Sample records for biomolecules equipment design

  1. Dental operatory design and equipment. (United States)

    Floyd, M


    Improving and expanding the dental services of a practice can involve purchasing new equipment and even modifying or expanding the physical plant. Operatory design is important to the efficiency with which dental procedures can be performed. Equipment purchases to outfit the dental operatory should be made based on the specific needs and functions of a practice.

  2. When biomolecules meet graphene: from molecular level interactions to material design and applications. (United States)

    Li, Dapeng; Zhang, Wensi; Yu, Xiaoqing; Wang, Zhenping; Su, Zhiqiang; Wei, Gang


    Graphene-based materials have attracted increasing attention due to their atomically-thick two-dimensional structures, high conductivity, excellent mechanical properties, and large specific surface areas. The combination of biomolecules with graphene-based materials offers a promising method to fabricate novel graphene-biomolecule hybrid nanomaterials with unique functions in biology, medicine, nanotechnology, and materials science. In this review, we focus on a summarization of the recent studies in functionalizing graphene-based materials using different biomolecules, such as DNA, peptides, proteins, enzymes, carbohydrates, and viruses. The different interactions between graphene and biomolecules at the molecular level are demonstrated and discussed in detail. In addition, the potential applications of the created graphene-biomolecule nanohybrids in drug delivery, cancer treatment, tissue engineering, biosensors, bioimaging, energy materials, and other nanotechnological applications are presented. This review will be helpful to know the modification of graphene with biomolecules, understand the interactions between graphene and biomolecules at the molecular level, and design functional graphene-based nanomaterials with unique properties for various applications.

  3. Design and characterization of nanomaterial-biomolecule conjugates (United States)

    Yim, Tae-Jin

    In the field of nanobiotechnology, nanoscale dimensions result in physical properties that differ from more conventional bulk material state. The integration of nanomaterials with biomolecules has begun to be used for unique physical properties, and for biological specific recognition, thereby leading to novel nanomaterial-biomolecule conjugates. The direction of this dissertation is to develop biocatalytic nanomaterial-biomolecule conjugates and to characterize them. For this, biological catalysts are employed to combine with nanomaterials. Two large parts include functional ization of nanomaterials with biomolecules and assembly of nanomaterials using a biological catalyst. First part of this thesis work is the exploration of the biocatalytic properties of nanomaterial-biomolecule conjugates. Si nanocolumns have higher surface area which leads more amount of biocatalytis immobilization than flat Si wafer with the same projected area. The enhanced activity of soybean peroxidase (SBP) immobilized onto Si nanocolumns as novel nanostructured supports is focused. Next, the catalytic activity of immobilized DNAzyme onto multiwalled carbon nanotubes (MWNTs) is compared to that in solution phase, and multiple turnovers are examined. The relationship between hybridization efficiency and activity is investigated as a function of surface density of DNAzyme on MWNTs. Then, cellular delivery of silica nanoparticle-protein conjugates is visually confirmed and therefore the intracellular function of a protein delivered by silica nanoparticle-protein conjugates is proved. For one example of the intracellular function, stable SBP immobilized onto silica nanoparticles to activate a prodrug is demonstrated. Second part of this thesis work is the formation of nanostructured materials through the enzymatic assembly of single-walled carbon nanotubes (SWNTs). Enzymatic polymerization of a phenol compound is applied to the bridging of two or more SWNTs functionalized with phenol

  4. 21 CFR 58.61 - Equipment design. (United States)


    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Equipment design. 58.61 Section 58.61 Food and... PRACTICE FOR NONCLINICAL LABORATORY STUDIES Equipment § 58.61 Equipment design. Equipment used in the... of appropriate design and adequate capacity to function according to the protocol and shall...

  5. 40 CFR 792.61 - Equipment design. (United States)


    ... 40 Protection of Environment 31 2010-07-01 2010-07-01 true Equipment design. 792.61 Section 792.61...) GOOD LABORATORY PRACTICE STANDARDS Equipment § 792.61 Equipment design. Equipment used in the... of appropriate design and adequate capacity to function according to the protocol and shall...

  6. Hot conditioning equipment conceptual design report

    Energy Technology Data Exchange (ETDEWEB)

    Bradshaw, F.W., Westinghouse Hanford


    This report documents the conceptual design of the Hot Conditioning System Equipment. The Hot conditioning System will consist of two separate designs: the Hot Conditioning System Equipment; and the Hot Conditioning System Annex. The Hot Conditioning System Equipment Design includes the equipment such as ovens, vacuum pumps, inert gas delivery systems, etc.necessary to condition spent nuclear fuel currently in storage in the K Basins of the Hanford Site. The Hot Conditioning System Annex consists of the facility of house the Hot Conditioning System. The Hot Conditioning System will be housed in an annex to the Canister Storage Building. The Hot Conditioning System will consist of pits in the floor which contain ovens in which the spent nuclear will be conditioned prior to interim storage.

  7. Bifunctional chelating agent for the design and development of site specific radiopharmaceuticals and biomolecule conjugation strategy (United States)

    Katti, Kattesh V.; Prabhu, Kandikere R.; Gali, Hariprasad; Pillarsetty, Nagavara Kishore; Volkert, Wynn A.


    There is provided a method of labeling a biomolecule with a transition metal or radiometal in a site specific manner to produce a diagnostic or therapeutic pharmaceutical compound by synthesizing a P.sub.2 N.sub.2 -bifunctional chelating agent intermediate, complexing the intermediate with a radio metal or a transition metal, and covalently linking the resulting metal-complexed bifunctional chelating agent with a biomolecule in a site specific manner. Also provided is a method of synthesizing the --PR.sub.2 containing biomolecules by synthesizing a P.sub.2 N.sub.2 -bifunctional chelating agent intermediate, complexing the intermediate with a radiometal or a transition metal, and covalently linking the resulting radio metal-complexed bifunctional chelating agent with a biomolecule in a site specific manner. There is provided a therapeutic or diagnostic agent comprising a --PR.sub.2 containing biomolecule.

  8. Optimization of biomolecule separation by combining microscale filtration and design-of-experiment methods. (United States)

    Kazemi, Amir S; Kawka, Karina; Latulippe, David R


    There is considerable interest in developing microscale (i.e., high-throughput) methods that enable multiple filtration experiments to be run in parallel with smaller sample amounts and thus reduce the overall required time and associated cost to run the filtration tests. Previous studies to date have focused on simply evaluating the filtration capacity, not the separation performance. In this work, the stirred-well filtration (SWF) method was used in combination with design-of-experiment (DOE) methods to optimize the separation performance for three binary mixtures of bio-molecules: protein-protein, protein-polysaccharide, and protein-DNA. Using the parallel based format of the SWF method, eight constant-flux ultrafiltration experiments were conducted at once to study the effects of stirring conditions, permeate flux, and/or solution conditions (pH, ionic strength). Four separate filtration tests were conducted for each combination of process variables; in total, over 100 separate tests were conducted. The sieving coefficient and selectivity results are presented to match the DOE design format and enable a greater understanding of the effects of the different process variables that were studied. The method described herein can be used to rapidly determine the optimal combination of process factors that give the best separation performance for a range of membrane-based separations applications and thus obviate the need to run a large number of traditional lab-scale tests. Biotechnol. Bioeng. 2016;113: 2131-2139. © 2016 Wiley Periodicals, Inc.

  9. Improving the hygienic design of closed equipment

    DEFF Research Database (Denmark)

    Friis, Alan; Jensen, Bo Boye Busk


    Maintenance of proper hygiene in closed process equipment is in many ways a complex task. The interaction between the physical design and the nature of fluid flow is of main concern. During cleaning the main performance of the flow is to bring cleaning agents in the right doses to all parts of th...... computational fluid dynamics models to be able to predict the cleaning efficiency in especially complex parts of process plants has excellent potentials for desktop improvements and computer pre-validation of the hygienic performance of process plants....

  10. Design of Modular DNA Triangular-Prism Sensor Enabling Ratiometric and Multiplexed Biomolecule Detection on Single Microbead. (United States)

    Liu, Yu; Chen, Qiaoshu; Liu, Jianbo; Yang, Xiaohai; Guo, Qiuping; Li, Li; Liu, Wei; Wang, Kemin


    DNA nanostructures have emerged as powerful and versatile building blocks for the construction of programmable nanoscale structures and functional sensors for biomarker detection, disease diagnostics and therapy. Here we integrated multiple sensing modules into a single DNA 3D nanoarchitecture with a triangular-prism (TP) structure for ratiometric and multiplexed biomolecule detection on single microbead. In our design, the complementary hybridization of three clip sequences formed TP nanoassemblies in which the six single-strand regions in the top and bottom faces act as binding sites for different sensing modules, including an anchor module, reference sequence module and capture sequence module. The multifunctional modular TP nanostructures were thus exploited for ratiometric and multiplexed biomolecule detection on microbeads. Microbead imaging demonstrated that after ratiometric self-calibration analysis, the imaging deviations resulting from uneven fluorescent intensity distribution and differing probe concentrations were greatly reduced. The rigid nanostructure also conferred the TP as a framework for geometric positioning of different capture sequences. The inclusion of multiple targets led to the formation of sandwich hybridization structures that gave a readily detectable optical response at different fluorescent channels and distinct fingerprint-like pattern arrays. This approach allowed us to discriminate multiplexed biomolecule targets in a simple and efficient fashion. In this module-designed strategy, the diversity of the controlled DNA assembly coupled with the geometrically well-defined rigid nanostructures of the TP assembly provides a flexible and reliable biosensing approach that shows great promise for biomedical applications.

  11. Designing the nanoparticle-biomolecule interface for "targeting and therapeutic delivery". (United States)

    Mahon, Eugene; Salvati, Anna; Baldelli Bombelli, Francesca; Lynch, Iseult; Dawson, Kenneth A


    The endogenous transport mechanisms which occur in living organisms have evolved to allow selective transport and processing operate on a scale of tens of nanometers. This presents the possibility of unprecedented access for engineered nanoscale materials to organs and sub-cellular locations, materials which may in principle be targeted to precise locations for diagnostic or therapeutic gain. For this reason, nano-architectures could represent a truly radical departure as delivery agents for drugs, genes and therapies to treat a host of diseases. Thus, for active targeting, unlike the case of small molecular drugs where molecular structure has evolved to promote higher physiochemical affinity to specific sites, one aims to exploit these energy dependant endogenous processes. Many active targeting strategies have been developed, but despite this truly remarkable potential, in applications they have met with mixed success to date. This situation may have more to do with our current understanding and integration of knowledge across disciplines, than any intrinsic limitation on the vision itself. In this review article we suggest that much more fundamental and detailed control of the nanoparticle-biomolecule interface is required for sustained and general success in this field. In the simplest manifestation, pristine nanoparticles in biological fluids act as a scaffold for biomolecules, which adsorb rapidly to the nanoparticles' surface, conferring a new biological identity to the nanoparticles. It is this nanoparticle-biomolecule interface that is 'read' and acted upon by the cellular machinery. Moreover, where targeting moieties are grafted onto nanoparticles, they may not retain their function as a result of poor orientation, and structural or conformational disruption. Further surface adsorption of biomolecules from the surrounding environment i.e. the formation of a biomolecule corona may also obscure specific surface recognition. To transfer the remarkable

  12. Biomolecule-functionalized polymer brushes. (United States)

    Jiang, Hui; Xu, Fu-Jian


    Functional polymer brushes have been utilized extensively for the immobilization of biomolecules, which is of crucial importance for the development of biosensors and biotechnology. Recent progress in polymerization methods, in particular surface-initiated atom transfer radical polymerization (ATRP), has provided a unique means for the design and synthesis of new biomolecule-functionalized polymer brushes. This current review summarizes such recent research activities. The different preparation strategies for biomolecule immobilization through polymer brush spacers are described in detail. The functional groups of the polymer brushes used for biomolecule immobilization include epoxide, carboxylic acid, hydroxyl, aldehyde, and amine groups. The recent research activities indicate that functional polymer brushes become versatile and powerful spacers for immobilization of various biomolecules to maximize their functionalities. This review also demonstrates that surface-initiated ATRP is used more frequently than other polymerization methods in the designs of new biomolecule-functionalized polymer brushes.

  13. Conceptual design report, CEBAF basic experimental equipment

    Energy Technology Data Exchange (ETDEWEB)



    The Continuous Electron Beam Accelerator Facility (CEBAF) will be dedicated to basic research in Nuclear Physics using electrons and photons as projectiles. The accelerator configuration allows three nearly continuous beams to be delivered simultaneously in three experimental halls, which will be equipped with complementary sets of instruments: Hall A--two high resolution magnetic spectrometers; Hall B--a large acceptance magnetic spectrometer; Hall C--a high-momentum, moderate resolution, magnetic spectrometer and a variety of more dedicated instruments. This report contains a short description of the initial complement of experimental equipment to be installed in each of the three halls.

  14. Maintainability design criteria for packaging of spacecraft replaceable electronic equipment. (United States)

    Kappler, J. R.; Folsom, A. B.


    Maintainability must be designed into long-duration spacecraft and equipment to provide the required high probability of mission success with the least cost and weight. The ability to perform repairs quickly and easily in a space environment can be achieved by imposing specific maintainability design criteria on spacecraft equipment design and installation. A study was funded to investigate and define design criteria for electronic equipment that would permit rapid removal and replacement in a space environment. The results of the study are discussed together with subsequent simulated zero-g demonstration tests of a mockup with new concepts for packaging.

  15. Design and Development of Carborne Survey Equipment

    Directory of Open Access Journals (Sweden)



    Full Text Available In most nuclear and radiological emergencies involving the release of radioactive materials to environment, it is important that data on the geographic distribution of potentially hazardous radioactive materials be quickly presented to the authorities. The mobile radiation detection system makes it possible to measure radioactive materials in the environment at random places because of its easy mobility from place to place. The purpose of the work is to develop a mobile radiation detection system to measure gamma exposure rate, radioactive material on the ground and airborne radioactive particulate in the environment quickly. In this work, we have developed a carborne survey equipment system consisting of three gamma ray detectors, one airbome radioactive particulate sampler, a GPS (Global Positioning System, meteorological sensors, a data acquisition system and an operation software. Performance of the carborne survey equipment has been successfully demonstrated to measure iodine and argon release from medical isotope production facility on Serpong in western Java, Indonesia.

  16. Concurrent Design Study of the Petroleum Equipment CAD System

    Institute of Scientific and Technical Information of China (English)


    Military petroleum equipment, as the special device a nd tool for petroleum storage, transportation, filling, chemical analysis, detec tion and maintenance for the army, is an important means and basis of petroleum indemnificatory. To adapt to modern war in high-tech conditions, the petroleum equipment have to develop towards higher reliability, adaptability, maneuverabil ity, standardization, container and cell type with versatile multi-functions. As a new design theory and design process, concur...

  17. Standard guide for general design considerations for hot cell equipment

    CERN Document Server

    American Society for Testing and Materials. Philadelphia


    1.1 Intent: 1.1.1 The intent of this guide is to provide general design and operating considerations for the safe and dependable operation of remotely operated hot cell equipment. Hot cell equipment is hardware used to handle, process, or analyze nuclear or radioactive material in a shielded room. The equipment is placed behind radiation shield walls and cannot be directly accessed by the operators or by maintenance personnel because of the radiation exposure hazards. Therefore, the equipment is operated remotely, either with or without the aid of viewing. 1.1.2 This guide may apply to equipment in other radioactive remotely operated facilities such as suited entry repair areas, canyons or caves, but does not apply to equipment used in commercial power reactors. 1.1.3 This guide does not apply to equipment used in gloveboxes. 1.2 Applicability: 1.2.1 This guide is intended for persons who are tasked with the planning, design, procurement, fabrication, installation, or testing of equipment used in rem...

  18. Modular Design in Treaty Verification Equipment

    Energy Technology Data Exchange (ETDEWEB)

    Macarthur, Duncan Whittemore [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Benz, Jacob [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Tolk, Keith [Milagro Consulting, Albuquerque, NM (United States); Weber, Tom [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)


    It is widely believed that modular design is a good thing. However, there are often few explicit arguments, or even an agreed range of definitions, to back up this belief. In this paper, we examine the potential range of design modularity, the implications of various amounts of modularity, and the advantages and disadvantages of each level of modular construction. We conclude with a comparison of the advantages and disadvantages of each type, as well as discuss many caveats that should be observed to take advantage of the positive features of modularity and minimize the effects of the negative. The tradeoffs described in this paper will be evaluated during the conceptual design to determine what amount of modularity should be included.

  19. The design and manufacture of the catalyst test equipment

    Energy Technology Data Exchange (ETDEWEB)

    Cho, J. H.; Song, I. T. [Korea Atomic Energy Research Institute, Taejeon (Korea)


    The object of this report is to design and manufacture of the catalyst test equipment for removing tritium(H3) included in heavy water for the heavy water reactor. The design conditions of the reactor with the test equipment are summarized as follows 1) Flow rate : 336 l/min. 2) Pressure : 1.15kg/cm{sup 2}. 3) Maximum Temperature : 80 deg C. The test equipment is composed of the water jacket reactor, water equilibrator, heaters, condensers, tanks and pumps. As well as, it is composed of the water, hydrogen, helium, vacuum, emergency operation and control systems. This report will be used important data for the design and manufacture of the equipment for removing tritium. 30 tabs. (Author)

  20. Reliable design of electronic equipment an engineering guide

    CERN Document Server

    Natarajan, Dhanasekharan


    This book explains reliability techniques with examples from electronics design for the benefit of engineers. It presents the application of de-rating, FMEA, overstress analyses and reliability improvement tests for designing reliable electronic equipment. Adequate information is provided for designing computerized reliability database system to support the application of the techniques by designers. Pedantic terms and the associated mathematics of reliability engineering discipline are excluded for the benefit of comprehensiveness and practical applications. This book offers excellent support

  1. Design review report for modifications to RMCS safety class equipment

    Energy Technology Data Exchange (ETDEWEB)

    Corbett, J.E.


    This report documents the completion of the formal design review for modifications to the Rotary Mode Core Sampling (RMCS) safety class equipment. These modifications are intended to support core sampling operations in waste tanks requiring flammable gas controls. The objective of this review was to approve the Engineering Change Notices affecting safety class equipment used in the RMCS system. The conclusion reached by the review committee was that these changes are acceptable.

  2. A source for microhydrated biomolecules

    Energy Technology Data Exchange (ETDEWEB)

    Förstel, M.; Hergenhahn, U., E-mail: [Max-Planck-Institut für Plasmaphysik, Wendelsteinstraße 1, 17491 Greifswald (Germany); Neustetter, M.; Denifl, S. [Institut für Ionenphysik und Angewandte Physik, Technikerstraße 25, 6020 Innsbruck (Austria); Lelievre, F. [Max-Planck-Institut für Plasmaphysik, Wendelsteinstraße 1, 17491 Greifswald (Germany); University Paris-Sud 11, Faculté des Science d’Orsay, 91405 Orsay (France)


    We describe the construction of an apparatus for the production of a molecular jet of microhydrated biomolecules. Our design uses a water reservoir producing water vapour, which then passes through a separate reservoir containing a vapour of a sublimated biomolecule. The mixture coexpands into a molecular beam apparatus through a conical nozzle. Mass spectra showing water-adenin and water-uracil complexes are shown as typical examples. Suitable expansion conditions are reached without the use of an inert carrier gas.

  3. Verification of the thermal design of electronic equipment

    Energy Technology Data Exchange (ETDEWEB)

    Hienonen, R.; Karjalainen, M.; Lankinen, R. [VTT Automation, Espoo (Finland). ProTechno


    The project `Verification of the thermal design of electronic equipment` studied the methodology to be followed in the verification of thermal design of electronic equipment. This project forms part of the `Cool Electronics` research programme funded by TEKES, the Finnish Technology Development Centre. This project was carried out jointly by VTT Automation, Lappeenranta University of Technology, Nokia Research Center and ABB Industry Oy VSD-Technology. The thermal design of electronic equipment has a significant impact on the cost, reliability, tolerance to different environments, selection of components and materials, and ergonomics of the product. This report describes the method for verification of thermal design. It assesses the goals set for thermal design, environmental requirements, technical implementation of the design, thermal simulation and modelling, and design qualification testing and the measurements needed. The verification method covers all packaging levels of electronic equipment from the system level to the electronic component level. The method described in this report can be used as part of the quality system of a corporation. The report includes information about the measurement and test methods needed in the verification process. Some measurement methods for the temperature, flow and pressure of air are described. (orig.) Published in Finnish VTT Julkaisuja 824. 22 refs.

  4. Engineering electrodynamics electric machine, transformer, and power equipment design

    CERN Document Server

    Turowski, Janusz


    Due to a huge concentration of electromagnetic fields and eddy currents, large power equipment and systems are prone to crushing forces, overheating, and overloading. Luckily, power failures due to disturbances like these can be predicted and/or prevented.Based on the success of internationally acclaimed computer programs, such as the authors' own RNM-3D, Engineering Electrodynamics: Electric Machine, Transformer, and Power Equipment Design explains how to implement industry-proven modeling and design techniques to solve complex electromagnetic phenomena. Considering recent progress in magneti

  5. Thermal design and flight validation for laser communicator equipment (United States)

    Meng, Henghui; Geng, Liyin; Tan, Canghai; Li, Guoqiang


    Laser communicator equipment, designed for advanced optical communication, with a large capacity communication, good encryption and lightweight structures, etc., has a wide range of applications. As for the special transmission characteristic of optical communication, laser phase in the transmission path should be accurate, and less thermal deformation for the optical parts is required in the working process, so the laser communicator equipment has a high level requirement for temperature. Large power units cooling, outer two-dimensional rotating units, temperature control for rotating cable, and high temperature stability and equality, bring a challenge for thermal design. Using structure -electric-thermo-optical integration technology, active and passive thermal control methods are adopt in thermal design for laser communicator equipment: heat-conducted plate and heat pipe were adopted for heat transfer of high heat-flux parts, a new passive and active thermal control method to solve cable cryogenic problems, and high precision temperature control methods were applied for key parts. In-orbit data were analyzed, and the results prove the thermal design correct, and bring a way to thermal control for the equipment with high heat flux and running parts.

  6. [Software and hardware for computer equipment designed for psychophysiological examination]. (United States)

    Matveev, E V; Gal'etov, I V; Vasil'ev, A A; Kravchuk, A Iu; Tereshkina, D V


    Principles of designing software and hardware for new computer equipment for psychophysiological examination were elaborated on the basis of theoretical prerequisites and of analysis of the use of equipment manufactured serially for quantitation of indices of the human higher nervous activity (HNA). The hierarchic structuring of software and hardware enabled, through unification of solutions, the development of new modifications of two equipment sets: computer-based unit "Psihomat" KPFK-99 for psychophysiological examination and computer-based unit "STABILOTEST" ST-01 for the evaluation of the central nervous system by the stability parameters in maintaining the vertical posture. The units have new improved functional features and provide the users with new computer technologies for dealing with research and practical tasks related with examination of human HNA parameters in health and pathology.

  7. The Design of Administrative System Based On Portable Intellectual Equipment

    Institute of Scientific and Technical Information of China (English)

    Zeng,Jianhua; Chen,Haisong; Feng,Mingfa; Liang,Zhaofeng


    Regard property management of intelligentized residential area as the application background, we introduce a kind of method for the design of a management system based on portable intelligent equipments. The system is made up of intelligent terminals and personal computers. The intelligent terminals are for data collection and data storage. They communicate with personal computers through high-speed interface. The personal computers communicate with intelligent devices and carry out data analysis and data processing with VB6.0.

  8. Designing Equipment for Use in Gamma Radiation Environments

    Energy Technology Data Exchange (ETDEWEB)

    Vandergriff, K.U.


    High levels of gamma radiation are known to cause degradation in a variety of materials and components. When designing systems to operate in a high radiation environment, special precautions and procedures should be followed. This report (1) outlines steps that should be followed in designing equipment and (2) explains the general effects of radiation on various engineering materials and components. Much information exists in the literature on radiation effects upon materials. However, very little information is available to give the designer a step-by-step process for designing systems that will be subject to high levels of gamma radiation, such as those found in a nuclear fuel reprocessing facility. In this report, many radiation effect references are relied upon to aid in the design of components and systems.

  9. Conference on Manned Systems Design : New Methods and Equipment

    CERN Document Server

    Kraiss, K-F


    This volume contains the proceedings of a conference held in Freiburg, West Germany, September 22-25, 1980, entitled "Manned Systems Design, New Methods and Equipment". The conference was sponsored by the Special Programme Panel on Human Factors of the Scientific Affairs Division of NATO, and supported by Panel VIII, AC/243, on "Human and Biomedical Sciences". Their sponsorship and support are gratefully acknowledged. The contributions in the book are grouped according to the main themes of the conference with special emphasis on analytical approaches, measurement of performance, and simulator design and evaluat ion. The design of manned systems covers many and highly diversified areas. Therefore, a conference under the general title of "Manned Systems Design" is rather ambitious in itself. However, scientists and engineers engaged in the design of manned systems very often are confronted with problems that can be solved only by having several disciplines working together. So it was felt that knowledge about ...

  10. NATO ARI on Ergonomic Data for Equipment Design

    CERN Document Server


    For many years ergonomists and human engineering specialists have made significant contributions to the solution of problems faced in the area of human labour and to the introduction of their research results and field experience into the process of equipment design. However, the rapid increase in complexity of equipment in use as well as in development demonstrates the necessity of broaden­ ing the point of view continuously. The workshop held in Munich from March 22nd to March 26th, 1982, was an excellent opportunity for the participants to discuss their respective interests and their interpretation of needs for future research. The workshop was sponsored by the Human Factors Special Programme Panel of the Scientific Affairs Division of NATO. This sponsorship, together with the helpful assistance rendered by Drs. Bayraktar, Kroemer, and Sanders, is gratefully acknowledged. This volume contains the papers presented during the workshop. All these papers are directly related to the general aim: the ex­ chang...

  11. Improved Design of Hoisting Equipment with Permanent Magne

    Directory of Open Access Journals (Sweden)

    S. S. Stoyanova


    Full Text Available Hoisting equipment for lifting ferromagnetic loads can be designed while using electromagnets and permanent magnets. The second variant finds wider application due to great successes in creation of hard magnetic materials and significantly less electric power consumption. A shortcoming of this design is in the fact that under operational conditions magnetization and demagnetization processes take place in magnetic circuits with various magnetic resistance due to differences in the attracted loads. The proposed design presupposes that load attraction and lowering are controlled without magnetization and demagnetization of the permanent magnet. This effect is achieved by the following way: a strict permanent magnetic flux is directed to a ferromagnetic load or the flux is deviated from it.

  12. [Design, equipment, and management for air conditioning in operating room]. (United States)

    Fuji, Kumiko; Mizuno, Ju


    In order to maintain air cleanliness in the operating room (OR) permanently, air exchange rate in the OR should be more than 15 times x hr(-1), the laminar air flow should be kept, and the numbers of the persons in the OR and the numbers of opening and closing OR door should be limited. High efficiency particulate air (HEPA) filter is effective in collection and removal of airborne microbes, and is used in the biological clean room. We need to design, equip, and manage the OR environment according to Guideline for Design and Operation of Hospital HVAC Systems HEAS-02-2004 established by Healthcare Engineering Association of Japan and Guideline for Prevention of Surgical Site Infection (SSI) established by the Center for Disease Control and Prevention (CDC) in the USA.

  13. Method for detecting biomolecules (United States)

    Huo, Qisheng; Liu, Jun


    A method for detecting and measuring the concentration of biomolecules in solution, utilizing a conducting electrode in contact with a solution containing target biomolecules, with a film with controllable pore size distribution characteristics applied to at least one surface of the conducting electrode. The film is functionalized with probe molecules that chemically interact with the target biomolecules at the film surface, blocking indicator molecules present in solution from diffusing from the solution to the electrode, thereby changing the electrochemical response of the electrode

  14. Design of tension control system for fiber placement equipments

    Directory of Open Access Journals (Sweden)

    Hongjie CHANG


    Full Text Available Aiming at the tension control requirements of automatic fiber placement equipments and the problems of traditional tension control system, the hammer tension control system is designed. The hammer is used as a buffer unit in the system, and the tension can be indirectly controlled by the position of the hammer. The system adopts the motion controller as the core element of the system, the AC servo motor as the actuator and the laser position sensor as the detector. The fuzzy PID control algorithm is used for the position control of hammer, which can avoid tension fluctuation by full consideration of the acceleration of the hammer. Prototype experimental results show that this system can avoid the problems of disconnection and relaxation of fiber, meanwhile, the static difference ratio and fluctuation ratio can meet the requirements of engineering practice.

  15. Design and Implementation of Equipment Interactive Electronic Technical Manuals

    Institute of Scientific and Technical Information of China (English)


    Interactive electronic technical manuals (IETMs) can significantly improve the maintenance and logistics of modern weapon equipment. Main functiors and development strategies of IETMs are discussed. As an example, the IETM development of a certain equipment is demonstrated.

  16. Design of a molecular imprinting biosensor with multi-scale roughness for detection across a broad spectrum of biomolecules. (United States)

    Yu, Yingjie; Zhang, Qi; Chang, Chung-Chueh; Liu, Ying; Yang, Zhenhua; Guo, Yichen; Wang, Yantian; Galanakis, Dennis K; Levon, Kalle; Rafailovich, Miriam


    The molecular imprinting technique has tremendous applications in artificial enzymes, bioseparation, and sensor devices. In this study, a novel molecular imprinting (MI) biosensor platform was developed for the detection of a broad range of biomolecules with different sizes. Previously this method has been applied to 2D molecular imprinting, where the height of the self-assembled monolayer (SAM) of around 2 nm limited the maximum dimensions of the molecule that can be imprinted to create template-shaped cavities. In order to match the size of the imprinted molecules with the height of the SAM, we propose a model for 3D molecular imprinting where the analyte is sequestered within a niche created by the surface roughness. The SAM is assembled on the walls of the niche, forming a 3D pattern of the analyte uniquely molded to its contour. Surfaces with multi-scale roughness were prepared by evaporation of gold onto electropolished (smooth) and unpolished (rough) Si wafers, where the native roughness was found to have a normal distribution centered around 5 and 90 nm respectively. Our studies using molecules with size ranging on a nanometer scale, from proteins of a few nanometers to bacteria of hundreds of nanometers, showed that when the size of the analyte matched the roughness range of the gold surface, the molecular imprinting process was optimized for the best biosensing performance. After optimization, the MI biosensor platform enabled the identification and quantification of a broad range of biomolecules with great discrimination abilities. Hemoglobin under different pH values and several mutated fibrinogen molecules can also be well differentiated through the test.


    Directory of Open Access Journals (Sweden)

    M. Y. Afanasiev


    Full Text Available This paper considers the question of an optical unit designing for transmitting power laser radiation through an optical fiber. The aim of this work is designing a simple construction unit with minimized reflection losses. The source of radiation in the optical unit described below is an ultraviolet laser with diode pumping. We present the general functioning scheme and designing features for the three main parts: laser beam deflecting system, laser beam dump and optical unit control system. The described laser beam deflection system is composed of a moving flat mirror and a spherical scattering mirror. Comparative analysis of the production technology for such mirrors was carried out, and, as a result, the decision was made to produce both mirrors of 99.99 % pure molybdenum without coating. A moving mirror deflects laser emission from a source through a fiber or deflects it on a spherical mirror and into the laser beam dump, moreover, switching from one position to another occurs almost immediately. It is shown that a scattering mirror is necessary, otherwise, the absorbing surface of the beam dump is being worn out irregularly. The laser beam dump is an open conical cavity, in which the conical element with its spire turned to the emission source is placed. Special microgeometry of the internal surface of the beam dump is suggested for the better absorption effect. An optical unit control system consists of a laser beam deflection system, laser temperature sensor, deflection system solenoid temperature sensor, and deflection mirror position sensor. The signal processing algorithm for signals coming from the sensors to the controller is described. The optical unit will be used in special technological equipment.

  18. Biomolecules in Astrobiology


    Meringer, Markus


    Astrobiology is the study of the origin, distribution and future of life in the universe, biomolecules are molecules produced by living organisms. This talk reviews known facts and open questions about biomolecules in the context of Astrobiology and introduces a research project on "Creating a Reference Set of Amino Acids Structures for Use in Multiple Astrobiology Investigations" that tries to find answers using computational methods.

  19. 78 FR 13911 - Proposed Revision to Design of Structures, Components, Equipment and Systems (United States)


    ..., ``Concrete And Steel Internal Structures of Steel Or Concrete Containments, ``Section 3.8.4, ``Other Seismic... COMMISSION Proposed Revision to Design of Structures, Components, Equipment and Systems AGENCY: Nuclear..., ``Design of Structures, Components, Equipment, and Systems'' and is soliciting public comment on...

  20. 46 CFR 130.220 - Design of equipment for cooking and heating. (United States)


    ... 46 Shipping 4 2010-10-01 2010-10-01 false Design of equipment for cooking and heating. 130.220 Section 130.220 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS... Design of equipment for cooking and heating. (a) Doors on each cooking appliance must be provided...

  1. [Design of a testing system for antithrombotic pressure circulatory equipment]. (United States)

    Zhou, Xun; Cui, Haipo; Shang, Kun; Li, Zhelong; Cheng, Enqing


    A new type of testing system used for antithrombotic pressure circulatory equipment has been developed, which realized a new method for the calibration of pressure sensor. Multi-path control and acquisition functions are achieved by this method based on human-computer interaction testing system. The precision of pressure sensor is ob tained by polynomial fitting for each test point using linear interpolation method. The result showed that the precision test of pressure sensor could be realized easily and efficiently, using the developed testing system, and the parameters of pressure sensor could be calibrated effectively, so that it could be accurately used in the antithrombotic pressure circulatory equipment. The developed testing system has a prosperous future in the aspects of promotion and application.

  2. Frustration in biomolecules. (United States)

    Ferreiro, Diego U; Komives, Elizabeth A; Wolynes, Peter G


    Biomolecules are the prime information processing elements of living matter. Most of these inanimate systems are polymers that compute their own structures and dynamics using as input seemingly random character strings of their sequence, following which they coalesce and perform integrated cellular functions. In large computational systems with finite interaction-codes, the appearance of conflicting goals is inevitable. Simple conflicting forces can lead to quite complex structures and behaviors, leading to the concept of frustration in condensed matter. We present here some basic ideas about frustration in biomolecules and how the frustration concept leads to a better appreciation of many aspects of the architecture of biomolecules, and especially how biomolecular structure connects to function by means of localized frustration. These ideas are simultaneously both seductively simple and perilously subtle to grasp completely. The energy landscape theory of protein folding provides a framework for quantifying frustration in large systems and has been implemented at many levels of description. We first review the notion of frustration from the areas of abstract logic and its uses in simple condensed matter systems. We discuss then how the frustration concept applies specifically to heteropolymers, testing folding landscape theory in computer simulations of protein models and in experimentally accessible systems. Studying the aspects of frustration averaged over many proteins provides ways to infer energy functions useful for reliable structure prediction. We discuss how frustration affects folding mechanisms. We review here how the biological functions of proteins are related to subtle local physical frustration effects and how frustration influences the appearance of metastable states, the nature of binding processes, catalysis and allosteric transitions. In this review, we also emphasize that frustration, far from being always a bad thing, is an essential feature

  3. A "Reverse-Schur" Approach to Optimization With Linear PDE Constraints: Application to Biomolecule Analysis and Design. (United States)

    Bardhan, Jaydeep P; Altman, Michael D; Tidor, B; White, Jacob K


    We present a partial-differential-equation (PDE)-constrained approach for optimizing a molecule's electrostatic interactions with a target molecule. The approach, which we call reverse-Schur co-optimization, can be more than two orders of magnitude faster than the traditional approach to electrostatic optimization. The efficiency of the co-optimization approach may enhance the value of electrostatic optimization for ligand-design efforts-in such projects, it is often desirable to screen many candidate ligands for their viability, and the optimization of electrostatic interactions can improve ligand binding affinity and specificity. The theoretical basis for electrostatic optimization derives from linear-response theory, most commonly continuum models, and simple assumptions about molecular binding processes. Although the theory has been used successfully to study a wide variety of molecular binding events, its implications have not yet been fully explored, in part due to the computational expense associated with the optimization. The co-optimization algorithm achieves improved performance by solving the optimization and electrostatic simulation problems simultaneously, and is applicable to both unconstrained and constrained optimization problems. Reverse-Schur co-optimization resembles other well-known techniques for solving optimization problems with PDE constraints. Model problems as well as realistic examples validate the reverse-Schur method, and demonstrate that our technique and alternative PDE-constrained methods scale very favorably compared to the standard approach. Regularization, which ordinarily requires an explicit representation of the objective function, can be included using an approximate Hessian calculated using the new BIBEE/P (boundary-integral-based electrostatics estimation by preconditioning) method.

  4. Supporting industrial equipment development through a set of design-for-maintenance guidelines

    NARCIS (Netherlands)

    Mulder, W.; Basten, R.J.I.; Jauregui Becker, J.M.; Blok, J.; Hoekstra, S.; Kokkeler, F.G.M.; Marjanovic, Dorian; Storga, Mario; Pavkovic, Neven; Bojcetic, Nenad


    This paper presents a leaflet with design-for-maintenance guidelines. It aims at supporting developers of industrial equipment in their design-for-maintenance practices. The use of this leaflet should lead to increased attention for design-for-maintenance aspects and to improve idea generation. The

  5. Design of highwall mining equipment electronic guidance package

    Energy Technology Data Exchange (ETDEWEB)

    Brenden, B.B.; Morgen, G.P.; Shorpik, J.R.; Farrar, R.


    This report provides design specifics and guidance software for a guidance system intended for use in guiding an auger type highwall mining head through thin coal seams to depths of up to 600 ft. A small desk top computer is used at the operator's station to send and receive signals in serialized bit streams to a downhole microprocessor. The guidance system accepts signals from a variety of sensors and uses these signals to plot and control the course of the auger head automatically using computer algorithms designed to keep the auger head in the coal seam and maintain a specified stump thickness.

  6. Computational On-Chip Imaging of Nanoparticles and Biomolecules using Ultraviolet Light (United States)

    Daloglu, Mustafa Ugur; Ray, Aniruddha; Gorocs, Zoltan; Xiong, Matthew; Malik, Ravinder; Bitan, Gal; McLeod, Euan; Ozcan, Aydogan


    Significant progress in characterization of nanoparticles and biomolecules was enabled by the development of advanced imaging equipment with extreme spatial-resolution and sensitivity. To perform some of these analyses outside of well-resourced laboratories, it is necessary to create robust and cost-effective alternatives to existing high-end laboratory-bound imaging and sensing equipment. Towards this aim, we have designed a holographic on-chip microscope operating at an ultraviolet illumination wavelength (UV) of 266 nm. The increased forward scattering from nanoscale objects at this short wavelength has enabled us to detect individual sub-30 nm nanoparticles over a large field-of-view of >16 mm2 using an on-chip imaging platform, where the sample is placed at ≤0.5 mm away from the active area of an opto-electronic sensor-array, without any lenses in between. The strong absorption of this UV wavelength by biomolecules including nucleic acids and proteins has further enabled high-contrast imaging of nanoscopic aggregates of biomolecules, e.g., of enzyme Cu/Zn-superoxide dismutase, abnormal aggregation of which is linked to amyotrophic lateral sclerosis (ALS) - a fatal neurodegenerative disease. This UV-based wide-field computational imaging platform could be valuable for numerous applications in biomedical sciences and environmental monitoring, including disease diagnostics, viral load measurements as well as air- and water-quality assessment.

  7. Computational On-Chip Imaging of Nanoparticles and Biomolecules using Ultraviolet Light. (United States)

    Daloglu, Mustafa Ugur; Ray, Aniruddha; Gorocs, Zoltan; Xiong, Matthew; Malik, Ravinder; Bitan, Gal; McLeod, Euan; Ozcan, Aydogan


    Significant progress in characterization of nanoparticles and biomolecules was enabled by the development of advanced imaging equipment with extreme spatial-resolution and sensitivity. To perform some of these analyses outside of well-resourced laboratories, it is necessary to create robust and cost-effective alternatives to existing high-end laboratory-bound imaging and sensing equipment. Towards this aim, we have designed a holographic on-chip microscope operating at an ultraviolet illumination wavelength (UV) of 266 nm. The increased forward scattering from nanoscale objects at this short wavelength has enabled us to detect individual sub-30 nm nanoparticles over a large field-of-view of >16 mm(2) using an on-chip imaging platform, where the sample is placed at ≤0.5 mm away from the active area of an opto-electronic sensor-array, without any lenses in between. The strong absorption of this UV wavelength by biomolecules including nucleic acids and proteins has further enabled high-contrast imaging of nanoscopic aggregates of biomolecules, e.g., of enzyme Cu/Zn-superoxide dismutase, abnormal aggregation of which is linked to amyotrophic lateral sclerosis (ALS) - a fatal neurodegenerative disease. This UV-based wide-field computational imaging platform could be valuable for numerous applications in biomedical sciences and environmental monitoring, including disease diagnostics, viral load measurements as well as air- and water-quality assessment.

  8. Design and Development of Decision Support System for Equipping Farm Machines

    Directory of Open Access Journals (Sweden)

    Ying Wang


    Full Text Available Equipping farm machines is the key link of agricultural production process. The decision support system of equipping farm machines is able to aid managers to make scientific and effective decision. In this paper, the decision support system of equipping farm machines is designed and developed based on the related theories and the thought of prototype. The system chooses Delphi 7.0 as development language, and uses three classic equipping methods to establish system models. For the complex linear programming model, firstly it is established by M-file of Matlab, then COM components are generated; finally Delphi calls the COM components to solve. The database of the system is established and managed by SQL Server 2005. It can be seen from the result of the system application study that the system could assist users to equip farm machines more scientifically and dynamically

  9. Improvements of seismic design of nuclear power plant equipment

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, Kohei [Tokyo Metropolitan Univ., Hachioji (Japan). Faculty of Technology; Takayama, Yoshihiro


    A brief survey and overview of the current research and development in Japan was presented. Particularly, several kinds of new dampers and isolators were developed and those effectiveness were examined by caring out the large-scale vibration test and so on. The evaluation of the energy absorption of these damping devices at the earthquake appeared to be significant. In addition, it must be necessary to investigate the design margin and the failure mode and limit problem to these devices and the nuclear structures and piping supported by those. Mutual exchange of the information related to these technology and research has to be put forward and cooperative works including the international conference on those issues should be promoted. (J.P.N.)

  10. Design Report of Volume Reduction and Handling Equipment of ACPF Wastes

    Energy Technology Data Exchange (ETDEWEB)

    Kwon, K. C.; Ku, J. H.; Lee, W. K.; Lee, E. P.; Choung, W. M.; Cho, I. J.; Kook, D. H.; You, G. S.; Youn, J. S


    For the efficient disposal of wastes from the ACPF (Advanced spent fuel Conditioning Process Facility), hot cell filter press equipment, vacuum press equipment of compatible wastes and hot cell auxiliary crane were developed. The filter press equipment installed in hot cell is pneumatically driven and remotely operated. In order to avoid the contamination of hot cell by dust particles from filters being compressed, it is designed to compress filters with them put into the basket of the solid wastes cask. The performance test showed that the height of a filter was reduced by more than 75% and a basket could contain up to four compressed filters. The vacuum press equipment reduces the volume of the plastic bag containing compatible wastes such as cloth, plastic film and paper by vacuumizing the bag. It consists of the vacuum pump, the suction and HEPA filters and the manual pneumatic valve, and is designed to be easily handled and managed. Through the performance test, it is observed that the volume of wastes was reduced to a third. Also an additional crane of 300 kg capacity was manufactured and installed in the ACP hot cell to handle the salt ingot wastes container located where the overhead crane is not accessible. This report presenting the details of the design, manufacturing and performance of these equipment will be used as technical materials for the operation of the facility and the development of equipment.

  11. Seismic design of equipment and piping systems for nuclear power plants in Japan

    Energy Technology Data Exchange (ETDEWEB)

    Minematsu, Akiyoshi [Tokyo Electric Power Co., Inc. (Japan)


    The philosophy of seismic design for nuclear power plant facilities in Japan is based on `Examination Guide for Seismic Design of Nuclear Power Reactor Facilities: Nuclear Power Safety Committee, July 20, 1981` (referred to as `Examination Guide` hereinafter) and the present design criteria have been established based on the survey of governmental improvement and standardization program. The detailed design implementation procedure is further described in `Technical Guidelines for Aseismic Design of Nuclear Power Plants, JEAG4601-1987: Japan Electric Association`. This report describes the principles and design procedure of the seismic design of equipment/piping systems for nuclear power plant in Japan. (J.P.N.)

  12. Supporting industrial equipment development through a set of design-for-maintenance guidelines


    W. Mulder; Basten, R.J.I.; Jauregui Becker, J.M.; Blok, J.; Hoekstra, S.; Kokkeler, F.G.M.; Marjanovic, Dorian; Storga, Mario; Pavkovic, Neven; Bojcetic, Nenad


    This paper presents a leaflet with design-for-maintenance guidelines. It aims at supporting developers of industrial equipment in their design-for-maintenance practices. The use of this leaflet should lead to increased attention for design-for-maintenance aspects and to improve idea generation. The way of presenting the guidelines to the user should ensure that the support will be adopted in practice. The feedback that we have received from industrial partners indicates that the support is us...

  13. Design of a Prognostics and Health Management System for Electromechanical Equipment Through Time Stress Analysis

    Institute of Scientific and Technical Information of China (English)

    L(U) Ke-hong; QIU Jing; LIU Guan-jun


    Time stress includes all kinds of environment and operating stress such as shock, vibration, temperature and electric current that the electromechanical system suffers in the manufacture, transport and operating process. In this paper, the conception of time stress and prognostics and health management (PHM) system are introduced. Then, in order to improve the false alarm recognition and fault prediction capabilities of the electromechanical equipment, a novel PHM architecture for electromechanical equipment is put forward based on a built-in test (BIT) system design technology and time stress analysis method. Finally, the structure, the design and implementing method and the functions of each module of this PHM system are described in detail.

  14. Power system engineering planning, design, and operation of power systems and equipment

    CERN Document Server

    Schlabbach, Juergen


    With its focus on the requirements and procedures of tendering and project contracting, this book enables the reader to adapt the basics of power systems and equipment design to special tasks and engineering projects, e.g. the integration of renewable energy sources.

  15. On Syllabus Design for the Specialty of Electromechanical Equipment Maintenance and Management in Vocational College

    Institute of Scientific and Technical Information of China (English)


    We carried out researches on the syllabus design for the specialty of electromechanical equipment maintenance & management in vocational college. Our researches involve the following aspects: (1) The training objective of the specialty; (2) the curriculum system; (3) The teaching methodology; and (4) Field training.

  16. 78 FR 15755 - Proposed Revision to Design of Structures, Components, Equipment and Systems; Correction (United States)


    ... of New Reactors, U.S. Nuclear Regulatory Commission, Washington, DC 20555- 0001; telephone: 301-415... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Proposed Revision to Design of Structures, Components, Equipment and Systems; Correction...

  17. 78 FR 48727 - Proposed Revisions to Design of Structures, Components, Equipment and Systems (United States)


    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Proposed Revisions to Design of Structures, Components, Equipment and Systems AGENCY: Nuclear.... FOR FURTHER INFORMATION CONTACT: Jonathan DeGange, Office of New Reactors, U.S. Nuclear...

  18. Design and Fabrication of Remote Welding Equipment in a Hot-Cell

    Directory of Open Access Journals (Sweden)

    Soosung Kim


    Full Text Available The remote welding equipment for nuclear fuel bundle fabrication in a hot-cell was designed and developed. To achieve this, a preliminary investigation of hands-on fuel fabrication outside a hot-cell was conducted with a consideration of the constraints caused by the welding in a hot-cell. Some basic experiments were also carried out to improve the end-plate welding process for nuclear fuel bundle fabrication. The resistance welding equipment using end-plate welding was also improved. It was found that the remote resistance welding was more suitable for joining an end-plate to end caps in a hot-cell. This paper presents an outline of the developed welding equipment for nuclear fuel bundle fabrication and reviews a conceptual design of remote welding equipment using a master-slave manipulator. Furthermore, the mechanical considerations and a mock-up simulation test were described. Finally, its performance test results were presented for a mock-up of the remote resistance welding equipment for nuclear fuel bundle fabrication.

  19. Improvement of Productivity in TIG Welding Plant by Equipment Design in Orbit (United States)

    Gnanavel, C.; Saravanan, R.; Chandrasekaran, M.; Jayakanth, J. J.


    Measurements and improvements are very indispensable task at all levels of management. Here some samples are, at operator level: Measuring operating parameters to ensure OEE (Overall Equipment Effectiveness) and measuring Q components performance to ensure quality, at supervisory level: measuring operator’s performance to ensure labour utility at managerial level: production and productivity measurements and at top level capital and capacity utilization. An often accepted statement is “Improvement is impossible without measurement”. Measurements often referred as observation. The case study was conducted at Government Boiler factory in India. The scientific approach followed for indentifying non value added activities. Personalised new equipment designed and installed to achieve productivity improvement of 85% for a day. The new equipment can serve 360o around its axis hence it simplified loading and unloading procedures as well as reduce their times and ensured effective space and time.

  20. Design of a compact low-power human-computer interaction equipment for hand motion (United States)

    Wu, Xianwei; Jin, Wenguang


    Human-Computer Interaction (HCI) raises demand of convenience, endurance, responsiveness and naturalness. This paper describes a design of a compact wearable low-power HCI equipment applied to gesture recognition. System combines multi-mode sense signals: the vision sense signal and the motion sense signal, and the equipment is equipped with the depth camera and the motion sensor. The dimension (40 mm × 30 mm) and structure is compact and portable after tight integration. System is built on a module layered framework, which contributes to real-time collection (60 fps), process and transmission via synchronous confusion with asynchronous concurrent collection and wireless Blue 4.0 transmission. To minimize equipment's energy consumption, system makes use of low-power components, managing peripheral state dynamically, switching into idle mode intelligently, pulse-width modulation (PWM) of the NIR LEDs of the depth camera and algorithm optimization by the motion sensor. To test this equipment's function and performance, a gesture recognition algorithm is applied to system. As the result presents, general energy consumption could be as low as 0.5 W.

  1. Biomolecule immobilization techniques for bioactive paper fabrication. (United States)

    Kong, Fanzhi; Hu, Yim Fun


    Research into paper-based sensors or functional materials that can perform analytical functions with active recognition capabilities is rapidly expanding, and significant research effort has been made into the design and fabrication of bioactive paper at the biosensor level to detect potential health hazards. A key step in the fabrication of bioactive paper is the design of the experimental and operational procedures for the immobilization of biomolecules such as antibodies, enzymes, phages, cells, proteins, synthetic polymers and DNA aptamers on a suitably prepared paper membrane. The immobilization methods are concisely categorized into physical absorption, bioactive ink entrapment, bioaffinity attachment and covalent chemical bonding immobilization. Each method has individual immobilization characteristics. Although every biomolecule-paper combination has to be optimized before use, the bioactive ink entrapment method is the most commonly used approach owing to its general applicability and biocompatibility. Currently, there are four common applications of bioactive paper: (1) paper-based bioassay or paper-based analytical devices for sample conditioning; (2) counterfeiting and countertempering in the packaging and construction industries; (3) pathogen detection for food and water quality monitoring; and (4) deactivation of pathogenic bacteria using antimicrobial paper. This article reviews and compares the different biomolecule immobilization techniques and discusses current trends. Current, emerging and future applications of bioactive paper are also discussed.

  2. Diffusion phenomena of cells and biomolecules in microfluidic devices. (United States)

    Yildiz-Ozturk, Ece; Yesil-Celiktas, Ozlem


    Biomicrofluidics is an emerging field at the cross roads of microfluidics and life sciences which requires intensive research efforts in terms of introducing appropriate designs, production techniques, and analysis. The ultimate goal is to deliver innovative and cost-effective microfluidic devices to biotech, biomedical, and pharmaceutical industries. Therefore, creating an in-depth understanding of the transport phenomena of cells and biomolecules becomes vital and concurrently poses significant challenges. The present article outlines the recent advancements in diffusion phenomena of cells and biomolecules by highlighting transport principles from an engineering perspective, cell responses in microfluidic devices with emphases on diffusion- and flow-based microfluidic gradient platforms, macroscopic and microscopic approaches for investigating the diffusion phenomena of biomolecules, microfluidic platforms for the delivery of these molecules, as well as the state of the art in biological applications of mammalian cell responses and diffusion of biomolecules.

  3. 48 CFR 52.246-19 - Warranty of Systems and Equipment under Performance Specifications or Design Criteria. (United States)


    ... Equipment under Performance Specifications or Design Criteria. 52.246-19 Section 52.246-19 Federal... under Performance Specifications or Design Criteria. As prescribed in 46.710(c)(1), the contracting officer may insert a clause substantially as follows: Warranty of Systems and Equipment Under...

  4. Equipment Design and Cost Estimation for Small Modular Biomass Systems, Synthesis Gas Cleanup, and Oxygen Separation Equipment; Task 2.3: Sulfur Primer

    Energy Technology Data Exchange (ETDEWEB)

    Nexant Inc.


    This deliverable is Subtask 2.3 of Task 2, Gas Cleanup Design and Cost Estimates, of NREL Award ACO-5-44027, ''Equipment Design and Cost Estimation for Small Modular Biomass Systems, Synthesis Gas Cleanup and Oxygen Separation Equipment''. Subtask 2.3 builds upon the sulfur removal information first presented in Subtask 2.1, Gas Cleanup Technologies for Biomass Gasification by adding additional information on the commercial applications, manufacturers, environmental footprint, and technical specifications for sulfur removal technologies. The data was obtained from Nexant's experience, input from GTI and other vendors, past and current facility data, and existing literature.

  5. Design and implementation of equipment for monitoring the salinity in the subsoil

    Directory of Open Access Journals (Sweden)

    Mariano Norzagaray Campos


    Full Text Available Geochemical exploration equipments to explore the contaminants and structures geological in the subsoil come from abroad are expensive and sometimes parts for replacement are not available in the market. So it is necessary design apparatus that meet cover these needs. To the monitoring of variations in salinity there is semi-automatic equipment, but it always has difficult to manage. However, is not equipment for the indirect study of salinity in the subsoil. In this work was design equipment for measurement the apparent resistivity in the subsoil, at same time allow know the salinity, as well as the detection of any pollutant in groundwater. For make it, was selected a design of earthing systems, with electronic hardware which were jointed for apply to subsoil a direct current (DC through an array dipole-dipole and vertical electric sounding, with brass and stainless steel electrodes. In the earthing systems the electrodes were collocated in the equidistant line between the detectors of potential and current. A geometric factor (K, that depend on theelectrodes distance and direct current (I injected in the electrodes A and B, was used for measure the potential difference between the electrodes M and N; after was calculate the resistivity point to point for obtain a subsoil tomography geoelectrical. The equipment was calibrated with minimum error (rms < 2% whit respect to curves obtained in similar commercial equipment. On this situation, in this work was modernized and automated an equipment to determine thesalinity of the subsoil. The instrument was tasted in the micro basin Texcoco, State of Mexico, to define the environment geometry formed by alluvial or lake sediments from igneous rocks (andesites, rhyolites and tuffs vitreous or “tepetates” which by its mineralogical composition allowed to the lateral resistivity be associate with free components trace from the aquifer: Cd, Cu, Cr, Co, Ni, Pb or Zn and others. This method constitutes

  6. Equipment Design of Camellia Fruit and Shell Separating by Micro-wave

    Directory of Open Access Journals (Sweden)

    Jian Zhou


    Full Text Available For obtaining excellent properties of separating Camellia fruit and shell, the micro-wave technology is used, the separating equipment is also designed. The set includes lifting raw material system, micro-wave controlling system, eliminating wet system, micro-wave affection body, dropping material system. The camellia fruits are transported into micro-wave system, then they are crashed with centrifugal throwing disc, by the affect of wind and centrifugal force, thus shells and seeds are separated obviously.

  7. ABS test equipment design%ABS 测试设备设计

    Institute of Scientific and Technical Information of China (English)



      This article simply introduces the structure and operating principle of ABS. and a new equipment for ABS signal testing is designed and put into use.%  简要介绍了 ABS 的组成及其工作原理,并结合自身情况,设计了测试 ABS 信号的设备,并投入使用。

  8. Research and design of the main equipments and structure of Xiangjiaba shiplift

    Institute of Scientific and Technical Information of China (English)

    Liao Lekang; Zhu Hong; Zhang Rui


    The type of pinion and rack vertical shiplifts has been developed in recent a couple of years in the construction of dams. But the design methods and methodologies have rarely been discussed in literature. The Xiangjiaba shiplift is the second shiplift of this type following the Three Gorges shiplift. Being aimed at the technological rationality of the design in synthetically considering security,economy and applicability,this pa-per presents the research results of some vital issues relating the design of the Xiangjiaba shiplift,including the determination of design water depth of ship chamber based on fluid numeral computation and physical model test,the optimum design of general layout of main equipments and the civil structure of the Xiangjiaba shiplift, the finite element method (FEM) analysis of stress,vibration modes and the buckling of ship chamber,anti-seismic research and the design of structures and mechanisms of the shiplift and the optimum design of driving mechanisms. This research provides the theoretical basis for the design of the Xiangjiaba shiplift. The design principles and research methods are valuable for the design of the same type of shiplifts.

  9. Equipment Design and Cost Estimation for Small Modular Biomass Systems, Synthesis Gas Cleanup, and Oxygen Separation Equipment; Task 9: Mixed Alcohols From Syngas -- State of Technology

    Energy Technology Data Exchange (ETDEWEB)

    Nexant Inc.


    This deliverable is for Task 9, Mixed Alcohols from Syngas: State of Technology, as part of National Renewable Energy Laboratory (NREL) Award ACO-5-44027, ''Equipment Design and Cost Estimation for Small Modular Biomass Systems, Synthesis Gas Cleanup and Oxygen Separation Equipment''. Task 9 supplements the work previously done by NREL in the mixed alcohols section of the 2003 technical report Preliminary Screening--Technical and Economic Assessment of Synthesis Gas to Fuels and Chemicals with Emphasis on the Potential for Biomass-Derived Syngas.

  10. Design of efficient and simple interface testing equipment for opto-electric tracking system (United States)

    Liu, Qiong; Deng, Chao; Tian, Jing; Mao, Yao


    Interface testing for opto-electric tracking system is one important work to assure system running performance, aiming to verify the design result of every electronic interface matching the communication protocols or not, by different levels. Opto-electric tracking system nowadays is more complicated, composed of many functional units. Usually, interface testing is executed between units manufactured completely, highly depending on unit design and manufacture progress as well as relative people. As a result, it always takes days or weeks, inefficiently. To solve the problem, this paper promotes an efficient and simple interface testing equipment for opto-electric tracking system, consisting of optional interface circuit card, processor and test program. The hardware cards provide matched hardware interface(s), easily offered from hardware engineer. Automatic code generation technique is imported, providing adaption to new communication protocols. Automatic acquiring items, automatic constructing code architecture and automatic encoding are used to form a new program quickly with adaption. After simple steps, a standard customized new interface testing equipment with matching test program and interface(s) is ready for a waiting-test system in minutes. The efficient and simple interface testing equipment for opto-electric tracking system has worked for many opto-electric tracking system to test entire or part interfaces, reducing test time from days to hours, greatly improving test efficiency, with high software quality and stability, without manual coding. Used as a common tool, the efficient and simple interface testing equipment for opto-electric tracking system promoted by this paper has changed traditional interface testing method and created much higher efficiency.

  11. Aquatic Equipment Information. (United States)

    Sova, Ruth

    Equipment usually used in water exercise programs is designed for variety, intensity, and program necessity. This guide discusses aquatic equipment under the following headings: (1) equipment design; (2) equipment principles; (3) precautions and contraindications; (4) population contraindications; and (5) choosing equipment. Equipment is used…

  12. A miniature MRE isolator for lateral vibration suppression of bridge monitoring equipment: design and verification (United States)

    Zhao, Lujie; Yu, Miao; Fu, Jie; Zhu, Mi; Li, Binshang


    The testing accuracy and service life of long-span bridge monitoring equipment declines over time due to the adverse effects of environmental vibration during its operation. Therefore, it is essential to use effective methods to reduce the vibration of these devices. In this paper, inspired by the controllable and field-dependent properties of magnetorheological elastomer (MRE), a miniature laminated MRE isolator is designed and manufactured to provide a relatively stable working environment for the monitoring equipment. The method and process of its specific design are elaborated in detail based on the shape factor, allowable seismic displacement, lateral stiffness, allowable vertical load and analysis of magnetic circuit. Besides, a series of dynamic tests are conducted to obtain the characteristics of the MRE isolator under various loading conditions. The experimental results show that the maximum increase of the effective stiffness is 114.12% with the current increasing from 0 A to 3 A. Consequently, the validity of its design is confirmed by a fuzzy control experiment.

  13. Re-evaluation of seismic design for JOYO buildings and equipments

    Energy Technology Data Exchange (ETDEWEB)

    Isozaki, K.; Tomita, N. [Power Reactor and Nuclear Fuel Development Corp., Oarai, Ibaraki (Japan). Oarai Engineering Center


    Hyougo-ken southern earthquake broke out in 1997/01/17. The Atomic Energy Safety Commission considered reasonable of the design guide for seismic design. And the Science and Technology Agency (STA) required reevaluation of atomic power facilities built by old design guide according to the new seismic design guide. JOYO obtained the construction license in 1970/02. Heat transport system and buildings of JOYO was re-evaluated by the new seismic design guide for the MK-III project. So, JOYO was not required re-evaluation by STA. But, this evaluation of MK-III was limited to reconstruction area, and the seismic design was reevaluated extensively to confirm earthquake proof characteristics. The structural integrity of buildings and equipments was confirmed by the result of reevaluation by the new seismic design guide. The analysis model conditions were established according to the 1987 and 1991 version of JEAG. This was done by ground investigation result and buildings vibration test. It was made clear that the analysis model conditions were reasonable and conservative from a technical view point. (author)

  14. Analysis of flow field characteristics in IC equipment chamber based on orthogonal design (United States)

    Liu, W. F.; Yang, Y. Y.; Wang, C. N.


    This paper aims to study the influence of the configuration of processing chamber as a part of IC equipment on flow field characteristics. Four parameters, including chamber height, chamber diameter, inlet mass flow rate and outlet area, are arranged using orthogonally design method to study their influence on flow distribution in the processing chamber with the commercial software-Fluent. The velocity, pressure and temperature distribution above the holder were analysed respectively. The velocity difference value of the gas flow above the holder is defined as the evaluation criteria to evaluate the uniformity of the gas flow. The quantitative relationship between key parameters and the uniformity of gas flow was found through analysis of experimental results. According to our study, the chamber height is the most significant factor, and then follows the outlet area, chamber diameter and inlet mass flow rate. This research can provide insights into the study and design of configuration of etcher, plasma enhanced chemical vapor deposition (PECVD) equipment, and other systems with similar configuration and processing condition.

  15. An Expert System for Supporting the Design and Selection of Mechanical Equipment for Recreational Crafts

    Directory of Open Access Journals (Sweden)

    T. Gonciarz


    Full Text Available Expert Systems can be defined as computer programs, whose main task is to simulate a human expert, usually in a narrow field of expertise. Expert Systems have experienced tremendous growth and popularity since their commercial introduction in the early 1970’s. Today, Expert Systems are used in business, science, engineering, manufacturing and other engineering applications such as planning, scheduling, diagnosing equipment failures and are used in almost every stage of the manufacturing process and also in medicine and many other fields. Expert Systems belong to the field of artificial intelligence. An intelligent computer program that uses knowledge and inference procedures to solve problems that are difficult enough to require significant human expertise for the solution. The purpose of this paper is to present an Expert System which assists with the design of yachts and supports the selection of mechanical equipment for yachts and includes knowledge in the field of yachting engineering. Using the presented Expert System reduces the time during the design and production preparation process.

  16. Stereoselective assembly of amino acid-based metal-biomolecule nanofibers. (United States)

    Wu, Hong; Tian, Chunyong; Zhang, Yufei; Yang, Chen; Zhang, Songping; Jiang, Zhongyi


    A series of amino acid-based metal-biomolecule nanofibers are fabricated through a coordination-directed assembly process. The chirality and carbon chain length of the amino acids exert a pronounced influence on the assembly process. This study may be extended to design diverse kinds of 1-D metal-biomolecule frameworks (MBioFs).

  17. Supramolecular Assemblies Responsive to Biomolecules toward Biological Applications. (United States)

    Shigemitsu, Hajime; Hamachi, Itaru


    Stimuli-responsive supramolecular assemblies consisting of small molecules are attractive functional materials for biological applications such as drug delivery, medical diagnosis, enzyme immobilization, and tissue engineering. By use of their dynamic and reversible properties, many supramolecular assemblies responsive to a variety of biomolecules have been designed and synthesized. This review focuses on promising strategies for the construction of such dynamic supramolecular assemblies and their functions. While studies of biomolecule-responsive supramolecular assemblies have mainly been performed in vitro, it has recently been demonstrated that some of them can work in live cells. Supramolecular assemblies now open up new avenues in chemical biology and biofunctional materials.

