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Sample records for birdsfoot trefoil seed

  1. Study of the Relationships between Technological and Productivity Elements that Determine Seed Yield in Birdsfoot Trefoil (Lotus Corniculatus L.

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    Neculai Dragomir

    2011-10-01

    Full Text Available During an experience performed to elaborate an efficient technology of seed production in birdsfoot trefoil, we observed a series of correlations between yield and the elements of productivity, which exert a strong influence on seed quantity. In this viewpoint, the matrix of correlations between the elements of fructification, in the case of the influence exerted by birdsfoot trefoil cultivation method, made evident the following correlation coefficients: r = 0.81**, between the number of pods/plant; r = 0.82**, between the number of generative sprigs and the number of pods/inflorescence; r = 0.98**, between the number of generative sprigs and the number of pods/plant.

  2. Selection and characterization of glyphosate tolerance in birdsfoot trefoil (Lotus corniculatus)

    International Nuclear Information System (INIS)

    Boerboom, C.M.

    1989-01-01

    If birdsfoot trefoil (Lotus corniculatus L.) was tolerant to glyphosate [N-(phosphonomethyl)glycine], Canada thistle [Cirsium arvense (L.) Scop.] and other dicot weeds could be selectively controlled in certified seed production fields. Glyphosate tolerance in birdsfoot trefoil was identified in plants from the cultivar Leo, plants regenerated from tolerant callus, and selfed progeny of plants regenerated from callus. Plants from the three sources were evaluated in field studies for tolerance to glyphosate at rates up to 1.6 kg ae/ha. Plants of Leo selected for tolerance exhibited a twofold range in the rate required to reduce shoot weight 50% (I 50 s from 0.6 to 1.2 kg/ha glyphosate). Plants regenerated from tolerant callus had tolerance up to 66% greater than plants regenerated from unselected callus. Transgressive segregation for glyphosate tolerance was observed in the selfed progeny of two regenerated plants that both had I 50 s of 0.7 kg/ha glyphosate. The selfed progeny ranged from highly tolerant (I 50 of 1.5 kg/ha) to susceptible (I 50 of 0.5 kg/ha). Spray retention, 14 C-glyphosate absorption and translocation did not account for the differential tolerance of nine plants that were evaluated from the three sources. The specific activity of 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase ranged from 1.3 to 3.5 nmol/min sm-bullet mg among the nine plants and was positively correlated with glyphosate tolerance. Leo birdsfoot trefoil was found to have significant variation in glyphosate tolerance which made it possible to initiate a recurrent selection program to select for glyphosate tolerance in birdsfoot trefoil. Two cycles of selection for glyphosate tolerance were practiced in three birdsfoot trefoil populations, Leo, Norcen, and MU-81

  3. Photosynthate partitioning and nitrogen fixation of alfalfa and birdsfoot trefoil

    International Nuclear Information System (INIS)

    Shieh, W.J.

    1985-01-01

    Nodule mass and number are usually correlated with rates of nitrogen fixation in legumes. Birdsfoot trefoil (Lotus corniculatus L.) with more than twice the nodule number and mass, however, fixes far less nitrogen than alfalfa (Medicago sativa L.) at the same age. In this research, photosynthesis and photosynthate partitioning and utilization in relation to nitrogen fixation of alfalfa and birdsfoot trefoil were examined in order to determine their relationship to nitrogen fixation potential. Photosynthate to nodules was studied using 14 CO 2 labeling techniques. Partitioning patterns were altered by shading and dark depletion treatments. Efficiency of photosynthate utilization was examined by determining turnover of 14 C photosynthate in nodule metabolites and by studying rates of cyanide-resistant and cyanide-sensitive O 2 uptake. Alfalfa nodule activity was greater than trefoil expressed on a hole pot or nodule dry weight basis. Both shading and dark treatments significantly reduced nodule activity as estimated by the acetylene reduction assay. Shoots of both species were found to be the dominant sinks for photosynthate. Percentage 14 C recovered in alfalfa roots was more than twice that of trefoil at 1,2,3,4 and 24 h after labeling. Greater relative specific radioactivity (RSA) in nodules of both species suggests that they were stronger sinks for current photosynthate than roots

  4. Induction of two mutants in birdsfoot trefoil (Lotus corniculatus) by x-rays and chemical mutagens

    Energy Technology Data Exchange (ETDEWEB)

    Therrien, M.C.; Grant, W.F. (McGill Univ., Ste. Anne de Bellevue, Quebec (Canada). Macdonald Coll.)

    1982-10-01

    The mutagenic effects of X-rays, ethyl methanesulfonate (EMS), 8-ethoxycaffeine (EOC), N-hydroxyurea (HU) and 2-aminopurine (2AP) on seed treatment of birdsfoot trefoil (Lotus corniculatus L. 'Mirabel') were assessed over four generations. Mutants were recovered in the M/sub 2/, M/sub 3/ and M/sub 4/ generations from selfed lines, from crosses derived form selfed lines and from open pollination lines. Mutant plants exhibiting vestigial floret character were recovered from X-rays, EMS, EOC and HU treatments. Mutant chlorotica plants were obtained from EMS treatment only. No mutants were recovered from 2AP treatment, EMS, the most effective mutagen, produced nine vestigial floret and 12 chlorotica mutants. Mutants were obtained from only one exposure of X-rays (12 krad). There was evidence for preferential elimination of gametes. The chlorotica and vestigial floret mutants were inherited as tetrasomic recessives. Mutation frequencies of 0.4 - 3.1% in a tetrasomic background are indicative of the effectiveness of EMS in birdsfoot trefoil.

  5. Induction of two mutants in birdsfoot trefoil (Lotus corniculatus) by x-rays and chemical mutagens

    International Nuclear Information System (INIS)

    Therrien, M.C.; Grant, W.F.

    1982-01-01

    The mutagenic effects of X-rays, ethyl methanesulfonate (EMS), 8-ethoxycaffeine (EOC), N-hydroxyurea (HU) and 2-aminopurine (2AP) on seed treatment of birdsfoot trefoil (Lotus corniculatus L. 'Mirabel') were assessed over four generations. Mutants were recovered in the M 2 , M 3 and M 4 generations from selfed lines, from crosses derived form selfed lines and from open pollination lines. Mutant plants exhibiting vestigial floret character were recovered from X-rays, EMS, EOC and HU treatments. Mutant chlorotica plants were obtained from EMS treatment only. No mutants were recovered from 2AP treatment, EMS, the most effective mutagen, produced nine vestigial floret and 12 chlorotica mutants. Mutants were obtained from only one exposure of X-rays (12 krad). There was evidence for preferential elimination of gametes. The chlorotica and vestigial floret mutants were inherited as tetrasomic recessives. Mutation frequencies of 0.4 - 3.1% in a tetrasomic background are indicative of the effectiveness of EMS in birdsfoot trefoil

  6. CO2 fixation in alfalfa and birdsfoot trefoil root nodules and partitioning of 14C to the plant

    International Nuclear Information System (INIS)

    Maxwell, C.A.; Vance, C.P.; Heichel, G.H.; Stade, S.

    1984-01-01

    The objectives of this study were to determine if nonphotosynthetic CO 2 fixation by root nodules contributes carbon for the assimilation of fixed N 2 in alfalfa (Medicago sativa L.) and birdsfoot trefoil (Lotus corniculatus L.) and if assimilation products are partitioned to different plant organs. Effective alfalfa nodules excised from or attached to roots had apparent 14 CO 2 fixation rates of 50 to 80 μg CO 2 kg -1 s -1 (dry weight) at 0.0012 to 0.0038 mole fraction CO 2 . Nodule CO 2 fixation rates increased six- to seven-fold as ambient CO 2 was raised from 0.0038 to 0.0663 mole fraction. Respiration rates of nodules (3 to 4 mg CO 2 kg -1 s -1 ) were 10 to 100-fold higher than 14 CO 2 fixation rates of nodules. Pulse chase experiments with 14 CO 2 combined with nodule and xylem sap analysis demonstrated the initial products of root and nodule CO 2 fixation were organic acids. However, the export of fixed 14 C from effective nodules was primarily in the form of amino acids. In contrast, nodule and/or root fixed 14 C in ineffectively nodulated alfalfa and denodulated effective alfalfa and birdsfoot trefoil was transported primarily as organic acids. Aspartate, asparagine, alanine, glutamate, and glutamine were the most heavily labeled compounds in the amino acid fraction of both effective alfalfa and birdsfoot trefoil nodules exposed to 14 CO 2 . By contrast, asparate, asparagine, and glutamine were the predominantly labeled amino acids in xylem sap collected from nodulated effective roots exposed to 14 CO 2 . The occurrence of nodule CO 2 fixation in alfalfa and birdsfoot trefoil and the export of fixed carbon as asparagine and aspartate to roots and shoots is consistent with a role for CO 2 fixation by nodules in providing carbon skeletons for assimilation and transport of symbiotically fixed N 2

  7. How planting configuration influences plant secondary metabolites and total N in tall fescue (Festuca arundinacea Schreb.), alfalfa (Medicago sativa L.) and birdsfoot trefoil (Lotus corniculatus L.)

    Science.gov (United States)

    Theories suggest that incorporating alfalfa (Medicago sativa L.; Alf) or birdsfoot trefoil (Lotus corniculatus L.; BFT) into endophyte-infected tall fescue (Festuca arundinaceas Schreb.; E+TF) pasturelands may improve livestock production. We investigated how planting configuration might influence p...

  8. Carcass parameters and meat quality in meat-goat kids finished on chicory, birdsfoot trefoil, or red clover pastures.

    Science.gov (United States)

    Turner, K E; Cassida, K A; Zerby, H N; Brown, M A

    2015-07-01

    This study was conducted during the 2009 and 2010 grazing seasons to assess carcass parameters and chevon (goat meat) quality when meat-goat kids (n=72) were finished on pastures of red clover (Trifolium pratense L.; RCL), birdsfoot trefoil (Lotus corniculatus L.; BFT), or chicory (Cichorium intybus L.; CHIC). Final live weight (Pgoats were finished on RCL compared to CHIC with BFT being intermediate. Ribeye area, backfat thickness, body wall thickness, internal fat score, and leg score were not different (P>0.10) among treatments when adjusted for the covariate of carcass weight. Finishing meat-goat kids on RCL, BFT, or CHIC impacted concentrations of fatty acids (FAs) 18:1 trans-10, 18:1 cis-11, 18:2, 18:3 polyunsaturated fatty acids (PUFAs), omega-6, omega-3, and PUFA:saturated fatty acid ratio in longissimus lumborum samples. Finishing meat-goat kids on CHIC, RCL, or BFT pastures produced carcass weights acceptable for most ethnic markets in the USA. Published by Elsevier Ltd.

  9. Hydrocarbon degradation and plant colonization of selected bacterial strains isolated from the rhizsophere and plant interior of Italian ryegrass and Birdsfoot trefoil

    Science.gov (United States)

    Sohail, Y.; Andria, V.; Reichenauer, T. G.; Sessitsch, A.

    2009-04-01

    Hydrocarbon-degrading strains were isolated from the rhizosphere, root and shoot interior of Italian ryegrass (Lolium multiflorum var. Taurus), Birdsfoot trefoil (Lotus corniculatus var. Leo) grown in a soil contaminated with petroleum oil. Strains were tested regarding their phylogeny and their degradation efficiency. The most efficient strains were tested regarding their suitability to be applied for phytoremediation of diesel oils. Sterilized and non-sterilized agricultural soil, with and with out compost, were spiked with diesel and used for planting Italian ryegrass and birdsfoot trefoil. Four selected strains with high degradation activities, derived from the rhizosphere and plant interior, were selected for individual inoculation. Plants were harvested at flowering stage and plant biomass and hydrocarbon degradation was determined. Furthermore, it was investigated to which extent the inoculant strains were able to survive and colonize plants. Microbial community structures were analysed by 16S rRNA and alkB gene analysis. Results showed efficient colonization by the inoculant strains and improved degradation by the application of compost combined with inoculation as well as on microbial community structures will be presented.

  10. In vitro ruminal fermentation characteristics and utilisable CP supply of sainfoin and birdsfoot trefoil silages and their mixtures with other legumes.

    Science.gov (United States)

    Grosse Brinkhaus, A; Wyss, U; Arrigo, Y; Girard, M; Bee, G; Zeitz, J O; Kreuzer, M; Dohme-Meier, F

    2017-04-01

    The extensive protein degradation occurring during ensiling decreases the nutritive value of silages, but this might be counteracted by tannins. Therefore, silages from two legume species containing condensed tannins (CT) - sainfoin (SF) and birdsfoot trefoil (two cultivars: birdsfoot trefoil, cv. Bull (BTB) and birdsfoot trefoil, cv. Polom) - were compared for their in vitro ruminal fermentation characteristics. The effect of combining them with two CT-free legume silages (lucerne (LU) and red clover (RC)) was also determined. The supply of duodenally utilisable CP (uCP) in the forages was emphasised. The legumes were each harvested from three field sites. After 24 h of wilting on the field, the legumes were ensiled in laboratory silos for 86 days. Proximate constituents, silage fermentation characteristics, CT content and CP fractions were determined. Subsequently, silage samples and 1 : 1 mixtures of the CT-containing and CT-free silages were incubated for 24 h in batch cultures using ruminal fluid and buffer (1 : 2, v/v). Each treatment was replicated six times in six runs. The effects on pH, ammonia and volatile fatty acid concentrations, protozoal counts, and total gas and methane production were determined. uCP content was calculated by considering the CP in the silage and the ammonia in the incubation fluid from treatments and blanks. Statistical evaluation compared data from single plants alone and together with that from the mixtures. Among treatments, SF silage contained the least CP and the most CT. The non-protein nitrogen content was lower, favouring neutral detergent soluble and insoluble protein fractions, in the SF and RC silages. Absolute uCP content was lowest in SF and SF mixtures, although the ratio to total CP was the highest. In comparison with LU, the ammonia concentration of the incubation fluid was lower for SF, RC and BTB and for the mixture of SF with LU. The total gas and methane production was similar among the treatments, and the

  11. Plant secondary metabolites in alfalfa, birdsfoot trefoil, reed canarygrass, and tall fescue unaffected by two different nitrogen sources

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    Plant secondary metabolites (PSM) may increase the sustainability of agriculture systems by reducing inputs, as PSM protect plants against herbivores and pathogens, act as pesticides, insecticides, and anthelmintics, while also attracting pollinators and seed dispersers. Therefore, it is important t...

  12. Calcium-Magnesium salt of saponins from bird's foot trefoil seeds (Lotus corniculatus L.

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    Piotr M. Górecki

    2015-01-01

    Full Text Available A calcium-magnesium salt of saponins was isolated from bird's foot trefoil seeds with a yield of 0.04%. The salt contained: 1.37% - Ca and 0.04% - Mg. The saponin salts were deionized on IR-120 Amberlite and free crystalline saponin was obtained. In both cases the presence of one major component and of three trace ones was found, using TLC. In acid hydrolysates of the saponin salts and of the free saponin the following sugars were identified: rhamaose, xylose, glucose, galactose, glucuronic acid. From among aglycones soyasapogenols B, C, traces of E, and of one unidentified in both cases were found. The Ca-Mg salt obtained seems to be a natural form of saponins in bird's foot trefoil seeds.

  13. Caracterização agronômica de genótipos de cornichão (Lotus corniculatus L. Agronomic characterization if birdsfoot trefoil genotypes (Lotus corniculatus L.

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    Maria Tereza Bolzon Soster

    2004-12-01

    Full Text Available Este trabalho foi conduzido com o objetivo de caracterizar agronomicamente genótipos de cornichão (Lotus corniculatus L., incluindo oito populações do cv. São Gabriel, um cultivar rizomatoso (cv. ARS-2620 e um cultivar sem rizomas (cv. Trueno. Plantas individuais foram cultivadas e submetidas a sete cortes em condições de campo, durante um ano, em Passo Fundo, Rio Grande do Sul, Brasil. Os genótipos apresentaram variabilidade quanto ao comprimento de entrenós, estatura, diâmetro, comprimento de legumes, produção de forragem e valor nutritivo. Na média geral, as populações e o cv. Trueno foram cerca de 50% mais produtivos em relação ao cv. ARS-2620. Os genótipos apresentaram entre 80 e 100% de sobrevivência. O conteúdo de proteína bruta reduziu de 21,8% (estádio vegetativo para 11,2% (estádio de frutificação. A média geral de fibra detergente ácido foi de 24,2% e a de fibra detergente neutro variou entre 55,3 e 58,8%.This work was carried out to characterize agronomically birdsfoot trefoil (Lotus corniculatus L. genotypes, including eight populations derived from the cultivar São Gabriel, one rhizomatous cultivar (ARS-260 and one cultivar without rhizomes (Trueno. Individual plants were submitted to seven cuts under field conditions during one year, at Passo Fundo, Rio Grande do Sul, Brazil. The genotypes showed variability relative to internode lenght, height, diameter, legume lenght, forage production and nutritive value. On the average, the populations and the cv. Trueno were about 50% more productive than the cv. ARS-260. The genotypes presented a survival range from 80% to 100%. The crude protein content decreased from 21.8 (vegetative stage to 11.2% (fruiting stage. The overall mean for acid detergent fiber was 24.2% and the neutral detergent fiber ranged from 55.3% to 58.8%.

  14. Importance and condition of forage crops seed production in agriculture of the Republic of Serbia

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    Đokić Dragoslav

    2013-01-01

    Full Text Available For contemporary and economical livestock production, especially cattle and sheep raising, it is necessary to achieve high production of livestock feed while reducing production costs. Improving the production of perennial grasses and legumes creates a good basis for the development of livestock production in different agro-ecological conditions of Serbia. It also establishes a link between farming and animal husbandry, which is of particular importance for the preservation and higher fertility of arable land and the protection of agro-ecosystems. An important factor for the cheaper production of livestock feed is the possibility to provide sufficient quantities of quality seeds at affordable prices. Production of quality seeds of local varieties of perennial legumes is possible to obtain sufficient amounts of good quality forage. Current situation in forage crop seed production of the Republic of Serbia is unsatisfactory because the seed of perennial grasses are mostly imported. Domestic production of alfalfa, red clover and birdsfoot trefoil met domestic needs only in some years. Seed of imported varieties are often not satisfactory because those varieties are not adapted to our local agro-ecological conditions. The present results provide the basis and direction for further researches that may provide solutions to increase seed yields and which will be widely accepted in practice, which will make the production more cost-effective. Institute for forage crops Kruševac is making a significant contribution to the development of technology of seed productions, especially alfalfa, red clover and perennial grasses. Therefore the role of the Institute is very important and necessary link between production, processing and trading seeds of perennial legumes and grasses in Serbia.

  15. Performance and gastroinstestinal nematode control when meat-goat kids grazed chicory, birdsfoot trefoil, or red clover pasutures

    Science.gov (United States)

    In most pasture-based meat-goat production systems, a major management challenge is control of gastrointestinal nematodes (GIN). Use of legumes and forbs that contain plant secondary compounds may reduce fecal egg count (FEC) and/or improve the overall protein nutrition to help animals better toler...

  16. Trefoil factors in human milk

    DEFF Research Database (Denmark)

    Vestergaard, Else Marie; Nexø, Ebba; Wendt, A

    2008-01-01

    We measured concentrations of the gastrointestinal protective peptides Trefoil factors in human milk. By the use of in-house ELISA we detected high amounts of TFF3, less TFF1 and virtually no TFF2 in human breast milk obtained from 46 mothers with infants born extremely preterm (24-27 wk gestation......), preterm (28-37 wk gestation), and full term (38-42 wk gestation). Samples were collected during the first, second, third to fourth weeks and more than 4 wks postpartum. Median (range) TFF1 [TFF3] concentrations in human milk were 320 (30-34000) [1500 (150-27,000)] pmol/L in wk 1, 120 (30-720) [310 (50......-7100)] pmol/L in wk 2, 70 (20-670) [120 (20-650)] pmol/L in wks 3 to 4, and 60 (30-2500) [80 (20-540)] pmol/L in > 4 wks after delivery. The lowest concentrations of TFF1 and TFF3 were found later than 2 wks after birth. In conclusion, TFF was present in term and preterm human milk with rapidly declining...

  17. 49 CFR Appendix B to Part 172 - Trefoil Symbol

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Trefoil Symbol B Appendix B to Part 172... SECURITY PLANS Pt. 172, App. B Appendix B to Part 172—Trefoil Symbol 1. Except as provided in paragraph 2 of this appendix, the trefoil symbol required for RADIOACTIVE labels and placards and required to be...

  18. Ground cover and tree growth on calcareous minesoils: Greater influence of soil surface than nitrogen rate or seed mix

    International Nuclear Information System (INIS)

    Kost, D.A.; Vimmerstedt, J.P.

    1994-01-01

    Growth of ground cover and trees was evaluated for five growing seasons on calcareous coal minesoil surfaces (standard graded topsoil, graded and ripped topsoil, graded gray cast overburden) in southeastern Ohio. Soil surface plots were seeded in September 1987 with either a standard herbaceous seed mix [orchardgrass (Dactylis glomerata L.), timothy (Phleum pratense L.), perennial ryegrass (Lolium perenne L.), Kentucky bluegrass (Poa pratensis L.), Ranger alfalfa (Medicago sativa L.), Mammoth red clover (Trifolium pratense L.), Empire birdsfoot trefoil (Lotus corniculatus L.), and wheat (Triticum aestivum L.)], or a modified mix using no alfalfa and half the rate of orchardgrass. Nitrogen (45, 90, or 135 kg ha/N) was applied as ammonium nitrate in September 1987 and April 1989. White ash (Fraxinus americana L.), silver maple (Acer saccharinum L.), northern red oak (Quercus rubra L.), and eastern white pine (Pinus strobus L.) were planted in spring 1989 into 0.8 m-wide strips sprayed with glyphosate herbicide at 2.24 kg/ha in October 1988. Total cover and total biomass were highest in July 1989, following the last application of nitrogen fertilizer in April 1989. Total cover ranged from 44% to 56%, and total biomass ranged from 102 to 162 g/0.5 m 2 from 1990 to 1993. Total cover and total biomass were lower at the lowest nitrogen rate in 1989 only. Type of herbaceous seed mix did not affect growth of ground cover or trees. Overall tree survival was 82.0% the first year but declined to 40.6% after 5 yr. Survival varied significantly among all tree species (3.5% for pine, 22.2% for oak, 38.5% for maple, 98.1% for ash)

  19. Effect of trefoil factors on the viscoelastic properties of mucus gels

    DEFF Research Database (Denmark)

    Thim, L; Madsen, F; Poulsen, S S

    2002-01-01

    Trefoil peptides (TFFs) are expressed and secreted in a tissue-specific manner in the gastrointestinal tract. Evidence of coexpression of trefoil peptides and mucins has been demonstrated in most mucus-producing cells in the gastrointestinal tract. The expression of trefoil peptides is up-regulat...

  20. Tissue localization of human trefoil factors 1, 2, and 3

    DEFF Research Database (Denmark)

    Madsen, Jens; Nielsen, Ole; Tornøe, Ida

    2007-01-01

    Trefoil factors (TTFs) are small, compact proteins coexpressed with mucins in the gastrointestinal tract. Three trefoil factors are known in mammals: TFF1, TFF2, and TFF3. They are implicated to play diverse roles in maintenance and repair of the gastrointestinal channel. We compared the expression...... pattern of the three trefoil factors analyzing mRNA from a panel of 20 human tissues by conventional reverse transcriptase (RT) PCR and, in addition, by real-time PCR. These findings were supported by immunohistochemical analysis of paraffin-embedded human tissues using rabbit polyclonal antibodies raised...... against these factors. TFF1 showed highest expression in the stomach and colon, whereas TFF2 and TFF3 showed highest expression in stomach and colon, respectively. All three TFFs were found in the ducts of pancreas. Whereas TFF2 was found to be restricted to these two tissues, the structurally more...

  1. SOLID participatory research from Denmark: Use of herbs in pastures for dairy cows: Farmers’ experience, pasture coverage analyses, and literature survey of Danish research results

    DEFF Research Database (Denmark)

    Kudahl, Anne Braad; Karydi, Emmanouela; Vaarst, Mette

    2015-01-01

    chicory and salad burnet. In both fields, birdsfoot trefoil, yellow sweet clover, Sainfoin and starflower never established although the originally seed mixture had a quite high content of their seeds. The plant coverage analyses of the fields which were one to six years old, generally, dandelions...

  2. Immunoassays of human trefoil factors 1 and 2

    DEFF Research Database (Denmark)

    Vestergaard, E M; Brynskov, J; Ejskjaer, K

    2004-01-01

    The trefoil factors (TFF1-3) are cysteine-rich peptides expressed in the gastrointestinal tract where they play a critical role in mucosal protection and repair. The expression is up-regulated at sites of ulceration in various chronic inflammatory diseases. Recently, we presented an ELISA method...

  3. Circulating serum trefoil factors increase dramatically during pregnancy

    DEFF Research Database (Denmark)

    Samson, Mie Hessellund; Vestergaard, E M; Milman, N

    2008-01-01

    Trefoil factors (TFF1-3) are 7-12 kDa peptides secreted by mucosal surfaces, with changing levels of expression reflected in serum concentrations. The genes for the peptides are located on chromosome 21, the chromosome duplicated in trisomy 21. We studied the levels of circulating TFFs in pregnant...

  4. Promoter hypomethylation and upregulation of trefoil factors in prostate cancer

    DEFF Research Database (Denmark)

    Vestergaard, Else Marie; Nexø, Ebba; Tørring, Niels

    2010-01-01

    cell lines with significant TFF expression as compared to benign immortalized prostate cell lines and PC cell lines not expressing trefoil factor. The most striking difference was observed for CpG sites located close to the AUG start codon overlapping several putative binding sites for cellular...

  5. Allelopathic Effect of Dodder on Different Varieties of Lucerne and Bird’s Foot-Trefoil

    Directory of Open Access Journals (Sweden)

    Valcheva Ekaterina

    2018-03-01

    Full Text Available The aim of this study was to evaluate the allelopathic effect of cold aqueous extracts, both fresh and dry biomass of dodder (Cuscuta epithymum L. on lucerne (Medicago sativa L. and bird’s foot-trefoil (Lotus corniculatus L.. Four different varieties of lucerne “Pleven 6”, “Dara”, “Roly”, “Multifoliolate” and bird’s foot-trefoil (“Gran San Gabriele”, “Leo”, “Local population 1”, “Local population 2” were studied in order to find some varieties with allelopathic tolerance. Ex-situ experiment was carried out as follows: 100 seeds of each variety were put in Petri dishes between filter paper, both cold extracts of parasitic weed biomass were pipetted at a ratio of 1:20 as against the seed mass and then were placed in a thermostat-operated device at a temperature of 22 ± 2°С. Distilled water was used as a control. Percentage inhibition, Index of tolerance and Index of plant development were calculated for assessment of the allelopathic effect of dodder on the early seedling growth, biomass synthesis and initial development of experimental varieties. As a whole, dry weed biomass was found as more toxic for the tested plants than the fresh one. Medicago sativa var. multifoliolate and Lotus corniculatus var. Local population 1 and Local population 2 showed a significant tolerance to the allelopathic influence of Cuscuta epithymum in all studied concentrations of aqueous extract of fresh weed biomass (25, 50 and 100 g l−1 and medium tolerance to aqueous extract of dry weed biomass (concentrations of 25 and 50 g l−1.

  6. Trefoil factor family peptides--friends or foes?

    Science.gov (United States)

    Busch, Maike; Dünker, Nicole

    2015-12-01

    Trefoil factor family (TFF) peptides are a group of molecules bearing a characteristic three-loop trefoil domain. They are mainly secreted in mucous epithelia together with mucins but are also synthesized in the nervous system. For many years, TFF peptides were only known for their wound healing and protective function, e.g. in epithelial protection and restitution. However, experimental evidence has emerged supporting a pivotal role of TFF peptides in oncogenic transformation, tumorigenesis and metastasis. Deregulated expression of TFF peptides at the gene and protein level is obviously implicated in numerous cancers, and opposing functions as oncogenes and tumor suppressors have been described. With regard to the regulation of TFF expression, epigenetic mechanisms as well as the involvement of various miRNAs are new, promising aspects in the field of cancer research. This review will summarize current knowledge about the expression and regulation of TFF peptides and the involvement of TFF peptides in tumor biology and cancerogenesis.

  7. Pharmacokinetics of trefoil peptides and their stability in gastrointestinal contents

    DEFF Research Database (Denmark)

    Kjellev, Stine; Vestergaard, Else Marie; Nexø, Ebba

    2007-01-01

    Trefoil factor family (TFF) peptides are considered promising for therapeutic use in gastrointestinal diseases, and there is a need to explore the fate of injected TFF and the stability of the peptides in the gastrointestinal tract. We studied the pharmacokinetics of intravenously (i.v.) administ......Trefoil factor family (TFF) peptides are considered promising for therapeutic use in gastrointestinal diseases, and there is a need to explore the fate of injected TFF and the stability of the peptides in the gastrointestinal tract. We studied the pharmacokinetics of intravenously (i.......v.) administered hTFF2 in mice and rats and of hTFF3 administered i.v., intramuscularly, intraperitoneally, and subcutaneously in mice, and estimated by ELISA the decay of the peptides added to rat and human gastrointestinal contents. We found that i.v. injected hTFF2 and hTFF3 were cleared from the circulation...

  8. Plasma levels of trefoil factors are increased in patients with advanced prostate cancer

    DEFF Research Database (Denmark)

    Vestergaard, E.M.; Borregaard, Michael Krabbe; Poulsen, Steen Seier

    2006-01-01

    Through cDNA array analyses and immunohistochemistry on tissue microarrays, trefoil factor 3 (TFF3) was recently shown to be overexpressed in prostate cancer. The purpose of this study was to test the feasibility of using the levels of trefoil factors as a plasma marker for prostate cancer....

  9. Quantitative measurements of trefoil factor family peptides: possibilities and pitfalls

    DEFF Research Database (Denmark)

    Samson, Mie Hessellund

    2013-01-01

    The trefoil factor family (TFF) peptides TFF1, TFF2, and TFF3 are produced and secreted by mucous membranes throughout the body. Their importance for the protection and repair of epithelial surfaces is well established, and the three peptides are present in various amounts in mucosal secretions...... as well as in the circulation. They have been linked to both inflammatory diseases and to various types of cancer, and serum concentrations of TFF3 show a more than 47-fold increase during pregnancy. Several both commercial and in-house immunoassays exist, but a number of methodological issues remain...

  10. seeds

    African Journals Online (AJOL)

    Owner

    peptidohydrolase (8.0%) from mung bean seedlings. (Baumgartner and Chrispeels, 1977), EP-HG (4.5%) from horse gram seedlings ( Rajeswari, 1997), acidic protease (15%) from germinating winged-bean seeds. (Usha and Singh, 1996) and EP-1 (1.6%) from barley seedlings and GA3-induced cysteine protease (3.38%).

  11. Urine Trefoil Factors as Prognostic Biomarkers in Chronic Kidney Disease.

    Science.gov (United States)

    Yamanari, Toshio; Sugiyama, Hitoshi; Tanaka, Keiko; Morinaga, Hiroshi; Kitagawa, Masashi; Onishi, Akifumi; Ogawa-Akiyama, Ayu; Kano, Yuzuki; Mise, Koki; Ohmoto, Yasukazu; Shikata, Kenichi; Wada, Jun

    2018-01-01

    Trefoil factor family (TFF) peptides are increased in serum and urine in patients with chronic kidney disease (CKD). However, whether the levels of TFF predict the progression of CKD remains to be elucidated. We determined the TFF levels using peptide-specific ELISA in spot urine samples and performed a prospective cohort study. The association between the levels of urine TFFs and other urine biomarkers as well as the renal prognosis was analyzed in 216 CKD patients (mean age: 53.7 years, 47.7% female, 56.9% with chronic glomerulonephritis, and mean eGFR: 58.5 ml/min/1.73 m 2 ). The urine TFF1 and TFF3 levels significantly increased with the progression of CKD stages, but not the urine TFF2 levels. The TFF1 and TFF3 peptide levels predicted the progression of CKD ≥ stage 3b by ROC analysis (AUC 0.750 and 0.879, resp.); however, TFF3 alone predicted CKD progression in a multivariate logistic regression analysis (odds ratio 3.854, 95% confidence interval 1.316-11.55). The Kaplan-Meier survival curves demonstrated that patients with a higher TFF1 and TFF3 alone, or in combination with macroalbuminuria, had a significantly worse renal prognosis. The data suggested that urine TFF peptides are associated with renal progression and the outcomes in patients with CKD.

  12. Nonlinear analysis of sequence symmetry of beta-trefoil family proteins

    Energy Technology Data Exchange (ETDEWEB)

    Li Mingfeng [Biomolecular Physics and Modeling Group, Department of Physics, Huazhong University of Science and Technology, Wuhan 430074, Hubei (China); Huang Yanzhao [Biomolecular Physics and Modeling Group, Department of Physics, Huazhong University of Science and Technology, Wuhan 430074, Hubei (China); Xu Ruizhen [Biomolecular Physics and Modeling Group, Department of Physics, Huazhong University of Science and Technology, Wuhan 430074, Hubei (China); Xiao Yi [Biomolecular Physics and Modeling Group, Department of Physics, Huazhong University of Science and Technology, Wuhan 430074, Hubei (China)]. E-mail: yxiao@mail.hust.edu.cn

    2005-07-01

    The tertiary structures of proteins of beta-trefoil family have three-fold quasi-symmetry while their amino acid sequences appear almost at random. In the present paper we show that these amino acid sequences have hidden symmetries in fact and furthermore the degrees of these hidden symmetries are the same as those of their tertiary structures. We shall present a modified recurrence plot to reveal hidden symmetries in protein sequences. Our results can explain the contradiction in sequence-structure relations of proteins of beta-trefoil family.

  13. Aspectos térmico-biológicos da germinação de sementes de cornichão anual sob diferentes temperaturas Thermal-biological aspects on seed germination of hairy bird's-foot trefoil under different temperatures

    Directory of Open Access Journals (Sweden)

    Rodrigo Ramos Lopes

    2011-10-01

    Full Text Available O objetivo neste trabalho foi determinar as temperaturas mínima, ótima e máxima para a germinação de sementes de Lotus subbiflorus Lag. utilizando-se como parâmetros a porcentagem, a velocidade, a frequência, a entropia informacional de germinação e a entalpia líquida de ativação. O experimento foi realizado em dois lotes com quatro subamostras, cada uma de 100 sementes, e os dados obtidos foram submetidos à análise de variância e ao teste de Tukey. Foram utilizadas oito temperaturas constantes (0, 5, 10, 15, 20, 25, 30 e 35 ºC com a presença de fotoperíodo. Esta espécie germina numa faixa de temperatura com valores mínimos entre 0 e 5ºC, ótimos entre 15 e 20 ºC e máximos entre 30 e 35 ºC. A velocidade de germinação apresentou-se linearmente dependente da temperatura. Os maiores valores de velocidade de germinação, que ocorreram entre 15 ºC e 20 ºC abaixo e acima desta faixa decresceram significativamente. Os menores valores de entropia informacional ocorreram entre 15 e 20 ºC, mostrando maior sincronização do processo germinvatio nessas temperaturas. O ponto mínimo da entalpia foi atingido a 278 K e o máximo, a 303 K e 290,5 K, ponto no qual a curva cruzou o eixo da abscissaThe objective of this research was to determine the minimal, optimal and maximal temperatures for seed germination of Lotus subbiflorus Lag., using germination percentage, speed, frequency, informational entropy and enthalpy of activation of germination. The experiment was carried out in two lots with four replicates of 100 seeds each. The data were submitted to variance analysis and means were compared by Tukey's test. Eight constant temperatures (0, 5, 10, 15, 20, 25, 30 and 35 ºC and light exposition (darkness and 8 h of light plus 16 h of darkness were used. This species of seed germinates in a temperature range of minimal values between 0 and 5 ºC, optimal values between 15 and 20 ºC and maximal values between 30 and 35 º

  14. Excitation energy transfer in natural photosynthetic complexes and chlorophyll trefoils: hole-burning and single complex/trefoil spectroscopic studies

    Energy Technology Data Exchange (ETDEWEB)

    Ryszard Jankowiak, Kansas State University, Department of Chemistry, CBC Bldg., Manhattan KS, 66505; Phone: (785) 532-6785

    2012-09-12

    In this project we studied both natural photosynthetic antenna complexes and various artificial systems (e.g. chlorophyll (Chl) trefoils) using high resolution hole-burning (HB) spectroscopy and excitonic calculations. Results obtained provided more insight into the electronic (excitonic) structure, inhomogeneity, electron-phonon coupling strength, vibrational frequencies, and excitation energy (or electron) transfer (EET) processes in several antennas and reaction centers. For example, our recent work provided important constraints and parameters for more advanced excitonic calculations of CP43, CP47, and PSII core complexes. Improved theoretical description of HB spectra for various model systems offers new insight into the excitonic structure and composition of low-energy absorption traps in very several antenna protein complexes and reaction centers. We anticipate that better understanding of HB spectra obtained for various photosynthetic complexes and their simultaneous fits with other optical spectra (i.e. absorption, emission, and circular dichroism spectra) provides more insight into the underlying electronic structures of these important biological systems. Our recent progress provides a necessary framework for probing the electronic structure of these systems via Hole Burning Spectroscopy. For example, we have shown that the theoretical description of non-resonant holes is more restrictive (in terms of possible site energies) than those of absorption and emission spectra. We have demonstrated that simultaneous description of linear optical spectra along with HB spectra provides more realistic site energies. We have also developed new algorithms to describe both nonresonant and resonant hole-burn spectra using more advanced Redfield theory. Simultaneous description of various optical spectra for complex biological system, e.g. artificial antenna systems, FMO protein complexes, water soluble protein complexes, and various mutants of reaction centers

  15. Aortic valvuloplasty of calcific aortic stenosis with monofoil and trefoil balloon catheters: practical considerations

    NARCIS (Netherlands)

    S. Plante (Sylvain); M.J.B.M. van den Brand (Marcel); L.C.P. van Veen; C. di Mario (Carlo); C.E. Essed; K.J. Beatt (Kevin); P.W.J.C. Serruys (Patrick)

    1990-01-01

    textabstractIn order to evaluate the relation between balloon design (monofoil, trefoil) and valvular configuration, experimental aortic valvuloplasty was performed in four post-mortem hearts with calcific aortic stenosis of various morphology. The degree of obstruction of the aortic orifice was

  16. Trefoil factors are expressed in human and rat endocrine pancreas: differential regulation by growth hormone

    DEFF Research Database (Denmark)

    Jackerott, Malene; Lee, Ying C; Møllgård, Kjeld

    2006-01-01

    Trefoil factors (TFFs) 1, 2, and 3 are expressed in mucosal epithelia. TFFs are particular abundant in the intestine in which they play a crucial role in maintenance and restitution of the epithelium. Because pancreas developmentally arises from the primitive foregut, we explored the expression o...

  17. Trefoil factor peptide 3 is positively correlated with the viscoelastic properties of the cervical mucus plug

    DEFF Research Database (Denmark)

    Bastholm, Sara Kjær; Samson, Mie Hesselund; Becher, Naja

    2017-01-01

    The viscoelastic properties of the cervical mucus plug are considered essential for the occlusion of the cervical canal and thereby for protection against ascending infections during pregnancy. Factors controlling this property are virtually unknown. This study explores a possible role of trefoil...

  18. Trefoil factor family peptides in the human foetus and at birth

    DEFF Research Database (Denmark)

    Samson, Mie Hessellund; Poulsen, Steen Seier; Obeid, Rima

    2011-01-01

    Eur J Clin Invest 2011; 41 (7): 785-792 ABSTRACT: Background Trefoil factors (TFF1-3) are cysteine-rich peptides secreted by mucosal surfaces. Changing levels of expression are reflected in serum concentrations. Serum levels of TFF2 and TFF3 are highly elevated during pregnancy. Here, we explore ...

  19. BEL β-trefoil: a novel lectin with antineoplastic properties in king bolete (Boletus edulis) mushrooms.

    Science.gov (United States)

    Bovi, Michele; Cenci, Lucia; Perduca, Massimiliano; Capaldi, Stefano; Carrizo, Maria E; Civiero, Laura; Chiarelli, Laurent R; Galliano, Monica; Monaco, Hugo L

    2013-05-01

    A novel lectin was purified from the fruiting bodies of king bolete mushrooms (Boletus edulis, also called porcino, cep or penny bun). The lectin was structurally characterized i.e its amino acid sequence and three-dimensional structure were determined. The new protein is a homodimer and each protomer folds as β-trefoil domain and therefore we propose the name Boletus edulis lectin (BEL) β-trefoil to distinguish it from the other lectin that has been described in these mushrooms. The lectin has potent anti-proliferative effects on human cancer cells, which confers to it an interesting therapeutic potential as an antineoplastic agent. Several crystal forms of the apoprotein and of complexes with different carbohydrates were studied by X-ray diffraction. The structure of the apoprotein was solved at 1.12 Å resolution. The interaction of the lectin with lactose, galactose, N-acetylgalactosamine and T-antigen disaccharide, Galβ1-3GalNAc, was examined in detail. All the three potential binding sites present in the β-trefoil fold are occupied in at least one crystal form and are described in detail in this paper. No important conformational changes are observed in the lectin when comparing its co-crystals with carbohydrates with those of the ligand-free protein.

  20. Evolution of trefoil factor(s: genetic and spatio-temporal expression of trefoil factor 2 in the chicken (Gallus gallus domesticus.

    Directory of Open Access Journals (Sweden)

    Zhengyu Jiang

    Full Text Available Trefoil factors are essential healing initiators participating in mucosal reconstitution and tissue morphogenesis, especially on the surfaces of the gastrointestinal tract. This family has been cloned and characterized predominantly from mammals and amphibians. Avian species ingest stone and grit to help digest food, which may expose their gut to severe physical conditions. To further the understanding of the function of the TFF gene family across species, we undertook this research to clone, sequence, and characterize the spatio-temporal expression patterns of chicken TFF2 (ChTFF2 cDNA. Bioinformatics analysis of the promoter region and deduced amino acid sequence demonstrated that ChTFF2 contained unique characteristics; specifically the chicken promoter has multiple start sites and the protein contains a series of Lys-Lys-Val repeats. Unlike mammals, where TFF2 is detected primarily in the stomach, and occasionally in the proximal duodenum, chicken TFF2 transcripts are found throughout the gastrointestinal tract, with major expression sites in the glandular and muscular stomach as well as evident expression in the colon, small intestine, cecal tonsil and crop. Temporal analysis of intestinal ChTFF2 transcripts by quantitative RT-PCR showed high levels in embryos and a trend of constant expression during embryonic and post-hatch development, with a reduction occurring around hatch. Phylogenetic analysis highlighted the conservation of TFF proteins and functional divergence of trefoil domains, which suggest a transitional role in the bird during evolution.

  1. Hydrogen sulfide upregulated mRNA expressions of sodium bicarbonate cotransporter1, trefoil factor1 and trefoil factor2 in gastric mucosa in rats.

    Science.gov (United States)

    Cheraghi, Parisa; Mard, Seyyed Ali; Nagi, Tahereh

    2016-01-01

    Hydrogen sulfide (H 2 S) has been shown to protect the gastric mucosa through several protective mechanisms but till now its effect on mRNA expression of sodium bicarbonate cotransporter 1 (NBC1), trefoil factor1 (TFF1) and trefoil factor2 (TFF2) was not investigated. This study was aimed to evaluate the effect of H 2 S on mRNA expression of NBC1, TFF1 and TFF2 in rat gastric mucosa in response to gastric distention. Thirty two rats were randomly assigned into four equal groups. They were control (C), distention (D), propargylglycine (PAG)-, and NaHS-treated groups. To evaluate the effect of exogenous and endogenous H 2 S on gene expression of NBC1, TFF1 and TFF2, two groups of rats were received H 2 S donor, intra-peritoneal NaHS (80 µg Kg -1 ), and PAG (50 mg kg -1 ), accompanied to stimulate the gastric acid secretion, respectively. Under general anesthesia and laparotomy, a catheter was inserted into the stomach through duodenum for instillation of isotonic saline for gastric distention. Ninety min after beginning the experiment, animals were sacrificed and the gastric mucosa was collected to determine total acid content of gastric effluents and to quantify the mRNA expression of studied genes by quantitative real-time polymerase chain reaction (qRT-PCR). Results showed that A) gastric distention increased the level of mRNA expressions of NBC1, TFF1 and TFF2; B) these levels in NaHS-treated rats were significantly higher than those in Distention group; and C) PAG decreased the expression levels of NBC1 and TFF1. The Findings showed H 2 S upregulated gene expression of NBC1, TFF1 and TFF2 in gastric mucosa.

  2. Trefoil factors in saliva and gingival tissues of patients with chronic periodontitis

    DEFF Research Database (Denmark)

    Chaiyarit, Ponlatham; Chayasadom, Anek; Wara-Aswapati, Nawarat

    2012-01-01

    BACKGROUND: Trefoil factors (TFFs) are secreted molecules that are involved in cytoprotection against tissue damage and the immune response. TFFs have been detected in saliva and oral tissues, but their clinical significance has never been investigated in patients with chronic periodontitis....... The objective of this study is to determine whether TFF expression in saliva and gingival tissues is associated with periodontal pathology. METHODS: Saliva and gingival tissue samples were collected from 25 non-periodontitis individuals and 25 patients with chronic periodontitis (CP). Enzyme...... observed in patients with CP (P = 0.003 and P periodontal pathology and number of Porphyromonas gingivalis...

  3. IDENTIFICATION AND OCCURRENCE OF FUSARIUM SPECIES ON SEEDS OF COMMON WETCH, WHITE LUPINE AND SOME WILD LEGUMES

    Directory of Open Access Journals (Sweden)

    Tihomir Miličević

    2013-06-01

    Full Text Available The presence and occurrence of Fusarium species was examined on the seeds of cultivated legumes – common vetch (Vicia sativa, white lupine (Lupinus albus, and wild legumes: bird’s-foot trefoil (Lotus corniculatus, wild alfalfa (Medicago sativa, black locust (Robinia pseudoacacia, honey locust (Gleditsia triacanthos, sweet clover (Melilotus officinalis, bird vetch (Vicia cracca and meadow vetchling (Lathyrus pratensis. Thirteen Fusarium species were identified - F. verticillioides, F. acuminatum, F. avenaceum, F. tricinctum F. oxysporum, F. scirpi, F. semitectum, F. culmorum, F. proliferatum, F. pseudograminearum, F. sporotrichioides, F. sambucinum and F. heterosporum. Species F. verticillioides and F. proliferatum were determined on seeds of the cultivated legumes (common vetch and white lupine. Other 11 Fusarium species were determined on seeds of wild legumes (bird’s-foot trefoil, wild alfalfa, sweet clover and bird vetch among which the most prevalent were species F. avenaceum and F. acuminatum.

  4. Elimination of 137Cs from trefoil (leaf and stem), ''Mitsuba'', cryptotaenia japonica hassk, boiled in a distilled and salted waters

    International Nuclear Information System (INIS)

    Motegi, Misako; Miyake, Sadaaki; Ohsawa, Takashi; Nakazawa, Kiyoaki; Izumo, Yoshiro

    1999-01-01

    Elimination of 137 Cs from highly accumulated trefoil (leaf and stem) through boiling in distilled and salted water were investigated in relation to study the effect of cooking and processing on biochemical states of radionuclides (RI) contaminating in foods. 137 Cs was hardly eliminated from the trefoil immersed in a distilled water at room temperature (about 15degC) during 10 min. 137 Cs was considerably eliminated from the trefoil when boiled in a distilled water, 0.3-3.0% salt concentration of the water and soy sauce: about 40-60% (after 2 min), 70-85% (5 min) and 80-90% (10 min), respectively. Elimination of 137 Cs in the soy sauce (e.g. 77.0±2.9%, at 1% salt concentration after 10 min) was restrictive comparing to that in the salt water (93.4±2.3%). These results are expected to contribute to evaluate the radiation exposure to man when a boiled trefoil contaminating with 137 Cs was ingested. (author)

  5. Increased expression of protease-activated receptor 4 and Trefoil factor 2 in human colorectal cancer.

    Directory of Open Access Journals (Sweden)

    Guoyu Yu

    Full Text Available Protease-activated receptor 4 (PAR4, a member of G-protein coupled receptors family, was recently reported to exhibit decreased expression in gastric cancer and esophageal squamous cancer, yet increased expression during the progression of prostate cancer. Trefoil factor 2 (TFF2, a small peptide constitutively expressed in the gastric mucosa, plays a protective role in restitution of gastric mucosa. Altered TFF2 expression was also related to the development of gastrointestinal cancer. TFF2 has been verified to promote cell migration via PAR4, but the roles of PAR4 and TFF2 in the progress of colorectal cancer are still unknown. In this study, the expression level of PAR4 and TFF2 in colorectal cancer tissues was measured using real-time PCR (n = 38, western blotting (n=38 and tissue microarrays (n = 66. The mRNA and protein expression levels of PAR4 and TFF2 were remarkably increased in colorectal cancer compared with matched noncancerous tissues, especially in positive lymph node and poorly differentiated cancers. The colorectal carcinoma cell LoVo showed an increased response to TFF2 as assessed by cell invasion upon PAR4 expression. However, after intervention of PAR4 expression, PAR4 positive colorectal carcinoma cell HT-29 was less responsive to TFF2 in cell invasion. Genomic bisulfite sequencing showed the hypomethylation of PAR4 promoter in colorectal cancer tissues and the hypermethylation in the normal mucosa that suggested the low methylation of promoter was correlated to the increased PAR4 expression. Taken together, the results demonstrated that the up-regulated expression of PAR4 and TFF2 frequently occurs in colorectal cancer tissues, and that overexpression of PAR4 may be resulted from promoter hypomethylation. While TFF2 promotes invasion activity of LoVo cells overexpressing PAR4, and this effect was significantly decreased when PAR4 was knockdowned in HT-29 cells. Our findings will be helpful in further investigations into the

  6. Promoter polymorphisms in trefoil factor 2 and trefoil factor 3 genes and susceptibility to gastric cancer and atrophic gastritis among Chinese population.

    Science.gov (United States)

    Xu, Qian; Chen, Mo-Ye; He, Cai-Yun; Sun, Li-Ping; Yuan, Yuan

    2013-10-15

    The polymorphisms in trefoil factor (TFF) gene family that protect gastrointestinal epithelium might influence individual vulnerability to gastric cancer (GC) and atrophic gastritis. We used the Sequenom MassARRAY platform to identify the genotypes of TFF2 rs3814896 and TFF3 rs9981660 polymorphisms in 478 GC patients, 652 atrophic gastritis patients, and 724 controls. For the TFF2 rs3814896 polymorphism, in the subgroup aged ≤ 50 years, we found that AG+GG genotypes were associated with a 0.746-fold decreased risk of atrophic gastritis [p=0.023, 95% confidence interval (CI)=0.580-0.960], a 0.626-fold decreased risk of GC (p=0.005, 95% CI=0.451-0.868), and a 0.663-fold decreased risk of diffuse-type GC (p=0.034, 95% CI=0.452-0.970) compared with the common AA genotype. For the TFF3 rs9981660 polymorphism, in the male subgroup, individuals with variant AG+AA genotype were associated with a 0.761-fold decreased risk of diffuse-type GC compared with the common GG genotype (p=0.043, 95% CI=0.584-0.992). Additionally, we found that in subjects aged ≤ 50 years compared with common AA genotype, TFF2 rs3814896 AG+GG genotypes were associated with increased TFF2 mRNA levels in the total gastric cancer specimens and in the diffuse-type gastric cancer specimens; and in males aged ≤ 50 years compared with common GG genotype, TFF3 rs9981660 AA+AG genotypes were associated with TFF3 mRNA levels in diffuse-type gastric cancer tissues and their corresponding non-cancerous tissues. To our knowledge, this is the first report of an association between the TFF2 rs3814896 AG+GG genotypes and decreased risks of GC, diffuse-type GC, and atrophic gastritis in younger people aged ≤ 50 years, and an association between TFF3 rs9981660 AG+AA genotype and decreased risk of diffuse-type GC in men. Moreover, we found that TFF2 rs3814896 AG+GG genotypes in people aged ≤ 50 years and TFF3 rs9981660 AG+AA genotypes in younger males with diffuse-type GC were associated with higher levels of

  7. A novel non-lens betagamma-crystallin and trefoil factor complex from amphibian skin and its functional implications.

    Directory of Open Access Journals (Sweden)

    Shu-Bai Liu

    2008-03-01

    Full Text Available In vertebrates, non-lens betagamma-crystallins are widely expressed in various tissues, but their functions are unknown. The molecular mechanisms of trefoil factors, initiators of mucosal healing and being greatly involved in tumorigenesis, have remained elusive.A naturally existing 72-kDa complex of non-lens betagamma-crystallin (alpha-subunit and trefoil factor (beta-subunit, named betagamma-CAT, was identified from frog Bombina maxima skin secretions. Its alpha-subunit and beta-subunit (containing three trefoil factor domains, with a non-covalently linked form of alphabeta(2, show significant sequence homology to ep37 proteins, a group of non-lens betagamma-crystallins identified in newt Cynops pyrrhogaster and mammalian trefoil factors, respectively. betagamma-CAT showed potent hemolytic activity on mammalian erythrocytes. The specific antiserum against each subunit was able to neutralize its hemolytic activity, indicating that the two subunits are functionally associated. betagamma-CAT formed membrane pores with a functional diameter about 2.0 nm, leading to K(+ efflux and colloid-osmotic hemolysis. High molecular weight SDS-stable oligomers (>240-kDa were detected by antibodies against the alpha-subunit with Western blotting. Furthermore, betagamma-CAT showed multiple cellular effects on human umbilical vein endothelial cells. Low dosages of betagamma-CAT (25-50 pM were able to stimulate cell migration and wound healing. At high concentrations, it induced cell detachment (EC(50 10 nM and apoptosis. betagamma-CAT was rapidly endocytosed via intracellular vacuole formation. Under confocal microscope, some of the vacuoles were translocated to nucleus and partially fused with nuclear membrane. Bafilomycin A1 (a specific inhibitor of the vacuolar-type ATPase and nocodazole (an agent of microtuble depolymerizing, while inhibited betagamma-CAT induced vacuole formation, significantly inhibited betagamma-CAT induced cell detachment, suggesting

  8. Excitonic Coupling in Linear and Trefoil Trimer Perylenediimide Molecules Probed by Single-Molecule Spectroscopy

    KAUST Repository

    Yoo, Hyejin

    2012-10-25

    Perylenediimide (PDI) molecules are promising building blocks for photophysical studies of electronic interactions within multichromophore arrays. Such PDI arrays are important materials for fabrication of molecular nanodevices such as organic light-emitting diodes, organic semiconductors, and biosensors because of their high photostability, chemical and physical inertness, electron affinity, and high tinctorial strength over the entire visible spectrum. In this work, PDIs have been organized into linear (L3) and trefoil (T3) trimer molecules and investigated by single-molecule fluorescence microscopy to probe the relationship between molecular structures and interchromophoric electronic interactions. We found a broad distribution of coupling strengths in both L3 and T3 and hence strong/weak coupling between PDI units by monitoring spectral peak shifts in single-molecule fluorescence spectra upon sequential photobleaching of each constituent chromophore. In addition, we used a wide-field defocused imaging technique to resolve heterogeneities in molecular structures of L3 and T3 embedded in a PMMA polymer matrix. A systematic comparison between the two sets of experimental results allowed us to infer the correlation between intermolecular interactions and molecular structures. Our results show control of the PDI intermolecular interactions using suitable multichromophoric structures. © 2012 American Chemical Society.

