Direkbusarakom, S.; Ruangpan, L.; Ezura, Y.; Yoshimizu, M.
The leaves of Clinacanthus nutans Lindua, a well-known Thai traditional medicine against viral disease in human being, were used to extract antiviral substances by ethanol using soxhlet apparatus. The extract was tested for its activity against yellow-head rhabdovirus (YRV) in black tiger shrimp (Penaeus monodon). Virucidal effect was investigated by observation of mortality of the shrimp injected with the extract-treated virus. The results showed that extract of C. nutans inhibited YRV in vi...
Tassanee Eamkamon; Sirawut Klinbunga; Kumthorn Thirakhupt; Piamsak Menasveta; Narongsak Puanglarp
Acute toxicity and neurotoxicity of chlorpyrifos were determined in black tiger shrimp, P. monodon. LC50 values after 24 to 96 h of exposure were between 149.55 and 59.16 nmol/L. To determine the neurotoxicity of chlorpyrifos, the inhibition of acetylcholinesterase was monitored in the gill of the shrimps exposed to lethal (0.019, 0.194, and 1.942 µmol/L) and sub-lethal (0.019, 0.194, and 1.942 nmol/L) concentrations of chlorpyrifos. In lethal dose exposure, the AChE activities observed in sh...
Thi Tu, H.; Silvestre, F.; A. Bernard; Douny, C.; Thanh Phuong, N.; Tai Tao, C.; Maghuin-Rogister, G; KESTEMONT P.
In Vietnam, enrofloxacin is one of the most commonly used antibiotics in shrimp farms. Although the European Union set a maximum residue limit (MRL) of 100 µg/kg for enrofloxacin and its metabolite ciprofloxacin in edible tissues of aquatic animals for human consumption, only few data are available on its potential deleterious effects on shrimp. This study aimed to investigate the impacts of enrofloxacin on oxidative stress in hepatopancreas and gills of black tiger shrimp (Penaeus monodon). ...
Rungrassamee, Wanilada; Klanchui, Amornpan; Maibunkaew, Sawarot; Karoonuthaisiri, Nitsara
The intestinal microbiota play important roles in health of their host, contributing to maintaining the balance and resilience against pathogen. To investigate effects of pathogen to intestinal microbiota, the bacterial dynamics upon a shrimp pathogen, Vibrio harveyi, exposures were determined in two economically important shrimp species; the black tiger shrimp (BT) and the Pacific white shrimp (PW). Both shrimp species were reared under the same diet and environmental conditions. Shrimp survival rates after the V. harveyi exposure revealed that the PW shrimp had a higher resistance to the pathogen than the BT shrimp. The intestinal bacterial profiles were determined by denaturing gradient gel electrophoresis (DGGE) and barcoded pyrosequencing of the 16S rRNA sequences under no pathogen challenge control and under pathogenic V. harveyi challenge. The DGGE profiles showed that the presence of V. harveyi altered the intestinal bacterial patterns in comparison to the control in BT and PW intestines. This implies that bacterial balance in shrimp intestines was disrupted in the presence of V. harveyi. The barcoded pyrosequencing analysis showed the similar bacterial community structures in intestines of BT and PW shrimp under a normal condition. However, during the time course exposure to V. harveyi, the relative abundance of bacteria belong to Vibrio genus was higher in the BT intestines at 12h after the exposure, whereas relative abundance of vibrios was more stable in PW intestines. The principle coordinates analysis based on weighted-UniFrac analysis showed that intestinal bacterial population in the BT shrimp lost their ability to restore their bacterial balance during the 72-h period of exposure to the pathogen, while the PW shrimp were able to reestablish their bacterial population to resemble those seen in the unexposed control group. This observation of bacterial disruption might correlate to different mortality rates observed between the two shrimp species
Full Text Available Acute toxicity and neurotoxicity of chlorpyrifos were determined in black tiger shrimp, P. monodon. LC50 values after 24 to 96 h of exposure were between 149.55 and 59.16 nmol/L. To determine the neurotoxicity of chlorpyrifos, the inhibition of acetylcholinesterase was monitored in the gill of the shrimps exposed to lethal (0.019, 0.194, and 1.942 µmol/L and sub-lethal (0.019, 0.194, and 1.942 nmol/L concentrations of chlorpyrifos. In lethal dose exposure, the AChE activities observed in shrimp exposed to 0.194, and 1.942 µmol/L of chlorpyrifos were significantly lower (1.7 and 3.3 times than that of control shrimp after 30 min of exposure (p<0.05. In sub-lethal exposure tests, the AChE activity of shrimp was significantly lower (1.9 times than that of control shrimp after exposure to 1.942 nmol/L of chlorpyrifos for 72 h (p<0.05. The sensitive reduction of AChE activity at the sub-lethal concentration, which was 30 times lower than 96 h LC50 value found in this study, indicates the potential use as a biomarker of chlorpyrifos exposure.
Full Text Available Effects of dietary pigment on survival and disease (white spot syndrome virus: WSSV resistance in black tiger shrimp post larva (Penaeus monodon, Fabricius (PL15 for a 30-day period were studied. The results showed that not only was mean survival of black tiger shrimp (PL15 fed with supplementation of Lucarotene or Betatene at 125 mg/kg diet significantly higher (P<0.05 but also the body color was increased. There were no effects of dietary pigment on mean weight, percent weight gain and WSSV resistance. However, mean WSSV resistance of black tiger shrimp (PL15 fed diet containing Lucantin pink 50 mg/kg diet, Spirulina 30 g/kg diet or Betatene 125 mg/kg diet was higher than that of control.
Braak, van de C.B.T.
Tropical shrimp culture is one of the fastest growing aquaculture sectors in the world. Since this production sector is highly affected by infectious pathogens, disease control is nowadays a priority. Effective prevention methods can be developed more efficiently when quantitative assays for the eva
Rungrassamee, Wanilada; Maibunkaew, Sawarot; Karoonuthaisiri, Nitsara; Jiravanichpaisal, Pikul
This study investigates an effect of bacterial lipopolysaccharide (LPS) as feed supplement to improve immunity of the black tiger shrimp (Penaeus monodon). LPS was coated to commercial feed pellets and given to the shrimp once or twice a day for 10 days before an exposure with shrimp pathogenic bacterium Vibrio harveyi. The growth rates, percent weight gains, total hemocyte and granulocyte counts and survival rates of shrimp between the LPS-coated pellet fed groups and a control group where shrimp fed with commercial feed pellets were compared. After 10 days of the feeding trials, growth rates were not significantly different in all groups, suggesting no toxicity from LPS supplement. To determine beneficial effect of LPS diets, each group was subsequently exposed to V. harveyi by immersion method and the survival rates were recorded for seven days after the immersion. Regardless of the dosages of LPS, the shrimp groups fed with LPS-coated pellets showed higher survival rates than the control group. There was no significant difference in survival rates between the two LPS dosages groups. In addition to survival under pathogen challenge, we also determine effect of LPS on immune-related genes after 10-day feeding trial. Gene expression analysis in the P. monodon intestines revealed that antilipopolysaccharide factor isoform 3 (ALF3), C-type lectin, and mucine-like peritrophin (mucin-like PM) were expressed significantly higher in a group fed with LPS supplemental diet once or twice a day than in a control group. The transcript levels of C-type lectin and mucin-like PM had increased significantly when LPS was given once a day, while significant induction of ALF3 transcripts was observed when shrimp were fed with LPS twice a day. The up-regulation of the immune gene levels in intestines and higher resistance to V. harveyi of the shrimp fed with LPS provide the evidence for potential application of LPS as an immunostimulant in P. monodon farming. PMID:23751331
Full Text Available Intestinal bacterial communities in aquaculture have been drawn to attention due to potential benefit to their hosts. To identify core intestinal bacteria in the black tiger shrimp (Penaeus monodon, bacterial populations of disease-free shrimp were characterized from intestines of four developmental stages (15-day-old post larvae (PL15, 1- (J1, 2- (J2, and 3-month-old (J3 juveniles using pyrosequencing, real-time PCR and denaturing gradient gel electrophoresis (DGGE approaches. A total of 25,121 pyrosequencing reads (reading length = 442±24 bases were obtained, which were categorized by barcode for PL15 (7,045 sequences, J1 (3,055 sequences, J2 (13,130 sequences and J3 (1,890 sequences. Bacteria in the phyla Bacteroides, Firmicutes and Proteobacteria were found in intestines at all four growth stages. There were 88, 14, 27, and 20 bacterial genera associated with the intestinal tract of PL15, J1, J2 and J3, respectively. Pyrosequencing analysis revealed that Proteobacteria (class Gammaproteobacteria was a dominant bacteria group with a relative abundance of 89% for PL15 and 99% for J1, J2 and J3. Real-time PCR assay also confirmed that Gammaproteobacteria had the highest relative abundance in intestines from all growth stages. Intestinal bacterial communities from the three juvenile stages were more similar to each other than that of the PL shrimp based on PCA analyses of pyrosequencing results and their DGGE profiles. This study provides descriptive bacterial communities associated to the black tiger shrimp intestines during these growth development stages in rearing facilities.
Full Text Available A crustin gene (Amk1 was identified from a haemocyte library of the black tiger shrimp, Penaeus monodon. The full-length cDNA consists of 411 bp encoding a deduced precursor of 136 amino acids with a signal peptide of 17 aminoacids. Amk1 contains a hydrophobic and a Gly-rich region at the N-terminus and a 12 conserved cysteine domain (6-DSC at the C-terminus. Transcripts of Amk1 are mainly detected in haemocytes and gills by RT-PCR analysis. A recombinant Amk1was overexpressed and purified from Escherichia coli. This has a molecular mass of 43.66 kDa with a predicted pI of 8.23. Antibacterial assays demonstrated that recombinant Amk1 exhibited antibacterial activity against Gram-positive and Gramnegativebacteria with strong inhibition against Staphylococcus aureus and Vibrio harveyi.
Studies were conducted on contamination by Salmonella and pathogenic Vibrio spp. in samples of aquaculture black tiger shrimp, the water supply (canal water which is supplied as raw pond water before treatment), pond water, feed materials, and fresh and frozen shrimp. Salmonella was detected in samples of the water supply, pond water, feed materials, fresh shrimp at farm, fresh shrimp from wholesale market and frozen shrimp destined for export at levels of 13.95%, 1.53%, 1.14%, 3.17%, 30.4% and 0.21% respectively. V. cholerae non 01 was found in one sample of water from a culture pond of 131 tested (0.8%). V. parahaemolyticus was found in samples of canal water, pond water, fresh black tiger shrimp collected at farms, fresh black tiger shrimp collected at wholesale shrimp markets and frozen black tiger shrimp destined for export at levels of 2.3%, 5.3%, 14.3%, 48 % and 0.2% respectively. The strains identified as V. parahaemolyticus were examined for the presence or absence of the TDH and TRH. The incidence of TDH (KP+) was 2.67% (seven of 262 strains) and of TRH (urease reaction) was 1.15% (three of 262 strains). Salmonella and V. parahaemolyticus were found in a high percentage in fresh black tiger shrimp collected from wholesale shrimp markets. These shrimp are used as raw material for domestic consumption and for processing for exported shrimp products. Therefore GMP and/or Hazard Analysis Critical Control Point (HACCP) system for shrimp distributors/producers should be applied. (author)
Vatanavicharn, Tipachai; Pongsomboon, Siriporn; Tassanakajon, Anchalee
Two isoforms of plasmolipin were initially identified from the black tiger shrimp (Penaeus monodon) EST database and completed using 50 RACE to reveal complete cDNAs of 558 bp (PmPLP1) and 537 bp(PmPLP2) with 87% nucleotide sequence identity. The deduced amino acid sequences contained four-transmembrane domains and showed the highest amino acid identity (49% and 51%, respectively) to the honey bee (Apis mellifera) chemokine-like factor (CKLF), with a very similar hydrophobic pattern to other plasmolipins. Transcripts of PmPLP1 and PmPLP2 were observed in all tested shrimp tissues with the highest expression levels in the gill and epipodite for PmPLP1 and in the hemocytes and antennal gland for PmPLP2. PmPLP1 transcript levels were significantly upregulated in hemocytes at 24 and 72 h post infection (hpi) with yellow head virus (YHV) (7.4- and 14.7- fold, respectively), but only after 72 hpi by white spot syndrome virus (WSSV). In contrast, PmPLP2 was only slightly (but statistically significant)up-regulated with YHV and WSSV. Thus, PmPLPs have the potential to be a part of viral infection mechanisms or defense response. This is the first characterization of a plasmolipin gene in crustaceans. PMID:22766100
Full Text Available This study aimed to determine the effect of the twig extract of the apple mangrove Sonneratia caseolaris on the activities of trypsin, chymotrypsin and lipase in the postlarval black tiger shrimpPenaeus monodon. Incorporating twig extracts of the apple mangrove S. caseolaris increased the activityof trypsin in juvenile P. monodon when fed at three to four times daily. When fed twice daily, trypsinactivity was at the same level as that in shrimp fed the control diet (without medication. The extractresulted in the enhanced chymotrypsin activity starting when shrimps were fed medicated diet twice dailyuntil four times daily. Lipase activity was not at all affected by the apple mangrove extract. Thesefindings demonstrated that the twig apple mangrove extract could be used as aprophylactic/therapeutant and was not deleterious to the nutrition of the black tiger shrimp; in fact, itstimulated protein digestion.
Sarmiento, Katreena P; Panes, Vivian A; Santos, Mudjekeewis D
Chitin deacetylases have been identified and studied in several fungi and insects but not in crustaceans. These glycoproteins function in catalyzing the conversion of chitin to chitosan by the hydrolysis of N-acetamido bonds of chitin. Here, for the first time, the full length cDNA of chitin deacetylase (CDA) gene from crustaceans was fully cloned using a partial fragment obtained from a transcriptome database of the gills of black tiger shrimp Penaeus monodon that survived White Spot Syndrome Virus (WSSV) infection employing Rapid Amplification of cDNA Ends (RACE) PCR. The shrimp CDA, named PmCDA1, was further characterized by in silico analysis, and its constitutive expression determined in apparently healthy shrimp through reverse transcription PCR (RT-PCR). Results revealed that the P. monodon chitin deacetylase (PmCDA1) is 2176 bp-long gene with an open reading frame (ORF) of 1596 bp encoding for 532 amino acids. Phylogenetic analysis revealed that PmCDA1 belongs to Group I CDAs together with CDA1 and CDA2 proteins found in insects. Moreover, PmCDA1 is composed of a conserved chitin-binding peritrophin-A domain (CBD), a low-density lipoprotein receptor class A domain (LDL-A) and a catalytic domain that is part of CE4 superfamily, all found in group I CDAs, which are known to serve critical immune function against WSSV. Finally, high expression of PmCDA1 gene in the gills of apparently healthy P. monodon was observed suggesting important basal function of the gene in this tissue. Taken together, this is a first report of the full chitin deacetylase 1 (CDA1) gene in crustaceans particularly in shrimp that exhibits putative immune function against WSSV and is distinctly highly expressed in the gills of shrimp. PMID:27335260
Full Text Available Transcriptome sequencing using Illumina RNA-seq was performed on populations of black tiger shrimp from India. Samples were collected from (i four landing centres around the east coastline (EC of India, (ii survivors of a severe WSSV infection during pond culture (SUR and (iii the Andaman Islands (AI in the Bay of Bengal. Equal quantities of purified total RNA from homogenates of hepatopancreas, muscle, nervous tissue, intestinal tract, heart, gonad, gills, pleopod and lymphoid organs were combined to create AI, EC and SUR pools for RNA sequencing. De novo transcriptome assembly resulted in 136,223 contigs (minimum size 100 base pairs, bp with a total length 61 Mb, an average length of 446 bp and an average coverage of 163× across all pools. Approximately 16% of contigs were annotated with BLAST hit information and gene ontology annotations. A total of 473,620 putative SNPs/indels were identified. An Illumina iSelect genotyping array containing 6,000 SNPs was developed and used to genotype 1024 offspring belonging to seven full-sibling families. A total of 3959 SNPs were mapped to 44 linkage groups. The linkage groups consisted of between 16-129 and 13-130 markers, of length between 139-10.8 and 109.1-10.5 cM and with intervals averaging between 1.2 and 0.9 cM for the female and male maps respectively. The female map was 28% longer than the male map (4060 and 2917 cM respectively with a 1.6 higher recombination rate observed for female compared to male meioses. This approach has substantially increased expressed sequence and DNA marker resources for tiger shrimp and is a useful resource for QTL mapping and association studies for evolutionarily and commercially important traits.
Xie, Bobo; Fu, Mingjun; Zhao, Chao; Shi, Jinxuan; Shi, Gongfang; Jiao, Zongyao; Qiu, Lihua
Macrophage migration inhibitory factor (MIF) is an ancient cytokine that engages in innate immune system of vertebrates and invertebrates. In this study, the MIF gene homologue (PmMIF) was cloned from the black tiger shrimp, Penaeus monodon. The full-length cDNA sequence of PmMIF was 838 bp and contained 78 bp 5' untranslated region (UTR) and 397 bp 3' UTR, and an open reading frame (ORF) of 363 bp which coded 120 amino acids (aa). Multiple alignment analysis showed that the deduced amino acid sequence shared 98% identities with MIF from closely related species of Litopenaeus vannamei. Quantitative real-time PCR (qRT-PCR) analysis indicated that PmMIF was highly expression observed in hepatotpancreas and gills. After Vibrio harveyi challenge, PmMIF mRNA level in hepatopancreas and gills were sharply up-regulated at 6 h post-injection, and reached the maximum at 12 h. PmMIF expression level in the hepatopancreas and gills were up-regulated markedly under low (2.3%) and high (4.3%) salinity exposure, respectively. PmMIF expression level in gills increased significantly at 12 h and reached peak values (2.5- fold, 6.4-fold and 1.8-fold compared with the control) at 12 h, 48 h and 12 h after zinc, cadmium and copper exposure, respectively. In the hepatopancreas, the expression of PmMIF reached maximum levels (8.5- fold, 6.2-fold and 2.1-fold compared with the control) at 24 h, 6 h and 48 h after zinc, cadmium and copper exposure, respectively. All the results indicate that PmMIF plays an important role in responding in the innate immune system of shrimps. PMID:27514787
Full Text Available Ten isolates of bacteria were performed from diseased black tiger shrimp collected from farms in Nakhon Si Thammarat, Patthalung, Songkhla, Satun and Patthani. All isolates belonged to Vibrio harveyi, forming rounded colonies with smooth periphery and hemolysis of red blood cells. The colonies appearedgreen and yellow on TCBS selective media and indeeded both luminescent and non-luminescent groups. To support good growth as cell proliferation, an addition of 2-8% salt to the culture media was required. Sensitivity tests against antibiotics showed that all ten isolates were sensitive to chloramphenicol, norfloxacin, trimethoprim-sulfamethoxazole, oxolinic acid, oxytetracyclin and sarafloxacin at 80, 60, 50, 40, 40 and 30%, respectively. When 12-15 g juvenile shrimp were tested for the virulence by injection, the isolates were highly virulent with OD at 640 nm in the range 0.007-0.139. The bacteria concentration of 1.60×106 - 7.27×107 CFU/ ml caused 50% juvenile mortality in ten days. The physiological changes after susceptibility included a decline in the blood cells (p<0.05, higher plasma pH (p<0.05, decrease in serum protein within 24 h (p<0.05, and a return to normal levels within 48 h. There were no differences in blood sugar levels and phenoloxidase activity between the healthy and the infected individuals. Histological study showed that at an early period of susceptibility, there were swollen tubular lumen, minor cell degeneration of the hepatic tubules and lymphoid organs and aggregation of blood cells around the degenerating cells. During 7-day susceptibility period, there was large scale cell degeneration of the hepatopancreas, lymphoid organs, gills and blood formingorgans with a great extent of blood cell aggregation and eventually mortalities occurred. During 14 day susceptibility period, there was almost entire degeneration of cells in hepatic tubules and lymphoid organs causing hepatopancreatic tubular necrosis. During this period
The molecular mechanisms of the immune system in shrimp, Penaeus monodon, are completely unknown, despite its economic importance as an aquaculture species, especially in Asia and Latin America. The genes and their gene products involved in the prophenoloxidase activating system, which is considered to be a non-self recognition and defence system in many invertebrates, have been isolated and characterised in shrimp. These include a zymogen of this cascade, prophenoloxidase (proPO); a cell adh...
P. Soundarapandian; Babu, R
In recent years, the diseases of shrimps hindered the development of shrimp culture. Hence, the use of probiotic bacteria in aquaculture has tremendous scope and the study of the application of probiotics in aquaculture has a glorious future. In the present study, the probiotics was applied (experimental) for the larval rearing of P. monodon which is compared with control tanks (without probiotics). The temperature and alkalinity of both control and experimental tanks were more of less same. ...
Suwit WUTHISUTHIMETHAVEE; Aoki, Takashi; Hirono, Ikuo; Tassanakajon, Anchalee
Availability of shrimp genome information is necessary for shrimp genetic studies and large-insert DNA clones, bacterial artificial chromosome (BACs) serve as valuable tools for obtaining genomic sequences. The construction of a BAC library was achieved from this preliminary study of P. monodon. High molecular weight (HMW) genomic DNA was isolated from abdominal muscle and the resulting hemocytes were of high quality and sufficient quantity for a BAC library construction. This BAC library was...
Full Text Available In this study, the extract of Spirulina platensis were examined in vitro to inhibit white spot syndrome virus (WSSV and application of dry S. platensis in diet for prevention of white spot syndrome (WSS in post larvae and juvenile black tiger shrimp (Penaeus monodon. The results showed that the lowest concentration of the extract for inhibiting WSSV was 0.01 mg/ml, while the optimum concentration was found to be 0.1 mg/ ml in which the mortality rate of the shrimp was 4 percents and infection was not detected from survivalshrimp by the immunohistochemistry method.Furthermore, The results showed that the survival rate of the post larvae fed on steamed egg containing dry S. platensis 5 g/kg of diet was higher than that of the control (p<0.05 when challenged with WSSV and no WSSV infected shrimp examine by polymerase chain reaction (PCR assay. In the case of juvenile shrimp, the survival rate of shrimp fed pellets containing dry S. platensis 10 g/kg of diet was higher than that of the control group (p<0.05 after challenging with WSSV. Moreover percent of WSSV infection in the survival shrimp using the immunohistochemistry method was lower than that of the control group.
Full Text Available Two feeding trial were conducted to determine the effects of various sources of carotenoid on growth performance, disease resistance, blood parameters, stress tolerance and pigmentation in juvenile black tiger shrimp (Penaeus monodon. Trial I was performed in small shrimp (1 g average body weight. The shrimp were fed with control diet without carotenoid (diet 1 while diets 2 to 6 contained 50 mg/kg astaxanthin (Lucanthin Pink®, 125 mg/kg β-carotene (Lucarotin®, 200 mg/kg β-carotene (Lucarotin®, 125 mg/kg Betatene® extracted from Dunaliella and 3% dried Spirulina respectively. There was an improvement in color in all groups of shrimp fed caroteniod supplemented diets, but no significant differences in weight gain or survival among the shrimps fed each test diet (p>0.05. Resistance to white spot syndrome virus (WSSV infection and stress tolerance (salinity stress, were not significantly different among treatments. Trial II was performed in juvenile shrimp (10 g average body weight fed test diets containing 100 ppm astaxanthin (Lucanthin pink®, 125 mg/kg β-carotene (Lucarotin®, 250 mg/kg β-carotene (Lucarotin®, 250 mg/kg Betatene® and 3% dried Spirulina compared with those fed control diet without carotenoid. At the end of 6 weeks feeding period, shrimp fed control diet as well as astaxanthin and dried Spirulina supplemented diets had higher levels of total hemocyte counts than those of all β-carotene supplemented diets feeding group. However, phenoloxidase activity and clearance of pathogenic vibrio from the hemolymphwere not significantly different among the treatments (p>0.05. Astaxanthin levels were highest in the shrimp fed all carotenoid-supplemented diets. In conclusion, a natural carotenoid i.e. dried Spirulina and carotenoid extracted from Dunaliella which have a lower production cost than analytical carotenoid showed beneficial effects on shrimp feed supplement.
Marine shrimp culture in Thailand has been developed continuously for the past two decades. This development will ensure the highest level of shrimp quality that will be suitable for the consumption of the people in the country and also aboard. The trend of culture system emphasizes on disease prevention more than treatment which will consequently limit the application of drug and chemicals. Application of pro biotic has been one means of this prevention that are commonly practiced by shrimp farmers. This research was conducted to compare the efficacy of normal Bacillus subtilis isolate from shrimp intestine and an irradiated B. subtilis as a pro biotic in shrimp feed. It was found that overall results were quite the same. These included the broth Co-culture assay. Effects on immune functions were conducted with Penaeus monodon with initial average weight of 17 gms by feeding with 3 gms/kg feed of spore of these two pro biotic for two mouths. The results indicated that both pro biotic caused significant improvement on percent phagocytosis only at the forth week of feeding trial and the overall enhancement of bactericidal activity. However, total haemocyte count and phenoloxidase activity were not altered. Total bacterial count in shrimp intestine was also conducted during the two month trial. the results indicated significant reduction of Vibrio spp. of both pro biotic groups when compared with the control. Number of Bacillus spp. in intestine were continuously high even after pro biotic treatment had been stopped Growth rate of experiment and control shrimp was not significantly different.
Full Text Available In recent years, the diseases of shrimps hindered the development of shrimp culture. Hence, the use of probiotic bacteria in aquaculture has tremendous scope and the study of the application of probiotics in aquaculture has a glorious future. In the present study, the probiotics was applied (experimental for the larval rearing of P. monodon which is compared with control tanks (without probiotics. The temperature and alkalinity of both control and experimental tanks were more of less same. The pH of the control tank w as 8.4 and the experimental tank was 8.2.The dissolved oxygen was higher in experimental tank (6.12ml/litre and lower in control tanks (5.75ml/litre. Likewise, the ammonia was higher in control tanks (0.19 mg/litre rather than experimental tanks (0.15mg/litre. The survival rate of different larval stages (nauplii, zoea and mysis were maximum in the present study than that of control tanks. The final survival rate of the post larvae from the control and experimental tank was 70 and 30% respectively. The average length of all post larvae was maximum when reared in experimental tank than control tanks. The general conclusion obtained from the present study is that the probiotics plays a vital role in maintaining water quality parameters throughout the larval cycle. It is clear from the microbial load data that vibrio sp. is dominant in the control tanks than in experimental tanks.
Full Text Available Bacillus S11 (BS11, a Gram-positive spore forming bacteria, was identified as Bacillus subtilis, based on biochemicaltests, physical morphology, and 16S rRNA gene sequence. BS11 was found to be safe as probiotic for shrimp because it doesnot produce either detectable antimicrobial substance or enterotoxin. A potential specific markers of BS11 by RAPD-PCR wasindicated using UBC459 primer (5'-GCGTCGAGGG -3' and the sequence of the major band, a size of 0.4 kb fragment, is similarto the gene encoding of a protein of the phosphotransferase system (PTS glucosamine-specific enzyme glucosamine-6-phosphate isomerase from Bacillus subtilis strain subsp. subtilis 168.
M. Abdul Kader
Full Text Available Currently, antibiotics are widely used in shrimp hatcheries to control bacterial infections. Appearance of antibiotic resistant pathogens and restriction on the use of antibiotics have led to the development of alternatives to antibiotics in hatchery systems. In light of this, an attempt was undertaken to investigate the effects of probiotics on the larval rearing of Penaeus monodon, compared with control tanks (without probiotics. The results showed that several issues significantly improved with administering probiotics in the experimental tanks compared with the tanks without probiotics. For example, the concentration of ammonia was estimated to be 1.25 mg/L that was less than half of what was measured in the control tanks. The size variation was observed more in the control tanks than in the experimental tanks. Moreover, the muscle gut ratio of PL15 was about 85 to 92% in the probiotic treated tank and 70 to 80% in the control tank during the eight cycles of production. The fouling organisms were more in the control tank compared to the experimental tanks. The average length of PL15 was maximum when reared in the experimental tanks compared to the control tanks. The final survival rate of PL15 from the control and experimental tank was 35 and 52%, respectively. The present investigation indicated that probiotics played an important role in the growth, survival and health status of P. monodon larvae.
Mundanda Muthappa Dechamma
Full Text Available The Toll-like receptors (TLR, being pattern recognition molecules, are a powerful first line of defense in response to pathogen invasion. They are known to play a crucial role in detecting and binding to the microbial molecule and triggering a non-specific immune response. Quantitative real time PCR (qPCR expression of the TLR gene was studied in healthy and Vibrio harveyi infected black tiger shrimp (Penaeus monodon. Lymphoid tissue expression of TLR in V. harveyi infected animals 24 h post injection showed statistically significant up regulation of the gene as compared to the control animals sham injected with phosphate buffered saline (PBS. The qPCR expression pattern of TLR at different time points in shrimp administered with the immunostimulant glucan for 6 days by oral feeding followed by challenge with V. harveyi showed statistically significant level at 48 h post bacterial challenge as compared to the control (immunostimulant treated animals sham injected with PBS. The novelty of the study is that it elicits the role of TLRs as important response proteins of the innate immune system in the shrimp.