  18. The Design and Implementation of the Remote Centralized-Monitoring System of Well-Control Equipment Based on RFID Technique

    Directory of Open Access Journals (Sweden)

    Luo Bin


    Full Text Available At present, in domestic for the management of well control equipment continue to the traditional way of nameplates identifies and paper-based registration, there are many issues like the separation of data information of device, easy lose, difficult query, confused management and many other problems, which will make the problem device into the well field, and then resulting in well control runaway drilling accident. To solve the above problems, this paper put forward to the integrated remote centralized-monitoring management mode of the well-control equipment. Taking the advantages of IOT technology, adopting the RFID technology, and combining with the remote transmission, this paper designs the remote centralized-monitoring system of well-control equipment based on RFID, which realizes the intelligent management of well-control equipment and meets the actual demand of the well-control equipment safe use and timely scheduling, and it has the ability of field application.

  19. Design and Experiment on Self-propelled Precise Feeding Equipment for Dairy Cow

    Directory of Open Access Journals (Sweden)

    Hewei Meng


    Full Text Available Designed a kind of self-propelled precise feeding machine for single dairy cow based on the technology of RFID, to achieve the automation,fine and intelligent of dairy farming.The computer was used as the information management platform, MCU was used as control platform, even using wireless transmission, RFID recognition, infrared detection technology and so on, which achievement the information data of wireless transmission,precise recognition and detection cattle position.It is applied to equal-diameter and variable-pitch screw feeding structure to realize the precise concentrated feed supply, equipment performance test shown that the system speed 60rpm is the most stable when feeding, feeding accuracy not less than 97.5%, to meet the feeding requirements, equipped with the best traveling speed is 0.6m/s, the response time of the system is 0.4s, the recognition rate is 96%; through one-month feeding experiment in the dairy cow farm showed that the milk production was increased, the average daily milk yield of individual cows improve 0.8kg than artificial feeding.

  20. Fundamentals of Biomolecule Analysis by Electrospray Ionization Mass Spectrometry (United States)

    Weinecke, Andrea; Ryzhov, Victor


    Electrospray ionization (ESI) is a soft ionization technique that allows transfer of fragile biomolecules directly from solution into the gas phase. An instrumental analysis laboratory experiment is designed that would introduce the students to the ESI technique, major parameters of the ion trap mass spectrometers and some caveats in…

  1. Thermally modulated biomolecule transport through nanoconfined channels. (United States)

    Liu, Lei; Zhu, Lizhong


    In this work, a nanofluidic device containing both a feed cell and a permeation cell linked by nanopore arrays has been fabricated, which is employed to investigate thermally controlled biomolecular transporting properties through confined nanochannels. The ionic currents modulated by the translocations of goat antibody to human immunoglobulin G (IgG) or bovine serum albumin (BSA) are recorded and analyzed. The results suggest that the modulation effect decreases with the electrolyte concentration increasing, while the effects generated by IgG translocation are more significant than that generated by BSA translocation. More importantly, there is a maximum decreasing value in each modulated current curve with biomolecule concentration increasing for thermally induced intermolecular collision. Furthermore, the turning point for the maximum shifts to lower biomolecule concentrations with the system temperature rising (from 4°C to 45°C), and it is mainly determined by the temperature in the feed cell if the temperature difference exists in the two separated cells. These findings are expected to be valuable for the future design of novel sensing device based on nanopore and/or nanopore arrays.

  2. Max Tech and Beyond: Maximizing Appliance and Equipment Efficiency by Design

    Energy Technology Data Exchange (ETDEWEB)

    Desroches, Louis-Benoit; Garbesi, Karina


    It is well established that energy efficiency is most often the lowest cost approach to reducing national energy use and minimizing carbon emissions. National investments in energy efficiency to date have been highly cost-effective. The cumulative impacts (out to 2050) of residential energy efficiency standards are expected to have a benefit-to-cost ratio of 2.71:1. This project examined energy end-uses in the residential, commercial, and in some cases the industrial sectors. The scope is limited to appliances and equipment, and does not include building materials, building envelopes, and system designs. This scope is consistent with the scope of DOE's appliance standards program, although many products considered here are not currently subject to energy efficiency standards. How much energy could the United States save if the most efficient design options currently feasible were adopted universally? What design features could produce those savings? How would the savings from various technologies compare? With an eye toward identifying promising candidates and strategies for potential energy efficiency standards, the Max Tech and Beyond project aims to answer these questions. The analysis attempts to consolidate, in one document, the energy savings potential and design characteristics of best-on-market products, best-engineered products (i.e., hypothetical products produced using best-on-market components and technologies), and emerging technologies in research & development. As defined here, emerging technologies are fundamentally new and are as yet unproven in the market, although laboratory studies and/or emerging niche applications offer persuasive evidence of major energy-savings potential. The term 'max tech' is used to describe both best-engineered and emerging technologies (whichever appears to offer larger savings). Few best-on-market products currently qualify as max tech, since few apply all available best practices and components. The

  3. HVAC Equipment Design Options for Near-Zero-Energy Homes (NZEH) -A Stage 2 Scoping Assessment

    Energy Technology Data Exchange (ETDEWEB)

    Baxter, Van D [ORNL


    Although the energy efficiency of heating, ventilating, and air-conditioning (HVAC) equipment has increased substantially in recent years, new approaches are needed to continue this trend. Conventional unitary equipment and system designs have matured to a point where cost-effective, dramatic efficiency improvements that meet near-zero-energy housing (NZEH) goals require a radical rethinking of opportunities to improve system performance. The large reductions in HVAC energy consumption necessary to support the NZEH goals require a systems-oriented analysis approach that characterizes each element of energy consumption, identifies alternatives, and determines the most cost-effective combination of options. In particular, HVAC equipment must be developed that addresses the range of special needs of NZEH applications in the areas of reduced HVAC and water heating energy use, humidity control, ventilation, uniform comfort, and ease of zoning. This report describes results of a scoping assessment of HVAC system options for NZEH homes. ORNL has completed a preliminary adaptation, for consideration by The U.S. Department of Energy, Energy Efficiency and Renewable Energy Office, Building Technologies (BT) Program, of Cooper's (2001) stage and gate planning process to the HVAC and Water Heating element of BT's multi-year plan, as illustrated in Figure 1. In order to adapt to R&D the Cooper process, which is focused on product development, and to keep the technology development process consistent with an appropriate role for the federal government, the number and content of the stages and gates needed to be modified. The potential federal role in technology development involves 6 stages and 7 gates, but depending on the nature and status of the concept, some or all of the responsibilities can flow to the private sector for product development beginning as early as Gate 3. In the proposed new technology development stage and gate sequence, the Stage 2 &apos

  4. Equipment Design and Cost Estimation for Small Modular Biomass Systems, Synthesis Gas Cleanup, and Oxygen Separation Equipment; Task 1: Cost Estimates of Small Modular Systems

    Energy Technology Data Exchange (ETDEWEB)

    Nexant Inc.


    This deliverable is the Final Report for Task 1, Cost Estimates of Small Modular Systems, as part of NREL Award ACO-5-44027, ''Equipment Design and Cost Estimation for Small Modular Biomass Systems, Synthesis Gas Cleanup and Oxygen Separation Equipment''. Subtask 1.1 looked into processes and technologies that have been commercially built at both large and small scales, with three technologies, Fluidized Catalytic Cracking (FCC) of refinery gas oil, Steam Methane Reforming (SMR) of Natural Gas, and Natural Gas Liquids (NGL) Expanders, chosen for further investigation. These technologies were chosen due to their applicability relative to other technologies being considered by NREL for future commercial applications, such as indirect gasification and fluidized bed tar cracking. Research in this subject is driven by an interest in the impact that scaling has on the cost and major process unit designs for commercial technologies. Conclusions from the evaluations performed could be applied to other technologies being considered for modular or skid-mounted applications.

  5. Design Frame of Equipment Operation Test Concept%装备作战试验概念设计框架

    Institute of Scientific and Technical Information of China (English)

    罗小明; 池建军; 周跃


    提出了装备作战试验目标、内容、流程和方法、组织指挥、评估的5维概念设计框架。从提高装备综合战技性能、确定装备编配运用原则、检验优化装备体系结构3个方面,明确了装备作战试验的目标定位;对装备作战适用性试验和装备作战效能试验2项主要内容进行了论释;提出了装备作战试验的组织实施流程,列出了外场实装、内场仿真、等效推算和一体化试验方法;构建了基于信息系统的装备作战试验组织指挥能力的构成框架;给出了装备作战试验评估的基本思路和主要步骤。可为装备作战试验顶层设计和创新研究提供新的手段。%The paper presents a 5-demensions concept design frame of equipment operation test, which ineludes the target, content, process & method, organization & command, and evaluation. It analyses the goal orientation of equipment operation test from three aspects of improving equipment synthetical tactics and technique performance, fixing on equipment arranging and managing principle, checking up and optimizing equipment system configuration. It explains two main contents of equipment operation applicability test and equipment operation efficiency test, advances the organization and execution process of equipment operation test, lists out the basic methods which include outfield installation method, infield simulation method, equivalent calculation method, integrative method. The equipment operation test organization and command capability structure frame is built based on information system, the equipment operation test evaluation basic thought and main steps are concluded, which provides new measure for equipment operation test top design and innovation research.

  6. Design challenges for matrix assisted pulsed laser evaporation and infrared resonant laser evaporation equipment (United States)

    Greer, James A.


    Since the development of the Matrix Assisted Pulsed Laser Evaporation (MAPLE) process by the Naval Research Laboratory (NRL) in the late 1990s, MAPLE has become an active area of research for the deposition of a variety of polymer, biological, and organic thin films. As is often the case with advancements in thin-film deposition techniques new technology sometimes evolves by making minor or major adjustments to existing deposition process equipment and techniques. This is usually the quickest and least expensive way to try out new ideas and to "push the envelope" in order to obtain new and unique scientific results as quickly as possible. This process of "tweaking" current equipment usually works to some degree, but once the new process is further refined overall designs for a new deposition tool based on the critical attributes of the new process typically help capitalize more fully on the all the salient features of the new and improved process. This certainly has been true for the MAPLE process. In fact the first MAPLE experiments the polymer/solvent matrix was mixed and poured into a copper holder held at LN2 temperature on a laboratory counter top. The holder was then quickly placed onto a LN2 cooled reservoir in a vacuum deposition chamber and placed in a vertical position on a LN2 cooled stage and pumped down as quickly as possible. If the sample was not placed into the chamber quickly enough the frozen matrix would melt and drip into the bottom of the chamber onto the chambers main gate valve making a bit of a mess. However, skilled and motivated scientists usually worked quickly enough to make this process work most of the time. The initial results from these experiments were encouraging and led to several publications which sparked considerable interest in this newly developed technique Clearly this approach provided the vision that MAPLE was a viable deposition process, but the equipment was not optimal for conducting MAPLE experiments on a regular basis

  7. Design guidelines for use of adhesives in hybrid microcircuits. [for electronic equipment in space applications (United States)

    Caruso, S. V.; Perkins, K. L.; Licari, J. J.


    Although it is generally accepted that the use of adhesives in the assembly of hybrid microcircuits offers advantages over other bonding methods, there currently does not exist a set of guidelines for the selection of adhesives which will insure that hybrid microcircuits assembled with them will meet the long use-life, high-reliability requirements of electronic equipment for space applications. This study was directed to the identification of the properties of electrically insulative adhesives that potentially could cause problems in such an application, and to the development of evaluation tests to quantify these properties and thus form the basis for establishing suitable guidelines and, ultimately, specifications. Bond strength, outgassing after cure, and corrosivity were selected for detailed attention since they are considered to be especially critical. Introductory discussion includes enumeration and brief comments on the properties of adhesives considered to be important for the proposed application, a general review of polymeric types of adhesives, and identification of the major types of adhesives commercially available and specifically designed for microelectronic use. The specific tests developed to evaluate bond strength, outgassing after cure, and corrosivity are discussed in detail, and comparative results obtained for selected adhesives representative of the major types are given.

  8. 30 CFR 75.1909 - Nonpermissible diesel-powered equipment; design and performance requirements. (United States)


    ... ADMINISTRATION, DEPARTMENT OF LABOR COAL MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES... rail-mounted equipment, must be provided with a parking brake that holds the fully loaded equipment... work platforms must be provided with a means to ensure that the parking braking system is...

  9. Optical aptasensors for quantitative detection of small biomolecules: a review. (United States)

    Feng, Chunjing; Dai, Shuang; Wang, Lei


    Aptasensors are aptamer-based biosensors with excellent recognition capability towards a wide range of targets. Specially, there have been ever-growing interests in the development of aptasensors for the detection of small molecules. This phenomenon is contributed to two reasons. On one hand, small biomolecules play an important role in living organisms with many kinds of biological function, such as antiarrhythmic effect and vasodilator activity of adenosine. On the other hand, the concentration of small molecules can be an indicator for disease diagnosis, for example, the concentration of ATP is closely associated with cell injury and cell viability. As a potential analysis tool in the construction of aptasensors, optical analysis has attracted much more interest of researchers due to its high sensitivity, quick response and simple operation. Besides, it promises the promotion of aptasensors in performance toward a new level. Review the development of optical aptasensors for small biomolecules will give readers an overall understanding of its progress and provide some theoretical guidelines for its future development. Hence, we give a mini-review on the advance of optical aptasensors for small biomolecules. This review focuses on recent achievements in the design of various optical aptasensors for small biomolecules, containing fluorescence aptasensors, colorimetric aptasensors, chemiluminescence aptasensors and other optical aptasensors.

  10. Design, characterization and applications of new ionic liquid matrices for multifunctional analysis of biomolecules: A novel strategy for pathogenic bacteria biosensing

    Energy Technology Data Exchange (ETDEWEB)

    Abdelhamid, Hani Nasser [Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan (China); Department of Chemistry, Assuit University, Assuit, 71515 (Egypt); Khan, M Shahnawaz [Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan (China); Wu, Hui-Fen, E-mail: [Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan (China); School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 800, Taiwan (China); Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan (China); Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan (China); Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan (China)


    Highlights: • Design and characterize novel UV absorbed-ionic liquid matrices series. • Apply the new series for different analytes. • Introduce a novel methodology for pathogenic bacteria biosensing. • Tabulate the physical parameters of the new series. - Abstract: The design, preparation and performance for novel UV-light absorbing (room-temperature) ionic liquid matrices (UV-RTILMs) for matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) were reported. A series of UV-RTILMs was prepared by ultrasonication of equimolar of acid (mefenamic acid) and bases (aniline (ANI), pyridine (Pyr), dimethyl aniline (DMANI) and 2-methyl picoline (2-P)). The UV-RTILMs have not only significant absorbance at the desired wavelength (337 nm of the N{sub 2} Laser), but also have available protons that can easily undergo proton transfer reactions to ionize the target molecules. The novel UV-RTILMs have the ability to ionize different and wide classes of compounds such as drugs, carbohydrate, and amino acids. The new UV-RTILMs series have been successfully and selectively applied for biosensing the lysates of pathogenic bacteria in the presence of the cell macromolecules. A new strategy for biosensing pathogens was presented via sensing the pathogens lysate in the cell suspension. The new materials can effectively detect the bacterial toxins without separation or any pretreatment. They offered excellent ionization of labile oligosaccharides with protonated peaks. They could significantly enhance the analyte signals, produce homogeneous spotting, reducing spot-to-spot variation, excellent vacuum stability, higher ion peak intensity, and wide application possibility. The physical parameters such as molar refractivity, molar volume, parachor, surface tension, density and polarizability were calculated and tabulated. The new UV-RTILMs could offer excellent reproducibility and great repeatability and they are promising matrices for wide applications on MALDI-MS.

  11. Key Issues in Building Design: How to Get Started in Planning a Project. Library Buildings and Equipment Section (United States)

    Dahlgren, Anders C.; Eigenbrodt, Olaf; Latimer, Karen; Romero, Santi


    Based on the IFLA (International Federation of Library Associations and Institutions) Library Buildings and Equipment Section's Library Building Guidelines published in 2007, this short publication summarises the key points to take into consideration when designing a new or refurbished library building. The aim of the brochure is not to replace…

  12. Biomolecule-based nanomaterials and nanostructures. (United States)

    Willner, Itamar; Willner, Bilha


    Biomolecule-nanoparticle (or carbon nanotube) hybrid systems provide new materials that combine the unique optical, electronic, or catalytic properties of the nanoelements with the recognition or biocatalytic functions of biomolecules. This article summarizes recent applications of biomolecule-nanoparticle (or carbon nanotubes) hybrid systems for sensing, synthesis of nanostructures, and for the fabrication of nanoscale devices. The use of metallic nanoparticles for the electrical contacting of redox enzymes with electrodes, and as catalytic labels for the development of electrochemical biosensors is discussed. Similarly, biomolecule-quantum dot hybrid systems are implemented for optical biosensing, and for monitoring intracellular metabolic processes. Also, the self-assembly of biomolecule-metal nanoparticle hybrids into nanostructures and functional nanodevices is presented. The future perspectives of the field are addressed by discussing future challenges and highlighting different potential applications.

  13. Selective chromogenic detection of thiol-containing biomolecules using carbonaceous nanospheres loaded with silver nanoparticles as carrier. (United States)

    Hu, Bo; Zhao, Yang; Zhu, Hai-Zhou; Yu, Shu-Hong


    Thiol-containing biomolecules show strong affinity with noble metal nanostructures and could not only stably protect them but also control the self-assembly process of these special nanostructures. A highly selective and sensitive chromogenic detection method has been designed for the low and high molecular weight thiol-containing biomolecules, including cysteine, glutathione, dithiothreitol, and bovine serum albumin, using a new type of carbonaceous nanospheres loaded with silver nanoparticles (Ag NPs) as carrier. This strategy relies upon the place-exchange process between the reporter dyes on the surface of Ag NPs and the thiol groups of thiol-containing biomolecules. The concentration of biomolecules can be determined by monitoring with the fluorescence intensity of reporter dyes dispersed in solution. This new chromogenic assay method could selectively detect these biomolecules in the presence of various other amino acids and monosaccharides and even sensitively detect the thiol-containing biomolecules with different molecular weight, even including proteins.

  14. Research on Modularized Design and Allocation of Infectious Disease Prevention and Control Equipment in China. (United States)

    Zhao, Xin; Wang, Yun-Dou; Zhang, Xiao-Feng; Gao, Shu-Tian; Guo, Li-Jun; Sun, Li-Na


    For the prevention and control of newly emergent or sudden infectious diseases, we built an on-site, modularized prevention and control system and tested the equipment by using the clustering analysis method. On the basis of this system, we propose a modular equipment allocation method and 4 applications of this method for different types of infectious disease prevention and control. This will help to improve the efficiency and productivity of anti-epidemic emergency forces and will provide strong technical support for implementing more universal and serialized equipment in China. (Disaster Med Public Health Preparedness. 2016; page 1 of 8).

  15. Effect of extraction solvents on the biomolecules and antioxidant properties of Scorzonera undulata (Asteraceae: Application of factorial design optimization phenolic extraction

    Directory of Open Access Journals (Sweden)

    Khaled Athmouni


    Full Text Available Background. Phenolic compounds were extracted and isolated from S. undulata roots. Methods. Sample of roots from E. hirta was tested for phenolic compounds, and in vitro antioxidant activity by diphenyl-1-picrylhydrazyl (DPPH assay, ABTS, FRAP and reducing power was measured using cyano- ferrate method. Results. The methanolic fraction exhibited the highest total phenol content (6.12 ±0.11 mg AGE/g DW. On the other hand, the highest flavonoids concentration was observed in ethyl acetate fraction (2.90 ±0.05 mg CE/g DW in addition to anthocyanins (28.56 ±3.96 mg/l. Besides, the highest level of tannins content was measured in the polar aprotic solvent ethyl acetate extract (3.25 ±0.06 mg CE/g DW. The different extracts of S. undulata were evaluated for their radical scavenging activities by means of the DPPH assay. The strongest scavenging activity was observed in methanolic fraction scavenged radicals effectively with IC   values of 0.14 ±0.02 mg/ml. Similarly, the potassium ferricyanide reduction (FRAP and ABTS•+ of methanol extract. On the other hand, the total reducing power of ethyl acetate extract was found higher than of other extracts. This paper presents the application of the design-of experiment method for optimizing the extraction of phe- nolic content using methanol solvent. The resulting regression model has shown that the effect of temperature is not statistically significant (with >95% certainty, while that of agitation speed is. The two main effects are contributed by the solvent concentration and the maceration period. Conclusion. Our results clearly showed that the extraction of phenolic compounds and their antioxidant ca- pacity is significantly affected by solvent combinations. S. undulata presented the highest total phenolic con- tent, total flavonoids content and antioxidant capacity values. The resulting regression model has shown that the effect of temperature is not statistically significant (with >95

  16. Improved design of electrophoretic equipment for rapid sickle-cell-anemia screening (United States)

    Reddick, J. M.; Hirsch, I.


    Effective mass screening may be accomplished by modifying existing electrophoretic equipment in conjunction with multisample applicator used with cellulose-acetate-matrix test paper. Using this method, approximately 20 to 25 samples can undergo electrophoresis in 5 to 6 minutes.

  17. Using Some Coupled Numerical Models in Problems of Designing an Inductive Electrothermal Equipment

    Directory of Open Access Journals (Sweden)

    LEUCA Teodor


    Full Text Available This paper focuses on the numerical modeling of coupling the electromagnetic and the thermal field, in the process of inductive heating, for inductive electrothermal equipments. Numerical results are carried out by using a FLUX2D application.

  18. Design of online monitoring and forecasting system for electrical equipment temperature of prefabricated substation based on WSN (United States)

    Qi, Weiran; Miao, Hongxia; Miao, Xuejiao; Xiao, Xuanxuan; Yan, Kuo


    In order to ensure the safe and stable operation of the prefabricated substations, temperature sensing subsystem, temperature remote monitoring and management subsystem, forecast subsystem are designed in the paper. Wireless temperature sensing subsystem which consists of temperature sensor and MCU sends the electrical equipment temperature to the remote monitoring center by wireless sensor network. Remote monitoring center can realize the remote monitoring and prediction by monitoring and management subsystem and forecast subsystem. Real-time monitoring of power equipment temperature, history inquiry database, user management, password settings, etc., were achieved by monitoring and management subsystem. In temperature forecast subsystem, firstly, the chaos of the temperature data was verified and phase space is reconstructed. Then Support Vector Machine - Particle Swarm Optimization (SVM-PSO) was used to predict the temperature of the power equipment in prefabricated substations. The simulation results found that compared with the traditional methods SVM-PSO has higher prediction accuracy.

  19. Development of time-resolved laser-induced fluorescence spectroscopic technique for the analysis of biomolecules

    Directory of Open Access Journals (Sweden)

    Terzić M.


    Full Text Available Our developments of the time-resolved laser-induced fluorescence (TR-LIF detection system for biomolecules are presented. This system is based on the tunable (320 nm to 475 nm Nd:YAG laser pulses used to excite various biomolecules. The detection part is the Streak System for Fluorescence Lifetime Spectroscopy (Hamamatsu, Japan. The system consists of a C4334-01 streakscope, as a detector, DG 535 digital pulse/delay generator, C5094-S Spectrograph and HPD-TA System, as a temporal analyzer. The TR-LIF spectrometer is designed primarily to study the temperature and pressure effects on fluorescence behavior of biomolecules upon excitation with a single nanosecond pulse. The design of this system has capability to combine laser-induced breakdown (LIB with fluorescence, as well to study optodynamic behavior of fluorescence biomolecules.

  20. Biomolecule-recognition gating membrane using biomolecular cross-linking and polymer phase transition. (United States)

    Kuroki, Hidenori; Ito, Taichi; Ohashi, Hidenori; Tamaki, Takanori; Yamaguchi, Takeo


    We present for the first time a biomolecule-recognition gating system that responds to small signals of biomolecules by the cooperation of biorecognition cross-linking and polymer phase transition in nanosized pores. The biomolecule-recognition gating membrane immobilizes the stimuli-responsive polymer, including the biomolecule-recognition receptor, onto the pore surface of a porous membrane. The pore state (open/closed) of this gating membrane depends on the formation of specific biorecognition cross-linking in the pores: a specific biomolecule having multibinding sites can be recognized by several receptors and acts as the cross-linker of the grafted polymer, whereas a nonspecific molecule cannot. The pore state can be distinguished by a volume phase transition of the grafted polymer. In the present study, the principle of the proposed system is demonstrated using poly(N-isopropylacrylamide) as the stimuli-responsive polymer and avidin-biotin as a multibindable biomolecule-specific receptor. As a result of the selective response to the specific biomolecule, a clear permeability change of an order of magnitude was achieved. The principle is versatile and can be applied to many combinations of multibindable analyte-specific receptors, including antibody-antigen and lectin-sugar analogues. The new gating system can find wide application in the bioanalytical field and aid the design of novel biodevices.

  1. Recent advances in exploiting ionic liquids for biomolecules: Solubility, stability and applications. (United States)

    Sivapragasam, Magaret; Moniruzzaman, Muhammad; Goto, Masahiro


    The technological utility of biomolecules (e.g. proteins, enzymes and DNA) can be significantly enhanced by combining them with ionic liquids (ILs) - potentially attractive "green" and "designer" solvents - rather than using in conventional organic solvents or water. In recent years, ILs have been used as solvents, cosolvents, and reagents for biocatalysis, biotransformation, protein preservation and stabilization, DNA solubilization and stabilization, and other biomolecule-based applications. Using ILs can dramatically enhance the structural and chemical stability of proteins, DNA, and enzymes. This article reviews the recent technological developments of ILs in protein-, enzyme-, and DNA-based applications. We discuss the different routes to increase biomolecule stability and activity in ILs, and the design of biomolecule-friendly ILs that can dissolve biomolecules with minimum alteration to their structure. This information will be helpful to design IL-based processes in biotechnology and the biological sciences that can serve as novel and selective processes for enzymatic reactions, protein and DNA stability, and other biomolecule-based applications.

  2. A Knowledge Based System for the Design of Mobile Subscriber Equipment Communications Networks. (United States)


    organic chemistry , and medical diagnosis (20:5-6). Expert systems are generally composed of three parts: a database containing the specialized domain...Pennsylvania, 26 March 1984. 31. Schaum , Don and others. "MSE: Mobile Subscriber Equipment," Army Communicator, 9: 6-22 (Fall 1984). 32. Schneider, G

  3. Immobilizing Biomolecules Near the Diffraction Limit

    DEFF Research Database (Denmark)

    Skovsen, Esben; Petersen, Maria Teresa Neves; Gennaro, Ane Kold Di


    Our group has previously shown that biomolecules containing disulfide bridges in close proximity to aromatic residues can be immobilized, through covalent bonds, onto thiol derivatized surfaces upon UV excitation of the aromatic residue(s). We have also previously shown that our new technology can...... be used to print arrays of biomolecules and to immobilize biomolecules according to any specific pattern on a planar substrates with micrometer scale resolution. In this paper we show that we can immobilize proteins according to diffraction patterns of UV light. We also show that the feature size...... of the immobilized patterns can be as small as the diffraction limit for the excitation light, and that the immobilized patterns correspond to the diffraction pattern used to generate it. The flexibility of this new technology will in principle make it possible to create any pattern of biomolecules onto a substrate...

  4. An overview: biomolecules from microalgae for animal feed and aquaculture. (United States)

    Yaakob, Zahira; Ali, Ehsan; Zainal, Afifi; Mohamad, Masita; Takriff, Mohd Sobri


    Despite being more popular for biofuel, microalgae have gained a lot of attention as a source of biomolecules and biomass for feed purposes. Algae farming can be established using land as well as sea and strategies can be designed in order to gain the products of specific interest in the optimal way. A general overview of the contributions of Algae to meet the requirements of nutrients in animal/aquaculture feed is presented in this study. In addition to its applications in animal/aquaculture feed, algae can produce a number of biomolecules including astaxanthin, lutein, beta-carotene, chlorophyll, phycobiliprotein, Polyunsaturated Fatty Acids (PUFAs), beta-1,3-glucan, and pharmaceutical and nutraceutical compounds which have been reviewed with respect to their commercial importance and current status. The review is further extended to highlight the adequate utilization of value added products in the feeds for livestock, poultry and aquaculture (with emphasis in shrimp farming).

  5. Impact of Mars sand on dust on the design of space suits and life support equipment: A technology assessment (United States)

    Simonds, Charles H.


    Space suits and life support equipment will come in intimate contact with Martian soil as aerosols, wind blown particles and material thrown up by men and equipment on the Martian surface. For purposes of this discussion the soil is assumed to consist of a mixture of cominuted feldspar, pyroxene, olivine, quartz, titanomagnetite and other anhydrous and hydrous iron bearing oxides, clay minerals, scapolite and water soluble chlorides and sulfates. The soil may have photoactivated surfaces that acts as a strong oxidizer with behavior similar to hydrogen peroxide. The existing data about the Mars soil suggests that the dust and sand will require designs analogous to those uses on equipment exposed to salty air and blowing sand and dust. The major design challenges are in developing high performance radiators which can be cleaned after each EVA without degradation, designing seals that are readily cleaned and possibly in selecting materials which will not be degraded by any strong oxidants in the soil. The magnitude of the dust filtration challenge needs careful evaluation in terms of the trade off between fine-particle dust filters with low pressure drop that are either physically large and heavy, like filter baghouses require frequent replacement of filter elements, of low volume high pressure thus power consumption approaches, or washable filters. In the latter, filter elements are cleaned with water, as could the outsides of the space suits in the airlock.

  6. Optoelectrofluidic Manipulation of Nanoparticles and Biomolecules

    Directory of Open Access Journals (Sweden)

    Hyundoo Hwang


    Full Text Available This paper presents optoelectrofluidic technologies for manipulation of nanoparticles and biomolecules. Optoelectrofluidics provides an elegant scheme for the programmable manipulation of particles or fluids in microenvironments based on optically induced electrokinetics. Recent progress on the optoelectrofluidic manipulation of nanoobjects, which include nanospheres, nanowires, nanotubes, and biomolecules, is introduced. Some potential applications of the optoelectrofluidic nanoparticle manipulation, such as nanoparticles separation, nanostructures manufacturing, molecular physics, and clinical diagnostics, and their future directions are also discussed.

  7. Biomolecule/nanomaterial hybrid systems for nanobiotechnology. (United States)

    Tel-Vered, Ran; Yehezkeli, Omer; Willner, Itamar


    The integration of biomolecules with metallic or semiconductor nanoparticles or carbon nanotubes yields new hybrid nanostructures of unique features that combine the properties of the biomolecules and of the nano-elements. These unique features of the hybrid biomolecule/nanoparticle systems provide the basis for the rapid development of the area of nanobiotechnology. Recent advances in the implementation of hybrid materials consisting of biomolecules and metallic nanoparticles or semiconductor quantum dots will be discussed. The following topics will be exemplified: (i) The electrical wiring of redox enzymes with electrodes by means of metallic nanoparticles or carbon nanotubes, and the application of the modified electrodes as amperometric biosensors or for the construction of biofuel cells. (ii) The biocatalytic growth of metallic nanoparticles as a means to construct optical or electrical sensors. (iii) The functionalization of semiconductor quantum dots with biomolecules and the application of the hybrid nanostructures for developing different optical sensors, including intracellular sensor systems. (iv) The use of biomolecule-metallic nanoparticle nanostructures as templates for growing metallic nanowires, and the construction of fuel-driven nano-transporters.

  8. Potentiometric sensors doped with biomolecules as a new approach to small molecule/biomolecule binding kinetics analysis. (United States)

    Daems, D; De Wael, K; Vissenberg, K; Van Camp, G; Nagels, L


    The most successful binding kinetics analysis systems at this moment include surface plasmon resonance (SPR), quartz microcrystal balance (QMB) and surface acoustic wave (SAW). Although these are powerful methods, they generally are complex, expensive and require the use of monolayers. Here, we report on potentiometric sensors as an inexpensive and simple alternative to do binding kinetics analysis between small molecules in solution and biomolecules (covalently) attached in a biopolymer sensor coating layer. As an example, dopamine and an anti-dopamine aptamer were used as the small molecule and the biomolecule respectively. Binding between both follows a Langmuir adsorption type model and creates a surface potential. The system operates in Flow Injection Analysis mode (FIA). Besides being an interesting new binding kinetics tool, the approach allows systematic design of potentiometric biosensors (in the present study a dopamine sensor), and gives new insights into the functioning of ion-selective electrodes (ISE's).

  9. Interaction and educational design of mobile equipment for crisis management training

    DEFF Research Database (Denmark)

    Ørngreen, Rikke


    As researchers and designers of crisis management training in mobile learning settings, we need to consider ways in which principles of educational design and interaction design can co-exist, in order to reach a higher quality of the analysis and design process. The paper is an input to this disc......As researchers and designers of crisis management training in mobile learning settings, we need to consider ways in which principles of educational design and interaction design can co-exist, in order to reach a higher quality of the analysis and design process. The paper is an input...

  10. Solid state microwave modules designed for millimiter wave electronic equipment and systems

    Directory of Open Access Journals (Sweden)

    Karushkin N. F.


    Full Text Available The paper presents scientific, technological and production potential of Research Institute «Orion» in the field of creation of wide range of high performance active and passive solid-state microwave devices, modules and components as well as multifunctional devices on their basis. These products taken as a whole form a component base for promising equipment and systems working in the frequency range from 1.0 to 200 GHz.

  11. Design and Application of an Expert System for Equipment Maintenance and Forecast

    Institute of Scientific and Technical Information of China (English)

    WANG Jian; PAN Kai-long; SHEN Yun-feng; LI Jie


    The maintenance and forecast expert system of equipment based on Artificial Neural Network is composed of control, measure, failure forecast, execution, data processing module and database. The data processing module obtains the change of the controlled objects′ structure and parameters, then takes correspondent measures according to the examination and diagnosis information. The failure forecast module finds the control system fault, separates the fault symptom location, tells the fault kind, estimates the magnitude and time of the fault, and finally makes evaluation and decision.

  12. The Food and Drug Administration's initiative for safe design and effective use of home medical equipment. (United States)

    Weick-Brady, Mary; Singh, Simran


    Although home-use medical devices provide significant benefits, including improved quality of life and cost savings, they are associated with unique risks. These risks result from interactions among the user, the use environment, and the device, and they can greatly impact user and patient safety. This article describes measures being taken by the Food and Drug Administration to address safe use of medical equipment by trained and untrained people outside of clinical facilities.

  13. Equipment for Nonlinear Photonics Research - Light Control and Image Transmission in Specially-Designed Photonic (United States)


    J. M. Zeuner, D. Song, Z. Chen, A. Szameit and M. Segev, "Topological Creation and Destruction of Edge States in Photonic Graphene ", Phys. Rev... photonic graphene ”, Nature Material,13, 57 (2014) ( published online: 10 November 2013 | doi:10.1038/nmat3783) • X. Qi, K.G. Makris, R. El-Ganainy...AFRL-OSR-VA-TR-2014-0068 Equipment for Nonlinear Photonics Research Zhigang Chen SAN FRANCISCO STATE UNIVERSITY Final Report 03/14/2014 DISTRIBUTION

  14. Design and Experiment on Bionic Chewing Equipment%仿生咀嚼装置设计与试验

    Institute of Scientific and Technical Information of China (English)

    孙钟雷; 孙永海; 万鹏; 李君兴


    模仿人类咀嚼系统,利用仿生技术设计了一种用于粉碎食物的仿生咀嚼装置.采用逆向工程方法设计制作了仿生牙齿、仿生颞下颌关节等零部件,并组装成咀嚼装置,实现了三维咀嚼运动,确定了运动参数.咀嚼功能测试结果表明,本装置的咬合力可达到咀嚼食物的力量,并且可根据食物的不同而改变;本装置的咀嚼效率和受试者无显著差异,最大值可达92.3%;重复试验无显著差异,本装置稳定可靠.%A bionic chewing equipment was developed for food breakdown by simulating the masticatory system and using the bionic technology. Some parts were designed and assembled by reverse engineering method, like bionic teeth and temporomandibular joints. The equipment could realize 3-D chewing motion, the movement parameters were determined. Test of occlusive force showed that chewing force of equipment could break food and change with food. Masticatory experiment showed that masticatory efficiency of the equipment had no significant difference with subjects and its maximum value was 92. 3% . The bionic chewing equipment was stable and reliable.

  15. Functional nanoprobes for ultrasensitive detection of biomolecules: an update. (United States)

    Xu, Jing-Juan; Zhao, Wei-Wei; Song, Shiping; Fan, Chunhai; Chen, Hong-Yuan


    With the rapidly increasing demands for ultrasensitive biodetection, the design and applications of functional nanoprobes have attracted substantial interest for biosensing with optical, electrochemical, and various other means. In particular, given the comparable sizes of nanomaterials and biomolecules, there exists plenty of opportunities to develop functional nanoprobes with biomolecules for highly sensitive and selective biosensing. Over the past decade, numerous nanoprobes have been developed for ultrasensitive bioaffinity sensing of proteins and nucleic acids in both laboratory and clinical applications. In this review, we provide an update on the recent advances in this direction, particularly in the past two years, which reflects new progress since the publication of our last review on the same topic in Chem. Soc. Rev. The types of probes under discussion include: (i) nanoamplifier probes: one nanomaterial loaded with multiple biomolecules; (ii) quantum dots probes: fluorescent nanomaterials with high brightness; (iii) superquenching nanoprobes: fluorescent background suppression; (iv) nanoscale Raman probes: nanoscale surface-enhanced Raman resonance scattering; (v) nanoFETs: nanomaterial-based electrical detection; and (vi) nanoscale enhancers: nanomaterial-induced metal deposition.

  16. Simple approach to study biomolecule adsorption in polymeric microfluidic channels. (United States)

    Gubala, Vladimir; Siegrist, Jonathan; Monaghan, Ruairi; O'Reilly, Brian; Gandhiraman, Ram Prasad; Daniels, Stephen; Williams, David E; Ducrée, Jens


    Herein a simple analytical method is presented for the characterization of biomolecule adsorption on cyclo olefin polymer (COP, trade name: Zeonor(®)) substrates which are widely used in microfluidic lab-on-a-chip devices. These Zeonor(®) substrates do not possess native functional groups for specific reactions with biomolecules. Therefore, depending on the application, such substrates must be functionalized by surface chemistry methods to either enhance or suppress biomolecular adsorption. This work demonstrates a microfluidic method for evaluating the adsorption of antibodies and oligonucleotides surfaces. The method uses centrifugal microfluidic flow-through chips and can easily be implemented using common equipment such as a spin coater. The working principle is very simple. The user adds 40 L of the solution containing the sample to the starting side of a microfluidic channel, where it is moved through by centrifugal force. Some molecules are adsorbed in the channel. The sample is then collected at the other end in a small reservoir and the biomolecule concentration is measured. As a pilot application, we characterized the adsorption of goat anti-human IgG and a 20-mer DNA on Zeonor(®), and on three types of functionalized Zeonor: 3-aminopropyltriethoxysilane (APTES) modified surface with mainly positive charge, negatively charged surface with immobilized bovine serum albumin (BSA), and neutral, hydrogel-like film with polyethylene glycol (PEG) characteristics. This simple analytical approach adds to the fundamental understanding of the interaction forces in real, microfluidic systems. This method provides a straightforward and rapid way to screen surface compositions and chemistry, and relate these to their effects on the sensitivity and resistance to non-specific binding of bioassays using them. In an additional set of experiments, the surface area of the channels in this universal microfluidic chip was increased by precision milling of microscale

  17. Design considerations in the use of stainless steel for vacuum and cryogenic equipment

    CERN Document Server

    Geyari, C


    The properties making austenitic stainless steel a preferred material for the construction of high vacuum equipment are reviewed. Best results are obtained if attention is paid to the improvement of welding properties, particularly with a view to prevent intercrystalline disintegration. A review of mechanical properties, the effect of cold working and cryogenic temperatures on the strength and magnetic characteristics of stainless steel is given. During material selection for very high vacuum, attention must be paid to the porosity problem. A practical example shows the application of these considerations to the choice of materials for the CERN-ISR intersecting storage rings. (19 refs).

  18. Safety Design of Military Electronic Equipment%军用电子设备安全性设计

    Institute of Scientific and Technical Information of China (English)



    Safety is attracting more and more attention as an important part of quality characteristics for weapons and equipment. Due to the more complex composition structure,the higher automatic level and the intercepted probability of electromagnetic wave produced in operation,the possibility of safety accidents occurrence on mili-tary electronic equipment is becoming higher and higher. This paper introduces the safety concept and empha-sizes the functions of safety.According to the requirements of GJB and the practice experience of military elec-tronic equipment,it analyzes the qualitative and quantitative requirements for safety design,proposes the main contents,finally discusses the concrete measures of safety design for personnel,equipment and electromagnetic information for military electronic equipment in detail. The methods presented herein can be useful for those who are engaged in designing,managing and maintaining in this field.%  安全性作为武器装备质量特性中的一项重要内容越来越受到人们的重视。由于组成结构越来越复杂、自动化程度越来越高以及工作时产生的电磁波可能被截获等原因,军用电子设备发生安全事故的可能性越来越大。介绍了安全性的定义,强调了安全性的作用。结合国军标要求和军用电子设备的使用实际,分析了设备安全性设计的定性、定量要求,提出了安全性设计的主要内容,详细讨论了军用电子设备人员、设备的安全性设计和电磁信息安全设计的具体措施,可供该领域从事设计、管理和维修的人员借鉴。

  19. Design, Development, and Evaluation of the Helicopter Sling Load Rapid Aerial Delivery Equipment (United States)


    flight dynamics. 3.1 Concept Generation Several computer aided design (CAD) models were created in SolidWorks ® design software to better...3.2 Finite Element Analysis (FEA) Throughout the design process, the SolidWorks ® system configuration models were processed through FEA software

  20. Engineered collagen hydrogels for the sustained release of biomolecules and imaging agents: promoting the growth of human gingival cells. (United States)

    Choi, Jonghoon; Park, Hoyoung; Kim, Taeho; Jeong, Yoon; Oh, Myoung Hwan; Hyeon, Taeghwan; Gilad, Assaf A; Lee, Kwan Hyi


    We present here the in vitro release profiles of either fluorescently labeled biomolecules or computed tomography contrast nanoagents from engineered collagen hydrogels under physiological conditions. The collagen constructs were designed as potential biocompatible inserts into wounded human gingiva. The collagen hydrogels were fabricated under a variety of conditions in order to optimize the release profile of biomolecules and nanoparticles for the desired duration and amount. The collagen constructs containing biomolecules/nanoconstructs were incubated under physiological conditions (ie, 37°C and 5% CO2) for 24 hours, and the release profile was tuned from 20% to 70% of initially loaded materials by varying the gelation conditions of the collagen constructs. The amounts of released biomolecules and nanoparticles were quantified respectively by measuring the intensity of fluorescence and X-ray scattering. The collagen hydrogel we fabricated may serve as an efficient platform for the controlled release of biomolecules and imaging agents in human gingiva to facilitate the regeneration of oral tissues.

  1. Gerenciando equipes de design interna e externa no processo de desenvolvimento de produtos: caso de duas empresas calçadistas do sul do Brasil.

    Directory of Open Access Journals (Sweden)

    Andréa Capra


    Full Text Available Esse artigo trata da inserção do design no processo de desenvolvimento de produtos em duas empresas da indústria calçadista da região do Vale do Paranhana, no Rio Grande do Sul. Essas empresas podem trabalhar com diferentes tipos de equipes, interna, externa ou mista e, entender as vantagens e desvantagens de cada tipo de equipe é fundamental ao determinar a melhor opção para a empresa. Porém, pouco tem-se estudado buscando essa relação entre equipes de design e desenvolvimento de produtos. Nesse sentido, o objetivo do estudo foi analisar o gerenciamento de equipes de design, interna e externa, no processo de desenvolvimento de produtos de empresas da indústria calçadista. Para isso, um estudo de caso foi realizado através da análise do caso de uma empresa que trabalhava com os dois tipos de equipes. Com a realização desta pesquisa, foi possível estudar a forma de atuação e gerenciamento das equipes interna e externa de design no processo de desenvolvimento de produtos. Os resultados indicam que diferenças nas formas de atuação e gerenciamento de cada equipe estão relacionados com a autonomia decisória conferida para equipe no início do desenvolvimento de novos projetos.

  2. The design of an energy harvesting device for prolonging the working time of DC equipment (United States)

    Wen, Yayuan; Deng, Huaxia; Zhang, Jin; Yu, Liandong


    Energy harvesting (EH) derives from the idea of converting the ambient energy into electric energy, which can solve the problem of DC supply for some electronic equipment. PZT is a typical piezoelectric material of inorganic, which has been developed as EH devices to transfer ambient vibration energy into electric energy. However, these PZT devices require relatively violent excitation, and easy to be fatigue fracture under the resonance condition. In this paper, PVDF, which is a kind of soft piezoelectric polymer, is adopted for developing transducer. The PVDF devices are flexible and have longer life time than PZT devices under the harmonic environment. The EH researches are mainly focused on the development of energy transfer efficiency either by the mechanical structure of transducer or the improvement of circuit. However, the practicality and stability of the EH devices are important in the practical engineering applications. In this paper, a charge amplifier is introduced in the circuit in order to guarantee the stability of the battery charging under small ambient vibration conditions. The model of the mechanical structure of PVDF and the electric performance of circuit are developed. The experimental results and simulation show that the stability of battery charging is improved and the working time of DC equipment is prolonged.

  3. The design of equipment for optical power measurement in FSO link beam cross-section (United States)

    Latal, Jan; David, Tomas; Wilfert, Otakar; Kolka, Zdenek; Koudelka, Petr; Hanacek, Frantisek; Vitasek, Jan; Siska, Petr; Skapa, Jan; Vasinek, Vladimir


    The free space optical links have found their major application in today's technological society. The demand for quality broadband is a must for all types of end users in these times. Because of the large jamming from wireless radio networks in non-licensed ISM bands, the free space optical links provide bridging of some densely populated urban areas. Their advantage is the high transmission rate for relatively long distances. However, the disadvantage is the dependence of free space optical links on atmospheric influences. Aired collimated optical beam passes through the atmospheric transmission environment and by its influence cause the deformation of the optical beam. Author's team decided to construct a special measuring device for measurement of optical power in FSO link beam cross-section. The equipment is mobile and can be rearranged and adjust according to the given location and placement of the FSO link at any time. The article describes the individual structural elements of the measuring equipment, its controlling and application for evaluation and adjustment of measuring steps. The graphs from optical power measurements in the beam cross-section of professional FSO links are presented at the end.

  4. Biomolecule-coated metal nanoparticles on titanium. (United States)

    Christensen, Stephen L; Chatt, Amares; Zhang, Peng


    Immobilizations of nanoparticles and biomolecules on biocompatible substrates such as titanium are two promising approaches to bringing new functionalities to Ti-based biomaterials. Herein, we used a variety of X-ray spectroscopic techniques to study and better understand metal-thiolate interactions in biofunctionalized metal nanoparticle systems supported on Ti substrates. Using a facile one-step procedure, a series of Au nanoparticle samples with varied biomolecule coatings ((2-mercatopropionyl)glycine (MPG) and bovine serum albumin (BSA)) and biomolecule concentrations are prepared. Ag and Pd systems are also studied to observe change with varying metal composition. The structure and properties of these biomolecule-coated nanoparticles are investigated with scanning electron microscopy (SEM) and element-specific X-ray techniques, including extended X-ray absorption fine structure (Au L(3)-edge), X-ray absorption near-edge structure (Au L(3), Ag L(3), Pd L(3), and S K-edge), and X-ray photoelectron spectroscopy (Au 4f, Ag 3d, Pd 3d, and S 2p core level). It was found that, by comparison of SEM and X-ray spectroscopy results, the coating of metal nanoparticles with varying model biomolecule systems can have a significant effect on both surface coverage and organization. This work offers a facile chemical method for bio- and nanofunctionalization of Ti substrates as well as provides a physical picture of the structure and bonding of biocoated metal nanoparticles, which may lead to useful applications in orthopedics and biomedicine.

  5. Generating multiplex gradients of biomolecules for controlling cellular adhesion in parallel microfluidic channels. (United States)

    Didar, Tohid Fatanat; Tabrizian, Maryam


    Here we present a microfluidic platform to generate multiplex gradients of biomolecules within parallel microfluidic channels, in which a range of multiplex concentration gradients with different profile shapes are simultaneously produced. Nonlinear polynomial gradients were also generated using this device. The gradient generation principle is based on implementing parrallel channels with each providing a different hydrodynamic resistance. The generated biomolecule gradients were then covalently functionalized onto the microchannel surfaces. Surface gradients along the channel width were a result of covalent attachments of biomolecules to the surface, which remained functional under high shear stresses (50 dyn/cm(2)). An IgG antibody conjugated to three different fluorescence dyes (FITC, Cy5 and Cy3) was used to demonstrate the resulting multiplex concentration gradients of biomolecules. The device enabled generation of gradients with up to three different biomolecules in each channel with varying concentration profiles. We were also able to produce 2-dimensional gradients in which biomolecules were distributed along the length and width of the channel. To demonstrate the applicability of the developed design, three different multiplex concentration gradients of REDV and KRSR peptides were patterned along the width of three parallel channels and adhesion of primary human umbilical vein endothelial cell (HUVEC) in each channel was subsequently investigated using a single chip.

  6. Thermal Design of Medical Ultrasound Diagnostic Equipment%医用超声诊断设备的热设计

    Institute of Scientific and Technical Information of China (English)

    许奕瀚; 王贤凯; 杨金耀; 林伟杰


      With the expansion of clinical application area, the performance and function of medical ultrasound diagnostic equipment are gradually improved. Meanwhile, the demand for input power also increases which resulting increased heat. The influence of increased heat on the thermal reliability cannot be ignored. This paper describes the demand for thermal design based on the basic theory of thermal design and presents some examples to introduce the thermal design and thermal analysis method of general ultrasound diagnostic equipment.%  随着医用超声诊断设备临床应用领域的不断拓展,其性能、功能逐步提高的同时,对输入功率的需求也在增大,由此带来的热能增大对设备的可靠性产生了不可忽视的影响。本文从热设计的基本理论出发,介绍了热设计的需求,通过实例介绍了一般超声诊断设备的热设计和热分析方法。

  7. 论陈化库设计与设备选择%Design of aging silo and choice of equipment

    Institute of Scientific and Technical Information of China (English)



      重点介绍了陈化库设计需要考虑的因素以及库容量的计算;如何选择陈化设备更合理、更经济,为企业确定陈化方案提供参考。%The design factors of aging silo and the calculation of silo volume, and how to choose the more reasonable aging equipment are described in the paper, which provides the reference for the enterprise to determine the aging scheme.

  8. 电子设备的接地和接地设计%Ground and Ground Design for Electronic Equipment

    Institute of Scientific and Technical Information of China (English)



    从电子设备的安全性和可靠性出发,论述了接地的重要性,并从接地类型、地线作用、接地设计等几方面介绍了有关接地技术的理论及应用。%This essay from electronic equipment of safe and reliability Start, Expound ground of significant, And from ground trpy. ground Line action. Design for ground etc. Servile aspects to introduce have Ground technique of basic knowledge. Basic theory and basic put to use.

  9. Erosion of wind turbine blade coatings - Design and analysis of jet-based laboratory equipment for performance evaluation

    DEFF Research Database (Denmark)

    Zhang, Shizhong; Dam-Johansen, Kim; Nørkjær, Sten


    of the blades in such equipment. To reduce expensive blade maintenance repairs and to avoid out-of-service periods, energy-absorbing blade coatings are required to protect rotor blades from rain erosion. In this work we describe the design, construction and evaluation of a laboratory setup for fast screening...... of up to 22 coating samples that is based on water jet slugs. Our objective is to study the effect of the parameters involved in the rain erosion process and to correlate our experimental results with data obtained with the complex and expensive whirling arm rig, which has become the industry standard...

  10. Impact of the Cooling Equipment on the Key Design Parameters of a Core-Form Power Transformer (United States)

    Orosz, Tamás; Tamus, Zoltán Ádám


    The first step in the transformer design process is to find the active part's key design parameters. This is a non-linear mathematical optimisation task, which becomes more complex if the economic conditions are considered by the capitalisation of the losses. Geometric programming combined with the method of branch and bound can be an effective and accurate tool for this task even in the case of core-form power transformers, when formulating the short-circuit impedance in the required form is problematic. Most of the preliminary design methods consider only the active part of the transformer and the capitalised costs in order to determine the optimal key design parameters. In this paper, an extension of this meta-heuristic transformer optimisation model, which takes the cost of the insulating oil and the cooling equipment into consideration, is presented. Moreover, the impact of the new variables on the optimal key design parameters of a transformer design is examined and compared with the previous algorithm in two different economic scenarios. Significant difference can be found between the optimal set of key-design parameters if these new factors are considered.

  11. Integrated Grease-pouring System Design and Reliability Assessment of Engineering Equipment

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xing; WANG Jian; DAI Ling; YANG Cheng-xian


    Because of poor lubrication,the abrasion of the engineering equipment's key connection position(the hinge joint and so on)has become the major factor that affects the mechanical benefit.The excavator WY60 is taken as an example in this paper.The automatic grease-pouring,the working state test,and the alarm for fault of every lubricating point on the machine is accomplished by the control sub-system,the lubricating system and other correlated components.The principle and functions of the system are described.The work condition is analyzed.Finally,we present the plan of the software and the hardware of the system,and.set up thefuzry ossessment model of reliability of the system.

  12. Design and Operation of Equipment to Detect and Remove Water within Used Nuclear Fuel Storage Bottles

    Energy Technology Data Exchange (ETDEWEB)

    C.C. Baker; T.M. Pfeiffer; J.C. Price


    Inspection and drying equipment has been implemented in a hot cell to address the inadvertent ingress of water into used nuclear fuel storage bottles. Operated with telemanipulators, the system holds up to two fuel bottles and allows their threaded openings to be connected to pressure transducers and a vacuum pump. A prescribed pressure rebound test is used to diagnose the presence of moisture. Bottles found to contain moisture are dried by vaporization. The drying process is accelerated by the application of heat and vacuum. These techniques detect and remove virtually all free water (even water contained in a debris bed) while leaving behind most, if not all, particulates. The extracted water vapour passes through a thermoelectric cooler where it is condensed back to the liquid phase for collection. Fuel bottles are verified to be dry by passing the pressure rebound test.

  13. Biomolecules for removal of heavy metal. (United States)

    Singh, Namita Ashish


    Heavy metals are natural constituents of the earth's crust, but some heavy metals like cadmium, lead, mercury, arsenic etc. are injurious to living organisms at higher concentration. Nowadays, anthropogenic activities have altered geochemical cycles and biochemical balance of heavy metals. Biomolecules are used nowadays for removal of heavy metals compared to other synthetic biosorbents due to their environmental friendly nature and cost effectiveness. The goal of this work is to review research work and patents related to adsorption through biomolecules like polysaccharides, polypeptides, lignin etc. and bio-sorption by biological material that are used for heavy metal removal. Biomolecules are cost effective and there have been significant progresses in the remediation of heavy metals but, still there are some problems that need to be rectified for its application at industrial processes.

  14. On the thermodynamics of biomolecule surface transformations. (United States)

    Federici, Stefania; Oliviero, Giulio; Maiolo, Daniele; Depero, Laura E; Colombo, Italo; Bergese, Paolo


    Biological surface science is receiving great and renewed attention owing the rising interest in applications of nanoscience and nanotechnology to biological systems, with horizons that range from nanomedicine and biomimetic photosynthesis to the unexpected effects of nanomaterials on health and environment. Biomolecule surface transformations are among the fundamental aspects of the field that remain elusive so far and urgently need to be understood to further the field. Our recent findings indicate that surface thermodynamics can give a substantial contribution toward this challenging goal. In the first part of the article, we show that biomolecule surface transformations can be framed by a general and simple thermodynamic model. Then, we explore its effectiveness by addressing some typical cases, including ligand-receptor surface binding, protein thin film machines, nanomechanical aspects of the biomolecule-nanoparticle interface and nanomechanical biosensors.

  15. Simple approach to study biomolecule adsorption in polymeric microfluidic channels

    Energy Technology Data Exchange (ETDEWEB)

    Gubala, Vladimir, E-mail: [Biomedical Diagnostics Institute (BDI), National Centre for Sensor Research (NCSR), Dublin City University, Dublin 9 (Ireland); Medway School of Pharmacy, University of Kent, Central Avenue, Anson 120, Chatham Maritime, Kent ME4 4TB (United Kingdom); Siegrist, Jonathan; Monaghan, Ruairi; O' Reilly, Brian; Gandhiraman, Ram Prasad [Biomedical Diagnostics Institute (BDI), National Centre for Sensor Research (NCSR), Dublin City University, Dublin 9 (Ireland); Daniels, Stephen [Biomedical Diagnostics Institute (BDI), National Centre for Sensor Research (NCSR), Dublin City University, Dublin 9 (Ireland); National Centre for Plasma Science and Technology (NCPST), Dublin City University, Dublin 9 (Ireland); Williams, David E. [Biomedical Diagnostics Institute (BDI), National Centre for Sensor Research (NCSR), Dublin City University, Dublin 9 (Ireland); MacDiarmid Institute for Advanced Materials and Nanotechnology, School of Chemical Sciences, University of Auckland, Auckland 1142 (New Zealand); Ducree, Jens [Biomedical Diagnostics Institute (BDI), National Centre for Sensor Research (NCSR), Dublin City University, Dublin 9 (Ireland)


    Highlights: Black-Right-Pointing-Pointer A simple tool to assess biomolecule adsorption onto the surfaces of microchannels. Black-Right-Pointing-Pointer Development for dilution by surface-adsorption based depletion of protein samples. Black-Right-Pointing-Pointer It can easily be done using a readily available apparatus like a spin-coater. Black-Right-Pointing-Pointer The assessment tool is facile and quantitative. Black-Right-Pointing-Pointer Straightforward comparison of different surface chemistries. - Abstract: Herein a simple analytical method is presented for the characterization of biomolecule adsorption on cyclo olefin polymer (COP, trade name: Zeonor{sup Registered-Sign }) substrates which are widely used in microfluidic lab-on-a-chip devices. These Zeonor{sup Registered-Sign} substrates do not possess native functional groups for specific reactions with biomolecules. Therefore, depending on the application, such substrates must be functionalized by surface chemistry methods to either enhance or suppress biomolecular adsorption. This work demonstrates a microfluidic method for evaluating the adsorption of antibodies and oligonucleotides surfaces. The method uses centrifugal microfluidic flow-through chips and can easily be implemented using common equipment such as a spin coater. The working principle is very simple. The user adds 40 L of the solution containing the sample to the starting side of a microfluidic channel, where it is moved through by centrifugal force. Some molecules are adsorbed in the channel. The sample is then collected at the other end in a small reservoir and the biomolecule concentration is measured. As a pilot application, we characterized the adsorption of goat anti-human IgG and a 20-mer DNA on Zeonor{sup Registered-Sign }, and on three types of functionalized Zeonor: 3-aminopropyltriethoxysilane (APTES) modified surface with mainly positive charge, negatively charged surface with immobilized bovine serum albumin (BSA), and

  16. Microorganisms and biomolecules in space hard environment (United States)

    Horneck, G.


    Microorganisms and biomolecules exposed to space vacuum and to different intensities of selected wavelengths of solar ultraviolet radiation is studied. The influence of these factors, applied singly or simultaneously, on the integrity of microbial systems and biomolecules is measured. Specifically, this experiment will study in Bacillus subtilis spores (1) disturbances in subsequent germination, outgrowth, and colony formation; (2) photochemical reactions of the DNA and protein in vivo and in vitro and their role in biological injury; and (3) the efficiency of repair processes in these events.

  17. Design of roll-to-roll printing equipment with multiple printing methods for multi-layer printing. (United States)

    Kim, Chung Hwan; Jo, Jeongdai; Lee, Seung-Hyun


    In this paper, a novel design concept for roll-to-roll printing equipment used for manufacturing printed electronic devices by multi-layer printing is presented. The roll-to-roll printing system mainly consists of printing units for patterning the circuits, tension control components such as feeders, dancers, load cells, register measurement and control units, and the drying units. It has three printing units which allow switching among the gravure, gravure-offset, and flexo printing methods by changing the web path and the placements of the cylinders. Therefore, depending on the application devices and the corresponding inks used, each printing unit can be easily adjusted to the required printing method. The appropriate printing method can be chosen depending on the desired printing properties such as thickness, roughness, and printing quality. To provide an example of the application of the designed printing equipment, we present the results of printing tests showing the variations in the printing properties of the ink for different printing methods.

  18. Design and adaptation of miniaturized electrochemical devices integrating carbon nanotube-based sensors to commercial CE equipment. (United States)

    Arribas, Alberto Sánchez; Moreno, Mónica; Bermejo, Esperanza; Angeles Lorenzo, M; Zapardiel, Antonio; Chicharro, Manuel


    The design of new electrochemical devices integrating carbon nanotube sensors and their adaptation to commercial CE equipments are described. One of these designs was made for using commercial screen-printed electrodes, whereas the second was projected for coupling commercial glassy carbon electrodes. The electrochemical characterization of these devices revealed that their hydrodynamic behaviour is strongly influenced by the electrode modification with multi-wall carbon nanotubes that provided faster and/or more sensitive signals. The analytical applicability of these devices was illustrated for the CZE separation of chlorinated phenols and the MEKC separation of endocrine disruptors, where the use of carbon nanotube sensors has proved to be advantageous when compared with unmodified ones, with good electrocatalytic properties combined with acceptable background currents and a remarkable resistance to passivation.

  19. Evaluating performance of containment equipment designed for handling manufactured nanomaterials by use of nanoparticle tracer (United States)

    Artous, Sébastien; Bombardier, Pierre; Derrough, Samir; Locatelli, Dominique; Nobile, Pierre; Durand, Catherine


    The implementation in many products of manufactured nanoparticles is in strong growth and raises new questions. For this purpose, the CEA - NanoSafety Platform is developing various research topics for health and safety, environment and nanoparticles exposure in professional activities. The working group Nano-CERT/MTD, driven by INERIS, federates actors of the sector: experts, research organizations, industrial users and manufacturers of collective protection. The main activity of this group is to establish specific guidelines or a voluntary certification of collective protection, at a national scale, but with the possibility of a further extension at an European level. The group aims to establish an experimental protocol of certification to characterize collective protections for workers faced with nanomaterials potential exposure. The NanoSafety Platform provides in this presentation a method of collective protection characterization based on the developments in nanoparticles metrology and on the study of existing standards and practices in related areas (chemicals, dust, microbiological and nuclear). This study presents the results obtained during the experimental characterization of a potential nanoparticles transfer in a prototype laboratory fume hood by the use of a particulate tracer of sodium-fluorescein. The efficiency of the equipment and more specifically the efficiency of dynamical air barrier is evaluated, with the experimental results, by calculating the backward diffusion coefficient.

  20. A Multidisciplinary, Open Access Platform for Research on Biomolecules. (United States)

    Bähler, Jürg


    I am pleased to introduce Biomolecules, a new journal to report on all aspects of science that focuses on biologically derived substances, from small molecules to complex polymers. Some examples are lipids, carbohydrates, vitamins, hormones, amino acids, nucleotides, peptides, RNA and polysaccharides, but this list is far from exhaustive. Research on biomolecules encompasses multiple fascinating questions. How are biomolecules synthesized and modified? What are their structures and interactions with other biomolecules? How do biomolecules function in biological processes, at the level of organelles, cells, organs, organisms, or even ecosystems? How do biomolecules affect either the organism that produces them or other organisms of the same or different species? How are biomolecules shaped by evolution, and how in turn do they affect cellular phenotypes? What is the systems-level contribution of biomolecules to biological function? [...].

  1. A Multidisciplinary, Open Access Platform for Research on Biomolecules

    Directory of Open Access Journals (Sweden)

    Jürg Bähler


    Full Text Available I am pleased to introduce Biomolecules, a new journal to report on all aspects of science that focuses on biologically derived substances, from small molecules to complex polymers. Some examples are lipids, carbohydrates, vitamins, hormones, amino acids, nucleotides, peptides, RNA and polysaccharides, but this list is far from exhaustive. Research on biomolecules encompasses multiple fascinating questions. How are biomolecules synthesized and modified? What are their structures and interactions with other biomolecules? How do biomolecules function in biological processes, at the level of organelles, cells, organs, organisms, or even ecosystems? How do biomolecules affect either the organism that produces them or other organisms of the same or different species? How are biomolecules shaped by evolution, and how in turn do they affect cellular phenotypes? What is the systems-level contribution of biomolecules to biological function?

  2. Nanofluidic technology for biomolecule applications: a critical review

    NARCIS (Netherlands)

    Napoli, M.; Eijkel, J.C.T.; Pennathur, S.


    In this review, we present nanofluidic phenomena, particularly as they relate to applications involving analysis of biomolecules within nanofabricated devices. The relevant length scales and physical phenomena that govern biomolecule transport and manipulation within nanofabricated nanofluidic devic

  3. Thermal design of a fully equipped solar-powered desert home

    KAUST Repository

    Serag-Eldin, M.A.


    The paper presents a conceptual design and thermodynamic analysis of a solar-powered desert home. The home is airconditioned and provides all modern comforts and facilities. Electrical power, which drives the entire energy system, is generated by roof mounted photovoltaic modules. A detailed dynamic heat transfer analysis is conducted for the building envelope, coupled with a solar radiation model. A dynamic heat balance for a typical Middle-Eastern desert site, reveals that indeed such a design is feasible with present day technology; and should be even more attractive with future advances in technology.

