are common among pregnant women and blood donors in Cameroon with higher rates in urban areas. Future interventions to reduce vertical transmission should include universal screening for these infections early in pregnancy and provision of effective prevention tools including the birth dose of univalent hepatitis B vaccine.

  9. Assessing ABO/Rh Blood Group Frequency and Association with Asymptomatic Malaria among Blood Donors Attending Arba Minch Blood Bank, South Ethiopia.

    Science.gov (United States)

    Alemu, Getaneh; Mama, Mohammedaman

    2016-01-01

    Background. Determination of the various ABO/Rh blood group distributions and their association with malaria infection has paramount importance in the context of transfusion medicine and malaria control. Methods. Facility based cross-sectional study was conducted from February to June, 2015, to assess ABO/Rh blood groups distribution and their association with asymptomatic malaria. A structured questionnaire was used to collect data. Blood grouping was done using monoclonal antibodies. Thin and thick blood films were examined for Plasmodium parasites. Data were analyzed using SPSS version 20.0. Results. A total of 416 blood donors participated with median age of 22 ± 0.29 (median ± standard error of the mean). Distribution of ABO phenotypes, in decreasing order, was O (175, 42.1%), A (136, 32.7%), B (87, 20.9%), and AB (18, 4.3%). Most of them were Rh+ (386, 92.8%). The overall malaria prevalence was 4.1% (17/416). ABO blood group is significantly associated with malaria infection (P = 0.022). High rate of parasitemia was seen in blood group O donors (6.899, P = 0.003) compared to those with other ABO blood groups. Conclusion. Blood groups O and AB phenotypes are the most and the least ABO blood groups, respectively. There is significant association between ABO blood group and asymptomatic malaria parasitemia.

  10. Assessing ABO/Rh Blood Group Frequency and Association with Asymptomatic Malaria among Blood Donors Attending Arba Minch Blood Bank, South Ethiopia.

    Science.gov (United States)

    Alemu, Getaneh; Mama, Mohammedaman

    2016-01-01

    Background. Determination of the various ABO/Rh blood group distributions and their association with malaria infection has paramount importance in the context of transfusion medicine and malaria control. Methods. Facility based cross-sectional study was conducted from February to June, 2015, to assess ABO/Rh blood groups distribution and their association with asymptomatic malaria. A structured questionnaire was used to collect data. Blood grouping was done using monoclonal antibodies. Thin and thick blood films were examined for Plasmodium parasites. Data were analyzed using SPSS version 20.0. Results. A total of 416 blood donors participated with median age of 22 ± 0.29 (median ± standard error of the mean). Distribution of ABO phenotypes, in decreasing order, was O (175, 42.1%), A (136, 32.7%), B (87, 20.9%), and AB (18, 4.3%). Most of them were Rh+ (386, 92.8%). The overall malaria prevalence was 4.1% (17/416). ABO blood group is significantly associated with malaria infection (P = 0.022). High rate of parasitemia was seen in blood group O donors (6.899, P = 0.003) compared to those with other ABO blood groups. Conclusion. Blood groups O and AB phenotypes are the most and the least ABO blood groups, respectively. There is significant association between ABO blood group and asymptomatic malaria parasitemia. PMID:26925291

  11. Occult hepatitis B virus infection among blood donors from the Brazilian Amazon: implications for transfusion policy

    Science.gov (United States)

    Moresco, M. N. dos S.; Virgolino, H. de A.; de Morais, M. P. E.; da Motta-Passos, I.; Gomes-Gouvêa, M. S.; de Assis, L. M. S.; Aguiar, K. R. de L.; Lombardi, S. C. F.; Malheiro, A.; Cavalheiro, N. de P.; Levi, J. E.; Torres, K. L.

    2016-01-01

    Background Brazil requires the performance of both a test for hepatitis B surface antigen (HBsAg) and a test for antibodies to the core of hepatitis B for blood donor screening. Blood centres in regions of high HBV endemicity struggle to maintain adequate stocks in face of the high discard rates due to anti-HBc reactivity. We evaluated the potential infectivity of donations positive for anti-HBc in search of a rational approach for the handling of these collections. Study Design and Methods We tested anti-HBc reactive blood donations from the state of Amazonas for the presence of HBV DNA and for titres of anti-HBs. The study population consists of village-based donors from the interior of Amazonas state. Results Among 3600 donations, 799 were anti-HBc reactive (22·2%). We were able to perform real-time PCR for the HBV S gene on specimens from 291 of these donors. Eight of these samples were negative for HBsAg and positive for HBV DNA and were defined as occult B virus infections (2·7%). Six of those eight specimens had anti-HBs titres above 100 mIU/ml, indicating the concomitant presence of the virus with high antibody titres. Conclusion A small proportion of anti-HBc reactive donors carry HBV DNA and anti-HBs testing is not useful for predicting viremia on them. This finding indicates the possibility of HBV transmission from asymptomatic donors, especially in areas of high HBV prevalence. Sensitive HBV DNA nucleic acid testing may provide another level of safety, allowing eventual use of anti-HBc reactive units in critical situations. PMID:24697276

  12. Pre-donation deferral of blood donors in South Indian set-up: An analysis

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    Sundar P

    2010-01-01

    Full Text Available Background: It is well known that quite a large number of apparently healthy donors are not able to donate blood successfully because of varied reasons. Aim: We want to analyze the rate and various reasons for deferrals. Materials and Methods: A retrospective analysis of records of the donors, for 3 years, from January 2005 to December 2007 was done, in order to find out the rate and causes of deferral in four categories of age groups, both in male and female, in our Transfusion Medicine Centre, Bangalore, India. Result: There were 16,706 donors, of which 976 donors were deferred (5.84% for various reasons. Of the 16,706 donors registered for donation, females constituted only 11.27%. And deferral rate was about five times more for female (19.85% compared to male (4.06%. The three most common reasons for deferral in female were low hemoglobin levels, low body weight, and hypotension. The deferral rate was higher in the age group of 18-25 years and most common cause was low hemoglobin level. In male, the three most common reasons for deferral were hypertension, under weight, and low hemoglobin levels. The deferral rate varied from 4 to 15% as reported in the literature. The most common cause of deferral in our study and in several studies available in the literature is the same.

  13. SALMON SOFT ROE DNA ON BLOOD CELLS SECRETION OF CYTOKINES IN HEALTHY DONORS

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    L. N. Fedjanina

    2005-01-01

    Full Text Available Abstract. Salmon soft roe DNA influence on healthy donors blood cells secretion of early hemopoietic factors (IL-3, GM-CSF, TNFα as well as biologically active substance influence on cytokine balance of Тh1 and Тh2 responses (IFNγ, IL-10 in vitro was studied. It is established, that DNA has modulatory effect on secretion of all investigated cytokines - IL-3, GM-CSF, TNFα, INFγ and IL-10 by blood cells of healthy donors, increases their initially low concentration, reduces initially high and does not have essential influence at an average level of their secretion. Under action of DNA IFNγ level (stimulation index=3,3 increases more significantly than IL-10 level (stimulation index =1,9. Thus, salmon soft roe DNA possesses immunomodulatory properties.

  14. Occult Hepatitis B virus infection in previously screened, blood donors in Ile-Ife, Nigeria: implications for blood transfusion and stem cell transplantation

    OpenAIRE

    Amadin A. Olotu; Oyelese, Adesola O.; Salawu, Lateef; Rosemary A. Audu; Azuka P. Okwuraiwe; Aboderin, Aaron O.

    2016-01-01

    Background Hepatitis B virus (HBV) transmission through blood transfusion is reduced by screening for hepatitis B surface antigen (HBsAg). However this method cannot detect the presence of occult hepatitis B virus infection. This study sought to determine the prevalence of occult hepatitis B virus infection among blood donors in Ile-Ife, Nigeria. For the first time in Nigeria we employed an automated real-time PCR- method to investigate the prevalence of occult HBV in blood donors. Methods Bl...

  15. Skin Recurrence of Transformed Mycosis Fungoides Postumbilical Cord Blood Transplant despite Complete Donor Chimerism

    OpenAIRE

    Rahul Pawar; Anup Kasi Loknath Kumar; Janet Woodroof; Wei Cui; Joseph McGuirk; Sunil Abhyankar; Sid Ganguly; Anurag Singh; Tara Lin; Omar Aljitawi

    2014-01-01

    Background. Allogeneic stem cell transplant is the treatment of choice for systemic cutaneous T-cell lymphoma (CTCL) which provides graft-versus-lymphoma effect. Herein we discuss a case of recurrence of CTCL skin lesions after cord blood transplant in a patient who continued to have 100% donor chimerism in bone marrow. Case Presentation. A 48-year-old female with history of mycosis fungoides (MF) presented with biopsy proven large cell transformation of MF. PET scan revealed multiple adenop...

  16. Estimating the risk of parvovirus B19 infection in blood donors and pregnant women in Japan

    OpenAIRE

    難波江, 功二

    2014-01-01

    主査: 西脇祐司 /タイトル: Estimating the risk of parvovirus B19 infection in blood donors and pregnant women in Japan /著者: Koji Nabae, Hiroshi Satoh, Hiroshi Nishiura, Keiko Tanaka-Taya,Nobuhiko Okabe, Kazunori Oishi, Kunichika Matsumoto, Tomonori Hasegawa /掲載誌: PLoS ONE /巻号・発行年等: 9(3): e92519, 2014 /

  17. CYP11B2 gene polymorphism among coronary heart disease patients and blood donors in Malaysia.

    Science.gov (United States)

    Normaznah, Y; Azizah, M R; Kuak, S H; Rosli, M A

    2015-04-01

    Various previous studies have reported the implication of CYP11B2 gene polymorphism in the pathophysiology of cardiovascular diseases. In particular, the -344T/C polymorphism, which is located at a putative binding site for the steroidogenic transcription factor (SF-1) has been associated with essential hypertension, left ventricular dilation and coronary heart disease. In the present study, we aim to determine the allele and genotype frequencies of the CYP11B2 gene in patients with clinical manifestation of coronary heart disease and confirmed by angiography and blood donors and to calculate the association of the gene polymorphism with CHD. A total of 79 DNA from patients with coronary heart disease admitted to the National Heart Institute and 84 healthy blood donors have been genotyped using polymerase chain reaction technique followed by restriction enzyme digestion (RFLP). Results of the study demonstrated that out of 79 for the patients, 40 were homozygous T, 10 were homozygous C and 29 were heterozygous TC. The frequencies of genotype TT, CC and TC for patients were 0.5, 0.13 and 0.36 respectively. The frequencies of allele T and C in patients were 0.68 and 0.31 respectively. While for the blood donors, 40 subjects were of homozygous T, 7 were homozygous C and 37 were heterozygous TC. The genotype frequencies for the TT, CC and TC were 0.47, 0.08 and 0.44 respectively. The frequency of the allele T was 0.69 and allele C was 0.3. Chi-Square analysis showed that there was no significant difference in the genotype and C allele frequencies between the CHD patients and the blood donors. Our study suggests that there is lack of association between -344T/C polymorphism of CYP11B2 gene and coronary heart disease.

  18. Adverse effects to transfusion with red donor blood cells are frequent

    DEFF Research Database (Denmark)

    Pommergaard, Hans-Christian; Nørgaard, Astrid; Burcharth, Jakob;

    2014-01-01

    Adverse effects to transfusion with red donor blood cells are potentially life-threatening. Due to screening, transmission of infectious diseases has decreased; however, the risk is still present. Various immune reactions are common including simple allergic reactions as well as devastating...... conditions such as transfusion-related acute lung injury and circulatory overload in patients with heart disease. Knowledge of the clinical signs of transfusion-related complications is important for clinicians in order to provide the best possible treatment....

  19. Immunity to diphtheria and tetanus among blood donors in Arak, central province of Iran.

    OpenAIRE

    Ali Eslamifar; Amitis Ramezani; Mohammad Banifazl; Masoomeh Sofian; Fatemeh-Alsadat Mahdaviani; Farhad Yaghmaie; Arezoo Aghakhani

    2014-01-01

    Background and Objectives Tetanus and diphtheria are vaccine-preventable, infectious diseases with significant morbidity and mortality. Immunization by the diphtheria and tetanus toxoid (DT) has been applied in Iran for almost 50 years. However, there are very few data about the rate of immunity to these diseases in the adult population. the humoral immunity to tetanus and diphtheria among blood donors in Arak city, central provice of Iran were investigated. Patients & Methods A total of 530 ...

  20. Estimating the risk of parvovirus B19 infection in blood donors and pregnant women in Japan.

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    Koji Nabae

    Full Text Available BACKGROUND: Seroepidemiological study of parvovirus B19 has not taken place for some 20 years in Japan. To estimate the risk of parvovirus B19 infection in Japan among blood donors and pregnant women in this century, a seroepidemiological survey and statistical modeling of the force of infection were conducted. METHODOLOGY/PRINCIPAL FINDINGS: The time- and age-specific seroprevalence data were suggestive of strong age-dependency in the risk of infection. Employing a piecewise constant model, the highest forces of infection of 0.05 and 0.12 per year were observed among those aged 0-4 and 5-9 years, respectively, while estimates among older individuals were less than 0.01 per year. Analyzing the antigen detection data among blood donors, the age-specific proportion positive was highest among those aged 30-39 years, agreeing with the presence of dip in seroprevalence in this age-group. Among pregnant women, up to 107 fetal deaths and 21 hydrops fetalis were estimated to have occurred annually across Japan. CONCLUSIONS: Seroepidemiological profiles of PVB19 infection in Japan was characterized with particular emphasis on the risk of infection in blood donors and the burden of infection among pregnant women. When a vaccine becomes available in the future, a similar seroepidemiological study is expected to play a key role in planning the appropriate immunization policy.

  1. Characteristics of Lapsed German Whole Blood Donors and Barriers to Return Four Years after the Initial Donation

    OpenAIRE

    Weidmann, Christian; Müller-Steinhardt, Michael; Schneider, Sven; Weck, Eberhard; Klüter, Harald

    2011-01-01

    Background: The aim of the study was to identify characteristics of lapsed donors 4 years after the initial donation as well as self-reported barriers to return for further blood donations. Methods: A random number of 8,000 blood donors, donating for the German Red Cross Blood Service Baden-Wurttemberg - Hessen, were asked to fill in a self-administered questionnaire. The response rate was 38.5%. Donors were categorized as ”lapsed’ if they had not donated within the last 24 months. The odds o...

  2. Automatic system for blood donors control with seropositive results in epidemiological tests.

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    Ariel Menéndez Barrios

    2008-04-01

    Full Text Available Background: Prevention of infectious diseases transmission through blood transfusion is one of the biggest challenges in the transfusion field of medicine. Objective: to design a computerized system for blood donors control with seropositive results in epidemiological tests. Methods: A system designed by the Provincial Blood Bank using Microsoft Access as a data base system and DELFHI 7 as associated language. It runs under Windows 2000 or any other superior system with 64 RAM and do not need special requirements of software or hardware. It includes the following data: Full name, age, sex, race, Id number, home address, number of the clinical record in the blood bank, date when the trial took place in each patient, kind of disease diagnosed, technical data of the trial such as: fluorescence, sectional level of the 1st trial as well as the repetition, name of the technician who performed it. Results: It permits to consult general data of donors as well as the disease diagnosed. It also permits to elaborate reports of these patients from any PC of the blood bank acceding with the security password of the program. Conclusions: It constitutes a beneficial tool which permits to improve the notification system established in the program of the web about sexually transmitted diseases from the Minister of Public Health in Cuba.

  3. TRENDS OF TRANSFUSION TRANSMITTABLE INFECTIONS AMONG VOLUNTARY BLOOD DONORS IN A TERTIARY CARE HOSPITAL, MANDYA

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    Manjunath

    2014-09-01

    Full Text Available INTRODUCTION: Transmission of infectious diseases through donated blood is of concern to blood safety as transfusion forms an integral part of medical and surgical therapy. Blood transfusion carries the risk of transfusion-transmissible infections including HIV, hepatitis etc. Screening of voluntary donors who represent healthy population serves as a predictor for these dreadful diseases in healthy population. MATERIALS AND METHODS: This retrospective study was conducted at the blood bank of MIMS, Mandya. Data were analyzed for a period of 5 years from Jan 2009 to Dec 2013. All voluntary donors reporting to the blood bank were screened for hepatitis B (HBV, Hepatitis C Virus (HCV, HIV by using ELISA. The Rapid plasma regain test (RPR test was used for estimation of syphilis infection. RESULTS: The most common infection was hepatitis B (1.2% followed by HIV infection (0.27% and HCV (0.09% in our study. CONCLUSION: This study has shown a decrease in seroprevalence of HIV and increase in seroprevalence of HCV over the 5 year study period.

  4. Selective Immunoglobulin A Deficiency in Iranian Blood Donors: Prevalence, Laboratory and Clinical Findings

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    Saghafi Shiva

    2008-09-01

    Full Text Available Selective deficiency of immunoglobulin A (IgA is the most frequent primary hypogammaglobulinemia. As some IgA-deficient patients have IgA antibodies in their plasma which may cause anaphylactic reactions, blood centers usually maintain a list of IgA-deficient blood donors to prepare compatible blood components. In this study we determined the incidence of selective IgA deficiency (SIgAD in normal adult Iranian population. 13022 normal Iranian blood donors were included in this study. The assay which we used was adapted to the manual pipetting system and ELISA reader was used for screening. Other classes of immunoglobulins (G, M, as well as secretory IgA and IgG subclasses were tested in IgA deficient cases by ELISA. SPSS was used for statistical analysis.Among 13022 studied cases, 11608 blood donors were males (89.14% and 1414 were females (10.86%. Their mean (±SD age and weight were 38.5±11 years and 82±12 Kg respectively. Twenty of the screened samples were found by means of ELISA to be IgA-deficient (less than 5mg/dl, (frequency; 1:651. The data could indicate a compensation for IgA deficiency by serum IgM in one of our IgA deficient cases (Patient 5. We observed a correlation between IgG3 and serum IgA in deficient cases (r=0.498, P=0.025. Our results indicate that in present study the prevalence of S IgA D is in agreement with data from other Caucasians populations (from 1:300 to 1:700. In conclusion, Selective IgA Deficiency could be almost asymptomatic in most cases in general population. Our study suggests that; due to high frequency of IgA deficiency in Iran, it seems necessary to measure IgA levels for every blood donor and blood recipient to find IgA deficient cases.

  5. Motivations, experiences, and perspectives of bone marrow and peripheral blood stem cell donors: thematic synthesis of qualitative studies.

    Science.gov (United States)

    Garcia, Maria C; Chapman, Jeremy R; Shaw, Peter J; Gottlieb, David J; Ralph, Angelique; Craig, Jonathan C; Tong, Allison

    2013-07-01

    Hematopoietic stem cell (HSC) transplantation using bone marrow and peripheral blood stem cells is a lifesaving treatment for patients with leukemia or other blood disorders. However, donors face the risk of physical and psychosocial complications. We aimed to synthesize qualitative studies on the experiences and perspectives of HSC donors. We searched MEDLINE, Embase, PsycINFO, CINAHL, Google Scholar, and reference lists of relevant articles to November 13, 2012. Thematic synthesis was used to analyze the findings. Thirty studies involving 1552 donors were included. The decision to donate included themes of saving life, family loyalty, building a positive identity, religious conviction, fear of invasive procedures, and social pressure and obligation. Five themes about the donation experience were identified: mental preparedness (pervasive pain, intense disappointment over recipient death, exceeding expectations, and valuing positive recipient gains), burden of responsibility (striving to be a quality donor, unresolved guilt, and exacerbated grief), feeling neglected (medical dismissiveness and family inattention), strengthened relationships (stronger family ties, establishing blood bonds), and personal sense of achievement (satisfaction and pride, personal development, hero status, and social recognition). Although HSC donation was appreciated as an opportunity to save life, some donors felt anxious and unduly compelled to donate. HSC donors became emotionally invested and felt responsible for their recipient's outcomes and were profoundly grieved and disappointed if the transplantation was unsuccessful. To maximize donor satisfaction and mitigate the psychosocial risks for HSC donors, strategies to address the emotional challenges of anxiety, sense of coercion, guilt, and grief in donors are warranted. PMID:23603456

  6. Comparison of deferral rates using a computerized versus written blood donor questionnaire: a randomized, cross-over study [ISRCTN84429599

    Directory of Open Access Journals (Sweden)

    Bourque Ronald

    2002-08-01

    Full Text Available Abstract Background Self-administered computer-assisted blood donor screening strategies may elicit more accurate responses and improve the screening process. Methods Randomized crossover trial comparing responses to questions on a computerized hand-held tool (HealthQuiz, or HQ, to responses on the standard written instrument (Donor Health Assessment Questionnaire, or DHAQ. Randomly selected donors at 133 blood donation clinics in the area of Hamilton, Canada participated from 1995 to 1996. Donors were randomized to complete either the HQ or the DHAQ first, followed by the other instrument. In addition to responses of 'yes' and 'no' on both questionnaires, the HQ provided a response option of 'not sure'. The primary outcome was the number of additional donors deferred by the HQ. Results A total of 1239 donors participated. Seventy-one potential donors were deferred as a result of responses to the questionnaires; 56.3% (40/71 were deferred by the DHAQ, and an additional 43.7% (31/71 were deferred due to risks identified by the HQ but not by the DHAQ. Fourteen donors self-deferred; 11 indicated on the HQ that they should not donate blood on that day but did not use the confidential self-exclusion option on the DHAQ, and three used the self-exclusion option on the DHAQ but did not indicate that they should not donate blood on the HQ. The HQ identified a blood contact or risk factor for HIV/AIDS or sexually transmitted infection that was not identified by the DHAQ in 0.1% to 2.7% of donors. Conclusion A self-administered computerized questionnaire may increase risk reporting by blood donors.

  7. Phosphatidylserine exposure on stored red blood cells as a parameter for donor-dependent variation in product quality

    NARCIS (Netherlands)

    Dinkla, S.; Peppelman, M.; Raadt, J. van der; Atsma, F.; Novotny, V.M.J.; Kraaij, M.G.J. van; Joosten, I.; Bosman, G.J.C.G.M.

    2014-01-01

    BACKGROUND: Exposure of phosphatidylserine on the outside of red blood cells contributes to recognition and removal of old and damaged cells. The fraction of phosphatidylserine-exposing red blood cells varies between donors, and increases in red blood cell concentrates during storage. The susceptibi

  8. The use of vascular access ports for blood collection in feline blood donors

    OpenAIRE

    Aubert, Isabelle; Abrams-Ogg, Anthony C.G.; Sylvestre, Anne M.; Dyson, Doris H.; Allen, Dana G.; Johnstone, Ian B.

    2011-01-01

    We investigated vascular access ports for feline blood donation. Eight cats were anesthetized for conventional blood collection by jugular venipuncture at the beginning and end of the study. In-between conventional collections, vascular access ports were used for collection with or without sedation every 6 to 8 wk for 6 mo. Ports remained functional except for one catheter breakage, but intermittent occlusions occurred. Systolic blood pressure was lower during conventional collection. Behavio...

  9. Transfusion-Transmissible Infections among Voluntary Blood Donors at Wolaita Sodo University Teaching Referral Hospital, South Ethiopia.

    Science.gov (United States)

    Bisetegen, Fithamlak Solomon; Bekele, Fanuel Belayneh; Ageru, Temesgen Anjulo; Wada, Fiseha Wadilo

    2016-01-01

    Background. Transfusion-transmissible infections, human immunodeficiency virus, hepatitis B virus, hepatitis C virus, and syphilis are among the greatest threats to blood safety and pose a serious public health problem. Objective. To determine the magnitude of blood borne infections among blood donors at Wolaita Sodo University Teaching Referral Hospital. Methods and Materials. A cross-sectional study was conducted from 10/11/2015 up to 10/12/2015. 390 donors were consecutively included and data on donor's age, sex, blood type, and serum screening results were obtained by structured questionnaire and laboratory investigation. The collected data were entered into Epi Data version 1.4 and then exported to SPSS version 20.0 for analysis. Result. The seroprevalence of blood borne pathogens is 29.5% of which HCV, HBV, HIV, and syphilis account for 8.5%, 9.5%, 6.4%, and 7.5%, respectively. Multiple infections were observed among 2.8% of the infected individuals. In addition, age ≥ 30 has a significant association with HCV. Conclusion. Significantly higher prevalence of transfusion-transmissible infections was identified from blood donors and they remain to be the greatest threat to blood safety, so comprehensive screening of donors' blood for HIV, HBV, HCV, and syphilis using standard methods is highly recommended to ensure the safety of blood recipient. PMID:27597875

  10. Hepatitis B and C prevalences among blood donors in the south region of Brazil

    Directory of Open Access Journals (Sweden)

    H. C. F. F. Vasconcelos

    1994-12-01

    Full Text Available The prevalence of hepatitis B and C infection has been determined in a seroepidemiological survey among blood donors from the south of Brazil (Florianópolis, State of Santa Catarina. These markers has also been correlated with the levels of alanine aminotransferase (ALT, a surrogate marker to prevent post-transfusion hepatitis. Sera from 5000 donors were randomly collected in the period of April to November 1991. The prevalences of HBsAg, anti-HBs and anti-HBc were respectively 0.78, 7.02 and 13.98. The anti-HCV prevalence after confirmation testing with line immunoassay (LIA, was 1.14. Normal values of ALT ( = 70 U/ml in 2.48. The positivity of anti-HCV antibodies increased with the elevation of ALT levels. This correlation was not observed in relation to HBsAg. There exists a diversity in the recognition of HCV epitopes among HCV positive donors. Via the confirmation test used, we could observe that 94.7 of donors recognize the structural core antigen. Besides that, we observed that 5.26 of the HCV reactive sera recognized only epitopes located in the NS4 and/or NS5 region, indicating the importance of these epitopes for the improvement of assays.

  11. Low transfusion transmission of hepatitis E among 25,637 single-donation, nucleic acid-tested blood donors

    DEFF Research Database (Denmark)

    Harritshøj, Lene H.; Holm, Dorte K.; Sækmose, Susanne G.;

    2016-01-01

    investigated among Danish blood donors, and the prevalence of HEV transfusion-transmitted infection (TTI) was investigated among recipients. STUDY DESIGN AND METHODS: Samples from 25,637 consenting donors collected during 1 month in 2015 were screened retrospectively using an individual-donation HEV RNA...... immune response could not be excluded. Phylogenetic analysis showed relatively large sequence differences between HEV from donors, symptomatic patients, and swine. CONCLUSIONS: Despite an HEV RNA prevalence of 0.04% in Danish blood donations, all HEV-positive donations carried low viral loads, and no...... nucleic acid test with a 95% detection probability of 7.9 IU/mL. HEV-positive samples were quantified by real-time polymerase chain reaction and genotyped. Transmission was evaluated among recipients of HEV RNA-positive blood components. Phylogenetic analyses compared HEV sequences from blood donors...

  12. Seroepidemiology of Toxoplasma infection in blood donors in Jahrom District,Southern Iran

    Institute of Scientific and Technical Information of China (English)

    Mohammad; Hassan; Davami; Morteza; Pourahmad; Rasoul; Baharlou; Abdolreza; Sotoodeh; Jahromi; Abbass; Ahmadi; Vasmejani; Kavous; Solhjoo; Hamid; Reza; Fallah; Mohsen; Kalantari

    2015-01-01

    Objective:To identify the anti-Toxoplasma antibodies from blood donors who referred to blood transfusion bases of Jahrom County,using ELISA method.Methods:Based on the prevalence and characteristics method,400 serum samples were collected from blood donors referred to Jahrom blood transfusion bases,Southern Iran,during 2010–2011,designed at testing by ELISA.Ig M and Ig G antibodies against Toxoplasma gondii were tested using ELISA kits(Dia-Pro)on serums.The data were analysed by SPSS 19 software.Results:Review of 400 cases,54 of them were Ig G positive for parasites(13.5%)and 346of those with negative Ig G(86.5%).In Ig M examination,1.75%of them have been positive Ig M(7 cases)and 98.25%of them were Ig M negative(393 cases).By comparing the different group ages,40–50 year age group had the highest prevalence of Ig G positive(17.9%)and the age group of 30–40 years had the highest incidence of Ig M negative(2.5%).Conclusions:Due to the serological infection rate of toxoplasmosis obtained from this study,toxoplasmosis should be considered as a significant transfusion risk factor in Jahrom and also in any region with similar situations.

  13. Seroepidemiology of Toxoplasma infection in blood donors in Jahrom District, Southern Iran

    Institute of Scientific and Technical Information of China (English)

    Mohammad Hassan Davami; Morteza Pourahmad; Rasoul Baharlou; Abdolreza Sotoodeh Jahromi; Abbass Ahmadi Vasmejani; Kavous Solhjoo; Hamid Reza Fallah; Mohsen Kalantari

    2015-01-01

    Objective: To identify the anti-Toxoplasma antibodies from blood donors who referred to blood transfusion bases of Jahrom County, using ELISA method. Methods: Based on the prevalence and characteristics method, 400 serum samples were collected from blood donors referred to Jahrom blood transfusion bases, Southern Iran, during 2010–2011, designed at testing by ELISA. IgM and IgG antibodies against Toxoplasma gondii were tested using ELISA kits (Dia-Pro) on serums. The data were analysed by SPSS 19 software. Results: Review of 400 cases, 54 of them were IgG positive for parasites (13.5%) and 346 of those with negative IgG (86.5%). In IgM examination, 1.75%of them have been positive IgM (7 cases) and 98.25% of them were IgM negative (393 cases). By comparing the different group ages, 40–50 year age group had the highest prevalence of IgG positive (17.9%) and the age group of 30–40 years had the highest incidence of IgM negative (2.5%). Conclusions: Due to the serological infection rate of toxoplasmosis obtained from this study, toxoplasmosis should be considered as a significant transfusion risk factor in Jahrom and also in any region with similar situations.

  14. 献血者献血意愿及献血服务的Logistic回归研究%Donate willingness of blood donors and logistic regression to study blood donation service

    Institute of Scientific and Technical Information of China (English)

    赵燕

    2016-01-01

    ObjectiveExplore the donors of blood donation willingness and blood stations of zaozhuang services,to promote the sustainable development of unpaid blood donation.MethodsWith the method of questionnaire survey,from March to August in 2015,the basic situation of blood donors,blood donation,blood donors intend,source of information for blood donation knowledge,attitude to blood donation behavior,such as blood donation service for questionnaire survey. ResultsThis study as a sample,a total of 2 860 blood donors for the questionnaire survey,get 2 759 valid questionnaire responses received. Blood donors different gender,age,educational level,occupation,monthly income(yuan),the number of blood donation form than have significant difference(P<0.05)."Compassion" is the main blood donors will. Blood donation knowledge information sources are mainly "radio and television". Logistic regression analysis showed that sex,blood donation willingness,attitude,service is the main reason affecting the blood donors and respectively. ConclusionZaozhuang gender and blood donors blood donation willingness is the main reason for the effect of blood donation,blood stations staff service attitude is also the main reason for the effect of blood donation and blood environment.%目的:探讨献血者意愿和血站的献血服务,推动无偿献血工作的可持续发展。方法采用问卷调查的方式,于2015年3月—8月对来我站献血者的基本情况、献血经历、献血者意愿、获取献血知识的信息来源、献血服务等方面进行问卷调查。结果本研究共抽取2860献血者作为样本进行了问卷调查,得到2759份有效问卷。献血者不同性别、年龄、文化程度、职业、人月收入(元)、献血次数的构成比有显著性差异(P<0.05)。“奉献爱心”是献血者最主要的意愿。获取献血知识信息来源主要是“广播电视”。Logistic回归分析表明,性别、献血意愿、服务态度

  15. Skin Recurrence of Transformed Mycosis Fungoides Postumbilical Cord Blood Transplant despite Complete Donor Chimerism

    Directory of Open Access Journals (Sweden)

    Rahul Pawar

    2014-01-01

    Full Text Available Background. Allogeneic stem cell transplant is the treatment of choice for systemic cutaneous T-cell lymphoma (CTCL which provides graft-versus-lymphoma effect. Herein we discuss a case of recurrence of CTCL skin lesions after cord blood transplant in a patient who continued to have 100% donor chimerism in bone marrow. Case Presentation. A 48-year-old female with history of mycosis fungoides (MF presented with biopsy proven large cell transformation of MF. PET scan revealed multiple adenopathy in abdomen and chest suspicious for lymphoma and skin biopsy showed large cell transformation. She was treated with multiple cycles of chemotherapy. Posttherapy PET scan showed resolution of lymphadenopathy. Later she underwent ablative preparative regimen followed by single cord blood transplant. Bone marrow chimerism studies at day +60 after transplant showed 100% donor cells without presence of lymphoma. However 5 months after transplant she had recurrence of MF with the same genotype as prior skin lesion. Bone marrow chimerism study continued to show 100% donor cells. Conclusion. A differential graft-versus-lymphoma effect in our case prevented lymphoma recurrence systemically but failed to do so in skin. We hypothesize that this response may be due to presence of other factors in the bone marrow and lymph node microenvironments preventing recurrence in these sites.

  16. Recommendations for a standard UK approach to incorporating umbilical cord blood into clinical transplantation practice: an update on cord blood unit selection, donor selection algorithms and conditioning protocols.

