Sample records for bk010044 beckman coulter

  1. CLSI-based transference of CALIPER pediatric reference intervals to Beckman Coulter AU biochemical assays. (United States)

    Abou El Hassan, Mohamed; Stoianov, Alexandra; Araújo, Petra A T; Sadeghieh, Tara; Chan, Man Khun; Chen, Yunqi; Randell, Edward; Nieuwesteeg, Michelle; Adeli, Khosrow


    The CALIPER program has established a comprehensive database of pediatric reference intervals using largely the Abbott ARCHITECT biochemical assays. To expand clinical application of CALIPER reference standards, the present study is aimed at transferring CALIPER reference intervals from the Abbott ARCHITECT to Beckman Coulter AU assays. Transference of CALIPER reference intervals was performed based on the CLSI guidelines C28-A3 and EP9-A2. The new reference intervals were directly verified using up to 100 reference samples from the healthy CALIPER cohort. We found a strong correlation between Abbott ARCHITECT and Beckman Coulter AU biochemical assays, allowing the transference of the vast majority (94%; 30 out of 32 assays) of CALIPER reference intervals previously established using Abbott assays. Transferred reference intervals were, in general, similar to previously published CALIPER reference intervals, with some exceptions. Most of the transferred reference intervals were sex-specific and were verified using healthy reference samples from the CALIPER biobank based on CLSI criteria. It is important to note that the comparisons performed between the Abbott and Beckman Coulter assays make no assumptions as to assay accuracy or which system is more correct/accurate. The majority of CALIPER reference intervals were transferrable to Beckman Coulter AU assays, allowing the establishment of a new database of pediatric reference intervals. This further expands the utility of the CALIPER database to clinical laboratories using the AU assays; however, each laboratory should validate these intervals for their analytical platform and local population as recommended by the CLSI. Copyright © 2015 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  2. Evaluation of Beckman Coulter DxI 800 immunoassay system using clinically oriented performance goals. (United States)

    Akbas, Neval; Schryver, Patricia G; Algeciras-Schimnich, Alicia; Baumann, Nikola A; Block, Darci R; Budd, Jeffrey R; Gaston, S J Stephen; Klee, George G


    We evaluated the analytical performance of 24 immunoassays using the Beckman Coulter DxI 800 immunoassay systems at Mayo Clinic, Rochester, MN for trueness, precision, detection limits, linearity, and consistency (across instruments and reagent lots). Clinically oriented performance goals were defined using the following methods: trueness-published desirable accuracy limits, precision-published desirable biologic variation; detection limits - 0.1 percentile of patient test values, linearity - 50% of total error, and consistency-percentage test values crossing key decision points. Local data were collected for precision, linearity, and consistency. Data were provided by Beckman Coulter, Inc. for trueness and detection limits. All evaluated assays except total thyroxine were within the proposed goals for trueness. Most of the assays met the proposed goals for precision (86% of intra-assay results and 75% of inter-assay results). Five assays had more than 15% of the test results below the minimum detection limits. Carcinoembryonic antigen, total thyroxine and free triiodothyronine exceeded the proposed goals of ±6.3%, ±5% and ±5.7% for dilution linearity. All evaluated assays were within the proposed goals for instrument consistency. Lot-to-lot consistency results for cortisol, ferritin and total thyroxine exceeded the proposed goals of 3.3%, 11.4% and 7% at one medical decision level, while vitamin B12 exceeded the proposed goals of 5.2% and 3.8% at two decision levels. The Beckman Coulter DxI 800 immunoassay system meets most of these proposed goals, even though these clinically focused performance goals represent relatively stringent limits. Copyright © 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  3. A critical evaluation of the Beckman Coulter Access hsTnI: Analytical performance, reference interval and concordance. (United States)

    Pretorius, Carel J; Tate, Jillian R; Wilgen, Urs; Cullen, Louise; Ungerer, Jacobus P J


    We investigated the analytical performance, outlier rate, carryover and reference interval of the Beckman Coulter Access hsTnI in detail and compared it with historical and other commercial assays. We compared the imprecision, detection capability, analytical sensitivity, outlier rate and carryover against two previous Access AccuTnI assay versions. We established the reference interval with stored samples from a previous study and compared the concordances and variances with the Access AccuTnI+3 as well as with two commercial assays. The Access hsTnI had excellent analytical sensitivity with the calibration slope 5.6 times steeper than the Access AccuTnI+3. The detection capability was markedly improved with the SD of the blank 0.18-0.20 ng/L, LoB 0.29-0.33 ng/L and LoD 0.58-0.69 ng/L. All the reference interval samples had a result above the LoB value. At a mean concentration of 2.83 ng/L the SD was 0.28 ng/L (CV 9.8%). Carryover (0.005%) and outlier (0.046%) rates were similar to the Access AccuTnI+3. The combined male and female 99th percentile reference interval was 18.2 ng/L (90% CI 13.2-21.1 ng/L). Concordance amongst the assays was poor with only 16.7%, 19.6% and 15.2% of samples identified by all 4 assays as above the 99th, 97.5th and 95th percentiles. Analytical imprecision was a minor contributor to the observed variances between assays. The Beckman Coulter Access hsTnI assay has excellent analytical sensitivity and precision characteristics close to zero. This allows cTnI measurement in all healthy individuals and the capability to identify numerically small differences between serial samples as statistically significant. Concordance in healthy individuals remains poor amongst assays. Crown Copyright © 2018. Published by Elsevier Inc. All rights reserved.

  4. [Evaluation of the analyzer of hematology Beckman Coulter® HmX™ in the university hospital of Oran]. (United States)

    Zmouli, N; Moulasserdoun, K; Seghier, F


    The choice of an automaton of haematology is a determining stage, which has to take into account at the same time the quality of the results and the economic imperatives: workload, structure and organization of the laboratory. [corrected] It is in this spirit that we estimated during a period of 3 months the analyzer of haematology: the HmX™ Coulter with boatman of samples of the company Beckman. This automaton realizes the blood numeration, the formula leukocytic and the reticulocyte count. At first, we estimated the appropriate characteristics of device. Secondly, we estimated the relevance, the sensibility and the specificity of the alarms by comparing with the reference method, which is the optical microscopy. For that purpose, 125 blood smears resulting from service of haematology and from resuscitation were examined in optical microscopy. The technical tests were realized according to the recommendations of the International committee for evaluation of automatons of haematology. The analytical performances were satisfactory in particular the big interval of linearity and the absence of contamination. As regards the evaluation of the alarms system: rate of rejection is 63%, the sensibility 86%, the specificity 70%, the positive predictive value 80%, the negative predictive value 78% and the efficiency 80%. The alarms myelaemia and atypical lymphocytes were never sources of false negatives. The alarms erythroblasts and platelet aggregates did not engendered positive forgery. The blast cell alarm was responsible for a single case of false negative. The faithfulness of automaton is satisfactory: the absence of contamination, the big interval of linearity for the leukocytes, the red blood cells and the platelets as well as a good relevance of the alarms with regard to the anomalies found on the peripheral blood smear. From the user-friendliness and practicability point of view, the HmX™ Coulter was deeply appreciated. Copyright © 2013 Elsevier Masson SAS. All

  5. A Comparison of Mindray BC-6800, Sysmex XN-2000, and Beckman Coulter LH750 Automated Hematology Analyzers: A Pediatric Study. (United States)

    Ciepiela, Olga; Kotuła, Iwona; Kierat, Szymon; Sieczkowska, Sandra; Podsiadłowska, Anna; Jenczelewska, Anna; Księżarczyk, Karolina; Demkow, Urszula


    Modern automated laboratory hematology analyzers allow the measurement of over 30 different hematological parameters useful in the diagnostic and clinical interpretation of patient symptoms. They use different methods to measure the same parameters. Thus, a comparison of complete blood count made by Mindray BC-6800, Sysmex XN-2000 and Beckman Coulter LH750 was performed. A comparison of results obtained by automated analysis of 807 anticoagulated blood samples from children and 125 manual microscopic differentiations were performed. This comparative study included white blood cell count, red blood cell count, and erythrocyte indices, as well as platelet count. The present study showed a poor level of agreement between white blood cell enumeration and differentiation of the three automated hematology analyzers under comparison. A very good agreement was found when comparing manual blood smear and automated granulocytes, monocytes, and lymphocytes differentiation. Red blood cell evaluation showed better agreement than white blood cells between the studied analyzers. To conclude, studied instruments did not ensure satisfactory interchangeability and did not facilitate a substitution of one analyzer by another. © 2016 Wiley Periodicals, Inc.

  6. The effect of sodium bicarbonate and validation of beckman coulter AU680 analyzers for measuring total carbon dioxide (TCO2) concentrations in horse serum. (United States)

    Dirikolu, Levent; Waller, Pamela; Waguespack, Mona Landry; Andrews, Frank Michael; Keowen, Michael Layne; Gaunt, Stephen David


    This study evaluated the usage of Beckman Coulter AU680 analyzers for measurement of TCO 2 in horse serum, and the effect of sodium bicarbonate administrations on serum TCO 2 levels in resting horses. Treatment of horses with sodium bicarbonate did not result in any adverse events. Mean TCO 2 concentration was significantly higher from 1 to 8 h in the sodium bicarbonate-treated horses compared to the untreated controls. Within an hour, administration of sodium bicarbonate increased the TCO 2 level from 31.5 ± -2.5 (SD) to 34.0 ± 2.65 (SD) mmol/L and at 2-8 h post-administration, the TCO 2 level was above the 36 mmol/L cut-off level. In all quality control analysis of Australian standard by Beckman Coulter AU680 analyzer, the instrument slightly over estimated the TCO 2 level but the values were in close agreement with mean TCO 2 level being 38.03 with ± 0.87 mmol/L (SD). Expanded uncertainty was calculated using different levels of confidence interval. Based on 99.5% confidence interval using 0.805% expanded uncertainty using mean measured concentration of 38.05 mmol/L, it was estimated that any race samples TCO 2 level higher than 38.5 mmol/L will be indicative of sodium bicarbonate administration using Beckman Coulter AU680 analyzer in Louisiana.

  7. Precision, accuracy, cross reactivity and comparability of serum indices measurement on Abbott Architect c8000, Beckman Coulter AU5800 and Roche Cobas 6000 c501 clinical chemistry analyzers. (United States)

    Nikolac Gabaj, Nora; Miler, Marijana; Vrtarić, Alen; Hemar, Marina; Filipi, Petra; Kocijančić, Marija; Šupak Smolčić, Vesna; Ćelap, Ivana; Šimundić, Ana-Maria


    The aim of our study was to perform verification of serum indices on three clinical chemistry platforms. This study was done on three analyzers: Abbott Architect c8000, Beckman Coulter AU5800 (BC) and Roche Cobas 6000 c501. The following analytical specifications were verified: precision (two patient samples), accuracy (sample with the highest concentration of interferent was serially diluted and measured values compared to theoretical values), comparability (120 patients samples) and cross reactivity (samples with increasing concentrations of interferent were divided in two aliquots and remaining interferents were added in each aliquot. Measurements were done before and after adding interferents). Best results for precision were obtained for the H index (0.72%-2.08%). Accuracy for the H index was acceptable for Cobas and BC, while on Architect, deviations in the high concentration range were observed (y=0.02 [0.01-0.07]+1.07 [1.06-1.08]x). All three analyzers showed acceptable results in evaluating accuracy of L index and unacceptable results for I index. The H index was comparable between BC and both, Architect (Cohen's κ [95% CI]=0.795 [0.692-0.898]) and Roche (Cohen's κ [95% CI]=0.825 [0.729-0.922]), while Roche and Architect were not comparable. The I index was not comparable between all analyzer combinations, while the L index was only comparable between Abbott and BC. Cross reactivity analysis mostly showed that serum indices measurement is affected when a combination of interferences is present. There is heterogeneity between analyzers in the hemolysis, icteria, lipemia (HIL) quality performance. Verification of serum indices in routine work is necessary to establish analytical specifications.

  8. Pediatric reference value distributions and covariate-stratified reference intervals for 29 endocrine and special chemistry biomarkers on the Beckman Coulter Immunoassay Systems: a CALIPER study of healthy community children. (United States)

    Karbasy, Kimiya; Lin, Danny C C; Stoianov, Alexandra; Chan, Man Khun; Bevilacqua, Victoria; Chen, Yunqi; Adeli, Khosrow


    The CALIPER program is a national research initiative aimed at closing the gaps in pediatric reference intervals. CALIPER previously reported reference intervals for endocrine and special chemistry markers on Abbott immunoassays. We now report new pediatric reference intervals for immunoassays on the Beckman Coulter Immunoassay Systems and assess platform-specific differences in reference values. A total of 711 healthy children and adolescents from birth to reference intervals calculated in accordance with Clinical and Laboratory Standards Institute (CLSI) EP28-A3c guidelines. Complex profiles were observed for all 29 analytes, necessitating unique age and/or sex-specific partitions. Overall, changes in analyte concentrations observed over the course of development were similar to trends previously reported, and are consistent with biochemical and physiological changes that occur during childhood. Marked differences were observed for some assays including progesterone, luteinizing hormone and follicle-stimulating hormone where reference intervals were higher than those reported on Abbott immunoassays and parathyroid hormone where intervals were lower. This study highlights the importance of determining reference intervals specific for each analytical platform. The CALIPER Pediatric Reference Interval database will enable accurate diagnosis and laboratory assessment of children monitored by Beckman Coulter Immunoassay Systems in health care institutions worldwide. These reference intervals must however be validated by individual labs for the local pediatric population as recommended by CLSI.

  9. Comparison of five automated hematology analyzers in a university hospital setting: Abbott Cell-Dyn Sapphire, Beckman Coulter DxH 800, Siemens Advia 2120i, Sysmex XE-5000, and Sysmex XN-2000. (United States)

    Bruegel, Mathias; Nagel, Dorothea; Funk, Manuela; Fuhrmann, Petra; Zander, Johannes; Teupser, Daniel


    Various types of automated hematology analyzers are used in clinical laboratories. Here, we performed a side-by-side comparison of five current top of the range routine hematology analyzers in the setting of a university hospital central laboratory. Complete blood counts (CBC), differentials, reticulocyte and nucleated red blood cell (NRBC) counts of 349 patient samples, randomly taken out of routine diagnostics, were analyzed with Cell-Dyn Sapphire (Abbott), DxH 800 (Beckman Coulter), Advia 2120i (Siemens), XE-5000 and XN-2000 (Sysmex). Inter-instrument comparison of CBCs including reticulocyte and NRBC counts and investigation of flagging quality in relation to microscopy were performed with the complete set of samples. Inter-instrument comparison of five-part differential was performed using samples without atypical cells in blood smear (n=292). Automated five-part differentials and NRBCs were additionally compared with microscopy. The five analyzers showed a good concordance for basic blood count parameters. Correlations between instruments were less well for reticulocyte counts, NRBCs, and differentials. The poorest concordance for NRBCs with microscopy was observed for Advia 2120i (Kendall's τb=0.37). The highest flagging sensitivity for blasts was observed for XN-2000 (97% compared to 65%-76% for other analyzers), whereas overall specificity was comparable between different instruments. To the best of our knowledge, this is the most comprehensive side-by-side comparison of five current top of the range routine hematology analyzers. Variable analyzer quality and parameter specific limitations must be considered in defining laboratory algorithms in clinical practice.

  10. Comparison of Thyroglobulin Measurements Using Three Different Immunoassay Kits: A BRAMHS Tg-Plus RIA Kit, a BRAMHS hTg Sensitive Kryptor Kit, and a Beckman Coulter ACCESS Immunoassay Kit

    Directory of Open Access Journals (Sweden)

    Mijin Kim


    Full Text Available BackgroundSecond-generation thyroglobulin immunometric assays (Tg-IMAs have been developed with improved sensitivity. Our aim was to compare the diagnostic value of Tg-IMA measurements using a Kryptor (BRAHMS AG kit (Tg-K and an ACCESS (Beckman Coulter kit (Tg-A with that of the first-generation Tg measurement using a Tg-plus (BRAHMS AG kit (Tg+.MethodsWe enrolled 82 differentiated thyroid cancer patients who underwent total thyroidectomy with radioactive iodine remnant ablation and who underwent diagnostic whole body scan using recombinant human thyroid stimulating hormone (rhTSH. The Tg+, Tg-K, and Tg-A were measured before rhTSH administration during levothyroxine treatment (suppressed Tg from the same sample. Serum Tg+ was measured after rhTSH stimulation (stimulated Tg.ResultsSuppressed Tg+ was more significantly correlated with suppressed Tg-K (R2=0.919, P<0.001 than with suppressed Tg-A (R2=0.536, P<0.001. The optimal cut-off values of suppressed Tg+, Tg-K, and Tg-A for predicting stimulated Tg+ of 1 ng/mL were 0.3, 0.2, and 0.2 ng/mL, respectively. The sensitivity, specificity, and accuracy of suppressed Tg+ were 67%, 100%, and 90%, respectively; those of suppressed Tg-K were 83%, 90%, and 88%; those of suppressed Tg-A were 96%, 82%, and 87%, respectively. The positive predictive and negative predictive values of Tg+ were 100% and 87%, respectively; those of Tg-K were 79% and 92%; and those of Tg-A were 73% and 98%.ConclusionWe could not clearly demonstrate which kit had better diagnostic performance after comparison of first-generation Tg measurements with Tg-IMA measurements. Also, there were kit-to-kit variations between Tg-IMA kits. Suppressed Tg measured by Tg-IMA was insufficient to completely substitute for a stimulated Tg measurement.

  11. Microchip Coulter particle counter

    DEFF Research Database (Denmark)

    Larsen, Ulrik Darling; Blankenstein, Gert; Branebjerg, J.


    This paper presents a micro device employing the Coulter principle for counting and sizing of living cells and particles in liquid suspension. The microchip Coulter particle counter (μCPC) has been employed in a planar silicon structure covered with glass, which enables detailed observation during...

  12. Seed drill instrumentation for spatial coulter depth measurements

    DEFF Research Database (Denmark)

    Kirkegaard Nielsen, Søren; Munkholm, Lars Juhl; Lamandé, Mathieu


    coulter depth varied up to ±5 mm between the blocks. In addition, significant depth variations between the individual coulters were found. The mean depths varied between −14.2 and −25.9 mm for the eleven coulters. The mean shallowest coulter depth (−14.2 mm) was measured for the coulter running...... in the wheel track of the tractor. The power spectral densities (distribution) of the coulter depth oscillation frequencies showed that the majority of oscillations occurred below 0.5 Hz without any natural vibration frequency. The study concluded that the instrumentation concept was functional for on...

  13. Platelet counting using the Coulter electronic counter. (United States)

    Eggleton, M J; Sharp, A A


    A method for counting platelets in dilutions of platelet-rich plasm using the Coulter electronic counter is described.(1) The results obtained show that such platelet counts are at least as accurate as the best methods of visual counting. The various technical difficulties encountered are discussed.

  14. Micropipette as Coulter counter for submicron particles (United States)

    Rudzevich, Yauheni; Ordonez, Tony; Evans, Grant; Chow, Lee


    Coulter counter based on micropipette has been around for several decades. Typical commercial Coulter counter has a pore size of 20 μ m, and is designed to detect micron-size blood cells. In recent years, there are a lot of interests in using nanometer pore size Coulter counter to detect single molecule and to sequence DNA. Here we describe a simple nanoparticle counter based on pulled micropipettes with a diameter of 50 -- 500 nm. Borosilicate micropipettes with an initial outer diameter of 1.00 mm and inner diameter of 0.5 mm are used. After pulling, the micropipettes are fire polished and ultrasound cleaned. Chlorinated Ag/AgCl electrodes and 0.1 M of KCl solution are used. The ionic currents are measured using an Axopatch 200B amplifier in the voltage-clamp mode. Several types and sizes of nanoparticles are measured, including plain silica and polystyrene nanospheres. The results will be discussed in terms of pH values of the solution and concentrations of the nanoparticles. Financial support from National Science Foundation (NSF-0901361) is acknowledged.

  15. Sensor and control for consistent seed drill coulter depth

    DEFF Research Database (Denmark)

    Kirkegaard Nielsen, Søren; Nørremark, Michael; Green, Ole


    The consistent depth placement of seeds is vital for achieving the optimum yield of agricultural crops. In state-of-the-art seeding machines, the depth of drill coulters will vary with changes in soil resistance. This paper presents the retrofitting of an angle sensor to the pivoting point...... by a sub-millimetre accurate positioning system (iGPS, Nikon Metrology NV, Belgium) mounted on the drill coulter. At a drill coulter depth of 55 mm and controlled by an ordinary fixed spring loaded down force only, the change in soil resistance decreased the mean depth by 23 mm. By dynamically controlling...

  16. Wallace H. Coulter: decades of invention and discovery. (United States)

    Robinson, J Paul


    Only a few inventors can be said to have made as great an impact on mankind as Wallace Coulter. His inquisitive mind and ability to see well beyond what existed served him well for 40 years of inventing. So many of the fundamental tools that exist today in the area of hematology were derived from or driven by Coulter's inventions that he could be called the most technological innovator in the field of modern hematology. In achieving these discoveries Wallace Coulter was clearly capable of visualizing future opportunities that few others recognized. His vision was combined with an uncanny ability to translate his ideas into products. He developed a large number of tools that shaped the fields of cytometry, image analysis, and industrial materials. His understanding of the future power of computation drove him to link these technologies in a unique way. In the end, Coulter shaped the technologies that ultimately drove hematology in a new direction, one that remains on a critical pathway linking technology innovation all the way to true translational impact. It was said of Henry Ford that "[h]e has no notion that wealth has made him great, and any one who is imprest merely by his wealth bores him. In his personal contacts he likes to dodge the subject. He would prefer to talk with a machinist about machinery, or with somebody who likes birds about birds. In these contacts, he asks no deference; and if he gets it, he suspects it is mere deference to wealth, and that ends his interest."(1) The same could be said of Wallace Coulter, who, like Ford, understood the concepts of mass production and customer service. Coulter had the ability to recognize the opportunity and fulfill the need for development of a blood-cell counter that could be placed in every pathology laboratory, and in so doing transformed a field from a qualitative to a quantitative environment. Every person who has ever entered a medical lab, hospital, or doctor's office has felt the impact of Coulter

  17. An extinction scale-expansion unit for the Beckman DK2 spectrophotometer (United States)

    Dixon, M.


    The paper describes a simple but accurate unit for the Beckman DK2 recording spectrophotometer, whereby any 0·1 section of the extinction (`absorbance') scale may be expanded tenfold, while preserving complete linearity in extinction. PMID:6048800

  18. Coulter dispersant as positive electrolyte additive for the vanadium redox flow battery

    International Nuclear Information System (INIS)

    Chang Fang; Hu Changwei; Liu Xiaojiang; Liu Lian; Zhang Jianwen


    Coulter dispersants were investigated as the additive into the positive electrolyte (more than 1.8 M vanadium ions) of vanadium redox flow battery (VRB). The electrolyte stability tests showed that, at 45, 50 and 60 °C, the addition of 0.050–0.10 w/w Coulter dispersant IIIA (mainly containing coconut oil amine adduct with 15 ethylene oxide groups) into the positive electrolyte of VRB could significantly delay the time of precipitate formation from 1.8–12.3 h to 30.3 h ∼ 19.3 days. Moreover, the trace amount of Coulter dispersant IIIA as the additive can enhance the electrolyte stability without changing the valence state of vanadium ions, reducing the reversibility of the redox reactions and incurring other side reactions at the electrode. Using the Coulter IIIA dispersant as the additive also improved the energy efficiency of the VRB. The UV–vis spectra confirmed that the trace amount of Coulter IIIA dispersant did not chemically react with V(V) to form new substances. The synergy of Coulombic repulsion and steric hindrance between the macromolecular cationic surfactant additive and the solution reduced the aggregation of vanadium ions into V 2 O 5 and increased the supersaturation of V 2 O 5 crystal in the solution.

  19. "Comments on Coulter and Smith": Relational Ontological Commitments in Narrative Research (United States)

    Clandinin, D. Jean; Murphy, M. Shaun


    In this comment article on Coulter and Smith (2009), the authors raise concerns that focusing exclusively on issues of representation may lead readers to misunderstandings about narrative research. The authors argue that narrative ways of thinking about the phenomena under study are interwoven with narrative research methodologies. Drawing on…

  20. Data transfer from Beckman LS 5800 liquid scintillation counters to IBM personal computers. (United States)

    Maan, A C


    This communication describes a short routine in BASICA for the IBM-PC, written to collect data from a Beckman liquid scintillation counter. In the form presented here the routine converts incoming bytes into separate lines and saves these lines in a file. There are many possible applications for further use of the data in these files. A few suggestions are given as to the format in which data can be stored and how to process these data automatically after all samples have been counted. The only hardware needed is an asynchronous communications adapter for the IBM-PC and an RS232 cable.

  1. Automation of a Beckman liquid scintillation counter for data capture and data-base management

    International Nuclear Information System (INIS)

    Neil, W.; Irwin, T.J.; Yang, J.J.


    A software package for the automation of a Beckman LS9000 liquid scintillation counter is presented. The package provides effective on-line data capture (with a Perkin Elmer 3230 32-bit minicomputer), data-base management, audit trail and archiving facilities. Key features of the package are rapid and flexible data entry, background subtraction, half-life correction, ability to queue several sample sets pending scintillation counting, and formatted report generation. A brief discussion is given on the development of customized data processing programs. (author)

  2. The Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin.

    LENUS (Irish Health Repository)

    Byrne, Brendan


    Commercially available prolactin immunoassays detect macroprolactin to variable degrees. Best practice requires laboratories to assess the cross-reactivity of their prolactin assay with macroprolactin, and where appropriate, introduce a screen for the presence of macroprolactin. Our policy has been to reanalyse hyperprolactinaemic samples following polyethylene glycol (PEG) precipitation and to report the resultant value as the monomeric prolactin content of the sample. The goal of this study was to determine the need to continue PEG precipitation when prolactin measurements with the Wallac AutoDELFIA were replaced by the Beckman DxI 800.

  3. Disturbance of Ultisol soil based on interactions between furrow openers and coulters for the no-tillage system

    Energy Technology Data Exchange (ETDEWEB)

    Francetto, T.R.; Alonço, A. dos S.; Brandelero, C.; Machado, O.D. da C.; Veit, A.A.; Carpes, D.P.


    The present study evaluated the effect of different associations between coulters and fertilizer furrow openers on soil disturbance, furrow depth and width, according to forward speed. The study was conducted on a farm in Santa Maria (Brazil/RS), in soil classified as sandy loam Ultisol. The experiment consisted of 24 combinations of treatments with three replications in a 2×3×4 factorial experiment. The combinations were formed by the interaction of the factors including: two types of furrow openers (hoe and double-disc), three types of coulters (no-coulter, smooth and offset fluted) and four levels of forward speed (1.11, 1.67, 2.22 and 2.78 m/s). Soil elevation and soil disturbance area profiles were obtained with the use of a micro profilometer, and disturbance values were calculated with the aid of computer software program Auto Cad. The disturbance area was not affected by speed; it was greater when using the hoe opener, and in association with the offset fluted coulter. Speed was inversely proportional to the depth of the furrows made by the hoe opener. Furthermore, the hoe caused the greatest furrow width (0.26 m) in comparison with the double-disc (0.24 m). The use of different coulters associated with furrow openers increased this variable (0.23 m for the no-coulter condition, 0.25 m with smooth and 0.26 m with offset fluted). The use of coulters combined with furrow openers reduces soil swelling, in approximately 8% for the smooth and 20% for the offset fluted. (Author)

  4. CLSI-based transference of the CALIPER database of pediatric reference intervals from Abbott to Beckman, Ortho, Roche and Siemens Clinical Chemistry Assays: direct validation using reference samples from the CALIPER cohort. (United States)

    Estey, Mathew P; Cohen, Ashley H; Colantonio, David A; Chan, Man Khun; Marvasti, Tina Binesh; Randell, Edward; Delvin, Edgard; Cousineau, Jocelyne; Grey, Vijaylaxmi; Greenway, Donald; Meng, Qing H; Jung, Benjamin; Bhuiyan, Jalaluddin; Seccombe, David; Adeli, Khosrow


    The CALIPER program recently established a comprehensive database of age- and sex-stratified pediatric reference intervals for 40 biochemical markers. However, this database was only directly applicable for Abbott ARCHITECT assays. We therefore sought to expand the scope of this database to biochemical assays from other major manufacturers, allowing for a much wider application of the CALIPER database. Based on CLSI C28-A3 and EP9-A2 guidelines, CALIPER reference intervals were transferred (using specific statistical criteria) to assays performed on four other commonly used clinical chemistry platforms including Beckman Coulter DxC800, Ortho Vitros 5600, Roche Cobas 6000, and Siemens Vista 1500. The resulting reference intervals were subjected to a thorough validation using 100 reference specimens (healthy community children and adolescents) from the CALIPER bio-bank, and all testing centers participated in an external quality assessment (EQA) evaluation. In general, the transferred pediatric reference intervals were similar to those established in our previous study. However, assay-specific differences in reference limits were observed for many analytes, and in some instances were considerable. The results of the EQA evaluation generally mimicked the similarities and differences in reference limits among the five manufacturers' assays. In addition, the majority of transferred reference intervals were validated through the analysis of CALIPER reference samples. This study greatly extends the utility of the CALIPER reference interval database which is now directly applicable for assays performed on five major analytical platforms in clinical use, and should permit the worldwide application of CALIPER pediatric reference intervals. Copyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  5. 93 Years of stand density and land-use legacy research at the Coulter Ranch Study Site (United States)

    Andrew J. Sanchez Meador; Margaret M. Moore


    In 1913, the Fort Valley Experimental Forest initiated an unprecedented case-study experiment to determine the effects of harvesting methods on tree regeneration and growth on a ponderosa pine-Gambel oak forest at Coulter Ranch in northern Arizona. The harvesting methods examined were seed-tree, group selection, and light selection. In addition, the effects of...

  6. 93 years of stand density and land-use legacy research at the Coulter Ranch Study Site (P-53) (United States)

    Andrew J. Sanchez Meador; Margaret M. Moore


    In 1913, the Fort Valley Experimental Forest initiated an unprecedented case-study experiment to determine the effects of harvesting methods on tree regeneration and growth on a ponderosa pine-Gambel oak forest at Coulter Ranch in northern Arizona. The harvesting methods examined were seed-tree, group selection, and light selection. In addition, the effects of...

  7. Observation and analysis of the Coulter effect through carbon nanotube and graphene nanopores. (United States)

    Agrawal, Kumar Varoon; Drahushuk, Lee W; Strano, Michael S


    Carbon nanotubes (CNTs) and graphene are the rolled and flat analogues of graphitic carbon, respectively, with hexagonal crystalline lattices, and show exceptional molecular transport properties. The empirical study of a single isolated nanopore requires, as evidence, the observation of stochastic, telegraphic noise from a blocking molecule commensurate in size with the pore. This standard is used ubiquitously in patch clamp studies of single, isolated biological ion channels and a wide range of inorganic, synthetic nanopores. In this work, we show that observation and study of stochastic fluctuations for carbon nanopores, both CNTs and graphene-based, enable precision characterization of pore properties that is otherwise unattainable. In the case of voltage clamp measurements of long (0.5-1 mm) CNTs between 0.9 and 2.2 nm in diameter, Coulter blocking of cationic species reveals the complex structuring of the fluid phase for confined water in this diameter range. In the case of graphene, we have pioneered the study and the analysis of stochastic fluctuations in gas transport from a pressurized, graphene-covered micro-well compartment that reveal switching between different values of the membrane permeance attributed to chemical rearrangements of individual graphene pores. This analysis remains the only way to study such single isolated graphene nanopores under these realistic transport conditions of pore rearrangements, in keeping with the thesis of this work. In summary, observation and analysis of Coulter blocking or stochastic fluctuations of permeating flux is an invaluable tool to understand graphene and graphitic nanopores including CNTs. © 2015 The Author(s).

  8. Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry

    Directory of Open Access Journals (Sweden)

    Jennifer Pasquier


    Full Text Available The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp. The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading, we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.

  9. Effects of particle's off-axis position, shape, orientation and entry position on resistance changes of micro Coulter counting devices

    International Nuclear Information System (INIS)

    Qin, Zhenpeng; Zhe, Jiang; Wang, Guo-Xiang


    With the recent advance in micro/nano-fabrication technology, micro Coulter counters have been widely used in detecting and characterizing micro- and nanoscale objects. In this paper, the electrical resistance change during translocation of a non-conducting particle through a channel is studied numerically. The numerical results are validated by proven analytical results available in the literature. The effects of particle's off-axis position, shape and orientation, and entry position are studied for particles with a large dynamic range. From the numerical results, a new fitted correlation is proposed that can accurately predict the resistance change caused by off-axis spherical particles regardless of their size. The shape and orientation effects of the electrical resistance change are studied by changing the axis ratio of spheroid particles and their orientation angles. Results show that a particle's shape and orientation have a significant influence on the resistance change. Simulation of an entry effect indicates that a particle starts to induce a resistance change before it enters the channel and still causes a resistance change even after the particle exits the channel completely. This study will offer some guidelines in designing and implementing Coulter counting devices and experiments, and provide insights into explaining experimental results

  10. Measurement of the volume growth rate of single budding yeast with the MOSFET-based microfluidic Coulter counter. (United States)

    Sun, Jiashu; Stowers, Chris C; Boczko, Erik M; Li, Deyu


    We report on measurements of the volume growth rate of ten individual budding yeast cells using a recently developed MOSFET-based microfluidic Coulter counter. The MOSFET-based microfluidic Coulter counter is very sensitive, provides signals that are immune from the baseline drift, and can work with cell culture media of complex composition. These desirable features allow us to directly measure the volume growth rate of single cells of Saccharomyces cerevisiae LYH3865 strain budding yeast in YNB culture media over a whole cell cycle. Results indicate that all budding yeast follow a sigmoid volume growth profile with reduced growth rates at the initial stage before the bud emerges and the final stage after the daughter gets mature. Analysis of the data indicates that even though all piecewise linear, Gomperitz, and Hill's function models can fit the global growth profile equally well, the data strongly support local exponential growth phenomenon. Accurate volume growth measurements are important for applications in systems biology where quantitative parameters are required for modeling and simulation.

  11. Field performance of a pneumatic row crop planter equipped with active toothed coulter for direct planting of corn in wheat residue

    Directory of Open Access Journals (Sweden)

    J. Nejadi


    Full Text Available In this research effects of active toothed coulter on planter penetration in semi-dried soils covered with previous wheat (triticum aestivum residue was studied. Therefore, effects of three levels of speed ratio (coulter peripheral speed/tractor forward speed; 1.22, 2, 3 at two previous residue levels (baled and non-baled and two modes of planter attachment (with and without row cleaner on the corn (zea mays planting depth and its uniformity, amount of surface residue, emergence rate index and seed spacings indices were investigated. Results showed that planting depth and its uniformity increased significantly with increasing speed ratio, so that desired planting depth was obtained at speed ratio of 2. The emergence rate index decreased with increase in speed ratio mainly due to increase in seeding depth at higher speed ratios. Miss and precision indices decreased in a significant manner with increase in the speed ratio. Other results showed that planting with row cleaner attachment into baled-out residue plots at speed ratio of 2 resulted in 31% decrease in miss and 30% decrease in precision indices as compared to control (inactive coulter. Quality of feed index at same conditions was 72%, the increase was up to 11% as compared to control. No significant difference was found between speed ratios of 2 and 3 for all the parameters studied. The results suggests that the row crop planter equipped with active toothed coulter and row cleaner attachment can be satisfactorily used for direct corn planting in hard soils covered with previous crop residues.

  12. Establishment of reference intervals for serum thyroid-stimulating hormone, free and total thyroxine, and free and total triiodothyronine for the Beckman Coulter DxI-800 analyzers by indirect method using data obtained from Chinese population in Zhejiang Province, China. (United States)

    Wang, Yan; Zhang, Yu-Xia; Zhou, Yong-Lie; Xia, Jun


    In order to establish suitable reference intervals of thyroid-stimulating hormone (TSH), free (unbound) T4 (FT4), free triiodothyronine (FT3), total thyroxine (T4), and total triiodothyronine (T3) for the patients collected in Zhejiang, China, an indirect method was developed using the data from the people presented for routine health check-up. Fifteen thousand nine hundred and fifty-six person's results were reviewed. Box-Cox or Case Rank was used to transform the data to normal distribution. Tukey and Box-Plot methods were used to exclude the outliers. Nonparametric method was used to establish the reference intervals following the EP28-A3c guideline. Pearson correlation was used to evaluate the correlation between hormone levels and age, while Mann-Whitney U test was employed for quantification of concentration differences on the people who are younger and older than 50 years old. Reference intervals were 0.66-4.95 mIU/L (TSH), 8.97-14.71 pmol/L (FT4), 3.75-5.81 pmol/L (FT3), 73.45-138.93 nmol/L (total T4), and 1.24-2.18 nmol/L (total T3) in male; conversely, reference intervals for female were 0.72-5.84 mIU/L (TSH), 8.62-14.35 pmol/L (FT4), 3.59-5.56 pmol/L (FT3), 73.45-138.93 nmol/L (total T4), and 1.20-2.10 nmol/L (total T3). FT4, FT3, and total T3 levels in male and FT4 level in female had an inverse correlation with age. Total T4 and TSH levels in female were directly correlated. Significant differences in these hormones were also found between younger and older than 50 years old except FT3 in female. Indirect method can be applied for establishment of reference intervals for TSH, FT4, FT3, total T4, and total T3. The reference intervals are narrower than those previously established. Age factor should also be considered. © 2016 Wiley Periodicals, Inc.

  13. Gastroprotective effect of diligustilide isolated from roots of Ligusticum porteri coulter & rose (Apiaceae) on ethanol-induced lesions in rats. (United States)

    Velázquez-Moyado, Josué A; Martínez-González, Alejandro; Linares, Edelmira; Bye, Robert; Mata, Rachel; Navarrete, Andrés


    The rhizome of Ligusticum porteri Coulter& Rose (LP) has been traditionally used by the ethnic group Raramuri in the North of México for treatment of diabetes, tuberculosis, stomachaches, diarrhea and ritual healing ceremonies. It is use as antiulcer remedy has been extended to all Mexico. To evaluate the gastroprotective activity of LP organic extracts and the major natural product diligustilide (DLG),using as experimental model the inhibition of the ethanol-induced lesions in rats. Gastric ulcers were induced by intragastric instillation of absolute ethanol (1 mL). We tested the gastroprotective activity of the organic extracts of LP and the pure compound DLG. The ulcer index (UI) was determined to measure the activity. In order to elucidate the action mechanism of DLG the animals were treated with L-NAME, N-ethylmalemide, Forskolin, 2',5'-dideoxyadenosine, Indomethacin, Glibenclameide, Diazoxide, NaHS and DL-Propargylglycine. The pylorus-ligated rat model was used to measure gastric secretion. The oral administration of organic extracts of Ligusticum porteri showed gastroprotective effect at 30 mg/Kg on ethanol induced gastric lesions; hexane and dichloromethane extracts were the most active. DLG was the major compound in the hexane extract. This compound at 10 mg/kg prevented significantly the gastric injuries induced by ethanol. The alkylation of endogenous non-protein-SH groups with N-ethylmaleimide abolished the gastroprotective effect of DLG and blocking the formation of endogenous prostaglandins by the pretreatment with indomethacin attenuated the gastroprotective effect of DLG. The gastroprotective activity demonstrated in this study tends to support the ethnomedical use of Ligusticum porteri roots. DLG, isolated as major compound of this medicinal plant has a clear gastroprotective effect on the ethanol-induced gastric lesions. The results suggest that the antiulcer activity of DLG depends on the participation of the endogenous non-protein -SH groups

  14. Creating libraries for commercial yeast strains through miniaturization of cloning and transformations using the BioRAPTR FRD Microfluidic workstation (United States)

    The ability to miniaturize molecular reactions can lead to significant cost savings when creating libraries of thousands of clones. For this application Beckman Coulter partnered with the USDA to provide a low-volume automated solution for library cloning for use in the development of yeast strains...

  15. Enough room for Williams and IMF? / Paul Beckman

    Index Scriptorium Estoniae

    Beckman, Paul


    Lõppesid Leedu ja USA energeetikakompanii Williams International läbirääkimised Leedu naftakompleksis osaluse omandamise asjus. IMF uurib Leedu majanduslikku arengut, mida tehing Williamsiga komplitseerib


    Directory of Open Access Journals (Sweden)

    Rogério de Araújo Almeida


    the State of Goiás, Brazil. Two levels of mulching, three soil opener systems, and two coulter settings were compared. None of the treatments met the agronomic requirements for the black bean direct drilling. Lower mass and density of cover crop residues resulted in better seed distribution and higher plant population. The system with coulter fitted in the depth limiting wheel favored deeper fertilizer and resulted in lower uncovered seed percentage. Double disc opener presented shallower depth of fertilizer and more uncovered seeds, carrying out a lower plant population.

    KEY-WORDS: Zero tillage; planter machine; animal traction.

  17. Delayed Treatment of Ebola Virus Infection with Plant-Derived Monoclonal Antibodies Provides Protection in Rhesus Macaques (United States)


    filtration (Mustang Q membrane ; Pall). The final polishing column for c13C6 and c6D8 was a CHT, Type I 40-μm (Bio-Rad) column. The columnwas...monkey chow, primate treats, fruits, and vegetables throughout the course of the study. Animals were observed at least once daily to monitor overall...Coulter Act 10 (Beckman Coulter) on samples collected in EDTA plasma vacuette tubes (Greiner Bio-One). Blood was collected in 2-mL serum vacuette tubes

  18. In vivo hepatic differentiation potential of human umbilical cord-derived mesenchymal stem cells: Therapeutic effect on liver fibrosis/cirrhosis


    Zhang, Guo-Zun; Sun, Hui-Cong; Zheng, Li-Bo; Guo, Jin-Bo; Zhang, Xiao-Lan


    AIM To investigate the hepatic differentiation potential of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) and to evaluate their therapeutic effect on liver fibrosis/cirrhosis. METHODS A CCl4-induced liver fibrotic/cirrhotic rat model was used to assess the effect of hUC-MSCs. Histopathology was assessed by hematoxylin and eosin (H&E), Masson trichrome and Sirius red staining. The liver biochemical profile was measured using a Beckman Coulter analyzer. Expression analysis was ...

  19. The Utilization of Triton X-100 for Enhanced Two-Dimensional Liquid-Phase Proteomics


    Kim, Mina; Lee, Sang-Hee; Min, Jiho; Kobayashi, Fumihisa; Um, Hyun-Ju; Kim, Yang-Hoon


    One of the main challenges in proteomics lies in obtaining a high level of reproducible fractionation of the protein samples. Automated two-dimensional liquid phase fractionation (PF2D) system manufactured by Beckman Coulter provides a process well suited for proteome studies. However, the protein recovery efficiency of such system is low when a protocol recommended by the manufacturer is used for metaproteome profiling of environmental sample. In search of an alternative method that can over...

  20. The NUP98 Gene as a Potential Modifier of NF2 Associated Tumors (United States)


    hemopoietic stem cells. J Immunol 174:6477–6489 32. Evans MS, Chaurette JP, Adams ST Jr, Reddy GR, Paley MA, Aronin N, Prescher JA, Miller SC (2014) A...vascular endothelial growth factor receptor 1-3), Ret (rearranged during transfection) and KIT (mast/ stem cell growth factor receptor) [19]. Ponatinib... strands using the ABI Prism BigDye Terminator reaction kit and ABI 3100 DNA sequencer or with Beckman Coulter 2000 XL instrument and appropriate kits

  1. Conversion of Beckman DK-2A spectrophotometer into an automatic single-photon counting fluorescence spectrophotometer

    International Nuclear Information System (INIS)

    Chikkur, G.C.; Lagare, M.T.; Umakantha, N.


    Details of how a DK-2A spectrophotometer can be modified into an automatic single-photon counting fluorescence spectrophotometer for recording a low intensity spectrum, are reported. The single-photon count-rate converted into a DC voltage is applied at the appropriate stage in the sample channel amplifier circuit of a DK-2A to get the pen deflection proportional to the count-rate. A high intensity spectrum may be recorded in the usual way by merely turning the shaft of the mirror motor by 180 degrees. (author)

  2. The Beckman-Quarles theorem for continuous mappings from R^n to C^n


    Tyszka, Apoloniusz


    Let \\phi((x_1,...,x_n),(y_1,...,y_n))=(x_1-y_1)^2+...+(x_n-y_n)^2. We say that f:R^n -> C^n preserves distance d>=0 if for each x,y \\in R^n \\phi(x,y)=d^2 implies \\phi(f(x),f(y))=d^2. We prove that if x,y \\in R^n (n>=3) and |x-y|=(\\sqrt{2+2/n})^k \\cdot (2/n)^l (k,l are non-negative integers) then there exists a finite set {x,y} \\subseteq S(x,y) \\subseteq R^n such that each unit-distance preserving mapping from S(x,y) to C^n preserves the distance between x and y. It implies that each continuou...

  3. Comparación del contaje plaquetario empleando diferentes metodologías, en pacientes con púrpura trombocitopénica y síndromes mielodisplásicos


    Zabala, Norielis; Hann aoui, Erika


    Se valoró el contaje plaquetario de tres grupos de pacientes, de acuerdo al método manual de Brecher y Cronkite y al método automatizado, usando analizadores hematológicos con diferentes principios: Swelab ALFA (impedancia), Cell-dyn 3200 (citometría de flujo) y Beckman Coulter LH 500 (impedancia volumétrica, radiofrecuencia y dispersión de la luz láser). Los pacientes con púrpura trombocitopénica (PT) y síndromes mielodisplásicos (SMD), provionieron de la consulta de hematología del Hospital...

  4. Typing of 48 autosomal SNPs and amelogenin with GenPlex SNP genotyping system in forensic genetics

    DEFF Research Database (Denmark)

    Tomas Mas, Carmen; Stangegaard, Michael; Børsting, Claus


    , Somalia and Greenland were investigated with GenPlex using a Biomek 3000 (Beckman Coulter) robot. The results were compared to results obtained with an ISO 17025 accredited SNP typing assay based on single base extension (SBE). With the GenPlex SNP genotyping system, full SNP profiles were obtained in 97.......6% of the investigations. Perfect concordance was obtained in duplicate investigations and the SNP genotypes obtained with the GenPlex system were concordant with those of the accredited SBE based SNP typing system except for one result in rs901398 in one of 286 individuals most likely due to a mutation 6 bp downstream...

  5. A European multicenter study on the analytical performance of the VERIS HBV assay. (United States)

    Braun, Patrick; Delgado, Rafael; Drago, Monica; Fanti, Diana; Fleury, Hervé; Izopet, Jacques; Lombardi, Alessandra; Mancon, Alessandro; Marcos, Maria Angeles; Sauné, Karine; O Shea, Siobhan; Pérez-Rivilla, Alfredo; Ramble, John; Trimoulet, Pascale; Vila, Jordi; Whittaker, Duncan; Artus, Alain; Rhodes, Daniel

    Hepatitis B viral load monitoring is an essential part of managing patients with chronic Hepatits B infection. Beckman Coulter has developed the VERIS HBV Assay for use on the fully automated Beckman Coulter DxN VERIS Molecular Diagnostics System. 1 OBJECTIVES: To evaluate the analytical performance of the VERIS HBV Assay at multiple European virology laboratories. Precision, analytical sensitivity, negative sample performance, linearity and performance with major HBV genotypes/subtypes for the VERIS HBV Assay was evaluated. Precision showed an SD of 0.15 log 10 IU/mL or less for each level tested. Analytical sensitivity determined by probit analysis was between 6.8-8.0 IU/mL. Clinical specificity on 90 unique patient samples was 100.0%. Performance with 754 negative samples demonstrated 100.0% not detected results, and a carryover study showed no cross contamination. Linearity using clinical samples was shown from 1.23-8.23 log 10 IU/mL and the assay detected and showed linearity with major HBV genotypes/subtypes. The VERIS HBV Assay demonstrated comparable analytical performance to other currently marketed assays for HBV DNA monitoring. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Prostate health index (phi) and prostate cancer antigen 3 (PCA3) significantly improve diagnostic accuracy in patients undergoing prostate biopsy. (United States)

    Perdonà, Sisto; Bruzzese, Dario; Ferro, Matteo; Autorino, Riccardo; Marino, Ada; Mazzarella, Claudia; Perruolo, Giuseppe; Longo, Michele; Spinelli, Rosa; Di Lorenzo, Giuseppe; Oliva, Andrea; De Sio, Marco; Damiano, Rocco; Altieri, Vincenzo; Terracciano, Daniela


    Prostate health index (phi) and prostate cancer antigen 3 (PCA3) have been recently proposed as novel biomarkers for prostate cancer (PCa). We assessed the diagnostic performance of these biomarkers, alone or in combination, in men undergoing first prostate biopsy for suspicion of PCa. One hundred sixty male subjects were enrolled in this prospective observational study. PSA molecular forms, phi index (Beckman coulter immunoassay), PCA3 score (Progensa PCA3 assay), and other established biomarkers (tPSA, fPSA, and %fPSA) were assessed before patients underwent a 18-core first prostate biopsy. The discriminating ability between PCa-negative and PCa-positive biopsies of Beckman coulter phi and PCA3 score and other used biomarkers were determined. One hundred sixty patients met inclusion criteria. %p2PSA (p2PSA/fPSA × 100), phi and PCA3 were significantly higher in patients with PCa compared to PCa-negative group (median values: 1.92 vs. 1.55, 49.97 vs. 36.84, and 50 vs. 32, respectively, P ≤ 0.001). ROC curve analysis showed that %p2PSA, phi, and PCA3 are good indicator of malignancy (AUCs = 0.68, 0.71, and 0.66, respectively). A multivariable logistic regression model consisting of both the phi index and PCA3 score allowed to reach an overall diagnostic accuracy of 0.77. Decision curve analysis revealed that this "combined" marker achieved the highest net benefit over the examined range of the threshold probability. phi and PCA3 showed no significant difference in the ability to predict PCa diagnosis in men undergoing first prostate biopsy. However, diagnostic performance is significantly improved by combining phi and PCA3. Copyright © 2012 Wiley Periodicals, Inc.

  7. Two new automated, compared with two enzyme-linked immunosorbent, antimüllerian hormone assays. (United States)

    Nelson, Scott M; Pastuszek, Ewa; Kloss, Grzegorz; Malinowska, Iwona; Liss, Joanna; Lukaszuk, Aron; Plociennik, Lukasz; Lukaszuk, Krzysztof


    To compare new automated antimüllerian hormone (AMH) assay performance characteristics from the new automated Elecsys AMH (Roche; Elecsys) and Access AMH (Beckman Coulter; Access) assays with the existing AMH Gen II ELISA (enzyme-linked immunosorbent assay; Gen II; Beckman Coulter) and AMH ELISA (Ansh Labs) assays. Prospective assay evaluation. University-affiliated clinical chemistry laboratory. Patients referred for serum AMH measurement (n = 83) before start of in vitro fertilization cycle between September 2014 and October 2014. None. Serum AMH concentration. Intra-assay coefficients of variation were low; Ansh ≤ 9.0%; Gen II ≤ 5.8%; Access ≤ 10.7%; and Elecsys ≤ 2.8%. The Passing-Bablok regression equations (pmol/L) were y (Access) = 0.128 + (0.781 × Gen II); and y (Access) = 0.302 + (0.742 x Ansh). For y (Elecys) = 0.087 + (0.729 x Gen II) and y (Elecys) = 0.253 + (0.688 x Ansh Labs). For y (Elecys) = 0.943 - (0.037 × Access). For all the assays, AMH exhibited a moderate positive correlation with AFC (r = 0.62-0.64); number of cumulus oocyte complexes (r = 0.60-0.64); and metaphase II oocytes (r = 0.48-0.50). Accuracy of pregnancy prediction, as determined by area under the receiver operating characteristic curve, was uniformly low for all assays (0.62-0.63). The novel automated assays exhibit strong concordance in calibration, but derived values are substantially lower than those obtained from pre-existing assays, with assay-specific interpretation required for routine clinical use. These results highlight the need for an international standard of measurement of AMH. Copyright © 2015 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  8. [Hematologic indices in different age wistar rats, receiving a balanced semi-synthetic vivary diet]. (United States)

    Mustafina, O K; Trushina, É N; Shumakova, E A; Arianova, E A; Tyshko, N V; Pashorina, V A


    This paper presents the results of research of hematologic parameters of male Wistar rats 1, 2, 3, 4 and 6 months age, which received a balanced semisynthetic diet. Studies were carried out at the Hematology analyzer Coulter AC TTM 5 diff OV (Beckman Coulter, USA) with the program, specially developed for the study of rats' blood. According to the results of research, was found a statistically significant increased of the number of red blood cells; the concentration of hemoglobin and hematocrit in animals 2-6 months compared with rats, 1 month age. With age, there is a decrease of the mean corpuscular volume and the mean corpuscular hemoglobin. The number of white blood cells in rats of 2-4 months age are significantly higher than in rats of 1 and 6 months age. The number of neutrophils and eosinophils in rats of to the 2 month are of is lover than once in rats of 1 month age, and increases values in animals of 6 months age. The number of lymphocytes has the highest value in the rat of 2-3 months age and the minimum value is that in animals of 6 months age. With increasing of the age of the animals the reduction of contents of monocytes was noted. The content of platelets and the platelet crit in the blood of rats 6 months age is statistically greater than those in 1-month age animals. The average volume of platelet is the stable index, with age does not change.

  9. In Vitro toxicological effects of Fumonisin B1 and Beauvericin on bovine granulosa cells

    Directory of Open Access Journals (Sweden)

    Marco Albonico


    Full Text Available Fumonisin B1 (FB1 and beauvericin (BEA are fusariotoxins found to co-exist in food and feed commodities. The aim of this study is to evaluate the individual and combined effects of FB1 and BEA on bovine granulosa cell proliferation and steroid production. Granulosa cells (GC from small bovine follicles (1-5 mm were cultured for 48 hours in 10% fetal bovine serum followed by 48 hours in a serum-free medium containing 500 ng/ml of testosterone (as an estradiol precursor, 30 ng/ml of FSH and 30 ng/ml of IGF-I with and without FB1 (3 µM and BEA (3 µM. At the end of the experiment, the numbers of GC were determined using a Coulter counter (Beckman Coulter, USA and concentrations of progesterone and estradiol in the culture medium were determined by radioimmunoassay. FB1 and BEA, both individually and in combination, showed an inhibitory effect (P 0.05 on estradiol and progesterone production, whereas BEA (3 µM, both alone and in combination with FB1 (3 µM, was found to decrease (P < 0.001 the production of both steroids drastically. In conclusion, this in vitro study indicates that FB1 and BEA, both individually and in combination, may affect GC proliferation to different extents and shows the drastic inhibitory effects of BEA on steroid production.


    Directory of Open Access Journals (Sweden)

    T. F. Sokolova


    Full Text Available Today the questions about possibility of development disturbances in the immune system of the fetus and the newborn in chronic toxoplasmosis are poorly understood. Aim of research: to detect immunological disturbances in the offspring of rats which have been administered antigens T. gondii.Two series of experiments was performed. In these experiments white female Wistar rats in the III trimester of pregnancy have been administered corpuscular antigen T. gondii. The 60 days-old offspring of these rats have been included in study group of 137 animals. CD3+ cells count was performed in peripherical blood and standard suspension of splenocytesrats offspring. Peripherical blood cells count was performed in the blood of the rats offspring. In the second experiment rats offspring have been administered sheep erythrocytes in 5 days, before euthanasia. In spleen of this rats antigen-produced cells was counted.In control group was included 118 animals, which was born from white female Wistar rats have been administered 0,9% NaCl solution. CD3+ cells was detected in Cytomics FC500 flow cytometry analyzer (Beckman Coulter,USA by use rats origne-specifed monoclonal antibodies Anti-Rat CD3-FITC (Beckman Coulter,USA. Hematological parameters was assessed by use hematological analyzer Excell-22 (USA.We observed, that CD3+ lymphocytes and antigen-produced cells was decreased in test group (degress of decrease CD3+ cells was 17,2%; р = 0,003 in spleen vs. control group, degress of decrease antigen-produced cells was 27,3%; р = 0,03 vs. control group. Number of leukocytes was increased in in test group (34,5%; р = 0,009 vs. control group. Power and strength correlation pleiades between studied blood and spenal markers were higher in in test group vs. control group (∑Gi = 16; ∑Di = 4,38 vs. ∑Gi = 13; ∑Di = 2,28. This phenomenon is probably due to the development adaptive reactions disruption in the immune system and development

  11. Autovalidation and automation of the postanalytical phase of routine hematology and coagulation analyses in a university hospital laboratory. (United States)

    Mlinaric, Ana; Milos, Marija; Coen Herak, Désirée; Fucek, Mirjana; Rimac, Vladimira; Zadro, Renata; Rogic, Dunja


    The need to satisfy high-throughput demands for laboratory tests continues to be a challenge. Therefore, we aimed to automate postanalytical phase in hematology and coagulation laboratory by autovalidation of complete blood count (CBC) and routine coagulation test results (prothrombin time [PT], international normalized ratio [PT-INR], activated partial thromboplastin time [APTT], fibrinogen, antithrombin activity [AT] and thrombin time [TT]). Work efficacy and turnaround time (TAT) before and after implementation of automated solutions will be compared. Ordering panels tailored to specific patient populations were implemented. Rerun and reflex testing rules were set in the respective analyzers' software (Coulter DxH Connectivity 1601, Beckman Coulter, FL, USA; AutoAssistant, Siemens Healthcare Diagnostics, Germany), and sample status information was transferred into the laboratory information system. To evaluate if the automation improved TAT and efficacy, data from manually verified results in September and October of 2015 were compared with the corresponding period in 2016 when autovalidation was implemented. Autovalidation rates of 63% for CBC and 65% for routine coagulation test results were achieved. At the TAT of 120 min, the percentage of reported results increased substantially for all analyzed tests, being above 90% for CBC, PT, PT-INR and fibrinogen and 89% for APTT. This output was achieved with three laboratory technicians less compared with the period when the postanalytical phase was not automated. Automation allowed optimized laboratory workflow for specific patient populations, thereby ensuring standardized results reporting. Autovalidation of test results proved to be an efficient tool for improvement of laboratory work efficacy and TAT.

  12. An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems

    Directory of Open Access Journals (Sweden)

    Verschraegen Gerda


    Full Text Available Abstract Background Currently, most laboratories identify yeasts routinely on the basis of morphology and biochemical reactivity. This approach has quite often limited discriminatory power and may require long incubation periods. Due to the increase of fungal infections and due to specific antifungal resistence patterns for different species, accurate and rapid identification has become more important. Several molecular techniques have been described for fast and reliable identification of yeast isolates, but interlaboratory exchangeability of identification schemes of molecular techniques has hardly been studied. Here, we compared amplified ITS2 fragment length determination by an ABI Prism 310 (Applied Biosystems, Foster City, Ca. capillary electrophoresis system with that obtained by a CEQ8000 (Beckman Coulter, Fullerton, Ca. capillary electrophoresis system. Results Although ITS2 size estimations on both systems differed and separate libraries had to be constructed for each system, both approaches had the same discriminatory power with regard to the 44 reference strains, identical identifications were obtained for 39/ 40 clinical isolates in both laboratories and strains from 51 samples were correctly identified using CEQ8000, when compared to phenotypic identification. Conclusion Identification of yeasts with ITS2-PCR followed by fragment analysis can be carried out on different capillary electrophoresis systems with comparable discriminatory power.

  13. In vivo hepatic differentiation potential of human umbilical cord-derived mesenchymal stem cells: Therapeutic effect on liver fibrosis/cirrhosis. (United States)

    Zhang, Guo-Zun; Sun, Hui-Cong; Zheng, Li-Bo; Guo, Jin-Bo; Zhang, Xiao-Lan


    To investigate the hepatic differentiation potential of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) and to evaluate their therapeutic effect on liver fibrosis/cirrhosis. A CCl 4 -induced liver fibrotic/cirrhotic rat model was used to assess the effect of hUC-MSCs. Histopathology was assessed by hematoxylin and eosin (H&E), Masson trichrome and Sirius red staining. The liver biochemical profile was measured using a Beckman Coulter analyzer. Expression analysis was performed using immunofluorescent staining, immunohistochemistry, Western blot, and real-time PCR. We demonstrated that the infused hUC-MSCs could differentiate into hepatocytes in vivo . Functionally, the transplantation of hUC-MSCs to CCl 4 -treated rats improved liver transaminases and synthetic function, reduced liver histopathology and reversed hepatobiliary fibrosis. The reversal of hepatobiliary fibrosis was likely due to the reduced activation state of hepatic stellate cells, decreased collagen deposition, and enhanced extracellular matrix remodeling via the up-regulation of MMP-13 and down-regulation of TIMP-1. Transplanted hUC-MSCs could differentiate into functional hepatocytes that improved both the biochemical and histopathologic changes in a CCl 4 -induced rat liver fibrosis model. hUC-MSCs may offer therapeutic opportunities for treating hepatobiliary diseases, including cirrhosis.

  14. Predicting prostate biopsy outcome: prostate health index (phi) and prostate cancer antigen 3 (PCA3) are useful biomarkers. (United States)

    Ferro, Matteo; Bruzzese, Dario; Perdonà, Sisto; Mazzarella, Claudia; Marino, Ada; Sorrentino, Alessandra; Di Carlo, Angelina; Autorino, Riccardo; Di Lorenzo, Giuseppe; Buonerba, Carlo; Altieri, Vincenzo; Mariano, Angela; Macchia, Vincenzo; Terracciano, Daniela


    Indication for prostate biopsy is presently mainly based on prostate-specific antigen (PSA) serum levels and digital-rectal examination (DRE). In view of the unsatisfactory accuracy of these two diagnostic exams, research has focused on novel markers to improve pre-biopsy prostate cancer detection, such as phi and PCA3. The purpose of this prospective study was to assess the diagnostic accuracy of phi and PCA3 for prostate cancer using biopsy as gold standard. Phi index (Beckman coulter immunoassay), PCA3 score (Progensa PCA3 assay) and other established biomarkers (tPSA, fPSA and %fPSA) were assessed before a 18-core prostate biopsy in a group of 251 subjects at their first biopsy. Values of %p2PSA and phi were significantly higher in patients with PCa compared with PCa-negative group (pphi and PCA3 are predictive of malignancy. In conclusion, %p2PSA, phi and PCA3 may predict a diagnosis of PCa in men undergoing their first prostate biopsy. PCA3 score is more useful in discriminating between HGPIN and non-cancer. Copyright © 2012 Elsevier B.V. All rights reserved.