  9. Excitonic Coupling in Linear and Trefoil Trimer Perylenediimide Molecules Probed by Single-Molecule Spectroscopy

    KAUST Repository

    Yoo, Hyejin; Furumaki, Shu; Yang, Jaesung; Lee, Ji-Eun; Chung, Heejae; Oba, Tatsuya; Kobayashi, Hiroyuki; Rybtchinski, Boris; Wilson, Thea M.; Wasielewski, Michael R.; Vacha, Martin; Kim, Dongho

    2012-01-01

    Perylenediimide (PDI) molecules are promising building blocks for photophysical studies of electronic interactions within multichromophore arrays. Such PDI arrays are important materials for fabrication of molecular nanodevices such as organic light-emitting diodes, organic semiconductors, and biosensors because of their high photostability, chemical and physical inertness, electron affinity, and high tinctorial strength over the entire visible spectrum. In this work, PDIs have been organized into linear (L3) and trefoil (T3) trimer molecules and investigated by single-molecule fluorescence microscopy to probe the relationship between molecular structures and interchromophoric electronic interactions. We found a broad distribution of coupling strengths in both L3 and T3 and hence strong/weak coupling between PDI units by monitoring spectral peak shifts in single-molecule fluorescence spectra upon sequential photobleaching of each constituent chromophore. In addition, we used a wide-field defocused imaging technique to resolve heterogeneities in molecular structures of L3 and T3 embedded in a PMMA polymer matrix. A systematic comparison between the two sets of experimental results allowed us to infer the correlation between intermolecular interactions and molecular structures. Our results show control of the PDI intermolecular interactions using suitable multichromophoric structures. © 2012 American Chemical Society.

  10. Accumulation of 137Cs in trefoil (leaf and stem), ''Mitsuba'', Cryptotaenia japonica Hassk, immersed in hydroponic culture medium

    International Nuclear Information System (INIS)

    Motegi, Misako; Miyake, Sadaaki; Ohsawa, Takashi; Nakazawa, Kiyoaki; Izumo, Yoshiro

    1998-01-01

    Accumulation of 137 Cs in trefoil (leaf and stem), ''Mitsuba'', Cryptotaenia japonica Hassk, with or without root was investigated to prepare higher radioactive plant in hydroponic culture medium (140-150 Bq/ml). It was found that 137 Cs concentration in plant tissue was increased with time, as high as 1.6 times of that in the culture medium after 4 days. On the other hand, 137 Cs concentration was affected by carrier element (Cs>6 ppm) and coexistent elements in the medium. Radioactivity of the plant after 4 days was shown to be sufficient for successive experiments. (author)

  11. Accumulation of {sup 137}Cs in trefoil (leaf and stem), ``Mitsuba``, Cryptotaenia japonica Hassk, immersed in hydroponic culture medium

    Energy Technology Data Exchange (ETDEWEB)

    Motegi, Misako; Miyake, Sadaaki; Ohsawa, Takashi; Nakazawa, Kiyoaki [Saitama Institute of Public Health, Urawa (Japan); Izumo, Yoshiro

    1998-11-01

    Accumulation of {sup 137}Cs in trefoil (leaf and stem), ``Mitsuba``, Cryptotaenia japonica Hassk, with or without root was investigated to prepare higher radioactive plant in hydroponic culture medium (140-150 Bq/ml). It was found that {sup 137}Cs concentration in plant tissue was increased with time, as high as 1.6 times of that in the culture medium after 4 days. On the other hand, {sup 137}Cs concentration was affected by carrier element (Cs>6 ppm) and coexistent elements in the medium. Radioactivity of the plant after 4 days was shown to be sufficient for successive experiments. (author)

  12. Trefoil Factor 3 as a Novel Biomarker to Distinguish Between Adenocarcinoma and Squamous Cell Carcinoma

    Science.gov (United States)

    Wang, Xiao-Nan; Wang, Shu-Jing; Pandey, Vijay; Chen, Ping; Li, Qing; Wu, Zheng-Sheng; Wu, Qiang; Lobie, Peter E.

    2015-01-01

    Abstract In carcinoma, such as of the lung, the histological subtype is important to select an appropriate therapeutic strategy for patients. However, carcinomas with poor differentiation cannot always be distinguished on the basis of morphology alone nor on clinical findings. Hence, delineation of poorly differentiated adenocarcinoma and squamous cell carcinoma, the 2 most common epithelial-origin carcinomas, is pivotal for selection of optimum therapy. Herein, we explored the potential utility of trefoil factor 3 (TFF3) as a biomarker for primary lung adenocarcinoma and extrapulmonary adenocarcinomas derived from different organs. We observed that 90.9% of lung adenocarcinomas were TFF3-positive, whereas no expression of TFF3 was observed in squamous cell carcinomas. The subtype of lung carcinoma was confirmed by four established biomarkers, cytokeratin 7 and thyroid transcription factor 1 for adenocarcinoma and P63 and cytokeratin 5/6 for squamous cell carcinoma. Furthermore, expression of TFF3 mRNA was observed by quantitative PCR in all of 11 human lung adenocarcinoma cell lines and highly correlated with markers of the adenocarcinomatous lineage. In contrast, little or no expression of TFF3 was observed in 4 lung squamous cell carcinoma cell lines. By use of forced expression, or siRNA-mediated depletion of TFF3, we determined that TFF3 appeared to maintain rather than promote glandular differentiation of lung carcinoma cells. In addition, TFF3 expression was also determined in adenocarcinomas from colorectum, stomach, cervix, esophagus, and larynx. Among all these extrapulmonary carcinomas, 93.7% of adenocarcinomas exhibited TFF3 positivity, whereas only 2.9% of squamous cell carcinomas were TFF3-positive. Totally, 92.9% of both pulmonary and extrapulmonary adenocarcinomas exhibited TFF3 positivity, whereas only 1.5% of squamous cell carcinomas were TFF3-positive. In conclusion, TFF3 is preferentially expressed in adenocarcinoma and may function as an

  13. Trefoil factor-2 reverses airway remodeling changes in allergic airways disease.

    Science.gov (United States)

    Royce, Simon G; Lim, Clarice; Muljadi, Ruth C; Samuel, Chrishan S; Ververis, Katherine; Karagiannis, Tom C; Giraud, Andrew S; Tang, Mimi L K

    2013-01-01

    Trefoil factor 2 (TFF2) is a small peptide with an important role in mucosal repair. TFF2 is up-regulated in asthma, suggesting a role in asthma pathogenesis. Given its known biological role in promoting epithelial repair, TFF2 might be expected to exert a protective function in limiting the progression of airway remodeling in asthma. The contribution of TFF2 to airway remodeling in asthma was investigated by examining the expression of TFF2 in the airway and lung, and evaluating the effects of recombinant TFF2 treatment on established airway remodeling in a murine model of chronic allergic airways disease (AAD). BALB/c mice were sensitized and challenged with ovalbumin (OVA) or saline for 9 weeks, whereas mice with established OVA-induced AAD were treated with TFF2 or vehicle control (intranasally for 14 d). Effects on airway remodeling, airway inflammation, and airway hyperresponsiveness were then assessed, whereas TFF2 expression was determined by immunohistochemistry. TFF2 expression was significantly increased in the airways of mice with AAD, compared with expression levels in control mice. TFF2 treatment resulted in reduced epithelial thickening, subepithelial collagen deposition, goblet-cell metaplasia, bronchial epithelium apoptosis, and airway hyperresponsiveness (all P < 0.05, versus vehicle control), but TFF2 treatment did not influence airway inflammation. The increased expression of endogenous TFF2 in response to chronic allergic inflammation is insufficient to prevent the progression of airway inflammation and remodeling in a murine model of chronic AAD. However, exogenous TFF2 treatment is effective in reversing aspects of established airway remodeling. TFF2 has potential as a novel treatment for airway remodeling in asthma.

  14. Bm-TFF2, a toad trefoil factor, promotes cell migration, survival and wound healing

    International Nuclear Information System (INIS)

    Zhang, Yong; Yu, Guoyu; Xiang, Yang; Wu, Jianbo; Jiang, Ping; Lee, Wenhui; Zhang, Yun

    2010-01-01

    Research highlights: → Bm-TFF2 binds to epithelial cells and induces cell migration and wound healing. → Bm-TFF2 suppresses cell apoptosis. → Bm-TFF2 has no effect on cell proliferation. -- Abstract: Toad skin is naked and continually confronted by various injurious factors. Constant skin renewal and repairs occur frequently. However, the mechanisms of the renewal and repair have not clearly elucidated. In our previous work, a trefoil factor (TFF), Bm-TFF2, has been purified from the Bombina maxima skin and characterized as a platelet agonist. The mRNA of TFFs in toad skin was up-regulated greatly during the metamorphosis, indicating a pivotal role of TFFs in amphibian skin. Here, we presented the effects of Bm-TFF2 on the cell migration, apoptosis and proliferation. Bm-TFF2 bound to epithelial cells and showed strong cell motility activity. At the concentrations of 1-100 nM, Bm-TFF2-induced migration of human epithelial AGS and HT-29 cells, and rat intestinal epithelial IEC-6 cell lines. The in vitro wound healing assay also verified the activity of Bm-TFF2. Bm-TFF2 could also inhibit cell apoptosis induced by ceramide and sodium butyrate. The cell migration-promoting activity was abolished by MEK1 inhibitors, U0126 and PD98059, suggesting that ERK1/2 activation is crucial for Bm-TFF2 to stimulate cell migration. Taken together, Bm-TFF2 promoted wound healing by stimulating cell migration via MAPK pathway and preventing cell apoptosis. The potent biological activity of Bm-TFF2 makes it a useful molecular tool for further studies of structure-function relationship of the related human TFFs.

  15. Bm-TFF2, a toad trefoil factor, promotes cell migration, survival and wound healing

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Yong [Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223 (China); Graduate School of Chinese Academy of Sciences, Beijing 100049 (China); Yu, Guoyu [Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223 (China); Graduate School of Chinese Academy of Sciences, Beijing 100049 (China); Department of Biochemistry, Kunming Medical College, Kunming, Yunnan 650032 (China); Xiang, Yang [Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223 (China); Graduate School of Chinese Academy of Sciences, Beijing 100049 (China); Wu, Jianbo [Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223 (China); Jiang, Ping [Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223 (China); Graduate School of Chinese Academy of Sciences, Beijing 100049 (China); Lee, Wenhui [Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223 (China); Zhang, Yun, E-mail: zhangy@mail.kiz.ac.cn [Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223 (China)

    2010-07-30

    Research highlights: {yields} Bm-TFF2 binds to epithelial cells and induces cell migration and wound healing. {yields} Bm-TFF2 suppresses cell apoptosis. {yields} Bm-TFF2 has no effect on cell proliferation. -- Abstract: Toad skin is naked and continually confronted by various injurious factors. Constant skin renewal and repairs occur frequently. However, the mechanisms of the renewal and repair have not clearly elucidated. In our previous work, a trefoil factor (TFF), Bm-TFF2, has been purified from the Bombina maxima skin and characterized as a platelet agonist. The mRNA of TFFs in toad skin was up-regulated greatly during the metamorphosis, indicating a pivotal role of TFFs in amphibian skin. Here, we presented the effects of Bm-TFF2 on the cell migration, apoptosis and proliferation. Bm-TFF2 bound to epithelial cells and showed strong cell motility activity. At the concentrations of 1-100 nM, Bm-TFF2-induced migration of human epithelial AGS and HT-29 cells, and rat intestinal epithelial IEC-6 cell lines. The in vitro wound healing assay also verified the activity of Bm-TFF2. Bm-TFF2 could also inhibit cell apoptosis induced by ceramide and sodium butyrate. The cell migration-promoting activity was abolished by MEK1 inhibitors, U0126 and PD98059, suggesting that ERK1/2 activation is crucial for Bm-TFF2 to stimulate cell migration. Taken together, Bm-TFF2 promoted wound healing by stimulating cell migration via MAPK pathway and preventing cell apoptosis. The potent biological activity of Bm-TFF2 makes it a useful molecular tool for further studies of structure-function relationship of the related human TFFs.

  16. THE INFLUENCE OF INTERCROPS AND FARMYARD MANURE FERTILIZATION IN CHANGEABLE WEATHER CONDITIONS ON CONSUMPTION VALUE OF POTATO TUBERS

    Directory of Open Access Journals (Sweden)

    ANNA PŁAZA

    2010-10-01

    Full Text Available The paper presents the results of research carried out over 1999-2002 with the aims to determine the influence of intercrops and farmyard manure fertilization on consumption value of potato tubers in changeable weather conditions. The following combinations of intercrops fertilization were taken into account: the control plot (without intercrop fertilization, farmyard manure, undersown crop (birdsfoot trefoil, birdsfoot trefoil + Italian ryegrass, Italian ryegrass, stubble crop (oleiferous radish, oleiferous radish – mulch. The results pointed that, the conditions of vegetation period, significantly modified the consumption values of potato tubers. The consumption value of potato tubers which were fertilized with intercrops was formed on approximated level, as the potato which was fertilized with farmyard manure. The best consumption features, especially taste, had potatoes which were fertilized with birdsfoot trefoil and with the mixture of birdsfoot trefoil and Italian ryegrass.

  17. Elimination of {sup 137}Cs from trefoil (leaf and stem), ``Mitsuba``, cryptotaenia japonica hassk, boiled in a distilled and salted waters

    Energy Technology Data Exchange (ETDEWEB)

    Motegi, Misako; Miyake, Sadaaki; Ohsawa, Takashi; Nakazawa, Kiyoaki [Saitama Inst. of Public Health (Japan); Izumo, Yoshiro

    1999-07-01

    Elimination of {sup 137}Cs from highly accumulated trefoil (leaf and stem) through boiling in distilled and salted water were investigated in relation to study the effect of cooking and processing on biochemical states of radionuclides (RI) contaminating in foods. {sup 137}Cs was hardly eliminated from the trefoil immersed in a distilled water at room temperature (about 15degC) during 10 min. {sup 137}Cs was considerably eliminated from the trefoil when boiled in a distilled water, 0.3-3.0% salt concentration of the water and soy sauce: about 40-60% (after 2 min), 70-85% (5 min) and 80-90% (10 min), respectively. Elimination of {sup 137}Cs in the soy sauce (e.g. 77.0{+-}2.9%, at 1% salt concentration after 10 min) was restrictive comparing to that in the salt water (93.4{+-}2.3%). These results are expected to contribute to evaluate the radiation exposure to man when a boiled trefoil contaminating with {sup 137}Cs was ingested. (author)

  18. Metabolism of oral trefoil factor 2 (TFF2) and the effect of oral and parenteral TFF2 on gastric and duodenal ulcer healing in the rat

    DEFF Research Database (Denmark)

    Poulsen, Steen Seier; Thulesen, J; Christensen, L

    1999-01-01

    Trefoil factors (TFFs) are peptides produced by mucus-secreting cells in the gastrointestinal tract. A functional association between these peptides and mucus, leading to stabilisation of the viscoelastic gel overlying the epithelia, has been suggested. Both oral and parenteral administration of ...

  19. Trefoil bundles of NPD 7-element size fuel irradiated to 9100 MWd/tonne U

    Energy Technology Data Exchange (ETDEWEB)

    Bain, A S; Christie, J; Daniel, A R

    1964-01-15

    NPD prototype elements (1 in. OD, 19 in. long) were assembled into trefoil bundles and irradiated in the X-5 pressurized-water loop of NRX. The first tests were for only a few weeks but showed that elements made by sheathing UO{sub 2} pellets in Zircaloy-2 behaved well under irradiation; later similar elements were irradiated for 18000 hours to a burn-up of 9100 MWd/tonne U at {integral}kd{theta} = 40 W/cm. The dimensional stability of all the elements was good. Only those subjected to long irradiation showed progressive diametral increases, and these were attributed to relocation of the UO{sub 2} during interim inspections. Length measurements demonstrated that pellet end-dishing is effective in controlling axial expansion, but that for a given depth of dishing the amount of expansion depends on the shoulder width. The extent of grain growth in the UO{sub 2} was compatible with previously reported results when the duration of irradiation, density of the fuel, and variations in growth characteristics of the different batches of UO{sub 2} are considered. The elements taken to high irradiation released up to 135 ml of fission-product gases, which is 2% of the amount formed. The transverse tensile strength of ring samples from the Zircaloy-2 sheaths increased from 75000 to 95000 lb/in{sup 2} at room temperature, but the ductility dropped. The completely brittle fracture of some rings was due to ZrH{sub 2} precipitation. The failure of one element was caused by increased stress due to a higher heat rating, combined with low ductility of the Zircaloy-2 resulting from radiation damage and with precipitation of ZrH{sub 2} because of a lower coolant temperature. The fission-product release from the split was not excessive, and the element was easily withdrawn from the loop after operating at full power for four days from the time of the failure. (author)

  20. (Heckel) seeds

    African Journals Online (AJOL)

    UTILISATEUR

    Garcinia kola seeds to six different hormonal pre-germination treatments. This consisted of ... Thus, seed dormancy in this case is not a coat- imposed .... development of the cultivation of the species. The cause .... Hormonal regulation of seed ...

  1. PPAR-alpha agonist treatment increases trefoil factor family-3 expression and attenuates apoptosis in the liver tissue of bile duct-ligated rats.

    Science.gov (United States)

    Karakan, Tarkan; Kerem, Mustafa; Cindoruk, Mehmet; Engin, Doruk; Alper, Murat; Akın, Okan

    2013-01-01

    Peroxisome proliferators-activated receptor alpha activation modulates cholesterol metabolism and suppresses bile acid synthesis. The trefoil factor family comprises mucin-associated proteins that increase the viscosity of mucins and help protect epithelial linings from insults. We evaluated the effect of short-term administration of fenofibrate, a peroxisome proliferators activated receptor alpha agonist, on trefoil factor family-3 expression, degree of apoptosis, generation of free radicals, and levels of proinflammatory cytokines in the liver tissue of bile duct-ligated rats. Forty male Wistar rats were randomly divided into four groups: 1 = sham operated, 2 = bile duct ligation, 3 = bile duct-ligated + vehicle (gum Arabic), and 4 = bile duct-ligated + fenofibrate (100 mg/kg/day). All rats were sacrificed on the 7 th day after obtaining blood samples and liver tissue. Liver function tests, tumor necrosis factor-alpha and interleukin 1 beta in serum, and trefoil factor family-3 mRNA expression, degree of apoptosis (TUNEL) and tissue malondialdehyde (malondialdehyde, end-product of lipid peroxidation by reactive oxygen species) in liver tissue were evaluated. Fenofibrate administration significantly reduced serum total bilirubin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyl transferase, and tumor necrosis factor-alpha and interleukin-1β levels. Apoptosis and malondialdehyde were significantly reduced in the fenofibrate group. Trefoil factor family-3 expression increased with fenofibrate treatment in bile duct-ligated rats. The peroxisome proliferators-activated receptor alpha agonist fenofibrate significantly increased trefoil factor family-3 expression and decreased apoptosis and lipid peroxidation in the liver and attenuated serum levels of proinflammatory cytokines in bile duct-ligated rats. Further studies are needed to determine the protective role of fenofibrate in human cholestatic disorders.

  2. Investigation of trefoil factor expression in saliva and oral mucosal tissues of patients with oral squamous cell carcinoma

    DEFF Research Database (Denmark)

    Chaiyarit, Ponlatham; Utrawichian, Akasith; Leelayuwat, Chanvit

    2012-01-01

    Objectives The aims of our study were to determine levels of trefoil factor (TFF) peptides in saliva and oral mucosal tissues from patients with oral squamous cell carcinoma (OSCC), and to evaluate whether individual members of TFFs (TFF1, TFF2, and TFF3) might act as biomarkers of disease....... Materials and methods Saliva samples were from 23 healthy subjects and 23 OSCC patients. Tissue samples were collected from 32 normal oral mucosa (NOM) and 32 OSCC biopsy specimens. ELISA and immunohistochemical methods were used to evaluate the expression of TFF1, TFF2, and TFF3 in saliva and oral mucosal...... tissues, respectively. Results Expression of TFF2 and TFF3 in oral mucosal tissues of OSCC patients was strongly downregulated when compared to healthy subjects (p 

  3. Effect of trefoil factor 3 on intestinal mucous barrier in rats with nonalcoholic steatohepatitis

    Directory of Open Access Journals (Sweden)

    LIANG Kai

    2017-08-01

    Full Text Available ObjectiveTo investigate the change in intestinal mucous barrier in rats with nonalcoholic steatohepatitis (NASH, the effect of trefoil factor 3 (TFF3 on intestinal mucous barrier in NASH rats, and the therapeutic effect of TFF3 on NASH. MethodsA total of 60 clean male Sprague-Dawley rats were randomly divided into normal group, model group, and treatment group, with 20 rats in each group. The rats in the normal group were given normal diet, and those in the model group and the treatment group were given high-fat diet to induce NASH. The rats in the treatment group were given intraperitoneal injection of rhTFF3 at a dose of 1 ml/kg/d (a concentration of 0.1 mg/ml, and those in the normal group and the model group were given normal saline at a dose of 1 ml/kg/d; the course of treatment was 3 weeks for all groups. At the end of week 15, fluorescein isothiocyanate-labeled dextran was given by gavage to evaluate intestinal permeability, and after the rats were sacrificed, serum levels of aspartate aminotransferase (AST, alanine aminotransferase (ALT, total cholesterol (TC, triglyceride (TG, and endotoxin (ET and diamine oxidase (DAO activity were measured. HE staining was performed to observe the histopathological changes of the liver and the terminal ileum, PAS staining was performed to observe and count the goblet cells of the terminal ileum, immunohistochemistry was used to measure the expression of the tight junction protein Occludin and TFF3 in the terminal ileum, and quantitative real-time PCR was used to measure the mRNA transcription level of TFF3. A one-way analysis of variance was used for comparison between multiple groups, and the least significant difference t-test was used for further comparison between any two groups. ResultsThe model group had significant increases in serum levels of AST, ALT, TC, TG, and ET and DAO activity, and the treatment group had significant reductions compared with the model group (all P<0.01. The model

  4. Host-derived, pore-forming toxin-like protein and trefoil factor complex protects the host against microbial infection.

    Science.gov (United States)

    Xiang, Yang; Yan, Chao; Guo, Xiaolong; Zhou, Kaifeng; Li, Sheng'an; Gao, Qian; Wang, Xuan; Zhao, Feng; Liu, Jie; Lee, Wen-Hui; Zhang, Yun

    2014-05-06

    Aerolysins are virulence factors belonging to the bacterial β-pore-forming toxin superfamily. Surprisingly, numerous aerolysin-like proteins exist in vertebrates, but their biological functions are unknown. βγ-CAT, a complex of an aerolysin-like protein subunit (two βγ-crystallin domains followed by an aerolysin pore-forming domain) and two trefoil factor subunits, has been identified in frogs (Bombina maxima) skin secretions. Here, we report the rich expression of this protein, in the frog blood and immune-related tissues, and the induction of its presence in peritoneal lavage by bacterial challenge. This phenomena raises the possibility of its involvement in antimicrobial infection. When βγ-CAT was administrated in a peritoneal infection model, it greatly accelerated bacterial clearance and increased the survival rate of both frogs and mice. Meanwhile, accelerated Interleukin-1β release and enhanced local leukocyte recruitments were determined, which may partially explain the robust and effective antimicrobial responses observed. The release of interleukin-1β was potently triggered by βγ-CAT from the frog peritoneal cells and murine macrophages in vitro. βγ-CAT was rapidly endocytosed and translocated to lysosomes, where it formed high molecular mass SDS-stable oligomers (>170 kDa). Lysosomal destabilization and cathepsin B release were detected, which may explain the activation of caspase-1 inflammasome and subsequent interleukin-1β maturation and release. To our knowledge, these results provide the first functional evidence of the ability of a host-derived aerolysin-like protein to counter microbial infection by eliciting rapid and effective host innate immune responses. The findings will also largely help to elucidate the possible involvement and action mechanisms of aerolysin-like proteins and/or trefoil factors widely existing in vertebrates in the host defense against pathogens.

  5. Beneficial Effects of Temperate Forage Legumes that Contain Condensed Tannins

    Directory of Open Access Journals (Sweden)

    Jennifer W. MacAdam

    2015-07-01

    Full Text Available The two temperate forage legumes containing condensed tannins (CT that promote ruminant production are birdsfoot trefoil (Lotus corniculatus L.; BFT and sainfoin (Onobrychis viciifolia Scop.; SF. Both are well-adapted to the cool-temperate climate and alkaline soils of the Mountain West USA. Condensed tannins comprise a diverse family of bioactive chemicals with multiple beneficial functions for ruminants, including suppression of internal parasites and enteric methane. Birdsfoot trefoil contains 10 to 40 g·CT·kg−1 dry matter (DM, while SF contains 30 to 80 g·CT·kg−1 DM. Our studies have focused on these two plant species and have demonstrated consistently elevated rates of gain for beef calves grazing both BFT and SF. Novel results from our BFT research include carcass dressing percentages and consumer sensory evaluations equivalent to feedlot-finished steers and significantly greater than grass-finished steers, but with omega-3 fatty acid concentrations equal to grass-finished beef. We have further demonstrated that ruminants fed BFT or SF will consume more endophyte-infected tall fescue (Schedonorus arundinaceus (Schreb. Dumort. forage or seed than ruminants fed a non-CT forage legume. There is great potential value for sustainable livestock production in the use of highly digestible, nitrogen-fixing legumes containing tannins demonstrated to improve ruminant productivity.

  6. Endometriosis Leads to an Increased Trefoil Factor 3 Concentration in the Peritoneal Cavity but Does Not Alter Systemic Levels.

    Science.gov (United States)

    Henze, Diana; Doecke, Wolf-Dietrich; Hornung, Daniela; Agueusop, Inoncent; von Ahsen, Oliver; Machens, Kathrin; Schmitz, Arndt A; Gashaw, Isabella

    2017-02-01

    This study analyzed whether trefoil factor 3 (TFF3) is locally elevated and correlated with common biomarkers and inflammatory processes in endometriosis. Peritoneal fluid (PF) was obtained from 50 women and serum from 124 women with or without endometriosis. Experimental endometriosis was induced in female C57BL/6 mice by syngeneic transplantation of uterine tissue to the abdominal wall. Levels of TFF3 in PF of women with endometriosis were significantly increased ( P endometriosis: cancer antigen (CA) 125, CA-19-9, interleukin 8, monocyte chemotactic protein 1, and matrix metalloproteinase 7. Serum levels of TFF3 in women were significantly influenced by the menstrual cycle but were independent from disease state. In mice, local TFF3 levels were significantly elevated in early endometriosis (up to 4 weeks after transplantation, P peritoneal cavity in endometriosis and might play a role in disease pathogenesis and its associated inflammatory processes. Furthermore, the results show that TFF3 is regulated through the menstrual cycle. With respect to animal models, syngeneic mouse model does reflect local TFF3 upregulation in the peritoneal cavity affected by endometriosis.

  7. Host-defense and trefoil factor family peptides in skin secretions of the Mawa clawed frog Xenopus boumbaensis (Pipidae).

    Science.gov (United States)

    Conlon, J Michael; Mechkarska, Milena; Kolodziejek, Jolanta; Leprince, Jérôme; Coquet, Laurent; Jouenne, Thierry; Vaudry, Hubert; Nowotny, Norbert; King, Jay D

    2015-10-01

    Peptidomic analysis of norepinephrine-stimulated skin secretions from the octoploid Mawa clawed frog Xenopus boumbaensis Loumont, 1983 led to the identification and characterization of 15 host-defense peptides belonging to the magainin (two peptides), peptide glycine-leucine-amide (PGLa; three peptides), xenopsin precursor fragment (XPF; three peptides), caerulein precursor fragment (CPF; two peptides), and caerulein precursor fragment-related peptide (CPF-RP; five peptides) families. In addition, caerulein and three peptides with structural similarity to the trefoil factor family (TFF) peptides, xP2 and xP4 from Xenopus laevis were also present in the secretions. Consistent with data from comparisons of the nucleotides sequence of mitochondrial and nuclear genes, the primary structures of the peptides suggest a close phylogenetic relationship between X. boumbaensis and the octoploid frogs Xenopus amieti and Xenopus andrei. As the three species occupy disjunct ranges within Cameroon, it is suggested that they diverged from a common ancestor by allopatric speciation. Copyright © 2015 Elsevier Inc. All rights reserved.

  8. Seed quality in informal seed systems

    NARCIS (Netherlands)

    Biemond, P.C.

    2013-01-01

    Keywords: informal seed systems, seed recycling, seed quality, germination, seed pathology, seed health, seed-borne diseases, mycotoxigenic fungi, Fusarium verticillioides, mycotoxins, Vigna unguiculata, Zea mays, Nigeria.

    Seed is a crucial input for agricultural production.

  9. seed oils

    African Journals Online (AJOL)

    Timothy Ademakinwa

    processes, production of biodiesel, as lubricant and in deep-frying purposes. They could ... for its juice, nectars and fruit while its seeds are ... Malaysia. The fine seed powder was stored in a plastic container inside a refrigerator at between 4 o.

  10. seed flour

    African Journals Online (AJOL)

    ONOS

    2010-09-06

    Sep 6, 2010 ... and with a nice taste, used for cooking or as lamp oil. The fatty acid ... Pra seeds were obtained from a local market in Nakhon Si Thammarat. Page 2. Table 1. Proximate composition of pra seed flour. Constituent. Percentage ...

  11. Robotic seeding

    DEFF Research Database (Denmark)

    Pedersen, Søren Marcus; Fountas, Spyros; Sørensen, Claus Aage Grøn

    2017-01-01

    Agricultural robotics has received attention for approximately 20 years, but today there are only a few examples of the application of robots in agricultural practice. The lack of uptake may be (at least partly) because in many cases there is either no compelling economic benefit......, or there is a benefit but it is not recognized. The aim of this chapter is to quantify the economic benefits from the application of agricultural robots under a specific condition where such a benefit is assumed to exist, namely the case of early seeding and re-seeding in sugar beet. With some predefined assumptions...... with regard to speed, capacity and seed mapping, we found that among these two technical systems both early seeding with a small robot and re-seeding using a robot for a smaller part of the field appear to be financially viable solutions in sugar beet production....

  12. Seed regulations and local seed systems

    NARCIS (Netherlands)

    Louwaars, N.

    2000-01-01

    Seed regulations have been introduced in most countries based on the development of formal seed production. Concerns about seed quality and about the varietal identity of the seeds have commonly led to seed laws. However, formal regulations are often inappropriate for informal seed systems, which

  13. A chimeric peptide of intestinal trefoil factor containing cholesteryl ester transfer protein B cell epitope significantly inhibits atherosclerosis in rabbits after oral administration.

    Science.gov (United States)

    Qi, Gaofu; Li, Jingjing; Wang, Shengying; Xin, Shanshan; Du, Peng; Zhang, Qingye; Zhao, Xiuyun

    2011-04-01

    Vaccination against cholesteryl ester transfer protein (CETP) is proven to be effective for inhibiting atherosclerosis in animal models. In this study, the proteases-resistant intestinal trefoil factor (TFF3) was used as a molecular vehicle to construct chimeric TFF3 (cTFF3) containing CETP B cell epitope and tetanus toxin helper T cell epitope. It was found that cTFF3 still preserved a trefoil structure, and can resist proteases digestion in vitro. After oral immunization with cTFF3, the CETP-specific IgA and IgG could be found in intestine lavage fluid and serum, and the anti-CETP antibodies could inhibit partial CETP activity to increase high-density lipoprotein cholesterol, decrease low-density lipoprotein cholesterol, and inhibit atherosclerosis in animals. Therefore, TFF3 is a potential molecular vehicle for developing oral peptide vaccines. Our research highlights a novel strategy for developing oral peptide vaccines in the future. Copyright © 2010 Elsevier Inc. All rights reserved.

  14. A Variant Form of the Human Deleted in Malignant Brain Tumor 1 (DMBT1) Gene Shows Increased Expression in Inflammatory Bowel Diseases and Interacts with Dimeric Trefoil Factor 3 (TFF3)

    DEFF Research Database (Denmark)

    Madsen, Jens; Sorensen, Grith Lykke; Nielsen, Ole Stig

    2013-01-01

    The protein deleted in malignant brain tumors (DMBT1) and the trefoil factor (TFF) proteins have all been proposed to have roles in epithelial cell growth and cell differentiation and shown to be up regulated in inflammatory bowel diseases. A panel of monoclonal antibodies was raised against human...

  15. Site-characteristic expression and induction of trefoil factor family 1, 2 and 3 and malignant brain tumor-1 in normal and diseased intrahepatic bile ducts relates to biliary pathophysiology

    DEFF Research Database (Denmark)

    Sasaki, Motoko; Tsuneyama, Koichi; Saito, Takahito

    2004-01-01

    BACKGROUND/AIM: Trefoil factor family (TFF)1,2,3 are involved in a homeostasis/repair process of mucosal epithelia. In this study, the significance of TFF family and deleted in the malignant brain tumor-1 (DMBT1), a putative receptor of TFF2, in the intrahepatic biliary tree was investigated...

  16. Enhanced antidepressant-like effects of the macromolecule trefoil factor 3 by loading into negatively charged liposomes

    Directory of Open Access Journals (Sweden)

    Qin J

    2014-11-01

    Full Text Available Jing Qin,1 Xu Yang,1–3 Jia Mi,4 Jianxin Wang,1 Jia Hou,1,2 Teng Shen,1 Yongji Li,2 Bin Wang,4 Xuri Li,4 Weili Zhu5 1Department of Pharmaceutics, School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, Shanghai, 2Department of Pharmaceutics, School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, 3Department of Pharmacy, The Fifth People’s Hospital of Shanghai, Fudan University, Shanghai, 4Binzhou Medical University, Yantai, 5National Institute on Drug Dependence, Peking University, Beijing, People’s Republic of China Abstract: Immunocytes, mainly neutrophils and monocytes, exhibit an intrinsic homing property, enabling them to migrate to sites of injury and inflammation. They can thus act as Trojan horses carrying concealed drug cargoes while migrating across impermeable barriers to sites of disease, especially the blood–brain barrier (BBB. In this study, to target circulating phagocytic cells, we formulated negatively charged nanosize liposomes and loaded trefoil factor 3 (TFF3 into liposomes by the pH-gradient method. According to the optimized formulation (5:1.5 of lipid to cholesterol, 10:1 of lipid to drug, 10 mg/mL of lipid concentration, and 10 mmol/L of phosphate-buffered saline, 44.47% entrapment efficiency was obtained for TFF3 liposomes with 129.6 nm particle size and –36.6 mV zeta potential. Compared with neutrally charged liposomes, the negatively charged liposomes showed a strong binding capacity with monocytes and were effectively carried by monocytes to cross the BBB in vitro. Furthermore, enhanced antidepressant-like effects were found in the tail-suspension and forced-swim tests in mice, as measured by decreased immobility time, as well as increased swimming time and reduced immobility in rats. These results suggested that negatively charged liposomes could improve the behavioral responses of TFF3, and our study opens up a new way for the development of

  17. Effects of feeding dairy cows different legume-grass silages on milk phytoestrogen concentration

    DEFF Research Database (Denmark)

    Höjer, A; Adler, S; Purup, Stig

    2012-01-01

    interval of legume-grass silage on phytoestrogen intake and milk phytoestrogen concentrations. In one experiment, 15 Swedish Red dairy cows were fed 2- or 3-cut red clover-grass silage, or 2-cut birdsfoot trefoil-grass silage. In a second experiment, 16 Norwegian Red dairy cows were fed short-term ley...... red clover-grass silage diet (1,494μg/kg of milk). Because of the metabolism of biochanin A, genistein, and prunetin, their concentrations in milk and the apparent recovery were low. Coumestrol was detected in only short-term and long-term ley silage mixtures, and its milk concentration was low....... Concentrations of secoisolariciresinol and matairesinol were higher in 2-cut birdsfoot trefoil-grass and long-term ley silage mixtures, those with legume species other than red clover, and the highest grass proportions. The 2-cut birdsfoot trefoil-grass silage diet also resulted in higher enterolactone...

  18. seed oil

    African Journals Online (AJOL)

    Wara

    Neem seed oil from the neem tree (Azadiracta indica) finds wide usage one of which is its utilization for cosmetics particularly .... obtained which is higher than that of olive oil 17. mgKOH/g (Davine ... The skin tolerance of shea fat employed as ...

  19. Utilização de cama sobreposta de suínos e sobressemeadura de leguminosas para aumento da produção e qualidade de pastagem natural Swine deep bedding amendment and legume sod-seeding to increase production and quality of natural pasture

    Directory of Open Access Journals (Sweden)

    Paulo Hentz

    2008-09-01

    Full Text Available Este trabalho foi conduzido com o objetivo de verificar o efeito da fertilização com dois tipos de camas sobrepostas de suínos (CSS, à base de casca de arroz (CA e maravalha (MA, sobre a produção de uma pastagem natural, com predomínio de Axonopus sp. e Paspalum sp., sobressemeada com leguminosas (cornichão e trevo-branco. As camas sobrepostas foram aplicadas em quantidades equivalentes a 180, 360, 540 e 720 kg/ha de P2O5, constituindo quatro níveis de cama de casca de arroz (30, 60, 90 ou 120 t/ha e quatro níveis de cama de maravalha (20,6; 41,2; 62,2 ou 82,8 t/ha. Foram incluídos dois tratamentos-testemunha: 180 kg/ha de superfosfato triplo (SFT e ausência de adubação (AA. A sobressemeadura das leguminosas foi realizada em maio de 2004 e, a partir de agosto de 2004, foram realizados nove cortes da pastagem, a cada seis semanas. As maiores produções de matéria seca (MS anuais (setembro/2004 a agosto/2005 com as aplicações de cama de casca de arroz foram obtidas nos níveis 30 e 60 t/ha e, com cama de maravalha, nos níveis 62,2 e 82,8 t/ha, que resultaram em produções de MS de 8.083 e 8.276 kg/ha, superiores às obtidas com superfosfato triplo (4.091 kg MS/ha e sem adubação (3.071 MS kg/ha. A produção de MS de trevo-branco aumentou linearmente no outono e no inverno, de acordo com os níveis de adubação com cama de maravalha, mas não variaram com a adubação com cama de casca de arroz. O efeito fertilizante varia entre os tipos de cama sobreposta, que podem influenciar a composição botânica de pastagens mistas de leguminosas-gramíneas.This work aimed to evaluate the effect of two swine deep beddings (SDB: rice husk (CA and sawdust coarse (MA on the production of natural pasture. The predominant species in this pasture were Axonopus sp. and Paspalum sp. that was sod-seeded with the forage legumes birdsfoot trefoil and white clover. The SDBs were applied in amounts equivalent to 180, 360, 540, and 720 kg/ha of P2O

  20. α-Tocopherol and β-carotene contents of forage species in four-cut system

    DEFF Research Database (Denmark)

    Lindquist, H.; Nadeau, A.; Jensen, Søren Krogh

    2014-01-01

    α-Tocopherol and β-carotene concentrations were measured in herbage of birdsfoot trefoil (Bft), red clover (Rc), white clover (Wc), timothy (Ti) and perennial ryegrass (Pr) grown in four mixtures (Bft+Ti, Rc+Ti, Rc+Pr and Wc+Pr) in a small-plot field experiment at Foulum, Denmark, over 2 years. P......-carotene concentrations were 54 and 62 mg kg−1 DM. In conclusion, herbage of birdsfoot trefoil generally had higher vitamin concentrations than white or red clover, but differences between the two grass species were small and inconsistent....

  1. Organic leek seed production - securing seed quality

    DEFF Research Database (Denmark)

    Deleuran, Lise Christina; Boelt, Birte

    2011-01-01

    To maintain integrity in organic farming, availability of organically produced GM-free seed of varieties adapted to organic production systems is of vital impor-tance. Despite recent achievements, organic seed supply for a number of vegetable species is insufficient. Still, in many countries...... seeds. Tunnel production is a means of securing seed of high genetic purity and quality, and organic leek (Allium porrum L.) seed production was tested in tunnels in Denmark. The present trial focused on steckling size and in all years large stecklings had a positive effect on both seed yield...

  2. Organic Leek Seed Production - Securing Seed Quality

    DEFF Research Database (Denmark)

    Deleuran, L C; Boelt, B

    2011-01-01

    To maintain integrity in organic farming, availability of organically produced GM-free seed of varieties adapted to organic production systems is of vital impor-tance. Despite recent achievements, organic seed supply for a number of vegetable species is insufficient. Still, in many countries...... seeds. Tunnel production is a means of securing seed of high genetic purity and quality, and organic leek (Allium porrum L.) seed production was tested in tunnels in Denmark. The present trial focused on steckling size and in all years large stecklings had a positive effect on both seed yield...

  3. Localization of trefoil factor family peptide 3 (TFF3) in epithelial tissues originating from the three germ layers of developing mouse embryo.

    Science.gov (United States)

    Bijelić, Nikola; Belovari, Tatjana; Tolušić Levak, Maja; Baus Lončar, Mirela

    2017-08-20

    Trefoil factor family (TFF) peptides are involved in the maintenance of epithelial integrity and epithelial restitution. Mature epithelial tissues originate from different embryonic germ layers. The objective of this research was to explore the presence and localization of TFF3 peptide in mouse embryonic epithelia and to examine if the occurrence of TFF3 peptide is germ layer-dependent. Mouse embryos (14-18 days old) were fixed in 4% paraformaldehyde and embedded in paraffin. Immunohistochemistry was performed with affinity purified rabbit anti-TFF3 antibody, goat anti-rabbit biotinylated secondary antibody and streptavidin-horseradish peroxidase, followed by 3,3'-diaminobenzidine. TFF3 peptide was present in the gastric and intestinal mucosa, respiratory mucosa in the upper and lower airways, pancreas, kidney tubules, epidermis, and oral cavity. The presence and localization of TFF3 peptide was associated with the embryonic stage and tissue differentiation. TFF3 peptide distribution specific to the germ layers was not observed. The role of TFF3 peptide in cell migration and differentiation, immune response, and apoptosis might be associated with specific embryonic epithelial cells. TFF3 peptide may also be considered as a marker for mucosal maturation.

  4. Localization of trefoil factor family peptide 3 (TFF3 in epithelial tissues originating from the three germ layers of developing mouse embryo

    Directory of Open Access Journals (Sweden)

    Nikola Bijelić

    2017-08-01

    Full Text Available Trefoil factor family (TFF peptides are involved in the maintenance of epithelial integrity and epithelial restitution. Mature epithelial tissues originate from different embryonic germ layers. The objective of this research was to explore the presence and localization of TFF3 peptide in mouse embryonic epithelia and to examine if the occurrence of TFF3 peptide is germ layer-dependent. Mouse embryos (14-18 days old were fixed in 4% paraformaldehyde and embedded in paraffin. Immunohistochemistry was performed with affinity purified rabbit anti-TFF3 antibody, goat anti-rabbit biotinylated secondary antibody and streptavidin-horseradish peroxidase, followed by 3,3'-diaminobenzidine. TFF3 peptide was present in the gastric and intestinal mucosa, respiratory mucosa in the upper and lower airways, pancreas, kidney tubules, epidermis, and oral cavity. The presence and localization of TFF3 peptide was associated with the embryonic stage and tissue differentiation. TFF3 peptide distribution specific to the germ layers was not observed. The role of TFF3 peptide in cell migration and differentiation, immune response, and apoptosis might be associated with specific embryonic epithelial cells. TFF3 peptide may also be considered as a marker for mucosal maturation.

  5. Trefoil factor 3 is required for differentiation of thyroid follicular cells and acts as a context-dependent tumor suppressor.

    Science.gov (United States)

    Abols, A; Ducena, K; Andrejeva, D; Sadovska, L; Zandberga, E; Vilmanis, J; Narbuts, Z; Tars, J; Eglitis, J; Pirags, V; Line, A

    2015-01-01

    Trefoil factor 3 (TFF3) is overexpressed in a variety of solid epithelial cancers, where it has been shown to promote migration, invasion, proliferation, survival and angiogenesis. On the contrary, in the majority of thyroid tumors, it is downregulated, yet its role in the development of thyroid cancer remains unknown. Here we show that TFF3 exhibits strong cytoplasmic staining of normal thyroid follicular cells and colloid and the staining is increased in hyperfunctioning thyroid nodules, while it is decreased in all thyroid cancers of follicular cell origin. By meta-analysis of gene expression datasets, we found that in the thyroid cancer, conversely to the breast cancer, the expression of TFF3 mRNA was downregulated by estrogen signaling and confirmed this by treating thyroid cancer cells with estradiol. Forced expression of TFF3 in anaplastic thyroid cancer cells resulted in decreased cell proliferation, clonal spheroid formation and entry into the S phase. Furthermore, it induced acquisition of epithelial-like cell morphology and expression of the differentiation markers of thyroid follicular cells and transcription factors implicated in the thyroid morphogenesis and function. Taken together, this study provides the first evidence that TFF3 may act as a tumor suppressor or an oncogene depending on the cellular context.

  6. Doğal florada yetişen sarıçiçekli gazal boynuzu (Lotus corniculatus L. ve dar yapraklı gazal boynuzunun (Lotus tenuis Waldst. & Kit. toprak tercihleri, komşu bitkileri ve yem değerleri

    Directory of Open Access Journals (Sweden)

    Ferat Uzun

    2018-02-01

    Full Text Available In this study, soil preferences of wild birdsfoot trefoil (Lotus corniculatus L. and narrowleaf birdsfoot trefoil (L tenuis Waldst. & Kit. species growing in natural flora of the Black Sea Region (Turkey and the plant species which they interact with, as well as their feed values were investigated. Dominant forage species that interact with L. corniculatus and L. tenuis were determined by the visual estimation method at 126 and 86 locations, respectively, and also seed and soil samples from each location were collected. L. corniculatus preferred soils having higher lime (90.9 vs. 66.4 g kg-1, P=0.003, pH (7.41 vs. 7.14, P=0.001 and containing lower organic matter (20.0 vs. 26.8 g kg-1, P=0.001 compared to L. tenuis. L. corniculatus was neighbor to 89 different species (20.2% legume, 22.5% grass and 57.3% others, whereas L. tenuis was neighbor to 61 different species (41.0% legume, 19.7% grass and 39.3% others. The difference between two species in terms of the frequencies of neighbor plant families was significant (2=10.814, P=0.004. Dominant plant species growing in interaction with these Lotus species were Medicago lupulina, Trifolium pratense, Trifolium repens, Cynodon dactylon, Lolium perenne and Plantago lanceolata. Dactylis glomerata was also neighbor with high frequency to L. corniculatus. L. tenuis had high phosphorus, metabolizable energy and relative feed value, and lower acid and neutral detergent fiber contents. As a result, in the artificial pasture establishments or the improvement of natural rangelands, the aforementioned species growing in harmony in natural environment and exhibiting positive interaction with Lotus species studied should be preferred.

  7. Alpha-tocopherol and β-carotene in legume-grass mixtures as influenced by wilting, ensiling and type of silage additive

    DEFF Research Database (Denmark)

    Lindqvist, H; Nadeau, E; Jensen, Søren Krogh

    2012-01-01

    Effects of wilting, ensiling and type of additive on α-tocopherol and β-carotene contents in legume–grass mixtures were examined. Swards of birdsfoot trefoil + timothy (Bft + Ti), red clover + timothy (Rc + Ti) and red clover + meadow fescue (Rc + Mf) were harvested as a first regrowth in August...

  8. Crystal structure of type I ryanodine receptor amino-terminal [beta]-trefoil domain reveals a disease-associated mutation 'hot spot' loop

    Energy Technology Data Exchange (ETDEWEB)

    Amador, Fernando J.; Liu, Shuang; Ishiyama, Noboru; Plevin, Michael J.; Wilson, Aaron; MacLennan, David H.; Ikura, Mitsuhiko; (Toronto)

    2009-12-01

    Muscle contraction and relaxation is regulated by transient elevations of myoplasmic Ca{sup 2+}. Ca{sup 2+} is released from stores in the lumen of the sarco(endo)plasmic reticulum (SER) to initiate formation of the Ca{sup 2+} transient by activation of a class of Ca{sup 2+} release channels referred to as ryanodine receptors (RyRs) and is pumped back into the SER lumen by Ca{sup 2+}-ATPases (SERCAs) to terminate the Ca{sup 2+} transient. Mutations in the type 1 ryanodine receptor gene, RYR1, are associated with 2 skeletal muscle disorders, malignant hyperthermia (MH), and central core disease (CCD). The evaluation of proposed mechanisms by which RyR1 mutations cause MH and CCD is hindered by the lack of high-resolution structural information. Here, we report the crystal structure of the N-terminal 210 residues of RyR1 (RyR{sub NTD}) at 2.5 {angstrom}. The RyR{sub NTD} structure is similar to that of the suppressor domain of type 1 inositol 1,4,5-trisphosphate receptor (IP3Rsup), but lacks most of the long helix-turn-helix segment of the 'arm' domain in IP3Rsup. The N-terminal {beta}-trefoil fold, found in both RyR and IP{sub 3}R, is likely to play a critical role in regulatory mechanisms in this channel family. A disease-associated mutation 'hot spot' loop was identified between strands 8 and 9 in a highly basic region of RyR1. Biophysical studies showed that 3 MH-associated mutations (C36R, R164C, and R178C) do not adversely affect the global stability or fold of RyRNTD, supporting previously described mechanisms whereby mutations perturb protein-protein interactions.

  9. Giardia co-infection promotes the secretion of antimicrobial peptides beta-defensin 2 and trefoil factor 3 and attenuates attaching and effacing bacteria-induced intestinal disease.

    Science.gov (United States)

    Manko, Anna; Motta, Jean-Paul; Cotton, James A; Feener, Troy; Oyeyemi, Ayodele; Vallance, Bruce A; Wallace, John L; Buret, Andre G

    2017-01-01

    Our understanding of polymicrobial gastrointestinal infections and their effects on host biology remains incompletely understood. Giardia duodenalis is an ubiquitous intestinal protozoan parasite infecting animals and humans. Concomitant infections with Giardia and other gastrointestinal pathogens commonly occur. In countries with poor sanitation, Giardia infection has been associated with decreased incidence of diarrheal disease and fever, and reduced serum inflammatory markers release, via mechanisms that remain obscure. This study analyzed Giardia spp. co-infections with attaching and effacing (A/E) pathogens, and assessed whether and how the presence of Giardia modulates host responses to A/E enteropathogens, and alters intestinal disease outcome. In mice infected with the A/E pathogen Citrobacter rodentium, co-infection with Giardia muris significantly attenuated weight loss, macro- and microscopic signs of colitis, bacterial colonization and translocation, while concurrently enhancing the production and secretion of antimicrobial peptides (AMPs) mouse β-defensin 3 and trefoil factor 3 (TFF3). Co-infection of human intestinal epithelial cells (Caco-2) monolayers with G. duodenalis trophozoites and enteropathogenic Escherichia coli (EPEC) enhanced the production of the AMPs human β-defensin 2 (HBD-2) and TFF3; this effect was inhibited with treatment of G. duodenalis with cysteine protease inhibitors. Collectively, these results suggest that Giardia infections are capable of reducing enteropathogen-induced colitis while increasing production of host AMPs. Additional studies also demonstrated that Giardia was able to directly inhibit the growth of pathogenic bacteria. These results reveal novel mechanisms whereby Giardia may protect against gastrointestinal disease induced by a co-infecting A/E enteropathogen. Our findings shed new light on how microbial-microbial interactions in the gut may protect a host during concomitant infections.

  10. What Are Chia Seeds?

    Science.gov (United States)

    ... your diet? Chia seeds come from the desert plant Salvia hispanica , a member of the mint family. ... ancient Aztec diet. The seeds of a related plant, Salvia columbariae (golden chia), were used primarily by ...

  11. Seeds and Synergies

    International Development Research Centre (IDRC) Digital Library (Canada)

    'Seeds and Synergies presents inspiring evidence of change in practice and policy ... Seeds of inspiration: breathing new life into the formal agricultural research .... and Urban Development and Poverty Alleviation and Agricultural Commodity ...

  12. Seeds as biosocial commons

    NARCIS (Netherlands)

    Patnaik, Archana

    2016-01-01

    This research investigates and describes the conservation and use of Plant Genetic Resources (PGRs), especially seeds through processes of commonisation. Seeds form an important element for sustaining human life (through food production) and social relations (by maintaining agricultural

  13. Seed dispersal in fens

    NARCIS (Netherlands)

    Middleton, Beth; van Diggelen, Rudy; Jensen, Kai

    Question: How does seed dispersal reduce fen isolation and contribute to biodiversity? Location: European and North American fens. Methods: This paper reviews the literature on seed dispersal to fens. Results: Landscape fragmentation may reduce dispersal opportunities thereby isolating fens and

  14. Seed development and carbohydrates

    NARCIS (Netherlands)

    Wittich, P.E.

    1998-01-01

    Seeds assure the plant the onset of a next generation and a way of dispersal. They consist of endosperm and an embryo (originating from gametophytic tissue), enveloped by a seed coat (sporophytic tissue). Plants generate different types of seeds. For instance, the endosperm may either be

  15. Hot seeding using large Y-123 seeds

    International Nuclear Information System (INIS)

    Scruggs, S J; Putman, P T; Zhou, Y X; Fang, H; Salama, K

    2006-01-01

    There are several motivations for increasing the diameter of melt textured single domain discs. The maximum magnetic field produced by a trapped field magnet is proportional to the radius of the sample. Furthermore, the availability of trapped field magnets with large diameter could enable their use in applications that have traditionally been considered to require wound electromagnets, such as beam bending magnets for particle accelerators and electric propulsion. We have investigated the possibility of using large area epitaxial growth instead of the conventional point nucleation growth mechanism. This process involves the use of large Y123 seeds for the purpose of increasing the maximum achievable Y123 single domain size. The hot seeding technique using large Y-123 seeds was employed to seed Y-123 samples. Trapped field measurements indicate that single domain samples were indeed grown by this technique. Microstructural evaluation indicates that growth can be characterized by a rapid nucleation followed by the usual peritectic grain growth which occurs when large seeds are used. Critical temperature measurements show that no local T c suppression occurs in the vicinity of the seed. This work supports the suggestion of using an iterative method for increasing the size of Y-123 single domains that can be grown

  16. Oil palm seed distribution

    Directory of Open Access Journals (Sweden)

    Durand-Gasselin Tristan

    2005-03-01

    Full Text Available For a tropical plant, the oil palm commodity chain has the peculiarity of possessing a major seed production sector for reasons that are primarily genetic. This seed sector has numerous original aspects. Breeders are also propagators and usually also distribute their seeds. Oil palm seeds are semi-recalcitrant: they display pseudo-dormancy. Achieving seed germination is difficult and requires lengthy treatments and special installations. This restriction greatly influences seed distribution and the role of the different stakeholders in the commodity chain. It was only once it had been discovered how the “sh” gene functioned, which controls shell thickness, and when it became necessary to produce “tenera” seeds derived from exclusively “dura x pisifera” crosses, that a true seed market developed. In addition it is difficult to organize seed distribution to smallholders. This is partly due to difficulties that the profession, or a State-run organization, has in controlling middlemen networks, and partly to the absence of any protective systems (UPOV, plant breeder certificate, etc. that generally oblige breeders to preserve and propagate parents in their own installations. In fact there are major inequalities in the access to seeds between agroindustry and smallholders. Another peculiarity of the oil palm seed market is the virtually total absence of guarantees for buyers: the quality of the research conducted by breeders, the seed production strategies necessary for transferring genetic progress, and the technical quality of production. The only guarantee today comes from the relations of confidence established year after year between breeders/distributors and growers. In this fields, research can lead to some proposals: molecular biology offers some interesting prospects for certifying seed quality and social science develop effective communication methods.

  17. Does the informal seed system threaten cowpea seed health?

    NARCIS (Netherlands)

    Biemond, P.C.; Oguntade, O.; Lava Kumar, P.; Stomph, T.J.; Termorshuizen, A.J.; Struik, P.C.

    2013-01-01

    Most smallholder farmers in developing countries depend on an informal Seed System (SS) for their seed. The informal SS is often criticized because farmer-produced seed samples are not tested for seed health, thus accepting the risk of planting infected seeds. Here we aimed at assessing the quality

  18. combining high seed number and weight to improve seed yield

    African Journals Online (AJOL)

    ACSS

    ABSTRACT. Increasing seed size and seed weight is an important trait for trade, yield component and adaptation of chickpea ... determining yield or quality, and the development of rapid and ..... C.G. 1981. Control of seed growth in soybeans.

