Sample records for bk010044 beckman coulter

  1. Beckman-coulter AC.T5diff血液分析仪复检规则的制定与评价%Establishment and Application of the Microscope Review Criteria for the Beckman-coulter AC.T 5diff Hematology Analyzer

    Institute of Scientific and Technical Information of China (English)

    王永锋; 尤涛; 康炜


    目的 制定科学合理的血液复检规则并对其进行评价.方法 以国际血液学41条复检规则为参考,结合Beckman-coulter AC.T 5diff血液分析仪的测试性能及报警系统,自定复检规则.对1 400例门诊和住院病人的标本进行全自动血细胞分析并同时涂片镜检,观察细胞数量和形态学变化.比较二者结果,对各条自定复检规则进行统计学分析.结果各条规则中假阳性率最高的是异常淋巴细胞,其次是巨大不成熟细胞及单核细胞.真阳性率最高的是粒细胞相对计数.自定复栓规则形态学评估真阳性率12.3%,假阳性率2.7%,真阴性率83.0%,假阴性率2.0%.结论 制定适合Beck-man-coulter AC.T 5diff血液分析仪的15条复检规则,照此执行,既可保证工作质量又提高工作效率,为临床提供更具参考价值的信息.%Objective To establish the microscope review criteria for the Beckman-coulter AC. T 5diff hematology analyzer. Methods Totally 1 400 blood samples were tested by using the Beckman-coulter AC. T 5diff hematology analyzer and smeared for microscopic examination. True positive rate.false positive rate.true negative rate and false negative rate were e-valuated and calculated according to the 41 rules suggested by the International Consensus Group for hematology review as well as the particular alarm system of the Beckman-coulter AC. T 5diff hematology analyzer. Results The most cases showing false positive rate were abnormal lymphocytes, large immature cells and monocytes. The most case showing true positive rate was relative granulocyte count. Microscopic examination results:true positive rate,false positive rate.true negative rate and false negative rate were respectively 12.3%,2. 7%,83. 0% and 2. 0%, respectively. Conclusion Establishment of reasonable microscope review criteria is beneficial to improving work efficiency, reducing probability of missed diagnosis and providing more useful information for clinical.

  2. Microchip Coulter particle counter

    DEFF Research Database (Denmark)

    Larsen, Ulrik Darling; Blankenstein, Gert; Branebjerg, J.


    This paper presents a micro device employing the Coulter principle for counting and sizing of living cells and particles in liquid suspension. The microchip Coulter particle counter (μCPC) has been employed in a planar silicon structure covered with glass, which enables detailed observation during...

  3. Micropipette as Coulter counter for submicron particles (United States)

    Rudzevich, Yauheni; Ordonez, Tony; Evans, Grant; Chow, Lee


    Coulter counter based on micropipette has been around for several decades. Typical commercial Coulter counter has a pore size of 20 μ m, and is designed to detect micron-size blood cells. In recent years, there are a lot of interests in using nanometer pore size Coulter counter to detect single molecule and to sequence DNA. Here we describe a simple nanoparticle counter based on pulled micropipettes with a diameter of 50 -- 500 nm. Borosilicate micropipettes with an initial outer diameter of 1.00 mm and inner diameter of 0.5 mm are used. After pulling, the micropipettes are fire polished and ultrasound cleaned. Chlorinated Ag/AgCl electrodes and 0.1 M of KCl solution are used. The ionic currents are measured using an Axopatch 200B amplifier in the voltage-clamp mode. Several types and sizes of nanoparticles are measured, including plain silica and polystyrene nanospheres. The results will be discussed in terms of pH values of the solution and concentrations of the nanoparticles. Financial support from National Science Foundation (NSF-0901361) is acknowledged.

  4. Sensor and control for consistent seed drill coulter depth

    DEFF Research Database (Denmark)

    Kirkegaard Nielsen, Søren; Nørremark, Michael; Green, Ole

    The even placement of seeds at the correct depth is crucial for achieving the optimum yield. The depth of drill coulters on state-of-the-art seeding machines is normally set manually by downforce springs or weights will therefore react to different soil resistances. A prototype seeder with one...... drill coulter was constructed and tested in a rotational soil bin. The research concluded a potential of minimizing the low frequent drill coulter depth variations and a solution for providing an even coulter depth in soil....

  5. High-Speed Multipass Coulter Counter with Ultrahigh Resolution. (United States)

    Edwards, Martin A; German, Sean R; Dick, Jeffrey E; Bard, Allen J; White, Henry S


    Coulter counters measure the size of particles in solution by passing them through an orifice and measuring a resistive pulse, i.e., a drop in the ionic current flowing between two electrodes placed on either side of the orifice. The magnitude of the pulse gives information on the size of the particle; however, resolution is limited by variability in the path of the translocation, due to the Brownian motion of the particle. We present a simple yet powerful modified Coulter counter that uses programmable data acquisition hardware to switch the voltage after sensing the resistive pulse of a nanoparticle passing through the orifice of a nanopipet. Switching the voltage reverses the direction of the driving force on the particle and, when this detect-switch cycle is repeated, allows us to pass an individual nanoparticle through the orifice thousands of times. By measuring individual particles more than 100 times per second we rapidly determine the distribution of the resistive pulses for each particle, which allows us to accurately determine the mean pulse amplitude and deliver considerably improved size resolution over a conventional Coulter counter. We show that single polystyrene nanoparticles can be shuttled back and forth and monitored for minutes, leading to a precisely determined mean blocking current equating to sub-angstrom size resolution. PMID:26549738

  6. The comparison between liquid scintillation quenching standard sets produced by Beckman Instruments Inc. and by Shanghai Institute of Nuclear Research

    International Nuclear Information System (INIS)

    The activity correction percent difference and coefficient of variation have been compared between two commercial liquid scintillation quenching standard sets, which were produced by Shanghai Institute of Nuclear Research (INR) of China and Beckman Instruments Inc. of USA, respectively. The self-corrected and inter-corrected percent differences ± standard deviation for 3H standard sets are -1.26 ± 1.66% (INR-self), 1.08 ± 1.96% (Beckman-self), -2.63 ± 1.52% (Beckman by INR) and 2.74 ± 1.67% (INR by Beckman), respectively. The data as above for 14C standard sets are -0.13 ± 1.17%, 0.07 ± 0.55%, 0.25 ± 0.93% and -0.30 ± 2.09%, respectively. The two manufactures' quenching standard sets are fit for routine correction in liquid scintillation counter

  7. The Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin.

    LENUS (Irish Health Repository)

    Byrne, Brendan


    Commercially available prolactin immunoassays detect macroprolactin to variable degrees. Best practice requires laboratories to assess the cross-reactivity of their prolactin assay with macroprolactin, and where appropriate, introduce a screen for the presence of macroprolactin. Our policy has been to reanalyse hyperprolactinaemic samples following polyethylene glycol (PEG) precipitation and to report the resultant value as the monomeric prolactin content of the sample. The goal of this study was to determine the need to continue PEG precipitation when prolactin measurements with the Wallac AutoDELFIA were replaced by the Beckman DxI 800.

  8. Coulter dispersant as positive electrolyte additive for the vanadium redox flow battery

    International Nuclear Information System (INIS)

    Coulter dispersants were investigated as the additive into the positive electrolyte (more than 1.8 M vanadium ions) of vanadium redox flow battery (VRB). The electrolyte stability tests showed that, at 45, 50 and 60 °C, the addition of 0.050–0.10 w/w Coulter dispersant IIIA (mainly containing coconut oil amine adduct with 15 ethylene oxide groups) into the positive electrolyte of VRB could significantly delay the time of precipitate formation from 1.8–12.3 h to 30.3 h ∼ 19.3 days. Moreover, the trace amount of Coulter dispersant IIIA as the additive can enhance the electrolyte stability without changing the valence state of vanadium ions, reducing the reversibility of the redox reactions and incurring other side reactions at the electrode. Using the Coulter IIIA dispersant as the additive also improved the energy efficiency of the VRB. The UV–vis spectra confirmed that the trace amount of Coulter IIIA dispersant did not chemically react with V(V) to form new substances. The synergy of Coulombic repulsion and steric hindrance between the macromolecular cationic surfactant additive and the solution reduced the aggregation of vanadium ions into V2O5 and increased the supersaturation of V2O5 crystal in the solution.

  9. Comparison of Coulter volumes with radiometrically determined intracellular water volumes for cultured cells

    Energy Technology Data Exchange (ETDEWEB)

    Burres, N.S.; Cass, C.E.


    During methotrexate-induced differentiation of cultured human choriocarcinoma (BeWo) cells, proliferation is inhibited, morphologic and biochemical changes occur, and giant, often multinucleated, cells form. We have used the increase in cell volume as a marker of the mature syncytiotrophoblastlike phenotype. Uninduced and differentiated BeWo cells are not spherical, and theoretical considerations suggested that deviations in shape could result in significant errors in Coulter volume. To determine if the values obtained by electrical pulse sizing reflected the actual mass of BeWo cells, we have evaluated the relationship between Coulter volumes and intracellular water volumes obtained using a shape-independent estimate for eight cell types. A close correlation (r2 = 0.97) was found, indicating that cell volume changes in populations of irregularly shaped cells can be accurately measured using a Coulter instrument.

  10. Conformal transformation for the Coulter--Weinberg form of the equations for mass zero spin-2 field

    Energy Technology Data Exchange (ETDEWEB)

    Galles, C.D.; Zandron, O.S.


    In this work we show that it is impossible to introduce a third-rank tensor potential that preserves the conformal covariance of the mass zero spin-2 field equations in the Coulter--Weinberg scheme. (AIP)

  11. Capacitive Coulter counting: detection of metal wear particles in lubricant using a microfluidic device

    International Nuclear Information System (INIS)

    A microfluidic device based on the capacitance Coulter counting principle to detect metal debris particles in lubricant oil is presented. The device scans each individual metal debris particle as they pass through a microfluidic channel by monitoring the capacitance change. We first proved the feasibility of using the capacitance Coulter counting principle for detecting metal particles in a fluidic channel. Next, we tested the microfluidic device with aluminum abrasive particles ranging from 10 to 25 µm; the testing results show the microfluidic device is capable of detecting metal wear particles in low-conductive lubricant oil. The design concept demonstrated here can be extended to a device with multiple microchannels for rapid detection of metal wear particles in a large volume of lubricant oil. (technical note)

  12. Measuring selectivity of feeding by estuarine copepods using image analysis combined with microscopic and Coulter counting


    Tackx, M.L.M.; Zhu, L.; De Coster, W.; Billones, R.G.; Daro, M.H.


    Although estuarine zooplankters are generally believed to be detritivorous, high clearance rates by the estuarine copepods Eurytemora affinis and Acartia tonsa on natural estuarine microplankton have been reported in the literature. In order to enable detection of possible selectivity for these microplankton organisms over detritus, a method that measures clearance rates on total particulate matter is proposed. Image analysis is used to measure copepod gut contents, and combined with Coulter ...

  13. CMOS-Compatible Silicon-Nanowire-Based Coulter Counter for Cell Enumeration. (United States)

    Chen, Yu; Guo, Jinhong; Muhammad, Hamidullah; Kang, Yuejun; Ary, Sunil K


    A silicon-nanowire-based Coulter counter has been designed and fabricated for particle/cell enumeration. The silicon nanowire was fabricated in a fully complementary metal-oxide-semiconductor (CMOS)-compatible process and used as a field effect transistor (FET) device. The Coulter counter device worked on the principle of potential change detection introduced by the passing of microparticles/cells through a sensing channel. Device uniformity was confirmed by scanning electron microscopy and transmission electron microscopy. Current-voltage measurement showed the high sensitivity of the nanowire FET device to the surface potential change. The results revealed that the silicon-nanowire-based Coulter counter can differentiate polystyrene beads with diameters of 8 and 15 μm. Michigan Cancer Foundation-7 (MCF-7) cells have been successfully counted to validate the device. A fully CMOS-compatible fabrication process can help the device integration and facilitate the development of sensor arrays for high throughput application. With appropriate sample preparation steps, it is also possible to expand the work to applications such as rare-cells detection. PMID:26799578

  14. Label-free protein detection using a microfluidic Coulter-counter device

    DEFF Research Database (Denmark)

    Rodriguez-Trujíllo, Romén; Ajine, Mohammad Akram; Orzan, A.;


    A new method for measuring specific protein concentrations in solutions has been developed. The technique makes use of the Coulter effect for detecting and sizing of micro-scaled objects suspended in a buffer fluid. The method is completely label-free as it is only based on the electrical readout...... rat IgG in solution. When the analyte (rat IgG) is present oligomers of beads are formed. The electrical readout of the oligomers is different compared to a zero control sample with no rat IgG. Detection of the protein has been performed in a concentration as small as 14 ng/mL. The dynamic range of...

  15. Observation and analysis of the Coulter effect through carbon nanotube and graphene nanopores. (United States)

    Agrawal, Kumar Varoon; Drahushuk, Lee W; Strano, Michael S


    Carbon nanotubes (CNTs) and graphene are the rolled and flat analogues of graphitic carbon, respectively, with hexagonal crystalline lattices, and show exceptional molecular transport properties. The empirical study of a single isolated nanopore requires, as evidence, the observation of stochastic, telegraphic noise from a blocking molecule commensurate in size with the pore. This standard is used ubiquitously in patch clamp studies of single, isolated biological ion channels and a wide range of inorganic, synthetic nanopores. In this work, we show that observation and study of stochastic fluctuations for carbon nanopores, both CNTs and graphene-based, enable precision characterization of pore properties that is otherwise unattainable. In the case of voltage clamp measurements of long (0.5-1 mm) CNTs between 0.9 and 2.2 nm in diameter, Coulter blocking of cationic species reveals the complex structuring of the fluid phase for confined water in this diameter range. In the case of graphene, we have pioneered the study and the analysis of stochastic fluctuations in gas transport from a pressurized, graphene-covered micro-well compartment that reveal switching between different values of the membrane permeance attributed to chemical rearrangements of individual graphene pores. This analysis remains the only way to study such single isolated graphene nanopores under these realistic transport conditions of pore rearrangements, in keeping with the thesis of this work. In summary, observation and analysis of Coulter blocking or stochastic fluctuations of permeating flux is an invaluable tool to understand graphene and graphitic nanopores including CNTs. PMID:26712649

  16. Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry

    Directory of Open Access Journals (Sweden)

    Jennifer Pasquier


    Full Text Available The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp. The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading, we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.

  17. Laser supported particle detecting system in comparison to the Coulter Counter and the Hiac PA 720

    Energy Technology Data Exchange (ETDEWEB)

    Doell, B.; Hoffmann, E.; Lawler, D.


    The purpose of these investigations is to demonstrate the ability of a standard Laser-Doppler-Velocity-Equipment (LDV) to get not only information about local velocities and their fluctuations but also about local size and concentration of particles suspended in flowing medias. Such information are of main interest not only for fundamental research but also for particle (size) applications. Particle detecting systems are commonly used in various fields of interest, for example, in indicating the particular pollution of air, water or chemical agents. In water technologies, particle-analysing systems are especially applied to quantify the efficiency of processing units (e.g. filtration or sedimentation steps). Starting with the method of Chigier and co-workers (1979) some experiments were run to get similar information in fluid flow carrying particles. To test the quality of the laser results, simultaneous measurements were performed using standard particle analysing systems. The systems were the Coulter Counter and the Hiac PA 720. A detailed comparison of both was the object of an extra investigation (Lawler, 1984).

  18. Effects of particle's off-axis position, shape, orientation and entry position on resistance changes of micro Coulter counting devices

    International Nuclear Information System (INIS)

    With the recent advance in micro/nano-fabrication technology, micro Coulter counters have been widely used in detecting and characterizing micro- and nanoscale objects. In this paper, the electrical resistance change during translocation of a non-conducting particle through a channel is studied numerically. The numerical results are validated by proven analytical results available in the literature. The effects of particle's off-axis position, shape and orientation, and entry position are studied for particles with a large dynamic range. From the numerical results, a new fitted correlation is proposed that can accurately predict the resistance change caused by off-axis spherical particles regardless of their size. The shape and orientation effects of the electrical resistance change are studied by changing the axis ratio of spheroid particles and their orientation angles. Results show that a particle's shape and orientation have a significant influence on the resistance change. Simulation of an entry effect indicates that a particle starts to induce a resistance change before it enters the channel and still causes a resistance change even after the particle exits the channel completely. This study will offer some guidelines in designing and implementing Coulter counting devices and experiments, and provide insights into explaining experimental results

  19. Measurement of the volume growth rate of single budding yeast with the MOSFET-based microfluidic Coulter counter. (United States)

    Sun, Jiashu; Stowers, Chris C; Boczko, Erik M; Li, Deyu


    We report on measurements of the volume growth rate of ten individual budding yeast cells using a recently developed MOSFET-based microfluidic Coulter counter. The MOSFET-based microfluidic Coulter counter is very sensitive, provides signals that are immune from the baseline drift, and can work with cell culture media of complex composition. These desirable features allow us to directly measure the volume growth rate of single cells of Saccharomyces cerevisiae LYH3865 strain budding yeast in YNB culture media over a whole cell cycle. Results indicate that all budding yeast follow a sigmoid volume growth profile with reduced growth rates at the initial stage before the bud emerges and the final stage after the daughter gets mature. Analysis of the data indicates that even though all piecewise linear, Gomperitz, and Hill's function models can fit the global growth profile equally well, the data strongly support local exponential growth phenomenon. Accurate volume growth measurements are important for applications in systems biology where quantitative parameters are required for modeling and simulation. PMID:20717618

  20. 应用NCCLS文件对Beckman CX9全自动生化分析仪的精密度进行研究

    Institute of Scientific and Technical Information of China (English)



    目的对已使用了5年的Beckman CX9全自动生化仪进行精密度评价.方法通过测定K+、ALT、ALB、TG的含量,评价其批内精密度(Swr)、总精密度(ST).结果K+、ALT、ALB、TG高低浓度的Swr分别为0.23mmol/L、0.10mmol/L;1.82IU/L、0.90IU/L;1.94g/L、1.31g/;0.06mmol/L、0.02mmol/L;Sr分别为0.26 mol/L、0.12 mmol/L;1.62 IU/L、1.28 IU/L;1.77 g/L、1.23 g/L;0.08 mmol/L、0.03mmol/L;x2检验x2<x2(95%)P>0.05.结论该仪器使用5年后精密度依然良好.

  1. 免耕播种机圆盘破茬刀工作性能分析与试验%Analysis and experiment on working performance of disc coulter for no-tillage seeder

    Institute of Scientific and Technical Information of China (English)

    白晓虎; 林静; 吕长义; 胡艳清


    soil disturbance. Among them, rolling types of disc stubble-cutting coulters can easily cut through stubble under the soil surface without throwing stubbles out of the soil, so it is suitable for application in Northeast China. There are few studies on cutting velocity and working resistance of dis coulters. However, an understanding of cutting velocity and working resistance of dis coulters is necessary for design and application of dis coulters. Therefore, this study investigated the cutting velocity under the condition that coulters slides occurred, and working resistance in vertical direction as well as tractive resistance by kinematics and force analysis, the influence of auxiliary weight and unit working speed on stubble-cutting depth of three disc coulters (plain, wavy, and turbo) in single factor field experiments, and the influence of disc coulter type, auxiliary weight and unit working speed on tractor resistance in an orthogonal field experiment of three factors and three levels. Both theoretical analysis and field tests showed that the coulter slippage had negative effect on the cutting velocity during the stubble-cutting process, which suggested reducing the coulter slippage to the greatest extent in the application of dis coulters. The tractor resistance of coulters changed slightly during the process of cutting soil in an increasing and then decreasing trend during the process of stubble cutting. The cutting depth of the three coulters increased with increasing auxiliary weight. When the auxiliary weight was less than 48 kg, the cutting stubble depth of plain coulter was largest, followed by the turbo coulter as well as the wave coulter. In practice, stubble under the ground must be totally cut through in order to make sure that no-tillage planter could be in good operation. To obtain a cutting depth of 80 mm or above, the minimum auxiliary weight of the plain, wavy and turbo disc coulter was required to be 48, 72 and 60 kg respectively. Since the total

  2. Enough room for Williams and IMF? / Paul Beckman

    Index Scriptorium Estoniae

    Beckman, Paul


    Lõppesid Leedu ja USA energeetikakompanii Williams International läbirääkimised Leedu naftakompleksis osaluse omandamise asjus. IMF uurib Leedu majanduslikku arengut, mida tehing Williamsiga komplitseerib

  3. Analytical validation of serum bone alkaline phosphatase (BAP OSTASE) on Liaison


    Cavalier, Etienne; Rozet, Eric; Carlisi, Agnès; Bekaert, Anne-Catherine; ROUSSELLE, Olivier; Hubert, Philippe; Chapelle, Jean-Paul; Delanaye, Pierre


    Background: The goal of this study was to validate the DiaSorin Liaison BAP OSTASE, a new method for measurement of bone alkaline phosphatase (BAP), and to compare this method with the Beckman-Coulter Access Ostase. We also wanted to establish the reference range for BAP in adults and children. Methods: We determined the precision, functional sensitivity, recovery, linearity and measurement uncertainty, accuracy profile and β-expectation limits. We defined an adult reference interval using i...

  4. Força de tração e volume de solo mobilizado por haste sulcadora em semeadura direta sobre campo nativo, em função do teor de água no solo, profundidade e velocidade de operação Draft and soil loosening by knife type coulter related to soil moisture and planter's working speed and depth

    Directory of Open Access Journals (Sweden)

    Carla T. C. Cepik


    's coulters. The specific aims of the present work are to determine the knowledge of coulter draft requirements and the amount of displaced soil and tractor slippage on a Paleudult soil at different soil moisture condition, in direct planting on natural pastures chemically killed. Draft requirements increased with an increase in working depth. The increase of the ground speed had no effect on draft when soil was dry or moist; it increased however, on friable soil. Soil displacement was always higher with dry soil and at 12 cm working depth. When soil was friable or dry, the front wheels traction was needed to keep tractor slippage within acceptable levels.


    Directory of Open Access Journals (Sweden)

    I. V. Kudryavtsev


    Full Text Available The article deals with applications of six-color flow analysis for studying the immune state parameters by means of flow cytometry. Whole blood from healthy donors was stained with combination of monoclonal antibodies, i.e., HLA-DR-FITC, CD16+56-PE, CD4-ECD, CD19-ECD, CD8-PC5.5, CD3-PC7, CD45-APC (Beckman Coulter, USA using a “no-wash” technology. To adjust the photomultiplier (PMT voltage, we used the tubes with each of the tested monoclonal antibodies, PMT voltage was considered optimal when the negative population was located in the middle of the first decade at the logarithmic scale. The compensatory adjustment was performed in automatic mode, using Navios software (Beckman Coulter, USA. We discuss an optimal gating strategy in order to assess the populations of interest: total T cells (CD3+CD19-, T helper cells (CD3+CD4+, cytotoxic T lymphocytes (CD3+CD8+, B cells (CD3-CD19+, NK cells (CD3-CD16+CD56+, NKT cells (CD3+CD16+CD56+, activated T lymphocytes (CD3+HLA-DR+. 

  6. Decreasing Quality of the New Generations of Anti-Müllerian Hormone Assays

    Directory of Open Access Journals (Sweden)

    Krzysztof Lukaszuk


    Full Text Available Anti-Müllerian hormone (AMH measurements are widely used to optimize the stimulation protocols. First generation AMH kits correlated well with ovarian reserve and response to stimulation. In the present study we aimed to asses if the new generation kits share the same accurate correlations. Retrospective data were collected from 8323 blood samples. For comparison we used Immunotech I generation kit (ImI 4035 samples, Beckman Coulter II generation kit RUO (BCII RUO 3449, samples and Beckman Coulter II generation kit with IVD certificate (BCII IVD 839 samples. We compared average AMH concentrations measured with different kits, as well as correlation between kits. We also compared average AMH concentrations in sera collected on different cycle days and samples of different quality of preservation. AMH serum concentrations differed for each kit, ranging 4.4 ± 4.12 (mean ± SD for the ImI, 2.68 ± 3.15 for the BCII RUO, and 1.64 ± 2.85 for BCII IVD. The mean differences from an adjusted regression model were −48.7%, −40%, and −69.2%, respectively. In conclusion, the changes of the BC AMH kits are unpredictable; however, the improvement of them is still possible. It would be very dangerous to use elaborated stimulation protocol (based on the Ist generation AMH results with the results from the IInd generation assays.

  7. Determination of cell volume during equilibrium freezing process

    Institute of Scientific and Technical Information of China (English)

    ZHAO Gang; GAO Dayong; HE Liqun; WANG Peitao; DING Weiping; XIE Xiaojian; LIU Zhong; ZHANG Haifeng; SHU Zhiquan; LUO Dawei


    A new type electronic particle counter (EPC, MultisizerTM 3, Beckman Coulter Inc., USA) was used to determine the volumes of human red blood cells (RBCs) in NaCl solutions of different osmolalities. The thermodynamics model describing cell response during freezing process was used to simulate the volume change of RBC in 0.9% NaCl solution during equilibrium freezing process. It was assumed that the effect of temperature on cell volume can be neglected compared to that of osmolality, then by using the phase diagram for the binary system sodium chloride/water, the osmolalities of the NaCl solution under different sub-zero temperatures can be obtained (converted from mass concentration), then the calculated values of RBC volumes can be validated by the experiments.

  8. Comparison study of trapped water in human erythrocytes by EPC and DSC method and its application

    Institute of Scientific and Technical Information of China (English)

    ZHAO; Gang; HE; Liqun; GUO; Xiaojie; LIU; Zhong; LUO; Dawei


    The novel differential scanning calorimetry method for determining trapped water volume of human red blood cell during freezing process has been reexamined. Results show that the final erythrocyte volume is 53% of its isotonic volume after freezing to -40℃. An electronic particle counter (MultisizerTM III, Beckman Coulter Inc., USA) was used to measure cell volume changes in response to hypertonic solution. Using this approach, when extracellular solution was 3186 mOsm, the equilibrium cell volume was found to be 57% of its isotonic value. Both results indicate that 34%-40% of intracellular water is trapped and cannot respond to osmotic difference between intra- and extracellular solution. These findings are consistent with the published data: at least 20%-32% of the isotonic cell water volume is retained within RBCs during freezing. Some applications of the values of trapped water are addressed.

  9. POCT法和常规检测法在BNP检测中的对比分析%Comparative analysis of POCT and routine test in BNP testing

    Institute of Scientific and Technical Information of China (English)

    朱小飞; 季明德; 李思洋; 葛亮; 顾万建


    Objective To analyze the relevance between the point-of-care testing (POCT) and routine test in BNP testing .Meth-ods The whole blood samples or plasma samples from 40 inpatients were detected brain natriuretic peptide (BNP) by the Alere Triage® MeterPro fluorescence immunoassay analyzer (POCT ) or the Beckman Coulter Access ®2 chemiluminescence analyzer (routine test) ,respectively .The acquired data were subjected to comparative analysis according to the CLCS EP 9-A2 .Results The linear regression of the BNP content in the blood samples detected by POCT and the routine test was good ,the correlation coeffi-cient(r) was 0 .999 7 .Conclusion POCT and the routine test have good correlation in BNP testing .POCT for BNP testing has higher reliability and is applicable for clinical detection .%目的:比较即时检验(POCT )法和常规检测法在B型钠脲肽(BNP)检测中的相关性。方法运用 Alere Triage®MeterPro(美国)荧光免疫分析仪或Beckman Coulter Access®2化学发光仪分别检测40份住院患者全血或血浆样本中BNP含量,按照CLCS EP9-A2文件要求进行对比分析。结果 POCT法和常规检测法检测住院患者血样BNP含量的线性回归良好,相关系数(r)=0.9997。结论 POCT法和常规检测法在BNP检测中的相关性良好,POCT 法具有较高的可靠性,可以用于临床检测。

  10. Thrombocytopenia model with minimal manipulation of blood cells allowing whole blood assessment of platelet function. (United States)

    Tiedemann Skipper, Mette; Rubak, Peter; Halfdan Larsen, Ole; Hvas, Anne-Mette


    In vitro models of thrombocytopenia are useful research tools. Previously published models have shortcomings altering properties of platelets and other blood components. The aim of the present study was to develop a whole blood method to induce thrombocytopenia with minimal manipulation, and to describe platelet function in induced thrombocytopenia in individuals with healthy platelets. Hirudin anticoagulated blood was obtained from 20 healthy volunteers. One part of the blood was gently centrifuged at 130g for 15 minutes. The platelet-rich plasma was replaced with phosphate-buffered saline to establish thrombocytopenia. Various levels of thrombocytopenia were achieved by combining different volumes of baseline whole blood and thrombocytopenic blood. Platelet counts were measured by flow cytometry (Navios, Beckman Coulter) and routine haematological analyser (Sysmex XE-5000). Platelet function was analysed by impedance aggregometry (Multiplate® Analyzer, Roche) and by flow cytometry (Navios, Beckman Coulter) using collagen, adenosine diphosphate, thrombin receptor activating peptide-6 and ristocetin as agonists. Median baseline platelet count was 227×10(9)/l. The in vitro model yielded median platelet counts at 51×10(9)/l (range 26-93×10(9)/l). We observed minor, yet significant, changes in platelet size and maturity from baseline to modelled thrombocytopenia. In the thrombocytopenic samples, significant and positive linear associations were found between platelet count and platelet aggregation across all agonists (all p-valuesblood-based model of thrombocytopenia was established and validated. This new model serves as a useful future tool, particularly to explore platelet function in patients with thrombocytopenia. PMID:26555800

  11. Successfully accelerating translational research at an academic medical center: The University of Michigan-Coulter Translational Research Partnership Program.


    Kenneth J Pienta


    Translational research encompasses the effective movement of new knowledge and discoveries into new approaches for prevention, diagnosis, and treatment of disease. There are many roadblocks to successful bench to bedside research, but few have received as much recent attention as the “valley of death”. The valley of death refers to the lack of funding and support for research that moves basic science discoveries into diagnostics, devices, and treatments in humans, and is ascribed to be the re...

  12. Lymphocytes sensitivity to Fas stimulation in healthy and asthmatic children.

    Directory of Open Access Journals (Sweden)

    Katarzyna Gomułka


    Full Text Available The T cell hypothesis of asthma is based on the concept that the disease is driven and maintained by the persistence of a specialized subset of chronically activated T memory cells sensitized against an array of allergenic, occupational or viral antigens. Overreaction of CD4+ T cells in the peripheral blood and airway tissues is an invariant feature of asthma; therefore a potent mechanism for augmenting the number of activated T cells in this disease would be the resistance to the normally programmed pathway for cell death. The aim of the study was to evaluate the presence of apoptotic markers on peripheral blood lymphocytes from healthy and asthmatic children before and after stimulation with antiCD95 antibodies. The blood was collected from 21 children with atopic asthma suffering from allergic rhinitis because of house dust mite and/or grass pollen allergens and 8 healthy children matched for their age and sex. Blood was mixed with purified monoclonal antibody antiCD95 (Beckman Coulter, incubated for 24 hours and than stained with Annexin V andPI (Becton Dickinson. Prepared suspensions were analyzed with Cytomics FC 500 (Beckman Coulter flow cytometer. Annexin V(+/PI(- cells were characterized as early apoptotic, Annexin V(+/PI(+ as late apoptotic and Annexin V(-/PI(+ as dead. In unstimulated sample from asthmatic children 21.09+/-11.20% cells were characterized as Annexin V positive/PI negative. After stimulation with antiCD95 Annexin V positive/PI negative cells constituted 18.72+/-9.42% of cells, p=0.1. In unstimulated sample from healthy children 11.69+/-6.70% cells were characterized as Annexin V positive/PI negative. In the sample stimulated with antiCD95 16.54+/-2.98% of cells were Annexin V positive/PI negative, p=0.02. There were no differences between results of late apoptotic and necrotic lymphocytes from healthy and asthmatic children. Performed research indicates that lymphocytes from asthmatic children are resistant to Fas

  13. Pilot-Scale Investigation of Liquid Aluminum Filtration through Ceramic Foam Filters: Comparison between Coulter Counter Measurements and Metallographic Analysis of Spent Filters


    Duval, Hervé; Rivière, Carlos; Laé, Émilie; Le Brun, Pierre; Guillot, Jean-Bernard


    A pilot has been designed to study the mechanisms associated with liquid aluminum filtrationthrough ceramic foam filters (CFFs). Before entering the filtering bowl, the liquid metal isseeded with massive artificial inclusions. A theoretical analysis of the filtration of massiveinclusions shows that there are two preponderant deposition mechanisms, i.e., direct interceptionand sedimentation, which explain qualitatively the effect of the operating conditions on themeasured filtration efficiency...


    Institute of Scientific and Technical Information of China (English)

    左建新; 任立盛; 邵翠华; 刘美新; 王言奎


    目的 通过血清抗苗勒管激素(AMH)的测定,评价双侧子宫动脉栓塞术对卵巢储备功能的影响.方法 采用Beckman Coulter ELISA法检测20例宫颈妊娠病人双侧子宫动脉栓塞术前及术后1、6个月血清AMH水平,并比较其变化.结果 宫颈妊娠病人子宫动脉栓塞术前至术后1、6个月血清AMH水平分别为(7.99±4.15)、(7.11±3.02)和(8.33±4.87)mg/L,手术前后比较差异无显著性(F=0.93,P>0.05).结论 子宫动脉栓塞术治疗宫颈妊娠不影响卵巢储备功能.

  15. Ultrasonic sonoelastography of scrotum in the diagnosis of male fertility

    Directory of Open Access Journals (Sweden)

    O. B. Zhukov


    Full Text Available Objective: to determine the possibility of using detect the scrotum in assessing reproductive function of men.Materials and methods. The study was conducted in men aged 20–44 years. The first group of men was 25 patients with secretory infertility. The second group included 25 male infertility varicocele. The control group presents 12 men in the age range 22–31 year with the implementation of the reproductive function of 1 or more children. The study was performed by ultrasonic scanner expert class Acuson S2000 manufacture of the company Siemens, was used microwave sensor 18L6 HD. Sex hormones were studied on immunochemiluminescence analyzer Access 2 production Beckman Coulter (USA. Obtaining and study of ejaculate was carried out according to Recommendations of the WHO (5th edition.Results. The application of research shear transverse wave Vs men with secretory infertility and varicocele is accompanied by the increase of its value to 1.5–1.6 m/s and is characterized by 35–45 % from similar parameters of reproductive healthy men. In half of the cases studies such an increase in performance and decrease in the area of mapping the so-called “soft” areas of the epididymis is accompanied by a decrease in the concentration and actively motile sperm. The use of such method of instrumental diagnostics will allow at the stage of preliminary ultrasound screening to suspect a violation of the reproductive capacity of men.

  16. An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems

    Directory of Open Access Journals (Sweden)

    Verschraegen Gerda


    Full Text Available Abstract Background Currently, most laboratories identify yeasts routinely on the basis of morphology and biochemical reactivity. This approach has quite often limited discriminatory power and may require long incubation periods. Due to the increase of fungal infections and due to specific antifungal resistence patterns for different species, accurate and rapid identification has become more important. Several molecular techniques have been described for fast and reliable identification of yeast isolates, but interlaboratory exchangeability of identification schemes of molecular techniques has hardly been studied. Here, we compared amplified ITS2 fragment length determination by an ABI Prism 310 (Applied Biosystems, Foster City, Ca. capillary electrophoresis system with that obtained by a CEQ8000 (Beckman Coulter, Fullerton, Ca. capillary electrophoresis system. Results Although ITS2 size estimations on both systems differed and separate libraries had to be constructed for each system, both approaches had the same discriminatory power with regard to the 44 reference strains, identical identifications were obtained for 39/ 40 clinical isolates in both laboratories and strains from 51 samples were correctly identified using CEQ8000, when compared to phenotypic identification. Conclusion Identification of yeasts with ITS2-PCR followed by fragment analysis can be carried out on different capillary electrophoresis systems with comparable discriminatory power.

  17. Normal lymphocyte immunophenotype in an elderly population

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    Sâmia Macedo Queiroz Mota Castellão Tavares


    Full Text Available OBJECTIVE: The aim of this work was to evaluate the lymphocyte immunophenotype in an elderly population.METHODS: This study enrolled 35 over 60-year-old volunteers and a control group composed of 35 young adults. The study included elderly without diseases that might affect the functioning of the immune system. These individuals were consulted by doctors and after a physical examination, laboratory tests were performed using a Beckman Coulter (r flow cytometer. The GraphPad Prism computer program was employed for statistical analysis with the level of significance being set for p-values <0.05.RESULTS: There is a statistically significant reduction in the number of lymphocytes (CD8 +, CD2 + and CD3 + cells in the elderly compared to young adults. These low rates are explained by changes attributed to aging and may be partly responsible for the reduction in the cellular immune response, lower proliferative activity and the low cytotoxicity of lymphocytes.CONCLUSION: These parameters showed greater impairment of adaptive immunity in the elderly population and can therefore explain the greater fragility of the aged body to developing diseases.

  18. Apoptosis induced by (di-isopropyloxyphoryl-Trp)2-Lys-OCH3 in K562 and HeLa cells

    Indian Academy of Sciences (India)

    Feng Liu; Shi-Ying Liu; Ping Xu; Zhen-Hua Xie; Guo-Ping Cai; Yu-Yang Jiang


    According to the method used in our laboratory, our group synthesized (DIPP-Trp)2-Lys-OCH3. It inhibited the proliferation of K562 and HeLa cells in a dose- and time-dependent manner with an IC50 of 15.12 and 42.23 M, respectively. (DIPP-Trp)2-Lys-OCH3 induced a dose-dependent increase of the G2/M cell population in K562 cells, and S cell population in HeLa cells; the sub-G0 population increased dramatically in both cell lines as seen by PI staining experiments using a FACS Calibur Flow cytometer (BeckmanCoulter, USA). Phosphatidylserine could significantly translocate to the surface of the membrane in (DIPP-Trp)2-Lys-OCH3-treated K562 and HeLa cells. The increase of an early apoptotic population was observed in a dose-dependent manner by both annexin-FITC and PI staining. It was concluded that (DIPP-Trp)2-Lys-OCH3 not only induced cells to enter into apoptosis, but also affected the progress of the cell cycle. It may have arrested the K562 and HeLa cells in the G2/M, S phases, respectively. The apoptotic pathway was pulsed at this point, resulting in the treated cells entering into programmed cell death. (DIPP-Trp)2-Lys-OCH3 is a potential anticancer drug that intervenes in the signalling pathway.

  19. Gluten determination by gliadin enzyme-linked immunosorbent assay kit: interlaboratory study. (United States)

    Gabrovská, Dana; Rysová, Jana; Filová, Vanda; Plicka, Jan; Cuhra, Petr; Kubík, Martin; Barsová, Sona


    An interlaboratory study with 10 participants was performed to obtain validation and performance data for an enzyme-linked immunosorbent assay (ELISA) kit developed for quantitative gluten determination in foods. The ELISA kit used for this study is based on 2 monoclonal and 1 polyclonal antibody developed by Immunotech, a Beckman Coulter Co. This kit did not show any false positive results or cross-reactivity with oat, rice, maize, and buckwheat. The gliadin standard from the Working Group on Prolamin Analysis and Toxicity was included in the kit as reference material for calibration. All participants obtained a gliadin ELISA kit with Standard Operational Procedure and a form for recording test results. The study included 13 samples labeled as "gluten-free" and 2 samples spiked by wheat flour. Seven samples had gliadin content below the limit of quantitation (LOQ) of the method, and 1 sample exceeded the highest calibration level. Gliadin content in the range from 10 to 157 mg/kg (1st day) and from 11 to 183 mg/kg (2nd day) was found in 7 samples (including 2 spiked samples). Results of these samples were used for further statistical analysis and evaluation. The Cochran, Dixon, and Mandel statistical tests were applied for detection of outliers. The LOQ of the kit was estimated. PMID:16512241

  20. Reliable and accurate CD4+ T cell count and percent by the portable flow cytometer CyFlow MiniPOC and "CD4 Easy Count Kit-Dry", as revealed by the comparison with the gold standard dual platform technology.

    Directory of Open Access Journals (Sweden)

    Milena Nasi

    Full Text Available An accurate and affordable CD4+ T cells count is an essential tool in the fight against HIV/AIDS. Flow cytometry (FCM is the "gold standard" for counting such cells, but this technique is expensive and requires sophisticated equipment, temperature-sensitive monoclonal antibodies (mAbs and trained personnel. The lack of access to technical support and quality assurance programs thus limits the use of FCM in resource-constrained countries. We have tested the accuracy, the precision and the carry-over contamination of Partec CyFlow MiniPOC, a portable and economically affordable flow cytometer designed for CD4+ count and percentage, used along with the "CD4% Count Kit-Dry".Venous blood from 59 adult HIV+ patients (age: 25-58 years; 43 males and 16 females was collected and stained with the "MiniPOC CD4% Count Kit-Dry". CD4+ count and percentage were then determined in triplicate by the CyFlow MiniPOC. In parallel, CD4 count was performed using mAbs and a CyFlow Counter, or by a dual platform system (from Beckman Coulter based upon Cytomic FC500 ("Cytostat tetrachrome kit" for mAbs and Coulter HmX Hematology Analyzer (for absolute cell count.The accuracy of CyFlow MiniPOC against Cytomic FC500 showed a correlation coefficient (CC of 0.98 and 0.97 for CD4+ count and percentage, respectively. The accuracy of CyFlow MiniPOC against CyFlow Counter showed a CC of 0.99 and 0.99 for CD4 T cell count and percentage, respectively. CyFlow MiniPOC showed an excellent repeatability: CD4+ cell count and percentage were analyzed on two instruments, with an intra-assay precision below ± 5% deviation. Finally, there was no carry-over contamination for samples at all CD4 values, regardless of their position in the sequence of analysis.The cost-effective CyFlow MiniPOC produces rapid, reliable and accurate results that are fully comparable with those from highly expensive dual platform systems.

  1. [Hematological indices of rats having complete and vitamin-deficient diets enriched with dietary fibers]. (United States)

    Mustafina, O K; Trushina, É N; Kosheleva, O V; Pereverzeva, O G; Kodentsova, V M; Vrzhesinskaia, O A


    The hematological indices in 48 rats Wistar male with initial body weight 58.1+/- 0.5g has been studied. The rats were divided into 6 group and fed the complete semi-synthetic diet, containing 100% or 20% of vitamin mixture (Vit) with or without addition of dietary fiber (DF) in the form of wheat bran (5% of diet mass) during 4 weeks. The animals of the 1 group received 100% of vitamin mixture (100% Vit); of the 2 group--100% Vit+DF; 3 group--20% of vitamin mixture (20% Vit); 4 group--20% of vitamin mixture and DF (20% Vit+DF). The next 5 days rats from vitamin-deficient groups were fed with diets supplemented with 80% of Vit: (5 group--20% Vit+ 80% Vit; 6 group--20% Vit+DF+80% Vit). The animals fed vitamin-deficient diet lag significantly in growth from animals fed a complete diet. The growth curve of rats fed a diet with DF took an intermediate position. The studies were carried out at the Hematology analyzer "Coulter AC TTM 5 diff OV" (Beckman Coulter, USA) under the program, developed for the study of rat blood. Mean corpuscular volume (MCV) and mean corpuscular hemoglobin content (MCHC) were significantly decreased in rats with combined vitamin deficiency (20% Vit) in the diet compared with those of control group rats, while the compensatory increase in the number of red blood cells (RBC) and leukocytopenia took place. The enrichment of vitamin-deficient diet with DF (20% Vit+DF) prevented MCV and RBC changes, but MCHC left reduced in comparison with the indicator of the rats in control group. Indicators characterizing the state of platelets had no statistically significant differences between the groups. Compensation of vitamin deficiency in the diet of rats from group 5 (20% Vit+80% Vit) led only to the normalization of MCV. After vitamin restore in the diet of group 6 (20% Vit+DF+80% Vit) all investigated parameters were indistinguishable from the parameters of the control group. This indicates a positive effect of wheat bran DF consumption on the studied

  2. Study on Profiling Mitochondria Proteome of Human Fetal Liver by Multi-dimensional Liquid Chromatography and Liquid Chromatography-Ion Trap Mass Spectrometry%多维液相色谱及液相色谱-离子阱质谱法研究人胎肝线粒体蛋白质组

    Institute of Scientific and Technical Information of China (English)

    张养军; 石蓉; 孟庆芳; 王京兰; 蔡耘; 朱云平; 贺福初; 钱小红


    In proteomics study, because there were biases of two-dimensional gel electrophoresis in molecular weights, pH and hydrophobicity, multi-dimensional protein identification technology (MuD-PIT)was proposed. Due to the core part of the technique being digestion of total proteins in the first step, some drawbacks appeared, such as complicating the separation of samples, dominating the influence of proteins with high abundance on the identification of proteins with low abundance and losing some information of proteins,for example, post-translational modification et al. , these resulted in the limitation of applications for the method. Taking into account of those factors mentioned above, when studying the mitochondria proteome of human fetal liver with great significance in life process, Proteome Lab PF2D system (Beckman Coulter, USA) was employed. The fractions of extract from fetal liver tissues by saccharose- density gradient centrifugation were prepared on a high velocity centrifuge. The desalting and solvent exchanging of the fractions with Start buffer (pH8. 5, Beckman Coulter, USA) were carried out on a PD-10(Sephadex G-25, 5 000Da) desalting column (amersham pharmacia biotech, Wikstroms,Sweden) according to the procedures described in its product instruction. Then the treated fractions were separated with chromatofocusing and reversed-phase liquid chromatography in tandem, respectively. After concentration and in-solution digestion of protein fractions,the peptide mixture was lyophilized, then resolved with 5% aqueous acytonitrile plus 0.1% formic acid, and finally analyzed with microLC-ESI-ion trap mass spectrometry(Thermo Finnigan, San Jose, CA, USA) and CapLC-ESI-QTOF mass spectrometry (Waters, USA). Mass spectrometric data was searched against IPI protein database with Thermo SEQUEST (Thermo Finnigan, San Jose, CA, USA) and Mascot. A primary database of mitochondrial proteome of human fetal liver was constructed, which could play central role in the large

  3. 多维液相色谱及液相色谱-离子阱质谱法研究人胎肝线粒体蛋白质组

    Institute of Scientific and Technical Information of China (English)

    张养军; 石蓉; 孟庆芳; 王京兰; 蔡耘; 朱云平; 贺福初; 钱小红


    In proteomics study, because there were biases of two-dimensional gel electrophoresis in molecular weights, pH and hydrophobicity, multi-dimensional protein identification technology (MuD-PIT)was proposed. Due to the core part of the technique being digestion of total proteins in the first step, some drawbacks appeared, such as complicating the separation of samples, dominating the influence of proteins with high abundance on the identification of proteins with low abundance and losing some information of proteins,for example, post-translational modification et al. , these resulted in the limitation of applications for the method. Taking into account of those factors mentioned above, when studying the mitochondria proteome of human fetal liver with great significance in life process, Proteome Lab PF2D system (Beckman Coulter, USA) was employed. The fractions of extract from fetal liver tissues by saccharose- density gradient centrifugation were prepared on a high velocity centrifuge. The desalting and solvent exchanging of the fractions with Start buffer (pH8. 5, Beckman Coulter, USA) were carried out on a PD-10(Sephadex G-25, 5 000Da) desalting column (amersham pharmacia biotech, Wikstroms,Sweden) according to the procedures described in its product instruction. Then the treated fractions were separated with chromatofocusing and reversed-phase liquid chromatography in tandem, respectively. After concentration and in-solution digestion of protein fractions,the peptide mixture was lyophilized, then resolved with 5% aqueous acytonitrile plus 0.1% formic acid, and finally analyzed with microLC-ESI-ion trap mass spectrometry(Thermo Finnigan, San Jose, CA, USA) and CapLC-ESI-QTOF mass spectrometry (Waters, USA). Mass spectrometric data was searched against IPI protein database with Thermo SEQUEST (Thermo Finnigan, San Jose, CA, USA) and Mascot. A primary database of mitochondrial proteome of human fetal liver was constructed, which could play central role in the large

  4. The routine leukocyte differential flow cytometry HematoFlow™ method: A new flagging system for automatic validation. (United States)

    Allou, Kaoutar; Vial, Jean-Philippe; Béné, Marie C; Lacombe, Francis


    The complete blood cell count and white blood cell differential are the first step in the biological diagnosis of hematological diseases. Both are currently performed by automated instruments which control data and produce alerts. If such flags are activated, the automated differential cannot be validated and the operator must activate a visual blood smear review. Microscopic examination is still today the reference method despite its lack of sensitivity and reproducibility. The HematoFlow™ (Beckman Coulter) system is the first flow cytometry commercialized method designed for the routine differential. Using six markers in five colors and an automated gating strategy, it provides differentials proven to be reliable for 17 leukocyte subpopulations detection. Relying first on a retrospective analysis of 6,462 blood samples processed by HematoFlow™, thresholds were determined to detect the presence of immature granulocytes and/or blast cells. All possible gating strategy misclassifications of leukocyte subpopulations were then summarized in a systematic nomenclature leading to the development of an original flag system based on the detection of aberrant localization of cell events in specific new bivariate histograms. Ultimately, more than 50% of the results could be automatically validated using the HematoFlow™ system, without any false negative, thereby dramatically contributing to an important decrease of technicians' workload. Moreover a noticeable help was given for smear review interpretation and new immunological flags led to the confirmation of blood disease after classical immunophenotyping. These results were confirmed in a second prospective study including 15,335 cases, where more than 50% of the results were automatically validated by this new flag system. MFC stands as being more and more essential for analyzing differentials in routine and this new flag system could greatly improve its implementation. PMID:25906976

  5. The Effects of Short-Term Intensive Exercise on Levels of Liver Enzymes and Serum Lipids in Kick Boxing Athletes

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    Ömer Kaynar


    Full Text Available Objective: In this study, it was aimed to evaluate the ef­fects of short-term intensive exercise on liver enzymes and serum lipid levels with kick boxing athletes. Methods: 23 voluntary athletes who were between the ages of 15-46 and who engaged in kick–boxing have tak­en place this study. Athletes were made to do 45 minutes of warming-up, breathing, and stretching and 50 minutes of technical and tactical practices and then they were made to do a training match, which is equal to a 2 min­utes 3 circuits (1 minute rest kick-box match. In venous blood samples which were taken from athletes before and after training, aspartate aminotransferase (AST, alanine aminotransferase (ALT, alkaline phosphatase (ALP and gamma glutamine transpeptidase (GGT, enzyme activity and total cholesterol, high-density lipoprotein-cholesterol (HDL-C, low density lipoprotein-cholesterol (LDL-C and triglycerides serum levels were analyzed via spectropho­tometric method in Beckman Coulter AU 5800 auto ana­lyzer. Body composition measurements of athletes were made with Tanita TBF 300 brand device, which works with bio-impedance analysis (BIA system. Results: As a result of our study, statistically increases in serum ALT, AST, ALP and GGT enzyme activities and in serum total cholesterol, HDL-C and LDL-C levels were detected following short-term intensive exercise, but no significant difference was observed in TG levels after in­tensive exercise. Conclusion: The blows to the abdomen during kickbox­ing sports competitions result in increased liver enzymes and increased serum lipids may occur to meet energy de­mand of the body during exercise.


    Directory of Open Access Journals (Sweden)

    Claudia Vlad


    Full Text Available Rheumatoid arthritis is a systemic inflammatory disease with still unknown aetiology. The purpose of our study was to comparatively investigate the level of haemoglobin, as well as of the heat shock proteins HSP60 and HSP70 as well as their specific antibodies serum levels in rheumatic patients, in order to evaluate their potential role as an aid in the diagnosis of this chronic pathology. This study was performed on patients with rheumatoid polyarthritis. The haemoglobin plasmatic concentration was assessed by a quantitative determination using the automatic analyzer Beckman Coulter Act5diff, while the thermal shock proteins HSP 60, HSP 70 as well as their respective serum antibodies were determined by Western blot method and ELISA assay, respectively. All the patients involved in this study exhibited low values of haemoglobin, known to be an important marker of haematological disorders. These results maintain the idea that anaemia, heart disease, osteoporosis are the most frequent complication for rheumatoid arthritis. The concept of overexpression of endogenous HSPs is central to hypotheses in which HSPs are implicated in the pathogenesis of autoimmune rheumatic disease. The quantification of HSPs levels in the serum of the rheumatic patients showed that both Hsp60 and Hsp70 levels are higher, especially in those patients who had as a secondary disease, like cardiac insufficiency and obesity. The HSP60 and HSP70 antibodies were also highly expressed in our patients. These lesions could be partially due to the fact that despite the ubiquitous and high homology of heat shock proteins among different species, they also represent important antigenic targets of the cellular and humoral immune response. Besides the low level of haemoglobin, the presence of a high level of heat shock proteins and of their corresponding antibodies may be considered as useful markers which could be correlated with the evolution and the severity of a long chain of

  7. White adipose tissue resilience to insulin deprivation and replacement.

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    Lilas Hadji

    Full Text Available Adipocyte size and body fat distribution are strongly linked to the metabolic complications of obesity. The aim of the present study was to test the plasticity of white adipose tissue in response to insulin deprivation and replacement. We have characterized the changes of adipose cell size repartition and gene expressions in type 1 diabetes Sprague-Dawley rats and type 1 diabetic supplemented with insulin.Using streptozotocin (STZ-induced diabetes, we induced rapid changes in rat adipose tissue weights to study the changes in the distribution of adipose cell sizes in retroperitoneal (rWAT, epididymal (eWAT and subcutaneous adipose tissues (scWAT. Adipose tissue weights of type 1 diabetic rats were then rapidly restored by insulin supplementation. Cell size distributions were analyzed using multisizer IV (Beckman Coulter. Cell size changes were correlated to transcriptional regulation of genes coding for proteins involved in lipid and glucose metabolisms and adipocytokines.The initial body weight of the rats was 465±5.2 g. Insulin privation was stopped when rats lost 100 g which induced reductions in fat mass of 68% for rWAT, 42% for eWAT and 59% for scWAT corresponding to decreased mode cell diameters by 31.1%, 20%, 25.3%, respectively. The most affected size distribution by insulin deprivation was observed in rWAT. The bimodal distribution of adipose cell sizes disappeared in response to insulin deprivation in rWAT and scWAT. The most important observation is that cell size distribution returned close to control values in response to insulin treatment. mRNAs coding for adiponectin, leptin and apelin were more stimulated in scWAT compared to other depots in diabetic plus insulin group.Fat depots have specific responses to insulin deprivation and supplementation. The results show that insulin is a major determinant of bimodal cell repartition in adipose tissues.