  15. The prostate health index PHI predicts oncological outcome and biochemical recurrence after radical prostatectomy - analysis in 437 patients. (United States)

    Maxeiner, Andreas; Kilic, Ergin; Matalon, Julia; Friedersdorff, Frank; Miller, Kurt; Jung, Klaus; Stephan, Carsten; Busch, Jonas


    The purpose of this study was to investigate the Prostate-Health-Index (PHI) for pathological outcome prediction following radical prostatectomy and also for biochemical recurrence prediction in comparison to established parameters such as Gleason-score, pathological tumor stage, resection status (R0/1) and prostate-specific antigen (PSA). Out of a cohort of 460 cases with preoperative PHI-measurements (World Health Organization calibration: Beckman Coulter Access-2-Immunoassay) between 2001 and 2014, 437 patients with complete follow up data were included. From these 437 patients, 87 (19.9%) developed a biochemical recurrence. Patient characteristics were compared by using chi-square test. Predictors were analyzed by multivariate adjusted logistic and Cox regression. The median follow up for a biochemical recurrence was 65 (range 3-161) months. PHI, PSA, [-2]proPSA, PHI- and PSA-density performed as significant variables (p PHI, PSA, %fPSA, [-2]proPSA, PHI- and PSA-density significantly discriminated between stages PHI. In biochemical recurrence prediction PHI, PSA, [-2]proPSA, PHI- and PSA-density were the strongest predictors. In conclusion, due to heterogeneity of time spans to biochemical recurrence, longer follow up periods are crucial. This study with a median follow up of more than 5 years, confirmed a clinical value for PHI as an independent biomarker essential for biochemical recurrence prediction.

  16. Performance evaluation of Samsung LABGEO(HC10) Hematology Analyzer. (United States)

    Park, Il Joong; Ahn, Sunhyun; Kim, Young In; Kang, Seon Joo; Cho, Sung Ran


    The Samsung LABGEO(HC10) Hematology Analyzer (LABGEO(HC10)) is a recently developed automated hematology analyzer that uses impedance technologies. The analyzer provides 18 parameters including 3-part differential at a maximum rate of 80 samples per hour. To evaluate the performance of the LABGEO(HC10). We evaluated precision, linearity, carryover, and relationship for complete blood cell count parameters between the LABGEO(HC10) and the LH780 (Beckman Coulter Inc) in a university hospital in Korea according to the Clinical and Laboratory Standards Institute guidelines. Sample stability and differences due to the anticoagulant used (K₂EDTA versus K₃EDTA) were also evaluated. The LABGEO(HC10) showed linearity over a wide range and minimal carryover ( 0.92) except for mean corpuscular hemoglobin concentration. The bias estimated was acceptable for all parameters investigated except for monocyte count. Most parameters were stable until 24 hours both at room temperature and at 4°C. The difference by anticoagulant type was statistically insignificant for all parameters except for a few red cell parameters. The accurate results achievable and simplicity of operation make the unit recommendable for small to medium-sized laboratories.

  17. Ultrasonic sonoelastography of scrotum in the diagnosis of male fertility

    Directory of Open Access Journals (Sweden)

    O. B. Zhukov


    Full Text Available Objective: to determine the possibility of using detect the scrotum in assessing reproductive function of men.Materials and methods. The study was conducted in men aged 20–44 years. The first group of men was 25 patients with secretory infertility. The second group included 25 male infertility varicocele. The control group presents 12 men in the age range 22–31 year with the implementation of the reproductive function of 1 or more children. The study was performed by ultrasonic scanner expert class Acuson S2000 manufacture of the company Siemens, was used microwave sensor 18L6 HD. Sex hormones were studied on immunochemiluminescence analyzer Access 2 production Beckman Coulter (USA. Obtaining and study of ejaculate was carried out according to Recommendations of the WHO (5th edition.Results. The application of research shear transverse wave Vs men with secretory infertility and varicocele is accompanied by the increase of its value to 1.5–1.6 m/s and is characterized by 35–45 % from similar parameters of reproductive healthy men. In half of the cases studies such an increase in performance and decrease in the area of mapping the so-called “soft” areas of the epididymis is accompanied by a decrease in the concentration and actively motile sperm. The use of such method of instrumental diagnostics will allow at the stage of preliminary ultrasound screening to suspect a violation of the reproductive capacity of men.

  18. Biological Reference Interval for Hematological Profile of Umbilical Cord Blood: A Study Conducted at A Tertiary Care Centre in South India (United States)

    Raj, Reddy S. Sudheer; Priyathersini, N.; Rajendran, Rithika; Rajendran, Rashmika; Ramadoss, Umalakshmi


    Introduction Umbilical cord blood (UCB) a source of hematopoietic stem cells, is also an acceptable sample to assess neonatal sepsis. Though reports are available for stem cell counts very minimal literature is available regarding hematologic parameters, which may vary on ethnicity. Aim To establish biological reference interval for hematological parameters of umbilical cord blood to guide neonatologists, hematopoietic stem cell transplant specialists and future analysis. Materials and Methods Prospective longitudinal study was done from January 2014 to April 2014 after ethics committee approval. UCB from 120 full term new borns of normal birth weight born out of uneventful pregnancy to mothers aged between 21 to 45 years with hemoglobin above 10g/dL were processed in Beckman Coulter LH780 analyzer for complete blood count and counter checked by peripheral smear. Results tabulated in Microsoft excel are analyzed using IBM SPSS statistics 16 software. Results Male to female ratio is 1:1.05. There is no difference in the values between males and females. When compared with few studies available, though many values are comparable a few values are not comparable. Conclusion This study can be a useful guide to neonatologists, hematopoietic stem cells transplant hematologists and future analysis. PMID:26557584

  19. Anti-Müllerian hormone (AMH) levels in premenopausal breast cancer patients treated with taxane-based adjuvant chemotherapy - A translational research project of the SUCCESS A study. (United States)

    Trapp, Elisabeth; Steidl, J; Rack, B; Kupka, M S; Andergassen, U; Jückstock, J; Kurt, A; Vilsmaier, T; de Gregorio, A; de Gregorio, N; Tzschaschel, M; Lato, C; Polasik, A; Tesch, H; Schneeweiss, A; Beckmann, M W; Fasching, P A; Janni, W; Müller, V


    Premenopausal women undergoing chemotherapy are at high risk for premature ovarian failure and its long-term consequences. Data on potential markers to evaluate ovarian reserve pre- and posttreatment are limited. Anti-Müllerian hormone (AMH) known for ovarian reserve in reproductive medicine could be a surrogate marker and was assessed in premenopausal breast cancer patients of the SUCCESS A study (EUDRA-CT no. 2005-000490-21). We identified 170 premenopausal patients, age ≤ 40 years at trial entry, who received FEC-Doc as taxane-anthracylince based chemotherapy. Blood samples were taken at three time points: Before, four weeks after and two years after adjuvant chemotherapy. Serum AMH-levels were evaluated in a central laboratory by a quantitative immunoassay AMH Gen II ELISA (Beckman Coulter, Brea, USA). Median age was 36 years (21-40 years). Median serum AMH-level before chemotherapy was 1.37 ng/ml (range chemotherapy AMH-levels dropped in 98.6% of the patients to chemotherapy induced amenorrhea occurred only in 50.6% of the patients. In this analysis, premenopausal patients showed a high rate of ovarian impairment reflected by low AMH-levels after chemotherapy. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Metabolic changes during development of Walker-256 carcinosarcoma resistance to doxorubicin. (United States)

    Todor, I N; Lukyanova, N Yu; Shvets, Yu V; Lozovska, Yu V; Chekhun, V F


    To study indices of energy metabolism, content of K(+) and Mg(++) both in peripheral blood and in Walker-256 carcinosarcoma during development of resistance to doxorubicin. Resistance of Walker-256 carcinosarcoma to doxorubicin has been developed through 12 subsequent transplantations of tumor after the chemotherapy. Parental strain was inhibited by drug by 65%, while transitional resistant substrains - by 30% and 2%, respectively. Determination of biochemical indices in blood serum and homogenates of tumor tissue, level of potassium, magnesium, lactate, glucose, activities of lactate dehydrogenase and glucose-6-phosphate dehydrogenase was performed with the help of biochemical and immune-enzyme analyzer GBG ChemWell 2990 (USA) using standard kits. Polarography was used to determine indices of mitochondrial oxidative phosphorylation. Study of mitochondrial membrane potential was carried out on flow cytometer Beckman Coulter Epics XL using dye JC-1. It has been determined that development of drug resistance causes the decrease of K(+), Mg(++), glucose content in blood serum and increase of these indices in tumor tissue. At the same time, gradual tumor's loss of sensitivity is characterized by decrease of glycolysis activity in it and activation of mitochondrial oxidative phosphorylation and pentose phosphate pathway of glucose degradation, which causes more intensive formation of NADPH. Development of drug resistance of tumor causes certain metabolic changes in organism and tumor. Further study of such changes will make possible to determine tumor and extratumor markers of resistance.

  1. Multicenter evaluation of an enzymatic method for glycated albumin. (United States)

    Paleari, Renata; Bonetti, Graziella; Callà, Cinzia; Carta, Mariarosa; Ceriotti, Ferruccio; Di Gaetano, Nicola; Ferri, Marilisa; Guerra, Elena; Lavalle, Gabriella; Cascio, Claudia Lo; Martino, Francesca Gabriela; Montagnana, Martina; Moretti, Marco; Santini, Gabriele; Scribano, Donata; Testa, Roberto; Vero, Anna; Mosca, Andrea


    The use of glycated albumin (GA) has been proposed as an additional glycemic control marker particularly useful in intermediate-term monitoring and in situation when HbA 1c test is not reliable. We have performed the first multicenter evaluation of the analytical performance of the enzymatic method quantILab Glycated Albumin assay implemented on the most widely used clinical chemistry analyzers (i.e. Abbott Architect C8000, Beckman Coulter AU 480 and 680, Roche Cobas C6000, Siemens ADVIA 2400 and 2400 XPT). The repeatability of the GA measurement (expressed as CV, %) implemented in the participating centers ranged between 0.9% and 1.2%. The within-laboratory CVs ranged between 1.2% and 1.6%. A good alignment between laboratories was found, with correlation coefficients from 0.996 to 0.998. Linearity was confirmed in the range from 7.6 to 84.7%. The new enzymatic method for glycated albumin evaluated by our investigation is suitable for clinical use. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Automated genomic DNA purification options in agricultural applications using MagneSil paramagnetic particles (United States)

    Bitner, Rex M.; Koller, Susan C.


    The automated high throughput purification of genomic DNA form plant materials can be performed using MagneSil paramagnetic particles on the Beckman-Coulter FX, BioMek 2000, and the Tecan Genesis robot. Similar automated methods are available for DNA purifications from animal blood. These methods eliminate organic extractions, lengthy incubations and cumbersome filter plates. The DNA is suitable for applications such as PCR and RAPD analysis. Methods are described for processing traditionally difficult samples such as those containing large amounts of polyphenolics or oils, while still maintaining a high level of DNA purity. The robotic protocols have ben optimized for agricultural applications such as marker assisted breeding, seed-quality testing, and SNP discovery and scoring. In addition to high yield purification of DNA from plant samples or animal blood, the use of Promega's DNA-IQ purification system is also described. This method allows for the purification of a narrow range of DNA regardless of the amount of additional DNA that is present in the initial sample. This simultaneous Isolation and Quantification of DNA allows the DNA to be used directly in applications such as PCR, SNP analysis, and RAPD, without the need for separate quantitation of the DNA.

  3. Normal lymphocyte immunophenotype in an elderly population

    Directory of Open Access Journals (Sweden)

    Sâmia Macedo Queiroz Mota Castellão Tavares


    Full Text Available OBJECTIVE: The aim of this work was to evaluate the lymphocyte immunophenotype in an elderly population.METHODS: This study enrolled 35 over 60-year-old volunteers and a control group composed of 35 young adults. The study included elderly without diseases that might affect the functioning of the immune system. These individuals were consulted by doctors and after a physical examination, laboratory tests were performed using a Beckman Coulter (r flow cytometer. The GraphPad Prism computer program was employed for statistical analysis with the level of significance being set for p-values <0.05.RESULTS: There is a statistically significant reduction in the number of lymphocytes (CD8 +, CD2 + and CD3 + cells in the elderly compared to young adults. These low rates are explained by changes attributed to aging and may be partly responsible for the reduction in the cellular immune response, lower proliferative activity and the low cytotoxicity of lymphocytes.CONCLUSION: These parameters showed greater impairment of adaptive immunity in the elderly population and can therefore explain the greater fragility of the aged body to developing diseases.

  4. Ultrasonic sonoelastography of scrotum in the diagnosis of male fertility

    Directory of Open Access Journals (Sweden)

    O. B. Zhukov


    Full Text Available Objective: to determine the possibility of using detect the scrotum in assessing reproductive function of men.Materials and methods. The study was conducted in men aged 20–44 years. The first group of men was 25 patients with secretory infertility. The second group included 25 male infertility varicocele. The control group presents 12 men in the age range 22–31 year with the implementation of the reproductive function of 1 or more children. The study was performed by ultrasonic scanner expert class Acuson S2000 manufacture of the company Siemens, was used microwave sensor 18L6 HD. Sex hormones were studied on immunochemiluminescence analyzer Access 2 production Beckman Coulter (USA. Obtaining and study of ejaculate was carried out according to Recommendations of the WHO (5th edition.Results. The application of research shear transverse wave Vs men with secretory infertility and varicocele is accompanied by the increase of its value to 1.5–1.6 m/s and is characterized by 35–45 % from similar parameters of reproductive healthy men. In half of the cases studies such an increase in performance and decrease in the area of mapping the so-called “soft” areas of the epididymis is accompanied by a decrease in the concentration and actively motile sperm. The use of such method of instrumental diagnostics will allow at the stage of preliminary ultrasound screening to suspect a violation of the reproductive capacity of men.

  5. The pH of chemistry assays plays an important role in monoclonal immunoglobulin interferences. (United States)

    Alberti, Michael O; Drake, Thomas A; Song, Lu


    Immunoglobulin paraproteins can interfere with multiple chemistry assays. We want to investigate the mechanisms of immunoglobulin interference. Serum samples containing paraproteins from the index patient and eight additional patients were used to investigate the interference with the creatinine and total protein assays on the Beckman Coulter AU5400/2700 analyzer, and to determine the effects of pH and ionic strength on the precipitation of different immunoglobulins in these patient samples. The paraprotein interference with the creatinine and total protein assays was caused by the precipitation of IgM paraprotein in the index patient's samples under alkaline assay conditions. At extremely high pH (12-13) and extremely low pH (1-2) and low ionic strength, paraprotein formed large aggregates in samples from the index patient but not from other patients. The pH and ionic strength are the key factors that contribute to protein aggregation and precipitation which interfere with the creatinine and total protein measurements on AU5400/2700. The different amino acid sequence of each monoclonal paraprotein will determine the pH and ionic strength at which the paraprotein will precipitate.

  6. Journal of Computational Electronics: Proceedings of the International Workshop on Computational Electronics (8th) (IWCE-8), Beckman Institute, University of Illinois, 15-18 October 2001. Volume 1, Issue 1-2 (United States)


    Investigaci6n 48 Oriols through project BFM2000-0353 and by a grant of the Landauer R. 1989. Physica D. 38: 226. Pr cqramna cientifico dle la OTAN...that in the AlSb/InAs and the InAs/GaSb confining inter- will not be dealt with in this article . Our treatment does faces. When the electrons move in the...journals. with the articles . PROCESS FOR SUBMISSION 3. Provide a separate double-spaced sheet listing all footnotes, beginning with "Affiliation of author

  7. A new database sub-system for grain-size analysis (United States)

    Suckow, Axel


    Detailed grain-size analyses of large depth profiles for palaeoclimate studies create large amounts of data. For instance (Novothny et al., 2011) presented a depth profile of grain-size analyses with 2 cm resolution and a total depth of more than 15 m, where each sample was measured with 5 repetitions on a Beckman Coulter LS13320 with 116 channels. This adds up to a total of more than four million numbers. Such amounts of data are not easily post-processed by spreadsheets or standard software; also MS Access databases would face serious performance problems. The poster describes a database sub-system dedicated to grain-size analyses. It expands the LabData database and laboratory management system published by Suckow and Dumke (2001). This compatibility with a very flexible database system provides ease to import the grain-size data, as well as the overall infrastructure of also storing geographic context and the ability to organize content like comprising several samples into one set or project. It also allows easy export and direct plot generation of final data in MS Excel. The sub-system allows automated import of raw data from the Beckman Coulter LS13320 Laser Diffraction Particle Size Analyzer. During post processing MS Excel is used as a data display, but no number crunching is implemented in Excel. Raw grain size spectra can be exported and controlled as Number- Surface- and Volume-fractions, while single spectra can be locked for further post-processing. From the spectra the usual statistical values (i.e. mean, median) can be computed as well as fractions larger than a grain size, smaller than a grain size, fractions between any two grain sizes or any ratio of such values. These deduced values can be easily exported into Excel for one or more depth profiles. However, such a reprocessing for large amounts of data also allows new display possibilities: normally depth profiles of grain-size data are displayed only with summarized parameters like the clay

  8. Detection of an IncA/C plasmid encoding VIM-4 and CMY-4 β-lactamases in Klebsiella oxytoca and Citrobacter koseri from an inpatient in a cardiac rehabilitation unit. (United States)

    Caltagirone, Mariasofia; Bitar, Ibrahim; Piazza, Aurora; Spalla, Melissa; Nucleo, Elisabetta; Navarra, Antonella; Migliavacca, Roberta


    A 62-year-old patient was transferred to the cardiac rehabilitation unit of the I.R.C.C.S. Fondazione S. Maugeri after undergoing a heart transplantation at the Acute Care Hospital I.R.C.C.S. S. Matteo of Pavia. On 1 August 2013 and during hospitalization in the rehabilitation unit, Klebsiella oxytoca and Citrobacter koseri clinical isolates were simultaneously recovered from the patient's preputial swab. Both the K. oxytoca and C. koseri strains were carbapenem- resistant by MicroScan System (Beckman Coulter). Carbapenem-resistant K. pneumoniae had previously been reported in the same rehabilitation facility. The aim of the study was to identify the carbapenem resistance mechanisms among the enterobacterial species recovered. Phenotypic screening tests useful to detect the β-lactamases/carbapenemases were performed. Carbapenem MICs were obtained by Etest. AmpC and MBL encoding genes were identified by PCR and sequencing. Conjugation assays and plasmid characterization were performed. Both of the K. oxytoca and C. koseri isolates were multi drug resistant, showing resistance to amoxicillin-clavulanic acid, three generation cephalosporins, ertapenem (K. oxytoca MIC, >32 mg/L; C. koseri MIC, 4 mg/L), imipenem (K. oxytoca MIC, 4 mg/L; C. koseri MIC, 12 mg/L), thrimethoprim sulphamethoxazole and gentamicin. Susceptibility was retained to fluoroquinolones, colistin and tigecycline. Molecular characterization confirmed the co-presence of blaCMY-4 and blaVIM-4 determinants in a 150 Kb transferable plasmid of IncA/C group. This case is the first detection in Italy of the K. oxytoca and C. koseri clinical isolates co-producing the CMY-4 and VIM-4 enzymes.

  9. The Effect of Increasing Doses of Saw Palmetto Fruit Extract on Serum PSA Levels: Analysis of the CAMUS Randomized Trial (United States)

    Andriole, Gerald L.; McCullum-Hill, Christie; Sandhu, Gurdarshan S.; Crawford, E. David; Barry, Michael J.; Cantor, Alan


    Purpose Saw palmetto extracts are used for treating lower urinary tract symptoms in men despite level I evidence concluding that saw palmetto was ineffective in reducing lower urinary symptoms. We sought to determine whether higher doses of saw palmetto as studied in CAMUS affect serum PSA levels. Materials and Methods The CAMUS trial was a randomized, placebo-controlled double blind multi-centered North American trial conducted between June 5, 2008 and October 10, 2012 in which 369 men >45 years of age with AUA symptom score ≥ 8 and ≤ 24 were randomly assigned to placebo or dose escalation saw palmetto, which consisted of 320mg for first 24 weeks to 640mg for next 24 weeks to 960mg for last 24 weeks of this 72 week trial. Serum PSA levels (Beckman-Coulter) were obtained at baseline and at weeks 24, 48 and 72 and were compared between treatment groups using the pooled t and Fisher's exact tests. Results Serum PSA levels were similar at baseline for the placebo (1.93 ± 1.59 ng/ml) and saw palmetto groups (2.20 ± 1.95, p = 0.16). Changes in PSA levels over the course of the study were similar: placebo group mean change 0.16 ± 1.08 ng/ml and saw palmetto group mean change 0.23 ± 0.83 ng/ml (p value 0.50). Additionally, no differential effect on serum PSA levels was observed between treatment arms when groups were stratified by baseline PSA values. Conclusions Saw palmetto extract does not affect serum PSA levels more than placebo even at relatively high doses. PMID:23253958

  10. Comparison study of the rates of manual peripheral blood smear review from 3 automated hematology analyzers, Unicel DxH 800, ADVIA 2120i, and XE 2100, using international consensus group guidelines. (United States)

    Kim, Sue Jung; Kim, Yoonjung; Shin, Saeam; Song, Jaewoo; Choi, Jong Rak


    In the clinical laboratory, it is important both to reduce the number of peripheral blood slide reviews to save time and money and to avoid reporting false results. To determine differences in the slide review rates of 3 widely used automated hematologic analyzers, the Unicel DxH 800 (Beckman Coulter Inc, Fullerton, California), ADVIA 2120i (Siemens Diagnostics, Tarrytown, New York), and XE 2100 (Sysmex, Kobe, Japan), using International Consensus Group for Hematology Review guidelines. A total of 1485 samples were tested, and 300 were manually reviewed. Slide review rates, sensitivity, specificity, and false-positive and false-negative rates were estimated using consensus group rules and compared using χ(2) tests, Fisher exact tests, or generalized estimating equations. Unicel DxH 800, ADVIA 2120i, and XE 2100 showed 22.8%, 20.2%, and 28.6% slide review rates; 14.3%, 14.3%, and 9.7% false-negative rates; and 13.7, 11.3%, and 17.3% false-positive rates, respectively. All analyzers showed significantly higher false-negative rates than that of the consensus group (2.9%). False-negative rates were higher than the recommended levels. Among 3 automated hematologic analyzers, XE 2100 showed the highest rate of slide review. Because the present study clearly shows that the slide review rates have distinct characteristics among the studied analyzers, each individual laboratory should consider selecting the most appropriate analyzer according to clinical characteristics. Analyzers with high sensitivity may be advantageous in outpatient settings for screening patients, whereas analyzers with high specificity may be beneficial in inpatient settings for efficient patient care.

  11. Iron status and its determinants in a nationally representative sample of pregnant women. (United States)

    Vandevijvere, Stefanie; Amsalkhir, Sihame; Van Oyen, Herman; Egli, Ines; Ines, Egli; Moreno-Reyes, Rodrigo


    Iron-deficiency anemia is associated with adverse neonatal health outcomes. Iron status and its determinants were assessed in a representative sample of Belgian pregnant women. Blood samples were collected and a questionnaire was completed face-to-face. Hemoglobin (Hb) and mean cell volume were measured using a Beckman Coulter Hematology Analyzer and serum ferritin (SF) and transferrin receptor (sTfr) concentrations by immunoassay. In total, 55 obstetric clinics and 1,311 pregnant women were included. Approximately 40% of third-trimester and 6% of first-trimester women had SF levels less than 15 μg/L. Approximately 21% of third-trimester and 4% of first-trimester women had anemia (Hb 8.5 mg/L). The median body iron stores were 8.1 mg/kg among first-trimester women, but only 3.6 mg/kg among third-trimester women. SF levels were significantly positively associated with age and education level, and were higher among nulliparous women and lower among North-African women. sTfr concentrations were significantly negatively associated with age and were lower among smokers, nulliparous women, and women who planned their pregnancy. Despite the fact that two thirds of Belgian pregnant women took iron-containing supplements, iron deficiency and iron-deficiency anemia were frequent in third-trimester women. The World Health Organization regards this as a moderate public health problem. National iron supplementation guidelines are needed in Belgium to optimize iron status during pregnancy. Copyright © 2013 Academy of Nutrition and Dietetics. Published by Elsevier Inc. All rights reserved.

  12. Competition between autotrophic and heterotrophic microbial plankton for inorganic nutrients induced by variability in estuarine biophysicochemical conditions (United States)

    Williams, A.; Quigg, A.


    Competition for inorganic nutrients between autotrophic and heterotrophic fractions of microbial plankton (0.2-20μm) was investigated at two stations in a sub-tropical estuary, Galveston Bay, Texas. Competition potential between these groups is enhanced because individuals are similar in size, reducing variability among their nutrient uptake efficiencies. Further, in estuaries, allochthonous supplements to autochthonous carbon may satisfy heterotrophic requirements, allowing alternative factors to limit abundance. The relative abundance of autotrophs and heterotrophs stained with SYBR Green I and enumerated on a Beckman Coulter Gallios flow cytometer were evaluated monthly during a year-long study. Shifts in the relative in situ abundance were significantly related to temperature, dissolved inorganic nitrogen (DIN), phosphorous (Pi), and total organic carbon (TOC) concentrations revealing opposing gradients of limitation by different abiotic factors. In corresponding in vitro nutrient enrichment bioassays the relative contribution of autotrophic or heterotrophic microbial plankton to significant enrichment responses varied. Only during macro- (>20μm) phytoplankton blooms do autotrophic microbial plankton respond to nutrient enrichment. Contrastingly, the heterotrophic microbial plankton responded to nutrient enrichment primarily when temperature limitation was alleviated. Therefore, the potential for autotrophic and heterotrophic microbial plankton competition for limiting nutrients is highest when autotrophic microbial plankton are also competing with larger phytoplankton during bloom events. Based on this evidence, we hypothesize that the autotrophic microbial fraction has a competitive advantage over the heterotrophs for inorganic nutrients in Galveston Bay. The observed microbial competition during estuarine phytoplankton blooms may have important consequences on biogeochemical processes including carbon and nutrient cycling.

  13. Iterative Outlier Removal: A Method for Identifying Outliers in Laboratory Recalibration Studies. (United States)

    Parrinello, Christina M; Grams, Morgan E; Sang, Yingying; Couper, David; Wruck, Lisa M; Li, Danni; Eckfeldt, John H; Selvin, Elizabeth; Coresh, Josef


    Extreme values that arise for any reason, including those through nonlaboratory measurement procedure-related processes (inadequate mixing, evaporation, mislabeling), lead to outliers and inflate errors in recalibration studies. We present an approach termed iterative outlier removal (IOR) for identifying such outliers. We previously identified substantial laboratory drift in uric acid measurements in the Atherosclerosis Risk in Communities (ARIC) Study over time. Serum uric acid was originally measured in 1990-1992 on a Coulter DACOS instrument using an uricase-based measurement procedure. To recalibrate previous measured concentrations to a newer enzymatic colorimetric measurement procedure, uric acid was remeasured in 200 participants from stored plasma in 2011-2013 on a Beckman Olympus 480 autoanalyzer. To conduct IOR, we excluded data points >3 SDs from the mean difference. We continued this process using the resulting data until no outliers remained. IOR detected more outliers and yielded greater precision in simulation. The original mean difference (SD) in uric acid was 1.25 (0.62) mg/dL. After 4 iterations, 9 outliers were excluded, and the mean difference (SD) was 1.23 (0.45) mg/dL. Conducting only one round of outlier removal (standard approach) would have excluded 4 outliers [mean difference (SD) = 1.22 (0.51) mg/dL]. Applying the recalibration (derived from Deming regression) from each approach to the original measurements, the prevalence of hyperuricemia (>7 mg/dL) was 28.5% before IOR and 8.5% after IOR. IOR is a useful method for removal of extreme outliers irrelevant to recalibrating laboratory measurements, and identifies more extraneous outliers than the standard approach. © 2016 American Association for Clinical Chemistry.

  14. Effects of Weight Reduction After Sleeve Gastrectomy on Metabolic Variables in Saudi Obese Subjects in Aseer Province of Kingdom of Saudi Arabia. (United States)

    Bawahab, Mohammed A; Assiri, Abdullah S; Maksoud, Walid Abdel; Patel, Ayyub; Kadoumi, Osama; Zaman, Gaffar Sarwar; Alessih, Riyad Mohammed Khalil; Haider, Syed Saleem


    The objectives of this study were to investigate the occurrence of oxidative stress, status of protective antioxidants enzymes, inflammatory biomarkers, and some metabolic health variables in the blood and to compare the results between those of the normal controls and obese patients submitted to sleeve gastrectomy-induced weight loss over a 1-year follow-up period. A prospective study was conducted in Aseer Central Hospital and Abha Private Hospital in the Kingdom of Saudi Arabia from January 2012 to January 2013 on 50 normal (BMI = 22-25 kg/m 2 ) control subjects and 50 obese (BMI = 45-50 kg/m 2 ) patients. A subset of 20 men and 80 women patients, aged 20-45 years, was included. The systemic blood cell counts were determined by Beckman Coulter UniCel analyzer. The occurrence of oxidative stress, the status of antioxidant enzyme system in the blood, levels of serum hepatic enzymes, cardiovascular risk factors, and serum sodium, potassium, copper, and zinc levels were determined by spectrophotometric procedures. The concentration of TSH and T 4 were analyzed by Siemens Immunoassay System. Group 1 (Obese: preoperative) This group compared with the normal controls exhibited significant (p copper and zinc levels were significantly (p phosphate dehydrogenase (G6PDH), and vitamin C, were remarkably (p potassium, copper, zinc, and TSH levels were significantly (p < 0.001) decreased. Obesity is a chronic disease of multifactorial origin and resulted in perturbations of whole body metabolism in this study. It is thus likely that this imbalance was associated with an inhibition in protective antioxidants and occurrence of oxidative stress. The staging concept of sleeve gastrectomy is a safe and effective approach with remarkable efficacy in sustaining weight loss and bringing back normal metabolism of variables in tissues over a 1-year follow-up period.

  15. The role of biochemical variations and genotype testing in determining the virological response of patients infected with hepatitis C virus

    Directory of Open Access Journals (Sweden)

    Abid Shoukat


    Full Text Available Background: In hepatitis C virus (HCV, infection viral and IL28B genotype along with many clinical and biochemical factors can influence response rates to pegylated interferon plus ribavirin (Peg-IFN-a/R therapy and progression to chronic hepatitis C (CHC. Aims: The present study was conducted to determine the effect of biochemical and risk factors on treatment outcome in CHC patients in relation to their viral and host genotype. Settings and Design: The present study was a prospective Pe- IFN efficacy study consisting of Peg-IFN-a/R therapy for 24–48 weeks including 250 HCV infected patients. Materials and Methods: Biochemical parameters were determined by Beckman Coulter AU680 automated analyzer. HCV and Interleukin 28B (IL28B genotyping were carried out by polymerase chain reaction-restriction fragment length polymorphism and viral load was determined by quantitative real-time PCR. Results: Wild outnumbered the variant genotypes in rs 12979860, rs 12980275, and rs 8099917 SNP of IL28B gene. Sustained virological response (SVR SVR and viral genotype were significantly associated with age, hepatic steatosis, low-grade varices, and serum aspartate transaminase levels (at the end of treatment (P < 0.05. In addition, SVR was significantly influenced by body mass index (BMI, insulin resistance, serum low-density lipoprotein , and ferritin levels (P < 0.05. Viral genotype 1 infected patients had higher serum cholesterol and triglyceride levels (P < 0.05. Conclusions: Although the IL28B sequence variation is the major factor that can influence response rates to antiviral therapy, viral and biochemical factors also have a definite role to play in the diagnosis, etiology, and treatment outcome in HCV-infected patients.

  16. Emergency medical technician-performed point-of-care blood analysis using the capillary blood obtained from skin puncture. (United States)

    Kim, Changsun; Kim, Hansol


    Comparing a point-of-care (POC) test using the capillary blood obtained from skin puncture with conventional laboratory tests. In this study, which was conducted at the emergency department of a tertiary care hospital in April-July 2017, 232 patients were enrolled, and three types of blood samples (capillary blood from skin puncture, arterial and venous blood from blood vessel puncture) were simultaneously collected. Each blood sample was analyzed using a POC analyzer (epoc® system, USA), an arterial blood gas analyzer (pHOx®Ultra, Nova biomedical, USA) and venous blood analyzers (AU5800, DxH2401, Beckman Coulter, USA). Twelve parameters were compared between the epoc and reference analyzers, with an equivalence test, Bland-Altman plot analysis and linear regression employed to show the agreement or correlation between the two methods. The pH, HCO 3 , Ca 2+ , Na + , K + , Cl - , glucose, Hb and Hct measured by the epoc were equivalent to the reference values (95% confidence interval of mean difference within the range of the agreement target) with clinically inconsequential mean differences and narrow limits of agreement. All of them, except pH, had clinically acceptable agreements between the two methods (results within target value ≥80%). Of the remaining three parameters (pCO 2, pO 2 and lactate), the epoc pCO 2 and lactate values were highly correlated with the reference device values, whereas pO 2 was not. (pCO 2 : R 2 =0.824, y=-1.411+0.877·x; lactate: R 2 =0.902, y=-0.544+0.966·x; pO 2 : R 2 =0.037, y=61.6+0.431·x). Most parameters, except only pO 2 , measured by the epoc were equivalent to or correlated with those from the reference method. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. White blood cells and subtypes in HFE p.C282Y and wild-type homozygotes in the Hemochromatosis and Iron Overload Screening Study. (United States)

    Barton, James C; Barton, J Clayborn; Acton, Ronald T


    The major histocompatibility complex is linked to white blood cell (WBC) and lymphocyte counts in subjects unselected for HFE genotypes. We compared age, sex, body mass index, total WBC and subtypes (neutrophils, lymphocytes, monocytes, eosinophils, basophils) (Beckman Coulter® Gen-S), transferrin saturation, and serum ferritin of HFE p.C282Y and wild-type (p.C282Y, p.H63D negative) homozygotes without acquired conditions that influence WBC counts. We performed regressions on WBC and subtypes. There were 161 p.C282Y homozygotes (45.3% men) and 221 wild-type homozygotes (40.3% men). Mean WBC of men and women and between HFE genotypes were similar. Mean lymphocytes were higher in male p.C282Y homozygotes: 1.6×10 9 /L [95% confidence interval: 1.5,1.7] vs. 1.4 [1.3,1.5], p=0.0002. Mean lymphocytes and basophils were higher in female p.C282Y homozygotes: 1.6 [1.5,1.7] vs. 1.4 [1.3,1.5], p=0.0002; and 0.065 [0.059,0.071] vs. 0.052 [0.051,0.054], p=0.0001, respectively. Transferrin saturation was associated with neutrophils (negative; p=0.0163). Age was associated with lymphocytes (negative; p=0.0003) and monocytes (positive; p<0.0001). Regressions on lymphocytes and basophils revealed positive associations with p.C282Y homozygosity (p=0.0043 and 0.0003, respectively). There were significant positive associations of neutrophils, lymphocytes, monocytes, and eosinophils. We conclude that HFE p.C282Y homozygosity is significantly associated with lymphocyte and basophil counts. Copyright © 2016 Elsevier Inc. All rights reserved.

  18. European Multicenter Study on Analytical Performance of Veris HIV-1 Assay. (United States)

    Braun, Patrick; Delgado, Rafael; Drago, Monica; Fanti, Diana; Fleury, Hervé; Hofmann, Jörg; Izopet, Jacques; Kalus, Ulrich; Lombardi, Alessandra; Marcos, Maria Angeles; Mileto, Davide; Sauné, Karine; O'Shea, Siobhan; Pérez-Rivilla, Alfredo; Ramble, John; Trimoulet, Pascale; Vila, Jordi; Whittaker, Duncan; Artus, Alain; Rhodes, Daniel W


    The analytical performance of the Veris HIV-1 assay for use on the new, fully automated Beckman Coulter DxN Veris molecular diagnostics system was evaluated at 10 European virology laboratories. The precision, analytical sensitivity, performance with negative samples, linearity, and performance with HIV-1 groups/subtypes were evaluated. The precision for the 1-ml assay showed a standard deviation (SD) of 0.14 log 10 copies/ml or less and a coefficient of variation (CV) of ≤6.1% for each level tested. The 0.175-ml assay showed an SD of 0.17 log 10 copies/ml or less and a CV of ≤5.2% for each level tested. The analytical sensitivities determined by probit analysis were 19.3 copies/ml for the 1-ml assay and 126 copies/ml for the 0.175-ml assay. The performance with 1,357 negative samples demonstrated 99.2% with not detected results. Linearity using patient samples was shown from 1.54 to 6.93 log 10 copies/ml. The assay performed well, detecting and showing linearity with all HIV-1 genotypes tested. The Veris HIV-1 assay demonstrated analytical performance comparable to that of currently marketed HIV-1 assays. (DxN Veris products are Conformité Européenne [CE]-marked in vitro diagnostic products. The DxN Veris product line has not been submitted to the U.S. FDA and is not available in the U.S. market. The DxN Veris molecular diagnostics system is also known as the Veris MDx molecular diagnostics system and the Veris MDx system.). Copyright © 2017 American Society for Microbiology.

  19. Nationwide Multicenter Reference Interval Study for 28 Common Biochemical Analytes in China. (United States)

    Xia, Liangyu; Chen, Ming; Liu, Min; Tao, Zhihua; Li, Shijun; Wang, Liang; Cheng, Xinqi; Qin, Xuzhen; Han, Jianhua; Li, Pengchang; Hou, Li'an; Yu, Songlin; Ichihara, Kiyoshi; Qiu, Ling


    A nationwide multicenter study was conducted in the China to explore sources of variation of reference values and establish reference intervals for 28 common biochemical analytes, as a part of the International Federation of Clinical Chemistry and Laboratory Medicine, Committee on Reference Intervals and Decision Limits (IFCC/C-RIDL) global study on reference values. A total of 3148 apparently healthy volunteers were recruited in 6 cities covering a wide area in China. Blood samples were tested in 2 central laboratories using Beckman Coulter AU5800 chemistry analyzers. Certified reference materials and value-assigned serum panel were used for standardization of test results. Multiple regression analysis was performed to explore sources of variation. Need for partition of reference intervals was evaluated based on 3-level nested ANOVA. After secondary exclusion using the latent abnormal values exclusion method, reference intervals were derived by a parametric method using the modified Box-Cox formula. Test results of 20 analytes were made traceable to reference measurement procedures. By the ANOVA, significant sex-related and age-related differences were observed in 12 and 12 analytes, respectively. A small regional difference was observed in the results for albumin, glucose, and sodium. Multiple regression analysis revealed BMI-related changes in results of 9 analytes for man and 6 for woman. Reference intervals of 28 analytes were computed with 17 analytes partitioned by sex and/or age. In conclusion, reference intervals of 28 common chemistry analytes applicable to Chinese Han population were established by use of the latest methodology. Reference intervals of 20 analytes traceable to reference measurement procedures can be used as common reference intervals, whereas others can be used as the assay system-specific reference intervals in China.

  20. Age-Specific Normal Reference Range for Serum Anti-Müllerian Hormone in Healthy Chinese Han Women: A nationwide Population-Based Study. (United States)

    Du, Xiaofang; Ding, Ting; Zhang, Hanwang; Zhang, Cuilian; Ma, Wenmin; Zhong, Ying; Qu, Wenyu; Zheng, Jie; Liu, Yi; Li, Zhiying; Huang, Kecheng; Deng, Song; Ma, Lanfang; Yang, Jun; Jiang, Jingjing; Yang, Shuhong; Huang, Jia; Wu, Meng; Fang, Li; Lu, Yunping; Luo, Aiyue; Wang, Shixuan


    The increasing use of anti-Müllerian hormone (AMH) in clinic has raised concerns regarding the reliable reference range for this test. However, the reference range for AMH in normal Chinese female population has not been established. Furthermore, relationship between AMH and other clinical markers such as body mass index (BMI) and antral follicle counts (AFCs) and other sex-related hormones have not been examined in normal population-based women. We aimed to determine the age-specific reference range for serum AMH in healthy Chinese women throughout reproductive age to menopause and to estimate relationship between AMH and other clinical markers in healthy women. In this multicenter and nationwide study, advertisements were used to recruit 2055 women, aged 20 to 55 years, from 6 different regions in China; 1590 (77.37%) women met the inclusion criteria for the reference range population. We measured the baseline serum AMH levels using new Beckman Coulter Gen II assay. Serum concentration of follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), testosterone (T), prolactin (PRL), progesterone (PRG), and AFCs were also determined in the follicular phase. The AMH-Age nomogram and AMH levels of different age-groups and the relationship between AMH and other clinical markers. Serum AMH concentrations declined progressively with age. A quadratic model defined as log (AMH) = (-1.970 + 0.296 × Age - 0.006 × Age(2)) fitted best the decline of AMH with age. The median AMH levels were 6.23, 5.65, 4.55, 3.74, 2.78, and 1.09 ng/mL for the 20 ≤ age women. © The Author(s) 2016.

  1. Female age, serum antimüllerian hormone level, and number of oocytes affect the rate and number of euploid blastocysts in in vitro fertilization/intracytoplasmic sperm injection cycles. (United States)

    La Marca, Antonio; Minasi, Maria Giulia; Sighinolfi, Giovanna; Greco, Pierfrancesco; Argento, Cindy; Grisendi, Valentina; Fiorentino, Francesco; Greco, Ermanno


    To study the relative role of female age and ovarian reserve, measured through serum antimüllerian hormone (AMH) in determining the rate and number of euploid blastocysts in in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycles. Retrospective analysis of cycles performed in 2014-2015. Tertiary referral IVF center. A total of 578 infertile couples undergoing IVF/ICSI and preimplantation genetic screening (PGS) analysis. All embryos were cultured and biopsied at the blastocyst stage. The method involved whole-genome amplification followed by array comparative genome hybridization. Serum AMH was measured by means of the modified Beckman Coulter AMH Gen II assay. The rate and number of euploid blastocysts and their correlation with ovarian reserve and response to stimulation. The mean (±SD) age of patients was 37.6 ± 4.1 years, and the mean number of blastocysts per patient was 3.1 ± 2. The total number of blastocysts available to the analysis was 1,814, and 36% of them were euploid after PGS. Age and serum AMH were significantly and independently related to the rate of euploid blastocysts available for patients. As an effect of the cohort size, the number of mature oocytes positively affected the total number of euploid blastocysts per patient. A strong positive age-independent relationship between AMH level and the rate of euploid blastocysts was found. This confirms that the measurement of ovarian reserve by means of AMH has high relevance when counseling infertile patients. Copyright © 2017 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  2. Evaluation of the highly sensitive Roche thyroglobulin II assay and establishment of a reference limit for thyroglobulin-negative patient samples

    Directory of Open Access Journals (Sweden)

    Dorien M. Rotteveel-de Groot


    Full Text Available Objectives: Thyroglobulin (Tg measurements are used to monitor for residual thyroid tissue in patients with differentiated thyroid cancer (DTC after thyroidectomy and radioiodine ablative therapy. In recent years highly sensitive Tg assays have been developed. In this study the analytical performance of the new Roche Elecsys Tg II assay was evaluated and compared with the well documented Access2 Tg assay (Beckman–Coulter. Design and methods: Analytical performance was examined using various Clinical and Laboratory Standards Institute (CLSI evaluation protocols. Tg negative patient sera were used to establish an upper reference limit (URL for the Elecsys Tg II assay. Results: Non-linearity, drift and carry-over according to CLSI EP10 and EP6 in a measuring range of 0.04–500 ng/mL were non-significant. Total precision according to CLSI EP5 was 10% at a Tg concentration of 0.08 ng/mL. A patient serum comparison performed according to a modified CLSI EP9 protocol showed a significant difference of a factor of approximately 1.4, despite using an identical CRM calibrator. The Elecsys Tg II assay measured Tg with a two-fold higher sensitivity than the Access2 assay. Finally, using human sera without Tg, an URL of 0.05 ng/mL was determined. Conclusions: In our hands the highly sensitive Elecsys Tg II assay shows a good analytical performance and a higher sensitivity compared to the Access2 Tg assay. An URL of 0.05 ng/mL for the Elecsys Tg II assay was determined which may improve the clinical utility of the assay for the detection of residual DTC or disease recurrence. Keywords: Thyroglobulin, Roche Elecsys Tg II assay, validation, reporting limit

  3. Mean corpuscular volume of control red blood cells determines the interpretation of eosin-5'-maleimide (EMA) test result in infants aged less than 6 months. (United States)

    Ciepiela, Olga; Adamowicz-Salach, Anna; Bystrzycka, Weronika; Łukasik, Jan; Kotuła, Iwona


    Eosin-5'-maleimide (EMA) binding test is a flow cytometric test used to detect hereditary spherocytosis (HS). To perform the test sample from patients, 5-6 reference samples of red blood are needed. Our aim was to investigate how the mean corpuscular volume (MCV) of red blood cells influences on the value of fluorescence of bounded EMA dye and how the choice of reference samples affects the test result. EMA test was performed in peripheral blood from 404 individuals, including 31 children suffering from HS. Mean fluorescence channel of EMA-RBCs was measured with Cytomics FC500 flow cytometer. Mean corpuscular volume of RBCs was assessed with LH750 Beckman Coulter. Statistical analysis was performed using Graph Pad Prism. The correlation Spearman coefficient between mean channel of fluorescence of EMA-RBCs and MCV was r = 0.39, p < 0.0001. Interpretation of EMA test depends on MCV of the reference samples. If reference blood samples have lower MCV than the patients MCV, EMA test result might be negative. Due to different MCV values of RBCs in infancy and ca. Three months later, EMA test in neonates might be interpreted falsely negative. Samples from children younger than 3 months old had EMA test result 86.1 ± 11.7 %, whereas same samples that analyzed 4.1 ± 2.1 later had results of 75.4 ± 4.5 %, p < 0.05. Mean fluorescence of EMA-bound RBC depends on RBC's volume. MCV of reference samples affects EMA test results; thus, we recommend selection of reference samples with MCV in range of ±2 fL compared to MCV of patient RBC's.

  4. Detecting paraprotein interference on a direct bilirubin assay by reviewing the photometric reaction data. (United States)

    García-González, Elena; Aramendía, Maite; González-Tarancón, Ricardo; Romero-Sánchez, Naiara; Rello, Luis


    The direct bilirubin (D-Bil) assay on the AU Beckman Coulter instrumentation can be interfered by paraproteins, which may result in spurious D-Bil results. In a previous work, we took advantage of this fact to detect this interference, thus helping with the identification of patients with unsuspected monoclonal gammopathies. In this work, we investigate the possibility to detect interference based on the review of the photometric reactions, regardless of the D-Bil result. The D-Bil assay was carried out in a set of 2164 samples. It included a group of 164 samples with paraproteins (67 of which caused interference on the assay), as well as different groups of samples for which high absorbance background readings could also be expected (i.e. hemolyzed, lipemic, or icteric samples). Photometric reaction data were reviewed and receiver operating characteristics (ROC) curves were used to establish a cut-off for absorbance that best discriminates interference. The best cut-off was 0.0100 for the absorbance at the first photometric point of the complementary wavelength in the blank cuvette. Once the optimal cut-off for probable interference was selected, all samples analyzed in our laboratory that provided absorbance values above this cut-off were further investigated to try to discover paraproteins. During a period of 6 months, we detected 44 samples containing paraproteins, five of which belonged to patients with non-diagnosed monoclonal gammopathies. Review of the photometric reaction data permits the systematic detection of paraprotein interference on the D-Bil AU assay, even for samples for which reasonable results are obtained.

  5. Early diagnosis of myocardial infarction using absolute and relative changes in cardiac troponin concentrations. (United States)

    Irfan, Affan; Reichlin, Tobias; Twerenbold, Raphael; Meister, Marc; Moehring, Berit; Wildi, Karin; Bassetti, Stefano; Zellweger, Christa; Gimenez, Maria Rubini; Hoeller, Rebeca; Murray, Karsten; Sou, Seoung Mann; Mueller, Mira; Mosimann, Tamina; Reiter, Miriam; Haaf, Philip; Ziller, Ronny; Freidank, Heike; Osswald, Stefan; Mueller, Christian


    Absolute changes in high-sensitivity cardiac troponin T (hs-cTnT) seem to have higher diagnostic accuracy in the early diagnosis of acute myocardial infarction compared with relative changes. It is unknown whether the same applies to high-sensitivity cardiac troponin I (hs-cTnI) assays and whether the combination of absolute and relative change might further increase accuracy. In a prospective, international multicenter study, high-sensitivity cardiac troponin (hs-cTn) was measured with 3 novel assays (hs-cTnT, Roche Diagnostics Corp, Indianapolis, Ind; hs-cTnI, Beckman Coulter Inc, Brea, Calif; hs-cTnI, Siemens, Munich, Germany) in a blinded fashion at presentation and after 1 and 2 hours in a blinded fashion in 830 unselected patients with suspected acute myocardial infarction. The final diagnosis was adjudicated by 2 independent cardiologists. The area under the receiver operating characteristic curve for diagnosing acute myocardial infarction was significantly higher for 1- and 2-hour absolute versus relative hs-cTn changes for all 3 assays (P Siemens, 0.96 [95% CI, 0.93-0.99]) were high and provided some benefit compared with the use of absolute change alone for hs-cTnT, but not for the hs-cTnI assays. Reclassification analysis confirmed the superiority of absolute changes versus relative changes. Absolute changes seem to be the preferred metrics for both hs-cTnT and hs-cTnI in the early diagnosis of acute myocardial infarction. The combination of absolute and relative changes provides a small added value for hs-cTnT, but not for hs-cTnI. Copyright © 2013 Elsevier Inc. All rights reserved.

  6. Improved differential diagnosis of anemia of chronic disease and iron deficiency anemia: a prospective multicenter evaluation of soluble transferrin receptor and the sTfR/log ferritin index. (United States)

    Skikne, Barry S; Punnonen, Kari; Caldron, Paul H; Bennett, Michael T; Rehu, Mari; Gasior, Gail H; Chamberlin, Janna S; Sullivan, Linda A; Bray, Kurtis R; Southwick, Paula C


    Anemia of chronic disease (ACD) and iron deficiency anemia (IDA) are the most prevalent forms of anemia and often occur concurrently. Standard tests of iron status used in differential diagnosis are affected by inflammation, hindering clinical interpretation. In contrast, soluble transferrin receptor (sTfR) indicates iron deficiency and is unaffected by inflammation. Objectives of this prospective multicenter clinical trial were to evaluate and compare the diagnostic accuracy of sTfR and the sTfR/log ferritin index (sTfR Index) for differential diagnosis using the automated Access(®) sTfR assay (Beckman Coulter) and sTfR Index. We consecutively enrolled 145 anemic patients with common disorders associated with IDA and ACD. Subjects with IDA or ACD + IDA had significantly higher sTfR and sTfR Index values than subjects with ACD (P < 0.0001). ROC curves produced the following cutoffs for sTfR: 21 nmol/L (or 1.55 mg/L), and the sTfR Index: 14 (using nmol/L) (or 1.03 using mg/L). The sTfR Index was superior to sTfR (AUC 0.87 vs. 0.74, P < 0.0001). Use of all three parameters in combination more than doubled the detection of IDA, from 41% (ferritin alone) to 92% (ferritin, sTfR, sTfR Index). Use of sTfR and the sTfR Index improves detection of IDA, particularly in situations where routine markers provide equivocal results. Findings demonstrate a significant advantage in the simultaneous determination of ferritin, sTfR and sTfR Index. Obtaining a ferritin level alone may delay diagnosis of combined IDA and ACD. Copyright © 2011 Wiley-Liss, Inc.

  7. Relationship of chronic histologic prostatic inflammation in biopsy specimens with serum isoform [-2]proPSA (p2PSA), %p2PSA, and prostate health index in men with a total prostate-specific antigen of 4-10 ng/ml and normal digital rectal examination. (United States)

    Lazzeri, Massimo; Abrate, Alberto; Lughezzani, Giovanni; Gadda, Giulio Maria; Freschi, Massimo; Mistretta, Francesco; Lista, Giuliana; Fossati, Nicola; Larcher, Alessandro; Kinzikeeva, Ella; Buffi, Nicolòmaria; Dell'Acqua, Vincenzo; Bini, Vittorio; Montorsi, Francesco; Guazzoni, Giorgio


    To investigate the relationship between serum [-2]proPSA (p2PSA) and derivatives with chronic histologic prostatic inflammation (CHPI) in men undergoing prostate biopsy for suspected prostate cancer (PCa). This nested case-control study resulted from an observational prospective trial for the definition of sensibility, specificity, and accuracy of p2PSA, %p2PSA, and Beckman Coulter Prostate Health Index (PHI), in men undergoing prostate biopsy, with a total prostate-specific antigen (PSA) of 4-10 ng/mL and normal digital rectal examination. CHPI was the outcome of interest and defined as the presence of moderate to large infiltration of lymphomononuclear cells with interstitial and/or glandular disruption in absence of PCa. p2PSA, %p2PSA, and PHI were considered the index tests and compared with the established biomarker reference standard tests: tPSA, fPSA, %fPSA. Of 267 patients subjected to prostate biopsy, 73 (27.3%) patients were diagnosed with CHPI. Comparing CHPI with PCa patients, %p2PSA and PHI were found to be significantly lower, whereas fPSA and %fPSA were significantly higher. %p2PSA and PHI were the most accurate predictors of CHPI at biopsy, significantly outperforming tPSA, fPSA, and %fPSA. On the contrary, no significant differences were found in PSA, p2PSA, and derivatives between CHPI and benign prostatic hyperplasia (BPH) patients. Our findings showed that p2PSA, %p2PSA, and PHI values might discriminate PCa from CHPI or BPH, but not CHPI from BPH, in men with a total PSA 4-10 ng/mL and normal digital rectal examination. p2PSA isoform and its derivatives could be useful in clinical decision making to avoid unnecessary biopsies in patients with CHPI and elevated tPSA value. Copyright © 2014 Elsevier Inc. All rights reserved.

  8. Performance of serum prostate-specific antigen isoform [-2]proPSA (p2PSA) and the prostate health index (PHI) in a Chinese hospital-based biopsy population. (United States)

    Na, Rong; Ye, Dingwei; Liu, Fang; Chen, Haitao; Qi, Jun; Wu, Yishuo; Zhang, Guiming; Wang, Meilin; Wang, Wenying; Sun, Jielin; Yu, Guopeng; Zhu, Yao; Ren, Shancheng; Zheng, S Lilly; Jiang, Haowen; Sun, Yinghao; Ding, Qiang; Xu, Jianfeng


    The use of serum [-2]proPSA (p2PSA) and its derivative, the prostate health index (PHI), in detecting prostate cancer (PCa) have been consistently shown to have better performance than total prostate-specific antigen (tPSA) in discriminating biopsy outcomes in western countries. However, little is known about their performance in Chinese men. Our objective is to test the performance of p2PSA and PHI and their added value to tPSA in discriminating biopsy outcomes in Chinese men. Consecutive patients who underwent prostate biopsy in three tertiary hospitals in Shanghai, China during 2012-2013 were recruited. Serum tPSA, free PSA (fPSA), and p2PSA were measured centrally using Beckman Coulter's DxI 800 Immunoassay System. The primary outcome is PCa and the secondary outcome is high-grade PCa (Gleason Score of 4 + 3 or worse). Discriminative performance was assessed using the area under the receiver operating characteristic curve (AUC), detection rate and Decision Curve Analysis (DCA). Among 636 patients who underwent prostate biopsy, PHI was a significant predictor of biopsy outcomes, independent of other clinical variables. The AUC in discriminating PCa from non-PCa was consistently higher for PHI than tPSA in the entire cohort (0.88 vs. 0.81) as well as in patients with tPSA at 2-10 ng/ml (0.73 vs. 0.53), at 10.1-20 ng/ml (0.81 vs. 0.58), and at tPSA >20 ng/ml (0.90 vs. 0.80). The differences were statistically significant in all comparisons, P prostate biopsy in China. © 2014 Wiley Periodicals, Inc.

  9. A European multicientre study on the comparison of HBV viral loads between VERIS HBV assay and Roche COBAS® TAQMAN® HBV test, Abbott RealTime HBV assay, Siemens VERSANT HBV assay, and Qiagen artus HBV RG kit. (United States)

    Braun, Patrick; Delgado, Rafael; Drago, Monica; Fanti, Diana; Fleury, Hervé; Izopet, Jacques; Lombardi, Alessandra; Marcos, MaAngeles; Sauné, Karine; O'Shea, Siobhan; Pérez-Rivilla, Alfredo; Ramble, John; Trimoulet, Pascale; Vila, Jordi; Whittaker, Duncan; Artus, Alain; Rhodes, Daniel


    Hepatitis B viral load testing is essential to treatment and monitoring decisions in patients with chronic Hepatitis B. Beckman Coulter has developed the VERIS HBV Assay (Veris) for use on the fully automated DxN VERIS Molecular Diagnostics System. 1 OBJECTIVES: To evaluate the clinical performance of the Veris HBV Assay at multiple EU laboratories STUDY DESIGN: Method comparison was performed with a total of 344 plasma specimens from HBV infected patients tested with Veris and COBAS ® TaqMan ® HBV Test (Cobas), 207 specimens tested with Veris and RealTime HBV Assay (RealTime), 86 specimens tested with Veris and VERSANT ® HBV Assay (Versant), and 74 specimens tested with Veris and artus ® HBV RG PCR kit (artus). Bland-Altman analysis showed average bias of -0.46 log 10 IU/mL between Veris and Cobas, -0.46 log 10 IU/mL between Veris and RealTime, -0.36 log 10 IU/mL between Veris and Versant, and -0.12 log 10 IU/mL between Veris and artus. Bias was consistent across the assay range. Patient monitoring results using Veris demonstrated similar viral load trends over time to Cobas, RealTime, and artus. The VERIS HBV Assay demonstrated comparable clinical performance, with varying degrees of negative bias, compared to other currently marketed assays for HBV DNA monitoring. This negative bias should be taken into consideration if switching monitoring methods to Veris. Copyright © 2017 Elsevier B.V. All rights reserved.

  10. A European multicientre study on the comparison of HIV-1 viral loads between VERIS HIV-1 Assay and Roche COBAS® TAQMAN® HIV-1 test, Abbott RealTime HIV-1 Assay, and Siemens VERSANT HIV-1 Assay. (United States)

    Braun, Patrick; Delgado, Rafael; Drago, Monica; Fanti, Diana; Fleury, Hervé; Hofmann, Jörg; Izopet, Jacques; Kühn, Sebastian; Lombardi, Alessandra; Mancon, Alessandro; Marcos, Mª Angeles; Mileto, Davide; Sauné, Karine; O'Shea, Siobhan; Pérez-Rivilla, Alfredo; Ramble, John; Trimoulet, Pascale; Vila, Jordi; Whittaker, Duncan; Artus, Alain; Rhodes, Daniel


    Viral load monitoring is essential for patients under treatment for HIV. Beckman Coulter has developed the VERIS HIV-1 Assay for use on the novel, automated DxN VERIS Molecular Diagnostics System. ¥ OBJECTIVES: Evaluation of the clinical performance of the new quantitative VERIS HIV-1 Assay at multiple EU laboratories. Method comparison with the VERIS HIV-1 Assay was performed with 415 specimens at 5 sites tested with COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1 Test, v2.0, 169 specimens at 3 sites tested with RealTime HIV-1 Assay, and 202 specimens from 2 sites tested with VERSANT HIV-1 Assay. Patient monitoring sample results from 4 sites were also compared. Bland-Altman analysis showed the average bias between VERIS HIV-1 Assay and COBAS HIV-1 Test, RealTime HIV-1 Assay, and VERSANT HIV-1 Assay to be 0.28, 0.39, and 0.61 log 10 cp/mL, respectively. Bias at low end levels below 1000cp/mL showed predicted bias to be <0.3 log 10 cp/mL for VERIS HIV-1 Assay versus COBAS HIV-1 Test and RealTime HIV-1 Assay, and <0.5 log 10 cp/mL versus VERSANT HIV-1 Assay. Analysis on 174 specimens tested with the 0.175mL volume VERIS HIV-1 Assay and COBAS HIV-1 Test showed average bias of 0.39 log 10 cp/mL. Patient monitoring results using VERIS HIV-1 Assay demonstrated similar viral load trends over time to all comparators. The VERIS HIV-1 Assay for use on the DxN VERIS System demonstrated comparable clinical performance to COBAS ® HIV-1 Test, RealTime HIV-1 Assay, and VERSANT HIV-1 Assay. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. Evaluation of the highly sensitive Roche thyroglobulin II assay and establishment of a reference limit for thyroglobulin-negative patient samples. (United States)

    Rotteveel-de Groot, Dorien M; Ross, H Alec; Janssen, Marcel J R; Netea-Maier, Romana T; Oosting, Janine D; Sweep, Fred C G J; van Herwaarden, Antonius E


    Thyroglobulin (Tg) measurements are used to monitor for residual thyroid tissue in patients with differentiated thyroid cancer (DTC) after thyroidectomy and radioiodine ablative therapy. In recent years highly sensitive Tg assays have been developed. In this study the analytical performance of the new Roche Elecsys Tg II assay was evaluated and compared with the well documented Access2 Tg assay (Beckman-Coulter). Analytical performance was examined using various Clinical and Laboratory Standards Institute (CLSI) evaluation protocols. Tg negative patient sera were used to establish an upper reference limit (URL) for the Elecsys Tg II assay. Non-linearity, drift and carry-over according to CLSI EP10 and EP6 in a measuring range of 0.04-500 ng/mL were non-significant. Total precision according to CLSI EP5 was 10% at a Tg concentration of 0.08 ng/mL. A patient serum comparison performed according to a modified CLSI EP9 protocol showed a significant difference of a factor of approximately 1.4, despite using an identical CRM calibrator. The Elecsys Tg II assay measured Tg with a two-fold higher sensitivity than the Access2 assay. Finally, using human sera without Tg, an URL of 0.05 ng/mL was determined. In our hands the highly sensitive Elecsys Tg II assay shows a good analytical performance and a higher sensitivity compared to the Access2 Tg assay. An URL of 0.05 ng/mL for the Elecsys Tg II assay was determined which may improve the clinical utility of the assay for the detection of residual DTC or disease recurrence.

  12. Are fixed grain size ratios useful proxies for loess sedimentation dynamics? Experiences from Remizovka, Kazakhstan (United States)

    Schulte, Philipp; Sprafke, Tobias; Rodrigues, Leonor; Fitzsimmons, Kathryn E.