  4. Tuneable graphene nanopores for single biomolecule detection (United States)

    Al-Dirini, Feras; Mohammed, Mahmood A.; Hossain, Md Sharafat; Hossain, Faruque M.; Nirmalathas, Ampalavanapillai; Skafidas, Efstratios


    Solid-state nanopores are promising candidates for next generation DNA and protein sequencing. However, once fabricated, such devices lack tuneability, which greatly restricts their biosensing capabilities. Here we propose a new class of solid-state graphene-based nanopore devices that exhibit a unique capability of self-tuneability, which is used to control their conductance, tuning it to levels comparable to the changes caused by the translocation of a single biomolecule, and hence, enabling high detection sensitivities. Our presented quantum simulation results suggest that the smallest amino acid, glycine, when present in water and in an aqueous saline solution can be detected with high sensitivity, up to a 90% change in conductance. Our results also suggest that passivating the device with nitrogen, making it an n-type device, greatly enhances its sensitivity, and makes it highly sensitive to not only the translocation of a single biomolecule, but more interestingly to intramolecular electrostatics within the biomolecule. Sensitive detection of the carboxyl group within the glycine molecule, which carries a charge equivalent to a single electron, is achieved with a conductance change that reaches as high as 99% when present in an aqueous saline solution. The presented findings suggest that tuneable graphene nanopores, with their capability of probing intramolecular electrostatics, could pave the way towards a new generation of single biomolecule detection devices.

  5. Teaching Computer-Aided Design of Fluid Flow and Heat Transfer Engineering Equipment. (United States)

    Gosman, A. D.; And Others


    Describes a teaching program for fluid mechanics and heat transfer which contains both computer aided learning (CAL) and computer aided design (CAD) components and argues that the understanding of the physical and numerical modeling taught in the CAL course is essential to the proper implementation of CAD. (Author/CMV)

  6. Porous-wall hollow glass microspheres as carriers for biomolecules (United States)

    Li, Shuyi; Dynan, William S; Wicks, George; Serkiz, Steven


    The present invention includes compositions of porous-wall hollow glass microspheres and one or more biomolecules, wherein the one or more biomolecules are positioned within a void location within the hollow glass microsphere, and the use of such compositions for the diagnostic and/or therapeutic delivery of biomolecules.

  7. Design and development of electric vehicle charging station equipped with RFID (United States)

    Panatarani, C.; Murtaddo, D.; Maulana, D. W.; Irawan, S.; Joni, I. M.


    This paper reports the development of electric charging station from distributed renewable for electric vehicle (EV). This designed refer to the input voltage standard of IEC 61851, plugs features of IEC 62196 and standard communication of ISO 15118. The developed electric charging station used microcontroller ATMEGA8535 and RFID as controller and identifier of the EV users, respectively. The charging station successfully developed as desired features for electric vehicle from renewable energy resources grid with solar panel, wind power and batteries storage.

  8. Design Study and Optimization of Irradiation Facilities for Detector and Accelerator Equipment Testing in the SPS North Area at CERN

    CERN Document Server

    Biskup, Bartolomej; Stekl, Ivan

    Due to increasing performance of LHC during the last years, the strong need of new detector and electronic equipment test areas at CERN appeared from user communities. This thesis reports on two test facilities: GIF++ and H4IRRAD. GIF++, an upgrade of GIF facility, is a combined high-intensity gamma and particle beam irradiation facility for testing detectors for LHC. It combines a high-rate 137Cs source, providing photons with energy of 662 keV, together with the high-energy secondary particle beam from SPS. H4IRRAD is a new mixed-field irradiation area, designed for testing LHC electronic equipment for radiation damage effects. In particular, large volume assemblies such as full electronic racks of high current power converters can be tested. The area uses alternatively an attenuated primary 400 GeV/c proton beam from SPS, or a secondary, mainly proton, beam of 280 GeV/c directed towards a copper target. Different shielding layers are used to reproduce a radiation field similar to the LHC “tunnel” and �...

  9. Demonstration Plant Equipment Design and Scale-Up from Pilot Plant of a Leaching and Solvent Extraction Process

    Directory of Open Access Journals (Sweden)

    Fátima Arroyo


    Full Text Available Germanium recovery from coal fly ash by hydrometallurgical procedures was studied at the pilot scale (5 kg of fly ash/h. Results were used to design the equipment of a demonstration-sized plant (200 kg of fly ash/h. The process is based on hydrometallurgical operations: firstly a germanium extraction from fly ash by leaching and a consequent Ge separation from the other elements present in the solution by solvent extraction procedures. Based on the experimental results, mass balances and McCabe-Thiele diagrams were applied to determine the number of steps of the solvent extraction stage. Different arrangements have been studied and a countercurrent process with three steps in extraction and six steps in elution was defined. A residence time of 5 min was fixed in both the extraction and elution stages. Volumetric ratios in extraction and stripping were: aqueous phase/organic phase = 5 and organic phase/stripping phase = 5, so a concentration factor of 25 is achieved. Mixers and decanters were completely defined. The maximum extracted and eluted germanium was estimated and a global efficiency of 94% was achieved. The cost-effectiveness of the equipment was estimated using the Lang factors.

  10. Optimal design of wind power generation equipment%风力发电设备的优化设计

    Institute of Scientific and Technical Information of China (English)



    风能清洁无污染,是人们梦寐以求的新能源。风力发电是一种有效利用风能的新形式,风力发电设备决定着发电效率与发电成本,因此优化发电设备至关重要。通过阐述风力发电基本原理及现代风机的基本组成,提出风力发电设备优化设计的几点思路,为提高风力发电可利用价值提供参考。%Wind power is clean and pollution-free, which is the new energy people want to use. Wind power generation is aneffectivenewformofusingwindenergy,windpowergenerationequipmentdeterminestheefficiencyandcostofpower generation, therefore to optimize power generation equipment is essential. Through elaborating basic composition of wind power generation and basic principles of modern wind turbines, this paper proposed a few ideas on optimal design of wind power generation equipment, which provided reference for increasing its use value.

  11. a New Design for Diamond Window Equipped Paris-Edinburgh — First Tests and Results (United States)

    Burchard, M.; Glasmacher, U. A.; Dedera, S.; Trautmann, C.


    High pressure cells of the Paris-Edinburgh type are important tools for experimental high pressure studies in material science. Although these cells were originally developed for use in neutron diffraction, today they are also applied in alternative experimental high pressure fields. Their main advantages are their small construction size, limited weight and the relative high reachable pressures with a maximized sample volume. The small construction size also results in very good cost efficiency. The major drawback of these cells is that due to their simple squeezer geometry pressure extrapolations are nearly impossible and, hence, the use of internal standard is mandatory. Consequently, the normal use of Paris-Edinburgh cells is mostly limited to experiments with neutron or synchrotron radiation, during which pressure and temperature are determined by using an internal diffraction standard. To overcome this problem, tone may combine the advantages of diamond anvil cells with those of Paris-Edinburgh-cells by integrating diamond windows into the upper and / or the lower anvil. With such a cell it is possible to retrieve pressure and / or temperature data by measuring the shift of Raman bands or fluorescence lines by spectroscopic methods. Several attempts have been made to build such a cell ([1] and Klotz pers. communication) using different window materials. Until now no final setup has been published. We present a new Paris-Edinburgh cell design especially constructed for use within high energy relativistic heavy ion radiation experiments. This design uses conventional diamond anvils as spectroscopic windows in conjunction with specially designed hardened steel anvils. We have performed first feasibility studies up to 2.5 GPa with a standard Raman spectrometer using a special adapted Raman glass fiber probe. We present results of several mechanical test runs and one experiment with relativistic heavy ion radiation at the GSI Helmholtzzentrum f

  12. Climate design of vegetable oil fuels for agricultural equipment; Klimadesign von Pflanzenoelkraftstoffen fuer landwirtschaftliche Maschinen

    Energy Technology Data Exchange (ETDEWEB)

    Stoehr, Michael [B.A.U.M. Consult GmbH, Muenchen (Germany). International and Energy Projects; Pickel, Peter [John Deere European Technology Innovation Center, Kaiserslautern (Germany)


    The use of biofuels in agricultural machinery is an option for complying with climate protection requirements that are presently discussed to be placed on manufacturers of mobile off-road machinery by the European Commission. A mathematical model has been developed that allows calculating greenhouse gas emissions (GHGE) of biofuels for complex production paths in a straightforward, transparent manner and in pattern with the EU's Fuel Quality Directive (FQD). Therewith it has been shown that both rape seed and camelina sativa oil fuels can save more than 60 % GHGE. Key parameters have been identified and rules for a climate design of vegetable oil fuels have been formulated. (orig.)

  13. Electrical insulation design and evaluation of 60 kV prototype condenser cone bushing for the superconducting equipment

    Energy Technology Data Exchange (ETDEWEB)

    Shin, Woo-Ju, E-mail:; Lee, Jong-Geon; Hwang, Jae-Sang; Seong, Jae-Kyu; Lee, Bang-Wook, E-mail:


    Highlights: •The optimum design of condenser cone cryogenic bushing was investigated. •Multi-layer aluminum foils in the bushing insulation body was designed and analyzed. •The optimum electric field distribution was selected by simulation. •The 60 kV FRP condenser cone cryogenic bushing was fabricated and tested. •BIL test corresponding to IEC 60137 was successfully performed for the bushing. -- Abstract: A cryogenic bushing is an essential component to be developed for commercial applications of high voltage (HV) superconducting devices. Due to the steep temperature gradient of the ambient of cryogenic bushing, general gas bushing adopting SF6 gas as an insulating media could not be directly used due to the freezing of SF6 gas. Therefore, condenser type bushing with special material considering cryogenic environment would be better choice for superconducting equipment. Considering these circumstance, we focused on the design of condenser bushing made of fiber reinforced plastic (FRP). In case of the design of the condenser bushing, it is very important to reduce the electric field intensification on the mounted flange part of the cryostat, which is the most vulnerable part of bushings. In this paper, design factors of cryogenic bushing were analyzed, and finally 60 kV condenser bushing was fabricated and tested. In order to achieve optimal electric field configuration, the configuration of condenser cone was determined using 2D electric field simulation results. Based on the experimental and the analytical works, 60 kV FRP condenser bushing was fabricated. Finally, the fabricated condenser bushing has been tested by applying lightning impulse and AC overvoltage test. From the test results, it was possible to get satisfactory results which confirm the design of cryogenic bushing in cryogenic environment.

  14. The Design of Ground Test Equipment on Special Computer%某计算机地面检测设备的设计

    Institute of Scientific and Technical Information of China (English)



    机载电子设备的研制和开发,需要功能完备的地面检测设备。本文简要介绍了某计算机基于微程序的地面检测设备的软硬件设计及其实施途径。%The research of equipment on airplane need ground test equipment which have maturity functions. This paper introduces design of hardware, software of the ground test equipment of the some airplane computer based micro-program, and the method how to carry into execution.

  15. High density wireless EEG prototype: Design and evaluation against reference equipment. (United States)

    Rossi, Stefano; Patki, Shrishail; Passoni, Marco; Perko, Hannes; Gritsch, Gerhard; Ossenblok, Pauly; Yazicioglu, Refet Firat


    A high density wireless electroencephalographic (EEG) platform has been designed. It is able to record up to 64 EEG channels with electrode to tissue impedance (ETI) monitoring. The analog front-end is based on two kinds of low power ASICs implementing the active electrodes and the amplifier. A power efficient compression algorithm enables the use of continuous wireless transmission of data through Bluetooth for real-time monitoring with an overall power consumption of about 350 mW. EEG acquisitions on five subjects (one healthy subject and four patients suffering from epilepsy) have been recorded in parallel with a reference system commonly used in clinical practice and data of the wireless prototype and reference system have been processed with an automatic tool for seizure detection and localization. The false alarm rates (0.1-0.5 events per hour) are comparable between the two system and wireless prototype also detected the seizure correctly and allowed its localization.

  16. The design of the automated control system for warehouse equipment under radio-electronic manufacturing (United States)

    Kapulin, D. V.; Chemidov, I. V.; Kazantsev, M. A.


    In the paper, the aspects of design, development and implementation of the automated control system for warehousing under the manufacturing process of the radio-electronic enterprise JSC «Radiosvyaz» are discussed. The architecture of the automated control system for warehousing proposed in the paper consists of a server which is connected to the physically separated information networks: the network with a database server, which stores information about the orders for picking, and the network with the automated storage and retrieval system. This principle allows implementing the requirements for differentiation of access, ensuring the information safety and security requirements. Also, the efficiency of the developed automated solutions in terms of optimizing the warehouse’s logistic characteristics is researched.

  17. The structure design and performance analysis for damping system of the airborne equipment (United States)

    Zhang, Wei; Wu, Chun-xia; Yan, Cong-lin; Cui, Ding; Ma, She


    Vibration is an important factor that could affect the performance of airbone optical system, the damping device based on the wire-rope vibration isolators was designed in this paper, in which the optical system mounted on the helicopter was taken as an example. The transmissibility of the damping device was about 40% which obtained by finite element method, the transmissibility of the damping device was about 36% which obtained by vibration platform test, the result obtained by finite element method was proved by vibration platform test. The vibration of the optical system could been reduced significantly as a result of the device with good damping effect, thereby the stability of the optical system could be enhanced.

  18. Design and evaluation of a medical teamwork training simulator using consumer-level equipment. (United States)

    Marks, Stefan; Windsor, John A; Wünsche, Burkhard


    Virtual environments (VE) are increasingly used for teamwork training purposes, e.g., for medical teams. One shortcoming is lack of support for nonverbal communication channels, essential for teamwork. We address this issue by using an inexpensive webcam to track the user's head and using that data for controlling avatar head movement, thereby conveying head gestures and adding a nonverbal communication channel. In addition, navigation and orientation within the virtual environment is simplified. We present the design and evaluation of a simulation framework based on a game engine and consumer-level hardware and the results of two user studies showing, among other results, an improvement in the usability of the VE and in the perceived quality of realism and communication within the VE by using head tracking avatar and view control.

  19. Equipment concept design and development plans for microgravity science and applications research on space station: Combustion tunnel, laser diagnostic system, advanced modular furnace, integrated electronics laboratory (United States)

    Uhran, M. L.; Youngblood, W. W.; Georgekutty, T.; Fiske, M. R.; Wear, W. O.


    Taking advantage of the microgravity environment of space NASA has initiated the preliminary design of a permanently manned space station that will support technological advances in process science and stimulate the development of new and improved materials having applications across the commercial spectrum. Previous studies have been performed to define from the researcher's perspective, the requirements for laboratory equipment to accommodate microgravity experiments on the space station. Functional requirements for the identified experimental apparatus and support equipment were determined. From these hardware requirements, several items were selected for concept designs and subsequent formulation of development plans. This report documents the concept designs and development plans for two items of experiment apparatus - the Combustion Tunnel and the Advanced Modular Furnace, and two items of support equipment the Laser Diagnostic System and the Integrated Electronics Laboratory. For each concept design, key technology developments were identified that are required to enable or enhance the development of the respective hardware.

  20. Biomolecule delivery to engineer the cellular microenvironment for regenerative medicine. (United States)

    Bishop, Corey J; Kim, Jayoung; Green, Jordan J


    To realize the potential of regenerative medicine, controlling the delivery of biomolecules in the cellular microenvironment is important as these factors control cell fate. Controlled delivery for tissue engineering and regenerative medicine often requires bioengineered materials and cells capable of spatiotemporal modulation of biomolecule release and presentation. This review discusses biomolecule delivery from the outside of the cell inwards through the delivery of soluble and insoluble biomolecules as well as from the inside of the cell outwards through gene transfer. Ex vivo and in vivo therapeutic strategies are discussed, as well as combination delivery of biomolecules, scaffolds, and cells. Various applications in regenerative medicine are highlighted including bone tissue engineering and wound healing.

  1. Mission design study of an RTG powered, ion engine equipped interstellar spacecraft (United States)

    Fogel, Joshua A.

    This research explores a variety of mission and system architectures for an unmanned Interstellar Precursor Mission (IPM) spacecraft with a Radioisotope Thermoelectric Generator (RTG) powered Ion Engine using Xenon propellant, traveling on a (direct) ballistic escape trajectory to the undisturbed Interstellar Medium (˜200 AU). The main goal of this work was to determine the relationship between the propulsion system design parameters and the ensuing escape trajectory. To do this, an orbit simulator was created in Matlab using a fourth order Runge-Kutta numerical integration method to propagate the thrusting spacecraft's trajectory through time. The accelerations due to the Sun's gravity and the Ion Engine thrust were modeled separately and then combined into a single total acceleration vector at each time step, with the thrust direction assumed to be in the direction of the spacecraft's instantaneous velocity vector. The propellant of the thruster was also designed to be completely consumed by the time of engine cut-off (ECO), meaning a constant propellant mass flow rate. Simulations were run for burn times of 5, 10 & 15 years, with heliocentric launch velocities of 0, 5, 7, 10 & 12 km/sec from a circular 1 AU Earth orbit, and with RTG supplied engine input powers of 1000, 1500 & 2000 W. A total of 45 simulations were run for the circular 1 AU case, as well as additional comparison simulations for launches from an elliptical Earth orbit at perihelion and aphelion. The results of these simulations yielded many interesting results on the total fly-out times to 200 AU, which ranged dramatically from ˜35 to ˜140 years depending on the propulsion system settings and orbital initial conditions, as well as descriptions of the ECO distances from the Sun for each mission. The simulations also revealed the inherent gravitational maneuver inefficiency felt by all low thrust spacecraft, which becomes more apparent under certain conditions. Relations between launch velocity

  2. Monitoring the synthesis of biomolecules using mass spectrometry. (United States)

    Miyagi, Masaru; Kasumov, Takhar


    The controlled and selective synthesis/clearance of biomolecules is critical for most cellular processes. In most high-throughput 'omics' studies, we measure the static quantities of only one class of biomolecules (e.g. DNA, mRNA, proteins or metabolites). It is, however, important to recognize that biological systems are highly dynamic in which biomolecules are continuously renewed and different classes of biomolecules interact and affect each other's production/clearance. Therefore, it is necessary to measure the turnover of diverse classes of biomolecules to understand the dynamic nature of biological systems. Herein, we explain why the kinetic analysis of a diverse range of biomolecules is important and how such an analysis can be done. We argue that heavy water ((2)H2O) could be a universal tracer for monitoring the synthesis of biomolecules on a global scale.This article is part of the themed issue 'Quantitative mass spectrometry'.

  3. Dynamic modelling and active vibration controller design for a cylindrical shell equipped with piezoelectric sensors and actuators (United States)

    Kwak, Moon K.; Heo, Seok; Jeong, Moonsan


    This paper is concerned with the dynamic modelling, active vibration controller design and experiments for a cylindrical shell equipped with piezoelectric sensors and actuators. The dynamic model was derived by using Rayleigh-Ritz method based on the Donnel-Mushtari shell theory. The actuator equations which relate the applied voltages to the generalized force and sensor equations which relate the generalized displacements to the sensor output voltages for the piezoelectric wafer were derived based on the pin-force model. The equations of motion along with the piezoelectric sensor equations were then reduced to modal forms considering the modes of interest. An aluminium shell was fabricated to demonstrate the effectiveness of the modelling and control techniques. The boundary conditions at both ends of the shell were assumed to be a shear diaphragm in the numerical analysis. Theoretical natural frequencies of the aluminium shell were then calculated and compared to experimental result. They were in good agreement with experimental result for the first two free-vibration modes. The multi-input and multi-output positive position feedback controller, which can cope with the first two vibration modes, was designed based on the block-inverse theory and was implemented digitally using the DSP board. The experimental results showed that vibrations of the cylindrical shell can be successfully suppressed by the piezoelectric actuator and the proposed controller.

  4. Design of Hoisting Equipment for Transit Theodolite%经纬仪吊装机构设计

    Institute of Scientific and Technical Information of China (English)

    徐燕; 佘晶


    介绍了经纬仪吊装机构设计过程,包括吊装机构的方案选择、内外管的强度校核以及手拉葫芦及轴承的选型。试验证明,该吊装机构能满足经纬仪的出入舱要求,安全可靠,操作简单快捷。与随车吊相比,该吊装机构加工成本低。%The design method and the manufacturing process of hoisting equipment for the transit theodolite are introduced. The design plan selection, the strength check of the inside and outside tubes, the model selections of the chain block and the bearing are presented, respectively. Exper- imental results show that the lifting mechanism can meet the entrance and exit requirements for the transit theodolite. The system has advantages of safe and reliable, easy operation and conve- nient process. In addition, the cost is fewer than that of the truck mounted crane.

  5. The Design of Medical Equipment Management System Based on the Development of Microsoft Access%基于Access开发的医疗设备管理系统

    Institute of Scientific and Technical Information of China (English)



    According to the specific needs of digital management for medical equipments, the equipment department in our hospital uses Microsoft Access developing a set of high efficiency of medical equipment management system, which makes the medical equipment management standardized and systematic. This paper introduces the basic knowledge of Access, and explains the design process of the system in detail.%我院设备科根据对医疗设备数字化管理的具体需求,使用Microsoft Access开发了医疗设备管理系统,使医疗设备管理更加规范化、系统化.本文介绍了Access的基本知识,并详解了此系统的设计与实现过程.

  6. 基于Django框架的设备管理系统的设计与实现%Django Framework Based Equipment Management System Design and Implementation

    Institute of Scientific and Technical Information of China (English)



    该文通过分析当前设备管理系统的应用场景和使用情况,将设备管理系统分解为若干模块,以用户角色为基础设计了一套完整的电子化设备管理系统。利用Python语言设计与开发了基于Django框架的设备管理系统。%This paper analyzes the current device management system applications and usage scenarios, the equipment manage-ment system is decomposed into several modules, user-role-based design of a complete set of electronic equipment management system. Design and development using the Python language Django framework based equipment management system.

  7. Design of Hydraulic Protection Equipment Served the Disabled Weight-lifting%残疾人举重液压伺服保护装置设计

    Institute of Scientific and Technical Information of China (English)



    A kind of hydraulic protection equipment served the disabled weight⁃lifting was designed. The equipment consisted of guide rail, hydraulic following unit and hydraulic lifting unit. It mainly depended on hydraulic servo theory to work. The technical pro⁃posal and the model designs of the core structure of the equipment were stated.%设计一种残疾人举重保护装置,该装置由导轨、液压跟随单元、液压托举单元等三部分组成,主要依据液压伺服原理工作。阐述了该装置的技术方案,并对装置的核心结构做了选型设计。

  8. 10 CFR 50.34a - Design objectives for equipment to control releases of radioactive material in effluents-nuclear... (United States)


    ... radioactive material in effluents-nuclear power reactors. 50.34a Section 50.34a Energy NUCLEAR REGULATORY... objectives for equipment to control releases of radioactive material in effluents—nuclear power reactors. (a... equipment to be installed to maintain control over radioactive materials in gaseous and liquid...

  9. 42 CFR 421.210 - Designations of regional carriers to process claims for durable medical equipment, prosthetics... (United States)


    ... for durable medical equipment, prosthetics, orthotics and supplies. 421.210 Section 421.210 Public... claims for durable medical equipment, prosthetics, orthotics and supplies. (a) Basis. This section is..., prosthetics, orthotics, and other supplies (DMEPOS). This authority has been delegated to CMS. (b) Types...

  10. Design, Fabrication and pseudo Field Evaluation of a Sugar Beet Crust Breaker and Weeding Unit Equipped with an Infrared Sensor

    Directory of Open Access Journals (Sweden)

    E Chaligar


    Full Text Available In conventional cultivation of sugar beet the weeding and crust breaking are mostly performed manually. The objectives of this research were to design, fabricate and evaluate a soil crust breaker and weeding implement equipped with a detecting sensor. Each unit consisted of a distance detecting sensor and a hydro-motor for driving the blades and pneumatic valves for moving the blades. The hydro-motor was activated by the sensors. To avoid damaging the plants, a command signal was sent to the pneumatic valves to move the blades up and down and pass safely. Three configurations of cutting blades were considered which could be mounted to the crust breaker. The field evaluation was conducted with two tractor forward speeds (0.4 and 1 km h-1 and four plant-to-plant spacings within rows (20, 25, 30 and 40 cm all with three different blade shapes. For field evaluation split plot experiments arranged in a completely randomized block design with three replications. The number of plants damaged (evaluated only for four-lobe blades and size of broken crusts (evaluated for all blade shapes for various treatments were recorded and compared. The results of analysis indicated that the higher the inter-row spacing the lower the injury to the plant. The highest and the least damage to the plants occurred for within-row plant spacings of 20 and 40 cm, respectively. The forward speed was also significantly affected the percent of plant damaged. The forward speed of 1 km h-1 at 20 cm spacing had the most (59% and the speed of 0.40 km h-1 at 40 cm spacing had the least effect (3.3% on the percent of plant damage. The two-lobe blade could result in the maximum surface area broken.

  11. Porous solid ion exchange wafer for immobilizing biomolecules (United States)

    Arora, Michelle B.; Hestekin, Jamie A.; Lin, YuPo J.; St. Martin, Edward J.; Snyder, Seth W.


    A porous solid ion exchange wafer having a combination of a biomolecule capture-resin and an ion-exchange resin forming a charged capture resin within said wafer. Also disclosed is a porous solid ion exchange wafer having a combination of a biomolecule capture-resin and an ion-exchange resin forming a charged capture resin within said wafer containing a biomolecule with a tag. A separate bioreactor is also disclosed incorporating the wafer described above.

  12. Site-Specific Biomolecule Labeling with Gold Clusters


    Ackerson, Christopher J.; Powell, Richard D.; Hainfeld, James F.


    Site-specific labeling of biomolecules in vitro with gold clusters can enhance the information content of electron cryomicroscopy experiments. This chapter provides a practical overview of well-established techniques for forming biomolecule/gold cluster conjugates. Three bioconjugation chemistries are covered: Linker-mediated bioconjugation, direct gold–biomolecule bonding, and coordination-mediated bonding of nickel(II) nitrilotriacetic acid (NTA)-derivatized gold clusters to polyhistidine (...

  13. Immobilization of biomolecules on semiconductor surfaces (United States)

    Joensson, U.; Malmqvist, M.; Nilsson, H.; Olofsson, G.; Roennberg, I.


    A reproducible, stable and functional introduction of reactive groups on oxide covered silicon surfaces used in chemically sensitive field effect transistors and optical methods based on light reflection is described. Biomolecules, such as antibodies, antigens and enzymes, were covalently attached to the surface modified silicon via a thiol disulfide exchange reaction. The immobilization technique eliminates the risk of crosslinking and homopolymerization, giving monolayer coverage in close contact with the surface. The technique was used for immobilized protein A and interaction of such surfaces with immunoglobulins. The result was evaluated by in situ ellipsometry, which gives the amount of immobilized and interacting material on the surfaces.

  14. Design and fabrication of equipment and devices for a Radiotherapy department; Diseno y fabricacion de equipos y aditamentos para un departamento de Radioterapia

    Energy Technology Data Exchange (ETDEWEB)

    Picon, C.L.; Zaharia B, M. [Departamento de Radioterapia. Instituto de Enfermedades Neoplasicas, Av. Angamos Este 2520, Lima (Peru)


    The objective of this work is to present the possibility to design and construct utilizing local technology, a series of equipment and devices necessaries for a routine in a Radiotherapy Department with typical budget in the Latin-American hospitals. (Author)

  15. Cell-selective metabolic labeling of biomolecules with bioorthogonal functionalities. (United States)

    Xie, Ran; Hong, Senlian; Chen, Xing


    Metabolic labeling of biomolecules with bioorthogonal functionalities enables visualization, enrichment, and analysis of the biomolecules of interest in their physiological environments. This versatile strategy has found utility in probing various classes of biomolecules in a broad range of biological processes. On the other hand, metabolic labeling is nonselective with respect to cell type, which imposes limitations for studies performed in complex biological systems. Herein, we review the recent methodological developments aiming to endow metabolic labeling strategies with cell-type selectivity. The cell-selective metabolic labeling strategies have emerged from protein and glycan labeling. We envision that these strategies can be readily extended to labeling of other classes of biomolecules.

  16. Technology Equipment Rooms. (United States)

    Day, C. William


    Examines telecommunications equipment room design features that allow for growth and can accommodate numerous equipment replacements and upgrades with minimal service disruption and with minimal cost. Considerations involving the central hub, power and lighting needs, air conditioning, and fire protection are discussed. (GR)

  17. 46 CFR 153.486 - Design and equipment for removing NLS residue by ventilation: Categories A, B, C, and D. (United States)


    ... (a) of this section the ship must have— (1) Portable forced air ventilating equipment fitting the... allows a fan or air supply to be connected to the hose connections for the tank at the manifold....


    Energy Technology Data Exchange (ETDEWEB)

    J. Steinhoff


    The objective and the scope of this document are to list and briefly describe the major mobile equipment necessary for waste package (WP) retrieval from the proposed subsurface nuclear waste repository at Yucca Mountain. Primary performance characteristics and some specialized design features of the equipment are explained and summarized in the individual subsections of this document. There are no quality assurance requirements or QA controls in this document. Retrieval under normal conditions is accomplished with the same fleet of equipment as is used for emplacement. Descriptions of equipment used for retrieval under normal conditions is found in Emplacement Equipment Descriptions, DI: BCAF00000-01717-5705-00002 (a document in progress). Equipment used for retrieval under abnormal conditions is addressed in this document and consists of the following: (1) Inclined Plane Hauler; (2) Bottom Lift Transporter; (3) Load Haul Dump (LHD) Loader; (4) Heavy Duty Forklift for Emplacement Drifts; (5) Covered Shuttle Car; (6) Multipurpose Vehicle; and (7) Scaler.

  19. Recent advances in the designing and the equipment of high activity laboratories; Progres recents dans la conception et l'equipement des laboratoires de haute activite

    Energy Technology Data Exchange (ETDEWEB)

    Bazire, R.; Duhamel, F. [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires


    The authors described the general principles governing the design of a laboratory for experimenting and handling radioactive substances. The difficulties encountered are of two types: 1) those due to the dangers of external irradiation; 2) those due to the dangers of internal contamination. As an example, the authors describe the French achievements in this field and in particular: - the high-activity laboratories at Saclay; - the laboratory for the examination of irradiated fuels at Saclay; - the 'hot' laboratory of the CEN-Grenoble; - the alpha, beta and gamma laboratories of the CEN-Fontenay-aux-Roses. Finally, the report describes the protective materials used for these installations. (author) [French] Les auteurs exposent quels sont les principes generaux qui president a la conception d'un laboratoire a des travaux et des manipulations sur des substances radioactives. Les difficultes a surmonter sont de deux ordres: 1) celles qui proviennent du danger d'irradiation externe; 2) celles qui proviennent du danger de contamination interne. A titre d'exemple, les auteurs decrivent les realisations francaises dans ce domaine et en particulier: - le laboratoire de haute activite de Saclay, - le laboratoire d'examen des combustibles irradies de Saclay, - le laboratoire chaud du CEN-Grenoble, - les laboratoires alpha, beta, gamma du CEN-Fontenay-aux-Roses. Enfin, le rapport decrit les materiaux de protection mis en oeuvre pour ces realisations. (auteur)

  20. 高海拔电气设备特点及设计要求%Electrical Equipment Characteristics and Design Requirements at High Altitude

    Institute of Scientific and Technical Information of China (English)



    高海拔环境对电气设备的要求较高,其绝缘、温升等性能都有特殊性。本文简要分析高海拔环境对电气设备性能的影响,并就其环境下电气设备特点及设计要求提供部分参考。%High altitude environment has higher demand on electrical equipment, and its performances such as insulation properties, temperature rise have particularity. This paper brie-fly analyzes the effect of high altitude environment on electric equipment performance, and provides some reference on el-ectrical equipment characteristics and design requires of its en-vironment.

  1. Facile immobilization of biomolecules onto various surfaces using epoxide-containing antibiofouling polymers. (United States)

    Sung, Daekyung; Park, Sangjin; Jon, Sangyong


    The surface modifications of plastic or glass substrate and the subsequent immobilization of biomolecules onto the surfaces has been a central feature of the fabrication of biochips. To this end, we designed and synthesized new epoxide-containing random copolymers that form stable polymer adlayers on plastic or glass surface and subsequently react with amine or sulfhydryl functional groups of biomolecules under aqueous conditions. Epoxide-containing random copolymers were synthesized by radical polymerization of three functional monomers: a monomer acting as an anchor to the surfaces, a PEG group for preventing nonspecific protein adsorption, and an epoxide group for conjugating to biomolecules. Polymer coating layers were facilely formed on cyclic olefin copolymer (COC) or glass substrate by simply dipping each substrate into a solution of each copolymer. The polymer-coated surfaces characterized by a contact angle analyzer and X-ray photoelectron spectroscopy (XPS) showed very low levels of nonspecific immunoglobulin G (IgG) adsorption compared to the uncoated bare surface (control). Using a microcontact printing (μCP) method, antibodies as representative biomolecules could be selectively attached onto the copolymers-coated glass or COC surface with high signal-to-noise ratios.

  2. Surface enhanced Raman spectroscopy detection of biomolecules using EBL fabricated nanostructured substrates. (United States)

    Peters, Robert F; Gutierrez-Rivera, Luis; Dew, Steven K; Stepanova, Maria


    Fabrication and characterization of conjugate nano-biological systems interfacing metallic nanostructures on solid supports with immobilized biomolecules is reported. The entire sequence of relevant experimental steps is described, involving the fabrication of nanostructured substrates using electron beam lithography, immobilization of biomolecules on the substrates, and their characterization utilizing surface-enhanced Raman spectroscopy (SERS). Three different designs of nano-biological systems are employed, including protein A, glucose binding protein, and a dopamine binding DNA aptamer. In the latter two cases, the binding of respective ligands, D-glucose and dopamine, is also included. The three kinds of biomolecules are immobilized on nanostructured substrates by different methods, and the results of SERS imaging are reported. The capabilities of SERS to detect vibrational modes from surface-immobilized proteins, as well as to capture the protein-ligand and aptamer-ligand binding are demonstrated. The results also illustrate the influence of the surface nanostructure geometry, biomolecules immobilization strategy, Raman activity of the molecules and presence or absence of the ligand binding on the SERS spectra acquired.

  3. Tapered Optical Fiber Sensor for Label-Free Detection of Biomolecules

    Directory of Open Access Journals (Sweden)

    Xingwei Wang


    Full Text Available This paper presents a fast, highly sensitive and low-cost tapered optical fiber biosensor that enables the label-free detection of biomolecules. The sensor takes advantage of the interference effect between the fiber’s first two propagation modes along the taper waist region. The biomolecules bonded on the taper surface were determined by demodulating the transmission spectrum phase shift. Because of the sharp spectrum fringe signals, as well as a relatively long biomolecule testing region, the sensor displayed a fast response and was highly sensitive. To better understand the influence of various biomolecules on the sensor, a numerical simulation that varied biolayer parameters such as thickness and refractive index was performed. The results showed that the spectrum fringe shift was obvious to be measured even when the biolayer was only nanometers thick. A microchannel chip was designed and fabricated for the protection of the sensor and biotesting. Microelectromechanical systems (MEMS fabrication techniques were used to precisely control the profile and depth of the microchannel on the silicon chip with an accuracy of 2 μm. A tapered optical fiber biosensor was fabricated and evaluated with an Immune globulin G (IgG antibody-antigen pair.

  4. Reliable Electronic Equipment

    Directory of Open Access Journals (Sweden)

    N. A. Nayak


    Full Text Available The reliability aspect of electronic equipment's is discussed. To obtain optimum results, close cooperation between the components engineer, the design engineer and the production engineer is suggested.

  5. 舰船电子设备可靠性理论研究%The theoretical research of reliability design for electronic equipment for ships

    Institute of Scientific and Technical Information of China (English)

    李伟; 李燕


    This is the theoretical research of reliability design for electronic equipment for ships. In this paper, through the study of the theory of reliability analysis of ship electronic equipment, study the characteristics of reliability parameters, formulate the reliability allocation and expected scheme and improve ship the inherent reliability of electronic equipment in the development and production stage, so as to reduce ship electronic equipment failure rate, reduce the maintenance support resources, reduce the maintenance cost and prolong the service life of the equipment.%本文是对舰船电子设备可靠性设计的理论研究。本文通过对舰船电子设备进行可靠性理论分析,研究其可靠性参数特点,制定可靠性分配与预计方案,提高舰船电子设备在研制生产阶段的固有可靠性,从而达到降低舰船电子设备故障率、减少维修保障资源、降低维修费用和延长设备使用寿命的目的。

  6. Cooling of electronic equipment

    DEFF Research Database (Denmark)

    A. Kristensen, Anders Schmidt


    Cooling of electronic equipment is studied. The design size of electronic equipment decrease causing the thermal density to increase. This affect the cooling which can cause for example failures of critical components due to overheating or thermal induced stresses. Initially a pin fin heat sink...... is considered as extruded profiles are inadequate for compact designs. An optimal pin fin shape and configuration is sought also taking manufacturing costs into consideration. Standard methods for geometrical modeling and thermal analysis are applied....

  7. Assembly and actuation of nanomaterials using active biomolecules.

    Energy Technology Data Exchange (ETDEWEB)

    Spoerke, Erik David; Thayer, Gayle Echo; de Boer, Maarten Pieter; Bunker, Bruce Conrad; Liu, Jun; Corwin, Alex David; Gaudioso, Jennifer Marie; Sasaki, Darryl Yoshio; Boal, Andrew Kiskadden; Bachand, George David; Trent, Amanda M.; Bachand, Marlene; Rivera, Susan B.; Koch, Steven John


    The formation and functions of living materials and organisms are fundamentally different from those of synthetic materials and devices. Synthetic materials tend to have static structures, and are not capable of adapting to the functional needs of changing environments. In contrast, living systems utilize energy to create, heal, reconfigure, and dismantle materials in a dynamic, non-equilibrium fashion. The overall goal of the project was to organize and reconfigure functional assemblies of nanoparticles using strategies that mimic those found in living systems. Active assembly of nanostructures was studied using active biomolecules to drive the organization and assembly of nanocomposite materials. In this system, kinesin motor proteins and microtubules were used to direct the transport and interactions of nanoparticles at synthetic interfaces. In addition, the kinesin/microtubule transport system was used to actively assemble nanocomposite materials capable of storing significant elastic energy. Novel biophysical measurement tools were also developed for measuring the collective force generated by kinesin motor proteins, which will provide insight on the mechanical constraints of active assembly processes. Responsive reconfiguration of nanostructures was studied in terms of using active biomolecules to mediate the optical properties of quantum dot (QD) arrays through modulation of inter-particle spacing and associated energy transfer interaction. Design rules for kinesin-based transport of a wide range of nanoscale cargo (e.g., nanocrystal quantum dots, micron-sized polymer spheres) were developed. Three-dimensional microtubule organizing centers were assembled in which the polar orientation of the microtubules was controlled by a multi-staged assembly process. Overall, a number of enabling technologies were developed over the course of this project, and will drive the exploitation of energy-driven processes to regulate the assembly, disassembly, and dynamic

  8. 浅析哈萨克斯坦石油化工装置消防设计%Analysis of fire protection design of petrochemical equipments in Kazakhstan

    Institute of Scientific and Technical Information of China (English)



    There are lots of differences between the fire protection requirements of petrochemical equip-ments in China and Kazakhstan which mentioned in the codes and routines for fire protection design. The code for fire protection design of petrochemical equipments in Kazakhstan was introduced; besides, the differences be-tween the codes and routines for fire protection of petrochemical equipments in Kazakhstan and China were ana-lyzed and compared from aspects of fire-fighting cooling water system; foam extinguishing system, steam-smoth-ering system;dry powder extinguishing system;automatic gas extinguishing system and fire demand determination.%在石油化工装置的消防设计上,哈萨克斯坦与国内在设计规范及设计惯例等方面存在诸多差异。介绍了哈萨克斯坦石油化工装置消防规范,并从消防冷却水系统、泡沫灭火系统、蒸汽灭火系统、干粉灭火系统、自动气体灭火系统及消防用水量确定等方面,分析比较了哈萨克斯坦与国内石油化工装置消防设计规范及设计惯例的不同之处。

  9. Design and Realization of Integrated Management System for Data Interoperability between Point-of-Care Testing Equipment and Hospital Information System (United States)

    Park, Ki Sang; Heo, Hyuk


    Objectives The purpose of this study was to design an integrated data management system based on the POCT1-A2, LIS2-A, LIS2-A2, and HL7 standard to ensure data interoperability between mobile equipment, such as point-of-care testing equipment and the existing hospital data system, its efficiency was also evaluated. Methods The method of this study was intended to design and realize a data management system which would provide a solution for the problems that occur when point-of-care testing equipment is introduced to existing hospital data, after classifying such problems into connectivity, integration, and interoperability. This study also checked if the data management system plays a sufficient role as a bridge between the point-of-care testing equipment and the hospital information system through connection persistence and reliability testing, as well as data integration and interoperability testing. Results In comparison with the existing system, the data management system facilitated integration by improving the result receiving time, improving the collection rate, and by enabling the integration of disparate types of data into a single system. And it was found out that we can solve the problems related to connectivity, integration and interoperability through generating the message in standardized types. Conclusions It is expected that the proposed data management system, which is designed to improve the integration point-of-care testing equipment with existing systems, will establish a solid foundation on which better medical service may be provided by hospitals by improving the quality of patient service. PMID:24175121

  10. Reaction between protein radicals and other biomolecules

    DEFF Research Database (Denmark)

    Østdal, Henrik; Davies, Michael Jonathan; Andersen, Henrik J


    oil-in-water emulsion resulted in additional formation of lipid hydroperoxides and conjugated dienes. The results clearly show that protein radicals have to be considered as dynamic species during oxidative processes in biological systems and that protein radicals should not be considered as end-products......The present study investigates the reactivity of bovine serum albumin (BSA) radicals towards different biomolecules (urate, linoleic acid, and a polypeptide, poly(Glu-Ala-Tyr)). The BSA radical was formed at room temperature through a direct protein-to-protein radical transfer from H(2)O(2....... Subsequent analysis showed a decrease in the concentration of urate upon reaction with the BSA radical, while the BSA radical in the presence of poly(Glu-Ala-Tyr) resulted in increased formation of the characteristic protein oxidation product, dityrosine. Reaction between the BSA radical and a linoleic acid...

  11. Generalized design of fitness equipment control interface%健身器材控制界面的通用化设计

    Institute of Scientific and Technical Information of China (English)

    林能涛; 苏智剑; 乔帅; 闫扬义; 闫勇杰


    BACKGROUND:Health has been paid more and more attention in modern society, the health of those special groups, such as the elderly and the disabled, should also be brought to the forefront. As fitness equipment is more and more popular in contemporary, our focus should be transferred to the use of fitness equipment for these special groups. OBJECTIVE:To put forward the design strategy of fitness equipment control interface, for the special groups to use common fitness equipment comfortably and conveniently. METHODS:Based on the research of existing fitness equipment control interface, according to the usage habits and cognitive awareness of special groups on control interface, a new design strategy has been put forward using the general design method and ergonomics. RESULTS AND CONCLUSION:Based on the existing 45 kinds of exercise bikes, 84 kinds of running machines and 129 kinds of treadmil fitness equipments, we found that these equipments are designed for common users in the aspects of design and production process and control interface. Universal design is a design concept beyond the lack of capacity to meet the different needs of different populations, and designed products can reduce or even eliminate the difference in the ability of special populations and the general population in use. The new strategy makes design of fitness equipment control interface generalized and good man-machine interactive, which can meet the demand of the special groups and help them do fitness exercise to improve their health and body function.%背景:随着健身器材越来越普及的当代国情,关注的焦点也应该转移到老年人、残疾人等这些特殊群体对健身器材的使用上。  目的:提出健身器材控制界面通用化设计策略,以便于特殊人群能够舒适方便的使用普通健身器材。  方法:通过对现有健身器材控制界面的调查研究,结合特殊人群对控制界面的使用习惯和认知意识,

  12. Drilling equipment

    Energy Technology Data Exchange (ETDEWEB)

    Voyevodin, Yu.M.; Kholosha, Ye.G.; Leshchenko, A.G.; Timchenko, A.I.


    The invention refers to units designed for extracting minerals by mechanical action on the face by a cutting tool. There is a known drills which includes a pulse device which contains a combustion chamber, cylinder, piston-percussion tool and cutting tool. The combustion chamber and the sub-piston cavity of the cylinder are connected by a channel in which there is a distributor. Its shortcoming is the fact that the pulse device does not guarantee the necessary motion of the actuating mechanism for its velocity and frequency of the shocks on the cutting tool. This reduces the efficiency of the operation and limits the area of application. The purpose of the proposed invention is to improve productivity of the device. This is achieved because the head of the drill is equipped with a 2-piston pulse device which contains 2-combustion chambers, 2 working cylinders, 2 piston percussion instruments, a rod with gas-conducting and connecting channels for interconnection of the sub-piston cavities and combustion chambers of both cylinders. The spent gases of one cylinder are used for closing the combustion chamber of the second cylinder.

  13. High-yielding and photolabile approaches to the covalent attachment of biomolecules to surfaces via hydrazone chemistry. (United States)

    Lee, Ju Hun; Domaille, Dylan W; Noh, Hyunwoo; Oh, Taeseok; Choi, Chulmin; Jin, Sungho; Cha, Jennifer N


    The development of strategies to couple biomolecules covalently to surfaces is necessary for constructing sensing arrays for biological and biomedical applications. One attractive conjugation reaction is hydrazone formation--the reaction of a hydrazine with an aldehyde or ketone--as both hydrazines and aldehydes/ketones are largely bioorthogonal, which makes this particular reaction suitable for conjugating biomolecules to a variety of substrates. We show that the mild reaction conditions afforded by hydrazone conjugation enable the conjugation of DNA and proteins to the substrate surface in significantly higher yields than can be achieved with traditional bioconjugation techniques, such as maleimide chemistry. Next, we designed and synthesized a photocaged aryl ketone that can be conjugated to a surface and photochemically activated to provide a suitable partner for subsequent hydrazone formation between the surface-anchored ketone and DNA- or protein-hydrazines. Finally, we exploit the latent functionality of the photocaged ketone and pattern multiple biomolecules on the same substrate, effectively demonstrating a strategy for designing substrates with well-defined domains of different biomolecules. We expect that this approach can be extended to the production of multiplexed assays by using an appropriate mask with sequential photoexposure and biomolecule conjugation steps.

  14. Preparation of supramolecular hydrogel-enzyme hybrids exhibiting biomolecule-responsive gel degradation. (United States)

    Shigemitsu, Hajime; Fujisaku, Takahiro; Onogi, Shoji; Yoshii, Tatsuyuki; Ikeda, Masato; Hamachi, Itaru


    Hydrogelators are small, self-assembling molecules that form supramolecular nanofiber networks that exhibit unique dynamic properties. Development of supramolecular hydrogels that degrade in response to various biomolecules could potentially be used for applications in areas such as drug delivery and diagnostics. Here we provide a synthetic procedure for preparing redox-responsive supramolecular hydrogelators that are used to create hydrogels that degrade in response to oxidizing or reducing conditions. The synthesis takes ∼2-4 d, and it can potentially be carried out in parallel to prepare multiple hydrogelator candidates. This described solid-phase peptide synthesis protocol can be used to produce previously described hydrogelators or to construct a focused molecular library to efficiently discover and optimize new hydrogelators. In addition, we describe the preparation of redox-responsive supramolecular hydrogel-enzyme hybrids that are created by mixing aqueous solutions of hydrogelators and enzymes, which requires 2 h for completion. The resultant supramolecular hydrogel-enzyme hybrids exhibit gel degradation in response to various biomolecules, and can be rationally designed by connecting the chemical reactions of the hydrogelators with enzymatic reactions. Gel degradation in response to biomolecules as triggers occurs within a few hours. We also describe the preparation of hydrogel-enzyme hybrids arrayed on flat glass slides, enabling high-throughput analysis of biomolecules such as glucose, uric acid, lactate and so on by gel degradation, which is detectable by the naked eye. The protocol requires ∼6 h to prepare the hydrogel-enzyme hybrid array and to complete the biomolecule assay.

  15. Heme biomolecule as redox mediator and oxygen shuttle for efficient charging of lithium-oxygen batteries (United States)

    Ryu, Won-Hee; Gittleson, Forrest S.; Thomsen, Julianne M.; Li, Jinyang; Schwab, Mark J.; Brudvig, Gary W.; Taylor, André D.


    One of the greatest challenges with lithium-oxygen batteries involves identifying catalysts that facilitate the growth and evolution of cathode species on an oxygen electrode. Heterogeneous solid catalysts cannot adequately address the problematic overpotentials when the surfaces become passivated. However, there exists a class of biomolecules which have been designed by nature to guide complex solution-based oxygen chemistries. Here, we show that the heme molecule, a common porphyrin cofactor in blood, can function as a soluble redox catalyst and oxygen shuttle for efficient oxygen evolution in non-aqueous Li-O2 batteries. The heme's oxygen binding capability facilitates battery recharge by accepting and releasing dissociated oxygen species while benefiting charge transfer with the cathode. We reveal the chemical change of heme redox molecules where synergy exists with the electrolyte species. This study brings focus to the rational design of solution-based catalysts and suggests a sustainable cross-link between biomolecules and advanced energy storage.

  16. Design and Installation of Lecture Hall Lighting and Acoustic Equipment%报告厅灯光音响设备的设计与安装

    Institute of Scientific and Technical Information of China (English)



    专业灯光音响工程是一项对技术要求较高的综合性工程,其涉及到建筑、电工、电子、声学、光学等专业性知识,在具体的工程实施过程中,又分为场地考察、分析计算、设计选型、施工调试等几个阶段。文中在讨论灯光系统的设计与调整、音响设备,并时设备的安装进行研究分析,以给学术报告厅灯光音响设备的设计与安装提供一定的参考。%Professional lighting and acoustic engineering is a comprehensive project which has a high technical standard. It involves construction, electrical engineering, electronics, acoustics, optics and other professional fields. In a specific project implementation process, it is divided into such stages as site visits, analysis of calcula- tion, design selection, construction and commissioning. This article aims to discuss the design and adjustment of the lighting system and acoustic equipment and analyze the installation of equipment. It is of practical value for the design and installation of lighting and acoustic equipment for the lecture.

  17. GroPBS: Fast Solver for Implicit Electrostatics of Biomolecules

    Directory of Open Access Journals (Sweden)

    Franziska eBertelshofer


    Full Text Available Knowledge about the electrostatic potential on the surface of biomolecules or biomembranes under physiological conditions is an important step in the attempt to characterize the physico-chemical properties of these molecules and in particular also their interactions with each other. Additionally, knowledge about solution electrostatics may guide also the design of molecules with specified properties. However, explicit water models come at a high computational cost, rendering them unsuitable for large design studies or for docking purposes. Implicit models with the water phase treated as a continuum require the numerical solution of the Poisson-Boltzmann Equation (PBE. Here, we present a new flexible program for the numerical solution of the PBE, allowing for different geometries, and the explicit and implicit inclusion of membranes. It involves a discretization of space and the computation of the molecular surface. The PBE is solved using finite differences, the resulting set of equations is solved using a Gauss-Seidel method. It is shown for the example of the sucrose transporter ScrY that the implicit inclusion of a surrounding membrane has a strong effect also on the electrostatics within the pore region and thus need to be carefully considered e.g. in design studies on membrane proteins.

  18. Ionic strength independence of charge distributions in solvation of biomolecules

    Energy Technology Data Exchange (ETDEWEB)

    Virtanen, J. J. [Department of Chemistry, University of Chicago, Chicago, Illinois 60637 (United States); James Franck Institute, University of Chicago, Chicago, Illinois 60637 (United States); Sosnick, T. R. [Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, University of Chicago, Chicago, Illinois 60637 (United States); Computation Institute, University of Chicago, Chicago, Illinois 60637 (United States); Freed, K. F. [Department of Chemistry, University of Chicago, Chicago, Illinois 60637 (United States); James Franck Institute, University of Chicago, Chicago, Illinois 60637 (United States); Computation Institute, University of Chicago, Chicago, Illinois 60637 (United States)


    Electrostatic forces enormously impact the structure, interactions, and function of biomolecules. We perform all-atom molecular dynamics simulations for 5 proteins and 5 RNAs to determine the dependence on ionic strength of the ion and water charge distributions surrounding the biomolecules, as well as the contributions of ions to the electrostatic free energy of interaction between the biomolecule and the surrounding salt solution (for a total of 40 different biomolecule/solvent combinations). Although water provides the dominant contribution to the charge density distribution and to the electrostatic potential even in 1M NaCl solutions, the contributions of water molecules and of ions to the total electrostatic interaction free energy with the solvated biomolecule are comparable. The electrostatic biomolecule/solvent interaction energies and the total charge distribution exhibit a remarkable insensitivity to salt concentrations over a huge range of salt concentrations (20 mM to 1M NaCl). The electrostatic potentials near the biomolecule's surface obtained from the MD simulations differ markedly, as expected, from the potentials predicted by continuum dielectric models, even though the total electrostatic interaction free energies are within 11% of each other.

  19. Design of Power Supply Monitoring System for Fire Protection Equipment%消防设备电源监控系统设计

    Institute of Scientific and Technical Information of China (English)



    对消防设备电源监控系统相关规范及标准进行分析及梳理,分析归纳消防设备电源、消防设备电源监控系统的定义及作用,结合某大型城市综合体工程,介绍消防设备电源监控系统的设置场所、范围、部位、布线等设计。%Relevant national regulations and standards regarding power supply monitoring system for fire protection equipment are analyzed and summarized. Definitions and functions of power supply for fire protection equipments and power supply monitoring systems for fire protection equipment are analyzed and summarized. Based on a large urban complex project, the setting place, range, location, wiring and other designs of power supply monitoring system for fire protection equipment are introduced.

  20. Noncovalent Labeling of Biomolecules with Red and Near- Infrared Dyes

    Directory of Open Access Journals (Sweden)

    Lucjan Strekowski


    Full Text Available Biopolymers such as proteins and nucleic acids can be labeled with a fluorescent marker to allow for their detection. Covalent labeling is achieved by the reaction of an appropriately functionalized dye marker with a reactive group on a biomolecule. The recent trend, however, is the use of noncovalent labeling that results from strong hydrophobic and/or ionic interactions between the marker and biomolecule of interest. The main advantage of noncovalent labeling is that it affects the functional activity of the biomolecule to a lesser extent. The applications of luminescent cyanine and squarylium dyes are reviewed.

  1. Biomolecule-mediated synthesis of nanocrystalline semiconductors (United States)

    Bae, Weon

    CdS and ZnS nanocrystalline semiconductors (NCs) were prepared by titrating inorganic sulfide into preformed Cd(II)- or Zn(II)-complexes of phytochelatins, glutathione or cysteine. This strategy resulted in the formation NCs capped by the chosen biomolecule. The range of sizes and their distributions depended primarily on the quantity of sulfide titrated and the biomolecule chosen for the initial metallo-complex. The processes of NC formation were studied by absorption and fluorescence spectrophotometry. The size distribution was analyzed by gel permeation chromatography. Ethanol precipitation of NCs under aqueous conditions was used to isolate nanoparticles within a very narrow size-range. Reduction of selected dyes was also studied on the surfaces of NCs. Glutathione-capped CdS nanoparticles exhibited significant size heterogeneity even at a single sulfide titration. In contrast, phytochelatins showed much less dispersion in size at a given sulfide titration. Phytochelatins could replace glutathione without changing the size of glutathione-capped CdS nanoparticles. Cysteine appeared to be intermediate between glutathione and phytochelatins in the formation of CdS nanoparticles. The calculated radii, using an effective mass approximation method, were 10.8-17.3, 10.6-11.8, and 13.5-15.5A for glutathione-, phytochelatin-, and cysteine-capped CdS nanoparticles, respectively. Cysteine-capped ZnS showed narrower size distribution than glutathione-capped ZnS. However, elevated temperatures were necessary to accomplish optimal yields of cysteine-capped ZnS NCs. An additional control over the size distribution of NCs was achieved by size-selective precipitation with ethanol. These procedures led to the isolation of nanoparticles that were more uniform in size and chemical compositions as determined by spectroscopic and chemical analyses of size-fractionated samples. Precipitation also allowed preparation of large quantities of powdered nanoparticles that could be

  2. 水力空化水处理设备的控制系统设计%The Design of Control System for Hydrodynmic Cavitation Water Treatment Equipment

    Institute of Scientific and Technical Information of China (English)

    刘志猛; 陈平; 邓橙; 苑英海; 朱孟府


    为了完成对水力空化水处理设备的自动控制,结合水力空化水处理技术工艺,应用PLC控制器和触摸屏技术,开发并设计一套水力空化水处理设备的控制系统.采用触摸屏作为人机界面,可实现pH、ORP、电导率、DO等水质参数实时检测、设定和修改等功能,并能方便直观地对水力空化水处理设备的工作过程进行监控.经过实际运行和调试,基于PLC和触摸屏的水力空化水处理设备的控制系统稳定性好、可靠性高、易于维护,提高了水力空化水处理设备的效率和自动化水平.%In order to complete automatic control of the hydrodynamic cavitation water treatment equipment, by adopting PLC and touch screen technologies, a control system of the hydrodynamic cavitation water treatment equipment is designed and developed it is based on technological process of the hydrodynamic cavitation water treatment. The system equips with touch screen as the human machine interface implementes the functions of real-time detection, parameter setting and modification of the water quality parameters such as pH, ORP, TDS and DO. Thus the operating process of the hydrodynamic cavitation water treatment equipment can be easily and directly perceived and controlled. The control system of the hydrodynamic cavitation water treatment equipment has good stability and reliability, and is easy to maintenance for enhancing operation efficiency and automation level after practically working and debugging.

  3. 基于DRFM技术的雷达标校器设计与实现%Design and Accomplishment of Radar Calibration Equipment Based on DRFM

    Institute of Scientific and Technical Information of China (English)

    刘勇; 李国君


    建立了基于数字射频存储器技术构建雷达标校器的方法.该雷达标校器可灵活实现目标运动模拟、多普勒频率加载、目标RCS模拟、多目标模拟等功能,较传统的基于光纤延迟实现的雷达标校器更具有应用价值.针对实际应用需求提出了解决频率捷变的方案,给出了典型模拟策略所需数学模型.应用研究表明,基于数字射频存储器技术的雷达标校器具有较强的工程应用价值和推广价值.%A design making of radar calibration equipment based on digital radio frequency memory (DRFM)technique was presented. The equipment can flexibly accomplish target motion simulation, Doppler frequency loading, target RCS simulation functions and so on. This radar calibration equipment is more practical comparing the calibration equipments using fibre delay. A programmed for settling frequency agility was put forwarded for practical demand. At the same time a mathematics model of typical simulating tactics was brought out. The research results showed that this radar calibration equipment base on digital radio frequency memory (DRFM) had much engineering and realizing values.

  4. Interfacial water thickness at inorganic nanoconstructs and biomolecules: Size matters

    Energy Technology Data Exchange (ETDEWEB)

    Cardellini, Annalisa; Fasano, Matteo; Chiavazzo, Eliodoro; Asinari, Pietro, E-mail:


    Water molecules in the proximity of solid nanostructures influence both the overall properties of liquid and the structure and functionality of solid particles. The study of water dynamics at solid–liquid interfaces has strong implications in energy, environmental and biomedical fields. This article focuses on the hydration layer properties in the proximity of Carbon Nanotubes (CNTs) and biomolecules (proteins, polypeptides and amino acids). Here we show a quantitative relation between the solid surface extension and the characteristic length of water nanolayer (δ), which is confined at solid–liquid interfaces. Specifically, the size dependence is attributed to the limited superposition of nonbonded interactions in case of small molecules. These results may facilitate the design of novel energy or biomedical colloidal nanosuspensions, and a more fundamental understanding of biomolecular processes influenced by nanoscale water dynamics. - Highlights: • Properties of the water hydration layer are investigated. • New relation between extension of solid size and hydration layer established. • Possible impact on rational design of nanosuspensions.

  5. Engineered Carbon-Nanomaterial-Based Electrochemical Sensors for Biomolecules. (United States)

    Tiwari, Jitendra N; Vij, Varun; Kemp, K Christian; Kim, Kwang S


    The study of electrochemical behavior of bioactive molecules has become one of the most rapidly developing scientific fields. Biotechnology and biomedical engineering fields have a vested interest in constructing more precise and accurate voltammetric/amperometric biosensors. One rapidly growing area of biosensor design involves incorporation of carbon-based nanomaterials in working electrodes, such as one-dimensional carbon nanotubes, two-dimensional graphene, and graphene oxide. In this review article, we give a brief overview describing the voltammetric techniques and how these techniques are applied in biosensing, as well as the details surrounding important biosensing concepts of sensitivity and limits of detection. Building on these important concepts, we show how the sensitivity and limit of detection can be tuned by including carbon-based nanomaterials in the fabrication of biosensors. The sensing of biomolecules including glucose, dopamine, proteins, enzymes, uric acid, DNA, RNA, and H2O2 traditionally employs enzymes in detection; however, these enzymes denature easily, and as such, enzymeless methods are highly desired. Here we draw an important distinction between enzymeless and enzyme-containing carbon-nanomaterial-based biosensors. The review ends with an outlook of future concepts that can be employed in biosensor fabrication, as well as limitations of already proposed materials and how such sensing can be enhanced. As such, this review can act as a roadmap to guide researchers toward concepts that can be employed in the design of next generation biosensors, while also highlighting the current advancements in the field.

  6. Nanobiodevices for Biomolecule Analysis and Imaging (United States)

    Yasui, Takao; Kaji, Noritada; Baba, Yoshinobu


    Nanobiodevices have been developed to analyze biomolecules and cells for biomedical applications. In this review, we discuss several nanobiodevices used for disease-diagnostic devices, molecular imaging devices, regenerative medicine, and drug-delivery systems and describe the numerous advantages of nanobiodevices, especially in biological, medical, and clinical applications. This review also outlines the fabrication technologies for nanostructures and nanomaterials, including top-down nanofabrication and bottom-up molecular self-assembly approaches. We describe nanopillar arrays and nanowall arrays for the ultrafast separation of DNA or protein molecules and nanoball materials for the fast separation of a wide range of DNA molecules, and we present examples of applications of functionalized carbon nanotubes to obtain information about subcellular localization on the basis of mobility differences between free fluorophores and fluorophore-labeled carbon nanotubes. Finally, we discuss applications of newly synthesized quantum dots to the screening of small interfering RNA, highly sensitive detection of disease-related proteins, and development of cancer therapeutics and diagnostics.

  7. Microfluidic Radiometal Labeling Systems for Biomolecules

    Energy Technology Data Exchange (ETDEWEB)

    Reichert, D E; Kenis, P J. A.


    In a typical labeling procedure with radiometals, such as Cu-64 and Ga-68; a very large (~ 100-fold) excess of the non-radioactive reactant (precursor) is used to promote rapid and efficient incorporation of the radioisotope into the PET imaging agent. In order to achieve high specific activities, careful control of reaction conditions and extensive chromatographic purifications are required in order to separate the labeled compounds from the cold precursors. Here we propose a microfluidic approach to overcome these problems, and achieve high specific activities in a more convenient, semi-automated fashion and faster time frame. Microfluidic reactors, consisting of a network of micron-sized channels (typical dimensions in the range 10 - 300¼m), filters, separation columns, electrodes and reaction loops/chambers etched onto a solid substrate, are now emerging as an extremely useful technology for the intensification and miniaturization of chemical processes. The ability to manipulate, process and analyze reagent concentrations and reaction interfaces in both space and time within the channel network of a microreactor provides the fine level of reaction control that is desirable in PET radiochemistry practice. These factors can bring radiometal labeling, specifically the preparation of radio-labeled biomolecules such as antibodies, much closer to their theoretical maximum specific activities.

  8. Formation of biomolecule precursors in space

    Energy Technology Data Exchange (ETDEWEB)

    Geppert, W D [Department of Physics, Stockholm University, Alba Nova, Roslagstullbacken 21, SE-10691, Stockholm (Sweden); Vigren, E [Department of Physics, Stockholm University, Alba Nova, Roslagstullbacken 21, SE-10691, Stockholm (Sweden); Hamberg, M [Department of Physics, Stockholm University, Alba Nova, Roslagstullbacken 21, SE-10691, Stockholm (Sweden); Zhaunerchyk, V [Department of Physics, Stockholm University, Alba Nova, Roslagstullbacken 21, SE-10691, Stockholm (Sweden); Thomas, R D [Department of Physics, Stockholm University, Alba Nova, Roslagstullbacken 21, SE-10691, Stockholm (Sweden); Kaminska, M [Institute of Physics, Swieetokrzyska Academy, ul. Swieokrzyska 15, PL-25406, Kielce (Poland); Millar, T J [Astrophysics Research Centre, Queen' s University Belfast, BT7 1NN, Belfast, Northern Ireland (United Kingdom); Semaniak, J [Institute of Physics, Swieetokrzyska Academy, ul. Swieokrzyska 15, PL-25406, Kielce (Poland); Roberts, H [Astrophysics Research Centre, Queen' s University Belfast, BT7 1NN, Belfast, Northern Ireland (United Kingdom); Hellberg, F [Department of Physics, Stockholm University, Alba Nova, Roslagstullbacken 21, SE-10691, Stockholm (Sweden); Oesterdahl, F [Department of Physics, Royal Institute of Technology, Alba Nova, Roslagstullbacken 21, SE-10691, Stockholm (Sweden); Ehlerding, A [Department of Physics, Stockholm University, Alba Nova, Roslagstullbacken 21, SE-10691, Stockholm (Sweden); Larsson, M [Department of Physics, Stockholm University, Alba Nova, Roslagstullbacken 21, SE-10691, Stockholm (Sweden)


    Alcohols and nitriles not only play an important role as templates for synthesis of larger molecules in the interstellar medium and planetary atmospheres, but they can also be regarded as precursors for biomolecules. Alcohols can form carbohydrates through reaction with HCO and nitriles can be hydrolysed to amino acids in aqueous solutions, which is the final step of the well-known Strecker synthesis. Therefore the question of the pathways of formation of alcohols and nitriles and the efficiency and the product distribution of their subsequent degradation reactions in the above-mentioned astrophysical environments is of great interest. In both processes dissociative recombination reactions of protonated nitriles and alcohols may play a major role and are included in models of interstellar clouds and planetary atmospheres. However, the reaction rate coefficients and product branching ratios for the majority of these processes are so far still unknown, which adversely affects the quality of predictions of model calculations. In this Contribution, we therefore present branching ratios and rate constants of the dissociative recombination of protonated methanol (CH{sub 3}OH{sub 2}), as well as protonated acetonitrile (CH{sub 3}CNH{sup +}), acrylonitrile (C{sub 2}H{sub 3}CNH{sup +}) and cyanoacetylene (HC{sup 3}NH{sup +}). The impact of the obtained new data on model calculations of abundances of important interstellar molecules in dark clouds is discussed.