  8. Comparable flow cytometry data can be obtained with two types of instruments, Canto II, and Navios. A GEIL study. (United States)

    Solly, Françoise; Rigollet, Lauren; Baseggio, Lucille; Guy, Julien; Borgeot, Jessica; Guérin, Estelle; Debliquis, Agathe; Drenou, Bernard; Campos, Lydia; Lacombe, Francis; Béné, Marie C


    Flow cytometry (FC) instruments settings classically rely on local establishment of photomultipliers (PMT) voltages adapted to the measurements expected to be performed. In the era of multiparameter FC (MFC), it appears more and more desirable that comparable patterns of fluorescence are obtained in different settings. This relies on a harmonization of settings between instruments. Although this has been shown to be feasible within a given brand of flow cytometers, little information is available about broader comparisons in a given center or in a multicenter fashion. Here, we report a two-phase series of experiments first performed between a Canto II (BD Biosciences) and a Navios (Beckman Coulter) instruments in the same center. PMT values adjusted on the reference instrument (RI) Canto II were used to establish target values for PMT settings on the paired Navios practice instrument (PI). This allowed to show the good correlation of all but peaks 1 and 2 of Rainbow(®) beads between RI and PI. Using 4- or 8-color stained leukocytes, the similitude of the settings was further confirmed. A complex set of matrices was then established between five centers all equipped with both instruments. Using Bland & Altman difference comparisons for median fluorescence values, it was shown that using either Rainbow beads or CD16 stained polymorphonuclears to set-up target values on the RI CantoII, highly superimposable results could be obtained on all 9 PI. The latter were obtained using Rainbow beads or Compbeads(®) for comparisons. In summary, this two-phase study demonstrates the feasibility of different methods allowing for a robust harmonization of settings for MFC. PMID:24151234

  9. Diurnal Variations of Circulating Extracellular Vesicles Measured by Nano Flow Cytometry.

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    Kirsty M Danielson

    Full Text Available The identification of extracellular vesicles (EVs as intercellular conveyors of biological information has recently emerged as a novel paradigm in signaling, leading to the exploitation of EVs and their contents as biomarkers of various diseases. However, whether there are diurnal variations in the size, number, and tissue of origin of blood EVs is currently not known, and could have significant implications when using EVs as biomarkers for disease progression. Currently available technologies for the measurement of EV size and number are either time consuming, require specialized equipment, or lack sufficient accuracy across a range of EV sizes. Flow cytometry represents an attractive alternative to these methods; however, traditional flow cytometers are only capable of measuring particles down to 500 nm, which is significantly larger than the average and median sizes of plasma EVs. Utilizing a Beckman Coulter MoFlo XDP flow cytometer with NanoView module, we employed nanoscale flow cytometry (termed nanoFCM to examine the relative number and scatter distribution of plasma EVs at three different time points during the day in 6 healthy adults. Analysis of liposomes and plasma EVs proved that nanoFCM is capable of detecting biologically-relevant vesicles down to 100 nm in size. With this high resolution configuration, we observed variations in the relative size (FSC/SSC distributions and concentration (proportions of EVs in healthy adult plasma across the course of a day, suggesting that there are diurnal variations in the number and size distribution of circulating EV populations. The use of nanoFCM provides a valuable tool for the study of EVs in both health and disease; however, additional refinement of nanoscale flow cytometric methods is needed for use of these instruments for quantitative particle counting and sizing. Furthermore, larger scale studies are necessary to more clearly define the diurnal variations in circulating EVs, and thus

  10. Immune responses in the Japanese pufferfish (Takifugu rubripes) head kidney cells stimulated with particulate silica. (United States)

    Morimoto, Takashi; Biswas, Gouranga; Kono, Tomoya; Sakai, Masahiro; Hikima, Jun-Ichi


    Studies on immune response to crystal silica in mammals indicate immune stimulation effect of environmental parameters including silica or asbestos, but there is no information on this aspect in lower vertebrates. Therefore, we examined expression of cytokine genes related to innate immunity in the Japanese pufferfish, Fugu (Takifugu rubripes) head kidney (HK) cells stimulated with particulate silica at 10 and 50 μg mL(-1). Expression of eleven cytokine genes was analyzed by the multiplex RT-PCR method (GenomeLab Genetic Analysis System, GeXPS; Beckman Coulter Inc.). Additionally, to confirm functionality of activated inflammatory immunity, we assessed phagocytic activity. Expression of NLR family genes as potential sensor molecules of inflammasome and inflammasome-associated genes (ASC and caspase-1) was also confirmed in HK cells by quantitative real-time PCR (qRT-PCR). As a result, an increased gene expression of pro-inflammatory cytokines (IL-6, IL-17A/F3, TNF-α, TNF-β and IFN-γ) and other cytokines (IL-4/13A, IL-4/13B, Type I-IFN) was recorded in particulate silica stimulated HK cells. Moreover, phagocytic activity showed a tendency to significantly increase in stimulated monocyte of HK cells after 6 h. Expression of NLR-C9 and NLR-C12 genes significantly increased in silica-stimulated HK cells. The particulate silica also significantly induced expression of inflammosome-associated genes, which may relate to the induced NLR-Cs. PMID:26702561

  11. 血清补体C3和C4对慢性乙型肝炎患者肝组织病理状态的判断价值%Serum complement C3 and C4 levels for evaluation of hepatic pathological status in patients with chronic hepatitis B

    Institute of Scientific and Technical Information of China (English)

    张占卿; 陆伟; 王雁冰; 饶敏; 冯洁; 沈芳; 冯艳玲


    AIM: To explore the possibility of using serum complement C3 and C4 levels to estimate the hepatic pathological status in patients with chronic hepatitis B.METHODS: Four hundred and fifty-six patients with pathologically proven chronic hepatitis B were enrolled in present study. Serum complement C3 and C4 levels were determined using the Beckman-Coulter Immage 800 im-munochemistry system and auxiliary reagents (Beckman-Coulter Inc., USA). Bayes stepwise discriminant analysis was used for evaluation of serum complement C3 and C4 in discriminating hepatic pathological grade and stage.RESULTS: Only serum C3 or C4 entered the Fisher's discriminant functions for discriminating hepatic pathological grade and stage according to the entry criteria of the independents (Wilks' λ = 0.967, x2 = 15.312, P = 0.002 and Wilks' λ = 0.938, x2 = 28.724, P = 0.000). The correctly classified rates of the originally grouped cases by the discriminant functions built with serum complement C3 and C4 were 46.34% for G1, 6.28% for G2, 20.61% for G3, 72.73% for G4 and 6.90% for SO, 46.51% for S1, 7.58% for S2, 8.91% for S3, 61.11% for S4, respectively. According to the discriminant functions for discriminating different pathological grades and stages, patients had a higher probability of Gl and G4 when serum complement C3 level was no less than 0.84 g/L and no more than 0.65 g/L, respectively, and of SI and S4 when serum complement C4 level was no less than 0.18 g/L and no more than 0.14 g/L respectively.CONCLUSION: Serum complement C3 and C4 levels have appreciable significance in discriminating hepatic pathological grade and stage in patients with chronic hepatitis B.%目的:探讨血清补体C3 和C4判别慢性乙型肝炎患者肝组织病理学分级和分期的价值.方法:456 例经肝组织病理学检查的慢性乙型肝炎患者入选本研究.血清补体C3 和C4采用Beckman-Coulter Immage 800 免疫化学系统及其配套试剂测定.血清补体C3 和C4判别病理学分

  12. 血清补体C3和C4预测慢性乙型肝炎肝组织病理学状态的价值%Value of serum complement C3 and C4 for predicting hepatic pathological status in patients with chronic hepatitis B

    Institute of Scientific and Technical Information of China (English)

    张占卿; 陆伟; 饶敏; 王雁冰; 陈淑琴; 童海涛; 沈芳


    Objective To explore the clinical value of serum complement C3 and C4 for predicting the hepatic inflammatory activities and fibrotic levels in patients with chronic hepatitis B. Methods 416 patients with chronic hepatitis B with pathological diagnoses of liver tissue were enrolled in present study. Serum complement C3 and C4 were determined by Beckman-Coulter Immage 800 immunochemistry system and auxiliary reagents( Beckman-Coulter Inc. , USA). Results The areas under ROC curve of serun complement C3 and C4 for predicting significant inflammation( ≥G2 ) ,moderate inflammation( ≥G3 ),severe inflammation ( = G4 ) were respectively 0.602 (95% CI:0. 545-0. 659) and 0.550 (95 % CI:0.4890. 611 ), 0.620 ( 95% CI: 0. 559-0. 681 ) and 0. 606 ( 95% CI: 0.547-0. 666 ), 0. 803 ( 95% CI: 0. 6700. 937 ) and 0.654 (95 % CI:0. 486-0. 823 ); and those for predicting significant fibrosis ( ≥ S2 ), severe fibrosis( ≥S3 ) ,cirrhosis( = S4 ) were respectively 0. 590(95% CI:0. 531-0. 649) and 0. 582 (95% CI:0. 519-0. 644) ,0. 665 (95% CI:0. 612-0. 718) and 0. 655 (95% CI:0. 602-0. 707 ), 0. 669 (95% CI:0. 603-0. 736) and 0. 670(95% CI :0. 607-0. 734). The optimal cut-offs of serum complement C3 and C4 for predicting severe inflammation and cirrhosis were respectively ≤0.58 g/L and ≤0.12 g/L, and the corresponding sensitivity, specificity, accuracy were 0. 750 and 0. 480,0. 797 and 0. 792, 0. 796 and 0. 719. Conclusion Serum complement C3 and C4 have some value for predicting respectively hepatic severe inflammation and cirrhosis in patients with chronic hepatitis B.%目的 探讨血清补体C3和C4预测慢性乙型肝炎肝组织炎症活动度和纤维化程度的价值.方法 416例经肝组织病理学检查的慢性乙型肝炎患者入选本研究.血清补休C3和C4采用Beckman-Coulter Immage 800免疫化学系统及其配套试剂测定.结果 血清补体C3和C4预测显著炎症(≥G2)、中度炎症(≥G3)、重度炎症(=G4)

  13. Pseudoleukocytosis secondary to hepatitis C-associated cryoglobulinemia: a case report

    Directory of Open Access Journals (Sweden)

    Geara Abdallah


    Full Text Available Abstract Introduction Laboratory tests play a central role in assessing a patient and orienting the diagnostic evaluation. We report a case where the discrepancy between the manual and automatic cell count gave a hint to the final diagnosis. Case presentation A 55-year-old Caucasian man, known to have hepatitis C, was admitted with acute respiratory failure secondary to acute pulmonary edema and diffuse petechial rash of the lower extremities for the previous 2 months. The initial laboratory tests showed acute renal failure (creatinine of 2.6 mg/dL. During his hospital stay, the patient had a fluctuating white blood cell count with a recorded value of 96,000 cells/mL. On a peripheral smear, the blood cell count was in the normal range. The acute renal failure was secondary to membranoproliferative glomerulonephritis secondary to essential mixed cryoglobulinemia diagnosed by biopsy. The complete blood count values, performed by Beckman/Coulter GenS, were falsely high due to precipitation of plasma cryoglobulins at room temperature. This spurious leukocytosis was previously described in several case reports, but values as high as 96,000 cells/mL were never reported. Conclusion The presence of cryoglobulins in the blood creates a clinical challenge for the interpretation of several laboratory tests. Pseudoleukocytosis secondary to cryoglobulinemia has been observed in several reported cases with white blood cell counts up to 54,000 cells/mL at room temperature and 85,600 cells/mL at 4°C. If the cryoglobulin precipitates rapidly, aggregated cryoglobulin particles may be interpreted as blood cells. We report the first patient with pseudoleukocytosis secondary to hepatitis C cryoglobulinemia with a spurious leukocytosis of 96,000 cells/mL at room temperature. Other laboratory tests could also be affected: underestimation of true erythrocyte sedimentation rate, pseudothrombocytosis and pseudolymphocytosis. The precipitation can remove the hepatitis C

  14. Single Walled Carbon Nanohorns as Photothermal Cancer Agents

    Energy Technology Data Exchange (ETDEWEB)

    Whitney, John [Virginia Polytechnic Institute and State University (Virginia Tech); Sarkar, Saugata [Virginia Polytechnic Institute and State University (Virginia Tech); Zhang, Jianfei [Virginia Polytechnic Institute and State University (Virginia Tech); Do, Thao [Virginia Polytechnic Institute and State University (Virginia Tech); Manson, Mary kyle [Virginia Polytechnic Institute and State University (Virginia Tech); Campbell, Tom [Virginia Polytechnic Institute and State University (Virginia Tech); Puretzky, Alexander A [ORNL; Rouleau, Christopher M [ORNL; More, Karren Leslie [ORNL; Geohegan, David B [ORNL; Rylander, Christopher [Virginia Polytechnic Institute and State University (Virginia Tech); Dorn, Harry C [Virginia Polytechnic Institute and State University (Virginia Tech); Rylander, Nichole M [Virginia Polytechnic Institute and State University (Virginia Tech)


    Nanoparticles have significant potential as selective photo-absorbing agents for laser based cancer treatment. This study investigates the use of single walled carbon nanohorns (SWNHs) as thermal enhancers when excited by near infrared (NIR) light for tumor cell destruction. Absorption spectra of SWNHs in deionized water at concentrations of 0, 0.01, 0.025, 0.05, 0.085, and 0.1 mg/ml were measured using a spectrophotometer for the wavelength range of 200-1,400 nm. Mass attenuation coefficients were calculated using spectrophotometer transmittance data. Cell culture media containing 0, 0.01, 0.085, and 0.333 mg/ml SWNHs was laser irradiated at 1,064 nm wavelength with an irradiance of 40 W/cm{sup 2} for 0-5 minutes. Temperature elevations of these solutions during laser irradiation were measured with a thermocouple 8 mm away from the incident laser beam. Cell viability of murine kidney cancer cells (RENCA) was measured 24 hours following laser treatment with the previously mentioned laser parameters alone or with SWNHs. Cell viability as a function of radial position was determined qualitatively using trypan blue staining and bright field microscopy for samples exposed to heating durations of 2 and 6 minutes alone or with 0.085 mg/ml SWNHs. A Beckman Coulter Vi-Cell instrument quantified cell viability of samples treated with varying SWNH concentration (0, 0.01, 0.085, and 0.333 mg/ml) and heating durations of 0-6 minutes. Spectrophotometer measurements indicated inclusion of SWNHs increased light absorption and attenuation across all wavelengths. Utilizing SWNHs with laser irradiation increased temperature elevation compared to laser heating alone. Greater absorption and higher temperature elevations were observed with increasing SWNH concentration. No inherent toxicity was observed with SWNH inclusion. A more rapid and substantial viability decline was observed over time in samples exposed to SWNHs with laser treatment compared with samples experiencing laser

  15. Online analysis of europium and gadolinium species complexed or uncomplexed with humic acid by capillary electrophoresis-inductively coupled plasma mass spectrometry

    International Nuclear Information System (INIS)

    Detailed information on the geochemical behavior of radioactive and toxic metal ions under environmental conditions (in geological matrices and aquifer systems) is needed in order to assess the long-term safety of waste repositories. This includes knowledge of the mechanisms of relevant geochemical reactions, as well as associated thermodynamic and kinetic data. Several previous studies have shown that humic acid can play an important role in the immobilization or mobilization of metal ions due to complexation and colloid formation. In our project we investigate the complexation behavior of (purified Aldrich) humic acid and its influence on the migration of the lanthanides europium and gadolinium (homologs of the actinides americium and curium) in the ternary system consisting of these heavy metals, humic acid and kaolinite (KGa-1b) under almost natural conditions. Capillary electrophoresis (CE, Beckman Coulter P/ACE MDQ), with its excellent separation performance, was hyphenated with a homemade interface to inductively coupled plasma mass spectrometry (ICP-MS, VG Elemental PlasmaQuad 3) giving a system that is highly sensitive to the rare-earth element species of europium and gadolinium with humic acid. The humic acid used was also halogenated with iodine, which acted as an ICP-MS marker. To couple CE to ICP-MS, a fused silica CE capillary was flexibly fitted into a MicroMist 50 μl nebulizer with a Cinnabar cyclonic spray chamber in the external homemade interface. The chamber was chilled to a temperature of 4 C to optimize the sensitivity. 200 ppb of cesium were added to the CE separation buffer so that the capillary flow could be observed. A make-up fluid including 4 ppb Ho as an internal standard was combined with the flow from the capillary within the interface in order to get a fluid throughput high enough to maintain continuous nebulization. Very low detection limits were achieved: 125 ppt for 153Eu and 250 ppt for 158Gd. Using this optimized CE

  16. Evaluation of Oxacillin and Cefoxitin Disk and MIC Breakpoints for Prediction of Methicillin Resistance in Human and Veterinary Isolates of Staphylococcus intermedius Group. (United States)

    Wu, M T; Burnham, C-A D; Westblade, L F; Dien Bard, J; Lawhon, S D; Wallace, M A; Stanley, T; Burd, E; Hindler, J; Humphries, R M


    Staphylococcus pseudintermedius is a coagulase-positive species that colonizes the nares and anal mucosa of healthy dogs and cats. Human infections with S. pseudintermedius range in severity from bite wounds and rhinosinusitis to endocarditis; historically, these infections were thought to be uncommon, but new laboratory methods suggest that their true incidence is underreported. Oxacillin and cefoxitin disk and MIC tests were evaluated for the detection of mecA- or mecC-mediated methicillin resistance in 115 human and animal isolates of the Staphylococcus intermedius group (SIG), including 111 Staphylococcus pseudintermediusand 4 Staphylococcus delphini isolates, 37 of which were mecA positive. The disk and MIC breakpoints evaluated included the Clinical and Laboratory Standards Institute (CLSI) M100-S25 Staphylococcus aureus/Staphylococcus lugdunensis oxacillin MIC breakpoints and cefoxitin disk and MIC breakpoints, the CLSI M100-S25 coagulase-negative Staphylococcus (CoNS) oxacillin MIC breakpoint and cefoxitin disk breakpoint, the CLSI VET01-S2 S. pseudintermedius oxacillin MIC and disk breakpoints, and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) S. pseudintermedius cefoxitin disk breakpoint. The oxacillin results interpreted by the VET01-S2 (disk and MIC) and M100-S25 CoNS (MIC) breakpoints agreed with the results of mecA/mecC PCR for all isolates, with the exception of one false-resistant result (1.3% of mecA/mecC PCR-negative isolates). In contrast, cefoxitin tests performed poorly, ranging from 3 to 89% false susceptibility (very major errors) and 0 to 48% false resistance (major errors). BD Phoenix, bioMérieux Vitek 2, and Beckman Coulter MicroScan commercial automated susceptibility test panel oxacillin MIC results were also evaluated and demonstrated >95% categorical agreement with mecA/mecC PCR results if interpreted by using the M100-S25 CoNS breakpoint. The Alere penicillin-binding protein 2a test accurately detected all

  17. Research of complement activity in children with anaphylactoid purpura%儿童过敏性紫癜补体活性研究

    Institute of Scientific and Technical Information of China (English)

    肖珊珊; 刘丽晓; 张佳慧; 范慧子


    Objective To investigate the complement C3, C4 activity in children with anaphylactoid purpura of acute and convalescent phase. Methods 97 children with anaphylactoid purpura treated in our hospital from October 2009 to November 2011 were selected as research objects. According to the clinical manifestation, all patients were divided into the skin-type group, abdominal-type group, kidney-type group, joint-type group and mixed-type group. According to the progression disease, patients were divided into the acute phase and the convalescent phase. At the same time, the complement levels were compared with that 32 cases of healthy children, who were taken as the control group. Then the children' serum C3, C4 level were detected by immune scattering rate turbidimetric method using Array 360 scattering immune turbidimetric instrument produced in Beckman-coulter company. Results In the acute and convalescent phase of anaphylactoid purpura children, the serum C3 and C4 levels among different clinical type groups had no difference (P > 0.05). The serum C4 level in acute, convalescent phase groups and control group had no statistically significant difference (P > 0.05); but compared with the control group, the C3 level in acute phase reduced (P 0.05);急性期、恢复期与对照组相比,血清补体C4水平差异无统计学意义(P > 0.05);与对照组相比,急性期血清补体C3水平降低(P < 0.05),恢复期C3水平相对急性期升高,但仍较对照组低(P < 0.05).结论 不同临床分型过敏性紫癜患儿其血清补体水平无明显差异;而相对于健康患儿其急性期补体C3水平降低,恢复期相对回升,而血清补体C4水平无变化.

  18. Reference Intervals of Serum Complement C3and C4 for 1 to 8 Years Old Healthy Children in Panzhihua Area%攀枝花地区1~8岁健康儿童补体C3、C4含量检测及其参考范围的建立

    Institute of Scientific and Technical Information of China (English)

    严达刚; 段春燕; 董玉红; 杨宝国


    Objective To establish the reference intervals of serum complement C3 ad C4 for 1 to 8 years old healthy children in Pan-zhihua area. Methods Immunoturbidimetry was applied in detecting serum complement C3 and C4 of 912 children of 1 to 8 years old with IMMAGE 800(Beckman-coulter company, USA)special protein analyzer;the results of 7 age stages were statistical analyzed. Re-sults The difference of sex and age in serum complement C3 and C4 among 1 to 8 years old healthy children in Panzhihua area was of no statistical significance(P>0.05), the reference intervals were as follows:C3:0.60~1.82g/L, C4:0.18~0.22g/L, which was of statisti-cal difference from those in“The National Clinical Test Regualation of Operation”and the reference intervals provided by the manufac-turers(P0.05),总的参考范围为C3:0.60~1.82g/L,C4:0.18~0.22g/L。同时与全国临床检验操作规程及厂家提供的参考范围相比较,差异均有统计学意义(P<0.05)。结论全国临床检验操作规程及厂家提供的参考范围已不适应本地区1~8岁儿童血清补体C3、C4的参考范围。各地各实验室应建立各自实验室的参考范围;若存在性别、年龄组间差异,应分别制定性别、年龄各段各自的参考范围。

  19. False elevation of cardiac markers: importance of recognition

    Directory of Open Access Journals (Sweden)

    Yaser Elnahar


    Full Text Available Yaser Elnahar, Joseph Daoko, Karim El Kersh, Jennifer C Kam, Chady Sarraf, Fayez ShamoonSt. Michael’s Medical Center, Newark, NJ, USAAbstract: The availability of troponins as cardiac markers in the diagnosis of acute coronary syndrome is invaluable. However, their elevation can sometimes lead the physician astray. We report a rare case of an 86-year-old Hispanic female with a past medical history significant for asthma, hypertension, atrial fibrillation, and dyslipidemia, who presented to the emergency room complaining of a two-day history of shortness of breath associated with wheezing. She denied any chest pain. The patient’s wheezing ameliorated with bronchodilator treatment. However, her admission laboratory investigations were positive for elevated troponin I, with normal creatine kinase (CK and CK-myoglobin (MB. The first set of cardiac enzymes revealed a troponin I of 29.16 ng/mL (normal < 0.05 ng/mL, CK 234 IU/L, and CK-MB 3.9 IU/L. The electrocardiogram showed rate-controlled atrial fibrillation with nonspecific ST changes. Subsequent cardiac enzymes failed to show any increase in CK or CK-MB. However, the troponin I was, as on admission, persistently elevated at 20.87–29.16 ng/mL. Subsequent cardiac catheterization revealed mild nonobstructive coronary artery disease. Other laboratory tests showed normal creatinine, alkaline phosphatase, and bilirubin, and a negative rheumatoid factor, with absence of hemolysis. A blood sample was subsequently drawn and sent to Beckman Coulter laboratories for heterophile antibody testing. The results confirmed our suspicion of a falsely elevated troponin I caused by the presence of a heterophile antibody. The addition of blocking agents yielded troponin I levels in the normal range. Consistent with current guidelines, we conclude that cardiac markers should be used in conjunction with the clinical picture and the electrocardiogram. This case is unique in that the troponin elevation was

  20. Severe malnutrition with and without HIV-1 infection in hospitalised children in Kampala, Uganda: differences in clinical features, haematological findings and CD4+ cell counts

    Directory of Open Access Journals (Sweden)

    Downing Robert


    Full Text Available Abstract Background The aim of this study was to describe the clinical features, haematological findings and CD4+ and CD8+ cell counts of severely malnourished children in relation to human immunodeficiency virus (HIV infection. Methods The study was conducted in the paediatric wards of Mulago hospital, which is Uganda's national referral and teaching hospital. We studied 315 severely malnourished children (presence of oedema and/or weight-for-height: z-score + and CD8+ cells were measured by the flow cytometry and HIV serology was confirmed by Enzyme linked Immunoassay for children >18 months of age, and RNA PCR was performed for those ≤18 months. Complete blood count, including differential counts, was determined using a Beckman Coulter counter. Results Among the 315 children, 119 (38% were female; the median age of these children was 17 months (Interquartile range 12–24 months, and no difference was observed in the HIV status with regard to gender or age. The children showed a high prevalence of infections: pneumonia (68%, diarrhoea (38%, urinary tract infection (26% and bacteraemia (18%, with no significant difference with regard to the HIV status (HIV-positive versus HIV-negative children. However, the HIV-positive children were more likely to have persistent diarrhoea than the HIV-uninfected severely malnourished children (odds ratio (OR 2.0, 95% confidence interval (CI 1.2–3.6. When compared with the HIV-negative children, the HIV-positive children showed a significantly lower median white blood cell count (10700 versus 8700 and lymphocyte count (4033 versus 2687. The CD4+ cell percentages were more likely to be lower in children with non-oedematous malnutrition than in those with oedematous malnutrition even after controlling for the HIV infection. The novel observation of this study is that the CD4+ percentages in both HIV-positive and HIV-negative children without oedema were lower that those in children with oedema. These

  1. [The influence of dietary fibers on cell immunity under the adequate nutrition and in the presence of alimentary polyhypovitaminosis in rats]. (United States)

    Trushina, É N; Mustafina, O K; Vrzhesinskaia, O A


    The effect of wheat bran on cell immunity in rats adequately provided with vitamins or insufficiently supplied with vitamins has been investigated. 48 male Wistar rats (58.1 +/- 0.5 g) were divided into 6 group and fed with complete semi-synthetic diet, containing 100% or 20% of vitamin mixture (Vit) with or without supplement of insoluble dietary fiber (DF) in the dose corresponding to the upper allowable level of its consumption (5% wheat bran of diet mass) for 4 weeks. The animals of the 1 group received 100% of vitamin mixture (100% Vit); 2 group--100% Vit+DF; 3 group--20% of vitamin mixture (20% Vit); 4 group--20% of vitamin mixture and DF (20% Vit+DF). The next 5 days rats from vitamin-deficient groups were fed with diets supplemented with 80% of Vit: (5 group--20% Vit+80% Vit; 6 group--20% Vit+DF+80% Vit). The contents of lymphocytes, relative quantity of B-(CD45RA+) and T-lymphocytes (CD3+), subpopulations of T-lymphocytes: T-helper (CD3+CD4+) and cytotoxic T-lymphocytes (CD3+CD8+), NK-cells (CD161a+) in the peripheral blood of rats were determined by the method of flow cytometry using Beckman Coulter FC 500 (USA) cytometer. In rats fed complete semi-synthetic diet supplemented with DF (100% Vit+DF) the reduction of relative contents of T-lymphocytes and the increase of the fraction of cytotoxic T-lymphocytes in peripheral blood has been found. The analogous changes and more pronounced degree of immunosupression, that appeared in a lymphocytopenia, much smaller level of T-lymphocytes, T-helper and increase of cytotoxic T-lymphocytes content in rats fed a low vitamins diet (20% Vit) in comparison with these parameters of control group, have been detected. In rats received 20% Vit+DF the suppressed cell immunity was accompanied with decreased level of NK-cells. Normalization of vitamins content in the diets of rat deficient groups led to an almost complete recovery of cell immunity indicators to the level of the animals from the corresponding control groups

  2. 血清补体C3和C4在预测慢性乙型肝炎肝纤维化程度中的价值%Serum complement C3 and C4 levels for predicting severity of hepatic fibrosis in patients with chronic hepatitis B

    Institute of Scientific and Technical Information of China (English)

    张占卿; 陆伟; 王雁冰; 饶敏; 冯洁; 冯艳玲; 沈芳


    目的 探讨血清补体C3和C4预测慢性乙型肝炎肝纤维化程度的价值.方法 选择442例经肝组织病理学检查的慢性乙型肝炎患者.血清补体C3和C4采用Beckman-Coulter Immage 800免疫化学系统及其配套试剂测定.血清补体C3和C4预测肝纤维化程度的评价采用ROC曲线法.结果 血清补体C3和C4预测显著肝纤维化(≥S2)、严重肝纤维化(≥S3)、肝硬化(S4)的ROC 曲线下面积均显著大于标准参考线下面积(P =0.009、0.000、0.000和P=0.005、0.000、0.000).根据ROC曲线,血清补体C3预测严重肝纤维化和肝硬化的最佳截断值分别为≤0.74 g/L和≤0.64 g/L,其对应的灵敏度、特异度、阳性预测值、阴性预测值、准确度分别为0.585、0.681、0.617、0.650、0.636和0.509、0.775、0.423、0.830、0.710;血清补体C4预测严重肝纤维化和肝硬化的最佳截断值分别为≤0.14 g/L和≤0.12 g/L,其对应的灵敏度、特异度、阳性预测值、阴性预测值、准确度分别为0.565、0.634、0.576、0.623、0.602和0.463、0.781、0.407、0.818、0.704.结论 血清补体C3和C4对慢性乙型肝炎严重肝纤维化和肝硬化有一定预测价值,但效能不够稳定和可靠.%Objective To investigate the clinical value of serum complement C3 and C4 levels for predicting the severity of hepatic fibrosis in patients with chronic hepatitis B.Methods Histopathological diagnosis was confirmed in 442 patients with chronic hepatitis B.Serum complement C3 and C4 levels were determined by Beckman-Coulter Immage 800 immunochemistry system.ROC curve was used to analyze the value of serum complement C3 and C4 levels in predicting the severity of hepatic fibrosis.Results The areas under ROC curve of complement C3 and C4 for predicting significant fibrosis ( ≥ S2),severe fibrosis ( ≥ S3) and cirrhosis (S4) were all significantly larger than the area under diagonal reference line ( P =0.009,0.000,0.000 and P =0.005,0.000,0.000,respectively

  3. Clinical evaluation of leukocyte differential count in peripheral blood by five-color flow cytometry%五色流式细胞术分类计数外周血白细胞的临床评价

    Institute of Scientific and Technical Information of China (English)

    邢莹; 王建中; 普程伟; 尚柯


    Peking University First Hospital from September 2010 to December 2010 was detected by standard microscopic cytology as a reference method.Meanwhile,Beckman-Coulter LH750 hematology analyzer and FC500 flow cytometer were performed.Then the correlations were analyzed between microscopic cytology,hematology analyzer and flow cytometry.Forty blood samples collected at Peking University First Hospital,Beijing Daopei Hospital and General Hospital of Beijing Military Command from August 2010 to November 2010 were analyzed by standard microscopic cytology,Beckman-Coulter LH750 hematology analyzer and NAVIOS flow cytometer.Then the correlations between microscopy,hematology analyzer and flow cytometry were explored to analyze the clinical diagnostic efficiency of flow cytometry.Results Correlation of leukocyte differential count between FC500 flow cytometer and standard microscopic cytology was significant(all P < 0.01).And it was superior in the detection of lymphocytes,neutrophils and eosinaphils (r =0.955,0.928,0.876).Moreover,the correlation of leukocyte differential count between NAVIOS flow cytometer via manual gate and standard microscopic cytology was significant (r > 0.700,all P < 0.01) except for basophils.And it was superior in the detection of neutrophils,lymphocytes and blasts (r =0.950,0.915,0.852).When 1% was set as the cut-off value of immature granulocytes on standard microscopic cytology,the sensitivity and specificity of flow cytometry was 87% and 76% respectively.When 0.5% was set as the cut-off value of blasts on standard microscopic cytology,the sensitivity and specificity of flow cytometry stood at 100% and 92% respectively.Conclusion Five-color flow cytometry is wellcorrelated with standard microscopic cytology for leukocyte differential count in peripheral blood with different flow cytometers,and the sensitivity of detecting blasts and immature granulocytes is very excellent.

  4. Determining the Dimension of Knowledge Management Implementation by Utilizing a Fuzzy TOPSIS Method


    Mona Ahani; Hamid Reza Bahrami; Behrooz Shariflu


    The purpose of this research is to identify and rank different aspects of knowledge management based on the Beckman model using the Fuzzy TOPSIS technique. The Beckman model, in this research, has 8 main dimensions including: knowledge identification, knowledge acquisition, knowledge selection, knowledge storage, knowledge distribution, knowledge creation, knowledge application, knowledge business, and 33 sub-criteria or alternatives. The population of the present study includes 197 members o...

  5. Comparison of a new rapid plasma reagin card test with the standard rapid plasma reagin 18-mm circle card test and the venereal disease research laboratory slide test for serodiagnosis of syphilis.


    Hambie, E A; Larsen, S A; Perryman, M W; Pettit, D E; Mullally, R L; Whittington, W


    The rapid plasma reagin (RPR) card test manufactured by Beckman Instruments, Inc., was compared, qualitatively and quantitatively, with the Venereal Disease Research Laboratory (VDRL) slide test and the standard RPR 18-mm circle card tests for the serodiagnosis of syphilis. Sera from 638 individuals were used in this study. Two pilot lots and two production lots of antigen were submitted by Beckman Instruments, Inc., for evaluation. Qualitative agreement among the three RPR card tests was 98....

  6. Elevated levels of beta-human chorionic gonadotropin and human placental lactogen between 11-13 week's gestation and subsequent pregnancy complications in Oman i women

    International Nuclear Information System (INIS)

    The association between abnormal levels of maternal serum b-human chorionic gonadotrophin (b-HCG) and human placental lactogen (HPL) measured in early pregnancy and future poor pregnancy outcome is fairly well established. Little is known on how such prognostic information can be used to avoid future complications of pregnancy and improve their outcome. We undertook a prospective study, which was designed to assess the efficiency of maternal serum biochemical markers b-HCG and HPL for the detection of different pregnancy and labor complications. The blood samples were taken from pregnant patients who attended the antenatal clinic at Sultan Qaboos University Hospital in Muscat, Sultanate of Oman, from December 2001 to October 2002. These patients were selected randomly. A total of 200 Omani women, none diabetic, with singleton pregnancies between 11 and 13 weeks gestation were recruited into the study. Gestational age was calculated from the first day of the last menstrual period, unless ultrasonography showed a discrepancy of more than 14 days. Excluded from the study were pregnancies with fetal anomalies, multiple gestations and insulin dependent maternal diabetes. Biochemical analysis of both maternal serum markers was performed in the clinical biochemistry laboratory using an automated immunometric technique supplied by Beckman Coulter for b-HCG and manual radioimmunoassay utilizing a gamma radiation scintillation counter for HPL. Both assays underwent internal, trilevel quality controls. The selected patients were monitored for the following complications: pregnancy induced hypertension (PIH), gestational diabetes, polyhydramnios, antepartum hemorrhage, intrauterine growth retardation (IUGR) (birth weight <10th per centile for gestation), low Apgar score (7 or less) and emergency cesarean section. 75 patients did not develop any pregnancy complications and delivered vaginally. This was our control group. 85 patients developed one of the above mentioned problems

  7. 碱性磷酸酶对肌钙蛋白Ⅰ测定干扰的评价%Interference of alkaline phosphatase on clinical cardiac troponin Ⅰ assay

    Institute of Scientific and Technical Information of China (English)

    刘泽金; 郑芳; 张真路; 范庆坤; 王纯


    目的 评价ALP对cTnI测定的干扰.方法 制备1份正常混合血浆和2份不同浓度cTnI的异常混合血浆,各分成8组,第1组为对照组,另7组加入不同浓度ALP,分别在ACCESS2 (Beckman Coulter公司)及AXSYM(美国Abbott公司)上测定每组标本的cTnI和ALP,并与对照组cTnI比较,评价ALP是否对cTnI测定产生干扰.结果 ACCESS cTnI测定正常血浆时,ALP浓度高达3 716 U/L时测定结果为(0.04±0.01)μgL,与正常血浆组的(0.04±0.01)μg/L比较差异无统计学意义(t=0.40,P>0.05);ALP高于917 U/L时,AXSYM cTnI测定结果为(0.08±0.01)μg/L,高于正常血浆组的(0.04±0.01)μg/L,差异有统计学意义(t=-4.89,P<0.01);ACCESS cTnI测定异常血浆时,ALP浓度高达3 534 U/L时,cTnI测定结果为(13.41±0.17)μg/L,与正常血浆组的(13.48±0.16)μg/L比较,差异无统计学意义(t=0.52,P>0.05).结论 高浓度ALP对ACCESS cTnI测定无干扰,不会导致假阳性结果,但高浓度ALP(917 U/L以上)会造成AXSYM cTnI检测干扰导致假阳性结果.%Objective To evaluate the interference of ALP on cTnI assays. Methods One normal mixed plasma sample and 2 abnormal mixed plasma samples with different cTnI levels were prepared, and then divided them into 8 groups respectively. One group was randomly chosen as control while different amounts of ALP were added into the other seven groups. The concentrations of cTnI and ALP in each plasma portion were detected by ACCESS2 (Beckman-Coulter, Inc ) and AXSYM (Abbott Laboratories )separately. The results of the seven tested groups were then compared with those of the control, so as to evaluate whether ALP could interfere with the cTnI assay. Results When the chemiluminescent Access cTnI assay was carried out for detection of normal plasma, the concentration of ALP was up to 3 716 U/L and did not interfere with the test results of cTnI [(0. 04 ±0.01) μg/L] compared with those of the control portion [(0. 04 ± 0. 01 ) μg/L] (t = 0. 40, P > 0. 05 ). Once

  8. Rome

    Directory of Open Access Journals (Sweden)

    Jan Nelis


    Full Text Available Apted, Michael; Farino, Julia; Coulter, Allen et al. (dirs.; HBO/BBC (prod.; Heller, Bruno; Milius, John; Frankel, David; Macdonald, William J.; Cunningham, Alexandra and Hodges, Adrian (script. United Kingdom, 2005: son., col.

  9. Karussellile väljavalitud / Aita Kivi

    Index Scriptorium Estoniae

    Kivi, Aita, 1954-


    Sisu : Eeva Park. Pääse karussellile; Waris Dirie, Cathleen Miller. Kõrbelill; Colette Davenat. Väljavalitu; Arthur Hailey. Kõrged ametikohad; Mari Saat. Sinikõrguste tuultes; Kogumik "Inimese vägi"; Catherine Coulter. Elu ja surma piiril

  10. ECOHAB: Kamykowski_D- Florida Process Cruise 11/16-19/1998- Karenia brevis counts, biochemistry and behavior (NODC Accession 0000533) (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Abstract: Water bottle samples were collected from selected depths (variable with station) at 44 stations in the study area. Coulter counts in the 14 to 28 um size...

  11. ECOHAB: Kamykowski_D- Florida Process Cruise 11/16-19/1998- Karenia brevis counts, biochemistry and behavior (NODC Accession 0000534) (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Abstract: Water bottle samples were collected from selected depths (variable with station) at 14 stations in the study area. Coulter counts in the 14-28 um size...

  12. Força de tração e volume de solo mobilizado por haste sulcadora em semeadura direta sobre campo nativo, em função do teor de água no solo, profundidade e velocidade de operação Draft and soil loosening by knife type coulter related to soil moisture and planter's working speed and depth


    Carla T. C. Cepik; Carlos R. Trein; Renato Levien


    A semeadura direta é um sistema de manejo eficiente em termos de conservação do solo e da água, economia de energia e capacidade operacional dos conjuntos mecanizados. No entanto, em áreas onde existem pecuária e produção de grãos, ou onde o solo é trafegado e trabalhado em condições inadequadas, pode ocorrer compactação do solo nas camadas superficiais. Mesmo compactadas, essas camadas não necessitariam ser descompactadas ou revolvidas, se boa germinação e desenvolvimento da cultura fossem a...

  13. Setí obilovin a faktory ovlivňující přesnost uložení semen do půdy


    Jungwirthová, Zuzana


    The aim of my diploma thesis was to ascertain the applicability of the test drill coulter for precision sowing of cereals and other crop plants. The thesis summarizes the requirements on the drill coulter which could be used for precision sowing of cereals in the future. A part of the study defines the terms associated with the individual sowing characteristics, including a historical overview of the methods of sowing cereals and other crop plants. The study further describes the factors b...

  14. Ekoodlaren


    Tobiasson, Mats


    The Ekoodlaren (Eco cropper) is a new implement constructed to prepare the seedbed, to seed, to fertilize and to row weed in single or combined operations. The main purposes for the construction is to save time and money, and to reduce excessive packing of the soil. Its drilling and row hoeing qualities were examined at the Department of Soil Sciences in three series of field trials during 1991. Two of the series, 'Seed coulters: Different tillage methods' (Rl-5015) and 'Seed coulters: Minimi...

  15. Structure and tectonics of the southwestern continental margin of India

    Digital Repository Service at National Institute of Oceanography (India)

    Subrahmanyam, V.; Rao, D.G.; Ramana, M.V.; Krishna, K.S.; Murty, G.P.S.; Rao, M.G.

    with ages (Courtillot et al., 1986; Beckman et al., 1988). Dots with numbers indicate ODP sites. CLR = Chagos-Laccadive Ridge; NR = Ninety-east Ridge; LR = Laxmi Ridge; BH = basement high; PR = Prathap Ridge; MBR = mid-shelf basement ridge. (b) Hotspot..., 1982). The Mascarene-Chagos-Laccadive volcanic lin- eament is a major aseismic ridge system that con- nects the young volcanic activity of the Reunion hotspot (Beckman et al., 1988). This lineament paral- lels the remarkable Ninety-east Ridge which...

  16. Evaluation of official instrumental methods for the determination of particulate matter contamination in large volume parenteral solutions. (United States)

    Montanari, L; Pavanetto, F; Conti, B; Ponci, R; Grassi, M


    The distribution pattern of particle contamination in nine different types of LV parenteral solutions and the possibility of correlating the counts made with two official instruments (Coulter Counter and HIAC) were studied. Two hundred containers of LV parenteral solutions (corresponding to 40 batches) produced in Italy, were sampled. Each bottle was submitted to HIAC and Coulter Counter countings, for particle sizes ranging between 2 and 25 micron. For about 50% of the products, the two straight lines that represent the distribution of particle contamination obtained with the two methods did not cross-over within the studied size range, the Coulter Counter counts always proving higher than the HIAC ones. In the other cases, the cross-over point of the two lines occurred at varying size levels. Statistical analysis of the results pointed to a relationship between the contamination values obtained with the two counting methods for sizes ranging between 2 and 5 micron, but there was no correlation for sizes equal to, or higher than, 10 micron. From the maximum contamination levels established by the BP and the FU IX for the HIAC method, the corresponding values were calculated for the Coulter Counter method. Similarly the values were calculated the HIAC method based on the maximum values set for the Coulter Counter. PMID:2879006

  17. Differential microfluidic sensor on printed circuit board for biological cells analysis. (United States)

    Shi, Dongyuan; Guo, Jinhong; Chen, Liang; Xia, Chuncheng; Yu, Zhefeng; Ai, Ye; Li, Chang Ming; Kang, Yuejun; Wang, Zhiming


    Coulter principal based resistive pulse sensor has been demonstrated as an important platform in biological cell detection and enumeration since several decades ago. Recently, the miniaturized micro-Coulter counter has attracted much attention due to its advantages in point of care diagnostics for on chip detection and enumeration of rare cells, such as circulating tumor cells. In this paper, we present a microfluidic cytometer with differential amplifier based on Coulter principle on a SU-8 coated printed circuit board substrate. The electrical current changes induced by the blockage of the microparticles in the sensing aperture are calibrated by polystyrene particles of standard size. Finally, HeLa cells are used to evaluate the performance of the proposed device for enumeration of biological samples. The proposed cytometer is built upon the cheap and widely available printed circuit board substrate and shows its great potential as personalized healthcare monitor. PMID:25735615

  18. Course Management Systems and Blended Learning: An Innovative Learning Approach (United States)

    Chou, Amy Y.; Chou, David C.


    This article utilizes Rogers' innovation-decision process model (2003) and Beckman and Berry's innovation process model (2007) to create an innovative learning map that illustrates three learning methods (i.e., face-to-face learning, online learning, and blended learning) in two types of innovation (i.e., incremental innovation and radical…

  19. Organizational Perceptions of Telecommuting in the Private Sector (United States)

    Galusha, Repps J.


    The Internet has provided more organizations the opportunity to adopt telecommuting as a means to recruit and retain employees, boost productivity, and trim facility costs. This study expands on the work of a previous study by Hoang, Nickerson, Beckman, and Eng, in 2008 which found that private organizations, due to perceptions of organizational…

  20. Determination of spectrophotometric absorptivity by analytical ultracentrifugation

    Directory of Open Access Journals (Sweden)

    M Senthilraja


    Full Text Available Rapid determination of the absorptivity for a recombinant IgG monoclonal antibody using the Beckman equipped with both Raleigh interference and UV absorbance optical systems. The analytical ultracentrifuge data for determining spectrophotometric absorptivities is compared to experimental data from quantitative amino acid analysis and an enzymatic digestion method.

  1. Particles in small volume injections. (United States)

    Taylor, S A; Spence, J


    The level of particulate contamination in small volume injections has been examined using the light blockage (HIAC) and electrical sensing zone (Coulter counter) techniques, the HIAC system being found to be the more suitable. Particle counts on the same batch of injection showed a large and variable difference between the HIAC and the Coulter counter results, especially below 5 micron. None of the injections examined complied with the British Pharmacopoeia limits for particulates in large volume parenterals, suggesting the unsuitability of the limits for small volume parenterals. PMID:6141237

  2. 不同地区招飞体检学生静脉血细胞参数对比分析%Comparison on the venous blood cells parameters of the candidates in physical examination for pilot recruitment in different areas

    Institute of Scientific and Technical Information of China (English)

    厉淑红; 郑军; 周金立; 马新伟


    Objective To analyze the difference and variation rule of the parameters of venous blood cells in the pilot candidates from different areas,and provide a reference for the revision of physical examination standard and physical evaluation.Methods The candidates were divided into seven groups:East China,South China,Central China,North China,Northwest China,Southwest China,and Northeast China,according to the areas they came from,and 224 candidates were randomly selected from each group.2 ml morning fasting venous blood was collected into a dipotassium ethylene tetraacetate anticoagulation tube.As the sample was mixed homogenously,parameters of blood cells were determined by Beckman Coulter system 2 Automated Hematology Analyzer and the results were shown in the form of mean ± standard deviation (x±s).The statistical analysis was performed by using one-way analysis of variance (ANOVA),and it took P<0.05 as the statistical significance of difference.Results Comparing the blood cell parameters of the candidates from different areas,WBC,neutrophilic granulocyte,lymphocyte,and monocyte showed no statistical significance (P > 0.05);eosinophilic granulocyte and basophilic granulocyte showed statistical significance (F=7.906,2.524,P<0.01);all parameters of red blood cell and platelet showed statistical significance (F=4.435-14.864,F=4.847-20.657,P<0.01).The percentage of abnormality was respectively 18,11% in RBC,43.56% in Hb,14.16% in hemoglobin,17.54% in lymphocyte,9.06% in neutrophilic granulocyte,and 1.40% in platelet.Conclusions There are regional differences in venous blood cell parameters among the pilot candidates from different areas.It is necessary to improve the items of blood cell test and the evaluation standard considering the area difference in order to obtain more accurate physical evaluation and the references for the tracking survey of the blood cell parameters in pilots.%目的 分析我国不同地区招飞体检学生静脉血细

  3. Proxies and measurement techinques for mineral dust in antarctic ice cores

    DEFF Research Database (Denmark)

    Ruth..[], Urs; Bigler, Matthias


    To improve quantitative interpretation of ice core aeolian dust records, a systematic methodological comparison was made. This involved methods for water-insoluble particle counting (Coulter counter and laser-sensing particle detector), soluble ion analysis (ion chromatography and continuous flow...

  4. Optimization, Application, and Interpretation of Lactate Dehydrogenase Measurements in Microwell Determination of Cell Number and Toxicity

    NARCIS (Netherlands)

    Wolterbeek, H.T.; Van der Meer, A.J.G.M.


    The lactate dehydrogenase (LDH) assay was addressed for its sensitivity, disturbances by foaming, and cell number and size. Cells were from a U-251 MG grade IV human glioblastoma brain tumor cell line used in 100-µl well volumes. Cells were counted by microscopy and Coulter counting; assays were LDH

  5. 2x Ben Affleck / Laurence Lumiere

    Index Scriptorium Estoniae

    Lumiere, Laurence


    Põnevusfilm "Hollywoodland" : režissöör Allen Coulter : Ameerika Ühendriigid 2006 ja põnevuskomöödia "Äss mättasse" ("Smokin' Aces") : režissöör Joe Carnahan : Suurbritannia - Prantsusmaa - Ameerika Ühendriigid 2007. Mõlemas mängib Ben Affleck

  6. Pikantsed pajatused / Aita Kivi

    Index Scriptorium Estoniae

    Kivi, Aita, 1954-


    Sisu : Louis Paul Boon. Menuett; Herbjırg Wassmo. Õnne poeg; Jean Stone. Sünnipäevatüdrukud; Jo︠l Schmidt. Constantinus Suure mälestused; Mari Vallisoo. Ainsuse olevik; Catherine Coulter. Märklaud; Ross Macdonald. Mees mulla all

  7. Preadipocyte proliferation is repressed by miR-15a targeting “the preadipocyte factor” DLK1

    DEFF Research Database (Denmark)

    Andersen, Ditte Caroline

    , and is generally referred to as “the” preadipocyte marker. The DLK1 gene itself is situated in one of the largest known miRNA clusters, yet, no reports have been made on miRNAs targeting Dlk1. By examinating preadipocytes in vitro using flowcytometry, ELISA, coulter cell counting, immunocytochemistry...

  8. Off-equatorial circular orbits in magnetic fields of compact objects

    Czech Academy of Sciences Publication Activity Database

    Stuchlík, Z.; Kovář, J.; Karas, Vladimír

    Cambridge : Cambridge University Press, 2009 - (Strassmeier, K.; Kosovichev, A.; Beckman, J.), s. 125-126 ISBN 978 0521-88990-2. ISSN 1743-9213. - (IAU Symposium Proceeding Series. 259). [Symposium of the International Astronomical Union /259./. Puerto Santiago, Tenerife (ES), 03.11.2008-07.11.2008] Institutional research plan: CEZ:AV0Z10030501 Keywords : black hole physics * magnetic fields * neutron stars Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics

  9. Simplified and approximated correlations of heat transfer effectiveness for steam condenser


    Rafal Marcin Laskowski; Janusz Lewandowski


    This paper presents two simplified and approximated relations of heat transfer effectivneness for steam condenser. The proposed relations of heat transfer effectivneness are a function of the measured and reference parameters. Correctness of relations were verified on the basis of data from the characteristics and measured data for the 200 MW steam condenser. A comparison of the outlet cooling water temperature calculated from the proposed relations and Beckman formula were performed. Formula...

  10. Evaluation of four gentamicin and tobramycin assay procedures for clinical laboratories.


    Witebsky, F G; Sliva, C A; Selepak, S T; Ruddel, M E; MacLowry, J D; Johnson, E E; Elin, R J


    Accuracy, precision, and clinical laboratory utility of the TDX (Abbott Laboratories), Auto-ICS (Beckman Instruments, Inc.), COBAS-Bio (Roche Analytical Instruments, Inc.) with reagent kits (Syva), and EMIT (Syva) for gentamicin and tobramycin serum assay were assessed. TDX, COBAS-Bio, and EMIT analytical systems showed a proportional bias of less than 10% for recovery studies and a coefficient of variation less than 5% for within-run precision. The results of the recovery studies with the Au...

  11. Emerging role of brain metastases in the prognosis of breast cancer patients


    Hambrecht A; Jandial R; Neman J


    Amanda Hambrecht1,2, Rahul Jandial2, Josh Neman21Department of Biology, University of Southern California; 2Department of Neurosurgery, Beckman Research Institute, City of Hope National Cancer Center, CA, USAAbstract: Cancer starts with one rogue cell. Through mutations and genomic alterations, the cell acquires specific and stem cell-like characteristics necessary for invasion of a distant organ and ultimately metastasis. Metastatic brain cancer is a particularly formidable disease because o...

  12. The Protein and Nucleic Acid (PAN) Facility at Stanford University


    Eckart, M.; Kosovilka, N.; Sanchez, A; Tran, Y; Walker, P; Winant, R.; Zuo, E.; Patel, S.


    The Protein and Nucleic Acid (PAN) Facility ( at Stanford University's Beckman Center is a multifaceted biotechnology fee-for-service laboratory providing services to the Stanford scientific community, other non-profit and biopharmaceutical organizations. The Facility's mission is to be adaptable and responsive to the changing needs of biomedical research by providing basic science investigators continued access to key tools and applications in an efficient and cost ef...

  13. Electro-magnetic fields around a drifting Kerr black hole

    Czech Academy of Sciences Publication Activity Database

    Kopáček, Ondřej; Karas, Vladimír

    Cambridge: Cambridge University Press, 2009 - (Strassmeier, K.; Kosovichev, A.; Beckman, J.), s. 127-128. (IAU Symposium Proceeding Series. 259). ISBN 978 0521-88990-2. ISSN 1743-9213. [Symposium of the International Astronomical Union /259./. Puerto Santiago, Tenerife (ES), 03.11.2008-07.11.2008] Institutional research plan: CEZ:AV0Z10030501 Keywords : black hole physics * magnetic fields * relativity Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics

  14. Two-stage cell shrinkage and the OER for radiation-induced apoptosis of rat thymocytes

    International Nuclear Information System (INIS)

    Suspensions of rat thymocytes were given 0.09-100 Gy using 60Co γ-rays. The radiation-induced changes in the thermocytes were examined from minutes to hours post-irradiation using electron microscopy, agarose gel electrophoresis, staining and Coulter Counter sizing. Sizing by Coulter Counter showed, for the first time, that thermocytes which undergo apoptosis shrink in two distinct stages, first by a sudden decrease from an original volume of 99 μm3 to a volume of 76 μm3, followed by a gradual decrease to 57 μm3 over the space of a few hours. The oxygen enhancement ratio for apoptosis was measured to be about 3.5, similar to the value for reproductive death for many mammalian cells. (author)

  15. Blood haemoglobin concentrations are higher in smokers and heavy alcohol consumers than in non-smokers and abstainers-should we adjust the reference range?

    DEFF Research Database (Denmark)

    Milman, N.; Pedersen, Agnes N.


    The blood haemoglobin concentration is one of the most frequently used laboratory parameters in clinical practice. There is evidence that haemoglobin levels are influenced by tobacco smoking. The objective of this study was to evaluate the impact of smoking and alcohol consumption on haemoglobin...... concentrations in apparently healthy subjects living at sea level. A retrospective, epidemiological cohort study was performed in 1984. Participants were 1,503 men and 1,437 non-pregnant women evenly distributed in age cohorts of 30, 40, 50, and 60 years. Information of smoking habits and alcohol consumption...... were obtained by a questionnaire. Haemoglobin was measured in the fasting state on Coulter-S. Men displayed no difference in mean haemoglobin levels in the four age groups. In women, mean haemoglobin increased gradually with age (p = 0.001). Fifty-nine percent of men and 50% of women were daily smokers...

