Sample records for bk010044 beckman coulter

  1. CLSI-based transference of CALIPER pediatric reference intervals to Beckman Coulter AU biochemical assays. (United States)

    Abou El Hassan, Mohamed; Stoianov, Alexandra; Araújo, Petra A T; Sadeghieh, Tara; Chan, Man Khun; Chen, Yunqi; Randell, Edward; Nieuwesteeg, Michelle; Adeli, Khosrow


    The CALIPER program has established a comprehensive database of pediatric reference intervals using largely the Abbott ARCHITECT biochemical assays. To expand clinical application of CALIPER reference standards, the present study is aimed at transferring CALIPER reference intervals from the Abbott ARCHITECT to Beckman Coulter AU assays. Transference of CALIPER reference intervals was performed based on the CLSI guidelines C28-A3 and EP9-A2. The new reference intervals were directly verified using up to 100 reference samples from the healthy CALIPER cohort. We found a strong correlation between Abbott ARCHITECT and Beckman Coulter AU biochemical assays, allowing the transference of the vast majority (94%; 30 out of 32 assays) of CALIPER reference intervals previously established using Abbott assays. Transferred reference intervals were, in general, similar to previously published CALIPER reference intervals, with some exceptions. Most of the transferred reference intervals were sex-specific and were verified using healthy reference samples from the CALIPER biobank based on CLSI criteria. It is important to note that the comparisons performed between the Abbott and Beckman Coulter assays make no assumptions as to assay accuracy or which system is more correct/accurate. The majority of CALIPER reference intervals were transferrable to Beckman Coulter AU assays, allowing the establishment of a new database of pediatric reference intervals. This further expands the utility of the CALIPER database to clinical laboratories using the AU assays; however, each laboratory should validate these intervals for their analytical platform and local population as recommended by the CLSI. Copyright © 2015 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  2. CLSI-based transference of the CALIPER database of pediatric reference intervals to Beckman Coulter DxC biochemical assays. (United States)

    Araújo, Petra A T; Thomas, Dylan; Sadeghieh, Tara; Bevilacqua, Victoria; Chan, Man Khun; Chen, Yunqi; Randell, Edward; Adeli, Khosrow


    The CALIPER program has established a comprehensive database of age- and sex-stratified pediatric reference intervals for over 85 common biochemical markers, largely using the Abbott ARCHITECT assays. To allow a broader application of the CALIPER database, we examined transference to 36 Beckman Coulter Synchron Unicel DxC800 assays, based on the CLSI C28-A3/EP9-A3 guidelines. Patient sample comparisons were performed for 36 biochemical assays using 200 serum specimens obtained from pediatric patients on the Abbott ARCHITECT ci8200 and the Beckman Coulter DxC800. For each analyte, R(2) values were calculated to assess the quality of correlation between the platforms. Statistical criteria used to assess transferability included a) regression analysis to create the equation of the line of best fit, b) standardized residual, c) Bland-Altman, and d) quantile-quantile plots. Transferred reference intervals were further verified by analyzing serum samples from 100 healthy children from the CALIPER cohort on the Beckman Coulter system. The reference intervals for most of the assessed analytes were transferable to Beckman Coulter assays (31 out of 36 studied) and the newly calculated reference intervals were verified through analysis of CALIPER reference samples (28 out of 31). Eighteen assays demonstrated excellent correlation (R(2)≥0.95), and 13 assays showed strong correlation (0.77≤R2≤0.94). The current study allowed successful transference of a large number of biochemical markers from the CALIPER database to assays on the Beckman Coulter DxC800 platform. Transference should facilitate broader application of CALIPER reference intervals at pediatric centers using DxC biochemical assays. Copyright © 2015 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  3. Multi-center evaluation of analytical performance of the Beckman Coulter AU5822 chemistry analyzer. (United States)

    Zimmerman, M K; Friesen, L R; Nice, A; Vollmer, P A; Dockery, E A; Rankin, J D; Zmuda, K; Wong, S H


    Our three academic institutions, Indiana University, Northwestern Memorial Hospital, and Wake Forest, were among the first in the United States to implement the Beckman Coulter AU5822 series chemistry analyzers. We undertook this post-hoc multi-center study by merging our data to determine performance characteristics and the impact of methodology changes on analyte measurement. We independently completed performance validation studies including precision, linearity/analytical measurement range, method comparison, and reference range verification. Complete data sets were available from at least one institution for 66 analytes with the following groups: 51 from all three institutions, and 15 from 1 or 2 institutions for a total sample size of 12,064. Precision was similar among institutions. Coefficients of variation (CV) were Analytes with CVs >10% included direct bilirubin and digoxin. All analytes exhibited linearity over the analytical measurement range. Method comparison data showed slopes between 0.900-1.100 for 87.9% of the analytes. Slopes for amylase, tobramycin and urine amylase were 1.5, due to known methodology or standardization differences. Consequently, reference ranges of amylase, urine amylase and lipase required only minor or no modification. The four AU5822 analyzers independently evaluated at three sites showed consistent precision, linearity, and correlation results. Since installations, the test results had been well received by clinicians from all three institutions. Copyright © 2015. Published by Elsevier Inc.

  4. [Evaluation of the analyzer of hematology Beckman Coulter® HmX™ in the university hospital of Oran]. (United States)

    Zmouli, N; Moulasserdoun, K; Seghier, F


    The choice of an automaton of haematology is a determining stage, which has to take into account at the same time the quality of the results and the economic imperatives: workload, structure and organization of the laboratory. [corrected] It is in this spirit that we estimated during a period of 3 months the analyzer of haematology: the HmX™ Coulter with boatman of samples of the company Beckman. This automaton realizes the blood numeration, the formula leukocytic and the reticulocyte count. At first, we estimated the appropriate characteristics of device. Secondly, we estimated the relevance, the sensibility and the specificity of the alarms by comparing with the reference method, which is the optical microscopy. For that purpose, 125 blood smears resulting from service of haematology and from resuscitation were examined in optical microscopy. The technical tests were realized according to the recommendations of the International committee for evaluation of automatons of haematology. The analytical performances were satisfactory in particular the big interval of linearity and the absence of contamination. As regards the evaluation of the alarms system: rate of rejection is 63%, the sensibility 86%, the specificity 70%, the positive predictive value 80%, the negative predictive value 78% and the efficiency 80%. The alarms myelaemia and atypical lymphocytes were never sources of false negatives. The alarms erythroblasts and platelet aggregates did not engendered positive forgery. The blast cell alarm was responsible for a single case of false negative. The faithfulness of automaton is satisfactory: the absence of contamination, the big interval of linearity for the leukocytes, the red blood cells and the platelets as well as a good relevance of the alarms with regard to the anomalies found on the peripheral blood smear. From the user-friendliness and practicability point of view, the HmX™ Coulter was deeply appreciated. Copyright © 2013 Elsevier Masson SAS. All

  5. 'Aussie Normals': an a priori study to develop reference intervals in a healthy Australian population using the Beckman Coulter LH 750 Haematology Analyser as candidates for harmonised values. (United States)

    Koerbin, G; Potter, J M; Andriolo, K; West, N P; Glasgow, N; Hawkins, C; Cavanaugh, J A; Hickman, P E


    Reference limits or intervals are important benchmarks or tools that help the clinician to distinguish between a result that is most likely to lie within a 'healthy' or diseased category. It has been suggested that a review of haematology reference intervals is long overdue. In this study we report on our findings for analytes routinely measured in a complete blood count (CBC) performed on the Beckman Coulter LH 750 analyser and an additional comparative study using the Beckman Coulter LH 750, the Sysmex XN and Abbott Sapphire. The results from the comparative study indicate that bias would not prevent harmonisation of reference intervals for these common haematology parameters. The results offered by the Aussie Normals study represent good candidates as the basis for harmonisation reference intervals. Copyright © 2017 Royal College of Pathologists of Australasia. All rights reserved.

  6. A Comparison of Mindray BC-6800, Sysmex XN-2000, and Beckman Coulter LH750 Automated Hematology Analyzers: A Pediatric Study. (United States)

    Ciepiela, Olga; Kotuła, Iwona; Kierat, Szymon; Sieczkowska, Sandra; Podsiadłowska, Anna; Jenczelewska, Anna; Księżarczyk, Karolina; Demkow, Urszula


    Modern automated laboratory hematology analyzers allow the measurement of over 30 different hematological parameters useful in the diagnostic and clinical interpretation of patient symptoms. They use different methods to measure the same parameters. Thus, a comparison of complete blood count made by Mindray BC-6800, Sysmex XN-2000 and Beckman Coulter LH750 was performed. A comparison of results obtained by automated analysis of 807 anticoagulated blood samples from children and 125 manual microscopic differentiations were performed. This comparative study included white blood cell count, red blood cell count, and erythrocyte indices, as well as platelet count. The present study showed a poor level of agreement between white blood cell enumeration and differentiation of the three automated hematology analyzers under comparison. A very good agreement was found when comparing manual blood smear and automated granulocytes, monocytes, and lymphocytes differentiation. Red blood cell evaluation showed better agreement than white blood cells between the studied analyzers. To conclude, studied instruments did not ensure satisfactory interchangeability and did not facilitate a substitution of one analyzer by another. © 2016 Wiley Periodicals, Inc.

  7. Clinical utility of Beckman-Coulter Gen's reticulocyte analysis in the study of anemia of chronic disease (ACD). (United States)

    Rodrigues, Ana; Ortega, Carlos; Santos, Luís; Teixeira, Alexandre; Dinis, Maria Joáo; Vasconcelos, Iponina; Lacerda, Jorge; Fonseca, Elisa


    The aim of this study is to analyze the reticulocyte parameters (classical and research parameters) acquired by the Beckman Coulter GEN'S blood counter (GEN'S; Brea, CA, USA) to establish the "reticulocyte profile" characteristics of patients with anemia of chronic disease (ACD). The reticulocyte parameters and profile provided by the GEN'S were studied in 38 anemic patients who fulfilled the diagnostic criteria for the ACD, and the results were compared with those of 38 healthy controls in a multivariate statistical analysis using the Student t-test and the receiver operating characteristics (ROC) curve analysis.Statistically significant (P 0.9), for the IRF (high) and the reticulocyte population data: mean channel scatter retics (high) and the mean channel conductivity retics (high). This study establishes the reticulocyte parameters and reticulocyte profile as provided by the GEN'S and characteristic of patients with ACD as compared with normal subjects. This should provide the basis for further studies comparing the reticulocyte profile of patients with ACD with those found in other types of anemia.

  8. The effect of sodium bicarbonate and validation of beckman coulter AU680 analyzers for measuring total carbon dioxide (TCO2) concentrations in horse serum. (United States)

    Dirikolu, Levent; Waller, Pamela; Waguespack, Mona Landry; Andrews, Frank Michael; Keowen, Michael Layne; Gaunt, Stephen David


    This study evaluated the usage of Beckman Coulter AU680 analyzers for measurement of TCO 2 in horse serum, and the effect of sodium bicarbonate administrations on serum TCO 2 levels in resting horses. Treatment of horses with sodium bicarbonate did not result in any adverse events. Mean TCO 2 concentration was significantly higher from 1 to 8 h in the sodium bicarbonate-treated horses compared to the untreated controls. Within an hour, administration of sodium bicarbonate increased the TCO 2 level from 31.5 ± -2.5 (SD) to 34.0 ± 2.65 (SD) mmol/L and at 2-8 h post-administration, the TCO 2 level was above the 36 mmol/L cut-off level. In all quality control analysis of Australian standard by Beckman Coulter AU680 analyzer, the instrument slightly over estimated the TCO 2 level but the values were in close agreement with mean TCO 2 level being 38.03 with ± 0.87 mmol/L (SD). Expanded uncertainty was calculated using different levels of confidence interval. Based on 99.5% confidence interval using 0.805% expanded uncertainty using mean measured concentration of 38.05 mmol/L, it was estimated that any race samples TCO 2 level higher than 38.5 mmol/L will be indicative of sodium bicarbonate administration using Beckman Coulter AU680 analyzer in Louisiana.

  9. Pediatric reference value distributions and covariate-stratified reference intervals for 29 endocrine and special chemistry biomarkers on the Beckman Coulter Immunoassay Systems: a CALIPER study of healthy community children. (United States)

    Karbasy, Kimiya; Lin, Danny C C; Stoianov, Alexandra; Chan, Man Khun; Bevilacqua, Victoria; Chen, Yunqi; Adeli, Khosrow


    The CALIPER program is a national research initiative aimed at closing the gaps in pediatric reference intervals. CALIPER previously reported reference intervals for endocrine and special chemistry markers on Abbott immunoassays. We now report new pediatric reference intervals for immunoassays on the Beckman Coulter Immunoassay Systems and assess platform-specific differences in reference values. A total of 711 healthy children and adolescents from birth to CALIPER Pediatric Reference Interval database will enable accurate diagnosis and laboratory assessment of children monitored by Beckman Coulter Immunoassay Systems in health care institutions worldwide. These reference intervals must however be validated by individual labs for the local pediatric population as recommended by CLSI.

  10. Analytical interference by monoclonal immunoglobulins on the direct bilirubin AU Beckman Coulter assay: the benefit of unsuspected diagnosis from spurious results. (United States)

    García-González, Elena; González-Tarancón, Ricardo; Aramendía, Maite; Rello, Luis


    Monoclonal (M) components can interfere with the direct bilirubin (D-Bil) assay on the AU Beckman Coulter instrumentation and produce spurious results, such as D-Bil values greater than total bilirubin (T-Bil) or very low/negative D-Bil values. If properly detected, this interference may uncover undiagnosed patients with monoclonal gammopathy (MG). We investigated the interference rate on the D-Bil AU assay in serum samples known to contain M proteins along with their isotype and described the protocol set up in our laboratory to help with the diagnosis of MG based on D-Bil spurious results as first indication. During a period of 4 years, 15.4% (345 of 2235) of serum samples containing M immunoglobulins produced erroneous D-Bil results, although no clear relationship between the magnitude or isotype of the M component and interference could be found. In total 22 new patients were diagnosed with MG based on the analytical artefact with the D-Bil as first indication. The D-Bil interference from MG on the Beckman AU analysers needs to be made known to laboratories in order to prevent clinical confusion and/or additional workup to explain the origin of anomalous results. Although this information may not add to the management of existing patients with serum paraproteins, it can benefit patients that have not been diagnosed with MG by triggering follow up testing to determine if M components are present.

  11. Comparison of Impedance Platelet Count by Sysmex XE-5000 and Beckman Coulter LH 780 with Optical Fluorescent Platelet Count in Thalassemia Patients

    Directory of Open Access Journals (Sweden)

    Chaicharoen Tantanate, M.D.


    Full Text Available Background: Red blood cell (RBC abnormalities are frequently found among thalassemia patients and have been reported to be a cause of spuriously high platelet counts. Objective: To compare impedance platelet count by Sysmex XE-5000 (PLT-XE and Beckman Coulter LH 780 (PLT-LH with optical fluorescent platelet count (PLT-O in thalassemia patients. Methods: EDTA blood specimens from 152 thalassemia patients were analyzed by routine mode of PLT-XE and PLT-LH. The PLT-O run on reticulocyte mode of Sysmex XE-5000 was used as a reference method. Spearman’s rank correlation coefficient (rs and Bland-Altman plot were analyzed. Results: Correlations between impedance and PLT-O methods were excellent, as demonstrated by rs of 0.95 and 0.97 for PLT-XE and PLT-LH, respectively. However, there was no agreement between impedance and PLT-O methods. There were differences in biases of PLT-XE between thalassemia minor and both thalassemia intermedia and major. Compared to PLT-O, the concordance rates of diagnosis for platelet count abnormality were 94.7% and 96.7% for PLT-XE and PLT-LH, respectively. Conclusion: Compared to PLT-O, impedance method was associated with spuriously high platelet counts in thalassemia patients. Careful blood smear examination and/or accurate assay should be used in cases suspected of spuriously high platelet count.

  12. Practical application of biological variation and Sigma metrics quality models to evaluate 20 chemistry analytes on the Beckman Coulter AU680. (United States)

    Tran, Mai Thi Chi; Hoang, KienTrung; Greaves, Ronda F


    This study aimed to evaluate the imprecision and bias data generated for 20 routine chemistry analytes against both the biological variation fitness for purpose (FFP) and Sigma metrics (SM) criteria. Twenty serum/plasma analytes were evaluated on the Beckman Coulter AU680. Third party commercial lyophilized internal quality control samples of human origin were used for day-to-day imprecision calculations. Commercial external quality assurance (EQA) samples were used to determine the systematic error between the test method result and the instrument group mean result from the EQA program for each analyte. Biological variation data was used to calculate the minimum, desirable and optimal imprecision and bias for determination of FFP. The desirable total allowable error was determined from biological variation data and applied to the SM calculation. The outcomes of both quality approaches were then compared. The day-to-day imprecision of most tested analytes (except sodium and chloride) were smaller than the allowable imprecision (ranging from minimum to optimum). Most analytes achieved at least minimum bias. The SM varied with analyte concentration with six analytes producing low Sigma values. Comparing the quality processes eleven analytes produced a green light for both FFP and SM. There was some difference seen in interpretation for the other nine analytes. The individual interpretation of bias and imprecision using FFP criteria allowed for the clear determination of the major source of error. Whereas, SM provided a summative evaluation of method performance. But the selection of total allowable error (TEa) is fundamental to this interpretation and harmonisation of the TEa calculation is needed. Copyright © 2016 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  13. Microchip Coulter particle counter

    DEFF Research Database (Denmark)

    Larsen, Ulrik Darling; Blankenstein, Gert; Branebjerg, J.


    This paper presents a micro device employing the Coulter principle for counting and sizing of living cells and particles in liquid suspension. The microchip Coulter particle counter (μCPC) has been employed in a planar silicon structure covered with glass, which enables detailed observation during...... and short contact time of liquids in microchannels. As a result, the width of the liquids can be controlled without knowing the actual flow rates. The μCPC has been fabricated by standard microfabrication techniques, including RIE, wet silicon etching, metalization and anodic bonding...

  14. Wallace Coulter's life and his impact on the world. (United States)

    Simson, E


    2013 is the centenary of Wallace Coulter's birth. He was an engineer, inventor, entrepreneur and visionary. He transformed the practice of laboratory hematology with his invention of the Coulter Principle and its application to blood cell analysis, together with the company he founded to bring it and his subsequent inventions and innovations to the world. He was born in modest circumstances and he remained modest in his outlook on life, despite his magnificent achievements, his successes, his numerous prestigious awards and his wealth later in life. This article traces his early life, his career, his achievements and the immense benefits he brought to the people of this planet. © 2013 Blackwell Publishing Ltd.

  15. Sensor and control for consistent seed drill coulter depth

    DEFF Research Database (Denmark)

    Kirkegaard Nielsen, Søren; Nørremark, Michael; Green, Ole

    The even placement of seeds at the correct depth is crucial for achieving the optimum yield. The depth of drill coulters on state-of-the-art seeding machines is normally set manually by downforce springs or weights will therefore react to different soil resistances. A prototype seeder with one...

  16. Sensor and control for consistent seed drill coulter depth

    DEFF Research Database (Denmark)

    Kirkegaard Nielsen, Søren; Nørremark, Michael; Green, Ole


    The consistent depth placement of seeds is vital for achieving the optimum yield of agricultural crops. In state-of-the-art seeding machines, the depth of drill coulters will vary with changes in soil resistance. This paper presents the retrofitting of an angle sensor to the pivoting point...

  17. Seed drill instrumentation for spatial coulter depth measurements

    DEFF Research Database (Denmark)

    Kirkegaard Nielsen, Søren; Munkholm, Lars Juhl; Lamandé, Mathieu


    An even and correct depth placement of seeds is crucial for uniform crop germination and for obtaining the desired agricultural yield. On state-of-the-art seed drills, the coulter down pressure is set manually by static springs or heavy weights, which entails that the coulter’s seeding depth reacts...

  18. [Evaluation of enzyme modules of the Beckman Astra 8]. (United States)

    Glikmanas, G; Lormeau, N; Gourmelin, Y; Hersant, J; Truchaud, A


    We evaluated the analytical performances of the Beckman Astra 8 enzyme analyser which allows the determination of the 6 enzymes (creatine kinase, Aspartate aminotransferase, Alanine aminotransferase, gamma glutamyl-transferase, lactate dehydrogenase, Alcaline Phosphatases), in a same serum sample with only two modules. We compared the results obtained in the healthy and pathological subject's sera with those given by a centrifugal analyser IL Multistat III (Delhomme) using reagents and temperature recommended by SFBC (Société Française de Biologie Clinique). Instrument utilization is greatly helped by various alarms and diagnostic programmes. Results for emergency and routine determinations accord with those announced by the manufacturer and those required for a modern enzymatic analyzer.

  19. An extinction scale-expansion unit for the Beckman DK2 spectrophotometer (United States)

    Dixon, M.


    The paper describes a simple but accurate unit for the Beckman DK2 recording spectrophotometer, whereby any 0·1 section of the extinction (`absorbance') scale may be expanded tenfold, while preserving complete linearity in extinction. PMID:6048800

  20. Label-free protein detection using a microfluidic Coulter-counter device

    DEFF Research Database (Denmark)

    Rodriguez-Trujíllo, Romén; Ajine, Mohammad Akram; Orzan, A.


    A new method for measuring specific protein concentrations in solutions has been developed. The technique makes use of the Coulter effect for detecting and sizing of micro-scaled objects suspended in a buffer fluid. The method is completely label-free as it is only based on the electrical readout...

  1. Data transfer from Beckman LS 5800 liquid scintillation counters to IBM personal computers. (United States)

    Maan, A C


    This communication describes a short routine in BASICA for the IBM-PC, written to collect data from a Beckman liquid scintillation counter. In the form presented here the routine converts incoming bytes into separate lines and saves these lines in a file. There are many possible applications for further use of the data in these files. A few suggestions are given as to the format in which data can be stored and how to process these data automatically after all samples have been counted. The only hardware needed is an asynchronous communications adapter for the IBM-PC and an RS232 cable.

  2. Automation of a Beckman liquid scintillation counter for data capture and data-base management

    International Nuclear Information System (INIS)

    Neil, W.; Irwin, T.J.; Yang, J.J.


    A software package for the automation of a Beckman LS9000 liquid scintillation counter is presented. The package provides effective on-line data capture (with a Perkin Elmer 3230 32-bit minicomputer), data-base management, audit trail and archiving facilities. Key features of the package are rapid and flexible data entry, background subtraction, half-life correction, ability to queue several sample sets pending scintillation counting, and formatted report generation. A brief discussion is given on the development of customized data processing programs. (author)

  3. The Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin.

    LENUS (Irish Health Repository)

    Byrne, Brendan


    Commercially available prolactin immunoassays detect macroprolactin to variable degrees. Best practice requires laboratories to assess the cross-reactivity of their prolactin assay with macroprolactin, and where appropriate, introduce a screen for the presence of macroprolactin. Our policy has been to reanalyse hyperprolactinaemic samples following polyethylene glycol (PEG) precipitation and to report the resultant value as the monomeric prolactin content of the sample. The goal of this study was to determine the need to continue PEG precipitation when prolactin measurements with the Wallac AutoDELFIA were replaced by the Beckman DxI 800.

  4. Disturbance of Ultisol soil based on interactions between furrow openers and coulters for the no-tillage system

    Energy Technology Data Exchange (ETDEWEB)

    Francetto, T.R.; Alonço, A. dos S.; Brandelero, C.; Machado, O.D. da C.; Veit, A.A.; Carpes, D.P.


    The present study evaluated the effect of different associations between coulters and fertilizer furrow openers on soil disturbance, furrow depth and width, according to forward speed. The study was conducted on a farm in Santa Maria (Brazil/RS), in soil classified as sandy loam Ultisol. The experiment consisted of 24 combinations of treatments with three replications in a 2×3×4 factorial experiment. The combinations were formed by the interaction of the factors including: two types of furrow openers (hoe and double-disc), three types of coulters (no-coulter, smooth and offset fluted) and four levels of forward speed (1.11, 1.67, 2.22 and 2.78 m/s). Soil elevation and soil disturbance area profiles were obtained with the use of a micro profilometer, and disturbance values were calculated with the aid of computer software program Auto Cad. The disturbance area was not affected by speed; it was greater when using the hoe opener, and in association with the offset fluted coulter. Speed was inversely proportional to the depth of the furrows made by the hoe opener. Furthermore, the hoe caused the greatest furrow width (0.26 m) in comparison with the double-disc (0.24 m). The use of different coulters associated with furrow openers increased this variable (0.23 m for the no-coulter condition, 0.25 m with smooth and 0.26 m with offset fluted). The use of coulters combined with furrow openers reduces soil swelling, in approximately 8% for the smooth and 20% for the offset fluted. (Author)

  5. CLSI-based transference of the CALIPER database of pediatric reference intervals from Abbott to Beckman, Ortho, Roche and Siemens Clinical Chemistry Assays: direct validation using reference samples from the CALIPER cohort. (United States)

    Estey, Mathew P; Cohen, Ashley H; Colantonio, David A; Chan, Man Khun; Marvasti, Tina Binesh; Randell, Edward; Delvin, Edgard; Cousineau, Jocelyne; Grey, Vijaylaxmi; Greenway, Donald; Meng, Qing H; Jung, Benjamin; Bhuiyan, Jalaluddin; Seccombe, David; Adeli, Khosrow


    The CALIPER program recently established a comprehensive database of age- and sex-stratified pediatric reference intervals for 40 biochemical markers. However, this database was only directly applicable for Abbott ARCHITECT assays. We therefore sought to expand the scope of this database to biochemical assays from other major manufacturers, allowing for a much wider application of the CALIPER database. Based on CLSI C28-A3 and EP9-A2 guidelines, CALIPER reference intervals were transferred (using specific statistical criteria) to assays performed on four other commonly used clinical chemistry platforms including Beckman Coulter DxC800, Ortho Vitros 5600, Roche Cobas 6000, and Siemens Vista 1500. The resulting reference intervals were subjected to a thorough validation using 100 reference specimens (healthy community children and adolescents) from the CALIPER bio-bank, and all testing centers participated in an external quality assessment (EQA) evaluation. In general, the transferred pediatric reference intervals were similar to those established in our previous study. However, assay-specific differences in reference limits were observed for many analytes, and in some instances were considerable. The results of the EQA evaluation generally mimicked the similarities and differences in reference limits among the five manufacturers' assays. In addition, the majority of transferred reference intervals were validated through the analysis of CALIPER reference samples. This study greatly extends the utility of the CALIPER reference interval database which is now directly applicable for assays performed on five major analytical platforms in clinical use, and should permit the worldwide application of CALIPER pediatric reference intervals. Copyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  6. Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry

    Directory of Open Access Journals (Sweden)

    Jennifer Pasquier


    Full Text Available The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp. The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading, we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.

  7. The John Stanley Coulter memorial lecture. Anatomy of change: the need for effective disability policy change agents. (United States)

    Silverstein, Robert


    The 2009 Coulter Lecture highlights the need for effective disability policy change agents to advocate for health care policy and research that focuses on optimizing the health and function of individuals with disabilities and chronic conditions-not only on their full restoration/cure. The lecture describes the "grotesque" historical treatment of persons with disabilities under the old policy framework, the treatment of people with disabilities under the new/emerging disability policy framework, and the critical role played by coalitions in bringing about progressive, sustainable change on behalf of individuals with disabilities. Copyright 2010 American Congress of Rehabilitation Medicine. Published by Elsevier Inc. All rights reserved.

  8. Effects of particle's off-axis position, shape, orientation and entry position on resistance changes of micro Coulter counting devices

    International Nuclear Information System (INIS)

    Qin, Zhenpeng; Zhe, Jiang; Wang, Guo-Xiang


    With the recent advance in micro/nano-fabrication technology, micro Coulter counters have been widely used in detecting and characterizing micro- and nanoscale objects. In this paper, the electrical resistance change during translocation of a non-conducting particle through a channel is studied numerically. The numerical results are validated by proven analytical results available in the literature. The effects of particle's off-axis position, shape and orientation, and entry position are studied for particles with a large dynamic range. From the numerical results, a new fitted correlation is proposed that can accurately predict the resistance change caused by off-axis spherical particles regardless of their size. The shape and orientation effects of the electrical resistance change are studied by changing the axis ratio of spheroid particles and their orientation angles. Results show that a particle's shape and orientation have a significant influence on the resistance change. Simulation of an entry effect indicates that a particle starts to induce a resistance change before it enters the channel and still causes a resistance change even after the particle exits the channel completely. This study will offer some guidelines in designing and implementing Coulter counting devices and experiments, and provide insights into explaining experimental results

  9. A new method for semi-automated quantitation of E-rosettes using a particle size analyser (Coulter Channelyzer). (United States)

    Brown, R A; Potts, R C; Robertson, A J; Hayes, P C; Ramesar, K; Beck, J S


    Visual counts of the number of SRBC attached to individual lymphocytes in E-rosette preparations from 3 healthy young adults were made by 3 experienced observers: from these data the size distribution profile of particles in each E-rosette preparation was calculated. In parallel experiments the size distribution profile was measured directly with a particle size analyser (Coulter Counter and Channelyzer): in every case the observed profile lay within the 95% confidence band around the reconstructed profiles. It was concluded that the Coulter Channelyzer profile is an accurate measurement of the E-rosette size distribution and that particle sizing could provide a reasonable basis for semi-automated tests for E-rosettes. A mathematical model has been developed by which the percentage of lymphocytes forming E-rosettes, the distribution of number of attached SRBC and the average avidity of the lymphocyte receptor for SRBC could be deduced from the size-distribution profiles. The method has a high degree of precision, and a good correlation (P less than 0.01) was obtained when the percentage of E-rosettes (defined for this purpose as having three or more attached SRBC) obtained from the new method was compared with results from standard light microscopy.

  10. Successfully accelerating translational research at an academic medical center: the University of Michigan-Coulter translational research partnership program. (United States)

    Pienta, Kenneth J


    Translational research encompasses the effective movement of new knowledge and discoveries into new approaches for prevention, diagnosis, and treatment of disease. There are many roadblocks to successful bench to bedside research, but few have received as much recent attention as the "valley of death". The valley of death refers to the lack of funding and support for research that moves basic science discoveries into diagnostics, devices, and treatments in humans, and is ascribed to be the result of companies unwilling to fund research development that may not result in a drug or device that will be utilized in the clinic and conversely, the fact that researchers have no access to the funding needed to carry out preclinical and early clinical development to demonstrate potential efficacy in humans. The valley of death also exists because bridging the translational gap is dependent on successfully managing an additional four risks: scientific, intellectual property, market, and regulatory. The University of Michigan (UM) has partnered with the Wallace H. Coulter Foundation (CF) to create a model providing an infrastructure to overcome these risks. This model is easily adoptable to other academic medical centers (AMCs). © 2010 Wiley Periodicals, Inc.

  11. Gastroprotective effect of diligustilide isolated from roots of Ligusticum porteri coulter & rose (Apiaceae) on ethanol-induced lesions in rats. (United States)

    Velázquez-Moyado, Josué A; Martínez-González, Alejandro; Linares, Edelmira; Bye, Robert; Mata, Rachel; Navarrete, Andrés


    The rhizome of Ligusticum porteri Coulter& Rose (LP) has been traditionally used by the ethnic group Raramuri in the North of México for treatment of diabetes, tuberculosis, stomachaches, diarrhea and ritual healing ceremonies. It is use as antiulcer remedy has been extended to all Mexico. To evaluate the gastroprotective activity of LP organic extracts and the major natural product diligustilide (DLG),using as experimental model the inhibition of the ethanol-induced lesions in rats. Gastric ulcers were induced by intragastric instillation of absolute ethanol (1 mL). We tested the gastroprotective activity of the organic extracts of LP and the pure compound DLG. The ulcer index (UI) was determined to measure the activity. In order to elucidate the action mechanism of DLG the animals were treated with L-NAME, N-ethylmalemide, Forskolin, 2',5'-dideoxyadenosine, Indomethacin, Glibenclameide, Diazoxide, NaHS and DL-Propargylglycine. The pylorus-ligated rat model was used to measure gastric secretion. The oral administration of organic extracts of Ligusticum porteri showed gastroprotective effect at 30 mg/Kg on ethanol induced gastric lesions; hexane and dichloromethane extracts were the most active. DLG was the major compound in the hexane extract. This compound at 10 mg/kg prevented significantly the gastric injuries induced by ethanol. The alkylation of endogenous non-protein-SH groups with N-ethylmaleimide abolished the gastroprotective effect of DLG and blocking the formation of endogenous prostaglandins by the pretreatment with indomethacin attenuated the gastroprotective effect of DLG. The gastroprotective activity demonstrated in this study tends to support the ethnomedical use of Ligusticum porteri roots. DLG, isolated as major compound of this medicinal plant has a clear gastroprotective effect on the ethanol-induced gastric lesions. The results suggest that the antiulcer activity of DLG depends on the participation of the endogenous non-protein -SH groups

  12. CRADA with Beckman Instruments and Pacific Northwest National Laboratory (PNL-013): Development and commercialization of the Unsaturated Flow Apparatus (UFA) using characterization of aridisols

    Energy Technology Data Exchange (ETDEWEB)

    Wright, J.; Conca, J.


    The objective of this Cooperative Research and Development Agreement (CRADA) was to develop and commercialize a technology conceived by scientists at Pacific Northwest National Laboratory (PNNL) and manufactured by Beckman Instruments, Inc. (Beckman), and to apply this technology to the characterization of and soils. The technology is the Unsaturated Flow Apparatus (UFA). The UFA provides a highly efficient method of direct, rapid measurement of hydraulic conductivity and other flow properties according to Darcy-Buckingham principles because the operator controls both the fluid driving force, using an ultracentrifuge, and the flow into the sample while it is spinning, with a rotating seal assembly. The concept of using centrifugation to significantly decrease the time needed, from years or months to days, for study of subsurface transport, particularly under unsaturated conditions, was conceived by James Conca, Ph.D., and Judith Wright, Ph.D., in 1986. The prototype UFA was developed in 1988 because there was a need to rapidly and accurately determine transport parameters in soils, sediments, and rocks for the Grout Waste Disposal Program. Transport parameters are critical to modeling outcomes for site-specific solutions to environmental remediation and waste disposal problems.

  13. Establishment of reference intervals for serum thyroid-stimulating hormone, free and total thyroxine, and free and total triiodothyronine for the Beckman Coulter DxI-800 analyzers by indirect method using data obtained from Chinese population in Zhejiang Province, China. (United States)

    Wang, Yan; Zhang, Yu-Xia; Zhou, Yong-Lie; Xia, Jun


    In order to establish suitable reference intervals of thyroid-stimulating hormone (TSH), free (unbound) T4 (FT4), free triiodothyronine (FT3), total thyroxine (T4), and total triiodothyronine (T3) for the patients collected in Zhejiang, China, an indirect method was developed using the data from the people presented for routine health check-up. Fifteen thousand nine hundred and fifty-six person's results were reviewed. Box-Cox or Case Rank was used to transform the data to normal distribution. Tukey and Box-Plot methods were used to exclude the outliers. Nonparametric method was used to establish the reference intervals following the EP28-A3c guideline. Pearson correlation was used to evaluate the correlation between hormone levels and age, while Mann-Whitney U test was employed for quantification of concentration differences on the people who are younger and older than 50 years old. Reference intervals were 0.66-4.95 mIU/L (TSH), 8.97-14.71 pmol/L (FT4), 3.75-5.81 pmol/L (FT3), 73.45-138.93 nmol/L (total T4), and 1.24-2.18 nmol/L (total T3) in male; conversely, reference intervals for female were 0.72-5.84 mIU/L (TSH), 8.62-14.35 pmol/L (FT4), 3.59-5.56 pmol/L (FT3), 73.45-138.93 nmol/L (total T4), and 1.20-2.10 nmol/L (total T3). FT4, FT3, and total T3 levels in male and FT4 level in female had an inverse correlation with age. Total T4 and TSH levels in female were directly correlated. Significant differences in these hormones were also found between younger and older than 50 years old except FT3 in female. Indirect method can be applied for establishment of reference intervals for TSH, FT4, FT3, total T4, and total T3. The reference intervals are narrower than those previously established. Age factor should also be considered. © 2016 Wiley Periodicals, Inc.

  14. Cactus (Opuntia ficus indica f. inermis) fruit juice protects against ...

    African Journals Online (AJOL)



    Dec 18, 2013 ... (MCHC), platelet (Plt) and white blood cell number (WBC)) using a hematology analyzer Coulter MAXM (Beckman Coulter, Inc.,. Fullerton, USA). Evaluation of biochemical parameters. Serum samples were obtained by the centrifugation of the blood collected in the dry tubes at 1000 x g for 10 min at 4°C, ...


    African Journals Online (AJOL)



    Apr 22, 2015 ... Department of Industrial Chemistry, University of Ilorin. Abstract. The need to ... Quantity and quality are the main objectives of tomato ... UV-Vis spectrophotometer. Beckman Coulter, at the Chemistry. Department University of Ilorin. Infra-red. (IR) spectra was recorded on SHIMADZU. 8400s FTIR (Fourier ...

  16. Ultrasound assisted synthesis of PMMA/clay nanocomposites: Study ...

    Indian Academy of Sciences (India)

    specimens of 75 nm by cryoultramicrotome below the glass transition temperature of the sample. The specimens were transferred to a copper grid. The particle diameters were measured in different volume fractions with laser diffraction particle size analyser of model. Beckman Coulter LS 13 320. The mechanical properties ...

  17. Author Details

    African Journals Online (AJOL)

    An evaluation of the Beckman-Coulter AcT3-Part differential haematology analyzer in a tertiary education hospital laboratory. Abstract · Vol 16, No 2 (2007) - Articles Disseminated intravascular coagulopathy in a neonate: management challenges. Abstract · Vol 22, No 3 (2013) - Articles The Clinical and Laboratory Features ...

  18. Download this PDF file

    African Journals Online (AJOL)

    Dr Olaleye

    using Beckman Coulter AC. T diff Analyzer at General hospital, Ekwulobia, Anambra State for the estimation of malaria parasite density. The different species of Plasmodia were identified on the thin Giemsa stained film. The number of parasites in a thick blood film was counted in relation to the number of leukocytes counted ...

  19. Antiproliferation and apoptosis induction of phytic acid in ...

    African Journals Online (AJOL)



    Nov 21, 2011 ... (Beckman Coulter, USA). Detection of apoptotic cell death by flow cytometry. Annexin V-FITC Apoptosis Detection Kit 1 (BD Bioscience) was used to detect early and late apoptotic activity HepG2 cells after 24,. 48 and 72 h of incubation. After treatment of the different forms of phytic acid at concentrations of ...

  20. Nigerian Journal of Medicine - Vol 14, No 2 (2005)

    African Journals Online (AJOL)

    An evaluation of the Beckman-Coulter AcT3-Part differential haematology analyzer in a tertiary education hospital laboratory · EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. HI Biambo, AO Ejele, CA Nwauche, 183-187. ...

  1. Author Details

    African Journals Online (AJOL)

    Biambo, HI. Vol 14, No 2 (2005) - Articles An evaluation of the Beckman-Coulter AcT3-Part differential haematology analyzer in a tertiary education hospital laboratory. Abstract. ISSN: 1115-2613. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL ...

  2. Effect of rifampicin on the kidney of albino rats *1 PETERS, D.E ...

    African Journals Online (AJOL)

    Michael Horsfall

    DOTS (the internationally recommended strategy for. TB control) coverage has increased rapidly from 55 ... (NTBLCP) coordinates and provides strategic direction for TB control activities in Nigeria. The. Federal Ministry ... Switzerland), Spectrophotometer (Beckman Coulter,. DU 520 General Purpose UV / Visual), water bath.

  3. Enough room for Williams and IMF? / Paul Beckman

    Index Scriptorium Estoniae

    Beckman, Paul


    Lõppesid Leedu ja USA energeetikakompanii Williams International läbirääkimised Leedu naftakompleksis osaluse omandamise asjus. IMF uurib Leedu majanduslikku arengut, mida tehing Williamsiga komplitseerib

  4. Plant Guide: Nineleaf biscuitroot (Lomatium triternatum [Pursh] Coulter and Rose) (United States)

    Derek Tilley; Loren St. John; Dan Ogle; Nancy Shaw


    Nineleaf biscuitroot begins growth very early in the spring, often just following snow melt, providing crucial early forage for many wildlife and domestic animals. It is considered one of the most valuable forage species of the genus due to its large stature and high production levels. It is rated as having fair to very good forage for sheep and poor to good forage for...

  5. Expression and sequence characterization of growth hormone ...

    African Journals Online (AJOL)



    Jan 3, 2012 ... Bhd., Malaysia. DTCS Quick start kit (Beckman Coulter) was used for sequencing of the gene. The vectors used for cloning and expression of BbGHBP include pTZ57R/T (Fermentas Inc. USA) and pET22b(+) (Novagen EMD Biosciences, Germany). E. coli host strains used in this study were DH5α and BL21 ...

  6. Apoptosis induced by (di-isopropyloxyphoryl-Trp) 2-Lys-OCH 3 in ...

    Indian Academy of Sciences (India)

    (DIPP-Trp)2-Lys-OCH3 induced a dose-dependent increase of the G2/M cell population in K562 cells, and S cell population in HeLa cells; the sub-G0 population increased dramatically in both cell lines as seen by PI staining experiments using a FACS Calibur Flow cytometer (BeckmanCoulter, USA). Phosphatidylserine ...

  7. The Utilization of Triton X-100 for Enhanced Two-Dimensional Liquid-Phase Proteomics


    Kim, Mina; Lee, Sang-Hee; Min, Jiho; Kobayashi, Fumihisa; Um, Hyun-Ju; Kim, Yang-Hoon


    One of the main challenges in proteomics lies in obtaining a high level of reproducible fractionation of the protein samples. Automated two-dimensional liquid phase fractionation (PF2D) system manufactured by Beckman Coulter provides a process well suited for proteome studies. However, the protein recovery efficiency of such system is low when a protocol recommended by the manufacturer is used for metaproteome profiling of environmental sample. In search of an alternative method that can over...

  8. European Multicenter Study on Analytical Performance of DxN Veris System HCV Assay


    Braun, Patrick; Delgado, Rafael; Drago, Monica; Fanti, Diana; Fleury, Hervé; Gismondo, Maria Rita; Hofmann, Jörg; Izopet, Jacques; Kühn, Sebastian; Lombardi, Alessandra; Marcos, Maria Angeles; Sauné, Karine; O'Shea, Siobhan; Pérez-Rivilla, Alfredo; Ramble, John


    The analytical performance of the Veris HCV Assay for use on the new and fully automated Beckman Coulter DxN Veris Molecular Diagnostics System (DxN Veris System) was evaluated at 10 European virology laboratories. Precision, analytical sensitivity, specificity, and performance with negative samples, linearity, and performance with hepatitis C virus (HCV) genotypes were evaluated. Precision for all sites showed a standard deviation (SD) of 0.22 log10 IU/ml or lower for each level tested. Anal...

  9. Investigation of cholesterol bias due to a matrix effect of external quality assurance samples: how true is your cholesterol method? (United States)

    Pretorius, Carel J; Klingberg, Sandra; Johnson, Leslie; Park, Rodney; Wilgen, Urs; Ungerer, Jacobus P J


    Comparability of cholesterol measurement is clinically required and external quality assurance (EQA) programmes are important to verify the trueness of routine methods. We developed a gas chromatography-isotope dilution mass spectrometry (GC-IDMS) total cholesterol assay to investigate the cause of a suspected matrix-related negative bias with the Beckman Coulter enzymatic method discovered in an EQA programme. The GC-IDMS method was calibrated with certified reference material and verified against a secondary reference method. Bias between the GC-IDMS and Beckman Coulter methods was estimated according to Clinical and Laboratory Standards Institute (CLSI) protocol EP9-A2 with 40 clinical samples. At clinically important decision levels, no significant bias was demonstrated on patients' samples (all results within a ±3% limit). A matrix effect confined to the EQA material that affected the Beckman Coulter total cholesterol method was confirmed. The GC-IDMS method is suitable as a higher order total cholesterol method in a routine clinical laboratory. Matrix effects defeat the objectives of EQA schemes by preventing the verification of trueness. Given the importance of obtaining a true cholesterol result without systematic error, we recommend that EQA material without matrix effects should be used.

  10. Força de tração e volume de solo mobilizado por haste sulcadora em semeadura direta sobre campo nativo, em função do teor de água no solo, profundidade e velocidade de operação Draft and soil loosening by knife type coulter related to soil moisture and planter's working speed and depth

    Directory of Open Access Journals (Sweden)

    Carla T. C. Cepik


    's coulters. The specific aims of the present work are to determine the knowledge of coulter draft requirements and the amount of displaced soil and tractor slippage on a Paleudult soil at different soil moisture condition, in direct planting on natural pastures chemically killed. Draft requirements increased with an increase in working depth. The increase of the ground speed had no effect on draft when soil was dry or moist; it increased however, on friable soil. Soil displacement was always higher with dry soil and at 12 cm working depth. When soil was friable or dry, the front wheels traction was needed to keep tractor slippage within acceptable levels.

  11. Filling in the gaps with non-standard body fluids. (United States)

    Lo, Sheng-Ying; Saifee, Nabiha H; Mason, Brook O; Greene, Dina N


    Body fluid specimens other than serum, plasma or urine are generally not validated by manufacturers, but analysis of these non-standard fluids can be important for clinical diagnosis and management. Laboratories, therefore, rely on the published literature to better understand the validation and implementation of such tests. This study utilized a data-driven approach to determine the clinical reportable range for 11 analytes, evaluated a total bilirubin assay, and assessed interferences from hemolysis, icterus, and lipemia in non-standard fluids. Historical measurements in non-standard body fluids run on a Beckman Coulter DxC800 were used to optimize population-specific clinical reportable ranges for albumin, amylase, creatinine, glucose, lactate dehydrogenase, lipase, total bilirubin, total cholesterol, total protein, triglyceride and urea nitrogen run on the Beckman Coulter AU680. For these 11 analytes, interference studies were performed by spiking hemolysate, bilirubin, or Intralipid® into abnormal serous fluids. Precision, accuracy, linearity, and stability of total bilirubin in non-standard fluids was evaluated on the Beckman Coulter AU680 analyzer. The historical non-standard fluid results indicated that in order to report a numeric result, 4 assays required no dilution, 5 assays required onboard dilutions and 2 assays required both onboard and manual dilutions. The AU680 total bilirubin assay is suitable for clinical testing of non-standard fluids. Interference studies revealed that of the 11 total AU680 analyte measurements on non-standard fluids, lipemia affected 1, icterus affected 3, and hemolysis affected 5. Chemistry analytes measured on the AU680 demonstrate acceptable analytical performance for non-standard fluids. Common endogenous interference from lipemia, icterus, and hemolysis (LIH) are observed and flagging rules based on LIH indices were developed to help improve the clinical interpretation of results.

  12. The Beckman-Quarles theorem for continuous mappings from R^n to C^n


    Tyszka, Apoloniusz


    Let \\phi((x_1,...,x_n),(y_1,...,y_n))=(x_1-y_1)^2+...+(x_n-y_n)^2. We say that f:R^n -> C^n preserves distance d>=0 if for each x,y \\in R^n \\phi(x,y)=d^2 implies \\phi(f(x),f(y))=d^2. We prove that if x,y \\in R^n (n>=3) and |x-y|=(\\sqrt{2+2/n})^k \\cdot (2/n)^l (k,l are non-negative integers) then there exists a finite set {x,y} \\subseteq S(x,y) \\subseteq R^n such that each unit-distance preserving mapping from S(x,y) to C^n preserves the distance between x and y. It implies that each continuou...

  13. [Validation of plasma creatinine measurement on UniCel DxC 600 according to LAB GTA 04 recommendation]. (United States)

    Chianea, Denis; Renard, Christophe; Garcia, Carine; Mbongo, Elvire; Monpeurt, Corine; Vest, Philippe


    The accreditation process, according to NF EN ISO 15189, implies a prior evaluation of the new reagent on-site for the implementation of each new assay technique. Thus, our new standardized method for determination of creatinine (non compensated method) in plasma or serum on UniCel DxC 600 (Beckman Coulter) has been tested according to LAB GTA 04 protocol. The reagent meets the quality criteria recommended by Valtec protocol, except fidelity with the low concentration standard (50 micromol/L). Besides there is no problem of results transferability with the two other techniques used in the laboratory (Jaffe compensated and enzymatic methods performed on Cobas Integra 800).

  14. European Multicenter Study on Analytical Performance of Veris HIV-1 Assay


    Braun, Patrick; Delgado, Rafael; Drago, Monica; Fanti, Diana; Fleury, Hervé; Hofmann, Jörg; Izopet, Jacques; Kalus, Ulrich; Lombardi, Alessandra; Marcos, Maria Angeles; Mileto, Davide; Sauné, Karine; O'Shea, Siobhan; Pérez-Rivilla, Alfredo; Ramble, John


    The analytical performance of the Veris HIV-1 assay for use on the new, fully automated Beckman Coulter DxN Veris molecular diagnostics system was evaluated at 10 European virology laboratories. The precision, analytical sensitivity, performance with negative samples, linearity, and performance with HIV-1 groups/subtypes were evaluated. The precision for the 1-ml assay showed a standard deviation (SD) of 0.14 log10 copies/ml or less and a coefficient of variation (CV) of ≤6.1% for each level ...

  15. Particle size and shape analysis using light scattering, Coulter principle, and image analysis

    International Nuclear Information System (INIS)

    Xu, R.; Di Guida, A.


    Particle size and shape analyses have become important tools for research and applications in a broad spectrum of industries such as pharmaceuticals, metallurgic, ceramics, food and beverage, plastics, petrochemical, clinical, etc. Two of the most utilized technologies for sizing particulate materials are laser diffraction (LD) and electrical sensing zone (ESZ) methods. In a LD experiment, particle size distribution is retrieved from the measured scattering intensity as a function of scattering angle and light wavelength based on the assumption that all particles are spheres. In ESZ measurement, the volume of each individual particle is determined by the change in the resistance of electrolyte while particles flow through an orifice to which a voltage is applied; the particle size distribution is then deduced based on a spherical assumption. Lately, another powerful means for characterizing particles, dynamic image analysis (DIA), has been shown to be able to provide both size and shape information of particulate materials. For real industrial materials, rarely are particles spheres. The effect of non-sphericity in different technologies varies causing discrepancies in results and bias from true characteristics of the sample. Systematic studies of shape effects in particle characterization are few. In this study, samples consisting of particles of the same regular shape are studied using the three technologies (LD, ESZ, and DIA). General conclusions regarding the bias, resolution, reproducibility, and predicted discrepancies from measurements using these technologies are deduced to provide a useful guideline for practical applications of these popular technologies to non-spherical samples


    Directory of Open Access Journals (Sweden)

    I. V. Kudryavtsev


    Full Text Available The article deals with applications of six-color flow analysis for studying the immune state parameters by means of flow cytometry. Whole blood from healthy donors was stained with combination of monoclonal antibodies, i.e., HLA-DR-FITC, CD16+56-PE, CD4-ECD, CD19-ECD, CD8-PC5.5, CD3-PC7, CD45-APC (Beckman Coulter, USA using a “no-wash” technology. To adjust the photomultiplier (PMT voltage, we used the tubes with each of the tested monoclonal antibodies, PMT voltage was considered optimal when the negative population was located in the middle of the first decade at the logarithmic scale. The compensatory adjustment was performed in automatic mode, using Navios software (Beckman Coulter, USA. We discuss an optimal gating strategy in order to assess the populations of interest: total T cells (CD3+CD19-, T helper cells (CD3+CD4+, cytotoxic T lymphocytes (CD3+CD8+, B cells (CD3-CD19+, NK cells (CD3-CD16+CD56+, NKT cells (CD3+CD16+CD56+, activated T lymphocytes (CD3+HLA-DR+. 

  17. [Nephelometry or turbidimetry for the determination of albumin, ApoA, CRP, haptoglobin, IgM and transthyretin: which choice?]. (United States)

    Thuillier, F; Demarquilly, C; Szymanowicz, A; Gaillard, C; Boniface, M; Braidy, C; Daunizeau, A; Gascht, D; Gruson, A; Lagabrielle, J-F; Lasnier, E; Lemdani, M; Macchi, V; Poulin, G; de Sainte Hermine, C; Sancho, J; Schellenberg, F; Sevin, O; Vaubourdolle, M


    Nephelometry, which is considered as the reference method for serum proteins determination requires a specific equipment. The majority of protein determinations are therefore carried out on biochemistry automats using turbidimetry. The objective of a CNBH group (Collège national de biochimie des hôpitaux) was to compare nephelometry and turbidimetry for 7 automats: 2 nephelometers, the BN Prospec (Dade-Behring) and Immage (Beckman-Coulter) and 5 biochemistry systems using turbidimetry, the Integra and Modular (Roche Diagnostics), the LX20 (Beckman-Coulter), RXL (Dade-Behring) and AU (Olympus). The study was based on the determination of sera collections (albumin, ApoA, CRP, haptoglobin, IgM, transthyretin) of 140 samples each: 110 limpid samples and 30 samples called HLI (hemolytic, lipemic or icteric). Fifteen hospitals took part to this work. An ANOVA analysis on limpid samples and quality control sera concluded to an "automat" effect for the 6 tested proteins but did not show a "method" effect, (i.e. nephelometry versus turbidimetry). On the other hand, the transferability of the results was expected to be better and an effort on the choice of the antibodies and the standardization procedures should be made.

  18. A European multicenter study on the analytical performance of the VERIS HBV assay. (United States)

    Braun, Patrick; Delgado, Rafael; Drago, Monica; Fanti, Diana; Fleury, Hervé; Izopet, Jacques; Lombardi, Alessandra; Mancon, Alessandro; Marcos, Maria Angeles; Sauné, Karine; O Shea, Siobhan; Pérez-Rivilla, Alfredo; Ramble, John; Trimoulet, Pascale; Vila, Jordi; Whittaker, Duncan; Artus, Alain; Rhodes, Daniel

    Hepatitis B viral load monitoring is an essential part of managing patients with chronic Hepatits B infection. Beckman Coulter has developed the VERIS HBV Assay for use on the fully automated Beckman Coulter DxN VERIS Molecular Diagnostics System. 1 OBJECTIVES: To evaluate the analytical performance of the VERIS HBV Assay at multiple European virology laboratories. Precision, analytical sensitivity, negative sample performance, linearity and performance with major HBV genotypes/subtypes for the VERIS HBV Assay was evaluated. Precision showed an SD of 0.15 log 10 IU/mL or less for each level tested. Analytical sensitivity determined by probit analysis was between 6.8-8.0 IU/mL. Clinical specificity on 90 unique patient samples was 100.0%. Performance with 754 negative samples demonstrated 100.0% not detected results, and a carryover study showed no cross contamination. Linearity using clinical samples was shown from 1.23-8.23 log 10 IU/mL and the assay detected and showed linearity with major HBV genotypes/subtypes. The VERIS HBV Assay demonstrated comparable analytical performance to other currently marketed assays for HBV DNA monitoring. Copyright © 2017 Elsevier B.V. All rights reserved.

  19. Age-related decline in AMH is assay dependent limiting clinical interpretation of repeat AMH measures across the reproductive lifespan. (United States)

    Plociennik, Lukasz; Nelson, Scott M; Lukaszuk, Aron; Kunicki, Michal; Podfigurna, Agnieszka; Meczekalski, Blazej; Lukaszuk, Krzysztof


    The aim of the study was to determine whether the assays exhibit an interaction with age and exhibit heterogeneous age related declines in AMH. Apart of chronological age, AMH variation was investigated with relation to menstrual cycle day (MCD). The goal implicates two questions: Are distributions of AMH concentrations homogenous after adjustment for the specific AMH assay? Does age-assay product has an effect on AMH depletion? The study was conducted by examining results of AMH tests performed for 12,917 women with four types of AMH assays: Immunotech I generation kit (IMI, 4016 samples), Beckman Coulter II generation kit RUO (BCII RUO, 3430 samples), Beckman Coulter II generation kit with IVD certificate (BCII IVD, 830 samples), and Ansh Labs I generation kit (AnshLabs, 4641 samples). Statistical analysis included ACNOVA and least square regression technique. Menstrual cycle day has no effect on AMH measurements. On the other hand, AMH values differed substantially between the four assays, with a marked discordance in the rate of age-related AMH decline for the four assays (ranging from -8.16% (95% CI: -8.79, -7.54) to -11.53% (95% CI -12.20, -10.87), with a significant interaction between age and assay. (1) The distribution of AMH concentration is heterogeneous after controlling the age across assays; (2) the rate of AMH decline as a function of age is different for the four manual AMH ELISA assays.

  20. Challenges in harmonizing integrated healthcare network laboratories: multi-center evaluation of the hCG5 assay. (United States)

    Greene, Dina N; Baird, Geoffrey S; Kwong, Shiu-Land; Lorey, Thomas S; Holmes, Daniel T


    Beckman Coulter recently introduced a new hCG assay manufactured for the Access 2 and DxI platforms. This assay is the first to use the 5th International Standard (5th IS) as its primary calibration material. Clinical laboratories are required to validate the method performance before testing and reporting patient results. Beckman Coulter Access 2 instruments (n=41) across Kaiser Permanente Northern California were evaluated for their performance characteristics using the hCG5 reagent. Precision, linearity, dilution verification, and patient sample comparisons were performed on each instrument. The assay was linear up to 1350IU/L. Intra-day and inter-day precision ranged from 1.0%-3.3% and 1.8-7.3%, respectively, for the low QC material (mean concentration 4.6IU/L). Percent bias between the previous assay (hCG2) and the hCG5 assay was 3.2 to 22.7% for hCG concentrations 1000IU/L. On board and manual dilutions agreed within 15% following proper adjustment of the instrument dilution factor. Achieving Access 2 inter-instrument agreement on specimens needing dilutions (hCG>1350IU/L) requires validation of the on board dilution factor. Laboratories should use QC material above the linear range to monitor instrument dilution accuracy and precision. Copyright © 2015 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  1. [Clinical evaluation of leukocyte differential count in peripheral blood by five-color flow cytometry]. (United States)

    Xing, Ying; Wang, Jian-zhong; Pu, Cheng-wei; Shang, Ke


    To explore the clinical application values of five-color flow cytometry for leukocyte differential count in peripheral blood. Leukocyte differentiation in 265 peripheral blood samples collected at Peking University First Hospital from September 2010 to December 2010 was detected by standard microscopic cytology as a reference method. Meanwhile, Beckman-Coulter LH750 hematology analyzer and FC500 flow cytometer were performed. Then the correlations were analyzed between microscopic cytology, hematology analyzer and flow cytometry. Forty blood samples collected at Peking University First Hospital, Beijing Daopei Hospital and General Hospital of Beijing Military Command from August 2010 to November 2010 were analyzed by standard microscopic cytology, Beckman-Coulter LH750 hematology analyzer and NAVIOS flow cytometer. Then the correlations between microscopy, hematology analyzer and flow cytometry were explored to analyze the clinical diagnostic efficiency of flow cytometry. Correlation of leukocyte differential count between FC500 flow cytometer and standard microscopic cytology was significant (all P leukocyte differential count between NAVIOS flow cytometer via manual gate and standard microscopic cytology was significant (r > 0.700, all P leukocyte differential count in peripheral blood with different flow cytometers, and the sensitivity of detecting blasts and immature granulocytes is very excellent.

  2. Effects of bacteria and yeast on WBC counting in three automated hematology counters. (United States)

    Kim, Hye Ryoun; Park, Bo Rae G; Lee, Mi Kyung


    Bacteria or yeast may be observed on peripheral blood smears and may lead to spuriously elevated platelet counts. They have been reported to disturb the white blood cell (WBC) differential count if they clumped together, and a large number of such microorganisms have been shown to increase WBC counts. The purpose of this study was to evaluate the spurious rise in WBC counts according to species of microorganisms and automated hematology analyzers. The species we selected were Staphylococcus aureus, Escherichia coli, Candida albicans, C. tropicalis, C. krusei, C. dubliniensis, C. glabrata, and C. parapsilosis. We investigated the effects of bacteria and yeast on peripheral blood samples by the ADVIA 120/2120 Hematology System, Sysmex XE-2100 (TOA Medical Electronics, Kobe, Japan) and Coulter LH 750 (Beckman Coulter, Miami, FL, USA). C. albicans, C. tropicalis, C. krusei, and C. dubliniensis had an overt effect on the WBC count at concentrations of up to 1-5 x 10(7) colony-forming units (CFU)/mL in three automated cell counters, and C. glabrata and C. parapsilosis, when present at concentrations of 1-5 x 10(8) CFU/mL, caused a significant increase in the WBC count obtained by the Sysmex XE-2100 but not by the ADVIA 120/2120 system and Coulter LH 750 (p bacteria and yeast may give erroneously high WBC counts and differential leukocyte counts and should review the peripheral blood smear by microscopy.

  3. Effects of altitude to blood parameters

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    Đelkapić Milosava


    Full Text Available Reducing of partial pressure of oxygen in the air leads to a reduced arterial oxygen saturation and increases secretion of erythropoietin, wich stimulates erythropoiesis. Study included 63 healthy children aged 7 years, devided into 3 groups. I group consists of 21 children from suburb of altitude of 370 m, II group of 22 children from the village on 822 m, III group of 21 children from the town on 411 m. Complete blood count was determined on a Hematology analyzer HmX ( Beckman Coulter. Statistical analysis of data showed that children from II group have a higher average values of erythrocytes than children from the I (p0.05. Results show that stay in the village is useful for stimulation of erythropoiesis.

  4. Within-laboratory and between-laboratory variability in the measurement of anti-müllerian hormone determined within an external quality assurance scheme. (United States)

    Zuvela, Emily; Walls, Melanie; Matson, Phillip


    Ten laboratories in an external quality assurance scheme used the same assay to measure anti-müllerian hormone concentration (Beckman Coulter Gen II) and received twenty serum samples distributed over a 15 month period. The mean bias for all results was only -0.089%, but there was large coefficient of repeatability of 38.8% (sample bias ranged from -37.9% to +54.7%). While each laboratory showed good reproducibility, there was a wide range of average values relative to the consensus value from -24.0% to +22.7%. This between-laboratory variability suggests clinicians should use the same laboratory to avoid problems with result interpretation. Copyright © 2013 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

  5. An extended leukocyte differential count (16 types of circulating leukocytes) using the CytoDiff flow cytometric system can provide information for the discrimination of sepsis severity and prediction of outcome in sepsis patients. (United States)

    Park, Sang Hyuk; Park, Borae G; Park, Chan-Jeoung; Kim, Sue; Kim, Duck-Hee; Jang, Seongsoo; Hong, Suk-Kyung; Chi, Hyun-Sook


    The Beckman Coulter CytoDiff flow cytometric system (Beckman Coulter, Miami, FL) was recently developed for performing leukocyte differential counts in up to 16 leukocyte subpopulations. We compared these leukocyte subpopulation levels among patients with three stages of sepsis (uncomplicated sepsis, severe sepsis, septic shock), especially focused on the discrimination of complicated sepsis from uncomplicated sepsis. We examined a total of 181 samples with sepsis who were admitted to the surgical intensive care unit. In addition, we examined samples obtained from 60 normal healthy volunteers. Both the proportions and absolute numbers of each cell type in the four groups were obtained using the CytoDiff flow cytometric system and compared. Mature neutrophils and immature granulocytes failed to discriminate patients with complicated sepsis from those with uncomplicated sepsis although their absolute numbers were increased compared with normal controls. In contrast, almost all lymphocyte subpopulations and CD16(-) monocytes decreased significantly in patients with complicated sepsis compared with uncomplicated sepsis. Among them, only B lymphocytes showed independent ability to discriminate two groups. Both B lymphocytes and CD16(-) monocytes possessed a significant adverse prognostic impact on overall survival when their absolute numbers decreased. Almost all lymphocyte subpopulations and CD16(-) monocytes decrease in size with increasing sepsis severity. Among them, only B lymphocytes showed independent ability to discriminate patients with complicated sepsis from those with uncomplicated sepsis. Both B lymphocytes and CD16 (-) monocytes show a significant adverse prognostic impact on overall survival outcomes in sepsis patients when their absolute numbers are decreased. Copyright © 2013 Clinical Cytometry Society.

  6. Two new automated, compared with two enzyme-linked immunosorbent, antimüllerian hormone assays. (United States)

    Nelson, Scott M; Pastuszek, Ewa; Kloss, Grzegorz; Malinowska, Iwona; Liss, Joanna; Lukaszuk, Aron; Plociennik, Lukasz; Lukaszuk, Krzysztof


    To compare new automated antimüllerian hormone (AMH) assay performance characteristics from the new automated Elecsys AMH (Roche; Elecsys) and Access AMH (Beckman Coulter; Access) assays with the existing AMH Gen II ELISA (enzyme-linked immunosorbent assay; Gen II; Beckman Coulter) and AMH ELISA (Ansh Labs) assays. Prospective assay evaluation. University-affiliated clinical chemistry laboratory. Patients referred for serum AMH measurement (n = 83) before start of in vitro fertilization cycle between September 2014 and October 2014. None. Serum AMH concentration. Intra-assay coefficients of variation were low; Ansh ≤ 9.0%; Gen II ≤ 5.8%; Access ≤ 10.7%; and Elecsys ≤ 2.8%. The Passing-Bablok regression equations (pmol/L) were y (Access) = 0.128 + (0.781 × Gen II); and y (Access) = 0.302 + (0.742 x Ansh). For y (Elecys) = 0.087 + (0.729 x Gen II) and y (Elecys) = 0.253 + (0.688 x Ansh Labs). For y (Elecys) = 0.943 - (0.037 × Access). For all the assays, AMH exhibited a moderate positive correlation with AFC (r = 0.62-0.64); number of cumulus oocyte complexes (r = 0.60-0.64); and metaphase II oocytes (r = 0.48-0.50). Accuracy of pregnancy prediction, as determined by area under the receiver operating characteristic curve, was uniformly low for all assays (0.62-0.63). The novel automated assays exhibit strong concordance in calibration, but derived values are substantially lower than those obtained from pre-existing assays, with assay-specific interpretation required for routine clinical use. These results highlight the need for an international standard of measurement of AMH. Copyright © 2015 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  7. Validation of methods performance for routine biochemistry analytes at Cobas 6000 analyzer series module c501. (United States)

    Supak Smolcic, Vesna; Bilic-Zulle, Lidija; Fisic, Elizabeta


    Cobas 6000 (Roche, Germany) is biochemistry analyzer for spectrophotometric, immunoturbidimetric and ion-selective determination of biochemical analytes. Hereby we present analytical validation with emphasis on method performance judgment for routine operation. Validation was made for 30 analytes (metabolites, enzymes, trace elements, specific proteins and electrolytes). Research included determination of within-run (N = 20) and between-run imprecision (N = 30), inaccuracy (N = 30) and method comparison with routine analyzer (Beckman Coulter AU640) (N = 50). For validation of complete analytical process we calculated total error (TE). Results were judged according to quality specification criteria given by European Working Group. Within-run imprecision CVs were all below 5% except for cholesterol, triglycerides, IgA and IgM. Between-run CVs for all analytes were below 10%. Analytes that did not meet the required specifications for imprecision were: total protein, albumin, calcium, sodium, chloride, immunoglobulins and HDL cholesterol. Analytes that did not fulfill requirements for inaccuracy were: total protein, calcium, sodium and chloride. Analytes that deviated from quality specifications for total error were: total protein, albumin, calcium, sodium, chloride and IgM. Passing-Bablok regression analysis provided linear equation and 95% confidence interval for intercept and slope. Complete accordance with routine analyzer Beckman Coulter AU640 showed small number of analytes. Other analytes showed small proportional and/or small constant difference and therefore need to be adjusted for routine operation. Regarding low CV values, tested analyzer has satisfactory accuracy and precision and is extremely stable. Except for analytes that are coherent on both analyzers, some analytes require adjustments of slope and intercept for complete accordance.

  8. Challenges in harmonizing integrated healthcare network laboratories: Multi-center evaluation of the AccuTnI+3 troponin assay. (United States)

    Greene, Dina N; Holmes, Daniel T; Liang, Joy; Kwong, Shiu-Land; Lorey, Thomas S; Petrie, Matthew S


    Beckman Coulter has recently introduced a new troponin assay manufactured for the Access2 and DxI platforms, releasing it under the name AccuTnI+3. Clinical laboratories are required to validate method performance before testing and reporting patient results. Beckman Coulter Access 2 instruments (n=42) across Kaiser Permanente Northern California were evaluated for their performance characteristics using the AccuTnI+3 reagent. Precision, linearity, and patient sample comparisons were performed on each instrument. Limit of the blank (LOB), limit of detection (LOD), limit of quantitation (LOQ), serum plasma comparisons, and specimen stability were evaluated using a single instrument. The assay was linear from 0-100,000ng/L. The LOB, LOD and LOQ were determined to be 5, 8 and 20ng/L, respectively. Interday precision on the low QC (mean concentration 41ng/L) ranged from 3.0% to 14.2%. The bias observed between the former assay (AccuTnI) and the AccuTnI+3 was comparable to the inter-instrument bias for either assay. Non-uniform distribution was observed in the precision and inter-instrument/inter-assay comparisons among the instruments evaluated. The AccuTnI and AccuTnI+3 troponin assays are equivalent across the analytical measuring range. There was no significant difference at the medical decision point. No changes in patient results are anticipated. However, the assay-independent inter-instrument bias observed is an important consideration for harmonization efforts. Copyright © 2014. Published by Elsevier Inc.

  9. An extended leukocyte differential count (16 types of circulating leukocytes) using the cytodiff flow cytometric system can provide informations for the discrimination of sepsis severity and prediction of outcome in sepsis patients. (United States)

    Park, Sang Hyuk; Park, Borae G; Park, Chan-Jeoung; Kim, Sue; Kim, Duck-Hee; Jang, Seongsoo; Hong, Suk-Kyung; Chi, Hyun-Sook


    Background: The Beckman Coulter CytoDiff flow cytometric system (Beckman Coulter, Miami, FL, USA) was recently developed for performing leukocyte differential counts in up to 16 leukocyte subpopulations. We compared these leukocyte subpopulation levels among patients with three stages of sepsis (uncomplicated sepsis, severe sepsis, septic shock), especially focused on the discrimination of complicated sepsis from uncomplicated sepsis. Methods: We examined a total of 181 samples with sepsis who were admitted to the surgical intensive care unit. In addition, we examined samples obtained from 60 normal healthy volunteers. Both the proportions and absolute numbers of each cell type in the four groups were obtained using the CytoDiff flow cytometric system and compared. Results: Mature neutrophils and immature granulocytes failed to discriminate patients with complicated sepsis from those with uncomplicated sepsis although their absolute numbers were increased compared with normal controls. In contrast, almost all lymphocyte subpopulations and CD16(-) monocytes decreased significantly in patients with complicated sepsis compared with uncomplicated sepsis. Among them, only B lymphocytes showed independent ability to discriminate two groups. Both B lymphocytes and CD16(-) monocytes possessed a significant adverse prognostic impact on overall survival when their absolute numbers decreased. Conclusions: Almost all lymphocyte subpopulations and CD16(-) monocytes decrease in size with increasing sepsis severity. Among them, only B lymphocytes showed independent ability to discriminate patients with complicated sepsis from those with uncomplicated sepsis. Both B lymphocytes and CD16(-) monocytes show a significant adverse prognostic impact on overall survival outcomes in sepsis patients when their absolute numbers are decreased. © 2013 Clinical Cytometry Society. Copyright © 2013 Clinical Cytometry Society.

  10. [Hematologic indices in different age wistar rats, receiving a balanced semi-synthetic vivary diet]. (United States)

    Mustafina, O K; Trushina, É N; Shumakova, E A; Arianova, E A; Tyshko, N V; Pashorina, V A


    This paper presents the results of research of hematologic parameters of male Wistar rats 1, 2, 3, 4 and 6 months age, which received a balanced semisynthetic diet. Studies were carried out at the Hematology analyzer Coulter AC TTM 5 diff OV (Beckman Coulter, USA) with the program, specially developed for the study of rats' blood. According to the results of research, was found a statistically significant increased of the number of red blood cells; the concentration of hemoglobin and hematocrit in animals 2-6 months compared with rats, 1 month age. With age, there is a decrease of the mean corpuscular volume and the mean corpuscular hemoglobin. The number of white blood cells in rats of 2-4 months age are significantly higher than in rats of 1 and 6 months age. The number of neutrophils and eosinophils in rats of to the 2 month are of is lover than once in rats of 1 month age, and increases values in animals of 6 months age. The number of lymphocytes has the highest value in the rat of 2-3 months age and the minimum value is that in animals of 6 months age. With increasing of the age of the animals the reduction of contents of monocytes was noted. The content of platelets and the platelet crit in the blood of rats 6 months age is statistically greater than those in 1-month age animals. The average volume of platelet is the stable index, with age does not change.