  9. Work Breakdown Structure and Plant/Equipment Designation System Numbering Scheme for the High Temperature Gas- Cooled Reactor (HTGR) Component Test Capability (CTC)

    Energy Technology Data Exchange (ETDEWEB)

    Jeffrey D Bryan


    This white paper investigates the potential integration of the CTC work breakdown structure numbering scheme with a plant/equipment numbering system (PNS), or alternatively referred to in industry as a reference designation system (RDS). Ideally, the goal of such integration would be a single, common referencing system for the life cycle of the CTC that supports all the various processes (e.g., information, execution, and control) that necessitate plant and equipment numbers be assigned. This white paper focuses on discovering the full scope of Idaho National Laboratory (INL) processes to which this goal might be applied as well as the factors likely to affect decisions about implementation. Later, a procedure for assigning these numbers will be developed using this white paper as a starting point and that reflects the resolved scope and outcome of associated decisions.

  10. 移动式放射性废液接收处理装置设计%Design of Mobile Receiving and Treatment Equipment for Radioactive Liquid Waste

    Institute of Scientific and Technical Information of China (English)

    孔劲松; 吕景彬; 郭卫群


    The advantage and disadvantage of radioactive liquid waste treatment technology are analyzed in this paper. The experimental disposal equipment for radioactive liquid waste with complicated sources is designed by combining the far infrared calefaction technology with evaporation technology. It has advantages of low energy consuming and high decontamination efficiency. The frothy and dirt appear rarely in this equipment.%分析多种放射性废液处理方法的优缺点,结合远红外辐射加热技术与蒸发处理技术,设计一套适合复杂源项废液的移动式接收处理的试验装置,该装置具有能耗低、去污效率高、不易起泡、不易结垢等特点.

  11. On the mobility of biomolecules : a fluorescence microscopy approach

    NARCIS (Netherlands)

    Bogaart, Geert van den


    This thesis describes the development and application of a number of fluorescence spectroscopy related techniques (FCS, FRAP, DCFBA) to measure diffusion of biomolecules in cells, in membranes and through membrane pores.

  12. Cellular content of biomolecules in sub-seafloor microbial communities

    DEFF Research Database (Denmark)

    Braun, Stefan; Morono, Yuki; Becker, Kevin W.;


    Microbial biomolecules, typically from the cell envelope, can provide crucial information about distribution, activity, and adaptations of sub-seafloor microbial communities. However, when cells die these molecules can be preserved in the sediment on timescales that are likely longer than...... the lifetime of their microbial sources. Here we provide for the first time measurements of the cellular content of biomolecules in sedimentary microbial cells. We separated intact cells from sediment matrices in samples from surficial, deeply buried, organic-rich, and organic-lean marine sediments by density...... and mass spectrometry for biomolecule analyses. Because cell extracts from density centrifugation still contained considerable amounts of detrital particles and non-cellular biomolecules, we further purified cells from two samples by fluorescence-activated cell sorting (FACS). Cells from these highly...

  13. Fullerene-biomolecule conjugates and their biomedicinal applications. (United States)

    Yang, Xinlin; Ebrahimi, Ali; Li, Jie; Cui, Quanjun


    Fullerenes are among the strongest antioxidants and are characterized as "radical sponges." The research on biomedicinal applications of fullerenes has achieved significant progress since the landmark publication by Friedman et al in 1993. Fullerene-biomolecule conjugates have become an important area of research during the past 2 decades. By a thorough literature search, we attempt to update the information about the synthesis of different types of fullerene-biomolecule conjugates, including fullerene-containing amino acids and peptides, oligonucleotides, sugars, and esters. Moreover, we also discuss in this review recently reported data on the biological and pharmaceutical utilities of these compounds and some other fullerene derivatives of biomedical importance. While within the fullerene-biomolecule conjugates, in which fullerene may act as both an antioxidant and a carrier, specific targeting biomolecules conjugated to fullerene will undoubtedly strengthen the delivery of functional fullerenes to sites of clinical interest.

  14. Surface manipulation of biomolecules for cell microarray applications. (United States)

    Hook, Andrew L; Thissen, Helmut; Voelcker, Nicolas H


    Many biological events, such as cellular communication, antigen recognition, tissue repair and DNA linear transfer, are intimately associated with biomolecule interactions at the solid-liquid interface. To facilitate the study and use of these biological events for biodevice and biomaterial applications, a sound understanding of how biomolecules behave at interfaces and a concomitant ability to manipulate biomolecules spatially and temporally at surfaces is required. This is particularly true for cell microarray applications, where a range of biological processes must be duly controlled to maximize the efficiency and throughput of these devices. Of particular interest are transfected-cell microarrays (TCMs), which significantly widen the scope of microarray genomic analysis by enabling the high-throughput analysis of gene function within living cells. This article reviews this current research focus, discussing fundamental and applied research into the spatial and temporal surface manipulation of DNA, proteins and other biomolecules and the implications of this work for TCMs.

  15. Probing nanoparticles and nanoparticle-conjugated biomolecules using time-of-flight secondary ion mass spectrometry. (United States)

    Kim, Young-Pil; Shon, Hyun Kyong; Shin, Seung Koo; Lee, Tae Geol


    Bio-conjugated nanoparticles have emerged as novel molecular probes in nano-biotechnology and nanomedicine and chemical analyses of their surfaces have become challenges. The time-of-flight (TOF) secondary ion mass spectrometry (SIMS) has been one of the most powerful surface characterization techniques for both nanoparticles and biomolecules. When combined with various nanoparticle-based signal enhancing strategies, TOF-SIMS can probe the functionalization of nanoparticles as well as their locations and interactions in biological systems. Especially, nanoparticle-based SIMS is an attractive approach for label-free drug screening because signal-enhancing nanoparticles can be designed to directly measure the enzyme activity. The chemical-specific imaging analysis using SIMS is also well suited to screen nanoparticles and nanoparticle-biomolecule conjugates in complex environments. This review presents some recent applications of nanoparticle-based TOF-SIMS to the chemical analysis of complex biological systems.

  16. Fed-Batch Biomolecule Production by Bacillus subtilis: A State of the Art Review. (United States)

    Öztürk, Sibel; Çalık, Pınar; Özdamar, Tunçer H


    Bacillus subtilis is a highly promising production system for various biomolecules. This review begins with the algorithm of fed-batch operations (FBOs) and then illustrates the approaches to design the initial production medium and/or feed stream. Additionally, the feeding strategies developed with or without feedback control for fed-batch B. subtilis fermentations were compiled with a special emphasis on recombinant protein (r-protein) production. For biomolecule production by wild-type B. subtilis, due to the different intracellular production patterns, no consensus exists on the FBO strategy that gives the maximum productivity, whereas for r-protein production appropriate feeding strategies vary depending on the promoter used. Thus, we conclude that the B. subtilis community is still seeking an approved strong promoter and generalized FBO strategies.

  17. Design of thermal reliability platform for electronic equipment%电子设备的热可靠性平台设计

    Institute of Scientific and Technical Information of China (English)



    本文是针对于电子设备的热属性测试和热设计优化建立一个可靠性应用研发平台。平台分为热测试和热设计两个模块,热测试半导体器件的热学属性的精确测量和外围散热设备的选型和验证,并结合热设计部分,保证仿真结果的准确性,并可进行结构和选型的优化,平台囊括电力电子产品化过程中热可靠性设计的所有环节。%This paper is aimed at the thermal property testing and thermal design optimization of electronic equipment to establish a reliable application research and development platform.Selection and validation of the platform is divided into two modules,thermal test and thermal design,thermal test semiconductor device thermal properties of accurate measurement and peripheral cooling equipment,combined with thermal design,ensure the accuracy of the simulation results,and can carry on the optimization of structure and selection,the platform include thermal reliability design in the course of power electronic products in all aspects.

  18. Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: A review

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Cheng; Denno, Madelaine E.; Pyakurel, Poojan; Venton, B. Jill, E-mail:


    Carbon nanomaterials are advantageous for electrochemical sensors because they increase the electroactive surface area, enhance electron transfer, and promote adsorption of molecules. Carbon nanotubes (CNTs) have been incorporated into electrochemical sensors for biomolecules and strategies have included the traditional dip coating and drop casting methods, direct growth of CNTs on electrodes and the use of CNT fibers and yarns made exclusively of CNTs. Recent research has also focused on utilizing many new types of carbon nanomaterials beyond CNTs. Forms of graphene are now increasingly popular for sensors including reduced graphene oxide, carbon nanohorns, graphene nanofoams, graphene nanorods, and graphene nanoflowers. In this review, we compare different carbon nanomaterial strategies for creating electrochemical sensors for biomolecules. Analytes covered include neurotransmitters and neurochemicals, such as dopamine, ascorbic acid, and serotonin; hydrogen peroxide; proteins, such as biomarkers; and DNA. The review also addresses enzyme-based electrodes that are used to detect non-electroactive species such as glucose, alcohols, and proteins. Finally, we analyze some of the future directions for the field, pointing out gaps in fundamental understanding of electron transfer to carbon nanomaterials and the need for more practical implementation of sensors. - Highlights: • We review the types of carbon nanomaterials used in electrochemical sensors. • Different materials and sensor designs are compared for classes of biomolecules. • Future challenges of better sensor design and implementation are assessed.

  19. Biomolecule conjugation strategy using novel water-soluble phosphine-based chelating agents (United States)

    Katti, Kattesh V.; Gali, Hariprasad; Volkert, Wynn A.


    This invention describes a novel strategy to produce phosphine-functionalized biomolecules (e.g. peptides or proteins) for potential use in the design and development of site-specific radiopharmaceuticals for diagnosis or therapy of specific cancers. Hydrophilic alkyl phosphines, in general, tend to be oxidatively unstable. Therefore, incorporation of such phosphine functionalities on peptide (and other biomolecule) backbones, without oxidizing the P.sup.III centers, is difficult. In this context this discovery reports on a new technology by which phosphines, in the form of bifunctional chelating agents, can be directly incorporated on biomolecular backbones using manual synthetic or solid phase peptide synthesis methodologies. The superior ligating abilities of phosphine ligands, with various diagnostically (e.g. TC-99m) or therapeutically (e.g. Re186/188, Rh-105, Au-199) useful radiometals, coupled with the findings that the resulting complexes demonstrate high in vivo stability makes this approach useful in the development of radiolabeled biomolecules for applications in the design of tumor-specific radiopharmaceuticals.

  20. Cellular content of biomolecules in sub-seafloor microbial communities (United States)

    Braun, Stefan; Morono, Yuki; Becker, Kevin W.; Hinrichs, Kai-Uwe; Kjeldsen, Kasper U.; Jørgensen, Bo B.; Lomstein, Bente Aa.


    Microbial biomolecules, typically from the cell envelope, can provide crucial information about distribution, activity, and adaptations of sub-seafloor microbial communities. However, when cells die these molecules can be preserved in the sediment on timescales that are likely longer than the lifetime of their microbial sources. Here we provide for the first time measurements of the cellular content of biomolecules in sedimentary microbial cells. We separated intact cells from sediment matrices in samples from surficial, deeply buried, organic-rich, and organic-lean marine sediments by density centrifugation. Amino acids, amino sugars, muramic acid, and intact polar lipids were analyzed in both whole sediment and cell extract, and cell separation was optimized and evaluated in terms of purity, separation efficiency, taxonomic resemblance, and compatibility to high-performance liquid chromatography and mass spectrometry for biomolecule analyses. Because cell extracts from density centrifugation still contained considerable amounts of detrital particles and non-cellular biomolecules, we further purified cells from two samples by fluorescence-activated cell sorting (FACS). Cells from these highly purified cell extracts had an average content of amino acids and lipids of 23-28 fg cell-1 and 2.3 fg cell-1, respectively, with an estimated carbon content of 19-24 fg cell-1. In the sediment, the amount of biomolecules associated with vegetative cells was up to 70-fold lower than the total biomolecule content. We find that the cellular content of biomolecules in the marine subsurface is up to four times lower than previous estimates. Our approach will facilitate and improve the use of biomolecules as proxies for microbial abundance in environmental samples and ultimately provide better global estimates of microbial biomass.

  1. Extracting folding landscape characteristics of biomolecules using mechanical forces



    In recent years single molecule force spectroscopy has opened a new avenue to provide profiles of the complex energy landscape of biomolecules. In this field, quantitative analyses of the data employing sound theoretical models, have played a major role in interpreting data and anticipating outcomes of experiments. Here, we explain how by using temperature as a variable in mechanical unfolding of biomolecules in force spectroscopy, the roughness of the energy landscape can be measured without...

  2. Miniature Laboratory for Detecting Sparse Biomolecules (United States)

    Lin, Ying; Yu, Nan


    A miniature laboratory system has been proposed for use in the field to detect sparsely distributed biomolecules. By emphasizing concentration and sorting of specimens prior to detection, the underlying system concept would make it possible to attain high detection sensitivities without the need to develop ever more sensitive biosensors. The original purpose of the proposal is to aid the search for signs of life on a remote planet by enabling the detection of specimens as sparse as a few molecules or microbes in a large amount of soil, dust, rocks, water/ice, or other raw sample material. Some version of the system could prove useful on Earth for remote sensing of biological contamination, including agents of biological warfare. Processing in this system would begin with dissolution of the raw sample material in a sample-separation vessel. The solution in the vessel would contain floating microscopic magnetic beads coated with substances that could engage in chemical reactions with various target functional groups that are parts of target molecules. The chemical reactions would cause the targeted molecules to be captured on the surfaces of the beads. By use of a controlled magnetic field, the beads would be concentrated in a specified location in the vessel. Once the beads were thus concentrated, the rest of the solution would be discarded. This procedure would obviate the filtration steps and thereby also eliminate the filter-clogging difficulties of typical prior sample-concentration schemes. For ferrous dust/soil samples, the dissolution would be done first in a separate vessel before the solution is transferred to the microbead-containing vessel.

  3. Multi-state modeling of biomolecules.

    Directory of Open Access Journals (Sweden)

    Melanie I Stefan


    Full Text Available Multi-state modeling of biomolecules refers to a series of techniques used to represent and compute the behavior of biological molecules or complexes that can adopt a large number of possible functional states. Biological signaling systems often rely on complexes of biological macromolecules that can undergo several functionally significant modifications that are mutually compatible. Thus, they can exist in a very large number of functionally different states. Modeling such multi-state systems poses two problems: the problem of how to describe and specify a multi-state system (the "specification problem" and the problem of how to use a computer to simulate the progress of the system over time (the "computation problem". To address the specification problem, modelers have in recent years moved away from explicit specification of all possible states and towards rule-based formalisms that allow for implicit model specification, including the κ-calculus, BioNetGen, the Allosteric Network Compiler, and others. To tackle the computation problem, they have turned to particle-based methods that have in many cases proved more computationally efficient than population-based methods based on ordinary differential equations, partial differential equations, or the Gillespie stochastic simulation algorithm. Given current computing technology, particle-based methods are sometimes the only possible option. Particle-based simulators fall into two further categories: nonspatial simulators, such as StochSim, DYNSTOC, RuleMonkey, and the Network-Free Stochastic Simulator (NFSim, and spatial simulators, including Meredys, SRSim, and MCell. Modelers can thus choose from a variety of tools, the best choice depending on the particular problem. Development of faster and more powerful methods is ongoing, promising the ability to simulate ever more complex signaling processes in the future.

  4. 装备再制造设计及其内容体系%Equipment Remanufacturing Design and Its Content System

    Institute of Scientific and Technical Information of China (English)

    朱胜; 姚巨坤


    装备再制造工程是实现装备资源节约与环境保护的重要手段,科学的装备再制造设计是提高装备再制造效益及提高装备再制造性的关键方法,也是再制造工程的重要研究领域.文中指出装备再制造设计面向装备再制造的全过程,包括面向生产的设计、面向回收物流的设计和面向装备服役的设计3部分内容,提出了再制造设计研究的范围、对象、目的、手段及时域,明确了其任务和目标,构建了包含再制造设计基础、再制造技术与工艺设计、再制造管理设计的装备再制造设计理论与技术体系,并确立了装备再制造设计要采取全系统、全寿命、全费用和绿色化的基本观点,给出了今后发展的装备再制造性设计与评价、再制造生产系统规划设计、再制造逆向物流优化设计、再制造信息管理与应用设计等4个关键领域的研究目标和内容.%Equipment remanufacturing is an important approach to realize the equipment resource saving and environment protection, and developing the scientific remanufacturing design is the key method to enhance the remanufacturing benefit and increase the remanufacturability, so it has become the important research field for remanufacturing engineering. It is introduced in this paper that the equipment remanufacturing design faces the whole process of remanufacturing, and includes three parts of design facing production: design facing reverse logistics and design facing equipment service. Some questions, such as the scopes, object, target, measure, time field, task and goal about remanufacturing design, have been studied and defined in this paper, also construct the remanufacturing design system which consists of the remanufacturing design, remanufacturing technology and process design, remanufacturing management design, and brings forward that should adopt total system, total life, total cost and green point in remanufacturing design

  5. 电子设备机箱电磁屏蔽分析和设计%Electronic Equipment Chassis Electromagnetic Shielding Analysis and Design

    Institute of Scientific and Technical Information of China (English)



    On the analysis of the electronic equipment working environment,and on the basis of the electronic equipment of external clear main interference source,and shielding effectiveness analysis.For improving Electronic Equipments' resist-disturbance capability,contraposing outside interference source,advance shielding filtering and ground and otherwise resist-disturbance design method,to insure Electronic Equipments can be working up to snuff in the condition of complex execrable war field EME.%在分析了电子设备工作环境的基础上,明确了电子设备外部的主要干扰源,并进行了屏蔽效能分析。为提高电子设备抗干扰性能,针对外部干扰源提出了屏蔽、滤波和接地等抗干扰设计方案,确保电子设备能在复杂恶劣的环境下正常工作。

  6. A New Study of Design of Equipment for Firefighters Promptly Involved%一种新型消防快速出警设备的设计

    Institute of Scientific and Technical Information of China (English)



    设计了一种便于消防快速出警的设备.设备主要由轮轴、滑索及弹簧等部件构成.该设备在确保消防队员及时出警的同时,也克服了传统的消防滑竿的种种安全缺陷.该设备造价低廉,制造工艺简单,不仅可以应用在消防站,也可以应用在石油化工等需要替代滑竿的企业场所.设备具有较强的市场推广应用价值.%In this paper, an equipment to ensure firefighters promptly get involved is designed. It consists of a wheel、 a slip cable and springs and other components. The equipment not only ensures firefighters promptly get involved, but also overcomes safety deficiencies of traditional fire pole. At the same time, the equipment has great value to popularizing its market because it is low in cost, simple manufacturing process. So it can be used in the fire station, and also in petrochemical enterprises or other places where need replace fire pole.

  7. One-step 18F labeling of biomolecules using organotrifluoroborates (United States)

    Liu, Zhibo; Lin, Kuo-Shyan; Bénard, François; Pourghiasian, Maral; Kiesewetter, Dale O; Perrin, David M; Chen, Xiaoyuan


    Herein we present a general protocol for the functionalization of biomolecules with an organotrifluoroborate moiety so that they can be radiolabeled with aqueous 18F fluoride (18F−) and used for positron emission tomography (PET) imaging. Among the β+-emitting radionuclides, fluorine-18 (18F) is the isotope of choice for PET, and it is produced, on-demand, in many hospitals worldwide. Organotrifluoroborates can be 18F-labeled in one step in aqueous conditions via 18F–19F isotope exchange. This protocol features a recently designed ammoniomethyltrifluoroborate, and it describes the following: (i) a synthetic strategy that affords modular synthesis of radiolabeling precursors via a copper-catalyzed ‘click’ reaction; and (ii) a one-step 18F-labeling method that obviates the need for HPLC purification. Within 30 min, 18F-labeled PET imaging probes, such as peptides, can be synthesized in good chemical and radiochemical purity (>98%), satisfactory radiochemical yield of 20–35% (n > 20, non-decay corrected) and high specific activity of 40–111 GBq/µmol (1.1–3.0 Ci/µmol). The entire procedure, including the precursor preparation and 18F radiolabeling, takes 7–10 d. PMID:26313478


    Institute of Scientific and Technical Information of China (English)

    龚炳江; 黄彦欣; 贾海鑫


    通过对主题网络爬虫的研究,设计一个对网络中的矿山设备资源进行收集的主题网络爬虫。设计内容主要包括主题网络爬虫的各个功能模块以及各功能模块实现的方法。例如判定网页主题相关度及URL价值评价等。使用的主要技术为向量空间模型和PageRank算法。矿山设备领域主题网络爬虫的研究与设计为矿山设备领域主题爬虫的实现奠定了基础。%Through the research on topic web crawler, we design a topic web crawler which can collect the mining equipments resources in networks.The content of the design mainly includes each functional module of the topic web crawler and the method to realise these function modules.For example, to judge the correlation of webpage topics and the URL value evaluation, and so on.The main technology it used is the vector space model and the PageRank algorithm.The study and design of the topic crawler for mining equipments field lays a sound foundation for its implementation.

  9. Database design on accounting treatment system for music equipment management%音乐器材管理账务处理系统数据库设计

    Institute of Scientific and Technical Information of China (English)



    音乐教育是我国素质教育的重要组成部分,提高学生的音乐艺术素养,必须充分发挥音乐器材的重要作用。针对音乐器材账务难以管理的现状,本文进行数据库设计,为账务处理系统开发提供支持。以需求分析为基础,进行了概念结构设计和逻辑结构设计。需求分析采用UML进行用例建模,概念结构设计采用实体联系图表示,逻辑结构设计以转换规则为基础通过表格形式设计了3个表结构。%Music education is an important part of quality education,which can improve their music art accomplishment,so the important role of music equipment must be fully play.This paper studies database design in the light of the present situation of music equipment accounting difficult to manage to provide support for the development of accounting treatment system.Conceptual structure design and logical structure design should be carried on based on requirement analysis.Requirement analysis conducted use case modeling used UML;conceptual structure design was showed by the diagram of entity-relationship;logical structure design designed three table structures by the table form based on transformation rule.

  10. 通用航空RDSS机载设备研究及设计%Research and Design on General Aviation RDSS Airborne Equipment

    Institute of Scientific and Technical Information of China (English)



    本文设计了一套集成RDSS卫星通信和3G/4G数据通信的机载设备,实现通用航空飞行器的应急通信与定位功能,提供低成本多通道的数据通信,强化了飞行器的安全保障,补充通用航空空域管制的覆盖区域。设备具备体积小,集成度高,可以根据用户需求自定制,具有较高的可扩展性和可裁剪性,电源部分采用软开关控制方式,具有较强的可靠性和可维护性。%This paper designs a set of airborne equipment integrated with RDSS satellite communication and 3G/4G data communication. This airborne equipment realizes the function of emergency communications and positioning of common aero vehicle, provides the data communication of low cost and multi channels, and strengthens the security of aircraft, supplements the coverage of general aviation airspace control. This equipment has a small size and high integration, and it can be customized according to the user needs, and has the ability of high expansibility and scalability. The power part of RDSS airborne equipment adopts soft switch control mode, and has the strong reliability and maintainability.

  11. Strategies to balance covalent and non-covalent biomolecule attachment within collagen-GAG biomaterials. (United States)

    Pence, Jacquelyn C; Gonnerman, Emily A; Bailey, Ryan C; Harley, Brendan A C


    Strategies to integrate instructive biomolecular signals into a biomaterial are becoming increasingly complex and bioinspired. While a large majority of reports still use repeated treatments with soluble factors, this approach can be prohibitively costly and difficult to translate in vivo for applications where spatial control over signal presentation is necessary. Recent efforts have explored the use of covalent immobilization of biomolecules to the biomaterial, via both bulk (ubiquitous) as well as spatially-selective light-based crosslinking, as a means to both enhance stability and bioactivity. However, little is known about how processing conditions during immobilization impact the degree of unintended non-covalent interactions, or fouling, that takes place between the biomaterial and the biomolecule of interest. Here we demonstrate the impact of processing conditions for bulk carbodiimide (EDC) and photolithography-based benzophenone (BP) crosslinking on specific attachment vs. fouling of a model protein (Concanavalin A, ConA) within collagen-glycosaminoglycan (CG) scaffolds. Collagen source significantly impacts the selectivity of biomolecule immobilization. EDC crosslinking intensity and ligand concentration significantly impacted selective immobilization. For benzophenone photoimmobilization we observed that increased UV exposure time leads to increased ConA immobilization. Immobilization efficiency for both EDC and BP strategies was maximal at physiological pH. Increasing ligand concentration during immobilization process led to enhanced immobilization for EDC chemistry, no impact on BP immobilization, but significant increases in non-specific fouling. Given recent efforts to covalently immobilize biomolecules to a biomaterial surface to enhance bioactivity, improved understanding of the impact of crosslinking conditions on selective attachment versus non-specific fouling will inform the design of instructive biomaterials for applications across tissue

  12. Design and Implementation of Wearable ECG Monitoring Equipment%可穿戴心电监护设备设计与实现

    Institute of Scientific and Technical Information of China (English)

    祝宇虹; 牛群


    为了满足心脏病患者日益增长的心电监护需求,设计了一种可穿戴式的心电监护设备来满足患者日常生活的心电监护功能。设备设计包括硬件和软件2部分,硬件方面采用柔性织物电极、AD8232芯片和STM32微控制器实现对心电信号的采集、A/D转换和传输功能;软件方面借用小波变换在心电信号去噪和识别方面的优势,获得清晰的心电信号和心率,并实现对症状的初步诊断;最后设计了基于 LabVIEW平台的友好人机交互界面,可以直观、清楚的显示心电信号的实时波形,当前患者的R R间期和心率参数。利用本设备对几位志愿者进行测试,得到了良好的结果,可以实现患者日常心电监护。%In order to meet the growing demand of patients with heart diseases for ECG monitoring, this paper designs wearable ECG monitoring equipment to achieve the ECG monitoring function of daily life for patients.The equipment design comprises hardware and software design.In terms of hardware,the flexible fabric electrode,AD8232 chip and STM32 microcontroller are used to implement the functions of ECG signal acquisition,A/D conversion and transmission;In term of software,the advantages of the wavelet transformation in ECG signal denoising and identification are utilized to obtain a clear ECG signal and heart rate,and realize the preliminary diagnosis of the symptoms.Finally,a friendly human computer inter-action interface based on the LabVIEW platform can display real time ECG signal waveform,the current RR inter phase and the heart rate in patients intuitively and clearly.Several volunteers are tested with the equipment. Good results are obtained.The equipment can realize patients'daily ECG monitoring.

  13. Design Points of New Civil Air Defense Equipment in Subway Sections%地铁区间新型人防设备设计要点

    Institute of Scientific and Technical Information of China (English)

    郭士博; 徐胜


    经济合理地实现城市轨道交通设计中的人防功能是地铁设计的一个重要问题。为解决地铁区间刚性接触网不断开条件下的人防设防问题,设计出新型区间单扇防护密闭隔断门。新型设备同时有效地解决了线路曲线段、大坡段及大盾构区间施工段的人防设防问题。详细介绍了新型设备的机构设计原理、工作性能及既有难题的解决方案,对其与既有设备的改进处进行对比,得出了新型设备的优越性与应用范围。%How to realize the functions of air defense eco-nomically and rationaly is an important issue in subway de-sign.Airtight blast partition door is designed for rigid cate-nary subway system which can be used when the catenaries and rail track are not disconnected.This new equipment al-so effectively solves the problem of air defense fortification line curve segments,steep slopes and large segments in shield zone construction.In this paper,the new equipment design principles, performance and solutions to existing problems are introduced in detail.The improvement and application range of the new equipment are also described.

  14. Anthropometric study of farm workers on Java Island, Indonesia, and its implications for the design of farm tools and equipment. (United States)

    Syuaib, M Faiz


    Anthropometric data are a prerequisite for designing agricultural tools and equipment that enable workers to achieve better performance and productivity while providing better safety and comfort. A set of thirty anthropometric dimensions was collected from a total sample of 371 male and female farm-workers from three different regions (west, central and east) of Java Island, Indonesia. The mean stature is 162.0 cm and 152.5 cm, the sitting height is 82.9 cm and 77.4 cm, and the body weight is 57.1 kg and 52.3 kg for male and female subjects, respectively. The index of relative sitting height (RSH) was 0.51 on average for both male and female subjects. Significant differences are found in most of the anthropometric dimensions between gender and regional data groups as well. Compared with groups of people from several other countries, the anthropometric dimensions of Indonesian people are quite similar to Indian people, but are relatively smaller than Filipino, Chinese, Japanese, British, and American people. An attempt was conducted to illustrate the use of this anthropometric database and ergonomic considerations in refining the design of traditional tools and equipment commonly in use for rice farming operations.

  15. Characterization data needs for development, design, and operation of retrieval equipment developed through the data quality objective process

    Energy Technology Data Exchange (ETDEWEB)

    Bloom, G.R., Westinghouse Hanford


    This Data Quality Objective identifies the specific characterization data required to support the development,design, and operation of systems to retrieve and transfer to storage waste from both single- shell tanks and double-shell tanks.

  16. Comprehensive design and optimization of an electric vehicle powertrain equipped with a two-speed dual-clutch transmission

    Directory of Open Access Journals (Sweden)

    Yu Wang


    Full Text Available This article develops a systematic model to study electric vehicle powertrain system efficiency by combining a detailed model of two-speed dual-clutch transmission system efficiency losses with an electric vehicle powertrain system model. In this model, the design factors including selection of the electric machine, gear ratios’ change, multi-plate wet clutch design, and gear shift schedule design are considered. Meanwhile, the application of detailed model for drag torque losses in the gearbox is discussed. Furthermore, the proposed model, developed with the MATLAB/Simulink platform, is applied to optimize/maximize the efficiency of the electric vehicle powertrain system using genetic algorithms. The optimization results demonstrate that the optimal results are different between simulations via New Europe Drive Cycle and Urban Dynamometer Driving Schedule, and comprehensive design and optimization of the powertrain system are necessary.

  17. Design of intelligent monitoring system for medical equipment%医疗设备智能监控系统的设计

    Institute of Scientific and Technical Information of China (English)

    赵明; 奚卫东


    Objective: Intelligent monitoring system of medical equipment can realize the intelligent dynamic management to equipment in the hospital wireless or wired network environment. Methods: Use of communication network, intelligent call, system monitoring and other means of science and technology to design the intelligent micro device state sensor. Results:System is mainly for hospital equipment, which can capture and management of medical equipment data. When the elderly have abnormal condition, it can send information through intelligent system, through identification and location, to realize the construction of equipment state information. Conclusion: Intelligent sensor in the system application can achieve the sharing of resources; realize the whole hand or full coverage of the intelligent health network system, providing an effective guarantee for the health of the masses.%目的:建立医疗设备智能监控系统,使医院在无线覆盖或有线网络的环境下,实现设备的智能化动态管理。方法:利用通讯网络、智能呼叫及系统监测等科技手段,设计医疗设备智能状态传感器。结果:针对医院医疗设备,将其数据进行采集和管理。当出现异常状况时可通过智能系统发出相关信息,通过身份识别与定位,实现设备状态的信息化。结论:智能传感器在系统的运用中可做到资源共享,实现了全方位、全覆盖的智能医院物联网系统,为医院的医疗服务提供了有效的保障。

  18. Tools and procedures for visualization of proteins and other biomolecules. (United States)

    Pan, Lurong; Aller, Stephen G


    Protein, peptides, and nucleic acids are biomolecules that drive biological processes in living organisms. An enormous amount of structural data for a large number of these biomolecules has been described with atomic precision in the form of structural "snapshots" that are freely available in public repositories. These snapshots can help explain how the biomolecules function, the nature of interactions between multi-molecular complexes, and even how small-molecule drugs can modulate the biomolecules for clinical benefits. Furthermore, these structural snapshots serve as inputs for sophisticated computer simulations to turn the biomolecules into moving, "breathing" molecular machines for understanding their dynamic properties in real-time computer simulations. In order for the researcher to take advantage of such a wealth of structural data, it is necessary to gain competency in the use of computer molecular visualization tools for exploring the structures and visualizing three-dimensional spatial representations. Here, we present protocols for using two common visualization tools--the Web-based Jmol and the stand-alone PyMOL package--as well as a few examples of other popular tools.

  19. Temperature-responsive chromatography for the separation of biomolecules. (United States)

    Kanazawa, Hideko; Okano, Teruo


    Temperature-responsive chromatography for the separation of biomolecules utilizing poly(N-isopropylacrylamide) (PNIPAAm) and its copolymer-modified stationary phase is performed with an aqueous mobile phase without using organic solvent. The surface properties and function of the stationary phase are controlled by external temperature changes without changing the mobile-phase composition. This analytical system is based on nonspecific adsorption by the reversible transition of a hydrophilic-hydrophobic PNIPAAm-grafted surface. The driving force for retention is hydrophobic interaction between the solute molecules and the hydrophobized polymer chains on the stationary phase surface. The separation of the biomolecules, such as nucleotides and proteins was achieved by a dual temperature- and pH-responsive chromatography system. The electrostatic and hydrophobic interactions could be modulated simultaneously with the temperature in an aqueous mobile phase, thus the separation system would have potential applications in the separation of biomolecules. Additionally, chromatographic matrices prepared by a surface-initiated atom transfer radical polymerization (ATRP) exhibit a strong interaction with analytes, because the polymerization procedure forms a densely packed polymer, called a polymer brush, on the surfaces. The copolymer brush grafted surfaces prepared by ATRP was an effective tool for separating basic biomolecules by modulating the electrostatic and hydrophobic interactions. Applications of thermally responsive columns for the separations of biomolecules are reviewed here.

  20. 46 CFR 197.460 - Diving equipment. (United States)


    ... 46 Shipping 7 2010-10-01 2010-10-01 false Diving equipment. 197.460 Section 197.460 Shipping COAST... GENERAL PROVISIONS Commercial Diving Operations Periodic Tests and Inspections of Diving Equipment § 197.460 Diving equipment. The diving supervisor shall insure that the diving equipment designated for...

  1. 7 CFR 58.626 - Packaging equipment. (United States)


    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Packaging equipment. 58.626 Section 58.626 Agriculture....626 Packaging equipment. Packaging equipment designed to mechanically fill and close single service... Standards for Equipment for Packaging Frozen Desserts and Cottage Cheese. Quality Specifications for...

  2. 某机载电子设备总体结构设计%Structure Design of Airborne Electronic Equipment for a Certain Aircraft

    Institute of Scientific and Technical Information of China (English)

    南雁; 郭建平; 张娅妮


    从结构设计角度出发,根据航空平台的工作模式和环境空间要求,针对某机载电子设备的标准化设计、热设计、电磁兼容性设计、防冲击、振动、"三防"设计等方面进行了论述.结合型号设计的工程经验,以航空电子设备结构设计的特点和原则为重点,对机载电子设备的结构设计进行了阐述,通过合理的布局设计和仿真计算来实现结构设计要求.介绍机载电子设备结构设计的经验,可供同行参考.%From the point of structure design,according to work and environment requisition for the aircraft flat ,described delailed such as the standardization, heat, EMC,impact and vibration prevention,three proofing designing for a certain aircraft. Focus on the characteristic and principle for aeronauticat electronic equipment,expatiate the structure design from Many- sided ,and guidance thc structure design by logical position and simulation with engineering experience. Pool the experience for structure design and offer the experience for craft brother discuss.

  3. Constraint methods that accelerate free-energy simulations of biomolecules. (United States)

    Perez, Alberto; MacCallum, Justin L; Coutsias, Evangelos A; Dill, Ken A


    Atomistic molecular dynamics simulations of biomolecules are critical for generating narratives about biological mechanisms. The power of atomistic simulations is that these are physics-based methods that satisfy Boltzmann's law, so they can be used to compute populations, dynamics, and mechanisms. But physical simulations are computationally intensive and do not scale well to the sizes of many important biomolecules. One way to speed up physical simulations is by coarse-graining the potential function. Another way is to harness structural knowledge, often by imposing spring-like restraints. But harnessing external knowledge in physical simulations is problematic because knowledge, data, or hunches have errors, noise, and combinatoric uncertainties. Here, we review recent principled methods for imposing restraints to speed up physics-based molecular simulations that promise to scale to larger biomolecules and motions.

  4. Constraint methods that accelerate free-energy simulations of biomolecules

    Energy Technology Data Exchange (ETDEWEB)

    Perez, Alberto [Laufer Center for Physical and Quantitative Biology, Stony Brook University, Stony Brook, New York 11794 (United States); MacCallum, Justin L. [Department of Chemistry, University of Calgary, Calgary, Alberta T2N 1N4 (Canada); Coutsias, Evangelos A. [Laufer Center for Physical and Quantitative Biology, Stony Brook University, Stony Brook, New York 11794 (United States); Department of Applied Mathematics, Stony Brook University, Stony Brook, New York 11794 (United States); Dill, Ken A. [Laufer Center for Physical and Quantitative Biology, Stony Brook University, Stony Brook, New York 11794 (United States); Department of Physics and Astronomy, Stony Brook University, Stony Brook, New York 11794 (United States)


    Atomistic molecular dynamics simulations of biomolecules are critical for generating narratives about biological mechanisms. The power of atomistic simulations is that these are physics-based methods that satisfy Boltzmann’s law, so they can be used to compute populations, dynamics, and mechanisms. But physical simulations are computationally intensive and do not scale well to the sizes of many important biomolecules. One way to speed up physical simulations is by coarse-graining the potential function. Another way is to harness structural knowledge, often by imposing spring-like restraints. But harnessing external knowledge in physical simulations is problematic because knowledge, data, or hunches have errors, noise, and combinatoric uncertainties. Here, we review recent principled methods for imposing restraints to speed up physics-based molecular simulations that promise to scale to larger biomolecules and motions.

  5. Kinetic analysis of interaction between lipopolysaccharide and biomolecules

    Institute of Scientific and Technical Information of China (English)

    Fan YANG; Xiurong YANG


    Lipopolysaccharide (LPS) is a major compo-nent of the outer membrane of all gram-negative bacteria. It interacts with some biomolecules and triggers a toxic reaction. In this paper, we studied the interaction between LPS from Salmonella Minnesota and some biomolecules using a surface plasmon resonance (SPR) biosensor. Biomolecules were immobilized on a CM5 sensor chip using the amino coupling method and LPS was injected over the immobilized surfaces. The affinity constant KA of LPS with serum albumin, hemoglobin, chitosan and lysozyme was 2.36 × 107, 2.03 × 108,7.58×106, 2.82 × 104 L·mol-1, respectively. However, LPS could not interact with ferritin.

  6. Printing technologies for biomolecule and cell-based applications. (United States)

    Ihalainen, Petri; Määttänen, Anni; Sandler, Niklas


    Biomolecules, such as enzymes, proteins and other biomacromolecules (polynucleotides, polypeptides, polysaccharides and DNA) that are immobilized on solid surfaces are relevant to many areas of science and technology. These functionalized surfaces have applications in biosensors, chromatography, diagnostic immunoassays, cell culturing, DNA microarrays and other analytical techniques. Printing technologies offer opportunities in this context. The main interests in printing biomolecules are in immobilizing them on surfaces for sensors and catalysts or for controlled delivery of protein-based drugs. Recently, there have been significant developments in the use of inkjet printing for dispensing of proteins, biomacromolecules and cells. This review discusses the use of roll-to-roll and inkjet printing technologies in manufacturing of biomolecule and cell-based applications.

  7. Understanding small biomolecule-biomaterial interactions: a review of fundamental theoretical and experimental approaches for biomolecule interactions with inorganic surfaces. (United States)

    Costa, Dominique; Garrain, Pierre-Alain; Baaden, Marc


    Interactions between biomolecules and inorganic surfaces play an important role in natural environments and in industry, including a wide variety of conditions: marine environment, ship hulls (fouling), water treatment, heat exchange, membrane separation, soils, mineral particles at the earth's surface, hospitals (hygiene), art and buildings (degradation and biocorrosion), paper industry (fouling) and more. To better control the first steps leading to adsorption of a biomolecule on an inorganic surface, it is mandatory to understand the adsorption mechanisms of biomolecules of several sizes at the atomic scale, that is, the nature of the chemical interaction between the biomolecule and the surface and the resulting biomolecule conformations once adsorbed at the surface. This remains a challenging and unsolved problem. Here, we review the state of art in experimental and theoretical approaches. We focus on metallic biomaterial surfaces such as TiO(2) and stainless steel, mentioning some remarkable results on hydroxyapatite. Experimental techniques include atomic force microscopy, surface plasmon resonance, quartz crystal microbalance, X-ray photoelectron spectroscopy, fluorescence microscopy, polarization modulation infrared reflection absorption spectroscopy, sum frequency generation and time of flight secondary ion mass spectroscopy. Theoretical models range from detailed quantum mechanical representations to classical forcefield-based approaches.

  8. Design and testing of Ground Penetrating Radar equipment dedicated for civil engineering applications: ongoing activities in Working Group 1 of COST Action TU1208 (United States)

    Pajewski, Lara; Manacorda, Guido; Persico, Raffaele


    This work aims at presenting the ongoing research activities carried out in Working Group 1 'Novel GPR instrumentation' of the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar' ( The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Working Group 1 (WG1) of the Action focuses on the development of innovative GPR equipment dedicated for civil engineering applications. It includes three Projects. Project 1.1 is focused on the 'Design, realisation and optimisation of innovative GPR equipment for the monitoring of critical transport infrastructures and buildings, and for the sensing of underground utilities and voids.' Project 1.2 is concerned with the 'Development and definition of advanced testing, calibration and stability procedures and protocols, for GPR equipment.' Project 1.3 deals with the 'Design, modelling and optimisation of GPR antennas.' During the first year of the Action, WG1 Members coordinated between themselves to address the state of the art and open problems in the scientific fields identified by the above-mentioned Projects [1, 2]. In carrying our this work, the WG1 strongly benefited from the participation of IDS Ingegneria dei Sistemi, one of the biggest GPR manufacturers, as well as from the contribution of external experts as David J. Daniels and Erica Utsi, sharing with the Action Members their wide experience on GPR technology and methodology (First General Meeting, July 2013). The synergy with WG2 and WG4 of the Action was useful for a deep understanding of the problems, merits and limits of available GPR equipment, as well as to discuss how to quantify the reliability of GPR results. An

  9. Design and Research of GPS/BDS Airborne Equipment in General Aviation%通用航空GPS/BDS机载设备研究及设计

    Institute of Scientific and Technical Information of China (English)

    贺星; 方成


    本文描述了 GPS/BDS 机载设备的总体架构和详细设计方法。基带处理部分,给出了基于PMF-FFT的捕获算法。基带处理电路主要完成对射频通道传送过来的中频信号进行采样、数字滤波、信号处理、功能解算等功能。GPS/BDS 机载设备具备兼容 GPS、BDS 和 GLONASS卫星导航系统的能力;具备卫星导航系统历书、星历、原始测量数据输出、RAIM处理、差分处理、多种数据格式输出、BIT系统自检、工作状态提示等能力。%This paper describes the structure and design method in detail for the GPS/BDS of airborne equipment. In the baseband processing parts, the acquisition algorithm based on PMF-FFT is presented. Baseband processing circuit mainly complete the collection, the digital filtering, the signal processing and function calculating of intermediate frequency signal that from RF channel. GPS airborne equipment has the ability of satellite navigation system, meanwhile has the capability of compatible with GPS, BDS and GLONASS. The equipment has ability of receiving almanac and ephemeris of satellite navigation system, the original measurement data output, RAIM processing, difference processing, a variety of data format output, BIT system self-checking, status display and so on.

  10. Design of ZigBee-Based RFID Svstem in Equipment Management%基于ZigBee技术的RFID装备管理系统设计

    Institute of Scientific and Technical Information of China (English)

    郭瑞; 李胤; 王永超; 刘久文


    提出了一种基于ZigBee技术的、用于装备管理的RFID系统设计方法.由于ZigBee组网技术具有很好的可靠性、稳定性和安全性,并且具有一定的自组织和自适应性,将ZigBee技术用于RFID系统中,提高了RFID系统组建的便捷性以及数据传输的可靠性和安全性,有效地满足了装备管理的各项需求.另外,将ZigBee部分网络节点作为特殊标签,利用无线网络节点的各项信息,可以完成对这些特殊节点的定位,从而加强了对特殊装备的监控和管理,进一步扩展了RFID系统的功能.%A design of RFID system in equipment management by applying ZigBee technology is proposed. Because of the stability, reliability, security of ZigBee networking technology which also has a certain degree of self-organization and adaptability, the established RFID system using ZigBee technology is more convenient and also the communication in RFID system is more reliable and secure. Consequently, each requirement of equipment management is satisfied more effectively. Furthermore, by applying the ZigBee network node as a special tag which can be located by the carried information of wireless network, some important equipment with such tag can be monitored and managed in a higher level and the functions of RFID system are also expanded.

  11. Design of automatic control system for antigravity casting equipment%反重力铸造设备自动控制系统的设计

    Institute of Scientific and Technical Information of China (English)

    王艳珍; 邹蕾; 朱登飙


    According to the actual control needs of the antigravity casting equipment,an automatic control system for anti⁃gravity casting equipment was designed by means of modern automatic control technology. The overall design of the system,hard⁃ware selection and software design were accomplished. The digital PID control method is used to improve the system’s self⁃com⁃pensation ability and anti⁃interference ability. The Ziegler⁃Nichols method is used to set the digital PID parameters. The setting results were tested with the heat adjustment test. The test results show that the system has reliable operation,good curve tracking performance,and can achieve the application requirement.%根据反重力铸造设备实际控制的需要,运用现代自动控制技术设计一种反重力铸造设备自动控制系统,完成系统的总体设计、硬件选型与软件设计。运用数字PID控制方法对提高系统自补偿能力与抗干扰能力,通过Ziegler⁃Nichols方法对数字PID参数进行整定,对结果通过热调试验进行测试。结果表明,系统运行可靠,曲线跟踪性能良好,达到使用要求。

  12. Quantum chemistry and charge transport in biomolecules with superconducting circuits (United States)

    García-Álvarez, L.; Las Heras, U.; Mezzacapo, A.; Sanz, M.; Solano, E.; Lamata, L.


    We propose an efficient protocol for digital quantum simulation of quantum chemistry problems and enhanced digital-analog quantum simulation of transport phenomena in biomolecules with superconducting circuits. Along these lines, we optimally digitize fermionic models of molecular structure with single-qubit and two-qubit gates, by means of Trotter-Suzuki decomposition and Jordan-Wigner transformation. Furthermore, we address the modelling of system-environment interactions of biomolecules involving bosonic degrees of freedom with a digital-analog approach. Finally, we consider gate-truncated quantum algorithms to allow the study of environmental effects.

  13. Quantum chemistry and charge transport in biomolecules with superconducting circuits. (United States)

    García-Álvarez, L; Las Heras, U; Mezzacapo, A; Sanz, M; Solano, E; Lamata, L


    We propose an efficient protocol for digital quantum simulation of quantum chemistry problems and enhanced digital-analog quantum simulation of transport phenomena in biomolecules with superconducting circuits. Along these lines, we optimally digitize fermionic models of molecular structure with single-qubit and two-qubit gates, by means of Trotter-Suzuki decomposition and Jordan-Wigner transformation. Furthermore, we address the modelling of system-environment interactions of biomolecules involving bosonic degrees of freedom with a digital-analog approach. Finally, we consider gate-truncated quantum algorithms to allow the study of environmental effects.

  14. Quantum chemistry and charge transport in biomolecules with superconducting circuits (United States)

    García-Álvarez, L.; Las Heras, U.; Mezzacapo, A.; Sanz, M.; Solano, E.; Lamata, L.


    We propose an efficient protocol for digital quantum simulation of quantum chemistry problems and enhanced digital-analog quantum simulation of transport phenomena in biomolecules with superconducting circuits. Along these lines, we optimally digitize fermionic models of molecular structure with single-qubit and two-qubit gates, by means of Trotter-Suzuki decomposition and Jordan-Wigner transformation. Furthermore, we address the modelling of system-environment interactions of biomolecules involving bosonic degrees of freedom with a digital-analog approach. Finally, we consider gate-truncated quantum algorithms to allow the study of environmental effects. PMID:27324814

  15. 静态RCS测量雷达的设备布局及升降塔结构设计%Equipment Layout Design of Static RCS Measure Radar and Structure Design of Lift Tower

    Institute of Scientific and Technical Information of China (English)

    张兴唐; 李志兵; 马萍


    文中对静态RCS全频段测量雷达多天线和多收发设备的布局进行了设计,针对结构设计的核心部分——可移动高架天线塔进行了详细论述,对升降塔风栽荷进行了分析计算并用有限元法校核了天线塔身和天线安装架的强度,结果满足结构设计强度要求。解决了高架天线塔升降、天线安装调整、天线塔所装设备维修性和安全性的问题。对多波段静态RCS测量设备总体布局设计和高架升降塔的设计具有参考借鉴意义。%This paper designs the layout of antennas and T/R equipments for whole frequency bands static RCS measure radar. Detailed discussion is per formed for the mobile antenna tower which is the key part of structure design. Wind-load computing is carried out for the lift tower, the strength of antenna tower and an- tenna mounting structure is checked by finite element method, the results meet the design requirements. The problems of antenna going up and down, antenna stance adjusting, maintenance and security of equipments in antenna tower are solved in this paper. Study results of this paper are helpful to the layout design of multi- bands static RCS measure equipments and the structure design of lift tower.

  16. Design and simulation of heat pumps and A/C equipment using pure and mixed refrigerants with MoMo (Modular Modelling)

    Energy Technology Data Exchange (ETDEWEB)

    Bensafi, A. [CETIAT, Centre Technique des Industries Aerauliques et Thermiques, 69 - Villeurbanne (France)


    An approach to the steady-state simulation of A/C, heat pumping and refrigerating equipment is presented. Novel features of the proposed model are the strategy and formalism adopted. Individual mathematical models for component design are dynamically linked to enable the simulation of a vapour compression cycle. This paper introduces some of the component modules, which can be used for pure as well as mixed refrigerants. Because numerous models can be used for specific pieces of equipment making up the whole refrigerating/heating unit, the Modular Modeling approach leads to a flexible and evolutive structure. The effects of operating conditions, refrigerant charge, or component geometrical parameters on cycle performance can then be examined. Because actual test data are used to 'calibrate' the component modules, very accurate results are obtained. The proposed model is validated with experimental results. Discrepancies smaller than 5% are obtained on the cooling duty and COP. An air conditioning unit using high pressure refrigerant R410A is optimised to illustrate the use of the model. (authors)

  17. Design of Assemble or Disassemble Equipment System Based on VR Technology%基于VR技术的设备拆装系统设计

    Institute of Scientific and Technical Information of China (English)

    胡畔; 姜睐; 李依桐


    To solve the problem of showing and virtually operating the assemble and disassemble process of the equipments,a VR(Virtual Reality) training system based on virtual reality technology in which students can assemble or disassemble equipments was designed by a new, simple and quick software solution of "modeling software + programming language". 3DMAX software are adopted to build the virtual training scenarios and virtual devices modeling, C programming language was used to achieve display the scene and model in real-time, produce special effects and interactive control function, ultimately complete the virtual device assemble or disassembly process. After the actual use of trainees, it show that the system has the characteristics of good interaction and strong immersion, the process of assemble or disassemble is realistic, efficient and convenient,achieved its purpose of assemble or disassemble e-quipments training, and it is a preparation and complement to assemble or disassemble actual equipments.%为解决在虚拟环境中对设备拆装过程进行演示和虚拟操作的问题,设计构建基于虚拟现实技术的设备拆装实训系统,给出一种新的、简单、快捷的软件解决方案,即“模型文件十开发包”的方法.用3DMAX实现虚拟实训场景及其中虚拟设备的建模,用VC编程实现场景及模型的实时显示、特殊效果产生、人机交互控制等功能,最终实现虚拟的设备拆装实训系统设计.受训者的实际使用表明,该系统具有良好的交互性和较强的沉浸感,拆装过程逼真、高效、便捷,达到了设备拆装实训教学的训练目的,是对实物拆装实训的有益准备和补充.

  18. Engineering Design of A Gang Drilling Machine Equipped with Jig and Fixtures to Make A Prototype Machine in Birdcage Production

    Directory of Open Access Journals (Sweden)

    Eddy Widiyono


    Full Text Available This paper is dealing with the engineering design of a gang drilling machine with jig & fixtures to make a prototype machine. This effort has been done in order to solve the problem which aroused in small business enterprises producing birdcages. The problem was how to minimize the production time in making a lot of holes that have same distance and straightness. Hopefully, the prototype machine can help the small business enterprises to increase their production rate.The design engineering process has been carried out by variant approximation on dowel pin modular fixtures in order to simplify fixtures design. CAD CAM software has also been used as fixtures synthesized method including geometric analysis and three dimensional fixtures assembling. The resulting prototype machine can be well operated and based on the running test, it can be concluded that the greater the motor rotation the greater the power needed. As for teak wood, at 250 rpm motor rotation the power needed is 26.5 watt, and at 400 rpm the motor needs power of 43.6 watt while at 600 rpm the motor needs power of 600 watt. The power consumption is also depends on the type of material, the better the mechanical properties of the materials, the higher the power consumption. For cast iron, the 400 rpm motor rotation needs power as high as 569.7 watt. This prototype of gang drilling machine needs power of 350 watt to make five holes on teak wood while ordinary drilling machine needs total power of 1350 watt.

  19. 卧式连续生物炭炭化设备研制%Design and manufacture of horizontal continuous biomass carbonization equipment

    Institute of Scientific and Technical Information of China (English)

    袁艳文; 田宜水; 赵立欣; 姚宗路


    The burning of fossil fuels, the changes in agricultural and land utilization, as well as the industrial processes produce a lot of carbon dioxide and other greenhouse gases causing global warming. Climate increasingly has become one of the most far-reaching global environmental issues. The carbon can be stabilized by biochar for hundreds of years while the carbon element is very difficult to break down after mineralization. With the function of carbon sequestration and soil improvement in agriculture, it turns out to be one of the main ways to reduce carbon emissions in the future. Currently, the producing of biochar has become a hot topic both at home and abroad. In this paper, aiming at the existence of unstable operations, low percentage of biochar outputs, as well as the difficultly of recycling of by-products of China biochar, equipment designs of the horizontal equipment for continuous carbonization of biomass, which uses external thermal heating carbonization of feedstock. The difference requires an external heating fuel, the heat required for the dissertation utilizes the high temperature and gas combustion-generated calorie to carbonize the raw materials., Combustion flue gas during start-up and insufficient supply combined with diesel auxiliary heating of the equipment, the pyrolytic reactor of which is double-layer structure, with the inner layer carbonization chamber equipped with the built-in screw conveyor while the out layer high-temperature flue sleeve, using the motor speed, to ensure the continuous feeding of material, mixing and uniform heat transfer to achieve a biological continuous production of charcoal and carbonization temperature-controlled. The reactor outer sleeve for the high temperature flue gas, the use of high temperature gas to heat biomass, and the outer cylinder has baffles and fins, extending the residence time of the flue gas, and increasing the heat transfer coefficient to achieve a sufficient heat exchange. The paper chooses

  20. Aeroelastic Design and LPV Modelling of an Experimental Wind Turbine Blade equipped with Free-floating Flaps (United States)

    Navalkar, S. T.; Bernhammer, L. O.; Sodja, J.; Slinkman, C. J.; van Wingerden, J. W.; van Kuik, G. A. M.


    Trailing edge flaps located outboard on wind turbine blades have recently shown considerable potential in the alleviation of turbine lifetime dynamic loads. The concept of the free-floating flap is specifically interesting for wind turbines, on account of its modularity and enhanced control authority. Such a flap is free to rotate about its axis; camberline control of the free-floating flap allows for aeroelastic control of blade loads. This paper describes the design of a scaled wind turbine blade instrumented with free-floating flaps, intended for use in wind tunnel experiments. The nature of the flap introduces a coupled form of flutter due to the aeroelastic coupling of flap rigid-body and blade out-of-plane modes; for maximal control authority it is desired to operate close to the flutter limit. Analytical and numerical methods are used to perform a flutter analysis of the turbine blade. It is shown that the potential flow aeroelastic model can be recast as a continuous-time Linear-Parameter-Varying (LPV) state space model of a low order, for which formal controller design methodologies are readily available.

  1. The Design of the Equipment Warehouse Visual Management Information System%器材仓库可视化管理信息系统设计

    Institute of Scientific and Technical Information of China (English)

    刘大伟; 刘克胜; 王铁宁; 梁波


    The EWMIS (equipment warehouse management in- formation system) has been used extensively, it greatly im-proved the operation efficiency of the store logistics and pro- moted the improvement of the management pattern as well as the business process optimization (BPO) of the Equipment Warehouse. Now one of its main research directions is the vi- sualization of management information. Based on this objective, the reform-design of the visualization of EWMIS is finished by using the technology of virtual reality, radio frequency identifi- cation (RFID), information visualization and UML, which can establish the foundation for the warehouse management and also offer some reference on the design of the visual management information system.%器材仓库管理信息系统在现代军事仓库物流领域的应用非常普遍,大幅提高了器材仓库物流作业效率,促进了器材仓库管理模式的改进和业务流程优化。目前其主要研究方向之一就是管理信息的可视化。文章正是基于这个目的,采取VR技术、RFID等技术和UML建模设计方法,实现了器材仓库可视化管理信息系统的改造设计,为器材仓库仓库管理水平的提升奠定了基础,也为可视化管理信息系统的设计提供了一定借鉴。

  2. Digital Speed Control System Design for Air Purification Equipment%空气净化装置数字化调速控制系统设计

    Institute of Scientific and Technical Information of China (English)

    石宝松; 张伟; 刘剑; 韩振伟


    地平式激光跟踪发射系统为保证其光路洁净,须进行正压防尘处理。针对外购空气净化设备仅能采用手动风速调节,不能实现计算机远程控制的不足,研发了空气净化设备数字化调速控制系统,以实现对空气净化装置的远程控制与状态监测。本设计采用光电隔离单相交流调压模块实现对风机的驱动,采用霍尔电流传感器实现对风机的电流测量,并采用内部集成A/D转换器和D/A转换器的单片机通过RS422总线接收控制指令,完成对调压模块的输出功率控制和风机电流的实时采集。本系统设计简洁、控制方便、运行可靠、易于维护,实现了设备管理计算机对空气净化设备风速的远程控制。%Horizon emitting laser tracking system to ensure its optical path cleaning, shall be positive dust treatment. For the lack of air purification equipment purchased only with manual speed adjustment and the remote control of a computer can not be achieved, we have developed an air purification equipment digital speed control system to achieve the remote control and status monitoring of air purification device. The design uses a optically isolated single-phase AC voltage regulator module for fan drive,uses a Hall current sensors to achieve the fan current measurement,and uses a microcontroller, which integrates a A/D converter and a D/A converter, to receive the control command via the RS422 bus, to complete the output power control of voltage regulator module, and to realize the real-time collection of fan’ s current. The system is designed to be simple, easy to control, reliable to operate and easy to maintain and realizes the remote control of a device management computer on the wind speed of air purification equipment.

  3. Industrial cogeneration optimization program. Volume II. Appendix A. Conceptual designs and preliminary equipment specifications. Appendix B. Characterization of cogeneration systems (near-term technology). Appendix C. Optimized cogeneration systems

    Energy Technology Data Exchange (ETDEWEB)


    This appendix to a report which evaluates the technical, economic, and institutional aspects of industrial cogeneration for conserving energy in the food, chemical, textile, paper, and petroleum industries contains data, descriptions, and diagrams on conceptual designs and preliminary equipment specifications for cogeneration facilities; characterization of cogeneration systems in terms of fuel utilization, performance, air pollution control, thermal energy storage systems, and capital equipment costs; and optimized cogeneration systems for specific industrial plants. (LCL)

  4. Recovery of Biomolecules from Food Wastes — A Review

    Directory of Open Access Journals (Sweden)

    Antonietta Baiano


    Full Text Available Food wastes are produced by a variety of sources, ranging from agricultural operations to household consumption. About 38% occurs during food processing. At present, the European Union legislation encourages the exploitation of co-products. This valorisation can be achieved through the extraction of high-value components such as proteins, polysaccharides, fibres, flavour compounds, and phytochemicals, which can be re-used as nutritionally and pharmacologically functional ingredients. Extraction can proceed according to solid-liquid extraction, Soxhlet extraction, pressurized fluid extraction, supercritical fluid extraction, ultrasound-assisted extraction, microwave-assisted extraction, pulsed electric field extraction, and enzyme-assisted extraction. Nevertheless, these techniques cannot be used indiscriminately and their choice depends on the type of biomolecules and matrix, the scale processing (laboratory or industrial, the ratio between production costs and economic values of the compounds to be extracted. The vegetable wastes include trimmings, peelings, stems, seeds, shells, bran, residues remaining after extraction of oil, starch, sugar, and juice. The animal-derived wastes include wastes from bred animals, wastes from seafood, wastes from dairy processing. The recovered biomolecules and by-products can be used to produce functional foods or as adjuvants in food processing or in medicinal and pharmaceutical preparations. This work is an overview of the type and amounts of food wastes; food waste legislation; conventional and novel techniques suitable for extracting biomolecules; food, medicinal and pharmaceutical uses of the recovered biomolecules and by-products, and future trends in these areas.

  5. Recovery of biomolecules from food wastes--a review. (United States)

    Baiano, Antonietta


    Food wastes are produced by a variety of sources, ranging from agricultural operations to household consumption. About 38% occurs during food processing. At present, the European Union legislation encourages the exploitation of co-products. This valorisation can be achieved through the extraction of high-value components such as proteins, polysaccharides, fibres, flavour compounds, and phytochemicals, which can be re-used as nutritionally and pharmacologically functional ingredients. Extraction can proceed according to solid-liquid extraction, Soxhlet extraction, pressurized fluid extraction, supercritical fluid extraction, ultrasound-assisted extraction, microwave-assisted extraction, pulsed electric field extraction, and enzyme-assisted extraction. Nevertheless, these techniques cannot be used indiscriminately and their choice depends on the type of biomolecules and matrix, the scale processing (laboratory or industrial), the ratio between production costs and economic values of the compounds to be extracted. The vegetable wastes include trimmings, peelings, stems, seeds, shells, bran, residues remaining after extraction of oil, starch, sugar, and juice. The animal-derived wastes include wastes from bred animals, wastes from seafood, wastes from dairy processing. The recovered biomolecules and by-products can be used to produce functional foods or as adjuvants in food processing or in medicinal and pharmaceutical preparations. This work is an overview of the type and amounts of food wastes; food waste legislation; conventional and novel techniques suitable for extracting biomolecules; food, medicinal and pharmaceutical uses of the recovered biomolecules and by-products, and future trends in these areas.

  6. Silica biomineralization via the self-assembly of helical biomolecules. (United States)

    Liu, Ben; Cao, Yuanyuan; Huang, Zhehao; Duan, Yingying; Che, Shunai


    The biomimetic synthesis of relevant silica materials using biological macromolecules as templates via silica biomineralization processes attract rapidly rising attention toward natural and artificial materials. Biomimetic synthesis studies are useful for improving the understanding of the formation mechanism of the hierarchical structures found in living organisms (such as diatoms and sponges) and for promoting significant developments in the biotechnology, nanotechnology and materials chemistry fields. Chirality is a ubiquitous phenomenon in nature and is an inherent feature of biomolecular components in organisms. Helical biomolecules, one of the most important types of chiral macromolecules, can self-assemble into multiple liquid-crystal structures and be used as biotemplates for silica biomineralization, which renders them particularly useful for fabricating complex silica materials under ambient conditions. Over the past two decades, many new silica materials with hierarchical structures and complex morphologies have been created using helical biomolecules. In this review, the developments in this field are described and the recent progress in silica biomineralization templating using several classes of helical biomolecules, including DNA, polypeptides, cellulose and rod-like viruses is summarized. Particular focus is placed on the formation mechanism of biomolecule-silica materials (BSMs) with hierarchical structures. Finally, current research challenges and future developments are discussed in the conclusion.