    Loess-paleosol sequences (LPS) are sensitive terrestrial archives of past aeolian dynamics and paleoclimatic changes within the Quaternary. Grain size (GS) analysis is commonly used to interpret aeolian dynamics and climate influences on LPS, based on granulometric parameters such as specific GS classes, ratios of GS classes and statistical manipulation of GS data. However, the GS distribution of a loess sample is not solely a function of aeolian dynamics; rather complex polygenetic depositional and post-depositional processes must be taken into account. This study assesses the reliability of fixed GS ratios as proxies for past sedimentation dynamics using the case study of Remizovka in southeast Kazakhstan. Continuous sampling of the upper 8 m of the profile, which shows extremely weak pedogenic alteration and is therefore dominated by primary aeolian activity, indicates that fixed GS ratios do not adequately serve as proxies for loess sedimentation dynamics. We find through the calculation of single value parameters, that "true" variations within sensitive GS classes are masked by relative changes of the more frequent classes. Heatmap signatures provide the visualization of GS variability within LPS without significant data loss within the measured classes of a sample, or across all measured samples. We also examine the effect of two different commonly used laser diffraction devices on GS ratio calculation by duplicate measurements, the Beckman Coulter (LS13320) and a Malvern Mastersizer Hydro (MM2000), as well as the applicability and significance of the so-called "twin peak ratio" previously developed on samples from the same section. The LS13320 provides higher resolution results than the MM2000, nevertheless the GS ratios related to variations in the silt-sized fraction were comparable. However, we could not detect a twin peak within the coarse silt as detected in the original study using the same device. Our GS measurements differ from previous works at

  13. Comparison of Amicus and COBE Spectra for allogenic peripheral blood stem cell harvest: Study from tertiary care centre in India. (United States)

    Setia, Rasika Dhawan; Arora, Satyam; Handoo, Anil; Dadu, Tina; Choudhary, Dharma; Sharma, Sajeev Kumar; Kharya, Gaurav; Khandelwal, Vipin; Sachdeva, Prerna; Doval, Divya; Bakliwal, Anamika; Kapoor, Meenu; Bajaj, Shalu; Bachchas, Virendra; Singh, Praveen


    Most common source of stem cell graft for both autologous and allogenic haematopoietic transplants are peripheral blood haematopoietic progenitor stem cells. Adequate collection of the CD34+ cells and safety of the allogenic donor during the leukapheresis are of prime importance to an apheresis physician. Our retrospective analysis is a comparison between of two platforms namely, COBE Spectra and Amicus, for CD34+ mononuclear cell collection. The study included the data of GSCF (Granulocyte-Colony-Stimulating Factor) mobilized allogenic PBSC collections at our centre from January 2015 to June 2016. The apheresis platforms used were COBE Spectra and Amicus. Blood cell counts were done using LH750 Beckman Coulter (Florida, Miami, USA). CD45+ & CD34+ cell counts were done using BD FACS Canto-II Flow-Cytometer by ISHAGE guidelines. A total of 170 PBSC (100 COBE Spectra & 70 Amicus) harvests were done on 143 donors, of which 116 completed the collection in a single session and 27 required a second session. Demographic details and pre harvest peripheral blood counts for both the groups did not show any statistical differences. Amicus processed higher blood volume with higher ACD exposure and procedure time compared to COBE Spectra. Higher platelets loss was with COBE Spectra harvests with higher product volumes collection. Collection efficiency (CE2), collection ratio, CD34+ cells dose was similar on both the platforms. RBC contamination, absolute lymphocyte and monocytes counts were significantly higher with Amicus harvest product compared with COBE Spectra. A total of 14 (8.2%; citrate toxicity) adverse reactions were reported out of 170 allogenic PBSC collections. Our study suggests that both Amicus and COBE Spectra platforms offer comparable results for allogenic PBSC collections. Amicus offers a concentrated PBSC product with lesser volume and platelets loss but higher RBC contamination. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Efficient clinical-scale enrichment of lymphocytes for use in adoptive immunotherapy using a modified counterflow centrifugal elutriation program. (United States)

    Powell, Daniel J; Brennan, Andrea L; Zheng, Zhaohui; Huynh, Hong; Cotte, Julio; Levine, Bruce L


    Clinical-scale lymphocyte enrichment from a leukapheresis product has been performed most routinely using costly magnetic bead separation systems that deplete monocytes, but this procedure may leave behind residual beads or antibodies in the enriched cell product. Counterflow centrifugal elutriation has been demonstrated previously to enrich monocytes efficiently for generation of dendritic cells. This study describes a modified elutriation procedure for efficient bead-free economical enrichment of lymphocytes from leukapheresis products from healthy donors and study subjects with human immunodeficiency virus (HIV) infection or malignancy. Modified program settings and conditions for the CaridianBCT Elutra device were investigated to optimize lymphocyte enrichment and recovery. Lymphocyte enrichment was measured using a novel approach utilizing cell sizing analysis on a Beckman Coulter Multisizer and confirmed by flow cytometry phenotypic analysis. Efficient enrichment and recovery of lymphocytes from leukapheresis cell products was achieved using modified elutriation settings for flow rate and fraction volume. Elutriation allowed for enrichment of larger numbers of lymphocytes compared with depletion of monocytes by bead adherence, with a trend toward increased lymphocyte purity and yield via elutriation, resulting in a substantial reduction in the cost of enrichment per cell. Importantly, significant lymphocyte enrichment could be accomplished using leukapheresis samples from healthy donors (n=12) or from study subjects with HIV infection (n=15) or malignancy (n=12). Clinical-scale closed-system elutriation can be performed efficiently for the selective enrichment of lymphocytes for immunotherapy protocols. This represents an improvement in cost, yield and purity over current methods that require the addition of monocyte-depleting beads.

  15. The Effects of Short-Term Intensive Exercise on Levels of Liver Enzymes and Serum Lipids in Kick Boxing Athletes

    Directory of Open Access Journals (Sweden)

    Ömer Kaynar


    Full Text Available Objective: In this study, it was aimed to evaluate the ef­fects of short-term intensive exercise on liver enzymes and serum lipid levels with kick boxing athletes. Methods: 23 voluntary athletes who were between the ages of 15-46 and who engaged in kick–boxing have tak­en place this study. Athletes were made to do 45 minutes of warming-up, breathing, and stretching and 50 minutes of technical and tactical practices and then they were made to do a training match, which is equal to a 2 min­utes 3 circuits (1 minute rest kick-box match. In venous blood samples which were taken from athletes before and after training, aspartate aminotransferase (AST, alanine aminotransferase (ALT, alkaline phosphatase (ALP and gamma glutamine transpeptidase (GGT, enzyme activity and total cholesterol, high-density lipoprotein-cholesterol (HDL-C, low density lipoprotein-cholesterol (LDL-C and triglycerides serum levels were analyzed via spectropho­tometric method in Beckman Coulter AU 5800 auto ana­lyzer. Body composition measurements of athletes were made with Tanita TBF 300 brand device, which works with bio-impedance analysis (BIA system. Results: As a result of our study, statistically increases in serum ALT, AST, ALP and GGT enzyme activities and in serum total cholesterol, HDL-C and LDL-C levels were detected following short-term intensive exercise, but no significant difference was observed in TG levels after in­tensive exercise. Conclusion: The blows to the abdomen during kickbox­ing sports competitions result in increased liver enzymes and increased serum lipids may occur to meet energy de­mand of the body during exercise.

  16. Evaluation of a lateral flow-based technology card for blood typing using a simplified protocol in a model of extreme blood sampling conditions. (United States)

    Clavier, Benoît; Pouget, Thomas; Sailliol, Anne


    Life-threatening situations requiring blood transfusion under extreme conditions or in remote and austere locations, such as the battlefield or in traffic accidents, would benefit from reliable blood typing practices that are easily understood by a nonscientist or nonlaboratory technician and provide quick results. A simplified protocol was developed for the lateral flow-based device MDmulticard ABO-D-Rh subgroups-K. Its performance was compared to a reference method (PK7300, Beckman Coulter) in native blood samples from donors. The method was tested on blood samples stressed in vitro as a model of hemorrhage cases (through hemodilution using physiologic serum) and dehydration (through hemoconcentration by removing an aliquot of plasma after centrifugation), respectively. A total of 146 tests were performed on 52 samples; 126 in the hemodilution group (42 for each native, diluted 1/2, and diluted 1/4 samples) and 20 in the hemoconcentration group (10 for each native and 10% concentrated samples). Hematocrit in the tested samples ranged from 9.8% to 57.6% while hemoglobin levels ranged from 3.2 to 20.1 g/dL. The phenotype profile detected with the MDmulticard using the simplified protocol resulted in 22 A, seven B, 20 O, and three AB, of which nine were D- and five were Kell positive. No discrepancies were found with respect to the results obtained with the reference method. The simplified protocol for MDmulticard use could be considered a reliable method for blood typing in extreme environment or emergency situations, worsened by red blood cell dilution or concentration. © 2017 AABB.

  17. Key performance indicators score (KPIs-score) based on clinical and laboratorial parameters can establish benchmarks for internal quality control in an ART program. (United States)

    Franco, José G; Petersen, Claudia G; Mauri, Ana L; Vagnini, Laura D; Renzi, Adriana; Petersen, Bruna; Mattila, M C; Comar, Vanessa A; Ricci, Juliana; Dieamant, Felipe; Oliveira, João Batista A; Baruffi, Ricardo L R


    KPIs have been employed for internal quality control (IQC) in ART. However, clinical KPIs (C-KPIs) such as age, AMH and number of oocytes collected are never added to laboratory KPIs (L-KPIs), such as fertilization rate and morphological quality of the embryos for analysis, even though the final endpoint is the evaluation of clinical pregnancy rates. This paper analyzed if a KPIs-score strategy with clinical and laboratorial parameters could be used to establish benchmarks for IQC in ART cycles. In this prospective cohort study, 280 patients (36.4±4.3years) underwent ART. The total KPIs-score was obtained by the analysis of age, AMH (AMH Gen II ELISA/pre-mixing modified, Beckman Coulter Inc.), number of metaphase-II oocytes, fertilization rates and morphological quality of the embryonic lot. The total KPIs-score (C-KPIs+L-KPIs) was correlated with the presence or absence of clinical pregnancy. The relationship between the C-KPIs and L-KPIs scores was analyzed to establish quality standards, to increase the performance of clinical and laboratorial processes in ART. The logistic regression model (LRM), with respect to pregnancy and total KPIs-score (280 patients/102 clinical pregnancies), yielded an odds ratio of 1.24 (95%CI = 1.16-1.32). There was also a significant difference (pclinical pregnancies (total KPIs-score=20.4±3.7) and the group without clinical pregnancies (total KPIs-score=15.9±5). Clinical pregnancy probabilities (CPP) can be obtained using the LRM (prediction key) with the total KPIs-score as a predictor variable. The mean C-KPIs and L-KPIs scores obtained in the pregnancy group were 11.9±2.9 and 8.5±1.7, respectively. Routinely, in all cases where the C-KPIs score was ≥9, after the procedure, the L-KPIs score obtained was ≤6, a revision of the laboratory procedure was performed to assess quality standards. This total KPIs-score could set up benchmarks for clinical pregnancy. Moreover, IQC can use C-KPIs and L-KPIs scores to detect problems

  18. Point-of-Care Versus Central Laboratory Measurements of Hemoglobin, Hematocrit, Glucose, Bicarbonate and Electrolytes: A Prospective Observational Study in Critically Ill Patients.

    Directory of Open Access Journals (Sweden)

    Jérôme Allardet-Servent

    Full Text Available Rapid detection of abnormal biological values using point-of-care (POC testing allows clinicians to promptly initiate therapy; however, there are concerns regarding the reliability of POC measurements. We investigated the agreement between the latest generation blood gas analyzer and central laboratory measurements of electrolytes, bicarbonate, hemoglobin, hematocrit, and glucose.314 paired samples were collected prospectively from 51 critically ill patients. All samples were drawn simultaneously in the morning from an arterial line. BD Vacutainer tubes were analyzed in the central laboratory using Beckman Coulter analyzers (AU 5800 and DxH 800. BD Preset 3 ml heparinized-syringes were analyzed immediately in the ICU using the POC Siemens RAPIDPoint 500 blood gas system. We used CLIA proficiency testing criteria to define acceptable analytical performance and interchangeability.Biases, limits of agreement (±1.96 SD and coefficients of correlation were respectively: 1.3 (-2.2 to 4.8 mmol/L, r = 0.936 for sodium; 0.2 (-0.2 to 0.6 mmol/L, r = 0.944 for potassium; -0.9 (-3.7 to 2 mmol/L, r = 0.967 for chloride; 0.8 (-1.9 to 3.4 mmol/L, r = 0.968 for bicarbonate; -11 (-30 to 9 mg/dL, r = 0.972 for glucose; -0.8 (-1.4 to -0.2 g/dL, r = 0.985 for hemoglobin; and -1.1 (-2.9 to 0.7%, r = 0.981 for hematocrit. All differences were below CLIA cut-off values, except for hemoglobin.Compared to central Laboratory analyzers, the POC Siemens RAPIDPoint 500 blood gas system satisfied the CLIA criteria of interchangeability for all tested parameters, except for hemoglobin. These results are warranted for our own procedures and devices. Bearing these restrictions, we recommend clinicians to initiate an appropriate therapy based on POC testing without awaiting a control measurement.

  19. [Use of [-2] pro PSA and phi index for early detection of prostate cancer: a prospective of 452 patients]. (United States)

    Houlgatte, A; Vincendeau, S; Desfemmes, F; Ramirez, J; Benoist, N; Bensalah, K; Durand, X


    Early detection of prostate cancer (Pca) is a real challenge to reduce morbidity and mortality while avoiding over-diagnosis and over-treatment. The prostate specific antigen (PSA) is characterized by its imperfections justifying the evaluation of new serum or urinary specific markers allowing a better selection of patients at risk of developing aggressive Pca. To compare the value of -2pro PSA and phi index to total and free PSA. Serum sampled from 452 patients from two university centers were used to determine levels of PSA before performing biopsies. The patients were included in this study based on the PSA serum concentration between 1.6 ng/mL and 8 ng/mL according to the WHO international standard. All biopsies were performed according to a standardized protocol consisting of 12 cores or more. Sera were analyzed centrally in one of the two institutions with on a single analyzer. Sera from 243 prostate cancer and 208 negative biopsies patients have been taken into account. Sera were analyzed blinded for total PSA, free PSA and [-2] proPSA using Access(®) immunoassay method from Beckman Coulter. The Prostate Health Index (phi) was calculated using the formula phi=([-2] proPSA/fPSA)×sqrt (PSA). The median value of the phi index is significantly (P>0.0001) higher for patients with cancer (phi=65.8) compared to patients with negative biopsies (phi=40.6). At a given sensitivity, the phi index significantly increases the specificity of detection of prostate cancer compared to other markers. The phi index currently appears as the best predictor of prostate cancer for patients with a total PSA between 1.6 and 8 ng/mL according to the WHO standard. The improvement in specificity of the phi index over tPSA could reduce significantly the numbers of unnecessary biopsies. Whether this new biomarker could be an indicator of aggressive prostate cancer remains to be confirmed. Copyright © 2011 Elsevier Masson SAS. All rights reserved.

  20. Simultaneous analysis of free and humic acid complexed europium and gadolinium species by CE-ICP-MS

    International Nuclear Information System (INIS)

    Kautenburger, R.; Nowotka, K.; Beck, H.P.


    Full text of publication follows: For the long-term safety assessment of waste repositories, detailed information about geo-chemical behaviour of radioactive and toxic metal ions under environmental conditions (geological matrix and aquifer systems) is necessary. It includes knowledge about the mechanism of relevant geochemical reactions, as well as thermodynamic and kinetic data. Several previous studies have shown that humic acid can play an important role in the immobilisation or mobilization of metal ions due to complexation and colloid formation. In this project we investigate the complexation behaviour of humic acid (purified Aldrich humic acid) and its influence on the migration of the lanthanides europium and gadolinium (homologues of the actinides americium and curium) in the the ternary system consisting of these heavy metals, humic acid and kaolinite (KGa-1b) as geological model system under conditions close to nature. Capillary electrophoresis (CE, Beckman Coulter P/ACE MDQ), with its excellent separation performance, was coupled to Inductively Coupled Plasma Mass Spectrometry (ICP-MS, VG Elemental Plasma Quad 3) to obtain a high sensitivity for the determination of the rare earth elements europium (Eu 3+ ) and gadolinium (Gd 3+ ) and their complexes with humic acid. Additionally, the used humic acid was halogenated with iodine as ICP-MS marker. A fused-silica capillary was flexibly fitted into a MicroMist 50 μl nebulizer with a Cinnabar cyclonic spray chamber. The chamber was chilled to a temperature of 4 deg. C for best sensitivity. 200 ppb of caesium were added to the CE separation buffer to observe the capillary flow. A make-up fluid including 4 ppb Ho as an internal standard was combined with the flow from the capillary within the interface to obtain a fluid throughput high enough to maintain a continuous nebulization. Very low detection limits were achieved, 100 ppt for 153 Eu and 125 ppt for 158 Gd. With this optimized CE-ICP-MS coupling

  1. Measurement of particle size distribution of soil and selected aggregate sizes using the hydrometer method and laser diffractometry (United States)

    Guzmán, G.; Gómez, J. A.; Giráldez, J. V.


    Soil particle size distribution has been traditionally determined by the hydrometer or the sieve-pipette methods, both of them time consuming and requiring a relatively large soil sample. This might be a limitation in situations, such as for instance analysis of suspended sediment, when the sample is small. A possible alternative to these methods are the optical techniques such as laser diffractometry. However the literature indicates that the use of this technique as an alternative to traditional methods is still limited, because the difficulty in replicating the results obtained with the standard methods. In this study we present the percentages of soil grain size determined using laser diffractometry within ranges set between 0.04 - 2000 μm. A Beckman-Coulter ® LS-230 with a 750 nm laser beam and software version 3.2 in five soils, representative of southern Spain: Alameda, Benacazón, Conchuela, Lanjarón and Pedrera. In three of the studied soils (Alameda, Benacazón and Conchuela) the particle size distribution of each aggregate size class was also determined. Aggregate size classes were obtained by dry sieve analysis using a Retsch AS 200 basic ®. Two hundred grams of air dried soil were sieved during 150 s, at amplitude 2 mm, getting nine different sizes between 2000 μm and 10 μm. Analyses were performed by triplicate. The soil sample preparation was also adapted to our conditions. A small amount each soil sample (less than 1 g) was transferred to the fluid module full of running water and disaggregated by ultrasonication at energy level 4 and 80 ml of sodium hexametaphosphate solution during 580 seconds. Two replicates of each sample were performed. Each measurement was made for a 90 second reading at a pump speed of 62. After the laser diffractometry analysis, each soil and its aggregate classes were processed calibrating its own optical model fitting the optical parameters that mainly depends on the color and the shape of the analyzed particle. As a

  2. Point-of-Care Hemoglobin/Hematocrit Testing: Comparison of Methodology and Technology. (United States)

    Maslow, Andrew; Bert, Arthur; Singh, Arun; Sweeney, Joseph


    Point-of-care (POC) testing allows rapid assessment of hemoglobin (Hgb) and hematocrit (Hct) values. This study compared 3 POC testing devices--the Radical-7 pulse oximeter (Radical-7, Neuchȃtel, Switzerland), the i-STAT (Abbott Point of Care, Princeton, NJ), and the GEM 4000 (Instrumentation Laboratory, Bedford, MA)--to the hospital reference device, the UniCel DxH 800 (Beckman Coulter, Brea, CA) in cardiac surgery patients. Prospective study. Tertiary care cardiovascular center. Twenty-four consecutive elective adult cardiac surgery patients. Hgb and Hct values were measured using 3 POC devices (the Radical-7, i-STAT, and GEM 4000) and a reference laboratory device (UniCel DxH 800). Data were collected simultaneously before surgery, after heparin administration, after heparin reversal with protamine, and after sternal closure. Data were analyzed using bias analyses. POC testing data were compared with that of the reference laboratory device. Hgb levels ranged from 6.8 to 15.1 g/dL, and Hct levels ranged from 20.1% to 43.8%. The overall mean bias was lowest with the i-STAT (Hct, 0.22%; Hgb 0.05 g/dL) compared with the GEM 4000 (Hct, 2.15%; Hgb, 0.63 g/dL) and the Radical-7 (Hgb 1.16 g/dL). The range of data for the i-STAT and Radical-7 was larger than that with the GEM 4000, and the pattern or slopes changed significantly with the i-STAT and Radical-7, whereas that of the GEM 4000 remained relatively stable. The GEM 4000 demonstrated a consistent overestimation of laboratory data, which tended to improve after bypass and at lower Hct/Hgb levels. The i-STAT bias changed from overestimation to underestimation, the latter in the post-cardiopulmonary bypass period and at lower Hct/Hgb levels. By contrast, the Radical-7 biases increased during the surgical procedure and in the lower ranges of Hgb. Important clinical differences and limitations were found among the 3 POC testing devices that should caution clinicians from relying on these data as sole determinants of

  3. Standardization of 8-color flow cytometry across different flow cytometer instruments: A feasibility study in clinical laboratories in Switzerland. (United States)

    Glier, Hana; Heijnen, Ingmar; Hauwel, Mathieu; Dirks, Jan; Quarroz, Stéphane; Lehmann, Thomas; Rovo, Alicia; Arn, Kornelius; Matthes, Thomas; Hogan, Cassandra; Keller, Peter; Dudkiewicz, Ewa; Stüssi, Georg; Fernandez, Paula


    The EuroFlow Consortium developed a fully standardized flow cytometric approach from instrument settings, through antibody panel, reagents and sample preparation protocols, to data acquisition and analysis. The Swiss Cytometry Society (SCS) promoted a study to evaluate the feasibility of using such standardized measurements of 8-color data across two different flow cytometry platforms - Becton Dickinson (BD) FACSCanto II and Beckman Coulter (BC) Navios, aiming at increasing reproducibility and inter-laboratory comparability of immunophenotypic data in clinical laboratories in Switzerland. The study was performed in two phases, i.e. a learning phase (round 1) and an analytical phase (rounds 2 and 3) consisting of a total of three rounds. Overall, 10 laboratories using BD FACSCanto II (n=6) or BC Navios (n=4) flow cytometers participated. Each laboratory measured peripheral blood samples from healthy donors stained with a uniform antibody panel of reagents - EuroFlow Lymphoid Screening Tube (LST) - applying the EuroFlow standardized protocols for instrument setup and sample preparation ( All data files were analyzed centrally and median fluorescence intensity (MedFI) values for individual markers on defined lymphocyte subsets were recorded; variability from reference MedFI values was assessed using performance scores. Data troubleshooting and discussion of the results with the participants followed after each round at SCS meetings. The results of the learning phase demonstrated that standardized instrument setup and data acquisition are feasible in routine clinical laboratories without previous experience with EuroFlow. During the analytical phase, highly comparable data were obtained at the different laboratories using either BD FACSCanto II or BC Navios. The coefficient of variation of MedFI for 7 of 11 markers performed repeatedly below 30%. In the last study round, 89% of participants scored over 90% MedFI values within the acceptance criteria

  4. Clinical chemistry measurements with commercially available test slides on a smartphone platform: Colorimetric determination of glucose and urea. (United States)

    Wu, Yuanyuan; Boonloed, Anukul; Sleszynski, Neal; Koesdjojo, Myra; Armstrong, Chadd; Bracha, Shay; Remcho, Vincent T


    Rapidly increasing healthcare costs in economically advantaged countries are currently unsustainable, while in many developing nations, even 50-year-old technologies are too expensive to implement. New and unconventional technologies are being explored as solutions to this problem. In this study, we examined the use of a smartphone as the detection platform for 2 well-developed, relatively inexpensive, commercially available clinical chemistry assays as a model for rapid and inexpensive clinical diagnostic testing. An Apple iPhone 4 camera phone equipped with a color analysis application (ColorAssist) was combined with Vitros® glucose and urea colorimetric assays. Color images of assay slides at various concentrations of glucose or urea were collected with the iPhone 4 and quantitated in three different spectral ranges (red/green/blue or RGB) using the ColorAssist app. When the diffuse reflectance data was converted into absorbance, it was possible to quantitate glucose or blood urea nitrogen (BUN) over their clinically important concentration ranges (30-515mg/dl for glucose or 2-190mg/dl for BUN), with good linearity (R(2)=0.9994 or 0.9996, respectively [n=5]). Data collected using the iPhone 4 and canine serum samples were in agreement with results from the instrumental "gold standard" (Beckman Coulter AU480 Chemistry System) (R(2)=0.9966 and slope=1.0001 for glucose; R(2)=0.9958 and slope=0.9454 for BUN). Glucose determinations of serum samples made using this smartphone method were as accurate as or more accurate than a commercial colorimetric dry slide analyzer (Heska® Element DC Chemistry Analyzer, Loveland, CO) and 2 glucometers: ReliOn® Ultima (Abbott Diabetes Care Inc) and Presto® (AgaMatrix Inc.H). BUN determinations made using the smartphone approach were comparable in accuracy to the Heska instrument. This demonstration shows that smartphones have the potential to be used as simple, effective colorimetric detectors for quantitative diagnostic tests

  5. Upregulation of genes related to bone formation by γ-amino butyric acid and γ-oryzanol in germinated brown rice is via the activation of GABAB-receptors and reduction of serum IL-6 in rats. (United States)

    Muhammad, Sani Ismaila; Maznah, Ismail; Mahmud, Rozi; Zuki, Abu Bakar Zakaria; Imam, Mustapha Umar


    Osteoporosis and other bone degenerative diseases are among the most challenging non-communicable diseases to treat. Previous works relate bone loss due to osteoporosis with oxidative stress generated by free radicals and inflammatory cytokines. Alternative therapy to hormone replacement has been an area of interest to researchers for almost three decades due to hormone therapy-associated side effects. In this study, we investigated the effects of gamma-amino butyric acid (GABA), gamma-oryzanol (ORZ), acylated steryl glucosides (ASG), and phenolic extracts from germinated brown rice (GBR) on the expression of genes related to bone metabolism, such as bone morphogenic protein-2 (BMP-2), secreted protein acidic and rich in cysteine (SPARC), runt-related transcription factor 2 (RUNX-2), osteoblast-specific transcription factor osterix (Osx), periostin, osteoblast specific factor (Postn), collagen 1&2 (Col1&2), calcitonin receptor gene (CGRP); body weight measurement and also serum interleukin-6 (IL-6) and osteocalcin, in serum and bone. Rats were treated with GBR, ORZ, GABA, and ASG at (100 and 200 mg/kg); estrogen (0.2 mg/kg), or remifemin (10 and 20 mg/kg), compared to ovariectomized non-treated group as well as non-ovariectomized non-treated (sham) group. Enzyme-linked immunosorbent assay was used to measure the IL-6 and osteocalcin levels at week 2, 4, and 8, while the gene expression in the bone tissue was determined using the Genetic Analysis System (Beckman Coulter Inc., Brea, CA, USA). The results indicate that groups treated with GABA (100 and 200 mg/kg) showed significant upregulation of SPARC, calcitonin receptor, and BMP-2 genes (P < 0.05), while the ORZ-treated group (100 and 200 mg/kg) revealed significant (P < 0.05) upregulation of Osx, Postn, RUNX-2, and Col1&2. Similarly, IL-6 concentration decreased, while osteocalcin levels increased significantly (P < 0.05) in the treated groups as compared to ovariectomized non-treated groups. GABA and ORZ from

  6. Case study: improving efficiency in a large hospital laboratory. (United States)

    Bartel, Marilynn


    Saint Francis Health System (SFHS) consists of three hospitals and one clinic: Saint Francis Hospital (SFH); Broken Arrow Medical Center; Laureate Psychiatric Hospital; and Warren Clinic. SFHS has 670 physicians on staff and serves medical (oncology, orthopedic, neurology, and renal), surgical, cardiac, women and infant, pediatric, transplant, and trauma patients in Tulsa County, Oklahoma, which has a population of 660,000. SFH incorporates 706 staffed beds, including 126 pediatric beds and 119 critical care beds. Each year, the health system averages 38,000 admissions, 70,000 emergency department visits, 25,000 surgeries, and 3,500 births. Saint Francis Laboratory is located within the main hospital facility (SFH) and functions as a core lab for the health system. The lab also coordinates lab services with Saint Francis Heart Hospital, a physician-system joint venture. The Optimal Equipment Configuration (OEC) Project was designed by the Clinical Laboratory Services division of Premier, a group purchasing organization, with the goal of determining whether laboratories could improve efficiency and decrease unit cost by using a single-source vendor. Participants included seven business partners (Abbott, Bayer, Beckman/Coulter, Dade/Behring, J&J/ Ortho, Olympus, and Roche) and 21 laboratory sites (a small, mid-sized, and large site for each vendor). SFH laboratory staff embraced Premier's concept and viewed the OEC project as an opportunity to "energize" laboratory operations. SFH partnered with Abbott, their primary equipment vendor, for the project. Using resources and tools made available through the project, the laboratory was re-engineered to simplify workflow, increase productivity, and decrease costs by adding automation and changing to centralized specimen processing. Abbott and SFH shared a common vision for the project and enhanced their partnership through increased communication and problem solving. Abbott's area representatives provided for third

  7. Point-of-Care Versus Central Laboratory Measurements of Hemoglobin, Hematocrit, Glucose, Bicarbonate and Electrolytes: A Prospective Observational Study in Critically Ill Patients. (United States)

    Allardet-Servent, Jérôme; Lebsir, Melissa; Dubroca, Christian; Fabrigoule, Martine; Jordana, Sylvie; Signouret, Thomas; Castanier, Matthias; Thomas, Guillemette; Soundaravelou, Rettinavelou; Lepidi, Anne; Delapierre, Laurence; Penaranda, Guillaume; Halfon, Philippe; Seghboyan, Jean-Marie


    Rapid detection of abnormal biological values using point-of-care (POC) testing allows clinicians to promptly initiate therapy; however, there are concerns regarding the reliability of POC measurements. We investigated the agreement between the latest generation blood gas analyzer and central laboratory measurements of electrolytes, bicarbonate, hemoglobin, hematocrit, and glucose. 314 paired samples were collected prospectively from 51 critically ill patients. All samples were drawn simultaneously in the morning from an arterial line. BD Vacutainer tubes were analyzed in the central laboratory using Beckman Coulter analyzers (AU 5800 and DxH 800). BD Preset 3 ml heparinized-syringes were analyzed immediately in the ICU using the POC Siemens RAPIDPoint 500 blood gas system. We used CLIA proficiency testing criteria to define acceptable analytical performance and interchangeability. Biases, limits of agreement (±1.96 SD) and coefficients of correlation were respectively: 1.3 (-2.2 to 4.8 mmol/L, r = 0.936) for sodium; 0.2 (-0.2 to 0.6 mmol/L, r = 0.944) for potassium; -0.9 (-3.7 to 2 mmol/L, r = 0.967) for chloride; 0.8 (-1.9 to 3.4 mmol/L, r = 0.968) for bicarbonate; -11 (-30 to 9 mg/dL, r = 0.972) for glucose; -0.8 (-1.4 to -0.2 g/dL, r = 0.985) for hemoglobin; and -1.1 (-2.9 to 0.7%, r = 0.981) for hematocrit. All differences were below CLIA cut-off values, except for hemoglobin. Compared to central Laboratory analyzers, the POC Siemens RAPIDPoint 500 blood gas system satisfied the CLIA criteria of interchangeability for all tested parameters, except for hemoglobin. These results are warranted for our own procedures and devices. Bearing these restrictions, we recommend clinicians to initiate an appropriate therapy based on POC testing without awaiting a control measurement.

  8. Study of urinary leukotriene E4 in atopic dermatitis: relation to ...

    African Journals Online (AJOL)


    I-Complete blood count using Coulter cell counter. T660 (Coulter corporation, Florida, USA) with examination ... The results displayed are corrected values. IV- Quantitative determination of serum IgE concentrations: 2 ml of venous blood were collected in a tube, left to clot and centrifuged at. 1500 rpm to separate the serum.

  9. Anaemia in pregnant women in eastern Caprivi, Namibia

    African Journals Online (AJOL)

    Iron deficiency associated with depleted stores and ... concentrations and iron deficiency can be linked to maternal and fetal ... saturation with Beckman Synchron GX systems. Because .... mineral supplementation seems to offer little protection.

  10. International Journal of Health Research

    African Journals Online (AJOL)


    for each clinical chemistry laboratory to establish its own ranges. Keywords: ... In clinical management of patients, ... physician have good reference information. ... Control (QC) sample (Multi Sera) using Beckman Synchron System CX5.

  11. 75 FR 38504 - Army Science Board Plenary Meeting (United States)


    ... Soldier Resilience and Performance Sustainment. Purposed Agenda: Wednesday 21 July: 0800-0930 Study... Meeting: July 21, 2010. Time(s) of Meeting: 0800-1330. Location: Beckman Center, 100 Academy, Irvine, CA...

  12. Findings in indigenous West African men using

    African Journals Online (AJOL)

    K.H. Tijani

    Available online 24 December 2016 ... The technique of PSA analysis was the Access hybritech assay technique using the Beckman's ... It is the only public hospital ..... [11]

  13. Comparison of a new rapid plasma reagin card test with the standard rapid plasma reagin 18-mm circle card test and the venereal disease research laboratory slide test for serodiagnosis of syphilis.


    Hambie, E A; Larsen, S A; Perryman, M W; Pettit, D E; Mullally, R L; Whittington, W


    The rapid plasma reagin (RPR) card test manufactured by Beckman Instruments, Inc., was compared, qualitatively and quantitatively, with the Venereal Disease Research Laboratory (VDRL) slide test and the standard RPR 18-mm circle card tests for the serodiagnosis of syphilis. Sera from 638 individuals were used in this study. Two pilot lots and two production lots of antigen were submitted by Beckman Instruments, Inc., for evaluation. Qualitative agreement among the three RPR card tests was 98....

  14. Use of blood biomarkers to screen for obstructive sleep apnea

    Directory of Open Access Journals (Sweden)

    Fleming WE


    Full Text Available Wesley Elon Fleming,1 Jon-Erik C Holty,2 Richard K Bogan,3 Dennis Hwang,4 Aliya S Ferouz-Colborn,4 Rohit Budhiraja,5 Susan Redline,5 Edith Mensah-Osman,6 Nadir Ishag Osman,6 Qing Li,7 Armaghan Azad,1 Susann Podolak,1 Michael K Samoszuk,8 Amabelle B Cruz,8 Yang Bai,8 Jiuliu Lu,8 John S Riley,8 Paula C Southwick8 1Sleep Center Orange County, Irvine, CA, USA; 2Stanford Medical School, VA Palo Alto Health Care System, Pulmonary, Critical Care and Sleep Medicine Section, Palo Alto, CA, USA; 3SleepMed Inc., Bogan Sleep Consultants, LLC, Columbia, SC, USA; 4Sleep Medicine Department, Southern California Permanente Medical Group, Kaiser Permanente, Fontana Medical Center, Fontana, CA, USA; 5Division of Sleep Medicine, Harvard Medical School, Brigham and Women’s Hospital, Boston, MA, USA; 6EENA Comprehensive Neurology and Sleep Center, Boynton Beach, FL, USA; 7South Bend Medical Foundation, New Technology and Test Development, South Bend, IN, USA; 8Clinical Research Department, Beckman Coulter, Inc., Brea, CA, USAPurpose: Obstructive sleep apnea (OSA is a highly prevalent disorder associated with increased risk for cardiovascular disease, diabetes, and other chronic conditions. Unfortunately, up to 90% of individuals with OSA remain without a diagnosis or therapy. We assess the relationship between OSA and blood biomarkers, and test the hypothesis that combinations of markers provide a characteristic OSA signature with diagnostic screening value. This validation study was conducted in an independent cohort in order to replicate findings from a prior feasibility study.Patients and methods: This multicenter prospective study consecutively enrolled adult male subjects with clinically suspected OSA. All subjects underwent overnight sleep studies. An asymptomatic control group was also obtained. Five biomarkers were tested: glycated hemoglobin (HbA1c, C-reactive protein (CRP, uric acid, erythropoietin (EPO, and interleukin-6 (IL-6.Results: The study

  15. The DOE fellows program-a workforce development initiative for the US department of energy

    Energy Technology Data Exchange (ETDEWEB)

    Lagos, Leonel E. [Applied Research Center, Florida International University, 10555 West Flagler St, EC2100, Miami, Florida (United States)


    posters and 7 oral presentations given at WM. The DOE Fellows participation at WM has resulted in three Best Student Poster Awards (WM09, WM10, and WM11) and one Best Professional Poster Award (WM09). DOE Fellows have also presented their research at ANS DD and R and ANS Robotics Topical meetings and this year two Fellows will present at the International Conference on Environmental Remediation and Radioactive Waste Management (ICEM13) in Brussels, Belgium. Moreover, several of our DOE Fellows have already obtained employment with DOE-EM, other federal agencies, DOE contractors, commercial nuclear power companies, and other STEM industry (GE, Boeing, Lockheed Martin, Johnson and Johnson, Beckman-Coulter, and other top companies). This paper will discuss how DOE Fellows program is training and mentoring FIU STEM students in Department of Energy's Office of Environmental Management technical challenges and research. This training and mentoring has resulted in the development of well-trained and polished young scientists and engineers that will become the future workforce in charge of carrying on DOE-EM's environmental cleanup mission. The paper will showcase FIU's DOE Fellows model and highlight some of the applied research the DOE Fellows have conducted at FIU's Applied Research Center and across the DOE Complex by participating in summer internship assignments. (authors)

  16. Elevated levels of beta-human chorionic gonadotropin and human placental lactogen between 11-13 week's gestation and subsequent pregnancy complications in Oman i women

    International Nuclear Information System (INIS)

    Krolikowski, Andrzej; Al-Busaidi, Fikra; Al-Wahaibi, Adil


    The association between abnormal levels of maternal serum b-human chorionic gonadotrophin (b-HCG) and human placental lactogen (HPL) measured in early pregnancy and future poor pregnancy outcome is fairly well established. Little is known on how such prognostic information can be used to avoid future complications of pregnancy and improve their outcome. We undertook a prospective study, which was designed to assess the efficiency of maternal serum biochemical markers b-HCG and HPL for the detection of different pregnancy and labor complications. The blood samples were taken from pregnant patients who attended the antenatal clinic at Sultan Qaboos University Hospital in Muscat, Sultanate of Oman, from December 2001 to October 2002. These patients were selected randomly. A total of 200 Omani women, none diabetic, with singleton pregnancies between 11 and 13 weeks gestation were recruited into the study. Gestational age was calculated from the first day of the last menstrual period, unless ultrasonography showed a discrepancy of more than 14 days. Excluded from the study were pregnancies with fetal anomalies, multiple gestations and insulin dependent maternal diabetes. Biochemical analysis of both maternal serum markers was performed in the clinical biochemistry laboratory using an automated immunometric technique supplied by Beckman Coulter for b-HCG and manual radioimmunoassay utilizing a gamma radiation scintillation counter for HPL. Both assays underwent internal, trilevel quality controls. The selected patients were monitored for the following complications: pregnancy induced hypertension (PIH), gestational diabetes, polyhydramnios, antepartum hemorrhage, intrauterine growth retardation (IUGR) (birth weight <10th per centile for gestation), low Apgar score (7 or less) and emergency cesarean section. 75 patients did not develop any pregnancy complications and delivered vaginally. This was our control group. 85 patients developed one of the above mentioned problems

  17. The percentage of prostate-specific antigen (PSA) isoform [-2]proPSA and the Prostate Health Index improve the diagnostic accuracy for clinically relevant prostate cancer at initial and repeat biopsy compared with total PSA and percentage free PSA in men aged ≤65 years. (United States)

    Boegemann, Martin; Stephan, Carsten; Cammann, Henning; Vincendeau, Sébastien; Houlgatte, Alain; Jung, Klaus; Blanchet, Jean-Sebastien; Semjonow, Axel


    To prospectively test the diagnostic accuracy of the percentage of prostate specific antigen (PSA) isoform [-2]proPSA (%p2PSA) and the Prostate Health Index (PHI), and to determine their role for discrimination between significant and insignificant prostate cancer at initial and repeat prostate biopsy in men aged ≤65 years. The diagnostic performance of %p2PSA and PHI were evaluated in a multicentre study. In all, 769 men aged ≤65 years scheduled for initial or repeat prostate biopsy were recruited in four sites based on a total PSA (t-PSA) level of 1.6-8.0 ng/mL World Health Organization (WHO) calibrated (2-10 ng/mL Hybritech-calibrated). Serum samples were measured for the concentration of t-PSA, free PSA (f-PSA) and p2PSA with Beckman Coulter immunoassays on Access-2 or DxI800 instruments. PHI was calculated as (p2PSA/f-PSA × √t-PSA). Uni- and multivariable logistic regression models and an artificial neural network (ANN) were complemented by decision curve analysis (DCA). In univariate analysis %p2PSA and PHI were the best predictors of prostate cancer detection in all patients (area under the curve [AUC] 0.72 and 0.73, respectively), at initial (AUC 0.67 and 0.69) and repeat biopsy (AUC 0.74 and 0.74). t-PSA and %f-PSA performed less accurately for all patients (AUC 0.54 and 0.62). For detection of significant prostate cancer (based on Prostate Cancer Research International Active Surveillance [PRIAS] criteria) the %p2PSA and PHI equally demonstrated best performance (AUC 0.70 and 0.73) compared with t-PSA and %f-PSA (AUC 0.54 and 0.59). In multivariate analysis PHI we added to a base model of age, prostate volume, digital rectal examination, t-PSA and %f-PSA. PHI was strongest in predicting prostate cancer in all patients, at initial and repeat biopsy and for significant prostate cancer (AUC 0.73, 0.68, 0.78 and 0.72, respectively). In DCA for all patients the ANN showed the broadest threshold probability and best net benefit. PHI as single parameter

  18. The DOE fellows program-a workforce development initiative for the US department of energy

    International Nuclear Information System (INIS)

    Lagos, Leonel E.


    posters and 7 oral presentations given at WM. The DOE Fellows participation at WM has resulted in three Best Student Poster Awards (WM09, WM10, and WM11) and one Best Professional Poster Award (WM09). DOE Fellows have also presented their research at ANS DD and R and ANS Robotics Topical meetings and this year two Fellows will present at the International Conference on Environmental Remediation and Radioactive Waste Management (ICEM13) in Brussels, Belgium. Moreover, several of our DOE Fellows have already obtained employment with DOE-EM, other federal agencies, DOE contractors, commercial nuclear power companies, and other STEM industry (GE, Boeing, Lockheed Martin, Johnson and Johnson, Beckman-Coulter, and other top companies). This paper will discuss how DOE Fellows program is training and mentoring FIU STEM students in Department of Energy's Office of Environmental Management technical challenges and research. This training and mentoring has resulted in the development of well-trained and polished young scientists and engineers that will become the future workforce in charge of carrying on DOE-EM's environmental cleanup mission. The paper will showcase FIU's DOE Fellows model and highlight some of the applied research the DOE Fellows have conducted at FIU's Applied Research Center and across the DOE Complex by participating in summer internship assignments. (authors)

  19. ECOHAB: Kamykowski_D- Florida Process Cruise 11/16-19/1998- Karenia brevis counts, biochemistry and behavior (NODC Accession 0000534) (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Abstract: Water bottle samples were collected from selected depths (variable with station) at 14 stations in the study area. Coulter counts in the 14-28 um size...

  20. ECOHAB: Kamykowski_D- Florida Process Cruise 11/16-19/1998- Karenia brevis counts, biochemistry and behavior (NODC Accession 0000533) (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Abstract: Water bottle samples were collected from selected depths (variable with station) at 44 stations in the study area. Coulter counts in the 14 to 28 um size...

  1. Substitution of Fingertip Blood for Venous Blood in the Measurement of Hematocrit and Hemoglobin Following Exercise (United States)

    Fahey, Thomas D.; And Others


    Results from comparative testing indicate that fingertip blood is a valid indicator of antecubital venous hematocrit (hct) and hemoglobin (hgb), and that hct ratios determined on the Coulter counter are comparable to those found by the microhematocrit method. (MB)

  2. Particle shape accounts for instrumental discrepancy in ice core dust size distributions (United States)

    Folden Simonsen, Marius; Cremonesi, Llorenç; Baccolo, Giovanni; Bosch, Samuel; Delmonte, Barbara; Erhardt, Tobias; Kjær, Helle Astrid; Potenza, Marco; Svensson, Anders; Vallelonga, Paul


    The Klotz Abakus laser sensor and the Coulter counter are both used for measuring the size distribution of insoluble mineral dust particles in ice cores. While the Coulter counter measures particle volume accurately, the equivalent Abakus instrument measurement deviates substantially from the Coulter counter. We show that the difference between the Abakus and the Coulter counter measurements is mainly caused by the irregular shape of dust particles in ice core samples. The irregular shape means that a new calibration routine based on standard spheres is necessary for obtaining fully comparable data. This new calibration routine gives an increased accuracy to Abakus measurements, which may improve future ice core record intercomparisons. We derived an analytical model for extracting the aspect ratio of dust particles from the difference between Abakus and Coulter counter data. For verification, we measured the aspect ratio of the same samples directly using a single-particle extinction and scattering instrument. The results demonstrate that the model is accurate enough to discern between samples of aspect ratio 0.3 and 0.4 using only the comparison of Abakus and Coulter counter data.

  3. Residue management practices and planter attachments for corn production in a conservation agriculture system

    Directory of Open Access Journals (Sweden)

    J. Nejadi


    Full Text Available Seed placement and failure to establish a uniform plant stand are critical problems associated with production of corn (Zea mays following wheat (Triticum aestivum in a conservation agriculture system in Iran. Our objectives were to evaluate the performance of a corn row- crop planter equipped with two planter attachments (smooth/toothed coulters at six wheat residue management systems (three tillage systems and two levels of surface residue at two forward speeds of 5 and 7 km h-1. Residue retained after planting, seeding depth, emergence rate index (ERI and seed spacing indices were determined. The baled residue plots tilled by chisel plow followed by disc harrow (BRCD resulted in minimum residue after planting as compared to other residue treatments. Furthermore, the maximum values of the ERI and uniformity of plant spacing pertained to this treatment. Other results showed that the ERI increased up to 18% for the toothed coulter as compared to the smooth coulter. The toothed coulter also established a deeper seed placement as compared to the smooth coulter. Planting at forward speed of 5 km h-1 resulted in deeper seeding depth as compared to a forward speed of 7 km h-1. However, lower values of miss and precision indices were obtained at forward speed of 7 km h-1, indicating a more uniformity of plant spacing. Results of this study showed that equipping the conventional planter with toothed coulter and planting in soil prepared under the BRCD residue management system can result in a satisfactory conservation crop production system.

  4. Highly Resolved Paleoclimatic Aerosol Records

    DEFF Research Database (Denmark)

    Kettner, Ernesto

    soluble aerosols can be analysed for concentration changes only, insoluble aeolian dust can reveal additional information on its atmospheric residence time via changes in the mean grain sizes. Volumes of particulate matter in ice cores are most reliably determined with Coulter counters, but since...... a Coulter counter performs measurements on discrete samples, it cannot be connected to a CFA system. Attenuation sensors, on the other hand, can be integrated into a CFA set-up, but are known to yield poor dust size records. The dilemma between high quality sizing and high depth resolution was found...

  5. Instrumentation

    Energy Technology Data Exchange (ETDEWEB)



    Developments discussed include a transistorized- power supply for the Beckman DU spectrophotometer, a master clock pulse generator, a modular printing unit, a flash photolysis setup, a glove box assembly for a Perkin-Elmer infrared spectrophotometer, and a Faraday balance for measuring the magnetic susceptibilities of actinide compounds. (WHK)

  6. A Antimi crobia al susc from ceptibi Asper lity ass rgillus sessm ...

    African Journals Online (AJOL)



    Jan 2, 2015 ... Production, isolation and purification of the compounds. The organism was allowed to grow in a number of culture flasks of. 500 ml capacity containing ... with 0.1% vanillin sulfate spray reagent followed by heating. Spectral measurement. Ultra-violet (UV) spectra were recorded on a Beckman double beam.

  7. The SDF-1-CXCR4 Axis Functions Through p38-MAPK Signaling to Drive Breast Cancer Progression and Metastasis (United States)


    2002). Rubin JB, Kung AL, Klein RS, Chan JA, Sun Y, Schmidt K, Kieran MW, Luster AD, Segal RA.A small-molecule antagonist of CXCR4 inhibits... Melanie H. Howell, Michelle Lacey, Betty Y. Shih, Carol Carter-Wientjes, Thomas E. Cleveland, John A. McLachlan, Thomas E. Wiese, Barbara S. Beckman

  8. Nitroguanidine Wastewater Pollution Control Technology: Phase 2 Wastewater Characterization and Analytical Methods Development for Organics (United States)


    a....... .6 Thin Layer Chromatographic (TLC) Analyses... o................. ......a... 7 RESULTS AND DISCUSSIONo.....................o...Beckman 5230 UV/visible spectrophotometer was used for colorimetric determinations of urea and cyanamide. Urea was hydrolyzed by urease and...correlation coefficients were 0.9999. THIN LAYER CHROMATOGRAPHIC (TLC) ANALYSES Cellulose plates were used and were developed in the following systems: 3N

  9. Apoptosis of lymphocytes in SLE: the level, correlation with ...

    African Journals Online (AJOL)

    Lymphocytes were isolated from venous blood by method of gradient centrifugation of all the blood through a Ficoll-pak solution. The quantity apoptotic cells was determined in leukocytes by flow cytometry Epics XL-2 (“Beckman Coulter”, USA). Analysis of lymphocyte subpopulations was carried by using two fluorescent ...

  10. The Impact of Siblings with Disabilities on Their Brothers and Sisters Pursuing a Career in Special Education (United States)

    Marks, Susan Unok; Matson, Amy; Barraza, Lori


    It is well known that growing up with a sibling who has a disability has both positive and negative impacts on the non-disabled sibling (Beckman, 2002; Fisman, Wolf, Ellison, & Freeman, 2000; Gans, 1997; McHugh, 2003). In fact, the concern for ameliorating the potential negative impacts on nondisabled siblings has been a major impetus for the…

  11. Organizational Perceptions of Telecommuting in the Private Sector (United States)

    Galusha, Repps J.


    The Internet has provided more organizations the opportunity to adopt telecommuting as a means to recruit and retain employees, boost productivity, and trim facility costs. This study expands on the work of a previous study by Hoang, Nickerson, Beckman, and Eng, in 2008 which found that private organizations, due to perceptions of organizational…

  12. A one-step separation of human serum high density lipoproteins 2 and 3 by rate-zonal density gradient ultracentrifugation in a swinging bucket rotor

    NARCIS (Netherlands)

    Groot, P.H.E.; Scheek, L.M.; Havekes, L.; Noort, W.L. van; Hooft, F.M. van 't


    A method was developed for the separation of the high density lipoprotein subclasses HDL2 and HDL3 from human serum. Six serum samples are fractionated in a single-step ultracentrifugal procedure using the Beckman (SW-40) swinging bucket rotor. The method is based on a difference in flotation rate

  13. Test plan : Branson TRIP focus groups and personal interviews (United States)


    This is a summary of a workshop that examined the issues raised in connection with the U.S. Department of Transportation's Federal Comprehensive Truck Size and Weight (TS&W) Study. The workshop was held on May 10-11, 2000 at the Beckman Center in Irv...

  14. Monitoring of endocrine disrupting chemicals in surface water

    CSIR Research Space (South Africa)

    Govender, S


    Full Text Available monitored at 25°C in a CARY 110 UV-Vis spectrophotometer. A Beckman spectrophotometric plate reader was used to measure the activity of avidin conjugated peroxidase (Av-P). In the presence of sufficient affinity immobilized Av-P, the ABTS solution...

  15. "Response to Comments": Finding the Narrative in Narrative Research (United States)

    Coulter, Cathy A.


    The author responds to comments by Barone (2009), Clandinin and Murphy (2009), and M. W. Smith (2009) on "The Construction Zone: Literary Elements in Narrative Research" (Coulter & M. L. Smith, 2009). She clarifies issues regarding point of view, authorial surplus, narrative coherence, and the relational qualities of narrative research. She…

  16. Application of particle size distributions to total particulate stack samples to estimate PM2.5 and PM10 emission factors for agricultural sources (United States)

    Particle size distributions (PSD) have long been used to more accurately estimate the PM10 fraction of total particulate matter (PM) stack samples taken from agricultural sources. These PSD analyses were typically conducted using a Coulter Counter with 50 micrometer aperture tube. With recent increa...

  17. limnological studies of the pelagic zone of lake tanganyika at ...

    African Journals Online (AJOL)


    result of advection of deep water into the metalimnion (Plisnier et al. 1999, Coulter. 1963) and also decomposition of dead organic matter. In October, heavy scums of. Anabaena spp. were observed in the study area (Hecky and Kling 1981). Nitrate-nitrogen concentrations in the euphotic zone ranged between 26 µg N l-1 in.

  18. Genetics Home Reference: carbonic anhydrase VA deficiency (United States)

    ... Salvarinova R, Yaplito-Lee J, Santra S, Shyr C, Horvath GA, Eydoux P, Lehman AM, Bernard V, Newlove T, Ukpeh H, Chakrapani A, Preece MA, Ball S, Pitt J, Vallance HD, Coulter-Mackie M, Nguyen H, Zhang LH, Bhavsar AP, Sinclair G, Waheed A, Wasserman WW, Stockler-Ipsiroglu S. Mitochondrial carbonic ...

  19. The challenges of testing metal and metal oxide nanoparticles in algal bioassays: titanium dioxide and gold nanoparticles as case studies

    DEFF Research Database (Denmark)

    Hartmann, Nanna Isabella Bloch; Engelbrekt, Christian; Zhang, Jingdong


    and TiO(2) nanoparticle aggregation/agglomeration increased as a function of concentration. Three biomass surrogate measuring techniques were evaluated (coulter counting, cell counting in haemocytometer, and fluorescence of pigment extracts) and out of these the fluorometric methods was found to be most...

  20. Making Meaning of Student Activism: Student Activist and Administrator Perspectives (United States)

    Harrison, Laura M.; Mather, Peter C.


    College campuses have experienced a recent resurgence of student activism, particularly in response to some of President Donald Trump's executive orders as well as controversial speakers like Ann Coulter and Milo Yiannopoulous. Student activism presents both challenges and opportunities for higher education leaders seeking to engage productively…

  1. Alteration of Bax-to-Bcl-2 ratio modulates the anticancer activity of ...

    African Journals Online (AJOL)



    Oct 20, 2008 ... regulation of cellular response to DNA-damage, cell cycle control and .... counted at different times of incubation using a Coulter counter model Z1 ... in a reverse transcription reaction mixture containing 1x PCR buffer,. 0.5 mM ...

  2. De scheiding van slib en plankton

    NARCIS (Netherlands)

    Budding, M.C.


    It is possible to separate non-living suspended matter and living plankton with the help of a common laboratory centrifuge and a commercial silica-gel called LUDOX. With this method it becomes possible to determine particle size of suspended matter and plankton separately with e.g. a Coulter

  3. Pikantsed pajatused / Aita Kivi

    Index Scriptorium Estoniae

    Kivi, Aita, 1954-


    Sisu : Louis Paul Boon. Menuett; Herbjırg Wassmo. Õnne poeg; Jean Stone. Sünnipäevatüdrukud; Jo︠l Schmidt. Constantinus Suure mälestused; Mari Vallisoo. Ainsuse olevik; Catherine Coulter. Märklaud; Ross Macdonald. Mees mulla all

  4. Aplastic crisis in occult hereditary spherocytosis caused by human parvovirus (HPV B19). (United States)

    Rappaport, E S; Quick, G; Ransom, D; Helbert, B; Frankel, L S


    We have reported a case of aplastic crisis occurring in an 11-year-old black boy with occult hereditary spherocytosis. An etiologic diagnosis of human parvovirus (HPV) B19 infection was confirmed serologically. The Coulter Model S + IV proved useful for both diagnosis and treatment monitoring through serial histograms. The relationship of HPV infection and aplastic crisis is discussed.

  5. Is Dysguesia Going to be a Rare or a Common Side‑effect of ...

    African Journals Online (AJOL)

    amlodipine are peripheral edema (8.3%), fatigue (4.5%), dizziness, palpitations, myalgia, stomachache, headache, dyspepsia, nausea, blood disorders, gynecomastia, .... Coulter DM. Eye pain with nifedipine and disturbance of taste with captopril: A mutually controlled study showing a method of postmarketing surveillance.

  6. 2x Ben Affleck / Laurence Lumiere

    Index Scriptorium Estoniae

    Lumiere, Laurence


    Põnevusfilm "Hollywoodland" : režissöör Allen Coulter : Ameerika Ühendriigid 2006 ja põnevuskomöödia "Äss mättasse" ("Smokin' Aces") : režissöör Joe Carnahan : Suurbritannia - Prantsusmaa - Ameerika Ühendriigid 2007. Mõlemas mängib Ben Affleck

  7. Primary production, nutrients, and size spectra of suspended particles in the southern North Sea

    NARCIS (Netherlands)

    Gieskes, W.W.C.


    The effect of nutrient enrichment from the Rhine on some major characteristics of the phytoplankton ecosystem of Dutch coastal waters was studied with 14C, liquid scintillation and Coulter Counter techniques. The magnitude of primary production in the most eutrophic waters closest to

  8. The Role of VDR in Phosphorylation in Vitamin D-Induced Apoptosis (United States)


    nuclear pellet was resuspended in Buffer A, Cytometer (Coulter Corp., Miami, FL) equipped with an argon laser . sonicated 2 x 10 s, and stored at -80 ’C...rickets type II with alopecia . Proc Natl Acad Sci [45] Mezzetti G, Barbiroli B, Oka T. 1,25-Dihydroxycholecalciferol re- USA 1997;94:9831-5. ceptor

  9. Sorting catalytically active polymersome nanoreactors by flow cytometry

    NARCIS (Netherlands)

    Nallani, M.; Woestenenk, R.; de Hoog, H.P.M.; van Dongen, S.F.M.; Boezeman, J.; Cornelissen, J.J.L.M.; Nolte, R.J.M.; van Hest, J.C.M.


    A strategy that involves a versatile one-step preparation procedure of enzyme filled porous and stable polymeric catalytically active nanoreactors (polymersomes) by flow cytometry was reported. A 1:1 mixture of the polymerase dispersions was analyzed in a Coulter Epics Elite Flow Cytometer, while

  10. Xylem monoterpenes of some hard pines of Western North America: three studies (United States)

    Richard H. Smith


    Monoterpene composition was studied in a number of hard pine species and results were compared with earlier work. (1) Intratree measurements showed strong constancy of composition in both single-stemmed and forked trees of ponderosa, Jeffrey, Coulter, and Jeffrey x ponderosa pines. In grafts of these and other pines, the scion influenced the root stock, but not the...

  11. Department of Clinical Investigation Annual Research Progress Report Fiscal Year 2002 (United States)


    on ice for 20 min. Samples will be centrifuged at 4ºC for 20 min. at 9,500 rpm in a Beckman GS-15R tabletop centrifuge. The aqueous layer will be...positive serology by the presence of either positive H. pylori on histology and a positive rapid urease test. At endoscopy, after aspiration of gastric...laboratory tests and Puality of Life questionnare completed; digital photography will be performed; bi- layer tracing of the wound will be measured

  12. Glovebox enclosed dc plasma source for the determination of metals in plutonium

    International Nuclear Information System (INIS)

    Morris, W.F.


    The direct current plasma source of a Beckman Spectraspan IIIB emission spectrometer was enclosed in a glovebox at Lawrence Livermore National Laboratory in December 1982. Since that time, the system has been used for the routine determination of alloy and impurity metals in plutonium. This paper presents the systematic steps involved in developing the glovebox and gives information regarding performance of the plasma in the glovebox and the effectiveness of containment of plutonium. 8 refs., 9 figs., 3 tabs

  13. Traumatic Floating Clavicle: A Case Report and Literature Review


    Gouse, Mohamad; Jacob, Korula Mani; Poonnoose, Pradeep Mathew


    Bipolar fracture dislocations of the clavicle are rare injuries, usually the result of high-energy direct trauma. Since the original description by Porral in 1831, only a handful of individual case reports and case series by Beckman and Sanders have been reported in the literature. Management of these injuries has remained controversial ranging from nonoperative to aggressive surgery. We report on the case of a young army cadet who had a fracture of the lateral end of the clavicle, with an an...

  14. Airfuge centrifugation procedure for the measurement of ligand binding to membrane-associated and detergent-solubilized plasma membrane receptors

    Energy Technology Data Exchange (ETDEWEB)

    Li, E L.F.; Perdue, J F [Lady Davis Institute, Sir Mortimer B. Davis Jewish General Hospital, Montreal, Quebec, Canada


    A method is described in which high-speed centrifugation of membranes through an oil phase is used to separate membrane-bound and detergent-solubilized polypeptide receptor-iodinated ligand complexes from unbound ligands. Three centrifuges, the Brinkmann Eppendorf (5412), the Beckman Microfuge B and the Beckman Airfuge were evaluated for this capability. Under the conditions described, the Beckman Airfuge surpassed the others in recovering previously /sup 125/I- and /sup 32/P-labelled cell membranes. The Airfuge method was compared with the more classically employed membrane filtration method to measure specific (/sup 125/I)insulin and (/sup 125/I)thrombin binding to human placental membranes and an enriched plasma membrane fraction from mouse embryo fibroblasts, respectively, and found to be 4 to 5 times more sensitive. For example, specific binding of ligand to its receptor was demonstrated with 5 of protein. With slight modifications, the polyethyleneglycol 6000 method of precipitating /sup 125/I-labelled ligand-soluble receptor complexes can be adapted to the Airfuge sedimentation through oil procedure.

  15. Seed drill depth control system for precision seeding

    DEFF Research Database (Denmark)

    Kirkegaard Nielsen, Søren; Munkholm, Lars Juhl; Lamandé, Mathieu


    acting on the drill coulters, which generates unwanted vibrations and, consequently, a non-uniform seed placement. Therefore, a proof-of-concept dynamic coulter depth control system for a low-cost seed drill was developed and studied in a field experiment. The performance of the active control system...... depth control system this variability was reduced to±2 mm. The system with the active control system operated more accurately at an operational speed of 12 km h−1 than at 4 km h−1 without the activated control system.......An adequate and uniform seeding depth is crucial for the homogeneous development of a crop, as it affects time of emergence and germination rate. The considerable depth variations observed during seeding operations - even for modern seed drills - are mainly caused by variability in soil resistance...

  16. Effect of Food, Diet and Nutrition on Military Readiness and Preparedness of Army Personnel and Dependents in a Peacetime Environment (United States)


    Analyzer, Volume 11, No.1, Coulter Electronics, Inc., 1989. 9. Schuster R. Determination of amino acids in biological, pharmaceutical, plant and food samples...spectroscopy. Atomic Absorption 1985; 6:56-59. 14. Pyrochemiluminescent nitrogen system: total urinary nitrogen procedure for in vitro diagnostic use. Antek... carnivore (leopard), an herbivore (rhinoceros), and an omnivore (man). The data from these studies suggest that urinary levels of CHP is higher in animals

  17. Ultra-Fast Low Concentration Detection of Candida Pathogens Utilizing High Resolution Micropore Chips


    Mulero, Rafael; Lee, Dong Heun; Kutzler, Michele A.; Jacobson, Jeffrey M.; Kim, Min Jun


    Although Candida species are the fourth most common cause of nosocomial blood stream infections in the United States, early diagnostic tools for invasive candidemia are lacking. Due to an increasing rate of candidemia, a new screening system is needed to detect the Candida species in a timely manner. Here we describe a novel method of detection using a solid-state micro-scale pore similar to the operational principles of a Coulter counter. With a steady electrolyte current flowing through the...

  18. Physical properties of five grain dust types.


    Parnell, C B; Jones, D D; Rutherford, R D; Goforth, K J


    Physical properties of grain dust derived from five grain types (soybean, rice, corn, wheat, and sorghum) were measured and reported. The grain dusts were obtained from dust collection systems of terminal grain handling facilities and were assumed to be representative of grain dust generated during the handling process. The physical properties reported were as follows: particle size distributions and surface area measurements using a Coulter Counter Model TAII; percent dust fractions less tha...