Tu, Huynh Thi; Silvestre, Frederic; Wang, Neil; Thome, Jean-Pierre; Phuong, Nguyen Thanh; Kestemont, Patrick
This study examined the advantages of the use of biomarkers as an early warning system by applying it to different shrimp farming systems in Soctrang and Camau provinces, main shrimp producers in Mekong River Delta, Vietnam. Shrimp were collected at 15 different farms divided into four different farming systems: three farms were converted from originally rice paddies into intensive shrimp farming systems (IS1, IS2, IS3); three farms were rice-shrimp integrated farming systems (RS4, RS5, RS6); three farms were intensive farming systems (IS7, IS8, IS9); six farms were extensive shrimp farming systems (From ES1 to ES6). Lipid peroxidation (LPO) and total glutathione (GSH) were measured as well as catalase (CAT), glutathione peroxidase (GPX), glutathione S-transferase (GST) and acetylcholinesterase activities (ACHE). Organ specificity was observed between gills and hepatopancreas with generally higher activity of GST in gills (GSTG) whereas the contrary was observed for LPO level in gills (LPOG). Hierarchical clustering and principal component analysis clearly indicated that shrimp reared in extensive culture system formed a distinct group from those reared in intensive or rice-shrimp integrated systems. CAT in gills (CATG), GPX in gills (GPXG) and hepatopancreas (GPXHP) and ACHE in muscle (ACHEM) of shrimp collected in extensive farms showed a general higher level than those in intensively farmed shrimp. On the contrary, we observed clear high levels of GSTG and GST in hepatopancreas (GSTHP) and LPOG and hepatopancreas (LPOHP) of shrimp sampled in intensive and rice-shrimp integrated systems. Thus, we propose that LPO and CAT, GPX, GST and ACHE can be used as a set of biomarkers for the assessment of health condition and can discriminate between shrimp cultivated in different farming systems. These findings provide the usefulness of integrating a set of biomarkers to define the health status of shrimp in different shrimp culture systems. PMID:20943254
Tourtip, Somjintana; Wongtripop, Somjai; Stentiford, Grant D; Bateman, Kelly S; Sriurairatana, Siriporn; Chavadej, Jittipan; Sritunyalucksana, Kallaya; Withyachumnarnkul, Boonsirm
A new microsporidian species, Enterocytozoon hepatopenaei sp. nov., is described from the hepatopancreas of the black tiger shrimp Penaeus monodon (Crustacea: Decapoda). Different stages of the parasite are described, from early sporogonal plasmodia to mature spores in the cytoplasm of host-cells. The multinucleate sporogonal plasmodia existed in direct contact with the host-cell cytoplasm and contained numerous small blebs at the surface. Binary fission of the plasmodial nuclei occurred during early plasmodial development and numerous pre-sporoblasts were formed within the plasmodium. Electron-dense disks and precursors of the polar tubule developed in the cytoplasm of the plasmodium prior to budding of early sporoblasts from the plasmodial surface. Mature spores were oval, measuring 0.7x1.1microm and contained a single nucleus, 5-6 coils of the polar filament, a posterior vacuole, an anchoring disk attached to the polar filament, and a thick electron-dense wall. The wall was composed of a plasmalemma, an electron-lucent endospore (10nm) and an electron-dense exospore (2nm). DNA primers designed from microsporidian SSU rRNA were used to amplify an 848bp product from the parasite genome (GenBank FJ496356). The sequenced product had 84% identity to the matching region of SSU rRNA from Enterocytozoon bieneusi. Based upon ultrastructural features unique to the family Enterocytozoonidae, cytoplasmic location of the plasmodia and SSU rRNA sequence identity 16% different from E. bieneusi, the parasite was considered to be a new species, E. hepatopenaei, within the genus Enterocytozoon. PMID:19527727
Shi, Jinxuan; Fu, Mingjun; Zhao, Chao; Zhou, Falin; Yang, Qibin; Qiu, Lihua
Heat shock proteins (Hsps) are a class of highly conserved proteins produced in virtually all living organisms from bacteria to humans. Hsp60 and Hsp10, the most important mitochondrial chaperones, participate in environmental stress responses. In this study, the full-length complementary DNAs (cDNAs) of Hsp60 (PmHsp60) and Hsp10 (PmHsp10) were cloned from Penaeus monodon. Sequence analysis showed that PmHsp60 and PmHsp10 encoded polypeptides of 578 and 102 amino acids, respectively. The expression profiles of PmHsp60 and PmHsp10 were detected in the gills and hepatopancreas of the shrimps under pH challenge, osmotic stress, and heavy metal exposure, and results suggested that PmHsp60 and PmHsp10 were involved in the responses to these stimuli. ATPase and chaperone activity assay indicated that PmHsp60 could slow down protein denaturation and that Hsp60/Hsp10 may be combined to produce a chaperone complex with effective chaperone and ATPase activities. Overall, this study provides useful information to help further understand the functional mechanisms of the environmental stress responses of Hsp60 and Hsp10 in shrimp. PMID:26637414
Thi Tu, H.; SILVESTRE, F.; Wang, N; Thomé, J.-P.; Thanh Phuong, N.; Kestemont, P
This study examined the advantages of the use of biomarkers as an early warning system by applying it to different shrimp farming systems in Soctrang and Camau provinces, main shrimp producers in Mekong River Delta, Vietnam. Shrimp were collected at 15 different farms divided into four different farming systems: three farms were converted from originally rice paddies into intensive shrimp farming systems (IS1, IS2, IS3); three farms were rice–shrimp integrated farming systems (RS4, RS5, RS6);...
Paibulkichakul, C.; Piyatiratitivorakul, S.; Sorgeloos, P.; Menasveta, P.
Penaeus monodon female (49 g) and male (37 g) shrimp were fed formulated diets supplemented with 3 or 8% fish oil and 100 or 500 mg kg- 1 astaxanthin, in addition to fresh squid during a 120 day trail using a 2 × 2 × 2 factorial design. Four formulated diets were provided with different combinations of high and low concentrations of lipid (fish oil) and astaxanthin. We found that fish oil addition, at the concentrations we used did not significantly (P < 0.05) affect shrimp growth, but there ...
Full Text Available Chemical analysis of turmeric (Curcuma longa extracts using TLC/densitometry, showed an extract contain 21.57%w/w of three important curcuminoids: curcumin, desmethoxycurcumin and bisdesmethoxycurcumin. GC and MS were used to analyze volatile oils. Aromatic turmerone, α-turmerone and zingiberene were also obtained. Qualitative and quantitative analyses alcoholic extract of Andrographis paniculata using TLC, revealed that the extracts contain three important compounds in total lactone of 30.49% w/w. There are andrographolide,14-deoxy-11-12-didehydroandrographolide and neoandrographolide. TLC-chromatogram of Clinacanthus nutans extract after reacted with anisaldehyde/sulfuric acid showed a 9 key compounds, while preliminary neutralization test of the compounds revealed that there were active compounds against HSV-1 virus. In vitro efficacy test revealed that Curcuma longa and Andrographis paniculata extracts at 250 and 1,500 mg/L could eradicate 15 isolates of Vibrio spp. which were isolated from infected shrimps. Effects of medicinal plant extracts incorporated into the diet on shrimp immune responses were investigated. Shrimp fed diet containing Clinacanthus nutans extract at 20 mg/kg of diet had good growth, FCR and immune responses. The shrimp that were fed diet containing Curcuma longa extracts at 25 mg/kg of diet for 7-14 days showed high resistance to Vibrio harveyi. Likewise, the shrimp fed Andrographis paniculata extract at 25 mg/ kg of diet for 14 days had a higher resistance to WSSV. Incorporating the medicinal extracts at higher levels resulted in reduction in diet palatability which consequently had an effect on a decrease in growth, immune responses and resistance to bacterial and WSSV infection.
and combined bioassays using purified preparations of both ICE and LSNV. Despite this ambiguity, it is recommended in the interim that ICE be added to the agents such as LSNV already listed for exclusion from domesticated stocks of the black tiger shrimp.
Swollen hindgut syndrome (SHG) of tiger shrimp Penaeus monodon (Crustacea, Malacostraca, Penaeidae) post larvae: Identification of causing pathogenic bacteria and their sensitivity to some antibiotics
Sheikh Aftabuddin; Naima Akter
Swollen Hindgut syndrome (SHG) of black tiger shrimp Penaeus monodon Fabricius, 1798post larvae has been reported from Bangladesh shrimp hatcheries in recent years. At present SHG is abudding problem causing significant economic loss to the shrimp hatcheries in Bangladesh. Unlike therapid mortalities associated with viral disease such as white spot syndrome and yellow head virus,progression of SHG is gradual leading to low level mortalities without affecting swimming activity. Thesign of SHG ...
B. W. LAY
Full Text Available This study was carried out to obtain Vibrio isolates able to function as biocontr ol of vibriosis in shrimp hatchery. Thirty one Vibrio isolates were isolated from tiger shrimp larvae and hatchery environments, i.e. Labuan, Pangandaran, and Lampung, Indonesia. Pathogenic V. harveyi MR5339 was obtained from Maros, South-Sulawesi and was made as a rifampicin resistant mutant (RFR to screen for those 31 Vibrio isolates in in vitro assays and to allow us to monitor their presence in shrimp larvae and larval rearing water. Almost all Vibrio isolates could inhibit the growth of pathogenic V. harveyi MR5339 RFR. SKT-b isolate from Skeletonema was the most effective to inhibit the growth of V. harveyi MR5339 Rf* and significantly reduced larval mortality in pathogen challenge assays. These prospective biocontrol bacteria, at concentration of 10" CFU/ml, did not show pathogenicity to shrimp larvae. SKT-b was Gram negative, short rod-shape, exhibited yellow colonies on TCBS and swarming on SWC-agar media, motile, u tilized glucose and sucrose but not lactose: produced extra-cellular protease and amylase, but did not produce chitmase. Partial sequencing of 16S-rRNA gene SKT-b showed SKT-b similarity to Vibrio alginofyticus.
Full Text Available Swollen Hindgut syndrome (SHG of black tiger shrimp Penaeus monodon Fabricius, 1798post larvae has been reported from Bangladesh shrimp hatcheries in recent years. At present SHG is abudding problem causing significant economic loss to the shrimp hatcheries in Bangladesh. Unlike therapid mortalities associated with viral disease such as white spot syndrome and yellow head virus,progression of SHG is gradual leading to low level mortalities without affecting swimming activity. Thesign of SHG are a bloated or swollen in hind gut area, with the posterior digestive tract convolutingthrough the last abdominal segment. This syndrome tends to occur at later PL stages, typically afterPL10. Two types of Vibrio spp. were isolated from the swollen hindgut syndrome post larvae, identified asVibrio harveyi (Johnson and Shunk 1936and Vibrio alginolyticus (Miyamoto, Nakamura & Takizawa1961. Among these V. alginolyticus was dominant to V. harveyi. The bacterial isolates showed sensitiveto oxytetracycline (OTC, norfloxacin and ciprofloxacin and resistant to penicillin, ampicillin andamoxycillin. The luminous V. harveyi showed resistant to many antibiotics and susceptibility to only twodrugs. The cause of swollen hindgut syndrome (SHG was probably bacterial infections and poor waterquality, possibly heavy metal i.e. iron, the presence of toxic substances from chemical prophylactics andlow quality or diseased nauplii.
Selvin, J.; Lipton, A.P.
Vibriosis caused by opportunistic and secondary bacterial pathogens is still a serious disease problem in aquaculture of the black tiger shrimp Penaeus monodon. Attempts were made for controlling shrimp bacterial disease using Marine Secondary Metabolites (MSMs). Findings indicated that the MSMs of seaweed Ulva fasciata and Dendrilla nigra are effective for controlling shrimp bacterial pathogens.
The effect of gamma irradiation (0, 1, 2, 4 and kGy) and frozen storage (-100C) of 6 months on the microbiological, chemical and organoleptic qualities of whole (WSI) and peeled (PS2) tiger shrimps (Peneaus monodon) were studied. The qualities of the products were compared with blanched shrimps (PSI) ordinarily prepared at a factory. The irradiation dose up to 6 kGy reduced the total bacterial count (TBC) by at least 3 log cycles with D value = 2.13 kGy. The survival of pathogenic microbes was effectively retarded. Although blanching eliminated the pathogenic microbes, the TBC of PSI before storage is still in a range of 105/g. There was an increase in TBC of 1-2 log cycles during storage in all samples. Peeling effectively reduced the TBC of the shrimps. Gamma irradiation and shorter period of storage time did not influence the pH and protein contents of the products. Indole production was slowed down by irradiation at 1-4 kGy, which is comparable to blanching treatment. Organoleptic qualities (colour, ordour and texture) of the products were not effected by irradiation and storage time except for samples irradiated at 6 kGy in which the pink colour faded. There was no significant difference in the organoleptic qualities of irradiated (2 kGy) samples as compared to blanched products. (author)
Chaivisuthangkura, Parin; Longyant, Siwaporn; Sithigorngul, Paisarn
Among shrimp viral pathogens, white spot syndrome virus (WSSV) and yellow head virus (YHV) are the most lethal agents, causing serious problems for both the whiteleg shrimp, Penaeus (Litopenaeus) vannamei, and the black tiger shrimp, Penaeus (Penaeus) monodon. Another important virus that infects P. vannamei is infectious myonecrosis virus (IMNV), which induces the white discoloration of affected muscle. In the cases of taura syndrome virus and Penaeus stylirostris densovirus (PstDNV; formerl...
Fuller, Pam L.; Knott, David M.; Kingsley-Smith, Peter R.; Morris, James A.; Buckel, Christine A.; Hunter, Margaret E.; Hartman, Leslie D.
After going unreported in the northwestern Atlantic Ocean for 18 years (1988 to 2006), the Asian tiger shrimp, Penaeus monodon, has recently reappeared in the South Atlantic Bight and, for the first time ever, in the Gulf of Mexico. Potential vectors and sources of this recent invader include: 1) discharged ballast water from its native range in Asia or other areas where it has become established; 2) transport of larvae from established non-native populations in the Caribbean or South America via ocean currents; or 3) escape and subsequent migration from active aquaculture facilities in the western Atlantic. This paper documents recent collections of P. monodon from the South Atlantic Bight and the Gulf of Mexico, reporting demographic and preliminary phylogenetic information for specimens collected between North Carolina and Texas from 2006 through 2012. The increased number of reports in 2011 and 2012, ranging from 102 mm to 298 mm total length, indicates that an adult population is present in densities sufficient for breeding, which is indicative of incipient establishment. Based on these reports of P. monodon, its successful invasion elsewhere, and its life history, we believe that this species will become common in the South Atlantic Bight and Gulf of Mexico in less than 10 years. Penaeus monodon is an aggressive predator in its native range and, if established, may prey on native shrimps, crabs, and bivalves. The impacts of an established P. monodon population are potentially widespread (e.g., alterations in local commercial fisheries, direct and indirect pressures on native shrimp, crab and bivalve populations, and subsequent impacts on the populations of other predators of those organisms) and should be considered by resource managers. The impacts of P. monodon on native fauna and the source(s) or vector(s) of the invasion, however, remain unknown at this time.
Full Text Available In the present study, an attempt has been made to culture the giant tiger shrimp, P. monodon in six ponds each with 0.8 ha near Alagankulam village of Ramanathapuram district, Tamil Nadu. The salinity of the six ponds was ranging between 16-30 ppt and pH was 7.6 to 8.0. Minimum 3.9 ppm dissolved oxygen and maximum 4.2 ppm was recorded during the culture period. The temperature was ranging between 27 to 30C and the transparency was 35 to 50 cm. Harvesting was carried out in ponds 1, 2 at DOC 142 and 3, 4 at DOC 143 and 5,6 at DOC 149. Average body weights of the ponds 1, 2, 3, 4, 5 and 6 are 38.46, 40.00, 40.00, 37.00, 38.46 and 37.00, respectively. Highest survival 76% was recorded in pond 4 and the lowest survival 68% recorded in pond 1. The average survival for all six ponds was calculated as 72%. Maximum production was reported in pond 2 (2,154 kg and minimum was in the pond 1 (1,874 kg. The total production in all ponds was 11,998 tons. The average body weight and average daily growth was calculated as 38.49 and 0.26 g, respectively in all ponds. The maximum amount of feed was consumed by the shrimps in pond 2 (2,940 and minimum was in pond 1 (2,550. The FCR for all ponds were 1.36. Maximum net profit was obtained from pond 2 (Rs. 3,91,940 and minimum was in the pond 1 (Rs. 2, 69,492. So it is confirmed that 12 m-2 is an ideal stocking density for the culture of P. monodon as evidenced from the net profit Rs. 19,18,710. To get this profit, proper water quality management and feed management is essential.
Mohammad M. Monwar
Full Text Available Jumbo tiger prawn, Penaeus monodon is the world’s most popular cultivable species for itsfast growth, hardy nature, delicious taste and market demand. During the last three decades shrimpculture has been expanded rapidly. White spot Syndrome Virus (WSSV is a great treat to this cultureexpansion. In this research, WSSV free fries were stocked in four coastal ponds with different salinities.The stocking density was kept low and constant. Water parameters, fish growth, and WSSVcontamination were recorded fortnightly. The WSSV contamination was primarily detected by using EnbioShrimp Virus Detection Test Kit, ‘Shrimple’ and further confirmed by the PCR test. Among the ponds,Pond A, B, and C were in completely controlled environmental condition where as pond D was traditionalone that exposed to tidal variations. Physico-chemical parameter varied from as, temperature 29 to32ºC, salinity 0 to 31‰, water pH 7.1 to 8.3, dissolved oxygen 3.8 to 6.3 mL L-1, alkalinity 80-122 mg L-1,ammonia 0 to 1.5 mg L-1 and transparency 23 to 50 cm. The WSSV was found positive in the pond D justafter hundred days, when the average weight of the shrimps was 26.69 g. It is observed that rapidchange in the salinity and temperature, poor environmental conditions and uncontrolled exchange ofwater made the shrimp more vulnerable to the WSSV.
Two lots of frozen precooked shrimps from Thailand ''PENAEUS MONODON'' Black tiger variety were irradiated at 1.8 to 3.6 kGy. This way, it was hoped to compare the effects of gamma irradiation on the microbiological quality and the organoleptic properties of frozen precooked shrimps after transportation from Thailand to Canada. The results indicated that the extension of shelf-life based on mesophiles content was from 33 days for the control to more than 47 days for the irradiated shrimps stored at 3 ± 1oC. The results of sensory evaluation gave slightly fresher odor for the control than the irradiated shrimps. On day one, this effect was more apparent. The results of hedonic tests showed that the irradiated shrimps were acceptable during storage. In conclusion, the results demonstrate that the combined treatments (freezing plus irradiation) of precooked shrimps are useful for increasing the storage life of shrimps without affecting consumer acceptability. (Author)
Chiou, Tzu-Ting; Lu, Jenn-Kan; Wu, Jen-Leih; Chen, Thomas T; Ko, Chi-Fong; Chen, Jiann-Chu
A penaeidin family, mo-penaeidin was cloned from the haemocytes of tiger shrimp Penaeus monodon using genomic polymerase chain reaction (PCR) by gene specific primers. Analysis of nucleotide sequence revealed that this mo-penaeidin consists of 1348 bp containing one intron (680 bp) and two exons (210 and 458 bp). It has an open reading frame (ORF) of 222 p, which encodes a protein of 74 amino acids including a signal peptide of 19 amino acids. The calculated molecular mass of the mature protein (55 amino acids) is 6.059 kDa with an estimated pI of 9.3. The deduced amino acid sequence of mo-penaeidin has similarity to that of penaeidin from Fenneropenaeus chinensis (73%), Farfantepenaeus paulensis (66%), Litopenaeus schmitti (53-67%), L. stylirostris (50-67%), L. setiferus (50-62%), L. vannamei (44-66%), and Marsupenaeus japonicus (33%), respectively. Phylogenetic tree analysis indicated that penaeidin (including mo-penaeidin, penaeidin, and penaeidin 5, 2, 3k, 3c1) of P. monodon is distinct from penaeidin 1, penaeidin 2, penaeidin 3 and penaeidin 4 of other penaeid shrimps. The mo-penaeidin mRNA was detected in various tissues including ovary and mandibular organ. The mo-penaeidin mRNA was present in one cell to postlarva stage with higher level at nauplius I (9h post hatching) and higher expression during the intermoult stage indicating an early innate immunity and different immunity at moulting stage. PMID:16820207
Christian Reinhard Vogl
Full Text Available In Bangladesh, black tiger shrimp (Penaeus monodon; Fabricius, 1798 aquaculture has come to be one of the most important sectors in both the rural and national economies. Likewise, organic shrimp aquaculture has emerged as an alternative farming enterprise for farmers especially in the southwestern districts of Bangladesh. The present study aims to show key performance characteristics of organic shrimp farmers and farming in a prototypical shrimp farming area in Bangladesh. Data was collected in 2009 from organic shrimp farmers in the Kaligonj and Shyamnagar sub-districts through questionnaire interviews, transect walks and focus group discussions. The mean productivity of organic shrimp farming in the area is 320 kg ha−1 yr−1 (ranging from 120 to 711 kg ha−1year−1. Organic farmers are more likely to have a higher monthly income and less aquaculture experience. Moreover, suitable landholdings and classified labor distribution have been found to play an important role in the development of organic shrimp aquaculture. The most common assets of organic shrimp aquaculture are high yield, low production cost, available post larvae and high market prices. Small business farmers are likely to earn more income benefits from organic shrimp aquaculture than their larger-scale counterparts. Finally, the paper suggests that more research is needed to stimulate the success of organic shrimp aquaculture.
Two lots of frozen precooked shrimps from Thailand ''PENAEUS MONODON'' Black tiger variety were irradiated at 1.8 to 3.6 kGy. This way, it was hoped to compare the effects of gamma irradiation on the chemical and physical qualities of frozen precooked shrimps after transportation from Thailand to Canada. The results indicated that the color measurement expressed in terms of lightness (L*) showed a relative stability during storage of processed shrimps. However, based on the value (θ) a more intense red color was observed for the control and the reference. The cooked irradiated and nonirradiated shrimps presented a significant decrease (p ≤ 0.01) of 22% in firmness during the first week of storage. Thus, irradiation did not impair the firmness of shrimps after treatment or during storage. Also, based on the statistical analysis, the irradiation treatment did not affect the content of amino acids of precooked shrimps. (Author)
Meidam, J; Broeck, C Van Den; Veitch, J; Sathyaprakash, B S
The Einstein Telescope (ET), a proposed third-generation gravitational wave observatory, would enable tests of the no-hair theorem by looking at the characteristic frequencies and damping times of black hole ringdown signals. In previous work it was shown that with a single $500 - 1000\\,M_\\odot$ black hole at distance $\\lesssim 6$ Gpc (or redshift $z \\lesssim 1$), deviations of a few percent in the frequencies and damping times of dominant and sub-dominant modes would be within the range of detectability. Given that such sources may be relatively rare, it is of interest to see how well the no-hair theorem can be tested with events at much larger distances and with smaller signal-to-noise ratios, thus accessing a far bigger volume of space and a larger number of sources. We employ a model selection scheme called TIGER (Test Infrastructure for GEneral Relativity), which was originally developed to test general relativity with weak binary coalescence signals that will be seen in second-generation detectors such ...
Full Text Available Shrimp hatcheries often beset with diseases, mainly the bacterial infection and antibiotics arewidely used for prevention of disease. Presently, beneficial bacteria (probiotics are used to preventdiseases instead of antibiotics and increasing the production. In the present study, the two new microbialstrains Bacillus megaterium and Streptomyces fradiae isolated from mangrove sediments were applied(experimental culture for the post larval rearing of Penaeus monodon which is compared with controlculture tanks (without probiotics. The water quality condition such as temperature (27-29oC, salinity(26-28‰ and dissolved oxygen (4.7-5.0 mg L-1 of both control and experimental culture were more orless similar. Concentration of ammonia and pH were significantly different (p<0.05 between the controland experimental culture during the study period. The feed assimilation efficiency is higher (above 80%in experimental culture tank when compared to control tank (74.76%. The growth rate was higher -1.70 and 1.67 in S. fradiae, 1.66 and 1.63 in B. megaterium - through feed and water, respectively,while in control tank it was 1.4. The FCR values were 2.06 and 2.12 in S. fradiae treated tanks throughfeed and water, respectively, while 2.51 and 2.55 were observed in B. megaterium treated through feedand water respectively. The FCR value was found higher (4.02 in the control tank. The average totalheterotrophic bacteria (THB and total presumptive Vibrio bacteria both in culture water and post larvaewere lower during experimental culture in compared to control culture. The present study indicates thatthe probiotic treatment using two new microbial strains such as B. megaterium and S. fradiae would helpin better aquaculture production.
Full Text Available The tiger shrimp (Penaeus monodon is an Indo-Pacific species. Its global production between 1970 and 1980 exceeded all other shrimp species, which favored its introduction and cultivation outside its natural range in several countries of Africa, Europe, USA and South America. It is currently found in the coast of the Atlantic Ocean (Mexico, United States, Puerto Rico, Brazil, Guyana, Venezuela and Colombia. Despite the risk involved, no studies have been conducted to evaluate their impact as a possible invasive species and their genetic condition. This study evaluated the genetic status and population origin of P. monodon in the northernmost Colombian Caribbean, analyzing the mitochondrial DNA control region (mtDNA-CR. 16 individuals were randomly collected from Golfo de Salamanca and 342 original Indo-Pacific sequences were obtained from GenBank. Parameters of genetic diversity and genetic relationships were analyzed. There were a total of 358 sequences compared and 303 haplotypes identified. Three haplotypes were identified in the Colombian population. This results showed lower genetic diversity compared with Indo-Pacific populations. These haplotypes were closely related to those found in samples from the Philippines and Taiwan. We discuss the need to create a regional network to characterize the established populations in the Great Caribbean, with the purpose of inferring colonization processes and the establishment of management measures.Estado genético, origen y riesgo de establecimiento del camarón tigre gigante (Penaeidae: Penaeus monodon, una especie inavasora en aguas del Caribe colombianoEl camarón tigre (Penaeus monodon es una especie del Indo-Pacífico. Su producción mundial entre 1970 y 1980 superó todas las otras especies de camarón, lo que favoreció su introducción y cultivo fuera del área de distribución natural en varios países de África, Europa, EE.UU. y América del Sur. Actualmente se encuentra en la costa del
Full Text Available A survey on 58 shrimp farms (42 operational and 16 non-operational was conducted within the Manroe island over 12 weeks. The black tiger shrimp, Penaeus monodon was the main species cultured. Presently, small-scale farmers carry out 90% shrimp aquaculture in Manroe Island. The farming is characterized by modified extensive and semi-intensive production systems, largely dependent on small ponds of less than 1 ha. These farms account for ~90% of the total area utilized for shrimp culture. The stocking densities range between 4 to 6 post larvae /m2 and average shrimp yields after 90-120 days is 320-400 kg.ha-1. As per our survey the average production cost for shrimp per hectare is at least 1,50,000 per cycle. During the 1990s, shrimp was cash crop in the Kollam with an average production of 900 kg.ha-1. Currently, the unsustainable practices and devastating outbreaks of disease has forced the industry to shut down.
Chaivisuthangkura, Parin; Longyant, Siwaporn; Sithigorngul, Paisarn
Among shrimp viral pathogens, white spot syndrome virus (WSSV) and yellow head virus (YHV) are the most lethal agents, causing serious problems for both the whiteleg shrimp, Penaeus (Litopenaeus) vannamei, and the black tiger shrimp, Penaeus (Penaeus) monodon. Another important virus that infects P. vannamei is infectious myonecrosis virus (IMNV), which induces the white discoloration of affected muscle. In the cases of taura syndrome virus and Penaeus stylirostris densovirus (PstDNV; formerly known as infectious hypodermal and hematopoietic necrosis virus), their impacts were greatly diminished after the introduction of tolerant stocks of P. vannamei. Less important viruses are Penaeus monodon densovirus (PmDNV; formerly called hepatopancreatic parvovirus), and Penaeus monodon nucleopolyhedrovirus (PemoNPV; previously called monodon baculovirus). For freshwater prawn, Macrobrachium rosenbergii nodavirus and extra small virus are considered important viral pathogens. Monoclonal antibodies (MAbs) specific to the shrimp viruses described above have been generated and used as an alternative tool in various immunoassays such as enzyme-linked immunosorbent assay, dot blotting, Western blotting and immunohistochemistry. Some of these MAbs were further developed into immunochromatographic strip tests for the detection of WSSV, YHV, IMNV and PemoNPV and into a dual strip test for the simultaneous detection of WSSV/YHV. The strip test has the advantages of speed, as the result can be obtained within 15 min, and simplicity, as laboratory equipment and specialized skills are not required. Therefore, strip tests can be used by shrimp farmers for the pond-side monitoring of viral infection. PMID:24567913
Patil, R.; Palaksha, K.J.; Anil, T.M.; Guruchannabasavanna; Patil, P.; Shankar, K.M.; Mohan, C.V.; Sreepada, R.A.
, of Karnataka on the west coast of India. Of 12 grow-out farms in Kundapur, 6 (F1 to F6) yielded shrimp samples that were negative for WSSV by both immunodot test and 1-step PCR from stocking to successful harvest. Samples from the other 6 farms (F7 to F12) were...
NATIVE only to China and Russia’s Siberia, the Dongbei (Northeast) Tiger is a magnificent wild animal. Over the last few decades, the sound of its roar has become weaker and weaker. In the 1970s, over 80 wild Dongbei Tigers still roamed the northeast of China. By the 1980s, though, it had become very difficult to track these tigers. To obtain a comprehensive
Full Text Available This research was carri ed out to st udy genetic di versity of ampici llin-resi sta nt Vi brio from variou s stages o f t iger shrimp larvae (Penaeus Monodon de velopment from,Ta mbak Inti Raky at hatchery, near Labuan, West Jav a, Indonesia. A total of 25 am picillin- resistant Vi brio is ol at es were isolated using thiosul pha te citrat e bi le -sal t sucr os e agar ( TCBS -Aga r a nd s eawa ter complet e agar (SWC-Agar. Physiological and bioc he mical cha racteri zation showed that the i solates could be grouped into only two species, i.e. V. har veyi from the egg s tage; and V. metschnikovii from larvae and post-larval stage (i.e nauplius, zoe a, mysis, PLi, P L5, PL,0, and P L,5. Thes e is olates were also present i n t heir r espective rearing wate r of each s tage and som e natural feed. Schi zoty ping analysis e mployin g rest riction endonuclease Noll (5 '-GC4GGCCGC indicate d that the is ol at es could be grouped i nto at l east 13 differ en t ge no ty pe s. Therefore, schizoty ping was more di sc ri mi native than phy siologica l charac te riz ati on . This stud y showed that particu lar grou ps of Vi brio colonize d all s tages of shrimp la rvae and de monstrated cl os ed ph yl og en et ic r elationsh ip. These gro ups of Vi brio mi gh t be the do mi na nt microbiota whic h could suppress the de velopment of othe r Vi brio in cl ud ing th e pathogen ic Vi brio.
GUO Zhixun(郭志勋); Karin van de Braak; Magriet Botterbloom
Distribution of injected Vibrio anguillarum in body of Penaeus monodon was studied with immunohistochemical method. Bacteria could be detected throughout the experiment in some individuals; however in lymphoid tissue, gill, heart and haemolymph of all vibrio injected shrimp, the bacteria could be observed only 5 min after injection. The bacteria density in haemolymph, haemolymph of the hepatopancreas and gills decreased with time. In the lymphoid organ and heart, the bacteria density was the highest 48 h after injection, then decreased. Nodules could be formed in the heart, lymphoid organ and injection site.
Genetic drift is a concept of population genetics that is central to understanding evolutionary processes and aspects of conservation biology. It is frequently taught using rather abstract representations. I introduce three real-life zoological examples, based on historical and recent color morphs of tigers, tapirs, and ravens, that can complement…
Kansas Data Access and Support Center — The 2010 Census TIGER/Line data was merged together into a statewide layer if originally available by county from the US Census Bureau. The spatial data was then...
Kansas Data Access and Support Center — As suggested by the GIS Technical Advisory Committees DASC subcommittee, the DASC staff has imported and converted the U.S. Bureau of Census 2000 TIGER Line files...
Kansas Data Access and Support Center — As suggested by the GIS Technical Advisory Committees DASC subcommittee, the DASC staff has imported and converted the U.S. Bureau of Census 1992 Tiger Line Files...