  11. A Multicenter Reference Intervals Study for Specific Proteins in China (United States)

    Qin, Xuzhen; Tang, Guodong; Qiu, Ling; Li, Peng Chang; Xia, Liangyu; Chen, Ming; Tao, Zhihua; Li, Shijun; Liu, Min; Wang, Liang; Gao, Shang; Yu, Songlin; Cheng, Xinqi; Han, Jianhua; Hou, Li’an; Kawano, Reo; Ichihara, Kiyoshi


    Abstract A multicenter study conducted in healthy population of 6 cities from the 4 corners and central China for 7 serum-specific proteins to identify the sources of variation and establish the reference intervals on 2 automation platforms. A total of 3148 subjects aged 19 to 64 years old were enrolled in this study to ensure at least 120 participants in each 10-year age group and each city. The majority of samples were transported to central laboratory and measured on both Beckman AU5800 and Immage 800 analytical systems. Three-level nested ANOVA, multiple regression analysis, and the scatter plot were used to explore the variations from sex, age, region, BMI, cigarette smoking, and so on. The latent abnormal value exclusion (LAVE) method was applied at the time of computing RIs as a method for secondary exclusion. Regionality was not observed in any of the immunoassay in China. Variations for sex were significant for IgM among the immune analytes. For CRP and hsCRP results with turbidimetry method (Beckman Coulter AU5800) were lower than the nephelometry method (Beckman Immage). The LAVE method did not affect the RIs computed for the majority of analytes except C4, CRP, and hsCRP. In the scatter plot at the age of 45 years old C3, C4, and IgM reached an inflection point, accordingly RIs were separated by the age group. With the lack of regional differences and the well-standardized status of test results, the RIs of C3, IgG, IgA, IgM derived from this nationwide study can be used for the entire Chinese population. C4, CRP, and hsCRP were affected by different platforms and gender was a significant source of variation for IgM, so they had separated RIs. PMID:26656356

  12. Svante Beckman and Sten Månsson (ed. Kultursverige 2009 - Problemanalys och Kulturstatistik, Linköping: Sörlins Förlag, 2008

    Directory of Open Access Journals (Sweden)

    Arne Ruth


    Full Text Available According to recent European statistics, Swedish cultural policy has succeeded remarkably well in achieving its original objective: to promote popular interest in the Arts. Sweden (closely followed by our Nordic neighbors tops the list when it comes to participation in cultural activities. The figures are included in a body of statistics that, together with commentary by Sten Månsson of the Swedish Cultural Council, make up the greater part of Kultursverige 2009.

  13. Information Technology and Library Management. Festschrift in Honour of Margaret Beckman. Papers Presented at the International Essen Symposium (13th, Essen, West Germany, October 22-25, 1990). (United States)

    Helal, Ahmed H., Ed.; Weiss, Joachim W., Ed.

    The 16 papers in this collection focus on the impact of new technologies on libraries and their managers: (1) "Has Technology Failed Us?" (Frederick W. Lancaster); (2) "The Wheel of Fortune: Academic Libraries. IT and the Re-emerging Past" (Alasdair Paterson); (3) "Management Strategies for Enhancing the Adoption of Technological Innovations"…

  14. Performance verification of the new fully automated Aquios flow cytometer PanLeucogate (PLG platform for CD4-T-lymphocyte enumeration in South Africa.

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    Lindi-Marie Coetzee

    Full Text Available The National Health Laboratory Service (NHLS offers wide-scale CD4 testing through a network of laboratories in South Africa. A new "load and go" cytometer (Aquios CL, Beckman Coulter, developed with a PLG protocol, was validated against the predicate PLG method on the Beckman Coulter FC500 MPL/CellMek platform.Remnant routine EDTA blood CD4 reference results were compared to results from two Aquios/PLG instruments (n = 205 and a further n = 1885 samples tested to assess daily testing capacity. Reproducibility was assessed using ImmunotrolTM and patient samples with low, medium, high CD4 counts. Data was analyzed using GraphPad software for general statistics and Bland-Altman (BA analyses. The percentage similarity (%Sim was used to measure the level of agreement (accuracy of the new platform versus the predicate and variance (%SimCV reported to indicate precision of difference to predicate.205 samples were tested with a CD4 count range of 2-1228 cells/μl (median 365cells/μl. BA analysis revealed an overall -40.5±44.0cells/μl bias (LOA of 126.8 to 45.8cells/μl and %Sim showing good agreement and tight precision to predicate results (94.83±5.39% with %SimCV = 5.69%. Workflow analysis (n = 1885 showed similar outcomes 94.9±8.9% (CV of 9.4% and 120 samples/day capacity. Excellent intra-instrument reproducibility was noted (%Sim 98.7±2.8% and %SimCV of 2.8%. 5-day reproducibility using internal quality control material (Immunotrol™ showed tight precision (reported %CV of 4.69 and 7.62 for Normal and Low material respectively and instrument stability.The Aquios/PLG CD4 testing platform showed clinically acceptable result reporting to existing predicate results, with good system stability and reproducibility with a slight negative but precise bias. This system can replace the faded XL cytometers in low- to medium volume CD4 testing laboratories, using the standardized testing protocol, with better staff utilization especially where technical

  15. Characteristics and Echogenicity of Clinical Ultrasound Contrast Agents: An In Vitro and In Vivo Comparison Study. (United States)

    Hyvelin, Jean-Marc; Gaud, Emmanuel; Costa, Maria; Helbert, Alexandre; Bussat, Philippe; Bettinger, Thierry; Frinking, Peter


    To compare physicochemical characteristics and in vitro and in vivo contrast-enhanced ultrasound imaging performance of 3 commercially available ultrasound contrast agents: SonoVue (Bracco Imaging SpA, Colleretto Giacosa, Italy; also marketed as Lumason in the USA), Definity (Lantheus Medical Imaging, North Billerica, MA) and Optison (GE Healthcare AS, Oslo, Norway). Physicochemical characteristics were measured with a Multisizer Coulter Counter (Beckman Coulter, Fullerton, CA). Two ultrasound systems (Aplio 500; Toshiba Medical Systems Corp, Tochigi-ken, Japan; and Logiq E9; GE Healthcare, Little Chalfont, England) were used with different transducers. Contrast enhancement was measured in vitro by dose-ranging measurements using a custom-built beaker setup; in vivo imaging performances were compared in pigs (heart and liver) and rabbits (liver). Quantitative analyses were performed with VueBox quantification software (Bracco Suisse SA, Plan-les-Ouates, Switzerland). Measured physicochemical characteristics were in agreement with those provided by the manufacturers. In vitro data demonstrated that the performance of SonoVue was similar to or better than that of Definity but superior to Optison (normalized scattered power 2- to 10-fold higher with SonoVue). Similar results were obtained in vivo, although the duration of enhancement in the pig heart was longer for SonoVue compared to Definity, and quantitative analysis revealed higher enhancement for SonoVue (1.5-fold increase). For liver imaging, SonoVue and Definity showed similar contrast enhancement and duration of enhancement, but compared to Optison, both peak enhancement and duration of enhancement were superior for SonoVue (up to 2-fold increase). Imaging performance of SonoVue was similar to or slightly better than that of Definity, but it was superior to Optison for the conditions used in this study. © 2017 by the American Institute of Ultrasound in Medicine.

  16. Multicenter evaluation of an enzymatic method for glycated albumin. (United States)

    Paleari, Renata; Bonetti, Graziella; Callà, Cinzia; Carta, Mariarosa; Ceriotti, Ferruccio; Di Gaetano, Nicola; Ferri, Marilisa; Guerra, Elena; Lavalle, Gabriella; Cascio, Claudia Lo; Martino, Francesca Gabriela; Montagnana, Martina; Moretti, Marco; Santini, Gabriele; Scribano, Donata; Testa, Roberto; Vero, Anna; Mosca, Andrea


    The use of glycated albumin (GA) has been proposed as an additional glycemic control marker particularly useful in intermediate-term monitoring and in situation when HbA 1c test is not reliable. We have performed the first multicenter evaluation of the analytical performance of the enzymatic method quantILab Glycated Albumin assay implemented on the most widely used clinical chemistry analyzers (i.e. Abbott Architect C8000, Beckman Coulter AU 480 and 680, Roche Cobas C6000, Siemens ADVIA 2400 and 2400 XPT). The repeatability of the GA measurement (expressed as CV, %) implemented in the participating centers ranged between 0.9% and 1.2%. The within-laboratory CVs ranged between 1.2% and 1.6%. A good alignment between laboratories was found, with correlation coefficients from 0.996 to 0.998. Linearity was confirmed in the range from 7.6 to 84.7%. The new enzymatic method for glycated albumin evaluated by our investigation is suitable for clinical use. Copyright © 2017 Elsevier B.V. All rights reserved.


    Directory of Open Access Journals (Sweden)

    A. I. Budkova


    Full Text Available Distinct changes of B-cell subpopulations are observed in most systemic rheumatic diseases associated with polyclonal B cell hyperreactivity. Immunosuppressive and cytostatic therapy may also differentially influence B lymphocyte subsets in these. We studied subpopulations of B cells in systemic rheumatic patients along treatment with cytostatics. We analyzed B cell phenotypes in ninety-nine blood samples from the patients with systemic lupus erythematosus (SLE, n = 25, systemic sclerosis (n = 27, Sjogren’s syndrome (n = 47 in the course of their hospital treatment. Control group consisted of 49 healthy blood donors. Phenotyping of blood B-cell subpopulations was performed by means of flow cytometry (Beckman Coulter, USA. Naïve B-cell subpopulations in SLE patients who underwent cyclophosphan treatment, were underrepresented, if compared with normal control group, whereas plasmablast levels were increased irrespectively of medication mode. B cell population exhibits a natural heterogeneity, thus making it necessary to analyze distinct B cell subpopulations as independent functional units, when studying different rheumatic diseases. The levels of plasmablasts which are active antibody producers, remain high, despite immunosuppressive therapy performed in SLE. Thus, therapy targeted against certain B cell subsets, could be able to provide a more effective treatment for the patients with systemic rheumatic diseases.

  18. Relationship of clinical and microbiological variables in patients with type 1 diabetes mellitus and periodontitis. (United States)

    Sakalauskiene, Jurgina; Kubilius, Ricardas; Gleiznys, Alvydas; Vitkauskiene, Astra; Ivanauskiene, Egle; Šaferis, Viktoras


    The aim of the study was to analyze how metabolic control of type 1 diabetes is related to clinical and microbiological periodontal parameters. The study involved 56 subjects aged from 19 to 50 years divided into 2 groups: healthy subjects (the H group), and diabetic (type 1 diabetes) patients with chronic untreated generalized periodontitis (the DM group). The glycosylated hemoglobin value (HbA1c) was determined using the UniCel DxC 800 SYNCHRON System (Beckman Coulter, USA), and the concentration in blood was measured by the turbidimetric immunoinhibition method. A molecular genetic assay (Micro-IDent plus, Germany) was used to detect periodontopathogenic bacteria in plaque samples. Periodontitis was confirmed by clinical and radiological examination. Fusobacterium nucleatum, Capnocytophaga species, and Eikenella corrodens were the most frequently found bacteria in dental plaque samples (77.8%, 66.7%, and 33.4%, respectively), whereas Aggregatibacter actinomycetemcomitans was identified 40.7% less frequently in the DM group than in the H group. The strongest relationship was observed between the presence of 2 periodontal pathogens - F. nucleatum and Capnocytophaga spp. - and poorer metabolic control in type 1 diabetes patients (HbA1c) and all clinical parameters of periodontal pathology. Periodontal disease was more evident in type 1 diabetic patients, and the prevalence of periodontitis was greatly increased in subjects with poorer metabolic control.

  19. Serum biochemistry reference intervals of live wild dugongs (Dugong dugon) from urban coastal Australia. (United States)

    Lanyon, Janet M; Wong, Arthur; Long, Trevor; Woolford, Lucy


    Little is known about the baseline clinical pathology of the dugong (Dugong dugon), a vulnerable marine mammal found in tropical coastal marine systems. The purpose of the study was to collect and determine reference intervals (RI) for select serum biochemical variables for dugongs, and to analyze differences between males and females and different age groups. Reference intervals were established from 103 apparently healthy, wild-caught dugongs for 31 analytes using a Beckman Coulter AU400 Automated Chemistry Analyzer and an Olympus AU680 Chemistry-Immuno Analyzer. Significant differences (P serum sodium, potassium, bicarbonate, glucose, and l-lactate concentrations and higher anion gap, compared to sub-adults. Male dugongs had higher triglyceride and l-lactate concentrations than females. Pregnant females displayed higher l-lactate levels compared to nonpregnant animals. Statistical differences in variables within the population contributed to better understanding of the physiologic differences between cohorts. Some serum biochemistry changes observed in this study here also potentially include some effects of pursuit on dugongs (eg, higher l-lactate); however, as all dugongs were subject to similar capture and handling, serum biochemistry RI should be considered as normal for captured dugongs. The serum biochemical RI documented here are considered representative of a population of healthy captured dugongs. They provide a baseline for health surveillance of this and other dugong populations. © 2015 American Society for Veterinary Clinical Pathology.

  20. Hook effect in Abbott i-STAT β-human chorionic gonadotropin (β-hCG) point of care assay. (United States)

    Wilgen, Urs; Pretorius, Carel J; Gous, Rehna S; Martin, Cameron; Hale, Vincent J; Ungerer, Jacobus P J


    Point-of-care testing for β-hCG has been widely advocated to allow rapid diagnosis/exclusion of pregnancy in the emergency department. A quantitative blood β-hCG assay has the additional benefit of being able to monitor the viability of pregnancy, using serial measurements, to determine the appropriate expected increase in β-hCG levels over time (e.g. ectopic pregnancy), and aiding in determining if an intrauterine gestational sac should be visible on sonographic imaging. Evaluation of the newly released Abbott i-STAT β-hCG point-of-care assay with the Beckman Coulter β-hCG laboratory assay in use. Whole blood, plasma and serum samples with a wide range of β-hCG concentrations were analysed by both methods. The Abbott I-STAT β-hCG compares favourably, can be performed on heparinised whole blood, plasma and serum, and shows acceptable accuracy and precision. However a hook effect at elevated β-hCG was shown in gestational trophoblastic disease as well as normal pregnancies. The i-STAT β-hCG performs acceptably in its intended use in the early detection of pregnancy, but results should always be interpreted within the clinical context, as a hook effect may occur. Crown Copyright © 2014. Published by Elsevier Inc. All rights reserved.

  1. Ultrasonic sonoelastography of scrotum in the diagnosis of male fertility

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    O. B. Zhukov


    Full Text Available Objective: to determine the possibility of using detect the scrotum in assessing reproductive function of men.Materials and methods. The study was conducted in men aged 20–44 years. The first group of men was 25 patients with secretory infertility. The second group included 25 male infertility varicocele. The control group presents 12 men in the age range 22–31 year with the implementation of the reproductive function of 1 or more children. The study was performed by ultrasonic scanner expert class Acuson S2000 manufacture of the company Siemens, was used microwave sensor 18L6 HD. Sex hormones were studied on immunochemiluminescence analyzer Access 2 production Beckman Coulter (USA. Obtaining and study of ejaculate was carried out according to Recommendations of the WHO (5th edition.Results. The application of research shear transverse wave Vs men with secretory infertility and varicocele is accompanied by the increase of its value to 1.5–1.6 m/s and is characterized by 35–45 % from similar parameters of reproductive healthy men. In half of the cases studies such an increase in performance and decrease in the area of mapping the so-called “soft” areas of the epididymis is accompanied by a decrease in the concentration and actively motile sperm. The use of such method of instrumental diagnostics will allow at the stage of preliminary ultrasound screening to suspect a violation of the reproductive capacity of men.

  2. Performance evaluation of Samsung LABGEO(HC10) Hematology Analyzer. (United States)

    Park, Il Joong; Ahn, Sunhyun; Kim, Young In; Kang, Seon Joo; Cho, Sung Ran


    The Samsung LABGEO(HC10) Hematology Analyzer (LABGEO(HC10)) is a recently developed automated hematology analyzer that uses impedance technologies. The analyzer provides 18 parameters including 3-part differential at a maximum rate of 80 samples per hour. To evaluate the performance of the LABGEO(HC10). We evaluated precision, linearity, carryover, and relationship for complete blood cell count parameters between the LABGEO(HC10) and the LH780 (Beckman Coulter Inc) in a university hospital in Korea according to the Clinical and Laboratory Standards Institute guidelines. Sample stability and differences due to the anticoagulant used (K₂EDTA versus K₃EDTA) were also evaluated. The LABGEO(HC10) showed linearity over a wide range and minimal carryover ( 0.92) except for mean corpuscular hemoglobin concentration. The bias estimated was acceptable for all parameters investigated except for monocyte count. Most parameters were stable until 24 hours both at room temperature and at 4°C. The difference by anticoagulant type was statistically insignificant for all parameters except for a few red cell parameters. The accurate results achievable and simplicity of operation make the unit recommendable for small to medium-sized laboratories.

  3. An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems

    Directory of Open Access Journals (Sweden)

    Verschraegen Gerda


    Full Text Available Abstract Background Currently, most laboratories identify yeasts routinely on the basis of morphology and biochemical reactivity. This approach has quite often limited discriminatory power and may require long incubation periods. Due to the increase of fungal infections and due to specific antifungal resistence patterns for different species, accurate and rapid identification has become more important. Several molecular techniques have been described for fast and reliable identification of yeast isolates, but interlaboratory exchangeability of identification schemes of molecular techniques has hardly been studied. Here, we compared amplified ITS2 fragment length determination by an ABI Prism 310 (Applied Biosystems, Foster City, Ca. capillary electrophoresis system with that obtained by a CEQ8000 (Beckman Coulter, Fullerton, Ca. capillary electrophoresis system. Results Although ITS2 size estimations on both systems differed and separate libraries had to be constructed for each system, both approaches had the same discriminatory power with regard to the 44 reference strains, identical identifications were obtained for 39/ 40 clinical isolates in both laboratories and strains from 51 samples were correctly identified using CEQ8000, when compared to phenotypic identification. Conclusion Identification of yeasts with ITS2-PCR followed by fragment analysis can be carried out on different capillary electrophoresis systems with comparable discriminatory power.

  4. A Turkish 3-center study evaluation of serum folic acid and vitamin B12 levels in Alzheimer disease. (United States)

    Ulusu, Nuriye Nuray; Yilmaz, Gökhan; Erbayraktar, Zübeyde; Evlice, Ahmet Turan; Aras, Sevgi; Yener, Görsev; Avci, Aslihan


    Alzheimer disease, a common proteopathy of advanced age, is characterized by cortical atrophy, neuron degeneration, neuronal loss, and accumulation of extracellular amyloid β plaques. We aimed to investigate serum vitamin B12 and folic acid levels in Alzheimer disease and other dementia patients, as a potential screening test to detect presymptomatic Alzheimer disease in Turkish patients. We evaluated folic acid and vitamin B12 levels in Alzheimer disease patients as well as in other dementia and geriatric patients from Ankara, Dokuz Eylül, and Çukurova university hospitals; 290 female and male geriatric subjects were enrolled. Vitamin B12 and folic acid levels were measured using Roche E170 and Beckman Coulter DXI 800 immunoassays (chemiluminescence) according to the manufacturers' guideline in all centers. We evaluated the results of folic acid and vitamin B12 in Alzheimer disease, other dementias and geriatric patients. No significant difference between the groups regarding the routine control of biochemical parameters was observed. Currently, serum folic and vitamin B12 levels are not diagnostically reliable tests for screening presymptomatic Alzheimer disease. However, the results may statistically be significant if we increase the sample size.

  5. Biological Reference Interval for Hematological Profile of Umbilical Cord Blood: A Study Conducted at A Tertiary Care Centre in South India (United States)

    Raj, Reddy S. Sudheer; Priyathersini, N.; Rajendran, Rithika; Rajendran, Rashmika; Ramadoss, Umalakshmi


    Introduction Umbilical cord blood (UCB) a source of hematopoietic stem cells, is also an acceptable sample to assess neonatal sepsis. Though reports are available for stem cell counts very minimal literature is available regarding hematologic parameters, which may vary on ethnicity. Aim To establish biological reference interval for hematological parameters of umbilical cord blood to guide neonatologists, hematopoietic stem cell transplant specialists and future analysis. Materials and Methods Prospective longitudinal study was done from January 2014 to April 2014 after ethics committee approval. UCB from 120 full term new borns of normal birth weight born out of uneventful pregnancy to mothers aged between 21 to 45 years with hemoglobin above 10g/dL were processed in Beckman Coulter LH780 analyzer for complete blood count and counter checked by peripheral smear. Results tabulated in Microsoft excel are analyzed using IBM SPSS statistics 16 software. Results Male to female ratio is 1:1.05. There is no difference in the values between males and females. When compared with few studies available, though many values are comparable a few values are not comparable. Conclusion This study can be a useful guide to neonatologists, hematopoietic stem cells transplant hematologists and future analysis. PMID:26557584

  6. Automated genomic DNA purification options in agricultural applications using MagneSil paramagnetic particles (United States)

    Bitner, Rex M.; Koller, Susan C.


    The automated high throughput purification of genomic DNA form plant materials can be performed using MagneSil paramagnetic particles on the Beckman-Coulter FX, BioMek 2000, and the Tecan Genesis robot. Similar automated methods are available for DNA purifications from animal blood. These methods eliminate organic extractions, lengthy incubations and cumbersome filter plates. The DNA is suitable for applications such as PCR and RAPD analysis. Methods are described for processing traditionally difficult samples such as those containing large amounts of polyphenolics or oils, while still maintaining a high level of DNA purity. The robotic protocols have ben optimized for agricultural applications such as marker assisted breeding, seed-quality testing, and SNP discovery and scoring. In addition to high yield purification of DNA from plant samples or animal blood, the use of Promega's DNA-IQ purification system is also described. This method allows for the purification of a narrow range of DNA regardless of the amount of additional DNA that is present in the initial sample. This simultaneous Isolation and Quantification of DNA allows the DNA to be used directly in applications such as PCR, SNP analysis, and RAPD, without the need for separate quantitation of the DNA.

  7. A European multicientre study on the comparison of HIV-1 viral loads between VERIS HIV-1 Assay and Roche COBAS® TAQMAN® HIV-1 test, Abbott RealTime HIV-1 Assay, and Siemens VERSANT HIV-1 Assay. (United States)

    Braun, Patrick; Delgado, Rafael; Drago, Monica; Fanti, Diana; Fleury, Hervé; Hofmann, Jörg; Izopet, Jacques; Kühn, Sebastian; Lombardi, Alessandra; Mancon, Alessandro; Marcos, Mª Angeles; Mileto, Davide; Sauné, Karine; O'Shea, Siobhan; Pérez-Rivilla, Alfredo; Ramble, John; Trimoulet, Pascale; Vila, Jordi; Whittaker, Duncan; Artus, Alain; Rhodes, Daniel


    Viral load monitoring is essential for patients under treatment for HIV. Beckman Coulter has developed the VERIS HIV-1 Assay for use on the novel, automated DxN VERIS Molecular Diagnostics System. ¥ OBJECTIVES: Evaluation of the clinical performance of the new quantitative VERIS HIV-1 Assay at multiple EU laboratories. Method comparison with the VERIS HIV-1 Assay was performed with 415 specimens at 5 sites tested with COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1 Test, v2.0, 169 specimens at 3 sites tested with RealTime HIV-1 Assay, and 202 specimens from 2 sites tested with VERSANT HIV-1 Assay. Patient monitoring sample results from 4 sites were also compared. Bland-Altman analysis showed the average bias between VERIS HIV-1 Assay and COBAS HIV-1 Test, RealTime HIV-1 Assay, and VERSANT HIV-1 Assay to be 0.28, 0.39, and 0.61 log 10 cp/mL, respectively. Bias at low end levels below 1000cp/mL showed predicted bias to be DxN VERIS System demonstrated comparable clinical performance to COBAS ® HIV-1 Test, RealTime HIV-1 Assay, and VERSANT HIV-1 Assay. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. European Multicenter Study on Analytical Performance of DxN Veris System HCV Assay. (United States)

    Braun, Patrick; Delgado, Rafael; Drago, Monica; Fanti, Diana; Fleury, Hervé; Gismondo, Maria Rita; Hofmann, Jörg; Izopet, Jacques; Kühn, Sebastian; Lombardi, Alessandra; Marcos, Maria Angeles; Sauné, Karine; O'Shea, Siobhan; Pérez-Rivilla, Alfredo; Ramble, John; Trimoulet, Pascale; Vila, Jordi; Whittaker, Duncan; Artus, Alain; Rhodes, Daniel W


    The analytical performance of the Veris HCV Assay for use on the new and fully automated Beckman Coulter DxN Veris Molecular Diagnostics System (DxN Veris System) was evaluated at 10 European virology laboratories. Precision, analytical sensitivity, specificity, and performance with negative samples, linearity, and performance with hepatitis C virus (HCV) genotypes were evaluated. Precision for all sites showed a standard deviation (SD) of 0.22 log 10 IU/ml or lower for each level tested. Analytical sensitivity determined by probit analysis was between 6.2 and 9.0 IU/ml. Specificity on 94 unique patient samples was 100%, and performance with 1,089 negative samples demonstrated 100% not-detected results. Linearity using patient samples was shown from 1.34 to 6.94 log 10 IU/ml. The assay demonstrated linearity upon dilution with all HCV genotypes. The Veris HCV Assay demonstrated an analytical performance comparable to that of currently marketed HCV assays when tested across multiple European sites. Copyright © 2017 American Society for Microbiology.

  9. Normal lymphocyte immunophenotype in an elderly population

    Directory of Open Access Journals (Sweden)

    Sâmia Macedo Queiroz Mota Castellão Tavares


    Full Text Available OBJECTIVE: The aim of this work was to evaluate the lymphocyte immunophenotype in an elderly population.METHODS: This study enrolled 35 over 60-year-old volunteers and a control group composed of 35 young adults. The study included elderly without diseases that might affect the functioning of the immune system. These individuals were consulted by doctors and after a physical examination, laboratory tests were performed using a Beckman Coulter (r flow cytometer. The GraphPad Prism computer program was employed for statistical analysis with the level of significance being set for p-values <0.05.RESULTS: There is a statistically significant reduction in the number of lymphocytes (CD8 +, CD2 + and CD3 + cells in the elderly compared to young adults. These low rates are explained by changes attributed to aging and may be partly responsible for the reduction in the cellular immune response, lower proliferative activity and the low cytotoxicity of lymphocytes.CONCLUSION: These parameters showed greater impairment of adaptive immunity in the elderly population and can therefore explain the greater fragility of the aged body to developing diseases.

  10. Commercial reference shape standards use in the study of particle shape effect on laser diffraction particle size analysis. (United States)

    Kelly, Richard N; Kazanjian, Jacqueline


    The purpose of this paper is to describe the use of LGC Promochem AEA 1001 to AEA 1003 monosized fiber-analog shape standards in the study of the effect of particle shape on laser diffraction (LD) particle size analysis (psa). The psa of the AEA standards was conducted using LD psa systems from Beckman Coulter, Horiba, and Malvern Instruments. Flow speed settings, sample refractive index values, and sample cell types were varied to examine the extent to which the shape effect on LD psa results is modified by these variables. The volume and number probability plots resulting from these measurements were each characterized by a spread in the particle size distribution that roughly extended from the breadth to the longest dimension of the particles. For most of the selected sample refractive index values, the volume probability plots were characterized by apparent bimodal distributions. The results, therefore, provide experimental verification of the conclusions from theoretical studies of LD psa system response to monosized elliptical particles in which this apparent bimodality was the predicted result in the case of flow-oriented particles. The data support the findings from previous studies conducted over the past 10 years that have called into question the verity of the tenets of, and therefore the value of the application of, the equivalent spherical volume diameter theory and the random particle orientation model to the interpretation of LD psa results from measurements made on nonspherical particles.

  11. Comparison of Result Times Between Urine and Whole Blood Point-of-care Pregnancy Testing. (United States)

    Gottlieb, Michael; Wnek, Kristopher; Moskoff, Jordan; Christian, Errick; Bailitz, John


    Point-of-care (POC) pregnancy testing is commonly performed in the emergency department (ED). One prior study demonstrated equivalent accuracy between urine and whole blood for one common brand of POC pregnancy testing. Our study sought to determine the difference in result times when comparing whole blood versus urine for the same brand of POC pregnancy testing. We conducted a prospective, observational study at an urban, academic, tertiary care hospital comparing the turnaround time between order and result for urine and whole blood pregnancy tests collected according to standard protocol without intervention from the investigators. After the blood was collected, the nurse would place three drops onto a Beckman Coulter ICON 25 Rapid HCG bedside pregnancy test and set a timer for 10 minutes. At the end of the 10 minutes, the result and time were recorded on an encoded data sheet and not used clinically. The same make and model analyzer was also used for urine tests in the lab located within the ED. The primary outcome was the difference in mean turnaround time between whole blood in the ED and urine testing in the adjacent lab results. Concordance between samples was assessed as a secondary outcome. 265 total patients were included in the study. The use of whole blood resulted in a mean time savings of 21 minutes (95% CI 16-25 minutes) when compared with urine (ptesting may reduce the total result turnaround time without significant changes in accuracy in this single-center study.

  12. Detection of ingested nitromethane and reliable creatinine assessment using multiple common analytical methods. (United States)

    Murphy, Christine M; Devlin, John J; Beuhler, Michael C; Cheifetz, Paul; Maynard, Susan; Schwartz, Michael D; Kacinko, Sherri


    Nitromethane, found in fuels used for short distance racing, model cars, and model airplanes, produces a falsely elevated serum creatinine with standard creatinine analysis via the Jaffé method. Erroneous creatinine elevation often triggers extensive testing, leads to inaccurate diagnoses, and delayed or inappropriate medical interventions. Multiple reports in the literature identify "enzymatic assays" as an alternative method to detect the true value of creatinine, but this ambiguity does not help providers translate what type of enzymatic assay testing can be done in real time to determine if there is indeed false elevation. We report seven cases of ingested nitromethane where creatinine was determined via Beckman Coulter ® analyser using the Jaffé method, Vitros ® analyser, or i-Stat ® point-of-care testing. Nitromethane was detected and semi-quantified using a common clinical toxic alcohol analysis method, and quantified by headspace-gas chromatography-mass spectrometry. When creatinine was determined using i-Stat ® point-of-care testing or a Vitros ® analyser, levels were within the normal range. Comparatively, all initial creatinine levels obtained via the Jaffé method were elevated. Nitromethane concentrations ranged from 42 to 310 μg/mL. These cases demonstrate reliable assessment of creatinine through other enzymatic methods using a Vitros ® analyser or i-STAT ® . Additionally, nitromethane is detectable and quantifiable using routine alcohols gas chromatography analysis and by headspace-gas chromatography-mass spectrometry.

  13. Journal of Computational Electronics: Proceedings of the International Workshop on Computational Electronics (8th) (IWCE-8), Beckman Institute, University of Illinois, 15-18 October 2001. Volume 1, Issue 1-2 (United States)


    Chris Bowen, 1997. J. Appl. Phys. 81: 3207. Dr. Roger Lake and Dr. Timothy B. Boykin. I would Cwik T., Klimeck G., McAuley M., Norton C., Sterling T...quan- tTo whom correspondence should be addressed, tum effects in the SOI MOSFET structure depicted in 268 Raney gate packet. As a result of this

  14. Journal of Computational Electronics: Proceedings of the International Workshop on Computational Electronics (8th) (IWCE-8), Beckman Institute, University of Illinois, 15-18 October 2001. Volume 1, Issue 1-2

    National Research Council Canada - National Science Library

    Ferry, David K


    .... The community typically attending IWCE best represents the audience addressed by the Journal of Computational Electronics, but the composition of this community has grown over the years to include...

  15. Reliable and accurate CD4+ T cell count and percent by the portable flow cytometer CyFlow MiniPOC and "CD4 Easy Count Kit-Dry", as revealed by the comparison with the gold standard dual platform technology.

    Directory of Open Access Journals (Sweden)

    Milena Nasi

    Full Text Available An accurate and affordable CD4+ T cells count is an essential tool in the fight against HIV/AIDS. Flow cytometry (FCM is the "gold standard" for counting such cells, but this technique is expensive and requires sophisticated equipment, temperature-sensitive monoclonal antibodies (mAbs and trained personnel. The lack of access to technical support and quality assurance programs thus limits the use of FCM in resource-constrained countries. We have tested the accuracy, the precision and the carry-over contamination of Partec CyFlow MiniPOC, a portable and economically affordable flow cytometer designed for CD4+ count and percentage, used along with the "CD4% Count Kit-Dry".Venous blood from 59 adult HIV+ patients (age: 25-58 years; 43 males and 16 females was collected and stained with the "MiniPOC CD4% Count Kit-Dry". CD4+ count and percentage were then determined in triplicate by the CyFlow MiniPOC. In parallel, CD4 count was performed using mAbs and a CyFlow Counter, or by a dual platform system (from Beckman Coulter based upon Cytomic FC500 ("Cytostat tetrachrome kit" for mAbs and Coulter HmX Hematology Analyzer (for absolute cell count.The accuracy of CyFlow MiniPOC against Cytomic FC500 showed a correlation coefficient (CC of 0.98 and 0.97 for CD4+ count and percentage, respectively. The accuracy of CyFlow MiniPOC against CyFlow Counter showed a CC of 0.99 and 0.99 for CD4 T cell count and percentage, respectively. CyFlow MiniPOC showed an excellent repeatability: CD4+ cell count and percentage were analyzed on two instruments, with an intra-assay precision below ± 5% deviation. Finally, there was no carry-over contamination for samples at all CD4 values, regardless of their position in the sequence of analysis.The cost-effective CyFlow MiniPOC produces rapid, reliable and accurate results that are fully comparable with those from highly expensive dual platform systems.


    Directory of Open Access Journals (Sweden)

    C. Berkan ALPAY


    Full Text Available The aim of this study is to determine the difference between body compositions and some mineral levels of elite wrestlers (at an international level who have loss of weight and who have not loss of weight. 69 volunteer elite wrestlers inv olving in Turkey Championship participated in this study. The wrestler who had loss of weight and who had not loss of weight in order to compete in their weight class before the championship are randomly chosen and at the time of race weighing, an informat ion form including demographic information, Tanita BC 418, bioelectrical impedance analyzer (BİA with USA brand, body compositions and 5 cc of blood from their forearm venous with vacutainer ( closed blood collection system are received from wrestlers who participated in the study. The blood samples are divided into serums by centrifuged at 4000 rpm in 5 minutes by using NF - 400 model centrifuge device with Nüve brand. Whether the wrestlers have loss of weight or not is calculated with a formula after their Sodium (Na + , Blood Urea Nitrogen (BUN and Glucose levels are analyzed and they are separated into two groups according to their Plasma Osmolarity (P Osm levels. The wrestlers whose P Osm level ≤290 are discussed as the group not having loss of weight and the wrestler whose P Osm level >290 are discussed as the group having loss of weight. The information about the amount of loss of weight that they have before a race as a demographic variable is received from the sportsmen participating in this study. The basal metabolic rate, percentage of body fat, fat mass, free fat mass, total body water was determined with BİA. In the blood samples; Na + , BUN, Glucose, Iron (Fe, Calcium (Ca, Potassium (K an d Chlorine (Cl levels are measured by using kits with brand of Beckman Coulter in the AU2700 Plus model biochemical auto analyzer with brand of Beckman Coulter and Vitamin B12 and insulin levels are measured by using kits with brand of Roche in the Cobas E

  17. A single-centre evaluation of two new anti-Mullerian hormone assays and comparison with the current clinical standard assay. (United States)

    Welsh, Paul; Smith, Karen; Nelson, Scott M


    Are the new Ansh Labs Ultra-Sensitive anti-Müllerian hormone (AMH) and picoAMH ELISA assays suitable for clinical use and is the Ultra-Sensitive assay comparable to the Beckman Coulter AMH Gen II assay? The Ultra-Sensitive assay appears to have different calibration to the Gen II assay, but has performance characteristics generally suitable for clinical use. The Gen II assay is the most commonly used AMH assay in routine biochemistry at present, but persistent calibration/interference problems have been reported. Serum from patients referred for AMH measurement was assayed (in duplicate) using the Gen II assay in Glasgow Royal Infirmary between January and February 2013. We randomly selected 193 stored serum samples to re-run (in duplicate) using the Ultra-Sensitive AMH Ansh Labs assay, blinded to the original result. Samples that returned low results were run on the picoAMH Ansh Labs assay. Performance characteristics and linearity of the new assays were also assessed. All serum samples from patients referred for AMH at Glasgow Royal Infirmary between January and February 2013 were eligible for inclusion. Investigators were blinded to any identifiable information regarding the patients, including sex, age and reason for AMH measurement. Intra-assay and inter-assay coefficients of variation of the Ultra-Sensitive and picoAMH assays were ≤6.0 and ≤10.7%, respectively, over a range of concentrations. The assays had mean linearity of 98 and 97% over the dilution range of 1:2-1:16 and 1:2-1:8, respectively. The limit of detection of the ultrasensitive assay was calculated to be 0.34 pmol/l. For 166 samples which provided a quantitative result on the Gen II and Ultra-Sensitive Ansh Labs assays, the median (interquartile range) was 12.2 (3.4-29.3) pmol/l and 20.0 (6.6-36.8) pmol/l, respectively (Py (Ultra-Sensitive) = 1.7 + 1.4 × Gen II. More samples were below the clinical cut-off of 5.4 pmol/l using the Gen II assay (a difference between paired proportions of 15

  18. A new database sub-system for grain-size analysis (United States)

    Suckow, Axel


    Detailed grain-size analyses of large depth profiles for palaeoclimate studies create large amounts of data. For instance (Novothny et al., 2011) presented a depth profile of grain-size analyses with 2 cm resolution and a total depth of more than 15 m, where each sample was measured with 5 repetitions on a Beckman Coulter LS13320 with 116 channels. This adds up to a total of more than four million numbers. Such amounts of data are not easily post-processed by spreadsheets or standard software; also MS Access databases would face serious performance problems. The poster describes a database sub-system dedicated to grain-size analyses. It expands the LabData database and laboratory management system published by Suckow and Dumke (2001). This compatibility with a very flexible database system provides ease to import the grain-size data, as well as the overall infrastructure of also storing geographic context and the ability to organize content like comprising several samples into one set or project. It also allows easy export and direct plot generation of final data in MS Excel. The sub-system allows automated import of raw data from the Beckman Coulter LS13320 Laser Diffraction Particle Size Analyzer. During post processing MS Excel is used as a data display, but no number crunching is implemented in Excel. Raw grain size spectra can be exported and controlled as Number- Surface- and Volume-fractions, while single spectra can be locked for further post-processing. From the spectra the usual statistical values (i.e. mean, median) can be computed as well as fractions larger than a grain size, smaller than a grain size, fractions between any two grain sizes or any ratio of such values. These deduced values can be easily exported into Excel for one or more depth profiles. However, such a reprocessing for large amounts of data also allows new display possibilities: normally depth profiles of grain-size data are displayed only with summarized parameters like the clay

  19. Rapid prediction of stem cell mobilization using volume and conductivity data from automated hematology analyzers. (United States)

    Villa, Carlos H; Porturas, Thomas; Sell, Mary; Wall, Mark; DeLeo, Gene; Fetters, Jenna; Mignono, Sam; Irwin, Leah; Hwang, Wei-Ting; O'Doherty, Una


    Rapid analytics to predict circulating hematopoietic stem cells are valuable for optimal management of mobilization, particularly for the use of newer and costly mobilization agents such as plerixafor. We used stepwise, linear multiple regression modeling applied to cell population data collected by routine hematology analyzers (Beckman Coulter DxH 800) on patients undergoing autologous stem cell collection (n = 131). Beta coefficients were used to derive a formula for a stem cell index (SCI). We then tested the correlation of SCI with stem cell counts and performance of the SCI as a predictor of poor mobilization with external validation in a separate cohort (n = 183). The SCI correlated strongly with CD34 counts by flow cytometry (r = 0.8372 in the development cohort, r = 0.8332 in the external validation cohort) and compares favorably with other rapid stem cell enumerating technologies. In the external validation cohort, the SCI performed well as a predictor (receiver operating characteristic area under the curve, 0.9336) of poor mobilization (CD34 count < 10), with a sensitivity of 72% and a specificity of 93%. When prevalence of poor mobilization was 33%, this resulted in a positive predictive value of 83% and a negative predictive value of 87%. The SCI also showed promise in tracking responses to plerixafor administration. The findings demonstrate the utility of the cell population data collected by hematology analyzers to provide rapid data beyond standard complete blood counts, particularly for stem cell count prediction, requiring no additional reagents, specimen, or instrumentation. © 2017 AABB.

  20. Cardiovascular risk predictors in central Slovakian Roma children and adolescents: regional differences. (United States)

    Hujová, Zuzana; Alberty, Roman; Ahlers, Ivan; Ahlersová, Eva; Paulíková, Edita; Desatniková, Júlia; Gábor, Drahoslav; Hrubá, Frantiska


    The objective of the study was to determine some Cardiovascular Disease (CVD) risk factors in 174 Roma children and adolescents (88 males and 86 females) aged 7-18 in 3 Central Slovakian cities (44 from Zilina, 39 from Bansk, Bystrica and 91 from Rimavská Sobota). Venous blood samples were drawn in the morning, after a 12 hour overnight fast for biochemical analysis. Total cholesterol (TC) and triglycerides (TG) were determined enzymatically. HDL-cholesterol (HDL-C) after selective precipitation lipoproteins containing apolipoprotein B and LDL-cholesterol (LDL-C) was calculated by the Friedewald Formula. Serum levels of apolipoproteins (apo A, apo B) were analyzed immunochemically. Concentration of lipoprotein a [Lp(a)] was analyzed by immunonephelometric method (Beckman-Coulter System). Anthropometric measurements, including weight, height, waist and hip circumference were used to calculate the sum of the body mass index (BMI) and waist to hip ratio (WHR). Measured blood pressure (BP) was used to classify for hypertension. Significant differences were determined in serum levels of LDL-C (p Zilina and Rimavská Sobota p Zilina's population (WHR significantly connected with family history CVD p = 0.03, cigarette smoking p = 0.02 and leisure time physical activity p < 0.001) and BMI, apo A and BP in Banská Bystrica. WHR was positively correlated to BP and negatively to HDL-C and TG only in Roma participants from Rimavská Sobota. BMI was positively correlated to systolic BP in populations from Banská Bystrica and Rimavská Sobota. The results of the study should improve the paediatric health treatment and prevention of CVD risk predictors for Roma from different cities.

  1. Detection of serum antimüllerian hormone in women approaching menopause using sensitive antimüllerian hormone enzyme-linked immunosorbent assays. (United States)

    Robertson, David M; Kumar, Ajay; Kalra, Bhanu; Shah, Shivani; Pruysers, Enid; Vanden Brink, Heidi; Chizen, Donna; Visser, Jenny A; Themmen, Axel P; Baerwald, Angela


    Current antimüllerian hormone (AMH) immunoassays are insufficiently sensitive to detect circulating AMH levels in ovulatory women approaching menopause. The aim of this study was to detect serum AMH levels across the menstrual cycle with age, using two new AMH enzyme-linked immunosorbent assay (ELISA) kits with increased sensitivity and differing specificity. Serum AMH levels were determined every 2 to 3 days across the interovulatory interval of menstrual cycles among women of early-mid reproductive age (18-35 y; n = 10) and late reproductive age (45-55 y; n = 17). Two highly sensitive AMH ELISAs (designated 24/32 and 24/37) with differing sensitivities were developed and applied to sera using a recombinant human pro-mature AMH preparation as reference. A third AMH ELISA (Gen II AMH ELISA kit; Beckman Coulter, Brea, CA) used was directed on mature-pro regions of AMH. AMH levels in all cycles were detectable with the 24/32 and 24/37 AMH ELISAs. AMH levels across the menstrual cycle were highly correlated (r = 0.98) between the 24/32 and 24/37 AMH ELISAs and the Gen II AMH ELISA (r = 0.94), but with large intracycle variations observed in older women. In late reproductive age, more than 95% of AMH values were detectable with the 24/32 and 24/37 AMH ELISAs, whereas only 36% of AMH values were detectable with the Gen II AMH ELISA. AMH levels were detected in cycles with lower antral follicle count and at a later age using the 24/32 and 24/37 AMH ELISAs compared with the Gen II AMH ELISA. AMH level correlated with antral follicle count in younger women, but not in older women. The new 24/32 and 24/37 AMH ELISAs have the sensitivity to monitor ovarian follicle profiles in late reproductive age.

  2. A European multicientre study on the comparison of HBV viral loads between VERIS HBV assay and Roche COBAS® TAQMAN® HBV test, Abbott RealTime HBV assay, Siemens VERSANT HBV assay, and Qiagen artus HBV RG kit. (United States)

    Braun, Patrick; Delgado, Rafael; Drago, Monica; Fanti, Diana; Fleury, Hervé; Izopet, Jacques; Lombardi, Alessandra; Marcos, MaAngeles; Sauné, Karine; O'Shea, Siobhan; Pérez-Rivilla, Alfredo; Ramble, John; Trimoulet, Pascale; Vila, Jordi; Whittaker, Duncan; Artus, Alain; Rhodes, Daniel


    Hepatitis B viral load testing is essential to treatment and monitoring decisions in patients with chronic Hepatitis B. Beckman Coulter has developed the VERIS HBV Assay (Veris) for use on the fully automated DxN VERIS Molecular Diagnostics System. 1 OBJECTIVES: To evaluate the clinical performance of the Veris HBV Assay at multiple EU laboratories STUDY DESIGN: Method comparison was performed with a total of 344 plasma specimens from HBV infected patients tested with Veris and COBAS ® TaqMan ® HBV Test (Cobas), 207 specimens tested with Veris and RealTime HBV Assay (RealTime), 86 specimens tested with Veris and VERSANT ® HBV Assay (Versant), and 74 specimens tested with Veris and artus ® HBV RG PCR kit (artus). Bland-Altman analysis showed average bias of -0.46 log 10 IU/mL between Veris and Cobas, -0.46 log 10 IU/mL between Veris and RealTime, -0.36 log 10 IU/mL between Veris and Versant, and -0.12 log 10 IU/mL between Veris and artus. Bias was consistent across the assay range. Patient monitoring results using Veris demonstrated similar viral load trends over time to Cobas, RealTime, and artus. The VERIS HBV Assay demonstrated comparable clinical performance, with varying degrees of negative bias, compared to other currently marketed assays for HBV DNA monitoring. This negative bias should be taken into consideration if switching monitoring methods to Veris. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Efavirenz interference in urine screening immunoassays for tetrahydrocannabinol. (United States)

    Oosthuizen, Nicholette M; Laurens, Johannes B


    It has been known for some time that the antiretroviral drug, efavirenz (EFV), cross-reacts in urine immunoassays for tetrahydrocannabinol (THC). Because published studies investigating this phenomenon are limited, cross-reactivity information for several immunoassays is lacking. Reports of possible false-positive THC results from clinicians conducting workplace testing prompted us to investigate cross-reactivity for assays frequently employed in our own setting. In light of the potentially deleterious consequences of misclassification, information about EFV cross-reactivity should be included in product information to facilitate interpretation of results and assay selection. Random urine samples from 30 patients on EFV therapy were analysed for THC metabolites by two near-testing devices (THC One Step Marijuana and Rapid Response(®) Drugs of Abuse Test Strips) and two automated immunoassays (Roche Diagnostics Cannabinoids II and Beckman Coulter SYNCHRON(®) Systems THC2). THC confirmatory testing was performed by gas chromatography-mass spectrometry (GC-MS). GC-MS failed to detect THC metabolites in any of the samples, as did three of the four immunoassays. However, the Rapid Response(®) test strips yielded positive results in 28 out of 30 samples, which could be reversed on re-testing after sample pretreatment with glucuronidase. Our study supports previous findings that interference is attributable to a glucuronidated EFV metabolite. We postulate that cross-reactivity is influenced by the composition of immunogens used to elicit anti-THC antibodies. Since access to such information is restricted, contributions from scientists in the antibody industry may be enlightening.

  4. Improved differential diagnosis of anemia of chronic disease and iron deficiency anemia: a prospective multicenter evaluation of soluble transferrin receptor and the sTfR/log ferritin index. (United States)

    Skikne, Barry S; Punnonen, Kari; Caldron, Paul H; Bennett, Michael T; Rehu, Mari; Gasior, Gail H; Chamberlin, Janna S; Sullivan, Linda A; Bray, Kurtis R; Southwick, Paula C


    Anemia of chronic disease (ACD) and iron deficiency anemia (IDA) are the most prevalent forms of anemia and often occur concurrently. Standard tests of iron status used in differential diagnosis are affected by inflammation, hindering clinical interpretation. In contrast, soluble transferrin receptor (sTfR) indicates iron deficiency and is unaffected by inflammation. Objectives of this prospective multicenter clinical trial were to evaluate and compare the diagnostic accuracy of sTfR and the sTfR/log ferritin index (sTfR Index) for differential diagnosis using the automated Access(®) sTfR assay (Beckman Coulter) and sTfR Index. We consecutively enrolled 145 anemic patients with common disorders associated with IDA and ACD. Subjects with IDA or ACD + IDA had significantly higher sTfR and sTfR Index values than subjects with ACD (P < 0.0001). ROC curves produced the following cutoffs for sTfR: 21 nmol/L (or 1.55 mg/L), and the sTfR Index: 14 (using nmol/L) (or 1.03 using mg/L). The sTfR Index was superior to sTfR (AUC 0.87 vs. 0.74, P < 0.0001). Use of all three parameters in combination more than doubled the detection of IDA, from 41% (ferritin alone) to 92% (ferritin, sTfR, sTfR Index). Use of sTfR and the sTfR Index improves detection of IDA, particularly in situations where routine markers provide equivocal results. Findings demonstrate a significant advantage in the simultaneous determination of ferritin, sTfR and sTfR Index. Obtaining a ferritin level alone may delay diagnosis of combined IDA and ACD. Copyright © 2011 Wiley-Liss, Inc.

  5. Anti-Müllerian hormone (AMH) levels in premenopausal breast cancer patients treated with taxane-based adjuvant chemotherapy - A translational research project of the SUCCESS A study. (United States)

    Trapp, Elisabeth; Steidl, J; Rack, B; Kupka, M S; Andergassen, U; Jückstock, J; Kurt, A; Vilsmaier, T; de Gregorio, A; de Gregorio, N; Tzschaschel, M; Lato, C; Polasik, A; Tesch, H; Schneeweiss, A; Beckmann, M W; Fasching, P A; Janni, W; Müller, V


    Premenopausal women undergoing chemotherapy are at high risk for premature ovarian failure and its long-term consequences. Data on potential markers to evaluate ovarian reserve pre- and posttreatment are limited. Anti-Müllerian hormone (AMH) known for ovarian reserve in reproductive medicine could be a surrogate marker and was assessed in premenopausal breast cancer patients of the SUCCESS A study (EUDRA-CT no. 2005-000490-21). We identified 170 premenopausal patients, age ≤ 40 years at trial entry, who received FEC-Doc as taxane-anthracylince based chemotherapy. Blood samples were taken at three time points: Before, four weeks after and two years after adjuvant chemotherapy. Serum AMH-levels were evaluated in a central laboratory by a quantitative immunoassay AMH Gen II ELISA (Beckman Coulter, Brea, USA). Median age was 36 years (21-40 years). Median serum AMH-level before chemotherapy was 1.37 ng/ml (range < 0.1-11.3 ng/ml). Four weeks after chemotherapy AMH-levels dropped in 98.6% of the patients to <0.1 ng/ml (range < 0.1-0.21 ng/ml). After two years, 73.3% (n = 101) showed no evidence of ovarian function recovery (AMH <0.1 ng/ml, range < 0.1-3.9 ng/ml). Permanent chemotherapy induced amenorrhea occurred only in 50.6% of the patients. In this analysis, premenopausal patients showed a high rate of ovarian impairment reflected by low AMH-levels after chemotherapy. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. Diurnal Variations of Circulating Extracellular Vesicles Measured by Nano Flow Cytometry.

    Directory of Open Access Journals (Sweden)

    Kirsty M Danielson

    Full Text Available The identification of extracellular vesicles (EVs as intercellular conveyors of biological information has recently emerged as a novel paradigm in signaling, leading to the exploitation of EVs and their contents as biomarkers of various diseases. However, whether there are diurnal variations in the size, number, and tissue of origin of blood EVs is currently not known, and could have significant implications when using EVs as biomarkers for disease progression. Currently available technologies for the measurement of EV size and number are either time consuming, require specialized equipment, or lack sufficient accuracy across a range of EV sizes. Flow cytometry represents an attractive alternative to these methods; however, traditional flow cytometers are only capable of measuring particles down to 500 nm, which is significantly larger than the average and median sizes of plasma EVs. Utilizing a Beckman Coulter MoFlo XDP flow cytometer with NanoView module, we employed nanoscale flow cytometry (termed nanoFCM to examine the relative number and scatter distribution of plasma EVs at three different time points during the day in 6 healthy adults. Analysis of liposomes and plasma EVs proved that nanoFCM is capable of detecting biologically-relevant vesicles down to 100 nm in size. With this high resolution configuration, we observed variations in the relative size (FSC/SSC distributions and concentration (proportions of EVs in healthy adult plasma across the course of a day, suggesting that there are diurnal variations in the number and size distribution of circulating EV populations. The use of nanoFCM provides a valuable tool for the study of EVs in both health and disease; however, additional refinement of nanoscale flow cytometric methods is needed for use of these instruments for quantitative particle counting and sizing. Furthermore, larger scale studies are necessary to more clearly define the diurnal variations in circulating EVs, and thus

  7. Key performance indicators score (KPIs-score) based on clinical and laboratorial parameters can establish benchmarks for internal quality control in an ART program. (United States)

    Franco, José G; Petersen, Claudia G; Mauri, Ana L; Vagnini, Laura D; Renzi, Adriana; Petersen, Bruna; Mattila, M C; Comar, Vanessa A; Ricci, Juliana; Dieamant, Felipe; Oliveira, João Batista A; Baruffi, Ricardo L R


    KPIs have been employed for internal quality control (IQC) in ART. However, clinical KPIs (C-KPIs) such as age, AMH and number of oocytes collected are never added to laboratory KPIs (L-KPIs), such as fertilization rate and morphological quality of the embryos for analysis, even though the final endpoint is the evaluation of clinical pregnancy rates. This paper analyzed if a KPIs-score strategy with clinical and laboratorial parameters could be used to establish benchmarks for IQC in ART cycles. In this prospective cohort study, 280 patients (36.4±4.3years) underwent ART. The total KPIs-score was obtained by the analysis of age, AMH (AMH Gen II ELISA/pre-mixing modified, Beckman Coulter Inc.), number of metaphase-II oocytes, fertilization rates and morphological quality of the embryonic lot. The total KPIs-score (C-KPIs+L-KPIs) was correlated with the presence or absence of clinical pregnancy. The relationship between the C-KPIs and L-KPIs scores was analyzed to establish quality standards, to increase the performance of clinical and laboratorial processes in ART. The logistic regression model (LRM), with respect to pregnancy and total KPIs-score (280 patients/102 clinical pregnancies), yielded an odds ratio of 1.24 (95%CI = 1.16-1.32). There was also a significant difference (pperformed to assess quality standards. This total KPIs-score could set up benchmarks for clinical pregnancy. Moreover, IQC can use C-KPIs and L-KPIs scores to detect problems in the clinical-laboratorial interface.

  8. Emergency medical technician-performed point-of-care blood analysis using the capillary blood obtained from skin puncture. (United States)

    Kim, Changsun; Kim, Hansol


    Comparing a point-of-care (POC) test using the capillary blood obtained from skin puncture with conventional laboratory tests. In this study, which was conducted at the emergency department of a tertiary care hospital in April-July 2017, 232 patients were enrolled, and three types of blood samples (capillary blood from skin puncture, arterial and venous blood from blood vessel puncture) were simultaneously collected. Each blood sample was analyzed using a POC analyzer (epoc® system, USA), an arterial blood gas analyzer (pHOx®Ultra, Nova biomedical, USA) and venous blood analyzers (AU5800, DxH2401, Beckman Coulter, USA). Twelve parameters were compared between the epoc and reference analyzers, with an equivalence test, Bland-Altman plot analysis and linear regression employed to show the agreement or correlation between the two methods. The pH, HCO 3 , Ca 2+ , Na + , K + , Cl - , glucose, Hb and Hct measured by the epoc were equivalent to the reference values (95% confidence interval of mean difference within the range of the agreement target) with clinically inconsequential mean differences and narrow limits of agreement. All of them, except pH, had clinically acceptable agreements between the two methods (results within target value ≥80%). Of the remaining three parameters (pCO 2, pO 2 and lactate), the epoc pCO 2 and lactate values were highly correlated with the reference device values, whereas pO 2 was not. (pCO 2 : R 2 =0.824, y=-1.411+0.877·x; lactate: R 2 =0.902, y=-0.544+0.966·x; pO 2 : R 2 =0.037, y=61.6+0.431·x). Most parameters, except only pO 2 , measured by the epoc were equivalent to or correlated with those from the reference method. Copyright © 2017 Elsevier Inc. All rights reserved.

  9. Microparticle sizing by dynamic light scattering in fresh-frozen plasma. (United States)

    Lawrie, A S; Albanyan, A; Cardigan, R A; Mackie, I J; Harrison, P


    We have previously shown that fresh-frozen plasma (FFP) contains red blood cell-derived procoagulant microparticles (MPs) that are removable by 0.2 microm filtration. Given the limitations of current methods for accurately sizing MPs, we have applied the novel approach of dynamic light scattering (DLS) to characterize the size distributions of these MPs within FFP. Fresh-frozen plasma was prepared from blood Group A and O donations (n = 10 of each) after an overnight hold of whole blood at 4 degrees C. On the day of analysis, plasma was thawed to 37 degrees C and daughter aliquots were studied pre- and post-filtration (0.2 microm filtration device, Ceveron MFU-500, Technoclone). MP size and dispersity was assessed using a Zetasizer Nano S (Malvern Instruments Ltd), which employs a 173 degrees backscatter detector and an N5 Submicron Particle Size Analyser (Beckman Coulter) using multi-angle measurements (30.1 degrees , 62.6 degrees and 90 degrees ). The analysers presented MP size distribution graphically as intensity plots, mean size, standard deviation and polydispersity index. Of the instruments used, only the N5 utilizing a 30.1 degrees angle of measurement could detect MPs of the expected size distribution and demonstrate their removal by filtration. MPs (range of mean particle diameters: pre, 101-464 nm; post, 21-182 nm filtration) were significantly smaller post-filtration (P size of MPs from blood Group O (pre, 247 nm) and Group A (pre, 289 nm) samples (P = 0.44). Our data demonstrates that DLS offers a novel approach to assessing MP size and distribution, a technique that could be easily adopted as a means of assessing MPs within either FFP or other blood products.

  10. A nationwide multicentre study in Turkey for establishing reference intervals of haematological parameters with novel use of a panel of whole blood (United States)

    Ozarda, Yesim; Ichihara, Kiyoshi; Bakan, Ebubekir; Polat, Harun; Ozturk, Nurinnisa; Baygutalp, Nurcan K.; Taneli, Fatma; Guvenc, Yesim; Ormen, Murat; Erbayraktar, Zubeyde; Aksoy, Nurten; Sezen, Hatice; Demir, Meltem; Eskandari, Gulcin; Polat, Gurbuz; Mete, Nuriye; Yuksel, Hatice; Vatansev, Husamettin; Gun, Fatma; Akin, Okhan; Ceylan, Ozlem; Noyan, Tevfik; Gozlukaya, Ozgul; Aliyazicioglu, Yuksel; Kahraman, Sevim; Dirican, Melahat; Tuncer, Gul Ozlem; Kimura, Shogo; Eker, Pinar


    Introduction A nationwide multicentre study was conducted to establish well-defined reference intervals (RIs) of haematological parameters for the Turkish population in consideration of sources of variation in reference values (RVs). Materials and methods K2-EDTA whole blood samples (total of 3363) were collected from 12 laboratories. Sera were also collected for measurements of iron, UIBC, TIBC, and ferritin for use in the latent abnormal values exclusion (LAVE) method. The blood samples were analysed within 2 hours in each laboratory using Cell Dyn and Ruby (Abbott), LH780 (Beckman Coulter), or XT-2000i (Sysmex). A panel of freshly prepared blood from 40 healthy volunteers was measured in common to assess any analyser-dependent bias in the measurements. The SD ratio (SDR) based on ANOVA was used to judge the need for partitioning RVs. RIs were computed by the parametric method with/without applying the LAVE method. Results Analyser-dependent bias was found for basophils (Bas), MCHC, RDW and MPV from the panel test results and thus those RIs were derived for each manufacturer. RIs were determined from all volunteers’ results for WBC, neutrophils, lymphocytes, monocytes, eosinophils, MCV, MCH and platelets. Gender-specific RIs were required for RBC, haemoglobin, haematocrit, iron, UIBC and ferritin. Region-specific RIs were required for RBC, haemoglobin, haematocrit, UIBC, and TIBC. Conclusions With the novel use of a freshly prepared blood panel, manufacturer-specific RIs’ were derived for Bas, Bas%, MCHC, RDW and MPV. Regional differences in RIs were observed among the 7 regions of Turkey, which may be attributed to nutritional or environmental factors, including altitude. PMID:28694726

  11. Analytical Bias Exceeding Desirable Quality Goal in 4 out of 5 Common Immunoassays: Results of a Native Single Serum Sample External Quality Assessment Program for Cobalamin, Folate, Ferritin, Thyroid-Stimulating Hormone, and Free T4 Analyses. (United States)

    Kristensen, Gunn B B; Rustad, Pål; Berg, Jens P; Aakre, Kristin M


    We undertook this study to evaluate method differences for 5 components analyzed by immunoassays, to explore whether the use of method-dependent reference intervals may compensate for method differences, and to investigate commutability of external quality assessment (EQA) materials. Twenty fresh native single serum samples, a fresh native serum pool, Nordic Federation of Clinical Chemistry Reference Serum X (serum X) (serum pool), and 2 EQA materials were sent to 38 laboratories for measurement of cobalamin, folate, ferritin, free T4, and thyroid-stimulating hormone (TSH) by 5 different measurement procedures [Roche Cobas (n = 15), Roche Modular (n = 4), Abbott Architect (n = 8), Beckman Coulter Unicel (n = 2), and Siemens ADVIA Centaur (n = 9)]. The target value for each component was calculated based on the mean of method means or measured by a reference measurement procedure (free T4). Quality specifications were based on biological variation. Local reference intervals were reported from all laboratories. Method differences that exceeded acceptable bias were found for all components except folate. Free T4 differences from the uncommonly used reference measurement procedure were large. Reference intervals differed between measurement procedures but also within 1 measurement procedure. The serum X material was commutable for all components and measurement procedures, whereas the EQA materials were noncommutable in 13 of 50 occasions (5 components, 5 methods, 2 EQA materials). The bias between the measurement procedures was unacceptably large in 4/5 tested components. Traceability to reference materials as claimed by the manufacturers did not lead to acceptable harmonization. Adjustment of reference intervals in accordance with method differences and use of commutable EQA samples are not implemented commonly. © 2016 American Association for Clinical Chemistry.

  12. Circulating endothelial microparticles in female migraineurs with aura. (United States)

    Liman, Thomas G; Bachelier-Walenta, Katrin; Neeb, Lars; Rosinski, Jana; Reuter, Uwe; Böhm, Michael; Endres, Matthias


    Endothelial microparticles (EMPs) are vesicles that are released from activated endothelial cells and serve as a surrogate for endothelial dysfunction (ED). ED may be involved in migraine pathophysiology and contribute to the increased risk of ischemic stroke, particularly in female migraineurs with aura (MA). We sought to determine whether EMPs are elevated in women with MA. In this case-control study, EMPs were detected by analysing surface markers using fluorescence-activated cell sorting (FACS). Surface markers were measured covering the main cell lines relevant in cardiovascular disease like endothelial cells, platelets, monocytes and leucocytes. Microparticles (MPs) were identified in correlation to calibration by 1 -µm calibrator beads (Beckman Coulter). Arterial stiffness was assessed using fingertip tonometry and the heart rate-adjusted augmentation index (AI). We included 29 patients with MA and 29 matched controls. MA patients had significantly higher EMPs (CD62E(+)AnnexinV(+): 5142/µl vs 1535/µl; p monocytic (CD14(+)AnnexinV(+) 6378 vs 3161; p < 0.001), and platelet MPs (CD62P(+)CD42b(+)AnnexinV(+) 5450 vs 3204; p < 0.001). Activated EMPs (CD62E(+)AnnexinV(+)) correlated with heart-rate adjusted AI (r = 0.46; p < 001). EMP levels are significantly elevated in women with MA and correlated with increased AI. Our findings suggest that endothelial activation is present in women with MA. This might contribute to higher stroke risk in MA. © International Headache Society 2014 Reprints and permissions:

  13. Evaluation of the red cell hemolysis in packed red cells during processing and storage

    Directory of Open Access Journals (Sweden)

    Makroo R


    Full Text Available Introduction : Storage of red cells causes a progressive increase in hemolysis. In spite of the use of additive solutions for storage and filters for leucoreduction, some amount of hemolysis is still inevitable. The extent of hemolysis, however, should not exceed the permissible threshold for hemolysis even on the 42 nd day of storage. Study Design and Methods: Eighty units of packed red cells, 40 stored in SAGM post leucoreduction and 40 in ADSOL without leucoreduction filters, were evaluated for plasma hemoglobin by HemoCue Plasma Hemoglobin analyzer on the day of collection and on the 7 th , 14 th , 21 st , 28 th , 35 th and 42 nd days thereafter. The hemoglobin and hematocrit were also noted for all these units by the Beckman and Coulter analyzer. Percentage hemolysis was then calculated. Observations: Hemolysis progressively increased with the storage period in all the stored red cell units (SAGM as well ADSOL. However, on day 42 nd of storage, free hemoglobin in all the red cell units was within the permissible level (which is 0.8% according to the Council of Europe guidelines and 1% as per the US FDA guidelines. The mean percentage hemolysis was slightly higher in the SAGM-containing bags with an integral leucoreduction filter as compared to the bags containing ADSOL. However this difference was marginal and not statistically significant. Conclusion: Hemolysis of the red cells increases with storage. However, maximum hemolysis does not exceed the permissible limits at any time thereby indicating the effect of optimum processing and storage conditions on red cell hemolysis.

  14. Iterative Outlier Removal: A Method for Identifying Outliers in Laboratory Recalibration Studies. (United States)

    Parrinello, Christina M; Grams, Morgan E; Sang, Yingying; Couper, David; Wruck, Lisa M; Li, Danni; Eckfeldt, John H; Selvin, Elizabeth; Coresh, Josef


    Extreme values that arise for any reason, including those through nonlaboratory measurement procedure-related processes (inadequate mixing, evaporation, mislabeling), lead to outliers and inflate errors in recalibration studies. We present an approach termed iterative outlier removal (IOR) for identifying such outliers. We previously identified substantial laboratory drift in uric acid measurements in the Atherosclerosis Risk in Communities (ARIC) Study over time. Serum uric acid was originally measured in 1990-1992 on a Coulter DACOS instrument using an uricase-based measurement procedure. To recalibrate previous measured concentrations to a newer enzymatic colorimetric measurement procedure, uric acid was remeasured in 200 participants from stored plasma in 2011-2013 on a Beckman Olympus 480 autoanalyzer. To conduct IOR, we excluded data points >3 SDs from the mean difference. We continued this process using the resulting data until no outliers remained. IOR detected more outliers and yielded greater precision in simulation. The original mean difference (SD) in uric acid was 1.25 (0.62) mg/dL. After 4 iterations, 9 outliers were excluded, and the mean difference (SD) was 1.23 (0.45) mg/dL. Conducting only one round of outlier removal (standard approach) would have excluded 4 outliers [mean difference (SD) = 1.22 (0.51) mg/dL]. Applying the recalibration (derived from Deming regression) from each approach to the original measurements, the prevalence of hyperuricemia (>7 mg/dL) was 28.5% before IOR and 8.5% after IOR. IOR is a useful method for removal of extreme outliers irrelevant to recalibrating laboratory measurements, and identifies more extraneous outliers than the standard approach. © 2016 American Association for Clinical Chemistry.

  15. A ten-color tube with dried antibody reagents for the screening of hematological malignancies. (United States)

    Correia, R P; Rajab, A; Bento, L C; Alexandre, A M; Vaz, A C; Schimidell, D; Pedro, E C; Perin, F S; Nozawa, S T; Barroso, R S; Bacal, N S


    The workflow in clinical flow cytometry laboratories must constantly be reviewed to develop technical procedures that improve quality and productivity and reduce costs. Using the Beckman Coulter dry coating technology, we customized a ten-color tube with dried antibody reagents, designated the Duraclone screening tube (DST), for screening hematological malignancies. Here, we compared the applicability, clinical and numerical equivalence, and cost and time required for the technical procedures between the liquid reagents and the DST. The DST contains CD4 + Kappa-FITC, CD8 + Lambda-PE, CD3 + CD14-ECD, CD33-PE-Cy5.5, CD20 + CD56-PE-Cy7, CD34-APC, CD19-APC-AlexaFluor700, CD10-APC-AlexaFluor750, CD5-Pacific Blue, and CD45-Krome Orange. We evaluated 20 bone marrow samples, 13 peripheral blood samples, 6 lymph node biopsy samples, 5 fine-needle aspirate samples, 5 cerebrospinal fluid samples, and 1 pleural fluid sample. The DST was useful for more than 60% of our samples. It was able to enumerate the majority of the populations in all types of samples with a statistically acceptable correlation with the liquid reagents. The use of the DST translated into significant time and cost savings of 15.8% and 12.3%, respectively, compared with the use of the liquid reagent. The cost was reduced by $14.36 per sample. The DST is an efficient solution for screening hematological malignancies with improved quality, productivity, standardization, and sustainability. These improvements could benefit patients by providing faster diagnoses using a higher quality and lower cost reagent. © 2017 John Wiley & Sons Ltd.

  16. A European multicentre study on the comparison of HCV viral loads between VERIS HCV assay and COBAS® TaqMan® HCV Test and RealTime HCV Assay. (United States)

    Braun, Patrick; Drago, Monica; Fanti, Diana; Fleury, Hervé; Hofmann, Jörg; Izopet, Jacques; Kühn, Sebastian; Lombardi, Alessandra; Micheli, Valeria; Sauné, Karine; Trimoulet, Pascale; Whittaker, Duncan; Artus, Alain; Rhodes, Daniel


    Beckman Coulter has developed the VERIS HCV Assay for use on the new fully automated DxN VERIS Molecular Diagnostic System ¥ for HCV viral load monitoring. Evaluate the clinical performance of the new quantitative VERIS HCV Assay. Comparison was performed on 279 plasma specimens from HCV infected patients tested with the VERIS HCV Assay and COBAS ® Ampliprep/COBAS ® Taqman ® HCV Test and 369 specimens tested with the VERIS HCV Assay and RealTime HCV Assay. Patient monitoring sample results from four time points were also compared. The average bias between the VERIS HCV Assay and the COBAS ® Ampliprep/COBAS ® Taqman ® HCV Test was 0.04 log 10 IU/mL, while between the VERIS HCV Assay and the RealTime HCV Assay average bias was 0.21 log 10 IU/mL. Bias, however, was not consistent across the measuring range. Analysis at the lower end of quantification levels 50, 100, and 1000IU/mL showed a predicted bias for VERIS HCV Assay versus COBAS ® Ampliprep/COBAS ® Taqman ® HCV Test between -0.42 and -0.22 log 10 IU/mL and for VERIS HCV Assay versus RealTime HCV Assay between 0.00 and 0.13 log 10 IU/mL. Patient monitoring of HCV viral load over time demonstrated similar levels between VERIS HCV Assay results and COBAS ® Ampliprep/COBAS ® Taqman ® HCV Test (52 samples from 13 patients) and RealTime HCV Assay (112 samples from 28 patients). VERIS HCV Assay for use on the DxN VERIS Molecular Diagnostic System represents a reliable new tool for easy sample to result HCV RNA viral load monitoring. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. European Multicenter Study on Analytical Performance of Veris HIV-1 Assay. (United States)

    Braun, Patrick; Delgado, Rafael; Drago, Monica; Fanti, Diana; Fleury, Hervé; Hofmann, Jörg; Izopet, Jacques; Kalus, Ulrich; Lombardi, Alessandra; Marcos, Maria Angeles; Mileto, Davide; Sauné, Karine; O'Shea, Siobhan; Pérez-Rivilla, Alfredo; Ramble, John; Trimoulet, Pascale; Vila, Jordi; Whittaker, Duncan; Artus, Alain; Rhodes, Daniel W


    The analytical performance of the Veris HIV-1 assay for use on the new, fully automated Beckman Coulter DxN Veris molecular diagnostics system was evaluated at 10 European virology laboratories. The precision, analytical sensitivity, performance with negative samples, linearity, and performance with HIV-1 groups/subtypes were evaluated. The precision for the 1-ml assay showed a standard deviation (SD) of 0.14 log 10 copies/ml or less and a coefficient of variation (CV) of ≤6.1% for each level tested. The 0.175-ml assay showed an SD of 0.17 log 10 copies/ml or less and a CV of ≤5.2% for each level tested. The analytical sensitivities determined by probit analysis were 19.3 copies/ml for the 1-ml assay and 126 copies/ml for the 0.175-ml assay. The performance with 1,357 negative samples demonstrated 99.2% with not detected results. Linearity using patient samples was shown from 1.54 to 6.93 log 10 copies/ml. The assay performed well, detecting and showing linearity with all HIV-1 genotypes tested. The Veris HIV-1 assay demonstrated analytical performance comparable to that of currently marketed HIV-1 assays. (DxN Veris products are Conformité Européenne [CE]-marked in vitro diagnostic products. The DxN Veris product line has not been submitted to the U.S. FDA and is not available in the U.S. market. The DxN Veris molecular diagnostics system is also known as the Veris MDx molecular diagnostics system and the Veris MDx system.). Copyright © 2017 American Society for Microbiology.