  19. Evaluation of three methods for hemoglobin measurement in a blood donor setting

    Directory of Open Access Journals (Sweden)

    Jacob Rosenblit


    Full Text Available CONTEXT: The hemoglobin (Hb level is the most-used parameter for screening blood donors for the presence of anemia, one of the most-used methods for measuring Hb levels is based on photometric detection of cyanmetahemoglobin, as an alternative to this technology, HemoCue has developed a photometric method based on the determination of azide metahemoglobin. OBJECTIVE: To evaluate the performance of three methods for hemoglobin (Hb determination in a blood bank setting. DESIGN: Prospective study utilizing blood samples to compare methods for Hb determination. SETTING: Hemotherapy Service of the Hospital Israelita Albert Einstein, a private institution in the tertiary health care system. SAMPLE: Serial blood samples were collected from 259 individuals during the period from March to June 1996. MAIN MEASUREMENTS: Test performances and their comparisons were assessed by the analysis of coefficients of variation (CV, linear regression and mean differences. RESULTS: The CV for the three methods were: Coulter 0.68%, Cobas 0.82% and HemoCue 0.69%. There was no difference between the mean Hb determination for the three methods (p>0.05. The Coulter and Cobas methods showed the best agreement and the HemoCue method gave a lower Hb determination when compared to both the Coulter and Cobas methods. However, pairs of methods involving the HemoCue seem to have narrower limits of agreement (± 0.78 and ± 1.02 than the Coulter and Cobas combination (± 1.13. CONCLUSION: The three methods provide good agreement for hemoglobin determination.

  20. Demonstration of an In-Situ Friction-Sound Probe for Mapping Particle Size at Contaminated Sediment Sites (United States)


    sedimentation according to the Stokes Law through pipettes (Plumb, 1981) or hydrometer (ASTM, 1988). Various optical techniques, electro-resistance (Coulter...used by the contracting laboratory (ASTM, 1998) is a combined mechanical sieving and hydrometer method. The sieves are used to quantify particle the sand range (> 63 µm) and larger, and a sedimentation- hydrometer technique is used to quantify the silt and clay size ranges (< 63 µm

  1. Characterization of Particles in Protein Solutions: Reaching the Limits of Current Technologies


    Demeule, Barth?lemy; Messick, Steven; Shire, Steven J.; Liu, Jun


    Recent publications have emphasized the lack of characterization methods available for protein particles in a size range comprised between 0.1 and 10??m and the potential risk of immunogenicity associated with such particles. In the present paper, we have investigated the performance of light obscuration, flow microscopy, and Coulter counter instruments for particle counting and sizing in protein formulations. We focused on particles 2?10??m in diameter and studied the effect of silicon oil d...

  2. Analytical and clinical performance of thyroglobulin autoantibody assays in thyroid cancer follow-up. (United States)

    Katrangi, Waddah; Grebe, Stephan K G; Algeciras-Schimnich, Alicia


    While thyroglobulin autoantibodies (TgAb) can result in false low serum thyroglobulin (Tg) immunoassay (IA) measurements, they might also be indicators of disease persistence/recurrence. Hence, accurate TgAb measurement, in addition to Tg quantification, is crucial for thyroid cancer monitoring. We compared the analytical and clinical performance of four commonly used TgAb IAs. We measured Tg by mass spectrometry (Tg-MS) and by four pairs of Tg and TgAb IAs (Beckman, Roche, Siemens, Thermo) in 576 samples. Limit of quantitation (LOQ) and manufacturers' upper reference interval cut-off (URI) were used for comparisons. Clinical performance was assessed by receiving operator characteristics (ROC) curve analysis. Quantitative and qualitative agreement between TgAb-IAs was moderate with R2 of 0.20-0.70 and κ from 0.41-0.66 using LOQ and 0.47-0.71 using URI. In samples with TgAb interference, detection rates of TgAb were similar using LOQ and URI for Beckman, Siemens, and Thermo, but much lower for the Roche TgAb-IA when the URI was used. In TgAb positive cases, the ROC areas under the curve (AUC) for the TgAb-IAs were 0.59 (Beckman), 0.62 (Siemens), 0.59 (Roche), and 0.59 (Thermo), similar to ROC AUCs achieved with Tg. Combining Tg and TgAb measurements improved the ROC AUCs compared to Tg or TgAb alone. TgAb-IAs show significant qualitative and quantitative differences. For 2 of the 4 TgAb-IAs, using the LOQ improves the detection of interfering TgAbs. All assays showed suboptimal clinical performance when used as surrogate markers of disease, with modest improvements when Tg and TgAb were combined.

  3. A comparison of analytical methods for detection of [14C]trichloro acetic acid-derived radioactivity in needles and branches of spruce (Picea sp.)

    International Nuclear Information System (INIS)

    Kretzschmar, M.; Matucha, M.; Uhlirova, H.


    The branches (wood and needles) of spruces of varying age treated with [ 14 C]trichloro acetic acid (3.7 GBq/mmol) were studied, using the following methods: Qualitative: - Conventional macroautoradiography with X-ray film and histological classification. Quantitative: - 14 C combustion analysis with the sample oxidizer A 307 (Canberra/Packard) followed by measurement of radioactivity using the LS counter 6000 (Beckman Instrumentts); - digital autoradiography with the Digital Autoradiograph LB 286 (Berthold GmbH); -digital autoradiography with the Bio-imaging Analyzer BAS 2000 (Fuji Film Co.). (orig.)

  4. Solar energy in the United States

    International Nuclear Information System (INIS)

    Ochoa, D.; Slaoui, A.; Soler, R.; Bermudez, V.


    Written by a group of five French experts who visited several research centres, innovating companies and solar power stations in the United States, this report first proposes an overview of solar energy in the United States, indicating and commenting the respective shares of different renewable energies in the production, focusing on the photovoltaic energy production and its RD sector. The second part presents industrial and research activities in the solar sector, and more specifically photovoltaic technologies (silicon and thin layer technology) and solar concentrators (thermal solar concentrators, photovoltaic concentrators). The last chapter presents the academic research activities in different universities (California Tech Beckman Institute, Stanford, National Renewable Energy Laboratory, Colorado School of Mines)

  5. Determination of average molecular weights on organic reactor coolants. II.-Freezing point depression method for diphenyl-ether solutions

    International Nuclear Information System (INIS)

    Carreira, M.


    In order to reduce limitations of solubility, the cryoscopic method developed for benzene solutions of polyphenyl mixtures has been extended to diphenyl-ether solutions by introducing some modifications imposed by the physico-chemical properties of this solvent. The Nernsto theory of Beckman's method has been revised, taking into account the heat-transfer characteristics of the system, and the results of that analysis have been used to fix upon the design parameters of a cryoscopic apparatus for measurements on diphenyl-ether solutions. (Author) 9 refs

  6. A designated centre for people with disabilities operated by St John of God Community Services Limited, Louth

    LENUS (Irish Health Repository)

    Byrne, Brendan


    Commercially available prolactin immunoassays detect macroprolactin to variable degrees. Best practice requires laboratories to assess the cross-reactivity of their prolactin assay with macroprolactin, and where appropriate, introduce a screen for the presence of macroprolactin. Our policy has been to reanalyse hyperprolactinaemic samples following polyethylene glycol (PEG) precipitation and to report the resultant value as the monomeric prolactin content of the sample. The goal of this study was to determine the need to continue PEG precipitation when prolactin measurements with the Wallac AutoDELFIA were replaced by the Beckman DxI 800.

  7. Determination of average molecular weights on organic reactor coolants. II.-Freezing point depression method for diphenyl-ether solutions; Determinacion de masas moleculares medias en refrigerantes nucleares organicos. II.- Crioscopia de disoluciones en eter difenilico

    Energy Technology Data Exchange (ETDEWEB)

    Carreira, M


    In order to reduce limitations of solubility, the cryoscopic method developed for benzene solutions of polyphenyl mixtures has been extended to diphenyl-ether solutions by introducing some modifications imposed by the physico-chemical properties of this solvent. The Nernsto theory of Beckman's method has been revised, taking into account the heat-transfer characteristics of the system, and the results of that analysis have been used to fix upon the design parameters of a cryoscopic apparatus for measurements on diphenyl-ether solutions. (Author) 9 refs.

  8. Determination of average molecular weights on organic reactor coolants. I.- Freezing-point depression method for benzene solutions; Determinaciond e masas moleculares medias en refrigerantes nucleares organicos. I.- Crioscopia de disolucion bencenicas

    Energy Technology Data Exchange (ETDEWEB)

    Carreira, M


    As a working method for determination of changes in molecular mass that may occur by irradiation (pyrolytic-radiolytic decomposition) of polyphenyl reactor coolants, a cryoscopic technique has been developed which associated the basic simplicity of Beckman's method with some experimental refinements taken out of the equilibrium methods. A total of 18 runs were made on samples of napthalene, biphenyl, and the commercial mixtures OM-2 (Progil) and Santowax-R (Monsanto), with an average deviation from the theoretical molecular mass of 0.6%. (Author) 7 refs.

  9. Determination of average molecular weights on organic reactor coolants. I.- Freezing-point depression method for benzene solutions

    International Nuclear Information System (INIS)

    Carreira, M.


    As a working method for determination of changes in molecular mass that may occur by irradiation (pyrolytic-radiolytic decomposition) of polyphenyl reactor coolants, a cryoscopic technique has been developed which associated the basic simplicity of Beckman's method with some experimental refinements taken out of the equilibrium methods. A total of 18 runs were made on samples of napthalene, biphenyl, and the commercial mixtures OM-2 (Progil) and Santowax-R (Monsanto), with an average deviation from the theoretical molecular mass of 0.6%. (Author) 7 refs

  10. Determination of average molecular weights on organic reactor coolants. I.- Freezing-point depression method for benzene solutions; Determinaciond e masas moleculares medias en refrigerantes nucleares organicos. I.- Crioscopia de disolucion bencenicas

    Energy Technology Data Exchange (ETDEWEB)

    Carreira, M.


    As a working method for determination of changes in molecular mass that may occur by irradiation (pyrolytic-radiolytic decomposition) of polyphenyl reactor coolants, a cryoscopic technique has been developed which associated the basic simplicity of Beckman's method with some experimental refinements taken out of the equilibrium methods. A total of 18 runs were made on samples of napthalene, biphenyl, and the commercial mixtures OM-2 (Progil) and Santowax-R (Monsanto), with an average deviation from the theoretical molecular mass of 0.6%. (Author) 7 refs.

  11. Determination of average molecular weights on organic reactor coolants. II.-Freezing point depression method for diphenyl-ether solutions; Determinacion de masas moleculares medias en refrigerantes nucleares organicos. II.- Crioscopia de disoluciones en eter difenilico

    Energy Technology Data Exchange (ETDEWEB)

    Carreira, M.


    In order to reduce limitations of solubility, the cryoscopic method developed for benzene solutions of polyphenyl mixtures has been extended to diphenyl-ether solutions by introducing some modifications imposed by the physico-chemical properties of this solvent. The Nernsto theory of Beckman's method has been revised, taking into account the heat-transfer characteristics of the system, and the results of that analysis have been used to fix upon the design parameters of a cryoscopic apparatus for measurements on diphenyl-ether solutions. (Author) 9 refs.

  12. Studies of microparticles in patients with the antiphospholipid syndrome (APS). (United States)

    Vikerfors, A; Mobarrez, F; Bremme, K; Holmström, M; Ågren, A; Eelde, A; Bruzelius, M; Antovic, A; Wallén, H; Svenungsson, E


    To study circulating platelet, monocyte and endothelial microparticles (PMPs, MMPs and EMPs) in patients with antiphospholipid syndrome (APS) in comparison with healthy controls. Fifty-two patients with APS and 52 healthy controls were investigated. MPs were measured on a flow cytometer (Beckman Gallios) and defined as particles sized APS patients versus controls (p APS patients. We observed a high number of EMPs expressing TF in APS patients. The numbers of MMPs and total EMPs were also higher as compared with healthy controls but in contrast to previous reports, the number of PMPs did not differ between groups.

  13. Estimation of particle size distribution in Athabasca oil sands by indirect neutron activation analysis

    Energy Technology Data Exchange (ETDEWEB)

    Schutte, R.; Thompson, G.R.; Donkor, K.K. [New Caledonia College, Prince George, BC (Canada). Dept. of Chemistry; Duke, M.J.M. [Alberta Univ., Edmonton, AB (Canada). SLOWPOKE Nuclear Reactor Facility; Cowles, R. [Syncrude Canada, Edmonton, AB (Canada); Li, X.P.; Kratochvil, B. [Alberta Univ., Edmonton, AB (Canada). Dept. of Chemistry


    Knowledge concerning the particle size distribution (PSD) of oil sands is necessary for optimal extraction of bitumen from the sand, and it indicates ore quality, gives a measure of process performance during bitumen extraction, and yields information useful for tailings management. Oil sands with mainly coarse particulates are usually bitumen rich and easy to process in the conventional hot water extraction process. These ores do not require the addition of sodium hydroxide as a process aid, and tailings volumes are minimal in contrast to high fines oil sands. Compared to the methods currently in use for determining the PSD in the oil sand industry, a method is described that is rapid, simple to carry out, and does not involve the use of organic solvents with attendant disposal problems. The principle behind the method is the development of a set of correlations by applying regression analysis to a large set of PSD and elemental analysis data. Predicted PSDs compare favorably with results obtained by existing methods. Each of the three PSD methods currently in use could be simulated by the INAA method. The INAA-based model that predicts hydrometer equivalent data was only applicable above certain lower limits for the amount of the fine size fractions present because of the limited sensitivity of the hydrometer method for PSD determination of fine fractions. For all six particle sizes studied, the INAA model had lower overall uncertainty than the corresponding Microtrac and Coulter instrument methods; the instrument repeatability of the INAA fell between those of Microtrac and Coulter. For Athabasca oil sands, the INAA-based method for PSD determination at and below 44 microm afforded results comparable to current Microtrac and Coulter methods. 13 refs., 9 tabs., 2 figs.

  14. Geochemical variability of copper and iron in Oman margin sediments

    Digital Repository Service at National Institute of Oceanography (India)

    Alagarsamy, R.

    ] below the oxygen minimum zone (OMZ) during RRS Discovery cruise 211/94 (Fig.1; Table 1). Sediment samples were immediately frozen (-20 ˚ C) on board prior to transport to the laboratory where they were freeze-dried and stored at -20 ˚ C prior... in the laboratory. 2.1. Analytical Methods Sediment grain size distribution in each section of cores collected below the OMZ was determined with a Coulter Counter (Model LS 130). Water content in each section of cores collected below the OMZ was determined...

  15. Physical properties of five grain dust types. (United States)

    Parnell, C B; Jones, D D; Rutherford, R D; Goforth, K J


    Physical properties of grain dust derived from five grain types (soybean, rice, corn, wheat, and sorghum) were measured and reported. The grain dusts were obtained from dust collection systems of terminal grain handling facilities and were assumed to be representative of grain dust generated during the handling process. The physical properties reported were as follows: particle size distributions and surface area measurements using a Coulter Counter Model TAII; percent dust fractions less than 100 micron of whole dust; bulk density; particle density; and ash content. PMID:3709482

  16. Interaction of erythrocytes and hexavalent uranium compounds -an autoanalytical study

    International Nuclear Information System (INIS)

    Stuart, W.I.; Shying, M.E.


    An automated analytical system was devised to measure the kinetics of hemolysis by uranyl compounds. Accurate plots of percentage hemolysis v. time were obtained; these, together with the corresponding differential curves, show that hemolysis of plasma-free erythrocytes is a two-stage process. The first stage of hemolysis is particularly affected by pH and anion content of uranyl solutions, and also by incubation of cell suspensions at 37 deg. before mixing with lysing solution. Complementary studies involving Coulter counting and microscopic observation established the general pattern of hemolysis and showed that cell agglutination is a prominent feature of the interaction of cells with uranyl solutions

  17. Analysis of images of acute human and animal leukaemia

    International Nuclear Information System (INIS)

    Feinermann, Emmanuel


    This research thesis first proposes a review of the development of stereology: historical backgrounds, basic principles. It discusses the choices regarding instrumentation: Coulter counter (principle and theory), quantitative analysis of particles, image analyser (optical microscope, epidiascope, scanners, detection, electronic pencil, computers, programming and data processing system), and stereo-logical parameters. The author then reports the stereo-logical study of acute human leukaemia: definition, classification, determination of spherical particle size distribution, lympho-blast size distributions. He reports the comparative study of rat L 5222 leukaemia and Brown Norway rat acute myelocytic leukaemia, and discusses their relationship with acute human leukaemia

  18. Patrones ferrocin??ticos en la beta-talasemia minor


    P??rez Blanco, Francisco Jos??; Jim??nez Jim??nez, M. J.; Mart??n Fern??ndez, A.


    Objetivos: Estudiar los par??metros del metabolismo del hierro en la beta-talasemia minor. Casu??stica y M??todos: Se estudian 58 sujetos (30 hombre y 28 mujeres) de edades entre 18-72 a??os diagnosticados de talasemia beta minor. Como controles se estudiaron 30 hombres y 28 mujeres del mismo espectro de edad. El estudio hematol??gico se realiz?? en el Coulter Counter, el bioqu??mico en el autoanalizador Hitachi, la hemoglobina A2 mediante electroforesis en celogel. El hierro m...

  19. Effect of successful 131I treatment on the peripheral blood picture in hyperthyroid patients

    International Nuclear Information System (INIS)

    Li Xiaoping; He Yunnan; Hu Qingwu


    Objective: To investigate the effect of successful 131 I therapy on the levels peripheral blood picture in hyperthyroid patients. Methods: Serum T 3 , T 4 , TSH (with ACCESS microparticle chemiluminescence immunoassay) and blood Hb, RBC, WBC and DC, Plt (with COULTER three assortments) counts were determined in 110 controls and 210 hyperthyroid patients both before and after 131 I therapy. Results: 131 I treatment of hyperthyroidism in this group of patients was very successful (P 131 I therapy. Conclusion: The application of 131 I to treat hyperthyroidism was very successful with no remarkable effect on peripheral blood picture. (authors)

  20. Left-wing choices for the century of energy; Linkse keuzes voor de eeuw van de energie

    Energy Technology Data Exchange (ETDEWEB)

    Samsom, D.; Van Est, R.; Verbong, G.; Zwerver, S.; Jansen, F.; Sluijter, F.W.; Wiersma, J.M.; Reehuis, G.


    In this issue of the magazine of the Wiardi Beckman Foundation (the scientific office for the social-democratic party in the Netherlands, the PvdA) five articles are dedicated to complex energy problems (e.g. energy supply security, climatic change) from the perspective of the social democrats. The titles of the contributions are: (1) Energy responsibility. Sustainable, reliable, democratic; (2) Stop the wind turbines; (3) No fear for nuclear power plants; (4) Energy in the polder mix; and (5) Energy is not the only connection between Europe and the Russian Federation. [Dutch] In deze aflevering van het tijdschrift van de Wiardi Beckman Stichting (het wetenschappelijk bureau van de Partij van de Arbeid) wordt in vijf artikelen vanuit een sociaal-democratisch perspectief gekeken naar de wijze waarop het complexe energievraagstuk, van voorzieningszekerheid en klimaat, het hoofd kan worden geboden. De titels van de bijdragen zijn (1) Verantwoorde energie: duurzaam, degelijk, democratisch; (2) Stop de windmolens; Geen angst voor kerncentrales; Energie in de poldermix; en Energie is niet het enige dat Rusland en Europa bindt.

  1. Evaluation of a capillary zone electrophoresis system versus a conventional agarose gel system for routine serum protein separation and monoclonal component typing. (United States)

    Roudiere, L; Boularan, A M; Bonardet, A; Vallat, C; Cristol, J P; Dupuy, A M


    Capillary zone electrophoresis of serum proteins is increasingly gaining impact in clinical laboratories. During 2003, we compared the fully automated capillary electrophoresis (CE) system from Beckman (Paragon CZE 2000) with the method agarose gel electrophoresis Sebia (Hydrasis-Hyris, AGE). This new study focused on the evaluation of analytical performance and a comparison including 115 fresh routine samples (group A) and a series of 97 frozen pathologic sera with suspicion of monoclonal protein (group B). Coefficients of variation (CVs %) for the five classical protein fractions have been reported to be consistenly serum samples (group B), there were 90 in which we detected a monoclonal protein by immunofixation (IF) (immunosubtraction (IS) was not used). AGE and Paragon 2000 failed to detect 7 and 12 monoclonal proteins, respectively, leading to a concordance to 92% for AGE and 87% for Paragon 2000 for identifying electrophoretic abnormalities in this group. Beta-globulin abnormalities and M paraprotein were well detected with Paragon 2000. Only 81% (21 vs 26) of the gammopathies were immunotyped with IS by two readers blinded to the IF immunotype. The Paragon 2000 is a reliable alternative to conventional agarose gel electrophoresis combining the advantages of full automation (rapidity, ease of use and cost) with high analytical performance. Qualified interpretation of results requires an adaptation period which could further improve concordance between the methods. Recently, this CE system has been improved by the manufacturer (Beckman) concerning the migration buffer and detection of beta-globulin abnormalities.

  2. Is automated platelet counting still a problem in thrombocytopenic blood?

    Directory of Open Access Journals (Sweden)

    Raimundo Antônio Gomes Oliveira

    Full Text Available CONTEXT: Reliable platelet counting is crucial for indicating prophylactic platelet transfusion in thrombocytopenic patients. OBJECTIVE: To evaluate the precision and accuracy of platelet counting for thrombocytopenic patients, using four different automated counters in comparison with the Brecher & Cronkite reference method recommended by the International Committee for Standardization in Hematology (ICSH. TYPE OF STUDY: Automated platelet counting assessment in thrombocytopenic patients. SETTING: Hematology Laboratory, Hospital do Servidor Público Estadual de São Paulo, and the Hematology Division of Instituto Adolfo Lutz, São Paulo, SP, Brazil. MAIN MEASUREMENTS: Brecher & Cronkite reference method and four different automated platelet counters. PARTICIPANTS: 43 thrombocytopenic patients with platelet counts of less than 30,000/µl RESULTS: The ADVIA-120 (Bayer, Coulter STKS, H1 System (Technicom-Bayer and Coulter T-890 automatic instruments presented great precision and accuracy in relation to laboratory thrombocytopenic samples obtained by diluting blood from normal donors. However, when thrombocytopenic patients were investigated, all the counters except ADVIA (which is based on volume and refraction index showed low accuracy when compared to the Brecher & Cronkite reference method (ICSH. The ADVIA counter showed high correlation (r = 0.947. However, all counters showed flags in thrombocytopenic samples. CONCLUSION: The Brecher & Cronkite reference method should always be indicated in thrombocytopenic patients for platelet counts below 30,000 plt /µl obtained in one dimensional counters.

  3. Reaching out to the American middle class: Peggy Noonan’s conservatism

    Directory of Open Access Journals (Sweden)



    Full Text Available Parmi les idéologues qui popularisent les idées conservatrices auprès du grand public, Peggy Noonan occupe une place intéressante. Moins extrémiste que Rush Limbaugh ou Ann Coulter, elle exprime les angoisses et les espoirs des Reagan Democrats, ces Américains moyens devenus conservateurs depuis les années 80. Cet article analyse les ambivalences d’un discours qui entre en résonance avec celles d’une partie de la classe moyenne américaine.Since the 1990s, Peggy Noonan’s writings have translated conservative ideas into articles and bestselling books for the wider American public. In a less abrasive way than Rush Limbaugh or Ann Coulter, she expresses the hopes and fears of the Reagan Democrats. This article analyzes Noonan’s ambivalent rhetoric, which mirrors the ambivalent attitudes of parts of America’s embattled middle class.

  4. Quantitation of specific myeloid cells in rat bone marrow measured by in vitro /sup 35/S-sulphate incorporation

    Energy Technology Data Exchange (ETDEWEB)

    Wright, A F; Rose, M S


    A biochemical measurement which can be used for quantitation of specific early myeloid cells in rat bone marrow has been developed. This measurement consists of a rapid, simple assay for the in vitro quantitation of /sup 35/S-sulfate incorporation into rat bone marrow cells. Incubation of bone marrow cells with /sup 35/S-sulfate led to a time-dependent increase in radioactivity obtained in perchloric acid insoluble fractions of bone marrow cell suspensions. This incorporation was inhibited by cyanide and puromycin. Autoradiography has demonstrated the radiolabel to be specifically associated with immature cells of the myeloid series. The cells most active in this respect were eosinophils. When rats were treated with endotoxin, the rate of /sup 35/S-sulfate incorporation was increased. Cell number measurements, using conventional histopathology and a Coulter Counter, demonstrated that endotoxin caused an initial release of mature granulocytes from the bone marrow. The regeneration of this mature population in the marrow was rapid, and was characterized by an increase in the number of immature cells and a concomitant increase in the rate of /sup 35/S-sulfate incorporation measured in preparations of bone marrow cells in vitro. Furthermore, this response to endotoxin has demonstrated that Coulter Counting techniques can be used to distinguish specific populations of cells (e.g. mature granulocytes) within the bone marrow.

  5. Partial sequence determination of metabolically labeled radioactive proteins and peptides

    International Nuclear Information System (INIS)

    Anderson, C.W.


    The author has used the sequence analysis of radioactive proteins and peptides to approach several problems during the past few years. They, in collaboration with others, have mapped precisely several adenovirus proteins with respect to the nucleotide sequence of the adenovirus genome; identified hitherto missed proteins encoded by bacteriophage MS2 and by simian virus 40; analyzed the aminoterminal maturation of several virus proteins; determined the cleavage sites for processing of the poliovirus polyprotein; and analyzed the mechanism of frameshifting by excess normal tRNAs during cell-free protein synthesis. This chapter is designed to aid those without prior experience at protein sequence determinations. It is based primarily on the experience gained in the studies cited above, which made use of the Beckman 890 series automated protein sequencers

  6. Applicability of a prototype for determination of absorbed dose using brachytherapy equipment with Ir-192 sources; Aplicabilidade de um prototipo para determinacao da dose absorvida utilizando equipamentos de braquiterapia com fontes de IR-192

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Vivianne Lucia Bormann; Almeida, Mayara Gabriella Oliveira de; Vieira, Rafaela Etelvina de Amorim; Silva, Waldecy Ananias da; Nascimento, Rizia Keila, E-mail:, E-mail:, E-mail:, E-mail:, E-mail: [Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil)


    This work aims at the development and improvement of a device to perform the absolute dosimetry sources of Ir-192 using the Fricke solution contained in a flask. The Fricke solution used was prepared using amounts of ferrous ammonium sulfate, sodium chloride and sulfuric acid, diluted with water tri distilled pre-established in the literature. The spectrophotometer used was a UV-VIS spectrophotometer (Beckman DU-640 Counter) for measuring the optical density at wavelength 304 nm. The calculation for determining the radial dose takes into account the radial distance and the angle formed with the transverse axis of the source. As the results obtained can be seen that the states of Pernambuco, Ceara, Paraiba e Piaui are in accordance with the recommendations of international standards of the International Atomic Energy Agency (IAEA), which considers not acceptable a difference greater than 5% of prescribed dose and measured dose.

  7. Applicability of a prototype for determination of absorbed dose using brachytherapy equipment with Ir-192 sources

    International Nuclear Information System (INIS)

    Souza, Vivianne Lucia Bormann; Almeida, Mayara Gabriella Oliveira de; Vieira, Rafaela Etelvina de Amorim; Silva, Waldecy Ananias da; Nascimento, Rizia Keila


    This work aims at the development and improvement of a device to perform the absolute dosimetry sources of Ir-192 using the Fricke solution contained in a flask. The Fricke solution used was prepared using amounts of ferrous ammonium sulfate, sodium chloride and sulfuric acid, diluted with water tri distilled pre-established in the literature. The spectrophotometer used was a UV-VIS spectrophotometer (Beckman DU-640 Counter) for measuring the optical density at wavelength 304 nm. The calculation for determining the radial dose takes into account the radial distance and the angle formed with the transverse axis of the source. As the results obtained can be seen that the states of Pernambuco, Ceara, Paraiba e Piaui are in accordance with the recommendations of international standards of the International Atomic Energy Agency (IAEA), which considers not acceptable a difference greater than 5% of prescribed dose and measured dose

  8. An examination of the hexokinase method for serum glucose assay using external quality assessment data. (United States)

    Westwood, A; Bullock, D G; Whitehead, T P


    Hexokinase methods for serum glucose assay appeared to give slightly but consistently higher inter-laboratory coefficients of variation than all methods combined in the UK External Quality Assessment Scheme; their performance over a two-year period was therefore compared with that for three groups of glucose oxidase methods. This assessment showed no intrinsic inferiority in the hexokinase method. The greater variation may be due to the more heterogeneous group of instruments, particularly discrete analysers, on which the method is used. The Beckman Glucose Analyzer and Astra group (using a glucose oxidase method) showed the least inter-laboratory variability but also the lowest mean value. No comment is offered on the absolute accuracy of any of the methods.

  9. Discussing epigenetics in Southern California (United States)


    With the goal of discussing how epigenetic control and chromatin remodeling contribute to the various processes that lead to cellular plasticity and disease, this symposium marks the collaboration between the Institut National de la Santé et de la Recherche Médicale (INSERM) in France and the University of California, Irvine (UCI). Organized by Paolo Sassone-Corsi (UCI) and held at the Beckman Center of the National Academy of Sciences at the UCI campus December 15–16, 2011, this was the first of a series of international conferences on epigenetics dedicated to the scientific community in Southern California. The meeting also served as the official kick off for the newly formed Center for Epigenetics and Metabolism at the School of Medicine, UCI ( PMID:22414797

  10. Discussing epigenetics in Southern California: a report from the International Symposium on Epigenetic Control and Cellular Plasticity, UCI, December 15-16, 2011. (United States)

    Rattner, Barbara P


    With the goal of discussing how epigenetic control and chromatin remodeling contribute to the various processes that lead to cellular plasticity and disease, this symposium marks the collaboration between the Institut National de la Santé et de la Recherche Médicale (INSERM) in France and the University of California, Irvine (UCI). Organized by Paolo Sassone-Corsi (UCI) and held at the Beckman Center of the National Academy of Sciences at the UCI campus December 15-16, 2011, this was the first of a series of international conferences on epigenetics dedicated to the scientific community in Southern California. The meeting also served as the official kick off for the newly formed Center for Epigenetics and Metabolism at the School of Medicine, UCI (

  11. Mesure de la masse volumique des particules des eaux résiduaires urbaines : protocole et évaluation des incertitudes




    La connaissance de la masse volumique des particules des eaux résiduaires urbaines est intéressante notamment pour l'étude de leur traitement par décantation ainsi que des relations entre concentrations en matières en suspension et paramètres optiques (turbidimétrie).Une étude des différentes méthodes de mesure de la masse volumique appliquées aux eaux résiduaires a été effectuée par G. Chebbo (1992). Il en a conclu que le pycnomètre à gaz type Beckman est le seul appareil donnant satisfactio...

  12. “Fiz péssima escolha” na tomada de decisão


    Antonio Mendes Silva Filho


    Há uma frase (do Steve Beckman) que diz “você faz suas escolhas e suas escolhas fazem você.” Todo indivíduo é dotado de habilidades que formam suas competências e lhe permite decisões. Há dias atrás, uma frase de minhas leituras me chamou a atenção, “fiz péssima escolha”. Independente do contexto ao qual ela estava inserida, o que aqui se destaca e desperta atenção é: o que leva uma pessoa a dizer “fiz péssima escolha”? Será errar ou medo de errar algo ruim? Será o descarte a solução dessa “p...

  13. Influence of population selection on the 99th percentile reference value for cardiac troponin assays. (United States)

    Collinson, Paul O; Heung, Yen Ming; Gaze, David; Boa, Frances; Senior, Roxy; Christenson, Robert; Apple, Fred S


    We sought to determine the effect of patient selection on the 99th reference percentile of 2 sensitive and 1 high-sensitivity (hs) cardiac troponin assays in a well-defined reference population. Individuals>45 years old were randomly selected from 7 representative local community practices. Detailed information regarding the participants was collected via questionnaires. The healthy reference population was defined as individuals who had no history of vascular disease, hypertension, or heavy alcohol intake; were not receiving cardiac medication; and had blood pressure60 mL·min(-1)·(1.73 m2)(-1), and normal cardiac function according to results of echocardiography. Samples were stored at -70 °C until analysis for cardiac troponin I (cTnI) and cardiac troponin T (cTnT) and N-terminal pro-B-type natriuretic peptide. Application of progressively more stringent population selection strategies to the initial baseline population of 545 participants until the only individuals who remained were completely healthy according to the study criteria reduced the number of outliers seen and led to a progressive decrease in the 99th-percentile value obtained for the Roche hs-cTnT assay and the sensitive Beckman cTnI assay but not for the sensitive Siemens Ultra cTnI assay. Furthermore, a sex difference found in the baseline population for the hs-cTnT (P=0.0018) and Beckman cTnI assays (Pstrategy significantly influenced the 99th percentile reference values determined for troponin assays and the observed sex differences in troponin concentrations.

  14. Status of internal quality control for thyroid hormones immunoassays from 2011 to 2016 in China. (United States)

    Zhang, Shishi; Wang, Wei; Zhao, Haijian; He, Falin; Zhong, Kun; Yuan, Shuai; Wang, Zhiguo


    Internal quality control (IQC) plays a key role in the evaluation of precision performance in clinical laboratories. This report aims to present precision status of thyroid hormones immunoassays from 2011 to 2016 in China. Through Clinet-EQA reporting system, IQC information of Triiodothyronine and Thyroxine in the form of free and total (FT3, TT3, FT4, TT4), as well as Thyroid Stimulating Hormone (TSH) were collected from participant laboratories submitting IQC data in February, 2011-2016. For each analyte, current CVs were compared among different years and measurement systems. Percentages of laboratories meeting five allowable imprecision specifications (pass rates) were also calculated. Analysis of IQC practice was conducted to constitute a complete report. Current CVs were decreasing significantly but pass rates increasing only for FT3 during 6 years. FT3, TT3, FT4, and TT4 had the highest pass rates comparing with 1/3TEa imprecision specification but TSH had this comparing with minimum imprecision specification derived from biological variation. Constituent ratios of four mainstream measurement systems changed insignificantly. In 2016, precision performance of Abbott and Roche systems were better than Beckman and Siemens systems for all analytes except FT3 had Siemens also better than Beckman. Analysis of IQC practice demonstrated wide variation and great progress in aspects of IQC rules and control frequency. With change of IQC practice, only FT3 had precision performance improved in 6 years. However, precision status of five analytes in China was still unsatisfying. Ongoing investigation and improvement of IQC have yet to be achieved. © 2017 Wiley Periodicals, Inc.

  15. Secoisolariciresinol Diglucoside (SDG) Isolated from Flaxseed, an Alternative to ACE Inhibitors in the Treatment of Hypertension. (United States)

    Prasad, Kailash


    Secoisolariciresionol diglucoside (SDG) is a plant lignan isolated from flaxseed and is phytoestrogen. SDG is a potent and long-acting hypotensive agent. Plant phytoestrogens have inhibitory effects on angiotensin-converting enzyme (ACE). The hypotensive effects of SDG, a phytoestrogen, may be mediated through inhibition of ACE. The objective of this study was to investigate if SDG-induced hypotension is mediated through inhibition of ACE. The Sprague Dawley male rats were anesthetized and trachea was cannulated. The right jugular vein was cannulated to administer the drug and the carotid artery was cannulated to record arterial pressures using PIOEZ-1 miniature model transducer (Becton, Dickinson and Company, Franklin Lakes, NJ) and Beckman dynograph (Beckman Instruments, Inc., Schiller Park, IL). The effects of angiotensin I (0.2 µg/kg, intravenously [IV]) in the absence and presence of SDG (10 mg/kg, IV), and SDG alone on systolic, diastolic, and mean arterial pressures were measured before and after 15, 30, and 60 minutes of drug administration. SDG decreased the systolic, diastolic, and mean arterial pressure by 37, 47, and 43%, respectively, at 15 minutes and 18.8, 21.2, and 20.3%, respectively, at 60 minutes. Angiotensin I increased the arterial pressure. SDG decreased angiotensin I-induced rise in the systolic, diastolic, and mean arterial pressures by 60, 58, and 51%, respectively, at 15 minutes and 48, 46, and 30%, respectively, at 60 minutes. The data suggest that SDG reduced the angiotensin I-induced rise in the arterial pressures and hence SDG is a potent ACE inhibitor.

  16. Ultra-fast low concentration detection of Candida pathogens utilizing high resolution micropore chips. (United States)

    Mulero, Rafael; Lee, Dong Heun; Kutzler, Michele A; Jacobson, Jeffrey M; Kim, Min Jun


    Although Candida species are the fourth most common cause of nosocomial blood stream infections in the United States, early diagnostic tools for invasive candidemia are lacking. Due to an increasing rate of candidemia, a new screening system is needed to detect the Candida species in a timely manner. Here we describe a novel method of detection using a solid-state micro-scale pore similar to the operational principles of a Coulter counter. With a steady electrolyte current flowing through the pore, measurements are taken of changes in the current corresponding to the shape of individual yeasts as they translocate or travel through the pore. The direct ultra-fast low concentration electrical addressing of C. albicans has established criteria for distinguishing individual yeast based on their structural properties, which may reduce the currently used methods' complexity for both identification and quantification capabilities in mixed blood samples.

  17. Ultra-Fast Low Concentration Detection of Candida Pathogens Utilizing High Resolution Micropore Chips

    Directory of Open Access Journals (Sweden)


    Full Text Available Although Candida species are the fourth most common cause of nosocomial blood stream infections in the United States, early diagnostic tools for invasive candidemia are lacking. Due to an increasing rate of candidemia, a new screening system is needed to detect the Candida species in a timely manner. Here we describe a novel method of detection using a solid-state micro-scale pore similar to the operational principles of a Coulter counter. With a steady electrolyte current flowing through the pore, measurements are taken of changes in the current corresponding to the shape of individual yeasts as they translocate or travel through the pore. The direct ultra-fast low concentration electrical addressing of C. albicans has established criteria for distinguishing individual yeast based on their structural properties, which may reduce the currently used methods’ complexity for both identification and quantification capabilities in mixed blood samples

  18. Investigations of heavy ion tracks in polyethylene naphthalate films

    CERN Document Server

    Starosta, W; Sartowska, B; Buczkowski, M


    The heavy ion beam (with fluence 3x10 sup 8 ion/cm sup 2) from a cyclotron has been used for irradiation of thin polyethylene naphthalate (PEN) films. Latent tracks in these polymeric films have been sensitized by UV radiation and then chemically etched in NaOH solution. The etching process parameters have been controlled by the electroconductivity method. After etching, parameters of samples have been examined by SEM and bubble point methods (Coulter[reg] Porometer II instrument). Results have shown good quality of PEN track membranes with pore sizes in the range: 0.1 - 0.5 mu m. The described procedure is known for thin polyethylene terephthalate (PET) films. Taking into consideration that PEN films have got better mechanical, thermal, gas barrier as well as better chemical resistance properties in comparison with PET films, the possibility of application of such membranes is much wider.





    El hemograma es uno de los exámenes de laboratorio solicitado con mayor frecuencia y forma parte del estudio básico requerido para orientación diagnóstica y evaluación de los pacientes. La vigencia de este examen se ha mantenido desde la introducción de los clásicos índices eritrocitarios descritos por Wintrobe en los años 30, evolucionando con la automatización de los recuentos celulares desarrollada por Coulter en los años 50 y la incorporación de nuevos parámetros como amplitud de distr...

  20. Nuclear ploidy of neonatal rat livers: effects of two hepatic carcinogens (mirex and dimethylnitrosamine)

    International Nuclear Information System (INIS)

    Carlson, J.; Abraham, R.


    The effect of two hepatic carcinogens, dimethylnitrosamine (DMN) (genotoxic) and mirex (epigenetic), on polyploidization in 12-d-old neonatal rats was investigated by Coulter counteranalysis and [ 3 H] thymidine uptake in isolated hepatic nuclear classes. DMN disturbed the normal ploidy development in the neonatal liver and the proportion of nuclei in the ploidy classes by inducing the premature formation of a significant population of tetraploids with a concommitant reduction in diploids. A great proportion of the replicative activity was present in tetraploid nuclei as measured by the incorporation of [ 3 H] thymidine. The labeling index and number of mitoses were also increased. In contrast to DMN, mirex had no influence on polyploidization. The neonatal rats used in these studies thus offer an opportunity to investigate in vivo the mode of action of genotoxic versus epigenetic compounds with reference to their effect on DNA

  1. Photon correlation spectroscopy of classical and non-classical light fields and its debt to Glauber and Harvard

    International Nuclear Information System (INIS)

    Pike, E.R.


    The Union Gikon Company of Japan has designed their new photon-correlation spectrometer to the outside world with the aid of a number of Japanese Universities and Industrial Research Laboratories. It comes with a list of some two dozen Japanese Institutions who have been using it successfully since its recent launch in their home country. The cost of the system is some $60,000 and it will no doubt, find its place in the market alongside existing well-known photon correlation systems such as those from Brookhaven Instruments Corporation, Hiac Royco, Coulter and Malvern Instruments. Although they may be used for other purposes, the main application of the instruments is the measurement of sizes of submicron particles such as proteins, enzymes, viruses, polymers and numerous other macro-molecular substances. A topical proposal, for example, is the detection of the immunological reactions of the AIDS virus. Photon correlation spectroscopy has become an important technique in modern laboratory practice

  2. Vascular volumes and hematology in male and female runners and cyclists. (United States)

    Green, H J; Carter, S; Grant, S; Tupling, R; Coates, G; Ali, M


    To examine the hypothesis that foot-strike hemolysis alters vascular volumes and selected hematological properties is trained athletes, we have measured total blood volume (TBV), red cell volume (RCV) and plasma volume (PV) in cyclists (n = 21) and runners (n = 17) and compared them to those of untrained controls (n = 20). TBV (ml x kg(-1)) was calculated as the sum of RCV (ml x kg(-1)) and PV (ml x kg(-1)) obtained using 51Cr and 125I-labelled albumin, respectively. Hematological assessment was carried out using a Coulter counter. Peak aerobic power (VO2peak) was measured during progressive exercise to fatigue using both cycle and treadmill ergometry. RCV was 15% higher (P strike hemolysis would not appear to have an effect on that parameter. The significant correlations (P role for the vascular system in realizing a high aerobic power.

  3. Properties of PETP track membranes of different thickness

    International Nuclear Information System (INIS)

    Apel, P.Yu.; Didyk, A.Yu.; Larionova, I.E.; Mamonova, T.I.; Orelovich, O.L.; Samoilova, L.I.; Yanina, I.V.; Zhitariuk, N.I.


    The basic properties of polyethylene terephthalate (PETP) track membranes (TM) made of the films with the thickness of 10 and 20 μm are investigated. The membranes with the pore diameters of 0.2, 0.4 and 1 μm were chosen for comparative study. The porous structure of the membranes was characterized by the following set of parameters: pore density, pore size, bubble point, pore radius distribution measured by the Coulter porometer. The tensile strength, water and gas flow rate were determined for both types of the TMs. The filtration processes of liquid and gaseous media were investigated. Advantages and disadvantages of 'thin' and 'thick' TM are discussed. (author). 4 refs, 5 figs, 3 tabs

  4. Biomek®-3000 and GenPlex SNP Genotyping in Forensic Genetics

    DEFF Research Database (Denmark)

    Stangegaard, Michael; Tomas, Carmen; Hansen, Anders J.


    Single nucleotide polymorphism genotyping provides a supplement for conventional short tandem repeats-based kits currently used for human identification. GenPlex (Applied Biosystems (AB), Foster City, CA) is an SNP-genotyping kit based on a multiplex of 48 informative, autosomal SNPs from...... the SNPforID Consortium. Our objective was to setup, implement, and validate a small and affordable automated liquid-handling robot for forensic casework samples (buccal swaps on FTA-paper and Qiagen purified blood). The reaction scheme consisted of numerous steps and was cumbersome to perform consistently...... manually. Automation was accomplished with a Biomek-3000 (Beckmann Coulter) laboratory-automated workstation using five in-house-developed methods. All methods allowed the user to select the number of subsequent injections to the capillary electrophoresis instrument (ABI 3130xl, AB) enabling processing...

  5. Survival and activity of Streptococcus faecalis and Escherichia coli in tropical freshwater

    Energy Technology Data Exchange (ETDEWEB)

    Muniz, I.; Jimenez, L.; Toranzos, G.A.; Hazen, T.C. [Univ. of Puerto Rico, Rio Piedras (Puerto Rico)


    The survival of Streptococcus facecalis and Escherichia coli was studied in situ in a tropical rain forest watershed using membrane diffusion chambers. Densities were determined by acridine orange direct count and Coulter Counter. Population activity was determined by microautoradiography, cell respiration, and by nucleic acid composition. Densities of S. facecalis and E. coli decreased less than 1 log unit after 105 h as measured by direct count methods. Activity as measured by respiration, acridine orange activity, and microautoradiography indicated that both bacteria remained moderately active during the entire study. After 12 h, E. coli was more active than S. faecalis as measured by nucleic acid composition. E. coli and S. faecalis survived and remained active for more than 5 days. Consequently, both would seem to be unsuitable as indicators of recent fecal contamination in tropical waters.

  6. Reconnaissance surficial geologic map of the Taylor Mountains quadrangle, southwestern Alaska (United States)

    Wilson, Frederic H.


    This map and accompanying digital files are the result of the interpretation of aerial photographs from the 1950s as well as more modern imagery. The area, long considered a part of Alaska that was largely not glaciated (see Karlstrom, 1964; Coulter and others, 1965; or Péwé, 1975), actually has a long history reflecting local and more distant glaciations. An unpublished photogeologic map of the Taylor Mountains quadrangle from the 1950s by J.N. Platt Jr. was useful in the construction of this map. Limited new field mapping in the area was conducted as part of a mapping project in the Dillingham quadrangle to the south (Wilson and others, 2003); however, extensive aerial photograph interpretation represents the bulk of the mapping effort. The accompanying digital files show the sources for each line and geologic unit shown on the map.

  7. Improved tumor imaging with radiolabeled monoclonal antibodies by plasma clearance with anti-antibody column

    International Nuclear Information System (INIS)

    Lear, J.L.; Kasliwal, R.; Feyerabend, A.; Bunn, P.; Dienhart, D.G.; Johnson, T.K.; Glenn, S.D.; Maddock, S.W.


    This paper reports on imaging of tumors with use of radiolabeled monoclonal antibodies (MoAs) that often hindered by high levels of background activity. The ability to lower blood pool MoA activity at a selected time after injection offers a potential method to reduce background while preserving tumor uptake. Toward this goal, the authors investigated the process of clearing MoA from patients' plasma with use of an anti-antibody column. One patient with breast cancer and four with lung cancer were given intravenous injection of 5 mCi of indium-111 KC4 (Coulter Immunology) and imaged at 20, 24, 48, and 72 hours with use of a whole-body canner coupled to a computer. Plasma clearance was performed between the 20- and 24-hour images with use of a COBEIA system. Images were inspected visually and analyzed by region-of-interest quantification

  8. El liderazgo docente y su relación con el estilo gerencial de los pasantes de Comunicación Social de la Universidad del Zulia

    Directory of Open Access Journals (Sweden)

    Rosario Fonseca


    Full Text Available Se propuso determinar la relación entre el liderazgo docente y el estilo gerencial de los estudiantes de Comunicación Social, mención Periodismo Impreso de LUZ, de acuerdo a la clasificación de Robbins, Stephen y Coulter (1999, y Guédez (1996 y siguiendo una metodología descriptiva-correlacional. Se aplicó un cuestionario para docentes y estudiantes de los últimos semestres. Los resultados demuestran que existe relación entre el liderazgo de los docentes, en su mayoría de tipo Democrático-Situacional, y el estilo gerencial de los estudiantes, predominó el estilo Estratégico y Situacional.

  9. Survival and activity of Streptococcus faecalis and escherichia coli in tropical freshwater

    International Nuclear Information System (INIS)

    Muniz, I; Toranzos, G.A.; Jimenez, L.; Hazen, T.C.


    The survival of Streptococcus faecalis and Escherichia coli was studied in situ in a tropical rain forest watershed using membrane diffusion chambers. Densities were determined by acridine orange direct count and Coulter Counter. Population activity was determined by microautoradiography, cell respiration, and by nucleic acid composition. Densities of S. faecalis and E. coli decreased less than 1 log unit after 105 hours as measured by direct count methods. Activity as measured by respiration, acridine orange activity, and microautoradiography indicated that both bacteria remained moderately active during the entire study. After 12 hours, E. coli was more active than S. faecalis as measured by nucleic acid composition. In this tropical rain forest watershed, E. coli and S. faecalis survived and remained active for more than 5 days; consequently, both would seem to be unsuitable as indicators of recent fecal contamination in tropical waters

  10. Influence du temps de conservation du sang sur l'hémogramme réalisé avec le Vet-ABC chez le chien et le chat


    Caillard, Agnès


    L'utilisation du Vet abc, automate d'hématologie de type «coulter » a permis d'étudier les modifications engendrées par la conservation du sang pendant 24 heures à température ambiante, sur les paramètres et constituants de l'hémogramme du chien et du chat. L'évaluation de l'effet de la conservation du sang sur l'IDR et sur les courbes de distribution cellulaire, constitue l'originalité de ce travail. Les évolutions les plus notables ont été les suivantes : - un VGM qui augmente en moyenne de...

  11. Construction of an experimental plot seeder of wheat planting and compare it by imported one

    Directory of Open Access Journals (Sweden)

    I Eskandari


    Full Text Available Introduction Researchers frequently include multiple cultivars and fertility levels in field experiments. Therefore, the experiments sowing operation must represent a considerable saving in time and labor, compared to hand sowing. Greater flexibility in experimental design and setup could be achieved by equipment that enables quick changes in the cultivar and fertilizer rates from one plot to the next. A satisfactory seed drill must distribute a given quantity of seed evenly over a predetermined length of coulter row, the coulters must be spaced at exact intervals and depth of sowing must be uniform. In a self-propelled type of plot seeder, no coulter should run in a wheel track as the compaction of the soil can cause observable differences in vigor between plants in such a row and those in un-compacted rows. The machine should sow in succession from a try in which a series of seed pocket separated clearly and must be put into distributer funnel by an assistant operator. The length of gap being varied according to the nature and purpose of the plot. The objectives of this experiment were 1- to design and construct a local self-propelled plot seeder and 2- To compare it with the imported (Wintersteiger plot seeder in cereal breeding programs. Materials and Methods A small-plot seeder was designed and constructed to meet this objective. The unit consists of the following basic components: a toolbar for pulling a set of six blade coulter, an air compressor for lifting and putting down the openers and metering transmission drive wheel, an operators chair and work rack, one belt seed distribution. A cone-celled and rotor seed distributor is used for seed distribution to the openers. The cone system is connected to the gearbox and allows for great flexibility in changing cultivars, crop species, and plot length. This is driven by the separate drive wheel. The cone-celled distributor sows all the seed of the sample in making one complete turn. The

  12. Thyroglobulin (Tg) Testing Revisited: Tg Assays, TgAb Assays, and Correlation of Results With Clinical Outcomes. (United States)

    Netzel, Brian C; Grebe, Stefan K G; Carranza Leon, B Gisella; Castro, M Regina; Clark, Penelope M; Hoofnagle, Andrew N; Spencer, Carole A; Turcu, Adina F; Algeciras-Schimnich, Alicia


    Measurement of thyroglobulin (Tg) by mass spectrometry (Tg-MS) is emerging as a tool for accurate Tg quantification in patients with anti-Tg autoantibodies (TgAbs). The objective of the study was to perform analytical and clinical evaluations of two Tg-MS assays in comparison with immunometric Tg assays (Tg-IAs) and Tg RIAs (Tg-RIAs) in a cohort of thyroid cancer patients. A total of 589 samples from 495 patients, 243 TgAb-/252 TgAb+, were tested by Beckman, Roche, Siemens-Immulite, and Thermo-Brahms Tg and TgAb assays, two Tg-RIAs, and two Tg-MS assays. The frequency of TgAb+ was 58%, 41%, 27%, and 39% for Roche, Beckman, Siemens-Immulite, and Thermo-Brahms, respectively. In TgAb- samples, clinical sensitivities and specificities of 100% and 74%-100%, respectively, were observed across all assays. In TgAb+ samples, all Tg-IAs demonstrated assay-dependent Tg underestimation, ranging from 41% to 86%. In TgAb+ samples, the use of a common cutoff (0.5 ng/mL) for the Tg-MS, three Tg-IAs, and the USC-RIA improved the sensitivity for the Tg-MSs and Tg-RIAs when compared with the Tg-IAs. In up to 20% of TgAb+ cases, Tg-IAs failed to detect Tg that was detectable by Tg-MS. In Tg-RIAs false-high biases were observed in TgAb+ samples containing low Tg concentrations. Tg-IAs remain the method of choice for Tg quantitation in TgAb- patients. In TgAb+ patients with undetectable Tg by immunometric assay, the Tg-MS will detect Tg in up to 20% additional cases. The Tg-RIA will detect Tg in approximately 35% cases, but a significant proportion of these will be clinical false-positive results. The undetectable Tg-MS seen in approximately 40% of TgAb+ cases in patients with disease need further evaluation.

  13. Índices eritrocitarios en la esferocitosis hereditaria Erythrocyte indexes in hereditary spherocytosis

    Directory of Open Access Journals (Sweden)

    Silvia Eandi Eberle


    Full Text Available La esferocitosis hereditaria es un grupo heterogéneo de desórdenes caracterizados por la variabilidad en la clínica, en los defectos proteicos del citoesqueleto eritrocitario y en el tipo de herencia. Se estudió la sensibilidad y especificidad de la concentración de hemoglobina corpuscular media (CHCM y el índice de amplitud de distribución eritrocitaria (ADE en el screening diagnóstico de la esferocitosis hereditaria. Noventa y cuatro pacientes fueron comparados con niños sanos de igual sexo y edad. Todos los índices se obtuvieron por impedancia eléctrica (autoanalizador hematológico Coulter JT. En los pacientes con esferocitosis hereditaria, la CHCM (35.67±1.33 g/dl y el ADE (20.60±4.5%, fueron significativamente más elevados que en el grupo control (CHCM 33.48±0.68 g/dl, p 0.000; ADE 13.22±0.9%, p 0.000. Con los valores de corte utilizados en nuestro laboratorio (CHCM ≥ 34.5 g/dl; ADE ≥ 14.5% ambos índices elevados mostraron una sensibilidad de 81% y una especificidad de 98.9% en el screening de esferocitosis hereditaria. La combinación de ambos índices es un excelente predictor para el diagnóstico de esferocitosis hereditaria.Hereditary spherocytosis is a group of heterogenous disorders characterized by variability in its clinical manifestations, membrane protein defects and inheritance. We analysed the sensitivity and specificity of mean corpuscular hemoglobin concentration (MCHC and red cell distribution width (RDW in the diagnostic screening of hereditary spherocytosis. Ninetyfour patients were compared to equal number of healthy, age-matched children. All indexes were derived from measurements obtained by aperture impedance (Coulter Counter Model JT. In patients with hereditary spherocytosis, MCHC (35.67±1.33 g/dl and RDW (20.60±4.5% were significantly higher than in normal control subjects (MCHC 33.48±0.68 g/dl, p: 0.000; RDW 13.22±0.9%, p: 0.000. By using a cutoff for the MCHC of 34.5 g/dl and for the RDW

  14. Anemia em escolares da primeira série do ensino fundamental da rede pública de Maceió, Alagoas, Brasil Anemia in public school first graders in the city of Maceió, Alagoas, Brazil

    Directory of Open Access Journals (Sweden)

    Célia Dias dos Santos


    Full Text Available Realizou-se um estudo transversal com amostra probabilística de 426 alunos de 6 a 10 anos, da 1ª série do ensino fundamental das escolas públicas de Maceió, Alagoas, Brasil. O objetivo foi avaliar a prevalência da anemia e sua associação com o retardo de crescimento. Os dados foram coletados entre maio e julho de 2000. O sangue foi colhido por venipuntura e a dosagem de hemoglobina (Hb foi realizada pelo contador Coulter STKS. Classificou-se a anemia por dois critérios estabelecidos pela Organização Mundial da Saúde: Hb A cross-sectional study was conducted in a representative sample of 426 randomly selected first graders (ages 6 to 10 years from public schools in Maceió, State of Alagoas, Brazil. The aim was to determine the prevalence of anemia, as well as its association with growth retardation. Data were collected from May to July 2000, and determination of hemoglobin (HGB employed an STKS Coulter counter. Two cut-off points were used to classify anemia, both established by the World Health Organization: HGB < 11.5g/dl and HGB < 12.0g/dl. The indicators height/age (H/A, weight/age (W/A, and weight/height (W/H below -2.0 standard deviations from the NCHS reference were diagnosed as growth retardation. Prevalence of anemia was 9.9% when HGB < 11.5g/dl was used, and 25.4% when the cut-off point was HGB < 12.0g/dl. Growth retardation was detected in 6.2% of children according to H/A, 4.0% for W/A, and 3.0% for W/H. There was no statistically significant association between the variables in the study. These findings confirm results of previous surveys where prevalence of anemia was much higher than that of growth retardation. The severe consequences of anemia in this age group justify the implementation of broad public policies to overcome this nutritional deficiency.

  15. 糖尿病视网膜病变患者血小板参数与糖化血红蛋白变化及临床意义%Study on clinical significance of changes in MPV and HbAlc in patients with diabetic retinopathy.

    Institute of Scientific and Technical Information of China (English)

    徐威; 蔡应木; 王彩霞


    目的 探讨血小板平均体积(MPV)及糖化血红蛋白(HbA1c)在糖尿病视网膜病变时的变化及临床意义.方法采用BEACKMAN COULTER LH750 全自动血细胞分析仪和TOSOH HLC-723G8全自动糖化血红蛋白分析仪检测85例糖尿病患者[单纯糖尿病者45例,糖尿病视网膜病变(DR)者40例]的血小板计数(PLT)、MPV、血小板分布宽度(PDW)、HbA1c并与40例健康成人(对照组)进行对照比较.结果 DR组HbA1c、MPV、PDW明显高于单纯糖尿病组及对照组(P<0.05,P<0.01),而PLT低于单纯糖尿病组及对照组(P<0.05).结论 HbA1c与MPV的联合检测对糖尿病患者视网膜病变的发生及预后判断方面具有重要意义,有助于早期发现DR的存在.%Objective To explore the clinical significance of changes in MPV and glycosylated hemoglobin ( HbAlc ) in patients with diabetic retinopathy. Methods Blood platelet count, mean platelet volume ( MPV ) and platelet distribution width ( PDW ) of 45 diabetics without retinopathy, 40 diabetics with retinopathy and 40 healthy persons were measured by automatic blood cell analyzer BEACKMAN COULTER LH750. Level of HbAlc was measured by automatic glycosylated hemoglobin analyzer TOSOH HLC -723G8. Results Compared with healthy and diabetes groups, levels of HbAlc, MPV and PDW in diabetic retinopathy group were remarkably increased ( P<0.05, P<0. 01 ), while platelet count was decreased ( P<0.05 ). Conclusion Joint detection of level of HbAlc and MPV is helpful for early diagnosis and prognosis judgement of diabetic retinopathy in patients with diabetes.

  16. Mecanismos de abertura do sulco e adubação nitrogenada no cultivo do feijoeiro em sistema plantio direto Furrows opening mechanism for nitrogen fertilizer application in common bean crop under no-tillage

    Directory of Open Access Journals (Sweden)

    Orivaldo Arf


    Full Text Available Algumas culturas têm pouca adaptação ao sistema plantio direto, em vista da maior compactação da camada superficial do solo e, nesse caso, o mecanismo utilizado na semeadora para a abertura dos sulcos para deposição do fertilizante pode ter grande importância no sentido de facilitar a penetração das raízes. Este experimento foi desenvolvido em Selvíria (MS, com o objetivo de avaliar a produtividade do feijão de inverno cultivado em sistema plantio direto, em função da utilização de mecanismos de abertura para distribuição de fertilizantes na semeadura e da adubação nitrogenada em cobertura. O delineamento experimental utilizado foi em blocos casualizados, utilizando-se esquema fatorial 2 x 6, constituído por mecanismos de distribuição de fertilizante (haste escarificadora e disco duplo e doses de N em cobertura (0, 25, 50, 75, 100 e 125 kg ha-1, com quatro repetições. Recomenda-se o uso da haste escarificadora como mecanismo de distribuição do fertilizante, para o cultivo do feijoeiro de inverno. A adubação nitrogenada em cobertura proporciona incrementos à produtividade do feijoeiro de inverno.Some crops have shown no adaptation to no-tillage system as a function of compaction soil superficial layer. In way, the mechanism used in seeder to open furrows for deposition of fertilizer can have great importance to facilitate the penetration of roots. This experiment was carried in Selvíria (MS, with the objective to evaluate the winter common bean crop yield under no-tillage system, as function of fertilizer distribution opening mechanisms in sowing (chisel and coulter blade and sidedressing nitrogen application (0, 25, 50, 75, 100 and 125 kg ha-1. The experimental design was a randomized block, arranged in a 2 x 6 factorial scheme, constituted by fertilizer distribution opening mechanisms in sowing (chisel and coulter blade and sidedressing nitrogen doses (0, 25, 50, 75, 100 and 125 kg ha-1, with four replications

  17. Validación de un método de determinación del polisacárido B residual en vesículas de membrana externa de Neisseria meningitidis

    Directory of Open Access Journals (Sweden)

    Jannete Rico


    Full Text Available La Cromatografía Líquida de Alta Resolución-Fluorescencia ha resultado ser una herramienta sensible para la detección de trazas en productos farmacéuticos y es utilizada frecuentemente para la cuantificación de algunos polisacáridos de origen bacteriano que poseen ácido siálico como unidad repetitiva. En este trabajo nos planteamos como objetivo establecer y validar el método de HPLC_Fluorescencia, para determinar el contenido de polisacárido B residual en vesículas de membrana externa de Neisseria meningitidis. Para ello se realizó el montaje del método HPLC-FL, utilizando una columna de fase reversa C18 (Ultrasphere ODS, Beckman, USA y se evaluaron seis lotes de vesículas de membrana externa de diferentes cepas Cu 385/83 y NZ 228. Se demostró que este método permitió la cuantificación de polisacárido B de una forma específica y exacta, y que cumplía con todos los parámetros de validación establecidos para un método de determinación de trazas mediante HPLC.

  18. Failure rate analysis using GLIMMIX

    International Nuclear Information System (INIS)

    Moore, L.M.; Hemphill, G.M.; Martz, H.F.


    This paper illustrates use of a recently developed SAS macro, GLIMMIX, for implementing an analysis suggested by Wolfinger and O'Connell (1993) in modeling failure count data with random as well as fixed factor effects. Interest in this software tool arose from consideration of modernizing the Failure Rate Analysis Code (FRAC), developed at Los Alamos National Laboratory in the early 1980's by Martz, Beckman and McInteer (1982). FRAC is a FORTRAN program developed to analyze Poisson distributed failure count data as a log-linear model, possibly with random as well as fixed effects. These statistical modeling assumptions are a special case of generalized linear mixed models, identified as GLMM in the current statistics literature. In the nearly 15 years since FRAC was developed, there have been considerable advances in computing capability, statistical methodology and available statistical software tools allowing worthwhile consideration of the tasks of modernizing FRAC. In this paper, the approaches to GLMM estimation implemented in GLIMMIX and in FRAC are described and a comparison of results for the two approaches is made with data on catastrophic time-dependent pump failures from a report by Martz and Whiteman (1984). Additionally, statistical and graphical model diagnostics are suggested and illustrated with the GLIMMIX analysis results

  19. Color Parameters of the Chromascop Shade Guide

    Directory of Open Access Journals (Sweden)

    William J. O'Brien


    Full Text Available The purposes of this study are: (1 determine the color of the twenty shades in the Ivoclar’s Chromascop shade guide, (2 determine the color representation of the shade guide described as coverage error (CE, and (3 compare this shade guide with the Vita Classical and Bioform shade guides. The spectral data was collected using Beckman model DU reflectance spectrophotometer equipped with an integrating sphere. Commission International de l’Eclairage (CIE chromaticity coordinates were calculated using CIE illuminant C and 1931 observer data, then converted to CIE L*a*b* and Munsell notation. Each shade was spectrophotometrically compared to the published colors of 335 human teeth. The minimum CIE L*a*b* color difference was calculated for each tooth and the average of these color differences was defined as the CE. The measured colors of the Chromascop guide had a CIE L* range of 79.67 to 65.61, an a* range of -0.71 to 3.85, and a b* range of 14.58 to 27.69. The average CE of the Chromascop shade guide was 3.38. The Chromascop shade guide has similar colors and a CE compared with the Bioform and Vita Classical shade guides, but with some shades of higher red and yellow components.