Sabri Bilgin; Necla Ylmaz
Objective:To determine growth and reproduction biology ofPhilocheras trispinosus (P. trispinosus) with abundance of other caridean shrimps caught by beam trawl in the Southern Black Sea. Methods: Samplings were carried out monthly with a beam trawl of 2 m length and 15 mm cod-end mesh size between December 2012 and November 2013. All individuals were sampled between 1 and 40+ m water depth from 146 hauls. Results:A total of 550P. trispinosus, 618Palaemon adspersus, 12Palaemon serratus, 11Philocheras fasciatus and 10Crangon crangon were sampled during the study period. The crangonid shrimps started appearing during the winter and spring and the number of individuals increased to reach their highest value in mid-winter and mid-spring. The seasonal von Bertalanffy growth parameters forP. trispinosus were estimated asL∞ = 29.3 mm total length,K = 0.860/year,t0 = –0.900 year,C = 0.180, andts= 0.010. The start of the slow growth period was at the beginning of July (WP = 0.510). Ovigerous females appeared in the sampling area between January and April. Size at sexual maturity was estimated as 25.69 mm total length. Conclusions: The results supported the distribution and abundance of caridean shrimp species from the study area and the population dynamics of the most abundant shrimp species,P. trispinosus. The current study can be answered as baseline data prior to management strategies to ensure sustainable conservation of the shrimp species.
Shrimp account for 15 percent of the total value of internationally traded fishery products, and currently are the largest single aquaculture commodity in value terms. The Pacific whiteleg shrimp (Litopenaeus vannamei) generated USD 11 billion from a production volume of 2.7 million metric tons (mm...
Yale law professor Amy Chua has created waves in the media over her "Chinese" parenting style,which she documented in her memoir Battle Hymn of the Tiger Mother. Much has been made of her assertion that an A- is a bad grade and her reveal that she called one of her children "garbage." Yet her eldest daughter performed at Carnegie Hall
Wilting, Andreas; Courtiol, Alexandre; Christiansen, Per;
approaches to plan tiger recovery are partly impeded by the lack of a consensus on the number of tiger subspecies or management units, because a comprehensive analysis of tiger variation is lacking. We analyzed variation among all nine putative tiger subspecies, using extensive data sets of several traits...
Gudkovs, Nicholas; Murwantoko, Murwantoko; Walker, Peter J
The white spot syndrome virus (WSSV) genome contains 3 variable number tandem repeat (VNTR) regions, located in open reading frame (ORF) 75, ORF94 and ORF125, which have been employed for molecular epizootiological studies. A previous report suggested that the ORF 94 VNTR is highly unstable, varying in the number of tandem repeats during single passages from shrimp to other crustaceans. As such rapid variations would have profound implications for the interpretation of molecular epizootiological data, we re-examined the stability of the ORF94 VNTR. Two WSSV isolates with different ORF94 VNTR genotypes (TRS5 and TRS7) were obtained from disease outbreaks in farmed black tiger shrimp Penaeus monodon in Indonesia. High titre stocks of each virus were produced by injection in specific pathogen-free (SPF) Pacific white shrimp Litopenaeus vannamei with filtered infected tissue extracts, and the genotypes were confirmed. Each stock (macerated tissue) was then used to feed SPF Pacific white shrimp, freshwater crayfish (Cherax sp.) and freshwater prawns Macrobrachium rosenbergii through 3 successive passages involving alternative hosts at each level. Taqman real-time PCR was conducted on samples from each group to confirm infection and quantify viral genetic loads. ORF94 VNTR genotype analysis conducted on samples from each of the 43 passage groups indicated no variations in the VNTR number in either genotype TRS5 or genotype TRS7. This finding is contrary to the previous report and suggests that ORF94 VNTR are stable during multiple passages in these crustaceans. PMID:25320037
Tigers are facing extinction and urgently need conservation This year in the Chinese lunar calendar is the Year of the Tiger, ever regarded as the most regal of animals. But today they are being pushed to the brink of extinction.
This paper will address the purpose, scope, and approach of the Department of Energy Tiger Team Assessments. It will use the Tiger Team Assessment experience of Sandia National Laboratories at Albuquerque, New Mexico, as illustration
Klowkliang, T.; Tattanon, T.; Ruangsri, J.; Supamattaya, K.; Suntornchareonnon, N.; Boonyaratpalin, M.
Chemical analysis of turmeric (Curcuma longa) extracts using TLC/densitometry, showed an extract contain 21.57%w/w of three important curcuminoids: curcumin, desmethoxycurcumin and bisdesmethoxycurcumin. GC and MS were used to analyze volatile oils. Aromatic turmerone, α-turmerone and zingiberene were also obtained. Qualitative and quantitative analyses alcoholic extract of Andrographis paniculata using TLC, revealed that the extracts contain three important compounds in total lactone of 30.4...
Prevalence of Taura syndrome virus (TSV and Infectious hypodermal and haematopoietic necrosis virus (IHHNV in white shrimp (Penaeus vannamei populations and susceptibility to infection of some aquatic species native to Thailand
Full Text Available This study aimed to survey the prevalence of some infectious diseases e.g. Taura syndrome virus (TSV and Infectious hypodermal and haematopoietic necrosis virus (IHHNV in white shrimp (Penaeus vannamei populations and to assess the impact of such infectious agents to indigenous aquatic animals in Thailand. Samples of both larval and juvenile or adult shrimp from each region of the country were collected and screened for TSV and IHHNV using the polymerase chain reaction (PCR technique. Viruses isolated from affected shrimp were used for determine the susceptibility to infection of some aquatic species native to Thailand.A total of 163 samples of larval shrimp from hatcheries were screened. The results showed infection with TSV and IHHNV in 3.68 and 44.17%, respectively. As high as 7.32% TSV infection was detected in shrimp samples collected from the South Eastern coast, followed by the Eastern and Central regions with percentages of 5.56 and 4.53, respectively. Shrimp with the highest rate of IHHNV infection, 55.56% were collected from the Eastern region. A total of 192 samples of shrimp reared in grow-out ponds were also collected. The results showed shrimp were infected with TSV and IHHNV with percentages of 6.67 and 67.19, respectively. The highest prevalence of IHHNV (up to 90% was found in samples collected from the lower Southern region. The highest prevalence of TSV infection (11.29% was reported in shrimp from the Central region. A study of the susceptibility to TSV and IHHNV infection of some indigenous aquatic species of Thailand was also carried out. The results showed many aquatic species native to Thailand e.g. black tiger shrimp (Penaeus monodon, speckled shrimp (Metapenaeus monoceros, dwarf prawn (Macrobrachium equideus, krill (Acetes sp., mantis lobster (Chloridopsis immaculatus, freshwater prawn (Macrobrachium lanchesteri and M. rosenbergii, mangrove crab (Sesarma sp. and mud crab (Scylla serrata were susceptible to viruses and
Peregrino-Uriarte, Alma B; Varela-Romero, Alejandro; Muhlia-Almazán, Adriana; Anduro-Corona, Iván; Vega-Heredia, Sarahí; Gutiérrez-Millán, Luis E; De la Rosa-Vélez, Jorge; Yepiz-Plascencia, Gloria
Mitochondria play key roles in many cellular processes. Description of penaeid shrimp genes, including mitochondrial genomes are fairly recent and some are focusing on commercially important shrimp as the Pacific shrimp Litopenaeus vannamei that is being used for aquaculture not only in America, but also in Asia. Much less is known about other Pacific shrimp such as the yellowleg shrimp Farfantepenaeus californiensis and the blue shrimp Litopenaeus stylirostris. We report the complete mitogenomes from these last two Pacific shrimp species. Long DNA fragments were obtained by PCR and then used to get internal fragments for sequencing. The complete F. californiensis and L. stylirostris mtDNAs are 15,975 and 15,988 bp long, containing the 37 common sequences and a control region of 990 and 999 bp, respectively. The gene order is identical to that of the tiger shrimp Penaeus monodon. Secondary structures for the 22 tRNAs are proposed and phylogenetic relationships for selected complete crustacean mitogenomes are included. Phylogenomic relationships among five shrimp show strong statistical support for the monophyly of the genus across the analysis. Litopenaeus species define a clade, with close relationship to Farfantepenaeus, and both clade with the sister group of Penaeus and Fenneropenaeus. PMID:20403743
Presents an art lesson used with sixth-grade students in which they painted their school mascot (a tiger) in the style of a famous artist. Explains that students selected an artist, such as Andrew Wyeth or Edvard Munch. Describes how the students created their tigers. (CMK)
@@ Though this year is the year of Tiger according to Chinese lunar calendar, local animal protection officials in northeast China's Liaoning Province have revealed that a total of 11 Siberian tigers have died in the Shenyang Forestry Wild Animals Zoo (SFWAZ) since November 2009.
Earth Data Analysis Center, University of New Mexico — TIGER, TIGER/Line, and Census TIGER are registered trademarks of the Bureau of the Census. The Redistricting Census 2000 TIGER/Line files are an extract of selected...
Earth Data Analysis Center, University of New Mexico — TIGER, TIGER/Line, and Census TIGER are registered trademarks of the Bureau of the Census. The Redistricting Census 2000 TIGER/Line files are an extract of selected...
Earth Data Analysis Center, University of New Mexico — TIGER, TIGER/Line, and Census TIGER are registered trademarks of the Bureau of the Census. The Redistricting Census 2000 TIGER/Line files are an extract of selected...
Earth Data Analysis Center, University of New Mexico — This data set is a vector polygon digital data structure taken from Census Bureau's TIGER/Line Files, 1994, for New Mexico. The source software used was ARC/INFO...
According to Chinese Lunar Canlendar 1998 is the Year of the Tiger. In the end of 1997, people began to talk about the coming year. Everything about tiger was already becoming popular. The public propaganda Organs such as IV states, radio stations and newspapers were the first to guide the public’s attentions to "Tigers". It was no longer a strange
陈弘成; 吴雅琪; 陈富美; 吴瑞梹
South East Asia is the center of farmed shrimp, and China tops the shrimp production. Cultured shrimp species has changed gradually in South East Asia, and white shrimp becomes the most important farmed shrimp species in the world. Production of white shrimp reached 76％ of the total farmed production in 2006, while it was only 62％ in 2004. However,production of black tiger shrimp has increased in India, Indonesia and Bangladesh, for their bigger size harvested in extensive culture system. In the USA and Japan, the supply of shrimp surpassed the demand, resulting in gradually decreased market price. There is no way to stabilize the price unless bigger size shrimp produced at right seasons is encouraged as in Vietnam. In super intensive white shrimp culture system, production of over 40t.hm-2 per crop can be achieved. White shrimp can tolerate very low salinity water, and many freshwater areas have been converted into shrimp ponds. Finally, some suggestions for shrimp culture development are put forward in this paper.%东南亚是世界虾类养殖的重心地区,而白虾已成为世界养虾中最重要的虾种.中国为世界养虾产量最多的地区.在东南亚的养虾中,白虾的产量占总产量的比例从2004年的62%增加到2006年的76%,而草虾产量占总产量的比例则从38%减少到24%.但是印度、印度尼西亚与孟加拉国等地区的草虾产量不减反增,其中以印度为最,这与当地低密度的放养方式生产的较大体形的草虾有关.美国和日本的养虾产量由于供过于求,故虾价仍缓慢下跌,若养殖业者能把握虾价较高的季节(9月至次年1月)并生产大型虾类,则养殖利润仍高,越南的草虾即为一例.文中还提及白虾超高密度养殖的成功方法,许多地区的每季白虾生产可达40t·hm以上;淡水区白虾养殖可不必加以禁养,因其对环境的危害影响仍不明显.本文最后还提出发展虾类养殖的科研重点,以供有识者参考.
Mukhacheva, Anna S; Derugina, Vasilissa V; Maksimova, Galina D; Soutyrina, Svetlana V
Anthropogenic impacts are the primary threats to Amur tigers (Panthera tigris altaica) and their habitat. Villagers living in proximity to tigers tend to view them negatively and, often, as a source of revenue on black markets. We aim to reduce human-tiger conflict by working with young students of Ternei County in the heart of tiger habitat in Primorskii Krai (Province). To inform and influence Ternei County's future decision-makers, we developed "Safe Conduct", a year-long education program held in 6 villages, culminating in a multi-school conference. We tested the efficacy of Safe Conduct as a potential model for tiger conservation educational programs. We measured levels of student knowledge about tiger ecology, their attitude towards tigers, and their willingness to engage in tiger conservation activites prior to, immediately after and 6 months following the completion of our program. Results supported the fundamental premise of Safe Conduct that knowledge and attitude towards tigers are correlated. Knowledge of tiger ecology and attitude towards tigers increased by the project's completion; both remained high after 6 months. However, commitment to participation in conservation efforts rose temporarily post-program and then dropped. Results varied by village. We recommend that the reasons for the high performance measures of students in 2 villages be investigated, and that lessons learned be applied to villages that underperformed. Safe Conduct represents a potential model for environmental education programs in Ternei County and elsewhere to educate future generations, to eventually develop a strong commitment to Amur tiger conservation at the community level. PMID:26096458
Tangprasittipap, Amornrat; Srisala, Jiraporn; Chouwdee, Saisunee; Somboon, Montagan; Chuchird, Niti; Limsuwan, Chalor; Srisuvan, Thinnarat; Flegel, Timothy W; Sritunyalucksana, Kallaya
Background The microsporidian Enterocytozoon hepatopenaei was first described from Thailand in 2009 in farmed, indigenous giant tiger shrimp Penaeus (Penaeus) monodon. The natural reservoir for the parasite is still unknown. More recently, a microsporidian closely resembling it in morphology and tissue preference was found in Thai-farmed, exotic, whiteleg shrimp Penaeus (Litopenaeus) vannamei exhibiting white feces syndrome (WFS). Our objective was to compare the newly found pathogen with E. ...
National Oceanic and Atmospheric Administration, Department of Commerce — The Northern Shrimp Survey was initiated in 1983 by the Atlantic States Marine Fisheries Commission (ASMFC) and monitors the relative abundance (number of shrimp),...
Steich, D J; Brugger, S T; Kallman, J S; White, D A
This final report describes our efforts on the Three-Dimensional Massively Parallel CEM Technologies LDRD project (97-ERD-009). Significant need exists for more advanced time domain computational electromagnetics modeling. Bookkeeping details and modifying inflexible software constitute a vast majority of the effort required to address such needs. The required effort escalates rapidly as problem complexity increases. For example, hybrid meshes requiring hybrid numerics on massively parallel platforms (MPPs). This project attempts to alleviate the above limitations by investigating flexible abstractions for these numerical algorithms on MPPs using object-oriented methods, providing a programming environment insulating physics from bookkeeping. The three major design iterations during the project, known as TIGER-I to TIGER-III, are discussed. Each version of TIGER is briefly discussed along with lessons learned during the development and implementation. An Application Programming Interface (API) of the object-oriented interface for Tiger-III is included in three appendices. The three appendices contain the Utilities, Entity-Attribute, and Mesh libraries developed during the project. The API libraries represent a snapshot of our latest attempt at insulated the physics from the bookkeeping.
Chinese parents face dilemmas in choosing education methods for children Meng Yijun,an accountant at a PR firm in Beijing,has a 10-yearold daughter. Although she was born in 1974,the Year of the Tiger according to Chinese astrology,Meng said
The literature was reviewed for information on the chemical aspects of irradiated shrimp. Low-dose irradiation can effectively control spoilage and pathogenic organisms and extend the refrigerated shelf life of shrimp. Radiation-induced increases in black discoloration occur in some species during storage, but can be minimized by proper blanching. Some loss of the characteristic pink color occurs at doses above 2.5 kGy, and irradiation off-odors occur above 1.5 kGy. These changes can be minimized by irradiating the shrimp when they are frozen. No significant change in protein, fat, carbohydrate, and ash content occurs as a result of low-dose irradiation. Low-dose irradiation does not produce any detectable change in the levels of volatile carbonyl compounds; irradiation at 8 kGy results in a transient increase in the total volatile compounds during subsequent storage in ice, but the increase is higher in the unirradiated shrimp. Radiation-induced changes in the fatty acid composition of shrimp are small. Some minor changes in the amino acid composition occur in irradiated shrimp; similar changes occur due to other processes such as canning and drying in hot air. Some vitamins in shrimp such as thiamine are affected by irradiation. But the loss is less extensive than in thermally processed shrimp. The protein efficiency ratio is not affected by irradiation of shrimp, and no adverse effects attributed to irradiation were found in animal feeding studies
@@ In the Chinese lunar calendar,every year corresponds with an animal.Twelve animals-rat,ox,tiger,rabbit,dragon,snake,horse,sheep,monkey,rooster,dog and pig-make up the zodiac and symbolize different years.The year 2010 is the Year of Hu,or the Year of the Tiger.
Chanchani, Pranav; Noon, Barry R; Bailey, Larissa L; Warrier, Rekha A
Tiger (Panthera tigris) conservation efforts in Asia are focused on protected areas embedded in human-dominated landscapes. A system of protected areas is an effective conservation strategy for many endangered species if the network is large enough to support stable metapopulations. The long-term conservation of tigers requires that the species be able to meet some of its life-history needs beyond the boundaries of small protected areas and within the working landscape, including multiple-use forests with logging and high human use. However, understanding of factors that promote or limit the occurrence of tigers in working landscapes is incomplete. We assessed the relative influence of protection status, prey occurrence, extent of grasslands, intensity of human use, and patch connectivity on tiger occurrence in the 5400 km(2) Central Terai Landscape of India, adjacent to Nepal. Two observer teams independently surveyed 1009 km of forest trails and water courses distributed across 60 166-km(2) cells. In each cell, the teams recorded detection of tiger signs along evenly spaced trail segments. We used occupancy models that permitted multiscale analysis of spatially correlated data to estimate cell-scale occupancy and segment-scale habitat use by tigers as a function of management and environmental covariates. Prey availability and habitat quality, rather than protected-area designation, influenced tiger occupancy. Tiger occupancy was low in some protected areas in India that were connected to extensive areas of tiger habitat in Nepal, which brings into question the efficacy of current protection and management strategies in both India and Nepal. At a finer spatial scale, tiger habitat use was high in trail segments associated with abundant prey and large grasslands, but it declined as human and livestock use increased. We speculate that riparian grasslands may provide tigers with critical refugia from human activity in the daytime and thereby promote tiger occurrence
Describes a project on culturing shrimp. Presents observational labs and the experimentation procedure. Provides general information about shrimp, their life span, optimum temperatures, and other important information. (YDS)
In 2010 a book by Amy Chua: Battle Hymn of the Tiger Mother was published and it sparked a broad discussion among pedagogues and the open society about the factors determining educational success. Chua forms a simple and provocative thesis – the Chinese mothers are the best in the world because they don’t spoil their children, quickly introduce them into the adult culture, have high expectations of them, they are brusque and cold but they teach their children how to survive and be competitive...
Soltanizadeh, Nafiseh; Mousavinejad, Mohsen S
Green tiger shrimp (Penaeus semisulcatus) is an important aquaculture species worldwide. Its perishable nature, however, needs preservation methods to ensure its quality and shelf life. In this study, the effects of Aloe vera coating on the quality and shelf life of shrimps during cold storage were investigated. Shrimp samples were dipped in aqueous solutions containing 25%, 50%, 75%, and 100% Aloe vera gel before storage at 4 °C for 7 days. Drip loss, pH, TBA, TVB-N, and texture of both the control and treated shrimp samples were analyzed periodically. There were significant differences between coated shrimps and the control group in all parameters evaluated. Aloe vera at 75% and 100% concentrations was able to prevent lipid oxidation and drip loss properly; however, coatings containing 25% Aloe vera did not have the desired effects on these characteristics. Shrimps coated with higher concentrations of Aloe vera had better textural properties during cold storage. Results also indicated the positive effects of Aloe vera coating on the sensory quality of shrimp. PMID:26396412
Kansas Data Access and Support Center — The 2012 Census TIGER/Line data originally made available by the US Census Bureau. The spatial data was then joined to the 2010 Census Summary File 1 via the common...
Earth Data Analysis Center, University of New Mexico — This data set is a vector polygon digital data structure taken from the Census Bureau's TIGER/Line Files, 1994, for New Mexico. The source software was ARC/INFO...
Tiger nut (Cyperus esculentus L.) has been recognised as one of the best nutritional crops that can be used to augment the Ghanaian diet. The application of gamma irradiation as means of preserving tiger nut could modify the characteristics of resultant flour. The purpose of this study was to determine the physicochemical, functional and pasting characteristics of flour from gamma irradiated tiger nut. The yellow and black types of tiger nut were sorted, washed and dried in an air-oven at 60 oC for 24 h. The dried tiger nut samples were irradiated at 0.0, 2.5, 5.0 and 10.0 kGy and then flours produced from them. Moisture, ash, pH, titratable acidity, water and oil absorption capacities, swelling power, solubility, bulk density and pasting properties of the flours were determined using appropriate analytical methods. Results showed that irradiation did not significantly (P>0.05) affect the moisture and ash contents of the resultant flours. Gamma irradiation significantly (P≤0.05) increased titratable acidity with concomitant decrease in pH of the flours. No significant differences were observed for water and oil absorption capacities, swelling power as well as bulk density. Solubility significantly (P≤0.05) increased generally with irradiation dose. Peak viscosity, viscosities at 92 °C and 55 °C, breakdown and setback viscosities decreased significantly with irradiation dose. Flour produced from irradiated tiger nut has a potential in complementary food formulations due to its low viscosity and increased solubility values. - Highlights: • Physicochemical, functional and pasting characteristics of flour from gamma irradiated tiger nut were studied. • Irradiation did not affect the moisture and ash contents of the resultant flours. • Titratable acidity increased with decrease in pH of the flours from the irradiated tiger nut. • Solubility increased whereas peak viscosity decreased with irradiation dose. • Flour produced from irradiated tiger nut has a
They say you never miss the water until you see the well run dry.It’s certainly true in the case of the world’s endangered species.And especially in the instance of the South China tiger.By the time the conservation authorities hit the panic button it was already too late and the big beautiful cats had disappeared from the wilds of China,culled by hunters and poachers,and squeezed out of their habitat by the march of civilization and its voracious appetite for land. Now bred in captivity and rewilded,the tigers’numbers inch upwards.Growing public awareness helps. But replacing nature’s work artificially brings its own challenges.
National Oceanic and Atmospheric Administration, Department of Commerce — The SEFSC, in cooperation with the South Atlantic states, collects South Atlantic shrimp data from dealers and fishermen. These data are collected to provide catch,...
National Oceanic and Atmospheric Administration, Department of Commerce — Gulf of Mexico Shrimp Landings - This data set contains catch (landed catch) and effort for fishing trips made by the larger vessels that fish near and offshore for...
National Oceanic and Atmospheric Administration, Department of Commerce — Brown shrimp, Farfantepenaeus aztecus, and white shrimp, Litopenaeus setiferus, were held were held under natural light conditions before experiments. Experiments...
Earth Data Analysis Center, University of New Mexico — The TIGER/Line Shapefiles are an extract of selected geographic and cartographic information from the Census MAF/TIGER database. The Census MAF/TIGER database...
Full Text Available In 2010 a book by Amy Chua: Battle Hymn of the Tiger Mother was published and it sparked a broad discussion among pedagogues and the open society about the factors determining educational success. Chua forms a simple and provocative thesis – the Chinese mothers are the best in the world because they don’t spoil their children, quickly introduce them into the adult culture, have high expectations of them, they are brusque and cold but they teach their children how to survive and be competitive. Chua shows this educational model as a contrast to the Western model, where the children have their own, naive and sentimental culture, their own shops and catwalks in shopping malls. The results of systematic research on education seem to prove something quite different. The educational success has to be measured using different scales, because it depends on different factors. The data published by OECD show that the level of education depends on the educational tradition of the society, level of GDP, intergenerational contacts, level of education of teachers and their social status. A strong determiner is the family, but not necessarily the mother. Even more, there is a strong correlation between the results in learning and a supporting stance of the parents, but also with their habit of spending free time with their children. The parents who take their children to the cinema, an a trip, gossip with them or take them to McDonalds, can be sure that their children will have statistically higher than the average grades. Detailed results from other sources show that success correlates the most with grades from previous class, parents’ habit of talking about things at school, higher education of the parents, being a child of a single mother, signing the child up for extracurricular classes from music and the mother working part-time. Failure correlates with being an Afro-American or Latino, checking homework by parents, free time after school without
Duran, Lena Ballone
Presents a brine shrimp activity designed for students in grades 5-12 to foster authentic scientific inquiry in addition to providing an engaging and exciting avenue for student exploration. Emphasizes that inquiry should be a critical component in the science classroom. (KHR)
Press the spring onion and ginger, add 25 grams water, soak for ten minutes, filter and remove the residue.Mince the shrimp meat and add pork fat. Add Shaoxing wine, egg white, spring onion and ginger juice, salt, MSG, and cornstarch, and blend. Slice the ham, and clean the bean sprouts. Put clear stock in a wok and heat.
This two-page fact sheet describes Clean Cities' technical assistance (Tiger Teams) capabilities and projects, both completed and ongoing. Tiger Teams are a critical element of the Clean Cities program, providing on-the-ground consultation to help inform program strategies. The knowledge Tiger Team experts gain from these experiences often helps inform other alternative fuels activities, such as needed research, codes and standards revisions, and new training resources.
The need for geographic information in digital form is becoming apparent in many areas, including Emergency Response Planning and Management. The Bureau of Census recognized this need and developed an automated geographic data base, known as Topologically Integrated Geographic Encoding and Referencing (TIGER) System, to produce the geographic products for the 1990 census. The Bureau makes the information available to the public on CD-ROM disks. As distributed, the geographic information is not directly visible, and a program is needed to display the information in a graphic form. MapInfo is a commercially available program that has the capability to display maps and allows the user to perform certain geographic analyses. MapInfo runs on a variety of low-cost platforms that may be found in an average office environment, as well as on high-performance work stations. The TIGER5 program developed by the Emergency Management Information Systems (EMIS) project at Oak Ridge National Laboratory reads files directly from the Census Bureau TIGER/Line CD-ROM and creates MapInfo Exchange Format Files that can be directly imported into MapInfo. The standard default values for scaling parameters are used, and the resulting map is in the same coordinate system as the world and state maps distributed with MapInfo.
Chow, Hwee Kwan
This paper considers the choices facing the Asian tiger economies regarding growth strategies that foster trans-Pacific rebalancing. A review of historical data spanning 2000 to 2008 reveals only a slight widening of the overall current account surplus but that there is considerable variation across the countries, with Hong Kong, China exhibiting the biggest increase in the saving and investment (S-I) balance. Meanwhile, cross-correlation coefficient estimates tentatively suggest that changes...
Full Text Available Recent genetic analysis has shown that the extinct Caspian Tiger (P. t. virgata and the living Amur Tigers (P. t. altaica of the Russian Far East are actually taxonomically synonymous and that Caspian and Amur groups historically formed a single population, only becoming separated within the last 200 years by human agency. A major conservation implication of this finding is that tigers of Amur stock might be reintroduced, not only back into the Koreas and China as is now proposed, but also through vast areas of Central Asia where the Caspian tiger once lived. However, under the current tiger conservation framework the 12 “Caspian Tiger States” are not fully involved in conservation planning. Equal recognition as “Tiger Range States” should be given to the countries where the Caspian tiger once lived and their involvement in tiger conservation planning encouraged. Today, preliminary ecological surveys show that some sparsely populated areas of Central Asia preserve natural habitat suitable for tigers. In depth assessments should be completed in these and other areas of the Caspian range to evaluate the possibility of tiger reintroductions. Because tigers are a charismatic umbrella species, both ecologically and politically, reintroduction to these landscapes would provide an effective conservation framework for the protection of many species in addition to tigers. And for today’s Amur Tigers this added range will provide a buffer against further loss of genetic diversity, one which will maintain that diversity in the face of selective pressures that can only be experienced in the wild.
An account of the factors that led to the collapse of the 'Celtic Tiger' economy in 2008 and an explanation of the political effects and implications for Irish identity.......An account of the factors that led to the collapse of the 'Celtic Tiger' economy in 2008 and an explanation of the political effects and implications for Irish identity....
Data available in the literature on the nutritional aspects of irradiated shrimp are reviewed and the indication is that irradiation of shrimp at doses up to about 3.2 kGy does not significantly affect the levels of its protein, fat, carbohydrate and ash. There are no reports on the effect of irradiation of shrimp above 3.2 kGy on these components. Limited information available indicates that there are some minor changes in the fatty acid composition of shrimp as a result of irradiation. Irradiation also causes some changes in the amino acid composition of shrimp; similar changes occur due to canning and hot-air drying. Some of the vitamins in shrimp, such as thiamine, are lost as a result of irradiation but the loss is less extensive than in thermally processed shrimp. Protein quality of shrimp, based on the growth of rats as well as that of Tetrahymena pyriformis, is not affected by irradiation. No adverse effects attributed to irradiation were found either in short-term or long-term animal feeding tests
Kirkpatrick, R Craig; Emerton, Lucy
The lucrative, illegal trade in tigers (Panthera tigris) remains a major conservation problem. Tiger farming has been proposed as a potential solution, with farmed tigers substituting for wild tigers. At first glance, this argument's logic seems simple: farming will increase the supply of tigers, prices will fall, and poaching will no longer be profitable. We contend, however, that this supply-side argument relies on mistaken assumptions. First, tiger markets are imperfect, meaning they are dominated by a few producers who control price. Second, consumers prefer wild tigers to farmed tigers and therefore the two are not pure substitutes. In economic terms, products from wild tigers are luxury goods, commanding a price premium. Third, there is no evidence that farmed tigers can be produced or sold more cheaply than wild tigers. In sum, it is unlikely that farming will drive down the price of wild-caught tigers or decrease profitability for tiger poachers. Rather, tiger farming is more likely to increase aggregate demand for tiger products and stimulate higher levels of poaching. PMID:20337676
Ward, G C
Wildlife protection activities in Ranthambhore in Rajasthan state in 1993, and conditions of overpopulation and expanded grazing were described. Project Tiger is an ambitious program begun in the 1970s to protect the habitat of the tiger in Ranthambhore National Park. Forest park protection is limited by corrupt, poorly trained staff, who are unmotivated to protect the established sanctuaries. Tiger counts declined from 45 in the 1980s to 25 in 1993. Between 1988 and 1993, the Ranthambhore Foundation has worked to change village attitudes and practices that are based on forest exploitation. The aim was to show how the park and livelihoods can coexist without destruction of the park through excess grazing, and to initiate the planting of trees in a largely arid, treeless region. Demonstrating new ways of living had to be achieved first by building trust. Thapar, an author and environmentalist, has established his own land as an example of how irrigation and fallow land can return the land to productive potential. A mix of Jersey and Holstein cattle are bred to show how nonrange-fed cattle can be produced and deliver higher milk yields than the straggly range-fed ones. A dairy cooperative has been organized in the district, and the milk productivity has increased 1000%. Several bio-gas cookers are used to demonstrate the effectiveness of dung for fuel, instead of wood from the forest. Women become aware of the advantages of shorter walks and the end of potential arrests from taking forest protected fuelwood. There is still work to be done in learning how to work with local people. One foundation program works with instilling ideas about conservation among 3500 village children. Forest Protection Societies have been organized to grow and defend their own trees. Village men are organized to help police unauthorized grazing in protected areas. Not all programs have been successful, and lax administration has been a problem. Tourism has become a problem of too many
Driscoll, C.A.; I. Chestin; H. Jungius; O. Pereladova; Y. Darman; E. Dinerstein; J. Seidensticker; SANDERSON, J; Christie, S.; S.J. Luo
Recent genetic analysis has shown that the extinct Caspian Tiger (P. t. virgata) and the living Amur Tigers (P. t. altaica) of the Russian Far East are actually taxonomically synonymous and that Caspian and Amur groups historically formed a single population, only becoming separated within the last 200 years by human agency. A major conservation implication of this finding is that tigers of Amur stock might be reintroduced, not only back into the Koreas and China as is now proposed, but also ...