  18. Clinical impact of prostate specific antigen (PSA) inter-assay variability on management of prostate cancer. (United States)

    Murthy, Vedang; Rishi, Anupam; Gupta, Sanjeev; Kannan, Sadhana; Mahantshetty, Umesh; Tongaonkar, Hemant; Bakshi, Ganesh; Prabhash, Kumar; Bhanushali, Paresh; Shinde, Bhoopal; Inamdar, Nitin; Shrivastava, Shyamkishore


    To evaluate the inter-assay variability of six commercially available prostate specific antigen (PSA) assays, its clinical impact in prostate cancer (PCa) and comparison of automated versus manual assays. Sera from 495 patients (425 with PCa and 70 men with Benign Prostatic Hyperplasia (BPH), were measured with six different assays [three automated assays (a-PSA) and three manual ELISA based assay (m-PSA)]. Variability, agreement and bias were measured and compared among assays using Bland Altman plots and Passing and Bablok regression analysis. The possible impact of inter-assay variability on important clinical scenarios was also studied. All the assays were well correlated (r: 0.88-0.98); however there was significant disagreement and bias between the systems, which were more pronounced among the a-PSA assays. The Bland Altman plot showed that the variability was high between the m-PSA assays and the standard Abbott system with mean difference of 3.8-5.8ng/ml. In contrast, the a-PSA had better agreement with mean difference of 0.8-2.3ng/ml. Beckman Coulter showed the best agreement to the institutional reference (slope-1.097; 95% CI: 1.06-1.14; p<0.05, and intercept-0.20; 95% CI-0.38-0.58; p<0.05, Passing Bablok). It led to significant variability in PCa risk stratification and failure to detect biochemical failure in more than 50% cases. The discrepancies between the assays lead to significant clinical misinterpretation with risk group migration and detection of biochemical failure post radiotherapy. There are significant discordances between automated and ELISA based assays. Copyright © 2015 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  19. Efficient clinical-scale enrichment of lymphocytes for use in adoptive immunotherapy using a modified counterflow centrifugal elutriation program. (United States)

    Powell, Daniel J; Brennan, Andrea L; Zheng, Zhaohui; Huynh, Hong; Cotte, Julio; Levine, Bruce L


    Clinical-scale lymphocyte enrichment from a leukapheresis product has been performed most routinely using costly magnetic bead separation systems that deplete monocytes, but this procedure may leave behind residual beads or antibodies in the enriched cell product. Counterflow centrifugal elutriation has been demonstrated previously to enrich monocytes efficiently for generation of dendritic cells. This study describes a modified elutriation procedure for efficient bead-free economical enrichment of lymphocytes from leukapheresis products from healthy donors and study subjects with human immunodeficiency virus (HIV) infection or malignancy. Modified program settings and conditions for the CaridianBCT Elutra device were investigated to optimize lymphocyte enrichment and recovery. Lymphocyte enrichment was measured using a novel approach utilizing cell sizing analysis on a Beckman Coulter Multisizer and confirmed by flow cytometry phenotypic analysis. Efficient enrichment and recovery of lymphocytes from leukapheresis cell products was achieved using modified elutriation settings for flow rate and fraction volume. Elutriation allowed for enrichment of larger numbers of lymphocytes compared with depletion of monocytes by bead adherence, with a trend toward increased lymphocyte purity and yield via elutriation, resulting in a substantial reduction in the cost of enrichment per cell. Importantly, significant lymphocyte enrichment could be accomplished using leukapheresis samples from healthy donors (n=12) or from study subjects with HIV infection (n=15) or malignancy (n=12). Clinical-scale closed-system elutriation can be performed efficiently for the selective enrichment of lymphocytes for immunotherapy protocols. This represents an improvement in cost, yield and purity over current methods that require the addition of monocyte-depleting beads.

  20. Diurnal Variations of Circulating Extracellular Vesicles Measured by Nano Flow Cytometry. (United States)

    Danielson, Kirsty M; Estanislau, Jessica; Tigges, John; Toxavidis, Vasilis; Camacho, Virginia; Felton, Edward J; Khoory, Joseph; Kreimer, Simion; Ivanov, Alexander R; Mantel, Pierre-Yves; Jones, Jennifer; Akuthota, Praveen; Das, Saumya; Ghiran, Ionita


    The identification of extracellular vesicles (EVs) as intercellular conveyors of biological information has recently emerged as a novel paradigm in signaling, leading to the exploitation of EVs and their contents as biomarkers of various diseases. However, whether there are diurnal variations in the size, number, and tissue of origin of blood EVs is currently not known, and could have significant implications when using EVs as biomarkers for disease progression. Currently available technologies for the measurement of EV size and number are either time consuming, require specialized equipment, or lack sufficient accuracy across a range of EV sizes. Flow cytometry represents an attractive alternative to these methods; however, traditional flow cytometers are only capable of measuring particles down to 500 nm, which is significantly larger than the average and median sizes of plasma EVs. Utilizing a Beckman Coulter MoFlo XDP flow cytometer with NanoView module, we employed nanoscale flow cytometry (termed nanoFCM) to examine the relative number and scatter distribution of plasma EVs at three different time points during the day in 6 healthy adults. Analysis of liposomes and plasma EVs proved that nanoFCM is capable of detecting biologically-relevant vesicles down to 100 nm in size. With this high resolution configuration, we observed variations in the relative size (FSC/SSC distributions) and concentration (proportions) of EVs in healthy adult plasma across the course of a day, suggesting that there are diurnal variations in the number and size distribution of circulating EV populations. The use of nanoFCM provides a valuable tool for the study of EVs in both health and disease; however, additional refinement of nanoscale flow cytometric methods is needed for use of these instruments for quantitative particle counting and sizing. Furthermore, larger scale studies are necessary to more clearly define the diurnal variations in circulating EVs, and thus further inform

  1. Detection of an IncA/C plasmid encoding VIM-4 and CMY-4 β-lactamases in Klebsiella oxytoca and Citrobacter koseri from an inpatient in a cardiac rehabilitation unit. (United States)

    Caltagirone, Mariasofia; Bitar, Ibrahim; Piazza, Aurora; Spalla, Melissa; Nucleo, Elisabetta; Navarra, Antonella; Migliavacca, Roberta


    A 62-year-old patient was transferred to the cardiac rehabilitation unit of the I.R.C.C.S. Fondazione S. Maugeri after undergoing a heart transplantation at the Acute Care Hospital I.R.C.C.S. S. Matteo of Pavia. On 1 August 2013 and during hospitalization in the rehabilitation unit, Klebsiella oxytoca and Citrobacter koseri clinical isolates were simultaneously recovered from the patient's preputial swab. Both the K. oxytoca and C. koseri strains were carbapenem- resistant by MicroScan System (Beckman Coulter). Carbapenem-resistant K. pneumoniae had previously been reported in the same rehabilitation facility. The aim of the study was to identify the carbapenem resistance mechanisms among the enterobacterial species recovered. Phenotypic screening tests useful to detect the β-lactamases/carbapenemases were performed. Carbapenem MICs were obtained by Etest. AmpC and MBL encoding genes were identified by PCR and sequencing. Conjugation assays and plasmid characterization were performed. Both of the K. oxytoca and C. koseri isolates were multi drug resistant, showing resistance to amoxicillin-clavulanic acid, three generation cephalosporins, ertapenem (K. oxytoca MIC, >32 mg/L; C. koseri MIC, 4 mg/L), imipenem (K. oxytoca MIC, 4 mg/L; C. koseri MIC, 12 mg/L), thrimethoprim sulphamethoxazole and gentamicin. Susceptibility was retained to fluoroquinolones, colistin and tigecycline. Molecular characterization confirmed the co-presence of blaCMY-4 and blaVIM-4 determinants in a 150 Kb transferable plasmid of IncA/C group. This case is the first detection in Italy of the K. oxytoca and C. koseri clinical isolates co-producing the CMY-4 and VIM-4 enzymes.

  2. Multilaboratory validation study of standardized multiple-locus variable-number tandem repeat analysis protocol for shiga toxin-producing Escherichia coli O157: a novel approach to normalize fragment size data between capillary electrophoresis platforms. (United States)

    Hyytia-Trees, Eija; Lafon, Patricia; Vauterin, Paul; Ribot, Efrain M


    The PulseNet USA subtyping network recently established a standardized protocol for multiple-locus variable-number tandem repeat analysis (MLVA) to characterize Shiga toxin-producing Escherichia coli O157. To enable data comparisons from different laboratories in the same database, reproducibility and high quality of the data must be ensured. The aim of this study was to test the robustness and reproducibility of the proposed standardized protocol by subjecting it to a multilaboratory validation process and to address any discrepancies that may have arisen from the study. A set of 50 strains was tested in 10 PulseNet participating laboratories that used capillary electrophoresis instruments from two manufacturers. Six out of the 10 laboratories were able to generate correct MLVA types for 46 (92%) or more strains. The discrepancies in MLVA type assignment were caused mainly by difficulties in optimizing polymerase chain reactions that were attributed to technical inexperience of the staff and suboptimal quality of reagents and instrumentation. It was concluded that proper training of staff must be an integral part of technology transfer. The interlaboratory reproducibility of fragment sizing was excellent when the same capillary electrophoresis platform was used. However, sizing discrepancies of up to six base pairs for the same fragment were detected between the two platforms. These discrepancies were attributed to different dye and polymer chemistries employed by the manufacturers. A novel software script was developed to assign alleles based on two platform-specific (Beckman Coulter CEQ8000 and Applied Biosystems Genetic Analyzer 3130xl) look-up tables containing fragment size ranges for all alleles. The new allele assignment method was validated at the PulseNet central laboratory using a diverse set of 502 Shiga toxin-producing Escherichia coli O157 isolates. The validation confirmed that the script reliably assigned the same allele for the same fragment

  3. The Effects of Short-Term Intensive Exercise on Levels of Liver Enzymes and Serum Lipids in Kick Boxing Athletes

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    Ömer Kaynar


    Full Text Available Objective: In this study, it was aimed to evaluate the ef­fects of short-term intensive exercise on liver enzymes and serum lipid levels with kick boxing athletes. Methods: 23 voluntary athletes who were between the ages of 15-46 and who engaged in kick–boxing have tak­en place this study. Athletes were made to do 45 minutes of warming-up, breathing, and stretching and 50 minutes of technical and tactical practices and then they were made to do a training match, which is equal to a 2 min­utes 3 circuits (1 minute rest kick-box match. In venous blood samples which were taken from athletes before and after training, aspartate aminotransferase (AST, alanine aminotransferase (ALT, alkaline phosphatase (ALP and gamma glutamine transpeptidase (GGT, enzyme activity and total cholesterol, high-density lipoprotein-cholesterol (HDL-C, low density lipoprotein-cholesterol (LDL-C and triglycerides serum levels were analyzed via spectropho­tometric method in Beckman Coulter AU 5800 auto ana­lyzer. Body composition measurements of athletes were made with Tanita TBF 300 brand device, which works with bio-impedance analysis (BIA system. Results: As a result of our study, statistically increases in serum ALT, AST, ALP and GGT enzyme activities and in serum total cholesterol, HDL-C and LDL-C levels were detected following short-term intensive exercise, but no significant difference was observed in TG levels after in­tensive exercise. Conclusion: The blows to the abdomen during kickbox­ing sports competitions result in increased liver enzymes and increased serum lipids may occur to meet energy de­mand of the body during exercise.

  4. Evaluation of a lateral flow-based technology card for blood typing using a simplified protocol in a model of extreme blood sampling conditions. (United States)

    Clavier, Benoît; Pouget, Thomas; Sailliol, Anne


    Life-threatening situations requiring blood transfusion under extreme conditions or in remote and austere locations, such as the battlefield or in traffic accidents, would benefit from reliable blood typing practices that are easily understood by a nonscientist or nonlaboratory technician and provide quick results. A simplified protocol was developed for the lateral flow-based device MDmulticard ABO-D-Rh subgroups-K. Its performance was compared to a reference method (PK7300, Beckman Coulter) in native blood samples from donors. The method was tested on blood samples stressed in vitro as a model of hemorrhage cases (through hemodilution using physiologic serum) and dehydration (through hemoconcentration by removing an aliquot of plasma after centrifugation), respectively. A total of 146 tests were performed on 52 samples; 126 in the hemodilution group (42 for each native, diluted 1/2, and diluted 1/4 samples) and 20 in the hemoconcentration group (10 for each native and 10% concentrated samples). Hematocrit in the tested samples ranged from 9.8% to 57.6% while hemoglobin levels ranged from 3.2 to 20.1 g/dL. The phenotype profile detected with the MDmulticard using the simplified protocol resulted in 22 A, seven B, 20 O, and three AB, of which nine were D- and five were Kell positive. No discrepancies were found with respect to the results obtained with the reference method. The simplified protocol for MDmulticard use could be considered a reliable method for blood typing in extreme environment or emergency situations, worsened by red blood cell dilution or concentration. © 2017 AABB.


    Directory of Open Access Journals (Sweden)

    Yu. A. Prokhorova


    Full Text Available Aim. Hereditary spherocytosis (HS is the most commonly encountered erythrocyte membranopathy. Frequency of occurrence of the disease makes one case per 2000−5000 newborns. Hereditary spherocytosis often causes a complex of clinical signs, including hemolytic crises in patients. At the same time many patients have asymptomatic HS. Differential diagnosis of HS is quite complex and in modern workload conditions the clinical doctors need a simpler diagnostics procedure. Patients and methods. Participants included 13 adults with verified hereditary spheroсytosis and 42 children with identified hereditary spherocytosis, 311 adults without hematological disorders, 42 children without hematological disorders. Verification of hereditary spherocytosis diagnosis was carried out using flow cytometry test (eosine-5 maleimid-binding, Deich`s method of determination of erythrocyte osmotic resistance and Sodium Dodecyl Sulfate-Poly Acrylamide Gel Electrophoresis. In this study we have assessed diagnostic value of hematological parameters provided by the hematological analyzer Beckman Coulter Cellular Analysis System DxH800 for identifying the degree of erythropoiesis disorder in patients with hereditary spherocytosis at the stage of reticulocytes maturation. According to our data, the ratio RET/IRF and calculated parameter MCV-MSCV can be used as the screening tests for hereditary spherocytosis. Results. Evaluation of the erythrocytes and reticulocytes parameters at the hematological analyzer identified the significant difference in estimate index MCV-MSCV (p < 0,0001, sensitivity 100%, specificity 100%, area under the ROC-curve 1,0 and RET/IRF (p < 0,0001, sensitivity 96,3%, specificity 94,1, area under the ROC-curve 0,97 between group of patients with HS and control group. We also evaluated the usability of eosine-5 maleimide binding in flow cytometry for verification of this membranopathy. For unify the test results we offer to use estimate indicator S

  6. Bone marrow mononuclears from murine tibia after spaceflight on biosatellite (United States)

    Andreeva, Elena; Roe, Maria; Buravkova, Ludmila; Andrianova, Irina; Goncharova, Elena; Gornostaeva, Alexandra

    Elucidation of the space flight effects on the adult stem and progenitor cells is an important goal in space biology and medicine. A unique opportunity for this is provided by project "BION -M1". The purpose of this study was to evaluate the effects of a 30-day flight on biosatellite "BION - M1" and the subsequent 7-day recovery on the quantity, viability, immunophenotype of mononuclears from murine tibia bone marrow. Also the in vitro characterization of functional capacity of multipotent mesenchymal stromal cells (MSCs) was scheduled. Under the project, the S57black/6 mice were divided into groups: spaceflight/vivarium control, recovery after spaceflight/ vivarium control to recovery. Bone marrow mononuclears were isolated from the tibia and immunophenotyped using antibodies against CD45, CD34, CD90 on a flow cytometer Epics XL (Beckman Coulter). A part of the each pool was frozen for subsequent estimation of hematopoietic colony-forming units (CFU), the rest was used for the evaluation of fibroblast CFU (CFUf) number, MSC proliferative activity and osteogenic potency. The cell number in the flight group was significantly lower than in the vivarium control group. There were no differences in this parameter between flight and control groups after 7 days of recovery. The mononuclears viability was more than 95 percent in all examined groups. Flow cytometric analysis showed no differences in the bone marrow cell immunophenotype (CD45, CD34, CD90.1 (Thy1)), but the flight animals had more large-sized CD45+mononuclears, than the control groups of mice. There was no difference in the CFUf number between groups. After 7 days in vitro the MSC number in flight group was twice higher than in vivarium group, after 10 days - 4 times higher. These data may indicate a higher proliferative activity of MSCs after spaceflight. MSCs showed the same and high alkaline phosphatase activity, both in flight and in the control groups, suggesting no effect of spaceflight factors on early

  7. [Establishment and evaluation of review criteria for ADVIA 120/2120 and different series of hematology analyzers]. (United States)

    Xing, Ying; Wang, Jian-Zhong; Pu, Cheng-Wei; Shang, Ke; Yan, Zhen-Lin; Bai, Yu-Zhu; Yang, Wen-Shuang; Li, Tao; Zhang, Shi-Min


    To establish the suitable review criteria for ADVIA 120/2120 and those for different series of hematology analyzers. A total of 2400 samples, including 6 blood neoplasms, were detected with ADVIA 120/2120 hematology analyzer, in which 1200 samples were detected by Sysmex XE-2100 and Beckman-Coulter LH750 hematology analyzers. In the meantime, blood smears were reviewed, and the results were analyzed statistically. The new review criteria were established by consulting and modifying the one as recommended by an international consensus group. Finally 300 samples were selected to validate the new review criteria. The results of 2400 samples detected by ADVIA 120/2120 hematology analyzer were analyzed statistically according to the international consensus review rules and blood smear positive criteria formulated by Chinese experts. The true positive rate was 22.1% (n = 530), false positive rate 28.1% (n = 675), true negative rate 44.3% (n = 1063), false negative rate 5.5% (n = 132), and the smear review rate 50.2% (n = 1205). The false negative rate was over the acceptable limit of 5%. The new review criteria were established by amending the blood smear positive criteria, i. e. increasing the percentage of band neutrophils, eosinophils, basophils and monocytes and adjusting the international consensus review rules. Then the results were re-analyzed. The true positive rate, false positive rate, true negative rate and false negative rate were 15.5% (n = 371), 18.7% (n = 449), 61.6% (n = 1479) and 4.2% (n = 101) respectively. The smear review rate was 34.2% (n = 821) and no specimen of blood neoplasms was missed. On that basis, the current review criteria for ADVIA 120/2120, XE-2100 and LH750 hematology analyzer were proposed by adding some specific parameters. The results of 1200 samples with three instruments were analyzed according to the current criteria. And the false negative rates were 4.3%, 4.6% and 4.6%, and false positive rate 14.7%, 17.5% and 12.7% respectively

  8. Comparison and evaluation of seven different bench-top flow cytometers with a modified six-plexed mycotoxin kit. (United States)

    Czeh, Arpad; Schwartz, Abe; Mandy, Frank; Szoke, Zsuzsanna; Koszegi, Balazs; Feher-Toth, Szilvia; Nagyeri, Gyorgy; Jakso, Pal; Katona, Robert L; Kemeny, Agnes; Woth, Gabor; Lustyik, Gyorgy


    Many bench-top flow cytometers (b-FCs) are compatible with microsphere-based multiplexed assays. Disciplines implementing b-FCs-based assays are expanding; they include monitoring and validating food quality. A multiplexed platform protocol was evaluated for poly-mycotoxin assays, which is compatible with a variety of b-FC models. The seven instruments included: BD FACSCalibur(™) , BD FACSArray(™) Bioanalyzer, Accuri C6, Partec CyFlow(®) Space, Beckman Coulter FC 500, Guava EasyCyte Mini, and Luminex 100 (™) . Current reports related to the food industry describe fungal co-infections leading to poly-mycotoxin contamination in grain (Sulyok M, Berthiller F, Krska R, Schuhmacher R, Rapid Commun Mass Spectrom 2006;20:2649-2659). It is imperative to determine whether b-FC-based assays can replace traditional single-mycotoxin enzyme-linked immunosorbent assay (ELISA). A six-plexed poly-mycotoxin kit was tested on seven different b-FCs. The modified kit was initially developed for the BD FACSArray(™) Bioanalyzer (BD Biosciences) (Czeh A, Mandy F, Feher-Toth S, Torok L, Mike Z, Koszegi B, Lustyik G, J Immunol Methods 2012;384:71-80). With the multiplexed platform, it is possible to identify up to six mycotoxin contaminants simultaneously at regional grain collection/transfer/inspection facilities. In the future, elimination of contaminated food threat may be better achieved with the inclusion of b-FCs in the food protection arsenal. A universal protocol, matched with postacquisition software, offers an effective alternative platform compared to using a series of ELISA kits. To support side-by-side evaluation of seven flow cytometers, an instrument-independent fluorescence emission calibration was added to the protocol. All instrument performances were evaluated for strength of agreement based on paired sets of evaluation to predicate method. The results suggest that all b-FCs were acceptable of performing with the multiplexed kit for five of six mycotoxins. For

  9. TSH-CHECK-1 test: diagnostic accuracy and potential application to initiating treatment for hypothyroidism in patients on anti-tuberculosis drugs.

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    Cara S Kosack

    Full Text Available BACKGROUND: Thyroid-stimulating hormone (TSH promotes expression of thyroid hormones which are essential for metabolism, growth, and development. Second-line drugs to treat tuberculosis (TB can cause hypothyroidism by suppressing thyroid hormone synthesis. Therefore, TSH levels are routinely measured in TB patients receiving second-line drugs, and thyroxin treatment is initiated where indicated. However, standard TSH tests are technically demanding for many low-resource settings where TB is prevalent; a simple and inexpensive test is urgently needed. METHODS: As a proof of concept study TSH was measured in routinely collected sera at the University Medical Center Utrecht, Netherlands, using the TSH-CHECK-1 (VEDALAB, Alençon, France, a lateral-flow rapid immunochromatographic assay with a TSH cut-off value of 10 µIU/mL, the standard threshold for initiating treatment. These results were compared with TSH levels measured by a reference standard (UniCel DXi 800 imunoassay system, Beckman Coulter, USA. Sensitivity, specificity, and likelihood ratios were then calculated. RESULTS: A total of 215 serum samples were evaluated: 107 with TSH values <10 µIU/mL and 108 with values ≥10 µIU/mL. TSH-CHECK-1 test sensitivity was found to be 100.0% (95% CI: 96.6-100.0 and specificity was 76.6% (95% CI: 67.5-84.3. Predictive values (PV were modelled for different levels of prevalence. For a prevalence of 10% and 50%, the positive PV was 32.2% (95% CI: 25.0-39.7% and 81.1% (95% CI: 75.0-85.5%, respectively; the negative PV was 100% (95% CI: 98.9-100% and 100% (95% CI: 91.3-100% respectively. DISCUSSION/CONCLUSIONS: The TSH-CHECK-1 rapid test was practical and simple to perform but difficult to interpret on weak positive results. All sera with TSH≥10 µIU/mL were correctly identified, but the test lacked sufficient specificity. Given its excellent negative PV in this evaluation, the test shows promise for ruling out hypothyroidism. However, so far it

  10. Point-of-Care Versus Central Laboratory Measurements of Hemoglobin, Hematocrit, Glucose, Bicarbonate and Electrolytes: A Prospective Observational Study in Critically Ill Patients.

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    Jérôme Allardet-Servent

    Full Text Available Rapid detection of abnormal biological values using point-of-care (POC testing allows clinicians to promptly initiate therapy; however, there are concerns regarding the reliability of POC measurements. We investigated the agreement between the latest generation blood gas analyzer and central laboratory measurements of electrolytes, bicarbonate, hemoglobin, hematocrit, and glucose.314 paired samples were collected prospectively from 51 critically ill patients. All samples were drawn simultaneously in the morning from an arterial line. BD Vacutainer tubes were analyzed in the central laboratory using Beckman Coulter analyzers (AU 5800 and DxH 800. BD Preset 3 ml heparinized-syringes were analyzed immediately in the ICU using the POC Siemens RAPIDPoint 500 blood gas system. We used CLIA proficiency testing criteria to define acceptable analytical performance and interchangeability.Biases, limits of agreement (±1.96 SD and coefficients of correlation were respectively: 1.3 (-2.2 to 4.8 mmol/L, r = 0.936 for sodium; 0.2 (-0.2 to 0.6 mmol/L, r = 0.944 for potassium; -0.9 (-3.7 to 2 mmol/L, r = 0.967 for chloride; 0.8 (-1.9 to 3.4 mmol/L, r = 0.968 for bicarbonate; -11 (-30 to 9 mg/dL, r = 0.972 for glucose; -0.8 (-1.4 to -0.2 g/dL, r = 0.985 for hemoglobin; and -1.1 (-2.9 to 0.7%, r = 0.981 for hematocrit. All differences were below CLIA cut-off values, except for hemoglobin.Compared to central Laboratory analyzers, the POC Siemens RAPIDPoint 500 blood gas system satisfied the CLIA criteria of interchangeability for all tested parameters, except for hemoglobin. These results are warranted for our own procedures and devices. Bearing these restrictions, we recommend clinicians to initiate an appropriate therapy based on POC testing without awaiting a control measurement.

  11. Cytokine responses in the Japanese pufferfish (Takifugu rubripes) head kidney cells induced with heat-killed probiotics isolated from the Mongolian dairy products. (United States)

    Biswas, G; Korenaga, H; Nagamine, R; Takayama, H; Kawahara, S; Takeda, S; Kikuchi, Y; Dashnyam, B; Kono, T; Sakai, M


    Cytokine responses in the Japanese pufferfish (Takifugu rubripes) head kidney (HK) cells to heat-killed lactic acid bacteria probiotics isolated from the Mongolian dairy products were investigated by transcriptomic examination. The HK cells were incubated with two heat-killed bacteria, namely Lactobacillus paracasei spp. paracasei (strain 06TCa22) and L. plantarum (strain 06CC2) and the responses of 16 cytokine genes at 0 (control), 1, 4, 8, 12, 24 and 48 h post-stimulation were assayed by multiplex RT-PCR analysis (GenomeLab Genetic Analysis System, GeXPS; Beckman Coulter, Inc.). The 16 genes included in the assay were pro-inflammatory cytokines (IL-1β, IL-6, IL-17A/F-3, TNF-α and TNF-N), cell-mediated immune regulators (IL-12p35, IL-12p40 and IL-18), antiviral (I-IFN-1 and IFN-γ) and other regulatory (IL-2, IL-7, IL-15, IL-21, IL-10 and TGF-β1) cytokines. Despite the differences in the transcriptional profiles, expression of all the cytokines tested here was significantly elevated by both the probiotic bacterial stimulants compared with the unstimulated control. Therefore, this in vitro study has demonstrated the modulation of cytokine defense mechanisms in the HK cells by the two heat-killed probiotics indicating their potentiality as novel immunostimulants to fish. However, strain-dependent varied expression of important cytokines (cell-mediated immune regulators, antiviral and anti-inflammatory cytokines) suggests better efficacy of L. paracasei spp. paracasei strain as fish immunostimulant. Further in vivo studies to elucidate the cytokine regulation networks will validate our present observations. A careful evaluation of ant-inflammatory properties may be undertaken using single strain to affirm the immunostimulatory capability. Moreover, application timings and frequency to assess the longevity of immunostimulant effects and to make the application cost-effective need to be evaluated before any practical use in aquaculture. Copyright © 2013 Elsevier

  12. Standardization of 8-color flow cytometry across different flow cytometer instruments: A feasibility study in clinical laboratories in Switzerland. (United States)

    Glier, Hana; Heijnen, Ingmar; Hauwel, Mathieu; Dirks, Jan; Quarroz, Stéphane; Lehmann, Thomas; Rovo, Alicia; Arn, Kornelius; Matthes, Thomas; Hogan, Cassandra; Keller, Peter; Dudkiewicz, Ewa; Stüssi, Georg; Fernandez, Paula


    The EuroFlow Consortium developed a fully standardized flow cytometric approach from instrument settings, through antibody panel, reagents and sample preparation protocols, to data acquisition and analysis. The Swiss Cytometry Society (SCS) promoted a study to evaluate the feasibility of using such standardized measurements of 8-color data across two different flow cytometry platforms - Becton Dickinson (BD) FACSCanto II and Beckman Coulter (BC) Navios, aiming at increasing reproducibility and inter-laboratory comparability of immunophenotypic data in clinical laboratories in Switzerland. The study was performed in two phases, i.e. a learning phase (round 1) and an analytical phase (rounds 2 and 3) consisting of a total of three rounds. Overall, 10 laboratories using BD FACSCanto II (n=6) or BC Navios (n=4) flow cytometers participated. Each laboratory measured peripheral blood samples from healthy donors stained with a uniform antibody panel of reagents - EuroFlow Lymphoid Screening Tube (LST) - applying the EuroFlow standardized protocols for instrument setup and sample preparation ( All data files were analyzed centrally and median fluorescence intensity (MedFI) values for individual markers on defined lymphocyte subsets were recorded; variability from reference MedFI values was assessed using performance scores. Data troubleshooting and discussion of the results with the participants followed after each round at SCS meetings. The results of the learning phase demonstrated that standardized instrument setup and data acquisition are feasible in routine clinical laboratories without previous experience with EuroFlow. During the analytical phase, highly comparable data were obtained at the different laboratories using either BD FACSCanto II or BC Navios. The coefficient of variation of MedFI for 7 of 11 markers performed repeatedly below 30%. In the last study round, 89% of participants scored over 90% MedFI values within the acceptance criteria

  13. The BOC ELISA, a ruminant-specific AMH immunoassay, improves the determination of plasma AMH concentration and its correlation with embryo production in cattle. (United States)

    Arouche, Nassim; Picard, Jean-Yves; Monniaux, Danielle; Jamin, Soazik P; Vigier, Bernard; Josso, Nathalie; Cate, Richard L; di Clemente, Nathalie; Taieb, Joëlle


    Plasma anti-Müllerian hormone (AMH) concentrations have been recently found to be predictive of the number of embryos recovered after FSH superovulatory treatment in the cow. However, the sensitivity of the Active Müllerian-inhibiting substance/AMH ELISA (ref. 10-14400; DSL-Beckman-Coulter) used to make these measurements in bovine plasma samples is low because it was developed to measure human AMH levels. To overcome this limitation, we developed an immunoassay specific for the bovine (B), ovine (O), and caprine (C) species, the bovine-ovine-caprine (BOC) ELISA. For this purpose, we produced recombinant bovine AMH for standardization, and we used monoclonal antibodies raised against bovine AMH, previously prepared by our laboratory. We evaluated the precision, accuracy, specificity, limit of detection, and functional sensitivity of the assay. The intra-assay coefficient of variation ranged between 3.4% and 11.3% for AMH concentrations between 23.68 and 1.74 ng/mL, and the interassay coefficient of variation ranged between 4.8% and 20.5% for concentrations between 25.53 and 1.42 ng/mL, respectively. The assay displayed a good linearity, had a detection limit of 0.4 ng/mL and a functional sensitivity of 1.4 ng/mL. It also cross-reacted with ovine and caprine AMHs. Both the mean and median AMH levels measured in 40 cow plasma samples using the BOC ELISA were approximately 44 fold higher than the mean and median AMH levels measured with the Active Müllerian-inhibiting substance/AMH ELISA. Moreover, a higher correlation was observed between the average number of embryos recovered from each cow after superovulatory treatment and AMH concentrations measured with the BOC ELISA. This BOC ELISA provides a very efficient tool for evaluating the ovarian follicular reserve of cows and predicting their embryo production capacity. Copyright © 2015 Elsevier Inc. All rights reserved.

  14. Upregulation of genes related to bone formation by γ-amino butyric acid and γ-oryzanol in germinated brown rice is via the activation of GABAB-receptors and reduction of serum IL-6 in rats. (United States)

    Muhammad, Sani Ismaila; Maznah, Ismail; Mahmud, Rozi; Zuki, Abu Bakar Zakaria; Imam, Mustapha Umar


    Osteoporosis and other bone degenerative diseases are among the most challenging non-communicable diseases to treat. Previous works relate bone loss due to osteoporosis with oxidative stress generated by free radicals and inflammatory cytokines. Alternative therapy to hormone replacement has been an area of interest to researchers for almost three decades due to hormone therapy-associated side effects. In this study, we investigated the effects of gamma-amino butyric acid (GABA), gamma-oryzanol (ORZ), acylated steryl glucosides (ASG), and phenolic extracts from germinated brown rice (GBR) on the expression of genes related to bone metabolism, such as bone morphogenic protein-2 (BMP-2), secreted protein acidic and rich in cysteine (SPARC), runt-related transcription factor 2 (RUNX-2), osteoblast-specific transcription factor osterix (Osx), periostin, osteoblast specific factor (Postn), collagen 1&2 (Col1&2), calcitonin receptor gene (CGRP); body weight measurement and also serum interleukin-6 (IL-6) and osteocalcin, in serum and bone. Rats were treated with GBR, ORZ, GABA, and ASG at (100 and 200 mg/kg); estrogen (0.2 mg/kg), or remifemin (10 and 20 mg/kg), compared to ovariectomized non-treated group as well as non-ovariectomized non-treated (sham) group. Enzyme-linked immunosorbent assay was used to measure the IL-6 and osteocalcin levels at week 2, 4, and 8, while the gene expression in the bone tissue was determined using the Genetic Analysis System (Beckman Coulter Inc., Brea, CA, USA). The results indicate that groups treated with GABA (100 and 200 mg/kg) showed significant upregulation of SPARC, calcitonin receptor, and BMP-2 genes (P < 0.05), while the ORZ-treated group (100 and 200 mg/kg) revealed significant (P < 0.05) upregulation of Osx, Postn, RUNX-2, and Col1&2. Similarly, IL-6 concentration decreased, while osteocalcin levels increased significantly (P < 0.05) in the treated groups as compared to ovariectomized non-treated groups. GABA and ORZ from

  15. Bicentric evaluation of six anti-toxoplasma immunoglobulin G (IgG) automated immunoassays and comparison to the Toxo II IgG Western blot. (United States)

    Maudry, Arnaud; Chene, Gautier; Chatelain, Rémi; Patural, Hugues; Bellete, Bahrie; Tisseur, Bernard; Hafid, Jamal; Raberin, Hélène; Beretta, Sophie; Sung, Roger Tran Manh; Belot, Georges; Flori, Pierre


    A comparative study of the Toxoplasma IgG(I) and IgG(II) Access (Access I and II, respectively; Beckman Coulter Inc.), AxSYM Toxo IgG (AxSYM; Abbott Diagnostics), Vidas Toxo IgG (Vidas; bioMerieux, Marcy l'Etoile, France), Immulite Toxo IgG (Immulite; Siemens Healthcare Diagnostics Inc.), and Modular Toxo IgG (Modular; Roche Diagnostics, Basel, Switzerland) tests was done with 406 consecutive serum samples. The Toxo II IgG Western blot (LDBio, Lyon, France) was used as a reference technique in the case of intertechnique discordance. Of the 406 serum samples tested, the results for 35 were discordant by the different techniques. Using the 175 serum samples with positive results, we evaluated the standardization of the titrations obtained (in IU/ml); the medians (second quartiles) obtained were 9.1 IU/ml for the AxSYM test, 21 IU/ml for the Access I test, 25.7 IU/ml for the Access II test, 32 IU/ml for the Vidas test, 34.6 IU/ml for the Immulite test, and 248 IU/ml for the Modular test. For all the immunoassays tested, the following relative sensitivity and specificity values were found: 89.7 to 100% for the Access II test, 89.7 to 99.6% for the Immulite test, 90.2 to 99.6% for the AxSYM test, 91.4 to 99.6% for the Vidas test, 94.8 to 99.6% for the Access I test, and 98.3 to 98.7% for the Modular test. Among the 406 serum samples, we did not find any false-positive values by two different tests for the same serum sample. Except for the Modular test, which prioritized sensitivity, it appears that the positive cutoff values suggested by the pharmaceutical companies are very high (either for economical or for safety reasons). This led to imperfect sensitivity, a large number of unnecessary serological follow-ups of pregnant women, and difficulty in determining the serological status of immunosuppressed individuals.

  16. Point-of-Care Hemoglobin/Hematocrit Testing: Comparison of Methodology and Technology. (United States)

    Maslow, Andrew; Bert, Arthur; Singh, Arun; Sweeney, Joseph


    Point-of-care (POC) testing allows rapid assessment of hemoglobin (Hgb) and hematocrit (Hct) values. This study compared 3 POC testing devices--the Radical-7 pulse oximeter (Radical-7, Neuchȃtel, Switzerland), the i-STAT (Abbott Point of Care, Princeton, NJ), and the GEM 4000 (Instrumentation Laboratory, Bedford, MA)--to the hospital reference device, the UniCel DxH 800 (Beckman Coulter, Brea, CA) in cardiac surgery patients. Prospective study. Tertiary care cardiovascular center. Twenty-four consecutive elective adult cardiac surgery patients. Hgb and Hct values were measured using 3 POC devices (the Radical-7, i-STAT, and GEM 4000) and a reference laboratory device (UniCel DxH 800). Data were collected simultaneously before surgery, after heparin administration, after heparin reversal with protamine, and after sternal closure. Data were analyzed using bias analyses. POC testing data were compared with that of the reference laboratory device. Hgb levels ranged from 6.8 to 15.1 g/dL, and Hct levels ranged from 20.1% to 43.8%. The overall mean bias was lowest with the i-STAT (Hct, 0.22%; Hgb 0.05 g/dL) compared with the GEM 4000 (Hct, 2.15%; Hgb, 0.63 g/dL) and the Radical-7 (Hgb 1.16 g/dL). The range of data for the i-STAT and Radical-7 was larger than that with the GEM 4000, and the pattern or slopes changed significantly with the i-STAT and Radical-7, whereas that of the GEM 4000 remained relatively stable. The GEM 4000 demonstrated a consistent overestimation of laboratory data, which tended to improve after bypass and at lower Hct/Hgb levels. The i-STAT bias changed from overestimation to underestimation, the latter in the post-cardiopulmonary bypass period and at lower Hct/Hgb levels. By contrast, the Radical-7 biases increased during the surgical procedure and in the lower ranges of Hgb. Important clinical differences and limitations were found among the 3 POC testing devices that should caution clinicians from relying on these data as sole determinants of

  17. Point-of-Care Versus Central Laboratory Measurements of Hemoglobin, Hematocrit, Glucose, Bicarbonate and Electrolytes: A Prospective Observational Study in Critically Ill Patients. (United States)

    Allardet-Servent, Jérôme; Lebsir, Melissa; Dubroca, Christian; Fabrigoule, Martine; Jordana, Sylvie; Signouret, Thomas; Castanier, Matthias; Thomas, Guillemette; Soundaravelou, Rettinavelou; Lepidi, Anne; Delapierre, Laurence; Penaranda, Guillaume; Halfon, Philippe; Seghboyan, Jean-Marie


    Rapid detection of abnormal biological values using point-of-care (POC) testing allows clinicians to promptly initiate therapy; however, there are concerns regarding the reliability of POC measurements. We investigated the agreement between the latest generation blood gas analyzer and central laboratory measurements of electrolytes, bicarbonate, hemoglobin, hematocrit, and glucose. 314 paired samples were collected prospectively from 51 critically ill patients. All samples were drawn simultaneously in the morning from an arterial line. BD Vacutainer tubes were analyzed in the central laboratory using Beckman Coulter analyzers (AU 5800 and DxH 800). BD Preset 3 ml heparinized-syringes were analyzed immediately in the ICU using the POC Siemens RAPIDPoint 500 blood gas system. We used CLIA proficiency testing criteria to define acceptable analytical performance and interchangeability. Biases, limits of agreement (±1.96 SD) and coefficients of correlation were respectively: 1.3 (-2.2 to 4.8 mmol/L, r = 0.936) for sodium; 0.2 (-0.2 to 0.6 mmol/L, r = 0.944) for potassium; -0.9 (-3.7 to 2 mmol/L, r = 0.967) for chloride; 0.8 (-1.9 to 3.4 mmol/L, r = 0.968) for bicarbonate; -11 (-30 to 9 mg/dL, r = 0.972) for glucose; -0.8 (-1.4 to -0.2 g/dL, r = 0.985) for hemoglobin; and -1.1 (-2.9 to 0.7%, r = 0.981) for hematocrit. All differences were below CLIA cut-off values, except for hemoglobin. Compared to central Laboratory analyzers, the POC Siemens RAPIDPoint 500 blood gas system satisfied the CLIA criteria of interchangeability for all tested parameters, except for hemoglobin. These results are warranted for our own procedures and devices. Bearing these restrictions, we recommend clinicians to initiate an appropriate therapy based on POC testing without awaiting a control measurement.

  18. Promoting Enterprise Success within Team Based Entities in Hierarchical Organizations: A Study of Business Systems Modernization (BSM) Initiatives at Defense Supply Center Richmond (DSCR) (United States)


    23 e. Sustaining Change..................................................................24 F. RICHARD BECKMAN AND REUBEN T. HARRIS...entities and will be incorporated, almost in its entirety, within the researcher’s hypothesized model. 25 F. RICHARD BECKMAN AND REUBEN T. HARRIS...Analysis Results 215 LIST OF REFERENCES Beckhard , R., & Harris, R. T. (1987). Organizational transitions: Managing complex change (2nd ed.). Reading

  19. The Role of Anticoagulation in the Measurement of Platelet Volumes. (United States)


    Haematol. 25:675-689. 5. Coulter Electronics. 1978. Coulter Counter Model S-Plus Instruction Manual . Hialeah, FL. 6. Cowan, D. H., A. L. Robertson, P...platelet function. Thromb. Diath. Haemorrh., 33:63-65. 23. Zucker, M. B. and J. Borrelli . 1954. Reversible alterations in platelet morphology produced

  20. Population Based Assessment of MHC Class I Antigens Down Regulation as Markers of Increased Risk for Development and Progression of Breast Cancer from Benign Breast Lesions (United States)


    MCC cytometer using the Coulter System II – software v 3.0 (Coulter, Miami, Florida, USA). The mean fluorescence intensity (MFI) from samples was noted...interpretation of low signal allele peaks, stutter peaks and difficulty with amplification of larger size alleles due to degradation of DNA inherent

  1. Study of urinary leukotriene E4 in atopic dermatitis: relation to ...

    African Journals Online (AJOL)


    I-Complete blood count using Coulter cell counter. T660 (Coulter corporation, Florida, USA) with examination ... The results displayed are corrected values. IV- Quantitative determination of serum IgE concentrations: 2 ml of venous blood were collected in a tube, left to clot and centrifuged at. 1500 rpm to separate the serum.

  2. Download this PDF file

    African Journals Online (AJOL)


    Sep 1, 2002 ... Haemoglobin (Hb) was estimated using a portable. HemoCue ... Laboratory at Muhimbili Medical Centre for analysis. Specimens were processed within three hours of collection. Haematological analysis was done by a technician using the. Coulter electronic counter system (Coulter-Electronic Ltd).

  3. History of the chemical heritage foundation scientific instrumentation museum.

    Energy Technology Data Exchange (ETDEWEB)

    Ferraro, J. R.; Brame, E. G., Jr.; Chemistry; Cecon Group, Inc.


    It all began in March 1990 at the 40th Pittsburgh Conference (PittCon) meeting in the Jacob Javitz Convention Center in New York, New York. Coauthor John R. Ferraro stopped at the Beckman Booth and began discussing with Robert Jarnutowski, at the time an engineer with Beckman Instruments (Fullerton, CA), the impending 50th anniversary of the landmark instrument, the Beckman DU spectrophotometer, in 1991. The thought entered Ferraro's mind that landmark instruments such as this one should be preserved in a museum, Germany, England, and Italy host scientific instrumentation museums.

  4. Download this PDF file

    African Journals Online (AJOL)

    —. 650 cnfl) were recorded on a Perkin Elmer 25'? spectrophotometer and far. IR measurements were carried out on a Beckman lit-12 spectrophotometer. All manipulations were carried out in a dry box under an atmosphere of dry nitrogen.

  5. Physiological changes during the ovarian cycle of the female rock ...

    African Journals Online (AJOL)

    Seasonal morphometriC and physiological changes associated .... Plasma protein fractions were separated on cellulose acetate membranes using a Beckman microzone electrophoresis system. A human plasma sample was separated concurrently on each ..... reached concerning secondary medullary bone fonnation.

  6. The DOE fellows program-a workforce development initiative for the US department of energy

    International Nuclear Information System (INIS)

    Lagos, Leonel E.


    posters and 7 oral presentations given at WM. The DOE Fellows participation at WM has resulted in three Best Student Poster Awards (WM09, WM10, and WM11) and one Best Professional Poster Award (WM09). DOE Fellows have also presented their research at ANS DD and R and ANS Robotics Topical meetings and this year two Fellows will present at the International Conference on Environmental Remediation and Radioactive Waste Management (ICEM13) in Brussels, Belgium. Moreover, several of our DOE Fellows have already obtained employment with DOE-EM, other federal agencies, DOE contractors, commercial nuclear power companies, and other STEM industry (GE, Boeing, Lockheed Martin, Johnson and Johnson, Beckman-Coulter, and other top companies). This paper will discuss how DOE Fellows program is training and mentoring FIU STEM students in Department of Energy's Office of Environmental Management technical challenges and research. This training and mentoring has resulted in the development of well-trained and polished young scientists and engineers that will become the future workforce in charge of carrying on DOE-EM's environmental cleanup mission. The paper will showcase FIU's DOE Fellows model and highlight some of the applied research the DOE Fellows have conducted at FIU's Applied Research Center and across the DOE Complex by participating in summer internship assignments. (authors)

  7. A convenient fluorometric method to study sulfur mustard-induced apoptosis in human epidermal keratinocytes monolayer microplate culture. (United States)

    Ray, Radharaman; Hauck, Stephanie; Kramer, Rachel; Benton, Betty


    -Asp-Glu-Val-Asp-fluorochrome, also designated as AC-or Z-DEVD- fluorochrome) added to the assay medium. Fluorescence was measured using a plate reader. We used two types of substrates, one (Sigma-Aldrich, CASP-3-F) required cell disruption and the other (Beckman-Coulter CellProbe HT Caspase-3/7 Whole Cell Assay Kit) was cell permeable. The latter substrate was useful in experiments such as determining the time-course of apoptosis immediately following SM exposure without disruption (e.g., due to cell processing). In SM-exposed HEK, fluorescence generated from the fluorogenic caspase-3 substrate hydrolysis increased in a time (0-24 h) and concentration (0.05, 0.1, 0.15, 0.2, 0.3, 0.5 mM) dependent manner. SM caused maximum fluorescence at about 0.5 mM. However, at 2 mM SM, fluorescence decreased compared with 0.5 mM, which remains to be explained. Following 0.3 mM SM exposure, which is considered to be the in vitro equivalent of a vesicating dose in vivo (Smith, W. J., Sanders, K. M., Ruddle, S. E., Gross, C. L. (1993). Cytometric analysis of DNA changes induced by sulfur mustard. J. Toxicol.-Cut. Ocular Toxicol. 12(4):337-347.), a small fluorescence increase was observed at 6 to 8 h, which was markedly higher at 12 h. At 24 h, all SM concentrations increased fluorescence. Fluorescence increase due to SM was prevented 100% by a caspase-3-specific peptide inhibitor AC-DEVD-CHO (acetyl-Asp-Glu-Val-Asp-aldehyde, 0.1 mM), but less effectively by a general caspase inhibitor Z-VAD-FMK (benzyl oxycarbonyl-Val-Ala-Asp-fluoromethylketone, 0.01 mM), indicating that the fluorescence increase was due to caspase-3-mediated apoptosis. These results suggest potential applications of this method to study apoptosis mechanisms involving caspase-3 substrates and possibly those involving other caspase substrates.

  8. The percentage of prostate-specific antigen (PSA) isoform [-2]proPSA and the Prostate Health Index improve the diagnostic accuracy for clinically relevant prostate cancer at initial and repeat biopsy compared with total PSA and percentage free PSA in men aged ≤65 years. (United States)

    Boegemann, Martin; Stephan, Carsten; Cammann, Henning; Vincendeau, Sébastien; Houlgatte, Alain; Jung, Klaus; Blanchet, Jean-Sebastien; Semjonow, Axel


    To prospectively test the diagnostic accuracy of the percentage of prostate specific antigen (PSA) isoform [-2]proPSA (%p2PSA) and the Prostate Health Index (PHI), and to determine their role for discrimination between significant and insignificant prostate cancer at initial and repeat prostate biopsy in men aged ≤65 years. The diagnostic performance of %p2PSA and PHI were evaluated in a multicentre study. In all, 769 men aged ≤65 years scheduled for initial or repeat prostate biopsy were recruited in four sites based on a total PSA (t-PSA) level of 1.6-8.0 ng/mL World Health Organization (WHO) calibrated (2-10 ng/mL Hybritech-calibrated). Serum samples were measured for the concentration of t-PSA, free PSA (f-PSA) and p2PSA with Beckman Coulter immunoassays on Access-2 or DxI800 instruments. PHI was calculated as (p2PSA/f-PSA × √t-PSA). Uni- and multivariable logistic regression models and an artificial neural network (ANN) were complemented by decision curve analysis (DCA). In univariate analysis %p2PSA and PHI were the best predictors of prostate cancer detection in all patients (area under the curve [AUC] 0.72 and 0.73, respectively), at initial (AUC 0.67 and 0.69) and repeat biopsy (AUC 0.74 and 0.74). t-PSA and %f-PSA performed less accurately for all patients (AUC 0.54 and 0.62). For detection of significant prostate cancer (based on Prostate Cancer Research International Active Surveillance [PRIAS] criteria) the %p2PSA and PHI equally demonstrated best performance (AUC 0.70 and 0.73) compared with t-PSA and %f-PSA (AUC 0.54 and 0.59). In multivariate analysis PHI we added to a base model of age, prostate volume, digital rectal examination, t-PSA and %f-PSA. PHI was strongest in predicting prostate cancer in all patients, at initial and repeat biopsy and for significant prostate cancer (AUC 0.73, 0.68, 0.78 and 0.72, respectively). In DCA for all patients the ANN showed the broadest threshold probability and best net benefit. PHI as single parameter

  9. Lessons learned from a case of tert-butyl glucuronide excretion in urine - "New" psychoactive alcohols knocking on the back door? (United States)

    Arndt, Torsten; Buschmann, Hubert C; Schulz, Katja; Stemmerich, Karsten


    Ethyl glucuronide (EtG) in urine is considered a marker of recent ethanol consumption or ethanol exposition. tert-Butanol is primarily used as a solvent and intermediate chemical. Like tert-amyl alcohol, tert-butanol is discussed in Internet forums as ethanol replacement. We discuss false-positive immunological EtG screenings by excretion of different alcohol glucuronides (EtG homologs), mainly tert-butyl glucuronide in urine of a polytoxikomanic in-patient. Three consecutive urine samples from an in-patient with a long history of multiple substance abuse including solvents were analyzed by DRI EtG enzyme immunoassay (ThermoFisher Scientific Microgenics) on a Beckman Coulter AU680 analyzer, an in-house LC-MS/MS for EtG, 1-propyl, 2-propyl, 1-butyl, 2-butyl, and tert-butyl glucuronide, and an in-house headspace GC-FID of free congener substances methanol, 1-propanol, 2-butanone, 2-butanol, isobutanol, 1-butanol, 3-methyl-1-butanol, 2-methyl-1-butanol, and additionally for ethanol, acetone, 2-propanol, tert-butanol and 2-methyl-2-butanol. EtG immunoassay yielded two positive urine samples (0.2 and 0.6mg/L or 0.1 and 0.2mg/g creatinine; cut-off 0.1mg/L) which were tested EtG negative by LC-MS/MS (cut-off 0.1mg/L) but positive for tert-butyl glucuronide (3.7 and 27.1mg/L), 2-butyl glucuronide (1.1 and 3.5mg/L), and 2-propyl glucuronide (0.1 and 0.4mg/L). Headspace GC-FID detected tert-butanol (0.97 and 4.01mg/L), methanol (0.96 and 0.62mg/L), 2-butanone (0.84 and 1.65mg/L), and 2-butanol (0.04 and 0.09mg/L), but no ethanol and no 2-methyl-2-butanol. Cross-reaction of EtG homologs, mainly tert-butyl glucuronide after suspected tert-butanol or isobutane abuse, explains the false-positive EtG immunoassay findings. Future investigations could address the usefulness of alcohol glucuronides (EtG homologs) in urine as (a) biomarkers of an exposition to alkans or their corresponding alcohol metabolites and (b) as markers for using "old"-well known alcohols like tert-butanol or

  10. Upregulation of genes related to bone formation by γ-amino butyric acid and γ-oryzanol in germinated brown rice is via the activation of GABAB-receptors and reduction of serum IL-6 in rats

    Directory of Open Access Journals (Sweden)

    Muhammad SI


    bone tissue was determined using the Genetic Analysis System (Beckman Coulter Inc, Brea, CA, USA. Results: The results indicate that groups treated with GABA (100 and 200 mg/kg showed significant upregulation of SPARC, calcitonin receptor, and BMP-2 genes (P < 0.05, while the ORZ-treated group (100 and 200 mg/kg revealed significant (P < 0.05 upregulation of Osx, Postn, RUNX-2, and Col1&2. Similarly, IL-6 concentration decreased, while osteocalcin levels increased significantly (P < 0.05 in the treated groups as compared to ovariectomized non-treated groups. Conclusion: GABA and ORZ from GBR stimulates osteoblastogenesis by upregulation of bone formation genes, possibly via the activation of GABAB receptors and by inhibiting the activity of inflammatory cytokines and reactive oxygen species. Therefore, it could be used effectively in the management of osteoporosis. Keywords: gene expression, GBR-bioactive compounds, osteocalcin, ovariectomized rats

  11. Elevated levels of beta-human chorionic gonadotropin and human placental lactogen between 11-13 week's gestation and subsequent pregnancy complications in Oman i women

    International Nuclear Information System (INIS)

    Krolikowski, Andrzej; Al-Busaidi, Fikra; Al-Wahaibi, Adil


    The association between abnormal levels of maternal serum b-human chorionic gonadotrophin (b-HCG) and human placental lactogen (HPL) measured in early pregnancy and future poor pregnancy outcome is fairly well established. Little is known on how such prognostic information can be used to avoid future complications of pregnancy and improve their outcome. We undertook a prospective study, which was designed to assess the efficiency of maternal serum biochemical markers b-HCG and HPL for the detection of different pregnancy and labor complications. The blood samples were taken from pregnant patients who attended the antenatal clinic at Sultan Qaboos University Hospital in Muscat, Sultanate of Oman, from December 2001 to October 2002. These patients were selected randomly. A total of 200 Omani women, none diabetic, with singleton pregnancies between 11 and 13 weeks gestation were recruited into the study. Gestational age was calculated from the first day of the last menstrual period, unless ultrasonography showed a discrepancy of more than 14 days. Excluded from the study were pregnancies with fetal anomalies, multiple gestations and insulin dependent maternal diabetes. Biochemical analysis of both maternal serum markers was performed in the clinical biochemistry laboratory using an automated immunometric technique supplied by Beckman Coulter for b-HCG and manual radioimmunoassay utilizing a gamma radiation scintillation counter for HPL. Both assays underwent internal, trilevel quality controls. The selected patients were monitored for the following complications: pregnancy induced hypertension (PIH), gestational diabetes, polyhydramnios, antepartum hemorrhage, intrauterine growth retardation (IUGR) (birth weight <10th per centile for gestation), low Apgar score (7 or less) and emergency cesarean section. 75 patients did not develop any pregnancy complications and delivered vaginally. This was our control group. 85 patients developed one of the above mentioned problems

  12. Rome

    Directory of Open Access Journals (Sweden)

    Jan Nelis


    Full Text Available Apted, Michael; Farino, Julia; Coulter, Allen et al. (dirs.; HBO/BBC (prod.; Heller, Bruno; Milius, John; Frankel, David; Macdonald, William J.; Cunningham, Alexandra and Hodges, Adrian (script. United Kingdom, 2005: son., col.

  13. Rome


    Jan Nelis


    Apted, Michael; Farino, Julia; Coulter, Allen et al. (dirs.); HBO/BBC (prod.); Heller, Bruno; Milius, John; Frankel, David; Macdonald, William J.; Cunningham, Alexandra and Hodges, Adrian (script). United Kingdom, 2005: son., col.

  14. ECOHAB: Kamykowski_D- Florida Process Cruise 11/16-19/1998- Karenia brevis counts, biochemistry and behavior (NODC Accession 0000533) (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Abstract: Water bottle samples were collected from selected depths (variable with station) at 44 stations in the study area. Coulter counts in the 14 to 28 um size...

  15. Karussellile väljavalitud / Aita Kivi

    Index Scriptorium Estoniae

    Kivi, Aita, 1954-


    Sisu : Eeva Park. Pääse karussellile; Waris Dirie, Cathleen Miller. Kõrbelill; Colette Davenat. Väljavalitu; Arthur Hailey. Kõrged ametikohad; Mari Saat. Sinikõrguste tuultes; Kogumik "Inimese vägi"; Catherine Coulter. Elu ja surma piiril

  16. 77 FR 75947 - Endangered and Threatened Wildlife and Plants; Reclassification of the Continental U.S. Breeding... (United States)


    ... the Act in 1984, prior to publication of the joint policy of the National Marine Fisheries Service and..., amphibians, reptiles, mammals, birds, and arthropods (Coulter et al. 1999, p. 7). Wood storks forage in a...

  17. ECOHAB: Kamykowski_D- Florida Process Cruise 11/16-19/1998- Karenia brevis counts, biochemistry and behavior (NODC Accession 0000534) (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Abstract: Water bottle samples were collected from selected depths (variable with station) at 14 stations in the study area. Coulter counts in the 14-28 um size...

  18. Substitution of Fingertip Blood for Venous Blood in the Measurement of Hematocrit and Hemoglobin Following Exercise (United States)

    Fahey, Thomas D.; And Others


    Results from comparative testing indicate that fingertip blood is a valid indicator of antecubital venous hematocrit (hct) and hemoglobin (hgb), and that hct ratios determined on the Coulter counter are comparable to those found by the microhematocrit method. (MB)

  19. Download this PDF file

    African Journals Online (AJOL)

    ihab elhakim

    (allergic rhinoconjunctivitis). - Anaphylaxis. - Symptoms and signs suggestive of food allergy including gastrointestinal disorders. Study measurements: Umbilical cord whole blood samples (5 ml) were subjected to complete blood counting especially for eosinophils, basophils and platelets using coulter electronic automated ...

  20. Cloning and homologic analysis of Tpn I gene in silkworm Bombyx ...

    African Journals Online (AJOL)



    Mar 19, 2007 ... calcium conditions, tropomyosin locks the actin site of myosin binding. When the Calcium level increases in the sarcoplasm, Troponin C is able to ... encing was performed using an automatic sequencer: CEQ8000. (Beckman USA). Software and database used for bioinformatic analysis. The primer used to ...

  1. A Antimi crobia al susc from ceptibi Asper lity ass rgillus sessm ...

    African Journals Online (AJOL)



    Jan 2, 2015 ... Production, isolation and purification of the compounds. The organism was allowed to grow in a number of culture flasks of. 500 ml capacity containing ... with 0.1% vanillin sulfate spray reagent followed by heating. Spectral measurement. Ultra-violet (UV) spectra were recorded on a Beckman double beam.

  2. The Impact of Siblings with Disabilities on Their Brothers and Sisters Pursuing a Career in Special Education (United States)

    Marks, Susan Unok; Matson, Amy; Barraza, Lori


    It is well known that growing up with a sibling who has a disability has both positive and negative impacts on the non-disabled sibling (Beckman, 2002; Fisman, Wolf, Ellison, & Freeman, 2000; Gans, 1997; McHugh, 2003). In fact, the concern for ameliorating the potential negative impacts on nondisabled siblings has been a major impetus for the…

  3. morphological features of tyrosine hydroxylase immunoreactive cells ...

    African Journals Online (AJOL)


    glandular cells. TH immunoreactive fibers are considered to be extrinsic in origin, arising from the cell bodies in the celiac ganglia (Beckman 1986). Observations made in the rat have showed that neurones in the celiac ganglia that innervate the pancreas are immunopositive for TH (Hökfelt et al. 1977; Schultzberg et a l .

  4. Development of a Model for Hot-Surface Ignition of Combustible Liquids. (United States)


    assumption is somewhat artificial, it is a useful fiction which results in a reasonable expression for the characteristic mixing time. 6 10- A- ’... -. a...mixture, measuring the concentration of CO2 in the resulting combustion products using a Beckman NOIR analyzer, and calcu- lating the corresponding fuel

  5. Apoptosis of lymphocytes in SLE: the level, correlation with ...

    African Journals Online (AJOL)

    Lymphocytes were isolated from venous blood by method of gradient centrifugation of all the blood through a Ficoll-pak solution. The quantity apoptotic cells was determined in leukocytes by flow cytometry Epics XL-2 (“Beckman Coulter”, USA). Analysis of lymphocyte subpopulations was carried by using two fluorescent ...

  6. Factors associated with elevated blood lead levels in inner ·city ...

    African Journals Online (AJOL)

    cate blood samples were obtained from 20% of the population in order to measure intra- and inter-laboratory variation. Blood lead analyses were carried out using standard methods.6. Following preparation and centrifugation, an atomic absorption spectrophotometer (Beckman 1272, M Model) was used to perform lead ...

  7. HUI2 and HUI3

    African Journals Online (AJOL)

    Beckman JA, Creager MA, Libby P. Diabetes and atherosclerosis: epidemiology, pathophysiology, and management. J Am Med Assoc 2002; 287: 2570–2581. 18. Sacco RL, Boden-Albala B, Abel G: Race-ethnic disparities in the impact of stroke risk factors: the northern Manhattan stroke study. Stroke 2001; 32: 1725–1731.

  8. Neural Networks and Non-Destructive Test/Evaluation Methods (United States)


    backgammon ( Tesauro and Sejnowski, 1988), * Performance of nonlinear signal processing (Lippman and Beckman, 1989; Tamura and Waibel, 1988), " Prediction of...Speech. and Signal Processing, April, 553-556. Tesauro , G., and Sejnowski, T. (1988) "A Neural Network that Learns to Play Backgammon." Neural

  9. Organizational Perceptions of Telecommuting in the Private Sector (United States)

    Galusha, Repps J.


    The Internet has provided more organizations the opportunity to adopt telecommuting as a means to recruit and retain employees, boost productivity, and trim facility costs. This study expands on the work of a previous study by Hoang, Nickerson, Beckman, and Eng, in 2008 which found that private organizations, due to perceptions of organizational…

  10. A one-step separation of human serum high density lipoproteins 2 and 3 by rate-zonal density gradient ultracentrifugation in a swinging bucket rotor

    NARCIS (Netherlands)

    Groot, P.H.E.; Scheek, L.M.; Havekes, L.; Noort, W.L. van; Hooft, F.M. van 't


    A method was developed for the separation of the high density lipoprotein subclasses HDL2 and HDL3 from human serum. Six serum samples are fractionated in a single-step ultracentrifugal procedure using the Beckman (SW-40) swinging bucket rotor. The method is based on a difference in flotation rate

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    African Journals Online (AJOL)

    after continuous stirring with a Teflon stirring bar. The complete cell is represented by the scheme: Ag/AgCI 10.1 M CI, 0.1 M CIO, Liquid membrane | Test solution | SCE. pH measurements of solutions were made with a Beckman Chem Mate pH meter. Procedures. Electrode calibration. Calibrations of electrode responses to ...

  12. Antibacterial activity of chrysophanol isolated from Aloe excelsa ...

    African Journals Online (AJOL)


    against four gram negative and five gram positive bacterial strains. The structures of ... Measurements. Melting points were determined on a Gallenhamp melting point apparatus and were uncorrected. The UV and IR spectra were recorded on Beckman DU-7400 and Perkin Elmer FT-IR spectrometers respectively.


    African Journals Online (AJOL)

    different body constituents and heat production (on the logarithmic scale of measurement) during growth. .... hours, are analysed at the end of a particular collection period by means of a Beckman Model IR 215 B infra-red ... Energy of combustion of milk, feed, faeces and urine was determined with an adiabatic bomb calori-.

  14. Full page fax print

    Indian Academy of Sciences (India)


    spectroscopic instruments except for a Beckman DU Spectrophotometer. With foresight TRG very soon established microanalytical (C,H,N) facilities. He made his mark in the national and international arena through a series of publications on the isolation and structure elucidation of many novel plant constituents – alkaloids, ...

  15. Early infant male circumcision for human immunodeficiency virus ...

    African Journals Online (AJOL)

    Prudence Jarrett


    Jun 24, 2014 ... change in particular the Theory of Planned Behaviour (Ajzen. 1985) which incorporate perceptions of ..... increasing uptake of infant circumcision. There are multiple theories explaining the factors that contribute to .... From Intentions to Actions: A Theory of Planned Behavior. In: J. Kuhl & J. Beckman (Eds.), ...

  16. beskrywing van'n apparaat om gaswisseling oor kort periodes by ...

    African Journals Online (AJOL)

    die lug te droog, stofafsluiter, infrarooi CO2-analiseerder. (Beckman IR02 I 5A) paramagnetiese O2-analiseerder (Beck- man Model E2), rota-meter vir lugvloeimeting en diafragma- pompie. Die diafragmapompie pomp die lug deur die hele lug-analise reeks en weer terug in die kas. Die CO2-analiseer- der se lesings word ...

  17. Abstract

    African Journals Online (AJOL)


    request made by a customer for a reservation of a certain class at time T. Although dynamic .... to both customer reaction upon denied boarding and profit loss. .... Sabanci University. http://www.optimization- Bailey, J. 2007. Bumped fliers and no plan B. The New York Times. Beckman, M.J & Bobkoski, F. 1985.


    African Journals Online (AJOL)

    . 5. Duffie J.A., and Beckman W.A., Solar Energy. Thermal Preocesses, Wiley Interscience,. New York (1974). 6. Klein S.A., Calculation of Monthly Average. Insolation on Tilted Surfaces, Solar Energy,. Vol. 19, pp. 325-329, 1977. Journal of ...

  19. The Quadratic Assignment Problem is Easy for Robinsonian Matrices

    NARCIS (Netherlands)

    Laurent, M.; Seminaroti, M.


    We present a new polynomially solvable case of the Quadratic Assignment Problem in Koopmans-Beckman form QAP(A;B), by showing that the identity permutation is optimal when A and B are respectively a Robinson similarity and dissimilarity matrix and one of A or B is a Toeplitz matrix. A Robinson

  20. The quadratic assignment problem is easy for robinsonian matrices

    NARCIS (Netherlands)

    Laurent, M.; Seminaroti, M.

    We present a new polynomially solvable case of the Quadratic Assignment Problem in Koopmans–Beckman form QAP(A,B), by showing that the identity permutation is optimal when AA and BB are respectively a Robinson similarity and dissimilarity matrix and one of AA or BB is a Toeplitz matrix. A Robinson

  1. The quadratic assignment problem is easy for Robinsonian matrices with Toeplitz structure

    NARCIS (Netherlands)

    M. Laurent (Monique); M. Seminaroti (Matteo)


    htmlabstractWe present a new polynomially solvable case of the Quadratic Assignment Problem in Koopmans-Beckman form QAP(A,B), by showing that the identity permutation is optimal when A and B are respectively a Robinson similarity and dissimilarity matrix and one of A or B is a Toeplitz matrix. A

  2. CAL Community School: An Invitation to Patrons To Participate. 1995-96 Report to the Community. (United States)

    CAL Community School, Latimer, IA.

    This annual report provides information on the CAL Community School, a small K-12 school that serves the communities of Alexander, Coulter, and Latimer, Iowa. The mission of the school is to provide a positive educational environment, maintain high academic standards, and promote the value of life-long learning among students. In addition, the…

  3. Xylem monoterpenes of some hard pines of Western North America: three studies (United States)

    Richard H. Smith


    Monoterpene composition was studied in a number of hard pine species and results were compared with earlier work. (1) Intratree measurements showed strong constancy of composition in both single-stemmed and forked trees of ponderosa, Jeffrey, Coulter, and Jeffrey x ponderosa pines. In grafts of these and other pines, the scion influenced the root stock, but not the...

  4. Dietary iron rural blacks overload In southern African

    African Journals Online (AJOL)


    Sep 15, 1990 ... ferritin, C-reactive protein (CRP), 1'-glutamyltransferase (GGn and serological screening for hepatitis B ... with the condition include: portal fibrosis and cirrhosis of the liver/,J glucose intolerance;4 scurvy and ... or a Coulter Model-S electronic counter with standard cali- bration. The ESR was determined by ...

  5. Application of particle size distributions to total particulate stack samples to estimate PM2.5 and PM10 emission factors for agricultural sources (United States)

    Particle size distributions (PSD) have long been used to more accurately estimate the PM10 fraction of total particulate matter (PM) stack samples taken from agricultural sources. These PSD analyses were typically conducted using a Coulter Counter with 50 micrometer aperture tube. With recent increa...

  6. Is Dysguesia Going to be a Rare or a Common Side‑effect of ...

    African Journals Online (AJOL)

    amlodipine are peripheral edema (8.3%), fatigue (4.5%), dizziness, palpitations, myalgia, stomachache, headache, dyspepsia, nausea, blood disorders, gynecomastia, .... Coulter DM. Eye pain with nifedipine and disturbance of taste with captopril: A mutually controlled study showing a method of postmarketing surveillance.

  7. Making Meaning of Student Activism: Student Activist and Administrator Perspectives (United States)

    Harrison, Laura M.; Mather, Peter C.


    College campuses have experienced a recent resurgence of student activism, particularly in response to some of President Donald Trump's executive orders as well as controversial speakers like Ann Coulter and Milo Yiannopoulous. Student activism presents both challenges and opportunities for higher education leaders seeking to engage productively…

  8. ORIGINAL ARTICLES Prevalence and morphological types of ...

    African Journals Online (AJOL)


    Dec 2, 2009 ... A complete blood count was done on a Coulter counter and a Giemsa-stained thin ... A p-value of <0.05 was considered statistically .... Table III. Findings in the history of anaemic and non-anaemic patients. Variable. Anaemia (N (%)). No anaemia (N (%)). OR (CI) p-value. Fatigue. 176 (69.0). 72 (51.4).

  9. Primary production, nutrients, and size spectra of suspended particles in the southern North Sea

    NARCIS (Netherlands)

    Gieskes, W.W.C.


    The effect of nutrient enrichment from the Rhine on some major characteristics of the phytoplankton ecosystem of Dutch coastal waters was studied with 14C, liquid scintillation and Coulter Counter techniques. The magnitude of primary production in the most eutrophic waters closest to

  10. Effect of Food, Diet and Nutrition on Military Readiness and Preparedness of Army Personnel and Dependents in a Peacetime Environment (United States)


    Law 45CFR46. " -, i0 -- In conducting research utilizing recombinant DNA technology , the investigator(s) adhered to current guidelines promulgated by...3) Diet, Neurotransmitters and Behavior, Chandan Prasad, Ph.D., Principal Investigator, 4) Cardiovascular Helth Promotion for Military Personnel and...Report, Volume 1, Number 7. Applied Technology Associates, Inc. 1989. 18 8. Multidimensional leukocyte differential analysis. Coulter Hematology

  11. The metabolic profiles of HIV-infected and non-infected women in ...

    African Journals Online (AJOL)

    Parameter. Methods and equipment. HIV tests. Human Immunodeficiency Viruses (HIV 1/HIV-2: Recombinant antigens and synthetic peptides) reagent pack (Abbott, Germany, catalogue no. 3D41-20). Total lymphocyte counts Via a full blood count on ethyldimethylacetic acid. (EDTA) blood using a Coulter Microdiff 18 Cell.

  12. An improved technique for the prediction of optimal image resolution ...

    African Journals Online (AJOL)



    Oct 4, 2010 ... soil and climatic conditions, competition between grass and woody vegetation, fire, and anthropogenic factors. (Cloudsley-Thompson, 1979; Mahesh et al. ... different disciplines ranging from soil/geomorphology. (Holden, 2001), agriculture (Coulter et al., 2000), biomass. (Atkinson and Curran, 1997) to land ...

  13. Alteration of Bax-to-Bcl-2 ratio modulates the anticancer activity of ...

    African Journals Online (AJOL)



    Oct 20, 2008 ... regulation of cellular response to DNA-damage, cell cycle .... percentage cell viability were determined using a Coulter counter and trypan blue dye exclusion assay, respectively. Each data point represents the mean ± S.D value of three ... the real-time reaction of the products was checked by melting point.