  20. Cytotoxic effect of Erythroxylum suberosum combined with radiotherapy in head and neck cancer cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Macedo, Taysa B.C.; Torres, Hianne M.; Yamamoto-Silva, Fernanda Paula; Silva, Maria Alves G. [Universidade Federal de Goias (UFG), Goiania, GO (Brazil). Escola de Odontologia; Elias, Silvia T.; Silveira, Damaris; Magalhaes, Perola O.; Lofrano-Porto, Adriana; Guerra, Eliete N.S., E-mail: [Universidade de Brasilia (UnB), Brasilia, DF (Brazil). Faculdade de Ciencias da Saude


    The mouth and oropharynx cancer is the 6{sup th} most common type of cancer in the world. The treatment may involve surgery, chemotherapy and radiotherapy. More than 50% of drugs against cancer were isolated from natural sources, such as Catharanthus roseus and epipodophyllotoxin, isolated from Podophyllum. The biggest challenge is to maximize the control of the disease, while minimizing morbidity and toxicity to the surrounding normal tissues. The Erythroxylum suberosum is a common plant in the Brazilian Cerrado biome and is popularly known as 'cabelo-de-negro'. The objective of this study was to evaluate the cytotoxic activity of Erythroxylum suberosum plant extracts of the Brazilian Cerrado biome associated with radiotherapy in human cell lines of oral and hypopharynx carcinomas. Cells were treated with aqueous, ethanolic and hexanic extracts of Erythroxylum suberosum and irradiated at 4 Gy, 6 Gy and 8 Gy. Cytotoxicity was evaluated by MTT assay and the absorbance was measured at 570 nm in a Beckman Counter reader. Cisplatin, standard chemotherapy, was used as positive control. The use of Erythroxylum suberosum extracts showed a possible radiosensitizing effect in vitro for head and neck cancer. The cytotoxicity effect in the cell lines was not selective and it is very similar to the effect of standard chemotherapy. The aqueous extract of Erythroxylum suberosum, combined with radiotherapy was the most cytotoxic extract to oral and hypopharynx carcinomas. (author)

  1. Biomedical optics centers: forty years of multidisciplinary clinical translation for improving human health (United States)

    Tromberg, Bruce J.; Anderson, R. Rox; Birngruber, Reginald; Brinkmann, Ralf; Berns, Michael W.; Parrish, John A.; Apiou-Sbirlea, Gabriela


    Despite widespread government and public interest, there are significant barriers to translating basic science discoveries into clinical practice. Biophotonics and biomedical optics technologies can be used to overcome many of these hurdles, due, in part, to offering new portable, bedside, and accessible devices. The current JBO special issue highlights promising activities and examples of translational biophotonics from leading laboratories around the world. We identify common essential features of successful clinical translation by examining the origins and activities of three major international academic affiliated centers with beginnings traceable to the mid-late 1970s: The Wellman Center for Photomedicine (Mass General Hospital, USA), the Beckman Laser Institute and Medical Clinic (University of California, Irvine, USA), and the Medical Laser Center Lübeck at the University of Lübeck, Germany. Major factors driving the success of these programs include visionary founders and leadership, multidisciplinary research and training activities in light-based therapies and diagnostics, diverse funding portfolios, and a thriving entrepreneurial culture that tolerates risk. We provide a brief review of how these three programs emerged and highlight critical phases and lessons learned. Based on these observations, we identify pathways for encouraging the growth and formation of similar programs in order to more rapidly and effectively expand the impact of biophotonics and biomedical optics on human health.

  2. Cytotoxic effect of Erythroxylum suberosum combined with radiotherapy in head and neck cancer cell lines

    International Nuclear Information System (INIS)

    Macedo, Taysa B.C.; Torres, Hianne M.; Yamamoto-Silva, Fernanda Paula; Silva, Maria Alves G.; Elias, Silvia T.; Silveira, Damaris; Magalhaes, Perola O.; Lofrano-Porto, Adriana; Guerra, Eliete N.S.


    The mouth and oropharynx cancer is the 6 th most common type of cancer in the world. The treatment may involve surgery, chemotherapy and radiotherapy. More than 50% of drugs against cancer were isolated from natural sources, such as Catharanthus roseus and epipodophyllotoxin, isolated from Podophyllum. The biggest challenge is to maximize the control of the disease, while minimizing morbidity and toxicity to the surrounding normal tissues. The Erythroxylum suberosum is a common plant in the Brazilian Cerrado biome and is popularly known as 'cabelo-de-negro'. The objective of this study was to evaluate the cytotoxic activity of Erythroxylum suberosum plant extracts of the Brazilian Cerrado biome associated with radiotherapy in human cell lines of oral and hypopharynx carcinomas. Cells were treated with aqueous, ethanolic and hexanic extracts of Erythroxylum suberosum and irradiated at 4 Gy, 6 Gy and 8 Gy. Cytotoxicity was evaluated by MTT assay and the absorbance was measured at 570 nm in a Beckman Counter reader. Cisplatin, standard chemotherapy, was used as positive control. The use of Erythroxylum suberosum extracts showed a possible radiosensitizing effect in vitro for head and neck cancer. The cytotoxicity effect in the cell lines was not selective and it is very similar to the effect of standard chemotherapy. The aqueous extract of Erythroxylum suberosum, combined with radiotherapy was the most cytotoxic extract to oral and hypopharynx carcinomas. (author)

  3. Semiautomated system for the production and analysis of sucrose density gradients

    International Nuclear Information System (INIS)

    Lange, C.S.; Liberman, D.F.


    A semiautomated system in DNA damage studies permitting considerable accuracy, speed, and reproducibility in the making and fractionation of sucrose density gradients is described. The system consists of a modified Beckman gradient forming device that makes six gradients simultaneously and delivers them into six 12.5 ml polyallomer centrifuge tubes in such a manner that new material is continuously added to the meniscus of the gradient. The gradients are fractionated three at a time and up to 100 fractions per gradient can be collected automatically directly into scintillation vials with a choice of drop counting or time mode with rinse and automatic addition of scintillation fluid to each vial. The system can process up to six gradients per hour but centrifugation time is usually the limiting factor. With neutral sucrose gradients, sharp, reproducible, monodisperse peaks containing up to 100 percent of the gradient radioactivity are usually obtained but a smaller monodisperse peak containing as little as 3.5 percent of the gradient radioactivity can be detected under conditions where some pairs of molecules might tangle or dimerize. The resolution and reproducibility of this system when used with neutral sucrose gradients is at least the equal if not superior to that commonly claimed for alkaline sucrose gradients. (U.S.)

  4. Experimental studies on SO/sub 2/ injuries in higher plants. Part 4

    Energy Technology Data Exchange (ETDEWEB)

    Tanaka, H; Takanashi, T; Yatazawa, M


    The effect of sulfur dioxide on aminoacid metabolism was examined. Wheat seedlings one month old (20 cm) were exposed to 0.5 ppm SO/sub 2/ at 22/sup 0/C, and 40% humidity for four days, only during the day. A Beckman 1200 aminoacid analyzer revealed only slight effects, but it was conjectured that the plant had some separate amino acid in its body prior to the test, and that the effect of SO/sub 2/ was relatively low. A similar wheat seedling was placed in a closed glass container and was exposed to 300 ppm carbon dioxide (14) and 100 ppm SO/sub 2/ for 30 min under 4000 lux. The first paper chromatography of 80% ethanol solubles from the leaves revealed that Ala. value had increased but sir. had decreased in half. The second paper chromatography revealed that in both test and control plants, four kinds of material in amino acid had taken in C(14), and two of the four were Ser. Ala. The total C(14) taken in by the test material under SO/sub 2/ exposure showed a noted decrease. The experiment proved that exposure of a plant to a high concentration of SO/sub 2/ produced hydroxysulfonate in metabolism and impaired its glycol acid function.

  5. Long-Term Stability of Photovoltaic Hybrid Perovskites achieved by Graphene Passivation via a Water- and Polymer-Free Graphene Transfer Method (United States)

    Tseng, W.-S.; Jao, M.-H.; Hsu, C.-C.; Wu, C.-I.; Yeh, N.-C.

    Organic-inorganic hybrid perovskites such as CH3NH3PbX3 (X = I, Br) have been intensively studied in recent years because of their rapidly improving photovoltaic power conversion efficiency. However, severe instability of these materials in ambient environment has been a primary challenge for practical applications. To address this issue, we employ high-quality PECVD-grown graphene to passivate the hybrid perovskites. In contrast to existing processes for transferring graphene from the growth substrates to other surfaces that involve either polymer or water, which are incompatible with photovoltaic applications of these water-sensitive hybrid perovskites, we report here a new water- and polymer-free graphene transferring method. Studies of the Raman, x-ray and ultraviolet photoemission spectroscopy (XPS and UPS) demonstrated excellent quality of monolayer PECVD-grown graphene samples after their transfer onto different substrates with the water- and polymer-free processing method. In particular, graphene was successfully transferred onto the surface of CH3NH3PbI3 thin films with sample quality intact. Moreover, XPS and UPS studies indicated that even after 3 months, the fully graphene-covered perovskite films remained spectroscopically invariant, which was in sharp contrast to the drastic changes, after merely one week, in both the XPS and UPS of a control CH3NH3PbI3 sample without graphene protection. Beckman Inst. in Caltech. Dragon Gate Program in Taiwan.

  6. Age-independent anti-Müllerian hormone (AMH) standard deviation scores to estimate ovarian function. (United States)

    Helden, Josef van; Weiskirchen, Ralf


    To determine single year age-specific anti-Müllerian hormone (AMH) standard deviation scores (SDS) for women associated to normal ovarian function and different ovarian disorders resulting in sub- or infertility. Determination of particular year median and mean AMH values with standard deviations (SD), calculation of age-independent cut off SDS for the discrimination between normal ovarian function and ovarian disorders. Single-year-specific median, mean, and SD values have been evaluated for the Beckman Access AMH immunoassay. While the decrease of both median and mean AMH values is strongly correlated with increasing age, calculated SDS values have been shown to be age independent with the differentiation between normal ovarian function measured as occurred ovulation with sufficient luteal activity compared with hyperandrogenemic cycle disorders or anovulation associated with high AMH values and reduced ovarian activity or insufficiency associated with low AMH, respectively. These results will be helpful for the treatment of patients and the ventilation of the different reproductive options. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. Random distribution of nucleoli in metabolic cells

    Energy Technology Data Exchange (ETDEWEB)

    Beckman, R.J.; Waterman, M.S.


    Hasofer (1974) has studied a probabilistic model for the fusion of nucleoli in metabolic cells. The nucleoli are uniformly distributed at points in the nucleus, assumed to be a sphere. The nucleoli grow from a point to a maximum size during interphase, and fusion is said to occur if the nucleoli touch. For this model, Hasofer calculated the probability of fusion and found it much smaller than experimental data would indicate. Experimental data of this type is taken by use of a microscope where a two-dimensional view or projection of the three-dimensional cell is obtained. Hasofer implicitly assumes that actual fusion can be distinguished from the case where the two nucleoli do not touch but their two-dimensional projections overlap. It is assumed, in this letter, that these two cases cannot be distinguished. The probability obtained by Beckman and Waterman is larger than Hasofer's and a much better fit to the experimental data is obtained. Even if true fusion can be unfailingly distinguished from overlap of the two-dimensional projections, it is hoped that these calculations will allow someone to propose the correct (non-uniform) model. It is concluded, for the assumptions used, that there is not sufficient evidence to reject the hypothesis of uniform distribution of the nucleoli.

  8. A new method of quench monitoring in liquid scintillation counting

    International Nuclear Information System (INIS)

    Horrocks, D.L.


    The quench level of different liquid scintillation counting samples is measured by comparing the responses (pulse heights) produced by the same energy electrons in each sample. The electrons utilized in the measurements are those of the maximum energy (Esub(max)) which are produced by the single Compton scattering process for the same energy gamma-rays in each sample. The Esub(max) response produced in any sample is related to the Esub(max) response produced in an unquenched, sealed standard. The difference in response on a logarithm response scale is defined as the ''H Number''. The H number is related to the counting efficiency of the desired radionuclide by measurement of a set of standards of known amounts of the radionuclide and different amounts of quench (standard quench curve). The concept of the H number has been shown to be theoretically valid. Based upon this proof, the features of the H number concept as embodied in the Beckman LS-8000 Series Liquid Scintillation Systems have been demonstrated. It has been shown that one H number is unique; it provides a method of instrument calibration and wide dynamic quench range measurements. Further, it has been demonstrated that the H number concept provides a universal quench parameter. Counting efficiency vs. H number plots are repeatable within the statistical limits of +-1% counting efficiency. By the use of the H number concept a very accurate method of automatic quench compensation (A.Q.C.) is possible. (T.G.)

  9. CUSUM-Logistic Regression analysis for the rapid detection of errors in clinical laboratory test results. (United States)

    Sampson, Maureen L; Gounden, Verena; van Deventer, Hendrik E; Remaley, Alan T


    The main drawback of the periodic analysis of quality control (QC) material is that test performance is not monitored in time periods between QC analyses, potentially leading to the reporting of faulty test results. The objective of this study was to develop a patient based QC procedure for the more timely detection of test errors. Results from a Chem-14 panel measured on the Beckman LX20 analyzer were used to develop the model. Each test result was predicted from the other 13 members of the panel by multiple regression, which resulted in correlation coefficients between the predicted and measured result of >0.7 for 8 of the 14 tests. A logistic regression model, which utilized the measured test result, the predicted test result, the day of the week and time of day, was then developed for predicting test errors. The output of the logistic regression was tallied by a daily CUSUM approach and used to predict test errors, with a fixed specificity of 90%. The mean average run length (ARL) before error detection by CUSUM-Logistic Regression (CSLR) was 20 with a mean sensitivity of 97%, which was considerably shorter than the mean ARL of 53 (sensitivity 87.5%) for a simple prediction model that only used the measured result for error detection. A CUSUM-Logistic Regression analysis of patient laboratory data can be an effective approach for the rapid and sensitive detection of clinical laboratory errors. Published by Elsevier Inc.

  10. Preparation and infrared spectra of differently deuterated tetramethyl-derivatives of the IV. main group

    International Nuclear Information System (INIS)

    Biedermann, S.


    19 different deuterated tetramethyl derivates of the type (CH 3 )sub(4-n)M(CH 3 )sub(n) of C, Si, Ge, Sn and Pb were prepared. Gas cuvettes with polyethylene, NaCl and KBr windows were used to absorb the infra-red spectra, the IR equipment Beckman IR 11 and IR 12 were used. The infra-red spectra of the above mentioned compounds were indicated from 33 to 4,000 cm -1 , the ground, upper and combination vibrations assigned, the PR separations of the partly well resolved rotation-vibration outlines determined and were compared with the calculated PR separations. The revision of the correlations performed by Graham for γsub(s)CH 3 and γsub(as)CH 3 in the race of vibration F 2 with Sn(CH 3 ) 4 and Pb(CH 3 ) 4 and the proposed one with C(CH 3 ) 4 , Si(CH 3 ) 4 and Ge(CH 3 ) 4 could be disproved by the new experimental results. (FW) [de

  11. Solid scintillator 'Ready Cap' for measurement with a liquid scintillation counter

    International Nuclear Information System (INIS)

    Ijiri, Kenichi; Endo, Masashi; Nogawa, Norio; Tsuda, Shoko; Nakamura, Aiko; Morikawa, Naotake; Osaki, Susumu.


    'Ready Cap', a small plastic container coated with solid scintillator has recently been introduced (Beckman Instruments, Inc.). Pulse height spectra and counting efficiencies obtained with a liquid scintillator and Ready Cap using a liquid scintillation counter were compared for 15 different radionuclides. For radionuclides emitting low-energy β-rays or characteristic X-rays, the spectra for Ready Cap shifted toward the higher energy side compared with the spectra for the liquid scintillator. This tendency was reversed for the nuclides emitting higher-energy β-radiations ( 36 Cl and 32 P). Generally, counting efficiencies both in Ready Cap and in liquid scintillator increased with increase in the energy of β- or X-rays. For some nuclides, Ready Cap gave higher counting efficiencies and for others it gave lower values than in the liquid scintillator. However, the differences were not large within each nuclide. The use of Ready Cap is recommended for measurements of radionuclides when liquid scintillation cocktails have no means of waste disposal under the present Japanese radioisotope regulation. (author)

  12. Validation of the HTO-18 method for determination of CO2 production of lizards (genus Sceloporus)

    International Nuclear Information System (INIS)

    Congdon, J.D.; King, W.W.; Nagy, K.A.


    The accuracy of doubly-labeled water measurements of CO 2 production in lizards of the genus Sceloporus was assessed by comparison of CO 2 production rates determined simultaneously by labeled water and gas chromatography. Five lizards were weighed and given intraperitoneal injections of 55 μl of water containing 10 microcuries of tritium as HTO and 50 atom % oxygen-18 as H 2 18 O. Initial blood samples were taken from the infraorbital sinus ten hours later, and the lizards were placed in sealed metabolism chambers kept at 28 C. After 179 h the lizards were weighed and blood samples taken. Blood samples were microdistilled, assayed for tritium activity and for oxygen-18 content. Isotope measurements were used to calculate rates of CO 2 production. Gas samples were withdrawn from each chamber after 18, 63, 109, and 179 h and measured against 0.5 and 1.0% CO 2 standards with a Beckman GC-55 gas chromatograph fitted with silica gel 42-60 mesh column. These results were used to calculate rates of CO 2 production. Results supported the conclusion that the doubly-labeled water method accurately measured rates of CO 2 production in Sceloporus lizards, and could therefore be a valuable technique in field studies of lizard energetics

  13. Goeckerman's therapy for psoriasis with special reference to serum pentraxin 3 level

    Energy Technology Data Exchange (ETDEWEB)

    Ctirad, A.; Lenka, B.; David, P.; Zdenek, F.; Kveta, H.; Karel, E.; Jan, K. [Charles University Prague, Hradec Kralove (Czech Republic). University Hospital


    Goeckerman's therapy (GT) of psoriasis is based on daily application of pharmacy grade coal tar on affected skin with subsequent exposure to UV light. Pentraxin 3 (PTX3) is a newly identified acute phase reactant with non redundant functions in innate immunity. PTX3 has been shown to be a reliable prognostic marker in patients with various inflammatory disorders including rheumatoid arthritis, vasculitis, and psoriasis. The aim of this study was to evaluate the influence of Goeckerman's therapy of psoriasis on levels of two pentraxins: long pentraxin PTX3 and C reactive protein in 49 patients with chronic plaque psoriasis. CRP was assessed by immunonephelometry on IMMAGE 800 (Beckman, USA). PTX3 was detected using sandwich ELISA detection set (Alexis Biochemicals, Switzerland). The serum levels of both parameters (expressed as average {+-} 1 SD) were significantly diminished after GT. The level of PTX3 dropped from 1.92 {+-} 0.72 ng/ml before GT to 1.66 {+-} 0.58 ng/ml after GT (P = 0.0396) and the level of CRP fell from 4.64 {+-} 3.93 mg/l to 1.66 {+-} 0.58 mg/l (P {lt} 0.0001). Comparing to healthy controls, the serum levels of both parameters before GT were significantly higher than those found in healthy blood donors and remained significantly increased after GT. Increased serum concentrations of pentraxin 3 and CRP are alleviated by GT in patients with psoriasis.

  14. Smoking cessation: an application of theory of planned behavior to understanding progress through stages of change. (United States)

    Bledsoe, Linda K


    The purpose of this research was to investigate variables relevant to smoking cessation early in the process of change through an application of the Theory of Planned Behavior [Ajzen, I. (1985). From intentions to actions: A theory of planned behavior. In J. Kuhl and J. Beckman (Eds). Action-control: From cognition to behavior (pp.11-39). Heidelberg: Springer.] to the temporal structure provided by the Transtheoretical Model. Study 1 was a preliminary elicitation study (n=68) conducted to ground the concepts used in the model testing in Study 2 [Ajzen, I., Fishbein, M. (1980). Understanding attitudes and predicting social behavior, Englewood Cliffs, NJ: Prentice-Hall.]. Study 2 tested the proposed model fit with data from a sample of 230 adult smokers. Structural equation modeling did not support the Theory of Planned Behavior as a model of motivation for progress through the stages of change and highlighted measurement issues with perceived behavioral control. A modified model using the Theory of Reasoned Action provided a good fit to the data, accounting for approximately 64% of the variance in intention to quit smoking and stage of change. This research addresses the need for a more complete theoretical rationale for progress through stages of change.

  15. Amino acid composition of some Mexican foods. (United States)

    Morales de León, Josefina; Camacho, M Elena; Bourges, Héctor


    Knowledge of the amino acid composition of foods is essential to calculate their chemical score, which is used to predict protein quality of foods and diets. Though amino acid composition of many foods is reasonably well established, better knowledge is needed on native foods consumed in different regions and countries. This paper presents the amino acid composition of different presentations of raw and processed foods produced and consumed in Mexico. The amino acid composition was determined using Beckman amino acid analyzers (models 116 and 6300). Tryptophan was determined using the Spies and Chambers method. Of the different foods analyzed, some comments are made on native or basic foods in Mexico: Spirulin, where lysine is the limiting amino acid, with a chemical score of 67%, is a good source of tryptophan (1.16g/16 gN); amaranth contains high levels of sulphur amino acids (4.09 to 5.34 g/16gN), with a protein content of 15 g/100g; and pulque, a Pre-Hispanic beverage that contains high levels of tryptophan (2.58 g/16 gN) and sulphur amino acids (2.72 g/16 gN). Finally, insects are good sources of sulphur amino acids and lysine.

  16. Roger Hayward and the Invention of the Two-Mirror Schmidt (United States)

    Bell, T. E.


    Roger Hayward (1899-1979), now virtually unknown, was a multitalented architect, scientific illustrator, and optical inventor. Remembered primarily for illustrating Scientific American magazine's Amateur Scientist column between 1949 and 1974, he also illustrated more than a dozen textbooks in optics, physics, geology, oceanography, and chemistry, several of which became classics in their fields. He designed façades with astronomical themes for major buildings in Los Angeles, California, and sculpted mammoth, realistic models of the moon for Griffith Observatory, Adler Planetarium, and Disneyland. Throughout his life, he recreationally painted watercolors and oils that at least one critic likened to the work of John Singer Sargent. Hayward is least known as an optical designer, yet he made significant contributions to the DU spectrophotometer that established the multimillion-dollar company Beckman Instruments. During the pre-radar days of World War II at Mount Wilson Observatory, Hayward invented a classified Cassegrain version of the Schmidt telescope especially adapted for nighttime infrared aerial photography, plus extraordinarily simple machines that allowed inexperienced soldiers to grind, polish, and test accurate aspheric Schmidt correcting plates at speeds compatible with mass production - and later received U.S. patents for them all. This paper, drawn in part from unpublished letters between Hayward and Albert G. Ingalls, will feature little-known images of Hayward's work.

  17. [Determination of ciprofloxacin in human serum and urine by reversed-phase HPLC]. (United States)

    Qin, Y; Liang, D


    A sensitive and rapid method for the determination of ciprofloxacin using enoxacin as the internal standard was reported. High-performance liquid chromatograph model 344 (Beckman, USA) with a variable wavelength UV detector and reversed-phase Ultrasphere-ODS column (5 microns, 250 x 4.6 mm) was used. Serum or urine sample preparation involved addition phosphate buffer (pH 7.5) and aqueous solution of sodium lauryl sulfate, followed by chloroform extraction. The organic layer was removed and evaporated to dryness under an air stream in a 37 degrees C water bath. The residue was dissolved in 50 microliters mobile phase and 20 microliters injected. The mobile phase of 0.02 mol/L acetate buffer (pH 3.0) -acetonitril-dimethylformamide-10% aqueous solution of tetrabutyl ammonium hydroxide (88:6.5:5:0.5) was pumped at 0.9 ml/min through the column. The detector operated at 0.01 aufs and the wavelength was set at 276 nm. The retention times for ciprofloxacin and enoxacin were 7.31 min and 5.59 min, respectively. In serum, standard curve was linear in the concentration range of 0.75 to 24 mumol/L, the detective limit was 0.2 mumol/L, extraction recovery was 69-74%, within-day CV was less than 5%, and inter-day CV was less than 6%.

  18. Harmonisation of seven common enzyme results through EQA. (United States)

    Weykamp, Cas; Franck, Paul; Gunnewiek, Jacqueline Klein; de Jonge, Robert; Kuypers, Aldy; van Loon, Douwe; Steigstra, Herman; Cobbaert, Christa


    Equivalent results between different laboratories enable optimal patient care and can be achieved with harmonisation. We report on EQA-initiated national harmonisation of seven enzymes using commutable samples. EQA samples were prepared from human serum spiked with human recombinant enzymes. Target values were assigned with the IFCC Reference Measurement Procedures. The same samples were included at four occasions in the EQA programmes of 2012 and 2013. Laboratories were encouraged to report IFCC traceable results. A parallel study was done to confirm commutability of the samples. Of the 223 participating laboratories, 95% reported IFCC traceable results, ranging from 98% (ASAT) to 87% (amylase). Users of Roche and Siemens (97%) more frequently reported in IFCC traceable results than users of Abbott (91%), Beckman (90%), and Olympus (87%). The success of harmonisation, expressed as the recovery of assigned values and the inter-laboratory CV was: ALAT (recovery 100%; inter-lab CV 4%), ASAT (102%; 4%), LD (98%; 3%), CK (101%; 5%), GGT (98%; 4%), AP (96%; 6%), amylase (99%; 4%). There were no significant differences between the manufacturers. Commutability was demonstrated in the parallel study. Equal results in the same sample in the 2012 and 2013 EQA programmes demonstrated stability of the samples. The EQA-initiated national harmonisation of seven enzymes, using stable, commutable human serum samples, spiked with human recombinant enzymes, and targeted with the IFCC Reference Measurement Procedures, was successful in terms of implementation of IFCC traceable results (95%), recovery of the target (99%), and inter-laboratory CV (4%).

  19. Low density lipoprotein for oxidation and metabolic studies. Isolation from small volumes of plasma using a tabletop ultracentrifuge. (United States)

    Himber, J; Bühler, E; Moll, D; Moser, U K


    A rapid method is described for the isolation of small volumes of plasma low density lipoprotein (LDL) free of plasma protein contaminants using the TL-100 Tabletop Ultracentrifuge (Beckman). The isolation of LDL was achieved by a 25 min discontinuous gradient density centrifugation between the density range of 1.006 and 1.21 g/ml, recovery of LDL by tube slicing followed by a 90 min flotation step (d = 1.12 g/ml). The purity of LDL and apolipoprotein B100 (apo B100) were monitored by agarose electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), radial immunodiffusion and micropreparative fast protein liquid chromatography (FPLC). The ability of LDL oxidation was assessed by following absorbance at 234 nm after addition of copper ions. The functional integrity of the isolated LDL was checked by clearance kinetics after injection of [125I]-labelled LDL in estrogen-treated rats. The additional purification step led to LDL fractions free of protein contamination and left apo B100, alpha-tocopherol and beta-carotene intact. The LDL prepared in this way was free of albumin, as evident from analytic tests and from its enhanced oxidative modification by copper ions. Used for analytical purposes, this method allows LDL preparations from plasma volumes up to 570 microliters. This method is also convenient for metabolic studies in small animals, especially those relating to the determination of kinetic parameters of LDL in which LDL-apo B100 has to be specifically radiolabelled.

  20. Bone Ablation at 2.94 mm Using the Free-Electron Laser and Er:YAG Laser (United States)

    Ivanov, Borislav; Hakimian, Arman; Peavy, G. M.; Haglund, Richard


    Bone Ablation at 2.94 microns Using the Free-Electron Laser and Er:YAG Laser in Perfluorocarbon Compounds B. Ivanov^1, A. M. Hakimian^1, G. M. Peavy^2, R. F. Haglund, Jr.1 1Department of Physics and Astronomy, W. M. Keck Foundation Free-Electron Laser Center, Vanderbilt University, Nashville, TN 37235 2Beckman Laser Institute and Medical Clinic, College of Medicine, University of California, Irvine, CA 92612 We report studies on the efficiency of mid-IR laser ablation of cow cortical bone using the Vanderbilt free-electron laser (FEL), when irrigating the ablation zone with an inert and biocompatible perfluorocarbon compounds (PFC). At 2.94 microns, the bone matrix (mainly by water) absorbs the radiation while the PFCs transmit this wavelength, dissipate heat and acoustical stress, and prevent carbonization of the bone sample. The ablation rate, as a function of laser fluence, scanning speed and the type of PFC, was investigated. The laser fluence was estimated to be 5 J/cm^2 - 100 J/cm^2 with a laser focal spot diameter of 160 microns 500 microns and a scanning speed of 40 microns/s 2960 microns/s. The ablation rate was estimated from scanning electron microscopy to be 0.5 mm/s 2.4 mm/s. Comparisons of ablation rates with the FEL and a Er:YAG laser at 2.94 microns are being evaluated.

  1. Analytical and clinical performance of the new Fujirebio 25-OH vitamin D assay, a comparison with liquid chromatography-tandem mass spectrometry (LC-MS/MS) and three other automated assays. (United States)

    Saleh, Lanja; Mueller, Daniel; von Eckardstein, Arnold


    We evaluated the analytical and clinical performance of the new Lumipulse® G 25-OH vitamin D assay from Fujirebio, and compared it to a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and three other commercial automated assays. Total 25 hydroxy vitamin D (25(OH)D) levels were measured in 100 selected serum samples from our routine analysis with Fujirebio 25(OH)D assay. The results were compared with those obtained with LC-MS/MS and three other automated 25(OH)D assays (Abbott, Beckman, and Roche). The accuracy of each assay tested was evaluated against a Labquality reference serum panel for 25(OH)D (Ref!25OHD; University of Ghent). Intra- and inter-day imprecision of the Fujirebio 25(OH)D assay was Lumipulse G 25-OH vitamin D assay from Fujirebio demonstrated a good correlation with LC-MS/MS and some immunoassays. The performance of the assay is well-suited for routine 25(OH)D measurement in clinical serum samples. A correction for the observed negative bias vs. LC-MS/MS could be considered.

  2. Emerging role of brain metastases in the prognosis of breast cancer patients

    Directory of Open Access Journals (Sweden)

    Hambrecht A


    Full Text Available Amanda Hambrecht1,2, Rahul Jandial2, Josh Neman21Department of Biology, University of Southern California; 2Department of Neurosurgery, Beckman Research Institute, City of Hope National Cancer Center, CA, USAAbstract: Cancer starts with one rogue cell. Through mutations and genomic alterations, the cell acquires specific and stem cell-like characteristics necessary for invasion of a distant organ and ultimately metastasis. Metastatic brain cancer is a particularly formidable disease because of its poor prognosis and the highly resistant nature of the tumor to chemotherapy. Although several types of primary tumors have a tendency to metastasize to the brain, the incidence of brain metastases has increased dramatically in some subsets of breast cancer patients. Several conventional treatments are available, but success is limited and often short-lived. Given that no standard treatment options exist, there is a significant need to investigate the biology of these clinically recalcitrant tumors. Keywords: metastasis, breast cancer, blood-brain barrier, epithelial-mesenchymal transition, mesenchymal-epithelial transition

  3. Traumatic floating clavicle: a case report and literature review. (United States)

    Gouse, Mohamad; Jacob, Korula Mani; Poonnoose, Pradeep Mathew


    Bipolar fracture dislocations of the clavicle are rare injuries, usually the result of high-energy direct trauma. Since the original description by Porral in 1831, only a handful of individual case reports and case series by Beckman and Sanders have been reported in the literature. Management of these injuries has remained controversial ranging from nonoperative to aggressive surgery. We report on the case of a young army cadet who had a fracture of the lateral end of the clavicle, with an anterior dislocation of the sternoclavicular joint. Despite being planned for surgery, at the patients request, it was decided to manage the lesion conservatively with graded physiotherapy. At one-year follow-up, he had full pain-free, functional range of movement of the shoulder. This young high demand patient had a good outcome with conservative management, despite going against the current trend towards surgical treatment. We present this case with a review of the literature, highlighting the various management options for this rare lesion.

  4. Interference of ascorbic acid with chemical analytes. (United States)

    Meng, Qing H; Irwin, William C; Fesser, Jennifer; Massey, K Lorne


    Ascorbic acid can interfere with methodologies involving redox reactions, while comprehensive studies on main chemistry analysers have not been reported. We therefore attempted to determine the interference of ascorbic acid with analytes on the Beckman Synchron LX20. Various concentrations of ascorbic acid were added to serum, and the serum analytes were measured on the LX20. With a serum ascorbic acid concentration of 12.0 mmol/L, the values for sodium, potassium, calcium and creatinine increased by 43%, 58%, 103% and 26%, respectively (Pascorbic acid concentration of 12.0 mmol/L, the values for chloride, total bilirubin and uric acid decreased by 33%, 62% and 83%, respectively (Pcholesterol, triglyceride, ammonia and lactate. There was no definite influence of ascorbic acid on analytical values for total CO(2), urea, glucose, phosphate, total protein, albumin, amylase, creatine kinase, creatine kinase-MB, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total iron, unbound iron-binding capacity or magnesium. Ascorbic acid causes a false increase in sodium, potassium, calcium and creatinine results and a false decrease in chloride, total bilirubin, uric acid, total cholesterol, triglyceride, ammonia and lactate results.

  5. “Fiz péssima escolha” na tomada de decisão

    Directory of Open Access Journals (Sweden)

    Antonio Mendes Silva Filho


    Full Text Available Há uma frase (do Steve Beckman que diz “você faz suas escolhas e suas escolhas fazem você.” Todo indivíduo é dotado de habilidades que formam suas competências e lhe permite decisões. Há dias atrás, uma frase de minhas leituras me chamou a atenção, “fiz péssima escolha”. Independente do contexto ao qual ela estava inserida, o que aqui se destaca e desperta atenção é: o que leva uma pessoa a dizer “fiz péssima escolha”? Será errar ou medo de errar algo ruim? Será o descarte a solução dessa “péssima escolha”? Ou será a percepção do resultado da escolha equivocada? Este artigo visa explorar questões que podem motivar um personagem ou pessoa a se expressar dessa maneira, buscando explorar traços da natureza humana.

  6. Radiation damage to mouse testis cells from [/sup 99m/Tc] pertechnetate

    International Nuclear Information System (INIS)

    Mian, T.A.; Suzuki, N.; Glenn, H.J.; Haynie, T.P.; Meistrich, M.L.


    The radiation dose and the biologic damage to mouse testis from intravenously administered [/sup 99m/Tc] pertechnetate were studied. The dose was measured for penetrating radiations from /sup 99m/Tc, using calibrated thermoluminescent dosimeters and calculations from the uptake of the nuclide in the testis, and was found to be 4.9 rads per mCi of 99 Tc. The biologic damage was measured by the decrease in the number of sperm heads in the testis, counted both by hemacytometer and by Coulter counter. In preliminary experiments using external gamma radiation from 137 Cs, the number of sperm heads reached a minimum 29 days after irradiation. Twenty-nine days after injection of 5.8 mCi of /sup 99m/Tc, which gives 28 rads to the testis, the number of sperm heads decreased to 70% of control. The biologic effect corresponds to that seen after 40 rads of gamma radiation from 137 Cs. The damage to mouse testis cells from internally administered /sup 99m/Tc as measured in an in vivo system appears to be at least as significant as that from external gamma irradiation, if not more so

  7. Analysis of the Budding Yeast Cell Cycle by Flow Cytometry. (United States)

    Rosebrock, Adam P


    DNA synthesis is one of the landmark events in the cell cycle: G 1 cells have one copy of the genome, S phase cells are actively engaged in DNA synthesis, and G 2 cells have twice as much nuclear DNA as G 1 cells. Cellular DNA content can be measured by staining with a fluorescent dye followed by a flow-cytometric readout. This method provides a quantitative measurement of cell cycle position on a cell-by-cell basis at high speed. Using flow cytometry, tens of thousands of single-cell measurements can be generated in a few seconds. This protocol details staining of cells of the budding yeast Saccharomyces cerevisiae for flow cytometry using Sytox Green dye in a method that can be scaled widely-from one sample to many thousands and operating on inputs ranging from 1 million to more than 100 million cells. Flow cytometry is preferred over light microscopy or Coulter analyses for the analysis of the cell cycle as DNA content and cell cycle position are being directly measured. © 2017 Cold Spring Harbor Laboratory Press.

  8. Monitoring of Non-Ferrous Wear Debris in Hydraulic Oil by Detecting the Equivalent Resistance of Inductive Sensors

    Directory of Open Access Journals (Sweden)

    Lin Zeng


    Full Text Available Wear debris in hydraulic oil contains important information on the operation of equipment, which is important for condition monitoring and fault diagnosis in mechanical equipment. A micro inductive sensor based on the inductive coulter principle is presented in this work. It consists of a straight micro-channel and a 3-D solenoid coil wound on the micro-channel. Instead of detecting the inductance change of the inductive sensor, the equivalent resistance change of the inductive sensor is detected for non-ferrous particle (copper particle monitoring. The simulation results show that the resistance change rate caused by the presence of copper particles is greater than the inductance change rate. Copper particles with sizes ranging from 48 μm to 150 μm were used in the experiment, and the experimental results are in good agreement with the simulation results. By detecting the inductive change of the micro inductive sensor, the detection limit of the copper particles only reaches 70 μm. However, the detection limit can be improved to 48 μm by detecting the equivalent resistance of the inductive sensor. The equivalent resistance method was demonstrated to have a higher detection accuracy than conventional inductive detection methods for non-ferrous particle detection in hydraulic oil.

  9. Encapsulation of Platelet in Kefiran Polymer and Detection of Bioavailability of Immobilized Platelet in Probiotic Kefiran as A New Drug for Surface Bleeding

    Directory of Open Access Journals (Sweden)

    Anahita Jenab


    Full Text Available Background : Kefir contains lactic acid bacteria (Lactobacillus, Lactococcus, Leuconostoc, Acetobacter and Streptococcus and yeasts (Kluyveromyces, Torula, Candida, Saccharomyces .Kefiran is the polysaccharide produced by lactic acid bacteria in kefir.Methods : Kefiran was prepared from milk containing 0.5% fat and 10 grams kefir grains and was separated from kefir by ethanol (0.02 gram following entrapping the platelets to this polymer. Ligand of the platelet-polysaccharide was studied by FTIR.Results : FTIR results showed that the bands of C-O and C-O-C connections were formed and the polysaccharides had been attached to the receptors of the platelet glycoproteins (GP Ib,GPIIb / IIIa. Stability and encapsulation of the platelet and kefiran were assessed by Coulter Counter. Encapsulation of the platelets by polysaccharide at the beginning caused to reduce the number of platelets following by releasing of 50% of the platelets after 3 hours.Conclusion : The platelets were encapsulated in kefiran polymer and detected for bioavailability as new drug for surface bleeding. Also, kefiran has antimicrobial and antifungal properties. On the other hand, the existence of nisin in kefiran could be useful as an antibacterial lantibiotic. 

  10. Analytical scale purification of zirconia colloidal suspension using field programmed sedimentation field flow fractionation. (United States)

    Van-Quynh, Alexandra; Blanchart, Philippe; Battu, Serge; Clédat, Dominique; Cardot, Philippe


    Sedimentation field flow fractionation was used to obtain purified fractions from a polydispersed zirconia colloidal suspension in the potential purpose of optical material hybrid coating. The zirconia particle size ranged from 50/70 nm to 1000 nm. It exhibited a log-Gaussian particle size distribution (in mass or volume) and a 115% polydispersity index (P.I.). Time dependent eluted fractions of the original zirconia colloidal suspension were collected. The particle size distribution of each fraction was determined with scanning electron microscopy and Coulter sub-micron particle sizer (CSPS). These orthogonal techniques generated similar data. From fraction average elution times and granulometry measurements, it was shown that zirconia colloids are eluted according to the Brownian elution mode. The four collected fractions have a Gaussian like distribution and respective average size and polydispersity index of 153 nm (P.I. = 34.7%); 188 nm (P.I. = 27.9%); 228 nm (P.I. = 22.6%), and 276 nm (P.I. = 22.3%). These data demonstrate the strong size selectivity of SdFFF operated with programmed field of exponential profile for sorting particles in the sub-micron range. Using this technique, the analytical production of zirconia of given average size and reduced polydispersity is possible.

  11. Physical properties and structure of fine core-shell particles used as packing materials for chromatography Relationships between particle characteristics and column performance. (United States)

    Gritti, Fabrice; Leonardis, Irene; Abia, Jude; Guiochon, Georges


    The recent development of new brands of packing materials made of fine porous-shell particles, e.g., Halo and Kinetex, has brought great improvements in potential column efficiency, demanding considerable progress in the design of chromatographic instruments. Columns packed with Halo and Kinetex particles provide minimum values of their reduced plate heights of nearly 1.5 and 1.2, respectively. These packing materials have physical properties that set them apart from conventional porous particles. The kinetic performance of 4.6mm I.D. columns packed with these two new materials is analyzed based on the results of a series of nine independent and complementary experiments: low-temperature nitrogen adsorption (LTNA), scanning electron microscopy (SEM), inverse size-exclusion chromatography (ISEC), Coulter counter particle size distributions, pycnometry, height equivalent to a theoretical plate (HETP), peak parking method (PP), total pore blocking method (TPB), and local electrochemical detection across the column exit section (LED). The results of this work establish links between the physical properties of these superficially porous particles and the excellent kinetic performance of columns packed with them. It clarifies the fundamental origin of the difference in the chromatographic performances of the Halo and the Kinetex columns. Copyright 2010 Elsevier B.V. All rights reserved.

  12. How does the greater white-toothed shrew, Crocidura russula, responds to long-term heavy metal contamination? - A case study

    International Nuclear Information System (INIS)

    Marques, Carla Cristina; Sanchez-Chardi, Alejandro; Gabriel, Sofia Isabel; Nadal, Jacint; Viegas-Crespo, Ana Maria; Luz Mathias, Maria da


    Heavy metals accumulation in parallel with the evaluation of physiological and biochemical effects resulting from continued metal exposure were considered here using for the first time the great white-toothed shrew Crocidura russula as an in vivo model. Shrews were originated from an abandoned lead/zinc mining area and from a reference area, both in Alentejo, southern Portugal. Hepatic contents of nickel, copper, zinc, cadmium, mercury and lead were quantified by Inductively Coupled Plasma Mass Spectrometer (ICP-MS). Haematological parameters (white blood cells, red blood cells, haemoglobin and haematocrit) were obtained in a Coulter Counter Analyser and biochemical markers of the redox balance (glutathione S-transferase, glutathione peroxidase, and glutathione reductase) activities were measured spectrophotometrically using a Duo-50 spectrophotometer. Compared with control animals, significantly higher concentration of hepatic cadmium (9.29 vs. 1.18 μg/g dry weight) and nickel (1.56 vs. 0.343 μg/g dry weight) were detected in the shrews collected in the mining area. However, no significant changes were observed on haematological or enzymatic parameters in animals exposed to metal pollution. The obtained results show that shrews are good bioaccumulators of toxic heavy metals, but very tolerant to their effects, revealing an interesting long-term adaptation to polluted environments. In addition, this study provides reference values for haematological parameters and antioxidant enzymes levels in C. russula, which may be relevant for comparative purposes in further studies

  13. Premarital Screening of Beta Thalassemia Minor in north-east of Iran (United States)

    Hashemizadeh, H; Noori, R


    Background Beta thalassemia is a preventable disease. Iran has about 20,000Patients who are homozygote for β-thalassaemia and 3,750,000 carriers. The aim of this study was to determine the prevalence of beta thalassemia minor among men who underwent premarital screening in Quchana city in Khorasan Razavi region of Iran Materials and Methods This research is a descriptive cross-sectional study. From 2010 to 2011, all participants (1000) under marriage coming to health center of Quchan underwent routine mandatory tests. Participants were considered to have beta-thalassemia minor on the condition that hey had a mean corpuscular volume (MCV) 3.5%. Venous blood was taken into an EDTA tube and the complete blood count and red blood cell indices were measured with a Coulter automated cell counter. Electrophoresis was performed on cellulose acetate. Results Mean and SD of hemoglobin, MCV and MCH were 16±2.9, 91±4 and 28.4±2, respectively. Hemoglobin A2 Higher than 3.5 percent was reported as 3.5%.The prevalence of beta-thassemia minor with high hemoglobin A2 and microcytic hypochromic anemia was 3.5% (P-value). Conclusion In countries with high prevalence of hemoglobinopathies, a premarital screening program is helpful for identification and prevention of high-risk marriages. Detecting carrier couples with premarital screening program is an effective way of controlling thalassemia major. PMID:24575266

  14. Transcellular ion flow in Escherichia coli B and electrical sizing of bacterias. (United States)

    Zimmermann, U; Schulz, J; Pilwat, G


    Dielectric breakdown of cell membranes and, in response, transcellular ion flows were measured in Escherichia coli B 163 and B 525 using a Coulter counter as the detector with a hydrodynamic jet focusing close to the orifice of the counter. Plotting the relative pulse height for compensated amplification of a certain size of the cells against increasing detector current, a rather sharp bend within the linear function was found, which did not occur when measuring fixed cells or polystyrene latex. The start current for transcellular ion flow causing the change of the slope is different for the potassium-deficient mutant B 525 in comparison with the wild-type B 163, indicating a change in the membrane structure of B 525 by mutation and demonstrating the sensitivity of the method for studying slight changes in membrane structure in general. The theoretical size distributions for two current values in the range of transcellular ion flow were constructed from the true size distribution at low detector currents, assuming an idealized sharp changeover of the bacterial conductivity from zero to one-third of the electrolyte conductivity.

  15. Neonatal nucleated red blood cells in G6PD deficiency. (United States)

    Yeruchimovich, Mark; Shapira, Boris; Mimouni, Francis B; Dollberg, Shaul


    The objective of this study is to study the absolute number of nucleated red blood cells (RBC) at birth, an index of active fetal erythropoiesis, in infants with G6PD deficiency and in controls. We tested the hypothesis that hematocrit and hemoglobin would be lower, and absolute nucleated RBC counts higher, in the G6PD deficient and that these changes would be more prominent in infants exposed passively to fava bean through maternal diet. Thirty-two term infants with G6PD deficiency were compared with 30 term controls. Complete blood counts with manual differential counts were obtained within 12 hours of life. Absolute nucleated RBC and corrected leukocyte counts were computed from the Coulter results and the differential count. G6PD deficient patients did not differ from controls in terms of gestational age, birth weight, or Apgar scores or in any of the hematologic parameters studied, whether or not the mother reported fava beans consumption in the days prior to delivery. Although intrauterine hemolysis is possible in G6PD deficient fetuses exposed passively to fava beans, our study supports that such events must be very rare.

  16. High-level 13C-enrichment of random and synchronous populations of Chlamydomonas reinhardii

    International Nuclear Information System (INIS)

    Price, R.L.; Crissman, H.A.; Martin, J.C.; Kollman, V.H.


    The alga Chlamydomonas reinhardii was grown in suspension culture at high levels of 13 C-enrichment (98 mol percent) both in synchronous and random populations for the purpose of investigating possible macro- and ultrastructural changes in the cell as induced by essentially total carbon replacement. The algae, grown in spinner flasks, were analyzed using a newly developed multiparameter flow-system technique applied to characterizing various algal genera. The versatility of this technique provides for measuring and processing several cell characteristics simultaneously and separating cells according to selected combinations of parameters. In these studies, cell volume (by Coulter aperture) and DNA and chlorophyll content were determined simultaneously. Cell ultrastructure was examined at various levels of isotope enrichment and time periods by electron microscopy. The data presented for synchronous growth of this organism demonstrate the absence of biological effects (considering the parameters measured) due to the almost total replacement of cellular 12 C with 13 C. Interpretational problems encountered when looking for biological effects on random populations are discussed

  17. Removal of focal atheromatous lesions by angioscopically guided high-speed rotary atherectomy. Preliminary experimental observations

    International Nuclear Information System (INIS)

    Ahn, S.S.; Auth, D.; Marcus, D.R.; Moore, W.S.


    A new high-speed rotary atherectomy device, inserted over a guide wire and directed with an angioscope, offers the potential of restoring patency of outflow vessels by boring out the atheromatous lesion of the orifices of runoff vessels. The device was tested on 68 cadaver arteries with atheromatous lesions involving the superficial femoral, popliteal, and tibial arteries. This was performed with either free segments or in situ with the device placed through a popliteal arteriotomy. The gross results of rotary atherectomy were assessed by angioscopy, angiography, or both. The luminal surfaces were studied with scanning electron microscopy and transverse sections of vessels were studied with light microscopy. The pulverized atheroma, in colloidal suspension, was analyzed for particle size by Coulter counter. The effect of a colloidal suspension of atheromatous particles on distal capillary circulation was measured in animal experiments. Obstructive lesions were successfully removed in 36 of 37 stenotic arteries (97%) and 18 of 31 completely occluded arteries (58%), an overall efficacy of 54 of 68 (79%). In successfully atherectomized arteries, angioscopy and angiography demonstrated a widely patent, smooth, polished surface. Light microscopy demonstrated removal of the diseased intima with maintenance of the outer media and adventitia. The pulverized atheroma particles were generally smaller than red blood cells and injection of the colloidal atheroma into canine femoral arteries failed to produce local tissue injury. We conclude that in the human cadaver this atherectomy device effectively enlarges and recanalizes obstructed superficial femoral, popliteal, and tibial arteries

  18. A Comparison of Platelet Count and Enrichment Percentages in the Platelet Rich Plasma (PRP) Obtained Following Preparation by Three Different Methods. (United States)

    Sabarish, Ram; Lavu, Vamsi; Rao, Suresh Ranga


    Platelet rich plasma (PRP) represents an easily accessible and rich source of autologous growth factors. Different manual methods for the preparation of PRP have been suggested. Lacuna in knowledge exists about the efficacy of PRP preparation by these different manual methods. This study was performed to determine the effects of centrifugation rate revolutions per minute (RPM) and time on the platelet count and enrichment percentages in the concentrates obtained following the three different manual methods of PRP preparation. In vitro experimental study. This was an experimental study in which platelet concentration was assessed in the PRP prepared by three different protocols as suggested by Marx R (method 1), Okuda K (method 2) and Landesberg R (method 3). A total of 60 peripheral blood samples, (n=20 per method) were obtained from healthy volunteers. Baseline platelet count was assessed for all the subjects following which PRP was prepared. The platelet count in the PRP was determined using coulter counter (Sysmex XT 2000i). The mean of the platelet count obtained and their enrichment percentage were calculated and intergroup comparison was done (Tukey's HSD test). The number of platelets and enrichment percentage in PRP prepared by method 1 was higher compared to method 2 and method 3; this difference in platelet concentrates was found to be statistically significant (p < 0.05). The centrifugation rate and time appear to be important parameters, which influence the platelet yield. Method 1 which had lower centrifugation rate and time yielded a greater platelet count and enrichment percentage.

  19. A comparative study of U937 cell size changes during apoptosis initiation by flow cytometry, light scattering, water assay and electronic sizing. (United States)

    Yurinskaya, Valentina; Aksenov, Nikolay; Moshkov, Alexey; Model, Michael; Goryachaya, Tatyana; Vereninov, Alexey


    A decrease in flow cytometric forward light scatter (FSC) is commonly interpreted as a sign of apoptotic cell volume decrease (AVD). However, the intensity of light scattering depends not only on the cell size but also on its other characteristics, such as hydration, which may affect the scattering in the opposite way. That makes estimation of AVD by FSC problematic. Here, we aimed to clarify the relationship between light scattering, cell hydration (assayed by buoyant density) and cell size by the Coulter technique. We used human lymphoid cells U937 exposed to staurosporine, etoposide or hypertonic stress as an apoptotic model. An initial increase in FSC was found to occur in apoptotic cells treated with staurosporine and hypertonic solutions; it is accompanied by cell dehydration and is absent in apoptosis caused by etoposide that is consistent with the lack of dehydration in this case. Thus, the effect of dehydration on the scattering signal outweighs the effect of reduction in cell size. The subsequent FSC decrease, which occurred in parallel to accumulation of annexin-positive cells, was similar in apoptosis caused by all three types of inducers. We conclude that an increase, but not a decrease in light scattering, indicates the initial cell volume decrease associated with apoptotic cell dehydration.

  20. Distribution of In-111 in granulocyte and other cellular elements of blood (CEB) in human In-111-labeled mixed white cell (MWC) and platelet preparations

    International Nuclear Information System (INIS)

    Dewanjee, M.K.; Chowdhury, S.; Brown, M.L.; Wahner, H.W.


    A large number of platelets (PLT), red blood cells (RBC) are present along with granulocyte (GC) in In-111 in CEB was determined by Ficoll-Hypaque gradient (FHG) centrifugation of In-111-MWC and PLT preparation as a quality control procedure. MWC were separated by sedimentation with hydroxyethyl starch; PLT by differential centrifugation. MWC and PLT were labeled with In-111-oxine in saline, ACD-saline or with In-111-tropolone in 0.5 ml of ACD-plasma. 0.3-0.5 ml of labeled cell suspended in plasma was layered on 3 ml FHG of two densities (1.119 and 1.077 gm/ml) and spun in a clear polystyrene tube at 1800 G for 30 min. Four layers (plasma, PLT, GC, and RBC) were separated, and In-111 radioactivity in each fraction was determined with a gamma counter. Simultaneously cell types in MWC and PLT preparations were determined by Coulter counter and differential counting. Most of In-111 in In-MWC is associated with the PLT and RBC, GC/lymphocyte ratio is 6/4. GC has higher extraction efficiency than RBC and PLT. PLT preparation is pure and (96 +- 3)% of In-111 is bound to PLT, (4 +- 3)% to RBC and (0.2 +- 0.1)% to GC; PLT preparation contains PLT (97 +- 3)%, RBC (4 +- 3)% and GC (0.2 +- 0.1)%

  1. Decontamination of materials contaminated with Bacillus anthracis and Bacillus thuringiensis Al Hakam spores using PES-Solid, a solid source of peracetic acid. (United States)

    Buhr, T L; Wells, C M; Young, A A; Minter, Z A; Johnson, C A; Payne, A N; McPherson, D C


    To develop test methods and evaluate survival of Bacillus anthracis Ames, B. anthracis ∆Sterne and B. thuringiensis Al Hakam spores after exposure to PES-Solid (a solid source of peracetic acid), including PES-Solid formulations with bacteriostatic surfactants. Spores (≥ 7 logs) were dried on seven different test materials and treated with three different PES-Solid formulations (or preneutralized controls) at room temperature for 15 min. There was either no spore survival or less than 1 log (<10 spores) of spore survival in 56 of 63 test combinations (strain, formulation and substrate). Less than 2.7 logs (<180 spores) survived in the remaining seven test combinations. The highest spore survival rates were seen on water-dispersible chemical agent resistant coating (CARC-W) and Naval ship topcoat (NTC). Electron microscopy and Coulter analysis showed that all spore structures were intact after spore inactivation with PES-Solid. Three PES-Solid formulations inactivated Bacillus spores that were dried on seven different materials. A test method was developed to show that PES-Solid formulations effectively inactivate Bacillus spores on different materials. Published 2013. This article is a U.S. Government work and is in the public domain in the USA.

  2. Microfluidic CODES: a scalable multiplexed electronic sensor for orthogonal detection of particles in microfluidic channels. (United States)

    Liu, Ruxiu; Wang, Ningquan; Kamili, Farhan; Sarioglu, A Fatih


    Numerous biophysical and biochemical assays rely on spatial manipulation of particles/cells as they are processed on lab-on-a-chip devices. Analysis of spatially distributed particles on these devices typically requires microscopy negating the cost and size advantages of microfluidic assays. In this paper, we introduce a scalable electronic sensor technology, called microfluidic CODES, that utilizes resistive pulse sensing to orthogonally detect particles in multiple microfluidic channels from a single electrical output. Combining the techniques from telecommunications and microfluidics, we route three coplanar electrodes on a glass substrate to create multiple Coulter counters producing distinct orthogonal digital codes when they detect particles. We specifically design a digital code set using the mathematical principles of Code Division Multiple Access (CDMA) telecommunication networks and can decode signals from different microfluidic channels with >90% accuracy through computation even if these signals overlap. As a proof of principle, we use this technology to detect human ovarian cancer cells in four different microfluidic channels fabricated using soft lithography. Microfluidic CODES offers a simple, all-electronic interface that is well suited to create integrated, low-cost lab-on-a-chip devices for cell- or particle-based assays in resource-limited settings.

  3. Microfluidic Transducer for Detecting Nanomechanical Movements of Bacteria (United States)

    Kara, Vural; Ekinci, Kamil


    Various nanomechanical movements of bacteria are currently being explored as an indication of bacterial viability. Most notably, these movements have been observed to subside rapidly and dramatically when the bacteria are exposed to an effective antibiotic. This suggests that monitoring bacterial movements, if performed with high fidelity, can offer a path to various clinical microbiological applications, including antibiotic susceptibility tests. Here, we introduce a robust and sensitive microfluidic transduction technique for detecting the nanomechanical movements of bacteria. The technique is based on measuring the electrical fluctuations in a microchannel which the bacteria populate. These electrical fluctuations are caused by the swimming of motile, planktonic bacteria and random oscillations of surface-immobilized bacteria. The technique provides enough sensitivity to detect even the slightest movements of a single cell and lends itself to smooth integration with other microfluidic methods and devices; it may eventually be used for rapid antibiotic susceptibility testing. We acknowledge support from Boston University Office of Technology Development, Boston University College of Engineering, NIH (1R03AI126168-01) and The Wallace H. Coulter Foundation.

  4. Effect of animal sera on Bacillus anthracis Sterne spore germination and vegetative cell growth. (United States)

    Bensman, M D; Mackie, R S; Minter, Z A; Gutting, B W


     The aims of this work were to investigate the effects of sera on B. anthracis Sterne germination and growth. Sera examined included human, monkey and rabbit sera, as well as sera from eight other species.  Standard dilution plate assay (with and without heat kill) was used as a measure of germination, and spectroscopy was used to measure growth. In addition, a Coulter Counter particle counter was used to monitor germination and growth based on bacterial size. Spores germinated best in foetal bovine and monkey sera, moderately with human sera and showed limited germination in the presence of rabbit or rat sera. Vegetative bacteria grew best in foetal bovine sera and moderately in rabbit sera. Human and monkey sera supported little growth of vegetative bacteria.  The data suggested sera can have a significant impact on germination and growth of Sterne bacteria.  These data should be considered when conducting in vitro cell culture studies and may aid in interpreting in vivo infection studies. © 2012 The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.

  5. Using Fluorescence Intensity of Enhanced Green Fluorescent Protein to Quantify Pseudomonas aeruginosa

    Directory of Open Access Journals (Sweden)

    Erin Wilson


    Full Text Available A variety of direct and indirect methods have been used to quantify planktonic and biofilm bacterial cells. Direct counting methods to determine the total number of cells include plate counts, microscopic cell counts, Coulter cell counting, flow cytometry, and fluorescence microscopy. However, indirect methods are often used to supplement direct cell counting, as they are often more convenient, less time-consuming, and require less material, while providing a number that can be related to the direct cell count. Herein, an indirect method is presented that uses fluorescence emission intensity as a proxy marker for studying bacterial accumulation. A clinical strain of Pseudomonas aeruginosa was genetically modified to express a green fluorescent protein (PA14/EGFP. The fluorescence intensity of EGFP in live cells was used as an indirect measure of live cell density, and was compared with the traditional cell counting methods of optical density (OD600 and plate counting (colony-forming units (CFUs. While both OD600 and CFUs are well-established methods, the use of fluorescence spectroscopy to quantify bacteria is less common. This study demonstrates that EGFP intensity is a convenient reporter for bacterial quantification. In addition, we demonstrate the potential for fluorescence spectroscopy to be used to measure the quantity of PA14/EGFP biofilms, which have important human health implications due to their antimicrobial resistance. Therefore, fluorescence spectroscopy could serve as an alternative or complementary quick assay to quantify bacteria in planktonic cultures and biofilms.

  6. Prehistoric peyote use: alkaloid analysis and radiocarbon dating of archaeological specimens of Lophophora from Texas. (United States)

    El-Seedi, Hesham R; De Smet, Peter A G M; Beck, Olof; Possnert, Göran; Bruhn, Jan G


    Two archaeological specimens of peyote buttons, i.e. dried tops of the cactus Lophophora williamsii (Lem.) Coulter, from the collection of the Witte Museum in San Antonio, was subjected to radiocarbon dating and alkaloid analysis. The samples were presumably found in Shumla Cave No. 5 on the Rio Grande, Texas. Radiocarbon dating shows that the calibrated 14C age of the weighted mean of the two individual dated samples corresponds to the calendric time interval 3780-3660 BC (one sigma significance). Alkaloid extraction yielded approximately 2% of alkaloids. Analysis with thin-layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS) led to the identification of mescaline in both samples. No other peyote alkaloids could be identified. The two peyote samples appear to be the oldest plant drug ever to yield a major bioactive compound upon chemical analysis. The identification of mescaline strengthens the evidence that native North Americans recognized the psychotropic properties of peyote as long as 5700 years ago.

  7. An iterative procedure for estimating areally averaged heat flux using planetary boundary layer mixed layer height and locally measured heat flux

    Energy Technology Data Exchange (ETDEWEB)

    Coulter, R. L.; Gao, W.; Lesht, B. M.


    Measurements at the central facility of the Southern Great Plains (SGP) Cloud and Radiation Testbed (CART) are intended to verify, improve, and develop parameterizations in radiative flux models that are subsequently used in General Circulation Models (GCMs). The reliability of this approach depends upon the representativeness of the local measurements at the central facility for the site as a whole or on how these measurements can be interpreted so as to accurately represent increasingly large scales. The variation of surface energy budget terms over the SGP CART site is extremely large. Surface layer measurements of the sensible heat flux (H) often vary by a factor of 2 or more at the CART site (Coulter et al. 1996). The Planetary Boundary Layer (PBL) effectively integrates the local inputs across large scales; because the mixed layer height (h) is principally driven by H, it can, in principal, be used for estimates of surface heat flux over scales on the order of tens of kilometers. By combining measurements of h from radiosondes or radar wind profiles with a one-dimensional model of mixed layer height, they are investigating the ability of diagnosing large-scale heat fluxes. The authors have developed a procedure using the model described by Boers et al. (1984) to investigate the effect of changes in surface sensible heat flux on the mixed layer height. The objective of the study is to invert the sense of the model.

  8. Regrowth and radiation sensitivity of quiescent cells isolated from EMT6/Ro-fed plateau monolayers

    International Nuclear Information System (INIS)

    Luk, C.K.; Keng, P.C.; Sutherland, R.M.