    Science.gov (United States)

    Hough, Rachael; Danby, Robert; Russell, Nigel; Marks, David; Veys, Paul; Shaw, Bronwen; Wynn, Rob; Vora, Ajay; Mackinnon, Stephen; Peggs, Karl S; Crawley, Charles; Craddock, Charlie; Pagliuca, Antonio; Cook, Gordon; Snowden, John A; Clark, Andrew; Marsh, Judith; Querol, Sergio; Parkes, Guy; Braund, Henny; Rocha, Vanderson

    2016-02-01

    Allogeneic haemopoietic stem cell transplantation offers a potentially curative treatment option for a wide range of life-threatening malignant and non-malignant disorders of the bone marrow and immune system in patients of all ages. With rapidly emerging advances in the use of alternative donors, such as mismatched unrelated, cord blood and haploidentical donors, it is now possible to find a potential donor for almost all patients in whom an allograft is indicated. Therefore, for any specific patient, the transplant physician may be faced with a myriad of potential choices, including decisions concerning which donor to prioritize where there is more than one, the optimal selection of specific umbilical cord blood units and which conditioning and graft-versus-host disease prophylactic schedule to use. Donor choice may be further complicated by other important factors, such as urgency of transplant, the presence of alloantibodies, the disease status (homozygosity or heterozygosity) of sibling donors affected by inherited disorders and the cytomegalovirus serostatus of patient and donor. We report UK consensus guidelines on the selection of umbilical cord blood units, the hierarchy of donor selection and the preferred conditioning regimens for umbilical cord blood transplantation, with a summary of rationale supporting these recommendations. PMID:26577457

  17. Distribution of ABO and rhesus (D blood group antigens among blood donors at a tertiary care teaching hospital blood bank in south India

    Directory of Open Access Journals (Sweden)

    Suresh B

    2015-04-01

    Full Text Available Background: The ABO and Rhesus (Rh blood group systems are important for transfusion of blood and its components, organ transplantation, genetic studies and in medico-legal issues. Despite the long list of several other blood groups discovered so far, the knowledge and distribution of ABO and Rh-D blood group are essential for effective management of blood bank inventory. Methods: We retrospectively studied the distribution of ABO and Rh blood group antigens in donors presenting to our tertiary care teaching hospital blood bank in south India during the period January 2007 to August 2014. Blood group was determined by commercially available standard monoclonal antisera by test tube agglutination technique. Results: A total of 49,110 donor samples were tested during the study period for ABO grouping and Rh-D typing. Out of these 96.9% were males. The frequency of O, B, A, AB and Bombay blood groups were 41.7%, 32.2% 20%, 6.1% and 0.03% respectively. Rh (D positive and negative blood groups were seen in 92.8% and 7.2% respectively. The allele frequencies of the I A , IB and IO alleles were 0.1398, 0.2148 and 0.6454 respectively. In case of Rh-D group, the calculated gene frequencies for ID and Id were 0.7321 and 0.2679 respectively. Conclusion: Knowledge of blood group systems as documented in the present study helps in efficient management of blood bank and transfusion services in emergencies.

  18. Seroprevalence of Transfusion Transmissible Infections Among Voluntary Blood Donors at a Tertiary Care Teaching Hospital in Rural Area of India

    OpenAIRE

    Giri, Purushottam A; Jayant D Deshpande; Deepak B Phalke; Karle, Laximan B.

    2012-01-01

    Background: Blood transfusion is a life-saving measure in various medical and surgical emergencies. Transfusion medicine, apart from being important for the medical treatment of each patient, also has great public health importance. Objectives: The present study was conducted to estimate the prevalence of transfusion transmitted infections in voluntary blood donors at a rural tertiary care teaching hospital in western Maharashtra, India. Materials and Methods: All voluntary donors reporting t...

  19. Seroprevalence of Hepatitis B, Hepatitis C, and human immunodeficiency virus among healthy voluntary first-time blood donors in Kolkata

    OpenAIRE

    Das, B. K.; B K Gayen; Subhra Aditya; Sumit Kumar Chakrovorty; P.K. Datta; Ajay Joseph

    2011-01-01

    Background: Transfusion transmitted infections (TTIs) threaten safety of the recipients and the community as a whole and are a subject of real concern worldwide. Aims and Objectives: To know prevalence of Hepatitis-B (HBV), Hepatitis-C (HCV), and Human immunodeficiency virus (HIV) in voluntary first-time blood donors. Design and Setting: Cross-sectional observational study done in a teaching hospital. Materials and Methods: A total of 3 745 voluntary first-time blood donors were recruited and...

  20. Transfusion transmittable infections - Seroprevalence among blood donors in a tertiary care hospital of Delhi

    Directory of Open Access Journals (Sweden)

    Sangeeta Pathak

    2013-01-01

    Full Text Available Context: Transfusion transmittable infections (TTI continue to be a major threat to safe transfusion practices. Blood is one of the major sources of transmission of infectious diseases viz. human immunodeficiency virus (HIV, hepatitis B virus (HBV, hepatitis C virus (HCV, syphilis, malaria, and many other infections in India. Screening assays for the infectious diseases with excellent sensitivity and specificity helps to enhance the safety of the blood transfusions reducing the diagnostic window period as much as possible. Aims: The present study was designed to determine the seroprevalence of TTIs viz., HIV, HCV, and HBV, among the blood donors in Max Super Specialty Hospital, New Delhi, India based on dual testing strategy using high sensitive screening assays such as enhanced chemiluminescence assay and nucleic acid testing (NAT. Materials and Methods: A total of 41207 blood units collected from the donors (both voluntary and replacement donors were screened for the TTI s, viz., anti HIV 1 and 2 antibody, anti HCV antibody, anti HBcore antibody, and HBsAg by enhanced chemiluminescence assay on VITROS ® ECiQ immunodiagnostics system. NAT was performed using Roche Cobas ® TaqScreen MPX assay, which can detect simultaneously HIV 1 (groups M and O, HIV-2, HCV, and HBV on Roche Cobas ® s201 system. Results: The seroprevalence of HIV, HBsAg, anti HBcore antibody, and HCV based on enhanced chemiluminescence assay was found to be 0.25, 0.2, 7.06, and 0.7%, respectively. A total number of 6587 samples from July 2010 to December 2010 were tested on NAT, of which 3 samples were reactive for HBV in NAT; this was missed by enhanced chemiluminescence assay. Conclusions: Based on the seroprevalence study of infectious diseases viz., HIV, HBV, and HCV, we conclude that screening of blood and blood components by dual testing strategy using high sensitivity serological assay like enhanced chemiluminescence technology and NAT helps in detecting the

  1. Prospective evaluation of 2% (w/v alcoholic chlorhexidine gluconate as an antiseptic agent for blood donor arm preparation

    Directory of Open Access Journals (Sweden)

    Sweta Shah

    2014-01-01

    Full Text Available Aim: A prospective study was undertaken to evaluate the use of 2% (w/v alcoholic chlorhexidine gluconate (2% AlcCHG in donor arm preparation, to monitor the contamination rate of blood products after the collection and to find incidence of transfusion associated bacteremia. Settings and Design: Optimal skin antisepsis of the phlebotomy site is essential to minimize the risk of contamination. Food and Drug Administration (FDA in India has recommended antisepsis with three-step regimen of spirit-10% povidone iodine-spirit for donor arm antisepsis, but not with chlorhexidine, which is recommended by many other authors. Material and Methods: A total of 795 donors were studied from July 2011 to January 2012. Spirit-10% povidone iodine-spirit was used for 398 donors and 2% AlcCHG was used for 397 donors with the two-step method for arm antisepsis. Swabs were collected before and after use of antiseptic agents for all the donors. All the blood products collected from donors with growth in post-antisepsis swabs were cultured. A total of 123 various blood products were cultured irrespective of the method and result of antisepsis was observed. A total of seven patients had mild transfusion reaction. The transfused blood products, blood and urine specimen of the patients who had transfusion reaction were also cultured. Results: Seven donors out of 398 donors had growth in post-antisepsis swab with spirit-10% povidone iodine-spirit protocol and three donors out of 397 donors had growth in post-antisepsis swab with 2% AlcCHG protocol. All blood products collected from donors who had growth in post-antisepsis swabs when cultured had no growth. There was no contamination of blood products. Conclusions: Two percent (w/v alcoholic chlorhexidine gluconate with two-step protocol can be used as an antiseptic agent for donor arm preparation without considerable cost difference. It is at par with spirit 10% povidone iodine spirit protocol as suggested by FDA in India

  2. Factors associated with failure of clinical screening among blood donors who have altered serological results in the Centro Regional de Hemoterapia de Ribeirão Preto

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    Oranice Ferreira

    2012-01-01

    Full Text Available OBJECTIVE: This study aimed to investigate the frequency of positive results for hepatitis B and C, HIV and syphilis in blood donations at the Centro Regional de Hemoterapia de Ribeirão Preto, to describe donors with positive results according to some demographic and socioeconomic variables, to identify risk factors associated to these donors and the reasons that they were not detected during clinical screening. METHODS: A descriptive study was performed between July 1st 2005 and July 31st 2006 by interviewing 106 donors after medical consultations where they were informed of positive results for hepatitis B, hepatitis C, HIV or syphilis. RESULTS: There was a predominance of first-time donors, males, under 50-year olds, married individuals, from Ribeirão Preto, with elementary education, low economic status and of people who donated at the request of friends or relatives. Hepatitis C was the most frequently detected infection (56.6%, followed by hepatitis B (20.7%, HIV (12.3% and syphilis(10.4%. About 40% of donors had omitted risk factors for different reasons: because they trusted the results of serological tests, did not feel comfortable about talking of risk factors or did not consider them relevant. Other justifications were the duration of the interview, the interviewer was unskilled, embarrassment and doubts about confidentiality. CONCLUSION: The results indicate the need for changes in the approach to clinical screening and a review of methods to attract and guide potential donors.

  3. [Single-donor (apheresis) platelets and pooled whole-blood-derived platelets--significance and assessment of both blood products].

    Science.gov (United States)

    Hitzler, Walter E

    2014-01-01

    The transfusion efficacy of ATK, which contain fully functional platelets, is beyond all doubt. The equivalence of ATK and PTK has been subject of many studies. Some of those studies show the superiority of ATK's, while others do not, but there have been no studies that demonstrated a superiority of PTK's. The superiority of platelets stored in plasma and in third generation additive solution was demonstrated in clinical studies; therefore, it cannot be said that all the platelet concentrates on the German market are equivalent in efficacy. Of decisive importance, above all, is the risk of transfusion-transmitted infections with known pathogens, or those not yet discovered. This risk is different for ATK compared to PTK. Taking this difference in risk and the difference in donor exposure of transfused patients into account, it can definitely be said that ATK and PTK are not equivalent. In 2012, the Robert-Koch-Institute (RKI) published a mathematical risk model for different platelet concentrates and assessed the risk of transmitting known pathogens such as HIV, HCV, and HBV. The risk was higher for PTK compared to ATK. The relative risks for PTK derived from 4BCs were 2.2 (95%--CI: 2.1-2.4) for HIV, 2.7 (95%--CI: 2.5-3.0) for HCV, and 2.2 (95%--CI: 2.8-3.7) for HBV. At the present time, these are the relative risks of transfusion-transmitted infections with the traditional pathogens for PTK compared to ATK. In addition to the RKI assessed risks, there is the theoretical risk of a new, unknown agent, transmitted through blood exposure. The magnitude of this risk is hardly predictable for PTK. The experience gathered so far, especially in the last three decades, with the emergence of HIV, prions, and West Nil virus, shows that the biological nature of a next transfusion-transmissible infectious agent cannot be predictable. This agent, if we think at a conventional sexually transmissible agent with nucleic acid and long latent period, would spread first in areas with

  4. [Single-donor (apheresis) platelets and pooled whole-blood-derived platelets--significance and assessment of both blood products].

    Science.gov (United States)

    Hitzler, Walter E

    2014-01-01

    The transfusion efficacy of ATK, which contain fully functional platelets, is beyond all doubt. The equivalence of ATK and PTK has been subject of many studies. Some of those studies show the superiority of ATK's, while others do not, but there have been no studies that demonstrated a superiority of PTK's. The superiority of platelets stored in plasma and in third generation additive solution was demonstrated in clinical studies; therefore, it cannot be said that all the platelet concentrates on the German market are equivalent in efficacy. Of decisive importance, above all, is the risk of transfusion-transmitted infections with known pathogens, or those not yet discovered. This risk is different for ATK compared to PTK. Taking this difference in risk and the difference in donor exposure of transfused patients into account, it can definitely be said that ATK and PTK are not equivalent. In 2012, the Robert-Koch-Institute (RKI) published a mathematical risk model for different platelet concentrates and assessed the risk of transmitting known pathogens such as HIV, HCV, and HBV. The risk was higher for PTK compared to ATK. The relative risks for PTK derived from 4BCs were 2.2 (95%--CI: 2.1-2.4) for HIV, 2.7 (95%--CI: 2.5-3.0) for HCV, and 2.2 (95%--CI: 2.8-3.7) for HBV. At the present time, these are the relative risks of transfusion-transmitted infections with the traditional pathogens for PTK compared to ATK. In addition to the RKI assessed risks, there is the theoretical risk of a new, unknown agent, transmitted through blood exposure. The magnitude of this risk is hardly predictable for PTK. The experience gathered so far, especially in the last three decades, with the emergence of HIV, prions, and West Nil virus, shows that the biological nature of a next transfusion-transmissible infectious agent cannot be predictable. This agent, if we think at a conventional sexually transmissible agent with nucleic acid and long latent period, would spread first in areas with

  5. Phenotype frequencies of blood group systems (Rh, Kell, Kidd, Duffy, MNS, P, Lewis, and Lutheran in blood donors of south Gujarat, India

    Directory of Open Access Journals (Sweden)

    Manoj A Kahar

    2014-01-01

    Full Text Available Background: This is the first study on phenotype frequencies of various blood group systems in blood donors of south Gujarat, India using conventional tube technique. Material and Methods: A total of 115 "O" blood group donors from three different blood banks of south Gujarat were typed for D, C, c, E, e, K, Jk a , Le a , Le b , P 1 , M, and N antigens using monoclonal antisera and k, Kp a , Kp b , Fy a ,Fy b , Jk b , S,s, Lu a , and Lu b antigens were typed using polyclonal antisera employing Indirect Antiglobulin Test. Antigens and phenotype frequencies were expressed as percentages. Results: From the 115 blood donor samples used for extended antigen typing in the Rh system, e antigen was found in 100% donors, followed by D [84.35%], C [81.74%], c [56.32%], and E [21.74%] with DCe/DCe (R 1 R 1 , 40.87% as the most common phenotype. k was found to be positive in 100% of donors and no K+k- phenotype was found in Kell system. For Kidd and Duffy blood group system, Jk(a+b+ and Fy(a-b- were the most common phenotypes with frequency of 52.17% and 48.69%, respectively. In the MNS system, 39.13% donors were typed as M+N+, 37.39% as M+N-, and 23.48% as M-N+. S+s+ was found in 24.35% of donors, S+s- in 8.69%, and S-s+ as the commonest amongst donors with 66.96%. No Lu(a+b+ or Lu(a+b- phenotypes were detected in 115 donors typed for Lutheran antigens. A rare Lu(a-b- phenotype was found in 2.61% donors. Conclusion: Data base for antigen frequency of various blood group systems in local donors help provide antigen negative compatible blood units to patients with multiple antibodies in order to formulate in-house red cells for antibody detection and identification and for preparing donor registry for rare blood groups.

  6. ABO/Rh Blood Groups and Risk of HIV Infection and Hepatitis B Among Blood Donors of Abidjan, Côte D'ivoire.

    Science.gov (United States)

    Siransy, Liliane Kouabla; Nanga, Zizendorf Yves; Zaba, Flore Sandrine; Tufa, Nyasenu Yawo; Dasse, Sery Romuald

    2015-09-01

    Hepatitis B and HIV infection are two viral infections that represent real global public health problems. In order to improve their management, some hypotheses suggest that genetic predispositions like ABO and Rh blood groups would influence the occurrence of these diseases. The aim of the present study was to examine the association between ABO and Rhesus blood groups and the susceptibility to HIV infection and hepatitis B. We conducted a cross-sectional and analytical study in a population of voluntary blood donors in the Blood Transfusion Center of Abidjan. All blood donors who donated blood between January and June 2014 were tested for HBs antigen and anti-HIV antibodies (ELISA tests) and were ABO typed. The total number of examined blood donors during this period was 45,538, of which 0.32% and 8.07% were respectively infected with HIV and hepatitis B virus. O-group donors were more infected than non-O donors. Our study is an outline concerning the search for a link between ABO and Rh blood groups and hepatitis B and HIV infection. Further studies should be conducted to confirm the interaction between these two infections and contribute to the search for new therapeutic approaches.

  7. 22941 Cases of Blood Donor Screening Model of ALT%献血者22941例 ALT 初筛模式探讨

    Institute of Scientific and Technical Information of China (English)

    贺许华

    2013-01-01

    objective (ALT) unqualified and gender, age, occupation, type, body mass index, blood donation (BMI) and the relationship between life style, to explore the causes of elevated alt donors, establishment of donor alt screening model for blood donation, and provide scientific reference for recruitment. Methods td-1401a dry plate rapid detector for alt screening blood donors. Results The results of ALT in blood donors screening unqualified in male than in female P < 0.01 (χ2=110.840);different age donors, with the growth of the age of unqualified rate increased significantly P < 0.01 (χ2=280.110); different occupation, different types of blood donors have significant difference P < 0.01 ( χ2= 651.070, 416.490) blood donors; weight exceed the standard is the primary cause of alt failed P < 0.01 (χ2=764.390); lifestyle has become one of the factors of ALT in blood donors. Conclusion to strengthen the propaganda and education of health knowledge of blood donation, blood donors and different scales for different blood donation activities, the establishment of alt screening model, carry out alt screening, from low risk population were recruited donors, can effectively reduce the alt failure rate, saving precious blood resources.%目的:了解无偿献血者谷丙转氨酶(alt)不合格与性别、年龄、职业、献血类型、体重指数(BMi)及生活方式等的关系,探讨献血者 alt 升高的原因,建立献血者 alt 初筛模式,为无偿献血招募工作提供科学的参考依据。方法应用 td-1401a 干片式快速检测仪对献血者 alt 进行初筛。结果献血者 alt 初筛不合格中,男性显著高于女性P <0.01(χ2=110.840);不同年龄献血者,随年龄的增长不合格率显著上升P <0.01(χ2=280.110);不同职业、不同类型献血者有显著差异P <0.01(χ2=651.070,416.490);体重超标献血者是 alt 不合格的首要原因,P <0.01(χ2=764.390);生

  8. Sumatriptan increases the proliferation of peripheral blood mononuclear cells from HIV-infected individuals and healthy blood donors in vitro

    DEFF Research Database (Denmark)

    Afzelius, P; Nielsen, Jens Ole

    2000-01-01

    HIV infection is characterized by the loss of CD4+ T cells as well as the loss of T-cell function, leading to severe immunodeficiency. The proliferative capacity of T cells measured in vitro as responses to antigens and mitogens is severely reduced during HIV infection. An increased level...... responsible for regulation of the intracellular levels of cAMP. In a preliminary study sumatriptan increased the proliferative responses of PBMC to a polyclonal activator in vitro in 9 of 10 HIV-seropositive individuals (p=0.007), and in 7 of 9 healthy blood donors (p=0.05). This was probably due...... of the intracellular second messenger adenosine 3',5'-cyclic monophosphate (cAMP) has been shown to cause impaired proliferative capacity of peripheral blood mononuclear cells (PBMC) from HIV-infected individuals in vitro. Sumatriptan, a 5HT1d receptor agonist, inhibits the activity of adenylyl cyclases, the enzymes...

  9. Immunity to diphtheria and tetanus among blood donors in Arak, central province of Iran.

    Directory of Open Access Journals (Sweden)

    Ali Eslamifar

    2014-06-01

    Full Text Available Tetanus and diphtheria are vaccine-preventable, infectious diseases with significant morbidity and mortality. Immunization by the diphtheria and tetanus toxoid (DT has been applied in Iran for almost 50 years. However, there are very few data about the rate of immunity to these diseases in the adult population. the humoral immunity to tetanus and diphtheria among blood donors in Arak city, central provice of Iran were investigated.A total of 530 consecutive blood donor samples were collected from Blood Transfusion Organization, Central province of Iran. All samples were tested for diphteria and tetanus IgG antibodies using enzyme-linked immunosorbent assay (ELISA.From 530 cases, 91.9% were male and 8.1% were female. 99.6% of cases had protective levels of diphtheria antibody. Protective levels of tetanus antibody were found in 96% of subjects. There was not any significant difference between diphtheria and tetanus antibodies levels and age and sex.The obtained data showed that high proportion of the adult population in Arak have sufficient protection against diphtheria and tetanus. The high protective level of immunity to diphtheria and tetanus in Iran can be due to widespread use of booster vaccines in Iranian high schools and during the military services or for pregnant women in their 3(rd trimester.

  10. Phenotype frequencies of blood group systems (Rh, Kell, Kidd, Duffy, MNS, P, Lewis, and Lutheran) in blood donors of south Gujarat, India

    OpenAIRE

    Manoj A Kahar; Patel, Rajnikant. D.

    2014-01-01

    Background: This is the first study on phenotype frequencies of various blood group systems in blood donors of south Gujarat, India using conventional tube technique. Material and Methods: A total of 115 "O" blood group donors from three different blood banks of south Gujarat were typed for D, C, c, E, e, K, Jk a , Le a , Le b , P 1 , M, and N antigens using monoclonal antisera and k, Kp a , Kp b , Fy a ,Fy b , Jk b , S,s, Lu a , and Lu b antigens were typed using polyclonal antisera employin...

  11. Sibling cord blood donor program for hematopoietic cell transplantation: the 20-year experience in the Rome Cord Blood Bank.

    Science.gov (United States)

    Screnci, Maria; Murgi, Emilia; Valle, Veronica; Tamburini, Anna; Pellegrini, Maria Grazia; Strano, Sabrina; Corona, Francesca; Ambrogi, Eleonora Barbacci; Girelli, Gabriella

    2016-03-01

    Umbilical cord blood (UCB) represents a source of hematopoietic stem cells for patients lacking a suitably matched and readily available related or unrelated stem cell donor. As UCB transplantation from compatible sibling provides good results in children therefore directed sibling UCB collection and banking is indicated in family who already have a child with a disease potentially treatable with an allogeneic hematopoietic stem cell transplantation. Particularly, related UCB collection is recommended when the patients urgently need a transplantation. To provide access to all patients in need, we developed a "Sibling cord blood donor program for hematopoietic cell transplantation". Here we report results of this project started 20years ago. To date, in this study a total of 194 families were enrolled, a total of 204 UCB samples were successfully collected and 15 pediatric patients have been transplanted. Recently, some authors have suggested novel role for UCB other than in the transplantation setting. Therefore, future studies in the immunotherapy and regenerative medicine areas could expand indication for sibling directed UCB collection.

  12. West Nile Virus (WNV seroprevalence in a blood donors group of Milan

    Directory of Open Access Journals (Sweden)

    Giovanna Lunghi

    2012-09-01

    Full Text Available A seroprevalence study for anti West Nile virus was carried out among 864 healthy blood donors living in the metropolitan area of Milan by using a commercially available ELISA method. In addition, the performance of a novel ELISA assay for WNV antibodies was assessed. The sero-prevalence rate of WNV antibodies was 0.57% thus showing that WNV is likely circulating also in this up to now unknown area. The overall sensitivity and specificity of the novel ELISA were 99.9% and 45.4%, respectively, well comparable with that of the chosen reference immunoenzimatic method.

  13. Changing trends of seropositivity of HBsAg and HCV in blood donors

    OpenAIRE

    Agravat, Amit; Dobaria, Urvi; Dhruva, Gauravi

    2015-01-01

    Introduction: The term “Viral hepatitis” is reserved for infection of liver caused by a group of viruses having a particular affinity for liver. Liver disease due to Hepatitis B Virus is a huge problem globally with an estimated worldwide carrier rate of 25% of the total infected cases; every year there are over 4 million acute clinical cases of HBV infection.Method: All blood units donated by the voluntary donors are tested by serological method for the presence of various antigens or antibo...

  14. OCCULT HEPATITIS B VIRUS INFECTION AMONG BLOOD DONORS WITH ANTIBODIES TO HEPATITIS B CORE ANTIGEN

    OpenAIRE

    A Jafarzadeh; Kazemi Arababadi, M; M. Mirzaee A. Pourazar

    2008-01-01

    Diagnosis of hepatitis B is routinely based on of serological assay of hepatitis B surface antigen (HBsAg). Occult hepatitis B virus (HBV) infection is generally defined as the detection of HBV -DNA in the serum or tissues of subjects who have negative test for HBsAg. Transmission of HBV infection has been documented from HBsAg negative, anti-HBc positive blood and organ donors. The aim of this study was to determine the rate of occult HBV infection among HBsAg negative and anti-HBc positive ...

  15. Hepatitis G virus exposure in dialysis patients and blood donors in Isfahan-Iran

    OpenAIRE

    Maryam Salehi; Nader Kalbasi; Hassan Salehi; Nazila Kassaian; Marzieh Salehi; Farzin Khorvash; Mohamad Mehdi Salehi

    2014-01-01

    Background: Hepatitis G virus (HGV) is transmitted mainly by parenteral route and patients on maintenance hemodialysis (HD) are at risk for this infection. This study was conducted to estimate prevalence of infection through the presence of anti-HGV and to evaluate the clinical significance of HGV envelope protein E2 (anti-E2) in HD patients in compare with volunteer blood donors in Isfahan-Iran. Methods: In a cross-sectional study, a total of 40 HD patients as cases and 40 healthy volunt...

  16. [Significance of human leptospirosis in Mexico. Detection of Leptospira antibodies in a blood donor population].

    Science.gov (United States)

    Gavaldón, D G; Cisneros, M A; Rojas, N; Moles-Cervantes, L P

    1995-01-01

    The presence of specific serum antibodies has been used as a diagnostic test for human leptospirosis. The presence of these antibodies in humans is indicative of an active natural infection. Its detection after exposure denotes the presence of immunity. Serum samples from 206 adult blood donors were analyzed with a microscopic agglutination assay against 7 serovars of Leptospira interrogans. A total of 7% were positive with the following serovar distribution; shermani 53%, canicola 33%, pyrogens 20%, pomona 13% and icterohaemorrhagiae 6%. The highest frequency of seropositivity was found in the 20 year to 39 age group. These results in asymptomatic individuals show that leptospirosis is a frequent zoonosis in Mexico. PMID:8582567

  17. The results of nucleic acid testing in remunerated and non-remunerated blood donors in Lithuania

    Science.gov (United States)

    Kalibatas, Vytenis; Kalibatienė, Lina

    2014-01-01

    Background In Lithuania, governmentally covered remuneration for whole blood donations prevails. Donors may choose to accept or reject the remuneration. The purpose of this study was to compare the rate of nucleic acid testing (NAT) discriminatory-positive markers for human immunodeficiency virus-1 (HIV-1), hepatitis B virus (HBV) and hepatitis C virus (HCV) in seronegative, first-time and repeat, remunerated and non-remunerated donations at the National Blood Centre in Lithuania during the period from 2005 to 2010. Materials and methods All seronegative whole blood and blood component donations were individually analysed by NAT for HIV-1, HBV and HCV. Only discriminatory-positive NAT were classified. The prevalence of discriminatory-positive NAT per 100,000 donations in the donor groups and the odds ratios comparing the remunerated and non-remunerated donations were determined. Results Significant differences were observed for HBV NAT results: 47.42 and 26.29 per 100,000 remunerated first-time and repeat donations, respectively, compared to 10.6 and 3.58 per 100,000 non-remunerated first-time and repeat, seronegative donations, respectively. The differences were also significant for HCV NAT results: 47.42 and 51.99 for remunerated first-time and repeat donations, respectively, compared to 2.12 and 0 per 100,000 non-remunerated first-time and repeat, seronegative donations, respectively. No seronegative, discriminatory-positive NAT HIV case was found. The odds of discriminatory HBV and HCV NAT positive results were statistically significantly higher for both first-time and repeat remunerated donations compared to first-time and repeat non-remunerated donations. Discussion First-time and repeat remunerated seronegative donations were associated with a statistically significantly higher prevalence and odds for discriminatory-positive HBV and HCV NAT results compared to first-time and repeat non-remunerated donations at the National Blood Centre in Lithuania. PMID

  18. The development and specificity of antiidiotypic antibodies in renal transplant recipients receiving single-donor blood transfusions.

    Science.gov (United States)

    Phelan, D L; Rodey, G E; Anderson, C B

    1989-07-01

    Multiple pretransplant sera obtained from alloimmunized renal transplant recipients were tested for the presence of antiidiotypic-like antibodies (AB2) that inhibit donor-specific HLA antibodies in the microlymphocytotoxicity assay. Fourteen patients received repetitive single-donor blood transfusions (SDT). In this patient group, sera were collected prior to each blood transfusion and prior to transplantation. Three additional patients were studied in whom prior donor-specific HLA antibodies had been lost over a period of 6 months preceding transplantation. Donor-specific AB2-like antibodies were found in the sera of 13/14 SDT patients who did not develop HLA antibodies, and in the 3 patients who had lost donor-specific HLA antibodies. All patients had received prior random blood transfusions in the year preceding the study. Five (38%) of the SDT patients had detectable donor-specific AB2 prior to the initiation of single-donor blood transfusion, presumably related to previous blood transfusions. In the remaining six SDT patients in whom complete serum sets were available, AB2 always appeared after the first blood transfusion. The specificity of HLA antibodies inhibited by AB2 was studied, and antibodies against HLA-A, -B, -C, -DR, and DQw were all identified. Thus, there was no predilection for patients to develop AB2 against locus-specific HLA gene products. This study also confirms the apparent polymorphism of putative crossreactive idiotypes. Approximately 25% of donor-specific HLA antibodies were not inhibited by relevant AB2. This study confirms and extends previous observations that alloimmunization is associated in many patients with the development of antiidiotypic-like antibodies that are capable of inhibiting the binding and cytotoxicity of HLA alloantibodies. PMID:2473550

  19. Seroprevalencia de toxocarosis en donantes de sangre Toxocarosis seroprevalence in blood donors

    Directory of Open Access Journals (Sweden)

    N. Navarrete

    1998-01-01

    Full Text Available Se determinó, mediante la técnica de ELISA, la seroprevalencia de toxocarosis en 188 donantes de sangre del Hospital Base de Valdivia. El 74.5% de los donantes fueron del sexo masculino y el 25.5% del sexo femenino, con edad promedio de 29.9 ± 8.5 años. Se demostró la presencia de anticuerpos anti-Toxocara en el 5.3% de los donantes. El análisis estadístico no mostró diferencias significativas para la prevalencia de infección en relación a las variables de sexo, edad y procedencia del huésped.Toxocarosis seroprevalence in 188 blood donors of the County Hospital of Valdivia was determined using the ELISA test. 74.5% of the donors were male and 25.5% female, with an age average of 29.9 ± 8.5. Anti Toxocara antibodies were detected in 5.3% of the donors, and no significant differences were observed in relation to sex, age or origin

  20. [A large-scale survey for rare blood group screening among blood donors in Chinese over Nanjing area].

    Science.gov (United States)

    Ma, Ling; Liu, Yan-Chun; Xue, Min; Wei, Peng; Tang, Rong-Cai

    2011-02-01

    The purpose of this study was to investigate the distribution of 10 rare red blood groups in Chinese Nanjing population, so as to provide compatible rare blood to patients and to create a donor data bank. Jk (a-b-) (Kidd) phenotypes were detected by urea, while H-(H), GPA-(MNS), GPC-(Gerbich), i+ (Ii) and Lub-(Lutheran) phenotypes were detected by monoclonal, polyclonal antibodies with U type 96 well microplate technology. The screening of Jsb- and k-(Kell), Fya-(Duffy), Ok-(Ok), s-(MNS) and Dib-(Digeo) phenotypes were performed by polymerase chain reaction. The results showed that 2 Jk (a-b-) out of 40337 donation samples and 3 Fy (a-b+) out of 1782 donation samples were found, while no other rare blood phenotypes (H-, GPA-, GPC-, Lub-, Ok-, s-, Jsb-, k-, Dib- and i+) were detected. It is concluded that the frequencies of Jk (a-b-) and Fya(a-b+) are 0.0049% and 0.168% respectively. No more rare blood phenotype was found in this screening.

  1. Preoperative predictors of blood component transfusion in living donor liver transplantation

    Directory of Open Access Journals (Sweden)

    R N Makroo

    2013-01-01

    Full Text Available Context: Extensive bleeding associated with liver transplantation is a major challenge faced by transplant surgeons, worldwide. Aims: To evaluate the blood component consumption and determine preoperative factors that predict the same in living donor liver transplantation (LDLT. Settings and Design: This prospective study was performed for a 1 year period, from March 2010 to February 2011. Materials and Methods: Intra- and postoperative utilization of blood components in 152 patients undergoing LDLT was evaluated and preoperative patient parameters like age, gender, height, weight, disease etiology, hemoglobin (Hb, hematocrit (Hct, platelet count (Plt, total leukocyte count (TLC, activated partial thromboplastin time (aPTT, international normalized ratio (INR, serum bilirubin (T. bilirubin, total proteins (T. proteins, albumin to globulin ratio (A/G ratio, serum creatinine (S. creatinine, blood urea (B. urea, and serum electrolytes were assessed to determine their predictive values. Univariate and stepwise discriminant analysis identified those factors, which could predict the consumption of each blood component. Results: The average utilization of packed red cells (PRCs, cryoprecipitates (cryo, apheresis platelets, and fresh frozen plasma was 8.48 units, 2.19 units, 0.93 units, and 2,025 ml, respectively. Disease etiology and blood component consumption were significantly correlated. Separate prediction models which could predict consumption of each blood component in intra and postoperative phase of LDLT were derived from among the preoperative Hb, Hct, model for end-stage liver disease (MELD score, body surface area (BSA, Plt, T. proteins, S. creatinine, B. urea, INR, and serum sodium and chloride. Conclusions: Preoperative variables can effectively predict the blood component requirements during liver transplantation, thereby allowing blood transfusion services in being better prepared for surgical procedure.