  14. Pikantsed pajatused / Aita Kivi

    Index Scriptorium Estoniae

    Kivi, Aita, 1954-


    Sisu : Louis Paul Boon. Menuett; Herbjırg Wassmo. Õnne poeg; Jean Stone. Sünnipäevatüdrukud; Jo︠l Schmidt. Constantinus Suure mälestused; Mari Vallisoo. Ainsuse olevik; Catherine Coulter. Märklaud; Ross Macdonald. Mees mulla all

  15. [How to deal with the different serum AMH kits in France in 2017? (United States)

    Peigné, M; Robin, G; Catteau-Jonard, S; Giacobini, P; Dewailly, D; Pigny, P


    Serum AMH measurement became a key element in clinical practice, especially before using Assisted reproductive techniques (ART). However, many AMH kits exist giving different AMH results, leading to a confusion in their interpretation. Until recently, only manual ELISA kits existed (mainly Gen II Beckman, EIA/AMH Immunotech and two Anshlab kits) reporting non-interchangeable results. High and low AMH cut-off values, mainly useful to adapt therapeutics in ART, were different between kits. Since the end of 2014, the arrival of two automatic assays (Access Dxi Beckman and AMH Elecsys Roche) seems to improve the sensitivity and the reproducibility of AMH measurement. It could simplify the interpretation of AMH values and improve our clinical choices. This review synthetizes the main comparisons between the different AMH kits available in 2017 to help clinicians in their daily clinical practice. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  16. The international translational regenerative medicine center. (United States)

    Alexis, Mardi de Veuve; Grinnemo, Karl-Henrik; Jove, Richard


    The International Translational Regenerative Medicine Center, an organizing sponsor of the World Stem Cell Summit 2012, is a global initiative established in 2011 by founding partners Karolinska Institutet (Stockholm, Sweden) and Beckman Research Institute at City of Hope (CA, USA) with a mission to facilitate the acceleration of translational research and medicine on a global scale. Karolinska Institutet, home of the Nobel Prize in Medicine or Physiology, is one of the most prestigious medical research institutions in the world. The Beckman Research Institute/City of Hope is ranked among the leading NIH-designated comprehensive cancer research and treatment institutions in the USA, has the largest academic GMP facility and advanced drug discovery capability, and is a pioneer in diabetes research and treatment.

  17. An Enzymatic Bioassay for Perchlorate (United States)


    idea of using DTT as an antioxidant in the reaction was abandoned. Figure 26. Effect of cysteine on the oxidation of NADH by Pcr in the presence of...oxidized by molecular oxygen. The experiments shown above disproved the hypothesis that antioxidants could be added to the reaction to quench molecular... polypropylene tubes and spun in a Beckman TI-50 centrifuge at 35,000 RPM for 1 hour. The supernatant was retained, and the pellet was discarded

  18. Muscle Stem Cell Therapy for the Treatment of DMD Associated Cardiomyopathy (United States)


    exogenous cells into the heart, is a possible approach by which to repair diseased or injured myocardium and improve cardiac function. Though there are a...cardiomyoplasty, which involves the transplantation of exogenous cells into the heart, is a possible approach by which to repair diseased or injured myocardium... Antioxidation and Immunomodulation in Postnatal Multipotent Stem Cell-Mediated Cardiac Repair. Saparov A, Chen C-W, Beckman SA, Wang Y, Huard J

  19. Topical Application of Liposomal Antioxidants for Protection Against CEES Induced Skin Damage (United States)


    lipid rich tissues (adipose tissues, brain, and skin). Chemical analyses following acute HD exposure show a high accumulation in thigh fat , brain...1993;366:398. 9. Pearson GS. Veterans at risk: the health effects of mustard gas and lewisite. Nature. 1993;365:218. 10. Black RM, Read RW. Biological fate...366. 30. Beckman JS, Koppenol WH. Nitric oxide, superoxide, and peroxynitrite: the good, the bad, and ugly . Am J Physiol. 1996;271:1424–1437. 31

  20. Radioimmunological determination of parathormone in human serum

    International Nuclear Information System (INIS)

    Baret, A.; Morcellet, J.L.; Sauvan, R.; Piechowski, J.


    The radioimmunological determination of h PTH was studied, using reagents available on the market: purified bovine parathormone (b PTH) from the Wilson laboratories used for labelling and calibration, synthesized amino thermal peptide of b PTH (1-34 b PTH) from the Beckman laboratories, bovine antiparathormone guinea-pig antiserum from the Burrough Wellcome laboratories (AS 211/32). The results were analysed on standard curves, together with the seric PTH fractions for normal or renal-insufficient subjects [fr

  1. Prostaglandin synthetase activity of goat vesicular microsomes: Co ...

    Indian Academy of Sciences (India)


    The homogenate was centrifuged for 10 min at 12,000 g in a Sorvall Model. RC 5B centrifuge. The supernatant fraction was passed through a double layer of cheese cloth and centrifuged again at 100,000 g for 1 h in a Beckman Ultracentrifuge. Model L5-50. The resulting pellet was suspended in 2 volumes of 0.1 Μ tris-HCl.

  2. Centrifugal Tensioned Metastable Fluid Detectors for Trace Radiation Sources: Experimental Verification and Military Employment (United States)


    the detection of fast neutrons or alpha particles that are telltale signs of nuclear material, while remaining blind to gamma radiation that could...time. CTMFDs could help in the detection of fast neutrons or alpha particles that are telltale signs of nuclear material, while remaining blind to...sensitive equipment worth thousands of dollars. The Beckman LS 6500 is not likely to be used by DTRA or the military services in an austere environment

  3. To Investigate the Therapeutic Efforts of the COX-2 Inhibitor NS-398 as a Single Agent, and in Combination with Vitamin D, in Vitro and in Vivo (United States)


    pathological states including rheumatoid arthritis, diabetic retinopathy and cancer progres- sion (2). Initiation of angiogenesis (angiogenic switch) from Dr. Rama Natarajan (Gonda Diabetes Center, Beckman Research Institute of City of Hope, Duarte, CA). Transfections and reporter gene... predict antiproliferative effects of vitamin D in human prostate cancer cell lines, Mol. Cell Endocrinol. 126 (1997) 83–90. [12] B.Y. Bao, Y.C. Hu

  4. Traumatic Floating Clavicle: A Case Report and Literature Review


    Gouse, Mohamad; Jacob, Korula Mani; Poonnoose, Pradeep Mathew


    Bipolar fracture dislocations of the clavicle are rare injuries, usually the result of high-energy direct trauma. Since the original description by Porral in 1831, only a handful of individual case reports and case series by Beckman and Sanders have been reported in the literature. Management of these injuries has remained controversial ranging from nonoperative to aggressive surgery. We report on the case of a young army cadet who had a fracture of the lateral end of the clavicle, with an an...

  5. Sexual Assault and Sexual Harassment in the U.S. Military: Annex to Volume 3. Tabular Results from the 2014 RAND Military Workplace Study for Coast Guard Service Members (United States)


    lead Bonnie Ghosh-Dastidar, Ph.D. Craig Martin, M.A. Q Burkhart, M.S. Robin Beckman, M.P.H. Megan Mathews, M.A. Marc Elliott, Ph.D. Project Management...Ph.D., team lead Wayne Hintze, M.S. John Rauch Bryan Davis Lena Watkins Richard Sigman, M.S. Michael Hornbostel, M.S. Project Communications Steve

  6. Sexual Assault and Sexual Harassment in the U.S. Military: Annex to Volume 2. Tabular Results from the 2014 RAND Military Workplace Study for Department of Defense Service Members (United States)


    Dastidar, Ph.D. Marc Elliott, Ph.D. Craig Martin, M.A. Mark Totten, M.S. Q Burkhart, M.S. Robin Beckman, M.P.H. Project Management Kayla M. Williams, M.A...Hintze, M.S. John Rauch Bryan Davis Lena Watkins Richard Sigman, M.S. Michael Hornbostel, M.S. Project Communications Steve Kistler Jeffrey Hiday Barbara

  7. Microbial transformation of nucleosides (United States)

    Lamba, S. S.


    A study involving the use of coulter counter in studying the effects of neomycin on E. coli, S. aureus and A. aerogenes was completed. The purpose of this was to establish proper technique for enumeration of cells per ml. It was found that inhibitory effects on growth of E. coli and A. aerogenes, both gram negative organisms, were directly related to the concentration of neomycin used. However, in case S. aureus, a gram positive organism, a decreased inhibition was noted at higher concentrations. A paper entitled, Use of Coulter Counter in Studying Effect of Drugs on Cells in Culture 1 - Effects of Neomycin on E. coli, S. aureus and A. aerogenes, is attached in the appendix. Laboratory procedures were also established to study the effects of nucleoside antibiotic cordycepin on He La cell grown in suspension cultures.

  8. Highly Resolved Paleoclimatic Aerosol Records

    DEFF Research Database (Denmark)

    Kettner, Ernesto

    soluble aerosols can be analysed for concentration changes only, insoluble aeolian dust can reveal additional information on its atmospheric residence time via changes in the mean grain sizes. Volumes of particulate matter in ice cores are most reliably determined with Coulter counters, but since...... a Coulter counter performs measurements on discrete samples, it cannot be connected to a CFA system. Attenuation sensors, on the other hand, can be integrated into a CFA set-up, but are known to yield poor dust size records. The dilemma between high quality sizing and high depth resolution was found...... impossible to circumvent by employing a third detection technique - laser scattering. Reliable information on size changes, even relative ones, cannot be obtained using optical methods. It is therefore proposed to focus further efforts on electrical measurements, making use of the advancements made over...

  9. Evaluation of three methods for hemoglobin measurement in a blood donor setting

    Directory of Open Access Journals (Sweden)

    Jacob Rosenblit


    Full Text Available CONTEXT: The hemoglobin (Hb level is the most-used parameter for screening blood donors for the presence of anemia, one of the most-used methods for measuring Hb levels is based on photometric detection of cyanmetahemoglobin, as an alternative to this technology, HemoCue has developed a photometric method based on the determination of azide metahemoglobin. OBJECTIVE: To evaluate the performance of three methods for hemoglobin (Hb determination in a blood bank setting. DESIGN: Prospective study utilizing blood samples to compare methods for Hb determination. SETTING: Hemotherapy Service of the Hospital Israelita Albert Einstein, a private institution in the tertiary health care system. SAMPLE: Serial blood samples were collected from 259 individuals during the period from March to June 1996. MAIN MEASUREMENTS: Test performances and their comparisons were assessed by the analysis of coefficients of variation (CV, linear regression and mean differences. RESULTS: The CV for the three methods were: Coulter 0.68%, Cobas 0.82% and HemoCue 0.69%. There was no difference between the mean Hb determination for the three methods (p>0.05. The Coulter and Cobas methods showed the best agreement and the HemoCue method gave a lower Hb determination when compared to both the Coulter and Cobas methods. However, pairs of methods involving the HemoCue seem to have narrower limits of agreement (± 0.78 and ± 1.02 than the Coulter and Cobas combination (± 1.13. CONCLUSION: The three methods provide good agreement for hemoglobin determination.

  10. Merida Initiative: The Answer for Mexico? (United States)


    St ra te gy R es ea rc h Pr oj ec t MÉRIDA INITIATIVE: THE ANSWER FOR MEXICO ? BY LIEUTENANT COLONEL NANCY M. BOZZER United States Air...DATES COVERED (From - To) 4. TITLE AND SUBTITLE Mérida Initiative: The Answer for Mexico ? 5a. CONTRACT NUMBER 5b. GRANT NUMBER...NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Professor Daniel Coulter Department of National Security and Strategy Walter Bedell

  11. Stratigraphy and Glacial-Marine Sediments of the Amerasian Basin, Central Arctic Ocean. (United States)


    particles and conducting an electronic si/C analisis of the material that passed through the sieve A, using a I A 11 Coulter counter. I he (oulter...sized particles from suspension is similar for all of the deep-sea current with a velocity of 45 to 60 cm s would be required to cause sediments that...distribution of Arctic Ocean glacial-marine surface- relationship, the flow velocities required for erosion of incohesisc sediment types must be

  12. Analytical and clinical performance of thyroglobulin autoantibody assays in thyroid cancer follow-up. (United States)

    Katrangi, Waddah; Grebe, Stephan K G; Algeciras-Schimnich, Alicia


    While thyroglobulin autoantibodies (TgAb) can result in false low serum thyroglobulin (Tg) immunoassay (IA) measurements, they might also be indicators of disease persistence/recurrence. Hence, accurate TgAb measurement, in addition to Tg quantification, is crucial for thyroid cancer monitoring. We compared the analytical and clinical performance of four commonly used TgAb IAs. We measured Tg by mass spectrometry (Tg-MS) and by four pairs of Tg and TgAb IAs (Beckman, Roche, Siemens, Thermo) in 576 samples. Limit of quantitation (LOQ) and manufacturers' upper reference interval cut-off (URI) were used for comparisons. Clinical performance was assessed by receiving operator characteristics (ROC) curve analysis. Quantitative and qualitative agreement between TgAb-IAs was moderate with R2 of 0.20-0.70 and κ from 0.41-0.66 using LOQ and 0.47-0.71 using URI. In samples with TgAb interference, detection rates of TgAb were similar using LOQ and URI for Beckman, Siemens, and Thermo, but much lower for the Roche TgAb-IA when the URI was used. In TgAb positive cases, the ROC areas under the curve (AUC) for the TgAb-IAs were 0.59 (Beckman), 0.62 (Siemens), 0.59 (Roche), and 0.59 (Thermo), similar to ROC AUCs achieved with Tg. Combining Tg and TgAb measurements improved the ROC AUCs compared to Tg or TgAb alone. TgAb-IAs show significant qualitative and quantitative differences. For 2 of the 4 TgAb-IAs, using the LOQ improves the detection of interfering TgAbs. All assays showed suboptimal clinical performance when used as surrogate markers of disease, with modest improvements when Tg and TgAb were combined.

  13. Comparison of three different assays for measuring thyroglobulin and thyroglobulin antibodies in patients with chronic lymphocytic thyroiditis. (United States)

    Cheng, Xinqi; Yu, Songlin; Jin, Cheng; Han, Song; Hu, Yingying; Zhang, Kui; Liu, Huaicheng; Qiu, Ling


    To evaluate how thyroglobulin (Tg) and thyroglobulin antibody (TgAb) results compare among different chemiluminescence methods. Serum specimens from 153 patients with thyroiditis and 127 apparently healthy individuals were collected at the Peking Union Medical College Hospital from September 2014 to December 2014. TgAb and Tg levels were analyzed by using three different platforms manufactured by Roche, Beckman, and Siemens. The χ 2 test was performed to analyze the TgAb results from the different methods. Passing-Bablok regression equations and Bland-Altman plots were used to estimate the relationship and bias among the Tg results obtained with the different analyses. The overall coincidence rates for the TgAb results were 82.9% between the Beckman and Roche methods, 78.6% between the Siemens and Roche methods, and 81.1% between the Siemens and Beckman methods. The borderline positive coincidence rate among the different TgAb methods was very poor (9.1-24.2%). Of the 280 samples, the TgAb results for 26.4% of the samples differed among the three CLIA platforms. A good linear relationship among the Tg levels was shown in TgAb-negative subjects analyzed with the different CLIA systems. However, for TgAb-positive subjects, the linear relationship of the Tg levels measured with the Siemens system, compared with the other two systems, was poor. Bland-Altman plots demonstrated significant differences among the different Tg assays. Current Tg and TgAb methods are highly variable and cannot be used interchangeably. Copyright © 2017. Published by Elsevier Inc.

  14. Rapid detection of oleander poisoning using digoxin immunoassays: comparison of five assays. (United States)

    Dasgupta, Amitava; Datta, Pradip


    Oleander is an ornamental shrub that grows in the United States, Australia, India, Sri Lanka, China, and other parts of the world. All parts of the plant are poisonous because the presence of cardiac glycoside oleandrin. Despite its toxicity, oleander extract is used in folk medicines. Because of its structural similarity, oleandrin cross-reacts with the fluorescence polarization immunoassay (FPIA) for digoxin. We studied the potential of detecting oleandrin in serum using 5 common digoxin immunoassays (FPIA, MEIA, both from Abbott; Beckman digoxin assay on Synchron LX, Chemiluminescent assay, CLIA from Bayer Diagnostics) and a recently FDA-approved turbidimetric assay on the ADVIA 1650 analyzer (Bayer). Aliquots of drug-free and digoxin-like immunoreactive substances (DLIS)-free serum pools were supplemented with ethanol extract of oleander leaves or oleandrin (Sigma Chemicals) in amounts expected in vivo after severe overdose. We observed significant apparent digoxin concentration with FPIA, Beckman, and the new turbidimetric assay (1 mL drug-free serum supplemented with 5.0 microL of oleander extract: apparent digoxin 2.36 ng/mL by the FPIA, 0.32 ng/mL by the MEIA, 0.93 ng/mL by the Beckman, 0.82 ng/mL by the new turbidimetric assay). The CLIA showed no cross-reactivity. Similar observations were made when serum pools were supplemented with oleandrin. Because cross reactivity should be tested in the presence of the primary analyte, we supplemented serum pools prepared from patients receiving digoxin with oleander extract or oleandrin. The measured digoxin concentrations were falsely elevated with the FPIA, Beckman, and turbidimetric assays, the highest false elevation being observed with the FPIA. Surprisingly, apparent digoxin concentrations were falsely lowered when MEIA was used. Digibind neutralizes free apparent digoxin concentration in vitro in serum pools supplemented with oleander extract, and this effect can be measured by the FPIA. We conclude that

  15. Determination of average molecular weights on organic reactor coolants. I.- Freezing-point depression method for benzene solutions

    International Nuclear Information System (INIS)

    Carreira, M.


    As a working method for determination of changes in molecular mass that may occur by irradiation (pyrolytic-radiolytic decomposition) of polyphenyl reactor coolants, a cryoscopic technique has been developed which associated the basic simplicity of Beckman's method with some experimental refinements taken out of the equilibrium methods. A total of 18 runs were made on samples of napthalene, biphenyl, and the commercial mixtures OM-2 (Progil) and Santowax-R (Monsanto), with an average deviation from the theoretical molecular mass of 0.6%. (Author) 7 refs

  16. Solar energy in the United States

    International Nuclear Information System (INIS)

    Ochoa, D.; Slaoui, A.; Soler, R.; Bermudez, V.


    Written by a group of five French experts who visited several research centres, innovating companies and solar power stations in the United States, this report first proposes an overview of solar energy in the United States, indicating and commenting the respective shares of different renewable energies in the production, focusing on the photovoltaic energy production and its RD sector. The second part presents industrial and research activities in the solar sector, and more specifically photovoltaic technologies (silicon and thin layer technology) and solar concentrators (thermal solar concentrators, photovoltaic concentrators). The last chapter presents the academic research activities in different universities (California Tech Beckman Institute, Stanford, National Renewable Energy Laboratory, Colorado School of Mines)

  17. Determination of average molecular weights on organic reactor coolants. I.- Freezing-point depression method for benzene solutions; Determinaciond e masas moleculares medias en refrigerantes nucleares organicos. I.- Crioscopia de disolucion bencenicas

    Energy Technology Data Exchange (ETDEWEB)

    Carreira, M.


    As a working method for determination of changes in molecular mass that may occur by irradiation (pyrolytic-radiolytic decomposition) of polyphenyl reactor coolants, a cryoscopic technique has been developed which associated the basic simplicity of Beckman's method with some experimental refinements taken out of the equilibrium methods. A total of 18 runs were made on samples of napthalene, biphenyl, and the commercial mixtures OM-2 (Progil) and Santowax-R (Monsanto), with an average deviation from the theoretical molecular mass of 0.6%. (Author) 7 refs.

  18. Determination of average molecular weights on organic reactor coolants. II.-Freezing point depression method for diphenyl-ether solutions; Determinacion de masas moleculares medias en refrigerantes nucleares organicos. II.- Crioscopia de disoluciones en eter difenilico

    Energy Technology Data Exchange (ETDEWEB)

    Carreira, M.


    In order to reduce limitations of solubility, the cryoscopic method developed for benzene solutions of polyphenyl mixtures has been extended to diphenyl-ether solutions by introducing some modifications imposed by the physico-chemical properties of this solvent. The Nernsto theory of Beckman's method has been revised, taking into account the heat-transfer characteristics of the system, and the results of that analysis have been used to fix upon the design parameters of a cryoscopic apparatus for measurements on diphenyl-ether solutions. (Author) 9 refs.

  19. Studies of microparticles in patients with the antiphospholipid syndrome (APS). (United States)

    Vikerfors, A; Mobarrez, F; Bremme, K; Holmström, M; Ågren, A; Eelde, A; Bruzelius, M; Antovic, A; Wallén, H; Svenungsson, E


    To study circulating platelet, monocyte and endothelial microparticles (PMPs, MMPs and EMPs) in patients with antiphospholipid syndrome (APS) in comparison with healthy controls. Fifty-two patients with APS and 52 healthy controls were investigated. MPs were measured on a flow cytometer (Beckman Gallios) and defined as particles sized APS patients versus controls (p APS patients. We observed a high number of EMPs expressing TF in APS patients. The numbers of MMPs and total EMPs were also higher as compared with healthy controls but in contrast to previous reports, the number of PMPs did not differ between groups.

  20. Quantitative Assessment of Graded Burn Wounds in a Porcine Model using Spatial Frequency Domain Imaging (SFDI) and Laser Speckle Imaging (LSI) (United States)


    Adrien Ponticorvo, 1,4 David M. Burmeister, 3,4 Bruce Yang, 1 Bernard Choi, 1,2 Robert J. Christy, 3 and Anthony J. Durkin 1,* 1Beckman Laser...comparison of laser Doppler imaging and videomicroscopy,” Burns 33(7), 833–842 (2007). 4. A. M. Watts, M. P. Tyler, M. E. Perry, A. H. Roberts , and D...157 (2010). 45. P. Ganapathy, T. Tamminedi, Y. Qin, L. Nanney, N. Cardwell, A. Pollins , K. Sexton, and J. Yadegar, “Dual- imaging system for burn

  1. Photophysics of Aqueous Pt(CN)4(2-). (United States)


    concentration dependence of the more intense absorption bands, however, it was necessary to use a micrometer , variable path-length cell (Beckman-Research...and Industrial Instruments Co., Ltd., England, model BC-14). Pathlength settings would be read to 0.0005 cm directly and to 5xO 5 cm with the vernier ...measurements using t %e micrometer cell are shown in Fig. 1. The features of interest are t ,i lack of concentration dependence of the peak at 280 nm and the

  2. A comparison of analytical methods for detection of [14C]trichloro acetic acid-derived radioactivity in needles and branches of spruce (Picea sp.)

    International Nuclear Information System (INIS)

    Kretzschmar, M.; Matucha, M.; Uhlirova, H.


    The branches (wood and needles) of spruces of varying age treated with [ 14 C]trichloro acetic acid (3.7 GBq/mmol) were studied, using the following methods: Qualitative: - Conventional macroautoradiography with X-ray film and histological classification. Quantitative: - 14 C combustion analysis with the sample oxidizer A 307 (Canberra/Packard) followed by measurement of radioactivity using the LS counter 6000 (Beckman Instrumentts); - digital autoradiography with the Digital Autoradiograph LB 286 (Berthold GmbH); -digital autoradiography with the Bio-imaging Analyzer BAS 2000 (Fuji Film Co.). (orig.)

  3. Determination of average molecular weights on organic reactor coolants. II.-Freezing point depression method for diphenyl-ether solutions

    International Nuclear Information System (INIS)

    Carreira, M.


    In order to reduce limitations of solubility, the cryoscopic method developed for benzene solutions of polyphenyl mixtures has been extended to diphenyl-ether solutions by introducing some modifications imposed by the physico-chemical properties of this solvent. The Nernsto theory of Beckman's method has been revised, taking into account the heat-transfer characteristics of the system, and the results of that analysis have been used to fix upon the design parameters of a cryoscopic apparatus for measurements on diphenyl-ether solutions. (Author) 9 refs

  4. Extraction and analysis of Ursolic Acid from Spica Prunellae


    Kai-quan LI; Wen-feng YE; Dao-an XIAO


    Objective: To study the extract technology of Ursolic Acid from Spica Prunellae,and to build the method of determining its content. Methods: Adopting the method of ethanol extract and agglutination separate to isolate Ursolic Acid.The content of Ursolic Acid in sample is determined by HPLC with Methanol-H2O-Glacial acetic acid-Triethylamine (83:17:0.04:0.02) as mobile phase at a flow rate of 1.0ml/min,and detection wavelength at 210nm. Beckman ODS 150mm×4.6mm column is used at 25℃. Results: T...

  5. Biomedical foundation supports technology aimed at destroying cancer cells


    Nystrom, Lynn A.


    A new technology, using electric pulses to destroy cancer tissue and named by NASA Tech Briefs as one of seven key technological breakthroughs of 2007, is receiving additional support aimed at moving the procedure to the marketplace. One of its lead developers, Rafael V. Davalos, a faculty member of the Virginia Tech-Wake Forest University School of Biomedical Engineering and Sciences (SBES), received a $240,000 grant from the Wallace H. Coulter Foundation and $25,000 from the Wake Forest Com...

  6. Proxies and measurement techinques for mineral dust in antarctic ice cores

    DEFF Research Database (Denmark)

    Ruth..[], Urs; Bigler, Matthias


    To improve quantitative interpretation of ice core aeolian dust records, a systematic methodological comparison was made. This involved methods for water-insoluble particle counting (Coulter counter and laser-sensing particle detector), soluble ion analysis (ion chromatography and continuous flow...... (EDML) cores were used. All methods correlate very well among each other, but the ratios of glacial age to Holocene concentrations, which are typically a factor 100, differ between the methods by up to a factor of 2 with insoluble particles showing the largest variability. The recovery of ICP...

  7. Glaciological and chemical studies on ice cores from Hans Tausen ice cap, Greenland

    DEFF Research Database (Denmark)

    Clausen, H.B.; Stampe, Mia; Hammer, C.U.


    The paper presents studies of various chemical and isotopical parameters from ice cores drilled in the northernmost located ice cap, Hans Tausen Iskappe, Pearyland, Greenland (HT). The 346 m main core (MC95) was drilled to bedrock in 1995 as well as a 35 m shallow core (SC95). A 60 m shallow core...... are selected for an analysis of dust and water soluble chemical components, including F-, CH3SO2-, Cl-, NO3-, SO42-, Na+, NH4+, K+, Mg2+ and Ca2+. Coulter counter technique was used for the dust measurements and the chemical analysis were carried out by ion chromatography....

  8. Crystal Chemical Substitutions of YBa2Cu3O7-d to Enhance Flux Pinning (Postprint) (United States)


    1.015 0.000 0210 J. at0.1 T [98] 0.200 0.350 0.400 0.680 0.700 0.290 1.000 0.190 y 1.019 v, .• oy . PLDfilms 1.027 0.000 0.300 [19] 0.200 2.800...Physica C 314 (1999) 55-68. L. Cao, J. Zegenhagen, Phys. Stat. Sol. 215 (1999) 587-589. C. Kwon , L.R. Kinder, Y. Gim, Y. Fan, J.Y. Coulter, M.P

  9. Analysis of images of acute human and animal leukaemia

    International Nuclear Information System (INIS)

    Feinermann, Emmanuel


    This research thesis first proposes a review of the development of stereology: historical backgrounds, basic principles. It discusses the choices regarding instrumentation: Coulter counter (principle and theory), quantitative analysis of particles, image analyser (optical microscope, epidiascope, scanners, detection, electronic pencil, computers, programming and data processing system), and stereo-logical parameters. The author then reports the stereo-logical study of acute human leukaemia: definition, classification, determination of spherical particle size distribution, lympho-blast size distributions. He reports the comparative study of rat L 5222 leukaemia and Brown Norway rat acute myelocytic leukaemia, and discusses their relationship with acute human leukaemia

  10. Interaction of erythrocytes and hexavalent uranium compounds -an autoanalytical study

    International Nuclear Information System (INIS)

    Stuart, W.I.; Shying, M.E.


    An automated analytical system was devised to measure the kinetics of hemolysis by uranyl compounds. Accurate plots of percentage hemolysis v. time were obtained; these, together with the corresponding differential curves, show that hemolysis of plasma-free erythrocytes is a two-stage process. The first stage of hemolysis is particularly affected by pH and anion content of uranyl solutions, and also by incubation of cell suspensions at 37 deg. before mixing with lysing solution. Complementary studies involving Coulter counting and microscopic observation established the general pattern of hemolysis and showed that cell agglutination is a prominent feature of the interaction of cells with uranyl solutions

  11. Determinação espectrofotométrica de cálcio em solos Spectrophotometric determination of calcium in soils

    Directory of Open Access Journals (Sweden)

    Fernando A. Soares Coelho


    Full Text Available É apresentado estudo da determinação de cálcio em extratos de solo por espectrofotometria de chama, utilizando o espectrofotômetro Beckman, modelo DU. A composição da chama, as pressões dos gases, as interferências e os métodos para eliminá-las foram estudados. Verificou-se ser imprescindível a eliminação das interferências causadas pelos íons de alumínio, ferro e fosfato nas concentrações que normalmente ocorrem nos solos. A utilização de EDTA a pH 9 apresentou resultados satisfatórios na eliminação dessas interferências quando em concentração 0,01 M na solução em que foi determinado o cálcio.A study is presented on the determination of calcium in soil extracts by flame photometry, using a Beckman Model DU spectrophotometer. Flame composition, gas pressure, interferences and their elimination were studied. Results showed it to be necessary to eliminate interferences due to aluminum, iron and phosphate ions in amounts normally present in soils. Enough EDTA solution at pH 9.0 was added to the soil extract until a concentration of 0.01 M in EDTA was reached.

  12. Assay of cerebrospinal fluid protein: a rate biuret method evaluated. (United States)

    Finley, P R; Williams, R J


    We evaluated a rate colorimetric method (Beckman) for measuring total protein in cerebrospinal fluid. The automated instrument we used was Beckman's ASTRA TM. A 100-microL sample of spinal fluid is introduced into the biuret reagent in the reaction cell and the increase in absorbance at 545 nm is monitored for 20.5 s. Solid-state circuits determine the rate of alkaline biuret-protein chelate formation, which is directly proportional to the total protein concentration in the sample. The linear range of measurement is 120 to 7500 mg/L. Day-to-day precision (CV) over the range of 150 to 1200 mg/L ranged from 15.2 to 2.3%. The method was unaffected by radical alteration of the albumin/globulin ratio, but there is a positive interference in the presence of hemoglobin, a suppression in the presence of bilirubin, and no effect by xanthochromia. The method is precise, accurate, rapid, and convenient. The method was compared with the trichloroacetic acid method as performed on the Du Pont aca III, giving a correlation coefficient (r2) of 0.9693. The method is precise, accurate, rapid, and convenient.

  13. Evaluation of a capillary zone electrophoresis system versus a conventional agarose gel system for routine serum protein separation and monoclonal component typing. (United States)

    Roudiere, L; Boularan, A M; Bonardet, A; Vallat, C; Cristol, J P; Dupuy, A M


    Capillary zone electrophoresis of serum proteins is increasingly gaining impact in clinical laboratories. During 2003, we compared the fully automated capillary electrophoresis (CE) system from Beckman (Paragon CZE 2000) with the method agarose gel electrophoresis Sebia (Hydrasis-Hyris, AGE). This new study focused on the evaluation of analytical performance and a comparison including 115 fresh routine samples (group A) and a series of 97 frozen pathologic sera with suspicion of monoclonal protein (group B). Coefficients of variation (CVs %) for the five classical protein fractions have been reported to be consistenly serum samples (group B), there were 90 in which we detected a monoclonal protein by immunofixation (IF) (immunosubtraction (IS) was not used). AGE and Paragon 2000 failed to detect 7 and 12 monoclonal proteins, respectively, leading to a concordance to 92% for AGE and 87% for Paragon 2000 for identifying electrophoretic abnormalities in this group. Beta-globulin abnormalities and M paraprotein were well detected with Paragon 2000. Only 81% (21 vs 26) of the gammopathies were immunotyped with IS by two readers blinded to the IF immunotype. The Paragon 2000 is a reliable alternative to conventional agarose gel electrophoresis combining the advantages of full automation (rapidity, ease of use and cost) with high analytical performance. Qualified interpretation of results requires an adaptation period which could further improve concordance between the methods. Recently, this CE system has been improved by the manufacturer (Beckman) concerning the migration buffer and detection of beta-globulin abnormalities.

  14. Trueness investigation of routine creatinine assays on nine homogeneous systems in Beijing demonstrates an encouraging outcome that meets clinical requirements. (United States)

    Liu, Yan; Xu, Guo-bin


    Serum creatinine (Scr) measurement plays a key role in glomerular filtration rate estimation (eGFR), chronic kidney disease (CKD) diagnosis as well as CKD treatment. However, the test results of Scr from different laboratories vary significantly. In order to get comparable results, the European in vitro diagnostic (IVD) directive requires traceability to reference methods and materials. The purpose of this study was to verify the effect of traceability implementation by investigating the trueness of creatinine measurement on nine homogenous systems in Beijing. Commutable frozen human serum reference material, National Institute of Standards & Technology (NIST) Standard Reference Material (SRM) 967, was used to verify the trueness of Scr measurement results from nine homogeneous analytical systems of seven companies which are the most widely used systems in Beijing's third-grade hospitals. The methods referred to the Jaffe's and Enzymatic methods. from nine routine measurement systems were assessed using two criteria: biological variability and Clinical Laboratory Improvement Amendments' 88 (CLIA' 88). We simulated a series of broken lines representing the limits of SD and bias that would produce a relative increase (or decrease) of 10% and 20% in the measurement error when estimating GFR (MEeGFR) using the isotope dilution mass spectrometry (IDMS)-traceable Modification of Diet in Renal Disease (MDRD) Study equation. of the College of American Pathologists (CAP) 2008-B LN24 Survey were compared with our investigation results. Compared with the total error criteria of biological variability, Ortho (traceable to IDMS) met the minimum acceptable criteria; Roche (Jaffe), Roche (Enzymatic), Shino and Daiichi met the desirable criteria at level I. At level II, Ortho (traceable to gas chromatography/isotope dilution mass spectrometry, GC/IDMS), Dade Behring and Beckman (traceable to rate Jaffe) met the minimum acceptable criteria; Roche (Enzymatic) met the optimum

  15. Is automated platelet counting still a problem in thrombocytopenic blood?

    Directory of Open Access Journals (Sweden)

    Raimundo Antônio Gomes Oliveira

    Full Text Available CONTEXT: Reliable platelet counting is crucial for indicating prophylactic platelet transfusion in thrombocytopenic patients. OBJECTIVE: To evaluate the precision and accuracy of platelet counting for thrombocytopenic patients, using four different automated counters in comparison with the Brecher & Cronkite reference method recommended by the International Committee for Standardization in Hematology (ICSH. TYPE OF STUDY: Automated platelet counting assessment in thrombocytopenic patients. SETTING: Hematology Laboratory, Hospital do Servidor Público Estadual de São Paulo, and the Hematology Division of Instituto Adolfo Lutz, São Paulo, SP, Brazil. MAIN MEASUREMENTS: Brecher & Cronkite reference method and four different automated platelet counters. PARTICIPANTS: 43 thrombocytopenic patients with platelet counts of less than 30,000/µl RESULTS: The ADVIA-120 (Bayer, Coulter STKS, H1 System (Technicom-Bayer and Coulter T-890 automatic instruments presented great precision and accuracy in relation to laboratory thrombocytopenic samples obtained by diluting blood from normal donors. However, when thrombocytopenic patients were investigated, all the counters except ADVIA (which is based on volume and refraction index showed low accuracy when compared to the Brecher & Cronkite reference method (ICSH. The ADVIA counter showed high correlation (r = 0.947. However, all counters showed flags in thrombocytopenic samples. CONCLUSION: The Brecher & Cronkite reference method should always be indicated in thrombocytopenic patients for platelet counts below 30,000 plt /µl obtained in one dimensional counters.

  16. The efficiency of new leukocyte removal filters. CF-1 and CF-2. (United States)

    Endo, Y; Tani, T; Ebira, Y; Araki, H; Yokota, T; Tsutamoto, Y; Abe, H; Numa, K; Matsuda, K; Aoki, H


    Two new leukocyte removal filters, Nipro CF-1 and CF-2 (Nipo Medical Industries, Osaka, Japan), were evaluated. These non-woven polyester filters, which are gravity flow devices that require no priming and no rinsing after use, were developed for preparing 400 ml of whole blood or red cell concentrates from 400 ml of whole blood. A flow cytometric technique was developed to measure extremely low white blood cell (WBC) counts. To evaluate the efficiency of these filters, leukocyte counts were measured by three techniques: 1) electronic, 2) visual, and 3) flow cytometry. Flow cytometric counting was done using a Coulter EPICS-C cytometer (Coulter Corp., Hialeah, FL). Nipro CF-1 removed 99.97 +/- 0.01% (mean +/- SD, n = 14) of leukocytes measured by flow cytometry, and CF-1 recovered 90.7 +/- 4.47% (n = 21) of red blood cells. After filtration through CF-2, more than a 6 log10 (> 99.9999%) depletion of WBCs was detected in six samples, a 6 log10 (99.9999%) depletion of WBCs was detected in two samples, a 5 log10 (99.999%) depletion was detected in five samples, and a 4 log10 (99.99%) depletion was detected in one sample. CF-1, in which size and priming volume was smaller than other commercial leukocyte removal filters, accomplished a 3 log10 reduction in WBC count, compared with other commercial filters. CF-2 achieved a 4-6 log10 depletion of WBCs assayed by flow cytometry.

  17. Applicability of a prototype for determination of absorbed dose using brachytherapy equipment with Ir-192 sources; Aplicabilidade de um prototipo para determinacao da dose absorvida utilizando equipamentos de braquiterapia com fontes de IR-192

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Vivianne Lucia Bormann; Almeida, Mayara Gabriella Oliveira de; Vieira, Rafaela Etelvina de Amorim; Silva, Waldecy Ananias da; Nascimento, Rizia Keila, E-mail:, E-mail:, E-mail:, E-mail:, E-mail: [Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil)


    This work aims at the development and improvement of a device to perform the absolute dosimetry sources of Ir-192 using the Fricke solution contained in a flask. The Fricke solution used was prepared using amounts of ferrous ammonium sulfate, sodium chloride and sulfuric acid, diluted with water tri distilled pre-established in the literature. The spectrophotometer used was a UV-VIS spectrophotometer (Beckman DU-640 Counter) for measuring the optical density at wavelength 304 nm. The calculation for determining the radial dose takes into account the radial distance and the angle formed with the transverse axis of the source. As the results obtained can be seen that the states of Pernambuco, Ceara, Paraiba e Piaui are in accordance with the recommendations of international standards of the International Atomic Energy Agency (IAEA), which considers not acceptable a difference greater than 5% of prescribed dose and measured dose.

  18. Discussing epigenetics in Southern California: a report from the International Symposium on Epigenetic Control and Cellular Plasticity, UCI, December 15-16, 2011. (United States)

    Rattner, Barbara P


    With the goal of discussing how epigenetic control and chromatin remodeling contribute to the various processes that lead to cellular plasticity and disease, this symposium marks the collaboration between the Institut National de la Santé et de la Recherche Médicale (INSERM) in France and the University of California, Irvine (UCI). Organized by Paolo Sassone-Corsi (UCI) and held at the Beckman Center of the National Academy of Sciences at the UCI campus December 15-16, 2011, this was the first of a series of international conferences on epigenetics dedicated to the scientific community in Southern California. The meeting also served as the official kick off for the newly formed Center for Epigenetics and Metabolism at the School of Medicine, UCI (

  19. Discussing epigenetics in Southern California (United States)


    With the goal of discussing how epigenetic control and chromatin remodeling contribute to the various processes that lead to cellular plasticity and disease, this symposium marks the collaboration between the Institut National de la Santé et de la Recherche Médicale (INSERM) in France and the University of California, Irvine (UCI). Organized by Paolo Sassone-Corsi (UCI) and held at the Beckman Center of the National Academy of Sciences at the UCI campus December 15–16, 2011, this was the first of a series of international conferences on epigenetics dedicated to the scientific community in Southern California. The meeting also served as the official kick off for the newly formed Center for Epigenetics and Metabolism at the School of Medicine, UCI ( PMID:22414797

  20. Secoisolariciresinol Diglucoside (SDG) Isolated from Flaxseed, an Alternative to ACE Inhibitors in the Treatment of Hypertension. (United States)

    Prasad, Kailash


    Secoisolariciresionol diglucoside (SDG) is a plant lignan isolated from flaxseed and is phytoestrogen. SDG is a potent and long-acting hypotensive agent. Plant phytoestrogens have inhibitory effects on angiotensin-converting enzyme (ACE). The hypotensive effects of SDG, a phytoestrogen, may be mediated through inhibition of ACE. The objective of this study was to investigate if SDG-induced hypotension is mediated through inhibition of ACE. The Sprague Dawley male rats were anesthetized and trachea was cannulated. The right jugular vein was cannulated to administer the drug and the carotid artery was cannulated to record arterial pressures using PIOEZ-1 miniature model transducer (Becton, Dickinson and Company, Franklin Lakes, NJ) and Beckman dynograph (Beckman Instruments, Inc., Schiller Park, IL). The effects of angiotensin I (0.2 µg/kg, intravenously [IV]) in the absence and presence of SDG (10 mg/kg, IV), and SDG alone on systolic, diastolic, and mean arterial pressures were measured before and after 15, 30, and 60 minutes of drug administration. SDG decreased the systolic, diastolic, and mean arterial pressure by 37, 47, and 43%, respectively, at 15 minutes and 18.8, 21.2, and 20.3%, respectively, at 60 minutes. Angiotensin I increased the arterial pressure. SDG decreased angiotensin I-induced rise in the systolic, diastolic, and mean arterial pressures by 60, 58, and 51%, respectively, at 15 minutes and 48, 46, and 30%, respectively, at 60 minutes. The data suggest that SDG reduced the angiotensin I-induced rise in the arterial pressures and hence SDG is a potent ACE inhibitor.

  1. Outcome prediction for prostate cancer detection rate with artificial neural network (ANN) in daily routine. (United States)

    Ecke, Thorsten H; Bartel, Peter; Hallmann, Steffen; Koch, Stefan; Ruttloff, Jürgen; Cammann, Henning; Lein, Michael; Schrader, Mark; Miller, Kurt; Stephan, Carsten


    We evaluated the use of the artificial neural network (ANN) program "ProstataClass" of the Department of Urology and the Institute of Medical Informatics at the Charité-Universitätsmedizin Berlin in daily routine to increase prostate cancer (CaP) detection rate and to reduce unnecessary biopsies. From May 2005 to April 2007, a total of 204 patients were included in the study. The Beckman Access PSA assay was used, and pretreatment prostate specific antigen (PSA) was measured prior to digital rectal examination (DRE) and 12 core systematic transrectal ultrasound (TRUS) guided biopsies. The individual ANN predictions were generated with the use of the ANN application for the Beckman Access PSA and free PSA assays, which relies on age, PSA, percent free prostate specific antigen (%fPSA), prostate volume, and DRE. Diagnostic validity of total prostate specific antigen (tPSA), %fPSA, and the ANN was evaluated by ROC curve analysis. PSA and %fPSA ranged from 4.01 to 9.91 ng/ml (median: 6.65) and 5% to 48% (median: 15%), respectively. Of all men, 46 (22.5%) demonstrated suspicious DRE findings. Total prostate volume ranged from 7.1 to 119.2 cc (median: 35). Overall, 71 (34.8%) CaP were detected. Of men with suspicious DRE, 28 (60.9%) had CaP on initial biopsy. The ANN was 78% accurate in the original report. The AUC of ROC curve analysis was 0.51 for PSA, 0.66 for %PSA, and 0.72 for the ANN-Output, respectively. Our results in this independent cohort show that ANN is a very helpful parameter in daily routine to increase the CaP detection rate and reduce unnecessary biopsies. Copyright © 2012 Elsevier Inc. All rights reserved.

  2. Comparison of method-related reference intervals for thyroid hormones: studies from a prospective reference population and a literature review. (United States)

    Barth, Julian H; Luvai, Ahai; Jassam, Nuthar; Mbagaya, Wycliffe; Kilpatrick, Eric S; Narayanan, Deepa; Spoors, Shirley


    Introduction Reference intervals are dependent on the reference population, the analytical methods and the way the data are handled statistically. Individual method-related differences have been studied but the comparative differences in reference intervals have not. Methods We studied a reference population of healthy adult subjects and measured free thyroxine and thyroid-stimulating hormone by the four most commonly used analytical platforms used in the UK. Subjects were excluded if they were > 65 years or had positive thyroid peroxidase antibodies. We also performed a systematic literature review of thyroid hormone reference interval studies in non-pregnant adults. Results In total, 303 subjects were recruited and 42 excluded. The central 95th centile values for thyroid-stimulating hormone (mIU/L) were Abbott Architect (0.51-3.67); Beckman Unicel DxI (0.57-3.60); Roche Cobas (0.60-4.31) and Siemens Advia Centaur XP (0.63-4.29). The 95th centile values for thyroxine (pmol/L) were Abbott Architect (10.6-15.5); Beckman Unicel DxI (7.9-13.0); Roche Cobas (12.5-19.6) and Siemens Advia Centaur XP (11.8-19.0). We identified 55 papers describing thyroid reference intervals in male and non-pregnant female adults. The values for upper and lower reference intervals by manufacturer varied but were not significantly different for thyroid-stimulating hormone but were for thyroxine. Discussion Our study demonstrates clearly that there are marked variations in the reference intervals for thyroid hormones between analytical platforms. There is an urgent need for standardization of thyroid hormone assays to permit transferability of results. Until then, guidelines will need to reflect this method-related difference.

  3. A plate reader-based method for cell water permeability measurement

    DEFF Research Database (Denmark)

    Fenton, Robert A.; Moeller, H B; Nielsen, S


    Cell volume and water permeability measurements in cultured mammalian cells are typically conducted under a light microscope. Many of the employed approaches are time consuming and not applicable to a study of confluent epithelial cell monolayers. We present here an adaptation of a calcein......-mannitol concentrations. Similarly, according average cell volumes have been measured in suspension in a Coulter counter (particle-sizing device). Based on these measurements, we have derived an equation that facilitates the modeling of cell volume changes based on fluorescence intensity changes. We have utilized...... the method to study the role of a carboxyl-terminus aquaporin (AQP)-2 phosphorylation site, which is known to affect AQP2 membrane trafficking, in heterologous type I Madin-Darby canine kidney cells. We find that water permeability in cells expressing phosphorylation site mutants was in the following order...

  4. Crop establishment of Silphium perfoliatum by precision seeding

    Directory of Open Access Journals (Sweden)

    Andreas Schäfer


    Full Text Available Silphium perfoliatum, a perennial composite from North America, has been found as a promising plant species during the search for alternative biogas substrates. Until now Silphium perfoliatum has been established by the time- and cost-intensive transplanting method. The precision sowing of Silphium perfoliatum reduces the process costs significantly. However, the bulky seed with mildness germination power complicates a well distributed and adequate plant establishment. In field tests a modified precision seeder was used for sowing Silphium perfoliatum which is primarily used for maize sowing.The hole diameter of the singling disc, the sowing coulter and the roller were modified. In the field tests the modified machinery proved an enhanced and uniform field emergence of Silphium perfoliatum.

  5. Investigations of heavy ion tracks in polyethylene naphthalate films

    CERN Document Server

    Starosta, W; Sartowska, B; Buczkowski, M


    The heavy ion beam (with fluence 3x10 sup 8 ion/cm sup 2) from a cyclotron has been used for irradiation of thin polyethylene naphthalate (PEN) films. Latent tracks in these polymeric films have been sensitized by UV radiation and then chemically etched in NaOH solution. The etching process parameters have been controlled by the electroconductivity method. After etching, parameters of samples have been examined by SEM and bubble point methods (Coulter[reg] Porometer II instrument). Results have shown good quality of PEN track membranes with pore sizes in the range: 0.1 - 0.5 mu m. The described procedure is known for thin polyethylene terephthalate (PET) films. Taking into consideration that PEN films have got better mechanical, thermal, gas barrier as well as better chemical resistance properties in comparison with PET films, the possibility of application of such membranes is much wider.

  6. Influence of suspension osmolarity and erythrocyte volume on cell deformability

    Energy Technology Data Exchange (ETDEWEB)

    Feo, C. (Institut de Pathologie Cellulaire, INSERM, 94 - Kremlin-Bicetre (France)); Phillips, W.M. (School of Mechanical Engineering, Purdue University, West Lafayette, IN (USA))


    Erythrocytes were suspended in dextran solutions of phosphate buffered saline with solution osmolarities from 400 to 20 mosM/kg. The dilute suspensions were subjected to linear shear and their deformation determined by laser diffractometry (Ektacytometer). Cell volumes were measured using a Coulter counter following fixation in glutaraldehyde to eliminate the influence of deformability on the volume measurement. Minimum deformability generally agreed with the maximum cellular volume produced by hypotonic solutions. However, reduced deformability was observed for both hyperosmotic and hypoosmotic conditions. The oncotic effect of the dextran delayed hemolysis to surprisingly low values of solution osmolarity. In contrast with the usual osmotic fragility results, in the hypotonic dextran solutions there was no evidence of hemoglobin release. At low shear stresses, deformability was found to be enhanced by reducing intracellular viscosity (via osmotic water transport into the cell). However, the maximum cellular deformation obtained at high shear stress was always less than for the normal discocyte at normal osmolarities.

  7. Survival and activity of Streptococcus faecalis and Escherichia coli in tropical freshwater

    Energy Technology Data Exchange (ETDEWEB)

    Muniz, I.; Jimenez, L.; Toranzos, G.A.; Hazen, T.C. [Univ. of Puerto Rico, Rio Piedras (Puerto Rico)


    The survival of Streptococcus facecalis and Escherichia coli was studied in situ in a tropical rain forest watershed using membrane diffusion chambers. Densities were determined by acridine orange direct count and Coulter Counter. Population activity was determined by microautoradiography, cell respiration, and by nucleic acid composition. Densities of S. facecalis and E. coli decreased less than 1 log unit after 105 h as measured by direct count methods. Activity as measured by respiration, acridine orange activity, and microautoradiography indicated that both bacteria remained moderately active during the entire study. After 12 h, E. coli was more active than S. faecalis as measured by nucleic acid composition. E. coli and S. faecalis survived and remained active for more than 5 days. Consequently, both would seem to be unsuitable as indicators of recent fecal contamination in tropical waters.

  8. Reconnaissance surficial geologic map of the Taylor Mountains quadrangle, southwestern Alaska (United States)

    Wilson, Frederic H.


    This map and accompanying digital files are the result of the interpretation of aerial photographs from the 1950s as well as more modern imagery. The area, long considered a part of Alaska that was largely not glaciated (see Karlstrom, 1964; Coulter and others, 1965; or Péwé, 1975), actually has a long history reflecting local and more distant glaciations. An unpublished photogeologic map of the Taylor Mountains quadrangle from the 1950s by J.N. Platt Jr. was useful in the construction of this map. Limited new field mapping in the area was conducted as part of a mapping project in the Dillingham quadrangle to the south (Wilson and others, 2003); however, extensive aerial photograph interpretation represents the bulk of the mapping effort. The accompanying digital files show the sources for each line and geologic unit shown on the map.

  9. Survival and activity of Streptococcus faecalis and escherichia coli in tropical freshwater

    Energy Technology Data Exchange (ETDEWEB)

    Muniz, I; Toranzos, G.A. (Univ. of Puerto Rico, Rio Piedras (Puerto Rico)); Jimenez, L.; Hazen, T.C.


    The survival of Streptococcus faecalis and Escherichia coli was studied in situ in a tropical rain forest watershed using membrane diffusion chambers. Densities were determined by acridine orange direct count and Coulter Counter. Population activity was determined by microautoradiography, cell respiration, and by nucleic acid composition. Densities of S. faecalis and E. coli decreased less than 1 log unit after 105 hours as measured by direct count methods. Activity as measured by respiration, acridine orange activity, and microautoradiography indicated that both bacteria remained moderately active during the entire study. After 12 hours, E. coli was more active than S. faecalis as measured by nucleic acid composition. In this tropical rain forest watershed, E. coli and S. faecalis survived and remained active for more than 5 days; consequently, both would seem to be unsuitable as indicators of recent fecal contamination in tropical waters.

  10. El liderazgo docente y su relación con el estilo gerencial de los pasantes de Comunicación Social de la Universidad del Zulia

    Directory of Open Access Journals (Sweden)

    Rosario Fonseca


    Full Text Available Se propuso determinar la relación entre el liderazgo docente y el estilo gerencial de los estudiantes de Comunicación Social, mención Periodismo Impreso de LUZ, de acuerdo a la clasificación de Robbins, Stephen y Coulter (1999, y Guédez (1996 y siguiendo una metodología descriptiva-correlacional. Se aplicó un cuestionario para docentes y estudiantes de los últimos semestres. Los resultados demuestran que existe relación entre el liderazgo de los docentes, en su mayoría de tipo Democrático-Situacional, y el estilo gerencial de los estudiantes, predominó el estilo Estratégico y Situacional.

  11. Red blood cell sorting with a multi-bed microfabricated filter

    International Nuclear Information System (INIS)

    Layton, Bradley E; Lynch, Bernard; Jamieson, Brian; Peter, Thomas


    A microfabricated fluidic chip for sorting red blood cells (RBCs) by size has been designed, fabricated and tested. The performance of the chip has been compared against a flow cytometer using samples from identical populations of cells, and statistically significant (p < 0.0005) differences in the measured cell size distributions were observed. The measurement paradigm reported here differs from previously demonstrated devices such as microfabricated Coulter counters or flow cytometers, in that the analysis is inherently parallel and is thus suitable for high throughput, point-of-care analysis. This study is empirical and semi-quantitative. However, important features of RBC trapping are characterized and indications for improved device design are described. (paper)

  12. Ultra-Fast Low Concentration Detection of Candida Pathogens Utilizing High Resolution Micropore Chips

    Directory of Open Access Journals (Sweden)


    Full Text Available Although Candida species are the fourth most common cause of nosocomial blood stream infections in the United States, early diagnostic tools for invasive candidemia are lacking. Due to an increasing rate of candidemia, a new screening system is needed to detect the Candida species in a timely manner. Here we describe a novel method of detection using a solid-state micro-scale pore similar to the operational principles of a Coulter counter. With a steady electrolyte current flowing through the pore, measurements are taken of changes in the current corresponding to the shape of individual yeasts as they translocate or travel through the pore. The direct ultra-fast low concentration electrical addressing of C. albicans has established criteria for distinguishing individual yeast based on their structural properties, which may reduce the currently used methods’ complexity for both identification and quantification capabilities in mixed blood samples

  13. Visible and subvisible particles in the BCG immunotherapeutic product Immucyst®. (United States)

    Kirkitadze, Marina; Remi, Elena; Bhandal, Kamajit; Carpick, Bruce


    Bacille Calmette-Guerin, BCG, is a live attenuated bovine tubercle bacillus used for the treatment of non-muscle invasive bladder cancer. In this study, an Electrical Sensing Zone (ESZ) method was developed to measure the particle count and the size of BCG immunotherapeutic (BCG IT), or ImmuCyst® product using a Coulter Counter Multisizer 4® instrument. The focus of this study was to establish a baseline for reconstituted lyophilized BCG IT product using visible and sub-visible particle concentration and size distribution as reportable values. ESZ method was used to assess manufacturing process consistency using 20 production scale lots of BCG IT product. The results demonstrated that ESZ can be used to accumulate product and process knowledge of BCG IT.

  14. Ultra-sensitive flow measurement in individual nanopores through pressure--driven particle translocation. (United States)

    Gadaleta, Alessandro; Biance, Anne-Laure; Siria, Alessandro; Bocquet, Lyderic


    A challenge for the development of nanofluidics is to develop new instrumentation tools, able to probe the extremely small mass transport across individual nanochannels. Such tools are a prerequisite for the fundamental exploration of the breakdown of continuum transport in nanometric confinement. In this letter, we propose a novel method for the measurement of the hydrodynamic permeability of nanometric pores, by diverting the classical technique of Coulter counting to characterize a pressure-driven flow across an individual nanopore. Both the analysis of the translocation rate, as well as the detailed statistics of the dwell time of nanoparticles flowing across a single nanopore, allow us to evaluate the permeability of the system. We reach a sensitivity for the water flow down to a few femtoliters per second, which is more than two orders of magnitude better than state-of-the-art alternative methods.

  15. Microfluidic and Nanofluidic Resistive Pulse Sensing: A Review

    Directory of Open Access Journals (Sweden)

    Yongxin Song


    Full Text Available The resistive pulse sensing (RPS method based on the Coulter principle is a powerful method for particle counting and sizing in electrolyte solutions. With the advancement of micro- and nano-fabrication technologies, microfluidic and nanofluidic resistive pulse sensing technologies and devices have been developed. Due to the unique advantages of microfluidics and nanofluidics, RPS sensors are enabled with more functions with greatly improved sensitivity and throughput and thus have wide applications in fields of biomedical research, clinical diagnosis, and so on. Firstly, this paper reviews some basic theories of particle sizing and counting. Emphasis is then given to the latest development of microfuidic and nanofluidic RPS technologies within the last 6 years, ranging from some new phenomena, methods of improving the sensitivity and throughput, and their applications, to some popular nanopore or nanochannel fabrication techniques. The future research directions and challenges on microfluidic and nanofluidic RPS are also outlined.

  16. Particle size analysis of sediments, soils and related particulate materials for forensic purposes using laser granulometry. (United States)

    Pye, Kenneth; Blott, Simon J


    Particle size is a fundamental property of any sediment, soil or dust deposit which can provide important clues to nature and provenance. For forensic work, the particle size distribution of sometimes very small samples requires precise determination using a rapid and reliable method with a high resolution. The Coulter trade mark LS230 laser granulometer offers rapid and accurate sizing of particles in the range 0.04-2000 microm for a variety of sample types, including soils, unconsolidated sediments, dusts, powders and other particulate materials. Reliable results are possible for sample weights of just 50 mg. Discrimination between samples is performed on the basis of the shape of the particle size curves and statistical measures of the size distributions. In routine forensic work laser granulometry data can rarely be used in isolation and should be considered in combination with results from other techniques to reach an overall conclusion.

  17. Survival and activity of Streptococcus faecalis and escherichia coli in tropical freshwater

    International Nuclear Information System (INIS)

    Muniz, I; Toranzos, G.A.; Jimenez, L.; Hazen, T.C.


    The survival of Streptococcus faecalis and Escherichia coli was studied in situ in a tropical rain forest watershed using membrane diffusion chambers. Densities were determined by acridine orange direct count and Coulter Counter. Population activity was determined by microautoradiography, cell respiration, and by nucleic acid composition. Densities of S. faecalis and E. coli decreased less than 1 log unit after 105 hours as measured by direct count methods. Activity as measured by respiration, acridine orange activity, and microautoradiography indicated that both bacteria remained moderately active during the entire study. After 12 hours, E. coli was more active than S. faecalis as measured by nucleic acid composition. In this tropical rain forest watershed, E. coli and S. faecalis survived and remained active for more than 5 days; consequently, both would seem to be unsuitable as indicators of recent fecal contamination in tropical waters

  18. Effect of sex, age, number of bronchoalveolar lavages and quantitation methods on the bronchoalveolar cell counts in rats. (United States)

    Lopez, A; Yong, S; Sharma, A; Bailey, D


    This study was conducted to investigate the effect of sex, age, number of bronchoalveolar lavages and method of quantitation on the number of bronchoalveolar cells in rats. Forty Long Evans rats were divided into four age-sex subgroups of ten animals each. Nine consecutive bronchoalveolar lavages were done in every rat and the number of bronchoalveolar cells/mL in lavages 1-3 (L1), 4-6 (L2) and 7-9 (L3) were determined by hemocytometer and electronic cell counts (Coulter Counter). The sex or age of the rats did not show a significant effect (p less than 0.05) in the number of bronchoalveolar cells recovered from the lungs; however, there was a significant difference (p less than 0.05) in the number of cells/mL among lavages 1-3, 4-6 and 7-9 (L1 approximately equal to L2 greater than L3). A discrepancy of approximately 8% in the counts of bronchoalveolar cells was found between the hemocytometer and the Coulter Counter; however, these two methods of cell quantitation showed a significant (p less than 0.01) positive correlation (r = 0.89). No significant (p greater than 0.05) differences were found in the percentage of fluid recovery (overall mean = 94.5%) among lavages L1, L2 and L3. It was concluded that the electronic cell counting of bronchoalveolar cells is as reliable as manual counting. Although sex or age did not significantly affect the number of cells recovered from the lung, caution should be used in the number of lavages done per rat since this variable may significantly affect the results. PMID:3742347


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    Full Text Available We describe a case of human T-lymphotropic virus type I associated myelopathy in a 50-year old woman in Nigeria. The patient presented with progressive loss of tone to the two lower limbs and later inability to walk. The HTLV-I antibody presence in the plasma collected from the patient was repeatedly detected by enzyme immunoassays (Abbott HTLV-I EIA and Coulter SELECT-HTLV I/II and confirmed by Western blot technique. In addition, HTLV-I DNA was amplified from the genomic DNA isolated from the peripheral blood mononuclear cells of the patient by the polymerase chain reaction technique. This finding is significant being the first report of association of HTLV-I with myelopathy in Nigeria.Descrevemos um caso de infecção por HTLV-I associado a mielopatia, em mulher de 50 anos, na Nigéria. A paciente apresentou fraqueza progressiva dos membros inferiores e posteriormente incapacidade para andar. A presença de anticorpo HTLV-I no plasma coletado da paciente foi repetidamente detectada pelos ensaios imunoenzimáticos (Abbott HTLV-I EIA e Coulter SELECT-HTLV I/II e confirmada pela técnica de Western Blot. Adicionalmente amplificou-se o DNA do HTLV-I a partir do DNA genômico isolado das células mononucleares do sangue periférico da paciente através da técnica PCR. Este achado é significativo sendo o primeiro relato de associação de HTLV-I com mielopatia, na Nigéria.

  20. Construction of an experimental plot seeder of wheat planting and compare it by imported one

    Directory of Open Access Journals (Sweden)

    I Eskandari


    Full Text Available Introduction Researchers frequently include multiple cultivars and fertility levels in field experiments. Therefore, the experiments sowing operation must represent a considerable saving in time and labor, compared to hand sowing. Greater flexibility in experimental design and setup could be achieved by equipment that enables quick changes in the cultivar and fertilizer rates from one plot to the next. A satisfactory seed drill must distribute a given quantity of seed evenly over a predetermined length of coulter row, the coulters must be spaced at exact intervals and depth of sowing must be uniform. In a self-propelled type of plot seeder, no coulter should run in a wheel track as the compaction of the soil can cause observable differences in vigor between plants in such a row and those in un-compacted rows. The machine should sow in succession from a try in which a series of seed pocket separated clearly and must be put into distributer funnel by an assistant operator. The length of gap being varied according to the nature and purpose of the plot. The objectives of this experiment were 1- to design and construct a local self-propelled plot seeder and 2- To compare it with the imported (Wintersteiger plot seeder in cereal breeding programs. Materials and Methods A small-plot seeder was designed and constructed to meet this objective. The unit consists of the following basic components: a toolbar for pulling a set of six blade coulter, an air compressor for lifting and putting down the openers and metering transmission drive wheel, an operators chair and work rack, one belt seed distribution. A cone-celled and rotor seed distributor is used for seed distribution to the openers. The cone system is connected to the gearbox and allows for great flexibility in changing cultivars, crop species, and plot length. This is driven by the separate drive wheel. The cone-celled distributor sows all the seed of the sample in making one complete turn. The

  1. Thyroglobulin (Tg) Testing Revisited: Tg Assays, TgAb Assays, and Correlation of Results With Clinical Outcomes. (United States)

    Netzel, Brian C; Grebe, Stefan K G; Carranza Leon, B Gisella; Castro, M Regina; Clark, Penelope M; Hoofnagle, Andrew N; Spencer, Carole A; Turcu, Adina F; Algeciras-Schimnich, Alicia


    Measurement of thyroglobulin (Tg) by mass spectrometry (Tg-MS) is emerging as a tool for accurate Tg quantification in patients with anti-Tg autoantibodies (TgAbs). The objective of the study was to perform analytical and clinical evaluations of two Tg-MS assays in comparison with immunometric Tg assays (Tg-IAs) and Tg RIAs (Tg-RIAs) in a cohort of thyroid cancer patients. A total of 589 samples from 495 patients, 243 TgAb-/252 TgAb+, were tested by Beckman, Roche, Siemens-Immulite, and Thermo-Brahms Tg and TgAb assays, two Tg-RIAs, and two Tg-MS assays. The frequency of TgAb+ was 58%, 41%, 27%, and 39% for Roche, Beckman, Siemens-Immulite, and Thermo-Brahms, respectively. In TgAb- samples, clinical sensitivities and specificities of 100% and 74%-100%, respectively, were observed across all assays. In TgAb+ samples, all Tg-IAs demonstrated assay-dependent Tg underestimation, ranging from 41% to 86%. In TgAb+ samples, the use of a common cutoff (0.5 ng/mL) for the Tg-MS, three Tg-IAs, and the USC-RIA improved the sensitivity for the Tg-MSs and Tg-RIAs when compared with the Tg-IAs. In up to 20% of TgAb+ cases, Tg-IAs failed to detect Tg that was detectable by Tg-MS. In Tg-RIAs false-high biases were observed in TgAb+ samples containing low Tg concentrations. Tg-IAs remain the method of choice for Tg quantitation in TgAb- patients. In TgAb+ patients with undetectable Tg by immunometric assay, the Tg-MS will detect Tg in up to 20% additional cases. The Tg-RIA will detect Tg in approximately 35% cases, but a significant proportion of these will be clinical false-positive results. The undetectable Tg-MS seen in approximately 40% of TgAb+ cases in patients with disease need further evaluation.

  2. Validação de tecnologia 5diff do analisador hematológico Sysmex XS-1000i para laboratório de pequeno/médio porte Validation of 5diff technologies of the Sysmex XS-1000i hematology analyzer for small and medium laboratories

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    Luiz F. Borges


    Full Text Available O Sysmex XS-1000i® é um novo e compacto analisador hematológico com tecnologia 5diff e citometria de fluxo fluorescente, tecnologias até então presentes em equipamentos de grande porte e disponíveis apenas para equivalentes rotinas laboratoriais. Neste estudo avaliamos seu desempenho com equipamento de análise de impedãncia (Coulter T-890® e à contagem diferencial de 200 leucócitos em microscópio. Foram analizadas 100 amostras paralelamente nos dois equipamentos e submetidas à contagem diferencial de 200 células em distensão sanguínea. Os resultados foram analisados mediante análise de correlação de Spearman para dados não paramétricos, mostrando correlação superior a 95% para os parâmetros WBC, RBC, hemoglobina, hematócrito e neutrófilos; correlação entre 90% e 94,9% para VCM, HCM, linfócitos e eosinófilos ; correlação inferior a 89,9 % para CHCM, monócitos e basófilos. Os resultados obtidos sugerem que a tecnologia embarcada neste equipamento é uma versátil ferramenta para o analista na rotina de pequeno e médio porte. No entanto, em caso de emissão de "flags" não dispensa totalmente a confirmação ao microscópio para garantia da qualidade final do exame.The Sysmex XS-1000i® is a new and compact blood analyzer with 5diff technology and fluorescent flow cytometry, technologies hitherto only present in large equipment and available only for laboratory equivalent routines. In this study its performance was compared with equipment for impedance analysis (Coulter T-890® and the microscopic counting of 200 differential leukocytes. One hundred samples were evaluated in parallel by both apparatuses and subjected to differential counts of 200 cells. The results were analyzed using the Spearman correlation for parametric data showing no correlation greater than 95% for white blood cells, red blood cells, hemoglobin, hematocrit and neutrophils,; correlations between 90% and 94.9% for VCM, MCH, lymphocytes and

  3. Automated haematology analysis to diagnose malaria

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    Grobusch Martin P


    Full Text Available Abstract For more than a decade, flow cytometry-based automated haematology analysers have been studied for malaria diagnosis. Although current haematology analysers are not specifically designed to detect malaria-related abnormalities, most studies have found sensitivities that comply with WHO malaria-diagnostic guidelines, i.e. ≥ 95% in samples with > 100 parasites/μl. Establishing a correct and early malaria diagnosis is a prerequisite for an adequate treatment and to minimizing adverse outcomes. Expert light microscopy remains the 'gold standard' for malaria diagnosis in most clinical settings. However, it requires an explicit request from clinicians and has variable accuracy. Malaria diagnosis with flow cytometry-based haematology analysers could become an important adjuvant diagnostic tool in the routine laboratory work-up of febrile patients in or returning from malaria-endemic regions. Haematology analysers so far studied for malaria diagnosis are the Cell-Dyn®, Coulter® GEN·S and LH 750, and the Sysmex XE-2100® analysers. For Cell-Dyn analysers, abnormal depolarization events mainly in the lobularity/granularity and other scatter-plots, and various reticulocyte abnormalities have shown overall sensitivities and specificities of 49% to 97% and 61% to 100%, respectively. For the Coulter analysers, a 'malaria factor' using the monocyte and lymphocyte size standard deviations obtained by impedance detection has shown overall sensitivities and specificities of 82% to 98% and 72% to 94%, respectively. For the XE-2100, abnormal patterns in the DIFF, WBC/BASO, and RET-EXT scatter-plots, and pseudoeosinophilia and other abnormal haematological variables have been described, and multivariate diagnostic models have been designed with overall sensitivities and specificities of 86% to 97% and 81% to 98%, respectively. The accuracy for malaria diagnosis may vary according to species, parasite load, immunity and clinical context where the

  4. Mecanismos de abertura do sulco e adubação nitrogenada no cultivo do feijoeiro em sistema plantio direto Furrows opening mechanism for nitrogen fertilizer application in common bean crop under no-tillage

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    Orivaldo Arf


    Full Text Available Algumas culturas têm pouca adaptação ao sistema plantio direto, em vista da maior compactação da camada superficial do solo e, nesse caso, o mecanismo utilizado na semeadora para a abertura dos sulcos para deposição do fertilizante pode ter grande importância no sentido de facilitar a penetração das raízes. Este experimento foi desenvolvido em Selvíria (MS, com o objetivo de avaliar a produtividade do feijão de inverno cultivado em sistema plantio direto, em função da utilização de mecanismos de abertura para distribuição de fertilizantes na semeadura e da adubação nitrogenada em cobertura. O delineamento experimental utilizado foi em blocos casualizados, utilizando-se esquema fatorial 2 x 6, constituído por mecanismos de distribuição de fertilizante (haste escarificadora e disco duplo e doses de N em cobertura (0, 25, 50, 75, 100 e 125 kg ha-1, com quatro repetições. Recomenda-se o uso da haste escarificadora como mecanismo de distribuição do fertilizante, para o cultivo do feijoeiro de inverno. A adubação nitrogenada em cobertura proporciona incrementos à produtividade do feijoeiro de inverno.Some crops have shown no adaptation to no-tillage system as a function of compaction soil superficial layer. In way, the mechanism used in seeder to open furrows for deposition of fertilizer can have great importance to facilitate the penetration of roots. This experiment was carried in Selvíria (MS, with the objective to evaluate the winter common bean crop yield under no-tillage system, as function of fertilizer distribution opening mechanisms in sowing (chisel and coulter blade and sidedressing nitrogen application (0, 25, 50, 75, 100 and 125 kg ha-1. The experimental design was a randomized block, arranged in a 2 x 6 factorial scheme, constituted by fertilizer distribution opening mechanisms in sowing (chisel and coulter blade and sidedressing nitrogen doses (0, 25, 50, 75, 100 and 125 kg ha-1, with four replications

  5. Anemia em escolares da primeira série do ensino fundamental da rede pública de Maceió, Alagoas, Brasil Anemia in public school first graders in the city of Maceió, Alagoas, Brazil

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    Célia Dias dos Santos


    Full Text Available Realizou-se um estudo transversal com amostra probabilística de 426 alunos de 6 a 10 anos, da 1ª série do ensino fundamental das escolas públicas de Maceió, Alagoas, Brasil. O objetivo foi avaliar a prevalência da anemia e sua associação com o retardo de crescimento. Os dados foram coletados entre maio e julho de 2000. O sangue foi colhido por venipuntura e a dosagem de hemoglobina (Hb foi realizada pelo contador Coulter STKS. Classificou-se a anemia por dois critérios estabelecidos pela Organização Mundial da Saúde: Hb A cross-sectional study was conducted in a representative sample of 426 randomly selected first graders (ages 6 to 10 years from public schools in Maceió, State of Alagoas, Brazil. The aim was to determine the prevalence of anemia, as well as its association with growth retardation. Data were collected from May to July 2000, and determination of hemoglobin (HGB employed an STKS Coulter counter. Two cut-off points were used to classify anemia, both established by the World Health Organization: HGB < 11.5g/dl and HGB < 12.0g/dl. The indicators height/age (H/A, weight/age (W/A, and weight/height (W/H below -2.0 standard deviations from the NCHS reference were diagnosed as growth retardation. Prevalence of anemia was 9.9% when HGB < 11.5g/dl was used, and 25.4% when the cut-off point was HGB < 12.0g/dl. Growth retardation was detected in 6.2% of children according to H/A, 4.0% for W/A, and 3.0% for W/H. There was no statistically significant association between the variables in the study. These findings confirm results of previous surveys where prevalence of anemia was much higher than that of growth retardation. The severe consequences of anemia in this age group justify the implementation of broad public policies to overcome this nutritional deficiency.