  16. Lower Total Adipocyte Number but No Evidence for Small Adipocyte Depletion in Patients With Type 2 Diabetes


    Pasarica, Magdalena; Xie, Hui; Hymel, David; Bray, George; Greenway, Frank; Ravussin, Eric; Smith, Steven R.


    OBJECTIVE We hypothesized that, compared with obese subjects, patients with type 2 diabetes have a lower total adipocyte number with fewer small adipocytes. RESEARCH DESIGN AND METHODS Abdominal subcutaneous adipose tissue was obtained from lean and obese subjects with or without type 2 diabetes matched for BMI. Adipocyte size was measured by osmium fixation and sizing/counting in a Coulter counter. Adipocyte size and number subdistributions (small, medium, large, and very large) were determi...

  17. Biosensor Scheme for the Determination of Intracellular Pressure of Erythrocyte

    Directory of Open Access Journals (Sweden)

    Yu.S. Nagornov


    Full Text Available The paper presents a scheme of the biosensor for determining the intracellular pressure of erythrocytes. The possibility of measuring of the volume and area of the erythrocyte is provided in a biosensor to determine the value intracellular pressure. In MEMS this creates flow that enters into Coulter capacitive sensor through the rate control system and then in the system of signal transmitting. The definition of erythrocyte volume and calculation of intracellular pressure occur in the computer system.

  18. Short term variations in feeding and metabolism of Acartia tonsa (pelagic copepod) in the Berre lagoon (France)


    Gaudy, R.; Pagano, M; Cervetto, G; Saintjean, L; Verriopoulos, G; Beker, B.


    Feeding, respiration, and ammonia excretion of Acartia tonsa were studied in a brackish lagoon, near Marseilles (Southern France). The experiments were performed during the same season in two successive years, using naturally occurring particles as food. Strong variations in both quantity and quality of food particles were recorded between the two series of experiments. The ingestion of particles according to their size was studied by means of the Coulter Counter technique. Females ingested m...

  19. A manual bead assay for the determination of absolute CD4+ and CD8+ lymphocyte counts in human immunodeficiency virus-infected individuals.


    Carella, A V; Moss, M.W.; Provost, V; Quinn, T C


    CD4+ T lymphocytes are currently the most common surrogate marker indicating disease progression in individuals infected with human immunodeficiency virus (HIV). Since the cost of enumerating lymphocyte phenotypes is quite high, an inexpensive bead assay analyzed by light microscopy (cytosphere assay; Coulter Corporation, Hialeah, Fla.) was developed as an alternative method for counting CD4+ and CD8+ T lymphocytes. To evaluate the reliability of the cytosphere assay, heparinized blood was co...

  20. Miniaturization of powder dissolution measurement and estimation of particle size. (United States)

    Avdeef, Alex; Tsinman, Konstantin; Tsinman, Oksana; Sun, Na; Voloboy, Dmytro


    The objective was to investigate the applicability and limitations of an approach for estimating particle size from powder dissolution measurement using as little as 50 microg of sample in 1 ml of buffer solutions. The powder dissolution profiles of five sparingly-soluble drugs (hydrochlorothiazide, phenazopyridine hydrochloride, 2-naphthoic acid, indomethacin, and dipyridamole) were evaluated with a novel biexponential spherical particle equation and also the Wang-Flanagan spherical particle non-sink equation. The results were compared to particle sizing based on measured specific surface area by the Brunauer-Emmett-Teller (BET) method, and also based on Coulter counting. With the exception of hydrochlorothiazide, the model compounds indicated some agglomeration in the dissolution media. The dry-state specific surface area was larger than expected from either the Coulter method or the powder-dissolution data, especially for phenazopyridine hydrochloride. The particle radii estimated by the powder dissolution method ranged from 10 to 68 microm, with equilibrium solubilities spanning from 5 microg/ml (dipyridamole) to 911 microg/ml (hydrochlorothiazide). Powder dissolution data collected with the miniaturized apparatus can be used to determine particle size, with estimated values agreeing reasonably with those measured by the Coulter counter method. PMID:19937817

  1. Determination of average molecular weights on organic reactor coolants. II.-Freezing point depression method for diphenyl-ether solutions

    International Nuclear Information System (INIS)

    In order to reduce limitations of solubility, the cryoscopic method developed for benzene solutions of polyphenyl mixtures has been extended to diphenyl-ether solutions by introducing some modifications imposed by the physico-chemical properties of this solvent. The Nernsto theory of Beckman's method has been revised, taking into account the heat-transfer characteristics of the system, and the results of that analysis have been used to fix upon the design parameters of a cryoscopic apparatus for measurements on diphenyl-ether solutions. (Author) 9 refs

  2. Solar energy in the United States

    International Nuclear Information System (INIS)

    Written by a group of five French experts who visited several research centres, innovating companies and solar power stations in the United States, this report first proposes an overview of solar energy in the United States, indicating and commenting the respective shares of different renewable energies in the production, focusing on the photovoltaic energy production and its RD sector. The second part presents industrial and research activities in the solar sector, and more specifically photovoltaic technologies (silicon and thin layer technology) and solar concentrators (thermal solar concentrators, photovoltaic concentrators). The last chapter presents the academic research activities in different universities (California Tech Beckman Institute, Stanford, National Renewable Energy Laboratory, Colorado School of Mines)

  3. Observations on the morphology and chemical analysis of medullary granules in chinchilla hair. Research letters

    Energy Technology Data Exchange (ETDEWEB)

    Keogh, H.J. (South African Inst. for Medical Research, Johannesburg); Haylett, T. (Council for Scientific and Industrial Research, Pretoria (South Africa). National Chemical Research Lab.)


    The ultrastructure of the medullary granules of white and grey chinchilla hair was investigated by scanning electron microscopy and chemical analysis in an attempt to clarify their structure and function. Atomic absorption spectroscopy and amino acid analysis showed them to be composed of melanin. The sample preparation for scanning electron microscopy is discussed. The metal content was qualitatively established by X-ray fluorescence spectrometry and quantitatively determined on a Varian Techtron model AAs atomic absorption spectrophotometer. Amino acid analysis of the granule, was carried out on a Beckman 121 amino acid analyser. Information is provided on the amino acid composition of the medullary granules as well as its metal content.

  4. A beam condenser for infrared spectrophotometers. (United States)

    Brandt, R C


    The design and performance of a beam condenser for ir spectrophotometers such as the Beckman model IR-11 is described. The instrument has an image size of 4 mm x 8 mm and permits the use of samples mounted in the tail of a cryostat whose outside dimension is as large as 7 cm square. Applications to negative light flux spectroscopy are described, in particular, the direct measurement of the longitudinal optical frequency of lattice vibration for AgBr and AgCl. PMID:20072220

  5. Determination of average molecular weights on organic reactor coolants. II.-Freezing point depression method for diphenyl-ether solutions; Determinacion de masas moleculares medias en refrigerantes nucleares organicos. II.- Crioscopia de disoluciones en eter difenilico

    Energy Technology Data Exchange (ETDEWEB)

    Carreira, M.


    In order to reduce limitations of solubility, the cryoscopic method developed for benzene solutions of polyphenyl mixtures has been extended to diphenyl-ether solutions by introducing some modifications imposed by the physico-chemical properties of this solvent. The Nernsto theory of Beckman's method has been revised, taking into account the heat-transfer characteristics of the system, and the results of that analysis have been used to fix upon the design parameters of a cryoscopic apparatus for measurements on diphenyl-ether solutions. (Author) 9 refs.

  6. Observations on the morphology and chemical analysis of medullary granules in chinchilla hair

    International Nuclear Information System (INIS)

    The ultrastructure of the medullary granules of white and grey chinchilla hair was investigated by scanning electron microscopy and chemical analysis in an attempt to clarify their structure and function. Atomic absorption spectroscopy and amino acid analysis showed them to be composed of melanin. The sample preparation for scanning electron microscopy is discussed. The metal content was qualitatively established by X-ray fluorescence spectrometry and quantitatively determined on a Varian Techtron model AAs atomic absorption spectrophotometer. Amino acid analysis of the granule, was carried out on a Beckman 121 amino acid analyser. Information is provided on the amino acid composition of the medullary granules as well as its metal content

  7. A comparison of analytical methods for detection of [14C]trichloro acetic acid-derived radioactivity in needles and branches of spruce (Picea sp.)

    International Nuclear Information System (INIS)

    The branches (wood and needles) of spruces of varying age treated with [14C]trichloro acetic acid (3.7 GBq/mmol) were studied, using the following methods: Qualitative: - Conventional macroautoradiography with X-ray film and histological classification. Quantitative: - 14C combustion analysis with the sample oxidizer A 307 (Canberra/Packard) followed by measurement of radioactivity using the LS counter 6000 (Beckman Instrumentts); - digital autoradiography with the Digital Autoradiograph LB 286 (Berthold GmbH); -digital autoradiography with the Bio-imaging Analyzer BAS 2000 (Fuji Film Co.). (orig.)

  8. Contornos de entonación en el discurso de una docente. Su relación con algunos aspectos pragmáticos

    Directory of Open Access Journals (Sweden)

    Guadalupe Álvarez


    Full Text Available En este artículo se describen los contornos de los grupos entonacionales del discurso de una docente con el objeto de determinar las características que adoptan en relación con algunos aspectos pragmáticos relevantes de ese discurso, como partículas de iniciación, continuidad, completud y diferentes actos de habla (aseveraciones, órdenes, pedidos o sugerencias y, sobre todo, distintas clases de preguntas. Para ello, realizo una descripción perceptual y físico-acústica de 172 grupos entonacionales combinando el sistema TOBI de notación (Beckman y Ayers Elam, 1993 con la marcación de valores ERB y la posición silábica (Gurlekian y otros, 1999. (The aim of this article is describe the contours of the intonational groups of a teacher’s discourse in order to determine the characteristics that they adopt in relation to some relevant pragmatic aspects of it, included initial particles, continuity, completion and different speech acts. To achieve this, I describe 172 intonational groups from an auditory and physical-acoustic analyses, combining the TOBI system notation (Beckman y Ayers Elam, 1993 with the ERB-rate scale and the syllabic position (Gurlekian et al, 1999.

  9. Comparison and harmonization assessment of mutual among four different immunoassay systems in CA19-9 test%CA19-9测定在4个免疫检测系统的差异及互认能力的评估

    Institute of Scientific and Technical Information of China (English)

    宋超; 张曙云; 周俊; 单志明; 周永列


    目的 研究4个免疫检测系统对CA19-9检测的差异和一致性,探讨实现CA19-9检测结果互认的基础.方法 检测系统比对研究.汇集整理CA19-9室间质量评价活动批号的数据(卫生部200811 ~ 201215,浙江省080309 ~ 120211);搜集120份不同CA19-9浓度的新鲜血样本;搜集2010年9月至2012年3月不同检测系统非肿瘤体检人群实测CA19-9数据.选择4个稳定的检测系统Abbott Architect i2000、Roche E170、Beckman Dxi800和Siemens CentaurXP作为研究对象,统计分析在卫生部和浙江省室间质评(冻干粉和液体血清)中的组间差异;参照CLSI EP9-A2进行120份新鲜血样本的比对分析;计算各检测系统实测CA19-9的95%置信区间并分析.采用散点图、加权回归方程进行统计比较.结果 室间质评与新鲜血比对出现颠倒的结果.室间质评均表现出检测系统组间较大的偏倚,斜率bw在1.340到4.683之间,相关系数r较好;新鲜血比对中r不理想,但斜率bw接近于1.新鲜血比对中在推荐浓度27 U/ml时,检测系统两两间偏移分别为Abbott-Roche(-6.41%),Beckman-Roche(-5.07%),Siemens-Roche(13.15%),Beckman-Abbott(2.46%),Siemens-Abbott(22.52%),Siemens-Beckman(39.66%).部分检测系统间实测95%置信区间差异有统计学意义.结论 在研究检测系统差异时,在较低浓度CA19-9的结果在4个检测系统间具有互认的基础,但在高浓度时,检测系统间的差异明显增大,风险较高,尚无法实现互认.%Objective To investigate the differences and harmonization of immunoassay systems in detecting CA19-9 and to assess the possibility of mutual recognition in different laboratories.Methods Data were collected and analyzed from External Quality Assessments (EQA) of NCCL(Lots:200811-201215) and ZJCCL(Lots:080309-120211).120 fresh serum with different concentrations of CA19-9 were collected.The CA19-9 results of healthy people were also collected from September 2010 to March

  10. Glaciological and chemical studies on ice cores from Hans Tausen ice cap, Greenland

    DEFF Research Database (Denmark)

    Clausen, H.B.; Stampe, Mia; Hammer, C.U.;


    The paper presents studies of various chemical and isotopical parameters from ice cores drilled in the northernmost located ice cap, Hans Tausen Iskappe, Pearyland, Greenland (HT). The 346 m main core (MC95) was drilled to bedrock in 1995 as well as a 35 m shallow core (SC95). A 60 m shallow core...... selected for an analysis of dust and water soluble chemical components, including F-, CH3SO2-, Cl-, NO3-, SO42-, Na+, NH4+, K+, Mg2+ and Ca2+. Coulter counter technique was used for the dust measurements and the chemical analysis were carried out by ion chromatography....

  11. Root growth conditions in the topsoil as affected by tillage intensity

    DEFF Research Database (Denmark)

    Kadziene, Grazina; Munkholm, Lars Juhl; Mutegi, James


    treatments were direct drilling (D), harrowing 8–10 cm (H), and ploughing (P) to 20 cm depth. A chisel coulter drill was used in the H and D treatments and a traditional seed drill in the P treatment. Undisturbed soil cores were collected in November 2008 at soil field moisture capacity from the 4–8 and 12......Many studies have reported impeded root growth in topsoil under reduced tillage or direct drilling, but few have quantified the effects on the least limiting water range for root growth. This study explored the effects of tillage intensity on critical soil physical conditions for root growth in the...

  12. Fröplacering och vertikal rörelse för en fjädrande såbill på Väderstads rapidsåmaskin


    Karlsson, Johan


    For good crop emergence, the seed should be placed at a depth where good conditions concerning oxygen, temperature and water are provided. To place the seed in the soil, some type of see d coulter is used. Väderstad-Verkens Rapid is a seed drill that places the seed at a certain depth in relation to a wheel running on the ground. This can lead to a to shallow seed placement. On fields with clayey soils and spring sown cereals a to shallow seed placement can lead to poor emergence, especially ...

  13. Electrical determination of viability in saline-treated mouse myeloma cells.


    Matsushita, T.; Brendzel, A M; Shotola, M A; Groh, K R


    Suspension of mouse myeloma cells in phosphate buffered saline (PBS) induced a significant amount of cell death. The lethal effects of PBS include an increase in cell lysis, a decreased ability of cells to exclude trypan blue, and a decrease in the colony-forming ability of these cells. Dead cells were also detected on a Coulter counter by the increase in the fraction of cells with a smaller electrical size distribution (ESD). Comparing mixtures of live and dead cells by ESD and trypan-blue e...

  14. Size determinations of colloidal fat emulsions

    DEFF Research Database (Denmark)

    Kuntsche, Judith; Klaus, Katrin; Steiniger, Frank


    Size and size distributions of colloidal dispersions are of crucial importance for their performance and safety. In the present study, commercially available fat emulsions (Lipofundin N, Lipofundin MCT and Lipidem) were analyzed by photon correlation spectroscopy, laser diffraction with adequate...... slightly smaller size was indicated by all methods for Lipidem. Sub-micron resolution was best in the Coulter LS but the fraction of larger particles in the upper nm-range was presumably underestimated. The emulsions could be analyzed in a highly reproducible manner by asymmetrical flow field...

  15. Crystallization Thermodynamic and Kinetic Behaviors of Vitamin C in Batch Crystallizer

    Institute of Scientific and Technical Information of China (English)


    The bench-scale cooling crystallization for ternary solution of L-ascorbic acid (Vitamin C) was studied. The solid-liquid phase diagram of Vitamin C-water-ethanol system was obtained on the basis of differential scanning calorimeter (DSC) curves. The heat of crystallization of Vitamin C was calculated with the aid of quantitative analysis. According to the population balance equation under unsteady state, the rates of nucleation and growth were determined. The parameters of crystallization kinetics equations were estimated by regression of experimental data. Crystal morphology and size were determined with x-ray diffraction and TA Ⅱ Coulter Counter.

  16. Integrin β1, Osmosensing, and Chemoresistance in Mouse Ehrlich Carcinoma Cells

    DEFF Research Database (Denmark)

    Sørensen, Belinda Halling; Rasmussen, Line Jee Hartmann; Broberg, Bjørn Sindballe;


    RNA was used to silence integrin β1 expression. Regulatory volume decrease (RVD) after cell swelling was studied with calcein-fluorescence-self-quenching and Coulter counter analysis. Taurine efflux was estimated with tracer technique. Caspase assay was used to determine apoptosis. RESULTS: We show...... that adherent cells have stronger fibronectin binding and a significantly increased expression of integrin α5, αv, and β1 at mRNA and protein level, compared to non-adherent cells. Knockdown of integrin β1 reduced RVD of the adherent but not of the non-adherent cells. Efflux of taurine was unaffected...

  17. Validation of the Hemo_Control instrument for determination of B-haemoglobin concentration in primary health care

    DEFF Research Database (Denmark)

    Aakre, Kristin; Bliksrud, Yngve; Frøyshov, Bess; Hager, Helle; Hansen, Anne; Janukonyte, Jurgita; Lauterlein, Jens-Jacob; Lilleholt, Kristin; Løhne, Kari; Männistö, Tuija; Miettola, Satu; Pelanti, Jonna; Samson, Mie; Skauby, Ragnhild; Tuuti, Eija; Zelvyte, Inga


    at two primary health care centres. The bias of the Hemo_Control instrument was calculated as the difference between its results and results obtained with a Coulter LH 750 instrument traceable to the ICSH reference method. RESULTS: The uncertainty of the Hemo_Control instrument for venous samples was......_Control instrument showed negligible bias of +0.8 g/L for both venous and capillary samples in primary health care. CONCLUSION: The observed uncertainty indicates that Hemo_Control is appropriate for near patient testing using venous samples. Capillary samples may be used if sampling skills are adequate....

  18. Interstitial diffuse radiance spectroscopy of gold nanocages and nanorods in bulk muscle tissues


    Grabtchak S; Montgomery LG; Pang B; Wang Y; Zhang C; Li Z; Xia Y; Whelan WM


    Serge Grabtchak,1,2 Logan G Montgomery,1 Bo Pang,3,4 Yi Wang,4,5 Chao Zhang,6,7 Zhiyuan Li,6,7 Younan Xia,4,8 William M Whelan1,91Department of Physics, University of Prince Edward Island, Charlottetown, PEI, Canada; 2Departments of Electrical and Computer Engineering, and Physics, Dalhousie University, Halifax, Canada; 3Department of Biomedical Engineering, Peking University, Beijing, People’s Republic of China; 4The Wallace H Coulter Department of Biomedical Engineering, Georgia I...

  19. APSAS; an Automated Particle Size Analysis System (United States)

    Poppe, Lawrence J.; Eliason, A.H.; Fredericks, J.J.


    The Automated Particle Size Analysis System integrates a settling tube and an electroresistance multichannel particle-size analyzer (Coulter Counter) with a Pro-Comp/gg microcomputer and a Hewlett Packard 2100 MX(HP 2100 MX) minicomputer. This system and its associated software digitize the raw sediment grain-size data, combine the coarse- and fine-fraction data into complete grain-size distributions, perform method of moments and inclusive graphics statistics, verbally classify the sediment, generate histogram and cumulative frequency plots, and transfer the results into a data-retrieval system. This system saves time and labor and affords greater reliability, resolution, and reproducibility than conventional methods do.

  20. Cell type-dependent uptake, localization, and cytotoxicity of 1.9 nm gold nanoparticles


    Coulter JA; Jain S; Butterworth KT; Taggart LE; Dickson GR; McMahon SJ; Hyl; WB; Muir MF; Trainor C; Hounsell AR; O'Sullivan JM; Schettino G.; Currell FJ; Hirst DG


    Jonathan A Coulter,1 Suneil Jain,2 Karl T Butterworth,2 Laura Taggart,2 Glenn Dickson,2 Stephen J McMahon,3 Wendy Hyland,1 Mark F Muir,3 Coleman Trainor,2 Alan Hounsell,2,4 Joe M O'Sullivan,2,4 Giuseppe Schettino,2 Fred Currell,3 David G Hirst,1 Kevin M Prise21School of Pharmacy, McClay Research Centre, 2Centre for Cancer Research and Cell Biology, 3School of Mathematics and Physics, Queens University Belfast, 4Belfast Health and Social Care Trust, Belfast, IrelandBackground: This fol...

  1. A comparison of assays for the response of primary human T-cells upon stimulation with interleukin-2, interleukin-4 and interleukin-7


    Duschl, Albert; Jahn, Ute; Bertling, Claudia; Sebald, Walter


    The most commonly used assay to quantitate the response of peripheral T~cells upon stimulation with growth factors is determination of incorporated (JH]TdR. We compared thls test to three other methods: 1. direct countlog of cells with a Coulter type counter as reference assay, 2. a colorimetric assay using the tetrazolium dye 3-[ 4,S-dimethylthiazol-l-yl]-2,5diphenyl tetrazolium (MTT), which is a cheap and increasingly popular non-radioactive method and 3. incorporation of the thymidine anal...

  2. Particle size measurement in intravenous fluids. (United States)

    Haines-Nutt, R F; Munton, T J


    The level of particulate contamination in a range of large volume injections has been measured using electrical resistance (Coulter) and light blockage (HIAC) techniques. Particle counts showed large variations between the two techniques and although a correlation could be shown for ionic solutions no such correlation could be found for sugar containing solutions. Shape factors alone cannot explain these discrepancies but other differences fundamental to the physics of the two measuring techniques play an important part. We conclude that results obtained using one technique cannot be correlated, theoretically or actually, with those obtained from the other technique. PMID:6148396

  3. Size characterization of Mycobacterium bovis BCG (Bacillus Calmette Guérin) vaccine, Tice substrain. (United States)

    Zhang, A; Groves, M J


    Reconstituted, lyophilized, attenuated Mycobacterium bovis, Bacillus Calmette Guérin (BCG) vaccine, Tice substrain, was characterized using a Coulter Multisizer and a HIAC/Royco counter. The primary organism has an equivalent spherical diameter approximating 1 micron but the BCG cell suspension is heavily aggregated. The cumulative size distribution of the suspension fits a log-probit plot and this information can be used to determine the total number of particles per ampoule. The instrumental count may be related to the viable count. The state of dispersion was unaffected by mild shear (syringe aspiration or ultrasound) and only slightly affected by the addition of cetylpyridinium chloride or sodium tauroglycolate. PMID:3073388

  4. Analysis of images of acute human and animal leukaemia

    International Nuclear Information System (INIS)

    This research thesis first proposes a review of the development of stereology: historical backgrounds, basic principles. It discusses the choices regarding instrumentation: Coulter counter (principle and theory), quantitative analysis of particles, image analyser (optical microscope, epidiascope, scanners, detection, electronic pencil, computers, programming and data processing system), and stereo-logical parameters. The author then reports the stereo-logical study of acute human leukaemia: definition, classification, determination of spherical particle size distribution, lympho-blast size distributions. He reports the comparative study of rat L 5222 leukaemia and Brown Norway rat acute myelocytic leukaemia, and discusses their relationship with acute human leukaemia

  5. Trueness investigation of routine creatinine assays on nine homogeneous systems in Beijing demonstrates an encouraging outcome that meets clinical requirements

    Institute of Scientific and Technical Information of China (English)

    LIU Yan; XU Guo-bin


    Background Serum creatinine (Scr) measurement plays a key role in glomerular filtration rate estimation (eGFR),chronic kidney disease (CKD) diagnosis as well as CKD treatment. However, the test results of Scr from different laboratories vary significantly. In order to get comparable results, the European in vitro diagnostic (IVD) directive requires traceability to reference methods and materials. The purpose of this study was to verify the effect of traceability implementation by investigating the trueness of creatinine measurement on nine homogenous systems in Beijing. Methods Commutable frozen human serum reference material, National Institute of Standards & Technology (NIST) Standard Reference Material (SRM) 967, was used to verify the trueness of Scr measurement results from nine homogeneous analytical systems of seven companies which are the most widely used systems in Beijing's third-grade hospitals. The methods referred to the Jaffe's and Enzymatic methods. Results from nine routine measurement systems were assessed using two criteria: biological variability and Clinical Laboratory Improvement Amendments' 88 (CLIA' 88).We simulated a series of broken lines representing the limits of SD and bias that would produce a relative increase (or decrease) of 10% and 20% in the measurement error when estimating GFR (MEeGFR) using the isotope dilution mass spectrometry (IDMS)-traceable Modification of Diet in Renal Disease (MDRD) Study equation. Results of the College of American Pathologists (CAP) 2008-B LN24 Survey were compared with our investigation results. Results Compared with the total error criteria of biological variability, Ortho (traceable to IDMS) met the minimum acceptable criteria; Roche (Jaffe), Roche (Enzymatic), Shino and Daiichi met the desirable criteria at level Ⅰ. At level Ⅱ, Ortho (traceable to gas chromatography/isotope dilution mass spectrometry, GC/IDMS), Dade Behring and Beckman (traceable to rate Jaffe) met the minimum acceptable

  6. Field evaluation of slot openers for minimum tillage (part 3- Dried soil cover on the slot -

    Directory of Open Access Journals (Sweden)

    Isara Chaorakam


    Full Text Available The direction and feature of slot opener can produce different amount of dried soil cover on the slot. Four different slot openers, namely of a bubble coulter, a V-shaped slot opener, a VRA-slot opener and a VRB-slot opener were tested in the rice field which was covered with chopped arable weed. The direction of these four slot openers was set following the direction of travel. Several field trials of all slot openers were carried out to validate their drill performance on sandy loam soil with four different levels of residual density (0, 2, 4, 6 t/ha and three levels of soil moisture content (31.67, 46.34, 49.07 %. Significant interactions among the major factors were discussed for each slot opener. High amounts of dried soil cover on the slot were discerned in the case of a VRA-slot opener, especially under lower levels of soil moisture content. Some amount of dried soil cover on the slots was observed for the VRB-slot opener. Generally, dried soil cover on the slot tended to decrease with the increase of residual density after the operation of the bubble coulter and the V-shaped slot opener, due partly to hairpinned residue in their slots.

  7. Reaching out to the American middle class: Peggy Noonan’s conservatism

    Directory of Open Access Journals (Sweden)



    Full Text Available Parmi les idéologues qui popularisent les idées conservatrices auprès du grand public, Peggy Noonan occupe une place intéressante. Moins extrémiste que Rush Limbaugh ou Ann Coulter, elle exprime les angoisses et les espoirs des Reagan Democrats, ces Américains moyens devenus conservateurs depuis les années 80. Cet article analyse les ambivalences d’un discours qui entre en résonance avec celles d’une partie de la classe moyenne américaine.Since the 1990s, Peggy Noonan’s writings have translated conservative ideas into articles and bestselling books for the wider American public. In a less abrasive way than Rush Limbaugh or Ann Coulter, she expresses the hopes and fears of the Reagan Democrats. This article analyzes Noonan’s ambivalent rhetoric, which mirrors the ambivalent attitudes of parts of America’s embattled middle class.

  8. An evaluation of the ELT-8 hematology analyzer. (United States)

    Raik, E; McPherson, J; Barton, L; Hewitt, B S; Powell, E G; Gordon, S


    The TMELT-8 Hematology Analyzer is a fully automated cell counter which utilizes laser light scattering and hydrodynamic focusing to provide an 8 parameter whole blood count. The instrument consists of a sample handler with ticket printer, and a data handler with visual display unit, It accepts 100 microliter samples of venous or capillary blood and prints the values for WCC, RCC, Hb, Hct, MCV, MCH, MCHC and platelet count on to a standard result card. All operational and quality control functions, including graphic display of relative cell size distribution, can be obtained from the visual display unit and can also be printed as a permanent record if required. In a limited evaluation of the ELT-8, precision, linearity, accuracy, lack of sample carry-over and user acceptance were excellent. Reproducible values were obtained for all parameters after overnight storage of samples. Reagent usage and running costs were lower than for the Coulter S and the Coulter S Plus. The ease of processing capillary samples was considered to be a major advantage. The histograms served to alert the operator to a number of abnormalities, some of which were clinically significant. PMID:7099723

  9. Resolution of two surface glycoproteins from human parainfluenza-3 virus by crossed immunoelectrophoresis. (United States)

    Holling, R A; Guskey, L E


    The technique of two-dimensional crossed immunoelectrophoresis (CIE) was used to resolve two glycoproteins from purified human parainfluenza type 3 virus. Virus preparations were extracted with Triton X-100 and fractionated by centrifugation in a Beckman airfuge. Two immunoprecipitates were detected by CIE in the supernatant fractions, but were not found in the pellets from extracted virus. Viral glycoproteins labeled with [35S]methionine were isolated by affinity chromatography on concanavalin A (Con A) agarose columns, resolved by CIE and detected by autoradiography. Resolution of two glycoprotein peaks from as little as 4.5 micrograms of protein from extracted virus is consistent with results from polyacrylamide gel patterns showing two unique glycoproteins with molecular weights of 48 kd and 65 kd. PMID:6088566

  10. Reproducing sterile neutrinos and the behavior of flavor oscillations with superconducting-magnetic proximity effects (United States)

    Baker, Thomas E.


    The physics of a superconductor subjected to a magnetic field is known to be equivalent to neutrino oscillations. Examining the properties of singlet-triplet oscillations in the magnetic field, a sterile neutrino is suggested to be represented by singlet Cooper pairs and moderates flavor oscillations between three flavor neutrinos (triplet Cooper pairs). A superconductor-exchange spring system's rotating magnetization profile is used to simulate the mass-flavor oscillations in the neutrino case and the physics of neutrino oscillations are discussed. Connecting the condensed matter system and the particle physics system with this analogy may allow for the properties of the condensed matter system to inform neutrino experiments. Support is graciously acknowledged from the Pat Beckman Memorial Scholarship from the Orange County Chapter of the Achievement Rewards for College Scientists Foundation.

  11. Limb blood flow - Rest and heavy exercise in sitting and supine positions in man (United States)

    Greenleaf, J. E.; Montgomery, L. D.; Brock, P. J.; Van Beaumont, W.


    The objectives of the study were twofold: (1) to determine the effect of body position (hydrostatic pressure) on total blood flow in active and passive limbs at rest and after severe exercise; and (2) to further evaluate the impedance technique for measurement of blood flow. To this end, the effect of body position on the redistribution of total blood flow in active (leg) and passive (forearm) limbs during exercise was determined by measuring total limb impedance (blood flow) in five male and one female subjects in sitting and supine positions with a modified Beckman BR-100 rheograph. The results show that, at rest and after exercise, the supine position induces significantly greater flows in the leg but not in the forearm. With severe exercise, blood flows are increased in both passive and active limbs, so that there is probably no net transfer of blood volume from passive to active muscles. The advantages of the impedance technique over other methods are stressed.

  12. Exploring variation in accuracy and contrast for sibilant fricatives at the onset of fricative acquisition (United States)

    Nicholson, Hannele Buffy Marie

    Children's speech differs from adult speech in the many ways, including in its phonetic characteristics. A central question for researchers interested in child speech sound acquisition is when and how a child acquires robust adult-like contrasts. In this thesis, I present a protocol for the analysis of the English sibilant fricatives "s" and "sh". Sibilant fricatives are of interest because they are late-acquired sounds that require articulatory-aerodynamic coordination, and are contrastively necessary in multiple languages around the world, English especially. Given the turbulent nature of the sound spectrum of fricative consonants, few agreed upon measures exist. Holliday, Reidy, Beckman and Edwards (In Preparation) propose that peak equivalent rectangular bandwidth is a psychoacoustically appropriate measure for modeling the robustness of phonological contrast between sibilant fricative types. The robustness measures put forth by Holliday et al. are applied to data from the speech of toddlers aged 28-39 months and are discussed.

  13. 三种CA125检测系统的一致性评价%Measuring agreement in comparison of CA125 determined by three different immunoassay systems

    Institute of Scientific and Technical Information of China (English)

    张巧娣; 谢而付; 张燕; 凌芸; 蒋理; 徐华国


    Objective:To assess the agreement of three different chemiluminescent detection system of CA125 measurement by using Bland-Altman analysis.Methods:One hundreds serum samples were obtained from 100 patients. CA125 of these samples were measured by three different chemiluminescent detection system, including Roche, Beckman and Siemens. Bland-Altman analysis and Spearman correlation analysis were performed to assess the results of every two systems, and draw the limits of agreement as an evaluation of agreement of different systems. Results:The distribution of CA125 results of Roche, Beckman and Siemens system were 5.6~279.8, 4.5~542.4 and 4.5~350.0 U/mL, respectively. Spearman correlation analysis showed that the results of every two methods had good correlation, the correlation coeffcient were 0.932 (P=0.000) between Roche and Beckman, 0.947 (P=0.000) between Roche and Siemens, 0.948 (P=0.000) between Beckman and Siemens. Bland-Altman analysis for the measurements showed bad relativity. The Bland-Altman plot revealed lower agreement between every two methods for CA125 results.Conclusion:Three detection systems do not have the consistency of the CA125 results, it is recommended to establish a standardized reference system of CA125 for comparison.%目的:使用Bland-Altman分析法,评估三种化学发光检测系统测定血清糖链抗原125(cancerantigen,CA125)结果之间的一致性.方法:选取100例患血清,分别使用Roche、Beckman和Siemens公司的检测系统进行CA125检测,对每两种检测系统之间的检测结果进行相关性分析与Bland-Altman分析,并绘制一致性限度作为评价一致性的指标.结果:Roche、Beckman和Siemens检测系统检测CA125结果范围分别为5.6~279.8、4.5~542.4和4.5~350.0U/mL;不同检测系统间相关系数如下:Roche与Beckman为0.932(P=0.000),Roche与Siemens为0.947(P=0.000),Beckman与Siemens为0.948(P=0.000),每两组之间均存在相关;Bland-Altman一致性

  14. A High-affinity Activator of G551D-CFTR Chloride Channel Identified By High Throughput Screening

    Institute of Scientific and Technical Information of China (English)

    ZHAO Lu; HE Cheng-yan; LIU Yan-li; ZHOU Hong-lan; ZHOU Jin-song; SHANG De-jing; YANG Hong


    A stably transfected CHO cell line coexpressing G551D-CFTR and iodide-sensitive yellow fluorescent protein mutant EYFP-H148Q-I152L was successfully established and used as assay model to identify small-molecule activators of G551D-CFTR chloride channel from 100000 diverse combinatorial compounds by high throughput screening on a customized Beckman robotic system. A bicyclooctane compound was identified to activate G551D-CFTR chloride channel with high-affinity(Kd=1.8 μmol/L). The activity of the bicyclooctane compound is G551D-CFTR-specific, reversible and non-toxic. The G551D-CFTR activator may be useful as a tool to study the mutant G551D-CFTR chloride channel structure and transport properties and as a candidate drug to cure cystic fibrosis caused by G551D-CFTR mutation.


    Institute of Scientific and Technical Information of China (English)

    朱寿彭; 王六一


    For labelling spermatid DNA,male BALB/c mice are injected intratesticularyly with 3H-TdR in advance.After 36d of labelling,spermatozoa are sampled which is just the time required for the germ cells to mature.The period from intraperitoneal injection of 147Pm to sampling spermatozoa is 12d.After sperm cells have been lysed.30ml eluent is added by wriggle pump.Finally,both the DNA remained on the filters and filtrated bottles are determined with a Beckman liquid scintillation device.Results show that after small dose internal irradiation with 185Bq/g of 147Pm,an increase in amount of sperm DNA on the filter observed is considerably higher than the control group.This indicates that low level internal radiation of 147Pm has tendency to stimulate the DNA repair in spermiogenic stages.

  16. Applicability of a prototype for determination of absorbed dose using brachytherapy equipment with Ir-192 sources; Aplicabilidade de um prototipo para determinacao da dose absorvida utilizando equipamentos de braquiterapia com fontes de IR-192

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Vivianne Lucia Bormann; Almeida, Mayara Gabriella Oliveira de; Vieira, Rafaela Etelvina de Amorim; Silva, Waldecy Ananias da; Nascimento, Rizia Keila, E-mail:, E-mail:, E-mail:, E-mail:, E-mail: [Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil)


    This work aims at the development and improvement of a device to perform the absolute dosimetry sources of Ir-192 using the Fricke solution contained in a flask. The Fricke solution used was prepared using amounts of ferrous ammonium sulfate, sodium chloride and sulfuric acid, diluted with water tri distilled pre-established in the literature. The spectrophotometer used was a UV-VIS spectrophotometer (Beckman DU-640 Counter) for measuring the optical density at wavelength 304 nm. The calculation for determining the radial dose takes into account the radial distance and the angle formed with the transverse axis of the source. As the results obtained can be seen that the states of Pernambuco, Ceara, Paraiba e Piaui are in accordance with the recommendations of international standards of the International Atomic Energy Agency (IAEA), which considers not acceptable a difference greater than 5% of prescribed dose and measured dose.

  17. Comparison of blood glucose test results between point-of-care testing glucose meter and biochemical analyzer%POCT血糖仪与生化分析仪血糖检测结果的比对试验及分析

    Institute of Scientific and Technical Information of China (English)



    Objective To explore precision and accuracy of point-of-care testing(POCT) blood glucose meter through comparing blood glucose results between POCT glucose meter and biochemical analyzer. Methods Selected 4 sets of POCT glucose meter to detect the same sample and compared blood glucose results between Beckman CX-9 biochemical analyzer and POCT blood glucose meter. Results Measured results of Beckman CX-9 biochemical analyzer and POCT glucose meter were highly correlated(P>0.05), differences were not statistically significant. Conclusion POCT blood glucose meter is a consistent and convenient detection method for measuring blood glucose results as biochemical analyzer, so it is worthy of clinical promotion and application.%目的对比床旁检测(POCT)血糖仪与生化分析仪对血糖测定的结果,对POCT血糖仪的精密度与准确度进行探讨。方法选用4台POCT血糖仪对同一标本进行测定,并比较贝克曼CX-9生化分析仪与POCT血糖仪的测定结果。结果贝克曼CX-9生化分析仪与POCT血糖仪的测定结果高度相关(P>0.05),差异不具有统计学意义。结论 POCT血糖仪与生化分析仪对血糖的测定结果具有一致性,且方便快捷,可作为急诊的检测方法,值得在临床过程中推广、应用。

  18. El liderazgo docente y su relación con el estilo gerencial de los pasantes de Comunicación Social de la Universidad del Zulia

    Directory of Open Access Journals (Sweden)

    Rosario Fonseca


    Full Text Available Se propuso determinar la relación entre el liderazgo docente y el estilo gerencial de los estudiantes de Comunicación Social, mención Periodismo Impreso de LUZ, de acuerdo a la clasificación de Robbins, Stephen y Coulter (1999, y Guédez (1996 y siguiendo una metodología descriptiva-correlacional. Se aplicó un cuestionario para docentes y estudiantes de los últimos semestres. Los resultados demuestran que existe relación entre el liderazgo de los docentes, en su mayoría de tipo Democrático-Situacional, y el estilo gerencial de los estudiantes, predominó el estilo Estratégico y Situacional.

  19. [Studies on the stability and some other important physical properties of polysaccharides polyphase liposome (PPL)]. (United States)

    Zou, Y Y; Su, D S; Han, G C; Gu, X Q


    The stability and some other important physical properties of PPL were investigated by means of electron microscopy and MPV-TAS experiments. The results are rated as follows: Good integrity of liposome homogeneity of its particle size were reached, and at 100 degrees C PPL was found to undergo sterilization without causing changes of its shape and encapsulation efficiency for 1 h. Also, the distribution of PPL particle size and the regularity of the change of PPL particle in size were determined by Coulter counter. Using this method, the kinetic equation of coalescence of polyphase liposome and the activation energy for coalescence were obtained, and through these results the stability of PPL is elucidated. PMID:2508432

  20. Survival and activity of Streptococcus faecalis and escherichia coli in tropical freshwater

    International Nuclear Information System (INIS)

    The survival of Streptococcus faecalis and Escherichia coli was studied in situ in a tropical rain forest watershed using membrane diffusion chambers. Densities were determined by acridine orange direct count and Coulter Counter. Population activity was determined by microautoradiography, cell respiration, and by nucleic acid composition. Densities of S. faecalis and E. coli decreased less than 1 log unit after 105 hours as measured by direct count methods. Activity as measured by respiration, acridine orange activity, and microautoradiography indicated that both bacteria remained moderately active during the entire study. After 12 hours, E. coli was more active than S. faecalis as measured by nucleic acid composition. In this tropical rain forest watershed, E. coli and S. faecalis survived and remained active for more than 5 days; consequently, both would seem to be unsuitable as indicators of recent fecal contamination in tropical waters

  1. Cell counting. (United States)

    Phelan, M C; Lawler, G


    This unit presents protocols for counting cells using either a hemacytometer or electronically using a Coulter counter. Cell counting with a hemacytometer permits effective discrimination of live from dead cells using trypan blue exclusion. In addition, the procedure is less subject to errors arising from cell clumping or size heterogeneity. Counting cells is more quickly and easily performed using an electronic counter, but live-dead discrimination is unreliable. Cell populations containing large numbers of dead cells and/or cell clumps are difficult to count accurately. In addition, electronic counting requires resetting of the instrument for cell populations of different sizes; heterogeneous populations can give rise to inaccurate counts, and resting and activated cells may require counting at separate settings. In general, electronic cell counting is best performed on fresh peripheral blood cells. PMID:18770655

  2. Survival and activity of Streptococcus faecalis and Escherichia coli in tropical freshwater

    Energy Technology Data Exchange (ETDEWEB)

    Muniz, I.; Jimenez, L.; Toranzos, G.A.; Hazen, T.C. [Univ. of Puerto Rico, Rio Piedras (Puerto Rico)


    The survival of Streptococcus facecalis and Escherichia coli was studied in situ in a tropical rain forest watershed using membrane diffusion chambers. Densities were determined by acridine orange direct count and Coulter Counter. Population activity was determined by microautoradiography, cell respiration, and by nucleic acid composition. Densities of S. facecalis and E. coli decreased less than 1 log unit after 105 h as measured by direct count methods. Activity as measured by respiration, acridine orange activity, and microautoradiography indicated that both bacteria remained moderately active during the entire study. After 12 h, E. coli was more active than S. faecalis as measured by nucleic acid composition. E. coli and S. faecalis survived and remained active for more than 5 days. Consequently, both would seem to be unsuitable as indicators of recent fecal contamination in tropical waters.

  3. Efficiency of dust sampling inlets in calm air. (United States)

    Breslin, J A; Stein, R L


    Measurement of airborne dust concentrations usually involves drawing a sample of the dust-laden air into the measuring instrument through an inlet. Even if the surrounding air is calm, theoretical calculations predict that large particles may not be sampled accurately due to the combined effects of gravity and inertia on the particles near the sampling inlet. Tests were conducted to determine the conditions of particle size, inlet radius, and flow rare necessary for accurate dust sampling. A coal-dust aerosol was sampled simultaneously through inlets of different diameters at the same volume flow-rate and collected on filters. The dust was removed from the filters and the particles were counted and sized with a Coulter counter. Results showed that published criteria for inlet conditions for correct sampling are overly restrictive and that respirable-size particles are sampled correctly in the normal range or operation of most dust sampling instruments. PMID:1227283

  4. Visible and subvisible particles in the BCG immunotherapeutic product Immucyst® (United States)

    Kirkitadze, Marina; Remi, Elena; Bhandal, Kamajit; Carpick, Bruce


    Bacille Calmette–Guerin, BCG, is a live attenuated bovine tubercle bacillus used for the treatment of non-muscle invasive bladder cancer. In this study, an Electrical Sensing Zone (ESZ) method was developed to measure the particle count and the size of BCG immunotherapeutic (BCG IT), or ImmuCyst® product using a Coulter Counter Multisizer 4® instrument. The focus of this study was to establish a baseline for reconstituted lyophilized BCG IT product using visible and sub-visible particle concentration and size distribution as reportable values. ESZ method was used to assess manufacturing process consistency using 20 production scale lots of BCG IT product. The results demonstrated that ESZ can be used to accumulate product and process knowledge of BCG IT. PMID:27158432

  5. Photon correlation spectroscopy of classical and non-classical light fields and its debt to Glauber and Harvard

    International Nuclear Information System (INIS)

    The Union Gikon Company of Japan has designed their new photon-correlation spectrometer to the outside world with the aid of a number of Japanese Universities and Industrial Research Laboratories. It comes with a list of some two dozen Japanese Institutions who have been using it successfully since its recent launch in their home country. The cost of the system is some $60,000 and it will no doubt, find its place in the market alongside existing well-known photon correlation systems such as those from Brookhaven Instruments Corporation, Hiac Royco, Coulter and Malvern Instruments. Although they may be used for other purposes, the main application of the instruments is the measurement of sizes of submicron particles such as proteins, enzymes, viruses, polymers and numerous other macro-molecular substances. A topical proposal, for example, is the detection of the immunological reactions of the AIDS virus. Photon correlation spectroscopy has become an important technique in modern laboratory practice

  6. Ultra-Fast Low Concentration Detection of Candida Pathogens Utilizing High Resolution Micropore Chips

    Directory of Open Access Journals (Sweden)


    Full Text Available Although Candida species are the fourth most common cause of nosocomial blood stream infections in the United States, early diagnostic tools for invasive candidemia are lacking. Due to an increasing rate of candidemia, a new screening system is needed to detect the Candida species in a timely manner. Here we describe a novel method of detection using a solid-state micro-scale pore similar to the operational principles of a Coulter counter. With a steady electrolyte current flowing through the pore, measurements are taken of changes in the current corresponding to the shape of individual yeasts as they translocate or travel through the pore. The direct ultra-fast low concentration electrical addressing of C. albicans has established criteria for distinguishing individual yeast based on their structural properties, which may reduce the currently used methods’ complexity for both identification and quantification capabilities in mixed blood samples

  7. Visible and subvisible particles in the BCG immunotherapeutic product Immucyst®. (United States)

    Kirkitadze, Marina; Remi, Elena; Bhandal, Kamajit; Carpick, Bruce


    Bacille Calmette-Guerin, BCG, is a live attenuated bovine tubercle bacillus used for the treatment of non-muscle invasive bladder cancer. In this study, an Electrical Sensing Zone (ESZ) method was developed to measure the particle count and the size of BCG immunotherapeutic (BCG IT), or ImmuCyst® product using a Coulter Counter Multisizer 4® instrument. The focus of this study was to establish a baseline for reconstituted lyophilized BCG IT product using visible and sub-visible particle concentration and size distribution as reportable values. ESZ method was used to assess manufacturing process consistency using 20 production scale lots of BCG IT product. The results demonstrated that ESZ can be used to accumulate product and process knowledge of BCG IT. PMID:27158432

  8. Crop establishment of Silphium perfoliatum by precision seeding

    Directory of Open Access Journals (Sweden)

    Andreas Schäfer


    Full Text Available Silphium perfoliatum, a perennial composite from North America, has been found as a promising plant species during the search for alternative biogas substrates. Until now Silphium perfoliatum has been established by the time- and cost-intensive transplanting method. The precision sowing of Silphium perfoliatum reduces the process costs significantly. However, the bulky seed with mildness germination power complicates a well distributed and adequate plant establishment. In field tests a modified precision seeder was used for sowing Silphium perfoliatum which is primarily used for maize sowing.The hole diameter of the singling disc, the sowing coulter and the roller were modified. In the field tests the modified machinery proved an enhanced and uniform field emergence of Silphium perfoliatum.

  9. Investigations of heavy ion tracks in polyethylene naphthalate films

    CERN Document Server

    Starosta, W; Sartowska, B; Buczkowski, M


    The heavy ion beam (with fluence 3x10 sup 8 ion/cm sup 2) from a cyclotron has been used for irradiation of thin polyethylene naphthalate (PEN) films. Latent tracks in these polymeric films have been sensitized by UV radiation and then chemically etched in NaOH solution. The etching process parameters have been controlled by the electroconductivity method. After etching, parameters of samples have been examined by SEM and bubble point methods (Coulter[reg] Porometer II instrument). Results have shown good quality of PEN track membranes with pore sizes in the range: 0.1 - 0.5 mu m. The described procedure is known for thin polyethylene terephthalate (PET) films. Taking into consideration that PEN films have got better mechanical, thermal, gas barrier as well as better chemical resistance properties in comparison with PET films, the possibility of application of such membranes is much wider.

  10. Red blood cell sorting with a multi-bed microfabricated filter

    International Nuclear Information System (INIS)

    A microfabricated fluidic chip for sorting red blood cells (RBCs) by size has been designed, fabricated and tested. The performance of the chip has been compared against a flow cytometer using samples from identical populations of cells, and statistically significant (p < 0.0005) differences in the measured cell size distributions were observed. The measurement paradigm reported here differs from previously demonstrated devices such as microfabricated Coulter counters or flow cytometers, in that the analysis is inherently parallel and is thus suitable for high throughput, point-of-care analysis. This study is empirical and semi-quantitative. However, important features of RBC trapping are characterized and indications for improved device design are described. (paper)

  11. Experimental determination of multiple thermodynamic and kinetic risk factors for nephrolithiasis in the urine of healthy controls and calcium oxalate stone formers: does a universal discriminator exist? (United States)

    Rodgers, A L; Webber, D; Hibberd, B


    Nephrolithiasis is thought to be governed by urinary thermodynamic and kinetic risk factors. However, identification of one or more of these factors which consistently and unambiguously differentiates between healthy subjects (N) and calcium oxalate (CaOx) renal stone patients (SF) remains elusive. The present study addresses this challenge. 24 h urines were collected from 15 N and 10 SF. Urine compositions were used to compute thermodynamic risk indices including urinary ratios, quotients and supersaturation (SS) values, while CaOx metastable limits (MSL) were determined experimentally. Crystallisation kinetics was determined by measuring rates of particle formation (number, volume, size) using a Coulter counter multisizer (CC) and a Coulter flow cytometer (FC). Particle shapes were qualitatively differentiated by FC and were viewed directly by scanning electron microscopy. Several urinary composition ratios and risk quotients were significantly different between the groups. However, there were no significant differences between CaOx MSL or SS values. Using transformed FC data, the rate of CaOx crystallisation in SF was significantly greater than in N. This was not supported by CC measurements. There were no significant differences between the groups with respect to particle size or CaOx crystal growth rates. Single and aggregated CaOx dihydrate crystals were observed in both groups with equal frequency and there were no differences in the kinetic properties of these deposits. A few CaOx monohydrate crystals were observed in SF. Although several risk factors were found to be significantly different between the groups, none of them were consistently robust when compared to other cognate factors. Arguments were readily invoked which demonstrated inter-factor inconsistencies and conflicts. We suspect that a unique discriminatory factor, such as any of those which we investigated in the present study, may not exist. PMID:26198547

  12. Influence of icterus on determination of serum inorganic phosphorous by phosphomolybdate method%黄疸对磷钼酸法测血清无机磷的影响

    Institute of Scientific and Technical Information of China (English)

    钟虹; 梁美春; 包国祥


    目的:探讨黄疸对磷钼酸法测血清无机磷的影响.方法:选择293例黄疸标本,根据《诊断学》标准,按黄疸程度分为轻度黄疸(含总胆红素34μmoL/L~ 170 μmol/L),中度黄疸(含总胆红素171μmoI/L~340 μmoL/L),重度黄疸(含总胆红素>340 μmoL/L),黄疸标本分别在雅培AEROSET和贝克曼LX20两台生化仪上分别进行血清无机磷测定,所测结果进行统计学分析.结果:不同程度的黄疸标本在两台全自动生化仪上所测结果比较均有明显差别(P均为0,P<0.01),且均有良好的相关性.结论:黄疸标本在雅培组影响血清无机磷的测定,所测结果低于贝克曼组,并随黄疸程度的加深,测定结果偏低的趋势有加大的倾向.%Objective:To evaluate the influence of icterus on the determination of serum inorganic phosphorous by phosphomolybdate method. Methods: Two hundred and ninety - three patients were classified into mild icterus (Total bilirubin 34 μmoL/L ~ 170 μmoL/L), medium icterus(Total bilirubin 171 μmoL/L ~340 μmoL/L), serious icterus (Total bilirubin > 340 μmoL/L) according to the "Diagnostics". Two kinds of automatic biochemistry analyzers (Abbott Aeroset and Beckman LX20) were employed to measure the serum inorganic phosphorous in different degrees icterus. All results were analyzed statistically. Results: The measured values obtained by the two kinds of automatic biochemistry analyzers had significant difference. And they have good correlation. Conclusion.- With Abbott Aeroset, the measured values of the serum inorganic phosphorous are influenced by the icterus. The measured values are below those by Beckman. And with the deepening of icterus degree, the measured values showed a decrease trend.

  13. Smoking cessation: an application of theory of planned behavior to understanding progress through stages of change. (United States)

    Bledsoe, Linda K


    The purpose of this research was to investigate variables relevant to smoking cessation early in the process of change through an application of the Theory of Planned Behavior [Ajzen, I. (1985). From intentions to actions: A theory of planned behavior. In J. Kuhl and J. Beckman (Eds). Action-control: From cognition to behavior (pp.11-39). Heidelberg: Springer.] to the temporal structure provided by the Transtheoretical Model. Study 1 was a preliminary elicitation study (n=68) conducted to ground the concepts used in the model testing in Study 2 [Ajzen, I., Fishbein, M. (1980). Understanding attitudes and predicting social behavior, Englewood Cliffs, NJ: Prentice-Hall.]. Study 2 tested the proposed model fit with data from a sample of 230 adult smokers. Structural equation modeling did not support the Theory of Planned Behavior as a model of motivation for progress through the stages of change and highlighted measurement issues with perceived behavioral control. A modified model using the Theory of Reasoned Action provided a good fit to the data, accounting for approximately 64% of the variance in intention to quit smoking and stage of change. This research addresses the need for a more complete theoretical rationale for progress through stages of change. PMID:16182458

  14. Cytotoxic effect of Erythroxylum suberosum combined with radiotherapy in head and neck cancer cell lines

    International Nuclear Information System (INIS)

    The mouth and oropharynx cancer is the 6th most common type of cancer in the world. The treatment may involve surgery, chemotherapy and radiotherapy. More than 50% of drugs against cancer were isolated from natural sources, such as Catharanthus roseus and epipodophyllotoxin, isolated from Podophyllum. The biggest challenge is to maximize the control of the disease, while minimizing morbidity and toxicity to the surrounding normal tissues. The Erythroxylum suberosum is a common plant in the Brazilian Cerrado biome and is popularly known as 'cabelo-de-negro'. The objective of this study was to evaluate the cytotoxic activity of Erythroxylum suberosum plant extracts of the Brazilian Cerrado biome associated with radiotherapy in human cell lines of oral and hypopharynx carcinomas. Cells were treated with aqueous, ethanolic and hexanic extracts of Erythroxylum suberosum and irradiated at 4 Gy, 6 Gy and 8 Gy. Cytotoxicity was evaluated by MTT assay and the absorbance was measured at 570 nm in a Beckman Counter reader. Cisplatin, standard chemotherapy, was used as positive control. The use of Erythroxylum suberosum extracts showed a possible radiosensitizing effect in vitro for head and neck cancer. The cytotoxicity effect in the cell lines was not selective and it is very similar to the effect of standard chemotherapy. The aqueous extract of Erythroxylum suberosum, combined with radiotherapy was the most cytotoxic extract to oral and hypopharynx carcinomas. (author)

  15. The determination of calcium in phosphate, carbonate, and silicate rocks by flame photometer (United States)

    Kramer, Henry


    A method has been developed for the determination of calcium in phosphate, carbonate, and silicate rocks using the Beckman flame photometer, with photomultiplier attachement. The sample is dissolved in hydrofluoric, nitric, and perchloric acids, the hydrofluoric and nitric acids are expelled, a radiation buffer consisting of aluminum, magnesium, iron, sodium, potassium, phosphoric acid, and nitric acid is added, and the solution is atomized in an oxy-hydrogen flame with an instrument setting of 554 mµ. Measurements are made by comparison against calcium standards, prepared in the same manner, in the 0 to 50 ppm range. The suppression of calcium emission by aluminum and phosphate was overcome by the addition of a large excess of magnesium. This addition almost completely restores the standard curve obtained from a solution of calcium nitrate. Interference was noted when the iron concentration in the aspirated solution (including the iron from the buffer) exceeded 100 ppm iron. Other common rock-forming elements did not interfere. The results obtained by this procedure are within ± 2 percent of the calcium oxide values obtained by other methods in the range 1 to 95 percent calcium oxide. In the 0 to 1 percent calcium oxide range the method compares favorably with standard methods.