Kerley, Linda L
This paper is a review of the history, development and efficacy of using dogs in wildlife studies and considers the use of dogs in the research and conservation of wild tigers (Panthera tigris Linnaeus, 1758). Using scat detection dogs, scent-matching dogs, law enforcement detection dogs and protection dogs are proven methods that can be effectively used on tigers. These methods all take advantage of the dog's extremely evolved sense of smell that allows them to detect animals or animal byproducts (often the focus of tiger studies). Dogs can be trained to communicate this information to their handlers. PMID:21392356
Kohler, Maxie; Aldridge, Jerry; Christensen, Lois M.; Kilgo, Jennifer
A "tiger mom" is a term popularized with the publication of Amy Chua's "Battle Hymn of the Tiger Mother" (2011). Chua points to the tiger as a "living symbol of strength and power, [which] generally inspires fear and respect" (n.p.); the term "tiger mother" or "tiger mom" quickly became part of the popular lexicon. Chua perceives the tiger mom's…
Tantius, Britta; Wittschieber, Daniel; Schmidt, Sven; Rothschild, Markus A; Banaschak, Sibylle
Two captive tiger attacks are presented that took place in Cologne and Münster zoos. Both attacks occurred when the handlers, intent on cleaning the enclosures, entered whilst the tigers accidently retained access to the location, and thus defended their territory against the perceived intruders. Both victims suffered fatal neck injuries from the bites. At Münster, colleagues managed to lure the tiger away from its victim to enable treatment, whilst the Cologne zoo tiger had to be shot in order to allow access to be gained. Whilst it was judged that human error led to the deaths of the experienced zookeepers, the investigation in Münster was closed as no third party was found to be at fault, whereas the Cologne zoo director was initially charged with being negligent. These charges were subsequently dismissed as safety regulations were found to be up to date. PMID:26115644
Kim, Su Yeong
“Tiger” parenting, as described by Amy Chua , has instigated scholarly discourse on this phenomenon and its possible effects on families. Our eight-year longitudinal study, published in the Asian American Journal of Psychology [Kim, Wang, Orozco-Lapray, Shen, & Murtuza, 2013b], demonstrates that tiger parenting is not a common parenting profile in a sample of 444 Chinese American families. Tiger parenting also does not relate to superior academic performance in children. In fact, the be...
Full Text Available Many scientists and conservation workers agree that global tiger numbers in key areas could double by 2022 if efforts are taken immediately. Countries facing declining tiger numbers and having the ability to meet this goal have produced national tiger recovery plans which outline necessary program activities that need to be taken and their associated costs. To assess the financial feasibility of tiger conservation program in Nepal and to recommend viable alternatives to secure long-term tiger conservation costs, we conducted financial analyses of tiger conservation programs in Nepal. Our results show that the present funding level fails to cover the costs of taking the recommended steps for tiger conservation in the long run. Such a finding points to the need to find and secure alternative funding sources to supply approximately a 100% increase in revenues currently generated from tiger-bearing protected areas assuming a continuance of the current level of funding by the government. This finding is troublesome given the magnitude of the financial burden associated with necessary steps to increase tiger population, plus the fact that no policy instrument exists that can target the revenue generated by the protected areas specifically for tiger conservation. To achieve financial sustainability of the tiger conservation program, alternative financial mechanisms warrants serious consideration. An alternative institutional mechanism could be a tiger conservation trust fund that would be entrusted to secure a wide range of financing from domestic and international sources to ensure financial sustainability of the Nepal’s tiger conservation program.
Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...
Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...
Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...
Myers, D L; Zurbriggen, A.; Lutz, H.; Pospischil, A.
In light of recent canine distemper virus (CDV) epidemics, we set out to determine the historical significance of CDV infection in captive lions and tigers in Switzerland. The retrospective case material consisted of 42 lion and tiger necropsy cases from 1972 to 1992. Necropsy reports for all lions and tigers were reviewed. All existing paraffin tissues were immunohistochemically examined with a polyclonal antibody raised against CDV. The results for 19 of the 42 lions and tigers were classif...
Gour, Digpal Singh; Bhagavatula, Jyotsna; Bhavanishankar, Maradani; Reddy, Patlolla Anuradha; Gupta, Jaya A.; Sarkar, Mriganka Shekhar; Hussain, Shaik Mohammed; Harika, Segu; Gulia, Ravinder; Shivaji, Sisinthy
Background Tiger populations are dwindling rapidly making it increasingly difficult to study their dispersal and mating behaviour in the wild, more so tiger being a secretive and solitary carnivore. Methods We used non-invasively obtained genetic data to establish the presence of 28 tigers, 22 females and 6 males, within the core area of Pench tiger reserve, Madhya Pradesh. This data was evaluated along with spatial autocorrelation and relatedness analyses to understand patterns of dispersal ...
Zareena Begum I; Amanat K. Gill
Despite substantial conservation investments by governments and international agencies, the existence of tigers in the wild is still threatened. The main threats to the survival of wild tigers are poaching, prey depletion, and habitat degradation and fragmentation. All international trade in tiger parts has been prohibited since 1975, with China introducing a domestic ban in 1993. The domestic trade ban in China was followed by the establishment of captive tiger breeding farms in East Asia. C...
Sun, Yujiao; Lu, Taofeng; Sun, Zhaohui; Guan, Weijun; Liu, Zhensheng; Teng, Liwei; Wang, Shuo; Ma, Yuehui
In this study, the complete mitochondrial genome of Siberian tiger (Panthera tigris altaica) was sequenced, using muscle tissue obtained from a male wild tiger. The total length of the mitochondrial genome is 16,996 bp. The genome structure of this tiger is in accordance with other Siberian tigers and it contains 12S rRNA gene, 16S rRNA gene, 22 tRNA genes, 13 protein-coding genes, and 1 control region. PMID:24660907
In August 2002, we performed MRI scans on a female juvenile Bengal tiger. We present the clinical course, imaging and autopsy findings, and some comparative anatomy of the tiger brain and skull. Magnetic resonance images of a tiger have not previously been published Copyright (2004) Blackwell Publishing Asia Pty Ltd
Mazák, Ji H.; Christiansen, Per; Kitchener, Andrew C.
The tiger is one of the most iconic extant animals, and its origin and evolution have been intensely debated. Fossils attributable to extant pantherine species-lineages are less than 2 MYA and the earliest tiger fossils are from the Calabrian, Lower Pleistocene. Molecular studies predict a much younger age for the divergence of modern tiger subspecies at
Wilting, Andreas; Courtiol, Alexandre; Christiansen, Per; Niedballa, Jürgen; Scharf, Anne K; Orlando, Ludovic; Balkenhol, Niko; Hofer, Heribert; Kramer-Schadt, Stephanie; Fickel, Jörns; Kitchener, Andrew C
Although significantly more money is spent on the conservation of tigers than on any other threatened species, today only 3200 to 3600 tigers roam the forests of Asia, occupying only 7% of their historical range. Despite the global significance of and interest in tiger conservation, global approaches to plan tiger recovery are partly impeded by the lack of a consensus on the number of tiger subspecies or management units, because a comprehensive analysis of tiger variation is lacking. We analyzed variation among all nine putative tiger subspecies, using extensive data sets of several traits [morphological (craniodental and pelage), ecological, molecular]. Our analyses revealed little variation and large overlaps in each trait among putative subspecies, and molecular data showed extremely low diversity because of a severe Late Pleistocene population decline. Our results support recognition of only two subspecies: the Sunda tiger, Panthera tigris sondaica, and the continental tiger, Panthera tigris tigris, which consists of two (northern and southern) management units. Conservation management programs, such as captive breeding, reintroduction initiatives, or trans-boundary projects, rely on a durable, consistent characterization of subspecies as taxonomic units, defined by robust multiple lines of scientific evidence rather than single traits or ad hoc descriptions of one or few specimens. Our multiple-trait data set supports a fundamental rethinking of the conventional tiger taxonomy paradigm, which will have profound implications for the management of in situ and ex situ tiger populations and boost conservation efforts by facilitating a pragmatic approach to tiger conservation management worldwide. PMID:26601191
... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission ETC Tiger Pipeline, LLC; Notice of Application June 25, 2010. Take notice that on June 15, 2010, ETC Tiger Pipeline, LLC (ETC Tiger), 711 Louisiana Street, Suite 900,...
Bhattacharjee, Subhadeep; Kumar, Vinod; Chandrasekhar, Mithileshwari; Malviya, Manjari; Ganswindt, Andre; Ramesh, Krishnamurthy; Sankar, Kalyanasundaram; Umapathy, Govindhaswamy
Tiger (Panthera tigris), an endangered species, is under severe threat from poaching, habitat loss, prey depletion and habitat disturbance. Such factors have been reported causing local extermination of tiger populations including in one of the most important reserves in India, namely Sariska Tiger Reserve (STR) in northwestern India. Consequently, tigers were reintroduced in STR between 2008 and 2010, but inadequate breeding success was observed over the years, thus invoking an investigation...
Chowdhury, Arabinda N.; Brahma, Arabinda; Mondal, Ranajit; Biswas, Mrinal K.
Aims: Human-tiger conflict (HTC) is a serious public health issue in Sundarban Reserve Forest, India. HTC is a continued concern for significant mortality and morbidity of both human and tiger population. This study examined 49 widows, whose husbands were killed by tigers, in order to explore the cultural stigma related with tiger-killing and consequent discrimination and social rejection. Different psychosocial aspects of community stigma associated with tiger-killings is discussed in the context of local culture. Methods: A mix of both quantitative and qualitative methods was used in this ethnographic study in two mouzas of Sundarban adjacent to Reserve Forest, involving (1) Village Survey for Tiger-widows, (2) In-depth interview of the widows, (3) Focus Group discussions, (4) Participatory mapping and (5) Stigma assessment by using a 28 item stigma scale especially devised for this research. For comparison of stigma-burden snake-bite widows and normal widows were taken from the same community. Results: Tiger-widows showed significantly higher stigma scores on all the clusters (fear, negative feelings, disclosure, discrimination, community attitudes, and spiritual dimension) than from both normal and snake-bite widows. They also showed higher total stigma score (65.9 ± 9.8) than normal widows (35.8 ± 8.0) and snake-bite widows (40.1 ± 7.1) and this difference was highly significant (P < 0.001). IDIs and FGDs helped to unfold the cultural construct of stigma related to tiger-killing. This can be seen in how the tiger-widows’ quality of life has been negatively impacted with a multitude of post-trauma psychological scars, deprivation, abuse and exploitation. Conclusions: The study proposes that administrative strategy for sustainable alternative income generation and conservation policy with integrated participatory forest management may save both human and tiger. A community ecocultural mental health programme addressing to eradicate the cultural stigma
Kim, Su Yeong
“Tiger” parenting, as described by Amy Chua , has instigated scholarly discourse on this phenomenon and its possible effects on families. Our eight-year longitudinal study, published in the Asian American Journal of Psychology [Kim, Wang, Orozco-Lapray, Shen, & Murtuza, 2013b], demonstrates that tiger parenting is not a common parenting profile in a sample of 444 Chinese American families. Tiger parenting also does not relate to superior academic performance in children. In fact, the best developmental outcomes were found among children of supportive parents. We examine the complexities around defining tiger parenting by reviewing classical literature on parenting styles and scholarship on Asian American parenting, along with Amy Chua’s own description of her parenting method, to develop, define, and categorize variability in parenting in a sample of Chinese American families. We also provide evidence that supportive parenting is important for the optimal development of Chinese American adolescents.
This Document contains findings identified during the Tiger Team Compliance Assessment of the Department of Energy's (DOE's) Pinellas Plant, Pinellas County, Florida. The assessment wa directed by the Department's Office of Environment, Safety, and Health (ES H) from January 15 to February 2, 1990. The Pinellas Tiger Team Compliance Assessment is comprehensive in scope. It covers the Environment Safety and Health, and Management areas and determines the plant's compliance with applicable Federal (including DOE), State, and local regulations and requirements.
Empirical measurements of the use of space of an enclosure are important indicators of the enclosure’s appropriateness for the exhibited animal. By studying the animal’s use of space zoological parks are able to provide a more species-adequate environment. In this study the utilization of space by the Siberian tigers held at Kolmården Djurpark was analyzed. Data were collected using the scan sample method for a total of 120 observation hours during 15 days. The tigers showed a marked differen...
This document presents planned actions, and their associated costs, for addressing the findings in the Environmental, Safety and Health Tiger Team Assessment of the Sandia National Laboratories, Albuquerque, May 1991, hereafter called the Assessment. This Final Action Plan should be read in conjunction with the Assessment to ensure full understanding of the findings addressed herein. The Assessment presented 353 findings in four general categories: (1)Environmental (82 findings); (2) Safety and Health (243 findings); (3) Management and Organization (18 findings); and (4) Self-Assessment (10 findings). Additionally, 436 noncompliance items with Occupational Safety and Health Administration (OSHA) standards were addressed during and immediately after the Tiger Team visit
Full text: Ion microprobes are specialised mass spectrometers that allow in situ isotopic and abundance measurements of chemically complex materials with a spatial resolution of 5 to 20 micrometers. The Sensitive High mass Resolution Ion Micro-Probe (SHRIMP II), an Australian built instrument, is designed to achieve high mass resolution (>5000 MRP at the 1% level) and great sensitivity, with detection limits in the ppb range for most elements in the Periodic Table. The SHRIMP ion microprobes have revolutionised geochronology and they are primarily used for U-Th-Pb dating of zircons from geological samples. The in situ analysis capability of the SHRIMP is now recognised as the best method for obtaining highly precise ages for zircons and other accessory minerals in ancient rocks with complex geological histories. The Western Australian SHRIMP II, which is located at Curtin University, provides rapid, precise measurements of isotope ratios in minerals and glasses and has been applied to a wide variety of geological problems and to a few applied physics problems. During the last three years we have expanded the number of minerals that can be used for U-Th-Pb age dating to include titanite, rutile, apatite, badellyite, perovskite and monazite. In addition to age dating the SHRIMP can be used for isotopic tracing. SHRIMP analysis provides the spatial resolution required to study S isotopes in very complex ores from many of Australia's largest ore deposits (Mt Isa, Broken Hill, Olympic Dam). Sulphur isotope ratios can be measured with a precision of 1-2 ppm in both sulphides and sulphates. In situ S isotopic compositions of galena (PbS), sphalerite (ZnS), chalcopyrite (CuS) and pyrite (FeS) has been crucial in the development of models of ore deposition. The in situ analytical capability and the high sensitivity of the SHRIMP II has allowed measurement of, diffusion profiles of REE and other trace element in melt couples, the mineral-melt partition coefficients of these
Sujeet Kumar Singh
Full Text Available Attacks of tigers on humans are a common feature of human-wildlife conflicts in India, and the individual identification of such animals has been an issue for management purposes. We document a case study where we established the species, sex and genetic identity of a man-eater tiger reported from Corbett Tiger Reserve (CTR, India, using blood spots and other biological samples. A man-eater tiger killed 4 women within 2 months (December 2010 to January 2011 in different parts of CTR. The authorities decided to shoot the animal, and attempts were made to do so, but it escaped. After 16 days, a tiger was shot by the management, and biological samples were collected. The multi-locus genetic profile of an injured tiger based on blood from the injured tiger was compared with that from biological samples from the shot tiger. Our results indicate that the injured and shot tigers were the same individual. This study elucidates the potential of wildlife genetics in identification of tigers involved in fatal attacks and improves the wildlife management strategies employed where the greatest numbers of direct human- tiger conflicts are taking place.
Cronin, Thomas W.; Chiou, Tsyr-Huei; Caldwell, Roy L.; Roberts, Nicholas; Marshall, Justin
While color signals are well known as a form of animal communication, a number of animals communicate using signals based on patterns of polarized light reflected from specialized body parts or structures. Mantis shrimps, a group of marine crustaceans, have evolved a great diversity of such signals, several of which are based on photonic structures. These include resonant scattering devices, structures based on layered dichroic molecules, and structures that use birefringent layers to produce circular polarization. Such biological polarizers operate in different spectral regions ranging from the near-UV to medium wavelengths of visible light. In addition to the structures that are specialized for signal production, the eyes of many species of mantis shrimp are adapted to detect linearly polarized light in the ultraviolet and in the green, using specialized sets of photoreceptors with oriented, dichroic visual pigments. Finally, a few mantis shrimp species produce biophotonic retarders within their photoreceptors that permit the detection of circularly polarized light and are thus the only animals known to sense this form of polarization. Mantis shrimps use polarized light in species-specific signals related to mating and territorial defense, and their means of manipulating light's polarization can inspire designs for artificial polarizers and achromatic retarders.
Cooney, Thomas M.
Purpose: While the growth of the Irish economy ("Celtic Tiger") has been well documented, not enough attention has been given to the role of education as a cornerstone for the success. This paper seeks to review education policy in Ireland over the past 50 years and to identify the significant educational initiatives that helped shape modern…
Reaalselt arvestades on tõenäosus, et Eestit väisab golfistaar Tiger Woods, muidugi olematu. Teoreetiliselt on see siiski võimalik. Vestlusest Jõelähtme golfiväljakut opereeriv Estonian Golf Country Clubi presidendi Mait Schmidtiga
Mention "best-seller" and author Amy Chua in the same breath and, chances are, a person's thoughts turn immediately to her controversial parenting memoir, "Battle Hymn of the Tiger Mother." Indeed, national backlash has shadowed Chua for months since publication of the book, which describes her rigid parenting style in what she loosely calls "the…
Xue, Hao-Ran; Yamaguchi, Nobuyuki; Driscoll, Carlos A; Han, Yu; Bar-Gal, Gila Kahila; Zhuang, Yan; Mazak, Ji H; Macdonald, David W; O'Brien, Stephen J; Luo, Shu-Jin
The Bali (Panthera tigris balica) and Javan (P. t. sondaica) tigers are recognized as distinct tiger subspecies that went extinct in the 1940s and 1980s, respectively. Yet their genetic ancestry and taxonomic status remain controversial. Following ancient DNA procedures, we generated concatenated 1750bp mtDNA sequences from 23 museum samples including 11 voucher specimens from Java and Bali and compared these to diagnostic mtDNA sequences from 122 specimens of living tiger subspecies and the extinct Caspian tiger. The results revealed a close genetic affinity of the 3 groups from the Sunda Islands (Bali, Javan, and Sumatran tigers P. t. sumatrae). Bali and Javan mtDNA haplotypes differ from Sumatran haplotypes by 1-2 nucleotides, and the 3 island populations define a monophyletic assemblage distinctive and equidistant from other mainland subspecies. Despite this close phylogenetic relationship, no mtDNA haplotype was shared between Sumatran and Javan/Bali tigers, indicating little or no matrilineal gene flow among the islands after they were colonized. The close phylogenetic relationship among Sunda tiger subspecies suggests either recent colonization across the islands, or else a once continuous tiger population that had subsequently isolated into different island subspecies. This supports the hypothesis that the Sumatran tiger is the closest living relative to the extinct Javan and Bali tigers. PMID:25754539
Ji H Mazák
Full Text Available The tiger is one of the most iconic extant animals, and its origin and evolution have been intensely debated. Fossils attributable to extant pantherine species-lineages are less than 2 MYA and the earliest tiger fossils are from the Calabrian, Lower Pleistocene. Molecular studies predict a much younger age for the divergence of modern tiger subspecies at <100 KYA, although their cranial morphology is readily distinguishable, indicating that early Pleistocene tigers would likely have differed markedly anatomically from extant tigers. Such inferences are hampered by the fact that well-known fossil tiger material is middle to late Pleistocene in age. Here we describe a new species of pantherine cat from Longdan, Gansu Province, China, Panthera zdanskyi sp. nov. With an estimated age of 2.55-2.16 MYA it represents the oldest complete skull of a pantherine cat hitherto found. Although smaller, it appears morphologically to be surprisingly similar to modern tigers considering its age. Morphological, morphometric, and cladistic analyses are congruent in confirming its very close affinity to the tiger, and it may be regarded as the most primitive species of the tiger lineage, demonstrating the first unequivocal presence of a modern pantherine species-lineage in the basal stage of the Pleistocene (Gelasian; traditionally considered to be Late Pliocene. This find supports a north-central Chinese origin of the tiger lineage, and demonstrates that various parts of the cranium, mandible, and dentition evolved at different rates. An increase in size and a reduction in the relative size of parts of the dentition appear to have been prominent features of tiger evolution, whereas the distinctive cranial morphology of modern tigers was established very early in their evolutionary history. The evolutionary trend of increasing size in the tiger lineage is likely coupled to the evolution of its primary prey species.
Tigers are a threatened species that might soon disappear in the wild. Not only are tigers threatened by deteriorating and declining habitat, but poachers continue to kill tigers for traditional medicine, decoration pieces and so on. Although international trade in tiger products has been banned since 1987 and domestic trade within China since 1993, tigers continue to be poached and Chinese entrepreneurs have established tiger farms in anticipation of their demise. While China desires to perm...
Richard Damania; Randy Stringer; K. Ullas Karanth; Brad Stith
The tiger (Panthera tigris) is classi- fied as endangered and populations continue to decline. This paper presents a formal economic analysis of the two most imminent threats to the survival of wild tigers: poaching tigers and hunting their prey. A model is developed to examine interactions between tigers and farm households living in and around tiger habitats. The analysis extends the existing literature on tiger demography, incorporating predator-prey interactions and exploring the sensitiv...
Short vignettes are related to show the conditions for girls and women in Morocco. Descriptions are given for child labor, literacy, the government's education campaign, youth group efforts to enhance family planning (FP) knowledge, the impact of FP outreach in rural areas, and unmarried mothers. In Morocco's cities, young boys can be seen hawking cigarettes and working in market stalls; in the countryside, boys herd goats or do other farm work. In rural areas girls are hidden by having them perform work around the house or on the farm primarily indoors. Women are supervised by women. 54% work as maids and 39% are apprentices in carpet factories. Parents prefer to have their daughters working and consider it protection from mischief as well as needed income. Only 60% of girls are enrolled in primary school vs. 80% of the boys. In rural areas, only 44% of girls are enrolled, and 20% stay to complete their primary education, while 76% of boys enroll and 63% complete primary school. Literacy of women has an effect on the ability to accurately take birth control pills. All ages of women gather at schools in the evening for lessons in reading and writing in a program supported by the King. Women are pleased with their success in just learning how to write their own names. Television advertisements promote sending children to school, as another part of the Ministry of Education's campaign to increase girl's educational status. There are still not enough schools; many schools are double shift, and communities are building their own schools. Youth clubs, which refer to boys as "tiger cubs" and girls as "little flowers," try to familiarize young people with some basic information about contraception. A traditional midwife relates some problems with girl's education: costs for clothing and supplies, worry about male teachers, and poor role models. In some remote areas, farm families do not send their children to school, because of the distance to schools and the need for
... Recovery (TIGER) AGENCY: Department of Transportation, Maritime Administration. ACTION: Notice of... Generating Economic Recovery (TIGER) grant. An environmental assessment (EA) and FONSI have been...
Peng, Zhiwei; Liu, Shijie; Hou, Zhijun; Xing, Mingwei
The Siberian tiger is endangered and is listed by the International Union for the Conservation of Nature; the captive environment is utilized to maintain Siberian tiger numbers. Little information regarding the prevalence of parasites in Siberian tigers is available. A total of 277 fecal samples of Siberian tigers were analyzed in this study. The microscopic analysis indicated the presence of ascarid eggs of Toxascaris leonina and Toxocara cati. The ascarid infection rate was 67.5% in Siberian tigers. The internal transcribed spacer-1 (ITS-1) phylogenetic analysis indicated that T. leonina belonged to Toxascaris and that Toxo. cati belonged to Toxocara. The infestation rate and intensity of T. leonina were higher than those of Toxo. cati. One-way analysis of variance showed that the presence of T. leonina was significantly associated with age (Pparasitic diseases among other tigers in the zoo. PMID:27514888
Full Text Available Eight traditional subspecies of tiger (Panthera tigris, of which three recently became extinct, are commonly recognized on the basis of geographic isolation and morphological characteristics. To investigate the species' evolutionary history and to establish objective methods for subspecies recognition, voucher specimens of blood, skin, hair, and/or skin biopsies from 134 tigers with verified geographic origins or heritage across the whole distribution range were examined for three molecular markers: (1 4.0 kb of mitochondrial DNA (mtDNA sequence; (2 allele variation in the nuclear major histocompatibility complex class II DRB gene; and (3 composite nuclear microsatellite genotypes based on 30 loci. Relatively low genetic variation with mtDNA, DRB, and microsatellite loci was found, but significant population subdivision was nonetheless apparent among five living subspecies. In addition, a distinct partition of the Indochinese subspecies P. t. corbetti into northern Indochinese and Malayan Peninsula populations was discovered. Population genetic structure would suggest recognition of six taxonomic units or subspecies: (1 Amur tiger P. t. altaica; (2 northern Indochinese tiger P. t. corbetti; (3 South China tiger P. t. amoyensis; (4 Malayan tiger P. t. jacksoni, named for the tiger conservationist Peter Jackson; (5 Sumatran tiger P. t. sumatrae; and (6 Bengal tiger P. t. tigris. The proposed South China tiger lineage is tentative due to limited sampling. The age of the most recent common ancestor for tiger mtDNA was estimated to be 72,000-108,000 y, relatively younger than some other Panthera species. A combination of population expansions, reduced gene flow, and genetic drift following the last genetic diminution, and the recent anthropogenic range contraction, have led to the distinct genetic partitions. These results provide an explicit basis for subspecies recognition and will lead to the improved management and conservation of these recently
Johnson, Brennan; Garrity, Deborah; Dasi, Lakshmi Prasad
We investigate the fluid dynamics of brine shrimp larvae swimming in this gallery of fluid motion video. Time resolved particle image velocimetry was performed using nano-particles as seeding material to measure the time dependent velocity and vorticity fields. The Reynolds number of the flow was roughly 8 and the Womerseley number (ratio of periodic forcing to viscous forcing) was about 5. Vorticity dynamics reveals the formation of a vortex ring structure at the tip of each arm at the begin...
Chowdhury, Arabinda N.; Ranajit Mondal; Arabinda Brahma; Biswas, Mrinal K.
AIMS Human–tiger conflict (HTC) is a serious public health issue in Sundarban Reserve Forest, India. HTC is a continued concern for the significant mortality and morbidity of both human and tiger population. This is the first comprehensive report on Sundarban tiger–human conflicts and its impact on widows whose husbands were killed by tigers. The study attempts to explore the situation analysis of HTC and the aftermath of the incident including bereavement and coping, the cultural stigma rela...
Driscoll, Carlos A.; Yamaguchi, Nobuyuki; Bar-Gal, Gila Kahila; Roca, Alfred L.; Luo, Shujin; Macdonald, David W.; O'Brien, Stephen J.
The Caspian tiger (Panthera tigris virgata) flourished in Central Asian riverine forest systems in a range disjunct from that of other tigers, but was driven to extinction in 1970 prior to a modern molecular evaluation. For over a century naturalists puzzled over the taxonomic validity, placement, and biogeographic origin of this enigmatic animal. Using ancient-DNA (aDNA) methodology, we generated composite mtDNA haplotypes from twenty wild Caspian tigers from throughout their historic range ...
Tigers (Panthera tigris tigris) can be viewed as a proxy for intact and healthy ecosystems. Their wild populations have plummeted to fewer than 3,200 individuals in the last four decades and threats to these apex predators are mounting rather than diminishing. Global conservation bodies (Global Tiger Initiative, World Wildlife Fund, Wildlife Conservation Society, Panthera etc.) have recently called for solidarity and scaling up of conservation efforts to save tigers from extinction. In So...
If you're one of the many Unix developers drawn to Mac OS X for its Unix core, you'll find yourself in surprisingly unfamiliar territory. Unix and Mac OS X are kissing cousins, but there are enough pitfalls and minefields in going from one to another that even a Unix guru can stumble, and most guides to Mac OS X are written for Mac aficionados. For a Unix developer, approaching Tiger from the Mac side is a bit like learning Russian by reading the Russian side of a Russian-English dictionary. Fortunately, O'Reilly has been the Unix authority for over 25 years, and in Mac OS X Tiger for Unix Gee
Sujeet Kumar Singh; Vipin Sharma; Sudhanshu Mishra; Puneet Pandey; Ved Prakash Kumar; Surendra Prakash Goyal
Attacks of tigers on humans are a common feature of human-wildlife conflicts in India, and the individual identification of such animals has been an issue for management purposes. We document a case study where we established the species, sex and genetic identity of a man-eater tiger reported from Corbett Tiger Reserve (CTR), India, using blood spots and other biological samples. A man-eater tiger killed 4 women within 2 months (December 2010 to January 2011) in different parts of CTR. The au...
The fitness industry is blooming, and people are keen on healthy lifestyles. Training centers attract more and more people nowadays. Tourists travel to Phuket, Thailand for training camps lasting several weeks. A well-known training center, Tiger Muay Thai, offers a wide range of fitness classes and working out premises and in addition accommodation, recreational activities, and food services. The goals of this study are to find out the level of customer satisfaction towards the services o...
The main objective of the thesis was to assess the level of customer satisfaction of the airline company Tiger Airways, which is a low-cost airline with a considerable number of dissatisfied customers. In the study the theories of customer satisfaction were reviewed for providing solutions for the airline to reduce the number of discontented customers. To analyze the current situation of the airline company’s customer satisfaction the quantitative research method was used. The research ma...
Bhanwar Vishvendra Raj Singh
Full Text Available Accelerating deforestation, rampant poaching and mining is leading to habitat destruction of wildlife animals especially carnivore’s animals like Tiger, which is further aggravating the problem of man-animal conflict. So that’s why The tiger population in world and India has been decreasing at an alarming rate over the past 100 years, with 40,000 wild tigers roaming the landscape in the early 20th century, to only 1,700 dotting the landscape today. Meanwhile India government has made great strides recently to protect tigers and increase population numbers through tiger reserves, pu-nishing poachers with imprisonment and fines, and through effective patrolling strategies. In this paper more and more focus on what is scenario is going on regarding with tiger population. As well as the discussed issue, history, scientific characteristic, habitat and distribution, diet, threat and conservation of tiger management. In this paper major focus on spatial temporal analysis in tiger population in India.
... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission ETC Tiger Pipeline, LLC; Notice of Filing January 13, 2011. Take notice that on December 30, 2010, ETC Tiger Pipeline, LLC submitted a request for a waiver of the...
Full Text Available Although translocation has been used in mitigating human-carnivore conflict for decades, few studies have been conducted on the behavioral ecology of released animals. Such information is necessary in the context of sustainable forest management. In this study we determine the type of land cover used as main habitat and examine the activity pattern of translocated tigers. Between 2008 and 2010 we captured six conflict tigers and translocated them 74-1,350 km from their capture sites in Sumatera. All tigers were fitted with global positioning system (GPS collars. The collars were set to fix 24-48 location coordinates per day. All translocated tigers showed a preference for a certain habitat type within their new home range, and tended to select the majority of natural land cover type within the landscape as their main habitat, but the availability of natural forest habitat within the landscape remains essensial for their survival. The activity of male translocated tigers differed significantly between the six time intervals of 24 hours, and their most active periods were in the afternoon (14:00-18:00 hours and in the evening (18:00-22:00 hours. Despite being preliminary, the findings of this study-which was the first such study conducted in Sumatera-highlight the conservation value of tiger translocation and provide valuable information for improving future management of conflict tigers.Keywords: activity pattern, GPS collars, habitat selection, sumateran tiger, translocation
Enceladus, the sixth-largest moon of Saturn, is a cold, icy world but its also remarkably active. Recent studies have charted over a hundred geysers venting gas and dust into space from Enceladus south polar region. New research addresses the question of how the moons extreme surface terrain influences the locations and behavior of these geysers.Active PlumesEnceladus orbiting within Saturns E ring. Enceladus plumes probably created this ring. [NASA/JPL/Space Science Institute]A decade ago, scientists discovered that Enceladus south polar region is home to a prominent set of four fractures known as the tiger stripes. This region was found to contain roughly 100 geyser jets, which form plumes of gas and dust venting into space at a combined rate of ~200 kilograms per second! These plumes are probably the source of the material in Saturns E ring, in which Enceladus orbits.Recently, Carolyn Porco (UC Berkeley and CICLOPS Space Science Institute) led a study that analyzed 6.5 years of Cassini data, surveying the locations and orientations of 101 geysers. The outcome was peculiar: the geysers are distributed along the tiger stripes, but their directions are not all pointing vertically from the surface (see the video below!).Now, Paul Helfenstein (Cornell University) has teamed up with Porco to examine whether the surface terrain surrounding the geysers affects where the jets erupt, what direction they point, and even when theyre active.Surface InfluenceHelfenstein and Porco demonstrate that the locations and behavior of the geysers are very likely influenced by Enceladus surface features in this region. In particular, they find:The spacing of the geyser jets on Enceladus is not random.The jets are roughly uniformly distributed along the three most active tiger stripes, spaced about 5 kilometers apart. This fixed spacing might be due to shear fractures produced by fault motion along the tiger stripes cutting across the stripes at regular intervals and providing
Abbott, B.; Kooten, van G.C.
Tigers are a threatened species that might soon disappear in the wild. Not only are tigers threatened by deteriorating and declining habitat, but poachers continue to kill tigers for traditional medicine, decoration pieces and so on. Although international trade in tiger products has been banned sin
deNolfo, Georgia; Binns, W. R.; Israel, M. H.; Christian, E. R.; Mitchell, J. W.; Hams, T.; Link, J. T.; Sasaki, M.; Labrador, A. W.; Mewaldt, R. A.; Stone, E. C.; Waddington, C. J.; Wiedenbeck, M. E.
Super-TIGER (Super Trans-Iron Galactic Element Recorder) is a new long-duration balloon-borne instrument designed to test and clarify an emerging model of cosmic-ray origins and models for atomic processes by which nuclei are selected for acceleration. A sensitive test of the origin of cosmic rays is the measurement of ultra heavy elemental abundances (Z > or equal 30). Super-TIGER is a large-area (5 sq m) instrument designed to measure the elements in the interval 30 TIGER builds on the heritage of the smaller TIGER, which produced the first well-resolved measurements of elemental abundances of the elements Ga-31, Ge-32, and Se-34. We present the Super-TIGER design, schedule, and progress to date, and discuss the relevance of UH measurements to cosmic-ray origins.
Hebda, Toni; Calderone, Terri L
The Technology Informatics Guiding Education Reform (TIGER) Initiative is designed to address a set of skills that is needed by all nurses who will practice in the profession in the 21st century. The skill set includes informatics competencies that range from basic computer skills to advanced-level information technology and literacy competencies and expertise. Despite the significance of the TIGER Initiative, few nurse educators have operationalized TIGER or adopted its plan to transform nursing practice and education to better prepare nurses to practice in a technology rich healthcare environment. TIGER is currently in phase III: implementation. The authors outline the TIGER Initiative as well as actions that nurse educators can take to develop and integrate informatics competencies into the curriculum to prepare nurses for the high-touch, high-technology patient-centered care of the 21st century. PMID:20173588
Damania, R.; Stringer, R.; Karanth, K.U.; Stith, B.
The tiger (Panthera tigris) is classified as endangered and populations continue to decline. This paper presents a formal economic analysis of the two most imminent threats to the survival of wild tigers: poaching tigers and hunting their prey. A model is developed to examine interactions between tigers and farm households living in and around tiger habitats. The analysis extends the existing literature on tiger demography, incorporating predator-prey interactions and exploring the sensitivity of tiger populations to key economic parameters. The analysis aims to contribute to policy debates on how best to protect one of the world's most endangered wild cats.
Weijun Mai; Chaoqun Hu
The gene coding for lysozyme in blue shrimp (Litopenaeus stylirostris) was cloned, sequenced and expressed in pET-32a vector. The deduced amino acid sequence of F. Merguiensi lysozyme showed 37-93% similarity with the mouse, human, chicken, and tiger prawn counterparts. The lysozyme was purified to homogeneity and observed as a band of approximately 15 kDa in 15% SDS-PAGE. Semi-quantitative RT-PCR analysis demonstrated that mRNA transcripts of lysozyme could be mainly detected in the tissues of haemocytes, gill, gonad and the lymphoid organ of unchallenged shrimps, whereas the expression of lysozyme transcripts was increased in all the tested tissues after the heat-killed Vibrio alginolyticus challenge. The temporal expression of lysozyme mRNA in haemolymph challenged by Micrococcus luteus and V. Alginolyticus was both up-regulated and reached the maximum level at 8 and 16 h post-stimulation, respec-tively, and then dropped back to the original level. Bacteriolytic activity of the lysozyme against different bacterial cultures was deter-mined by the solid phase and turbidimetric assays. The results demonstrated that the lysozyme we obtained was not only against Gram-positive and Gram-negative bacteria but also against shrimp pathogens V. Alginolyticus and V. Parahemolyticus. In addition, the study of the inhibition mechanism revealed that the antibacterial activity of the lysozyme was a result of the bactericidal effect.
Kannapiran, E.; Ravindran, J.; Chandrasekar, R.; Kalaiarasi, A.
and V. harveyi density were low at that time. Vaseeharan and Ramasamy (2003) also reported that the growth of pathogenic V. harveyi isolated from Penaeus monodon with black gill disease was controlled by non-pathogenic B. subtilis BT23 as probiotic...: Vibrios associated with Penaeus chinensis (Crustacea: Decapoda) larvae and post-larvae in Chinese shrimp hatcheries. Aquaculture 169, 121-132 (1998). Vaseeharan, B. and P. Ramasamy: Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible...
ERDEM, Mehmet Emin
This paper presents data on the mineral and heavy metal contents of warty crab and brown shrimp caught along Sinop coast of the Middle Black Sea. Mineral and heavy metal analysis of the samples were done by TUBITAK Marmara Scientific Research Center (Gebze-TURKEY). Minerals were determined according to AOAC (1998); heavy metals were determined according to AOAC (1999); arsenic (Ar) was determined according to EPA (1992) and mercury (Hg) quantities were determined according to EPA (1994) metho...
Bhattacharjee, Subhadeep; Kumar, Vinod; Chandrasekhar, Mithileshwari; Malviya, Manjari; Ganswindt, Andre; Ramesh, Krishnamurthy; Sankar, Kalyanasundaram; Umapathy, Govindhaswamy
Tiger (Panthera tigris), an endangered species, is under severe threat from poaching, habitat loss, prey depletion and habitat disturbance. Such factors have been reported causing local extermination of tiger populations including in one of the most important reserves in India, namely Sariska Tiger Reserve (STR) in northwestern India. Consequently, tigers were reintroduced in STR between 2008 and 2010, but inadequate breeding success was observed over the years, thus invoking an investigation to ascertain physiological correlates. In the present study, we report glucocorticoid stress responses of the reintroduced tigers in relation to anthropogenic disturbance in the STR from 2011 to 2013. We found anthropogenic disturbance such as encounter rates of livestock and humans, distance to roads and efforts to kill domestic livestock associated with an elevation in fecal glucocorticoid metabolite (fGCM) concentrations in the monitored tigers. In this regard, female tigers seem more sensitive to such disturbance than males. It was possible to discern that tiger's fGCM levels were significantly positively related to the time spent in disturbed areas. Resulting management recommendations include relocation of villages from core areas and restriction of all anthropogenic activities in the entire STR. PMID:26061171
MacMillan, Douglas C.
This paper explores village-based tiger killing (TK) among communities bordering the Sundarbans mangrove forest, Bangladesh. We find that TKs are not purely retaliatory in nature (i.e. a desire for retribution following livestock depredation or attacks on humans by tigers) and that previous negative experience of tigers is not the sole determinant of villagers’ acceptance of TK behaviour. Inter-related social-psychological factors (risk perceptions, beliefs about tigers, tolerance for tigers)...
Kapfer, Paul M.; Streby, Henry M.; Gurung, B.; Simcharoen, A.; McDougal, C.C.; Smith, J.L.D.
Attempts to conserve declining tiger Panthera tigris populations and distributions have experienced limited success. The poaching of tiger prey is a key threat to tiger persistence; a clear understanding of tiger diet is a prerequisite to conserve dwindling populations. We used unpublished data on tiger diet in combination with two previously published studies to examine fine-scale spatio-temporal changes in tiger diet relative to prey abundance in Chitwan National Park, Nepal, and aggregated data from the three studies to examine the effect that study duration and the size of the study area have on estimates of tiger diet. Our results correspond with those of previous studies: in all three studies, tiger diet was dominated by members of Cervidae; small to medium-sized prey was important in one study. Tiger diet was unrelated to prey abundance, and the aggregation of studies indicates that increasing study duration and study area size both result in increased dietary diversity in terms of prey categories consumed, and increasing study duration changed which prey species contributed most to tiger diet. Based on our results, we suggest that managers focus their efforts on minimizing the poaching of all tiger prey, and that future studies of tiger diet be of long duration and large spatial extent to improve our understanding of spatio-temporal variation in estimates of tiger diet. ?? 2011 Wildlife Biology, NKV.
Berthelote, A. R.
American Indian tribes and tribal confederations exert sovereignty over about 20% of all the freshwater resources in the United States. Yet only about 30 Native American (NA) students receive bachelor's degrees in the geosciences each year, and few of those degrees are in the field of hydrology. To help increase the ranks of NA geoscientists,TIGER builds upon the momentum of Salish Kootenai College's newly accredited Hydrology Degree Program. It allows for the development and implementation of the first Bachelor's degree in geosciences (hydrology) at a Tribal College and University (TCU). TIGER integrates a solid educational research-based framework for retention and educational preparation of underrepresented minorities with culturally relevant curriculum and socio-cultural supports, offering a new model for STEM education of NA students. Innovative hydrology curriculum is both academically rigorous and culturally relevant with concurrent theoretical, conceptual, and applied coursework in chemical, biological, physical and managerial aspects of water resources. Educational outcomes for the program include a unique combination of competencies based on industry recognized standards (e.g., National Institute of Hydrologists), input from an experienced External Advisory Board (EAB), and competencies required for geoscientists working in critical NA watersheds, which include unique competencies, such as American Indian Water Law and sovereignty issues. TIGER represents a unique opportunity to capitalize on the investments the geoscience community has already made into broadening the participation of underrepresented minorities and developing a diverse workforce, by allowing SKC to develop a sustainable and exportable program capable of significantly increasing (by 25 to 75%) the National rate of Native American geoscience graduates.
Menz, William J.; Penna, Matthew J.; Biggs, Mark J.
Replica-exchange molecular dynamics (REMD) must be used to enhance sampling when there are significant (relative to kT) barriers between different parts of the phase space. TIGER2 is a next-generation REMD method that offers more efficient sampling compared to the original REMD method by reducing the number of replicas required to span a given temperature range. In this paper, we present an implementation of the TIGER2 algorithm in the NAMD software package. This implementation exploits the capacity of NAMD to interpret Tcl scripts. The Tcl script implementing TIGER2 is provided and explained in detail, and demonstrated for alanine dipeptide in water. Program summaryProgram title: TNAMD Catalogue identifier: AEHH_v1_0 Program summary URL:http://cpc.cs.qub.ac.uk/summaries/AEHH_v1_0.html Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: Standard CPC licence, http://cpc.cs.qub.ac.uk/licence/licence.html No. of lines in distributed program, including test data, etc.: 24 837 No. of bytes in distributed program, including test data, etc.: 610 107 Distribution format: tar.gz Programming language: Tcl 8.5 Computer: Clusters, workstations. Tested on Intel Clovertown, Atom. Operating system: Linux, Windows (with Linux shell) Has the code been vectorised or parallelised?: Yes. Parallelised with MPI Classification: 3 External routines: NAMD 2.6 ( http://www.ks.uiuc.edu/Research/namd/) Nature of problem: Replica-exchange molecular dynamics. Solution method: Replicas are assigned to unique processes, and pass through a series of cycles of heating, equilibration, quenching and sampling; after which temperatures are swapped between replicas. Running time: The test run can take up to twenty minutes.
NSGIC GIS Inventory (aka Ramona) — ROADS_TIGER05_IN is a line shapefile of Indiana roads. Original data are from the U.S. Department of Commerce, U.S. Census Bureau, Census 2005 (First Edition) Tiger...
Full Text Available In Asia, there is, in general, a great reverence held for the tiger. The tiger has been imitated and reigns supreme as king of all the beasts throughout Asia. The relationship between man and tiger holds a strange duality in that as much as the tiger is feared for its fierce savagery and destructive power, it is also revered for these very same qualities and for its majestic nature. Therefore, the very symbolic essence of the tiger has permeated all levels of the Asian community and culture; art, mythology, religion, astrology, herbology, and military fighting strategies. The purpose of this article is to show the many rich aspects that the tiger exhibits, and its influence and impact on Asian culture and Chinese martial arts in particular. Martial arts such as Cantonese Hung Gar (Hong Family and Hasayfu Hung Gar (Hong Family Four Lower Tigers dedicate a portion of their systems to achieving awesome strength and speed, and to imitating the tiger’s physical prowess. By doing so, they may achieve higher levels of effectiveness within the martial arts.
The survival rates of shrimp larvae in different stage are higher than those of control groups when probiotic bacteria strains X4B-1 and X1B-1 which are isolated from gut of adult shrimp are added into the little volume rearing sea water of shrimp larvae. The effects of probiotic bacteria are evaluated by challenge test (pathogenic bacteria Z3G2 isolated from disease shrimp larvae in the hatchery of Jimo town) and low salinity stress resistance tests on shrimp larvae, the survival rate and lengths of the shrimp larvae in the experiment are determined. Results indicate that 1. The survival rate, ability of resistant to low salinity, lengths of the delivered shrimp larvae are improved after the strains of probiotic bacteria, X4B-1 or X1B-1, are added into the rearing sea water of hatchery. 2. The addition of the probiotic bacteria could not influence the change of the bacteria number, NH3-N and COD value in the rearing sea water. 3. The probiotic bacteria used in the experiment have many enzymes such as Lipase, Amylase, Gelatinase and Lecithinase. These enzymes may help the probiotic bacteria to digest the food components fed to shrimp larvae and increase the digestive efficiency of post larvae. This may be one of the reasons why these probiotic bacteria are beneficial to the shrimp larvae.
Full Text Available Even with global support for tiger (Panthera tigris conservation their survival is threatened by poaching, habitat loss and isolation. Currently about 3,000 wild tigers persist in small fragmented populations within seven percent of their historic range. Identifying and securing habitat linkages that connect source populations for maintaining landscape-level gene flow is an important long-term conservation strategy for endangered carnivores. However, habitat corridors that link regional tiger populations are often lost to development projects due to lack of objective evidence on their importance. Here, we use individual based genetic analysis in combination with landscape permeability models to identify and prioritize movement corridors across seven tiger populations within the Central Indian Landscape. By using a panel of 11 microsatellites we identified 169 individual tigers from 587 scat and 17 tissue samples. We detected four genetic clusters within Central India with limited gene flow among three of them. Bayesian and likelihood analyses identified 17 tigers as having recent immigrant ancestry. Spatially explicit tiger occupancy obtained from extensive landscape-scale surveys across 76,913 km(2 of forest habitat was found to be only 21,290 km(2. After accounting for detection bias, the covariates that best explained tiger occupancy were large, remote, dense forest patches; large ungulate abundance, and low human footprint. We used tiger occupancy probability to parameterize habitat permeability for modeling habitat linkages using least-cost and circuit theory pathway analyses. Pairwise genetic differences (FST between populations were better explained by modeled linkage costs (r>0.5, p<0.05 compared to Euclidean distances, which was in consonance with observed habitat fragmentation. The results of our study highlight that many corridors may still be functional as there is evidence of contemporary migration. Conservation efforts should
Bhanwar Vishvendra Raj Singh; Ashok Kumar
Accelerating deforestation, rampant poaching and mining is leading to habitat destruction of wildlife animals especially carnivore’s animals like Tiger, which is further aggravating the problem of man-animal conflict. So that’s why The tiger population in world and India has been decreasing at an alarming rate over the past 100 years, with 40,000 wild tigers roaming the landscape in the early 20th century, to only 1,700 dotting the landscape today. Meanwhile India government h...
Wen, Rong; Li, Fuhua; Li, Shihao; Xiang, Jianhai
JAK/STAT signaling pathway plays key roles in the antiviral immunity of mammals, fish and insect. However, limited knowledge is known about the function of JAK/STAT signaling pathway in the antiviral immunity of shrimp although virus disease has caused severe mortality in shrimp aquaculture. In order to understand the function of JAK/STAT signaling pathway in the antiviral immunity of shrimp, dsRNA interfering technique was used to silence the expression of STAT gene in Litopenaeus vannamei, and the mortality of shrimp was detected after WSSV infection. Furthermore, the expressions of some potential target genes regulated by STAT or genes related to RNA interfering pathway were detected in STAT silenced shrimp during WSSV infection. The WSSV copy number in STAT silenced shrimp was 10(2)-10(3) copies/ng DNA which was much lower than that in the control. The mortality in STAT silenced shrimp caused by WSSV infection decreased very significantly compared to their controls. The function of STAT was verified in vitro cultured cells of hematopoietic tissue of crayfish Cherax quadricarinatus by adding specific inhibitor of STAT3(S3I-201), and the cultured cells treated with S3I-201 showed much less WSSV copy number than their controls, which further suggested that STAT might be helpful for the replication of WSSV. Expression analysis on the potential STAT target genes and genes in RNA interfering pathway provide important information for understanding the functional mechanism of STAT in antiviral immunity of shrimp. PMID:24727196
Chapuis L.; Bshary R.
Signals increase the fitness of a sender by altering the behaviour of receivers. For cooperative interactions biological market theory proposes that signalling strength may be linked to supply and demand. In this context, a recent laboratory experiment demonstrated that cleaner shrimps may advertise their service to client reef fish and that the advertisement is linked to hunger levels. We investigated signalling by the cleaner shrimp Periclimenes longicarpus in the field to test more detaile...
Johnson, Brennan; Dasi, Lakshmi Prasad
We investigate the fluid dynamics of brine shrimp larvae swimming in this gallery of fluid motion video. Time resolved particle image velocimetry was performed using nano-particles as seeding material to measure the time dependent velocity and vorticity fields. The Reynolds number of the flow was roughly 8 and the Womerseley number (ratio of periodic forcing to viscous forcing) was about 5. Vorticity dynamics reveals the formation of a vortex ring structure at the tip of each arm at the beginning of the power stroke. This two vortex system evolves dramatically with time as the stroke progresses. The outer circulation is noted to weaken while the inner circulation strengthens over the power stroke. The gaining strength of the inner vortex correlates with the acceleration and forward movement of the larvae.
Inês Maria Barbosa Nunes Queiroga
Full Text Available The objective of this research was to evaluate the sensory quality of the marine shrimp Litopenaeus vannamei grown and stored in a freezer. A sensory analysis consisted of the Quality Index Method (MIQ to review the raw shrimp and Descriptive Analysis (AD in cooked shrimp in samples stored for a period of 90 days, using eight previously trained panelists. Accommodation comprising shrimp filet (100-120 pieces / kg samples were subjected to freezing in liquid nitrogen (- 86 °C, Freezing Tunnel (- 35 °C and Domestic Freezer (- 18 °C. At 0, 30, 60 and 90 days of storage in these freezing systems, determination of pH, water holding capacity (WHC, weight loss during cooking (WLC, shearing force, color, total volatile bases (TVB and thiobarbituric acid reactive substances (TBARS were performed. The attributes manual firmness and softness showed better results in rapid freezing. The color parameters (a *, b * and L *, WHC and WCL were higher at 90 days of storage, no significant losses were observed. Highlighted the strength of greater shear in slowly frozen samples at 90 days, confirming the results reported by the sensory panel. At 90 days of storage, the frozen shrimp showed good sensory quality and physical and chemistry characteristics. The shelf life of this shrimp could be set at about 90 days.
Pitsko, Leigh Elizabeth
Humans maintain wild animals in zoological parks for the purposes of education,conservation, research, and recreation. However, abnormal behaviors may develop in animals housed in human-made environments, if those environments do not allow them to carry out their natural behaviors (such as swimming, climbing, stalking, and predation). Captive environments in zoological parks often do not provide for natural behaviors due to spatial constraints and negative public reaction. Tigers (Panthera ...
NSGIC GIS Inventory (aka Ramona) — The TIGER/Line Files are shapefiles and related database files (.dbf) that are an extract of selected geographic and cartographic information from the U.S. Census...
NSGIC GIS Inventory (aka Ramona) — The TIGER/Line Files are shapefiles and related database files (.dbf) that are an extract of selected geographic and cartographic information from the U.S. Census...
Ishac, Joseph A.
This technical report intends to highlight the key findings and recommendations of the SpaceWire Tiger Team for the CoNNeCT project. It covers findings which are technical in nature, covering design concepts and approaches.
November 6, 2010: Top Chinese luxury brand NE.TIGER launched a grand opening ceremony for its image store in Shanghai. The new store is located at Libao Square on Middle Huaihai Road, which has a commer-
U.S. Geological Survey, Department of the Interior — City locations for all places in the TIGER files; this file was extracted from dbf files posted on the internet by the Bureau of the Census. This is basically a...
NSGIC GIS Inventory (aka Ramona) — The TIGER/Line Files are shapefiles and related database files (.dbf) that are an extract of selected geographic and cartographic information from the U.S. Census...
"New Tigers" (including city commercial banks) outperform state-owned commercial banks burdened with non-performing loans from unprofitable state-owned enterprises. We study whether this is due solely to superior corporate governance (multiple shareholders versus total government ownership) or also to the favorable environment (the New Tigers target affluent China, while state-owned commercial banks operate nationwide). Using a field survey on 20 city commercial banks from three provinces at ...
Dolly Priatna; Yanto Santosa; Lilik Budi Prasetyo; Agus Priyono Kartono
Although translocation has been used in mitigating human-carnivore conflict for decades, few studies have been conducted on the behavioral ecology of released animals. Such information is necessary in the context of sustainable forest management. In this study we determine the type of land cover used as main habitat and examine the activity pattern of translocated tigers. Between 2008 and 2010 we captured six conflict tigers and translocated them 74-1,350 km from their capture sites in Sumate...
GONO SEMIADI; R. TAUFIQ PURNA NUGRAHA
The Sumatran tiger (Panthera tigris sumatrae) is one of several endemic Indonesian wild cat groups which population is critically endangered. A program to increase the population size had been conducted in captivity, especially in the zoo. In order to monitor the captive population and for the means of management in captivity, a logbook data recording system had been developed for individual animals. A compilation data from the Tiger International Stud Book from 1942 to 2000 was analyzed. The...
Schmidtke, Gerhard; Avakyan, S.V.; Berdermann, J.; Bothmer, V.; Cessateur, G.; Ciraolo, L.; Didkovsky, L.; Dudok de Wit, Thierry; Eparvier, F. G.; Gottwald, A.; Haberreiter, M.; Hammer, R.; Jacobi, Ch.; N. Jakowski; Kretzschmar, M.
At the 10th Thermospheric Ionospheric GEospheric Research (TIGER/COSPAR) symposium held in Moscow in 2014 the achievements from the start of TIGER in 1998 were summarized. During that period, great progress was made in measuring, understanding, and modeling the highly variable UV-Soft X-ray (XUV) solar spectral irradiance (SSI), and its effects on the upper atmosphere. However, after more than 50 years of work the radiometric accuracy of SSI observation is still an issue and requires further ...
The tropomyosin fraction of shrimp proteins is potentially responsible for allergic reaction in individuals with genetic predisposition to allergy. However, there are no efficient and safe methods to reduce its allergenicity. High intensity ultrasound is known to change the structure of proteins. This study is aimed at assessing high intensity ultrasound's effect on the allergenicity of shrimp allergen. Shrimp and purified shrimp allergen were treated with high intensity ultrasound for 30～180 min. Extracts of treated samples were analyzed by enzyme-linked immunosorbent assay (ELISA) with pool serum of shrimp allergy patients and polyclonal anti-allergen antibodies and by immunoblotting after polyacrylamide gel electrophoresis. Shrimp treated with high intensity ultrasound showed a decrease in allergenicity measured with ELISA. A linear relationship between the immune response induced by treated shrimp allergen and the applied treatment time was observed. The decrease in allergenicity was confirmed by immunoblot assays with shrimp allergic patients serum. Allergenicity of shrimp allergen extracted from treated shrimp was higher than that of purified shrimp allergen with the same treatment time. Gel-filtration HPLC was applied for analysis of shrimp allergen after treatment with high intensity ultrasound. Some fractions were appeared with increasing treatment time. The results suggested that high intensity ultrasound could be used to reduce the allergenicity of shrimp.
Full Text Available We evaluated the potential of adult fairy shrimp, Streptocephalus sirindhornae, as live food for growth and enhancing the carotenoid contents in the giant freshwater prawn Macrobrachium rosenbergii. Completely randomized cultures of 6-months-old prawns were treated individually with 5 different food ratios (adult fairy shrimp to dry shrimp diet; 0: 100, 25: 75, 50: 50, 75: 25 and 100: 0 for 60 days in circular black plastic containers containing 20 L of water. The diet was fed at a rate of 3% of the prawn body weight per day. Each food concentration was experimented with 12 replicates and the whole experiment (5 treatments using a total of 60 individuals. The results showed that the initial lengths (11.9-13.0 cm and weights (23.20-31.65 g of the experimental prawns with the 5 food ratios were not significantly different. However, length (0.87 cm and weight (15.14 g gains of the prawns fed pure fairy shrimp treatment (100: 0 were significantly different from those of the other dietary treatments (p-1 of fresh weight with large amounts of astaxanthin and -carotene, which was 2.8 times of those fed with pure dry diet treatment (p<0.05. Present results indicate improved growth performances and elevated carotenoid contents of prawns suggesting fairy shrimp as promising feeds in freshwater aquaculture.
Joshi, Anup R; Dinerstein, Eric; Wikramanayake, Eric; Anderson, Michael L; Olson, David; Jones, Benjamin S; Seidensticker, John; Lumpkin, Susan; Hansen, Matthew C; Sizer, Nigel C; Davis, Crystal L; Palminteri, Suzanne; Hahn, Nathan R
The global population of wild tigers remains dangerously low at fewer than 3500 individuals. Habitat loss, along with poaching, can undermine the international target recovery of doubling the number of wild tigers by 2022. Using a new satellite-based monitoring system, we analyzed 14 years of forest loss data within the 76 landscapes (ranging from 278 to 269,983 km(2)) that have been prioritized for conservation of wild tigers. Our analysis provides an update of the status of tiger habitat and describes new applications of technology to detect precisely where forest loss is occurring in order to curb future habitat loss. Across the 76 landscapes, forest loss was far less than anticipated (79,597 ± 22,629 km(2), 7.7% of remaining habitat) over the 14-year study period (2001-2014). Habitat loss was unevenly distributed within a subset of 29 landscapes deemed most critical for doubling wild tiger populations: 19 showed little change (1.5%), whereas 10 accounted for more than 98% (57,392 ± 16,316 km(2)) of habitat loss. Habitat loss in source population sites within 76 landscapes ranged from no loss to 435 ± 124 km(2) ([Formula: see text], SD = 89, total = 1676 ± 476 km(2)). Doubling the tiger population by 2022 requires moving beyond tracking annual changes in habitat. We highlight near-real-time forest monitoring technologies that provide alerts of forest loss at relevant spatial and temporal scales to prevent further erosion. PMID:27051881
Louisiana Geographic Information Center — This is a line data set for 'Railroads' of Louisiana extracted from 1997 TIGER/Line source data. Railroad Mainlines, Spurs, Yards, and specialized rail lines have...
Full Text Available Today, most wild tigers live in small, isolated Protected Areas within human dominated landscapes in the Indian subcontinent. Future survival of tigers depends on increasing local population size, as well as maintaining connectivity between populations. While significant conservation effort has been invested in increasing tiger population size, few initiatives have focused on landscape-level connectivity and on understanding the effect different landscape elements have on maintaining connectivity. We combined individual-based genetic and landscape ecology approaches to address this issue in six protected areas with varying tiger densities and separation in the Central Indian tiger landscape. We non-invasively sampled 55 tigers from different protected areas within this landscape. Maximum-likelihood and Bayesian genetic assignment tests indicate long-range tiger dispersal (on the order of 650 km between protected areas. Further geo-spatial analyses revealed that tiger connectivity was affected by landscape elements such as human settlements, road density and host-population tiger density, but not by distance between populations. Our results elucidate the importance of landscape and habitat viability outside and between protected areas and provide a quantitative approach to test functionality of tiger corridors. We suggest future management strategies aim to minimize urban expansion between protected areas to maximize tiger connectivity. Achieving this goal in the context of ongoing urbanization and need to sustain current economic growth exerts enormous pressure on the remaining tiger habitats and emerges as a big challenge to conserve wild tigers in the Indian subcontinent.