  19. Producing the target seed: Seed collection, treatment, and storage

    Science.gov (United States)

    Robert P. Karrfalt

    2011-01-01

    The role of high quality seeds in producing target seedlings is reviewed. Basic seed handling and upgrading techniques are summarized. Current advances in seed science and technology as well as those on the horizon are discussed.

  20. Efficiency of alfalfa seed processing with different seed purity

    OpenAIRE

    Đokić, Dragoslav; Stanisavljević, Rade; Terzić, Dragan; Milenković, Jasmina; Radivojević, Gordana; Koprivica, Ranko; Štrbanović, Ratibor

    2015-01-01

    The work was carried out analysis of the impact of the initial purity of raw alfalfa seed on the resulting amount of processed seed in the processing. Alfalfa is very important perennial forage legume which is used for fodder and seed production. Alfalfa seed is possible to achieve high yields and very good financial effects. To obtain the seed material with good characteristics complex machines for cleaning and sorting seeds are used. In the processing center of the Institute for forage crop...

  1. Paper (essay on seed

    Directory of Open Access Journals (Sweden)

    Mirić Mladen

    2013-01-01

    Full Text Available Based on detailed studies of the past of the agrarian thought of the world (evolution of agro-innovation, and within it, the relationship of man and seed, the author has selected key data for Table 1. In addition, more or less known folk sayings, proverbs, curses and allegories in which the seed is the key word have been collected. Then, religious books, folk art and literature works (sayings of prominent individuals and experts and observations of the author himself have been studied. According to the collected opus, it can be concluded that the vast importance of seed meaningfully entwined into all spheres of folk life and cultural heritage. Seed is directly tied to the following eight key (revolutionary milestones in the food and economic life of mankind: 1. the first and for the longest time, the seed used to be the main food of the people and the first food reserve; 2. Neolithic Revolution was simply caused by the sowing of seeds; 3. for the sake of sowing man began to develop more complex processing techniques; 4. everywhere and forever, especially since the late 15th century, the seed has been a carrier of (rescuing plants between Europe and the New World, that is, between continents; 5. seed was the first product that has been chemically treated since the mid-18th century; 6. standard operation procedures and quality are promoted on seed by which it became the first product to have prescribed (compulsory methods, but it also became a good whose quality has to be tested before sale; 7. hybrid seed is a 'perpetrator' of the green revolution in the mid-20th century and at last there is disputable seed of genetically modified organisms that are spreading with certain reactions. The author proposes that the United Nations Standard International Trade Classification includes a special section (division which would classify the seed for sowing, while beyond this Classification terms such as seed, plant seed should be replaced with non-seed

  2. Seed dormancy and germination.

    Science.gov (United States)

    Penfield, Steven

    2017-09-11

    Reproduction is a critical time in plant life history. Therefore, genes affecting seed dormancy and germination are among those under strongest selection in natural plant populations. Germination terminates seed dispersal and thus influences the location and timing of plant growth. After seed shedding, germination can be prevented by a property known as seed dormancy. In practise, seeds are rarely either dormant or non-dormant, but seeds whose dormancy-inducing pathways are activated to higher levels will germinate in an ever-narrower range of environments. Thus, measurements of dormancy must always be accompanied by analysis of environmental contexts in which phenotypes or behaviours are described. At its simplest, dormancy can be imposed by the formation of a simple physical barrier around the seed through which gas exchange and the passage of water are prevented. Seeds featuring this so-called 'physical dormancy' often require either scarification or passage through an animal gut (replete with its associated digestive enzymes) to disrupt the barrier and permit germination. In other types of seeds with 'morphological dormancy' the embryo remains under-developed at maturity and a dormant phase exists as the embryo continues its growth post-shedding, eventually breaking through the surrounding tissues. By far, the majority of seeds exhibit 'physiological dormancy' - a quiescence program initiated by either the embryo or the surrounding endosperm tissues. Physiological dormancy uses germination-inhibiting hormones to prevent germination in the absence of the specific environmental triggers that promote germination. During and after germination, early seedling growth is supported by catabolism of stored reserves of protein, oil or starch accumulated during seed maturation. These reserves support cell expansion, chloroplast development and root growth until photoauxotrophic growth can be resumed. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

  3. A variant form of the human deleted in malignant brain tumor 1 (DMBT1 gene shows increased expression in inflammatory bowel diseases and interacts with dimeric trefoil factor 3 (TFF3.

    Directory of Open Access Journals (Sweden)

    Jens Madsen

    Full Text Available The protein deleted in malignant brain tumors (DMBT1 and the trefoil factor (TFF proteins have all been proposed to have roles in epithelial cell growth and cell differentiation and shown to be up regulated in inflammatory bowel diseases. A panel of monoclonal antibodies was raised against human DMBT1(gp340. Analysis of lung washings and colon tissue extracts by Western blotting in the unreduced state, two antibodies (Hyb213-1 and Hyb213-6 reacted with a double band of 290 kDa in lung lavage. Hyb213-6, in addition, reacted against a double band of 270 kDa in colon extract while Hyb213-1 showed no reaction. Hyb213-6 showed strong cytoplasmic staining in epithelial cells of both the small and large intestine whereas no staining was seen with Hyb213-1. The number of DMBT1(gp340 positive epithelial cells, stained with Hyb213-6, was significantly up regulated in inflammatory colon tissue sections from patients with ulcerative colitis (p<0.0001 and Crohn's disease (p = 0.006 compared to normal colon tissue. Immunohistochemical analysis of trefoil factor TFF1, 2 and 3 showed that TFF1 and 3 localized to goblet cells in both normal colon tissue and in tissue from patients with ulcerative colitis or Crohn's disease. No staining for TFF2 was seen in goblet cells in normal colon tissue whereas the majority of tissue sections in ulcerative colitis and Crohn's disease showed sparse and scattered TFF2 positive goblet cells. DMBT1 and TFF proteins did therefore not co-localize in the same cells but localized in adjacent cells in the colon. The interaction between DMBT1(gp340 and trefoil TFFs proteins was investigated using an ELISA assay. DMBT1(gp340 bound to solid-phase bound recombinant dimeric TFF3 in a calcium dependent manner (p<0.0001 but did not bind to recombinant forms of monomeric TFF3, TFF2 or glycosylated TFF2. This implies a role for DMBT1 and TFF3 together in inflammatory bowel disease.

  4. Artificial Seeds and their Applications

    Indian Academy of Sciences (India)

    currently working on ... heterozygosity of seed, minute seed size, presence of reduced ... Advantages of Artificial or Synthetic Seeds over Somatic Embryos for Propagation .... hour gives optimum bead hardness and rigidity for the produc-.

  5. Seed thioredoxin h

    DEFF Research Database (Denmark)

    Hägglund, Per; Finnie, Christine; Yano, Hiroyuki

    2016-01-01

    , for example chloroplastic f- and m-type thioredoxins involved in regulation of the Calvin-Benson cycle. The cytosolic h-type thioredoxins act as key regulators of seed germination and are recycled by NADPH-dependent thioredoxin reductase. The present review on thioredoxin h systems in plant seeds focuses...

  6. Saving Seed Microbiomes

    NARCIS (Netherlands)

    Berg, Gabriele; Raaijmakers, J.M.

    2018-01-01

    Plant seeds are home to diverse microbial communities whose composition is determined by plant genotype, environment, and management practices. Plant domestication is now recognized as an important driver of plant-associated microbial diversity. To what extent and how domestication affects seed

  7. Glioblastoma with spinal seeding

    International Nuclear Information System (INIS)

    Fakhrai, N.; Fazeny-Doerner, B.; Marosi, C.; Czech, T.; Diekmann, K.; Birner, P.; Hainfellner, J.A.; Prayer, D.

    2004-01-01

    Background: extracranial seeding of glioblastoma multiforme (GBM) is very rare and its development depends on several factors. This case report describes two patients suffering from GBM with spinal seeding. In both cases, the anatomic localization of the primary tumor close to the cerebrospinal fluid (CSF) was the main factor for spinal seeding. Case reports: two patients with GBM and spinal seeding are presented. After diagnosis of spinal seeding, both patients were highly symptomatic from their spinal lesions. Case 1 experienced severe pain requiring opiates, and case 2 had paresis of lower limbs as well as urinary retention/incontinence. Both patients were treated with spinal radiation therapy. Nevertheless, they died 3 months after diagnosis of spinal seeding. Results: in both patients the diagnosis of spinal seeding was made at the time of cranial recurrence. Both tumors showed close contact to the CSF initially. Even though the patients underwent intensive treatment, it was not possible to keep them in a symptom-free state. Conclusion: because of short survival periods, patients deserve optimal pain management and dedicated palliative care. (orig.)

  8. Glioblastoma with spinal seeding

    Energy Technology Data Exchange (ETDEWEB)

    Fakhrai, N.; Fazeny-Doerner, B.; Marosi, C. [Clinical Div. of Oncology, Dept. of Medicine I, Univ. of Vienna (Austria); Czech, T. [Dept. of Neurosurgery, Univ. of Vienna (Austria); Diekmann, K. [Dept. of Radiooncology, Univ. of Vienna (Austria); Birner, P.; Hainfellner, J.A. [Clinical Inst. for Neurology, Univ. of Vienna (Austria); Prayer, D. [Dept. of Neuroradiology, Univ. of Vienna (Austria)

    2004-07-01

    Background: extracranial seeding of glioblastoma multiforme (GBM) is very rare and its development depends on several factors. This case report describes two patients suffering from GBM with spinal seeding. In both cases, the anatomic localization of the primary tumor close to the cerebrospinal fluid (CSF) was the main factor for spinal seeding. Case reports: two patients with GBM and spinal seeding are presented. After diagnosis of spinal seeding, both patients were highly symptomatic from their spinal lesions. Case 1 experienced severe pain requiring opiates, and case 2 had paresis of lower limbs as well as urinary retention/incontinence. Both patients were treated with spinal radiation therapy. Nevertheless, they died 3 months after diagnosis of spinal seeding. Results: in both patients the diagnosis of spinal seeding was made at the time of cranial recurrence. Both tumors showed close contact to the CSF initially. Even though the patients underwent intensive treatment, it was not possible to keep them in a symptom-free state. Conclusion: because of short survival periods, patients deserve optimal pain management and dedicated palliative care. (orig.)

  9. Physalis peruviana seed storage

    Directory of Open Access Journals (Sweden)

    Cíntia L. M. de Souza

    2016-03-01

    Full Text Available ABSTRACT Physalis peruviana belongs to Solanaceae family and has a high nutritional and nutraceutical potential. The production is intended for fruit consumption and the propagation is mainly by seeds. This study aimed to evaluate the influence of priming on the kinetics of germination of P. peruviana seeds stored at different temperatures. The seeds were stored at 5 and 25 °C in a chamber saturated with zinc chloride solution and in liquid nitrogen (-196 °C. Every 4 months, the seeds were removed from storage for evaluation of germination and moisture content in the laboratory and emergence and development of seedlings in greenhouse. During the last evaluation at 16 months, the seeds under the same conditions were subjected to salt stress. The moisture content varied during the storage period, but was always higher for seeds kept at -196 ºC. These seeds kept high germination percentage in water until 16 months, regardless of the tested temperature; however, in salt solution the germination percentage was significantly reduced.

  10. Effects of seed fermentation method on seed germination and vigor ...

    African Journals Online (AJOL)

    The present study was conducted to examine the influence of Lagenaria siceraria seed fermentation method on seed germination and vigor. Three seed fermentation methods (fermented in ambient air, plastic bag stored in ambient or in plastic bag buried) were tested on two cultivars during two years. Seed germination and ...

  11. Prosopis Africana SEEDS (OKPEYE)

    African Journals Online (AJOL)

    User

    Keywords: Prosopis africana, okpeye seeds, thermal heat conductivity, specific heat capacity, thermal heat diffusivity, .... 2.3 Determination of Thermal Properties of Prosopis. Africana .... and the guard ring was filled with fiber glass at both the.

  12. Oil seed marketing prospects

    International Nuclear Information System (INIS)

    Ceroni, G.

    1992-01-01

    With its 100 million tonnes annual production, the American continent is by far the world's biggest producer of oil seed, followed by Asia - 52 million, and Europe - 27 million tonnes. The Italian and European Communities have the farming capacity to double their production, but international agreements currently prohibit such initiatives. After first providing a panorama of the world oil seed market, this paper discusses new reforms in European Communities internal agricultural policies which currently limit production. These reforms, intended to encourage the production of oil seed for use as an ecological automotive fuel alternative, call for an obligatory set-aside of 15% of producing farm-land in exchange for the compensatory removal of oil seed production limits

  13. Genetics and Forest Seed Handling

    DEFF Research Database (Denmark)

    Schmidt, Lars Holger

    2016-01-01

    High genetic quality seed is obtained from seed sources that match the planting site, have a good outcrossing rate, and are superior in some desirable characters. Non-degraded natural forests and plantations may be used as untested seed sources, which can sometimes be managed to promote outbreeding...... and increase seed production. Planted seed orchards aim at capturing large genetic variation and are planted in a design that facilitates genetic evaluation and promotes outbred seed production. Good seed production relies upon success of the whole range of reproductive events from flower differentiation...

  14. Seeds of impurity

    Directory of Open Access Journals (Sweden)

    Andrea Pavoni

    2015-06-01

    Full Text Available Ai Weiwei’s art installation Kui Hua Zi [Sunflower Seeds] took place between 2010 and 2011 in the gigantic Turbine Hall of the Tate Modern Gallery, in London. It consisted of 100 millions hand-crafted porcelain seeds made in Jingdezhen, China.1 An uneven surface to dive into, a haptic space of undulating vision, rustling steps, unusual horizontality, a meaningless quicksand where the separation between artwork and spectator is engulfed, the immunity of distant contemplation denied.

  15. Storage of sunflower seeds

    Directory of Open Access Journals (Sweden)

    Denise de Castro Lima

    Full Text Available The sunflower is among the top five crops in the world for the production of edible vegetable oil. The species displays rustic behavior, with an excellent edaphic and climatic adaptability index, being able to be cultivated throughout Brazil. Seed quality is the key to increasing production and productivity in the sunflower. The objective of this work was to monitor the viability of sunflower seeds with a view to their conservation when stored in different environments and packaging. The seeds were packed in paper bags, multilayered paper, black polyethylene and PET bottles; and stored for a period of twelve months in the following environments: dry cold room (10 ºC and 55% RH, the ambient conditions of Fortaleza, Ceará, Brazil (30-32 ºC and 75% RH, refrigerator (4 ºC and 38-43% RH and freezer (-20 ºC. Every three months, the water content of the seeds was determined and germination, accelerated ageing, speed of emergence index, and seedling dry weight were evaluated. The experimental design was completely randomized, in a scheme of split-lots, with four replications. It can be concluded that the natural environment is not suitable for the storage of sunflower seeds. Sunflower seeds remain viable for 12 months when stored in a dry cold room, refrigerator or freezer, irrespective of the type of packaging used.

  16. Seed dispersal in fens

    Science.gov (United States)

    Middleton, B.; Van Diggelen, R.; Jensen, K.

    2006-01-01

    Question: How does seed dispersal reduce fen isolation and contribute to biodiversity? Location: European and North American fens. Methods: This paper reviews the literature on seed dispersal to fens. Results: Landscape fragmentation may reduce dispersal opportunities thereby isolating fens and reducing genetic exchange. Species in fragmented wetlands may have lower reproductive success, which can lead to biodiversity loss. While fens may have always been relatively isolated from each other, they have become increasingly fragmented in modern times within agricultural and urban landscapes in both Europe and North America. Dispersal by water, animals and wind has been hampered by changes related to development in landscapes surrounding fens. Because the seeds of certain species are long-lived in the seed bank, frequent episodes of dispersal are not always necessary to maintain the biodiversity of fens. However, of particular concern to restoration is that some dominant species, such as the tussock sedge Carex stricta, may not disperse readily between fens. Conclusions: Knowledge of seed dispersal can be used to maintain and restore the biodiversity of fens in fragmented landscapes. Given that development has fragmented landscapes and that this situation is not likely to change, the dispersal of seeds might be enhanced by moving hay or cattle from fens to damaged sites, or by reestablishing lost hydrological connections. ?? IAVS; Opulus Press.

  17. Seed-borne pathogens and electrical conductivity of soybean seeds

    Directory of Open Access Journals (Sweden)

    Adriana Luiza Wain-Tassi

    2012-02-01

    Full Text Available Adequate procedures to evaluate seed vigor are important. Regarding the electrical conductivity test (EC, the interference in the test results caused by seed-borne pathogens has not been clarified. This research was carried out to study the influence of Phomopsis sojae (Leh. and Colletotrichum dematium (Pers. ex Fr. Grove var. truncata (Schw. Arx. fungi on EC results. Soybean seeds (Glycine max L. were inoculated with those fungi using potato, agar and dextrose (PDA medium with manitol (-1.0 MPa and incubated for 20 h at 25 °C. The colony diameter, index of mycelial growth, seed water content, occurrence of seed-borne pathogens, physiological potential of the seeds, measured by germination and vigor tests (seed germination index, cold test, accelerated aging and electrical conductivity, and seedling field emergence were determined. The contents of K+, Ca2+, and Mg2+ in the seed and in the soaking solution were also determined. A complete 2 × 4 factorial design with two seed sizes (5.5 and 6.5 mm and four treatments (control, seeds incubated without fungi, seeds incubated with Phomopsis and seeds incubated with Colletotrichum were used with eight (5.5 mm large seeds and six (6.5 mm large seeds replications. All seeds submitted to PDA medium had their germination reduced in comparison to the control seeds. This reduction was also observed when seed vigor and leached ions were considered. The presence of Phomopsis sojae fungus in soybean seed samples submitted to the EC test may be the cause of misleading results.

  18. The importance of using certified seed

    OpenAIRE

    Bogdanović Sandra; Mladenov Velimir; Balešević-Tubić Svetlana

    2015-01-01

    Certified seed is produced from the seed of known genetic origin and genetic purity with controlled and tested production, processed and declared in accordance with the Law on Seeds. Production of certified seed is carried out under the supervision of the Ministry of Agriculture and Environmental Protection, by seed producers formally listed in the Seed Register. Seed is processed in registered seed processing centres and quality is tested in laboratories accredited for seed testing. The orga...

  19. Magnetic-seeding filtration

    International Nuclear Information System (INIS)

    Ying, T.Y.; Chin, C.J.; Lu, S.C.; Yiacoumi, S.

    1997-10-01

    Magnetic-seeding filtration consists of two steps: heterogeneous particle flocculation of magnetic and nonmagnetic particles in a stirred tank and high-gradient magnetic filtration (HGMF). The effects of various parameters affecting magnetic-seeding filtration (HGMF). The effects of various parameters affecting magnetic seeding filtration are theoretically and experimentally investigated. A trajectory model that includes hydrodynamic resistance, van der Waals, and electrostatic forces is developed to calculate the flocculation frequency in a turbulent-shear regime. Fractal dimension is introduced to simulate the open structure of aggregates. A magnetic-filtration model that consists of trajectory analysis, a particle build-up model, a breakthrough model, and a bivariate population-balance model is developed to predict the breakthrough curve of magnetic-seeding filtration. A good agreement between modeling results and experimental data is obtained. The results show that the model developed in this study can be used to predict the performance of magnetic-seeding filtration without using empirical coefficients or fitting parameters. 35 refs., 7 figs., 1 tab

  20. Molecular physiology of seeds

    International Nuclear Information System (INIS)

    Hajduch, M.

    2014-05-01

    Plant development is well described. However, full understanding of the regulation of processes associated with plant development is still missing. Present Dr.Sc. thesis advances our understanding of the regulation of plant development by quantitative proteomics analyses of seed development of soybean, canola, castor, flax, and model plant arabidopsis in control and environmentally challenged environments. The analysis of greenhouse-grown soybean, canola, castor, and arabidospis provided complex characterization of metabolic processes during seed development, for instance, of carbon assimilation into fatty acids. Furthermore, the analyses of soybean and flax grown in Chernobyl area provided in-depth characterization of seed development in radio-contaminated environment. Soybean and flax were altered by radio-contaminated environment in different way. However, these alterations resulted into modifications in seed oil content. Further analyses showed that soybean and flax possess alterations of carbon metabolism in cytoplasm and plastids along with increased activity of photosynthetic apparatus. Our present experiments are focused on further characterization of molecular bases that might be responsible for alterations of seed oil content in Chernobyl grown plants. (author)

  1. 7 CFR 201.33 - Seed in bulk or large quantities; seed for cleaning or processing.

    Science.gov (United States)

    2010-01-01

    ... quantities; seed for cleaning or processing. (a) In the case of seed in bulk, the information required under... seeds. (b) Seed consigned to a seed cleaning or processing establishment, for cleaning or processing for... pertaining to such seed show that it is “Seed for processing,” or, if the seed is in containers and in...

  2. Improving Soil Seed Bank Management.

    Science.gov (United States)

    Haring, Steven C; Flessner, Michael L

    2018-05-08

    Problems associated with simplified weed management motivate efforts for diversification. Integrated weed management uses fundamentals of weed biology and applied ecology to provide a framework for diversified weed management programs; the soil seed bank comprises a necessary part of this framework. By targeting seeds, growers can inhibit the propagule pressure on which annual weeds depend for agricultural invasion. Some current management practices affect weed seed banks, such as crop rotation and tillage, but these tools are often used without specific intention to manage weed seeds. Difficulties quantifying the weed seed bank, understanding seed bank phenology, and linking seed banks to emerged weed communities challenge existing soil seed bank management practices. Improved seed bank quantification methods could include DNA profiling of the soil seed bank, mark and recapture, or 3D LIDAR mapping. Successful and sustainable soil seed bank management must constrain functionally diverse and changing weed communities. Harvest weed seed controls represent a step forward, but over-reliance on this singular technique could make it short-lived. Researchers must explore tools inspired by other pest management disciplines, such as gene drives or habitat modification for predatory organisms. Future weed seed bank management will combine multiple complementary practices that enhance diverse agroecosystems. This article is protected by copyright. All rights reserved.

  3. Seeds of confusion : the impact of policies on seed systems

    OpenAIRE

    Louwaars, N.P.

    2007-01-01

    Seed is basic to crop production. Next to its importance in production, food security and rural development, seed is a key element in many debates about technology development and transfer, biodiversity, globalisation and equity. The sustainable availability of good quality seed is thus an important development issue. This study deals with the impact different types of regulation have on how farmers access seed. I have analysed current regulatory frameworks in terms of their impact on differe...

  4. Magnetic-seeding filtration

    International Nuclear Information System (INIS)

    Depaoli, D.

    1996-01-01

    This task will investigate the capabilities of magnetic-seeding filtration for the enhanced removal of magnetic and nonmagnetic particulates from liquids. This technology appies to a wide range of liquid wastes, including groundwater, process waters, and tank supernatant. Magnetic-seeding filtration can be used in several aspects of treatment, such as (1) removal of solids, particularly those in the colloidal-size range that are difficult to remove by conventional means; (2) removal of contaminants by precipitation processes; and (3) removal of contaminants by sorption processes

  5. genetics and inheritance of seed dormancy inflicted by seed

    African Journals Online (AJOL)

    Mgina

    ABSTRACT. The study was undertaken to investigate the genetic mode of inheritance of dormancy imposed by the hull (seed coat) in rice seeds. Freshly harvested seeds of parents, F1 and F2 populations of a cross between a dormant cultivar Kisegese and non-dormant strain K2004 were used. Germination test of the ...

  6. Seeds of confusion : the impact of policies on seed systems

    NARCIS (Netherlands)

    Louwaars, N.P.

    2007-01-01

    Seed is basic to crop production. Next to its importance in production, food security and rural development, seed is a key element in many debates about technology development and transfer, biodiversity, globalisation and equity. The sustainable availability of good quality seed is thus an important

  7. Effects of seed collecting date and storage duration on seed ...

    African Journals Online (AJOL)

    The objective of this study was to determine the effect of seed collecting dates (5 to 6 times from mid-November to early January, 10 days intervals) and seed storage duration (4, 8, and 12 months) at room temperature on seed germination of four Artemisia species (Artemisia sieberi, A. diffusa, A. kupetdaghensis, and A.

  8. Cone and seed yields in white spruce seed production areas

    Science.gov (United States)

    John A. Pitcher

    1966-01-01

    The source of seed is an important consideration in the reforestation program on the National Forests in the North Central Region. Thirty-five seed production areas have been set up in the Region, along the lines proposed by the North Central Forest Experiment Station, to provide control of seed source. Red pine, white pine, shortleaf and loblolly pine, and white...

  9. seed germination and seedlings growth

    African Journals Online (AJOL)

    STORAGESEVER

    2007-12-17

    Dec 17, 2007 ... The role of 20E in plant physiology including seed germination is not studied. ..... GA3, ABA and CKs on lettuce Lactuca sativa seed germination are ..... Practical uses for ecdysteroids in mammals and humans: an update. J.

  10. Seed research for improved technologies

    OpenAIRE

    Bino,R.J.; Jalink,H.; Oluoch,M.O.; Groot,S.P.C.

    1998-01-01

    The production of high-quality seed is the basis for a durable a profitable agriculture. After production, seed is processed, conditioned, stored, shipped and germinated. For quality assurance, seed quality has to be controlled at all steps of the production chain. Seed functioning is accompanied by programmed transitions from cell proliferation to quiescence upon maturation and from quiescence to reinitiation of cellular metabolism upon imbibition. Despite the obvious importance of these con...

  11. (Lupinus albus) SEEDS

    African Journals Online (AJOL)

    user

    2010-08-08

    Aug 8, 2010 ... lupin samples indicated that lupins can be used as a raw material for various food ... lupin seeds can be utilized for milk and meat imitation products. ... estimated by multiplying the percentage of crude protein, crude fat and ...

  12. Seed for change

    NARCIS (Netherlands)

    Hassena Beko, Mohammed

    2017-01-01

    Ethiopia is an agrarian country where agriculture dominates the economy, and thus agriculture is considered as an engine of growth by the government. Seed as one of the agricultural technologies, in fact, a carrier of many technologies, is critical to increasing production, but the use of quality

  13. Managing Stress. Project Seed.

    Science.gov (United States)

    Muto, Donna; Wilk, Jan

    One of eight papers from Project Seed, this paper describes a stress management project undertaken with high school sophomores. Managing Stress is described as an interactive workshop that offers young people an opportunity to examine specific areas of stress in their lives and to learn effective ways to deal with them. The program described…

  14. Grape Seed Extract

    Science.gov (United States)

    ... Greece people have used grapes, grape leaves, and sap for health purposes. Grape seed extract was developed ... sharing research results, and educating the public. Its resources include publications (such as Dietary ... Department of Health & Human Services, National Institutes of Health, National Center for ...

  15. The SEED Initiative

    Science.gov (United States)

    Teich, Carolyn R.

    2011-01-01

    Committed to fulfilling the promise of the green economy, the American Association of Community Colleges (AACC) launched the Sustainability Education and Economic Development (SEED) initiative (www.theseedcenter.org) in October 2010. The project advances sustainability and clean energy workforce development practices at community colleges by…

  16. Dormancy in Plant Seeds

    NARCIS (Netherlands)

    Hilhorst, H.W.M.; Finch-Savage, W.E.; Buitink, J.; Bolingue, W.; Leubner-Metzger, G.

    2010-01-01

    Seed dormancy has been studied intensely over the past decades and, at present, knowledge of this plant trait is at the forefront of plant biology. The main model species is Arabidopsis thaliana, an annual weed, possessing nondeep physiological dormancy. This overview presents the state-of-the-art

  17. Magnetic-seeding filtration

    Energy Technology Data Exchange (ETDEWEB)

    DePaoli, D.W.; Tsouris, C. [Oak Ridge National Lab., TN (United States); Yiacoumi, Sotira

    1997-10-01

    Magnetic-seeding filtration is a technology under development for the enhanced removal of magnetic and non-magnetic particulates from liquids. This process involves the addition of a small amount of magnetic seed particles (such as naturally occurring iron oxide) to a waste suspension, followed by treatment with a magnetic filter. Non-magnetic and weakly magnetic particles are made to undergo nonhomogeneous flocculation with the seed particles, forming flocs of high magnetic susceptibility that are readily removed by a conventional high-gradient magnetic filter. This technology is applicable to a wide range of liquid wastes, including groundwater, process waters, and tank supernatants. Magnetic-seeding filtration may be used in several aspects of treatment, such as (1) removal of solids, particularly those in the colloidal size range that are difficult to remove by conventional means; (2) removal of contaminants by precipitation processes; and (3) removal of contaminants by sorption processes. Waste stream characteristics for which the technology may be applicable include (1) particle sizes ranging from relatively coarse (several microns) to colloidal particles, (2) high or low radiation levels, (3) broad-ranging flow rates, (4) low to moderate solids concentration, (5) cases requiring high decontamination factors, and (6) aqueous or non-aqueous liquids. At this point, the technology is at the bench-scale stage of development; laboratory studies and fundamental modeling are currently being employed to determine the capabilities of the process.

  18. Magnetic-seeding filtration

    International Nuclear Information System (INIS)

    DePaoli, D.W.; Tsouris, C.; Yiacoumi, Sotira.

    1997-01-01

    Magnetic-seeding filtration is a technology under development for the enhanced removal of magnetic and non-magnetic particulates from liquids. This process involves the addition of a small amount of magnetic seed particles (such as naturally occurring iron oxide) to a waste suspension, followed by treatment with a magnetic filter. Non-magnetic and weakly magnetic particles are made to undergo nonhomogeneous flocculation with the seed particles, forming flocs of high magnetic susceptibility that are readily removed by a conventional high-gradient magnetic filter. This technology is applicable to a wide range of liquid wastes, including groundwater, process waters, and tank supernatants. Magnetic-seeding filtration may be used in several aspects of treatment, such as (1) removal of solids, particularly those in the colloidal size range that are difficult to remove by conventional means; (2) removal of contaminants by precipitation processes; and (3) removal of contaminants by sorption processes. Waste stream characteristics for which the technology may be applicable include (1) particle sizes ranging from relatively coarse (several microns) to colloidal particles, (2) high or low radiation levels, (3) broad-ranging flow rates, (4) low to moderate solids concentration, (5) cases requiring high decontamination factors, and (6) aqueous or non-aqueous liquids. At this point, the technology is at the bench-scale stage of development; laboratory studies and fundamental modeling are currently being employed to determine the capabilities of the process

  19. [Study on seed testing for Salvia miltiorrhiza].

    Science.gov (United States)

    Dan, Hong-mei; Qi, Jian-jun; Zhou, Li-li; Li, Xian-en

    2008-09-01

    To establish a seed testing methods for Salvia miltiorrhiza. Referring to the International Seed Testing Rules made by ISTA and the Seed Testing for Crops (GB/T3543. 1-1995) issued by China. The seeds are selected by winnowing; the seed purity is about 50%-60%; 100 grain weight is used to determine the quality of the seed; the seed moisture content is determined by air drying, the drying hour is 3 h. Seed viability is tested by TFC method.

  20. Germination of red alder seed.

    Science.gov (United States)

    M.A. Radwan; D.S. DeBell

    1981-01-01

    Red alder seeds were collected from six locations throughout the natural range of the species. Each seed lot was obtained from a single tree, and the seeds were used to determine germination with and without stratification treatment. Irrespective of treatment, germination varied significantly (P

  1. Nest-mediated seed dispersal

    Science.gov (United States)

    Robert J. Warren; Jason P. Love; Mark A. Bradford

    2017-01-01

    Many plant seeds travel on the wind and through animal ingestion or adhesion; however, an overlooked dispersal mode may lurk within those dispersal modes. Viable seeds may remain attached or embedded within materials birds gather for nest building. Our objective was to determine if birds inadvertently transport seeds when they forage for plant materials to...

  2. Seed systems support in Kenya

    NARCIS (Netherlands)

    Munyi, Peter; Jonge, De Bram

    2015-01-01

    The threats of climate change and rising food prices have stirred renewed attention for seed and food security in Africa, inviting new thinking on the role of seed sector development in coping with these concerns. One conceptual framework that has gained attention is the Integrated Seed Sector

  3. 7 CFR 201.15 - Weed seeds.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Weed seeds. 201.15 Section 201.15 Agriculture... REGULATIONS Labeling Agricultural Seeds § 201.15 Weed seeds. The percentage of weed seeds shall include seeds of plants considered weeds in the State into which the seed is offered for transportation or...

  4. Epstein-Barr virus nuclear protein 3C binds to the N-terminal (NTD) and beta trefoil domains (BTD) of RBP/CSL; Only the NTD interaction is essential for lymphoblastoid cell growth

    International Nuclear Information System (INIS)

    Calderwood, Michael A.; Lee, Sungwook; Holthaus, Amy M.; Blacklow, Stephen C.; Kieff, Elliott; Johannsen, Eric

    2011-01-01

    Association of EBV nuclear proteins EBNA2, EBNA3A and EBNA3C with RBP/CSL, is essential for lymphoblastoid cell line (LCL) proliferation. Conserved residues in the EBNA3 homology domain, required for RBP/CSL interaction, lack the WΦP motif that mediates EBNA2 and Notch binding to the RBP/CSL beta-trefoil domain (BTD). We map RBP/CSL interacting residues within EBNA3A(aa128-204) and EBNA3C(aa211-233). The EBNA3A results are consistent with an earlier report (aa125-222), but the EBNA3C domain is unexpectedly small and includes a 'WTP' sequence. This EBNA3C WTP motif confers RBP/CSL binding in vitro, in yeast, and in mammalian cells. Further, an EBNA3C WTP → STP(W227S) mutation impaired BTD binding whereas EBNA3 homology domain mutations disrupted RBP/CSL N-terminal domain (NTD) binding. WTP was not essential for EBNA3C repression of EBNA2 in reporter assays or for maintenance of LCL growth. Our results indicate that EBNA3 proteins interact with multiple RBP/CSL domains, but only NTD interactions are required for LCL growth.

  5. Histomorphometric Parameters of the Growth Plate and Trabecular Bone in Wild-Type and Trefoil Factor Family 3 (Tff3)-Deficient Mice Analyzed by Free and Open-Source Image Processing Software.

    Science.gov (United States)

    Bijelić, Nikola; Belovari, Tatjana; Stolnik, Dunja; Lovrić, Ivana; Baus Lončar, Mirela

    2017-08-01

    Trefoil factor family 3 (Tff3) peptide is present during intrauterine endochondral ossification in mice, and its deficiency affects cancellous bone quality in secondary ossification centers of mouse tibiae. The aim of this study was to quantitatively analyze parameters describing the growth plate and primary ossification centers in tibiae of 1-month-old wild-type and Tff3 knock-out mice (n=5 per genotype) by using free and open-source software. Digital photographs of the growth plates and trabecular bone were processed by open-source computer programs GIMP and FIJI. Histomorphometric parameters were calculated using measurements made with FIJI. Tff3 knock-out mice had significantly smaller trabecular number and significantly larger trabecular separation. Trabecular bone volume, trabecular bone surface, and trabecular thickness showed no significant difference between the two groups. Although such histomorphological differences were found in the cancellous bone structure, no significant differences were found in the epiphyseal plate histomorphology. Tff3 peptide probably has an effect on the formation and quality of the cancellous bone in the primary ossification centers, but not through disrupting the epiphyseal plate morphology. This work emphasizes the benefits of using free and open-source programs for morphological studies in life sciences.

  6. Mechanical harvesting of pumpkin seeds

    OpenAIRE

    Sito, Stjepan; Ivančan, Stjepan; Barković, Edi; Mucalo, Ana

    2009-01-01

    One of the key problems in production technology of pumpkin seed for oil production is mechanized harvesting and losses of seed during mechanical harvesting. The losses of pumpkin seed during mechanical harvesting at peripheral velocity of 1.57 m/s (optimally adjusted machine) were 4.4% for Gleisdorf species, 5.2% for Slovenska species and 7.8% for pumpkin with husk. The higher average losses of pumpkin seed with husk were caused by tight connection of seed and pumpkin fruit.

  7. Irradiation effect on the seed vigor, SOD activity and MDA content in germinating seeds of yellow-seeded and black-seeded rape seed (Brassica napus L.)

    International Nuclear Information System (INIS)

    Han Jixiang; Hu Danhong; Liu Houli

    1993-01-01

    Seeds of a set of near-isogenic lines (Brassica napus L.) with different seed coat color from yellow to black were irradiated by 60 Co γ-rays of 150 krad. Seed vigor, superoxide dismutase (SOD) and malondialdehyde (MDA) in germinating seeds were analysed. In these characters, no significant difference between yellow-seeded lines (YLs) and black-seeded lines (BLs) showed before irradiation. But after irradiation, SOD activity in YLs was lower than that in BLs. While MDA content in YLs was obviously higher that that in DLs. As a result of irradiation, seed vigor of YLs was lower than that in BLs. these results indicated that the irradiation resistance of rape seed was related to the level of SOD as well as protective structure or substances in seed coat and that the radiosensitivity of YLs was higher than that of DLs

  8. Breeding for Grass Seed Yield

    DEFF Research Database (Denmark)

    Boelt, Birte; Studer, Bruno

    2010-01-01

    Seed yield is a trait of major interest for many fodder and amenity grass species and has received increasing attention since seed multiplication is economically relevant for novel grass cultivars to compete in the commercial market. Although seed yield is a complex trait and affected...... by agricultural practices as well as environmental factors, traits related to seed production reveal considerable genetic variation, prerequisite for improvement by direct or indirect selection. This chapter first reports on the biological and physiological basics of the grass reproduction system, then highlights...... important aspects and components affecting the seed yield potential and the agronomic and environmental aspects affecting the utilization and realization of the seed yield potential. Finally, it discusses the potential of plant breeding to sustainably improve total seed yield in fodder and amenity grasses....

  9. Physiological quality and seed respiration of primed Jatropha curcas seeds

    Directory of Open Access Journals (Sweden)

    Micheli Angelica Horbach

    2017-11-01

    Full Text Available ABSTRACT Seed deterioration is a natural and irreversible process. Nevertheless, seed priming with water and antioxidants can minimize oxidative damage in oilseeds, resulting in attenuation of seed deterioration. The objective of this assay was to evaluate seed priming on respiratory activity of Jatropha curcas submitted to accelerated aging. Seeds from two provenances (Janauba and Pedro J. Caballero were submitted to three priming treatments (control, immersion in deionized water, and with 750 µmol L-1 of ascorbic acid and treated for accelerated aging at 41 °C for 72 h. The results showed that the priming of J. curcas seeds promoted tolerance to accelerated aging. Primed seeds, with ascorbic acid from Janauba and deionized water from Pedro J. Caballero, resulted in a higher percentage of normal seedlings, and increased germination speed index and seed respiration. The decline of physiological quality of J. curcas seeds after accelerated aging is directly associated with a reduction in respiratory activity that is related to seed moisture content.

  10. DIMENSIONS OF CARCASS AND INTERNAL ORGANS IN YOUNG SHEEP, UNDER CONDITIONS OF GRAZING ON ASSOCIATIONS OF PERENNIAL GRAMINACEOUS AND LEGUME

    Directory of Open Access Journals (Sweden)

    N. DRAGOMIR

    2009-05-01

    Full Text Available The temporary pastures’ floristic structure influences directly the growth and development of young sheep during grazing. The associations, beside the graminaceous and perennial legume species (white clover and birdsfoot trefoil, contribute mostly to the increase of carcass dimension and organ weight. The quality of the forage from such a pasture, better balanced in terms of energy and protein, influences all growth and development parameters in young sheep.

  11. Empty seeds are not always bad: simultaneous effect of seed emptiness and masting on animal seed predation.

    Directory of Open Access Journals (Sweden)

    Ramón Perea

    Full Text Available Seed masting and production of empty seeds have often been considered independently as different strategies to reduce seed predation by animals. Here, we integrate both phenomena within the whole assemblage of seed predators (both pre and post-dispersal and in two contrasting microsites (open vs. sheltered to improve our understanding of the factors controlling seed predation in a wind-dispersed tree (Ulmus laevis. In years with larger crop sizes more avian seed predators were attracted with an increase in the proportion of full seeds predated on the ground. However, for abundant crops, the presence of empty seeds decreased the proportion of full seeds predated. Empty seeds remained for a very long period in the tree, making location of full seeds more difficult for pre-dispersal predators and expanding the overall seed drop period at a very low cost (in dry biomass and allocation of C, N and P. Parthenocarpy (non-fertilized seeds was the main cause of seed emptiness whereas seed abortion was produced in low quantity. These aborted seeds fell prematurely and, thus, could not work as deceptive seeds. A proportion of 50% empty seeds significantly reduced ground seed predation by 26%. However, a high rate of parthenocarpy (beyond 50% empty seeds did not significantly reduce seed predation in comparison to 50% empty seeds. We also found a high variability and unpredictability in the production of empty seeds, both at tree and population level, making predator deception more effective. Open areas were especially important to facilitate seed survival since rodents (the main post-dispersal predators consumed seeds mostly under shrub cover. In elm trees parthenocarpy is a common event that might work as an adaptive strategy to reduce seed predation. Masting per se did not apparently reduce the overall proportion of seeds predated in this wind-dispersed tree, but kept great numbers of seeds unconsumed.

  12. Seeds of the Future

    CERN Multimedia

    CERN. Geneva

    2015-01-01

    Five of the global issues most frequently debated today are the decline of biodiversity in general and of agrobiodiversity in particular, climate change, hunger and malnutrition, poverty and water. These issues are connected with each other, and should be dealt with as such. Most of our food comes from seeds (even when we eat meat, we indirectly eat plants, which come from seeds) and food affects our health. The evolution of plant breeding, the science which is responsible for the type and the diversity of seed that farmers plant, and hence for the diversity of food that we eat, helps us understand how agrobiodiversity has decreased. An agro-ecological model of agriculture could be solution to the most important problems affecting the planet, but is often criticized for not being able to produce enough food for a growing population casting doubts on whether food security and food safety can be compatible objectives. Participatory and evolutionary plant breeding, while benefiting from advances in molecular g...

  13. Physicochemical Evaluation of Seeds and Oil of Nontraditional Oil Seeds

    Directory of Open Access Journals (Sweden)

    Adam Ismail Ahmed

    2015-08-01

    Full Text Available The present work was conducted in the Laboratory of Biochemistry and Food science department, Faculty of Natural Resources and Environmental Studies, University of Kordofan, in order to evaluate some nontraditional oil seeds these are i.e. Marula (Sclerocarya birrea, Roselle (Hibiscus sabdariffa L. seeds and Christ’s thorn (Zizyphus spina-christi seeds. The seeds of the roselle and Christ’s thorn fruits were procured from Elobeid local market, North Kordofan State, while marula fruits were obtained from Elnuhod, West Kordofan State. The proximate composition of the seeds, cake and christ’s thorn pulp was done. Some chemical and physical properties were performed for the extracted oil. The results revealed that proximate composition of the seeds and cake differ statistically among the studied materials. Significant differences were observed among the oil extracted from these species; moreover, these oils differ significantly in color and viscosity only.

  14. Sunflower seed allergy

    Science.gov (United States)

    Ukleja-Sokołowska, Natalia; Gawrońska-Ukleja, Ewa; Żbikowska-Gotz, Magdalena; Bartuzi, Zbigniew; Sokołowski, Łukasz

    2016-01-01

    Sunflower seeds are a rare source of allergy, but several cases of occupational allergies to sunflowers have been described. Sunflower allergens on the whole, however, still await precise and systematic description. We present an interesting case of a 40-year-old male patient, admitted to hospital due to shortness of breath and urticaria, both of which appeared shortly after the patient ingested sunflower seeds. Our laryngological examination revealed swelling of the pharynx with retention of saliva and swelling of the mouth and tongue. During diagnostics, 2 months later, we found that skin prick tests were positive to mugwort pollen (12/9 mm), oranges (6/6 mm), egg protein (3/3 mm), and hazelnuts (3/3 mm). A native prick by prick test with sunflower seeds was strongly positive (8/5 mm). Elevated concentrations of specific IgE against weed mix (inc. lenscale, mugwort, ragweed) allergens (1.04 IU/mL), Artemisia vulgaris (1.36 IU/mL), and Artemisia absinthium (0.49 IU/mL) were found. An ImmunoCap ISAC test found an average level of specific IgE against mugwort pollen allergen component Art v 1 - 5,7 ISU-E, indicating an allergy to mugwort pollen and low to medium levels of specific IgE against lipid transfer proteins (LTP) found in walnuts, peanuts, mugwort pollen, and hazelnuts. Through the ISAC inhibition test we proved that sunflower seed allergen extracts contain proteins cross-reactive with patients’ IgE specific to Art v 1, Art v 3, and Jug r 3. Based on our results and the clinical pattern of the disease we confirmed that the patient is allergic to mugwort pollen and that he had an anaphylactic reaction as a result of ingesting sunflower seeds. We suspected that hypersensitivity to sunflower LTP and defensin-like proteins, both cross-reactive with mugwort pollen allergens, were the main cause of the patient’s anaphylactic reaction. PMID:27222528

  15. Pathogenic mycoflora on carrot seeds

    Directory of Open Access Journals (Sweden)

    Bogdan Nowicki

    2013-12-01

    Full Text Available Altogether 300 seed samples were collected during 9 years in 8 regions of Poland and the fungi Were isolated and their pathogenicity to carrot seedlings was examined. Alternaria rudicina provcd to be the most important pathogen although. A. alternata was more common. The other important pathogens were Fusarium spp., Phoma spp. and Botrytis cinerea. The infection of carrot seeds by A. radicina should be used as an important criterium in seed quality evaluation.

  16. Maturation of sugar maple seed

    Science.gov (United States)

    Clayton M., Jr. Carl; Albert G., Jr. Snow; Albert G. Snow

    1971-01-01

    The seeds of a sugar maple tree (Acer saccharum Marsh.) do not mature at the same time every year. And different trees mature their seeds at different times. So time of year is not a reliable measure of when seeds are ripe. Better criteria are needed. In recent studies we have found that moisture content and color are the best criteria for judging when sugar maple...

  17. Within canopy distribution of cotton seed N

    Science.gov (United States)

    Whole cotton seeds can be an important component of dairy rations. Nitrogen content of the seed is an important determinant of the feed value of the seed. Efforts to increase the seed value as feed will be enhanced with knowledge of the range and distribution of seed N within the cotton crop. This s...

  18. A role for seed storage proteins in Arabidopsis seed longevity

    NARCIS (Netherlands)

    Nguyen, Thu-Phuong|info:eu-repo/dai/nl/328228818; Cueff, Gwendal; Hegedus, Dwayne D; Rajjou, Loïc; Bentsink, Leónie|info:eu-repo/dai/nl/241338735

    2015-01-01

    Proteomics approaches have been a useful tool for determining the biological roles and functions of individual proteins and identifying the molecular mechanisms that govern seed germination, vigour and viability in response to ageing. In this work the dry seed proteome of four Arabidopsis thaliana

  19. 19 CFR 10.57 - Certified seed potatoes, and seed corn or maize.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Certified seed potatoes, and seed corn or maize... Provisions Potatoes, Corn, Or Maize § 10.57 Certified seed potatoes, and seed corn or maize. Claim for classification as seed potatoes under subheading 0701.10.00, as seed corn (maize) under subheading 1005.10...

  20. Seed dormancy and seed longevity: from genetic variation to gene identification

    NARCIS (Netherlands)

    Nguyen, T.P.

    2014-01-01

    Seed dormancy and seed longevity are the most important survival traits in the soil seed bank. Both traits are induced during seed maturation and evolved to assure seed survival during environmental conditions that cannot support the regular course of life. Seed dormancy is related to the timing of

  1. Why high seed densities within buried mesh bags may overestimate depletion rates of soil seed banks

    NARCIS (Netherlands)

    Mourik, van T.A.; Stomph, T.J.; Murdoch, A.J.

    2005-01-01

    1. Estimates of seed bank depletion rates are essential for modelling and management of plant populations. The seed bag burial method is often used to measure seed mortality in the soil. However, the density of seeds within seed bags is higher than densities in natural seed banks, which may elevate

  2. Heirloom biodynamic seeds network rescue, conservation and multiplication of local seeds in Brazil

    OpenAIRE

    Jovchelevich, Pedro

    2014-01-01

    Structuring a network organic and biodynamic seed involving farmers in the central- southern Brazil. Training, participatory breeding, edition of publications, fairs of exchange seeds, a processing unit and assessment of seed quality, commercial seed multiplication with emphasis on vegetables. This network has garanteed the autonomy of farmers in seed production and enriched agrobiodiversity through exchanges of seed.

  3. Seed drill depth control system for precision seeding

    DEFF Research Database (Denmark)

    Kirkegaard Nielsen, Søren; Munkholm, Lars Juhl; Lamandé, Mathieu

    2018-01-01

    acting on the drill coulters, which generates unwanted vibrations and, consequently, a non-uniform seed placement. Therefore, a proof-of-concept dynamic coulter depth control system for a low-cost seed drill was developed and studied in a field experiment. The performance of the active control system...... depth control system this variability was reduced to±2 mm. The system with the active control system operated more accurately at an operational speed of 12 km h−1 than at 4 km h−1 without the activated control system.......An adequate and uniform seeding depth is crucial for the homogeneous development of a crop, as it affects time of emergence and germination rate. The considerable depth variations observed during seeding operations - even for modern seed drills - are mainly caused by variability in soil resistance...

  4. 7 CFR 201.50 - Weed seed.

    Science.gov (United States)

    2010-01-01

    ... REGULATIONS Purity Analysis in the Administration of the Act § 201.50 Weed seed. Seeds (including bulblets or... sieve are considered weed seeds. For wild onion and wild garlic (Allium spp.) bulblets classed as inert...

  5. Seeding and planting upland oaks

    Science.gov (United States)

    1989-01-01

    Oaks can be planted or seeded in uplands to: (1) afforest old fields, strip-mined areas, or other areas devoid of trees, and (2) supplement natural reproduction within existing forests. Planting is usually more successful than direct seeding. But even under good conditions survival and growth of planted oak has been considerably poorer than with conifers and other...

  6. STORAGE OF Handroanthus umbellatus SEEDS

    Directory of Open Access Journals (Sweden)

    Cibele Chalita Martins

    2014-09-01

    Full Text Available http://dx.doi.org/10.5902/1980509815725Seed storage under controlled environmental conditions represents one of the most important lines of research to be applied on short-lived forest species as Handroanthus. The present research aimed to identify the most suitable seed storage conditions and longevity behavior of Handroanthus umbellatus seeds subject to the following storage treatments: packaging permeable paper bags under a no-controlled laboratory temperature and humidity (control and multiwall semipermeable bag at temperatures of -18 ºC, 1 ºC and 25 ºC. Seeds were dried to 6.3% of water content. Stored seeds were evaluated every three months until 24 months for water content, germination percentage and vigor utilizing first counting test. Seeds of T. umbellata are orthodox, with low longevity under natural conditions, once they remain viable for less than 5 months. The best conditions of seed preservation of these species were obtained by storage at -18° C in multiwall bags. Under these conditions physiological seed quality remains unchanged for a 24-month period.

  7. Uganda Early Generation Seed Study

    NARCIS (Netherlands)

    Mastenbroek, A.; Ntare, Bonny

    2016-01-01

    One of the major bottlenecks limiting farmers’ access to good quality seed for food crops in Uganda is the shortage of early generation seed (EGS - breeder and foundation) to produce sufficient quantities of certified and/or quality declared) to satisfy the needs of farmers. A national study was

  8. Insecticide seed treatments for sugarbeet

    Science.gov (United States)

    Pest feeding and vectoring of viruses cause serious problems in sugarbeet production worldwide. In order to ameliorate pest and disease problems on sugarbeet, two seed treatments, Poncho Beta (60 g a.i. clothianidin + 8 g a.i. beta-cyfluthrin/100,000 seed) and Cruiser Tef (60 g a.i. thiamethoxam + 8...

  9. Orthodox seeds and resurrection plants

    NARCIS (Netherlands)

    Costa, Maria Cecília Dias; Cooper, Keren; Hilhorst, Henk W.M.; Farrant, Jill M.

    2017-01-01

    Although staple crops do not survive extended periods of drought, their seeds possess desiccation tolerance (DT), as they survive almost complete dehydration (desiccation) during the late maturation phase of development. Resurrection plants are plant species whose seeds and vegetative tissues are

  10. Efficient computation of spaced seeds

    Directory of Open Access Journals (Sweden)

    Ilie Silvana

    2012-02-01

    Full Text Available Abstract Background The most frequently used tools in bioinformatics are those searching for similarities, or local alignments, between biological sequences. Since the exact dynamic programming algorithm is quadratic, linear-time heuristics such as BLAST are used. Spaced seeds are much more sensitive than the consecutive seed of BLAST and using several seeds represents the current state of the art in approximate search for biological sequences. The most important aspect is computing highly sensitive seeds. Since the problem seems hard, heuristic algorithms are used. The leading software in the common Bernoulli model is the SpEED program. Findings SpEED uses a hill climbing method based on the overlap complexity heuristic. We propose a new algorithm for this heuristic that improves its speed by over one order of magnitude. We use the new implementation to compute improved seeds for several software programs. We compute as well multiple seeds of the same weight as MegaBLAST, that greatly improve its sensitivity. Conclusion Multiple spaced seeds are being successfully used in bioinformatics software programs. Enabling researchers to compute very fast high quality seeds will help expanding the range of their applications.

  11. Kauri seeds and larval somersaults

    DEFF Research Database (Denmark)

    Dupont, Steen Thorleif

    2012-01-01

    The trunk morphology of the larvae of the kauri pine (Agathis) seed infesting moth Agathiphaga is described using conventional, polarization, and scanning electron microscopy. The pine seed chamber formed by the larva is also described and commented on. The simple larval chaetotaxy includes more ...

  12. Banking Wyoming big sagebrush seeds

    Science.gov (United States)

    Robert P. Karrfalt; Nancy Shaw

    2013-01-01

    Five commercially produced seed lots of Wyoming big sagebrush (Artemisia tridentata Nutt. var. wyomingensis (Beetle & Young) S.L. Welsh [Asteraceae]) were stored under various conditions for 5 y. Purity, moisture content as measured by equilibrium relative humidity, and storage temperature were all important factors to successful seed storage. Our results indicate...

  13. Revegetation and survey of vegetation transition of repaired old shotcrete slope; Rokyukashita morutaru fukitsuke norimen no ryokka

    Energy Technology Data Exchange (ETDEWEB)

    Yokota, H.; Deguchi, C. [Miyazaki Univ., Miyazaki (Japan). Faculty of Engineering; Yakabe, H. [Dia Consultants Co. Ltd., Tokyo (Japan); Matsuura, H.

    1996-06-01

    The National road No.220 run from Miyazaki city to Kagoshima city mostly along the seashore and has many slopes by the roadside composed of mudstone which is apt to weather. Although renewal of slopes which were shotcreted for reinforcement around 1970 are coming into question, repair works accompanied with vegetation transition have to be required from the viewpoint of natural environmental management. A part of a slope (9.6 {times} 15m) offered for experiment had been reinforced by RC frame and this time was reshotcreted. Each of eighty compartments surrounded by frames was made into a flower bed respectively having a porous board at the bottom which was 5,10 or 15cm in thickness. And also some vegetation boxes were prepared partially. At the time when just two years have passed from seeding (Dec.1993), in pastures, Bermuda grass and Jaguar the 2nd., in flowers, coreopsis and in the vegetation boxes bird`s-foot trefoil and vitex rotundifolia have grown well. On the other conventional slope, oenothera odorata, chrysanthemum japonense and pampas grass were grown spontaneously, which are considered as hopeful grasses in future. 4 refs., 2 figs., 4 tabs.

  14. Vigor of sunflower and soybean aging seed

    OpenAIRE

    Tatić M.; Balešević-Tubić S.; Ðorđević V.; Miklič V.; Vujaković M.; Ðukić V.

    2012-01-01

    Seed aging and deterioration affect seed vigor and viability. The characteristics of the chemical composition of oil crops seed are related to specific processes occurring in the seed during storage. This study was performed to examine the changes in seed vigor of different sunflower and soybean genotypes under controlled and conventional (uncontrolled) conditions of natural aging for six and twelve months. Obtained results show that the degree of seed dama...