  2. Dengue antibodies in blood donors Pesquisa de anticorpos antidengue em doadores de sangue

    Directory of Open Access Journals (Sweden)

    Rejane Cristina Ribas-Silva

    2012-01-01

    Full Text Available BACKGROUND: Dengue is an urban arbovirus whose etiologic agent is a virus of the genus Flavorius with four distinct antigen serotypes (DENV-1, DENV-2, DENV-3 and DENV-4 that is transmitted to humans through the bite of the mosquito Aedes aegypti. The Campo Mourão region in Brazil is endemic for dengue fever. OBTECTIVE: The aim of this study was to evaluate the presence of IgG and IgM antibodies specific to the four serotypes of dengue in donors of the blood donor service in the city of Campo Mourão. METHODS: Epidemiological records were evaluated and 4 mL of peripheral blood from 213 blood donors were collected in tubes without anticoagulant. Serum was then obtained and immunochromatographic tests were undertaken (Imuno-Rápido Dengue IgM/IgG TM. Individuals involved in the study answered a social and epidemiological questionnaire on data which included age, gender and diagnosis of dengue. RESULTS: Only three (1.4% of the 213 blood tests were positive for IgG anti-dengue antibodies. No donors with IgM antibody, which identifies acute infection, were identified. CONCLUSIONS: The results of the current analysis show that the introduction of quantitative or molecular serological methods to determine the presence of anti-dengue antibodies or the detection of the dengue virus in blood donors in endemic regions should be established so that the quality of blood transfusions is guaranteed.OBJETIVO: O objetivo desta pesquisa foi realizar uma triagem sorológica para detectar a presença de anticorpos IgG e IgM específicos para os quatro sorotipos da dengue em doadores de sangue atendidos pelo Hemonúcleo de Campo Mourão-PR. MÉTODOS: foram aplicadas fichas epidemiológicas e coletados 4 mL de sangue periférico em tubos sem anticoagulante de 213 doadores de sangue. Posteriormente, foi obtido o soro e realizado o teste imunocromatrográfico (Imuno - Rápido Dengue IgM/IgG. Os indivíduos envolvidos neste estudo responderam um questionário s

  3. New strategies for blood donor screening for hepatitis B virus: nucleic acid testing versus immunoassay methods.

    Science.gov (United States)

    Kuhns, Mary C; Busch, Michael P

    2006-01-01

    Serologic testing for hepatitis B virus (HBV) surface antigen (HBsAg) and antibody to HBV core antigen (anti-HBc) has historically been the foundation of blood screening, while HBV nucleic acid testing (NAT) was recently developed to detect HBsAg-negative, anti-HBc-negative blood units donated during early acute infection. Comparison data on seroconversion panels using HBsAg assays of varying sensitivities and pooled- or single-sample NAT, along with viral load estimates corresponding to HBsAg assay detection limits, have provided information on the theoretical benefits of NAT relative to HBsAg. Model-derived estimates have generally been predictive of the yields of DNA-positive, HBsAg-negative window period blood units detected in a number of studies from Europe, Japan, and the US. Studies indicate that the added benefit of pooled-sample NAT is relatively small in areas of low endemicity, with greater yields in areas highly endemic for HBV. Single-sample NAT would offer more significant early window period closure and could prevent a moderate number of residual HBV transmissions not detected by HBsAg assays; however, no fully automated single-sample HBV NAT systems are currently available.Even single-sample HBV NAT may not substitute for anti-HBc screening, as indicated by studies of donors with isolated anti-HBc who have extremely low DNA levels undetectable by standard single-sample NAT and who have been associated with transfusion-transmitted HBV. Moreover, HBsAg testing may still be needed even in the setting of combined anti-HBc and NAT screening. HBsAg-positive units from donors in the chronic stage of infection may contain very low or intermittently detectable DNA levels that single-sample NAT would miss. Although such donors are usually anti-HBc reactive and would be interdicted by anti-HBc screening, some lack anti-HBc. Extensive parallel testing will be needed to determine whether single-sample NAT in combination with anti-HBc might be sufficient to

  4. Challenges of using HIV as a primary risk indicator: Need for integrated blood donor risk management model

    NARCIS (Netherlands)

    Mapako, T.; Parirewa, J.J.; Emmanuel, J.C.; Mvere, D.A.; Massundah, E.; Mavunganidze, G.; Marowa, L.M.; Postma, M.J.; Van Hulst, M.

    2015-01-01

    Background: The use of risk modelling in blood safety is increasing getting momentum. NBSZ initiated blood donor risk profiling based on donation frequency (r-coding) since 1994 and in 2006 a generic risk classification model was developed (include age and donation venue) which was mainly based on H

  5. Italian national survey of blood donors: external quality assessment (EQA) of syphilis testing.

    Science.gov (United States)

    Vulcano, Francesca; Milazzo, Luisa; Volpi, Sabrina; Battista, Mara Maria; Barca, Alessandra; Hassan, Hamisa Jane; Pimpinelli, Fulvia; Giampaolo, Adele

    2010-03-01

    The detection of syphilis among blood donors may reveal high-risk sexual behavior, which can go unreported at the time of donor selection and compromise the safety of the donated blood. In Italy, blood is collected, tested, and distributed by transfusion services (TSs), which also perform outpatient transfusions. Although the TSs must screen for syphilis by law, there are no indications of the specific type of method to be used, generating discrepancies in the results obtained by the different TSs. To determine the proficiency of the TSs in screening for syphilis, we performed an external quality assessment (EQA). The EQA was based on two shipments of serum panels; 133 and 118 of the 326 existing TSs participated in the first and second shipments, respectively. Each panel consisted of both positive and negative serum samples. The results confirmed that the use of a single nontreponemal test (the Venereal Disease Research Laboratory [VDRL] and the rapid plasma reagin [RPR] tests) is the least sensitive means of identifying samples that are positive for syphilis antibodies. We also found that the interpretation of the results of manual techniques, such as the RPR test, the VDRL test, the Treponema pallidum hemagglutination (TPHA) assay, and the T. pallidum particle agglutination (TPPA) assay, can vary greatly among different TSs and operators. Total Ig enzyme immunoassays (EIAs) are the most sensitive. However, the determination of syphilis on the basis of the results of a single test is not sufficient for an accurate screening; and all blood units should thus be assessed by two distinct treponemal tests, that is, a total Ig EIA and the TPHA or the TPPA assay. PMID:20042617

  6. Trends in Seroprevalence of Hepatitis B, Hepatitis C, HIV, and Syphilis Infections in Iranian Blood Donors from 2003 to 2005

    OpenAIRE

    Farahnaz Fallahian; Bashir Hajibeigi; Farshid Alaeddini; Hassan Abolghasemi; Mohsen Amini; Seyyed-Mohammad Miri; Hossein Khedmat; Seyed-Moayed Alavian

    2009-01-01

    Background and Aims: To determine changes (trends) in infection rates of hepatitis B surface antigen (HBsAg), hepatitis C virus (HCV), human immunodeficiency virus (HIV), and syphilis in Iranian blood donor population.Methods: Specimens of 1,004,889 volunteer blood donors in Tehran blood transfusion service from 2003 to 2005 were screened for HBsAg, anti-HCV, anti-HIV1/2, and VDRL (venereal disease research laboratory) reactivity in a cross-sectional survey. Reactive samples were verified usi...

  7. Dengue seroprevalence of healthy adults in Singapore: serosurvey among blood donors, 2009.

    Science.gov (United States)

    Low, Swee-Ling; Lam, Sally; Wong, Wing-Yan; Teo, Diana; Ng, Lee-Ching; Tan, Li-Kiang

    2015-07-01

    Routine national notifications of dengue cases typically do not reflect the true dengue situation due to large proportion of unreported cases. Serosurveys, when conducted periodically, could shed light on the true dengue infections in the population. To determine the magnitude of dengue infections of the adult population in Singapore following the outbreak in 2007, we performed a cross-sectional study on blood donor samples from December 2009 to February 2010. The residual blood of 3,995 donors (aged 16-60 years) was screened for the presence of dengue-specific immunoglobulin G (IgG) and IgM using enzyme-linked immunosorbent assay (ELISA) kits. The age-weighted IgG prevalence of residents was 50.8% (N = 3,627, 95% confidence interval [CI] = 49.4-52.3%). Dengue IgG prevalence increased with age, with the lowest in 16-20 years age group (16.1%) and the highest in 56-60 years age group (86.6%). Plaque reduction neutralization test (PRNT) on samples of young resident adults (aged 16-30 years) revealed lower prevalence of neutralizing antibodies to each serotype, ranging from 5.4% to 20.3% compared with the older age groups. The level of exposure to dengue among the young adults is relatively low despite the endemicity of the disease in Singapore. It partially explains the population's susceptibility to explosive outbreaks and the high incidence rate among young adults.

  8. Healthy ranges of serum alanine aminotransferase levels in Tranian blood donors

    Institute of Scientific and Technical Information of China (English)

    Mehdi Mohamadnejad; Akram Pourshams; Reza Malekzadeh; Ashraf Mohamadkhani; Afsaneh Rajabiani; Ali Ali Asgari; Seyed Meysam Alimohamadi; Hadi Razjooyan; Mamar-Abadi

    2003-01-01

    AIM:The healthy ranges for serum alanine aminotransferase (ALT) levels are less well studied. The aim of this study was to define the upper limit of normal (ULN) for serum ALT levels, and to assess factors associated with serum ALT activity in apparently healthy blood donors.METHODS: A total of 1 939 blood donors were included.ALT measurements were performed for all cases using the same laboratory method. Healthy ranges for ALT levels were computed from the population at the lowest risk for liver disease. Univariate and multivariate analyses were performed to evaluate associations between clinical factors and ALT levels.RESULTS: Serum ALT activity was independently associated with body mass index (BMI) and male gender, but not associated with age. Association of ALT with BMI was more prominent in males than in females. Upper limit of normal for non-overweight women (BMI of less than 25) was 34 U/L,and for non-overweight men was 40 U/L.CONCLUSION: Serum ALT is strongly associated with sex and BMI. The normal range of ALT should be defined for male and female separately.

  9. Prevalence and factors associated with hepatitis C virus infection among Myanmar blood donors.

    Science.gov (United States)

    Myo-Khin; San-San-Oo; Oo, Khin May; Shimono, Kunio; Koide, Norio; Okada, Shigeru

    2010-10-01

    We studied the prevalence of hepatitis C virus infection among blood donors from 3 hospitals of Central Myanmar and 7 hospitals of Lower Myanmar in the Yangon area, and analyzed the factors associated with the infection. The study period was from November, 2005 to June, 2007. A pre-tested questionnaire was used to obtain information on age, ethnic group, marital status, tattooing, body piercing, history of receiving transfusions, and liver diseases in self and in sexual partners. Data on seropositivity to hepatitis C, hepatitis B and human immunodeficiency virus infections were recorded. A total of 65,240 blood donors participated in the study. Their ages ranged from 18 years to 60 years (mean±SD=29.5±9.3). The male-to-female ratio was 6:1. The prevalence of the antibody to hepatitis C was found to be 0.95% with varying rates (0.34 to 2.03) among hospitals. Females had a slightly higher rate (1.06%) than males (0.93%) (p=0.237). Multivariate analyses revealed the following factors to be related to HCV infection:HIV infection, odds ratio (OR)=3.0 (p=0.003); history of liver disease, OR=8.9 (p=0.001);and age 30 years and above, OR=2.6 (p=0.001). We discuss the varying prevalences of HCV around the world.

  10. Chronic hepatitis E virus infection after living donor liver transplantation via blood transfusion: a case report.

    Science.gov (United States)

    Kurihara, Takeshi; Yoshizumi, Tomoharu; Itoh, Shinji; Harimoto, Norifumi; Harada, Noboru; Ikegami, Toru; Inagaki, Yuki; Oshiro, Yukio; Ohkohchi, Nobuhiro; Okamoto, Hiroaki; Maehara, Yoshihiko

    2016-12-01

    Although it occurs worldwide, hepatitis E virus (HEV) infection in developed countries is generally foodborne. HEV infection is subclinical in most individuals. Although fulminant liver failure may occur, progression to chronic hepatitis is rare. This study describes a 41-year-old man with liver cirrhosis caused by non-alcoholic steatohepatitis and hepatocellular carcinoma within the Milan criteria. His liver function was classified as Child-Pugh grade C. Living donor liver transplantation (LDLT) was performed, and he was discharged from the hospital on postoperative day (POD) 22. However, his alanine aminotransferase concentration began to increase on POD 60 and HEV infection was detected on POD 81. Retrospective assessments of stored blood samples showed that this patient became positive for HEV RNA on POD 3. The liver donor was negative for anti-HEV antibodies and HEV RNA. However, the platelet concentrate transfused into the liver recipient the day after LDLT was positive for HEV RNA. The patient remained positive for HEV infection for 10 months. Treatment with 800 mg/day ribavirin for 20 weeks reduced HEV RNA to an undetectable level. In conclusion, this report describes a patient infected with HEV through a blood transfusion after LDLT, who progressed to chronic hepatitis probably due to his immunosuppressed state and was treated well with ribavirin therapy. PMID:27059470

  11. Quantification of print, radio and television exposure among previous blood donors in Kenya: an opportunity for encouraging repeat donation in a resource-limited setting?

    Science.gov (United States)

    Basavaraju, S V; Mwangi, J; Kellogg, T A; Odawo, L; Marum, L H

    2010-10-01

    Blood services in sub-Saharan Africa experience blood shortages and low retention of voluntary, non-remunerated donors. To boost collections by encouraging repeat donations, the Kenya National Blood Transfusion Service is exploring the likelihood of reaching previous donors through targeted print, radio and television advertising. We analysed data from a national AIDS Indicator Survey to determine whether previous donors have significant exposure to media. Respondents reporting history of blood donation had significantly higher exposure to print, radio and television media than those without history of blood donation. Targeted media campaigns encouraging repeat donation are likely to reach previous donors even in resource-limited settings. PMID:20598106

  12. Prevalence and Viral Load of Human Parvovirus B19 (B19V) Among Blood Donors in South-East Brazil.

    Science.gov (United States)

    Slavov, Svetoslav Nanev; Otaguiri, Katia Kaori; Covas, Dimas Tadeu; Kashima, Simone

    2016-06-01

    The infection of human parvovirus B19 (B19V) is a common event in the general population, including volunteer blood donors. In some cases it can be asymptomatic and can remain persistent for a long period of time. The objective of this study was to examine the B19V DNA prevalence and viral load in first-time volunteer blood donors. Blood samples were collected from 91 primary blood donors at the Regional Blood Center of Ribeirão Preto, Southeast Brazil. Viral detection and quantitation was performed by an in-house TaqMan(®) real-time PCR with high sensitivity. B19V DNA was detected in one male blood donor (1.0 %) and was characterized by a very low viral load (537.36 copies/mL). Our studies demonstrate that B19V DNA at low titer may be present in apparently healthy individuals. Sensitive molecular diagnostic tools can be applied for the screening of fresh blood derived products in order to prevent transfusion-transmitted B19V infection. PMID:27408426

  13. Umbilical cord blood units for public storage donors screening for markers of infectious agents

    Directory of Open Access Journals (Sweden)

    A. B. Smolyaninov

    2012-01-01

    Full Text Available The screening of 3515 cord blood samples which had entered bank for public use for presence of markers of infectious agents was carried out. It was established that majority of cord blood units contain markers of cytomegalovirus and Toxoplasma (81% that is not a contraindication for storage and subsequent use of these samples. In 4.6% of cases umbilical cord blood units were subjected to disposal because of identification of viral hepatitis B and C, as well as Tr. pallidum markers, moreover, the largest share of the discarded units contained antibodies to HbscorAg – 71,3%. Inclusion of analysis on the presence of Anti-HBcor in the required laboratory screening of mothers-donors CB was proposed in order to reduce the percentage of discarded umbilical cord blood units for the public inventory.

  14. Seroprevalence of human immunodeficiency virus in north Indian blood donors using third and fourth generation Enzyme linked immunosorbent assay

    OpenAIRE

    Sheetal Malhotra; Neelam Marwaha; Karan Saluja

    2013-01-01

    Background : The percentage of HIV cases attributable to blood transfusion has decreased significantly in the last decade. The newer 4 th generation Enzyme linked immunosorbent assay (ELISA) has been shown to have increased sensitivity compared to 3 rd generation ELISA. Objectives : To estimate the seroprevalence of HIV among blood donors using 4 th generation ELISA assay and to compare it with the 3 rd generation ELISA. Materials and Methods : This prospective study involved 10,200 blood don...

  15. Co-infection rate of HIV, HBV and Syphilis among HCV seropositive identified blood donors in Kathmandu, Nepal

    OpenAIRE

    Shrestha, Ashish Chandra; Ghimire, Prakash; Tiwar, Bishnu Raj; Rajkarnikar, Manita

    2012-01-01

    Background: HIV, HBV, Syphilis and HCV share common modes of transmission. Objective: The study was aimed to determine the co-infection rate of HIV, HBV and Syphilis among HCV seropositive identified blood donors. Methods: The study was conducted on blood samples screened as HCV seropositive at Nepal Red Cross Society, Central Blood Transfusion Service, Kathmandu, Nepal. HCV seropositive samples were further tested for HIV, HBV and Syphilis. Results: Eight co-infections were observed in 139 H...

  16. Serum γ-glutamyltransferase, alanine aminotransferase, and aspartate aminotransferase activity in Iranian healthy blood donor men

    Institute of Scientific and Technical Information of China (English)

    Hossein Khedmat; Nasrin Zarei; Farahnaz Fallahian; Hassan Abolghasemi; Bashir Hajibeigi; Zohre Attarchi; Farshid Alaeddini; Mohammad Taghi Holisaz; Masoumeh Pourali; Shahin Sharifi

    2007-01-01

    AIM: To determine serum γ-glutamyltransferase (GGT), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activity, and to assess their correlation with demographic and clinical findings in healthy blood donors.METHODS: This cross-sectional study was performed in 934 male blood donors, aged 18 to 68 years, who consecutively attended Tehran blood transfusion service in 2006. All participants were seronegative for HBV or HCV infections, non alcohol users, and all underwent a standard interview and anthropometric tests. Clinical and biochemical parameters including AST, ALT, and GGT activities were determined. Patients taking drugs known to cause hepatic fat deposition were excluded. For AST, ALT, and GGT variables, we used 33.33 and 66.66 percentiles, so that each of them was divided into three tertiles.RESULTS: Mean AST, ALT, and GGT activities were 25.26 ± 12.58 U/L (normal range 5-35 U/L), 33.13 ± 22.98 (normal range 5-35 U/L), and 25.11 ± 18.32 (normal range 6-37 U/L), respectively. By univariate analyses, there were significant associations between increasing AST, ALT, or GGT tertiles and age, body weight, body mass index, and waist and hip circumferences (P < 0.05). By multiple linear regression analyses, ALT was found to be positively correlated with dyslipidemia (B = 6.988, P = 0.038), whereas ALT and AST were negatively correlated with age. AST, ALT, and GGT levels had positive correlation with family history of liver disease (B = 15.763, P < 0.001), (B = 32.345, P < 0.001), (B =24.415, P < 0.001), respectively.CONCLUSION: Although we did not determine the cutoffs of the upper normal limits for AST, ALT, and GGT levels, we would suggest screening asymptomatic patients with dyslipidemia and also subjects with a family history of liver disease.

  17. The histopathological features of asymptomatic hepatitis C virus-antibody positive blood donors.

    Science.gov (United States)

    McMahon, R F; Yates, A J; McLindon, J; Babbs, C; Love, E M; Warnes, T W

    1994-06-01

    Since the introduction of screening for hepatitis C virus (HCV) in donated blood, the risk of contracting posttransfusion hepatitis has been greatly reduced and the test has led to the recognition of asymptomatic blood donors positive for anti-HCV antibodies. Following confirmation of the HCV status with second generation RIBA testing followed by counselling, 55 patients had full investigations, including liver biopsy. These were classified by the traditional chronic hepatitis system and were graded according to the Knodell and Scheuer histological activity indices. Seven of the biopsies were normal (12%), apart from minor degrees of steatosis in two. Eleven cases (20%) were in the chronic lobular hepatitis category without portal inflammation, while 37 cases showed portal inflammation, including 20 (36%) cases where chronic persistent hepatitis was the predominant feature and 17 cases (31%) where there was chronic active hepatitis with piecemeal necrosis. Features which have previously been described in chronic HCV-associated hepatitis were noted: portal lymphoid aggregates (58%), lymphoid follicles with germinal centres (15%), bile duct damage (11%), lobular inflammation (80%), sinusoidal mononuclear cell infiltration (26%), acidophil body formation (11%), and steatosis (47%). Fibrosis was present in 46% of cases but was generally of mild degree; 9% of biopsies demonstrated bridging fibrosis but no cases of cirrhosis were present. Even though serum transaminase levels correlated well with the presence of chronic hepatitis and with the Scheuer and Knodell activity indices, a proportion of patients with significant liver damage had normal transaminase levels, and this study suggests the need for liver biopsy in the evaluation of asymptomatic HCV-positive blood donors. PMID:7520412

  18. Molecular characterization, distribution, and dynamics of hepatitis C virus genotypes in blood donors in Colombia.

    Science.gov (United States)

    Mora, Mónica Viviana Alvarado; Romano, Camila Malta; Gomes-Gouvêa, Michele Soares; Gutiérrez, Maria Fernanda; Carrilho, Flair José; Pinho, João Renato Rebello

    2010-11-01

    Hepatitis C virus (HCV) is a frequent cause of acute and chronic hepatitis and a leading cause for cirrhosis of the liver and hepatocellular carcinoma. HCV is classified in six major genotypes and more than 70 subtypes. In Colombian blood banks, serum samples were tested for anti-HCV antibodies using a third-generation ELISA. The aim of this study was to characterize the viral sequences in plasma of 184 volunteer blood donors who attended the "Banco Nacional de Sangre de la Cruz Roja Colombiana," Bogotá, Colombia. Three different HCV genomic regions were amplified by nested PCR. The first of these was a segment of 180 bp of the 5'UTR region to confirm the previous diagnosis by ELISA. From those that were positive to the 5'UTR region, two further segments were amplified for genotyping and subtyping by phylogenetic analysis: a segment of 380 bp from the NS5B region; and a segment of 391 bp from the E1 region. The distribution of HCV subtypes was: 1b (82.8%), 1a (5.7%), 2a (5.7%), 2b (2.8%), and 3a (2.8%). By applying Bayesian Markov chain Monte Carlo simulation, it was estimated that HCV-1b was introduced into Bogotá around 1950. Also, this subtype spread at an exponential rate between about 1970 to about 1990, after which transmission of HCV was reduced by anti-HCV testing of this population. Among Colombian blood donors, HCV genotype 1b is the most frequent genotype, especially in large urban conglomerates such as Bogotá, as is the case in other South American countries. PMID:20872715

  19. Vivax malaria in a blood donor in Spain, relapse or a new infection in a malaria non-endemic country?

    Science.gov (United States)

    Rubio, J M; Jiménez Del Bianco, A I; Cervera-Alonso, Y; Fernandez-Garcia, M D; Lanza, M; Ta Tang, T H; Sevil Puras, F; Blanco, L

    2016-02-01

    Malaria is a vectorborne disease caused by protozoan of the genus Plasmodium, which can also be transmitted by the transfusion of infected red blood cells. One year after return from a travel to Honduras, a Spanish traveller developed vivax malaria. Prior to the onset of symptoms, the donor made a donation that tested non-reactive using an immunological test for malaria. Samples from the donor taken before donation and tested by serological and molecular methods were negative but positive at the time of hospital admission. The possible sources of the donors' infection, imported versus locally acquired, are discussed. PMID:26509738

  20. A study on patterns of co-infections among blood donors at the blood bank of a tertiary care referral teaching hospital in South India

    Directory of Open Access Journals (Sweden)

    Suresh B

    2016-04-01

    Full Text Available Background: Blood serves as a vehicle for transmission of blood-borne pathogens including human immunodeficiency virus (HIV, hepatitis B virus (HBV, hepatitis C virus (HCV, malaria parasite (MP and syphilis. Safe blood and blood products should be transfused to all patients in need for blood transfusion. Material and Methods: All blood donors attending to the blood bank during the period January 2009 to December 2014 were screened for hepatitis B surface antigen (HBsAg, anti HCV antibody, anti HIV-1, 2 antibodies and HIV p24 antigen by using the appropriate enzyme-linked immunosorbent assay (ELISA method and further confirmed using an ELISA kit from a different manufacturer. Malarial antigen testing was done by rapid diagnostic device, which is based on immunochromatographic technique. The rapid plasma reagin (RPR test was used for estimation of syphilis infection and further confirmed by Treponema pallidum haemagglutination assay (TPHA. Results: Of the 41,785 donors who were screened during the study period, 20 (0.05% were reactive for different combination of infections. The various combination of infections seen were as follows; HBV+HCV and HBV+HIV (6/20 each, HIV+HCV (3/20, HIV + syphilis (2/20 and HBV+HIV+HCV, HBV+MP, HBV+syphilis (1/20 each; and HIV+Syphilis constituted for 10% (2/20. Conclusion: A properly conducted donor screening, notification and counseling of permanently deferred donors will help in reducing these co-infection rates.

  1. Correlation of hepatitis C RNA and serum alanine aminotransferase in hepatitis B and C seronegative healthy blood donors

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    Ali Natasha

    2010-07-01

    Full Text Available Introduction: Historically, serum alanine transaminase (ALT has been used as a surrogate marker in the detection of hepatitis viruses in blood donors. With the availability of newer sensitive technologies for the detection of seroconversion, the value of ALT becomes questionable but continues to be used for this purpose with subsequent discarding of ALT elevated blood units. Objective: The present study aims to evaluate the significance and cost effectiveness of ALT as a surrogate marker for hepatitis C virus infection in healthy asymptomatic blood donors who were serologically negative. Materials and Methods: The study was conducted at clinical laboratory of a tertiary care hospital for a period of one year from November 2006 to October 2007. All donors were screened serologically for hepatitis B, C and HIV I and II, syphilis and malaria and those tested positive were excluded from further evaluation. Gender-wise reference ranges and minimal and markedly raised results for ALT (described respectively as one and two folds increase above reference range were defined and, accordingly, donors were grouped into three. Two hundred seronegative blood donors were randomly selected from all three groups of ALT results and tested for hepatitis C nucleic acid through Amplicor; HCV RNA test. The cost of discarding an ALT -only elevated blood unit was also assessed. During the study period, 25117 subjects donated blood. Eight hundred and Results: seventy two donors (3.4% were positive for one or more serological tests. ALT of all donors ranged from 0-1501 U/L (Mean ± SD; 33.4 ± 25.45U/L. The donors seronegative for all disease markers were 24245 (96.6%. Of these, 21164 (87.2% donors had their ALT within reference range while 2874 (11.8% and 207 (0.8% of donors had minimal and markedly elevated results. Thus, 621 blood bags (red cells, platelets and plasma costing $ 39200.0 were discarded based on ALT results alone. Of 200 seronegative donors evaluated

  2. Seroprevalence of Hepatitis B, Hepatitis C, and human immunodeficiency virus among healthy voluntary first-time blood donors in Kolkata

    Directory of Open Access Journals (Sweden)

    B K Das

    2011-01-01

    Full Text Available Background: Transfusion transmitted infections (TTIs threaten safety of the recipients and the community as a whole and are a subject of real concern worldwide. Aims and Objectives: To know prevalence of Hepatitis-B (HBV, Hepatitis-C (HCV, and Human immunodeficiency virus (HIV in voluntary first-time blood donors. Design and Setting: Cross-sectional observational study done in a teaching hospital. Materials and Methods: A total of 3 745 voluntary first-time blood donors were recruited and tested for HBV, HCV, and HIV with Enzyme-linked immunosorbent assay (ELISA. Results and Analysis: Among 3 745 blood donors, majority (90.95% were male and 18 to 39 years age group. Prevalence of HBV was higher than HCV and HIV. HBV was maximum in 40 to 49 years (2.25% and 18 to 29 years (1.86% age group, whereas HCV and HIV were maximum in >50 years age group (0.93% and 1.86%, respectively. HIV positivity showed increasing prevalence with increase in age. Statistical Analysis: Statistical analysis was done using %, Chi square test, and Chi square for trend analysis. Conclusion: Seroprevalence of HBV, HCV, and HIV in voluntary blood donors is high in Kolkata and is high throughout this decade. Prevalence is even higher in other parts of India and Indian subcontinent. As voluntary blood donations are the major source of blood supply, chance of TTIs are very high in this part of the world.

  3. Prevalence of hepatitis B surface antigen & its subtypes in high risk group subjects & voluntary blood donors in Bombay.

    Science.gov (United States)

    Elavia, A J; Banker, D D

    1991-09-01

    HBsAg positive subjects belonging to high risk groups and voluntary blood donors were analysed for prevalence of HBsAg among various groups of subjects for ascertaining the carrier status among the voluntary blood donors, HBsAg subtype distribution, and association of HBsAg with blood groups and caste or religion. The prevalence of HBsAg varied from 2.02 per cent in voluntary blood donors to 58.38 per cent in patients of acute viral hepatitis. 70.5 per cent subjects had subtype 'ay' while 23.9 per cent of the subjects had subtype 'ad'. We also found compound 'ady' subtype in 5.6 per cent of our subjects. HBsAg/adr, a subtype not usually prevalent in India, was found in 30 of the 90 'ad' sera. Co-occurrence of HBsAg and anti-HBs was noted in 9 subjects. Homotypic anti-HBs was found to occur together mainly in voluntary blood donors, while heterotypic anti-HBs was found to occur together mainly multi-transfused patients. There was no significant correlation between HBsAg and blood group antigens and a relatively higher incidence of HBsAg among the Jain community was observed.

  4. Successful second transplantation from haploidentical donor for graft failure following unrelated cord blood cell transplantation or mismatched related transplantation: 2cases report

    Institute of Scientific and Technical Information of China (English)

    XU Lan-ping; HUANG Xiao-jun

    2006-01-01

    @@ Cord blood transplantation (CBT) from unrelated donors has increasingly been performed worldwide during the last decade. The immaturity of lymphocytes in cord blood permits HLA-mismatching between donors and recipients and reduces the severity of graft-versus-host disease (GVHD).However, the relatively small dose of the cord blood nucleated cells is associated with a high frequency of engraftment failure.1-5 But re-transplantation with stem cells from the original donor is impossible.

  5. Human T-Cell Lymphotropic Virus Types 1 and 2 Seropositivity among Blood Donors at Mbarara Regional Blood Bank, South Western Uganda

    Directory of Open Access Journals (Sweden)

    Patience Uchenna Tweteise

    2016-01-01

    Full Text Available Background. The human T-cell lymphotropic virus types 1 and 2 (HTLV 1/2 are retroviruses associated with different pathologies. HTLV-1 causes adult T-cell leukemia/lymphoma (ATL and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP; HTLV-2 is not clearly associated with a known clinical disease. Both viruses may be transmitted by whole blood transfusion, from mother to child predominantly through breastfeeding, and by sexual contact. Presently, none of the regional blood banks in Uganda perform routine pretransfusion screening for HTLV. The aim of this study was to determine the prevalence of anti-human T-cell lymphotropic virus types 1/2 (HTLV-1/2 antibodies among blood donors at Mbarara Regional Blood Bank in South Western Uganda. A cross-sectional study was conducted between June 2014 and September 2014. Methodology. Consecutive blood samples of 368 blood donors were screened for anti-HTLV-1/2 antibodies using an enzyme linked immunosorbent assay (ELISA. Samples reactive on a first HTLV-1/2 ELISA were further retested in duplicate using the same ELISA. Of the three hundred and sixty-eight blood donors (229 (62.2% males and 139 (37.8% females, only two male donors aged 20 and 21 years were HTLV-1/2 seropositive, representing a prevalence of 0.54%. Conclusion. HTLV-1/2 prevalence is low among blood donors at Mbarara Regional Blood Bank. Studies among other categories of people at risk for HTLV 1/2 infection should be carried out.

  6. Human T-Cell Lymphotropic Virus Types 1 and 2 Seropositivity among Blood Donors at Mbarara Regional Blood Bank, South Western Uganda.

    Science.gov (United States)

    Uchenna Tweteise, Patience; Natukunda, Bernard; Bazira, Joel

    2016-01-01

    Background. The human T-cell lymphotropic virus types 1 and 2 (HTLV 1/2) are retroviruses associated with different pathologies. HTLV-1 causes adult T-cell leukemia/lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP); HTLV-2 is not clearly associated with a known clinical disease. Both viruses may be transmitted by whole blood transfusion, from mother to child predominantly through breastfeeding, and by sexual contact. Presently, none of the regional blood banks in Uganda perform routine pretransfusion screening for HTLV. The aim of this study was to determine the prevalence of anti-human T-cell lymphotropic virus types 1/2 (HTLV-1/2) antibodies among blood donors at Mbarara Regional Blood Bank in South Western Uganda. A cross-sectional study was conducted between June 2014 and September 2014. Methodology. Consecutive blood samples of 368 blood donors were screened for anti-HTLV-1/2 antibodies using an enzyme linked immunosorbent assay (ELISA). Samples reactive on a first HTLV-1/2 ELISA were further retested in duplicate using the same ELISA. Of the three hundred and sixty-eight blood donors (229 (62.2%) males and 139 (37.8%) females), only two male donors aged 20 and 21 years were HTLV-1/2 seropositive, representing a prevalence of 0.54%. Conclusion. HTLV-1/2 prevalence is low among blood donors at Mbarara Regional Blood Bank. Studies among other categories of people at risk for HTLV 1/2 infection should be carried out.

  7. Burden of hepatitis C virus infection and its genotypes among the blood donors at Tirupati, Andhra Pradesh

    Directory of Open Access Journals (Sweden)

    Verma A

    2015-10-01

    Full Text Available Background: Safe blood donation remains a challenge in resource limited countries. False positive serological tests lead to wastage of large number of blood units. Nucleic acid amplification test (NAAT technology has greatly enhanced the accuracy in identification of transfusions transmitted infections. Methods: The present study was undertaken to study the seroprevalence of hepatitis C virus (HCV and asses the concordance between seropositivity with the presence of HCV ribonucleic acid (RNA and to know the distribution pattern of HCV genotypes in healthy blood donors. Results: Among the 9287 donors screened (88.3% males, 7153 (77% were voluntary donors while 2134 (23% were replacement donors. Among blood donors, 27 (0.3% samples tested HCV seropositive. Among 27 anti-HCV positive samples only 11 (41% were found positive for HCV RNA. Among the 5 samples subjected to sequencing, three were found to be genotype 1a while two were genotype 3a. Conclusions: Our observations suggest that implementing NAAT test for HCV screening will be helpful in minimizing false-positive test results in the Indian setting.