  7. Charged porous membrane structures for separation of biomolecules

    NARCIS (Netherlands)

    Kopec, Karina Katarzyna


    Thesis presents various membrane techniques for biomolecules separation. New charged membranes and new methods to introduce charge into the membranes are demonstrated. All chapters present characterization of the hollow fiber membranes produced via the immersion precipitation dry-wet spinning. Apart

  8. Artificial tongue based on metal-biomolecule coordination polymer nanoparticles. (United States)

    Pu, Fang; Ran, Xiang; Ren, Jinsong; Qu, Xiaogang


    We construct an array-based recognition system (the so-called artificial tongue) through the self-assembly of nucleotides, dyes and lanthanide ions. Metal ions are selected as model analytes for verifying its discrimination ability. The work provides valuable insights into the application and development of biomolecule-based materials.

  9. Photochemical Microscale Electrophoresis Allows Fast Quantification of Biomolecule Binding. (United States)

    Möller, Friederike M; Kieß, Michael; Braun, Dieter


    Intricate spatiotemporal patterns emerge when chemical reactions couple to physical transport. We induce electrophoretic transport by a confined photochemical reaction and use it to infer the binding strength of a second, biomolecular binding reaction under physiological conditions. To this end, we use the photoactive compound 2-nitrobenzaldehyde, which releases a proton upon 375 nm irradiation. The charged photoproducts locally perturb electroneutrality due to differential diffusion, giving rise to an electric potential Φ in the 100 μV range on the micrometer scale. Electrophoresis of biomolecules in this field is counterbalanced by back-diffusion within seconds. The biomolecule concentration is measured by fluorescence and settles proportionally to exp(-μ/D Φ). Typically, binding alters either the diffusion coefficient D or the electrophoretic mobility μ. Hence, the local biomolecule fluorescence directly reflects the binding state. A fit to the law of mass action reveals the dissociation constant of the binding reaction. We apply this approach to quantify the binding of the aptamer TBA15 to its protein target human-α-thrombin and to probe the hybridization of DNA. Dissociation constants in the nanomolar regime were determined and match both results in literature and in control experiments using microscale thermophoresis. As our approach is all-optical, isothermal and requires only nanoliter volumes at nanomolar concentrations, it will allow for the fast screening of biomolecule binding in low volume multiwell formats.

  10. Nanofluidic technology for biomolecule applications: a critical review. (United States)

    Napoli, M; Eijkel, J C T; Pennathur, S


    In this review, we present nanofluidic phenomena, particularly as they relate to applications involving analysis of biomolecules within nanofabricated devices. The relevant length scales and physical phenomena that govern biomolecule transport and manipulation within nanofabricated nanofluidic devices are reviewed, the advantages of nanofabricated devices are presented, and relevant applications are cited. Characteristic length scales include the Debye length, the Van der Waals radius, the action distance of hydrogen bonding, the slip length, and macromolecular dimensions. On the basis of the characteristic lengths and related nanofluidic phenomena, a nanofluidic toolbox will be assembled. Nanofluidic phenomena that affect biomolecule behavior within such devices can include ion depletion and enrichment, modified velocity and mobility, permselectivity, steric hindrance, entropy, adsorption, and hydrodynamic interaction. The complex interactions and coupled physics of such phenomena allow for many applications, including biomolecule separation, concentration, reaction/hybridization, sequencing (in the case of DNA) and detection. Examples of devices for such applications will be presented, followed by a discussion of near-term challenges and future thoughts for the field.

  11. Fullerene–biomolecule conjugates and their biomedicinal applications

    Directory of Open Access Journals (Sweden)

    Yang X


    Full Text Available Xinlin Yang,1 Ali Ebrahimi,1 Jie Li,1,2 Quanjun Cui11Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, VA, USA; 2School of Materials Science, Beijing Institute of Technology, Beijing, People's Republic of ChinaAbstract: Fullerenes are among the strongest antioxidants and are characterized as "radical sponges." The research on biomedicinal applications of fullerenes has achieved significant progress since the landmark publication by Friedman et al in 1993. Fullerene–biomolecule conjugates have become an important area of research during the past 2 decades. By a thorough literature search, we attempt to update the information about the synthesis of different types of fullerene–biomolecule conjugates, including fullerene-containing amino acids and peptides, oligonucleotides, sugars, and esters. Moreover, we also discuss in this review recently reported data on the biological and pharmaceutical utilities of these compounds and some other fullerene derivatives of biomedical importance. While within the fullerene–biomolecule conjugates, in which fullerene may act as both an antioxidant and a carrier, specific targeting biomolecules conjugated to fullerene will undoubtedly strengthen the delivery of functional fullerenes to sites of clinical interest.Keywords: fullerene, amino acid, peptide, oligonucleotide, sugar, ester

  12. 玉米剥皮装置的设计研究%Design of the Corn Peeling Equipment

    Institute of Scientific and Technical Information of China (English)

    杨红光; 杨然兵; 尚书旗; 刘立辉; 崔功佩


    According to the requirements of corn peeling device in the agronomic process,this paper introduces the design of corn peeling device which supporting the use of 4YW-2 type corn combine harvester, consisted of a material inlet, peeling device, pressure feeding device, conveying auger, transmission device etc. The peeling device can achieve trans-fer, peeling,corn and bract separated collection. This paper takes the husker rate,seed dropping rate,grain loss rate and productivity as the main index,calculated some parts of structure size. The designed peeling device are compact and co-ordinated in the 4YW-2 type corn harvester, operating smooth and reliable. It can also ensure the peeling rate more than ninety percent, grain broken rate less than four percent,performance index of joint harvest and improve the production ef-ficiency of combine harvester.%根据农艺过程中对玉米剥皮装置的要求,设计了与4 YW-2型玉米联合收获机配套使用的玉米剥皮装置,该部分主要由入料口、剥皮装置、压送装置、输送搅龙及传动装置等部分组成,可以一次作业完成玉米穗的传送、剥皮、玉米与苞叶的分隔收集等作业。为此,以玉米苞叶的剥净率、落籽率、籽粒损失率和生产率为主要指标,计算了部分零部件的结构尺寸。该玉米剥皮装置在4 YW-2型玉米联合收获机上配置紧凑协调,作业顺畅可靠,玉米剥皮过程中剥净率达90%以上、作业损失率低于4%,保证了联合收获的作业性能指标,提高了联合收获机的生产效率。

  13. Discovering the enzyme mimetic activity of metal-organic framework (MOF) for label-free and colorimetric sensing of biomolecules. (United States)

    Wang, Ying; Zhu, Yingjing; Binyam, Atsebeha; Liu, Misha; Wu, Yinan; Li, Fengting


    A label-free sensing strategy based on the enzyme-mimicking activity of MOF was demonstrated for colorimetric detection of biomolecules. Firstly obvious blue color was observed due to the high efficiency of peroxidase-like catalytic activity of Fe-MIL-88A (an ion-based MOF material) toward 3,3',5,5'-tetramethylbenzidine (TMB). Then in the presence of target biomolecule and corresponding aptamer, the mimetic activity of Fe-MIL-88A can be strongly inhibited and used directly to realize the colorimetric detection. On the basis of the interesting findings, we designed a straightforward, label-free and sensitive colorimetric method for biomolecule detection by using the enzyme mimetic property of MOF coupling with molecular recognition element. Compared with the existed publications, our work breaks the routine way by setting up an inorganic-organic MOF-aptamer hybrid platform for colorimetric determination of biomolecules, expanding the targets scope from H2O2 or glucose to biomolecules. As a proof of concept, thrombin and thrombin aptamer was used as a model analyte. The limit of detection of 10nM can be achieved with naked eyes and ultrahigh selectivity of thrombin toward numerous interfering substances with 10-fold concentration was demonstrated significantly. Of note, the method was further applied for the detection of thrombin in human serum samples, showing the results in agreement with those values obtained in an immobilization buffer by the colorimetric method. This inorganic-organic MOF-aptamer sensing strategy may in principle be universally applicable for the detection of a range of environmental or biomedical molecules of interests.

  14. Heterogeneous kinetics of coal gasification. Quarterly technical progress report, 1 May 1982-31 July 1982. [Equipment design

    Energy Technology Data Exchange (ETDEWEB)

    Calo, J.M.


    During the reporting period significant progress was made on two fronts: (1) the steam addition system for transient steam-char kinetic experiments; and (2) the automated data acquisition system for rapid mass programming (control) of the mass spectrometer and data logging. Steam Addition System: Although it is a relatively straightforward matter to produce steam for the reactor, we found that it is quite a challenge to develop a system capable of maintaining a steady and accurately-known flow rate of steam-argon mixtures at high pressure and temperature in alternate flow circuits (i.e., the reactor and purge lines), while simultaneously protecting downstream valving and instrumentation. Thus, the scheme originally proposed has been modified to eliminate potential operating problems associated with the preliminary design. Automated Data Acquisition System: Although the CO/sub 2/ gasification data were obtained manually, the ultimate objective of the project to develop a quantitative understanding of the complete gasification rate process in the complex synthesis gas milieux calls for automated programming (control) of the mass spectrometer. In addition, the current mass spectrometer signal processing technique requires the use of a lock-in amplifier to extract the modulated portion of the total signal due to the beam species only, in an analog mode. In order to allow fast scanning of a number of species, the characteristic dwell time required by the lock-in amplifier on a particular mass peak must be decreased. These requirements are being met by implementing automated mass programming and data collection, and direct digital, phase-sensitive pulse counting.

  15. 装备质量管理体系运行绩效评价指标设计研究%Research on Running Performance Evaluation Index Design of Equipment Quality Management System

    Institute of Scientific and Technical Information of China (English)

    孟冲; 宋华文


    Equipment quality is the basic engineering and life engineering. Equipment quality management system is the ultimate assure of equipment construction scientific development. Equipment quality management running performance level will affect equipment quality directly. In this paper, the basic meanings of equipment quality management running performance are explained, the effect factors of equipment quality management system running are analyzed, concerning of organization factors, dynamic process factors, result factors and extern circumstance factors, the design principles of performance evaluation index are presented, a mixed performance evaluation index system of equipment quality management running is established at last. These will offer important theory bases for estimating equipment quality management ability scientifically and enhancing equipment quality management system construction.%装备质量是装备建设的基础工程和生命工程.装备质量管理体系是推进装备建设科学发展的根本保证,其运行绩效水平的高低,直接影响装备质量.本文界定了装备质量管理体系运行绩效的基本内涵,从组织、动态过程、结果和外部环境等4个方面分析了装备质量管理体系运行的影响因素,提出了绩效评价指标的设计原则,构建了混合型绩效评价指标体系,为科学评价装备质量管理能力,加强和完善装备质量管理体系建设奠定了理论基础.

  16. Nuclear Weapon and Military Equipment

    Directory of Open Access Journals (Sweden)

    N. Natarajan


    Full Text Available Military equipment must survive a, nuclear attack if enough personnel required to operate them remain combat effective. To achieve this goal, the criteria that determine equipment survivability should be established and any new design should incorporate these criteria to evolve a hardened design. An analysis of the overall response by considering the response to each individual nuclear environment can result in a balanced overall hardened system. The paper discusses the criteria required to be known.

  17. A Stochastic Programming Approach with Improved Multi-Criteria Scenario-Based Solution Method for Sustainable Reverse Logistics Design of Waste Electrical and Electronic Equipment (WEEE

    Directory of Open Access Journals (Sweden)

    Hao Yu


    Full Text Available Today, the increased public concern about sustainable development and more stringent environmental regulations have become important driving forces for value recovery from end-of-life and end-of use products through reverse logistics. Waste electrical and electronic equipment (WEEE contains both valuable components that need to be recycled and hazardous substances that have to be properly treated or disposed of, so the design of a reverse logistics system for sustainable treatment of WEEE is of paramount importance. This paper presents a stochastic mixed integer programming model for designing and planning a generic multi-source, multi-echelon, capacitated, and sustainable reverse logistics network for WEEE management under uncertainty. The model takes into account both economic efficiency and environmental impacts in decision-making, and the environmental impacts are evaluated in terms of carbon emissions. A multi-criteria two-stage scenario-based solution method is employed and further developed in this study for generating the optimal solution for the stochastic optimization problem. The proposed model and solution method are validated through a numerical experiment and sensitivity analyses presented later in this paper, and an analysis of the results is also given to provide a deep managerial insight into the application of the proposed stochastic optimization model.

  18. 基于 Raspberry Pi的α能谱测量装置设计%Design of Alpha Spectrum Measurement Equipment Based on Raspberry Pi

    Institute of Scientific and Technical Information of China (English)

    李文涛; 唐泉; 吴淼; 李连山; 崔爽; 龙慧佳; 冯旭


    The paper proposed a design program of αembedded alpha spectrum measurement device based on Raspberry Pi computer.Based on Linux operating system and the Python program, the device realized the re-moteαspectral measurement, real-time display and data storage.Avoiding the operating system migration and driver development in general embedded system development, this design shortens the development period, and the software is compatible with the personal computer and windows operating system.The content includes hard-ware circuit scheme,alpha spectral acquisition board,data transmission and cross platform multithreading spec-trum software design.Tested through the Am -241 source, the repeatability and stability of the measurement equipment meet the requirements.%提出了一种基于Raspberry Pi计算机的嵌入式α能谱测量装置的设计方案,基于Linux操作系统和Python语言编写程序,并利用无线网络远程控制,实现了远程α能谱测量、实时显示和数据保存等功能,软件设计过程避免了操作系统迁移、驱动开发等工作,具有开发周期短,通用于PC机的特点。内容包括α能谱仪的硬件电路、数据传输和跨平台多线程能谱软件的设计。使用241 Am源对测量系统主要性能进行了测试,重复性及稳定性符合要求。

  19. Interactions between cationic liposomes and drugs or biomolecules

    Directory of Open Access Journals (Sweden)



    Full Text Available Multiple uses for synthetic cationic liposomes composed of dioctadecyldimethylammonium bromide (DODAB bilayer vesicles are presented. Drugs or biomolecules can be solubilized or incorporated in the cationic bilayers. The cationic liposomes themselves can act as antimicrobial agents causing death of bacteria and fungi at concentrations that barely affect mammalian cells in culture. Silica particles or polystyrene microspheres can be functionalized by coverage with DODAB bilayers or phospholipid monolayers. Negatively charged antigenic proteins can be carried by the cationic liposomes which generate a remarkable immunoadjuvant action. Nucleotides or DNA can be physically adsorbed to the cationic liposomes to be transferred to mammalian cells for gene therapy. An overview of the interactions between DODAB vesicles and some biomolecules or drugs clearly points out their versatility for useful applications in a near future.

  20. Interactions between cationic liposomes and drugs or biomolecules. (United States)

    Carmona-Ribeiro, A M


    Multiple uses for synthetic cationic liposomes composed of dioctadecyldimethylammonium bromide (DODAB) bilayer vesicles are presented. Drugs or biomolecules can be solubilized or incorporated in the cationic bilayers. The cationic liposomes themselves can act as antimicrobial agents causing death of bacteria and fungi at concentrations that barely affect mammalian cells in culture. Silica particles or polystyrene microspheres can be functionalized by coverage with DODAB bilayers or phospholipid monolayers. Negatively charged antigenic proteins can be carried by the cationic liposomes which generate a remarkable immunoadjuvant action. Nucleotides or DNA can be physically adsorbed to the cationic liposomes to be transferred to mammalian cells for gene therapy. An overview of the interactions between DODAB vesicles and some biomolecules or drugs clearly points out their versatility for useful applications in a near future.

  1. Biomolecule-nanoparticle hybrids as functional units for nanobiotechnology. (United States)

    Baron, Ronan; Willner, Bilha; Willner, Itamar


    Biomolecule-metal or semiconductor nanoparticle (NP) hybrid systems combine the recognition and catalytic properties of biomolecules with the unique electronic and optical properties of NPs. This enables the application of the hybrid systems in developing new electronic and optical biosensors, to synthesize nanowires and nanocircuits, and to fabricate new devices. Metal NPs are employed as nano-connectors that activate redox enzymes, and they act as electrical or optical labels for biorecognition events. Similarly, semiconductor NPs act as optical probes for biorecognition processes. Double-stranded DNA or protein chains that are modified with metallic nanoclusters act as templates for the synthesis of metallic nanowires. The nanowires are used as building blocks to assemble nano-devices such as a transistor or a nanotransporter.

  2. Surface Treatment of Polymeric Materials Controlling the Adhesion of Biomolecules

    Directory of Open Access Journals (Sweden)

    Willy Zorzi


    Full Text Available This review describes different strategies of surface elaboration for a better control of biomolecule adsorption. After a brief description of the fundamental interactions between surfaces and biomolecules, various routes of surface elaboration are presented dealing with the attachment of functional groups mostly thanks to plasma techniques, with the grafting to and from methods, and with the adsorption of surfactants. The grafting of stimuli-responsive polymers is also pointed out. Then, the discussion is focused on the protein adsorption phenomena showing how their interactions with solid surfaces are complex. The adsorption mechanism is proved to be dependent on the solid surface physicochemical properties as well as on the surface and conformation properties of the proteins. Different behaviors are also reported for complex multiple protein solutions.

  3. Optical Sensors for Biomolecules Using Nanoporous Sol-Gel Materials (United States)

    Fang, Jonathan; Zhou, Jing C.; Lan, Esther H.; Dunn, Bruce; Gillman, Patricia L.; Smith, Scott M.


    An important consideration for space missions to Mars is the ability to detect biosignatures. Solid-state sensing elements for optical detection of biological entities are possible using sol-gel based biologically active materials. We have used these materials as optical sensing elements in a variety of bioassays, including immunoassays and enzyme assays. By immobilizing an appropriate biomolecule in the sol-gel sensing element, we have successfully detected analytes such as amino acids and hormones. In the case of the amino acid glutamate, the enzyme glutamate dehydrogenase was the immobilized molecule, whereas in the case of the hormone cortisol, an anti-cortisol antibody was immobilized in the sensing element. In this previous work with immobilized enzymes and antibodies, excellent sensitivity and specificity were demonstrated in a variety of formats including bulk materials, thin films and fibers. We believe that the sol-gel approach is an attractive platform for bioastronautics sensing applications because of the ability to detect a wide range of entities such as amino acids, fatty acids, hopanes, porphyrins, etc. The sol-gel approach produces an optically transparent 3D silica matrix that forms around the biomolecule of interest, thus stabilizing its structure and functionality while allowing for optical detection. This encapsulation process protects the biomolecule and leads to a more "rugged" sensor. The nanoporous structure of the sol-gel matrix allows diffusion of small target molecules but keeps larger, biomolecules immobilized in the pores. We are currently developing these biologically active sol-gel materials into small portable devices for on-orbit cortisol detection

  4. The first search for glycine and other biomolecules

    CERN Document Server

    Storey, J W V


    In the 1970s the microwave spectroscopy group at Monash University became the first in the world to determine the spectral frequencies of urea, glycine, and several other biomolecules. We immediately searched for these at Parkes, using existing centimetre-wave receivers plus newly built receivers that operated at frequencies as high as 75GHz (and used just the central 17 m of the dish). Although these searches were largely unsuccessful, they helped launch the now flourishing field of astrobiology.

  5. Models and algorithms for biomolecules and molecular networks

    CERN Document Server

    DasGupta, Bhaskar


    By providing expositions to modeling principles, theories, computational solutions, and open problems, this reference presents a full scope on relevant biological phenomena, modeling frameworks, technical challenges, and algorithms. * Up-to-date developments of structures of biomolecules, systems biology, advanced models, and algorithms * Sampling techniques for estimating evolutionary rates and generating molecular structures * Accurate computation of probability landscape of stochastic networks, solving discrete chemical master equations * End-of-chapter exercises

  6. Transition-Metal-Free Biomolecule-Based Flexible Asymmetric Supercapacitors. (United States)

    Yang, Yun; Wang, Hua; Hao, Rui; Guo, Lin


    A transition-metal-free asymmetric supercapacitor (ASC) is successfully fabricated based on an earth-abundant biomass derived redox-active biomolecule, named lawsone. Such an ASC exhibits comparable or even higher energy densities than most of the recently reported transition-metal-based ASCs, and this green ASC generation from renewable resources is promising for addressing current issues of electronic hazard processing, high cost, and unsustainability.

  7. Renewable-Biomolecule-Based Full Lithium-Ion Batteries. (United States)

    Hu, Pengfei; Wang, Hua; Yang, Yun; Yang, Jie; Lin, Jie; Guo, Lin


    A renewable-biomolecule-based full lithium-ion battery is successfully fabricated for the first time. Naturally derivable emodin and humic acid based electrodes are used as cathode and anode, respectively. The as-assembled batteries exhibit superb specific capacity and substantial operating voltage capable of powering a wearable electronic watch, suggesting the great potential for practical applications with the significant merits of sustainability and biocompatibility.

  8. In situ hybridization of superparamagnetic iron-biomolecule nanoparticles. (United States)

    Moghimi, Nafiseh; Donkor, Apraku David; Mohapatra, Mamata; Thomas, Joseph Palathinkal; Su, Zhengding; Tang, Xiaowu Shirley; Leung, Kam Tong


    The increase in interest in the integration of organic-inorganic nanostructures in recent years has promoted the use of hybrid nanoparticles (HNPs) in medicine, energy conversion, and other applications. Conventional hybridization methods are, however, often long, complicated, and multistepped, and they involve biomolecules and discrete nanostructures as separate entities, all of which hinder the practical use of the resulting HNPs. Here, we present a novel, in situ approach to synthesizing size-specific HNPs using Fe-biomolecule complexes as the building blocks. We choose an anticancer peptide (p53p, MW 1.8 kDa) and an enzyme (GOx, MW 160 kDa) as model molecules to demonstrate the versatility of the method toward different types of molecules over a large size range. We show that electrostatic interaction for complex formation of metal hydroxide ion with the partially charged side of biomolecule in the solution is the key to hybridization of metal-biomolecule materials. Electrochemical deposition is then used to produce hybrid NPs from these complexes. These HNPs with controllable sizes ranging from 30 nm to 3.5 μm are found to exhibit superparamagnetic behavior, which is a big challenge for particles in this size regime. As an example of greatly improved properties and functionality of the new hybrid material, in vitro toxicity assessment of Fe-GOx HNPs shows no adverse effect, and the Fe-p53p HNPs are found to selectively bind to cancer cells. The superparamagnetic nature of these HNPs (superparamagnetic even above the size regime of 15-20 nm!), their biocompatibility, and the direct integration approach are fundamentally important to biomineralization and general synthesis strategy for bioinspired functional materials.

  9. Magnetic-based biomolecule detection using giant magnetoresistance sensors (United States)

    Kokkinis, G.; Jamalieh, M.; Cardoso, F.; Cardoso, S.; Keplinger, F.; Giouroudi, I.


    This paper presents a novel microfluidic chip for in-vitro detection of biomolecules tagged by magnetic microparticles (MAPs) suspended in a static fluid. The system consists of two microfluidic channels: a reference channel in which bare MAPs are suspended and a detection channel in which magnetically tagged biomolecules are suspended (LMAPs). The LMAPs are functionalized MAPs (of the same magnetic volume as the ones in the reference channel) with attached biomolecules. The overall, non-magnetic volume of the LMAPs is greater than that of the bare MAPs. Current carrying microconductors are positioned underneath the channels in order to impose a magnetic field gradient to the MAPs and LMAPs and move them from the inlet to the outlet of the channels without flow. The innovative aspect of the proposed method is that the induced velocity on the MAPs and LMAPs, while imposed to the same magnetic field gradient, is inversely proportional to their overall, non-magnetic volume. This is due to the enhanced Stokes drag force exerted on the LMAPs, resulting from the greater volume and altered hydrodynamic shape. This induced velocity is measured by utilizing Giant Magnetoresistance (GMR) sensor pairs fabricated underneath the first and the last microconductors. Detected differences in velocity between the LMAPs and the reference MAPs indicate the presence of biomolecules in the static liquid sample. We also present a novel method for signal acquisition and demodulation: expensive function generators, data acquisition devices, and lock-in amplifiers were substituted by a generic PC sound card and an algorithm combining the Fast Fourier Transform of the signal with a peak detection routine. Experiments with functionalized MAPs and magnetically tagged Escherichia coli (representing the LMAPs) were carried out as a proof of concept. In order to identify the detection limit of the GMR sensor, single MAP (2.8 μm diameter) detection was performed.

  10. Creating "living" polymer surfaces to pattern biomolecules and cells on common plastics. (United States)

    Li, Chunyan; Glidle, Andrew; Yuan, Xiaofei; Hu, Zhixiong; Pulleine, Ellie; Cooper, Jon; Yang, Wantai; Yin, Huabing


    Creating patterns of biomolecules and cells has been applied widely in many fields associated with the life sciences, including diagnostics. In these applications it has become increasingly apparent that the spatiotemporal arrangement of biological molecules in vitro is important for the investigation of the cellular functions found in vivo. However, the cell patterning techniques often used are limited to creating 2D functional surfaces on glass and silicon. In addition, in general, these procedures are not easy to implement in conventional biological laboratories. Here, we show the formation of a living poly(ethylene glycol) (PEG) layer that can be patterned with visible light on plastic surfaces. This new and simple method can be expanded to pattern multiple types of biomolecule on either a previously formed PEG layer or a plastic substrate. Using common plastic wares (i.e., polyethylene films and polystyrene cell culture Petri-dishes), we demonstrate that these PEG-modified surfaces have a high resistance to protein adsorption and cell adhesion, while at the same time, being capable of undergoing further molecular grafting with bioactive motifs. With a photomask and a fluid delivery system, we illustrate a flexible way to immobilize biological functions with a high degree of 2D and 3D spatial control. We anticipate that our method can be easily implemented in a typical life science laboratory (without the need for specialized lithography equipment) offering the prospect of imparting desirable properties to plastic products, for example, the creation of functional microenvironments in biological studies or reducing biological adhesion to surfaces.

  11. Equipment Obsolescence Management Program

    Energy Technology Data Exchange (ETDEWEB)

    Redmond, J.


    Nuclear Power Plant (NPP) Operators are challenged with securing reliable supply channels for safety related equipment due to equipment obsolescence. Many Original Equipment Manufacturers (OEMs) have terminated production of spare parts and product life-cycle support. The average component life cycles are much shorter than the NPP design life, which means that replacement components and parts for the original NPP systems are not available for the complete design life of the NPPs. The lack or scarcity of replacement parts adversely affects plant reliability and ultimately the profitability of the affected NPPs. This problem is further compounded when NPPs pursue license renewal and approval for plant-life extension. A reliable and predictable supply of replacement co components is necessary for NPPs to remain economically competitive and meet regulatory requirements and guidelines. Electrical and I and C components, in particular, have short product life cycles and obsolescence issues must be managed pro actively and not reactively in order to mitigate the risk to the NPP to ensure reliable and economic NPP operation. (Author)

  12. The nano revolution: bottom-up manufacturing with biomolecules (United States)

    Li, Yi-Fen; Li, Jing; Paavola, Chad; Kagawa, Hiromi; Chan, Suzanne L.; Trent, Jonathan D.


    As the nano-scale becomes a focus for engineering electronic, photonic, medical, and other important devices, an unprecedented role for biomolecules is emerging to address one of the most formidable problems in nano-manufacturing: precise manipulation and organization of matter on the nano-scale. Biomolecules are a solution to this problem because they themselves are nanoscale particles with intrinsic properties that allow them to precisely self-assemble and self-organize into the amazing diversity of structures observed in nature. Indeed, there is ample evidence that the combination of molecular recognition and self-assembly combined with mutation, selection, and replication have the potential to create structures that could truly revolutionize manufacturing processes in many sectors of industry. Genetically engineered biomolecules are already being used to make the next generation of nano-scale templates, nano-detailed masks, and molecular scaffolds for the future manufacturing of electronic devices, medical diagnostic tools, and chemical engineering interfaces. Here we present an example of this type of technology by showing how a protein can be genetically modified to form a new structure and coated with metal to lead the way to producing "nano-wires," which may ultimately become the basis for self-assembled circuitry.

  13. Applications of polymers for biomolecule immobilization in electrochemical biosensors

    Energy Technology Data Exchange (ETDEWEB)

    Teles, F.R.R. [IBB - Instituto de Biotecnologia e Bioengenharia, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Avenida Rovisco Pais, 1049-001 Lisboa (Portugal); Fonseca, L.P. [IBB - Instituto de Biotecnologia e Bioengenharia, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Avenida Rovisco Pais, 1049-001 Lisboa (Portugal)], E-mail:


    Polymers are becoming inseparable from biomolecule immobilization strategies and biosensor platforms. Their original role as electrical insulators has been progressively substituted by their electrical conductive abilities, which opens a new and broad scope of applications. In addition, recent advances in diagnostic chips and microfluidic systems, together with the requirements of mass-production technologies, have raised the need to replace glass by polymeric materials, which are more suitable for production through simple manufacturing processes. Conducting polymers (CPs), in particular, are especially amenable for electrochemical biosensor development for providing biomolecule immobilization and for rapid electron transfer. It is expected that the combination of known polymer substrates, but also new transducing and biocompatible interfaces, with nanobiotechnological structures, like nanoparticles, carbon nanotubes (CNTs) and nanoengineered 'smart' polymers, may generate composites with new and interesting properties, providing higher sensitivity and stability of the immobilized molecules, thus constituting the basis for new and improved analytical devices for biomedical and other applications. This review covers the state-of-the-art and main novelties about the use of polymers for immobilization of biomolecules in electrochemical biosensor platforms.

  14. Repulsive effects of hydrophobic diamond thin films on biomolecule detection

    Energy Technology Data Exchange (ETDEWEB)

    Ruslinda, A. Rahim, E-mail: [Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Jln Kgr-Alor Setar, Seriab, 01000 Kangar, Perlis (Malaysia); Department of Nano Science and Nano Engineering, School of Advance Science and Engineering, Ohkubo 3-4-1, Shinjuku, 169-8555 Tokyo (Japan); Ishiyama, Y. [Department of Nano Science and Nano Engineering, School of Advance Science and Engineering, Ohkubo 3-4-1, Shinjuku, 169-8555 Tokyo (Japan); Penmatsa, V. [Department of Mechanical and Materials Engineering, Florida International University, 10555 West Flagler Street, Miami, FL 33174 (United States); Ibori, S.; Kawarada, H. [Department of Nano Science and Nano Engineering, School of Advance Science and Engineering, Ohkubo 3-4-1, Shinjuku, 169-8555 Tokyo (Japan)


    Highlights: • We report the effect of fluorine plasma treatment on diamond thin film to resist the nonspecific adsorption of biomolecules. • The diamond thin film were highly hydrophobic with a surface energy value of ∼25 mN/m. • The repulsive effect shows excellent binding efficiency for both DNA and HIV-1 Tat protein. - Abstract: The repulsive effect of hydrophobic diamond thin film on biomolecule detection, such as single-nucleotide polymorphisms and human immunodeficiency virus type 1 trans-activator of transcription peptide protein detection, was investigated using a mixture of a fluorine-, amine-, and hydrogen-terminated diamond surfaces. These chemical modifications lead to the formation of a surface that effectively resists the nonspecific adsorption of proteins and other biomolecules. The effect of fluorine plasma treatment on elemental composition was also investigated via X-ray photoelectron spectroscopy (XPS). XPS results revealed a fluorocarbon layer on the diamond thin films. The contact angle measurement results indicated that the fluorine-treated diamond thin films were highly hydrophobic with a surface energy value of ∼25 mN/m.

  15. 46 CFR 154.1005 - Equipment approval. (United States)


    ... carried. (b) Each submerged cargo pump motor installation must be specially approved by the Commandant (CG... FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Electrical § 154.1005 Equipment approval. (a) Electrical equipment that is required to be intrinsically safe...

  16. Proceedings of FED remote maintenance equipment workshop

    Energy Technology Data Exchange (ETDEWEB)

    Sager, P.; Garin, J.; Hager, E.R.; Spampinato, P.T.; Tobias, D.; Young, N.


    A workshop was convened in two sessions in January and March 1981, on the remote maintenance equipment for the Fusion Engineering Device (FED). The objectives of the first session were to familiarize the participants with the status of the design of the FED and to develop a remote maintenance equipment list for the FED. The objective of the second session was to have the participants present design concepts for the equipment which had been identified in the first session. The equipment list was developed for general purpose and special purpose equipment. The general purpose equipment was categorized as manipulators and other, while the special purpose equipment was subdivided according to the reactor subsystem it serviced: electrical, magnetic, and nuclear. Both mobile and fixed base manipulators were identified. Handling machines were identified as the major requirement for special purpose equipment.

  17. 多智能体的设备采购合同管理系统建模与设计%Using Swarm Model to Analyze and Design Multi-Agent Based Equipment Purchasing Contract Management System

    Institute of Scientific and Technical Information of China (English)

    王玮; 徐一帆


    从现代设备采购合同管理的概念出发,首先介绍了设备采购合同管理所面临的新的挑战,然后提出了基于多智能体的设备采购合同管理系统模型.多智能体系统和设备采购合同管理系统都是非常复杂的.为了降低开发基于多智能体设备采购合同管理系统的难度,采用SWARM建模方法,分析和设计了一个简化的设备采购合同管理系统.%Based on the concept of modern equipment purchasing contract management, we first introduce the new challenge to equipment purchasing contract management, and then present a new equipment purchasing contract management framework based on multi-agent theory. Since multi-agent system and equipment purchasing contract management system are both very complex, we use swarm model to analyze and design a simplified equipment purchasing contract management system in order to reduce the difficulty of developing the multi-agent based equipment purchasing contract management system.

  18. Disposable pen-shaped capillary gel electrophoresis cartridge for fluorescence detection of bio-molecules (United States)

    Amirkhanian, Varoujan; Tsai, Shou-Kuan


    We introduce a novel and cost-effective capillary gel electrophoresis (CGE) system utilizing disposable pen-shaped gelcartridges for highly efficient, high speed, high throughput fluorescence detection of bio-molecules. The CGE system has been integrated with dual excitation and emission optical-fibers with micro-ball end design for fluorescence detection of bio-molecules separated and detected in a disposable pen-shaped capillary gel electrophoresis cartridge. The high-performance capillary gel electrophoresis (CGE) analyzer has been optimized for glycoprotein analysis type applications. Using commercially available labeling agent such as ANTS (8-aminonapthalene-1,3,6- trisulfonate) as an indicator, the capillary gel electrophoresis-based glycan analyzer provides high detection sensitivity and high resolving power in 2-5 minutes of separations. The system can hold total of 96 samples, which can be automatically analyzed within 4-5 hours. This affordable fiber optic based fluorescence detection system provides fast run times (4 minutes vs. 20 minutes with other CE systems), provides improved peak resolution, good linear dynamic range and reproducible migration times, that can be used in laboratories for high speed glycan (N-glycan) profiling applications. The CGE-based glycan analyzer will significantly increase the pace at which glycoprotein research is performed in the labs, saving hours of preparation time and assuring accurate, consistent and economical results.

  19. Helium Atmosphere Chamber for Soft X-ray Spectroscopy of Biomolecules (United States)

    Carpenter, Matthew Hollis

    X-ray Absorption Spectroscopy (XAS) is a powerful technique that can provide important information for the study of biological systems. The study of the structure and chemistry at the active metal sites in metalloproteins is important not only for the understanding of the function of the molecules themselves, but also in the hope that the mechanisms once understood may lead to advancement in catalytic chemistry and materials. While techniques using high-energy "hard" X-rays have been well developed for over three decades, the highly useful low-energy or "soft" X-ray regime has seen more recent application to biomolecules. Furthermore, the technical necessities of soft X-ray experiments - most notably the need for Ultra-High Vacuum (UHV) - place restrictions on the form and state of the sample and usually preclude liquid or frozen solution measurements. The Helium atmosphere chamber is designed to allow soft XAS of biomolecules and model compounds in a frozen state without exposure to UHV by isolating the sample inside a cryogenic gaseous exchange gas environment. The implementation allows easier sample handling than typical UHV chambers and measurement in conditions ranging from atmosphere (760 Torr) to 100s of mTorr. The system integrates with the existing end station setup at beam line 4.0.2 at the Advanced Light Source at Lawrence Berkeley National Laboratory.

  20. Aircraft Wiring Support Equipment Integration Laboratory (AWSEIL) (United States)

    Federal Laboratory Consortium — Purpose:The Aircraft Wiring Support Equipment Integration Laboratory (AWSEIL) provides a variety of research, design engineering and prototype fabrication services...

  1. Aircraft Wiring Support Equipment Integration Laboratory (AWSEIL) (United States)

    Federal Laboratory Consortium — Purpose: The Aircraft Wiring Support Equipment Integration Laboratory (AWSEIL) provides a variety of research, design engineering and prototype fabrication services...

  2. 民政专用纳米光催化空气净化消毒机的设计与实现%Design and implementation of civil administration dedicated nano-TiO2 photocatalytic air antiseptic equipment

    Institute of Scientific and Technical Information of China (English)

    李秉杰; 王志良; 李伯森; 张培昆; 刘冀伟; 孟浩


    In order to reduce the air pollution discharged by funeral parlor of civil administration section during the funeral processes ,special antiseptic equipment was developed to purify the in-door air .Based on the filter technology ,activated carbon adsorption technology and photo-cata-lytic technology ,an intellectualized air flow control system was designed applying the modified chaos algorithm and PID optimal control algorithm .Then the prototype equipment was built at the optimal parameters .As shown by the experimental results ,the designed nano-TiO2 photo-catalytic antiseptic equipment is easy to operate and has a powerful antiseptic ability .This equip-ment can satisfyingly disinfect the air ,and therefore can replace the ultraviolet antiseptic equip-ment in the funeral parlor .%  为解决民政行业殡仪场所空气污染问题,研制了一种专用于殡仪场所空气净化的消毒机。以滤网过滤技术、活性炭吸附技术、纳米光催化技术为设计基础,应用改进混沌优化算法与智能PID 最优控制算法设计了智能风面控制系统,优化设计了空气消毒机的结构参数,最后制作样机。结果表明,设计的纳米TiO2光催化空气净化消毒机具有良好的消毒能力且操作简便,对环境空气具有良好的消毒净化功能,可替代紫外线用于殡仪场所空气净化和消毒。


    Energy Technology Data Exchange (ETDEWEB)



    The objective and the scope of this document are to list and briefly describe the major mobile equipment necessary for waste package (WP) Transport and Emplacement in the proposed subsurface nuclear waste repository at Yucca Mountain. Primary performance characteristics and some specialized design features of the equipment are explained and summarized in the individual subsections of this document. The Transport and Emplacement equipment described in this document consists of the following: (1) WP Transporter; (2) Reusable Rail Car; (3) Emplacement Gantry; (4) Gantry Carrier; and (5) Transport Locomotive.

  4. Equipment for gas conversion

    Energy Technology Data Exchange (ETDEWEB)

    Tabata, K.; Matsumoto, I.


    Equipment is proposed for vapor conversion of hydrocarbons (Uv), possibly in a mixture with air, in order to produce an inorganic gas, which chiefly consists of H2 and COx. It consists of a reaction pipe made of an inorganic refractory ceramic and equipped along the wall circumference with heaters. The reaction pipe is filled with a combined, multilayer catalyst (Kt) carrier, made of gamma-A1203 which in the transverse cross section has a multipore reticular or fibrous structure. Replacement of the traditional steel (St) materials for the walls of the reaction pipe with ceramic materials reduces the output of the hydrocarbon which contaminates the surface (Pv) of the catalyst; the use of a multilayer carrier for the catalyst made of gamma-A1203 with a porous reticular or fibrous structure reduces the pressure losses in the reactor and facilitates the replacement of the spent catalyst. The equipment is designed for vapor conversion of natural gas, C3H8, and vapors of kerosene, naphtha and so on.

  5. 装备采购仿真系统总体设计研究%Research on Overall Design of Equipment Acquisition Simulation System

    Institute of Scientific and Technical Information of China (English)



    针对我军装备采购仿真系统建设过程中总体规划不足,技术标准不清的现实问题,按照软件工程化管理的逻辑思维,在全面分析系统建设需求的基础上,提出系统基本功能布局和总体设计方案;明确了系统在需求分析、立项论证和研制3个主要采购阶段的功能定位;提出了基于 HLA 基本思想的4层体系结构,支持系统实现仿真环境下的需求论证与评估、技术成熟度评估和项目核心要素管理等主要装备采购活动。%Aiming at existing problems during construction of our military equipment acquisition simulation such as inadequate overall planning and unclear technical standards,according to the logics of software engineering management,based on requirements analysis of system construction,the pa‐per puts forward basic functional layout and general design scheme of the system,make clear func‐tional layout of system in three main acquisition phases:requirement analysis,project demonstration and development and puts forward a HLA‐based four‐layer architectural structure to support main e‐quipment acquisition activities including demonstration and evaluation of system requirements,evalu‐ation on technology maturity and core elements management of project in a simulation environment.

  6. 太阳能温室装备化的构想与新设计%The idea of solar greenhouse equipment and new design

    Institute of Scientific and Technical Information of China (English)

    文麒麟; 石能文; 郑宏飞; 文鹏


      温室是解决农产品跨季节生长和地域性紧缺的有效途径,在温室中配置太阳能集热和储能单元构成储能型太阳能温室可以改善作物的微气候,同时可以保证作物在高寒地区寒冷季节正常生长。文章系统综述了储能型太阳能温室技术的发展,介绍了近几年温室技术与太阳能技术的结合情况,分析储能型太阳能温室技术的发展趋势,提出储能型太阳能温室装备化的构想,给出了一个新设计结构,为太阳能在农业生产上的利用提供借鉴。%  The greenhouse is an effective way to solve inter-seasonal growth of agricultural products and regional shortage. Configuration of solar collectors and energy storage unit storage-type solar greenhouse can improve the microclimate of the crop in the greenhouse, at the same time, can guarantee the normal growth of crops in the cold season and the cold region. In the article,development of storage type solar greenhouse technology was systematically summarized, the combination of the recent years' greenhouse technology and solar energy technology were introduced, the development trend of energy storage type solar greenhouse technology was analyzed, the idea of the equipment of energy storage type solar greenhouse was put forward, and a new design structure was gave, so reference was provided for agricultural production use.

  7. 火炮驻退杆变深度沟槽测量专机设计与应用%Design and applications of the special detection equipment for variable depth groove in cannon recoil rod

    Institute of Scientific and Technical Information of China (English)

    谭胜龙; 龚运环


    针对火炮驻退杆变深度沟槽加工后人工手动检测精度低,效率低,影响火炮生产线生产效率的问题,结合某火炮驻退杆生产线设备火炮驻退杆拉槽机,设计了用于驻退杆变深度沟槽测量专机,实现自动化测量.%Aiming at the low precision and low efficiency of the manual measurement for the variable depth of groove in the cannon recoil rod, and considering the manual measurement affecting the cannon production efficiency. To link up with the equipments of cannon recoil rod production line, a special detection equipment for variable depth groove in cannon recoil rod is designed, and this equipment runs automatically.

  8. 基于RFID的电力通信设备管理系统的设计与应用%Design and Application of Management System of Power Communication Equipment Based on RFID Technology

    Institute of Scientific and Technical Information of China (English)

    王阳; 张正文


      针对目前电力通信设备仓库管理中存在的问题,设计应用基于RFID的B/S模式通信设备管理系统,实现对电力通信设备的动态管理,节约人力和物资成本,提高精益集约化管理水平。%Power communications equipment for the current problems in warehouse management was discussed. Designing RFID-based applications B/S mode communication device management system, automatically collect information communication equipment, to achieve the dynamic management of power communication equipment, saving manpower and material costs, increase lean intensive management level.

  9. Several Problems Needing Attention in Process Design and Equipment Selection in WWTP%城镇污水厂工艺设计和设备选型应注意的问题

    Institute of Scientific and Technical Information of China (English)



    对几种采用不同工艺污水处理厂的工艺设计和设备选型进行了分析,结合污水处理厂运行的实际情况,总结了工艺设计和设备选型应注意的几个问题,同时对设计进行了优化.%The process design and equipment selection in WWTPs using different types of processes were analyzed. Combined with the actual operation in WWTP, several problems needing attention in process design and equipment selection in WWTP were summarized, and the design was optimized.

  10. 浅述电视综艺演播室灯光设备层的结构和设计%Discussion on Structure and Design of Lighting Equipment Layer in TV Studio

    Institute of Scientific and Technical Information of China (English)



    In this paper, the author introduced the structure and design ideas of the lighting equipment layer of TV studio, and elaborated that the lighting equipment should not be simply attributable to the scope of the building structure, but be designed by lighting technicians, and lighting should be highlighted in the design, taking into account other professional.%介绍电视综艺演播室灯光设备层的结构和设计思路,阐述灯光设备层不应简单归属建筑结构范畴,应由灯光技术人员进行设计,设计中应突出灯光专业、兼顾其他从属专业。

  11. Quantum Simulations of Solvated Biomolecules Using Hybrid Methods (United States)

    Hodak, Miroslav


    One of the most important challenges in quantum simulations on biomolecules is efficient and accurate inclusion of the solvent, because the solvent atoms usually outnumber those in the biomolecule of interest. We have developed a hybrid method that allows for explicit quantum-mechanical treatment of the solvent at low computational cost. In this method, Kohn-Sham (KS) density functional theory (DFT) is combined with an orbital-free (OF) DFT. Kohn-Sham (KS) DFT is used to describe the biomolecule and its first solvation shells, while the orbital-free (OF) DFT is employed for the rest of the solvent. The OF part is fully O(N) and capable of handling 10^5 solvent molecules on current parallel supercomputers, while taking only ˜ 10 % of the total time. The compatibility between the KS and OF DFT methods enables seamless integration between the two. In particular, the flow of solvent molecules across the KS/OF interface is allowed and the total energy is conserved. As the first large-scale applications, the hybrid method has been used to investigate the binding of copper ions to proteins involved in prion (PrP) and Parkinson's diseases. Our results for the PrP, which causes mad cow disease when misfolded, resolve a contradiction found in experiments, in which a stronger binding mode is replaced by a weaker one when concentration of copper ions is increased, and show how it can act as a copper buffer. Furthermore, incorporation of copper stabilizes the structure of the full-length PrP, suggesting its protective role in prion diseases. For alpha-synuclein, a Parkinson's disease (PD) protein, we show that Cu binding modifies the protein structurally, making it more susceptible to misfolding -- an initial step in the onset of PD. In collaboration with W. Lu, F. Rose and J. Bernholc.

  12. Specific and quantitative labeling of biomolecules using click chemistry. (United States)

    Horisawa, Kenichi


    Specific and highly efficient fluorescent labeling techniques for biomolecules, especially for proteins, are required for the quantitative analyses of bio-phenomena and for subsequent systems biology. Although expression of exogenous proteins fused with fluorescent tags, such as green fluorescent protein, is the most widely used method for quantitative bio-analysis, the following problems need to be considered carefully: (1) precise stoichiometric control in living cells is difficult, and (2) the bulkiness of the fluorescent tags restricts analysis of the inherent physical and biological properties of the proteins. Therefore, novel techniques to specifically and stoichiometrically label intrinsic proteins or other biomolecules in living cells should be developed. Click chemistry reactions (e.g., Huisgen cycloaddition and Staudinger ligation) are the most promising approaches for this purpose, because these chemical reactions have following advantages: (1) bioorthogonal reactions; (2) mild reaction conditions suitable for fragile biomolecules, cells, and tissues; (3) extremely high reaction ratio; (4) small size of the functional groups for the cross-coupling reactions; (5) stable covalent bonding; and (6) simple metabolic labeling procedures in living cells, using various biomolecular analogs. Diverse quantitative biological studies have been carried out using this technology (e.g., quantification of novel synthesized proteins and observation of post-translational modifications). In this review, I explain the basics of chemical probing with click chemistry, and discuss its recent applications in the field of quantitative biology. Furthermore, I discuss the capability, significance, and future of the chemical probing of proteins, with an emphasis on the use of click chemistry in the field of the quantitative biology.

  13. Specific and quantitative labeling of biomolecules using click chemistry

    Directory of Open Access Journals (Sweden)

    Kenichi eHorisawa


    Full Text Available Specific and highly efficient fluorescent labeling techniques for biomolecules, especially for proteins, are required for the quantitative analyses of bio-phenomena and for subsequent systems biology. Although expression of exogenous proteins fused with fluorescent tags, such as green fluorescent protein, is the most widely used method for quantitative bio-analysis, the following problems need to be considered carefully: 1 precise stoichiometric control in living cells is difficult, and 2 the bulkiness of the fluorescent tags restricts analysis of the inherent physical and biological properties of the proteins. Therefore, novel techniques to specifically and stoichiometrically label intrinsic proteins or other biomolecules in living cells should be developed. Click chemistry reactions (e.g., Huisgen cycloaddition and Staudinger ligation are the most promising approaches for this purpose, because these chemical reactions have following advantages: 1 bioorthogonal reactions; 2 mild reaction conditions suitable for fragile biomolecules, cells, and tissues; 3 extremely high reaction ratio; 4 small size of the functional groups for the cross-coupling reactions; 5 stable covalent bonding; and 6 simple metabolic labeling procedures in living cells, using various biomolecular analogs. Diverse quantitative biological studies have been carried out using this technology (e.g., quantification of novel synthesized proteins and observation of post-translational modifications. In this review, I explain the basics of chemical probing with click chemistry, and discuss its recent applications in the field of quantitative biology. Furthermore, I discuss the capability, significance, and future of the chemical probing of proteins, with an emphasis on the use of click chemistry in the field of the quantitative biology.

  14. Low-energy electron scattering from molecules, biomolecules and surfaces

    CERN Document Server

    Carsky, Petr


    Since the turn of the 21st century, the field of electron molecule collisions has undergone a renaissance. The importance of such collisions in applications from radiation chemistry to astrochemistry has flowered, and their role in industrial processes such as plasma technology and lighting are vital to the advancement of next generation devices. Furthermore, the development of the scanning tunneling microscope highlights the role of such collisions in the condensed phase, in surface processing, and in the development of nanotechnology.Low-Energy Electron Scattering from Molecules, Biomolecule

  15. Identification of Biomolecular Building Blocks by Recognition Tunneling: Stride towards Nanopore Sequencing of Biomolecules (United States)

    Sen, Suman

    DNA, RNA and Protein are three pivotal biomolecules in human and other organisms, playing decisive roles in functionality, appearance, diseases development and other physiological phenomena. Hence, sequencing of these biomolecules acquires the prime interest in the scientific community. Single molecular identification of their building blocks can be done by a technique called Recognition Tunneling (RT) based on Scanning Tunneling Microscope (STM). A single layer of specially designed recognition molecule is attached to the STM electrodes, which trap the targeted molecules (DNA nucleoside monophosphates, RNA nucleoside monophosphates or amino acids) inside the STM nanogap. Depending on their different binding interactions with the recognition molecules, the analyte molecules generate stochastic signal trains accommodating their "electronic fingerprints". Signal features are used to detect the molecules using a machine learning algorithm and different molecules can be identified with significantly high accuracy. This, in turn, paves the way for rapid, economical nanopore sequencing platform, overcoming the drawbacks of Next Generation Sequencing (NGS) techniques. To read DNA nucleotides with high accuracy in an STM tunnel junction a series of nitrogen-based heterocycles were designed and examined to check their capabilities to interact with naturally occurring DNA nucleotides by hydrogen bonding in the tunnel junction. These recognition molecules are Benzimidazole, Imidazole, Triazole and Pyrrole. Benzimidazole proved to be best among them showing DNA nucleotide classification accuracy close to 99%. Also, Imidazole reader can read an abasic monophosphate (AP), a product from depurination or depyrimidination that occurs 10,000 times per human cell per day. In another study, I have investigated a new universal reader, 1-(2-mercaptoethyl)pyrene (Pyrene reader) based on stacking interactions, which should be more specific to the canonical DNA nucleosides. In addition

  16. Consideration and Suggestion on Design of Mercury Removal Equipment%天然气脱汞装置设计的思考与建议

    Institute of Scientific and Technical Information of China (English)



    Chemisorption method is usually used for removing the mercury in natural gas. The characteristics, advantages & disadvantages and applicability of adsorbents used for mer-cury removal are compared and analyzed in this paper.The analysis shows that the metal sul-fide adsorbent has stronger adsorptive capacity and wider application scope. The locations for mercury removal equipment in the natural gas treatment process were compared and ana-lyzed . According to the result, removing mercury in the process front can reduce the harm of mercury at source, and has better application prospect. Meanwhile, twin tower in series for adsorption is recommended, in order to make full use of the adsorption capacity of mer-cury removal adsorbent. According to mercury distribution in the natural gas treatment pro-cess, the mercury limit value of natural gas should be determined by the principle that the mercury content in the exhaust gas and the commercial gas must be in accordance with the relevant regulations. Referenced with the theoretical calculation and the relevant standards, the limit value of the natural gas removal mercury equipment is designed to be 28 μg/m3 can meet the requirement of production.%天然气脱汞常采用化学吸附法,对比分析该方法中常用脱汞剂的特性、优缺点及适用范围,表明金属硫化物脱汞剂的脱汞能力更强、适用范围更广。针对天然气处理流程中脱汞装置的位置设置分析认为:采用工艺前端脱汞可以从源头降低汞所带来的危害,具有更好的应用前景;并推荐采用双塔串联吸附流程,以便充分利用脱汞剂的吸附容量;依据汞在天然气处理流程中的分布情况,提出天然气脱汞限值应以处理过程所产生的排放气和商品外输气汞含量符合相关规定为原则进行确定,参照相关规范及理论计算,天然气脱汞装置按脱汞限值28μg/m3进行设计可以满足生产要求。

  17. Raman spectroscopy detection of biomolecules in biocrusts from differing environmental conditions (United States)

    Miralles, I.; Jorge-Villar, S. E.; van Wesemael, B.; Lázaro, R.


    Lichens and cyanobacteria colonize inhospitable places covering a wide climate range due to their different survival strategies, such as the synthesis of protective biomolecules. The effect of ecological factors on the synthesis of biomolecules has not been widely analysed. This study aimed to assess the effects of four factors (species, microclimate, seasonality and hydration state) and their interactions on the biomolecule frequency detected by Raman Spectroscopy. We included cyanobacterial biocrusts, and the lichens Diploschistes diacapsis, Squamarina lentigera, and Lepraria isidiata; two contrasted microclimates (typical and marginal), two contrasted seasons (hot and dry vs cool and wet) and two hydration states (dry and wet). "Species" was the most influential factor in the identity and frequency of the main biomolecules. Microclimatic differences in the range of the local specific habitats only influenced the biomolecules in cyanobacteria. There was a quadruple interaction among the factors, the effects being different mainly depending on the species. At D. diacapsis, the production of their main biomolecules depended on microclimate, although it also depended on seasonality. Nevertheless, in L. isidiata and S. lentigera microclimatic differences did not significantly affect the production of biomolecules. In the lichen species, the microhabitats exposed to relatively larger incident radiation did not show significantly larger relative frequency of photoprotective biomolecules. No clear connection between higher production of oxalates and drier microhabitats was found, suggesting that the synthesis of oxalates is not related to water reserve strategy. The pros and cons of monitor biomolecules in biocrust by Raman spectrometry were also discussed.

  18. A transistor-based biosensor for the extraction of physical properties from biomolecules (United States)

    Kim, Sungho; Baek, David; Kim, Jee-Yeon; Choi, Sung-Jin; Seol, Myeong-Lok; Choi, Yang-Kyu


    An analytical technique is proposed that uses an asymmetric double-gate field-effect transistor (FET) structure to characterize the electrical properties of biomolecules, including their permittivity and charge density. Using a simple measurement with the proposed FET structure, we are able to extract the physical properties (i.e., permittivity and charge density) of biomolecules. A reliable analytical tool for the characterization of biomolecules can be provided by the proposed FET structure without a complex measurement system. It is expected that the proposed method will be expanded into a universal analysis technique for the electrical evaluation of biomolecules in applications beyond biosensing.

  19. Study of Integrated Equipment Support System Design Based on Data Warehouse%基于数据仓库的装备综合保障系统设计研究

    Institute of Scientific and Technical Information of China (English)

    代冬升; 谢峰; 孙江生; 梁伟杰


    目的:使各类装备保障信息系统中的装备保障数据得到综合利用,实现服务上级决策支持的功能。方法提出设计综合性的装备保障系统的构想,阐述构建装备保障数据仓库的总体方式,并针对体系结构、运行流程、中心数据库结构、运维管理等方面进行设计。结果形成了融入知识库等多数据库的扩展数据仓库,从理论的角度对构建装备数据仓库以支持。结论装备综合保障系统的设计思想与设计理论,为系统的最终实现奠定了理论基础,为推动装备保障信息化发展起到重要作用。%Objective To achieve comprehensive utilization of the equipment support data in various equipment support information systems, and realize the function of supporting leadership decisions. Methods The design of an integrated e-quipment support system was proposed. The overall approach of building equipment data warehouse was expounded and the architecture, running processes, structure of center database as well as running management were designed in this article. Results An expended data warehouse integrating multiple databases including knowledge database was formed, and the con-struction of equipment warehouse was supported from the theoretical point of view. Conclusion The design ideas and design theory of an integrated equipment support system laid a theoretical foundation for the final realization of the system, and is of great importance for promoting the development of equipment support information technology.

  20. Design Points for the Equipment and Piping Arrangement of Flammable Liquid Tank Farm%可燃液体罐区设备及管道布置设计要点

    Institute of Scientific and Technical Information of China (English)



    可燃液体罐区是化工装置不可或缺的重要设施,其设备选型、管道布置等在设计上是有较高要求的,正确的设备及管道布置对罐区起着至关重要的安全保障作用。本文以甲醇为例,介绍了可燃液体储罐的设计选型、容量确定、设备布置及与之相通管道的设计要点,可为今后的可燃液体储罐的相关设计提供参考与借鉴。%Flammable liquid tank farm is an indispensable facility in chemical devices. Equipment selection, piping arrangement etc. have to meet higher requirements. Correct equipment and piping arrangement play an important guarantee role in the safety of the farm. In this paper, the methanol plant was introduced as an example for the design of unit mode selection, capacity determination, equipment arrangement and piping arrangement. This will offer some references for the design of the equipment and piping arrangement in the flammable liquid tank farm.

  1. 一种机载设备的中央处理单元模块的设计与实现%Design and Implementation of a Central Process Unit in Airborne Equipment

    Institute of Scientific and Technical Information of China (English)

    王俊; 吕俊; 杨宁


    The design and implementation of a central process unit in airborne equipment is introduced in this paper. The airborne equipment receives instruction signals from flight control system via RS422 communication, then the central process unit implements controlling, data calculation, A/D conversion, and feedback the result to actuuating mechanism, so that implementing the expected functions of airborne equipment. The equipment has been used in the airborne which proves that this design is referential and practical.%文章介绍了一种机载设备的中央处理单元模块设计与实现。机载设备通过RS422通讯接收飞行控制系统发来的指令信号,中央处理单元完成控制、数据解算、A/D转换等功能,将结果反馈给执行机构,从而实现机载设备的预期功能。本设备已在飞机上使用,使用结果良好,因此具有较强的参考性和实用性。

  2. 选煤厂振动设备MEMS无线传感器振动节点设计%Design of Coal Preparation Plant Vibration Equipment MEMS Wireless Sensor Vibration Node

    Institute of Scientific and Technical Information of China (English)

    付涛; 黄根岭


    MEMS wireless sensor node is mainly used in coal preparation plant equipment to carry out the vibration condition of the comprehensive monitoring, in order to carry on comprehensive analysis, understand the running state of the equipment. This paper first briefly introduces the coal preparation plant vibration equipment MEMS wireless sensor vibration node design scheme, and then respectively from the hardware design and software design two aspects of the design scheme is discussed on the.%MEMS无线传感器节点主要用于对选煤厂设备的振动情况开展全面监测,以便进行综合分析,了解设备运行状态。本文首先简单介绍了选煤厂振动设备MEMS无线传感器振动节点设计方案,之后分别从硬件及软件两方面对设计方案进行了深入阐述。

  3. Biosurfactants: Multifunctional Biomolecules of the 21st Century

    Directory of Open Access Journals (Sweden)

    Danyelle Khadydja F. Santos


    Full Text Available In the era of global industrialisation, the exploration of natural resources has served as a source of experimentation for science and advanced technologies, giving rise to the manufacturing of products with high aggregate value in the world market, such as biosurfactants. Biosurfactants are amphiphilic microbial molecules with hydrophilic and hydrophobic moieties that partition at liquid/liquid, liquid/gas or liquid/solid interfaces. Such characteristics allow these biomolecules to play a key role in emulsification, foam formation, detergency and dispersal, which are desirable qualities in different industries. Biosurfactant production is considered one of the key technologies for development in the 21st century. Besides exerting a strong positive impact on the main global problems, biosurfactant production has considerable importance to the implantation of sustainable industrial processes, such as the use of renewable resources and “green” products. Biodegradability and low toxicity have led to the intensification of scientific studies on a wide range of industrial applications for biosurfactants in the field of bioremediation as well as the petroleum, food processing, health, chemical, agricultural and cosmetic industries. In this paper, we offer an extensive review regarding knowledge accumulated over the years and advances achieved in the incorporation of biomolecules in different industries.

  4. The nanoparticle biomolecule corona: lessons learned - challenge accepted? (United States)

    Docter, D; Westmeier, D; Markiewicz, M; Stolte, S; Knauer, S K; Stauber, R H


    Besides the wide use of engineered nanomaterials (NMs) in technical products, their applications are not only increasing in biotechnology and biomedicine, but also in the environmental field. While the physico-chemical properties and behaviour of NMs can be characterized accurately under idealized conditions, this is no longer the case in complex physiological or natural environments. Herein, proteins and other biomolecules rapidly bind to NMs, forming a protein/biomolecule corona that critically affects the NMs' (patho)biological and technical identities. As the corona impacts the in vitro and/or in vivo NM applications in humans and ecosystems, a mechanistic understanding of its relevance and of the biophysical forces regulating corona formation is mandatory. Based on recent insights, we here critically review and present an updated concept of corona formation and evolution. We comment on how corona signatures may be linked to effects at the nano-bio interface in physiological and environmental systems. In order to comprehensively analyse corona profiles and to mechanistically understand the coronas' biological/ecological impact, we present a tiered multidisciplinary approach. To stimulate progress in this field, we introduce the potential impact of the corona for NM-microbiome-(human)host interactions and the novel concept of 'nanologicals', i.e., the nanomaterial-specific targeting of molecular machines. We conclude by discussing the relevant challenges that still need to be resolved in this field.

  5. Novel in Vitro Efficiency of Chitosan Biomolecule Against Trichomonas Gallinae

    Directory of Open Access Journals (Sweden)

    SH Pourseyed


    Full Text Available Background: Development of new natural agents for parasitic diseases treatment has unexpectedly increased to overcome effectively against emergence and re-emergence of parasitic diseases, the appearance of drug resistant organisms and toxic side effects of current agents. The aim of the study was to evaluate antiprotozoal activities of chitosan biomolecule on trophozoites of Trichomonas gallinae.Methods: The antitrichomonal activity of various low molecular weight chitosan concentrations including 125, 250, 500 and 1250 μg ml-1 against T. gallinae trophozoites cultured in trypticase-yeast extract-maltose medium supplemented with heat-inactivated cold horse serum was evaluated in vitro. Samples containing medium without chitosan were also assayed as controls.Results: The mortality rates at 0, 3 and 6 h post treatment with all concentrations were significantly different from control group (P<0.05. Treated trophozoites showed more susceptibility to the highest concentration reaching mortality rate of 100% at 3h post inoculation. However, at this time, results for 125, 250 and 500 μg ml-1 were 93%, 95% and 96.7%, respectively.Conclusion: The results demonstrate that the application of chitosan biomolecule is a promising option for treatment of trichomoniasis in pigeons.

  6. Biosurfactants: Multifunctional Biomolecules of the 21st Century. (United States)

    Santos, Danyelle Khadydja F; Rufino, Raquel D; Luna, Juliana M; Santos, Valdemir A; Sarubbo, Leonie A


    In the era of global industrialisation, the exploration of natural resources has served as a source of experimentation for science and advanced technologies, giving rise to the manufacturing of products with high aggregate value in the world market, such as biosurfactants. Biosurfactants are amphiphilic microbial molecules with hydrophilic and hydrophobic moieties that partition at liquid/liquid, liquid/gas or liquid/solid interfaces. Such characteristics allow these biomolecules to play a key role in emulsification, foam formation, detergency and dispersal, which are desirable qualities in different industries. Biosurfactant production is considered one of the key technologies for development in the 21st century. Besides exerting a strong positive impact on the main global problems, biosurfactant production has considerable importance to the implantation of sustainable industrial processes, such as the use of renewable resources and "green" products. Biodegradability and low toxicity have led to the intensification of scientific studies on a wide range of industrial applications for biosurfactants in the field of bioremediation as well as the petroleum, food processing, health, chemical, agricultural and cosmetic industries. In this paper, we offer an extensive review regarding knowledge accumulated over the years and advances achieved in the incorporation of biomolecules in different industries.