  15. Wheat seed system in Ethiopia: Farmers' varietal perception, seed sources, and seed management

    NARCIS (Netherlands)

    Bishaw, Z.; Struik, P.C.; Gastel, van A.J.G.

    2010-01-01

    Knowledge and information on farmers' perception and its influence on adoption of modern wheat varieties, awareness and source of new wheat production technology, wheat seed sources, and on-farm seed-management practices remain sporadic in Ethiopia. This study was conducted to understand the

  16. Farmer’s seed sources and seed quality: 2. seed health

    NARCIS (Netherlands)

    Bishaw, Z.; Struik, P.C.; Gastel, van A.J.G.

    2013-01-01

    The study assessed the health quality of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) seed samples collected from formal and informal sector in Ethiopia and Syria. In Ethiopia, several seed-borne fungi were found on wheat samples: Cochliobolus sativum, Fusarium avenaceum, F.

  17. Size, physiological quality, and green seed occurrence influenced by seeding rate in soybeans

    Directory of Open Access Journals (Sweden)

    André Sampaio Ferreira

    2017-05-01

    Full Text Available The seeding rate influences the intraspecific competition, which might affect the development and quality of seeds in soybean. However, the impact of seeding rate on the physical and physiological qualities of soybean seeds needs to be better elucidated. This study aimed to evaluate the effects of soybean plant density on the seed size as well as the effects of the interaction between the plant density and seed size on the seed mass, green seed occurence, and physiological seed quality. The experiments were carried out in the growing seasons of the years 2013/14 and 2014/15 in a Latossolo Vermelho distroférrico, under a randomized complete block design, using the NK 7059 RR cultivar with six replications. Four plant densities (150, 300, 440, and 560 thousand viable seeds ha–1 were evaluated. After the classification of seeds into four sizes, using a set of sieves, a 4 ×4 factorial scheme was used for the statistical analysis of the four plant densities and four seed sizes. The seed samples were evaluated for the seed mass, green seed percentage, germination, and vigor. Under thermal and water stress during seed development, an increase in the seeding rate led to a reduction in the green seed occurrence and an increase in the seed size and mass. However, in the absence of thermal and water stress, the seed size and mass were not altered by the seeding rate and, there was no occurrence of green seeds.

  18. Wheat and barley seed systems in Ethiopia and Syria

    NARCIS (Netherlands)

    Bishaw, Z.

    2004-01-01

    Keywords: Wheat,Triticumspp., Barley,Hordeumvulgare L., Seed Systems, Formal Seed Sector, Informal Seed Sector, National Seed Program, Seed Source, Seed Selection, Seed Management, Seed Quality,

  19. A system for generating virtual seeds

    Directory of Open Access Journals (Sweden)

    Sako Y.

    1998-01-01

    Full Text Available Seed analysts need to identify seeds, and seed catalogs are used as a reference to accomplish this task. Conventional seed catalogs supply two-dimensional photographs and hand-drawn diagrams. In this study, a new, three-dimensional representation of seeds is developed to supplement these traditional photographs and drawings. QuickTime VR is a promising method for viewing three-dimensional objects on a computer screen. It permits manipulation of an object by rotating and viewing it from any pre-specified angle at an interactive speed, allowing the viewer the sense of examining a hand-held object. In this study, QuickTime VR object movies of seeds were created as interactive "movies" of seeds that can be rotated and scaled to give the viewer the sensation of examining actual seeds. This approach allows the examination of virtual seeds from any angle, permitting more accurate identification of seeds by seed analysts.

  20. Seed selection by earthworms : chemical seed properties matter more than morphological traits

    OpenAIRE

    Clause, J.; Forey, E.; Eisenhauer, N.; Seal, C.E.; Soudey, A.; Colville, L.; Barot, Sébastien

    2017-01-01

    Aims : The passage of seeds through the earthworm gut potentially damages seeds, altering seed and seedling performances depending on seed traits. This work was conducted to study to what extent chemical and morphological seed traits determine the seed attractiveness for earthworms. Methods : We tested seed selection via the ingestion and digestion of 23 grassland plant species spanning a range of 14 morphological and chemical traits by two common earthworm species: the anecic Lumbricus te...

  1. Effects of seed fermentation method on seed germination and vigor ...

    African Journals Online (AJOL)

    BERTIN

    2013-11-27

    Nov 27, 2013 ... methods (fermented in ambient air, plastic bag stored in ambient or in plastic bag buried) were tested ... fruits into plastic bag that was exposed in ambient air in the field; SFD, seeds ..... Concepts and technologies of selected.

  2. Improving the traditional sesame seed planting with seed pelleting

    African Journals Online (AJOL)

    PRECIOUS

    2009-11-16

    Nov 16, 2009 ... 1Department of Agricultural Machinery, Faculty of Agriculture, Adnan Menderes University, Turkey. 2Fethiye ... the pelleted sesame seeds improved the yield significantly ... Sesame holds a special importance in the world's oil.

  3. Laser treatment of radish seed

    International Nuclear Information System (INIS)

    Kartalov, P.; Nidal, T.

    1987-01-01

    Trials were conducted in unheated plastic greenhouses in 1985-1986 to test the effect of laser treatment on radish seed. Seed of cv Saxia was irradiated with helio-neon laser of 632.8 Nm wave length at: 2-, 4- and 6-fold irradiation. Results showed that plants of all variants emerged almost simultaneously. The root mass was greatest for plants obtained from 4-fold irradiated seed. Treatment enhanced root production in 1985, and in 1986 4-fold irradiation boosted yield by 15%

  4. Studies on the Vigour of Soybean Seeds : II. Varietal Differences in Seed Coat Quality and Swelling Components of Seed during Moisture Imbibition

    OpenAIRE

    Mugnisjah, Wahju Qamara; Shimano, Itaru; Matsumoto, Shigeo; 島野, 至; 松本, 重男

    1987-01-01

    Laboratory experiment was conducted to elucidate the physiological factor determining the association of seed size and seed coat quality with varietal differences in seed vigour, and to pursue an alternative on the mechanism of varietal differences in seed resistance to field weathering. Results of this study revealed that seed polymer change (seed volume change minus seed weight change during moisture imbibition) was the physiological factor determining the association of seed size and seed ...

  5. Seed cryopreservation of Fraxinus angustifolia Vahl

    African Journals Online (AJOL)

    Università

    2013-04-17

    control) and liquid nitrogen-treated seeds were .... 76.0 ± 4.0 (NT in GA3), with an intermediary value of 70.0. ± 5.0% (Cryo seeds in water .... in November, suggesting a loss of dormancy during seed maturation. Furthermore, seed ...

  6. Storage requirements for sugar maple seeds

    Science.gov (United States)

    Harry W. Yawney; Clayton M., Jr. Carl

    1974-01-01

    Sugar maple seeds, collected from three trees in northern Vermont, were stored at four temperatures (18, 7, 2, and -10ºC) in combination with four seed moisture contents (35, 25, 17, and 10 percent). Seed moisture content and storage temperature significantly affected keeping ability, and these factors were highly interrelated. Seeds from all trees kept best...

  7. 7 CFR 948.6 - Seed potatoes.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Seed potatoes. 948.6 Section 948.6 Agriculture... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE IRISH POTATOES GROWN IN COLORADO Order Regulating Handling Definitions § 948.6 Seed potatoes. Seed potatoes or seed means any potatoes...

  8. 7 CFR 201.57 - Hard seeds.

    Science.gov (United States)

    2010-01-01

    ... REGULATIONS Germination Tests in the Administration of the Act § 201.57 Hard seeds. Seeds which remain hard at the end of the prescribed test because they have not absorbed water, due to an impermeable seed coat... percentage of germination. For flatpea, continue the swollen seed in test for 14 days when germinating at 15...

  9. Brassica rapa L. seed development in hypergravity

    NARCIS (Netherlands)

    Musgrave, M.E.; Kuang, A.; Allen, J.; Blasiak, J.; van Loon, J.J.W.A.

    2009-01-01

    Previous experiments had shown that microgravity adversely affected seed development in Brassica rapa L. We tested the hypothesis that gravity controls seed development via modulation of gases around the developing seeds, by studying how hypergravity affects the silique microenvironment and seed

  10. Evaluation of Lettuce Genotypes for Seed Thermotolerance

    Science.gov (United States)

    Thermoinhibition of lettuce (Lactuca sativa L.) seed germination is a common problem associated with lettuce production. Depending on lettuce cultivars, seed germination may be inhibited when temperatures exceed 28oC. The delay or inhibition of seed germination at high temperatures may reduce seedli...

  11. Analysis of seed quality in NS sunflower hybrid seed processed between 2010 and 2014

    Directory of Open Access Journals (Sweden)

    Jokić Goran

    2015-01-01

    Full Text Available This paper analyzed the processed seed of five sunflower hybrid seed developed at the Institute of Field and Vegetable Crops in Novi Sad. The cultivars were Rimi PR, Duško, NS Dukat, Sumo 1 PR and Sremac. The analysis was conducted on seed lots processed between 2010 and 2014 and involved the following parameters: seed purity percentage, 1000-seed weight, germination energy, germination, seed moisture, number of weed seeds per 1000 grams of seed. The results of the study produced the following average values: seed purity - 99.72%, 1000-seed weight - 67.59g, germination energy - 88.2%, germination - 91.8%, seed moisture - 8.3%. There were not found weeds seeds as well as pathogens on the seed samples, these values are all within the legally prescribed limits.

  12. Autoradiography for iodine-125 seeds

    International Nuclear Information System (INIS)

    Alberti, W.; Divoux, S.; Pothmann, B.; Tabor, P.; Hermann, K.P.; Harder, D.

    1993-01-01

    To study the interior design of model 6702 and 6711 iodine-125 seeds, contact autoradiographs were performed using mammography film. Improved resolution was obtained using a pin-hole camera with a hole of 0.1 mm x 0.1 mm. With these techniques, qualitative determination of the relative activity distribution within each seed was possible. The number of the activated resin spheres and the positions of the centers of these spheres can be exactly determined. A model calculation shows that variations in the arrangement of the activated spheres within a seed have a moderate influence on the dose distribution at source distances below 10 mm. Knowing the exact source configuration may be useful when comparing dose calculations with measured data for model 6702 125 I seeds which are currently employed in ophthalmic plaque and implant therapy of other tumors. 16 refs., 5 figs., 2 tabs

  13. Ethylene, seed germination, and epinasty.

    Science.gov (United States)

    Stewart, E R; Freebairn, H T

    1969-07-01

    Ethylene activity in lettuce seed (Lactuca satina) germination and tomato (Lycopersicon esculentum) petiole epinasty has been characterized by using heat to inhibit ethylene synthesis. This procedure enabled a separation of the production of ethylene from the effect of ethylene. Ethylene was required in tomato petioles to produce the epinastic response and auxin was found to be active in producing epinasty through a stimulation of ethylene synthesis with the resulting ethylene being responsible for the epinasty. In the same manner, it was shown that gibberellic acid stimulated ethylene synthesis in lettuce seeds. The ethylene produced then in turn stimulated the seeds to germinate. It was hypothesized that ethylene was the intermediate which caused epinasty or seed germination. Auxin and gibberellin primarily induced their response by stimulating ethylene production.

  14. Lack of caching of direct-seeded Douglas fir seeds by deer mice

    International Nuclear Information System (INIS)

    Sullivan, T.P.

    1978-01-01

    Seed caching by deer mice was investigated by radiotagging seeds in forest and clear-cut areas in coastal British Columbia. Deer mice tend to cache very few Douglas fir seeds in the fall when the seed is uniformly distributed and is at densities comparable with those used in direct-seeding programs. (author)

  15. Seed mass and mast seeding enhance dispersal by a neotropical scatter-hoarding rodent

    NARCIS (Netherlands)

    Jansen, P.A.; Bongers, F.J.J.M.; Hemerik, L.

    2004-01-01

    Many tree species that depend on scatter-hoarding animals for seed dispersal produce massive crops of large seeds at irregular intervals. Mast seeding and large seed size in these species have been explained as adaptations to increase animal dispersal and reduce predation. We studied how seed size

  16. Chloroxyanion residue on seeds and sprouts after chlorine dioxide sanitation of alfalfa seed

    Science.gov (United States)

    The effects of a 6-h chlorine dioxide sanitation of alfalfa seed (0, 50, 100, and 200 mg/kg seed) on total coliform bacteria, seed germination, and on the presence of chlorate and perchlorate residues in seed rinse, seed soak, and in alfalfa sprouts was determined. Chlorate residues in 20000 ppm cal...

  17. Moringa Seed Oils

    Directory of Open Access Journals (Sweden)

    Joana O. Ilesanmi

    2010-01-01

    Full Text Available This study was conducted to determine the effects of neem (Azadirachta indica A. Juss and moringa (Moringa oleifera seed oils on the storability of cowpea grain. Cowpea samples were treated with various concentrations (0.5, 1.0, and 1.5 mL/200 g cowpea of pure neem and moringa oils and their mixtures in ratios of 1:1, 1:2, and 1:3. The treated cowpea samples were stored for 180 days. Data were collected every 30 days on number of eggs laid, total weevil population, and percentage of uninfested grains and analysed statistically. Significantly different means were compared using LSD at <.05. Increasing oil concentration resulted in better cowpea protection, for example, in oviposition where the control had 6513 eggs, only 8 eggs were recorded in pure neem oil-treated sample at 0.5 mL/200 g. Generally, better results were obtained with higher oil concentrations either in their pure forms or mixtures. The control had a total weevil population of 4988, while most treated samples had none. The control samples had 0% uninfested grains, while 73–94% of uninfested grains were observed in treated samples after 6 months of storage. Therefore, mixture of the oils at 1.5 mL/200 g can be effectively used to store cowpea.

  18. Diamond Synthesis Employing Nanoparticle Seeds

    Science.gov (United States)

    Uppireddi, Kishore (Inventor); Morell, Gerardo (Inventor); Weiner, Brad R. (Inventor)

    2014-01-01

    Iron nanoparticles were employed to induce the synthesis of diamond on molybdenum, silicon, and quartz substrates. Diamond films were grown using conventional conditions for diamond synthesis by hot filament chemical vapor deposition, except that dispersed iron oxide nanoparticles replaced the seeding. This approach to diamond induction can be combined with dip pen nanolithography for the selective deposition of diamond and diamond patterning while avoiding surface damage associated to diamond-seeding methods.

  19. Seed governance. From seed aid to seed system security in fragile areas

    NARCIS (Netherlands)

    Rietberg, P.I.; Gevers, H.; Hospes, O.

    2014-01-01

    Intergovernmental agencies and development organizations, including Cordaid, consider interventions directed at seed security of utmost importance to support smallholders recovering from conflict situations and disasters, and to contribute to revitalisation of local agricultural production and food

  20. Optimum harvest maturity for Leymus chinensis seed

    Directory of Open Access Journals (Sweden)

    Jixiang Lin

    2016-06-01

    Full Text Available Timely harvest is critical to achieve maximum seed viability and vigour in agricultural production. However, little information exists concerning how to reap the best quality seeds of Leymus chinensis, which is the dominant and most promising grass species in the Songnen Grassland of Northern China. The objective of this study was to investigate and evaluate possible quality indices of the seeds at different days after peak anthesis. Seed quality at different development stages was assessed by the colours of the seed and lemmas, seed weight, moisture content, electrical conductivity of seed leachate and germination indices. Two consecutive years of experimental results showed that the maximum seed quality was recorded at 39 days after peak anthesis. At this date, the colours of the seed and lemmas reached heavy brown and yellow, respectively. The seed weight was highest and the moisture content and the electrical conductivity of seed leachate were lowest. In addition, the seed also reached its maximum germination percentage and energy at this stage, determined using a standard germination test (SGT and accelerated ageing test (AAT. Thus, Leymus chinensis can be harvested at 39 days after peak anthesis based on the changes in parameters. Colour identification can be used as an additional indicator to provide a more rapid and reliable measure of optimum seed maturity; approximately 10 days after the colour of the lemmas reached yellow and the colour of the seed reached heavy brown, the seed of this species was suitable for harvest.

  1. Phenolics in the seed coat of wild soybean (Glycine soja) and their significance for seed hardness and seed germination.

    Science.gov (United States)

    Zhou, San; Sekizaki, Haruo; Yang, Zhihong; Sawa, Satoko; Pan, Jun

    2010-10-27

    Hardseededness in annual wild soybean (Glycine soja Sieb. Et Zucc.) is a valuable trait that affects the germination, viability, and quality of stored seeds. Two G. soja ecotypes native to Shandong Province of China have been used to identify the phenolics in the seed coat that correlate with the seed hardness and seed germination. Three major phenolics from the seed coat were isolated and identified as epicatechin, cyanidin 3-O-glucoside, and delphinidin 3-O-glucoside. Of the three phenolics, only the change of epicatechin exhibited a significant positive correlation with the change of hard seed percentages both under different water conditions during seed development and under different gas conditions during seed storage. Epicatechin also reveals a hormesis-like effect on the seed germination of G. soja. Epicatechin is suggested to be functionally related to coat-imposed hardseededness in G. soja.

  2. Equilibrium relative humidity as a tool to monitor seed moisture

    Science.gov (United States)

    Robert P. Karrfalt

    2010-01-01

    The importance of seed moisture in maintaining high seed viability is well known. The seed storage chapters in the Tropical Tree Seed Manual (Hong and Ellis 2003) and the Woody Plant Seed Manual (Bonner 2008a) give a detailed discussion and many references on this point. Working with seeds in an operational setting requires a test of seed moisture status. It is...

  3. The Seed Proteome Web Portal

    Directory of Open Access Journals (Sweden)

    Marc eGalland

    2012-06-01

    Full Text Available The Seed Proteome Web Portal (SPWP; http://www.seedproteome.com/ gives access to information both on quantitative seed proteomic data and on seed-related protocols. Firstly, the SPWP provides access to the 475 different Arabidopsis seed proteins annotated from 2 dimensional electrophoresis (2DE maps. Quantitative data are available for each protein according to their accumulation profile during the germination process. These proteins can be retrieved either in list format or directly on scanned 2DE maps. These proteomic data reveal that 40% of seed proteins maintain a stable abundance over germination, up to radicle protrusion. During sensu stricto germination (24 h upon imbibition about 50% of the proteins display quantitative variations, exhibiting an increased abundance (35% or a decreasing abundance (15%. Moreover, during radicle protrusion (24 h to 48 h upon imbibition, 41% proteins display quantitative variations with an increased (23% or a decreasing abundance (18%. In addition, an analysis of the seed proteome revealed the importance of protein post-translational modifications as demonstrated by the poor correlation (r2 = 0.29 between the theoretical (predicted from Arabidopsis genome and the observed protein isoelectric points. Secondly, the SPWP is a relevant technical resource for protocols specifically dedicated to Arabidopsis seed proteome studies. Concerning 2D electrophoresis, the user can find efficient procedures for sample preparation, electrophoresis coupled with gel analysis and protein identification by mass spectrometry, which we have routinely used during the last 12 years. Particular applications such as the detection of oxidized proteins or de novo synthetized proteins radiolabeled by [35S]-methionine are also given in great details. Future developments of this portal will include proteomic data from studies such as dormancy release and protein turnover through de novo protein synthesis analyses during germination.

  4. Report on seed born diseases in organic seed and propagation material

    OpenAIRE

    Micheloni, C.; Plakolm, G.; Schärer, H.

    2007-01-01

    The key questions which will be addressed in this report are: • Are seed born diseases an important factor that prevents seed companies from producing organic seeds and organic farmers from using them? • Which seed treatments are available in organic farming? Which treatments are or will be acceptable? To which degree are they effective? • Are the thresholds for seed born diseases different among Member States? Can this cause unfair competition among farmers and seed producers? • ...

  5. Influence of Fungicides Application and Seed Processing on Sunflower Seed Quality

    OpenAIRE

    Šimić, Branimir; Svitlica, Brankica; Ćosić, Jasenka; Andrić, Luka; Rozman, Vlatka; Postić, Jelena; Liović, Ivica

    2009-01-01

    The aim of the research was to determine influence of methods of seed processing and application of fungicides on mass of 1000 seeds, intergrowth energy and seed germination of sunflower hybrids ‘Favorit’ and ‘Apolon’ in 2005 and 2006. Selected untreated seeds of both sunflower hybrids (control) had a minimal mass of 1000 seeds (55-59 g), intergrowth energy (18-37 %) and seed germination (39-52 %). In both years of research maximal seed quality, mass of 1000 seeds (67-69 g), intergrowth energ...

  6. Neutron irradiation of seeds 2

    Energy Technology Data Exchange (ETDEWEB)

    1968-10-01

    The irradiation of seeds with the fast neutron of research reactors has been hampered by difficulties in accurately measuring dose and in obtaining repeatable and comparable results. Co-ordinated research under an international program organized by the FAO and IAEA has already resulted in significant improvements in methods of exposing seeds in research reactors and in obtaining accurate dosimetry. This has been accomplished by the development of a standard reactor facility for the neutron irradiation of seeds and standard methods for determining fast-neutron dose and the biological response after irradiation. In this program various divisions of the IAEA and the Joint FAO/IAEA Division co-operate with a number of research institutes and reactor centres throughout the world. Results of the preliminary experiments were reported in Technical Reports Series No. 76, ''Neutron Irradiation of Seeds''. This volume contains the proceedings of a meeting of co-operators in the FAO/IAEA Neutron Seed Irradiation Program and other active scientists in this field. The meeting was held in Vienna from 11 to 15 December 1967. Refs, figs and tabs.

  7. Neutron irradiation of seeds 2

    International Nuclear Information System (INIS)

    1968-01-01

    The irradiation of seeds with the fast neutron of research reactors has been hampered by difficulties in accurately measuring dose and in obtaining repeatable and comparable results. Co-ordinated research under an international program organized by the FAO and IAEA has already resulted in significant improvements in methods of exposing seeds in research reactors and in obtaining accurate dosimetry. This has been accomplished by the development of a standard reactor facility for the neutron irradiation of seeds and standard methods for determining fast-neutron dose and the biological response after irradiation. In this program various divisions of the IAEA and the Joint FAO/IAEA Division co-operate with a number of research institutes and reactor centres throughout the world. Results of the preliminary experiments were reported in Technical Reports Series No. 76, ''Neutron Irradiation of Seeds''. This volume contains the proceedings of a meeting of co-operators in the FAO/IAEA Neutron Seed Irradiation Program and other active scientists in this field. The meeting was held in Vienna from 11 to 15 December 1967. Refs, figs and tabs

  8. [Dynamics of seed rain of Tripterygium hypoglaucum and soil seed bank].

    Science.gov (United States)

    Zhang, Zhi-Wei; Wei, Yong-Sheng; Liu, Xiang; Su, Shu; Qu, Xian-You; Wang, Chang-Hua

    2017-11-01

    Tripterygium hypoglaucum is an endangered species in arid areas of Xiannvshan Chongqing, China. The dynamic characteristics of seed rain and soil seed bank of T. hypoglaucum were studied in this paper.Results showed that T. hypoglaucum years of mature seeds distribution number up to October; the seed rain occurred from the last ten-day of September to in the first ten-day of November and the peak of scattered seed rain concentrated in the October.The numbers of soil seed bank at 2-5 cm soil layer,mainly concentrated in the 1.5-3.5 m range. T. hypoglaucum seeds to the wind as a force for transmission, the transmission ability is strong, but in the process of natural reproduction, full mature seed rate is low, the soil seed bank seeds seed short-lived factors these were unfavorable for the natural reproduction of T. hypoglaucum population. Copyright© by the Chinese Pharmaceutical Association.

  9. Factors influencing upon the incidence of seed migration in I-125 seed transperineal prostate implantation

    International Nuclear Information System (INIS)

    Itami, Jun; Onishi, Kayoko; Kanemura, Mikio

    2005-01-01

    Transperineal I-125 seed brachytherapy for prostate cancer is rapidly expanding in Japan. Seed migrations to lung and abdomen are well known complication in the seed brachytherapy. The rate of incidence and the predisposing factors were studied. From April 2004 through January 2005, 36 patients underwent transperineal I-125 seed brachytherapy for prostate cancer. In all patients loose I-125 seeds were inserted with Mick applicator according to modified peripheral loading pattern. One day, 1 week, and 1 month after the procedure, posteroanterior and lateral chest X-rays and abdominal X-ray were performed. Abdominal and chest seed migrations were seen in 11 (30.6%) and 14 (38.9%) patients, respectively. In total, 20 patients (55.6%) showed seed migrations. Forty-two I-125 seeds migrated out of 2,508 implanted seeds. Most of the migrations were seen until 1 month after the procedure. The preplanned number of the extraprostatic seeds had a statistically significant influence upon the incidence of seed migration. Seed migration is not a rare phenomenon in transperineal I-125 seed brachytherapy for prostate cancer. To confirm seed migration, X-ray examinations 1 month after the procedure are suited. At the preplanning, the number of extraprostatic seeds should be limited to minimal to decrease the incidence of seed migration. In future, the introduction of linked I-125 seeds is preferred. (author)

  10. Ash (Fraxinus excelsior) seed quality in relation to seed ...

    African Journals Online (AJOL)

    Jane

    2011-07-18

    Jul 18, 2011 ... bottles were seal-locked and kept in the accelerated ageing cham- ber at three ... Hundred (100) seeds of each treatment were performed in plastic ..... by heat stress: the activation state of Rubisco as a limiting factor in.

  11. Effects of seed fermentation method on seed germination and vigor ...

    African Journals Online (AJOL)

    BERTIN

    2013-11-27

    Nov 27, 2013 ... high, suggesting that wet soil is necessary for an on farm reliable evaluation of seed fermentation method in the .... with tap water, and sundried in ambient air until attaining 6 to 7% moisture. ..... arabica in Brazil. Int. J. Food ...

  12. Nutrient composition of Dacryodes edulis seed and seed coat mixture

    Directory of Open Access Journals (Sweden)

    C.U. OGUNKA-NNOKA

    2017-07-01

    Full Text Available This study investigated the nutrient composition of D. edulis seed and seed coat mixture. Qualitative and quantitative phytochemicals, proximate, and vitamin compositions were evaluated using standard methods. Saponins were very high, alkaloids, flavonoids, and tannins were high, while terpenoids were low, and glycosides, aldehydes, and steroids were absent. The quantitative phytochemical determination followed the order; saponin > kaempferol > rutin > catechin > tannin > sapogenin > lunamarine > phenol > ribalinidine > anthocyanin > oxalate > phytate. For the proximate composition, carbohydrates had the highest concentration, followed by lipids and fibre, while, protein concentration was the lowest. Vitamin E (5.42 mg/100g, vitamin C (3.24 mg/100g, and vitamin A (2.84 mg/100g were the highest occurring constituent vitamins while vitamin B12 (0.035 mg/100g and vitamin B2 (0.075 mg/100g were the least occurring vitamins. This study has shown the rich phytochemical composition of D. edulis seed and seed coat mixture while showing deficiencies in proteins, distinct vitamins, and ash contents.

  13. Chlorophyll in tomato seeds: marker for seed performance?

    NARCIS (Netherlands)

    Suhartanto, M.R.

    2002-01-01

    Using Xe-PAM, laser induced fluorometry and high performance liquid chromatography we found that chlorophyll was present in young tomato (cv. Moneymaker) seeds and was degraded during maturation. Fluorescence microscopy and imaging showed that the majority of chlorophyll is located in the

  14. The role of seed priming in improving seed germination and ...

    African Journals Online (AJOL)

    win7

    2013-11-13

    Nov 13, 2013 ... As a result, fresh and dry weights of shoot and root were improved. Seed priming alleviated the inhibitory effect of salt stress on germination and seedling growth of maize under salt stress. ... Salinization is more spreading in irrigated lands because of inappropriate management of irrigation and drainage.

  15. Seed producer cooperatives in the Ethiopian seed sector and their role in seed supply improvement: A review

    NARCIS (Netherlands)

    Sisay, D.T.; Verhees, F.J.H.M.; Trijp, van J.C.M.

    2017-01-01

    The role of seed producer cooperatives (SPCs) in the Ethiopian seed sector and their contribution to seed supply improvement have received attention from researchers, policymakers, and development partners. However, limited work has been done in reviewing and documenting their involvement in the

  16. Forest Seed Collection, Processing,and Testing

    DEFF Research Database (Denmark)

    Schmidt, Lars Holger

    2016-01-01

    This chapter pertains to the techniques of capturing the best genetic quality seeds a seed source can produce at the optimal time of high physiological maturity and maintaining these qualities throughout the handling processes, all at a minimum cost. Different collection and processing techniques...... apply to different species, seed types, situations, and purposes. Yet the collection and processing toolbox contains a number of “standard” methods for most of these groups. Records and documentation help in evaluating “best practice” for future method improvement, and it helps in linking offspring...... to seed source. Conditions are set for short- and long-term seed storage by their inert storability physiology. The potential storage life of seed may for some robust “orthodox” species be several decades, while no available storage conditions can maintain viability for sensitive “recalcitrant” seed. Seed...

  17. Chemical Composition of Kapok (Ceibapentandra) Seed and ...

    African Journals Online (AJOL)

    -5 years. ... with a sharp steel knife from which healthy seeds were selected. ... Total carbohydrate was determined by difference. .... resistance to microbial attack of plant tissue (Taiz and. Zeiger, 1991 ... Maintenance of soybean seed quality in ...

  18. Inheritance of egusi seed type in watermelon.

    Science.gov (United States)

    Gusmini, G; Wehner, T C; Jarret, R L

    2004-01-01

    An unusual seed mutant in watermelon (Citrullus lanatus var. lanatus) has seeds with a fleshy pericarp, commonly called egusi seeds. The origin of the phenotype is unknown, but it is widely cultivated in Nigeria for the high protein and carbohydrate content of the edible seeds. Egusi seeds have a thick, fleshy pericarp that appears during the second to third week of fruit development. We studied the inheritance of this phenotype in crosses of normal seeded Charleston Gray and Calhoun Gray with two plant introduction accessions, PI 490383w and PI 560006, having the egusi seed type. We found that the egusi seed type is controlled by a single recessive gene, and the symbol eg was assigned. Copyright 2004 The American Genetic Association

  19. Seed technology training in the year 2000

    Directory of Open Access Journals (Sweden)

    McDonald M.B.

    1998-01-01

    Full Text Available Seed quality will remain the centerpiece of successful agricultural programs in the year 2000. As new changes occur in agriculture driven by advancements in biotechnology, seed enhancement technologies, a more diverse seed user clientele, and communication technologies, successful seed companies will require a knowledgeable and informed workforce to assure high seed quality. A new approach to seed technology training is professed that relies on the establishment of a three-institution consortium to achieve this objective. Advantages of the consortium are identified that emphasize the unique strengths of each institution, their geographic advantages representing major climactic/agricultural zones in the world, and differing approaches to seed technology training that are facilitated by increasing ease of global communication. This may be a better way to conduct seed technology training in the year 2000.

  20. Analysis of Seed Potato Systems in Ethiopia

    NARCIS (Netherlands)

    Hirpa, A.; Meuwissen, M.P.M.; Tesfaye, A.; Lommen, W.J.M.; Oude Lansink, A.G.J.M.; Tsegaye, A.; Struik, P.C.

    2010-01-01

    This study aimed to analyze the seed potato systems in Ethiopia, identify constraints and prioritize improvement options, combining desk research, rapid appraisal and formal surveys, expert elicitation, field observations and local knowledge. In Ethiopia, informal, alternative and formal seed

  1. Updated Methods for Seed Shape Analysis

    Directory of Open Access Journals (Sweden)

    Emilio Cervantes

    2016-01-01

    Full Text Available Morphological variation in seed characters includes differences in seed size and shape. Seed shape is an important trait in plant identification and classification. In addition it has agronomic importance because it reflects genetic, physiological, and ecological components and affects yield, quality, and market price. The use of digital technologies, together with development of quantification and modeling methods, allows a better description of seed shape. Image processing systems are used in the automatic determination of seed size and shape, becoming a basic tool in the study of diversity. Seed shape is determined by a variety of indexes (circularity, roundness, and J index. The comparison of the seed images to a geometrical figure (circle, cardioid, ellipse, ellipsoid, etc. provides a precise quantification of shape. The methods of shape quantification based on these models are useful for an accurate description allowing to compare between genotypes or along developmental phases as well as to establish the level of variation in different sets of seeds.

  2. Effects of seed and seedling predation by small mammals on ...

    African Journals Online (AJOL)

    Seed predation reduced seedling recruitment from seeds planted in March 1986 in mature fynbos, but ... Seed predation did not significantly reduce seedling recruitment from seed planted in July, August and ... AJOL African Journals Online.

  3. Use of linear discriminant function analysis in seed morphotype ...

    African Journals Online (AJOL)

    Use of linear discriminant function analysis in seed morphotype relationship study in 31 ... Data were collected on 100-seed weight, seed length and seed width. ... to the Mesoamerican gene pool, comprising the cultigroups Sieva-Big Lima, ...

  4. Challenges and opportunities for quality seed potato availability and ...

    African Journals Online (AJOL)

    ACSS

    Key words: Quality seed potato, seed system and challenges ... systems, inadequate seed production and ... informal sources of seed potato, despite ..... Directorate General for International. Co-operation (DGIC), Brussels,. Belgium. 1540pp.

  5. Efficient chaining of seeds in ordered trees

    OpenAIRE

    Allali, Julien; Chauve, Cédric; Ferraro, Pascal; Gaillard, Anne-Laure

    2010-01-01

    International audience; We consider here the problem of chaining seeds in ordered trees. Seeds are mappings between two trees Q and T and a chain is a subset of non overlapping seeds that is consistent with respect to postfix order and ancestrality. This problem is a natural extension of a similar problem for sequences, and has applications in computational biology, such as mining a database of RNA secondary structures. For the chaining problem with a set of m constant size seeds, we describe...

  6. Composition of jojoba seeds and foliage

    Energy Technology Data Exchange (ETDEWEB)

    Verbiscar, A.J.; Banigan, T.F.

    1978-01-01

    The desert shrub jojoba (Simmondsia chinensis) may be browsed by cattle. The seeds have about 50% oil but the extracted meal is at present unsuitable for feeding. Simmondsin, the most prevalent toxin, is present in seed, 2.3%, and in husks, leaves and twigs. Seeds contained another toxin, Simmondsin 2'-ferulate. The contents of oil, protein, carbohydrate and amino acids in seed are tabulated. 13 references.

  7. Drug Plant Seed Viability Preservation by Cryoconservation

    Directory of Open Access Journals (Sweden)

    Alexandra Sh. Dodonova

    2013-01-01

    Full Text Available The article considers the possibility of cryopreservation of seeds of several species of drug plants in Central Kazakhstan – Tanacetum ulutavicum, Niedzwedzkia semiretschenskia, Rhaponticum carthamoides. To increase the amount of viable seeds after liquid nitrogen freezing, we used different defrosting temperatures, deposited seeds with different moisture contents and used different containers for cryopreservation. Recommendations, concerning conditions of cryopreservation of seeds of these drug plant species were developed, basing on the obtained results.

  8. Training and research in seed technology. No quality seeds without skilled staff

    NARCIS (Netherlands)

    Groot, S.P.C.; Hilhorst, H.W.M.

    2016-01-01

    The seed industry in the Netherlands is the largest exporter of horticultural seeds, reaching farmers in every country of the world. High seed quality is one of the key factors of this success. Maintaining and increasing the level of seed qualtiy requires skilled staff, innovation and collaboration

  9. How seed orchard culture affects seed quality: experience with the southern pines

    Science.gov (United States)

    James P. Barnett

    1996-01-01

    Tree improvement programs have influenced significantly the quality of southern pine seeds produced when compared to collections from native stands. Seed orchard management practices such as fertilization can increase seed size and reduce seed dormancy. These result in the need for less complex pregermination treatments. Repeated cone collections from the same clones...

  10. Consistent individual differences in seed disperser quality in a seed-eating fish

    NARCIS (Netherlands)

    Pollux, Bart J.A.

    2017-01-01

    Animal-mediated seed dispersal (zoochory) is considered to be an important mechanism regulating biological processes at larger spatial scales. To date, intra-specific variation in seed disperser quality within seed-dispersing animals has not been studied. Here, I employed seed feeding trials to

  11. Securing Access to Seed: Social Relations and Sorghum Seed Exchange in Eastern Ethiopia

    NARCIS (Netherlands)

    McGuire, S.

    2008-01-01

    Access to seed is crucial for farming, though few studies investigate household-level access in the informal `farmer seed systems¿ which still supply most seed in poor countries. This paper uses empirical data of seed exchange practices for sorghum in eastern Ethiopia to analyze how social

  12. seed germination and seedlings growth

    African Journals Online (AJOL)

    STORAGESEVER

    2007-12-17

    Dec 17, 2007 ... however, abscisic acid (ABA) can counter this effect by keeping seeds ... Tris-HCl buffer at pH 8.8, with constant current set at 20 mA/gel. The gels were ..... on plants is rarely studied and the importance of 20E in the life cycle ...

  13. The Seed Is the Law

    Science.gov (United States)

    Antone, Eileen M.

    2005-01-01

    Since humanities arise from a specific place and from the people of that place, this article will focus on Peacemaker's revolutionary teachings about the seed of law. Long before the people from across the ocean arrived here on Turtle Island (North America) there was much warfare happening. According to John Mohawk (2001, para. 1), an Iroquoian…

  14. Seed production for fuel oils

    International Nuclear Information System (INIS)

    Mosca, G.

    1992-01-01

    With the aim of assessing commercialization prospects for vegetable oils to be used as diesel fuel alternatives, this paper provides maps indicating regional production quantities for soybean, rape and sunflower seeds in Italy. It then tables and discusses the results of energy input-output analyses carried out for rape and soybean oil production

  15. Hormones and tomato seed germination

    NARCIS (Netherlands)

    Liu, Y.

    1996-01-01

    Using GA- and ABA-deficient mutants, exogenous gibberellins (GAs), abscisic acid (ABA) and osmoticum, we studied the roles of GAs and ABA in the induction of cell cycle activities, internal free space formation and changes in water relations during seed development and imbibition in tomato. First of

  16. Spiny hopsage fruit and seed morphology

    Science.gov (United States)

    Nancy L. Shaw; Emerenciana G. Hurd; Marshall R. Haferkamp

    1996-01-01

    Rangeland seedings of spiny hopsage (Gruyia spinosa [Hook.] Moq.) may be made with either bracted utricles or seeds. Problems have resulted from inconsistent use of terminology describing these 2 structures and the fact their germination and seedling emergence is not the same with similar environmental conditions and seeding techniques. We examined...

  17. Galactinol as marker for seed longevity

    NARCIS (Netherlands)

    Souza Vidigal, De D.; Willems, L.A.J.; Arkel, van J.; Dekkers, S.J.W.; Hilhorst, H.W.M.; Bentsink, L.

    2016-01-01

    Reduced seed longevity or storability is a major problem in seed storage and contributes to increasedcosts in crop production. Here we investigated whether seed galactinol contents could be predictive forseed storability behavior in Arabidopsis, cabbage and tomato. The analyses revealed a positive

  18. Seed dormancy and germination : light and nitrate

    NARCIS (Netherlands)

    Hilhorst, H.W.M.

    1990-01-01

    One of the most important aspects of the life cycle of seed plants is the formation and development of seeds on the motherplant and the subsequent dispersal. An equally important element of the survival strategy is the ability of seeds to prevent germination in unfavorable

  19. Dormancy cycling in seeds: mechanisms and regulation

    NARCIS (Netherlands)

    Claessens, S.M.C.

    2012-01-01

    The life cycle of most plants starts, and ends, at the seed stage. In most species mature seeds are shed and dispersed on the ground. At this stage of its life cycle the seed may be dormant and will, by definition, not germinate under favourable conditions (Bewley, 1997).

    Seasonal

  20. Seed coat darkening in Cowpea bean

    Science.gov (United States)

    Seed coat of cowpea bean (Vigna unguiculata L. Walp) slowly browns to a darker color during storage. High temperature and humidity during storage might contribute to this color change. Variation in browning rate among seeds in a lot leads to a mixture of seed colors creating an unacceptable product...

  1. 7 CFR 947.12 - Seed potatoes.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Seed potatoes. 947.12 Section 947.12 Agriculture... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE IRISH POTATOES GROWN IN MODOC AND... Definitions § 947.12 Seed potatoes. Seed potatoes means and includes all potatoes officially certified and...

  2. 7 CFR 946.12 - Seed potatoes.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Seed potatoes. 946.12 Section 946.12 Agriculture... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE IRISH POTATOES GROWN IN WASHINGTON Order Regulating Handling Definitions § 946.12 Seed potatoes. Seed potatoes means and includes all...

  3. Running title: Water distribution in chickpea seeds

    African Journals Online (AJOL)

    agriphy20

    2012-07-24

    Jul 24, 2012 ... molecular mobility of cellular water in magnetically exposed seeds as compared to unexposed seeds. Analysis of ... protrusion takes place through the seed coat and absorption .... directly related to water activity (aw) of the cell water. (Gambhir ..... plants, including photosynthesis, respiration and enzymatic ...

  4. Strategies for Seed Propagation of Native Forbs

    Science.gov (United States)

    Susan E. Meyer

    2006-01-01

    Native forbs are an increasingly important component of container production for many public and private nurseries. Propagators are often called upon to grow species with unknown requirements. A systematic approach is required to obtain plants from seeds of these species, beginning with determining what is a propagule and evaluating seed quality. Next, seed dormancy...

  5. Inheritance of fresh seed dormancy in groundnut

    African Journals Online (AJOL)

    SERVER

    2008-02-19

    Feb 19, 2008 ... Seed dormancy has been defined as the failure of an intact, viable seed to ... expected ratio of 3 dormant : 1 non-dormant seeds in F2 generations of crosses of ICGV 87378 and ICGV ... ICGV 87378 x ICGV 86158. 163. 0. 163.

  6. Spinach seed quality - potential for combining seed size grading and chlorophyll flourescence sorting

    DEFF Research Database (Denmark)

    Deleuran, Lise Christina; Olesen, Merete Halkjær; Boelt, Birte

    2013-01-01

    might therefore improve the establishment of spinach for producers. Spinach seeds were harvested at five different times (H1, H2, H3, H4 and H5) starting 3 weeks before estimated optimum harvest time. The harvested seeds were sorted according to chlorophyll fluorescence (CF) and seed size. Two harvest.......5–3.25 mm size seeds had germinated on day 3 than both their larger and smaller counterparts at the later time of harvest (H4). Seeds with a diameter below 2.5 mm displayed the lowest MGT. Commercially, the use of chlorophyll fluorescence (CF)-sorted seeds, in combination with seed size sorting, may provide...

  7. [Effects of illumination and seed-soaking reagent on seed germination of Solanum nigrum].

    Science.gov (United States)

    Yang, Chuan-Jie; Wei, Shu-He; Zhou, Qi-Xing; Hu, Ya-Hu; Niu, Rong-Cheng

    2009-05-01

    To explore a rapid seed germination method for hyperaccumulator Solanum nigrum, a germination experiment with different illumination and seed-soaking treatments was conducted in constant temperature box and greenhouse, with filter as burgeon base. Under illumination, the germination rate was about 5 times high of that without illumination (P seed germination of S. nigrum. All test seed-soaking reagents could significantly improve the germination rate of S. nigrum (P seeds treated with H2O2 had the shortest germination time. The germination rate of seeds soaked but without cleaning was 2-3 times as high as that of seeds soaked and cleaned with water.

  8. The biomechanics of seed germination.

    Science.gov (United States)

    Steinbrecher, Tina; Leubner-Metzger, Gerhard

    2017-02-01

    From a biomechanical perspective, the completion of seed (and fruit) germination depends on the balance of two opposing forces: the growth potential of the embryonic axis (radicle-hypocotyl growth zone) and the restraint of the seed-covering layers (endosperm, testa, and pericarp). The diverse seed tissues are composite materials which differ in their dynamic properties based on their distinct cell wall composition and water uptake capacities. The biomechanics of embryo cell growth during seed germination depend on irreversible cell wall loosening followed by water uptake due to the decreasing turgor, and this leads to embryo elongation and eventually radicle emergence. Endosperm weakening as a prerequisite for radicle emergence is a widespread phenomenon among angiosperms. Research into the biochemistry and biomechanics of endosperm weakening has demonstrated that the reduction in puncture force of a seed's micropylar endosperm is environmentally and hormonally regulated and involves tissue-specific expression of cell wall remodelling proteins such as expansins, diverse hydrolases, and the production of directly acting apoplastic reactive oxygen. The endosperm-weakening biomechanics and its underlying cell wall biochemistry differ between the micropylar (ME) and chalazal (CE) endosperm domains. In the ME, they involve cell wall loosening, cell separation, and programmed cell death to provide decreased and localized ME tissue resistance, autolysis, and finally the formation of an ME hole required for radicle emergence. Future work will further unravel the molecular mechanisms, environmental regulation, and evolution of the diverse biomechanical cell wall changes underpinning the control of germination by endosperm weakening. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  9. Improving seed potato quality in Ethiopia: a value chain perspective

    NARCIS (Netherlands)

    Hirpa, A.; Gielen-Meuwissen, M.P.M.; Lommen, W.J.M.; Oude Lansink, A.G.J.M.; Tsegaye, A.; Struik, Paul

    2016-01-01

    In Ethiopia, use of low-quality seed potatoes by the majority of potato growers is
    associated with underdevelopment of the seed potato value chains. Three seed potato systems are present in Ethiopia: the informal seed system, the alternative seed system and the formal seed system. This chapter

  10. Evaluation of seed production of scots pine ( Pinus sylvestris L ...

    African Journals Online (AJOL)

    This research was carried out to investigate seed production in a 13 years-old scots pine (Pinus sylvestris L.) clonal seed orchard, including 30 clones. Eight of cone and seed traits as number of fertile and infertile scales, cone volume, cone number, filled and empty seed number, seed efficiency and 1000 seed weight were ...

  11. Multivariate NIR studies of seed-water interaction in Scots Pine Seeds (Pinus sylvestris L.)

    OpenAIRE

    Lestander, Torbjörn

    2003-01-01

    This thesis describes seed-water interaction using near infrared (NIR) spectroscopy, multivariate regression models and Scots pine seeds. The presented research covers classification of seed viability, prediction of seed moisture content, selection of NIR wavelengths and interpretation of seed-water interaction modelled and analysed by principal component analysis, ordinary least squares (OLS), partial least squares (PLS), bi-orthogonal least squares (BPLS) and genetic algorithms. The potenti...

  12. Influence the Rubber Seed Type and Altitude on Characteristic of Seed, Oil and Biodiesel

    OpenAIRE

    Salni Salni; Poedji Loekitowati Hariani; Hanifa Marisa Hanifa

    2017-01-01

    This research studies the influence of the type of rubber seed that is superior and local, altitude plant in South Sumatra province to the characteristic of seed, oil and biodiesel (methyl ester). Rubber plants planted from local rubber seed by seeds seedlings and superior rubber seed by selected clones. In the study, rubber plants planted at a different altitude, namely in Banyuasin district (18 m above sea level), Prabumulih District (176 m above sea level) and Lahat District (627 m above s...

  13. Influence of the Rubber Seed Type and Altitude on Characteristic of Seed, Oil and Biodiesel

    OpenAIRE

    Salni, Salni; Hariani, Poedji Loekitowati; Hanifa, Hanifa Marisa

    2017-01-01

    This research studies the influence of the type of rubber seed that is superior and local, altitude plant in South Sumatra province to the characteristic of seed, oil and biodiesel (methyl ester). Rubber plants planted from local rubber seed by seeds seedlings and superior rubber seed by selected clones. In the study, rubber plants planted at a different altitude, namely in Banyuasin district (18 m above sea level), Prabumulih District (176 m above sea level) and Lahat District (627 m above s...

  14. [Procedure of seed quality testing and seed grading standard of Prunus humilis].

    Science.gov (United States)

    Wen, Hao; Ren, Guang-Xi; Gao, Ya; Luo, Jun; Liu, Chun-Sheng; Li, Wei-Dong

    2014-11-01

    So far there exists no corresponding quality test procedures and grading standards for the seed of Prunus humilis, which is one of the important source of base of semen pruni. Therefor we set up test procedures that are adapt to characteristics of the P. humilis seed through the study of the test of sampling, seed purity, thousand-grain weight, seed moisture, seed viability and germination percentage. 50 cases of seed specimens of P. humilis tested. The related data were analyzed by cluster analysis. Through this research, the seed quality test procedure was developed, and the seed quality grading standard was formulated. The seed quality of each grade should meet the following requirements: for first grade seeds, germination percentage ≥ 68%, thousand-grain weight 383 g, purity ≥ 93%, seed moisture ≤ 5%; for second grade seeds, germination percentage ≥ 26%, thousand-grain weight ≥ 266 g, purity ≥ 73%, seed moisture ≤9%; for third grade seeds, germination percentage ≥ 10%, purity ≥ 50%, thousand-grain weight ≥ 08 g, seed moisture ≤ 13%.

  15. Myrmecochory and short-term seed fate in Rhamnus alaternus: Ant species and seed characteristics

    Science.gov (United States)

    Bas, J. M.; Oliveras, J.; Gómez, C.

    2009-05-01

    Benefits conferred on plants in ant-mediated seed dispersal mutualisms (myrmecochory) depend on the fate of transported seeds. We studied the effects of elaiosome presence, seed size and seed treatment (with and without passage through a bird's digestive tract) on short-term seed fate in Rhamnus alaternus. In our study, we define short-term seed, or initial, seed fate, as the location where ants release the seeds after ant contact with it. The elaiosomes had the most influence on short-term fate, i.e. whether or not seeds were transported to the nest. The workers usually transported big seeds more often than small ones, but small ants did not transport large seeds. Effect of seed size on transport depended on the ant species and on the treatment of the seed (manual extraction simulating a direct fall from the parent plant vs. bird deposition corresponding to preliminary primary dispersal). Probability of removal of elaiosome-bearing seeds to the nest by Aphaenogaster senilis increased with increasing seed weight.

  16. Trade and Transfer of Tree Seed

    DEFF Research Database (Denmark)

    Schmidt, Lars Holger

    2016-01-01

    testing records. Genetic quality is documented as documents on origin or seed source. New types of tree planting by smallholders imply special problems in distribution and supply systems since production systems for tree seeds have large areas while many consumers have small space for planting....... A centralized forest seed supply contains large central units with good facilities for production and procurement but is far from seed users. Alternative decentralized systems with many small producers may have problems meeting high standards of seed quality and dealing with central regulations....

  17. Stimulation of lettuce seed germination by ethylene.

    Science.gov (United States)

    Abeles, F B; Lonski, J

    1969-02-01

    Ethylene increased the germination of freshly imbibed lettuce (Lactuca sativa L. var. Grand Rapids) seeds. Seeds receiving either red or far-red light or darkness all showed a positive response to the gas. However, ethylene was apparently without effect on dormant seeds, those which failed to germinate after an initial red or far-red treatment. Carbon dioxide, which often acts as a competitive inhibitor of ethylene, failed to clearly reverse ethylene-enhanced seed germination. While light doubled ethylene production from the lettuce seeds, its effect was not mediated by the phytochrome system since both red and far-red light had a similar effect.

  18. GERMINATION STUDIES ON Tabebuia impetiginosa Mart. SEEDS

    Directory of Open Access Journals (Sweden)

    Edvaldo Aparecido Amaral da Silva

    2004-06-01

    Full Text Available Seed germination and seedling production of native forest tree species are an important step in ex situ conservation programs and in the reforestation with ecological purposes. Therefore, understanding seed germination and its regulation is mandatory for the complete success of the conservation programs and revegetation techniques. Thus, morphological studies, temperature requirements for seed germination and its control by gibberellins (GAs were studied in Tabebuia impetiginosa (“ipê-roxo” seeds. The best temperature for germination under constant light was 30oC. The imbibition of T. impetiginosa seeds followed the common triphasic pattern, with most of the seeds attaining phase II at 24 hours and phase III at 72 hours of imbibition. Visible germination, as radicle elongation, started at 30 hours in water-imbibed seeds and at 24 hours in GA-imbibed seeds. Seeds imbibed in Paclobutrazol, an inhibitor of GA biosynthesis, failed to germinate. However, application of exogenous gibberellins overcame inhibition and allowed germination, suggesting that GAs are regulators of Tabebuia impetiginosa seed germination. The results suggested that germination in Tabebuia impetiginosa seeds is controlled by elongation of the radicle and gibberellins may play an important role in regulating it. The possible role of gibberellins is discussed.

  19. [Seed quality test methods of Paeonia suffruticosa].

    Science.gov (United States)

    Cao, Ya-Yue; Zhu, Zai-Biao; Guo, Qiao-Sheng; Liu, Li; Wang, Chang-Lin

    2014-11-01

    In order to optimize the testing methods for Paeonia suffruticosa seed quality, and provide basis for establishing seed testing rules and seed quality standard of P. suffruticosa. The seed quality of P. suffruticosa from different producing areas was measured based on the related seed testing regulations. The seed testing methods for quality items of P. suffruticosa was established preliminarily. The samples weight of P. suffruticosa was at least 7 000 g for purity analysis and was at least 700 g for test. The phenotypic observation and size measurement were used for authenticity testing. The 1 000-seed weight was determined by 100-seed method, and the water content was carried out by low temperature drying method (10 hours). After soaking in distilled water for 24 h, the seeds was treated with different temperature stratifications of day and night (25 degrees C/20 degrees C, day/night) in the dark for 60 d. After soaking in the liquor of GA3 300 mg x L(-1) for 24 h, the P. suffruticos seeds were cultured in wet sand at 15 degrees C for 12-60 days for germination testing. Seed viability was tested by TlC method.

  20. Is seed conditioning essential for Orobanche germination?

    Science.gov (United States)

    Plakhine, Dina; Ziadna, Hammam; Joel, Daniel M

    2009-05-01

    Parasitic Orobanchaceae germinate only after receiving a chemical stimulus from roots of potential host plants. A preparatory phase of several days that follows seed imbibition, termed conditioning, is known to be required; thereafter the seeds can respond to germination stimulants. The aim of this study was to examine whether conditioning is essential for stimulant receptivity. Non-conditioned seeds of both Orobanche cumana Wallr. and O. aegyptiaca Pers. [syn. Phelipanche aegyptiaca (Pers.) Pomel] were able to germinate in response to chemical stimulation by GR24 even without prior conditioning. Stimulated seeds reached maximal germination rates about 2 weeks after the onset of imbibition, no matter whether the seeds had or had not been conditioned before stimulation. Whereas the lag time between stimulation and germination response of non-conditioned seeds was longer than for conditioned seeds, the total time between imbibition and germination was shorter for the non-conditioned seeds. Unlike the above two species, O. crenata Forsk. was found to require conditioning prior to stimulation. Seeds of O. cumana and O. aegyptiaca are already receptive before conditioning. Thus, conditioning is not involved in stimulant receptivity. A hypothesis is put forward, suggesting that conditioning includes (a) a parasite-specific early phase that allows the imbibed seeds to overcome the stress caused by failing to receive an immediate germination stimulus, and (b) a non-specific later phase that is identical to the pregermination phase between seed imbibition and actual germination that is typical for all higher plants.

  1. Seed counting system evaluation using arduino microcontroller

    Directory of Open Access Journals (Sweden)

    Paulo Fernando Escobar Paim

    2018-01-01

    Full Text Available The development of automated systems has been highlighted in the most diverse productive sectors, among them, the agricultural sector. These systems aim to optimize activities by increasing operational efficiency and quality of work. In this sense, the present work has the objective of evaluating a prototype developed for seed count in laboratory, using Arduino microcontroller. The prototype of the system for seed counting was built using a dosing mechanism commonly used in seeders, electric motor, Arduino Uno, light dependent resistor and light emitting diode. To test the prototype, a completely randomized design (CRD was used in a two-factorial scheme composed of three groups defined according to the number of seeds (500, 1000 and 1500 seeds tested, three speeds of the dosing disc that allowed the distribution in 17, 21 and 32 seeds per second, with 40 repetitions evaluating the seed counting prototype performance in different speeds. The prototype of the bench counter showed a moderate variability of seed number of counted within the nine tests and a high precision in the seed count on the distribution speeds of 17 and 21 seeds per second (s-1 up to 1500 seeds tested. Therefore, based on the observed results, the developed prototype presents itself as an excellent tool for counting seeds in laboratory.