  8. An autoregressive integrated moving average model for short-term prediction of hepatitis C virus seropositivity among male volunteer blood donors in Karachi, Pakistan

    Institute of Scientific and Technical Information of China (English)

    Saeed Akhtar; Shafquat Rozi

    2009-01-01

    AIM: To identify the stochastic autoregressive integrated moving average (ARIMA) model for short term forecasting of hepatitis C virus (HCV) seropositivity among volunteer blood donors in Karachi, Pakistan. METHODS: Ninety-six months (1998-2005) data on volunteer blood donors tested at four major blood banks in Karachi, Pakistan were subjected to ARIMA modeling. Subsequently, a fitted ARIMA model was used to forecast HCV seropositive donors for 91-96 mo to contrast with observed series of the same months. To assess the forecast accuracy, the mean absolute error rate (%) between the observed and predicted HCV seroprevalence was calculated. Finally, a fitted ARIMA model was used for short-term forecasts beyond the observed series. RESULTS: The goodness-of-fit test of the optimum ARIMA (2,1,7) model showed non- s igni f icant autocorrelations in the residuals of the model. The forecasts by ARIMA for 91-96 mo closely followed the pattern of observed series for the same months, with mean monthly absolute forecast errors (%) over 6 mo of 6.5%. The short-term forecasts beyond the observed series adequately captured the pattern in the data and showed increasing tendency of HCV seropositivity with CONCLUSION: To curtail HCV spread, public health authorities need to educate communities and health care providers about HCV transmission routes based on known HCV epidemiology in Pakistan and its neighboring countries. Future research may focus on factors associated with hyperendemic levels of HCV infection.

  9. Evaluation of the baseline Widal titres in healthy blood donors of Uttarakhand

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    Garima Mittal

    2014-09-01

    Full Text Available Introduction: The interpretation of Widal test depends upon the baseline titre which is prevalent amongst healthy individuals in a particular geographical area. Aim and Objective: The aim of this study was to determine the baseline Widal titre of the apparently healthy population of a tertiary care centre of Uttarakhand. Material and Methods: Blood samples were collected from healthy blood donors (n=500 of the age group of 18-60 years, of both the sexes, who attended our blood bank from September 2013 to November 2013 and were analyzed for the presence of Salmonella antibodies by carrying out the Widal tube agglutination test. Results: Of the 500 serum samples which were tested, 255 (51% serum samples were positive for agglutinins (≥ 1:20 and 245 were negative. The most frequently recorded titre of the reactive sera was 1:40 for the anti-O antibodies and 1:80 for the anti-H antibodies for Salmonella enterica serotype typhi and this was the baseline titre. While the baseline titre of the ‘H’ agglutinins of Salmonella enterica serotype paratyphi A was 1:20 and of paratyphi B was also1:20. Conclusions: Based on the results of our study, it has been recommended that the significant titre of 1:80 for the anti-O antibodies and of 1:160 for the anti-H antibodies may be considered as diagnostic for enteric fever in the region of Uttarakhand, India.

  10. Behavior assessment of blood donors facing the risk of HIV infection, Abidjan, Cotê D'Ivoire, 2001-2002.

    Science.gov (United States)

    Minga, Albert K; Huët, Charlotte; Dohoun, Lambert; Abo, Yao; Bonard, Dominique; Gourvellec, G; Coulibaly, Ali; Konaté, Seidou; Dabis, Francois; Salamon, Roger

    2005-04-15

    Despite precautions taken to guarantee blood safety, in the National Blood Transfusion Center (CNTS) of Abidjan, about 30 regular blood donors are detected with HIV seroconversion each year, two-thirds of them men. A survey through face-to-face interviews was carried out at the CNTS of Abidjan from September 2001 to March 2002 among HIV-positive and HIV-negative regular blood donors, informed about their serologic status. HIV-negative regular blood donors informed about their serologic status since a median time of 67 months (n = 50) disclosed more risky behaviors such as multiple sexual partners (68%) than HIV-positive blood donors informed about their status (n = 112) since a median time of 35 months (41%) (P < 0.001). Condoms were systematically used by 17% of HIV-negative blood donors and 55% of HIV-positive blood donors (P < 0.001). Enhanced counseling and awareness could reduce in the future the number of cases of seroconversion among regular blood donors and improve their subsequent behavior. Blood donors who have unprotected sex with partners of unknown HIV serologic status and especially with casual partners are strongly exposed to HIV transmission and should be discouraged to continue giving blood, after adequate counseling.

  11. Various functions of PBMC from colon cancer patients are not decreased compared to healthy blood donors

    DEFF Research Database (Denmark)

    Afzelius, P; Nielsen, Hans Jørgen

    1997-01-01

    -2 and its receptor proteins in T helper cells. The proliferative responses and IL-2 synthesis of PBMC have earlier been shown to be reduced in patients with colon cancer. Recently immune modulating agents have been demonstrated to increase the proliferative response of PBMC in vitro, probably...... by inhibition of adenylate cyclase activity and induction of IL-2 mRNA expression. We have therefore studied the proliferative responses of PBMC from colon cancer patients to PWM and tested the effect of immune modulating agents, such as Serotonin, Sumatriptan, and Buspirone on these PBMC. We found...... no difference in levels of intracellular cAMP, IL-2 mRNA expression, IL-2R mRNA expression, or proliferative responses of PBMC from colon cancer patients compared to healthy blood donors. There was no effect of the immune modulating agents on PBMC from colon cancer patients....

  12. Plasma selenium levels in healthy blood bank donors in the central-eastern part of Belgium.

    Science.gov (United States)

    Van Cauwenbergh, Rudy; Robberecht, Harry; Van Vlaslaer, Veerle; De Smet, Annie; Emonds, Marie-Paule; Hermans, Nina

    2007-01-01

    Graphite furnace atomic absorption spectrometry, with Zeeman background correction and after improved matrix modification, was used to measure the plasma selenium content of healthy blood bank donors in the central part of Belgium. The mean plasma selenium concentration of 80 men and 80 women was 79.7+/-4.4ng/mL with a range of 55.0-117.4ng/mL. There was no gender difference observed. Plasma selenium level was significantly highest for the adult group, aged 45-64 years, compared to the others, except the young adults (18-24 years). The mean plasma selenium concentration measured corresponded well with literature data for Belgium. The obtained values were found to be in the medium range, compared with recent literature values for the European countries. PMID:17980813

  13. A study in the seroprevalence of hepatitis B core antibody and other transfusion transmitted infections in blood donors

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    R. Raj Bharath

    2016-06-01

    Results: Of the total 6954 donor blood samples which were tested, 713 samples (10.2% were found to be positive for anti-HBc (IgM and IgG and 93 samples (1.3% were found to be positive for HBsAg. Conclusions: Our study helped in determining the prevalence of hepatitis B core antibody and its subsequent detrimental effect on the blood donor population due to its high prevalence rate. [Int J Res Med Sci 2016; 4(6.000: 2337-2340

  14. Seroprevalence of hepatitis b in healthy blood donors at a teaching hospital of Kashmir (skims medical college and hospital Bemina, Srinagar): a restrospective study of five years

    OpenAIRE

    Mohd Younus Shah; Mohd Mubarik Naqash; Faizan Younus Shah

    2016-01-01

    Background: The study was conducted at the blood bank of the hospital. It was a retrospective analysis of blood donors over a period of five years from April 2008 to March 2013. Methods: 5ml blood sample was collected from the blood bag of each donor. 2ml blood was kept for testing hepatitis B. Bioelisa HBsAg color (a 3rd generation ELISA kit) was used for the screening of donor blood for Hepatitis B. All reactive samples were tested again using the same ELISA kit. Samples showing repeat t...

  15. Blood discard rate and the prevalence of infectious and contagious diseases in blood donors from provincial towns of the state of Parana, Brazil

    Directory of Open Access Journals (Sweden)

    Sueli Donizete Borelli

    2013-01-01

    Full Text Available Background: So that an improvement in the selection of donors can be achieved and the risk to the recipient of transfused blood can be reduced, prospective donors are submitted to clinical and serological screening. Objective: This study investigated the blood discard rate and the rate of infectious and contagious diseases in blood donors from provincial towns of the state of Paraná, Brazil. Methods: This study was an exploratory cross-sectional descriptive investigation with a quantitative approach of donations between January and December 2011. Results: In the study period the Regional Blood center in Maringá, Brazil received 8337 blood donations from people living in the city and neighboring towns. However, 278 (3.33% donations were discarded during serological screening owing to one or more positive serological markers. A total of 46.4% of the discarded blood units were confirmed positive by serology with anti-HBc being the most common (66.7%, followed by syphilis (22.5%, HBsAg (4.7%, anti-hepatitis C virus (3.1%, human immunodeficiency virus (1.5% and Chagas' disease (1.5%. The rate of infectious-contagious diseases that can be transmitted by blood transfusions was 1.55% (129/8337 of the donor population with a frequency of 1.03% for anti-HBc and 0.35% for syphilis. Conclusion: This study demonstrates a high prevalence of the anti-HBc marker in prospective blood donors from provincial towns in the state of Paraná, Brazil.

  16. Accuracy of a probabilistic record-linkage methodology used to track blood donors in the Mortality Information System database

    Science.gov (United States)

    Capuani, Ligia; Bierrenbach, Ana Luiza; Abreu, Fatima; Takecian, Pedro Losco; Ferreira, João Eduardo; Sabino, Ester Cerdeira

    2016-01-01

    The probabilistic record linkage (PRL) is based on a likelihood score that measures the degree of similarity of several matching variables. Screening test results for different diseases are available for the blood donor population. In this paper, we describe the accuracy of a PRL process used to track blood donors from the Fundação Pró-Sangue (FPS) in the Mortality Information System (SIM), in order that future studies might determine the blood donor’s cause of death. The databases used for linkage were SIM and the database made up of individuals that were living (200 blood donors in 2007) and dead (196 from the Hospital das Clinicas de São Paulo that died in 2001–2005). The method consists of cleaning and linking the databases using three blocking steps comparing the variables “Name/Mother’s Name/ Date of Birth” to determine a cut-off score. For a cut-off score of 7.06, the sensitivity and specificity of the method is 94.4% (95%CI: 90.0–97.0) and 100% (95%CI: 98.0–100.0), respectively. This method can be used in studies that aim to track blood donors from the FPS database in SIM. PMID:25210903

  17. Trends in Seroprevalence of Hepatitis B, Hepatitis C, HIV, and Syphilis Infections in Iranian Blood Donors from 2003 to 2005

    Directory of Open Access Journals (Sweden)

    Farahnaz Fallahian

    2009-02-01

    Full Text Available Background and Aims: To determine changes (trends in infection rates of hepatitis B surface antigen (HBsAg, hepatitis C virus (HCV, human immunodeficiency virus (HIV, and syphilis in Iranian blood donor population.Methods: Specimens of 1,004,889 volunteer blood donors in Tehran blood transfusion service from 2003 to 2005 were screened for HBsAg, anti-HCV, anti-HIV1/2, and VDRL (venereal disease research laboratory reactivity in a cross-sectional survey. Reactive samples were verified using a recognized confirmatory test which consisted of a second independent HBsAg enzyme immunoassay (EIA, and neutralization assay; an additional independent anti-HCV EIA and HCV-RIBA assay; a second independent anti-HIV1/2 test, and HIV Western blot; and a confirmatory fluorescent Treponemal antibody absorbed (FTA-ABS test, respectively. Results: The seroprevalence of HBsAg, anti-HCV, HIV Ab1/2, and VDRL was 0.9%, 2.1%, 0.2%, and 0.04%, respectively, in all blood donors. Prevalence of confirmed positivity was 0.6%, for HBsAg, 0.1%, for HCV RNA, 0.004%, for HIV western blot and 0.004% for FTA-ABS. Between 2003 to 2005, a decreasing trend was observed in HBsAg frequency. HCV frequency decreased in 2005 compared to 2003. The trend of HIV infection frequency had no increasing or decreasing pattern, and was relatively stable while the trend of syphilis infection frequency increased.Conclusions: Although the frequency of transfusion-transmitted infections is low, it is still far from ideal in the volunteer blood donors. Reduction of trends of infections could be achieved through more scrutiny in donor selection, improved sensitivity of serological tests, and re-evaluation of infection routes in donors.

  18. Scotblood 2015: Improving and delivering blood products, novel cellular therapies, and celebrating patients and donor engagement within transfusion services.

    Science.gov (United States)

    Colligan, David; McGowan, Neil; Seghatchian, Jerard

    2016-08-01

    Blood Transfusion Services are striving to continually improve the efficacy and quality of their blood products whilst also simultaneously diversifying into novel cellular products. For this to be successful the relationships between the various arms of the organisation must be strong and interlinked. As new technologies impact on the products that blood transfusion services supply it should be noted that the interaction between the service and its donor base is also affected by advancing technologies. Social media has fundamentally altered the way in which the public can access information and news, as such blood services must engage and interact appropriately with these new forms of media. As a reflection of these challenges the Scotblood 2015 programme was focussed on service and product improvement, donor engagement and people centred transfusion. This commentary comprises summaries of the presentations, based in part on the abstracts provided by the speakers. PMID:27524267

  19. Co-infection rate of HIV, HBV and Syphilis among HCV seropositive identified blood donors in Kathmandu, Nepal

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    Ashish Chandra Shrestha

    2012-02-01

    Full Text Available Background: HIV, HBV, Syphilis and HCV share common modes of transmission. Objective: The study was aimed to determine the co-infection rate of HIV, HBV and Syphilis among HCV seropositive identified blood donors. Methods: The study was conducted on blood samples screened as HCV seropositive at Nepal Red Cross Society, Central Blood Transfusion Service, Kathmandu, Nepal. HCV seropositive samples were further tested for HIV, HBV and Syphilis. Results: Eight co-infections were observed in 139 HCV seropositives with total co-infection rate of 5.75% (95% CI = 2.52-11.03. Conclusion: Co-infection of HIV, HBV and Syphilis with HCV is prevalent in the healthy looking blood donors of Kathmandu, Nepal.

  20. High prevalence of anti-hepatitis E virus antibodies among blood donors in central Italy, February to March 2014.

    Science.gov (United States)

    Lucarelli, Claudia; Spada, Enea; Taliani, Gloria; Chionne, Paola; Madonna, Elisabetta; Marcantonio, Cinzia; Pezzotti, Patrizio; Bruni, Roberto; La Rosa, Giuseppina; Pisani, Giulio; Dell'Orso, Luigi; Ragone, Katia; Tomei, Carla; Ciccaglione, Anna Rita

    2016-07-28

    Prevalence of anti-hepatitis E virus (HEV) antibodies is highly variable in developed countries, which seems partly due to differences in assay sensitivity. Using validated sensitive assays, we tested 313 blood donors attending a hospital transfusion unit in central Italy in January and February 2014 for anti-HEV IgG and IgM and HEV RNA. Data on HEV exposure were collected from all donors. Overall anti-HEV IgG prevalence was 49% (153/313). Eating raw dried pig-liver sausage was the only independent predictor of HEV infection (adjusted prevalence rate ratio = 2.14; 95% confidence interval: 1.23-3.74). Three donors were positive for either anti-HEV IgM (n = 2; 0.6%) or HEV RNA (n = 2; 0.6%); they were completely asymptomatic, without alanine aminotransferase (ALT) abnormalities. Of the two HEV RNA-positive donors (both harbouring genotype 3), one was anti-HEV IgG- and IgM-positive, the other was anti-HEV IgG- and IgM-negative. The third donor was positive for anti-HEV IgG and IgM but HEV RNA-negative. HEV infection is therefore hyperendemic among blood donors (80% men 18-64 years-old) from central Italy and associated with local dietary habits. Nearly 1% of donors have acute or recent infection, implying potential transmission to blood recipients. Neither ALT nor anti-HEV IgM testing seems useful to prevent transfusion-transmitted HEV infection. PMID:27494608

  1. Cost-Effectiveness of Blood Donor Screening for Babesia microti in Endemic Regions of the United States

    Science.gov (United States)

    Simon, Matthew S.; Leff, Jared A.; Pandya, Ankur; Cushing, Melissa; Shaz, Beth H.; Calfee, David P.; Schackman, Bruce R.; Mushlin, Alvin I.

    2014-01-01

    Background Babesia microti is the leading reported cause of red blood cell (RBC) transfusion-transmitted infection in the United States (US). Donor screening assays are in development. Study Design and Methods A decision analytic model estimated the cost-effectiveness of screening strategies for preventing transfusion-transmitted babesiosis (TTB) in a hypothetical cohort of transfusion recipients in Babesia-endemic areas of the US. Strategies included: (1) No screening, (2) Uniform Donor Health History Questionnaire (UDHQ), “status quo”, (3) Recipient risk-targeting using donor antibody (Ab) and polymerase chain reaction (PCR) screening, (4) Universal endemic donor Ab screening, (5) Universal endemic donor Ab and PCR screening. Outcome measures were TTB cases averted, costs, quality-adjusted life years (QALYs) and incremental cost-effectiveness ratios ($/QALY). We assumed a societal willingness to pay of $1 million/QALY based on screening for other transfusion-transmitted infections. Results Compared to no screening, the UDHQ avoids 0.02 TTB cases per 100,000 RBC transfusions at an incremental cost effectiveness ratio (ICER) of $160,000/QALY whereas recipient risk-targeted strategy using Ab/PCR avoids 1.62 TTB cases per 100,000 RBC transfusions at an ICER of $713,000/QALY compared to the UDHQ. Universal endemic Ab screening avoids 3.39 cases at an ICER of $760,000/QALY compared to the recipient-risk targeted strategy. Universal endemic Ab/PCR screening avoids 3.60 cases and has an ICER of $8.8 million/QALY compared to universal endemic Ab screening. Results are sensitive to blood donor Babesia prevalence, TTB transmission probability, screening test costs, risk and severity of TTB complications, and impact of babesiosis diagnosis on donor quality of life. Conclusion Antibody screening for Babesia in endemic regions is appropriate from an economic perspective based on the societal willingness to pay for preventing infectious threats to blood safety. PMID

  2. A case of nearly mistaken AB para-Bombay blood group donor transplanted to a group 'O' recipient.

    Science.gov (United States)

    Townamchai, Natavudh; Watanaboonyongcharoen, Phandee; Chancharoenthana, Wiwat; Avihingsanon, Yingyos

    2014-10-31

    Unintentional ABO mismatch kidney transplantation can cause detrimental hyperacute rejection. We report the first successful ABO incompatible kidney transplantation from an AB para-Bombay donor to O recipient. At the initial evaluation, the donor's ABO type was discordance on the cell typing and serum typing, which typed to be 'O' as cell typing and 'AB' as serum typing. At the second investigation, it was confirmed that the donor had a unique, rare but not uncommon blood type AB para-Bombay which was incompatible with the recipient's blood group. The kidney transplantation was successfully performed by an ABO incompatible preconditioning, double filtration plasmapheresis (DFPP) and rituximab. The serum creatinine at 12 months post-transplantation was 1.3 mg/dL. The pathology of the kidney biopsy showed no signs of rejection.

  3. [Epidemiologic study of anti-HTLV-I/II antibodies in blood donors in metropolitan France. The Retrovirus Working Group of the National Blood Transfusion Society].

    Science.gov (United States)

    Lemaire, J M; Coste, J; Barin, F; Couroucé, A M

    1991-01-01

    Between march and april 1989, the prevalence of HTLV-I/II antibodies was studied in a non selected population of 45,033 blood donors of Continental France. Serum samples were collected and screened by 12 Blood Banks located in different parts of the country. Screening was performed by 4 ELISAs (Abbott-Du Pont de Nemours-Organon-Pharmacia) and by gelatin particle agglutination (Fujirebio); the sensitivity of these tests was previously evaluated with an HTLV-I/II panel of the French National Society of Transfusion. The reproducibly reactive sera were confirmed by Western-Blot (Du Pont) and radioimmunoprecipitation assay. Five donors were confirmed positive: the global prevalence is 0.011%. This prevalence is one of the highest in Europe. Three of the five positive donors (0.007%) are white subjects. The other two are natives from the French West Indies (0.81%). Since may 1989 the Ministry of Health recommends for Continental France to test donors originating from endemic zones or to use their plasma for fractionation. The results obtained in this study show that the efficacy of these measures are incomplete since 0.007% donors (1 out of 15,000 blood donations) are not tracked down.

  4. Prevalence of human T-cell lymphotropic virus types 1 and 2 in blood donors of the Caruaru Blood Center (Hemope

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    Waleska Mayara Gomes de Lima

    2013-01-01

    Full Text Available BACKGROUND: There is difficulty in gathering data on the prevalence of human T-cell lymphotropic virus in blood donors as confirmatory testing is not mandatory in Brazil. This suggests there may be an underreporting of the prevalence. OBJECTIVE: To estimate the prevalence of human T-cell lymphotropic virus types 1 and 2 in donors of a blood bank in Caruaru, Brazil. METHODS: This was an observational, epidemiological, descriptive, longitudinal and retrospective study with information about the serology of donors of the Caruaru Blood Center, Fundação de Hematologia e Hemoterapia de Pernambuco (Hemope from May 2006 to December 2010. The data were analyzed using the Excel 2010 computer program (Microsoft Office(r. RESULTS: Of 61,881 donors, 60 (0.096% individuals were identified as potential carriers of human T-cell lymphotropic virus types 1 and 2. Of these, 28 (0.045% were positive and 32 (0.051% had inconclusive results in the serological screening. Forty-five (0.072% were retested; 17 were positive (0.027% and 3 inconclusive (0.005%. After confirmatory tests, 8 were positive (0.013%. Six (75% of the confirmed cases were women. CONCLUSION: Epidemiological surveys like this are very important in order to create campaigns to attract donors and reduce the costs of laboratory tests.

  5. [Profile of HIV infected patients among blood donors in Abidjan, Côte d'Ivoire (1992-1999)].

    Science.gov (United States)

    Minga, A K; Huët, C; Coulibaly, I; Abo, Y; Dohoun, L; Bonard, D; Gourvellec, G; Coulibaly, Y; Konaté, S; Dabis, F; Bondurand, A; Salamon, R

    2005-06-01

    The FonSIDA is a private clinic created in 1992 within the premises of the National Blood Transfusion Center of Abidjan (CNTS), the largest city in Côte d'Ivoire. It provides medical and psychological follow-up for blood donors which are diagnosed as HIV-infected. This Centre provides blood for transfusions in Abidjan and the surrounding area, which from 1992 to 1999 collected 263,398 blood units. In this period, 5574 subjects were detected HIV-positive. Among those, 1766 (32%) HIV infected blood donors came back to be tested for confirmation of HIV diagnosis. Since then, only 9% of the 5574 donors have been seen at least twice a year for medical and psychological follow-up. Women were more compliant than men in the FonSIDA Clinic: they constituted 62% of the 409 patients who were followed-up (p < 0.001). 53% of men had sex with prostitutes the year before HIV diagnosis. 67% of women stated voluntary abortion at least once. In the same period the systematic use of condoms was reported by only 7% of women and 5% of men. 22% of women and 28% of men reported having two or more sexual partners in the year before HIV diagnosis. The main aim of every blood center is to improve blood safety, particularly in developing countries. The appropriate counseling towards blood donors and especially those detected HIV positive can contribute to reduce new HIV infections in high HIV prevalence cities. Rate of compliance of HIV-infected patients to follow-up has risen to 11% in 1992-1994 to 60% in 1997-1999 and will contribute to reach this aim.

  6. Correlation of hepatitis C RNA and serum alanine aminotransferase in hepatitis B and C seronegative healthy blood donors

    OpenAIRE

    Ali Natasha; Moiz Bushra; Moatter Tariq; Ahmed Shiraz; Adil Salman; Khurshid Mohammad

    2010-01-01

    Introduction: Historically, serum alanine transaminase (ALT) has been used as a surrogate marker in the detection of hepatitis viruses in blood donors. With the availability of newer sensitive technologies for the detection of seroconversion, the value of ALT becomes questionable but continues to be used for this purpose with subsequent discarding of ALT elevated blood units. Objective: The present study aims to evaluate the significance and cost effectiveness of ALT as a surrogate marker for...

  7. 76 FR 51041 - Hemoglobin Standards and Maintaining Adequate Iron Stores in Blood Donors; Public Workshop

    Science.gov (United States)

    2011-08-17

    ... institutions, government agencies, and industry. Dates and Times: The public workshop will be held on November...). Nonetheless, some donors, especially repeat and premenopausal female donors, can develop iron deficiency,...

  8.  Glucose-6-Phosphate Dehydrogenase Deficiency among Male Blood Donors inSana’a City, Yemen

    Directory of Open Access Journals (Sweden)

    Molham AL-Habori

    2012-01-01

    Full Text Available  Objectives: To determine the prevalence of Glucose-6-phosphatedehydrogenase (G-6-PD deficiency among Yemeni people fromdifferent regions of the country living in the capital city, Sana’a,giving an indication of its overall prevalence in Yemen.Methods: A cross-sectional study was conducted among Yemenimale blood donors attending the Department of Blood Bank atthe National Centre of the Public Health Laboratories in thecapital city, Sana’a, Yemen. Fluorescent spot method was used forscreening, spectrophotometeric estimation of G-6-PD activityand separation by electrophoresis was done to determine the G-6-PD phenotype.Results: Of the total 508 male blood donors recruited into thestudy, 36 were G-6-PD deficient, giving a likely G-6-PD deficiencyprevalence of 7.1�20None of these deficient donors had history ofanemia or jaundice. Thirty-five of these deficient cases (97.2�howed severe G-6-PD deficiency class II (<10�0of normalactivity, and their phenotyping presumptively revealed a G-6-PDMediterraneanvariant.Conclusion: The results showed a significant presence of G-6-PD deficiency with predominance of a severe G-6-PD deficiencytype in these blood donors in Sana’a City, which could representan important health problem through occurrence of hemolyticanemia under oxidative stress. A larger sample size is needed todetermine the overall prevalence of G-6-PD deficiency, and shouldbe extended to include DNA analysis to identify its variants in Yemen.

  9. Seroprevalence of antibodies to hepatitis E virus in the normal blood donor population and two aboriginal communities in Malaysia.

    Science.gov (United States)

    Seow, H F; Mahomed, N M; Mak, J W; Riddell, M A; Li, F; Anderson, D A

    1999-10-01

    The prevalence of antibodies to hepatitis E virus (HEV) has been examined in many countries, but such studies have generally been limited to majority populations such as those represented in healthy blood donors or cross sections of urban populations. Due to its major route of enteric transmission, large differences in HEV prevalence might be expected between populations in the same country but with different living conditions. Using an ELISA based on GST-ORF2.1 antigen, the prevalence of IgG-class antibodies to HEV was examined in three distinct populations in Malaysia: the normal (urban) blood donor population and two aboriginal communities located at Betau, Pahang and Parit Tanjung, Perak. IgG anti-HEV was detected in 45 (44%) of 102 samples from Betau and 15 (50%) of 30 samples from Parit Tanjung, compared to only 2 (2%) of 100 normal blood donors. The distribution of sample ELISA reactivities was also consistent with ongoing sporadic infection in the aboriginal communities, while there was no significant relationship between HEV exposure and age, sex, or malaria infection. The high prevalence of antibodies to HEV in the two aboriginal communities indicates that this group of people are at high risk of exposure to HEV compared to the general blood donors, and the results suggest that studies of HEV seroprevalence within countries must take into account the possibility of widely varying infection rates between populations with marked differences in living conditions. PMID:10459151

  10. Donor lymphocyte infusions for the treatment of chronic myeloid leukemia relapse following peripheral blood or bone marrow stem cell transplantation

    NARCIS (Netherlands)

    Basak, G.W.; Wreede, L.C. de; Biezen, A. van; Wiktor-Jedrzejczak, W.; Halaburda, K.; Schmid, C.; Schaap, N.P.; Dazzi, F.; Borne, P.A. von dem; Petersen, E.; Beelen, D.; Abayomi, A.; Volin, L.; Buzyn, A.; Gurman, G.; Bunjes, D.; Guglielmi, C.; Olavarria, E.; Witte, T.J.M. de

    2013-01-01

    Peripheral blood used as a source of stem cells for transplantation (PBSCT) is known to exert stronger immune-mediated effects compared with BM (BMT). We decided to retrospectively analyze the impact of stem cell source on the OS of CML patients who relapsed after either matched related donor PBSCT

  11. Seroprevalence of antibodies to hepatitis E virus in the normal blood donor population and two aboriginal communities in Malaysia.

    Science.gov (United States)

    Seow, H F; Mahomed, N M; Mak, J W; Riddell, M A; Li, F; Anderson, D A

    1999-10-01

    The prevalence of antibodies to hepatitis E virus (HEV) has been examined in many countries, but such studies have generally been limited to majority populations such as those represented in healthy blood donors or cross sections of urban populations. Due to its major route of enteric transmission, large differences in HEV prevalence might be expected between populations in the same country but with different living conditions. Using an ELISA based on GST-ORF2.1 antigen, the prevalence of IgG-class antibodies to HEV was examined in three distinct populations in Malaysia: the normal (urban) blood donor population and two aboriginal communities located at Betau, Pahang and Parit Tanjung, Perak. IgG anti-HEV was detected in 45 (44%) of 102 samples from Betau and 15 (50%) of 30 samples from Parit Tanjung, compared to only 2 (2%) of 100 normal blood donors. The distribution of sample ELISA reactivities was also consistent with ongoing sporadic infection in the aboriginal communities, while there was no significant relationship between HEV exposure and age, sex, or malaria infection. The high prevalence of antibodies to HEV in the two aboriginal communities indicates that this group of people are at high risk of exposure to HEV compared to the general blood donors, and the results suggest that studies of HEV seroprevalence within countries must take into account the possibility of widely varying infection rates between populations with marked differences in living conditions.

  12. Comparison of risk factors among blood donors, volunteers and replacement individuals, infected or not by hepatitis C virus

    Directory of Open Access Journals (Sweden)

    MJDB Felippe

    2009-01-01

    Full Text Available Hepatitis C is transmitted primarily parenterally by contaminated blood and is often associated with: intravenous drug abuse, invasive procedures, blood transfusions, acupuncture, tattooing, and alcohol and tobacco use. This study aimed to quantify and evaluate the risk factors among blood donors, volunteer blood donors and replacement individuals, infected or not by the C virus. The main transmission routes of C virus were identified in 55 men and 25 women (GI monitored by the Ambulatory Unit of the Department of Tropical Diseases, Botucatu Medical School, and in 24 men and 26 women (GII, all active blood donors at the Bauru State Hospital Transfusional Agency. Both groups were similar in: tobacco and alcohol consumption, sexual behavior, tattooing and illicit drug use. The duration of alcohol and tobacco consumption and blood transfusions in GI were longer, whereas the option for steady partners, condom use, disposable materials and piercings were predominant in GII. In conclusion, the risk factors for hepatitis C demonstrate the necessity of health policies that act on the primary and secondary prevention levels (respectively, reduction of infection incidence and hepatopathy risk.

  13. 无偿献血者人类免疫缺陷病毒感染分析%HIV infection in voluntary blood donors

    Institute of Scientific and Technical Information of China (English)

    朱守兵; 刘祎; 李素娥; 傅立强; 钦博

    2015-01-01

    OBJECTIVE To understand the condition of HIV infection in voluntary blood donors so as to provide theoretical basis for improving the work of blood donor recruitment .METHODS The HIV screening results of 221 715 voluntary blood donors in Shaoxing from the year 2009 to 2013 were analyzed ,the information of those who were confirmed to have HIV infection was also investigated .The software SPSS 17 .0 was used for statistical anal‐ysis .RESULTS The positive rate of screening test on HIV in voluntary blood donors of Shaoxing from the year 2009 to 2013 was 1 .27‰ ,17 blood donors were confirmed to have HIV infection ,the rate was 7 .67/100000 . They were all anti‐HIV‐1 positive ,most of whom were unmarried (58 .82% ) ,aged 19 to 35 years (88 .24% ) , with high school education or lower (70 .59% ) and donated blood for the first time (70 .59% ) ,male (88 .24% ) . Among them ,the amount of natives was close to foreigners ,mainly including voluntary donors who donated at street spots and donation stations .Nine of them were traced as sexual transmission ,and male homosexual trans‐mission accounted for a large proportion .CONCLUSION The positive rate of screening test and confirmation test on HIV in voluntary blood donors of Shaoxing from the year 2009 to 2013 was low and the mode of individual blood donation at street spots was not safer than the mode of organized donation in units and institutes .The consultation before blood donation needs to be further enhanced .%目的:了解无偿献血者人类免疫缺陷病毒(HIV )感染状况,为更好的做好献血招募工作提供理论基础。方法对2009-2013年221715名无偿献血者抗‐HIV筛查结果进行分析,对确认为阳性者的资料进行回顾性研究,数据采用SPSS 17.0软件进行统计分析。结果2009-2013年绍兴地区221715名无偿献血者抗‐HIV初筛阳性率为1.27‰,经确认为阳性的有17人,确认后阳性率7.67/10万;

  14. Blood transfusion and iatrogenic risks in Mexico city: anti-Trypanosoma cruzi seroprevalence in 43,048 blood donors, evaluation of parasitemia, and electrocardiogram findings in seropositive

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    Nidia Hernández-Becerril

    2005-04-01

    Full Text Available Iatrogenous transmission of Trypanosoma cruziby blood transfusion was suggested as a potential risk by Pellegrino (1949. Seropositive blood donors in Mexico were first reported in 1978, however, limited information is available due to small sampling, the use of heterogeneous serologic assays, and geographically limited studies. A wide survey carried out in 18 out of the 32 states of Mexico, showed a national mean of 1.6% seropositive among 64,969 donors, ranging from 0.2 to 2.8%. In the present study, we have screened 43,048 voluntary blood donors in a period of five years at the Instituto Nacional de Cardiología I. Chávez, a concentration hospital located in Mexico city which serves mainly the metropolitan area and accepts from all over the country. Standardized ELISA and IIF were used to identify seropositive individuals in addition to hemoculture, PCR and standard 12 lead ECG tests that were applied to a group of seropositive patients (29/161. The result showed a seropositivity of 0.37% (161/43,048. From the group of seropositive individuals 40% (12/29 were potential carriers of T. cruzi at the donation time and 5/29 had subclinical ECG abnormalities. Parasitological tests performed in 70 erythrocyte and platelet fractions from seropositive units (70/161 showed negative results. Our findings strongly support T. cruzi screening in the transfusion medicine practice and identify subclinical heart disease among seropositive blood donors.