    A quiescent [denoted as Q(G0/G1)] subpopulation was isolated from EMT6/Ro-fed plateau monolayers by centrifugal elutriation. The median Coulter volume of these cells was significantly smaller than that of the original population from which they were elutriated. Using two-step acridine orange staining and dual parameter flow cytometric analysis, over 95% of quiescent cells were found to have G1 DNA content, and 80% of the cells had a decreased RNA content as compared to rapidly proliferating exponential G1 cells. After labeling for 24 hr (two doubling times) with [ 3 H]thymidine, less than 2% of the quiescent cells incorporated [3H]thymidine as measured by autoradiography. The colony-forming efficiency of these cells was not significantly different from that of exponential cells. When such Q(G0/G1) cells were replated in fresh medium at a lower density, there was a lag time of 30 hr before any increase in cell number was detected, after which the cell-doubling rate matched that of exponential culture. Results obtained from the radiation dose-response curves showed that quiescent (G0/G1) cells were more radiosensitive than exponential G1 or unseparated fed plateau cells

  9. Prevalence of anemia and deficiencies of iron, folic acid and vitamin B12 in an indigenous community from the Venezuelan Amazon with a high incidence of malaria. (United States)

    García-Casal, Maria Nieves; Leets, Irene; Bracho, Carmen; Hidalgo, Mariana; Bastidas, Gilberto; Gomez, Ana; Peña, Ana; Pérez, Hilda


    The objective of this work was to determine the prevalence of anemia and deficiencies of iron, folic acid and vitamin B12 in Betania del Topocho, a Piaroa community from Estado Amazonas, Venezuela, a zone with a high incidence of malaria. The group studied included 184 subjects of all ages that assisted to the local health center for malaria diagnosis. Analysis performed included hematology by coulter counter, ferritin quantification by ELISA with monoclonal antibodies and folic acid and vitamin B12 determinations by an immunoradiometric assay. It was found that the prevalence of anemia was 89.6% and deficiencies of iron, folic acid and vitamin B12 affected 37.1,70.3 and 12.4% of the population studied, respectively. Plasmodium infection was detected by molecular diagnosis in 53.2% of the cases, and 86% of them were anemic. The highest incidence of anemia was found in children, with a prevalence of 100% in infants of both sexes. The high prevalence of anemia, iron and folic acid deficiencies found, indicates an important health and nutrition problem that should be immediately and properly addressed. The number of cases of anemia due to iron deficiency could be underestimated, since ferritin concentration increased as a acute phase protein, although prevalence data was also analyzed with a cutoff point of 30 microg/L for ferritin concentration.

  10. Mineral Analysis of Pine Nuts (Pinus spp.) Grown in New Zealand. (United States)

    Vanhanen, Leo P; Savage, Geoffrey P


    Mineral analysis of seven Pinus species grown in different regions of New Zealand; Armand pine ( Pinus armandii Franch), Swiss stone pine ( Pinus cembra L.), Mexican pinyon ( Pinus cembroides Zucc. var. bicolor Little), Coulter pine ( Pinus coulteri D. Don), Johann's pine ( Pinus johannis M.F. Robert), Italian stone pine ( Pinus pinea L.) and Torrey pine ( Pinus torreyana Parry ex Carrière), was carried out using an inductively coupled plasma optical emission spectrophotometer (ICP-OES) analysis. Fourteen different minerals (Al, B, Ca, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, S and Zn) were identified in all seven varieties, except that no Al or Na was found in Pinus coulteri D. Don. New Zealand grown pine nuts are a good source of Cu, Mg, Mn, P and Zn, meeting or exceeding the recommended RDI for these minerals (based on an intake of 50 g nuts/day) while they supplied between 39%-89% of the New Zealand RDI for Fe. Compared to other commonly eaten tree-nuts New Zealand grown pine nuts are an excellent source of essential minerals.

  11. Crystallization of calcium oxalate in minimally diluted urine (United States)

    Bretherton, T.; Rodgers, A.


    Crystallization of calcium oxalate was studied in minimally diluted (92%) urine using a mixed suspension mixed product crystallizer in series with a Malvern particle sizer. The crystallization was initiated by constant flow of aqueous sodium oxalate and urine into the reaction vessel via two independent feed lines. Because the Malvern cell was in series with the reaction vessel, noninvasive measurement of particle sizes could be effected. In addition, aliquots of the mixed suspension were withdrawn and transferred to a Coulter counter for crystal counting and sizing. Steady-state particle size distributions were used to determine nucleation and growth kinetics while scanning electron microscopy was used to examine deposited crystals. Two sets of experiments were performed. In the first, the effect of the concentration of the exogenous sodium oxalate was investigated while in the second, the effect of temperature was studied. Calcium oxalate nucleation and growth rates were found to be dependent on supersaturation levels inside the crystallizer. However, while growth rate increased with increasing temperature, nucleation rates decreased. The favored phases were the trihydrate at 18°C, the dihydrate at 38° and the monohydrate at 58°C. The results of both experiments are in agreement with those obtained in other studies that have been conducted in synthetic and in maximally diluted urine and which have employed invasive crystal counting and sizing techniques. As such, the present study lends confidence to the models of urinary calcium oxalate crystallization processes which currently prevail in the literature.

  12. Flow cytometry and integrated imaging

    Directory of Open Access Journals (Sweden)

    V. Kachel


    Full Text Available It is a serious problem to relate the results of a flow cytometric analysis of a marine sample to different species. Images of particles selectively triggered by the flow cytometric analysis and picked out from the flowing stream give a valuable additional information on the analyzed organisms. The technical principles and problems of triggered imaging in flow are discussed, as well as the positioning of the particles in the plane of focus, freezing the motion of the quickly moving objects and what kinds of light sources are suitable for pulsed illumination. The images have to be stored either by film or electronically. The features of camera targets and the memory requirements for storing the image data and the conditions for the triggering device are shown. A brief explanation of the features of three realized flow cytometric imaging (FCI systems is given: the Macro Flow Planktometer built within the EUROMAR MAROPT project, the Imaging Module of the European Plankton Analysis System, supported by the MAST II EurOPA project and the most recently developed FLUVO VI universal flow cytometer including HBO 100- and laser excitation for fluorescence and scatter, Coulter sizing as well as bright field and and phase contrast FCI.

  13. [Preparation and preliminary evaluation of KGDS-targeted ultrasound contrast agent]. (United States)

    Gao, Feng; Ding, Yanfei; Sheng, Xiaoxi; Wang, Wei; Liang, Qi; Luo, Zhuoqiong; Zhou, Ping; Li, Hui


    To prepare a thrombus-targeted ultrasonic contrast agent and to investigate its targeted ability to fresh blood clots. We first synthesized FITC-KGDS-Palm compound, and then prepared thrombus-targeted microbubbles using "ultrasound & high speed shearing method". Fluorescence labeling thrombus-specific peptides and KGDS, directed at the activated glycoprotein(GP)IIb/IIIa receptor of platelets were attached to the surface of lipid microbubbles. The concentration and size of TUCA were measured by Malvern Zeta Sizer Nano-ZS590 and Coulter counter. Immunofluorescence was applied to confirm the conjugation. The conjunct ratio was assessed by flow cytometer (FCM). The KGDS-TUCA was straw yellow turbid liquor, and the concentration was 1.5 x 10(9)/mL, and the average size was 1.5 microm. The targeted microbubbles conjugated with the thrombus-specific peptides showed bright green rings by fluorescence microscope. FCM demonstrated that the wavelength of shell of KGDS-TUCA changed greatly, and the conjunct ratio was 90.04%. In vitro study showed KGDS-TUCA remained stable for 48 h at 4 degree C and target-attached to blood clots and showed good stability. The ultrasound & high speed shearing method to prepare TUCA is easy and in favor of purification. KGDS-TUCA has high specific biological activity. The conjunct ratio and stability of KGDS-TUCA are excellent.

  14. Phytoremediation of disturbed lands in Polar Regions of Russia (United States)

    Shamsutdinova, Evgeniya


    In the Northern regions the problem of restoration of disturbed lands as a result of anthropogenic activities is particularly acut. A large role for the success of native plants to take hold and re-establish themselves is to allow the natural process to work without human intervention. However observation shows that low reserves of available moisture, poor soil nutrients, exposure to wind and water erosion make impossible of a complete restoration of lands due to natural revegetation. One of the methods used to minimize the effect of these factors is the phytoremediation. Research on the Pelyatinskom gas condensate field in the Taimyr Dolgano-Nenets municipal district revealed the efficacy of biological remediation of awnless brome, wildrye siberian, red fescue and kentucky bluegrass. Geobotanical studies of the tailings fields in Yakutia showed that for the phytoremediation of tailings of alluvial deposits of diamonds slough grass can be successfully used, and on the dumps of coal deposits with a high degree of survival of the willow, poplar, larch, alder stand and pine. Development of technology for remediation of placer gold deposits of the Komsomol mine and Bilibino in Chukotka has shown the effectiveness of sowing common oat, wildrye and larch. The study of the experience of recultivation on objects of the "Transneft-Baltic" in the North-Western Federal district allowed us to select as the recommended mixtures of species: meadow fescue, cocksfoot, timothy grass, white and alsike clover and alfalfa. The multicomponent mixture of red fescue, awnless brome, meadow fescue, timothy grass, couch grass, kentucky bluegrass, beckman's grass were successfully used at the mine of the Bovanenkovo oil and gas condensate field. On the technological grounds of the complex pumping station in the Yamalo-Nenets Autonomous area willows were planted. Pine and willow trees were planted on quarry workings in the Northern taiga of Western Siberia land .Planting of pines has shown

  15. Rapid detection of acute kidney injury by urinary neutrophil gelatinase-associated lipocalin after cardiopulmonary bypass surgery

    International Nuclear Information System (INIS)

    Munir, M.U.; Khan, D.A.; Khan, F.A.; Naqvi, S.M.S.


    Objective: To determine the accuracy of neutrophil gelatinase-associated lipocalin (NGAL) in early detection of acute kidney injury (AKI) after cardiopulmonary bypass (CPB) surgery by comparing with serum creatinine. Study Design: Descriptive study. Place and Duration of Study: Department of Chemical Pathology and Endocrinology, AFIP in collaboration with AFIC/ NIHD, Rawalpindi, from April to December 2011. Methodology: Eighty eight patients undergoing CPB surgery in AFIC/NIHD were included by consecutive sampling. Blood samples of subjects for serum creatinine analysis were drawn pre-operatively, 4 h, 24 h and 48 h after CPB surgery. Spot urine samples for NGAL were collected at 4 h after CPB surgery. Urine samples were analyzed on Abbott ARCHITECT i2000SR analyzer whereas serum creatinine samples were measured on Beckman UniCel DxC 600 Synchron Clinical System. Results: Out of 88 patients, 11 (13%) cases developed AKI 4 h postoperatively. Urinary NGAL increased markedly at 4 h postoperatively as compared to serum creatinine which showed rise at 24 - 48 h after cardiac surgery. Analysis of urine NGAL at a cutoff value of 87 ng/ml showed area under the curve of 0.91 [95% confidence interval (CI) 0.83 - 0.96] with sensitivity of 90.9% (95% CI 58.7 - 98.5) and specificity of 98.7% (95% CI 92.9-99.8). There was a positive correlation of 4 h urine NGAL and serum delta creatinine at 48 h, which was statistically significant (rs = 0.33, p = 0.001). Conclusion: The study demonstrated that levels of urine NGAL in patients suffering from AKI increased significantly at 4 h as compared to serum creatinine levels. Urine NGAL is an early predictive biomarker of AKI after CPB. (author)

  16. Post-standardization of routine creatinine assays: are they suitable for clinical applications. (United States)

    Jassam, Nuthar; Weykamp, Cas; Thomas, Annette; Secchiero, Sandra; Sciacovelli, Laura; Plebani, Mario; Thelen, Marc; Cobbaert, Christa; Perich, Carmen; Ricós, Carmen; Paula, Faria A; Barth, Julian H


    Introduction Reliable serum creatinine measurements are of vital importance for the correct classification of chronic kidney disease and early identification of kidney injury. The National Kidney Disease Education Programme working group and other groups have defined clinically acceptable analytical limits for creatinine methods. The aim of this study was to re-evaluate the performance of routine creatinine methods in the light of these defined limits so as to assess their suitability for clinical practice. Method In collaboration with the Dutch External Quality Assurance scheme, six frozen commutable samples, with a creatinine concentration ranging from 80 to 239  μmol/L and traceable to isotope dilution mass spectrometry, were circulated to 91 laboratories in four European countries for creatinine measurement and estimated glomerular filtration rate calculation. Two out of the six samples were spiked with glucose to give high and low final concentrations of glucose. Results Results from 89 laboratories were analysed for bias, imprecision (%CV) for each creatinine assay and total error for estimated glomerular filtration rate. The participating laboratories used analytical instruments from four manufacturers; Abbott, Beckman, Roche and Siemens. All enzymatic methods in this study complied with the National Kidney Disease Education Programme working group recommended limits of bias of 5% above a creatinine concentration of 100  μmol/L. They also did not show any evidence of interference from glucose. In addition, they also showed compliance with the clinically recommended %CV of ≤4% across the analytical range. In contrast, the Jaffe methods showed variable performance with regard to the interference of glucose and unsatisfactory bias and precision. Conclusion Jaffe-based creatinine methods still exhibit considerable analytical variability in terms of bias, imprecision and lack of specificity, and this variability brings into question their clinical utility

  17. Kearns–Sayre syndrome: a case series of 35 adults and children

    Directory of Open Access Journals (Sweden)

    Khambatta S


    Full Text Available Sherezade Khambatta, Douglas L Nguyen, Thomas J Beckman, Christopher M Wittich Division of General Internal Medicine, Mayo Clinic, Rochester, MN, USA Background: Kearns–Sayre syndrome (KSS is a rare mitochondrial cytopathy, first described at Mayo Clinic in 1958. Aims: We aimed to define patient and disease characteristics in a large group of adult and pediatric patients with KSS. Methods: We retrospectively searched the Mayo Clinic medical index patient database for the records of patients with KSS between 1976 and 2009. The 35 patients identified with KSS were analyzed in terms of demographic characteristics, presenting signs and symptoms, diagnostic features, clinical evolution, and associations between disease features and the development of disability. Results: The mean (standard [SD] age at KSS presentation was 17 (10 years, but the mean age at diagnosis was 26 (15 years. Ophthalmologic symptoms developed in all patients, and neurologic and cardiac involvement was common. Only four patients (11% in the series died, but all deaths were from sudden cardiac events. The development of physical disability was significantly associated with cognitive decline (P=0.004 but not with other clinical features, such as sex or sudden cardiac death. Conclusion: We report the largest case series to date of patients with KSS from a single institution. In addition to the conduction system abnormalities identified in previous series, our cohort included patients with syncope and sudden cardiac death. This underscores the need to consider formal electrophysiologic studies and prophylactic defibrillators in patients with KSS. Keywords: heart block, mitochondrial diseases, ophthalmoplegia, retinitis pigmentosa

  18. Evaluation of a third party enzymatic ammonia method for use on the Roche Cobas 6000 (c501) automated platform. (United States)

    Seiden-Long, Isolde; Schnabl, Kareena; Skoropadyk, Wendy; Lennon, Nola; McKeage, Arlayne


    Adaptation of the Randox Enzymatic Manual UV Ammonia method to be used on the Roche Cobas 6000 (c501) automated analyzer platform. The Randox ammonia reagent was evaluated for precision, linearity, accuracy and interference from hemolysis, icterus and lipemia on the Roche c501 analyzer. Comparison studies were conducted for the Randox reagent between Roche c501, Siemens Vista, Ortho Vitros 250, and Beckman DxC methods. The Randox reagent demonstrates acceptable within-run (L1=65 μmol/L, CV 3.4% L2=168 μmol/L, CV 1.9%) and between-run precision (L1=29 μmol/L, CV 7.3% L2=102 μmol/L, CV 3.0%), Analytical Measurement Range (7-940 μmol/L), and accuracy. The method interference profile is superior for the Randox method (hemolysis index up to 600, icteric index up to 60, lipemic index up to 100) as compared to the Roche method (hemolysis index up to 200, icteric index up to 10, lipemic index up to 50). Comparison was very good between the Randox reagent and two other wet chemistry platforms. The Randox Enzymatic Manual UV Ammonia reagent is an available alternative to the Roche Cobas c501 reagent. The method is more robust to endogenous interferences and less prone to instrument error flags, thus allowing the majority of clinical specimens to be reported without additional sample handling at our institution. Copyright © 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  19. Utilization of {sup 15}N-Diammonium Phosphate by Ruminants to Produce Milk and Meat Proteins

    Energy Technology Data Exchange (ETDEWEB)

    Piva, G.; Silva, S. [Istituto di Zootecnicae di Chimica Agraria, Facolta di Agraria Univ. Cattolicas. Cuore, Piacenza (Italy)


    The authors investigated the alimentary role of diammonium phosphate (DAP) in ruminants. For this study DAP labelled with {sup 15}N was used; analysis of the {sup 15}N atomic per cent excess was made with an Italelettronica mass spectrophotometer (model SP 21 F) and the amino acid determination by a Beckman-Spinco amino acid analyser (model 120B) fitted with a preparative column. For the experiment 7 g of DAP at 15 and 20 at. % excess {sup 15}N were administered once to mature lactating and non-lactating sheep, respectively. The measurement of {sup 15}N in the protein and isolated amino acids of milk and meat showed: (1) The milk protein produced in the first 24 h contained the highest atomic per cent excess of {sup 15}SN, 0.093; (2) That the supplemental {sup 15}N was found in all the amino acids of milk proteins except tryptophane. The atomic per cent excess of {sup 15}N was observed to vary between the various amino acids. These results confirmed previous observations on bacterial protein synthesized from DAP. (3) Muscle protein {sup 15}N maximized on the third day after administration of the {sup 15}N-DAP, with an atomic per cent excess of 0.040; (4) The atomic per cent excess of {sup 15}N in the individual amino acids of muscle protein is significant in only two amino' acids, serine and cystine; and (5) That after 8 d of adaptation there are no traces of DAP in milk or meat proteins, urine or faeces. The authors conclude that the ruminant, after a period of adaptation and through the mediation of ruminant microorganisms, is able to use the nitrogen of diammonium phosphate for the synthesis of milk and meat proteins. (author)

  20. Enhanced radiation response in radioresistant MCF-7 cells by targeting peroxiredoxin II

    Directory of Open Access Journals (Sweden)

    Diaz AJG


    Full Text Available Anthony Joseph Gomez Diaz,1 Daniel Tamae,2 Yun Yen,3 JianJian Li,4 Tieli Wang1 1Department of Chemistry and Biochemistry, California State University at Dominguez Hills, Carson, CA, 2Center of Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania, Philadelphia, PA, 3Department of Clinical and Molecular Pharmacology, Beckman Research Institute of City of Hope National Medical Center, Duarte, CA, 4Department of Radiation Oncology, University of California Davis, Sacramento, CA, USA Abstract: In our previous study, we identified that a protein target, peroxiredoxin II (PrxII, is overexpressed in radioresistant MCF+FIR3 breast-cancer cells and found that its expression and function is associated with breast-cancer radiation sensitivity or resistance. Small interference RNA (siRNA targeting PrxII gene expression was able to sensitize MCF+FIR3 radioresistant breast-cancer cells to ionizing radiation. The major focus of this work was to investigate how the radiation response of MCF+FIR3 radioresistant cells was affected by the siRNA that inhibits PrxII gene expression. Our results, presented here, show that silencing PrxII gene expression increased cellular toxicity by altering cellular thiol status, inhibiting Ca2+ efflux from the cells, and perturbing the intracellular Ca2+ homeostasis. By combining radiotherapy and siRNA technology, we hope to develop new therapeutic strategies that may have potential to enhance the efficacy of chemotherapeutic agents due to this technology's property of targeting to specific cancer-related genes. Keywords: siRNA, PrxII, radiation resistance, Ca2+, MCF+FIR3

  1. Measurement of isotopic uranium in water for compliance monitoring by liquid scintillation counting with alpha/beta discrimination

    International Nuclear Information System (INIS)

    Venso, E.A.S.


    A simple and inexpensive method is described for analysis of uranium (U) activity and mass in water by liquid scintillation counting using α/β discrimination. This method appears to offer a solution to the need for an inexpensive protocol for monitoring U activity and mass simultaneously and an alternative to the potential inaccuracy involved when depending on the mass-to-activity conversion factor or activity screen. U is extracted virtually quantitatively into 20 ml extractive scintillator from a 1-ell aliquot of water acidified to less than pH 2. After phase separation, the sample is counted for a 20-minute screening count with a minimum detection level of 0.27 pCi ell -1 . α-particle emissions from the extracted U are counted with close to 100% efficiency with a Beckman LS6000 LL liquid scintillation counter equipped with pulse-shape discrimination electronics. Samples with activities higher than 10 pCi ell -1 are recounted for 500-1000 minutes for isotopic analysis. Isotopic analysis uses events that are automatically stored in spectral files and transferred to a computer during assay. The data can be transferred to a commercially available spreadsheet and retrieved for examination or data manipulation. Values for three readily observable spectral features can be rapidly identified by data examination and substituted into a simple formula to obtain 234 U/ 238 U ratio for most samples. U mass is calculated by substituting the isotopic ratio value into a simple equation. The utility of this method for the proposed compliance monitoring of U in public drinking water supplies was field tested with a survey of drinking water from Texas supplies that had previously been known to contain elevated levels of gross α activity. U concentrations in 32 samples from 27 drinking water supplies ranged from 0.26 to 65.5 pCi ell -1 , with seven samples exceeding the proposed Maximum Contaminant Level

  2. Elevated free nitrotyrosine levels, but not protein-bound nitrotyrosine or hydroxyl radicals, throughout amyotrophic lateral sclerosis (ALS)-like disease implicate tyrosine nitration as an aberrant in vivo property of one familial ALS-linked superoxide dismutase 1 mutant. (United States)

    Bruijn, L I; Beal, M F; Becher, M W; Schulz, J B; Wong, P C; Price, D L; Cleveland, D W


    Mutations in superoxide dismutase 1 (SOD1; EC are responsible for a proportion of familial amyotrophic lateral sclerosis (ALS) through acquisition of an as-yet-unidentified toxic property or properties. Two proposed possibilities are that toxicity may arise from imperfectly folded mutant SOD1 catalyzing the nitration of tyrosines [Beckman, J. S., Carson, M., Smith, C. D. & Koppenol, W. H. (1993) Nature (London) 364, 584] through use of peroxynitrite or from peroxidation arising from elevated production of hydroxyl radicals through use of hydrogen peroxide as a substrate [Wiedau-Pazos, M., Goto, J. J., Rabizadeh, S., Gralla, E. D., Roe, J. A., Valentine, J. S. & Bredesen, D. E. (1996) Science 271, 515-518]. To test these possibilities, levels of nitrotyrosine and markers for hydroxyl radical formation were measured in two lines of transgenic mice that develop progressive motor neuron disease from expressing human familial ALS-linked SOD1 mutation G37R. Relative to normal mice or mice expressing high levels of wild-type human SOD1, 3-nitrotyrosine levels were elevated by 2- to 3-fold in spinal cords coincident with the earliest pathological abnormalities and remained elevated in spinal cord throughout progression of disease. However, no increases in protein-bound nitrotyrosine were found during any stage of SOD1-mutant-mediated disease in mice or at end stage of sporadic or SOD1-mediated familial human ALS. When salicylate trapping of hydroxyl radicals and measurement of levels of malondialdehyde were used, there was no evidence throughout disease progression in mice for enhanced production of hydroxyl radicals or lipid peroxidation, respectively. The presence of elevated nitrotyrosine levels beginning at the earliest stages of cellular pathology and continuing throughout progression of disease demonstrates that tyrosine nitration is one in vivo aberrant property of this ALS-linked SOD1 mutant.

  3. CLSI-based transference and verification of CALIPER pediatric reference intervals for 29 Ortho VITROS 5600 chemistry assays. (United States)

    Higgins, Victoria; Truong, Dorothy; Woroch, Amy; Chan, Man Khun; Tahmasebi, Houman; Adeli, Khosrow


    Evidence-based reference intervals (RIs) are essential to accurately interpret pediatric laboratory test results. To fill gaps in pediatric RIs, the Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) project developed an age- and sex-specific pediatric RI database based on healthy pediatric subjects. Originally established for Abbott ARCHITECT assays, CALIPER RIs were transferred to assays on Beckman, Roche, Siemens, and Ortho analytical platforms. This study provides transferred reference intervals for 29 biochemical assays for the Ortho VITROS 5600 Chemistry System (Ortho). Based on Clinical Laboratory Standards Institute (CLSI) guidelines, a method comparison analysis was performed by measuring approximately 200 patient serum samples using Abbott and Ortho assays. The equation of the line of best fit was calculated and the appropriateness of the linear model was assessed. This equation was used to transfer RIs from Abbott to Ortho assays. Transferred RIs were verified using 84 healthy pediatric serum samples from the CALIPER cohort. RIs for most chemistry analytes successfully transferred from Abbott to Ortho assays. Calcium and CO 2 did not meet statistical criteria for transference (r 2 reference intervals, 29 successfully verified with approximately 90% of results from reference samples falling within transferred confidence limits. Transferred RIs for total bilirubin, magnesium, and LDH did not meet verification criteria and are not reported. This study broadens the utility of the CALIPER pediatric RI database to laboratories using Ortho VITROS 5600 biochemical assays. Clinical laboratories should verify CALIPER reference intervals for their specific analytical platform and local population as recommended by CLSI. Copyright © 2018 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  4. Stop consonant voicing in young children's speech: Evidence from a cross-sectional study (United States)

    Ganser, Emily

    There are intuitive reasons to believe that speech-sound acquisition and language acquisition should be related in development. Surprisingly, only recently has research begun to parse just how the two might be related. This study investigated possible correlations between speech-sound acquisition and language acquisition, as part of a large-scale, longitudinal study of the relationship between different types of phonological development and vocabulary growth in the preschool years. Productions of voiced and voiceless stop-initial words were recorded from 96 children aged 28-39 months. Voice Onset Time (VOT, in ms) for each token context was calculated. A mixed-model logistic regression was calculated which predicted whether the sound was intended to be voiced or voiceless based on its VOT. This model estimated the slopes of the logistic function for each child. This slope was referred to as Robustness of Contrast (based on Holliday, Reidy, Beckman, and Edwards, 2015), defined as being the degree of categorical differentiation between the production of two speech sounds or classes of sounds, in this case, voiced and voiceless stops. Results showed a wide range of slopes for individual children, suggesting that slope-derived Robustness of Contrast could be a viable means of measuring a child's acquisition of the voicing contrast. Robustness of Contrast was then compared to traditional measures of speech and language skills to investigate whether there was any correlation between the production of stop voicing and broader measures of speech and language development. The Robustness of Contrast measure was found to correlate with all individual measures of speech and language, suggesting that it might indeed be predictive of later language skills.

  5. Use of an external source (60Co) for 32P detection efficiency determination by the Cerenkov effect, in soil extracts

    International Nuclear Information System (INIS)

    Nascimento Filho, V.F. do.


    The detection of 32 P in aqueous extracts is usually made with the aid of a Geiger-Muller detector, with thin window and sample on a planchet. Presently the technique is being developed of detection of high energy beta particles emitters ( 32 P, 42 K, 86 Rb) through the Cerenkov effect, using a commercial liquid scintillation system. This technique, despite being approximately 30 times more sensitive, has the inconvenience of varying the detection efficiency, mainly for color samples (soil extracts, for instance). From this stems the need for determining the detection efficiency for each sample. The internal standardization and channels ratio methods show a series of drawbacks, mainly the non-reutilization of the samples (1st method) and statistical uncertainty for low activity samples (2nd method). The elimination of these dreawbacks can be achieved through the utilization of the external standardization method. A 60 Co source with 1,4 μCi activity has been adapted to the sample elevator of the detector system, and a comparison was made with the channels ratio method to evaluate the efficiency of 32 P detection in soil extracts (P extraction and fractionation). The external standardization method showed to be more accurate, besides being influenced to a lesser degree by high voltage variation, sample volume and vial types. In the case of large samples, it is advisable to carry out detection in vials filled up to their full capacity; in the case of small samples, the whole volume should be transferred to the vials and completed up to 9 ml for nylon vials,10 ml for glass vials and 11 to 14 ml for polyethilene vials. On the other hand, plastic vials showed higher detection efficiency than ones. As to background radiation, the lowest rates were given by nylon vials and the highest by Beckman glass vials [pt

  6. Development of a homogeneous pulse shape discriminating flow-cell radiation detection system

    International Nuclear Information System (INIS)

    Hastie, K.H.; DeVol, T.A.; Fjeld, R.A.


    A homogeneous flow-cell radiation detection system which utilizes coincidence counting and pulse shape discrimination circuitry was assembled and tested with five commercially available liquid scintillation cocktails. Two of the cocktails, Ultima Flo (Packard) and Mono Flow 5 (National Diagnostics) have low viscosities and are intended for flow applications; and three of the cocktails, Optiphase HiSafe 3 (Wallac), Ultima Gold AB (Packard), and Ready Safe (Beckman), have higher viscosities and are intended for static applications. The low viscosity cocktails were modified with 1-methylnaphthalene to increase their capability for alpha/beta pulse shape discrimination. The sample loading and pulse shape discriminator setting were optimized to give the lowest minimum detectable concentration for methylnaphthalenein a 30 s count time. Of the higher viscosity cocktails, Optiphase HiSafe 3 had the lowest minimum detectable activities for alpha and beta radiation, 0.2 and 0.4 Bq/ml for 233 U and 90 Sr/ 90 Y, respectively, for a 30 s count time. The sample loading was 70% and the corresponding alpha/beta spillover was 5.5%. Of the low viscosity cocktails, Mono Flow 5 modified with 2.5% (by volume) 1-methylnaphthalene resulted in the lowest minimum detectable activities for alpha and beta radiation; 0.3 and 0.5 Bq/ml for 233 U and 90 Sr/ 90 Y, respectively, for a 30 s count time. The sample loading was 50%, and the corresponding alpha/beta spillover was 16.6%. HiSafe 3 at a 10% sample loading was used to evaluate the system under simulated flow conditions

  7. Properties of human blood monocytes. I. CD91 expression and log orthogonal light scatter provide a robust method to identify monocytes that is more accurate than CD14 expression. (United States)

    Hudig, Dorothy; Hunter, Kenneth W; Diamond, W John; Redelman, Doug


    This study was designed to improve identification of human blood monocytes by using antibodies to molecules that occur consistently on all stages of monocyte development and differentiation. We examined blood samples from 200 healthy adults without clinically diagnosed immunological abnormalities by flow cytometry (FCM) with multiple combinations of antibodies and with a hematology analyzer (Beckman LH750). CD91 (α2 -macroglobulin receptor) was expressed only by monocytes and to a consistent level among subjects [mean median fluorescence intensity (MFI) = 16.2 ± 3.2]. Notably, only 85.7 ± 5.82% of the CD91(+) monocytes expressed high levels of the classical monocyte marker CD14, with some CD91(+) CD16(+) cells having negligible CD14, indicating that substantial FCM under-counts will occur when monocytes are identified by high CD14. CD33 (receptor for sialyl conjugates) was co-expressed with CD91 on monocytes but CD33 expression varied by nearly ten-fold among subjects (mean MFI = 17.4 ± 7.7). In comparison to FCM analyses, the hematology analyzer systematically over-counted monocytes and eosinophils while lymphocyte and neutrophil differential values generally agreed with FCM methods. CD91 is a better marker to identify monocytes than CD14 or CD33. Furthermore, FCM (with anti-CD91) identifies monocytes better than a currently used clinical CBC instrument. Use of anti-CD91 together with anti-CD14 and anti-CD16 supports the identification of the diagnostically significant monocyte populations with variable expression of CD14 and CD16. Copyright © 2013 Clinical Cytometry Society.

  8. First two-reagent vitamin D assay for general clinical chemistry. (United States)

    Saida, Fakhri B; Padilla-Chee, Mario; Dou, Chao; Yuan, Chong


    Vitamin D is a lipid-soluble molecule that plays key physiological roles in the metabolism of calcium, phosphate and magnesium. Recent studies show that deficiency in vitamin D is linked to cardiovascular diseases, autoimmune diseases and cancer. As a result, regular monitoring of 25-OH vitamin D (the main circulating form of vitamin D) is becoming essential. Current 25-OH vitamin D testing methodologies are cumbersome (too many reagents, long incubation times, phase separation) and are not compatible with general clinical chemistry platforms. Here, we report on a novel method to detect 25-OH vitamin D that is fast (results in 10 min or less), simple (two reagents) and compatible with virtually all general clinical chemistry analyzers. An immunoturbidimetric assay for 25-OH vitamin D (the Diazyme EZ Vitamin D Assay) has been developed using nanoparticles and vitamin D-specific antibodies. The performance of the assay kit, which consists of two reagents and five calibrators, was tested on the Beckman AU680 analyzer (AU680). The new assay was precise, sensitive (LOD = 7.2 nmol/L), linear (up to 390.1 nmol/L) and correlated strongly (R 2  > 0.95) with major commercial 25-OH vitamin D assays. Additionally, the assay was found to be the fastest to date, with the first results obtained within 10 min. Throughput on the AU680 was estimated at over 300 tests per hour. The newly developed 25-OH vitamin D assay is fast, precise and accurate. It can be run on most general chemistry analyzers. This assay aims at providing vitamin D-testing capabilities to all clinical chemistry laboratories. Copyright © 2018 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  9. Glucose meters: evaluation of the new formulation measuring strips from Roche (Accu-Chek) and Abbott (MediSense). (United States)

    Dimeski, G; Jones, B W; Tilley, V; Greenslade, M N; Russell, A W


    Both Roche and Abbott have released new glucose meter strips. They supply the entire Australian hospital market. The present study compared the performance of the new strips utilizing various specimen types (capillary, venous lithium heparin whole blood, venous lithium heparin plasma and serum) and evaluated how well they comply with the International Standards Organization (ISO) 15197 criteria. The study included imprecision, patient comparison and interference studies. Participants with and without diabetes were recruited to evaluate the performance of various specimen types against the Beckman DxC800 glucose method. The strips were tested for different interferences: galactose, maltose, lactose, Icodextrin, Intragam, paracetamol, sodium, ascorbic acid, variable strip storage temperature, haematocrit, haemolysis and lipaemia. The imprecision of the two strips was approximately 5% or less, except for the Abbott strip at very low values (1.4 mmol/L), approximately 7%. In total, 78% and 84%, respectively, of the results from the finger prick capillary specimens with the Roche (Accu-Chek Performa meter) and Abbott (Optium Xceed meter) strips, not 95% or greater as recommended by the ISO guideline, were within the recommended limits compared with reference plasma estimation on laboratory analysers. Galactose, ascorbic acid, haematocrit and sodium on the Roche and ascorbic acid and haematocrit on the Abbott strip continue to interfere to a variable degree with the glucose measurement. Analytically small differences exist between the glucose meter strips. The most significant analytical difference with the strips was at low glucose levels when compared with laboratory analyses and this may be of clinical importance. The impact of some of the interferences is variable between the two strips. Individuals, health-care professionals and health-care institutions should consider these data when selecting glucose meters for the management of people with diabetes mellitus.

  10. Transference of CALIPER pediatric reference intervals to biochemical assays on the Roche cobas 6000 and the Roche Modular P. (United States)

    Higgins, Victoria; Chan, Man Khun; Nieuwesteeg, Michelle; Hoffman, Barry R; Bromberg, Irvin L; Gornall, Doug; Randell, Edward; Adeli, Khosrow


    The Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) has recently established pediatric age- and sex-specific reference intervals for over 85 biochemical markers on the Abbott Architect system. Previously, CALIPER reference intervals for several biochemical markers were successfully transferred from Abbott assays to Roche, Beckman, Ortho, and Siemens assays. This study further broadens the CALIPER database by performing transference and verification for 52 biochemical assays on the Roche cobas 6000 and the Roche Modular P. Using CLSI C28-A3 and EP9-A2 guidelines, transference of the CALIPER reference intervals was attempted for 16 assays on the Roche cobas 6000 and 36 on the Modular P. Calculated reference intervals were further verified using 100 healthy CALIPER samples. Most assays showed strong correlation between assay systems and were transferable from Abbott to the Roche cobas 6000 (81%) and the Modular P (86%). Bicarbonate and magnesium were not transferable on either system and calcium and prealbumin were not transferable to the Modular P. Of the transferable analytes, 62% and 61% were verified on the cobas 6000 and the Modular P, respectively. This study extends the utility of the CALIPER database to two additional analytical systems, which facilitates the broad application of CALIPER reference intervals at pediatric centers utilizing Roche biochemical assays. Transference studies across different analytical platforms can later be collectively analyzed in an attempt to develop common reference intervals across all clinical chemistry instruments to harmonize laboratory test interpretation in diagnosis and monitoring of pediatric disease. Copyright © 2015 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  11. Evaluation of the serum zinc level in erosive and non-erosive oral lichen planus. (United States)

    Gholizadeh, N; Mehdipour, M; Najafi, Sh; Bahramian, A; Garjani, Sh; Khoeini Poorfar, H


    Lichen planus is a chronic inflammatory immunologic-based disease involving skin and mucosa. This disease is generally divided into two categories: erosive and non-erosive. Many etiologic factors are deliberated regarding the disease; however, the disorders of immune system and the role of cytotoxic T-lymphocytes and monocytes are more highlighted. Zinc is an imperative element for the growth of epithelium and its deficiency induces the cytotoxic activity of T-helper2 cells, which seems to be associated with lichen planus. This study was aimed to evaluate the levels of serum zinc in erosive and non-erosive oral lichen planus (OLP) and to compare it with the healthy control group to find out any feasible inference. A total of 22 patients with erosive oral lichen planus, 22 patients with non erosive OLP and 44 healthy individuals as the control group were recruited in this descriptive-comparative study. All the participants were selected from the referees to the department of oral medicine, school of dentistry, Tabriz University of Medical Sciences. Serum zinc level was examined for all the individuals with liquid-stat kit (Beckman Instruments Inc.; Carlsbad, CA). Data were analyzed by adopting the ANOVA and Tukey tests, using SPSS 16 statistical software. The mean age of patients with erosive and non-erosive LP was 41.7 and 41.3 years, respectively. The mean age of the healthy control group was 34.4 years .The mean serum zinc levels in the erosive and non erosive lichen planus groups and control groups were 8.3 (1.15), 11.15 (0.92) and 15.74 (1.75) μg/dl respectively. The difference was statistically significant (poral lichen planus. This finding may probably indicate the promising role of zinc in development of oral lichen planus.

  12. Development of the TeamOBS-PPH - targeting clinical performance in postpartum hemorrhage. (United States)

    Brogaard, Lise; Hvidman, Lone; Hinshaw, Kim; Kierkegaard, Ole; Manser, Tanja; Musaeus, Peter; Arafeh, Julie; Daniels, Kay I; Judy, Amy E; Uldbjerg, Niels


    This study aimed to develop a valid and reliable TeamOBS-PPH tool for assessing clinical performance in the management of postpartum hemorrhage (PPH). The tool was evaluated using video-recordings of teams managing PPH in both real-life and simulated settings. A Delphi panel consisting of 12 obstetricians from the UK, Norway, Sweden, Iceland, and Denmark achieved consensus on (i) the elements to include in the assessment tool, (ii) the weighting of each element, and (iii) the final tool. The validity and reliability were evaluated according to Cook and Beckman. (Level 1) Four raters scored four video-recordings of in situ simulations of PPH. (Level 2) Two raters scored 85 video-recordings of real-life teams managing patients with PPH ≥1000 mL in two Danish hospitals. (Level 3) Two raters scored 15 video-recordings of in situ simulations of PPH from a US hospital. The tool was designed with scores from 0 to 100. (Level 1) Teams of novices had a median score of 54 (95% CI 48-60), whereas experienced teams had a median score of 75 (95% CI 71-79; p < 0.001). (Level 2) The intra-rater [intra-class correlation (ICC) = 0.96] and inter-rater (ICC = 0.83) agreements for real-life PPH were strong. The tool was applicable in all cases: atony, retained placenta, and lacerations. (Level 3) The tool was easily adapted to in situ simulation settings in the USA (ICC = 0.86). The TeamOBS-PPH tool appears to be valid and reliable for assessing clinical performance in real-life and simulated settings. The tool will be shared as the free TeamOBS App. © 2018 Nordic Federation of Societies of Obstetrics and Gynecology.

  13. Screening for fetal chromosome abnormalities during the second trimester

    International Nuclear Information System (INIS)

    Dong Hui; Li Ming; Li Ping


    Objective: To develop a pre -natal screening program for fetal chromosome abnormalities based on risk values calculated from maternal serum markers levels during the second trimester. Methods: Serum levels of AFP, β-HCG, uE 3 were determined with CLIA in 1048 pregnant women during 14-21w gestation period and the results were analyzed with a specific software (screening program for Down' s syndrome developed by Beckman) for the risk rate. In those women defined as being of high risk rate, cells from amniotic fluid or umbilical cord blood were studied for karyotype analysis. Results: Of these 1048 women, 77 were designated as being of high risk rate for several chromosome abnormalities i.e. Down's syndrome, open spina bifida and trisomy -18 syndrome (overall positive rate 7.3%). Further fetal chromosome study in 31 of them revealed three proven cases of abnormality. Another cord blood study was performed in a calculated low risk rate case but with abnormal sonographic finding at 31 w gestation and proved to be abnormal (software study false negative). The remaining 46 high risk rate cases either refused future study (n=35) or were lost for follow-up (n=11). Fortunately, all the 35 women refused further study gave birth to normal babies without any chromosome abnormalities discovered on peripheral blood study. Besides, in a trial study, five high risk rate women were again evaluated a few weeks later but with tremendous difference between the results. Conclusion: The present program proves to be clinically useful but needs further study and revision. Many factors may influence the result of the analysis and the duration of gestation period in weeks should be as accurate as possible. At present, in order to avoid getting false negatives, we don't advise a second check in 'high risk' cases. (authors)

  14. Guidelines and Recommendations for Laboratory Analysis in the Diagnosis and Management of Diabetes Mellitus (United States)

    Arnold, Mark; Bakris, George L.; Bruns, David E.; Horvath, Andrea Rita; Kirkman, M. Sue; Lernmark, Ake; Metzger, Boyd E.; Nathan, David M.


    BACKGROUND Multiple laboratory tests are used to diagnose and manage patients with diabetes mellitus. The quality of the scientific evidence supporting the use of these tests varies substantially. APPROACH An expert committee compiled evidence-based recommendations for the use of laboratory testing for patients with diabetes. A new system was developed to grade the overall quality of the evidence and the strength of the recommendations. Draft guidelines were posted on the Internet and presented at the 2007 Arnold O. Beckman Conference. The document was modified in response to oral and written comments, and a revised draft was posted in 2010 and again modified in response to written comments. The National Academy of Clinical Biochemistry and the Evidence-Based Laboratory Medicine Committee of the American Association for Clinical Chemistry jointly reviewed the guidelines, which were accepted after revisions by the Professional Practice Committee and subsequently approved by the Executive Committee of the American Diabetes Association. CONTENT In addition to long-standing criteria based on measurement of plasma glucose, diabetes can be diagnosed by demonstrating increased blood hemoglobin A1c (HbA1c) concentrations. Monitoring of glycemic control is performed by self-monitoring of plasma or blood glucose with meters and by laboratory analysis of HbA1c. The potential roles of noninvasive glucose monitoring, genetic testing, and measurement of autoantibodies, urine albumin, insulin, proinsulin, C-peptide, and other analytes are addressed. SUMMARY The guidelines provide specific recommendations that are based on published data or derived from expert consensus. Several analytes have minimal clinical value at present, and their measurement is not recommended. PMID:21617108

  15. Assessing composition and structure of soft biphasic media from Kelvin-Voigt fractional derivative model parameters (United States)

    Zhang, Hongmei; Wang, Yue; Fatemi, Mostafa; Insana, Michael F.


    Kelvin-Voigt fractional derivative (KVFD) model parameters have been used to describe viscoelastic properties of soft tissues. However, translating model parameters into a concise set of intrinsic mechanical properties related to tissue composition and structure remains challenging. This paper begins by exploring these relationships using a biphasic emulsion materials with known composition. Mechanical properties are measured by analyzing data from two indentation techniques—ramp-stress relaxation and load-unload hysteresis tests. Material composition is predictably correlated with viscoelastic model parameters. Model parameters estimated from the tests reveal that elastic modulus E 0 closely approximates the shear modulus for pure gelatin. Fractional-order parameter α and time constant τ vary monotonically with the volume fraction of the material’s fluid component. α characterizes medium fluidity and the rate of energy dissipation, and τ is a viscous time constant. Numerical simulations suggest that the viscous coefficient η is proportional to the energy lost during quasi-static force-displacement cycles, E A . The slope of E A versus η is determined by α and the applied indentation ramp time T r. Experimental measurements from phantom and ex vivo liver data show close agreement with theoretical predictions of the η -{{E}A} relation. The relative error is less than 20% for emulsions 22% for liver. We find that KVFD model parameters form a concise features space for biphasic medium characterization that described time-varying mechanical properties. The experimental work was carried out at the Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Methodological development, including numerical simulation and all data analysis, were carried out at the school of Life Science and Technology, Xi’an JiaoTong University, 710049, China.

  16. Commutability of possible external quality assessment materials for cardiac troponin measurement.

    Directory of Open Access Journals (Sweden)

    Shunli Zhang

    Full Text Available The measurement of cardiac troponin is crucial in the diagnosis of myocardial infarction. The performance of troponin measurement is most conveniently monitored by external quality assessment (EQA programs. The commutability of EQA samples is often unknown and the effectiveness of EQA programs is limited.Commutability of possible EQA materials was evaluated. Commercial control materials used in an EQA program, human serum pools prepared from patient samples, purified analyte preparations, swine sera from model animals and a set of patient samples were measured for cTnI with 4 assays including Abbott Architect, Beckman Access, Ortho Vitros and Siemens Centaur. The measurement results were logarithm-transformed, and the transformed data for patient samples were pairwise analyzed with Deming regression and 95% prediction intervals were calculated for each pair of assays. The commutability of the materials was evaluated by comparing the logarithmic results of the materials with the limits of the intervals. Matrix-related biases were estimated for noncommutable materials. The impact of matrix-related bias on EQA was analyzed and a possible correction for the bias was proposed.Human serum pools were commutable for all assays; purified analyte preparations were commutable for 2 of the 6 assay pairs; commercial control materials and swine sera were all noncommutable; swine sera showed no reactivity to Vitros assay. The matrix-related biases for noncommutable materials ranged from -83% to 944%. Matrix-related biases of the EQA materials caused major abnormal between-assay variations in the EQA program and correction of the biases normalized the variations.Commutability of materials has major impact on the effectiveness of EQA programs for cTnI measurement. Human serum pools prepared from patient samples are commutable and other materials are mostly noncommutable. EQA programs should include at least one human serum pool to allow proper interpretation of

  17. Precision and accuracy of blood glucose measurements using three different instruments. (United States)

    Nowotny, B; Nowotny, P J; Strassburger, K; Roden, M


    Assessment of insulin sensitivity by dynamic metabolic tests such as the hyperinsulinemic euglycemic clamp critically relies on the reproducible and fast measurement of blood glucose concentrations. Although various instruments have been developed over the last decades, little is known as to the accuracy and comparability. We therefore compared the best new instrument with the former gold standard instruments to measure glucose concentrations in metabolic tests. Fasting blood samples of 15 diabetic and 10 healthy subjects were collected into sodium-fluoride tubes, spiked with glucose (0, 2.8, 6.9 and 11.1 mmol/l) and measured either as whole blood (range 3.3-26.3 mmol/l) or following centrifugation as plasma (range 3.9-32.0 mmol/l). Plasma samples were analyzed in the YSI-2300 STAT plus (YSI), EKF Biosen C-Line (EKF) and the reference method, Beckman Glucose analyzer-II (BMG), whole blood samples in EKF instruments with YSI as reference method. The average deviation of the EKF from the reference, BMG, was 3.0 ± 3.5% without any concentration-dependent variability. Glucose measurements by YSI were in good agreement with that by BMG (plasma) and EKF (plasma and whole blood) up to concentrations of 13.13 mmol/l (0.5 ± 3.7%), but deviation increased to -6.2 ± 3.8% at higher concentrations. Precision (n = 6) was ±2.2% (YSI), ±3.9% (EKF) and ±5.2% (BMG). The EKF instrument is comparable regarding accuracy and precision to the reference method BMG and can be used in metabolic tests, while the YSI showed a systematic shift at higher glucose concentrations. Based on these results we decided to replace BMG with EKF instrument in metabolic tests. © 2012 The Authors. Diabetic Medicine © 2012 Diabetes UK.

  18. Annual symposium on Frontiers in Science

    Energy Technology Data Exchange (ETDEWEB)

    Metzger, N.; Fulton, K.R.


    This final report summarizes activities conducted for the National Academy of Sciences' Annual Symposium on Frontiers of Science with support from the US Department of Energy for the period July 1, 1993 through May 31, 1998. During the report period, five Frontiers of Science symposia were held at the Arnold and Mabel Beckman Center of the National Academies of Sciences and Engineering. For each Symposium, an organizing committee appointed by the NAS President selected and planned the eight sessions for the Symposium and identified general participants for invitation by the NAS President. These Symposia accomplished their goal of bringing together outstanding younger (age 45 or less) scientists to hear presentations in disciplines outside their own and to discuss exciting advances and opportunities in their fields in a format that encourages, and allows adequate time for, informal one-on-one discussions among participants. Of the 458 younger scientists who participated, over a quarter (124) were women. Participant lists for all symposia (1993--1997) are attached. The scientific participants were leaders in basic research from academic, industrial, and federal laboratories in such disciplines as astronomy, astrophysics, atmospheric science, biochemistry, cell biology, chemistry, computer science, earth sciences, engineering, genetics, material sciences, mathematics, microbiology, neuroscience, physics, and physiology. For each symposia, the 24 speakers and discussants on the program were urged to focus their presentations on current cutting-edge research in their field for a scientifically sophisticated but non-specialist audience, and to provide a sense of the experimental data--what is actually measured and seen in the various fields. They were also asked to address questions such as: What are the major research problems and unique tools in their field? What are the current limitations on advances as well as the frontiers? Speakers were asked to provide a

  19. The Putative Role of the Non-Gastric H+/K+-ATPase ATP12A (ATP1AL1 as Anti-Apoptotic Ion Transporter: Effect of the H+/K+ ATPase Inhibitor SCH28080 on Butyrate-Stimulated Myelomonocytic HL-60 Cells

    Directory of Open Access Journals (Sweden)

    Martin Jakab


    Full Text Available Background/Aims: The ATP12A gene codes for a non-gastric H+/K+ ATPase, which is expressed in a wide variety of tissues. The aim of this study was to test for the molecular and functional expression of the non-gastric H+/K+ ATPase ATP12A/ATP1AL1 in unstimulated and butyrate-stimulated (1 and 10 mM human myelomonocytic HL-60 cells, to unravel its potential role as putative apoptosis-counteracting ion transporter as well as to test for the effect of the H+/K+ ATPase inhibitor SCH28080 in apoptosis. Methods: Real-time reverse-transcription PCR (qRT-PCR was used for amplification and cloning of ATP12A transcripts and to assess transcriptional regulation. BCECF microfluorimetry was used to assess changes of intracellular pH (pHi after acute intracellular acid load (NH4Cl prepulsing. Mean cell volumes (MCV and MCV-recovery after osmotic cell shrinkage (Regulatory Volume Increase, RVI were assessed by Coulter counting. Flow-cytometry was used to measure MCV (Coulter principle, to assess apoptosis (phosphatidylserine exposure to the outer leaflet of the cell membrane, caspase activity, 7AAD staining and differentiation (CD86 expression. Results: We found by RT-PCR, intracellular pH measurements, MCV measurements and flow cytometry that ATP12A is expressed in human myelomonocytic HL-60 cells. Treatment of HL-60 cells with 1 mM butyrate leads to monocyte-directed differentiation whereas higher concentrations (10 mM induce apoptosis as assessed by flow-cytometric determination of CD86 expression, caspase activity, phosphatidylserine exposure on the outer leaflet of the cell membrane and MCV measurements. Transcriptional up-regulation of ATP12A and CD86 is evident in 1 mM butyrate-treated HL-60 cells. The H+/K+ ATPase inhibitor SCH28080 (100 µM diminishes K+-dependent pHi recovery after intracellular acid load and blocks RVI after osmotic cell shrinkage. After seeding, HL-60 cells increase their MCV within the first 24 h in culture, and subsequently

  20. Sources of SOA gaseous precursors in contrasted urban environments: a focus on mono-aromatic compounds and intermediate volatility compounds (United States)

    Salameh, Therese; Borbon, Agnès; Ait-Helal, Warda; Afif, Charbel; Sauvage, Stéphane; Locoge, Nadine; Bonneau, Stéphane; Sanchez, Olivier


    , SP95 E10, and SP98) and was used to constraint evaporative emissions in order to predict the headspace vapour composition (Harley and Coulter-Burke, 2000). Modelled and observed compositions are in good agreement (differences up to 20%). Therefore, the implemented model is a relevant tool to test the sensitivity of BTEX and other VOCs ambient composition to evaporative emissions of fuels with regards to their composition. Such analysis will be extended to other target cities and similarities/differences will be presented regarding regional characteristics. This work was supported by the Ile de France region, Life and PHOTOPAQ grant, PICS-CNRS, ENVIMED and ChArMEx. We would like to thank Laurence Dépelchin and Thierry Léonardis for technical support and AIRPARIF for providing the data. Borbon, A., et al. (2013) Emission ratios of anthropogenic VOC in northern mid-latitude megacities: observations vs. emission inventories in Los Angeles and Paris, J. Geophys. Res. 118, 2041 - 2057. Harley, R. and Coulter-Burke, S. (2000) Relating Liquid Fuel and Headspace Vapor Composition for California Reformulated Gasoline Samples Containing Ethanol, Environ. Sci. Technol. 34, 4088-4094. Ait-Helal, W.; Borbon, A.; Sauvage, S.; et al., Atmos. Chem. Phys. vol. 14 , No. 19 , p. 10439-10464

  1. Single Cell Electroporation Method for Mammalian CNS Neurons in Organotypic Slice Cultures (United States)

    Uesaka, Naofumi; Hayano, Yasufumi; Yamada, Akito; Yamamoto, Nobuhiko

    Axon tracing is an essential technique to study the projection pattern of neurons in the CNS. Horse radish peroxidase and lectins have contributed to revealing many neural connection patterns in the CNS (Itaya and van Hoesen, 1982; Fabian and Coulter, 1985; Yoshihara, 2002). Moreover, a tracing method with fluorescent dye has enabled the observation of growing axons in living conditions, and demon strated a lot of developmental aspects in axon growth and guidance (Harris et al., 1987; O'Rourke and Fraser, 1990; Kaethner and Stuermer, 1992; Halloran and Kalil, 1994; Yamamoto et al., 1997). More recently, genetically encoded fluores cent proteins can be used as a powerful tool to observe various biological events. Several gene transfer techniques such as microinjection, biolistic gene gun, viral infection, lipofection and transgenic technology have been developed (Feng et al., 2000; Ehrengruber et al., 2001; O'Brien et al., 2001; Ma et al., 2002; Sahly et al., 2003). In particular, the electroporation technique was proved as a valuable tool, since it can be applied to a wide range of tissues and cell types with little toxicity and can be performed with relative technical easiness. Most methods, including a stand ard electroporation technique, are suitable for gene transfer to a large number of cells. However, this is not ideal for axonal tracing, because observation of individ ual axons is occasionally required. To overcome this problem, we have developed an electroporation method using glass micropipettes containing plasmid solutions and small current injection. Here we introduce the method in detail and exemplified results with some example applications and discuss its usefulness.

  2. Dietary branched-chain amino acid (BCAA) and tumor growth

    Energy Technology Data Exchange (ETDEWEB)

    Chan, W.; Baron, L.; Baron, P.; White, F.; Banks, W.L. Jr.


    The effects of high dietary BCAA on tumor growth was examined in adult male Fischer 344 rats inoculated with 10/sup 6/ viable MCA fibrosarcoma cells. Ten days after tumor inoculation, when tumors were of palpable size, rats were divided into two groups at random. The experimental(E) group was fed the AIN-76 diet supplemented with 4X the BCAA content of diet casein and the control(C) group was fed the AIN-76 made isonitrogenous with the E diet by glutamic acid supplementation. Five rats from each group were killed at days 0,3,6, and 9. Rats were injected with /sup 14/C-Tyrosine and /sup 3/H-Thymidine i.p. (2 and 4 uCi/100g BW, respectively) an hour before they were killed. The incorporation of /sup 14/C and /sup 3/H into the acid insoluble fraction of the tumor tissues samples were measured. Single cell suspension of tumor were prepared for cell cycle kinetics analysis using a Coulter EPICS IV flow microflorometer. The percentage of normal and hyperdiploid cells were analyzed. Results showed that both tumor size and weight were doubled at each time point the rats were killed. At day 0, the /sup 3/H and /sup 14/C incorporation were 32 +/- 10dpm and 27 +/- 4dpm/mg tumor, respectively. The /sup 3/H incorporation dropped in both diet groups at days 6 and 9 but the /sup 14/C incorporation showed a decrease only at day 9. These changes were statistically significant, P>0.05. No difference in the tumor growth parameters used in this study can be attributed to the high dietary BCAA.

  3. Dietary branched-chain amino acid (BCAA) and tumor growth

    International Nuclear Information System (INIS)

    Chan, W.; Baron, L.; Baron, P.; White, F.; Banks, W.L. Jr.


    The effects of high dietary BCAA on tumor growth was examined in adult male Fischer 344 rats inoculated with 10 6 viable MCA fibrosarcoma cells. Ten days after tumor inoculation, when tumors were of palpable size, rats were divided into two groups at random. The experimental(E) group was fed the AIN-76 diet supplemented with 4X the BCAA content of diet casein and the control(C) group was fed the AIN-76 made isonitrogenous with the E diet by glutamic acid supplementation. Five rats from each group were killed at days 0,3,6, and 9. Rats were injected with 14 C-Tyrosine and 3 H-Thymidine i.p. (2 and 4 uCi/100g BW, respectively) an hour before they were killed. The incorporation of 14 C and 3 H into the acid insoluble fraction of the tumor tissues samples were measured. Single cell suspension of tumor were prepared for cell cycle kinetics analysis using a Coulter EPICS IV flow microflorometer. The percentage of normal and hyperdiploid cells were analyzed. Results showed that both tumor size and weight were doubled at each time point the rats were killed. At day 0, the 3 H and 14 C incorporation were 32 +/- 10dpm and 27 +/- 4dpm/mg tumor, respectively. The 3 H incorporation dropped in both diet groups at days 6 and 9 but the 14 C incorporation showed a decrease only at day 9. These changes were statistically significant, P>0.05. No difference in the tumor growth parameters used in this study can be attributed to the high dietary BCAA

  4. Macronutrients leaf contents of corn in intercropping with forages of genus Panicum and Urochloa in simultaneous seeding

    Directory of Open Access Journals (Sweden)

    Denise Tsuzukibashi


    Full Text Available The planting of crops in degraded pasture areas is a formula used for decades by farmers to recover the productive capacity of pastures and soils. The integrated crop-livestock (ICL consists of different production systems of grains, fibers, wood, meat, milk and agro-deployed in the same area, in intercrop, rotation or succession. Typically this integration mainly involves the planting of grain and pasture in the recovery or deployment. This work aimed to evaluate the macronutrients leaf contents of irrigated corn intercropped with forages of the genus Panicum and Urochloa simultaneously to sown corn. The experiment was conducted at the Farm for Teaching, Research and Extension, Faculty of Engineering - UNESP, Ilha Solteira in an Oxisol in Savannah conditions, in experimental area that had a history of no-tillage to 8 years (previous crop corn. The experimental design used was randomized blocks with four replications and five treatments: Panicum maximum cv. Tanzania sown simultaneously (CTS corn; Panicum maximum cv. Mombaça sown simultaneously (CMS to corn; Urochloa brizantha cv. Xaraes sown simultaneously (CBS corn; Urochloa ruziziensis sown simultaneously (CRS to corn, and corn without intercropping (CWI. The seeds of grasses were sown in spacing of 0.34 m, being sown with a seed drill with disc coulters mounted mechanism for no-tillage system at a depth of 0.03 m. There was no significant difference between the single corn tillage and intercropping with different modalities of forage genus Panicum and Urochloa to the leaf contents of N, P, K, Ca and Mg, demonstrating the non-compete forages with corn in a intercrop on the absorption of these nutrients. In respect to S, CTS presented higher content of S foliar when compared to CWI (Table 1. The absorption of nutrients by corn are not affect by the intercrop with forages of the genus Panicum and Urochloa, in simultaneously sown.

  5. Effect of caffeine on gamma-ray induced G2 arrest in well-synchronized Chinese hamster ovary cells in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Masunaga, Shin-ichiro [Kyoto Univ., Kumatori, Osaka (Japan). Research Reactor Inst.; Keng, P.C.