  7. Design and Simulation of a Var Compensation Equipment%一种无功补偿装置设计与仿真

    Institute of Scientific and Technical Information of China (English)

    闫国琦; 庞树杰; 李继宇


    By establishing a non-inductive load filter capacitor current AC-DC switching circuit, the output lags the system voltage by 1/4 cycle of SPWM frequency control method and system to make inductive load energy exchange occurs, to replace the capacitor compensation system inductance inductive successful results. And through the Y/A three-phase transformer connected with the work of the single-phase compensation circuit, eliminating the larger single-phase compensation circuit of 3 harmonics, make compensation circuit excluding low-order harmonic. Based on this principle, a kind of reactive power compensation in power system of power capacitor using inductively instead of control method was proposed, and studies the reactive power compensation device parameters design. To reactive power compensator capacity and voltage, by reducing the impact of the current power capacitor, improving the working reliability and reduce the cost of equipment. Using the circuit simulation software, the compensation effect was tested, and the compensation method was demonstrated.%通过建立一个不含滤波电容的电感负载交直交换流电路,利用输出滞后系统电压1/4工频周期的SPWM控制方法,使电感负载与系统感性负载发生能量交换,达到电感代替电容补偿系统感性无功的效果.并通过Y/△三相变压器连接3个配合工作的单相补偿电路,消去单相补偿电路中较大的3次谐波,使三相补偿电路不含低次谐波.基于该原理,提出一种在电力系统无功补偿中利用电感代替功率电容的控制方法,并研究该无功补偿装置的参数设计.有利于无功补偿器容量和电压的提升,减少由功率电容带来的冲击电流,提高了工作可靠性和降低了设备成本.用仿真软件建立算例电路检验补偿效果,并论证了补偿方法.

  8. 电传动车辆显控终端设计%Display-Control Design of Terminal Equipment for Electric Drive Vehicles

    Institute of Scientific and Technical Information of China (English)

    初华; 刘春光; 袁东


    The display- control terminal equipment takes an important part in integrated control of electric drive vehicles. It is used as a window of human-computer interaction. Its functions includes that, the first is converting vehicle executable signal based on the driver's operation. The second is showing the vehicle' s running state parameters. The third is responsible for fault information diagnosis, display and storage. For these specific tasks, the first, this paper analyzes the basic structure of the electric drive vehicle. On this basis, we can see the functions plan of display-control terminal. Virtual meter displays the vehicle operating parameters. The second, this paper analyzes display -control terminal software design principles. Through buttons and touch screen operation reflects the intention of the driver. The last, this paper constitutes CAN-bus protocol and realizes the function for controlling and management. After testing, the display-control terminal runs well on electric drive vehicles.%显控终端是电传动车辆综合控制中的重要组成部分,其作为人机交互的窗口,不仅要将驾驶员的操作意图转换为整车可执行信号,而且需要把车辆运行的各项状态参数及时显示,还要负责故障信息的诊断、显示和存储;针对以上具体任务,首先分析电传动车辆的基本结构,在此基础上得出显控终端的功能规划,采用虚拟仪表显示车辆运行参数;其次分析了显控终端软件设计原则,通过按键和触摸屏反映驾驶员操作意图;最后制定CAN总线传输协议,实现控制管理功能;经过试验,本显控终端在电传动车辆上运行可靠.

  9. Monitoring Framework Design for Logistics Equipment Based on RFID%基于RFID的物流装备信息监控网络平台设计

    Institute of Scientific and Technical Information of China (English)

    刘富春; 周受钦


    Containers are the core equipment of the logistics transportation in the world,and over 90 percent of the international transportation is accomplished by containers. The effectiveness of logistics and custom clearance it due to the lag in technology of intelligence and informationization of the container transportation,and unable to monitor in visualized mode. It investigates the developments of the monitoring and management of the smart containers based on the RFID technique,and designs the system platform for internet of things based on the smart containers. This platform can make the overall supply chain information unopposed, and can enhance the effectiveness of the modern logistics. This research provides the perfect product and technique scheme for smart container applications.%集装箱是全球物流运输的核心装备,全球国际货运90%以上都是通过集装箱完成,目前集装箱运输的智能化和信息化技术落后,从而导致物流效率低、海关通关效率低且做不到实时可视化监控.文中基于射频识别和嵌入式徽控制器技术开发了用于托盘和集装箱等物流装备的智能数据采集终端,进而设计了基于智能集装箱的现代物流装备物联网系统平台.该平台的广泛应用使现代物流系统的整个供应链信息流畅通,实现了可视化监控,大幅度提高了现代物流效率.为行业应用提供智能集装箱成熟产品和技术方案.

  10. 大型在线设备管理中自动图表的设计%Design for Automatic Plot of Large On-line Equipment Management

    Institute of Scientific and Technical Information of China (English)



    大型在线设备通常工作在生产线或重要的流水线上,因此在实际的工作过程中对设备各部件的工作时间的要求非常严格,在设备的设计、生产、测试、监控和维护过程中都需要对设备的工作时间进行管理,传统的方法是通过软件将传感器数据或运行日志直接在Excel中记录时间数据,数据获得方式直接且方便,但是不利于人员理解数据含义。为了提高管理效率,提出了利用VBA技术开发根据数据自动绘制甘特图图表的方法。此法能够大幅提高数据的可读性,并大量减少人工工作量,为多个部门的管理、沟通工作带来极大方便。%Large on-line equipment usually works on a production line or an important pipeline , so the requirement on the working time of each component is very strict in practical working process .The time management is especially important during all processes of de-sign, production, test, monitoring and maintaining .The traditional method of time management is to directly record time data of sensors or logs into Excel sheets by software .This method of obtaining data is direct and convenient , but it goes against the comprehension of the information by the stuff .To improve management efficiency , this paper presents a method of drawing Gantt chart automatically ac-cording to data by using VBA technology , which increases the readability of massive data , reduces workload and brings convenience for management and communication in several departments of a company .

  11. 金属丝杨氏模量测量装置的设计%Design of the Measuring Equipment to Explore Wire Young’ s Modulus

    Institute of Scientific and Technical Information of China (English)

    李儒颂; 叶文江


    Based on Michelson interference principle,the measuring equipment to explore wire Young’ s modu-lus is designed. A continuous and uniform increase force applied to wire will induce a small elongation,which makes the moving mirror move by the sensitive lever and causes the change of optical screen interference ring number. The light intensity signal is detected by a photoelectric sensor,and then through the analog to digital conversion the optical signals are converted to electrical signals,in the end the small elongation of wire can be measured after calculating accurately the interference ring number. The external applied force can be directly measured by the force sensor. The software of Origin is used to process the experimental data to avoid the man-made error,and then the wire Young ¢s modulus can be calculated intelligently.%基于迈克尔逊干涉原理设计了金属丝杨氏模量的测量装置。在金属丝上施加持续而又均匀增加的力,引起金属丝细微伸长,通过灵敏杠杆带动移动镜的移动,从而引起光屏上干涉圆环数目的变化;用一种光电传感器捕捉干涉圆环的光强信号,将光信号转换为电信号,再通过模数转换,精确算出干涉圆环的条数,就可以实现对金属丝微小伸长量的测量。采用Origin软件处理实验数据,避免了人为因素所造成的误差,智能化计算出金属丝的杨氏模量。

  12. 医院卫生装备质量管理系统的设计与实现%Design and Realization of Medical Equipment Quality Management System for Hospital

    Institute of Scientific and Technical Information of China (English)

    蒋元林; 潘晓东; 周耀崇; 喻志才; 蒋俊成


    Objective To design a medical equipment quality management system. Methods With Power Builder as the programming tool, the equipment account was used as the core part to establish medical equipment databases, and thus the records and reports came into birth, with the following involved such as the equipment application, type selection demonstration, original record, quality control inspection report and statistical analysis. Results Dynamic management of all links of medical equipment was realized, with the work load of the staff decreased and the hospital quality management system built. Conchustion The whole-process and all-elements management of medical equipment is implemented with the system.%目的:设计一套医疗卫生装备质量管理系统.方法:采用Power Builder编程,以设备台账为操作基础及核心,建立卫生装备相关数据库,由此衍生出所有的记录和报告内容,包括设备的申请、选型论证、原始记录及计量质控检测报告以及相关统计分析功能.结果:该系统实现了对医疗卫生装备各个环节的动态跟踪管理,减轻了管理人员的工作量,并进一步完善了医院的质量管理体系.结论:该系统规范了医疗卫生装备“全过程、全要素”的质量管理工作,确保医疗卫生装备使用的安全有效,有较强的实用性、可行性.

  13. A photocleavable affinity tag for the enrichment of alkyne-modified biomolecules

    NARCIS (Netherlands)

    Koopmans, Timo; Dekker, Frank J.; Martin, Nathaniel I.


    A new photocleavable affinity tag for use in the enrichment of alkyne-labelled biomolecules is reported. The tag is prepared via a concise synthetic route using readily available materials. The photolytic conditions employed for cleavage of the tag provide for a clean release of enriched biomolecule

  14. High-Speed AFM Reveals the Dynamics of Single Biomolecules at the Nanometer Scale

    NARCIS (Netherlands)

    Katan, A.J.; Dekker, C.


    Atomic force microscopy allows visualization of biomolecules with nanometer resolution under physiological conditions. Recent advances have improved the time resolution of the technique from minutes to tens of milliseconds, meaning that it is now possible to watch single biomolecules in action in re

  15. Sustained delivery of biomolecules from gelatin carriers for applications in bone regeneration

    NARCIS (Netherlands)

    Song, J.; Leeuwenburgh, S.C.G.


    Local delivery of therapeutic biomolecules to stimulate bone regeneration has matured considerably during the past decades, but control over the release of these biomolecules still remains a major challenge. To this end, suitable carriers that allow for tunable spatial and temporal delivery of biomo

  16. Abyssal soil investigation equipment

    Energy Technology Data Exchange (ETDEWEB)

    Smits, F.P.; Maggioni, W.; Mainardi, U. [ISMES, Bergamo (Italy)


    The present paper deals with the development of a testing device for quick offshore geotechnical investigations. The equipment, at present, consists of a penetrometer, mounted on a sea bed platform, a handling frame and a control cabin; a further development includes a drill rig and a geotechnical laboratory. All this is designed so as to be an autonomous unit that can be handled by various types of non-specialized ships which have the necessary deck space. The sea bed platform can operate in 2,000 m of waterdepth and is provided with a hydraulic penetrometer, with a thrust capacity of 400 kN, able to carry out cone penetration type testing until a depth of more than 100 m, as well as shallow depth continuous sampling. The penetrometer can be equipped with a series of probes (piezocone, pressiocone, total radial pressure transducer, seismic cone) allowing the execution of the wide range of direct geotechnical measurements normally performed during on-land penetration tests.

  17. 加注设备虚拟装配仿真训练系统设计%Design of Virtual Assembly Simulation and Training System of Propellant Fueling Equipments

    Institute of Scientific and Technical Information of China (English)

    陈家照; 廖斯宏; 张玉祥


    The paper designs the function and technical frame of the virtual assembly simulation and training system of rocket propellant fueling equipment, builds the 3D solid models of the fueling equipment,develops the assembly and disassembly demonstration module, interactive assembly operating module and assembly technology planning module of the fueling equipment, and edits the module management program. The system is of great help to the vocational study and skill training of the propellant fueling equipment operators and repair personnel.%设计了火箭推进剂加注设备虚拟装配仿真训练系统的功能和技术框架,建立了加注设备的三维实体模型,开发了加注设备的拆装演示、交互装配操作和装配工艺规划模块,并编制了功能模块调度与管理界面程序.设计的系统对加注设备操作和维修人员的业务学习和技能训练有重要帮助.

  18. Superficially porous particles with 1000Å pores for large biomolecule high performance liquid chromatography and polymer size exclusion chromatography. (United States)

    Wagner, Brian M; Schuster, Stephanie A; Boyes, Barry E; Shields, Taylor J; Miles, William L; Haynes, Mark J; Moran, Robert E; Kirkland, Joseph J; Schure, Mark R


    To facilitate mass transport and column efficiency, solutes must have free access to particle pores to facilitate interactions with the stationary phase. To ensure this feature, particles should be used for HPLC separations which have pores sufficiently large to accommodate the solute without restricted diffusion. This paper describes the design and properties of superficially porous (also called Fused-Core(®), core shell or porous shell) particles with very large (1000Å) pores specifically developed for separating very large biomolecules and polymers. Separations of DNA fragments, monoclonal antibodies, large proteins and large polystyrene standards are used to illustrate the utility of these particles for efficient, high-resolution applications.

  19. Conveniently assembling dithiocarbamate and gold nanoparticles onto the gold electrode: A new type of electrochemical sensors for biomolecule detection (United States)

    Li, Maoguo; Gao, Feng; Yang, Ping; Wang, Lun; Fang, Bin

    Dithiocarbamate and gold nanoparticles have been successfully assembled onto the surface of the gold electrode and a novel ultrastable chemical modified electrode (CME) was fabricated conveniently. The as-prepared CME was investigated by electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM) and quartz crystal microbalance (QCM), and its electrochemical behaviors for catalytic oxidation of dopamine (DA) was also observed by cyclic voltammetry (CV) and amperometric i- t curve. The results indicated that the novel surface has endowed the electrode with not only ultrastability but also the advantages of organic ligands and gold nanoparticles, which open up a new way to design high efficient and utility electrochemical sensors for biomolecule detection.

  20. Sustained delivery of biomolecules from gelatin carriers for applications in bone regeneration. (United States)

    Song, Jiankang; Leeuwenburgh, Sander Cg


    Local delivery of therapeutic biomolecules to stimulate bone regeneration has matured considerably during the past decades, but control over the release of these biomolecules still remains a major challenge. To this end, suitable carriers that allow for tunable spatial and temporal delivery of biomolecules need to be developed. Gelatin is one of the most widely used natural polymers for the controlled and sustained delivery of biomolecules because of its biodegradability, biocompatibility, biosafety and cost-effectiveness. The current study reviews the applications of gelatin as carriers in form of bulk hydrogels, microspheres, nanospheres, colloidal gels and composites for the programmed delivery of commonly used biomolecules for applications in bone regeneration with a specific focus on the relationship between carrier properties and delivery characteristics.

  1. A new algorithm for construction of coarse-grained sites of large biomolecules. (United States)

    Li, Min; Zhang, John Z H; Xia, Fei


    The development of coarse-grained (CG) models for large biomolecules remains a challenge in multiscale simulations, including a rigorous definition of CG representations for them. In this work, we proposed a new stepwise optimization imposed with the boundary-constraint (SOBC) algorithm to construct the CG sites of large biomolecules, based on the s cheme of essential dynamics CG. By means of SOBC, we can rigorously derive the CG representations of biomolecules with less computational cost. The SOBC is particularly efficient for the CG definition of large systems with thousands of residues. The resulted CG sites can be parameterized as a CG model using the normal mode analysis based fluctuation matching method. Through normal mode analysis, the obtained modes of CG model can accurately reflect the functionally related slow motions of biomolecules. The SOBC algorithm can be used for the construction of CG sites of large biomolecules such as F-actin and for the study of mechanical properties of biomaterials.

  2. Efficient extraction strategies of tea (Camellia sinensis) biomolecules. (United States)

    Banerjee, Satarupa; Chatterjee, Jyotirmoy


    Tea is a popular daily beverage worldwide. Modulation and modifications of its basic components like catechins, alkaloids, proteins and carbohydrate during fermentation or extraction process changes organoleptic, gustatory and medicinal properties of tea. Through these processes increase or decrease in yield of desired components are evident. Considering the varied impacts of parameters in tea production, storage and processes that affect the yield, extraction of tea biomolecules at optimized condition is thought to be challenging. Implementation of technological advancements in green chemistry approaches can minimize the deviation retaining maximum qualitative properties in environment friendly way. Existed extraction processes with optimization parameters of tea have been discussed in this paper including its prospects and limitations. This exhaustive review of various extraction parameters, decaffeination process of tea and large scale cost effective isolation of tea components with aid of modern technology can assist people to choose extraction condition of tea according to necessity.

  3. Synthesis of selenium nanorods with assistance of biomolecule

    Indian Academy of Sciences (India)

    S Kannan; K Mohanraj; K Prabhu; S Barathan; G Sivakumar


    Nanorods of one-dimensional (1D) trigonal selenium (t-Se) are synthesized using biomolecule substances for five different aging times (1 h, 2 h, 3 h, 1 day and 4 days) by precipitation method. XRD analysis indicates a shift of the (1 0 1) plane towards higher diffraction angle for 1 day aging time. It is observed that the crystallite size decreases with increase in aging time except for an aging period of 4 days. FTIR analysis confirmed that the presence of stretching and bending vibrations of Se–O in both synthesized and commercial selenium samples at 465, 668 and 1118 cm-1. The FESEM micrographs are evident for the changes of rod size as a function of aging time. It is observed that the optical band gap energy is increased with aging time up to 1 day, whereas it decreases in 4 days aging time.

  4. Click-EM for imaging metabolically tagged nonprotein biomolecules. (United States)

    Ngo, John T; Adams, Stephen R; Deerinck, Thomas J; Boassa, Daniela; Rodriguez-Rivera, Frances; Palida, Sakina F; Bertozzi, Carolyn R; Ellisman, Mark H; Tsien, Roger Y


    EM has long been the main technique for imaging cell structures with nanometer resolution but has lagged behind light microscopy in the crucial ability to make specific molecules stand out. Here we introduce click-EM, a labeling technique for correlative light microscopy and EM imaging of nonprotein biomolecules. In this approach, metabolic labeling substrates containing bioorthogonal functional groups are provided to cells for incorporation into biopolymers by endogenous biosynthetic machinery. The unique chemical functionality of these analogs is exploited for selective attachment of singlet oxygen-generating fluorescent dyes via bioorthogonal 'click chemistry' ligations. Illumination of dye-labeled structures generates singlet oxygen to locally catalyze the polymerization of diaminobenzidine into an osmiophilic reaction product that is readily imaged by EM. We describe the application of click-EM in imaging metabolically tagged DNA, RNA and lipids in cultured cells and neurons and highlight its use in tracking peptidoglycan synthesis in the Gram-positive bacterium Listeria monocytogenes.

  5. Biomolecule Profiles in Inedible Wild Mushrooms with Antioxidant Value

    Directory of Open Access Journals (Sweden)

    Isabel C.F.R. Ferreira


    Full Text Available The use of natural products isolated from mushrooms, included inedible species, against infection, cancer diseases and other oxidative-stress related diseases is one of the cornerstones of modern medicine. In the present work, the antioxidant molecule profiles of inedible mushroom species were evaluated and compared with those of edible species. The order of antioxidant abundance found in inedible wild mushrooms was: phenolics > flavonoids > ascorbic acid > tocopherols > carotenoids, similar to that of edible species. Furthermore the same energetic biomolecules were found including the disaccharide trehalose, the monosaccharide alcohol derivative mannitol and the fatty acids palmitic, oleic and linoleic acids. Fomitopsis pinicola revealed a very high phenolics concentration (388 mg GAE/g extract and powerful antioxidant properties, mainly reducing power (EC50 value 60 μg/mL similar to the standard Trolox®. It could find applications in the prevention of free radical-related diseases as a source of bioactive compounds.

  6. Study, design and integration of an FPGA-based system for the time-of-flight calculation applied to PET equipment (United States)

    Aguilar Talens, D. Albert

    Nuclear Medicine has undergone significant advances in recent years due to improvements in materials, electronics, software techniques, processing etc., which has allowed to considerably extend its application. One technique that has progressed in this area has been the Positron Emission Tomography (PET) based on a non-invasive method with its especial relevance in the evaluation of cancer diagnosis and assessment, among others. This system is based on the principle of data collection and processing from which images of the spatial and temporal distribution of the metabolic processes that are generated inside the body are obtained. The imaging system consists of a set of detectors, normally placed in a ring geometry, so that each one provides information about events that have occurred inside. One of the reasons that have significantly evolved in PET systems is the development of techniques to determine the Time-of-Flight (TOF) of the photons that are generated due to the annihilation of positrons with their antiparticle, the electron. Determining TOF allows one for a more precise location of the events that are generated inside the ring and, therefore, facilitates the task of image reconstruction that ultimately use the medical equipment for the diagnosis and/or treatment. This Thesis begins with the assumption of developing a system based on Field Programmable Gate Arrays (FPGAs) for the integration of a Time- to-Digital Converter (TDC) in order to precisely carry out time measurements. This would permit the estimation of the TOF of the gamma particles for subsequent application in PET systems. First of all, the environment for the application is introduced, justifying the need of the purposed system. Following, the basic principles of PET and the state-of-the-art of similar systems are introduced. Then, the principles of Time-of-Flight based on FPGAs are discussed, and the adopted scheme explained, going into detail in each of its parts. After the development

  7. Recent Advances in Protein Extraction and Chiral Separation of Biomolecules

    Institute of Scientific and Technical Information of China (English)


    Reverse micelles create unique environment in organic media. They are capable of solubilizing hydrophilic biomolecules (e.g., proteins, peptides, amino acids, and DNAs) in their aqueous interior. This feature brings about the practical use of biomaterials in organic media because reverse micelles solubilize them with the intrinsic activity. In this paper, we focus on recent two topics concerning protein extraction and chiral separation of biomolecules using liquid membranes. In the first topic, we present recent attempts to extract proteins from an aqueous solution into isooctane using reverse micelles, and some important operational parameters to achieve an efficient protein transfer are discussed. Furthermore, novel function of reverse micelles as a protein activation medium is introduced. In the reverse micellar phase, denatured proreins were completely reactivated in the reverse micellar solution. The reverse micellar technique is found to be a useful tool not only for protein separation but also for protein refolding. Furthermore, we found that a cyclic ligand carixarene has an extraction ability to set up optimum conditions for protein transfer. In the second topic, we have found that a supported liquid membrane (SLM) encapsulating enzymes shows high enantioselectivity (enantioselective excess value is over 96%) in the transport of racemic pharmaceutical compound ibuprofen. A different experiment also suggests that the α-chymotrypsin-catalyzed reactions droved the enantioselective transport of L-phenylalanine based on the enantioselectivity of the enzyme. The SLM encapsulating the surfactant-enzyme complex enabled the highly enantioselective separation of racemic mixtures. It can be envisioned that arrangement of appropriate enzymes in the SLM system will allow enantioselective separation of various useful organic compounds.

  8. 基于GB9706.1的医用电气设备的设计要点分析%Analysis of the Key Points in Design of Medical Electrical Equipment Based on GB9706.1

    Institute of Scientific and Technical Information of China (English)



    In combination with the standard GB 9706.1-2007 Medical Electrical Equipment Part 1:General Safety Requirements, this paper summarized and analyzed the principle and key points in design of medical electrical equipment from the perspective of safety, including design of power supply, application, over-current protection devices, equipment structure and layouts, interfaces as well as the requirements of hazard prevention and bio-safety.%本文主要从安全角度出发,结合标准GB9706.1-2007《医用电气设备第1部分:安全通用要求》,总结并分析了医用电气设备应遵循的设计原则及产品设计阶段需要重点考虑的内容,包括电源部分设计、应用部分设计、过电流保护装置设计、设备结构和布局设计、接口设计、对危险的防护及生物安全性要求。

  9. Anti-cross-infection Design and Standard Requirements of Dental Devices and Equipments%口腔器械设备防交叉感染设计与标准要求

    Institute of Scientific and Technical Information of China (English)



    This paper analyzed the main factors that cause cross-infection of oral clinical, and presented the design requirements of anti-cross-infection dental devices and equipments. It focused on the requirements of dental devices and equipments standards involved in anti-cross-infection, as wel as the latest international standardization progress.%  本文分析了引起口腔临床交叉感染的主要因素,提出了口腔器械设备防交叉感染的设计要求,重点讲述了口腔器械设备涉及防交叉感染的标准要求,以及国际最新标准化进展。

  10. 某型火炮火控系统综合控制箱测试设备的设计研究磁%Design of Test Equipment of the Comprehensive Control Box of A Gun's Fire Control System

    Institute of Scientific and Technical Information of China (English)



    运用虚拟仪器技术和PXI总线技术对某型火炮火控系统综合控制箱检测设备的设计进行了研究。设计的检测设备能够完成对综合控制箱进行部件级的性能检测、故障诊断与模拟、维修支持等功能。%A test equipment is designed for comprehensive control box of certain gun's fire control system based on PXI bus and virtual instrument technology .This test equipment has the function of performance test ,fault diagnosis and simula‐tion ,maintenance support for comprehensive control box on component‐level .

  11. Design and Experiment on Coating Granulation Equipment for Tomato Seeds%番茄种子包衣丸粒化装置的设计与试验

    Institute of Scientific and Technical Information of China (English)

    孙正; 李树君; 苑严伟; 张俊宁; 董鑫


    为解决番茄种子扁圆轻薄、人工播种效率低及无法机械化播种等问题,设计了一种番茄种子自动包衣丸粒化装置,并利用该装置进行了试验。该装置主要由喂料器、转盘、滚筒、供液设备、控制箱及支座组成。在5g番茄种子与400 r/min的供液泵转速条件下,以滚筒转速、包衣时间、包衣剂质量及胶悬液体积为试验因素进行了正交与回归试验,并通过DPS数据处理软件对试验数据进行统计分析,得出试验范围内试验因素对丸粒化后种子抗压强度、有仔率和单仔率等指标的影响规律。同时,优化确定了包衣装置包衣丸粒化番茄种子的最佳参数组合:在滚筒转速为500r/min、包衣时间为300s、包衣剂质量为55g及胶悬液体积为40mL时,抗压强度、有仔率、单仔率分别为316.8 g、87%、93%。该研究可为番茄、辣椒等茄果科种子包衣丸粒化的研究提供参考。%Coating granulation plays an important role in the process of seeds treatment , coating seeds can reduce the la-bor intensity , improve the production efficiency and ensure the germination rate .Tomato planting area is very large in China , and is one of three major planting area over the world .In 2014 , the planting area was one million square hectare in China .However , at present , there is no proven technology available in tomato seeds coating , which brought about huge waste of seeds and caused a lot of economic losses .Therefore , it is very important and urgent to study the coating granulation technology for the tomato seeds .Based on the situation above , and in order to realize the mechanical sowing of the tomato seeds, the coating equipment was designed, suited for tomato seeds.The mechanism consisted of the feeder, roller, liquid supply device , a control box and support devices .The feeder transported regularly the tomato seeds and the coating agents into the roller , at the same time

  12. 基于Access数据库手术室医疗设备管理系统的设计与实现%Design and Implementation of an Access-Database-Based Operating Room Medical Equipment Management System

    Institute of Scientific and Technical Information of China (English)



    Objective To design a management system to provide informationalized management for operating room medical equipment.Methods Based on the actual situation of operating room medical equipment management and the access database platform, a database was designed for management of medical equipment maintenance, accessory procurement and entry into & exit from the operating room.Results This system had realized the effective management of operating room medical equipment, and improved its informatization level. Conclusion This system demonstrated its superiority in friendly interface, stable functions, easy-to-use features.%目的:设计一种简易的手术室医疗设备管理系统,为手术室医疗设备提供信息化管理手段。方法依据我院手术室医疗设备管理的实际情况,基于Access数据库平台,为手术室医疗设备的维修管理、配件的采购管理、设备进出手术室管理等相间数据信息建立数据库。结果该系统实现了对手术室医疗设备高效管理,有效地提高了手术室管理的信息化水平。结论该系统界面友好,功能稳定,使用简单方便。

  13. 四轮转向挂装车液压系统的设计%The Design of Hydraulic System of a Four-wheel-steering Loading Truck for Equipment Hanging on Aircraft

    Institute of Scientific and Technical Information of China (English)

    洪曼; 王志; 陈文科; 温琦; 杨力


    该文主要介绍了四轮驱动挂装车液压系统的设计原理,简要介绍了挂装车的结构设计。该挂装车采用后轮驱动、四轮转向的方式,其工作装置可以在6个自由度上进行调整,并在驻车制动、工作装置液压系统中设置应急系统,确保挂装车的作业安全。样机的研制成功证明了该挂装车液压系统的设计是合理、适用的。%This paper describes the design of the hydraulic system of the loading truck for equipment hanging on aircraft with four wheels steering. And the paper describes the design of the truck’s structure. The truck which with rear-wheel-drive and four-wheel-steering has a hanging structure which with 6-freedom. For the safety of the truck during hanging equipment on aircraft, there is an emergency hydraulic-circuit connecting to the parking hydraulic-circuit and the hanging hydraulic-circuit. The success of designing and producing the first truck proves that the design of hydraulic system of the truck is logical and useful.

  14. [Novel Hyphenated Techniques of Atomic Spectrometry for Metal Species Interaction with Biomolecules]. (United States)

    Li, Yan; Yan, Xiu-ping


    Trace metals may be adopted by biological systems to assist in the syntheses and metabolic functions of genes (DNA and RNA) and proteins in the environment. These metals may be beneficial or may pose a risk to humans and other life forms. Novel hybrid techniques are required for studies on the interaction between different metal species and biomolecules, which is significant for biology, biochemistry, nutrition, agriculture, medicine, pharmacy, and environmental science. In recent years, our group dwells on new hyphenated techniques based on capillary electrophoresis (CE), electrothermal atomic absorption spectrometry (ETAAS), and inductively coupled plasma mass spectroscopy (ICP-MS), and their application for different metal species interaction with biomolecules such as DNA, HSA, and GSH. The CE-ETAAS assay and CE-ICP-MS assay allow sensitively probing the level of biomolecules such as DNA damage by different metal species and extracting the kinetic and thermodynamic information on the interactions of different metal species with biomolecules, provides direct evidences for the formation of different metal species--biomolecule adducts. In addition, the consequent structural information were extracted from circular dichroism (CD) and X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, and Fourier transform infrared (FTIR) spectroscopy. The present works represent the most complete and extensive study to date on the interactions between different metal species with biomolecules, and also provide new evidences for and insights into the interactions of different metal species with biomolecules for further understanding of the toxicological effects of metal species.

  15. Comprehensive spectroscopic studies on the interaction of biomolecules with surfactant detached multi-walled carbon nanotubes. (United States)

    Sekar, Gajalakshmi; Mukherjee, Amitava; Chandrasekaran, Natarajan


    This paper investigates the interaction of ten diverse biomolecules with surfactant detached Multi-Walled Carbon Nanotubes (MWCNTs) using multiple spectroscopic methods. Declining fluorescence intensity of biomolecules in combination with the hyperchromic effect in UV-Visible spectra confirmed the existence of the ground state complex formation. Quenching mechanism remains static and non-fluorescent. 3D spectral data of biomolecules suggested the possibilities of disturbances to the aromatic microenvironment of tryptophan and tyrosine residues arising out of CNTs interaction. Amide band Shifts corresponding to the secondary structure of biomolecules were observed in the of FTIR and FT-Raman spectra. In addition, there exists an increased Raman intensity of tryptophan residues of biomolecules upon interaction with CNTs. Hence, the binding of the aromatic structures of CNTs with the aromatic amino acid residues, in a particular, tryptophan was evidenced. Far UV Circular spectra have showed the loss of alpha-helical contents in biomolecules upon interaction with CNTs. Near UV CD spectra confirmed the alterations in the tryptophan positions of the peptide backbone. Hence, our results have demonstrated that the interaction of biomolecules with OH-MWCNTs would involve binding cum structural changes and alteration to their aromatic micro-environment.

  16. Electrochemical immobilization of biomolecules on gold surface modified with monolayered L-cysteine

    Energy Technology Data Exchange (ETDEWEB)

    Honda, Mitsunori, E-mail:; Baba, Yuji; Sekiguchi, Tetsuhiro; Shimoyama, Iwao; Hirao, Norie


    Immobilization of organic molecules on the top of a metal surface is not easy because of lattice mismatch between organic and metal crystals. Gold atoms bind to thiol groups through strong chemical bonds, and a self-assembled monolayer of sulfur-terminated organic molecules is formed on the gold surface. Herein, we suggested that a monolayer of L-cysteine deposited on a gold surface can act as a buffer layer to immobilize biomolecules on the metal surface. We selected lactic acid as the immobilized biomolecule because it is one of the simplest carboxyl-containing biomolecules. The immobilization of lactic acid on the metal surface was carried out by an electrochemical method in an aqueous environment under the potential range varying from − 0.6 to + 0.8 V. The surface chemical states before and after the electrochemical reaction were characterized using X-ray photoelectron spectroscopy (XPS). The N 1s and C 1s XPS spectra showed that the L-cysteine-modified gold surface can immobilize lactic acid via peptide bonds. This technique might enable the immobilization of large organic molecules and biomolecules. - Highlights: • Monolayer l-cysteine deposited on Au surface as a buffer layer to immobilize biomolecules. • Lactic acid as the immobilized biomolecule as it is simple carboxyl-containing biomolecule. • X-ray photoelectron spectroscopy (XPS) of surface chemical states, before and after. • L-cysteine-modified Au surface can immobilize lactic acid via peptide bonds.

  17. Renewal of radiological equipment. (United States)


    In this century, medical imaging is at the heart of medical practice. Besides providing fast and accurate diagnosis, advances in radiology equipment offer new and previously non-existing options for treatment guidance with quite low morbidity, resulting in the improvement of health outcomes and quality of life for the patients. Although rapid technological development created new medical imaging modalities and methods, the same progress speed resulted in accelerated technical and functional obsolescence of the same medical imaging equipment, consequently creating a need for renewal. Older equipment has a high risk of failures and breakdowns, which might cause delays in diagnosis and treatment of the patient, and safety problems both for the patient and the medical staff. The European Society of Radiology is promoting the use of up-to-date equipment, especially in the context of the EuroSafe Imaging Campaign, as the use of up-to-date equipment will improve quality and safety in medical imaging. Every healthcare institution or authority should have a plan for medical imaging equipment upgrade or renewal. This plan should look forward a minimum of 5 years, with annual updates. Teaching points • Radiological equipment has a definite life cycle span, resulting in unavoidable breakdown and decrease or loss of image quality which renders equipment useless after a certain time period.• Equipment older than 10 years is no longer state-of-the art equipment and replacement is essential. Operating costs of older equipment will be high when compared with new equipment, and sometimes maintenance will be impossible if no spare parts are available.• Older equipment has a high risk of failure and breakdown, causing delays in diagnosis and treatment of the patient and safety problems both for the patient and the medical staff.• Every healthcare institution or authority should have a plan for medical imaging equipment upgrade or replacement. This plan should look forward a

  18. Formation of simple biomolecules from alanine in ocean by impacts (United States)

    Umeda, Y.; Sekine, T.; Furukawa, Y.; Kakegawa, T.; Kobayashi, T.


    The biomolecules on the Earth are thought either to have originated from the extraterrestrial parts carried with flying meteorites or to have been formed from the inorganic materials on the Earth through given energy. From the standpoint to address the importance of impact energy, it is required to simulate experimentally the chemical reactions during impacts, because violent impacts may have occurred 3.8-4.0 Gyr ago to create biomolecules initially. It has been demonstrated that shock reactions among ocean (H2O), atmospheric nitrogen, and meteoritic constitution (Fe) can induce locally reduction environment to form simple bioorganic molecules such as ammonia and amino acid (Nakazawa et al., 2005; Furukawa et al., 2009). We need to know possible processes for alanine how chemical reactions proceed during repeated impacts and how complicated biomolecules are formed. Alanine can be formed from glycine (Umeda et al., in preparation). In this study, we carried out shock recovery experiments at pressures of 4.4-5.7 GPa to investigate the chemical reactions of alanine. Experiments were carried out with a propellant gun. Stainless steel containers (30 mm in diameter, 30 mm long) with 13C-labeled alanine aqueous solution immersed in olivine or hematite powders were used as targets. Air gap was present in the sample room (18 mm in diameter, 2 mm thick) behind the sample. The powder, solution, and air represent meteorite, ocean, and atmosphere on early Earth, respectively. Two powders of olivine and hematite help to keep the oxygen fugacity low and high during experiments, respectively in order to investigate the effect of oxygen fugacity on chemical processes of alanine. The recovered containers, after cleaned completely, were immersed into liquid nitrogen to freeze sample solution and then we drilled on the impact surface to extract water-soluble run products using pure water. Thus obtained products were analyzed by LC/MS for four amino acids (glycine, alanine, valine, and

  19. 面向寿命周期的装备资源性设计策略研究%A Study on Life Cycle-Oriented Equipment Resource-Ability Design Strategy

    Institute of Scientific and Technical Information of China (English)

    刘世伦; 倪明仿; 王生凤; 辛涛


    Resource-ability is an attribute which is closely related to resources and the environment. Resource-ability design is a fundamental way to implement equipment resource-ability. Resource-ability design should fully account for the equipment total life cycle that from resource acquisition to retired or scrap phrase, and implement different layout strategies in terms of the characteristics for different stages. Considering the constraints of traditional fossil energy, metal resources, environmental bearing capacity, and other respects that we are faced with,resource-oriented design tends to select the material and energy type. It enables one to select the type that is of the ability to achieve sustainability and select green clean material or energy type,which can reduce the dependence of equipment on scarce resources, improve the environment-friendly attribute of equipment, and improve the energy consumption structure as well. Manufacture oriented design primarily places particular emphasis on the design for manufacturability in order to produce the equipment with the lowest expenses, consumption and the shortest time, and achieve necessary quality and performance demand. Packaging and transporting-oriented design primarily places particular emphasis on the design for green packageability. Maintenance-support oriented design puts particular emphasis on the design for the level of energy consumption and green maintainability, including the control and optimization of the energy consumption level of equipment, structure simplification, maintaining reachability,testability, prevention against error, resource recycling ability, and environmental and personnel security. Upgrade and transform oriented design puts particular emphasis on the design for extensibility. It is mainly concerned with changing and installing additional and transforming functions of the equipment system in the future during the course of design, so as to take relevant design measures to guarantee

  20. Design and Application of Auto-continuous Steering Dough Press Equipment%自动折叠连续轧面机的设计与应用

    Institute of Scientific and Technical Information of China (English)

    杨业栋; 闫金成; 胡冬泉; 柴松敏


    Research and develop an equipment which can continuous press dough.A series of circulate pressing process including delivering the dough into feeder using press roller,swerving of conveyor belts,fold-ing the dough using bi-directional interval folding belts and transferring of the dough back to feeder.The dough can form thousands to millions of layers of gluten structure through those processes.Practicals show that if dough processed using this equipment were used for steamed bread product,the quality is beyond the conven-tional products,even the hand-made product.%研制开发了一种可以对面团进行连续压延,使之形成均匀完善面筋网络的设备,并用于馒头生产提高产品品质。利用入口压辊滚压进面、转向输送带转向、正反运转输送带折叠、翻转压辊滚压转向、再通过输送带送回到入口压辊实现连续循环压延。应用表明本设备所加工的馒头品质优于普通机制及手工制作馒头。

  1. 电信设备软件自动升级设计与实现%Design and Implementation of the Software Automatic Upgrading for Telecom Equipments

    Institute of Scientific and Technical Information of China (English)



    针对电信设备在日常维护中需要人工对各种业务板进行频繁的软件版本升级的情况,作者提出了自动升级的设想。通过在系统中增加一个自动升级服务器,同时在设备主控板中增加相应的版本检测算法,实现对各类业务板的软件进行检测并自动升级的功能,文中一并给出了它的实现方式。%Aiming at the situation that the software versions of various service boards need to be upgraded frequently by manual in the routine maintenance of telecom equipments, the paper proposes a method to upgrade the software version automatically. An automatic upgrading server is introduced in telecom system; also a software version detection algorithm is added in the system control board, so as to implement the software version detection and automatic upgrading functions for all kinds of service boards in telecom equipment.

  2. Laser-guided direct writing: a novel method to deposit biomolecules for biosensors arrays. (United States)

    Xu, Juntao; Grant, Sheila A; Pastel, Robert L


    In this paper, we present a potential biomolecular patterning method, laser-guided direct writing guidance (LGDW), which may be utilized to deposit organic and bioactive particles for biosensor arrays. The instrumentation and operation of the LGDW system is introduced and the system settings used to achieve deposition are reported. The biomolecule, avidin, was deposited onto a substrate using LGDW to evaluate the possible damage from the laser on the biomolecules. The functionality of avidin after laser-based guidance was examined by exposing the deposited avidin molecules to its ligand, biotin. The results show some avidin retained its affinity to biotin after LGDW demonstrating little damage to the biomolecules.

  3. Biomolecule-directed assembly of binary gold and titanium dioxide nanoparticles. (United States)

    Zhu, Xiaoli; Chen, Zhenyu; Zhang, Xin; Zhu, Zhiqiang; Li, Genxi


    We report in this paper a novel strategy for "bottom-up" assembly of two types of metallic and semiconductor nanoparticles. By only using a small biomolecule, coenzyme A, as a "linker" to direct the assembly of metallic gold and semiconductor titanium dioxide nanoparticles, the usual biomolecule-directed system can be greatly simplified. Effects of experimental conditions on the formation of binary nanoparticles are studied. This strategy using single small biomolecule to direct assembly of nanoparticles is proven to be efficient, facile and non-toxic and should be extendable to other building blocks.

  4. Si Nanopores Development for External Control of Transport of Biomolecules

    Energy Technology Data Exchange (ETDEWEB)

    Ileri, N; Tringe, J; Letant, S; Palozoglu, A; Stroeve, P; Faller, R


    Nazar Ileri has been involved in an independent, multidisciplinary effort to create a new class of molecular sieves for proteins and viruses. Her experimental work has been performed concurrently at two campuses, LLNL and UC Davis, while theoretical components have been largely accomplished at UC Davis. As will be described, the devices she is creating have great potential to improve very significantly the efficiency and selectivity of molecular transport over what is presently available from state-of-the-art membranes. Our biotechnology training program is based on an integrated study of the transport of biomolecules through conically-shaped, nanoporous silicon membranes. The overall objective of this effort is to demonstrate an efficient, highly selective membrane technology that is manufacturable for macroscopic areas and can be employed in sensing, diagnostic and biomedical applications. Our specific aims are to (1) fabricate and characterize the physical characteristics of the membranes, (2) to demonstrate their utility for molecular transport and separation, and (3) to develop models that will facilitate understanding of these devices as well as improved performance of the next generation of devices. We have proposed that the conical pores have superior performance characteristics compared to other porous filters. To study this hypothesis, complementary approaches from different disciplines, such as membrane synthesis, experiment, and molecular simulation need to be combined. This provides an ideal training environment for a future leader in biotechnology. Hence, for this study, Nazar Ileri has started to carry out a full range of experimental and theoretical investigations under our guidance. First, she has begun fabrication of filters with conical/pyramidal pores. She characterized the pores by AFM and SEM, and analyzed the images using wavelets and other mathematical tools. She has also started to conduct biomolecule transport experiments to compare the

  5. Radiation physics and chemistry of biomolecules. Recent developments (United States)

    Spotheim-Maurizot, Melanie


    A chapter of the book ;Radiation chemistry. From basics to application in materials and life sciences (EDP Science, Paris, France, 2008); was devoted to the state-of-the-art in the research on ionizing radiation (IR) effects on biomolecules. An update, eight years later, seemed pertinent enough to the editors of this journal who accepted to dedicate a Special Issue to the latest developments in this area of high interest for cancer radiotherapy, nuclear workers' radioprotection and food radiosterilisation. We sincerely thank them and the authors who accepted to present reviews of their most recent work. Obviously, only a small part of the research in the fascinating domain of molecular radiobiology can be covered here. Some articles are presenting the contribution of biophysical models and computational techniques to the understanding of IR effects on molecules such as DNA and proteins, or on larger systems such as chromatin, chromosomes and even cells (Nikjoo et al., Štěpán & Davídková, Ballarini & Carante, and Nikitaki et al.). In these papers, as well as in many others, several qualities of IR are compared in order to explain the observed differences of effects. The damages induced by the low energy electrons and new techniques involved in their study are discussed in great detail (Sanche and Fromm & Boulanouar). The chemistry behind the IR induced damages (single or clustered), studied in many laboratories around the world is presented in several papers (Cadet & Wagner, Sevilla et al., Chatgilialoglu et al., and Greenberg). One of them addresses a very useful comparison between the effects of IR and UV exposure on DNA (Ravanat & Douki). The majority of the papers in this Special Issue is dealing with DNA and this reflects the real situation: damages of DNA are more studied than those of other biomolecules. This is due to the role of DNA as main support of hereditary information. Nevertheless, more and more studies are outlining the influence of epigenetic

  6. 某型飞机前起落架顶起装置设计和实现%Design and Implementation of Nose Landing Gear Jack-up Equipment of an Aircraft

    Institute of Scientific and Technical Information of China (English)



    The article introduces an aircraft nose landing gear jack-up equipment which is used for simulating an aircraft's nose landing gear swerving test. The main scheme is introduced and the equipment is driven by air. The jack-up actuator is designed as body with three supports and road wheels, this kind of design can not only provide the enough strength and stiffness but also be moved easily. The electrical control section controls the magnetic exchange valve to supply air for the double-acting cylinder, this can achieve the actuator up and down. The equipment is sutably designed with not too much structures and it can be easily maintained, operated and moved, it also has breakdown protection, limit protection and urgency outage protection.%该文介绍了为配合模拟某型飞机的前轮转弯试验设计的前起落架顶起装置,给出了其总体方案。装置采用气源驱动,顶起执行机构采用三角支撑架体并配有行走轮,保证机构强度和刚度的同时又便于移动,电气控制部分控制电磁换向阀向双作用气缸供气,实现顶起机构的顶起和放下。整个装置结构紧凑、布局合理、维护简单、操作方便、移动灵活,并有故障保护、限位保护和紧急断电保护功能。

  7. The Analysis About Design of Military Training Method in the Equipment Test Base%装备试验基地军事训练方法的设计分析

    Institute of Scientific and Technical Information of China (English)

    胡利民; 宋令帮; 吴利行


    为推进装备试验基地军事训练方法设计能力,通过分析军事训练系统运行规律,依据训练要素间相互作用关系,构建了分别对应组训者、受训者、训练内容和训练环境的军事训练方法设计体系结构,并针对装备试验基地军事训练实际特点,具体设计了“三层”军事训练组训模式、“三段”军事训练受训形式、“三类”军事训练教学样式和“四化”军事训练手段方式,为提高新形势下装备试验基地军事训练效益提供借鉴.%In order to push forward the ability of military training method design for the equipment test base, the paper analyzes the interaction relationship among main factors of the military training process, and based on the relationship it defines and establishes the makeup of design system of the military training method, which is separately corresponding to the trainer, the accepter, the content, and the circumstance of military training. And contraposing to the military training characteristics of the equipment test base, it designs "three layers" pattern of organizing military training, "three phases" form of accepting military training, "three types" manner of teaching in military training, and "four turnings" mode of means in military training concretely, which afford future reference for military training benefit of the equipment test base in new times.

  8. Non-conventional Indoor GIS Layout Design of 220kV Distribution Equipment in Guangzhou Xintang Power Plant%广州新塘电厂220 kV屋内GIS非常规布置的设计

    Institute of Scientific and Technical Information of China (English)



    ∶针对广州新塘电厂2×330MW机组工程厂区布置的特殊性及局限性,文章详细分析论述了220kV配电装置采用屋内GIS型式非常规布置的设计过程及其特点,提出合适的布置方案,解决了220kV配电装置占地及出线问题,为类似工程提供借鉴。%The design and the characteristics of the non-conventional indoor GIS layout of 220kV distribution equip-ment were discussed and analyzed in the paper based on the particularity and limitation of plant layout of Guangzhou Xintang power plant, and a reasonable layout was proposed which solves the land occupation and outgoing line prob-lems of the 220kV distribution equipment and can be well referenced by similar projects.

  9. 雷达终端设备键盘鼠标通用接口板的设计与实现%Design and realization of common interface board for keyboard and mouse of radar terminal equipment

    Institute of Scientific and Technical Information of China (English)

    莫兰宾; 黄少锋


      Aiming at the problem that the general keyboard and mouse have weak generality in radar terminal equipment,a common interface board for keyboard and mouse was designed. It can realize the application of the general keyboard and mouse in radar teminal equipment successfully,reduce the use of special keyboard and mouse,save much cost and improve the sup⁃port ability of radar terminal equipment. It satisfy the requirement of army.%  针对当前普通键盘鼠标在雷达终端录取设备中通用性不强的问题,设计了键盘鼠标通用接口板,成功实现了将普通键盘鼠标运用于雷达录取终端,减少了专用键盘鼠标的使用,节约了大量成本,提高了雷达终端录取设备的保障能力,满足了部队的需求。

  10. 加油站埋地储油罐清洗设备的研究设计%Study and Design of Cleaning Equipment for Underground Oil Tank in Gas Station

    Institute of Scientific and Technical Information of China (English)

    葛汉林; 姜芳; 吴明; 胡志勇


    In this paper,according to the struc-tural characteristics of the underground oil tank in gas station,through analysis and comparison of tank cleaning technology both the domestic and o-verseas,identified the low pressure jet cleaning as overall program of cleaning equipment for under-ground oil tank in gas station.Introduced the com-position,working principle and processes of the cleaning equipment.The low pressure j et system, vacuum pumping system and electrical control sys-tem of the cleaning equipment have been designed.%针对加油站埋地储油罐的结构特点,通过对国内外油罐清洗技术的分析比较,确定低压水射流清洗作为加油站埋地储油罐清洗设备的总体方案。介绍了埋地储油罐清洗设备的功能组成、工作原理和作业流程。设计了该清洗设备的低压水射流系统、真空抽水系统和电气控制系统。

  11. 基于虚拟技术的矿山设备维修训练系统设计研究%Design of maintenance training system for mining equipments based on virtualization technology

    Institute of Scientific and Technical Information of China (English)



    以改进矿山设备维修训练手段为目的,通过对矿山设备虚拟维修系统开发关键技术的分析,提出了基于虚拟技术的矿山设备维修训练系统结构设计思路;并将建立好的设备训练系统应用于矿山维修训练中,达到了降低训练费用、缩短训练时间及提高训练效果的目的.%In order to improve the maintenance training methods for mining equipments, after analysing key technologies of developing virtual maintenance system for mining equipments, the ideas of designing the structure of maintenance training system for mining equipments based on virtualization technology are proposed.In addition, the established maintenance training system is applied to maintenance training in a mine, thus training costs decrease, training duration shortens and training effects improve.

  12. 面向服务的装备远程测试与故障诊断系统的设计%Design of Remote Test and Fault Diagnosis System for Service-Oriented Equipment

    Institute of Scientific and Technical Information of China (English)

    汤宫民; 刘福军; 尹晓虎; 汤潇奕; 梁清果


    In view of the requirements of the new equipment maintenance support tecnoiogy,and combining with the characteristics of campaign-level equipment maintenance support organization, the article relies on the resourses of the existing test and fault diagnosis system for campaign-level equipment maintenance support organization. On the basis of demand analysis of equipment remote test and fault diagnosis system, and using the service-oriented architecture thought, the article designs the software architecture and hardware structure of service-oriented remote testing and fault diagnosis system, and analyzes the all business flow layer functions, field test side, communication network and remote technical support center side. The scalability & extensibility of equipment performance test and maintenance fault diagnosis system for campaign-level maintenance support organization are increased, and the real-time performances of remote control and data analysis are improved. And the system can realize the remote control, resourses sharing and information fusion in the true sense, and benefits to respond flexibly the maintenance demand changes for campaign-level equipment maintenance support organization.%针对新型装备维修技术保障需求,本文结合战役级装备维修保障机构特点,依托战役级装备维修机构现有测试和故障诊断系统资源,在对装备远程测试与故障诊断系统需求分析的基础上,利用面向服务的体系架构思想,设计了面向服务的广域分布式远程测试与故障诊断系统软件架构和硬件结构,并对其各业务流层功能和现场测试端、通信网络、远程技术支援中心端进行了分析。对于提高战役级装备性能测试和维修故障诊断系统的伸缩性和扩展性,提高远程控制、数据分析的实时性能,实现真正意义上的远程控制、资源共享和信息融合,有利于修理机构灵活地应对维修需求的变化。

  13. Armature Guide Ring Automation Assembly Equipment Feeding Mechanism Design Based on TRIZ%基于 TRIZ 的导向环自动装配设备上料机构设计

    Institute of Scientific and Technical Information of China (English)

    周为; 王剑彬; 向友


    针对雨刮器电机自动化生产线配套设备,电枢导向环自动装配设备结构设计中如何高效、稳定供料的问题,提出运用T RIZ创新设计方法中的冲突解决原理进行电枢导向环自动装配设备的上料机构设计。通过分析上料机构功能设计需求与T RIZ中39个工程参数的关系,建立上料机构的矛盾矩阵,用冲突解决原理进行分析,找到相应的原理解,结合传统设计经验提出设计方案并评估,最终确定了电枢导向环自动装配设备上料机构的设计方案。%To design an efficient and stable armature guide ring automation assembly equipment in wiper motor automation production line ,the paper put forward using the conflict resolution principle of TRIZ to design the feeding mechanism .Through the analysis on the relationship of the functional design requirements and 39 engineering parameters in TRIZ ,the contradictions matrix of feeding mechanism was set up ,and analyzed by the conflict resolution principle ,the corresponding original solution was found .Combined with traditional design experience ,the design scheme was put forward and evaluated ,eventually the armature guide ring automation assembly equipment feeding mechanism was designed .

  14. “环保设备原理与设计”实践环节的构建与实践%Construction and practice of practical links of Principle & Design for Environmental Protection Equipment course

    Institute of Scientific and Technical Information of China (English)

    周翠红; 陈家庆; 常俊英


    “环保设备原理与设计”是我校的核心课程,为进一步突出环保设备与工程实践特色的人才培养理念,新开设“环保设备原理与设计课程设计”与“环保设备拆装与测绘”2个实践环节.通过实验室建设、教师培训、指导书的编写等创造了课程开设条件.实施过程中注重与相关教学环节的融合,精心选择题目,重视计算机辅助设计(AutoCAD、UG NX)与多媒体的使用,增强了学生的工程实践能力与动手能力.将在优化学生分组与注重过程考核等方面继续努力,以达到提高实践教学水平的目的.%The Principle and Design for Environmental Protection Equipment is a major course in our institute. In order to improve the cultivation ideas of environmental protection equipment and engineering practice, two practical education courses named "Principle &. Design for Environmental Protection Equipment" and "Disassembly and Mapping of Environmental Protection Equipment" are set up. By laboratory construction, teacher training, preparation of guide books, etc. , their setting up conditions are created. When two courses are opening, this article focuses on the integration of other course, carefully selected topics of disassembly and design, strengthened computer aided design (including AutoCAD, UG NX) and multimedia use application. Now there are some achievements of improving students' practice ability. It will optimize topic group and pay more attention to process-oriented assessment to improve practical educational system effectiveness.

  15. Conductive Paper with Antibody-Like Film for Electrical Readings of Biomolecules (United States)

    Tavares, Ana P. M.; Ferreira, Nádia S.; Truta, Liliana A. A. N. A.; Sales, M. Goreti F.


    This work reports a novel way of producing an inexpensive substrate support to assemble a sensing film, designed for the electrical transduction of an intended biomolecule. The support uses cellulose paper as substrate, made hydrophobic with solid wax and covered by a home-made conductive ink having graphite as core material. The hydrophobicity of the paper was confirmed by contact angle measurements and the conductive ink composition was optimized with regard to its adhesion, conductivity, and thermal stability. This support was further modified targeting its application in quantitative analysis. Carnitine (CRT) was selected as target compound, a cancer biomarker. The recognition material consisted of an antibody-like receptor film for CRT, tailored on the support and prepared by electrically-sustained polymerization of 3,4-ethylenedioxythiophene (EDOT) or dodecylbenzenesulfonic acid (DBS). Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy analysis confirmed the presence of the polymeric film on the support, and the performance of the devices was extensively evaluated with regard to linear response ranges, selectivity, applicability, and reusability. Overall, the paper-based sensors offer simplicity of fabrication, low cost and excellent reusability features. The design could also be extended to other applications in electrical-based approaches to be used in point-of-care (POC).

  16. Design of supply and demand platform of equipment manufacturing industry based on cloud computing%基于云计算的装备制造业供求平台设计∗

    Institute of Scientific and Technical Information of China (English)

    赵红; 苏剑峰


    随着信息技术的快速发展,我国装备制造企业需要加快信息化建设的步伐,并进行技术升级和资源整合。云计算作为一种新兴的数据处理模式,能够加强装备制造企业之间的信息共享。在分析装备制造业对产品、技术、设备和服务等需求的基础上,提出基于云计算的平台架构方案。对平台功能模块进行描述,设计出基于云计算技术的平台业务流程数据模型,为进一步实现系统目标和满足客户需求提供借鉴。%With the rapid development of information technology, the enterprises of equipment manufacturing in China need to accelerate the pace of information construction, and upgrade the technology and integrate the resources. As a new data processing mode, cloud computing can strengthen the information sharing between the equipment manufacturing enterprises. Based on the analysis of the requirements of products, technology, equipment and services in equipment manufacturing industry, the framework of the platform is proposed based on cloud computing. The platform function module is described, the business process data platform model is designed based on cloud calculation technology, in order to provide reference for realizing the system goal and further meeting customer demand.

  17. Designing Program for Multi-function Optical Fiber and Laser Machining Equipment%光纤激光多功能加工设备设计方案

    Institute of Scientific and Technical Information of China (English)

    吴国良; 李建伟; 郑志强; 曹巍巍


    该设备的方案为选配光纤传导的大功率光纤激光器,将聚焦系统连接在机器人手腕上,集成为柔性激光加工设备,运动的执行机构为激光束扫描运动的机器人+工件旋转的转台系统,可以实现各种形状零件的全自动激光加工。%The program is to select a large-powered optical fiber laser for optical fiber transmitting. It connects the focusing system to the wrist of a robot, integrated to be a flexible laser machining equipment, the manipulators are laser beam scanning robot and workpiece rotating floor, which can realize fully automatic laser machining of various-shape parts.

  18. 2007 special equipment safety

    Institute of Scientific and Technical Information of China (English)


    @@ The General Administration of Quality Supervision, Inspection and Quarantine of P.R.China (AQSIQ) issued a notice on May 28, 2007,requiring various locations to rectify their procedures for checking special equipment and hoisting machines for hidden problems. To further clarify and implement responsibility in the safety management of special equipment in enterprises, inspection responsibilities and test organizations related to technical assurance are to be established. Further, quality inspection departments will be supervised by law in order to improve special equipment safety.

  19. Waste Handling Equipment Devleopment Test and Evaluation

    Energy Technology Data Exchange (ETDEWEB)

    R.L. Tome


    The purpose of this study is to identify candidate Monitored Geologic Repository (MGR) surface waste handling equipment for development testing. This study will also identify strategies for performing the development tests. Development testing shall be implemented to support detail design and reduce design risks. Development testing shall be conducted to confirm design concepts, evaluate alternative design concepts, show the availability of needed technology, and provide design documentation. The candidate equipment will be selected from MGR surface waste handling equipment that is the responsibility of the Management and Operating Contractor (M&O) Surface Design Department. The equipment identified in this study is based on Viability Assessment (VA) design. The ''Monitored Geologic Repository Test and Evaluation Plan'' (MGR T&EP), Reference 5.1, was used as a basis for this study. The MGR T&EP reflects the extent of test planning and analysis that can be conducted, given the current status of the MGR requirements and latest VA design information. The MGR T&EP supports the appropriate sections in the license application (LA) in accordance with 10 CFR 60.2 1(c)(14). The MGR T&EP describes the following test activities: site characterization to confirm, by test and analysis, the suitability of the Yucca Mountain site for housing a geologic repository; development testing to investigate and document design concepts to reduce risk; qualification testing to verify equipment compliance with design requirements, specifications, and regulatory requirements; system testing to validate compliance with MGR requirements, which include the receipt, handling, retrieval, and disposal of waste; periodic performance testing to verify preclosure requirements and to demonstrate safe and reliable MGR operation; and performance confirmation modeling, testing, and analysis to verify adherence to postclosure regulatory requirements. Development test activities can be

  20. Tailoring GaN semiconductor surfaces with biomolecules. (United States)

    Estephan, Elias; Larroque, Christian; Cuisinier, Frédéric J G; Bálint, Zoltán; Gergely, Csilla


    Functionalization of semiconductors constitutes a crucial step in using these materials for various electronic, photonic, biomedical, and sensing applications. Within the various possible approaches, selection of material-binding biomolecules from a random biological library, based on the natural recognition of proteins or peptides toward specific material, offers many advantages, most notably biocompatibility. Here we report on the selective functionalization of GaN, an important semiconductor that has found broad uses in the past decade due to its efficient electroluminescence and pronounced chemical stability. A 12-mer peptide ("GaN_probe") with specific recognition for GaN has evolved. The subtle interplay of mostly nonpolar hydrophobic and some polar amino acidic residues defines the high affinity adhesion properties of the peptide. The interaction forces between the peptide and GaN are quantified, and the hydrophobic domain of the GaN_probe is identified as primordial for the binding specificity. These nanosized binding blocks are further used for controlled placement of biotin-streptavidin complexes on the GaN surface. Thus, the controlled grow of a new, patterned inorganic-organic hybrid material is achieved. Tailoring of GaN by biological molecules can lead to a new class of nanostructured semiconductor-based devices.

  1. Polyacrylamide medium for the electrophoretic separation of biomolecules (United States)

    Madabhushi, Ramakrishna S.; Gammon, Stuart A.


    A polyacryalmide medium for the electrophoretic separation of biomolecules. The polyacryalmide medium comprises high molecular weight polyacrylamides (PAAm) having a viscosity average molecular weight (M.sub.v) of about 675-725 kDa were synthesized by conventional red-ox polymerization technique. Using this separation medium, capillary electrophoresis of BigDye DNA sequencing standard was performed. A single base resolution of .about.725 bases was achieved in .about.60 minute in a non-covalently coated capillary of 50 .mu.m i.d., 40 cm effective length, and a filed of 160 V/cm at C. The resolution achieved with this formulation to separate DNA under identical conditions is much superior (725 bases vs. 625 bases) and faster (60 min. vs. 75 min.) to the commercially available PAAm, such as supplied by Amersham. The formulation method employed here to synthesize PAAm is straight-forward, simple and does not require cumbersome methods such as emulsion polymerizaiton in order to achieve very high molecular weights. Also, the formulation here does not require separation of PAAm from the reaction mixture prior to reconstituting the polymer to a final concentration. Furthermore, the formulation here is prepared from a single average mol. wt. PAAm as opposed to the mixture of two different average mo. wt. PAAm previously required to achieve high resolution.

  2. Interaction mechanism of biomolecules on vacancy defected 2D materials (United States)

    Gürel, Hikmet Hakan; Salmankurt, Bahadır


    In this work, we present a first principles study of the adsorption of Adenine which is a nucleobases, Histide and Leucine molecules, which are the amino acids, on vacancy defected single layer materials such as graphene and phosphorene. Among these materials, graphene, which is a single layer honeycomb structure of carbon. Also, phosphorene is recently synthesized by mechanical exfoliation of the black phosphorus. Phosphorene forming a puckered honeycomb structure similar to silicene. However, unlike zero-bandgap graphene and silicene, phosphorene is a direct band gap semiconductor, which makes it very attractive for the nanoelectronic devices. According to the studies, local defects can always exist at any temperature. The most probable defect type is the single vacancy in the single layer honeycomb structures. Vacancy defects can be emerged during growth process and they change the properties of materials significantly. In this study, we show that how to manipulate interaction and binding mechanisms of biomolecules with 2D materials with increased chemical activity by vacancy defects.

  3. Regioselective Localization and Tracking of Biomolecules on Single Gold Nanoparticles. (United States)

    Rajeeva, Bharath Bangalore; Hernandez, Derek S; Wang, Mingsong; Perillo, Evan; Lin, Linhan; Scarabelli, Leonardo; Pingali, Bharadwaj; Liz-Marzán, Luis M; Dunn, Andrew K; Shear, Jason B; Zheng, Yuebing


    Selective localization of biomolecules at the hot spots of a plasmonic nanoparticle is an attractive strategy to exploit the light-matter interaction due to the high field concentration. Current approaches for hot spot targeting are time-consuming and involve prior knowledge of the hot spots. Multiphoton plasmonic lithography is employed to rapidly immobilize bovine serum albumin (BSA) hydrogel at the hot spot tips of a single gold nanotriangle (AuNT). Regioselectivity and quantity control by manipulating the polarization and intensity of the incident laser are also established. Single AuNTs are tracked using dark-field scattering spectroscopy and scanning electron microscopy to characterize the regioselective process. Fluorescence lifetime measurements further confirm BSA immobilization on the AuNTs. Here, the AuNT-BSA hydrogel complexes, in conjunction with single-particle optical monitoring, can act as a framework for understanding light-molecule interactions at the subnanoparticle level and has potential applications in biophotonics, nanomedicine, and life sciences.

  4. Quantification of specific bindings of biomolecules by magnetorelaxometry

    Directory of Open Access Journals (Sweden)

    Steinhoff Uwe


    Full Text Available Abstract The binding reaction of the biomolecules streptavidin and anti-biotin antibody, both labelled by magnetic nanoparticles (MNP, to biotin coated on agarose beads, was quantified by magnetorelaxometry (MRX. Highly sensitive SQUID-based MRX revealed the immobilization of the MNP caused by the biotin-streptavidin coupling. We found that about 85% of streptavidin-functionalised MNP bound specifically to biotin-agarose beads. On the other hand only 20% of antibiotin-antibody functionalised MNP were specifically bound. Variation of the suspension medium revealed in comparison to phosphate buffer with 0.1% bovine serum albumin a slight change of the binding behaviour in human serum, probably due to the presence of functioning (non heated serum proteins. Furthermore, in human serum an additional non-specific binding occurs, being independent from the serum protein functionality. The presented homogeneous bead based assay is applicable in simple, uncoated vials and it enables the assessment of the binding kinetics in a volume without liquid flow. The estimated association rate constant for the MNP-labelled streptavidin is by about two orders of magnitude smaller than the value reported for free streptavidin. This is probably due to the relatively large size of the magnetic markers which reduces the diffusion of streptavidin. Furthermore, long time non-exponential kinetics were observed and interpreted as agglutination of the agarose beads.