  15. Genetic Diversity of Recently Acquired and Prevalent HIV, Hepatitis B Virus, and Hepatitis C Virus Infections in US Blood Donors

    Science.gov (United States)

    Slikas, Elizabeth; Stramer, Susan L.; Kamel, Hany; Kessler, Debra; Krysztof, David; Tobler, Leslie H.; Carrick, Danielle M.; Steele, Whitney; Todd, Deborah; Wright, David J.; Kleinman, Steven H.; Busch, Michael P.

    2012-01-01

    (See the editorial commentary by Katz, on pages 867–9 and see the article by Stramer et al, on pages 886–94.) Background. Genetic variations of human immunodeficiency virus (HIV), hepatitis C virus (HCV), and hepatitis B virus (HBV) can affect diagnostic assays and therapeutic interventions. Recent changes in prevalence of subtypes/genotypes and drug/immune-escape variants were characterized by comparing recently infected vs more remotely infected blood donors. Methods. Infected donors were identified among approximately 34 million US blood donations, 2006–2009; incident infections were defined as having no or low antiviral antibody titers. Viral genomes were partially sequenced. Results. Of 321 HIV strains (50% incident), 2.5% were non-B HIV subtypes. Protease and reverse transcriptase (RT) inhibitor resistance mutations were found in 2% and 11% of infected donors, respectively. Subtypes in 278 HCV strains (31% incident) yielded 1a>1b>3a>2b>2a>4a>6d, 6e: higher frequencies of 3a in incident cases vs higher frequencies of 1b in prevalent cases were found (P = .04). Twenty subgenotypes among 193 HBV strains (26% incident) yielded higher frequencies of A2 in incident cases and higher frequencies of A1, B2, and B4 in prevalent cases (P = .007). No HBV drug resistance mutations were detected. Six percent of incident vs 26% of prevalent HBV contained antibody neutralization escape mutations (P = .01). Conclusions. Viral genetic variant distribution in blood donors was similar to that seen in high-risk US populations. Blood-borne viruses detected through large-scale routine screening of blood donors can complement molecular surveillance studies of highly exposed populations. PMID:22293432

  16. Tabagismo e obesidade abdominal em doadores de sangue Smoking and abdominal fat in blood donors

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    Cássia da Silva Faria

    2012-06-01

    Full Text Available OBJETIVO: Analisar a associação entre tabagismo e obesidade abdominal em doadores de sangue. MÉTODOS: Estudo de corte transversal com 1.235 homens adultos doadores de sangue (idade: 20-59 anos em Cuiabá (MT. Foram coletados dados socioeconômicos, demográficos e antropométricos, bem como informações sobre o estilo de vida dos participantes. Neste estudo, a circunferência da cintura e a relação cintura/quadril foram utilizadas como marcadores de obesidade abdominal. A associação desses dois marcadores com o tabagismo foi analisada por meio de regressão linear múltipla em modelos distintos, ajustados para potenciais fatores de confusão. RESULTADOS: Dos 1.235 entrevistados, 273 (22,1% declararam ser fumantes e, desses, 99 (36,3% relataram fumar mais de 15 cigarros por dia. A média do índice de massa corpórea nos fumantes foi menor que nos não fumantes (p 11 cigarros/dia. CONCLUSÕES: Nesta amostra, o tabagismo associou-se positivamente com indicadores de obesidade abdominal, independentemente de potenciais fatores de confusão, inclusive o consumo de bebidas alcoólicas.OBJECTIVE: To assess the association between smoking and abdominal fat among male blood donors. METHODS: This was a cross-sectional study involving 1,235 adult male blood donors (age, 20-59 years in the city of Cuiabá, Brazil. Socioeconomic, demographic, and anthropometric data, as well as information on the lifestyle of the participants, were collected. In this study, waist circumference and waist-to-hip ratio were used as markers of abdominal fat. The association between these two markers and smoking was analyzed by multiple linear regression in separate models, adjusted for potential confounders. RESULTS: Of the 1,235 respondents, 273 (22.1% reported being smokers, and, of those, 99 (36.3% reported smoking more than 15 cigarettes per day. The average body mass index was lower among smokers than among nonsmokers (p 11 cigarettes/day. CONCLUSIONS: In our sample

  17. Investigation on HIV infections in voluntary blood donors in Chongqing%重庆市无偿献血人群HIV感染情况的调查分析

    Institute of Scientific and Technical Information of China (English)

    唐卫国; 廖红文; 段恒英; 黄霞; 汪娟; 王珍贤

    2011-01-01

    Objective To develop suitable strategy for blood safety under new situations, HIV prevalence in voluntary blood donors in Chongqing was analyzed. Methods Information of HIV infected individuals from voluntary blood donors from Chongqing Blood Center between 2000 and 2009 was collected,the epidemiological characteristics of those during 2007~2009 were analyzed.Results HIV infections among voluntary blood donors were increasing in Chongqing in recent years,the HIV infection rate was 72.1 per 100,000,most HIV infected individuals were males of less than 30 years old;Most of them were infected only by HIV,others were coinfected with TP, HCV, HBV. No difference was observed between first time and repeat voluntary blood donors.Conclusion Based on current situation of HIV infections in voluntary blood donors in Chongqing,health assessments of blood donation and consultations prior to donation should be strengthened,identification for high risk donors should be enhanced,health education for voluntary blood donation in publicity would be required , and development of stable low risk blood donation teams should be recommended.%目的 通过分析重庆市无偿献血者人类免疫缺陷病毒(HIV)感染的情况,探寻如何保证新形势下血液安全的策略.方法 按血站常规检测献血者HIV感染状况,收集2000~2009年重庆市血液中心献血者HIV感染资料,重点分析2007~2009年间HIV感染者基本信息,分析其流行病学特征.结果 重庆市无偿献血人群的HIV感染率呈现逐年上升趋势,2009年HIV感染率达72.1/10万;HIV感染者主要为小于30岁的低年龄段男性;多数为单一性感染,其次为合并梅毒螺旋体、丙型肝炎病毒(HCV)、乙型肝炎病毒(HBV)等病原体感染.在初次献血和再次献血的无偿献血者中,HIV感染率差异无统计学意义(P>0.05).结论 针对重庆市无偿献血者HIV感染的现状,应加强献血的健康评估和献血前咨询工作,加强对高危献血者

  18. A Randomized Control Study to Evaluate Effects of Short-term Oral Iron Supplementation in Regular Voluntary Blood Donors.

    Science.gov (United States)

    Dara, Ravi Chandrabhan; Marwaha, Neelam; Khetan, Dheeraj; Patidar, Gopal Kumar

    2016-09-01

    Regular blood donation can lead to iron deficiency anaemia. Early recognition and reversal of excessive iron loss by iron supplementation may avoid symptomatic iron store depletion in blood donors. The aim of this study was to assess the efficacy of iron supplementation in maintaining the iron stores of voluntary blood donors. A total of 200 regular volunteers who donated twice in previous year were randomly divided into two groups. Iron: oral iron supplementation tablets of elemental iron as ferrous fumarate. Placebo group: glucose containing capsules, to be taken once daily for 21 days after one unit of blood donation. Their hemogram, serum ferritin, red cell indices and red cell distribution width were determined at baseline and after 1 month and at the time of next blood donation. Out of 200 volunteers enrolled 98 were assigned to iron group and rest 102 into placebo group. Total of 37 % donors dropped out, yielding a dropout rate of 35 % in iron group and 39 % in the placebo group. The haemoglobin and ferritin levels showed significant improvement in iron group compared to placebo group (p donation but was not sufficient to sustain the iron stores over a period of 3 months. Thus there is need to evaluate increased dosage or duration of iron supplementation in maintaining the iron stores. PMID:27429522

  19. Prevalence and genotyping of hepatitis C virus in blood donors in the state of Pará, Northern Brazil

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    Aldemir B Oliveira-Filho

    2010-02-01

    Full Text Available Given the scarcity of epidemiological information on hepatitis C virus (HCV infection in Northern Brazil, we determined the prevalence and genotypic frequency in blood donors in the state of Pará (PA. Blood samples from all of the blood donors at the Fundação HEMOPA (blood bank of PA from 2004-2006 were screened for the presence of antibodies to anti-HCV and samples seroreactive to anti-HCV were further tested for HCV RNA using real-time PCR. In total, 116 HCV-RNA samples were genotyped, based on maximum likelihood phylogenetic analyses, using BioEdit, Modelgenerator, PHYML and FigTree software. The population consisted of 242,726 volunteers who donated blood from 2004-2006; the most common subgroup was males between the ages of 18-29 years old (37.30%. Within the whole group, 1,112 blood donors (0.46% had indeterminate or positive serology; among these, 28.78% were males whose ages ranged from 18-29 years. A diagnosis of chronic HCV infection was confirmed for 304 donors (60.20% males; 66.45% were 30-49 years old, resulting in a prevalence of HCV RNA in 0.13% of the samples (304 of 242,726. HCV genotyping revealed a high frequency of genotype 1 (108/116 followed by genotype 3 (8/116. This study found HCV infection to be relatively infrequent in PA; genotype 1 was most commonly isolated. This information can help guide prevention and control policies aimed at efficient diagnosis and control measures.

  20. Prevalence and prevalence trends of transfusion transmissible infections among blood donors at four chinese regional blood centers between 2000 and 2010

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    Li Changqing

    2012-08-01

    Full Text Available Abstract Background In China, high prevalence of HBV and HCV parallels with the growing epidemic of syphilis and HIV in the general population poses a great threat to blood safety. This study investigated the prevalence of serologic markers for transfusion transmissible infections (TTIs among four Chinese blood centers. Methods We examined whole blood donations collected from January 2000 through December 2010 at four Chinese blood centers. Post-donation testing of TTIs (HIV, HBV, HCV and syphilis were conducted using two different enzyme-linked immunosorbent assay kits for each seromarker. The prevalence of serologic markers for TTIs (% was calculated and additional analysis was conducted to examine donor characteristics associated with positive TTIs serology. Results Of the 4,366,283 donations, 60% were from first-time donors and 40% were from repeated donors. The overall prevalence of HIV, HBsAg, HCV and syphilis was 0.08%, 0.86%, 0.51% and 0.47%, respectively. The prevalence profile of TTIs varied among different blood centers and appeared at relatively high levels. Overall, the prevalence of HBsAg and HCV demonstrated a decline trend among four blood centers, while the prevalence of HIV and syphilis displayed three different trends: constantly steady, continually increasing and declining among different centers. Conclusions This study reflects the risk of TTIs has been greatly reduced in China, but blood transfusion remains an ongoing risk factor for the spread of blood-borne infections, and further work and improvements are needed to strengthen both safety and availability of blood in China.

  1. 献血人群红细胞血型意外抗体的筛选%Screening unexpected antibody of red cells blood group in the blood donors

    Institute of Scientific and Technical Information of China (English)

    祝宏; 洪小珍; 吴亚玲; 励晓涛; 马开荣; 兰小飞; 朱发明

    2012-01-01

    目的:分析献血人群中红细胞血型意外抗体的情况,并对抗体特性进行确认.方法:应用O型红细胞结合PK7200血型检测系统筛选红细胞血型意外抗体,利用谱细胞鉴定抗体特性.结果:在献血人群中红细胞血型意外抗体阳性率为0.025%,意外抗体以抗M和冷凝集素为主.发现两例类孟买型血型和两例p血型.结论:献血人群中存在低比例的红细胞血型意外抗体,在献血者血型检测中应加以重视.%Objective;To determine unexpected antibody of red cells blood group in the blood donors and identify the characteristics of the antibody. Methods: The unexpected antibody was screened by 0 blood group cells and PK7200 system. Characteristics of the antibody were identified by panel cells.. Results: The prevalence rate of unexpected antibody was 0.025% in the blood donors. The common unexpected antibodies were anti - M and cold ag-glutinin. Two cases with para - Bombay phenotype and two cases with p phenotype were found. Conclusion; Low prevalence of unexpected antibody existed in the blood donors. It is important to screen unexpected antibody in donor's blood grouping.

  2. Ethnicity, education, and blood pressure in Cuba.

    Science.gov (United States)

    Ordunez, Pedro; Munoz, Jose Luis Bernal; Espinosa-Brito, Alfredo; Silva, Luis Carlos; Cooper, Richard S

    2005-07-01

    The causes of variation in hypertension risk by ethnicity and educational level are not well understood. To gain further insight into this issue in a nonindustrialized country, a population-based sample of 1,667 persons aged 15-74 years was recruited in Cienfuegos, Cuba. In this 2001-2002 study, interviewers classified 29% of participants as Black or mulatto and 71% as White. Educational attainment was stratified at the median number of school years. Compared with White women, non-White women had higher blood pressures (3.0/1.7, systolic blood pressure/diastolic blood pressure) and a higher prevalence of hypertension (24%, 95% confidence interval: 20, 28 vs. 15%, 95% confidence interval: 12, 18). Among men, no differences in blood pressure were observed by ethnicity. Men with a lower level of education had a 14% lower risk of hypertension compared with men above the median. However, women with a lower level of education had a 24% increase in risk. The effect of education was equally strong among Whites alone and when occupation was used for stratification. No variation was observed for body mass index or self-reported health behaviors by ethnicity or education. The narrower ethnic gradient in hypertension prevalence than seen in North America and the gender-specific social status effect, in the context of relatively equal living conditions, suggest that the influence of psychosocial stressors may be specific to cultural contexts.

  3. Determination of HBV Genotypes among Hbs Ag Positive Blood Donors in Tehran, Iran Using PCR-RFLP

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    S Milani

    2009-03-01

    Full Text Available Background: Hepatitis B virus (HBV is one of the major causative agents of acute and chronic liver disease worldwide and is believed to be responsible for a million deaths annually. On the basis of a comparison of complete genomic se­quences, HBV has been classified into eight genotypes A-H which show a geographical distribution. Some genotypes are associ­ated with different clinical outcomes. Identification of HBV genotypes is important to begin and follow up the treat­ment."nMethods: In this cross-sectional study, the serum samples of blood donors were collected from Tehran Blood Transfusion Cen­ters in period during "2005-2006". Sera of 55 blood donors who were positive for hepatitis B surface antigen were se­lected. DNA was extracted using commercial kit and the S gene sequence was amplified by nested-PCR. PCR products were then analyzed for restriction enzymes that would be genotype specific."nResults: Genotype D was found the only type in all HBV DNA positive serum samples, in Tehran."nConclusion: Genotype D is dominant among Tehran's blood donors, which is consistent with Iran and the Middle East domi­nant genotype.  

  4. PREVENTION OF POST-TRANSFUSION HEPATITIS BY SCREENING OF ANTIBODY TO HEPATITIS B CORE ANTIGEN IN HEALTHY BLOOD DONORS

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    Sudha Bhat

    2011-01-01

    Full Text Available

    Background: Transfusion-associated hepatitis B viral infection continues to be a major problem in India even after adoption of mandatory screening for HBsAg by ELISA method. The high incidence of TAHBV is reported in patients receiving multiple transfusions.

    Objective: To study the seroprevalence of hepatitis B core antibody among healthy voluntary blood donors

    Subjects and Methods: The study was conducted in the department of Transfusion Medicine of a tertiary care referral hospital. A total of 12,232 volunteers after passing through the stringent criteria were selected for blood donation. Donor samples were tested for all mandatory transfusion transmissible infections and anti HBc IgM (Monolisa HBc IgM PLUS:BIO-RAD, France. Reactive results were confirmed by repeat testing in duplicate. Donor data was analyzed using SPSS software and Chi-square test was used to calculate the significance of difference between the groups.

    Results:A total of 12,232 healthy voluntary blood donors were recruited. Majority (93.4% were males. Median age of donor population was 26 years (range: 18-60 years. Eighty six (0.7% were positive for HBsAg, which comes under “low prevalence (<2% zone” as per WHO. On screening for HBcAg Ig M, 15 (0.1% were found to be positive and none were HBsAg reactive. There was no significance of difference in the mean age between reactive and non-reactive donors.

    Conclusion:Evaluating the usefulness of anti-HBc screening is critical. Anti HBcAg IgM screening may be included in routine screening of donors as it is an indicator of occult HBV during window period. The cost and the unnecessary wastage of the blood units when they are positive for anti HBsAg along with the core antibody need to be studied.

     

  5. Individual donor-nucleic acid testing for human immunodeficiency virus-1, hepatitis C virus and hepatitis B virus and its role in blood safety

    OpenAIRE

    Rajesh Kumar; Sonia Gupta; Amarjit Kaur; Manvi Gupta

    2015-01-01

    Background: Transfusion-transmitted infections (TTIs) are one of the biggest threats to blood transfusion safety. Nucleic acid testing (NAT) in blood donor screening has been implemented in many countries to reduce the risk of TTIs. NAT shortens this window period, thereby offering blood centers a much higher sensitivity for detecting viral infections. Aims: The objective was to assess the role of individual donor-NAT (ID-NAT) for human immunodeficiency virus-1 (HIV-1), hepatitis C virus (HCV...

  6. 互助献血与无偿献血血清学标志物对比分析%The analysis of serological markers among mutual blood donors and voluntary blood donors

    Institute of Scientific and Technical Information of China (English)

    陈志忠; 张欣; 陈尚良; 廖扬勋; 梁洁贞; 陈超红; 陆倩文

    2014-01-01

    目的:对互助献血和无偿献血的血清学指标进行分析对比,研究探讨互助献血的风险。方法2006年1月至2012年12月,对符合献血条件的1834例互助献血者以及217323例无偿献血者进行了血液采集和留取 ETDA 抗凝样本,进行 ALT、HBsAg、Anti-HCV、Anti-HIV、梅毒共5个项目的初复检。结果互助献血(8.67%)的总阳性率要高于无偿献血组(6.31%),但两组的ALT 阳性率差异无统计学意义,互助献血组的HBsAg、Anti-HCV、梅毒均高于无偿献血组。结论无偿献血群体的血清学安全指标优于互助献血组,是血源性传播疾病较低的低危人群,应该是血液供应的主要来源群体;对互助献血采取合理的干预措施后,能确保其安全指数与无偿献血等同,可作为无偿献血的有益补充。%Objectives To analyze the epidemiology of viral markers and ALT among volunteer and replacement donors, and to reevaluate the risk of mutual blood donors. Methods From 2006, January to 2012, December, replacement and voluntary donors were recruited by hospital and center blood station respectively, and all the donors were screened HBsAg, anti-HCV, anti-HIV (1 + 2), syphilis and ALT in center blood station twice. Results In 217 323 volunteers and 1 834 replacement donors, the total seroprevalence was higher in replacement group than in voluntary group (8.67% vs 6.31%), but the prevalence of ALT were not significantly differences. The prevalence of HBsAg, anti-HCV and syphilis were higher in replacement group than in voluntary group. Conclusions Generally, volunteers cloud provide more viral safety than replacement donors, who should be the main source of blood supply. But with appropriate intervention measures, such as pre-donor screening and other donor selection policy, replacement donors and voluntary donors provide a similar level of viral safety.

  7. Prevention of Post-Transfusion Hepatitis by Screening of Antibody to Hepatitis B Core Antigen in Healthy Blood Donors

    OpenAIRE

    Shastry, S.; S S Bhat

    2011-01-01

    Background Transfusion-associated hepatitis B viral infection continues to be a major problem in India even after adoption of mandatory screening for HBsAg by ELISA method. The high incidence of TAHBV is reported in patients receiving multiple transfusions. Objective To study the seroprevalence of hepatitis B core antibody among healthy voluntary blood donors Subjects and Methods The study was conducted in the department of Transfusion Medicine of a tertiary care referral hospital. A total of...

  8. PREVENTION OF POST-TRANSFUSION HEPATITIS BY SCREENING OF ANTIBODY TO HEPATITIS B CORE ANTIGEN IN HEALTHY BLOOD DONORS

    OpenAIRE

    Shamee Shastry; Sudha Bhat

    2011-01-01

    Background: Transfusion-associated hepatitis B viral infection continues to be a major problem in India even after adoption of mandatory screening for HBsAg by ELISA method. The high incidence of TAHBV is reported in patients receiving multiple transfusions. Objective: To study the seroprevalence of hepatitis B core antibody among healthy voluntary blood donors Subjects and Methods: The study was conducted in the department of Transfusion Medicine of a tertiary care referral h...

  9. Exposure to vector-borne pathogens in candidate blood donor and free-roaming dogs of northeast Italy

    OpenAIRE

    Vascellari, Marta; Ravagnan, Silvia; Carminato, Antonio; Cazzin, Stefania; Carli, Erika; Da Rold, Graziana; Lucchese, Laura; Natale, Alda; Otranto, Domenico; Capelli, Gioia

    2016-01-01

    Background Many vector-borne pathogens including viruses, bacteria, protozoa and nematodes occur in northeast Italy, representing a potential threat to animal and human populations. Little information is available on the circulation of the above vector-borne pathogens in dogs. This work aims to (i) assess exposure to and circulation of pathogens transmitted to dogs in northeast Italy by ticks, sandflies, and mosquitoes, and (ii) drive blood donor screening at the newly established canine bloo...

  10. The Use of Back-up Units to Enhance the Safety of Unrelated Donor Cord Blood Transplantation

    OpenAIRE

    Ponce, Doris M.; Lubin, Marissa; Gonzales, Anne Marie; Byam, Courtney; Wells, Deborah; Ferrante, Rosanna; Heller, Glenn; Giralt, Sergio; Papadopoulos, Esperanza B.; Kernan, Nancy A.; Scaradavou, Andromachi; Barker, Juliet N.

    2012-01-01

    The inability to obtain additional stem cells is a disadvantage of unrelated donor cord blood (CB) transplantation (CBT). Moreover, in the event of problems with unit shipment, compromised unit quality, thaw mishaps, or graft failure, the time to secure a back-up graft could be unacceptable. Emergent shipment of 1-2 back-up units that have been previously typed and reserved could overcome this limitation. However, the advantages of this approach are not established. Therefore, we present our ...

  11. Russia as a Returning Donor--Four Roles in Development Assistance to Education

    Science.gov (United States)

    Piattoeva, Nelli; Takala, Tuomas

    2015-01-01

    This article analyses the role of Russia in development assistance to education in light of the changing architecture of development assistance--the variety of positions identified with both "new" and "old" donor countries. We shed light on Russia's aims and agenda in the field of development assistance in general and…

  12. An Emerging Donor in Education and Development: A Case Study of China in Cameroon

    Science.gov (United States)

    Nordtveit, Bjorn H.

    2011-01-01

    This paper analyzes China's new approaches of education aid to Africa through a case study of Cameroon. China's cooperation has been characterized by different discourses and different historic relationships with recipient countries than those of traditional donors. Sino-African policies have gone through different stages, each connected to wider…

  13. [The biological significance of the genetically determined Se-se human blood group and its effect on the antibody formation process in donors immunized with staphylococcal anatoxin].

    Science.gov (United States)

    Patoka, V V

    1999-01-01

    82 blood donors have been observed, 63 of them were immunized. Blood group ABO(H), secreting group Se--se and Staphylococcus antibody contents (anti-alpha-staphylolysins) were determined in all the donors. It was found out that the donors-secretors with A(II) blood group exhibited the antibody-production increasing. It is supposed that the secreting of group-specific substance A, that has structural elements similar those of staphylococcus into saliva promotes antibody production increase against staphylococcus. The mechanism of such specific stimulation remains to be unknown and requires further studying. PMID:10687067

  14. Presence of anti-Leishmania (Viannia braziliensis antibodies in blood donors in the West-Central region of the State of Paraná, Brazil

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    Lais de Souza Braga

    2015-10-01

    Full Text Available ABSTRACTINTRODUCTION:Serological screening in blood banks does not include all transmittable diseases. American cutaneous leishmaniasis (ACL has a high detection rate in the municipalities of the State of Paraná.METHODS:This study analyzed the presence of anti- Leishmania braziliensisantibodies in 176 blood donors who live in these endemic areas. The variables were analyzed with the χ2 test and Stata 9.1 software. RESULTS: Twenty (11.4% samples were positive for the presence of anti- L. braziliensisantibodies. CONCLUSIONS: The high percentage of donors with anti- Leishmania spp. antibodies indicates the need to study the risk of ACL transmission through blood donors.

  15. Seroprevalence and Associated Risk Factors for Toxoplasma gondii Infection in Healthy Blood Donors: A Cross-Sectional Study in Sonora, Mexico

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    Cosme Alvarado-Esquivel

    2016-01-01

    Full Text Available Toxoplasma gondii (T. gondii can be transmitted by blood transfusion. We determined the prevalence of T. gondii infection in healthy blood donors in Hermosillo city, Mexico, and the association of infection with T. gondii with the sociodemographic, clinical, and behavioral characteristics of blood donors. Four hundred and eight blood donors who attended two public blood banks in Hermosillo city were examined for anti-T. gondii IgG and IgM antibodies by using enzyme-linked immunoassays. Of the 408 blood donors (mean age 31.77±9.52; range 18–60 years old studied, 55 (13.5% were positive for anti-T. gondii IgG antibodies, and 12 (21.8% of them were also positive for anti-T. gondii IgM antibodies. Multivariate analysis showed that seropositivity to T. gondii was associated with age (OR = 1.74; 95% CI: 1.03–2.94; P=0.03 and tobacco use (OR = 2.09; 95% CI: 1.02–4.29; P=0.04. Seropositivity to T. gondii was correlated with the number of pregnancies, deliveries, and cesarean sections. The seroprevalence of T. gondii infection in blood donors in Sonora is the highest reported in blood donors in northern Mexico so far. This is the first report of an association of T. gondii exposure and tobacco use. Further research to confirm this association is needed.

  16. Seroprevalence and Associated Risk Factors for Toxoplasma gondii Infection in Healthy Blood Donors: A Cross-Sectional Study in Sonora, Mexico

    Science.gov (United States)

    Alvarado-Esquivel, Cosme; Rascón-Careaga, Antonio; Hernández-Tinoco, Jesús; Corella-Madueño, María Alba Guadalupe; Sánchez-Anguiano, Luis Francisco; Aldana-Madrid, María Lourdes; Velasquez-Vega, Edgar; Quizán-Plata, Trinidad; Navarro-Henze, José Luis; Badell-Luzardo, Joel Alberto; Gastélum-Cano, José María; Liesenfeld, Oliver

    2016-01-01

    Toxoplasma gondii (T. gondii) can be transmitted by blood transfusion. We determined the prevalence of T. gondii infection in healthy blood donors in Hermosillo city, Mexico, and the association of infection with T. gondii with the sociodemographic, clinical, and behavioral characteristics of blood donors. Four hundred and eight blood donors who attended two public blood banks in Hermosillo city were examined for anti-T. gondii IgG and IgM antibodies by using enzyme-linked immunoassays. Of the 408 blood donors (mean age 31.77 ± 9.52; range 18–60 years old) studied, 55 (13.5%) were positive for anti-T. gondii IgG antibodies, and 12 (21.8%) of them were also positive for anti-T. gondii IgM antibodies. Multivariate analysis showed that seropositivity to T. gondii was associated with age (OR = 1.74; 95% CI: 1.03–2.94; P = 0.03) and tobacco use (OR = 2.09; 95% CI: 1.02–4.29; P = 0.04). Seropositivity to T. gondii was correlated with the number of pregnancies, deliveries, and cesarean sections. The seroprevalence of T. gondii infection in blood donors in Sonora is the highest reported in blood donors in northern Mexico so far. This is the first report of an association of T. gondii exposure and tobacco use. Further research to confirm this association is needed. PMID:27446960

  17. Seroprevalence and Associated Risk Factors for Toxoplasma gondii Infection in Healthy Blood Donors: A Cross-Sectional Study in Sonora, Mexico.

    Science.gov (United States)

    Alvarado-Esquivel, Cosme; Rascón-Careaga, Antonio; Hernández-Tinoco, Jesús; Corella-Madueño, María Alba Guadalupe; Sánchez-Anguiano, Luis Francisco; Aldana-Madrid, María Lourdes; Velasquez-Vega, Edgar; Quizán-Plata, Trinidad; Navarro-Henze, José Luis; Badell-Luzardo, Joel Alberto; Gastélum-Cano, José María; Liesenfeld, Oliver

    2016-01-01

    Toxoplasma gondii (T. gondii) can be transmitted by blood transfusion. We determined the prevalence of T. gondii infection in healthy blood donors in Hermosillo city, Mexico, and the association of infection with T. gondii with the sociodemographic, clinical, and behavioral characteristics of blood donors. Four hundred and eight blood donors who attended two public blood banks in Hermosillo city were examined for anti-T. gondii IgG and IgM antibodies by using enzyme-linked immunoassays. Of the 408 blood donors (mean age 31.77 ± 9.52; range 18-60 years old) studied, 55 (13.5%) were positive for anti-T. gondii IgG antibodies, and 12 (21.8%) of them were also positive for anti-T. gondii IgM antibodies. Multivariate analysis showed that seropositivity to T. gondii was associated with age (OR = 1.74; 95% CI: 1.03-2.94; P = 0.03) and tobacco use (OR = 2.09; 95% CI: 1.02-4.29; P = 0.04). Seropositivity to T. gondii was correlated with the number of pregnancies, deliveries, and cesarean sections. The seroprevalence of T. gondii infection in blood donors in Sonora is the highest reported in blood donors in northern Mexico so far. This is the first report of an association of T. gondii exposure and tobacco use. Further research to confirm this association is needed.

  18. Impacto da doação de sangue nos depósitos de ferro do organismo de doadores Impact of blood donation on donor iron reserves

    Directory of Open Access Journals (Sweden)

    Rita C. Mousinho-Ribeiro

    2008-02-01

    Full Text Available Iron is a vital element in the human metabolism. It plays a central role in erythropoiesis and is also involved in many other intracellular processes in all the tissues of the body. Blood donation results in a substantial (200 to 250 ng loss of iron at each donation (425 to 475 ml with subsequent mobilization of iron from body deposits. Repetitive donations of blood my cause the depletion of iron reserves in blood donors and thus cause health disorders. Recent reports have shown that iron reserves are generally small and iron depletion is more common in blood donors than in non-donors. The high frequency of iron deficiency in blood donors reported by these studies suggests a need for more accurate studies, as measurement of hemoglobin and hematicrit alone is insufficient to identify and exclude prospective blood donors with iron deficiency but without anemia. It is important, therefore, that blood banks evaluate the risk-benefit of implanting tests to analyze organism iron reserves such as the measurement of serum ferritin of all individuals who donate more than three times per year in order to make the blood donation process safer for both donors and transfused patients.

  19. A Family Cluster of Chagas Disease Detected through Selective Screening of Blood Donors: A Case Report and Brief Review

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    Guillaume Mongeau-Martin

    2015-01-01

    Full Text Available Chagas disease (CD is a protozoan infection caused by Trypanosoma cruzi, which is transmitted by triatomine insect vectors in parts of Latin America. In a nonendemic country, such as Canada, spread can still occur via vertical transmission, and infected blood or organ donations. The Canadian Blood Services and Héma-Québec have both implemented selective screening of blood donors for CD based on risk factors. In 2011, Héma-Québec identified two seropositive ‘at-risk’ Chilean siblings who had donated blood in Montreal, Quebec. They were referred to the JD MacLean Centre for Tropical Diseases (Montreal, Quebec for confirmatory testing (T cruzi excreted-secreted antigen ELISA, polymerase chain reaction and/or radioimmunoprecipitation assay and follow-up. Screening of the rest of the family revealed two other seropositive family members (the mother and sister. While their geographical history in Chile suggests vectorial transmission, this family cluster of CD raises the possibility of vertical transmission. Congenital infection should always be considered among CD-positive mothers and pregnant women. With blood donor screening, Canadian physicians will increasingly see patients with CD and should know how to manage them appropriately. In addition to the case presentation, the authors review the transmission, screening and clinical management of CD in a nonendemic context.

  20. A family cluster of Chagas disease detected through selective screening of blood donors: A case report and brief review.