    G1-rich cells were separated from exponentially growing asynchronous cultured Chinese hamster ovary (CHO-K1) cells by centrifugal elutriation and a Coulter Counter. The G1-rich cells were incubated in medium that contained hydroxyurea (HU) to kill S phase cells and obtain the purest G1/S boundary cells possible. The HU-treated cells were washed, and were again incubated, in medium without HU, to allow these well-synchronized G1/S boundary cells to progress to S and G2/M phases. At various times after release from G1/S boundary, 4 Gy of gamma-ray and/or caffeine was administered to the cells. Eight hours after the removal of HU, cell-cycle analysis was performed with a flow cytometer. G2 arrest induced by gamma-rays was clearly shown when radiation was given earlier than 6.5 hours after HU removal. G2 arrest induced by radiation given 0.5-6.5 hours after HU removal was reduced by caffeine treatment given 6.0-6.5 hours after HU removal. Caffeine released radiation-induced G2 arrest when the radiation was given before the cultured cells entered G2/M phase and when caffeine was applied to the irradiated cells at the time when G1/S boundary cells not treated by radiation or with caffeine entered G2/M phase. Our method of centrifugal elutriation combined with incubation with HU was useful for isolating pure G1/S boundary cells from in vitro asynchronous cultures. (author)

  6. Formation of microscopic particles during the degradation of different polymers. (United States)

    Lambert, Scott; Wagner, Martin


    This study investigated the formation and size distribution of microscopic plastic particles during the degradation of different plastic materials. Particle number concentrations in the size range 30 nm-60 μm were measured by nanoparticle tracking analysis (NTA) and Coulter Counter techniques. Each of the plastics used exhibited a measureable increase in the release of particles into the surrounding solution, with polystyrene (PS) and polylactic acid (PLA) generating the highest particle concentrations. After 112 d, particle concentrations ranged from 2147 particles ml(-1) in the control (C) to 92,465 particles ml(-1) for PS in the 2-60 μm size class; 1.2 × 10(5) particles ml(-1) (C) to 11.6 × 10(6) for PLA in the 0.6-18 μm size class; and 0.2 × 10(8) particles ml(-1) (C) to 6.4 × 10(8) particles ml(-1) for PS in the 30-2000 nm size class (84 d). A classification of samples based on principal component analysis showed a separation between the different plastic types, with PLA clustering individually in each of the three size classes. In addition, particle size distribution models were used to examine more closely the size distribution data generated by NTA. Overall, the results indicate that at the beginning of plastic weathering processes chain scission at the polymer surface causes many very small particles to be released into the surrounding solution and those concentrations may vary between plastic types. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Eosinophils, pruritus and psoriasis: effects of treatment with etretinate or cyclosporin-A. (United States)

    Schopf, R E; Hultsch, T; Lotz, J; Bräutigam, M


    The antipsoriatic drugs cyclosporin A (CyA) and etretinate have been found to influence proinflammatory eosinophilic leukocytes and pruritus. We compared the number of blood eosinophils, concentration of serum eosinophil cationic protein (ECP), and pruritus in patients with psoriasis treated with either CyA or etretinate. Patients with psoriasis vulgaris were randomly assigned to treatment for 10 weeks with either CyA (n = 21) or etretinate (n = 10). The psoriasis area-and-severity index (PASI-score) and pruritus (according to a 0-3 scale) served as clinical parameters, the blood esosinophil counts (Coulter Counter) and the serum ECP (RIA, Pharmacia) as laboratory parameters. After CyA treatment the PASI-score amounted to 24 +/- 4%, after etretinate to 56 +/- 6% of the initial values (mean +/- SEM). One week after CyA treatment, esosinophils dropped from 190 +/- 21 to 137 +/- 16/microliter (P = 0.038, Wilcoxon test), after 10 weeks to 127 +/- 18/microliter (P = 0.006). By contrast, under etretinate blood eosinophil counts only changed marginally. Before treatment, ECP concentrations of 15.71 +/- 1.30 (CyA) and 15.3 +/- 5.53 micrograms/l (etretinate) were measured (normal range 3-16 micrograms/l), ECP remained constant under both CyA and etretinate or tended to increase after 10 weeks; about 50% of the patients exhibited elevated ECP concentrations. Pruritus diminished more with CyA than etretinate therapy. PASI-scores and pruritus were directly proportional. We conclude that treatment of psoriasis with CyA leads to a rapid drop of blood eosinophils and that the activation state of eosinophils does not decrease after antipsoriatic treatment. Pruritus in psoriasis is coupled to disease severity. The underlying antipsoriatic mechanisms of CyA may be linked to lowering the number of blood eosinophils.

  8. Technical note: Alternatives to reduce adipose tissue sampling bias. (United States)

    Cruz, G D; Wang, Y; Fadel, J G


    Understanding the mechanisms by which nutritional and pharmaceutical factors can manipulate adipose tissue growth and development in production animals has direct and indirect effects in the profitability of an enterprise. Adipocyte cellularity (number and size) is a key biological response that is commonly measured in animal science research. The variability and sampling of adipocyte cellularity within a muscle has been addressed in previous studies, but no attempt to critically investigate these issues has been proposed in the literature. The present study evaluated 2 sampling techniques (random and systematic) in an attempt to minimize sampling bias and to determine the minimum number of samples from 1 to 15 needed to represent the overall adipose tissue in the muscle. Both sampling procedures were applied on adipose tissue samples dissected from 30 longissimus muscles from cattle finished either on grass or grain. Briefly, adipose tissue samples were fixed with osmium tetroxide, and size and number of adipocytes were determined by a Coulter Counter. These results were then fit in a finite mixture model to obtain distribution parameters of each sample. To evaluate the benefits of increasing number of samples and the advantage of the new sampling technique, the concept of acceptance ratio was used; simply stated, the higher the acceptance ratio, the better the representation of the overall population. As expected, a great improvement on the estimation of the overall adipocyte cellularity parameters was observed using both sampling techniques when sample size number increased from 1 to 15 samples, considering both techniques' acceptance ratio increased from approximately 3 to 25%. When comparing sampling techniques, the systematic procedure slightly improved parameters estimation. The results suggest that more detailed research using other sampling techniques may provide better estimates for minimum sampling.

  9. Grazing and metabolism of Euphausia pacifica in the Yellow Sea.

    Directory of Open Access Journals (Sweden)

    Zhencheng Tao

    Full Text Available Grazing and metabolism of Euphausia pacifica in the Yellow Sea were studied from September 2006 to August 2007. Euphausia pacifica is a selective-feeding omnivore and grazing rates among different months were monitored using a Coulter Counter and batch culture feeding experiments. Euphausia pacifica mainly grazed microzooplankton in August and September, which resulted in an increase in chlorophyll a concentration. Oxygen consumption rate of E. pacifica was 38.7-42.5 μmol O2 g(-1 DW h(-1 in March, which was four times higher than the oxygen consumption rates in September and December. The vigorous metabolism of E. pacifica in March consumed 3.1% of body carbon daily, which is likely related to its high reproduction and grazing rate. Respiration and metabolism of E. pacifica in September and December were similar and were lower. O:N ratio of E. pacifica was the highest (17.3-23.8 in March when spawning activity occurred and when food was abundant. The energetic source of E. pacifica during September and December was mostly protein from eating a carnivorous diet, including such items as microzooplankton. Euphausia pacifica was found in cold water at the bottom of the Yellow Sea in summer and autumn and maintained a low consumption status. O:N ratios of E. pacifica in March, September, and December were negatively correlated with SSTs and no significant correlation was found between O:N ratios and chlorophyll a concentration. Seawater temperature is clearly the most important parameter influencing the metabolism of E. pacifica.

  10. Climate contributes to zonal forest mortality in Southern California's San Jacinto Mountains (United States)

    Fellows, A.; Goulden, M.


    An estimated 4.6 million trees died over ~375,000 acres of Southern California forest in 2002-2004. This mortality punctuated a decline in forest health that has been attributed to air pollution, stem densification, or drought. Bark beetles were the proximate cause of most tree death but the underlying cause of this extensive mortality is arguably poor forest health. We investigated the contributions that climate, particularly drought, played in tree mortality and how physiological drought stress may have structured the observed patterns of mortality. Field surveys showed that conifer mortality was zonal in the San Jacinto Mountains of Southern California. The proportion of conifer mortality increased with decreasing elevation (p=0.01). Mid-elevation conifers (White Fir, Incense Cedar, Coulter Pine, Sugar Pine, Ponderosa and Jeffrey Pine) died in the lower portions of their respective ranges, which resulted in an upslope lean in species’ distribution and an upslope shift in species’ mean elevation. Long-term precipitation (P) is consistent with elevation over the conifer elevation range (p=0.43). Potential evapotranspiration (ET) estimated by Penman Monteith declines with elevation by nearly half over the same range. These trends suggest that ET, more than P, is critical in structuring the elevational trend in drought stress and may have contributed to the patterns of mortality that occurred in 2002-04. Physiological measurements in a mild drought year (2009) showed late summer declines in plant water availability with decreasing elevation (p < 0.01) and concomitant reductions in carbon assimilation and stomatal conductance with decreasing elevation. We tie these observations together with a simple water balance model.

  11. Differences in otosclerotic and normal human stapedial osteoblast properties are normalized by alendronate in vitro. (United States)

    Gronowicz, Gloria; Richardson, Yvonne L; Flynn, John; Kveton, John; Eisen, Marc; Leonard, Gerald; Aronow, Michael; Rodner, Craig; Parham, Kourosh


    Identify and compare phenotypic properties of osteoblasts from patients with otosclerosis (OSO), normal bones (HOB), and normal stapes (NSO) to determine a possible cause for OSO hypermineralization and assess any effects of the bisphosphonate, alendronate. OSO (n = 11), NSO (n = 4), and HOB (n = 13) cultures were assayed for proliferation, adhesion, mineralization, and gene expression with and without 10(-10)M-10(-8)M alendronate. Academic hospital. Cultures were matched for age, sex, and passage number. Cell attachment and proliferation + alendronate were determined by Coulter counting cells and assaying tritiated thymidine uptake, respectively. At 7, 14, and 21 days of culture + alendronate, calcium content and gene expression by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) were determined. OSO had significantly more cells adhere but less proliferation than NSO or HOB. Calcification was significantly increased in OSO compared to HOB and NSO. NSO and HOB had similar cell adhesion and proliferation rates. A dose-dependent effect of alendronate on OSO adhesion, proliferation, and mineralization was found, resulting in levels equal to NSO and HOB. All cultures expressed osteoblast-specific genes such as RUNX2, alkaline phosphatase, type I collagen, and osteocalcin. However, osteopontin was dramatically reduced, 9.4-fold at 14 days, in OSO compared to NSO. Receptor activator of nuclear factor κB ligand/osteoprotegerin (RANKL/OPG), important in bone resorption, was elevated in OSO with decreased levels of OPG levels. Alendronate had little effect on gene expression in HOB but in OSO increased osteopontin levels and decreased RANKL/OPG. OSO cultures displayed properties of hypermineralization due to decreased osteopontin (OPN) and also had increased RANKL/OPG, which were normalized by alendronate. © American Academy of Otolaryngology—Head and Neck Surgery Foundation 2014.

  12. Effect of caffeine on gamma-ray induced G2 arrest in well-synchronized Chinese hamster ovary cells in vitro

    International Nuclear Information System (INIS)

    Masunaga, Shin-ichiro; Keng, P.C.


    G1-rich cells were separated from exponentially growing asynchronous cultured Chinese hamster ovary (CHO-K1) cells by centrifugal elutriation and a Coulter Counter. The G1-rich cells were incubated in medium that contained hydroxyurea (HU) to kill S phase cells and obtain the purest G1/S boundary cells possible. The HU-treated cells were washed, and were again incubated, in medium without HU, to allow these well-synchronized G1/S boundary cells to progress to S and G2/M phases. At various times after release from G1/S boundary, 4 Gy of gamma-ray and/or caffeine was administered to the cells. Eight hours after the removal of HU, cell-cycle analysis was performed with a flow cytometer. G2 arrest induced by gamma-rays was clearly shown when radiation was given earlier than 6.5 hours after HU removal. G2 arrest induced by radiation given 0.5-6.5 hours after HU removal was reduced by caffeine treatment given 6.0-6.5 hours after HU removal. Caffeine released radiation-induced G2 arrest when the radiation was given before the cultured cells entered G2/M phase and when caffeine was applied to the irradiated cells at the time when G1/S boundary cells not treated by radiation or with caffeine entered G2/M phase. Our method of centrifugal elutriation combined with incubation with HU was useful for isolating pure G1/S boundary cells from in vitro asynchronous cultures. (author)

  13. Evaluation of the i-STAT Portable Clinical Analyzer for point-of-care blood testing in the intensive care units of a university children's hospital. (United States)

    Papadea, Christine; Foster, Joyce; Grant, Sharon; Ballard, Sandra A; Cate, John C; Southgate, W Michael; Purohit, Dilip M


    We evaluated the analytical performance of the i-STAT Portable Clinical Analyzer (PCA), a point-of-care testing system consisting of a hand-held analyzer and single-use cartridges that measure different panels of electrolytes, metabolites, blood gases, and hematocrit in 65-100 microl of blood. Our objective was to determine whether PCA measurements at the bedside of patients in the neonatal and pediatric intensive care units of the MUSC Children's Hospital would be as reliable as those performed by the clinical laboratory's primary methods (Radiometer ABL 725 blood gas analyzer; Vitros 750 chemistry analyzer; and Coulter STKS hematology analyzer). Four cartridge types: (a) EC8+ (sodium; potassium; chloride; urea; glucose; pH; blood gases [PO2; pCO2]), (b) EC6+ (sodium; potassium; ionized calcium; glucose; hematocrit; pH), (c) G3+ (pH; PO2; pCO2), and (d) creatinine, were assessed for reproducibility, linearity, and method comparisons using aqueous samples, blood samples supplemented with several analytes, and -225 blood samples from patients. Reproducibility (CV) was good ( or < 3 mo) showed that agreement between the PCA and the primary methods was clinically acceptable. After the PCA was implemented for clinical testing, the observation of discrepant results of creatinine concentrations in neonatal blood samples that would have affected clinical management led to a second creatinine comparison study (59 additional samples) and to our eventual discontinuation of the PCA creatinine assay. This problem notwithstanding, the successful implementation of the PCA is attributed to careful analytical evaluations and ongoing communication with the clinical staff.

  14. On Suspended matter grain size in Baltic sea (United States)

    Bubnova, Ekaterina; Sivkov, Vadim; Zubarevich, Victor


    Suspended matter grain size data were gathered during the 25th research vessel "Akademik Mstislav Keldysh" cruise (1991, September-October). Initial quantitative data were obtained with a use of the Coulter counter and subsequently modified into volume concentrations (mm3/l) for size intervals. More than 80 samples from 15 stations were analyzed (depth range 0-355 m). The main goal of research was to illustrate the spatial variability of suspended matter concentration and dispersion in Baltic Sea. The mutual feature of suspended matter grain size distribution is the logical rise of particle number along with descending of particle's size. Vertical variability of grain size distribution was defined by Baltic Sea hydrological structure, including upper mixed layer - from the surface to the thermocline - with 35 m thick, cold intermediate layer - from the thermocline to the halocline- and bottom layer, which lied under the halocline. Upper layer showed a rise in total suspended matter concentration (up to 0.6 mm3/l), while cold intermediate level consisted of far more clear water (up to 0.1 mm3/l). Such a difference is caused by the thermocline boarding role. Meanwhile, deep bottom water experienced surges in suspended matter concentration owing to the nepheloid layer presence and "liquid bottom" effect. Coastal waters appeared to have the highest amount of particles (up to 5.0 mm3/l). Suspended matter grain size distribution in the upper mixed layer revealed a peak of concentration at 7 μ, which can be due to autumn plankton bloom. Another feature in suspended matter grain size distribution appeared at the deep layer below halocline, where both O2 and H2S were observed and red/ox barrier is. The simultaneous presence of Fe and Mn (in solutions below red/ox barrier) and O2 leads to precipitation of oxyhydrates Fe and Mn and grain size distribution graph peaking at 4.5 μ.

  15. Determination of perfluorobutane in rat blood by automatic headspace capillary gas chromatography and selected ion monitoring mass spectrometry. (United States)

    Hvattum, E; Normann, P T; Oulie, I; Uran, S; Ringstad, O; Skotland, T


    A new contrast agent (Sonazoid; NC100100) for ultrasound imaging has been developed. It is an aqueous suspension of lipid stabilised perfluorobutane (PFB) gas microbubbles. An automatic headspace capillary gas-chromatographic mass spectrometric method using electron impact ionisation was developed for analysis of Sonazoid PFB in rat blood. The calibration standards were gaseous PFB dissolved in ethanol in the range of 0.5-5000 ng PFB. Fluorotrichloromethane (CFC 11) was used as an internal standard of the method and the MS detector was set to single ion monitoring of the base fragment ions of PFB (m/z 69 and 119) and CFC 11 (m/z 101). The calibration graph, made by plotting the peak area ratios of PFB (m/z 69) to CFC 11(m/z 101) against the amount of PFB, was fitted to a second-order polynomial equation with weighting 1/y2 and found to be reproducible. The limit of quantification of the method was set to 0.4 ng PFB. The between-day variation of the method was below 9.2% relative standard deviation (RSD) and the within-day variation of the method was below 7.6% RSD. The accuracy of the method, as compared to Coulter counter, was estimated by determination of PFB in samples where Sonazoid was added to saline and found to range from 91.5% to 105.2%. PFB, added as Sonazoid, was found to be stable for at least 7 months in rat blood samples when stored at -20 degrees C.

  16. Prevalence of Beta-Thalassemia in premarital screening in Al-Hassa, Saudi Arabia

    International Nuclear Information System (INIS)

    Al-Suliman, A.


    The Al-Hassa area is one of the regions in Saudi Arabia where hemoglobinopathies are prevalent. The Saudi Ministry of Health designed a protocol for premarital testing after the royal decree in December 2003. The protocol was implemented in a February 2004 order. The aim of this study was to determine the prevalence of beta-thalassemia trait among subjects coming for premarital screening in the Al-Hassa area. From February 2004 to November 2004, healthy subjects coming to six marriages consultation centers in the Al-Hassa area underwent routine mandatory tests. Subjects were considered to have beta-thalassemia trait if they had a mean corpuscular volume (MCV), 80 fL and/or a mean corpuscular hemoglobin (MCH) 3.2%. Venous blood was taken into ETDA tube and the complete blood count and red blood cell indices were measured by a Coulter automated cell counter on the same day of hemoglobin collection. Electrophoresis was done on cellulose acetate. All Saudi participants (n=8918), including 4218 (47.3%) males and 4700 (52.7%) females were screened. The prevalence of beta-thalassemia trait with high hemoglobin A2 and microcytic hypochromic anemia was 3.4% (307/8918). In countries with a high prelevance of hemoglobinopathies, a premarital screening program is helpful for identification and prevention of high-risk marriages. With a 3.4% prevalence of beta-thalassemia trait in premarital couples, future comprehensive programs are needed to know the actual prevalence of beta-thalassemia in Al-Hassa. (author)


    Directory of Open Access Journals (Sweden)

    V. O. Chernyshenko


    Full Text Available The aim of the research was to study a potential antithrombotic sodium salt of calix[4]arene-methylene-bis-phosphonic acid (С-145 — on activation and aggregation of platelets in vivo, as well as on proliferation and apoptosis of endothelial cells in the cell culture. Effects of calix[4]arene С-145 estimated in vitro after addition to the platelet rich plasma, and in vivo after intravenous injection into rabbit bloodstream in equivalent amounts (46 μM. Aggregation of platelets was induced by adenosine diphosphate and detected using aggregometer Solar AP2110. Platelet shape and cytoplasmic granularity were monitored on COULTER EPICS XL Flow Cytometer. The level of tissuetype plasminogen activator — tPA — was estimated using enzyme-linked immunosorbent assay ELISA. Effects of calix[4]arene C-145 on culture of endotelial cells cells was studied using 3-(4,5-Dimethylthiazol-2-yl-2,5-Diphenyltetrazolium Bromide — MTT-test. The population of proliferative pool of cells (G2/M+S was determined using flow cytometry. Aggregometry and flow cytometry showed that calix[4]arene C-145 did not activate platelets nor affect their aggregation in vitro. However intravenous injection of calix[4]arene C-145 into the bloodstream of healthy rabbits leads to strong inhibition of platelet aggregation and changes of shape and granularity of most of the platelets after 2 hours of administration. Any additional appearance of endothelial cells activation marker tPA in vivo and any inhibition of calix[4]arene C-145 on proliferation of endothelial cells in cell culture did not observe. So calix[4]arene C-145 had strong anti-platelet effect in vivo that was not a result of their direct action on platelets or endothelial cells in vitro. This allowed to assume the possibility of calix[4]arene C-145 use as an effective antithrombotic agent.


    Directory of Open Access Journals (Sweden)

    Ricardo Castillo


    Full Text Available This article is an advance of a wider investigation, in which the objective is to analyze overall maintenance management in public institutions of higher education in the municipality Cabimas, hence in this development describes the elements of the management maintenance present in these institutions. Authors were consulted such as Duffuaa et al. (2010, Clemenza (2010, Zambrano and Leal (2006 , Robbins and Coulter (2005 y Martínez (2007 among others. The research is descriptive, non-experimental   design, transactional  and  field.  We  used  a population   census,  for  a  total  of   twenty-three  (23   research  subjects,  belonging  to  the Universidad   del   Zulia    Núcleo   Costa   Oriental   del   Lago,  Universidad    Nacional Experimental  Rafael  Maria Baralt and  Instituto Universitario Tecnológico  Cabimas. To collect data, a questionnaire was composed of twenty-two (22 items, which was validated by the trial of six (6 experts, with 0.99 reliability by Cronbach alpha coefficient.  We conclude that the elements planning, organizing, scheduling, execution and control, have a high presence in maintenance management, not the human resource and security elements.

  19. Etiology of anemia of blood donor candidates deferred by hematologic screening

    Directory of Open Access Journals (Sweden)

    Michel Alves da Silva


    Full Text Available OBJECTIVE: Iron deficiency is the most common cause of anemia and one of the main factors in the clinical deferral of blood donors. This fact prompted the current study that aimed to determine the prevalence and etiology of anemia in blood donor candidates and to evaluate the hematological screening technique used for the exclusion of these donors. METHODS: This was a prospective study that compared two groups (Anemic and Non-anemic. Initially screening for anemia was performed by manually measuring hemoglobin (Bioclin® Kit; the results were subsequently compared with an automated screening method (Coulter T-890. The etiology was investigated by hemoglobin electrophoresis in alkaline and acid pH, Hb A2 dosage and measurement of the ferritin concentration by immunoagglutination. Differences and associations of interest were analyzed using the Yates and McNemar's Chi-square tests and the Fisher, Mann-Whitney, Wilcoxon and Kruskal-Wallis tests. RESULTS: The deferral rate due to anemia was 4.2%; iron deficiency was identified in 37.5% and beta thalassemia in 9.3% of the excluded candidates. There was a significant discrepancy between the two techniques used to measure hemoglobin with 38.1% of initially deferred donors presenting normal hemoglobin levels by the automated method. CONCLUSION: The results show a high rate of blood donors being deferred for anemia and confirm that iron deficiency is the most prevalent cause. The discrepancies found by comparing screening methods suggest that hemoglobin and hematocrit levels should be confirmed before deferring a donor due to anemia; this may increase supplies in blood banks.

  20. Radioprotection conferred by dextran sulfate given before irradiation in mice

    International Nuclear Information System (INIS)

    Ross, W.M.; Peeke, J.


    Dextran sulfate (DS) has been observed to cause mobilization (fivefold) of hemopoietic stem cells (HSC) and leukocytes, primarily lymphocytes, into the peripheral blood of mice within 2-3 h after intraperitoneal (i.p.) injection. This effect was dose dependent and was prolonged for several hours when the high-molecular-weight version DS500 (500,000 daltons) was used. When DS500 was given 1-3 days before irradiation, hemopoietic recovery was markedly enhanced. Postirradiation injection was ineffective. By ten days after irradiation (7.0 Gy), the number of endogenous spleen colonies (CFUs) and the splenic mass were much larger if DS pretreatment had been given. This effect was dependent on the dose of DS500 and on the time administered, 60 mg/kg producing a maximal effect when given three days before irradiation. DS500 caused a transient anaphylactoid shock, however, in most mice--mild at low doses but potentially lethal at doses above 40 mg/kg (10% mortality within 1-3 days after 60 mg/kg). The following results were obtained with 50 mg/kg, a compromise dose causing minimal mortality (3%) given three days before irradiation. Reticulocyte reappearance was earlier in irradiated mice given DS500, indicating earlier erythropoietic recovery. Some of these reticulocytes were resistant to lysing agents, so their appearance could be detected using the Coulter electronic cell counter, as well as in stained blood smears. The 30-day mortality due to bone marrow failure after irradiation was significantly decreased in DS-treated mice below 9.5 Gy, and the LD50/30 was increased by 0.5 Gy. This study shows that dextran sulfate exerts a radioprotective influence on the hemopoietic system and hence survival when administered prophylactically

  1. Quantificação de células CD 34+ em sangue periférico, produto de aférese e cordão umbilical: estudo comparativo de três diferentes metodologias

    Directory of Open Access Journals (Sweden)

    Bacal Nydia S.


    Full Text Available A quantificação das células CD34+ em sangue periférico é utilizada para determinar o melhor momento de iniciar a aférese, enquanto que na leucoaférese e no sangue de cordão umbilical determinam a quantidade de células CD34+ para o transplante de células progenitoras. O objetivo deste trabalho foi comparar três diferentes metodologias de quantificação de células CD34+. Foram utilizados três diferentes tipos de amostras: a 32 amostras de sangue periférico, coletadas de pacientes estimulados com G-CSF 50 mg/Kg/dose total, sem quimioterapia na mobilização. b 31 amostras de produto de aférese de pacientes estimulados com o mesmo protocolo de G-CSF. c 20 amostras de sangue de cordão coletadas em CPDA-1, por punção de veia umbilical. As amostras permaneceram à temperatura ambiente no máximo até 24h da análise. O citômetro de fluxo utilizado foi o Epics XL-MCL (Coulter com os protocolos de dupla plataforma: ISHAGE e Mulhouse modificado para análise de maior numero de eventos, e o citômetro Imagn 2000 (Biometric Imaging de plataforma única, conforme técnicas recomendadas. Os anticorpos monoclonais utilizados foram: CD45-FITC, CD34-PE, e isotipo IgG1-PE da Immunotech. As análises estatísticas foram: ANOVA e correlação de teste t de Student. Os resultados não apresentaram diferenças estatisticamente significativas nos três métodos.

  2. Spatial variation in the community of insects associated with the flowers of Pachycereus weberi (Caryophyllales: Cactaceae). (United States)

    Figueroa-Castro, Dulce María; Valverde, Pedro Luis; Vite, Fernando; Carrillo-Ruiz, Hortensia


    The positive relationship between productivity and species diversity is well-known. Insect communities associated with the flowers of Cactaceae species represent an interesting system to explore the productivity-diversity relationship because branches facing the equator receive more photosynthetically active radiation and have higher productivity. Thus, flowers with contrasting orientations within an individual, and even within a single branch, might differ in productivity. Therefore, higher abundance, species richness, and diversity are expected for the insect communities associated with south-facing flowers. This hypothesis was tested in Pachycereus weberi (J.M. Coulter) Backeberg (Cactaceae). Insects within flowers with contrasting orientations were collected and its abundance, richness, and diversity were estimated. We also asked if insects prefer big flowers. Thus, flower volume was estimated and regression analyses were conducted to test if there is a positive relationship between flower size and insect abundance. Flower orientation did not affect species richness. However, species abundance and diversity were different in flowers with contrasting orientations. In general, species abundance was higher in flowers facing southwards than in north-facing flowers. On the contrary, species diversity was higher in north-facing flowers. Abundance of Coleoptera was explained by flower volume in south-facing flowers. Contrary to our hypothesis, total diversity was greater in the less productive oriented flowers. Three possible explanations are discussed to explain the low diversity found in the highly productive, south-facing flowers. Our study provides evidence for the effects of productivity on the structure of insect communities at a very small-scale.

  3. Increase of rotation angle of soil layers during plow operation (United States)

    Vasilenko, VV; Afonichev, D. N.; Vasilenko, S. V.; Khakhulin, A. N.


    One of the advantages of plowing is the ability of the plow to hide the weed seeds deep into the soil. The depth of the embankment exceeds 10-12 cm, from there the weeds can not rise to the surface of the soil. They perish halfway. But for this, it is necessary to wrap the soil layers at an angle close to 180 °. Modern ploughs can not turn the layers of soil at an angle of more than 135 °, therefore the plow is required to be equipped with additional working elements. The aim of the study is to create an adaptation to the plow to expand the furrow before laying the next soil layer. In a wide furrow, the formation will completely tip, the previous layer will not interfere with it. The device is a set of vertical shields. Each shield is fixed behind the working body of the plow. It is installed with an angle of attack of 20-25 ° to move the previous layer to expand the furrow by 10-12 cm. The model and industrial samples of the plow have shown improved agrotechnical indicators. The average angle of the formation rotation was 177 °, the burial of plant residues in the soil increased from 61 to 99%. The field surface with blocks more than 5 cm decreased from 36.3 to 13.4%, the height of the ridges decreased from 7 to 4 cm. The force of soil pressure on the shield was measured by a strain gage. It is 130-330 N depending on the depth of processing and the speed of movement. The increase in power costs for the four-hull plow was 190-750 W. The coulters on the plow are unnecessary, and this saves energy more than its increase for shields.

  4. Membrane permeability of the human granulocyte to water, dimethyl sulfoxide, glycerol, propylene glycol and ethylene glycol. (United States)

    Vian, Alex M; Higgins, Adam Z


    Granulocytes are currently transfused as soon as possible after collection because they rapidly deteriorate after being removed from the body. This short shelf life complicates the logistics of granulocyte collection, banking, and safety testing. Cryopreservation has the potential to significantly increase shelf life; however, cryopreservation of granulocytes has proven to be difficult. In this study, we investigate the membrane permeability properties of human granulocytes, with the ultimate goal of using membrane transport modeling to facilitate development of improved cryopreservation methods. We first measured the equilibrium volume of human granulocytes in a range of hypo- and hypertonic solutions and fit the resulting data using a Boyle-van't Hoff model. This yielded an isotonic cell volume of 378 μm(3) and an osmotically inactive volume of 165 μm(3). To determine the permeability of the granulocyte membrane to water and cryoprotectant (CPA), cells were injected into well-mixed CPA solution while collecting volume measurements using a Coulter Counter. These experiments were performed at temperatures ranging from 4 to 37°C for exposure to dimethyl sulfoxide, glycerol, ethylene glycol, and propylene glycol. The best-fit water permeability was similar in the presence of all of the CPAs, with an average value at 21°C of 0.18 μmatm(-1)min(-1). The activation energy for water transport ranged from 41 to 61 kJ/mol. The CPA permeability at 21°C was 6.4, 1.0, 8.4, and 4.0 μm/min for dimethyl sulfoxide, glycerol, ethylene glycol, and propylene glycol, respectively, and the activation energy for CPA transport ranged between 59 and 68 kJ/mol. Copyright © 2013 Elsevier Inc. All rights reserved.

  5. The Oxygen Paradox, the French Paradox, and age-related diseases. (United States)

    Davies, Joanna M S; Cillard, Josiane; Friguet, Bertrand; Cadenas, Enrique; Cadet, Jean; Cayce, Rachael; Fishmann, Andrew; Liao, David; Bulteau, Anne-Laure; Derbré, Frédéric; Rébillard, Amélie; Burstein, Steven; Hirsch, Etienne; Kloner, Robert A; Jakowec, Michael; Petzinger, Giselle; Sauce, Delphine; Sennlaub, Florian; Limon, Isabelle; Ursini, Fulvio; Maiorino, Matilde; Economides, Christina; Pike, Christian J; Cohen, Pinchas; Salvayre, Anne Negre; Halliday, Matthew R; Lundquist, Adam J; Jakowec, Nicolaus A; Mechta-Grigoriou, Fatima; Mericskay, Mathias; Mariani, Jean; Li, Zhenlin; Huang, David; Grant, Ellsworth; Forman, Henry J; Finch, Caleb E; Sun, Patrick Y; Pomatto, Laura C D; Agbulut, Onnik; Warburton, David; Neri, Christian; Rouis, Mustapha; Cillard, Pierre; Capeau, Jacqueline; Rosenbaum, Jean; Davies, Kelvin J A


    A paradox is a seemingly absurd or impossible concept, proposition, or theory that is often difficult to understand or explain, sometimes apparently self-contradictory, and yet ultimately correct or true. How is it possible, for example, that oxygen "a toxic environmental poison" could be also indispensable for life (Beckman and Ames Physiol Rev 78(2):547-81, 1998; Stadtman and Berlett Chem Res Toxicol 10(5):485-94, 1997)?: the so-called Oxygen Paradox (Davies and Ursini 1995; Davies Biochem Soc Symp 61:1-31, 1995). How can French people apparently disregard the rule that high dietary intakes of cholesterol and saturated fats (e.g., cheese and paté) will result in an early death from cardiovascular diseases (Renaud and de Lorgeril Lancet 339(8808):1523-6, 1992; Catalgol et al. Front Pharmacol 3:141, 2012; Eisenberg et al. Nat Med 22(12):1428-1438, 2016)?: the so-called, French Paradox. Doubtless, the truth is not a duality and epistemological bias probably generates apparently self-contradictory conclusions. Perhaps nowhere in biology are there so many apparently contradictory views, and even experimental results, affecting human physiology and pathology as in the fields of free radicals and oxidative stress, antioxidants, foods and drinks, and dietary recommendations; this is particularly true when issues such as disease-susceptibility or avoidance, "healthspan," "lifespan," and ageing are involved. Consider, for example, the apparently paradoxical observation that treatment with low doses of a substance that is toxic at high concentrations may actually induce transient adaptations that protect against a subsequent exposure to the same (or similar) toxin. This particular paradox is now mechanistically explained as "Adaptive Homeostasis" (Davies Mol Asp Med 49:1-7, 2016; Pomatto et al. 2017a; Lomeli et al. Clin Sci (Lond) 131(21):2573-2599, 2017; Pomatto and Davies 2017); the non-damaging process by which an apparent toxicant can activate biological signal

  6. Application of High-Throughput Next-Generation Sequencing for HLA Typing on Buccal Extracted DNA: Results from over 10,000 Donor Recruitment Samples.

    Directory of Open Access Journals (Sweden)

    Yuxin Yin

    Full Text Available Unambiguous HLA typing is important in hematopoietic stem cell transplantation (HSCT, HLA disease association studies, and solid organ transplantation. However, current molecular typing methods only interrogate the antigen recognition site (ARS of HLA genes, resulting in many cis-trans ambiguities that require additional typing methods to resolve. Here we report high-resolution HLA typing of 10,063 National Marrow Donor Program (NMDP registry donors using long-range PCR by next generation sequencing (NGS approach on buccal swab DNA.Multiplex long-range PCR primers amplified the full-length of HLA class I genes (A, B, C from promotor to 3' UTR. Class II genes (DRB1, DQB1 were amplified from exon 2 through part of exon 4. PCR amplicons were pooled and sheared using Covaris fragmentation. Library preparation was performed using the Illumina TruSeq Nano kit on the Beckman FX automated platform. Each sample was tagged with a unique barcode, followed by 2×250 bp paired-end sequencing on the Illumina MiSeq. HLA typing was assigned using Omixon Twin software that combines two independent computational algorithms to ensure high confidence in allele calling. Consensus sequence and typing results were reported in Histoimmunogenetics Markup Language (HML format. All homozygous alleles were confirmed by Luminex SSO typing and exon novelties were confirmed by Sanger sequencing.Using this automated workflow, over 10,063 NMDP registry donors were successfully typed under high-resolution by NGS. Despite known challenges of nucleic acid degradation and low DNA concentration commonly associated with buccal-based specimens, 97.8% of samples were successfully amplified using long-range PCR. Among these, 98.2% were successfully reported by NGS, with an accuracy rate of 99.84% in an independent blind Quality Control audit performed by the NDMP. In this study, NGS-HLA typing identified 23 null alleles (0.023%, 92 rare alleles (0.091% and 42 exon novelties (0.042%.Long

  7. First X-Ray absorption spectroscopy results on Aeolian dust archived in Antarctica and Alpine firn cores (United States)

    Marcelli, A.; Maggi, V.; Cibin, G.; Sala, M.; Marino, F.; Delmonte, B.


    We present the first x-ray absorption spectroscopy (XAS) data at the Fe K-edge collected on insoluble mineral dust from Talos Dome firn core (TDC, 159°04'E, 72°46'S, 2316 m a.s.l., mean accumulation rate 8 g cm-2 yr- 1), drilled in the framework of the International Trans Antarctic Scientific Expedition (ITASE), and from a Colle del Lys 2003 firn core (CDL03, 45°92'N, 7°86'E, 4248m a.s.l., mean accumulation rate 134 g cm-2 yr-1, Lys Glacier, Mt. Rosa, Italy). The low concentration of mineral particles, obtained by filtering each firn core melted samples on Nuclepore membranes in a 1000 class clean room, required a specific procedure to prepare the samples necessary to the successful collection of the XAS data. The firn samples were decontaminated in clean room under laminar flow bench by means of a ceramic knife and discarding the external part of the cores. Analyses of the insoluble particle content were performed by particle counter Beckman CounterãMultisizer III in order to defined concentration and size distribution of particles in each samples. A dedicated HV experimental chamber, devoted to the realization of XAS experiments on very low absorber concentration samples, was developed and realized in the framework of the CryoAlp collaboration at IMONT, the Italian National Institute for Mountains. The original experimental setup, thanks to the presence of an in-vacuum sample micromanipulator and special sample alignment and docking system installed for these experiments at the Stanford Synchrotron Radiation Laboratory at the beamline 6-2, allows both normal-incidence X-ray Fluorescence detection using a Ketek SDD detector having an energy resolution of about 150 eV and extremely low energy detection limit, and Total X-ray Reflection Fluorescence and Absorption Spectroscopy measurements. The high quality of the XANES experiments performed, using both normal incidence and Total Reflection XAS measurements, allowed recognizing iron-inclusion mineral fractions

  8. Application of High-Throughput Next-Generation Sequencing for HLA Typing on Buccal Extracted DNA: Results from over 10,000 Donor Recruitment Samples. (United States)

    Yin, Yuxin; Lan, James H; Nguyen, David; Valenzuela, Nicole; Takemura, Ping; Bolon, Yung-Tsi; Springer, Brianna; Saito, Katsuyuki; Zheng, Ying; Hague, Tim; Pasztor, Agnes; Horvath, Gyorgy; Rigo, Krisztina; Reed, Elaine F; Zhang, Qiuheng


    Unambiguous HLA typing is important in hematopoietic stem cell transplantation (HSCT), HLA disease association studies, and solid organ transplantation. However, current molecular typing methods only interrogate the antigen recognition site (ARS) of HLA genes, resulting in many cis-trans ambiguities that require additional typing methods to resolve. Here we report high-resolution HLA typing of 10,063 National Marrow Donor Program (NMDP) registry donors using long-range PCR by next generation sequencing (NGS) approach on buccal swab DNA. Multiplex long-range PCR primers amplified the full-length of HLA class I genes (A, B, C) from promotor to 3' UTR. Class II genes (DRB1, DQB1) were amplified from exon 2 through part of exon 4. PCR amplicons were pooled and sheared using Covaris fragmentation. Library preparation was performed using the Illumina TruSeq Nano kit on the Beckman FX automated platform. Each sample was tagged with a unique barcode, followed by 2×250 bp paired-end sequencing on the Illumina MiSeq. HLA typing was assigned using Omixon Twin software that combines two independent computational algorithms to ensure high confidence in allele calling. Consensus sequence and typing results were reported in Histoimmunogenetics Markup Language (HML) format. All homozygous alleles were confirmed by Luminex SSO typing and exon novelties were confirmed by Sanger sequencing. Using this automated workflow, over 10,063 NMDP registry donors were successfully typed under high-resolution by NGS. Despite known challenges of nucleic acid degradation and low DNA concentration commonly associated with buccal-based specimens, 97.8% of samples were successfully amplified using long-range PCR. Among these, 98.2% were successfully reported by NGS, with an accuracy rate of 99.84% in an independent blind Quality Control audit performed by the NDMP. In this study, NGS-HLA typing identified 23 null alleles (0.023%), 92 rare alleles (0.091%) and 42 exon novelties (0.042%). Long

  9. Generation of Microcellular Biodegradable Polycaprolactone Foams in Supercritical Carbon Dioxide

    Institute of Scientific and Technical Information of China (English)

    Xu Qun; Ren Xian-wen; Chang Yu-ning; Yu Long; Wang Jing-wu


    Present now the application of microcellular polymeric materials in biomedical field is growing rapidly, as that of guided tissue regeneration and cell transplantation. As far as guided tissue regeneration is concerned, porous implants are used as size selective membrane to promote the growth of a special tissue in a healing site. Ideally, the implant should be inherently biocompatible,have well-defined cell size and be resorbable with appropriate biodegradation rates.Poly(a-caprolactone) (PCL) is a kind of materials suit for the demands above. PCL is biocompatible and biodegradable aliphatic polyester which is nontoxic for living organisms and bioresorbable after a period of implantation. Because of its unique combination of biocompatibility, permeability and biodegradability, PCL and some of its copolymer with lactides and glycolide have been widely applied in medicine as artificial skin, artificial bone and containers for sustained drug release.Goel and Beckman have reported a new method to generate microcellular poly(methy l methacrylate) foams in which the samples are saturated with CO2 under a series of supercritical (SC)conditions, and then the system is rapidly depressurized to atmospheric pressure at constant temperature. Unlike traditional methods, it reduces glass-transition temperature (Tg) of the mixture to below the experimental temperature rather than directly heat the system above Tg. In this process of nucleation, no phase separation occurs as well as no phase boundary meets, so the cellular structure of the foam can be retained better.In this work, we have generated PCL foams by using supercritical CO2. Because of the low glass transition temperature (Tg = -60 ℃) of PCL far below the ice point, the experimental temperature in our study is much higher than Tg, which is different from the studies by others before. A series of variable factors on the foam structure as saturation temperature, saturation pressure, saturation time and depressurization

  10. Oxidant-Antioxidant Balance in Severe Brain Injury

    Directory of Open Access Journals (Sweden)

    N. N. Yepifantseva


    Full Text Available Objective: to study the time course of changes in oxidative status parameters and their relationship with inflammation mediators in the acute period of severe brain injury (SBI. Subjects and methods. One hundred and thirteen patients aged 17—67 years were examined. The injury was closed and open in 54 (47.8% and 59 (52.2% patients, respectively. Severe brain contusions were observed in 47 patients, diffuse axonal lesions were seen in 2, and intracranial hematomas were present in 64 patients. The Glasgow coma scores for admission consciousness loss were 6.8±0.25. A control group comprised 23 healthy individuals. The significance of differences was estimated by Student’s test, Wilcoxon-Mann-Whitney, test, Spearman’s correlation test. Venous blood samples were used to study total oxidative activity (TOA and total antioxidative activity (TAA, diene conjugates, lactic acid, albumin, transferrin (TF, ceruloplasmin, C-reactive protein, and lactoferrin (LF were measured in venous blood on disease days 1, 4, 7, 10, 14, and 21. The profile of plasma cytokines (IL-1j8, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNF-а, and IFN-y was studied by flow fluorometry on a Cytomics FC 500 cytofluorometer (Beckman Counlter, USA (reagents were from Bender Medsystems, Austria. Results. In SBI, there was an increase in oxidants, a reduction in antioxidant activity, and lipid peroxidation activation, which were closely related. The oxidation coefficient (TOA/TAA was 40 times greater than the normal values on days 7 to 10. The oxidation parameters were found to be associated with inflammation and cytokine-mediated immunological reactions. The time course of changes in the study proteins was characteristic for systemic inflammation and there was an association with oxidative processes only for ceruloplasm. TF was found to have an association with IL-5 and IL-10, which reflects its involvement in immunological reactions. The association with hypoxia was

  11. Evaluating the clinical accuracy of two continuous glucose sensors using continuous glucose-error grid analysis. (United States)

    Clarke, William L; Anderson, Stacey; Farhy, Leon; Breton, Marc; Gonder-Frederick, Linda; Cox, Daniel; Kovatchev, Boris


    To compare the clinical accuracy of two different continuous glucose sensors (CGS) during euglycemia and hypoglycemia using continuous glucose-error grid analysis (CG-EGA). FreeStyle Navigator (Abbott Laboratories, Alameda, CA) and MiniMed CGMS (Medtronic, Northridge, CA) CGSs were applied to the abdomens of 16 type 1 diabetic subjects (age 42 +/- 3 years) 12 h before the initiation of the study. Each system was calibrated according to the manufacturer's recommendations. Each subject underwent a hyperinsulinemic-euglycemic clamp (blood glucose goal 110 mg/dl) for 70-210 min followed by a 1-mg.dl(-1).min(-1) controlled reduction in blood glucose toward a nadir of 40 mg/dl. Arterialized blood glucose was determined every 5 min using a Beckman Glucose Analyzer (Fullerton, CA). CGS glucose recordings were matched to the reference blood glucose with 30-s precision, and rates of glucose change were calculated for 5-min intervals. CG-EGA was used to quantify the clinical accuracy of both systems by estimating combined point and rate accuracy of each system in the euglycemic (70-180 mg/dl) and hypoglycemic (<70 mg/dl) ranges. A total of 1,104 data pairs were recorded in the euglycemic range and 250 data pairs in the hypoglycemic range. Overall correlation between CGS and reference glucose was similar for both systems (Navigator, r = 0.84; CGMS, r = 0.79, NS). During euglycemia, both CGS systems had similar clinical accuracy (Navigator zones A + B, 88.8%; CGMS zones A + B, 89.3%, NS). However, during hypoglycemia, the Navigator was significantly more clinically accurate than the CGMS (zones A + B = 82.4 vs. 61.6%, Navigator and CGMS, respectively, P < 0.0005). CG-EGA is a helpful tool for evaluating and comparing the clinical accuracy of CGS systems in different blood glucose ranges. CG-EGA provides accuracy details beyond other methods of evaluation, including correlational analysis and the original EGA.

  12. Content Of 2,4-D-14C Herbicide Residue In Water And Soil Of Irrigated Rice Field System

    International Nuclear Information System (INIS)

    Chairul, Sofnie M.; Djabir, Elida; Magdalena, Nelly


    The investigation of 2,4-D exp.-14C herbicide residue in water and soil of irrigated rice field system was carried out. Rice plant and weeds (Monochoria vaginalis Burn. F. Presl) were planted in 101 buckets using two kinds of soil condition, I.e. normal soil and 30 % above normal compact soil. After one week planting, the plants were sprayed with 1 u Ci of 2,4-D exp.-14C and 0,4 mg non labeled 2,4-D. The herbicide residue content was determined 0, 2, 4, 8 and 10 weeks after spraying with 2,4-D herbicide. The analysis was done using Combustion Biological Oxidizer merk Harvey ox-400, and counted with Liquid Scintillation Counter merk Beckman model LS-1801. The results indicates that the herbicide contents in water and soil decrease from the first spraying with herbicide until harvest herbicide Residue content in water after harvest was 0.87 x 10 exp.-6 ppm for soil normal condition, and 0.59 x 10 exp.-6 pm for the soil 30 % up normal condition, while herbicide content in soil was 1.54 x 10 exp.-6 ppm for soil normal condition and 1.48 x 10 exp.-6 ppm for soil 30 % up normal. 2,4-D herbicide residue content in rice after harvest was 0.27 x 10 exp.-6 ppm for normal soil condition and 0.25 x 10 exp.-6 ppm for the soil 30 % up normal. 2,4-D herbicide residue content in roots and leaves of weeds after harvest were respectively 0.29 x 10 exp.-6 ppm and 0.18 x 10 exp.-6 for normal soil condition, while for 30 % up normal soil were 0.25 x 10 exp.-5 ppm and 0.63 x 10 exp.-7 ppm. This result indicates that there is no effect pollution to surrounding area, because the herbicide content is still bellow the allowed detection limit, 0.05 ppm

  13. Antigen Expression on Blast Cells and Hematological Parameters at Presentation in Acute Lymphoblastic Leukemia Patients

    International Nuclear Information System (INIS)

    Naeem, S.; Bukhari, M. H.


    Objective: To analyze the expression of various antigens on the leukemic blasts and to determine the hematological parameters, in Acute Lymphoblastic Leukemia (ALL) patients at presentation. Study Design: Observational study. Place and Duration of Study: King Edward Medical University, Lahore and Hameed Latif Hospital, Lahore, from February 2013 to March 2014. Methodology: A total of 50 newly diagnosed and untreated patients of ALL were selected from Mayo Hospital and Hameed Latif Hospital. These patients included both genders and all age groups. Hemoglobin, total leukocyte count and platelet count were determined on hematology analyser-Sysmex-Kx-2I. Blast cell percentage was estimated on Giemsa stained blood smears. Immuno phenotyping was done on bone marrow samples by 5 colour flow cytometery on Beckman Counter Navious Flow cytometer. An acute leukemia panel of 23 antibodies was used. The data was entered and analyzed in SPSS version 22. Results: Of the 50 ALL patients, 36 (72 percentage) were B-ALL and 14 (28 percentage) T-ALL. There were 18 (36 percentage) children and 32 (64 percentage) adults. T-ALL included 22 percentage of the childhood and 31 percentage of the adult cases. Immuno phenotypic analysis showed that CD19, CD79a and CD20 were B-lineage specific markers whereas cCD3, CD3 and CD5 were T-lineage specific. CD10 was the most sensitive marker for B-ALL and CD7 was the most sensitive marker of T-ALL. TdT was expressed in 92 percentage B-ALL and 71 percentage T-ALL cases, CD34 in 58 percentage and 43 percentage cases and CD45 in 83 percentage and 100 percentage respectively. High leukocyte count (> 50 x 109/L) was present in 58 percentage cases. Hemoglobin was < 10 g/dl in 74 percentage patients and platelet count was below 20 x 109/Lin 12 percentage patients. Leukocyte count, hemoglobin, platelet count and blast cell percentage did not show a significant difference in the two ALL immuno types. Conclusion: The frequency of T-ALL is higher in childhood

  14. Comparison of manual and automated cell counts in EDTA preserved synovial fluids. Storage has little influence on the results. (United States)

    Salinas, M; Rosas, J; Iborra, J; Manero, H; Pascual, E


    To determine the precision and agreement of synovial fluid (SF) cell counts done manually and with automated counters, and to determine the degree of variability of the counts in SF samples, kept in the tubes used for routine white blood cell (WBC) counts--which use liquid EDTA as anticoagulant--at 24 and 48 hours at 4 degrees C, and at room temperature. To determine precision, cell counts were repeated 10 times--both manually and by an automated counter--in a SF sample of low, medium, and high cellularity. The variances were calculated to determine the interobserver variation in two manual (M1,M2) and two automated cell counts (C1,C2). The agreement between a manual (M1) and automated counter (C1) results, was analysed by the Bland and Altman method and the difference against the mean of the two methods was plotted. Then, the mean difference between the two methods was estimated and the standard deviation of the difference. To determine the effects of storage, SF samples were kept in a refrigerator at 4 degrees C, and at room temperature; cell counts were done manually (M1) and automatically (C1) at 24 and 48 hours and the changes analysed by the Bland and Altman method. The variances were compared using an F test. (1) Precision. With the manual technique, the coefficients of variation were 27.9%, 14%, and 10.7% when used for counting the SF with low (270), medium (6200), and high cellularities (25,000). With the automated technique the coefficients of variation were 20%, 3.4%, and 2.9% in the same SF samples. In the fluids of medium and high cellularity, the variances of the automated cell counts were significatively lower (F test, p automated counter. (4) Influence of storage. The coulter counts of SF samples preserved at 4 degrees C showed less variance (F test, p Automated cell count of the SF offers advantages: it gives higher precision and consumes less time. The stability of the samples preserved in the EDTA tubes used for routine WBC counts is of

  15. Science education through open and distance learning at Higher Education level

    Directory of Open Access Journals (Sweden)

    Amrita NIGAM


    Full Text Available Abstract The changes faced by the society in the past few decades brought revolution in all areas. The job requirements have undergone change tremendously. The emergence of e-culture, e-education, e-governance, e-training, e-work sites and so on questioned the capacity of conventional face to face education in catering to all and relevance of existing job related skills to a great extent in the emerging global society. Today, every one has to update his/her educational and/or professional skills and competencies to cope up with the emerging work challenges. This is more so in the field of science and technology. At the same time, it is impossible to cater to educational and training opportunities to one and all those who aspire for it through the conventional set up. The distance and open learning (ODL seems to be one of the viable alternatives. Today, the success and viability of ODL is accepted globally. Coulter (1989, through a study demonstrated that ODL is a cost-effective medium in providing educational opportunities. Similarly Holmberg (1981 also mentioned ODL as a systematic teaching-learning medium by using variety of medium for imparting learning. The present study is an attempt to study the experiences of the open science learners of IGNOU on different aspect of the science higher education. Here a questionnaire was used to collect the data and responses from 81 students enrolled for B. Sc. from IGNOU were collected. The findings of the study reported that society has undergone drastic changes in the last few decades. The revolution led due to Information and Communication Technologies (ICTs have widely affected all aspects of society. The emerging jobs require entirely new skills and competencies i.e., employment in BPOs or switching over to e-governance, e-Banking and e- based sectors. Even e-learning has made numerous expectations from teachers and other personnel. The use of ICTs in almost every field needs adequately trained

  16. WE-DE-201-07: Measurement of Real-Time Dose for Tandem and Ovoid Brachytherapy Procedures Using a High Precision Optical Fiber Radiation Detector

    Energy Technology Data Exchange (ETDEWEB)

    Belley, MD [Duke University, Durham, NC (United States); Current Address Rhode Island Hospital, Providence, RI (United States); Faught, A; Subashi, E; Chino, JP; Craciunescu, O [Duke University Medical Center, Durham, NC (United States); Moore, B; Langloss, B; Therien, MJ [Duke University, Durham, NC (United States); Yoshizumi, TT [Duke University, Durham, NC (United States); Duke University Medical Center, Durham, NC (United States)


    for performing patient-specific quality assurance for Ir-192 HDR GYN procedures. Funding from Coulter Foundation, Duke Bio-medical Engineering. Company is being created around the detector technology. Duke holds patents on the technology.

  17. The WAIS Melt Monitor: An automated ice core melting system for meltwater sample handling and the collection of high resolution microparticle size distribution data (United States)

    Breton, D. J.; Koffman, B. G.; Kreutz, K. J.; Hamilton, G. S.


    profiles to guide the melting process. The combination of these data allow us to analyze melt head performance, minimize outer-to-inner fraction contamination and avoid melt head flooding. The WAIS Melt Monitor system allows the collection of real-time, sub-annual microparticle and electrical conductivity data while producing and storing enough sample for traditional Coulter-Counter particle measurements as well long term acid leaching of bioactive metals (e.g., Fe, Co, Cd, Cu, Zn) prior to chemical analysis.

  18. Cholesterol and phytosterols differentially regulate the expression of caveolin 1 and a downstream prostate cell growth-suppressor gene (United States)

    Ifere, Godwin O.; Equan, Anita; Gordon, Kereen; Nagappan, Peri; Igietseme, Joseph U.; Ananaba, Godwin A.


    Background The purpose of our study was to show the distinction between the apoptotic and anti-proliferative signaling of phytosterols and cholesterol enrichment in prostate cancer cell lines, mediated by the differential transcription of caveolin-1, and N-myc downstream regulated gene1 (NDRG1), a pro-apoptotic androgen-regulated tumor suppressor. Methods PC-3 and DU145 cells were treated with sterols (cholesterol and phytosterols) for 72 h, followed by trypan blue dye exclusion measurement of necrosis and cell growth measured with a Coulter counter. Sterol induction of cell growth-suppressor gene expression was evaluated by mRNA transcription using RT-PCR, while cell cycle analysis was performed by FACS analysis. Altered expression of Ndrg1 protein was confirmed by Western blot analysis. Apoptosis was evaluated by real time RT-PCR amplification of P53, Bcl-2 gene and its related pro- and anti-apoptotic family members. Results Physiological doses (16 µM) of cholesterol and phytosterols were not cytotoxic in these cells. Cholesterol enrichment promoted cell growth (Pphytosterols significantly induced growth-suppression (Pphytosterols decreased mitotic subpopulations. We demonstrated for the first time that cholesterols concertedly attenuated the expression of caveolin-1(cav-1) and NDRG1 genes in both prostate cancer cell lines. Phytosterols had the opposite effect by inducing overexpression of cav-1, a known mediator of androgen-dependent signals that presumably control cell growth or apoptosis. Conclusions Cholesterol and phytosterol treatment differentially regulated the growth of prostate cancer cells and the expression of p53 and cav-1, a gene that regulates androgen-regulated signals. These sterols also differentially regulated cell cycle arrest, downstream pro-apoptotic androgen-regulated tumor-suppressor, NDRG1 suggesting that cav-1 may mediate pro-apoptotic NDRG1 signals. Elucidation of the mechanism for sterol modulation of growth and apoptosis signaling

  19. Proliferative vitreo-retinal disorders: experimental models in vivo and in vitro. (United States)

    Martini, B


    The aim of the present thesis was to develop, refine, and assess experimental models for the study of proliferative vitreo-retinal disorders. An intravitreal injection of a colloidal solution of microparticles was used in the primate eye to produce pathologic changes including intraocular cell invasion, cell proliferation, neovascularization, collagen synthesis, and tractional retinal detachment. In a separate primate model for laser-induced subretinal neovascularization, the origin and the occurrence of macrophages was evaluated. Examinations were performed using ophthalmoscopy, slit-lamp microscopy, light microscopy, and transmission electron microscopy. Cell cultures were employed to study the effects of vitreous humor and macrophages on the proliferation of cultured retinal pigment epithelial (RPE) cells and cultured fibroblasts using a Coulter counter. Morphologic changes were documented by phase micrography. A quantitative estimation of the extracellular matrix deposition of fibrous proteins by macrophage-modulated RPE cells as well as by vitreous-modulated RPE cells was done using enzymatic digestion and radioactive labeling techniques. A qualitative analysis of the types of collagen that was deposited in the extracellular matrices by vitreous modulated cultures was also made using indirect immunofluorescence. Using a newly developed RPE cell specific monoclonal antibody, the avidin-biotin-peroxidase labeling technique was finally employed to test the phenotypic epitope expression of macrophage-modulated and non-modulated RPE cells. A new experimental in vivo model for pathologic changes that characterize proliferative vitreo-retinal disorders was developed in the primate eye. In the model for laser-induced subretinal neovascularization, macrophages were shown to be principally recruited from the systemic circulation. Using cell cultures, it was found that both macrophage-conditioned medium and vitreous humor, separately or combined, exert mitogenic effects

  20. Effects of CO2 on particle size distribution and phytoplankton abundance during a mesocosm bloom experiment (PeECE II

    Directory of Open Access Journals (Sweden)

    M. Schartau


    Full Text Available The influence of seawater carbon dioxide (CO2 concentration on the size distribution of suspended particles (2–60 μm and on phytoplankton abundance was investigated during a mesocosm experiment at the large scale facility (LFS in Bergen, Norway, in the frame of the Pelagic Ecosystem CO2 Enrichment study (PeECE II. In nine outdoor enclosures the partial pressure of CO2 in seawater was modified by an aeration system to simulate past (~190 μatm CO2, present (~370 μatm CO2 and future (~700 μatm CO2 CO2 conditions in triplicates. Due to the initial addition of inorganic nutrients, phytoplankton blooms developed in all mesocosms and were monitored over a period of 19 days. Seawater samples were collected daily for analysing the abundance of suspended particles and phytoplankton with the Coulter Counter and with Flow Cytometry, respectively. During the bloom period, the abundance of small particles (2 levels. At that time, a direct relationship between the total-surface-to-total-volume ratio of suspended particles and DIC concentration was determined for all mesocosms. Significant changes with respect to the CO2 treatment were also observed in the phytoplankton community structure. While some populations such as diatoms seemed to be insensitive to the CO2 treatment, others like Micromonas spp. increased with CO2, or showed maximum abundance at present day CO2 (i.e. Emiliania huxleyi. The strongest response to CO2 was observed in the abundance of small autotrophic nano-plankton that strongly increased during the bloom in the past CO2 mesocosms. Together, changes in particle size distribution and phytoplankton community indicate a complex interplay between the ability of the cells to physiologically respond to changes in CO2 and size selection. Size of cells is of general importance for a variety of processes in marine systems such as diffusion-limited uptake of substrates, resource allocation, predator-prey interaction, and gravitational settling

  1. A visual basic program to generate sediment grain-size statistics and to extrapolate particle distributions (United States)

    Poppe, L.J.; Eliason, A.H.; Hastings, M.E.


    Measures that describe and summarize sediment grain-size distributions are important to geologists because of the large amount of information contained in textural data sets. Statistical methods are usually employed to simplify the necessary comparisons among samples and quantify the observed differences. The two statistical methods most commonly used by sedimentologists to describe particle distributions are mathematical moments (Krumbein and Pettijohn, 1938) and inclusive graphics (Folk, 1974). The choice of which of these statistical measures to use is typically governed by the amount of data available (Royse, 1970). If the entire distribution is known, the method of moments may be used; if the next to last accumulated percent is greater than 95, inclusive graphics statistics can be generated. Unfortunately, earlier programs designed to describe sediment grain-size distributions statistically do not run in a Windows environment, do not allow extrapolation of the distribution's tails, or do not generate both moment and graphic statistics (Kane and Hubert, 1963; Collias et al., 1963; Schlee and Webster, 1967; Poppe et al., 2000)1.Owing to analytical limitations, electro-resistance multichannel particle-size analyzers, such as Coulter Counters, commonly truncate the tails of the fine-fraction part of grain-size distributions. These devices do not detect fine clay in the 0.6–0.1 μm range (part of the 11-phi and all of the 12-phi and 13-phi fractions). Although size analyses performed down to 0.6 μm microns are adequate for most freshwater and near shore marine sediments, samples from many deeper water marine environments (e.g. rise and abyssal plain) may contain significant material in the fine clay fraction, and these analyses benefit from extrapolation.The program (GSSTAT) described herein generates statistics to characterize sediment grain-size distributions and can extrapolate the fine-grained end of the particle distribution. It is written in Microsoft

  2. Determination of reference ranges for full blood count parameters in neonatal cord plasma in Hilla, Babil, Iraq

    Directory of Open Access Journals (Sweden)

    Al-Marzoki JM


    Full Text Available Jasim M Al-Marzoki1, Zainab W Al-Maaroof2, Ali H Kadhum31Department of Pediatrics, 2Department of Pathology, Babylon Medical College, 3Babylon Gynecology and Pediatric Teaching Hospital, Hilla, IraqBackground: The health of an individual is known to vary in different countries, in the same country at different times, and in the same individuals at different ages. This means that the condition of individuals must be related to or compared with reference data. Determination of a reference range for the healthy term newborn is clinically important in terms of various complete blood count parameters. The purpose of this study was to establish a local reference range for full blood count parameters in neonatal cord plasma in Hilla, Babil, Iraq.Methods: A total of 220 mothers and their neonates were enrolled in this cross-sectional study from February 2011 to January 2012. Maternal inclusion criteria were age 15–45 years, an uneventful pregnancy, and hemoglobin ≥ 10 g. Neonatal inclusion criteria were full term (37–42 weeks and normal birth weight. The umbilical cord was immediately clamped after delivery of the baby; 3 mL of cord blood was then taken from the umbilical vein and collected in a tube containing ethylenediamine tetra-acetic acid, its plasma was analyzed for full blood count parameters by standard Coulter gram, and the differential leukocyte count was done manually.Results: Mean neonatal hemoglobin was 13.88 ± 1.34 (range 11–17.3 g/dL and mean white cell count was 10.12 ± 2.8 (range 3.1–21.6 × 109/L. Mean platelet count was 267.63 ± 60.62 (range 152–472 × 109/L. No significant differences in red cell, white cell, or platelet counts were found between males and females, except for neutrophil count. The current study shows lower levels of hemoglobin, white cells, and red cells compared with other studies, and there is agreement with some studies and disagreement with others concerning platelet count.Conclusion: Most results

  3. Determination of reference ranges for full blood count parameters in neonatal cord plasma in Hilla, Babil, Iraq (United States)

    Al-Marzoki, Jasim M; Al-Maaroof, Zainab W; Kadhum, Ali H


    Background The health of an individual is known to vary in different countries, in the same country at different times, and in the same individuals at different ages. This means that the condition of individuals must be related to or compared with reference data. Determination of a reference range for the healthy term newborn is clinically important in terms of various complete blood count parameters. The purpose of this study was to establish a local reference range for full blood count parameters in neonatal cord plasma in Hilla, Babil, Iraq. Methods A total of 220 mothers and their neonates were enrolled in this cross-sectional study from February 2011 to January 2012. Maternal inclusion criteria were age 15–45 years, an uneventful pregnancy, and hemoglobin ≥ 10 g. Neonatal inclusion criteria were full term (37–42 weeks) and normal birth weight. The umbilical cord was immediately clamped after delivery of the baby; 3 mL of cord blood was then taken from the umbilical vein and collected in a tube containing ethylenediamine tetra-acetic acid, its plasma was analyzed for full blood count parameters by standard Coulter gram, and the differential leukocyte count was done manually. Results Mean neonatal hemoglobin was 13.88 ± 1.34 (range 11–17.3) g/dL and mean white cell count was 10.12 ± 2.8 (range 3.1–21.6) × 109/L. Mean platelet count was 267.63 ± 60.62 (range 152–472) × 109/L. No significant differences in red cell, white cell, or platelet counts were found between males and females, except for neutrophil count. The current study shows lower levels of hemoglobin, white cells, and red cells compared with other studies, and there is agreement with some studies and disagreement with others concerning platelet count. Conclusion Most results in the current study were within the reference range. The hematological reference values for Iraqi neonatal cord plasma need to be confirmed by larger numbers of blood samples and by collecting samples from

  4. Role of Tamm-Horsfall protein and uromodulin in calcium oxalate crystallization

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    Carvalho M.