  6. Índices eritrocitarios en la esferocitosis hereditaria Erythrocyte indexes in hereditary spherocytosis

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    Silvia Eandi Eberle


    Full Text Available La esferocitosis hereditaria es un grupo heterogéneo de desórdenes caracterizados por la variabilidad en la clínica, en los defectos proteicos del citoesqueleto eritrocitario y en el tipo de herencia. Se estudió la sensibilidad y especificidad de la concentración de hemoglobina corpuscular media (CHCM y el índice de amplitud de distribución eritrocitaria (ADE en el screening diagnóstico de la esferocitosis hereditaria. Noventa y cuatro pacientes fueron comparados con niños sanos de igual sexo y edad. Todos los índices se obtuvieron por impedancia eléctrica (autoanalizador hematológico Coulter JT. En los pacientes con esferocitosis hereditaria, la CHCM (35.67±1.33 g/dl y el ADE (20.60±4.5%, fueron significativamente más elevados que en el grupo control (CHCM 33.48±0.68 g/dl, p 0.000; ADE 13.22±0.9%, p 0.000. Con los valores de corte utilizados en nuestro laboratorio (CHCM ≥ 34.5 g/dl; ADE ≥ 14.5% ambos índices elevados mostraron una sensibilidad de 81% y una especificidad de 98.9% en el screening de esferocitosis hereditaria. La combinación de ambos índices es un excelente predictor para el diagnóstico de esferocitosis hereditaria.Hereditary spherocytosis is a group of heterogenous disorders characterized by variability in its clinical manifestations, membrane protein defects and inheritance. We analysed the sensitivity and specificity of mean corpuscular hemoglobin concentration (MCHC and red cell distribution width (RDW in the diagnostic screening of hereditary spherocytosis. Ninetyfour patients were compared to equal number of healthy, age-matched children. All indexes were derived from measurements obtained by aperture impedance (Coulter Counter Model JT. In patients with hereditary spherocytosis, MCHC (35.67±1.33 g/dl and RDW (20.60±4.5% were significantly higher than in normal control subjects (MCHC 33.48±0.68 g/dl, p: 0.000; RDW 13.22±0.9%, p: 0.000. By using a cutoff for the MCHC of 34.5 g/dl and for the RDW

  7. Roger Hayward and the Invention of the Two-Mirror Schmidt (United States)

    Bell, T. E.


    Roger Hayward (1899-1979), now virtually unknown, was a multitalented architect, scientific illustrator, and optical inventor. Remembered primarily for illustrating Scientific American magazine's Amateur Scientist column between 1949 and 1974, he also illustrated more than a dozen textbooks in optics, physics, geology, oceanography, and chemistry, several of which became classics in their fields. He designed façades with astronomical themes for major buildings in Los Angeles, California, and sculpted mammoth, realistic models of the moon for Griffith Observatory, Adler Planetarium, and Disneyland. Throughout his life, he recreationally painted watercolors and oils that at least one critic likened to the work of John Singer Sargent. Hayward is least known as an optical designer, yet he made significant contributions to the DU spectrophotometer that established the multimillion-dollar company Beckman Instruments. During the pre-radar days of World War II at Mount Wilson Observatory, Hayward invented a classified Cassegrain version of the Schmidt telescope especially adapted for nighttime infrared aerial photography, plus extraordinarily simple machines that allowed inexperienced soldiers to grind, polish, and test accurate aspheric Schmidt correcting plates at speeds compatible with mass production - and later received U.S. patents for them all. This paper, drawn in part from unpublished letters between Hayward and Albert G. Ingalls, will feature little-known images of Hayward's work.

  8. Cytotoxic effect of Erythroxylum suberosum combined with radiotherapy in head and neck cancer cell lines

    International Nuclear Information System (INIS)

    Macedo, Taysa B.C.; Torres, Hianne M.; Yamamoto-Silva, Fernanda Paula; Silva, Maria Alves G.; Elias, Silvia T.; Silveira, Damaris; Magalhaes, Perola O.; Lofrano-Porto, Adriana; Guerra, Eliete N.S.


    The mouth and oropharynx cancer is the 6 th most common type of cancer in the world. The treatment may involve surgery, chemotherapy and radiotherapy. More than 50% of drugs against cancer were isolated from natural sources, such as Catharanthus roseus and epipodophyllotoxin, isolated from Podophyllum. The biggest challenge is to maximize the control of the disease, while minimizing morbidity and toxicity to the surrounding normal tissues. The Erythroxylum suberosum is a common plant in the Brazilian Cerrado biome and is popularly known as 'cabelo-de-negro'. The objective of this study was to evaluate the cytotoxic activity of Erythroxylum suberosum plant extracts of the Brazilian Cerrado biome associated with radiotherapy in human cell lines of oral and hypopharynx carcinomas. Cells were treated with aqueous, ethanolic and hexanic extracts of Erythroxylum suberosum and irradiated at 4 Gy, 6 Gy and 8 Gy. Cytotoxicity was evaluated by MTT assay and the absorbance was measured at 570 nm in a Beckman Counter reader. Cisplatin, standard chemotherapy, was used as positive control. The use of Erythroxylum suberosum extracts showed a possible radiosensitizing effect in vitro for head and neck cancer. The cytotoxicity effect in the cell lines was not selective and it is very similar to the effect of standard chemotherapy. The aqueous extract of Erythroxylum suberosum, combined with radiotherapy was the most cytotoxic extract to oral and hypopharynx carcinomas. (author)

  9. Preparation and infrared spectra of differently deuterated tetramethyl-derivatives of the IV. main group

    International Nuclear Information System (INIS)

    Biedermann, S.


    19 different deuterated tetramethyl derivates of the type (CH 3 )sub(4-n)M(CH 3 )sub(n) of C, Si, Ge, Sn and Pb were prepared. Gas cuvettes with polyethylene, NaCl and KBr windows were used to absorb the infra-red spectra, the IR equipment Beckman IR 11 and IR 12 were used. The infra-red spectra of the above mentioned compounds were indicated from 33 to 4,000 cm -1 , the ground, upper and combination vibrations assigned, the PR separations of the partly well resolved rotation-vibration outlines determined and were compared with the calculated PR separations. The revision of the correlations performed by Graham for γsub(s)CH 3 and γsub(as)CH 3 in the race of vibration F 2 with Sn(CH 3 ) 4 and Pb(CH 3 ) 4 and the proposed one with C(CH 3 ) 4 , Si(CH 3 ) 4 and Ge(CH 3 ) 4 could be disproved by the new experimental results. (FW) [de

  10. Biomedical optics centers: forty years of multidisciplinary clinical translation for improving human health (United States)

    Tromberg, Bruce J.; Anderson, R. Rox; Birngruber, Reginald; Brinkmann, Ralf; Berns, Michael W.; Parrish, John A.; Apiou-Sbirlea, Gabriela


    Despite widespread government and public interest, there are significant barriers to translating basic science discoveries into clinical practice. Biophotonics and biomedical optics technologies can be used to overcome many of these hurdles, due, in part, to offering new portable, bedside, and accessible devices. The current JBO special issue highlights promising activities and examples of translational biophotonics from leading laboratories around the world. We identify common essential features of successful clinical translation by examining the origins and activities of three major international academic affiliated centers with beginnings traceable to the mid-late 1970s: The Wellman Center for Photomedicine (Mass General Hospital, USA), the Beckman Laser Institute and Medical Clinic (University of California, Irvine, USA), and the Medical Laser Center Lübeck at the University of Lübeck, Germany. Major factors driving the success of these programs include visionary founders and leadership, multidisciplinary research and training activities in light-based therapies and diagnostics, diverse funding portfolios, and a thriving entrepreneurial culture that tolerates risk. We provide a brief review of how these three programs emerged and highlight critical phases and lessons learned. Based on these observations, we identify pathways for encouraging the growth and formation of similar programs in order to more rapidly and effectively expand the impact of biophotonics and biomedical optics on human health.

  11. Analytical and clinical performance of the new Fujirebio 25-OH vitamin D assay, a comparison with liquid chromatography-tandem mass spectrometry (LC-MS/MS) and three other automated assays. (United States)

    Saleh, Lanja; Mueller, Daniel; von Eckardstein, Arnold


    We evaluated the analytical and clinical performance of the new Lumipulse® G 25-OH vitamin D assay from Fujirebio, and compared it to a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and three other commercial automated assays. Total 25 hydroxy vitamin D (25(OH)D) levels were measured in 100 selected serum samples from our routine analysis with Fujirebio 25(OH)D assay. The results were compared with those obtained with LC-MS/MS and three other automated 25(OH)D assays (Abbott, Beckman, and Roche). The accuracy of each assay tested was evaluated against a Labquality reference serum panel for 25(OH)D (Ref!25OHD; University of Ghent). Intra- and inter-day imprecision of the Fujirebio 25(OH)D assay was Lumipulse G 25-OH vitamin D assay from Fujirebio demonstrated a good correlation with LC-MS/MS and some immunoassays. The performance of the assay is well-suited for routine 25(OH)D measurement in clinical serum samples. A correction for the observed negative bias vs. LC-MS/MS could be considered.

  12. Phonon Surface Scattering and Thermal Energy Distribution in Superlattices. (United States)

    Kothari, Kartik; Maldovan, Martin


    Thermal transport at small length scales has attracted significant attention in recent years and various experimental and theoretical methods have been developed to establish the reduced thermal conductivity. The fundamental understanding of how phonons move and the physical mechanisms behind nanoscale thermal transport, however, remains poorly understood. Here we move beyond thermal conductivity calculations and provide a rigorous and comprehensive physical description of thermal phonon transport in superlattices by solving the Boltzmann transport equation and using the Beckman-Kirchhoff surface scattering theory with shadowing to precisely describe phonon-surface interactions. We show that thermal transport in superlattices can be divided in two different heat transport modes having different physical properties at small length scales: layer-restricted and extended heat modes. We study how interface conditions, periodicity, and composition can be used to manipulate the distribution of thermal energy flow among such layer-restricted and extended heat modes. From predicted frequency and mean free path spectra of superlattices, we also investigate the existence of wave effects. The results and insights in this paper advance the fundamental understanding of heat transport in superlattices and the prospects of rationally designing thermal systems with tailored phonon transport properties.

  13. Failure rate analysis using GLIMMIX

    International Nuclear Information System (INIS)

    Moore, L.M.; Hemphill, G.M.; Martz, H.F.


    This paper illustrates use of a recently developed SAS macro, GLIMMIX, for implementing an analysis suggested by Wolfinger and O'Connell (1993) in modeling failure count data with random as well as fixed factor effects. Interest in this software tool arose from consideration of modernizing the Failure Rate Analysis Code (FRAC), developed at Los Alamos National Laboratory in the early 1980's by Martz, Beckman and McInteer (1982). FRAC is a FORTRAN program developed to analyze Poisson distributed failure count data as a log-linear model, possibly with random as well as fixed effects. These statistical modeling assumptions are a special case of generalized linear mixed models, identified as GLMM in the current statistics literature. In the nearly 15 years since FRAC was developed, there have been considerable advances in computing capability, statistical methodology and available statistical software tools allowing worthwhile consideration of the tasks of modernizing FRAC. In this paper, the approaches to GLMM estimation implemented in GLIMMIX and in FRAC are described and a comparison of results for the two approaches is made with data on catastrophic time-dependent pump failures from a report by Martz and Whiteman (1984). Additionally, statistical and graphical model diagnostics are suggested and illustrated with the GLIMMIX analysis results

  14. CLSI-based transference and verification of CALIPER pediatric reference intervals for 29 Ortho VITROS 5600 chemistry assays. (United States)

    Higgins, Victoria; Truong, Dorothy; Woroch, Amy; Chan, Man Khun; Tahmasebi, Houman; Adeli, Khosrow


    Evidence-based reference intervals (RIs) are essential to accurately interpret pediatric laboratory test results. To fill gaps in pediatric RIs, the Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) project developed an age- and sex-specific pediatric RI database based on healthy pediatric subjects. Originally established for Abbott ARCHITECT assays, CALIPER RIs were transferred to assays on Beckman, Roche, Siemens, and Ortho analytical platforms. This study provides transferred reference intervals for 29 biochemical assays for the Ortho VITROS 5600 Chemistry System (Ortho). Based on Clinical Laboratory Standards Institute (CLSI) guidelines, a method comparison analysis was performed by measuring approximately 200 patient serum samples using Abbott and Ortho assays. The equation of the line of best fit was calculated and the appropriateness of the linear model was assessed. This equation was used to transfer RIs from Abbott to Ortho assays. Transferred RIs were verified using 84 healthy pediatric serum samples from the CALIPER cohort. RIs for most chemistry analytes successfully transferred from Abbott to Ortho assays. Calcium and CO 2 did not meet statistical criteria for transference (r 2 CALIPER pediatric RI database to laboratories using Ortho VITROS 5600 biochemical assays. Clinical laboratories should verify CALIPER reference intervals for their specific analytical platform and local population as recommended by CLSI. Copyright © 2018 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  15. Color Parameters of the Chromascop Shade Guide

    Directory of Open Access Journals (Sweden)

    William J. O'Brien


    Full Text Available The purposes of this study are: (1 determine the color of the twenty shades in the Ivoclar’s Chromascop shade guide, (2 determine the color representation of the shade guide described as coverage error (CE, and (3 compare this shade guide with the Vita Classical and Bioform shade guides. The spectral data was collected using Beckman model DU reflectance spectrophotometer equipped with an integrating sphere. Commission International de l’Eclairage (CIE chromaticity coordinates were calculated using CIE illuminant C and 1931 observer data, then converted to CIE L*a*b* and Munsell notation. Each shade was spectrophotometrically compared to the published colors of 335 human teeth. The minimum CIE L*a*b* color difference was calculated for each tooth and the average of these color differences was defined as the CE. The measured colors of the Chromascop guide had a CIE L* range of 79.67 to 65.61, an a* range of -0.71 to 3.85, and a b* range of 14.58 to 27.69. The average CE of the Chromascop shade guide was 3.38. The Chromascop shade guide has similar colors and a CE compared with the Bioform and Vita Classical shade guides, but with some shades of higher red and yellow components.

  16. An application of the theory of planned behavior to examine the impact of classroom inclusion on elementary school students. (United States)

    Campbell, Michael


    Classroom inclusion serves as the most discussed service delivery model in the debate over the most appropriate way to provide education for students with disabilities. Integrating students with disabilities with nondisabled peers may increase attitudes of acceptance, but the literature also indicates that placement alone does not yield an increase in interaction between these two groups of peers (Brinker & Thorpe, 1986; Fryexe & Kennedy, 1995; Kennedy, Shulka, & Fryxell, 1997). This study investigated the impact of classroom inclusion on nondisabled students. Using survey research methods and guided by the Theory of Planned Behavior (Aizen, I. [1985]. From intentions to actions: A theory of planned behavior. In J. Kuhl & J. Beckman [Eds.], Action-control: From cognition to behavior [pp. 11-39]. Heidelberg: Springer), 593 responses were obtained from a convenience sample of 936 third, fourth, and fifth grade students and their parents from 52 classrooms spread across six different schools. Survey results were also collected from these students' parents and their teachers and used to add a richer depth to the data analysis. Implications for policy and practice are drawn. Limitations and recommendations for future research are also indicated.

  17. [Determination of ciprofloxacin in human serum and urine by reversed-phase HPLC]. (United States)

    Qin, Y; Liang, D


    A sensitive and rapid method for the determination of ciprofloxacin using enoxacin as the internal standard was reported. High-performance liquid chromatograph model 344 (Beckman, USA) with a variable wavelength UV detector and reversed-phase Ultrasphere-ODS column (5 microns, 250 x 4.6 mm) was used. Serum or urine sample preparation involved addition phosphate buffer (pH 7.5) and aqueous solution of sodium lauryl sulfate, followed by chloroform extraction. The organic layer was removed and evaporated to dryness under an air stream in a 37 degrees C water bath. The residue was dissolved in 50 microliters mobile phase and 20 microliters injected. The mobile phase of 0.02 mol/L acetate buffer (pH 3.0) -acetonitril-dimethylformamide-10% aqueous solution of tetrabutyl ammonium hydroxide (88:6.5:5:0.5) was pumped at 0.9 ml/min through the column. The detector operated at 0.01 aufs and the wavelength was set at 276 nm. The retention times for ciprofloxacin and enoxacin were 7.31 min and 5.59 min, respectively. In serum, standard curve was linear in the concentration range of 0.75 to 24 mumol/L, the detective limit was 0.2 mumol/L, extraction recovery was 69-74%, within-day CV was less than 5%, and inter-day CV was less than 6%.

  18. Pitch accent alignment in romance: primary and secondary associations with metrical structure. (United States)

    Prieto, Pilar; D'Imperio, Mariapaola; Fivela, Barbara Gili


    The article describes the contrastive possibilities of alignment of high accents in three Romance varieties, namely, Central Catalan, Neapolitan Italian, and Pisa Italian. The Romance languages analyzed in this article provide crucial evidence that small differences in alignment in rising accents should be encoded phonologically. To account for such facts within the AM model, the article develops the notion of "phonological anchoring" as an extension of the concept of secondary association originally proposed by Pierrehumbert and Beckman (1988), and later adopted by Grice (1995), Grice, Ladd, and Arvaniti (2000), and others to explain the behavior of edge tones. The Romance data represent evidence that not only peripheral edge tones seek secondary associations. We claim that the phonological representation of pitch accents should include two independent mechanisms to encode alignment properties with metrical structure: (1) encoding of the primary phonological association (or affiliation) between the tone and its tone-bearing unit; and (2), for some specific cases, encoding of the secondary phonological anchoring of tones to prosodic edges (moras, syllables, and prosodic words). The Romance data described in the article provide crucial evidence of mora-edge, syllable-edge, and word-edge H tonal associations.

  19. Cytotoxic effect of Erythroxylum suberosum combined with radiotherapy in head and neck cancer cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Macedo, Taysa B.C.; Torres, Hianne M.; Yamamoto-Silva, Fernanda Paula; Silva, Maria Alves G. [Universidade Federal de Goias (UFG), Goiania, GO (Brazil). Escola de Odontologia; Elias, Silvia T.; Silveira, Damaris; Magalhaes, Perola O.; Lofrano-Porto, Adriana; Guerra, Eliete N.S., E-mail: [Universidade de Brasilia (UnB), Brasilia, DF (Brazil). Faculdade de Ciencias da Saude


    The mouth and oropharynx cancer is the 6{sup th} most common type of cancer in the world. The treatment may involve surgery, chemotherapy and radiotherapy. More than 50% of drugs against cancer were isolated from natural sources, such as Catharanthus roseus and epipodophyllotoxin, isolated from Podophyllum. The biggest challenge is to maximize the control of the disease, while minimizing morbidity and toxicity to the surrounding normal tissues. The Erythroxylum suberosum is a common plant in the Brazilian Cerrado biome and is popularly known as 'cabelo-de-negro'. The objective of this study was to evaluate the cytotoxic activity of Erythroxylum suberosum plant extracts of the Brazilian Cerrado biome associated with radiotherapy in human cell lines of oral and hypopharynx carcinomas. Cells were treated with aqueous, ethanolic and hexanic extracts of Erythroxylum suberosum and irradiated at 4 Gy, 6 Gy and 8 Gy. Cytotoxicity was evaluated by MTT assay and the absorbance was measured at 570 nm in a Beckman Counter reader. Cisplatin, standard chemotherapy, was used as positive control. The use of Erythroxylum suberosum extracts showed a possible radiosensitizing effect in vitro for head and neck cancer. The cytotoxicity effect in the cell lines was not selective and it is very similar to the effect of standard chemotherapy. The aqueous extract of Erythroxylum suberosum, combined with radiotherapy was the most cytotoxic extract to oral and hypopharynx carcinomas. (author)

  20. CUSUM-Logistic Regression analysis for the rapid detection of errors in clinical laboratory test results. (United States)

    Sampson, Maureen L; Gounden, Verena; van Deventer, Hendrik E; Remaley, Alan T


    The main drawback of the periodic analysis of quality control (QC) material is that test performance is not monitored in time periods between QC analyses, potentially leading to the reporting of faulty test results. The objective of this study was to develop a patient based QC procedure for the more timely detection of test errors. Results from a Chem-14 panel measured on the Beckman LX20 analyzer were used to develop the model. Each test result was predicted from the other 13 members of the panel by multiple regression, which resulted in correlation coefficients between the predicted and measured result of >0.7 for 8 of the 14 tests. A logistic regression model, which utilized the measured test result, the predicted test result, the day of the week and time of day, was then developed for predicting test errors. The output of the logistic regression was tallied by a daily CUSUM approach and used to predict test errors, with a fixed specificity of 90%. The mean average run length (ARL) before error detection by CUSUM-Logistic Regression (CSLR) was 20 with a mean sensitivity of 97%, which was considerably shorter than the mean ARL of 53 (sensitivity 87.5%) for a simple prediction model that only used the measured result for error detection. A CUSUM-Logistic Regression analysis of patient laboratory data can be an effective approach for the rapid and sensitive detection of clinical laboratory errors. Published by Elsevier Inc.

  1. Biomedical optics centers: forty years of multidisciplinary clinical translation for improving human health. (United States)

    Tromberg, Bruce J; Anderson, R Rox; Birngruber, Reginald; Brinkmann, Ralf; Berns, Michael W; Parrish, John A; Apiou-Sbirlea, Gabriela


    Despite widespread government and public interest, there are significant barriers to translating basic science discoveries into clinical practice. Biophotonics and biomedical optics technologies can be used to overcome many of these hurdles, due, in part, to offering new portable, bedside, and accessible devices. The current JBO special issue highlights promising activities and examples of translational biophotonics from leading laboratories around the world. We identify common essential features of successful clinical translation by examining the origins and activities of three major international academic affiliated centers with beginnings traceable to the mid-late 1970s: The Wellman Center for Photomedicine (Mass General Hospital, USA), the Beckman Laser Institute and Medical Clinic (University of California, Irvine, USA), and the Medical Laser Center Lübeck at the University of Lübeck, Germany. Major factors driving the success of these programs include visionary founders and leadership, multidisciplinary research and training activities in light-based therapies and diagnostics, diverse funding portfolios, and a thriving entrepreneurial culture that tolerates risk. We provide a brief review of how these three programs emerged and highlight critical phases and lessons learned. Based on these observations, we identify pathways for encouraging the growth and formation of similar programs in order to more rapidly and effectively expand the impact of biophotonics and biomedical optics on human health.

  2. Use of CE-SDS gel for characterization of monoclonal antibody hinge region clipping due to copper and high pH stress. (United States)

    Rustandi, Richard R; Wang, Yang


    CE-SDS gel technique has been used extensively in the field of monoclonal antibody (mAb) as a tool for product purity, stability, and characterization. It offers many advantages over the traditional labor-intensive SDS-PAGE slab gel technology with respect to speed and resolution. Monoclonal antibodies are known to cleave in the hinge region due to extreme pH, high temperature and in the presence of metals, especially copper. This cleavage will impact the shelf lifetime of mAb product hence its quality. CESDS gel method using Beckman PA800 with UV detection is used to characterize the effects of copper and other metals such as iron and zinc on mAb clipping. In addition, mAb integrity under high temperature and high pH stress conditions was also evaluated and the results clearly show that CE-SDS gel can distinguish clipping due to copper versus heat and/or high pH. The data presented illustrate the power of this simple CESDS gel technique in supporting the development of mAb from product quality and stability to the final product characterization.

  3. Goeckerman's therapy for psoriasis with special reference to serum pentraxin 3 level

    Energy Technology Data Exchange (ETDEWEB)

    Ctirad, A.; Lenka, B.; David, P.; Zdenek, F.; Kveta, H.; Karel, E.; Jan, K. [Charles University Prague, Hradec Kralove (Czech Republic). University Hospital


    Goeckerman's therapy (GT) of psoriasis is based on daily application of pharmacy grade coal tar on affected skin with subsequent exposure to UV light. Pentraxin 3 (PTX3) is a newly identified acute phase reactant with non redundant functions in innate immunity. PTX3 has been shown to be a reliable prognostic marker in patients with various inflammatory disorders including rheumatoid arthritis, vasculitis, and psoriasis. The aim of this study was to evaluate the influence of Goeckerman's therapy of psoriasis on levels of two pentraxins: long pentraxin PTX3 and C reactive protein in 49 patients with chronic plaque psoriasis. CRP was assessed by immunonephelometry on IMMAGE 800 (Beckman, USA). PTX3 was detected using sandwich ELISA detection set (Alexis Biochemicals, Switzerland). The serum levels of both parameters (expressed as average {+-} 1 SD) were significantly diminished after GT. The level of PTX3 dropped from 1.92 {+-} 0.72 ng/ml before GT to 1.66 {+-} 0.58 ng/ml after GT (P = 0.0396) and the level of CRP fell from 4.64 {+-} 3.93 mg/l to 1.66 {+-} 0.58 mg/l (P {lt} 0.0001). Comparing to healthy controls, the serum levels of both parameters before GT were significantly higher than those found in healthy blood donors and remained significantly increased after GT. Increased serum concentrations of pentraxin 3 and CRP are alleviated by GT in patients with psoriasis.

  4. Harmonisation of seven common enzyme results through EQA. (United States)

    Weykamp, Cas; Franck, Paul; Gunnewiek, Jacqueline Klein; de Jonge, Robert; Kuypers, Aldy; van Loon, Douwe; Steigstra, Herman; Cobbaert, Christa


    Equivalent results between different laboratories enable optimal patient care and can be achieved with harmonisation. We report on EQA-initiated national harmonisation of seven enzymes using commutable samples. EQA samples were prepared from human serum spiked with human recombinant enzymes. Target values were assigned with the IFCC Reference Measurement Procedures. The same samples were included at four occasions in the EQA programmes of 2012 and 2013. Laboratories were encouraged to report IFCC traceable results. A parallel study was done to confirm commutability of the samples. Of the 223 participating laboratories, 95% reported IFCC traceable results, ranging from 98% (ASAT) to 87% (amylase). Users of Roche and Siemens (97%) more frequently reported in IFCC traceable results than users of Abbott (91%), Beckman (90%), and Olympus (87%). The success of harmonisation, expressed as the recovery of assigned values and the inter-laboratory CV was: ALAT (recovery 100%; inter-lab CV 4%), ASAT (102%; 4%), LD (98%; 3%), CK (101%; 5%), GGT (98%; 4%), AP (96%; 6%), amylase (99%; 4%). There were no significant differences between the manufacturers. Commutability was demonstrated in the parallel study. Equal results in the same sample in the 2012 and 2013 EQA programmes demonstrated stability of the samples. The EQA-initiated national harmonisation of seven enzymes, using stable, commutable human serum samples, spiked with human recombinant enzymes, and targeted with the IFCC Reference Measurement Procedures, was successful in terms of implementation of IFCC traceable results (95%), recovery of the target (99%), and inter-laboratory CV (4%).

  5. Cytotoxic Effect of Erythroxylum suberosum Combined with Radiotherapy in Head and Neck Cancer Cell Lines. (United States)

    Macedo, Taysa B C; Elias, Silvia T; Torres, Hianne M; Yamamoto-Silva, Fernanda Paula; Silveira, Dâmaris; Magalhães, Pérola O; Lofrano-Porto, Adriana; Guerra, Eliete N S; Silva, Maria Alves G


    The mouth and oropharynx cancer is the 6th most common type of cancer in the world. The treatment may involve surgery, chemotherapy and radiotherapy. More than 50% of drugs against cancer were isolated from natural sources, such as Catharanthus roseus and epipodophyllotoxin, isolated from Podophyllum. The biggest challenge is to maximize the control of the disease, while minimizing morbidity and toxicity to the surrounding normal tissues. The Erythroxylum suberosum is a common plant in the Brazilian Cerrado biome and is popularly known as "cabelo-de-negro". The objective of this study was to evaluate the cytotoxic activity of Erythroxylum suberosum plant extracts of the Brazilian Cerrado biome associated with radiotherapy in human cell lines of oral and hypopharynx carcinomas. Cells were treated with aqueous, ethanolic and hexanic extracts of Erythroxylum suberosum and irradiated at 4 Gy, 6 Gy and 8 Gy. Cytotoxicity was evaluated by MTT assay and the absorbance was measured at 570 nm in a Beckman Counter reader. Cisplatin, standard chemotherapy, was used as positive control. The use of Erythroxylum suberosum extracts showed a possible radiosensitizing effect in vitro for head and neck cancer. The cytotoxicity effect in the cell lines was not selective and it is very similar to the effect of standard chemotherapy. The aqueous extract of Erythroxylum suberosum, combined with radiotherapy was the most cytotoxic extract to oral and hypopharynx carcinomas.

  6. Effects of dextran on five biuret-based procedures for total protein in serum. (United States)

    Barnes, D B; Pierce, G F; Lichti, D; Landt, M; Koenig, J; Chan, K M


    We evaluated the effect of dextran on values for total protein in serum as measured by the biuret method with five widely used automated instruments: the American Monitor Parallel; the Du Pont aca II; the Roche Cobas-Bio; the Kodak Ektachem 400; and the Beckman Astra 8. Dextran concentrations as great as 25 or 30 g/L had relatively little or no influence on total protein measurements by the latter three instruments. Dextran concentrations exceeding 6 g/L caused falsely low results with the aca, whereas the Parallel gave falsely high results when the dextran concentration exceeded 2 g/L. The aca total protein procedure could be protected from the interference by dextran concentrations up to 30 g/L by injecting 0.4-0.8 mL of ethylene glycol directly into the reagent pack before sampling. However, we could not eliminate the interference with the Parallel procedure by any simple means; we thus recommend that it not be used for measuring total protein in serum samples from patients who are being treated with dextran.

  7. Evaluation of a glucose meter against analytical quality specifications for hospital use. (United States)

    Singh Dhatt, Gurdeep; Agarwal, Mukesh; Bishawi, Bassam


    The value of glucose meters in point of care testing (POCT) by medical professionals and self monitoring of blood glucose (SMBG) by patients is well established. We evaluated the SureSteppFlexx glucose meter against objective targets for imprecision and total error (TE). The SureStepFlexx blood glucose system uses a reflectance-based glucose oxidase (GO) method and reports plasma-equivalent glucose values. The reference method was the Beckman LX20 Pro glucose oxidase/oxygen electrode method. Patient samples and commercial aqueous, quality control (QC) material were used to assess imprecision. To determine total error of the meters, results obtained on patient heparinized blood were compared against results obtained by the reference method using plasma. Analyses were carried out by an experienced nurse and technologist. Both operators achieved imprecision of American Diabetes Association (ADA) criteria are significantly lower. The SureSteppFlexx glucose meter meets analytical quality requirements and is suitable for POCT use in our hospital. We propose a tiered approach and suggest minimum, desirable and optimum total error targets for glucose meters of < or =5%, 7.9% and 13%, respectively.

  8. Traumatic floating clavicle: a case report and literature review. (United States)

    Gouse, Mohamad; Jacob, Korula Mani; Poonnoose, Pradeep Mathew


    Bipolar fracture dislocations of the clavicle are rare injuries, usually the result of high-energy direct trauma. Since the original description by Porral in 1831, only a handful of individual case reports and case series by Beckman and Sanders have been reported in the literature. Management of these injuries has remained controversial ranging from nonoperative to aggressive surgery. We report on the case of a young army cadet who had a fracture of the lateral end of the clavicle, with an anterior dislocation of the sternoclavicular joint. Despite being planned for surgery, at the patients request, it was decided to manage the lesion conservatively with graded physiotherapy. At one-year follow-up, he had full pain-free, functional range of movement of the shoulder. This young high demand patient had a good outcome with conservative management, despite going against the current trend towards surgical treatment. We present this case with a review of the literature, highlighting the various management options for this rare lesion.

  9. Amino acid composition of some Mexican foods. (United States)

    Morales de León, Josefina; Camacho, M Elena; Bourges, Héctor


    Knowledge of the amino acid composition of foods is essential to calculate their chemical score, which is used to predict protein quality of foods and diets. Though amino acid composition of many foods is reasonably well established, better knowledge is needed on native foods consumed in different regions and countries. This paper presents the amino acid composition of different presentations of raw and processed foods produced and consumed in Mexico. The amino acid composition was determined using Beckman amino acid analyzers (models 116 and 6300). Tryptophan was determined using the Spies and Chambers method. Of the different foods analyzed, some comments are made on native or basic foods in Mexico: Spirulin, where lysine is the limiting amino acid, with a chemical score of 67%, is a good source of tryptophan (1.16g/16 gN); amaranth contains high levels of sulphur amino acids (4.09 to 5.34 g/16gN), with a protein content of 15 g/100g; and pulque, a Pre-Hispanic beverage that contains high levels of tryptophan (2.58 g/16 gN) and sulphur amino acids (2.72 g/16 gN). Finally, insects are good sources of sulphur amino acids and lysine.

  10. Amino acid sequence of the signal peptide of apoVLDL-II, a major apoprotein in avian very low density lipoproteins. (United States)

    Chan, L; Bradley, W A; Means, A R


    ApoVLDL-II is a major apoprotein in avian very low density lipoproteins (Jackson, R. L., Lin, H.-Y., Chan, L., and Means, A.R. (1977) J. Biol. Chem. 252, 250-253). Partially purified apoVLDL-II mRNA was translated in vitro in a wheat germ system in the presence of various labeled amino acids. The product, designated preapoVLDL-II, was purified by immunoprecipitation and sodium dodecyl sulfate acrylamide gel electrophoresis. It was subjected to automated Edman degradation in a Beckman Sequencer. The signal peptide was found to be a 23-amino acid NH2-terminal extension of the mature protein with the following sequence: Met-Gln-Tyr-Arg-Ala-Leu-Val-Ile-Ala-Val-Ile-Leu-Leu-Leu-Ser-Thr-Val-Pro-Glu-Val-Cys-Ser-Lys where Lys is the NH2-terminal residue of mature apoVLDL-II. The abundance and distribution of the hydrophobic amino acid residues are very similar to those of other signal sequences and the average hydrophobicity for the 23 residues is -1227 cal/mol/residue. However, translocation of preapoVLDL-II would represent a unique case of vectorial migration of a protein through a membrane since apoVLDL-II is itself an apolipoprotein and binds lipid spontaneously.

  11. Smoking cessation: an application of theory of planned behavior to understanding progress through stages of change. (United States)

    Bledsoe, Linda K


    The purpose of this research was to investigate variables relevant to smoking cessation early in the process of change through an application of the Theory of Planned Behavior [Ajzen, I. (1985). From intentions to actions: A theory of planned behavior. In J. Kuhl and J. Beckman (Eds). Action-control: From cognition to behavior (pp.11-39). Heidelberg: Springer.] to the temporal structure provided by the Transtheoretical Model. Study 1 was a preliminary elicitation study (n=68) conducted to ground the concepts used in the model testing in Study 2 [Ajzen, I., Fishbein, M. (1980). Understanding attitudes and predicting social behavior, Englewood Cliffs, NJ: Prentice-Hall.]. Study 2 tested the proposed model fit with data from a sample of 230 adult smokers. Structural equation modeling did not support the Theory of Planned Behavior as a model of motivation for progress through the stages of change and highlighted measurement issues with perceived behavioral control. A modified model using the Theory of Reasoned Action provided a good fit to the data, accounting for approximately 64% of the variance in intention to quit smoking and stage of change. This research addresses the need for a more complete theoretical rationale for progress through stages of change.

  12. Extraction and analysis of Ursolic Acid from Spica Prunellae

    Directory of Open Access Journals (Sweden)

    Kai-quan LI


    Full Text Available Objective: To study the extract technology of Ursolic Acid from Spica Prunellae,and to build the method of determining its content. Methods: Adopting the method of ethanol extract and agglutination separate to isolate Ursolic Acid.The content of Ursolic Acid in sample is determined by HPLC with Methanol-H2O-Glacial acetic acid-Triethylamine (83:17:0.04:0.02 as mobile phase at a flow rate of 1.0ml/min,and detection wavelength at 210nm. Beckman ODS 150mm×4.6mm column is used at 25℃. Results: The product is recognized to be Ursolic Acid by physicochemical contents and thin layer chromatography.Adopting peak area normalization method for counting.The extraction rate is 99.8%. Conclusion: The technology of isolating and preparation Ursolic Acid is advanced、pratical、reasonable and feasible. It can be applied in industrial production. The purity of Ursolic Acid is high and the safety is good. The analytical method is simple and accurate.It can be adapted to evaluate the quality of Ursolic Acid.

  13. A Description of Tucumán Spanish Intonation in Argentina

    Directory of Open Access Journals (Sweden)

    Terán Virginia


    Full Text Available This paper documents for the first time the intonation system of Tucumán Spanish, an understudied variety of Argentinian Spanish. Semi-spontaneous speech illustrating the intonation of main sentence types, i.e. broad focus statements, partial and absolute interrogatives, and imperatives and vocatives, was elicited from 31 native speakers of Tucumán Spanish via an adapted version of the Argentinian Intonation Survey (Prieto and Roseano, 2009-2013. The two authors listened to the recordings and transcribed them using the Tones and Break Indexes conventions (ToBI (Beckman et al. 2002, Prieto and Roseano 2010, Hualde and Prieto 2015. Transcriptions of prenuclear and nuclear configurations together with their respective frequencies allowed both an appreciation of the most used configurations within each sentence type along with detailed variation at the phonetic level. For example, yes/no questions were consistently realized with a low nuclear pitch accent L* and an ascending boundary tone. However, there was variation in the height of the boundary tones yielding the frequent contour L* ¡H%, and the less frequent L* H%. Altogether, these detailed patterns document the systematic phonetic variation of the intonation system of TS and provide a basis for future research to determine the phonological status of this variation.

  14. Interspecific differences revealed with in Drosophila Photometric Analysis

    Directory of Open Access Journals (Sweden)

    Hoenigsberg H. F.


    Full Text Available The above refer to experiments present a new method which permits the study of philogenesis in the genus Drosophila. There are several types of results: a close kinship among the various geographical races of  D. melanogaster in the neo-tropics coincides with their spectrophotometric similarities; b the interspecific differences are also identified with the photometric analysis; c finally there are optical density affinities among the various species which belong to the same taxonomic groups.  Acknowledgment. The authors want to express their gratitude to Professor Everet of the physico-chemical laboratory of the National University for the use of his Beckman DU spectrophotometer and for his generous advice. This research is supported by the American Agricultural Research Service grant F. G. Co 107. For technical assistance we are indebted to Miss B. I. Cortés and to Mr. L. Castro.

  15. Physical properties and structure of fine core-shell particles used as packing materials for chromatography Relationships between particle characteristics and column performance. (United States)

    Gritti, Fabrice; Leonardis, Irene; Abia, Jude; Guiochon, Georges


    The recent development of new brands of packing materials made of fine porous-shell particles, e.g., Halo and Kinetex, has brought great improvements in potential column efficiency, demanding considerable progress in the design of chromatographic instruments. Columns packed with Halo and Kinetex particles provide minimum values of their reduced plate heights of nearly 1.5 and 1.2, respectively. These packing materials have physical properties that set them apart from conventional porous particles. The kinetic performance of 4.6mm I.D. columns packed with these two new materials is analyzed based on the results of a series of nine independent and complementary experiments: low-temperature nitrogen adsorption (LTNA), scanning electron microscopy (SEM), inverse size-exclusion chromatography (ISEC), Coulter counter particle size distributions, pycnometry, height equivalent to a theoretical plate (HETP), peak parking method (PP), total pore blocking method (TPB), and local electrochemical detection across the column exit section (LED). The results of this work establish links between the physical properties of these superficially porous particles and the excellent kinetic performance of columns packed with them. It clarifies the fundamental origin of the difference in the chromatographic performances of the Halo and the Kinetex columns. Copyright 2010 Elsevier B.V. All rights reserved.

  16. Encapsulation of Platelet in Kefiran Polymer and Detection of Bioavailability of Immobilized Platelet in Probiotic Kefiran as A New Drug for Surface Bleeding

    Directory of Open Access Journals (Sweden)

    Anahita Jenab


    Full Text Available Background : Kefir contains lactic acid bacteria (Lactobacillus, Lactococcus, Leuconostoc, Acetobacter and Streptococcus and yeasts (Kluyveromyces, Torula, Candida, Saccharomyces .Kefiran is the polysaccharide produced by lactic acid bacteria in kefir.Methods : Kefiran was prepared from milk containing 0.5% fat and 10 grams kefir grains and was separated from kefir by ethanol (0.02 gram following entrapping the platelets to this polymer. Ligand of the platelet-polysaccharide was studied by FTIR.Results : FTIR results showed that the bands of C-O and C-O-C connections were formed and the polysaccharides had been attached to the receptors of the platelet glycoproteins (GP Ib,GPIIb / IIIa. Stability and encapsulation of the platelet and kefiran were assessed by Coulter Counter. Encapsulation of the platelets by polysaccharide at the beginning caused to reduce the number of platelets following by releasing of 50% of the platelets after 3 hours.Conclusion : The platelets were encapsulated in kefiran polymer and detected for bioavailability as new drug for surface bleeding. Also, kefiran has antimicrobial and antifungal properties. On the other hand, the existence of nisin in kefiran could be useful as an antibacterial lantibiotic. 

  17. Analytical scale purification of zirconia colloidal suspension using field programmed sedimentation field flow fractionation. (United States)

    Van-Quynh, Alexandra; Blanchart, Philippe; Battu, Serge; Clédat, Dominique; Cardot, Philippe


    Sedimentation field flow fractionation was used to obtain purified fractions from a polydispersed zirconia colloidal suspension in the potential purpose of optical material hybrid coating. The zirconia particle size ranged from 50/70 nm to 1000 nm. It exhibited a log-Gaussian particle size distribution (in mass or volume) and a 115% polydispersity index (P.I.). Time dependent eluted fractions of the original zirconia colloidal suspension were collected. The particle size distribution of each fraction was determined with scanning electron microscopy and Coulter sub-micron particle sizer (CSPS). These orthogonal techniques generated similar data. From fraction average elution times and granulometry measurements, it was shown that zirconia colloids are eluted according to the Brownian elution mode. The four collected fractions have a Gaussian like distribution and respective average size and polydispersity index of 153 nm (P.I. = 34.7%); 188 nm (P.I. = 27.9%); 228 nm (P.I. = 22.6%), and 276 nm (P.I. = 22.3%). These data demonstrate the strong size selectivity of SdFFF operated with programmed field of exponential profile for sorting particles in the sub-micron range. Using this technique, the analytical production of zirconia of given average size and reduced polydispersity is possible.

  18. Soil structure and greenhouse gas production differences between row and interrow positions under no-tillage

    Directory of Open Access Journals (Sweden)

    Alvaro Pires da Silva


    Full Text Available No-tillage in Brazil is an efficient agricultural system that improves crop productivity whilst controlling erosion caused to the soil by degradation. However, there is some concern regarding soil compaction. Our objective was to determine whether the function of soil structure in sustaining crop growth was dependent on row and interrow positions in long-term no-tillage. We took soil samples from a field in a commercial farm under long-term no-tillage since 1979 on a clayey Oxisol in Southern Brazil. We assessed soil physical quality using the revised Peerlkamp technique and measured bulk density, air-filled porosity and air permeability of intact soil cores. Samples were incubated to assess in vitro N2O and CO2 production. The soil physical and structural properties showed consistent differences between interrow and row positions, where the properties measured were more favorable. The revised Peerlkamp technique proved as efficient as quantitative parameters in discriminating treatment differences. Overall, soil physical conditions in the interrow were less favourable than in the row. Pore continuity did not vary as regards position. This may explain why row position did not influence in vitro N2O and CO2 production. Soil physical quality under no-tillage system is enhanced, at least in the short term, by superficial disturbances in the row as a result of the action of the coulters of the no-tillage seeder.

  19. Effet d'un choc hypotonique sur le volume cellulaire et sur la concentration intracellulaire de calcium des cellules branchiales de truite arc-en-ciel

    Directory of Open Access Journals (Sweden)



    Full Text Available La branchie étant un organe à structure complexe, il était impossible d'étudier l'effet d'un choc hypotonique sur les cellules branchiales in situ. Une culture primaire de branchie de truite a donc été mise au point et l'effet d'une dilution du milieu extracellulaire sur ces cellules en culture a été étudié. Les cellules branchiales en culture primaire forment un épithélium à confluence après 5 à 7 jours de culture. Ces cellules présentent les caractéristiques morphologiques des cellules respiratoires de la branchie. Les mesures du volume cellulaire, lors d'un choc hypotonique, sont effectuées sur des populations isolées de cellules respiratoires, à l'aide du système Coulter counter. Lors de la dilution aux 2/3 du milieu externe, les cellules gonflent pour atteindre un volume moyen de 130% en 5 minutes. Ensuite, les cellules régulent lentement leur volume. Après 30 minutes dans les conditions hypotoniques, le gonflement cellulaire ne représente plus que 110% du volume initial. Lors d'un choc hypotonique, nous avons mesuré, à l'aide d'une sonde fluorescente sensible au calcium (le Fura-2, une augmentation de la concentration intracellulaire de calcium des cellules respiratoires en culture primaire.

  20. Distribution of In-111 in granulocyte and other cellular elements of blood (CEB) in human In-111-labeled mixed white cell (MWC) and platelet preparations

    International Nuclear Information System (INIS)

    Dewanjee, M.K.; Chowdhury, S.; Brown, M.L.; Wahner, H.W.


    A large number of platelets (PLT), red blood cells (RBC) are present along with granulocyte (GC) in In-111 in CEB was determined by Ficoll-Hypaque gradient (FHG) centrifugation of In-111-MWC and PLT preparation as a quality control procedure. MWC were separated by sedimentation with hydroxyethyl starch; PLT by differential centrifugation. MWC and PLT were labeled with In-111-oxine in saline, ACD-saline or with In-111-tropolone in 0.5 ml of ACD-plasma. 0.3-0.5 ml of labeled cell suspended in plasma was layered on 3 ml FHG of two densities (1.119 and 1.077 gm/ml) and spun in a clear polystyrene tube at 1800 G for 30 min. Four layers (plasma, PLT, GC, and RBC) were separated, and In-111 radioactivity in each fraction was determined with a gamma counter. Simultaneously cell types in MWC and PLT preparations were determined by Coulter counter and differential counting. Most of In-111 in In-MWC is associated with the PLT and RBC, GC/lymphocyte ratio is 6/4. GC has higher extraction efficiency than RBC and PLT. PLT preparation is pure and (96 +- 3)% of In-111 is bound to PLT, (4 +- 3)% to RBC and (0.2 +- 0.1)% to GC; PLT preparation contains PLT (97 +- 3)%, RBC (4 +- 3)% and GC (0.2 +- 0.1)%

  1. Effect on sport hemolysis of cold water leg immersion in athletes after training sessions. (United States)

    Banfi, Giuseppe; Melegati, Gianluca


    The principal source of increased turnover of erythrocytes in athletes is sport hemolysis, the intravascular hemolysis that characteristically occurs with athletic performance in sport. The use of the parameter mean sphered cell volume (MSCV), automatically measured by means of the Coulter LH750, could be useful for diagnosing the presence of sport hemolysis. We studied the behavior of MSCV and mean corpuscular volume (MCV) in 30 top-level rugby players who underwent a heavy training session followed by 3 different recovery methods, administered to 3 subgroups of 10 athletes. We tested the use of active recovery consisting of cold water (5 degrees C) immersion of legs for 10 minutes either before (n = 10) or after (n = 10) cycling at 180 W for 10 minutes. In the whole group of athletes, measurements performed at rest and after training session and recovery showed no differences in MCV and MSCV values. The difference between MCV and MSCV was significant in the whole group and in the subgroup performing passive recovery, whereas the difference was not significant in the subgroups performing active recovery. This finding indicates that the use of active recovery in the top-level rugby players prevented the modifications of erythrocyte volume and shape. We outline that the values of the difference between MCV and MSCV was significantly modified in the whole group but the variations were not significant in the active recovery subgroups. The use of an index of erythrocyte shape modification (MCV - MSCV) can be very useful for evaluating sport hemolysis.

  2. [Hemogram reference values: study of 1000 healthy adults from Sfax]. (United States)

    Ben Amor, I; Menif, H; Ben Hamida, A; Gdoura, I; Rekik, H; Gargouri, J


    The aims of this study is to determine the hemogram reference values in a population of healthy adults of the region of Sfax, to compare our results with those from the literature, to estimate the age and sex variations in the blood count. The hemogram parameters were analyzed in 1000 blood donors. Hemogram was performed using Coulter ACT10's analyser. The differential leukocyte count was manually performed. The means of erythrocyte count, hemoglobin, haematocrit and red cell indices were significantly lower in women than in men. There was a significant variation in the red blood cell count, hemoglobin and haematocrit with age. There is also sex and age differences of the leukocyte count. The neutrophil and eiosinophil counts were higher in women than in men. The lymphocytes decreased with age in both sexes. The platelet count was significantly higher in women than in men. These results can be applied only to our local population of adults. The definition of reference values for the Tunisian population requires a study on a more diversified population, including more important number of individuals from various regions of the country.

  3. Platelet storage at 22 degrees C: effect of type of agitation on morphology, viability, and function in vitro. (United States)

    Holme, S; Vaidja, K; Murphy, S


    Recovery in vivo after 51Cr labeling, platelet morphology, and platelet aggregation were studied with platelet concentrates (PC) stored for transfusion under carefully controlled conditions. PC were prepared to a final volume of 50 ml from whole blood anticoagulated with citrate-phosphate-dextrose (CPD). The platelet count was kept between 0.8 and 1.6 X 10(12) platelets/liter. The PC were stored in bags constructed of polyvinylchloride (PVC) or polyethylene (PE) at 22 degrees C for 72 hr. The bags were placed on a horizontal shaker or a ferris wheel for agitation during storage. No significant changes in pH or platelet count were observed during storage. PC stored on the wheel showed moderate loss of viability and a marked deterioration of platelet morphology and aggregation compared to the shaker. PC stored on the shaker in bags made of PE showed better aggregation with ADP and thrombin but had the same viability and morphology as PC in bags constructed of PVC. Maintenance of normal platelet morphology as determined by phase-contrast microscopy, extent of shape change response, and the size distribution according to the Coulter Counter correlated with recovery in vivo.

  4. Rice starch granule characterization by flow cytometry scattering techniques hyphenated with sedimentation field-flow fractionation. (United States)

    Clédat, Dominique; Battu, Serge; Mokrini, Redouane; Cardot, Philippe J P


    Sedimentation field-flow fractionation (SdFFF) elution mode of micron sized particle is described generically as "Hyperlayer" and involves particle size, density, shape and rigidity. It requires the use of specific detectors of mass, size, surface, or of other characteristics of the eluted particles. Correlation of FFF retention data with such signals gives hyphenated information about particle properties. Flow cytometry (FC) is a multi dimensional particle counter, which permits specific particle property characterization using light scattering and fluorescence principles. It appears therefore as a powerful technique for micron sized species description. FC is mostly known for cell analyses, while its potential is much broader once proper calibration performed. In this report, forward angle signal (FS) is calibrated in size by using standard latex beads and produces, for a given particle sample, a number versus size histogram, describing particle size distribution. These histograms can be an alternative to Coulter counting. That methodology is tested with rice starch population (RSP) fractions obtained from FFF separation.

  5. Nuclear plant shutdowns. Oversight Hearing before the Subcommittee on Energy and the Environment of the Committee on Interior and Insular Affairs, House of Representatives, Ninety-Sixth Congress, First Session, March 19, 1979

    International Nuclear Information System (INIS)



    Testifying individually at the one day hearings to consider the circumstances surrounding the Nuclear Regulatory Commission's (NRC) order that 5 nuclear powerplants be shut down pending analysis and possible modification of safety related piping systems were Henry Coulter, director, US Geological Survey; David F. Emery, US Representative, Maine; Daniel F. Ford, executive director, Union of Concerned Scientists; and Herbert E. Harris, US Representative, Virginia. Two panels also presented testimony. One of these consisted of members of the NRC, and the other was made up of representatives of utilities and construction firms active in the nuclear power industry directly influenced by the NRC order. The plants were ordered shut down because of an alleged faulty calculation made by Stone and Webster Engineering Corp. in determining whether certain types of systems in these plants could withstand earthquakes of differing degrees of magnitude or intensity. Social and economic costs of the shutdowns are considered at length. The closing statement of the chairman indicated that no real progress had been made after the day's testimony of deciding whether the shut down over a presumed safety problem was justified

  6. Relationship between Neutrophil-to-Lymphocyte Ratio and Inflammatory Markers in Sickle Cell Anaemia Patients with Proteinuria. (United States)

    Emokpae, Mathias Abiodun; Aruomaren, Austin; Osime, Evarista


    The renal functions and structure in sickle cell anaemia (SCA) patients may be affected by chronic haemodynamic changes and consequences of vaso-occlusive events in the renal medulla. Few reports on neutrophil-to-lymphocyte (NLR) and platelet-to-lymphocyte (PLR) ratios in SCA patients in Africans exist in the literature. This study correlates the values of NLR and PLR with measured traditional inflammatory markers in SCA patients with and without proteinuria and impaired kidney function (defined in this study as estimated glomerular filtration rate (eGFR), less than 60 mL/min/1.73 m². Full blood count, C-reactive protein (CRP), and fibrinogen were assayed in 150 SCA patients and 50 control subjects using Coulter Haematology analyser (CELL DYE 37000) and ELISA method, respectively. The NLR and PLR were calculated by dividing absolute neutrophil or platelet counts by absolute lymphocyte count. Fibrinogen, CRP, NLR, and PLR increased progressively ( p proteinuria, with the highest values seen in those with impaired renal function. NLR correlated positively with CRP and fibrinogen in SCA patients without proteinuria ( p proteinuria ( p < 0.001), and impaired renal function ( p < 0.05). A positive relationship was also observed between NLR and fibrinogen in the control subjects. The need to determine cut-off values for these leukocyte ratios to be used in identifying those patients at risk and in the general management of SCA patients is suggested.

  7. Multi Source Remote Sensing for Monitoring Light-Absorbing Impurities on Snow and Ice in the European Alps (United States)

    Colombo, R.; Baccolo, G.; Garzonio, R.; Massabò, D.; Julitta, T.; Rossini, M.; Ferrero, L.; Delmonte, B.; Maggi, V.; Mattavelli, M.; Panigada, C.; Cogliati, S.; Cremonese, E.; Di Mauro, B.


    The European Alps are located close to one of the most industrialized areas of the planet and they are 3.000 km from the largest desert of the Earth. Light-absorbing impurities (LAI) emitted from these sources can reach the Alpine chain and deposit on snow covered areas and mountain glaciers. Although several studies show that LAI have important impacts on the optical properties of snow and ice, reducing the albedo and promoting the melt, this impact has been poorly characterized in the Alps. In this contribution, we present the results of a multisource remote sensing approach aimed to study the LAI impact on snow and ice properties in the Alpine area. This process has been observed by means of remote and proximal sensing methods, using satellite (Landsat 8, Hyperion and MODIS data), field spectroscopy (ASD measurements), Automatic Weather Stations, aerial surveys (Unmanned Aerial Vehicle), radiative transfer modeling (SNICAR and TARTES) and laboratory analysis (hyperspectral imaging system). Furthermore, particle size (Coulter Counter), geochemical (Instrumental Neutron Activation Analysis, INAA) and optical (Multi-Wavelength Absorbance Analyzer, MWAA) analyses have been applied to determine the nature and radiative properties of particulate material deposited on snow and ice or aggregated into cryoconite holes. Our results demonstrate that LAI can be monitored from remote sensing at different scale. LAI showed to have a strong impact on the Alpine cryosphere, paving the way for the assessment of their role in melting processes.

  8. Premarital Screening of Beta Thalassemia Minor in north-east of Iran (United States)

    Hashemizadeh, H; Noori, R


    Background Beta thalassemia is a preventable disease. Iran has about 20,000Patients who are homozygote for β-thalassaemia and 3,750,000 carriers. The aim of this study was to determine the prevalence of beta thalassemia minor among men who underwent premarital screening in Quchana city in Khorasan Razavi region of Iran Materials and Methods This research is a descriptive cross-sectional study. From 2010 to 2011, all participants (1000) under marriage coming to health center of Quchan underwent routine mandatory tests. Participants were considered to have beta-thalassemia minor on the condition that hey had a mean corpuscular volume (MCV) 3.5%. Venous blood was taken into an EDTA tube and the complete blood count and red blood cell indices were measured with a Coulter automated cell counter. Electrophoresis was performed on cellulose acetate. Results Mean and SD of hemoglobin, MCV and MCH were 16±2.9, 91±4 and 28.4±2, respectively. Hemoglobin A2 Higher than 3.5 percent was reported as 3.5%.The prevalence of beta-thassemia minor with high hemoglobin A2 and microcytic hypochromic anemia was 3.5% (P-value). Conclusion In countries with high prevalence of hemoglobinopathies, a premarital screening program is helpful for identification and prevention of high-risk marriages. Detecting carrier couples with premarital screening program is an effective way of controlling thalassemia major. PMID:24575266

  9. Prehistoric peyote use: alkaloid analysis and radiocarbon dating of archaeological specimens of Lophophora from Texas. (United States)

    El-Seedi, Hesham R; De Smet, Peter A G M; Beck, Olof; Possnert, Göran; Bruhn, Jan G


    Two archaeological specimens of peyote buttons, i.e. dried tops of the cactus Lophophora williamsii (Lem.) Coulter, from the collection of the Witte Museum in San Antonio, was subjected to radiocarbon dating and alkaloid analysis. The samples were presumably found in Shumla Cave No. 5 on the Rio Grande, Texas. Radiocarbon dating shows that the calibrated 14C age of the weighted mean of the two individual dated samples corresponds to the calendric time interval 3780-3660 BC (one sigma significance). Alkaloid extraction yielded approximately 2% of alkaloids. Analysis with thin-layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS) led to the identification of mescaline in both samples. No other peyote alkaloids could be identified. The two peyote samples appear to be the oldest plant drug ever to yield a major bioactive compound upon chemical analysis. The identification of mescaline strengthens the evidence that native North Americans recognized the psychotropic properties of peyote as long as 5700 years ago.

  10. How does the greater white-toothed shrew, Crocidura russula, responds to long-term heavy metal contamination? - A case study

    International Nuclear Information System (INIS)

    Marques, Carla Cristina; Sanchez-Chardi, Alejandro; Gabriel, Sofia Isabel; Nadal, Jacint; Viegas-Crespo, Ana Maria; Luz Mathias, Maria da


    Heavy metals accumulation in parallel with the evaluation of physiological and biochemical effects resulting from continued metal exposure were considered here using for the first time the great white-toothed shrew Crocidura russula as an in vivo model. Shrews were originated from an abandoned lead/zinc mining area and from a reference area, both in Alentejo, southern Portugal. Hepatic contents of nickel, copper, zinc, cadmium, mercury and lead were quantified by Inductively Coupled Plasma Mass Spectrometer (ICP-MS). Haematological parameters (white blood cells, red blood cells, haemoglobin and haematocrit) were obtained in a Coulter Counter Analyser and biochemical markers of the redox balance (glutathione S-transferase, glutathione peroxidase, and glutathione reductase) activities were measured spectrophotometrically using a Duo-50 spectrophotometer. Compared with control animals, significantly higher concentration of hepatic cadmium (9.29 vs. 1.18 μg/g dry weight) and nickel (1.56 vs. 0.343 μg/g dry weight) were detected in the shrews collected in the mining area. However, no significant changes were observed on haematological or enzymatic parameters in animals exposed to metal pollution. The obtained results show that shrews are good bioaccumulators of toxic heavy metals, but very tolerant to their effects, revealing an interesting long-term adaptation to polluted environments. In addition, this study provides reference values for haematological parameters and antioxidant enzymes levels in C. russula, which may be relevant for comparative purposes in further studies

  11. Root growth conditions in the topsoil as affected by tillage intensity

    DEFF Research Database (Denmark)

    Kadziene, Grazina; Munkholm, Lars Juhl; Mutegi, James


    in the topsoil. Samples were taken from a 7-year tillage experiment on a Danish sandy loam at Foulum, Denmark (56°30′ N, 9°35′ E) in 2008. The main crop was spring barley followed by either dyer's woad (Isatis tinctoria L.) or fodder radish (Raphanus sativus L.) cover crops as subtreatment. The tillage...... treatments were direct drilling (D), harrowing 8–10 cm (H), and ploughing (P) to 20 cm depth. A chisel coulter drill was used in the H and D treatments and a traditional seed drill in the P treatment. Undisturbed soil cores were collected in November 2008 at soil field moisture capacity from the 4–8 and 12....... Fodder radish resulted in more macropores, higher gas diffusivity and lower pore tortuosity compared to dyer's woad. This was especially important for the H treatment where compaction was a significant problem at the lower depths of the arable layer (10–20 cm depth). Our results suggest that fodder...

  12. Mineral Analysis of Pine Nuts (Pinus spp. Grown in New Zealand

    Directory of Open Access Journals (Sweden)

    Leo P. Vanhanen


    Full Text Available Mineral analysis of seven Pinus species grown in different regions of New Zealand; Armand pine (Pinus armandii Franch, Swiss stone pine (Pinus cembra L., Mexican pinyon (Pinus cembroides Zucc. var. bicolor Little, Coulter pine (Pinus coulteri D. Don, Johann’s pine (Pinus johannis M.F. Robert, Italian stone pine (Pinus pinea L. and Torrey pine (Pinus torreyana Parry ex Carrière, was carried out using an inductively coupled plasma optical emission spectrophotometer (ICP-OES analysis. Fourteen different minerals (Al, B, Ca, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, S and Zn were identified in all seven varieties, except that no Al or Na was found in Pinus coulteri D. Don. New Zealand grown pine nuts are a good source of Cu, Mg, Mn, P and Zn, meeting or exceeding the recommended RDI for these minerals (based on an intake of 50 g nuts/day while they supplied between 39%–89% of the New Zealand RDI for Fe. Compared to other commonly eaten tree-nuts New Zealand grown pine nuts are an excellent source of essential minerals.

  13. Mineral Analysis of Pine Nuts (Pinus spp.) Grown in New Zealand (United States)

    Vanhanen, Leo P.; Savage, Geoffrey P.


    Mineral analysis of seven Pinus species grown in different regions of New Zealand; Armand pine (Pinus armandii Franch), Swiss stone pine (Pinus cembra L.), Mexican pinyon (Pinus cembroides Zucc. var. bicolor Little), Coulter pine (Pinus coulteri D. Don), Johann’s pine (Pinus johannis M.F. Robert), Italian stone pine (Pinus pinea L.) and Torrey pine (Pinus torreyana Parry ex Carrière), was carried out using an inductively coupled plasma optical emission spectrophotometer (ICP-OES) analysis. Fourteen different minerals (Al, B, Ca, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, S and Zn) were identified in all seven varieties, except that no Al or Na was found in Pinus coulteri D. Don. New Zealand grown pine nuts are a good source of Cu, Mg, Mn, P and Zn, meeting or exceeding the recommended RDI for these minerals (based on an intake of 50 g nuts/day) while they supplied between 39%–89% of the New Zealand RDI for Fe. Compared to other commonly eaten tree-nuts New Zealand grown pine nuts are an excellent source of essential minerals. PMID:28239104

  14. Mineral Analysis of Pine Nuts (Pinusspp.) Grown in New Zealand. (United States)

    Vanhanen, Leo P; Savage, Geoffrey P


    Mineral analysis of seven Pinus species grown in different regions of New Zealand; Armand pine ( Pinus armandii Franch), Swiss stone pine ( Pinus cembra L.), Mexican pinyon ( Pinus cembroides Zucc. var. bicolor Little), Coulter pine ( Pinus coulteri D. Don), Johann's pine ( Pinus johannis M.F. Robert), Italian stone pine ( Pinus pinea L.) and Torrey pine ( Pinus torreyana Parry ex Carrière), was carried out using an inductively coupled plasma optical emission spectrophotometer (ICP-OES) analysis. Fourteen different minerals (Al, B, Ca, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, S and Zn) were identified in all seven varieties, except that no Al or Na was found in Pinus coulteri D. Don. New Zealand grown pine nuts are a good source of Cu, Mg, Mn, P and Zn, meeting or exceeding the recommended RDI for these minerals (based on an intake of 50 g nuts/day) while they supplied between 39%-89% of the New Zealand RDI for Fe. Compared to other commonly eaten tree-nuts New Zealand grown pine nuts are an excellent source of essential minerals.

  15. Regrowth and radiation sensitivity of quiescent cells isolated from EMT6/Ro-fed plateau monolayers

    International Nuclear Information System (INIS)

    Luk, C.K.; Keng, P.C.; Sutherland, R.M.


    A quiescent [denoted as Q(G0/G1)] subpopulation was isolated from EMT6/Ro-fed plateau monolayers by centrifugal elutriation. The median Coulter volume of these cells was significantly smaller than that of the original population from which they were elutriated. Using two-step acridine orange staining and dual parameter flow cytometric analysis, over 95% of quiescent cells were found to have G1 DNA content, and 80% of the cells had a decreased RNA content as compared to rapidly proliferating exponential G1 cells. After labeling for 24 hr (two doubling times) with [ 3 H]thymidine, less than 2% of the quiescent cells incorporated [3H]thymidine as measured by autoradiography. The colony-forming efficiency of these cells was not significantly different from that of exponential cells. When such Q(G0/G1) cells were replated in fresh medium at a lower density, there was a lag time of 30 hr before any increase in cell number was detected, after which the cell-doubling rate matched that of exponential culture. Results obtained from the radiation dose-response curves showed that quiescent (G0/G1) cells were more radiosensitive than exponential G1 or unseparated fed plateau cells

  16. Cytotoxic effect of the pentacyclic oxindole alkaloid mitraphylline isolated from Uncaria tomentosa bark on human Ewing's sarcoma and breast cancer cell lines. (United States)

    García Giménez, Dolores; García Prado, Elena; Sáenz Rodríguez, Teresa; Fernández Arche, Angeles; De la Puerta, Rocío


    Preparations from Uncaria tomentosa, a South American Rubiaceae, have been used in the Peruvian traditional medicine for the treatment of infective, inflammatory and tumoral processes. In this study, the pentacyclic oxindole alkaloid mitraphylline was isolated from the dried inner bark of this plant species, and its structure elucidated by analysis of NMR spectroscopic data. Mitraphylline was differentially identified from its stereoisomeric pair isomitraphylline by (15)N-NMR. Its antiproliferative and cytotoxic effects have been tested on human Ewing's sarcoma MHH-ES-1 and breast cancer MT-3 cell lines, using cyclophosphamide and vincristine as reference controls. A Coulter counter was used to determine viable cell numbers, followed by the application of the tetrazolium compound MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy phenyl)-2-(4-sulfophenyl)-2H-tetrazolium] an inner salt. A colorimetric method was employed to evaluate cell viability in this cytotoxic assay. Micromolar concentrations of mitraphylline (5 microM to 40 microM) inhibited the growth of both cell lines in a dose-dependent manner. The IC (50) +/- SE values were 17.15 +/- 0.82 microM for MHH-ES-1 and 11.80 +/- 1.03 microM for MT-3 for 30 hours, smaller than those obtained for the reference compounds. This action suggests that the pentacyclic oxindole alkaloid mitraphylline might be a new promising agent in the treatment of both human sarcoma and breast cancer. Georg Thieme Verlag KG Stuttgart . New York.

  17. SR140333 counteracts NK-1 mediated cell proliferation in human breast cancer cell line T47D

    Directory of Open Access Journals (Sweden)

    Wei Hong-Jun


    Full Text Available Abstract Background It has been demonstrated that certain NK-1 antagonists could reduce proliferation of several cancer cell lines, however, it is unknown whether SR140333 exerts proliferation inhibition in breast cancer cell line. Methods Immunohistochemical staining was carried out to investigate the immunolocation of NK-1 in breast cancer tissues and T47D cell line, thereafter, various concentrations of [Sar9, Met(O211]substance P and SR140333 were applied alone or combined. MTT assay was applied to detect cytoactivation and coulter counter was to detect growth curve. The Hoechst33258 staining was performed to detect apoptosis. Results We found that breast cancer and T47D cells bear positive expression of NK-1. SR140333 inhibited cell growth in a dose dependent manner. Furthermore, SR140333 could counteract [Sar9, Met(O211]substance P induced proliferation. Hoechst33258 staining revealed the presence of apoptosis after SR140333 treatment. Conclusions Our study demonstrated SR140333 exert proliferation inhibition in breast cancer cell line T47D and indicates NK-1 play a central role in the substance P related cell proliferation in breast cancer.

  18. SR140333 counteracts NK-1 mediated cell proliferation in human breast cancer cell line T47D. (United States)

    Huang, Wei-Qing; Wang, Ji-Gang; Chen, Lei; Wei, Hong-Jun; Chen, Hua


    It has been demonstrated that certain NK-1 antagonists could reduce proliferation of several cancer cell lines, however, it is unknown whether SR140333 exerts proliferation inhibition in breast cancer cell line. Immunohistochemical staining was carried out to investigate the immunolocation of NK-1 in breast cancer tissues and T47D cell line, thereafter, various concentrations of [Sar9, Met(O2)11]substance P and SR140333 were applied alone or combined. MTT assay was applied to detect cytoactivation and coulter counter was to detect growth curve. The Hoechst33258 staining was performed to detect apoptosis. We found that breast cancer and T47D cells bear positive expression of NK-1. SR140333 inhibited cell growth in a dose dependent manner. Furthermore, SR140333 could counteract [Sar9, Met(O2)11]substance P induced proliferation. Hoechst33258 staining revealed the presence of apoptosis after SR140333 treatment. Our study demonstrated SR140333 exert proliferation inhibition in breast cancer cell line T47D and indicates NK-1 play a central role in the substance P related cell proliferation in breast cancer.

  19. Development of tissue-culture methods for the in-vitro evaluation of polysaccharide wound management products

    Energy Technology Data Exchange (ETDEWEB)

    Spyratou, L.


    The use of normal human fibroblasts, mouse connective tissue fibroblasts (L929), and guinea-pig epidermal cells in culture, as possible systems for evaluating wound management products was investigated. This necessitated the development of a biological assay system for comparing the effects of wound management products on the skin cell lines. Polysaccharide based wound management products were selected for this study. Published tissue-culture assay methods were found to be inadequate, hence a new biological assay system was developed and validated. The haemacytometer chamber, the Coulter counter and the incorporation of /sup 3/(H)-thymidine as methods for assessing growth were compared. It was found that final cell growth in control culture was dependent on the frequency of change of media, on substrate area, and on initial cell number, which at confluency was found to be related to the final yield of cells by equation y = 1420 * 10/sup (/minus/0.004x)/. Representative monosaccharides and polysaccharides were tested in addition to the wound management products.

  20. Vascular volumes and hematology in male and female runners and cyclists. (United States)

    Green, H J; Carter, S; Grant, S; Tupling, R; Coates, G; Ali, M


    To examine the hypothesis that foot-strike hemolysis alters vascular volumes and selected hematological properties is trained athletes, we have measured total blood volume (TBV), red cell volume (RCV) and plasma volume (PV) in cyclists (n = 21) and runners (n = 17) and compared them to those of untrained controls (n = 20). TBV (ml x kg(-1)) was calculated as the sum of RCV (ml x kg(-1)) and PV (ml x kg(-1)) obtained using 51Cr and 125I-labelled albumin, respectively. Hematological assessment was carried out using a Coulter counter. Peak aerobic power (VO2peak) was measured during progressive exercise to fatigue using both cycle and treadmill ergometry. RCV was 15% higher (P strike hemolysis would not appear to have an effect on that parameter. The significant correlations (P < 0.05) found between VO2peak and RCV (r = 0.64 and 0.64) and TBV (r = 0.82 and 0.63) for the males and females, respectively, suggests a role for the vascular system in realizing a high aerobic power.

  1. Nanopore sequencing detects structural variants in cancer. (United States)

    Norris, Alexis L; Workman, Rachael E; Fan, Yunfan; Eshleman, James R; Timp, Winston


    Despite advances in sequencing, structural variants (SVs) remain difficult to reliably detect due to the short read length (nanopore sequencing on the MinION. Nanopore sequencing relies on a similar concept to a Coulter counter, reading the DNA sequence from the change in electrical current resulting from a DNA strand being forced through a nanometer-sized pore embedded in a membrane. Though nanopore sequencing currently has a relatively high mismatch rate that precludes base substitution and small frameshift mutation detection, its accuracy is sufficient for SV detection because of its long reads. In fact, long reads in some cases may improve SV detection efficiency. We have tested nanopore sequencing to detect a series of well-characterized SVs, including large deletions, inversions, and translocations that inactivate the CDKN2A/p16 and SMAD4/DPC4 tumor suppressor genes in pancreatic cancer. Using PCR amplicon mixes, we have demonstrated that nanopore sequencing can detect large deletions, translocations and inversions at dilutions as low as 1:100, with as few as 500 reads per sample. Given the speed, small footprint, and low capital cost, nanopore sequencing could become the ideal tool for the low-level detection of cancer-associated SVs needed for molecular relapse, early detection, or therapeutic monitoring.