  2. Interactions between seed traits and digestive processes determine the germinability of bird-dispersed seeds.

    Science.gov (United States)

    Kleyheeg, Erik; Claessens, Mascha; Soons, Merel B

    2018-01-01

    Waterbirds disperse a wide range of plant seeds via their guts, promoting biotic connectivity between isolated habitat patches. However, the intensity of digestive forces encountered by seeds, and therefore their potential to survive digestive tract passage, varies within and between waterbird species. Here, we investigate under controlled conditions how the interaction between seed traits and digestive strategies affect the germinability of seeds following waterbird-mediated dispersal. We exposed seeds of 30 wetland plant species to the main digestive processes in the dabbling duck digestive system: mechanical, chemical and intestinal digestion. These were simulated by 1) a pressure test and scarification treatment, 2) incubation in simulated gastric juice, and 3) incubation in intestinal contents of culled mallards (Anas platyrhynchos). We evaluated their separate and combined effects on seed germination, and identified the role of seed size and seed coat traits in resisting the digestive forces. Seeds were generally resistant to separate digestive processes, but highly sensitive to a combination. Resistance to mechanical break-down was reduced by up to 80% by chemical pre-treatment, especially for seeds with permeable coats. Scarified seeds were 12-17% more vulnerable to chemical and intestinal digestive processes than undamaged seeds. Large seeds and seeds with thin, permeable coats were particularly sensitive to chemical and intestinal digestion. These results indicate that efficient digestion of seeds requires multiple digestive processes. The gizzard, responsible for mechanical digestion, plays a key role in seed survival. Omnivorous birds, which have relatively light gizzards compared to pure herbivores or granivores, are thus most likely to disperse seeds successfully. Regardless of digestive strategy, small seeds with tough seed coats are most resistant to digestion and may be adapted to endozoochorous dispersal by waterbirds.

  3. Interactions between seed traits and digestive processes determine the germinability of bird-dispersed seeds

    Science.gov (United States)

    Soons, Merel B.

    2018-01-01

    Waterbirds disperse a wide range of plant seeds via their guts, promoting biotic connectivity between isolated habitat patches. However, the intensity of digestive forces encountered by seeds, and therefore their potential to survive digestive tract passage, varies within and between waterbird species. Here, we investigate under controlled conditions how the interaction between seed traits and digestive strategies affect the germinability of seeds following waterbird-mediated dispersal. We exposed seeds of 30 wetland plant species to the main digestive processes in the dabbling duck digestive system: mechanical, chemical and intestinal digestion. These were simulated by 1) a pressure test and scarification treatment, 2) incubation in simulated gastric juice, and 3) incubation in intestinal contents of culled mallards (Anas platyrhynchos). We evaluated their separate and combined effects on seed germination, and identified the role of seed size and seed coat traits in resisting the digestive forces. Seeds were generally resistant to separate digestive processes, but highly sensitive to a combination. Resistance to mechanical break-down was reduced by up to 80% by chemical pre-treatment, especially for seeds with permeable coats. Scarified seeds were 12–17% more vulnerable to chemical and intestinal digestive processes than undamaged seeds. Large seeds and seeds with thin, permeable coats were particularly sensitive to chemical and intestinal digestion. These results indicate that efficient digestion of seeds requires multiple digestive processes. The gizzard, responsible for mechanical digestion, plays a key role in seed survival. Omnivorous birds, which have relatively light gizzards compared to pure herbivores or granivores, are thus most likely to disperse seeds successfully. Regardless of digestive strategy, small seeds with tough seed coats are most resistant to digestion and may be adapted to endozoochorous dispersal by waterbirds. PMID:29614085

  4. Evaluation of Wheat (Triticum aestivum, L. Seed Quality of Certified Seed and Farm-Saved Seed in Three Provinces of Iran

    Directory of Open Access Journals (Sweden)

    Khazaei Fardin

    2016-06-01

    Full Text Available The objective of this study was to study the seed quality aspects of wheat (Triticum aestivum L. and the extent of weed seed contamination present in wheat seeds produced in different regions of Iran. Four districts (cities, each including 12 fields (six certified seed fields and six farm-saved seed fields, were selected in each regions (provinces. One kilogram of the wheat seed sample was collected from each field for analysis in the laboratory. Wheat seeding was commonly done by farm-saved seed sourced from within the farm due to the high costs of certified seeds purchased from outside sources, followed by the low seed quality. The use of a farm-saved seed resulted in a higher germination rate and a lower mean time to germination compared with another system. The more positive temperatures experienced by mother plants could decrease the number of normal seedling and seedling length vigor index. Generally there was virtually no difference about physiological quality between certified seed and farm-saved seed sector that is related to lower quality of certified seed. The certified produced seeds had the lower number of weed seed, species and genus before and after cleaning. The highest seed purity and 1000 seed weight was obtained from the certified seed production system. The need for cleaning the farm-saved seed samples before sowing is one of the important findings of this survey.

  5. Effects of elevated CO2 and temperature on seed quality

    DEFF Research Database (Denmark)

    Hampton, John G; Boelt, Birte; Rolston, M P

    2013-01-01

    production on three seed quality components: seed mass, germination and seed vigour. In response to elevated CO2, seed mass has been reported to both increase and decrease in C3 plants, but not change in C4 plants. Increases are greater in legumes than non-legumes, and there is considerable variation among...... species. Seed mass increases may result in a decrease of seed nitrogen (N) concentration in non-legumes. Increasing temperature may decrease seed mass because of an accelerated growth rate and reduced seed filling duration, but lower seed mass does not necessarily reduce seed germination or vigour. Like...

  6. Noahs Ark of the seeds

    International Nuclear Information System (INIS)

    Tristan, R. M.

    2015-01-01

    The so-called 'vault of the end of the world', located in the archipelago Arctic Svalbard, brings together more than 860,000 samples seeds from around the world. The purpose of your existence -like the of thousands of other banks of its kind in all the planet - is to preserve the genetic diversity of crops, most of them are now disappearing. An international treaty approved by the United Nations, and in force since 2004, allowed the construction of this 'Sanctuary' where more than one hundred countries maintain their own banks of seeds, freezing them to 18 degrees below zero to lower its biological activity. (Author)

  7. Increasing seed size and quality by manipulating BIG SEEDS1 in legume species.

    Science.gov (United States)

    Ge, Liangfa; Yu, Jianbin; Wang, Hongliang; Luth, Diane; Bai, Guihua; Wang, Kan; Chen, Rujin

    2016-11-01

    Plant organs, such as seeds, are primary sources of food for both humans and animals. Seed size is one of the major agronomic traits that have been selected in crop plants during their domestication. Legume seeds are a major source of dietary proteins and oils. Here, we report a conserved role for the BIG SEEDS1 (BS1) gene in the control of seed size and weight in the model legume Medicago truncatula and the grain legume soybean (Glycine max). BS1 encodes a plant-specific transcription regulator and plays a key role in the control of the size of plant organs, including seeds, seed pods, and leaves, through a regulatory module that targets primary cell proliferation. Importantly, down-regulation of BS1 orthologs in soybean by an artificial microRNA significantly increased soybean seed size, weight, and amino acid content. Our results provide a strategy for the increase in yield and seed quality in legumes.

  8. Mycoflora in Exhumed Seeds of Opuntia tomentosa and Its Possible Role in Seed Germination

    Directory of Open Access Journals (Sweden)

    María Esther Sánchez-Coronado

    2011-01-01

    Full Text Available The funicular cover of the Opuntia tomentosa seed limits imbibition; germination occurs only when the funicle is weakened or the funicular valve is removed. We investigated the role of fungi in funicular weakening and seed germination. Seeds that had been either buried in one of two sites or stored in the laboratory were germinated with and without a valve. Disinfected or nondisinfected seeds and their naked embryos were cultivated on agar or PDA. None of the 11 identified fungal genera grew on the disinfected control seeds or the embryos. The mycoflora present on disinfected and nondisinfected exhumed seeds suggest that the fungal colonization occurred in the soil and differed between the burial sites. Exhumed seeds with and without a valve germinated in high percentages, whereas only the control seeds without a valve germinated. Scanning electron micrographs showed that the hyphae penetrated, cracked, and eroded the funicular envelope of exhumed seeds.

  9. Alleviate Seed Ageing Effects in Silybum marianum by Application of Hormone Seed Priming

    Directory of Open Access Journals (Sweden)

    Seyed Ata SIADAT

    2015-09-01

    Full Text Available Most of the medicinal plants have seed dormancy or poor seed germination. This is due to their natural compounds or their morphological structure. Existence of such mechanisms makes the seeds able to endure harsh environments, stressful conditions or natural ageing. Different seed enhancement treatments were proposed in order to improve seed germination. In this study, it was examined Silybum marianum seed longevity (0, 48 and 72 hour of ageing and the response to seed priming when using different concentrations of cytokinin (50, 200, 350 and 500 ppm and different duration of treatment (8, 12 and 24 hour. Results revealed that ageing could be successfully alleviated using seed priming. Seed ageing significantly affected seed germination and vigour. Application of cytokinin considerably improved seed vigour in aged and non aged seeds. The most effective seed priming treatment was with 500 ppm cytokine, with the duration of 24 hours. In conclusion, it can be recommend the used of plant growth regulators like cytokine, as a good priming agent, to recover losses of seed quality and improve germination characteristics.

  10. Agrowaste: Phytosterol from Durian Seed

    OpenAIRE

    D. Mohd Nazrul Hisham; J. Mohd Lip; R. Suri; H. Mohamed Shafit; Z.Kharis; K. Shazlin; A. Normah; M.F. Nurul Nabilah

    2012-01-01

    Presence of phytosterol compound in Durian seed (Durio zibethinus) or known as King of fruits has been discovered from screening work using reagent test. Further analysis work has been carried out using mass spectrometer in order to support the priliminary finding. Isolation and purification of the major phytosterol has been carried out using an open column chromatography. The separation was monitored using thin layer chromatography (TLC). Major isolated compounds and pur...

  11. Preplanting irradiation of tomato seeds

    International Nuclear Information System (INIS)

    Maltseva, S.

    1976-01-01

    Seeds of the tomato varieties Pioneer-2, Drouzhba and Ace were treated prior to planting with Co 60 gamma rays in optimal doses of 2000 R and the varieties No 10 x Bison, Triumph and Extase with 1500 R. This treatment raised the germination energy and the plants started flowering and ripening earlier. The index of earliness was enhanced but the overall yield was equal to that of the control plants. (author)

  12. Proteomics of Rice Seed Germination

    Directory of Open Access Journals (Sweden)

    Dongli eHe

    2013-07-01

    Full Text Available Seed is a condensed form of plant. Under suitable environmental conditions, it can resume the metabolic activity from physiological quiescent status, and mobilize the reserves, biosynthesize new proteins, regenerate organelles and cell membrane, eventually protrude the radicle and enter into seedling establishment. So far, how these activities are regulated in a coordinated and sequential manner is largely unknown. With the availability of more and more genome sequence information and the development of mass spectrometry (MS technology, proteomics has been widely applied in analyzing the mechanisms of different biological processes, and proved to be very powerful. Regulation of rice seed germination is critical for rice cultivation. In recent years, a lot of proteomic studies have been conducted in exploring the gene expression regulation, reserves mobilization and metabolisms reactivation, which brings us new insights on the mechanisms of metabolism regulation during this process. Nevertheless, it also invokes a lot of questions. In this mini-review, we summarized the progress in the proteomic studies of rice seed germination. The current challenges and future perspectives were also discussed, which might be helpful for the following studies.

  13. PHO1 Exports Phosphate from the Chalazal Seed Coat to the Embryo in Developing Arabidopsis Seeds.

    Science.gov (United States)

    Vogiatzaki, Evangelia; Baroux, Célia; Jung, Ji-Yul; Poirier, Yves

    2017-10-09

    Seed production requires the transfer of nutrients from the maternal seed coat to the filial endosperm and embryo. Because seed coat and filial tissues are symplasmically isolated, nutrients arriving in the seed coat via the phloem must be exported to the apoplast before reaching the embryo. Proteins implicated in the transfer of inorganic phosphate (Pi) from the seed coat to the embryo are unknown despite seed P content being an important agronomic trait. Here we show that the Arabidopsis Pi exporters PHO1 and PHOH1 are expressed in the chalazal seed coat (CZSC) of developing seeds. PHO1 is additionally expressed in developing ovules. Phosphorus (P) content and Pi flux between the seed coat and embryo were analyzed in seeds from grafts between WT roots and scions from either pho1, phoh1, or the pho1 phoh1 double mutant. Whereas P content and distribution between the seed coat and embryo in fully mature dry seeds of these mutants are similar to the WT, at the mature green stage of seed development the seed coat of the pho1 and pho1 phoh1 mutants, but not of the phoh1 mutant, retains approximately 2-fold more P than its WT control. Expression of PHO1 under a CZSC-specific promoter complemented the seed P distribution phenotype of the pho1 phoh1 double mutant. CZSC-specific down-expression of PHO1 also recapitulated the seed P distribution phenotype of pho1. Together, these experiments show that PHO1 expression in the CZSC is important for the transfer of P from the seed coat to the embryo in developing seeds. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Radioactive seed immobilization techniques for interstitial brachytherapy

    International Nuclear Information System (INIS)

    Yan, K.; Podder, T.; Buzurovic, I.; Hu, Y.; Dicker, A.; Valicenti, R.; Yu, Y.; Messing, E.; Rubens, D.; Sarkar, N.; Ng, W.

    2008-01-01

    In prostate brachytherapy, seeds can detach from their deposited sites and move locally in the pelvis or migrate to distant sites including the pulmonary and cardiac regions. Undesirable consequences of seed migration include inadequate dose coverage of the prostate and tissue irradiation effects at the site of migration. Thus, it is clinically important to develop seed immobilization techniques. We first analyze the possible causes for seed movement, and propose three potential techniques for seed immobilization: (1) surgical glue, (2) laser coagulation and (3) diathermy coagulation. The feasibility of each method is explored. Experiments were carried out using fresh bovine livers to investigate the efficacy of seed immobilization using surgical glue. Results have shown that the surgical glue can effectively immobilize the seeds. Evaluation of the radiation dose distribution revealed that the non-immobilized seed movement would change the planned isodose distribution considerably; while by using surgical glue method to immobilize the seeds, the changes were negligible. Prostate brachytherapy seed immobilization is necessary and three alternative mechanisms are promising for addressing this issue. Experiments for exploring the efficacy of the other two proposed methods are ongoing. Devices compatible with the brachytherapy procedure will be designed in future. (orig.)

  15. Partial dehydration and cryopreservation of Citrus seeds.

    Science.gov (United States)

    Graiver, Natalia; Califano, Alicia; Zaritzky, Noemí

    2011-11-01

    Three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. Intermediate seeds cannot be stored in liquid nitrogen (LN) without a previous partial dehydration process. The water content (WC) of the seeds at the moment of immersion in LN must be regarded as the most critical factor in cryopreservation. The purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of Citrus seeds: C. sinensis (sweet orange), C. paradisi (grapefruit), C. reticulata (mandarin) in LN. To study the tolerance to dehydration and LN exposure, seeds were desiccated by equilibration at relative humidities between 11 and 95%. Sorption isotherms were determined and modeled; lipid content of the seeds was measured. Seed desiccation sensitivity was quantified by the quantal response model. Differential scanning calorimetry (DSC) thermograms were determined on cotyledon tissue at different moisture contents to measure ice melting enthalpies and unfrozen WC. Samples of total seed lipid extract were also analyzed by DSC to identify lipid transitions in the thermograms. The limit of hydration for LN Citrus seeds treatment corresponded to the unfrozen WC in the tissue, confirming that seed survival strictly depended on avoidance of intracellular ice formation. Copyright © 2011 Society of Chemical Industry.

  16. Electrophysiology of pumpkin seeds: Memristors in vivo.

    Science.gov (United States)

    Volkov, Alexander G; Nyasani, Eunice K; Tuckett, Clayton; Greeman, Esther A; Markin, Vladislav S

    2016-01-01

    Leon Chua, the discoverer of a memristor, theoretically predicted that voltage gated ion channels can be memristors. We recently found memristors in different plants such as the Venus flytrap, Mimosa pudica, Aloe vera, apple fruits, and in potato tubers. There are no publications in literature about the existence of memristors in seeds. The goal of this work was to discover if pumpkin seeds might have memristors. We selected Cucurbita pepo L., cv. Cinderella, Cucurbita maxima L. cv Warty Goblin, and Cucurbita maxima L., cv. Jarrahdale seeds for this analysis. In these seeds, we found the presence of resistors with memory. The analysis was based on cyclic voltammetry where a memristor should manifest itself as a nonlinear two-terminal electrical element, which exhibits a pinched hysteresis loop on a current-voltage plane for any bipolar cyclic voltage input signal. Dry dormant pumpkin seeds have very high electrical resistance without memristive properties. The electrostimulation by bipolar sinusoidal or triangular periodic waves induces electrical responses in imbibed pumpkin seeds with fingerprints of memristors. Tetraethylammonium chloride, an inhibitor of voltage gated K(+) channels, transforms a memristor to a resistor in pumpkin seeds. NPPB (5-Nitro-2-(3-phenylpropylamino)benzoic acid) inhibits the memristive properties of imbibed pumpkin seeds. The discovery of memristors in pumpkin seeds creates a new direction in the understanding of electrophysiological phenomena in seeds.

  17. Germination of beans and snap beans seed

    Directory of Open Access Journals (Sweden)

    Zdravković Milan

    2000-01-01

    Full Text Available The aim of this study was to investigate germination of good bean seed of the variety Galeb and the bad bean seed of the same variety. We were also interested in germination of bean and snap bean seed damaged by grain weevil, and in germination of the seed treated by freezing which was aimed at controlling grain weevil by cold. We also recorded the differences between bean and snap bean seed, which was or was not treated by freezing in laboratory conditions. This investigation was carried out by applying the two factorial block system. The obtained results were evaluated by the variance analysis and x2 test These results suggest that the bean seed of a bad fraction had low levels of germination, but still it was present. Although the seed of good appearance was carefully selected, germination was slightly lower than it should have been. The seed with the large amount of grain weevils performed a high level germination in laboratory conditions. There were no differences in germination between the seed injured by grain weevil either in beans or in snap beans. As for the seed treated or untreated by freezing, there also were no differences between beans and snap beans. .

  18. Local evolution of seed flotation in Arabidopsis.

    Directory of Open Access Journals (Sweden)

    Susana Saez-Aguayo

    2014-03-01

    Full Text Available Arabidopsis seeds rapidly release hydrophilic polysaccharides from the seed coat on imbibition. These form a heavy mucilage layer around the seed that makes it sink in water. Fourteen natural Arabidopsis variants from central Asia and Scandinavia were identified with seeds that have modified mucilage release and float. Four of these have a novel mucilage phenotype with almost none of the released mucilage adhering to the seed and the absence of cellulose microfibrils. Mucilage release was modified in the variants by ten independent causal mutations in four different loci. Seven distinct mutations affected one locus, coding the MUM2 β-D-galactosidase, and represent a striking example of allelic heterogeneity. The modification of mucilage release has thus evolved a number of times independently in two restricted geographical zones. All the natural mutants identified still accumulated mucilage polysaccharides in seed coat epidermal cells. Using nuclear magnetic resonance (NMR relaxometry their production and retention was shown to reduce water mobility into internal seed tissues during imbibition, which would help to maintain seed buoyancy. Surprisingly, despite released mucilage being an excellent hydrogel it did not increase the rate of water uptake by internal seed tissues and is more likely to play a role in retaining water around the seed.

  19. Selected aspects of tiny vetch [Vicia hirsuta (L. Gray S.F.] seed ecology: generative reproduction and effects of seed maturity and seed storage on seed germination

    Directory of Open Access Journals (Sweden)

    Magdalena Kucewicz

    2012-12-01

    Full Text Available Vicia hirsuta (L. Gray S.F. (tiny vetch is a common and persistent segetal weed. Tiny vetch seeds and pods reach different stages of maturity during the crop harvest season. Some seeds that mature before cereal harvest are shed in the field and deposited in the soil seed bank, while others become incorporated into seed material. The objective of this study was to describe selected aspects of tiny vetch seed ecology: to determine the rate of individual reproduction of vetch plants growing in winter and spring grain crops and to evaluate the germination of seeds at different stages of maturity, subject to storage conditions. The seeds and pods of V. hirsuta were sorted according to their development stages at harvest and divided into two groups. The first group was stored under laboratory conditions for two months. In the autumn of the same year, the seeds were subjected to germination tests. The remaining seeds were stored in a storeroom, and were planted in soil in the spring. The germination rate was evaluated after 8 months of storage. Potential productivity (developed pods and flowers, fruit buds was higher in plants fruiting in winter wheat than in spring barley. Vetch plants produced around 17-26% more pods (including cracked, mature, greenish-brown and green pods and around 25% less buds in winter wheat than in spring barley. Immature seeds were characterized by the highest germination capacity. Following storage under laboratory conditions and stratification in soil, mature seeds germinated at a rate of several percent. After storage in a storeroom, seeds at all three development stages broke dormancy at a rate of 72- 75%. The high germination power of tiny vetch seeds stored in a storeroom indicates that this plant can be classified as an obligatory speirochoric weed species.

  20. Impurities in alfalfa seed and their impact on processing technology

    OpenAIRE

    Đokić, Dragoslav; Stanisavljević, Rade; Marković, Jordan; Terzić, Dragan; Anđelković, Bojan

    2012-01-01

    The aim of this research was to determine relevant parameters and optimal alfalfa seed processing technology by a comparative analysis of two systems of machinery for processing natural alfalfa seed of different purity (59.0% and 71.0%) and with different content of impurities. The relevant parameters monitored during the test were: pure seed (%), weed seeds and seeds of other crops (%), inert matter (%), amount of processed seed (kg), seed processing time (min), consumption of steel powder (...

  1. Effects of rodent species, seed species, and predator cues on seed fate

    Science.gov (United States)

    Sivy, Kelly J.; Ostoja, Steven M.; Schupp, Eugene W.; Durham, Susan

    2011-07-01

    Seed selection, removal and subsequent management by granivorous animals is thought to be a complex interaction of factors including qualities of the seeds themselves (e.g., seed size, nutritional quality) and features of the local habitat (e.g. perceived predator risk). At the same time, differential seed selection and dispersal is thought to have profound effects on seed fate and potentially vegetation dynamics. In a feeding arena, we tested whether rodent species, seed species, and indirect and direct predation cues influence seed selection and handling behaviors (e.g., scatter hoarding versus larder hoarding) of two heteromyid rodents, Ord's kangaroo rat ( Dipodomys ordii) and the Great Basin pocket mouse ( Perognathus parvus). The indirect cue was shrub cover, a feature of the environment. Direct cues, presented individually, were (1) control, (2) coyote ( Canis latrans) vocalization, (3) coyote scent, (4) red fox ( Vulpes vulpes) scent, or (5) short-eared owl ( Asio flammeus) vocalization. We offered seeds of three sizes: two native grasses, Indian ricegrass ( Achnatherum hymenoides) and bluebunch wheatgrass ( Pseudoroegneria spicata), and the non-native cereal rye ( Secale cereale), each in separate trays. Kangaroo rats preferentially harvested Indian ricegrass while pocket mice predominately harvested Indian ricegrass and cereal rye. Pocket mice were more likely to scatter hoard preferred seeds, whereas kangaroo rats mostly consumed and/or larder hoarded preferred seeds. No predator cue significantly affected seed preferences. However, both species altered seed handling behavior in response to direct predation cues by leaving more seeds available in the seed pool, though they responded to different predator cues. If these results translate to natural dynamics on the landscape, the two rodents are expected to have different impacts on seed survival and plant recruitment via their different seed selection and seed handling behaviors.

  2. Seed source, seed traits, and frugivore habits: Implications for dispersal quality of two sympatric primates.

    Science.gov (United States)

    Benítez-Malvido, Julieta; González-Di Pierro, Ana Ma; Lombera, Rafael; Guillén, Susana; Estrada, Alejandro

    2014-06-01

    • Premise of the study: Frugivore selection of fruits and treatment of seeds together with seed deposition site are crucial for the population dynamics of vertebrate-dispersed plants. However, frugivore species may influence dispersal quality differently even when feeding on the same fruit species and, while animals disperse some seeds, others simply fall beneath the parent plant.• Methods: In southern Mexico, we investigated to see if within-species seed traits (i.e., length, width, weight, and volume) and germination success differed according to seed source. For five tropical tree species we obtained ingested seeds from two sources, howler monkey (Alouatta pigra) and spider monkey (Ateles geoffroyi) feces; and noningested seeds from two sources, the ground and tree crowns (with predispersed seeds used as control).• Key results: A principal components' analysis showed that traits of seeds ingested by howler monkeys differed from other sources while seeds ingested by spider monkeys were similar to noningested seeds. Howlers consumed on average the larger seeds in Ampelocera hottlei, Brosimum lactescens, and Dialium guianense. Both primate species consumed the smaller seeds in Spondias mombin, while no seed trait differences among seed sources were found in Spondias radlkoferi. For all five tree species, germination rate was greatest for seeds ingested by howler monkeys.• Conclusions: For the studied plant species, seed ingestion by howler monkeys confers higher dispersal quality than ingestion by spider monkeys or nondispersal. Dispersal services of both primate species, however, are not redundant and may contribute to germination heterogeneity within plant populations in tropical forests. © 2014 Botanical Society of America, Inc.

  3. Yield and quality of white mustard seed depending on the seeding dates

    Directory of Open Access Journals (Sweden)

    Jevđović Radosav

    2000-01-01

    Full Text Available The effect of sowing dates and harvest dates on yield and quality of white mustard seed been investigated in this study. During the experiment two sowing dates have been tested the first one based on the crops in March, and the other one when the direct sowing was carried out in late May, after the harvest of camomile crops. The harvest of the seed crops in the first seeding date was performed on 1st July, and the second on 15th September. Seed fractioning extracted the following fractions: large (3mm, medium (2 mm and small (1mm. I he objective of the laboratory' experiments was to observe the effect of the seed fraction size on the most important parameters of its quality (germination energy, total germination and absolute mass according to the stipulated terms. Based on the data obtained in the survey, it can be concluded that the seed yield is significantly affected by the varied seeding dates. Earlier seeding date produced significantly increased seed yield (1920 kg/ha than the later seeding date (1160 kg/ha. According to the seed quality parameters, the best characteristics are found in the seed fraction of 3 mm, in both seeding and harvest dates.

  4. Activity uniformity of Ir-192 seeds

    International Nuclear Information System (INIS)

    Ling, C.C.; Gromadzki, Z.C.

    1981-01-01

    A simple device that uses materials and apparatus commonly available in a radiotherapy department has been designed, fabricated and used in routine quality control relative to the activity uniformity of clinical Ir-192 seeds in ribbons. Detailed evaluation indicated that this system is easy to use and can yield relative activity measurements of individual Ir-192 seeds accurate to within 2%. With this device, activity uniformity of commercial Ir-192 seeds from two manufacturers has been assessed. For the seven shipments of Ir-192 seeds studied, the root mean square variations of individual seed strength from the average of each shipment ranged from 3.4 to 7.1%. Variation in seed activity by more than +- 10% from the average is not uncommon

  5. Improving tomato seed quality- challenges and possibilities

    DEFF Research Database (Denmark)

    Shrestha, Santosh

    The thesis investigates the possibility of using single seed near-infrared (NIR) spectroscopy, multispectral imaging (MSI) and NIR hyperspectral imaging (NIR-HSI) in combination with chemometrics for rapid determination of the tomato seed quality. The results of the PhD study are compiled in four...... manuscripts (MS). These non-destructive methods show the potential of sorting tomato seeds as per their viability and varietal identity. The results are discussed in the context of possible contribution from these methods in the improvement of the seed quality in Nepal. In MS I, potential application of NIR...... spectroscopy in combination with chemometrics for prediction of tomato seed viability is demonstrated. The work in MS I also emphasises on identifying the important NIR spectral regions for the chemometric model that are relevant to the separation of viable and non-viable seeds. The NIR-HIS method was also...

  6. Commercial Scale Production of Mushroom Liquid Seeds

    International Nuclear Information System (INIS)

    Rosnani Abdul Rashid; Hassan Hamdani Hassan Mutaat; Mohd Meswan Maskom; Khairuddin Abdul Rahim

    2015-01-01

    Mushroom liquid seed production technology was developed by Malaysian Nuclear Agency (Nuclear Malaysia) in the late 1990s. Initially, the liquid seeds were used mainly in the solid state fermentation process for converting oil palm empty fruit bunch fibres into ruminant feed. Considering widespread problems encountered by mushroom growers from use of solid seeds, especially in cases of contaminant agents infecting cultivated bags and inconsistencies in yield, we diverted our focus to utilising liquid seeds as alternative inocula for mushroom cultivation. These problems provide us opportunities to look into the issues and address the problems faced by mushroom growers. However, the technology of producing liquid seed at laboratory scale needs to be primed for commercial production. This paper discusses developmental aspects of mushroom liquid seed at commercial scale for the advancement of the country's mushroom industry. (author)

  7. Chemical solution seed layer for rabits tapes

    Science.gov (United States)

    Goyal, Amit; Paranthaman, Mariappan; Wee, Sung-Hun

    2014-06-10

    A method for making a superconducting article includes the steps of providing a biaxially textured substrate. A seed layer is then deposited. The seed layer includes a double perovskite of the formula A.sub.2B'B''O.sub.6, where A is rare earth or alkaline earth metal and B' and B'' are different rare earth or transition metal cations. A superconductor layer is grown epitaxially such that the superconductor layer is supported by the seed layer.

  8. Effects of seed priming and water potential on seed germination and ...

    African Journals Online (AJOL)

    Administrator

    2011-05-23

    May 23, 2011 ... (without seed drying), primed and 12 h drying on seed germination of wheat .... completely with the lower half and the paper were rolled and placed ..... water stress and control of germination: a review. Plant Cell Environ.

  9. [Seed geography: its concept and basic scientific issues].

    Science.gov (United States)

    Yu, Shun-Li; Wang, Zong-Shuai; Zeren, Wangmu

    2010-01-01

    In this paper, a new concept 'seed geography' was provided, and its definition, research contents, and scientific issues were put forward. Seed geography is a newly developed interdisciplinary science from plant geography, seed ecology, and phytosociology, which studies the geographic variation patterns of seed biological traits as well as their relationships with environmental factors from macroscopic to microscopic, and the seed formation, development, and change trends. The main research contents would include geography of seed mass, geography of seed chemical components, geography of seed morphology, geography of seed cell biological characteristics, geography of seed physiological characteristics, geography of seed genetic characteristics, and geography of flower and fruit. To explore the scientific issues in seed geography would help us to better understand the long-term adaptation and evolution of seed characteristics to natural environments.

  10. [The research of Valeriana amurensis seed germination characteristics].

    Science.gov (United States)

    Liu, Juan; Yang, Chun-Rong; Jiang, Bo; Fang, Min; Du, Juan

    2011-10-01

    To study the effect of different treatments on the Valeriana amurensis seed germination rate. Used different chemical reagents and seed soakings on the routine germination test and the orthogonal test of the Valeriana amurensis seed, calculated the germination rate under different germination condition. Valeriana amurensis treated with different chemical reagends had different germination rate. The suitable immersion time could enhance Valeriana amurensis seed germination rate. Different treatment time, different disposal temperature, different germination temperature would have an impact on the Valeriana amurensis seed germination rate. In order to raise the Valeriana amurensis seed germination rate, use appropriate treatment on the seed before plant seeds; The seed growing must under suitable time and temperature.

  11. New design of experiment combined with UV-Vis spectroscopy for extraction and estimation of polyphenols from Basil seeds, Red seeds, Sesame seeds and Ajwan seeds

    Science.gov (United States)

    Mabood, Fazal; Gilani, Syed Abdullah; Hussain, Javid; Alshidani, Sulaiman; Alghawi, Said; Albroumi, Mohammed; Alameri, Saif; Jabeen, Farah; Hussain, Zahid; Al-Harrasi, Ahmed; Al Abri, Zahra K. M.; Farooq, Saima; Naureen, Zakira; Hamaed, Ahmad; Rasul Jan, M.; Shah, Jasmin

    2017-05-01

    New experimental designs for the extraction of polyphenols from different seeds including Basil seed, Red seed, Sesame seeds and Ajwan seeds were investigated. Four variables the concentration and volume of methanol and NaOH solutions as well as the temperature and time of extraction were varied to see their effect on total phenol extraction. The temperature was varied in the range from 25 °C to 200 °C while the time in the range from 30 to 200 minutes. Response surface methodology was used to optimize the extraction parameters. The estimation of polyphenols was measured through phenols reduction UV-Vis spectroscopic method of phosphotungstic-phosphomolybdic acids (Folin-Ciocalteu's reagent). Calibration curve was made by using tannic acid as a polyphenols standard in the concentration range from 0.1 to 10 ppm. The regression line obtained shows the value of correlation coefficient i.e. R = 0.930 and Root mean square error of cross validation (RMSEC) value of 0.0654. The Basil seeds were found containing the highest amount of total phenols i.e. 785.76 mg/100 g. While the Sesame seeds having the least amount i.e. 33.08 mg/100 g. The Ajwan seeds and the Red seeds are containing the medium amounts i.e. 379 mg/100 g and 220.54 mg/100 g respectively.

  12. Regulatory redox state in tree seeds

    Directory of Open Access Journals (Sweden)

    Ewelina Ratajczak

    2017-12-01

    Full Text Available Peroxiredoxins (Prx are important regulators of the redox status of tree seeds during maturation and long-term storage. Thioredoxins (Trx are redox transmitters and thereby regulate Prx activity. Current research is focused on the association of Trx with Prx in tree seeds differing in the tolerance to desiccation. The results will allow for better understanding the regulation of the redox status in orthodox, recalcitrant, and intermediate seeds. The findings will also elucidate the role of the redox status during the loss of viability of sensitive seeds during drying and long-term storage.

  13. New design of experiment combined with UV-Vis spectroscopy for extraction and estimation of polyphenols from Basil seeds, Red seeds, Sesame seeds and Ajwan seeds.

    Science.gov (United States)

    Mabood, Fazal; Gilani, Syed Abdullah; Hussain, Javid; Alshidani, Sulaiman; Alghawi, Said; Albroumi, Mohammed; Alameri, Saif; Jabeen, Farah; Hussain, Zahid; Al-Harrasi, Ahmed; Al Abri, Zahra K M; Farooq, Saima; Naureen, Zakira; Hamaed, Ahmad; Rasul Jan, M; Shah, Jasmin

    2017-05-05

    New experimental designs for the extraction of polyphenols from different seeds including Basil seed, Red seed, Sesame seeds and Ajwan seeds were investigated. Four variables the concentration and volume of methanol and NaOH solutions as well as the temperature and time of extraction were varied to see their effect on total phenol extraction. The temperature was varied in the range from 25°C to 200°C while the time in the range from 30 to 200minutes. Response surface methodology was used to optimize the extraction parameters. The estimation of polyphenols was measured through phenols reduction UV-Vis spectroscopic method of phosphotungstic-phosphomolybdic acids (Folin-Ciocalteu's reagent). Calibration curve was made by using tannic acid as a polyphenols standard in the concentration range from 0.1 to 10ppm. The regression line obtained shows the value of correlation coefficient i.e. R=0.930 and Root mean square error of cross validation (RMSEC) value of 0.0654. The Basil seeds were found containing the highest amount of total phenols i.e. 785.76mg/100g. While the Sesame seeds having the least amount i.e. 33.08mg/100g. The Ajwan seeds and the Red seeds are containing the medium amounts i.e. 379mg/100g and 220.54mg/100g respectively. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Patogenic fungi associated with blue lupine seeds

    Directory of Open Access Journals (Sweden)

    Bogdan Nowicki

    2013-12-01

    Full Text Available Over 10% ofseeds harvested in 1991 and 1992 (50 samples, 400 seeds in each sample proved to be infested with various fungi. Fusarium spp. and Botrytis cinerea were the most common pathogens isolated. Fusarium avenaceum was the most common and highIy pathogenic species. Fusarium semitectum and F. tricinctum were highly pathogenic to lupin seedlings but they were the least common Fusarium isolated from seeds. Similarily, Sclerotinia sclerotiorum was isolated only from 0,2% seeds tested but this fungus was highly pathogenic to lupin seedlings. Some other fungi know as lupin pathogens (F. oxysporum, Stemphylium botryosum, Pleiochaeta setosa and Phomopsis leptostromiformis were also noted in tested seeds.

  15. Occurrence of root parsley pathogens inhabiting seeds

    Directory of Open Access Journals (Sweden)

    Bogdan Nowicki

    2013-12-01

    Full Text Available The studies on root parsley pathogens inhabiting seeds were conducted during 1981-1988 and in 1993. Filter paper method with prefreezing and keeping under light was used. Each test sample comprised 500 seeds. Pathogenicity of collected fungal isolates was tested following two laboratory methods. 238 seed samples were studied. 18 fungal species were found but only 7 proved to be important pathogens of root parsley. The most common inhabitants of root parsley seeds were Alternaria spp. A.allernata occurred on 74,8% of seeds but only a few isolates showed to be slightly pathogenic while A.petroselini and A.radicina were higly pathogenic and inhabited 11,4 and 4,2% of seeds, respectively. The second group of important pathogens were species of Fusarium found on 3,9% of seeds. F.avenaceum dominated as it comprised 48% of Fusarium isolates, the next were as follow: F.culmorum - 20%, F.equiseti - 15%, F.solani - 8%, F.oxysporum - 7% and F.dimerum -2%. Some fungi like Botrytis cinerea, Septoria petroselini and Phoma spp. inhabited low number of seeds, respectively O,4; 0,5 and 0,8%, but they were highly pathogenic to root parsley. The fungi: Bipolaris sorokiniana, Drechslera biseptata, Stemphylium botryosum and Ulocludium consortiale showed slight pathogenicity. They were isolated from 3,8% of seeds.

  16. Microscale Insight into Microbial Seed Banks.

    Science.gov (United States)

    Locey, Kenneth J; Fisk, Melany C; Lennon, J T

    2016-01-01

    Microbial dormancy leads to the emergence of seed banks in environmental, engineered, and host-associated ecosystems. These seed banks act as reservoirs of diversity that allow microbes to persist under adverse conditions, including extreme limitation of resources. While microbial seed banks may be influenced by macroscale factors, such as the supply of resources, the importance of microscale encounters between organisms and resource particles is often overlooked. We hypothesized that dimensions of spatial, trophic, and resource complexity determine rates of encounter, which in turn, drive the abundance, productivity, and size of seed banks. We tested this using >10,000 stochastic individual based models (IBMs) that simulated energetic, physiological, and ecological processes across combinations of resource, spatial, and trophic complexity. These IBMs allowed realistic dynamics and the emergence of seed banks from ecological selection on random variation in species traits. Macroscale factors like the supply and concentration of resources had little effect on resource encounter rates. In contrast, encounter rates were strongly influenced by interactions between dispersal mode and spatial structure, and also by the recalcitrance of resources. In turn, encounter rates drove abundance, productivity, and seed bank dynamics. Time series revealed that energetically costly traits can lead to large seed banks and that recalcitrant resources can lead to greater stability through the formation of seed banks and the slow consumption of resources. Our findings suggest that microbial seed banks emerge from microscale dimensions of ecological complexity and their influence on resource limitation and energetic costs.

  17. Inheritance of seed coat color in sesame

    Directory of Open Access Journals (Sweden)

    Hernán Laurentin

    2014-04-01

    Full Text Available The objective of this work was to determine the inheritance mode of seed coat color in sesame. Two crosses and their reciprocals were performed: UCLA37 x UCV3 and UCLA90 x UCV3, of which UCLA37 and UCLA90 are white seed, and UCV3 is brown seed. Results of reciprocal crosses within each cross were identical: F1 seeds had the same phenotype as the maternal parent, and F2 resulted in the phenotype brown color. These results are consistent only with the model in which the maternal effect is the responsible for this trait. This model was validated by recording the seed coat color of 100 F2 plants (F3 seeds from each cross with its reciprocal, in which the 3:1 expected ratio for plants producing brown and white seeds was tested with the chi-square test. Sesame seed color is determined by the maternal genotype. Proposed names for the alleles participating in sesame seed coat color are: Sc1, for brown color; and Sc2, for white color; Sc1 is dominant over Sc2.

  18. Evaluation of seed vigor tests for determinig alfalfa seed quality

    Directory of Open Access Journals (Sweden)

    hamidreza tavakoli kakhki

    2009-06-01

    Full Text Available In order to determine the best seed vigor test in laboratory which may provide high correlation between alfalfa seedling stablishment in the field, two experiment were conducted in field as well as in laboratory. Four alfalfa cultivars Bami, Ghareyonjeh, Hamadani and Yazdi were used. Different seed vigor tests including standard germination, germination in low tempretaure (5c , germination rate, osmotic stress, accelerated ageing and electrical conductivity tests were laid out in a randomized completely design (RCD with four replications in laboratory experiment‌. The second experiment was carried out in a randomized completely block design (RCBD and each treatment replicated four times‌. Results revealed significant differences (p≤ 0.01 among different cultivars for establishment percentage and rate. Results also showed that there were significant correlations between electrical conductivity test and establishment percentage (r= -0.65 , p ≤ 0.01 as well as establishment rate (r= -0.80 , p ≤ 0.01. Correlation coefficients for rate and emergence percentage were significant and positive (r= 0.91, p ≤ 0.01 . Stepwise method disclosed that the regression model (y=165.23 – 0.15 x r2=0.64 , (p ≤ 0.01 for predicting rate of emergence could be recommended. In this model x (dependent variable is value of electrical conductivity and y (independent variable is rate of emergence.

  19. Chemical Characteristics of Pumpkin Seed Tempeh From Soybean and Pumpkin Seeds

    OpenAIRE

    Pujilestari, Shanti; Sandrasari, Diny A; Marida, Rimmaria

    2017-01-01

    The aim of this research was to find the effect of the combination of soybean and pumpkin seeds on the chemical characteristics of pumpkin seed tempeh. The pumpkin seed tempeh samples were analyzed for its water, ash, protein, fat, carbohydrate and crude fiber. Meanwhile, support data were isoflavone and zinc. Sensory hedonic was conducted by 25 untrained panelists for selecting the best formulation of soybean and pumpkin seeds in tempeh. The result shows the formulation of soybean and pumpki...

  20. Seed Placement in Permanent Breast Seed Implant Brachytherapy: Are Concerns Over Accuracy Valid?

    Energy Technology Data Exchange (ETDEWEB)

    Morton, Daniel, E-mail: dmorton@bccancer.bc.ca [Department of Medical Physics, BC Cancer Agency, Centre for the Southern Interior, Kelowna, British Columbia (Canada); Department of Physics and Astronomy, University of Victoria, Victoria, British Columbia (Canada); Hilts, Michelle [Department of Medical Physics, BC Cancer Agency, Centre for the Southern Interior, Kelowna, British Columbia (Canada); Department of Physics and Astronomy, University of Victoria, Victoria, British Columbia (Canada); Batchelar, Deidre [Department of Medical Physics, BC Cancer Agency, Centre for the Southern Interior, Kelowna, British Columbia (Canada); Crook, Juanita [Department of Radiation Oncology, BC Cancer Agency, Centre for the Southern Interior, Kelowna, British Columbia (Canada)

    2016-07-01

    Purpose: To evaluate seed placement accuracy in permanent breast seed implant brachytherapy (PBSI), to identify any systematic errors and evaluate their effect on dosimetry. Methods and Materials: Treatment plans and postimplant computed tomography scans for 20 PBSI patients were spatially registered and used to evaluate differences between planned and implanted seed positions, termed seed displacements. For each patient, the mean total and directional seed displacements were determined in both standard room coordinates and in needle coordinates relative to needle insertion angle. Seeds were labeled according to their proximity to the anatomy within the breast, to evaluate the influence of anatomic regions on seed placement. Dosimetry within an evaluative target volume (seroma + 5 mm), skin, breast, and ribs was evaluated to determine the impact of seed placement on the treatment. Results: The overall mean (±SD) difference between implanted and planned positions was 9 ± 5 mm for the aggregate seed population. No significant systematic directional displacements were observed for this whole population. However, for individual patients, systematic displacements were observed, implying that intrapatient offsets occur during the procedure. Mean displacements for seeds in the different anatomic areas were not found to be significantly different from the mean for the entire seed population. However, small directional trends were observed within the anatomy, potentially indicating some bias in the delivery. Despite observed differences between the planned and implanted seed positions, the median (range) V{sub 90} for the 20 patients was 97% (66%-100%), and acceptable dosimetry was achieved for critical structures. Conclusions: No significant trends or systematic errors were observed in the placement of seeds in PBSI, including seeds implanted directly into the seroma. Recorded seed displacements may be related to intrapatient setup adjustments. Despite observed seed

  1. Seed abnormalities and associated mycoflora of rain- fed wheat ...

    African Journals Online (AJOL)

    Owner

    seeds with discoloured embryo (germ) (1.2 – 1.5%) and brush (0.25 – 1.25%) ends. Fusarium graminearum and Helminthosporium sativum were associated with all seeds, ... Key words: Fungi, seed health testing, seed discolouration, wheat. INTRODUCTION. Abnormality in seeds is a major constraint in crop production in ...

  2. Nutritional value OF Bottle Gourd ( Lagenaria siceraria ) Seeds ...

    African Journals Online (AJOL)

    Whole seeds, dehulled seeds and seed coats of bottle gourd seed (Lagenaria siceraria) were analysed for their proximate, amino acids and mineral compositions. The results of the analysis showed that, whole seed has highest content of moisture (17.5 0.21%) and ash (5.80 0.83%) while dehulled had highest amount ...

  3. Use of linear discriminant function analysis in seed morphotype ...

    African Journals Online (AJOL)

    Variation in seed morphology of the Lima bean in 31 accessions was studied. Data were collected on 100-seed weight, seed length and seed width. The differences among the accessions were significant, based on the three seed characteristics. K-means cluster analysis grouped the 31 accessions into four distinct groups, ...

  4. Ecological correlates of seed survival after ingestion by Fallow Deer

    NARCIS (Netherlands)

    Mouissie, AM; Van der Veen, CEJ; Veen, GF; Van Diggelen, R

    1. The survival and retention of seeds was studied by feeding known quantities of seeds of 25 species to four captive Fallow Deer (Dama dama L.). To test for ecological correlates, plant species were selected to represent large variation in seed size, seed shape, seed longevity and habitat

  5. 7 CFR 201.57a - Dormant seeds.

    Science.gov (United States)

    2010-01-01

    ... REGULATIONS Germination Tests in the Administration of the Act § 201.57a Dormant seeds. Dormant seeds are viable seeds, other than hard seeds, which fail to germinate when provided the specified germination..., or application of germination promoting chemicals. (b) The percentage of dormant seed, if present...

  6. Seed regulation: choices on the road to reform

    NARCIS (Netherlands)

    Tripp, R.; Louwaars, N.P.

    1997-01-01

    Major changes in national seed systems, including the rapid development of commercial seed enterprises, the growth of non-governmental organization (NGO) seed projects, and the concomitant decline of public sector seed provision, call for a re-examination of seed regulatory frameworks in developing

  7. Conference Proceedings: Seed Ecology III - The Third International Society for Seed Science Meeting on Seeds and the Environment - "Seeds and Change"; June 20-June 24, 2010; Salt Lake City, Utah, USA

    Science.gov (United States)

    Rosemary Pendleton; Susan Meyer; Bitsy Schultz

    2010-01-01

    Seed Ecology III was held in Salt Lake City, Utah in June 2010, sharing the latest research on all aspects of seed ecology. Our meeting was organized around the theme "Seeds and Change." We welcomed contributions in any area of seed ecology. Our agenda also aimed to create bridges between seed ecology and plant conservation, restoration ecology, and global...

  8. Factors affecting the density of Brassica napus seeds

    NARCIS (Netherlands)

    Young, L.; Jalink, H.; Denkert, R.; Reaney, M.

    2006-01-01

    Brassica napus seed is composed of low density oil (0.92 g.cm(-3)) and higher density solids (1.3-1.45 g.cm(-3)). Seed buoyant density may potentially be used to determine seed oil content and to separate seeds with different oil contents, however, we have found that seeds with the lowest buoyant

  9. Lipids in citrus sinensis seeds

    International Nuclear Information System (INIS)

    Hamid, S.; Liaquat, L.; Khalid, B.; Khan, J.I.

    2003-01-01

    The seed oil of citrus sinensis when subjected to different physicochemical tests showed moisture 13.2%, ash 7.5%, ester value 1.29%, free fatty acid 0.4%. iodine value 65.0% and protein value 6.0%. According to lipid analysis. the oil was classified into hydrocarbons. wax esters, sterol esters, triglycerides. free fatty acids, 1,3 and 1,2 diglycerides, alcohols, sterols, monoglycerides, phosphatidylethanolamines, phosphatidylcholines and lysophosphatidylethanolamines. The fatty acid (C/sub 12.0/ - C/sub 21.0/) composition of all lipid classes was determined with the help of thin layer and gas liquid chromatography. (author)

  10. Seed size, shape and vertical distribution in the soil : indicators of seed longevity

    NARCIS (Netherlands)

    Bekker, RM; Bakker, JP; Grandin, U; Kalamees, R; Milberg, P; Poschlod, P; Thompson, K; Willems, JH

    1998-01-01

    1. We investigated the vertical distribution of seeds in the soil, using data from nine studies in five European countries. We discovered significant correlations between seed shape and distribution in the soil. 2. The classification of the longevity of seeds of plant species has been improved by

  11. Proanthocyanidins in seed coat tegmen and endospermic cap inhibit seed germination in Sapium sebiferum.

    Science.gov (United States)

    Shah, Faheem Afzal; Ni, Jun; Chen, Jing; Wang, Qiaojian; Liu, Wenbo; Chen, Xue; Tang, Caiguo; Fu, Songling; Wu, Lifang

    2018-01-01

    Sapium sebiferum , an ornamental and bio-energetic plant, is propagated by seed. Its seed coat contains germination inhibitors and takes a long time to stratify for germination. In this study, we discovered that the S. sebiferum seed coat (especially the tegmen) and endospermic cap (ESC) contained high levels of proanthocyanidins (PAs). Seed coat and ESC removal induced seed germination, whereas exogenous application with seed coat extract (SCE) or PAs significantly inhibited this process, suggesting that PAs in the seed coat played a major role in regulating seed germination in S. sebiferum . We further investigated how SCE affected the expression of the seed-germination-related genes. The results showed that treatment with SCE upregulated the transcription level of the dormancy-related gene, gibberellins (GAs) suppressing genes, abscisic acid (ABA) biosynthesis and signalling genes. SCE decreased the transcript levels of ABA catabolic genes, GAs biosynthesis genes, reactive oxygen species genes and nitrates-signalling genes. Exogenous application of nordihydroguaiaretic acid, gibberellic acid, hydrogen peroxide and potassium nitrate recovered seed germination in seed-coat-extract supplemented medium. In this study, we highlighted the role of PAs, and their interactions with the other germination regulators, in the regulation of seed dormancy in S. sebiferum .

  12. Supplementing seed banks to rehabilitate disturbed Mojave Desert shrublands: where do all the seeds go?

    Science.gov (United States)

    DeFalco, Lesley A.; Esque, Todd C.; Nicklas, Melissa B.; Kane, Jeffrey M.

    2012-01-01

    Revegetation of degraded arid lands often involves supplementing impoverished seed banks and improving the seedbed, yet these approaches frequently fail. To understand these failures, we tracked the fates of seeds for six shrub species that were broadcast across two contrasting surface disturbances common to the Mojave Desert—sites compacted by concentrated vehicle use and trenched sites where topsoil and subsurface soils were mixed. We evaluated seedbed treatments that enhance soil-seed contact (tackifier) and create surface roughness while reducing soil bulk density (harrowing). We also explored whether seed harvesting by granivores and seedling suppression by non-native annuals influence the success of broadcast seeding in revegetating degraded shrublands. Ten weeks after treatments, seeds readily moved off of experimental plots in untreated compacted sites, but seed movements were reduced 32% by tackifier and 55% through harrowing. Harrowing promoted seedling emergence in compacted sites, particularly for the early-colonizing species Encelia farinosa, but tackifier was largely ineffective. The inherent surface roughness of trenched sites retained three times the number of seeds than compacted sites, but soil mixing during trench development likely altered the suitability of the seedbed thus resulting in poor seedling emergence. Non-native annuals had little influence on seed fates during our study. In contrast, the prevalence of harvester ants increased seed removal on compacted sites, whereas rodent activity influenced removal on trenched sites. Future success of broadcast seeding in arid lands depends on evaluating disturbance characteristics prior to seeding and selecting appropriate species and seasons for application.

  13. Evaluation of non-chemical seed treatment methods for control of Alternaria brassicicola on cabbage seeds

    NARCIS (Netherlands)

    Amein, T.; Wright, Al S.; Wikstrom, M.; Koch, E.; Schmitt, A.; Stephan, D.; Jahn, M.; Tinivella, F.; Gullino, M.L.; Forsberg, G.; Werner, S.; Wolf, van der J.M.; Groot, S.P.C.

    2011-01-01

    Due to the lack of foliar fungicide use, the organic production of Brassica seeds free of Alternaria spp. is difficult. Therefore, effective seed treatments certified for use in organic farming are needed to eradicate or at least effec­tively reduce the seed-borne inoculum. We here report results of

  14. Bulk YBCO seeded with 45°–45° bridge-seeds of different lengths

    International Nuclear Information System (INIS)

    Shi, Y-H; Durrell, J H; Dennis, A R; Cardwell, D A

    2013-01-01

    Single grain, (RE)BCO (rare earth–barium–copper oxide) bulk superconductors in large or complicated geometries are required for a variety of potential applications, such as in motors and generators and magnetic shielding devices. As a result, top, multi-seeded, melt growth has been investigated over the past 15 years in an attempt to enlarge the size of (RE)BCO single grains specifically for such applications. Of these multi-seeding techniques, so-called bridge-seeding provides the best alignment of two seeds in a single grain growth process. Here we report, for the first time, the successful growth of YBCO (yttrium–barium–copper oxide) using a special, 45°–45°, arrangement of bridge-seeds. The superconducting properties, including trapped field, of the multi-seeded YBCO grains have been measured for different bridge lengths of the 45°–45° bridge-seeds. The boundaries at the impinging growth front and the growth features of the top, multi-seeded surface and cross-section of the multi-seeded, samples have been analysed using optical microscopy. The results suggest that an impurity-free boundary between the two seeds of each leg of the bridge-seed can form when 45°–45° bridge-seeds are used to enlarge the size of YBCO grains. (paper)

  15. Estimating Cone and Seed Production and Monitoring Pest Damage in Southern Pine Seed Orchards

    Science.gov (United States)

    Carl W. Fatzinger; H. David Muse; Thomas Miller; Helen T. Bhattacharyya

    1988-01-01

    Field sampling procedures and computer programs are described for monitoring seed production and pest damage in southern pine seed orchards. The system estimates total orchard yields of female strobili and seeds, quantifies pest damage, determines times of year when losses occur, and produces life tables for female strobili. An example is included to illustrate the...

  16. Farmers' seed sources and seed quality: 1. Physical and physiological quality

    NARCIS (Netherlands)

    Bishaw, Z.; Struik, P.C.; Gastel, van A.J.G.