  16. Cytotoxic effect of Erythroxylum suberosum combined with radiotherapy in head and neck cancer cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Macedo, Taysa B.C.; Torres, Hianne M.; Yamamoto-Silva, Fernanda Paula; Silva, Maria Alves G. [Universidade Federal de Goias (UFG), Goiania, GO (Brazil). Escola de Odontologia; Elias, Silvia T.; Silveira, Damaris; Magalhaes, Perola O.; Lofrano-Porto, Adriana; Guerra, Eliete N.S., E-mail: [Universidade de Brasilia (UnB), Brasilia, DF (Brazil). Faculdade de Ciencias da Saude


    The mouth and oropharynx cancer is the 6{sup th} most common type of cancer in the world. The treatment may involve surgery, chemotherapy and radiotherapy. More than 50% of drugs against cancer were isolated from natural sources, such as Catharanthus roseus and epipodophyllotoxin, isolated from Podophyllum. The biggest challenge is to maximize the control of the disease, while minimizing morbidity and toxicity to the surrounding normal tissues. The Erythroxylum suberosum is a common plant in the Brazilian Cerrado biome and is popularly known as 'cabelo-de-negro'. The objective of this study was to evaluate the cytotoxic activity of Erythroxylum suberosum plant extracts of the Brazilian Cerrado biome associated with radiotherapy in human cell lines of oral and hypopharynx carcinomas. Cells were treated with aqueous, ethanolic and hexanic extracts of Erythroxylum suberosum and irradiated at 4 Gy, 6 Gy and 8 Gy. Cytotoxicity was evaluated by MTT assay and the absorbance was measured at 570 nm in a Beckman Counter reader. Cisplatin, standard chemotherapy, was used as positive control. The use of Erythroxylum suberosum extracts showed a possible radiosensitizing effect in vitro for head and neck cancer. The cytotoxicity effect in the cell lines was not selective and it is very similar to the effect of standard chemotherapy. The aqueous extract of Erythroxylum suberosum, combined with radiotherapy was the most cytotoxic extract to oral and hypopharynx carcinomas. (author)

  17. Biological characterization of a novel and potent gonadotropin releasing hormone (GnRH) antagonist LXT-101

    Institute of Scientific and Technical Information of China (English)

    Xiao-liCHI; Wen-xiaZHOU; Jun-pingCHENG; Yong-xiangZHANG; Ke-liangLIU


    AIM The biological characterization of LXT-101 was investigated in vivo using intact male rats and nude mice bearing xenografts of LNCaP prostate cancer. The effect of LXT-101 on the proliferation of androgen -sensitive prostate cancer cell LNCaP and androgen-insensitive DU145 and PC-3M in vitro was also determined. METHODS Rats were injected subcutaneously with LXT-101 while control animals received only vehicle (5% mannitol). Blood samples were collected at different time after adminis-tration of LXT-101. The androgen-dependent LNCaP prostate cancer cells were grown, mixed with Matrigel and injected subcutaneously into nude mice. Experimental group received LXT-101 injectionfor up to 4 weeks. At the end point, blood samples were drawn and the excised tumors and sex organswere weighted. The serum testosterone was determined by specific immunochemiluminescence assay using kits produced by Beckman-Counter Co. The mRNA expressions of the genes of hormone receptor related to the gonadal axis were investigated by real-time RT-PCR technique. Cell viability was determined by MTT method.

  18. Platelet count estimation using the CellaVision DM96 system

    Directory of Open Access Journals (Sweden)

    Yuon Gao


    Full Text Available Introduction: Rapid and accurate determination of platelet count is an important factor in diagnostic medicine. Traditional microscopic methods are labor intensive with variable results and are highly dependent on the individual training. Recent developments in automated peripheral blood differentials using a computerized system have shown many advantages as a viable alternative. The purpose of this paper was to determine the reliability and accuracy of the CellaVision DM 96 system with regards to platelet counts. Materials and Methods: One hundred twenty seven peripheral blood smears were analyzed for platelet count by manual microscopy, an automated hematology analyzer (Beckman Counter LH 780 or Unicel DXH 800 analyzers and with the CellaVision DM96 system. Results were compared using the correlations and Bland-Altman plots. Results: Platelet counts from the DM96 system showed an R 2 of 0.94 when compared to manual platelet estimates and an R 2 of 0.92 when compared to the automated hematology analyzer results. Bland-Altman plots did not show any systematic bias.

  19. Validation of the HTO-18 method for determination of CO2 production of lizards (genus Sceloporus)

    International Nuclear Information System (INIS)

    The accuracy of doubly-labeled water measurements of CO2 production in lizards of the genus Sceloporus was assessed by comparison of CO2 production rates determined simultaneously by labeled water and gas chromatography. Five lizards were weighed and given intraperitoneal injections of 55 μl of water containing 10 microcuries of tritium as HTO and 50 atom % oxygen-18 as H218O. Initial blood samples were taken from the infraorbital sinus ten hours later, and the lizards were placed in sealed metabolism chambers kept at 28 C. After 179 h the lizards were weighed and blood samples taken. Blood samples were microdistilled, assayed for tritium activity and for oxygen-18 content. Isotope measurements were used to calculate rates of CO2 production. Gas samples were withdrawn from each chamber after 18, 63, 109, and 179 h and measured against 0.5 and 1.0% CO2 standards with a Beckman GC-55 gas chromatograph fitted with silica gel 42-60 mesh column. These results were used to calculate rates of CO2 production. Results supported the conclusion that the doubly-labeled water method accurately measured rates of CO2 production in Sceloporus lizards, and could therefore be a valuable technique in field studies of lizard energetics

  20. 固相萃取-反相高效液相色谱法同时测定人血清中地西泮及氯硝西泮%Simultaneous determination of diazepam and clonazepam in human serum by solid phase extraction RP-HPLC

    Institute of Scientific and Technical Information of China (English)

    张吟; 陈一农; 郑兴中


    目的:建立一种快速、灵敏的同时测定人血清中地西泮与氯硝西泮的HPLC方法.方法:血样经C18-SPE小柱萃取后,在Beckman ODS色谱柱上,以甲醇-水(60∶40)为流动相,流速1.0 ml*min-1,231 nm检测.结果:氯硝西泮、地西泮的保留时间分别为4.5,7.4 min;线性范围分别为0.03~1.00,0.25~10.00 μg*ml-1;平均日内RSD分别为2.40%,2.30%;平均日间RSD分别为2.62%,2.49%;平均回收率分别为99.7%,99.5%,平均RSD分别为3.28%,3.36%.结论:本方法可用于同时监测地西泮与氯硝西泮血药浓度.

  1. Cytotoxic Effect of Erythroxylum suberosum Combined with Radiotherapy in Head and Neck Cancer Cell Lines. (United States)

    Macedo, Taysa B C; Elias, Silvia T; Torres, Hianne M; Yamamoto-Silva, Fernanda Paula; Silveira, Dâmaris; Magalhães, Pérola O; Lofrano-Porto, Adriana; Guerra, Eliete N S; Silva, Maria Alves G


    The mouth and oropharynx cancer is the 6th most common type of cancer in the world. The treatment may involve surgery, chemotherapy and radiotherapy. More than 50% of drugs against cancer were isolated from natural sources, such as Catharanthus roseus and epipodophyllotoxin, isolated from Podophyllum. The biggest challenge is to maximize the control of the disease, while minimizing morbidity and toxicity to the surrounding normal tissues. The Erythroxylum suberosum is a common plant in the Brazilian Cerrado biome and is popularly known as "cabelo-de-negro". The objective of this study was to evaluate the cytotoxic activity of Erythroxylum suberosum plant extracts of the Brazilian Cerrado biome associated with radiotherapy in human cell lines of oral and hypopharynx carcinomas. Cells were treated with aqueous, ethanolic and hexanic extracts of Erythroxylum suberosum and irradiated at 4 Gy, 6 Gy and 8 Gy. Cytotoxicity was evaluated by MTT assay and the absorbance was measured at 570 nm in a Beckman Counter reader. Cisplatin, standard chemotherapy, was used as positive control. The use of Erythroxylum suberosum extracts showed a possible radiosensitizing effect in vitro for head and neck cancer. The cytotoxicity effect in the cell lines was not selective and it is very similar to the effect of standard chemotherapy. The aqueous extract of Erythroxylum suberosum, combined with radiotherapy was the most cytotoxic extract to oral and hypopharynx carcinomas. PMID:27007356

  2. 对甲氧基苯磺酰氯柱前衍生生物胺的高效液相色谱分离%Separation of biogenic amines by high performance liquid chromatography with 4-methoxy benzenesulphonyl chloride as a precolumn derivatization reagent

    Institute of Scientific and Technical Information of China (English)

    屠春燕; 袁艳娟; 吴燕娇; 唐美华; 韦萍; 李寿椿


    采用对甲氧基苯磺酰氯作为柱前衍生试剂,RP-HPLC为分析模式,建立了一种新的生物胺衍生化方法,并对葡萄酒中生物胺含量进行检测. 通过液质联用对产物进行定性,研究并确定了最适衍生化条件:衍生温度50 ℃,缓冲液pH 9.0,衍生时间15 min. 实验建立了7种生物胺的HPLC分离方法:BeckmanODS柱;流动相A为10 mmol/L的NH_4Ac溶液(pH 6.37),B相为乙腈;采用梯度洗脱;流速1 mL/min;检测波长240 nm, 室温.

  3. Goeckerman's therapy for psoriasis with special reference to serum pentraxin 3 level

    Energy Technology Data Exchange (ETDEWEB)

    Ctirad, A.; Lenka, B.; David, P.; Zdenek, F.; Kveta, H.; Karel, E.; Jan, K. [Charles University Prague, Hradec Kralove (Czech Republic). University Hospital


    Goeckerman's therapy (GT) of psoriasis is based on daily application of pharmacy grade coal tar on affected skin with subsequent exposure to UV light. Pentraxin 3 (PTX3) is a newly identified acute phase reactant with non redundant functions in innate immunity. PTX3 has been shown to be a reliable prognostic marker in patients with various inflammatory disorders including rheumatoid arthritis, vasculitis, and psoriasis. The aim of this study was to evaluate the influence of Goeckerman's therapy of psoriasis on levels of two pentraxins: long pentraxin PTX3 and C reactive protein in 49 patients with chronic plaque psoriasis. CRP was assessed by immunonephelometry on IMMAGE 800 (Beckman, USA). PTX3 was detected using sandwich ELISA detection set (Alexis Biochemicals, Switzerland). The serum levels of both parameters (expressed as average {+-} 1 SD) were significantly diminished after GT. The level of PTX3 dropped from 1.92 {+-} 0.72 ng/ml before GT to 1.66 {+-} 0.58 ng/ml after GT (P = 0.0396) and the level of CRP fell from 4.64 {+-} 3.93 mg/l to 1.66 {+-} 0.58 mg/l (P {lt} 0.0001). Comparing to healthy controls, the serum levels of both parameters before GT were significantly higher than those found in healthy blood donors and remained significantly increased after GT. Increased serum concentrations of pentraxin 3 and CRP are alleviated by GT in patients with psoriasis.

  4. Low ionizing radiation influence on the nucleic acids in common Acacia

    International Nuclear Information System (INIS)

    Three months old common acacia saplings were selected from a forestry nursery with an uniform genetic background. A 60 Co ionizing radiation source, with a dose rate of 10 mCi was used to irradiate saplings for different time durations: 1h, 2h, 4h and 7 h. One day after the irradiation, small amounts of green tissue were took for nucleic acid extraction and assay. The quantitative extraction was performed in perchloric acid at a temperature of about 100 Celsius degrees. Centrifugation at 5,000 cycles/minute was performed and supernatant liquid was used for spectrophotometric assay. The light extinction at the wavelengths of 270 nm and 290 nm was measured, after Spirin's method using a Beckman spectrophotometer. The average between DNA and RNA content values was evaluated and represented graphically. The logarithmic representation of nucleic acids content, via exposure time, fitted with a mathematical polynomial function of second order, showed the decreasing of nucleic acids content in samples in comparison to the control. This could be the effect of radiation damage at the level of the nucleic acids primary structure, resulting in the reducing of nucleic acid amount in the vegetal cells of irradiated common acacia saplings. Direct radiation action on some chemical bonds as well as indirect effects mediated by water radiolysis could be implied in the diminution of DNA and RNA content from common acacia cell nuclei. (authors)

  5. Amino acid contents and transport of fixed N in nodules of Leucaena leucocephala variety K-8

    International Nuclear Information System (INIS)

    Seedlings of Leucaena leucocephala var. K-8 were grown with a N-free fertilizer or fertilizer containing 15N-depleted (NH4)2SO4 (0.01 atom 15N; 10 ppm). The nodules of 5 month old trees grown on N-free media were used for 15N-enriched treatment and as controls. Nodules from plants grown on 15N-depleted media were also used. Nodules were extracted with 0.5% aqueous toluene and aliquots were analyzed with a Beckman 120B Amino Acid Analyzer. Samples were separated into free ammonium, Asp-N, Glu-N, Asn and Gln amide- and amino-N, and remaining amino acids. Fractions were then analyzed for 15N content. Asn (27.3 umol/gfw) represented 56% of the total free amino acid pool in the nodules. Asn (amide-N and amino-N) also represented approximately 77% of the total N fixed during the one hour 15N-enriched N2 and the 15N-depleted treatments. Based on these findings and the fact that the ureide fraction is barely detectable in the nodules (0.25 μmol/gfw), the authors considers L. leucocephala an amide transporter of fixed N. Additional information will be presented on the amino acid contents of tissues, as well as a time course of amino acid content from seed through nodulation

  6. Evaluation of Thermal Control Coatings and Polymeric Materials Exposed to Ground Simulated Atomic Oxygen and Vacuum Ultraviolet Radiation (United States)

    Kamenetzky, R. R.; Vaughn, J. A.; Finckenor, M. M.; Linton, R. C.


    Numerous thermal control and polymeric samples with potential International Space Station applications were evaluated for atomic oxygen and vacuum ultraviolet radiation effects in the Princeton Plasma Physics Laboratory 5 eV Neutral Atomic Oxygen Facility and in the MSFC Atomic Oxygen Drift Tube System. Included in this study were samples of various anodized aluminum samples, ceramic paints, polymeric materials, and beta cloth, a Teflon-impregnated fiberglass cloth. Aluminum anodizations tested were black duranodic, chromic acid anodize, and sulfuric acid anodize. Paint samples consisted of an inorganic glassy black paint and Z-93 white paint made with the original PS7 binder and the new K2130 binder. Polymeric samples evaluated included bulk Halar, bulk PEEK, and silverized FEP Teflon. Aluminized and nonaluminized Chemfab 250 beta cloth were also exposed. Samples were evaluated for changes in mass, thickness, solar absorptance, and infrared emittance. In addition to material effects, an investigation was made comparing diffuse reflectance/solar absorptance measurements made using a Beckman DK2 spectroreflectometer and like measurements made using an AZ Technology-developed laboratory portable spectroreflectometer.

  7. Assay-specific artificial neural networks for five different PSA assays and populations with PSA 2-10 ng/ml in 4,480 men. (United States)

    Stephan, Carsten; Xu, Chuanliang; Cammann, Henning; Graefen, Markus; Haese, Alexander; Huland, Hartwig; Semjonow, Axel; Diamandis, Eleftherios P; Remzi, Mesut; Djavan, Bob; Wildhagen, Mark F; Blijenberg, Bert G; Finne, Patrik; Stenman, Ulf-Hakan; Jung, Klaus; Meyer, Hellmuth-Alexander


    Use of percent free PSA (%fPSA) and artificial neural networks (ANNs) can eliminate unnecessary prostate biopsies. In a total of 4,480 patients from five centers with PSA concentrations in the range of 2-10 ng/ml an IMMULITE PSA-based ANN (iANN) was compared with other PSA assay-adapted ANNs (nANNs) to investigate the impact of different PSA assays. ANN data were generated with PSA, fPSA (assays from Abbott, Beckman, DPC, Roche or Wallac), age, prostate volume, and DRE status. In 15 different ROC analyses, the area under the curve (AUC) in the PSA ranges 2-4, 2-10, and 4-10 ng/ml for the nANN was always significantly larger than the AUC for %fPSA or PSA. The nANN and logistic regression models mostly also performed better than the iANN. Therefore, for each patient population, PSA assay-specific ANNs should be used to optimize the ANN outcome in order to reduce the number of unnecessary biopsies. PMID:17333205

  8. Color Parameters of the Chromascop Shade Guide

    Directory of Open Access Journals (Sweden)

    William J. O'Brien


    Full Text Available The purposes of this study are: (1 determine the color of the twenty shades in the Ivoclar’s Chromascop shade guide, (2 determine the color representation of the shade guide described as coverage error (CE, and (3 compare this shade guide with the Vita Classical and Bioform shade guides. The spectral data was collected using Beckman model DU reflectance spectrophotometer equipped with an integrating sphere. Commission International de l’Eclairage (CIE chromaticity coordinates were calculated using CIE illuminant C and 1931 observer data, then converted to CIE L*a*b* and Munsell notation. Each shade was spectrophotometrically compared to the published colors of 335 human teeth. The minimum CIE L*a*b* color difference was calculated for each tooth and the average of these color differences was defined as the CE. The measured colors of the Chromascop guide had a CIE L* range of 79.67 to 65.61, an a* range of -0.71 to 3.85, and a b* range of 14.58 to 27.69. The average CE of the Chromascop shade guide was 3.38. The Chromascop shade guide has similar colors and a CE compared with the Bioform and Vita Classical shade guides, but with some shades of higher red and yellow components.

  9. Roger Hayward and the Invention of the Two-Mirror Schmidt (United States)

    Bell, T. E.


    Roger Hayward (1899-1979), now virtually unknown, was a multitalented architect, scientific illustrator, and optical inventor. Remembered primarily for illustrating Scientific American magazine's Amateur Scientist column between 1949 and 1974, he also illustrated more than a dozen textbooks in optics, physics, geology, oceanography, and chemistry, several of which became classics in their fields. He designed façades with astronomical themes for major buildings in Los Angeles, California, and sculpted mammoth, realistic models of the moon for Griffith Observatory, Adler Planetarium, and Disneyland. Throughout his life, he recreationally painted watercolors and oils that at least one critic likened to the work of John Singer Sargent. Hayward is least known as an optical designer, yet he made significant contributions to the DU spectrophotometer that established the multimillion-dollar company Beckman Instruments. During the pre-radar days of World War II at Mount Wilson Observatory, Hayward invented a classified Cassegrain version of the Schmidt telescope especially adapted for nighttime infrared aerial photography, plus extraordinarily simple machines that allowed inexperienced soldiers to grind, polish, and test accurate aspheric Schmidt correcting plates at speeds compatible with mass production - and later received U.S. patents for them all. This paper, drawn in part from unpublished letters between Hayward and Albert G. Ingalls, will feature little-known images of Hayward's work.

  10. Graphene Quantum Point Contact Transistor for DNA Sensing (United States)

    Girdhar, Anuj


    Over the past few years the need has grown for low-cost, high-speed, and accurate biomolecule sensing technology. Graphene is a promising choice for use in such sensing applications, as its single-atom thickness and unique electronic structure is suitable for probing biomolecules like DNA at a very high resolution. We propose the design of a transistor containing a graphene nanoribbon sensing layer with a nanopore for the simultaneous detection and control of a translocating DNA molecule. Through the combination of molecular dynamics simulations, a self-consistent Poisson equation solver, and electronic transport theory, we show that the motion of a DNA molecule through a nanopore can be observed by measuring conductance modulations in the graphene nanoribbon. We also demonstrate that the sensitivity of the graphene sheet conductance to external charges can be enhanced by modulating its carrier concentration as well as by choosing a quantum point contact geometry for the graphene nanoribbon. In addition, we propose the use of extra gates to control both the lateral and translocating motion of a DNA molecule inside the nanopore. I would like to acknowledge Oxford Nanopore Technology as well as the Beckman Institute for Advanced Science for their support.

  11. Synthesis of immunomagnetic nanoparticles and their application in the separation and purification of CD34 + hematopoietic stem cells (United States)

    Chen, Wei; Shen, Hebai; Li, Xingyu; Jia, Nengqin; Xu, Jianming


    The silica-coated superparamagnetic nanoparticles with the uniform diameter of about 60 nm were synthesized by reverse microemulsions method. And the magnetic nanoparticles were modified with N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (AEAPS). The immunomagnetic nanoparticles were then successfully prepared by covalently immobilizing anti-CD34 + monoclonal antibodies to the surface of amino silane modified magnetic particles. The cell separation results showed that the synthesized immunomagnetic nanoparticles could rapidly and conveniently separate the CD34 + cells with high efficiency and specificity than normal ones. The surface morphology of separated target cells was examined by scanning electron microscope (SEM). Atomic force microscope (AFM) also characterized the magnetic materials on the surface of the separated target cells for the first time, which further confirmed that the target cells were separated by the immunomagnetic nanoparticles. The viability of the separated cells was studied by culturing method and Beckman Vi-cell viability analyst. Therefore, our experiments provided a new, direct, rapid mode to separate target cells.

  12. The effect of seed crystals of hydroxyapatite and brushite on the crystallization of calcium oxalate in undiluted human urine in vitro: implications for urinary stone pathogenesis. (United States)

    Grover, Phulwinder K.; Kim, Dong-Sun; Ryall, Rosemary Lyons


    BACKGROUND: The aim of this study was to determine whether crystals of hydroxyapatite (HA) or brushite (BR) formed in urine promote the epitaxial deposition of calcium oxalate (CaOx) from undiluted human urine in vitro and thereby explain the occurrence of phosphate in the core of urinary stones consisting predominantly of CaOx. MATERIALS AND METHODS: Crystals of HA, BR, and CaOx were generated from human urine and their identity confirmed by X-ray analysis. Standard quantities of each crystal were then added to separate aliquots of pooled undiluted human urine and CaOx crystallization was induced by the addition of identical loads of sodium oxalate. Crystallization was monitored by Coulter Counter and (14) C-oxalate analysis and the precipitated crystals were examined by scanning electron microscopy. RESULTS: In comparison with the control to which no seeds were added, addition of CaOx crystals increased the deposition of (14) C-oxalate by 23%. On the other hand, seeds of HA and BR had no effect. These findings were supported by Coulter Counter analysis, which showed that the average modal sizes of crystal particles precipitated in the presence of HA and BR seeds were indistinguishable from those in the control, whereas those deposited in the presence of CaOx were significantly larger. Scanning electron microscopy confirmed these results, demonstrating that large aggregates of CaOx dihydrates were formed in the presence of CaOx seeds, whereas BR and to a lesser extent HA seeds were scattered free on the filtration membrane and attached like barnacles on the surface of the freshly precipitated CaOx crystals. CONCLUSION: Seed crystals of HA or BR do not promote CaOx deposition in urine in vitro and are therefore unlikely to influence CaOx crystal formation under physiologic conditions. However, binding of HA and BR crystals to, and their subsequent enclosure within, actively growing CaOx crystals might occur in vivo, thereby explaining the occurrence of mixed

  13. Encapsulation of Platelet in Kefiran Polymer and Detection of Bioavailability of Immobilized Platelet in Probiotic Kefiran as A New Drug for Surface Bleeding

    Directory of Open Access Journals (Sweden)

    Anahita Jenab


    Full Text Available Background : Kefir contains lactic acid bacteria (Lactobacillus, Lactococcus, Leuconostoc, Acetobacter and Streptococcus and yeasts (Kluyveromyces, Torula, Candida, Saccharomyces .Kefiran is the polysaccharide produced by lactic acid bacteria in kefir.Methods : Kefiran was prepared from milk containing 0.5% fat and 10 grams kefir grains and was separated from kefir by ethanol (0.02 gram following entrapping the platelets to this polymer. Ligand of the platelet-polysaccharide was studied by FTIR.Results : FTIR results showed that the bands of C-O and C-O-C connections were formed and the polysaccharides had been attached to the receptors of the platelet glycoproteins (GP Ib,GPIIb / IIIa. Stability and encapsulation of the platelet and kefiran were assessed by Coulter Counter. Encapsulation of the platelets by polysaccharide at the beginning caused to reduce the number of platelets following by releasing of 50% of the platelets after 3 hours.Conclusion : The platelets were encapsulated in kefiran polymer and detected for bioavailability as new drug for surface bleeding. Also, kefiran has antimicrobial and antifungal properties. On the other hand, the existence of nisin in kefiran could be useful as an antibacterial lantibiotic. 

  14. Standard methods for chemical, mass spectrometric, and spectrochemical analysis of, and physical tests on, beryllium oxide powder

    International Nuclear Information System (INIS)

    Methods are described for the determination of C, Fe, N, Cl-, Li, S, thirty trace elements, and the amount of BeO in BeO powder. Methods are included for certain physical properties of the oxide powders. C is determined by the combustion-thermal conductivity method. A colorimetric method using orthophenanthroline is used for the determination of Fe, and N is determined by a micro Kjeldahl method. Chlorine is determined nephlometrically using AgNO3, and Li is determined by atomic absorption spectrophotometry. A complete burning spectrochemical method and a spark-source mass spectrographic method were used to analyze for the thirty trace elements. The density of the BeO powders was measured by a toluene displacement method, and a tap-pak volumetric method was used for the determination of the pour and tap densities. Two different methods described for the particle-size composition determination are Coulter Counter method and sieve analysis method. Methods for determination of bulk density, real density, porosity, and pore size of the solid oxides are described. A nitrogen absorption method is described for the determination of surface area

  15. How does the greater white-toothed shrew, Crocidura russula, responds to long-term heavy metal contamination? -- A case study. (United States)

    Marques, Carla Cristina; Sánchez-Chardi, Alejandro; Gabriel, Sofia Isabel; Nadal, Jacint; Viegas-Crespo, Ana Maria; da Luz Mathias, Maria


    Heavy metals accumulation in parallel with the evaluation of physiological and biochemical effects resulting from continued metal exposure were considered here using for the first time the great white-toothed shrew Crocidura russula as an in vivo model. Shrews were originated from an abandoned lead/zinc mining area and from a reference area, both in Alentejo, southern Portugal. Hepatic contents of nickel, copper, zinc, cadmium, mercury and lead were quantified by Inductively Coupled Plasma Mass Spectrometer (ICP-MS). Haematological parameters (white blood cells, red blood cells, haemoglobin and haematocrit) were obtained in a Coulter Counter Analyser and biochemical markers of the redox balance (glutathione S-transferase, glutathione peroxidase, and glutathione reductase) activities were measured spectrophotometrically using a Duo-50 spectrophotometer. Compared with control animals, significantly higher concentration of hepatic cadmium (9.29 vs. 1.18 micorg/g dry weight) and nickel (1.56 vs. 0.343 microg/g dry weight) were detected in the shrews collected in the mining area. However, no significant changes were observed on haematological or enzymatic parameters in animals exposed to metal pollution. The obtained results show that shrews are good bioaccumulators of toxic heavy metals, but very tolerant to their effects, revealing an interesting long-term adaptation to polluted environments. In addition, this study provides reference values for haematological parameters and antioxidant enzymes levels in C. russula, which may be relevant for comparative purposes in further studies. PMID:17321569

  16. Automated platelet counters: a comparative evaluation of latest instrumentation. (United States)

    Mayer, K; Chin, B; Magnes, J; Thaler, H T; Lotspeich, C; Baisley, A


    An extensive evaluation of performance characteristics and accuracy of clinical results for two automated multiparameter whole-blood cell counters (the Coulter Counter Model S-Plus and the Ortho ELT-8) and two single-parameter semiautomated platelet counters (the J. T. Baker MK-4/HC and the Clay-Adams Ultra-Flo 100) is described. Results of comparative assays performed on more than 1,200 clinical specimens are analyzed. These results are compared with manual determinations where appropriate. Particular attention is accorded to the accuracy of platelet counts, especially at abnormal levels below 70 X 10(3)/cu mm, where falsely elevated platelet counts may lead to serious clinical consequences. Both multiparameter instruments yielded accurate results, with the exception of low values reported by the ELT-8 for mean corpuscular volumes above 100 cu micrometer. Results for platelet counts were accurate for most specimens on all four instruments; the ELT-8 was the most reliable (P < 0.01), especially for the critically low counts. Although no instrument is infallible in determining platelet counts at all levels, the authors conclude that addition of platelet-counting capability represents a significant advancement over existing instrumentation. PMID:7405891

  17. A side-by-side evaluation of four platelet-counting instruments. (United States)

    Dalton, W T; Bollinger, P; Drewinko, B


    The performances of four instruments for counting platelets were evaluated in a side-by-side study: the Haema-Count MK-4/HC, an electronic impedance instrument that counts platelets in platelet-rich plasma; the Ultra-Flo 100, and the Coulter Counter Model S-Plus, electronic impedance instruments that count platelets in the presence of intact erythrocytes; and the AutoCounter, an optical instrument that counts platelets in the presence of lysed erythrocytes. The Ultra-Flo 100 and the S-Plus showed the best within-run precision, and all four instruments were considerably more precise than manual platelet counting, especially at low levels of platelet count. The four instruments were all linear in the ranges tested (5 to 650 x 10(9)/or greater), and sample carry-over was less than 0.7% for each. A noteworthy finding was that the erythrocyte concentration of the blood samples affected the displayed platelet count of the S-Plus and, to a lesser extent, that of the AutoCounter, in a predictable way, whereas it did not greatly affect the displayed count of the Ultra-Flo 100. In addition to differences in quality of performances, the four instruments differed considerably in speed and ease of operation and in cost. PMID:7405890


    Directory of Open Access Journals (Sweden)

    Gorea M.


    Full Text Available Forsterite (Mg2SiO4 powder has been synthesized from talc (Mg3Si4O10(OH2 and magnesium carbonate (MgCO3 by applying solid state reactions method. The raw materials mixture has been milled for 10 hours until nano powders have been obtained. This mixture was then thermally treated at various temperatures. The synthesized material was structurally characterized by X-ray diffraction. Forsterite represented the main crystalline phase in the samples fired to 1100 and 1200°C, while at 1000°C small amounts of enstatite and periclase were still identified. The particles size and morphology were investigated by TEM, SEM and AFM, and the grain size distribution with a Counter Coulter-type laser granulometer. A ratio of 75 % of the particles in the samples fired to 1000, and respectively 1100°C were less than 25 nm, while the maximum size was 42 nm. For the samples fired at 1200°C, most of the particles (74 % were larger, about 6.8 μm, with a maximum of 70 μm. In order to evaluate its bioactivity, we have immersed the forsterite powder into simulating body fluids (SBF. Following 28 days of experiment, the FTIR spectra collected on the forsterite nanopowder contained the specific hydroxylapatite bands, while in the case of the micro powder these bands are hardly visible.

  19. High-level 13C-enrichment of random and synchronous populations of Chlamydomonas reinhardii

    International Nuclear Information System (INIS)

    The alga Chlamydomonas reinhardii was grown in suspension culture at high levels of 13C-enrichment (98 mol percent) both in synchronous and random populations for the purpose of investigating possible macro- and ultrastructural changes in the cell as induced by essentially total carbon replacement. The algae, grown in spinner flasks, were analyzed using a newly developed multiparameter flow-system technique applied to characterizing various algal genera. The versatility of this technique provides for measuring and processing several cell characteristics simultaneously and separating cells according to selected combinations of parameters. In these studies, cell volume (by Coulter aperture) and DNA and chlorophyll content were determined simultaneously. Cell ultrastructure was examined at various levels of isotope enrichment and time periods by electron microscopy. The data presented for synchronous growth of this organism demonstrate the absence of biological effects (considering the parameters measured) due to the almost total replacement of cellular 12C with 13C. Interpretational problems encountered when looking for biological effects on random populations are discussed

  20. Dissociations among linguistic, cognitive, and auditory-motor neuroanatomical domains in children who stutter. (United States)

    Choo, Ai Leen; Burnham, Evamarie; Hicks, Kristin; Chang, Soo-Eun


    The onset of developmental stuttering typically occurs between 2 to 4 years of age, coinciding with a period of rapid development in speech, language, motor and cognitive domains. Previous studies have reported generally poorer performance and uneven, or "dissociated" development across speech and language domains in children who stutter (CWS) relative to children who do not stutter (CWNS) (Anderson, Pellowski, & Conture, 2005). The aim of this study was to replicate and expand previous findings by examining whether CWS exhibit dissociated development across speech-language, cognitive, and motor domains that are also reflected in measures of neuroanatomical development. Participants were 66CWS (23 females) and 53CWNS (26 females) ranging from 3 to 10 years. Standardized speech, language, cognitive, and motor skills measures, and fractional anisotropy (FA) values derived from diffusion tensor imaging from speech relevant "dorsal auditory" left perisylvian areas (Hickok & Poeppel, 2007) were analyzed using a correlation-based statistical procedure (Coulter, Anderson, & Conture, 2009) that quantified dissociations across domains. Overall, CWS scored consistently lower on speech, language, cognitive and motor measures, and exhibited dissociated development involving these same measures and white matter neuroanatomical indices relative to CWNS. Boys who stutter exhibited a greater number of dissociations compared to girls who stutter. Results suggest a subgroup of CWS may have incongruent development across multiple domains, and the resolution of this imbalance may be a factor in recovery from stuttering. PMID:27010940

  1. Between two worlds: Yamanouchi Shigeo and eugenics in early twentieth-century Japan. (United States)

    Otsubo, Sumiko


    This paper explores the eugenic through of Yamanouchi Shigeo (1876-1973), who was trained in plant cytology under the tutelage of botanist and eugenicist John Coulter (1851-1928) in the USA, and later become one of the early and important popularizers of eugenic ideas in Japan. His career demonstrates a direct link between Japanese and US eugenics. Despite his academic training and research at various internationally renowned institutions, numerous publications, and longevity, his life has received little scholarly attention. By the early twentieth century, most biologists in Japan, as in the USA, began accepting Mendelian evolutionary theory and rejecting the Lamarckian notion of inheritance of acquire characteristics. However, Yamanouchi Shigeo's eugenic view represents a paradox: he was a mendelian cytologist sympathetic to Lamarckism. Was his 'nurture'-oriented eugenic view unscientific? is that why he was largely ignored in the history of botany in Japan? This study attempts to answer these questions and to analyse the origins and distinct features of Yamanouchi's eugenic ideas by situating Yamanouchi's eugenic through historically and culturally. After examining his scientific papers, popular writings, and documents of various organizations to which he belonged, I argue that Yamanouchi's 'softer' (or less biologically deterministic) perspective may have reflected the Japanese desire to catch up with the dominant 'race' by using eugenics without accepting permanent inferior status. PMID:15789487


    Directory of Open Access Journals (Sweden)

    Gianvico Melzi d’Eril


    Full Text Available Background. Sport’s anemia is a common risk for athletes. The principal source of an accelerated turnover of the erythrocytes in sportsmen is the intravascular hemolysis. This phenomenon is induced by mechanical breakage for impact of feet and muscular contractions, but also by osmotic changes causing membrane fragility, typically evident after exercise, when free radicals are increased. Whole-body cryotherapy (WBC covers a wide range of therapeutic applications and consists of briefly exposing the body to extremely cold air. In sports medicine, WBC is used to improve recovery from muscle injury; however, empirical studies on its application to this area are lacking. Design and Methods. We recruited ten rugby players of the Italian National Team. In these athletes we measured hematological parameters, before including mean sphered cell volume (MSCV by means of Coulter LH750, besides of haptoglobin, and after WBC. The subjects underwent five sessions on alternate days once daily for one week. During the study period, the training workload was the same as that of the previous weeks. Results. We observed in the athletes increase of haptoglobin and an increase of MSCV after the treament period. Conclusions. WBC reduces sports haemolysis, as judged from MSCV and haptoglobin data, supported from other haematological values, as well as the absence of mean corpuscolar volume and reticulocytes increase. The treatment is useful to prevent the physiological impairments derived from sport haemolysis.

  3. Changes of Hematological Markers during a Multi-stage Ultra-marathon Competition in the Heat. (United States)

    Rama, L M; Minuzzi, L G; Carvalho, H M; Costa, R J S; Teixeira, A M


    This study examined the changes in resting hematological variables in ultra-endurance runners throughout a multi-stage ultra-marathon competition, and compared athletes that completed all stages with those that failed to complete at least one stage within the cut-off time of competition. 19 ultra-endurance runners competing in a 230 km multi-stage ultra-marathon, conducted over 5 consecutive days in hot ambient conditions (32-40°C T(max)), volunteered to participate in the study. Each day, whole blood samples were collected prior to stage commencement and analyzed for full cell counts by Coulter counter. Linear increases were observed for leukocytes, monocytes and lymphocytes; with increases until Stage 3 and a decrease thereafter. Granulocytes showed a cubic growth exponent, indicating decrements to baseline after the significant increments until Stage 3. Hemoglobin and hematocrit showed linear decrements throughout the multi-stage ultra-marathon. No changes in erythrocytes and platelets were observed throughout the multi-stage ultra-marathon. Granulocytes, erythrocytes, hemoglobin and hematocrit changes along the multi-stage ultra-marathon differed in runners that completed all stages compared to those who failed to complete at least one stage within the cut-off time. Multi-stage ultra-marathon in the heat has a large impact on hematological responses of ultra-endurance runners associated with altered performance. PMID:26509375

  4. Removal of focal atheromatous lesions by angioscopically guided high-speed rotary atherectomy. Preliminary experimental observations

    International Nuclear Information System (INIS)

    A new high-speed rotary atherectomy device, inserted over a guide wire and directed with an angioscope, offers the potential of restoring patency of outflow vessels by boring out the atheromatous lesion of the orifices of runoff vessels. The device was tested on 68 cadaver arteries with atheromatous lesions involving the superficial femoral, popliteal, and tibial arteries. This was performed with either free segments or in situ with the device placed through a popliteal arteriotomy. The gross results of rotary atherectomy were assessed by angioscopy, angiography, or both. The luminal surfaces were studied with scanning electron microscopy and transverse sections of vessels were studied with light microscopy. The pulverized atheroma, in colloidal suspension, was analyzed for particle size by Coulter counter. The effect of a colloidal suspension of atheromatous particles on distal capillary circulation was measured in animal experiments. Obstructive lesions were successfully removed in 36 of 37 stenotic arteries (97%) and 18 of 31 completely occluded arteries (58%), an overall efficacy of 54 of 68 (79%). In successfully atherectomized arteries, angioscopy and angiography demonstrated a widely patent, smooth, polished surface. Light microscopy demonstrated removal of the diseased intima with maintenance of the outer media and adventitia. The pulverized atheroma particles were generally smaller than red blood cells and injection of the colloidal atheroma into canine femoral arteries failed to produce local tissue injury. We conclude that in the human cadaver this atherectomy device effectively enlarges and recanalizes obstructed superficial femoral, popliteal, and tibial arteries

  5. Microfluidic CODES: a scalable multiplexed electronic sensor for orthogonal detection of particles in microfluidic channels. (United States)

    Liu, Ruxiu; Wang, Ningquan; Kamili, Farhan; Sarioglu, A Fatih


    Numerous biophysical and biochemical assays rely on spatial manipulation of particles/cells as they are processed on lab-on-a-chip devices. Analysis of spatially distributed particles on these devices typically requires microscopy negating the cost and size advantages of microfluidic assays. In this paper, we introduce a scalable electronic sensor technology, called microfluidic CODES, that utilizes resistive pulse sensing to orthogonally detect particles in multiple microfluidic channels from a single electrical output. Combining the techniques from telecommunications and microfluidics, we route three coplanar electrodes on a glass substrate to create multiple Coulter counters producing distinct orthogonal digital codes when they detect particles. We specifically design a digital code set using the mathematical principles of Code Division Multiple Access (CDMA) telecommunication networks and can decode signals from different microfluidic channels with >90% accuracy through computation even if these signals overlap. As a proof of principle, we use this technology to detect human ovarian cancer cells in four different microfluidic channels fabricated using soft lithography. Microfluidic CODES offers a simple, all-electronic interface that is well suited to create integrated, low-cost lab-on-a-chip devices for cell- or particle-based assays in resource-limited settings. PMID:27021807

  6. Dust flux in peripheral East Antarctica: preliminary results from GV7 ice core and extension of the TALDICE dust record to the sub-micron range (United States)

    Delmonte, Barbara; Giovanni, Baccolo; Fausto, Marasci; Iizuka, Yoshinori; Valter, Maggi


    Improved understanding of climate variability over the last two millennia - that is a critical time period for investigating natural and anthropogenic climate change - is one of the key priorities of the International Partnership in Ice Core Sciences (IPICS). The Italian contribution to this concerted international effort is represented by the project IPICS-2kyr-Italy supported by PNRA. In this context, a novel intermediate core (about 250 m deep) was drilled during the 2013/14 field season at the peripheral site of GV7 in East Antarctica (70°41'S, 158°52'E; elevation 1950 m), where snow accumulation is very high (about 3 times Talos Dome, 10 times EPICA Dome C). After the ice core processing campaign at EuroCold (UNIMIB) carried out in synergy between Italy and Korea teams, measurements of dust concentration and size distribution are now in progress. A novel Coulter Counter apparatus has been set up in order to extend dust size spectra down to 600 nm. Samples are analyzed immediately after melting and also 24h later under identical conditions, for a quantitative assessment of the contribution of water-soluble microparticles (salts). Seasonal variability and trends of insoluble dust, metastable salts and size distribution of these compounds is under study. The possibility to extended the size range of dust measurements has allowed refining estimates of dust flux at Talos Dome and an adjustment of published data.

  7. Single Nanoparticle Translocation Through Chemically Modified Solid Nanopore. (United States)

    Tan, Shengwei; Wang, Lei; Liu, Hang; Wu, Hongwen; Liu, Quanjun


    The nanopore sensor as a high-throughput and low-cost technology can detect single nanoparticle in solution. In the present study, the silicon nitride nanopores were fabricated by focused Ga ion beam (FIB), and the surface was functionalized with 3-aminopropyltriethoxysilane to change its surface charge density. The positively charged nanopore surface attracted negatively charged nanoparticles when they were in the vicinity of the nanopore. And, nanoparticle translocation speed was slowed down to obtain a clear and deterministic signal. Compared with previous studied small nanoparticles, the electrophoretic translocation of negatively charged polystyrene (PS) nanoparticles (diameter ~100 nm) was investigated in solution using the Coulter counter principle in which the time-dependent nanopore current was recorded as the nanoparticles were driven across the nanopore. A linear dependence was found between current drop and biased voltage. An exponentially decaying function (t d   ~ e (-v/v0) ) was found between the duration time and biased voltage. The interaction between the amine-functionalized nanopore wall and PS microspheres was discussed while translating PS microspheres. We explored also translocations of PS microspheres through amine-functionalized solid-state nanopores by varying the solution pH (5.4, 7.0, and 10.0) with 0.02 M potassium chloride (KCl). Surface functionalization showed to provide a useful step to fine-tune the surface property, which can selectively transport molecules or particles. This approach is likely to be applied to gene sequencing. PMID:26831688

  8. Cytotoxic effect of the pentacyclic oxindole alkaloid mitraphylline isolated from Uncaria tomentosa bark on human Ewing's sarcoma and breast cancer cell lines. (United States)

    García Giménez, Dolores; García Prado, Elena; Sáenz Rodríguez, Teresa; Fernández Arche, Angeles; De la Puerta, Rocío


    Preparations from Uncaria tomentosa, a South American Rubiaceae, have been used in the Peruvian traditional medicine for the treatment of infective, inflammatory and tumoral processes. In this study, the pentacyclic oxindole alkaloid mitraphylline was isolated from the dried inner bark of this plant species, and its structure elucidated by analysis of NMR spectroscopic data. Mitraphylline was differentially identified from its stereoisomeric pair isomitraphylline by (15)N-NMR. Its antiproliferative and cytotoxic effects have been tested on human Ewing's sarcoma MHH-ES-1 and breast cancer MT-3 cell lines, using cyclophosphamide and vincristine as reference controls. A Coulter counter was used to determine viable cell numbers, followed by the application of the tetrazolium compound MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy phenyl)-2-(4-sulfophenyl)-2H-tetrazolium] an inner salt. A colorimetric method was employed to evaluate cell viability in this cytotoxic assay. Micromolar concentrations of mitraphylline (5 microM to 40 microM) inhibited the growth of both cell lines in a dose-dependent manner. The IC (50) +/- SE values were 17.15 +/- 0.82 microM for MHH-ES-1 and 11.80 +/- 1.03 microM for MT-3 for 30 hours, smaller than those obtained for the reference compounds. This action suggests that the pentacyclic oxindole alkaloid mitraphylline might be a new promising agent in the treatment of both human sarcoma and breast cancer. PMID:19724995

  9. Measurement and correlation of conditions for entrapment and mobilization of residual oil. Final report, January 1981-March 1984

    Energy Technology Data Exchange (ETDEWEB)

    Morrow, N.R.


    This is the final report of a two-year project which had four major task areas. A substantial portion of work carried out under this project has been reported in detail in journals, the First Annual Report to the Department of Energy or in manuscripts which have been submitted for publication and are available on request. In such cases only major conclusions are reported, along with reference to the detailed accounts. Work is reported for the following 4 major tasks: (1) residual saturation measured by laboratory core flooding (core flooding experiments, contact angle measurements); (2) effect of high pressure gradients on residual oil saturations (capillary number relationships, residual oil flushing at wellbore, electrical resistivities at reduced residual oil saturations); (3) mechanisms of mobilization and entrapment of residual oil (magnitude and detailed structure of residual oil saturations, effect of interfacial tension on the stability of displacement fronts, effect of pore shape on displacement curvatures); and (4) residual oil structure (analysis of blob-size distributions by Coulter counter, changes in residual oil structure with oil recovery). 53 references, 76 figures, 46 tables.

  10. Combined production og biomass for energy and clean drinking water - A miscanthus demonstration project on the 'Renewable energ island' Samsoe

    Energy Technology Data Exchange (ETDEWEB)

    Joergensen, U. [Danish Inst. of Agricultural sciences (Denmark)


    The island of Samsoe has been elected as the Danish 'Renewable Energy Island', with the aim of 100% self sufficiency with renewable energy within year 2007. The island is supposed to become a showcase for Danish renewable energy technology. The biomass resource of the island is however scarce due to intensive horticultural production and a rather small forested area, and there is a need to produce more biomass for the heating plants established or under construction. In year 2001 the Danish Energy Agency sponsored establishment of approximately 20 ha miscanthus on Samsoe, and the county of Aarhus sponsored the establishment of a soil water sampling station to follow nitrate leaching from two management systems of miscanthus and from a neighbouring crop rotation. This paper presents the project experiences and results so far. The project has produced the following experiences on miscanthus establishment: Rhizome harvest is best done on sandy soils; Calculation of amount of rhizome material collected and planted is difficult and more experience is needed; The planting machine is operational but there are several points for further optimization, e.g. stone release on coulters; So far the mild climate with low precipitation at Samsoe seems to suite miscanthus well. With respect to ground water protection the first results on nitrate concentrations are in line with earlier experimental results. A catch crop of fodder radish significantly reduced concentrations in the first year. (ba)

  11. Diffusion controlled deposition of particulate matter from flowing slurries

    International Nuclear Information System (INIS)

    Investigations have been carried out to determine the rate of deposition of micron sized magnetite particles from flowing slurry onto the inner walls of simulated heat exchanger tubes. Slurry was circulated in a closed-loop facility with deposition measurements being made in an aluminium tube of 19mm bore. Runs were conducted under isothermal conditions at temperatures between 293K and 350K over a Reynolds Number range 9,600 to 147,000. Slurry concentration was maintained in the range 200 to 600 mg/kg. The average magnetite particle size as measured on a Coulter Counter was approximately 1.5 μm. Deposit growth on the test section was measured by means of a low energy X-ray machine. The results showed high initial deposition rates accompanied by steadily increasing removal rates producing asymptotic curves. Analysis indicates that deposition is governed by the forced convective diffusion of particles to the tube wall. Correlation of deposition rates is possible using standard mass transfer equations. Deposits were friable and porous with void fractions ≅70%

  12. Scientific Analysis Cover Sheet for Radionuclide Screening

    International Nuclear Information System (INIS)

    pressurized water reactor (PWR) fuel, U.S. Department of Energy (DOE) spent nuclear fuel (DSNF), and high-level waste (HLW). Average and outlying (high burnup, high initial enrichment, low age, or otherwise exceptional) forms of each waste-form type are considered. This analysis has been prepared in accordance with a technical work plan (BSC 2002c). In a review of Revision 00 of this radionuclide screening analysis, the NRC found that ''processes that affect transport in the biosphere, such as uptake by plants and bioaccumulation are not accounted for'' and that ''the direct exposure pathway is not accounted for'' (Beckman 2001, Section The NRC also found that the solubility and sorption classes were too broadly defined, noting, for example, that Se is in the same solubility and sorptivity groups as Np and U, yet is ''more soluble than Np and U by several orders of magnitude'' (Beckman 2001, Section This revision seeks to build upon the strengths of the earlier screening method while responding to the specific concerns raised by the NRC and other reviewers. In place of simple inhalation and ingestion dose conversion factors, the revised radionuclide screening uses screening factors that also take into account soil accumulation, uptake by plants, exposure to contaminated ground, and other features of the biosphere that were neglected in the previous screening. Whereas the previous screening analysis allowed only two solubility classes (soluble and insoluble), the revised screening introduces an intermediate solubility class to better segregate the radionuclides into transport groups

  13. Influences of Different Instrument Standard Fluid hCG Molecular Variant On hCG Detection Results%不同的仪器标准液hCG分子变异体对hCG结果的影响

    Institute of Scientific and Technical Information of China (English)

    张忠源; 胡望平; 池细俤; 叶桂云; 胡盈莹; 闫炳龙; 张永平


    Objective To explore the rusults of human chorionicvilli gonadotropin (hCG) in serum (standard solution) with different detection system, different hospitals in Fujian Province and study their possible relationship with hCG relative molecular variants. Methods Beckman Access and Tosh AIA - 1800 immune machine detection system were used respectively to test the different concentrations of quality control things and the hCG of 44 patients. The hCG quality control data were collected from 2007 to 2009 in Fujian Province. And then literature to analysis the hCG molecular variants detection data were collected. Results Even with the same quality control things, the same serum and the same hCG relative molecular variants displayed different test results in different instrument detection system with a significant difference. The linear equation of Beckman ACCESS and Tosh AIA - 1800 was Y = 1. 3336 X - 70.199. The result of clinical serum hCG, quality control things in Tosh AIA - 1800 was 133. 8% , 137. 2% , and in Abbott was 132% . These two results were higher than those of the Beckmen ACCESS which displayed 100% . Conclusion The hCG molecular variants with different quality control things infulence the hCG detection results. Because of the hCG molecular variants, different instruments will display different hCG detection reuslts.%目的 探讨血清(标准物)中人绒毛膜促性腺激素(hCG) 在不同的检测系统、福建省各医院的测定结果,及其与hCG相关分子变异体可能的关系.方法 分别应用贝克曼Access、日本东曹(Tosh AIA-1800),在此两个免疫仪器检测系统检测不同浓度的质控物和44例患者血清的hCG;并收集福建省2007~2009年hCG质控数据、再收集文献hCG相关分子变异体检测数据进行分析.结果 同一质控物、同一血清、同一hCG相关分子变异体对不同的仪器检测系统,显示不同的测定结果,有显著差异.贝克曼Access与Tosh AIA-1800线性方程为Y=1

  14. A Retrospective Analysis of Urine Drugs of Abuse Immunoassay True Positive Rates at a National Reference Laboratory. (United States)

    Johnson-Davis, Kamisha L; Sadler, Aaron J; Genzen, Jonathan R


    Urine drug screens are commonly performed to identify drug use or monitor adherence to drug therapy. The purpose of this retrospective study was to evaluate the true positive and false positive rates of one of our in-house urine drug screen panels. The urine drugs of abuse panel studied consists of screening by immunoassay then positive immunoassay results were confirmed by mass spectrometry. Reagents from Syva and Microgenics were used for the immunoassay screen. The screen was performed on a Beckman AU5810 random access automated clinical analyzer. The percent of true positives for each immunoassay was determined. Agreement with previously validated GC-MS or LC-MS-MS confirmatory methods was also evaluated. There were 8,825 de-identified screening results for each of the drugs in the panel, except for alcohol (N = 2,296). The percent of samples that screened positive were: 10.0% for amphetamine/methamphetamine/3,4-methylenedioxy-methamphetamine (MDMA), 12.8% for benzodiazepines, 43.7% for opiates (including oxycodone) and 20.3% for tetrahydrocannabinol (THC). The false positive rate for amphetamine/methamphetamine was ∼14%, ∼34% for opiates (excluding oxycodone), 25% for propoxyphene and 100% for phencyclidine and MDMA immunoassays. Based on the results from this retrospective study, the true positive rate for THC drug use among adults were similar to the rate of illicit drug use in young adults from the 2013 National Survey; however, our positivity rate for cocaine was higher than the National Survey. PMID:26668238

  15. Optimización de la extracción de ADN de Passiflora ligularis para el análisis por medio de marcadores moleculares

    Directory of Open Access Journals (Sweden)

    Gina Solano-Flórez


    Full Text Available Extraction of high quality DNA of Passiflora ligularis for its analysis with molecular markers. Objective. To standardize a precise andefficient DNA isolation protocol of Passiflora ligularis. Materials and methods. Two methods of DNA extraction and two different tissuesof Passiflora ligularis were evaluated in terms of purity, quality and quantity of DNA yield, as well as DNA’s suitability for moleculartechniques based on PCR such as RAPDs. Quantification of DNA was done using absorbance spectrophotometry at a wavelength of260nm (A260 with a Beckman Du ® 530 spectrophotometer. An estimate of the DNA’s purity was obtained using the absorbance ratio(A260 / A280 nm. The variables analyzed in this study were the extraction method (A and the tissue type (B, in order to define their influenceon the purity and quantity of the DNA extracted. For the study of these variables a random design with a 2 x 2 factorial arrangement wasused. Results. The average quantities of DNA obtained with the modified method of Mc Couch et al. (1988 and the modified method ofDoyle & Doyle (1991 method were 778.9 μg/ml and 660.1 μg/ml respectively for dry tissue. Averages with fresh tissue were 1543.3 μg/ml and 820.4 μg/ml respectively. Conclusion. Based upon our results we suggest the use of Mc Couch et al. (1988 method with fresh leaftissue to obtain a high quality DNA suitable to be used with RAPDs molecular markers.

