WorldWideScience

Sample records for biologics license applications

  1. 21 CFR 601.51 - Confidentiality of data and information in applications for biologics licenses.

    Science.gov (United States)

    2010-04-01

    ...) Adverse reaction reports, product experience reports, consumer complaints, and other similar data and... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Confidentiality of data and information in applications for biologics licenses. 601.51 Section 601.51 Food and Drugs FOOD AND DRUG...

  2. 9 CFR 102.3 - License applications.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false License applications. 102.3 Section... AGRICULTURE VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS LICENSES FOR BIOLOGICAL PRODUCTS § 102.3 License applications. (a) U.S. Veterinary Biologics Establishment License. (1)...

  3. A Retrospective Evaluation of the Use of Mass Spectrometry in FDA Biologics License Applications

    Science.gov (United States)

    Rogstad, Sarah; Faustino, Anneliese; Ruth, Ashley; Keire, David; Boyne, Michael; Park, Jun

    2016-11-01

    The characterization sections of biologics license applications (BLAs) approved by the United States Food and Drug Administration (FDA) between 2000 and 2015 were investigated to examine the extent of the use of mass spectrometry. Mass spectrometry was found to be integral to the characterization of these biotherapeutics. Of the 80 electronically submitted monoclonal antibody and protein biotherapeutic BLAs included in this study, 79 were found to use mass spectrometric workflows for protein or impurity characterization. To further examine how MS is being used in successful BLAs, the applications were filtered based on the type and number of quality attributes characterized, the mass spectrometric workflows used (peptide mapping, intact mass analysis, and cleaved glycan analysis), the methods used to introduce the proteins into the gas phase (ESI, MALDI, or LC-ESI), and the specific types of instrumentation used. Analyses were conducted over a time course based on the FDA BLA approval to determine if any trends in utilization could be observed over time. Additionally, the different classes of protein-based biotherapeutics among the approved BLAs were clustered to determine if any trends could be attributed to the specific type of biotherapeutic.

  4. Product Licenses Database Application

    CERN Document Server

    Tonkovikj, Petar

    2016-01-01

    The goal of this project is to organize and centralize the data about software tools available to CERN employees, as well as provide a system that would simplify the license management process by providing information about the available licenses and their expiry dates. The project development process is consisted of two steps: modeling the products (software tools), product licenses, legal agreements and other data related to these entities in a relational database and developing the front-end user interface so that the user can interact with the database. The result is an ASP.NET MVC web application with interactive views for displaying and managing the data in the underlying database.

  5. 21 CFR 601.2 - Applications for biologics licenses; procedures for filing.

    Science.gov (United States)

    2010-04-01

    ... the product for introduction or delivery for introduction into interstate commerce; summaries of...-derived products. (b) (c)(1) To obtain marketing approval for a biological product subject to...

  6. Support for the revocation of general safety test regulations in biologics license applications.

    Science.gov (United States)

    Evans, Dana M; Thorn, Jennifer M; Arch-Douglas, Katherine; Sperry, Justin B; Thompson, Bruce; Davis, Heather L; McCluskie, Michael J

    2016-05-01

    The United States Food and Drug Administration recently removed the requirement for a General Safety Test (GST) for biologics in the Code of Federal Regulations (21 CFR 610.11). The GST, as well as abnormal toxicity (European Pharmacopeia) and innocuity tests (World Health Organization), were designed to test for extraneous toxic contaminants on each product lot intended for human use. Tests require one-week observations for general health and weight following injection of specified volumes of product batches into guinea pigs and mice. At the volumes specified, dose-related toxicity may result when the product is pharmacologically active in rodents. With vaccines, required doses may be > 3 logs higher than intended human dose on a weight-adjusted basis and if an immune modulatory adjuvant is included, systemic immune hyperactivation may cause toxicity. Herein, using the CpG/alum adjuvant combination we evaluated the different test protocols and showed their unsuitability for this adjuvant combination.

  7. 78 FR 78956 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-12-27

    ...), Xiqian (aka Steven) Wei, President, Application Type: New NVO & OFF License Blue Ocean Logistics... Ocean Transportation Intermediary License Applicants The Commission gives notice that the following applicants have filed an application for an Ocean Transportation Intermediary (OTI) license as a...

  8. 76 FR 30360 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-05-25

    ...] [FR Doc No: 2011-12969] FEDERAL MARITIME COMMISSION Ocean Transportation Intermediary License... Commission an application for a license as a Non-Vessel-Operating Common Carrier (NVO) and/or Ocean Freight... applications to amend an existing OTI license or the Qualifying Individual (QI) for a license....

  9. 9 CFR 102.4 - U.S. Veterinary Biologics Establishment License.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false U.S. Veterinary Biologics... LICENSES FOR BIOLOGICAL PRODUCTS § 102.4 U.S. Veterinary Biologics Establishment License. (a) Before a U.S. Veterinary Biologics Establishment License will be issued by the Administrator for any establishment,...

  10. 76 FR 79682 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-12-22

    ... Ocean Transportation Intermediary License; Applicants Notice is hereby given that the following applicants have filed with the Federal Maritime Commission an application for a license as a Non-Vessel...). Notice is also hereby given of the filing of applications to amend an existing OTI license or...

  11. 76 FR 7209 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-02-09

    ... Ocean Transportation Intermediary License; Applicants Notice is hereby given that the following applicants have filed with the Federal Maritime Commission an application for a license as a Non-Vessel...). Notice is also hereby given of the filing of applications to amend an existing OTI license or...

  12. 76 FR 33757 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-06-09

    ... Ocean Transportation Intermediary License; Applicants Notice is hereby given that the following applicants have filed with the Federal Maritime Commission an application for a license as a Non-Vessel...). Notice is also hereby given of the filing of applications to amend an existing OTI license or...

  13. 77 FR 6563 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2012-02-08

    ... Ocean Transportation Intermediary License; Applicants Notice is hereby given that the following applicants have filed with the Federal Maritime Commission an application for a license as a Non-Vessel...). Notice is also hereby given of the filing of applications to amend an existing OTI license or...

  14. 76 FR 76411 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-12-07

    ... Ocean Transportation Intermediary License; Applicants Notice is hereby given that the following applicants have filed with the Federal Maritime Commission an application for a license as a Non-Vessel...). Notice is also hereby given of the filing of applications to amend an existing OTI license or...

  15. 76 FR 62407 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-10-07

    ... Ocean Transportation Intermediary License; Applicants Notice is hereby given that the following applicants have filed with the Federal Maritime Commission an application for a license as a Non-Vessel...). Notice is also hereby given of the filing of applications to amend an existing OTI license or...

  16. 76 FR 78007 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-12-15

    ... Ocean Transportation Intermediary License; Applicants Notice is hereby given that the following applicants have filed with the Federal Maritime Commission an application for a license as a Non-Vessel...). Notice is also hereby given of the filing of applications to amend an existing OTI license or...

  17. 76 FR 28778 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-05-18

    ... Ocean Transportation Intermediary License; Applicants Notice is hereby given that the following applicants have filed with the Federal Maritime Commission an application for a license as a Non-Vessel...). Notice is also hereby given of the filing of applications to amend an existing OTI license or...

  18. 77 FR 1936 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2012-01-12

    ... Ocean Transportation Intermediary License; Applicants Notice is hereby given that the following applicants have filed with the Federal Maritime Commission an application for a license as a Non-Vessel...). Notice is also hereby given of the filing of applications to amend an existing OTI license or...

  19. 76 FR 12962 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2011-03-09

    .../Director, Application Type: New NVO & OFF License. ICT International Cargo Transport (USA) Inc. (NVO & OFF... applicants have filed with the Federal Maritime Commission an application for a license as a Non-Vessel...). Notice is also hereby given of the filing of applications to amend an existing OTI license or...

  20. 78 FR 57633 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-09-19

    ... Ocean Transportation Intermediary License Applicants The Commission gives notice that the following applicants have filed an application for an Ocean Transportation Intermediary (OTI) license as a Non-Vessel... OTI license or the Qualifying Individual (QI) for a licensee. Interested persons may contact...

  1. 78 FR 55698 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-09-11

    ... Ocean Transportation Intermediary License Applicants The Commission gives notice that the following applicants have filed an application for an Ocean Transportation Intermediary (OTI) license as a Non-Vessel... OTI license or the Qualifying Individual (QI) for a licensee. Interested persons may contact...

  2. 77 FR 61752 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2012-10-11

    ... Ocean Transportation Intermediary License Applicants The Commission gives notice that the following applicants have filed an application for an Ocean Transportation Intermediary (OTI) license as a Non-Vessel... existing OTI license or the Qualifying Individual (QI) for a licensee. Interested persons may contact...

  3. 78 FR 27238 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-05-09

    ... Ocean Transportation Intermediary License Applicants The Commission gives notice that the following applicants have filed an application for an Ocean Transportation Intermediary (OTI) license as a Non-Vessel... OTI license or the Qualifying Individual (QI) for a licensee. Interested persons may contact...

  4. 78 FR 40143 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-07-03

    ... Ocean Transportation Intermediary License Applicants The Commission gives notice that the following applicants have filed an application for an Ocean Transportation Intermediary (OTI) license as a Non-Vessel... OTI license or the Qualifying Individual (QI) for a licensee. Interested persons may contact...

  5. 77 FR 39707 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2012-07-05

    ... No: 2012-16473] FEDERAL MARITIME COMMISSION Ocean Transportation Intermediary License; Applicants... application for a license as a Non-Vessel-Operating Common Carrier (NVO) and/or Ocean Freight Forwarder (OFF.... 40101). Notice is also hereby given of the filing of applications to amend an existing OTI license...

  6. 78 FR 20106 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-04-03

    ... Ocean Transportation Intermediary License Applicants The Commission gives notice that the following applicants have filed an application for an Ocean Transportation Intermediary (OTI) license as a Non-Vessel... OTI license or the Qualifying Individual (QI) for a licensee. Interested persons may contact...

  7. 78 FR 43202 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-07-19

    ... Ocean Transportation Intermediary License Applicants The Commission gives notice that the following applicants have filed an application for an Ocean Transportation Intermediary (OTI) license as a Non-Vessel... OTI license or the Qualifying Individual (QI) for a licensee. Interested persons may contact...

  8. 47 CFR 1.83 - Applications for radio operator licenses.

    Science.gov (United States)

    2010-10-01

    ... this chapter. (b) Application filing procedures for commercial radio operator licenses are set forth in... file applications for commercial radio operator licenses is contained in the bulletin “Commercial Radio... 47 Telecommunication 1 2010-10-01 2010-10-01 false Applications for radio operator licenses....

  9. 77 FR 56643 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2012-09-13

    ... Doc No: 2012-22498] FEDERAL MARITIME COMMISSION Ocean Transportation Intermediary License Applicants... Transportation Intermediary (OTI) license as a Non-Vessel-Operating Common Carrier (NVO) and/or Ocean Freight... given of the filing of applications to amend an existing OTI license or the Qualifying Individual...

  10. 9 CFR 102.5 - U.S. Veterinary Biological Product License.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false U.S. Veterinary Biological Product... BIOLOGICAL PRODUCTS § 102.5 U.S. Veterinary Biological Product License. (a) Authorization to produce each biological product shall be specified on a U.S. Veterinary Biological Product License, issued by...

  11. 76 FR 56759 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-09-14

    ... 33171. Officer: Miguel O. Gonzalez, President/Secretary (Qualifying Individual), Application Type: New... Gutierrez, Vice President/Secretary, Application Type: New OFF License. Jacobson Global Logistics, Inc....

  12. 77 FR 60434 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2012-10-03

    ... Type: New OFF License. Farkas Worldwide Shipping Inc. (NVO), 1420 W. Flager Street, Miami, FL 33135, Officers: Heather R. Farkas, President (QI), Maria A. Farkas, Secretary, Application Type: New NVO License... License. Mota Import Export LLC dba MTI Mota Import Export Cargo Express (NVO), 175 Smith Street,...

  13. 78 FR 69852 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-11-21

    ... Proprietor (QI), Application Type: Trade Name Change to Blue Ocean Express. United Harbour Logistics LLC (NVO... Ocean Transportation Intermediary License Applicants The Commission gives notice that the following applicants have filed an application for an Ocean Transportation Intermediary (OTI) license as a...

  14. 76 FR 38651 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-07-01

    ..., (Qualifying Individual), Uri D. Silver, President, Application Type: QI Change. ICT International Cargo... applicants have filed with the Federal Maritime Commission an application for a license as a Non-Vessel...). Notice is also hereby given of the filing of applications to amend an existing OTI license or...

  15. 77 FR 21559 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2012-04-10

    ...: Cintia Altheman, Managing Member (Qualifying Individual). Cristiano de Lima, Managing Member. Application Type: New NVO & OFF License. American Guardship, LLC (NVO & OFF), 6679 Santa Barbara Road, J,...

  16. 75 FR 80499 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-12-22

    ... Street, Orlando, FL 32812. Officers: Tanya Quiroz, Treasurer/ Secretary, (Qualifying Individual), Eddy A. Quiroz, President. Application Type: New NVO License. FPS Logistic (USA) Inc. dba Famous Pacific...

  17. 78 FR 66713 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-11-06

    ..., Baldwin, NY 11510. Officer: Kamran Ali, President (QI), Application Type: New NVO & OFF License. Miragrown... 2D, Philadelphia, PA 19115. Officers: Mykola Chobotar, Chief Executive Manager (QI), James...

  18. 76 FR 16420 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-03-23

    ... Global Logistics Consulting (OFF), 1010 19th Street, 10, Santa Monica, CA 90403. Officer: Andrea Bigi, Sole Proprietor (Qualifying Individual), Application Type: New OFF License. Annex Brands, Inc....

  19. 77 FR 26008 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2012-05-02

    ... License. CLN Worldwide, LLC (NVO & OFF), 1825-C Cross Beam Drive, Charlotte, NC 28217, Officer: David E..., Officers: Robin Lynch, Managing Member (Qualifying Individual), Gina Lynch, Member, Application...

  20. 75 FR 29545 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-05-26

    .... Pacific Coast Highway, Suite 8406, Redondo Beach, CA 90277. Officers: Li Mei, Manager, (Qualifying.../ Treasurer, (Qualifying Individual). Application Type: New NVO License. Dated: May 21, 2010. Karen V....

  1. 76 FR 22104 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2011-04-20

    ... Individual), Application Type: New NVO & OFF License Kinetix International Logistics, LLC (OFF), 5400 Shawnee..., Application Type: Add NVO Service ADM Logistics, Inc. (NVO & OFF), 4666 Faries Parkway, Decatur, IL 62526..., Application Type: New NVO & OFF License Amass International Group (New York) Inc. (NVO), Cargo Bldg. 75,...

  2. 78 FR 48870 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-08-12

    ..., Vice President, Application Type: New OFF License. Supply Chain Shipping LLC (OFF), 4607 44th Street SE..., FL 33126, Officer: Jorge L. Roa, President (QI), Application Type: NVO & OFF License. Kesco Logistics..., Application Type: Add Trade Name Daimon Logistics USA. Logistics International Parcel Shipping Transport...

  3. 21 CFR 601.26 - Reclassification procedures to determine that licensed biological products are safe, effective...

    Science.gov (United States)

    2010-04-01

    ... licensed biological products are safe, effective, and not misbranded under prescribed, recommended, or... Reclassification procedures to determine that licensed biological products are safe, effective, and not misbranded... for the reclassification of all biological products that have been classified into Category IIIA....

  4. 78 FR 48435 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-08-08

    ... 151 Avenue, Pembroke Pines, FL 33027, Officers: Michael L. DeBartolo, Managing Member (QI), Juan D. Restrepo, Member, Application Type: New NVO License. KYS Imports & Exports Inc. (NVO), 1938 Tyler Avenue..., Managing Member (QI), Oscar J. Alzate, Member, Application Type: New OFF License. MGL International...

  5. 75 FR 39529 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-07-09

    ... & NVO License Blue Ocean Freight, Inc. dba Seaship Line (OFF & NVO), 250 Valley Street, 2F, Providence..., Secretary, Application Type: Add NVO Service and Trade Name Change Blue Ocean Shipping, Inc. dba Advanced... Ocean Transportation Intermediary License Applicants Notice is hereby given that the...

  6. 76 FR 10593 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2011-02-25

    ..., Application Type: QI Change. GC Logistics, Inc. (NVO), 79301 Country Club Drive, 104, Bermuda Dunes, CA 92203..., Application Type: New NVO License. KCTC International (America), Inc. dba World Bridge Line dba Green Shipping...: New NVO License. Pegasus3 Worldwide Logistics, LLC (NVO & OFF), 59 Grove Street, Stoughton, MA...

  7. 75 FR 40837 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-07-14

    ...), Virgil Hale, President, Application Type: New OFF License. AFL International Logistics Group LLC (NVO..., Director, Application Type: New OFF License. Direct Service Inc. dba Tiger Freight International... Name Change. Jupiter Airline Services, Inc. dba Mercury Logistics (NVO), 5456 McConnell Avenue,...

  8. 76 FR 554 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2011-01-05

    ... Individual), Oscar Espinosa, President. Application Type: New NVO & OFF License. Frontline Cargo Logistics.... 84th Avenue, Doral, FL 33166. Officers: Manuel J. Rojas, Vice President/Secretary/Treasurer (Qualifying Individual), Annette M. Diaz-Rojas, President. Application Type: License Transfer. Quartz Logistics Inc....

  9. 10 CFR 30.32 - Application for specific licenses.

    Science.gov (United States)

    2010-01-01

    ... MATERIAL Licenses § 30.32 Application for specific licenses. (a) A person may file an application on NRC... maintaining the equipment. (vi) Assessment of releases. A brief description of the methods and equipment to... shutdown. A brief description of the means of restoring the facility to a safe condition after an...

  10. 10 CFR 40.31 - Application for specific licenses.

    Science.gov (United States)

    2010-01-01

    ... Application for specific licenses. (a) A person may file an application for specific license on NRC Form 313... equipment. (vi) Assessment of releases. A brief description of the methods and equipment to assess releases... description of the means of restoring the facility to a safe condition after an accident. (xii)...

  11. 76 FR 3636 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2011-01-20

    ... Ocean Transportation Intermediary License Applicants Notice is hereby given that the following...-Operating Common Carrier (NVO) and/or Ocean Freight Forwarder (OFF)--Ocean Transportation Intermediary (OTI... NVO & OFF License. Marine Cargo Line, L.C. dba, Active Freight & Logistics (NVO), One Blue Hill...

  12. 76 FR 68225 - License Renewal Application for Purdue University

    Science.gov (United States)

    2011-11-03

    ... licensee) to operate the Purdue University Reactor (PUR-1) at a maximum steady-state thermal power of 1... operate PUR-1 up to a steady- state thermal power of 12.5 kW (thermal) for an additional 20 years from the... License No. R- 087 for the PUR-1. Because the license renewal application was filed at least thirty...

  13. INTERNAL HAZARDS ANALYSIS FOR LICENSE APPLICATION

    Energy Technology Data Exchange (ETDEWEB)

    R.J. Garrett

    2005-02-17

    The purpose of this internal hazards analysis is to identify and document the internal hazards and potential initiating events associated with preclosure operations of the repository at Yucca Mountain. Internal hazards are those hazards presented by the operation of the facility and by its associated processes that can potentially lead to a radioactive release or cause a radiological hazard. In contrast to external hazards, internal hazards do not involve natural phenomena and external man-made hazards. This internal hazards analysis was performed in support of the preclosure safety analysis and the License Application for the Yucca Mountain Project. The methodology for this analysis provides a systematic means to identify internal hazards and potential initiating events that may result in a radiological hazard or radiological release during the repository preclosure period. These hazards are documented in tables of potential internal hazards and potential initiating events (Section 6.6) for input to the repository event sequence categorization process. The results of this analysis will undergo further screening and analysis based on the criteria that apply to the performance of event sequence analyses for the repository preclosure period. The evolving design of the repository will be re-evaluated periodically to ensure that internal hazards that have not been previously evaluated are identified.

  14. Nuclear Safety Design Base for License Application

    Energy Technology Data Exchange (ETDEWEB)

    R.J. Garrett

    2005-09-29

    The purpose of this report is to identify and document the nuclear safety design requirements that are specific to structures, systems, and components (SSCs) of the repository that are important to safety (ITS) during the preclosure period and to support the preclosure safety analysis and the license application for the high-level radioactive waste (HLW) repository at Yucca Mountain, Nevada. The scope of this report includes the assignment of nuclear safety design requirements to SSCs that are ITS and does not include the assignment of design requirements to SSCs or natural or engineered barriers that are important to waste isolation (ITWI). These requirements are used as input for the design of the SSCs that are ITS such that the preclosure performance objectives of 10 CFR 63.111(b) [DIRS 173273] are met. The natural or engineered barriers that are important to meeting the postclosure performance objectives of 10 CFR 63.113(b) and (c) [DIRS 173273] are identified as ITWI. Although a structure, system, or component (SSC) that is ITS may also be ITWI, this report is only concerned with providing the nuclear safety requirements for SSCs that are ITS to prevent or mitigate event sequences during the repository preclosure period.

  15. 78 FR 21365 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-04-10

    ... Container Line (NVO & OFF), 510 Plaza Drive, Suite 1210, Atlanta, GA 30349, Officers: John J. Laird... Officer (QI), Rosemary Myers, CEO, Application Type: New NVO & OFF License. Full Hull Logistics, LLC...

  16. 77 FR 45610 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2012-08-01

    ....gov . Access Supply Chain Services LLC (NVO), 65 West 5th Avenue 415, San Mateo, CA 94402, Officer...), Thomas C. Sasser, Treasurer, Application Type: New NVO & OFF License. Green Shipping, Inc. (NVO &...

  17. 76 FR 25690 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2011-05-05

    ... Eastern Express (NVO), 10717 Camino Ruiz, 119, San Diego, CA 92126, Officers: Alex O. De Guzman, President..., (Qualifying Individual), Juan A. Peralta, President, Application Type: New NVO License. Genex Logistics,...

  18. 78 FR 14792 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-03-07

    ..., Secaucus, NJ 07094. Officer: Cesar E. Quinones, Sole Proprietor (QI). Application Type: New NVO License Denizabel Shipping, Inc. (OFF), 19558 NW 50th Court, Miami, FL 33055. Officer: Isabel Ramirez, President...

  19. 77 FR 3773 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2012-01-25

    ... Limited (NVO), F-35/3, Okhla Industrial Area, Phase II, New Delhi 110020 India. Officers: Parveen Mehta... F. Atari, President (Qualifying Individual). Application Type: New NVO & OFF License. Dated:...

  20. 77 FR 35384 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2012-06-13

    ... aka John Chen, President, Application Type: License Transfer JP Express Shipping, Corp. (NVO), 1873... Warehousing, Inc. (NVO & OFF), 1855 Gateway Blvd., 250, Concord, CA 94520, Officers: Steven T. Rubin,...

  1. 75 FR 60124 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-09-29

    ... Global Group, Inc. dba Aerocosta Global Systems Inc. (NVO), 2463 208th Street, 205, Torrance, CA 90501... Proprietor (Qualifying Individual), Application Type: New NVO License Praxis SCM, LLC (NVO & OFF),...