    Science.gov (United States)

    Mongeau-Martin, Guillaume; Ndao, Momar; Libman, Michael; Delage, Gilles; Ward, Brian J

    2015-01-01

    Chagas disease (CD) is a protozoan infection caused by Trypanosoma cruzi, which is transmitted by triatomine insect vectors in parts of Latin America. In a nonendemic country, such as Canada, spread can still occur via vertical transmission, and infected blood or organ donations. The Canadian Blood Services and Héma-Québec have both implemented selective screening of blood donors for CD based on risk factors. In 2011, Héma-Québec identified two seropositive 'at-risk' Chilean siblings who had donated blood in Montreal, Quebec. They were referred to the JD MacLean Centre for Tropical Diseases (Montreal, Quebec) for confirmatory testing (T cruzi excreted-secreted antigen ELISA, polymerase chain reaction and/or radioimmunoprecipitation assay) and follow-up. Screening of the rest of the family revealed two other seropositive family members (the mother and sister). While their geographical history in Chile suggests vectorial transmission, this family cluster of CD raises the possibility of vertical transmission. Congenital infection should always be considered among CD-positive mothers and pregnant women. With blood donor screening, Canadian physicians will increasingly see patients with CD and should know how to manage them appropriately. In addition to the case presentation, the authors review the transmission, screening and clinical management of CD in a nonendemic context. PMID:26236358

  1. Seroprevalence of HIV, HBV, HCV and Syphilis in Blood Donors in Saurashtra Region of Gujarat: Declining Trends Over a Period of 3½ Years

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    Dhruva Gauravi A

    2012-04-01

    Full Text Available Background: Transfusion of blood and blood products is a life saving intervention and benefits innumerous patients worldwide. At the same time it could be an ominous mode of infection transmission to recipients. In 15 percent of total patients infected with HIV, blood transfusion has been the responsible mechanism of transmission. Methods: In this study, we aimed to access the prevalence and trend of HIV, HBV, HCV and Syphilis over the last 3½ years (January 2008 to June 2011 among the blood donors who came to donate blood at Blood Bank, P.D.U. Medical College & Hospital, Rajkot as well as in various blood donation camps organized by the same blood bank. Results: From the total of 30,178 blood donors, 711 (2.35% had serological evidence of infection with at least one pathogen, either of HIV, HBV, HCV or Syphilis. These included 131 (0.43% with HIV, 293 (0.97% with HBV, 124 (0.41% with HCV and 94 (0.31% with Syphilis. Moreover, significantly declining trends of HIV, HBV and Syphilis was observed over the study period. Conclusion: A substantial percentage of blood donors harbor HIV, HBV, HCV and Syphilis infections. Strict selection of blood donors and comprehensive screening of donors’ blood using standard methods are highly recommended to ensure the safety of blood for recipient.

  2. Is having sex with other men a risk factor for transfusion-transmissible infections in male blood donors in Western countries? A systematic review.

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    Emmy De Buck

    Full Text Available Although increased prevalence of transfusion transmissible infections (TTI among "men who have sex with men" (MSM has been well documented, the exclusion of MSM as blood donors is contested. The aim of this systematic review is to find studies that describe the risk of TTI in MSM blood donors.We searched MEDLINE, Embase, The Cochrane Central Register of Controlled Trials, Cinahl, and Web of Science, and used GRADE for determining evidence quality. We included studies comparing MSM and non-MSM blood donors (or people eligible to give blood, living in areas most relevant for our Blood Service.Out of 18 987 articles, 14 observational studies were included. Two studies directly compared MSM with non-MSM donors showing that MSM donors have a statistically significant higher risk of HIV-1 infections. In one of these studies it was shown that this was related to recent (< 12 months MSM contact. In two additional studies no evidence was shown in favour of a certain deferral period for MSM. Ten studies, applying permanent deferral for MSM, compared infected versus non-infected donors. One study found that MSM is a statistically significant risk factor for HIV-1 infection in blood donors. For other TTI such as HBV or HCV, an increased risk of infection could not be demonstrated, because the precision of the results was affected by the low numbers of donors with MSM as risk factor, or because of risk of bias in the included studies. All studies included low level evidence, because of risk of bias and imprecision of the results.High-quality studies investigating the risk of TTI in MSM who donate blood are scarce. The available evidence suggests a link between MSM blood donors and HIV-1 infection, but is too limited to be able to unambiguously/clearly recommend a certain deferral policy.

  3. Preliminary evidence of a decline in perfluorooctanesulfonate (PFOS) and perfluorooctanoate (PFOA) concentrations in American Red Cross blood donors.

    Science.gov (United States)

    Olsen, Geary W; Mair, David C; Reagen, William K; Ellefson, Mark E; Ehresman, David J; Butenhoff, John L; Zobel, Larry R

    2007-05-01

    The purpose of this pilot study was to determine whether perfluorooctanesulfonate (PFOS,C(8)F(17)SO(3)(-)) and perfluorooctanoate (PFOA,C(7)F(15)CO(2)(-)) concentrations in American Red Cross blood donors from Minneapolis-St. Paul, Minnesota have declined after the 2000-2002 phase-out of perfluorooctanesulfonyl-fluoride (POSF, C(8)F(17)SO(2)F)-based materials by the primary global manufacturer, 3M Company. Forty donor plasma samples, categorized by age and sex, were collected in 2005, and PFOS and PFOA concentrations were compared to 100 (non-paired) donor serum samples collected in 2000 from the same general population that were analyzed at the time using ion-pair extraction methods with tetrahydroperfluorooctanesulfonate as an internal standard. Eleven of the 100 samples originally collected were reanalyzed with present study methods that involved (13)C- labeled PFOA spiked into the donor samples, original samples, control human plasma, and the calibration curve prior to extraction, and was used as a surrogate to monitor extraction efficiency. Quantification was performed by high performance liquid chromatography tandem mass spectrometry methods. Among the 100 serum samples analyzed for PFOS, the geometric mean was 33.1 ng ml(-1) (95% CI 29.8-36.7) in 2000 compared to 15.1 ng ml(-1) (95% CI 13.3-17.1) in 2005 (p<0.0001) for the 40 donor plasma samples. The geometric mean concentration for PFOA was 4.5 ng ml(-1) (95% CI 4.1-5.0) in 2000 compared to 2.2 ng ml(-1) (95% CI 1.9-2.6) in 2005 (p<0.0001). The decrease was consistent across donors' age and sex. To confirm these preliminary findings, additional sub-sets of year 2000 samples will be analyzed, and a much larger biomonitoring study of other locations is planned. PMID:17267015

  4. Diphtheria toxin IgG levels in military and civilian blood donors in Rio de Janeiro, Brazil

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    F.A.B. Speranza

    2010-01-01

    Full Text Available Serologic data on diseases that are preventable by vaccines are necessary to evaluate the success of immunization programs and to identify susceptible subgroups. In the present study, we determined serum IgG levels against diphtheria toxin of military and civilian blood donors (N = 75; 69.3% males and 30.7% females aged 18-64 years, from the Brazilian Army Biology Institute, Rio de Janeiro, using a commercial diphtheria kit (Diphtheria IgG ELISA; IBL, Germany. Most (63% unprotected military donors were from the older age group of 41 to 64 years. In contrast, the majority (71% of young military donors (18 to 30 years were fully protected. About half of the military donors aged 31 to 40 years were protected against diphtheria. Among the civilians, about 50% of persons aged 18 to 30 years and 31 to 40 years had protective antibody levels against diphtheria as also did 64% of individuals aged 41 to 64 years. All civilians had a similar antibody response (geometric mean = 0.55 IU/mL independent of age group. Military donors aged 18-30 years had higher IgG levels (geometric mean = 0.82 IU/mL than military donors of 41-64 years (geometric mean = 0.51 IU/mL; P > 0.05. In conclusion, the existence of a considerable proportion of susceptible adults supports the position that reliable data on the immune status of the population should be maintained routinely and emphasizes the importance of adequate immunization during adulthood.

  5. The Single Largest Education Donor Comes with Controversy

    Science.gov (United States)

    Finkel, Ed

    2012-01-01

    For the last decade, in districts big and small, the Bill & Melinda Gates Foundation has emerged as the largest private funder of educational efforts. This began with an initiative around small schools in the early to mid-2000s, mostly abandoned now, and has gained traction in the past few years in areas such as teacher evaluation, the Common Core…

  6. Seroepidemiology of HIV, syphilis and hepatitis B and C viruses among blood donors in Bangui, Central African Republic.

    Science.gov (United States)

    Nambei, W S; Rawago-Mandjiza, D; Gbangbangai, E

    2016-05-01

    The aim of this study was to determine the seroprevalence of HIV, the hepatitis B and C viruses, and syphilis as well the risk factors for these diseases among blood donors in Bangui, Central Africa Republic. This cross-sectional study examined samples from donors giving blood in August and September, 2013. HIV1/2 antibodies was screened with the Determine and Unigold HIV tests. Hepatitis B surface antigens were detected by sandwich immunochromatographic methods (DIAspot HBsAg test), and antibodies to HCV by the DIAspot test strip. Syphilis was diagnosed with the VDRL and TPHA methods (Omega Diagnostic, UK). The Chi(2) test was used for statistical analysis. The study included samples from 551 individuals, 350 (63.52%) of whom were frequent volunteer donors. In all, 132 (23.95%) were infected with at least one pathogen. The overall seroprevalence rate was 8.89% for HBV, 4.72% for HCV, 4.36% for syphilis, and 5.98% for HIV. Eight patients had two concomitant infections, with HIV-HBV the most common combination. Compared to long-term volunteers, first-time donors were more often infected by at least one of the pathogens we screened for, most especially HVB (OR = 5.06; 95% CI = 4.22-7.11) and syphilis (OR = 2.05; 95% CI = 2.02-7.44). Our findings indicate the high seroprevalence of transfusion-transmitted infections in blood donated in Bangui. The most common combined infections were HIV-HBV. The most common risk factor was a family history of HBV infection, and especially, mother-child transmission. PMID:27412978

  7. Generation of human induced pluripotent stem cells from a Bombay individual: moving towards "universal-donor" red blood cells.

    Science.gov (United States)

    Seifinejad, Ali; Taei, Adeleh; Totonchi, Mehdi; Vazirinasab, Hamed; Hassani, Seideh Nafiseh; Aghdami, Nasser; Shahbazi, Ebrahim; Yazdi, Reza Salman; Salekdeh, Ghasem Hosseini; Baharvand, Hossein

    2010-01-01

    Bombay phenotype is one of the rare phenotypes in the ABO blood group system that fails to express ABH antigens on red blood cells. Nonsense or missense mutations in fucosyltransfrase1 (FUT1) and fucosyltransfrase2 (FUT2) genes are known to create this phenotype. This blood group is compatible with all other blood groups as a donor, as it does not express the H antigen on the red blood cells. In this study, we describe the establishment of human induced pluripotent stem cells (iPSCs) from the dermal fibroblasts of a Bombay blood-type individual by the ectopic expression of established transcription factors Klf4, Oct4, Sox2, and c-Myc. Sequence analyses of fibroblasts and iPSCs revealed a nonsense mutation 826C to T (276 Gln to Ter) in the FUT1 gene and a missense mutation 739G to A (247 Gly to Ser) in the FUT2 gene in the Bombay phenotype under study. The established iPSCs resemble human embryonic stem cells in morphology, passaging, surface and pluripotency markers, normal karyotype, gene expression, DNA methylation of critical pluripotency genes, and in-vitro differentiation. The directed differentiation of the iPSCs into hematopoietic lineage cells displayed increased expression of the hematopoietic lineage markers such as CD34, CD133, RUNX1, KDR, alpha-globulin, and gamma-globulin. Such specific stem cells provide an unprecedented opportunity to produce a universal blood group donor, in-vitro, thus enabling cellular replacement therapies, once the safety issue is resolved.

  8. HBV vaccination of HCV-infected patients with occult HBV infection and anti-HBc-positive blood donors

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    J.S.F. Pereira

    2006-04-01

    Full Text Available Anti-HBc positivity is a frequent cause of donation rejection at blood banks. Hepatitis B virus (HBV infection may also occur in HBsAg-negative patients, a situation denoted occult infection. Similarly, very low levels of HBV-DNA have also been found in the sera of patients with chronic hepatitis C virus (HCV infection, even in the absence of serum HBsAg. Initially we searched for HBV-DNA in serum of 100 blood donors and 50 HCV-infected patients who were HBsAg negative/anti-HBc positive by nested-PCR and by an HBV monitor commercial test for HBV-DNA. Anti-HBs seroconversion rates were measured in 100 blood donors and in 22 patients with chronic HCV infection after HBV vaccination to determine if the HBV vaccination could eliminate an occult HBV infection in these individuals. Occult HBV infection was detected in proportionally fewer blood donors (6/100 = 6% than chronic hepatitis C patients (12/50 = 24% (P 0.05. All subjects who were HBV-DNA(+ before the first dose of HBV vaccine (D1, became HBV-DNA(- after D1, D2, and D3. Among 22 HCV-positive patients, 10 HBV-DNA(+ and 12 HBV-DNA(-, seroconversion was observed in 9/10 (90% HBV-DNA(+ and in 9/12 (75% HBV-DNA(- subjects (P > 0.05. The disappearance of HBV-DNA in the majority of vaccinated patients suggests that residual HBV can be eliminated in patients with occult infection.

  9. Blood group genotyping: from patient to high-throughput donor screening

    NARCIS (Netherlands)

    B. Veldhuisen; C.E. van der Schoot; M. de Haas

    2009-01-01

    Blood group antigens, present on the cell membrane of red blood cells and platelets, can be defined either serologically or predicted based on the genotypes of genes encoding for blood group antigens. At present, the molecular basis of many antigens of the 30 blood group systems and 17 human platele

  10. Volume-dependent hemodynamic effects of blood collection in canine donors - evaluation of 13% and 15% of total blood volume depletion

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    RUI R.F. FERREIRA

    2015-03-01

    Full Text Available Background: There is no consensus regarding the blood volume that could be safely donated by dogs, ranging from 11 to 25% of its total blood volume (TBV. No previous studies evaluated sedated donors.Aim: To evaluate the hemodynamic effects of blood collection from sedated and non-sedated dogs and to understand if such effects were volume-dependent.Materials and Methods: Fifty three donations of 13% of TBV and 20 donations of 15% TBV were performed in dogs sedated with diazepam and ketamine. Additionally, a total of 30 collections of 13% TBV and 20 collections of 15% TBV were performed in non-sedated dogs. Non-invasive arterial blood pressures and pulse rates were registered before and 15 min after donation. Results: Post-donation pulse rates increased significantly in both sedated groups, with higher differences in the 15% TBV collections. Systolic arterial pressures decreased significantly in these groups, while diastolic pressures increased significantly in 13% TBV donations. Non-sedated groups revealed a slight, but significant, SBP decrease. No clinical signs related to donations were registered.Conclusion: These results suggest that the collection of 15% TBV in sedated donors induces hemodynamic variations that may compromise the harmlessness of the procedure, while it seems to be a safe procedure in non-sedated dogs.

  11. [HTLV-I/II seroprevalence and risk factors associated with infection in a blood donor population in Córdoba, Argentina].

    Science.gov (United States)

    Gallego, S; Maturano, E; Recalde, A; Gastaldello, R; Sileoni, S; Bepre, H; Medeot, S

    2001-01-01

    In this study we have determined the seroprevalence of infections by HTLV-I/II in the blood donor population from the city of Córdoba. A total of 5476 blood donor sera were screened for HTLV-I/II antibodies by particle agglutination assay (PA) (SERODIA HTLV-I, Fujirebio INC, Tokyo, Japan). The reactive sera samples were confirmed by an "in house" indirect immunofluorescence assay (IFA). 14 out of 5476 blood donors studied were PA reactive and were confirmed positive by IFA, showing a prevalence of 0.26% (95% confidence interval: 0.126%-0.394%). All the positive samples, except one, met the criteria for HTLV-I. Although one HTLV-I infected donor was an intravenous drug abuser and two donors were born in highly endemic areas for HTLV-I, no specific risk factors were identified among the others. The demonstration that HTLV-I circulates in blood donor population of Córdoba, points out that the systematic screening of blood for HTLV-I/II antibodies must be implemented in the blood banks, in an attempt to prevent the spread of infections with this oncogenic virus in Argentina.

  12. Prevalence and clinical significance of SEN virus infection in patients with non A-E hepatitis and volunteer blood donors in Shanghai

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    AIM: To explore the prevalence of SEN virus (SENV) in patients with non A-E hepatitis and volunteer blood donors in Shanghai. METHODS: According to the published gone sequences, primers from the conserved region were designed. Then, the prevalence of SEN virus in 30 samples from healthy voluntary blood donors and 30 samples from patients with non A-E hepatitis were detected by nested-PCR of SENV-D/H. Some PCR products were cloned and sequenced. RESULTS: The specificity of genotype-specific PCR was confirmed by sequencing, the SENV DNA was detected in 53.3% of the patients with non A-E hepatitis and 10% of the blood donors. The prevalence of SENV-D/H viremia was significantly higher in patients with non A-E hepatitis than in blood donors (P = 0.0002). SENV-H subtype and SENV-D subtype were found in 2 and 1 samples, respectively from blood donors. SENV-H subtype, SENV D subtype, mixed SENV-D and SENV-H subtype were found in 8, 6 and 2 samples, respectively, from patients with non A-E hepatitis. CONCLUSION: The gone type of SENV in patients with non A-E hepatitis and blood donors in shanghai is D or H subtype, and transfusion is not the only transmitting form of SENV.

  13. Prevalence of antibody to Hepatitis B core antigen and Hepatitis B virus DNA in HBsAg negative healthy blood donors

    OpenAIRE

    Karimi, Gharib; Zadsar, Maryam; Vafaei, Nasrin; SHARIFI, Zohreh; FalahTafti, Mohammad

    2016-01-01

    Background Hepatitis B virus is one of the most important blood born viruses. Although the sensitivity of screening tests has been considerably increased, transmission may still occur due to window period or occult hepatitis B infections (OBIs). This study was aimed at evaluating the prevalence of the anti-HBc and identifying the HBV DNA in HBsAg negative blood donors. Methods The Blood samples from 2031 HBsAg-negative blood donors were divided into three aliquots and tested for anti-HBc, ant...

  14. Human T-Lymphotropic Virus Type 1 and 2 Seroprevalence among first-time blood donors in Chile, 2011-2013.

    Science.gov (United States)

    San Martín, Héctor; Balanda, Monserrat; Vergara, Nicolás; Valenzuela, María Antonieta; Cartier, Luis; Ayala, Salvador; Ramírez, Eugenio

    2016-06-01

    Infection with human T-lymphotropic virus type 1/2 (HTLV-1/2) is a major health problem. HTLV-1/2 infection is endemic in Chile but representative donor prevalence data are lacking. Data on all blood donors in a large network of Chilean blood centers were examined during 2011-2013. Screening of HTLV-1/2 antibodies were measured by enzyme immunoassay (EIA) at all blood banks. Blood samples with anticoagulants from initially reactive blood donors were analyzed by serological confirmation tests (immunofluorescence or recombinant immunoblot) at the HTLV National Reference Laboratory of the Public Health Institute of Chile. Additionally, detection of HTLV-1 and HTLV-2 provirus in peripheral blood mononuclear cells (PBMCs) was performed in all blood donors as confirmatory test. Prevalence rates were calculated. Among 694,016 donors, 706 were seropositive for HTLV-1 (prevalence, 1.02 cases per 1,000; 95% confidence interval [CI], 0.94-1.09), and 97 were seropositive for HTLV-2 (prevalence, 0.14 cases per 1,000; 95%CI, 0.11-0.17). Prevalence of HTLV-1 differed considerably by region, from 0.51 to 1.69 per 1,000. Prevalence of HTLV-2 was similar across the country (0.12-0.16). HTLV-1 prevalence was associated with female sex, older age, and residence in the north of Chile. HTVL-2 prevalence was associated with older age. The HTLV-1 prevalence among Chilean blood donors was relatively high and could be reduced by improving donor recruitment and selection in high prevalence areas. Blood center data may contribute to surveillance for HTLV-1 and HTLV-2 infections. PMID:26538335

  15. Seroprevalence of Human Immunodeficiency Virus, Hepatitis B Virus, Hepatitis C Virus, and Treponema pallidum Infections among Blood Donors on Bioko Island, Equatorial Guinea.

    Directory of Open Access Journals (Sweden)

    Dong-De Xie

    Full Text Available Regular screening of transfusion-transmissible infections (TTIs, such as human immunodeficiency virus (HIV, hepatitis B and hepatitis C virus (HBV and HCV, respectively, and Treponema pallidum, in blood donors is essential to guaranteeing clinical transfusion safety. This study aimed to determine the seroprevalence of four TTIs among blood donors on Bioko Island, Equatorial Guinea (EG.A retrospective survey of blood donors from January 2011 to April 2013 was conducted to assess the presence of HIV, HBV, HCV and T. pallidum. The medical records were analyzed to verify the seroprevalence of these TTIs among blood donations stratified by gender, age and geographical region.Of the total 2937 consecutive blood donors, 1098 (37.39% had a minimum of one TTI and 185 (6.29% harbored co-infections. The general seroprevalence of HIV, HBV, HCV and T. pallidum were 7.83%, 10.01%, 3.71% and 21.51%, respectively. The most frequent TTI co-infections were HBV-T. pallidum 60 (2.04% and HIV-T. pallidum 46 (1.57%. The seroprevalence of HIV, HBV, HCV and T. pallidum were highest among blood donors 38 to 47 years, 18 to 27 years and ≥ 48 years age, respectively (P<0.05. The seroprevalence of TTIs varied according to the population from which the blood was collected on Bioko Island.Our results firstly provide a comprehensive overview of TTIs among blood donors on Bioko Island. Strict screening of blood donors and improved hematological examinations using standard operating procedures are recommended.

  16. Prevalence of Treponema pallidum DNA among blood donors with two different serologic tests profiles for syphilis in São Paulo, Brazil.

    Science.gov (United States)

    Ferreira, S C; de Almeida-Neto, C; Nishiya, A S; Di-Lorenzo-Oliveira, C; Ferreira, J E; Alencar, C S; Levi, J E; Salles, N A; Mendrone-Junior, A; Sabino, E C

    2014-05-01

    The presence of Treponema pallidum DNA was assessed by real-time PCR in samples of blood donors with reactive serologic tests for syphilis. Treponema pallidum DNA was detected in two (1·02%) of 197 samples of VDRL>8, EIA+ and FTA-ABS+ donors, and in no sample from 80 VDRL−, EIA+ and FTA-ABS+ donors. Donors VDRL−, EIA+ and FTA-ABS+ lack demonstrable T. pallidum DNA in their blood and are unlike to transmit syphilis. Donors VDRL>8, EIA+ and FTA-ABS+ carry the risk of syphilis infectivity even in concomitance to antibodies detection. Serologic screening for syphilis may still play a role to prevent its transfusion transmission. PMID:24877236

  17. Era of blood component therapy: time for mandatory pre-donation platelet count for maximizing donor safety and optimizing quality of platelets.

    Science.gov (United States)

    Das, Sudipta Sekhar; Zaman, R U; Biswas, Dipak

    2013-12-01

    Blood bank regulatory agencies including the Drug and Cosmetics Act (DCA) of India do not mandate a predonation platelet count in whole blood donation. Mandating such practice will definitely optimize the quality of random donor platelets (RDP) in terms of platelet yield and patient therapeutic benefit. We observed poor platelet yield in RDP concentrates prepared at our center with a significant number not meeting the DCA guideline of ≥ 4.5 × 10(10) per bag processed from 450 ml of whole blood. Therefore we planned this study to evaluate the pre-donation hematological values in our blood donor population and effect of these values on the quality of platelet concentrates. The prospective study included 221 blood donors eligible for donating 450 ml of whole blood (WB). Following the departmental standard operating procedure (SOP) RDPs were prepared using the 'Top & Bottom' quadruple bag system and automated component extractor. Quality of RDP was assessed as per departmental protocol. All results were recorded and subsequently transcribed to SPSS working sheet. A significant (pplatelets reduced by 0.72 g/dl and 22.1 × 10(6)/ml respectively. Quality of RDPs in terms of platelet yield was significantly better (pplatelet count was >200 × 10(6)/ml. Although platelet yield significantly correlated with the donor platelet count however quality of RDPs in terms of red cell contamination showed no correlation with the donor hematocrit. Platelet yield in random donor platelets is a concern in Eastern India. A platelet yield of 4.5 × 10(10) per bag as mandated by the DCA of India was only achieved when the donor platelet count was >200 × 10(6)/ml. Posttransfusion platelet recovery (PPR) was unsatisfactory in the transfused patient. Introduction of pre-donation platelet count in whole blood donation will maximize donor safety and optimize patient platelet transfusion management.

  18. Health-related quality of life among blood donors with hepatitis B and hepatitis C: longitudinal study before and after diagnosis

    Directory of Open Access Journals (Sweden)

    Francisco Augusto Porto Ferreira

    2015-12-01

    Full Text Available ABSTRACT Introduction: There is evidence that patients suffering from chronic hepatic diseases, including chronic hepatitis B and chronic hepatitis C, have a reduced health-related quality of life. The aim of this study was to evaluate the impact of the notification of test results for hepatitis B and hepatitis C on the quality of life of blood donors. Methods: Over a 29-month period, this study assessed the quality of life of 105 blood donors with positive serological screening tests for hepatitis B and hepatitis C and donors who presented false-positive test results. The Medical Outcome Study 36-Item Short Form Health Survey Questionnaire was applied at three time points: (1 when an additional blood sample was collected for confirmatory tests; (2 when donors were notified about their serological status; and (3 when donors, positive for hepatitis B and hepatitis C, started clinical follow- up. Quality of life scores for the confirmed hepatitis B and hepatitis C groups were compared to the false-positive control group. Results: The domains bodily pain, general health perception, social function, and mental health and the physical component improved significantly in donors with hepatitis C from Time Point 1 to Time Point 3. Health-related quality of life scores of donors diagnosed with hepatitis B and hepatitis C were significantly lower in six and four of the eight domains, respectively, compared to the false-positive control group. Conclusion: A decreased quality of life was detected before and after diagnosis in blood donors with hepatitis B and hepatitis C. Contrary to hepatitis B positive donors, the pos- sibility of medical care may have improved the quality of life among hepatitis C positive donors.

  19. Novel UDP-GalNAc Derivative Structures Provide Insight into the Donor Specificity of Human Blood Group Glycosyltransferase.

    Science.gov (United States)

    Wagner, Gerd K; Pesnot, Thomas; Palcic, Monica M; Jørgensen, Rene

    2015-12-25

    Two closely related glycosyltransferases are responsible for the final step of the biosynthesis of ABO(H) human blood group A and B antigens. The two enzymes differ by only four amino acid residues, which determine whether the enzymes transfer GalNAc from UDP-GalNAc or Gal from UDP-Gal to the H-antigen acceptor. The enzymes belong to the class of GT-A folded enzymes, grouped as GT6 in the CAZy database, and are characterized by a single domain with a metal dependent retaining reaction mechanism. However, the exact role of the four amino acid residues in the specificity of the enzymes is still unresolved. In this study, we report the first structural information of a dual specificity cis-AB blood group glycosyltransferase in complex with a synthetic UDP-GalNAc derivative. Interestingly, the GalNAc moiety adopts an unusual yet catalytically productive conformation in the binding pocket, which is different from the "tucked under" conformation previously observed for the UDP-Gal donor. In addition, we show that this UDP-GalNAc derivative in complex with the H-antigen acceptor provokes the same unusual binding pocket closure as seen for the corresponding UDP-Gal derivative. Despite this, the two derivatives show vastly different kinetic properties. Our results provide a important structural insight into the donor substrate specificity and utilization in blood group biosynthesis, which can very likely be exploited for the development of new glycosyltransferase inhibitors and probes.

  20. Circulating thrombopoietin levels in normal healthy blood donors and in aplastic anemia patients in relation to disease severity

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    Abhay Singh

    2015-01-01

    Full Text Available Background: Thrombopoietin (TPO is the key hematopoietic growth factor regulating the production of platelets from bone marrow megakaryocytes and maintaining platelet hemostasis. This study was done to find any relationship between the levels of thrombopoietin and the severity of disease in patients with aplastic anemia. Materials and Methods: Serum samples were collected from 52 patients with a confirmed diagnosis of aplastic anemia and 45 normal healthy blood donors of both sexes over a period of 2 years, and TPO was estimated by using commercially available TPO-specific-enzyme-linked immunosorbent assay. Results: The median TPO level of 1190 pg/ml (range 625-7651 pg/ml in aplastic anemia patients was significantly higher than the median TPO level of 121.1 pg/ml (81.25-237.7 pg/ml in normal healthy blood donors (P = 0.000. No significant difference was observed in TPO levels of male and female patients (P = 0.453. The median TPO concentrations observed in very severe aplastic anemia, severe aplastic anemia, and nonsevere aplastic anemia were 2765 pg/ml (range 625-6451 pg/ml, 1190 pg/ml (range 672.1-7651 pg/ml, and 1111.5 pg/ml (range 761.1-2289.2 pg/ml, respectively. TPO in patients of very severe aplastic anemia was significantly higher than patients of nonsevere aplastic anemia (P = 0.043, with no significant relation among rest of the groups. Discussion: TPO levels in aplastic anemia patients were significantly higher than in healthy blood donors; however, in aplastic anemia patients TPO levels were significantly higher only in patients with very severe disease.

  1. Seroprevalence of transfusion transmitted infection among blood donors at Jijiga blood bank, Eastern Ethiopia: retrospective 4 years study

    OpenAIRE

    Mohammed, Yusuf; Bekele, Alemayehu

    2016-01-01

    Background A transfusion transmissible infection (TTI) is any infection that is transmissible from person to- person through parenteral administration of blood or blood products. The magnitude of transfusion-transmitted infections (TTI) varies from country to country depending on TTI’s load in that particular population. Measuring their severity, WHO (World Health Organization) has recommended pre-transfusion blood test for Human immunodeficiency virus (HIV), Hepatitis B virus (HBV), Hepatiti...

  2. Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014.

    Science.gov (United States)

    Chiu, Charles Y; Bres, Vanessa; Yu, Guixia; Krysztof, David; Naccache, Samia N; Lee, Deanna; Pfeil, Jacob; Linnen, Jeffrey M; Stramer, Susan L

    2015-08-01

    A newly developed transcription-mediated amplification assay was used to detect chikungunya virus infection in 3 of 557 asymptomatic donors (0.54%) from Puerto Rico during the 2014-2015 Caribbean epidemic. Viral detection was confirmed by using PCR, microarray, and next-generation sequencing. Molecular clock analysis dated the emergence of the Puerto Rico strains to early 2013.

  3. Time trend of the prevalence of hepatitis E antibodies among farmers and blood donors: a potential zoonosis in Denmark

    DEFF Research Database (Denmark)

    Christensen, Peer B; Engle, Ronald E; Hjort, Charlotte;

    2008-01-01

    BACKGROUND: Antibody to hepatitis E virus (anti-HEV) is prevalent in Western countries, where clinical hepatitis E is rarely reported. The aim of this study was to determine the prevalence of anti-HEV among Danish blood donors and Danish farmers. In addition, we compared the prevalence among 2 se......-HEV. CONCLUSION: Anti-HEV was highly prevalent among Danes but has decreased in prevalence over the past 50 years. Our study supports the hypothesis that HEV infection in Denmark may be an asymptomatic zoonotic infection....

  4. Detection of HBsAg and Anti HBc on donors of a blood bank by IRMA and ELISA methods

    International Nuclear Information System (INIS)

    Comparative evaluation of two methods, Immunoradiometric Assay (IRMA) and Enzyme Immunoassay (ELISA), for detecting HBsAg and Anti HBc was made for determining which is the most advantageous and reliable. The study was made on 300 donors of the Hospital San Juan de Dios Blood Bank. In comparison with the reference method (IRMA), ELISA shows 91.67% of sensitivity. The Anti HBc detection by IRMA is more reliable than the HBsAg detection by IRMA and ELISA for determining the carrier state

  5. Donation intensity and metabolic syndrome in active whole-blood donors

    NARCIS (Netherlands)

    Peffer, K.; Verbeek, A.L.M.; Swinkels, D.W.; Geurts-Moespot, A.; Heijer, M. den; Atsma, F.

    2015-01-01

    BACKGROUND AND OBJECTIVES: Increased iron and metabolic syndrome (MetS) go hand in hand. Frequent blood donation depletes iron stores. This study investigates whether high-intensity blood donation is associated with lower MetS prevalence compared with low-intensity blood donation, and whether iron a

  6. Prevalence and trend of hepatitis C virus infection among blood donors in Chinese mainland: a systematic review and meta-analysis

    Directory of Open Access Journals (Sweden)

    Ding Keqin

    2011-04-01

    Full Text Available Abstract Background Blood transfusion is one of the most common transmission pathways of hepatitis C virus (HCV. This paper aims to provide a comprehensive and reliable tabulation of available data on the epidemiological characteristics and risk factors for HCV infection among blood donors in Chinese mainland, so as to help make prevention strategies and guide further research. Methods A systematic review was constructed based on the computerized literature database. Infection rates and 95% confidence intervals (95% CI were calculated using the approximate normal distribution model. Odds ratios and 95% CI were calculated by fixed or random effects models. Data manipulation and statistical analyses were performed using STATA 10.0 and ArcGIS 9.3 was used for map construction. Results Two hundred and sixty-five studies met our inclusion criteria. The pooled prevalence of HCV infection among blood donors in Chinese mainland was 8.68% (95% CI: 8.01%-9.39%, and the epidemic was severer in North and Central China, especially in Henan and Hebei. While a significant lower rate was found in Yunnan. Notably, before 1998 the pooled prevalence of HCV infection was 12.87% (95%CI: 11.25%-14.56% among blood donors, but decreased to 1.71% (95%CI: 1.43%-1.99% after 1998. No significant difference was found in HCV infection rates between male and female blood donors, or among different blood type donors. The prevalence of HCV infection was found to increase with age. During 1994-1995, the prevalence rate reached the highest with a percentage of 15.78% (95%CI: 12.21%-19.75%, and showed a decreasing trend in the following years. A significant difference was found among groups with different blood donation types, Plasma donors had a relatively higher prevalence than whole blood donors of HCV infection (33.95% vs 7.9%. Conclusions The prevalence of HCV infection has rapidly decreased since 1998 and kept a low level in recent years, but some provinces showed relatively

  7. The Population Structural Characteristics of Rh (-) Blood Donor from 2005 to 2015 in Qujing City analysis of Blood Transfusion and Storage Strategies for Clinical Emergency%曲靖市2005-2015年Rh(-)献血者人群结构特征急救用血及保留策略分析

    Institute of Scientific and Technical Information of China (English)

    刘琼芬; 余桂华; 黄会武; 杨凤碧; 吴伟华; 荀春志

    2016-01-01

    目的:了解曲靖市近11年来Rh(-)献血者人群结构状况、为改进和完善献血者宣传招募提供依据,提高固定献血者的比例,采集充足血液。方法通过对2005—2015年参加无偿献血的Rh(-)献血者资料性别、年龄结构、职业状况、民族构成、文化程度、献血类型、血型分布运用统计方法进行比较分析。结果11年共有644人Rh(-)献血者参加无偿献血,男性献血者明显高于女性,男女比例为1.78:1,文化程度以初级中等教育、大学本科及大学专科为主占(65.53%),职业分布以农民为主占(54.50%);年龄以25~34及35~45岁所占比例较大(占72.83%),644人Rh(-)献血者中,固定献血23人仅占3.57%;流失399人占61.95%。临床用血从2005年的76.3 U增加到2015年的306.3 U,血液库存时有季节性偏型存在。结论Rh(-)献血者人群在性别、职业、文化程度和血型方面均有显著特征,在制定献血者招募策略时,应依据不同的目标人群制定不同的宣传招募方案,稳定、巩固及壮大Rh(-)献血者队伍,积极招募动员更多的献血者,采集充足的血液,保障Rh(-)患者得到及时救治。%Objective This paper aims to gather information of the population structural characteristics of Rh (-) blood donor city for the past 11 years, provides publicity and recruitment for the improvement of blood donors for blood donors and increases the fixed proportion of adequate blood collection. Methods Based on the gender, age structure, occupation status, ethnic composition, education level, blood type of the Rh (-) blood donors from 2005 to 2015, the paper analyzes the blood type distribution by using statistical methods. Results For the past 11 years, a total of 644 Rh (-) blood donors took part in the voluntary blood donation, and the male donors was significantly higher than that in female with the ratio of male to female of 1.78:1. For the education level part, donors are mainly with

  8. Impact of Early Blood Transfusion After Kidney Transplantation on the Incidence of Donor-Specific Anti-HLA Antibodies.