    2012-01-01

    Understanding farmers' seed quality problem will enable farmers to devise strategies to improve quality at the farm level. The study was conducted to assess the quality of seed used by farmers from different sources and regions. A total of 304 wheat (Trticium aestivum L. and T. durum L.) seed

  17. Proanthocyanidins in seed coat tegmen and endospermic cap inhibit seed germination in Sapium sebiferum

    Directory of Open Access Journals (Sweden)

    Faheem Afzal Shah

    2018-04-01

    Full Text Available Sapium sebiferum, an ornamental and bio-energetic plant, is propagated by seed. Its seed coat contains germination inhibitors and takes a long time to stratify for germination. In this study, we discovered that the S. sebiferum seed coat (especially the tegmen and endospermic cap (ESC contained high levels of proanthocyanidins (PAs. Seed coat and ESC removal induced seed germination, whereas exogenous application with seed coat extract (SCE or PAs significantly inhibited this process, suggesting that PAs in the seed coat played a major role in regulating seed germination in S. sebiferum. We further investigated how SCE affected the expression of the seed-germination-related genes. The results showed that treatment with SCE upregulated the transcription level of the dormancy-related gene, gibberellins (GAs suppressing genes, abscisic acid (ABA biosynthesis and signalling genes. SCE decreased the transcript levels of ABA catabolic genes, GAs biosynthesis genes, reactive oxygen species genes and nitrates-signalling genes. Exogenous application of nordihydroguaiaretic acid, gibberellic acid, hydrogen peroxide and potassium nitrate recovered seed germination in seed-coat-extract supplemented medium. In this study, we highlighted the role of PAs, and their interactions with the other germination regulators, in the regulation of seed dormancy in S. sebiferum.

  18. Incorporating seeds in activated carbon pellets limits herbicide effects to seeded bunchgrasses when controlling exotic annuals

    Science.gov (United States)

    Revegetation of exotic annual grass-invaded rangeland with pre-emergent herbicides is challenging because seeding is delayed until herbicide toxicity has diminished, but at this time, exotic annuals can be re-invading. Incorporating seeds into activated carbon pellets may allow seeding to occur at t...

  19. In vitro Seed-dressing Technique for the Control of Seed-borne ...

    African Journals Online (AJOL)

    In vitro Seed-dressing Technique for the Control of Seed-borne Fungi of Rice variety Faro -29. ... Journal of Applied Sciences and Environmental Management ... Fernasan-D, Apron Plus 50 DS and Dithane–M45), and soaking and slurry methods at various concentrations, for the control of seed-borne fungi of rice variety

  20. Local Seed Business in the Context of Integrated Seed Sector Development

    NARCIS (Netherlands)

    Thijssen, M.H.; Borman, G.D.; Verhoosel, K.S.; Mastenbroek, A.; Heemskerk, Willem

    2015-01-01

    Quality seed is a key input for agriculture, with a direct impact on agricultural production and productivity. Integrated seed sector development (ISSD) is an inclusive approach that recognizes and builds upon a diversity of seed systems in the sector. We use the ISSD approach to guide us in the

  1. ESTIMATION OF EFFICIENCY OF MACHINERY FOR PRE-SOWING TREATMENT OF RADISH SEEDS FOR SEED PRODUCTION

    Directory of Open Access Journals (Sweden)

    S. M. Sirota

    2016-01-01

    Full Text Available The results of pre-sowing treatment of radish seeds aimed at increasing of seed production, yield and its productivity in protected area are presented. The density fractionation of radish seeds by gravity separator is recommended for improvement of planting material quality and increasing of utilization efficiency of frame area.

  2. Genetic analysis of seed-soluble oligosaccharides in relation to seed storability of Arabidopsis

    NARCIS (Netherlands)

    Bentsink, L.; Alonso-Blanco, C.; Vreugdenhil, D.; Tesnier, K.; Groot, S.P.C.; Koornneef, M.

    2000-01-01

    Seed oligosaccharides (OSs) and especially raffinose series OSs (RSOs) are hypothesized to play an important role in the acquisition of desiccation tolerance and consequently in seed storability. In the present work we analyzed the seed-soluble OS (sucrose, raffinose, and stachyose) content of

  3. Recent trends in post-wildfire seeding in western US forests: costs and seed mixes

    Science.gov (United States)

    Donna L. Peppin; Peter Z. Fule; Carolyn Hull Sieg; Jan L. Beyers; Molly E. Hunter; Pete Robichaud

    2011-01-01

    Broadcast seeding is one of the most commonly used post-fire rehabilitation treatments to establish ground cover for erosion control and mitigation of non-native plant species invasions. Little quantitative information is available on overall trends of post-fire seeding expenditures and seed mixes used over time in forested ecosystems in the western USA. We reviewed...

  4. Potential of endozoochorous seed dispersal by sheep in calcareous grasslands: correlations with seed traits.

    NARCIS (Netherlands)

    Kuiters, A.T.; Huiskes, H.P.J.

    2010-01-01

    Questions: What is the potential of sheep to serve as seed dispersers via ingestion and defecation in calcareous grasslands? Is the presence of viable seeds from dung correlated with specific seed traits? Location: Calcareous grasslands, South Limburg, the Netherlands/Belgium. Methods: Dung samples

  5. Emergence of native plant seeds in response to seed pelleting, planting depth, scarification, and soil anti-crusting treatment, 2009

    Science.gov (United States)

    Clint Shock; Erik Feibert; Lamont Saunders; Nancy Shaw

    2010-01-01

    Seed of native plants is needed to restore rangelands of the Intermountain West. Reliable commercial seed production is desirable to provide the quantity of seed needed for restoration efforts. Establishment of native seed crops has been difficult, because fall-planted seed is susceptible to bird damage, soil crusting, and soil erosion. Fall planting is important for...

  6. Plasticity of seed weight compensates reductions in seed number of oilseed rape in response to shading at flowering

    NARCIS (Netherlands)

    Labra Fernandez, Marcelo; Struik, Paul C.; Evers, Jochem B.; Calderini, Daniel F.

    2017-01-01

    Understanding the response of the number of seeds and seed weight to the availability of assimilates is crucial for designing breeding strategies aimed to increase seed and oil yield in oilseed rape. This study aims to answer the questions: i) do seed number and seed weight in oilseed rape differ

  7. Energy cost of seed drying

    Directory of Open Access Journals (Sweden)

    Weerachet Jittanit

    2017-11-01

    Full Text Available In this work, the energy costs of drying corn, rice and wheat seeds between 3 drying options were compared. They consisted of 1 two-stage drying by using fluidised bed dryer (FBD in the 1st stage and in-store dryer (ISD in the 2nd stage, 2 single-stage drying by fixed bed dryer (FXD and 3 two-stage drying by using FXD in the 1st  stage and ISD in the 2nd  stage. The drying conditions selected for comparison were proved to be safe for seed viability by the previous studies. The results showed that the drying options 2 and 3 consumed less energy than option 1. However, the benefits from lower energy cost must be weighed against some advantages of using FBD. Furthermore, it appeared that running the burners of FXD and ISD for warming up the ambient air during humid weather condition could shorten drying time significantly with a little higher energy cost.

  8. Seed Anatomy and Water Uptake in Relation to Seed Dormancy in Opuntia tomentosa (Cactaceae, Opuntioideae)

    Science.gov (United States)

    Orozco-Segovia, A.; Márquez-Guzmán, J.; Sánchez-Coronado, M. E.; Gamboa de Buen, A.; Baskin, J. M.; Baskin, C. C.

    2007-01-01

    Background and Aims There is considerable confusion in the literature concerning impermeability of seeds with ‘hard’ seed coats, because the ability to take up (imbibe) water has not been tested in most of them. Seeds of Opuntia tomentosa were reported recently to have a water-impermeable seed coat sensu lato (i.e. physical dormancy), in combination with physiological dormancy. However, physical dormancy is not known to occur in Cactaceae. Therefore, the aim of this study was to determine if seeds of O. tomentosa are water-permeable or water-impermeable, i.e. if they have physical dormancy. Methods The micromorphology of the seed coat and associated structures were characterized by SEM and light microscopy. Permeability of the seed-covering layers was assessed by an increase in mass of seeds on a wet substrate and by dye-tracking and uptake of tritiated water by intact versus scarified seeds. Key Results A germination valve and a water channel are formed in the hilum–micropyle region during dehydration and ageing in seeds of O. tomentosa. The funicular envelope undoubtedly plays a role in germination of Opuntia seeds via restriction of water uptake and mechanical resistance to expansion of the embryo. However, seeds do not exhibit any of three features characteristic of those with physical dormancy. Thus, they do not have a water-impermeable layer(s) of palisade cells (macrosclereids) or a water gap sensu stricto and they imbibe water without the seed coat being disrupted. Conclusions Although dormancy in seeds of this species can be broken by scarification, they have physiological dormancy only. Further, based on information in the literature, it is concluded that it is unlikely that any species of Opuntia has physical dormancy. This is the first integrative study of the anatomy, dynamics of water uptake and dormancy in seeds of Cactaceae subfamily Opuntioideae. PMID:17298989

  9. Defatted Detarium senegalense seed-based diet alters lipid profile ...

    African Journals Online (AJOL)

    Defatted Detarium senegalense seed-based diet alters lipid profile, ... cheaper alternative source for good quality protein for dietary purposes, we evaluated Detarium ... Whole seed residue, DDS seed flour and control diets (soybeans) were ...

  10. Sorghum [Sorghum bicolor (L.) Moench] Seed Quality as Affected by ...

    African Journals Online (AJOL)

    ... bicolor (L.) Moench seeds subjected to different field cultural management practices. ... Germination and vigour tests indicated that seed selection time did not ... In relation to this, variety E1291 showed better seed vigour, viability and yield ...

  11. Economic performance of community based bean seed production

    African Journals Online (AJOL)

    ACSS

    2017-05-08

    May 8, 2017 ... in rural areas and those in farmer organisations/cooperatives. However, seed production through this model is concentrated closer to urban areas, where individual seed producers are easily linked to the formal seed system.

  12. heritability and number of genes controlling seed yield in bottle ...

    African Journals Online (AJOL)

    USER

    2018-05-10

    May 10, 2018 ... For seed yield per plant, 100-seed weight per fruit, and number of seeds per fruit, a positive hypothetical heterosis was observed when calabash type was a maternal parent. ...... genes controlled fruit weight in watermelon.

  13. on seed germination and growth of Garcinia kola

    African Journals Online (AJOL)

    SARAH

    2016-07-31

    Jul 31, 2016 ... Seed germination tests: After 72 h of fermentation in plastic bags, seeds were ... Models (GLM) procedure of the R statistical version 9.1 was used to identify traits .... L-1) had accelerated seed germination. Germination rates.

  14. Increasing storability of Ceratoides arborescens seeds in ultradry ...

    African Journals Online (AJOL)

    USER

    2010-07-19

    Jul 19, 2010 ... After ultradrying, aging was accelerated in the seeds (45°C,. 2 days), and some .... According to International Rules for Seed Testing (ISTA, 1993). Seed moisture content was ... Statistical analysis. Data were subjected to ...

  15. EFFECTS OF NATURAL LONG STORAGE DURATION ON SEED ...

    African Journals Online (AJOL)

    lep

    2013-04-10

    Apr 10, 2013 ... germination and viability, seed vigor index and seed vigor under a 7.3% moisture content when .... containing two disks of Whatman filter papers soaked in distilled water. ..... desiccation tolerance (Review Update). Seed Sci.

  16. STATIONARY\\MOBILE SEEDS EXTRACTOR FROM FRUITS OF CUCURBIT CROPS

    Directory of Open Access Journals (Sweden)

    P. N. Tokarev

    2014-01-01

    Full Text Available The technical specifications and test data in 2012-2014 of a new fixed-mobile seed extractor of pumpkin and squash seeds (varieties of different ripeness, size and shape of fruit and seeds are presented.

  17. Effects of Seed Size on Germination and Early Morphorlogical and ...

    African Journals Online (AJOL)

    Effects of Seed Size on Germination and Early Morphorlogical and Physiological Characteristics of Gmelina Arborea , Roxb. ... African Research Review ... They were grouped into 3 categories as large seed size (LSS), medium seed size ...

  18. Tea seed upgrading facilities and economic assessment of biodiesel production from tea seed oil

    Energy Technology Data Exchange (ETDEWEB)

    Demirbas, Ayhan [Sirnak University, Engineering Faculty, Sirnak (Turkey); Sila Science, Trabzon (Turkey)

    2010-12-15

    Green tea seed (Camellia sinensis L. Kuntze) oil was used in this work. The tea seed oil contains more than 84% unsaturated fatty acid, such as oleic acid (62.5% by weight), linoleic acid (18.1% by weight) and linolenic acid. The biodiesel from tea seed oil in itself is not significantly different from biodiesel produced from vegetable oils. However, tea seed oil has lower pour point and lower viscosity as different common vegetable oils. Crude tea seed oil is one of the cheapest vegetable oil feedstocks with average price, 514 (US$/ton). (author)

  19. Tea seed upgrading facilities and economic assessment of biodiesel production from tea seed oil

    International Nuclear Information System (INIS)

    Demirbas, Ayhan

    2010-01-01

    Green tea seed (Camellia sinensisL. Kuntze) oil was used in this work. The tea seed oil contains more than 84% unsaturated fatty acid, such as oleic acid (62.5% by weight), linoleic acid (18.1% by weight) and linolenic acid. The biodiesel from tea seed oil in itself is not significantly different from biodiesel produced from vegetable oils. However, tea seed oil has lower pour point and lower viscosity as different common vegetable oils. Crude tea seed oil is one of the cheapest vegetable oil feedstocks with average price, 514 (US$/ton).

  20. processing of fluted pumpkin seeds, telfairia occidentalis

    African Journals Online (AJOL)

    ismail - [2010

    anti-nutritional factors in the seeds as well as growth and animal metabolism. Fresh seeds of ..... Adeyemi of the Department of Anatomy; Mrs. E.A. Adebiyi and O. Olatoye of the. Department .... China Agricultural University: Beijing, China. 2003 ...

  1. Seed bank characteristics of Dutch plant communities

    NARCIS (Netherlands)

    Bekker, RM; Schaminee, JHJ; Bakker, JP; Thompson, K

    With the recent appearances of a new and well-documented classification of the Dutch plant communities (Schaminee et al 1995a,b; 1996) and a database on the seed longevity of plant species of North West Europe (Thompson ct al. 1997a) it was possible to investigate patterns of seed longevity in Dutch

  2. Evaluating cotton seed gland initiation by microscopy

    Science.gov (United States)

    Gossypol is a terpenoid aldehyde found in cotton (Gossypium hirsutum L.) glands and helps protect the seed from pests and pathogens. However, gossypol is toxic to many animals, so the seed is used mainly in cattle feed, as ruminants are tolerant to the effects of gossypol. In order to develop strat...

  3. Sycamore produces viable seed after six years

    Science.gov (United States)

    A. F. Ike

    1966-01-01

    In the early stages of any tree improvement program it is desirable to know how soon progenies of selected parents can themselves be included in a breeding program. How soon will they produce viable pollen and seed? In the case of sycamore (Platanus occidentalis L.), the information is meager: the Woody- Plant Seed Manual lists the minimum commercial seedbearing age...

  4. Ants, rodents and seed predation in Proteaceae

    African Journals Online (AJOL)

    ... their nests extremely rapidly. One benefit of the ant-plant interaction may be seed escape ... (odourless to humans when dry) household glue or, b) placing seed in a Petri dish ... the layout of exclosures was completed. Response was not as.

  5. Study of seed for synthetical quartz

    International Nuclear Information System (INIS)

    Suzuki, C.K.; Torikai, D.

    1988-01-01

    Natural quartz blocks for seed (synthetic quartz technology) were studied by using various characterization techniques, such as X-ray topography, optical micrography, inspectoscopy, polariscopy and conoscopy, and etching. One of the most commonly found defect is the electrical or Dauphine twin. In The present research, we have developed a methodology to obtain a highly perfect seed for the synthetic quartz industries. (author) [pt

  6. Variation for seed phytosterols in sunflower germplasm

    Science.gov (United States)

    Sunflower (Helianthus annuus L.) seeds and oils are rich sources of phytosterols, which are important compounds for human nutrition. There is limited information on variability for seed phytosterols in sunflower germplasm. The objective of the present research was to evaluate kernel phytosterol cont...

  7. UTILISATION OF RUBBER SEED OL IN THE

    African Journals Online (AJOL)

    Thermal degradation studies on polyvinyl chloride in the presence of rubber seed oil, epoxidized rubber seed oil and metal ... Epoxidation of RSO was carried out at 29°C using peractic acid ..... impurities such as pigments, Vitamins, sterols ...

  8. Radiosensitivity of quince seeds (Cydonia oblonga Mill.)

    International Nuclear Information System (INIS)

    Dall'Orto, F.A.C.; Ojima, M.; Hiroce, R.; Igue, T.; Ferraz, E.S.B.; Nascimento Filho, V.F. do; Menten, J.O.M.; Tulmann Neto, A.; Ando, A.

    1984-01-01

    The investigation with quince seeds (Cydonia oblonga Mill.) radiosensitivity and the mineral composition of the plants obtained for mutation breeding are related. The concentration of some macro and micronutrients in quince seedlings obtained from irradiated seeds are studied. (M.A.C.) [pt

  9. Functionality-driven fractionation of lupin seeds

    NARCIS (Netherlands)

    Berghout, J.A.M.

    2015-01-01

    Functionality-driven fractionation of lupin seeds

    The growth in the world population requires an increase in the production of protein-rich foods from plant-based materials. Lupin seeds have potential to become a novel plant protein source for food products because they are rich

  10. Proteomics of Arabidopsis seed germination and priming

    NARCIS (Netherlands)

    Gallardo, K.; Job, C.; Groot, S.P.C.; Puype, M.; Demol, H.; Vandekerckhove, J.; Job, D.

    2003-01-01

    To better understand seed germination, a complex developmental process, we developed a proteome analysis of the model plant Arabidopsis for which complete genome sequence is now available. Among about 1,300 total seed proteins resolved in two-dimensional gels, changes in the abundance (up- and

  11. Regulation of seasonal patterns in seed dormancy

    NARCIS (Netherlands)

    Derkx, M.P.M.

    1993-01-01

    Buried seeds of many wild species pass annually through a pattern of induction and release of dormancy. These reversible changes in dormancy may be repeated for numbers of years when seeds are deprived from light and other germination-stimulating factors, and are a highly useful adaptation

  12. Dormancy Breaking in Ormosia arborea Seeds

    Directory of Open Access Journals (Sweden)

    Edilma Pereira Gonçalves

    2011-01-01

    Full Text Available Ormosia arborea is a tree species planted in urban areas and used to restore degraded areas. Its seeds are dormant and propagation is difficult. This study compares different dormancy breaking methods and physiological seed quality and seedling production. The seeds were germinated in sand in the laboratory of the Universidade Estadual de Santa Cruz, Ilhéus, Bahia, Brazil. The following dormancy breaking treatments were applied: control (intact seeds, 100°C water immersion; boiling water immersion followed by 24 hours of soaking; scarification with number 100 and number 50 sandpaper opposite from root emergence; sulfuric acid immersion for 1 hour, 50, 45, and 30 minutes. Seed immersion in 100°C and boiling water did not break the dormancy. The study species showed a greater vigor of seedling when its seeds were submitted to treatments associated with tegument rupturing by sandpaper or sulfuric acid. On the other hand, seed scarification with sulfuric acid for 1 hour, 50, 45, and 30 minutes or sandpaper favored seed germination and vigor.

  13. Elastohydrodynamic Traction Properties of Seed Oils

    Science.gov (United States)

    The elastohydrodynamic traction coefficient (tc) properties of nine seed oils of varying chemical structures, PAO and hexadecane, were investigated using a ball-on disk traction apparatus. The seed oils were: castor oil, a triglyceride with hydroxyl functional group; jojoba, a monoglyceride; and s...

  14. Elastohydrodynamic (EHD) traction properties of seed oils

    Science.gov (United States)

    The elastohydrodynamic traction coefficient (tc) properties of nine seed oils of varying chemical structures, PAO and hexadecane, were investigated using a ball-on disk traction apparatus. The seed oils were: castor oil, a triglyceride with hydroxyl functional group; jojoba, a monoglyceride; and sev...

  15. STOVE: Seed treatments for organic vegetable production

    NARCIS (Netherlands)

    Schmitt, A.; Jahn, M.; Kromphardt, C.; Krauthausen, H.J.; Roberts, S.J.; Wright, S.A.I.; Amein, T.; Forsberg, G.; Tinivella, F.; Gullino, M.L.; Wikström, M.; Wolf, van der J.M.; Groot, S.P.C.; Werner, S.; Koch, E.

    2008-01-01

    The aim of the EU-financed research project „STOVE“ (Seed Treatments for Organic Vegetable Production) is to evaluate different methods potentially suited for seed treatment of vegetables in organic farming regarding their efficacy, to optimise these methods, and where feasible to combine them with

  16. Stimulation of seeds by low dose irradiation

    International Nuclear Information System (INIS)

    Lawson, Helen

    1976-05-01

    The first section of the bibliography lists materials on the stimulation of seeds by low dose irradiation, with particular reference to stimulation of germination and yield. The second section contains a small number of selected references on seed irradiation facilities. (author)

  17. Transport processes in pea seed coats

    NARCIS (Netherlands)

    Dongen, Joost Thomas van

    2001-01-01

    The research described in this thesis concerns transport processes in coats of developing pea seeds. The scope of the investigation ranges from seed coat anatomy, via transport studies to the cloning of cDNA encoding proteinaceous membrane pores, and the heterologous expression of these

  18. Some insects affecting Penstemon seed production

    Science.gov (United States)

    Robert Hammon; Melissa Franklin

    2012-01-01

    Beardtongue (Penstemon Schmidel [Scrophulariaceae)) seeds are often produced without apparent damage from pests, but several species of native insects can adversely impact seed production fields. Tarnished plant bug (Lygus lineolaris (Palisot)) and western plant bug (Lygus hesperus Knight [Hemiptera: Miridae]), penstemon weevil (Hesperobaris sp. Casey [Coleoptera:...

  19. Electromagnetic treatment of loblolly pine seeds

    Energy Technology Data Exchange (ETDEWEB)

    Barnett, J. P. [Southern Forest Experiment Station, New Orleans, LA (United States); Krugman, S. L.

    1989-11-15

    Loblolly pine (Pinus faeda L.) seeds were exposed to an electromagnetic radiation treatment (Energy Transfer Process@, marketed by the Energy Transfer Corporation), and the effects of the treatments on seed germination, seedling development, disease resistance, and field performance of seedlings were evaluated. None of the evaluated variables showed any improvement over untreated controls.

  20. Puncturevine seed response to postemergence herbicides

    Science.gov (United States)

    Puncturevine (Tribulus terrestris) continues to plague growers, vegetation managers, and home gardeners. Puncturevine is a summer annual weed with spiny fruits that split into five segments, each containing one to four seeds. The seed nearest the pointed end of the bur is the largest and usually ge...

  1. Iodine-125 seeds for cancer treatment

    Energy Technology Data Exchange (ETDEWEB)

    Rostelato, Maria E.C.M.; Zeituni, Carlos A.; Feher, Anselmo; Moura, Joao A.; Moura, Eduardo S.; Nagatomi, Helio R.; Manzoli, Jose E.; Souza, Carla D., E-mail: elisaros@ipen.b, E-mail: czeituni@pobox.co, E-mail: afeher@ipen.b, E-mail: jmoura31@yahoo.com.b, E-mail: esmoura@ipen.b, E-mail: hrnagato@ipen.b, E-mail: jemanzoli@ipen.b, E-mail: cdsouza@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Karam, Dib, E-mail: dib.karan@usp.b [Universidade de Sao Paulo (USP), SP (Brazil). Escola de Artes, Ciencias e Humanidades

    2009-07-01

    In Brazil, cancer has become one of the major public health problems. An estimate by the Health Ministry showed that 466,430 people had the disease in the country in 2008. The prostate cancer is the second largest death cause among men. The National Institute of Cancer estimated the occurrence of 50,000 new cases for 2009. Some of these patients are treated with Brachytherapy, using Iodine-125 seeds. By this technique, small seeds with Iodine-125, a radioactive material, are implanted in the prostate. The advantages of radioactive seed implants are the preservation of healthy tissues and organs near the prostate, besides the low rate of impotence and urinary incontinence. The Energy and Nuclear Research Institute - IPEN, which belongs to the Nuclear Energy National Commission - CNEN, established a program for the development of the technique and production of Iodine-125 seeds in Brazil. The estimate for the 125-Iodine seeds demand is of 8,000 seeds/month and the laboratory to be implanted will need this production capacity. The purpose of this paper is to explain the project status and show some data about the seeds used in the country. The project will be divided in two phases: technological development of a prototype and a laboratory implementation for the seeds production. (author)

  2. Imazapyr (herbicide) seed dressing increases yield, suppresses ...

    African Journals Online (AJOL)

    from damage. In 1998/99 season, a trial was initiated at Chitedze Research Station under artificial infection, to evaluate the effects of seed dressing with imazapyr (an acetolactate synthase {ALS} inhibiting herbicide) using three seed treatment methods (coating, priming or drenching) and three herbicide rates (15, 30 and 45 ...

  3. Seed rain, soil seed bank, seed loss and regeneration of Castanopsis fargesii (Fagaceae) in a subtropical evergreen broad-leaved forest

    Science.gov (United States)

    Xiaojun Du; Qinfeng Guo; Xianming Gao; Keping Na

    2007-01-01

    Understanding the seed rain and seed loss dynamics in the natural condition has important significance for revealing the natural regeneration mechanisms.We conducted a 3-year field observation on seed rain, seed loss and natural regeneration of Castanopsis fargesii Franch., a dominant tree species in evergreen broad-leaved forests in Dujiangyan,...

  4. [Study on influence factors of seed germination and seeding growth of Lonicera macranthoides].

    Science.gov (United States)

    Xu, Jin; Zhang, Ying; Cui, Guang-Lin; She, Yue-Hui; Li, Long-Yun

    2016-01-01

    In order to improve reproductive efficiency and quality standard, the influence factors of seed germination and seeding growth of Lonicera macranthoides werew studied. The fruit and seed morphological characteristics of L. macranthoides were observed, the seed water absorbing capacity was determined, and different wet sand stratification time, temperature and germination bed treatment were set up. The effects of the parameters on seed germination and seedling growth were analysed. There was no obstacles of water absorption on L. macranthoides seed, quantity for 22 h water absorption was close to saturation. In the first 80 d, with the increase of the stratification time, seed initial germination time was shortened, germination rate and germination potential was improved. Stratification for 100 d, germination rate decreased. At 15 ℃, seed germination and seedling growth indicators were the best. The seedling cotyledon width in light was significantly higher than that in dark. Seeds on the top of paper and top of sand germination rate, germination potential, and germination index was significantly higher than that of other germination bed and mildew rate is low. The optimal conditions of seeds germination test was stratified in 4 ℃ wet sand for 80 d, 15 ℃ illuminate culture on the top of paper or top of sand. The first seeding counting time was the 4th day after beginning the test, the final time was the 23th day. The germination potential statistical time was the 13th day after beginning the test. Copyright© by the Chinese Pharmaceutical Association.

  5. Seed predators exert selection on the subindividual variation of seed size.

    Science.gov (United States)

    Sobral, M; Guitián, J; Guitián, P; Larrinaga, A R

    2014-07-01

    Subindividual variation among repeated organs in plants constitutes an overlooked level of variation in phenotypic selection studies, despite being a major component of phenotypic variation. Animals that interact with plants could be selective agents on subindividual variation. This study examines selective pressures exerted during post-dispersal seed predation and germination on the subindividual variation of seed size in hawthorn (Crataegus monogyna). With a seed offering experiment and a germination test, we estimated phenotypic selection differentials for average and subindividual variation of seed size due to seed predation and germination. Seed size affects germination, growth rate and the probability of an individual seed of escaping predation. Longer seeds showed higher germination rates, but this did not result in significant selection on phenotypes of the maternal trees. On the other hand, seed predators avoided wider seeds, and by doing so exerted phenotypic selection on adult average and subindividual variation of seed size. The detected selection on subindividual variation suggests that the levels of phenotypic variation within individual plants may be, at least partly, the adaptive consequence of animal-mediated selection. © 2013 German Botanical Society and The Royal Botanical Society of the Netherlands.

  6. Seed Priming with Melatonin Effects on Seed Germination and Seedling Growth in Maize under Salinity Stress

    International Nuclear Information System (INIS)

    Jiang, X.; Li, H.; Song, X.

    2016-01-01

    The effects on seed germination and seedling growth in maize under salinity stress by seed priming with melatonin were investigated. Seeds of maize cultivar Nonghua101 were soaked in 0.4, 0.8 and 1.6 mM aerated solution of melatonin for 24 h, and primed seeds were germinated under the condition of 150 mM NaCl with paper media. The results showed seed priming with 0.8 mM melatonin was the best performance of all the treatments to seed germination and seedling growth in maize under salinity stress. Then primed with 0.8 mM melatonin or water for 24 h and unprimed seeds were germination under the condition of 150 mM NaCl with sand media. The results showed seed priming with 0.8 mM melatonin significantly improved germination energy, germination percentage, seedling vigor index, shoot and root lengths, seedling fresh and dry weights, K/sup +/ content, relative water content, proline and total phenolic contents, superoxide dismutase, catalase and phenylalanin ammonia lyase activities; and significantly decreased mean emergence time, Na/sup +/ content, electrolyte leakage and malondialdehyde content compared with untreated seeds under salinity stress. These results suggest that seed priming with melatonin alleviates the salinity damage to maize and seed priming with melatonin may be an important alternative approach to decrease the impact of salinity stress in maize. (author)

  7. A Methodological Approach for Testing the Viability of Seeds Stored in Short-Term Seed Banks

    Directory of Open Access Journals (Sweden)

    Jose A. FORTE GIL

    2017-12-01

    Full Text Available Efficient management of ‘active’ seed banks – specifically aimed at the short-term storage at room temperature of seeds to be used locally in conservation/regeneration programmes of endemic or endangered plant species – requires establishing the optimal storage time to maintain high seed viability, for each stored species. In this work, germination of seeds of the halophytes Thalictrum maritimum, Centaurea dracunculifolia and Linum maritimum has been investigated. The seeds had been stored for different periods of time in the seed bank of ‘La Albufera’ Natural Park (Valencia, SE Spain after collection in salt marshes of the Park, where small populations of the three species are present. Seeds of T. maritimum and C. dracunculifolia have a relatively short period of viability at room temperature, and should not be stored for more than three years. On the other hand, L. maritimum seeds maintain a high germination percentage and can be kept at room temperature for up to 10 years. T. maritimum seeds, in contrast to those of the other two species, did not germinate in in vitro tests nor when sown directly on a standard substrate, unless a pre-treatment of the seeds was applied, mechanical scarification being the most effective. These results will help to improve the management of the seed bank, to generate more efficiently new plants for reintroduction and reinforcement of populations of these species in their natural ecosystems within the Natural Park.

  8. Biochemical changes during aging of soybean seed

    Directory of Open Access Journals (Sweden)

    Balešević-Tubić Svetlana

    2009-01-01

    Full Text Available Biochemical changes that occur in the seed as a result of ageing are very significant for seed quality and longevity. Because of its characteristic composition, processes occurring in the seed of oil crops during storage will be typical as well. Six soybean varieties developed in Institute of field and vegetable crops Novi Sad, submitted to accelerated and natural aging, under controlled and conventional storage conditions were used in these trials. The content of malondialdehyde, superoxide dismutase and peroxidase activities were studied. The biochemical processes i.e. lipid peroxidation, as well as the decrease in supeoxide dismutase and peroxidase activities (especially pronounced by applied accelerated aging were caused by both type of aging. The degree of seed damage and the ability of seed to resist the negative consequences of aging were influenced, beside duration of aging period, by type of storage and characteristics of soybean varieties. .

  9. Asymbiotic in vitro seed propagation of Dendrobium.

    Science.gov (United States)

    Teixeira da Silva, Jaime A; Tsavkelova, Elena A; Ng, Tzi Bun; Parthibhan, S; Dobránszki, Judit; Cardoso, Jean Carlos; Rao, M V; Zeng, Songjun

    2015-10-01

    The ability to germinate orchids from seeds in vitro presents a useful and viable method for the propagation of valuable germplasm, maintaining the genetic heterogeneity inherent in seeds. Given the ornamental and medicinal importance of many species within the genus Dendrobium, this review explores in vitro techniques for their asymbiotic seed germination. The influence of abiotic factors (such as temperature and light), methods of sterilization, composition of basal media, and supplementation with organic additives and plant growth regulators are discussed in context to achieve successful seed germination, protocorm formation, and further seedling growth and development. This review provides both a basis for the selection of optimal conditions, and a platform for the discovery of better ones, that would allow the development of new protocols and the exploration of new hypotheses for germination and conservation of Dendrobium seeds and seedlings.

  10. Biohydrogenation of Fatty Acids Is Dependent on Plant Species and Feeding Regimen of Dairy Cows

    DEFF Research Database (Denmark)

    Petersen, Majbritt Bonefeld; Jensen, Søren Krogh

    2014-01-01

    and LA between single plant species and feeding regimens. Rumen fluid was collected from cows fed either total mixed ration (TMR), species-rich silage (HERB), or grass silage (GRASS). Five single species (alfalfa, birdsfoot trefoil, chicory, English plantain, and salad burnet) and a grass–clover mixture...... (white clover and ryegrass) were incubated in three replicas up to 30 h and subsequently analyzed for fatty acid content. Michaelis–Menten kinetics was applied for quantifying the BH rate. BH proceeded at the lowest rate in alfalfa and salad burnet (P ... was lower in HERB and GRASS compared to TMR (P

  11. Feed intake and milk production in dairy cows fed different grass and legume species: a meta-analysis

    DEFF Research Database (Denmark)

    Johansen, Marianne; Lund, Peter; Weisbjerg, Martin Riis

    2018-01-01

    The aim of this meta-analysis was to compare feed intake, milk production, milk composition and organic matter (OM) digestibility in dairy cows fed different grass and legume species. Data from the literature was collected and different data sets were made to compare families (grasses v. legumes...... tannins in birdsfoot trefoil. None of the included grass species differed in DMI, milk production, milk composition or OM digestibility, indicating that different grass species have the same value for milk production, if OM digestibility is comparable. However, the comparison of different grass species...

  12. Changes in nutritive value and herbage yield during extended growth intervals in grass-legume mixtures

    DEFF Research Database (Denmark)

    Elgersma, Anjo; Søegaard, Karen

    2018-01-01

    . Perennial ryegrass was sown with each of four legumes: red clover, white clover, lucerne and birdsfoot trefoil, and white clover was sown with hybrid ryegrass, meadow fescue and timothy. Effects of species composition on herbage yield, contents of N, neutral detergent fibre (NDF), acid detergent fibre (ADF...... in quality parameters differed among species and functional groups, i.e., grasses and legumes. Results are discussed in the context of quantifying the impact of delaying the harvest date of grass–legume mixtures and relationships between productivity and components of feed quality....

  13. Seed yield components and their potential interaction in grasses - to what extend does seed weigth influence yield?

    DEFF Research Database (Denmark)

    Boelt, B; Gislum, R

    2010-01-01

     In a first-year seed crop of red fescue (Festuca rubra L.) the degree of lodging was controlled by the use of Moddus (Trinexapac-ethyl). Seed weight was found to increase by the decreasing degree of lodging prior to harvest. The higher seed weights were accompanied by higher yields even though...... the number of reproductive tillers and floret site utilization (FSU) were unaffected by the treatments. Seed yield is affected by several yield components and reflects the interaction between the seed yield potential (e.g. number of reproductive tillers, number of spikelets and florets/spikelet per...... reproductive tiller), the utilization of the potential (e.g. seed set, seed weight) and the realization of the seed yield potential, defined as the number of florets forming a saleable seed. The realization of the seed yield potential is affected by seed retention, seed weight and other traits associated...

  14. Influence the Rubber Seed Type and Altitude on Characteristic of Seed, Oil and Biodiesel

    Directory of Open Access Journals (Sweden)

    Salni Salni

    2017-06-01

    Full Text Available This research studies the influence of the type of rubber seed that is superior and local, altitude plant in South Sumatra province to the characteristic of seed, oil and biodiesel (methyl ester. Rubber plants planted from local rubber seed by seeds seedlings and superior rubber seed by selected clones. In the study, rubber plants planted at a different altitude, namely in Banyuasin district (18 m above sea level, Prabumulih District (176 m above sea level and Lahat District (627 m above sea level. The results showed that the weight of the flour, the water content and ash content in the local rubber seeds larger than the superior rubber seed for all altitude, but oil content a large in the superior rubber seeds. The major of fatty acids in the rubber seed oil in all types and altitude are a linoleic acid with a different percentage except local rubber seed oil from Lahat district with the large percentage of octadecanoic acid. Free fatty acids in the oil from the superior seeds rubber of 13.897-15.494 % large than local rubber seed oil was found 9.786-10.399 % for all altitude. By esterification process using sulfuric acid catalyst, Free Fatty Acid (FFA can be reduced to ≤ 2 %. The methyl ester made from the transesterification process of rubber seed oil after esterification using methanol and sodium hydroxide as catalyst. Analysis of methyl esters includes cetane index, flash point, kinematic viscosity, carbon residue, density, moisture content, water and sediment content and distillation compared with SNI 7182 and ASTM 6751-02.  The result indicated that the quality of methyl ester from superior rubber seed oil in the Banyuasin and Prabumulih district better than another methyl ester. The types of rubber seed altitude affect the characteristics of the seed, oil and methyl ester  but the altitude are not significantly different. Keywords: rubber seed, type, altitude, oil, biodiesel Article History: Received March 21st 2017; Received in

  15. Responses of Seed Germination, Seedling Growth, and Seed Yield Traits to Seed Pretreatment in Maize (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Yu Tian

    2014-01-01

    Full Text Available A series of seed priming experiments were conducted to test the effects of different pretreatment methods to seed germination, seedling growth, and seed yield traits in maize (Zea mays L.. Results indicated that the seeds primed by gibberellins (GA, NaCl, and polyethylene glycol (PEG reagents showed a higher imbibitions rate compared to those primed with water. The final germination percentage and germination rate varied with different reagents significantly (P<0.05. The recommended prime reagents were GA at 10 mg/L, NaCl at 50 mM, and PEG at 15% on account of germination experiment. 15% PEG priming reagent increased shoot and root biomass of maize seedling. The shoot biomass of seedlings after presoaking the seeds with NaCl reagent was significantly higher than the seedlings without priming treatment. No significant differences of plant height, leaf number, and hundred-grain weight were observed between control group and priming treatments. Presoaking with water, NaCl (50 mM, or PEG (15% significantly increased the hundred-grain weight of maize. Therefore, seed pretreatment is proved to be an effective technique to improve the germination performance, seedling growth, and seed yield of maize. However, when compared with the two methods, if immediate sowing is possible, presoaking is recommended to harvest better benefits compared to priming method.

  16. Seeds of hope, seeds of despair: towards a political economy of the seed industry in southern Africa.

    Science.gov (United States)

    Zerbe, N

    2001-01-01

    The seed industry in Southern Africa has been radically transformed by a policy of liberalisation and privatisation started under structural adjustment. Traditionally under the domain of parastatals, seed research, production and distribution has been criticised for failing to provide modern variety seed to smallholder farmers. However, the private companies which have stepped in to replace seed parastatals in southern Africa have proven no more effective in meeting the demands of smallholders. The Trade Related Intellectual Property Rights (TRIPs) Agreement, concluded in 1994 as part of the Uruguay Rounds of GATT negotiations, as well as certain biotechnological innovations such as Terminator or Traitor technologies, threaten to further undermine local seed production and consumption by destroying the informal seed sector so central to agricultural production in the region. What alternatives exist? The success of Zimbabwe's maize seed network offers some insight. Resting on a unique relationship between government and nationally based producer co-operatives, Zimbabwe's maize programme was able to provide nearly every farmer in the country with hybrid maize suited for local growing conditions.

  17. Responses of seed germination, seedling growth, and seed yield traits to seed pretreatment in maize (Zea mays L.).

    Science.gov (United States)

    Tian, Yu; Guan, Bo; Zhou, Daowei; Yu, Junbao; Li, Guangdi; Lou, Yujie

    2014-01-01

    A series of seed priming experiments were conducted to test the effects of different pretreatment methods to seed germination, seedling growth, and seed yield traits in maize (Zea mays L.). Results indicated that the seeds primed by gibberellins (GA), NaCl, and polyethylene glycol (PEG) reagents showed a higher imbibitions rate compared to those primed with water. The final germination percentage and germination rate varied with different reagents significantly (P germination experiment. 15% PEG priming reagent increased shoot and root biomass of maize seedling. The shoot biomass of seedlings after presoaking the seeds with NaCl reagent was significantly higher than the seedlings without priming treatment. No significant differences of plant height, leaf number, and hundred-grain weight were observed between control group and priming treatments. Presoaking with water, NaCl (50 mM), or PEG (15%) significantly increased the hundred-grain weight of maize. Therefore, seed pretreatment is proved to be an effective technique to improve the germination performance, seedling growth, and seed yield of maize. However, when compared with the two methods, if immediate sowing is possible, presoaking is recommended to harvest better benefits compared to priming method.

  18. Comparison of germination and seed bank dynamics of dimorphic seeds of the cold desert halophyte Suaeda corniculata subsp. mongolica

    Science.gov (United States)

    Cao, Dechang; Baskin, Carol C.; Baskin, Jerry M.; Yang, Fan; Huang, Zhenying

    2012-01-01

    Background and Aims Differences in dormancy and germination requirements have been documented in heteromorphic seeds of many species, but it is unknown how this difference contributes to maintenance and regeneration of populations. The primary aim of this study was to compare the seed bank dynamics, including dormancy cycling, of the two seed morphs (black and brown) of the cold desert halophyte Suaeda corniculata and, if differences were found, to determine their influence on regeneration of the species. Method Seeds of the two seed morphs were buried, exhumed and tested monthly for 24 months over a range of temperatures and salinities, and germination recovery and viability were determined after exposure to salinity and water stress. Seedling emergence and dynamics of the soil seed bank were also investigated for the two morphs. Key Results Black seeds had an annual dormancy/non-dormancy cycle, while brown seeds, which were non-dormant at maturity, remained non-dormant. Black seeds also exhibited an annual cycle in sensitivity of germination to salinity. Seedlings derived from black seeds emerged in July and August and those from brown seeds in May. Seedlings were recruited from 2·6 % of the black seeds and from 2·8 % of the brown seeds in the soil, and only 0·5 % and 0·4 % of the total number of black and brown seeds in the soil, respectively, gave rise to seedlings that survived to produce seeds. Salinity and water stress induced dormancy in black seeds and decreased viability of brown seeds. Brown seeds formed only a transient soil seed bank and black seeds a persistent seed bank. Conclusions The presence of a dormancy cycle in black but not in brown seeds of S. corniculata and differences in germination requirements of the two morphs cause them to differ in their germination dynamics. The study contributes to our limited knowledge of dormancy cycling and seed bank formation in species producing heteromorphic seeds. PMID:22975287

  19. Climate Change: Seed Production and Options for Adaptation

    Directory of Open Access Journals (Sweden)

    John G. Hampton

    2016-07-01

    Full Text Available Food security depends on seed security and the international seed industry must be able to continue to deliver the quantities of quality seed required for this purpose. Abiotic stress resulting from climate change, particularly elevated temperature and water stress, will reduce seed yield and quality. Options for the seed industry to adapt to climate change include moving sites for seed production, changing sowing date, and the development of cultivars with traits which allow them to adapt to climate change conditions. However, the ability of seed growers to make these changes is directly linked to the seed system. In the formal seed system operating in developed countries, implementation will be reasonably straight forward. In the informal system operating in developing countries, the current seed production challenges including supply failing to meet demand and poor seed quality will increase with changing climates.

  20. Seed coat development in Velloziaceae: primary homology assessment and insights on seed coat evolution.

    Science.gov (United States)

    Sousa-Baena, Mariane S; de Menezes, Nanuza L

    2014-09-01

    Seed coat characteristics have historically been used to infer taxonomic relationships and are a potential source of characters for phylogenetic reconstruction. In particular, seed coat morphoanatomy has never been studied in detail in Velloziaceae. One character based on seed surface microsculpture has been used in phylogenies, but was excluded from recent studies owing to problems in primary homology. This work aimed to clarify the origin and general composition of seed coat cell layers in Velloziaceae and to propose hypotheses of primary homology among seed characters.• Seed coat development of 24 Velloziaceae species, comprising nine genera, and one species of Pandanaceae (outgroup) was studied using standard anatomical methods. Developmental data were interpreted in the light of a recently published phylogeny.• Eight types of seed coat were identified. Whereas the most common type has four distinct cell layers (two-layered tegmen and testa), we encountered much more variation in seed coat composition than previously reported, the analysis of which revealed some potential synapomorphies. For instance, an exotesta with spiral thickenings may be a synapomorphy of Barbacenia.• Our results showed that the character states previously used in phylogenies are not based on homologous layers and that the same state was misattributed to species exhibiting quite different seed coats. This study is a first step toward a better understanding of seed coat structure evolution in Velloziaceae. © 2014 Botanical Society of America, Inc.

  1. Grazing impact on desert plants and soil seed banks: Implications for seed-eating animals

    Science.gov (United States)

    Pol, Rodrigo G.; Sagario, M. Cecilia; Marone, Luis

    2014-02-01

    We assess whether the knowledge of livestock diet helps to link grazing effects with changes in plant cover and soil seed bank size, aiming at inferring the consequences of grazing on seed-eating animals. Specifically, we test whether continuous and heavy grazing reduce the cover, number of reproductive structures and seed reserves of the same grass species whose seeds are selected and preferred by granivorous animals in the central Monte desert, Argentina. Grass cover and the number of grass spikes usually diminished under grazing conditions in the two localities studied (Telteca and Ñacuñán), and soil seed bank was consistently reduced in all three years evaluated owing to a decline of perennial grass and forb seeds. In particular, the abundance of those seeds selected and preferred by birds and ants (in all cases grass species) declined 70-92% in Ñacuñán, and 52-72% in Telteca. Reduction of perennial grass cover and spike number in grazed sites reinforced the causal link between livestock grazing and the decline of grass soil seed reserves throughout failed plant reproduction. Grass seed bank depletion suggests that grazing may trigger a "cascade" of mechanisms that affect the abundance and persistence of valuable fodder species as well as the availability of seed resources for granivorous animals.

  2. Seed-specific overexpression of AtFAX1 increases seed oil content in Arabidopsis.

    Science.gov (United States)

    Tian, Yinshuai; Lv, Xueyan; Xie, Guilan; Zhang, Jing; Xu, Ying; Chen, Fang

    2018-06-02

    Biosynthesis of plant seed oil is accomplished through the coordinate action of multiple enzymes in multiple subcellular compartments. Fatty acid (FA) has to be transported from plastid to endoplasmic reticulum (ER) for TAG synthesis. However, the role of plastid FA transportation during seed oil accumulation has not been evaluated. AtFAX1 (Arabidopsis fatty acid export1) mediated the FA export from plastid. In this study, we overexpressed AtFAX1 under the control of a seed specific promoter in Arabidopsis. The resultant overexpression lines (OEs) produced seeds which contained 21-33% more oil and 24-30% more protein per seed than those of the wild type (WT). The increased oil content was probably because of the enhanced FA and TAG synthetic activity. The seed size and weight were both increased accordingly. In addition, the seed number per silique and silique number per plant had no changes in transgenic plants. Taken together, our results demonstrated that seed specific overexpression of AtFAX1 could promote oil accumulation in Arabidopsis seeds and manipulating FA transportation is a feasible strategy for increasing the seed oil content. Copyright © 2018 Elsevier Inc. All rights reserved.

  3. Colonization of citrus seed coats by 'Candidatus Liberibacter asiaticus': implications for seed transmission of the bacterium.

    Science.gov (United States)

    Hilf, Mark E

    2011-10-01

    Huanglongbing is an economically damaging disease of citrus associated with infection by 'Candidatus Liberibacter asiaticus'. Transmission of the organism via infection of seeds has not been demonstrated but is a concern since some citrus varieties, particularly those used as rootstocks in commercial plantings are propagated from seed. We compared the incidence of detection of 'Ca. Liberibacter asiaticus' DNA in individual fruit peduncles, seed coats, seeds, and in germinated seedlings from 'Sanguenelli' sweet orange and 'Conners' grapefruit fruits sampled from infected trees. Using real-time quantitative PCR (qPCR) we detected pathogen DNA in nucleic acid extracts of 36 and 100% of peduncles from 'Sanguenelli' and from 'Conners' fruits, respectively. We also detected pathogen DNA in extracts of 37 and 98% of seed coats and in 1.6 and 4% of extracts from the corresponding seeds of 'Sanguenelli' and 'Conners', respectively. Small amounts of pathogen DNA were detected in 10% of 'Sanguenelli' seedlings grown in the greenhouse, but in none of 204 extracts from 'Conners' seedlings. Pathogen DNA was detected in 4.9% and in 89% of seed coats peeled from seeds of 'Sanguenelli' and 'Conners' which were germinated on agar, and in 5% of 'Sanguenelli' but in none of 164 'Conners' seedlings which grew from these seeds on agar. No pathogen DNA was detected in 'Ridge Pineapple' tissue at 3 months post-grafting onto 'Sanguenelli' seedlings, even when pathogen DNA had been detected initially in the 'Sanguenelli' seedling. Though the apparent colonization of 'Conners' seeds was more extensive and nearly uniform compared with 'Sanguenelli' seeds, no pathogen DNA was detected in 'Conners' seedlings grown from these seeds. For either variety, no association was established between the presence of pathogen DNA in fruit peduncles and seed coats and in seedlings.

  4. Ecological longevity of Polaskia chende (Cactaceae) seeds in the soil seed bank, seedling emergence and survival.

    Science.gov (United States)

    Ordoñez-Salanueva, C A; Orozco-Segovia, A; Canales-Martínez, M; Seal, C E; Pritchard, H W; Flores-Ortiz, C M

    2017-11-01

    Soil seed banks are essential elements of plant population dynamics, enabling species to maintain genetic variability, withstand periods of adversity and persist over time, including for cactus species. However knowledge of the soil seed bank in cacti is scanty. In this study, over a 5-year period we studied the seed bank dynamics, seedling emergence and nurse plant facilitation of Polaskia chende, an endemic columnar cactus of central Mexico. P. chende seeds were collected for a wild population in Puebla, Mexico. Freshly collected seeds were sown at 25 °C and 12-h photoperiod under white light, far-red light and darkness. The collected seeds were divided in two lots, the first was stored in the laboratory and the second was use to bury seeds in open areas and beneath a shrub canopy. Seeds were exhumed periodically over 5 years. At the same time seeds were sown in open areas and beneath shrub canopies; seedling emergence and survival were recorded over different periods of time for 5 years. The species forms long-term persistent soil seed banks. The timing of seedling emergence via germination in the field was regulated by interaction between light, temperature and soil moisture. Seeds entered secondary dormancy at specific times according to the expression of environmental factors, demonstrating irregular dormancy cycling. Seedling survival of P. chende was improved under Acacia constricta nurse plants. Finally, plant facilitation affected the soil seed bank dynamics as it promoted the formation of a soil seed bank, but not its persistence. © 2017 German Society for Plant Sciences and The Royal Botanical Society of the Netherlands.

  5. Effects of gamma radiation in annatto seeds

    International Nuclear Information System (INIS)

    Franco, Camilo F. de Oliveira; Arthur, Valter; Arthur, Paula B.; Harder, Marcia N.C.; Filho, Jose C.; Neto, Miguel B.

    2015-01-01

    The annatto bixin has emerged as a major source of natural dyes used in the world notably by the substitution of synthetics harmful to human health and ecologic tendency in obtaining industrial products free of additives with applications in industries textiles; cosmetics; pharmaceutical and food mainly. The aim of this research was to obtain increased of germination rate and dormancy breaking on annatto seeds by gamma radiation. Annatto dry seeds were exposed to low doses of gamma radiation from source of Cobalt-60, type Gammecell-220, at 0.456 kGy/hour dose rate. In order to study stimulation effects of radiation on germination rate and dormancy breaking in the seeds. Five treatments with gamma radiation doses were applied as follows: 0 (control); 100; 125; 150 and 175 Gy. After irradiation the annatto seeds were planted as for usual seed production. According to the results obtained in this experiment we can conclude that the low doses of gamma radiation utilized on the annatto seeds did not presented significantly effect on the germination of plants. But the best dose to increase the germination of seeds was 150 Gy. (author)

  6. Seed ecology of Bromus sterilis L.

    Directory of Open Access Journals (Sweden)

    Žďárková, Veronika

    2014-02-01

    Full Text Available Bromus sterilis L. (barren brome has become a troublesome weed of winter cereals in reduced tillage systems, mainly in South and North America, middle and Western Europe. In the Czech Republic, its importance has increased dramatically over the past 10 years. Barren brome is reported as a problem weed in other winter crops such as oil seed rape, in vineyards and in other cultivated places. In this study, the dormancy and germination under different temperatures, water and light regimes were investigated. Emergence from different depths and persistence in the soil profile were investigated under field conditions. The seeds of Bromus sterilis showed broad ecological valence to hydrothermal factors germinating in the wide range of 5 to 35 °C. Similarly, no strong effect on the germination in an environment with low water potential was observed. The response to light at various temperatures showed that seeds germinated better in darkness. The emergence declined significantly with burial depth (under 40 mm. The loss of primary dormancy was rapid in time and only 50% of the seeds germinated within 8 weeks after collecting from maternal plants. The seeds were not able to survive in the soil seed bank for a longer time and fall seeds lost viability 1 year after burial in a soil profile.

  7. Seed germination behavior of swallow wort

    Directory of Open Access Journals (Sweden)

    amir hosein pahlavani

    2009-06-01

    Full Text Available The exotic plant, Swallow- wort, a twining perennial of the Milkweed family, has become increasingly invasive in some place of Iran, especially orchards. Increased knowledge of wort germination biology would facilitate development of an optimum control program. Germination of Swallow wort seeds as affected by environmental factors was studied under controlled-environment growth chamber conditions. The following studies were conducted in plant Pests & Diseases Research Institute during the years 2003-4: 1- Effect of constant temperature on germination that including 10, 15, 18, 20, 25, 30, 35 and 40˚C; 2- Effect of light on constant germination; 3- Effect of temperature fluctuations on seed germination: 15/7, 20/12, 25/17 and 30/22˚C. All experiments were conducted with 8 replications. Swallow wort seeds showed no dormancy when detachment from mother plant. Seed germination was strongly influenced by temperature. Light did not play a crucial role on seed germination of this weed. Therefore Swallow wort seeds were not photoblastic and temperature fluctuations did not increase seed germination of Swallow wort. The above characteristics are very important in making swallowwort an invasive weed. Having precise information of these traits enables us to a better management and control of this troublesome weed.

  8. Oxygen requirement of germinating flax seeds

    Science.gov (United States)

    Kuznetsov, Oleg A.; Hasenstein, K. H.

    2003-05-01

    Plant experiments in earth orbit are typically prepared on the ground and germinated in orbit to study gravity effects on the developing seedlings. Germination requires the breakdown of storage compounds, and this metabolism depends upon respiration, making oxygen one of the limiting factors in seed germination. In microgravity lack of run-off of excess water requires careful testing of water dispensation and oxygen availability. In preparation for a shuttle experiment (MICRO on STS-107) we studied germination and growth of flax ( Linum usitatissimum L.) seedlings in the developed hardware (Magnetic Field Chamber, MFC). We tested between four to 32 seeds per chamber (air volume = 14 mL) and after 36 h measured the root length. At 90 μl O 2 per seed (32 seeds/chamber), the germination decreased from 94 to 69%, and the root length was reduced by 20%, compared to 8 seeds per chamber. Based on the percent germination and root length obtained in controlled gas mixtures between 3.6 and 21.6% O 2 we determined the lower limit of reliable germination to be 10 vol. % O 2 at atmospheric pressure. Although the oxygen available in the MFC's can support the intended number of seeds, the data show that seed storage and microgravity-related limitations may reduce germination.