  16. Enhanced radiation response in radioresistant MCF-7 cells by targeting peroxiredoxin II

    Directory of Open Access Journals (Sweden)

    Diaz AJG


    Full Text Available Anthony Joseph Gomez Diaz,1 Daniel Tamae,2 Yun Yen,3 JianJian Li,4 Tieli Wang1 1Department of Chemistry and Biochemistry, California State University at Dominguez Hills, Carson, CA, 2Center of Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania, Philadelphia, PA, 3Department of Clinical and Molecular Pharmacology, Beckman Research Institute of City of Hope National Medical Center, Duarte, CA, 4Department of Radiation Oncology, University of California Davis, Sacramento, CA, USA Abstract: In our previous study, we identified that a protein target, peroxiredoxin II (PrxII, is overexpressed in radioresistant MCF+FIR3 breast-cancer cells and found that its expression and function is associated with breast-cancer radiation sensitivity or resistance. Small interference RNA (siRNA targeting PrxII gene expression was able to sensitize MCF+FIR3 radioresistant breast-cancer cells to ionizing radiation. The major focus of this work was to investigate how the radiation response of MCF+FIR3 radioresistant cells was affected by the siRNA that inhibits PrxII gene expression. Our results, presented here, show that silencing PrxII gene expression increased cellular toxicity by altering cellular thiol status, inhibiting Ca2+ efflux from the cells, and perturbing the intracellular Ca2+ homeostasis. By combining radiotherapy and siRNA technology, we hope to develop new therapeutic strategies that may have potential to enhance the efficacy of chemotherapeutic agents due to this technology's property of targeting to specific cancer-related genes. Keywords: siRNA, PrxII, radiation resistance, Ca2+, MCF+FIR3

  17. Screening for fetal chromosome abnormalities during the second trimester

    International Nuclear Information System (INIS)

    Objective: To develop a pre -natal screening program for fetal chromosome abnormalities based on risk values calculated from maternal serum markers levels during the second trimester. Methods: Serum levels of AFP, β-HCG, uE3 were determined with CLIA in 1048 pregnant women during 14-21w gestation period and the results were analyzed with a specific software (screening program for Down' s syndrome developed by Beckman) for the risk rate. In those women defined as being of high risk rate, cells from amniotic fluid or umbilical cord blood were studied for karyotype analysis. Results: Of these 1048 women, 77 were designated as being of high risk rate for several chromosome abnormalities i.e. Down's syndrome, open spina bifida and trisomy -18 syndrome (overall positive rate 7.3%). Further fetal chromosome study in 31 of them revealed three proven cases of abnormality. Another cord blood study was performed in a calculated low risk rate case but with abnormal sonographic finding at 31 w gestation and proved to be abnormal (software study false negative). The remaining 46 high risk rate cases either refused future study (n=35) or were lost for follow-up (n=11). Fortunately, all the 35 women refused further study gave birth to normal babies without any chromosome abnormalities discovered on peripheral blood study. Besides, in a trial study, five high risk rate women were again evaluated a few weeks later but with tremendous difference between the results. Conclusion: The present program proves to be clinically useful but needs further study and revision. Many factors may influence the result of the analysis and the duration of gestation period in weeks should be as accurate as possible. At present, in order to avoid getting false negatives, we don't advise a second check in 'high risk' cases. (authors)

  18. Determination of Ondansetron in Human Plasma by RP-HPLC%昂丹司琼人体血药浓度的RP-HPLC测定法

    Institute of Scientific and Technical Information of China (English)

    张尊建; 邹巧根; 李伟; 燕立波; 相秉仁; 吴如金


    目的:建立人血浆中昂丹司琼的RP-HPLC测定法,用于昂丹司琼片的人体药代动力学研究.方法:血浆样品加乙腈脱蛋白后,离心,取上清液,挥干,用少量流动相溶解,供进样分析.采用Alltima C18柱(4.6 mm×250 mm,5 μm),预柱:Beckman-ultrasphere ODS(4.6 mm×45 mm,5 μm),以醋酸钠缓冲液(pH=4.2)-乙腈(75∶ 25)为流动相,流速:1 mL*min-1,于310 nm波长处检测,灵敏度:0.000 1 AUFS. 测定了10名健康志愿者单剂量口服昂丹司琼8 mg后的血药浓度-时间过程.结果:最低检测限达0.5 ng*mL-1,线性范围为1~80 ng*mL-1(r=0.992 2),日内和日间精密度均小于10%,平均回收率为88.7%~98.2%,符合生物样品分析要求.结论:本法操作简单,准确,灵敏度高,可用于昂丹司琼片的药代动力学研究.

  19. 上海地区血液分析仪准确性的研究和探讨%Study on the accuracy of automated hematology analyzers in Shanghai

    Institute of Scientific and Technical Information of China (English)

    胡晓波; 李泳; 吴驾浦; 黄永华; 宋颖; 许蕾; 金大鸣; 张锦锋



  20. Characterization of Fricke dosimeter developed at Centro Regional de Ciencias Nucleares, Recife, PE, Brazil

    International Nuclear Information System (INIS)

    Fricke Xylenol Gel (FXG) is a modified Fricke dosimeter system that was tested at the Centro Regional de Ciencias Nucleares (CRCN/CNEN), in Recife, Brazil. The Fricke dosimeter system has been used in our laboratory successfully. We decided to test the FXG in our laboratory because this dosimetric system presents some advantages in relation to the Fricke dosimeter, such as: easy preparation, more sensitivity for doses between 5 - 30 Gy, good reproducibility, tissue equivalence and multidimensional dose information. The dosimetric solution was synthesized with 0.392 g of ferrous ammonium sulphate, 0.0060 g of sodium chloride, 0.0076 g of xylenol orange, 5.0 g of gelatine (270 bloom porcine) and 300 μl of sulphuric acid in 100 ml of water. First, the water-gelatin mixture was heated and continuously stirred with a magnetic stirrer while the others reagents were added and the temperature was raised to 45 deg C. Once the solution ready it was transferred to acrylic cuvettes and kept in dark under refrigeration at 5 deg C during 12 h. Characteristics such as absorption spectrum, stability and linearity were evaluated by spectrophotometry (Beckman DU-640 UV-visible spectrophotometer). The developed material presented a good performance: ferric ion concentration at 589 nm, after irradiation; linear dose-response curve between 5 and 30 Gy; and saturation region for doses higher than 30 Gy. One disadvantage of the FXG system is its poor storage stability, because the non-irradiated samples suffer a selfdegradation and this could be a limitation for the use of FXG system. (author)

  1. Rapid detection of acute kidney injury by urinary neutrophil gelatinase-associated lipocalin after cardiopulmonary bypass surgery

    International Nuclear Information System (INIS)

    Objective: To determine the accuracy of neutrophil gelatinase-associated lipocalin (NGAL) in early detection of acute kidney injury (AKI) after cardiopulmonary bypass (CPB) surgery by comparing with serum creatinine. Study Design: Descriptive study. Place and Duration of Study: Department of Chemical Pathology and Endocrinology, AFIP in collaboration with AFIC/ NIHD, Rawalpindi, from April to December 2011. Methodology: Eighty eight patients undergoing CPB surgery in AFIC/NIHD were included by consecutive sampling. Blood samples of subjects for serum creatinine analysis were drawn pre-operatively, 4 h, 24 h and 48 h after CPB surgery. Spot urine samples for NGAL were collected at 4 h after CPB surgery. Urine samples were analyzed on Abbott ARCHITECT i2000SR analyzer whereas serum creatinine samples were measured on Beckman UniCel DxC 600 Synchron Clinical System. Results: Out of 88 patients, 11 (13%) cases developed AKI 4 h postoperatively. Urinary NGAL increased markedly at 4 h postoperatively as compared to serum creatinine which showed rise at 24 - 48 h after cardiac surgery. Analysis of urine NGAL at a cutoff value of 87 ng/ml showed area under the curve of 0.91 [95% confidence interval (CI) 0.83 - 0.96] with sensitivity of 90.9% (95% CI 58.7 - 98.5) and specificity of 98.7% (95% CI 92.9-99.8). There was a positive correlation of 4 h urine NGAL and serum delta creatinine at 48 h, which was statistically significant (rs = 0.33, p = 0.001). Conclusion: The study demonstrated that levels of urine NGAL in patients suffering from AKI increased significantly at 4 h as compared to serum creatinine levels. Urine NGAL is an early predictive biomarker of AKI after CPB. (author)

  2. Comparison of urine with plasma neutrophil gelatinase-associated lipocalin in detecting acute kidney injury after cardiopulmonary bypass surgery

    International Nuclear Information System (INIS)

    Objective: To compare the accuracy of urine with plasma neutrophil gelatinase-associated lipocalin (NGAL) in early detection of acute kidney injury (AKI) following cardiopulmonary bypass (CPB) surgery. Study Design: A prospective cohort study. Place and duration of study: Department of Chemical Pathology and Endocrinology, AFIP from December 2011 to July 2012. Patients and Methods: Ninety three adult patients planned for CPB surgery in AFIC/NIHD were consecutively included. Blood for serum creatinine were collected preoperatively, 4, 24 and 48 hours (h) after CPB surgery. Blood and urine samples for NGAL analysis were collected only at 4 h. Serum creatinine, plasma and urine NGAL samples were analyzed on UniCel at the rate DxC 600 (Beckman), TRIAGE meter pro (Biosite) and ARCHITECT i2000SR analyzer (Abbott) respectively. Results: Out of 93 patients undergoing CPB surgery, 12 (13%) developed AKI. AKI patients had significantly higher median interquartile range (IQR) urine NGAL of 180 ng/ml (105-277 ng/ml) as compared to control of 6 ng/ml (2-15 ng/ml) and median plasma NGAL of 170 ng/ml (126-274 ng/ml) as compared to control of 75 ng/ml (61-131 ng/ml). The patients had increased urine vs plasma NGAL area under curve (AUC) ( 0.91 vs 0.70 (p = <0.001)), better sensitivity (91% vs 82%) and specificity (98% vs 65%). Conclusion: Plasma and urine NGAL values increased significantly in AKI patients as compared to serum creatinine values. Urine in comparison to plasma NGAL revealed more sensitivity and specificity in detecting AKI following CPB surgery. (author)

  3. Development of a homogeneous pulse shape discriminating flow-cell radiation detection system

    International Nuclear Information System (INIS)

    A homogeneous flow-cell radiation detection system which utilizes coincidence counting and pulse shape discrimination circuitry was assembled and tested with five commercially available liquid scintillation cocktails. Two of the cocktails, Ultima Flo (Packard) and Mono Flow 5 (National Diagnostics) have low viscosities and are intended for flow applications; and three of the cocktails, Optiphase HiSafe 3 (Wallac), Ultima Gold AB (Packard), and Ready Safe (Beckman), have higher viscosities and are intended for static applications. The low viscosity cocktails were modified with 1-methylnaphthalene to increase their capability for alpha/beta pulse shape discrimination. The sample loading and pulse shape discriminator setting were optimized to give the lowest minimum detectable concentration for methylnaphthalenein a 30 s count time. Of the higher viscosity cocktails, Optiphase HiSafe 3 had the lowest minimum detectable activities for alpha and beta radiation, 0.2 and 0.4 Bq/ml for 233U and 90Sr/90Y, respectively, for a 30 s count time. The sample loading was 70% and the corresponding alpha/beta spillover was 5.5%. Of the low viscosity cocktails, Mono Flow 5 modified with 2.5% (by volume) 1-methylnaphthalene resulted in the lowest minimum detectable activities for alpha and beta radiation; 0.3 and 0.5 Bq/ml for 233U and 90Sr/90Y, respectively, for a 30 s count time. The sample loading was 50%, and the corresponding alpha/beta spillover was 16.6%. HiSafe 3 at a 10% sample loading was used to evaluate the system under simulated flow conditions

  4. Utilization of 15N-Diammonium Phosphate by Ruminants to Produce Milk and Meat Proteins

    International Nuclear Information System (INIS)

    The authors investigated the alimentary role of diammonium phosphate (DAP) in ruminants. For this study DAP labelled with 15N was used; analysis of the 15N atomic per cent excess was made with an Italelettronica mass spectrophotometer (model SP 21 F) and the amino acid determination by a Beckman-Spinco amino acid analyser (model 120B) fitted with a preparative column. For the experiment 7 g of DAP at 15 and 20 at. % excess 15N were administered once to mature lactating and non-lactating sheep, respectively. The measurement of 15N in the protein and isolated amino acids of milk and meat showed: (1) The milk protein produced in the first 24 h contained the highest atomic per cent excess of 15SN, 0.093; (2) That the supplemental 15N was found in all the amino acids of milk proteins except tryptophane. The atomic per cent excess of 15N was observed to vary between the various amino acids. These results confirmed previous observations on bacterial protein synthesized from DAP. (3) Muscle protein 15N maximized on the third day after administration of the 15N-DAP, with an atomic per cent excess of 0.040; (4) The atomic per cent excess of 15N in the individual amino acids of muscle protein is significant in only two amino' acids, serine and cystine; and (5) That after 8 d of adaptation there are no traces of DAP in milk or meat proteins, urine or faeces. The authors conclude that the ruminant, after a period of adaptation and through the mediation of ruminant microorganisms, is able to use the nitrogen of diammonium phosphate for the synthesis of milk and meat proteins. (author)

  5. Annual symposium on Frontiers in Science

    Energy Technology Data Exchange (ETDEWEB)

    Metzger, N.; Fulton, K.R.


    This final report summarizes activities conducted for the National Academy of Sciences' Annual Symposium on Frontiers of Science with support from the US Department of Energy for the period July 1, 1993 through May 31, 1998. During the report period, five Frontiers of Science symposia were held at the Arnold and Mabel Beckman Center of the National Academies of Sciences and Engineering. For each Symposium, an organizing committee appointed by the NAS President selected and planned the eight sessions for the Symposium and identified general participants for invitation by the NAS President. These Symposia accomplished their goal of bringing together outstanding younger (age 45 or less) scientists to hear presentations in disciplines outside their own and to discuss exciting advances and opportunities in their fields in a format that encourages, and allows adequate time for, informal one-on-one discussions among participants. Of the 458 younger scientists who participated, over a quarter (124) were women. Participant lists for all symposia (1993--1997) are attached. The scientific participants were leaders in basic research from academic, industrial, and federal laboratories in such disciplines as astronomy, astrophysics, atmospheric science, biochemistry, cell biology, chemistry, computer science, earth sciences, engineering, genetics, material sciences, mathematics, microbiology, neuroscience, physics, and physiology. For each symposia, the 24 speakers and discussants on the program were urged to focus their presentations on current cutting-edge research in their field for a scientifically sophisticated but non-specialist audience, and to provide a sense of the experimental data--what is actually measured and seen in the various fields. They were also asked to address questions such as: What are the major research problems and unique tools in their field? What are the current limitations on advances as well as the frontiers? Speakers were asked to provide a

  6. Use of the selected overlap LIDAR experiment (SOLEX) system with the 248 nm krypton fluoride and the 355 nm neodymium:yttrium aluminum garnet lasers for the calibration of LIDAR systems for water vapor determination (United States)

    Mensah, Francis Emmanuel Tofodji

    Water vapor is one of the most important atmospheric variables that play a key role in air quality, global warming, climate change and hurricane formation. In this dissertation, use was made of two laser systems, the 248-nm KrF laser and the 355 nm Nd-YAG laser, with the use of Raman scattering to measure water vapor in the atmosphere. These two systems have been calibrated more accurately, using the LIDAR approach named SOLEX (Selected Overlap LIDAR Experiment). All the experiments were carried out at the Howard University Beltsville campus located on a 107 acre research site, at Beltsville, MD, 15 miles from downtown Washington DC, near the National Agricultural Research Center (NARC), and the NASA Goddard Space Flight Center (GSFC). The geographical coordinates are: 39°04.01'N latitude, and 76°52.31'W longitude. The receiver system used during these experiments is a 30" (76.2 cm), f/ 9 Cassegranian telescope, while the detector system uses a prism spectrometer (Beckman), with a 2-meter, double-fold optical path and a variable slit width is placed at the image plane of the telescope. With the use of the SOLEX system, this dissertation provides an accurate calibration of the two LIDAR Systems for water vapor measurement in the troposphere at the following ranges: 83.7 ft, 600 ft, 800 ft, 1000 ft and 1080 ft. Data analysis shows a pretty high sensitivity of the LIDAR system for water vapor measurement and the efficiency of the SOLEX method.

  7. Use of an external source (60Co) for 32P detection efficiency determination by the Cerenkov effect, in soil extracts

    International Nuclear Information System (INIS)

    The detection of 32P in aqueous extracts is usually made with the aid of a Geiger-Muller detector, with thin window and sample on a planchet. Presently the technique is being developed of detection of high energy beta particles emitters (32P, 42K, 86Rb) through the Cerenkov effect, using a commercial liquid scintillation system. This technique, despite being approximately 30 times more sensitive, has the inconvenience of varying the detection efficiency, mainly for color samples (soil extracts, for instance). From this stems the need for determining the detection efficiency for each sample. The internal standardization and channels ratio methods show a series of drawbacks, mainly the non-reutilization of the samples (1st method) and statistical uncertainty for low activity samples (2nd method). The elimination of these dreawbacks can be achieved through the utilization of the external standardization method. A 60Co source with 1,4 μCi activity has been adapted to the sample elevator of the detector system, and a comparison was made with the channels ratio method to evaluate the efficiency of 32P detection in soil extracts (P extraction and fractionation). The external standardization method showed to be more accurate, besides being influenced to a lesser degree by high voltage variation, sample volume and vial types. In the case of large samples, it is advisable to carry out detection in vials filled up to their full capacity; in the case of small samples, the whole volume should be transferred to the vials and completed up to 9 ml for nylon vials,10 ml for glass vials and 11 to 14 ml for polyethilene vials. On the other hand, plastic vials showed higher detection efficiency than ones. As to background radiation, the lowest rates were given by nylon vials and the highest by Beckman glass vials

  8. [Effects of ruta SSP on the activity of the smooth gastrointestinal muscle isolated of rat]. (United States)

    Grigorjev, Carlota A; Brizuela, Nilda Y


    Ruta graveolens L. and Ruta chalepensis L. are plants used in folkloric medicine as antispasmodics, digestive and for intestinal gases. Animals used as experimental model were rats of the Wistar line, adult females, clinically healthy and with a weight average of 250 grams. We used strips of stomach and duodenum. Each one of the segments mounted on two stirrups in a bath of organ isolated with Ringer-lactate solution, at 37° C , pH: 7.3-7.4, and bubbled with 95% O2, 5% CO2. One of the stirrups was connected vertically to the bottom of the bath and the other to a transducer of tension connected to a Beckman polygraph. We applied 500 mgs of basal tension. After the stabilization, the ethanolic extract of Ruta ssp was added in increasing doses. At 50μl/ml the tone lower 23% in small intestine and lower 27% in stomach. However at 100 μl/ml the tone lower 32% and 35% respectively. In the other parameters the amplitude decrease 50% in the stomach at dose of 5 μl/ml while in the small intestine the amplitude lower 60%. With 10 μl/ml the amplitude change in both organs ( 96% in small intestine, and 75% in stomach). The frequency changes in both organs ( 32% in small intestine, and 50% in stomach) at 10 μl/ml Rue showed decreased effects on isolated small intestine and stomach were is dose dependent, maybe we were demonstrated the effects digestive of Ruta. PMID:21450144

  9. Dispersion relations of cytoskeleton dynamics

    Directory of Open Access Journals (Sweden)

    Wang R


    Full Text Available Ru Wang,1,2 Lei Lei,3 Shamira Sridharan,1,3 Yingxiao Wang,3 Alex J Levine,4,5 Gabriel Popescu,1,3,6 1Quantitative Light Imaging Laboratory, Beckman Institute for Advanced Science and Technology, 2Department of Mechanical Science and Engineering, 3Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 4Department of Chemistry and Biochemistry, 5Department of Physics and Astronomy, University of California, Los Angeles, CA, 6Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA Abstract: While it is well known that the cytoskeleton plays a fundamental role in maintaining cell shape, performing cell division, and intracellular transport, its spatiotemporal dynamics are insufficiently understood. The dispersion relation, which is fundamental for understanding the connection between spatial and temporal scales of a dynamic system, was employed here for the first time to study the activity of actin and microtubules. Using green fluorescence protein for time-lapse imaging of the cytoskeleton, we showed that the dispersion relation can distinguish between diffusive and active transport of actin and microtubule filaments. Our analysis revealed that along the filaments, the transport was deterministic, as one might expect as the result of the active polymerization process, while across the filaments diffusion was dominant. Furthermore, using drugs to block the polymerization–depolymerization of both actin and microtubules, we measured that the transport immediately became diffusive, as expected. However, unexpectedly, our results indicated that within a few minutes from blocking its polymerization, actin recovered an active transport component. This deterministic component vanished upon treatment with nocodazole, indicating that fragments of actin were actively transported along microtubules. Because it provides information over broad temporal and spatial scales

  10. A new concept for combisystems characterization. The FSC method

    Energy Technology Data Exchange (ETDEWEB)

    Letz, Thomas [INES - Education, Parc Technologique de Savoie Technolac, 50 avenue du Leman BP 258, F-73 375 Le Bourget du Lac Cedex (France); Bales, Chris [Solar Energy Research Center SERC, School of Industrial Technology and Management, Hoegskolan Dalarna, S-78188 Borlaenge (Sweden); Perers, Bengt [DTU Civil Engineering, Technical University of Denmark, Brovej, Building 118, DK-2800 Kgs. Lyngby (Denmark)


    Solar combisystems are relatively complex systems with many different components and operational parameters. Before the beginning of IEA-SHC Task 26 ('solar combisystems'), no method was available with which they could be compared. The well known 'f-chart' method was introduced by Duffie and Beckman already in the seventies, but was only useful for dimensioning generic combisystems, with a defined hydraulic scheme. It didn't give a method to compare different designs. The objective of this work was to develop a simple tool for characterizing the performance of these systems. The method used was to analyse the comprehensive simulation results of Task 26 and to look for relationships between the key external factors of climate and load, and the system performance. The result is a new and simple methodology for characterization of solar combisystems, called the fractional solar consumption (FSC) method. FSC is a dimensionless quantity, which takes simultaneously into account the climate, the space heating and domestic hot water loads, the collector size, its orientation and tilt angle, but which does not depend on the studied system design. The study shows that fractional energy savings, with and without parasitic energy included, can be expressed as a quadratic function of FSC. The relationship was shown to be valid for a wide range of conditions, but to be limited for certain parameters such as collector orientation and hot water load. The method has been used to create a nomogram and the computer design tool CombiSun. (author)

  11. Measurement of isotopic uranium in water for compliance monitoring by liquid scintillation counting with alpha/beta discrimination

    International Nuclear Information System (INIS)

    A simple and inexpensive method is described for analysis of uranium (U) activity and mass in water by liquid scintillation counting using α/β discrimination. This method appears to offer a solution to the need for an inexpensive protocol for monitoring U activity and mass simultaneously and an alternative to the potential inaccuracy involved when depending on the mass-to-activity conversion factor or activity screen. U is extracted virtually quantitatively into 20 ml extractive scintillator from a 1-ell aliquot of water acidified to less than pH 2. After phase separation, the sample is counted for a 20-minute screening count with a minimum detection level of 0.27 pCi ell -1. α-particle emissions from the extracted U are counted with close to 100% efficiency with a Beckman LS6000 LL liquid scintillation counter equipped with pulse-shape discrimination electronics. Samples with activities higher than 10 pCi ell -1 are recounted for 500-1000 minutes for isotopic analysis. Isotopic analysis uses events that are automatically stored in spectral files and transferred to a computer during assay. The data can be transferred to a commercially available spreadsheet and retrieved for examination or data manipulation. Values for three readily observable spectral features can be rapidly identified by data examination and substituted into a simple formula to obtain 234U/238U ratio for most samples. U mass is calculated by substituting the isotopic ratio value into a simple equation. The utility of this method for the proposed compliance monitoring of U in public drinking water supplies was field tested with a survey of drinking water from Texas supplies that had previously been known to contain elevated levels of gross α activity. U concentrations in 32 samples from 27 drinking water supplies ranged from 0.26 to 65.5 pCi ell -1, with seven samples exceeding the proposed Maximum Contaminant Level

  12. Frameshift Deletion by Sulfolobus solfataricus P2 DNA Polymerase Dpo4 T239W Is Selective for Purines and Involves Normal Conformational Change Followed by Slow Phosphodiester Bond Formation* (United States)

    Zhang, Huidong; Beckman, Jeff W.; Guengerich, F. Peter


    The human DNA polymerase κ homolog Sulfolobus solfataricus DNA polymerase IV (Dpo4) produces “−1” frameshift deletions while copying unmodified DNA and, more frequently, when bypassing DNA adducts. As judged by steady-state kinetics and mass spectrometry, bypass of purine template bases to produce these deletions occurred rarely but with 10-fold higher frequency than with pyrimidines. The DNA adduct 1,N2-etheno-2′-deoxyguanosine, with a larger stacking surface than canonical purines, showed the highest frequency of formation of −1 frameshift deletions. Dpo4 T239W, a mutant we had previously shown to produce fluorescence changes attributed to conformational change following dNTP binding opposite cognate bases (Beckman, J. W., Wang, Q., and Guengerich, F. P. (2008) J. Biol. Chem. 283, 36711–36723), reported similar conformational changes when the incoming dNTP complemented the base following a templating purine base or bulky adduct (i.e. the “+1” base). However, in all mispairing cases, phosphodiester bond formation was inefficient. The frequency of −1 frameshift events and the associated conformational changes were not dependent on the context of the remainder of the sequence. Collectively, our results support a mechanism for −1 frameshift deletions by Dpo4 that involves formation of active complexes via a favorable conformational change that skips the templating base, without causing slippage or flipping out of the base, to incorporate a complementary residue opposite the +1 base, in a mechanism previously termed “dNTP-stabilized incorporation.” The driving force is attributed to be the stacking potential between the templating base and the incoming dNTP base. PMID:19837980

  13. Frameshift deletion by Sulfolobus solfataricus P2 DNA polymerase Dpo4 T239W is selective for purines and involves normal conformational change followed by slow phosphodiester bond formation. (United States)

    Zhang, Huidong; Beckman, Jeff W; Guengerich, F Peter


    The human DNA polymerase kappa homolog Sulfolobus solfataricus DNA polymerase IV (Dpo4) produces "-1" frameshift deletions while copying unmodified DNA and, more frequently, when bypassing DNA adducts. As judged by steady-state kinetics and mass spectrometry, bypass of purine template bases to produce these deletions occurred rarely but with 10-fold higher frequency than with pyrimidines. The DNA adduct 1,N(2)-etheno-2'-deoxyguanosine, with a larger stacking surface than canonical purines, showed the highest frequency of formation of -1 frameshift deletions. Dpo4 T239W, a mutant we had previously shown to produce fluorescence changes attributed to conformational change following dNTP binding opposite cognate bases (Beckman, J. W., Wang, Q., and Guengerich, F. P. (2008) J. Biol. Chem. 283, 36711-36723), reported similar conformational changes when the incoming dNTP complemented the base following a templating purine base or bulky adduct (i.e. the "+1" base). However, in all mispairing cases, phosphodiester bond formation was inefficient. The frequency of -1 frameshift events and the associated conformational changes were not dependent on the context of the remainder of the sequence. Collectively, our results support a mechanism for -1 frameshift deletions by Dpo4 that involves formation of active complexes via a favorable conformational change that skips the templating base, without causing slippage or flipping out of the base, to incorporate a complementary residue opposite the +1 base, in a mechanism previously termed "dNTP-stabilized incorporation." The driving force is attributed to be the stacking potential between the templating base and the incoming dNTP base. PMID:19837980

  14. A fast radiochemical procedure to measure Tc-99 in environmental samples and gaseous and liquid effluents

    International Nuclear Information System (INIS)

    Liquid effluent samples are collected in polyethylene bottles. Sampling in the gaseous effluents relies on conventional NaOH scrubbers. No noticeable adsorption on the vessel walls takes place in HNO3/H2O2 and NaOH, respectively, in the plastic botteles used. A known amount of Tc-95m is added in order to determine the chemical yield prior to the analysis. The pertechnetate in the samples is first fixed on an anionexchanger. Following elution with perchlorate/sulfite solution TC is coprecipitated with iron(II) hydroxide. Having dissolved the precipitation in H2O2/H2SO4 one extracts with TBP/alkane. To achieve a better decontamination from other nuclides a re-extraction is performed with NaOH and after acidification with H2SO4 a further extraction using TBP/Alkane. Before the measurement of the sample the alkane is eliminated in a rotation evaporator and the remaining TBP is dissolved directly in the liquid scintillator. Other beta emitters generated as fission products in the course of nuclear fission are satisfactorily separated during the analysis. The decontamination factors for I, Se, Te, Sb, and Ru are > 105. The measurements are carried out with a Beckman LS 7800 instrument. The optimum channel setting is found from the spectra of the two nuclides Tc-95m and Tc-99. The quenching plots are determined accordingly. The measurements are evaluated with a table computer. Chemical yields of 50-90% are achieved. The detection limit for 600 minutes of measuring time is 1 pCi per sample. One person is capable of performing about 15 analyses per week using the method described. (orig./HP)

  15. Growth and radiosensitivity of irradiated human glioma cell progeny

    Institute of Scientific and Technical Information of China (English)

    Chao Li; Li Li; Changshao Xu; Juying Zhou


    BACKGROUND: Progenitors of the immortalized human glioma cell line, SHG-44, are significantly less sensitive to irradiation. Two hypotheses regarding the mechanism of this effect exist: several studies have suggested that there is a subgroup with different radiosensitivities in identical cell group, and the progenitors of irradiate is a adaptive response subgroup, so its radiosensitivity is descend. A second hypothesis suggests that irradiated glioma progeny have a stronger ability to repair DNA damage. This would suggest that when progeny are continuously irradiated, resistance to irradiation-induced DNA increases, and radiosensitivity decreases.OBJECTIVE: To investigate radiosensitivity and growth features after irradiation to progeny of the human glioma cell line SHG-44.DESIGN, TIME AND SETTING: A randomized, controlled experiment, which was performed at the Department of Radiology Laboratory, the First Hospital Affiliated to Soochow University, between September 2004 and January 2006.MATERIALS: The glioma cell line SHG-44 was provided by the Institute of Neuroscience, First Affiliated Hospital of Suzhou University. Propidium iodide reagent was provided by Coulter Corporation. A linear accelerator, KD-2 type, was provided by Siemens, Germany. The flow cytometer EPICS-XL was provided by Coulter Corporation.METHODS: Brain glioma SHG-44 cells were divided into four groups: SHG-44, SHG-44-2, SHG-44-6, and SHG-44-10. The SHG-44-2, SHG-44-6, and SHG-44-10 cells were vertically irradiated with varying doses of 2,6 and 10 Gy by a linear accelerator (6 MVX). The cells were passaged for 15 generations and cultured in RPMI-1640 culture media.MAIN OUTCOME MEASURES: Community re-double time, mean lethal dose (D0), extrapolation number (N), fraction surviving fraction irradiated by 2 Gy dose (SF2), quasi-threshold dose (Dq), and cell cycle.RESULTS: The Population doubling time (PDT) of SHG-44-2, SHG-44-6, and SHG-44-10 cell groups was not significant (P=0.052). Compared to

  16. Sources of SOA gaseous precursors in contrasted urban environments: a focus on mono-aromatic compounds and intermediate volatility compounds (United States)

    Salameh, Therese; Borbon, Agnès; Ait-Helal, Warda; Afif, Charbel; Sauvage, Stéphane; Locoge, Nadine; Bonneau, Stéphane; Sanchez, Olivier


    , SP95 E10, and SP98) and was used to constraint evaporative emissions in order to predict the headspace vapour composition (Harley and Coulter-Burke, 2000). Modelled and observed compositions are in good agreement (differences up to 20%). Therefore, the implemented model is a relevant tool to test the sensitivity of BTEX and other VOCs ambient composition to evaporative emissions of fuels with regards to their composition. Such analysis will be extended to other target cities and similarities/differences will be presented regarding regional characteristics. This work was supported by the Ile de France region, Life and PHOTOPAQ grant, PICS-CNRS, ENVIMED and ChArMEx. We would like to thank Laurence Dépelchin and Thierry Léonardis for technical support and AIRPARIF for providing the data. Borbon, A., et al. (2013) Emission ratios of anthropogenic VOC in northern mid-latitude megacities: observations vs. emission inventories in Los Angeles and Paris, J. Geophys. Res. 118, 2041 - 2057. Harley, R. and Coulter-Burke, S. (2000) Relating Liquid Fuel and Headspace Vapor Composition for California Reformulated Gasoline Samples Containing Ethanol, Environ. Sci. Technol. 34, 4088-4094. Ait-Helal, W.; Borbon, A.; Sauvage, S.; et al., Atmos. Chem. Phys. vol. 14 , No. 19 , p. 10439-10464

  17. The Putative Role of the Non-Gastric H+/K+-ATPase ATP12A (ATP1AL1 as Anti-Apoptotic Ion Transporter: Effect of the H+/K+ ATPase Inhibitor SCH28080 on Butyrate-Stimulated Myelomonocytic HL-60 Cells

    Directory of Open Access Journals (Sweden)

    Martin Jakab


    Full Text Available Background/Aims: The ATP12A gene codes for a non-gastric H+/K+ ATPase, which is expressed in a wide variety of tissues. The aim of this study was to test for the molecular and functional expression of the non-gastric H+/K+ ATPase ATP12A/ATP1AL1 in unstimulated and butyrate-stimulated (1 and 10 mM human myelomonocytic HL-60 cells, to unravel its potential role as putative apoptosis-counteracting ion transporter as well as to test for the effect of the H+/K+ ATPase inhibitor SCH28080 in apoptosis. Methods: Real-time reverse-transcription PCR (qRT-PCR was used for amplification and cloning of ATP12A transcripts and to assess transcriptional regulation. BCECF microfluorimetry was used to assess changes of intracellular pH (pHi after acute intracellular acid load (NH4Cl prepulsing. Mean cell volumes (MCV and MCV-recovery after osmotic cell shrinkage (Regulatory Volume Increase, RVI were assessed by Coulter counting. Flow-cytometry was used to measure MCV (Coulter principle, to assess apoptosis (phosphatidylserine exposure to the outer leaflet of the cell membrane, caspase activity, 7AAD staining and differentiation (CD86 expression. Results: We found by RT-PCR, intracellular pH measurements, MCV measurements and flow cytometry that ATP12A is expressed in human myelomonocytic HL-60 cells. Treatment of HL-60 cells with 1 mM butyrate leads to monocyte-directed differentiation whereas higher concentrations (10 mM induce apoptosis as assessed by flow-cytometric determination of CD86 expression, caspase activity, phosphatidylserine exposure on the outer leaflet of the cell membrane and MCV measurements. Transcriptional up-regulation of ATP12A and CD86 is evident in 1 mM butyrate-treated HL-60 cells. The H+/K+ ATPase inhibitor SCH28080 (100 µM diminishes K+-dependent pHi recovery after intracellular acid load and blocks RVI after osmotic cell shrinkage. After seeding, HL-60 cells increase their MCV within the first 24 h in culture, and subsequently

  18. 两种微生物学等级老龄Wistar大鼠血清生化值比较%Comparison of serum biochemistry between specific pathogen-free and coventional aged Wistar rats

    Institute of Scientific and Technical Information of China (English)

    肖育华; 詹纯列; 李建军; 武婕; 李新春; 郑文岭


    目的探讨普通级、SPF级老龄Wistar大鼠血清生化值的差异.方法用Coulter-JT全自动生化检测仪检测血清生化值.结果在老年SPF级Wistar大鼠的雌性与雄性之间,谷丙酶、总蛋白、碱性磷酸酶、总胆固醇、甘油三酯、尿素氮、肌酐、铁、磷、血糖、尿酸、低密度脂蛋白差异非常显著(P<0.01),白蛋白、乳酸脱氢酶、载脂蛋白B差异显著(P<0.05).老年SPF级雄性Wistar大鼠与老年普通级雄性Wistar大鼠间的总蛋白、白蛋白、白球比、总胆固醇、甘油三酯、血糖、载脂蛋白A1、载脂蛋白B、尿酸、高密度脂蛋白、低密度脂蛋白、谷草酶差异非常显著(P<0.01),谷丙酶、铁、镁差异显著(P<0.05).老年SPF级雌性Wistar大鼠与普通级雌性Wistar大鼠间的谷丙酶、总蛋白、白蛋白、白球比、碱性磷酸酶、甘油三酯、尿素氮、肌酐、铁、载脂蛋白A1、载脂蛋白B、高密度脂蛋白、低密度脂蛋白、胆汁酸差异非常显著(P<0.01),镁含量差异显著(P<0.05).结论微生物学等级因素对Wistar大鼠的血清生化值有影响.%Objective To investigate the differences in serum biochemistry between specific pathogen-free (SPF) and conventional aged Wistar rats. Methods Coulter-JT Analyzer was used to measure the values of serum biochemistry in the two grades of rats. Results The serum levels of alanine aminotransferase (ALT), total protein (TP), alkaline phosphatase (ALP), total cholesterol (TC), triglyceride (TG), blood urea nitrogen (BUN), creatinine, Fe, P, glucose, uric acid (UA), and low density lipoprotein (LDL) were very significantly different between male and female Wistar rats of either conventional or SPF grade (P<0.01), which also had significant difference in albumin, lactate dehydrogenase (LDH) and apolipoprotein B (ApoB) (P<0.05).Between male aged Wistar rats of the two grades, the differences of TP, albumin, albumin/globulin (A/G) ratio, TC, TG, blood

  19. Impedance microflow cytometry for viability studies of microorganisms (United States)

    Di Berardino, Marco; Hebeisen, Monika; Hessler, Thomas; Ziswiler, Adrian; Largiadèr, Stephanie; Schade, Grit


    Impedance-based Coulter counters and its derivatives are widely used cell analysis tools in many laboratories and use normally DC or low frequency AC to perform these electrical analyses. The emergence of micro-fabrication technologies in the last decade, however, provides a new means of measuring electrical properties of cells. Microfluidic approaches combined with impedance spectroscopy measurements in the radio frequency (RF) range increase sensitivity and information content and thus push single cell analyses beyond simple cell counting and sizing applications towards multiparametric cell characterization. Promising results have been shown already in the fields of cell differentiation and blood analysis. Here we emphasize the potential of this technology by presenting new data obtained from viability studies on microorganisms. Impedance measurements of several yeast and bacteria strains performed at frequencies around 10 MHz enable an easy discrimination between dead and viable cells. Moreover, cytotoxic effects of antibiotics and other reagents, as well as cell starvation can also be monitored easily. Control analyses performed with conventional flow cytometers using various fluorescent dyes (propidium iodide, oxonol) indicate a good correlation and further highlight the capability of this device. The label-free approach makes on the one hand the use of usually expensive fluorochromes obsolete, on the other hand practically eliminates laborious sample preparation procedures. Until now, online cell monitoring was limited to the determination of viable biomass, which provides rather poor information of a cell culture. Impedance microflow cytometry, besides other aspects, proposes a simple solution to these limitations and might become an important tool for bioprocess monitoring applications in the biotech industry.

  20. A comparative study of hematological parameters of α and β thalassemias in a high prevalence zone: Saudi Arabia (United States)

    Mehdi, Syed Riaz; Al Dahmash, Badr Abdullah


    BACKGROUND AND AIMS: Saudi Arabia falls in the high prevalent zone of αα and β thalassemias. Early screening for the type of thalassemia is essential for further investigations and management. The study was carried out to differentiate the type of thalassemia based on red cell indices and other hematological parameters. MATERIALS AND METHODS: The study was carried out on 991 clinically suspected cases of thalassemias in Riyadh, Saudi Arabia. The hematological parameters were studied on Coulter STKS. Cellulose acetate hemoglobin electrophoresis and high-performance liquid chromatography (HPLC) were performed on all the blood samples. Gene deletion studies were carried out by restriction fragment length polymorphism (RFLP) technique using the restriction endonucleases Bam HI. STATISTICAL ANALYSIS: Statistical analysis was performed on SPSS 11.5 version. RESULTS: The hemoglobin electrophoresis and gene studies revealed that there were 406 (40.96%) and 59 (5.95 %) cases of β thalassemia trait and β thalassemia major respectively including adults and children. 426 cases of various deletion forms of α thalassemias were seen. Microcytosis was a common feature in β thalassemias trait and (-α/-α) and (--/αα) types of α thalassemias. MCH was a more significant distinguishing feature among thalassemias. β thalassemia major and α thalassemia (-α/αα) had almost normal hematological parameters. CONCLUSION: MCV and RBC counts are not statistically significant features for discriminating between α and β thalassemias. There is need for development of a discrimination index to differentiate between α and β thalassemias traits on the lines of discriminatory Indices available for distinguishing β thalassemias trait from iron deficiency anemia. PMID:22345994

  1. Effect of caffeine on gamma-ray induced G2 arrest in well-synchronized Chinese hamster ovary cells in vitro

    International Nuclear Information System (INIS)

    G1-rich cells were separated from exponentially growing asynchronous cultured Chinese hamster ovary (CHO-K1) cells by centrifugal elutriation and a Coulter Counter. The G1-rich cells were incubated in medium that contained hydroxyurea (HU) to kill S phase cells and obtain the purest G1/S boundary cells possible. The HU-treated cells were washed, and were again incubated, in medium without HU, to allow these well-synchronized G1/S boundary cells to progress to S and G2/M phases. At various times after release from G1/S boundary, 4 Gy of gamma-ray and/or caffeine was administered to the cells. Eight hours after the removal of HU, cell-cycle analysis was performed with a flow cytometer. G2 arrest induced by gamma-rays was clearly shown when radiation was given earlier than 6.5 hours after HU removal. G2 arrest induced by radiation given 0.5-6.5 hours after HU removal was reduced by caffeine treatment given 6.0-6.5 hours after HU removal. Caffeine released radiation-induced G2 arrest when the radiation was given before the cultured cells entered G2/M phase and when caffeine was applied to the irradiated cells at the time when G1/S boundary cells not treated by radiation or with caffeine entered G2/M phase. Our method of centrifugal elutriation combined with incubation with HU was useful for isolating pure G1/S boundary cells from in vitro asynchronous cultures. (author)

  2. The Origin and Age of Scallop Floodplain Benches from Difficult Run, Fairfax County, Virginia. (United States)

    Scamardo, J. E.; Pizzuto, J. E.; Skalak, K.; Benthem, A.


    Sediment is deposited within scallop-shaped erosional scarps that form between trees armoring the banks of Difficult Run, a suburban watershed with a forested riparian zone. These deposits create small (surface area 85 m2, volume 300 m3), low-lying floodplain landforms this group terms Scallop Floodplain Benches (SFB). It is hypothesized that SFB formed within the past couple decades initially forming as transversal accretion deposits and eventually gaining floodplain features dominated by vertical accretion. Stratigraphic data supports that SFB deposits begin laterally as sand and gravel bars approximately 100 cm thick, and continue to grow by vertical accretion of sand, silt, and clay. As a SFB reaches its maximum height, a distinctive levee develops adjacent to the channel, and fine-grained silt and clay are deposited behind the levee. Core samples to a depth of 118 cm and additional samples from an overbank event that occurred on June 20, 2015 were collected from one of two SFB on Difficult Run near Leesburg Pike. The grain size distribution was measured using a Coulter Counter and activities of Pb-210, Cs-137, and Be-7 were measured using High Purity Germanium Detectors. Cs-137 activities are relatively constant with depth without a well-defined peak, suggesting that the SFB was deposited after 1963. Be-7 is present in the recent flood deposits, but is absent below the surface, suggesting that the SFB deposits are at least several years old. Excess Pb-210 activities decrease exponentially with depth, and can be fit using the Constant Rate of Supply method to determine an average age of approximately 13.5 years for the SFB. The SFB is storing sediment at a rate of 27 tons/year, which is equal to 0.35% of the annual sediment load of Difficult Run, based on this average age. SFB appear to be a significant component of the sediment storage of Difficult Run and therefore should be considered in the sediment budget.

  3. Cell diameter measurements obtained with a handheld cell counter could be used as a surrogate marker of G2/M arrest and apoptosis in colon cancer cell lines exposed to SN-38

    Energy Technology Data Exchange (ETDEWEB)

    Tahara, Makiko [Oncogene Research Unit/Cancer Prevention Unit, Tochigi Cancer Center Research Institute, Utsunomiya, Tochigi (Japan); Department of Gastrointestinal Surgery, Jichi Medical University, Shimotsuke, Tochigi (Japan); Inoue, Takeshi [Oncogene Research Unit/Cancer Prevention Unit, Tochigi Cancer Center Research Institute, Utsunomiya, Tochigi (Japan); Miyakura, Yasuyuki; Horie, Hisanaga; Yasuda, Yoshikazu [Department of Gastrointestinal Surgery, Jichi Medical University, Shimotsuke, Tochigi (Japan); Fujii, Hirofumi [Division of Clinical Oncology, Jichi Medical University, Shimotsuke, Tochigi (Japan); Kotake, Kenjiro [Department of Surgery, Tochigi Cancer Center, Utsunomiya, Tochigi (Japan); Sugano, Kokichi, E-mail: [Oncogene Research Unit/Cancer Prevention Unit, Tochigi Cancer Center Research Institute, Utsunomiya, Tochigi (Japan)


    Highlights: •Chemo-sensitivity to SN-38 was assayed by the automated cell counter. •Colon cancer cell line, HCT116 cells were more sensitive to SN-38 than HT29 cells. •Increase of cell size reflects G2/M arrest. •Appearance of small particles indicates cell apoptosis. -- Abstract: In vitro assessment of chemosensitivity are important for experiments evaluating cancer therapies. The Scepter 2.0 cell counter, an automated handheld device based on the Coulter principle of impedance-based particle detection, enables the accurate discrimination of cell populations according to cell size and volume. In this study, the effects of SN-38, the active metabolite of irinotecan, on the colon cancer cell lines HCT116 and HT29 were evaluated using this device. The cell count data obtained with the Scepter counter were compared with those obtained with the {sup 3}H-thymidine uptake assay, which has been used to measure cell proliferation in many previous studies. In addition, we examined whether the changes in the size distributions of these cells reflected alterations in the frequency of cell cycle arrest and/or apoptosis induced by SN-38 treatment. In our experiments using the Scepter 2.0 cell counter, the cell counts were demonstrated to be accurate and reproducible measure and alterations of cell diameter reflected G2/M cell cycle arrest and apoptosis. Our data show that easy-to-use cell counting tools can be utilized to evaluate the cell-killing effects of novel treatments on cancer cells in vitro.

  4. Macronutrients leaf contents of corn in intercropping with forages of genus Panicum and Urochloa in simultaneous seeding

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    Denise Tsuzukibashi


    Full Text Available The planting of crops in degraded pasture areas is a formula used for decades by farmers to recover the productive capacity of pastures and soils. The integrated crop-livestock (ICL consists of different production systems of grains, fibers, wood, meat, milk and agro-deployed in the same area, in intercrop, rotation or succession. Typically this integration mainly involves the planting of grain and pasture in the recovery or deployment. This work aimed to evaluate the macronutrients leaf contents of irrigated corn intercropped with forages of the genus Panicum and Urochloa simultaneously to sown corn. The experiment was conducted at the Farm for Teaching, Research and Extension, Faculty of Engineering - UNESP, Ilha Solteira in an Oxisol in Savannah conditions, in experimental area that had a history of no-tillage to 8 years (previous crop corn. The experimental design used was randomized blocks with four replications and five treatments: Panicum maximum cv. Tanzania sown simultaneously (CTS corn; Panicum maximum cv. Mombaça sown simultaneously (CMS to corn; Urochloa brizantha cv. Xaraes sown simultaneously (CBS corn; Urochloa ruziziensis sown simultaneously (CRS to corn, and corn without intercropping (CWI. The seeds of grasses were sown in spacing of 0.34 m, being sown with a seed drill with disc coulters mounted mechanism for no-tillage system at a depth of 0.03 m. There was no significant difference between the single corn tillage and intercropping with different modalities of forage genus Panicum and Urochloa to the leaf contents of N, P, K, Ca and Mg, demonstrating the non-compete forages with corn in a intercrop on the absorption of these nutrients. In respect to S, CTS presented higher content of S foliar when compared to CWI (Table 1. The absorption of nutrients by corn are not affect by the intercrop with forages of the genus Panicum and Urochloa, in simultaneously sown.

  5. Dietary branched-chain amino acid (BCAA) and tumor growth

    Energy Technology Data Exchange (ETDEWEB)

    Chan, W.; Baron, L.; Baron, P.; White, F.; Banks, W.L. Jr.


    The effects of high dietary BCAA on tumor growth was examined in adult male Fischer 344 rats inoculated with 10/sup 6/ viable MCA fibrosarcoma cells. Ten days after tumor inoculation, when tumors were of palpable size, rats were divided into two groups at random. The experimental(E) group was fed the AIN-76 diet supplemented with 4X the BCAA content of diet casein and the control(C) group was fed the AIN-76 made isonitrogenous with the E diet by glutamic acid supplementation. Five rats from each group were killed at days 0,3,6, and 9. Rats were injected with /sup 14/C-Tyrosine and /sup 3/H-Thymidine i.p. (2 and 4 uCi/100g BW, respectively) an hour before they were killed. The incorporation of /sup 14/C and /sup 3/H into the acid insoluble fraction of the tumor tissues samples were measured. Single cell suspension of tumor were prepared for cell cycle kinetics analysis using a Coulter EPICS IV flow microflorometer. The percentage of normal and hyperdiploid cells were analyzed. Results showed that both tumor size and weight were doubled at each time point the rats were killed. At day 0, the /sup 3/H and /sup 14/C incorporation were 32 +/- 10dpm and 27 +/- 4dpm/mg tumor, respectively. The /sup 3/H incorporation dropped in both diet groups at days 6 and 9 but the /sup 14/C incorporation showed a decrease only at day 9. These changes were statistically significant, P>0.05. No difference in the tumor growth parameters used in this study can be attributed to the high dietary BCAA.

  6. Prevalence of Beta-Thalassemia in premarital screening in Al-Hassa, Saudi Arabia

    International Nuclear Information System (INIS)

    The Al-Hassa area is one of the regions in Saudi Arabia where hemoglobinopathies are prevalent. The Saudi Ministry of Health designed a protocol for premarital testing after the royal decree in December 2003. The protocol was implemented in a February 2004 order. The aim of this study was to determine the prevalence of beta-thalassemia trait among subjects coming for premarital screening in the Al-Hassa area. From February 2004 to November 2004, healthy subjects coming to six marriages consultation centers in the Al-Hassa area underwent routine mandatory tests. Subjects were considered to have beta-thalassemia trait if they had a mean corpuscular volume (MCV), 80 fL and/or a mean corpuscular hemoglobin (MCH) 3.2%. Venous blood was taken into ETDA tube and the complete blood count and red blood cell indices were measured by a Coulter automated cell counter on the same day of hemoglobin collection. Electrophoresis was done on cellulose acetate. All Saudi participants (n=8918), including 4218 (47.3%) males and 4700 (52.7%) females were screened. The prevalence of beta-thalassemia trait with high hemoglobin A2 and microcytic hypochromic anemia was 3.4% (307/8918). In countries with a high prelevance of hemoglobinopathies, a premarital screening program is helpful for identification and prevention of high-risk marriages. With a 3.4% prevalence of beta-thalassemia trait in premarital couples, future comprehensive programs are needed to know the actual prevalence of beta-thalassemia in Al-Hassa. (author)

  7. Correlation of Serum Concentrations of Soluble Thrombomodulin, Soluble Vascular Cell Adhesion Molecule-1,Intracellular Adhesion Molecule -1 And E-Selectin In Patients WithSystemic Lupus Erythematosus

    Directory of Open Access Journals (Sweden)

    Malak., A. Mohsen*, Magda.A.Gamil*,Maha. I.Shehata


    Full Text Available To date no specific serological parameters are available to assess disease activity in systemic lupus erythematosus (SLE. The objective of this study was to correlate serum levels of thrombomodulin (TM, intracellular adhesion molecule-1 sICAM-1, vascular cell adhesion molecule-1 sVCAM-1, and E-selectin with standard laboratory tests and clinical indices of disease activity in 40 patients with SLE and 20 apparently healthy persons as controls. According to British Isles Lupus Assessment Group (BILAG disease activity index, the 40 patients were divided into two groups, the first consisted of 22 with active disease, and the second consisted of 18 patients with inactive SLE. Serum sTM, sICAM-1, sVCAM-I, and E-selectin were measured in their sera, using enzyme linked immuonosorbent assay (ELISA technique.C-reactiv protein (CRP, Erythrocyte sedimentation rates (ESR and serum creatinines were measured by standard laboratory tests. Total leukocytic count and hemoglobin concentration were detected by coulter counter. Levels of sTM and sVCAM were highly elevated in the group of patients with active SLE as compared to the inactive one (P0.05. In SLE, the BILAG disease activity index, ESR and serum creatinine correlated best with sTM, sVCAM-1 and E-selectin levels while there was a weak association between CRP levels and the adhesion molecules, and no correlation between CRP level and disease activity. In conclusion, sTM and sVCAM were the most important serological indices of disease activity in SLE and might be valuable serological parameters for monitoring therapy.