  2. 75 FR 28250 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-05-20

    ...), 8201 NW 66th Street, 6, Miami, FL 33166. Officers: Luis A. Marquez, President, (Qualifying Individual). Maria A. Garcia, Secretary. Application Type: New OFF License. Miriam Family Cargo, Inc. (NVO), 18...

  3. 21 CFR 600.14 - Reporting of biological product deviations by licensed manufacturers.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Reporting of biological product deviations by... HEALTH AND HUMAN SERVICES (CONTINUED) BIOLOGICS BIOLOGICAL PRODUCTS: GENERAL Establishment Standards § 600.14 Reporting of biological product deviations by licensed manufacturers. (a) Who must report...

  4. Requirements for Foreign and Domestic Establishment Registration and Listing for Human Drugs, Including Drugs That Are Regulated Under a Biologics License Application, and Animal Drugs. Final rule.

    Science.gov (United States)

    2016-08-31

    The Food and Drug Administration (FDA) is amending its regulations governing drug establishment registration and drug listing. These amendments reorganize, modify, and clarify current regulations concerning who must register establishments and list human drugs, human drugs that are also biological products, and animal drugs. The final rule requires electronic submission, unless waived in certain circumstances, of registration and listing information. This rulemaking pertains to finished drug products and to active pharmaceutical ingredients (APIs) alone or together with one or more other ingredients. The final rule describes how and when owners or operators of establishments at which drugs are manufactured or processed must register their establishments with FDA and list the drugs they manufacture or process. In addition, the rule makes certain changes to the National Drug Code (NDC) system. We are taking this action to improve management of drug establishment registration and drug listing requirements and make these processes more efficient and effective for industry and for us. This action also supports implementation of the electronic prescribing provisions of the Medicare Prescription Drug, Improvement, and Modernization Act of 2003 (MMA) and the availability of current drug labeling information through DailyMed, a computerized repository of drug information maintained by the National Library of Medicine.

  5. Nuclear Regulatory Commission activities to prepare for reviewing license applications and issuing licenses

    Energy Technology Data Exchange (ETDEWEB)

    Uleck, R.B.; DeFino, C.V. [Nuclear Regulatory Commission, Washington, DC (United States)

    1991-12-31

    The Low-Level Radioactive Waste Policy Amendments Act of 1985 (LLRWPAA) assigned States the responsibility to provide for disposal of commercial low-level radioactive waste (LLRW) by 1993. The LLRWPAA also required the US Nuclear Regulatory Commission (NRC) to establish procedures and develop the technical review capability to process license applications for new LLRW disposal facilities. Under the LLRWPAA, NRC is required, to the extent practicable, to complete its review of an LLRW disposal facility license application within 15 months of its submittal by a State. This provision of the LLRWPAA helps ensure that NRC, in addition to protecting public health and safety and the environment, facilitates States` achievement of LLRWPAA milestones for new facility development. A timely NRC review is needed for States to accomplish their objective of having new disposal facilities in operation on the dates prescribed in the LLRWPAA. To help assure NRC and States` compliance with the provisions of the LLRWPAA, NRC has developed a licensing review strategy that includes: (1) the further development of regulatory guidance, (2) enhancement of licensing review capability, and (3) prelicensing regulatory consultation with potential applicants.

  6. Polythiophenes in biological applications.

    Science.gov (United States)

    Sista, Prakash; Ghosh, Koushik; Martinez, Jennifer S; Rocha, Reginaldo C

    2014-01-01

    Polythiophene and its derivatives have shown tremendous potential for interfacing electrically conducting polymers with biological applications. These semiconducting organic polymers are relatively soft, conduct electrons and ions, have low cytotoxicity, and can undergo facile chemical modifications. In addition, the reduction in electrical impedance of electrodes coated with polythiophenes may prove to be invaluable for a stable and permanent connection between devices and biological tissues. This review article focuses on the synthesis and some key applications of polythiophenes in multidisciplinary areas at the interface with biology. These polymers have shown tremendous potential in biological applications such as diagnostics, therapy, drug delivery, imaging, implant devices and artificial organs.

  7. 77 FR 49445 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2012-08-16

    ..., Officer: Jason P. Kwon, Member (Qualifying Individual), Application Type: New NVO License. Global Supply Chain Solutions Inc. (NVO & OFF), 2301 W. 205th Street, Unit 113, Torrance, CA 90501, Officers: Tony... Secretary (Qualifying Individual), Kurt Keuhn, Treasurer, Application Type: QI Change. UPS Supply...

  8. Residency Applicants Misinterpret Their United States Medical Licensing Exam Scores

    Science.gov (United States)

    Jones, Roger C.; Desbiens, Norman A.

    2009-01-01

    Proper interpretation of the results of the United States Medical Licensing Exam (USMLE) is important for program directors, residents, and faculty who advise applicants about applying for residency positions. We suspected that applicants often misinterpreted their performance in relationship to others who took the same examination. In 2005, 54…

  9. 77 FR 30530 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2012-05-23

    .... Piper, Member/President/CEO (Qualifying Individual), Application Type: New NVO License. Global Wine... NVO Service/Trade Name Change. M & D Global Logistics, Inc. (NVO & OFF), 2211 S. Hacienda Blvd., Suite...), Jun (Alex) Zhong, Secretary, Application Type: Add OFF Service. Metro Freight Services, Inc....

  10. 76 FR 31962 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-06-02

    ..., Application Type: New NVO & OFF. International First Service USA, Inc. dba Global Wine Logistics, Inc. (NVO..., (Qualifying Individual), Mohammad A. Madi, President, Application Type: Add NVO Service. Nano Express Corp... Type: New OFF License. Zai Cargo, Inc. dba Zai Ocean Services dba Zai Container Line (NVO & OFF),...

  11. 76 FR 74060 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2011-11-30

    ..., Application Type: New NVO License. ASF Advantage, L.L.C. (NVO & OFF), 330 Marshall Street, 400, Shreveport, LA..., Application Type: QI Change. Encargo Export Corporation dba Encargo Logistics, dba Encargo Lines (NVO & OFF..., Inc. dba Yacht Exports (NVO), 5337 Orange Drive, Davie, FL 33314; Officer: Alon Ezra,...

  12. 76 FR 9015 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2011-02-16

    .../Treasurer (Qualifying Individual). Application Type: New NVO & OFF. Mega Supply Chain Solutions, Inc. (NVO.... Daley, President. Application Type: New OFF License. American Global Logistics, LLC (NVO & OFF), 53.... dba Citic Global Logistics dba Chimerica Global Logistics (NVO), 817 W. Beverly Blvd., 204,...

  13. 78 FR 37540 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-06-21

    ..., Application Type: New NVO & OFF License. Allyn International Services, Inc. (OFF), 13391 McGregor Blvd., Fort..., Vice President (QI), James A. Williams, CEO, Application Type: QI Change. Thomas Griffin International, Inc. dba Sea Lion Ocean Freight (NVO), 15903 Kent Ct., Tampa, FL 33647, Officer: Thomas L....

  14. 21 CFR 601.25 - Review procedures to determine that licensed biological products are safe, effective, and not...

    Science.gov (United States)

    2010-04-01

    ... biological products are safe, effective, and not misbranded under prescribed, recommended, or suggested... determine that licensed biological products are safe, effective, and not misbranded under prescribed, recommended, or suggested conditions of use. For purposes of reviewing biological products that have...

  15. 21 CFR 515.20 - Approval of medicated feed mill license applications.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Approval of medicated feed mill license... SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS MEDICATED FEED MILL LICENSE Administrative Actions on Licenses § 515.20 Approval of medicated feed mill license applications. Within 90 days after...

  16. 21 CFR 515.21 - Refusal to approve a medicated feed mill license application.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Refusal to approve a medicated feed mill license... SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS MEDICATED FEED MILL LICENSE Administrative Actions on Licenses § 515.21 Refusal to approve a medicated feed mill license application. (a)...

  17. 47 CFR 80.55 - Application for a fleet station license.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Application for a fleet station license. 80.55... SERVICES STATIONS IN THE MARITIME SERVICES Applications and Licenses § 80.55 Application for a fleet... different vessels on the same application. Under these circumstances a fleet station license may be...

  18. 76 FR 72409 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2011-11-23

    ...), Application Type: New OFF License. MBM International Logistics, LLC (NVO & OFF), 650 Atlanta South Parkway... OTI@fmc.gov . AE Eagle Logistics Inc. (NVO & OFF), 765 Route 83, Suite 111, Bensenville, IL 60106... Type: Add OFF Service. Asecomer International Corporation dba Interworld Freight, Inc. dba Junior...

  19. 76 FR 71034 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-11-16

    .... Application Type: Name Change. Abco International Freight (USA) Inc. dba Abco Logistics (NVO), 9420 Telstar...: New NVO & OFF License. Transco International Logistics LLC (OFF), 138-04 175th Street, Jamaica, NY... OTI@fmc.gov . AAB Global Logistics, LLC (NVO & OFF), 201 E. Dundee Road, 2, Palatine, IL...

  20. 77 FR 32010 - Applications (Classification, Advisory, and License) and Documentation

    Science.gov (United States)

    2012-05-31

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Bureau of Industry and Security 15 CFR Part 748 Applications (Classification, Advisory, and License) and Documentation CFR Correction 0 In Title 15 of the Code of Federal Regulations, Parts 300 to 799, revised as...

  1. 75 FR 70673 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-11-18

    ... Ocean Transportation Intermediary License Applicants Notice is hereby given that the following...-Operating Common Carrier (NVO) and/or Ocean Freight Forwarder (OFF)--Ocean Transportation Intermediary (OTI... Line, L.C. dba Active Freight & Logistics (NVO), One Blue Hill Plaza, Pearl River, NY 10965,...

  2. 75 FR 46938 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-08-04

    ... From the Federal Register Online via the Government Publishing Office FEDERAL MARITIME COMMISSION..., Manager, Application Type: New NVO & OFF License. Duke System Logistics Inc. (NVO & OFF), 18645 E. Gale.... Gina Marie Cianelli dba Global Bookings (OFF), 261 Jerry Allen Ridge, Dallas, GA 30132, Officer: Gina...

  3. 76 FR 55909 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-09-09

    ... Global Logistics (Georgia) Inc. dba CTS Global Supply Chain Solutions (NVO & OFF), 5192 Southridge... Application Type: New OFF & NVO License. AA Equipment and Sales, LLC dba AIM Global Logistics (NVO & OFF... Change. BestOcean Worldwide Logistics, Inc. (NVO & OFF), 1300 Valley Vista Drive, Suite 203, Diamond...

  4. 77 FR 59193 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2012-09-26

    .... CTS Global Logistics (Georgia) Inc. dba CTS Global Supply Chain Solutions (NVO & OFF), 5192 Southridge.... Rivera, President (QI), Howard Helfer, Vice President, Application Type: New OFF License. Bring Logistics... Logistics, LLC (NVO & OFF), 4911 N. Portland Avenue, Suite 200, Oklahoma City, OK 73112, Officers: Thomas...

  5. Licensed reactor nuclear safety criteria applicable to DOE reactors

    Energy Technology Data Exchange (ETDEWEB)

    1993-11-01

    This document is a compilation and source list of nuclear safety criteria that the Nuclear Regulatory Commission (NRC) applies to licensed reactors; it can be used by DOE and DOE contractors to identify NRC criteria to be evaluated for application to the DOE reactors under their cognizance. The criteria listed are those that are applied to the areas of nuclear safety addressed in the safety analysis report of a licensed reactor. They are derived from federal regulations, USNRC regulatory guides, Standard Review Plan (SRP) branch technical positions and appendices, and industry codes and standards.

  6. 21 CFR 515.25 - Revocation of order refusing to approve a medicated feed mill license application or suspending...

    Science.gov (United States)

    2010-04-01

    ... medicated feed mill license application or suspending or revoking a license. 515.25 Section 515.25 Food and..., FEEDS, AND RELATED PRODUCTS MEDICATED FEED MILL LICENSE Administrative Actions on Licenses § 515.25 Revocation of order refusing to approve a medicated feed mill license application or suspending or revoking...

  7. 21 CFR 515.11 - Supplemental medicated feed mill license applications.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Supplemental medicated feed mill license... SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS MEDICATED FEED MILL LICENSE Applications § 515.11 Supplemental medicated feed mill license applications. (a) After approval of a medicated...

  8. Biological applications of nanobiotechnology.

    Science.gov (United States)

    de Morais, Michele Greque; Martins, Vilásia Guimarães; Steffens, Daniela; Pranke, Patricia; da Costa, Jorge Alberto Vieira

    2014-01-01

    Nanotechnology is a multidisciplinary field that covers a vast and diverse array of devices derived from engineering, physics, chemistry, and biology. Nanotechnology has opened up by rapid advances in science and technology, creating new opportunities for advances in the fields of medicine, electronics, foods, and the environment. Nanoscale structures and materials (nanoparticles, nanowires, nanofibers, nanotubes) have been explored in many biological applications (biosensing, biological separation, molecular imaging, anticancer therapy) because their novel properties and functions differ drastically from their bulk counterparts. Their high volume/surface ratio, improved solubility, and multifunctionality open many new possibilities. The objective of this review is to describe the potential benefits and impacts of the nanobiotechnology in different areas.

  9. Licensed reactor nuclear safety criteria applicable to DOE reactors

    Energy Technology Data Exchange (ETDEWEB)

    1991-04-01

    The Department of Energy (DOE) Order DOE 5480.6, Safety of Department of Energy-Owned Nuclear Reactors, establishes reactor safety requirements to assure that reactors are sited, designed, constructed, modified, operated, maintained, and decommissioned in a manner that adequately protects health and safety and is in accordance with uniform standards, guides, and codes which are consistent with those applied to comparable licensed reactors. This document identifies nuclear safety criteria applied to NRC (Nuclear Regulatory Commission) licensed reactors. The titles of the chapters and sections of USNRC Regulatory Guide 1.70, Standard Format and Content of Safety Analysis Reports for Nuclear Power Plants, Rev. 3, are used as the format for compiling the NRC criteria applied to the various areas of nuclear safety addressed in a safety analysis report for a nuclear reactor. In each section the criteria are compiled in four groups: (1) Code of Federal Regulations, (2) US NRC Regulatory Guides, SRP Branch Technical Positions and Appendices, (3) Codes and Standards, and (4) Supplemental Information. The degree of application of these criteria to a DOE-owned reactor, consistent with their application to comparable licensed reactors, must be determined by the DOE and DOE contractor.

  10. 47 CFR 13.13 - Application for a renewed or modified license.

    Science.gov (United States)

    2010-10-01

    .... 13.13 Section 13.13 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL COMMERCIAL RADIO... a period of 1 year. (d) Provided that a person's commercial radio operator license was not revoked... License, or GMDSS Radio Operator/Maintainer license, who has an application for another commercial...

  11. 78 FR 21930 - Aquenergy Systems, Inc.; Notice of Intent To File License Application, Filing of Pre-Application...

    Science.gov (United States)

    2013-04-12

    ... Energy Regulatory Commission Aquenergy Systems, Inc.; Notice of Intent To File License Application... Filing: Notice of Intent to File License Application and Request to Use the Traditional Licensing Process. b. Project No.: P-2428-004. c. Date Filed: November 11, 2012. d. Submitted by: Aquenergy...

  12. Semiconductor nanostructures in biological applications

    Energy Technology Data Exchange (ETDEWEB)

    Alexson, Dimitri [Department of Electrical and Computer Engineering, University of Illinois at Chicago, Chicago, IL 60607 (United States); Chen Hongfeng [Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607 (United States); Cho, Michael [Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607 (United States); Department of Physics, University of Illinois at Chicago, Chicago, IL 60607 (United States); Dutta, Mitra [Department of Electrical and Computer Engineering, University of Illinois at Chicago, Chicago, IL 60607 (United States); Department of Physics, University of Illinois at Chicago, Chicago, IL 60607 (United States); Li Yang [Department of Electrical and Computer Engineering, University of Illinois at Chicago, Chicago, IL 60607 (United States); Shi, Peng [Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607 (United States); Raichura, Amit [Department of Electrical and Computer Engineering, University of Illinois at Chicago, Chicago, IL 60607 (United States); Ramadurai, Dinakar [Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607 (United States); Parikh, Shaunak [Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607 (United States); Stroscio, Michael A [Department of Electrical and Computer Engineering, University of Illinois at Chicago, Chicago, IL 60607 (United States); Department of Physics, University of Illinois at Chicago, Chicago, IL 60607 (United States); Vasudev, Milana [Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607 (United States)

    2005-07-06

    Semiconductor nanostructures in biological applications are discussed. Results are presented on the use of colloidal semiconductor quantum dots both as biological tags and as structures that interact with and influence biomolecules. Results are presented on the use of semiconducting carbon nanotubes in biological applications. (topical review)

  13. 76 FR 72984 - Revised Application for a License To Export High-Enriched Uranium

    Science.gov (United States)

    2011-11-28

    ... COMMISSION Revised Application for a License To Export High-Enriched Uranium The application for a license to export high-enriched Uranium has been revised as noted below. Notice of this application was previously... kilograms To fabricate fuel France. Security Complex; October 18, Uranium (93.35%). uranium (174.0...

  14. 47 CFR 0.483 - Applications for amateur or commercial radio operator licenses.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Applications for amateur or commercial radio..., and for Taking Examinations § 0.483 Applications for amateur or commercial radio operator licenses. (a...) Application filing procedures for commercial radio operator licenses are set forth in part 13 of this...

  15. 78 FR 36014 - Deepwater Port License Application: Liberty Natural Gas, LLC, Port Ambrose Deepwater Port

    Science.gov (United States)

    2013-06-14

    ... an application for the licensing of a liquefied natural gas deepwater port and that the application..., and operate a liquefied natural gas (LNG) deepwater port, known as Port Ambrose, located in the New... Maritime Administration Deepwater Port License Application: Liberty Natural Gas, LLC, Port...

  16. 76 FR 71082 - Strata Energy, Inc., Ross Uranium Recovery Project; New Source Material License Application...

    Science.gov (United States)

    2011-11-16

    ... COMMISSION Strata Energy, Inc., Ross Uranium Recovery Project; New Source Material License Application...: Strata Energy, Inc. (Strata) submitted an application for a new source material license for the Ross... Ross Uranium Recovery Project, as required by Title 10 of the Code of Federal Regulations (10 CFR)...

  17. 76 FR 65544 - Standard Format and Content of License Applications for Mixed Oxide Fuel Fabrication Facilities

    Science.gov (United States)

    2011-10-21

    ... issuance of the guide (74 FR 36780). The comment period closed on September 21, 2009. The staff's responses... COMMISSION Standard Format and Content of License Applications for Mixed Oxide Fuel Fabrication Facilities... Format and Content of License Applications for Mixed Oxide Fuel Fabrication Facilities.'' This...

  18. Total System Performance Assessment-License Application Methods and Approach

    Energy Technology Data Exchange (ETDEWEB)

    J. McNeish

    2002-09-13

    ''Total System Performance Assessment-License Application (TSPA-LA) Methods and Approach'' provides the top-level method and approach for conducting the TSPA-LA model development and analyses. The method and approach is responsive to the criteria set forth in Total System Performance Assessment Integration (TSPAI) Key Technical Issue (KTI) agreements, the ''Yucca Mountain Review Plan'' (CNWRA 2002 [158449]), and 10 CFR Part 63. This introductory section provides an overview of the TSPA-LA, the projected TSPA-LA documentation structure, and the goals of the document. It also provides a brief discussion of the regulatory framework, the approach to risk management of the development and analysis of the model, and the overall organization of the document. The section closes with some important conventions that are utilized in this document.

  19. 77 FR 16278 - License Renewal Application for Indian Point Nuclear Generating Units 2 and 3; Entergy Nuclear...

    Science.gov (United States)

    2012-03-20

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION License Renewal Application for Indian Point Nuclear Generating Units 2 and 3; Entergy Nuclear Operations, Inc. AGENCY: Nuclear Regulatory Commission. ACTION: License renewal application; intent...

  20. License Application Design Selection Report, REV 01. August 1999

    Energy Technology Data Exchange (ETDEWEB)

    Hastings, C.R.

    1999-08-30

    In December 1998, the U.S. Department of Energy (DOE) published the ''Viability Assessment of a Repository at Yucca Mountain'' (DOE 1998b). The Viability Assessment described a preliminary design of a potential repository at Yucca Mountain, Nevada, for disposal of spent nuclear fuel and high-level radioactive waste, and assessed the probable behavior of that repository design in the Yucca Mountain geologic setting. The report concluded that 'Yucca Mountain remains a promising site for a geologic repository and that work should proceed to support a decision in 2001 on whether to recommend the site to the President for development as a repository'. It also concluded that 'uncertainties remain about key natural processes, the preliminary design, and how the site and design would interact'. Recognizing that the design that was evaluated will be refined before a license application could be submitted, the Viability Aassesment notes that 'DOE is evaluating several design options and alternatives that could reduce existing uncertainty and improve the performance of the repository system'. During the preparation of the Viability Assessment, DOE asked the contractor for the Civilian Radioactive Waste Management Program to study alternative design concepts for a potential geologic repository for high-level radioactive waste at Yucca Mountain. The License Application Design Selection (LADS) project was initiated to conduct that study. The goal of the project was to develop and evaluate a diverse range of conceptual repository designs that work well in concert with the Yucca Mountain site and to recommend an initial design concept for the possible Site Recommendation and License Apllication. This report presents the results of the LADS project. The design process consisted of two phases. In Phase I, a series of basic design concepts (design alternatives) and components (design features) were analyzed for their potential value

  1. Guidelines for preparing and reviewing applications for the licensing of non-power reactors: Standard review plan and acceptance criteria. NUREG - 1537, Part 2

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-02-01

    NUREG - 1537, Part 2 gives guidance on the conduct of licensing action reviews to NRC staff who review non-power reactor licensing applications. These licensing actions include construction permits and initial operating licenses, license renewals, amendments, conversions from highly enriched uranium to low-enriched uranium, decommissioning, and license termination.

  2. 77 FR 22808 - Biweekly Notice; Applications and Amendments to Facility Operating Licenses and Combined Licenses...

    Science.gov (United States)

    2012-04-17

    ... different type of equipment will be installed) or changes in methods governing normal plant operation. The... governing normal plant operation. In addition, the changes do not impose any new or different requirements...-related system performs its function. The change revises license renewal commitments and aging...

  3. 78 FR 70589 - Biweekly Notice; Applications and Amendments to Facility Operating Licenses and Combined Licenses...

    Science.gov (United States)

    2013-11-26

    ... Oconee assumes 5 gpm back-leakage from the Reactor Building sump to the BWST. The automatic isolation... National Fire Protection Association (NFPA) 805, ``Performance- Based Standard for Fire Protection for... new fire protection licensing basis which complies with the requirements in 10 CFR 50.48(a), 10 CFR...

  4. 76 FR 78702 - Progress Energy Florida, Inc. (Combined License Application for Levy County Nuclear Power Plant...