  9. Oxygen requirement of germinating flax seeds

    Science.gov (United States)

    Kuznetsov, Oleg A.; Hasenstein, K. H.; Hasentein, K. H. (Principal Investigator)

    2003-01-01

    Plant experiments in earth orbit are typically prepared on the ground and germinated in orbit to study gravity effects on the developing seedlings. Germination requires the breakdown of storage compounds, and this metabolism depends upon respiration, making oxygen one of the limiting factors in seed germination. In microgravity lack of run-off of excess water requires careful testing of water dispensation and oxygen availability. In preparation for a shuttle experiment (MICRO on STS-107) we studied germination and growth of flax (Linum usitatissimum L.) seedlings in the developed hardware (Magnetic Field Chamber, MFC). We tested between four to 32 seeds per chamber (air volume=14 mL) and after 36 h measured the root length. At 90 microliters O2 per seed (32 seeds/chamber), the germination decreased from 94 to 69%, and the root length was reduced by 20%, compared to 8 seeds per chamber. Based on the percent germination and root length obtained in controlled gas mixtures between 3.6 and 21.6% O2 we determined the lower limit of reliable germination to be 10 vol. % O2 at atmospheric pressure. Although the oxygen available in the MFC's can support the intended number of seeds, the data show that seed storage and microgravity-related limitations may reduce germination. c2003 Published by Elsevier Ltd on behalf of COSPAR.

  10. Sowing seasons and quality of soybean seeds

    Directory of Open Access Journals (Sweden)

    Ávila Marizangela Rizzatti

    2003-01-01

    Full Text Available Considering the difficulties of producing high quality soybean [Glycine max (L. Merrill] seeds during the traditional cropping period in some areas of the State of Paraná, Brazil, a research project was carried out with the objective of evaluating the influence of sowing dates on the physiological and sanitary quality of seeds, during the 1998/99 and 1999/00 cropping seasons, in Maringá, PR, Brazil. The experiment consisted of five cultivar competition assays, arranged in a completely randomized block design, with each assay sown at different dates (10/15, 10/30, 11/15, 11/30 and 12/15 for each cropping season. The evaluated cultivars were BRS 132 (early, BRS 133 (semi-early, BR 16 (semi-early, BRS 134 (intermediate and FT- Estrela (late. Seeds obtained at the sowing dates were evaluated in the laboratory by germination, accelerated aging, and health tests. Sowing in November resulted in seeds with superior physiological and health quality. Cultivar BRS 133 showed the greatest stability in seed production with better quality for the different sowing dates. Cultivars BRS 134 and BRS 133, which were sown during the period from 10/15 to 11/30, produced seeds that had higher percentages of normal seedlings in the germination and accelerated aging tests. Advancing or delaying sowing dates had adverse effects on soybean seed production with regard to their sanitary quality.

  11. Effects of gamma radiation in annatto seeds

    Energy Technology Data Exchange (ETDEWEB)

    Franco, Camilo F. de Oliveira, E-mail: camilo.urucum@hotmail.com [Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA/EMEPA), Joao Pessoa, PB (Brazil); Arthur, Valter; Arthur, Paula B., E-mail: arthur@cena.usp.br [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil); Harder, Marcia N.C., E-mail: marcia.harder@fatec.sp.gov.br [Centro Paula Souza, Curso Superior de Tecnologia em Bicombustiveis (FATEC), Piracicaba, SP (Brazil); Filho, Jose C.; Neto, Miguel B., E-mail: jorgecazefilho@yahoo.com.br [Empresa Estadual de Pesquisa Agropecuaria da Paraiba (EMEPA), Joao Pessoa, PB (Brazil)

    2015-07-01

    The annatto bixin has emerged as a major source of natural dyes used in the world notably by the substitution of synthetics harmful to human health and ecologic tendency in obtaining industrial products free of additives with applications in industries textiles; cosmetics; pharmaceutical and food mainly. The aim of this research was to obtain increased of germination rate and dormancy breaking on annatto seeds by gamma radiation. Annatto dry seeds were exposed to low doses of gamma radiation from source of Cobalt-60, type Gammecell-220, at 0.456 kGy/hour dose rate. In order to study stimulation effects of radiation on germination rate and dormancy breaking in the seeds. Five treatments with gamma radiation doses were applied as follows: 0 (control); 100; 125; 150 and 175 Gy. After irradiation the annatto seeds were planted as for usual seed production. According to the results obtained in this experiment we can conclude that the low doses of gamma radiation utilized on the annatto seeds did not presented significantly effect on the germination of plants. But the best dose to increase the germination of seeds was 150 Gy. (author)

  12. Seed shape quantification in the order Cucurbitales

    Directory of Open Access Journals (Sweden)

    Emilio Cervantes

    2018-02-01

    Full Text Available Seed shape quantification in diverse species of the families belonging to the order Cucurbitales is done based on the comparison of seed images with geometric figures. Quantification of seed shape is a useful tool in plant description for phenotypic characterization and taxonomic analysis. J index gives the percent of similarity of the image of a seed with a geometric figure and it is useful in taxonomy for the study of relationships between plant groups. Geometric figures used as models in the Cucurbitales are the ovoid, two ellipses with different x/y ratios and the outline of the Fibonacci spiral. The images of seeds have been compared with these figures and values of J index obtained. The results obtained for 29 species in the family Cucurbitaceae support a relationship between seed shape and species ecology. Simple seed shape, with images resembling simple geometric figures like the ovoid, ellipse or the Fibonacci spiral, may be a feature in the basal clades of taxonomic groups.

  13. Nutritional evaluation of dried tomato seeds.

    Science.gov (United States)

    Persia, M E; Parsons, C M; Schang, M; Azcona, J

    2003-01-01

    Two samples of tomato seeds, a by-product of the tomato canning industry were evaluated to determine proximate analysis, amino acid content, and digestibility, TMEn, and protein efficiency ratio. Tomato seeds were also used to replace corn and soybean meal (SBM) in a chick diet on an equal true amino acid digestibility and TMEn basis. Tomato seeds were found to contain 8.5% moisture, 25% CP, 20.0% fat, 3.1% ash, 35.1% total dietary fiber, 0.12% Ca, 0.58% P, and 3,204 kcal/kg of TMEn. The total amounts of methionine, cystine, and lysine in the tomato seeds were 0.39, 0.40, and 1.34%, respectively, and their true digestibility coefficients, determined in cecectomized roosters, were 75, 70, and 54%, respectively. The protein efficiency ratio (weight gain per unit of protein intake) value when fed to chicks at 9% CP was 2.5 compared to 3.6 for SBM (P seeds could replace corn and SBM without any adverse affects on chick weight gain, feed intake, or gain:feed ratio from 8 to 21 d posthatch. Tomato seeds at any level in the diet did not significantly affect skin pigmentation. Although the protein quality of tomato seeds may not be as high as SBM, tomato seeds do contain substantial amounts of digestible amino acids and TMEn. When formulating diets on a true digestible amino acid and TMEn basis, tomato seeds can be supplemented into chick rations at up to 15% without any adverse affects on growth performance.

  14. The oxygen requirement of germinating flax seeds

    Science.gov (United States)

    Kuznetsov, O.; Hasenstein, K.

    Experiments for earth orbit are typically prepared on the ground and often germinated in orbit in order to study gravity effects on developing seedlings. Germination requires the breakdown of storage compounds and respiration. In orbit the formation of a water layer around the seed may further limit oxygen availability. Therefore, the oxygen content of the available gas volume is one of the limiting factors for seed germination. In preparation for an upcoming shuttle experiment (MICRO on STS-107) we studied germination and growth of flax (Linum usitatissimum L.) seedlings in the developed hardware. We tested per seed chamber (gas volume = 14 mL, O2 = 2.9 mL) between 4 to 32 seeds glued to germination paper by 1% (w/v) gum guar. A lexan cover and a gasket hermetically sealed each of the eight chambers. For imbibition of the seeds a previously optimized amount of distilled water was dispensed through sealed inlets. The seedlings were allowed to grow for either 32 to 48 h on a clinostat or without microgravity simulation. Then their root length was measured. With 32 seeds per chamber, four times the intended number of seeds for the flight, the germination rate decreased from 94 to 69%, and the root length was reduced by 20%. Experiments on the germination and root length in controlled atmospheres (5, 10, 15 and 21% O2 ) suggest that germination and growth for two days requires about 200 :l of O (1 mL air) per seed. Our2 experiments correlate oxygen dependency from seed mass and germination temperature, and analyze accumulation of gaseous metabolites (supported by NASA grant NAG10-0190).

  15. EPR in characterization of seeds paramagnetic species

    International Nuclear Information System (INIS)

    Luiz, A.P.C.; Mauro, M.F.F.L.; Portugal, K.O.; Barbana, V.M.; Guedes, C.L.B.; Mauro, E. di; Carneiro, C.E.A.; Zaia, D.A.M.; Prete, C.E.C.

    2011-01-01

    Full text. In Brazil, since 1970s, renewable fuel programs has been developed in order to replace petroleum. Today a program that has been discussed is the bio diesel, which intend to replace diesel fuel, fossil oil, to bio diesel, renewal fuel. As seeds are the basis for production of oil and consequently processed into bio diesel, the goal of this work is to characterize and compare paramagnetic species present in the seeds by Electron Paramagnetic Resonance (EPR). Samples used in this study were seeds of sorghum, barley, corn, peanuts, soy beans, cotton, wheat, oats, mustard, rice, sunflower and turnip. Some paramagnetic species present in soil was also investigated as goethite (FeOOH), hematite (Fe 2 O 3 ), magnetite (Fe 3 O 4 ), and ferrihydrite (Fe 5 HO 8 · 4H 2 O), since, these species present in appreciable quantities in the soil can be present in the seeds and analyzed for comparison. The characterization of these species is essential to understand the EPR seeds spectra. Each sample is placed in a thin quartz tube 4 mm in diameter, and it is inserted into the cavity of the spectrometer at room temperature, at low temperature (77 K) and variable temperature using liquid nitrogen flow and hot flow through a compressor air. It was used as standard Mg O:Mn 2+ , which is also inserted into the cavity. Shortly after the potency is regulated, frequency, amplitude and sweep the field. The spectroscopic analysis by EPR X-band (∼ 9:5GHz), were performed at the Fluorescence and Electron Paramagnetic Resonance Laboratory, Exact Sciences Center, State University of Londrina, Parana state, Brazil, through an EPR spectrometer JEOL brand (JES-PE-3X). In the EPR spectra, spectroscopic factor or g factor and line width were determined in paramagnetic species. Studies from several seeds with EPR technique detected in all of them presence of same complex of Fe 3+ present in the goethite at g ∼ 2, and in the seeds exist free radicals at g = 2:004, at room temperature

  16. EPR in characterization of seeds paramagnetic species

    Energy Technology Data Exchange (ETDEWEB)

    Luiz, A.P.C.; Mauro, M.F.F.L.; Portugal, K.O.; Barbana, V.M.; Guedes, C.L.B.; Mauro, E. di; Carneiro, C.E.A.; Zaia, D.A.M.; Prete, C.E.C. [Universidade Estadual de Londrina (UEL), PR (Brazil)

    2011-07-01

    Full text. In Brazil, since 1970s, renewable fuel programs has been developed in order to replace petroleum. Today a program that has been discussed is the bio diesel, which intend to replace diesel fuel, fossil oil, to bio diesel, renewal fuel. As seeds are the basis for production of oil and consequently processed into bio diesel, the goal of this work is to characterize and compare paramagnetic species present in the seeds by Electron Paramagnetic Resonance (EPR). Samples used in this study were seeds of sorghum, barley, corn, peanuts, soy beans, cotton, wheat, oats, mustard, rice, sunflower and turnip. Some paramagnetic species present in soil was also investigated as goethite (FeOOH), hematite (Fe{sub 2}O{sub 3}), magnetite (Fe{sub 3}O{sub 4}), and ferrihydrite (Fe{sub 5}HO{sub 8} {center_dot} 4H{sub 2}O), since, these species present in appreciable quantities in the soil can be present in the seeds and analyzed for comparison. The characterization of these species is essential to understand the EPR seeds spectra. Each sample is placed in a thin quartz tube 4 mm in diameter, and it is inserted into the cavity of the spectrometer at room temperature, at low temperature (77 K) and variable temperature using liquid nitrogen flow and hot flow through a compressor air. It was used as standard Mg O:Mn{sup 2+}, which is also inserted into the cavity. Shortly after the potency is regulated, frequency, amplitude and sweep the field. The spectroscopic analysis by EPR X-band ({approx} 9:5GHz), were performed at the Fluorescence and Electron Paramagnetic Resonance Laboratory, Exact Sciences Center, State University of Londrina, Parana state, Brazil, through an EPR spectrometer JEOL brand (JES-PE-3X). In the EPR spectra, spectroscopic factor or g factor and line width were determined in paramagnetic species. Studies from several seeds with EPR technique detected in all of them presence of same complex of Fe{sup 3+} present in the goethite at g {approx} 2, and in the seeds

  17. INFLUENCE OF TREATMENT ON MAIZE SEED QUALITY

    Directory of Open Access Journals (Sweden)

    Ivica Beraković

    2012-12-01

    Full Text Available Due to the increasing occurrence of major pests ON corn, hybrid seed is necessary to be protected against pests and seed corn should be treated with appropriately insecticides. Choosing better technological solutions and choosing and appropriate insecticide seed treatment for corn can significantly reduce pest attack and thus enhance the production of corn. The aim of this research was to obtain based upon result information on the impact of treatment on quality of maize seed as well as the means to improve the conditions of storage and preservation of semen quality. Investigation and checking if insecticide treated seed adverse phytotoxic effect on plant growth and development in field conditions. The results indicate a significant effect of insecticide seed treatments on germination energy and non standard germination. A very significant influence of treated seed storage was also found on quality seeds. Looking at the impact of the treated hybrid on germination vigor and standard germination, a very significant hybrid impact was found out. The highest quality of the seed semen during the study was noticed with the seed of hybrid “H2”, followed by hybrid “H1”, while the hybrids “H3” and “H4” possessed less. The research shows that hybrids “H2” and “H1” are more suitable for seed treatment with insecticides than “H3” and “H4” hybrids. The field observations and research results obtained indicate a very significant impact of the treated seed on the above ground parts of plants, above ground mass, stem thickness and a very significant impact on plant spacing. The positive effect of treatment with “T1” and “T3” insecticides is visible in all conducted field researches. Plants treated with common fungicides and insecticides “T1” and “T3” had a greater height of the above ground plant parts, a larger mass of the above ground stems, greater stem thickness and better plant density treatments, compared to

  18. Pre-sowing irradiation of vegetable seeds

    Energy Technology Data Exchange (ETDEWEB)

    Stoyanov, S

    1975-01-01

    Results are reported of trials with radiation stimulation of vegetable crops conducted under farm conditions in different regions. They were based on exact tests accomplished at the IGHB. Pre-sowing irradiation of seed increased the greenhouse yield of Extase tomato variety by 15%; it increased the yield of Triumph tomato variety under field conditions as well. The pepper variety Kourtovska Kapiya and eggplant variety Bulgarski 12, both grown from irradiated seed in open field produced 18% and 5.5% higher yields, respectively. Irradiation of vegetable seeds finds a large application in the farm practice.

  19. Nutrient value of leaf versus seed

    Directory of Open Access Journals (Sweden)

    Marvin Edelman

    2016-07-01

    Full Text Available Major differences stand out between edible leaves and seeds in protein quality, vitamin and mineral concentrations and omega 6 / omega 3 fatty acid ratios. Data for seeds (wheat, rice, corn, soy, lentil, chick pea are compared with corresponding data for edible green leaves (kale, spinach, broccoli, duckweed. An x/y representation of data for lysine and methionine content highlights the group differences between grains, pulses, leafy vegetables and animal foods. Leaves come out with flying colors in all these comparisons. The perspective ends with a discussion on So why do we eat mainly seeds?

  20. Freezing tolerance of conifer seeds and germinants.

    Science.gov (United States)

    Hawkins, B J; Guest, H J; Kolotelo, D

    2003-12-01

    Survival after freezing was measured for seeds and germinants of four seedlots each of interior spruce (Picea glauca x engelmannii complex), lodgepole pine (Pinus contorta Dougl. ex Loud.), Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and western red cedar (Thuja plicata Donn ex D. Donn). Effects of eight seed treatments on post-freezing survival of seeds and germinants were tested: dry, imbibed and stratified seed, and seed placed in a growth chamber for 2, 5, 10, 15, 20 or 30 days in a 16-h photoperiod and a 22/17 degrees C thermoperiod. Survival was related to the water content of seeds and germinants, germination rate and seedlot origin. After freezing for 3 h at -196 degrees C, dry seed of most seedlots of interior spruce, Douglas-fir and western red cedar had 84-96% germination, whereas lodgepole pine seedlots had 53-82% germination. Freezing tolerance declined significantly after imbibition in lodgepole pine, Douglas-fir and interior spruce seed (western red cedar was not tested), and mean LT50 of imbibed seed of these species was -30, -24.5 and -20 degrees C, respectively. Freezing tolerance continued to decline to a minimum LT50 of -4 to -7 degrees C after 10 days in a growth chamber for interior spruce, Douglas-fir and lodgepole pine, or after 15 days for western red cedar. Minimum freezing tolerance was reached at the stage of rapid hypocotyl elongation. In all species, a slight increase in freezing tolerance of germinants was observed once cotyledons emerged from the seed coat. The decrease in freezing tolerance during the transition from dry to germinating seed correlated with increases in seed water content. Changes in freezing tolerance between 10 and 30 days in the growth chamber were not correlated with seedling water content. Within a species, seedlots differed significantly in freezing tolerance after 2 or 5 days in the growth chamber. Because all seedlots of interior spruce and lodgepole pine germinated quickly, there was no correlation

  1. Pre-sowing irradiation of vegetable seeds

    International Nuclear Information System (INIS)

    Stoyanov, S.

    1975-01-01

    Results are reported of trials with radiation stimulation of vegetable crops conducted under farm conditions in different regions. They were based on exact tests accomplished at the IGHB. Pre-sowing irradiation of seed increased the greenhouse yield of Extase tomato variety by 15%; it increased the yield of Triumph tomato variety under field conditions as well. The pepper variety Kourtovska Kapiya and eggplant variety Bulgarski 12, both grown from irradiated seed in open field produced 18% and 5.5% higher yields, respectively. Irradiation of vegetable seeds finds a large application in the farm practice. (author)

  2. Electrophoretic studies on rape seed proteins

    International Nuclear Information System (INIS)

    Chaudry, M.A.; Starr, A.; Bibi, N.

    1992-07-01

    Electrophoresis is a technique which separates biological molecules on the basis of charge and mass properties. The technique is used for separation, purification, characterization and identification of molecules/ compounds. Two major objectives for applications of electrophoresis have been studied in this report i.e. characterization of rape seed proteins and enzymes and identification of rape seed cultivars by polyacrylamide gel electrophoresis (PAGE). Gamma irradiation is being successfully used to create genetic variability and germination which brought about definite changes in the rape seed proteins reflected in different bands. These differences could be used to study variability in crop plants. (A.B.)

  3. Barley seed proteomics from spots to structures

    DEFF Research Database (Denmark)

    Finnie, Christine; Svensson, Birte

    2009-01-01

    forms on 2D-gels. Specific protein families, including peroxidases and alpha-amylases have been subjected to in-depth analysis resulting in characterisation of different isozymes, post-translational. modifications and processing. A functional proteomics study focusing on the seed thioredoxin system has...... with information from rice and other cereals facilitate identification of barley proteins. Several hundred barley seed proteins are identified and lower abundance proteins including membrane proteins are now being analysed. In the present review we focus on variation in protein profiles of seed tissues during...

  4. Gender inequality in predispersal seed predation contributes to female seed set advantage in a gynodioecious species.

    Science.gov (United States)

    Clarke, Gretel L; Brody, Alison K

    2015-05-01

    Most flowering plants are hermaphrodites. However, in gynodioecious species, some members of the population are male-sterile and reproduce only by setting seed, while others gain fitness through both male and female function. How females compensate for the loss of male function remains unresolved for most gynodioecious species. Here, as with many plants, fitness differences may be influenced by interactions with multiple species. However, whether multiple species interactions result in gender-specific fitness differences remains unknown. Using observational data from 2009-2010, we quantified seed set of the two sex morphs of Polemonium foliosissimu and asked how it is affected by pollination, and seed predation from a dipteran predispersal seed predator (Anthomyiidae: Hylemya sp.). We assessed seed production and losses to predation in 27 populations for one year and in six populations for a second year. Females set significantly more seed than did hermaphrodites in both years. Of the fitness components we assessed, including the number of flowers per plant, fruit set, seeds/fruit, and proportion of fruits destroyed by Hylemya, only fruit destruction differed significantly between the sexes. In one year, seeds/fruit and predation had a stronger effect on seed set for hermaphrodites than for females. Because predispersal seed predators do not pollinate flowers, their effects may depend on successful pollination of flowers on which they oviposit. To examine if genders differed in pollen limitation and seed predation and/or their interactive effects, in 2011 we hand-pollinated flowers and removed seed predator eggs in a fully factorial design. Both sexes were pollen limited, but their degree of pollen limitation did not differ. However, predation reduced.seed set more for hermaphrodites than for females. We found no significant interaction between hand pollen and seed predation, and no interaction between hand pollination and gender. Our results suggest that while

  5. Influence of Rice Seeding Rate on Efficacies of Neonicotinoid and Anthranilic Diamide Seed Treatments against Rice Water Weevil

    Directory of Open Access Journals (Sweden)

    Jason Hamm

    2014-12-01

    Full Text Available Rice in the U.S. is frequently seeded at low rates and treated before sowing with neonicotinoid or anthranilic diamide insecticides to target the rice water weevil. A previous study of the influence of seeding rate on rice water weevil densities showed an inverse relationship between seeding rates and immature weevil densities. This study investigated interactive effects of seeding rate and seed treatment on weevil densities and rice yields; in particular, experiments were designed to determine whether seed treatments were less effective at low seeding rates. Four experiments were conducted over three years by varying seeding rates of rice treated at constant per seed rates of insecticide. Larval suppression by chlorantraniliprole was superior to thiamethoxam or clothianidin, and infestations at low seeding rates were up to 47% higher than at high seeding rates. Little evidence was found for the hypothesis that seed treatments are less effective at low seeding rates; in only one of four experiments was the reduction in weevil densities by thiamethoxam greater at high than at low seeding rates. However, suppression of larvae by neonicotinoid seed treatments in plots seeded at low rates was generally poor, and caution must be exercised when using the neonicotioids at low seeding rates.

  6. Physicochemical Characteristics of the Seed and Seed Oil of the Potentially Medicinal Plant Ziziphus oenoplia.

    Science.gov (United States)

    Murthy, Hosakatte Niranjana; Joseph, Kadanthottu Sebastian; Madiwal, Abhishek; Gerald, Dinesh Rajen; Badiger, Mahananda; Kolkar, Lakshmi; Hiremath, Reshma

    2017-11-02

    The proximate composition of seeds, physicochemical characteristics, and fatty acid profiles of Ziziphus oenoplia seed oil were determined in this study. The seeds possessed low moisture (4.54%) and high carbohydrate (42.96%) and protein content (40%), making the seed oil suitable for storage and consumption. The saponification value (197.80) of the seed oil makes it a promising source for the soap and shampoo industry. The iodine and saponification values are comparable to those of major edible seed oils such as groundnut and soybean. The high amount of monounsaturated fatty acids (53.41%), especially oleic acid (53.38%), present in the oil makes it a better source for a low-fat diet and may reduce the risk of various heart-related diseases.

  7. Assessment of Seed Germination and Dormancy of Thirty Seeds Lots of

    Directory of Open Access Journals (Sweden)

    H.R Ehyaee

    2012-06-01

    Full Text Available Most seeds of medicinal plants due to ecological adaptation to environmental conditions have several types of dormancy. Hence, it's necessary to recognize ecological factors that affect dormancy and provide optimum conditions for germination in medicinal plant species. Thirty seed lots were used to estimate germination and dormancy of medicinal plants. Treatments were KNO3, (2% and scarification of seeds by sand paper, hypochlorite sodium and removing the seed coat with four replicates of 25 seeds. Maximum and minimum germination observed in H2O for Digitalis purpure 100% and Saponaria officinalis 0%. In KNO3 treatment, Portulaca oleracea had the highest germination of 91% and Hyocyamus niger had no any germinated seeds. In sand paper treatment, the Saponaria officinalis and Datura stramonium had maximum, 33% and minimum 0% germination respectively.

  8. Use of the software Seed Vigor Imaging System (SVIS® for assessing vigor of carrot seeds

    Directory of Open Access Journals (Sweden)

    José Luís de Marchi

    Full Text Available ABSTRACT Seed vigor has traditionally been evaluated by physiological, biochemical and stress tolerance tests. More recently, with the use of computerized image analysis, objective information has become accessible in a relatively short period of time, with less human interference. The aim of this study was to verify the efficiency of computerized seedling image analysis by Seed Vigor Imaging System (SVIS® to detect differences in vigor between carrot (Daucus carota L. seed lots as compared to those provided by traditional vigor tests. Seeds from seven lots from the Brasilia cultivar were subjected to a germination test, first count of germination, speed of germination, accelerated aging with saline solution and seedling emergence; furthermore, a vigor index, growth index and uniformity index were determined by the Seed Vigor Imaging System (SVIS® during four evaluation periods. The results obtained by the computerized seedling analysis (vigor index and growth index show that SVIS® is efficient in assessing carrot seed vigor.

  9. Seed dimorphism, nutrients and salinity differentially affect seed traits of the desert halophyte Suaeda aralocaspica via multiple maternal effects.

    Science.gov (United States)

    Wang, Lei; Baskin, Jerry M; Baskin, Carol C; Cornelissen, J Hans C; Dong, Ming; Huang, Zhenying

    2012-09-25

    Maternal effects may influence a range of seed traits simultaneously and are likely to be context-dependent. Disentangling the interactions of plant phenotype and growth environment on various seed traits is important for understanding regeneration and establishment of species in natural environments. Here, we used the seed-dimorphic plant Suaeda aralocaspica to test the hypothesis that seed traits are regulated by multiple maternal effects. Plants grown from brown seeds had a higher brown:black seed ratio than plants from black seeds, and germination percentage of brown seeds was higher than that of black seeds under all conditions tested. However, the coefficient of variation (CV) for size of black seeds was higher than that of brown seeds. Seeds had the smallest CV at low nutrient and high salinity for plants from brown seeds and at low nutrient and low salinity for plants from black seeds. Low levels of nutrients increased size and germinability of black seeds but did not change the seed morph ratio or size and germinability of brown seeds. High levels of salinity decreased seed size but did not change the seed morph ratio. Seeds from high-salinity maternal plants had a higher germination percentage regardless of level of germination salinity. Our study supports the multiple maternal effects hypothesis. Seed dimorphism, nutrient and salinity interacted in determining a range of seed traits of S. aralocaspica via bet-hedging and anticipatory maternal effects. This study highlights the importance of examining different maternal factors and various offspring traits in studies that estimate maternal effects on regeneration.

  10. Regenerative role of seed banks following an intense soil disturbance

    Science.gov (United States)

    Luzuriaga, Arantzazu L.; Escudero, Adrián; Olano, José Miguel; Loidi, Javier

    2005-02-01

    Our main aim was to determine the contribution of the seed bank to vegetation regeneration following a disturbance consisting in a deep ploughing and a thorough homogenisation of a perennial grassland. In the seed bank prior to disturbance, seed distribution through the vertical soil profile was evaluated to determine the initial seed species structure. Then, several characteristics of the shallow seed bank and the extant vegetation were evaluated prior and following field disturbance: seed species composition and abundance, and species composition of the aboveground vegetation. The contribution of seed rain versus seed bank was evaluated by means of the comparison of the vegetation developed in plots filled with sterilised soil (seed bank removal) and the vegetation developed in non-sterilised plots in the field. The distribution of seeds through the profile indicated a sharp decline in abundance with depth, and it was probably linked to propagule morphology, with small and rounded seeds proner to being buried deeper than larger seeds. In the grassland prior to disturbance, the aboveground vegetation and seed bank species composition showed very low similarity index, most likely because during the 5 years following field abandonment, sheep pressure had caused a faster change in aboveground vegetation species composition than in seed bank species composition. Ploughing and homogenisation of the grassland led to low seed abundance in the shallow soil layer caused by dilution of the seed bank. Regardless of impoverishment in seed abundance and species richness, comparison between sterilised and non-sterilised plots showed that the seed bank acted as an effective source of colonising species and determined the aboveground species composition. To summarise, this study outlines the importance of considering several characteristics of the seed bank, such as species composition and seed abundance, in the understanding of the function of seed bank and dynamics of the

  11. IMPORTANCE OF STORAGE CONDITIONS AND SEED TREATMENT FOR SUNFLOWER HYBRIDS SEEDS GERMINATION

    Directory of Open Access Journals (Sweden)

    Goran Krizmanić

    2014-12-01

    Full Text Available In this research we have determined germination energy and germination of seeds of sunflower hybrids ‘Luka’ and ‘Apolon’, at the beginning of storage and 6, 12 and 18 months after of storage period (2011-2012 in the floor concrete storage at two different air temperatures and humidity (S-1: air temperature 15-18°C and relative air humidity 65-70% as well as in climate chamber (S-2: air temperature 10-12°C and relative air humidity 60-65%, stored in four treatments (Control: processed-untreated seed; T-1: treated with A.I. metalaxyl-M; T-2: treated with A.I. metalaxyl-M + A.I. imidacloprid and T-3: treated with A.I. metalaxyl-M + A.I. clothianidin. Based on the obtained results we have determined that sunflower hybrid ‘Luka’, compared to hybrid ‘Apolon’, in the given storage conditions and with the same seed treatment has 5-8% higher germination energy and seed germination and that in climate chamber both hybrids have 5-7% higher germination energy. Seed treatment of both sunflower hybrids with A.I. imidacloprid maximally reduced initial germination energy and seed germination in all tested periods and conditions of storage. On the average, natural seed, after 18 months of storage did not have better seed quality compared to seed treated with A.I. metalaxyl-M while other treatments had more significant influence on reduction of germination energy and seed germination, 6-15%. On the average, compared to other variants, seeds treated with A.I. metalaxyl-M after 18 months of storage in both storage conditions had higher germination energy by 4-15%, and seed germination by 2-12%.

  12. Effect of GA3 treatment on seed development and seed-related gene expression in grape.

    Directory of Open Access Journals (Sweden)

    Chenxia Cheng

    Full Text Available The phytohormone gibberellic acid (GA3 is widely used in the table grape industry to induce seedlessness in seeded varieties. However, there is a paucity of information concerning the mechanisms by which GAs induce seedlessness in grapes.In an effort to systematically analyze the cause of this GA3-induced seed abortion, we conducted an in depth characterization of two seeded grape cultivars ('Kyoho' and 'Red Globe', along with a seedless cultivar ('Thompson Seedless', following treatment with GA3. In a similar fashion to the seedless control, which exhibited GA3-induced abortion of the seeds 9 days after full bloom (DAF, both 'Kyoho' and 'Red Globe' seeded varieties exhibited complete abortion of the seeds 15 DAF when treated with GA3. Morphological analyses indicated that while fertilization appeared to occur normally following GA3 treatment, as well as in the untreated seedless control cultivar, seed growth eventually ceased. In addition, we found that GA3 application had an effect on redox homeostasis, which could potentially cause cell damage and subsequent seed abortion. Furthermore, we carried out an analysis of antioxidant enzyme activities, as well as transcript levels from various genes believed to be involved in seed development, and found several differences between GA3-treated and untreated controls.Therefore, it seems that the mechanisms driving GA3-induced seedlessness are similar in both seeded and seedless cultivars, and that the observed abortion of seeds may result at least in part from a GA3-induced increase in cell damage caused by reactive oxygen species, a decrease in antioxidant enzymatic activities, and an alteration of the expression of genes related to seed development.

  13. Dependence of rate of germination of teak ( Tectona grandis ) seeds ...

    African Journals Online (AJOL)

    A study was conducted to determine suitable sources of teak (Tectona grandis) seeds and methods of treating the seeds to promote higher rate of germination, with the objective to supply large quantities of seedlings for developing commercial teak plantations in Ghana. The field work involved seed collection, seed pericarp ...

  14. DETERMINATION OF INHIBITORS, DORMANCY BREAKING AND SEED HEALTH IN CUTIEIRA

    Directory of Open Access Journals (Sweden)

    José Maria Gomes Neves2

    2013-12-01

    was completely randomized, factorial 2 x 5, with two types of seed from freshly harvested fruits and remnants and five pre-germination treatments. The seed coat removal promotes increased germination and seed vigor derived from harvested fruits. Fungi identified in the cutieira seeds were Aspergillus niger, Aspergillus flavus, Penicillium sp. and Rhizopus sp.

  15. Identification of seed-related QTL in Brassica rapa

    Directory of Open Access Journals (Sweden)

    H. Bagheri

    2013-10-01

    Full Text Available To reveal the genetic variation, and loci involved, for a range of seed-related traits, a new F2 mapping population was developed by crossing Brassica rapa ssp. parachinensis L58 (CaiXin with B. rapa ssp. trilocularis R-o-18 (spring oil seed, both rapid flowering and self-compatible. A linkage map was constructed using 97 AFLPs and 21 SSRs, covering a map distance of 757 cM with an average resolution of 6.4 cM, and 13 quantitative trait loci (QTL were detected for nine traits. A strong seed colour QTL (LOD 26 co-localized with QTL for seed size (LOD 7, seed weight (LOD 4.6, seed oil content (LOD 6.6, number of siliques (LOD 3 and number of seeds per silique (LOD 3. There was only a significant positive correlation between seed colour and seed oil content in the yellow coloured classes. Seed coat colour and seed size were controlled by the maternal plant genotype. Plants with more siliques tended to have more, but smaller, seeds and higher seed oil content. Seed colour and seed oil content appeared to be controlled by two closely linked loci in repulsion phase. Thus, it may not always be advantageous to select for yellow-seededness when breeding for high seed oil content in Brassicas.

  16. The impact of groundwater level on soil seed bank survival

    NARCIS (Netherlands)

    Bekker, RM; Oomes, MJM; Bakker, JP

    Seed longevity of plant species is an important topic in restoration management, and little is known about the effects of environmental conditions on seed survival and longevity under natural conditions. Therefore, the effect of groundwater level on the survival of seeds in the soil seed bank of a

  17. Thieving rodents as substitute dispersers of megafaunal seeds

    Science.gov (United States)

    Jansen, Patrick A.; Hirsch, Ben T.; Emsens, Willem-Jan; Zamora-Gutierrez, Veronica; Wikelski, Martin; Kays, Roland

    2012-01-01

    The Neotropics have many plant species that seem to be adapted for seed dispersal by megafauna that went extinct in the late Pleistocene. Given the crucial importance of seed dispersal for plant persistence, it remains a mystery how these plants have survived more than 10,000 y without their mutualist dispersers. Here we present support for the hypothesis that secondary seed dispersal by scatter-hoarding rodents has facilitated the persistence of these large-seeded species. We used miniature radio transmitters to track the dispersal of reputedly megafaunal seeds by Central American agoutis, which scatter-hoard seeds in shallow caches in the soil throughout the forest. We found that seeds were initially cached at mostly short distances and then quickly dug up again. However, rather than eating the recovered seeds, agoutis continued to move and recache the seeds, up to 36 times. Agoutis dispersed an estimated 35% of seeds for >100 m. An estimated 14% of the cached seeds survived to the next year, when a new fruit crop became available to the rodents. Serial video-monitoring of cached seeds revealed that the stepwise dispersal was caused by agoutis repeatedly stealing and recaching each other’s buried seeds. Although previous studies suggest that rodents are poor dispersers, we demonstrate that communities of rodents can in fact provide highly effective long-distance seed dispersal. Our findings suggest that thieving scatter-hoarding rodents could substitute for extinct megafaunal seed dispersers of tropical large-seeded trees. PMID:22802644

  18. Gene regulation in seeds : insights into translational dynamics

    NARCIS (Netherlands)

    Bai, B.

    2016-01-01

    Seeds are unique structures in the plant life cycle. The variation in the timing of seed maturation, dispersion, and the establishment of seed dormancy and longevity, increases the chances of plant survival and enlarge the distance that plants could disperse in the natural habitat. Seeds contain

  19. Soybean seed viability and changes of fatty acids content as ...

    African Journals Online (AJOL)

    The characteristics of soybean seed chemical composition are related to specific processes occurring in seed during storage. These changes lead to seed aging during storage and affect seed vigour and content of fatty acids. In order to reveal severity of their influence, the following vigour tests were applied: Standard ...

  20. 7 CFR 201.54 - Number of seeds for germination.

    Science.gov (United States)

    2010-01-01

    ... REGULATIONS Germination Tests in the Administration of the Act § 201.54 Number of seeds for germination. At least 400 seeds shall be tested for germination; except that in mixtures, 200 seeds of each of those... 7 Agriculture 3 2010-01-01 2010-01-01 false Number of seeds for germination. 201.54 Section 201.54...

  1. Burial increases seed longevity of two Artemisia tridentata (Asteraceae) subspecies

    Science.gov (United States)

    Wijayratne, Upekala C.; Pyke, David A.

    2012-01-01

    Premise of the study: Seed longevity and persistence in soil seed banks may be especially important for population persistence in ecosystems where opportunities for seedling establishment and disturbance are unpredictable. The fire regime, an important driver of population dynamics in sagebrush steppe ecosystems, has been altered by exotic annual grass invasion. Soil seed banks may play an active role in postfire recovery of the foundation shrub Artemisia tridentata, yet conditions under which seeds persist are largely unknown. Methods: We investigated seed longevity of two Artemisia tridentata subspecies in situ by retrieving seed bags that were placed at varying depths over a 2 yr period. We also sampled naturally dispersed seeds in litter and soil immediately after seed dispersal and before flowering in subsequent seasons to estimate seed persistence. Key results: After 24 mo, seeds buried at least 3 cm below the soil surface retained 30–40% viability whereas viability of seeds on the surface and under litter declined to 0 and Artemisia tridentata has the potential to form a short-term soil seed bank that persists longer than has been commonly assumed, and that burial is necessary for seed longevity. Use of seeding techniques that promote burial of some seeds to aid in formation of a soil seed bank may increase restoration potential.

  2. 7 CFR 201.47a - Seed unit.

    Science.gov (United States)

    2010-01-01

    ... Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT... structure usually regarded as a seed in planting practices and in commercial channels. The seed unit may...

  3. Effects of hydropriming on seed germination and seedling growth in ...

    African Journals Online (AJOL)

    The germination of Salvia officinalis L. (sage) seeds is a problem of great concern that may be overcome by employing seed priming techniques. Seed priming is an efficient technique for improvement of seed vigor, increasing germination and seedling growth. Little information has been reported on seedling development ...

  4. Abscisic acid and assimilate partitioning during seed development

    NARCIS (Netherlands)

    Bruijn, de S.M.

    1993-01-01

    This thesis describes the influence of abscisic acid (ABA) on the transport of assimilates to seeds and the deposition of reserves in seeds. It is well-known from literature that ABA accumulates in seeds during development, and that ABA concentrations in seeds correlate rather well with

  5. Viability Prediction of Ricinus cummunis L. Seeds Using Multispectral Imaging

    DEFF Research Database (Denmark)

    Olesen, Merete Halkjær; Nikneshan, Pejman; Shrestha, Santosh

    2015-01-01

    The purpose of this study was to highlight the use of multispectral imaging in seed quality testing of castor seeds. Visually, 120 seeds were divided into three classes: yellow, grey and black seeds. Thereafter, images at 19 different wavelengths ranging from 375–970 nm were captured of all the s...

  6. Comparative studies of drying methods on the seed quality of ...

    African Journals Online (AJOL)

    Seed moisture content (MC) is a key component that determines storability of germplasm conserved in seed genebanks. The purpose of this research was to compare the efficiency of sun-, shade-, silica geland conventional room drying in terms of rice seed MC and viability using seeds of two interspecific progenies ...

  7. Ethanol exposure can inhibit red spruce ( Picea rubens ) seed germination

    Science.gov (United States)

    John R. Butnor; Brittany M. Verrico; Victor Vankus; Stephen R. Keller

    2018-01-01

    Flotation of seeds in solvents is a common means of separating unfilled and filled seeds. While a few protocols for processing red spruce (Picea rubens) seeds recommend ethanol flotation, delayed and reduced germination have been reported. We conducted an ethanol bioassay on seeds previously stored at -20°C to quantify the concentration required to separate red spruce...

  8. Factors Affecting Tocopherol Concentrations in Soybean Seeds.

    Science.gov (United States)

    Carrera, Constanza S; Seguin, Philippe

    2016-12-21

    Soybean seeds contain several health-beneficial compounds, including tocopherols, which are used by the nutraceutical and functional food industries. Soybean tocopherol concentrations are, however, highly variable. Large differences observed in tocopherol concentrations among soybean genotypes together with the relatively simple biosynthetic pathway involving few genes support the feasibility of selecting for high-tocopherol soybean. Tocopherol concentrations are also highly influenced by environmental factors and field management. Temperature during seed filling and soil moisture appear to be the main factors affecting tocopherol concentrations; other factors such as soil fertility and solar radiation also affect concentrations and composition. Field management decisions including seeding date, row spacing, irrigation, and fertilization also affect tocopherols. Knowledge of factors affecting soybean tocopherols is essential to develop management strategies that will lead to the production of seeds with consistent target concentrations that will meet the needs of the nutraceutical and functional food industries.

  9. Chickpea seeds germination rational parameters optimization

    Science.gov (United States)

    Safonova, Yu A.; Ivliev, M. N.; Lemeshkin, A. V.

    2018-05-01

    The paper presents the influence of chickpea seeds bioactivation parameters on their enzymatic activity experimental results. Optimal bioactivation process modes were obtained by regression-factor analysis: process temperature - 13.6 °C, process duration - 71.5 h. It was found that in the germination process, the proteolytic, amylolytic and lipolytic enzymes activity increased, and the urease enzyme activity is reduced. The dependences of enzyme activity on chickpea seeds germination conditions were obtained by mathematical processing of experimental data. The calculated data are in good agreement with the experimental ones. This confirms the optimization efficiency based on experiments mathematical planning in order to determine the enzymatic activity of chickpea seeds germination optimal parameters of bioactivated seeds.

  10. Effects of gamma radiation in tomato seeds

    Energy Technology Data Exchange (ETDEWEB)

    Wiendl, Toni A.; Wiendl, Fritz W.; Franco, Suely S.H.; Franco, Jose G.; Althur, Valter, E-mail: tawiendl@hotmail.com, E-mail: gilmita@uol.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Arthur, Paula B., E-mail: arthur@cena.usp.br [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)

    2013-07-01

    Tomato dry seeds of the hybrid 'Gladiador' F1 were exposed to low doses of gamma radiation from Co-60 source at 0,509 kGy tax rate in order to study stimulation effects of radiation on germination and plant growth. Eight treatments radiation doses were applied as follows: 0 (control); 2,5; 5,0; 7,5; 10,0; 12,5; 15,0; 20,0 Gy. Seed germination as well as green fruits number, harvested fruit number, fruit weight and total production were assessed to identify occurrence of stimulation. Tomato seeds and plants were handled as for usual tomato production in Brazil. Low doses of gamma radiation treatment in the seeds stimulate germination and substantially increase fruit number and total production up to 86% at 10 Gy dose. There are evidences that the use of low doses of gamma radiation can stimulate germination and plant production thus, showing hormetic effects. (author)

  11. Shaping metal nanocrystals through epitaxial seeded growth

    Energy Technology Data Exchange (ETDEWEB)

    Habas, Susan E.; Lee, Hyunjoo; Radmilovic, Velimir; Somorjai,Gabor A.; Yang, Peidong

    2008-02-17

    Morphological control of nanocrystals has becomeincreasingly important, as many of their physical and chemical propertiesare highly shape-dependent. Nanocrystal shape control for both single andmultiple material systems, however, remains fairly empirical andchallenging. New methods need to be explored for the rational syntheticdesign of heterostructures with controlled morphology. Overgrowth of adifferent material on well-faceted seeds, for example, allows for the useof the defined seed morphology to control nucleation and growth of thesecondary structure. Here, we have used highly faceted cubic Pt seeds todirect the epitaxial overgrowth of a secondary metal. We demonstrate thisconcept with lattice matched Pd to produce conformal shape-controlledcore-shell particles, and then extend it to lattice mismatched Au to giveanisotropic growth. Seeding with faceted nanocrystals may havesignificant potential towards the development of shape-controlledheterostructures with defined interfaces.

  12. Portulaca oleracea Linn seed extract ameliorates hydrogen ...

    African Journals Online (AJOL)

    Portulaca oleracea Linn seed extract ameliorates hydrogen ... induced cell death by inhibiting oxidative stress and ROS generation. Keywords: ... culture medium; therefore the stock solutions of ... acetic acid (1 %) and ethanol (50 %) to extract.

  13. Effects of gamma radiation in tomato seeds

    International Nuclear Information System (INIS)

    Wiendl, Toni A.; Wiendl, Fritz W.; Franco, Suely S.H.; Franco, Jose G.; Althur, Valter; Arthur, Paula B.

    2013-01-01

    Tomato dry seeds of the hybrid 'Gladiador' F1 were exposed to low doses of gamma radiation from Co-60 source at 0,509 kGy tax rate in order to study stimulation effects of radiation on germination and plant growth. Eight treatments radiation doses were applied as follows: 0 (control); 2,5; 5,0; 7,5; 10,0; 12,5; 15,0; 20,0 Gy. Seed germination as well as green fruits number, harvested fruit number, fruit weight and total production were assessed to identify occurrence of stimulation. Tomato seeds and plants were handled as for usual tomato production in Brazil. Low doses of gamma radiation treatment in the seeds stimulate germination and substantially increase fruit number and total production up to 86% at 10 Gy dose. There are evidences that the use of low doses of gamma radiation can stimulate germination and plant production thus, showing hormetic effects. (author)

  14. NUTRIENT COMPOSITION OF NIGER SEED (GUIZOTIA ...

    African Journals Online (AJOL)

    B. S. Chandravanshi

    fiber. Niger oil has a fatty acid composition typical for seed oils of the .... system using external calibration curve after optimizing the parameters in to .... physical and chemical properties of the soil, application of natural (manure) and artificial.

  15. Compact 2 Micron Seed Laser, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — This proposal is for the development of new compact, high power and extremely reliable 2 micron seed laser using newly developed Tm3+ doped germanate glass fibers,...

  16. Compact 2 Micron Seed Laser, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — This proposal is for the development of innovative compact, high power and extremely reliable 2 micron seed laser using newly developed Tm3+ doped germanate glass...

  17. Seed germination of Hippophae salicifolia D

    African Journals Online (AJOL)

    Deepak Dhyani

    2012-11-08

    Nov 8, 2012 ... lead towards the development of a simple cost-effective propagation technique for large scale cultivation and ... Propagation of the species by seed is time consuming technique ..... In: Singh, V. (Eds.), Botany,. Harvesting and ...

  18. Seeds that give: Participatory plant breeding

    International Development Research Centre (IDRC) Digital Library (Canada)

    IDRC

    Faced with a unique economic crisis, Cuba's national agriculture system was near collapse ... tion, and distribution of improved maize and bean seeds. A secondary but ... ers rate 50 percent of the newly introduced varieties to be superior to ...

  19. Seed reserve utilization and hydrolytic enzyme activities in germinating seeds of sweet corn

    International Nuclear Information System (INIS)

    Cheng, X.; Xiong, F.; Wang, C.; He, S.; Zhou, Y.

    2018-01-01

    In this study, two sh2 sweet corn cultivars (i.e., the initial seed dry weight for FT018 and TB010 was 0.16+-0.02 g/grain and 0.09+-0.01 g/grain, respectively) were used to determine the physiological characteristics of seed reserve utilization in germination. The data implied that the weight of mobilized seed reserve (WMSR) and seed reserve utilization efficiency (SRUE) increased with seed germination. FT018 exhibited higher SRUE than TB010 due to its sufficient energy production for growth. Sugar (sucrose and fructose) contents were at different levels in the germinating seed of sh2 sweet corn. The protein content and number of protein species were highest in the early stage of germination. Enzyme activity in the germinating seed indicated that enzymes for starch and sugar hydrolysis were important and that enzyme activities significantly differed at each germination stage and between the cultivars under dark conditions. Succinate dehydrogenase, sucrose synthase, and glucose-6-phosphate dehydrogenase accumulated in the late germination stage. Thus, appropriate efforts should be focused on improving the seed reserve utilization in sweet corn by identifying the physiological mechanism of germinating seed. (author)

  20. Induction of 9-cis-epoxycarotenoid dioxygenase in Arabidopsis thaliana seeds enhances seed dormancy.

    Science.gov (United States)

    Martínez-Andújar, Cristina; Ordiz, M Isabel; Huang, Zhonglian; Nonogaki, Mariko; Beachy, Roger N; Nonogaki, Hiroyuki

    2011-10-11

    Full understanding of mechanisms that control seed dormancy and germination remains elusive. Whereas it has been proposed that translational control plays a predominant role in germination, other studies suggest the importance of specific gene expression patterns in imbibed seeds. Transgenic plants were developed to permit conditional expression of a gene encoding 9-cis-epoxycarotenoid dioxygenase 6 (NCED6), a rate-limiting enzyme in abscisic acid (ABA) biosynthesis, using the ecdysone receptor-based plant gene switch system and the ligand methoxyfenozide. Induction of NCED6 during imbibition increased ABA levels more than 20-fold and was sufficient to prevent seed germination. Germination suppression was prevented by fluridone, an inhibitor of ABA biosynthesis. In another study, induction of the NCED6 gene in transgenic seeds of nondormant mutants tt3 and tt4 reestablished seed dormancy. Furthermore, inducing expression of NCED6 during seed development suppressed vivipary, precocious germination of developing seeds. These results indicate that expression of a hormone metabolism gene in seeds can be a sole determinant of dormancy. This study opens the possibility of developing a robust technology to suppress or promote seed germination through engineering pathways of hormone metabolism.

  1. Water Productivity of Irrigated Rice under Transplanting, Wet Seeding and Dry Seeding Methods of Cultivation

    Directory of Open Access Journals (Sweden)

    Murali, NS.

    1997-01-01

    Full Text Available Water productivity (WP of irrigated lowland rice was determined during the 1994 dry (January to May and wet (August to December seasons on a heavy clay acid sulphate soil. Treatments consisted of three cultivation methods : transplanted rice, pregerminated seeds broadcasted on puddled soil (wet seeding and dry seeds broadcasted on unpuddled soil (dry seeding. In wet and dry seeded plots, continuous standing water condition was initiated 17 days after sowing. Total water requirement for rice production was highest in transplanted plots (755 mm in wet season and 1154 mm in dry season and was lowest in dry seeded plots (505 mm in wet season and 1040 mm in dry season. Dry seeding required no water for land preparation but transplanting and wet seeding methods required 18 - 20 % of total water requirement in dry season and 27 - 29 % in wet season. Total percolation was maximum (99 mm in wet season and 215 mm in dry season in dry seeding method and was minimum (62 mm in wet season and 94 mm in dry season in transplanting method. In dry and wet seeding methods, daily percolation gradually decreased with the age of the crop. Total seepage loss did not show any significant difference between the cultivation methods in the two seasons. Grain yield was not affected by the three cultivation methods in both seasons. Water productivity (the ratio between grain yield and total amount of water used in production was 3.5 - 4.1 kg ha-1 mm-1, 3.8 - 4.4 kg ha-1 mm-1 and 4.1 - 5.5 kg ha-1 mm-1 in transplanted, wet seeded and dry seeded rice, respectively. Labour requirement for land preparation and sowing was maximum in transplanted (219 - 226 man-hours ha-1 followed by wet (104 -112 man-hours ha-1 and dry seeded (94 - 99 man-hours ha-1 methods. However, in wet season extra labour (77 man-hours ha-1 was required for weeding after crop establishment in dry and wet seeding methods. Crop maturity was 20 days earlier in wet and dry seeding methods compared to

  2. Development of nutritious snacks by incorporation of amaranth seeds, watermelon seeds and their flour

    OpenAIRE

    Paul Virginia; Ruchi .; Paul Ajit

    2014-01-01

    The present study was carried out with the objectives to find out the sensory acceptability, the nutrient content and cost of prepared products. The products prepared were “Biscuits”, “Mathri” and “Laddoo” by incorporation of amaranth seeds, watermelon seeds and their flour in different proportions (10:10, 20:10, and 30:10) served as treatments T1, T2 and T3 respectively T0, without incorporation of amaranth seeds, watermelon seeds and their flour served as control. The products were organole...

  3. Seed dispersers, seed predators, and browsers act synergistically as biotic filters in a mosaic landscape.

    Directory of Open Access Journals (Sweden)

    Regino Zamora

    Full Text Available In this study, we analize the functional influence of animals on the plants they interact with in a mediterranean mountain. We hypothesise that seed dispersers, seed predators, and browsers can act as biotic filters for plant communities. We analyse the combined effects of mutualistic (seed dispersal and antagonistic (seed predation, herbivory animal interactions in a mosaic landscape of Mediterranean mountains, basing our results on observational and experimental field. Most of the dispersed seeds came from tree species, whereas the population of saplings was composed predominantly of zoochorous shrub species. Seed predators preferentially consumed seeds from tree species, whereas seeds from the dominant fleshy-fruited shrubs had a higher probability of escaping these predators. The same pattern was repeated among the different landscape units by browsers, since they browsed selectively and far more intensely on tree-species saplings than on the surrounding shrubs. In synthesis, our work identifies the major biotic processes that appear to be favoring a community dominated by shrubs versus trees because seed dispersers, predators, and herbivores together favored shrub dispersal and establishment versus trees.

  4. Phylogenetic diversification of Early Cretaceous seed plants: The compound seed cone of Doylea tetrahedrasperma.

    Science.gov (United States)

    Rothwell, Gar W; Stockey, Ruth A

    2016-05-01

    Discovery of cupulate ovules of Doylea tetrahedrasperma within a compact, compound seed cone highlights the rich diversity of fructification morphologies, pollination biologies, postpollination enclosure of seeds, and systematic diversity of Early Cretaceous gymnosperms. Specimens were studied using the cellulose acetate peel technique, three-dimensional reconstructions (in AVIZO), and morphological phylogenetic analyses (in TNT). Doylea tetrahedrasperma has bract/fertile short shoot complexes helically arranged within a compact, compound seed cone. Complexes diverge from the axis as a single unit and separate distally into a free bract tip and two sporophylls. Each sporophyll bears a single, abaxial seed, recurved toward the cone axis, that is enveloped after pollinaton by sporophyll tissue, forming a closed cupule. Ovules are pollinated by bisaccate grains captured by micropylar pollination horns. The unique combination of characters shown by D. tetrahedrasperma includes the presence of cupulate seeds borne in conifer-like compound seed cones, an ovuliferous scale analogue structurally equivalent to the ovulate stalk of Ginkgo biloba, gymnospermous pollination, and nearly complete enclosure of mature seeds. These features characterize the Doyleales ord. nov., clearly distinguish it from the seed fern order Corystospermales, and allow for recognition of another recently described Early Cretaceous seed plant as a second species in genus Doylea. A morphological phylogenetic analysis highlights systematic relationships of the Doyleales ord. nov. and emphasizes the explosive phylogenetic diversification of gymnosperms that was underway at the time when flowering plants may have originated and/or first began to radiate. © 2016 Botanical Society of America.

  5. Device for isolation of seed crystals during processing of solution

    Science.gov (United States)

    Montgomery, K.E.; Zaitseva, N.P.; Deyoreo, J.J.; Vital, R.L.

    1999-05-18

    A device is described for isolation of seed crystals during processing of solutions. The device enables a seed crystal to be introduced into the solution without exposing the solution to contaminants or to sources of drying and cooling. The device constitutes a seed protector which allows the seed to be present in the growth solution during filtration and overheating operations while at the same time preventing the seed from being dissolved by the under saturated solution. When the solution processing has been completed and the solution cooled to near the saturation point, the seed protector is opened, exposing the seed to the solution and allowing growth to begin. 3 figs.