Joshi, Aditya; Vaidyanathan, Srinivas; Mondol, Samrat; Edgaonkar, Advait; Ramakrishnan, Uma
Today, most wild tigers live in small, isolated Protected Areas within human dominated landscapes in the Indian subcontinent. Future survival of tigers depends on increasing local population size, as well as maintaining connectivity between populations. While significant conservation effort has been invested in increasing tiger population size, few initiatives have focused on landscape-level connectivity and on understanding the effect different landscape elements have on maintaining connectivity. We combined individual-based genetic and landscape ecology approaches to address this issue in six protected areas with varying tiger densities and separation in the Central Indian tiger landscape. We non-invasively sampled 55 tigers from different protected areas within this landscape. Maximum-likelihood and Bayesian genetic assignment tests indicate long-range tiger dispersal (on the order of 650 km) between protected areas. Further geo-spatial analyses revealed that tiger connectivity was affected by landscape elements such as human settlements, road density and host-population tiger density, but not by distance between populations. Our results elucidate the importance of landscape and habitat viability outside and between protected areas and provide a quantitative approach to test functionality of tiger corridors. We suggest future management strategies aim to minimize urban expansion between protected areas to maximize tiger connectivity. Achieving this goal in the context of ongoing urbanization and need to sustain current economic growth exerts enormous pressure on the remaining tiger habitats and emerges as a big challenge to conserve wild tigers in the Indian subcontinent. PMID:24223132
Gilbert, Martin; Soutyrina, Svetlana V; Seryodkin, Ivan V; Sulikhan, Nadezhda; Uphyrkina, Olga V; Goncharuk, Mikhail; Matthews, Louise; Cleaveland, Sarah; Miquelle, Dale G
Canine distemper virus (CDV) has recently been identified in populations of wild tigers in Russia and India. Tiger populations are generally too small to maintain CDV for long periods, but are at risk of infections arising from more abundant susceptible hosts that constitute a reservoir of infection. Because CDV is an additive mortality factor, it could represent a significant threat to small, isolated tiger populations. In Russia, CDV was associated with the deaths of tigers in 2004 and 2010, and was coincident with a localized decline of tigers in Sikhote-Alin Biosphere Zapovednik (from 25 tigers in 2008 to 9 in 2012). Habitat continuity with surrounding areas likely played an important role in promoting an ongoing recovery. We recommend steps be taken to assess the presence and the impact of CDV in all tiger range states, but should not detract focus away from the primary threats to tigers, which include habitat loss and fragmentation, poaching and retaliatory killing. Research priorities include: (i) recognition and diagnosis of clinical cases of CDV in tigers when they occur; and (ii) collection of baseline data on the health of wild tigers. CDV infection of individual tigers need not imply a conservation threat, and modeling should complement disease surveillance and targeted research to assess the potential impact to tiger populations across the range of ecosystems, population densities and climate extremes occupied by tigers. Describing the role of domestic and wild carnivores as contributors to a local CDV reservoir is an important precursor to considering control measures. PMID:25939829
This report documents the Tiger Assessment of the Ames Laboratory (Ames), located in Ames, Iowa. Ames is operated for the US Department of Energy (DOE) by Iowa State University. The assessment was conducted from February 10 to March 5, 1992, under the auspices of the Office of Special Projects, Office of the Assistant Secretary of Environment, Safety and Health, Headquarters, DOE. The assessment was comprehensive, encompassing Environment, Safety, and Health (ES ampersand H) disciplines; management practices; and contractor and DOE self-assessments. Compliance with applicable Federal, State of Iowa, and local regulations; applicable DOE Orders; best management practices; and internal requirements at Ames Laboratory were assessed. In addition, an evaluation of the adequacy and effectiveness of DOE and the site contractor's management of ES ampersand H/quality assurance program was conducted
This report documents the Tiger Assessment of the Ames Laboratory (Ames), located in Ames, Iowa. Ames is operated for the US Department of Energy (DOE) by Iowa State University. The assessment was conducted from February 10 to March 5, 1992, under the auspices of the Office of Special Projects, Office of the Assistant Secretary of Environment, Safety and Health, Headquarters, DOE. The assessment was comprehensive, encompassing Environment, Safety, and Health (ES H) disciplines; management practices; and contractor and DOE self-assessments. Compliance with applicable Federal, State of Iowa, and local regulations; applicable DOE Orders; best management practices; and internal requirements at Ames Laboratory were assessed. In addition, an evaluation of the adequacy and effectiveness of DOE and the site contractor's management of ES H/quality assurance program was conducted.
Rizzardi, M.; Mohr, M.S.; Merrill, D.W.; Selvin, S. (Lawrence Berkeley Lab., CA (United States) California Univ., Berkeley, CA (United States). School of Public Health)
In 1990, the United States Bureau of the Census released detailed geographic base files known as TIGER/Line (Topologically Integrated Geographic Encoding and Referencing) which contain detail on the physical features and census tract boundaries of every county in the United States. The TIGER database is attractive for two reasons. First, it is publicly available through the Bureau of the Census on tape or cd-rom for a minimal fee. Second, it contains 24 billion characters of data which describe geographic features of interest to the Census Bureau such as coastlines, hydrography, transportation networks, political boundaries, etc. Unfortunately, the large TIGER database only provides raw alphanumeric data; no utility software, graphical or otherwise, is included. On the other hand New S, a popular statistical software package by AT T, has easily operated functions that permit advanced graphics in conjunction with data analysis. New S has the ability to plot contours, lines, segments, and points. However, of special interest is the New S function map and its options. Using the map function, which requires polygons as input, census tracts can be quickly selected, plotted, shaded, etc. New S graphics combined with the TIGER database has obvious potential. This paper reports on our efforts to use the TIGER map files with New S, especially to construct census tract maps of counties. While census tract boundaries are inherently polygonal, they are not organized as such in the TIGER database. This conversion of the TIGER line'' format into New S polygon/polyline'' format is one facet of the work reported here. Also we discuss the selection and extraction of auxiliary geographic information from TIGER files for graphical display using New S.
Foodborne illness outbreaks occasionally occur as a result of microbiologically contaminated crustaceans, including shrimp. Foodborne pathogens occasionally found on shrimp include Listeria monocytogenes, Salmonella spp., Staphylococcus aureus, and Vibrios. In this study the microbiological qualit...
National Oceanic and Atmospheric Administration, Department of Commerce — This mail survey collected economic data on inshore commercial shrimp fishermen who held licenses to commercially harvest shrimp in state waters of the U.S. Gulf of...
刘海玲; 杨春莉; 杨春瑜
With the rapid development of the world’s fisheries, shrimp consumption and trade are occupying an increasingly important position in the international aquatic products market. China is the world’s largest producer of shrimp and is also the main exporter of shrimp, however, compared with developed countries, technology level of shrimp production and processing in our country is still low at present, especially in aspect of shrimp preservation and storage techniques. So it is necessary to study various technologies and applications of sterilization and conclude the effective methods of shrimp preservation and storage. Traditional storage, chemical and biological technology, low temperature preservation, heat treatment, gas preservation and physical sterilization technology are reviewed in this paper based on shrimp preservation aiming to provide certain theoretical reference and practical basis for shelf life extension of shrimp.
For decades, astronauts on space missions have documented land use changes around the world. In this pair of images, astronauts track the development of shrimp farming along the Honduran coastline of the Gulf of Fonseca between 1989 and 2001. Mariculture, primarily shrimp farming, has become a leading agricultural effort in Honduras. The regional transformation of large tracts of coastal swamps into shrimp farms blossomed throughout the 1990s. The top image was taken with color infrared film in 1989. Dense vegetation, like the coastal mangrove swamps and the forested slopes of Volcan Cosiguina show up as dark red. The bottom image, taken with color visible film by the crew of the most recent Space Shuttle mission in December 2001 shows that hundreds of square kilometers of coastal swamp, primarily in Honduras, have been converted to shrimp ponds. These appear as the light-colored, rectilinear land use pattern. The Honduras shrimp farms were hit hard by flooding after Hurricane Mitch in 1998, and a devastating virus in 1999-2000. It is not known how many of the ponds in this view are still functional. A vigorous debate continues about the sustainability of the shrimp farms and the impacts to the environment and coastal ecosystem due to mangrove clearing and mariculture waste production. Apart from the shrimp farms, the other prominent feature on these images is the impressive volcano Cosiguina, which erupted explosively in 1859 (the largest recorded eruption in the Western Hemisphere). Photograph STS-108-717-85 was taken in the December 2001 by the crew of Space Shuttle mission 108 using a Hasselblad camera with 250-mm lens. Photograph STS030-93-15 was taken in May 1989 using a Hasselblad camera and color infrared film. Both images are provided by the Earth Sciences and Image Analysis Laboratory at Johnson Space Center. Additional images taken by astronauts and cosmonauts can be viewed at the NASA-JSC Gateway to Astronaut Photography of Earth.
Javid Ali; Bashir Ahmad
Objective: To evaluate brine shrimp lethality assay of solvent extracts (aqueous, methanol, ethanol, acetone, ethyl acetate, chloroform and n-hexane) of Hippophae rhamnoides (H. rhamnoides) leaves. Methods: Brine shrimp cytotoxicity assay was used to assess the cytotoxic potential of H. rhamnoides leaves extracts. Three vials for concentration of each extract were made and 10 shrimps per vial (30 shrimps per dilution) were transferred to specific concentration of each extrac...
Food irradiation technology was conducted to reduce shrimp allergy. The experiment was designated in 3 portions as follows; A, the irradiation of raw shrimp; B the irradiation of shrimp and then cooking; and C, cooking the shrimp and then irradiation. Gamma irradiation was done with doses of 1, 3, 5, 7, 10 kGy. A shrimp sarcoplasmic protein solution (SSPS) and a myofibrillar protein solution (SMPS) were prepared from A portion. Cooked shrimp protein solutions were also prepared from B and C portions. The binding abilities of the shrimp allergic patients' IgE and mouse monoclonal Ab 4.9.5 (mAb 4.9.5), produced to the shrimp heat-stable protein, to each sample solution were determined by ELISA. Binding abilities of patients' IgE and mAb 4.9.5 to irradiated shrimp fractions were dose-dependently reduced. The cooking treatment after irradiation was more effective than the irradiation treatment after cooking in the reduction of the binding abilities of IgE and IgG. SDS-PAGE was performed to compare irradiated shrimp proteins with non-irradiated shrimp proteins. SDS-PAGE showed that no bands were changed by gamma irradiation. The results indicated that food irradiation with an adequate dose can be reduce allergenicity of shrimp
Two lots of shrimp paste from commercial source in Samutsakhon were irradiated at a recommended minimum dose of 6 kGy using a J S 8900 cobalt-60 carrier gamma irradiator of Thai Irradiation Center in Patum Thani. Red Perspex dosimeter were used to measure the absorbed dose throughout the product with emphasis on the region of minimum and maximum absorbed dose. This way, it was aimed to compare the dose effects of gamma irradiation on the microbiological, chemical and sensory quality of shrimp paste. The results indicated that the shrimp paste received minimum and maximum absorbed dose of 6.85 and 12.83 kGy with dose uniformity ratio of 1.87 . Throughput rate is 468 kilogram per hour. The microbiological load of shrimp paste was rather high resulting in not compliance with Thai industrial standard 1080-2535. Irradiation at 6.8 kGy reduced total viable bacterial count by one log cycle. Although the irradiated product was organoleptic ally acceptable and could be kept for 16 months at room temperature, mold and Clostridium perfringens were still present in some samples after irradiation and during prolonged storage in amount that exceeds the limitation of Thai industrial standard. Chemical properties such as p H, moisture and sodium chloride content of irradiated shrimp paste were not significantly changed after irradiation
Over the last few decades, there has been an increase in consumer demand for shrimp, which has resulted in its worldwide aquaculture production. In the United States, the stringent enforcement of environmental regulations encourages shrimp farmers to develop new technologies, such as recirculating raceway systems. This is a zero-water exchange system capable of producing high-density shrimp yields. The system also produces wastewater characterized by high levels of ammonia, nitrate, nitrite, and organic carbon, which make waste management costs prohibitive. Shrimp farmers have a great need for a waste management method that is effective and economical. One such method is the sequencing batch reactor (SBR). A SBR is a variation of the activated sludge biological treatment process. This process uses multiple steps in the same reactor to take the place of multiple reactors in a conventional treatment system. The SBR accomplishes equalization, aeration, and clarification in a timed sequence in a single reactor system. This is achieved through reactor operation in sequences, which includes fill, react, settle, decant, and idle. A laboratory scale SBR was successfully operated using shrimp aquaculture wastewater. The wastewater contained high concentrations of carbon and nitrogen. By operating the reactors sequentially, namely, aerobic and anoxic modes, nitrification and denitrification were achieved as well as removal of carbon. Ammonia in the waste was nitrified within 4 days. The denitrification of nitrate was achieved by the anoxic process, and 100% removal of nitrate was observed within 15 days of reactor operation. PMID:19396482
Tigers (Panthera tigris Linnaeus, 1758) are in danger of extinction. Their populations have declined from ~100,000 to only ~3,000 individuals in a century and their habitat has shrunk to less than 7% of the historic range. Of the five extant tiger subspecies, the Sumatran tiger (Panthera tigris sumatrae Pocock, 1929) is the most seriously threatened. Currently determined as Critically Endangered under IUCN criteria, the Sumatran tiger is likely to become extinct unless effective conservation ...
Dolly Priatna; Yanto Santosa; Lilik B. Prasetyo; Kartono, Agus P.
The ranging behaviour of translocated problem tigers is poorly understood. The demand for releasing problem tigers back to the wild increases following the increasing the number of problem tigers that needs to be rescued in Sumatra in the last decade. In this study we estimate the home range size and obtain information on daily range of four translocated problem tigers, as well as discussing some potential factors determining the size of home range and their movement. We translocated four ad...
Dasgupta, Susmita; Hammer, Dan; Kraft, Robin; Wheeler, David
As the wild tiger population in tropical Asia dropped from about 100,000 to 3,500 in the last century, the need to conserve tiger habitats poses a challenge for the Global Tiger Recovery Program. This paper develops and uses a high-resolution monthly forest clearing database for 74 tiger habitat areas in ten countries to investigate habitat threats for Bengal, Indochinese, Malayan and Suma...
Seimon, Tracie A.; Miquelle, Dale G.; Chang, Tylis Y.; Newton, Alisa L.; Korotkova, Irina; Ivanchuk, Galina; Lyubchenko, Elena; Tupikov, Andre; Slabe, Evgeny; McAloose, Denise
ABSTRACT Fewer than 500 Amur tigers (Panthera tigris altaica) remain in the wild. Due to low numbers and their solitary and reclusive nature, tiger sightings across their range in the Russian Far East and China are rare; sightings of sick tigers are rarer still. Serious neurologic disease observed in several wild tigers since 2001 suggested disease emergence in this endangered species. To investigate this possibility, histology, immunohistochemistry (IHC), in situ hybridization (ISH), and rev...
Mondal, Pinki; Nagendra, Harini
Protected areas (PAs) are cornerstones of biodiversity conservation, but small parks alone cannot support wide-ranging species, such as the tiger. Hence, forest dynamics in the surrounding landscapes of PAs are also important to tiger conservation. Tiger landscapes often support considerable human population in proximity of the PA, sometimes within the core itself, and thus are subject to various land use activities (such as agricultural expansion and road development) driving habitat loss and fragmentation. We synthesize information from 27 journal articles in 24 tiger landscapes to assess forest-cover dynamics in tiger-range countries. Although 29% of the PAs considered in this study have negligible change in overall forest cover, approximately 71% are undergoing deforestation and fragmentation. Approximately 58% of the total case studies have human settlements within the core area. Most changes—including agricultural expansion, plantation, and farming (52%), fuelwood and fodder collection (43%), logging (38%), grazing (38%), and tourism and development (10%)—can be attributed to human impacts largely linked to the nature of the management regime. This study highlights the need for incorporating new perspectives, ideas, and lessons learned locally and across borders into management plans to ensure tiger conservation in landscapes dominated by human activities. Given the increasing isolation of most parks due to agricultural, infrastructural, and commercial developments at the periphery, it is imperative to conduct planning and evaluation at the landscape level, as well as incorporate multiple actors and institutions in planning, instead of focusing solely on conservation within the PAs as is currently the case in most tiger parks.
Mondal, Pinki; Nagendra, Harini
Protected areas (PAs) are cornerstones of biodiversity conservation, but small parks alone cannot support wide-ranging species, such as the tiger. Hence, forest dynamics in the surrounding landscapes of PAs are also important to tiger conservation. Tiger landscapes often support considerable human population in proximity of the PA, sometimes within the core itself, and thus are subject to various land use activities (such as agricultural expansion and road development) driving habitat loss and fragmentation. We synthesize information from 27 journal articles in 24 tiger landscapes to assess forest-cover dynamics in tiger-range countries. Although 29% of the PAs considered in this study have negligible change in overall forest cover, approximately 71% are undergoing deforestation and fragmentation. Approximately 58% of the total case studies have human settlements within the core area. Most changes-including agricultural expansion, plantation, and farming (52%), fuelwood and fodder collection (43%), logging (38%), grazing (38%), and tourism and development (10%)-can be attributed to human impacts largely linked to the nature of the management regime. This study highlights the need for incorporating new perspectives, ideas, and lessons learned locally and across borders into management plans to ensure tiger conservation in landscapes dominated by human activities. Given the increasing isolation of most parks due to agricultural, infrastructural, and commercial developments at the periphery, it is imperative to conduct planning and evaluation at the landscape level, as well as incorporate multiple actors and institutions in planning, instead of focusing solely on conservation within the PAs as is currently the case in most tiger parks. PMID:21786183
Full Text Available The tiger population that once inhabited the Korean peninsula was initially considered a unique subspecies (Panthera tigris coreensis, distinct from the Amur tiger of the Russian Far East (P. t. altaica. However, in the following decades, the population of P. t. coreensis was classified as P. t. altaica and hence forth the two populations have been considered the same subspecies. From an ecological point of view, the classification of the Korean tiger population as P. t. altaica is a plausible conclusion. Historically, there were no major dispersal barriers between the Korean peninsula and the habitat of Amur tigers in Far Eastern Russia and northeastern China that might prevent gene flow, especially for a large carnivore with long-distance dispersal abilities. However, there has yet to be a genetic study to confirm the subspecific status of the Korean tiger. Bone samples from four tigers originally caught in the Korean peninsula were collected from two museums in Japan and the United States. Eight mitochondrial gene fragments were sequenced and compared to previously published tiger subspecies’ mtDNA sequences to assess the phylogenetic relationship of the Korean tiger. Three individuals shared an identical haplotype with the Amur tigers. One specimen grouped with Malayan tigers, perhaps due to misidentification or mislabeling of the sample. Our results support the conclusion that the Korean tiger should be classified as P. t. altaica, which has important implications for the conservation and reintroduction of Korean tigers.
... Fish and Wildlife Service Proposed Low-Effect Habitat Conservation Plan for the California Tiger... listed animal, the threatened Central California Distinct Population Segment of the California tiger salamander (tiger salamander). The applicant would implement a conservation program to minimize and...
... SECURITY Coast Guard Certificate of Alternative Compliance for the Offshore Supply Vessel GULF TIGER AGENCY... Compliance was issued for the offshore supply vessel GULF TIGER as required by 33 U.S.C. 1605(c) and 33 CFR... TIGER. Full compliance with 72 COLREGS and the Inland Rules Act would hinder the vessel's ability...
Lynam, Antony J.; Khaing, Saw Tun; Zaw, Khin Maung
A century ago, tigers were considered pests in Myanmar. Hunters claimed thousands, yet populations persisted. In the past century, because of habitat loss and prey depletion, coupled with the recent demand for traditional medicines, tiger populations have been reduced to a few hundred individuals. As a first step toward long-term planning for tigers, and to guide efforts to increase protected area coverage, the Myanmar government in 1998 initiated a project to develop a revised National Tiger Action Plan. Extensive surveys confirmed tigers in only 4 of 17 survey locations. Significant populations are thought to persist in the far North and far South of the country, where large, intact forests offer the potential for tiger recovery. With partnerships and collaborations, tiger populations can be protected in the short term (enforcement staff to reduce poaching of tigers and prey, and amending existing wildlife legislation in accordance with international laws. Over the long term (5-20 years), recovery of Myanmar’s tiger populations will depend on increasing support from local people, zoning tiger areas to reduce habitat loss and disturbance, and maintaining connectivity of existing national and transboundary forests. This article reviews the development of a new National Tiger Action Plan for the Union of Myanmar and discusses a blueprint for conservation measures aimed at saving tigers from extinction.
Chowdhury, Arabinda N.; Mondal, Ranajit; Brahma, Arabinda; Biswas, Mrinal K.
AIMS Human–tiger conflict (HTC) is a serious public health issue in Sundarban Reserve Forest, India. HTC is a continued concern for the significant mortality and morbidity of both human and tiger population. This is the first comprehensive report on Sundarban tiger–human conflicts and its impact on widows whose husbands were killed by tigers. The study attempts to explore the situation analysis of HTC and the aftermath of the incident including bereavement and coping, the cultural stigma related to being killed by a tiger and the consequent discrimination, deprivation, and social rejection, and the impact on the mental health of the tiger-widows. METHODS This is a three-phase ethnographic research with a mix of quantitative and qualitative methods. In the first phase, a door-to-door village survey (3,084 households) was carried out in two villages of Sundarban, which are adjacent to the Reserve Forest, in which the incidents of human–animal conflicts and the 65 tiger-widows identified were documented. In the second phase, the 65 tiger-widows were studied to explore the ecodemography of tiger attacks and tiger-widows alongside the stigma issue by using a stigma questionnaire (n = 49). The stigma burden was compared with normal widows (n = 21) and snake-bite widows (n = 18). In the third phase, the psychosocial and cultural dimensions related to tiger attacks were studied by using in-depth interviews (IDI) of the tiger-widows, focus-group discussions (FGD), and participatory mapping in the community. Clinical examinations of the mental health of the widows were also carried out in this phase. RESULTS The mean age of the 65 widows was 43.49 ± 9.58 years. Of this, 12.3% of the widows had remarried and only 4.6% of the widows were literate. In all, 67.2% of all tiger attacks occurred as a result of illegal forest entry. The main livelihood of the former husbands of the widows were 43.8% wood cutting, 28.1% fishing, 10.9% crab catching, 9.4% tiger prawn seed
This study is part of the project on the ''Quality Assurance of Different Shrimp Species for Export''. Local shrimp samples were collected from Ministry of Livestock and Fisheries and various private enterprises. Contents of biogenic amines were determined by using benzoyl chloride derivatization method with HPLC (reverse phase high performance liquid chromatography). Based on the biogenic amines, quality index of shrimps were correlated with freshness index so that the grade of shrimp samples can be classified as excellent, good, and acceptable. All sizes of shrimps such as extra large, large, medium were found to excceptable respectively
Cheng, Winton; Liu, Chun-Hung; Tsai, Chiung-Hui; Chen, Jiann-Chu
A lipopolysaccharide- and beta-1,3-glucan binding protein (LGBP) cDNA was cloned from the haemocyte and hepatopancreas of white shrimp Litopenaeus vannamei using oligonucleotide primers and RT-PCR. Both 3'- and 5'-regions were isolated by rapid amplification of cDNA end RACE method. Analysis of nucleotide sequence revealed that the cDNA clone has an open reading frame of 1101 bp encoding a protein of 367 amino acids including a 17 amino acid signal peptide. The calculated molecular mass of the mature proteins (350 amino acids) is 39.92 kDa with an estimated pI of 4.37. Two putative integrin binding motifs (cell adhesion site), RGD (Arg-Gly-Asp) and a potential recognition motif for beta- (1-->3) linkage of polysaccharides were observed in the LGBP. Sequence comparison showed that LGBP deduced amino acid of L. vannamei has an overall similarity of 95%, 92% and 61% to that of blue shrimp Litopenaeus stylirostris LGBP, tiger shrimp Penaeus monodon BGBP and crayfish Pacifastacus leniusculus LGBP, respectively. Quantitative real-time RT-PCR analysis showed that LGBP transcript in haemocyte of L. vannamei increased in 3- and 6-h post Vibrio alginolyticus injection. PMID:15561560
The possible use of gamma irradiation at doses of 2 and 3 kGy to improve bacteriological quality of frozen shrimp was investigated. The effects of gamma irradiation on salmonella viability in frozen shrimp and on sensory quality of frozen shrimp were also evaluated. Treatment of frozen shrimp at 3 kGy reduced bacterial load by 2 log cycles and eliminated Enterobacteriaceae, E. coli, V. parahaemolyticus, and S. aureus. Coliform was still present in a 3 kGy treated samples but in a very small percentage and the count was less than 10 cell/g. Salmonella was not detected in either irradiated or non-irradiated frozen shrimp. A difference in radiation resistance was noted among five salmonella serotypes tested. S. lexington was the most resistant and S. anatum was the least resistant. Salmonella resistance was higher in frozen inoculated shrimp than in refrigerated inoculated shrimp. A dose of at least 4.2 kGy is required for a seven log cycle reduction of salmonella contamination in frozen shrimp. Off-flavors were produced in frozen shrimp irradiated at 4.2 kGy but diminished during subsequent frozen storage. However, irradiated frozen shrimp was of acceptable quality for at least four months. Dosage at 3 kGy appeared to be sufficient for improving bacteriological quality of frozen shrimp
The Office Special Projects within the Office of Environment, Safety, and Health (EH) has the responsibility to conduct Tiger Team Assessments for the Secretary of Energy. This report presents the assessment of the buildings, facilities, and activities under the DOE/Rockwell Contract No. DE-AM03-76SF00700 for the Energy Technology Engineering Center (ETEC) and of other DOE-owned buildings and facilities at the Santa Susana Field Laboratory (SSFL) site in southeastern Ventura County, California, not covered under Contract No. DE-AM03-76SF00700, but constructed over the years under various other contracts between DOE and Rockwell International. ETEC is an engineering development complex operated for DOE by the Rocketdyne Division of Rockwell International Corporation. ETEC is located within SSFL on land owned by Rockwell. The balance of the SSFL complex is owned and operated by Rocketdyne, with the exception of a 42-acre parcel owned by the National Aeronautics and Space Administration (NASA). The primary mission of ETEC is to provide engineering, testing, and development of components related to liquid metals technology and to conduct applied engineering development of emerging energy technologies.
The Office Special Projects within the Office of Environment, Safety, and Health (EH) has the responsibility to conduct Tiger Team Assessments for the Secretary of Energy. This report presents the assessment of the buildings, facilities, and activities under the DOE/Rockwell Contract No. DE-AM03-76SF00700 for the Energy Technology Engineering Center (ETEC) and of other DOE-owned buildings and facilities at the Santa Susana Field Laboratory (SSFL) site in southeastern Ventura County, California, not covered under Contract No. DE-AM03-76SF00700, but constructed over the years under various other contracts between DOE and Rockwell International. ETEC is an engineering development complex operated for DOE by the Rocketdyne Division of Rockwell International Corporation. ETEC is located within SSFL on land owned by Rockwell. The balance of the SSFL complex is owned and operated by Rocketdyne, with the exception of a 42-acre parcel owned by the National Aeronautics and Space Administration (NASA). The primary mission of ETEC is to provide engineering, testing, and development of components related to liquid metals technology and to conduct applied engineering development of emerging energy technologies
Neutrinos are present in the natural environment due to terrestrial, solar, and cosmic sources and are also produced at accelerators both incidentally and intentionally as part of physics research programs. Progress in fundamental physics research has led to the creation of beams of neutrinos of ever-increasing intensity and/or energy. The large size and cost associated with these beams attracts, and indeed requires, public interest, support, and some understanding of the 'exotic' particles produced, including the neutrinos. Furthermore, the very word neutrino ('little neutral one', as coined by Enrico Fermi) can lead to public concern due to confusion with 'neutron', a word widely associated with radiological hazards. Adding to such possible concerns is a recent assertion, widely publicized, that neutrinos from astronomical events may have led to the extinction of some biological species. Presented here are methods for conservatively estimating the dose equivalent due to neutrinos as well as an assessment of the possible role of neutrinos in biological extinction processes. It is found that neutrinos produced by the sun and modern particle accelerators produce inconsequential dose equivalent rates. Examining recent calculations concerning neutrinos incident upon the earth due to stellar collapse, it is concluded that it is highly unlikely that these neutrinos caused the mass extinctions of species found in the paleontological record. Neutrino radiation hazards are, then, truly a 'paper tiger'. 14 refs., 1 fig., 1 tab
Langkjær, Michael Alexander
Pop musicians performing in black stage costume take advantage of cultural traditions relating to matters black. Stylistically, black is a paradoxical color: although a symbol of melancholy, pessimism, and renunciation, black also expresses minimalist modernity and signifies exclusivity (as...... is hinted by Rudyard Kipling’s illustration of ‘The [Black] Cat That Walked by Himself’ in his classic children’s tale). It was well understood by uniformed Anarchists, Fascists and the SS that there is an assertive presence connected with the black-clad figure. The paradox of black’s abstract elegance......-styled references to, among other things, the culturally and ideologically effervescent interwar-period have made me curious as to what alternative possibilities – for instance ‘emancipation’ – a comparative analysis might disclose concerning the visual rhetoric of black. Thus, in conclusion, it is briefly...