    Science.gov (United States)

    2011-12-19

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Progress Energy Florida, Inc. (Combined License Application for Levy County Nuclear Power Plant...) and 2.321(b), the Atomic Safety and Licensing Board (Board) in the above-captioned Progress...

  5. 77 FR 61584 - FFP Missouri 12, LLC; Notice of Intent To File License Application, Filing of Pre-Application...

    Science.gov (United States)

    2012-10-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission FFP Missouri 12, LLC; Notice of Intent To File License Application, Filing of Pre-Application Document, and Approving Use of the Traditional Licensing Process a. Type of...

  6. Guidelines for preparing and reviewing applications for the licensing of non-power reactors: Format and Content. NUREG-1537, Part 1

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-02-01

    NUREG - 1537, Part 1 gives guidance to non-power reactor licensees and applicants on the format and content of applications to the Nuclear Regulatory Commission for licensing actions. These licensing actions include construction permits and initial operating licenses, license renewals, amendments, conversions from highly enriched uranium to low-enriched uranium, decommissioning, and license termination.

  7. 78 FR 73566 - Standard Format and Content for a License Application for an Independent Spent Fuel Storage...

    Science.gov (United States)

    2013-12-06

    ... COMMISSION Standard Format and Content for a License Application for an Independent Spent Fuel Storage... public comment draft regulatory guide (DG), DG-3042, ``Standard Format and Content for a License... Waste'' (Ref. 1), Subpart B, ``License Application, Form, and Contents,'' specifies the information...

  8. Automated License Plate Recognition for Toll Booth Application

    Directory of Open Access Journals (Sweden)

    Ketan S. Shevale

    2014-10-01

    Full Text Available This paper describes the Smart Vehicle Screening System, which can be installed into a tollbooth for automated recognition of vehicle license plate information using a photograph of a vehicle. An automated system could then be implemented to control the payment of fees, parking areas, highways, bridges or tunnels, etc. There are considered an approach to identify vehicle through recognizing of it license plate using image fusion, neural networks and threshold techniques as well as some experimental results to recognize the license plate successfully.

  9. Synthetic biology for therapeutic applications.

    Science.gov (United States)

    Abil, Zhanar; Xiong, Xiong; Zhao, Huimin

    2015-02-02

    Synthetic biology is a relatively new field with the key aim of designing and constructing biological systems with novel functionalities. Today, synthetic biology devices are making their first steps in contributing new solutions to a number of biomedical challenges, such as emerging bacterial antibiotic resistance and cancer therapy. This review discusses some synthetic biology approaches and applications that were recently used in disease mechanism investigation and disease modeling, drug discovery and production, as well as vaccine development and treatment of infectious diseases, cancer, and metabolic disorders.

  10. Total System Performance Assessment - License Application Methods and Approach

    Energy Technology Data Exchange (ETDEWEB)

    J. McNeish

    2003-12-08

    ''Total System Performance Assessment-License Application (TSPA-LA) Methods and Approach'' provides the top-level method and approach for conducting the TSPA-LA model development and analyses. The method and approach is responsive to the criteria set forth in Total System Performance Assessment Integration (TSPAI) Key Technical Issues (KTIs) identified in agreements with the U.S. Nuclear Regulatory Commission, the ''Yucca Mountain Review Plan'' (YMRP), ''Final Report'' (NRC 2003 [163274]), and the NRC final rule 10 CFR Part 63 (NRC 2002 [156605]). This introductory section provides an overview of the TSPA-LA, the projected TSPA-LA documentation structure, and the goals of the document. It also provides a brief discussion of the regulatory framework, the approach to risk management of the development and analysis of the model, and the overall organization of the document. The section closes with some important conventions that are used in this document.

  11. 78 FR 38336 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-06-26

    ... Express, LLC (NVO & OFF), 812 Downtowner Blvd., Suite K, Mobile, AL 36609, Officers: Thomas (Mac) H. Mc... License. Magnum-Ramstr Cargo LLC (NVO & OFF), 2 Ethel Road, Suite 202C, Edison, NJ 08817,...

  12. 77 FR 13604 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2012-03-07

    ... 149 Court, Miami, FL 33194, Officer: Robert Esquivel, President/Secretary/Treasurer (Qualifying... Type: New NVO & OFF License. Dated: March 2, 2012. Karen V. Gregory, Secretary. BILLING CODE 6730-01-P...

  13. 75 FR 22408 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-04-28

    ... License. Damco USA Inc. dba Damco Sea and Air dba Damco dba Damco Maritime dba DSL Star Express dba Maersk... Logistics LLC dba JSK Lines (OFF & NVO), 4 Wernik Place, Metuchen, NJ 08840. Officers: Norbert G....

  14. 7 CFR 1530.104 - Application for a license.

    Science.gov (United States)

    2010-01-01

    ... any wholly-owned subsidiary(s) and plant(s) in the case of a consolidated license, and including those... affiliated with any other licensee, or explaining any affiliations, should they exist. (b) A...

  15. 78 FR 36641 - Proposed Information Collection (Application for Approval of a Licensing or Certification and...

    Science.gov (United States)

    2013-06-18

    ... AFFAIRS Proposed Information Collection (Application for Approval of a Licensing or Certification and Organization or Entity) Activity: Comment Request AGENCY: Veterans Benefits Administration, Department of Veterans Affairs. ACTION: Notice. SUMMARY: The Veterans Benefits Administration (VBA), Department...

  16. 76 FR 67557 - Proposed Information Collection (Application for Reimbursement of Licensing or Certification Test...

    Science.gov (United States)

    2011-11-01

    ... AFFAIRS Proposed Information Collection (Application for Reimbursement of Licensing or Certification Test Fees) Activity: Comment Request AGENCY: Veterans Benefits Administration, Department of Veterans Affairs. ACTION: Notice. SUMMARY: The Veterans Benefits Administration (VBA), Department of...

  17. 75 FR 47680 - Proposed Information Collection (Application for Approval of a Licensing or Certification and...

    Science.gov (United States)

    2010-08-06

    ... AFFAIRS Proposed Information Collection (Application for Approval of a Licensing or Certification and Organization or Entity) Activity: Comment Request AGENCY: Veterans Benefits Administration, Department of Veterans Affairs. ACTION: Notice. SUMMARY: The Veterans Benefits Administration (VBA), Department...

  18. 78 FR 40519 - Cooper Nuclear Station; Application and Amendment to Facility Operating License Involving...

    Science.gov (United States)

    2013-07-05

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Cooper Nuclear Station; Application and Amendment to Facility Operating License Involving Proposed... No. DPR-46, issued to Nebraska Public Power District (the licensee), for operation of the...

  19. Overview of the earth mounded concrete bunker prototype license application project: Objectives and approach

    Energy Technology Data Exchange (ETDEWEB)

    Conner, J.E. [EG and G Idaho, Inc., Idaho Falls, ID (United States). Idaho National Engineering Lab.

    1989-11-01

    This paper presents an overview of the objectives and approach taken in developing the Earth-mounded Concrete Bunker Prototype License Application Project. The Prototype License Application Project was initiated by the Department of Energy`s National Low-Level Waste Management Program in early 1987 and completed in November 1988. As part of this project a prototype safety analysis report was developed. The safety analysis report evaluates the licensibility of an earth-mounded concrete bunker for a low-level radioactive waste (LLW) disposal facility located on a hypothetical site in the northeastern United States. The project required approximately five person-years and twenty months to develop.

  20. Environmental Standard Review Plan for the review of license renewal applications for nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    O' Brien, J.; Kim, T.J.; Reynolds, S.

    1991-08-01

    The Environmental Standard Review Plan for the Review of License Applications for Nuclear Power Plants (ESRP-LR) is to be used by the NRC staff when performing environmental reviews of applications for the renewal of power reactor licenses. The use of the ESRP-LR provides a framework for the staff to determine whether or not environmental issues important to license renewal have been identified and the impacts evaluated and provides acceptance standards to help the reviewers comply with the National Environmental Policy Act.

  1. Application of IC Card License for Road Transportation in Commercial Vehicles Supervision and Service

    Directory of Open Access Journals (Sweden)

    Li Weiwei

    2016-01-01

    Full Text Available IC card electronic license for road transport includes the IC card commercial vehicle’s certificate and IC card practitioner’s qualification certificate. In China, the IC card electronic license for road transport is the electronic ID card which must be carried by each commercial vehicles and practitioners. This paper briefly introduces the basic situation, data format and security keys architecture of IC card electronic license for road transportation of China. In order to strengthen the supervision and service of commercial vehicles, this paper puts forward the overall application framework of IC card electronic license for road transport. The application examples of IC card license in the supervision of passenger station, dangerous goods transport management, governance overload and logistics park and port area management are discussed. The practical application results show that the application of IC card electronic license for road transport is an important technical means to improve the supervision ability and service quality of the road transportation industry.

  2. 21 CFR 515.10 - Medicated feed mill license applications.

    Science.gov (United States)

    2010-04-01

    ... Veterinary Medicine home page at http://www.fda.gov/cvm. (b) A completed medicated feed mill license must... certification that the facility will establish and maintain all records required by regulation or order issued... verification of such records. (7) A commitment that current approved or index listed Type B and/or Type...

  3. 77 FR 18815 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2012-03-28

    ... License. Esko, Inc. (NVO), 19008 Herb Court, Rowland Heights, CA 91748. Officers: Lin L. Chen, Vice..., Torrance, CA 90505. Officers: Emily Chen, President (Qualifying Individual). Chun-Yi Lin, Member..., Mobile, AL 36602. Officers: Steven A. Haverstock, President (Qualifying Individual). Kevin L....

  4. 76 FR 39922 - Notice of Acceptance of Application for Special Nuclear Materials License From Sensor Concepts...

    Science.gov (United States)

    2011-07-07

    ... COMMISSION Notice of Acceptance of Application for Special Nuclear Materials License From Sensor Concepts and... Sensor Concepts and Applications, Inc's., (SCA) application is available electronically under ADAMS... Detection Office (DNDO) of the U.S. Department of Homeland Security (DHS). Sensor Concepts and...

  5. 78 FR 75386 - Entergy Operations, Inc.; Combined License Application for River Bend Station Unit 3, Exemption...

    Science.gov (United States)

    2013-12-11

    ... Register on November 3, 2011 (76 FR 72560). Part 50, Appendix E, Section I.5 gives those COL applicants... Preparedness (EP) rules in their Combined License (COL) application. The NRC staff reviewed this request and... submitted prior to, or coincident with, requesting the NRC to resume its review of the COL application,...

  6. 75 FR 57820 - Luminant Generation Company, LLC.; Combined License Application for Comanche Peak Nuclear Power...

    Science.gov (United States)

    2010-09-22

    ..., Combined License (COL) Application, Docket Numbers 52-034 and 52-035, submitted by Luminant Generation... of a COL application until the NRC makes a finding under 10 CFR 52.103(g) pertaining to facility... Analysis Report (FSAR). The proposed exemption would allow Luminant to submit its COL application...

  7. [Medical applications of systems biology].

    Science.gov (United States)

    Demongeot, Jacques

    2009-01-01

    We illustrate in this review some applications of systems biology in the medical and biological areas. After a brief summary of time scales experienced by medical -observations and of the general scheme of dynamic systems, we describe how some techniques underlying the complex systems theory can be applied to model medical issues in immunology, medical genetics, developmental morphogenesis, biochemistry, epidemiology, telemedecine and multiple platforms of expertise. In concluding, we will discuss the issue of "clinomics" coupling clinical and -omics data in a unique patient-specific file.

  8. The contractor`s role in low-level waste disposal facility application review and licensing

    Energy Technology Data Exchange (ETDEWEB)

    Serie, P.J.; Dressen, A.L. [Environmental Issues Management, Inc., Seattle, WA (United States)

    1991-12-31

    The California Department of Health Services will soon reach a licensing decision on the proposed Ward Valley low-level radioactive waste disposal facility. As the first regulatory agency in the country to address the 10 CFR Part 61 requirements for a new disposal facility, California`s program has broken new ground in its approach. Throughout the review process, the Department has relied on contractor support to augment its technical and administrative staff. A team consisting of Roy F. Weston, Inc., supported by ERM-Program Management Corp., Environmental Issues Management, Inc., and Rogers and Associates Engineering Corporation, has worked closely with the Department in a staff extension role. The authors have been involved with the project in contractor project management roles since 1987, and continue to support the Department`s program as it proceeds to finalize its licensing process. This paper describes the selection process used to identify a contractor team with the needed skills and experience, and the makeup of team capabilities. It outlines the management, communication, and technical approaches used to assure a smooth agency-contractor function and relationship. It describes the techniques used to ensure that decisions and documents represented the Department credibly in its role as the regulatory and licensing agency under the Nuclear Regulatory Commission (NRC) Agreement State program. The paper outlines the license application review process and activities, through preparation of licensing documentation and responses to public comments. Lessons learned in coordination of an agency-contractor team effort to review and license a low-level waste disposal facility are reviewed and suggestions made for approaching a similar license application review and licensing situation.

  9. 75 FR 57267 - Haida Energy, Inc.; Notice of Application for Amendment of License and Soliciting Comments...

    Science.gov (United States)

    2010-09-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Haida Energy, Inc.; Notice of Application for Amendment of License and.... Applicant: Haida Energy, Inc. e. Name of Project: Reynolds Creek Project. f. Location: On Reynolds...

  10. 78 FR 79506 - Tennessee Valley Authority: Exemption From Requirements To Revise Combined License Application To...

    Science.gov (United States)

    2013-12-30

    ... revise their COL application to comply with the EP rules published in the Federal Register (76 FR 72560... revise their combined license (COL) application in order to address enhancements to the Emergency Preparedness (EP) rules at the same time as requesting the NRC to resume the review of their COL...

  11. 76 FR 800 - Ameren Missouri, Combined License Application for Callaway Plant Unit 2; Environmental Assessment...

    Science.gov (United States)

    2011-01-06

    ...) for the Callaway Plant (Callaway), Unit 2, Combined License (COL) Application, Docket Number 52-037... located in Callaway ] County, Missouri. The NRC's review activities relating to the Callaway, Unit 2, COL... adjudicatory proceedings related to the Callaway, Unit 2, COL application were terminated by the Atomic...

  12. 75 FR 37471 - Biweekly Notice; Applications and Amendments to Facility Operating Licenses Involving No...

    Science.gov (United States)

    2010-06-29

    ... license amendment application, see the application for amendment which is available for public inspection..., which include the cooling water, starting air, lubrication, intake and exhaust, and fuel oil systems... boiling (DNBR) limits, heat flux hot channel factor (F Q (Z)) limits, nuclear enthalpy rise hot...

  13. Application of probabilistic risk assessment in nuclear and environmental licensing processes of nuclear reactors in Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Mata, Jonatas F.C. da; Vasconcelos, Vanderley de; Mesquita, Amir Z., E-mail: jonatasfmata@yahoo.com.br, E-mail: vasconv@cdtn.br, E-mail: amir@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)

    2015-07-01

    The nuclear accident at Fukushima Daiichi, occurred in Japan in 2011, brought reflections, worldwide, on the management of nuclear and environmental licensing processes of existing nuclear reactors. One of the key lessons learned in this matter, is that the studies of Probabilistic Safety Assessment and Severe Accidents are becoming essential, even in the early stage of a nuclear development project. In Brazil, Brazilian Nuclear Energy Commission, CNEN, conducts the nuclear licensing. The organism responsible for the environmental licensing is Brazilian Institute of Environment and Renewable Natural Resources, IBAMA. In the scope of the licensing processes of these two institutions, the safety analysis is essentially deterministic, complemented by probabilistic studies. The Probabilistic Safety Assessment (PSA) is the study performed to evaluate the behavior of the nuclear reactor in a sequence of events that may lead to the melting of its core. It includes both probability and consequence estimation of these events, which are called Severe Accidents, allowing to obtain the risk assessment of the plant. Thus, the possible shortcomings in the design of systems are identified, providing basis for safety assessment and improving safety. During the environmental licensing, a Quantitative Risk Analysis (QRA), including probabilistic evaluations, is required in order to support the development of the Risk Analysis Study, the Risk Management Program and the Emergency Plan. This article aims to provide an overview of probabilistic risk assessment methodologies and their applications in nuclear and environmental licensing processes of nuclear reactors in Brazil. (author)

  14. E-Licensing in DGFT: A Best E-Governance Application

    Directory of Open Access Journals (Sweden)

    V. S. Rana

    2013-07-01

    Full Text Available This is a case of effective and efficient e-Governance where the licenses are issued electronically by using of IT for web based delivery service in the Directorate General of Foreign Trade (DGFT. Now a days complete licensing procedure is dealing through electronically i.e. Exporter/Importer apply electronically to get the Importer Exporter Code( IEC , submitting the fees as well as necessary documents electronically and received the IEC electronically. Trading Community is also availing the Online facility to submit the application for any licensing scheme, depositing the licensing fees, enclosing the required document from their end. The official procedure is also automated like initiating the note sheet, generating the ecom number, consolation of license fees and issuing the license to the exporter. There is fifty percent role of customs involved in trading. So that Electronic Data Interchange(EDI facility is also established with Customs. In addition to above services, Bank Realization Certificate(BRC is also integrated with this system. Henceforth an Exporter/Importer is equipped with electronic services without visiting to the office of DGFT, Customs and Bank. The web has been strategically leveraged for reengineering and transformation of trade processes for an economic trade facilitation.

  15. SAMA analysis in support of license renewal application for the Fort Calhoun Station

    Energy Technology Data Exchange (ETDEWEB)

    Schneider, R.E.; Jacob, M.C. [Westinghouse Electric Company, Monroeville PA (United States); Hackerott, H.A. [Omaha Public Power District, Omaha NE (United States)

    2004-07-01

    The Omaha Public Power District (OPPD) owns and operates the Fort Calhoun Station Unit 1 (FCS), a single unit Pressurized Water Reactor (PWR) plant. This plant was licensed by the U.S. Nuclear Regulatory Commission (NRC) to operate at a core power level of 1500 MWt for a period of 40 years. In 2002 OPPD submitted a license renewal application to the NRC and recently, this application for a license renewal for a period of 20 more years was approved by the NRC. In order to secure the license renewal, the utility evaluated Severe Accident Mitigation Alternatives (SAMAs) and identified potential risk significant and cost-beneficial plant changes. This process consisted of a review of past industry changes obtained via past SAMA analyses and advanced reactor design efforts as well as a review of FCS PSA (Probabilistic Safety Assessment) insights. While most of the industry-based SAMAs were screened out, the internally focused changes were mostly found to be risk significant and cost effective. This paper presents the SAMA analysis performed in support of the FCS license renewal application and discusses the insights from this analysis. (authors)

  16. 78 FR 32668 - Draft Guidance for Industry: Changes to an Approved Application: Biological Products: Human Blood...

    Science.gov (United States)

    2013-05-31

    ...The Food and Drug Administration (FDA) is announcing the availability of a draft document entitled ``Guidance for Industry: Changes to an Approved Application: Biological Products: Human Blood and Blood Components Intended for Transfusion or for Further Manufacture'' dated June 2013. The draft guidance document provides manufacturers of licensed Whole Blood and blood components intended for......

  17. 77 FR 43374 - Biweekly Notice, Applications and Amendments to Facility Operating Licenses and Combined Licenses...

    Science.gov (United States)

    2012-07-24

    ... of the factors specified in 10 CFR 2.309(c)(1)(i)-(viii). For further details with respect to this...) purification loop interface. This change would only be applicable for the next two fuel cycles. Basis for... an accident that has previously been evaluated? Response: No. The SFP Purification Loop is...

  18. 77 FR 65720 - Biweekly Notice; Applications and Amendments to Facility Operating Licenses and Combined Licenses...

    Science.gov (United States)

    2012-10-30

    ..., see the application for amendment which is available for public inspection at the NRC's PDR, located... of safety is associated with confidence in the ability of the fission product barriers (i.e., fuel... hot channel factor, nuclear enthalpy rise hot channel factor, loss of coolant accident peak...

  19. Structural Biology: Practical NMR Applications

    CERN Document Server

    Teng, Quincy

    2005-01-01

    This textbook begins with an overview of NMR development and applications in biological systems. It describes recent developments in instrument hardware and methodology. Chapters highlight the scope and limitation of NMR methods. While detailed math and quantum mechanics dealing with NMR theory have been addressed in several well-known NMR volumes, chapter two of this volume illustrates the fundamental principles and concepts of NMR spectroscopy in a more descriptive manner. Topics such as instrument setup, data acquisition, and data processing using a variety of offline software are discussed. Chapters further discuss several routine stategies for preparing samples, especially for macromolecules and complexes. The target market for such a volume includes researchers in the field of biochemistry, chemistry, structural biology and biophysics.

  20. 77 FR 1924 - FFP Missouri 15, LLC; FFP Missouri 16, LLC; Notice of Intent To File License Application, Filing...

    Science.gov (United States)

    2012-01-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission FFP Missouri 15, LLC; FFP Missouri 16, LLC; Notice of Intent To File License Application, Filing of Pre-Application Document, and Approving Use of the Traditional Licensing Process...

  1. 77 FR 63812 - FFP Missouri 5, LLC; FFP Missouri 6, LLC; Notice of Intent To File License Application, Filing of...

    Science.gov (United States)

    2012-10-17

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission FFP Missouri 5, LLC; FFP Missouri 6, LLC; Notice of Intent To File License Application, Filing of Pre-Application Document, and Approving Use of the Traditional Licensing Process...

  2. 77 FR 20622 - N.E.W. Hydro LLC; Notice of Application for Amendment of License and Soliciting Comments, Motions...

    Science.gov (United States)

    2012-04-05

    ... Energy Regulatory Commission N.E.W. Hydro LLC; Notice of Application for Amendment of License and...: Amendment of License. b. Project No.: 2744-041 & 042. c. Date Filed: March 14, 2012. d. Applicant: N.E.W... 167, 116 N. State Street, Neshkoro, WI 54869-0167, (920) 293-4628, Ext. 14. i. FERC Contact: John...

  3. 77 FR 75676 - Standard Review Plan for Review of Fuel Cycle Facility License Applications

    Science.gov (United States)

    2012-12-21

    ... Regulatory Commission. ACTION: NUREG revision; extension of comment period. SUMMARY: On December 7, 2012 (77... to NUREG- 1520, Revision 1, ``Standard Review Plan (SRP) for the Review of a License Application for... document is available in ADAMS) is provided the first time that a document is referenced....

  4. 77 FR 14445 - Application for a License To Export Steel Forging

    Science.gov (United States)

    2012-03-09

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Application for a License To Export Steel Forging Pursuant to 10 CFR 110.70(b) ``Public Notice of... Spain. December 15, 2011 head steel head steel February 7, 2012 forging. forging will be XR175...

  5. 77 FR 1956 - Application for a License To Export High-Enriched Uranium

    Science.gov (United States)

    2012-01-12

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Application for a License To Export High-Enriched Uranium Pursuant to 10 CFR 110.70(b) ``Public... Netherlands. Security Complex. Uranium uranium (9.3 targets at December 21, 2011 (93.35%). kilograms U-...