    Science.gov (United States)

    Ferrandiz, I; Congy-Jolivet, N; Del Bello, A; Debiol, B; Trébern-Launay, K; Esposito, L; Milongo, D; Dörr, G; Rostaing, L; Kamar, N

    2016-09-01

    Little is known about the impact of posttransplant blood transfusion on the sensitization of anti-HLA antibodies and the formation of donor-specific antibodies (DSAs). The aims of our study were to determine the 1-year incidence of DSAs (assessed using a solid-phase assay) and antibody-mediated rejection (AMR) in kidney transplant patients who had or had not received a blood transfusion during the first year after transplantation. Included were 390 non-HLA-sensitized patients who had received an ABO-compatible kidney transplant and had not previously or simultaneously received a nonkidney transplant. Overall, 64% of patients received a red blood cell transfusion within the first year after transplantation, most within the first month. The overall 1-year incidence of DSAs was significantly higher in patients that had undergone transfusion (7.2% vs. 0.7% in patients with no transfusion, p transfusion group (n = 15, 6%) compared with the nontransfusion group (n = 2, 1.4%; p = 0.04). Blood transfusion was an independent predictive factor for de novo DSA formation but not for AMR. Patients who had a transfusion and developed DSAs were more often treated with cyclosporin A (n = 10, 55.5%) rather than tacrolimus (n = 45, 19.4%; p = 0.0001). In conclusion, early posttransplant blood transfusion may increase immunological risk, especially in underimmunosuppressed patients.

  9. Stress and coping in HIV-positive former plasma/blood donors in China: a test of cognitive appraisal theory.

    Science.gov (United States)

    Meade, Christina S; Wang, Jianping; Lin, Xiuyun; Wu, Hao; Poppen, Paul J

    2010-04-01

    Throughout the 1990s, many villagers in rural China were infected with HIV through commercial plasma/blood donation. These former plasma/blood donors (FPDs) experienced many HIV-related stressors. This study tested a cognitive appraisal model of stress and coping in a sample of HIV-positive adult FPDs. Participants (N = 207) from multiple villages completed a battery of questionnaires assessing HIV-related stress, HIV symptoms, cognitive appraisal, coping behaviors, and psychological distress. Participants reported high levels of HIV-related stress, depression, and anxiety. In a structural equation model, greater HIV-related stress, HIV symptoms, and threat appraisal were directly associated with psychological distress. HIV-related stress was also indirectly associated with psychological distress through threat appraisal. In a second model, coping was found to mediate the relationship between challenge appraisal and psychological distress. Results support the utility of cognitive appraisal theory. Stress management interventions targeting HIV-positive FPDs in China are indicated.

  10. Strongyloidiasis in Po valley: serological and parasitological screening in blood donors of Cremona

    Directory of Open Access Journals (Sweden)

    Paolo Mangoni

    2012-09-01

    Full Text Available Backgroud. Strongyloides stercoralis is a ubiquitous nematode that is present also in Italy in a hypoendemic form, especially in the Po Valley. It is responsable for strongyloidiasis, an intestinal parasitosis usually asymptomatic or paucy-symptomatic that occasionally can cause a hyperinfestation syndrome and disseminated disease. Objectives. To determine the presence of S. stercoralis in asympomatic people afferent to the Cremona hospital by serological screening and direct microscopic search. Study Design. In the period from December 1, 2010 to June 30, 2011 we screened 154 donors with absolute eosinophilia >500/μL and percentage of eosinophils 9%. The research of anti-S. stercoralis antibodies was performed through an ELISA method (S.ratti Bordier Products. Donors positive to the screening test were tested for direct parasite search through with phormol-ethyl acetate stool concentration (FEA and culture on S. stercoralis Agar (Biolife, Milan. Results. 13 donors were positive to the screening test (8.4%. 10 of these 13 donors performed coproparassitologic examination. S. stercoralis was identified in the stools of 3/10 tested patients through culture and in 2/10 through FEA. Conclusions. The study results are in line with the data reported in the literature for similar geographic areas. Among the risk factors are to be counted the type of job (agriculture, and the domicile in the rural environment. The culture in S. stercoralis Agar has proved much more sensitive than FEA. The serological diagnosis represent a valuable contribution to traditional coproparassitologic examination, particularlywhen screening asymptomatic individuals at risk.

  11. Return behavior of occasional and multigallon blood donors: the role of theory of planned behavior, self-identity, and organizational variables

    NARCIS (Netherlands)

    Wevers, A.; Wigboldus, D.H.J.; Baaren, R.B. van; Veldhuizen, I.J.T.

    2014-01-01

    BACKGROUND: For blood establishments it is important that blood donors return for a donation. Past research has stressed the importance of theory of planned behavior (TPB) on return behavior, but self-identity (SI) and organizational variables (OVs) might play a role as well. This study added SI and

  12. [Level of prolactin, gonadotrophin, testosterone, and alpha fetoprotein in serum of blood donors with antibodies to hepatitis c virus (anti-HCV)].

    Science.gov (United States)

    Rzeszowska, G; Jagiełło-Wójtowicz, E; Krawczuk, G; Baran, E; Bielec, D; Fijałka-Rymar, M; Surmaczyńska, B

    1993-01-01

    The study was aimed at the determination of serum levels of the investigated hormones (prolactin, gonadotrophins, testosterone and alpha-fetoprotein) in blood donors with antibodies to hepatitis C virus. The observed group consisted of 50 men of age between 22 and 47 years. The blood serum concentrations of hormones were determined by RIA method using the ready made reagent kits. No characteristic changes were observed in the serum hormones levels except for prolactin. Mean value of serum prolactin concentration was higher in blood donors than those in the control group. PMID:7688903

  13. Prevalence of and risk factors for HIV infection in blood donors and various population subgroups in Ethiopia.

    Science.gov (United States)

    Sentjens, R E J H; Sisay, Y; Vrielink, H; Kebede, D; Adèr, H J; Leckie, G; Reesink, H W

    2002-04-01

    The aim was to determine the prevalence of HIV infection and risk factors for HIV infection in various population subgroups in Ethiopia. Serum panels from blood donors (n = 2610), from various population subgroups in Ethiopia were tested for anti-HIV-1/2 by ELISA. All ELISA repeatedly reactive samples were subjected for confirmation by immunoblot (IB) and anti-HIV-1 and anti-HIV-2 specific ELISAs. 155/2610 (5.9%) blood donors were HIV-1 infected. Of pregnant women, 84/797 (10.5%) were HIV-1 infected, and 1/797 (0.1%) was HIV-2 infected. 1/240 (0.4%) individuals from the rural population were HIV-1 infected. 198/480 (41.3%) female attendees, and 106/419 (25.3%) male attendees at sexual transmitted disease (STD) clinics were HIV-1 infected. One (0.2%) male, and 2 (0.4%) female STD patients were infected with both HIV-1 and HIV-2. It was concluded that the prevalence of HIV-1 infection varied from 0.4% among urban residents to 25.3-41.3% among STD attendees. There is a low prevalence of HIV-2 present in Ethiopian subjects. Risky sexual behaviour is significantly associated with HIV-infection in Ethiopia. PMID:12002540

  14. [Reducing the need in donor blood in reconstructive surgery of the aorta: using a Cell Saver apparatus].

    Science.gov (United States)

    Mierbekov, E M; Ilialetdinov, I D

    2004-01-01

    The possibility of using a Cell Saver "Sequestra 1000" apparatus (Medtronics) for reducing the need for donor blood in reconstructive surgery for aortic aneurism was evaluated within the case study. Thirty-seven patients, who were made different reconstructive procedures on the ascending and abdominal aorta, were examined. Twelve patients with aneurism in the ascending aorta section (AAS) and with insufficiency of the aortal valve were made prosthesis of AAS and aortal valve under extracorporeal circulation (AEC). Twenty-five patients, who were operated on the abdominal aorta section, were resected aneurysm with linear prosthesis (9), aorto-hip bifurcational alloprosthesis (15) and branching of arterio-venous fistula (1). Five patients with aneurysm in the abdominal aorta, including 3 cases of aneurysm rupture, were operated on the emergency basis. The use of a Sequestra 1000 (Medtronics) apparatus based on the Cell Saver technology aorta reconstructions cut essentially the need in donor packed red blood cells at the intraoperative (3.6 times) and postoperative (2.8 times) stages. PMID:15573718

  15. Dengue viremia in blood donors in Northern India: Challenges of emerging dengue outbreaks to blood transfusion safety

    OpenAIRE

    Sadhana Mangwana

    2015-01-01

    Backdround: Emerging infectious diseases pose threats to the general human population; including recipients of blood transfusions. Dengue is spreading rapidly to new areas and with increasing frequency of major outbreaks. Screening blood for dengue antigens in dengue-endemic countries would be costly and should, therefore, be recommended only after careful assessment of risk for infection and cost. Aim: A prospective study was conducted to establish the magnitude of the threat that dengue pos...

  16. HBV, HCV and HIV seroprevalence among blood donors in Istanbul, Turkey: how effective are the changes in the national blood transfusion policies?

    Directory of Open Access Journals (Sweden)

    Ali Acar

    2010-02-01

    Full Text Available The national blood transfusion policies have been changed significantly in recent years in Turkey. The purpose of this study was to determine the prevalence of HBV, HCV, and HIV in blood donors at the Red Crescent Center in Istanbul and to evaluate the effect of changes in the national blood transfusion policies on the prevalence of these infections. The screening results of 72695 blood donations at the Red Crescent Center in Istanbul between January and December 2007 were evaluated retrospectively. HBsAg, anti-HCV, and anti-HIV-1/2 were screened by microparticle enzyme immunoassay (MEIA method. Samples found to be positive for anti-HIV 1/2 and anti-HCV were confirmed by Inno-Lia HCV Ab III and Inno-Lia HIV I/II Score, respectively. The seropositivity rates for HBsAg, anti-HCV, and anti-HIV-1/2 were determined as 1.76%, 0.07%, and 0.008%, respectively. Compared to the previously published data from Red Crescent Centers in Turkey, it was found that HBV and HCV seroprevalances decreased and HIV seroprevalance increased in recent years. In conclusion, we believe that the drop in HBV and HCV prevalence rates are likely multifactorial and may have resulted from more diligent donor questioning upon screening, a higher level of public awareness on viral hepatitis as well as the expansion of HBV vaccination coverage in Turkey. Another factor to contribute to the decreased prevalence of HCV stems from the use of more sensitive confirmation testing on all reactive results, thereby eliminating a fair amount of false positive cases. Despite similar transmission routes, the increase in HIV prevalence in contrast to HBV and HCV may be linked to the increase in AIDS cases in Turkey in recent years.

  17. 滨州市无偿献血者人类免疫缺陷病毒感染情况调查分析%Investigation and analysis of human immunodeficiency virus infection status of unpaid blood donors in Binzhou City

    Institute of Scientific and Technical Information of China (English)

    李洪波; 王静; 孙春莲

    2015-01-01

    Objective To analyze the human immunodeficiency virus (HIV) infection status of unpaid blood donors in Binzhou City Shandong Province,and to discuss the development trend of HIV infection rate of Binzhou City's unpaid donors,and thus putting forward corresponding countermeasures.Methods From January 2004 to December 2013,a total of 346 424 unpaid blood donors who donated blood in Binzhou Blood Center were chosen as research objects.Among them,there were 226 215 cases of men blood donors and 120 209 cases of women blood donors.The age was ranged from 18 to 55 years old,and the average age was 32.5 years old.All blood donors met the requirement of the Health Inspection Requirements of Blood Donors.Informed consent was obtained from all blood donors before blood collection,and they all signed in the column of informed consent.In order to guarantee the authenticity of the inspection findings,enzymelinked immunosorbent assay (ELISA) tests were conducted to test blood samples twice.The results were tested and verified again by testing blood in the same test tube and blood in the blood bag if there was one reagent positive reaction in the first test.It was positive if one of two holes showed positive reaction in two-hole experiment,and then the blood samples were sent to Shandong Center for Disease Control and Prevention for HIV confirmation experiment.HIV positive incidence,patient occupation,educational level,age and gender ratio were analyzed by statistical methods.Results The infection rate of HIV in unpaid blood donors in Binzhou from 2004 to 2013 was 6.35/100 000 which was in low infective trend,but it was in a rising trend year by year.Among all the umpaid blood donors,the infection rate of HIV in peasant and unemployed were much higher than those of other occupations,the lower education level,the higher infection rate of HIV,the infection rate of HIV in unpaid blood donors of ≤ 35 years old was higher than those of > 35 years old,and the infection rate of HIV

  18. 无偿献血者的心理护理体会%Psychological Nursing Experience of Voluntary Blood Donors

    Institute of Scientific and Technical Information of China (English)

    曹阳

    2015-01-01

    目的:探究对无偿献血者应采取的心理护理措施和效果。方法选取2013年9月~2015年1月38例无偿献血人员进行护理研究,随机分组,实验组23例人员给予综合的心理护理,对照组15例人员不采取心理护理措施,观察护理效果和不良反应的发生情况。结果实验组没有患者出现不良反应,对照组不良反应的发生率为13.3%,差异有统计学意义(P<0.05)。结论无偿献血者在献血中护理人员给予心理护理,能够缓解心理障碍和负面情绪,使其在良好状态下,愉快、安全的献血,减少不良反应的发生率。%Objective To study the Psychological nursing method and its effect for voluntary blood donors.Methods Chose 38 voluntary blood donors who were treated in hospital from September 2013 to January 2015 and separated them into two groups at random,32 patients in study group were given comprehensive psychological nursing and 15 patients in control group were given non-psychological nursing,then compared patients’ nursing effects and side-effect incidence between two groups. Results There was no side-effect to be seen in study group,while side-effect incidence in control group was 13.3%,there was a treatment differential between two groups and such a differential had statistic value(P< 0.05).Conclusion Psychological nursing is of efficacy for voluntary blood donors,it is conducive to aleviating donors’ psychological barriers and negative mood in order to have them donate blood under favorable condition and reduce side-effect incidence.

  19. Human T-Lymphotropic Virus Type I (HTLV-1) Infection among Iranian Blood Donors: First Case-Control Study on the Risk Factors.

    Science.gov (United States)

    Hedayati-Moghaddam, Mohammad Reza; Tehranian, Farahnaz; Bayati, Maryam

    2015-11-04

    Human T-cell lymphotropic virus type 1 (HTLV-1) infection is an endemic condition in Northeast Iran and, as such, identification of risk factors associated with the infection in this region seems to be a necessity. All the possible risk factors for HTLV-1 seropositivity among first-time blood donors were evaluated in Mashhad, Iran, during the period of 2011-2012. Blood donation volunteers were interviewed for demographic data, medical history, and behavioral characteristics and the frequencies of risk factors were compared between HTLV-1 positive (case) and HTLV-1 negative (control) donors. The data was analyzed using Chi square and t-tests. Logistic regression analysis was performed to identify independent risk factors for the infection. Assessments were carried out on 246 cases aged 17-60 and 776 controls aged 17-59, who were matched based on their ages, gender, and date and center of donation. Logistic analysis showed low income (OR = 1.53, p = 0.035), low educational level (OR = 1.64, p = 0.049), being born in the cities of either Mashhad (OR = 2.47, p = 0.001) or Neyshabour (OR = 4.30, p risk factors for HTLV-1 infection, such as prolonged breastfeeding and sexual promiscuity. Pre-donation screening of possible risk factors for transfusion-transmissible infections should also be considered as an important issue, however, a revision of the screening criteria such as a history of transfusion for more than one year prior to donation is strongly recommended.

  20. Outcomes of peripheral blood stem cell transplantation in patients from human leukocyte antigen matched or mismatched unrelated donors

    Institute of Scientific and Technical Information of China (English)

    Cao Tingting; Li Yanfen; Wang Quanshun; Li Honghua; Bo Jian; Zhao Yu; Jing Yu

    2014-01-01

    Background Allogeneic peripheral blood stem cell transplantation from unrelated donors (UR-PBSCT) is an alternative treatment for many hematologic diseases due to lack of human leukocyte antigen (HLA)-identical sibling donors.This study aimed to evaluate the impact of the degree of the HLA match on the clinical efficacy of UR-PBSCT.Methods Patients who underwent UR-PBSCT from September 2003 to September 2012 were retrospectively investigated.They were divided into three groups according to high-resolution molecular typing.SPSS version 17.0 was used to analysis and compare the statistics of engraftment,incidence of GVHD,other complications and survival among the groups.Results One hundred and eleven patients received UR-PBSCT,60 of them with an HLA matched donor (10/10),36 of them with a one locus mismatched donor (9/10),and 15 of them with a two loci mismatched donor (8/10).Similar basic characteristics were found in the three groups.No differences were found in engraftment of myeloid cells or platelets in the three groups (P>0.05).Two-year cumulative incidence of relapse,overall survival (OS) and disease-free survival (DFS) among those three groups were similar (P>0.05).The cumulative incidence of 100-day Ⅲ-Ⅳ aGVHD in the HLA matched group and the one HLA locus mismatched group were significantly lower than that in the two HLA loci mismatched group (3.3%,8.6%,and 26.7%,P=0.009).The occurrence rate of new pulmonary infections in the HLA matched group was lower than in the two HLA mismatched groups (26.67%,52.78%,and 41.18%,P=0.035).The cumulative incidence of 100-day and 2-year transplantation related mortality (TRM) in two HLA loci mismatched group was higher than in the HLA matched group and in the one HLA locus mismatched group,(8.4%,11.8% and 33.3%,P=0.016) and (12.3%,18.7% and 47.5%,P=0.002).Conclusions HLA mismatch will not significantly impact the engraftment or 2-year survival after UR-PBSCT,but two mismatched HLA loci may

  1. A comprehensive serological and supplemental evaluation of hepatitis B “seroyield” blood donors: A cross-sectional study from a tertiary healthcare center in India

    OpenAIRE

    Prashant Pandey; Aseem K Tiwari; Dara, Ravi C.; Geet Aggarwal; Ganesh Rawat; Vimarsh Raina

    2015-01-01

    Background: The present study addressed the interesting findings of supplemental evaluation of hepatitis B "seroyield" donors. Materials and Methods: Each blood donor sample was tested for anti-human immunodeficiency virus type I (HIV-I)/HIV type II (HIV-II), HBsAg, and anti-hepatitis C virus (HCV) antibody by enhanced chemiluminescence method and subjected to individual donor-nucleic acid testing (NAT) for HIV-I, hepatitis B virus (HBV), and HCV. NAT test was performed using the eSAS system,...

  2. Presence of medication taken by blood donors in plasma for transfusion

    NARCIS (Netherlands)

    Van Tilborgh, A.J.W.; Touw, D.J.; Wiersum-Osselton, J.C.; Zijlker-Jansen, P.Y.; Hudig, F.; Schipperus, M.R.

    2013-01-01

    Background: The TRIP national hemovigilance and biovigilance office receives reports on side effects and incidents associated with the transfusion of labile blood products. The findings are publicly reported in annual hemovigilance reports. The category of anaphylactic reaction, defined as allergic

  3. Hepatitis G virus exposure in dialysis patients and blood donors in Isfahan-Iran

    Directory of Open Access Journals (Sweden)

    Maryam Salehi

    2014-01-01

    Conclusions: HD patients are at increased risk of HGV infection in Isfahan-Iran. Since hepatitis G is a good predictor for parenteral transmission, it is suggested to test all of the blood for transfusion for HGV infection.

  4. Seroprevalencia de VHB, VHC y VIH en donadores de sangre en Irapuato, México Seroprevalence of HBV, HCV and HIV in blood donors in Irapuato, Mexico

    Directory of Open Access Journals (Sweden)

    Miguel Angel Carreto-Vélez

    2003-01-01

    General Hospital No. 2 Family Medicine Unit, of the Mexican Social Security Institute in Irapuato, Mexico. MATERIAL AND METHODS: A cross-sectional descriptive study. Data was recorded on blood bank forms, and risk factors and illnesses were studied in the 7,056 blood donors at the General Hospital No. 2 Family Medicine Unit, of the Mexican Social Security Institute in Irapuato, Guanajuato, Mexico, over a period of two years (from July 1998 to June 2000. A sample of 4,010 donors was obtained, each of whom underwent serological tests for HBV, HCV and HIV, serotypes 1 and 2, using an enzymatic immunoassay of third generation in serum or human plasma; seroprevalence rate of seropositive donors was calculated and stratified by age and sex. RESULTS: The combined seroprevalence for HBV, HCV and HIV was 2.5% (101; HCV was 1.14% (46, HBV, 1.12% (45, and HIV, 0.24% (10. In males, HBV was 1.04% (33, HCV 1.07% (34, and HIV, 0.28% (9. In females, HBV was 1.42% (12, HCV was 1.42% (12, and HIV was 0.11% (1. Seropositive males had a 2.4 higher rate as compared to females. CONCLUSIONS: The seroprevalence of viral markers was greater than that reported in previous studies carried out in Mexico, which suggests that sexual transmission was the principal mechanism of infection; this reflects poor health education and the need to carefully select potential donors.

  5. Hepatitis B virus screening in contacts of blood donors with antibodies against core protein (anti-HBc, but without surface antigen (HBsAg

    Directory of Open Access Journals (Sweden)

    Hildenete Monteiro Fortes

    2006-03-01

    Full Text Available To increase blood safety Brazil introduced screening for anti-HBc among blood donors in 1993. There was a decrease in the hepatitis B virus (HBV transmission, but this measure identified a great number of HBsAg-negative, anti-HBc-positive donors. Surveillance policy determines that contacts of HBV carriers should be screened to HBV markers, but there is no recommendation about how to guide contacts of HBsAg-negative, anti-HBc-positive donors. Aiming to evaluate whether the contacts of this group are at greater risk for HBV infection, a cross-sectional study was performed to compare prevalence of HBV infection between contacts of HBsAg-positive blood donors (group I and contacts of HBsAg-negative, anti-HBc-positive donors (group II. Contacts were submitted to a questionnaire and blood tests for HBV markers. In group I (n = 143, 53 (37.1% were anti-HBc-positive and 11 (7.7% were HBsAg-positive. In group II (n = 111, there were 9 and 0.9%, respectively. HBV exposure was associated with group I, sexual activity, blood transfusion, being one of the donor's parents, and living for more than ten years with the donor. Regarding the families as sample units, it was more common to find at least one member with HBV markers (p < 0.05 among the families of group I compared to group II. Contacts of HBsAg-negative, anti-HBc-positive individuals presented a much lower risk of having already been exposed to HBV and there is no need to screen them for HBV in low to moderate prevalence populations.

  6. An analysis of HIV infections among blood donors from 2005-2011 in Qujing%曲靖市2005-2011年献血者HIV感染情况分析

    Institute of Scientific and Technical Information of China (English)

    刘琼芬; 荀春志; 余桂华; 高建萍

    2012-01-01

    Objective To explore HIV infections among blood donors from 2005 - 2011 in Qujing, to probe the efficient strategy of recruiting blood donors and to put an end to hidden blood risks. Methods Retrospective analysis was conducted by using statistical software SPSS 13. 0 among HIV infected blood donors from 2005 - 2011. Results A total of 119 HIV infected persons were identified from 153 405 blood donors. The infection rate was 0. 08%, and among the infected persons the youth aged 18 - 29 years accounted for 51. 8% (x2 =23. 60 P<0. 01) , which was higher in males than in females(x2 =6. 22,P = 0. 01). In view of occupation, most were migrant workers and factory workers, the infected rate among them was 30. 25% and 15. 12% , respectively. As to the education level, the people having middle schooling or below accounted for 48. 73%. Conclusion The blood donors infected with HIV in Qujing were mainly young migrant workers who were sexually active and with lower educational background.%目的 为了解曲靖市献血者感染艾滋病病毒(HIV)情况,探讨有效的献血者征询策略,杜绝血液安全隐患.方法 对2005 -2011年献血者HIV感染情况进行回顾性分析,数据统计分析采用SPSS 13.0统计软件进行.结果 153405人次献血者中,共检出HIV感染者119人,检测人群感染率为0.08%.HIV感染者中,18~29岁的青年占51.8%(x2=23.60,P<0.01),男性高于女性(x2=6.22,P=0.01),农民工和工人分别占感染总数的30.25%、15.12%,初中及以下占感染总数的48.73%.结论 曲靖市献血者HIV感染主要是以处于性活跃期、文化素质较低的青壮年农民工为主.

  7. Prevalence of active HCV infection among the blood donors of Khyber Pakhtunkwa and FATA region of Pakistan and evaluation of the screening tests for anti-HCV

    Directory of Open Access Journals (Sweden)

    Khan Sanaullah

    2011-04-01

    Full Text Available Abstract Hepatitis C is a fatal liver disease caused by the hepatitis C virus. In this study, blood donors, from various districts of the KPK province and the federally administered tribal area (FATA of Pakistan were tested for anti-HCV antibodies and HCV RNA by ICT (Immuno-chromatographic test, ELISA and RT-PCR. Out of the 7148 blood donors, 224 (3.13% were positive for anti-HCV antibodies by ICT, 135 (1.89% by ELISA while 118 (1.65% blood donors had active HCV infection as detected by RT-PCR. We suggest that ELISA should be used for anti-HCV screening in public sector hospitals and health care units.

  8. Motivational Differences between Whole Blood and Apheresis Donors in Quebec, Canada: A Questionnaire-Based Survey in a Voluntary Nonremunerated Context

    Directory of Open Access Journals (Sweden)

    Johanne Charbonneau

    2015-01-01

    Full Text Available Background. Finding ways to recruit apheresis donors is crucial. The aim of this study was to provide a quantitative analysis of the motivations of regular plasma/platelets donors (PPDs in comparison with those of regular whole blood donors (WBDs, in a voluntary and nonremunerated context. Study Design and Methods. Motives to donate blood and demographic characteristics were collected through questionnaires completed by 795 WBDs and 473 PPDs. Chi-square tests were completed to determine which motivations stand out across the two blood donor groups. Results. The motivator selected by the highest percentage was “my blood can save lives.” Comparison of WBDs and PPDs showed that 12 out of 23 items were statistically significantly different from one group to another. Conclusion. The belief that helping others is in their nature is more prevalent among PPDs. In this sense, their profile is unique. Four other motivators distinguish this group from the WBDs: “I think there is a strong need for blood products,” “it gives me a sense of pride,” “I like to have goals,” and “I receive telephone reminders.” These motivators point to the role the ongoing support provided by blood collection agencies (BCAs plays with PPDs.

  9. Impact of a confirmatory RhD test on the correct serologic typing of blood donors

    Directory of Open Access Journals (Sweden)

    Luciana Cayres Schmidt

    2015-10-01

    Full Text Available BACKGROUND: The RHD gene is highly polymorphic, which results in a large number of RhD variant phenotypes. Discrepancies in RhD typing are still a problem in blood banks and increase the risk of alloimmunization. In this study, the RhD typing strategy at a blood bank in Brazil was evaluated.METHODS: One-hundred and fifty-two samples typed as RhD negative and C or E positive by routine tests (automated system and indirect antiglobulin test using the tube technique were reevaluated for RhD status by three methods. The method with the best performance was implemented and evaluated for a period of one year (n = 4897 samples. Samples that were D positive exclusively in the confirmatory test were submitted to molecular analysis.RESULTS: The gel test for indirect antiglobulin testing with anti-D immunoglobulin G (clone ESD1 presented the best results. Seventy samples (1.43% previously typed as RhD negative showed reactivity in the gel test for indirect antiglobulin testing and were reclassified as D positive. D variants that may cause alloimmunization, such as weak D type 2 and partial DVI, were detected.CONCLUSION: The confirmatory RhD test using the gel test for indirect antiglobulin testing represents a breakthrough in transfusion safety in this blood center. Our results emphasize the importance of assessing the blood group typing strategy in blood banks.

  10. Donor-assisted Ethno-Nationalism and Health Education Policy in Bhutan

    DEFF Research Database (Denmark)

    Schmidt, Johannes Dragsbæk

    and objectives. This will be exemplified with reference to an analysis of two key policies education and health which should be understood in their own right as state-sanctioned but donor financed policies inserted into the context with elite and government promotion of Gross National Happiness (GNH......), Bhutanization, decentralization and guided democracy, The paper is divided into four sections. The first section gives an overview of what can be defined as ‘dependency through self-reliance and selection’. The government and developmentalist elite and bureaucracy seems to be able to choose consciously who...

  11. Evaluation of the Procleix Ultrio Plus ID NAT assay for detection of HIV 1, HBV and HCV in blood donors

    Directory of Open Access Journals (Sweden)

    Raj Nath Makroo

    2015-01-01

    Full Text Available Introduction: The Procleix Ultrio Plusassay is a new-generation qualitative in vitro nucleic acid amplification test used to screen for human immunodeficiency virus type 1 (HIV-1 RNA, hepatitis C virus (HCV RNA and hepatitis B virus (HBV DNA in blood donors. This study was performed to compare the Procleix Ultrio assay with the new-generation Procleix Ultrio Plus Nucleic Acid Test (NAT assays. Materials and Methods: Ten thousand three hundred and two donor samples were run in parallel for ID NAT using the Procleix Ultrio and the Procleix Ultrio Plus assay. Simultaneously, enzyme-linked immunosorbent assay testing was performed on an EVOLIS Walk away System for HIV, HCV, HBsAg and anti-HBc. Reactive samples were confirmed using polymerase chain reaction. Results: In the 10,302 samples tested during the study period, we identified 15 NAT yields, and all these revealed HBV DNA in the discriminatory assays. Eight of these were exclusive yields from the Ultrio Plus assay and the remaining seven cases were determined as HBV NAT yield, both by the Procleix Ultrio as well as the Ultrio Plus assays, i.e. "Combined" yields. No HCV or HIV 1 yields were detected during the study period by either of two assays. Conclusion: With an overall yield rate of 1 in 687 and an exclusive yield rate of 1 in 1287, the Procleix Ultrio Plus assay proved to be highly sensitive in detecting occult HBV infections.

  12. Rapid and simple hepatitis assays: encouraging results from a blood donor population in Zimbabwe.

    OpenAIRE

    Mvere, D.; Constantine, N. T.; Katsawde, E.; Tobaiwa, O; Dambire, S.; Corcoran, P.

    1996-01-01

    A rapid assay to detect antibodies to hepatitis C virus (HCV) in serum and two rapid/simple assays to detect hepatitis B surface antigen (HBsAg) in whole blood/serum were evaluated for their accuracy and suitability at the National Blood Transfusion Service, Harare, Zimbabwe. For this purpose, a total of 206 sera (196 routinely collected and 10 frozen) were tested using the HCV-SPOT (Genelabs Diagnostics), the SimpliRED HBsAg test (AGEN), and the Dipstick-HBsAg (PATH/Immuno-Chemical Laborator...

  13. Standardization of B19 IgG ELISA to study the seroepidemiology of parvovirus B19 in North Indian voluntary blood donors

    OpenAIRE

    Kishore Janak; Srivastava Manisha; Choudhary Nabjyoti

    2010-01-01

    Backgound and Objectives: Parvovirus B19 (B19) being a non-enveloped DNA virus is hence thermo-stable to the current methods of viral inactivation. Therefore transfusion of blood or its component from a viremic donor to non-immune recipients may result in transfusion-transmitted B19 infection with occasional sinister complications. The serologically naοve blood donor population in our country has not been studied. Hence a study was designed to find the sero-status of B19 virus in normal ...

  14. Health-related quality of life among blood donors with hepatitis B and hepatitis C: longitudinal study before and after diagnosis

    OpenAIRE

    Francisco Augusto Porto Ferreira; Cesar de Almeida-Neto; Maria Cristina Dias Teixeira; Edna Strauss

    2015-01-01

    ABSTRACT Introduction: There is evidence that patients suffering from chronic hepatic diseases, including chronic hepatitis B and chronic hepatitis C, have a reduced health-related quality of life. The aim of this study was to evaluate the impact of the notification of test results for hepatitis B and hepatitis C on the quality of life of blood donors. Methods: Over a 29-month period, this study assessed the quality of life of 105 blood donors with positive serological screening tests for ...