  2. Prevalence of anemia and deficiencies of iron, folic acid and vitamin B12 in an indigenous community from the Venezuelan Amazon with a high incidence of malaria. (United States)

    García-Casal, Maria Nieves; Leets, Irene; Bracho, Carmen; Hidalgo, Mariana; Bastidas, Gilberto; Gomez, Ana; Peña, Ana; Pérez, Hilda


    The objective of this work was to determine the prevalence of anemia and deficiencies of iron, folic acid and vitamin B12 in Betania del Topocho, a Piaroa community from Estado Amazonas, Venezuela, a zone with a high incidence of malaria. The group studied included 184 subjects of all ages that assisted to the local health center for malaria diagnosis. Analysis performed included hematology by coulter counter, ferritin quantification by ELISA with monoclonal antibodies and folic acid and vitamin B12 determinations by an immunoradiometric assay. It was found that the prevalence of anemia was 89.6% and deficiencies of iron, folic acid and vitamin B12 affected 37.1,70.3 and 12.4% of the population studied, respectively. Plasmodium infection was detected by molecular diagnosis in 53.2% of the cases, and 86% of them were anemic. The highest incidence of anemia was found in children, with a prevalence of 100% in infants of both sexes. The high prevalence of anemia, iron and folic acid deficiencies found, indicates an important health and nutrition problem that should be immediately and properly addressed. The number of cases of anemia due to iron deficiency could be underestimated, since ferritin concentration increased as a acute phase protein, although prevalence data was also analyzed with a cutoff point of 30 microg/L for ferritin concentration.

  3. Proxies and measurement techniques for mineral dust in Antarctic ice cores. (United States)

    Ruth, Urs; Barbante, Carlo; Bigler, Matthias; Delmonte, Barbara; Fischer, Hubertus; Gabrielli, Paolo; Gaspari, Vania; Kaufmann, Patrik; Lambert, Fabrice; Maggi, Valter; Marino, Federica; Petit, Jean-Robert; Udisti, Roberto; Wagenbach, Dietmar; Wegner, Anna; Wolff, Eric W


    To improve quantitative interpretation of ice core aeolian dust records, a systematic methodological comparison was made. This involved methods for water-insoluble particle counting (Coulter counter and laser-sensing particle detector), soluble ion analysis (ion chromatography and continuous flow analysis), elemental analysis (inductively coupled plasma mass spectroscopy at pH 1 and after full acid digestion), and water-insoluble elemental analysis (proton induced X-ray emission). Antarctic ice core samples covering the last deglaciation from the EPICA Dome C (EDC) and the EPICA Dronning Maud Land (EDML) cores were used. All methods correlate very well among each other, but the ratios of glacial age to Holocene concentrations, which are typically a factor approximately 100, differ between the methods by up to a factor of 2 with insoluble particles showing the largest variability. The recovery of ICP-MS measurements depends on the digestion method and is differentfor different elements and during different climatic periods. EDC and EDML samples have similar dust composition, which suggests a common dust source or a common mixture of sources for the two sites. The analyzed samples further reveal a change of dust composition during the last deglaciation.

  4. Using Failure Mode and Effect Analysis (FMEA for Performing Good Ploughing with Mouldboard

    Directory of Open Access Journals (Sweden)

    M Namdari


    Full Text Available Farm management needs creative methods to success. FMEA (Failure Modes and Effects Analysis is a new method to analyze potential reliability problems in the development cycle of the project, making it easier to take actions to overcome such issues, thus enhancing the reliability through design or process. Anticipating these failure modes, being the central step in the analysis, needs to be carried on extensively, in order to prepare a list of maximum potential failure modes. Risk is measured in terms of Risk Priority Number (RPN that is a product of occurrence, severity, and detection difficulty. This study attempted to improve clod mean weight diameter and soil inversion as indicators of tillage quality by FMEA methodology. The results showed that low soil moisture, slow speed of ploughing and great depth of ploughing is the most important factors that increase clod MWD with 900, 630 and 560 RPN, respectively. Also for soil inversion the slow speed of ploughing, not using coulter, low soil moisture and great depth of ploughing are important factors with 720, 648, 490 and 420 RPN. Using a split - split factorial experiment with 16 treatments and three replications also acknowledged the results of this method. After reforming the conditions and re-testing the experiment, results showed that clod MWD was reduced 20% and soil inversion increased 2% approximately. This study proposes the use of this technique in agricultural management.

  5. Infection-stimulated anemia results primarily from interferon gamma-dependent, signal transducer and activator of transcription 1-independent red cell loss. (United States)

    Wang, Zheng; Zhang, Dong-Xia; Zhao, Qi


    Although the onset of anemia during infectious disease is commonly correlated with production of inflammatory cytokines, the mechanisms by which cytokines induce anemia are poorly defined. This study focused on the mechanism research. Different types of mice were infected perorally with Toxoplasma gondii strain ME49. At the indicated times, samples from each mouse were harvested, processed, and analyzed individually. Blood samples were analyzed using a Coulter Counter and red blood cell (RBC) survival was measured by biotinylation. Levels of tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), and inducible protein 10 (IP-10) mRNA in liver tissue were measured by real-time polymerase chain reaction. T. gondii-infected mice exhibited anemia due to a decrease in both erythropoiesis and survival time of RBC in the circulation (P anemia was associated with fecal occult, supporting previous literature that hemorrhage is a consequence of T. gondii infection in mice. Infection-induced anemia was abolished in interferon gamma (IFNγ) and IFNγ receptor deficient mice (P anemia resulting solely from increased loss of circulating RBC. Infection-stimulated decrease in erythropoiesis and losses of RBC have distinct mechanistic bases. These results show that during T. gondii infection, IFNγ is responsible for an anemia that results from both a decrease in erythropoiesis and a STAT1 independent loss of circulating RBC.

  6. Lamellar bodies: platelet channel particles as predictors of respiratory distress syndrome (RDS) and of transient tachypnea of the newborn. (United States)

    Piazze, Juan; Cerekja, Albana


    We aimed to find a single numeric cut-off point in order to predict respiratory distress syndrome (RDS) and transient tachypnea of the newborn (TTN), through quantification of the lamellar bodies (LBs) count. Two hundred and twenty-seven single pregnancies delivered within 48 h from amniocentesis in a III level university hospital in a period between 1996 and 2007. LBs counts were determined by amniotic fluid (AF) sampling and after centrifugation in a commercially available Coulter counter. LBs counts were performed in order to predict the risk of neonatal RDS and also evaluated against the risk of TTN by means of the most suitable receiver operator characteristic (ROC) curve in order to predict RDS and TTN. The best LBs cut-off to significantly predict TTN was ≤ 35,000/μL [sensitivity 86%, specificity 75%, positive predictive value (PPV) 22% and negative predictive value (NPV) at 96%]. An LBs count ≤ 32,000/μL was the best compromise between sensitivity and specificity in predicting RDS or TTN [sensitivity 86%, specificity 83%, PPV 37% and NPV at 97%]. The quantification of LBs is a reliable method, simple to execute, useful in predicting RDS, and may be useful in evaluating suspicion of TTN.

  7. Microfluidic Transducer for Detecting Nanomechanical Movements of Bacteria (United States)

    Kara, Vural; Ekinci, Kamil


    Various nanomechanical movements of bacteria are currently being explored as an indication of bacterial viability. Most notably, these movements have been observed to subside rapidly and dramatically when the bacteria are exposed to an effective antibiotic. This suggests that monitoring bacterial movements, if performed with high fidelity, can offer a path to various clinical microbiological applications, including antibiotic susceptibility tests. Here, we introduce a robust and sensitive microfluidic transduction technique for detecting the nanomechanical movements of bacteria. The technique is based on measuring the electrical fluctuations in a microchannel which the bacteria populate. These electrical fluctuations are caused by the swimming of motile, planktonic bacteria and random oscillations of surface-immobilized bacteria. The technique provides enough sensitivity to detect even the slightest movements of a single cell and lends itself to smooth integration with other microfluidic methods and devices; it may eventually be used for rapid antibiotic susceptibility testing. We acknowledge support from Boston University Office of Technology Development, Boston University College of Engineering, NIH (1R03AI126168-01) and The Wallace H. Coulter Foundation.

  8. Transcellular ion flow in Escherichia coli B and electrical sizing of bacterias. (United States)

    Zimmermann, U; Schulz, J; Pilwat, G


    Dielectric breakdown of cell membranes and, in response, transcellular ion flows were measured in Escherichia coli B 163 and B 525 using a Coulter counter as the detector with a hydrodynamic jet focusing close to the orifice of the counter. Plotting the relative pulse height for compensated amplification of a certain size of the cells against increasing detector current, a rather sharp bend within the linear function was found, which did not occur when measuring fixed cells or polystyrene latex. The start current for transcellular ion flow causing the change of the slope is different for the potassium-deficient mutant B 525 in comparison with the wild-type B 163, indicating a change in the membrane structure of B 525 by mutation and demonstrating the sensitivity of the method for studying slight changes in membrane structure in general. The theoretical size distributions for two current values in the range of transcellular ion flow were constructed from the true size distribution at low detector currents, assuming an idealized sharp changeover of the bacterial conductivity from zero to one-third of the electrolyte conductivity.

  9. Preparo de hidrolisados protéicos para a análise de aminoácidos Preparation of protein hydrolysates for amino acid analysis

    Directory of Open Access Journals (Sweden)

    Carlos Roberto Bernardi


    Full Text Available Amostras de milho, farelo de soja e hidrolisado ácido de caseína foram submetidas à hidrólise ácida em estufa (110 ± 5°C, durante 22horas com ácido clorídrico 6 N, em ampolas de vidro seladas sob vácuo. Os hidrolisados foram preparados por quatro métodos: 1 evaporação em evaporador rotativo sob vácuo; 2 evaporação em câmaras a vácuo em presença de pastilhas de NaOH; 3 evaporação em bloco de aquecimento sob fluxo de argônio; 4 neutralização com solução padrão de citrato/NaOH. Os aminoácidos foram separados e quantificados em analisador específico Beckman System 6300 com derivatização pós-coluna por ninidrina. O método de neutralização dos reagentes da hidrólise apresentou os melhores resultados considerando todos os aminoácidos. Além disso, foi mais rápido e prático no preparo de um grande número de amostras. Quanto aos métodos de evaporação, o uso de evaporador rotativo apresentou perdas significativas apenas para treonina e serina na amostra farelo de soja; sob fluxo de argônio observou-se baixos teores para treonina, fenilalanina e lisina; em câmara sob vácuo verificou-se perdas significativas para treonina, serina e tirosina.Samples of corn, soybean corn and casein (acid hydrolysate were submitted to acid hydrolysis in oven (110 ± 5°C, for 22 hours with 6 N HCl in sealed ampoules under vacuum. Four methods of hydrolysate preparation were used: 1 evaporation to dryness in rotary evaporator under vacuum, 2 evacuated desiccator under vacuum and NaOH, 3 flushing the open vial with argon and 4 neutralisation with standard citrate/NaOH solution. Amino acids were isolated and quantified in specific analyzer Beckman System 6300 with ninhidrin post-column derivatization. The neutralization method presented the best result considering all analysed amino acids. Besides, it was more practical for rapid processing of a large number of samples. With regard to the evaporation methods in the rotary evaporator

  10. Phytoremediation of disturbed lands in Polar Regions of Russia (United States)

    Shamsutdinova, Evgeniya


    In the Northern regions the problem of restoration of disturbed lands as a result of anthropogenic activities is particularly acut. A large role for the success of native plants to take hold and re-establish themselves is to allow the natural process to work without human intervention. However observation shows that low reserves of available moisture, poor soil nutrients, exposure to wind and water erosion make impossible of a complete restoration of lands due to natural revegetation. One of the methods used to minimize the effect of these factors is the phytoremediation. Research on the Pelyatinskom gas condensate field in the Taimyr Dolgano-Nenets municipal district revealed the efficacy of biological remediation of awnless brome, wildrye siberian, red fescue and kentucky bluegrass. Geobotanical studies of the tailings fields in Yakutia showed that for the phytoremediation of tailings of alluvial deposits of diamonds slough grass can be successfully used, and on the dumps of coal deposits with a high degree of survival of the willow, poplar, larch, alder stand and pine. Development of technology for remediation of placer gold deposits of the Komsomol mine and Bilibino in Chukotka has shown the effectiveness of sowing common oat, wildrye and larch. The study of the experience of recultivation on objects of the "Transneft-Baltic" in the North-Western Federal district allowed us to select as the recommended mixtures of species: meadow fescue, cocksfoot, timothy grass, white and alsike clover and alfalfa. The multicomponent mixture of red fescue, awnless brome, meadow fescue, timothy grass, couch grass, kentucky bluegrass, beckman's grass were successfully used at the mine of the Bovanenkovo oil and gas condensate field. On the technological grounds of the complex pumping station in the Yamalo-Nenets Autonomous area willows were planted. Pine and willow trees were planted on quarry workings in the Northern taiga of Western Siberia land .Planting of pines has shown

  11. Scientific Analysis Cover Sheet for Radionuclide Screening

    Energy Technology Data Exchange (ETDEWEB)

    G. Ragan


    pressurized water reactor (PWR) fuel, U.S. Department of Energy (DOE) spent nuclear fuel (DSNF), and high-level waste (HLW). Average and outlying (high burnup, high initial enrichment, low age, or otherwise exceptional) forms of each waste-form type are considered. This analysis has been prepared in accordance with a technical work plan (BSC 2002c). In a review of Revision 00 of this radionuclide screening analysis, the NRC found that ''processes that affect transport in the biosphere, such as uptake by plants and bioaccumulation are not accounted for'' and that ''the direct exposure pathway is not accounted for'' (Beckman 2001, Section The NRC also found that the solubility and sorption classes were too broadly defined, noting, for example, that Se is in the same solubility and sorptivity groups as Np and U, yet is ''more soluble than Np and U by several orders of magnitude'' (Beckman 2001, Section This revision seeks to build upon the strengths of the earlier screening method while responding to the specific concerns raised by the NRC and other reviewers. In place of simple inhalation and ingestion dose conversion factors, the revised radionuclide screening uses screening factors that also take into account soil accumulation, uptake by plants, exposure to contaminated ground, and other features of the biosphere that were neglected in the previous screening. Whereas the previous screening analysis allowed only two solubility classes (soluble and insoluble), the revised screening introduces an intermediate solubility class to better segregate the radionuclides into transport groups.

  12. Kearns–Sayre syndrome: a case series of 35 adults and children

    Directory of Open Access Journals (Sweden)

    Khambatta S


    Full Text Available Sherezade Khambatta, Douglas L Nguyen, Thomas J Beckman, Christopher M Wittich Division of General Internal Medicine, Mayo Clinic, Rochester, MN, USA Background: Kearns–Sayre syndrome (KSS is a rare mitochondrial cytopathy, first described at Mayo Clinic in 1958. Aims: We aimed to define patient and disease characteristics in a large group of adult and pediatric patients with KSS. Methods: We retrospectively searched the Mayo Clinic medical index patient database for the records of patients with KSS between 1976 and 2009. The 35 patients identified with KSS were analyzed in terms of demographic characteristics, presenting signs and symptoms, diagnostic features, clinical evolution, and associations between disease features and the development of disability. Results: The mean (standard [SD] age at KSS presentation was 17 (10 years, but the mean age at diagnosis was 26 (15 years. Ophthalmologic symptoms developed in all patients, and neurologic and cardiac involvement was common. Only four patients (11% in the series died, but all deaths were from sudden cardiac events. The development of physical disability was significantly associated with cognitive decline (P=0.004 but not with other clinical features, such as sex or sudden cardiac death. Conclusion: We report the largest case series to date of patients with KSS from a single institution. In addition to the conduction system abnormalities identified in previous series, our cohort included patients with syncope and sudden cardiac death. This underscores the need to consider formal electrophysiologic studies and prophylactic defibrillators in patients with KSS. Keywords: heart block, mitochondrial diseases, ophthalmoplegia, retinitis pigmentosa

  13. Stop consonant voicing in young children's speech: Evidence from a cross-sectional study (United States)

    Ganser, Emily

    There are intuitive reasons to believe that speech-sound acquisition and language acquisition should be related in development. Surprisingly, only recently has research begun to parse just how the two might be related. This study investigated possible correlations between speech-sound acquisition and language acquisition, as part of a large-scale, longitudinal study of the relationship between different types of phonological development and vocabulary growth in the preschool years. Productions of voiced and voiceless stop-initial words were recorded from 96 children aged 28-39 months. Voice Onset Time (VOT, in ms) for each token context was calculated. A mixed-model logistic regression was calculated which predicted whether the sound was intended to be voiced or voiceless based on its VOT. This model estimated the slopes of the logistic function for each child. This slope was referred to as Robustness of Contrast (based on Holliday, Reidy, Beckman, and Edwards, 2015), defined as being the degree of categorical differentiation between the production of two speech sounds or classes of sounds, in this case, voiced and voiceless stops. Results showed a wide range of slopes for individual children, suggesting that slope-derived Robustness of Contrast could be a viable means of measuring a child's acquisition of the voicing contrast. Robustness of Contrast was then compared to traditional measures of speech and language skills to investigate whether there was any correlation between the production of stop voicing and broader measures of speech and language development. The Robustness of Contrast measure was found to correlate with all individual measures of speech and language, suggesting that it might indeed be predictive of later language skills.

  14. Evaluation of a third party enzymatic ammonia method for use on the Roche Cobas 6000 (c501) automated platform. (United States)

    Seiden-Long, Isolde; Schnabl, Kareena; Skoropadyk, Wendy; Lennon, Nola; McKeage, Arlayne


    Adaptation of the Randox Enzymatic Manual UV Ammonia method to be used on the Roche Cobas 6000 (c501) automated analyzer platform. The Randox ammonia reagent was evaluated for precision, linearity, accuracy and interference from hemolysis, icterus and lipemia on the Roche c501 analyzer. Comparison studies were conducted for the Randox reagent between Roche c501, Siemens Vista, Ortho Vitros 250, and Beckman DxC methods. The Randox reagent demonstrates acceptable within-run (L1=65 μmol/L, CV 3.4% L2=168 μmol/L, CV 1.9%) and between-run precision (L1=29 μmol/L, CV 7.3% L2=102 μmol/L, CV 3.0%), Analytical Measurement Range (7-940 μmol/L), and accuracy. The method interference profile is superior for the Randox method (hemolysis index up to 600, icteric index up to 60, lipemic index up to 100) as compared to the Roche method (hemolysis index up to 200, icteric index up to 10, lipemic index up to 50). Comparison was very good between the Randox reagent and two other wet chemistry platforms. The Randox Enzymatic Manual UV Ammonia reagent is an available alternative to the Roche Cobas c501 reagent. The method is more robust to endogenous interferences and less prone to instrument error flags, thus allowing the majority of clinical specimens to be reported without additional sample handling at our institution. Copyright © 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  15. Serum concentrations of prostate-specific antigen measured using immune extraction, trypsin digestion, and tandem mass spectrometry quantification of LSEPAELTDAVK peptide. (United States)

    Klee, Eric W; Bondar, Olga P; Goodmanson, Marcia K; Trushin, Sergey A; Bergstralh, Eric J; Singh, Ravinder J; Anderson, N Leigh; Klee, George G


    Prostate-specific antigen (PSA) is a 34-kDa glycoprotein with chymotrypsin-like enzyme activity that circulates both in free forms and complexed to various enzyme inhibitors including antichymotrypsin and α2-macroglobulin. Prostate-specific antigen bound to α2-macroglobulin is not detected by commercial PSA immunoassays. To develop a mass spectrometry assay that detects the same forms of PSA as the immunoassays, which could serve as a reference for harmonizing PSA immunoassays. Prostate-specific antigen was immune extracted from serum, trypsin was digested, and the LSEPAELTDAVK peptide was quantitated on an API 5000 spectrometer. Calibrators were made by adding 10% free and 90% antichymotrypsin-bound PSA to female sera. The assay was standardized to the World Health Organization 96/670 reference standard. Validation of clinical utility and comparisons with 2 immunoassays (Roche cobas and Beckman Access) were performed using frozen sera aliquots from 100 men undergoing prostate biopsy (50 negative, 50 with cancer) and 5 serial samples collected over time from 5 men with advanced prostate cancer. The antibody extraction efficiency was greater than 99%. The assay has an analytic range from 1.2 to 76 ng/mL, with precision ranging from 8.6% at 1.5 ng/mL to 5.4% at 27 ng/mL. The mass spectrometry assay correlated well with 2 immunoassays. All 3 assays showed statistically equivalent separation of prostate cancer from benign disease using receiver operating characteristic curve analysis. This mass spectrometry assay can reliably measure PSA concentrations in human serum and could serve as a reference standard for harmonizing PSA immunoassays.

  16. Pediatric Reference Intervals for Transferrin Saturation in the CALIPER Cohort of Healthy Children and Adolescents. (United States)

    Higgins, Victoria; Chan, Man Khun; Adeli, Khosrow


    Transferrin saturation reference intervals specific for age and sex have not been previously reported for the pediatric population. The reference values for transferrin saturation have been previously reported to be lower in children compared to adults, caused by a combination of low serum iron and high serum transferrin levels in children, warranting specific reference intervals. Here we use the original iron and transferrin data from the CALIPER cohort to establish age- and sex-specific pediatric reference intervals for transferrin saturation. Iron and transferrin concentrations were measured in serum samples from the CALIPER cohort of healthy children and adolescents on the Abbott Architect c8000. Transferrin saturation was subsequently calculated and statistically relevant age- and sex-partitions were determined. After removing outliers, age- and sex-specific reference intervals with corresponding 90% confidence intervals were calculated using CLSI C28-A3 guidelines. Transferrin saturation required 3 separate age partitions, with an additional sex partition for 14-<19 year olds. Transferrin saturation was more variable during the first year of life, evident by a wider reference interval, which subsequently narrowed at one year until adolescence. Upon adolescence, a sex difference was apparent with females having lower percent transferrin saturation than males. Age- and sex-specific pediatric reference intervals for transferrin saturation were established based on a large cohort of healthy pediatric subjects. Transference studies suggest that these intervals established using Abbott assays are comparable to those on Beckman, Ortho, Roche, and Siemens assays. Individual laboratories should however verify these reference intervals for their individual instrument and local population as per CLSI guidelines.

  17. Transference of CALIPER pediatric reference intervals to biochemical assays on the Roche cobas 6000 and the Roche Modular P. (United States)

    Higgins, Victoria; Chan, Man Khun; Nieuwesteeg, Michelle; Hoffman, Barry R; Bromberg, Irvin L; Gornall, Doug; Randell, Edward; Adeli, Khosrow


    The Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) has recently established pediatric age- and sex-specific reference intervals for over 85 biochemical markers on the Abbott Architect system. Previously, CALIPER reference intervals for several biochemical markers were successfully transferred from Abbott assays to Roche, Beckman, Ortho, and Siemens assays. This study further broadens the CALIPER database by performing transference and verification for 52 biochemical assays on the Roche cobas 6000 and the Roche Modular P. Using CLSI C28-A3 and EP9-A2 guidelines, transference of the CALIPER reference intervals was attempted for 16 assays on the Roche cobas 6000 and 36 on the Modular P. Calculated reference intervals were further verified using 100 healthy CALIPER samples. Most assays showed strong correlation between assay systems and were transferable from Abbott to the Roche cobas 6000 (81%) and the Modular P (86%). Bicarbonate and magnesium were not transferable on either system and calcium and prealbumin were not transferable to the Modular P. Of the transferable analytes, 62% and 61% were verified on the cobas 6000 and the Modular P, respectively. This study extends the utility of the CALIPER database to two additional analytical systems, which facilitates the broad application of CALIPER reference intervals at pediatric centers utilizing Roche biochemical assays. Transference studies across different analytical platforms can later be collectively analyzed in an attempt to develop common reference intervals across all clinical chemistry instruments to harmonize laboratory test interpretation in diagnosis and monitoring of pediatric disease. Copyright © 2015 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  18. Paraprotein interference with turbidimetric gentamicin assay. (United States)

    Dimeski, Goce; Bassett, Kendra; Brown, Nigel


    Gentamicin due to its low level of resistance and rapid bactericidal activity is commonly used to treat gram-negative bacteria. However, due to its toxic effects it needs to be monitored. To date, no interference has been reported with gentamicin assays. A patient with leg cellulitis and sepsis received a single dose of gentamicin and a sample was sent for gentamicin analysis. The sample showed high blank absorbance readings on Beckman DxC800 and DC800 analysers with various dilutions. A second sample was received and analysed on a Roche Cobas system to obtain a result. A third sample was received 107 hours later with the same results and this sample was then analysed neat and post ethanol precipitation on all the turbidimetric assays available on the DxC800 analyser. The high blank absorbance was observed upon addition of the reactive reagents due to protein precipitation. Although not obvious from the patient protein results, it was shown the presence of high IgM paraprotein, 18.9 g/L (reference range 0.4-2.3 g/L) was the cause of precipitation, giving high blank readings. Of all the other turbidimetric assays, only vancomicin and valproate showed similar high blank absorbance readings. To be able to provide more rapid results it was shown ethanol could be used as a precipitant of proteins in both calibrators and patient samples with acceptable recovery. IgM paraprotein was identified as the cause of interference with the gentamicin, vancomicin and valproate assays. Protein interference in these assays can be overcome by precipitation with ethanol.

  19. Annual symposium on Frontiers in Science

    Energy Technology Data Exchange (ETDEWEB)

    Metzger, N.; Fulton, K.R.


    This final report summarizes activities conducted for the National Academy of Sciences' Annual Symposium on Frontiers of Science with support from the US Department of Energy for the period July 1, 1993 through May 31, 1998. During the report period, five Frontiers of Science symposia were held at the Arnold and Mabel Beckman Center of the National Academies of Sciences and Engineering. For each Symposium, an organizing committee appointed by the NAS President selected and planned the eight sessions for the Symposium and identified general participants for invitation by the NAS President. These Symposia accomplished their goal of bringing together outstanding younger (age 45 or less) scientists to hear presentations in disciplines outside their own and to discuss exciting advances and opportunities in their fields in a format that encourages, and allows adequate time for, informal one-on-one discussions among participants. Of the 458 younger scientists who participated, over a quarter (124) were women. Participant lists for all symposia (1993--1997) are attached. The scientific participants were leaders in basic research from academic, industrial, and federal laboratories in such disciplines as astronomy, astrophysics, atmospheric science, biochemistry, cell biology, chemistry, computer science, earth sciences, engineering, genetics, material sciences, mathematics, microbiology, neuroscience, physics, and physiology. For each symposia, the 24 speakers and discussants on the program were urged to focus their presentations on current cutting-edge research in their field for a scientifically sophisticated but non-specialist audience, and to provide a sense of the experimental data--what is actually measured and seen in the various fields. They were also asked to address questions such as: What are the major research problems and unique tools in their field? What are the current limitations on advances as well as the frontiers? Speakers were asked to provide a

  20. A Retrospective Analysis of Urine Drugs of Abuse Immunoassay True Positive Rates at a National Reference Laboratory. (United States)

    Johnson-Davis, Kamisha L; Sadler, Aaron J; Genzen, Jonathan R


    Urine drug screens are commonly performed to identify drug use or monitor adherence to drug therapy. The purpose of this retrospective study was to evaluate the true positive and false positive rates of one of our in-house urine drug screen panels. The urine drugs of abuse panel studied consists of screening by immunoassay then positive immunoassay results were confirmed by mass spectrometry. Reagents from Syva and Microgenics were used for the immunoassay screen. The screen was performed on a Beckman AU5810 random access automated clinical analyzer. The percent of true positives for each immunoassay was determined. Agreement with previously validated GC-MS or LC-MS-MS confirmatory methods was also evaluated. There were 8,825 de-identified screening results for each of the drugs in the panel, except for alcohol (N = 2,296). The percent of samples that screened positive were: 10.0% for amphetamine/methamphetamine/3,4-methylenedioxy-methamphetamine (MDMA), 12.8% for benzodiazepines, 43.7% for opiates (including oxycodone) and 20.3% for tetrahydrocannabinol (THC). The false positive rate for amphetamine/methamphetamine was ∼14%, ∼34% for opiates (excluding oxycodone), 25% for propoxyphene and 100% for phencyclidine and MDMA immunoassays. Based on the results from this retrospective study, the true positive rate for THC drug use among adults were similar to the rate of illicit drug use in young adults from the 2013 National Survey; however, our positivity rate for cocaine was higher than the National Survey. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email:

  1. First two-reagent vitamin D assay for general clinical chemistry. (United States)

    Saida, Fakhri B; Padilla-Chee, Mario; Dou, Chao; Yuan, Chong


    Vitamin D is a lipid-soluble molecule that plays key physiological roles in the metabolism of calcium, phosphate and magnesium. Recent studies show that deficiency in vitamin D is linked to cardiovascular diseases, autoimmune diseases and cancer. As a result, regular monitoring of 25-OH vitamin D (the main circulating form of vitamin D) is becoming essential. Current 25-OH vitamin D testing methodologies are cumbersome (too many reagents, long incubation times, phase separation) and are not compatible with general clinical chemistry platforms. Here, we report on a novel method to detect 25-OH vitamin D that is fast (results in 10 min or less), simple (two reagents) and compatible with virtually all general clinical chemistry analyzers. An immunoturbidimetric assay for 25-OH vitamin D (the Diazyme EZ Vitamin D Assay) has been developed using nanoparticles and vitamin D-specific antibodies. The performance of the assay kit, which consists of two reagents and five calibrators, was tested on the Beckman AU680 analyzer (AU680). The new assay was precise, sensitive (LOD = 7.2 nmol/L), linear (up to 390.1 nmol/L) and correlated strongly (R 2  > 0.95) with major commercial 25-OH vitamin D assays. Additionally, the assay was found to be the fastest to date, with the first results obtained within 10 min. Throughput on the AU680 was estimated at over 300 tests per hour. The newly developed 25-OH vitamin D assay is fast, precise and accurate. It can be run on most general chemistry analyzers. This assay aims at providing vitamin D-testing capabilities to all clinical chemistry laboratories. Copyright © 2018. Published by Elsevier Inc.

  2. Enhanced radiation response in radioresistant MCF-7 cells by targeting peroxiredoxin II

    Directory of Open Access Journals (Sweden)

    Diaz AJG


    Full Text Available Anthony Joseph Gomez Diaz,1 Daniel Tamae,2 Yun Yen,3 JianJian Li,4 Tieli Wang1 1Department of Chemistry and Biochemistry, California State University at Dominguez Hills, Carson, CA, 2Center of Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania, Philadelphia, PA, 3Department of Clinical and Molecular Pharmacology, Beckman Research Institute of City of Hope National Medical Center, Duarte, CA, 4Department of Radiation Oncology, University of California Davis, Sacramento, CA, USA Abstract: In our previous study, we identified that a protein target, peroxiredoxin II (PrxII, is overexpressed in radioresistant MCF+FIR3 breast-cancer cells and found that its expression and function is associated with breast-cancer radiation sensitivity or resistance. Small interference RNA (siRNA targeting PrxII gene expression was able to sensitize MCF+FIR3 radioresistant breast-cancer cells to ionizing radiation. The major focus of this work was to investigate how the radiation response of MCF+FIR3 radioresistant cells was affected by the siRNA that inhibits PrxII gene expression. Our results, presented here, show that silencing PrxII gene expression increased cellular toxicity by altering cellular thiol status, inhibiting Ca2+ efflux from the cells, and perturbing the intracellular Ca2+ homeostasis. By combining radiotherapy and siRNA technology, we hope to develop new therapeutic strategies that may have potential to enhance the efficacy of chemotherapeutic agents due to this technology's property of targeting to specific cancer-related genes. Keywords: siRNA, PrxII, radiation resistance, Ca2+, MCF+FIR3

  3. Assessing composition and structure of soft biphasic media from Kelvin-Voigt fractional derivative model parameters (United States)

    Zhang, Hongmei; Wang, Yue; Fatemi, Mostafa; Insana, Michael F.


    Kelvin-Voigt fractional derivative (KVFD) model parameters have been used to describe viscoelastic properties of soft tissues. However, translating model parameters into a concise set of intrinsic mechanical properties related to tissue composition and structure remains challenging. This paper begins by exploring these relationships using a biphasic emulsion materials with known composition. Mechanical properties are measured by analyzing data from two indentation techniques—ramp-stress relaxation and load-unload hysteresis tests. Material composition is predictably correlated with viscoelastic model parameters. Model parameters estimated from the tests reveal that elastic modulus E 0 closely approximates the shear modulus for pure gelatin. Fractional-order parameter α and time constant τ vary monotonically with the volume fraction of the material’s fluid component. α characterizes medium fluidity and the rate of energy dissipation, and τ is a viscous time constant. Numerical simulations suggest that the viscous coefficient η is proportional to the energy lost during quasi-static force-displacement cycles, E A . The slope of E A versus η is determined by α and the applied indentation ramp time T r. Experimental measurements from phantom and ex vivo liver data show close agreement with theoretical predictions of the η -{{E}A} relation. The relative error is less than 20% for emulsions 22% for liver. We find that KVFD model parameters form a concise features space for biphasic medium characterization that described time-varying mechanical properties. The experimental work was carried out at the Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Methodological development, including numerical simulation and all data analysis, were carried out at the school of Life Science and Technology, Xi’an JiaoTong University, 710049, China.

  4. Use of an external source (60Co) for 32P detection efficiency determination by the Cerenkov effect, in soil extracts

    International Nuclear Information System (INIS)

    Nascimento Filho, V.F. do.


    The detection of 32 P in aqueous extracts is usually made with the aid of a Geiger-Muller detector, with thin window and sample on a planchet. Presently the technique is being developed of detection of high energy beta particles emitters ( 32 P, 42 K, 86 Rb) through the Cerenkov effect, using a commercial liquid scintillation system. This technique, despite being approximately 30 times more sensitive, has the inconvenience of varying the detection efficiency, mainly for color samples (soil extracts, for instance). From this stems the need for determining the detection efficiency for each sample. The internal standardization and channels ratio methods show a series of drawbacks, mainly the non-reutilization of the samples (1st method) and statistical uncertainty for low activity samples (2nd method). The elimination of these dreawbacks can be achieved through the utilization of the external standardization method. A 60 Co source with 1,4 μCi activity has been adapted to the sample elevator of the detector system, and a comparison was made with the channels ratio method to evaluate the efficiency of 32 P detection in soil extracts (P extraction and fractionation). The external standardization method showed to be more accurate, besides being influenced to a lesser degree by high voltage variation, sample volume and vial types. In the case of large samples, it is advisable to carry out detection in vials filled up to their full capacity; in the case of small samples, the whole volume should be transferred to the vials and completed up to 9 ml for nylon vials,10 ml for glass vials and 11 to 14 ml for polyethilene vials. On the other hand, plastic vials showed higher detection efficiency than ones. As to background radiation, the lowest rates were given by nylon vials and the highest by Beckman glass vials [pt

  5. Cerebrospinal Fluid Total Protein Reference Intervals Derived from 20 Years of Patient Data. (United States)

    McCudden, Christopher R; Brooks, John; Figurado, Priya; Bourque, Pierre R


    Reference intervals are vital for interpretation of laboratory results. Many existing reference intervals for cerebrospinal fluid total protein (CSF-TP) are derived from old literature because of the invasive nature of sampling. The objective of this study was to determine reference intervals for CSF-TP using available patient data. Twenty years of hospital database information was mined for previously reported CSF-TP results. Associated demographic, laboratory, and clinical diagnosis (International Classification of Diseases 9/10 codes) details were extracted. CSF-TP results included 3 different analytical platforms: the Siemens Vista 1500, Beckman Lx20, and Roche Hitachi 917. From an initial data set of 19591 samples, the following exclusion criteria were applied: incomplete data, white blood cells (WBCs) >5 × 10 6 /L, red blood cells (RBCs) >50 × 10 6 /L, and glucose reference intervals were determined using quantile regression. Age- and sex-partitioned intervals were established using the quantile regression equation and splitting age-groups into 5-year bins. CSF-TP showed a marked age dependence, and males had significantly higher CSF-TP than females across all ages. CSF-TP results from the 3 different instruments and manufacturers showed small (approximately 0.04 g/L), but statistically significant, differences. CSF-TP showed weak, but again statistically significant, correlation with WBC and RBC but was independent of serum total protein and creatinine. The age dependence of CSF-TP supports that age-partitioned reference intervals will be more accurate than a single cutoff, particularly in patients with advancing age. © 2017 American Association for Clinical Chemistry.

  6. Commutability of possible external quality assessment materials for cardiac troponin measurement. (United States)

    Zhang, Shunli; Zeng, Jie; Zhang, Chuanbao; Li, Yilong; Zhao, Haijian; Cheng, Fei; Yu, Songlin; Wang, Mo; Chen, Wenxiang


    The measurement of cardiac troponin is crucial in the diagnosis of myocardial infarction. The performance of troponin measurement is most conveniently monitored by external quality assessment (EQA) programs. The commutability of EQA samples is often unknown and the effectiveness of EQA programs is limited. Commutability of possible EQA materials was evaluated. Commercial control materials used in an EQA program, human serum pools prepared from patient samples, purified analyte preparations, swine sera from model animals and a set of patient samples were measured for cTnI with 4 assays including Abbott Architect, Beckman Access, Ortho Vitros and Siemens Centaur. The measurement results were logarithm-transformed, and the transformed data for patient samples were pairwise analyzed with Deming regression and 95% prediction intervals were calculated for each pair of assays. The commutability of the materials was evaluated by comparing the logarithmic results of the materials with the limits of the intervals. Matrix-related biases were estimated for noncommutable materials. The impact of matrix-related bias on EQA was analyzed and a possible correction for the bias was proposed. Human serum pools were commutable for all assays; purified analyte preparations were commutable for 2 of the 6 assay pairs; commercial control materials and swine sera were all noncommutable; swine sera showed no reactivity to Vitros assay. The matrix-related biases for noncommutable materials ranged from -83% to 944%. Matrix-related biases of the EQA materials caused major abnormal between-assay variations in the EQA program and correction of the biases normalized the variations. Commutability of materials has major impact on the effectiveness of EQA programs for cTnI measurement. Human serum pools prepared from patient samples are commutable and other materials are mostly noncommutable. EQA programs should include at least one human serum pool to allow proper interpretation of EQA results.

  7. Commutability of possible external quality assessment materials for cardiac troponin measurement.

    Directory of Open Access Journals (Sweden)

    Shunli Zhang

    Full Text Available The measurement of cardiac troponin is crucial in the diagnosis of myocardial infarction. The performance of troponin measurement is most conveniently monitored by external quality assessment (EQA programs. The commutability of EQA samples is often unknown and the effectiveness of EQA programs is limited.Commutability of possible EQA materials was evaluated. Commercial control materials used in an EQA program, human serum pools prepared from patient samples, purified analyte preparations, swine sera from model animals and a set of patient samples were measured for cTnI with 4 assays including Abbott Architect, Beckman Access, Ortho Vitros and Siemens Centaur. The measurement results were logarithm-transformed, and the transformed data for patient samples were pairwise analyzed with Deming regression and 95% prediction intervals were calculated for each pair of assays. The commutability of the materials was evaluated by comparing the logarithmic results of the materials with the limits of the intervals. Matrix-related biases were estimated for noncommutable materials. The impact of matrix-related bias on EQA was analyzed and a possible correction for the bias was proposed.Human serum pools were commutable for all assays; purified analyte preparations were commutable for 2 of the 6 assay pairs; commercial control materials and swine sera were all noncommutable; swine sera showed no reactivity to Vitros assay. The matrix-related biases for noncommutable materials ranged from -83% to 944%. Matrix-related biases of the EQA materials caused major abnormal between-assay variations in the EQA program and correction of the biases normalized the variations.Commutability of materials has major impact on the effectiveness of EQA programs for cTnI measurement. Human serum pools prepared from patient samples are commutable and other materials are mostly noncommutable. EQA programs should include at least one human serum pool to allow proper interpretation of

  8. Measurement of isotopic uranium in water for compliance monitoring by liquid scintillation counting with alpha/beta discrimination

    International Nuclear Information System (INIS)

    Venso, E.A.S.


    A simple and inexpensive method is described for analysis of uranium (U) activity and mass in water by liquid scintillation counting using α/β discrimination. This method appears to offer a solution to the need for an inexpensive protocol for monitoring U activity and mass simultaneously and an alternative to the potential inaccuracy involved when depending on the mass-to-activity conversion factor or activity screen. U is extracted virtually quantitatively into 20 ml extractive scintillator from a 1-ell aliquot of water acidified to less than pH 2. After phase separation, the sample is counted for a 20-minute screening count with a minimum detection level of 0.27 pCi ell -1 . α-particle emissions from the extracted U are counted with close to 100% efficiency with a Beckman LS6000 LL liquid scintillation counter equipped with pulse-shape discrimination electronics. Samples with activities higher than 10 pCi ell -1 are recounted for 500-1000 minutes for isotopic analysis. Isotopic analysis uses events that are automatically stored in spectral files and transferred to a computer during assay. The data can be transferred to a commercially available spreadsheet and retrieved for examination or data manipulation. Values for three readily observable spectral features can be rapidly identified by data examination and substituted into a simple formula to obtain 234 U/ 238 U ratio for most samples. U mass is calculated by substituting the isotopic ratio value into a simple equation. The utility of this method for the proposed compliance monitoring of U in public drinking water supplies was field tested with a survey of drinking water from Texas supplies that had previously been known to contain elevated levels of gross α activity. U concentrations in 32 samples from 27 drinking water supplies ranged from 0.26 to 65.5 pCi ell -1 , with seven samples exceeding the proposed Maximum Contaminant Level

  9. Evaluation of the serum zinc level in erosive and non-erosive oral lichen planus. (United States)

    Gholizadeh, N; Mehdipour, M; Najafi, Sh; Bahramian, A; Garjani, Sh; Khoeini Poorfar, H


    Lichen planus is a chronic inflammatory immunologic-based disease involving skin and mucosa. This disease is generally divided into two categories: erosive and non-erosive. Many etiologic factors are deliberated regarding the disease; however, the disorders of immune system and the role of cytotoxic T-lymphocytes and monocytes are more highlighted. Zinc is an imperative element for the growth of epithelium and its deficiency induces the cytotoxic activity of T-helper2 cells, which seems to be associated with lichen planus. This study was aimed to evaluate the levels of serum zinc in erosive and non-erosive oral lichen planus (OLP) and to compare it with the healthy control group to find out any feasible inference. A total of 22 patients with erosive oral lichen planus, 22 patients with non erosive OLP and 44 healthy individuals as the control group were recruited in this descriptive-comparative study. All the participants were selected from the referees to the department of oral medicine, school of dentistry, Tabriz University of Medical Sciences. Serum zinc level was examined for all the individuals with liquid-stat kit (Beckman Instruments Inc.; Carlsbad, CA). Data were analyzed by adopting the ANOVA and Tukey tests, using SPSS 16 statistical software. The mean age of patients with erosive and non-erosive LP was 41.7 and 41.3 years, respectively. The mean age of the healthy control group was 34.4 years .The mean serum zinc levels in the erosive and non erosive lichen planus groups and control groups were 8.3 (1.15), 11.15 (0.92) and 15.74 (1.75) μg/dl respectively. The difference was statistically significant (plichen planus. This finding may probably indicate the promising role of zinc in development of oral lichen planus.

  10. Screening for fetal chromosome abnormalities during the second trimester

    International Nuclear Information System (INIS)

    Dong Hui; Li Ming; Li Ping


    Objective: To develop a pre -natal screening program for fetal chromosome abnormalities based on risk values calculated from maternal serum markers levels during the second trimester. Methods: Serum levels of AFP, β-HCG, uE 3 were determined with CLIA in 1048 pregnant women during 14-21w gestation period and the results were analyzed with a specific software (screening program for Down' s syndrome developed by Beckman) for the risk rate. In those women defined as being of high risk rate, cells from amniotic fluid or umbilical cord blood were studied for karyotype analysis. Results: Of these 1048 women, 77 were designated as being of high risk rate for several chromosome abnormalities i.e. Down's syndrome, open spina bifida and trisomy -18 syndrome (overall positive rate 7.3%). Further fetal chromosome study in 31 of them revealed three proven cases of abnormality. Another cord blood study was performed in a calculated low risk rate case but with abnormal sonographic finding at 31 w gestation and proved to be abnormal (software study false negative). The remaining 46 high risk rate cases either refused future study (n=35) or were lost for follow-up (n=11). Fortunately, all the 35 women refused further study gave birth to normal babies without any chromosome abnormalities discovered on peripheral blood study. Besides, in a trial study, five high risk rate women were again evaluated a few weeks later but with tremendous difference between the results. Conclusion: The present program proves to be clinically useful but needs further study and revision. Many factors may influence the result of the analysis and the duration of gestation period in weeks should be as accurate as possible. At present, in order to avoid getting false negatives, we don't advise a second check in 'high risk' cases. (authors)

  11. A fast radiochemical procedure to measure Tc-99 in environmental samples and gaseous and liquid effluents

    International Nuclear Information System (INIS)

    Luxenburger, H.J.; Schuettelkopf, H.; Bohn, B.


    Liquid effluent samples are collected in polyethylene bottles. Sampling in the gaseous effluents relies on conventional NaOH scrubbers. No noticeable adsorption on the vessel walls takes place in HNO 3 /H 2 O 2 and NaOH, respectively, in the plastic botteles used. A known amount of Tc-95m is added in order to determine the chemical yield prior to the analysis. The pertechnetate in the samples is first fixed on an anionexchanger. Following elution with perchlorate/sulfite solution TC is coprecipitated with iron(II) hydroxide. Having dissolved the precipitation in H 2 O 2 /H 2 SO 4 one extracts with TBP/alkane. To achieve a better decontamination from other nuclides a re-extraction is performed with NaOH and after acidification with H 2 SO 4 a further extraction using TBP/Alkane. Before the measurement of the sample the alkane is eliminated in a rotation evaporator and the remaining TBP is dissolved directly in the liquid scintillator. Other beta emitters generated as fission products in the course of nuclear fission are satisfactorily separated during the analysis. The decontamination factors for I, Se, Te, Sb, and Ru are > 10 5 . The measurements are carried out with a Beckman LS 7800 instrument. The optimum channel setting is found from the spectra of the two nuclides Tc-95m and Tc-99. The quenching plots are determined accordingly. The measurements are evaluated with a table computer. Chemical yields of 50-90% are achieved. The detection limit for 600 minutes of measuring time is 1 pCi per sample. One person is capable of performing about 15 analyses per week using the method described. (orig./HP) [de

  12. SNP genotypes of olfactory receptor genes associated with olfactory ability in German Shepherd dogs. (United States)

    Yang, M; Geng, G-J; Zhang, W; Cui, L; Zhang, H-X; Zheng, J-L


    To find out the relationship between SNP genotypes of canine olfactory receptor genes and olfactory ability, 28 males and 20 females from German Shepherd dogs in police service were scored by odor detection tests and analyzed using the Beckman GenomeLab SNPstream. The representative 22 SNP loci from the exonic regions of 12 olfactory receptor genes were investigated, and three kinds of odor (human, ice drug and trinitrotoluene) were detected. The results showed that the SNP genotypes at the OR10H1-like:c.632C>T, OR10H1-like:c.770A>T, OR2K2-like:c.518G>A, OR4C11-like:c.511T>G and OR4C11-like:c.692G>A loci had a statistically significant effect on the scenting abilities (P odor influenced the performances of the dogs (P odor at the following loci: OR10H1-like:c.632C>T, OR10H1-like:c.770A>T, OR4C11-like:c.511T>G and OR4C11-like:c.692G>A (P dogs with genotype CC at the OR10H1-like:c.632C>T, genotype AA at the OR10H1-like:c.770A>T, genotype TT at the OR4C11-like:c.511T>G and genotype GG at the OR4C11-like:c.692G>A loci did better at detecting the ice drug. We concluded that there was linkage between certain SNP genotypes and the olfactory ability of dogs and that SNP genotypes might be useful in determining dogs' scenting potential. © 2015 Stichting International Foundation for Animal Genetics.

  13. The Putative Role of the Non-Gastric H+/K+-ATPase ATP12A (ATP1AL1 as Anti-Apoptotic Ion Transporter: Effect of the H+/K+ ATPase Inhibitor SCH28080 on Butyrate-Stimulated Myelomonocytic HL-60 Cells

    Directory of Open Access Journals (Sweden)

    Martin Jakab


    Full Text Available Background/Aims: The ATP12A gene codes for a non-gastric H+/K+ ATPase, which is expressed in a wide variety of tissues. The aim of this study was to test for the molecular and functional expression of the non-gastric H+/K+ ATPase ATP12A/ATP1AL1 in unstimulated and butyrate-stimulated (1 and 10 mM human myelomonocytic HL-60 cells, to unravel its potential role as putative apoptosis-counteracting ion transporter as well as to test for the effect of the H+/K+ ATPase inhibitor SCH28080 in apoptosis. Methods: Real-time reverse-transcription PCR (qRT-PCR was used for amplification and cloning of ATP12A transcripts and to assess transcriptional regulation. BCECF microfluorimetry was used to assess changes of intracellular pH (pHi after acute intracellular acid load (NH4Cl prepulsing. Mean cell volumes (MCV and MCV-recovery after osmotic cell shrinkage (Regulatory Volume Increase, RVI were assessed by Coulter counting. Flow-cytometry was used to measure MCV (Coulter principle, to assess apoptosis (phosphatidylserine exposure to the outer leaflet of the cell membrane, caspase activity, 7AAD staining and differentiation (CD86 expression. Results: We found by RT-PCR, intracellular pH measurements, MCV measurements and flow cytometry that ATP12A is expressed in human myelomonocytic HL-60 cells. Treatment of HL-60 cells with 1 mM butyrate leads to monocyte-directed differentiation whereas higher concentrations (10 mM induce apoptosis as assessed by flow-cytometric determination of CD86 expression, caspase activity, phosphatidylserine exposure on the outer leaflet of the cell membrane and MCV measurements. Transcriptional up-regulation of ATP12A and CD86 is evident in 1 mM butyrate-treated HL-60 cells. The H+/K+ ATPase inhibitor SCH28080 (100 µM diminishes K+-dependent pHi recovery after intracellular acid load and blocks RVI after osmotic cell shrinkage. After seeding, HL-60 cells increase their MCV within the first 24 h in culture, and subsequently

  14. Macronutrients leaf contents of corn in intercropping with forages of genus Panicum and Urochloa in simultaneous seeding

    Directory of Open Access Journals (Sweden)

    Denise Tsuzukibashi


    Full Text Available The planting of crops in degraded pasture areas is a formula used for decades by farmers to recover the productive capacity of pastures and soils. The integrated crop-livestock (ICL consists of different production systems of grains, fibers, wood, meat, milk and agro-deployed in the same area, in intercrop, rotation or succession. Typically this integration mainly involves the planting of grain and pasture in the recovery or deployment. This work aimed to evaluate the macronutrients leaf contents of irrigated corn intercropped with forages of the genus Panicum and Urochloa simultaneously to sown corn. The experiment was conducted at the Farm for Teaching, Research and Extension, Faculty of Engineering - UNESP, Ilha Solteira in an Oxisol in Savannah conditions, in experimental area that had a history of no-tillage to 8 years (previous crop corn. The experimental design used was randomized blocks with four replications and five treatments: Panicum maximum cv. Tanzania sown simultaneously (CTS corn; Panicum maximum cv. Mombaça sown simultaneously (CMS to corn; Urochloa brizantha cv. Xaraes sown simultaneously (CBS corn; Urochloa ruziziensis sown simultaneously (CRS to corn, and corn without intercropping (CWI. The seeds of grasses were sown in spacing of 0.34 m, being sown with a seed drill with disc coulters mounted mechanism for no-tillage system at a depth of 0.03 m. There was no significant difference between the single corn tillage and intercropping with different modalities of forage genus Panicum and Urochloa to the leaf contents of N, P, K, Ca and Mg, demonstrating the non-compete forages with corn in a intercrop on the absorption of these nutrients. In respect to S, CTS presented higher content of S foliar when compared to CWI (Table 1. The absorption of nutrients by corn are not affect by the intercrop with forages of the genus Panicum and Urochloa, in simultaneously sown.

  15. PSA Isoforms' Velocities for Early Diagnosis of Prostate Cancer. (United States)

    Heidegger, Isabel; Klocker, Helmut; Pichler, Renate; Horninger, Wolfgang; Bektic, Jasmin


    Free prostate-specific antigen (fPSA) and its molecular isoforms are suggested for enhancement of PSA testing in prostate cancer (PCa). In the present study we evaluated whether PSA isoforms' velocities might serve as a tool to improve early PCa diagnosis. Our study population included 381 men who had undergone at least one ultrasound-guided prostate biopsy whose pathologic examination yielded PCa or showed no evidence of prostatic malignancy. Serial PSA, fPSA, and proPSA measurements were performed on serum samples covering 7 years prior to biopsy using Beckmann Coulter Access immunoassays. Afterwards, velocities of PSA (PSAV), fPSA% (fPSA%V), proPSA% (proPSA%V) and the ratio proPSA/PSA/V were calculated and their ability to discriminate cancer from benign disease was evaluated. Among 381 men included in the study, 202 (53%) were diagnosed with PCa and underwent radical prostatectomy at our Department. PSAV, fPSA%V, proPSA%V as well as proPSA/PSA/V were able to differentiate significantly between PCa and non-cancerous prostate. The highest discriminatory power between cancer and benign disease has been observed two and one year prior to diagnosis with all measured parameters. Among all measured parameters, fPSA%V showed the best cancer specificity of 45.3% with 90% of sensitivity. In summary, our results highlight the value of PSA isoforms' velocity for early detection of PCa. Especially fPSA%V should be used in the clinical setting to increase cancer detection specificity. Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  16. Prevalence of anemia and associated factors in child bearing age women in riyadh, saudi arabia. (United States)

    Alquaiz, Aljohara M; Gad Mohamed, Ashry; Khoja, Tawfik A M; Alsharif, Abdullah; Shaikh, Shaffi Ahamed; Al Mane, Hamad; Aldiris, Abdallah; Kazi, Ambreen; Hammad, Durdana


    Objective. To determine the prevalence and risk factors for anemia in child bearing age women in Riyadh, Saudi Arabia. Design. Cross-sectional survey was conducted using two-stage cluster sampling. 25 clusters (primary health care centers (PHCC)) were identified from all over Riyadh, and 45-50 households were randomly selected from each cluster. Eligible women were invited to PHCC for questionnaire filling, anthropometric measurements, and complete blood count. Blood hemoglobin was measured with Coulter Cellular Analysis System using light scatter method. Setting. PHCC. Subjects. 969 (68%) women out of 1429 women were included in the analysis. Results. Mean hemoglobin was 12.35 (±1.80) g/dL, 95% CI 12.24-12.46 with interquartile range of 1.9. Anemia (Hb women. Mean (±SD) for MCH, MCV, MCHC, and RDW was 79.21 (±12.17) fL, 26.37 (±6.21) pg, 32.36 (±4.91) g/dL, and 14.84 (±4.65)%, respectively. Multivariate logistic regression revealed that having family history of iron deficiency anemia (OR 2.91, 95% CI 1.78-4.76) and infrequent intake of meat (OR 1.54, 95%CI 1.15-2.05) were associated with increased risk of anemia, whereas increasing body mass index (OR 0.95, 95% CI 0.92-0.97) was associated with reduced risk of anemia. Conclusion. Women should be educated about proper diet and reproductive issues in order to reduce the prevalence of anemia in Saudi Arabia.

  17. The erythrocyte sedimentation rates: some model experiments. (United States)

    Cerny, L C; Cerny, E L; Granley, C R; Compolo, F; Vogels, M


    In order to obtain a better understanding of the erythrocyte sedimentation rate (ESR), several models are presented. The first directs attention to the importance of geometrical models to represent the structure of mixtures. Here it is our intention to understand the effect of the structure on the packing of red blood cells. In this part of the study, "Cheerios" (trademark General Mills) are used as a macroscopic model. It is interesting that a random sampling of "Cheerios" has the same volume distribution curve that is found for erythrocytes with a Coulter Sizing Apparatus. In order to examine the effect of rouleaux formation, the "Cheerios" are stacked one on top of another and then glued. Rouleaux of 2,3,4,5, 7 and 10 discs were used. In order to examine a more realistic biological model, the experiments of Dintenfass were used. These investigations were performed in a split-capillary photo viscometer using whole blood from patients with a variety of diseases. The novel part of this research is the fact that the work was performed at 1g and at near zero gravity in the space shuttle "Discovery." The size of the aggregates and/or rouleaux clearly showed a dependence upon the gravity of the experiment. The purpose of this model was to examine the condition of self-similarity and fractal behavior. Calculations are reported which clearly indicate that there is general agreement in the magnitude of the fractal dimension from the "Cheerios" model, the "Discovery" experiment with those determined with the automatic sedimentimeter. The final aspect of this work examines the surface texture of the sedimention tube. A series of tubes were designed with "roughened" interiors. A comparison of the sedimentation rates clearly indicates a more rapid settling in "roughened" tubes than in ones with a smooth interior surface.

  18. Prevalence of Beta-Thalassemia in premarital screening in Al-Hassa, Saudi Arabia

    International Nuclear Information System (INIS)

    Al-Suliman, A.


    The Al-Hassa area is one of the regions in Saudi Arabia where hemoglobinopathies are prevalent. The Saudi Ministry of Health designed a protocol for premarital testing after the royal decree in December 2003. The protocol was implemented in a February 2004 order. The aim of this study was to determine the prevalence of beta-thalassemia trait among subjects coming for premarital screening in the Al-Hassa area. From February 2004 to November 2004, healthy subjects coming to six marriages consultation centers in the Al-Hassa area underwent routine mandatory tests. Subjects were considered to have beta-thalassemia trait if they had a mean corpuscular volume (MCV), 80 fL and/or a mean corpuscular hemoglobin (MCH) 3.2%. Venous blood was taken into ETDA tube and the complete blood count and red blood cell indices were measured by a Coulter automated cell counter on the same day of hemoglobin collection. Electrophoresis was done on cellulose acetate. All Saudi participants (n=8918), including 4218 (47.3%) males and 4700 (52.7%) females were screened. The prevalence of beta-thalassemia trait with high hemoglobin A2 and microcytic hypochromic anemia was 3.4% (307/8918). In countries with a high prelevance of hemoglobinopathies, a premarital screening program is helpful for identification and prevention of high-risk marriages. With a 3.4% prevalence of beta-thalassemia trait in premarital couples, future comprehensive programs are needed to know the actual prevalence of beta-thalassemia in Al-Hassa. (author)

  19. Climate contributes to zonal forest mortality in Southern California's San Jacinto Mountains (United States)

    Fellows, A.; Goulden, M.


    An estimated 4.6 million trees died over ~375,000 acres of Southern California forest in 2002-2004. This mortality punctuated a decline in forest health that has been attributed to air pollution, stem densification, or drought. Bark beetles were the proximate cause of most tree death but the underlying cause of this extensive mortality is arguably poor forest health. We investigated the contributions that climate, particularly drought, played in tree mortality and how physiological drought stress may have structured the observed patterns of mortality. Field surveys showed that conifer mortality was zonal in the San Jacinto Mountains of Southern California. The proportion of conifer mortality increased with decreasing elevation (p=0.01). Mid-elevation conifers (White Fir, Incense Cedar, Coulter Pine, Sugar Pine, Ponderosa and Jeffrey Pine) died in the lower portions of their respective ranges, which resulted in an upslope lean in species’ distribution and an upslope shift in species’ mean elevation. Long-term precipitation (P) is consistent with elevation over the conifer elevation range (p=0.43). Potential evapotranspiration (ET) estimated by Penman Monteith declines with elevation by nearly half over the same range. These trends suggest that ET, more than P, is critical in structuring the elevational trend in drought stress and may have contributed to the patterns of mortality that occurred in 2002-04. Physiological measurements in a mild drought year (2009) showed late summer declines in plant water availability with decreasing elevation (p < 0.01) and concomitant reductions in carbon assimilation and stomatal conductance with decreasing elevation. We tie these observations together with a simple water balance model.

  20. p53 mutation, allele loss on chromosome 17p, and DNA content in ovarian carcinoma. (United States)

    McManus, D T; Murphy, M; Arthur, K; Hamilton, P W; Russell, S E; Toner, P G


    The aim of this investigation was to explore the relationships between p53 mutation, DNA aneuploidy, 17p deletions, and clinical stage in ovarian cancer. Nuclear suspensions were obtained by tissue disaggregation, stained with propidium iodide, and analysed on a Coulter EPICS Elite flow cytometer. DNA cell cycle analysis was performed using Multicycle software (Phoenix Flow Systems). DNA extracted from paraffin-embedded archival carcinomas/non-tumour tissue was used as template for PCR amplification of the microsatellite dinucleotide repeat polymorphism D17S513, a locus telomeric to p53 on 17p13.1. Allele loss at D17S513 was detected in 64.5 per cent of carcinomas (20 of 31 informative cases). DNA aneuploidy was detected in 20 of 54 (37 per cent) carcinomas. Eight of ten cases previously shown to harbour p53 mutations showed aneuploid DNA content. Although ten other DNA aneuploid cases had shown no p53 mutations, the results show a statistically significant association between p53 mutation and DNA aneuploidy (P p53 mutant cases compared with those showing no p53 mutation (P = 0.02). There was also a significant association between p53 mutations and stage, between ploidy and stage, and between allelic deletions at D17S513 or p53 and stage, but not between these allelic deletions and ploidy. p53 mutations appear to be associated with DNA aneuploidy in ovarian cancer independently of 17p deletions. p53 mutations, DNA aneuploidy, and 17p deletions are associated with late stage.

  1. Formation of microscopic particles during the degradation of different polymers. (United States)

    Lambert, Scott; Wagner, Martin


    This study investigated the formation and size distribution of microscopic plastic particles during the degradation of different plastic materials. Particle number concentrations in the size range 30 nm-60 μm were measured by nanoparticle tracking analysis (NTA) and Coulter Counter techniques. Each of the plastics used exhibited a measureable increase in the release of particles into the surrounding solution, with polystyrene (PS) and polylactic acid (PLA) generating the highest particle concentrations. After 112 d, particle concentrations ranged from 2147 particles ml(-1) in the control (C) to 92,465 particles ml(-1) for PS in the 2-60 μm size class; 1.2 × 10(5) particles ml(-1) (C) to 11.6 × 10(6) for PLA in the 0.6-18 μm size class; and 0.2 × 10(8) particles ml(-1) (C) to 6.4 × 10(8) particles ml(-1) for PS in the 30-2000 nm size class (84 d). A classification of samples based on principal component analysis showed a separation between the different plastic types, with PLA clustering individually in each of the three size classes. In addition, particle size distribution models were used to examine more closely the size distribution data generated by NTA. Overall, the results indicate that at the beginning of plastic weathering processes chain scission at the polymer surface causes many very small particles to be released into the surrounding solution and those concentrations may vary between plastic types. Copyright © 2016 Elsevier Ltd. All rights reserved.


    Directory of Open Access Journals (Sweden)

    Ricardo Castillo


    Full Text Available This article is an advance of a wider investigation, in which the objective is to analyze overall maintenance management in public institutions of higher education in the municipality Cabimas, hence in this development describes the elements of the management maintenance present in these institutions. Authors were consulted such as Duffuaa et al. (2010, Clemenza (2010, Zambrano and Leal (2006 , Robbins and Coulter (2005 y Martínez (2007 among others. The research is descriptive, non-experimental   design, transactional  and  field.  We  used  a population   census,  for  a  total  of   twenty-three  (23   research  subjects,  belonging  to  the Universidad   del   Zulia    Núcleo   Costa   Oriental   del   Lago,  Universidad    Nacional Experimental  Rafael  Maria Baralt and  Instituto Universitario Tecnológico  Cabimas. To collect data, a questionnaire was composed of twenty-two (22 items, which was validated by the trial of six (6 experts, with 0.99 reliability by Cronbach alpha coefficient.  We conclude that the elements planning, organizing, scheduling, execution and control, have a high presence in maintenance management, not the human resource and security elements.

  3. Relationship between Neutrophil-to-Lymphocyte Ratio and Inflammatory Markers in Sickle Cell Anaemia Patients with Proteinuria

    Directory of Open Access Journals (Sweden)

    Mathias Abiodun Emokpae


    Full Text Available The renal functions and structure in sickle cell anaemia (SCA patients may be affected by chronic haemodynamic changes and consequences of vaso-occlusive events in the renal medulla. Few reports on neutrophil-to-lymphocyte (NLR and platelet-to-lymphocyte (PLR ratios in SCA patients in Africans exist in the literature. This study correlates the values of NLR and PLR with measured traditional inflammatory markers in SCA patients with and without proteinuria and impaired kidney function (defined in this study as estimated glomerular filtration rate (eGFR, less than 60 mL/min/1.73 m2. Full blood count, C-reactive protein (CRP, and fibrinogen were assayed in 150 SCA patients and 50 control subjects using Coulter Haematology analyser (CELL DYE 37000 and ELISA method, respectively. The NLR and PLR were calculated by dividing absolute neutrophil or platelet counts by absolute lymphocyte count. Fibrinogen, CRP, NLR, and PLR increased progressively (p < 0.001 in SCA patients with or without proteinuria, with the highest values seen in those with impaired renal function. NLR correlated positively with CRP and fibrinogen in SCA patients without proteinuria (p < 0.001, with proteinuria (p < 0.001, and impaired renal function (p < 0.05. A positive relationship was also observed between NLR and fibrinogen in the control subjects. The need to determine cut-off values for these leukocyte ratios to be used in identifying those patients at risk and in the general management of SCA patients is suggested.

  4. Quantificação de células CD 34+ em sangue periférico, produto de aférese e cordão umbilical: estudo comparativo de três diferentes metodologias

    Directory of Open Access Journals (Sweden)

    Bacal Nydia S.


    Full Text Available A quantificação das células CD34+ em sangue periférico é utilizada para determinar o melhor momento de iniciar a aférese, enquanto que na leucoaférese e no sangue de cordão umbilical determinam a quantidade de células CD34+ para o transplante de células progenitoras. O objetivo deste trabalho foi comparar três diferentes metodologias de quantificação de células CD34+. Foram utilizados três diferentes tipos de amostras: a 32 amostras de sangue periférico, coletadas de pacientes estimulados com G-CSF 50 mg/Kg/dose total, sem quimioterapia na mobilização. b 31 amostras de produto de aférese de pacientes estimulados com o mesmo protocolo de G-CSF. c 20 amostras de sangue de cordão coletadas em CPDA-1, por punção de veia umbilical. As amostras permaneceram à temperatura ambiente no máximo até 24h da análise. O citômetro de fluxo utilizado foi o Epics XL-MCL (Coulter com os protocolos de dupla plataforma: ISHAGE e Mulhouse modificado para análise de maior numero de eventos, e o citômetro Imagn 2000 (Biometric Imaging de plataforma única, conforme técnicas recomendadas. Os anticorpos monoclonais utilizados foram: CD45-FITC, CD34-PE, e isotipo IgG1-PE da Immunotech. As análises estatísticas foram: ANOVA e correlação de teste t de Student. Os resultados não apresentaram diferenças estatisticamente significativas nos três métodos.

  5. Spatial variation in the community of insects associated with the flowers of Pachycereus weberi (Caryophyllales: Cactaceae). (United States)

    Figueroa-Castro, Dulce María; Valverde, Pedro Luis; Vite, Fernando; Carrillo-Ruiz, Hortensia


    The positive relationship between productivity and species diversity is well-known. Insect communities associated with the flowers of Cactaceae species represent an interesting system to explore the productivity-diversity relationship because branches facing the equator receive more photosynthetically active radiation and have higher productivity. Thus, flowers with contrasting orientations within an individual, and even within a single branch, might differ in productivity. Therefore, higher abundance, species richness, and diversity are expected for the insect communities associated with south-facing flowers. This hypothesis was tested in Pachycereus weberi (J.M. Coulter) Backeberg (Cactaceae). Insects within flowers with contrasting orientations were collected and its abundance, richness, and diversity were estimated. We also asked if insects prefer big flowers. Thus, flower volume was estimated and regression analyses were conducted to test if there is a positive relationship between flower size and insect abundance. Flower orientation did not affect species richness. However, species abundance and diversity were different in flowers with contrasting orientations. In general, species abundance was higher in flowers facing southwards than in north-facing flowers. On the contrary, species diversity was higher in north-facing flowers. Abundance of Coleoptera was explained by flower volume in south-facing flowers. Contrary to our hypothesis, total diversity was greater in the less productive oriented flowers. Three possible explanations are discussed to explain the low diversity found in the highly productive, south-facing flowers. Our study provides evidence for the effects of productivity on the structure of insect communities at a very small-scale.

  6. Etiology of anemia of blood donor candidates deferred by hematologic screening

    Directory of Open Access Journals (Sweden)

    Michel Alves da Silva


    Full Text Available OBJECTIVE: Iron deficiency is the most common cause of anemia and one of the main factors in the clinical deferral of blood donors. This fact prompted the current study that aimed to determine the prevalence and etiology of anemia in blood donor candidates and to evaluate the hematological screening technique used for the exclusion of these donors. METHODS: This was a prospective study that compared two groups (Anemic and Non-anemic. Initially screening for anemia was performed by manually measuring hemoglobin (Bioclin® Kit; the results were subsequently compared with an automated screening method (Coulter T-890. The etiology was investigated by hemoglobin electrophoresis in alkaline and acid pH, Hb A2 dosage and measurement of the ferritin concentration by immunoagglutination. Differences and associations of interest were analyzed using the Yates and McNemar's Chi-square tests and the Fisher, Mann-Whitney, Wilcoxon and Kruskal-Wallis tests. RESULTS: The deferral rate due to anemia was 4.2%; iron deficiency was identified in 37.5% and beta thalassemia in 9.3% of the excluded candidates. There was a significant discrepancy between the two techniques used to measure hemoglobin with 38.1% of initially deferred donors presenting normal hemoglobin levels by the automated method. CONCLUSION: The results show a high rate of blood donors being deferred for anemia and confirm that iron deficiency is the most prevalent cause. The discrepancies found by comparing screening methods suggest that hemoglobin and hematocrit levels should be confirmed before deferring a donor due to anemia; this may increase supplies in blood banks.

  7. Determination of perfluorobutane in rat blood by automatic headspace capillary gas chromatography and selected ion monitoring mass spectrometry. (United States)

    Hvattum, E; Normann, P T; Oulie, I; Uran, S; Ringstad, O; Skotland, T


    A new contrast agent (Sonazoid; NC100100) for ultrasound imaging has been developed. It is an aqueous suspension of lipid stabilised perfluorobutane (PFB) gas microbubbles. An automatic headspace capillary gas-chromatographic mass spectrometric method using electron impact ionisation was developed for analysis of Sonazoid PFB in rat blood. The calibration standards were gaseous PFB dissolved in ethanol in the range of 0.5-5000 ng PFB. Fluorotrichloromethane (CFC 11) was used as an internal standard of the method and the MS detector was set to single ion monitoring of the base fragment ions of PFB (m/z 69 and 119) and CFC 11 (m/z 101). The calibration graph, made by plotting the peak area ratios of PFB (m/z 69) to CFC 11(m/z 101) against the amount of PFB, was fitted to a second-order polynomial equation with weighting 1/y2 and found to be reproducible. The limit of quantification of the method was set to 0.4 ng PFB. The between-day variation of the method was below 9.2% relative standard deviation (RSD) and the within-day variation of the method was below 7.6% RSD. The accuracy of the method, as compared to Coulter counter, was estimated by determination of PFB in samples where Sonazoid was added to saline and found to range from 91.5% to 105.2%. PFB, added as Sonazoid, was found to be stable for at least 7 months in rat blood samples when stored at -20 degrees C.

  8. Membrane permeability of the human granulocyte to water, dimethyl sulfoxide, glycerol, propylene glycol and ethylene glycol. (United States)

    Vian, Alex M; Higgins, Adam Z


    Granulocytes are currently transfused as soon as possible after collection because they rapidly deteriorate after being removed from the body. This short shelf life complicates the logistics of granulocyte collection, banking, and safety testing. Cryopreservation has the potential to significantly increase shelf life; however, cryopreservation of granulocytes has proven to be difficult. In this study, we investigate the membrane permeability properties of human granulocytes, with the ultimate goal of using membrane transport modeling to facilitate development of improved cryopreservation methods. We first measured the equilibrium volume of human granulocytes in a range of hypo- and hypertonic solutions and fit the resulting data using a Boyle-van't Hoff model. This yielded an isotonic cell volume of 378 μm(3) and an osmotically inactive volume of 165 μm(3). To determine the permeability of the granulocyte membrane to water and cryoprotectant (CPA), cells were injected into well-mixed CPA solution while collecting volume measurements using a Coulter Counter. These experiments were performed at temperatures ranging from 4 to 37°C for exposure to dimethyl sulfoxide, glycerol, ethylene glycol, and propylene glycol. The best-fit water permeability was similar in the presence of all of the CPAs, with an average value at 21°C of 0.18 μmatm(-1)min(-1). The activation energy for water transport ranged from 41 to 61 kJ/mol. The CPA permeability at 21°C was 6.4, 1.0, 8.4, and 4.0 μm/min for dimethyl sulfoxide, glycerol, ethylene glycol, and propylene glycol, respectively, and the activation energy for CPA transport ranged between 59 and 68 kJ/mol. Copyright © 2013 Elsevier Inc. All rights reserved.