  6. 77 FR 51579 - Application for a License To Export High-Enriched Uranium

    Science.gov (United States)

    2012-08-24

    ... COMMISSION Application for a License To Export High-Enriched Uranium Pursuant to 10 CFR 110.70 (b) ``Public... kilograms For the export of Canada. Complex, July 30, 2012, August Uranium (93.35%). uranium-235 high-enriched 1, 2012, XSNM3726, 11006037. contained in 7.5 uranium in the kilograms uranium. form of...

  7. 78 FR 33448 - Application for a License To Export High-Enriched Uranium

    Science.gov (United States)

    2013-06-04

    ... COMMISSION Application for a License To Export High-Enriched Uranium Pursuant to 10 CFR 110.70 (b) ``Public... targets Canada. Security Complex, May 13, Uranium (93.35%). uranium-235 at the National 2013, May 21, 2013, XSNM3745, contained in 7.5 Research Universal 11006098. kilograms reactor in Canada for uranium....

  8. 77 FR 73056 - Application for a License To Export High-Enriched Uranium

    Science.gov (United States)

    2012-12-07

    ... COMMISSION Application for a License To Export High-Enriched Uranium Pursuant to 10 CFR 110.70 (b) ``Public... targets Belgium. Security Complex. Uranium (93.2%). uranium-235 at CERCA AREVA Romans October 10, 2012 contained in 6.2 in France and to October 12, 2012 kilograms irradiate targets at XSNM3729 uranium. the...

  9. 75 FR 6223 - Application For a License To Export High-Enriched Uranium

    Science.gov (United States)

    2010-02-08

    ... From the Federal Register Online via the Government Publishing Office ] NUCLEAR REGULATORY COMMISSION Application For a License To Export High-Enriched Uranium Pursuant to 10 CFR 110.70(c) ``Public... fabricate Canada. Complex December 21, 2009, Uranium (93.35%). uranium (16.3 targets for December 28,...

  10. 75 FR 7525 - Application for a License To Export High-Enriched Uranium

    Science.gov (United States)

    2010-02-19

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Application for a License To Export High-Enriched Uranium Pursuant to 10 CFR 110.70(c) ``Public... fuel France; Belgium. Security Complex, February 2, Uranium (93.35%). uranium (87.3 elements in...

  11. 76 FR 59173 - Standard Format and Content of License Applications for Conventional Uranium Mills

    Science.gov (United States)

    2011-09-23

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Standard Format and Content of License Applications for Conventional Uranium Mills AGENCY: Nuclear... Conventional Uranium Mills.'' DG- 3024 was a proposed Revision 2 of Regulatory Guide (RG) 3.5. However,...

  12. 75 FR 15743 - Application for a License To Export High-Enriched Uranium

    Science.gov (United States)

    2010-03-30

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Application for a License To Export High-Enriched Uranium Pursuant to 10 CFR 110.70(c) ``Public... fabricate fuel France. Complex, March 3, 2010. Uranium (93.35%). uranium (149.36 elements in March 9,...

  13. 77 FR 73055 - Application for a License To Export High-Enriched Uranium

    Science.gov (United States)

    2012-12-07

    ... COMMISSION Application for a License To Export High-Enriched Uranium Pursuant to 10 CFR 110.70 (b) ``Public... kilograms To fabricate targets The Netherlands. Security Complex. Uranium uranium-235 at CERCA AREVA October... XSNM3730 uranium. targets at the HFR 11006054 Research Reactor in the Netherlands, the BR-2 Reactor...

  14. 76 FR 52994 - Application for a License To Export Heavy Water

    Science.gov (United States)

    2011-08-24

    ... COMMISSION Application for a License To Export Heavy Water Pursuant to 10 CFR 110.70 (b) ``Public Notice of... end-use for China. (D2O--heavy (liters). producing an active water). pharmaceutical ingredient known as CTP-499, which incorporates heavy water as the source of deuterium to achieve the...

  15. 78 FR 25486 - Luminant Generation Company, LLC., Combined License Application for Comanche Peak Nuclear Power...

    Science.gov (United States)

    2013-05-01

    ... COMMISSION Luminant Generation Company, LLC., Combined License Application for Comanche Peak Nuclear Power... Generation Company, LLC. (Luminant) for the proposed facility to be located in Somervell County, Texas. In... or who encounter problems in accessing the documents located in ADAMS should contact the NRC...

  16. 77 FR 39521 - Application for a License To Export Nuclear Reactor Major Components and Equipment

    Science.gov (United States)

    2012-07-03

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Application for a License To Export Nuclear Reactor Major Components and Equipment Pursuant to 10... Reactor internals, Components and For use in Braka nuclear power Company LLC reactor coolant equipment...

  17. 78 FR 37324 - Preparation of Environmental Reports for Nuclear Power Plant License Renewal Applications

    Science.gov (United States)

    2013-06-20

    ... COMMISSION 10 CFR Parts 51 and 54 RIN 3150-AI42 Preparation of Environmental Reports for Nuclear Power Plant..., Supplement 1 (RG 4.2S1), ``Preparation of Environmental Reports for Nuclear Power Plant License Renewal... reports that are submitted with the application for the renewal of a nuclear power plant operating...

  18. 78 FR 19520 - Notice of Invitation to Participate In Coal Exploration License Application UTU-89492, UT

    Science.gov (United States)

    2013-04-01

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE INTERIOR Bureau of Land Management Notice of Invitation to Participate In Coal Exploration License Application UTU-89492, UT AGENCY: Bureau of Land Management, Interior. ACTION: Notice. SUMMARY: All...

  19. 78 FR 7803 - Agency Information Collection Activities: Application-Permit-Special License Unlading-Lading...

    Science.gov (United States)

    2013-02-04

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Application-Permit- Special License Unlading-Lading-Overtime Services AGENCY: U.S. Customs and Border Protection, Department... information collection: 1651-0005. SUMMARY: U.S. Customs and Border Protection (CBP) of the Department...

  20. 75 FR 63476 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2010-10-15

    .... Application Type: QI Change. David A. Knott dba DAK Logistics Services (NVO), 1010 Bluejay Drive, Suisun City, CA 94585. Officer: David A. Knott, Owner (Qualifying Individual), Application Type: New NVO...

  1. 75 FR 34139 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-06-16

    ..., President (Qualifying Individual), Keith Milliner, Vice President, Application Type: Add NVO Service. Aztec...., Albuquerque, NM 87107, Officers: Terry L. Strohl, Operations Manager (Qualifying Individual), William W. Moore... Rosendorf, President (Qualifying Individual), Keith Milliner, Vice President, Application Type: Add...

  2. 76 FR 7847 - Glenn A. Baxter, Application To Renew License for Amateur Radio Service Station K1MAN

    Science.gov (United States)

    2011-02-11

    ... From the Federal Register Online via the Government Publishing Office FEDERAL COMMUNICATIONS COMMISSION Glenn A. Baxter, Application To Renew License for Amateur Radio Service Station K1MAN AGENCY... renew the license for Amateur Radio Service Station K1MAN filed by Glenn A. Baxter should be...

  3. 47 CFR 25.137 - Application requirements for earth stations operating with non-U.S. licensed space stations.

    Science.gov (United States)

    2010-10-01

    ... operating with non-U.S. licensed space stations. 25.137 Section 25.137 Telecommunication FEDERAL... space stations. (a) Earth station applicants or entities filing a “letter of intent” or “Petition for Declaratory Ruling” requesting authority to operate with a non-U.S. licensed space station to serve the...

  4. 77 FR 74186 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2012-12-13

    ... Terrace, Medley, FL 33166, Officers: Hugo E. Martinez, Secretary (QI), Olga P. Martinez, President...: Victor Pezzelato, Vice President Sales (QI), Orit Horn, Managing Member, Application Type: QI...

  5. 77 FR 28880 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2012-05-16

    ..., Inglewood, CA 90301, Officers: Edison Chen, Manager (Qualifying Individual), Wei-Nung (Janus) Lin, Member... A. Case, Vice President (Qualifying Individual), Thomas C. Case, President, Application Type:...

  6. 76 FR 67188 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2011-10-31

    ..., Doral, FL ] 33126, Officers: Ramiro M. Ramirez, Jr., Vice President/Director, (Qualifying Individual), Mirna Ramirez, President/Secretary/Treasurer/ Director, Application Type: Add OFF Service....

  7. 77 FR 12583 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2012-03-01

    ... (NVO), 701 Penhorn Avenue, Unit 1, Secaucus, NJ 07094, Officers: Luciana Line, Secretary (Qualifying...: Luciana Lina, Secretary (Qualifying Individual), Francisco J. Eguiguren, President, Application...

  8. Licensing an assured isolation facility for low-level radioactive waste. Volume 2: Recommendations on the content and review of an application

    Energy Technology Data Exchange (ETDEWEB)

    Silverman, D.J.; Bauser, M.A. [Morgan, Lewis and Bockius, Washington, DC (United States); Baird, R.D. [Rogers and Associates Engineering Corp., Salt Lake City, UT (United States)

    1998-07-01

    This report provides a detailed set of proposed criteria and guidance for the preparation of a license application for an assured isolation facility (AIF). The report is intended to provide a detailed planning basis upon which a prospective applicant may begin pre-licensing discussions with the Nuclear Regulatory Commission and initiate development of a license application. The report may also be useful to the NRC or to state regulatory agencies that may be asked to review such an application. Volume 1 of this report provides background information, and describes the licensing approach and methodology. Volume 2 identifies specific information that is recommended for inclusion in a license application.

  9. 77 FR 72863 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2012-12-06

    ... Francoulon, President. Application Type: Add NVO Service. Kingz International Logistics Inc (OFF), 415 S... telephone at (202) 523-5843 or by email at OTI@fmc.gov . Air 7 Seas Transport Logistics, Inc. (NVO & OFF... & CFO. Application Type: QI Change. All International Solutions Inc. (NVO), 281 E. Redondo Beach...

  10. 75 FR 8720 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-02-25

    ...-Operating Common Carrier--Ocean Transportation Intermediary Applicants: Eastern Logistics LLC, 6 Elna Ct...-Vessel-Operating Common Carrier--Ocean Transportation Intermediary Applicants (Cont'd): HP International LLC dba A&M International, 367 Brooks Street, Elgin, IL 60120. Officers: Marilou Pedress,...

  11. 77 FR 23719 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2012-04-20

    ... Hiebendahl, Secretary. Application Type: Name Change/QI Change. Gandhi International Shipping, Inc. (NVO & OFF), 2358 W. Devon Avenue, Chicago, IL 60659. Officers: Mohammed A. Gandhi, President (Qualifying Individual), Azim Gandhi, Secretary. Application Type: Add NVO Serice. King Solutions, Inc. (NVO &...

  12. 78 FR 35634 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-06-13

    ...: Stan Sing Lau, Vice President (QI), David Koch, CEO. Application Type: QI Change. Logistics Management..., President. Application Type: QI Change. Daisy Mae Concepcion V. Taleon dba DMT Global Logistics (NVO & OFF... Value International dba JP Global Logistics (NVO & OFF), 377 Oyster Point Blvd., Suite 18, South...

  13. 75 FR 66099 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-10-27

    ... Castleton Street, Suite 398, City of Industry, CA 91748, Officers: Jennifer X. McCormick, President (Qualifying Individual), Mark R. McCormick, Secretary/ Treasurer, Application Type: QI Change. British.... Fulton, Assistant Secretary (Qualifying Individual), John E. Ferguson, President, Application Type:...

  14. 78 FR 59923 - Cave Run Energy, LLC; Notice of Intent To File License Application, Filing of Pre-Application...

    Science.gov (United States)

    2013-09-30

    ... Energy Regulatory Commission Cave Run Energy, LLC; Notice of Intent To File License Application, Filing.... Project No.: 14376-001. c. Date Filed: July 21, 2013. d. Submitted By: Cave Run Energy, LLC. e. Name of Project: Cave Run Hydroelectric Project. f. Location: On the Licking River, in Rowan and Bath...

  15. 77 FR 10491 - City of Aspen; Notice of Intent To File License Application, Filing of Pre-Application Document...

    Science.gov (United States)

    2012-02-22

    ... Energy Regulatory Commission City of Aspen; Notice of Intent To File License Application, Filing of Pre....: 13254-002. c. Date Filed: December 12, 2011. d. Submitted By: City of Aspen. e. Name of Project: Castle Creek Hydroelectric Project. f. Location: On Maroon and Castle Creeks near the City of Aspen, in...

  16. 75 FR 53696 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-09-01

    ... (Qualifying Individual), Torri Hideyuki, President. Application Type: Add NVO Service. Norma's Cargo Solutions, LLC (NVO & OFF), 5665 SW 8th Street, Miami, FL 33134. Officer: Norma A. Pineiro, Managing...

  17. 76 FR 67730 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2011-11-02

    ...: Add NVO Service G Max Distributors Inc. (NVO), 6979 NW 84 Avenue Miami, FL 33166, Officers: Hugo D. Carmona, Secretary (Qualifying Individual), Victor Lopez, President, Application Type: New NVO &...

  18. 75 FR 51074 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-08-18

    ..., President, (Qualifying Individual), Application Type: Add Trade Name. CLA Investment & Development, Inc. dba CLA Shipping, Inc., (NVO & OFF), 129 S. 8th Avenue, C, La Puente, CA 91746. Officers: Hong...

  19. 76 FR 70145 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2011-11-10

    ... & OFF), 6701 NW. 7th Street, 135, Miami, FL 33126. Officers: Maria E. Arias, Vice President (Qualifying..., Secretary (Qualifying Individual), Jorge Zambrano, President/Treasurer. Application Type: New NVO &...

  20. 76 FR 4696 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2011-01-26

    ... (NVO & OFF), 2229 NW. 79 Avenue, Doral, FL 33122. Officers: Paul F. Mendoza, COO (Qualifying Individual), Marcela Mendoza, President. Application Type: QI Change. SAPIA Logistics, Inc. (NVO & OFF), 1331...

  1. 75 FR 72824 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-11-26

    ... Logistics Group L.L.C. (NVO & OFF), 2229 NW 79th Avenue, Doral, FL 33122, Officers: Paul Mendoza, COO (Qualifying Individual), Marcela Mendoza, President, Application Type: QI Change. NEC Logistics, Ltd. (NVO),...

  2. 78 FR 1863 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-01-09

    ... telephone at (202) 523-5843 or by email at OTI@fmc.gov . Best Global Logistics USA, Inc. dba Siam Intercargo..., Vice President (QI), Wing-Ham Chu, Director, Application Type: Delete Trade Name Siam...

  3. 78 FR 10173 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-02-13

    ... Okolo and David Newton dba Emunah Global (NVO & OFF), 1904 Farnam Street, Suite 610, Omaha, NE 68102, Officers: David D. Newton, Partner (QI), Jerome Okolo, Partner, Application Type: New NVO & OFF...

  4. 78 FR 75345 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-12-11

    .... Application Type: QI Change. Cromarti Logistics LLC (NVO), 2810 Grants Lake Blvd., Suite 904, Sugar Land, TX... Change. GPL Logistics, Inc (NVO), 18725 E. Gale Avenue, Suite 250, City of Industry, CA 91748....

  5. 78 FR 68444 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-11-14

    .... (NVO & OFF), 2801 NW 7th Avenue, Suite 107, Miami, FL 33122, Officers: Ricardo Tovar, President (QI), Miguel Tovar, Vice President, Application Type: QI Change. BJJ Enterprise LLC (OFF), 121 North...

  6. 75 FR 20997 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-04-22

    ... Individual). Won S. Jang, President/CEO/Secretary, Application Type: QI Change. Intertrade Systems Inc. (OFF... Name Change. SCM Solutions Corp. (OFF), 32938 Tamina Road, Suite 100, Magnolia, TN 77354....

  7. 75 FR 48686 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2010-08-11

    ... Systems, Inc. dba MILS dba Fesco Integrated Transport (NVO & OFF), 1000 Second Avenue, Suite 1310, Seattle... (Qualifying Individual), Kenryo Senda, President/CEO, Application Type: QI Change. Praxis SCM, LLC (NVO &...

  8. Thermophilic molds: Biology and applications.

    Science.gov (United States)

    Singh, Bijender; Poças-Fonseca, Marcio J; Johri, B N; Satyanarayana, Tulasi

    2016-11-01

    Thermophilic molds thrive in a variety of natural habitats including soils, composts, wood chip piles, nesting materials of birds and other animals, municipal refuse and others, and ubiquitous in their distribution. These molds grow in simple media containing carbon and nitrogen sources and mineral salts. Polyamines are synthesized in these molds and the composition of lipids varies considerably, predominantly containing palmitic, oleic and linoleic acids with low levels of lauric, palmiotoleic and stearic acids. Thermophilic molds are capable of efficiently degrading organic materials by secreting thermostable enzymes, which are useful in the bioremediation of industrial wastes and effluents that are rich in oil, heavy metals, anti-nutritional factors such as phytic acid and polysaccharides. Thermophilic molds synthesize several antimicrobial substances and biotechnologically useful miscellaneous enzymes. The analysis of genomes of thermophilic molds reveals high G:C contents, shorter introns and intergenic regions with lesser repetitive sequences, and further confirms their ability to degrade agro-residues efficiently. Genetic engineering has aided in ameliorating the characteristics of the enzymes of thermophilic molds. This review is aimed at focusing on the biology of thermophilic molds with emphasis on recent developments in the analysis of genomes, genetic engineering and potential applications.

  9. 77 FR 73060 - Standard Review Plan for Review of Fuel Cycle Facility License Applications

    Science.gov (United States)

    2012-12-07

    ...The U.S. Nuclear Regulatory Commission (NRC) is planning to revise NUREG-1520, Revision 1, ``Standard Review Plan (SRP) for the Review of a License Application for a Fuel Cycle Facility.'' The staff proposes to revise NUREG-1520 to provide guidance and further clarify several technical areas. In addition to revising NUREG-1520, the staff is also planning to issue Interim Staff Guidance (ISG)......

  10. 76 FR 41258 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2011-07-13

    .... LTA Import & Export, Inc. (NVO & OFF), 14331 SW 120th Street, 203, Miami, FL 33186. Officers: Eric E..., Managing Member (Qualifying Individual), Bradley D. Louden, Managing Member. Application Type: Name Change...: Rodney A. Gonzalez, Secretary (Qualifying Individual), James W. Coley, Managing...

  11. 76 FR 59128 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2011-09-23

    ... at OTI@fmc.gov . APLUS Worldwide Logistics, Corp. (NVO & OFF), 2129 NW., 79th Avenue, Doral, FL 33122... Individual) Akira Tsuneda, Director/COB. Application Type: QI Change. Contour Logistics Inc. (NVO & OFF... Energy & Marine Services LLC (NVO & OFF), 16607 Central Green Blvd., 200, Houston, TX 77032....

  12. 77 FR 50688 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2012-08-22

    ..., President. Application Type: QI Change. Transera International Logistics (OFF), 10343 Sam Houston Park Drive... telephone at (202) 523-5843 or by email at OTI@fmc.gov . Anselm K. Nwankwo dba Anze Global Logistics (NVO... Logistics Inc. (NVO & OFF), 288 West 238th Street 5h, Bronx, NY 10463. Officer: Angel N. Espinoza,...

  13. 76 FR 75566 - Notice of Availability of Combined License Applications

    Science.gov (United States)

    2011-12-02

    ... Carolinas, Inc., and Florida Power & Light Company. ADDRESSES: You can access publicly available documents... into ADAMS, which provides text and image files of the NRC's public documents. If you do not have..., Florida Power & Light Company submitted an application for COLs for two AP1000 advanced...

  14. 77 FR 20398 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2012-04-04

    ... Type: QI Change. Alternative Global Logistic, LLC (OFF), 8551 NW. 12th Street, Pembroke Pines, FL 33024..., Memphis, TN 38118, Officers: Patrick W. DePriest, Vice President Export Operations, (Qualifying Individual...), David G. Pirtskhalava, Vice President, Application Type: Business Structure Change. Pinki...

  15. Biological Applications of Synthetic Nanomachines

    Science.gov (United States)

    Kagan, Daniel Robert

    The field of synthetic nano/microscale propulsion devices has been rapidly expanding because of their ability to possess many key features necessary for bioanalytical applications on biological microchip devices and targeted in vivo delivery. Past studies focused on developing powerful and easily controllable motors by investigating different propulsion schemes (e.g. electrophoretic, bubble release, magnetically propelled) for use in physiological environments. These engineering advancements and the nanomotors inherit capabilities have allowed for their use in three research areas: motion-based biosensing, cellular and biomolecular isolation, and targeted drug delivery. The first research area investigates a unique speed increase of electrophoretically propelled nanomotors when in the presence of silver ions. Au/Pt nanomotors propel by the electrocatalytic decomposition of H2O2 fuel. While most metal ions resulted in a decrease in speed to near Brownian levels, Ag+ has shown a steady increase in speed from 10microm/s to 52microm/s over the micro-molar range. This phenomenon was exploited by tagging nucleic acid detector probes with Ag nanoparticles when conducting simple sandwich assays. This resulted in a cheap, fast, and sensitive, motion-based readout of the concentration-dependent DNA target present on the sandwich assay. The second area of research involved the bioisolation of nucleic acids, protein, bacteria, and cancer cells by bubble-based microrockets. These microrockets contain a platinum interior to catalyze peroxide fuel and can be easily functionalized with antibodies and nucleic acid capture probes to isolate target biomolecules. The motion of these micro-isolation devices creates convection for faster isolation and can be used to transport the biomolecules to a clean environment. The third area of research is focused on targeted drug delivery by various propulsion methods. The ability of nanomotors to transport PLGA and liposome drug vesicles to

  16. Application of phase stretch transform to plate license identification under blur and noise conditions (Conference Presentation)

    Science.gov (United States)

    Asghari, Hossein; Hadar, Ofer; Jalali, Bahram

    2016-09-01

    This paper deals with implementing a new algorithm for edge detection based on the Phase Stretch Transform (PST) for purposes of car plate license recognition. In PST edge detection algorithm, the image is first filtered with a spatial kernel followed by application of a nonlinear frequency-dependent phase. The output of the transform is the phase in the spatial domain. The main step is the 2-D phase function which is typically applied in the frequency domain. The amount of phase applied to the image is frequency dependent with higher amount of phase applied to higher frequency features of the image. Since sharp transitions, such as edges and corners, contain higher frequencies, PST emphasizes the edge information. Features can be further enhanced by applying thresholding and morphological operations. Here we investigate the influence of noise and blur on the ability to recognize the characters in the plate license, by comparison of our suggested algorithm with the well known Canny algorithm. We use several types of noise distributions among them, Gaussian noise, salt and paper noise and uniform distributed noise, with several levels of noise variances. The simulated blur is related to the car velocity and we applied several filters representing different velocities of the car. Another interesting degradation that we intend to investigate is the cases that Laser shield license plate cover is used to distort the image taken by the authorities. Our comparison results are presented in terms of True positive, False positive and False negative probabilities.

  17. 47 CFR 1.945 - License grants.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false License grants. 1.945 Section 1.945... Applications and Proceedings Application Requirements and Procedures § 1.945 License grants. (a) License grants—auctionable license applications. Procedures for grant of licenses that are subject to competitive...