Li, Q; Lu, T F; Liu, D; Hu, P F; Sun, B; Ma, J Z; Wang, W J; Wang, K F; Zhang, W X; Chen, J; Guan, W J; Ma, Y H; Zhang, M H
The Amur tiger is a unique endangered species in the world, and thus, protection of its genetic resources is extremely important. In this study, an Amur tiger placenta cDNA library was constructed using the SMART cDNA Library Construction kit. A total of 508 colonies were sequenced, in which 205 (76%) genes were annotated and mapped to 74 KEGG pathways, including 29 metabolism, 29 genetic information processing, 4 environmental information processing, 7 cell motility, and 5 organismal system pathways. Additionally, PLAC8, PEG10 and IGF-II were identified after screening genes from the expressed sequence tags, and they were associated with placental development. These findings could lay the foundation for future functional genomic studies of the Amur tiger. PMID:25299101
Aguilar, Núria; Albanell, Elena; Miñarro, Begoña; Guamis, Buenaventura; Capellas, Marta
Tiger nut is a tuber used to produce tiger nut milk that yields a high quantity of solid waste, which can be dried and used as fiber source. The objective of this paper was to evaluate the quality of gluten-free bread formulated with different tiger nut-derived products in order to substitute soya flour (which is an allergen ingredient) and, at the same time, increase the use of tiger nut-derived products. Four gluten-free formulations based on corn starch and containing tiger nut milk, tiger nut milk by-product, tiger nut flour, or soya flour (as reference formulation) were studied. Tiger nut milk increased G' of gluten-free batter and rendered breads with the softest crumb (502.46 g ± 102.05), the highest loaf-specific volume (3.35 cm(3)/g ± 0.25), and it was mostly preferred by consumers (61.02%). Breads elaborated with tiger nut flour had similar characteristics than soya flour breads (except in color and crumb structure). The addition of tiger nut milk by-product resulted in a hard (1047.64 g ± 145.74) and dark (L(*) = 70.02 ± 3.38) crumb bread, which was the least preferred by consumers. Results showed that tiger nut is a promising ingredient to formulate gluten-free baked products. PMID:24854294
Inskip, Chloe; Carter, Neil; Riley, Shawn; Roberts, Thomas; MacMillan, Douglas
Fostering local community tolerance for endangered carnivores, such as tigers (Panthera tigris), is a core component of many conservation strategies. Identification of antecedents of tolerance will facilitate the development of effective tolerance-building conservation action and secure local community support for, and involvement in, conservation initiatives. We use a stated preference approach for measuring tolerance, based on the 'Wildlife Stakeholder Acceptance Capacity' concept, to explore villagers' tolerance levels for tigers in the Bangladesh Sundarbans, an area where, at the time of the research, human-tiger conflict was severe. We apply structural equation modeling to test an a priori defined theoretical model of tolerance and identify the experiential and psychological basis of tolerance in this community. Our results indicate that beliefs about tigers and about the perceived current tiger population trend are predictors of tolerance for tigers. Positive beliefs about tigers and a belief that the tiger population is not currently increasing are both associated with greater stated tolerance for the species. Contrary to commonly-held notions, negative experiences with tigers do not directly affect tolerance levels; instead, their effect is mediated by villagers' beliefs about tigers and risk perceptions concerning human-tiger conflict incidents. These findings highlight a need to explore and understand the socio-psychological factors that encourage tolerance towards endangered species. Our research also demonstrates the applicability of this approach to tolerance research to a wide range of socio-economic and cultural contexts and reveals its capacity to enhance carnivore conservation efforts worldwide. PMID:26760035
Full Text Available Fostering local community tolerance for endangered carnivores, such as tigers (Panthera tigris, is a core component of many conservation strategies. Identification of antecedents of tolerance will facilitate the development of effective tolerance-building conservation action and secure local community support for, and involvement in, conservation initiatives. We use a stated preference approach for measuring tolerance, based on the 'Wildlife Stakeholder Acceptance Capacity' concept, to explore villagers' tolerance levels for tigers in the Bangladesh Sundarbans, an area where, at the time of the research, human-tiger conflict was severe. We apply structural equation modeling to test an a priori defined theoretical model of tolerance and identify the experiential and psychological basis of tolerance in this community. Our results indicate that beliefs about tigers and about the perceived current tiger population trend are predictors of tolerance for tigers. Positive beliefs about tigers and a belief that the tiger population is not currently increasing are both associated with greater stated tolerance for the species. Contrary to commonly-held notions, negative experiences with tigers do not directly affect tolerance levels; instead, their effect is mediated by villagers' beliefs about tigers and risk perceptions concerning human-tiger conflict incidents. These findings highlight a need to explore and understand the socio-psychological factors that encourage tolerance towards endangered species. Our research also demonstrates the applicability of this approach to tolerance research to a wide range of socio-economic and cultural contexts and reveals its capacity to enhance carnivore conservation efforts worldwide.
Full Text Available Abstract Background Bengal tiger Panthera tigris tigris the National Animal of India, is an endangered species. Estimating populations for such species is the main objective for designing conservation measures and for evaluating those that are already in place. Due to the tiger's cryptic and secretive behaviour, it is not possible to enumerate and monitor its populations through direct observations; instead indirect methods have always been used for studying tigers in the wild. DNA methods based on non-invasive sampling have not been attempted so far for tiger population studies in India. We describe here a pilot study using DNA extracted from faecal samples of tigers for the purpose of population estimation. Results In this study, PCR primers were developed based on tiger-specific variations in the mitochondrial cytochrome b for reliably identifying tiger faecal samples from those of sympatric carnivores. Microsatellite markers were developed for the identification of individual tigers with a sibling Probability of Identity of 0.005 that can distinguish even closely related individuals with 99.9% certainty. The effectiveness of using field-collected tiger faecal samples for DNA analysis was evaluated by sampling, identification and subsequently genotyping samples from two protected areas in southern India. Conclusion Our results demonstrate the feasibility of using tiger faecal matter as a potential source of DNA for population estimation of tigers in protected areas in India in addition to the methods currently in use.
Full Text Available The conversion of mangrove forest to aquaculture ponds has been increasing in recent decades. One of major concerns of this habitat loss is the release of stored 'blue' carbon from mangrove soils to the atmosphere. In this study, we assessed carbon dioxide (CO₂ efflux from soil in intensive shrimp ponds in Bali, Indonesia. We measured CO₂ efflux from the floors and walls of shrimp ponds. Rates of CO₂ efflux within shrimp ponds were 4.37 kg CO₂ m⁻² y⁻¹ from the walls and 1.60 kg CO₂ m⁻² y⁻¹ from the floors. Combining our findings with published data of aquaculture land use in Indonesia, we estimated that shrimp ponds in this region result in CO₂ emissions to the atmosphere between 5.76 and 13.95 Tg y⁻¹. The results indicate that conversion of mangrove forests to aquaculture ponds contributes to greenhouse gas emissions that are comparable to peat forest conversion to other land uses in Indonesia. Higher magnitudes of CO₂ emission may be released to atmosphere where ponds are constructed in newly cleared mangrove forests. This study indicates the need for incentives that can meet the target of aquaculture industry without expanding the converted mangrove areas, which will lead to increased CO₂ released to atmosphere.
Nadia Romero Salgado
Full Text Available Analysis of the effects of the shrimp industry development in the mangrove ecosystem, the socio-environmental conflicts generated and its relationship with neoliberalism and the financial crisis of 1999 in Ecuador. After a review of the importance of the mangrove ecosystem, the stages of the shrimp expansion, its promoters, the mangrove deforestation and the socio-environmental effects caused, I will analyze the shrimp crisis, its parallels with the financial crisis of 1999 and its subsequent recovery. I will show that the shrimp industry expanded in mangrove areas in order to reduce costs, even breaking the law and creating environmental degradation, vulnerability of the costs and loss of natural resources, based on the exploitation and privatization of a public good. This created unemployment, migration and impoverishment to local populations and costs that the State will have to assume. Therefore, it is a process of “accumulation by dispossession” characteristic of neoliberalism.
Versluis, Michel; Schmitz, Barbara; Heydt, von der Anna; Lohse, Detlef
The snapping shrimp (Alpheus heterochaelis) produces a loud snapping sound by an extremely rapid closure of its snapper claw. One of the effects of the snapping is to stun or kill prey animals. During the rapid snapper claw closure, a high-velocity water jet is emitted from the claw with a speed exc
Versluis, Michel; Heydt, von der Anna; Lohse, Detlef; Schmitz, Barbara
Snapping shrimp produce a snapping sound by an extremely rapid closure of their snapper claw. Source levels reported for Alpheus heterochaelis are as high as 220 dB (peak-to-peak) re. 1 µPa at 1 m distance. The loud snap has been attributed to the mechanical contact made when the snapper claw contra
Welleman, H.C.; Daan, N.
The fishery of the brown shrimp (Crangon crangon LINNEAUS 1758) is a widespread human activity in the coastal zone. Yet management of this fishery has never been implemented. The question is raised whether an uncontrolled fishery is sustainable or the conceivable ecological stress results in recruit
Dindo T. Ani
Full Text Available - Automatic system has brought many revolutions in the existing technologies. One among the technologies, which has greater developments, is the solar powered automatic shrimp feeding system. For instance, the solar power which is a renewable energy can be an alternative solution to energy crisis and basically reducing man power by using it in an automatic manner. The researchers believe an automatic shrimp feeding system may help solve problems on manual feeding operations. The project study aimed to design and develop a solar powered automatic shrimp feeding system. It specifically sought to prepare the design specifications of the project, to determine the methods of fabrication and assembly, and to test the response time of the automatic shrimp feeding system. The researchers designed and developed an automatic system which utilizes a 10 hour timer to be set in intervals preferred by the user and will undergo a continuous process. The magnetic contactor acts as a switch connected to the 10 hour timer which controls the activation or termination of electrical loads and powered by means of a solar panel outputting electrical power, and a rechargeable battery in electrical communication with the solar panel for storing the power. By undergoing through series of testing, the components of the modified system were proven functional and were operating within the desired output. It was recommended that the timer to be used should be tested to avoid malfunction and achieve the fully automatic system and that the system may be improved to handle changes in scope of the project.
Wenceslao Valenzuela Quiñónez
Full Text Available Shrimp aquaculture in Sinaloa is one of the top economic enterprises, generating many jobs and earns significant incomes every year. Shrimp feed is an essential part of maintaining healthy production. In this initial approach of shrimp growth in low salinity water, were tested two formulas of animal protein composed of 40% (APL1 and 20% (APL2 worm protein, a commercial diet, and no supplementary feed. Physicochemical parameters did not have a direct influence in shrimpbehavior. After six weeks of experimentation, shrimp fed with commercial diet had a weight gain 20% higher than those feed with worm protein. There were no significantly differences between sizes with respect to 40% animal protein and 20% animal protein with the commercial diet (P 0.05. However, shrimp fed worm protein had lower mortality. The use of worm protein could be an option to maintain a high quantity of shrimp reared in low salinity waters.
Spooner, B. S.; Metcalf, J.; DeBell, L.; Paulsen, A.; Noren, W.; Guikema, J. A.
Developmentally arrested brine shrimp cysts have been reactivated during orbital spaceflight on two different Space Shuttle missions (STS-50 and STS-54), and their subsequent development has been compared with that of simultaneously reactivated ground controls. Flight and control brine shrimp do not significantly differ with respect to hatching rates or larval morphology at the scanning and transmission EM levels. A small percentage of the flight larvae had defective nauplier eye development, but the observation was not statistically significant. However, in three different experiments on two different flights, involving a total of 232 larvae that developed in space, a highly significant difference in degree of flight to control development was found. By as early as 2.25 days after reactivation of development, spaceflight brine shrimp were accelerated, by a full instar, over ground control brine shrimp. Although developing more rapidly, flight shrimp grew as long as control shrimp at each developmental instar or stage.
Ashton, Elizabeth Clare
. Policy to position shrimp farms behind mangroves can be effective but also requires good institutional capacity and coordination, effective enforcement, incentives, land tenure and participation of all stakeholders for success. Better management practices have been identified which reduce impacts......Farmed shrimp production and value continue to increase with Asia producing the global majority of shrimp and the USA, Japan and Europe being the main importers. Shrimp farming systems are very diverse in their management, size and impacts. There are many causes for mangrove loss but the conversion...... of mangroves to shrimp farms has caused considerable attention. The major issues of shrimp farming include the loss of important ecological and socio-economic functions of mangrove ecosystems, changes in hydrology, salinization, introduction of non-native species and diseases, pollution from effluents...
Miller, Webb; Drautz, Daniela I; Janecka, Jan E; Lesk, Arthur M; Ratan, Aakrosh; Tomsho, Lynn P; Packard, Mike; Zhang, Yeting; McClellan, Lindsay R; Qi, Ji; Zhao, Fangqing; Gilbert, M Thomas P; Dalén, Love; Arsuaga, Juan Luis; Ericson, Per G P; Huson, Daniel H; Helgen, Kristofer M; Murphy, William J; Götherström, Anders; Schuster, Stephan C
We report the first two complete mitochondrial genome sequences of the thylacine (Thylacinus cynocephalus), or so-called Tasmanian tiger, extinct since 1936. The thylacine's phylogenetic position within australidelphian marsupials has long been debated, and here we provide strong support for the...
Gopalaswamy, Arjun M.; Royle, J. Andrew; Delampady, Mohan; Nichols, James D.; Karanth, K. Ullas; Macdonald, David W.
A productive way forward in studies of animal populations is to efficiently make use of all the information available, either as raw data or as published sources, on critical parameters of interest. In this study, we demonstrate two approaches to the use of multiple sources of information on a parameter of fundamental interest to ecologists: animal density. The first approach produces estimates simultaneously from two different sources of data. The second approach was developed for situations in which initial data collection and analysis are followed up by subsequent data collection and prior knowledge is updated with new data using a stepwise process. Both approaches are used to estimate density of a rare and elusive predator, the tiger, by combining photographic and fecal DNA spatial capture–recapture data. The model, which combined information, provided the most precise estimate of density (8.5 ± 1.95 tigers/100 km2 [posterior mean ± SD]) relative to a model that utilized only one data source (photographic, 12.02 ± 3.02 tigers/100 km2 and fecal DNA, 6.65 ± 2.37 tigers/100 km2). Our study demonstrates that, by accounting for multiple sources of available information, estimates of animal density can be significantly improved.
Diket, Read M.; Brewer, Thomas M.
This paper grabs hold of the "assessment tiger" by considering the history of the National Assessment of Educational Progress (NAEP) "Arts Report Cards" for the visual arts, which were constructed and have been administered four times within thirty-five years. Two purposes of the NAEP have persisted since its founding: (1) measuring learning and…
Keawcharoen, Juthatip; Oraveerakul, Kanisak; Kuiken, Thijs; Fouchier, Ron A M; Amonsin, Alongkorn; Payungporn, Sunchai; Noppornpanth, Suwanna; Wattanodorn, Sumitra; Theamboonlers, Apiradee; Tantilertcharoen, Rachod; Pattanarangsan, Rattapan; Arya, Nlin; Ratanakorn, Parntep; Osterhaus, Albert D. M. E.; Poovorawan, Yong
Influenza virus is not known to affect wild felids. We demonstrate that avian influenza A (H5N1) virus caused severe pneumonia in tigers and leopards that fed on infected poultry carcasses. This finding extends the host range of influenza virus and has implications for influenza virus epidemiology and wildlife conservation.
Full Text Available The Sumatran tiger (Panthera tigris sumatrae is one of several endemic Indonesian wild cat groups which population is critically endangered. A program to increase the population size had been conducted in captivity, especially in the zoo. In order to monitor the captive population and for the means of management in captivity, a logbook data recording system had been developed for individual animals. A compilation data from the Tiger International Stud Book from 1942 to 2000 was analyzed. The extraction data consisted of the reproduction performance of the animals, such as calving pattern, sex ratio, litter size etc. The results showed that mortality of cubs at ≤ 5 months old reached 59%, between 5 and 24 months old was 9.3% and above 24 months was 31.7%. Cubs were born all year round with concentration in July for Europe and North America regions. The mean of first reproductive age was at 4.6 years old (± 2.28, with the mean of the oldest reproductive age was at 8.3 years (± 3.63. Mean litter size was 2.21 cubs from dame born in captivity and 2.45 cubs from dame capture from the wild. Sex ratio of male to female was 53.8:46.2. The average lifespan of adult wild captive tiger was 5108.9 day (± 2365.4 day, while for adult (≥ 24 months of age captive tiger was 4417.4 day (± 1972.7.
This draft report documents the Tiger Team Assessment of the Fermi National Accelerator Laboratory (Fermilab) located in Batavia, Illinois. Fermilab is a program-dedicated national laboratory managed by the Universities Research Association, Inc. (URA) for the US Department of Energy (DOE). The Tiger Team Assessment was conducted from May 11 to June 8, 1992, under the auspices of DOE's Office of Special Projects (OSP) under the Office of the Assistant Secretary for Environment, Safety and Health (EH). The assessment was comprehensive, encompassing environmental, safety and health (ES ampersand H), and quality assurance (QA) disciplines; site remediation; facilities management; and waste management operations. Compliance with applicable Federal , State of Illinois, and local regulations; applicable DOE Orders; best management practices; and internal Fermilab requirements was addressed. In addition, an evaluation of the effectiveness of DOE and Fermilab management of the ES ampersand H/QA and self-assessment programs was conducted. The Fermilab Tiger Team Assessment is part a larger, comprehensive DOE Tiger Team Independent Assessment Program planned for DOE facilities. The objective of the initiative is to provide the Secretary of Energy with information on the compliance status of DOE facilities with regard to ES ampersand H requirements, root causes for noncompliance, adequacy of DOE and contractor ES ampersand H management programs, response actions to address the identified problem areas, and DOE-wide ES ampersand H compliance trends and root causes
Niederjohn, M. Scott; Schug, Mark C.; Wood, William C.
"One who rides a tiger is afraid to dismount," says a colorful proverb from an earlier time. This may be an apt saying for the situation facing the new head of the Federal Reserve, Janet L. Yellen, who takes over at a time when successive rounds of Fed policy have taken the central bank into uncharted territory. By historical standards,…
Beardsley, Christine; Moss, Shaun; Malfatti, Francesca; Azam, Farooq
Microorganisms play integral roles in the cycling of carbon (C) and nitrogen (N) in recirculating aquaculture systems (RAS) for fish and shellfish production. We quantified the pathways of shrimp fecal bacterial activities and their role in C- and N-flux partitioning relevant to culturing Pacific white shrimp, Penaeus (Litopenaeus) vannamei, in RAS. Freshly produced feces from P. vannamei contained 0.6-7 × 10(10) bacteria g(-1) dry wt belonging to Bacteroidetes (7%), Alphaproteobacteria (4%), and, within the Gammaproteobacteria, almost exclusively to the genus Vibrio (61%). Because of partial disintegration of the feces (up to 27% within 12 h), the experimental seawater became inoculated with fecal bacteria. Bacteria grew rapidly in the feces and in the seawater, and exhibited high levels of aminopeptidase, chitinase, chitobiase, alkaline phosphatase, α- and β-glucosidase, and lipase activities. Moreover, fecal bacteria enriched the protein content of the feces within 12 h, potentially enriching the feces for the coprophagous shrimp. The bacterial turnover time was much faster in feces (1-10 h) than in mature RAS water (350 h). Thus, shrimp fecal bacteria not only inoculate RAS water but also contribute to bacterial abundance and productivity, and regulate system processes important for shrimp health. PMID:21426366
Naha, Dipanjan; Jhala, Yadvendradev V; Qureshi, Qamar; Roy, Manjari; Sankar, Kalyansundaram; Gopal, Rajesh
The Sundarban of India and Bangladesh (about 6000 km²) are the only mangrove forests inhabited by a sizeable population of tigers. The adjoining area also supports one of the highest human densities and experiences severe human-tiger conflicts. We used GPS-Satellite and VHF radio-collars on 6 (3 males and 3 female) tigers to study their ranging patterns and habitat preference. The average home range (95% Fixed Kernel) for resident females was 56.4 (SE 5.69) and for males it was 110 (SE 49) km². Tigers crossed an average of 5 water channels > 30 meters per day with a mean width of 54 meters, whereas channels larger than 400 meters were rarely crossed. Tigers spent over 58% of their time within Phoenix habitat but compositional analysis showed a habitat preference of the order Avicennia-Sonneratia > Phoenix > Ceriops > Barren > Water. Average daily distance moved was 4.6 km (range 0.1-23). Activity of tigers peaked between 05:00 hours and 10:00 hours showing some overlap with human activity. Territory boundaries were demarcated by large channels which tigers intensively patrolled. Extra caution should be taken while fishing or honey collection during early morning in Avicennia-Sonneratia and Phoenix habitat types along wide channels to reduce human-tiger conflict. Considering home-range core areas as exclusive, tiger density was estimated at 4.6 (SE range 3.6 to 6.7) tigers/100 km2 giving a total population of 76 (SE range 59-110) tigers in the Indian Sundarban. Reluctance of tigers to cross wide water channels combined with increasing commercial boat traffic and sea level rise due to climate change pose a real threat of fragmenting the Sundarban tiger population. PMID:27049644
Naha, Dipanjan; Jhala, Yadvendradev V.; Qureshi, Qamar; Roy, Manjari; Sankar, Kalyansundaram; Gopal, Rajesh
The Sundarban of India and Bangladesh (about 6000 km²) are the only mangrove forests inhabited by a sizeable population of tigers. The adjoining area also supports one of the highest human densities and experiences severe human-tiger conflicts. We used GPS-Satellite and VHF radio-collars on 6 (3 males and 3 female) tigers to study their ranging patterns and habitat preference. The average home range (95% Fixed Kernel) for resident females was 56.4 (SE 5.69) and for males it was 110 (SE 49) km². Tigers crossed an average of 5 water channels > 30 meters per day with a mean width of 54 meters, whereas channels larger than 400 meters were rarely crossed. Tigers spent over 58% of their time within Phoenix habitat but compositional analysis showed a habitat preference of the order Avicennia-Sonneratia > Phoenix > Ceriops > Barren > Water. Average daily distance moved was 4.6 km (range 0.1–23). Activity of tigers peaked between 05:00 hours and 10:00 hours showing some overlap with human activity. Territory boundaries were demarcated by large channels which tigers intensively patrolled. Extra caution should be taken while fishing or honey collection during early morning in Avicennia-Sonneratia and Phoenix habitat types along wide channels to reduce human-tiger conflict. Considering home-range core areas as exclusive, tiger density was estimated at 4.6 (SE range 3.6 to 6.7) tigers/100 km2 giving a total population of 76 (SE range 59–110) tigers in the Indian Sundarban. Reluctance of tigers to cross wide water channels combined with increasing commercial boat traffic and sea level rise due to climate change pose a real threat of fragmenting the Sundarban tiger population. PMID:27049644
Wenceslao Valenzuela Quiñónez; Gerardo Rodríguez-Quiroz; Héctor Manuel Esparza Leal; Eusebio Nava Pérez
Shrimp aquaculture in Sinaloa is one of the top economic enterprises, generating many jobs and earns significant incomes every year. Shrimp feed is an essential part of maintaining healthy production. In this initial approach of shrimp growth in low salinity water, were tested two formulas of animal protein composed of 40% (APL1) and 20% (APL2) worm protein, a commercial diet, and no supplementary feed. Physicochemical parameters did not have a direct influence in shrimpbehavior. After six we...
Gholamreza jahed Khaniki; Parisa Sadighara; Ramin Nabizadeh Nodehi; Mahmood Alimohammadi; Naiema Vakili Saatloo
Objective: To find effective method for carotenoids extraction from shrimp waste which is one of the important sources of natural carotenoids and produced in large quantities in Iran. Methods: Two methods of carotenoids extraction, enzymatic and alkaline (NaOH 1 normal) treatment, were assayed. About 5 g of gritted shrimp wastes were used at each stage. For alkaline treatment, sodium hydroxide were added to shrimp waste. After 48 h, the mixture was filtered and centrifuged. ...
White spot syndrome virus (WSSV) is the most important viral pathogen of cultured penaeid shrimp worldwide. Since the initial discovery of the virus inTaiwanin 1992, it has spread to shrimp farming regions in Southeast Asia, theAmericas, Europe and theMiddle Eastcausing major economic losses. The virus has a wide host range among crustaceans and induces distinctive clinical signs (white spots)on the inner surface of the exoskeletonof penaeid shrimps.Limited data is available about the immune ...
Feed palatability in carnivorous aquaculture species, shrimps in particular, has been crucially related to the presence of compounds acting as attractants that are commonly associated with the prey components under wild conditions. Thus a nutritionally adequate and organoleptically-pleasing diet is essential to achieve satisfactory intake and growth in shrimps. Historically, fishmeal has been an essential dietary component of intensive shrimp cultures because of its nutrient composit...
Anas M. Fauzi; R.Dikky Indrawan; Alim Setiawan Slamet; Farida Ratna Dewi; Lindawati Kartika; Firmansjah S
Kampung Vannamei as shrimp cluster is being developed since 2004 by PT CP Prima, tbk Surabaya through Shrimp Culture Health Management transformation technology to several traditional farmers in Gresik, Lamongan, Tuban, and Madura areas. The research objectives aims to identify and mapping of stakeholder, to analyze interaction of stakeholders, to formulate strategy from internal and external environment factors and to set priority on strategy to develop sustainable and competitive shrimp clu...
Aaron J. CORCORAN, William E. CONNER, Jesse R. BARBER
Full Text Available The night sky is the venue of an ancient acoustic battle between echolocating bats and their insect prey. Many tiger moths (Lepidoptera: Arctiidae answer the attack calls of bats with a barrage of high frequency clicks. Some moth species use these clicks for acoustic aposematism and mimicry, and others for sonar jamming, however, most of the work on these defensive functions has been done on individual moth species. We here analyze the diversity of structure in tiger moth sounds from 26 species collected at three locations in North and South America. A principal components analysis of the anti-bat tiger moth sounds reveals that they vary markedly along three axes: (1 frequency, (2 duty cycle (sound production per unit time and frequency modulation, and (3 modulation cycle (clicks produced during flexion and relaxation of the sound producing tymbal structure. Tiger moth species appear to cluster into two distinct groups: one with low duty cycle and few clicks per modulation cycle that supports an acoustic aposematism function, and a second with high duty cycle and many clicks per modulation cycle that is consistent with a sonar jamming function. This is the first evidence from a community-level analysis to support multiple functions for tiger moth sounds. We also provide evidence supporting an evolutionary history for the development of these strategies. Furthermore, cross-correlation and spectrogram correlation measurements failed to support a “phantom echo” mechanism underlying sonar jamming, and instead point towards echo interference [Current Zoology 56 (3: 358–369, 2010].
Karanth, K.U.; Nichols, J.D.; Kumar, N.S.; Link, W.A.; Hines, J.E.
The goal of ecology is to understand interactions that determine the distribution and abundance of organisms. In principle, ecologists should be able to identify a small number of limiting resources for a species of interest, estimate densities of these resources at different locations across the landscape, and then use these estimates to predict the density of the focal species at these locations. In practice, however, development of functional relationships between abundances of species and their resources has proven extremely difficult, and examples of such predictive ability are very rare. Ecological studies of prey requirements of tigers Panthera tigris led us to develop a simple mechanistic model for predicting tiger density as a function of prey density. We tested our model using data from a landscape-scale long-term (1995-2003) field study that estimated tiger and prey densities in 11 ecologically diverse sites across India. We used field techniques and analytical methods that specifically addressed sampling and detectability, two issues that frequently present problems in macroecological studies of animal populations. Estimated densities of ungulate prey ranged between 5.3 and 63.8 animals per km2. Estimated tiger densities (3.2-16.8 tigers per 100 km2) were reasonably consistent with model predictions. The results provide evidence of a functional relationship between abundances of large carnivores and their prey under a wide range of ecological conditions. In addition to generating important insights into carnivore ecology and conservation, the study provides a potentially useful model for the rigorous conduct of macroecological science.
The results of supplementing crustacean feeds with vitamins are examined specifically from the standpoint of shrimp culture. Micro-nutrients selected for discussion include: water-soluble vitamins of the B-complex, choline and inositol, vitamin C and the fat-soluble group of vitamins: A, D, E and K. Ways in which utilization of vitamins and ultimately dietary demand are altered by physiological state, conditions of culture, as well as factors which impact on feed levels, are explored.
De Grave, Sammy; Smith, Kevin G; Adeler, Nils A; Allen, Dave J; Alvarez, Fernando; Anker, Arthur; Cai, Yixiong; Carrizo, Savrina F; Klotz, Werner; Mantelatto, Fernando L; Page, Timothy J; Shy, Jhy-Yun; Villalobos, José Luis; Wowor, Daisy
We present the first global assessment of extinction risk for a major group of freshwater invertebrates, caridean shrimps. The risk of extinction for all 763 species was assessed using the IUCN Red List criteria that include geographic ranges, habitats, ecology and past and present threats. The Indo-Malayan region holds over half of global species diversity, with a peak in Indo-China and southern China. Shrimps primarily inhabit flowing water; however, a significant subterranean component is present, which is more threatened than the surface fauna. Two species are extinct with a further 10 possibly extinct, and almost one third of species are either threatened or Near Threatened (NT). Threats to freshwater shrimps include agricultural and urban pollution impact over two-thirds of threatened and NT species. Invasive species and climate change have the greatest overall impact of all threats (based on combined timing, scope and severity of threats). PMID:25807292
Sammy De Grave
Full Text Available We present the first global assessment of extinction risk for a major group of freshwater invertebrates, caridean shrimps. The risk of extinction for all 763 species was assessed using the IUCN Red List criteria that include geographic ranges, habitats, ecology and past and present threats. The Indo-Malayan region holds over half of global species diversity, with a peak in Indo-China and southern China. Shrimps primarily inhabit flowing water; however, a significant subterranean component is present, which is more threatened than the surface fauna. Two species are extinct with a further 10 possibly extinct, and almost one third of species are either threatened or Near Threatened (NT. Threats to freshwater shrimps include agricultural and urban pollution impact over two-thirds of threatened and NT species. Invasive species and climate change have the greatest overall impact of all threats (based on combined timing, scope and severity of threats.
In May 2007, investigators discovered that waste material from the pet food manufacturing process contaminated with melamine (MEL) and/or cyanuric acid (CYA) had been added to hog and chicken feeds. At this time, investigators also learned that adulterated wheat gluten had been used in the manufacture of aquaculture feeds. Concern that the contaminated feed had been used in aquaculture and could enter the human food supply prompted the development of a method for the determination of CYA residues in the edible tissues of fish and shrimp. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed as a sensitive technique for the analysis of CYA in catfish, tilapia, salmon, trout and shrimp tissue. CYA was extracted from ground fish or shrimp with an acetic acid solution, defatted with hexane, and isolated with a graphitic carbon black solid-phase extraction column. Residues were separated from matrix components using a porous graphitic carbon LC column, and then analyzed with electrospray ionization in negative ion mode on a triple quadrupole mass spectrometer. Selective reaction monitoring was performed on the [M-H]-m/z 128 ion resulting in the product ions m/z 85 and 42. Recoveries from catfish, tilapia and trout fortified with 10-100 μg kg-1 of CYA averaged 67% with a relative standard deviation (R.S.D.) of 18% (n = 107). The average method detection limit (MDL) for catfish, tilapia and trout is 3.5 μg kg-1. An internal standard, 13C3-labeled CYA, was used in the salmon and shrimp extractions. Average recovery of CYA from salmon was 91% (R.S.D. = 15%, n = 18) with an MDL of 7.4 μg kg-1. Average recovery of CYA from shrimp was 85% (R.S.D. = 10%, n = 13) with an MDL of 3.5 μg kg-1
Full Text Available Cell surface display using the yeasts Saccharomyces cerevisiae and Pichia pastoris has been extensively developed for application in bioindustrial processes. Due to the rigid structure of their cell walls, a number of proteins have been successfully displayed on their cell surfaces. It was previously reported that the viral binding protein Rab7 from the giant tiger shrimp Penaeus monodon (PmRab7 and its binding partner envelope protein VP28 of white spot syndrome virus (WSSV could independently protect shrimp against WSSV infection. Thus, we aimed to display these two proteins independently on the cell surfaces of 2 yeast clones with the ultimate goal of using a mixture of the two clones as an orally deliverable, antiviral agent to protect shrimp against WSSV infection. PmRab7 and VP28 were modified by N-terminal tagging to the C-terminal half of S. cerevisiae α-agglutinin. DNA fragments, harboring fused-gene expression cassettes under control of an alcohol oxidase I (AOX1 promoter were constructed and used to transform the yeast cells. Immunofluorescence microscopy with antibodies specific to both proteins demonstrated that mutated PmRab7 (mPmRab7 and partial VP28 (pVP28 were localized on the cell surfaces of the respective clones, and fluorescence intensity for each was significantly higher than that of control cells by flow cytometry. Enzyme-linked immunosorbant assay (ELISA using cells displaying mPmRab7 or pVP28 revealed that the binding of specific antibodies for each was dose-dependent, and could be saturated. In addition, the binding of mPmRab7-expressing cells with free VP28, and vice versa was dose dependent. Binding between the two surface-expressed proteins was confirmed by an assay showing agglutination between cells expressing complementary mPmRab7 and pVP28. In summary, our genetically engineered P. pastoris can display biologically active mPmRab7 and pVP28 and is now ready for evaluation of efficacy in protecting shrimp against
Li, Xianfeng; Snyder, James A.; Stuart, Steven J.; Latour, Robert A.