  9. Cell diameter measurements obtained with a handheld cell counter could be used as a surrogate marker of G2/M arrest and apoptosis in colon cancer cell lines exposed to SN-38

    International Nuclear Information System (INIS)

    Tahara, Makiko; Inoue, Takeshi; Miyakura, Yasuyuki; Horie, Hisanaga; Yasuda, Yoshikazu; Fujii, Hirofumi; Kotake, Kenjiro; Sugano, Kokichi


    Highlights: •Chemo-sensitivity to SN-38 was assayed by the automated cell counter. •Colon cancer cell line, HCT116 cells were more sensitive to SN-38 than HT29 cells. •Increase of cell size reflects G2/M arrest. •Appearance of small particles indicates cell apoptosis. -- Abstract: In vitro assessment of chemosensitivity are important for experiments evaluating cancer therapies. The Scepter 2.0 cell counter, an automated handheld device based on the Coulter principle of impedance-based particle detection, enables the accurate discrimination of cell populations according to cell size and volume. In this study, the effects of SN-38, the active metabolite of irinotecan, on the colon cancer cell lines HCT116 and HT29 were evaluated using this device. The cell count data obtained with the Scepter counter were compared with those obtained with the 3 H-thymidine uptake assay, which has been used to measure cell proliferation in many previous studies. In addition, we examined whether the changes in the size distributions of these cells reflected alterations in the frequency of cell cycle arrest and/or apoptosis induced by SN-38 treatment. In our experiments using the Scepter 2.0 cell counter, the cell counts were demonstrated to be accurate and reproducible measure and alterations of cell diameter reflected G2/M cell cycle arrest and apoptosis. Our data show that easy-to-use cell counting tools can be utilized to evaluate the cell-killing effects of novel treatments on cancer cells in vitro

  10. Effect of caffeine on gamma-ray induced G2 arrest in well-synchronized Chinese hamster ovary cells in vitro

    International Nuclear Information System (INIS)

    Masunaga, Shin-ichiro; Keng, P.C.


    G1-rich cells were separated from exponentially growing asynchronous cultured Chinese hamster ovary (CHO-K1) cells by centrifugal elutriation and a Coulter Counter. The G1-rich cells were incubated in medium that contained hydroxyurea (HU) to kill S phase cells and obtain the purest G1/S boundary cells possible. The HU-treated cells were washed, and were again incubated, in medium without HU, to allow these well-synchronized G1/S boundary cells to progress to S and G2/M phases. At various times after release from G1/S boundary, 4 Gy of gamma-ray and/or caffeine was administered to the cells. Eight hours after the removal of HU, cell-cycle analysis was performed with a flow cytometer. G2 arrest induced by gamma-rays was clearly shown when radiation was given earlier than 6.5 hours after HU removal. G2 arrest induced by radiation given 0.5-6.5 hours after HU removal was reduced by caffeine treatment given 6.0-6.5 hours after HU removal. Caffeine released radiation-induced G2 arrest when the radiation was given before the cultured cells entered G2/M phase and when caffeine was applied to the irradiated cells at the time when G1/S boundary cells not treated by radiation or with caffeine entered G2/M phase. Our method of centrifugal elutriation combined with incubation with HU was useful for isolating pure G1/S boundary cells from in vitro asynchronous cultures. (author)

  11. Last glacial tephra layers in the Talos Dome ice core (peripheral East Antarctic Plateau), with implications for chronostratigraphic correlations and regional volcanic history (United States)

    Narcisi, Biancamaria; Petit, Jean Robert; Langone, Antonio


    Tephra isochrons offer considerable potential for correlating diverse palaeoarchives and highlighting regional climatic differences. They are especially useful when applied to polar ice records encompassing the last glacial, as these clearly portray the pronounced millennial-scale climate variability that characterised this period. Here we present the continuous record of primary fallout tephra layers in the East Antarctic Talos Dome ice core (72°49‧S, 159°11‧E), developed upon examination of the core sections spanning the glacial period 16.5 to 71 ka. A total of ca. 45 discrete tephra deposits precisely positioned stratigraphically relative to the temperature record for the core and dated using the AICC2012 timescale, were identified. Quantitative grain size, particle morphology, major and trace element composition using Coulter Counter, SEM, EPMA-WDS, and LA-ICP-MS analytical methods were studied as diagnostic features for tephra characterisation. The tephrostratigraphic framework provides a reference for future precise comparison between ice and sediment sequences across the Antarctic continent. Indeed, several potential markers characterised by distinct volcanic glass geochemistry and/or particular stratigraphic location (e.g., a 17.6-ka ash layer deposited during the well-known major acidity event) are now available for the direct linkage of palaeoclimatic archives. The Talos Dome tephra sequence, dominated by mid-distal pyroclastic products from the nearby Northern Victoria Land volcanoes, also represents the most comprehensive and best time-constrained record of regional Antarctic volcanism yet developed. It documents nearly continuous sustained explosive activity during the considered time interval and, combined with previous ice-core tephra results for the last and the current interglacial periods, suggests progressive compositional shift through time.

  12. Role of intracellular calcium in cellular volume regulation

    International Nuclear Information System (INIS)

    Wong, S.M.; Chase, H.S. Jr.


    We investigated the role of intracellular calcium in epithelial cell volume regulation using cells isolated from the toad urinary bladder. A suspension of cells was prepared by treatment of the bladder with collagenase followed by ethyleneglycol-bis(beta-aminoethylether)-N,N'-tetraacetic acid. The cells retained their ion-transporting capabilities: ouabain (1 mM) and amiloride (10 microM) inhibited cellular uptake of 86 Rb and 22 Na, respectively. Using a Coulter counter to measure cellular volume, we found that we could swell cells either by reducing the extracellular osmolality or by adding the permeant solute urea (45 mM) isosmotically. Under both conditions, cells first swelled and then returned to their base-line volume, in spite of the continued presence of the stimulus to swell. Volume regulation was inhibited when cells were swelled at low extracellular [Ca] (100 nM) and was retarded in cells preloaded with the calcium buffer quin 2. Swelling increased the intracellular free calcium concentration ([Ca]i), as measured by quin 2 fluorescence: [Ca]i increased 35 +/- 9 nM (n = 6) after hypotonic swelling and 42 +/- 3 nM (n = 3) after urea swelling. Reducing extracellular [Ca] to less than 100 nM prevented the swelling-induced increase in [Ca]i, suggesting that the source of the increase in [Ca]i was extracellular. This result was confirmed in measurements of cellular uptake of 45Ca: the rate of uptake was significantly higher in swollen cells compared with control (1.1 +/- 0.2 vs. 0.4 +/- 0.1 fmol . cell-1 X 5 min-1). Our experiments provide the first demonstration that cellular swelling increases [Ca]i. This increase is likely to play a critical role in cellular volume regulation

  13. A perfluorobutylpentane (F4H5)-based solution for the removal of residual emulsified silicone oil. (United States)

    Chan, Yau Kei; Cheng, Ho Ching; Wu, Jing; Tang, Yuk Heng Matthew; Chan, San To; Wong, David; Shum, Ho Cheung


    The emulsification of silicone oil (SO) is associated with many complications. In this study, we investigate a new SO solvent, perfluorobutylpentane (F4H5) with 1% by volume of perfluorinated polyethers-polyethylene glycol-perfluorinated polyethers (PFPE-PEG-PFPE) triblock copolymer, for removing emulsified droplets. An in vitro 3D printed model eye chamber was used to evaluate the efficiency of the three test liquids in removing SO droplets, namely saline, F4H5 and F4H5 with surfactant PFPE-PEG-PFPE. The numbers of SO droplets were quantified using a Coulter Counter. The stability of double emulsion formed was tested with a fluidic device based on electro-coalescence. Two retinal cell lines were used to test the biocompatibility of the liquids. The mean number of droplets remaining in the eye chamber after rinsing with a solution of F4H5 with surfactant was 13 315 ± 4620/ml compared to saline (23 460 ± 7595/ml; p < 0.05). The double emulsion was found to be highly stable. The biocompatibility of F4H5 and the surfactant was similar to that of the saline control. By adding a small amount of surfactant, the resultant F4H5 solution is able to promote double emulsification and remove more SO droplets. Although further in vivo safety studies are necessary before clinical trials, the result of our study suggests that F4H5 with surfactant is a promising Rinsing Solution for removing emulsified droplets. This work therefore translates a well-known phenomenon in emulsion science to tackle the emulsification problem observed in the routine vitreoretinal surgery. © 2017 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.

  14. Classification of biological cells using a sound wave based flow cytometer (United States)

    Strohm, Eric M.; Gnyawali, Vaskar; Van De Vondervoort, Mia; Daghighi, Yasaman; Tsai, Scott S. H.; Kolios, Michael C.


    A flow cytometer that uses sound waves to determine the size of biological cells is presented. In this system, a microfluidic device made of polydimethylsiloxane (PDMS) was developed to hydrodynamically flow focus cells in a single file through a target area. Integrated into the microfluidic device was an ultrasound transducer with a 375 MHz center frequency, aligned opposite the transducer was a pulsed 532 nm laser focused into the device by a 10x objective. Each passing cell was insonfied with a high frequency ultrasound pulse, and irradiated with the laser. The resulting ultrasound and photoacoustic waves from each cell were analyzed using signal processing methods, where features in the power spectra were compared to theoretical models to calculate the cell size. Two cell lines with different size distributions were used to test the system: acute myeloid leukemia cells (AML) and melanoma cells. Over 200 cells were measured using this system. The average calculated diameter of the AML cells was 10.4 +/- 2.5 μm using ultrasound, and 11.4 +/- 2.3 μm using photoacoustics. The average diameter of the melanoma cells was 16.2 +/- 2.9 μm using ultrasound, and 18.9 +/- 3.5 μm using photoacoustics. The cell sizes calculated using ultrasound and photoacoustic methods agreed with measurements using a Coulter Counter, where the AML cells were 9.8 +/- 1.8 μm and the melanoma cells were 16.0 +/- 2.5 μm. These results demonstrate a high speed method of assessing cell size using sound waves, which is an alternative method to traditional flow cytometry techniques.

  15. Particle size distribution and column efficiency. An ongoing debate revived with 1.9μm Titan-C18 particles. (United States)

    Gritti, Fabrice; Bell, David S; Guiochon, Georges


    The mass transfer mechanism in four prototype columns (2.1 and 3.0×50mm, 2.1 and 3.0×100mm) packed with 1.9μm fully porous Titan-C18 particles was investigated by using two previously reported home-made protocols. The first one was used to measure the eddy dispersion HETP of these new columns, the second one to estimate their intrinsic (corrected for HPLC system contribution) HETPs. Titan particles are fully porous particles with a narrow particle size distribution (RSD of 9.2%). The mean Sauter diameter (dSauter=2.04μm) was determined from Coulter counter measurements on the raw silica material (before C18 derivatization) and in the absence of a dispersant agent (Triton X-100) in a 2% NaCl electrolyte solution. The results show that these RPLC Titan columns have intrinsic minimum reduced HETPs ranging from 1.7 to 1.9 and generate up to 290,000 plates per meter. The 3.0mm i.d. columns are more efficient than the 2.1mm i.d. ones and short columns are preferred to minimize efficiency losses due to frictional heating at high speeds. This work also revealed that (1) the lowest h values of the Titan columns are observed at low reduced velocities (νopt=5); (2) this is due to the unusually small diffusivity of analytes across the porous Titan-C18 particles; and (3) the Titan columns are not packed more uniformly than conventional columns packed with fully porous particles. Earlier and recent findings showing that the PSD has no direct physical impact on eddy dispersion and column efficiency are confirmed by these results. Copyright © 2014 Elsevier B.V. All rights reserved.

  16. Cellular immunity and levels of parathyroid hormone in uremic patients receiving hemodialysis. (United States)

    Griveas, Ioannis; Visvardis, G; Papadopoulou, D; Mitsopoulos, E; Kyriklidou, P; Manou, E; Meimaridou, D; Ginikopoulou, E; Sakellariou, G; Fleva, A; Zilidou, R; Paulitou, A


    The clinical relevance of altered lymphocyte function and the possible relation with uremic toxins, such as parathyroid hormone (PTH) is not well understood. We studied the changes in cellular immunity in patients in hemodialysis (HD) therapy and examined the relationship between T-lymphocyte function and plasma levels of PTH. Thirty-four patients (14 male) were enrolled in this study (mean age: 63.20 +/- 10.01 years, M +/- SD, 12 h/week HD). Our study population was divided into two groups: 18 patients with increased levels of PTH and 16 patients with normal levels of PTH. Lymphocyte subsets (CD2+, CD3+, CD3+/4+, CD3+/8+, CD19+, CD3-/16+56+, CD4/CD8 ratio) were quantitated in both groups using monoclonal antibodies (Immunotech, Coulter) and flow cytometric analysis. Following analysis of variance (ANOVA) testing was performed to test differences between groups (SPSS version 10). A significant increase of CD2 was noticed in patients with increased levels of PTH (84.8 +/- 5.5 vs. 79.8 +/- 4, p<0.05). The CD3 population was also increased in patients with elevated PTH (72 +/- 8.6 vs. 68 +/- 9.2, p=NS). This group of patients had also significantly increased levels of CD3/8 (44.8 +/- 9.8 vs. 37.1 +/- 5.8, p<0.05). The CD4/CD8 ratio levels were higher in patients with elevated PTH compared with those who had normal PTH (2.2 +/- 1.5 vs. 1.5 +/- 0.8, p=NS). The elevated level of PTH seems to affect the lymphocyte function and is associated with changes in cellular immunity in the hemodialysis population. Our study is in progress in order to enlarge our study population and collect more data, which will lead us to more solid conclusions.

  17. Single Cell Electroporation Method for Mammalian CNS Neurons in Organotypic Slice Cultures (United States)

    Uesaka, Naofumi; Hayano, Yasufumi; Yamada, Akito; Yamamoto, Nobuhiko

    Axon tracing is an essential technique to study the projection pattern of neurons in the CNS. Horse radish peroxidase and lectins have contributed to revealing many neural connection patterns in the CNS (Itaya and van Hoesen, 1982; Fabian and Coulter, 1985; Yoshihara, 2002). Moreover, a tracing method with fluorescent dye has enabled the observation of growing axons in living conditions, and demon strated a lot of developmental aspects in axon growth and guidance (Harris et al., 1987; O'Rourke and Fraser, 1990; Kaethner and Stuermer, 1992; Halloran and Kalil, 1994; Yamamoto et al., 1997). More recently, genetically encoded fluores cent proteins can be used as a powerful tool to observe various biological events. Several gene transfer techniques such as microinjection, biolistic gene gun, viral infection, lipofection and transgenic technology have been developed (Feng et al., 2000; Ehrengruber et al., 2001; O'Brien et al., 2001; Ma et al., 2002; Sahly et al., 2003). In particular, the electroporation technique was proved as a valuable tool, since it can be applied to a wide range of tissues and cell types with little toxicity and can be performed with relative technical easiness. Most methods, including a stand ard electroporation technique, are suitable for gene transfer to a large number of cells. However, this is not ideal for axonal tracing, because observation of individ ual axons is occasionally required. To overcome this problem, we have developed an electroporation method using glass micropipettes containing plasmid solutions and small current injection. Here we introduce the method in detail and exemplified results with some example applications and discuss its usefulness.

  18. La complejidad de las organizaciones: aproximación a un modelo teórico

    Directory of Open Access Journals (Sweden)

    Gerardo J. Zapata Rotundo


    Full Text Available Este artículo propone un modelo teórico sobre la complejidad de las organizaciones, teniendo como marco de referencia los constructos complejidad cognitiva y complejidad relacional. La complejidad relacional (Hall et al., 1967; Boisot y Child, 1999; Hall, 1996; Robbins y Coulter, 2002, se vincula con los procesos de diferenciación e integración que se dan en las organizaciones, y se explica fundamentalmente a través del comportamiento y gestión de las variables de diseño organizativo. Por su parte, la complejidad cognitiva (Perrow, 1970; Boisot y Child, 1999; Peris et al., 2001, está asociada con la dificultad de comprender y llevar a cabo los aspectos más esenciales del trabajo realizado en la organización; de ahí, la presencia de diferentes tipos de trabajo con distintos grados de dificultad y variedad (naturaleza del trabajo. El modelo sirve de base a tres proposiciones que se señalan en las consideraciones finales de la investigación, donde se plantean un conjunto de relaciones entre sus elementos constituyentes: tipos básicos de trabajo, variables de diseño (centralización y formalización, complejidad cognitiva y relacional, estilo de liderazgo y forma organizativa que adopta la empresa. Por último, se pretende con el modelo propuesto contribuir al esfuerzo de seguir comprendiendo el diseño de las organizaciones y su comportamiento, y, por tanto, a favor de la formulación de nuevas soluciones a sus problemas.

  19. Radioprotection conferred by dextran sulfate given before irradiation in mice

    International Nuclear Information System (INIS)

    Ross, W.M.; Peeke, J.


    Dextran sulfate (DS) has been observed to cause mobilization (fivefold) of hemopoietic stem cells (HSC) and leukocytes, primarily lymphocytes, into the peripheral blood of mice within 2-3 h after intraperitoneal (i.p.) injection. This effect was dose dependent and was prolonged for several hours when the high-molecular-weight version DS500 (500,000 daltons) was used. When DS500 was given 1-3 days before irradiation, hemopoietic recovery was markedly enhanced. Postirradiation injection was ineffective. By ten days after irradiation (7.0 Gy), the number of endogenous spleen colonies (CFUs) and the splenic mass were much larger if DS pretreatment had been given. This effect was dependent on the dose of DS500 and on the time administered, 60 mg/kg producing a maximal effect when given three days before irradiation. DS500 caused a transient anaphylactoid shock, however, in most mice--mild at low doses but potentially lethal at doses above 40 mg/kg (10% mortality within 1-3 days after 60 mg/kg). The following results were obtained with 50 mg/kg, a compromise dose causing minimal mortality (3%) given three days before irradiation. Reticulocyte reappearance was earlier in irradiated mice given DS500, indicating earlier erythropoietic recovery. Some of these reticulocytes were resistant to lysing agents, so their appearance could be detected using the Coulter electronic cell counter, as well as in stained blood smears. The 30-day mortality due to bone marrow failure after irradiation was significantly decreased in DS-treated mice below 9.5 Gy, and the LD50/30 was increased by 0.5 Gy. This study shows that dextran sulfate exerts a radioprotective influence on the hemopoietic system and hence survival when administered prophylactically

  20. Clinical Interpretation of Elevated Concentrations of Cardiac Troponin T, but Not Troponin I, in Nursing Home Residents. (United States)

    Cardinaels, Eline P M; Daamen, Mariëlle A M J; Bekers, Otto; ten Kate, Joop; Niens, Marijke; van Suijlen, Jeroen D E; van Dieijen-Visser, Marja P; Brunner-La Rocca, Hans-Peter; Schols, Jos M G A; Mingels, Alma M A


    Cardiac troponins T (cTnT) and I (cTnI) are the preferred biomarkers to detect myocardial damage. The present study explores the value of measuring cardiac troponins (cTn) in nursing home residents, by investigating its relation to heart failure and 1-year mortality using 1 cTnT and 2 cTnI assays that are widely used in clinical practice. All participants underwent extensive clinical examinations and echocardiographic assessment for the diagnosis of heart failure. cTn was measured using high-sensitive (hs)- cTnT (Roche), hs-cTnI (Abbott), and sensitive cTnI (Beckman) assays. The glomerular filtration rate was estimated (eGFR) using serum creatinine and cystatin C concentrations. Data on all-cause mortality were collected at 1-year follow-up. Participants were 495 long-term nursing home residents, older than 65 years, of 5 Dutch nursing home organizations. Median (IQR) concentrations were 20.6 (17.8-30.6), 6.8 (4.1-12.5), and 4.0 (2.0-8.0) ng/L for hs-cTnT, hs-cTnI, and cTnI, respectively. In total, 79% had elevated hs-cTnT concentrations, whereas only 9% and 5% of hs-cTnI and cTnI concentrations were elevated. Most important and independent determinants for higher hs-cTnT and hs-cTnI concentrations were heart failure and renal dysfunction. Whereas both heart failure (odds ratio [OR] 3.4) and eGFR lower than 60 mL/min/1.73 m(2) (OR 3.6) were equal contributors to higher hs-cTnT concentrations (all P cardiac health, hs-cTnT but not hs-cTnI concentrations were elevated in almost all aged nursing home residents, questioning the use of the current diagnostic cutoff in elderly with high comorbidity. Nonetheless, measuring cardiac troponins, especially hs-cTnT, had a promising role in assessing future risk of mortality. Copyright © 2015 AMDA – The Society for Post-Acute and Long-Term Care Medicine. Published by Elsevier Inc. All rights reserved.

  1. Prevalence of Age-Related Macular Degeneration in Australia: The Australian National Eye Health Survey. (United States)

    Keel, Stuart; Xie, Jing; Foreman, Joshua; van Wijngaarden, Peter; Taylor, Hugh R; Dirani, Mohamed


    Age-related macular degeneration (AMD) is a leading cause of irreversible blindness among the elderly population globally. Currently, knowledge of the epidemiology of AMD in Australia remains scarce because of a paucity of recent population-based data. To examine the prevalence of AMD in Australia. In this population-based, cross-sectional survey performed from March 11, 2015, to April 18, 2016, a sample of 3098 nonindigenous Australians 50 years and older and 1738 indigenous Australians 40 years and older from 30 geographic areas across Australia were examined. Any AMD, early AMD, intermediate AMD, and late AMD graded according to the Beckman clinical classification system. A total of 4836 individuals were examined, including 3098 nonindigenous Australian (64.1%; 58.9% female vs 41.1% male; age range, 40-92 years; mean [SD] age, 55.0 [10.0] years) and 1738 indigenous Australians (35.9%; 53.6% female vs 46.4% male; age range, 50-98 years; mean [SD] age, 66.6 [9.7] years). A total of 4589 (94.9%, 2946 nonindigenous and 1643 indigenous) participants had retinal photographs in at least 1 eye that were gradable for AMD. The weighted prevalence of early AMD was 14.8% (95% CI, 11.7%-18.6%) and of intermediate AMD was 10.5% (95% CI, 8.3%-13.1%) among nonindigenous Australians. In indigenous Australians, the weighted prevalence of early AMD was 13.8% (95% CI, 9.7%-19.3%) and of intermediate AMD was 5.7% (96% CI, 4.7%-7.0%). Late AMD was found in 0.96% (95% CI, 0.59%-1.55%) of nonindigenous participants (atrophic, 0.72%; neovascular, 0.24%). The prevalence of late AMD increased to 6.7% in participants 80 years or older and was higher in men (1.4% vs 0.61%, P = .02). Only 3 (0.17% [95% CI, 0.04%-0.63%]) indigenous participants had late (atrophic) AMD. Age-related macular degeneration was attributed as the main cause of vision loss (Australia.

  2. Perfil de aptidão cardiorrespiratória e metabólica em bailarinos profissionais

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    Paulo Roberto Santos Silva


    Full Text Available O principal objetivo deste estudo foi analisar aspectos cardiorrespiratórios e metabólicos e as alterações provocadas pelo treinamento específico de dança em um grupo de 16 bailarinos de balé profissional, modalidade clássico, sendo oito mulheres e oito homens, com média de idade de 18,2 ± 3,8 anos e 26,2 ± 4,5 anos, respectivamente. Todos foram submetidos a teste máximo em esteira rolante utilizando-se o protocolo de Bruce. Foi utilizado, na análise das respostas respiratórias e metabólicas, o sistema computadorizado Metabolic Measurement Cart da Beckman. Os seguintes resultados foram obtidos entre o grupo de balé vs. o grupo controle masculino: VO2 máx. - 46 ± 4 vs. 43 ±; FC máx. - 194 ± 12 vs. 202 ± 11bpm; V E máx. - 112 ± 16 vs. 123 ± 18L.min-1; VO2-LA - 35 ± 4 vs. 26 ± (p < 0,01; FC-LA - 169 ± 18 vs. 163 ± 15 bpm. Grupo de balé vs. grupo controle feminino: VO2 máx. - 39 ± 6 vs. 35 ±; FC máx. - 197 ± 10 vs. 201 ± 6bpm; V E máx. - 72 ± 9 vs. 81 ± 6L.min-1; VO2-LA - 26 ± 4 vs. 27 ±; FC-LA - 164 ± 10 vs. 176 ± 17bpm. Conclusões: 1 a rotina específica de dança parece não gerar estímulo suficiente para aprimorar a aptidão cardiorrespiratória e metabólica dos bailarinos e 2 sugere-se condicionamento físico adicional ao treinamento de balé.

  3. Excreção urinária de 17-Cetoesteroides neutros no cavalo normal e no cavalo castrado

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    Fernando Ubatuba


    Full Text Available Total urinary neutral 17-steroids were determined in normal and in castrated horses. One liter of a 15-26 hours urine collection was hydrolysed by refluxing with 10% HC1 (v/v for ten minutes and extracted with peroxyde-free ethyl ether. The extract was purified by washing with saturated NaHCO³ and KOH solutions. One half of the crude neutral fraction was fractionated with Girard's "T" reagent . The Zimmermann reaction was performed both in the ketonic and in the crude neutral extracts, using alcoholic 2.5N KOH and a 60 minutes period for the colour development in the dark. Optical density measuments were made in a grating Coleman Universal Spectrophotometer at 420 mµ and 520mµ; for the crude neutral fraction a colour correction equation was applied. The aliquot fraction used for colorimety was adjusted for keeping optical density measurements within the range 0.2 to 0.7. Androsterone (mp. 184-184.5°C with an absorption maximum at 290.5 mµ (Beckman Model DU Spectrophotometer was used as a reference standard. Table I, ilustrates the results obtained. At the 0.05 probability level there is a significant difference among castrated and normal group means (Fischer's "t" test. when were used the data obtained from the ketonic fractions; in spite of the use of a colour correction applied for inespecific chromogens, the same results could not be obtained with the crude neutral fractions, Since Girard's reagent fractionation is generaly accepted as the best method for correcting the inespecific chromogen interference in the determination of the 17-ketosteroids by the Zimmermann reaction, we emphasize the value of the results obtained with the ketonic fractions. From these results it appears, as occurs in others mammals, that castrated horses show a lower level of urinary 17-ketosteroids excretion than the normal horses. The significance of the horse testis contribution for the neutral urinary steroid metabolites is discussed. Since horse urine has a

  4. [Studies on the mineral contents of some trematodes (United States)

    Lee, Sang Whon


    The mineral contents of the adult Clonorchis sinensis from rabbits and human were measured, and its qualitative and semi-quantitative analyses were studied with 3.4 meter Ebert-Grating spectrograph, and its quantitative analyses were studied with Beckman DU spectrophotometer. The adult Clonorchis sinensis used in this study were divided into two groups, the first group was collected from the bile passage of the man (C. sinensis from man), the second group was collected from the bile passage of the rabbit(C. sinensis from rabbit). Simultaneously, spectrographic and photometric analyses were also performed on the adult worms of Paragonimus westermani. Furthermore, comparative spectrographic analyses of the trace elements were carried out on the C. sinensis from man, C. sinensis from rabbit, Fasciola hepatica, Eurytrema pancreaticum, and Paragonimus westermani, and the approximate contents of the trace elements of the above trematodes were compared with that of their host tissues and biles. The results obtained were as follows: 1. In the spectrographic analyses of C. sinensis from man, sodium, phosphorus, potassium, copper, calcium, aluminum, iron, and magnesium were detected predominantly, and zinc, titanium, silicone, lead, manganese, barium, cromium, molybdenum, and silver were detected as trace elements. 2. In the differences of level of the minerals in these two C. sinensis, copper level of C. sinensis from man was higher than that of C. sinensis from rabbit, while calcium level of the former worm was higher than that of the latter worm. The trace level of lead, molybdenum, and silver were acertained in the former, but latter were not detected. 3. The contents of the minerals showed the characteristic features in each trematodes: the mineral of flukes in each host were much more than that of the others; such as copper in C. sinensis from man, calcium in the C. sinensis from rabbit, and silicone in the P. westermani. The vanadium was detected in the F. hepatica, E

  5. A high-temperature catalytic oxidation method for the determination of dissolved organic carbon in seawater: analysis and improvement (United States)

    Suzuki, Yoshimi; Tanoue, Eiichiro; Ito, Hiroshi


    The high-temperature catalytic oxidation (HTCO) method for the determination of dissolved organic carbon in seawater, reported by SUGIMURA and SUZUKI (1988, Marine Chemistry, 24, 105-131) has been improved to provide more valid data on the levels of dissolved organic carbon (DOC). The improvements are as follows. (1) An open-close valve, made from an alloy of iron, molybdenum, nickel and chromium and without a silicone septum, is used as the injection port. (2) Copper oxide and sulfix (mixture of AgO and CoO) are packed with a 3% platinum (Pt) catalyst into the combustion tube for the complete elimination of halogenated and sulfur compounds. (3) The top of the catalyst is covered with Pt gauze to prevent the Pt catalyst from flying upwards. (4) A glass water-trap, containing phosphoric acid and silver nitrate in solution, is connected just below the outlet of the combustion tube. (5) A high-sensitivity, infra-red gas analyser (Beckman model 880) is used. The blank value for the system is checked using water prepared by passage through the improved HTCO system. The total blank value (system blank plus water blank) ranges from 15 to 30 μM C. Of the total blank volume, the blank value for the system which was examined using water collected from the HTCO system, was less than 3 μM C. The greatest contributions to the blank value in this machine are made by the organic matter originally present in deionized water. The oxidation efficiency of the catalyst with different levels of Pt was examined for measurements of DOC. Although there is little difference in detected concentrations of DOC from the surface to deep waters with levels of 1.5, 3 and 5% Pt in the catalyst, the alumina impregnated with higher levels of Pt is much better for the complete oxidation of the DOC in seawater. The size of the combustion tube, the quality of the catalyst and the uniform distribution of the furnace temperature are key elements in determining the oxidative capacity of the system. It

  6. Content Of 2,4-D-14C Herbicide Residue In Water And Soil Of Irrigated Rice Field System

    International Nuclear Information System (INIS)

    Chairul, Sofnie M.; Djabir, Elida; Magdalena, Nelly


    The investigation of 2,4-D exp.-14C herbicide residue in water and soil of irrigated rice field system was carried out. Rice plant and weeds (Monochoria vaginalis Burn. F. Presl) were planted in 101 buckets using two kinds of soil condition, I.e. normal soil and 30 % above normal compact soil. After one week planting, the plants were sprayed with 1 u Ci of 2,4-D exp.-14C and 0,4 mg non labeled 2,4-D. The herbicide residue content was determined 0, 2, 4, 8 and 10 weeks after spraying with 2,4-D herbicide. The analysis was done using Combustion Biological Oxidizer merk Harvey ox-400, and counted with Liquid Scintillation Counter merk Beckman model LS-1801. The results indicates that the herbicide contents in water and soil decrease from the first spraying with herbicide until harvest herbicide Residue content in water after harvest was 0.87 x 10 exp.-6 ppm for soil normal condition, and 0.59 x 10 exp.-6 pm for the soil 30 % up normal condition, while herbicide content in soil was 1.54 x 10 exp.-6 ppm for soil normal condition and 1.48 x 10 exp.-6 ppm for soil 30 % up normal. 2,4-D herbicide residue content in rice after harvest was 0.27 x 10 exp.-6 ppm for normal soil condition and 0.25 x 10 exp.-6 ppm for the soil 30 % up normal. 2,4-D herbicide residue content in roots and leaves of weeds after harvest were respectively 0.29 x 10 exp.-6 ppm and 0.18 x 10 exp.-6 for normal soil condition, while for 30 % up normal soil were 0.25 x 10 exp.-5 ppm and 0.63 x 10 exp.-7 ppm. This result indicates that there is no effect pollution to surrounding area, because the herbicide content is still bellow the allowed detection limit, 0.05 ppm

  7. Making the diagnosis of Sjögren’s syndrome in patients with dry eye

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    Beckman KA


    Full Text Available Kenneth A Beckman,1,2 Jodi Luchs,3,4 Mark S Milner5,6 1Comprehensive EyeCare of Central Ohio, Westerville, OH, 2The Ohio State University, Columbus, OH, 3Hofstra University School of Medicine, Hempstead, NY, 4South Shore Eye Care, Wantagh, NY, 5The Eye Center of Southern Connecticut, PC, Hamden, CT, 6Yale University School of Medicine, New Haven, CT, USA Abstract: Sjögren’s syndrome (SS is a chronic and progressive systemic autoimmune disease that often presents initially with symptoms of dry eye and dry mouth. Symptoms are often non-specific and develop gradually, making diagnosis difficult. Patients with dry eye complaints warrant a step-wise evaluation for possible SS. Initial evaluation requires establishment of a dry eye diagnosis using a combination of patient questionnaires and objective ocular tests, including inflammatory biomarker testing. Additional work-up using the Schirmer test and tear film break-up time can differentiate between aqueous-deficient dry eye (ADDE and evaporative dry eye. The presence of ADDE should trigger further work-up to differentiate between SS-ADDE and non-SS-ADDE. There are numerous non-ocular manifestations of SS, and monitoring for SS-related comorbid findings can aid in diagnosis, ideally in collaboration with a rheumatologist. The clinical work-up of SS can involve a variety of tests, including tear function tests, serological tests for autoantibody biomarkers, minor salivary gland and lacrimal gland biopsies. Examination of classic SS biomarkers (SS-A/Ro, SS-B/La, antinuclear antibody, and rheumatoid factor is a convenient and non-invasive way of evaluating patients for the presence of SS, even years prior to confirmed diagnosis, although not all SS patients will test positive, particularly those with early disease. Recently, newer biomarkers have been identified, including autoantibodies to salivary gland protein-1, parotid secretory protein, and carbonic anhydrase VI, and may allow for earlier

  8. Single-beam integrating sphere spectrophotometer for reflectance and transmittance measurements versus angle of incidence in the solar wavelength range on diffuse and specular samples (United States)

    Nostell, Per; Roos, Arne; Rönnow, Daniel


    substrates. The results are also compared to the results of a double-beam Beckman integrating sphere for near normal angles of incidence and Fresnel calculations. The results in this article show that good agreement is obtained between results from the different instruments if, and only if, proper evaluation procedures are applied to the measured signals.

  9. Antigen Expression on Blast Cells and Hematological Parameters at Presentation in Acute Lymphoblastic Leukemia Patients

    International Nuclear Information System (INIS)

    Naeem, S.; Bukhari, M. H.


    Objective: To analyze the expression of various antigens on the leukemic blasts and to determine the hematological parameters, in Acute Lymphoblastic Leukemia (ALL) patients at presentation. Study Design: Observational study. Place and Duration of Study: King Edward Medical University, Lahore and Hameed Latif Hospital, Lahore, from February 2013 to March 2014. Methodology: A total of 50 newly diagnosed and untreated patients of ALL were selected from Mayo Hospital and Hameed Latif Hospital. These patients included both genders and all age groups. Hemoglobin, total leukocyte count and platelet count were determined on hematology analyser-Sysmex-Kx-2I. Blast cell percentage was estimated on Giemsa stained blood smears. Immuno phenotyping was done on bone marrow samples by 5 colour flow cytometery on Beckman Counter Navious Flow cytometer. An acute leukemia panel of 23 antibodies was used. The data was entered and analyzed in SPSS version 22. Results: Of the 50 ALL patients, 36 (72 percentage) were B-ALL and 14 (28 percentage) T-ALL. There were 18 (36 percentage) children and 32 (64 percentage) adults. T-ALL included 22 percentage of the childhood and 31 percentage of the adult cases. Immuno phenotypic analysis showed that CD19, CD79a and CD20 were B-lineage specific markers whereas cCD3, CD3 and CD5 were T-lineage specific. CD10 was the most sensitive marker for B-ALL and CD7 was the most sensitive marker of T-ALL. TdT was expressed in 92 percentage B-ALL and 71 percentage T-ALL cases, CD34 in 58 percentage and 43 percentage cases and CD45 in 83 percentage and 100 percentage respectively. High leukocyte count (> 50 x 109/L) was present in 58 percentage cases. Hemoglobin was < 10 g/dl in 74 percentage patients and platelet count was below 20 x 109/Lin 12 percentage patients. Leukocyte count, hemoglobin, platelet count and blast cell percentage did not show a significant difference in the two ALL immuno types. Conclusion: The frequency of T-ALL is higher in childhood

  10. The Oxygen Paradox, the French Paradox, and age-related diseases. (United States)

    Davies, Joanna M S; Cillard, Josiane; Friguet, Bertrand; Cadenas, Enrique; Cadet, Jean; Cayce, Rachael; Fishmann, Andrew; Liao, David; Bulteau, Anne-Laure; Derbré, Frédéric; Rébillard, Amélie; Burstein, Steven; Hirsch, Etienne; Kloner, Robert A; Jakowec, Michael; Petzinger, Giselle; Sauce, Delphine; Sennlaub, Florian; Limon, Isabelle; Ursini, Fulvio; Maiorino, Matilde; Economides, Christina; Pike, Christian J; Cohen, Pinchas; Salvayre, Anne Negre; Halliday, Matthew R; Lundquist, Adam J; Jakowec, Nicolaus A; Mechta-Grigoriou, Fatima; Mericskay, Mathias; Mariani, Jean; Li, Zhenlin; Huang, David; Grant, Ellsworth; Forman, Henry J; Finch, Caleb E; Sun, Patrick Y; Pomatto, Laura C D; Agbulut, Onnik; Warburton, David; Neri, Christian; Rouis, Mustapha; Cillard, Pierre; Capeau, Jacqueline; Rosenbaum, Jean; Davies, Kelvin J A


    A paradox is a seemingly absurd or impossible concept, proposition, or theory that is often difficult to understand or explain, sometimes apparently self-contradictory, and yet ultimately correct or true. How is it possible, for example, that oxygen "a toxic environmental poison" could be also indispensable for life (Beckman and Ames Physiol Rev 78(2):547-81, 1998; Stadtman and Berlett Chem Res Toxicol 10(5):485-94, 1997)?: the so-called Oxygen Paradox (Davies and Ursini 1995; Davies Biochem Soc Symp 61:1-31, 1995). How can French people apparently disregard the rule that high dietary intakes of cholesterol and saturated fats (e.g., cheese and paté) will result in an early death from cardiovascular diseases (Renaud and de Lorgeril Lancet 339(8808):1523-6, 1992; Catalgol et al. Front Pharmacol 3:141, 2012; Eisenberg et al. Nat Med 22(12):1428-1438, 2016)?: the so-called, French Paradox. Doubtless, the truth is not a duality and epistemological bias probably generates apparently self-contradictory conclusions. Perhaps nowhere in biology are there so many apparently contradictory views, and even experimental results, affecting human physiology and pathology as in the fields of free radicals and oxidative stress, antioxidants, foods and drinks, and dietary recommendations; this is particularly true when issues such as disease-susceptibility or avoidance, "healthspan," "lifespan," and ageing are involved. Consider, for example, the apparently paradoxical observation that treatment with low doses of a substance that is toxic at high concentrations may actually induce transient adaptations that protect against a subsequent exposure to the same (or similar) toxin. This particular paradox is now mechanistically explained as "Adaptive Homeostasis" (Davies Mol Asp Med 49:1-7, 2016; Pomatto et al. 2017a; Lomeli et al. Clin Sci (Lond) 131(21):2573-2599, 2017; Pomatto and Davies 2017); the non-damaging process by which an apparent toxicant can activate biological signal

  11. The effect of steam sterilization on the accuracy of spring-style mechanical torque devices for dental implants

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    Mahshid M


    Full Text Available Minoo Mahshid,1 Aboulfazl Saboury,1 Ali Fayaz,1 Seyed Jalil Sadr,1 Friedrich Lampert,2 Maziar Mir2,31Department of Prosthodontics, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2Department of Conservative Dentistry, Aachen RWTH Hospital, Aachen, Germany; 3Beckman Laser Institute, UCI, Irvine, CA, USABackground: Mechanical torque devices (MTDs are one of the most commonly recommended devices used to deliver optimal torque to the screw of dental implants. Recently, high variability has been reported about the accuracy of spring-style mechanical torque devices (S-S MTDs. Joint stability and survival rate of fixed implant supported prosthesis depends on the accuracy of these devices. Currently, there is limited information on the steam sterilization influence on the accuracy of MTDs. The purpose of this study was to assess the effect of steam sterilization on the accuracy (±10% of the target torque of spring-style mechanical torque devices for dental implants.Materials and methods: Fifteen new S-S MTDs and their appropriate drivers from three different manufacturers (Nobel Biocare, Straumann [ITI], and Biomet 3i [3i] were selected. Peak torque of devices (5 in each subgroup was measured before and after autoclaving using a Tohnichi torque gauge. Descriptive statistical analysis was used and a repeated-measures ANOVA with type of device as a between-subject comparison was performed to assess the difference in accuracy among the three groups of spring-style mechanical torque devices after sterilization. A Bonferroni post hoc test was used to assess pairwise comparisons.Results: Before steam sterilization, all the tested devices stayed within 10% of their target values. After 100 sterilization cycles, results didn't show any significant difference between raw and absolute error values in the Nobel Biocare and ITI devices; however the results demonstrated an increase of error values in the 3i group (P < 0.05. Raw error

  12. Update on value of the anion gap in clinical diagnosis and laboratory evaluation. (United States)

    Lolekha, P H; Vanavanan, S; Lolekha, S


    Anion gap (AG) is a calculated value commonly used in clinical practice. It approximates the difference between the concentration of unmeasured anions (UA) and unmeasured cations (UC) in serum. At present, the reference range of anion gap has been lowered from 8-16 to 3-11 mmol/l because of the changes in technique for measuring electrolyte. However, clinicians and textbooks still refer and use the old reference value of 8-16 mmol/l. This may lead to misinterpretation of the value of anion gap. Our study updated the value of anion gap in clinical diagnosis and laboratory evaluation. Criteria for using anion gap were also suggested. We analyzed serum electrolyte using the Beckman Synchron CX5. The anion gap was calculated from the formula: [Na(+)-(Cl(-)+HCO(3)(-))]. We estimated the reference range using the non-parametric percentile estimation method. The reference range of anion gap obtained from 124 healthy volunteers was 5-12 mmol/l, which was low and confirmed the reports from other studies (3-11 mmol/l) using ion-selective electrode. From the retrospective study on the 6868 sets of serum electrolyte among hospitalized patients, we found the incidences of normal, increased, and decreased anion gaps were 59.5%, 37.6%, and 2.9%, respectively. The mean and central 90% range of increased anion gap were 16 and 13-20 mmol/l, which was lower than those reported in previous study (25 and 19-28 mmol/l). Anion gap exceeding 24 mmol/l was rare. The mean and central 90% range of decreased anion gap were 3 and 2-4 mmol/l, which were lower than those reported in previous study (6 and 3-8 mmol/l). The value of less than 2 mmol/l was rare. The most common causes of increased anion gap (hypertensive disease, chronic renal failure, malignant neoplasms, diabetes mellitus and heart diseases) and decreased anion gap (liver cirrhosis and nephrotic syndrome) in this study were similar to those in previous studies. We found two cases of IgG multiple myeloma with anion gap of 2 mmol

  13. Cholesterol and phytosterols differentially regulate the expression of caveolin 1 and a downstream prostate cell growth-suppressor gene (United States)

    Ifere, Godwin O.; Equan, Anita; Gordon, Kereen; Nagappan, Peri; Igietseme, Joseph U.; Ananaba, Godwin A.


    Background The purpose of our study was to show the distinction between the apoptotic and anti-proliferative signaling of phytosterols and cholesterol enrichment in prostate cancer cell lines, mediated by the differential transcription of caveolin-1, and N-myc downstream regulated gene1 (NDRG1), a pro-apoptotic androgen-regulated tumor suppressor. Methods PC-3 and DU145 cells were treated with sterols (cholesterol and phytosterols) for 72 h, followed by trypan blue dye exclusion measurement of necrosis and cell growth measured with a Coulter counter. Sterol induction of cell growth-suppressor gene expression was evaluated by mRNA transcription using RT-PCR, while cell cycle analysis was performed by FACS analysis. Altered expression of Ndrg1 protein was confirmed by Western blot analysis. Apoptosis was evaluated by real time RT-PCR amplification of P53, Bcl-2 gene and its related pro- and anti-apoptotic family members. Results Physiological doses (16 µM) of cholesterol and phytosterols were not cytotoxic in these cells. Cholesterol enrichment promoted cell growth (Pphytosterols significantly induced growth-suppression (Pphytosterols decreased mitotic subpopulations. We demonstrated for the first time that cholesterols concertedly attenuated the expression of caveolin-1(cav-1) and NDRG1 genes in both prostate cancer cell lines. Phytosterols had the opposite effect by inducing overexpression of cav-1, a known mediator of androgen-dependent signals that presumably control cell growth or apoptosis. Conclusions Cholesterol and phytosterol treatment differentially regulated the growth of prostate cancer cells and the expression of p53 and cav-1, a gene that regulates androgen-regulated signals. These sterols also differentially regulated cell cycle arrest, downstream pro-apoptotic androgen-regulated tumor-suppressor, NDRG1 suggesting that cav-1 may mediate pro-apoptotic NDRG1 signals. Elucidation of the mechanism for sterol modulation of growth and apoptosis signaling

  14. Desempenho da semeadora-adubadora MAGNUM 2850 em plantio direto no basalto paranaense Performance of MAGNUM 2850 seeder in nontillage in the basaltic soil of Paraná, Brazil

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    Full Text Available O sistema de plantio direto atingiu, no Estado do Paraná, cerca de dois milhões de hectares, nos últimos anos, e um dos seus principais entraves é a falta de semeadoras-adubadoras apropriadas para operação em solos argilosos e de origem basáltica. Portanto, estudou-se a MAGNUM 2850 PD quanto à demanda energética e desempenho operacional, com a finalidade de oferecer subsídios ao seu aperfeiçoamento, de acordo com a realidade paranaense. Observou-se que o esforço de tração variou em função da umidade e características mecânicas do solo, atingiu valores elevados, e exigiu, em situações extremas, potência de até 79,5 kW do trator. Os componentes de ataque ao solo mostraram um bom desempenho no corte da vegetação e na uniformidade da profundidade de trabalho, porém apresentaram grande área mobilizada no sulco. Obteve-se boa qualidade de semeadura, avaliada pela porcentagem e velocidade de emergência das sementes no campo. Foram identificados erros de dosagem do fertilizante e deposição das sementes, e principalmente na velocidade de deslocamento de 8,0 km/h; porém, foi boa a uniformidade transversal das sementes e plantas no campo.The nontillage system has attained two million hectars in the State of Paraná, Brazil, in the last years, but one of the most important problems was the lack of suitable seeders to basaltic clay soils. Therefore, MAGNUM 2850 PD was studied as for energetic requirement and operational performance in order to fit it to the reality of the State of Paraná. It was observed that the traction power varied in function of moisture and of mechanical characteristics of the soil, and attained high values and high power demand in maximal situations -- around 79.5 kW of the tractor. The soil coulter openers showed a good performance in cutting the vegetal and in depth uniformity of work; but they also showed a big mobilized area in the furrow. Good sowing quality was obtained, which was evaluated by the

  15. Determination of reference ranges for full blood count parameters in neonatal cord plasma in Hilla, Babil, Iraq

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    Al-Marzoki JM


    Full Text Available Jasim M Al-Marzoki1, Zainab W Al-Maaroof2, Ali H Kadhum31Department of Pediatrics, 2Department of Pathology, Babylon Medical College, 3Babylon Gynecology and Pediatric Teaching Hospital, Hilla, IraqBackground: The health of an individual is known to vary in different countries, in the same country at different times, and in the same individuals at different ages. This means that the condition of individuals must be related to or compared with reference data. Determination of a reference range for the healthy term newborn is clinically important in terms of various complete blood count parameters. The purpose of this study was to establish a local reference range for full blood count parameters in neonatal cord plasma in Hilla, Babil, Iraq.Methods: A total of 220 mothers and their neonates were enrolled in this cross-sectional study from February 2011 to January 2012. Maternal inclusion criteria were age 15–45 years, an uneventful pregnancy, and hemoglobin ≥ 10 g. Neonatal inclusion criteria were full term (37–42 weeks and normal birth weight. The umbilical cord was immediately clamped after delivery of the baby; 3 mL of cord blood was then taken from the umbilical vein and collected in a tube containing ethylenediamine tetra-acetic acid, its plasma was analyzed for full blood count parameters by standard Coulter gram, and the differential leukocyte count was done manually.Results: Mean neonatal hemoglobin was 13.88 ± 1.34 (range 11–17.3 g/dL and mean white cell count was 10.12 ± 2.8 (range 3.1–21.6 × 109/L. Mean platelet count was 267.63 ± 60.62 (range 152–472 × 109/L. No significant differences in red cell, white cell, or platelet counts were found between males and females, except for neutrophil count. The current study shows lower levels of hemoglobin, white cells, and red cells compared with other studies, and there is agreement with some studies and disagreement with others concerning platelet count.Conclusion: Most results

  16. A method to increase reproducibility in adult ventricular myocyte sizing and flow cytometry: Avoiding cell size bias in single cell preparations.

    Directory of Open Access Journals (Sweden)

    Javier E López

    Full Text Available Flow cytometry (FCM of ventricular myocytes (VMs is an emerging technology in adult cardiac research that is challenged by the wide variety of VM shapes and sizes. Cellular variability and cytometer flow cell size can affect cytometer performance. These two factors of variance limit assay validity and reproducibility across laboratories. Washing and filtering of ventricular cells in suspension are routinely done to prevent cell clumping and minimize data variability without the appropriate standardization. We hypothesize that washing and filtering arbitrarily biases towards sampling smaller VMs than what actually exist in the adult heart.To determine the impact of washing and filtering on adult ventricular cells for cell sizing and FCM.Left ventricular cardiac cells in single-cell suspension were harvested from New Zealand White rabbits and fixed prior to analysis. Each ventricular sample was aliquoted before washing or filtering through a 40, 70, 100 or 200μm mesh. The outcomes of the study are VM volume by Coulter Multisizer and light-scatter signatures by FCM. Data are presented as mean±SD. Myocyte volumes without washing or filtering (NF served as the "gold standard" within the sample and ranged from 11,017 to 46,926μm3. Filtering each animal sample through a 200μm mesh caused no variation in the post-filtration volume (1.01+0.01 fold vs. NF, n = 4 rabbits, p = 0.999 with an intra-assay coefficient of variation (%CV of <5% for all 4 samples. Filtering each sample through a 40, 70 or 100μm mesh invariably reduced the post-filtration volume by 41±10%, 9.0±0.8% and 8.8±0.8% respectively (n = 4 rabbits, p<0.0001, and increased the %CV (18% to 1.3%. The high light-scatter signature by FCM, a simple parameter for the identification of ventricular myocytes, was measured after washing and filtering. Washing discarded VMs and filtering cells through a 40 or 100μm mesh reduced larger VM by 46% or 11% respectively (n = 6 from 2 rabbits, p<0

  17. Interstitial diffuse radiance spectroscopy of gold nanocages and nanorods in bulk muscle tissues

    Directory of Open Access Journals (Sweden)

    Grabtchak S


    Full Text Available Serge Grabtchak,1,2 Logan G Montgomery,1 Bo Pang,3,4 Yi Wang,4,5 Chao Zhang,6,7 Zhiyuan Li,6,7 Younan Xia,4,8 William M Whelan1,91Department of Physics, University of Prince Edward Island, Charlottetown, PEI, Canada; 2Departments of Electrical and Computer Engineering, and Physics, Dalhousie University, Halifax, Canada; 3Department of Biomedical Engineering, Peking University, Beijing, People’s Republic of China; 4The Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA; 5Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing, People’s Republic of China; 6Laboratory of Optical Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, People’s Republic of China; 7College of Physics and Optoelectronics, South China University of Technology, Guangzhou, People’s Republic of China; 8School of Chemistry and Biochemistry, and School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, USA; 9Atlantic Veterinary College, Charlottetown, PEI, CanadaAbstract: Radiance spectroscopy was applied to the interstitial detection of localized inclusions containing Au nanocages or nanorods with various concentrations embedded in porcine muscle phantoms. The radiance was quantified using a perturbation approach, which enabled the separation of contributions from the porcine phantom and the localized inclusion, with the inclusion serving as a perturbation probe of photon distributions in the turbid medium. Positioning the inclusion at various places in the phantom allowed for tracking of photons that originated from a light source, passed through the inclusion’s location, and reached a detector. The inclusions with high extinction coefficients were able to absorb nearly all photons in the range of 650–900 nm, leading to a spectrally flat radiance signal. This signal could be

  18. Albedo Spatial Variability and Causes on the Western Greenland Ice Sheet Percolation Zone (United States)

    Lewis, G.; Osterberg, E. C.; Hawley, R. L.; Koffman, B. G.; Marshall, H. P.; Birkel, S. D.; Dibb, J. E.


    Many recent studies have concluded that Greenland Ice Sheet (GIS) mass loss has been accelerating over recent decades, but spatial and temporal variations in GIS mass balance remain poorly understood due to a complex relationship among precipitation and temperature changes, increasing melt and runoff, ice discharge, and surface albedo. Satellite measurements from MODerate resolution Imaging Spectroradiometer (MODIS) indicate that albedo has been declining over the past decade, but the cause and extent of GIS albedo change remains poorly constrained by field data. As fresh snow (albedo > 0.85) warms and melts, its albedo decreases due to snow grain growth, promoting solar absorption, higher snowpack temperatures and further melt. However, dark impurities like soot and dust can also significantly reduce snow albedo, even in the dry snow zone. While many regional climate models (e.g. the Regional Atmospheric Climate MOdel - RACMO2) calculate albedo spatial resolutions on the order of 10-30 km, and MODIS averages albedo over 500 m, surface features like sastrugi can affect albedo on much smaller scales. Here we assess the relative importance of grain size and shape vs. impurity concentrations on albedo in the western GIS percolation zone. We collected broadband albedo measurements (300-2500 nm at 3-8 nm resolution) at 35 locations using an ASD FieldSpec4 spectroradiometer to simultaneously quantify radiative fluxes and spectral reflectance. Measurements were collected on 10 x 10 m, 1 x 1 km, 5 x 5 km, and 10 x 10 km grids to determine the spatial variability of albedo as part of the 850-km Greenland Traverse for Accumulation and Climate Studies (GreenTrACS) traverse from Raven/Dye 2 to Summit. Additionally, we collected shallow (0-50 cm) snow pit samples every 5 cm at ASD measurement sites to quantify black carbon and mineral dust concentrations and size distributions using a Single Particle Soot Photometer and Coulter Counter, respectively. Preliminary results

  19. Comparison of manual and automated cell counts in EDTA preserved synovial fluids. Storage has little influence on the results. (United States)

    Salinas, M; Rosas, J; Iborra, J; Manero, H; Pascual, E


    To determine the precision and agreement of synovial fluid (SF) cell counts done manually and with automated counters, and to determine the degree of variability of the counts in SF samples, kept in the tubes used for routine white blood cell (WBC) counts--which use liquid EDTA as anticoagulant--at 24 and 48 hours at 4 degrees C, and at room temperature. To determine precision, cell counts were repeated 10 times--both manually and by an automated counter--in a SF sample of low, medium, and high cellularity. The variances were calculated to determine the interobserver variation in two manual (M1,M2) and two automated cell counts (C1,C2). The agreement between a manual (M1) and automated counter (C1) results, was analysed by the Bland and Altman method and the difference against the mean of the two methods was plotted. Then, the mean difference between the two methods was estimated and the standard deviation of the difference. To determine the effects of storage, SF samples were kept in a refrigerator at 4 degrees C, and at room temperature; cell counts were done manually (M1) and automatically (C1) at 24 and 48 hours and the changes analysed by the Bland and Altman method. The variances were compared using an F test. (1) Precision. With the manual technique, the coefficients of variation were 27.9%, 14%, and 10.7% when used for counting the SF with low (270), medium (6200), and high cellularities (25,000). With the automated technique the coefficients of variation were 20%, 3.4%, and 2.9% in the same SF samples. In the fluids of medium and high cellularity, the variances of the automated cell counts were significatively lower (F test, p automated counter. (4) Influence of storage. The coulter counts of SF samples preserved at 4 degrees C showed less variance (F test, p Automated cell count of the SF offers advantages: it gives higher precision and consumes less time. The stability of the samples preserved in the EDTA tubes used for routine WBC counts is of

  20. Comparison of Series of Vugs and Non-vuggy Synthetic Porous Media on Formation Damage (United States)

    Khan, H.; DiCarlo, D. A.; Prodanovic, M.


    Produced water reinjection (PWRI) is an established cost-effective oil field practice where produced water is injected without any cleanup, for water flooding or disposal. Resultantly the cost of fresh injection fluid and/or processing produced water is saved. A common problem with injection of unprocessed water is formation damage in the near injection zone due to solids (fines) entrapment, causing a reduction in permeability and porosity of the reservoir. Most studies have used homogeneous porous media with unimodal grain sizes, while real world porous media often has a wide range of pores, up to and including vugs in carbonaceous rocks. Here we fabricate a series of vugs in synthetic porous media by sintering glass beads with large dissolvable inclusions. The process is found to be repeatable, allowing a similar vug configuration to be tested for different flow conditions. Bi-modal glass bead particles (25 & 100 micron) are injected at two different flow rates and three different injection concentrations. Porosity, permeability and effluent concentration are determined using CT scanning, pressure measurements and particle counting (Coulter counter), respectively. Image analysis is performed on the CT images to determine the change in vug size for each flow condition. We find that for the same flow conditions, heterogeneous media with series of vugs have an equal or greater permeability loss compared to homogeneous porous media. A significant change in permeability is observed at the highest concentration and flow rate as more particles approach the filter quickly, resulting in a greater loss in permeability in the lower end of the core. Image analysis shows the highest loss in vug size occurs at the low flow rate and highest concentration. The lower vug is completely blocked for this flow case. For all flow cases lower values of porosity are observed after the core floods. At low flow rate and medium concentration, a drastic loss in porosity is observed in the

  1. Science education through open and distance learning at Higher Education level

    Directory of Open Access Journals (Sweden)

    Amrita NIGAM


    Full Text Available Abstract The changes faced by the society in the past few decades brought revolution in all areas. The job requirements have undergone change tremendously. The emergence of e-culture, e-education, e-governance, e-training, e-work sites and so on questioned the capacity of conventional face to face education in catering to all and relevance of existing job related skills to a great extent in the emerging global society. Today, every one has to update his/her educational and/or professional skills and competencies to cope up with the emerging work challenges. This is more so in the field of science and technology. At the same time, it is impossible to cater to educational and training opportunities to one and all those who aspire for it through the conventional set up. The distance and open learning (ODL seems to be one of the viable alternatives. Today, the success and viability of ODL is accepted globally. Coulter (1989, through a study demonstrated that ODL is a cost-effective medium in providing educational opportunities. Similarly Holmberg (1981 also mentioned ODL as a systematic teaching-learning medium by using variety of medium for imparting learning. The present study is an attempt to study the experiences of the open science learners of IGNOU on different aspect of the science higher education. Here a questionnaire was used to collect the data and responses from 81 students enrolled for B. Sc. from IGNOU were collected. The findings of the study reported that society has undergone drastic changes in the last few decades. The revolution led due to Information and Communication Technologies (ICTs have widely affected all aspects of society. The emerging jobs require entirely new skills and competencies i.e., employment in BPOs or switching over to e-governance, e-Banking and e- based sectors. Even e-learning has made numerous expectations from teachers and other personnel. The use of ICTs in almost every field needs adequately trained

  2. Proliferative vitreo-retinal disorders: experimental models in vivo and in vitro. (United States)

    Martini, B


    The aim of the present thesis was to develop, refine, and assess experimental models for the study of proliferative vitreo-retinal disorders. An intravitreal injection of a colloidal solution of microparticles was used in the primate eye to produce pathologic changes including intraocular cell invasion, cell proliferation, neovascularization, collagen synthesis, and tractional retinal detachment. In a separate primate model for laser-induced subretinal neovascularization, the origin and the occurrence of macrophages was evaluated. Examinations were performed using ophthalmoscopy, slit-lamp microscopy, light microscopy, and transmission electron microscopy. Cell cultures were employed to study the effects of vitreous humor and macrophages on the proliferation of cultured retinal pigment epithelial (RPE) cells and cultured fibroblasts using a Coulter counter. Morphologic changes were documented by phase micrography. A quantitative estimation of the extracellular matrix deposition of fibrous proteins by macrophage-modulated RPE cells as well as by vitreous-modulated RPE cells was done using enzymatic digestion and radioactive labeling techniques. A qualitative analysis of the types of collagen that was deposited in the extracellular matrices by vitreous modulated cultures was also made using indirect immunofluorescence. Using a newly developed RPE cell specific monoclonal antibody, the avidin-biotin-peroxidase labeling technique was finally employed to test the phenotypic epitope expression of macrophage-modulated and non-modulated RPE cells. A new experimental in vivo model for pathologic changes that characterize proliferative vitreo-retinal disorders was developed in the primate eye. In the model for laser-induced subretinal neovascularization, macrophages were shown to be principally recruited from the systemic circulation. Using cell cultures, it was found that both macrophage-conditioned medium and vitreous humor, separately or combined, exert mitogenic effects

  3. An epigenome-wide association study of total serum IgE in Hispanic children. (United States)

    Chen, Wei; Wang, Ting; Pino-Yanes, Maria; Forno, Erick; Liang, Liming; Yan, Qi; Hu, Donglei; Weeks, Daniel E; Baccarelli, Andrea; Acosta-Perez, Edna; Eng, Celeste; Han, Yueh-Ying; Boutaoui, Nadia; Laprise, Catherine; Davies, Gwyneth A; Hopkin, Julian M; Moffatt, Miriam F; Cookson, William O C M; Canino, Glorisa; Burchard, Esteban G; Celedón, Juan C


    Total IgE is a therapeutic target in patients with allergic diseases. DNA methylation in white blood cells (WBCs) was associated with total IgE levels in an epigenome-wide association study of white subjects. Whether DNA methylation of eosinophils explains these findings is insufficiently understood. We tested for association between genome-wide DNA methylation in WBCs and total IgE levels in 2 studies of Hispanic children: the Puerto Rico Genetics of Asthma and Lifestyle Study (PR-GOAL; n = 306) and the Genes-environments and Admixture in Latino Americans (GALA II) study (n = 573). Whole-genome methylation of DNA from WBCs was measured by using the Illumina Infinium HumanMethylation450 BeadChip. Total IgE levels were measured by using the UniCAP 100 system. In PR-GOAL WBC types (ie, neutrophils, eosinophils, basophils, lymphocytes, and monocytes) in peripheral blood were measured by using Coulter Counter techniques. In the GALA II study WBC types were imputed. Multivariable linear regression was used for the analysis of DNA methylation and total IgE levels, which was first conducted separately for each cohort, and then results from the 2 cohorts were combined in a meta-analysis. CpG sites in multiple genes, including novel findings and results previously reported in white subjects, were significantly associated with total IgE levels. However, adjustment for WBC types resulted in markedly fewer significant sites. Top findings from this adjusted meta-analysis were in the genes ZFPM1 (P = 1.5 × 10 -12 ), ACOT7 (P = 2.5 × 10 -11 ), and MND1 (P = 1.4 × 10 -9 ). In an epigenome-wide association study adjusted for WBC types (including eosinophils), methylation changes in genes enriched in pathways relevant to asthma and immune responses were associated with total IgE levels among Hispanic children. Copyright © 2017 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  4. A visual basic program to generate sediment grain-size statistics and to extrapolate particle distributions (United States)

    Poppe, L.J.; Eliason, A.H.; Hastings, M.E.


    Measures that describe and summarize sediment grain-size distributions are important to geologists because of the large amount of information contained in textural data sets. Statistical methods are usually employed to simplify the necessary comparisons among samples and quantify the observed differences. The two statistical methods most commonly used by sedimentologists to describe particle distributions are mathematical moments (Krumbein and Pettijohn, 1938) and inclusive graphics (Folk, 1974). The choice of which of these statistical measures to use is typically governed by the amount of data available (Royse, 1970). If the entire distribution is known, the method of moments may be used; if the next to last accumulated percent is greater than 95, inclusive graphics statistics can be generated. Unfortunately, earlier programs designed to describe sediment grain-size distributions statistically do not run in a Windows environment, do not allow extrapolation of the distribution's tails, or do not generate both moment and graphic statistics (Kane and Hubert, 1963; Collias et al., 1963; Schlee and Webster, 1967; Poppe et al., 2000)1.Owing to analytical limitations, electro-resistance multichannel particle-size analyzers, such as Coulter Counters, commonly truncate the tails of the fine-fraction part of grain-size distributions. These devices do not detect fine clay in the 0.6–0.1 μm range (part of the 11-phi and all of the 12-phi and 13-phi fractions). Although size analyses performed down to 0.6 μm microns are adequate for most freshwater and near shore marine sediments, samples from many deeper water marine environments (e.g. rise and abyssal plain) may contain significant material in the fine clay fraction, and these analyses benefit from extrapolation.The program (GSSTAT) described herein generates statistics to characterize sediment grain-size distributions and can extrapolate the fine-grained end of the particle distribution. It is written in Microsoft

  5. Characteristics of mineral aerosol deposited on the glaciers of Mt. Elbrus, Caucasus, Russia. (United States)

    Kutuzov, Stanislav; Shahgedanova, Maria; Mikhalenko, Vladimir; Ginot, Patrick; Lavrentiev, Ivan; Popov, Gregory


    Records of mineral aerosol (desert dust) stored in glaciers provide data on frequency and intensity of deposition events, source regions and atmospheric pathways of mineral dust. We present and discuss a chronology of dust deposition events recorded in the shallow firn and ice cores extracted on the Western Plateau, Mt. Elbrus (5150 m a.s.l.), Caucasus Mountains, Russia and covering the period of 2009-2013. Particle size distribution and chemical analysis (major ions, trace elements) were peformed using Coulter Counter Multisizer III, Abacus particle counter, IC and ICPMS analysis. Sampling was performed using continuous flow analysis (CFA) system. Annual average dust flux (264 μg/cm2 a-1) and average mass concentration (1.7 mg/kg) over the period 2007-2013 were calculated for the first time for this region. A combination of satellite imagery (MSG SEVIRI), trajectory models (FLEXTA, HYSPLIT) and meteorological data were used to accurately date each of the dust layers observed in shallow cores and investigate provenance of the dust and its pathways. Desert dust originating from the Middle East and Sahara was deposited on the Caucasus glaciers 3-6 times a year. Although less frequent, Saharan events are characterized by considerably higher dust loads than the more frequent Middle Eastern events. The mass median diameter of dust particles ranged between 2 and 9 μm. The deposition of dust resulted in elevated concentrations of most ions, especially Ca2+, Mg2+, K+, and sulphates. Dust originated from or passing over the Middle East was characterised by the elevated concentrations of nitrates and ammonia. This may be related to dust emissions from agricultural fields which, if abandoned due to droughts, become important sources of dust. By contrast, samples of the Saharan dust originated from natural sources showed lower concentrations of ammonium. The mean values of crustal enrichment factors for the measured trace elements including metals were calculated. Overall

  6. Report on the Second ARM Mobile Facility (AMF2) Roll, Pitch, and Heave (RPH) Stabilization Platform: Design and Evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Coulter, Richard L. [Argonne National Lab. (ANL), Argonne, IL (United States); Martin, Timothy J. [Argonne National Lab. (ANL), Argonne, IL (United States)


    One of the primary objectives of the U.S. Department of Energy’s Atmospheric Radiation Measurement (ARM) Climate Research Facility’s second Mobile Facility (AMF2) is to obtain reliable measurements of solar, surface, and atmospheric radiation, as well as cloud and atmospheric properties, from ocean-going vessels. To ensure that these climatic measurements are representative and accurate, many AMF2 instrument systems are designed to collect data in a zenith orientation. A pillar of the AMF2 strategy in this effort is the use of a stable platform. The purpose of the platform is to 1) mitigate vessel motion for instruments that require a truly vertical orientation and keep them pointed in the zenith direction, and 2) allow for accurate positioning for viewing or shading of the sensors from direct sunlight. Numerous ARM instruments fall into these categories, but perhaps the most important are the vertically pointing cloud radars, for which vertical motions are a critical parameter. During the design and construction phase of AMF2, an inexpensive stable platform was purchased to perform the stabilization tasks for some of these instruments. The first table compensated for roll, pitch, and yaw (RPY) and was reported upon in a previous technical report (Kafle and Coulter, 2012). Subsequently, a second table was purchased specifically for operation with the Marine W-band cloud radar (MWACR). Computer programs originally developed for RPY were modified to communicate with the new platform controller and with an inertial measurements platform that measures true ship motion components (roll, pitch, yaw, surge, sway, and heave). This platform could not be tested dynamically for RPY because of time constraints requiring its deployment aboard the container ship Horizon Spirit in September 2013. Hence the initial motion tests were conducted on the initial cruise. Subsequent cruises provided additional test results. The platform, as tested, meets all the design and

  7. Proceedings of Patient Reported Outcome Measure’s (PROMs Conference Sheffield 2016: advances in patient reported outcomes research

    Directory of Open Access Journals (Sweden)

    Tim Croudace


    longitudinal parameter shifts in epidemiological data: measurement invariance and response shifts in cohort and survey data describing the UK’s Quality of Life Jan R. Boehnke O12 Patient-reported outcomes within health technology decision making: current status and implications for future policy Andrew Trigg, Ruth Howells O13 Can social care needs and well-being be explained by the EQ-5D? Analysis of Health Survey for England dataset Jeshika Singh, Subhash Pokhrel, Louise Longworth O14 Where patients and policy meet: exploring individual-level use of the Long-Term Conditions Questionnaire (LTCQ Caroline Potter, Cheryl Hunter, Laura Kelly, Elizabeth Gibbons, Julian Forder, Angela Coulter, Ray Fitzpatrick, Michele Peters

  8. Determination of reference ranges for full blood count parameters in neonatal cord plasma in Hilla, Babil, Iraq (United States)

    Al-Marzoki, Jasim M; Al-Maaroof, Zainab W; Kadhum, Ali H


    Background The health of an individual is known to vary in different countries, in the same country at different times, and in the same individuals at different ages. This means that the condition of individuals must be related to or compared with reference data. Determination of a reference range for the healthy term newborn is clinically important in terms of various complete blood count parameters. The purpose of this study was to establish a local reference range for full blood count parameters in neonatal cord plasma in Hilla, Babil, Iraq. Methods A total of 220 mothers and their neonates were enrolled in this cross-sectional study from February 2011 to January 2012. Maternal inclusion criteria were age 15–45 years, an uneventful pregnancy, and hemoglobin ≥ 10 g. Neonatal inclusion criteria were full term (37–42 weeks) and normal birth weight. The umbilical cord was immediately clamped after delivery of the baby; 3 mL of cord blood was then taken from the umbilical vein and collected in a tube containing ethylenediamine tetra-acetic acid, its plasma was analyzed for full blood count parameters by standard Coulter gram, and the differential leukocyte count was done manually. Results Mean neonatal hemoglobin was 13.88 ± 1.34 (range 11–17.3) g/dL and mean white cell count was 10.12 ± 2.8 (range 3.1–21.6) × 109/L. Mean platelet count was 267.63 ± 60.62 (range 152–472) × 109/L. No significant differences in red cell, white cell, or platelet counts were found between males and females, except for neutrophil count. The current study shows lower levels of hemoglobin, white cells, and red cells compared with other studies, and there is agreement with some studies and disagreement with others concerning platelet count. Conclusion Most results in the current study were within the reference range. The hematological reference values for Iraqi neonatal cord plasma need to be confirmed by larger numbers of blood samples and by collecting samples from

  9. Characteristics of Dust Deposition at High Elevation Sites in Caucasus Over the Past 190 years Recorded in Ice Cores. (United States)

    Kutuzov, Stanislav; Ginot, Patrick; Mikhaenko, Vladimir; Krupskaya, Victoria; Legrand, Michel; Preunkert, Suzanne; Polukhov, Alexey; Khairedinova, Alexandra


    The nature and extent of both radiative and geochemical impacts of mineral dust on snow pack and glaciers depend on physical and chemical properties of dust particles and its deposition rates. Ice cores can provide information about amount of dust particles in the atmosphere and its characteristic and also give insights on strengths of the dust sources and its changes in the past. A series of shallow ice cores have been obtained in Caucasus mountains, Russia in 2004 - 2015. A 182 meter ice core has been recovered at the Western Plateau of Mt. Elbrus (5115 m a.s.l.) in 2009. The ice cores have been dated using stable isotopes, NH4+ and succinic acid data with the seasonal resolution. Samples were analysed for chemistry, concentrations of dust and black carbon, and particle size distributions. Dust mineralogy was assessed by XRD. Individual dust particles were analysed using SEM. Dust particle number concentration was measured using the Markus Klotz GmbH (Abakus) implemented into the CFA system. Abakus data were calibrated with Coulter Counter multisizer 4. Back trajectory cluster analysis was used to assess main dust source areas. It was shown that Caucasus region experiencing influx of mineral dust from the Sahara and deserts of the Middle East. Mineralogy of dust particles of desert origin was significantly different from the local debris material and contained large proportion of calcite and clay minerals (kaolinite, illite, palygorskite) associated with material of desert origin. Annual dust flux in the Caucasus Mountains was estimated as 300 µg/cm2 a-1. Particle size distribution depends on individual characteristics of dust deposition event and also on the elevation of the drilling site. The contribution of desert dust deposition was estimated as 35-40 % of the total dust flux. Average annual Ca2+ concentration over the period from 1824 to 2013 was of 150 ppb while some of the strong dust deposition events led to the Ca2+ concentrations reaching 4400 ppb. An

  10. Sedimentology, geochemistry and rock magnetic properties of beach sands in Galapagos Islands - implications for nesting marine turtles (United States)

    Perez-Cruz, L.; Urrutia-Fucugauchi, J.; Vazquez-Gutierrez, F.; Carranza-Edwards, A.