  18. 75 FR 74700 - Grand River Dam Authority; Notice of Application for Amendment of License and Soliciting Comments...

    Science.gov (United States)

    2010-12-01

    ... Energy Regulatory Commission Grand River Dam Authority; Notice of Application for Amendment of License.... Date Filed: October 26, 2010. d. Applicant: Grand River Dam Authority. e. Name of Project: Markham.... 791(a)-825(r). h. Applicant Contact: Tamara E. Jahnke, Grand River Dam Authority, P.O. Box 409,...

  19. 75 FR 16090 - Grand River Dam Authority; Notice of Application for Amendment of License and Soliciting Comments...

    Science.gov (United States)

    2010-03-31

    ... Energy Regulatory Commission Grand River Dam Authority; Notice of Application for Amendment of License.... Date Filed: March 11, 2010, supplemented on March 17, 2010. d. Applicant: Grand River Dam Authority. e.... 791a-825r. h. Applicant Contact: Ms. Tamara E. Jahnke, Assistant General Council, Grand Dam...

  20. 78 FR 75376 - Entergy Operations, Inc.; Combined License Application for River Bend Unit 3, Exemption From the...

    Science.gov (United States)

    2013-12-11

    ... Combined License (COL) application. The NRC staff reviewed this request and determined that it is... coincident with, the resumption of the COL application review or by December 31, 2014, whichever comes first... Background On September 25, 2008, EOI submitted to the NRC a COL application for one Economic...

  1. 78 FR 75379 - Entergy Operations, Inc.; Combined License Application for Grand Gulf Unit 3; Exemption From the...

    Science.gov (United States)

    2013-12-11

    ... Combined License (COL) application. The NRC staff reviewed this request and determined that it is... coincident with, the resumption of the COL application review or by December 31, 2014, whichever comes first... Background On February 27, 2008, EOI submitted to the NRC a COL application for one Economic...

  2. 75 FR 6018 - Boyce Hydro Power, LLC; Notice of Application for Amendment of License and Soliciting Comments...

    Science.gov (United States)

    2010-02-05

    ... Energy Regulatory Commission Boyce Hydro Power, LLC; Notice of Application for Amendment of License and..., and supplemented on November 4, 2009. d. Applicant: Boyce Hydro Power, LLC (BHP). e. Name of Project.... Applicant Contact: Frank Christie, General Manager, Boyce Hydro Power, LLC, 6000 South M-30, P.O. Box...

  3. Synthetic biology: programming cells for biomedical applications.

    Science.gov (United States)

    Hörner, Maximilian; Reischmann, Nadine; Weber, Wilfried

    2012-01-01

    The emerging field of synthetic biology is a novel biological discipline at the interface between traditional biology, chemistry, and engineering sciences. Synthetic biology aims at the rational design of complex synthetic biological devices and systems with desired properties by combining compatible, modular biological parts in a systematic manner. While the first engineered systems were mainly proof-of-principle studies to demonstrate the power of the modular engineering approach of synthetic biology, subsequent systems focus on applications in the health, environmental, and energy sectors. This review describes recent approaches for biomedical applications that were developed along the synthetic biology design hierarchy, at the level of individual parts, of devices, and of complex multicellular systems. It describes how synthetic biological parts can be used for the synthesis of drug-delivery tools, how synthetic biological devices can facilitate the discovery of novel drugs, and how multicellular synthetic ecosystems can give insight into population dynamics of parasites and hosts. These examples demonstrate how this new discipline could contribute to novel solutions in the biopharmaceutical industry.

  4. Model tracking system for low-level radioactive waste disposal facilities: License application interrogatories and responses

    Energy Technology Data Exchange (ETDEWEB)

    Benbennick, M.E.; Broton, M.S.; Fuoto, J.S.; Novgrod, R.L.

    1994-08-01

    This report describes a model tracking system for a low-level radioactive waste (LLW) disposal facility license application. In particular, the model tracks interrogatories (questions, requests for information, comments) and responses. A set of requirements and desired features for the model tracking system was developed, including required structure and computer screens. Nine tracking systems were then reviewed against the model system requirements and only two were found to meet all requirements. Using Kepner-Tregoe decision analysis, a model tracking system was selected.

  5. Licensing the ACR-700 in the USA; progress on the pre-application review

    Energy Technology Data Exchange (ETDEWEB)

    Ion, R. [Atomic Energy of Canada Ltd., Mississauga, Ontario (Canada); Langman, V. [AECL Technologies, Gaithersburg, Maryland (United States); Snell, V. [Atomic Energy of Canada Ltd., Mississauga, Ontario (Canada); Reid, C. [Bechtel Power Corp., San Francisco, California (United States)

    2004-07-01

    The ACR-700 is an evolutionary reactor design, which incorporates the inherent safety features of the CANDU products, as well as the successful operating experience of the current CANDU 6 reactors. The improvements to the ACR-700 from CANDU 6 result in significant reductions in capital and operating costs as well as enhanced safety. AECL Technologies (AECLT, a wholly owned US subsidiary of Atomic Energy of Canadian Limited) is the proponent for a pre-application review of the ACR-700 with the US Nuclear Regulatory Commission (NRC) in the United States. This pre-application review will be completed shortly and will support an application to the US NRC for Standard Design Certification (SDC). AECL Technologies' overall objective for the pre-application review of the ACR-700 is to obtain an understanding, from interactions with the USNRC, of the scope, cost, and the schedule to obtain a Standard Design Certification for the ACR-700. The pre-application review will address licensing issues associated with the CANDU reactor technologies in ACR-700 that depart from the light water reactor, pressure-vessel based regulatory framework in the USA. Therefore, during the course of the pre-application review, any major US NRC issues with the ACR-700 design will be identified early and the scope of the work required to address these concerns, along with associated completion schedules, will be formulated and ultimately agreed upon with the US NRC. AECLT has been informed by the NRC staff that the results of their pre-application review will be documented in a Safety Assessment Report (SAR), which will state whether there are any major impediments to licensing the ACR in the United States. In particular, the SAR should provide confirmation of the licensing criteria applicable to the ACR, provide an assessment of the completeness of AECL 's Research and Development (R and D) programs that exist or are planned in support of the ACR, provide an assessment of the

  6. TOPICAL REVIEW: Biological applications of colloidal nanocrystals

    Science.gov (United States)

    Parak, Wolfgang J.; Gerion, Daniele; Pellegrino, Teresa; Zanchet, Daniela; Micheel, Christine; Williams, Shara C.; Boudreau, Rosanne; LeGros, Mark A.; Larabell, Carolyn A.; Alivisatos, A. Paul

    2003-07-01

    Due to their interesting properties, research on colloidal nanocrystals has moved in the last few years from fundamental research to first applications in materials science and life sciences. In this review some recent biological applications of colloidal nanocrystals are discussed, without going into biological or chemical details. First, the properties of colloidal nanocrystals and how they can be synthesized are described. Second, the conjugation of nanocrystals with biological molecules is discussed. And third, three different biological applications are introduced: (i) the arrangement of nanocrystal-oligonucleotide conjugates using molecular scaffolds such as single-stranded DNA, (ii) the use of nanocrystal-protein conjugates as fluorescent probes for cellular imaging, and (iii) a motility assay based on the uptake of nanocrystals by living cells.

  7. 47 CFR 5.71 - License period.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false License period. 5.71 Section 5.71...) Applications and Licenses § 5.71 License period. (a) The regular license period for stations in the Experimental Radio Service is either 2 or 5 years. An applicant desiring to apply for a 5-year license...

  8. Fluorescent Sensors for Biological Applications

    Directory of Open Access Journals (Sweden)

    Hui-wang Ai

    2014-09-01

    Full Text Available Fluorescence is one of the most important analytical methods used in biological studies. In the past decade or two, instrumentation in this field has greatly advanced, and now it is possible to detect single photons or fluorescent molecules [1,2], or break the Abbe diffraction limit to distinguish two points spaced less than 50 nm apart [3]. Concurrently, the development of improved fluorescent probes, which can be coupled with state-of-the-art instruments, has been equally important. This special issue on “fluorescent biosensors” in Sensors reports recent results from eight research groups in the field of sensor development. It includes three review articles, and six research articles reporting original results. [...

  9. Database Transformations for Biological Applications

    Energy Technology Data Exchange (ETDEWEB)

    Overton, C.; Davidson, S. B.; Buneman, P.; Tannen, V.

    2001-04-11

    The goal of this project was to develop tools to facilitate data transformations between heterogeneous data sources found throughout biomedical applications. Such transformations are necessary when sharing data between different groups working on related problems as well as when querying data spread over different databases, files and software analysis packages.

  10. 77 FR 30518 - Grand River Dam Authority; Notice of Application for Amendment of License and Soliciting Comments...

    Science.gov (United States)

    2012-05-23

    ... Energy Regulatory Commission Grand River Dam Authority; Notice of Application for Amendment of License... 11, 2012. d. Applicant: Grand River Dam Authority. e. Name of Project: Pensacola Hydroelectric... Counsel, Grand River Dam Authority, P.O. Box 409, Vinita, Oklahoma 74301, (918) 256-5545. i. FERC...

  11. 75 FR 4363 - Grand River Dam Authority; Notice of Application for Amendment of License and Soliciting Comments...

    Science.gov (United States)

    2010-01-27

    ... Energy Regulatory Commission Grand River Dam Authority; Notice of Application for Amendment of License..., 2009. d. Applicant: Grand River Dam Authority. e. Name of Project: Markham Ferry Hydroelectric Project... Contact: D. Casey Davis, Grand River Dam Authority, P.O. Box 409, 226 West Dwain Willis Avenue,...

  12. 75 FR 61458 - Grand River Dam Authority; Notice of Application for Amendment of License and Soliciting Comments...

    Science.gov (United States)

    2010-10-05

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Grand River Dam Authority; Notice of Application for Amendment of License.... Date Filed: May 27, 2010, supplemented on August 12 and August 16, 2010. d. Applicant: Grand River...

  13. 75 FR 28595 - City of Idaho Falls; Notice of Application for Amendment of License and Soliciting Comments...

    Science.gov (United States)

    2010-05-21

    ... Energy Regulatory Commission City of Idaho Falls; Notice of Application for Amendment of License and..., 2010, March 31, 2010. d. Applicant: City of Idaho Falls. e. Name of Project: Idaho Falls Hydroelectric Project. f. Location: The proposed non-project use is in Idaho Falls, Idaho. g. Filed Pursuant to:...

  14. 78 FR 4465 - PPL Bell Bend, LLC; Combined License Application for Bell Bend Nuclear Power Plant; Exemption

    Science.gov (United States)

    2013-01-22

    ... COMMISSION PPL Bell Bend, LLC; Combined License Application for Bell Bend Nuclear Power Plant; Exemption 1.0... Approvals for Nuclear Power Plants.'' This reactor is to be identified as Bell Bend Nuclear Power Plant... (RCOL) application for UniStar's Calvert Cliffs Nuclear Power Plant, Unit 3 (CCNPP3). The......

  15. 76 FR 81992 - PPL Bell Bend, LLC; Combined License Application for Bell Bend Nuclear Power Plant; Exemption

    Science.gov (United States)

    2011-12-29

    ... COMMISSION PPL Bell Bend, LLC; Combined License Application for Bell Bend Nuclear Power Plant; Exemption 1.0..., Certifications, and Approvals for Nuclear Power Plants.'' This reactor is to be identified as Bell Bend Nuclear Power Plant (BBNPP), in Salem County, Pennsylvania. The BBNPP COL application incorporates by...

  16. 77 FR 21761 - Alice Falls Corporation, Alice Falls Hydro, LLC; Notice of Application for Transfer of License...

    Science.gov (United States)

    2012-04-11

    ... Energy Regulatory Commission Alice Falls Corporation, Alice Falls Hydro, LLC; Notice of Application for Transfer of License, and Soliciting Comments and Motions To Intervene On February 23, 2012, Alice Falls Corporation (transferor) and Alice Falls Hydro, LLC (transferee) filed an ] application for transfer...

  17. Biological applications of nanocrystalline diamond

    OpenAIRE

    Williams, Oliver; Daenen, Michael; Haenen, Ken

    2007-01-01

    Nanocrystalline diamond films have generated substantial interest in recent years due to their low cost, extreme properties and wide application arena. Diamond is chemically inert, has a wide electrochemical window and is stable in numerous harsh environments. Nanocrystalline diamond has the advantage of being readily grown on a variety of substrates at very low thickness, resulting in smooth conformal coatings with high transparency. These films can be doped from highly insulating to metalli...

  18. 76 FR 79203 - Prospective Grant of Exclusive License: Veterinary Biological Products for Swine Influenza Vaccines

    Science.gov (United States)

    2011-12-21

    ... Biological Products for Swine Influenza Vaccines AGENCY: National Institutes of Health, Public Health Service... methods of use as Veterinary Influenza Vaccines. Sustained outbreaks of highly pathogenic influenza in animals increase the risk of reassortment and adaption to humans. This technology describes DNA...

  19. Quantum metrology and its application in biology

    Science.gov (United States)

    Taylor, Michael A.; Bowen, Warwick P.

    2016-02-01

    Quantum metrology provides a route to overcome practical limits in sensing devices. It holds particular relevance to biology, where sensitivity and resolution constraints restrict applications both in fundamental biophysics and in medicine. Here, we review quantum metrology from this biological context, focusing on optical techniques due to their particular relevance for biological imaging, sensing, and stimulation. Our understanding of quantum mechanics has already enabled important applications in biology, including positron emission tomography (PET) with entangled photons, magnetic resonance imaging (MRI) using nuclear magnetic resonance, and bio-magnetic imaging with superconducting quantum interference devices (SQUIDs). In quantum metrology an even greater range of applications arise from the ability to not just understand, but to engineer, coherence and correlations at the quantum level. In the past few years, quite dramatic progress has been seen in applying these ideas into biological systems. Capabilities that have been demonstrated include enhanced sensitivity and resolution, immunity to imaging artefacts and technical noise, and characterization of the biological response to light at the single-photon level. New quantum measurement techniques offer even greater promise, raising the prospect for improved multi-photon microscopy and magnetic imaging, among many other possible applications. Realization of this potential will require cross-disciplinary input from researchers in both biology and quantum physics. In this review we seek to communicate the developments of quantum metrology in a way that is accessible to biologists and biophysicists, while providing sufficient details to allow the interested reader to obtain a solid understanding of the field. We further seek to introduce quantum physicists to some of the central challenges of optical measurements in biological science. We hope that this will aid in bridging the communication gap that exists

  20. Functionalized Nanodiamonds for Biological and Medical Applications.

    Science.gov (United States)

    Lai, Lin; Barnard, Amanda S

    2015-02-01

    Nanodiamond is a promising material for biological and medical applications, owning to its relatively inexpensive and large-scale synthesis, unique structure, and superior optical properties. However, most biomedical applications, such as drug delivery and bio-imaging, are dependent upon the precise control of the surfaces, and can be significantly affected by the type, distribution and stability of chemical funtionalisations of the nanodiamond surface. In this paper, recent studies on nanodiamonds and their biomedical applications by conjugating with different chemicals are reviewed, while highlighting the critical importance of surface chemical states for various applications.

  1. Application of molecular biology in exercise physiology.

    Science.gov (United States)

    Booth, F W

    1989-01-01

    Past progress in exercise biochemical research has often depended on the use of knowledge and techniques which were originally reported from other disciplines. With the advent of newer methodologies in molecular biology, the purpose of this review has been to document the status of information gained from the application of molecular biological techniques to questions in exercise physiology. Furthermore, this review has speculated how new methods in molecular biology might be employed to answer classic questions in exercise physiology. A powerful revolution in science, that is, molecular biology, will provide new information about exercise mechanisms, which ideally will improve the training programs for elite athletes as well as continue to be associated with the public's interest in exercise training.

  2. Liquid Crystalline Materials for Biological Applications.

    Science.gov (United States)

    Lowe, Aaron M; Abbott, Nicholas L

    2012-03-13

    Liquid crystals have a long history of use as materials that respond to external stimuli (e.g., electrical and optical fields). More recently, a series of investigations have reported the design of liquid crystalline materials that undergo ordering transitions in response to a range of biological interactions, including interactions involving proteins, nucleic acids, viruses, bacteria and mammalian cells. A central challenge underlying the design of liquid crystalline materials for such applications is the tailoring of the interface of the materials so as to couple targeted biological interactions to ordering transitions. This review describes recent progress toward design of interfaces of liquid crystalline materials that are suitable for biological applications. Approaches addressed in this review include the use of lipid assemblies, polymeric membranes containing oligopeptides, cationic surfactant-DNA complexes, peptide-amphiphiles, interfacial protein assemblies and multi-layer polymeric films.

  3. Applications of nanoparticles in biology and medicine

    Directory of Open Access Journals (Sweden)

    Salata OV

    2004-04-01

    Full Text Available Abstract Nanomaterials are at the leading edge of the rapidly developing field of nanotechnology. Their unique size-dependent properties make these materials superior and indispensable in many areas of human activity. This brief review tries to summarise the most recent developments in the field of applied nanomaterials, in particular their application in biology and medicine, and discusses their commercialisation prospects.

  4. 47 CFR 78.29 - License period.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 4 2010-10-01 2010-10-01 false License period. 78.29 Section 78.29... SERVICE Applications and Licenses § 78.29 License period. Licenses for CARS stations will be issued for a period not to exceed five (5) years. On and after February 1, 1966, licenses for CARS stations...

  5. 76 FR 40903 - Grand River Dam Authority; Notice of Application for Amendment of License and Soliciting Comments...

    Science.gov (United States)

    2011-07-12

    ... Energy Regulatory Commission Grand River Dam Authority; Notice of Application for Amendment of License...: Grand River Dam Authority. e. Name of Project: Salina Pumped Storage Project. f. Location: The project... River Dam Authority, P.O. Box 409, Vinita, OK 73401-0409. Tel: (918) 256-5545. i. FERC Contact:...

  6. 76 FR 7831 - Grand River Dam Authority; Notice of Application for Amendment of License and Soliciting Comments...

    Science.gov (United States)

    2011-02-11

    ... Energy Regulatory Commission Grand River Dam Authority; Notice of Application for Amendment of License...: Grand River Dam Authority. e. Name of Project: Markham Ferry Project. f. Location: The project is..., Grand River Dam Authority, P.O. Box 409, Vinita, OK 73401-0409. Tel: (918) 256-5545. i. FERC...

  7. 18 CFR 16.10 - Information to be provided by an applicant for new license: Filing requirements.

    Science.gov (United States)

    2010-04-01

    ... generation lost at the project over the last five years because of unscheduled outages, including the cause... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Information to be provided by an applicant for new license: Filing requirements. 16.10 Section 16.10 Conservation of...

  8. 76 FR 81994 - UniStar Nuclear Energy; Combined License Application for Calvert Cliffs Nuclear Power Plant, Unit...

    Science.gov (United States)

    2011-12-29

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION UniStar Nuclear Energy; Combined License Application for Calvert Cliffs Nuclear Power Plant, Unit 3; Exemption 1.0 Background: UniStar Nuclear Energy (UNE) submitted to the U.S. Nuclear...

  9. 78 FR 4467 - UniStar Nuclear Energy, Combined License Application for Calvert Cliffs Power Plant, Unit 3...

    Science.gov (United States)

    2013-01-22

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION UniStar Nuclear Energy, Combined License Application for Calvert Cliffs Power Plant, Unit 3, Exemption 1.0 Background UniStar Nuclear Energy (UNE), on behalf of Calvert Cliffs Nuclear Project, LLC...

  10. 77 FR 35079 - License Renewal Application for Seabrook Station, Unit 1 ; NextEra Energy Seabrook, LLC

    Science.gov (United States)

    2012-06-12

    .... Address questions about NRC dockets to Carol Gallagher; telephone: 301-492- 3668; email: Carol.Gallagher... INFORMATION CONTACT: Mr. Michael Wentzel, Division of License Renewal, Office of Nuclear Reactor Regulation, U...: michael.wentzel@nrc.gov . SUPPLEMENTARY INFORMATION: Discussion NextEra submitted an application...

  11. 76 FR 11522 - In the Matter of Progress Energy Florida, Inc. (Combined License Application, Levy County Nuclear...

    Science.gov (United States)

    2011-03-02

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION In the Matter of Progress Energy Florida, Inc. (Combined License Application, Levy County Nuclear... relating to pending appeal filed by the Nuclear Regulatory Commission staff in this case. Mr. Dehmel...

  12. 77 FR 36012 - PPL Bell Bend, LLC; Bell Bend Nuclear Power Plant Combined License Application; Notice of Intent...

    Science.gov (United States)

    2012-06-15

    ... COMMISSION PPL Bell Bend, LLC; Bell Bend Nuclear Power Plant Combined License Application; Notice of Intent... its Bell Bend Nuclear Power Plant (BBNPP) site, located west of the existing Susquehanna Steam... by relocating the power block footprint and other plant components. For purposes of developing...

  13. 75 FR 16091 - Glen Falls Hydro, LLC; Essex Energy Partners, LLC; Notice of Application for Transfer of License...

    Science.gov (United States)

    2010-03-31

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Glen Falls Hydro, LLC; Essex Energy Partners, LLC; Notice of Application for Transfer of License, and Soliciting Comments and Motions To Intervene March 24, 2010. On March 8,...

  14. 75 FR 69063 - Port Bailey Wild Enterprises, LLC; PB Energy, Inc.; Notice of Application for Transfer of License...

    Science.gov (United States)

    2010-11-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Port Bailey Wild Enterprises, LLC; PB Energy, Inc.; Notice of Application for Transfer of License, and Soliciting Comments and Motions To Intervene November 3, 2010. On...

  15. Mammalian synthetic biology: emerging medical applications.

    Science.gov (United States)

    Kis, Zoltán; Pereira, Hugo Sant'Ana; Homma, Takayuki; Pedrigi, Ryan M; Krams, Rob

    2015-05-06

    In this review, we discuss new emerging medical applications of the rapidly evolving field of mammalian synthetic biology. We start with simple mammalian synthetic biological components and move towards more complex and therapy-oriented gene circuits. A comprehensive list of ON-OFF switches, categorized into transcriptional, post-transcriptional, translational and post-translational, is presented in the first sections. Subsequently, Boolean logic gates, synthetic mammalian oscillators and toggle switches will be described. Several synthetic gene networks are further reviewed in the medical applications section, including cancer therapy gene circuits, immuno-regulatory networks, among others. The final sections focus on the applicability of synthetic gene networks to drug discovery, drug delivery, receptor-activating gene circuits and mammalian biomanufacturing processes.

  16. 9 CFR 102.6 - Conditional licenses.

    Science.gov (United States)

    2010-01-01

    ... AGRICULTURE VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS LICENSES FOR BIOLOGICAL...) of this part, issue a conditional U.S. Veterinary Biological Product License to an establishment... available, the Administrator shall either reissue the U.S. Veterinary Biological Product License or allow...

  17. Technology licensing by advertising supported media platforms: An application to internet search engines

    OpenAIRE

    Sapi, Geza; Suleymanova, Irina

    2011-01-01

    We develop a duopoly model with advertising supported platforms and analyze incentives of a superior firm to license its advanced technologies to an inferior rival. We highlight the role of two technologies characteristic for media platforms: The technology to produce content and to place advertisements. Licensing incentives are driven solely by indirect network effects arising fromthe aversion of users to advertising. We establish a relationship between licensing incentives and the nature of...