  8. Infection-stimulated Anemia Results Primarily from Interferon Gamma-dependent, Signal Transducer and Activator of Transcription 1-independent Red Cell Loss

    Institute of Scientific and Technical Information of China (English)

    Zheng Wang; Dong-Xia Zhang; Qi Zhao


    Background:Although the onset of anemia during infectious disease is commonly correlated with production of inflammatory cytokines,the mechanisms by which cytokines induce anemia are poorly defined.This study focused on the mechanism research.Methods:Different types of mice were infected perorally with Toxoplasma gondii strain ME49.At the indicated times,samples from each mouse were harvested,processed,and analyzed individually.Blood samples were analyzed using a Coulter Counter and red blood cell (RBC) survival was measured by biotinylation.Levels of tumor necrosis factor-α (TNF-α),inducible nitric oxide synthase (iNOS),and inducible protein 10 (IP-10) mRNA in liver tissue were measured by real-time polymerase chain reaction.Results:T.gondii-infected mice exhibited anemia due to a decrease in both erythropoiesis and survival time of RBC in the circulation (P < 0.02).In addition,infection-stimulated anemia was associated with fecal occult,supporting previous literature that hemorrhage is a consequence of T.gondii infection in mice.Infection-induced anemia was abolished in interferon gamma (IFNγ) and IFNγ receptor deficient mice (P < 0.05) but was still evident in mice lacking TNF-α,iNOS,phagocyte NADPH oxidase or IP-10 (P < 0.02).Neither signal transducer and activator of transcription 1 (STAT1) deficient mice nor 129S6 controls exhibited decreased erythropoiesis,but rather suffered from an anemia resulting solely from increased loss of circulating RBC.Conclusions:Infection-stimulated decrease in erythropoiesis and losses of RBC have distinct mechanistic bases.These results show that during T.gondii infection,IFNγ is responsible for an anemia that results from both a decrease in erythropoiesis and a STAT1 independent loss of circulating RBC.

  9. Unique ability of the Proteus mirabilis capsule to enhance mineral growth in infectious urinary calculi. (United States)

    Dumanski, A J; Hedelin, H; Edin-Liljegren, A; Beauchemin, D; McLean, R J


    Struvite (MgNH4PO4.6H2O) calculi are a common complication of Proteus mirabilis urinary tract infections. Although urease is a major virulence factor in calculus formation, the polysaccharide capsule (CPS) of this organism also enhances struvite crystallization and growth in vitro (L. Clapham, R. J. C. McLean, J. C. Nickel, J. Downey, and J. W. Costerton, J. Crystal Growth 104:475-484, 1990). We obtained purified CPS, of known structure and varying anionic character, from P. mirabilis ATCC 49565 and several other organisms. Artificial urine was added to CPS, and the pH was elevated from 5.8 to 8.5 by the addition of urease or titration with 0.25 M NH4OH to induce struvite crystallization. Crystallization was measured by particle counting (Coulter counter), and the morphology (crystal habit) was examined by phase-contrast microscopy. In the presence of partially anionic P. mirabilis CPS, struvite formation occurred at a lower pH than in the absence of CPS or in the presence of other neutral, partially anionic, or anionic CPS. At pH 7.5 to 8.0, significantly more struvite crystals formed in the presence of P. mirabilis CPS than under other experimental conditions. With the exception of one polymer (curdlan) which did not bind Mg2+, enhancement of struvite formation by CPS polymers was inversely proportional to their Mg2+ binding ability. We speculate that the structure and partial anionic nature of P. mirabilis CPS enable it to enhance struvite formation by weakly concentrating Mg2+ ions during struvite crystal formation. This illustrates a new virulence aspect of bacterial CPS during infection. PMID:8005688


    Directory of Open Access Journals (Sweden)

    Mustafa AKIL


    Full Text Available The objective of this study is to analyze the effects of acute exercises the sedentary individuals do on thyroid hormonal metabolism. The research was carried out on 14 sedentary males between the ages of 18 and 24. The study was approved by the local committee for ethics. The average age of the subjects was: 20,4 ± 0,65 years, their average body weights: 76,86 ± 2,08 kg and their average height was: 175,07 ± 1,26 cm. As for the exercise application, the subjects were made tired through Bruce protocol.10 cc of blood samples were taken from the subjects each time before the exercise, after the exercise, 24 hours after the exercise and 48 hours after the exercise. In the obtained blood samples, the parameters of serum TSH, T3, T4, were analyzed in Backman LX-20 auto analyzer through Lot No:T1142, Mg Lot No:907035, P Lot No:903417 Backman Coulter test kits. The arithmetic averages, and standard deviations of all the parameters were determined. The repeating ANOVA test was used for the determination of the difference between the parameters. Confidence interval was chosen as 95% and the values of p≤0.05 and below was accepted as statistically significant. According to the measurements before and after the exercises, significant differences were found between the parameters of TSH, T3, and T4 (p≤0.05.In conclusion, it can be said that the protocol of these acute submaximal exercises done by sedentary individuals decreased serum TSH, T3, and T4 levels and it approached to normal levels 48 hours after the exercises.

  11. Radioprotection conferred by dextran sulfate given before irradiation in mice

    International Nuclear Information System (INIS)

    Dextran sulfate (DS) has been observed to cause mobilization (fivefold) of hemopoietic stem cells (HSC) and leukocytes, primarily lymphocytes, into the peripheral blood of mice within 2-3 h after intraperitoneal (i.p.) injection. This effect was dose dependent and was prolonged for several hours when the high-molecular-weight version DS500 (500,000 daltons) was used. When DS500 was given 1-3 days before irradiation, hemopoietic recovery was markedly enhanced. Postirradiation injection was ineffective. By ten days after irradiation (7.0 Gy), the number of endogenous spleen colonies (CFUs) and the splenic mass were much larger if DS pretreatment had been given. This effect was dependent on the dose of DS500 and on the time administered, 60 mg/kg producing a maximal effect when given three days before irradiation. DS500 caused a transient anaphylactoid shock, however, in most mice--mild at low doses but potentially lethal at doses above 40 mg/kg (10% mortality within 1-3 days after 60 mg/kg). The following results were obtained with 50 mg/kg, a compromise dose causing minimal mortality (3%) given three days before irradiation. Reticulocyte reappearance was earlier in irradiated mice given DS500, indicating earlier erythropoietic recovery. Some of these reticulocytes were resistant to lysing agents, so their appearance could be detected using the Coulter electronic cell counter, as well as in stained blood smears. The 30-day mortality due to bone marrow failure after irradiation was significantly decreased in DS-treated mice below 9.5 Gy, and the LD50/30 was increased by 0.5 Gy. This study shows that dextran sulfate exerts a radioprotective influence on the hemopoietic system and hence survival when administered prophylactically

  12. Nanopore Analysis of Nucleic Acids: Single-Molecule Studies of Molecular Dynamics, Structure, and Base Sequence (United States)

    Olasagasti, Felix; Deamer, David W.

    Nucleic acids are linear polynucleotides in which each base is covalently linked to a pentose sugar and a phosphate group carrying a negative charge. If a pore having roughly the crosssectional diameter of a single-stranded nucleic acid is embedded in a thin membrane and a voltage of 100 mV or more is applied, individual nucleic acids in solution can be captured by the electrical field in the pore and translocated through by single-molecule electrophoresis. The dimensions of the pore cannot accommodate anything larger than a single strand, so each base in the molecule passes through the pore in strict linear sequence. The nucleic acid strand occupies a large fraction of the pore's volume during translocation and therefore produces a transient blockade of the ionic current created by the applied voltage. If it could be demonstrated that each nucleotide in the polymer produced a characteristic modulation of the ionic current during its passage through the nanopore, the sequence of current modulations would reflect the sequence of bases in the polymer. According to this basic concept, nanopores are analogous to a Coulter counter that detects nanoscopic molecules rather than microscopic [1,2]. However, the advantage of nanopores is that individual macromolecules can be characterized because different chemical and physical properties affect their passage through the pore. Because macromolecules can be captured in the pore as well as translocated, the nanopore can be used to detect individual functional complexes that form between a nucleic acid and an enzyme. No other technique has this capability.

  13. Spatial and temporal variability in aggregated grain-size distributions, with implications for sediment dynamics (United States)

    Wheatcroft, Robert A.; Butman, Cheryl Ann


    The grain-size distribution of bottom sediments has important implications for diverse aspects of sediment dynamics, including prediction of the critical boundary shear stress and calibration of suspended sediment sensors. Past sampling strategies to obtain estimates of the seabed grain-size distribution typically have not considered spatial and temporal variability, and have been insufficient to resolve potential millimeter-scale vertical variations in grain size. Moreover, laboratory analyses have been predicated on chemically and/or ultrasonically disaggregating the sediments before resolving particle diameter, therefore the more dynamically relevant in situ grain-size spectrum is not measured. To test for such effects, three sites on the northern California continental shelf comprising a cross-shelf transect from a sandy, inner-shelf (60 m) site, to a muddy, mid-shelf (90 m) site and a relict, outer-shelf (130 m) site were studied. Replicate box cores were collected over two winter field seasons, and multiple subcores from each box core were vertically sectioned at 2-mm intervals. Gentle wet sieving techniques were used to determine the mass fraction in the 300,um size classes. In addition, a lesser number of standard disaggregated grain-size analyses were performed using a Coulter Counter. Results from the sandy, inner-shelf site indicate the presence of an ephemeral fine-grained (flow. In addition, there is evidence for a progressive and substantial winnowing of fine-grained sediment from the surface layer over the course of a winter storm season. At the deeper sites, the upper 2 mm of the bed contained 5-20% more material meters) scale spatial variability is modest. In addition, the disaggregated grain-size distribution at the two muddy sites is, in all cases, markedly finer than the in situ grain-size distribution. Therefore, calibrations and predictions based on a knowledge of the size distribution of the primary (i.e. disaggregated) particles could be in

  14. Feasibility of using a particle counter or flow-cytometer for bacterial enumeration in the assimilable organic carbon (AOC) analysis method. (United States)

    Aggarwal, Srijan; Jeon, Youchul; Hozalski, Raymond M


    Assimilable organic carbon (AOC) is one of the major determinants of microbial growth and stability in drinking water distribution systems. Nevertheless, AOC measurements are rarely conducted in practice owing, in part, to the tedious and time-consuming nature of the bioassay. Herein, we compared three alternative cell count approaches [flow cytometry with staining (FC-S), flow cytometry without staining (FC-NS), and particle counting (Coulter counter; CC)] for bacterial enumeration as a means to expedite the AOC bioassay. Our results suggest that of the three methods only FC-S provides a suitable alternative to plate counting for rapid and accurate enumeration of both P17 and NOX in the AOC bioassay. While the cell counts obtained by FC-NS were linearly correlated with those obtained using the traditional heterotrophic plate count (HPC) method (FC-NS: R(2) = 0.89-0.96), the AOC values obtained by FC-NS were overestimated by 18-57 %. The CC approach was unsuccessful in enumerating Spirillum strain NOX cells because of the relatively small size of that organism. The CC counts were linearly correlated with HPC for Pseudomonas fluorescens strain P-17 (P17) cells (R(2) = 0.83) but like FC-NS, the CC approach also overestimated the AOC values (for P-17). The advantage of the FC-S method over the other two is improved sensitivity and the ability to specifically enumerate whole cells (and likely viable) as opposed to non-viable cells, cell debris, and other contaminating particles introduced by the test water itself or sample handling. PMID:26139595

  15. Formation of microscopic particles during the degradation of different polymers. (United States)

    Lambert, Scott; Wagner, Martin


    This study investigated the formation and size distribution of microscopic plastic particles during the degradation of different plastic materials. Particle number concentrations in the size range 30 nm-60 μm were measured by nanoparticle tracking analysis (NTA) and Coulter Counter techniques. Each of the plastics used exhibited a measureable increase in the release of particles into the surrounding solution, with polystyrene (PS) and polylactic acid (PLA) generating the highest particle concentrations. After 112 d, particle concentrations ranged from 2147 particles ml(-1) in the control (C) to 92,465 particles ml(-1) for PS in the 2-60 μm size class; 1.2 × 10(5) particles ml(-1) (C) to 11.6 × 10(6) for PLA in the 0.6-18 μm size class; and 0.2 × 10(8) particles ml(-1) (C) to 6.4 × 10(8) particles ml(-1) for PS in the 30-2000 nm size class (84 d). A classification of samples based on principal component analysis showed a separation between the different plastic types, with PLA clustering individually in each of the three size classes. In addition, particle size distribution models were used to examine more closely the size distribution data generated by NTA. Overall, the results indicate that at the beginning of plastic weathering processes chain scission at the polymer surface causes many very small particles to be released into the surrounding solution and those concentrations may vary between plastic types. PMID:27470943

  16. Primum Non Nocere: is shared decision-making the answer? (United States)

    Santhirapala, Ramai; Moonesinghe, Ramani


    Surgical ambition is rising, with the Royal College of Surgeons reporting an increase in the number of procedures by a million over the past decade (Royal College of Surgeons. Surgery and the NHS in Numbers. Available from Underpinning, this is a rapidly growing population, especially those in the over 85 age group, coupled with rising perioperative expertise; options for surgery are now present where conditions were once managed conservatively. Matching the right patient to the right procedure has never been so pertinent (Bader, Am Soc Anesthesiol 78(6), 2014). At the heart of these increasingly complex decisions, which may prove fatal or result in serious morbidity, lies the aspiration of shared decision-making (SDM) (Glance et al., N Engl J Med 370:1379-81, 2014). Shared decision-making is a patient-centred approach taking into account the beliefs, preferences and views of the patient as an expert in what is right for them, supported by clinicians who are the experts in diagnostics and valid therapeutic options (Coulter and Collins, Making shared decision-making a reality: no decision about me, without me, 2011). It has been described as the pinnacle of patient-centred care (Barry et al., N Engl J Med 366:780-1, 2012). In this commentary, we explore further the concept of shared decision-making, supported by a recent article which highlights critical deficits in current perioperative practice (Ankuda et al., Patient Educ Couns 94(3):328-33, 2014). This article was chosen for the purposes of this commentary as it is a large study across several surgical specialties investigating preoperative shared decision-making, and to our knowledge, the only of this kind. PMID:27313845

  17. Anemia em escolares da primeira série do ensino fundamental da rede pública de Maceió, Alagoas, Brasil

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    Célia Dias dos Santos


    Full Text Available Realizou-se um estudo transversal com amostra probabilística de 426 alunos de 6 a 10 anos, da 1ª série do ensino fundamental das escolas públicas de Maceió, Alagoas, Brasil. O objetivo foi avaliar a prevalência da anemia e sua associação com o retardo de crescimento. Os dados foram coletados entre maio e julho de 2000. O sangue foi colhido por venipuntura e a dosagem de hemoglobina (Hb foi realizada pelo contador Coulter STKS. Classificou-se a anemia por dois critérios estabelecidos pela Organização Mundial da Saúde: Hb <11,5g/dl e Hb <12,0g/dl. O retardo pondo-estatural foi diagnosticado quando os indicadores Altura/Idade (A/I, Peso/Idade (P/I e Peso/Altura (P/A se encontravam abaixo de -2,0 desvios-padrão da referência NCHS. A prevalência da anemia foi de 9,9%, considerando-se Hb <11,5g/dl e de 25,4%, utilizando-se Hb <12,0g/dl. O retardo do crescimento foi detectado em 6,2% segundo A/I, 4,0% pelo indicador P/I e 3,0% em relação a P/A. Não houve associação estatisticamente significativa entre as variáveis do estudo. Estes resultados confirmam os observados em outras pesquisas: prevalência de anemia muito superior à prevalência do retardo pondo-estatural. Conclui-se que as graves conseqüências da anemia nesta faixa etária, justificam a implementação de políticas de largo alcance para o enfrentamento do problema.

  18. Identifying the AD 1257 Salamas volcanic event from micron-size tephra composition in two East Antarctic ice cores (United States)

    Petit, Jean Robert; Narcisi, Biancamaria; Batanova, Valentina G.; Joël, Savarino; Komorowski, Jean Christophe; Michel, Agnes; Metrich, Nicole; Besson, Pascale; Vidal, Celine; Sobolev, Alexander V.


    A wealth of valuable data about the history of explosive volcanic history can be extracted from polar ice successions. Both the volatile by-products and the solid silicate (tephra) components of volcanic plumes can be incorporated into snow layers, providing tools for chronostratigraphic correlations and for interpretation of climate-volcanism interactions. Volcanic events from low-latitude regions are of particular interest as the related sulphate aerosol travelling through the stratosphere can reach the polar sheets forming inter-hemispheric (Greenland and Antarctica) signals preserved in the ice. Within the glaciological record of globally significant volcanic markers, the AD1259 signal represents one of most prominent events over the last thousands years. Its source has been long debated. On the basis of recent field investigations (Lavigne et al., 2013; Vidal et al., 2015), it has been proposed that Mount Samalas on Lombok Island (Indonesia) represents the source responsible for the polar event. With the goal of bringing distal tephrochronological evidence to source identification, we have attempted to identify volcanic ash associated to the AD 1259 sulphate pulse. To this purpose we used firn and ice-core samples from two East Antarctic Plateau sites: Concordia-Dome C (75°06' S, 123°20' E, 3233 m) and Talos Dome (72°49'S, 159°11'E, 2315 m). Our high-resolution studies included sample processing in a Class 100 clean room using established ultra-clean procedures for insoluble microparticle analyses, Coulter counter grain size measurements, scanning electron microscope observations and the geochemical (major elements) composition from the recently set ISTERRE Jeol JXA 8230 Superprobe and calibrated for small particles analysis. Despite the difficulty of studying such minute fragments, within both cores we located and characterised multiple tiny (micron-size) glass shards concomitant with the volcanic peak. We present preliminary results alongside comparison

  19. Aquaporin expression and cell volume regulation in the SV40 immortalized rat submandibular acinar cell line. (United States)

    Hansen, Ann-Kristin; Galtung, Hilde Kanli


    The amount of aquaporins present and the cellular ability to perform regulatory volume changes are likely to be important for fluid secretions from exocrine glands. In this work these phenomena were studied in an SV40 immortalized rat submandibular acinar cell line. The regulatory cell volume characteristics have not previously been determined in these cells. Cell volume regulation following hyposmotic exposure and aquaporin induction was examined with Coulter counter methodology, radioactive efflux studies, fura-2 fluorescence, and polymerase chain reaction and Western blot techniques. Cell volume regulation was inhibited by the K(+) channel antagonists quinine and BaCl(2) and the Cl(-) channel blocker 5-nitro-2-(3-phenypropylamino)benzoic acid. A concomitant increase in cellular (3)H-taurine release and Ca(2+) concentration was also observed. Chelation of both intra- and extracellular Ca(2+) with EGTA and the Ca(2+) ionophore A23187 did not, however, affect cell volume regulation. Aquaporin 5 (AQP5) mRNA and protein levels were upregulated in hyperosmotic conditions and downregulated upon return to isosmotic solutions, but were reduced by the mitogen-activated ERK-activating kinase (MEK) inhibitor U0126. A 24-h MEK inhibition also diminished hyposmotically induced cell swelling and cell volume regulation. In conclusion, it was determined that regulatory volume changes in this immortalized cell line are due to KCl and taurine efflux. In conditions that increased AQP5 levels, the cells showed a faster cell swelling and a more complete volume recovery following hyposmotic exposure. This response could be overturned by MEK inhibition. PMID:17021794

  20. Quantificação de células CD 34+ em sangue periférico, produto de aférese e cordão umbilical: estudo comparativo de três diferentes metodologias

    Directory of Open Access Journals (Sweden)

    Bacal Nydia S.


    Full Text Available A quantificação das células CD34+ em sangue periférico é utilizada para determinar o melhor momento de iniciar a aférese, enquanto que na leucoaférese e no sangue de cordão umbilical determinam a quantidade de células CD34+ para o transplante de células progenitoras. O objetivo deste trabalho foi comparar três diferentes metodologias de quantificação de células CD34+. Foram utilizados três diferentes tipos de amostras: a 32 amostras de sangue periférico, coletadas de pacientes estimulados com G-CSF 50 mg/Kg/dose total, sem quimioterapia na mobilização. b 31 amostras de produto de aférese de pacientes estimulados com o mesmo protocolo de G-CSF. c 20 amostras de sangue de cordão coletadas em CPDA-1, por punção de veia umbilical. As amostras permaneceram à temperatura ambiente no máximo até 24h da análise. O citômetro de fluxo utilizado foi o Epics XL-MCL (Coulter com os protocolos de dupla plataforma: ISHAGE e Mulhouse modificado para análise de maior numero de eventos, e o citômetro Imagn 2000 (Biometric Imaging de plataforma única, conforme técnicas recomendadas. Os anticorpos monoclonais utilizados foram: CD45-FITC, CD34-PE, e isotipo IgG1-PE da Immunotech. As análises estatísticas foram: ANOVA e correlação de teste t de Student. Os resultados não apresentaram diferenças estatisticamente significativas nos três métodos.

  1. Classification of biological cells using a sound wave based flow cytometer (United States)

    Strohm, Eric M.; Gnyawali, Vaskar; Van De Vondervoort, Mia; Daghighi, Yasaman; Tsai, Scott S. H.; Kolios, Michael C.


    A flow cytometer that uses sound waves to determine the size of biological cells is presented. In this system, a microfluidic device made of polydimethylsiloxane (PDMS) was developed to hydrodynamically flow focus cells in a single file through a target area. Integrated into the microfluidic device was an ultrasound transducer with a 375 MHz center frequency, aligned opposite the transducer was a pulsed 532 nm laser focused into the device by a 10x objective. Each passing cell was insonfied with a high frequency ultrasound pulse, and irradiated with the laser. The resulting ultrasound and photoacoustic waves from each cell were analyzed using signal processing methods, where features in the power spectra were compared to theoretical models to calculate the cell size. Two cell lines with different size distributions were used to test the system: acute myeloid leukemia cells (AML) and melanoma cells. Over 200 cells were measured using this system. The average calculated diameter of the AML cells was 10.4 +/- 2.5 μm using ultrasound, and 11.4 +/- 2.3 μm using photoacoustics. The average diameter of the melanoma cells was 16.2 +/- 2.9 μm using ultrasound, and 18.9 +/- 3.5 μm using photoacoustics. The cell sizes calculated using ultrasound and photoacoustic methods agreed with measurements using a Coulter Counter, where the AML cells were 9.8 +/- 1.8 μm and the melanoma cells were 16.0 +/- 2.5 μm. These results demonstrate a high speed method of assessing cell size using sound waves, which is an alternative method to traditional flow cytometry techniques.

  2. Cell diameter measurements obtained with a handheld cell counter could be used as a surrogate marker of G2/M arrest and apoptosis in colon cancer cell lines exposed to SN-38

    International Nuclear Information System (INIS)

    Highlights: •Chemo-sensitivity to SN-38 was assayed by the automated cell counter. •Colon cancer cell line, HCT116 cells were more sensitive to SN-38 than HT29 cells. •Increase of cell size reflects G2/M arrest. •Appearance of small particles indicates cell apoptosis. -- Abstract: In vitro assessment of chemosensitivity are important for experiments evaluating cancer therapies. The Scepter 2.0 cell counter, an automated handheld device based on the Coulter principle of impedance-based particle detection, enables the accurate discrimination of cell populations according to cell size and volume. In this study, the effects of SN-38, the active metabolite of irinotecan, on the colon cancer cell lines HCT116 and HT29 were evaluated using this device. The cell count data obtained with the Scepter counter were compared with those obtained with the 3H-thymidine uptake assay, which has been used to measure cell proliferation in many previous studies. In addition, we examined whether the changes in the size distributions of these cells reflected alterations in the frequency of cell cycle arrest and/or apoptosis induced by SN-38 treatment. In our experiments using the Scepter 2.0 cell counter, the cell counts were demonstrated to be accurate and reproducible measure and alterations of cell diameter reflected G2/M cell cycle arrest and apoptosis. Our data show that easy-to-use cell counting tools can be utilized to evaluate the cell-killing effects of novel treatments on cancer cells in vitro

  3. Comparison of cell counting methods in rodent pulmonary toxicity studies: automated and manual protocols and considerations for experimental design. (United States)

    Zeidler-Erdely, Patti C; Antonini, James M; Meighan, Terence G; Young, Shih-Houng; Eye, Tracy J; Hammer, Mary Ann; Erdely, Aaron


    Pulmonary toxicity studies often use bronchoalveolar lavage (BAL) to investigate potential adverse lung responses to a particulate exposure. The BAL cellular fraction is counted, using automated (i.e. Coulter Counter®), flow cytometry or manual (i.e. hemocytometer) methods, to determine inflammatory cell influx. The goal of the study was to compare the different counting methods to determine which is optimal for examining BAL cell influx after exposure by inhalation or intratracheal instillation (ITI) to different particles with varying inherent pulmonary toxicities in both rat and mouse models. General findings indicate that total BAL cell counts using the automated and manual methods tended to agree after inhalation or ITI exposure to particle samples that are relatively nontoxic or at later time points after exposure to a pneumotoxic particle when the response resolves. However, when the initial lung inflammation and cytotoxicity was high after exposure to a pneumotoxic particle, significant differences were observed when comparing cell counts from the automated, flow cytometry and manual methods. When using total BAL cell count for differential calculations from the automated method, depending on the cell diameter size range cutoff, the data suggest that the number of lung polymorphonuclear leukocytes (PMN) varies. Importantly, the automated counts, regardless of the size cutoff, still indicated a greater number of total lung PMN when compared with the manual method, which agreed more closely with flow cytometry. The results suggest that either the manual method or flow cytometry would be better suited for BAL studies where cytotoxicity is an unknown variable. PMID:27251196

  4. Prevalence of Anemia and Associated Factors in Child Bearing Age Women in Riyadh, Saudi Arabia

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    AlJohara M. AlQuaiz


    Full Text Available Objective. To determine the prevalence and risk factors for anemia in child bearing age women in Riyadh, Saudi Arabia. Design. Cross-sectional survey was conducted using two-stage cluster sampling. 25 clusters (primary health care centers (PHCC were identified from all over Riyadh, and 45–50 households were randomly selected from each cluster. Eligible women were invited to PHCC for questionnaire filling, anthropometric measurements, and complete blood count. Blood hemoglobin was measured with Coulter Cellular Analysis System using light scatter method. Setting. PHCC. Subjects. 969 (68% women out of 1429 women were included in the analysis. Results. Mean hemoglobin was 12.35 (±1.80 g/dL, 95% CI 12.24–12.46 with interquartile range of 1.9. Anemia (Hb <12 g/dL was present in 40% (390 women. Mean (±SD for MCH, MCV, MCHC, and RDW was 79.21 (±12.17 fL, 26.37 (±6.21 pg, 32.36 (±4.91 g/dL, and 14.84 (±4.65%, respectively. Multivariate logistic regression revealed that having family history of iron deficiency anemia (OR 2.91, 95% CI 1.78–4.76 and infrequent intake of meat (OR 1.54, 95%CI 1.15–2.05 were associated with increased risk of anemia, whereas increasing body mass index (OR 0.95, 95% CI 0.92–0.97 was associated with reduced risk of anemia. Conclusion. Women should be educated about proper diet and reproductive issues in order to reduce the prevalence of anemia in Saudi Arabia.

  5. Grazing and metabolism of Euphausia pacifica in the Yellow Sea.

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    Zhencheng Tao

    Full Text Available Grazing and metabolism of Euphausia pacifica in the Yellow Sea were studied from September 2006 to August 2007. Euphausia pacifica is a selective-feeding omnivore and grazing rates among different months were monitored using a Coulter Counter and batch culture feeding experiments. Euphausia pacifica mainly grazed microzooplankton in August and September, which resulted in an increase in chlorophyll a concentration. Oxygen consumption rate of E. pacifica was 38.7-42.5 μmol O2 g(-1 DW h(-1 in March, which was four times higher than the oxygen consumption rates in September and December. The vigorous metabolism of E. pacifica in March consumed 3.1% of body carbon daily, which is likely related to its high reproduction and grazing rate. Respiration and metabolism of E. pacifica in September and December were similar and were lower. O:N ratio of E. pacifica was the highest (17.3-23.8 in March when spawning activity occurred and when food was abundant. The energetic source of E. pacifica during September and December was mostly protein from eating a carnivorous diet, including such items as microzooplankton. Euphausia pacifica was found in cold water at the bottom of the Yellow Sea in summer and autumn and maintained a low consumption status. O:N ratios of E. pacifica in March, September, and December were negatively correlated with SSTs and no significant correlation was found between O:N ratios and chlorophyll a concentration. Seawater temperature is clearly the most important parameter influencing the metabolism of E. pacifica.

  6. 蓝色鳞(Cyprinus carpio blue vat)的细胞遗传学分析%Cytogenetic Analysis of Blue Common Carp Cyprinus carpio blue var

    Institute of Scientific and Technical Information of China (English)

    薛淑群; 徐伟


    The diploid chromosome number, karyotype, and DNA content were studied in metaphase chromosome in kidney cells and blood of blue common carp Cyprinus carpio blue var injected with PHA and colchicin by airdrying technique. It was found that the diploid chromosome number was 2n=100. The karyotype was found to be composed of 30 m, 26sm and 44st+t chromosomes, and NF=156. The diploid nucleus DNA content was measured from the somatic cell of the blue common carp using flow cytometer (Beckman cellLab Quant, made in USA) and the DNA content (2.3 pg· N^+) in erythrocytes of chick (Gallus sp) as standard. The common carp had DNA content of3.99pg·N^-1, corresponding to the result measured chromosome number as ploidy.%采用体内注射PHA和秋水仙素,肾细胞短期培养,常规空气干燥法制备蓝色鳞鲤染色体,对100个中期分裂相记数统计,确定蓝色鳞鲤(Cyprinus carpio blue var)的染色体数为2n=100。测得核型参数按Levan等的染色体划分标准得出:蓝色鳞鲤有15对中部着丝点染色体(m);13对亚中部着丝点染色体(sin);22对端部和亚端部着丝点染色体(st,t),其染色体总臂数(NF)为156,蓝色鳞鲤核型公式为2n=30m+26sm+44st,t。采用流式细胞分析仪测定了蓝色鳞鲤的DNA含量,与鸡血细

  7. White blood cells of peripheral blood with ConA-positive glycotopes in patients with chronic leukemia

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    G. S. Maslak


     Tumor growth progression of blood cells occurs due to changes in their genetic apparatus, which affects not only the cells morphological characteristics, but also their functional activity which to a greater extent depends on the membrane surface structures, a significant part of which is of glycoprotein nature. Complex type N-glycans are components of surface glycoproteins in the most of leukocytes. Thus, the study of changes in carbohydrate determinants of glycoproteins on the surface of leucocytes in tumorigenesis can help to reveal the mechanisms of this process. The aim of our study was to investigate the monocytes and granulocytes cytoplasmic membrane N-glycosylation in patients with chronic leukemia. The object of the study were blood cells of patients with chronic lymphocytic leukemia (n = 12 and polycythemia vera (n = 15 aged 58–66 years. Healthy hematologic volunteers (n = 15 aged 55 to 65 years were in the control group. N-glycan exposure on monocytes and granulocytes was investigated by flow cytometer Beckman Сoulter EPICS with Canavalia ensiformis lectin – Con A conjugated with fluorescent labels. The number of dead cells was monitored by means of binding them with propidium iodide. The result has been analyzed with FC Express. According to our data, levels of ConA-positive monocytes and granulocytes were 9,9 ± 1,0% and 32,7 ± 3,2%, respectively, in peripheral blood of healthy persons. The level of ConA-positive monocytes decreased to 31,0 ± 2,3% and the number of ConA-binding granulocytes increased to 66,7 ± 3,8% in patients with chronic lymphocytic leukemia compared with the norm. The number of ConA-positive monocytes decreased 3.3 times, and the level of granulocytes interacting with Canavalia ensiformis lectin slightly increased relative to control in polycythemia vera patients. There is significant increase in Con A-positive epitopes on granulocytes in patients with chronic lymphocytic leukemia and polycythemia vera compared with the

  8. Content Of 2,4-D-14C Herbicide Residue In Water And Soil Of Irrigated Rice Field System

    International Nuclear Information System (INIS)

    The investigation of 2,4-D exp.-14C herbicide residue in water and soil of irrigated rice field system was carried out. Rice plant and weeds (Monochoria vaginalis Burn. F. Presl) were planted in 101 buckets using two kinds of soil condition, I.e. normal soil and 30 % above normal compact soil. After one week planting, the plants were sprayed with 1 u Ci of 2,4-D exp.-14C and 0,4 mg non labeled 2,4-D. The herbicide residue content was determined 0, 2, 4, 8 and 10 weeks after spraying with 2,4-D herbicide. The analysis was done using Combustion Biological Oxidizer merk Harvey ox-400, and counted with Liquid Scintillation Counter merk Beckman model LS-1801. The results indicates that the herbicide contents in water and soil decrease from the first spraying with herbicide until harvest herbicide Residue content in water after harvest was 0.87 x 10 exp.-6 ppm for soil normal condition, and 0.59 x 10 exp.-6 pm for the soil 30 % up normal condition, while herbicide content in soil was 1.54 x 10 exp.-6 ppm for soil normal condition and 1.48 x 10 exp.-6 ppm for soil 30 % up normal. 2,4-D herbicide residue content in rice after harvest was 0.27 x 10 exp.-6 ppm for normal soil condition and 0.25 x 10 exp.-6 ppm for the soil 30 % up normal. 2,4-D herbicide residue content in roots and leaves of weeds after harvest were respectively 0.29 x 10 exp.-6 ppm and 0.18 x 10 exp.-6 for normal soil condition, while for 30 % up normal soil were 0.25 x 10 exp.-5 ppm and 0.63 x 10 exp.-7 ppm. This result indicates that there is no effect pollution to surrounding area, because the herbicide content is still bellow the allowed detection limit, 0.05 ppm

  9. Validation of Modified Estimated Glomerular Filtration Rate in Chronic Kidney Disease Patients

    International Nuclear Information System (INIS)

    Objective: To derive the ethnic factor and validate the modified estimated Glomerular Filtration Rate (eGFR) by Modification of Diet in Renal Disease (MDRD) equation for Chronic Kidney Disease (CKD) patients of Rawalpindi. Study Design: Cross- sectional study. Place and Duration of Study: Armed Forces Institute of Pathology (AFIP), Rawalpindi, from July 2011 to July 2012. Methodology: A total of 140 patients with CKD reporting to AFIP for GFR measurement by 99mTechnetium diethylenethiaminepenta-acetic acid (99mTc-DTPA) renal scan were consecutively inducted. Serum creatinine was measured by the Jaffe's assay on Beckman DxC 600 Analyzer prior to the renal scan. Ethnic factor for population of Rawalpindi with CKD was derived for the MDRD eGFR equation using 99mTc-DTPA renal scan by Gates method as the reference method. MDRD equation was modified by inclusion of the ethnic factor in it. Agreement between the reference GFR (rGFR) and the modified MDRD eGFR (mGFR) was assessed by applying paired samples t-test. Results: Out of 140 patients of CKD, 99 (71%) were males and 41 (29%) females, with mean age of 55 A+- 13.42 years. The mean values were 32.91 A+- 14.96, 34.89 A+- 16.45, 0.971 A+- 0.20 and 33.87 A+- 15.97 for rGFR, original eGFR, ethnic factor and mGFR respectively. The mGFR with new ethnic factor of 0.971 showed improved performance as compared to original eGFR and showed a significant level of correlation with rGFR (r2 = 0.817), at a p-value of 0.000. Conclusion: This study validates the mGFR equation by inclusion of newly derived ethnic factor of 0.971 in the population of Rawalpindi with CKD and it was found to be not significantly different from the rGFR. (author)

  10. Antigen Expression on Blast Cells and Hematological Parameters at Presentation in Acute Lymphoblastic Leukemia Patients

    International Nuclear Information System (INIS)

    Objective: To analyze the expression of various antigens on the leukemic blasts and to determine the hematological parameters, in Acute Lymphoblastic Leukemia (ALL) patients at presentation. Study Design: Observational study. Place and Duration of Study: King Edward Medical University, Lahore and Hameed Latif Hospital, Lahore, from February 2013 to March 2014. Methodology: A total of 50 newly diagnosed and untreated patients of ALL were selected from Mayo Hospital and Hameed Latif Hospital. These patients included both genders and all age groups. Hemoglobin, total leukocyte count and platelet count were determined on hematology analyser-Sysmex-Kx-2I. Blast cell percentage was estimated on Giemsa stained blood smears. Immuno phenotyping was done on bone marrow samples by 5 colour flow cytometery on Beckman Counter Navious Flow cytometer. An acute leukemia panel of 23 antibodies was used. The data was entered and analyzed in SPSS version 22. Results: Of the 50 ALL patients, 36 (72 percentage) were B-ALL and 14 (28 percentage) T-ALL. There were 18 (36 percentage) children and 32 (64 percentage) adults. T-ALL included 22 percentage of the childhood and 31 percentage of the adult cases. Immuno phenotypic analysis showed that CD19, CD79a and CD20 were B-lineage specific markers whereas cCD3, CD3 and CD5 were T-lineage specific. CD10 was the most sensitive marker for B-ALL and CD7 was the most sensitive marker of T-ALL. TdT was expressed in 92 percentage B-ALL and 71 percentage T-ALL cases, CD34 in 58 percentage and 43 percentage cases and CD45 in 83 percentage and 100 percentage respectively. High leukocyte count (> 50 x 109/L) was present in 58 percentage cases. Hemoglobin was < 10 g/dl in 74 percentage patients and platelet count was below 20 x 109/Lin 12 percentage patients. Leukocyte count, hemoglobin, platelet count and blast cell percentage did not show a significant difference in the two ALL immuno types. Conclusion: The frequency of T-ALL is higher in childhood

  11. The ability of streptomycin-loaded chitosan-coated magnetic nanocomposites to possess antimicrobial and antituberculosis activities

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    El Zowalaty ME


    Full Text Available Mohamed Ezzat El Zowalaty,1,2 Samer Hassan Hussein Al Ali,3,4 Mohamed I Husseiny,2,5 Benjamin M Geilich,6,7 Thomas J Webster,7,8 Mohd Zobir Hussein9 1Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia; 2Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt; 3Laboratory of Molecular Biomedicine, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia; 4Faculty of Pharmacy, Isra University, Amman, Jordan; 5Beckman Research Institute of City of Hope, Duarte, CA, USA; 6Department of Bioengineering, 7Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 8Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; 9Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology; Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia Abstract: Magnetic nanoparticles (MNPs were synthesized by the coprecipitation of Fe2+ and Fe3+ iron salts in alkali media. MNPs were coated by chitosan (CS to produce CS-MNPs. Streptomycin (Strep was loaded onto the surface of CS-MNPs to form a Strep-CS-MNP nanocomposite. MNPs, CS-MNPs, and the nanocomposites were subsequently characterized using X-ray diffraction and were evaluated for their antibacterial activity. The antimicrobial activity of the as-synthesized nanoparticles was evaluated using different Gram-positive and Gram-negative bacteria, as well as Mycobacterium tuberculosis. For the first time, it was found that the nanoparticles showed antimicrobial activities against the tested microorganisms (albeit with a more pronounced effect against Gram-negative than Gram-positive bacteria, and thus, should be further studied as a novel nano-antibiotic for numerous antimicrobial and antituberculosis applications. Moreover, since these nanoparticle bacteria fighters are magnetic, one can easily

  12. Comparison of the argon tunable dye laser with the flashlamp pulsed dye laser in treatment of facial telangiectasia (United States)

    Broska, Pamela; Martinho, Elena; Goodman, Matthew M.


    A prospective, side-by-side comparison study of two different lasers for the treatment of solar- induced telangiectasia was carried out in 14 patients at the Beckman Laser Institute and Medical Clinic. The argon tunable dye laser (Coherent, Palo Alto, Calif.) was used in the method modified from Orenstein and Nelson to completely treat discrete telangiectasias on one cheek. Specifically, the argon tunable dye laser (ATDL) was set at 0.7 - 0.8 watts, 585 nm wavelength, shutter-pulsed at 0.1 second duration with a spot size of 0.1 mm, and individual vessels were 'traced out' with 4X loupe magnification. Each patient's opposite cheek was then treated in the standard fashion with the flashlamp pulsed dye laser (Candela, Natick, Mass.) using a technique similar to Polla's et al. Specifically, the flashlamp pulsed dye laser (FPDL) was set at 585 nm wavelength, pulsed mode of 450 microseconds pulse duration, spot size of 5 mm, overlapping 10 - 20%, with power densities of 5.5 to 6.5 joules/cm2. All patients had symmetrical cheek telangiectasias of several years' duration. Patients were treated on day 0, and examined on weeks 2, 4, and 6. Photos were taken at each visit, and evaluation was done by questionnaire and direct observation, as well as by photographic slides later projected to an impartial panel. Final evaluation by the panel at week 6 showed 11/14 patients with excellent results (75 - 100% clearing) at sites treated with the FPDL, compared with 4/14 with the ATDL. In contrast, 4/14 FPDL sites were graded as fair to minimal improvement, and 9/14 as fair for the ATDL. The patients' self-evaluations graded the final results very similar to that of the panel. Most patients were bothered by the ecchymosis and hyperpigmentation associated with the FPDL, resulting in less than 50% of the patients preferring the FPDL despite its more impressive results. We conclude that the final results favor the FPDL over the ATDL for treatments of facial telangiectasia. However, non

  13. Comparison of microemulsion electrokinetic chromatography with high-performance liquid chromatography for fingerprint analysis of resina draconis. (United States)

    Cao, Yuhua; Gong, Wenjun; Li, Nan; Yin, Changna; Wang, Yun


    Microemulsion electrokinetic chromatography (MEEKC) has been developed for fingerprint analysis of resina draconis, a substitute for sanguis draconis in the Chinese market. The microemulsion as the running buffer was made up of 3.3% (w/v) sodium dodecyl sulfate (SDS), 6.6% (w/v) n-butanol, 0.8% (w/v) n-octane, and 10 mmol/L sodium tetraborate buffer (pH 9.2), which was also used as the solvent for ultrasonic extraction of both water- and fat-soluble compounds in the traditional Chinese medicine samples. Four batches of resina draconis obtained from different pharmaceutical factories located in different geographic regions were used to establish the electrophoretic fingerprint. MEEKC was performed using a Beckman PACE/MDQ system equipped with a diode-array detector and with monitoring at 280 nm. The fingerprint of resina draconis comprised 27 common peaks within 100 min. The relative standard deviations of the relative migration time of these common peaks were less than 2.1%. Through repetitive injection of the sample solution six times in 24 h, all relative standard deviations of the migration time and peak area of loureirin A and loureirin B were less than 2.5 and 3.8%, which demonstrated that the method had good stability and reproducibility. The relative peak areas of these common peaks in the electropherograms of four batches of resina draconis were processed with two mathematical methods, the correlation coefficient and the interangle cosine, to valuate the similarity. The values of the similarity degree of all samples were more than 0.91, which showed resina draconis samples from different origins were consistent. On the other hand, high-performance liquid chromatography (HPLC) coupled with photodiode-array detection was also applied to establish the fingerprint of resina draconis. The samples were separated with a LiChrospher C(18) column using acetonitrile (solvent A) and water containing 0.1% H(3)PO(4) (solvent B) as the mobile phase in linear gradient

  14. Making the diagnosis of Sjögren’s syndrome in patients with dry eye

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    Beckman KA


    Full Text Available Kenneth A Beckman,1,2 Jodi Luchs,3,4 Mark S Milner5,6 1Comprehensive EyeCare of Central Ohio, Westerville, OH, 2The Ohio State University, Columbus, OH, 3Hofstra University School of Medicine, Hempstead, NY, 4South Shore Eye Care, Wantagh, NY, 5The Eye Center of Southern Connecticut, PC, Hamden, CT, 6Yale University School of Medicine, New Haven, CT, USA Abstract: Sjögren’s syndrome (SS is a chronic and progressive systemic autoimmune disease that often presents initially with symptoms of dry eye and dry mouth. Symptoms are often non-specific and develop gradually, making diagnosis difficult. Patients with dry eye complaints warrant a step-wise evaluation for possible SS. Initial evaluation requires establishment of a dry eye diagnosis using a combination of patient questionnaires and objective ocular tests, including inflammatory biomarker testing. Additional work-up using the Schirmer test and tear film break-up time can differentiate between aqueous-deficient dry eye (ADDE and evaporative dry eye. The presence of ADDE should trigger further work-up to differentiate between SS-ADDE and non-SS-ADDE. There are numerous non-ocular manifestations of SS, and monitoring for SS-related comorbid findings can aid in diagnosis, ideally in collaboration with a rheumatologist. The clinical work-up of SS can involve a variety of tests, including tear function tests, serological tests for autoantibody biomarkers, minor salivary gland and lacrimal gland biopsies. Examination of classic SS biomarkers (SS-A/Ro, SS-B/La, antinuclear antibody, and rheumatoid factor is a convenient and non-invasive way of evaluating patients for the presence of SS, even years prior to confirmed diagnosis, although not all SS patients will test positive, particularly those with early disease. Recently, newer biomarkers have been identified, including autoantibodies to salivary gland protein-1, parotid secretory protein, and carbonic anhydrase VI, and may allow for earlier

  15. The effect of steam sterilization on the accuracy of spring-style mechanical torque devices for dental implants

    Directory of Open Access Journals (Sweden)

    Mahshid M


    Full Text Available Minoo Mahshid,1 Aboulfazl Saboury,1 Ali Fayaz,1 Seyed Jalil Sadr,1 Friedrich Lampert,2 Maziar Mir2,31Department of Prosthodontics, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2Department of Conservative Dentistry, Aachen RWTH Hospital, Aachen, Germany; 3Beckman Laser Institute, UCI, Irvine, CA, USABackground: Mechanical torque devices (MTDs are one of the most commonly recommended devices used to deliver optimal torque to the screw of dental implants. Recently, high variability has been reported about the accuracy of spring-style mechanical torque devices (S-S MTDs. Joint stability and survival rate of fixed implant supported prosthesis depends on the accuracy of these devices. Currently, there is limited information on the steam sterilization influence on the accuracy of MTDs. The purpose of this study was to assess the effect of steam sterilization on the accuracy (±10% of the target torque of spring-style mechanical torque devices for dental implants.Materials and methods: Fifteen new S-S MTDs and their appropriate drivers from three different manufacturers (Nobel Biocare, Straumann [ITI], and Biomet 3i [3i] were selected. Peak torque of devices (5 in each subgroup was measured before and after autoclaving using a Tohnichi torque gauge. Descriptive statistical analysis was used and a repeated-measures ANOVA with type of device as a between-subject comparison was performed to assess the difference in accuracy among the three groups of spring-style mechanical torque devices after sterilization. A Bonferroni post hoc test was used to assess pairwise comparisons.Results: Before steam sterilization, all the tested devices stayed within 10% of their target values. After 100 sterilization cycles, results didn't show any significant difference between raw and absolute error values in the Nobel Biocare and ITI devices; however the results demonstrated an increase of error values in the 3i group (P < 0.05. Raw error

  16. Evaluation of the Serum Zinc Level in Erosive and Non-Erosive Oral Lichen Planus

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    Gholizadeh N.


    Full Text Available Statement of Problem: Lichen planus is a chronic inflammatory immunologic-based disease involving skin and mucosa. This disease is generally divided into two categories: erosive and non-erosive. Many etiologic factors are deliberated regarding the disease; however, the disorders of immune system and the role of cytotoxic T-lymphocytes and monocytes are more highlighted. Zinc is an imperative element for the growth of epithelium and its deficiency induces the cytotoxic activity of T-helper2 cells which seems to be associated with lichen planus. Purpose: This study aimed to evaluate the levels of serum zinc in erosive and non-erosive oral lichen planus (OLP and compares it with the healthy control group to find out any feasible inference. Materials and Method: A total of 22 patients with erosive oral lichen planus, 22 pa-tients with non erosive OLP and 44 healthy individuals as the control group were recruited in this descriptive-comparative study. All the participants were selected from the referees to the department of oral medicine, school of dentistry, Tabriz University of medical sciences. Serum zinc level was examined for all the individuals with liquid-stat kit (Beckman Instruments Inc., Carlsbad, CA. Data were analyzed by adopting the ANOVA and Tukey tests through SPSS 16 statistical software. Results: The mean age of patients with erosive and non-erosive LP was 41.7 and 41.3 years, respectively. The mean age of the healthy control group was 34.4 years .The mean serum zinc levels in the erosive and non erosive lichen planus groups and control groups were 8.3 (1.15, 11.15 (0.92 and 15.74 (1.75 μg/dl respectively. The difference was statistically significant (p< 0.05. Conclusion: The serum zinc levels were decreased in patients with erosive oral lichen planus. This finding may probably indicate the promising role of zinc in development of oral lichen planus.

  17. Atividade elétrica cerebral do rato com lesões da formação reticular mesencefálica Electrocorticographic study of the rats's bram after lesioning of the midbrain reticular formation

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    Walter C. Pereira


    Full Text Available No presente estudo foram utilizados 73 ratos em preparações agudas e crônicas, nas quais lesamos a formação reticular mesencefálica com corrente contínua (3,5 a 4,0 mA durante 5 a 10 segundos. O eletródio ativo era implantado estereotàxicamente segundo as coordenadas de König e Klippel. As lesões eram feitas parcial ou totalmente, uni ou bilateralmente, e em todos os animais procedeu-se ao controle histológico das áreas lesadas, usando-se o método de Weil. O registro da atividade elétrica cortical foi feito com polígrafo Beckman de 4 canais, utilizando-se derivações bipolares curtas (1mm com eletródios esféricos de platina. As experiências permitiram as seguintes conclusões: 1 — As características eletrofisiológicas dos fusos que ocorrem após lesões da formação reticular mesencefálica são muito semelhantes às dos fusos espontâneos e barbitúricos, inclusive quanto à projeção cortical. Quanto à duração dos potenciais que os constituem, contudo, notamos que a faixa de variação era mais centuada (20 a 80 ms, o que pode ser atribuído à maior complexidade dos potenciais do cérebro isolado, possivelmente pela falta de ação cronadora da formação reticular sobre o sistema sincronizador talâmico. 2 — Os mecanismos envolvidos na gênese dos fusos do sono barbitúrico ou espontâneo e os do cérebro isolado são, pelo menos em parte, dependentes do bloqueio da formação reticular mesencefálica. 3 — A formação reticular mesencefálica ativa preferencialmente o hemisfério cerebral homolateral; o contingente cruzado talvez seja mobilizado somente quando estímulos alertantes intensos atingem o tegmento mesencefálico. 4 — Além da formação reticular mesencefálica deve haver outros mecanismos ativadores corticais, visto que, em preparações agudas de cérebro isolado, observamos: a surtos de curta duração de atividade dessincronizada; b oscilações freqüentes do ECoG durante o registro

  18. Analysis on causes and countermeasures on pseudo - reduction of platelet count by blood cell analyzer%血细胞分析仪计数血小板假性减少原因分析及对策探讨

    Institute of Scientific and Technical Information of China (English)

    李惠卿; 赖馨; 蔡洁丹; 高洪丽; 唐万兵


    Objective To explore the causes and treatment strategies for pseudo - reduction of platelet count by blood cell analyzer. Methods A total of 351 blood samples for platelet count less than 100 × 109 / L measured by Beckman LH780 automatic blood cell analyzer were re - examined by handmade dilute count and smear staining for microscopy. They were divided into 5 groups according to platelet histogram,297 specimens with normal platelet histogram were allocated in group A;13 specimens with small platelet histogram in group B:11 specimens with large platelet histogram in group C:19 specimens with platelet aggregation histogram in group D,and 11 specimens with red cell fragments histo-gram in group E. The platelet count by blood cell analyzer and handmade dilute count were compared with smear staining examination under mi-croscopy for degree of coincidence. Results ① In comparison with handmade dilute count,the difference between group A and group B was not statistically significant( P ﹥ 0. 05),the difference between group C and group D was statistically significant( P < 0. 01),and the platelet count with blood cell analyzer was higher in group E,and the difference between group E and other groups was statistically significant( P < 0. 01). ②There was high degree of coincidence in results between handmade dilution count and smear staining examination under microscopy . Conclusion Platelet count by blood cell analyzer may be pseudo - reduced. In order to raise the accuracy of platelet count,the samples with abnormal platelet histogram need to be checked by handmade dilute count and smear staining examination under microscopy.%目的:探讨血细胞分析仪计数血小板(PLT)假性减少的原因及处理对策。方法对 Beckman LH780自动血细胞分析仪计数 PLT <100×109/ L 的351例样本进行手工稀释计数及涂片染色镜检,按血小板直方图类型分组,A组(正常血小板直方图):297例;B 组

  19. Polymerization time for a microwave-cured acrylic resin with multiple flasks Tempo de polimerização de resina acrílica em microondas, utilizando múltiplas muflas

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    Daniela Maffei Botega


    Full Text Available This study aimed at establishing the polymerization time of a microwave-cured acrylic resin (AcronTM MC, simultaneously processing 2, 4, and 6 flasks. Required time was measured according to the parameters: monomer release in water, Knoop hardness, and porosity. Samples were made with AcronTM MC in different shapes: rectangular (32 x 10 x 2.5 mm for monomer release and porosity; and half-disc (30 mm in diameter x 4 mm in height for Knoop hardness. There were four experimental groups (n = 24 per group: G1 one flask (control; G2 two flasks; G3 four flasks, and G4 six flasks. At first, polymerization protocol was similar for all groups (3 min/450 W. Time was then adjusted for G2, G3, and G4, based on monomer release evaluation in the control group, obtained by spectrophotometer Beckman DU-70, with emitting wave of 206 nm. Knoop hardness test was performed using a Shimadzu HMV 2000 hardness tester, and 10 indentations were performed on each specimen's surface. Porosity was assessed after specimens were immersed in black ink and the pores counted in a microscope. Results showed that the complete polymerization of the resin occurred in 4.5 min for two flasks (G2; 8.5 min for four flasks (G3; and 13 min for six flasks (G4, all with 450 W. Statistical analysis revealed that the number of flasks does not interfere with polymerization, Knoop hardness, and porosity of the resin. Results showed that polymerization of microwave-curing resin with more than one flask is a viable procedure, as long as polymerization time is adjusted.O objetivo deste estudo foi o de determinar os tempos necessários para a polimerização padrão de uma resina acrílica em microondas, utilizando várias muflas simultaneamente. Os tempos necessários foram aferidos por parâmetros como monômeros liberados em água, dureza Knoop e porosidade. As amostras, confeccionadas em resina AcronTM MC, apresentavam as seguintes dimensões: para os parâmetros monômero residual e porosidade

  20. Benzene activates caspase-4 and -12 at the transcription level, without an association with apoptosis, in mouse bone marrow cells lacking the p53 gene

    Energy Technology Data Exchange (ETDEWEB)

    Yi, Jung-Yeon; Han, Jeong-Hee; Yoon, Byung-Il [Kangwon National University, School of Veterinary Medicine, Chuncheon, Gangwon (Korea); Hirabayashi, Yoko; Kodama, Yukio; Kanno, Jun [National Institute of Health Sciences, Division of Cellular and Molecular Toxicology, Center for Biological Safety and Research, Tokyo (Japan); Choi, Yang-Kyu [Konkuk University, College of Veterinary Medicine, Seoul (Korea); Inoue, Tohru [National Institute of Health Sciences, Biological Safety and Research Center, Tokyo (Japan)


    Benzene is a well-known environmental pollutant that can induce hematotoxicity, aplastic anemia, acute myelogenous leukemia, and lymphoma. However, although benzene metabolites are known to induce oxidative stress and disrupt the cell cycle, the mechanism underlying lympho/leukemogenicity is not fully understood. Caspase-4 (alias caspase-11) and -12 are inflammatory caspases implicated in inflammation and endoplasmic reticulum stress-induced apoptosis. The objectives of this study were to investigate the altered expression of caspase-4 and -12 in mouse bone marrow after benzene exposure and to determine whether their alterations are associated with benzene-induced bone marrow toxicity, especially cellular apoptosis. In addition, we evaluated whether the p53 gene is involved in regulating the mechanism, using both wild-type (WT) mice and mice lacking the p53 gene. For this study, 8-week-old C57BL/6 mice [WT and p53 knockout (KO)] were administered a benzene solution (150 mg/kg diluted in corn oil) via oral gavage once daily, 5 days/week, for 1 or 2 weeks. Blood and bone marrow cells were collected and cell counts were measured using a Coulter counter. Total mRNA and protein extracts were prepared from the harvested bone marrow cells. Then qRT-PCR and Western blotting were performed to detect changes in the caspases at the mRNA and protein level, respectively. A DNA fragmentation assay and Annexin-V staining were carried out on the bone marrow cells to detect apoptosis. Results indicated that when compared to the control, leukocyte number and bone marrow cellularity decreased significantly in WT mice. The expression of caspase-4 and -12 mRNA increased significantly after 12 days of benzene treatment in the bone marrow cells of benzene-exposed p53KO mice. However, apoptosis detection assays indicated no evidence of apoptosis in p53KO or WT mice. In addition, no changes of other apoptosis-related caspases, such as caspase-3 and -9, were found in WT or p53KO mice at the

  1. Physical and Chemical Characteristics of Desert Dust Deposited on Mt. Elbrus, Caucasus as Documented in Snow Pit and Shallow Core Records (United States)

    Kutuzov, S.; Shahgedanova, M.; Mikhalenko, V.; Ginot, P.; Lavrentiev, I.; Popov, G.