  15. High diversity of hepatitis B virus genotypes in Panamanian blood donors: a molecular analysis of new variants.

    Directory of Open Access Journals (Sweden)

    Alexander A Martínez

    Full Text Available Hepatitis B Virus (HBV is an infectious agent that causes more than half of the cases of liver disease and cancer in the world. Globally there are around 250 million people chronically infected with this virus. Despite 16% of the cases of liver disease in Central America are caused by HBV, the information regarding its genetic diversity, genotypes and circulation is scarce. The purpose of this study was to evaluate the genetic variability of the HBV genotypes from HBV-DNA positive samples obtained from screening blood donors at the Social Security System of Panama and to estimate its possible origin. From 59,696 blood donors tested for HBV infection during 2010-2012, there were 74 HBV-DNA positive subjects. Analysis of the partial PreS2-S region of 27 sequences shows that 21% of the infections were caused by genotype A, 3% by genotype D and 76% by genotype F. In addition, we were able to confirm circulation of six sub-genotypes A1, A2, A3, D4, F3, F1 and a proposed new sub-genotype denominated F5pan. We found a confinement of sub-genotypes F1 and F5pan to the western area of Panama. The tMRCA analysis suggests a simultaneous circulation of previously described sub-genotypes rather than recent introductions of the Panamanian sub-genotypes in the country. Moreover, these results highlight the need of more intensive research of the HBV strains circulating in the region at the molecular level. In conclusion, Panama has a high HBV genotype diversity that includes a new proposed sub-genotype, an elevated number of PreCore-Core mutations, and confinement of these variants in a specific geographical location.

  16. Presence of medication taken by blood donors in plasma for transfusion

    NARCIS (Netherlands)

    van Tilborgh-de Jong, A.J.W.; Wiersum-Osselton, J.C.; Touw, D.J.; Schipperus, M.R.

    2015-01-01

    Background and Objectives: The TRIP national hemovigilance and biovigilance office receives reports on side-effects and incidents associated with transfusion of labile blood products. Anaphylactic reactions accounted for the largest number of serious transfusion reactions in the period 2008-2012. In

  17. Renal blood flow using arterial spin labelling MRI and calculated filtration fraction in healthy adult kidney donors pre-nephrectomy and post-nephrectomy

    Energy Technology Data Exchange (ETDEWEB)

    Cutajar, Marica; Clark, Christopher A.; Gordon, Isky [University College London, Imaging and Biophysics Unit, Institute of Child Health, London (United Kingdom); Hilton, Rachel; Olsburgh, Jonathon [Renal Unit, Guy' s and St Thomas' NHS Foundation Trust, London (United Kingdom); Marks, Stephen D. [Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Paediatric Nephrology, London (United Kingdom); Thomas, David L. [University College London, Department of Brain Repair and Rehabilitation, Institute of Neurology, London (United Kingdom); Banks, Tina [Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Radiology, London (United Kingdom)

    2015-08-15

    Renal plasma flow (RPF) (derived from renal blood flow, RBF) and glomerular filtration rate (GFR) allow the determination of the filtration fraction (FF), which may have a role as a non-invasive renal biomarker. This is a hypothesis-generating pilot study assessing the effect of nephrectomy on renal function in healthy kidney donors. Eight living kidney donors underwent arterial spin labelling (ASL) magnetic resonance imaging (MRI) and GFR measurement prior to and 1 year after nephrectomy. Chromium-51 labelled ethylenediamine tetraacetic acid ({sup 51}Cr-EDTA) with multi-blood sampling was undertaken and GFR calculated. The RBF and GFR obtained were used to calculate FF. All donors showed an increase in single kidney GFR of 24 - 75 %, and all but two showed an increase in FF (-7 to +52 %) after nephrectomy. The increase in RBF, and hence RPF, post-nephrectomy was not as great as the increase in GFR in seven out of eight donors. As with any pilot study, the small number of donors and their relatively narrow age range are potential limiting factors. The ability to measure RBF, and hence RPF, non-invasively, coupled with GFR measurement, allows calculation of FF, a biomarker that might provide a sensitive indicator of loss of renal reserve in potential donors. (orig.)

  18. Renal blood flow using arterial spin labelling MRI and calculated filtration fraction in healthy adult kidney donors pre-nephrectomy and post-nephrectomy

    International Nuclear Information System (INIS)

    Renal plasma flow (RPF) (derived from renal blood flow, RBF) and glomerular filtration rate (GFR) allow the determination of the filtration fraction (FF), which may have a role as a non-invasive renal biomarker. This is a hypothesis-generating pilot study assessing the effect of nephrectomy on renal function in healthy kidney donors. Eight living kidney donors underwent arterial spin labelling (ASL) magnetic resonance imaging (MRI) and GFR measurement prior to and 1 year after nephrectomy. Chromium-51 labelled ethylenediamine tetraacetic acid (51Cr-EDTA) with multi-blood sampling was undertaken and GFR calculated. The RBF and GFR obtained were used to calculate FF. All donors showed an increase in single kidney GFR of 24 - 75 %, and all but two showed an increase in FF (-7 to +52 %) after nephrectomy. The increase in RBF, and hence RPF, post-nephrectomy was not as great as the increase in GFR in seven out of eight donors. As with any pilot study, the small number of donors and their relatively narrow age range are potential limiting factors. The ability to measure RBF, and hence RPF, non-invasively, coupled with GFR measurement, allows calculation of FF, a biomarker that might provide a sensitive indicator of loss of renal reserve in potential donors. (orig.)

  19. BLOOD DETECTION OF VOLUNTARY BLOOD DONOR IN TANGSHAN%唐山市无偿献血者血液检测结果分析

    Institute of Scientific and Technical Information of China (English)

    缪维芬; 缪维芳; 董俐坤; 刘璇璇; 韩东颖; 何金奎; 闫振宇

    2016-01-01

    Objective To understand the reasons and developmental trends of blood tests'unqualified rates of Tangshan area in 6 years and to explore the regularity and countermeasure of them ,and to furthest re‐duce blood discarding and save blood resource .Methods A retrospectively statistical analysis was per‐formed on the results of blood donation with Tangshan modern management software from January 2010 to December 2015 in Tangshan ,which is mainly aimed at the blood alanine aminotransferase (ALT) ,hepatitis B surface antigen (HBsAg ) ,hepatitis C virus (anti-HCV ) ,insufficient blood volume and treponema palli‐dum antibody .Results The unqualified rate of local blood donors was 8 .53% ,including HBsAg ,anti -HCV ,anti-HIV ,syphilis and ALT ,which were 0 .33% ,0 .29% ,0 .01% ,0 .48% and 0 .89% ,respective‐ly ,the highest proportion was fat pulp accounted for 5 .72% .The positive rate of anti -HIV was higher in 2015 than that in other years excepted for 2010(P 0 .05) .Conclusion Nearly five years in Tangshan ,the unqualified rate of voluntary blood donor is the highest in 2015 ,the lowest in 2013 .We should strengthen the health care propaganda to reduce the unqualified rates of blood donation .%目的:了解唐山地区6年来血液检测不合格原因及发展趋势,探讨其规律及对策,最大限度地降低血液报废,节约血液资源。方法用唐山现代管理软件回顾性统计分析本地区2010年1月—2015年12月无偿献血者标本检测结果,主要是针对血液中的谷丙转氨酶(ALT)、乙肝表面抗原(HBsAg)、丙型肝炎病毒(抗-HCV)、抗艾滋病病毒抗体(抗-HIV)、血量不足、梅毒螺旋体抗体。结果本地区无偿献血者血液不合格率8.53%,其中HBsAg、抗-HCV、抗-HIV、梅毒和ALT不合格率分别为0.33%、0.29%、0.01%、0.48%、0.89%,脂肪浆占到5.72%,所占比例最高。2015年抗-H IV阳性率高于其他年份( P 0

  20. Tattoos and transfusion-transmitted disease risk: implications for the screening of blood donors in Brazil

    OpenAIRE

    NISHIOKA Sérgio de A.; Gyorkos, Theresa W.; MacLean, J D

    2002-01-01

    Having a tattoo has been associated with serological evidence of hepatitis B and C viruses, as well as human immunodeficiency virus infections and syphilis; all of these are known to be transmissible by blood transfusion. These associations are of higher magnitude for individuals with nonprofessionally-applied tattoos and with two or more tattoos. Tattoos are common among drug addicts and prisoners, conditions that are also associated with transfusion-transmitted diseases. We examined the imp...

  1. Hepatitis B surface antigen variants in voluntary blood donors in Nanjing, China

    OpenAIRE

    Yong-lin Yang; Qiang Fu; Ming-shun Zhang; Jie Cai; Gui-ming Ma; Zu-hu Huang; Xu-bing Cai

    2012-01-01

    Abstract Background Hepatitis B virus (HBV) is still one of the serious infectious risks for the blood transfusion safety in China. One plausible reason is the emergence of the variants in the major antigenic alpha determinant within the major hydrophilic region (MHR) of hepatitis B surface antigen (HBsAg), which have been assumed to evade the immune surveillance and pose a challenge to the disease diagnosis. It is well documented that some commercial ELISA kits could detect the wild-type but...

  2. The breed prevalence of Dog Erythrocyte Antigen 1.1 in the Onderstepoort area of South Africa and its significance in selection of canine blood donors

    OpenAIRE

    L.L. Van der Merwe; L.S. Jacobson; J.G. Pretorius

    2002-01-01

    The blood group antigen Dog Erythrocyte Antigen (DEA) 1.1 is clinically the most important canine blood group as DEA 1.1 antibodies are capable of causing acute haemolytic, potentially life-threatening transfusion reactions. Dogs do not have naturally occurring antibodies to DEA 1.1 but are rapidly sensitised by the first incompatible transfusion. The prevalence of DEA 1.1 in the general dog population is estimated at 42-46 %. Canine blood donors registered with the Onderstepoort Animal Blood...

  3. Identification of anti-PP1P(k) in a blood donor and her family: a case report following her pregnancy and review.

    Science.gov (United States)

    Benidt, Georgette R; Jaben, Elizabeth A; Winters, Jeffrey L; Stubbs, James R

    2010-12-01

    Anti-PP1P(k) is a rare, biphasic antibody with the ability to cause immediate hemolytic transfusion reactions and early spontaneous abortions. The antibody is formed by individuals with the p phenotype. A blood donor with anti-PP1P(k) and the p phenotype was identified through routine donor screening. A sister was found to also be p phenotype. At that time, the sister was 24weeks pregnant. Subsequently, the original blood donor became pregnant. Both individuals were followed throughout their pregnancies and delivered infants without complications from anti-PP1P(k) antibodies. The literature regarding anti-PP1P(k), the p phenotype, and recurrent pregnancy loss in this setting is reviewed. PMID:21030308

  4. Viremic blood donor found by a rapid screening method in a season of high human parvovirus B19 activity in Niterói, Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    Sérgio Setúbal

    2004-02-01

    Full Text Available Erythrovirus B19 infection is usually benign but may have serious consequences in patients with hemolytic anemia (transient aplastic crisis, immunodeficiency (in whom persistent infection can lead to chronic bone marrow failure with anemia, or who are in the first or second trimester of gestation (spontaneous abortion, hydrops fetalis, and fetal death. Being non-enveloped, B19 resists most inactivation methods and can be transmitted by transfusion. B19 is difficult to cultivate and native virus is usually obtained from viremic blood. As specific antibodies may be absent, and there is no reliable immunological method for antigen detection, hybridization or polymerase chain reaction are needed for detecting viremia. A rapid method, gel hemagglutination (Diamed ID-Parvovirus B19 Antigen Test, can disclose highly viremic donations, whose elimination lessens the viral burden in pooled blood products and may even render them non-infectious. In order to obtain native antigen and to determine the frequency of viremic donors, we applied this test to blood donors in a period of high viral activity in our community. Positive or indeterminate results were re-tested by dot-blot hybridization. We tested 472 donors in 1998 and 831 ones in 1999. One viremic donor was found in 1999. We suggest that in periods of high community viral activity the gel hemagglutination test may be useful in avoiding highly viremic blood being added to plasma pools or directly transfused to patients under risk.

  5. Frequency of the hemochromatosis HFE mutations C282Y, H63D, and S65C in blood donors in the Faroe Islands

    DEFF Research Database (Denmark)

    Milman, Nils; á Steig, Torkil; Koefoed, Pernille;

    2004-01-01

    The aim of the study was to assess the frequencies of the hereditary hemochromatosis HFE mutations C282Y, H63D, and S65C in the population in the Faroe Islands. The series comprised 200 randomly selected blood donors of Faroese heritage. The frequency of the C282Y, H63D, and S65C mutations...

  6. True positivity of anti-Hepatitis C Virus Enzyme-linked immunosorbent assay reactive blood donors: A prospective study done in western India

    Directory of Open Access Journals (Sweden)

    Sunita Tulsiani

    2012-01-01

    Full Text Available Background: A significant number of safe donations are removed from the blood supply, because of the reactive anti-HCV screening test results. This study aimed to assess if the HCV (Hepatitis C Virus seropositive donors were confirmed positive or not. Materials and Methods: More than 68,000 blood donors′ samples were routinely screened and 140 samples were found to be anti-HCV ELISA reactive. These 140 samples were tested by NAT. The NAT negative samples were tested by RIBA. Analysis of samples reactive in single ELISA kit vs. two ELISA kits was done. Results: Out of 140 anti-HCV ELISA reactive samples, a total of 16 (11.43% were positive by NAT. The results of 124 RIBA showed 6 (4.84% positive, 92 (74.19% negative, and 26 (20.97% indeterminate results. None of the sample which was reactive in only single ELISA kit was positive by NAT or RIBA. Conclusion: Only a minority of blood donors with repeatedly reactive anti-HCV screening test is positive by confirmatory testing, but all these blood units are discarded as per existing legal provisions in India. Efforts should be made to retain these donors and also donor units.

  7. [Absence of HTLV-I/II virus circulation in blood donors from the provinces of Santa Fe and Santiago del Estero].

    Science.gov (United States)

    Gastaldello, R; Fazzola, P; Caeiro, L; Maturano, E; Perez De Rosas, A; Fernandez, I; Racca, R; Rodriguez, M B; Molina, M; Alonso, S; Gallego, S

    2002-01-01

    In the present study, we evaluated the seroprevalence of HTLV-I/II infection among the blood donors in Santa Fe and Santiago del Estero provinces. A total of 1327 serum samples from blood donors from Rafaela blood bank of Santa Fe province and 3382 serum samples from blood donors from Dr Edgar Bouzon blood bank of Santiago del Estero province were studied. The antibody screening was done by particle agglutination assay (PA) (SERODIA, Fujirebio Inc., Tokyo, Japan) or by enzyme immuno assay (EIA) (Abbott HTLV-I/HTLV-II EIA, Abbott, Germany). The "in house" indirect immunofluorescence assay (IFA) and Western blot (Bioblot HTLV Biokit, Barcelona, Spain) were used as confirmatory assays. All the samples resulted negative for specific antibodies against HTLV-I/II. These results suggest that HTLV-I/II are not circulating in low risk populations in these provinces or that the prevalences of infection would be lower than that reported by blood banks in other provinces of Argentina.

  8. Comparative outcomes between cord blood transplantation and bone marrow or peripheral blood stem cell transplantation from unrelated donors in patients with hematologic malignancies: a single-institute analysis

    Institute of Scientific and Technical Information of China (English)

    CHEN Yu-hong; XU Lan-ping; LIU Dai-hong; CHEN Huan; ZHANG Xiao-hui; HAN Wei; WANG Feng-rong

    2013-01-01

    Background Umbilical cord blood (UCB) has grown substantially as an alternative source of hematopoietic stem cells for unrelated donor transplantation in both adult and pediatric patients.Our aim was to assess the leukemia-free survival (LFS) and some primary results,such as hematologic recovery,risk of graft-versus-host disease (GVHD),relapse,and long-term survival,after unrelated cord blood transplantation compared with the outcomes of transplantations from other unrelated graft source.Methods The clinical outcomes of 112 consecutive patients with acute leukemia who received umbilical cord blood (UCBT) as a primary unrelated stem cell source (n=38),bone marrow (UBMT n=28,transplanted before January 2003),or peripheral blood stem cells (UPBSCT n=46,transplanted after January 2003) between July 2000 and July 2008 were analyzed.Results Except that the patients were much younger in the UCBT group (median age,10.5 years in UCBT,30 years in UPBSCT,and 20 years in UBMT),other pre-transplant parameters,such as gender,diagnosis,and the phase of disease,were comparable.All patients received myeloablative regimens,primarily including BUCY; however,there was less antithymocyte globulin (ATG) used for the UBMT patients (2138 in UCBT,0/46 in UPBSCT,and 8/28 in UBMT did not use ATG,P=0.000).Significant delays in engraftment occurred after UCBT for both neutrophil cells and platelets.The cumulative allo-engraftment rates were also significantly lower (87.8% vs.97.8% vs.100% for WBC,P=0.000; 73.0% vs.97.5% vs.89.5% for PLT,P=0.000) for UCBT.The incidence of Grade 2-4 and 3-4 acute graft versus host disease (aGVHD) was much higher in the UBMT group but did not differ among the other groups (51% and 13.2%,40.2% and 10.5%,and 77.4% and 41.2%,respectively,for UCBT,UPBSCT,and UBMT,P=0.000).The occurrence of extensive chronic GVHD (cGVHD)was significantly decreased for recipients of UCBT (4%) compared with that of UPBSCT (39.1%) and UBMT (49.1%,P=0

  9. Avaliação da fidedignidade de um instrumento voltado à satisfação do doador de sangue Reliability of a blood donor satisfaction questionnaire

    Directory of Open Access Journals (Sweden)

    Vera Lucia Borges

    2005-06-01

    Full Text Available OBJETIVOS: O objetivo do presente estudo é avaliar a fidedignidade de um instrumento destinado a mensurar a satisfação de doadores de sangue em um centro de hematologia e transfusão. SUJEITOS E MÉTODOS: Foi desenvolvido um questionário objetivando mensurar a satisfação dos doadores de sangue do Hemocentro/Ribeirão Preto, onde os mesmos classificaram 18 afirmativas em uma escala de Likert de 4 itens, variando de "totalmente errado" a "totalmente certo". Uma amostra de 407 doadores foi obtida sistematicamente, onde, a cada quatro pessoas que seqüencialmente compareceram para doação, o quarto era convidado a preencher o questionário. Uma amostra final de 407 doadores foi utilizada para a análise da fidedignidade do questionário e análises preliminares. A análise de correspondência (AC foi utilizada para avaliação do grau de satisfação dos doadores para cada item. RESULTADOS: Os participantes tinham em média 31,2 anos (desvio padrão de 9,13 e amplitude de 18 a 67 anos. Foi encontrado um coeficiente alfa de Cronbach de 0,97, indicando uma consistência interna muito alta do questionário. A AC indicou que os itens com maior insatisfação são o acesso ao Hemocentro e o tempo gasto na doação. Itens de maior satisfação referem-se à confiança no serviço, funcionários e atendimento. A AC identificou que, de modo geral, as pessoas que estão doando sangue pela primeira vez tendem a uma menor satisfação. CONCLUSÃO: A alta consistência interna do questionário indica que os itens abordados têm alto poder de mensurar um mesmo objeto, mas ainda há a necessidade de estudar a validação do instrumento para avaliar se tal objeto é necessariamente a satisfação do doador.OBJECTIVES: The purpose of this study was to evaluate the reliability of a self-reporting instrument to measure the Portuguese-speaking blood donor satisfaction in a hematology and blood transfusion center. SUBJECTS AND METHODS: Data were collected from

  10. Testing for Partial RhD with a D-Screen Diagast Kit in Moroccan Blood Donors with Weak D Expression.

    Science.gov (United States)

    Kabiri, Z; Benajiba, M; Hajjout, K; Dakka, N; Bellaoui, H

    2014-01-01

    The aim of this study was to search for the partial D phenotype in Moroccan blood donors with weak D expression. The study included 32 samples with weak D phenotype, and partial D category red blood cells were detected with the D-Screen Diagast kit, which consists in 9 monoclonal anti-D antibodies specific for the most common categories of partial D. Among the 32 samples studied, we identified 13 specific reactions to a partial D antigen (3 DVI, 2 DVa, 2 DIII((a,b,c)), and 6 DVII), with 8 reactions suggesting a weak D and 11 reactions providing no formal argument in favor of a partial D antigen. This work can be used to validate the performance of the anti-D reagent and to improve the safety of transfusion of red blood cells from donors expressing the partial D antigen by integrating the finding into the recipient file with a recommendation concerning the appropriate care. PMID:25530908

  11. Data on how several physiological parameters of stored red blood cells are similar in glucose 6-phosphate dehydrogenase deficient and sufficient donors.

    Science.gov (United States)

    Tzounakas, Vassilis L; Kriebardis, Anastasios G; Georgatzakou, Hara T; Foudoulaki-Paparizos, Leontini E; Dzieciatkowska, Monika; Wither, Matthew J; Nemkov, Travis; Hansen, Kirk C; Papassideri, Issidora S; D'Alessandro, Angelo; Antonelou, Marianna H

    2016-09-01

    This article contains data on the variation in several physiological parameters of red blood cells (RBCs) donated by eligible glucose-6-phosphate dehydrogenase (G6PD) deficient donors during storage in standard blood bank conditions compared to control, G6PD sufficient (G6PD(+)) cells. Intracellular reactive oxygen species (ROS) generation, cell fragility and membrane exovesiculation were measured in RBCs throughout the storage period, with or without stimulation by oxidants, supplementation of N-acetylcysteine and energy depletion, following incubation of stored cells for 24 h at 37 °C. Apart from cell characteristics, the total or uric acid-dependent antioxidant capacity of the supernatant in addition to extracellular potassium concentration was determined in RBC units. Finally, procoagulant activity and protein carbonylation levels were measured in the microparticles population. Further information can be found in "Glucose 6-phosphate dehydrogenase deficient subjects may be better "storers" than donors of red blood cells" [1]. PMID:27437434

  12. Blood donation before surgery

    Science.gov (United States)

    ... type of donor blood. Many communities have a blood bank where healthy people can donate blood. This blood ... need to arrange with your hospital or local blood bank before your surgery to have directed donor blood. ...

  13. Negative association of donor age with CD34+ cell dose in mixture allografts of G-CSF-primed bone marrow and G-CSF-mobilized peripheral blood harvests

    Institute of Scientific and Technical Information of China (English)

    Li Yan; Chang Yingjun; Xu Lanping; Zhang Xiaohui; Huang Xiaojun

    2014-01-01

    Background The effects of donor characteristics on CD34+ cell dose remain controversial.Recently,we developed a novel haploidentical transplant protocol,in which mixture allografts of granulocyte colony-stimulating factor (G-CSF)-primed bone marrow (G-BM) and G-CSF-mobilized peripheral blood (G-PB) were used.The aim of this study was to investigate the effects of donor characteristics on CD34+ cell dose in mixture allografts of G-BM and G-PB.Methods A total of 162 healthy adult donors,who underwent bone marrow harvest and peripheral blood collection between January 2009 and November 2010 in Peking University People's Hospital,were prospectively investigated.G-CSF was administered subcutaneously at a dose of 5 μg/kg once a day for 5-6 consecutive days.Bone marrow and peripheral blood stem cells were harvested on the fourth day and fifth day,respectively.A final total CD34+ cell dose less than 2× 106 cells/kg recipient body weight was considered a poor mobilization.Results Of the 162 donors,31 (19.1%) did not attain this threshold.The obtained median CD34+ cell doses in bone marrow,peripheral blood,and mixture allografts were 0.83×106/kg,2.40×106/kg,and 3.47×106/kg,respectively.Multiple regression analysis showed that donor age had a significant negative effect on CD34+ cell dose in either G-BM,or G-PB,or mixture allografts of G-BM and G-PB.And a 1-year increase in age was associated with a 5.6% decrease in the odds of achieving mobilization cutoff.No significant correlation was found for donor gender,body mass index (BMI),and weight.Conclusion Donor age is the only factor among the four parameters,including age,gender,weight,and BMI,that influence CD34+ cell dose in mixture allografts of G-BM and G-PB,and younger donors should be chosen to obtain sufficient CD34+ cells for transplantation.

  14. Thymic function recovery after unrelated donor cord blood or T-cell depleted HLA-haploidentical stem cell transplantation correlates with leukemia relapse

    OpenAIRE

    Clave, Emmanuel; Lisini, Daniela; Douay, Corinne; Giorgiani, Giovanna; Busson, Marc; Zecca, Marco; Moretta, Francesca; Acquafredda, Gloria; Brescia, Letizia P.; Locatelli, Franco; Toubert, Antoine

    2013-01-01

    Use of alternative donors/sources of hematopoietic stem cells (HSC), such as cord blood (CB) or HLA-haploidentical (Haplo)-related donors, is associated with a significant delay in immune reconstitution after transplantation. Long-term T-cell immune reconstitution largely relies on the generation of new T cells in the recipient thymus, which can be evaluated through signal joint (sj) and beta T-cell-Receptor Excision Circles (TREC) quantification. We studied two groups of 33 and 24 children r...

  15. 山东省枣庄市无偿献血者艾滋病知识调查%AIDS knowledge survey of unpaid blood donors in Zaozhuang

    Institute of Scientific and Technical Information of China (English)

    赵燕

    2014-01-01

    questionnaire on the spot 3 576(93.08%).Questionnaire on AIDS knowledge,attitude and effective was 3 358(87.40%);The sexual behavior effective questionnaire was 1 201(31.26%).The original data was put into Excellto statistical analysis and processing .Results The situation of understanding the knowledge of HIV/AIDS,understanding two ways of blood and sexually transmitted were 86.52%and 80.23% respectively;Understanding of mother-to-child vertical transmission accuracy was only 50.67%;The right to use condoms to prevent the spread of HIV was 74.71%;Accuracy up to 74.75%of prostitution susceptible to HIV/AIDS;Patients who were only prevented but couldn′t be cured of HIV was 93.26%.Blood donors before dona-ting blood,donate blood once and donate twice correct awareness were significantly improved .About the HIV/AIDS can infectious,blood transfusion could pass and be transmitted through sexual transmission ,mother-to-child transmis-sion,embrace or kissing transmission ,intravenous drug use ,condom could prevent ,could be cure the recognition accu-racy rate was(26.57%,64.51%,78.83%),(51.54%,80.12%,89.61%),(61.41%,73.57%,86.82%), (59.56%,77.63%,88.79%),(30.54%,68.48%,72.40%),(59.83%,70.21%,85.62%),(64.58%, 81.27%,93.56%),(68.59%,84.63%,85.28%),Before the blood supply and after donate blood twice the aware-ness for HIV/AIDS were significantly increased,there was a positive correlation between(all P<0.05).Unpaid blood donation AIDS knowledge source of propaganda materials and donating blood (43.67%),newspaper and magazine (28.12%),radio and television(2.71%),5.60% family friends and colleagues.Conclusion Unpaid donors to master a certain knowledge of HIV/AIDS;Carry out AIDS health education and intervention of epidemiology ,is to en-sure that the citizens to donate blood safety preconditions .

  16. Relationship between the prevalence of hemoglobin S and the ethnic background of blood donors in Paraná state

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    Kárita C. F. Lidani

    2015-08-01

    Full Text Available ABSTRACTIntroduction:Hemoglobin S (HbS is one of the most common inherited hematological disorders in humans. In Brazil, the sickle-cell disease (SCD has significant epidemiological importance due to its prevalence and the morbidity-mortality and, therefore, it has been identified as a matter of public health.Objective:To determine the prevalence of HbS among Asian Brazilian, Afro Brazilian, and Euro Brazilian individuals of a blood bank in Curitiba.Material and method:The study was conducted from January 2008 to December 2009, and included 83,213 donors seen at the Instituto Paranaense de Hemoterapia e Hematologia.Results and discussion:The prevalence of HbS in the studied population was 0.9%, among them, 0% Asian Brazilians, 2.7% Afro Brazilians, and 0.7% Euro Brazilians. There was a positive association, statistically significant for the sickle cell trait in Afro-descendants, with odds ratio (OR 4.01; confidence interval (CI 3.42-4.72; and 95% confidence.Conclusion:This study showed higher rates of sickle cell trait in Afro Brazilians, which corroborates data published in other Brazilian regions and states.

  17. Assessment of 188Re marked anti MHC class Ⅱ antibody by peripheral blood mononuclear cells stimulated by donor alloantigen

    Institute of Scientific and Technical Information of China (English)

    DING Guo-ping; CAO Li-ping; LIU Jie; LIU Da-ren; QUE Ri-sheng; ZHU Lin-hua; ZHOU Yi-ming; MAO Ke-jie; HU Jun-an

    2011-01-01

    Background Previous studies showed that anti MHC-Ⅱ monoclone antibody (MAb) only had partial inhibiting effect of alloreactive mixed lymphocyte reaction (MLR) in vitro and it was unsteady and non-persistent. The aim of this research was to determine whether radioactive isotope 188Re marked MHC-Ⅱ antibody could benefit the allograft acceptance in transplantation as compared to normal MHC-Ⅱ antibody.Methods 188Re was incorporated to 2E9/13F(ab')2 which is against swine MHC class Ⅱ antigen (MAb-188Re). Porcine peripheral blood mononuclear (PBMC) cells were examined for proliferation and cytokine mRNA expression after stimulation with MHC-Ⅱ MAb or MAb-188Re.Results The proliferative response of recipient PBMCs in mixed lymphocyte reaction (MLR) to donor alloantigen showed that the stimulation index of MAb-188Re group was significantly lower than the MHC-Ⅱ MAb group and control (P<0.05). mRNA expression of interleukin 2, interferon Y and tumor necrosis factor α (type 1 cytokines) was lower in MAb-188Re group than the MHC-Ⅱ MAb group, while interleukin 10 (type 2 cytokines) was higher in MAb-188Re group in the first 24 hours.Conclusion MAb-188Re could help the graft acceptance by inhibiting T cell proliferation, lowering the expression of type 1 cytokines and elevating the type 2 cytokines produced by PBMC.

  18. FCGR3B gene frequencies among ethnic Thai blood donors from a regional hospital in Eastern Thailand.

    Science.gov (United States)

    Kongmaroeng, C; Kumkaen, K

    2015-02-01

    The FCGR3B gene encodes three human neutrophil antigens which consist of HNA-1a, HNA-1b, and HNA-1c. These antigens are encoded by three alleles in the FCGR3B locus: FCGR3B*01, FCGR3B*02, and FCGR3B*03 alleles, respectively. The frequencies of FCGR3B alleles have been reported in different ethnic populations. This study compared the FCGR3B gene frequencies among 230 unrelated healthy Eastern Thai blood donors in Rayong hospital with the previously published studies. The polymerase chain reaction-sequence-specific primers method was performed to determine FCGR3B genotypes. The results showed that the allele frequencies of FCGR3B*01, FCGR3B*02, and FCGR3B*03 were 0.722, 0.274, and 0.009, respectively. The FCGR3B*01 and FCGR3B*02 frequencies found in the Eastern Thais were similar to the previous reports investigating in Northern Thais, Chinese Han, Taiwanese, and Japanese populations. Interestingly, our data showed statistically significant difference (P American, German, and Italian populations. In addition, one FCGR3Bnull , which represents a gene deletion, was also found in this study. This information is important not only for the assessment of neutrophil antibody-mediated clinical conditions and for disease association studies but also for anthropological studies.

  19. Analysis of HCV genotypes from blood donors shows three new HCV type 6 subgroups exist in Myanmar.

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    Shinji T

    2004-06-01

    Full Text Available The prevalence of hepatitis C virus (HCV genotypes in Myanmar in comparison with the rest of Southeast Asia is not well known. Serum samples were obtained from 201 HCV antibody-positive volunteer blood donors in and around the Myanmar city of Yangon. Of these, the antibody titers of 101 samples were checked by serial dilution using HCV antibody PA test II and Terasaki microplate as a low-cost method. To compare antibody titers by this method and RNA identification, we also checked HCV-RNA using the Amplicor 2.0 test. Most high-titer groups were positive for HCV-RNA. Of the 201 samples, 110 were successfully polymerase chain reaction (PCR amplified. Among them, 35 (31.8% were of genotype 1, 52 (47.3% were of genotype 3, and 23 (20.9% were of type 6 variants, and phylogenetic analysis of these type 6 variants revealed that 3 new type 6 subgroups exist in Myanmar. We named the subgroups M6-1, M6-2, and M6-3. M6-1 and M6-2 were relatively close to types 8 and 9, respectively. M6-3, though only found in one sample, was a brand-new subgroup. These subtypes were not seen in Vietnam, where type 6 group variants are widely spread. These findings may be useful for analyzing how and when these subgroups were formed.

  20. Changes of metabolic syndrome related indexes before and after blood donation in blood donors%献血前后代谢综合征相关血液指标变化

    Institute of Scientific and Technical Information of China (English)

    蔡珍; 罗琼

    2015-01-01

    Objective To investigate the significance of blood donation in preventing metabolic syndrome (MS)by analyzing the changes of the blood pressure and the related biochemical indexes before and after blood donation in the blood donors with high risk of MS.Methods The blood specimens of 102 blood donors,aged 45 -55 years old with the systolic blood pressure of 110 -140 mmHg and the diastolic blood pressure of 75 -90 mmHg,the body mass index> 24 kg/m2 ,were selected as the subjects.The blood pressure,heart rate,iron reserves,blood glucose,blood lipids,and the other indicators were determined before blood donation. All the above indicators were tested again on 42 d after blood donation.Results The blood pressure,iron reserves,blood glucose and LDL/HDL ratio after donation showed significant changes compared with before blood donation(P <0.05 ).Conclusion The changes of the MS related blood indexes exist before and after blood donation,blood donation has a certain significance for preven-ting MS in the blood donors with high risk of MS.%目的:分析代谢综合征(MS)的高危献血者献血前后的血压及相关生化指标来探讨献血对预防 MS 的意义。方法研究对象为男性或绝经后女性共102例,年龄45~55岁,收缩压110~140 mm Hg,舒张压75~90 mm Hg,体质量指数大于24 kg/m2,献血前测定血压、心率、铁储量、血糖、血脂等指标与献血42 d 后再测定相同指标进行比较。结果血压、铁储量、血糖、低密度脂蛋白/高密度脂蛋白(LDL/HDL)与献血前比较差异有统计学意义(P <0.05)。结论献血前后 MS 相关血液指标存在变化,献血对于 MS 高危者预防 MS 具有一定意义。