  18. Porphyrin Microparticles for Biological and Biomedical Applications

    Science.gov (United States)

    Huynh, Elizabeth

    Lipids are one of the critical building blocks of life, forming the plasma membrane of cells. In addition, porphyrins also play an equally important role in life, for example, through carrying oxygen in blood. The importance of both these components is evident through the biological and biomedical applications of supramolecular structures generated from lipids and porphyrins. This thesis investigates new porphyrin microparticles based on porphyrin-lipid architecture and their potential applications in biology and medicine. In Chapter 1, a background on lipid and porphyrin-based supramolecular structures is presented and design considerations for generating multifunctional agents. Chapter 2 describes the generation of a monolayer porphyrin microparticle as a dual-modal ultrasound and photoacoustic contrast agent and subsequently, a trimodal ultrasound, photoacoustic and fluorescence contrast agent. Chapter 3 examines the optical and morphological response of these multimodality ultrasound-based contrast agents to low frequency, high duty cycle ultrasound that causes the porphyrin microparticles to convertinto nanoparticles. Chapter 4 examines the generation of bilayer micrometer-sized porphyrin vesicles and their properties. Chapter 5 presents a brief summary and potential future directions. Although these microscale structures are similar in structure, the applications of these structures greatly differ with potential applications in biology and also imaging and therapy of disease. This thesis aims to explore and demonstrate the potential of new simplified, supramolecular structures based on one main building block, porphyrin-lipid.

  19. Applicability of IBAMA normative instruction 146-2007 to pipeline environmental licensing

    Energy Technology Data Exchange (ETDEWEB)

    Basbaum, Marcos A.; Fonseca, Renata A.A. [Servicos de Engenharia Emilio Baumgart Ltda. (SEEBLA), Rio de Janeiro, RJ(Brazil)], e-mail: mbasbaum.seebla@petrobras.com.br, e-mail: renataamorim.seebla@petrobras.com.br; Torggler, Bianca F.; Fernandes, Renato; Guimaraes, Ricardo Z.P. [Petroleo do Brasil S.A. (PETROBRAS), Rio de Janeiro, RJ (Brazil)], e-mail: torggler@petrobras.com.br, e-mail: renatofer@petrobras.com.br, e-mail: rzaluar@petrobras.com.br

    2009-07-01

    PETROBRAS has been conducting several wildlife programs in response to environmental licensing requirements from IBAMA (Brazilian environmental agency). Since 2007, IBAMA has been requiring the application of the Normative Instruction n. 146 (NI), which establishes criteria for wildlife management in the influence areas of new enterprises. However, experience from PETROBRAS has shown that many of the NI articles are not applicable to pipelines. Thus, a critical analysis of this NI was done based on data from company's wildlife programs. Our analysis indicated that the main NI items that do not apply to pipelines are: 1 - Animal rescue programs in deforested areas and selection of release areas, 2 - More than two fauna monitoring campaigns a year, 3 - Monitoring programs involving all groups of terrestrial vertebrates and also invertebrate classes. The non-applicability of these items is due to: 1 - The majority of pipelines are built in areas without significant native vegetation or with small fragments only, which are usually in secondary stage of ecological succession. Most pipelines scarcely involve removal of primary vegetation, 2 - Pipelines have an influence area restricted to a range of 800 m and the construction directly affects only strips from 20 m to 30 m, 3 - The most relevant impacts associated with pipelines are temporary, and do not persist during their operation. (author)

  20. Methodology of licensing for field applications of Weld Inlay and Ultrasonic Nanocrystal Surface Modification

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Hong Seok; Jung, Kwang Woon [KEPCO KPS, Naju (Korea, Republic of); Park, Ik Keun [Seoul National Univ, Seoul (Korea, Republic of); Pyun, Young Sik [Sun Moon University, Asan (Korea, Republic of)

    2015-10-15

    There are many attempts to relieve or change high tensile residual stress to compressive residual stress for a mitigation of PWSCC. LSP(Laser Shot Peening) and WJP(Water Jet Peening) have been applied in several NPPs of Japan and technical and topical reports under Materials Reliability Program of EPRI(Electric Power Research Institute ) USA have been developed. Weld Inlay technology and UNSM(Ultrasonic Nanocrystal Surface Modification) technology using ultrasonic vibration energy as the method for a mitigation of tensile residual stress generated after Weld Inlay have been developing in Korea. So in this study, the methodology of licensing for field application of these technologies is suggested. The results of residual stress measured before and after UNSM for Weld Inlay specimens using UNSM equipment of domestic source technology holdings to obtain an equivalent effect, which has the simplification of equipment and the flexibility of field application in comparison with LSP and WJP, indicated to obtain a mitigation effect of residual stress until 0.5 mm of surface depth. In order to accomplish field application of Weld Inlay that the Code Case was already developed, the approval of a regulation agency through the preparation of TR or RR based on ASME Code Case N-766, N-803 is necessary.

  1. Application of Quantum Dots in Biological Imaging

    Directory of Open Access Journals (Sweden)

    Shan Jin

    2011-01-01

    Full Text Available Quantum dots (QDs are a group of semiconducting nanomaterials with unique optical and electronic properties. They have distinct advantages over traditional fluorescent organic dyes in chemical and biological studies in terms of tunable emission spectra, signal brightness, photostability, and so forth. Currently, the major type of QDs is the heavy metal-containing II-IV, IV-VI, or III-V QDs. Silicon QDs and conjugated polymer dots have also been developed in order to lower the potential toxicity of the fluorescent probes for biological applications. Aqueous solubility is the common problem for all types of QDs when they are employed in the biological researches, such as in vitro and in vivo imaging. To circumvent this problem, ligand exchange and polymer coating are proven to be effective, besides synthesizing QDs in aqueous solutions directly. However, toxicity is another big concern especially for in vivo studies. Ligand protection and core/shell structure can partly solve this problem. With the rapid development of QDs research, new elements and new morphologies have been introduced to this area to fabricate more safe and efficient QDs for biological applications.

  2. FCC Universal Licensing System (ULS)

    Data.gov (United States)

    Federal Communications Commission — The Universal Licensing System allows electronic filing of applications processed by the Commission. The ULS allows you to indicate the application purpose and radio...

  3. Biocompatible quantum dots for biological applications.

    Science.gov (United States)

    Rosenthal, Sandra J; Chang, Jerry C; Kovtun, Oleg; McBride, James R; Tomlinson, Ian D

    2011-01-28

    Semiconductor quantum dots are quickly becoming a critical diagnostic tool for discerning cellular function at the molecular level. Their high brightness, long-lasting, size-tunable, and narrow luminescence set them apart from conventional fluorescence dyes. Quantum dots are being developed for a variety of biologically oriented applications, including fluorescent assays for drug discovery, disease detection, single protein tracking, and intracellular reporting. This review introduces the science behind quantum dots and describes how they are made biologically compatible. Several applications are also included, illustrating strategies toward target specificity, and are followed by a discussion on the limitations of quantum dot approaches. The article is concluded with a look at the future direction of quantum dots.

  4. Machine Learning for Biological Trajectory Classification Applications

    Science.gov (United States)

    Sbalzarini, Ivo F.; Theriot, Julie; Koumoutsakos, Petros

    2002-01-01

    Machine-learning techniques, including clustering algorithms, support vector machines and hidden Markov models, are applied to the task of classifying trajectories of moving keratocyte cells. The different algorithms axe compared to each other as well as to expert and non-expert test persons, using concepts from signal-detection theory. The algorithms performed very well as compared to humans, suggesting a robust tool for trajectory classification in biological applications.

  5. 75 FR 62592 - Applications and Amendments to Facility Operating Licenses Involving Proposed No Significant...

    Science.gov (United States)

    2010-10-12

    ... amendments to the Renewed Facility Operating Licenses include: (1) The proposed Cyber Security Plan for CCNPP... implement and maintain in effect all provisions of the Nuclear Regulatory Commission-approved Cyber Security... cyber security program. This regulation specifically requires each licensee currently licensed...

  6. 47 CFR 13.9 - Eligibility and application for new license or endorsement.

    Science.gov (United States)

    2010-10-01

    ... endorsement. 13.9 Section 13.9 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL COMMERCIAL RADIO..., the following persons are eligible to apply for commercial radio operator licenses: (1) Any person..., no person shall be eligible to be issued a commercial radio operator license when (i) The...

  7. Biological Properties and Therapeutic Applications of Propolis.

    Science.gov (United States)

    Sforcin, José M

    2016-06-01

    Propolis is a resinous material collected by bees from bud and exudates of the plants, mixed with bee enzymes, pollen and wax. In this review, the biological properties of propolis and some therapeutic applications are discussed. The same biological activities have been investigated until today, using samples from different geographic regions. Thus, the study of the biological properties of a given sample should always be associated with its chemical composition and botanical source, representing a particular sample of a given geographic area, exploring its biological potential and the role of its constituents. Efforts have been carried out to explain propolis' mechanisms of action in vivo and in vitro, but the majority of propolis' targets and actions are still unclear. The number of formulations containing propolis and patents have increased, although propolis extracts have been used deliberately with different recommendations, not always mentioning the chemical composition, vegetal source and the methods of extraction. Clinical studies will help to obtain criterious recommendations in view of the expected outcomes. Further investigation should explore the effects of common compounds found in the samples from all over the world in an attempt to standardize the research on propolis and to obtain new drugs. Copyright © 2016 John Wiley & Sons, Ltd.

  8. Marine Carotenoids: Biological Functions and Commercial Applications

    Directory of Open Access Journals (Sweden)

    José M. Vega

    2011-03-01

    Full Text Available Carotenoids are the most common pigments in nature and are synthesized by all photosynthetic organisms and fungi. Carotenoids are considered key molecules for life. Light capture, photosynthesis photoprotection, excess light dissipation and quenching of singlet oxygen are among key biological functions of carotenoids relevant for life on earth. Biological properties of carotenoids allow for a wide range of commercial applications. Indeed, recent interest in the carotenoids has been mainly for their nutraceutical properties. A large number of scientific studies have confirmed the benefits of carotenoids to health and their use for this purpose is growing rapidly. In addition, carotenoids have traditionally been used in food and animal feed for their color properties. Carotenoids are also known to improve consumer perception of quality; an example is the addition of carotenoids to fish feed to impart color to farmed salmon.

  9. Optical fibre cantilever sensor for biological application

    Science.gov (United States)

    Li, J.; Zhou, Y. X.; Patterson, G.; Shu, W. M.; Maier, R. R. J.; Fowler, R.; Hand, D. P.; MacPherson, W. N.

    2014-05-01

    Micro-cantilever sensors have shown great promise in a wide range of application are as including chemical and biological sensing. However, many of these devices are based upon a sensor `chip' that requires careful alignment between the cantilever and the read-out system, which can be challenging. Furthermore, optical interrogation typically involves a bulky free-space system. Optical fibre addressed cantilevers have been reported previously in the literature and in this paper we propose techniques to design and fabricate polymer micro-cantilevers for attachment onto the end of standard single mode fibres using laser machining. Low-cost optical sources and a fibre coupled spectrometer are employed to monitor the cantilever deflection and therefore observe biological binding between a species of interest and an activated cantilever. Proof-of-concept experiments show that the sensor is capable of detecting pathogen concentration with down to a level of 105cfu/ml.

  10. Development of probabilistic RESRAD computer codes for NRC decommissioning and license termination applications.

    Energy Technology Data Exchange (ETDEWEB)

    Chen, S. Y.; Yu, C.; Mo. T.; Trottier, C.

    2000-10-17

    In 1999, the US Nuclear Regulatory Commission (NRC) tasked Argonne National Laboratory to modify the existing RESRAD and RESRAD-BUILD codes to perform probabilistic, site-specific dose analysis for use with the NRC's Standard Review Plan for demonstrating compliance with the license termination rule. The RESRAD codes have been developed by Argonne to support the US Department of Energy's (DOEs) cleanup efforts. Through more than a decade of application, the codes already have established a large user base in the nation and a rigorous QA support. The primary objectives of the NRC task are to: (1) extend the codes' capabilities to include probabilistic analysis, and (2) develop parameter distribution functions and perform probabilistic analysis with the codes. The new codes also contain user-friendly features specially designed with graphic-user interface. In October 2000, the revised RESRAD (version 6.0) and RESRAD-BUILD (version 3.0), together with the user's guide and relevant parameter information, have been developed and are made available to the general public via the Internet for use.

  11. 78 FR 37234 - Notice of Invitation; Coal Exploration License Application NDM 105349, ND

    Science.gov (United States)

    2013-06-20

    ... Office, 5001 Southgate Drive, Billings, Montana. A written notice to participate in the exploration license should be sent to the State Director, BLM Montana State Office, 5001 Southgate Drive, Billings, MT.... BILLING CODE 4310-DN-P...

  12. 76 FR 58303 - Biweekly Notice; Applications and Amendments to Facility Operating Licenses Involving No...

    Science.gov (United States)

    2011-09-20

    ... Power Station, Unit No. 1 (DBNPS) Technical Specifications (TS) 5.5.8, ``Steam Generator (SG) Program... requirement specific to the OM holding a senior reactor operator (SRO) license. The revised BSEP, Units 1...

  13. Conducting polymer nanostructures for biological applications

    Science.gov (United States)

    Berdichevsky, Yevgeny

    A novel polypyrrole nanowire actuator was fabricated and characterized, representing a completely new approach to the design of nanoscale mechanically active components (nanomachines). This design paradigm takes advantage of the fact that unique properties of polypyrrole allow development of mechanically active nanostructures capable of operating in aqueous salt solutions with many potential applications biology and medicine. Template synthesis technique was used to electropolymerize polypyrrole nanowires in the nanoporous alumina templates. Commercial alumina filters were used both "as is" and patterned with microbeads to reduce the open pore density, along with anodized alumina prepared as a thin film on a semiconductor substrate. The ability of the nanowires to expand and contract with applied voltage was then evaluated with scanning electron microscopy and high-resolution optical microscopy. It was confirmed that the nanowires can function as nanoactuators, which is a significant advance in developing nanomechanical structures. Polypyrrole nanoactuators are electrically controlled, rather than relying on changing the chemical composition of solution, can be easily synthesized in parallel and in high numbers without requiring e-beam lithography, and can operate in aqueous salt solutions at biologically-relevant pH. Furthermore, the speed of polypyrrole actuators depends on their size due to diffusion limitations, and nanoactuators are therefore able to operate at higher speeds that micro- or macro-sized devices. The development of these nanoactuators paves the way for mimicking the function of biological actuators such as cilia, creation of controllable membranes, small particle manipulation, cellular nanomechanical probes, and many other biomedical applications. Furthermore, the same technology and process flow used for fabrication of nanoactuators was also used to create nanosensors for detection of electrochemically oxidizable neurotransmitters such as

  14. OpenCV在车牌识别中的应用%Application of OpenCV in License Plate Recognition

    Institute of Scientific and Technical Information of China (English)

    姜莉莉; 蒋潇; 陈昕怡; 殷旭东

    2014-01-01

    For license plate recognition on smart phone, application of OpenCV is studied. First, OpenCV and work flow of license plate recognition are introduced. Then detailed application of OpenCV is discussed, which is in license plate location, character segmentation and character recognition. Experimental results show that the recognition is good. The foundation is laid for further developing a vehicle information management system on smart phones such as Android.%为利用智能手机进行车牌识别,研究了OpenCV在车牌识别中的应用。首先介绍了OpenCV及车牌识别的工作流程,然后论述了OpenCV在车牌定位、字符分割和字符识别中的具体应用。实验结果表明识别效果良好,为在Android等智能手机上进一步开发车辆信息管理系统奠定了基础。

  15. 47 CFR 27.13 - License period.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false License period. 27.13 Section 27.13... COMMUNICATIONS SERVICES Applications and Licenses § 27.13 License period. (a) 2305-2320 MHz and 2345-2360 MHz... beyond the end of the license term if no broadcast service had been provided, whichever period is...

  16. 27 CFR 555.42 - License fees.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 3 2010-04-01 2010-04-01 false License fees. 555.42... EXPLOSIVES, DEPARTMENT OF JUSTICE EXPLOSIVES COMMERCE IN EXPLOSIVES Licenses and Permits § 555.42 License fees. (a) Each applicant shall pay a fee for obtaining a three year license, a separate fee...

  17. 47 CFR 24.15 - License period.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false License period. 24.15 Section 24.15 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES PERSONAL COMMUNICATIONS SERVICES Applications and Licenses General Filing Requirements § 24.15 License period. Licenses for...

  18. 27 CFR 478.42 - License fees.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 3 2010-04-01 2010-04-01 false License fees. 478.42... EXPLOSIVES, DEPARTMENT OF JUSTICE FIREARMS AND AMMUNITION COMMERCE IN FIREARMS AND AMMUNITION Licenses § 478.42 License fees. Each applicant shall pay a fee for obtaining a firearms license or...

  19. Applicability of Item Response Theory to the Korean Nurses' Licensing Examination

    Directory of Open Access Journals (Sweden)

    Geum-Hee Jeong

    2005-06-01

    Full Text Available To test the applicability of item response theory (IRT to the Korean Nurses' Licensing Examination (KNLE, item analysis was performed after testing the unidimensionality and goodness-of-fit. The results were compared with those based on classical test theory. The results of the 330-item KNLE administered to 12,024 examinees in January 2004 were analyzed. Unidimensionality was tested using DETECT and the goodness-of-fit was tested using WINSTEPS for the Rasch model and Bilog-MG for the two-parameter logistic model. Item analysis and ability estimation were done using WINSTEPS. Using DETECT, Dmax ranged from 0.1 to 0.23 for each subject. The mean square value of the infit and outfit values of all items using WINSTEPS ranged from 0.1 to 1.5, except for one item in pediatric nursing, which scored 1.53. Of the 330 items, 218 (42.7% were misfit using the two-parameter logistic model of Bilog-MG. The correlation coefficients between the difficulty parameter using the Rasch model and the difficulty index from classical test theory ranged from 0.9039 to 0.9699. The correlation between the ability parameter using the Rasch model and the total score from classical test theory ranged from 0.9776 to 0.9984. Therefore, the results of the KNLE fit unidimensionality and goodness-of-fit for the Rasch model. The KNLE should be a good sample for analysis according to the IRT Rasch model, so further research using IRT is possible.

  20. Flavonoids: Biosynthesis, Biological functions and Biotechnological applications

    Directory of Open Access Journals (Sweden)

    Maria Lorena eFalcone Ferreyra

    2012-09-01

    Full Text Available Flavonoids are widely distributed secondary metabolites with different metabolic functions in plants. The elucidation of the biosynthetic pathways, as well as their regulation by MYB, bHLH and WD40-type transcription factors, has allowed metabolic engineering of plants through the manipulation of the different final products with valuable applications. The present review describes the regulation of flavonoid biosynthesis, as well as the biological functions of flavonoids in plants, such as in defense against UV-B radiation and pathogen infection, nodulation, pollen fertility. In addition, we discuss different strategies and achievements through the genetic engineering of flavonoid biosynthesis with implication in the industry and the combinatorial biosynthesis in microorganisms by the reconstruction of the pathway to obtain high amounts of specific compounds.

  1. Application of Graph Coloring to Biological Networks

    CERN Document Server

    Khor, Susan

    2009-01-01

    We explore the application of graph coloring to biological networks, specifically protein-protein interaction (PPI) networks. First, we find that given similar conditions (i.e. number of nodes, number of links, degree distribution and clustering), fewer colors are needed to color disassortative (high degree nodes tend to connect to low degree nodes and vice versa) than assortative networks. Fewer colors create fewer independent sets which in turn imply higher concurrency potential for a network. Since PPI networks tend to be disassortative, we suggest that in addition to functional specificity and stability proposed previously by Maslov and Sneppen (Science 296, 2002), the disassortative nature of PPI networks may promote the ability of cells to perform multiple, crucial and functionally diverse tasks concurrently. Second, since graph coloring is closely related to the presence of cliques in a graph, the significance of node coloring information to the problem of identifying protein complexes, i.e. dense subg...

  2. Oligothiophenes as Fluorescent Markers for Biological Applications

    Directory of Open Access Journals (Sweden)

    Antonio Manetto

    2012-01-01

    Full Text Available This paper summarizes some of our results on the application of oligothiophenes as fluorescent markers for biological studies. The oligomers of thiophene, widely known for their semiconductor properties in organic electronics, are also fluorescent compounds characterized by chemical and optical stability, high absorbance and quantum yield. Their fluorescent emission can be easily modulated via organic synthesis by changing the number of thiophene rings and the nature of side-chains. This review shows how oligothiophenes can be derivatized with active groups such as phosphoramidite, N-hydroxysuccinimidyl and 4-sulfotetrafluorophenyl esters, isothiocyanate and azide by which the (biomolecules of interest can be covalently bound. This paper also describes how molecules such as oligonucleotides, proteins and even nanoparticles, tagged with oligothiophenes, can be used in experiments ranging from hybridization studies to imaging of fixed and living cells. Finally, a few multilabeling experiments are described.

  3. 政府数据开放许可适用研究%Research on the Application of Open Data License for the Government

    Institute of Scientific and Technical Information of China (English)

    迪莉娅

    2014-01-01

    The application of open data license plays an important role in promoting to open and use government data.The paper presents the types of open data licenses, analyzing the application conditions of foreign government open data license, and dis-cussing the important significances and strategies of establishing government open data license of China.%数据开放许可的应用对促进政府数据开放和利用具有重要的作用。文章阐述了开放数据许可的类型,分析了国外政府数据开放许可适用情况,并探讨了建立我国政府数据开放许可的重要性和策略。

  4. Graphene for Environmental and Biological Applications

    Science.gov (United States)

    Sreeprasad, T. S.; Pradeep, T.

    2012-08-01

    The latest addition to the nanocarbon family, graphene, has been proclaimed to be the material of the century. Its peculiar band structure, extraordinary thermal and electronic conductance and room temperature quantum Hall effect have all been used for various applications in diverse fields ranging from catalysis to electronics. The difficulty to synthesize graphene in bulk quantities was a limiting factor of it being utilized in several fields. Advent of chemical processes and self-assembly approaches for the synthesis of graphene analogues have opened-up new avenues for graphene based materials. The high surface area and rich abundance of functional groups present make chemically synthesized graphene (generally known as graphene oxide (GO) and reduced graphene oxide (RGO) or chemically converted graphene) an attracting candidate in biotechnology and environmental remediation. By functionalizing graphene with specific molecules, the properties of graphene can be tuned to suite applications such as sensing, drug delivery or cellular imaging. Graphene with its high surface area can act as a good adsorbent for pollutant removal. Graphene either alone or in combination with other materials can be used for the degradation or removal of a large variety of contaminants through several methods. In this review some of the relevant efforts undertaken to utilize graphene in biology, sensing and water purification are described. Most recent efforts have been given precedence over older works, although certain specific important examples of the past are also mentioned.