The recently developed "temperature intervals with global exchange of replicas" (TIGER2) accelerated sampling method is found to have inaccuracies when applied to systems with explicit solvation. This inaccuracy is due to the energy fluctuations of the solvent, which cause the sampling method to be less sensitive to the energy fluctuations of the solute. In the present work, the problem of the TIGER2 method is addressed in detail and a modification to the sampling method is introduced to correct this problem. The modified method is called "TIGER2 with solvent energy averaging," or TIGER2A. This new method overcomes the sampling problem with the TIGER2 algorithm and is able to closely approximate Boltzmann-weighted sampling of molecular systems with explicit solvation. The difference in performance between the TIGER2 and TIGER2A methods is demonstrated by comparing them against analytical results for simple one-dimensional models, against replica exchange molecular dynamics (REMD) simulations for sampling the conformation of alanine dipeptide and the folding behavior of (AAQAA)3 peptide in aqueous solution, and by comparing their performance in sampling the behavior of hen egg-white lysozyme in aqueous solution. The new TIGER2A method solves the problem caused by solvent energy fluctuations in TIGER2 while maintaining the two important characteristics of TIGER2, i.e., (1) using multiple replicas sampled at different temperature levels to help systems efficiently escape from local potential energy minima and (2) enabling the number of replicas used for a simulation to be independent of the size of the molecular system, thus providing an accelerated sampling method that can be used to efficiently sample systems considered too large for the application of conventional temperature REMD.
A black ring is a five-dimensional black hole with an event horizon of topology S1 x S2. We provide an introduction to the description of black rings in general relativity and string theory. Novel aspects of the presentation include a new approach to constructing black ring coordinates and a critical review of black ring microscopics. (topical review)
This paper deals with a new system of aquaculture, i.e., a closed recirculating system for shrimp-mollusk polyculture. The culture system consisted of several shrimp ponds, a mollusk water-purifying pond and a reservoir. During the production cycle, water circulated between the shrimp and mollusk ponds, and the reservoir compensated for water loss from seepage and evaporation. Constricted tagelus, Sinonovacula constricta, was selected as the cultured mollusk, and Pacific white shrimp, Litopenaeus vannamei, as the cultured shrimp. The main managing measures during the production cycle were: setting and using the aerators; introducing the probiotic products timely into the shrimp ponds; adopting a "pen-closing" method for controlling shrimp viral epidemics; setting the flow diversion barriers in the mollusk pond to keep the circulating water flowing through the pond along a sine-like curve and serve as substrate for biofilm; no direct feeding was necessary for the cultured mollusk until the co-cultured shrimp was harvested; natural foods in the water from the shrimp ponds was used for their foods. Two sets of the system were used in the experiment in 2002 and satisfactory results were achieved. The average yield of the shrimp was 11 943.5 kg/hm2, and that of the mollusk was 16 965kg/hm2. After converting the mollusk yield into shrimp yield at their market price ratio, the food coefficient of the entire system averaged at as low as 0.81. The water quality in the ponds was maintained at a desirable level and no viral epidemics were discovered during the production cycle.
Wu, Xiongfei; Zhao, Zhidong; Li, Deshang; Chang, Kangmei; Tong, Zhuanshang; Si, Liegang; Xu, Kaichong; Ge, Bailin
This paper deals with a new system of aquaculture, i.e., a closed recirculating system for shrimp-mollusk polyculture. The culture system consisted of several shrimp ponds, a mollusk water-purifying pond and a reservoir. During the production cycle, water circulated between the shrimp and mollusk ponds, and the reservoir compensated for water loss from seepage and evaporation. Constricted tagelus, Sinonovacula constricta, was selected as the cultured mollusk, and Pacific white shrimp, Litopenaeus vannamei, as the cultured shrimp. The main managing measures during the production cycle were: setting and using the aerators; introducting the probiotic products timely into the shrimp ponds; adopting a “pen-closing” method for controlling shrimp viral epidemics; setting the flow diversion barriers in the mollusk pond to keep the circulating water flowing through the pond along a sine-like curve and serve as substrate for biofilm; no direct feeding was necessary for the cultured mollusk until the co-cultured shrimp was harvested; natural foods in the water from the shrimp ponds was used for their foods. Two sets of the system were used in the experiment in 2002 and satisfactory results were achieved. The average yield of the shrimp was 11 943.5 kg/hm2, and that of the mollusk was 16 965 kg/hm2. After converting the mollusk yield into shrimp yield at their market price ratio, the food coefficient of the entire system averaged at as low as 0.81. The water quality in the ponds was maintained at a desirable level and no viral epidemics were discovered during the production cycle.
Singh Lalji; Bhagavatula Jyotsna
Abstract Background Bengal tiger Panthera tigris tigris the National Animal of India, is an endangered species. Estimating populations for such species is the main objective for designing conservation measures and for evaluating those that are already in place. Due to the tiger's cryptic and secretive behaviour, it is not possible to enumerate and monitor its populations through direct observations; instead indirect methods have always been used for studying tigers in the wild. DNA methods ba...
U. Farooq*, S. Sajjad1, M. Anwar1 and B.N. Khan2
There is a dearth of published literature regarding the effect of captivity on serum chemistry variables of tigers kept in the zoos and wildlife sanctuaries. The present study was hence conducted to determine and compare serum chemistry values in tigers of Bengal origin (Panthera tigris tigris) kept in captivity at Lahore zoo (LZ) (n=4) and in semi natural environment of Lahore Wildlife Park (LWP) (n=6), Pakistan. The tigers kept at LZ had significantly (P
Singh, Sujeet Kumar; Mishra, Sudhanshu; Aspi, Jouni; Kvist, Laura; Nigam, Parag; Pandey, Puneet; Sharma, Reeta; Goyal, Surendra Prakash
The Sundarbans tiger inhabits a unique mangrove habitat and are morphologically distinct from the recognized tiger subspecies in terms of skull morphometrics and body size. Thus, there is an urgent need to assess their ecological and genetic distinctiveness and determine if Sundarbans tigers should be defined and managed as separate conservation unit. We utilized nine microsatellites and 3 kb from four mitochondrial DNA (mtDNA) genes to estimate genetic variability, population structure, demo...
Mu-Yeong Lee; Jee Yun Hyun; Seo-Jin Lee; Junghwa An; Eunok Lee; Mi-Sook Min; Junpei Kimura; Shin-ichiro Kawada; Nozomi Kurihara; Shu-Jin Luo; Stephen J. O’Brien; Hang Lee
The tiger population that once inhabited the Korean peninsula was initially considered a unique subspecies (Panthera tigris coreensis), distinct from the Amur tiger of the Russian Far East (P. t. altaica). However, in the following decades, the population of P. t. coreensis was classified as P. t. altaica and hence forth the two populations have been considered the same subspecies. From an ecological point of view, the classification of the Korean tiger population as P. t. altaica is a plausi...
Joshi, Aditya; Vaidyanathan, Srinivas; Mondol, Samrat; Edgaonkar, Advait; Ramakrishnan, Uma
Today, most wild tigers live in small, isolated Protected Areas within human dominated landscapes in the Indian subcontinent. Future survival of tigers depends on increasing local population size, as well as maintaining connectivity between populations. While significant conservation effort has been invested in increasing tiger population size, few initiatives have focused on landscape-level connectivity and on understanding the effect different landscape elements have on maintaining connecti...
Muraree Lal Meena
It is well known that Sariska Tiger Reserve is a home to the India’s national animal - the Bengal Tiger (Panthera tigris tigris). The crux of this research is to examine the role of local peoples in the conservation of Sariska Tiger project, which was declared a wildlife reserve way back in 1955 and then further raised to a status of a Tiger Reserve in 1978, and a National Park in 1982. According to the Government officials, the people around the reserve are not only responsible for degrading...
A number of parvoviruses are now know to infect marine shrimp, and these viruses alone or in combination with other viruses have the potential to cause major losses in shrimp aquaculture globally. This review provides a comprehensive overview of the biology, genome organization, gene expression, and...
齐振雄; 李德尚; 张曼平; 董双林
April to October, 1997 comparative studios on the nitrogen budgets of closed shrimp polyculture systems showed that, in all the studied polyculture systems, nitrogen from feeds and fertilizers were the main input items, which comprised 70.7% - 83.9% of the total input nitrogen, 3.2% - 7.4% of which was provided by nitrogen fixation. It was in monoculture enclosures (Y-4, Y-11 and Y-12) that the percentage reached the maximum value. The output nitrogen in harvested products comprised 10.8% - 24.6% of total input nitrogen, and the highest percentage, 24.6%, was found in shrimp-fish-tagelus polyculture systems. In shrimp monoculture and shrimp-fish polyculture systems, they were 19.1% and 21.9%, respectively. The nitrogen utilization efficiency was different and varied from 12.2% to 20.1%. The highest, 20.1%, was found in shrimp-fish-tagelus polycultttre systems, and the average of 20.0% was found in shrimp-tagelus polyculture systems. The lowest, 12.2%, was found in shrimp monoculture systems.All the nitrogen utilization efficiencies in shrimp-fish systems or shrimp-scallop systems seemed to be higher than that of the monoculture system, but they showed little statistical difference. The main outputs of nitrngen were found in sediment mud, and comprised 48.2% -60.8% of the total input, the lowest percentage was found in shrimp-fish-tagelus polyculture systems, and the highost percentage in shrimp-scallop systems.During the experiment, nitrogen lost through denitrification and ammonia volatilization comprised 1.9% - 6.2%, averaged 2.8%, of the total input, and the loss through seepage comprised 5.9% - 8.9% of the total.The estimated nitrogen attached to the enclosure wall comprised 3.7% - 13.3% of the total, and was highest in shrimp monoculture systems. Compared with the classic shrimp farming industry, the closed shrimp polycul-ture systems may improve the nitrogen utilization efficiency, and hence reduce the environmental impacts on coastal waters. The nitrogen
April to October, 1997 comparative studies on the nitrogen budgets of closed shrimp polyculture systems showed that, in all the studied polyculture systems, nitrogen from feeds and fertilizers were the main input items, which comprised 70.7%-83.9% of the total input nitrogen, 3.2%-7.4% of which was provided by nitrogen fixation. It was in monoculture enclosures (Y-4, Y-11 and Y-12) that the percentage reached the maximum value. The output nitrogen in harvested products comprised 10.8%-24.6% of total input nitrogen, and the highest percentage, 24.6%, was found in shrimp-fish-tagelus polyculture systems. In shrimp monoculture and shrimp-fish polyculture systems, they were 19.1% and 21.9%, respectively. The nitrogen utilization efficiency was different and varied from 12.2% to 20.1%. The highest, 20.1%, was found in shrimp-fish-tagelus polyculture systems, and the average of 20.0% was found in shrimp-tagelus polyculture systems. The lowest, 12.2%, was found in shrimp monoculture systems. All the nitrogen utilization efficiencies in shrimp-fish systems or shrimp-scallop systems seemed to be higher than that of the monoculture system, but they showed little statistical difference. The main outputs of nitrogen were found in sediment mud, and comprised 48.2%-60.8% of the total input, the lowest percentage was found in shrimp-fish-tagelus polyculture systems, and the highest percentage in shrimp-scallop systems. During the experiment, nitrogen lost through denitrification and ammonia volatilization comprised 1.9%-6.2%, averaged 2.8%, of the total input, and the loss through seepage comprised 5.9%-8.9% of the total. The estimated nitrogen attached to the enclosure wall comprised 3.7%-13.3% of the total, and was highest in shrimp monoculture systems. Compared with the classic shrimp farming industry, the closed shrimp polyculture systems may improve the nitrogen utilization efficiency, and hence reduce the environmental impacts on coastal waters. The nitrogen discharging
By reducing the number of bacteria, irradiation with a dose of 3 kGy increased the shelf life of dried and salted shrimp. Combining radiation with low-temperature storage further increased shelf life. Vacuum packaging the shrimp in polyethylene-laminated polyethylene bags resulted in extension of the shelf life by more than 15 weeks. Packing in tupperware and polyethylene bags was less effective. Most of the bacteria in the dried and salted shrimps were halophiles. Salmonellae and coliforms were not detected in the samples studied. During storage, ammonia was formed in the shrimps. Irradiation caused less ammonia to be formed. Low-temperature storage also reduced ammonia formation. The sensory quality of the shrimps decreased with increasing amounts of ammonia. (author). 9 refs, 25 figs
Flaherty, Mark; Karnjanakesorn, Choomjet
Rising demand for shrimp in the developed nations has helped to foster a dramatic growth in marine shrimp aquaculture, particularly in South America and South Asia. In Thailand, Marine shrimp aquaculture is now an important earmer of foreign exchange. The growth in Production has been achieved through the expansion of the culture area and the adoption of intensive production methods. The conversion of near-shore areas to shrimp culture, however, is proving to have many consequences that impinge on the environmental integrity of coastal areas. This paper reviews the development of Thailand's marine shrimp culture industry and examines the nature of the environmental impacts that are emerging. It then discusses the implications these have for rural poor and the long-term viability of the culture industry.
You can set your watch to it: As soon as Apple comes out with another version of Mac OS X, David Pogue hits the streets with another meticulous Missing Manual to cover it with a wealth of detail. The new Mac OS X 10.4, better known as Tiger, is faster than its predecessors, but nothing's too fast for Pogue and Mac OS X: The Missing Manual. There are many reasons why this is the most popular computer book of all time. With its hallmark objectivity, the Tiger Edition thoroughly explores the latest features to grace the Mac OS. Which ones work well and which do not? What should you look for? Th
This draft document contains findings identified during the Tiger Team Compliance Assessment of the US Department of Energy Savannah River Site (SRS), located in three countries (Aiken, Barnwell and Allendale), South Carolina. The Assessment was directed by the Department's Office of the Assistant Secretary for Environment, Safety, and Health (ES ampersand H) and was conducted from January 29 to March 23, 1990. The Savannah River Site Tiger Team Compliance Assessment was broad in scope covering the Environment, Safety and Health, and Management areas and was designed to determine the site's compliance with applicable Federal (including DOE), state, and local regulations and requirements. The scope of the Environmental assessment was sitewide while the Safety and Health assessments included site operating facilities (except reactors), and the sitewide elements of Aviation Safety, Emergency Preparedness, Medical Services, and Packaging and Transportation. This report contains the appendices to the assessment
This report documents the Tiger Team Assessment of the Naval Petroleum Reserves in California (NPRC) which consists of Naval Petroleum Reserve Number 1 (NPR-1), referred to as the Elk Hills oil field and Naval Petroleum Reserve Number 2 (NPR-2), referred to as the Buena Vista oil field, each located near Bakersfield, California. The Tiger Team Assessment was conducted from November 12 to December 13, 1991, under the auspices of DOE's Office of Special Projects (OSP) under the Assistant Secretary for Environment, Safety and Health (EH). The assessment was comprehensive, encompassing environmental, safety, and health (ES ampersand H), and quality assurance (OA) disciplines; site remediation; facilities management; and waste management operations. Compliance with applicable Federal, State of California, and local regulations; applicable DOE Orders; best management practices; and internal NPRC requirements was assessed. In addition, an evaluation of the adequacy and effectiveness of DOE/NPRC, CUSA, and BPOI management of the ES ampersand H/QA programs was conducted
This report documents the Tiger Team Assessment of the Los Alamos National Laboratory (LANL) located in Los Alamos, New Mexico. LANL is operated for the US Department of Energy (DOE) by the University of California. The Tiger Team Assessment was conducted from September 23 to November 8, 1991, under the auspices of the DOE Office of Special Projects, Office of Assistant Secretary for Environment, Safety and Health. The assessment was comprehensive, encompassing environmental, safety, and health (ES H) disciplines; management; and contractor and DOE self-assessments. Compliance with applicable Federal, state, and local regulations; applicable DOE Orders; best management practices; and internal LANL site requirements was assessed. In addition, an evaluation of the adequacy and effectiveness of the DOE and the site contractors' management of ES H/quality assurance programs was conducted. This volume discusses findings concerning the environmental assessment.
This report documents the Tiger Team Assessment of the Los Alamos National Laboratory (LANL) located in Los Alamos, New Mexico. LANL is operated for the US Department of Energy (DOE) by the University of California. The Tiger Team Assessment was conducted from September 23 to November 8, 1991, under the auspices of the DOE Office of Special Projects, Office of Assistant Secretary for Environment, Safety and Health. The assessment was comprehensive, encompassing environmental, safety, and health (ES ampersand H) disciplines; management; and contractor and DOE self-assessments. Compliance with applicable Federal, state, and local regulations; applicable DOE Orders; best management practices; and internal LANL site requirements was assessed. In addition, an evaluation of the adequacy and effectiveness of the DOE and the site contractors' management of ES ampersand H/quality assurance programs was conducted. This volume discusses findings concerning the environmental assessment
Full Text Available The Liberation Tigers of Tamil Eelam (LTTE was founded in 1976, demanding the establishment of an independent state for the Tamil ethnic minority in northern and northeastern Sri Lanka. In May 2009, following over three decades of conflict, its leader, Velupillai Prabhakaran was killed and the group was dismantled. The LTTE was established long before other well-known terror groups emerged, and yet it received little attention in comparison. An analysis of the relations between Sri Lankan governments and the Tamil Tigers from the onset of the struggle in the 1970s up to the group’s final defeat in May 2009 can provide valuable lessons to other democratic states fighting terrorist organizations, including Israel.
Leon-Saval, Sergio G; Bland-Hawthorn, Joss
We present a proof of concept compact diffraction limited high-resolution fiber-fed spectrograph by using a 2D multicore array input. This high resolution spectrograph is fed by a 2D pseudo-slit, the Photonic TIGER, a hexagonal array of near-diffraction limited single-mode cores. We study the feasibility of this new platform related to the core array separation and rotation with respect to the dispersion axis. A 7 core compact Photonic TIGER fiber-fed spectrograph with a resolving power of around R~31000 and 8 nm bandwidth in the IR centered on 1550 nm is demonstrated. We also describe possible architectures based on this concept for building small scale compact diffraction limited Integral Field Spectrographs (IFS).
This draft document contains findings identified during the Tiger Team Compliance Assessment of the US Department of Energy Savannah River Site (SRS), located in three counties (Aiken, Barnwell and Allendale), South Carolina. The Assessment was directed by the Department's Office of the Assistant Secretary for Environment, Safety, and Health (ES ampersand H) and was conducted from January 29 to March 23, 1990. The Savannah River Site Tiger Team Compliance Assessment was broad in scope covering the Environment, Safety and Health, and Management areas and was designed to determine the site's compliance with applicable Federal (including DOE), state, and local regulations and requirements. The scope of the Environmental assessment was sitewide while the Safety and Health assessments included site operating facilities (except reactors), and the sitewide elements of Aviation Safety, Emergency Preparedness, Medical Services, and Packaging and Transportation
The Office of Special Projects in the Office of the Assistant Secretary for Environment, Safety, and Health (EH) has the responsibility to conduct Tiger Team Assessments for the Secretary of Energy. This report documents the Tiger Team Assessment of the Oak Ridge K-25 Site (K-25 Site), Oak Ridge, Tennessee. The purpose of the Assessment is to provide the Secretary of Energy with concise information on the: current ES ampersand H compliance status of the Site and the vulnerabilities associated with that compliance status; root causes for noncompliance; adequacy of DOE and site contractor ES ampersand H management programs; adequacy of response actions developed to address identified problem areas; and adequacy of ES ampersand H self-assessments and the institutionalization of the self-assessment process at the K-25 Site
Menzies, Brandon R.; Renfree, Marilyn B.; Heider, Thomas; Mayer, Frieder; Hildebrandt, Thomas B.; Pask, Andrew J
The Tasmanian tiger or thylacine was the largest carnivorous marsupial when Europeans first reached Australia. Sadly, the last known thylacine died in captivity in 1936. A recent analysis of the genome of the closely related and extant Tasmanian devil demonstrated limited genetic diversity between individuals. While a similar lack of diversity has been reported for the thylacine, this analysis was based on just two individuals. Here we report the sequencing of an additional 12 museum-archived...
Carter, Neil H.; Riley, Shawn J.; Shortridge, Ashton; Shrestha, Binoj K.; Liu, Jianguo
In many regions around the world, wildlife impacts on people (e.g., crop raiding, attacks on people) engender negative attitudes toward wildlife. Negative attitudes predict behaviors that undermine wildlife management and conservation efforts (e.g., by exacerbating retaliatory killing of wildlife). Our study (1) evaluated attitudes of local people toward the globally endangered tiger (Panthera tigris) in Nepal’s Chitwan National Park; and (2) modeled and mapped spatial clusters of attitudes t...
The Liberation Tigers of Tamil Eelam (LTTE) was founded in 1976, demanding the establishment of an independent state for the Tamil ethnic minority in northern and northeastern Sri Lanka. In May 2009, following over three decades of conflict, its leader, Velupillai Prabhakaran was killed and the group was dismantled. The LTTE was established long before other well-known terror groups emerged, and yet it received little attention in comparison. An analysis of the relations between Sri Lankan go...
This document contains responses and planned actions that address the findings of the Tiger Team Assessment of Brookhaven National Laboratory, June 1990. In addition, the document contains descriptions of the management and organizational structure to be used in conducting planned actions, root causes for the problems identified in the findings, responses, planned actions, schedules and milestones for completing planned actions, and, where known, costs associated with planned actions.
Schmidtke, Gerhard; Radicella, Sandro M.; Jacobi, Christoph; Thuillier, Gerard; Nikutowski, Bernd; Erhardt, Christian; Eparvier, Francis G.
The 10th TIGER/COSPAR symposium raises the questions: What have been the issues, tasks and aims of TIGER at the end of the 20th century? Where we will have to go now? - Over the span of the TIGER initiative great strides have been made in observing and understanding the EUV solar spectral irradiance. Carefully calibrated observations from TIMED-SEE, SDO-EVE, SOLACES and SOLSPEC, PROBA-2 LYRA, and others have provided new insights into this important and highly variable energy input to the geospace environment. Agreement in terms of variability and absolute scales between recent solar irradiance data sets will be shown as well as improvements in solar and atmosphere irradiance modeling. Precise and continuous EUV measurements can be used to monitor solar variability and its effect on the upper atmosphere/ionosphere (T/I) system at time scales from days to decades by using them as input for ionospheric models or ionospheric proxies or indices. Even more, there is growing interest in scientific support for further improving the GNSS data evaluation which for new on-line EUV/UV measuring methods with airglow monitoring will be discussed. These methods would also improve the quantitative monitoring of space weather effects in the geospheric T/I system.
Tiger Team Assessments were implemented in June 1989 as part of a strategy to ensure that DOE facilities fully comply with Federal, state, local and DOE environment, safety, and health (ES ampersand H) requirements. The Tiger Teams provide the Secretary of Energy with information on current ES ampersand H compliance status of each DOE facility and causes for noncompliance. To date, Tiger Team Assessments have been completed at 25 DOE facilities. With regard to assessments of environmental restoration activities, the performance of DOE facilities was evaluated against the requirements of the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA), as amended, the National Contingency Plan (NCP), and DOE Order 5400.4, CERCLA Requirements, among others. Five major categories of environmental restoration-related findings were identified: (1) environmental restoration program planning and management (found at 60 percent of the sites assessed); (2) community relations/administrative record (60 percent); (3) characterization of extent of contamination (56 percent); (4) identification and evaluation of inactive waste sites (56 percent); and (5) DOE and NCP requirements for response action studies (44 percent). Primary causal factors for these findings were inadequate procedures, resources, supervision, and policy implementation
This report documents the results of the Department of Energy's (DOE's) Tiger Team Assessment of the Lawrence Berkeley Laboratory (LBL) conducted from January 14 through February 15, 1991. The purpose of the assessment was to provide the Secretary of Energy with the status of environment, safety, and health (ES ampersand H) programs at LBL. The Tiger Team concluded that curtailment of cessation of any operations at LBL is not warranted. However, the number and breadth of findings and concerns from this assessment reflect a serious condition at this site. In spite of its late start, LBL has recently made progress in increasing ES ampersand H awareness at all staff levels and in identifying ES ampersand H deficiencies. Corrective action plans are inadequate, however, many compensatory actions are underway. Also, LBL does not have the technical expertise or training programs nor the tracking and followup to effectively direct and control sitewide guidance and oversight by DOE of ES ampersand H activities at LBL. As a result of these deficiencies, the Tiger Team has reservations about LBL's ability to implement effective actions in a timely manner and, thereby, achieve excellence in their ES ampersand H program. 4 figs., 24 tabs
This report documents the Tiger Team Assessment of the buildings, facilities, and activities at the Stanford Linear Accelerator Center (SLAC) and the Stanford Synchrotron Radiation Laboratory (SSRL) near San Francisco, California. SLAC/SSRL is the twenty-eighth DOE site to be assessed by a Tiger Team. SLAC and SSRL are single-purpose laboratories. SLAC is dedicated to experimental and theoretical research in elementary particle physics and to the development of new techniques in high-energy accelerators and elementary particle detectors. SSRL is dedicated to research in atomic and solid-state physics, chemistry, biology, and medicine. The purpose of the SLAC/SSRL Tiger Team Assessment is to provide the Secretary of Energy with concise information on the following: current ES ampersand H compliance status at the site and the vulnerabilities associated with that compliance status; root causes for noncompliance; adequacy of DOE and SLAC/SSRL ES ampersand H management programs; response actions to address identified problem areas; and effectiveness of self-assessment
This report documents the results of the Department of Energy's (DOE's) Tiger Team Assessment of the Lawrence Berkeley Laboratory (LBL) conducted from January 14 through February 15, 1991. The purpose of the assessment was to provide the Secretary of Energy with the status of environment, safety, and health (ES H) programs at LBL. The Tiger Team concluded that curtailment of cessation of any operations at LBL is not warranted. However, the number and breadth of findings and concerns from this assessment reflect a serious condition at this site. In spite of its late start, LBL has recently made progress in increasing ES H awareness at all staff levels and in identifying ES H deficiencies. Corrective action plans are inadequate, however, many compensatory actions are underway. Also, LBL does not have the technical expertise or training programs nor the tracking and followup to effectively direct and control sitewide guidance and oversight by DOE of ES H activities at LBL. As a result of these deficiencies, the Tiger Team has reservations about LBL's ability to implement effective actions in a timely manner and, thereby, achieve excellence in their ES H program. 4 figs., 24 tabs.
Sadler, Ryan A; Ramsay, Edward; McAloose, Denise; Rush, Robert; Wilkes, Rebecca P
Canine distemper virus (CDV) has caused clinical disease and death in nondomestic felids in both captive settings and in the wild. Outbreaks resulting in high mortality rates in tigers (Panthera tigris) have prompted some zoos to vaccinate tigers for CDV. In this study, six tigers received a recombinant canarypox-vectored CDV vaccine (1 ml s.c.) and were revaccinated with 3 ml s.c. (mean) 39 days later. Blood collection for CDV antibody detection via serum neutralization was performed on (mean) days 0, 26, and 66 post-initial vaccination. No tigers had detectable antibodies at days 0 or 26, and only two tigers had low (16 and 32) antibody titers at day 66. Eight additional tigers received a live, attenuated CDV vaccine (1 ml s.c.) on day 0 and were revaccinated with 1 ml s.c. (mean) 171 days later. Blood collection for CDV antibody detection via serum neutralization was performed on (mean) days 0, 26, 171, and 196. Seven of eight tigers receiving the live, attenuated vaccine had no detectable titers prior to vaccination, but all animals had titers of >128 (range 128-1,024) at day 26. At 171 days, all tigers still had detectable titers (geometric mean 69.8, range 16-256), and at 196 days (2 wk post-revaccination) all but two showed an increase to >128 (range 32-512). To determine safety, an additional 41 tigers were vaccinated with 2 ml of a recombinant vaccine containing only CDV components, and an additional 38 tigers received 1 ml of the live, attenuated vaccine, administered either subcutaneously or intramuscularly; no serious adverse effects were noted. Although both vaccines appear safe, the live, attenuated vaccine produced a stronger and more consistent serologic response in tigers. PMID:27468029
Full Text Available The ranging behaviour of translocated problem tigers is poorly understood. The demand for releasing problem tigers back to the wild increases following the increasing the number of problem tigers that needs to be rescued in Sumatra in the last decade. In this study we estimate the home range size and obtain information on daily range of four translocated problem tigers, as well as discussing some potential factors determining the size of home range and their movement. We translocated four adult males Sumatran tigers (Panthera tigris sumatrae caught after killing domestic animals or rescued from traps set up by villagers for capturing deer and wild boar. The tigers were released following 16-225 days rehabilitation. All were fitted with global positioning system collars and released 74-1,350 km from their capture site. The length of time needed by each tiger for establishing home range was between 6 and 13 weeks. The home range size of each individual tiger estimated with 100% minimum convex polygon varies between 67.1 km2 and 400 km2, while estimations with a 95% fixed kernel methods were between 37.5 km2 and 188.1 km2. The difference in home range size established by each translocated tigers indicates the variability of the range size even within a subspecies. The maximum distance moved each tiger in one day was different, the range was 8.5-18.9 km. Although preliminary, these data may be useful for improving future translocation of problem tiger, as this study was the first ever conducted in Sumatra.
..., Louisiana; --Questar Exploration and Production Company (Questar) M/R Station in Red River Parish, Louisiana... Energy Regulatory Commission ETC Tiger Pipeline Company, LLC; Notice of Availability of the Environmental... Pipeline Project (Project) proposed by ETC Tiger Pipeline Company, LLC (ETC Tiger) in the above...
... Energy Regulatory Commission ETC Tiger Pipeline, LLC; Notice of Intent To Prepare an Environmental Assessment for the Proposed ETC Tiger Pipeline Expansion Project--Phase I and Request for Comments on... Tiger Pipeline Expansion Project--Phase I, involving construction and operation of facilities by...
... rule to list the Salt Creek tiger beetle as endangered was published on October 6, 2005 (70 FR 58335..., 2005 (70 FR 58335). Taxonomy and Species Description The Salt Creek tiger beetle (Cicindela nevadica... size. The Salt Creek tiger beetle is historically known from six populations (70 FR 58336, October...