    We present a study of dust deposition events and its physical and chemical characteristics in Caucasus Mountains as documented by snow and firn pack at Mt Elbrus. Dust samples were collected from the shallow ice cores and snow pits in 2009-2013 at the western Elbrus plateau (5150 m a.s.l.). Particle size distribution and chemical analysis (major ions, trace elements) were completed for each sample using Coulter Counter Multisizer III, scanning electron microscopy (SEM), IC and ICPMS analysis. It was shown that desert dust deposition occurred in Caucasus 4-8 times a year and originates from the Northern Sahara and the deserts of the Middle East. Analysis of volumetric particle size distributions showed that the modal values ranged between 2 μm and 4 μm although most samples were characterised by modal values of 2.0-2.8 μm with an average of 2.6 μm. These values are lower than those obtained from the ice cores in central and southern Asia following the deposition of long-travelled dust and are closer to those reported for the European Alps and the polar ice cores. All samples containing dust have a single mode which is usually interpreted as a single source region. They do not reveal any significant differences between the Saharan and the Middle Eastern sources. The annual average dust mass concentrations were 10-15 mg kg-1 which is higher than the average concentrations reported for other mountain regions and this was strongly affected by dust deposition events. The deposition of dust resulted in elevated concentrations of most ions, especially Ca2+, Mg2+, K+, and sulphates. Dust originated from multiple sources in the Middle East including Mesopotamia or passing over the Middle East was characterised by the elevated concentrations of nitrates and ammonia which is related to a high atmospheric loads of ammonium emitted by agricultural sources and high concentrations of ammonium in dust originating from this region. By contrast, samples of the Saharan dust showed

  2. Effect of chronic alcohol consumption on the red blood cell count and RBC indices in the HIV infected patients on d4T/3TC/NVP drug regimen in Uganda

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    Godfrey S. Bbosa


    Full Text Available Alcohol consumption is common problem in Uganda. Among the types of alcohols consumed include beers, spirits, liqueurs, wines and traditional brew. These alcohols are easily accessible and consumed by many people including the HIV infected patients who are on the d4T/3TC/NVP regimen. The aim of this study was to determine the effect of chronic alcohol intake on the red blood cell count (RBC and the RBC indices in the HIV-infected patients on d4T/3TC/NVP regimen. It was a case control study that used a repeated measures design model where serial measurements of the red blood cell count (RBC and RBC indices were determined at 3 month interval for 9 months. A total of 41 HIV infected patients were recruited and grouped into two arms; the control group had 21 patients and the chronic alcohol group had 20 patients. The RBC and RBC indices of the whole blood were determined using automated hematological Coulter CBC-5 Hematology Analyzer system using standard procedures. The data was sorted into alcohol-use self reporting by WHO AUDIT tool and alcohol-use biomarkers groups. It was analysed using the SAS 2003 version 9.1 statistical package with the repeated measures fixed model. The means were compared using the student t-test. The mean MCV and MCH values in the chronic alcohol use group were higher than in the control group and there was a significant difference between the 2 groups (p<0.05 for both the WHO AUDIT tool group and chronic alcohol use biomarkers group. The mean RBC count, Hct, HGB and MCHC values in both the control and chronic alcohol use groups were within the normal reference ranges for both groups though the trend was lower in alcohol group. Chronic alcohol use affects the RBC and RBC indices in the HIV infected patients on d4T/3TC/NVP treatment regimen. [Int J Basic Clin Pharmacol 2013; 2(5.000: 528-536

  3. The Role of Aerobic and Anaerobic Training Programs on CD34+ Stem Cells and Chosen Physiological Variables (United States)

    Shalaby, Mohammed Nader; Saad, Mohammed; Akar, Samy; Reda, Mubarak Abdelreda Ali; Shalgham, Ahmed


    Exercise is one of the most powerful non-pharmacological strategies, which can affect nearly all cells and organs in the body. Changes in the behavior of adult stem cells have been shown to occur in response to exercise. Exercise may act on regenerative potential of tissues by altering the ability to generate new stem cells and differentiated cells that are able to carry out tissue specific functions. The purpose of this study was to reveal the role of aerobic and anaerobic training programs on CD34+ Stem Cells and chosen physiological variables. Twenty healthy male athletes aged 18–24 years were recruited for this study. Healthy low active males and BMI matched participants (n=10) aged 20–22 years were recruited as controls. Aerobic and anaerobic training programs for 12 weeks were conducted. VO2max pulse observation was carried out using the Astrand Rhyming protocol. RBCs, WBCs, HB and hematocrit were estimated using a coulter counter, lactate by the Accusport apparatus, CD34+ stem cells by flow cytometry. VO2max was increased significantly in case of the aerobic training program compared to anaerobic one (62±2.2 ml/kg/min vs. 54±2.1 ml/kg/min). Haemotological values increased significantly in the anaerobic program when compared to the aerobic one, RBCs (5.3±0.3 and 4.9±0.2 mln/ul), WBCs (6.6±0.5 and 6.1±0.4 thous/ul), HB (15.4±0.4 and 14.2±0.5 g/de), Hematocrit (4.6±1.2 and 4.4±1.1 %), CD34+ stem cells count increased significantly in case of the anaerobic program compared to the aerobic (251.6±21.64 and 130±14.61) and sedentary one (172±24.10). These findings suggest that anaerobic training programs provoke better adaptation to exercise and stem cell counts may differ between trained and sedentary subjects. Circulating immature cells are likely to be involved in angiogenesis and repair process, both mechanisms being associated with strenuous exercise. Knowledge of the physiological effects of training on stem cells might be of potential

  4. The multi-xenobiotic resistance (MXR) efflux activity in hemocytes of Mytilus edulis is mediated by an ATP binding cassette transporter of class C (ABCC) principally inducible in eosinophilic granulocytes. (United States)

    Rioult, Damien; Pasquier, Jennifer; Boulangé-Lecomte, Céline; Poret, Agnès; Abbas, Imane; Marin, Matthieu; Minier, Christophe; Le Foll, Frank


    In marine and estuarine species, immunotoxic and/or immunomodulatory mechanisms are the crossroad of interactions between xenobiotics, microorganisms and physicochemical variations of the environment. In mussels, immunity relies exclusively on innate responses carried out by cells collectively called hemocytes and found in the open hemolymphatic circulatory system of these organisms. However, hemocytes do not form a homogenous population of immune cells since distinct subtypes of mussel blood cells can be distinguished by cytochemistry, flow cytometry or cell motility analysis. Previous studies have also shown that these cells are able to efflux xenobiotics by means of ATP binding cassette (ABC) transporter activities conferring a multixenobiotic resistance (MXR) phenotype. ABC transporters corresponding to vertebrate class B/P-glycoprotein (P-gp) and to class C/multidrug resistance related protein (MRP) are characterized in Mytilidae. Herein, we have investigated the relative contributions of ABCB- and ABCC-mediated efflux within the different hemocyte subpopulations of Mytilus edulis mussels, collected from areas differentially impacted by chemical contaminants in Normandy (France). RT-PCR analyses provide evidence for the presence of ABCB and ABCC transporters transcripts in hemocytes. Immunodetection of ABCB/P-gp with the monoclonal antibody UIC2 in living hemocytes revealed that expression was restricted to granular structures of spread cells. Efflux transporter activities, with calcein-AM as fluorescent probe, were measured by combining flow cytometry to accurate Coulter cell size measurements in order to get a cell-volume normalized fluorescence concentration. In these conditions, basal fluorescence levels were higher in hemocytes originating from Yport (control site) than in cells collected from the harbor of Le Havre, where mussels are more exposed to with persistent pollutants. By using specific ABCB/P-gp (verapamil, PSC833, zosuquidar) and ABCC/MRP (MK

  5. Effectiveness of a physiotherapy-initiated telephone triage of orthopedic waitlist patients

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    Gilmore L


    Full Text Available Joanne Morris1, Karen Grimmer-Somers2, Saravana Kumar2, Karen Murphy3, Lisa Gilmore1, Bryan Ashman1, Chandima Perera1, Kathryn Vine1, Corinne Coulter11The Canberra Hospital, ACT Government Health Directorate, Canberra, ACT, Australia; 2International Centre for Allied Health Evidence, University of South Australia, Adelaide, SA, Australia; 3ACT Government Health Directorate, Canberra, ACT, AustraliaBackground: There is generally a lengthy wait on outpatient orthopedic waiting lists in Australian public hospitals to consult a specialist. Patients then wait again for surgery, if required. Patients with higher need are rarely prioritized, and there is the potential for increased morbidity for those who wait. There is generally no option of alternative care whilst waiting. This paper compares historical orthopedic outpatient clinic data with the outcomes of a physiotherapy-led initiative in one large Australian tertiary hospital.Methods: Two physiotherapists working within-scope conducted a telephone triage (October to December 2010 using a standard instrument for all new patients on the orthopedic waiting list. They were offered primary treatment options of retaining their appointment, being discharged, referral to a new model of assessment (multidisciplinary specialist clinic, or referral to physiotherapy. The outcomes were costs of the service, waiting time, and percentage of patients taking up management options. This was compared with a historical sample of new patients on the orthopedic waiting list (January to March 2009, whose treatment consumption was tracked longitudinally.Results: The telephone triage resulted in 16.4% patients being discharged directly (compared with 0.1% comparison sample. For approximately AU$17.00 per patient, the telephone triage process released 21 booked appointments on the outpatient clinic waiting list. Moreover, approximately 26% patients were referred directly to physiotherapy, which was not a primary management

  6. Effects of CO2 on particle size distribution and phytoplankton abundance during a mesocosm bloom experiment (PeECE II

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    M. Schartau


    Full Text Available The influence of seawater carbon dioxide (CO2 concentration on the size distribution of suspended particles (2–60 μm and on phytoplankton abundance was investigated during a mesocosm experiment at the large scale facility (LFS in Bergen, Norway, in the frame of the Pelagic Ecosystem CO2 Enrichment study (PeECE II. In nine outdoor enclosures the partial pressure of CO2 in seawater was modified by an aeration system to simulate past (~190 μatm CO2, present (~370 μatm CO2 and future (~700 μatm CO2 CO2 conditions in triplicates. Due to the initial addition of inorganic nutrients, phytoplankton blooms developed in all mesocosms and were monitored over a period of 19 days. Seawater samples were collected daily for analysing the abundance of suspended particles and phytoplankton with the Coulter Counter and with Flow Cytometry, respectively. During the bloom period, the abundance of small particles (2 levels. At that time, a direct relationship between the total-surface-to-total-volume ratio of suspended particles and DIC concentration was determined for all mesocosms. Significant changes with respect to the CO2 treatment were also observed in the phytoplankton community structure. While some populations such as diatoms seemed to be insensitive to the CO2 treatment, others like Micromonas spp. increased with CO2, or showed maximum abundance at present day CO2 (i.e. Emiliania huxleyi. The strongest response to CO2 was observed in the abundance of small autotrophic nano-plankton that strongly increased during the bloom in the past CO2 mesocosms. Together, changes in particle size distribution and phytoplankton community indicate a complex interplay between the ability of the cells to physiologically respond to changes in CO2 and size selection. Size of cells is of general importance for a variety of processes in marine systems such as diffusion-limited uptake of substrates, resource allocation, predator-prey interaction, and gravitational settling

  7. First cytochemical study of haemocytes from the crab Carcinus aestuarii (Crustacea, Decapoda

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    V. Matozzo


    Full Text Available For the first time, a morphological study of haemocytes from the crab Carcinus aestuarii was carried out by means of light microscopy and differing cytochemical assays. Analysis of haemocyte size frequency distribution (performed by means of a Coulter Counter revealed the presence of two distinct haemocyte fractions in C. aestuarii haemolymph, depending on cell size. The first fraction was of about 3-5 µm in diameter and 30-50 fL in volume, the second was of about 6-12 µm in diameter and over 200 fL in volume. Mean cell diameter and volume were 8.20±1.7 µm and 272.30±143.5 fL, respectively. Haemocytes observed under light microscope were distinguished in three cell types: granulocytes (28%; 11.94±1.43 µm in diameter with evident cytoplasmic granules, semigranulocytes (27%; 12.38±1.76 µm in diameter with less granules than granulocytes, and hyalinocytes (44%; 7.88±1.6 µm in diameter without granules. In addition, a peculiar cell type was occasionally found (about 1%: it was 25-30 µm in diameter and had a great vacuole and a peripheral cytoplasm with granules. Granulocyte and semigranulocyte granules stained in vivo with Neutral Red, indicating that they were lysosomes. Giemsa’s dye confirmed that granulocytes and semigranulocytes were larger than hyalinocytes. Pappenheim’s panoptical staining and Ehrlich’s triacid mixture allowed to distinguish granule-containing cells (including semigranulocytes in acidophils (64%, basophils (35% and neutrophils (1%. Hyalinocytes showed always a basophilic cytoplasm. Haemocytes were positive to the PAS reaction for carbohydrates, even if cytoplasm carbohydrate distribution varied among cell types. Lastly, lipids were found on cell membrane and in cytoplasm of all haemocyte types in the form of black spots produced after Sudan Black B staining. The morphological characterisation of C. aestuarii haemocytes by light microscopy was necessary before performing both ultrastructural and functional

  8. 不同品牌化学发光仪检测甲状腺激素的相关性分析

    Institute of Scientific and Technical Information of China (English)

    谭云昌; 曾正莲


    目的 探讨两台不同品牌化学发光免疫分析仪检测结果的一致性.方法 每天患者标本采用Bechman Coulter ACCESS2检测后,根据仪器的游离三碘甲腺原氨酸(FT3)正常参考范围选择正常和异常各一标本采用Abbott Axsm Symtem进行检测,正常、异常标本各30份,记录用两种方法检测的数据.结果 30份FT3正常标本和30份FT3异常标本分别在2个不同检测系统测定结果方差齐,经配伍组设计资料的方差分析,各组间的差异有统计学意义(P<0.05),可靠性系数@=0.990,2个检测系统间FT3、游离甲状腺素(FT4)、促甲状腺素(TSH)的相关系数均大于0.970.结论 两台不同仪器间的测定结果差异有统计学意义,通过选择一台参加湖北省临床检验中心和卫生部临床检验中心内分泌项目获得室间质评成绩优秀的仪器作为参考仪器,配制出与另外一台仪器定标液浓度相同的血清作为校准品来校正另外一台仪器的方法,结果表明,两台仪器测定患者标本结果差异无统计学意义,比对试验符合临床要求.

  9. Interstitial diffuse radiance spectroscopy of gold nanocages and nanorods in bulk muscle tissues

    Directory of Open Access Journals (Sweden)

    Grabtchak S


    Full Text Available Serge Grabtchak,1,2 Logan G Montgomery,1 Bo Pang,3,4 Yi Wang,4,5 Chao Zhang,6,7 Zhiyuan Li,6,7 Younan Xia,4,8 William M Whelan1,91Department of Physics, University of Prince Edward Island, Charlottetown, PEI, Canada; 2Departments of Electrical and Computer Engineering, and Physics, Dalhousie University, Halifax, Canada; 3Department of Biomedical Engineering, Peking University, Beijing, People’s Republic of China; 4The Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA; 5Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing, People’s Republic of China; 6Laboratory of Optical Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, People’s Republic of China; 7College of Physics and Optoelectronics, South China University of Technology, Guangzhou, People’s Republic of China; 8School of Chemistry and Biochemistry, and School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, USA; 9Atlantic Veterinary College, Charlottetown, PEI, CanadaAbstract: Radiance spectroscopy was applied to the interstitial detection of localized inclusions containing Au nanocages or nanorods with various concentrations embedded in porcine muscle phantoms. The radiance was quantified using a perturbation approach, which enabled the separation of contributions from the porcine phantom and the localized inclusion, with the inclusion serving as a perturbation probe of photon distributions in the turbid medium. Positioning the inclusion at various places in the phantom allowed for tracking of photons that originated from a light source, passed through the inclusion’s location, and reached a detector. The inclusions with high extinction coefficients were able to absorb nearly all photons in the range of 650–900 nm, leading to a spectrally flat radiance signal. This signal could be

  10. Cytometric evaluation of intracellular IFN-γ and IL-4 levels in thyroid follicular cells from patients with autoimmune thyroid diseases

    Directory of Open Access Journals (Sweden)

    Bossowski Artur


    Full Text Available Abstract Background In recent few years is underlined that altered balance of pro- and anti-inflammatory cytokines play an important role in the pathogenesis of AITD. The aim of this study was to estimate intracellular INF-γ and IL-4 levels in thyroid-infiltrating lymphocytes and thyrocytes isolated from thyroid tissues in 54 adolescent patients aged 8-21 years, with Graves' disease (GD; n = 18, Hashimoto's thyroiditis (HT; n = 18 and non-toxic multinodular goiter (NTMG; n = 18. Methods Fresh thyroid tissues were taken on culture medium RPMI -1640, it was mechanically prepared. In next step were added cell activators -12- myristate 13- the acetate (PMA and Ionomycin as well as the inhibitor of transportation of proteins - Breferdin A. They were cultured 24 hours in 50 ml flasks at 37°C in a 5-95% CO2-air water-saturated atmosphere. After that, thyrocytes were identified by mouse mAb directed against human TPO epitope 64 conjugated with rabbit anti-mouse antibodies IgG (Fab'2 labeled by FITC. After incubation at room temperature to each of samples added reagent A fixative the cellular membrane. In next step into the cell suspensions were added reagent B to permeabilization of cellular membrane and specific anti-IL-4-PE or anti-IFN-γ-PE mAbs. Identification of intracellular cytokines in T lymphocytes was performed in the same procedure with application of anti-CD4-PerCP and anti-CD8-PerCP mAbs specific for T lymphocytes. The cells were analyzed in a flow cytometry (Coulter EPICS XL. Results In examined group of patients with GD we observed statistically significant higher mean percentage of cells with phenotype CD4+IL-4 (p Conclusions We conclude that human thyrocytes in autoimmune thyroid disorders could be a source of cytokine production and that their activation influences local interaction with T lymphocytes inflowing to the thyroid gland.

  11. Cell type-dependent uptake, localization, and cytotoxicity of 1.9 nm gold nanoparticles

    Directory of Open Access Journals (Sweden)

    Coulter JA


    Full Text Available Jonathan A Coulter,1 Suneil Jain,2 Karl T Butterworth,2 Laura Taggart,2 Glenn Dickson,2 Stephen J McMahon,3 Wendy Hyland,1 Mark F Muir,3 Coleman Trainor,2 Alan Hounsell,2,4 Joe M O'Sullivan,2,4 Giuseppe Schettino,2 Fred Currell,3 David G Hirst,1 Kevin M Prise21School of Pharmacy, McClay Research Centre, 2Centre for Cancer Research and Cell Biology, 3School of Mathematics and Physics, Queens University Belfast, 4Belfast Health and Social Care Trust, Belfast, IrelandBackground: This follow-up study aims to determine the physical parameters which govern the differential radiosensitization capacity of two tumor cell lines and one immortalized normal cell line to 1.9 nm gold nanoparticles. In addition to comparing the uptake potential, localization, and cytotoxicity of 1.9 nm gold nanoparticles, the current study also draws on comparisons between nanoparticle size and total nanoparticle uptake based on previously published data.Methods: We quantified gold nanoparticle uptake using atomic emission spectroscopy and imaged intracellular localization by transmission electron microscopy. Cell growth delay and clonogenic assays were used to determine cytotoxicity and radiosensitization potential, respectively. Mechanistic data were obtained by Western blot, flow cytometry, and assays for reactive oxygen species.Results: Gold nanoparticle uptake was preferentially observed in tumor cells, resulting in an increased expression of cleaved caspase proteins and an accumulation of cells in sub G1 phase. Despite this, gold nanoparticle cytotoxicity remained low, with immortalized normal cells exhibiting an LD50 concentration approximately 14 times higher than tumor cells. The surviving fraction for gold nanoparticle-treated cells at 3 Gy compared with that of untreated control cells indicated a strong dependence on cell type in respect to radiosensitization potential.Conclusion: Gold nanoparticles were most avidly endocytosed and localized within cytoplasmic

  12. Role of Tamm-Horsfall protein and uromodulin in calcium oxalate crystallization

    Directory of Open Access Journals (Sweden)

    Carvalho M.


    Full Text Available One of the defenses against nephrolithiasis is provided by macromolecules that modulate the nucleation, growth, aggregation and retention of crystals in the kidneys. The aim of the present study was to determine the behavior of two of these proteins, Tamm-Horsfall and uromodulin, in calcium oxalate crystallization in vitro. We studied a group of 10 male stone formers who had formed at least one kidney stone composed of calcium oxalate. They were classified as having idiopathic nephrolithiasis and had no well-known metabolic risk factors involved in kidney stone pathogenesis. Ten normal men were used as controls, as was a group consisting of five normal women and another consisting of five pregnant women. Crystallization was induced by a fixed supersaturation of calcium oxalate and measured with a Coulter Counter. All findings were confirmed by light and scanning electron microscopy. The number of particulate material deposited from patients with Tamm-Horsfall protein was higher than that of the controls (P<0.001. However, Tamm-Horsfall protein decreased the particle diameter of the stone formers when analyzed by the mode of the volume distribution curve (P<0.002 (5.64 ± 0.55 µm compared to 11.41 ± 0.48 µm of uromodulin; 15.94 ± 3.93 µm and 12.45 ± 0.97 µm of normal men Tamm-Horsfall protein and uromodulin, respectively; 8.17 ± 1.57 µm and 9.82 ± 0.95 µm of normal women Tamm-Horsfall protein and uromodulin, respectively; 12.17 ± 1.41 µm and 12.99 ± 0.51 µm of pregnant Tamm-Horsfall protein and uromodulin, respectively. Uromodulin produced fewer particles than Tamm-Horsfall protein in all groups. Nonetheless, the total volume of the crystals produced by uromodulin was higher than that produced by Tamm-Horsfall protein. Our results indicate a different effect of Tamm-Horsfall protein and uromodulin. This dual behavior suggests different functions. Tamm-Horsfall protein may act on nucleation and inhibit crystal aggregation, while

  13. Science education through open and distance learning at Higher Education level

    Directory of Open Access Journals (Sweden)

    Amrita NIGAM


    Full Text Available Abstract The changes faced by the society in the past few decades brought revolution in all areas. The job requirements have undergone change tremendously. The emergence of e-culture, e-education, e-governance, e-training, e-work sites and so on questioned the capacity of conventional face to face education in catering to all and relevance of existing job related skills to a great extent in the emerging global society. Today, every one has to update his/her educational and/or professional skills and competencies to cope up with the emerging work challenges. This is more so in the field of science and technology. At the same time, it is impossible to cater to educational and training opportunities to one and all those who aspire for it through the conventional set up. The distance and open learning (ODL seems to be one of the viable alternatives. Today, the success and viability of ODL is accepted globally. Coulter (1989, through a study demonstrated that ODL is a cost-effective medium in providing educational opportunities. Similarly Holmberg (1981 also mentioned ODL as a systematic teaching-learning medium by using variety of medium for imparting learning. The present study is an attempt to study the experiences of the open science learners of IGNOU on different aspect of the science higher education. Here a questionnaire was used to collect the data and responses from 81 students enrolled for B. Sc. from IGNOU were collected. The findings of the study reported that society has undergone drastic changes in the last few decades. The revolution led due to Information and Communication Technologies (ICTs have widely affected all aspects of society. The emerging jobs require entirely new skills and competencies i.e., employment in BPOs or switching over to e-governance, e-Banking and e- based sectors. Even e-learning has made numerous expectations from teachers and other personnel. The use of ICTs in almost every field needs adequately trained

  14. Does chronic alcohol use by HIV-infected patients on d4T/3TC/NVP drug regimen effect the HIV viral load and what is the therapeutic window of the drugs, CD4+ count and WBC count in patients with high viral load during the 9 months period of follow up?

    Directory of Open Access Journals (Sweden)

    Godfrey S. Bbosa


    Full Text Available The study investigated the effects of chronic alcohol use on HIV viral load in HIV-infected patients on d4T/3TC/NVP drug regimen during 9 months follow up period. It also determined plasma drug concentrations of d4T, 3TC and NVP; CD4+ and WBC counts for patients with high HIV viral load. A case-control study using repeated measures with serial measurements was used. A total of 41 patients (20 alcohol group and 21 control group were screened for alcohol use using WHO AUDIT tool and chronic alcohol use biomarkers. Blood sampling was done at 3 month intervals for a period of 9 months. HIV viral load was determined using Roche Amplicor HIV-1 monitor test, version 1.5 (Amplicor. The d4T, 3TC and NVP concentrations were determined by Shimadzu Class-VPTM HPLC Chromatography data system version 6.1. The CD4+ cell count was determined using FACSCalibur flow cytometer. The WBC was determined using automated hematological Coulter CBC-5 Hematology Analyzer system. Results show that % patients with HIV viral load ≥400 copies/ml in control group was highest (23.8%, n=5 at 3 month while in chronic alcohol use group, it was at 0 month (35%, n=7 for both WHO AUDIT tool and chronic alcohol-use biomarkers groups. Generally patients with high viral load ≥400 copies/ml was observed in chronic alcohol use as compared to control group in both WHO AUDIT tool and biomarkers group despite of patients having high steady state d4T, 3TC and NVP plasma drug concentrations in circulation that is available to suppress HIV virus. The high viral load could be associated with the emergence of resistance of the HIV virus and these patients generally had a low CD4+ cell count. Some of these patients had no detectable d4T plasma drug concentrations in circulation and most of them with high viral load had sub-therapeutic NVP plasma drug concentrations in their blood circulation. Chronic ethanol use by HIV-infected patients on d4T/3TC/NVP drug regimen increased HIV viral load and

  15. Influence of p53 abrogation on penclomedine and radiation-induced cell kill

    International Nuclear Information System (INIS)

    Purpose: Penclomedine [(3.5-dichloro-2,4-dimethoxy-6-trichloromethyl)pyridine], a synthetic pyridine derivative, has documented activity against human and murine tumor cells and is currently being investigated in Phase I clinical trials. Cells exposed to Penclomedine reveal abundant chromosomal aberrations suggesting that its mechanism of action may be through DNA alkylation. Since p53 is considered to be a DNA damage responsive element, we investigated the influence of p53 abrogation on Penclomedine sensitivity, on sensitivity to ionizing radiation and to both modalities combined. Material and Methods: Three malignant cell lines, derived from a human colorectal carcinoma, were used in this study: RKO cells which contain wild-type p53 alleles, RKO cells transfected with an over expressed mutant p53 transgene (p53.13) and RKO cells transfected with an over expressed HPV-16 E6 gene (RC 10.03) resulting in abrogation of normal p53 function. Cells were exposed to increasing concentrations of Penclomedine (0-120 μg/ml) for variable periods of time (4, 6, 8, 12, 24 hours) as well as to graded doses of ionizing radiation (0-8 Gy), either alone or following 24 hour exposure to Penclomedine. Cell viability was monitored by the colony-forming assay and Trypan blue exclusion. Cell numbers were counted using a hemocytometer and Coulter counter. Both asynchronous and synchronized cell populations were studied with synchrony attained by mitotic shake off. S phase cell determination was by anti-BrdU immuno labeling. Results: Each cell line exhibited a dose and time-dependent reduction in cell viability following Penclomedine treatment. However, RC 10.03 cells showed a dramatic enhancement in cell kill compared to the parental RKO cells (P<0.05). No significant difference in radiation sensitivity amongst the 3 cell lines was observed when cells were exposed either as an asynchronous population or in early G1. Conclusion: The results of this study suggest that radiation

  16. Atributos químicos de um latossolo bruno sob sistema plantio direto em função da estratégia de adubação e do método de amostragem de solo Chemical properties of a haplohumox under no-tillage as related to fertilization strategies and soil sampling method

    Directory of Open Access Journals (Sweden)

    Volnei Pauletti


    greater in the no-tillage system because there is no homogenization of the soil surface layer. This study evaluated the effect of fertilization strategies on soil chemical properties at distinct soil depths after three and six years from the beginning of the experiment in a Haplohumox under long-term no-tillage. Besides, the variation of these properties considering two methods of soil sampling, with and without inclusion of the seeding row, was evaluated. Ten treatments were applied, nine considering P sources (rock phosphate and triple superphosphate, row preparation equipment (double disc opener and coulter opener, fertilizer placement (in-row and broadcast, time of application (summer and winter and one control treatment, without fertilizer. The measured soil chemical properties were higher in the surface layers and depth distribution, except for P, was not influenced by the fertilization strategies. The soil P values were the lowest in the 0-5 cm layer where the coulter opener equipment had been continuously used for more than three years and no fertilizer was applied. With the inclusion of the sowing row in the soil sampling, higher K, Ca and CEC values were observed. The methodologies of soil sampling, with and without the inclusion of the sowing row and the 0-10 and 0-20 cm sampling layers resulted in similar fertilizer and lime recommendations for areas under long-term no-tillage.


    Institute of Scientific and Technical Information of China (English)

    施一鸣; 单国荣


    The surfactant-free emulsion polymerization ( SFEP) of styrene and methyl methacrylate carried out in the aqueous solution of poly ( ethylene glycol) (PEG) was studied. With high power optical microscope, Coulter counter and transmission electron microscopy, particle size, particle size distribution and zeta potential were measured in the process of polymerization. It is interesting to observe that the polymer particle size experiences a process from small to large,which can be more than 1000 μm,and then from large to small, which is back to a range of 0. 04 - 0. 18 μm. When the particle size returns to nanometer scale, the system has an obvious temperature rising. This phenomenon is different from the traditional nucleation mechanism of SFEP. The particle, which is formed by the homogeneous nucleation or oligomer micelle nucleation mechanism that is popularly received as traditional nucleation mechanism of SFEP, usually has a trend to become bigger and bigger to the end in the process of SFEP. It is obviously found that the freshly formed particles are first coalesced to form a particle pile,which is bigger than 1000 pun and even seen with eyes,and then corrupted into tinny ones, which are smaller than 0. 04 u,m and even beyond the ability of high power optical microscope. With transmission electron microscopy (TEM), it was clearly proven the existence of paricle pile which was composed of a lot of little particles ranging from 40 nm to 80 nm. And by use of Coulter zeta potential measurement, it was found that the zeta potential of particles had a trend from low to high level, and experenced abruptly increase in zeta potential at 60 min which was just the time that the little particle seperated from the paricle pile. This phenomenan was very different from the system that SEFP was carryed out in water without PEG, in which the zeta potential of particle was keeping about -50 mV and just changed a little after 50 min. It was reasonble to think that the potential

  18. Métodos de detección de la mastitis bovina (Methods of detection of the bovine mastitis

    Directory of Open Access Journals (Sweden)

    Bedolla, CC


    Full Text Available ResumenEste artículo es una revisión bibliográfica sobre los métodos de detección de la mastitis bovina que se utilizan más comúnmente en el mundo. La mastitis bovina es la inflamación de la glándula mamaria causada por una infección por patógenos. Es una de las enfermedades más frecuentes de la producción que afecta a la industria lechera en todo el mundo. Puede presentarse de manera clínica y subclínica. La mastitis subclínica es de larga duración y es mucho más frecuente que la mastitis clínica. Dentro de los métodos que se usan con mayor frecuencia a nivel de campo para diagnosticar mastitis clínicas, se encuentran el método de observación y palpación de la ubre y las pruebas físicas, como la prueba de escudilla de ordeño, prueba del paño negro y taza probadora. Las pruebas químicas, como la prueba de conductividad eléctrica de la leche, papel indicador de mastitis y prueba de whiteside que sirven también para diagnosticar mastitis clínicas y subclínicas. Las pruebas biológicas, como son la prueba de California para mastitis, la prueba de Wisconsin, el diagnóstico bacteriológico por los métodos de aislamiento, cultivo, tinción, pruebas bioquímicas e identificación y el conteo de células somáticas por microscopia directa y el somaticell. Otros métodos utilizados actualmente por su rapidez y efectividad son los electrónicos como el fossomatic y el counter coulter, los cuales tienen una aplicación universal sobre todo en laboratorios de control lechero o dedicados al diagnóstico e investigación de la mastitis y el DeLaval cell counter. Los métodos de detección de mastitis son una herramienta que permite identificar el tipo de infección clínica o subclínica que puede presentarse dentro de un hato lechero, por lo que el método que se elija para determinar las pruebas será esencial para tener un diagnóstico más preciso.AbstractThis article is a bibliographical revision on the methods of

  19. Evaluation of Innotrac Aio! Second-Generation Cardiac Troponin I Assay: The Main Characteristics for Routine Clinical Use

    Directory of Open Access Journals (Sweden)

    M. Puukka


    Full Text Available The availability of a simple, sensitive, and rapid test using whole blood to facilitate processing and to reduce the turnaround time could improve the management of patients presenting with chest pain. The aim of this study was an evaluation of the Innotrac Aio! second-generation cardiac troponin I (cTnI assay. The Innotrac Aio! second-generation cTnI assay was compared with the Abbott AxSYM first-generation cTnI, Beckman Access AccuTnI, and Innotrac Aio! first-generation cTnI assays. We studied serum samples from 15 patients with positive rheumatoid factor but with no indication of myocardial infarction (MI. Additionally, the stability of the sample with different matrices and the influence of hemodialysis on the cTnI concentration were evaluated. Within-assay CVs were 3.2%–10.9%, and between-assay precision ranged from 4.0% to 17.2% for cTnI. The functional sensitivity (CV=20% and the concentration giving CV of 10% were approximated to be 0.02 and 0.04, respectively. The assay was found to be linear within the tested range of 0.063–111.6 μg/L. The correlations between the second-generation Innotrac Aio!, Access, and AxSYM cTnI assays were good (r coefficients 0.947–0.966, but involved differences in the measured concentrations, and the biases were highest with cTnI at low concentrations. The second-generation Innotrac Aio! cTnI assay was found to be superior to the first-generation assay with regard to precision in the low concentration range. The stability of the cTnI level was best in the serum, lithium-heparin plasma, and lithium-heparin whole blood samples (n=10, decrease <10% in 24 hours at +20°C and at +4°C. There was no remarkable influence of hemodialysis on the cTnI release. False-positive cTnI values occurred in the presence of very high rheumatoid factor values, that is, over 3000 U/L. The 99th percentile of the apparently healthy reference group was ≤0.03Ã

  20. 2702例孕妇中期胎儿唐氏征筛查结果的分析%Screening Analysis of Down's Syndromes in 2702 cases on Middle Period Pregnancy

    Institute of Scientific and Technical Information of China (English)

    江先雨; 刘志伟; 叶飞娣


      目的分析2702例孕妇中期胎儿唐氏征筛查结果,探讨孕妇中期胎儿唐氏征产前筛查的临床意义.方法采用贝克曼库尔特全自动化学发光酶免分析仪及原装试剂,对孕14~20+6周的孕妇血清进行甲胎蛋白(AFP)、人绒毛膜促性激素β亚基(β-HCG)及游离雌三醇(uE3)检测,并将孕妇的相关信息和检测的 AFP、β-HCG、uE3结果输入“腾程筛查软件”中,进行三联检测的结果运算,得出一个概率指标.结果唐氏综合征(DS)筛查结果高危例数有65例,总阳性率为2.41%;其中:20~24岁年龄组的孕妇唐氏综合征筛查结果高危例数有11例(1.76%)25-29岁年龄组的孕妇唐氏综合征筛查结果高危例数有26例(1.93%);30-35岁年龄组的孕妇唐氏综合征筛查结果高危例数有9例(2.77%);>35岁年龄组的孕妇唐氏综合征筛查结果高危例数有19例(4.73%).结论在中期孕妇进行唐氏综合征三联筛查是必要的,能够提高唐氏患儿的早期发现和诊断,是降低唐氏患儿出生率的有效方法.%  Objective To discussed the middle for pregnant women fetal down the clinical significance of prenatal screening,2702 cases were analyzed for pregnant women fetus down the middle screening results. Methods Used the Beckman Kurt automatic chemiluminescence enzyme from analyzer and original reagent to detection of AFP, β-HCG and uE3 content in pregnant women of 14~20+6 weeks. Pregnant women's information and AFP, β-HCG and uE3 assessment input "Tengcheng software" for joint screening, get a probability index. Results Down syndrome (DS) screening high-risk number results 65 cases, the total prevalence rate was 2.41%; Among them: 20~24 years old age group of pregnant women down's syndrome screening results have high-risk number 11 cases(1.76%); 25~29 age group of pregnant women down's syndrome screening results high-risk number 26 cases (1.93%); 30~35 years old age group of pregnant women down

  1. Effect of anticancer therapy on Tn antigen exposure on the leucocyte membranes in patients with leukemia

    Directory of Open Access Journals (Sweden)

    G. S. Maslak


    Full Text Available Tn-antigen (Thomsen-nouvelle antigen is tumor-associated carbohydrate antigen with only one GalNAc residue attached to serine or threonine of polypeptide chain. There is not enough data about the expression of this glycotope in hematologic processes. But the correlations between increasing Tn-antigen expression on the cell surface and tumor growth progression, invasion, and activation of cell migration are well known. Therefore, the currently important area of modern research is studying of the impact of anticancer therapy by expression of this carbohydrate antigen in the onco-proliferative process. There are two types of cytostatic therapies in clinical hospitals of Ukraine: COP-therapy (cyclophosphamide, vincristine, prednisone and FC-therapy (fludarabine, cyclophosphamide, which are the most popular due to their effectiveness and low price. The aim of our study was to investigate Tn-antigen exposure on the surface of lymphocytes, monocytes and granulocytes in polycythemia vera and subleukemic myelosis; to examine the influence of COP- and FC-therapies on Tn-antigen exponation in patients with chronic lymphocytic leukemia. The objects of the study were blood cells of patients with chronic lymphocytic leukemia (n = 25, polycythemia vera (n = 15 and subleukemic myelosis (n = 15 aged 58–66 years. Healthy hematologic volunteers (n = 15 aged 55 to 65 years were in the control group. Lymphocytes of patients with chronic lymphocytic leukemia (n = 25 were also studied after the chemotherapy treatment of patients divided into two groups: those who took COP-therapy (n = 13; and those who treated with FC-therapy (n = 12. Tn-antigen exposure on lymphocytes, monocytes and granulocytes was investigated by Beckman Сoulter EPICS flow cytometer with primary monoclonal Tn-antigen anybodies (Institute of Immunology, Moscow, Russia and secondary fluorescein isothiocyanate labeled antybodies (Millipore, USA. The number of dead cells was monitored by binding

  2. Association of single nucleotide polymorphism at position 45 in adiponectin gene with plasma adiponectin level and insulin resistance in obesity

    International Nuclear Information System (INIS)

    Objective: To explore the association of single nucleotide polymorphism at position 45 (SNP45) in adiponectin gene with plasma adiponectin level and insulin resistance in obesity in Quanzhou area of Fujian province. Methods: Two hundred and forty-eight patients with obesity and 225 normal control subjects were enrolled in this study.Fasting insulin (FINS) were measured by radioimmunoassay and fasting plasma glucose (FPG), total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C) were measured by BECKMAN DXC800 biochemistry analyzer. Body mass index (BMI), waist to hip ratio,homeostasis model assessment of insulin resistance (HOMA-IR) were calculated. Plasma adiponectin levels were examined by means of enzyme-linked immunosorbentassy. The adiponectin gene SNP45 was identified by PCR-restriction fragment length polymorphism. Results: (1) Frequencies of GG+GT genotype in obesity group and normal control group were 61% and 44% respectively (χ2=14.182, P<0.01), and G allele frequencies were 35% and 25% (χ2=10.708, P<0.01). (2) In obesity group,the subjects with SNP45 GG+GT genotype had higher TG and LDL-C levels than those with TT genotype (t=2.604, P<0.01; t=5.507, P<0.01), and had lower adiponectin level than those with TT genotype (t=2.275, P<0.05), and had significantly lower HDL-L level than those with TT genotype (t=10.100, P< 0.01). (3) In normal control group,the subjects with SNP45 GG +GT genotype had significantly lower adiponectin,TG,TC levels than those with TT genotype (t=2.510, P<0.05; t=2.922, P<0.01; t=3.272, P< 0.01). (4) Logistic analysis proved that the SNP45 GG+GT genotype in obesity group was associated with decreased risk of plasma adiponectin level (OR=0.810, 95% CI : 0.673-0.975, P<0.05), and with increased risk of HOMA-IR (OR=1.746, 95% CI : 1.060-2.875, P<0.05). The SNP45 GG+GT genotype in normal control group was associated with increased risk of HOMA-IR (OR=3.962, 95

  3. Atividade elétrica cerebral do rato com lesões da formação reticular mesencefálica

    Directory of Open Access Journals (Sweden)

    Walter C. Pereira


    Full Text Available No presente estudo foram utilizados 73 ratos em preparações agudas e crônicas, nas quais lesamos a formação reticular mesencefálica com corrente contínua (3,5 a 4,0 mA durante 5 a 10 segundos. O eletródio ativo era implantado estereotàxicamente segundo as coordenadas de König e Klippel. As lesões eram feitas parcial ou totalmente, uni ou bilateralmente, e em todos os animais procedeu-se ao controle histológico das áreas lesadas, usando-se o método de Weil. O registro da atividade elétrica cortical foi feito com polígrafo Beckman de 4 canais, utilizando-se derivações bipolares curtas (1mm com eletródios esféricos de platina. As experiências permitiram as seguintes conclusões: 1 — As características eletrofisiológicas dos fusos que ocorrem após lesões da formação reticular mesencefálica são muito semelhantes às dos fusos espontâneos e barbitúricos, inclusive quanto à projeção cortical. Quanto à duração dos potenciais que os constituem, contudo, notamos que a faixa de variação era mais centuada (20 a 80 ms, o que pode ser atribuído à maior complexidade dos potenciais do cérebro isolado, possivelmente pela falta de ação cronadora da formação reticular sobre o sistema sincronizador talâmico. 2 — Os mecanismos envolvidos na gênese dos fusos do sono barbitúrico ou espontâneo e os do cérebro isolado são, pelo menos em parte, dependentes do bloqueio da formação reticular mesencefálica. 3 — A formação reticular mesencefálica ativa preferencialmente o hemisfério cerebral homolateral; o contingente cruzado talvez seja mobilizado somente quando estímulos alertantes intensos atingem o tegmento mesencefálico. 4 — Além da formação reticular mesencefálica deve haver outros mecanismos ativadores corticais, visto que, em preparações agudas de cérebro isolado, observamos: a surtos de curta duração de atividade dessincronizada; b oscilações freqüentes do ECoG durante o registro

  4. The Argonne Leadership Computing Facility 2010 annual report.

    Energy Technology Data Exchange (ETDEWEB)

    Drugan, C. (LCF)


    that will be faster than petascale-class computers by a factor of a thousand. Pete Beckman, who served as the ALCF's Director for the past few years, has been named director of the newly created Exascale Technology and Computing Institute (ETCi). The institute will focus on developing exascale computing to extend scientific discovery and solve critical science and engineering problems. Just as Pete's leadership propelled the ALCF to great success, we know that that ETCi will benefit immensely from his expertise and experience. Without question, the future of supercomputing is certainly in good hands. I would like to thank Pete for all his effort over the past two years, during which he oversaw the establishing of ALCF2, the deployment of the Magellan project, increases in utilization, availability, and number of projects using ALCF1. He managed the rapid growth of ALCF staff and made the facility what it is today. All the staff and users are better for Pete's efforts.

  5. "The human race is still a work in progress":An Interview with Richard Powers%"人类仍是一部进步中的作品":理查德·鲍尔斯访谈录

    Institute of Scientific and Technical Information of China (English)



    理查德·鲍尔斯是2006年美国国家图书奖小说奖得主,获奖作品《回声制造者》.他于1998年当选美国艺术与科学院院士,现任伊利诺伊大学厄巴纳-香槟分校英语系教授兼贝克曼高级研究院研究员.他屡获殊荣的另外8部小说包括:《我们歌唱的时代》、《冲破黑暗》、《赢利》、《葛拉蒂2.2》、《囚犯的困境》、《三个农民去舞会》等.这次访谈以作家的产生和小说的创作为主要内容,集中讨论了他的作家观和主要创作经历,同时也讨论了他在不同文化中的丰富经历及其对科学技术的广泛探索.在访谈中,他还就好小说给出了他的定义,阐释了作家主要关心的问题和特殊的创作视角,表达了他对文学批评的看法以及他对文学启迪人生意义的信念.本访谈是伊利诺伊大学东亚及太平洋研究中心弗里曼基金项目"创作视角与文学精神:当代美国作家研究"的部分成果.%Richard Powers is the 2006 winner of National Book Award for his fiction The Echo Maker. He was elected fellow of the American Academy of Arts and Sciences in 1998, and he is professor at the Department of English and a full-time member of the Beckman Institute, University of Illinois at Urbana-Champaign(UIUC). He is also author of eight other prize-winning novels:The Time of Our Singing, Plowing the Dark, Gain, Galatea 2.2, Operation Wandering Soul, The Gold Bug Variations, Prisoner's Dilemma and Three Farmers on Their Way to a Dance. This interview concentrates on questions about his idea of being a writer and the making of fiction in general, and also discusses his rich experience of different cultures and his extensive exploration in science and technology. Furthermore, Powers talks about the differences between writing fiction and pursuing science. He gives his definition of good fiction, and explains his main concerns as a writer, his vision of literary criticism, and his belief in

  6. Effect of propofol on glutamate-induced activation and elated inflammatory cytokines of astrocytes from spinal cord dorsal horn

    Institute of Scientific and Technical Information of China (English)

    Chengming Qin; Qing Li; Juying Liu; Tao Zhu; Yong Xiang


    BACKGROUND: Astrocytes participate in central nervous system-mediated physiological or pathological processes, such as pain. Activated dorsal horn astrocytes from the spinal cord produce nerve active substances and proinflammatory cytokines, such as interleukin-I beta (IL-1 β ), IL-6, and tumor necrosis factor-a (TNF-a ), which play important roles in pain transduction and regulation. OBJECTIVE: To investigate the effects of different doses of propofol on activation of cultured spinal cord dorsal horn astrocytes induced by glutamate, as well as changes in IL-1 β, IL-6, and TNF-a, and IL-10 (anti-inflammatory cytokine) expression in rats, and to explore the dose relationship of propofnl. DESIGN, TIME AND SETTING: The cellular and molecular biology experiment was performed at the Central Laboratory of Yunyang Medical College between March 2006 and December 2007. MATERIALS: Forty healthy, Wistar rats, aged 2-3 days, were selected. Propofol was provided by Zeneca, UK; glutamate by Sigma, USA; EPICS XL flow cytometry by Beckman culture, USA; rabbit-anti-mouse glial fibrillary acidic protein (GFAP) antibody kit and inflammatory cytokine detection kit were provided by Zhongshan Biotechnology Company Ltd., Beijing; multimedia color pathologic image analysis system was a product of Nikon, Japan. METHODS: Astrocytes were harvested from T11-L6spinal cord dorsal horn of Wistar rats and incubated for 3 weeks. The cells were divided into seven groups, according to various treatment conditions: control group was cells cultured in Hank's buffered saline solution; intralipid group was cells cultured in intralipid (0.2 mL/L); glutamate group was cells cultured with 100 μ mol/L glutamate; propofol group was cells cultured with 250 μ mol/L propofol; three glutamate plus propofol groups were cultured in 100 μ mol/L of glutamate, followed by 5, 25, and 250 μ mol/L of prnpofol 10 minutes later. MAIN OUTCOME MEASURES: GFAP-labeled astrocytes were analyzed using a multimedia

  7. 焦虑对体液免疫功能影响及其与HLA-DQB1等位基因的关系%Influences of anxiety on humoral immune functions and the correlations with HLA-DQB1

    Institute of Scientific and Technical Information of China (English)

    高昆; 刘辉


    目的:研究焦虑对体液免疫功能的影响及其与HLA-DQB1等位基因多态性的关系.方法:随机挑选某医院健康体检住院医生31名,选用状态-特质焦虑量表(STAI)来测量其焦虑状况,实验室检测IgG、IgA、IgM以及补体C3、C4水平,并利用PCR扩增各研究对象HLA-DQB1*02、*03、*04、*05和*06五个位点基因多态性.结果:状态焦虑 (Ta)和特质焦虑 (Tc)均同补体C3呈正相关,有统计学意义.HLA-DQB1*02位点阳性和阴性的个体状态焦虑的差异有统计学意义(P<0.05),而补体C3的差异不具有统计学意义(P>0.05).HLA-DQB1* 04位点阳性和阴性的个体状态焦虑和特质焦虑的差异都有统计学意义(P<0.05),而补体C3的差异不具有统计学意义(P<0.05).结论:焦虑可以引起某些体液免疫功能指标的改变并与HLA-DQB1等位基因表型有关.%Objective:To investigate the association between anxiety and the change of humoral immune functions and its correlation with HLA-DQB1 polymorphisms.Methods:Total 31 resident doctors were selected randomly and tested by State-Trait Anxiety Inventory(STAI).IgG,IgA,IgM,complement C3 and complement C4 were detected with BECKMAN array360 system;HLA-DQB1*02、*03、*04、*05 and*06 alleles were individually amplified by polymerase chain reaction(PCR)using exon2 group-specific primers.The correlation between immune function and HLA-DQB1 polgmorphisms were investigated.Results:Statistical analysis showed that there was positive correlation with State Anxiety (Ta) and complement C3,either Trait Anxiety (Tc) and complement C3.There was significant difference between HLA-DQB1*02 positive and negative in Ta (P<0.05),while no difference in complement C3(P>0.05).There was significant difference between HLA-DQB1*04 positive and negative in Ta and Tc(P<0.05),while no difference in complement C3(P>0.05).Conclusion:Anxiety could change some humoral immune functions and this is related with HLA-DQB1 polymorphism.

  8. Leukocyte differential count in peripheral blood "with" single tube/ten colors"flow cytometry%单管10色流式细胞术分类计数外周血白细胞

    Institute of Scientific and Technical Information of China (English)

    刘雪凯; 潘娜; 王建中; 邢莹; 屈晨雪; 普程伟; 伍平; 王卉; 刘抗援; 马兰


    目的 应用"单管10色"流式细胞术分类计数外周血白细胞,并探讨其临床应用价值.方法 通过多种单克隆抗体的选择和多重逻辑设门的研究,确定了10色流式细胞术与12种单克隆抗体组合的单管免洗外周血白细胞分类方法(简称"单管10色"方法).选取142份外周血标本,以血涂片显微镜法分类技术为参考方法,同时用Beckman Cotdter LH750全自动血细胞分析仪和"单管10色"方法分类计数白细胞,计算"单管10色"方法的临床诊断效率,并统计分析两种方法与显微镜法分类计数白细胞的相关性.以BD FACS Calibur流式细胞仪免疫分型结果为标准,计算"单管10色"流式细胞术检出原始细胞的诊断效率.结果 "单管10色"流式细胞术与显微镜法分类计数外周血白细胞除嗜碱粒细胞外均具有良好的相关性(r均>0.700,P均0.700,P<0.01) except for basophils.The correlations with neutrophilic granulocytes,lymphocytes,immature granulocytes and blasts were superior(r=0.972,0.951,0.801,0.912,respectively,P<0.01).When 1% was selected as the cut-off point for immature granulocytes determined by standard microscopic cytology,the sensitivity and the specificity of"single tube/10 colors"flow cytometry were 92%(57/62) and 79% (63/80),respectively.When 0.5% was selected as the cut-off point for blasts detected by standard microscopic cytology,the sensitivity and the specificity were 99% (67/68) and 92% (68/74).Using the immunophenotyping results from BD FACS Calibur as a standard,the sensitivity for detecting blasts by"single tube/10 colOrs"flow cytometry was 100% (40/40),the specificity was 91% (10/11),the positive predictive value was 98% (40/41),the negative predictive value was 100% (10/10) and the accuracy was 98% (50/51).Conclusions The"single tube/10 colors"flow cytometry has a excellent correlation with the standard microscopic cytology when applied on leukocyte differential count in peripheral blood.It may potentially

  9. Radioactivity measurement with a plastic scintillation vial

    International Nuclear Information System (INIS)

    caused by CO2. One of the methods without scintillation cocktail is using a solid scintillator (ReadyCap, Beckman). However, the method needs to dry up the sample, so it requires certain time to dry up and is not applicable for volatile compounds. Also the sample could not be recovered and the solid waste would be produced. Another method is the Cherenkov counting. The method is applicable for the nuclides emitting beta rays with energy more than 260 keV, if water is used for the medium of Cherenkov effect. To detect lower energies, Takiue et al. proposed a method using a high-refractive transparent ceramic. The method, however, needs to dry up the sample. In contrast, using the ScintiVial, one does not need to dry up the sample. More than ten times use of the ScintiVial may be less expensive than the whole c.ost of liquid scintillation counting, which includes 10-mL cocktail, counting vial, and the cost for incineration. Conclusion: The ScintiVial method to measure radioactivity using a liquid scintillation counter was developed. The counting efficiency for 32P was 28-10% and that for 125I was 3%. This method is quite simple, is less expensive, and do not produce any radioactive organic liquid waste. Furthermore the sample can be recovered.