  6. Seed germination and sowing options [Chapter 8

    Science.gov (United States)

    Tara Luna; Kim Wilkinson; R. Kasten Dumroese

    2009-01-01

    Seeds of many native species are challenging to germinate. One important thing a grower can do is learn as much as possible about the life history, ecology, and habitat of the species they wish to grow.What processes do seeds of this species go through in nature? Any observations will be valuable when trying to germinate and grow species that have little or no...

  7. Distribution of droplet sizes for seed solution

    International Nuclear Information System (INIS)

    Marwah, R.K.; Dixit, N.S.; Venkataramani, N.; Rohatgi, V.K.

    In open cycle MHD power generation, power is generated by passing seeded hot combustion products of a fossil fuel through a magnetic field. Seeding is done with a salt which is readily ionizable, preferably in the form of an aqueous solution, such as potassium carbonate, potassium sulphate, etc. Methods of atomization and the theoretical drop size calculations are presented. Basic parameters necessary for droplet size determination and their measurement are also described. (K.B.)

  8. Mathematical model of seed germination process

    International Nuclear Information System (INIS)

    Gładyszewska, B.; Koper, R.; Kornarzyński, K.

    1999-01-01

    An analytical model of seed germination process was described. The model based on proposed working hypothesis leads - by analogy - to a law corresponding with Verhulst-Pearl's law, known from the theory of population kinetics. The model was applied to describe the germination kinetics of tomato seeds, Promyk field cultivar, biostimulated by laser treatment. Close agreement of experimental and model data was obtained [pl

  9. Fluff-thieving birds sabotage seed dispersal

    OpenAIRE

    Rohwer, Vanya G.; Pauw, Anton; Martin, Paul R.

    2017-01-01

    Characterizing many species interactions as mutualisms can be misleading because some members of the interaction derive greater fitness benefits at the expense of other members. We provide detailed natural history data on a suspected bird?plant mutualism in South Africa where many species of birds use fluffy Eriocephalus seed material to construct their nests, potentially dispersing seeds for the plant. We focus on a common bird, Prinia maculosa, which invests heavily in gathering Eriocephalu...

  10. Quantized Matrix Algebras and Quantum Seeds

    DEFF Research Database (Denmark)

    Jakobsen, Hans Plesner; Pagani, Chiara

    2015-01-01

    We determine explicitly quantum seeds for classes of quantized matrix algebras. Furthermore, we obtain results on centres and block diagonal forms of these algebras. In the case where is an arbitrary root of unity, this further determines the degrees.......We determine explicitly quantum seeds for classes of quantized matrix algebras. Furthermore, we obtain results on centres and block diagonal forms of these algebras. In the case where is an arbitrary root of unity, this further determines the degrees....

  11. Lettuce seeds production in hydroponic system

    OpenAIRE

    Menezes, Nilson Lemos de; Santos, Osmar Souza dos; Schmidt, Denise

    2001-01-01

    Sementes de alface das cultivares Deyse e Regina foram produzidas em estufa, no sistema hidropônico, com elevados rendimentos por planta e boa qualidade de sementes, quando comparados a resultados de sistema convencional, de canteiros em campo. Esses dados sugerem estudos de produção de sementes de alface em cultivo protegido.Lettuce seeds of Deyse and Regina cultivars were produced in greenhouse, in hydroponic system, with excelents rendiments per plant and seed quality, when compared to con...

  12. Seed Potato Production and Its Importance

    OpenAIRE

    Erdoğan ÖZTÜRK; Taşkın POLAT

    2017-01-01

    Our country has different agricultural regions showed different ecological properties in terms of climate and soil characteristics increase the plant variety. Within this variety the potato is one of the most important plants for agriculture and economic of our country, also it is an important food source for human. It is important to use productive and quality seed for healthy agricultural production. With the using of good quality seed, can be obtained about 20% increase in yield. Certified...

  13. [Testing methods for seed quality of Bletilla striata].

    Science.gov (United States)

    Zhang, Zhi-Hui; Liu, Da-Hui; Zhu, Xin-Yan; Ji, Peng-Zhang; Wang, Li; Shi, Ya-Na; Ma, Cong-Ji

    2016-06-01

    In order to provide a basis for establishing seed testing rules and seed quality standard of Bletilla striata, the seed quality of B.striata from different producing area was measured referring to the Rules for Agricultural Seed Testing(GB/T 3543-1995).The results showed that the seeds of B.striata passed through 20-mesh sieve for purity analysis.The weight of seeds was measured by 1000-seed method and the water content was measured at the higher temperature (133±2) ℃ for 3 hours.The seeds were cultured on the wet filter paper at 30 ℃ for 4-20 days in light for germination testing.The method of testing seed viability was that seeds were dipped into 1% TTC solution for 7 hours at temperature of 40 ℃. Copyright© by the Chinese Pharmaceutical Association.

  14. Use of multispectral images and chemometrics in tomato seed studies

    DEFF Research Database (Denmark)

    Shrestha, Santosh; Deleuran, Lise Christina; Gislum, René

    During the production of tomato seeds, green tomatoes are normally discarded before seed extraction irrespective of their maturity stage. Studies indicate that seeds from green tomatoes may reach be able to reach full germination capacity. Thus the potential of multispectral imaging for non......-destructive discrimination of seeds based on their germination capacity was investigated. A total of 840 seeds extracted from green and red tomatoes were divided into two sets; a training set and a test set consisting of 648 and 192 seeds respectively. Each set consisted of 96 seeds from green tomatoes. The multispectral...... images of the seeds were captured and normalized canonical discriminant analysis was used to analyse the images. Germination tests were performed and seeds that subsequently germinated were recorded as viable. The viable seeds were classified with 99% and 98% accuracy for the training and test set...

  15. Testing Optimum Seeding Rates for five Bread Wheat Cultivars

    International Nuclear Information System (INIS)

    Wekesa, S.J.; Kiriswa, F.; Owuoche, J.

    1999-01-01

    A cultivar by seed rate trial was conducted in 1994-1995 crop seasons at Njoro, Kenya. Yield results were found to be significant (P > 0.01) for year, variety, seed rate and year by seed rate interaction. Test weight was highly significant (P -1 were grouped together for significantly higher yields (A) whereas seed rates 85 and 50 kg ha -1 had lower significant yields (B and C respectively). The same grouping was repeated for test weight. There was no significant cultivar by seed rate interaction and no cultivar, specific seed rate. However, since seed rates 245, 205, 165 and 125 kg ha -1 were grouped together, the lowest seed rate, 125 kg ha -1 can be recommended as the optimum seed rate for the above cultivars, as higher seed rates do not give significantly higher yields or higher test weights

  16. Weed seed predation in organic and conventional fields

    DEFF Research Database (Denmark)

    Navntoft, Søren; Wratten, S.D.; Kristensen, Kristian

    2009-01-01

    Enhanced biological control of weed seeds may improve sustainability of agricultural production. Biological control due to seed predation may be higher in organic fields because organic production generally supports more seed predators. To investigate such a difference, weed seed predation...... University and in two of the fields used for estimating seed predation. Recording of predators had therefore limited overlap with seed predation assays but was expected to give important information on key seed predators in the region. The mean seed removal rate was 17% in organic fields compared with 10...... edges. Overall, there was no consistent effect of distance from the field edge. Vegetation had a significant influence on the predation rates, with maximum rates at a medium-dense plant cover. Based on the video images, birds were the most important seed predators. The higher weed seed predation rate...

  17. Effect of Time and Burial Depth on Breaking Seed dormancy and Germination of Weed Seeds

    Directory of Open Access Journals (Sweden)

    marzie mazhari

    2016-02-01

    Full Text Available Introduction: Weeds limit crop growth, development and yield through competing. Seed bank of weeds in field is one of the sources which can affect weed management and their control methods. Environmental conditions during seed maturation and following dispersal interact to influence the germination phenology of many species. Disturbance plays a key role in the maintenance of habitat for many plant species, particularly referrals, for example, fire ephemerals, desert annuals, and arable weeds. Seed germination and emergence depend on endogenous and exogenous factors. Viable seeds are dormant when all environmental conditions are appropriate for germination but seeds fail to germinate. Thus, dormancy plays an important ecological role in preventing seed germination, being a major contributor to seed persistence of some species in soil. Buried seeds of annual weeds are certainly subjected to different soil moisture conditions during their dormancy release season (winter according to the annual rainfall pattern and burial depth. Shallow buried seeds are exposed to soil moisture fluctuations that could affect their dormancy status. Laboratory studies showed that desiccation and subsequent re-hydration of seeds could stimulate germination and modify seed light requirements. Seeds buried in deeper layers of the soil would not be exposed to such fluctuations in soil moisture, but would be exposed to different soil moisture environments depending on weather and soil characteristics. The effects of interactions between temperature, and soil or seed moisture, on seed dormancy changes have been reported for several species. Therefore, the objectives of this study were to determine the effect of time and burial depth treatments on seed germination and seedling emergence of Aegilops cylindrica, Agropyrom repens, Avena fatua, Bromus dantoniae, Cynodon dactylon, Cyprus rotundus, Setaria viridis, Anthriscus sylvestris, Centurea cyanus. Materials and Methods: In

  18. Maximizing the phylogenetic diversity of seed banks.

    Science.gov (United States)

    Griffiths, Kate E; Balding, Sharon T; Dickie, John B; Lewis, Gwilym P; Pearce, Tim R; Grenyer, Richard

    2015-04-01

    Ex situ conservation efforts such as those of zoos, botanical gardens, and seed banks will form a vital complement to in situ conservation actions over the coming decades. It is therefore necessary to pay the same attention to the biological diversity represented in ex situ conservation facilities as is often paid to protected-area networks. Building the phylogenetic diversity of ex situ collections will strengthen our capacity to respond to biodiversity loss. Since 2000, the Millennium Seed Bank Partnership has banked seed from 14% of the world's plant species. We assessed the taxonomic, geographic, and phylogenetic diversity of the Millennium Seed Bank collection of legumes (Leguminosae). We compared the collection with all known legume genera, their known geographic range (at country and regional levels), and a genus-level phylogeny of the legume family constructed for this study. Over half the phylogenetic diversity of legumes at the genus level was represented in the Millennium Seed Bank. However, pragmatic prioritization of species of economic importance and endangerment has led to the banking of a less-than-optimal phylogenetic diversity and prioritization of range-restricted species risks an underdispersed collection. The current state of the phylogenetic diversity of legumes in the Millennium Seed Bank could be substantially improved through the strategic banking of relatively few additional taxa. Our method draws on tools that are widely applied to in situ conservation planning, and it can be used to evaluate and improve the phylogenetic diversity of ex situ collections. © 2014 Society for Conservation Biology.

  19. Fluff-thieving birds sabotage seed dispersal.

    Science.gov (United States)

    Rohwer, Vanya G; Pauw, Anton; Martin, Paul R

    2017-01-01

    Characterizing many species interactions as mutualisms can be misleading because some members of the interaction derive greater fitness benefits at the expense of other members. We provide detailed natural history data on a suspected bird-plant mutualism in South Africa where many species of birds use fluffy Eriocephalus seed material to construct their nests, potentially dispersing seeds for the plant. We focus on a common bird, Prinia maculosa , which invests heavily in gathering Eriocephalus material. Prinias spent 5 of their median 6-day nest construction period adding seed material to their nests and frequently travelled outside their territory boundary to gather Eriocephalus material. Yet, prinias gathered primarily Eriocephalus fluff and actively avoided gathering seeds. The average prinia nest contained only 6.6 seeds, but contained fluff from 579 seeds. These data suggest that prinias provide limited dispersal benefits to Eriocephalus plants. By contrast, the large amounts of Eriocephalus fluff in prinia nests, and the effort that prinias invest in gathering it, suggest that prinias benefit from constructing their nests with Eriocephalus material. We end by outlining hypotheses for possible fitness benefits that Eriocephalus material could provide prinias and other birds.

  20. Precipitation formation from orographic cloud seeding.

    Science.gov (United States)

    French, Jeffrey R; Friedrich, Katja; Tessendorf, Sarah A; Rauber, Robert M; Geerts, Bart; Rasmussen, Roy M; Xue, Lulin; Kunkel, Melvin L; Blestrud, Derek R

    2018-02-06

    Throughout the western United States and other semiarid mountainous regions across the globe, water supplies are fed primarily through the melting of snowpack. Growing populations place higher demands on water, while warmer winters and earlier springs reduce its supply. Water managers are tantalized by the prospect of cloud seeding as a way to increase winter snowfall, thereby shifting the balance between water supply and demand. Little direct scientific evidence exists that confirms even the basic physical hypothesis upon which cloud seeding relies. The intent of glaciogenic seeding of orographic clouds is to introduce aerosol into a cloud to alter the natural development of cloud particles and enhance wintertime precipitation in a targeted region. The hypothesized chain of events begins with the introduction of silver iodide aerosol into cloud regions containing supercooled liquid water, leading to the nucleation of ice crystals, followed by ice particle growth to sizes sufficiently large such that snow falls to the ground. Despite numerous experiments spanning several decades, no direct observations of this process exist. Here, measurements from radars and aircraft-mounted cloud physics probes are presented that together show the initiation, growth, and fallout to the mountain surface of ice crystals resulting from glaciogenic seeding. These data, by themselves, do not address the question of cloud seeding efficacy, but rather form a critical set of observations necessary for such investigations. These observations are unambiguous and provide details of the physical chain of events following the introduction of glaciogenic cloud seeding aerosol into supercooled liquid orographic clouds.

  1. Fluff-thieving birds sabotage seed dispersal

    Science.gov (United States)

    2017-01-01

    Characterizing many species interactions as mutualisms can be misleading because some members of the interaction derive greater fitness benefits at the expense of other members. We provide detailed natural history data on a suspected bird–plant mutualism in South Africa where many species of birds use fluffy Eriocephalus seed material to construct their nests, potentially dispersing seeds for the plant. We focus on a common bird, Prinia maculosa, which invests heavily in gathering Eriocephalus material. Prinias spent 5 of their median 6-day nest construction period adding seed material to their nests and frequently travelled outside their territory boundary to gather Eriocephalus material. Yet, prinias gathered primarily Eriocephalus fluff and actively avoided gathering seeds. The average prinia nest contained only 6.6 seeds, but contained fluff from 579 seeds. These data suggest that prinias provide limited dispersal benefits to Eriocephalus plants. By contrast, the large amounts of Eriocephalus fluff in prinia nests, and the effort that prinias invest in gathering it, suggest that prinias benefit from constructing their nests with Eriocephalus material. We end by outlining hypotheses for possible fitness benefits that Eriocephalus material could provide prinias and other birds. PMID:28280552

  2. Seed storage at elevated partial pressure of oxygen, a fast method for analysing seed ageing under dry conditions

    NARCIS (Netherlands)

    Groot, S.P.C.; Surki, A.A.; Vos, de R.C.H.; Kodde, J.

    2012-01-01

    Background and Aims Despite differences in physiology between dry and relative moist seeds, seed ageing tests most often use a temperature and seed moisture level that are higher than during dry storage used in commercial practice and gene banks. This study aimed to test whether seed ageing under

  3. Incidence of storage fungi and hydropriming on soybean seeds

    OpenAIRE

    Costa,Denis Santiago da; Bonassa,Nathalie; Novembre,Ana Dionisia da Luz Coelho

    2013-01-01

    Priming is a technique applicable to seeds of various plant species; however, for soybean seed there is little available information correlating such technique to the storage fungi. The objective of this study was to assess hydropriming on soybeans seeds and correlate this technique to occurrence of such fungi. For this, soon after acquisition the soybean seeds, cv. M-SOY 7908 RR, were characterized by: moisture content, mechanical damage, viability (seed germination and seedling emergence) a...

  4. Recent approaches for bell pepper seed vigor testing.

    OpenAIRE

    DIAS, M. A. N.; MONDO, V. H. V.; CICERO, S. M.

    2014-01-01

    Seed vigor is a major attribute in the determination of whether a seed lot can be commercialized. Different physiological tests are used to assess seed vigor and are generally based on different concepts, which could lead to variable interpretations, particularly when comparing lots presenting different vigor levels. This study was conducted to compare the different approaches used to evaluate bell pepper seed vigor and verify the applicability of the Seed Vigor Imaging System (SVIS) for this...

  5. Recent approaches for bell pepper seed vigor testing

    OpenAIRE

    Dias, Marcos Altomani Neves; Mondo, Vitor Henrique Vaz; Cicero, Silvio Moure

    2014-01-01

    Seed vigor is a major attribute in the determination of whether a seed lot can be commercialized. Different physiological tests are used to assess seed vigor and are generally based on different concepts, which could lead to variable interpretations, particularly when comparing lots presenting different vigor levels. This study was conducted to compare the different approaches used to evaluate bell pepper seed vigor and verify the applicability of the Seed Vigor Imaging System (SVIS) for this...

  6. Detection of Watermelon Seeds Exterior Quality based on Machine Vision

    OpenAIRE

    Xiai Chen; Ling Wang; Wenquan Chen; Yanfeng Gao

    2013-01-01

    To investigate the detection of watermelon seeds exterior quality, a machine vision system based on least square support vector machine was developed. Appearance characteristics of watermelon seeds included area, perimeter, roughness, minimum enclosing rectangle and solidity were calculated by image analysis after image preprocess.The broken seeds, normal seeds and high-quality seeds were distinguished by least square support vector machine optimized by genetic algorithm. Compared to the grid...

  7. Late Jute seed production in cropland agroforestry system

    OpenAIRE

    Kazi Noor-E-Alam Jewel; Md. Mujibur Rahman; Mohammad Shahjahan; Sayeeduz zaman

    2015-01-01

    Farmers were not self-sufficient in jute seed production and cultivation to avoid use exotic jute seed from different resources. Though the conventional method of jute seed production was not enough to meet the demand of farmers because of shrinkage of jute seed production land. So, late jute seed production technique was applied in agroforestry systems at both established and newly developed orchards. The study was conducted in the selected three sites of Rangpur, Dinajpur and Faridpur. Both...

  8. Investigating Seed Longevity of Big Sagebrush (Artemisia tridentata)

    Science.gov (United States)

    Wijayratne, Upekala C.; Pyke, David A.

    2009-01-01

    The Intermountain West is dominated by big sagebrush communities (Artemisia tridentata subspecies) that provide habitat and forage for wildlife, prevent erosion, and are economically important to recreation and livestock industries. The two most prominent subspecies of big sagebrush in this region are Wyoming big sagebrush (A. t. ssp. wyomingensis) and mountain big sagebrush (A. t. ssp. vaseyana). Increased understanding of seed bank dynamics will assist with sustainable management and persistence of sagebrush communities. For example, mountain big sagebrush may be subjected to shorter fire return intervals and prescribed fire is a tool used often to rejuvenate stands and reduce tree (Juniperus sp. or Pinus sp.) encroachment into these communities. A persistent seed bank for mountain big sagebrush would be advantageous under these circumstances. Laboratory germination trials indicate that seed dormancy in big sagebrush may be habitat-specific, with collections from colder sites being more dormant. Our objective was to investigate seed longevity of both subspecies by evaluating viability of seeds in the field with a seed retrieval experiment and sampling for seeds in situ. We chose six study sites for each subspecies. These sites were dispersed across eastern Oregon, southern Idaho, northwestern Utah, and eastern Nevada. Ninety-six polyester mesh bags, each containing 100 seeds of a subspecies, were placed at each site during November 2006. Seed bags were placed in three locations: (1) at the soil surface above litter, (2) on the soil surface beneath litter, and (3) 3 cm below the soil surface to determine whether dormancy is affected by continued darkness or environmental conditions. Subsets of seeds were examined in April and November in both 2007 and 2008 to determine seed viability dynamics. Seed bank samples were taken at each site, separated into litter and soil fractions, and assessed for number of germinable seeds in a greenhouse. Community composition data

  9. The Seed Semipermeable Layer and Its Relation to Seed Quality Assessment in Four Grass Species

    Directory of Open Access Journals (Sweden)

    Yan Y. Lv

    2017-07-01

    Full Text Available The existence of a semipermeable layer in grass seeds has been extensively reported, yet knowledge of its influence on tests for seed viability and vigor that depend upon measurement of electrical conductivity (EC is limited. This study determined the presence and location of the semipermeable layer, and its relation to seed viability and vigor assessment, in seeds of four important grass species-Elymus nutans Griseb., Lolium perenne L., Leymus chinensis (Trin. Tzvel., and Avena sativa L. Intact seeds of E. nutans, Lolium perenne, and Leymus chinensis exhibited little staining with triphenyl tetrazolium chloride (TTC, and there were no differences in EC between seeds with different germination percentage (GP (P > 0.05. After piercing the seed coat, however, all three species displayed positive staining with TTC, along with a significant negative correlation between EC and GP (E. nutans: R2 = 0.7708; Lolium perenne: R2= 0.8414; Leymus chinensis: R2 = 0.859; P < 0.01. In contrast, both intact and pierced seeds of A. sativa possessed a permeable seed coat that showed positive staining with TTC and EC values that were significantly negatively correlated with GP [R2 = 0.9071 (intact and 0.9597 (pierced; P < 0.01]. In commercial seed lots of A. sativa, a field emergence test indicated that EC showed a significant negative correlation with field emergence at two sowing dates (R2= 0.6069, P < 0.01 and 0.5316, P < 0.05. Analysis of seed coat permeability revealed the presence of a semipermeable layer located in the seed coat adjacent to the endosperm in E. nutans, Lolium perenne, and Leymus chinensis; however, no semipermeable layer was observed in A. sativa. This is the first report of the absence of a semipermeable layer in a grass species. The existence of a semipermeable layer is one of the most important factors affecting seed viability and vigor testing (based on EC measurement in E. nutans, Lolium perenne, and Leymus chinensis. Increasing the

  10. Frugivores bias seed-adult tree associations through nonrandom seed dispersal: a phylogenetic approach.

    Science.gov (United States)

    Razafindratsima, Onja H; Dunham, Amy E

    2016-08-01

    Frugivores are the main seed dispersers in many ecosystems, such that behaviorally driven, nonrandom patterns of seed dispersal are a common process; but patterns are poorly understood. Characterizing these patterns may be essential for understanding spatial organization of fruiting trees and drivers of seed-dispersal limitation in biodiverse forests. To address this, we studied resulting spatial associations between dispersed seeds and adult tree neighbors in a diverse rainforest in Madagascar, using a temporal and phylogenetic approach. Data show that by using fruiting trees as seed-dispersal foci, frugivores bias seed dispersal under conspecific adults and under heterospecific trees that share dispersers and fruiting time with the dispersed species. Frugivore-mediated seed dispersal also resulted in nonrandom phylogenetic associations of dispersed seeds with their nearest adult neighbors, in nine out of the 16 months of our study. However, these nonrandom phylogenetic associations fluctuated unpredictably over time, ranging from clustered to overdispersed. The spatial and phylogenetic template of seed dispersal did not translate to similar patterns of association in adult tree neighborhoods, suggesting the importance of post-dispersal processes in structuring plant communities. Results suggest that frugivore-mediated seed dispersal is important for structuring early stages of plant-plant associations, setting the template for post-dispersal processes that influence ultimate patterns of plant recruitment. Importantly, if biased patterns of dispersal are common in other systems, frugivores may promote tree coexistence in biodiverse forests by limiting the frequency and diversity of heterospecific interactions of seeds they disperse. © 2016 by the Ecological Society of America.

  11. Physical Methods for Seed Invigoration: Advantages and Challenges in Seed Technology.

    Science.gov (United States)

    Araújo, Susana de Sousa; Paparella, Stefania; Dondi, Daniele; Bentivoglio, Antonio; Carbonera, Daniela; Balestrazzi, Alma

    2016-01-01

    In the context of seed technology, the use of physical methods for increasing plant production offers advantages over conventional treatments based on chemical substances. The effects of physical invigoration treatments in seeds can be now addressed at multiple levels, ranging from morpho-structural aspects to changes in gene expression and protein or metabolite accumulation. Among the physical methods available, "magneto-priming" and irradiation with microwaves (MWs) or ionizing radiations (IRs) are the most promising pre-sowing seed treatments. "Magneto-priming" is based on the application of magnetic fields and described as an eco-friendly, cheap, non-invasive technique with proved beneficial effects on seed germination, vigor and crop yield. IRs, as γ-rays and X-rays, have been widely regarded as a powerful tool in agricultural sciences and food technology. Gamma-rays delivered at low dose have showed to enhance germination percentage and seedling establishment, acting as an actual 'priming' treatment. Different biological effects have been observed in seeds subjected to MWs and X-rays but knowledge about their impact as seed invigoration agent or stimulatory effects on germination need to be further extended. Ultraviolet (UV) radiations, namely UV-A and UV-C have shown to stimulate positive impacts on seed health, germination, and seedling vigor. For all mentioned physical treatments, extensive fundamental and applied research is still needed to define the optimal dose, exposition time, genotype- and environment-dependent irradiation conditions. Electron paramagnetic resonance has an enormous potential in seed technology not fully explored to monitor seed invigoration treatments and/or identifying the best suitable irradiation dose or time-point to stop the treatment. The present manuscript describes the use of physical methods for seed invigoration, while providing a critical discussion on the constraints and advantages. The future perspectives related to

  12. Physical Methods for Seed Invigoration: Advantages and Challenges in Seed Technology

    Science.gov (United States)

    Araújo, Susana de Sousa; Paparella, Stefania; Dondi, Daniele; Bentivoglio, Antonio; Carbonera, Daniela; Balestrazzi, Alma

    2016-01-01

    In the context of seed technology, the use of physical methods for increasing plant production offers advantages over conventional treatments based on chemical substances. The effects of physical invigoration treatments in seeds can be now addressed at multiple levels, ranging from morpho-structural aspects to changes in gene expression and protein or metabolite accumulation. Among the physical methods available, “magneto-priming” and irradiation with microwaves (MWs) or ionizing radiations (IRs) are the most promising pre-sowing seed treatments. “Magneto-priming” is based on the application of magnetic fields and described as an eco-friendly, cheap, non-invasive technique with proved beneficial effects on seed germination, vigor and crop yield. IRs, as γ-rays and X-rays, have been widely regarded as a powerful tool in agricultural sciences and food technology. Gamma-rays delivered at low dose have showed to enhance germination percentage and seedling establishment, acting as an actual ‘priming’ treatment. Different biological effects have been observed in seeds subjected to MWs and X-rays but knowledge about their impact as seed invigoration agent or stimulatory effects on germination need to be further extended. Ultraviolet (UV) radiations, namely UV-A and UV-C have shown to stimulate positive impacts on seed health, germination, and seedling vigor. For all mentioned physical treatments, extensive fundamental and applied research is still needed to define the optimal dose, exposition time, genotype- and environment-dependent irradiation conditions. Electron paramagnetic resonance has an enormous potential in seed technology not fully explored to monitor seed invigoration treatments and/or identifying the best suitable irradiation dose or time-point to stop the treatment. The present manuscript describes the use of physical methods for seed invigoration, while providing a critical discussion on the constraints and advantages. The future perspectives

  13. Uncoupling the effects of seed predation and seed dispersal by granivorous ants on plant population dynamics.

    Directory of Open Access Journals (Sweden)

    Xavier Arnan

    Full Text Available Secondary seed dispersal is an important plant-animal interaction, which is central to understanding plant population and community dynamics. Very little information is still available on the effects of dispersal on plant demography and, particularly, for ant-seed dispersal interactions. As many other interactions, seed dispersal by animals involves costs (seed predation and benefits (seed dispersal, the balance of which determines the outcome of the interaction. Separate quantification of each of them is essential in order to understand the effects of this interaction. To address this issue, we have successfully separated and analyzed the costs and benefits of seed dispersal by seed-harvesting ants on the plant population dynamics of three shrub species with different traits. To that aim a stochastic, spatially-explicit individually-based simulation model has been implemented based on actual data sets. The results from our simulation model agree with theoretical models of plant response dependent on seed dispersal, for one plant species, and ant-mediated seed predation, for another one. In these cases, model predictions were close to the observed values at field. Nonetheless, these ecological processes did not affect in anyway a third species, for which the model predictions were far from the observed values. This indicates that the balance between costs and benefits associated to secondary seed dispersal is clearly related to specific traits. This study is one of the first works that analyze tradeoffs of secondary seed dispersal on plant population dynamics, by disentangling the effects of related costs and benefits. We suggest analyzing the effects of interactions on population dynamics as opposed to merely analyzing the partners and their interaction strength.

  14. Physical methods for seed vigourization: advantages and challenges in seed technology

    Directory of Open Access Journals (Sweden)

    Susana eAraújo

    2016-05-01

    Full Text Available In the context of seed technology, the use of physical methods for increasing plant production offers advantages over conventional treatments based on chemical substances. The effects of physical vigourization treatments in seeds can be now addressed at multiple levels, ranging from morpho-structural aspects to changes in gene expression and protein or metabolite accumulation. Among the physical methods available, magneto-priming and irradiation with microwaves or ionizing radiations are the most promissory pre-sowing seed treatments. Magneto-priming is based on the application of magnetic fields and described as an eco-friendly, cheap, non-invasive technique with proved beneficial effects on seed germination, vigour and crop yield. Ionizing radiations, as gamma-rays and X-rays, have been widely regarded as a powerful tool in agricultural sciences and food technology. Gamma-rays delivered at low dose have showed to enhance germination percentage and seedling establishment, acting as an actual ‘priming’ treatment. Different biological effects have been observed in seeds subjected to microwaves and X-rays but knowledge about their impact as seed vigourization agent or stimulatory effects on germination need to be further extended. Ultraviolet (UV radiations, namely UV-A and UV-C have shown to stimulate positive impacts on seed health, germination and seedling vigour. For all mentioned physical treatments, extensive fundamental and applied research is still needed to define the optimal dose, exposition time, genotype- and environment-dependent irradiation conditions. Electron paramagnetic resonance (EPR has an enormous potential in seed technology not fully explored to monitor seed vigourization treatments and/or identifying the best suitable irradiation dose or time-point to stop the treatment. The present manuscript describes the use of physical methods for seed vigourization, while providing a critical discussion on the constraints and

  15. Wheat seed enhancement by vitamin and hormonal priming

    International Nuclear Information System (INIS)

    Khan, M.B.; Gurchani, M.A.; Hussain, M.

    2011-01-01

    Seed priming has proven beneficial in many important agricultural crops. The present study was conducted to explore the role (if any) of hormonal and vitamin seed priming to improve the germination, seedling emergence, early seedling establishment, electrolyte leakage and nutrients uptake in wheat seedlings. The wheat seeds were soaked for 48 hours in aerated solution of salicylic acid, ascorbic acid, kinetin and GA3 with 20 ppm concentration of each solution, whereas untreated seeds were taken as control. Seed priming with ascorbic acid resulted in maximum final germination and emergence percentage (FGP and FEP), radical and plumule length, root and shoot length, number of secondary roots, root shoot ratio, root dry weight, shoot dry weight and seedling dry weight compared to control (untreated seeds). Minimum mean germination and emergence time (MGT and MET) was recorded in seeds primed with kinetin and GA3. While in case of biochemical attributes, seedling potassium contents were decreased by hormonal seed priming while total soluble sugars were increased by salicylic acid and ascorbic acid seed priming. Hormonal seed priming had non-significant effect on phosphorus seedling contents. Untreated (Control) seeds showed maximum electrical conductivity at 0.5, 1, 2, 6, 12 and 24 h after inbibition than primed seeds against the minimum electrical conductivity that was recorded in seeds primed with salicylic acid and ascorbic acid. In conclusion, the wheat seeds primed with 20 ppm solution of ascorbic acid may be used for wheat seed invigoration. (author)

  16. Gene expression analysis of flax seed development

    Science.gov (United States)

    2011-01-01

    Background Flax, Linum usitatissimum L., is an important crop whose seed oil and stem fiber have multiple industrial applications. Flax seeds are also well-known for their nutritional attributes, viz., omega-3 fatty acids in the oil and lignans and mucilage from the seed coat. In spite of the importance of this crop, there are few molecular resources that can be utilized toward improving seed traits. Here, we describe flax embryo and seed development and generation of comprehensive genomic resources for the flax seed. Results We describe a large-scale generation and analysis of expressed sequences in various tissues. Collectively, the 13 libraries we have used provide a broad representation of genes active in developing embryos (globular, heart, torpedo, cotyledon and mature stages) seed coats (globular and torpedo stages) and endosperm (pooled globular to torpedo stages) and genes expressed in flowers, etiolated seedlings, leaves, and stem tissue. A total of 261,272 expressed sequence tags (EST) (GenBank accessions LIBEST_026995 to LIBEST_027011) were generated. These EST libraries included transcription factor genes that are typically expressed at low levels, indicating that the depth is adequate for in silico expression analysis. Assembly of the ESTs resulted in 30,640 unigenes and 82% of these could be identified on the basis of homology to known and hypothetical genes from other plants. When compared with fully sequenced plant genomes, the flax unigenes resembled poplar and castor bean more than grape, sorghum, rice or Arabidopsis. Nearly one-fifth of these (5,152) had no homologs in sequences reported for any organism, suggesting that this category represents genes that are likely unique to flax. Digital analyses revealed gene expression dynamics for the biosynthesis of a number of important seed constituents during seed development. Conclusions We have developed a foundational database of expressed sequences and collection of plasmid clones that comprise

  17. Gene expression analysis of flax seed development

    Directory of Open Access Journals (Sweden)

    Sharpe Andrew

    2011-04-01

    Full Text Available Abstract Background Flax, Linum usitatissimum L., is an important crop whose seed oil and stem fiber have multiple industrial applications. Flax seeds are also well-known for their nutritional attributes, viz., omega-3 fatty acids in the oil and lignans and mucilage from the seed coat. In spite of the importance of this crop, there are few molecular resources that can be utilized toward improving seed traits. Here, we describe flax embryo and seed development and generation of comprehensive genomic resources for the flax seed. Results We describe a large-scale generation and analysis of expressed sequences in various tissues. Collectively, the 13 libraries we have used provide a broad representation of genes active in developing embryos (globular, heart, torpedo, cotyledon and mature stages seed coats (globular and torpedo stages and endosperm (pooled globular to torpedo stages and genes expressed in flowers, etiolated seedlings, leaves, and stem tissue. A total of 261,272 expressed sequence tags (EST (GenBank accessions LIBEST_026995 to LIBEST_027011 were generated. These EST libraries included transcription factor genes that are typically expressed at low levels, indicating that the depth is adequate for in silico expression analysis. Assembly of the ESTs resulted in 30,640 unigenes and 82% of these could be identified on the basis of homology to known and hypothetical genes from other plants. When compared with fully sequenced plant genomes, the flax unigenes resembled poplar and castor bean more than grape, sorghum, rice or Arabidopsis. Nearly one-fifth of these (5,152 had no homologs in sequences reported for any organism, suggesting that this category represents genes that are likely unique to flax. Digital analyses revealed gene expression dynamics for the biosynthesis of a number of important seed constituents during seed development. Conclusions We have developed a foundational database of expressed sequences and collection of plasmid

  18. Incidence of seed migration to the chest, abdomen, and pelvis after transperineal interstitial prostate brachytherapy with loose 125I seeds

    International Nuclear Information System (INIS)

    Sugawara, Akitomo; Shigematsu, Naoyuki; Nakashima, Jun; Kunieda, Etsuo; Nagata, Hirohiko; Mizuno, Ryuichi; Seki, Satoshi; Shiraishi, Yutaka; Kouta, Ryuichi; Oya, Mototsugu

    2011-01-01

    The aim was to determine the incidence of seed migration not only to the chest, but also to the abdomen and pelvis after transperineal interstitial prostate brachytherapy with loose 125 I seeds. We reviewed the records of 267 patients who underwent prostate brachytherapy with loose 125 I seeds. After seed implantation, orthogonal chest radiographs, an abdominal radiograph, and a pelvic radiograph were undertaken routinely to document the occurrence and sites of seed migration. The incidence of seed migration to the chest, abdomen, and pelvis was calculated. All patients who had seed migration to the abdomen and pelvis subsequently underwent a computed tomography scan to identify the exact location of the migrated seeds. Postimplant dosimetric analysis was undertaken, and dosimetric results were compared between patients with and without seed migration. A total of 19,236 seeds were implanted in 267 patients. Overall, 91 of 19,236 (0.47%) seeds migrated in 66 of 267 (24.7%) patients. Sixty-nine (0.36%) seeds migrated to the chest in 54 (20.2%) patients. Seven (0.036%) seeds migrated to the abdomen in six (2.2%) patients. Fifteen (0.078%) seeds migrated to the pelvis in 15 (5.6%) patients. Seed migration occurred predominantly within two weeks after seed implantation. None of the 66 patients had symptoms related to the migrated seeds. Postimplant prostate D90 was not significantly different between patients with and without seed migration. We showed the incidence of seed migration to the chest, abdomen and pelvis. Seed migration did not have a significant effect on postimplant prostate D90

  19. Effects of seed pretreatment and seed source on germination of five ...

    African Journals Online (AJOL)

    The effects of seed pre-sowing treatment and geographic source of seeds on three germination parameters of five Acacia species (GP = germination percent; GMT = germination mean time (days) and GI = germination index) were studied. Pre-sowing treatment included immersion in concentrated sulphuric acid for 5, 10 and ...

  20. Impact of heat stress during seed development on soybean seed metabolome

    Science.gov (United States)

    Seed development is a temperature-sensitive process that is much more vulnerable than vegetative tissues to abiotic stresses. Climate change is expected to increase the incidence and severity of summer heatwaves, and the impact of heat stress on seed development is expected to become more widespread...

  1. Effects of seed predators of different body size on seed mortality in Bornean logged forest.

    Science.gov (United States)

    Hautier, Yann; Saner, Philippe; Philipson, Christopher; Bagchi, Robert; Ong, Robert C; Hector, Andy

    2010-07-19

    The Janzen-Connell hypothesis proposes that seed and seedling enemies play a major role in maintaining high levels of tree diversity in tropical forests. However, human disturbance may alter guilds of seed predators including their body size distribution. These changes have the potential to affect seedling survival in logged forest and may alter forest composition and diversity. We manipulated seed density in plots beneath con- and heterospecific adult trees within a logged forest and excluded vertebrate predators of different body sizes using cages. We show that small and large-bodied predators differed in their effect on con- and heterospecific seedling mortality. In combination small and large-bodied predators dramatically decreased both con- and heterospecific seedling survival. In contrast, when larger-bodied predators were excluded small-bodied predators reduced conspecific seed survival leaving seeds coming from the distant tree of a different species. Our results suggest that seed survival is affected differently by vertebrate predators according to their body size. Therefore, changes in the body size structure of the seed predator community in logged forests may change patterns of seed mortality and potentially affect recruitment and community composition.

  2. Lack of Globulin Synthesis during Seed Development Alters Accumulation of Seed Storage Proteins in Rice

    Directory of Open Access Journals (Sweden)

    Hye-Jung Lee

    2015-06-01

    Full Text Available The major seed storage proteins (SSPs in rice seeds have been classified into three types, glutelins, prolamins, and globulin, and the proportion of each SSP varies. It has been shown in rice mutants that when either glutelins or prolamins are defective, the expression of another type of SSP is promoted to counterbalance the deficit. However, we observed reduced abundances of glutelins and prolamins in dry seeds of a globulin-deficient rice mutant (Glb-RNAi, which was generated with RNA interference (RNAi-induced suppression of globulin expression. The expression of the prolamin and glutelin subfamily genes was reduced in the immature seeds of Glb-RNAi lines compared with those in wild type. A proteomic analysis of Glb-RNAi seeds showed that the reductions in glutelin and prolamin were conserved at the protein level. The decreased pattern in glutelin was also significant in the presence of a reductant, suggesting that the polymerization of the glutelin proteins via intramolecular disulfide bonds could be interrupted in Glb-RNAi seeds. We also observed aberrant and loosely packed structures in the storage organelles of Glb-RNAi seeds, which may be attributable to the reductions in SSPs. In this study, we evaluated the role of rice globulin in seed development, showing that a deficiency in globulin could comprehensively reduce the expression of other SSPs.

  3. Proteome profiling of flax (Linum usitatissimum) seed: characterization of functional metabolic pathways operating during seed development.

    Science.gov (United States)

    Barvkar, Vitthal T; Pardeshi, Varsha C; Kale, Sandip M; Kadoo, Narendra Y; Giri, Ashok P; Gupta, Vidya S

    2012-12-07

    Flax (Linum usitatissimum L.) seeds are an important source of food and feed due to the presence of various health promoting compounds, making it a nutritionally and economically important plant. An in-depth analysis of the proteome of developing flax seed is expected to provide significant information with respect to the regulation and accumulation of such storage compounds. Therefore, a proteomic analysis of seven seed developmental stages (4, 8, 12, 16, 22, 30, and 48 days after anthesis) in a flax variety, NL-97 was carried out using a combination of 1D-SDS-PAGE and LC-MSE methods. A total 1716 proteins were identified and their functional annotation revealed that a majority of them were involved in primary metabolism, protein destination, storage and energy. Three carbon assimilatory pathways appeared to operate in flax seeds. Reverse transcription quantitative PCR of selected 19 genes was carried out to understand their roles during seed development. Besides storage proteins, methionine synthase, RuBisCO and S-adenosylmethionine synthetase were highly expressed transcripts, highlighting their importance in flax seed development. Further, the identified proteins were mapped onto developmental seed specific expressed sequence tag (EST) libraries of flax to obtain transcriptional evidence and 81% of them had detectable expression at the mRNA level. This study provides new insights into the complex seed developmental processes operating in flax.

  4. Distribution of western juniper seeds across an ecotone and implications for seed dispersal processes

    Science.gov (United States)

    Western juniper forests have been the focus of extensive research and management due to range expansion and infilling that began over a century ago. Understanding juniper seed dispersal is vital to identifying processes behind increases in density and range. Dispersal of Juniperus seeds has generall...

  5. Local seed businesses in Uganda: a market-oriented approach towards community seed production

    NARCIS (Netherlands)

    Mastenbroek, A.

    2015-01-01

    The integrated seed sector development (ISSD) programme aims to improve food security and economic development, by providing smallholder farmers with sustainable access to quality seed of superior varieties. The specific objective of the programme is to create a vibrant, pluralistic and

  6. Effect of planting dates on seed yield and seed quality of Stylosanthes guianensis CIAT 184

    Directory of Open Access Journals (Sweden)

    Chureerat Satjipanon

    2005-11-01

    Full Text Available The objective of this experiment was to investigate the effect of planting dates on seed yield and quality of Stylosanthes guianensis CIAT 184 at Khon Kaen Animal Nutrition Research and Development Center, during May 2003 to February 2004. A randomized complete block design with four replications was used. Experimental treatments consisted of four planting dates spaced at about 30-day intervals from 23 May to 23 August 2003.The results revealed that planting date had a significant effect on seed yields and pure germinable seed yields (PGSY of S. guianensis CIAT 184. Plots planted on 23 July produced the highest seed yield and PGSY of 630 and 601 kg/ha, respectively followed by plots planted on 23 June and 23 May (514 and 501; 443 and 421 kg/ha, respectively. Plots planted on 23 August produced the lowest seed yield and PGSY of 269 and 262 kg/ha, respectively. There were no significant differences in seed purity percentage, germination percentage and 1000-seed weight among planting dates. Based on this research, it was concluded that late- July was the optimum planting date for S. guianensis CIAT 184 cultivation for seed production in Northeast Thailand.

  7. Seed size and provenance mediate the joint effects of disturbance and seed predation on community assembly

    Science.gov (United States)

    John L. Maron; Dean E. Pearson; Teal Potter; Yvette K. Ortega

    2012-01-01

    Local plant community assembly is influenced by a series of filters that affect the recruitment and establishment of species. These filters include regional factors that limit seeds of any given species from reaching a local site as well as local interactions such as post-dispersal seed predation and disturbance, which dictate what species actually establish. How these...

  8. Levitation and guidance force relaxations of the single-seeded and multi-seeded YBCO superconductors

    Science.gov (United States)

    Abdioglu, M.; Ozturk, K.; Kabaer, M.; Ekici, M.

    2018-01-01

    The stable levitation and guidance forces at higher force levels are important parameters for technological applicability of high temperature superconductors (HTSs) in Maglev and Flywheel energy storage systems. In this study, we have investigated the levitation and guidance force relaxation of both the single-seeded and multi-seeded YBCOs for different (HTS)-permanent magnetic guideway (PMG) arrangements in different cooling heights (CH). The measured saturated force values of Halbach PMG arrangements are bigger than the maximum force values of other PMGs. It is determined that the normalized magnetic levitation force (MLF) and normalized guidance force (GF) relaxation rate values decrease while the relaxation rates increase with increasing magnetic pole number and the effective external magnetic field area for both the single-seeded and multi-seeded YBCO. Also it can be said that the force stability at the higher force value of Halbach PMG arrangement indicates that the relaxation quality of Halbach PMG is better than that of the others. Additionally, it can be said that both the MLF and GF relaxation qualities of the multi-seeded YBCOs are better than that of the single-seeded ones. This magnetic force and relaxation results of the single-seeded and multi-seeded YBCOs are useful to optimize the loading capacity and lateral reliability of HTS Maglev and similar magnetic bearing systems.

  9. Identification of the infection route of a Fusarium seed pathogen into nondormant Bromus tectorum seeds

    Science.gov (United States)

    JanaLynn Franke; Brad Geary; Susan E. Meyer

    2014-01-01

    The genus Fusarium has a wide host range and causes many different forms of plant disease. These include seed rot and seedling blight diseases of cultivated plants. The diseases caused by Fusarium on wild plants are less well-known. In this study, we examined disease development caused by Fusarium sp. n on nondormant seeds of the important rangeland weed Bromus...

  10. Non- chemical methods of seed treatment for control of seed- borne pathogens on vegetables

    NARCIS (Netherlands)

    Amein, T.; Wright, S.A.I.; Wickstrom, M.; Schmitt, A.; Koch, E.; Wolf, van der J.M.; Groot, S.P.C.; Werner, S.; Jahn, M.

    2006-01-01

    The aim of EU-project "Seed Treatments for Organic Vegetable Production" (STOVE) was to evaluate non-chemical methods for control of seed-borne pathogens in organic vegetable production. Physical (hot air, hot water and electron) and biologi-cal (microorganisms and different agents of natural

  11. Interactions between seed traits and digestive processes determine the germinability of bird-dispersed seeds

    NARCIS (Netherlands)

    Kleyheeg, Erik; Claessens, Mascha; Soons, Merel B.

    2018-01-01

    Waterbirds disperse a wide range of plant seeds via their guts, promoting biotic connectivity between isolated habitat patches. However, the intensity of digestive forces encountered by seeds, and therefore their potential to survive digestive tract passage, varies within and between waterbird

  12. Influence of seed density and aggregation on post-dispersal weed seed predation in cereal fields

    NARCIS (Netherlands)

    Marino, P.C.; Westerman, P.R.; Pinkert, C.; Werf, van der W.

    2005-01-01

    The importance of density dependence, aggregation and background density of seeds on intensity of seed predation in cereal fields were examined in central Netherlands. Four sequential 1-week trials were conducted from 9 July to 8 August 2001 and lamb's quarters (Chenopodium album) was used as the

  13. ECOPHYSIOLOGICAL ANALYSIS OF GENIPA AMERICANA L. SEEDS IN AN INDUCED SOIL SEED BANK

    Directory of Open Access Journals (Sweden)

    Francesca Salla

    2016-03-01

    Full Text Available This work studied the behavior of G. americana L. (Rubiaceae seeds in an induced soil seed bank. This study was conducted in Lavras-MG in a seasonal semideciduous forest of the Federal University of Lavras. Seeds collected in the area were cleaned and analyzed (water content, viability and desiccation tolerance. Soil banks were installed in two distinct areas (top of hill and riparian forest located in the understory of the forest. Seeds were placed in nylon nets and covered with a thin litter layer that was previously removed for this purpose. Monthly assessment of germination, seedling emergence, seed and soil water content, and the characterization of the initial seedling growth was performed. It was observed that G. americana seeds forms a transitory seed bank, with maintenance of its viability until the fourth month after disposal in natural environment. In general, the seed bank located in the riparian forest area showed the best potential for seedling establishment in a natural environment, which supports the ecological behavior of this species.

  14. Changes in seed water status as characterized by NMR in developing soybean seed grown under moisture stress conditions

    International Nuclear Information System (INIS)

    Krishnan, P.; Singh, Ravender; Verma, A.P.S.; Joshi, D.K.; Singh, Sheoraj

    2014-01-01

    Highlights: • In developing soybean seeds, moisture stress resulted in more proportion of water to bound state. • These changes are further corroborated by concomitant changes in seed metabolites. • Thus there exists a moisture stress and development stage dependence of seed tissue water status. - Abstract: Changes in water status of developing seeds of Soybean (Glycine max L. Merrill.) grown under different moisture stress conditions were characterized by proton nuclear magnetic resonance (NMR)- spin–spin relaxation time (T 2 ). A comparison of the seed development characteristics, composition and physical properties indicated that, characteristics like seed weight, seed number/ear, rate of seed filling increased with development stages but decreased with moisture stress conditions. The NMR- spin–spin relaxation (T 2 ) component like bound water increased with seed maturation (40–50%) but decreased with moisture stress conditions (30–40%). The changes in seed water status to increasing levels of moisture stress and seed maturity indicates that moisture stress resulted in more proportion of water to bound state and intermediate state and less proportion of water in free-state. These changes are further corroborated by significant changes in protein and starch contents in seeds under high moisture stress treatments. Thus seed water status during its development is not only affected by development processes but also by moisture stress conditions. This study strongly indicated a clear moisture stress and development stage dependence of seed tissue water status in developing soybean seeds

  15. Changes in seed water status as characterized by NMR in developing soybean seed grown under moisture stress conditions

    Energy Technology Data Exchange (ETDEWEB)

    Krishnan, P., E-mail: pkrishnan@iari.res.in; Singh, Ravender; Verma, A.P.S.; Joshi, D.K.; Singh, Sheoraj

    2014-02-21

    Highlights: • In developing soybean seeds, moisture stress resulted in more proportion of water to bound state. • These changes are further corroborated by concomitant changes in seed metabolites. • Thus there exists a moisture stress and development stage dependence of seed tissue water status. - Abstract: Changes in water status of developing seeds of Soybean (Glycine max L. Merrill.) grown under different moisture stress conditions were characterized by proton nuclear magnetic resonance (NMR)- spin–spin relaxation time (T{sub 2}). A comparison of the seed development characteristics, composition and physical properties indicated that, characteristics like seed weight, seed number/ear, rate of seed filling increased with development stages but decreased with moisture stress conditions. The NMR- spin–spin relaxation (T{sub 2}) component like bound water increased with seed maturation (40–50%) but decreased with moisture stress conditions (30–40%). The changes in seed water status to increasing levels of moisture stress and seed maturity indicates that moisture stress resulted in more proportion of water to bound state and intermediate state and less proportion of water in free-state. These changes are further corroborated by significant changes in protein and starch contents in seeds under high moisture stress treatments. Thus seed water status during its development is not only affected by development processes but also by moisture stress conditions. This study strongly indicated a clear moisture stress and development stage dependence of seed tissue water status in developing soybean seeds.

  16. Peasant seeds in Europe: stakes and prospects

    Directory of Open Access Journals (Sweden)

    Riccardo Bocci

    2011-11-01

    Full Text Available The myth of technological progress in agriculture and then modern plant breeding have resulted in a separation of farming from breeding activities. Seed laws have also contributed to this outcome (e.g. by imposing strict rules for the entry of varieties in the official catalogue. Nowadays, some pioneers, among them farmers rejecting industrialized agriculture and more often practising organic agriculture, are proposing a different option. In Europe, since the beginning of this century, they have been organizing themselves in networks: the Réseau Semences Paysannes in France, the Red de Semillas in Spain and the Rete Semi Rurali in Italy. Their members are farmers, consumers and scientists working together in order to reconsider the scientific, technical and legal aspects of seed production. These new varieties are designated ‘peasant varieties’, a concept that encompasses two main aspects: the seed, the reproductive part of the plant linked to its terroir, and the variety, shaped by history and coevolved with farmers. Scientists working with these networks are developing participatory plant breeding projects, which aim to broaden agrobiodiversity by creating so called new peasant varieties. The basis of these projects comes from old local varieties and landraces conserved in seed banks. In Europe, the legal framework has evolved since the establishment of ‘conservation varieties’ by directive 98/95. The European research project Farm Seed Opportunities is under way to support new seed policies, in the light also of the different national contexts. The debate in Europe now offers a range of seed systems models. It is time to enlarge this debate to southern countries through the sharing of knowledge between farmers of the North and the South, aiming at conserving agrobiodiversity and promoting rural innovation.

  17. The Effect of Method and Germination Paper Substrate on Viability of Eucalyptus Pellita F. Mull Seed

    OpenAIRE

    Yuniarti, Naning; Megawati, Megawati; Leksono, Budi

    2017-01-01

    Improper seed handling of Eucalyptus pellita will reduce seed quality, so as to improve the viability of the seed proper handling techniques are needed. To investigate the seed germination potency as a result of seed handling germination seed tests are needed. The purpose of this study was to examine the effect of germination method and paper substrate on the viability of E. pellita seeds. Seeds used in this study were from seedling seed orchard in South Sumatra, South Kalimantan, and Riau. ...

  18. Effect of seed rate and row spacing in seed production of Festulolium

    DEFF Research Database (Denmark)

    Deleuran, L C; Gislum, R; Boelt, B

    2010-01-01

    -type festulolium, Paulita, and in a fescue-type festulolium, Hykor. The objectives were to examine the influence of row spacing (12, 24, and 36 cm) and seed rate (8, 12, or 16 kg ha-1) on plant establishment, development, and seed yield. Observations of autumn and spring in-row plant densities indicated......Festulolium ( Festulolium) is a cross between the two species fescue (Festuca L.) and ryegrass (Lolium L.) and is a promising forage and seed crop. To stimulate the production of Danish organic festulolium seeds a three-year field experiment was performed from 1999 to 2002 in a ryegrass...... satisfactory plant establishment in all combinations of seed rate and row spacing. The number of reproductive tillers was in the range from 800 to 2200 m-2 in Paulita and from 500 to 1300 m-2 in Hykor. Row spacing had an effect on the number of reproductive tillers and in both cultivars the highest number...

  19. Effects of environmental variation during seed production on seed dormancy and germination.

    Science.gov (United States)

    Penfield, Steven; MacGregor, Dana R

    2017-02-01

    The environment during seed production has major impacts on the behaviour of progeny seeds. It can be shown that for annual plants temperature perception over the whole life history of the mother can affect the germination rate of progeny, and instances have been documented where these affects cross whole generations. Here we discuss the current state of knowledge of signal transduction pathways controlling environmental responses during seed production, focusing both on events that take place in the mother plant and those that occur directly as a result of environmental responses in the developing zygote. We show that seed production environment effects are complex, involving overlapping gene networks active independently in fruit, seed coat, and zygotic tissues that can be deconstructed using careful physiology alongside molecular and genetic experiments. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  20. Seed yield response to N fertilization and potential of proximal sensing in Italian ryegrass seed crops

    DEFF Research Database (Denmark)

    Vleugels, Tim; Rijckaert, Georges; Gislum, René

    2017-01-01

    Italian ryegrass (Lolium multiflorum L.) seed crops are often routinely fertilized with a predetermined amount of nitrogen (N) fertilizer in spring. However, nitrate leaching and increasing N fertilizer prices require rationalized fertilizer applications without compromises in seed yield....... The objectives of this study were (1) to determine the seed yield response to N fertilization, and (2) to evaluate if NDVI values can reliably predict the N status in Italian ryegrass seed crops. During eight years, field trials were conducted with two cultivars ‘Melclips’ and ‘Melquatro’, and seven N strategies...... applied after the forage cut as single or split application: 0, 60, 60 + 30, 90, 90 + 30, 120 and 150 kg N ha−1. NDVI values were obtained with a ‘GreenSeeker’ optical sensor. Maximum seed yield was attained at 141 kg N ha−1 total available N (92 kg N ha−1 fertilized). Higher fertilizations only increased...