  5. Sleeping Beauty transposition: from biology to applications.

    Science.gov (United States)

    Narayanavari, Suneel A; Chilkunda, Shreevathsa S; Ivics, Zoltán; Izsvák, Zsuzsanna

    2017-02-01

    Sleeping Beauty (SB) is the first synthetic DNA transposon that was shown to be active in a wide variety of species. Here, we review studies from the last two decades addressing both basic biology and applications of this transposon. We discuss how host-transposon interaction modulates transposition at different steps of the transposition reaction. We also discuss how the transposon was translated for gene delivery and gene discovery purposes. We critically review the system in clinical, pre-clinical and non-clinical settings as a non-viral gene delivery tool in comparison with viral technologies. We also discuss emerging SB-based hybrid vectors aimed at combining the attractive safety features of the transposon with effective viral delivery. The success of the SB-based technology can be fundamentally attributed to being able to insert fairly randomly into genomic regions that allow stable long-term expression of the delivered transgene cassette. SB has emerged as an efficient and economical toolkit for safe and efficient gene delivery for medical applications.

  6. 76 FR 1462 - Notice Applications and Amendments to Facility Operating Licenses Involving Proposed No...

    Science.gov (United States)

    2011-01-10

    ... analysis acceptance criteria continue to be met. Power level is not an initiator of any transient or... . Entergy Operations, Inc., System Energy Resources, Inc., South Mississippi Electric Power Association, and... licensed thermal power (CLTP) of 3,898 megawatts thermal (MWt) to 4,408 MWt. Evaluations and analyses...

  7. 77 FR 6144 - Biweekly Notice; Applications and Amendments to Facility Operating Licenses Involving No...

    Science.gov (United States)

    2012-02-07

    ... modify TS Table 3.3.6.1-1, items; 1.e, ``Main Steam Tunnel Temperature-High,'' 3.f. ``Main Steam Line Tunnel Ambient Temperature-High,'' and 4.h, ``Main Steam Line Tunnel Ambient Temperature-High.'' This... of Operating Reactor Licensing, Office of Nuclear Reactor Regulation. BILLING CODE 7590-01-P...

  8. 75 FR 70350 - Liberty Natural Gas LLC, Liberty Liquefied Natural Gas (LNG) Deepwater Port License Application

    Science.gov (United States)

    2010-11-17

    ... Maritime Administration Liberty Natural Gas LLC, Liberty Liquefied Natural Gas (LNG) Deepwater Port License... receive and transfer natural gas from purpose-build LNG regasification vessels (LNGRVs) with a total cargo tank capacity of approximately 145,000 m\\3\\. The vessels would be equipped to vaporize LNG cargo...

  9. 76 FR 4417 - Liberty Natural Gas LLC, Liberty Liquefied Natural Gas (LNG) Deepwater Port License Application

    Science.gov (United States)

    2011-01-25

    ... Maritime Administration Liberty Natural Gas LLC, Liberty Liquefied Natural Gas (LNG) Deepwater Port License.... Liberty Deepwater Port would receive and transfer natural gas from purpose-built LNG regasification... equipped to vaporize LNG cargo to natural gas through onboard closed loop vaporization systems and...

  10. 75 FR 467 - Notice of Opportunity for Hearing, License Application Request of Powertech (USA) Inc. Dewey...

    Science.gov (United States)

    2010-01-05

    ... uranium by in situ recovery (ISR) extraction. By letter dated June 19, 2009, Powertech (USA) withdrew the...-Burdock In Situ Uranium Recovery Facility in Fall River and Custer Counties, SD, and Order Imposing... CONTACT: Ronald A. Burrows, Project Manager, Uranium Recovery Licensing Branch, Division of...

  11. Monovalent plasmonic nanoparticles for biological applications

    Science.gov (United States)

    Seo, Daeha; Lee, Hyunjung; Lee, Jung-uk; Haas, Thomas J.; Jun, Young-wook

    2016-03-01

    The multivalent nature of commercial nanoparticle imaging agents and the difficulties associated with producing monovalent nanoparticles challenge their use in biology, where clustering of target biomolecules can perturb dynamics of biomolecular targets. Here, we report production and purification of monovalent gold and silver nanoparticles for their single molecule imaging application. We first synthesized DNA-conjugated 20 nm and 40 nm gold and silver nanoparticles via conventional metal-thiol chemistry, yielding nanoparticles with mixed valency. By employing an anion-exchange high performance liquid chromatography (AE-HPLC) method, we purified monovalent nanoparticles from the mixtures. To allow efficient peak-separation resolution while keeping the excellent colloidal stability of nanoparticles against harsh purification condition (e.g. high NaCl), we optimized surface properties of nanoparticles by modulating surface functional groups. We characterized the monovalent character of the purified nanoparticles by hybridizing two complementary conjugates, forming dimers. Finally, we demonstrate the use of the monovalent plasmonic nanoprobes as single molecule imaging probes by tracking single TrkA receptors diffusing on the cell membrane and compare to monovalent quantum dot probes.

  12. Information theory applications for biological sequence analysis.

    Science.gov (United States)

    Vinga, Susana

    2014-05-01

    Information theory (IT) addresses the analysis of communication systems and has been widely applied in molecular biology. In particular, alignment-free sequence analysis and comparison greatly benefited from concepts derived from IT, such as entropy and mutual information. This review covers several aspects of IT applications, ranging from genome global analysis and comparison, including block-entropy estimation and resolution-free metrics based on iterative maps, to local analysis, comprising the classification of motifs, prediction of transcription factor binding sites and sequence characterization based on linguistic complexity and entropic profiles. IT has also been applied to high-level correlations that combine DNA, RNA or protein features with sequence-independent properties, such as gene mapping and phenotype analysis, and has also provided models based on communication systems theory to describe information transmission channels at the cell level and also during evolutionary processes. While not exhaustive, this review attempts to categorize existing methods and to indicate their relation with broader transversal topics such as genomic signatures, data compression and complexity, time series analysis and phylogenetic classification, providing a resource for future developments in this promising area.

  13. Detector applications in medecine and biology

    CERN Document Server

    Del Guerra, Alberto

    1995-01-01

    In recent years new diagnostic and therapeutic methods have been attracting more and more dedicated attention by the scientific community.The goal is a better understanding of the anatomy, physiology and pathology of the human being in an effort to find more appropriate medical prevention, diagnosis and therapy.Many of the achievements obtained so far derive from the use and the optimisation of detectors and techniques,which originated in the other fields of physics. The spin-off of High Energy Physics to Medical Physics has been particularly relevant in the field of detectors for medical imaging and especially for medical imaging with ionizing radiation. In this series of lectures,starting from the requests of each technique and or application I will attempt to present a survey of the detectors for medecine and biology. Various fields of medical imaging will be touched : radiology,digital radiography,mammography and radiotherapy. The capabilities of the major types of detectors (1-D and 2-D position sensitiv...

  14. 10 CFR 39.13 - Specific licenses for well logging.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Specific licenses for well logging. 39.13 Section 39.13 Energy NUCLEAR REGULATORY COMMISSION LICENSES AND RADIATION SAFETY REQUIREMENTS FOR WELL LOGGING Specific Licensing Requirements § 39.13 Specific licenses for well logging. The Commission will approve an application for a specific license for the...

  15. Applicability of Computational Systems Biology in Toxicology

    DEFF Research Database (Denmark)

    Kongsbak, Kristine Grønning; Hadrup, Niels; Audouze, Karine Marie Laure

    2014-01-01

    Systems biology as a research field has emerged within the last few decades. Systems biology, often defined as the antithesis of the reductionist approach, integrates information about individual components of a biological system. In integrative systems biology, large data sets from various sources...... and databases are used to model and predict effects of chemicals on, for instance, human health. In toxicology, computational systems biology enables identification of important pathways and molecules from large data sets; tasks that can be extremely laborious when performed by a classical literature search...... be used to establish hypotheses on links between the chemical and human diseases. Such information can also be applied for designing more intelligent animal/cell experiments that can test the established hypotheses. Here, we describe how and why to apply an integrative systems biology method...

  16. Review of biological network data and its applications.

    Science.gov (United States)

    Yu, Donghyeon; Kim, Minsoo; Xiao, Guanghua; Hwang, Tae Hyun

    2013-12-01

    Studying biological networks, such as protein-protein interactions, is key to understanding complex biological activities. Various types of large-scale biological datasets have been collected and analyzed with high-throughput technologies, including DNA microarray, next-generation sequencing, and the two-hybrid screening system, for this purpose. In this review, we focus on network-based approaches that help in understanding biological systems and identifying biological functions. Accordingly, this paper covers two major topics in network biology: reconstruction of gene regulatory networks and network-based applications, including protein function prediction, disease gene prioritization, and network-based genome-wide association study.

  17. Neutron Scattering in Biology Techniques and Applications

    CERN Document Server

    Fitter, Jörg; Katsaras, John

    2006-01-01

    The advent of new neutron facilities and the improvement of existing sources and instruments world wide supply the biological community with many new opportunities in the areas of structural biology and biological physics. The present volume offers a clear description of the various neutron-scattering techniques currently being used to answer biologically relevant questions. Their utility is illustrated through examples by some of the leading researchers in the field of neutron scattering. This volume will be a reference for researchers and a step-by-step guide for young scientists entering the field and the advanced graduate student.

  18. 37 CFR 5.11 - License for filing in a foreign country an application on an invention made in the United States...

    Science.gov (United States)

    2010-07-01

    ... foreign country an application on an invention made in the United States or for transmitting an... TRADEMARK OFFICE, DEPARTMENT OF COMMERCE GENERAL SECRECY OF CERTAIN INVENTIONS AND LICENSES TO EXPORT AND... filing in a foreign country an application on an invention made in the United States or for...

  19. Biological Applications in the Mathematics Curriculum

    Science.gov (United States)

    Marland, Eric; Palmer, Katrina M.; Salinas, Rene A.

    2008-01-01

    In this article we provide two detailed examples of how we incorporate biological examples into two mathematics courses: Linear Algebra and Ordinary Differential Equations. We use Leslie matrix models to demonstrate the biological properties of eigenvalues and eigenvectors. For Ordinary Differential Equations, we show how using a logistic growth…

  20. Applications in computer-assisted biology

    NARCIS (Netherlands)

    Nijveen, H.

    2013-01-01

    Biology is becoming a data-rich science driven by the development of high-throughput technologies like next-generation DNA sequencing. This is fundamentally changing biological research. The genome sequences of many species are becoming available, as well as the genetic variation within a species, a

  1. List of Nuclear Materials Licensing Actions Received

    Data.gov (United States)

    Nuclear Regulatory Commission — A catalog of all Materials Licensing Actions received for review. The catalog lists the name of the entity submitting the license application, their city and state,...

  2. Applicability of computational systems biology in toxicology.

    Science.gov (United States)

    Kongsbak, Kristine; Hadrup, Niels; Audouze, Karine; Vinggaard, Anne Marie

    2014-07-01

    Systems biology as a research field has emerged within the last few decades. Systems biology, often defined as the antithesis of the reductionist approach, integrates information about individual components of a biological system. In integrative systems biology, large data sets from various sources and databases are used to model and predict effects of chemicals on, for instance, human health. In toxicology, computational systems biology enables identification of important pathways and molecules from large data sets; tasks that can be extremely laborious when performed by a classical literature search. However, computational systems biology offers more advantages than providing a high-throughput literature search; it may form the basis for establishment of hypotheses on potential links between environmental chemicals and human diseases, which would be very difficult to establish experimentally. This is possible due to the existence of comprehensive databases containing information on networks of human protein-protein interactions and protein-disease associations. Experimentally determined targets of the specific chemical of interest can be fed into these networks to obtain additional information that can be used to establish hypotheses on links between the chemical and human diseases. Such information can also be applied for designing more intelligent animal/cell experiments that can test the established hypotheses. Here, we describe how and why to apply an integrative systems biology method in the hypothesis-generating phase of toxicological research.

  3. Structural Biology and Molecular Applications Research

    Science.gov (United States)

    Part of NCI's Division of Cancer Biology's research portfolio, research and development in this area focuses on enabling technologies, models, and methodologies to support basic and applied cancer research.

  4. 关联数据开放应用协议%The Open Application Licenses of Linked Data

    Institute of Scientific and Technical Information of China (English)

    张春景; 刘炜; 夏翠娟; 赵亮

    2012-01-01

    For the past few years Linked Data has been increasingly used in business, media, publication and governmental areas. In the process of publishing, consuming and re-creation of Linked Data, issues as to the ownership, the rights to use and publish, and who can benefit from Linked Data need to be appropriately addressed. Currently, the main agreements are Creative Commons family including CC BY-SA, CCO, CC BY, and CC BY-NC; the Open Data Commons family including Public Domain Dedication and License, Open Data License, Open Data Commons Attribution License and Open Government License and so on. Some suggestions on the application of Linked Data in China are put forward. 1 tab. 15 refs.%关联数据的应用正迅速发展并涉及到商业、媒体、出版、政府等领域。在关联数据发布、消费和再创造的过程中,必然涉及参与者的利益问题,需明确不同类型和归属的数据(或数据集)的所有权、发布权、使用权、收益权等。关联数据的发展迫切需要相关的许可协议。目前关联数据用到的协议主要有CC(知识共享)家族的CCBY-SA、CC0、CCBY、CCBY.NC和ODC(开放数据共用组织)的PDDL(公共领域奉献和许可),ODbL(开放数据许可)、ODC—By(开放数据共享署名许可),以及OGL(Open Government License,开放政府许可协议)等。对开放数据相关许可协议在国内的应用提出建议。表1。参考文献15。

  5. Occupational Licensing

    OpenAIRE

    Morris M. Kleiner

    2000-01-01

    The study of the regulation of occupations has a long and distinguished tradition in economics. In this paper, I present the central arguments and unresolved issues involving the costs and benefits of occupational licensing. The main benefits that are suggested for occupational licensing involve improving quality for those persons receiving the service. In contrast, the costs attributed to this labor market institution are that it restricts the supply of labor to the occupation and thereby dr...

  6. Hidden Markov processes theory and applications to biology

    CERN Document Server

    Vidyasagar, M

    2014-01-01

    This book explores important aspects of Markov and hidden Markov processes and the applications of these ideas to various problems in computational biology. The book starts from first principles, so that no previous knowledge of probability is necessary. However, the work is rigorous and mathematical, making it useful to engineers and mathematicians, even those not interested in biological applications. A range of exercises is provided, including drills to familiarize the reader with concepts and more advanced problems that require deep thinking about the theory. Biological applications are t

  7. Exploiting for medical and biological applications

    Science.gov (United States)

    Giano, Michael C.

    Biotherapeutics are an emerging class of drug composed of molecules ranging in sizes from peptides to large proteins. Due to their poor stability and mucosal membrane permeability, biotherapeutics are administered by a parenteral method (i.e., syringe, intravenous or intramuscular). Therapeutics delivered systemically often experience short half-lives. While, local administration may involve invasive surgical procedures and suffer from poor retention at the site of application. To compensate, the patient receives frequent doses of highly concentrated therapeutic. Unfortunately, the off-target side effects and discomfort associated with multiple injections results in poor patient compliance. Therefore, new delivery methods which can improve therapeutic retention, reduce the frequency of administration and may aid in decreasing the off-target side effects is a necessity. Hydrogels are a class of biomaterials that are gaining interests for tissue engineering and drug delivery applications. Hydrogel materials are defined as porous, 3-dimensional networks that are primarily composed of water. Generally, they are mechanically rigid, cytocompatible and easily chemically functionalized. Collectively, these properties make hydrogels fantastic candidates to perform as drug delivery depots. Current hydrogel delivery systems physically entrap the target therapeutic which is then subsequently released over time at the site of administration. The swelling and degradation of the material effect the diffusion of the therapy from the hydrogel, and therefore should be controlled. Although these strategies provide some regulation over therapeutic release, full control of the delivery is not achieved. Newer approaches are focused on designing hydrogels that exploit known interactions, covalently attach the therapy or respond to an external stimulus in an effort to gain improved control over the therapy's release. Unfortunately, the biotherapeutic is typically required to be chemically

  8. Synthetic Biology: Applications in the Food Sector.

    Science.gov (United States)

    Tyagi, Ashish; Kumar, Ashwani; Aparna, S V; Mallappa, Rashmi H; Grover, Sunita; Batish, Virender Kumar

    2016-08-17

    Synthetic biology also termed as "genomic alchemy" represents a powerful area of science that is based on the convergence of biological sciences with systems engineering. It has been fittingly described as "moving from reading the genetic code to writing it" as it focuses on building, modeling, designing and fabricating novel biological systems using customized gene components that result in artificially created genetic circuitry. The scientifically compelling idea of the technological manipulation of life has been advocated since long time. Realization of this idea has gained momentum with development of high speed automation and the falling cost of gene sequencing and synthesis following the completion of the human genome project. Synthetic biology will certainly be instrumental in shaping the development of varying areas ranging from biomedicine, biopharmaceuticals, chemical production, food and dairy quality monitoring, packaging, and storage of food and dairy products, bioremediation and bioenergy production, etc. However, potential dangers of using synthetic life forms have to be acknowledged and adoption of policies by the scientific community to ensure safe practice while making important advancements in the ever expanding field of synthetic biology is to be fully supported and implemented.

  9. Pesticide use and risk of end-stage renal disease among licensed pesticide applicators in the Agricultural Health Study

    Science.gov (United States)

    Lebov, Jill F.; Engel, Lawrence S.; Richardson, David; Hogan, Susan L.; Hoppin, Jane A.; Sandler, Dale P.

    2017-01-01

    Objectives Experimental studies suggest a relationship between pesticide exposure and renal impairment, but epidemiological evidence is limited. We evaluated the association between exposure to 41 specific pesticides and end-stage renal disease (ESRD) incidence in the Agricultural Health Study (AHS), a prospective cohort study of licensed pesticide applicators in Iowa and North Carolina. Methods Via linkage to the United States Renal Data System, we identified 320 ESRD cases diagnosed between enrollment (1993-1997) and December 2011 among 55,580 male licensed pesticide applicators. Participants provided pesticide use information via self-administered questionnaires. Lifetime pesticide use was defined as the product of duration and frequency of use and then modified by an intensity factor to account for differences in pesticide application practices. Cox proportional hazards models, adjusted for age and state, were used to estimate associations between ESRD and: 1) ordinal categories of intensity-weighted lifetime use of 41 pesticides, 2) poisoning and high-level pesticide exposures, and 3) pesticide exposure resulting in a medical visit or hospitalization. Results Positive exposure-response trends were observed for the herbicides alachlor, atrazine, metolachlor, paraquat, and pendimethalin, and the insecticide chlordane. More than one medical visit due to pesticide use (HR = 2.13; 95% CI: 1.17, 3.89) and hospitalization due to pesticide use (HR = 3.05; 95% CI: 1.67, 5.58) were significantly associated with ESRD. Conclusions Our findings support an association between ESRD and chronic exposure to specific pesticides and suggest pesticide exposures resulting in medical visits may increase the risk of ESRD. PMID:26177651

  10. Aspergilli: Systems biology and industrial applications

    DEFF Research Database (Denmark)

    Knuf, Christoph; Nielsen, Jens

    2012-01-01

    possible to implement systems biology tools to advance metabolic engineering. These tools include genome-wide transcription analysis and genome-scale metabolic models. Herein, we review achievements in the field and highlight the impact of Aspergillus systems biology on industrial biotechnology....... genetic modifications has enabled the use of metabolic engineering strategies. Genome sequencing of Aspergilli was originally trailing behind developments in the field of bacteria and yeasts, but with the recent availability of genome sequences for several industrially relevant Aspergilli, it has become...

  11. Photoactive molecules for applications in molecular imaging and cell biology.

    Science.gov (United States)

    Shao, Qing; Xing, Bengang

    2010-08-01

    Photoactive technology has proven successful for non-invasive regulation of biological activities and processes in living cells. With the light-directed generation of biomaterials or signals, mechanisms in cell biology can be investigated at the molecular level with spatial and temporal resolution. In this tutorial review, we aim to introduce the important applications of photoactive molecules for elucidating cell biology on aspects of protein engineering, fluorescence labelling, gene regulation and cell physiological functions.

  12. Application of computational intelligence to biology

    CERN Document Server

    Sekhar, Akula

    2016-01-01

    This book is a contribution of translational and allied research to the proceedings of the International Conference on Computational Intelligence and Soft Computing. It explains how various computational intelligence techniques can be applied to investigate various biological problems. It is a good read for Research Scholars, Engineers, Medical Doctors and Bioinformatics researchers.

  13. Recent Applications of Hidden Markov Models in Computational Biology

    Institute of Scientific and Technical Information of China (English)

    Khar Heng Choo; Joo Chuan Tong; Louxin Zhang

    2004-01-01

    This paper examines recent developments and applications of Hidden Markov Models (HMMs) to various problems in computational biology, including multiple sequence alignment, homology detection, protein sequences classification, and genomic annotation.

  14. 9 CFR 102.2 - Licenses required.

    Science.gov (United States)

    2010-01-01

    ... Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS LICENSES FOR BIOLOGICAL PRODUCTS § 102.2 Licenses required. (a) Every person who prepares biological products subject to the Virus-Serum-Toxin...

  15. Issues in the review of a license application for an above grade low-level radioactive waste disposal facility

    Energy Technology Data Exchange (ETDEWEB)

    Ringenberg, J.D. [Nebraska Dept. of Environmental Quality, NE (United States)

    1993-03-01

    In December 1987, Nebraska was selected by the Central Interstate Compact (CIC) Commission as the host state for the construction of a low-level radioactive waste disposal facility. After spending a year in the site screening process, the Compact`s developer, US Ecology, selected three sites for detailed site characterization. These sites were located in Nemaha, Nuckolls and Boyd Counties. One year later the Boyd County site was selected as the preferred site and additional site characterization studies were undertaken. On July 29, 1990, US Ecology submitted a license application to the Nebraska Department of Environmental Control (now Department of Environmental Quality-NDEQ). This paper will present issues that the NDEQ has dealt with since Nebraska`s selection as the host state for the CIC facility.

  16. 10 CFR 35.18 - License issuance.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false License issuance. 35.18 Section 35.18 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL General Information § 35.18 License issuance. (a) The Commission shall issue a license for the medical use of byproduct material if— (1) The applicant has...

  17. 75 FR 4081 - Agency Information Collection Activities; Proposed Collection; Comment Request; General Licensing...

    Science.gov (United States)

    2010-01-26

    ... application for FDA review and approval before marketing a biological product in interstate commerce. Licenses... studies are not ethical or feasible. Section 601.91(b)(3) requires applicants to prepare and provide... part 601, subpart H when human efficacy studies are not ethical or feasible (or based on evidence...