    Institute of Scientific and Technical Information of China (English)

    薛淑群; 尹洪滨


    Metaphase chromosome specimens of Polyodon spathula, injected with PHA and colchicin, were prepared from short-term culture of kidney cells with air-drying technique. It diploid chromosome number was 2n = 120. The karyotype was composed of 44m, 32sm and 44st, t chromosomes, NF = 196. Diploid nucleus DNA content was measured from the somatic cell of P. spathula, using flow cytometer( Beckman cell Lab Quant, made in USA) and the DNA content (2.3pg/ N) of erythrocytes of chicken ( Gallus domestica) as standard. The DNA content of P. spathula was 6.18pg/N , which was corresponded with the result measured chromosome number as ploidy. According to the present results and date reported, P. spathula was possibly a tetraploid (4n). Combination of the present results and data reported among Acipenseriformes including two families and six genera, Acipenseriformes was the polyploid origin's fish, and was quite completely special at the cell level which evolved by chromosome doubling. The chromosome's doubling and differentiation possibly were primary caused of polyploidization and polyploid type (4n, 8n, 12n and so on) richly. Polyoidization had played an important role in the diversity of Acipenseriformes.%采用体内注射PHA和秋水仙素,肾细胞短期培养,常规空气干燥法制备匙吻鲟(Polyodon spathula)的染色体标本.对其肾细胞染色体数目统计分析表明,匙吻鲟染色体组南120条染色体所组成.以测得的核型参数和按Levan,et al.提出的染色体划分标准得出:具有22对中部着丝粒染色体(m),16对亚中部着丝粒染色体(sm),22对亚端部着丝粒染色体(st)和端部着丝粒染色体(t);染色体臂数(NF)为196,核型公式为44 m + 32am + 44st,t.再采用流式细胞仪分析系统测定匙吻鲟体细胞的DNA含量,与鸡血细胞标准对照相比为2.69±0.19,以鸡红细胞DNA含量2.3 pg/N测算.则匙吻鲟体细胞DNA含量为6.18 pg/N.根据所得到的结果并结合已发表的鲟鱼类DNA含量和

  11. Infrared Absorption by Atmospheric Aerosols in Mexico City during MILAGRO. (United States)

    Kelley, K. L.; Mangu, A.; Gaffney, J. S.; Marley, N. A.


    Past research in our group using cylindrical internal reflectance spectroscopy has indicated that aqueous aerosols could contribute to the radiative warming as greenhouse species (1,2). Although aerosol radiative effects have been known for sometime and are considered one of the major uncertainties in climate change modeling, most of the studies have focused on the forcing due to scattering and absorption of radiation in the uv- visible region (3). Infrared spectral information also allows the confirmation of key functional groups that are responsible for enhanced absorption observations from secondary organics in the uv-visible region. This work extends our efforts to evaluate the infrared absorption by aerosols, particularly organics, that are now found to be a major fraction of urban and regional aerosols in the 0.1 to 1.0 micron size range and to help identify key types of organics that can contribute to aerosol absorption. During the MILAGRO campaign, quartz filter samples were taken at 12-hour intervals from 5 am to 5 pm (day) and from 5 pm to 5 am (night) during the month of March 2006. These samples were taken at the two super-sites, T-0 (Instituto Mexicano de Petroleo in Mexico City) and T-1 (Universidad Technologica de Tecamac, State of Mexico). The samples have been characterized for total carbon content (stable isotope mass spectroscopy) and natural radionuclide tracers, as well as for their UV-visible spectroscopic properties by using integrating sphere diffuse reflectance spectroscopy (Beckman DU with a Labsphere accessory). These same samples have been characterized in the mid and near infrared spectral ranges using diffuse reflection spectroscopy (Nicolet 6700 FTIR with a Smart Collector accessory). Aerosol samples were removed from the surfaces of the aerosol filters by using Si-Carb sampler. The samples clearly indicate the presence of carbonyl organic constituents and the spectra are quite similar to those observed for humic and fulvic acids

  12. Enhanced UV Absorption in Carbonaceous Aerosols during MILAGRO and Identification of Potential Organic Contributors. (United States)

    Mangu, A.; Kelley, K. L.; Marchany-Rivera, A.; Kilaparty, S.; Gunawan, G.; Gaffney, J. S.; Marley, N. A.


    Measurements of aerosol absorption were obtained as part of the MAX-Mex component of the MILAGRO field campaign at site T0 (Instituto Mexicano de Petroleo in Mexico City) during the month of March, 2006 by using a 7- channel aethalometer (Thermo-Anderson). These measurements, obtained at 370, 470, 520, 590, 660, 880, and 950 nm at a 5 minute time resolution, showed an enhanced absorption in the UV over that expected from carbon soot alone. Samples of fine atmospheric aerosols (less than 0.1micron) were also collected at site T0 and T1 (Universidad Technologica de Tecamac, State of Mexico) from 5 am to 5 pm (day) and from 5 pm to 5 am (night) during the month of March 2006. The samples were collected on quartz fiber filters with high volume impactor samplers. The samples have been characterized for total carbon content (stable isotope ratio mass spectroscopy) and natural radionuclide tracers (210Pb, 210Po, 210Bi, 7Be, 13C, 14C, 40K, 15N). Continuous absorption spectra of these aerosol samples have been obtained in the laboratory from 280 to 900nm with the use of an integrating sphere coupled to a UV-visible spectrometer (Beckman DU with a Labsphere accessory). The integrating sphere allows the detector to collect and spatially integrate the total radiant flux reflected from the sample and therefore allows for the measurement of absorption on highly reflective or diffusely scattering samples (1). The continuous spectra also show an enhanced UV absorption over that expected from carbon soot and the general profiles are quite similar to those observed for humic and fulvic acids found as colloidal materials in surface and groundwaters (2), indicating the presence of humic-like substances (HULIS) in the fine aerosols. The spectra also show evidence of narrow band absorbers below 400 nm typical of polycyclic aromatics (PAH) and nitrated aromatic compounds. Spectra were also obtained on NIST standard diesel soot (SRM 2975), NIST standard air particulate matter (SRM 8785

  13. Expression of nerve growth factor mRNA in splenic lymphocytes of bronchial asthma rats and its influencing actors

    Institute of Scientific and Technical Information of China (English)

    Jihong Dai; Yonghong Wang; Haixia He


    BACKGROUND: Previous research has proved that nerve growth factor (NGF) participates in the onset of asthma by the induction of neurogenic inflammation.OBJECTIVE: To investigate the effect of interleukin-13 (IL-13) and interferon-γ(IFN-γ) on the expression of NGF mRNA in the splenic lymphocytes of bronchial asthma rats.DESIGN, TIME AND SETTING: The experiment, a completely randomized study based on cellular immunology, was performed in the Laboratory of Neurology in Chongqing Medical University and the Department of Clinical Pharmacy in College of Clinical Medicine, Chongqing Medical University (Chongqing, China) from January 2006 to April 2007.MATERIALS: Four adult male Wistar rats were used in this study. Rat IL-13, IFN-γprobe and the total RNA extraction kit were produced by Shanghai Sangon Biological Technology & Services Co., Ltd (China). The NGF ELISA kit was a product of Wuhan Boster Bioengineering Co., Ltd (China). A Du-70 automatic UV spectrophotometer was produced by Beckman Company (USA).METHODS: Rats were subjected to 1-mL intraperitoneal injections each containing 100 mg of ovalbumin, and were sensitized by using antigen solution, which was sensitized with 5×109 Bacillus pertussis and 100 mg aluminum hydroxide powder. Four rats were challenged with 1% ovalbumin using an ultrasonic nebulizer for 60 minutes to establish an asthmatic model. After rats were anesthetized, splenic lymphocytes were isolated and cultured in medium, which was supplemented with IL-13 or IFN-γ, for 0, 12, 24 or 48 hours. A parallel study was conducted with cultured splenic lymphocytes, which were divided into a control group, an IL-13 group and an IFN-γ group. Culture medium was added with different concentrations of IL-13 (10, 50, 100 μg/L) and IFN-γ (1, 10, 50 μg/L); 24 hours later, all samples were harvested.MAIN OUTCOME MEASURES: The expression levels of NGF mRNA were detected by reverse transcription-polymerase chain reaction.RESULTS: In the control group, the

  14. Progress toward curing HIV infection with hematopoietic cell transplantation

    Directory of Open Access Journals (Sweden)

    Petz LD


    Full Text Available Lawrence D Petz,1 John C Burnett,2 Haitang Li,3 Shirley Li,3 Richard Tonai,1 Milena Bakalinskaya,4 Elizabeth J Shpall,5 Sue Armitage,6 Joanne Kurtzberg,7 Donna M Regan,8 Pamela Clark,9 Sergio Querol,10 Jonathan A Gutman,11 Stephen R Spellman,12 Loren Gragert,13 John J Rossi2 1StemCyte International Cord Blood Center, Baldwin Park, CA, USA; 2Department of Molecular and Cellular Biology, Irell and Manella Graduate School of Biological Sciences, 3Department of Molecular and Cellular Biology, Beckman Research Institute, City of Hope, Duarte, CA, USA; 4CCR5-Δ32/Δ32 Research Department, StemCyte International Cord Blood Center, Baldwin Park, CA, USA; 5Department of Stem Cell Transplantation, University of Texas MD Anderson Cancer Center, Houston, TX, USA; 6MD Anderson Cord Blood Bank, Department of Stem Cell Transplantation, University of Texas MD Anderson Cancer Center, Houston, TX, USA; 7Carolinas Cord Blood Bank, Duke University Medical Center, Durham, NC, USA; 8St Louis Cord Blood Bank, SSM Cardinal Glennon Children's Medical Center, St Louis, MO, USA; 9Enhance Quality Consulting Inc., Oviedo, FL, USA; 10Cell Therapy Service and Cord Blood Bank, Banc de Sang i Teixits, Barcelona, Spain; 11BMT/Hematologic Malignancies, University of Colorado, Aurora, CO, USA; 12Immunobiology and Observational Research, CIBMTR, Minneapolis, MN, USA; 13National Marrow Donor Program/Be The Match, Minneapolis, MN, USA Abstract: HIV-1 infection afflicts more than 35 million people worldwide, according to 2014 estimates from the World Health Organization. For those individuals who have access to antiretroviral therapy, these drugs can effectively suppress, but not cure, HIV-1 infection. Indeed, the only documented case for an HIV/AIDS cure was a patient with HIV-1 and acute myeloid leukemia who received allogeneic hematopoietic cell transplantation (HCT from a graft that carried the HIV-resistant CCR5-Δ32/Δ32 mutation. Other attempts to establish a cure for HIV

  15. Analysis of changes in the percentage of B (CD19) and T (CD3) lymphocytes, nk cells, subsets CD4, CD8 in differentiated thyroid cancer patients treated with iodine-131

    International Nuclear Information System (INIS)

    Objective: To evaluate the changes in the percentage of B (CD19) and T (CD3) lymphocytes, NK cells, subsets CD4, CD8 in patients with differentiated thyroid carcinoma (DTC) who received iodine-131 for therapeutic purposes. Methods: In this study, 102 DTC patients were divided into three groups. Group A, 8 cases received 1850 MBq of iodine-131 for the remnant thyroid ablation. Group B, 43 cases received 3700 MBq of iodine-131 for the treatment of cervical lymph node metastasis. Group C, 51 cases received 7400 MBq of iodine-131 for remote metastasis. All patients were in a hypothyroid state at the time of administration of iodine-131 and resumed L-thyroxine (2μg/Kg/day) 5 days after iodine-131 administration. The percentage of B and T lymphocytes, NK cells, subsets CD4, CD8 in peripheral blood were serially analyzed at baseline and at days 7, 30 and 90 after iodine-131 administration using a Coulter EPICS XL cytometer. Ten healthy individuals were used as a control group for lymphocyte subset values. Results: Comparing the basal lymphocyte subset levels in groups A, B and C with the control group, only NK cells showed significantly higher levels in patients than in controls (P=0.043). In group A, only the percentage of NK cells (P=0.031) and B cells (P =0.024) were reduced at day 7. In group B, a decrease in the percentage of NK cells at days 7(P=0.005), 30 (P=0.021) was observed, while a significant decrease in the percentage of B cells was only observed at day 7(P=0.006). Among T cells, only CD4+ was obviously affected, resulting in a reduction in the CD4+/CD8+ ratio at day 30 (P=0.034). In group C, patients showed a decrease in the percentage of NK cells at days 7 (P=0.023), 30 (P=0.006). A decrease in the percentage of both B and T lymphocytes was observed at days 7(P=0.020, 0.018 respectively), 30(P=0.041, 0.025 respectively). Among T cells, a decrease in the percentage of CD4+ and an increase in the percentage of CD8+ were observed, resulting in a marked

  16. P-gp Expression and Rh 123 Efflux Assay Have no Impact on Survival in Egyptian Pediatric Acute Lymphoblastic Leukemia Patients

    International Nuclear Information System (INIS)

    In a previous work we have studied MDR status in terms of P-glycoprotein (P-gp) expression and Rhodamine 123 efflux assay in Egyptian acute leukemia patients. We have reported results comparable to the literature as regards ANLL both in pediatric and adult cases. However, higher figures were encountered for the functional assay in ALL. As our ALL cases especially in pediatric age group show worse prognosis compared to literature, we hypothesized that the higher percentage of cases with positive Rh 123 efflux assay might be a contributing factor. Material and Methods: A total of 108 cases were studied including 80 ALL and 28 ANLL. ALL cases included 48 male and 32 female with an age range of 6m to 18 yrs and a median of 7 yrs. ANLL cases included 18 male and 10 female with an age range of 6m to 18 yrs and a median of 8 yrs. P-gp expression was evaluated using 4E3 and DIC2 mAb, analyzed by Coulter XL flow cytometer and expressed as a ratio at a cut off of ≤1.1 and/or ≤5% positive cells. For the evaluation of MDR function Rh 123 efflux assay using cyclosporine as a blocker and expressed as a ratio at a cutoff of ≤1. 1 and/or ≤ 10% positive cells was performed. MDR expression and function were correlated to age, Hb, TLC, CD34 expression, immuno phenotype and DNA index in ALL, FAB subtypes in ANLL as well as to CR, DFS and EFS in ALL. In ALL, P-gp expression was encountered in 26.4% of cases. Positive Rh efflux was reported in 61.5%. No correlation was encountered between neither expression nor functional assay with age, Hb, TLC, CD34 expression or immuno phenotype. CR was achieved in 89.8%; neither P-gp expression nor Rh123 efflux had an impact on CR except for Rh 123 efflux in T-ALL where a cutoff of 1.25 could predict CR at a total accuracy of 70.6%. DFS was 92.3% while EFS was 72.2% for the whole group. No significant difference was encountered neither between cases expressing or lacking P-gp nor between cases with negative or positive Rh 123 efflux assay

  17. 生长激素对胰腺癌IGF-Ⅰ,Ⅱ-IGFBP3通路动态影响的实验研究%Effect of growth hormone on features of IGF-Ⅰ-Ⅱ-IGFBP3 pathway in pancreatic cancer

    Institute of Scientific and Technical Information of China (English)

    石毅; 孙跃明; 白剑锋; 陆文熊; 傅赞; 赵翰林; 苗毅


    Objective To investigate the effect of GH on proliferation of pancreatic cancer cells and observe the features of IGF-IGFBP3 pathway in the host after GH administration. Methods Pancreatic cancer cells (SW-1990,PANC-1 and P3) during exponential growth stage were harvested and cultured in medium containing growth hormone (50 ng/ml). After 24, 48 and 72 hours, cells were counted using a Coulter Counter. Thirty-five Athymic nude Balb/c mice were inoculated with SW-1990cells. When tumors became palpable after inoculation, animals were randomized to receive GH points (1 h, 2 h, 6 h, 24 after the last injection), plasma samples were gathered for subsequent ELISA determination and liver was rapidly incised for immune blotting analysis. Results The results revealed that GH stimulated cell growth in vitro. GH elevated levels of IGF-Ⅰ , Ⅱ at the 1st , 2nd , 6th hour after the last injection. GH augmented the expression of IGFBP3 in the liver of the host in vivo (1 h, 2 h, 6 h, 24 h, respectively). Conclusion Such proteins as IGF- Ⅰ and Ⅱ might be associated with mechanism of last effect of GH on tumor host. The up-regulation of IGFBP3 by GH administration in the host may help to explain the phenomena that GH doesn't accelerate growth of pancreatic tumor in vivo.%目的 观察生长激素(GH)对胰腺癌细胞增殖及对肿瘤宿主胰岛素样生长因子Ⅰ,Ⅱ(IGF-Ⅰ,Ⅱ)-胰岛素样生长因子结合蛋白3(IGFBP3)通路的影响,初步探讨体内外实验差异的原因.方法 体外应用GH(50 ng/ml)24 h,48 h,72 h后计数胰腺癌细胞SW-1990,PANC-1及P3;选用BALB/C雌性裸小鼠35只,以SW-1990成瘤,测量瘤体积,成瘤裸鼠随机分为注射GH的实验组(GH组)及对照组(NS组),荷瘤裸鼠在最后依次注射GH后第1小时、2小时、6小时及24小时处死动物,心室穿刺抽血,切除肝脏,酶联免疫吸附法(ELISA)检测血清IGF-Ⅰ,-Ⅱ水平(P<0.05),蛋白印记(Western Blot)方法观察肝脏IGFBP3的

  18. Desempenho de uma semeadora-adubadora direta, em função da velocidade de eslocamento e do tipo de manejo da biomassa da cultura de cobertura do solo Performance of a seeding-fertilizer machine in function of the displacement speed and soil cover crop biomass management

    Directory of Open Access Journals (Sweden)

    Klinger B. Branquinho


    Full Text Available O objetivo do presente trabalho foi estudar o desempenho de uma semeadora-adubadora, operando em duas velocidades sobre a palhada da cultura do milheto (Pennisetum glaucum (L. R. Brown, após diferentes manejos, na semeadura direta da cultura da soja (Glycine Max L.. O experimento foi realizado com seis tratamentos, em esquema fatorial, delineados em blocos ao acaso, com quatro repetições. Os tratamentos combinaram três manejos (triturador de palhas tratorizado, rolo- faca e herbicida com duas velocidades de deslocamento da semeadora-adubadora (5,2 e 7,3 km h-1. Foram analisadas as seguintes variáveis: acúmulo e decomposição de massa seca do milheto, índice de emergência da cultura da soja, uniformidade de distribuição longitudinal de sementes, capacidade de campo efetiva da semeadora-adubadora e produtividade da soja. Os resultados mostraram que não houve diferença na decomposição da massa de milheto após os manejos e que esses, juntamente com a velocidade de deslocamento do conjunto trator-semeadora-adubadora, não influenciaram no número de dias para a emergência das plântulas de soja e na distribuição longitudinal das sementes. A capacidade de campo efetiva da semeadora-adubadora foi maior na velocidade mais alta. O rendimento de grãos não diferiu significativamente nos tratamentos estudados.The objective of the present work was to evaluate three different managements of the Pennisetum glaucum (L. R. Brown crop and two-speed displacement in a no-tillage system of soybean (Glycine max L. crop on a typical Eutrophic Red Latosol. The experiment was arranged in six treatments, in a factorial array, in a randomized complete block design, with four replications. The treatments were constituted from three combination of crop managements (straw chopper, residue coulter and herbicide and two speed displacement of the seeding-fertilizer machine (5.2 and 7.3 km h-1. The following variables have been analyzed: emergency index of

  19. Preparation of in-house dextran and its clinical applications in sentinel node studies, Siriraj Hospital

    International Nuclear Information System (INIS)

    From 2003, an in-house Dextran for sentinel node study has been prepared at Division of Nuclear Medicine, Siriraj Hospital. The technique is the 'Pramongkutklao modified method'. Main materials: Dextran powder (M.W. 70000, Sigma), Stannous chloride dihydrate crystals (Sn2Cl2.2H2O, Sigma), water-for-injection (WFI), HCl, and NaOH. Procedure: Dextran was dissolved in WFI. Sn2Cl2.2H2O, 4% in HCl was added to the Dextran solution of which the pH was subsequently adjusted to 6.5-7.0 using NaOH. The final volume was reached via WFI and the solution was passed through a 0.22 μ Millipore-filter into N2-purged vials, 0.5 ml in each aliquot. They were then kept in a freezer (-20 deg. C) for up to 6 months. For each lot, the bacteriological and radiochemical purity were checked at the Department of Microbiology and at the Division of Nuclear Medicine, respectively. The bacteriological testing showed no growth after two weeks of incubation. The radiochemical purity study was performed using 2 systems - paper chromatography/ Acetone, and ITLC-SG/ Methyl-Ethyl- Ketone (MEK) with 8-cm strips giving the radiochemical purity of approximately 95% or above, as shown. However, the first system is routinely used because it is much cheaper. The stability of the labelled compound or bench-life was done three times showing the radiochemical purity of above 95% (paper/ Acetone) through out 6-7 h after labelling. Periodically, the particle size of Dextran in the kit was checked using the Sub-Micron Particle Analyser Coulter Model N4 MD at the Faculty of Pharmacology, Mahidol University. It came out to be around 100 nm. For clinical applications in Siriraj Hospital, at the moment 99mTc-Dextran is requested by two surgeons to use in two main groups of the patients - those with breast cancer and those with skin cancer (either malignant melanoma or squamous cell carcinoma). For breast cancer, two categories are used: non-imaging and imaging. The former is done to localize a small tumour using

  20. ZAP-70 as A Possible Prognostic Factor in Childhood Acute Lymphoblastic Leukemia

    International Nuclear Information System (INIS)

    Background: Zeta-chain-associated protein (ZAP- 70) is a 70kD adaptor protein that acts quickly after T cell activation to propagate signal. The role of ZAP-70 in Tcell function is well established, and in the previous years, this molecule was considered to be T-cell specific. More recent data have documented a role of ZAP-70 in B cells. Interest in ZAP-70 has grown since it has been shown, through gene expression profiling, that it is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL). Purpose: The aim of this study was to investigate the expression of ZAP-70 in leukemic blasts of 50 newly diagnosed patients of B-lineage acute lymphoblastic leukemia (ALL), and to assess the correlation between ZAP-70 expression and various prognostic factors and outcome. Patients and Methods: This study included 50 pediatric patients with newly diagnosed B-lineage ALL. They were 28 males (56%) and 22 females (44%) presented to the Pediatric Oncology Department, National Cancer Institute, Cairo University, during the period from 2005 to 2007. The age range was 2 to 17 years with a mean of 8.58±5.8 years and median 8 years. All patients were subjected at presentation to a full clinical history and physical examination. Patients diagnosed with ALL were enrolled on St. Jude Total XV protocol: standard risk and low risk according to results of primary investigation. Immuno phenotyping was done using monoclonal antibodies which were analyzed on Coulter XL (Panel included CD1, CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD19, CD22, Cytoplasmic m, anti k, anti l, CD13, CD33, anti classII MHC and TdT). Cases were considered ZAP-70 positive when exhibiting a ZAP/GAPDH (Glyceraldehyde- 3-phosphate dehydrogenase) ratio ³0.13. Results: The study revealed expression of ZAP-70 in 5/50 cases (10%). There was no statistically significant relation between ZAP-70 expression and the following: age, Total Leukocytic Count, hepatomegaly and splenomegaly. There

  1. The role of versican G3 domain in regulating breast cancer cell motility including effects on osteoblast cell growth and differentiation in vitro – evaluation towards understanding breast cancer cell bone metastasis

    Directory of Open Access Journals (Sweden)

    Du William


    Full Text Available Abstract Background Versican is detected in the interstitial tissues at the invasive margins of breast carcinoma, is predictive of relapse, and negatively impacts overall survival rates. The versican G3 domain is important in breast cancer cell growth, migration and bone metastasis. However, mechanistic studies evaluating versican G3 enhanced breast cancer bone metastasis are limited. Methods A versican G3 construct was exogenously expressed in the 66c14 and the MC3T3-E1 cell line. Cells were observed through light microscopy and viability analyzed by Coulter Counter or determined with colorimetric proliferation assays. The Annexin V-FITC apoptosis detection kit was used to detect apoptotic activity. Modified Chemotactic Boyden chamber migration invasion assays were applied to observe tumor migration and invasion to bone stromal cells and MC3T3-E1 cells. Alkaline phosphatase (ALP staining and ALP ELISA assays were performed to observe ALP activity in MC3T3-E1 cells. Results In the four mouse breast cancer cell lines 67NR, 66c14, 4T07, and 4T1, 4T1 cells expressed higher levels of versican, and showed higher migration and invasion ability to MC3T3-E1 cells and primary bone stromal cells. 4T1 conditioned medium (CM inhibited MC3T3-E1 cell growth, and even lead to apoptosis. Only 4T1 CM prevented MC3T3-E1 cell differentiation, noted by inhibition of alkaline phosphatase (ALP activity. We exogenously expressed a versican G3 construct in a cell line that expresses low versican levels (66c14, and observed that the G3-expressing 66c14 cells showed enhanced cell migration and invasion to bone stromal and MC3T3-E1 cells. This observation was prevented by selective EGFR inhibitor AG1478, selective MEK inhibitor PD 98059, and selective AKT inhibitor Triciribine, but not by selective JNK inhibitor SP 600125. Versican G3 enhanced breast cancer cell invasion to bone stromal cells or osteoblast cells appears to occur through enhancing EGFR/ERK or AKT signaling

  2. Parâmetros solo-máquina em função de doses de resíduos vegetais e profundidades de deposição de adubo em semeadura direta Parameters of soil-machine in function of the amount of cover residues and different fertilizer deposition depths in direct drilling

    Directory of Open Access Journals (Sweden)

    Karina M. Kamimura


    Full Text Available O tráfego contínuo e inadequado de máquinas em solos sob semeadura direta tem provocado alterações dos atributos físicos e mecânicos dos solos, influenciando, dessa forma, na produtividade das culturas. O experimento foi conduzido na Estação Experimental Agronômica da Universidade Federal do Rio Grande do Sul, no município de Eldorado do Sul - RS, num Argissolo Vermelho distrófico típico, com o objetivo de quantificar a força de tração e obter informações sobre os atributos físicos do solo, em semeadura direta sobre resíduos de aveia-preta e ervilhaca parcialmente decomposta. O delineamento experimental utilizado foi em blocos casualizados, com parcelas subsubdivididas, sendo, nas parcelas principais, doses de resíduos da cultura de inverno (0; 1,3; 2,6; 3,2; 3,8 e 5,1 Mg ha-1 de palha de aveia-preta consorciada com ervilhaca, nas subparcelas profundidades de atuação da haste sulcadora de adubo (0,06 e 0,12 m e nas subsubparcelas tráfegos dos rodados do trator e colhedora. Os atributos físicos do solo foram afetados pelo tráfego dos rodados da máquina. A força de tração foi influenciada pela profundidade de atuação das hastes sulcadoras e o tráfego dos rodados.The continuous and inadequate machine traffic on soils under direct drilling has been changing the soil physical and mechanical properties, influencing therefore crop productivity. The experiment was carried out at the Agricultural Research Station of the Universidade Federal do Rio Grande do Sul (Eldorado do Sul, RS, Brazil on Typic Paleudlt. The main aim of the work was to measure coulter draft requirements and physical attributes under black oats (Avena strigosa Schieb and common vetch (CV (Vicia sativa L. partially decomposed. The experimental design was carried out in randomized blocks, with split-split-plots and three repetitions. The main treatments were plots with different amount of residues of the winter crop (0, 1,3; 2,6; 3,2; 3,8 and 5,1 Mg ha-1

  3. High efficiency CIP 10-I personal inhalable aerosol sampler

    International Nuclear Information System (INIS)

    particles being deposited on the lower horizontal plate. At the beginning of the vertical tubing, the mutually opposing particle trajectories limit particle wall deposition by virtual impaction effect. The inner selector walls are polished to avoid particles being stopped by eventual surface asperities. Particle size-dependent sampling efficiency was measured in the laboratory wind tunnel. The experimental aerosol was composed of polydisperse glass micro-spheres. The size analysis of the particles collected was done by the Coulter Counter technique. The transmission efficiency (reciprocal to wall losses) was found to be close to 100 % for the entire range of particle sizes, and indicated no particle loss. The overall sampling efficiency was measured using a rotating bluff body at an external wind speed of 1 m/s. The rotating bluff body represents a scaled torso of an operator. The 'high efficiency' CIP 10-I (I for inhalable) responds fairly well to the conventional CEN-ISO-ACGIH criteria for sampling the inhalable health-related aerosol fraction.

  4. 高温烟气颗粒物在线检测装置性能评价%Performance assessment of high temperature flue gas on-line particle size analyzer

    Institute of Scientific and Technical Information of China (English)

    许乔奇; 姬忠礼; 杨亮


    In order to evaluate the performance of on-line particles test equipment at high temperature, an optical on-line particle size analyzer was used to test catalyst particles concentration and particle size distribution (PSD) downstream of a fluid catalytic cracking (FCC) flue gas filtration test facility. At the same time, an off-line sampling system tested catalyst particles concentration both upstream and downstream of the facility. Catalyst PSD and microstructure were measured with Coulter counter particle size analyzer and scanning electron microscopy (SEM). The off-line test results agreed well with the results of on-line particle size analyzer. Experimental results showed that the on-line test equipment had high accuracy when operating pressure was 0. 21 Mpa and operating temperature ranged from 320°C to 465°C. Experimental and simulation results showed that operating temperature almost had no influence on test measurement. On this basis, aiming at real condition of coal chemical industry, an iterated method was developed to correct on-line measurement results, which could provide a guideline to determine the particulate matter in high temperature pipeline in coal chemical industry.%为评价高温工况下颗粒物在线检测装置的性能,在催化裂化高温烟气过滤实验装置上使用光学在线颗粒物检测装置测定了过滤器下游烟气中的催化剂浓度及粒径分布,同时用离线式粉尘等动采样装置和Coulter粒径分析仪测最过滤器上游与下游的催化剂浓度和粒径分布,对在线式的测量结果进行验证.考虑了温度对在线式测量结果的影响.实验结果表明:在线式检测装置可以在操作温度320~465℃,操作压力0.21 MPa工况下实现稳定的测最,在线式测量结果和离线式测量结果吻合很好,实验和计算模拟结果表明操作温度对实验测量的影响可以忽略.在此基础上,针对煤化工行业的实际工况,提出了利用迭代方式来获得

  5. The influence of melanin on bystander effect

    International Nuclear Information System (INIS)

    The influence of melanin on bystander effect has been studied. Melanin is known to be a natural substance with proved radioprotective properties. It is effective only against low dose radiation. The observed result in colony-forming activity of HPV-G cells may be due to the potential radioprotective effect of melanin. HPV-G cells (human keratinocyte line, immortalised by transfection with the HVP virus) are cultured in Dulbecco's MEM: F12 (1:1) Medium. In addition, 50 ml of Fetal Calf Serum, 5 ml penicillin-streptomycin, 5 ml L-glutamine and 1 g/ml of hydrocortisone were added to medium. Cells were maintained in an incubator at 37 degrees Celsius, with 95% humidity and 5% Carbon Dioxide and routinely subcultured every 8-10 days. Melanin was isolated from animal hair (Belarus Pharmaceutical Association, Minsk). Melanin was added to cell medium at 1 mg/l concentration. Cell suspension after dilution was counted using a Coulter counter. Appropriate cell numbers were plated according to the Puck and Marcus technique in 5 ml medium in 25cm2 NUNC flasks. There were 3 types of flasks: direct irradiation, bystander donor and bystander recipient. Each of 3 them had 4 triplet sets: control, melanin, irradiated cells 0,5 Gy and irradiated cells 0,5 Gy with melanin added. Melanin is added into flasks 30 min - 1 hour before irradiation. Bystander donor and direct flasks are irradiated and replaced back in the incubator at 37 degree centigrade for one hour. Culture flasks are irradiated at room temperature using a Co 60 teletherapy source, delivering approximately 2 Gy per minute at a source-to cell distance of 80 cm. 1 hour after irradiation the medium from bystander donor flasks is removed and filtered through NALGENE 0,2 m sterile syringe filter to ensure that no cells are present in the medium. This filtered medium is used to replace the medium from bystander recipient flasks. All flasks are left for 9-10 days (until colonies are visible) and then stained with Carbol Fusion

  6. Changes of biochemical indexes in patients with acute cerebral infarction after treatment with simvastatin

    Institute of Scientific and Technical Information of China (English)

    Cheng Yang


    BACKGROUND: At present, it is believed that the important causes of cerebral infarction are the disorders of lipid metabolism and endothelial function, and the outcomes of clinical treatment can be improved by regulating serum lipids and antiinflammation, etc.OBJECTIVE: To observe the effect of simvastatin, inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, on the levels of serum lipids, serum enzymic indexes and inflammatory metabolic indexes in patients with cerebral infarction.DESIGN : A comparative observation.SETTING: Department of Geriatrics, Longquanshan Hospital of Liuzhou City.PARTICIPANTS: Forty-eight patients with acute cerebral infarction wera selected from the Department of Geriatrics of Longquanshan Hospital of Liuzhou from March 2004 to February 2006, including 24 males and 24females, the mean age was (54±12) years, average disease course was (10.0±4.5) days. They were all accorded with the diagnostic standard for cerebral infarction set by the Fourth National Academic Meeting for Cerebrovascular Disease in 1999, and cerebral hemorrhage was excluded by cranial CT scanning. The 48patients were randomly divided into control group (n =24) and traatment group (n =24). Informed consents were obtained from all the participants.METHODS: ① All the patients wera treated according to the symptoms, besides those in the traatment group were given simvastatin (Harbin Pharm. Group Sanjing Pharmaceutical Shareholding, Co.,Ltd., No. H20010454;Batch number: 20040218; 5 mg/tablet). The initial dosage was 10 mg per day for 4 weeks, and then increased to 30 mg per day for another 4 weeks. ② Before treatment and within 1 week after treatment, the total cholesterol, triglyceride, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST), craatine kinase and C raactive protein in serum were determined with Beckman-cx7 automatic biochemical analytical apparatus in both groups. ③ The

  7. A special year, thanks and a look ahead (United States)

    Birch, David J. S.


    Dear authors, reviewers and readers of Measurement Science and Technology , I would like to thank all those who have published papers with us in 2013, and offer a special thanks to those of you who have given up much of your precious time and kindly reviewed articles for the journal. 2013 was of course a very special year for the journal, marking the 90th anniversary of the journal's foundation, as Journal of Scientific Instruments. In order to mark the anniversary we started the year with a historical perspective of the journal [1], and a historical review of 90 years of dielectric properties measurement of materials [2]. The celebrations continued with a stimulating meeting at the Institute of Physics in London in March, entitled 'Frontiers of Measurement'. In keeping with the multidisciplinary ethos of the journal, the speakers covered a diverse range of topics, all linked by the common language of measurement science. Patrick Gill of NPL talked about atomic clocks, Bruce Tromberg of the Beckman Laser Institute and Medical Clinic covered biophotonics, Roger Bisby of the University of Salford lectured on developments in biochemical techniques, Ivan Marusic of the University of Melbourne spoke on fluids and Mervyn Miles of the University of Bristol described recent developments in atomic force microscopy. Nicola Gulley, Editorial Director of IOP Publishing (IOPP), briefed the meeting on some of the latest developments in the world of scientific publishing. The meeting ended with a presentation to Sharon D'Souza, outgoing Publisher of the journal, and a welcome for Ian Forbes who has taken over the role. On behalf of all the Editorial Board I would like to offer a very special thanks to Sharon for all her great work for the journal over many years and wish her every success in her new IOPP posts as Publisher of Reports in Progress in Physics and the recently launched Methods and Applications in Fluorescence. Ian Forbes comes to the journal with a PhD in Physics

  8. In vitro release of 1,3-bis (2-chloroethyl)-1-nitrosourea sustained-release microspheres and the distribution in rat brain tissues

    Institute of Scientific and Technical Information of China (English)

    Xia Li; Liping Guo; Qin Li


    BACKGROUND: The implantation of released chemotherapeutic drugs, which takes biodegradable polymer as vector, into the tumor site can get high concentration and release the drug for a long time, it can directly act on the tumor cells, and reduce the general toxicity.OBJECTIVE: To explore the in vitro and in vivo course of 1,3-bis (2-chloroethyl)-1-nitrosourea (BCNU) sustained-release from BCNU-loaded polylactide (PLA) microspheres (MS) and location in rat brain tissue.DESIGN: A repetitive measurement.SETTING: Central Pharmacy, General Hospital of Chinese People's Armed Police Forces.MATERIALS: Thirty male SD rats were used. PLA (Mr5000, batch number: KSL8377) was produced by Wako Pure Chemical Inc.,Ltd. (Japan); BCNU (batch number: 021121) by Tianjin Jinyao Amino Acid Co., Ltd.;BCNU-PLA-MS was prepared by the method of solvent evaporation and pressed into tablets (10 mg/tablet).High-performance liquid chromatography (HPLC) Agilent 1100 (USA); LS9800 liquid-scintillation radiometric apparatus (Beckman). Chromatographic conditions: Elite Hypersil ODS2 C18 chromatographic column (5 μm,4.6 mm ×150 mm); Mobile phase: methanol: water (50:50), flow rate was 1.0 mL per minute, wave length of ultraviolet detection was 237 nm, and the inlet amount of samples was 10 μL.METHODS: The experiments were carried out in the experimental animal center of the General Hospital of Chinese Armed Police from May 2004 to July 2005. ① In vitro BCNU-PLA-MS release test: BCNU-PLA-MS was prepared by the method of solvent evaporation, then placed in 0.1 mol/L phosphate buffered solution (PBS, pH 7.4, 37 ℃), part of MS were taken out at 1, 2, 3, 7, 10 and 15 days respectively, and the rest amount of BCNU in MS was determined by HPLC, then the curve of BCNU-PLA-MS release was drawn. ②In vivo BCNU-PLA-MS release and distribution test: The rats were anesthetized, then BCNU-PLA-MS were implanted to the site 1 mm inferior to the cortex of frontal lobe. Five rats were killed postoperatively

  9. Editorial - [FSRJ]Future Studies Research Journal - V7N1

    Directory of Open Access Journals (Sweden)

    Renata Giovinazzo Spers


    artigo que foi aprovado no Fast Track do III SINGEP. A análise desta pesquisa pode auxiliar na construção do planejamento estratégico de uma pequena empresa em fase de crescimento para que seus objetivos estratégicos sejam atingidos.“Aplicação da Criatividade para Gerar Projetos de Inovação: O Caso Prático de uma Estratégia Didática” também foi aprovado no Fast Track III SINGEP. Os autores: Eliana Maria dos Santos Pereira Alves e Ovídio Felippe Pereira da Silva Júnior discutem elementos como identificação de oportunidades, criatividade, inovação e empreendedorismo, buscando sua inter-relação para compreender como é possível motivar a geração de propostas de projetos inovadores em ambientes de ensino.Os autores Mariana Bassi Sutter, Laura Ferranty Mac Lennan, Gabriela Pelegrini Tiscoski e Edison Fernandes Polo, abordam o tema de brasilidade com o objetivo de compreender as representações da imagem do Brasil e os diversos significados do conceito de brasilidade por diferentes pontos de vista.Competitividade Agressiva é o tema abordado pelo autor Antônio Benedito Oliveira Junior e especificamente, demostra a importância da CA para a sobrevivência e crescimento da empresa em mercados cada vez mais dinâmicos, turbulentos e competitivos.O artigo “A Influência do Nível de Competências e de Maturidade em Gestão de Projetos no Resultado Corporativo de uma Empresa do Setor de Transformação” de autoria de Gislaine Cristina dos Santos Teixeira, Emerson Antônio Maccari e Daniel Simonsen, tem por objetivo analisar a influência das competências e da maturidade em gestão de projetos das equipes matriciais e dos gerentes no desempenho dos projetos e consequentemente no resultado corporativo. Boa leitura! Renata Giovinazzo SpersEditora Científica James Terence Coulter WrightEditor Internacional 

  10. The effect of reaction parameters on microwave-induced combustion synthesis of Al2O3-TiC powders

    International Nuclear Information System (INIS)

    SiC/Al2O3 cement susceptor, which then was enclosed in a refractory brick. The samples were ignited in a 2.45 GHz Ratheon Radarline multimode microwave oven. An inconel-shield K-type thermocouple and a two color infrared pyrometer were used to monitor the temperature. The X-ray diffraction, ultrapycnometer, BET, Coulter method, Scanning electron microscopy, and Energy dispersive X-ray spectroscopy were used to characterize the synthesized powders. In this study, the Plackett-Burman was first performed to analyze the significance of reaction parameters on the ignition behavior and characteristics of the resulting powders. The reaction parameters studied were the amount of excess Al and Al2O3, particle size of each reactant, heating method, and microwave power. Once important factors were identified, more extensive investigation involving only these dominant factors was conducted using the CCD. ANOVA was used to provide a quantitative measure of confidence of the data. The combustion synthesis of Al2O3-TiC powders using MH and MHH was successfully achieved. The most significant factors affecting the investigated responses were amount of excess Al and Al2O3, particle size of C and Al2O3, and also heating method. The 30-run CCD was then performed. A clear difference in ignition time and temperature was observed for the two heating methods. Approximately 7 sec to 1.50 min. were required for the MH ignited samples to reach the ignition temperatures while MHH ignited samples required longer times, 20 sec to 6 min. The MHH samples ignited at higher temperature than MH samples and resulted in lower combustion temperature. The results showed no significant difference hi the characteristics of powders ignited by these two heating methods. The densities of powders were in the range of 3.4-4.20 g/cm3. The specific surface area and the particle sizes of the ignited powders were 0.8-3.8 m2/g and 0.5-2.1 microns, respectively. The addition of diluents to the reactants increased

  11. Preparo do solo em áreas de produção de grãos, silagem e pastejo: efeito na resistência tênsil e friabilidade de agregados Soil tillage in grain and silage-producing areas and pasture: effect on tensile strength and aggregate friability

    Directory of Open Access Journals (Sweden)

    Marina Araújo Bavoso


    ção da F. Os resultados demonstraram que a RT foi influenciada pelos sistemas de produção e tipos de preparo do solo. O menor valor de RT foi verificado no solo sob Pastejo, enquanto o Sistema Plantio Direto apresentou o maior valor de RT. A friabilidade não distinguiu os sistemas de produção e tipos de preparo de solo estudados.The understanding and quantification of soil use and management on the soil physical quality are important to develop and select sustainable agricultural systems. The purpose of this study was to evaluate the structural quality of a clayey Brazilian Oxisol under different production and soil tillage systems by measuring the tensile strength (TS and to quantify aggregate friability (F. The study was conducted in Castro, Paraná state, in southern Brazil, using three production systems and seven soil tillage types, in a randomized block design. The following production systems were evaluated: (PS I - ryegrass for soil cover (winter and corn for grain production (summer; (PS II ryegrass for haylage/silage and corn for grain production; and (PS III - ryegrass for grazing and corn for grain production. Seven types of soil tillage were evaluated in each system: (G1 - plow harrow in winter, depth 0.15 m; (G2 - plow harrow in winter and summer, depth 0.15 m; (Plow - disk plow, depth 0.20 m; (Laser wing subsoiler - subsoiler wing tips, to a depth of 0.45 m; (Subsoiler - subsoiler, depth 0.80 m; (Aerator - soil aerater Aeromix®; and (NT - No-tillage, where ryegrass was sown using a double disc and corn with a coulter. From each experimental plot soil blocks (0.20 x 0.15 x 0.07 m were taken which were broken up by hand in their natural aggregates. Then, the aggregates were air-dried for 24 h and passed through sieves with diameters of 12.5 and 19 mm. Forty aggregates from each block were selected and tested for indirect tension to determine TS and quantify F. TS was influenced by the production systems and soil tillage types. The lowest and the

  12. Editorial - [FSRJ]Future Studies Research Journal - V6N2

    Directory of Open Access Journals (Sweden)

    Renata Giovinazzo Spers


    éticos do país a partir da maior utilização de gases combustíveis para o aquecimento residencial de água. Aprovado no Fast Track do Semead 2014, o artigo “Quantificação dos planos estratégicos por meio do orçamento empresarial: uma aplicação prática com métodos estocásticos” os autores Marino Luiz Eyerkaufer, Janaina Poffo Possamai, Mirian Buss Conçalves, apresentam neste estudo uma aplicação de métodos estocásticos em que o probabilismo se demonstra como alternativa de minimização das incertezas quanto à assertividade das estimativas. Boa leitura!James Terence Coulter WrightEditorRenata Giovinazzo SpersEditora Adjunta

  13. Phase 1 Final Technical Report - MgB2 Synthesis: Pushing to High Field Performance

    International Nuclear Information System (INIS)

    crystalline boron results in the formation of parasitic phases such as MgB4, MgB7, etc. Such parasitic phases are a primary element of the connectivity problem, in which even though a sample powder may contain grains of high-quality MgB2, adjacent grains are surrounded by intergrowths of parasitic phases so that current trans-port is badly degraded. The best results to date have been obtained using boron powder produced long ago for a rocket propellant development project. The synthesis process was complex and is now largely lost, and the manufacturing equipment has long since been scrapped. The last batch of the powder has been used during recent years to support MgB2 R and D at several labs, but supplies are dwindling. ATC has identified a first application of its plasma torch to synthesize phase-pure amorphous boron flake using a rapid-quench splat technique. Inexpensive technical-grade boron would be purified of contaminants, then dispersed as an aerosol in inert gas and passed through the plasma torch to melt it into a spray. The spray would be splat-condensed on a rotating drum to form pure amorphous flake. The process would begin with technical-grade boron powder, having good stoichiometric purity, nanoscale particles, but significant contamination of MgO and crystalline boron. We used wet chemistry to remove B2O3 completely and reduced the MgO impurity, and analyzed the particle size distribution using a Coulter counter and the phase composition using X-ray diffrac-tion (XRD). The next step will be to build an rf plasma torch with a recirculating single-component aerosol feed and the cooled splat drum and collector, and undertake process devel-opment for amorphous boron powder. This revised goal has two benefits. First, it is an easier technology than our ultimate goal of a multi-component laminar flow torch. We have been counseled by those experienced in plasma torch technology that our ultimate goal will require a torch that should be feasible but has never been

  14. Preface: SciDAC 2009 (United States)

    Simon, Horst


    and posters goes to the teams of researchers, the success of this year's conference is due to the strong efforts and support from members of the 2009 SciDAC Program Committee and Organizing Committee, and I would like to extend my heartfelt thanks to them for helping to make the 2009 meeting the largest and most successful to date. Program Committee members were: David Bader, LLNL; Pete Beckman, ANL; John Bell, LBNL; John Boisseau, University of Texas; Paul Bonoli, MIT; Hank Childs, LBNL; Bill Collins, LBNL; Jim Davenport, BNL; David Dean, ORNL; Thom Dunning, NCSA; Peg Folta, LLNL; Glenn Hammond, PNNL; Maciej Haranczyk, LBNL; Robert Harrison, ORNL; Paul Hovland, ANL; Paul Kent, ORNL; Aram Kevorkian, SPAWAR; David Keyes, Columbia University; Kwok Ko, SLAC; Felice Lightstone, LLNL; Bob Lucas, ISI/USC; Paul Mackenzie, Fermilab; Tony Mezzacappa, ORNL; John Negele, MIT; Jeff Nichols, ORNL; Mike Norman, UCSD; Joe Oefelein, SNL; Jeanie Osburn, NRL; Peter Ostroumov, ANL; Valerio Pascucci, University of Utah; Ruth Pordes, Fermilab; Rob Ross, ANL; Nagiza Samatova, ORNL; Martin Savage, University of Washington; Tim Scheibe, PNNL; Ed Seidel, NSF; Arie Shoshani, LBNL; Rick Stevens, ANL; Bob Sugar, UCSB; Bill Tang, PPPL; Bob Wilhelmson, NCSA; Kathy Yelick, NERSC/LBNL; Dave Zachmann, Vista Computational Technology LLC. Organizing Committee members were: Communications: Jon Bashor, LBNL. Contracts/Logistics: Mary Spada and Cheryl Zidel, ANL. Posters: David Bailey, LBNL. Proceedings: John Hules, LBNL. Proceedings Database Developer: Beth Cerny Patino, ANL. Program Committee Liaison/Conference Web Site: Yeen Mankin, LBNL. Tutorials: David Skinner, NERSC/LBNL. Visualization Night: Hank Childs, LBNL; Valerio Pascucci, Chems Touati, Nathan Galli, and Erik Jorgensen, University of Utah. Again, my thanks to all. Horst Simon San Diego, California June 18, 2009

  15. Without 'Focus'

    Directory of Open Access Journals (Sweden)

    Aldo Sevi


    Full Text Available It is widely accepted that a notion of 'focus', more or less as conceived of in Jackendoff (1972, must be incorporated into our theory of grammar, as a means of accounting for certain observed correlations between prosodic facts and semantic/pragmatic facts. In this paper, we put forth the somewhat radical idea that the time has come to give up this customary view, and eliminate 'focus' from our theory of grammar. We argue that such a move is both economical and fruitful.Research over the years has revealed that the correlations between prosody, 'focus', and the alleged semantic/pragmatic effects of focus are much less clear and systematic than we may have initially hoped. First we argue that this state of affairs detracts significantly from the utility of our notion of 'focus', to the point of calling into question the very motivation for including it in the grammar. Then we look at some of the central data, and show how they might be analyzed without recourse to a notion of 'focus'. We concentrate on (i the effect of pitch accent placement on discourse congruence, and (ii the choice of 'associate' for the so-called 'focus sensitive' adverb only. We argue that our focus-free approach to the data improves empirical coverage, and begins to reveal patterns that have previously been obscured by preconceptions about 'focus'.ReferencesBeaver, D. & Clark, B. 2008. Sense and Sensitivity: How Focus Determines Meaning. Blackwell.Beaver, D., Clark, B., Flemming, E., Jaeger, T. F. & Wolters, M. 2007. ‘When semantics meets phonetics: Acoustical studies of second occurrence focus’. Language 83.2: 245–76., M. & Hirschberg, J. 1994. ‘The ToBI Annotation Conventions’. Ms.,, D. 1972. ‘Accent is predictable (if you are a mind-reader’. Language 48.3: 633–44.üring, D. 2006. ‘Focus projection and default

  16. Preface: SciDAC 2008 (United States)

    Stevens, Rick


    Microsoft Research at a new conference facility in Redmond, Washington. Over 90 people attended the tutorials, which covered topics ranging from an introduction to BG/P programming to advanced numerical libraries. The SciDAC and INCITE programs and the DOE Office of Advanced Scientific Computing Research core program investments in applied mathematics, computer science, and computational and networking facilities provide a nearly optimum framework for advancing computational science for DOE's Office of Science. At a broader level this framework also is benefiting the entire American scientific enterprise. As we look forward, it is clear that computational approaches will play an increasingly significant role in addressing challenging problems in basic science, energy, and environmental research. It takes many people to organize and support the SciDAC conference, and I would like to thank as many of them as possible. The backbone of the conference is the technical program; and the task of selecting, vetting, and recruiting speakers is the job of the organizing committee. I thank the members of this committee for all the hard work and the many tens of conference calls that enabled a wonderful program to be assembled. This year the following people served on the organizing committee: Jim Ahrens, LANL; David Bader, LLNL; Bryan Barnett, Microsoft; Peter Beckman, ANL; Vincent Chan, GA; Jackie Chen, SNL; Lori Diachin, LLNL; Dan Fay, Microsoft; Ian Foster, ANL; Mark Gordon, Ames; Mohammad Khaleel, PNNL; David Keyes, Columbia University; Bob Lucas, University of Southern California; Tony Mezzacappa, ORNL; Jeff Nichols, ORNL; David Nowak, ANL; Michael Papka, ANL; Thomas Schultess, ORNL; Horst Simon, LBNL; David Skinner, LBNL; Panagiotis Spentzouris, Fermilab; Bob Sugar, UCSB; and Kathy Yelick, LBNL. I owe a special thanks to Mike Papka and Jim Ahrens for handling the electronic theater. I also thank all those who submitted videos. It was a highly successful experiment. Behind the

  17. Browning boreal forests of western North America (United States)

    Verbyla, David


    's boreal forest Remote Sens. 2 2729-47 Riordan B, Verbyla D and McGuire A D 2006 Shrinking ponds in subarctic Alaska based on 1950-2002 remotely sensed images J. Geophys. Res. 111 G04002 Ruess R W, McFarland J M, Trummer L M and Rohrs-Richey J K 2009 Disease-mediated declines in N-fixation inputs by Alnus tenuifolia to early-successional floodplains in interior and south-central Alaska Ecosystems 12 489-502 Stafford J M, Wendler G and Curtis J 2000 Temperature and precipitation of Alaska: 50 year trend analysis Theor. Appl. Climatology 67 33-44 Stow D, Peterson A, Hope A, Engstrom R and Coulter L 2007 Greenness trends of Arctic tundra vegetation in the 1990s: comparison of two NDVI data sets from NOAA AVHRR systems Int. J. Remote Sens. 28 4807-22 van Mantgem P J et al 2009 Widespread increase of tree mortality rates in the western United States Science 323 521-4 Walsh, J E, Chapman W L, Romanovsky V, Christensen J H and Stendel M 2008 Global climate model performance over Alaska and Greenland J. Clim. 21 6156-74 Wendler G and Shulski M 2009 A century of climate change for Fairbanks, Alaska Arctic 62 295-300 Zhang K, Kimball J S, Hogg E H, Zhao M, Oechel W C, Cassano J J and Running S W 2008 Satellite-based model detection of recent climate-driven changes in northern high-latitude vegetation productivity J. Geophys. Res. 113 G03033

  18. only: A Case Study In Projective Meaning

    Directory of Open Access Journals (Sweden)

    Craige Roberts


    Full Text Available I offer an integrated theory of the meaning of only in which the prejacent, while not presupposed, is both entailed and backgrounded, hence tends to project (following a general proposal about projection due to Simons et al. 2010. Moreover, I argue, contra Beaver & Brady (2008, that only is not conventionally associated with focus, the focus effects arising instead pragmatically. But I do adopt aspects of their semantics for only, including the presupposition of a pre-order over the elements of its domain.ReferencesAbbott, B. 2000. ‘Presuppositions as non-assertions’. Journal of Pragmatics 32: 1419–37., B. 2008. ‘Presuppositions and common ground’. Linguistics and Philosophy 31: 523–538., P. 2010. ‘Entailment, assertion, and textual coherence: the case of almost and barely’. Linguistics 48, no. 3: 525–545., P., Roberts, C. & Allyn Smith, E. 2008. ‘Review of The Logic of Conventional Implicature by Chris Potts’. Linguistics and Philosophy 30: 707–749., J. D. 1993. ‘The importance of being “only”: testing the neo-Gricean versus neoentailment paradigms’. Journal of Semantics 10: 301–18., D. 2001. Presupposition and Assertion in Dynamic Semantics. CSLI Publications, Stanford, CA.Beaver, D. 2010. ‘Handout from a talk on projective meaning’. Stuttgart, Germany.Beaver, D. & Brady, C. 2003. ‘Always and Only: Why not all focus sensitive operators are alike’. Natural Language Semantics 11: 323–62., D. & Brady, C. 2008. Sense and Sensitivity. Blackwell., M. E. & Ayers, G. M. 1994. ‘Guidelines for ToBI labeling guide, ver. 2.0’. On-line at The Ohio State University: www