  5. Microfluidic Droplet Dehydration for Concentrating Processes in Biomolecules (United States)

    Anna, Shelley


    Droplets in microfluidic devices have proven useful as picoliter reactors for biochemical processing operations such as polymerase chain reaction, protein crystallization, and the study of enzyme kinetics. Although droplets are typically considered to be self-contained, constant volume reactors, there can be significant transport between the dispersed and continuous phases depending on solubility and other factors. In the present talk, we show that water droplets trapped within a microfluidic device for tens of hours slowly dehydrate, concentrating the contents encapsulated within. We use this slow dehydration along with control of the initial droplet composition to influence gellation, crystallization, and phase separation processes. By examining these concentrating processes in many trapped drops at once we gain insight into the stochastic nature of the events. In one example, we show that dehydration rate impacts the probability of forming a specific crystal habit in a crystallizing amino acid. In another example, we phase separate a common aqueous two-phase system within droplets and use the ensuing two phases to separate DNA from an initial mixture. We further influence wetting conditions between the two aqueous polymer phases and the continuous oil, promoting complete de-wetting and physical separation of the polymer phases. Thus, controlled dehydration of droplets allows for concentration, separation, and purification of important biomolecules on a chip.

  6. Diatom-based label-free optical biosensor for biomolecules. (United States)

    Viji, S; Anbazhagi, M; Ponpandian, N; Mangalaraj, D; Jeyanthi, S; Santhanam, P; Devi, A Shenbaga; Viswanathan, C


    Diatoms are unicellular algae, which fabricates ornate biosilica shells called frustules that possess a surface rich in reactive silanol (Si-OH) groups. The intrinsic patterned porous structure of diatom frustules at nanoscale can be exploited in the effective detection of biomolecules. In this study, the frustules of a specific diatom Amphora sp. has been functionalized to detect bovine serum albumin (BSA). The functionalization of the diatom frustule substrate is achieved by using 3-aminopropyltriethoxysilane (APES). The field emission scanning electron microscopy (FESEM) results showed an ornately patterned surface of the frustule valve ordered at nanoscale. The Fourier transform infrared (FTIR) spectra confirmed the N-H bending and stretching of the amine group after amine functionalization. The emission peaks in the photoluminescence (PL) spectra of the amine-functionalized diatom biosilica selectively enhanced the intensity by a factor of ten when compared to that of a bare diatom biosilica. The result showed a significant quenching of PL intensity of BSA at around 445 nm due to the interaction of amine-functionalized diatom-BSA protein complex. The detection limit was found to be 3 × 10(-5) M of BSA protein. Hence, the study proves that the functionalized frustule of Amphora sp. is an effective quantitative analytical tool for optical label-free biosensing applications.

  7. Small biomolecule immunosensing with plasmonic optical fiber grating sensor. (United States)

    Ribaut, Clotilde; Voisin, Valérie; Malachovská, Viera; Dubois, Valentin; Mégret, Patrice; Wattiez, Ruddy; Caucheteur, Christophe


    This study reports on the development of a surface plasmon resonance (SPR) optical fiber biosensor based on tilted fiber Bragg grating technology for direct detection of small biomarkers of interest for lung cancer diagnosis. Since SPR principle relies on the refractive index modifications to sensitively detect mass changes at the gold coated surface, we have proposed here a comparative study in relation to the target size. Two cytokeratin 7 (CK7) samples with a molecular weight ranging from 78 kDa to 2.6 kDa, respectively CK7 full protein and CK7 peptide, have been used for label-free monitoring. This work has first consisted in the elaboration and the characterization of a robust and reproducible bioreceptor, based on antibody/antigen cross-linking. Immobilized antibodies were then utilized as binding agents to investigate the sensitivity of the biosensor towards the two CK7 antigens. Results have highlighted a very good sensitivity of the biosensor response for both samples diluted in phosphate buffer with a higher limit of detection for the larger CK7 full protein. The most groundbreaking nature of this study relies on the detection of small biomolecule CK7 peptides in buffer and in the presence of complex media such as serum, achieving a limit of detection of 0.4 nM.

  8. Electron cross-sections and transport in liquids and biomolecules (United States)

    White, Ronald; Casey, M.; Cocks, D.; Konvalov, D.; Brunger, M. J.; Garcia, G.; Petrovic, Z.; McEachran, R.; Buckman, S. J.; de Urquijo, J.


    Modelling of electron induced processes in plasma medicine and radiation damage is reliant on accurate self-consistent sets of cross-sections for electrons in tissue. These cross-sections (and associated transport theory) must accurately account not only the electron-biomolecule interactions but also for the soft-condensed nature of tissue. In this presentation, we report on recent swarm experiments for electrons in gaseous water and tetrahydrofuran using the pulsed-Townsend experiment, and the associated development of self-consistent cross-section sets that arise from them. We also report on the necessary modifications to gas-phase cross-sections required to accurately treat electron transport in liquids. These modifications involve the treatment of coherent scattering and screening of the electron interaction potential as well as the development of a new transport theory to accommodate these cross-sections. The accuracy of the ab-initio cross-sections is highlighted through comparison of theory and experiment for electrons in liquid argon and xenon.

  9. Two dimensional electron spin resonance: Structure and dynamics of biomolecules (United States)

    Saxena, Sunil; Freed, Jack H.


    The potential of two dimensional (2D) electron spin resonance (ESR) for measuring the structural properties and slow dynamics of labeled biomolecules will be presented. Specifically, it will be shown how the recently developed method of double quantum (DQ) 2D ESR (S. Saxena and J. H. Freed, J. Chem. Phys. 107), 1317, (1997) can be used to measure large interelectron distances in bilabeled peptides. The need for DQ ESR spectroscopy, as well as the challenges and advantages of this method will be discussed. The elucidation of the slow reorientational dynamics of this peptide (S. Saxena and J. H. Freed, J. Phys. Chem. A, 101) 7998 (1997) in a glassy medium using COSY and 2D ELDOR ESR spectroscopy will be demonstrated. The contributions to the homogeneous relaxation time, T_2, from the overall and/or internal rotations of the nitroxide can be distinguished from the COSY spectrum. The growth of spectral diffusion cross-peaks^2 with mixing time in the 2D ELDOR spectra can be used to directly determine a correlation time from the experiment which can be related to the rotational correlation time.

  10. Radiation damage of biomolecules (RADAM) database development: current status (United States)

    Denifl, S.; Garcia, G.; Huber, B. A.; Marinković, B. P.; Mason, N.; Postler, J.; Rabus, H.; Rixon, G.; Solov'yov, A. V.; Suraud, E.; Yakubovich, A. V.


    Ion beam therapy offers the possibility of excellent dose localization for treatment of malignant tumours, minimizing radiation damage in normal tissue, while maximizing cell killing within the tumour. However, as the underlying dependent physical, chemical and biological processes are too complex to treat them on a purely analytical level, most of our current and future understanding will rely on computer simulations, based on mathematical equations, algorithms and last, but not least, on the available atomic and molecular data. The viability of the simulated output and the success of any computer simulation will be determined by these data, which are treated as the input variables in each computer simulation performed. The radiation research community lacks a complete database for the cross sections of all the different processes involved in ion beam induced damage: ionization and excitation cross sections for ions with liquid water and biological molecules, all the possible electron - medium interactions, dielectric response data, electron attachment to biomolecules etc. In this paper we discuss current progress in the creation of such a database, outline the roadmap of the project and review plans for the exploitation of such a database in future simulations.

  11. Hybrid carbon nanomaterials for electrochemical detection of biomolecules (United States)

    Laurila, Tomi


    Electrochemical detection of different biomolecules in vivo is a promising path towards in situ monitoring of human body and its functions. However, there are several major obstacles, such as sensitivity, selectivity and biocompatiblity, which must be tackled in order to achieve reliably and safely operating sensor devices. Here we show that by utilizing hybrid carbon materials as electrodes to detect two types of neurotransmitters, dopamine and glutamate, several advantages over commonly used electrode materials can be achieved. In particular, we will demonstrate here that it is possible to combine the properties of different carbon allotropes to obtain hybrid materials with greatly improved electrochemical performance. Three following examples of the approach are given: (i) diamond-like carbon (DLC) thin film electrodes with different layer thicknesses, (ii) multi-walled carbon nanotubes grown directly on top of DLC and (iii) carbon nanofibres synthesized on top of DLC thin films. Detailed structural and electrochemical characterization is carried out to rationalize the reasons behind the observed behvior. In addition, results from the atomistic simulations are utilized to obtain more information about the properties of the amorphous carbon thin films.

  12. Silver cluster-biomolecule hybrids: from basics towards sensors. (United States)

    Bonačić-Koutecký, Vlasta; Kulesza, Alexander; Gell, Lars; Mitrić, Roland; Antoine, Rodolphe; Bertorelle, Franck; Hamouda, Ramzi; Rayane, Driss; Broyer, Michel; Tabarin, Thibault; Dugourd, Philippe


    We focus on the functional role of small silver clusters in model hybrid systems involving peptides in the context of a new generation of nanostructured materials for biosensing. The optical properties of hybrids in the gas phase and at support will be addressed with the aim to bridge fundamental and application aspects. We show that extension and enhancement of absorption of peptides can be achieved by small silver clusters due to the interaction of intense intracluster excitations with the π-π* excitations of chromophoric aminoacids. Moreover, we demonstrate that the binding of a peptide to a supported silver cluster can be detected by the optical fingerprint. This illustrates that supported silver clusters can serve as building blocks for biosensing materials. Moreover, the clusters can be used simultaneously to immobilize biomolecules and to increase the sensitivity of detection, thus replacing the standard use of organic dyes and providing label-free detection. Complementary to that, we show that protected silver clusters containing a cluster core and a shell liganded by thiolates exhibit absorption properties with intense transitions in the visible regime which are also suitable for biosensing applications.

  13. Medical equipment management

    CERN Document Server

    Willson, Keith; Tabakov, Slavik


    Know What to Expect When Managing Medical Equipment and Healthcare Technology in Your Organization As medical technology in clinical care becomes more complex, clinical professionals and support staff must know how to keep patients safe and equipment working in the clinical environment. Accessible to all healthcare professionals and managers, Medical Equipment Management presents an integrated approach to managing medical equipment in healthcare organizations. The book explains the underlying principles and requirements and raises awareness of what needs to be done and what questions to ask. I

  14. Exercise and sports equipment: some ergonomics aspects. (United States)

    Reilly, T; Lees, A


    Sports equipment encompasses a gamut of devices used in laboratory, training and competitive contexts and these form the content of this paper. Ergometers range in sophistication from friction braked stationary bicycles to computer controlled simulators which incorporate exercise modes specific to the athletic user. These are now used in training, as experimental devices and in some instances for competition purposes. Training equipment exhibits a similar emphasis on exercise specificity, safety being an important aspect of its use. Design of projectiles for sporting activities has mainly reflected their traditional modes of use, the introduction of synthetic materials having some ergonomics implications. Similarly, materials science and design technology have contributed innovations in equipment for racquet sports and hitting implements. The changes have tended to be associated with availability of new materials for product construction and have implications for safety and skill in the transition to using the new products. Ski equipment design illustrates ergonomics factors in interfacing the performer with the sporting environment and how equipment has progressed by regenerative design processes. Enhancement of performance in some sports must be accompanied by an awareness of safety requirements: where appropriate, risks to participants should be reduced by use of protective clothing and equipment. Enforced validation of protective equipment is recommended to raise safety levels in certain sports and the safety of spectators must not be neglected. Human factors criteria can then be applied in monitoring, officiating and spectating at sporting events.

  15. Morphological changes of calcite single crystals induced by graphene-biomolecule adducts (United States)

    Calvaresi, Matteo; Di Giosia, Matteo; Ianiro, Alessandro; Valle, Francesco; Fermani, Simona; Polishchuk, Iryna; Pokroy, Boaz; Falini, Giuseppe


    Calcite has the capability to interact with a wide variety of molecules. This usually induces changes in shape and morphology of crystals. Here, this process was investigated using sheets of graphene-biomolecule adducts. They were prepared and made dispersible in water through the exfoliation of graphite by tip sonication in the presence tryptophan or N-acetyl-D-glucosamine. The crystallization of calcium carbonate in the presence of these additives was obtained by the vapor diffusion method and only calcite formed. The analysis of the microscopic observations showed that the graphene-biomolecule adducts affected shape and morphology of rhombohedral {10.4} faced calcite crystals, due to their stabilization of additional {hk.0} faces. The only presence of the biomolecule affected minimally shape and morphology of calcite crystals, highlighting the key role of the graphene sheets as 2D support for the adsorption of the biomolecules.

  16. Toward the Atomic-Level Mass Analysis of Biomolecules by the Scanning Atom Probe. (United States)

    Nishikawa, Osamu; Taniguchi, Masahiro


    In 1994, a new type of atom probe instrument, named the scanning atom probe (SAP), was proposed. The unique feature of the SAP is the introduction of a small extraction electrode, which scans over a specimen surface and confines the high field, required for field evaporation of surface atoms in a small space, between the specimen and the electrode. Thus, the SAP does not require a sharp specimen tip. This indicates that the SAP can mass analyze the specimens which are difficult to form in a sharp tip, such as organic materials and biomolecules. Clean single wall carbon nanotubes (CNT), made by high-pressure carbon monoxide process are found to be the best substrates for biomolecules. Various amino acids and dipeptide biomolecules were successfully mass analyzed, revealing characteristic clusters formed by strongly bound atoms in the specimens. The mass analysis indicates that SAP analysis of biomolecules is not only qualitative, but also quantitative.

  17. Hard X-ray-induced optical luminescence via biomolecule-directed metal clusters. (United States)

    Osakada, Yasuko; Pratx, Guillem; Sun, Conroy; Sakamoto, Masanori; Ahmad, Moiz; Volotskova, Olga; Ong, Qunxiang; Teranishi, Toshiharu; Harada, Yoshie; Xing, Lei; Cui, Bianxiao


    Here, we demonstrate that biomolecule-directed metal clusters are applicable in the study of hard X-ray excited optical luminescence, promising a new direction in the development of novel X-ray-activated imaging probes.

  18. Biomolecule Sequencer: Nanopore Sequencing Technology for In-Situ Environmental Monitoring and Astrobiology (United States)

    John, K. K.; Botkin, D. J.; Burton, A. S.; Castro-Wallace, S. L.; Chaput, J. D.; Dworkin, J. P.; Lupisella, M. L.; Mason, C. E.; Rubins, K. H.; Smith, D. J.; Stahl, S.; Switzer, C.


    Biomolecule Sequencer will demonstrate, for the first time, that DNA sequencing is feasible as a tool for in-situ environmental monitoring and astrobiology. A space-based sequencer could identify microbes, diseases, and help detect DNA-based life.

  19. Cu(I), Ag(I), Cd(II), and Pb(II) binding to biomolecules studied by perturbed angular correlation of $\\gamma$-rays (PAC) spectroscopy

    CERN Multimedia

    Metal ions display diverse functions in biological systems and are essential components in both protein and nucleic acid structure and function, and in control of biochemical reaction paths and signalling. Similarly, metal ions may be used to control structure and function of synthetic biomolecules, and thus be a tool in the design of molecules with a desired function. In this project we address a variety of questions concerning both the function of metal ions in natural systems, in synthetic biomolecules, and the toxic effect of some metal ions. All projects involve other experimental techniques such as NMR, EXAFS, UV-Vis, fluorescence, and CD spectroscopies providing complementary data, as well as interpretation of the experimental data by quantum mechanical calculations of spectroscopic properties. The isotopes to be employed in the proposal are the following: $^{111m}$Cd, $^{111}$Ag, $^{199m}$Hg, $^{204m}$Pb, $^{61}$Cu, $^{68m}$Cu

  20. Determination of the labeling density of fluorophore-biomolecule conjugates with absorption spectroscopy. (United States)

    Grabolle, Markus; Brehm, Robert; Pauli, Jutta; Dees, Franziska M; Hilger, Ingrid; Resch-Genger, Ute


    Dye-biomolecule conjugation is frequently accompanied by considerable spectral changes of the dye's absorption spectrum that limit the use of the common photometrical method for the determination of labeling densities. Here, we describe an improvement of this method using the integral absorbance of the dye instead of its absorbance at the long wavelength maximum to determine the concentration of the biomolecule-coupled dye. This approach is illustrated for three different cyanine dyes conjugated to the antibody IgG.

  1. Recovery of biomolecules from marinated herring (Clupea harengus) brine using ultrafiltration through ceramic membranes

    DEFF Research Database (Denmark)

    Gringer, Nina; Hosseini, Seyed Vali; Svendsen, Tore;


    on recovery of high value biomolecules such as proteins, fatty acids, minerals, and phenolic compounds. Chemical and biological oxygen demand (COD, BOD5) as well as total suspended solids (TSS) were also measured to follow the performance of the ultrafiltration. The retentates contained 75-82% (95% TSS...... that ceramic ultrafiltration can recover biomolecules from marinated herring brines although pre-filtration optimization is still needed....

  2. Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator. (United States)

    Chen, Chia-Hung; Sarkar, Aniruddh; Song, Yong-Ak; Miller, Miles A; Kim, Sung Jae; Griffith, Linda G; Lauffenburger, Douglas A; Han, Jongyoon


    We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (~10-fold) reduced the time required to complete the assay and the sample volume used.

  3. Plastic Trash goes Biohybrid"-Rapid and Selective Functionalization of Inert Plastic Surfaces with Biomolecules

    DEFF Research Database (Denmark)

    Schiller, Stefan M; Kambhampati, Dev; Stengel, Gudrun


    The covalent functionalization of "inert" polymers such as polypropylene with biomolecules for biocompatible or biosensor surfaces is challenging. Here we present a powerful approach to covalently modify "inert" macromolecular surfaces with biomacromolecules reusing old plastic material. A specia...... with a thin reactive plasma polymerized maleic anhydride nanolayer network, which can be subsequently modified with biomolecules for various applications, e.g., in tissue engineering and as biochips...

  4. Design of Magnesium Alloy Mixing Equipment with Frequency Control Function%具有变频调速功能的镁合金搅拌设备的设计

    Institute of Scientific and Technical Information of China (English)

    赵赛; 吴建龙; 邹文兵; 成群林; 曹雪峰; 别亚星


    In the melting process of Magnesium alloy, to realize automatic mixing for Magnesium alloy melt and to explore automatic mixing parameters, a magnesium alloy mixing equipment with speed control function is developed. The structure and working principle of the equipment and the key parts design are introduced. Using inverter and variable-frequency motor to regulate mixing speed. The design rationality is verified by experiment, and automatic mixing parameters of magnesium alloy are obtained. The equipment has simple structure, low cost, can be used to research automatic mixing process, and improves automatic level of magnesium alloy melting.%在镁合金熔炼过程中,为了实现对镁合金熔液的自动搅拌,并探索出自动搅拌工艺参数,研制了一台具有调速功能的镁合金搅拌设备。介绍了设备的结构、工作原理和关键部件的设计,采用变频器和变频电机组成变频调速系统,在控制面板上通过电位器对搅拌速度进行调节。通过试验验证了设计的合理性,并获得了镁合金自动搅拌工艺参数。该设备结构简单,成本低,可用于自动搅拌工艺的研究,提高了镁合金熔炼的自动化水平。

  5. Electric utility engineer`s FGD manual -- Volume 2: Major mechanical equipment; FGD proposal evaluations; Use of FGDPRISM in FGD system modification, proposal, evaluation, and design; FGD system case study. Final report

    Energy Technology Data Exchange (ETDEWEB)



    Part 2 of this manual provides the electric utility engineer with detailed technical information on some of the major mechanical equipment used in the FGD system. The objectives of Part 2 are the following: to provide the electric utility engineer with information on equipment that may be unfamiliar to him, including ball mills, vacuum filters, and mist eliminators; and to identify the unique technique considerations imposed by an FGD system on more familiar electric utility equipment such as fans, gas dampers, piping, valves, and pumps. Part 3 provides an overview of the recommended procedures for evaluating proposals received from FGD system vendors. The objectives are to provide procedures for evaluating the technical aspects of proposals, and to provide procedures for determining the total costs of proposals considering both initial capital costs and annual operating and maintenance costs. The primary objective of Part 4 of this manual is to provide the utility engineer who has a special interest in the capabilities of FGDPRISM [Flue Gas Desulfurization PRocess Integration and Simulation Model] with more detailed discussions of its uses, requirements, and limitations. Part 5 is a case study in using this manual in the preparation of a purchase specification and in the evaluation of proposals received from vendors. The objectives are to demonstrate how the information contained in Parts 1 and 2 can be used to improve the technical content of an FGD system purchase specification; to demonstrate how the techniques presented in Part 3 can be used to evaluate proposals received in response to the purchase specification; and to illustrate how the FGDPRISM computer program can be used to establish design parameters for the specification and evaluate vendor designs.

  6. Composting equipment design and technology for rural refuse disposal%处理农村生活垃圾装置的研制及工艺

    Institute of Scientific and Technical Information of China (English)

    文国来; 王德汉; 李俊飞; 邹璇; 孙艳军


    为了探索农村生活垃圾的处理途径,该文研究了一种新型一体化处理生活垃圾的装置及工艺,以广州市番禺区猛涌村生活垃圾为研究对象,进行了2个月的中试试验.研制的堆肥装置分4个小仓,每个小仓容纳垃圾1.56 m3,总容积6.24 m3,抽风机功率为1.1 kW/h,抽风频率为1 d 10次,每次30 min,总时间为5 h,每天总耗电量5.5 kW,能耗低.通过12 d高温发酵,24 d编织袋二次发酵的处理方式,温度能达到60℃的天数在5 d以上,出仓时减容约为40%,堆肥产品质量符合要求,且整个处理过程无二次污染,结果表明该装置及工艺可以用来处理农村生活垃圾,可充分做到垃圾减量化、无害化和资源化.%The environmental risk caused by the rural refuse increasingly is proved to be a big issue because there is not enough place for landfill. A new kind of composting equipment and technology was studied for dealing with such a problem in this paper. The composting equipment, which is composed of 4 bins with 1.56 m3 volume of each bin, was tested for 2 months at the municipal solid waste transfer station at Mengyong village, Panyu district in Guangzhou. The power of exhaust fan was 1.1 kW/h, the operation frequency was 10 times per day, 30 minutes per time, so the total operation time was 5 hours a day, the energy cost of the whole compost was 5.5kW. Through 12 days of high temperature fermentation followed by 24 days of bag fermentation, the temperature could be up to 60 °C for at least 5 days, the volume reduction rate of the rural refuse could reach up to 40% after 12 days, and there was no secondary pollution in the whole compost. The results showed that the composting equipment and technology was a feasible way to realize quantitative reduction, harmlessness and resources recovery of the rural refuse.

  7. Bio-NCs - the marriage of ultrasmall metal nanoclusters with biomolecules (United States)

    Goswami, Nirmal; Zheng, Kaiyuan; Xie, Jianping


    Ultrasmall metal nanoclusters (NCs) have attracted increasing attention due to their fascinating physicochemical properties. Today, functional metal NCs are finding growing acceptance in biomedical applications. To achieve a better performance in biomedical applications, metal NCs can be interfaced with biomolecules, such as proteins, peptides, and DNA, to form a new class of biomolecule-NC composites (or bio-NCs in short), which typically show synergistic or novel physicochemical and physiological properties. This feature article focuses on the recent studies emerging at the interface of metal NCs and biomolecules, where the interactions could impart unique physicochemical properties to the metal NCs, as well as mutually regulate biological functions of the bio-NCs. In this article, we first provide a broad overview of key concepts and developments in the novel biomolecule-directed synthesis of metal NCs. A special focus is placed on the key roles of biomolecules in metal NC synthesis. In the second part, we describe how the encapsulated metal NCs affect the structure and function of biomolecules. Followed by that, we discuss several unique synergistic effects observed in the bio-NCs, and illustrate them with examples highlighting their potential biomedical applications. Continued interdisciplinary efforts are required to build up in-depth knowledge about the interfacial chemistry and biology of bio-NCs, which could further pave their ways toward biomedical applications.

  8. Desalting by crystallization: detection of attomole biomolecules in picoliter buffers by mass spectrometry. (United States)

    Gong, Xiaoyun; Xiong, Xingchuang; Wang, Song; Li, Yanyan; Zhang, Sichun; Fang, Xiang; Zhang, Xinrong


    Sensitive detection of biomolecules in small-volume samples by mass spectrometry is, in many cases, challenging because of the use of buffers to maintain the biological activities of proteins and cells. Here, we report a highly effective desalting method for picoliter samples. It was based on the spontaneous separation of biomolecules from salts during crystallization of the salts. After desalting, the biomolecules were deposited in the tip of the quartz pipet because of the evaporation of the solvent. Subsequent detection of the separated biomolecules was achieved using solvent assisted electric field induced desorption/ionization (SAEFIDI) coupled with mass spectrometry. It allowed for direct desorption/ionization of the biomolecules in situ from the tip of the pipet. The organic component in the assistant solvent inhibited the desorption/ionization of salts, thus assured successful detection of biomolecules. Proteins and peptides down to 50 amol were successfully detected using our method even if there were 3 × 10(5) folds more amount of salts in the sample. The concentration and ion species of the salts had little influence on the detection results.

  9. Bio-NCs--the marriage of ultrasmall metal nanoclusters with biomolecules. (United States)

    Goswami, Nirmal; Zheng, Kaiyuan; Xie, Jianping


    Ultrasmall metal nanoclusters (NCs) have attracted increasing attention due to their fascinating physicochemical properties. Today, functional metal NCs are finding growing acceptance in biomedical applications. To achieve a better performance in biomedical applications, metal NCs can be interfaced with biomolecules, such as proteins, peptides, and DNA, to form a new class of biomolecule-NC composites (or bio-NCs in short), which typically show synergistic or novel physicochemical and physiological properties. This feature article focuses on the recent studies emerging at the interface of metal NCs and biomolecules, where the interactions could impart unique physicochemical properties to the metal NCs, as well as mutually regulate biological functions of the bio-NCs. In this article, we first provide a broad overview of key concepts and developments in the novel biomolecule-directed synthesis of metal NCs. A special focus is placed on the key roles of biomolecules in metal NC synthesis. In the second part, we describe how the encapsulated metal NCs affect the structure and function of biomolecules. Followed by that, we discuss several unique synergistic effects observed in the bio-NCs, and illustrate them with examples highlighting their potential biomedical applications. Continued interdisciplinary efforts are required to build up in-depth knowledge about the interfacial chemistry and biology of bio-NCs, which could further pave their ways toward biomedical applications.

  10. Cell and biomolecule delivery for tissue repair and regeneration in the central nervous system. (United States)

    Elliott Donaghue, Irja; Tam, Roger; Sefton, Michael V; Shoichet, Molly S


    Tissue engineering frequently involves cells and scaffolds to replace damaged or diseased tissue. It originated, in part, as a means of effecting the delivery of biomolecules such as insulin or neurotrophic factors, given that cells are constitutive producers of such therapeutic agents. Thus cell delivery is intrinsic to tissue engineering. Controlled release of biomolecules is also an important tool for enabling cell delivery since the biomolecules can enable cell engraftment, modulate inflammatory response or otherwise benefit the behavior of the delivered cells. We describe advances in cell and biomolecule delivery for tissue regeneration, with emphasis on the central nervous system (CNS). In the first section, the focus is on encapsulated cell therapy. In the second section, the focus is on biomolecule delivery in polymeric nano/microspheres and hydrogels for the nerve regeneration and endogenous cell stimulation. In the third section, the focus is on combination strategies of neural stem/progenitor cell or mesenchymal stem cell and biomolecule delivery for tissue regeneration and repair. In each section, the challenges and potential solutions associated with delivery to the CNS are highlighted.

  11. Implementation of an ex situ stabilization technique at the Sand Springs superfund site to solidify and stabilize acid tar sludges involving a quick-lime based stabilization process and innovative equipment design

    Energy Technology Data Exchange (ETDEWEB)

    McManus, R.W. [SOUND Environmental Services, Inc., Dallas, TX (United States); Grajczak, P. [ARCO, Los Angeles, CA (United States); Wilcoxson, J.C. [ARCO, Plano, TX (United States); Webster, S.D. [Environmental Protection Agency, Dallas, TX (United States)


    An old refinery site was safely remediated a year before schedule and for 25% less than final engineering estimates for the stabilization remedy thanks to energetic project management and innovative design involving ex situ stabilization/solidification of acid tar sludges. A quicklime based process, Dispersion by Chemical Reaction (DCR{trademark}), was employed to solidify and stabilize (SS) over 103,000 cubic meters (135,000 cubic yards) of petroleum waste, mostly acidic tarry sludge. The SS process was selected over competing methods because it afforded minimal volume increase, could readily achieve Record of Decision (ROD) specified physical and chemical treatment goals, could be implemented with treatment equipment that minimized emissions, and could be performed with low reagent usage and at low cost. To ensure treatment goals were achieved and an accelerated schedule met, a custom designed and fabricated transportable treatment unit (TTU) was employed to implement the process. The treated material was visually soil-like in character, it was left in stockpiles for periods of time, and it was placed and compacted in the on site landfill using standard earth-moving equipment.

  12. Design and Implementation of Basic Data Mining System of Secondary Equipment%二次设备基础数据挖掘系统的设计与实现

    Institute of Scientific and Technical Information of China (English)



    给出面向二次系统辅助决策的供电局二次设备基础数据挖掘系统的技术框架,并从惠州供电局二次设备数据管理的现状及需求出发,设计数据挖掘系统的主要功能模块,主要的数据分析功能包括基础统计及关联规则、聚类分析等数据挖掘方法。最后介绍系统实现。%Presents the technical framework of the secondary equipment data mining system of Power Supply Bureau, which is designed for the deci-sion support of the secondary system in power grid. The main system functional modules are designed based on the analysis of the current situation and requirement of secondary equipment data management in Huizhou Power Supply Bureau. Main data analysis methods in-clude basic statistics, association rules, clustering analysis and other data mining methods. Finally, the implementation of the system is in-troduced.

  13. A molecular dynamics study on the transport of a charged biomolecule in a polymeric adsorbent medium and its adsorption onto a charged ligand. (United States)

    Riccardi, E; Wang, J-C; Liapis, A I


    findings have significant practical implications to the design and construction of polymeric porous adsorbent media for effective bioseparations and to the synthesis and operation of processes employed in the separation of biomolecules. The modeling and analysis methods presented in this work could also be suitable for the study of biocatalysis where an enzyme is immobilized on the surface of the pores of a porous medium.

  14. Design of remainder particle auto-detection system for aerospace electronic equipment%航天电子设备多余物自动检测系统的研制

    Institute of Scientific and Technical Information of China (English)

    张坤; 戚乐; 陈金豹; 翟国富


    Remainder particle is a very important factor for the reliability of aerospace electronic equipment. Due to the existing detection system for remainder detection with low precision and couldn't drive the turntable automatically, a remainder particle auto-detection system for aerospace electronic equipment based on turntable driven by motor is designed. The design of the hardware and software of the system is mainly introduced. The hardware design includes mechanical structure, drive and control module, acceleration feedback circuit and data acquisition card. The software design includes the function of the system and the mainly algorithms. The experiments verify that the new remainder particle auto-detection system for aerospace electronic equipment designed in this paper can drive the turntable automatically,and the precision of remainder detection and material identification both satisfy the requirements.%多余物的存在是影响航天电子设备可靠性的重要因素.针对现有检测装置多余物检测精度低且不能实现转台自动控制的问题,研制了基于电机驱动转台的航天电子设备多余物自动检测系统.重点介绍了该系统的硬件设计和软件设计,硬件设计部分主要包括机械装置、驱动控制电路、加速度反馈电路和数据采集电路的具体实现,软件设计部分主要介绍了该系统的软件功能和核心算法.实验表明,该检测系统实现了转台的自动控制,并且多余物有无检测准确率和多余物材质识别准确率均达到设计指标.

  15. 精密仪器设备预约模块的设计和实现%Design and Implementation of an Appointment Module for Precision Instrument and Equipment

    Institute of Scientific and Technical Information of China (English)



    以Microsoft Visual Studio作为开发基础平台,C#为开发语言,IIS使用Windows自带程序,数据库使用MsSQL或者Access,采用B/S架构,Internet Explorer作为主要的网页浏览器对高校仪器设备管理系统中的预约模块进行设计,介绍了系统整体架构、预约模块各个功能分析和结构设计、预约流程和系统实现。%The paper mainly applies the modern computer technology to the design and implementation of the instrument and equipment system in the colleges and universities. It introduces the system architecture, the functional analysis, the structural design, the reservation procedure and the system implementation in each reservation module.

  16. Design and analysis of sodium carbonate recycling drying equipment for cyclohexanone production%环己酮生产中碳酸钠回收干燥装置的设计与分析

    Institute of Scientific and Technical Information of China (English)



    A new type of drying equipment was designed according to the characteristics of the recycling and utilization of waste sodium carbonate as the by-product of alkali waste burning in cyclohexanone process. The drying process, structure and working principle of the drying equipment were analyzed and calculated. The results showed that the automatic recycling drying equipment for sodium carbonate was consist of drying box, heating air system, dehumidifying system, scattering system and packaging system. The drying curves of sodium carbonate drying equipment were obtained through the drying experiment using a temperature-control electric oven. The drying capacity of this system reached 1 -2 t/h and the moisture content of sodium carbonate was below 2% after drying. The system saved the drying cost without environment pollution by using process gas waste as the heat re-souce. This equipment had the advantages of high drying capacity, high thermal efficiency, low energy consumption and good regulation performance, which successfully resolved the problem of alkali waste treatment in cyclohexanone industry.%针对环己酮生产过程中废碱碳酸钠回收利用的工艺特点,设计了一种新型干燥装置,对干燥器的干燥流程、干燥器的结构和工作原理进行了分析及计算.结果表明:循环自动干燥碳酸钠设备主要由干燥箱、供热风系统、抽湿系统、扩散系统和包装系统组成;通过温控由电烘箱进行碳酸钠干燥实验,得到了碳酸钠干燥设备的干燥曲线,该系统干燥能力达1 ~2 t/h,干燥后碳酸钠含水率小于2%;该系统利用工厂废烟气作热源,节省干燥成本,且无环境污染.该设备干燥能力大,热效率高,能耗低,调控性能好,很好的解决了环己酮工业中废碱处理的难题.

  17. Dynamics for heavy equipment airdrop and control design%重装空投动力学分析与控制方案研究

    Institute of Scientific and Technical Information of China (English)

    韩艳铧; 陆宇平


    A tow-body system of plane-cargo' s dynamics model is built based on the Lagrange analytical mechanics for a heavy equipment airdrop mission. A compensation scheme is proposed to weaken the cargo' s impact to airplane. Numerical simulation shows the dynamic coupling between airplane and cargoes, and the relevancy between the size of extraction parachute and simulation results is analyzed. Numerical simulation results also supports the effectiveness of control law proposed in this paper, that is, flying qualities and safety of airplane are improved significantly.%针对重装空投任务,基于拉格朗日分析力学,建立了货物在机舱内滑行阶段飞机-货物两体系统的动力学模型.给出了一种控制补偿方案,用以抵消或削弱货物运动对飞机的影响.通过数值仿真,观察货物运动和飞机运动之间的耦合关系,分析了牵引伞尺寸对飞机和货物运动参数的影响.仿真结果表明,所给出的控制补偿方案能够有效改善飞机飞行品质,提高飞行安全性.

  18. 智能脊柱理疗设备摆动机构设计与分析%Design and analysis of swingi ng mechanism of the intelligent spine physiotherapy equipment

    Institute of Scientific and Technical Information of China (English)

    张凯; 何非; 李东波


    In order to design the swinging mechanism that meets the functional requirements of the intelligent spine physiotherapy equipment , it compares the structural characteristics of several typical swinging mechanism , designs the mechanism for ensuring the realization of the function .Based on SolidWorks it establishes the whole 3D model,designs that the maximum swing speed is 100 times/min, the maximum load of the intelligent spine physiotherapy equipment is 100 kg.A dynamics simulation is performed with ADAMS to simulate the actual mo-tion of the motor , provides reasonable reference for motor selection .A dynamic analysis of the key shaft is per-formed with the finite element software ABAQUS for the optimization design of the shaft .The result shows that the swinging mechanism meets the functional requirements .%为设计满足智能脊柱理疗设备功能要求的摆动机构,对比了多种典型的摆动机构特点,设计了主体机构,同时为确保功能实现设计了辅助机构,并在SolidWorks 中构建了整体三维模型.设计最大摆动速度为100次/min,智能脊柱理疗设备最大承重为100kg. 通过ADAMS动力学仿真模拟其真实环境下的运动情况,对电机合理选型;利用ABAQUS对摆动机构关键零件支撑轴进行显式动态分析,优化设计支撑轴. 仿真与分析结果表明,摆动机构满足功能要求.

  19. Lightweight Design of Special Emergency Lifting Device of a Special Equipment%特种装备专用应急起重装置轻量化设计

    Institute of Scientific and Technical Information of China (English)

    郑艳琴; 向敢; 刘建华; 柯争华; 方利卡; 翁泽宇


    In according to the needs of use, maintenance and repair of a special equipment, and make clear the basic requirements of design of special emergency lifting device of a special equipment, the paper introduced the overall plan of design of this special emergency lifting device. Proceeded to finite element analysis on this lifting device with software ANSYS and checked the intensity of overall structure. Introduced a concept of lightweight design and carried on structure optimization on overall structure of this special emergency lifting device and focused on weight reduction on side plate of girder. The finite element analysis results show that the new design of overall structure of the emergency lifting device can meet the required intensity and at the same time the whole weight can reduce from 220 kg to 181 kg compare with the original project. [ Ch,6 fig. 11 ref. ]%根据某特种装备使用、维护和维修的需要,明确该设备专用应急起重装置的设计基本要求,确定总体方案设计.利用有限元软件ANSYS对该起重装置整体结构进行有限元分析,验算整体结构的强度.引入轻量化设计理念,对该专用应急起重装置整体结构进行结构优化,大粱侧板进行了重点的减重处理.有限元分析结果表明,新设计的应急起重装置总体结构在满足强度要求的同时,整体重量从原结构方案的220 kg减轻到181 kg.

  20. Data integrity design on accounting treatment system for music equipment management%音乐器材管理账务处理系统数据完整性设计

    Institute of Scientific and Technical Information of China (English)



    This paper conducted data integrity design based on the database design on accounting treatment system for music equipment management.Entity integrity was achieved by the primary key,and it designed primary key of three tables and described the syntax when created primary key;referential integrity was achieved by foreign key,and it designed foreign key which has two one-to-many relationships and explained the syntax when created foreign key;user-defined integrity was achieved by designing the corresponding constraint for relevant fields.%本文以音乐器材管理账务处理系统数据库设计为基础,进行数据完整性设计。实体完整性通过主关键字实现,设计了三个表的主关键字,并说明了创建主关键字的语法;参照完整性通过外关键字实现,设计了两个一对多关系的外关键字,并说明了创建外关键字的语法;用户自定义完整性通过为有关字段设计相应的约束实现。

  1. 一种压差式油水界面仪的设计与应用%Design and Application of Pressure Differential Oil-Water Interface Equipment

    Institute of Scientific and Technical Information of China (English)

    王永亮; 邱宏安; 尚娟


    This paper develops a new oil-water interface equipment based on the microchip MSC1210 and pressure differential transmitter. The pressure transmitter with high-precision was used for pressure measurement, and, it applies high precision A/D converter to data collection. To measure and display the height of the oil-water separate interface, relevant software is used to modify parameters. The whole process can indicate and control the discharge time. The application shows that this system runs stably and accurately under industry field environment. The system is practical and worthy of using abroad.%针对目前国内某些油田中油水分离的检测技术仍较为落后的现状,提出一种采用MSC1210单片机为核心的油水界面仪的设计;系统使用高精度的压力变送器进行压力侧量,通过MSC1210的高精度A/D转换器对数据进行采集,使用软件对现场参数进行修正,完成油田开采中对油水界面高度的侧量和显示,指示或控制排放废水或排放油品的时间;应用结果表明,该检测系统在工业现场环境下运行稳定,检测精度高,具有一定的实用和推广价值.

  2. 民用飞机设计驾驶舱操纵设备的评估%Evaluation on Flight Cockpit Control Equipments of Civil Aircraft Design

    Institute of Scientific and Technical Information of China (English)

    丰立东; 赵京洲; 田金强


    人机工效影响着飞行员操纵飞机的感受,因此在民用飞机设计过程中,对人机工效进行及早考虑是非常重要的。在民用飞机设计的各个阶段,都需要适时邀请具有一定资质的飞行员对驾驶舱的人机工效进行评估。对民用飞机设计中飞控驾驶舱飞行员评估方法进行介绍,对评估的要点进行总结分析,供相关工程设计人员参考。%Human factors affect the feeling of pilots operating airplanes. It is necessary to take human factors into account at the beginning of designing civil aircraft. During each phase of designing civil aircraft, it is necessary to invite qualified pilots to evaluate human factors in cockpit when needed. A method of pilot evaluation related with flight cockpit control system in designing civil airplane is introduced and some important issues are summarized and analyzed so as to refer to other relevant engineers and designers when necessary.

  3. New surface mining equipment for the 80s

    Energy Technology Data Exchange (ETDEWEB)

    Laswell, B.J.; Laswell, G.W.


    A few short decades, has seen progress from the pick, shovel, double-jack and muleteam to today's colossal haultrucks, shovels, drills and draglines. To predict future equipment size, design and configuration U.S. equipment and product companies were surveyed. A distillation of many opinions and viewpoints and the explicit predictions of two leading equipment manufacturers is given.

  4. An orientation sensitive approach in biomolecule interaction quantitative structure-activity relationship modeling and its application in ion-exchange chromatography. (United States)

    Kittelmann, Jörg; Lang, Katharina M H; Ottens, Marcel; Hubbuch, Jürgen


    Quantitative structure-activity relationship (QSAR) modeling for prediction of biomolecule parameters has become an established technique in chromatographic purification process design. Unfortunately available descriptor sets fail to describe the orientation of biomolecules and the effects of ionic strength in the mobile phase on the interaction with the stationary phase. The literature describes several special descriptors used for chromatographic retention modeling, all of these do not describe the screening of electrostatic potential by the mobile phase in use. In this work we introduce two new approaches of descriptor calculations, namely surface patches and plane projection, which capture an oriented binding to charged surfaces and steric hindrance of the interaction with chromatographic ligands with regard to electrostatic potential screening by mobile phase ions. We present the use of the developed descriptor sets for predictive modeling of Langmuir isotherms for proteins at different pH values between pH 5 and 10 and varying ionic strength in the range of 10-100mM. The resulting model has a high correlation of calculated descriptors and experimental results, with a coefficient of determination of 0.82 and a predictive coefficient of determination of 0.92 for unknown molecular structures and conditions. The agreement of calculated molecular interaction orientations with both, experimental results as well as molecular dynamic simulations from literature is shown. The developed descriptors provide the means for improved QSAR models of chromatographic processes, as they reflect the complex interactions of biomolecules with chromatographic phases.

  5. 5kt/a液体二氧化硫装置的工艺设计与设备选型%Process design and equipment selection of 5 kt/a liquid sulphur dioxide plant

    Institute of Scientific and Technical Information of China (English)



    介绍了5 kt/a液体二氧化硫装置工艺设计和设备选型情况。该装置采用柠檬酸钠法工艺,工艺参数选择合理、设备选型得当,目前已稳定运行1年多时间,各项工艺指标均达到或超过设计值。实际消耗硫磺0.45 t/t、电110kWh/t、柠檬酸10kg/t、纯碱18kg/t、0.3~0.4 MPa蒸汽0.4 t/t。探讨了设计生产中几个需要注意的问题,如输送管道材质选择、SO3吸收塔进口气温控制、柠檬酸钠溶液浓度控制、吸收尾气处理等。%Process design and equipment selection of a 5 kt/a liquid sulphur dioxide plant are described,which uses sodium citrate process with reasonable process parameters and equipment and materials.The plant has operated for more than one year and every operation index reached or was superior to design value.Actual operation showed that the consumption of sulphur was 0.45 t/t,power 110 kWh/t,citric acid 10 kg/t,soda ash 18 kg/t and 0.3-0.4 MPa steam 0.4 t/t.Several issues that need attention in design and production are discussed,such as selection of pipeline material,control of inlet gas temperature of SO,absorption tower and sodium citrate concentration, and tail gas treatment.

  6. Development of Virtual Equipment for a Hydraulic Mechanics Experiment

    Institute of Scientific and Technical Information of China (English)

    CHAN Ying-Chieh; CHEN Ya-Hsin; KANG Shih-Chung; LEE Tim-Hau


    Laboratory class is very important in the education of hydraulic mechanics. However, it usually requires a high expenditure on equipment purchase and maintenance. Virtual equipment that renders the equipment in a virtual world and provides an interactive interface for users can be an ideal solution to reduce costs. In this research, we focus on developing virtual equipment for a Venturi tube experiment. We em-ployed multiple paper-prototypes to gather user feedback on the preliminary design. Following the final pro-totype, we then implemented the virtual equipment on computers. Interactive functions have been built into the virtual equipment. Users are able to manipulate the equipment and obtain real-time feedback as if they were using actual equipment. The results show that the development of the virtual equipment is technically feasible.

  7. Electronic equipment packaging technology

    CERN Document Server

    Ginsberg, Gerald L


    The last twenty years have seen major advances in the electronics industry. Perhaps the most significant aspect of these advances has been the significant role that electronic equipment plays in almost all product markets. Even though electronic equipment is used in a broad base of applications, many future applications have yet to be conceived. This versatility of electron­ ics has been brought about primarily by the significant advances that have been made in integrated circuit technology. The electronic product user is rarely aware of the integrated circuits within the equipment. However, the user is often very aware of the size, weight, mod­ ularity, maintainability, aesthetics, and human interface features of the product. In fact, these are aspects of the products that often are instrumental in deter­ mining its success or failure in the marketplace. Optimizing these and other product features is the primary role of Electronic Equipment Packaging Technology. As the electronics industry continues to pr...

  8. Design and Implementation of Expert System for Measurement and Control Equipment Fault Diagnosis%测控装备故障诊断专家系统的设计与实现

    Institute of Scientific and Technical Information of China (English)



    通过分析测控装备故障诊断专家系统的目的和任务,将模糊神经网络理论与专家系统相结合用于测控装备故障诊断,建立了故障诊断专家系统的模型。探讨了故障诊断专家系统中知识库的构建及维护、不精确推理等问题。用面向对象分析方法分析故障诊断专家系统,并用软件加以实现。%Through the analysis of the purposes and tasks on fault diagnosis expert system of measurement and control system equipment, in this paper, the theory and method of fuzzy neural network were realized with the expert system that was used in fault diagnosis of test equipment and the fault diagnosis expert system model was established. Also, the problems of the knowledge forming, knowledge obtaining and inaccurate inference in fault diagnosis expert system were discussed. The analysis method gearing to the subject was used to analyze the design of the fault diagnosis expert system realized with the software

  9. Design and Realization of the Inspection & Pick—up Equipment for Wafer Microchip%晶圆微芯片检查提取设备的设计与实现

    Institute of Scientific and Technical Information of China (English)

    郭俭; 林海涛; 毕秋吉


    介绍了晶圆微芯片检查提取设备的工艺流程和技术要求,设计了各部分结构,特别对芯片提取翻转放置机构进行了详细说明.介绍了富士PLC、触摸屏、伺服电机和E-SX总线在该设备中的应用,该系统通过E-SX总线将人机界面、控制器和执行器件连接,配线简单、维护方便、高速可靠.%This article describes the process and technical requirements of the inspection & pick—up equipment for wafer microchip and the design of each part of the structure, especially giving a detailed description on the chip pick-up and flipping mechanism. It also gives an introduction on application of Fuji PLC, touch screen, servo motor and E—SX bus in the equipment. The system connects human-machine interface, controls and actuator via E-SX bus, wiring simple featuring simple wiring, easy maintenance, high speed and reliability.

  10. Design and Manufacture of the Traceable Solar Photovoltaic Power Generation Teaching Equipment%可追踪太阳能光伏发电教学装置的设计与制作

    Institute of Scientific and Technical Information of China (English)

    张春卿; 刘世泉; 李琪


    阐述了可追踪太阳能光伏发电教学装置的设计思路与制作过程.该教学装置能够体现一个完整的机电一体化系统,同时具有开环控制系统和闭环控制系统.其创新点是制作了自动追踪系统,可实现太阳能电池板对光源的自动追踪.它可以用于教学中,作为教学载体完成相关课程的教学项目开发,也可以用于专业二课堂的学习,提高学生对专业知识的学习兴趣.%This paper elaborates the design ideas and the making process of the traceable solar photovoltaic power generation teaching equipment. This teaching equipment can reflect a complete mechatronics system. It has open loop control system and the closed loop control system. Its innovation is to make the automatic tracking system, so the solar panels can track the light source automatically. It can be used as a carrier to develop a teaching program and also can be used for professional second classroom learning, improve students' interest in learning professional knowledge.

  11. Design of the Ancillary Equipment for Spent Fuel Transport of PWR Nuclear Power Plant%压水堆核电站乏燃料运输辅助设备设计

    Institute of Scientific and Technical Information of China (English)



    In order to load and unload spent fuel assemblies safely in NPP, the ancillary equipment for spent fuel transport used to perform fluid transfer activities such as water feeding, air charging, and cask drying and heat rejection. This paper introduces the requirements, the scheme and the principle of the ancillary equipment, which adopts centralized-control and modular design, and have the performances such as higher work efficiency, higher safety, less radioactive substance accumulation and friendly man-machine conversation.%乏燃料运输辅助设备是用于对乏燃料运输容器进行充水排气、充气排水、充气风干的设备,并可对已装载乏燃料组件的容器进行充水冷却,实现乏燃料组件的安全装卸.本文介绍乏燃料运输辅助设备的功能要求、设计方案和工作原理.设备采用模块化设计和集中控制,具有工作效率高、结构简单、安全性高、不易积聚放射性物质和人-机交互友好的特点.

  12. Quarry Equipment Yearbook 2003

    Energy Technology Data Exchange (ETDEWEB)



    This issue of the journal is devoted to an annual review of developments in the equipment used for minerals extraction and recycling. For each of 12 types of equipment, an editorial review is presented of developments and a tabular presentation of suppliers' ranges and contact information, including e-mail addresses and websites. The sections are: asphalt plants, conveyors, crushers and screens, drill rigs, dump trucks, excavators, hammers, pumps, tyres, weighbridges, wheel loaders and wheel washers.

  13. Incidents of chemical reactions in cell equipment

    Energy Technology Data Exchange (ETDEWEB)

    Baldwin, N.M.; Barlow, C.R. [Uranium Enrichment Organization, Oak Ridge, TN (United States)


    Strongly exothermic reactions can occur between equipment structural components and process gases under certain accident conditions in the diffusion enrichment cascades. This paper describes the conditions required for initiation of these reactions, and describes the range of such reactions experienced over nearly 50 years of equipment operation in the US uranium enrichment program. Factors are cited which can promote or limit the destructive extent of these reactions, and process operations are described which are designed to control the reactions to minimize equipment damage, downtime, and the possibility of material releases.

  14. Design and Application of Solar Combination Drying Equipment for Chinese Wolfberry%枸杞太阳能组合干燥装置的设计与应用

    Institute of Scientific and Technical Information of China (English)

    马林强; 李明滨; 慕松; 王燕昌


    The solar combination drying equipment (the solar energy storage and utilization equipment with the combination of active and passive pattern was designed based on the energy-saving products and technology of heat pump,efficient heat collector,heat exchanger and thermal storage to shorten drying time of Chinese wolfberry and reduce drying cost and improve wolfberry quality.The results showed that the daily average momentary heat collecting efficiency of the new heat collector reaches 65.6%,17%higher than the traditional heat collector.The average drying period of the solar combination drying equipment to dry Chinese wolfberry is about 33h,about 55h less than the natural air drying pattern.The solar combination drying equipment with high energy conservation and emission reduction efficiency and high Chinese wolfberry quality can be used in drying of Chinese wolfberry.%为了缩短枸杞干燥时间、降低干燥成本并提高产品质量,结合热泵、高效集热器、换热器和蓄热等节能产品与技术,研究设计了枸杞太阳能组合干燥装置(集主动式和被动式相结合的太阳能储存与利用设备),并对该干燥装置进行了应用试验。结果表明:新型集热器日平均瞬时集热效率为65.6%,较传统集热器集热效率约高17%。枸杞干燥试验,枸杞太阳能组合干燥装置平均干燥周期约33 h,比自然晾晒干燥周期缩短约2/3,大量节省常规能源,且产品质量高,节能减排效果明显。

  15. Manufacture and maintenance of laboratory equipment

    Energy Technology Data Exchange (ETDEWEB)

    Hwang, Chang Kyu; Hwang, Bong Ha; Lee, Jong Wha; Kim, Jong Kyun; Yeum, Ki Eon; Park, Myung Ku; Hong, Soon Eon; Choi, Ho Young; An, Dae Kyu; An, Choon; Park, In Won; Kim, Kyung Sik; Lee, Won Jae; Yoon, Ki Byung; Kim, Heung Woo; Lee, In Bae; Yeom, Jeong Hyun; Lee, Chul Ku; Sin, Keon Ju; Oh, Wan Ho; Seo, Yong Chil; Yang, Song Yeul; Lee, Young Soon; Choi, Byung Kwon; Chang, Kyung Duk [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)


    This report on the manufacture and maintenance of laboratory equipment contains working contents, scope and contents of request working to the machine shop, also records the principal production design, manufacture contents and a relevant working drawing. The working content and scope of the machine shop is to support the successful and convenient performance of the R and D by manufacture and maintenance of all sorts of laboratory equipment. 11 tabs., 3 figs. (Author) .new.

  16. Solar Drying of Tropical Forage Equipment Design%太阳能干燥热带牧草设备设计研究

    Institute of Scientific and Technical Information of China (English)



    热带牧草的干燥品质关系着热带畜牧业的发展。由于海南岛太阳辐射强度大,因而设计了用于太阳能干燥热带牧草的平台,其主要由太阳能集热器、风机、干燥仓、风道和控制系统等组成。针对干燥平台设计优化了太阳能集热器,对其结构、吸热涂层材料、上下通道距离比值、串并联连接方式等对集热效率的影响进行实验研究,并设计一套自动跟踪太阳能集热器系统,最终确定优化的实验平台。太阳能干燥热带牧草成本低、环保、效率高,具有广阔的市场前景。%Tropical forage dry relation with tropical development of animal husbandry , Hainan island has strong solar ra-diation , design a solar drying tropical forage platform , the test platform consists of solar collectors , fans, dry warehouse , duct , control systems and other components .Design and optimization a drying platform for flat-plate solar collectors , do-ing experimental study on collector efficiency which impact by Its structure , absorbing coating material , the upper and lower channel distance ratio , series and parallel connections , etc.And to design a set of automatic tracking solar collector system ,ultimately determine the optimal test platform .Solar drying of tropical forages has low cost , green, high efficien-cy , and has broad market prospects .

  17. The formation of a multidisciplinary team for the design of a green building; La formation d`une equipe pluridisciplinaire pour la conception d`un batiment vert

    Energy Technology Data Exchange (ETDEWEB)

    Cote, E.


    The concept of a green building is based on the principle of designing an energy efficient building, to be constructed or renovated in such a way that the total energy system is sustainable. In practice this means that maintenance and operating costs can be kept to a minimum. This paper discussed the importance of gaining input from experts in energy systems, based on the conviction that cooperation and team work will make for a total energy system in which the various components will work in harmony with each other.

  18. Atlas风机防喘振辅助设计及应用%Design and Application of Surge-Proof for Atlas Fan Auxiliary Equipment

    Institute of Scientific and Technical Information of China (English)



    This paper mainly introduces the Atlas fan control circuit and analyzes the surging causes of fan. The design schemes optimizes surge-proof control and implementation for Ausmelt blowing technology on Atlas air blower.%本文主要介绍了Atlas风机的控制回路,并对风机喘振原因进行了分析.阐述了Ausmelt吹炼工艺中Atlas风机的防喘振优化设计方案与实施.

  19. Dual functionalized graphene oxide serves as a carrier for delivering oligohistidine- and biotin-tagged biomolecules into cells. (United States)

    Jana, Batakrishna; Mondal, Goutam; Biswas, Atanu; Chakraborty, Indrani; Saha, Abhijit; Kurkute, Prashant; Ghosh, Surajit


    A versatile method of dual chemical functionalization of graphene oxide (GO) with Tris-[nitrilotris(acetic acid)] (Tris-NTA) and biotin for cellular delivery of oligohistidine- and biotin-tagged biomolecules is reported. Orthogonally functionalized GO surfaces with Tris-NTA and biotin to obtain a dual-functionalized GO (DFGO) are prepared and characterized by various spectroscopic and microscopic techniques. Fluorescence microscopic images reveal that DFGO surfaces are capable of binding oligohistidine-tagged biomolecules/proteins and avidin/biotin-tagged biomolecules/proteins orthogonally. The DFGO nanoparticles are non-cytotoxic in nature and can deliver oligohistidine- and biotin-tagged biomolecules simultaneously into the cell.

  20. Constructing Optimal Coarse-Grained Sites of Huge Biomolecules by Fluctuation Maximization. (United States)

    Li, Min; Zhang, John Zenghui; Xia, Fei


    Coarse-grained (CG) models are valuable tools for the study of functions of large biomolecules on large length and time scales. The definition of CG representations for huge biomolecules is always a formidable challenge. In this work, we propose a new method called fluctuation maximization coarse-graining (FM-CG) to construct the CG sites of biomolecules. The defined residual in FM-CG converges to a maximal value as the number of CG sites increases, allowing an optimal CG model to be rigorously defined on the basis of the maximum. More importantly, we developed a robust algorithm called stepwise local iterative optimization (SLIO) to accelerate the process of coarse-graining large biomolecules. By means of the efficient SLIO algorithm, the computational cost of coarse-graining large biomolecules is reduced to within the time scale of seconds, which is far lower than that of conventional simulated annealing. The coarse-graining of two huge systems, chaperonin GroEL and lengsin, indicates that our new methods can coarse-grain huge biomolecular systems with up to 10,000 residues within the time scale of minutes. The further parametrization of CG sites derived from FM-CG allows us to construct the corresponding CG models for studies of the functions of huge biomolecular systems.

  1. Immobilization of biomolecules on cysteamine-modified polyaniline film for highly sensitive biosensing. (United States)

    Cai, Qi; Xu, Baojian; Ye, Lin; Di, Zengfeng; Zhang, Jishen; Jin, Qinghui; Zhao, Jianlong; Xue, Jian; Chen, Xianfeng


    We present a new cysteamine (CS)-modified polyaniline (PANI) film for highly efficient immobilization of biomolecules in biosensing technology. This electrochemical deposited PANI film treated with CS and glutaraldehyde could be employed as an excellent substrate for biomolecules immobilization. The parameters of PANI growth were optimized to obtain suitable surface morphology of films for biomolecules combination with the help of electron and atomic force microscopy. Cyclic voltammetry (CV) was utilized to illustrate the different electrochemical activities of each modified electrode. Due to the existence of sulfydryl group and amino group in CS, surface modification with CS was proven to reduce oxidized units on PANI film remarkably, as evidenced by both ATR-FTIR and Raman spectroscopy characterizations. Furthermore, bovine serum albumin (BSA) was used as the model protein to investigate the immobilization efficiency of biomolecules on the PANI film, comparative study using quartz crystal microbalance (QCM) showed that BSA immobilized on CS-modified PANI could be increased by at least 20% than that without CS-modified PANI in BSA solution with the concentration of 0.1-1mg/mL. The CS-modified PANI film would be significant for the immobilization and detection of biomolecules and especially promising in the application of immunosensor for ultrasensitive detection.

  2. Role of PAMAM-OH dendrimers against the fibrillation pathway of biomolecules. (United States)

    Sekar, Gajalakshmi; Florance, Ida; Sivakumar, A; Mukherjee, Amitava; Chandrasekaran, Natarajan


    The binding behavior of nanoparticle with proteins determines its biocompatibility. This study reports the interaction of ten different biomolecules (proteins-BSA, HSA, haemoglobin, gamma globulin, transferrin and enzymes-hog and bacillus amylase, lysozyme from chicken and human and laccases from Tramates versicolor) with a surface group hydroxylated Poly AMido AMide dendrimer (PAMAM) of generation 5. The study has utilized various spectroscopic methods like UV-vis spectroscopy, Fluorescence emission, Synchronous, 3-D spectroscopy and Circular Dichroism to detect the binding induced structural changes in biomolecules that occur upon interaction with mounting concentration of the dendrimers. Aggregation of proteins results in the formation of amyloid fibrils causing several human diseases. In this study, fibrillar samples of all ten biomolecules formed in the absence and the presence of dendrimers were investigated with Congo Red absorbance and ThT Assay to detect fibril formation, Trp Emission and 3-D scan to evaluate the effect of fibrillation on aromatic environment of biomolecules, and CD spectroscopy to measure the conformational changes in a quantitative manner. These assays have generated useful information on the role of dendrimers in amyloid fibril formation of biomolecules. The outcomes of the study remain valuable in evaluating the biological safety of PAMAM-OH dendrimers for their biomedical application in vivo.

  3. Fabrication of biomolecules self-assembled on Au nanodot array for bioelectronic device. (United States)

    Lee, Taek; Kumar, Ajay Yagati; Yoo, Si-Youl; Jung, Mi; Min, Junhong; Choi, Jeong-Woo


    In the present study, an nano-platform composed of Au nanodot arrays on which biomolecules could be self-assembled was developed and investigated for a stable bioelectronic device platform. Au nanodot pattern was fabricated using a nanoporous alumina template. Two different biomolecules, a cytochrome c and a single strand DNA (ssDNA), were immobilized on the Au nanodot arrays. Cytochorme c and single stranded DNA could be immobilized on the Au nanodot using the chemical linker 11-MUA and thiol-modification by covalent bonding, respectively. The atomic structure of the fabricated nano-platform device was characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The electrical conductivity of biomolecules immobilized on the Au nanodot arrays was confirmed by scanning tunneling spectroscopy (STS). To investigate the activity of biomolecule-immobilized Au-nano dot array, the cyclic voltammetry was carried out. This proposed nano-platform device, which is composed of biomolecules, can be used for the construction of a novel bioelectronic device.

  4. Conditions to minimize soft single biomolecule deformation when imaging with atomic force microscopy. (United States)

    Godon, Christian; Teulon, Jean-Marie; Odorico, Michael; Basset, Christian; Meillan, Matthieu; Vellutini, Luc; Chen, Shu-Wen W; Pellequer, Jean-Luc


    A recurrent interrogation when imaging soft biomolecules using atomic force microscopy (AFM) is the putative deformation of molecules leading to a bias in recording true topographical surfaces. Deformation of biomolecules comes from three sources: sample instability, adsorption to the imaging substrate, and crushing under tip pressure. To disentangle these causes, we measured the maximum height of a well-known biomolecule, the tobacco mosaic virus (TMV), under eight different experimental conditions positing that the maximum height value is a specific indicator of sample deformations. Six basic AFM experimental factors were tested: imaging in air (AIR) versus in liquid (LIQ), imaging with flat minerals (MICA) versus flat organic surfaces (self-assembled monolayers, SAM), and imaging forces with oscillating tapping mode (TAP) versus PeakForce tapping (PFT). The results show that the most critical parameter in accurately measuring the height of TMV in air is the substrate. In a liquid environment, regardless of the substrate, the most critical parameter is the imaging mode. Most importantly, the expected TMV height values were obtained with both imaging with the PeakForce tapping mode either in liquid or in air at the condition of using self-assembled monolayers as substrate. This study unambiguously explains previous poor results of imaging biomolecules on mica in air and suggests alternative methodologies for depositing soft biomolecules on well organized self-assembled monolayers.

  5. Different size biomolecules anchoring on porous silicon surface: fluorescence and reflectivity pores infiltration comparative studies

    Energy Technology Data Exchange (ETDEWEB)

    Giovannozzi, Andrea M.; Rossi, Andrea M. [National Institute for Metrological Research, Thermodynamic Division, Strada delle Cacce 91, 10135 Torino (Italy); Renacco, Chiara; Farano, Alessandro [Ribes Ricecrhe Srl, Via Lavoratori Vittime del Col du Mont 24, 11100 Aosta (Italy); Derosas, Manuela [Biodiversity Srl, Via Corfu 71, 25124 Brescia (Italy); Enrico, Emanuele [National Institute for Metrological Research, Electromagnetism Division, Strada delle Cacce 91, 10135 Torino (Italy)


    The performance of porous silicon optical based biosensors strongly depends on material nanomorphology, on biomolecules distribution inside the pores and on the ability to link sensing species to the pore walls. In this paper we studied the immobilization of biomolecules with different size, such as antibody anti aflatoxin (anti Aflatox Ab, {proportional_to}150 KDa), malate dehydrogenase (MDH, {proportional_to}36KDa) and metallothionein (MT, {proportional_to}6KDa) at different concentrations on mesoporous silicon samples ({proportional_to}15 nm pores diameter). Fluorescence measurements using FITC- labeled biomolecules and refractive index analysis based on reflectivity spectra have been employed together to detect the amount of proteins bound to the surface and to evaluate their diffusion inside the pores. Here we suggest that these two techniques should be used together to have a better understanding of what happens at the porous silicon surface. In fact, when pores dimensions are not perfectly tuned to the protein size a higher fluorescence signal doesn't often correspond to a higher biomolecules distribution inside the pores. When a too much higher concentration of biomolecule is anchored on the surface, steric crowd effects and repulsive interactions probably take over and hinder pores infiltration, inducing a small or absent shift in the fringe pattern even if a higher fluorescence signal is registered. (copyright 2011 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  6. 滤波装置中晶闸管移相触发电路的设计%Design of SCR Phasing Trigger Circuit in filter Equipment

    Institute of Scientific and Technical Information of China (English)

    魏翔宇; 师五喜


    针对滤波补偿装置中要求电容器投切速度快,无冲击电流、电压的问题,设计了一种基于晶闸管移相触发的电路。该电路实现了快速无冲击投切,设计简单,能很好地解决过零触发电路经常遇到的过零检测混乱而导致的误触发,以及触发需等待电容器放电,有一定延迟的问题。通过现场实验测试分析说明,此移相触发电路的良好实用性。%For the problems in filter compensation device of capacitor switching speed, no impact current and overvoltage, the paper designs a circuit based on thyristor phasing trigger. The circuit have achieved fast switched and simply designed. It can solve chaos trigger,when the zero trigger circuit often encounter errors due to the zero crossing detection,and those delays when trigger the capacitor discharge .Through the field experiment test and analysis, illustrates that good practicability of phase shift trigger circuit.