    Marine turtles are well known for their navigation ability in the open ocean and fidelity to nesting beaches. Green turtle adult females migrate from foraging areas to island nesting beaches, traveling hundreds or thousands of kilometers each way. The marine turtle breeding in the Galapagos Islands is the Green Sea Turtle (Chelonia mydas agassisi); fairly common throughout the islands but with nesting sites located at Las Bachas (Santa Cruz), Barahona and Quinta Playa (Isabela), Salinas (Baltra), Gardner Bay (Española) and Bartolomé Islet. In order to characterize and to identify the geochemical signature of nesting marine turtle beaches in Galapagos Islands, sedimentological, geochemical and rock magnetic parameters are used. A total of one hundred and twenty sand samples were collected in four beaches to relate compositional characteristics between equivalent areas, these are: Las Bachas, Salinas, Barahona and Quinta Playa. Grain size is evaluated using laser particle analysis (Model Coulter LS 230). Bulk ICP-MS geochemical analysis is performed, following trace elements are analyzed: Al, V, Cr, Co, Ni, Cu, Zn, Cd, Ba, Pb, Fe, Mn, K, Na, Mg, Sr, Ca and Hg; and low-field magnetic susceptibility is measured in all samples at low and high frequencies. Granulometric analysis showed that Barahona and Quinta Playa are characterized for fine grained sands. In contrast, Salinas and Las Bachas exhibit medium to coarse sands. Trace metals concentrations and magnetic susceptibility show different distribution patterns in the beach sands. Calcium is the most abundant element in the samples. In particular, Co, K, and Na show similar concentrations in the four beaches. Las Bachas beach shows highest concentrations of Pb and Hg (maximum values 101.1 and 118.5 mg/kg, respectively), we suggest that the enrichment corresponds to an anthropogenic signal. Salinas beach samples show high concentrations of Fe, V, Cr, Zn, Mn and the highest values of magnetic susceptibility (maximum

  11. Effects of CO2 on particle size distribution and phytoplankton abundance during a mesocosm bloom experiment (PeECE II

    Directory of Open Access Journals (Sweden)

    M. Schartau


    Full Text Available The influence of seawater carbon dioxide (CO2 concentration on the size distribution of suspended particles (2–60 μm and on phytoplankton abundance was investigated during a mesocosm experiment at the large scale facility (LFS in Bergen, Norway, in the frame of the Pelagic Ecosystem CO2 Enrichment study (PeECE II. In nine outdoor enclosures the partial pressure of CO2 in seawater was modified by an aeration system to simulate past (~190 μatm CO2, present (~370 μatm CO2 and future (~700 μatm CO2 CO2 conditions in triplicates. Due to the initial addition of inorganic nutrients, phytoplankton blooms developed in all mesocosms and were monitored over a period of 19 days. Seawater samples were collected daily for analysing the abundance of suspended particles and phytoplankton with the Coulter Counter and with Flow Cytometry, respectively. During the bloom period, the abundance of small particles (2 levels. At that time, a direct relationship between the total-surface-to-total-volume ratio of suspended particles and DIC concentration was determined for all mesocosms. Significant changes with respect to the CO2 treatment were also observed in the phytoplankton community structure. While some populations such as diatoms seemed to be insensitive to the CO2 treatment, others like Micromonas spp. increased with CO2, or showed maximum abundance at present day CO2 (i.e. Emiliania huxleyi. The strongest response to CO2 was observed in the abundance of small autotrophic nano-plankton that strongly increased during the bloom in the past CO2 mesocosms. Together, changes in particle size distribution and phytoplankton community indicate a complex interplay between the ability of the cells to physiologically respond to changes in CO2 and size selection. Size of cells is of general importance for a variety of processes in marine systems such as diffusion-limited uptake of substrates, resource allocation, predator-prey interaction, and gravitational settling

  12. Cytometric evaluation of intracellular IFN-γ and IL-4 levels in thyroid follicular cells from patients with autoimmune thyroid diseases

    Directory of Open Access Journals (Sweden)

    Bossowski Artur


    Full Text Available Abstract Background In recent few years is underlined that altered balance of pro- and anti-inflammatory cytokines play an important role in the pathogenesis of AITD. The aim of this study was to estimate intracellular INF-γ and IL-4 levels in thyroid-infiltrating lymphocytes and thyrocytes isolated from thyroid tissues in 54 adolescent patients aged 8-21 years, with Graves' disease (GD; n = 18, Hashimoto's thyroiditis (HT; n = 18 and non-toxic multinodular goiter (NTMG; n = 18. Methods Fresh thyroid tissues were taken on culture medium RPMI -1640, it was mechanically prepared. In next step were added cell activators -12- myristate 13- the acetate (PMA and Ionomycin as well as the inhibitor of transportation of proteins - Breferdin A. They were cultured 24 hours in 50 ml flasks at 37°C in a 5-95% CO2-air water-saturated atmosphere. After that, thyrocytes were identified by mouse mAb directed against human TPO epitope 64 conjugated with rabbit anti-mouse antibodies IgG (Fab'2 labeled by FITC. After incubation at room temperature to each of samples added reagent A fixative the cellular membrane. In next step into the cell suspensions were added reagent B to permeabilization of cellular membrane and specific anti-IL-4-PE or anti-IFN-γ-PE mAbs. Identification of intracellular cytokines in T lymphocytes was performed in the same procedure with application of anti-CD4-PerCP and anti-CD8-PerCP mAbs specific for T lymphocytes. The cells were analyzed in a flow cytometry (Coulter EPICS XL. Results In examined group of patients with GD we observed statistically significant higher mean percentage of cells with phenotype CD4+IL-4 (p Conclusions We conclude that human thyrocytes in autoimmune thyroid disorders could be a source of cytokine production and that their activation influences local interaction with T lymphocytes inflowing to the thyroid gland.

  13. 50 years LASERS: in vitro diagnostics, clinical applications and perspectives. (United States)

    Spyropoulos, Basile


    1960 Theodore Maiman built the first Ruby-LASER, starting-point for half a century of R&D on Biomedical LASER continuous improvement. The purpose of this paper is to contribute a review of the often disregarded, however, extremely important Industrial Property documents of LASER-based in vitro Diagnostics devices. It is an attempt to sketch-out the patent-trail leading towards the modern Biomedical Laboratory and to offer an introduction to the employment of "exotic" systems, such as the Free Electron LASER (FEL), that are expected to focus on the fundamental processes of life, following chemical reactions and biological processes as they happen, on unprecedented time and size scales. There are various in vitro LASER applications, however, the most important ones include: Hybrid Coulter Principle-LASER Hematology Analyzers. Flow Cytometry systems. Fluorescent in situ Hybridization (FISH Techniques). Confocal LASER Scanning Microscopy and Cytometry. From the first fluorescence-based flow Cytometry device developed in 1968 by Wolfgang Göhde until nowadays, numerous improvements and new features related to these devices appeared. The relevant industrial property milestone-documents and their overall numeral trends are presented. In 1971, J. Madey invented and developed the Free Electron LASER (FEL), a vacuum-tube that uses a beam of relativistic electrons passing through a periodic, transverse magnetic field (wiggler) to produce coherent radiation, contained in an optical cavity defined by mirrors. A resonance condition that involves the energy of the electron beam, the strength of the magnetic field, and the periodicity of the magnet determines the wavelength of the radiation. The FEL Coherent Light Sources like the Linac Coherent Light Source (LCLS) at Stanford, CA, USA or the Xray Free Electron LASER (XFEL) at Hamburg, Germany, will work much like a high-speed (LASERS. If the diodes brought a LASER into almost everyone's pocket, the above-mentioned super

  14. Growth inhibitory activity of extracts and compounds from Cimicifuga species on human breast cancer cells. (United States)

    Einbond, Linda Saxe; Wen-Cai, Ye; He, Kan; Wu, Hsan-au; Cruz, Erica; Roller, Marc; Kronenberg, Fredi


    The purpose of this report is to explore the growth inhibitory effect of extracts and compounds from black cohosh and related Cimicifuga species on human breast cancer cells and to determine the nature of the active components. Black cohosh fractions enriched for triterpene glycosides and purified components from black cohosh and related Asian species were tested for growth inhibition of the ER(-) Her2 overexpressing human breast cancer cell line MDA-MB-453. Growth inhibitory activity was assayed using the Coulter Counter, MTT and colony formation assays. Results suggested that the growth inhibitory activity of black cohosh extracts appears to be related to their triterpene glycoside composition. The most potent Cimicifuga component tested was 25-acetyl-7,8-didehydrocimigenol 3-O-beta-d-xylopyranoside, which has an acetyl group at position C-25. It had an IC(50) of 3.2microg/ml (5microM) compared to 7.2microg/ml (12.1microM) for the parent compound 7,8-didehydrocimigenol 3-O-beta-d-xylopyranoside. Thus, the acetyl group at position C-25 enhances growth inhibitory activity. The purified triterpene glycoside actein (beta-d-xylopyranoside), with an IC(50) equal to 5.7microg/ml (8.4microM), exhibited activity comparable to cimigenol 3-O-beta-d-xyloside. MCF7 (ER(+)Her2 low) cells transfected for Her2 are more sensitive than the parental MCF7 cells to the growth inhibitory effects of actein from black cohosh, indicating that Her2 plays a role in the action of actein. The effect of actein on Her2 overexpressing MDA-MB-453 and MCF7 (ER(+)Her2 low) human breast cancer cells was examined by fluorescent microscopy. Treatment with actein altered the distribution of actin filaments and induced apoptosis in these cells. These findings, coupled with our previous evidence that treatment with the triterpene glycoside actein induced a stress response and apoptosis in human breast cancer cells, suggest that compounds from Cimicifuga species may be useful in the prevention and

  15. Polymerization time for a microwave-cured acrylic resin with multiple flasks Tempo de polimerização de resina acrílica em microondas, utilizando múltiplas muflas

    Directory of Open Access Journals (Sweden)

    Daniela Maffei Botega


    Full Text Available This study aimed at establishing the polymerization time of a microwave-cured acrylic resin (AcronTM MC, simultaneously processing 2, 4, and 6 flasks. Required time was measured according to the parameters: monomer release in water, Knoop hardness, and porosity. Samples were made with AcronTM MC in different shapes: rectangular (32 x 10 x 2.5 mm for monomer release and porosity; and half-disc (30 mm in diameter x 4 mm in height for Knoop hardness. There were four experimental groups (n = 24 per group: G1 one flask (control; G2 two flasks; G3 four flasks, and G4 six flasks. At first, polymerization protocol was similar for all groups (3 min/450 W. Time was then adjusted for G2, G3, and G4, based on monomer release evaluation in the control group, obtained by spectrophotometer Beckman DU-70, with emitting wave of 206 nm. Knoop hardness test was performed using a Shimadzu HMV 2000 hardness tester, and 10 indentations were performed on each specimen's surface. Porosity was assessed after specimens were immersed in black ink and the pores counted in a microscope. Results showed that the complete polymerization of the resin occurred in 4.5 min for two flasks (G2; 8.5 min for four flasks (G3; and 13 min for six flasks (G4, all with 450 W. Statistical analysis revealed that the number of flasks does not interfere with polymerization, Knoop hardness, and porosity of the resin. Results showed that polymerization of microwave-curing resin with more than one flask is a viable procedure, as long as polymerization time is adjusted.O objetivo deste estudo foi o de determinar os tempos necessários para a polimerização padrão de uma resina acrílica em microondas, utilizando várias muflas simultaneamente. Os tempos necessários foram aferidos por parâmetros como monômeros liberados em água, dureza Knoop e porosidade. As amostras, confeccionadas em resina AcronTM MC, apresentavam as seguintes dimensões: para os parâmetros monômero residual e porosidade

  16. Atividade elétrica cerebral do rato com lesões da formação reticular mesencefálica Electrocorticographic study of the rats's bram after lesioning of the midbrain reticular formation

    Directory of Open Access Journals (Sweden)

    Walter C. Pereira


    Full Text Available No presente estudo foram utilizados 73 ratos em preparações agudas e crônicas, nas quais lesamos a formação reticular mesencefálica com corrente contínua (3,5 a 4,0 mA durante 5 a 10 segundos. O eletródio ativo era implantado estereotàxicamente segundo as coordenadas de König e Klippel. As lesões eram feitas parcial ou totalmente, uni ou bilateralmente, e em todos os animais procedeu-se ao controle histológico das áreas lesadas, usando-se o método de Weil. O registro da atividade elétrica cortical foi feito com polígrafo Beckman de 4 canais, utilizando-se derivações bipolares curtas (1mm com eletródios esféricos de platina. As experiências permitiram as seguintes conclusões: 1 — As características eletrofisiológicas dos fusos que ocorrem após lesões da formação reticular mesencefálica são muito semelhantes às dos fusos espontâneos e barbitúricos, inclusive quanto à projeção cortical. Quanto à duração dos potenciais que os constituem, contudo, notamos que a faixa de variação era mais centuada (20 a 80 ms, o que pode ser atribuído à maior complexidade dos potenciais do cérebro isolado, possivelmente pela falta de ação cronadora da formação reticular sobre o sistema sincronizador talâmico. 2 — Os mecanismos envolvidos na gênese dos fusos do sono barbitúrico ou espontâneo e os do cérebro isolado são, pelo menos em parte, dependentes do bloqueio da formação reticular mesencefálica. 3 — A formação reticular mesencefálica ativa preferencialmente o hemisfério cerebral homolateral; o contingente cruzado talvez seja mobilizado somente quando estímulos alertantes intensos atingem o tegmento mesencefálico. 4 — Além da formação reticular mesencefálica deve haver outros mecanismos ativadores corticais, visto que, em preparações agudas de cérebro isolado, observamos: a surtos de curta duração de atividade dessincronizada; b oscilações freqüentes do ECoG durante o registro

  17. Atributos químicos de um latossolo bruno sob sistema plantio direto em função da estratégia de adubação e do método de amostragem de solo Chemical properties of a haplohumox under no-tillage as related to fertilization strategies and soil sampling method

    Directory of Open Access Journals (Sweden)

    Volnei Pauletti


    greater in the no-tillage system because there is no homogenization of the soil surface layer. This study evaluated the effect of fertilization strategies on soil chemical properties at distinct soil depths after three and six years from the beginning of the experiment in a Haplohumox under long-term no-tillage. Besides, the variation of these properties considering two methods of soil sampling, with and without inclusion of the seeding row, was evaluated. Ten treatments were applied, nine considering P sources (rock phosphate and triple superphosphate, row preparation equipment (double disc opener and coulter opener, fertilizer placement (in-row and broadcast, time of application (summer and winter and one control treatment, without fertilizer. The measured soil chemical properties were higher in the surface layers and depth distribution, except for P, was not influenced by the fertilization strategies. The soil P values were the lowest in the 0-5 cm layer where the coulter opener equipment had been continuously used for more than three years and no fertilizer was applied. With the inclusion of the sowing row in the soil sampling, higher K, Ca and CEC values were observed. The methodologies of soil sampling, with and without the inclusion of the sowing row and the 0-10 and 0-20 cm sampling layers resulted in similar fertilizer and lime recommendations for areas under long-term no-tillage.

  18. Mieloma Múltiplo: 50 casos diagnosticados por citometria de fluxo Multiple Myeloma: 50 cases diagnosed by flow cytometry

    Directory of Open Access Journals (Sweden)

    Mariana F. A. Funari


    Full Text Available O Mieloma Mútiplo é uma doença de evolução heterogênea, na qual a maioria dos pacientes recai muito precocemente após o tratamento. Nesse contexto, o objetivo principal deste trabalho é relatar diferentes estratégias de análise do mieloma por citometria de fluxo e sua importância na associação com citogenética no diagnóstico de doença residual. Entre 2.450 casos de doenças onco-hematológicas estudados, de setembro de 1993 a agosto de 2004, foram diagnosticados 50 (2,0% Mieloma Múltiplo. Foram feitas análises morfológicas e, até o ano de 2000, as imunofenotipagens foram realizadas no citômetro de fluxo XL-MCL (Coulter pela estratégia tamanho/complexidade, utilizando os anticorpos monoclonais CD19, CD20, CD38, CD45, CD56, HLA-DR, kappa e lambda de superfície e intracitoplasmáticas. A partir de 2001 passaram-se a utilizar painéis seqüenciais através do histograma CD138/Complexidade e anticorpos monoclonais CD19, CD38, CD56, CD117, kappa e lambda intracitoplasmáticas. Mais recentemente foram incluídos no painel os anticorpos CD45, HLA-DR e CD33. A análise do DNA foi realizada por citometria com auxílio do programa Multicycle em nove amostras, sendo que sete apresentaram população aneuplóide. O cariótipo com banda G foi realizado em 25 casos, e a pesquisa de deleção do 13q por FISH em 15. Alterações cromossômicas foram encontradas em 4 casos, sendo duas deleções de 13q confirmadas por FISH. A mudança na estratégia de gates associada à citogenética e ao estudo da cinética do ciclo do DNA permitem melhor identificação de células plasmáticas anômalas, avaliação do prognóstico e detecção de doen��a residual.Multiple myeloma is a disease with variable natural history, but a clinical fact is that most of the transplanted patients relapse early after transplantation. Minimal residual disease should be one prognostic factor of transplanted patients that could judge if such patients are at risk

  19. Atividade elétrica cerebral do rato com lesões da formação reticular mesencefálica

    Directory of Open Access Journals (Sweden)

    Walter C. Pereira


    Full Text Available No presente estudo foram utilizados 73 ratos em preparações agudas e crônicas, nas quais lesamos a formação reticular mesencefálica com corrente contínua (3,5 a 4,0 mA durante 5 a 10 segundos. O eletródio ativo era implantado estereotàxicamente segundo as coordenadas de König e Klippel. As lesões eram feitas parcial ou totalmente, uni ou bilateralmente, e em todos os animais procedeu-se ao controle histológico das áreas lesadas, usando-se o método de Weil. O registro da atividade elétrica cortical foi feito com polígrafo Beckman de 4 canais, utilizando-se derivações bipolares curtas (1mm com eletródios esféricos de platina. As experiências permitiram as seguintes conclusões: 1 — As características eletrofisiológicas dos fusos que ocorrem após lesões da formação reticular mesencefálica são muito semelhantes às dos fusos espontâneos e barbitúricos, inclusive quanto à projeção cortical. Quanto à duração dos potenciais que os constituem, contudo, notamos que a faixa de variação era mais centuada (20 a 80 ms, o que pode ser atribuído à maior complexidade dos potenciais do cérebro isolado, possivelmente pela falta de ação cronadora da formação reticular sobre o sistema sincronizador talâmico. 2 — Os mecanismos envolvidos na gênese dos fusos do sono barbitúrico ou espontâneo e os do cérebro isolado são, pelo menos em parte, dependentes do bloqueio da formação reticular mesencefálica. 3 — A formação reticular mesencefálica ativa preferencialmente o hemisfério cerebral homolateral; o contingente cruzado talvez seja mobilizado somente quando estímulos alertantes intensos atingem o tegmento mesencefálico. 4 — Além da formação reticular mesencefálica deve haver outros mecanismos ativadores corticais, visto que, em preparações agudas de cérebro isolado, observamos: a surtos de curta duração de atividade dessincronizada; b oscilações freqüentes do ECoG durante o registro

  20. Enhanced UV Absorption in Carbonaceous Aerosols during MILAGRO and Identification of Potential Organic Contributors. (United States)

    Mangu, A.; Kelley, K. L.; Marchany-Rivera, A.; Kilaparty, S.; Gunawan, G.; Gaffney, J. S.; Marley, N. A.


    Measurements of aerosol absorption were obtained as part of the MAX-Mex component of the MILAGRO field campaign at site T0 (Instituto Mexicano de Petroleo in Mexico City) during the month of March, 2006 by using a 7- channel aethalometer (Thermo-Anderson). These measurements, obtained at 370, 470, 520, 590, 660, 880, and 950 nm at a 5 minute time resolution, showed an enhanced absorption in the UV over that expected from carbon soot alone. Samples of fine atmospheric aerosols (less than 0.1micron) were also collected at site T0 and T1 (Universidad Technologica de Tecamac, State of Mexico) from 5 am to 5 pm (day) and from 5 pm to 5 am (night) during the month of March 2006. The samples were collected on quartz fiber filters with high volume impactor samplers. The samples have been characterized for total carbon content (stable isotope ratio mass spectroscopy) and natural radionuclide tracers (210Pb, 210Po, 210Bi, 7Be, 13C, 14C, 40K, 15N). Continuous absorption spectra of these aerosol samples have been obtained in the laboratory from 280 to 900nm with the use of an integrating sphere coupled to a UV-visible spectrometer (Beckman DU with a Labsphere accessory). The integrating sphere allows the detector to collect and spatially integrate the total radiant flux reflected from the sample and therefore allows for the measurement of absorption on highly reflective or diffusely scattering samples (1). The continuous spectra also show an enhanced UV absorption over that expected from carbon soot and the general profiles are quite similar to those observed for humic and fulvic acids found as colloidal materials in surface and groundwaters (2), indicating the presence of humic-like substances (HULIS) in the fine aerosols. The spectra also show evidence of narrow band absorbers below 400 nm typical of polycyclic aromatics (PAH) and nitrated aromatic compounds. Spectra were also obtained on NIST standard diesel soot (SRM 2975), NIST standard air particulate matter (SRM 8785

  1. Infrared Absorption by Atmospheric Aerosols in Mexico City during MILAGRO. (United States)

    Kelley, K. L.; Mangu, A.; Gaffney, J. S.; Marley, N. A.


    Past research in our group using cylindrical internal reflectance spectroscopy has indicated that aqueous aerosols could contribute to the radiative warming as greenhouse species (1,2). Although aerosol radiative effects have been known for sometime and are considered one of the major uncertainties in climate change modeling, most of the studies have focused on the forcing due to scattering and absorption of radiation in the uv- visible region (3). Infrared spectral information also allows the confirmation of key functional groups that are responsible for enhanced absorption observations from secondary organics in the uv-visible region. This work extends our efforts to evaluate the infrared absorption by aerosols, particularly organics, that are now found to be a major fraction of urban and regional aerosols in the 0.1 to 1.0 micron size range and to help identify key types of organics that can contribute to aerosol absorption. During the MILAGRO campaign, quartz filter samples were taken at 12-hour intervals from 5 am to 5 pm (day) and from 5 pm to 5 am (night) during the month of March 2006. These samples were taken at the two super-sites, T-0 (Instituto Mexicano de Petroleo in Mexico City) and T-1 (Universidad Technologica de Tecamac, State of Mexico). The samples have been characterized for total carbon content (stable isotope mass spectroscopy) and natural radionuclide tracers, as well as for their UV-visible spectroscopic properties by using integrating sphere diffuse reflectance spectroscopy (Beckman DU with a Labsphere accessory). These same samples have been characterized in the mid and near infrared spectral ranges using diffuse reflection spectroscopy (Nicolet 6700 FTIR with a Smart Collector accessory). Aerosol samples were removed from the surfaces of the aerosol filters by using Si-Carb sampler. The samples clearly indicate the presence of carbonyl organic constituents and the spectra are quite similar to those observed for humic and fulvic acids

  2. The Canadian laboratory initiative on pediatric reference intervals: A CALIPER white paper. (United States)

    Adeli, Khosrow; Higgins, Victoria; Trajcevski, Karin; White-Al Habeeb, Nicole


    sex-specific pediatric reference intervals. The first CALIPER direct reference interval study was published in 2012, with age- and sex-specific reference intervals reported for 40 common biochemical markers. To date, CALIPER has collected health information and blood samples from over 9700 community children and adolescents, and has established a comprehensive database of age- and sex-specific reference intervals for over 100 biomarkers of pediatric disease. CALIPER has also performed a series of transference and verification studies to expand the applicability of the CALIPER database to five major analytical platforms, including Abbott, Beckman, Ortho, Roche, and Siemens. Through novel knowledge translation initiatives, the CALIPER Reference Interval Database has been made freely available online ( ) as well as on a mobile application (CALIPER Reference App), and it is used by clinical laboratories across Canada, the United States, and globally. In addition to establishing this comprehensive pediatric reference interval database, CALIPER has also performed a series of sub-studies, including examining how reference intervals are affected by pre-analytical factors (i.e. sample stability at specific storage conditions, fasting status and time of sample collection), biological variation (i.e. intraindividual and interindividual biological variation, reference change values), and ethnicity and pubertal development stage. In this white paper, extensive tables of pediatric reference intervals are provided for easy reference for clinical laboratories worldwide. All data reported have been published in over 20 peer reviewed publications and are also available through the CALIPER Reference Interval Database as well as the CALIPER Reference App for mobile devices.

  3. Association of single nucleotide polymorphism at position 45 in adiponectin gene with plasma adiponectin level and insulin resistance in obesity

    International Nuclear Information System (INIS)

    Chen Xiaoyu; Li Xisheng; Lin Xiahong; Gao Hongzhi; Li Qiulan; Zha Jinshun


    Objective: To explore the association of single nucleotide polymorphism at position 45 (SNP45) in adiponectin gene with plasma adiponectin level and insulin resistance in obesity in Quanzhou area of Fujian province. Methods: Two hundred and forty-eight patients with obesity and 225 normal control subjects were enrolled in this study.Fasting insulin (FINS) were measured by radioimmunoassay and fasting plasma glucose (FPG), total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C) were measured by BECKMAN DXC800 biochemistry analyzer. Body mass index (BMI), waist to hip ratio,homeostasis model assessment of insulin resistance (HOMA-IR) were calculated. Plasma adiponectin levels were examined by means of enzyme-linked immunosorbentassy. The adiponectin gene SNP45 was identified by PCR-restriction fragment length polymorphism. Results: (1) Frequencies of GG+GT genotype in obesity group and normal control group were 61% and 44% respectively (χ 2 =14.182, P<0.01), and G allele frequencies were 35% and 25% (χ 2 =10.708, P<0.01). (2) In obesity group,the subjects with SNP45 GG+GT genotype had higher TG and LDL-C levels than those with TT genotype (t=2.604, P<0.01; t=5.507, P<0.01), and had lower adiponectin level than those with TT genotype (t=2.275, P<0.05), and had significantly lower HDL-L level than those with TT genotype (t=10.100, P< 0.01). (3) In normal control group,the subjects with SNP45 GG +GT genotype had significantly lower adiponectin,TG,TC levels than those with TT genotype (t=2.510, P<0.05; t=2.922, P<0.01; t=3.272, P< 0.01). (4) Logistic analysis proved that the SNP45 GG+GT genotype in obesity group was associated with decreased risk of plasma adiponectin level (OR=0.810, 95% CI : 0.673-0.975, P<0.05), and with increased risk of HOMA-IR (OR=1.746, 95% CI : 1.060-2.875, P<0.05). The SNP45 GG+GT genotype in normal control group was associated with increased risk of HOMA-IR (OR=3

  4. The Argonne Leadership Computing Facility 2010 annual report.

    Energy Technology Data Exchange (ETDEWEB)

    Drugan, C. (LCF)


    that will be faster than petascale-class computers by a factor of a thousand. Pete Beckman, who served as the ALCF's Director for the past few years, has been named director of the newly created Exascale Technology and Computing Institute (ETCi). The institute will focus on developing exascale computing to extend scientific discovery and solve critical science and engineering problems. Just as Pete's leadership propelled the ALCF to great success, we know that that ETCi will benefit immensely from his expertise and experience. Without question, the future of supercomputing is certainly in good hands. I would like to thank Pete for all his effort over the past two years, during which he oversaw the establishing of ALCF2, the deployment of the Magellan project, increases in utilization, availability, and number of projects using ALCF1. He managed the rapid growth of ALCF staff and made the facility what it is today. All the staff and users are better for Pete's efforts.

  5. Implications of Climate Volatility for Agricultural Commodity Markets in the Presence of Biofuel Mandates (United States)

    Verma, M.; Diffenbaugh, N. S.; Hertel, T. W.; Beckman, J.


    In presence of bio-fuels, link between energy and agricultural commodity markets has become more complex. An increase in ethanol production to minimum 15bn gallons a year - Renewable Fuel Standard (RFS) and current technically permissible maximum 10% blending limit - Blend Wall (BW); make the link even stronger. If oil prices in future do not rise significantly from their current levels, this minimum production requirement would likely be binding. In such a scenario any fluctuation in crop production will have to be absorbed by the non-ethanol usage of the crop and would translate into crop prices adjusting to clear the markets and therefore the commodity prices will be more volatile. At high oil prices it is possible that the BW may become binding, severing the link between oil prices and commodity prices as well, potentially leading to higher price volatility. Hertel and Beckman (2010) find that, with both RFS and BW simultaneously binding, corn price volatility due to supply side shocks (which could arise from extreme climate events) could be more than 50% as large as in the absence of bio-fuel policies. So energy markets are important determinants of agricultural commodity price volatility. This proposal intends to introduce the increased supply side volatility on account of climate change and volatility, in the framework. Global warming on account of increased GHG concentrations is expected to increase the intensity and frequency of hot extremes in US (Diffenbaugh et al. 2008) and therefore affect corn yields. With supply shocks expected to increase, binding RFS and BW will exacerbate the volatility, while if they are non-binding then the price changes could be cushioned. We propose to model the impacts of climate changes and volatility on commodity prices by linking three main components - a. Projections for change in temperature and precipitation using climate model b. A statistical model to predict impacts of change in climate variable on corn yields in US

  6. Progress toward curing HIV infection with hematopoietic cell transplantation

    Directory of Open Access Journals (Sweden)

    Petz LD


    Full Text Available Lawrence D Petz,1 John C Burnett,2 Haitang Li,3 Shirley Li,3 Richard Tonai,1 Milena Bakalinskaya,4 Elizabeth J Shpall,5 Sue Armitage,6 Joanne Kurtzberg,7 Donna M Regan,8 Pamela Clark,9 Sergio Querol,10 Jonathan A Gutman,11 Stephen R Spellman,12 Loren Gragert,13 John J Rossi2 1StemCyte International Cord Blood Center, Baldwin Park, CA, USA; 2Department of Molecular and Cellular Biology, Irell and Manella Graduate School of Biological Sciences, 3Department of Molecular and Cellular Biology, Beckman Research Institute, City of Hope, Duarte, CA, USA; 4CCR5-Δ32/Δ32 Research Department, StemCyte International Cord Blood Center, Baldwin Park, CA, USA; 5Department of Stem Cell Transplantation, University of Texas MD Anderson Cancer Center, Houston, TX, USA; 6MD Anderson Cord Blood Bank, Department of Stem Cell Transplantation, University of Texas MD Anderson Cancer Center, Houston, TX, USA; 7Carolinas Cord Blood Bank, Duke University Medical Center, Durham, NC, USA; 8St Louis Cord Blood Bank, SSM Cardinal Glennon Children's Medical Center, St Louis, MO, USA; 9Enhance Quality Consulting Inc., Oviedo, FL, USA; 10Cell Therapy Service and Cord Blood Bank, Banc de Sang i Teixits, Barcelona, Spain; 11BMT/Hematologic Malignancies, University of Colorado, Aurora, CO, USA; 12Immunobiology and Observational Research, CIBMTR, Minneapolis, MN, USA; 13National Marrow Donor Program/Be The Match, Minneapolis, MN, USA Abstract: HIV-1 infection afflicts more than 35 million people worldwide, according to 2014 estimates from the World Health Organization. For those individuals who have access to antiretroviral therapy, these drugs can effectively suppress, but not cure, HIV-1 infection. Indeed, the only documented case for an HIV/AIDS cure was a patient with HIV-1 and acute myeloid leukemia who received allogeneic hematopoietic cell transplantation (HCT from a graft that carried the HIV-resistant CCR5-Δ32/Δ32 mutation. Other attempts to establish a cure for HIV

  7. Resistência mecânica do solo e força de tração em hastes sulcadoras de semeadoras-adubadoras em sistema de integração lavoura-pecuária Mechanical resistance of soil and traction force by planter chisel type openers in crop-livestock system

    Directory of Open Access Journals (Sweden)

    Osmar Conte


    . Treatments consisted of four grazing intensities, which resulted in pasture heights of 10; 20; 30 and 40 cm, and a no-grazed area used as blank, designed in randomized blocks with three replicates. After the grazing period and during soybean sowing, RP was evaluated; also, required power to openers chisel type coulter was measured. Both parameters increased with increments in grazing intensity. There was correlation between both parameters, showing that it is possible to evaluate the condition of soil´s compaction by measuring draft requirement by fertilizer furrow openers.

  8. P-gp Expression and Rh 123 Efflux Assay Have no Impact on Survival in Egyptian Pediatric Acute Lymphoblastic Leukemia Patients

    International Nuclear Information System (INIS)

    Kamel, A.M.; El-Sharkawy, N.; Yassin, D.; Shaaban, Kh.; Hussein, H.; Sidhom, I; Abo El-Naga, S.; Ameen, M.; El-Hattab, O.; Aly El-Din, N.H.


    In a previous work we have studied MDR status in terms of P-glycoprotein (P-gp) expression and Rhodamine 123 efflux assay in Egyptian acute leukemia patients. We have reported results comparable to the literature as regards ANLL both in pediatric and adult cases. However, higher figures were encountered for the functional assay in ALL. As our ALL cases especially in pediatric age group show worse prognosis compared to literature, we hypothesized that the higher percentage of cases with positive Rh 123 efflux assay might be a contributing factor. Material and Methods: A total of 108 cases were studied including 80 ALL and 28 ANLL. ALL cases included 48 male and 32 female with an age range of 6m to 18 yrs and a median of 7 yrs. ANLL cases included 18 male and 10 female with an age range of 6m to 18 yrs and a median of 8 yrs. P-gp expression was evaluated using 4E3 and DIC2 mAb, analyzed by Coulter XL flow cytometer and expressed as a ratio at a cut off of ≤1.1 and/or ≤5% positive cells. For the evaluation of MDR function Rh 123 efflux assay using cyclosporine as a blocker and expressed as a ratio at a cutoff of ≤1. 1 and/or ≤ 10% positive cells was performed. MDR expression and function were correlated to age, Hb, TLC, CD34 expression, immuno phenotype and DNA index in ALL, FAB subtypes in ANLL as well as to CR, DFS and EFS in ALL. In ALL, P-gp expression was encountered in 26.4% of cases. Positive Rh efflux was reported in 61.5%. No correlation was encountered between neither expression nor functional assay with age, Hb, TLC, CD34 expression or immuno phenotype. CR was achieved in 89.8%; neither P-gp expression nor Rh123 efflux had an impact on CR except for Rh 123 efflux in T-ALL where a cutoff of 1.25 could predict CR at a total accuracy of 70.6%. DFS was 92.3% while EFS was 72.2% for the whole group. No significant difference was encountered neither between cases expressing or lacking P-gp nor between cases with negative or positive Rh 123 efflux assay

  9. Analysis of changes in the percentage of B (CD19) and T (CD3) lymphocytes, nk cells, subsets CD4, CD8 in differentiated thyroid cancer patients treated with iodine-131

    International Nuclear Information System (INIS)

    Luo Quanyong; Yu Yongli; Chen Libo; Lu Hankui; Zhu Ruisen


    Objective: To evaluate the changes in the percentage of B (CD19) and T (CD3) lymphocytes, NK cells, subsets CD4, CD8 in patients with differentiated thyroid carcinoma (DTC) who received iodine-131 for therapeutic purposes. Methods: In this study, 102 DTC patients were divided into three groups. Group A, 8 cases received 1850 MBq of iodine-131 for the remnant thyroid ablation. Group B, 43 cases received 3700 MBq of iodine-131 for the treatment of cervical lymph node metastasis. Group C, 51 cases received 7400 MBq of iodine-131 for remote metastasis. All patients were in a hypothyroid state at the time of administration of iodine-131 and resumed L-thyroxine (2μg/Kg/day) 5 days after iodine-131 administration. The percentage of B and T lymphocytes, NK cells, subsets CD4, CD8 in peripheral blood were serially analyzed at baseline and at days 7, 30 and 90 after iodine-131 administration using a Coulter EPICS XL cytometer. Ten healthy individuals were used as a control group for lymphocyte subset values. Results: Comparing the basal lymphocyte subset levels in groups A, B and C with the control group, only NK cells showed significantly higher levels in patients than in controls (P=0.043). In group A, only the percentage of NK cells (P=0.031) and B cells (P =0.024) were reduced at day 7. In group B, a decrease in the percentage of NK cells at days 7(P=0.005), 30 (P=0.021) was observed, while a significant decrease in the percentage of B cells was only observed at day 7(P=0.006). Among T cells, only CD4+ was obviously affected, resulting in a reduction in the CD4+/CD8+ ratio at day 30 (P=0.034). In group C, patients showed a decrease in the percentage of NK cells at days 7 (P=0.023), 30 (P=0.006). A decrease in the percentage of both B and T lymphocytes was observed at days 7(P=0.020, 0.018 respectively), 30(P=0.041, 0.025 respectively). Among T cells, a decrease in the percentage of CD4+ and an increase in the percentage of CD8+ were observed, resulting in a marked

  10. The pyrethroid metabolites 3-phenoxybenzoic acid and 3-phenoxybenzyl alcohol do not exhibit estrogenic activity in the MCF-7 human breast carcinoma cell line or Sprague-Dawley rats

    International Nuclear Information System (INIS)

    Laffin, Brian; Chavez, Marco; Pine, Michelle


    Synthetic pyrethroids are one of the most frequently and widely used class of insecticides, primarily because they have a higher insect to mammalian toxicity ratio than organochlorines or organophosphates. The basic structure of pyrethroids can be characterized as an acid joined to an alcohol by an ester bond. Pyrethroid degradation occurs through either oxidation at one or more sites located in the alcohol or acid moieties or hydrolysis at the central ester bond, the latter reaction being important for mammalian metabolism of most pyrethroids. The primary alcohol liberated from the ester cleavage is hydroxylated to 3-phenoxybenzyl alcohol, which for most pyrethroids is then oxidized to 3-phenoxybenzoic acid. These products may then be conjugated with amino acids, sulfates, sugars, or sugar acids. In vitro studies have suggested that some of the pyrethroids may have estrogenic activity. Interestingly, the chemical structure of specific pyrethroid metabolites indicates that they may be more likely to interact with the estrogen receptor than the parent compounds. Two of the pyrethroid metabolites, 3-phenoxybenzoic acid (3PBA) and 3-phenoxybenzyl alcohol (3PBalc) have been reported to have endocrine activity using a yeast based assay. 3PBAlc exhibited estrogenic activity with reported EC 50 s of 6.67 x 10 -6 and 2 x 10 -5 while 3PBAcid exhibited anti-estrogenic activity with a calculated IC 50 of 6.5 x 10 -5 . To determine if the metabolites were able to cause the same effects in a mammalian system, the estrogen-dependent cell line, MCF-7, was utilized. Cells were treated with 1.0, 10.0 or 100.0 μM concentrations of each metabolite and cytotoxicity was assessed. The two lowest concentrations of both metabolites did not induce cell death and even appeared to increase proliferation over that of the control cells. However, when cellular proliferation was measured using a Coulter counter neither metabolite stimulated proliferation (1.0 nM, 10.0 nM, or 10.0 μM) or

  11. Desempenho de uma semeadora-adubadora direta, em função da velocidade de eslocamento e do tipo de manejo da biomassa da cultura de cobertura do solo Performance of a seeding-fertilizer machine in function of the displacement speed and soil cover crop biomass management

    Directory of Open Access Journals (Sweden)

    Klinger B. Branquinho


    Full Text Available O objetivo do presente trabalho foi estudar o desempenho de uma semeadora-adubadora, operando em duas velocidades sobre a palhada da cultura do milheto (Pennisetum glaucum (L. R. Brown, após diferentes manejos, na semeadura direta da cultura da soja (Glycine Max L.. O experimento foi realizado com seis tratamentos, em esquema fatorial, delineados em blocos ao acaso, com quatro repetições. Os tratamentos combinaram três manejos (triturador de palhas tratorizado, rolo- faca e herbicida com duas velocidades de deslocamento da semeadora-adubadora (5,2 e 7,3 km h-1. Foram analisadas as seguintes variáveis: acúmulo e decomposição de massa seca do milheto, índice de emergência da cultura da soja, uniformidade de distribuição longitudinal de sementes, capacidade de campo efetiva da semeadora-adubadora e produtividade da soja. Os resultados mostraram que não houve diferença na decomposição da massa de milheto após os manejos e que esses, juntamente com a velocidade de deslocamento do conjunto trator-semeadora-adubadora, não influenciaram no número de dias para a emergência das plântulas de soja e na distribuição longitudinal das sementes. A capacidade de campo efetiva da semeadora-adubadora foi maior na velocidade mais alta. O rendimento de grãos não diferiu significativamente nos tratamentos estudados.The objective of the present work was to evaluate three different managements of the Pennisetum glaucum (L. R. Brown crop and two-speed displacement in a no-tillage system of soybean (Glycine max L. crop on a typical Eutrophic Red Latosol. The experiment was arranged in six treatments, in a factorial array, in a randomized complete block design, with four replications. The treatments were constituted from three combination of crop managements (straw chopper, residue coulter and herbicide and two speed displacement of the seeding-fertilizer machine (5.2 and 7.3 km h-1. The following variables have been analyzed: emergency index of

  12. ZAP-70 as A Possible Prognostic Factor in Childhood Acute Lymphoblastic Leukemia

    International Nuclear Information System (INIS)



    Background: Zeta-chain-associated protein (ZAP- 70) is a 70kD adaptor protein that acts quickly after T cell activation to propagate signal. The role of ZAP-70 in Tcell function is well established, and in the previous years, this molecule was considered to be T-cell specific. More recent data have documented a role of ZAP-70 in B cells. Interest in ZAP-70 has grown since it has been shown, through gene expression profiling, that it is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL). Purpose: The aim of this study was to investigate the expression of ZAP-70 in leukemic blasts of 50 newly diagnosed patients of B-lineage acute lymphoblastic leukemia (ALL), and to assess the correlation between ZAP-70 expression and various prognostic factors and outcome. Patients and Methods: This study included 50 pediatric patients with newly diagnosed B-lineage ALL. They were 28 males (56%) and 22 females (44%) presented to the Pediatric Oncology Department, National Cancer Institute, Cairo University, during the period from 2005 to 2007. The age range was 2 to 17 years with a mean of 8.58±5.8 years and median 8 years. All patients were subjected at presentation to a full clinical history and physical examination. Patients diagnosed with ALL were enrolled on St. Jude Total XV protocol: standard risk and low risk according to results of primary investigation. Immunophenotyping was done using monoclonal antibodies which were analyzed on Coulter XL (Panel included CD1, CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD19, CD22, Cytoplasmic m, anti k, anti l, CD13, CD33, anti classII MHC and TdT). Cases were considered ZAP-70 positive when exhibiting a ZAP/GAPDH (Glyceraldehyde- 3-phosphate dehydrogenase) ratio ³0.13. Results: The study revealed expression of ZAP-70 in 5/50 cases (10%). There was no statistically significant relation between ZAP-70 expression and the following: age, Total Leukocytic Count, hepatomegaly and splenomegaly. There

  13. Essai de classement typo-technologique des araires à partir des pièces métalliques découvertes en Gaule romaine en vue de leur reconstitution Setting up of a typological-technological classification method to try to reconstruct ards from metallic pieces found in Roman Gaul

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    André Marbach


    positions? A method of research has been developed from a fine analysis of the metallic pieces listed in a catalogue of these pieces for Gaul. The shares have been classified according to their working surface, the form of their sockets and the angle of the wearing surface with the ground if it is observable. As for the tanged shares the filing is similar, the length of the tang has however been taken into account here. From the values known for these pieces and a technical study with modelisation of the tilling implement, the possibility of a reconstruction of the ard has been explored. Researchers are here reminded of how important it is to publish the description of the pieces found with the utmost precision for the drawing as well as for the dimensions. As far as ards fitted with a socket share are concerned uncertainty prevails except for shares with a small working surface which, generally, are single ards (manche-sep. The reconstruction of tanged share-ards seems possible including the length of the draught-beam (chambige. Several concrete examples of reconstructed ards have been studied. Thus, if the reconstruction of a tanged share from metallic pieces, tang and coulter may be envisaged, that of iron share ards is uncertain except for plough-ards (reversible.

  14. Stem cell harvesting protocol research in autologous transplantation setting: Large volume vs. conventional cytapheresis

    Directory of Open Access Journals (Sweden)

    Balint Bela


    Full Text Available Background/Aim. The use of peripheral blood as a source of hematopoietic stem cells (SCs is progressively increasing and has nearly supplanted bone marrow transplantation. Interpatient variability in the degree and kinetics of SC mobilization into peripheral blood is an expected event after conventional chemotherapy-based treatment, followed by sequential administration of recombinant granulocyte-colony- stimulating factor (rHu-CSF. In this study, specific factors associated with the application of two different SC-harvesting approaches, including the use of large volume leukapheresis (LVL vs. repetitive conventional apheresis (RCA, were analyzed. The basic goal of the study was to evaluate the influence of apheresis protocol (collection timing, processed blood volume and cell yield upon the clinical outcome of transplantation. Methods. Results obtained by LVL (76 pts and RCA (20 pts - control group were compared. The SC mobilizing regimen used was cyclophosphamide (4-7 g/m2 or polychemotherapy and rHuG-CSF 10-16 μg/kg of body mess (bm per day. Cell harvesting was performed using COBE-Spectra (Caridian-BCT, USA. The volume of processed blood in LVL setting was ≥ 3.5 - fold of the patient's circulating blood quantity (ranged from 12.7 to 37.8 l. All patients tolerated well the use of intensive treatment, without any side or adverse effects. Our original controlled-rate cryopreservation was carried out with 10% dimethyl sulfoxide (DMSO using Planer R203/200R or Planer 560-16 equipments (Planer Products Ltd, UK. Total nucleated cell (NC and mononuclear cell (MNC counts were examined by flow cytometry (Advia-2120 Bayer, Germany; Technicon H-3 System, USA. The CD34+ cell surface antigen was investigated by the EPICS XL-MCL device (Coulter, Germany. Results. Performing LVL-apheresis, high-level MNC and CD34+ cell yields (7.6±4.6 × 108/kg bm and 11.8±6.5 × 106/kg bm, respectively were obtained. As a result, rapid hematopoietic reconstitution

  15. Parâmetros solo-máquina em função de doses de resíduos vegetais e profundidades de deposição de adubo em semeadura direta Parameters of soil-machine in function of the amount of cover residues and different fertilizer deposition depths in direct drilling

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    Karina M. Kamimura


    Full Text Available O tráfego contínuo e inadequado de máquinas em solos sob semeadura direta tem provocado alterações dos atributos físicos e mecânicos dos solos, influenciando, dessa forma, na produtividade das culturas. O experimento foi conduzido na Estação Experimental Agronômica da Universidade Federal do Rio Grande do Sul, no município de Eldorado do Sul - RS, num Argissolo Vermelho distrófico típico, com o objetivo de quantificar a força de tração e obter informações sobre os atributos físicos do solo, em semeadura direta sobre resíduos de aveia-preta e ervilhaca parcialmente decomposta. O delineamento experimental utilizado foi em blocos casualizados, com parcelas subsubdivididas, sendo, nas parcelas principais, doses de resíduos da cultura de inverno (0; 1,3; 2,6; 3,2; 3,8 e 5,1 Mg ha-1 de palha de aveia-preta consorciada com ervilhaca, nas subparcelas profundidades de atuação da haste sulcadora de adubo (0,06 e 0,12 m e nas subsubparcelas tráfegos dos rodados do trator e colhedora. Os atributos físicos do solo foram afetados pelo tráfego dos rodados da máquina. A força de tração foi influenciada pela profundidade de atuação das hastes sulcadoras e o tráfego dos rodados.The continuous and inadequate machine traffic on soils under direct drilling has been changing the soil physical and mechanical properties, influencing therefore crop productivity. The experiment was carried out at the Agricultural Research Station of the Universidade Federal do Rio Grande do Sul (Eldorado do Sul, RS, Brazil on Typic Paleudlt. The main aim of the work was to measure coulter draft requirements and physical attributes under black oats (Avena strigosa Schieb and common vetch (CV (Vicia sativa L. partially decomposed. The experimental design was carried out in randomized blocks, with split-split-plots and three repetitions. The main treatments were plots with different amount of residues of the winter crop (0, 1,3; 2,6; 3,2; 3,8 and 5,1 Mg ha-1


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    Samo Zver


    Full Text Available Background. ALL is a malignant blood disease and lymphoblasts have origin in B or T lymphatic cell line. In 1997 established new World Health Organisation classification (WHO classification of malignant haematological diseases realizes the importance of cellular immunological markers (immunophenotype and chromosomal abnormalities (cytogenetics. Based on both findings we may distribute the patients in low, intermediate and high risk groups and the outcome of such distribution is risk adopted ALL treatment strategy. On Clinical department of haematology (CDH we have decided to overview immunophenotype characteristics of all ALL patients during the January 1, 1995–December 31, 2001 period.Methods and results. During the January 1, 1995–December 31, 2001 period on CDH we have treated 44 patients: 22 males and 22 females. With flow cytometer Coulter Epics XL MCS we have performed first primary antibody panel for acute leukemias antigens (CD2, CD7, CD10, CD19, CD34, cCD3, cCD13, cCD22, MPO, TdT, followed by secondary panel. The later have included antigen CD20, CD23, membrane and/or cytoplasmatic immunoglobulins or their light chains monoclonal antibodies for B-ALL and antigen CD3, CD4, CD5 and CD8 monoclonal antibodies for T-ALL subsets. Besides immunophenotyping we have evaluated all ALL patients also morphologically according to FAB classification (French-AmericanBritish classification, which is an old classification based solely on morphology.32/44 (73% patients have had immunophenotypic B-ALL, and 12/44 (27% T-ALL. Subgroups distribution of B-ALL immunophenotype were: pro-B 4/44 (9%, »common«-B 18/44 (41%, pre-B 5/44 (11.5% and mature B 5744 (11.5% and for T-ALL immunophenotype were: pro-T 2/44 (4.5%, pre-T 5/44 (11.5%, cortical-T 4/44 (9% and mature-T 1/44 (2%. Our results are quite comparable with the available data from the literature, despite the fact that newest immunological markers such as CD1a, CD79a and CD22 were not available

  17. The role of versican G3 domain in regulating breast cancer cell motility including effects on osteoblast cell growth and differentiation in vitro – evaluation towards understanding breast cancer cell bone metastasis

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    Du William


    Full Text Available Abstract Background Versican is detected in the interstitial tissues at the invasive margins of breast carcinoma, is predictive of relapse, and negatively impacts overall survival rates. The versican G3 domain is important in breast cancer cell growth, migration and bone metastasis. However, mechanistic studies evaluating versican G3 enhanced breast cancer bone metastasis are limited. Methods A versican G3 construct was exogenously expressed in the 66c14 and the MC3T3-E1 cell line. Cells were observed through light microscopy and viability analyzed by Coulter Counter or determined with colorimetric proliferation assays. The Annexin V-FITC apoptosis detection kit was used to detect apoptotic activity. Modified Chemotactic Boyden chamber migration invasion assays were applied to observe tumor migration and invasion to bone stromal cells and MC3T3-E1 cells. Alkaline phosphatase (ALP staining and ALP ELISA assays were performed to observe ALP activity in MC3T3-E1 cells. Results In the four mouse breast cancer cell lines 67NR, 66c14, 4T07, and 4T1, 4T1 cells expressed higher levels of versican, and showed higher migration and invasion ability to MC3T3-E1 cells and primary bone stromal cells. 4T1 conditioned medium (CM inhibited MC3T3-E1 cell growth, and even lead to apoptosis. Only 4T1 CM prevented MC3T3-E1 cell differentiation, noted by inhibition of alkaline phosphatase (ALP activity. We exogenously expressed a versican G3 construct in a cell line that expresses low versican levels (66c14, and observed that the G3-expressing 66c14 cells showed enhanced cell migration and invasion to bone stromal and MC3T3-E1 cells. This observation was prevented by selective EGFR inhibitor AG1478, selective MEK inhibitor PD 98059, and selective AKT inhibitor Triciribine, but not by selective JNK inhibitor SP 600125. Versican G3 enhanced breast cancer cell invasion to bone stromal cells or osteoblast cells appears to occur through enhancing EGFR/ERK or AKT signaling

  18. The subglacial Lake Vostok (East Antarctica) surface snow is Earth-bound DNA (and dust)-free (United States)

    Bulat, S.; Marie, D.; Bulat, E.; Alekhina, I.; Petit, J.-R.


    The objective was to assess the microbial cell abundance in the surface snow in Central East Antarctica and the fate of microbial genomic DNA during summer short-time exposure to surface climatic (and radiation) conditions at Vostok using flow cytometry and DNA-based methods. The surface snow (until 4m deep) was collected as clean as possible in the vicinity of the Vostok station (3 sites - courtesy of A Ekaykin and ASC Lebedev Physical Iinstitute RAS) and towards the Progress station (4 more sites with one just 29km from the coast - courtesy of A Ekaykin and S Popov) in specially decontaminated plastic crates or containers of various volumes (up to 75 kg of snow). All subsequent snow treatment manipulations (melting, concentrating, genomic DNA extraction, primary PCR set up) were performed in clean room laboratory facilities (LGGE, UJF-CNRS, Grenoble, France). Cell concentrations were determined on meltwater aliquots prepared under clean room conditions using flow cytofluorometry (Biostation, Roscoff, France). The highly concentrated meltwater (until 10000 times down) was used to extract gDNA which were subjected to bacterial 16S rRNA genes amplification in PCR and sequencing. The gDNA of a complex mesophile microbial community for exposure trials were also prepared and put onto a filter under strict clean room conditions. The filters were got exposed open to solar radiation and surface temperature at Vostok during January for various time duration periods (from 25 to 1 day). As a result no microbial cells were confidently detected in surface snow samples differed by sampling sites and people asked to collect as well. Complementary the mineral dust particle abundance did not exceed 16 mkg per liter with the particle size mode about 2.5 mkm as shown using Coulter counter. Preliminary amongst the microparticles no unusual findings (e.g. spherules of cosmic origin) were observed by shape and element composition using electron scanning microscopy. The gDNA studies

  19. Engaging Patients and Caregivers Managing Rare Diseases to Improve the Methods of Clinical Guideline Development: A Research Protocol. (United States)

    Khodyakov, Dmitry; Kinnett, Kathi; Grant, Sean; Lucas, Ann; Martin, Ann; Denger, Brian; Peay, Holly; Coulter, Ian; Fink, Arlene


    guideline development process. We will solicit input on these best practice from Duchenne patients, caregivers, and clinicians by conducting a series of round-table discussions and making a presentation at an annual conference on Duchenne. The study protocol was reviewed by RAND's Human Subjects Protection Committee, which determined it to be exempt from review. Interviews with RAM experts have been completed. The projected study completion date is May 2020. We expect that the new method will make it easier to engage large numbers of patients and caregivers in the process of guideline development in a rigorous and culturally appropriate manner that is consistent with the way clinicians participate in guideline development. Moreover, this project will develop best practices that could help involve patients and caregivers in the clinical guideline development process in other clinical areas, thereby facilitating the work of guideline developers. ©Dmitry Khodyakov, Kathi Kinnett, Sean Grant, Ann Lucas, Ann Martin, Brian Denger, Holly Peay, Ian Coulter, Arlene Fink. Originally published in JMIR Research Protocols (, 28.04.2017.

  20. A special year, thanks and a look ahead (United States)

    Birch, David J. S.


    Dear authors, reviewers and readers of Measurement Science and Technology , I would like to thank all those who have published papers with us in 2013, and offer a special thanks to those of you who have given up much of your precious time and kindly reviewed articles for the journal. 2013 was of course a very special year for the journal, marking the 90th anniversary of the journal's foundation, as Journal of Scientific Instruments. In order to mark the anniversary we started the year with a historical perspective of the journal [1], and a historical review of 90 years of dielectric properties measurement of materials [2]. The celebrations continued with a stimulating meeting at the Institute of Physics in London in March, entitled 'Frontiers of Measurement'. In keeping with the multidisciplinary ethos of the journal, the speakers covered a diverse range of topics, all linked by the common language of measurement science. Patrick Gill of NPL talked about atomic clocks, Bruce Tromberg of the Beckman Laser Institute and Medical Clinic covered biophotonics, Roger Bisby of the University of Salford lectured on developments in biochemical techniques, Ivan Marusic of the University of Melbourne spoke on fluids and Mervyn Miles of the University of Bristol described recent developments in atomic force microscopy. Nicola Gulley, Editorial Director of IOP Publishing (IOPP), briefed the meeting on some of the latest developments in the world of scientific publishing. The meeting ended with a presentation to Sharon D'Souza, outgoing Publisher of the journal, and a welcome for Ian Forbes who has taken over the role. On behalf of all the Editorial Board I would like to offer a very special thanks to Sharon for all her great work for the journal over many years and wish her every success in her new IOPP posts as Publisher of Reports in Progress in Physics and the recently launched Methods and Applications in Fluorescence. Ian Forbes comes to the journal with a PhD in Physics

  1. Preparo do solo em áreas de produção de grãos, silagem e pastejo: efeito na resistência tênsil e friabilidade de agregados Soil tillage in grain and silage-producing areas and pasture: effect on tensile strength and aggregate friability

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    Marina Araújo Bavoso


    ção da F. Os resultados demonstraram que a RT foi influenciada pelos sistemas de produção e tipos de preparo do solo. O menor valor de RT foi verificado no solo sob Pastejo, enquanto o Sistema Plantio Direto apresentou o maior valor de RT. A friabilidade não distinguiu os sistemas de produção e tipos de preparo de solo estudados.The understanding and quantification of soil use and management on the soil physical quality are important to develop and select sustainable agricultural systems. The purpose of this study was to evaluate the structural quality of a clayey Brazilian Oxisol under different production and soil tillage systems by measuring the tensile strength (TS and to quantify aggregate friability (F. The study was conducted in Castro, Paraná state, in southern Brazil, using three production systems and seven soil tillage types, in a randomized block design. The following production systems were evaluated: (PS I - ryegrass for soil cover (winter and corn for grain production (summer; (PS II ryegrass for haylage/silage and corn for grain production; and (PS III - ryegrass for grazing and corn for grain production. Seven types of soil tillage were evaluated in each system: (G1 - plow harrow in winter, depth 0.15 m; (G2 - plow harrow in winter and summer, depth 0.15 m; (Plow - disk plow, depth 0.20 m; (Laser wing subsoiler - subsoiler wing tips, to a depth of 0.45 m; (Subsoiler - subsoiler, depth 0.80 m; (Aerator - soil aerater Aeromix®; and (NT - No-tillage, where ryegrass was sown using a double disc and corn with a coulter. From each experimental plot soil blocks (0.20 x 0.15 x 0.07 m were taken which were broken up by hand in their natural aggregates. Then, the aggregates were air-dried for 24 h and passed through sieves with diameters of 12.5 and 19 mm. Forty aggregates from each block were selected and tested for indirect tension to determine TS and quantify F. TS was influenced by the production systems and soil tillage types. The lowest and the

  2. Phase 1 Final Technical Report - MgB2 Synthesis: Pushing to High Field Performance

    International Nuclear Information System (INIS)

    Bhatia, Mohit; McIntyre, Peter


    crystalline boron results in the formation of parasitic phases such as MgB4, MgB7, etc. Such parasitic phases are a primary element of the connectivity problem, in which even though a sample powder may contain grains of high-quality MgB2, adjacent grains are surrounded by intergrowths of parasitic phases so that current trans-port is badly degraded. The best results to date have been obtained using boron powder produced long ago for a rocket propellant development project. The synthesis process was complex and is now largely lost, and the manufacturing equipment has long since been scrapped. The last batch of the powder has been used during recent years to support MgB2 R and D at several labs, but supplies are dwindling. ATC has identified a first application of its plasma torch to synthesize phase-pure amorphous boron flake using a rapid-quench splat technique. Inexpensive technical-grade boron would be purified of contaminants, then dispersed as an aerosol in inert gas and passed through the plasma torch to melt it into a spray. The spray would be splat-condensed on a rotating drum to form pure amorphous flake. The process would begin with technical-grade boron powder, having good stoichiometric purity, nanoscale particles, but significant contamination of MgO and crystalline boron. We used wet chemistry to remove B2O3 completely and reduced the MgO impurity, and analyzed the particle size distribution using a Coulter counter and the phase composition using X-ray diffrac-tion (XRD). The next step will be to build an rf plasma torch with a recirculating single-component aerosol feed and the cooled splat drum and collector, and undertake process devel-opment for amorphous boron powder. This revised goal has two benefits. First, it is an easier technology than our ultimate goal of a multi-component laminar flow torch. We have been counseled by those experienced in plasma torch technology that our ultimate goal will require a torch that should be feasible but has never been

  3. Increasing energy efficiency by geometric modification of hoe-type furrow opener

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    R Rahimzadeh


    Full Text Available Introduction Direct planting becomes more common in the recent years, because it conserves soil and water as well as it saves energy and time. However, this technology needs special implements such as seed planter. Given that direct planting is practiced in undisturbed lands, so it was needed to design a special furrow opener. In order to obtain a suitable furrow opener this experiment was conducted in rain-fed Agricultural Research Institute in Maragheh. Materials and Methods Most of seed planters that are used for cultivation in rain fed conditions are equipped by hoe-type furrow opener. Hoe-type furrow openers have good penetration in hard and dry soils. However, they do not have the ability for direct planting. Hoe-type furrow opener was chosen as a model. Then by changing the geometric form of the depth to width ratio (d/w, the two openers were designed. In the first design, which was called O1 two wings and a narrow blade acting as a coulter were added in front of the hoe-type furrow opener. In the second design, which was called O2, in addition to the O1 modification, furrow opener width was decreased and a disk blade was added for seed sowing (Fig. 1. The performance of O1 and O2 openers were compared with the conventional hoe-type furrow opener (check in soil bin and in field conditions. At three different forward speeds (1, 1.5 and 2 m.s-1 with 3 replications, the effects of the openers designs of vertical and horizontal soil forces were evaluated in soil-bin conditions. In order to evaluate the performance of the furrow opener in field conditions, an experiment was conducted using a split plot design based on RCBD at 4 replications. Furrow openers formed the main plots and forward speeds formed the sub plots. Each plot size was 22 meters long in two rows for each treatment. After germination of wheat crop, the numbers of seedlings in two rows were counted (along a one meter. After crop maturity, all plots were harvested by hand and

  4. Preface: SciDAC 2009 (United States)

    Simon, Horst


    and posters goes to the teams of researchers, the success of this year's conference is due to the strong efforts and support from members of the 2009 SciDAC Program Committee and Organizing Committee, and I would like to extend my heartfelt thanks to them for helping to make the 2009 meeting the largest and most successful to date. Program Committee members were: David Bader, LLNL; Pete Beckman, ANL; John Bell, LBNL; John Boisseau, University of Texas; Paul Bonoli, MIT; Hank Childs, LBNL; Bill Collins, LBNL; Jim Davenport, BNL; David Dean, ORNL; Thom Dunning, NCSA; Peg Folta, LLNL; Glenn Hammond, PNNL; Maciej Haranczyk, LBNL; Robert Harrison, ORNL; Paul Hovland, ANL; Paul Kent, ORNL; Aram Kevorkian, SPAWAR; David Keyes, Columbia University; Kwok Ko, SLAC; Felice Lightstone, LLNL; Bob Lucas, ISI/USC; Paul Mackenzie, Fermilab; Tony Mezzacappa, ORNL; John Negele, MIT; Jeff Nichols, ORNL; Mike Norman, UCSD; Joe Oefelein, SNL; Jeanie Osburn, NRL; Peter Ostroumov, ANL; Valerio Pascucci, University of Utah; Ruth Pordes, Fermilab; Rob Ross, ANL; Nagiza Samatova, ORNL; Martin Savage, University of Washington; Tim Scheibe, PNNL; Ed Seidel, NSF; Arie Shoshani, LBNL; Rick Stevens, ANL; Bob Sugar, UCSB; Bill Tang, PPPL; Bob Wilhelmson, NCSA; Kathy Yelick, NERSC/LBNL; Dave Zachmann, Vista Computational Technology LLC. Organizing Committee members were: Communications: Jon Bashor, LBNL. Contracts/Logistics: Mary Spada and Cheryl Zidel, ANL. Posters: David Bailey, LBNL. Proceedings: John Hules, LBNL. Proceedings Database Developer: Beth Cerny Patino, ANL. Program Committee Liaison/Conference Web Site: Yeen Mankin, LBNL. Tutorials: David Skinner, NERSC/LBNL. Visualization Night: Hank Childs, LBNL; Valerio Pascucci, Chems Touati, Nathan Galli, and Erik Jorgensen, University of Utah. Again, my thanks to all. Horst Simon San Diego, California June 18, 2009

  5. Without 'Focus'

    Directory of Open Access Journals (Sweden)

    Aldo Sevi


    Full Text Available It is widely accepted that a notion of 'focus', more or less as conceived of in Jackendoff (1972, must be incorporated into our theory of grammar, as a means of accounting for certain observed correlations between prosodic facts and semantic/pragmatic facts. In this paper, we put forth the somewhat radical idea that the time has come to give up this customary view, and eliminate 'focus' from our theory of grammar. We argue that such a move is both economical and fruitful.Research over the years has revealed that the correlations between prosody, 'focus', and the alleged semantic/pragmatic effects of focus are much less clear and systematic than we may have initially hoped. First we argue that this state of affairs detracts significantly from the utility of our notion of 'focus', to the point of calling into question the very motivation for including it in the grammar. Then we look at some of the central data, and show how they might be analyzed without recourse to a notion of 'focus'. We concentrate on (i the effect of pitch accent placement on discourse congruence, and (ii the choice of 'associate' for the so-called 'focus sensitive' adverb only. We argue that our focus-free approach to the data improves empirical coverage, and begins to reveal patterns that have previously been obscured by preconceptions about 'focus'.ReferencesBeaver, D. & Clark, B. 2008. Sense and Sensitivity: How Focus Determines Meaning. Blackwell.Beaver, D., Clark, B., Flemming, E., Jaeger, T. F. & Wolters, M. 2007. ‘When semantics meets phonetics: Acoustical studies of second occurrence focus’. Language 83.2: 245–76., M. & Hirschberg, J. 1994. ‘The ToBI Annotation Conventions’. Ms.,, D. 1972. ‘Accent is predictable (if you are a mind-reader’. Language 48.3: 633–44.üring, D. 2006. ‘Focus projection and default

  6. Preface: SciDAC 2008 (United States)

    Stevens, Rick


    Microsoft Research at a new conference facility in Redmond, Washington. Over 90 people attended the tutorials, which covered topics ranging from an introduction to BG/P programming to advanced numerical libraries. The SciDAC and INCITE programs and the DOE Office of Advanced Scientific Computing Research core program investments in applied mathematics, computer science, and computational and networking facilities provide a nearly optimum framework for advancing computational science for DOE's Office of Science. At a broader level this framework also is benefiting the entire American scientific enterprise. As we look forward, it is clear that computational approaches will play an increasingly significant role in addressing challenging problems in basic science, energy, and environmental research. It takes many people to organize and support the SciDAC conference, and I would like to thank as many of them as possible. The backbone of the conference is the technical program; and the task of selecting, vetting, and recruiting speakers is the job of the organizing committee. I thank the members of this committee for all the hard work and the many tens of conference calls that enabled a wonderful program to be assembled. This year the following people served on the organizing committee: Jim Ahrens, LANL; David Bader, LLNL; Bryan Barnett, Microsoft; Peter Beckman, ANL; Vincent Chan, GA; Jackie Chen, SNL; Lori Diachin, LLNL; Dan Fay, Microsoft; Ian Foster, ANL; Mark Gordon, Ames; Mohammad Khaleel, PNNL; David Keyes, Columbia University; Bob Lucas, University of Southern California; Tony Mezzacappa, ORNL; Jeff Nichols, ORNL; David Nowak, ANL; Michael Papka, ANL; Thomas Schultess, ORNL; Horst Simon, LBNL; David Skinner, LBNL; Panagiotis Spentzouris, Fermilab; Bob Sugar, UCSB; and Kathy Yelick, LBNL. I owe a special thanks to Mike Papka and Jim Ahrens for handling the electronic theater. I also thank all those who submitted videos. It was a highly successful experiment. Behind the

  7. Browning boreal forests of western North America (United States)

    Verbyla, David


    's boreal forest Remote Sens. 2 2729-47 Riordan B, Verbyla D and McGuire A D 2006 Shrinking ponds in subarctic Alaska based on 1950-2002 remotely sensed images J. Geophys. Res. 111 G04002 Ruess R W, McFarland J M, Trummer L M and Rohrs-Richey J K 2009 Disease-mediated declines in N-fixation inputs by Alnus tenuifolia to early-successional floodplains in interior and south-central Alaska Ecosystems 12 489-502 Stafford J M, Wendler G and Curtis J 2000 Temperature and precipitation of Alaska: 50 year trend analysis Theor. Appl. Climatology 67 33-44 Stow D, Peterson A, Hope A, Engstrom R and Coulter L 2007 Greenness trends of Arctic tundra vegetation in the 1990s: comparison of two NDVI data sets from NOAA AVHRR systems Int. J. Remote Sens. 28 4807-22 van Mantgem P J et al 2009 Widespread increase of tree mortality rates in the western United States Science 323 521-4 Walsh, J E, Chapman W L, Romanovsky V, Christensen J H and Stendel M 2008 Global climate model performance over Alaska and Greenland J. Clim. 21 6156-74 Wendler G and Shulski M 2009 A century of climate change for Fairbanks, Alaska Arctic 62 295-300 Zhang K, Kimball J S, Hogg E H, Zhao M, Oechel W C, Cassano J J and Running S W 2008 Satellite-based model detection of recent climate-driven changes in northern high-latitude vegetation productivity J. Geophys. Res. 113 G03033

  8. Clinical Investigations. T-Lymphocyte Subset Absolute Counts in the Peripheral Blood of Mediterranean Spotted Fever Patients: Relations to Disease Severity / Абсолютное Количество Субпопуляций Т-Лимфоцитов В Перифери- Ческой Крови Пациентов Со Средиземноморской Пятнистой Лихо- Радкой: Установление Связи С Тяжестью Заболевания

    Directory of Open Access Journals (Sweden)

    Baltadzhiev Ivan G.


    Full Text Available Возбудителем средиземноморской пятнистой лихорадки (СПЛ в Болгарии является Rickettsia conorii. ЦЕЛЬ: Целью настоящего исследования является установление абсолютного количества субпопуляций Т-лимфоцитов и установление связи данного количества с тяжестью заболевания. МАТЕРИАЛЫ И МЕТОДЫ: Абсолютное количество субпопуляций Т-лимфоцитов было исследовано в крови 62 пациентов в остром стадии СПЛ. Пациенты были распределены в три идентичные по возрасту и полу группы в соответствии с тяжестью заболевания: лёгкой, средней тяжести и тяжёлой формы. В качестве контрольной группы были обследованы 32 здоровые лица, идентичные по возрасту и полу с больными. Диагноз был подтверждён при помощи иммунофлюоресцентного метода. Иммунофенотипирование было осуществлено с применением флоуцитометра Epics XL- MCL Coulter, USA. РЕЗУЛЬТАТЫ: Абсолютное количество как иммунокомпетентных Т-клеток (CD3+, так и субпопуляций хелперов/индукторов (CD3+CD4+ и супрессоров/цитотоксинов (CD3+CD8+ понижается при тяжёлых формах СПЛ. Субпопуляции наивных Т-лимфоцитов (CD4+CD45RA+, как и активированных Т-клеток памяти (CD4+CD45RO+ у

  9. only: A Case Study In Projective Meaning

    Directory of Open Access Journals (Sweden)

    Craige Roberts


    Full Text Available I offer an integrated theory of the meaning of only in which the prejacent, while not presupposed, is both entailed and backgrounded, hence tends to project (following a general proposal about projection due to Simons et al. 2010. Moreover, I argue, contra Beaver & Brady (2008, that only is not conventionally associated with focus, the focus effects arising instead pragmatically. But I do adopt aspects of their semantics for only, including the presupposition of a pre-order over the elements of its domain.ReferencesAbbott, B. 2000. ‘Presuppositions as non-assertions’. Journal of Pragmatics 32: 1419–37., B. 2008. ‘Presuppositions and common ground’. Linguistics and Philosophy 31: 523–538., P. 2010. ‘Entailment, assertion, and textual coherence: the case of almost and barely’. Linguistics 48, no. 3: 525–545., P., Roberts, C. & Allyn Smith, E. 2008. ‘Review of The Logic of Conventional Implicature by Chris Potts’. Linguistics and Philosophy 30: 707–749., J. D. 1993. ‘The importance of being “only”: testing the neo-Gricean versus neoentailment paradigms’. Journal of Semantics 10: 301–18., D. 2001. Presupposition and Assertion in Dynamic Semantics. CSLI Publications, Stanford, CA.Beaver, D. 2010. ‘Handout from a talk on projective meaning’. Stuttgart, Germany.Beaver, D. & Brady, C. 2003. ‘Always and Only: Why not all focus sensitive operators are alike’. Natural Language Semantics 11: 323–62., D. & Brady, C. 2008. Sense and Sensitivity. Blackwell., M. E. & Ayers, G. M. 1994. ‘Guidelines for ToBI labeling guide, ver. 2.0’. On-line at The Ohio State University: www