    Full Text Available One of the defenses against nephrolithiasis is provided by macromolecules that modulate the nucleation, growth, aggregation and retention of crystals in the kidneys. The aim of the present study was to determine the behavior of two of these proteins, Tamm-Horsfall and uromodulin, in calcium oxalate crystallization in vitro. We studied a group of 10 male stone formers who had formed at least one kidney stone composed of calcium oxalate. They were classified as having idiopathic nephrolithiasis and had no well-known metabolic risk factors involved in kidney stone pathogenesis. Ten normal men were used as controls, as was a group consisting of five normal women and another consisting of five pregnant women. Crystallization was induced by a fixed supersaturation of calcium oxalate and measured with a Coulter Counter. All findings were confirmed by light and scanning electron microscopy. The number of particulate material deposited from patients with Tamm-Horsfall protein was higher than that of the controls (P<0.001. However, Tamm-Horsfall protein decreased the particle diameter of the stone formers when analyzed by the mode of the volume distribution curve (P<0.002 (5.64 ± 0.55 µm compared to 11.41 ± 0.48 µm of uromodulin; 15.94 ± 3.93 µm and 12.45 ± 0.97 µm of normal men Tamm-Horsfall protein and uromodulin, respectively; 8.17 ± 1.57 µm and 9.82 ± 0.95 µm of normal women Tamm-Horsfall protein and uromodulin, respectively; 12.17 ± 1.41 µm and 12.99 ± 0.51 µm of pregnant Tamm-Horsfall protein and uromodulin, respectively. Uromodulin produced fewer particles than Tamm-Horsfall protein in all groups. Nonetheless, the total volume of the crystals produced by uromodulin was higher than that produced by Tamm-Horsfall protein. Our results indicate a different effect of Tamm-Horsfall protein and uromodulin. This dual behavior suggests different functions. Tamm-Horsfall protein may act on nucleation and inhibit crystal aggregation, while

  5. A granulometry and secondary mineral fingerprint of chemical weathering in periglacial landscapes and its application to blockfield origins (United States)

    Goodfellow, Bradley W.


    A review of published literature was undertaken to determine if there was a fingerprint of chemical weathering in regoliths subjected to periglacial conditions during their formation. If present, this fingerprint would be applied to the question of when blockfields in periglacial landscapes were initiated. These blocky diamicts are usually considered to represent remnants of regoliths that were chemically weathered under a warm, Neogene climate and therefore indicate surfaces that have undergone only a few metres to a few 10s of metres of erosion during the Quaternary. Based on a comparison of clay and silt abundances and secondary mineral assemblages from blockfields, other regoliths in periglacial settings, and regoliths from non-periglacial settings, a fingerprint of chemical weathering in periglacial landscapes was identified. A mobile regolith origin under, at least seasonal, periglacial conditions is indicated where clay(%) ≤ 0.5*silt(%) + 8 across a sample batch. This contrasts with a mobile regolith origin under non-periglacial conditions, which is indicated where clay(%) ≥ 0.5*silt(%) - 6 across a sample batch with clay(%) ≥ 0.5*silt(%) + 8 in at least one sample. A range of secondary minerals, which frequently includes interstratified minerals and indicates high local variability in leaching conditions, is also commonly present in regoliths exposed to periglacial conditions during their formation. Clay/silt ratios display a threshold response to temperature, related to the freezing point of water, but there is little response to precipitation or regolith residence time. Lithology controls clay and silt abundances, which increase from felsic, through intermediate, to mafic compositions, but does not control clay/silt ratios. Use of a sedigraph or Coulter Counter to determine regolith granulometry systematically indicates lower clay abundances and intra-site variability than use of a pipette or hydrometer. In contrast to clay/silt ratios, secondary

  6. Cytometric evaluation of intracellular IFN-γ and IL-4 levels in thyroid follicular cells from patients with autoimmune thyroid diseases

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    Bossowski Artur


    Full Text Available Abstract Background In recent few years is underlined that altered balance of pro- and anti-inflammatory cytokines play an important role in the pathogenesis of AITD. The aim of this study was to estimate intracellular INF-γ and IL-4 levels in thyroid-infiltrating lymphocytes and thyrocytes isolated from thyroid tissues in 54 adolescent patients aged 8-21 years, with Graves' disease (GD; n = 18, Hashimoto's thyroiditis (HT; n = 18 and non-toxic multinodular goiter (NTMG; n = 18. Methods Fresh thyroid tissues were taken on culture medium RPMI -1640, it was mechanically prepared. In next step were added cell activators -12- myristate 13- the acetate (PMA and Ionomycin as well as the inhibitor of transportation of proteins - Breferdin A. They were cultured 24 hours in 50 ml flasks at 37°C in a 5-95% CO2-air water-saturated atmosphere. After that, thyrocytes were identified by mouse mAb directed against human TPO epitope 64 conjugated with rabbit anti-mouse antibodies IgG (Fab'2 labeled by FITC. After incubation at room temperature to each of samples added reagent A fixative the cellular membrane. In next step into the cell suspensions were added reagent B to permeabilization of cellular membrane and specific anti-IL-4-PE or anti-IFN-γ-PE mAbs. Identification of intracellular cytokines in T lymphocytes was performed in the same procedure with application of anti-CD4-PerCP and anti-CD8-PerCP mAbs specific for T lymphocytes. The cells were analyzed in a flow cytometry (Coulter EPICS XL. Results In examined group of patients with GD we observed statistically significant higher mean percentage of cells with phenotype CD4+IL-4 (p Conclusions We conclude that human thyrocytes in autoimmune thyroid disorders could be a source of cytokine production and that their activation influences local interaction with T lymphocytes inflowing to the thyroid gland.

  7. Sedimentology, geochemistry and rock magnetic properties of beach sands in Galapagos Islands - implications for nesting marine turtles (United States)

    Perez-Cruz, L.; Urrutia-Fucugauchi, J.; Vazquez-Gutierrez, F.; Carranza-Edwards, A.


    Marine turtles are well known for their navigation ability in the open ocean and fidelity to nesting beaches. Green turtle adult females migrate from foraging areas to island nesting beaches, traveling hundreds or thousands of kilometers each way. The marine turtle breeding in the Galapagos Islands is the Green Sea Turtle (Chelonia mydas agassisi); fairly common throughout the islands but with nesting sites located at Las Bachas (Santa Cruz), Barahona and Quinta Playa (Isabela), Salinas (Baltra), Gardner Bay (Española) and Bartolomé Islet. In order to characterize and to identify the geochemical signature of nesting marine turtle beaches in Galapagos Islands, sedimentological, geochemical and rock magnetic parameters are used. A total of one hundred and twenty sand samples were collected in four beaches to relate compositional characteristics between equivalent areas, these are: Las Bachas, Salinas, Barahona and Quinta Playa. Grain size is evaluated using laser particle analysis (Model Coulter LS 230). Bulk ICP-MS geochemical analysis is performed, following trace elements are analyzed: Al, V, Cr, Co, Ni, Cu, Zn, Cd, Ba, Pb, Fe, Mn, K, Na, Mg, Sr, Ca and Hg; and low-field magnetic susceptibility is measured in all samples at low and high frequencies. Granulometric analysis showed that Barahona and Quinta Playa are characterized for fine grained sands. In contrast, Salinas and Las Bachas exhibit medium to coarse sands. Trace metals concentrations and magnetic susceptibility show different distribution patterns in the beach sands. Calcium is the most abundant element in the samples. In particular, Co, K, and Na show similar concentrations in the four beaches. Las Bachas beach shows highest concentrations of Pb and Hg (maximum values 101.1 and 118.5 mg/kg, respectively), we suggest that the enrichment corresponds to an anthropogenic signal. Salinas beach samples show high concentrations of Fe, V, Cr, Zn, Mn and the highest values of magnetic susceptibility (maximum

  8. Histotripsy Liquefaction of Large Hematomas. (United States)

    Khokhlova, Tatiana D; Monsky, Wayne L; Haider, Yasser A; Maxwell, Adam D; Wang, Yak-Nam; Matula, Thomas J


    Intra- and extra-muscular hematomas result from repetitive injury as well as sharp and blunt limb trauma. The clinical consequences can be serious, including debilitating pain and functional deficit. There are currently no short-term treatment options for large hematomas, only lengthy conservative treatment. The goal of this work was to evaluate the feasibility of a high intensity focused ultrasound (HIFU)-based technique, termed histotripsy, for rapid (within a clinically relevant timeframe of 15-20 min) liquefaction of large volume (up to 20 mL) extra-vascular hematomas for subsequent fine-needle aspiration. Experiments were performed using in vitro extravascular hematoma phantoms-fresh bovine blood poured into 50 mL molds and allowed to clot. The resulting phantoms were treated by boiling histotripsy (BH), cavitation histotripsy (CH) or a combination in a degassed water tank under ultrasound guidance. Two different transducers operating at 1 MHz and 1.5 MHz with f-number = 1 were used. The liquefied lysate was aspirated and analyzed by histology and sized in a Coulter Counter. The peak instantaneous power to achieve BH was lower than (at 1.5 MHz) or equal to (at 1 MHz) that which was required to initiate CH. Under the same exposure duration, BH-induced cavities were one and a half to two times larger than the CH-induced cavities, but the CH-induced cavities were more regularly shaped, facilitating easier aspiration. The lysates contained a small amount of debris larger than 70 μm, and 99% of particulates were smaller than 10 μm. A combination treatment of BH (for initial debulking) and CH (for liquefaction of small residual fragments) yielded 20 mL of lysate within 17.5 minutes of treatment and was found to be most optimal for liquefaction of large extravascular hematomas. Copyright © 2016 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

  9. Report on the Second ARM Mobile Facility (AMF2) Roll, Pitch, and Heave (RPH) Stabilization Platform: Design and Evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Coulter, Richard L. [Argonne National Lab. (ANL), Argonne, IL (United States); Martin, Timothy J. [Argonne National Lab. (ANL), Argonne, IL (United States)


    One of the primary objectives of the U.S. Department of Energy’s Atmospheric Radiation Measurement (ARM) Climate Research Facility’s second Mobile Facility (AMF2) is to obtain reliable measurements of solar, surface, and atmospheric radiation, as well as cloud and atmospheric properties, from ocean-going vessels. To ensure that these climatic measurements are representative and accurate, many AMF2 instrument systems are designed to collect data in a zenith orientation. A pillar of the AMF2 strategy in this effort is the use of a stable platform. The purpose of the platform is to 1) mitigate vessel motion for instruments that require a truly vertical orientation and keep them pointed in the zenith direction, and 2) allow for accurate positioning for viewing or shading of the sensors from direct sunlight. Numerous ARM instruments fall into these categories, but perhaps the most important are the vertically pointing cloud radars, for which vertical motions are a critical parameter. During the design and construction phase of AMF2, an inexpensive stable platform was purchased to perform the stabilization tasks for some of these instruments. The first table compensated for roll, pitch, and yaw (RPY) and was reported upon in a previous technical report (Kafle and Coulter, 2012). Subsequently, a second table was purchased specifically for operation with the Marine W-band cloud radar (MWACR). Computer programs originally developed for RPY were modified to communicate with the new platform controller and with an inertial measurements platform that measures true ship motion components (roll, pitch, yaw, surge, sway, and heave). This platform could not be tested dynamically for RPY because of time constraints requiring its deployment aboard the container ship Horizon Spirit in September 2013. Hence the initial motion tests were conducted on the initial cruise. Subsequent cruises provided additional test results. The platform, as tested, meets all the design and

  10. First cytochemical study of haemocytes from the crab Carcinus aestuarii (Crustacea, Decapoda

    Directory of Open Access Journals (Sweden)

    V. Matozzo


    Full Text Available For the first time, a morphological study of haemocytes from the crab Carcinus aestuarii was carried out by means of light microscopy and differing cytochemical assays. Analysis of haemocyte size frequency distribution (performed by means of a Coulter Counter revealed the presence of two distinct haemocyte fractions in C. aestuarii haemolymph, depending on cell size. The first fraction was of about 3-5 µm in diameter and 30-50 fL in volume, the second was of about 6-12 µm in diameter and over 200 fL in volume. Mean cell diameter and volume were 8.20±1.7 µm and 272.30±143.5 fL, respectively. Haemocytes observed under light microscope were distinguished in three cell types: granulocytes (28%; 11.94±1.43 µm in diameter with evident cytoplasmic granules, semigranulocytes (27%; 12.38±1.76 µm in diameter with less granules than granulocytes, and hyalinocytes (44%; 7.88±1.6 µm in diameter without granules. In addition, a peculiar cell type was occasionally found (about 1%: it was 25-30 µm in diameter and had a great vacuole and a peripheral cytoplasm with granules. Granulocyte and semigranulocyte granules stained in vivo with Neutral Red, indicating that they were lysosomes. Giemsa’s dye confirmed that granulocytes and semigranulocytes were larger than hyalinocytes. Pappenheim’s panoptical staining and Ehrlich’s triacid mixture allowed to distinguish granule-containing cells (including semigranulocytes in acidophils (64%, basophils (35% and neutrophils (1%. Hyalinocytes showed always a basophilic cytoplasm. Haemocytes were positive to the PAS reaction for carbohydrates, even if cytoplasm carbohydrate distribution varied among cell types. Lastly, lipids were found on cell membrane and in cytoplasm of all haemocyte types in the form of black spots produced after Sudan Black B staining. The morphological characterisation of C. aestuarii haemocytes by light microscopy was necessary before performing both ultrastructural and functional

  11. Expanding the functionality and applications of nanopore sensors (United States)

    Venta, Kimberly E.

    Nanopore sensors have developed into powerful tools for single-molecule studies since their inception two decades ago. Nanopore sensors function as nanoscale Coulter counters, by monitoring ionic current modulations as particles pass through a nanopore. While nanopore sensors can be used to study any nanoscale particle, their most notable application is as a low cost, fast alternative to current DNA sequencing technologies. In recent years, signifcant progress has been made toward the goal of nanopore-based DNA sequencing, which requires an ambitious combination of a low-noise and high-bandwidth nanopore measurement system and spatial resolution. In this dissertation, nanopore sensors in thin membranes are developed to improve dimensional resolution, and these membranes are used in parallel with a high-bandwidth amplfier. Using this nanopore sensor system, the signals of three DNA homopolymers are differentiated for the first time in solid-state nanopores. The nanopore noise is also reduced through the addition of a layer of SU8, a spin-on polymer, to the supporting chip structure. By increasing the temporal and spatial resolution of nanopore sensors, studies of shorter molecules are now possible. Nanopore sensors are beginning to be used for the study and characterization of nanoparticles. Nanoparticles have found many uses from biomedical imaging to next-generation solar cells. However, further insights into the formation and characterization of nanoparticles would aid in developing improved synthesis methods leading to more effective and customizable nanoparticles. This dissertation presents two methods of employing nanopore sensors to benet nanoparticle characterization and fabrication. Nanopores were used to study the formation of individual nanoparticles and serve as nanoparticle growth templates that could be exploited to create custom nanoparticle arrays. Additionally, nanopore sensors were used to characterize the surface charge density of anisotropic

  12. Interstitial diffuse radiance spectroscopy of gold nanocages and nanorods in bulk muscle tissues

    Directory of Open Access Journals (Sweden)

    Grabtchak S


    Full Text Available Serge Grabtchak,1,2 Logan G Montgomery,1 Bo Pang,3,4 Yi Wang,4,5 Chao Zhang,6,7 Zhiyuan Li,6,7 Younan Xia,4,8 William M Whelan1,91Department of Physics, University of Prince Edward Island, Charlottetown, PEI, Canada; 2Departments of Electrical and Computer Engineering, and Physics, Dalhousie University, Halifax, Canada; 3Department of Biomedical Engineering, Peking University, Beijing, People’s Republic of China; 4The Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA; 5Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing, People’s Republic of China; 6Laboratory of Optical Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, People’s Republic of China; 7College of Physics and Optoelectronics, South China University of Technology, Guangzhou, People’s Republic of China; 8School of Chemistry and Biochemistry, and School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, USA; 9Atlantic Veterinary College, Charlottetown, PEI, CanadaAbstract: Radiance spectroscopy was applied to the interstitial detection of localized inclusions containing Au nanocages or nanorods with various concentrations embedded in porcine muscle phantoms. The radiance was quantified using a perturbation approach, which enabled the separation of contributions from the porcine phantom and the localized inclusion, with the inclusion serving as a perturbation probe of photon distributions in the turbid medium. Positioning the inclusion at various places in the phantom allowed for tracking of photons that originated from a light source, passed through the inclusion’s location, and reached a detector. The inclusions with high extinction coefficients were able to absorb nearly all photons in the range of 650–900 nm, leading to a spectrally flat radiance signal. This signal could be

  13. Proceedings of Patient Reported Outcome Measure’s (PROMs Conference Sheffield 2016: advances in patient reported outcomes research

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    Tim Croudace


    longitudinal parameter shifts in epidemiological data: measurement invariance and response shifts in cohort and survey data describing the UK’s Quality of Life Jan R. Boehnke O12 Patient-reported outcomes within health technology decision making: current status and implications for future policy Andrew Trigg, Ruth Howells O13 Can social care needs and well-being be explained by the EQ-5D? Analysis of Health Survey for England dataset Jeshika Singh, Subhash Pokhrel, Louise Longworth O14 Where patients and policy meet: exploring individual-level use of the Long-Term Conditions Questionnaire (LTCQ Caroline Potter, Cheryl Hunter, Laura Kelly, Elizabeth Gibbons, Julian Forder, Angela Coulter, Ray Fitzpatrick, Michele Peters

  14. Benzene activates caspase-4 and -12 at the transcription level, without an association with apoptosis, in mouse bone marrow cells lacking the p53 gene

    Energy Technology Data Exchange (ETDEWEB)

    Yi, Jung-Yeon; Han, Jeong-Hee; Yoon, Byung-Il [Kangwon National University, School of Veterinary Medicine, Chuncheon, Gangwon (Korea); Hirabayashi, Yoko; Kodama, Yukio; Kanno, Jun [National Institute of Health Sciences, Division of Cellular and Molecular Toxicology, Center for Biological Safety and Research, Tokyo (Japan); Choi, Yang-Kyu [Konkuk University, College of Veterinary Medicine, Seoul (Korea); Inoue, Tohru [National Institute of Health Sciences, Biological Safety and Research Center, Tokyo (Japan)


    Benzene is a well-known environmental pollutant that can induce hematotoxicity, aplastic anemia, acute myelogenous leukemia, and lymphoma. However, although benzene metabolites are known to induce oxidative stress and disrupt the cell cycle, the mechanism underlying lympho/leukemogenicity is not fully understood. Caspase-4 (alias caspase-11) and -12 are inflammatory caspases implicated in inflammation and endoplasmic reticulum stress-induced apoptosis. The objectives of this study were to investigate the altered expression of caspase-4 and -12 in mouse bone marrow after benzene exposure and to determine whether their alterations are associated with benzene-induced bone marrow toxicity, especially cellular apoptosis. In addition, we evaluated whether the p53 gene is involved in regulating the mechanism, using both wild-type (WT) mice and mice lacking the p53 gene. For this study, 8-week-old C57BL/6 mice [WT and p53 knockout (KO)] were administered a benzene solution (150 mg/kg diluted in corn oil) via oral gavage once daily, 5 days/week, for 1 or 2 weeks. Blood and bone marrow cells were collected and cell counts were measured using a Coulter counter. Total mRNA and protein extracts were prepared from the harvested bone marrow cells. Then qRT-PCR and Western blotting were performed to detect changes in the caspases at the mRNA and protein level, respectively. A DNA fragmentation assay and Annexin-V staining were carried out on the bone marrow cells to detect apoptosis. Results indicated that when compared to the control, leukocyte number and bone marrow cellularity decreased significantly in WT mice. The expression of caspase-4 and -12 mRNA increased significantly after 12 days of benzene treatment in the bone marrow cells of benzene-exposed p53KO mice. However, apoptosis detection assays indicated no evidence of apoptosis in p53KO or WT mice. In addition, no changes of other apoptosis-related caspases, such as caspase-3 and -9, were found in WT or p53KO mice at the

  15. Polymerization time for a microwave-cured acrylic resin with multiple flasks Tempo de polimerização de resina acrílica em microondas, utilizando múltiplas muflas

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    Daniela Maffei Botega


    Full Text Available This study aimed at establishing the polymerization time of a microwave-cured acrylic resin (AcronTM MC, simultaneously processing 2, 4, and 6 flasks. Required time was measured according to the parameters: monomer release in water, Knoop hardness, and porosity. Samples were made with AcronTM MC in different shapes: rectangular (32 x 10 x 2.5 mm for monomer release and porosity; and half-disc (30 mm in diameter x 4 mm in height for Knoop hardness. There were four experimental groups (n = 24 per group: G1 one flask (control; G2 two flasks; G3 four flasks, and G4 six flasks. At first, polymerization protocol was similar for all groups (3 min/450 W. Time was then adjusted for G2, G3, and G4, based on monomer release evaluation in the control group, obtained by spectrophotometer Beckman DU-70, with emitting wave of 206 nm. Knoop hardness test was performed using a Shimadzu HMV 2000 hardness tester, and 10 indentations were performed on each specimen's surface. Porosity was assessed after specimens were immersed in black ink and the pores counted in a microscope. Results showed that the complete polymerization of the resin occurred in 4.5 min for two flasks (G2; 8.5 min for four flasks (G3; and 13 min for six flasks (G4, all with 450 W. Statistical analysis revealed that the number of flasks does not interfere with polymerization, Knoop hardness, and porosity of the resin. Results showed that polymerization of microwave-curing resin with more than one flask is a viable procedure, as long as polymerization time is adjusted.O objetivo deste estudo foi o de determinar os tempos necessários para a polimerização padrão de uma resina acrílica em microondas, utilizando várias muflas simultaneamente. Os tempos necessários foram aferidos por parâmetros como monômeros liberados em água, dureza Knoop e porosidade. As amostras, confeccionadas em resina AcronTM MC, apresentavam as seguintes dimensões: para os parâmetros monômero residual e porosidade

  16. Atributos químicos de um latossolo bruno sob sistema plantio direto em função da estratégia de adubação e do método de amostragem de solo Chemical properties of a haplohumox under no-tillage as related to fertilization strategies and soil sampling method

    Directory of Open Access Journals (Sweden)

    Volnei Pauletti


    greater in the no-tillage system because there is no homogenization of the soil surface layer. This study evaluated the effect of fertilization strategies on soil chemical properties at distinct soil depths after three and six years from the beginning of the experiment in a Haplohumox under long-term no-tillage. Besides, the variation of these properties considering two methods of soil sampling, with and without inclusion of the seeding row, was evaluated. Ten treatments were applied, nine considering P sources (rock phosphate and triple superphosphate, row preparation equipment (double disc opener and coulter opener, fertilizer placement (in-row and broadcast, time of application (summer and winter and one control treatment, without fertilizer. The measured soil chemical properties were higher in the surface layers and depth distribution, except for P, was not influenced by the fertilization strategies. The soil P values were the lowest in the 0-5 cm layer where the coulter opener equipment had been continuously used for more than three years and no fertilizer was applied. With the inclusion of the sowing row in the soil sampling, higher K, Ca and CEC values were observed. The methodologies of soil sampling, with and without the inclusion of the sowing row and the 0-10 and 0-20 cm sampling layers resulted in similar fertilizer and lime recommendations for areas under long-term no-tillage.

  17. Association of single nucleotide polymorphism at position 45 in adiponectin gene with plasma adiponectin level and insulin resistance in obesity

    International Nuclear Information System (INIS)

    Chen Xiaoyu; Li Xisheng; Lin Xiahong; Gao Hongzhi; Li Qiulan; Zha Jinshun


    Objective: To explore the association of single nucleotide polymorphism at position 45 (SNP45) in adiponectin gene with plasma adiponectin level and insulin resistance in obesity in Quanzhou area of Fujian province. Methods: Two hundred and forty-eight patients with obesity and 225 normal control subjects were enrolled in this study.Fasting insulin (FINS) were measured by radioimmunoassay and fasting plasma glucose (FPG), total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C) were measured by BECKMAN DXC800 biochemistry analyzer. Body mass index (BMI), waist to hip ratio,homeostasis model assessment of insulin resistance (HOMA-IR) were calculated. Plasma adiponectin levels were examined by means of enzyme-linked immunosorbentassy. The adiponectin gene SNP45 was identified by PCR-restriction fragment length polymorphism. Results: (1) Frequencies of GG+GT genotype in obesity group and normal control group were 61% and 44% respectively (χ 2 =14.182, P<0.01), and G allele frequencies were 35% and 25% (χ 2 =10.708, P<0.01). (2) In obesity group,the subjects with SNP45 GG+GT genotype had higher TG and LDL-C levels than those with TT genotype (t=2.604, P<0.01; t=5.507, P<0.01), and had lower adiponectin level than those with TT genotype (t=2.275, P<0.05), and had significantly lower HDL-L level than those with TT genotype (t=10.100, P< 0.01). (3) In normal control group,the subjects with SNP45 GG +GT genotype had significantly lower adiponectin,TG,TC levels than those with TT genotype (t=2.510, P<0.05; t=2.922, P<0.01; t=3.272, P< 0.01). (4) Logistic analysis proved that the SNP45 GG+GT genotype in obesity group was associated with decreased risk of plasma adiponectin level (OR=0.810, 95% CI : 0.673-0.975, P<0.05), and with increased risk of HOMA-IR (OR=1.746, 95% CI : 1.060-2.875, P<0.05). The SNP45 GG+GT genotype in normal control group was associated with increased risk of HOMA-IR (OR=3

  18. Infrared Absorption by Atmospheric Aerosols in Mexico City during MILAGRO. (United States)

    Kelley, K. L.; Mangu, A.; Gaffney, J. S.; Marley, N. A.


    Past research in our group using cylindrical internal reflectance spectroscopy has indicated that aqueous aerosols could contribute to the radiative warming as greenhouse species (1,2). Although aerosol radiative effects have been known for sometime and are considered one of the major uncertainties in climate change modeling, most of the studies have focused on the forcing due to scattering and absorption of radiation in the uv- visible region (3). Infrared spectral information also allows the confirmation of key functional groups that are responsible for enhanced absorption observations from secondary organics in the uv-visible region. This work extends our efforts to evaluate the infrared absorption by aerosols, particularly organics, that are now found to be a major fraction of urban and regional aerosols in the 0.1 to 1.0 micron size range and to help identify key types of organics that can contribute to aerosol absorption. During the MILAGRO campaign, quartz filter samples were taken at 12-hour intervals from 5 am to 5 pm (day) and from 5 pm to 5 am (night) during the month of March 2006. These samples were taken at the two super-sites, T-0 (Instituto Mexicano de Petroleo in Mexico City) and T-1 (Universidad Technologica de Tecamac, State of Mexico). The samples have been characterized for total carbon content (stable isotope mass spectroscopy) and natural radionuclide tracers, as well as for their UV-visible spectroscopic properties by using integrating sphere diffuse reflectance spectroscopy (Beckman DU with a Labsphere accessory). These same samples have been characterized in the mid and near infrared spectral ranges using diffuse reflection spectroscopy (Nicolet 6700 FTIR with a Smart Collector accessory). Aerosol samples were removed from the surfaces of the aerosol filters by using Si-Carb sampler. The samples clearly indicate the presence of carbonyl organic constituents and the spectra are quite similar to those observed for humic and fulvic acids

  19. Enhanced UV Absorption in Carbonaceous Aerosols during MILAGRO and Identification of Potential Organic Contributors. (United States)

    Mangu, A.; Kelley, K. L.; Marchany-Rivera, A.; Kilaparty, S.; Gunawan, G.; Gaffney, J. S.; Marley, N. A.


    Measurements of aerosol absorption were obtained as part of the MAX-Mex component of the MILAGRO field campaign at site T0 (Instituto Mexicano de Petroleo in Mexico City) during the month of March, 2006 by using a 7- channel aethalometer (Thermo-Anderson). These measurements, obtained at 370, 470, 520, 590, 660, 880, and 950 nm at a 5 minute time resolution, showed an enhanced absorption in the UV over that expected from carbon soot alone. Samples of fine atmospheric aerosols (less than 0.1micron) were also collected at site T0 and T1 (Universidad Technologica de Tecamac, State of Mexico) from 5 am to 5 pm (day) and from 5 pm to 5 am (night) during the month of March 2006. The samples were collected on quartz fiber filters with high volume impactor samplers. The samples have been characterized for total carbon content (stable isotope ratio mass spectroscopy) and natural radionuclide tracers (210Pb, 210Po, 210Bi, 7Be, 13C, 14C, 40K, 15N). Continuous absorption spectra of these aerosol samples have been obtained in the laboratory from 280 to 900nm with the use of an integrating sphere coupled to a UV-visible spectrometer (Beckman DU with a Labsphere accessory). The integrating sphere allows the detector to collect and spatially integrate the total radiant flux reflected from the sample and therefore allows for the measurement of absorption on highly reflective or diffusely scattering samples (1). The continuous spectra also show an enhanced UV absorption over that expected from carbon soot and the general profiles are quite similar to those observed for humic and fulvic acids found as colloidal materials in surface and groundwaters (2), indicating the presence of humic-like substances (HULIS) in the fine aerosols. The spectra also show evidence of narrow band absorbers below 400 nm typical of polycyclic aromatics (PAH) and nitrated aromatic compounds. Spectra were also obtained on NIST standard diesel soot (SRM 2975), NIST standard air particulate matter (SRM 8785

  20. The Argonne Leadership Computing Facility 2010 annual report.

    Energy Technology Data Exchange (ETDEWEB)

    Drugan, C. (LCF)


    that will be faster than petascale-class computers by a factor of a thousand. Pete Beckman, who served as the ALCF's Director for the past few years, has been named director of the newly created Exascale Technology and Computing Institute (ETCi). The institute will focus on developing exascale computing to extend scientific discovery and solve critical science and engineering problems. Just as Pete's leadership propelled the ALCF to great success, we know that that ETCi will benefit immensely from his expertise and experience. Without question, the future of supercomputing is certainly in good hands. I would like to thank Pete for all his effort over the past two years, during which he oversaw the establishing of ALCF2, the deployment of the Magellan project, increases in utilization, availability, and number of projects using ALCF1. He managed the rapid growth of ALCF staff and made the facility what it is today. All the staff and users are better for Pete's efforts.

  1. Implications of Climate Volatility for Agricultural Commodity Markets in the Presence of Biofuel Mandates (United States)

    Verma, M.; Diffenbaugh, N. S.; Hertel, T. W.; Beckman, J.


    In presence of bio-fuels, link between energy and agricultural commodity markets has become more complex. An increase in ethanol production to minimum 15bn gallons a year - Renewable Fuel Standard (RFS) and current technically permissible maximum 10% blending limit - Blend Wall (BW); make the link even stronger. If oil prices in future do not rise significantly from their current levels, this minimum production requirement would likely be binding. In such a scenario any fluctuation in crop production will have to be absorbed by the non-ethanol usage of the crop and would translate into crop prices adjusting to clear the markets and therefore the commodity prices will be more volatile. At high oil prices it is possible that the BW may become binding, severing the link between oil prices and commodity prices as well, potentially leading to higher price volatility. Hertel and Beckman (2010) find that, with both RFS and BW simultaneously binding, corn price volatility due to supply side shocks (which could arise from extreme climate events) could be more than 50% as large as in the absence of bio-fuel policies. So energy markets are important determinants of agricultural commodity price volatility. This proposal intends to introduce the increased supply side volatility on account of climate change and volatility, in the framework. Global warming on account of increased GHG concentrations is expected to increase the intensity and frequency of hot extremes in US (Diffenbaugh et al. 2008) and therefore affect corn yields. With supply shocks expected to increase, binding RFS and BW will exacerbate the volatility, while if they are non-binding then the price changes could be cushioned. We propose to model the impacts of climate changes and volatility on commodity prices by linking three main components - a. Projections for change in temperature and precipitation using climate model b. A statistical model to predict impacts of change in climate variable on corn yields in US

  2. P-gp Expression and Rh 123 Efflux Assay Have no Impact on Survival in Egyptian Pediatric Acute Lymphoblastic Leukemia Patients

    International Nuclear Information System (INIS)

    Kamel, A.M.; El-Sharkawy, N.; Yassin, D.; Shaaban, Kh.; Hussein, H.; Sidhom, I; Abo El-Naga, S.; Ameen, M.; El-Hattab, O.; Aly El-Din, N.H.


    In a previous work we have studied MDR status in terms of P-glycoprotein (P-gp) expression and Rhodamine 123 efflux assay in Egyptian acute leukemia patients. We have reported results comparable to the literature as regards ANLL both in pediatric and adult cases. However, higher figures were encountered for the functional assay in ALL. As our ALL cases especially in pediatric age group show worse prognosis compared to literature, we hypothesized that the higher percentage of cases with positive Rh 123 efflux assay might be a contributing factor. Material and Methods: A total of 108 cases were studied including 80 ALL and 28 ANLL. ALL cases included 48 male and 32 female with an age range of 6m to 18 yrs and a median of 7 yrs. ANLL cases included 18 male and 10 female with an age range of 6m to 18 yrs and a median of 8 yrs. P-gp expression was evaluated using 4E3 and DIC2 mAb, analyzed by Coulter XL flow cytometer and expressed as a ratio at a cut off of ≤1.1 and/or ≤5% positive cells. For the evaluation of MDR function Rh 123 efflux assay using cyclosporine as a blocker and expressed as a ratio at a cutoff of ≤1. 1 and/or ≤ 10% positive cells was performed. MDR expression and function were correlated to age, Hb, TLC, CD34 expression, immuno phenotype and DNA index in ALL, FAB subtypes in ANLL as well as to CR, DFS and EFS in ALL. In ALL, P-gp expression was encountered in 26.4% of cases. Positive Rh efflux was reported in 61.5%. No correlation was encountered between neither expression nor functional assay with age, Hb, TLC, CD34 expression or immuno phenotype. CR was achieved in 89.8%; neither P-gp expression nor Rh123 efflux had an impact on CR except for Rh 123 efflux in T-ALL where a cutoff of 1.25 could predict CR at a total accuracy of 70.6%. DFS was 92.3% while EFS was 72.2% for the whole group. No significant difference was encountered neither between cases expressing or lacking P-gp nor between cases with negative or positive Rh 123 efflux assay

  3. Essai de classement typo-technologique des araires à partir des pièces métalliques découvertes en Gaule romaine en vue de leur reconstitution Setting up of a typological-technological classification method to try to reconstruct ards from metallic pieces found in Roman Gaul

    Directory of Open Access Journals (Sweden)

    André Marbach


    positions? A method of research has been developed from a fine analysis of the metallic pieces listed in a catalogue of these pieces for Gaul. The shares have been classified according to their working surface, the form of their sockets and the angle of the wearing surface with the ground if it is observable. As for the tanged shares the filing is similar, the length of the tang has however been taken into account here. From the values known for these pieces and a technical study with modelisation of the tilling implement, the possibility of a reconstruction of the ard has been explored. Researchers are here reminded of how important it is to publish the description of the pieces found with the utmost precision for the drawing as well as for the dimensions. As far as ards fitted with a socket share are concerned uncertainty prevails except for shares with a small working surface which, generally, are single ards (manche-sep. The reconstruction of tanged share-ards seems possible including the length of the draught-beam (chambige. Several concrete examples of reconstructed ards have been studied. Thus, if the reconstruction of a tanged share from metallic pieces, tang and coulter may be envisaged, that of iron share ards is uncertain except for plough-ards (reversible.

  4. The pyrethroid metabolites 3-phenoxybenzoic acid and 3-phenoxybenzyl alcohol do not exhibit estrogenic activity in the MCF-7 human breast carcinoma cell line or Sprague-Dawley rats

    International Nuclear Information System (INIS)

    Laffin, Brian; Chavez, Marco; Pine, Michelle


    Synthetic pyrethroids are one of the most frequently and widely used class of insecticides, primarily because they have a higher insect to mammalian toxicity ratio than organochlorines or organophosphates. The basic structure of pyrethroids can be characterized as an acid joined to an alcohol by an ester bond. Pyrethroid degradation occurs through either oxidation at one or more sites located in the alcohol or acid moieties or hydrolysis at the central ester bond, the latter reaction being important for mammalian metabolism of most pyrethroids. The primary alcohol liberated from the ester cleavage is hydroxylated to 3-phenoxybenzyl alcohol, which for most pyrethroids is then oxidized to 3-phenoxybenzoic acid. These products may then be conjugated with amino acids, sulfates, sugars, or sugar acids. In vitro studies have suggested that some of the pyrethroids may have estrogenic activity. Interestingly, the chemical structure of specific pyrethroid metabolites indicates that they may be more likely to interact with the estrogen receptor than the parent compounds. Two of the pyrethroid metabolites, 3-phenoxybenzoic acid (3PBA) and 3-phenoxybenzyl alcohol (3PBalc) have been reported to have endocrine activity using a yeast based assay. 3PBAlc exhibited estrogenic activity with reported EC 50 s of 6.67 x 10 -6 and 2 x 10 -5 while 3PBAcid exhibited anti-estrogenic activity with a calculated IC 50 of 6.5 x 10 -5 . To determine if the metabolites were able to cause the same effects in a mammalian system, the estrogen-dependent cell line, MCF-7, was utilized. Cells were treated with 1.0, 10.0 or 100.0 μM concentrations of each metabolite and cytotoxicity was assessed. The two lowest concentrations of both metabolites did not induce cell death and even appeared to increase proliferation over that of the control cells. However, when cellular proliferation was measured using a Coulter counter neither metabolite stimulated proliferation (1.0 nM, 10.0 nM, or 10.0 μM) or

  5. Desempenho de uma semeadora-adubadora direta, em função da velocidade de eslocamento e do tipo de manejo da biomassa da cultura de cobertura do solo Performance of a seeding-fertilizer machine in function of the displacement speed and soil cover crop biomass management

    Directory of Open Access Journals (Sweden)

    Klinger B. Branquinho


    Full Text Available O objetivo do presente trabalho foi estudar o desempenho de uma semeadora-adubadora, operando em duas velocidades sobre a palhada da cultura do milheto (Pennisetum glaucum (L. R. Brown, após diferentes manejos, na semeadura direta da cultura da soja (Glycine Max L.. O experimento foi realizado com seis tratamentos, em esquema fatorial, delineados em blocos ao acaso, com quatro repetições. Os tratamentos combinaram três manejos (triturador de palhas tratorizado, rolo- faca e herbicida com duas velocidades de deslocamento da semeadora-adubadora (5,2 e 7,3 km h-1. Foram analisadas as seguintes variáveis: acúmulo e decomposição de massa seca do milheto, índice de emergência da cultura da soja, uniformidade de distribuição longitudinal de sementes, capacidade de campo efetiva da semeadora-adubadora e produtividade da soja. Os resultados mostraram que não houve diferença na decomposição da massa de milheto após os manejos e que esses, juntamente com a velocidade de deslocamento do conjunto trator-semeadora-adubadora, não influenciaram no número de dias para a emergência das plântulas de soja e na distribuição longitudinal das sementes. A capacidade de campo efetiva da semeadora-adubadora foi maior na velocidade mais alta. O rendimento de grãos não diferiu significativamente nos tratamentos estudados.The objective of the present work was to evaluate three different managements of the Pennisetum glaucum (L. R. Brown crop and two-speed displacement in a no-tillage system of soybean (Glycine max L. crop on a typical Eutrophic Red Latosol. The experiment was arranged in six treatments, in a factorial array, in a randomized complete block design, with four replications. The treatments were constituted from three combination of crop managements (straw chopper, residue coulter and herbicide and two speed displacement of the seeding-fertilizer machine (5.2 and 7.3 km h-1. The following variables have been analyzed: emergency index of

  6. Resistência mecânica do solo e força de tração em hastes sulcadoras de semeadoras-adubadoras em sistema de integração lavoura-pecuária Mechanical resistance of soil and traction force by planter chisel type openers in crop-livestock system

    Directory of Open Access Journals (Sweden)

    Osmar Conte


    . Treatments consisted of four grazing intensities, which resulted in pasture heights of 10; 20; 30 and 40 cm, and a no-grazed area used as blank, designed in randomized blocks with three replicates. After the grazing period and during soybean sowing, RP was evaluated; also, required power to openers chisel type coulter was measured. Both parameters increased with increments in grazing intensity. There was correlation between both parameters, showing that it is possible to evaluate the condition of soil´s compaction by measuring draft requirement by fertilizer furrow openers.

  7. The subglacial Lake Vostok (East Antarctica) surface snow is Earth-bound DNA (and dust)-free (United States)

    Bulat, S.; Marie, D.; Bulat, E.; Alekhina, I.; Petit, J.-R.


    The objective was to assess the microbial cell abundance in the surface snow in Central East Antarctica and the fate of microbial genomic DNA during summer short-time exposure to surface climatic (and radiation) conditions at Vostok using flow cytometry and DNA-based methods. The surface snow (until 4m deep) was collected as clean as possible in the vicinity of the Vostok station (3 sites - courtesy of A Ekaykin and ASC Lebedev Physical Iinstitute RAS) and towards the Progress station (4 more sites with one just 29km from the coast - courtesy of A Ekaykin and S Popov) in specially decontaminated plastic crates or containers of various volumes (up to 75 kg of snow). All subsequent snow treatment manipulations (melting, concentrating, genomic DNA extraction, primary PCR set up) were performed in clean room laboratory facilities (LGGE, UJF-CNRS, Grenoble, France). Cell concentrations were determined on meltwater aliquots prepared under clean room conditions using flow cytofluorometry (Biostation, Roscoff, France). The highly concentrated meltwater (until 10000 times down) was used to extract gDNA which were subjected to bacterial 16S rRNA genes amplification in PCR and sequencing. The gDNA of a complex mesophile microbial community for exposure trials were also prepared and put onto a filter under strict clean room conditions. The filters were got exposed open to solar radiation and surface temperature at Vostok during January for various time duration periods (from 25 to 1 day). As a result no microbial cells were confidently detected in surface snow samples differed by sampling sites and people asked to collect as well. Complementary the mineral dust particle abundance did not exceed 16 mkg per liter with the particle size mode about 2.5 mkm as shown using Coulter counter. Preliminary amongst the microparticles no unusual findings (e.g. spherules of cosmic origin) were observed by shape and element composition using electron scanning microscopy. The gDNA studies

  8. Relation of sortable silt grain-size to deep-sea current speeds: Calibration of the 'Mud Current Meter' (United States)

    McCave, I. N.; Thornalley, D. J. R.; Hall, I. R.


    Fine grain-size parameters have been used for inference of palaeoflow speeds of near-bottom currents in the deep-sea. The basic idea stems from observations of varying sediment size parameters on a continental margin with a gradient from slower flow speeds at shallower depths to faster at deeper. In the deep-sea, size-sorting occurs during deposition after benthic storm resuspension events. At flow speeds below 10-15 cm s-1 mean grain-size in the terrigenous non-cohesive 'sortable silt' range (denoted by SS bar , mean of 10-63 μm) is controlled by selective deposition, whereas above that range removal of finer material by winnowing is also argued to play a role. A calibration of the SS bar grain-size flow speed proxy based on sediment samples taken adjacent to sites of long-term current meters set within 100 m of the sea bed for more than a year is presented here. Grain-size has been measured by either Sedigraph or Coulter Counter, in some cases both, between which there is an excellent correlation for SS bar (r = 0.96). Size-speed data indicate calibration relationships with an overall sensitivity of 1.36 ± 0.19 cm s-1/μm. A calibration line comprising 12 points including 9 from the Iceland overflow region is well defined, but at least two other smaller groups (Weddell/Scotia Sea and NW Atlantic continental rise/Rockall Trough) are fitted by sub-parallel lines with a smaller constant. This suggests a possible influence of the calibre of material supplied to the site of deposition (not the initial source supply) which, if depleted in very coarse silt (31-63 μm), would limit SS bar to smaller values for a given speed than with a broader size-spectrum supply. Local calibrations, or a core-top grain-size and local flow speed, are thus necessary to infer absolute speeds from grain-size. The trend of the calibrations diverges markedly from the slope of experimental critical erosion and deposition flow speeds versus grain-size, making it unlikely that the SS bar (or

  9. Analysis of changes in the percentage of B (CD19) and T (CD3) lymphocytes, nk cells, subsets CD4, CD8 in differentiated thyroid cancer patients treated with iodine-131

    International Nuclear Information System (INIS)

    Luo Quanyong; Yu Yongli; Chen Libo; Lu Hankui; Zhu Ruisen


    Objective: To evaluate the changes in the percentage of B (CD19) and T (CD3) lymphocytes, NK cells, subsets CD4, CD8 in patients with differentiated thyroid carcinoma (DTC) who received iodine-131 for therapeutic purposes. Methods: In this study, 102 DTC patients were divided into three groups. Group A, 8 cases received 1850 MBq of iodine-131 for the remnant thyroid ablation. Group B, 43 cases received 3700 MBq of iodine-131 for the treatment of cervical lymph node metastasis. Group C, 51 cases received 7400 MBq of iodine-131 for remote metastasis. All patients were in a hypothyroid state at the time of administration of iodine-131 and resumed L-thyroxine (2μg/Kg/day) 5 days after iodine-131 administration. The percentage of B and T lymphocytes, NK cells, subsets CD4, CD8 in peripheral blood were serially analyzed at baseline and at days 7, 30 and 90 after iodine-131 administration using a Coulter EPICS XL cytometer. Ten healthy individuals were used as a control group for lymphocyte subset values. Results: Comparing the basal lymphocyte subset levels in groups A, B and C with the control group, only NK cells showed significantly higher levels in patients than in controls (P=0.043). In group A, only the percentage of NK cells (P=0.031) and B cells (P =0.024) were reduced at day 7. In group B, a decrease in the percentage of NK cells at days 7(P=0.005), 30 (P=0.021) was observed, while a significant decrease in the percentage of B cells was only observed at day 7(P=0.006). Among T cells, only CD4+ was obviously affected, resulting in a reduction in the CD4+/CD8+ ratio at day 30 (P=0.034). In group C, patients showed a decrease in the percentage of NK cells at days 7 (P=0.023), 30 (P=0.006). A decrease in the percentage of both B and T lymphocytes was observed at days 7(P=0.020, 0.018 respectively), 30(P=0.041, 0.025 respectively). Among T cells, a decrease in the percentage of CD4+ and an increase in the percentage of CD8+ were observed, resulting in a marked

  10. A special year, thanks and a look ahead (United States)

    Birch, David J. S.


    Dear authors, reviewers and readers of Measurement Science and Technology , I would like to thank all those who have published papers with us in 2013, and offer a special thanks to those of you who have given up much of your precious time and kindly reviewed articles for the journal. 2013 was of course a very special year for the journal, marking the 90th anniversary of the journal's foundation, as Journal of Scientific Instruments. In order to mark the anniversary we started the year with a historical perspective of the journal [1], and a historical review of 90 years of dielectric properties measurement of materials [2]. The celebrations continued with a stimulating meeting at the Institute of Physics in London in March, entitled 'Frontiers of Measurement'. In keeping with the multidisciplinary ethos of the journal, the speakers covered a diverse range of topics, all linked by the common language of measurement science. Patrick Gill of NPL talked about atomic clocks, Bruce Tromberg of the Beckman Laser Institute and Medical Clinic covered biophotonics, Roger Bisby of the University of Salford lectured on developments in biochemical techniques, Ivan Marusic of the University of Melbourne spoke on fluids and Mervyn Miles of the University of Bristol described recent developments in atomic force microscopy. Nicola Gulley, Editorial Director of IOP Publishing (IOPP), briefed the meeting on some of the latest developments in the world of scientific publishing. The meeting ended with a presentation to Sharon D'Souza, outgoing Publisher of the journal, and a welcome for Ian Forbes who has taken over the role. On behalf of all the Editorial Board I would like to offer a very special thanks to Sharon for all her great work for the journal over many years and wish her every success in her new IOPP posts as Publisher of Reports in Progress in Physics and the recently launched Methods and Applications in Fluorescence. Ian Forbes comes to the journal with a PhD in Physics

  11. Phase 1 Final Technical Report - MgB2 Synthesis: Pushing to High Field Performance

    International Nuclear Information System (INIS)

    Bhatia, Mohit; McIntyre, Peter


    crystalline boron results in the formation of parasitic phases such as MgB4, MgB7, etc. Such parasitic phases are a primary element of the connectivity problem, in which even though a sample powder may contain grains of high-quality MgB2, adjacent grains are surrounded by intergrowths of parasitic phases so that current trans-port is badly degraded. The best results to date have been obtained using boron powder produced long ago for a rocket propellant development project. The synthesis process was complex and is now largely lost, and the manufacturing equipment has long since been scrapped. The last batch of the powder has been used during recent years to support MgB2 R and D at several labs, but supplies are dwindling. ATC has identified a first application of its plasma torch to synthesize phase-pure amorphous boron flake using a rapid-quench splat technique. Inexpensive technical-grade boron would be purified of contaminants, then dispersed as an aerosol in inert gas and passed through the plasma torch to melt it into a spray. The spray would be splat-condensed on a rotating drum to form pure amorphous flake. The process would begin with technical-grade boron powder, having good stoichiometric purity, nanoscale particles, but significant contamination of MgO and crystalline boron. We used wet chemistry to remove B2O3 completely and reduced the MgO impurity, and analyzed the particle size distribution using a Coulter counter and the phase composition using X-ray diffrac-tion (XRD). The next step will be to build an rf plasma torch with a recirculating single-component aerosol feed and the cooled splat drum and collector, and undertake process devel-opment for amorphous boron powder. This revised goal has two benefits. First, it is an easier technology than our ultimate goal of a multi-component laminar flow torch. We have been counseled by those experienced in plasma torch technology that our ultimate goal will require a torch that should be feasible but has never been

  12. Preparo do solo em áreas de produção de grãos, silagem e pastejo: efeito na resistência tênsil e friabilidade de agregados Soil tillage in grain and silage-producing areas and pasture: effect on tensile strength and aggregate friability

    Directory of Open Access Journals (Sweden)

    Marina Araújo Bavoso


    ção da F. Os resultados demonstraram que a RT foi influenciada pelos sistemas de produção e tipos de preparo do solo. O menor valor de RT foi verificado no solo sob Pastejo, enquanto o Sistema Plantio Direto apresentou o maior valor de RT. A friabilidade não distinguiu os sistemas de produção e tipos de preparo de solo estudados.The understanding and quantification of soil use and management on the soil physical quality are important to develop and select sustainable agricultural systems. The purpose of this study was to evaluate the structural quality of a clayey Brazilian Oxisol under different production and soil tillage systems by measuring the tensile strength (TS and to quantify aggregate friability (F. The study was conducted in Castro, Paraná state, in southern Brazil, using three production systems and seven soil tillage types, in a randomized block design. The following production systems were evaluated: (PS I - ryegrass for soil cover (winter and corn for grain production (summer; (PS II ryegrass for haylage/silage and corn for grain production; and (PS III - ryegrass for grazing and corn for grain production. Seven types of soil tillage were evaluated in each system: (G1 - plow harrow in winter, depth 0.15 m; (G2 - plow harrow in winter and summer, depth 0.15 m; (Plow - disk plow, depth 0.20 m; (Laser wing subsoiler - subsoiler wing tips, to a depth of 0.45 m; (Subsoiler - subsoiler, depth 0.80 m; (Aerator - soil aerater Aeromix®; and (NT - No-tillage, where ryegrass was sown using a double disc and corn with a coulter. From each experimental plot soil blocks (0.20 x 0.15 x 0.07 m were taken which were broken up by hand in their natural aggregates. Then, the aggregates were air-dried for 24 h and passed through sieves with diameters of 12.5 and 19 mm. Forty aggregates from each block were selected and tested for indirect tension to determine TS and quantify F. TS was influenced by the production systems and soil tillage types. The lowest and the

  13. Increasing energy efficiency by geometric modification of hoe-type furrow opener

    Directory of Open Access Journals (Sweden)

    R Rahimzadeh


    Full Text Available Introduction Direct planting becomes more common in the recent years, because it conserves soil and water as well as it saves energy and time. However, this technology needs special implements such as seed planter. Given that direct planting is practiced in undisturbed lands, so it was needed to design a special furrow opener. In order to obtain a suitable furrow opener this experiment was conducted in rain-fed Agricultural Research Institute in Maragheh. Materials and Methods Most of seed planters that are used for cultivation in rain fed conditions are equipped by hoe-type furrow opener. Hoe-type furrow openers have good penetration in hard and dry soils. However, they do not have the ability for direct planting. Hoe-type furrow opener was chosen as a model. Then by changing the geometric form of the depth to width ratio (d/w, the two openers were designed. In the first design, which was called O1 two wings and a narrow blade acting as a coulter were added in front of the hoe-type furrow opener. In the second design, which was called O2, in addition to the O1 modification, furrow opener width was decreased and a disk blade was added for seed sowing (Fig. 1. The performance of O1 and O2 openers were compared with the conventional hoe-type furrow opener (check in soil bin and in field conditions. At three different forward speeds (1, 1.5 and 2 m.s-1 with 3 replications, the effects of the openers designs of vertical and horizontal soil forces were evaluated in soil-bin conditions. In order to evaluate the performance of the furrow opener in field conditions, an experiment was conducted using a split plot design based on RCBD at 4 replications. Furrow openers formed the main plots and forward speeds formed the sub plots. Each plot size was 22 meters long in two rows for each treatment. After germination of wheat crop, the numbers of seedlings in two rows were counted (along a one meter. After crop maturity, all plots were harvested by hand and

  14. Preface: SciDAC 2009 (United States)

    Simon, Horst


    and posters goes to the teams of researchers, the success of this year's conference is due to the strong efforts and support from members of the 2009 SciDAC Program Committee and Organizing Committee, and I would like to extend my heartfelt thanks to them for helping to make the 2009 meeting the largest and most successful to date. Program Committee members were: David Bader, LLNL; Pete Beckman, ANL; John Bell, LBNL; John Boisseau, University of Texas; Paul Bonoli, MIT; Hank Childs, LBNL; Bill Collins, LBNL; Jim Davenport, BNL; David Dean, ORNL; Thom Dunning, NCSA; Peg Folta, LLNL; Glenn Hammond, PNNL; Maciej Haranczyk, LBNL; Robert Harrison, ORNL; Paul Hovland, ANL; Paul Kent, ORNL; Aram Kevorkian, SPAWAR; David Keyes, Columbia University; Kwok Ko, SLAC; Felice Lightstone, LLNL; Bob Lucas, ISI/USC; Paul Mackenzie, Fermilab; Tony Mezzacappa, ORNL; John Negele, MIT; Jeff Nichols, ORNL; Mike Norman, UCSD; Joe Oefelein, SNL; Jeanie Osburn, NRL; Peter Ostroumov, ANL; Valerio Pascucci, University of Utah; Ruth Pordes, Fermilab; Rob Ross, ANL; Nagiza Samatova, ORNL; Martin Savage, University of Washington; Tim Scheibe, PNNL; Ed Seidel, NSF; Arie Shoshani, LBNL; Rick Stevens, ANL; Bob Sugar, UCSB; Bill Tang, PPPL; Bob Wilhelmson, NCSA; Kathy Yelick, NERSC/LBNL; Dave Zachmann, Vista Computational Technology LLC. Organizing Committee members were: Communications: Jon Bashor, LBNL. Contracts/Logistics: Mary Spada and Cheryl Zidel, ANL. Posters: David Bailey, LBNL. Proceedings: John Hules, LBNL. Proceedings Database Developer: Beth Cerny Patino, ANL. Program Committee Liaison/Conference Web Site: Yeen Mankin, LBNL. Tutorials: David Skinner, NERSC/LBNL. Visualization Night: Hank Childs, LBNL; Valerio Pascucci, Chems Touati, Nathan Galli, and Erik Jorgensen, University of Utah. Again, my thanks to all. Horst Simon San Diego, California June 18, 2009

  15. Preface: SciDAC 2008 (United States)

    Stevens, Rick


    Microsoft Research at a new conference facility in Redmond, Washington. Over 90 people attended the tutorials, which covered topics ranging from an introduction to BG/P programming to advanced numerical libraries. The SciDAC and INCITE programs and the DOE Office of Advanced Scientific Computing Research core program investments in applied mathematics, computer science, and computational and networking facilities provide a nearly optimum framework for advancing computational science for DOE's Office of Science. At a broader level this framework also is benefiting the entire American scientific enterprise. As we look forward, it is clear that computational approaches will play an increasingly significant role in addressing challenging problems in basic science, energy, and environmental research. It takes many people to organize and support the SciDAC conference, and I would like to thank as many of them as possible. The backbone of the conference is the technical program; and the task of selecting, vetting, and recruiting speakers is the job of the organizing committee. I thank the members of this committee for all the hard work and the many tens of conference calls that enabled a wonderful program to be assembled. This year the following people served on the organizing committee: Jim Ahrens, LANL; David Bader, LLNL; Bryan Barnett, Microsoft; Peter Beckman, ANL; Vincent Chan, GA; Jackie Chen, SNL; Lori Diachin, LLNL; Dan Fay, Microsoft; Ian Foster, ANL; Mark Gordon, Ames; Mohammad Khaleel, PNNL; David Keyes, Columbia University; Bob Lucas, University of Southern California; Tony Mezzacappa, ORNL; Jeff Nichols, ORNL; David Nowak, ANL; Michael Papka, ANL; Thomas Schultess, ORNL; Horst Simon, LBNL; David Skinner, LBNL; Panagiotis Spentzouris, Fermilab; Bob Sugar, UCSB; and Kathy Yelick, LBNL. I owe a special thanks to Mike Papka and Jim Ahrens for handling the electronic theater. I also thank all those who submitted videos. It was a highly successful experiment. Behind the

  16. Without 'Focus'

    Directory of Open Access Journals (Sweden)

    Aldo Sevi


    Full Text Available It is widely accepted that a notion of 'focus', more or less as conceived of in Jackendoff (1972, must be incorporated into our theory of grammar, as a means of accounting for certain observed correlations between prosodic facts and semantic/pragmatic facts. In this paper, we put forth the somewhat radical idea that the time has come to give up this customary view, and eliminate 'focus' from our theory of grammar. We argue that such a move is both economical and fruitful.Research over the years has revealed that the correlations between prosody, 'focus', and the alleged semantic/pragmatic effects of focus are much less clear and systematic than we may have initially hoped. First we argue that this state of affairs detracts significantly from the utility of our notion of 'focus', to the point of calling into question the very motivation for including it in the grammar. Then we look at some of the central data, and show how they might be analyzed without recourse to a notion of 'focus'. We concentrate on (i the effect of pitch accent placement on discourse congruence, and (ii the choice of 'associate' for the so-called 'focus sensitive' adverb only. We argue that our focus-free approach to the data improves empirical coverage, and begins to reveal patterns that have previously been obscured by preconceptions about 'focus'.ReferencesBeaver, D. & Clark, B. 2008. Sense and Sensitivity: How Focus Determines Meaning. Blackwell.Beaver, D., Clark, B., Flemming, E., Jaeger, T. F. & Wolters, M. 2007. ‘When semantics meets phonetics: Acoustical studies of second occurrence focus’. Language 83.2: 245–76., M. & Hirschberg, J. 1994. ‘The ToBI Annotation Conventions’. Ms.,, D. 1972. ‘Accent is predictable (if you are a mind-reader’. Language 48.3: 633–44.üring, D. 2006. ‘Focus projection and default

  17. Browning boreal forests of western North America (United States)

    Verbyla, David


    's boreal forest Remote Sens. 2 2729-47 Riordan B, Verbyla D and McGuire A D 2006 Shrinking ponds in subarctic Alaska based on 1950-2002 remotely sensed images J. Geophys. Res. 111 G04002 Ruess R W, McFarland J M, Trummer L M and Rohrs-Richey J K 2009 Disease-mediated declines in N-fixation inputs by Alnus tenuifolia to early-successional floodplains in interior and south-central Alaska Ecosystems 12 489-502 Stafford J M, Wendler G and Curtis J 2000 Temperature and precipitation of Alaska: 50 year trend analysis Theor. Appl. Climatology 67 33-44 Stow D, Peterson A, Hope A, Engstrom R and Coulter L 2007 Greenness trends of Arctic tundra vegetation in the 1990s: comparison of two NDVI data sets from NOAA AVHRR systems Int. J. Remote Sens. 28 4807-22 van Mantgem P J et al 2009 Widespread increase of tree mortality rates in the western United States Science 323 521-4 Walsh, J E, Chapman W L, Romanovsky V, Christensen J H and Stendel M 2008 Global climate model performance over Alaska and Greenland J. Clim. 21 6156-74 Wendler G and Shulski M 2009 A century of climate change for Fairbanks, Alaska Arctic 62 295-300 Zhang K, Kimball J S, Hogg E H, Zhao M, Oechel W C, Cassano J J and Running S W 2008 Satellite-based model detection of recent climate-driven changes in northern high-latitude vegetation productivity J. Geophys. Res. 113 G03033

  18. only: A Case Study In Projective Meaning

    Directory of Open Access Journals (Sweden)

    Craige Roberts


    Full Text Available I offer an integrated theory of the meaning of only in which the prejacent, while not presupposed, is both entailed and backgrounded, hence tends to project (following a general proposal about projection due to Simons et al. 2010. Moreover, I argue, contra Beaver & Brady (2008, that only is not conventionally associated with focus, the focus effects arising instead pragmatically. But I do adopt aspects of their semantics for only, including the presupposition of a pre-order over the elements of its domain.ReferencesAbbott, B. 2000. ‘Presuppositions as non-assertions’. Journal of Pragmatics 32: 1419–37., B. 2008. ‘Presuppositions and common ground’. Linguistics and Philosophy 31: 523–538., P. 2010. ‘Entailment, assertion, and textual coherence: the case of almost and barely’. Linguistics 48, no. 3: 525–545., P., Roberts, C. & Allyn Smith, E. 2008. ‘Review of The Logic of Conventional Implicature by Chris Potts’. Linguistics and Philosophy 30: 707–749., J. D. 1993. ‘The importance of being “only”: testing the neo-Gricean versus neoentailment paradigms’. Journal of Semantics 10: 301–18., D. 2001. Presupposition and Assertion in Dynamic Semantics. CSLI Publications, Stanford, CA.Beaver, D. 2010. ‘Handout from a talk on projective meaning’. Stuttgart, Germany.Beaver, D. & Brady, C. 2003. ‘Always and Only: Why not all focus sensitive operators are alike’. Natural Language Semantics 11: 323–62., D. & Brady, C. 2008. Sense and Sensitivity. Blackwell., M. E. & Ayers, G. M. 1994. ‘Guidelines for ToBI labeling guide, ver. 2.0’. On-line at The Ohio State University: www