  7. 某型装备随动系统检测诊断设备设计与实现%Design and Implementation of Fault Diagnosis Aparatus for a Follow-up System of Certain Equipment

    Institute of Scientific and Technical Information of China (English)

    石志勇; 王毅; 王怀光; 全振中


    In order to establish a test macro for a follow-up system of cetain equipment, this article designs one portable aparatus for fault diagnosis. Firstly, this article puts forward a design fundamental. Next, under this fundamental this article progresses some design. With opto-electronic coupler we implemented signal isolating, and applying resistance identifying circuit we got the poka yoke design, using these technic we could improve the security; the aparatus simulated the controled signal, which advanced the automatization; in the software design we adopted the modularization design, and joined expert system principle. It is proved that this system, featuring simple to operate, friendly interface, can greatly improve the efficiency of fault diagnose.%为实现对某型装备随动系统进行快速、准确地故障诊断,设计了一种便携式的检测诊断设备.首先提出设计原则,然后遵循设计原则进行了一系列设计.利用光电耦合器实现信号隔离,利用电阻识别电路实现防差错设计,增强设备使用的安全性;检测设备模拟计算机控制系统的控制信号提高了自动化程度;软件设计采用模块化设计,并融人专家系统原理,增强设备的智能性.经实验,该设备使用效果良好,系统操作简单,测试精度高,能大幅提高检测效率,有效地完成了测试任务.

  8. 辅助工装的设计与万向轴法兰叉头端面齿的加工方法%The Design of Assistant Equipment and Manufacturing Method of the Cardan Shaft Flange Fork Gearing

    Institute of Scientific and Technical Information of China (English)

    刘体龙; 李秋生; 李蒙


    According to the characters of the cardan shaft flange fork gearing, an assistant equipment was designed which could be used to manufacture the flange fork gearing in a six-gear method without changing the structure of X6142A universal lifting platform milling machine ( or the X5042A vertical lifting milling machine) .%针对万向轴法兰叉头端面齿形结构的特点,设计了一种辅助工装,利用该工装,在不改变X6142A万能升降台(或X5042A立式升降台)铣床本身结构的基础上即可对法兰叉头端面齿同时进行六齿加工。

  9. 基于EON的弹射救生设备虚拟维修训练系统设计与实现%Design and Realization of Virtual Maintenance Training System for Ejection Rescue Equipment Based on EON

    Institute of Scientific and Technical Information of China (English)

    李林; 李海军; 梁宇


    In accordance with the engineering practice, function requirement of Virtual Maintenance Training System for ejection rescue equipment was analyzed. The requirements and rules about the design of this System was identified. The software and hardware configuration scheme was fixed. Finally, a suit of simulation system based on EON was developed, which has been testified effective in meeting the needs of training for unit.%从工程实际出发,对弹射救生设备虚拟维修训练系统的功能需求进行了分析,明确了系统的设计要求和设计准则,确定了系统的软硬件配置方案,开发了一套基于EON的仿真系统.实际应用表明,这一系统可以满足部队训练需求.

  10. Large dynamic range digital nanodot gradients of biomolecules made by low-cost nanocontact printing for cell haptotaxis. (United States)

    Ricoult, Sébastien G; Pla-Roca, Mateu; Safavieh, Roozbeh; Lopez-Ayon, G Monserratt; Grütter, Peter; Kennedy, Timothy E; Juncker, David


    A novel method is introduced for ultrahigh throughput and ultralow cost patterning of biomolecules with nanometer resolution and novel 2D digital nanodot gradients (DNGs) with mathematically defined slopes are created. The technique is based on lift-off nanocontact printing while using high-resolution photopolymer stamps that are rapidly produced at a low cost through double replication from Si originals. Printed patterns with 100 nm features are shown. DNGs with varying spacing between the dots and a record dynamic range of 4400 are produced; 64 unique DNGs, each with hundreds of thousands of dots, are inked and printed in 5.5 min. The adhesive response and haptotaxis of C2C12 myoblast cells on DNGs demonstrated their biofunctionality. The great flexibility in pattern design, the massive parallel ability, the ultra low cost, and the extreme ease of polymer lift-off nanocontact printing will facilitate its use for various biological and medical applications.

  11. The Design and Implementation of a Communication Interface Circuit in anAirborne Equipment%一种机载设备的通讯接口电路的设计与实现

    Institute of Scientific and Technical Information of China (English)

    王桢; 熊育信; 童鑫


      The design and implementation of a communication interface circuit in airborne equipment is introduced, which is an important part of the interface box in airborne equipment. The circuit receives the command information output by 422 interface of the integrated processor, such as target bearing, target distance, target designation, and target effective, and sends it to the fire control system after format conversion and electrical match. Moreover, the circuit will make D/C conversion etc. for cable height digital signal, send it to aircraft's control system and hover device.%  文章介绍了一种机载设备的通讯接口电路的设计与实现,该接口电路是机载设备接线盒的重要组成部分,其设计目标主要是接收前端的综合处理机通过422接口输出的目标方位、目标距离、目标编号、目标有效等命令信息,对其进行格式转换和电气匹配后,再送到发控系统。此外,该通讯接口电路还对接收到的干缆高度数字信号进行数模转换等相关处理,并发送到飞机控制系统和悬停器。

  12. Design of the Remote Monitoring and Fault Diagnosis System for Power Electronic Equipment%电力电子设备远程监控与故障诊断系统设计

    Institute of Scientific and Technical Information of China (English)

    马皓; 韩思亮


    针对电力电子设备中监控与故障诊断实时性要求高、数据传输量大、诊断原因复杂的特点,提出了一种具有强兼容性的基于工控机(IPC)和PLC现场控制分布式远程监控与故障诊断系统的新思路.结合电力电子技术、故障诊断技术以及网络技术,设计开发了一种三层C/S结构与Internet结合的电力电子设备远程监控与故障诊断网络系统,着重介绍了具有强兼容性的系统结构、现场监控兼容性设计、数据采集子系统和实时监控子系统等各部分子系统的设计和通信协议设计开发.%To meet the requirement of highly real-time, tremendous data transferring and complicated diagnostic causes of remote monitoring and fault diagnosis with power electronic equipment, a novel idea of distributed system with industrial PC (IPC) and PLC controlling field terminal is proposed in this paper. Based on the techniques of power electronics, fault diagnosis and network, a remote monitoring and fault diagnosis system with combination of a three-layer C/S structure and Internet is presented, which achieves with high compatibility between different equipment. Furthermore, the paper focuses on the discussion about the structure of compatible systems, the compatibility design of remote monitoring and the realization of field control equipment, such as the data collection sub-system, the real-time monitoring sub-system, the fault diagnosis sub-system and the data disposal and management sub-system, and the design of the data transferring sub-system and the communication protocol format.This work is supported by National Natural Science Foundation of China (No. 50207008).

  13. 发酵设备课程设计教学中抄袭现象的研究%Course Design of Plagiarism Phenomenon in Fermentation Engineering and Equipment

    Institute of Scientific and Technical Information of China (English)

    张超; 陈文兵; 武道吉; 侯书国; 郑晓吉


    As an important link of practice teaching, the fermentation engineering and equipment course design can play the connecting role between the preceding and the following in the practice teaching, and lay a good foundation for the students to work in the factory after graduation. There exist all different plagiarism phenomenon during the course design. The article shall exchange and discuss the teaching links, such as the status, opinion, contents and way of the course design.%发酵设备课程设计是重要的实践教学环节,在实践教学中起到衔接作用,可为学生进入工作岗位打下良好基础。但在课程设计教学过程中,学生之间存在不同程度的抄袭现象,对课程设计中抄袭现象产生的原因、解决方法和效果等各环节进行交流与探讨。

  14. Affinity-Mediated Homogeneous Electrochemical Aptasensor on a Graphene Platform for Ultrasensitive Biomolecule Detection via Exonuclease-Assisted Target-Analog Recycling Amplification. (United States)

    Ge, Lei; Wang, Wenxiao; Sun, Ximei; Hou, Ting; Li, Feng


    As is well-known, graphene shows a remarkable difference in affinity toward nonstructured single-stranded (ss) DNA and double-stranded (ds) DNA. This property makes it popular to prepare DNA-based optical sensors. In this work, taking this unique property of graphene in combination with the sensitive electrochemical transducer, we report a novel affinity-mediated homogeneous electrochemical aptasensor using graphene modified glassy carbon electrode (GCE) as the sensing platform. In this approach, the specific aptamer-target recognition is converted into an ultrasensitive electrochemical signal output with the aid of a novel T7 exonuclease (T7Exo)-assisted target-analog recycling amplification strategy, in which the ingeniously designed methylene blue (MB)-labeled hairpin DNA reporters are digested in the presence of target and, then, converted to numerous MB-labeled long ssDNAs. The distinct difference in differential pulse voltammetry response between the designed hairpin reporters and the generated long ssDNAs on the graphene/GCE allows ultrasensitive detection of target biomolecules. Herein, the design and working principle of this homogeneous electrochemical aptasensor were elucidated, and the working conditions were optimized. The gel electrophoresis results further demonstrate that the designed T7Exo-assisted target-analog recycling amplification strategy can work well. This electrochemical aptasensor realizes the detection of biomolecule in a homogeneous solution without immobilization of any bioprobe on electrode surface. Moreover, this versatile homogeneous electrochemical sensing system was used for the determination of biomolecules in real serum samples with satisfying results.

  15. Finding Semirigid Domains in Biomolecules by Clustering Pair-Distance Variations

    Directory of Open Access Journals (Sweden)

    Michael Kenn


    Full Text Available Dynamic variations in the distances between pairs of atoms are used for clustering subdomains of biomolecules. We draw on a well-known target function for clustering and first show mathematically that the assignment of atoms to clusters has to be crisp, not fuzzy, as hitherto assumed. This reduces the computational load of clustering drastically, and we demonstrate results for several biomolecules relevant in immunoinformatics. Results are evaluated regarding the number of clusters, cluster size, cluster stability, and the evolution of clusters over time. Crisp clustering lends itself as an efficient tool to locate semirigid domains in the simulation of biomolecules. Such domains seem crucial for an optimum performance of subsequent statistical analyses, aiming at detecting minute motional patterns related to antigen recognition and signal transduction.

  16. Radical Reactions in the Gas Phase: Recent Development and Application in Biomolecules

    Directory of Open Access Journals (Sweden)

    Yang Gao


    Full Text Available This review summarizes recent literature describing the use of gas phase radical reactions for structural characterization of complex biomolecules other than peptides. Specifically, chemical derivatization, in-source chemical reaction, and gas phase ion/ion reactions have been demonstrated as effective ways to generate radical precursor ions that yield structural informative fragments complementary to those from conventional collision-induced dissociation (CID. Radical driven dissociation has been applied to a variety of biomolecules including peptides, nucleic acids, carbohydrates, and phospholipids. The majority of the molecules discussed in this review see limited fragmentation from conventional CID, and the gas phase radical reactions open up completely new dissociation channels for these molecules and therefore yield high fidelity confirmation of the structures of the target molecules. Due to the extensively studied peptide fragmentation, this review focuses only on nonpeptide biomolecules such as nucleic acids, carbohydrates, and phospholipids.

  17. Benzophenone-based photochemical micropatterning of biomolecules to create model substrates and instructive biomaterials. (United States)

    Turgeon, Aurora J; Harley, Brendan A; Bailey, Ryan C


    The extracellular matrix (ECM) is a dynamic and heterogeneous environment that controls many aspects of cell behavior. Not surprisingly, many different approaches have focused on creating model substrates that recapitulate the biomolecular, topographical, and mechanical properties of the ECM for in vitro studies of cell behavior. This chapter details a general, versatile method for the spatially controlled deposition of multiple biomolecules onto both planar and topographically complex support structures with micrometer resolution. This approach is based upon the well-understood photochemical UV crosslinking of benzophenone (BP) to solution-phase biomolecules. This is a molecularly general strategy that can be utilized to immobilize biomolecules onto any surface prefunctionalized with BP. Examples described herein include modification of planar and corrugated glass substrates as well as collagen-glycosaminoglycan biomaterials configured either as highly porous scaffolds or nonporous membranes with a variety of biomolecular targets, including proteins, glycoproteins, and carbohydrates.

  18. SERS imaging of cell-surface biomolecules metabolically labeled with bioorthogonal Raman reporters. (United States)

    Xiao, Ming; Lin, Liang; Li, Zefan; Liu, Jie; Hong, Senlian; Li, Yaya; Zheng, Meiling; Duan, Xuanming; Chen, Xing


    Live imaging of biomolecules with high specificity and sensitivity as well as minimal perturbation is essential for studying cellular processes. Here, we report the development of a bioorthogonal surface-enhanced Raman scattering (SERS) imaging approach that exploits small Raman reporters for visualizing cell-surface biomolecules. The cells were cultured and imaged by SERS microscopy on arrays of Raman-enhancing nanoparticles coated on silicon wafers or glass slides. The Raman reporters including azides, alkynes, and carbondeuterium bonds are small in size and spectroscopically bioorthogonal (background-free). We demonstrated that various cell-surface biomolecules including proteins, glycans, and lipids were metabolically incorporated with the corresponding precursors bearing a Raman reporter and visualized by SERS microscopy. The coupling of SERS microscopy with bioorthogonal Raman reporters expands the capabilities of live-cell microscopy beyond the modalities of fluorescence and label-free imaging.

  19. Finding semirigid domains in biomolecules by clustering pair-distance variations. (United States)

    Kenn, Michael; Ribarics, Reiner; Ilieva, Nevena; Schreiner, Wolfgang


    Dynamic variations in the distances between pairs of atoms are used for clustering subdomains of biomolecules. We draw on a well-known target function for clustering and first show mathematically that the assignment of atoms to clusters has to be crisp, not fuzzy, as hitherto assumed. This reduces the computational load of clustering drastically, and we demonstrate results for several biomolecules relevant in immunoinformatics. Results are evaluated regarding the number of clusters, cluster size, cluster stability, and the evolution of clusters over time. Crisp clustering lends itself as an efficient tool to locate semirigid domains in the simulation of biomolecules. Such domains seem crucial for an optimum performance of subsequent statistical analyses, aiming at detecting minute motional patterns related to antigen recognition and signal transduction.

  20. Detection of biomolecules and bioconjugates by monitoring rotated grating-coupled surface plasmon resonance

    CERN Document Server

    Szalai, Aniko; Somogyi, Aniko; Szenes, Andras; Banhelyi, Balazs; Csapo, Edit; Dekany, Imre; Csendes, Tibor; Csete, Maria


    Plasmonic biosensing chips were prepared by fabricating wavelength-scaled dielectric-metal interfacial gratings on thin polycarbonate films covered bimetal layers via two-beam interference laser lithography. Lysozyme (LYZ) biomolecules and gold nanoparticle (AuNP-LYZ) bioconjugates with 1:5 mass ratio were seeded onto the biochip surfaces. Surface plasmon resonance spectroscopy was performed before and after biomolecule seeding in a modified Kretschmann-arrangement by varying the azimuthal and polar angles to optimize the conditions for rotated grating-coupling. The shift of secondary and primary resonance peaks originating from rotated grating-coupling phenomenon was monitored to detect the biomolecule and bioconjugate adherence. Numerical calculations were performed to reproduce the measured reflectance spectra and the resonance peak shifts caused by different biocoverings. Comparison of measurements and calculations proved that monitoring the narrower secondary peaks under optimal rotated-grating coupling ...

  1. Phthalocyanine-Biomolecule Conjugated Photosensitizers for Targeted Photodynamic Therapy and Imaging. (United States)

    Iqbal, Zafar; Chen, Jincan; Chen, Zhuo; Huang, Mingdong


    Photodynamic therapy (PDT) is now in clinical practice in many European and American countries as a minimally invasive therapeutic technique to treat oncologic malignancies and other nononcologic conditions. Phthalocyanines (Pcs) are gathering importance as effective photosensitizers in targeted PDT and imaging of tumors. The possibility of modification around the Pc macrocycle led the researchers to the synthesis of a diversity of photosensitizers with varied cell specificity, cellular internalization and localization, photodynamic cytotoxicity and excretion. Cellular targeting is the primary aspect of an ideal photosensitizer for targeting PDT. Therefore, Pcs have been structurally modified with a variety of biomolecules capable of recognizing the specific lesions. This review emphasizes the photocytotoxicity and the cellular uptakes of phthalocyanine photosensitizers conjugated with biomolecules including carbohydrates, nucleotides and protein constituents such as amino acids and peptides. In addition, the role of the Pc-biomolecule conjugates in imaging and antimicrobial chemotherapy has been discussed.

  2. 一种可移动组合式医疗废物处理装置的设计%The design of a removable combined medical wastes processing equipment

    Institute of Scientific and Technical Information of China (English)

    陈宏; 陈艳; 张剑鹏; 王轶丹; 王卫


    目的:设计一种可移动的组合式医疗废物处理装置,便于使用者方便高效地分类处理医疗废物。方法:根据人机工程学原理及医疗废物管理的要求进行设计,将医用锐器盒和垃圾桶有效组合为上、下桶体,L型托板的竖板内侧上端设有可调节的上桶体固定架。上桶体的剪刀可高效率按照要求处理医疗锐器;下桶体固定在L型托板上,托板的横板下表面设有多个万向轮,便于移动使用,下桶体的上盖可以方便的按要求开关活动盖。结果:实用新型的组合式医疗废物处理装置设计合理,内部结构可分区,装置外观整洁,可节约空间,且操作简便,能够体现人性化关怀。结论:组合式医疗废物处理装置适用于临床及医学院校实验(训)室等场所,对于医疗废物的分类处置具有实用意义及推广价值。%Objective:Design one kind of removable combined medical wastes processing equipment which can facilitate the users for classification treatment of medical waste conveniently and efficiently.Methods: The equipment design followed by the principle of ergonomics and medical waste management requirements. The medical sharps boxes and trash set together effectively, including the upper barrel and lower barrel. The adjustable barrel shelf was fixed on the inside of vertical plate of L-type plate. Scissors on the upper barrel handled medical sharp instrument efficiently. The lower barrel fixed on L-type plate, many universal wheels were set on the horizontal bottom surface of plate for mobile use. The cover of lower barrel can easily switch according to the requirements.Results: The combination of the utility model design and the reasonable structure of internal partitions make clean appearance of the equipment, saving space, easily to operate and embody the human nature concern.Conclusion: The medical wastes processing equipment was applied in clinical and medical

  3. 基于PLC的梳理机自调匀整系统的设计%Design of Auto- leveller System for Carding Equipment Based on PLC

    Institute of Scientific and Technical Information of China (English)

    周自强; 潘毅; 张伟强


    This paper presents a general technique scheme that used for carding machine of non - woven product line. An improved weighing mechanism is designed for acquiring the real time data of cotton sheet. The auto - leveller system based on the FX - PLC and GT100 touch screen as hardware. A fuzzy control algorithm is used to control the speed of roller by frequency - changing technology. Thus automatic controlling for longitudinal u-niformity of cotton sheet from carding machine is implemented.%设计了一种用于非织布生产线上梳理机自调匀整系统,采用了一种改进的棉网称重机构,利用三菱PLC和GT100触摸屏作为硬件平台,应用模糊控制方法对输棉帘的速度进行变频调速控制,从而实现了梳理机输出棉网纵向均匀度的自动控制.

  4. Design of Rolling Balance Experimental Equipment for LowSpeed Wind Tunnel%低速风洞旋转天平试验装置的设计

    Institute of Scientific and Technical Information of China (English)

    杨恩霞; 庞永刚; 刁彦飞


    By way of simulating trial of plane model whirligig in windtunnel by use of rolling balance and measuring the aerodynamic force on circumvoluting model,we can analyse the trial spinning characteristic.This is one of the efficient way. The paper introduced the working prinnciple and structural design of rolling balance for low speed wind tunnelindetail.%应用旋转天平使飞机模型在风洞里模拟飞机尾旋时的旋转运动,并测量出旋转模型上的气动力,从而对飞机的尾旋特性进行分析、预测,这是研究飞机尾旋特性的有效方法之一。较为详细地介绍了低速风洞旋转天平的工作原理及结构设计。

  5. Composite scaffold of poly(vinyl alcohol) and interfacial polyelectrolyte complexation fibers for controlled biomolecule delivery. (United States)

    Cutiongco, Marie Francene A; Choo, Royden K T; Shen, Nathaniel J X; Chua, Bryan M X; Sju, Ervi; Choo, Amanda W L; Le Visage, Catherine; Yim, Evelyn K F


    Controlled delivery of hydrophilic proteins is an important therapeutic strategy. However, widely used methods for protein delivery suffer from low incorporation efficiency and loss of bioactivity. The versatile interfacial polyelectrolyte complexation (IPC) fibers have the capacity for precise spatiotemporal release and protection of protein, growth factor, and cell bioactivity. Yet its weak mechanical properties limit its application and translation into a viable clinical solution. To overcome this limitation, IPC fibers can be incorporated into polymeric scaffolds such as the biocompatible poly(vinyl alcohol) hydrogel (PVA). Therefore, we explored the use of a composite scaffold of PVA and IPC fibers for controlled biomolecule release. We first observed that the permeability of biomolecules through PVA films were dependent on molecular weight. Next, IPC fibers were incorporated in between layers of PVA to produce PVA-IPC composite scaffolds with different IPC fiber orientation. The composite scaffold demonstrated excellent mechanical properties and efficient biomolecule incorporation. The rate of biomolecule release from PVA-IPC composite grafts exhibited dependence on molecular weight, with lysozyme showing near-linear release for 1 month. Angiogenic factors were also incorporated into the PVA-IPC grafts, as a potential biomedical application of the composite graft. While vascular endothelial growth factor only showed a maximum cumulative release of 3%, the smaller PEGylated-QK peptide showed maximum release of 33%. Notably, the released angiogenic biomolecules induced endothelial cell activity thus indicating retention of bioactivity. We also observed lack of significant macrophage response against PVA-IPC grafts in a rabbit model. Showing permeability, mechanical strength, precise temporal growth factor release, and bioinertness, PVA-IPC fibers composite scaffolds are excellent scaffolds for controlled biomolecule delivery in soft tissue engineering.

  6. Composite scaffold of poly(vinyl alcohol and interfacial polyelectrolyte complexation fibers for controlled biomolecule delivery

    Directory of Open Access Journals (Sweden)

    Marie Francene Arnobit Cutiongco


    Full Text Available Controlled delivery of hydrophilic proteins is an important therapeutic strategy. However, widely used methods for protein delivery suffer from low incorporation efficiency and loss of bioactivity. The versatile interfacial polyelectrolyte complexation (IPC fibers have the capacity for precise spatiotemporal release and protection of protein, growth factor and cell bioactivity. Yet its weak mechanical properties limit its application and translation into a viable clinical solution. To overcome this limitation, IPC fibers can be incorporated into polymeric scaffolds such as the biocompatible poly(vinyl alcohol hydrogel (PVA. Therefore, we explored the use of a composite scaffold of PVA and IPC fibers for controlled biomolecule release. We first observed that the permeability of biomolecules through PVA films were dependent on molecular weight, with lysozyme showing near-linear release for 1 month. Next, IPC fibers were incorporated in between layers of PVA to produce PVA-IPC composite scaffolds with different IPC fiber orientation. The composite scaffold demonstrated excellent mechanical properties and efficient biomolecule incorporation. The rate of biomolecule release from PVA-IPC composite grafts exhibited dependence on molecular weight. Angiogenic factors were also incorporated into the PVA-IPC grafts, as a potential biomedical application of the composite graft. While vascular endothelial growth factor only showed a maximum cumulative release of 3%, the smaller PEGylated-QK peptide showed maximum release of 33%. Notably, the released angiogenic biomolecules induced endothelial cell metabolic activity thus indicating retention of bioactivity. We also observed lack of significant macrophage response against PVA-IPC grafts in a rabbit model. Showing permeability, mechanical strength, precise temporal growth factor release and bioinertness, PVA-IPC fibers composite scaffolds are excellent scaffolds for controlled biomolecule delivery in soft

  7. Resistance welding equipment manufacturing capability for exports

    Energy Technology Data Exchange (ETDEWEB)

    Sastry, V.S.; Raju, Y.S.; Somani, A.K.; Setty, D.S.; Rameswara Raw, A.; Hermantha Rao, G.V.S.; Jayaraj, R.N. [Nuclear Fuel Complex, Dept. of Atomic Energy, Hyderbad (India)


    Indian Pressurised Heavy Water Reactor (PHWR) fuel bundle is fully welded and is unique in its design. Appendage welding, end closure welding, and end plate welding is carried out using resistance welding technique. Out of many joining processes available, resistance-welding process is reliable, environment friendly and best suitable for mass production applications. Nuclear Fuel Complex (NFC), an industrial unit is established in Hyderabad, under the aegis of the Dept of Atomic Energy to manufacture fuel for Pressurised Heavy Water Reactors. From inception, NFC has given importance for self-reliance and indigenization with respect to manufacturing process and equipment. Sintering furnaces, centreless grinders, appendage-welding machines, end-closure welding equipment and end-plate welding equipments, which were initially imported, are either indigenized or designed and manufactured in house. NFC has designed, manufactured a new appendage-welding machine for manufacturing 37 element fuel bundles. Recently NFC has bagged an order from IAEA through international bidding for design, manufacture, supply, erection and commissioning of end-closure welding equipment. The paper gives in detail the salient features of these welding equipment. (author)

  8. Design and testing of head-stem segregation equipment for garlic%大蒜果秧分离试验装置的设计与测试

    Institute of Scientific and Technical Information of China (English)

    于昭洋; 胡志超; 王海鸥; 彭宝良; 谢焕雄; 吴峰


    Garlic is one of the major cash crops in China. Production, acreage, and exports of garlic in China are the highest in the world. As a seasonal operation, garlic harvesting in China is still mainly manual work, having the problems of low efficiency, high labor intensity, and huge occupation of farming season, which has constrained the further development of the garlic industry. The current domestic garlic harvesters are basically simple segmented machinery. The techniques of garlic combine harvesting is still in its infancy. Picking is the core technology on garlic combine harvesting, but it has not been refined well. In order to enhance operational performance of picking, a new kind of garlic picking testbed was designed to carry out the studies on optimization design on structural and working parameters of a garlic-picking device. Lower damage and less stem are the two primary objectives in garlic harvesting. The garlic picking testbed consists of a clamping delivery device, a ranking/alignment device, a cutter, and an electric control system, which carry out garlic clamping, transport, ranking, alignment, and cutting at one time. The clamping delivery device of which core components are front track and middle track uses two tooth-shaped chains. Through the guidance of the two tracks, the trajectory and clamping style of the chains is changed, achieveing the transition from double chain clamping to chain-board-hairbrush clamping, The core components of the ranking/alignment devices are guidance rods, a ranking disk, finger, an alignment chain, and a hairbrush. In the test, the garlic was artificially fed. First, garlic plants are gripped by the clamping delivery device and transported backward. Under the interaction of ranking/alignment devices and clamping/delivery devices, garlic plants are ranked automatically and garlic bulbs are aligned automatically. After the garlic bulbs are aligned, garlic stems are cut by disc cutters to achieve garlic bulbs whose stems

  9. Equipment Operational Requirements

    Energy Technology Data Exchange (ETDEWEB)

    Greenwalt, B; Henderer, B; Hibbard, W; Mercer, M


    The Iraq Department of Border Enforcement is rich in personnel, but poor in equipment. An effective border control system must include detection, discrimination, decision, tracking and interdiction, capture, identification, and disposition. An equipment solution that addresses only a part of this will not succeed, likewise equipment by itself is not the answer without considering the personnel and how they would employ the equipment. The solution should take advantage of the existing in-place system and address all of the critical functions. The solutions are envisioned as being implemented in a phased manner, where Solution 1 is followed by Solution 2 and eventually by Solution 3. This allows adequate time for training and gaining operational experience for successively more complex equipment. Detailed descriptions of the components follow the solution descriptions. Solution 1 - This solution is based on changes to CONOPs, and does not have a technology component. It consists of observers at the forts and annexes, forward patrols along the swamp edge, in depth patrols approximately 10 kilometers inland from the swamp, and checkpoints on major roads. Solution 2 - This solution adds a ground sensor array to the Solution 1 system. Solution 3 - This solution is based around installing a radar/video camera system on each fort. It employs the CONOPS from Solution 1, but uses minimal ground sensors deployed only in areas with poor radar/video camera coverage (such as canals and streams shielded by vegetation), or by roads covered by radar but outside the range of the radar associated cameras. This document provides broad operational requirements for major equipment components along with sufficient operational details to allow the technical community to identify potential hardware candidates. Continuing analysis will develop quantities required and more detailed tactics, techniques, and procedures.

  10. Live-cell stimulated Raman scattering imaging of alkyne-tagged biomolecules. (United States)

    Hong, Senlian; Chen, Tao; Zhu, Yuntao; Li, Ang; Huang, Yanyi; Chen, Xing


    Alkynes can be metabolically incorporated into biomolecules including nucleic acids, proteins, lipids, and glycans. In addition to the clickable chemical reactivity, alkynes possess a unique Raman scattering within the Raman-silent region of a cell. Coupling this spectroscopic signature with Raman microscopy yields a new imaging modality beyond fluorescence and label-free microscopies. The bioorthogonal Raman imaging of various biomolecules tagged with an alkyne by a state-of-the-art Raman imaging technique, stimulated Raman scattering (SRS) microscopy, is reported. This imaging method affords non-invasiveness, high sensitivity, and molecular specificity and therefore should find broad applications in live-cell imaging.

  11. Metal Stable Isotope Tagging: Renaissance of Radioimmunoassay for Multiplex and Absolute Quantification of Biomolecules. (United States)

    Liu, Rui; Zhang, Shixi; Wei, Chao; Xing, Zhi; Zhang, Sichun; Zhang, Xinrong


    The unambiguous quantification of biomolecules is of great significance in fundamental biological research as well as practical clinical diagnosis. Due to the lack of a detectable moiety, the direct and highly sensitive quantification of biomolecules is often a "mission impossible". Consequently, tagging strategies to introduce detectable moieties for labeling target biomolecules were invented, which had a long and significant impact on studies of biomolecules in the past decades. For instance, immunoassays have been developed with radioisotope tagging by Yalow and Berson in the late 1950s. The later languishment of this technology can be almost exclusively ascribed to the use of radioactive isotopes, which led to the development of nonradioactive tagging strategy-based assays such as enzyme-linked immunosorbent assay, fluorescent immunoassay, and chemiluminescent and electrochemiluminescent immunoassay. Despite great success, these strategies suffered from drawbacks such as limited spectral window capacity for multiplex detection and inability to provide absolute quantification of biomolecules. After recalling the sequences of tagging strategies, an apparent question is why not use stable isotopes from the start? A reasonable explanation is the lack of reliable means for accurate and precise quantification of stable isotopes at that time. The situation has changed greatly at present, since several atomic mass spectrometric measures for metal stable isotopes have been developed. Among the newly developed techniques, inductively coupled plasma mass spectrometry is an ideal technique to determine metal stable isotope-tagged biomolecules, for its high sensitivity, wide dynamic linear range, and more importantly multiplex and absolute quantification ability. Since the first published report by our group, metal stable isotope tagging has become a revolutionary technique and gained great success in biomolecule quantification. An exciting research highlight in this area

  12. Biomolecule-assisted synthesis of highly stable dispersions of water-soluble copper nanoparticles. (United States)

    Xiong, Jing; Wu, Xue-dong; Xue, Qun-ji


    Water-soluble and highly stable dispersions of copper nanoparticles were obtained using a biomolecule-assisted synthetic method. Dopamine was utilized as both reducing and capping agent in aqueous medium. The successful formation of DA-stabilized copper particles was demonstrated by ultraviolet-visible spectroscopy (UV-Vis), transmission electron microscopy (TEM), Zeta potential measurement, and Fourier transform infrared spectroscopy (FT-IR). The mechanism of dopamine on the effective reduction and excellent stability of copper nanoparticles was also discussed. This facile biomolecule-assisted technique may provide a useful tool to synthesize other nanoparticles that have potential application in biotechnology.

  13. Study of interactions between cells and microbubbles in high speed centrifugation field for biomolecule delivery. (United States)

    He, Chuan; Chen, Jie


    Biomolecule delivery has a very wide range of applications in biology and medicine. In this study, a microbubble based delivery method was developed. In a high centrifugation field, cells deform and collide with microbubbles to induce intracellular pathways on cell membranes. As a result, biomaterials can then easily enter cells. Experimental results show that this delivery method can achieve high delivery efficiency. Simulation results showed that cells with more deformed structure experienced higher strain on cell membranes than cells with less deformed structure. The models can help explain how centrifugation affects cell membrane permeability. By controlling cell morphology and its mechanical properties, high biomolecule delivery efficiency can be achieved.


    Directory of Open Access Journals (Sweden)

    E. Lazarevich


    Full Text Available The article is present new idea of the creation, developments and improvements of the electronic equipment of complex systems by means of the virtual electronic components. The idea of the virtual electronic components is a presentation and perception of the creation and developments of the equipment on two forming: real – in the manner of standard marketed block of the intellectual property and image – in the manner of virtual component. The real component in most cases slows the development of the electronic equipment. The imaginary component is the «locomotive» of development of the electronic equipment. The Imaginary component contains the scientific has brushed against developer. The scientific has brushed against developer reveals of itself in the manner of virtual component on the modern level of the design rates of microelectronics.

  15. The design and analysis of the main equipment in combination of solar drying system%太阳能组合干燥系统主体设备的设计与分析

    Institute of Scientific and Technical Information of China (English)

    王文茹; 董继先; 李靖


    In this paper,we take the combination of drying equipment for example,For drying non-uniformity problem of the combination drying oven and solar collector selection prob-lem,we analyze the type of solar collector that is used for it,and calculate detailedly the col-lector area,collection of heat energy and recovery period of them.Finally,we optimize the type and structure of the selected collector in Xi′an area,and provide the basis for drying e-quipment to make better use of solar energy.For the unevenness problem in the combination of hot air drying oven.To achieve the uniformity of material drying to further enhance dry material quality and provide help for further study on the combination of drying equipment, we put forward improvement scheme and carry on the reasonable design by changing the way of hot air into the combination for hot air vacuum drying oven.%以太阳能组合干燥设备为例,针对组合干燥箱内热风干燥不均匀性问题以及太阳能集热器的选型问题通过对太阳能集热器的类型进行了分析,详细地计算了它们的集热面积、集热能量以及回收年限,最后优化出在西安地区选用集热器的类型和结构,并通过改变热风进风方式对热风真空组合干燥箱提出改进方案,最终实现物料干燥的均匀性及物料干燥品质进一步提高的目的。优化组合干燥设备为干燥设备更好地利用太阳能及组合干燥设备的进一步研究提供依据。

  16. 卷烟包装设备专用零件参数化CAD系统开发%Development of a parametric designing CAD system for parts special for cigarette packing equipment

    Institute of Scientific and Technical Information of China (English)

    吴旭; 何霄峰; 甘蔚钰


    在Pro/E软件环境下,利用二次开发工具Pro/Tookit结合Pro/E的布局功能开发了一套针对卷烟包装设备的专用零件参数化CAD系统.该系统具有便捷的用户菜单,通过修改布局文件中烟包、烟条规格参数,可以对卷烟包装设备专用零件进行自动参数化设计,并输出相应的三维零件图、三维装配图和二维工程图.同时,系统实现了零件的自动设计和装配检验,大大缩短了产品的开发时间,提高了设计效率.%Based on Pro/Engineering software, a parametric designing CAD system for parts specially used in cigarette packaging equipment had been developed by Pro/Toolkit and the Layout function. A user-friendly menu was provided in the system. The parametric part de-signing for the parts could be conducted by changing the parameters in the layout. And the 3D part models, 3D assembly models and 2D engineering drawings could also be output. Au-tomatic part designing and assembly testing had been realized in the system, which could greatly shorten the product's development time and improve the design quality.

  17. 试验用低温巴氏杀菌设备设计与性能测试%Design and Performance Test of Low-temperature Pasteurization Equipment for Experiment

    Institute of Scientific and Technical Information of China (English)

    刘文营; 王飞; 郭立华; 黄丽燕; 赵维高; 卢晓明


    In order to promote the application of low-temperature pasteurization in the heat thermosensitive materials, the equipment was designed. The design principle, its structural characteristics and processes were proposed, and the thermal power, heat transfer and flow resistance were analyzed. The maximum processing capacity was 13 800 mL/h with 230 mL/min of maximum flow velocity. Taking water as treatment material, the temperature of water can reach to 52℃ from 4℃ in 4. 5 s, and reduce to 20℃ from 52℃ in 3 s or 10t in 6 s. And the bacterium concentration of liquid egg white could lessen to 100 CFU/mL in 2.5 min at 55℃. The performance test shows that the system possesses a good heat-transfer capability, and a high sterilizing effect. Results provide the technical basis for the design and manufacture of low-temperature pasteurization equipment.%设计了一套试验用低温巴氏杀菌设备,阐述了设备的工作原理、主体结构和工作过程,计算了设备的传热能力.依据蠕动泵规格参数,设备最大流量为230 mL/min,最大处理能力为13 800 mL/h.采用水为处理物料,经传热能力计算可知设备满足物料的升温需要,且可以实现在4.5s内从4℃到52℃的升温,以及3 s内52℃到20℃和6s内52℃到10℃的降温.蛋清液杀菌试验表明,在55℃条件下,2.5 min可以实现菌体浓度降为100 CFU/mL.性能试验显示:设备传热能力较强,具有较好的杀菌效果.

  18. Design of Turning Equipment of Niobium Ring-ceramic Matrix Composites Rocket Engine Thrusters%铌环-C/SiC复合材料喷管的车削工装设计

    Institute of Scientific and Technical Information of China (English)

    黎明; 顾力强; 郭洪勤


    A model of rocket engine adopts the ceramic matrix composites nozzle, on which a niobium ring was deposited to connect to the engine by means of electron beam welding. For turning the welding stage of niobium ring, this paper introduces a kind of process equipment design train of thought. By using combining process equipment to find new positioning base, it effectively solved the problem of the fracture of ceramic matrix composites. This technology completely satisfied the requirements of tolerance of welding stage by turning results. And it improves the performance of the engine much further.%某火箭发动机采用 C/SiC复合材料的喷管,并在喷管上沉积一个带焊接头的铌环,通过该铌环与发动机金属头部进行电子束焊接。现需要车削完成喷管铌环的焊接台阶等特征。本文介绍了一种工装设计的新思路,即通过组合车削工装寻找定位基准。通过实际加工证明:该工艺技术完全满足焊接台阶的形位公差要求,并有效地解决了 C/SiC 复合材料脆性断裂等难题,最终满足了发动机的性能指标。


    Institute of Scientific and Technical Information of China (English)

    魏广; 马少君; 陈联; 李元明


    This paper mainly introduced the design of The testing and control apparatus for equipment used in micro-wave-switch’ s longevity validation. The testing and control apparatus can remote control and collect data of microwave-switch,control vacuum pumps and heaters on the equipment,measure the pressure and temperature in the vacuum,and of-fer human-computer interface. The testing and control apparatus can also protect the microwave-switch from fault mode. The central processing unit of the apparatus is the DSP system,which achieve all the Real-time control and testing. The hu-man-computer interface is provided by a Touch Control integrative Machine,which can receive and display the data and save them.%主要介绍了微波开关寿命试验设备中测控仪的设计。测控仪的主要功能是实现对微波开关的控制指令传送、状态遥测参数采集,对试验设备的各种真空泵、加热器进行控制,实现真空室压力、温度的采集与控制,并提供相关的人机交互界面,完成非正常情况的保护处理等。测控仪的设计采用了TI公司的DSP组成核心控制单元,实现参数的实时控制和采集;采用WINCE触控一体机提供人机交互界面,实现通讯数据的接收、显示、及数据存储。

  20. 医疗设备质量检测及风险评估软件的设计与应用%Design and application of a software in medical equipment detecting and risk assessment

    Institute of Scientific and Technical Information of China (English)

    杨俊; 潘其明; 金伟


    Objective: Regulations and requirements of application security and quality assurance will merged into software module design, and then guide and develop the medical equipment detect and risk assessment. Methods: We compiled software by C/S architecture, SQL Server 2000 database, Delphi 7 and windows operating system. Results: It could provide guarantee by the software which is integrated by maintenance, measurement and quality control. Conclusion: The development and application of medical equipment information management system promote the management, construction and improve the security.%目的:将医疗设备应用安全和质量保证有关规定和要求,融入软件系统模块设计中,以此来指导和开展医疗设备质量检测和风险评估工作.方法:采用C/S体系结构,以MS SQL Server 2000作为后台数据库,采用Delphi7作为客户端开发工具,以Windows系统作为操作平台,编制医疗设备质量检测及风险评估软件.结果:将维修、计量以及质量检测整合成为一个软件平台,使管理更加规范.为医院的医疗设备质量检测及风险评估提供了有力的保障.结论:通过对医院医疗仪器设备信息管理系统的开发与应用,推进了医疗设备管理的规范化和标准化,加快了医院信息化建设的步伐,提高了医疗设备的安全性.

  1. The dual aptamer approach: rational design of a high-affinity FAD aptamer. (United States)

    Merkle, T; Holder, I T; Hartig, J S


    A design strategy for high-affinity aptamers of complex biomolecules is presented. We developed an RNA with FAD-binding properties by combining known ATP- and FMN-aptamers. Cooperative binding of FAD was shown by SPR spectroscopy and fluorescence assays. The strategy should be transferable to several other biomolecules.

  2. Carbon Absorber Retrofit Equipment (CARE)

    Energy Technology Data Exchange (ETDEWEB)

    Klein, Eric [Neumann Systems Group, Incorporated, Colorado Springs, CO (United States)


    During Project DE-FE0007528, CARE (Carbon Absorber Retrofit Equipment), Neumann Systems Group (NSG) designed, installed and tested a 0.5MW NeuStream® carbon dioxide (CO2) capture system using the patented NeuStream® absorber equipment and concentrated (6 molal) piperazine (PZ) as the solvent at Colorado Springs Utilities’ (CSU’s) Martin Drake pulverized coal (PC) power plant. The 36 month project included design, build and test phases. The 0.5MW NeuStream® CO2 capture system was successfully tested on flue gas from both coal and natural gas combustion sources and was shown to meet project objectives. Ninety percent CO2 removal was achieved with greater than 95% CO2product purity. The absorbers tested support a 90% reduction in absorber volume compared to packed towers and with an absorber parasitic power of less than 1% when configured for operation with a 550MW coal plant. The preliminary techno-economic analysis (TEA) performed by the Energy and Environmental Research Center (EERC) predicted an over-the-fence cost of $25.73/tonne of CO2 captured from a sub-critical PC plant.

  3. Liquid-Liquid Extraction Equipment

    Energy Technology Data Exchange (ETDEWEB)

    Jack D. Law; Terry A. Todd


    Solvent extraction processing has demonstrated the ability to achieve high decontamination factors for uranium and plutonium while operating at high throughputs. Historical application of solvent extraction contacting equipment implies that for the HA cycle (primary separation of uranium and plutonium from fission products) the equipment of choice is pulse columns. This is likely due to relatively short residence times (as compared to mixer-settlers) and the ability of the columns to tolerate solids in the feed. Savannah River successfully operated the F-Canyon with centrifugal contactors in the HA cycle (which have shorter residence times than columns). All three contactors have been successfully deployed in uranium and plutonium purification cycles. Over the past 20 years, there has been significant development of centrifugal contactor designs and they have become very common for research and development applications. New reprocessing plants are being planned in Russia and China and the United States has done preliminary design studies on future reprocessing plants. The choice of contactors for all of these facilities is yet to be determined.

  4. Design and development of an anthropomorphic phantom equipped with detectors in order to evaluate the effective dose E at workplaces: feasibility study; Conception et developpement d'un fantome anthropomorphe equipe de detecteurs dans le but d'evaluer la dose efficace a un poste de travail: etude de faisabilite

    Energy Technology Data Exchange (ETDEWEB)

    Furstoss, Ch


    My PhD study aims to determine the feasibility to design and develop, for photon fields, an anthropomorphic phantom equipped with detectors in order to evaluate the effective dose E at workplaces. First of all, the energy losses within the organs are calculated using the M.C.N.P.X. Monte Carlo code, in order to determine the detection positions within the different organs. Then, to decrease the number of detection positions, the organ contribution to the effective dose is studied. Finally, the characteristics of the detectors to insert and the characteristics of the phantom to use are deduced. The results show that 24 or 23 detection positions, according to the wT values (publication 60 or new recommendations of the ICRP), give a E estimation with an uncertainty of {+-}15 % from 50 keV to 4 MeV. Moreover, the interest of such an instrument is underlined while comparing the E estimation by the personal dose equivalent Hp to the E estimation by the instrumented phantom when the phantom is irradiated by point sources (worker in front of a glove box for example). Last, after the detector and phantom characteristic determination, two types of detectors and one type of phantom are selected. However, for the detectors mainly, developments are necessary. Follow up this study, the characterization and the adaptation of the detectors to the project would be interesting. Furthermore, the study to mixed photon-neutrons would be required the needs of the radiological protection community. (author)

  5. Design analysis of the molten core confinement within the reactor vessel in the case of severe accidents at nuclear power plants equipped with a reactor of the VVER type (United States)

    Zvonaryov, Yu. A.; Budaev, M. A.; Volchek, A. M.; Gorbaev, V. A.; Zagryazkin, V. N.; Kiselyov, N. P.; Kobzar', V. L.; Konobeev, A. V.; Tsurikov, D. F.


    The present paper reports the results of the preliminary design estimate of the behavior of the core melt in vessels of reactors of the VVER-600 and VVER-1300 types (a standard optimized and informative nuclear power unit based on VVER technology—VVER TOI) in the case of beyond-design-basis severe accidents. The basic processes determining the state of the core melt in the reactor vessel are analyzed. The concept of molten core confinement within the vessel based on the idea of outside cooling is discussed. Basic assumptions and models, as well as the results of calculation of the interaction between molten materials of the core and the wall of the reactor vessel performed by means of the SOCRAT severe accident code, are presented and discussed. On the basis of the data obtained, the requirements on the operation of the safety systems are determined, upon the fulfillment of which there will appear potential prerequisites for implementing the concept of the confinement of the core melt within the reactor in cases of severe accidents at nuclear power plants equipped with VVER reactors.

  6. 基于PDPC-EMV模型的装备研制方案优化设计%Optimization Design for Equipment Development Scheme Based on PDPC-EMV Model

    Institute of Scientific and Technical Information of China (English)



    By the organic integration of PDPC in total quality management system and EMV with decision-mak-ing technique, in this paper, the PDPC-EMV theoretical model was established, and this model was introduced into the design scheme of weapon equipment development as well as it also provided a new ideas for the de-sign cheme. So it solved the problmes exsited in total quality management with the risk assessment method.%将全面质量管理的过程决策程序图法(PDPC)与决策分析技术的期望值法(EMV)有机结合,构建了PDPC-EMV理论模型,并将该模型引入到武器装备研制方案的设计环节中,用风险评估的思维解决全面质量管理的问题,为武器装备研制方案的设计工作开辟了一种先期质量控制思路。

  7. Dairy Equipment Lubrication (United States)


    Lake To Lake Dairy Cooperative, Manitowoc, Wisconsin, operates four plants in Wisconsin for processing milk, butter and cheese products from its 1,300 member farms. The large co-op was able to realize substantial savings by using NASA information for improved efficiency in plant maintenance. Under contract to Marshall Space Flight Center, Midwest Research Institute compiled a handbook consolidating information about commercially available lubricants. The handbook details chemical and physical properties, applications, specifications, test procedures and test data for liquid and solid lubricants. Lake To Lake's plant engineer used the handbook to effect savings in maintenance labor and materials costs by reducing the number of lubricants used on certain equipment. Strict U.S. Department of Agriculture and Food and Drug Administration regulations preclude lubrication changes n production equipment, but the co-op's maintenance chief was able to eliminate seven types of lubricants for ancillary equipment, such as compressors and high pressure pumps. Handbook data enabled him to select comparable but les expensive lubricants in the materials consolidation process, and simplified lubrication schedules and procedures. The handbook is in continuing use as a reference source when a new item of equipment is purchased.

  8. Controlling plasmon coupling in biomolecule-linked metal nanoparticle assemblies (United States)

    Sebba, David S.

    Molecular control of plasmon coupling is investigated in biomolecule-linked nanoparticle assemblies in two-particle, small cluster, and extended network formats. The relationship between structure and optical properties is explored through comparison of measured spectra with simulated spectra calculated using structural models based upon measured structural parameters. A variety of techniques are used to characterize nanoparticle assemblies, including ensemble extinction and elastic scattering spectroscopy, single-assembly scattering spectroscopy, transmission electron microscopy, and dynamic light scattering. Initially, molecular control of plasmon coupling is investigated in ˜100 nm assemblies composed of 13 nm gold "satellite" particles tethered by duplex DNA to a 50 nm gold "core" particle. Comparison of core-satellite assemblies formed with duplex DNA tethers of varying length demonstrates that, while core-satellite separation is controlled by the number of base pairs in the DNA tether, structural properties such as core:satellite ratio and yield are independent of DNA tether length. Thus, plasmon coupling within these assemblies is determined by the number of base pairs in the duplex DNA tether; compact assemblies in which tethers are composed of fewer base pairs exhibit plasmon bands that are red-shifted relative to the bands of extended assemblies, indicating increased plasmon coupling in the compact assemblies. Subsequently, core-satellite assemblies are formed with reconfigurable DNA nanostructure tethers that modulate interparticle separation in response to a molecular stimulus. Assembly reconfiguration from a compact to an extended state results in blue-shifting of the assembly plasmon resonance, indicating reduced interparticle coupling and lengthening of the core-satellite tether. Comparison between measured and simulated spectra revealed a close correspondence and provided validation of the structural models that link assembly plasmonic properties

  9. Design of Bogie Anti-corrosive Thick Coating Intense Radiation Curing Technology and Equipment%浅析转向架防腐厚涂层强辐射固化工艺及设备设计

    Institute of Scientific and Technical Information of China (English)

    于喜年; 赵月红; 刘军


    In order to solve the problem on bogie anti-corrosive thick coating curing,then the thickness of anti-corrosion coating 250 μm or/about and the thickness of general dry film and 5~8 kg limited of scraping putty weight were required in accordance with relevant standards and specifications,as requlated with anti-corrosive thickness.Therefore,the still high quality on welding bogie was proposed and reguested.Analysis of bogie anti-corrosion thick coating paint,curing technology and optimal combination of special anti-corrosive coatings on the surface of high speed bogie was made,which is favourable for tech-equipment and process arrangement and coat infense radiation curing.Short-wave radiation heater is the key component of the coating to be cured.A special combination of structural design by elliptical surface and parabolic surface was adopted.It is more conducive to deep directional radiation which makes the solvent in the bottom of anti-corrosive coating evaporate quickly as a result of strong coating adhesion.Therefore short-wave radiation heater is more suitable for curing the thick coating of wheel axis.Intense radiation curing equipment,parametric design and its virtual assembly provide quick and easy reference in design of anti-corrosive coating curing equipment to produce other relevant products for different manufactures being concerned.%为解决转向架防腐厚涂层固化问题,依照相关标准和规范,对防腐涂层厚度作出规定,要求总干膜厚度为250μm左右,并对腻子的刮涂重量限制在5~8 kg以内,对转向架的焊接质量提出了更高的要求;分析了转向架防腐厚涂层涂覆、固化工艺以及高速动车转向架专用防腐涂料的最优组合,有利于涂层强辐射固化工艺设计和工艺过程安排;短波辐射加热器为涂层固化的关键部件,采用抛物面与椭圆面组合的特殊结构设计,更有利于深层定向辐射,使防腐涂层的底部溶剂迅速挥发,增强了涂层的附

  10. Plasma-based surface modification of polystyrene microtiter plates for covalent immobilization of biomolecules. (United States)

    North, Stella H; Lock, Evgeniya H; Cooper, Candace J; Franek, James B; Taitt, Chris R; Walton, Scott G


    In recent years, polymer surfaces have become increasingly popular for biomolecule attachment because of their relatively low cost and desirable bulk physicochemical characteristics. However, the chemical inertness of some polymer surfaces poses an obstacle to more expansive implementation of polymer materials in bioanalytical applications. We describe use of argon plasma to generate reactive hydroxyl moieties at the surface of polystyrene microtiter plates. The plates are then selectively functionalized with silanes and cross-linkers suitable for the covalent immobilization of biomolecules. This plasma-based method for microtiter plate functionalization was evaluated after each step by X-ray photoelectron spectroscopy, water contact angle analysis, atomic force microscopy, and bioimmobilization efficacy. We further demonstrate that the plasma treatment followed by silane derivatization supports direct, covalent immobilization of biomolecules on microtiter plates and thus overcomes challenging issues typically associated with simple physisorption. Importantly, biomolecules covalently immobilized onto microtiter plates using this plasma-based method retained functionality and demonstrated attachment efficiency comparable to commercial preactivated microtiter plates.

  11. Applying forces to elastic network models of large biomolecules using a haptic feedback device. (United States)

    Stocks, M B; Laycock, S D; Hayward, S


    Elastic network models of biomolecules have proved to be relatively good at predicting global conformational changes particularly in large systems. Software that facilitates rapid and intuitive exploration of conformational change in elastic network models of large biomolecules in response to externally applied forces would therefore be of considerable use, particularly if the forces mimic those that arise in the interaction with a functional ligand. We have developed software that enables a user to apply forces to individual atoms of an elastic network model of a biomolecule through a haptic feedback device or a mouse. With a haptic feedback device the user feels the response to the applied force whilst seeing the biomolecule deform on the screen. Prior to the interactive session normal mode analysis is performed, or pre-calculated normal mode eigenvalues and eigenvectors are loaded. For large molecules this allows the memory and number of calculations to be reduced by employing the idea of the important subspace, a relatively small space of the first M lowest frequency normal mode eigenvectors within which a large proportion of the total fluctuation occurs. Using this approach it was possible to study GroEL on a standard PC as even though only 2.3% of the total number of eigenvectors could be used, they accounted for 50% of the total fluctuation. User testing has shown that the haptic version allows for much more rapid and intuitive exploration of the molecule than the mouse version.

  12. Comparison of various silica-based monoliths for the analysis of large biomolecules. (United States)

    Vuignier, Karine; Fekete, Szabolcs; Carrupt, Pierre-Alain; Veuthey, Jean-Luc; Guillarme, Davy


    In the present study, three types of silica-based monoliths, i.e. the first and second generations of commercial silica monolithic columns and a wide-pore prototype monolith were compared for the analysis of large biomolecules. These molecules possess molecular weights between 1 and 66 kDa. The gradient kinetic performance of the first-generation monolith was lower than that of the second generation, for large biomolecules (>14 kDa) but very close with smaller ones (1.3-5.8 kDa). In contrast, the wide-pore prototype column was particularly attractive with proteins larger than 19 kDa (higher peak capacity). Among these three columns, the selectivity and retention remained quite similar but a possible larger number of accessible and charged residual silanols was noticed on the wide-pore prototype material, which led to unpredicted small changes in selectivity and slightly broader peaks than expected. The peak shapes attained with the addition of 0.1% formic acid in the mobile phase remained acceptable for MS coupling, particularly for biomolecules of less than 6 kDa. It was found that one of the major issues with all of these silica-based monoliths is the possible poor recovery of large biomolecules (principally with monoclonal antibody fragments of more than 25 kDa).

  13. Extracting the Shape and Size of Biomolecules Attached to a Surface as Suspended Discrete Nanoparticles. (United States)

    Milioni, Dimitra; Tsortos, Achilleas; Velez, Marisela; Gizeli, Electra


    The ability to derive information on the conformation of surface attached biomolecules by using simple techniques such as biosensors is currently considered of great importance in the fields of surface science and nanotechnology. Here we present a nanoshape sensitive biosensor where a simple mathematical expression is used to relate acoustic measurements to the geometrical features of a surface-attached biomolecule. The underlying scientific principle is that the acoustic ratio (ΔD/ΔF) is a measure of the hydrodynamic volume of the attached entity, mathematically expressed by its intrinsic viscosity [η]. A methodology is presented in order to produce surfaces with discretely bound biomolecules where their native conformation is maintained. Using DNA anchors we attached a spherical protein (streptavidin) and a rod-shaped DNA (47bp) to a quartz crystal microbalance (QCM) device in a suspended way and predicted correctly through acoustic measurements their conformation, i.e., shape and length. The methodology can be widely applied to draw conclusions on the conformation of any biomolecule or nanoentity upon specific binding on the surface of an acoustic wave device.

  14. Applications of Oblique-Incidence Reflectivity Difference Method in Primary Study of Protein Biomolecules

    Institute of Scientific and Technical Information of China (English)

    ZHANG Hong-Yan; LIANG Ru-Qiang; JIN Kui-Juan; L(U) Hui-Bin; ZHU Xiang-Dong; ZHOU Yue-Liang; RUAN Kang-Cheng; YANG Guo-Zhen


    @@ Oblique-incidence reftectivity difference (OI-RD) analysis is applied to detect the immunoglobulin-G and cytochrome biomolecules on standard glass substrates without fluorescence labelling. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential applications in detection of biochemical processes.

  15. Cellular Viscosity in Prokaryotes and Thermal Stability of Low Molecular Weight Biomolecules. (United States)

    Cuecas, Alba; Cruces, Jorge; Galisteo-López, Juan F; Peng, Xiaojun; Gonzalez, Juan M


    Some low molecular weight biomolecules, i.e., NAD(P)H, are unstable at high temperatures. The use of these biomolecules by thermophilic microorganisms has been scarcely analyzed. Herein, NADH stability has been studied at different temperatures and viscosities. NADH decay increased at increasing temperatures. At increasing viscosities, NADH decay rates decreased. Thus, maintaining relatively high cellular viscosity in cells could result in increased stability of low molecular weight biomolecules (i.e., NADH) at high temperatures, unlike what was previously deduced from studies in diluted water solutions. Cellular viscosity was determined using a fluorescent molecular rotor in various prokaryotes covering the range from 10 to 100°C. Some mesophiles showed the capability of changing cellular viscosity depending on growth temperature. Thermophiles and extreme thermophiles presented a relatively high cellular viscosity, suggesting this strategy as a reasonable mechanism to thrive under these high temperatures. Results substantiate the capability of thermophiles and extreme thermophiles (growth range 50-80°C) to stabilize and use generally considered unstable, universal low molecular weight biomolecules. In addition, this study represents a first report, to our knowledge, on cellular viscosity measurements in prokaryotes and it shows the dependency of prokaryotic cellular viscosity on species and growth temperature.

  16. Probing the interactions between boronic acids and cis-diol-containing biomolecules by affinity capillary electrophoresis. (United States)

    Lü, Chenchen; Li, Hengye; Wang, Heye; Liu, Zhen


    The affinity of boronic acids to cis-diol-containing biomolecules has found wide applications in many fields, such as sensing, separation, drug delivery, and functional materials. A sound understanding of the binding interactions will greatly facilitate exquisite applications of this chemistry. Although a few analytical tools have been available for the characterization of the interactions, these techniques are associated with some apparent drawbacks, so they are only applicable to a limited range of boronic acids and cis-diol-containing biomolecules. Therefore, a widely applicable method is still greatly needed. In this work, an affinity capillary electrophoresis (ACE) method was established and validated to probe the interactions between boronic acids and cis-diol-containing biomolecules. The method was proven to be applicable to almost all types of cis-diol-containing biomolecules and boronic acids. Based on this method, a quantitative, comparative study on the interactions between 14 boronic acids that have important potentials for application with 5 typical monosaccharides of biological importance was carried out. The findings provided new insights into boronate affinity interactions, particularly the relationship between the binding strength with the molecular structures of the binding species. Besides, effects of pH and temperature on the binding strength were also investigated. This method exhibited several significant advantages, including (1) possibility of simultaneous study of multiple interactions, (2) low requirement on the purity of the binding species, (3) wide applicability, and (4) high accuracy and precision.

  17. Systematic methods for defining coarse-grained maps in large biomolecules. (United States)

    Zhang, Zhiyong


    Large biomolecules are involved in many important biological processes. It would be difficult to use large-scale atomistic molecular dynamics (MD) simulations to study the functional motions of these systems because of the computational expense. Therefore various coarse-grained (CG) approaches have attracted rapidly growing interest, which enable simulations of large biomolecules over longer effective timescales than all-atom MD simulations. The first issue in CG modeling is to construct CG maps from atomic structures. In this chapter, we review the recent development of a novel and systematic method for constructing CG representations of arbitrarily complex biomolecules, in order to preserve large-scale and functionally relevant essential dynamics (ED) at the CG level. In this ED-CG scheme, the essential dynamics can be characterized by principal component analysis (PCA) on a structural ensemble, or elastic network model (ENM) of a single atomic structure. Validation and applications of the method cover various biological systems, such as multi-domain proteins, protein complexes, and even biomolecular machines. The results demonstrate that the ED-CG method may serve as a very useful tool for identifying functional dynamics of large biomolecules at the CG level.

  18. Preparation of Biomolecule Microstructures and Microarrays by Thiol–ene Photoimmobilization

    NARCIS (Netherlands)

    Weinrich, Dirk; Köhn, Maja; Jonkheijm, Pascal; Westerlind, Ulrika; Dehmelt, Leif; Engelkamp, Hans; Christianen, Peter C.M.; Kuhlmann, Jürgen; Maan, Jan C.; Nüsse, Dirk; Schröder, Hendrik; Wacker, Ron; Voges, Edgar; Breinbauer, Rolf; Kunz, Horst; Niemeyer, Christof M.; Waldmann, Herbert


    A mild, fast and flexible method for photoimmobilization of biomolecules based on the light-initiated thiol–ene reaction has been developed. After investigation and optimization of various surface materials, surface chemistries and reaction parameters, microstructures and microarrays of biotin, olig

  19. Nanocoating for biomolecule delivery using layer-by-layer self-assembly. (United States)

    Keeney, M; Jiang, X Y; Yamane, M; Lee, M; Goodman, S; Yang, F


    Since its introduction in the early 1990s, layer-by-layer (LbL) self-assembly of films has been widely used in the fields of nanoelectronics, optics, sensors, surface coatings, and controlled drug delivery. The growth of this industry is propelled by the ease of film manufacture, low cost, mild assembly conditions, precise control of coating thickness, and versatility of coating materials. Despite the wealth of research on LbL for biomolecule delivery, clinical translation has been limited and slow. This review provides an overview of methods and mechanisms of loading biomolecules within LbL films and achieving controlled release. In particular, this review highlights recent advances in the development of LbL coatings for the delivery of different types of biomolecules including proteins, polypeptides, DNA, particles and viruses. To address the need for co-delivery of multiple types of biomolecules at different timing, we also review recent advances in incorporating compartmentalization into LbL assembly. Existing obstacles to clinical translation of LbL technologies and enabling technologies for future directions are also discussed.

  20. Prospects of biomolecule sequencing with the techniques of translocation through nanopores: A review

    Energy Technology Data Exchange (ETDEWEB)

    Nosik, V. L., E-mail:; Rudakova, E. B. [Russian Academy of Sciences, Shubnikov Institute of Crystallography (Russian Federation)


    The interest in the functional properties of biomolecules in native solutions (in particular, their interaction with membranes) constantly increases with accumulation of data on the macromolecular structure, obtained by X-ray diffraction (with synchrotron radiation sources), nuclear magnetic resonance, and mass spectrometry; this interest is closely related to the development of new technologies of sequencing (i.e., determining the sequence of nucleotides in DNA biomolecule). One of the most promising 'physical' approaches to sequencing is the application of methods based on the use of nanochannels or nanopores, through which biomolecules pass in ionic solutions under an electric field applied. A nanopore provides spatial localization of molecules and makes it possible to detect a signal (electric, fluorescent, etc.) from an individual nucleotide. In view of the development of new high-intensity pulsed X-ray sources, the popularity of fluorescence analysis constantly increases. The existing methods for simulating the motion of biomolecules and interpreting their structure, sequencing techniques, and the prospects of further development of investigations in this field are discussed.