  18. Fibroblast Growth Factors: Biology, Function, and Application for Tissue Regeneration

    Directory of Open Access Journals (Sweden)

    Ye-Rang Yun

    2010-01-01

    Full Text Available Fibroblast growth factors (FGFs that signal through FGF receptors (FGFRs regulate a broad spectrum of biological functions, including cellular proliferation, survival, migration, and differentiation. The FGF signal pathways are the RAS/MAP kinase pathway, PI3 kinase/AKT pathway, and PLCγ pathway, among which the RAS/MAP kinase pathway is known to be predominant. Several studies have recently implicated the in vitro biological functions of FGFs for tissue regeneration. However, to obtain optimal outcomes in vivo, it is important to enhance the half-life of FGFs and their biological stability. Future applications of FGFs are expected when the biological functions of FGFs are potentiated through the appropriate use of delivery systems and scaffolds. This review will introduce the biology and cellular functions of FGFs and deal with the biomaterials based delivery systems and their current applications for the regeneration of tissues, including skin, blood vessel, muscle, adipose, tendon/ligament, cartilage, bone, tooth, and nerve tissues.

  19. Nanopatterning and nanoscale devices for biological applications

    CERN Document Server

    Šelimović, Seila

    2014-01-01

    ""This book is a good reference for researchers interested in realizing bio-applications based on micro- and nanostructures, where their interface with liquids and biomolecules is the key point. The most important 'players' of micro- and nano-bioengineering are considered, from DNA to proteins and cells. The work is a good merger of basic concepts and real examples of applications.""-Danilo Demarchi, Politecnico di Torino, Italy

  20. Physics and applications of microfluidics in biology.

    Science.gov (United States)

    Beebe, David J; Mensing, Glennys A; Walker, Glenn M

    2002-01-01

    Fluid flow at the microscale exhibits unique phenomena that can be leveraged to fabricate devices and components capable of performing functions useful for biological studies. The physics of importance to microfluidics are reviewed. Common methods of fabricating microfluidic devices and systems are described. Components, including valves, mixers, and pumps, capable of controlling fluid flow by utilizing the physics of the microscale are presented. Techniques for sensing flow characteristics are described and examples of devices and systems that perform bioanalysis are presented. The focus of this review is microscale phenomena and the use of the physics of the scale to create devices and systems that provide functionality useful to the life sciences.

  1. Software licenses: Stay honest!

    CERN Multimedia

    Computer Security Team

    2012-01-01

    Do you recall our article about copyright violation in the last issue of the CERN Bulletin, “Music, videos and the risk for CERN”? Now let’s be more precise. “Violating copyright” not only means the illegal download of music and videos, it also applies to software packages and applications.   Users must respect proprietary rights in compliance with the CERN Computing Rules (OC5). Not having legitimately obtained a program or the required licenses to run that software is not a minor offense. It violates CERN rules and puts the Organization at risk! Vendors deserve credit and compensation. Therefore, make sure that you have the right to use their software. In other words, you have bought the software via legitimate channels and use a valid and honestly obtained license. This also applies to “Shareware” and software under open licenses, which might also come with a cost. Usually, only “Freeware” is complete...

  2. The application of ESEM to biological samples

    Energy Technology Data Exchange (ETDEWEB)

    McGregor, J E; Donald, A M, E-mail: jem60@cam.ac.u [Cavendish Laboratory, University of Cambridge, J. J. Thomson Avenue, Cambridge CB3 OHE (United Kingdom)

    2010-07-01

    The Environmental Scanning Electron Microscope (ESEM) differs from a conventional SEM in that a differential pumping system maintains a pressure of gas (typically H{sub 2}O) in the specimen chamber whilst the gun remains at high vacuum. Ionizing collisions between electrons and these gas molecules create positive ions which drift down onto the sample neutralising specimen charge. It is therefore possible to image insulating samples without the need for metallic coating. The presence of water vapour in the chamber also means that a high relative humidity can be maintained and samples can be imaged in a hydrated state without the need for dehydration and fixation. These features suggest that ESEM could be well suited to imaging biological samples undergoing natural biological processes. We present a proof of principle study on the closure of stomatal pores in Tradescantia andersonia leaf tissue. An imaging protocol is developed and the advantages and limitations of this technique are discussed. Images of Vicia fabaleaf tissue are also presented. Challenges include minimising beam damage and reconciling the need for an adequate physiological temperature and a low gas pressure favourable for imaging, with the thermodynamic constraints on achieving a high relative humidity.

  3. Analysis Method for Licensing Application of Passive Autocatalytic Recombiner System as a Hydrogen Mitigation System of OPR-1000

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Changhyun; Sung, Jejoong; Ha, Sangjun [Korea Hydro and Nuclear Power Co. Ltd., Daejeon (Korea, Republic of); Yeo, Inseon [KEPCO Engineering and Construction Co. Ltd., Yongin (Korea, Republic of)

    2014-05-15

    As a result, for some plants, dual hydrogen mitigation systems are prepared with a combination of PARs and igniters that each system has a 100% of full capacity for hydrogen control for postulated severe accident conditions. In the original design of OPR-1000, hydrogen mitigation systems consist of a thermal recombiner and twenty glow-type igniters, which are used for design basis accident and severe accident, respectively. This paper presents an analysis method for licensing application in Korea to determine the capacity and locations of PARs for the design of a hydrogen mitigation system with PAR. A licensed analysis method of OPR-1000 has been presented to determine the capacity and locations of PAR for the design of a hydrogen mitigation system with PAR. A lumped parameter code of MAAP 4.0.6+ has been adopted to simulate various severe accident scenarios with a 26 multi-compartment containment model. Hydrogen generations were analysed and required capacity and locations of PAR were determined for six accident scenarios selected from a combination of probabilistic and deterministic considerations. A total of twenty-four PARs in the containment dome, steam generator rooms, annulus and adjacent areas was designed and the adequacy of this system has been confirmed through detailed analyses including sensitivity analyses with/without operations of safety systems such as containment heat removal systems, reactor coolant depressurization system and safety injection by accumulator, etc. Through the assessment on the possibility of global FA and DDT, it has been concluded that new PAR system with twenty-four recombiners can remove hydrogen effectively in the containment atmosphere and prevent from global FA and DDT. Further works are required in the future to develop a well-balanced analysis methodology with a combination of lumped and CFD tools focusing on the optimum locations of recombiners and local hydrogen behaviour in containment compartments.

  4. Nursing: Licensed Practical and Licensed Vocational Nurses

    Science.gov (United States)

    ... Projected Employment, 2024 Change, 2014-24 Employment by Industry Percent Numeric SOURCE: U.S. Bureau of Labor Statistics, Employment Projections program Licensed practical and licensed vocational ...

  5. Biologically Relevant Glycopeptides: Synthesis and Applications

    Science.gov (United States)

    Bennett, Clay S.; Payne, Richard J.; Koeller, Kathryn M.; Wong, Chi-Huey

    Over the past two decades interest in glycopeptides and glycoproteins has intensified, due in part to the development of new and efficient methods for the synthesis of these compounds. This includes a number of chemical and enzymatic techniques for incorporating glycosylation onto the peptide backbone as well as the introduction of powerful peptide ligation methods for the construction of glycoproteins. This review discusses these methods with a special emphasis on biologically relevant glycopeptides and glycoproteins. This includes the development of a number of antigens which hold promise as potential vaccines for HIV, cancer, or a host of other clinically important diseases. In addition the development of new antibiotics aimed at overcoming the problem of resistance will be discussed. Finally, chemical and enzymatic methods for the construction of glycopeptide mimetics will be described.

  6. 78 FR 79503 - Tennessee Valley Authority, Combined License Application for Bellefonte Units 3 and 4 Exemption...

    Science.gov (United States)

    2013-12-30

    ... (COL) application until further notice. On October 28, 2013, TVA requested an exemption from certain...) until requesting the NRC to resume its review of their COL application. The NRC staff reviewed this... report must be submitted prior to requesting the NRC resume its review of the COL application or...

  7. Biological and biomedical applications of engineered nucleases.

    Science.gov (United States)

    Pan, Yunzhi; Xiao, Li; Li, Alice S S; Zhang, Xu; Sirois, Pierre; Zhang, Jia; Li, Kai

    2013-09-01

    The development of engineered nucleases is the fruit of a new technological approach developed in the last two decades which has led to significant benefits on genome engineering, particularly on gene therapy. These applications enable efficient and specific genetic modifications via the induction of a double-strand break (DSB) in a specific genomic target sequence, followed by the homology-directed repair (HDR) or non-homologous end joining (NHEJ) pathways. In addition to the application on gene modification in cells and intact organisms, a number of recent papers have reported that this gene editing technology can be applied effectively to human diseases. With the promising data obtained using engineered endonucleases in gene therapy, it appears reasonable to expect that more diseases could be treated and even be cured in this new era of individualized medicine. This paper first brief introduces the development of engineered nucleases with a special emphasis on zinc-finger nucleases (ZFNs) and transcription activator-like effector (TALE) nucleases (TALENs), and then takes CCR5-based gene therapy as an example to discuss the therapeutic applications of engineered nucleases.

  8. [Biological characteristics of calliphoridae and its application in forensic medicine].

    Science.gov (United States)

    Zhao, Boa; Wen, Charn; Qi, Li-Li; Wang, He; Wang, Ji

    2013-12-01

    Diptera Calliphoridae is the first major kind of flies that appears on the decomposed corpses. In forensic entomology, according to the living characteristics of Calliphoridae flies, we could accurately estimate postmortem interval (PMI) in a murder or unidentified case and could provide useful clues to solve the case. This paper introduces the characteristics of the biology and morphology of Diptera Calliphoridae, and reviews the combined application of forensic entomology, molecular biology, mathematical morphology and toxicology.

  9. Mimicking biological functionality with polymers for biomedical applications

    Science.gov (United States)

    Green, Jordan J.; Elisseeff, Jennifer H.

    2016-12-01

    The vast opportunities for biomaterials design and functionality enabled by mimicking nature continue to stretch the limits of imagination. As both biological understanding and engineering capabilities develop, more sophisticated biomedical materials can be synthesized that have multifaceted chemical, biological and physical characteristics designed to achieve specific therapeutic goals. Mimicry is being used in the design of polymers for biomedical applications that are required locally in tissues, systemically throughout the body, and at the interface with tissues.

  10. Biology and applications of mycorrhizal fungi.

    Science.gov (United States)

    Sharma, S; Madan, M; Vasudevan, P

    1997-12-01

    Mycorrhizae have been shown to increase growth and yield of plants. They have been identified with both nutrient mobilization and nutrient cycling. Arbuscular (or endo-) mycorrhizae play a significant role in agriculture and most natural ecosystems, whereas ectomycorrhizae have a great potential in forestry and wasteland regeneration. The use of mycorrhizal fungi would reduce dependence on chemical fertilizers besides minimizing environmental pollution. The present review addresses the progress that there has been in the area of the ecto- and endomycorrhizae. It also examines the potential of field applications of mycorrhizal biotechnology in agriculture and forestry.

  11. 10 CFR 52.109 - Continuation of combined license.

    Science.gov (United States)

    2010-01-01

    ... Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSES, CERTIFICATIONS, AND APPROVALS FOR NUCLEAR POWER PLANTS Combined Licenses § 52.109 Continuation of combined license. Each combined license for a... continue to maintain the facility, including, where applicable, the storage, control and maintenance of...

  12. 10 CFR 51.54 - Environmental report-manufacturing license.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Environmental report-manufacturing license. 51.54 Section... report—manufacturing license. (a) Each applicant for a manufacturing license under subpart F of part 52... Environmental Report—Manufacturing License.” The environmental report must address the costs and benefits...

  13. 10 CFR 52.167 - Issuance of manufacturing license.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Issuance of manufacturing license. 52.167 Section 52.167... POWER PLANTS Manufacturing Licenses § 52.167 Issuance of manufacturing license. (a) After completing any... manufacturing license if the Commission finds that: (1) Applicable standards and requirements of the Act and...

  14. 78 FR 79018 - Request for a License To Export Deuterium

    Science.gov (United States)

    2013-12-27

    ... COMMISSION Request for a License To Export Deuterium Pursuant to 10 CFR 110.70 (b) ``Public Notice of Receipt... received the following request for an export license. Copies of the request are available electronically.... The information concerning this export license application follows. ] NRC Export License...

  15. Recent applications of synthetic biology tools for yeast metabolic engineering

    DEFF Research Database (Denmark)

    Jensen, Michael Krogh; Keasling, Jay

    2015-01-01

    to engineer microbial chemical factories has steadily decreased, improvement is still needed. Through the development of synthetic biology tools for key microbial hosts, it should be possible to further decrease the development times and improve the reliability of the resulting microorganism. Together...... with continuous decreases in price and improvements in DNA synthesis, assembly and sequencing, synthetic biology tools will rationalize time-consuming strain engineering, improve control of metabolic fluxes, and diversify screening assays for cellular metabolism. This review outlines some recently developed...... synthetic biology tools and their application to improve production of chemicals and fuels in yeast. Finally, we provide a perspective for the challenges that lie ahead....

  16. Biological applications of ultraviolet free-electron lasers

    Energy Technology Data Exchange (ETDEWEB)

    Sutherland, J.C.

    1997-10-01

    This review examines the possibilities for biological research using the three ultraviolet free-electron lasers that are nearing operational status in the US. The projected operating characteristics of major interest in biological research of the free-electron lasers at Brookhaven National Laboratory, the Thomas Jefferson National Accelerator Facility, and Duke University are presented. Experimental applications in the areas of far- and vacuum ultraviolet photophysics and photochemistry, structural biology, environmental photobiology, and medical research are discussed and the prospects for advances in these areas, based upon the characteristics of the new ultraviolet free-electron lasers, are evaluated.

  17. Structural biology applications of solid state MAS DNP NMR

    Science.gov (United States)

    Akbey, Ümit; Oschkinat, Hartmut

    2016-08-01

    Dynamic Nuclear Polarization (DNP) has long been an aim for increasing sensitivity of nuclear magnetic resonance (NMR) spectroscopy, delivering spectra in shorter experiment times or of smaller sample amounts. In recent years, it has been applied in magic angle spinning (MAS) solid-state NMR to a large range of samples, including biological macromolecules and functional materials. New research directions in structural biology can be envisaged by DNP, facilitating investigations on very large complexes or very heterogeneous samples. Here we present a summary of state of the art DNP MAS NMR spectroscopy and its applications to structural biology, discussing the technical challenges and factors affecting DNP performance.

  18. Applications of dynamical systems in biology and medicine

    CERN Document Server

    Radunskaya, Ami

    2015-01-01

    This volume highlights problems from a range of biological and medical applications that can be interpreted as questions about system behavior or control.  Topics include drug resistance in cancer and malaria, biological fluid dynamics, auto-regulation in the kidney, anti-coagulation therapy, evolutionary diversification and photo-transduction.  Mathematical techniques used to describe and investigate these biological and medical problems include ordinary, partial and stochastic differentiation equations, hybrid discrete-continuous approaches, as well as 2 and 3D numerical simulation. .

  19. Graphene nanonet for biological sensing applications.

    Science.gov (United States)

    Kim, Taekyeong; Park, Jaesung; Jin, Hye Jun; Lee, Hyungwoo; Byun, Kyung-Eun; Lee, Chang-Seuk; Kim, Kwang S; Hong, Byung Hee; Kim, Tae Hyun; Hong, Seunghun

    2013-09-20

    We report a simple but efficient method to fabricate versatile graphene nanonet (GNN)-devices. In this method, networks of V2O5 nanowires (NWs) were prepared in specific regions of single-layer graphene, and the graphene layer was selectively etched via a reactive ion etching method using the V2O5 NWs as a shadow mask. The process allowed us to prepare large scale patterns of GNN structures which were comprised of continuous networks of graphene nanoribbons (GNRs) with chemical functional groups on their edges. The GNN can be easily functionalized with biomolecules for fluorescent biochip applications. Furthermore, electrical channels based on GNN exhibited a rather high mobility and low noise compared with other network structures based on nanostructures such as carbon nanotubes, which was attributed to the continuous connection of nanoribbons in GNN structures. As a proof of concept, we built DNA sensors based on GNN channels and demonstrated the selective detection of DNA. Since our method allows us to prepare high-performance networks of GNRs over a large surface area, it should open up various practical biosensing applications.

  20. Biologically active extracts with kidney affections applications

    Science.gov (United States)

    Pascu (Neagu), Mihaela; Pascu, Daniela-Elena; Cozea, Andreea; Bunaciu, Andrei A.; Miron, Alexandra Raluca; Nechifor, Cristina Aurelia

    2015-12-01

    This paper is aimed to select plant materials rich in bioflavonoid compounds, made from herbs known for their application performances in the prevention and therapy of renal diseases, namely kidney stones and urinary infections (renal lithiasis, nephritis, urethritis, cystitis, etc.). This paper presents a comparative study of the medicinal plant extracts composition belonging to Ericaceae-Cranberry (fruit and leaves) - Vaccinium vitis-idaea L. and Bilberry (fruit) - Vaccinium myrtillus L. Concentrated extracts obtained from medicinal plants used in this work were analyzed from structural, morphological and compositional points of view using different techniques: chromatographic methods (HPLC), scanning electronic microscopy, infrared, and UV spectrophotometry, also by using kinetic model. Liquid chromatography was able to identify the specific compounds of the Ericaceae family, present in all three extracts, arbutosid, as well as specific components of each species, mostly from the class of polyphenols. The identification and quantitative determination of the active ingredients from these extracts can give information related to their therapeutic effects.

  1. Silicon quantum dots for biological applications.

    Science.gov (United States)

    Chinnathambi, Shanmugavel; Chen, Song; Ganesan, Singaravelu; Hanagata, Nobutaka

    2014-01-01

    Semiconductor nanoparticles (or quantum dots, QDs) exhibit unique optical and electronic properties such as size-controlled fluorescence, high quantum yields, and stability against photobleaching. These properties allow QDs to be used as optical labels for multiplexed imaging and in drug delivery detection systems. Luminescent silicon QDs and surface-modified silicon QDs have also been developed as potential minimally toxic fluorescent probes for bioapplications. Silicon, a well-known power electronic semiconductor material, is considered an extremely biocompatible material, in particular with respect to blood. This review article summarizes existing knowledge related to and recent research progress made in the methods for synthesizing silicon QDs, as well as their optical properties and surface-modification processes. In addition, drug delivery systems and in vitro and in vivo imaging applications that use silicon QDs are also discussed.

  2. Biological Applications of Extraordinary Electroconductance (EEC)

    Science.gov (United States)

    Tran, L. C.; Werner, F. M.; Solin, S. A.

    2014-03-01

    Rapid detection of biomolecular concentration is a fundamental goal for lab on a chip diagnostic systems. The Extraordinary Electroconductance (EEC) sensor, a stacked, AuTi-GaAs metal semiconductor hybrid structure (MSH), has been previously demonstrated to have an electric field sensitivity of 3.05V/cm in a mesoscopic-scale structure fabricated at the center of a parallel plate capacitor. In this work, we demonstrate the first successful application of EEC sensors as electrochemical detectors of molecular binding to the sensor surface. The negatively charged avidin derivative, captavidin, was applied with varying captavidin concentrations in phosphate buffered saline (PBS). The four-point measured resistance of bare EEC sensors was shown to increase by a factor of four due to captavidin binding at the sensor surface, as compared to a baseline binding assay in which the captavidin binding sites were blocked. Calculations for approximate electric field strengths introduced by a bound captavidin molecule will also presented. EEC sensors' four point measurements showed robustness and stability in spite of variations in the functional, linking layer. S.A.S. is a co-founder of and has a financial interest in PixelEXX, a start-up company whose mission is to market imaging arrays.

  3. Viability Assessment of a Repository at Yucca Mountain. Volume 4: License Application Plan and Costs

    Energy Technology Data Exchange (ETDEWEB)

    None

    1998-12-01

    defensible LA. Volume 4 is divided into seven sections. Section 2 presents a rationale and summary for the technical work to be done to develop the preclosure and postclosure safety cases that will support the compliance evaluations required for the evaluation of site suitability and for licensing. Section 2 also describes other necessary technical work, including that needed to support design decisions and development of the necessary design information. Section 3 presents a more detailed description of the technical work required to address the issues identified in Section 2. Section 3 also describes activities that will continue after submittal of the site recommendation and the LA. Examples include the drift scale heater test in the Exploratory Studies Facility (Section 3.1.4.3) and long-term waste package corrosion testing (Section 3.2.2.9). Section 4 discusses the statutory and regulatory framework for site recommendation and submittal of an LA, and describes the activities and documentation that must be completed to achieve these milestones, including the development of an EIS. Section 5 describes the numerous activities required to support program milestones, including support for completing the testing program, continuing tests as part of the performance confirmation program, and managing information and records to support regulatory and legal review. Sections 6 and 7 provide cost and schedule information for the activities planned.

  4. Biological applications of confocal fluorescence polarization microscopy

    Science.gov (United States)

    Bigelow, Chad E.

    Fluorescence polarization microscopy is a powerful modality capable of sensing changes in the physical properties and local environment of fluorophores. In this thesis we present new applications for the technique in cancer diagnosis and treatment and explore the limits of the modality in scattering media. We describe modifications to our custom-built confocal fluorescence microscope that enable dual-color imaging, optical fiber-based confocal spectroscopy and fluorescence polarization imaging. Experiments are presented that indicate the performance of the instrument for all three modalities. The limits of confocal fluorescence polarization imaging in scattering media are explored and the microscope parameters necessary for accurate polarization images in this regime are determined. A Monte Carlo routine is developed to model the effect of scattering on images. Included in it are routines to track the polarization state of light using the Mueller-Stokes formalism and a model for fluorescence generation that includes sampling the excitation light polarization ellipse, Brownian motion of excited-state fluorophores in solution, and dipole fluorophore emission. Results from this model are compared to experiments performed on a fluorophore-embedded polymer rod in a turbid medium consisting of polystyrene microspheres in aqueous suspension. We demonstrate the utility of the fluorescence polarization imaging technique for removal of contaminating autofluorescence and for imaging photodynamic therapy drugs in cell monolayers. Images of cells expressing green fluorescent protein are extracted from contaminating fluorescein emission. The distribution of meta-tetrahydroxypheny1chlorin in an EMT6 cell monolayer is also presented. A new technique for imaging enzyme activity is presented that is based on observing changes in the anisotropy of fluorescently-labeled substrates. Proof-of-principle studies are performed in a model system consisting of fluorescently labeled bovine

  5. Application of Bioinformatics and Systems Biology in Medicinal Plant Studies

    Institute of Scientific and Technical Information of China (English)

    DENG You-ping; AI Jun-mei; XIAO Pei-gen

    2010-01-01

    One important purpose to investigate medicinal plants is to understand genes and enzymes that govern the biological metabolic process to produce bioactive compounds.Genome wide high throughput technologies such as genomics,transcriptomics,proteomics and metabolomics can help reach that goal.Such technologies can produce a vast amount of data which desperately need bioinformatics and systems biology to process,manage,distribute and understand these data.By dealing with the"omics"data,bioinformatics and systems biology can also help improve the quality of traditional medicinal materials,develop new approaches for the classification and authentication of medicinal plants,identify new active compounds,and cultivate medicinal plant species that tolerate harsh environmental conditions.In this review,the application of bioinformatics and systems biology in medicinal plants is briefly introduced.

  6. Design of Functional Polyesters for Electronic and Biological Applications

    OpenAIRE

    Nelson, Ashley Marie

    2015-01-01

    Melt polymerization and novel monomers enabled the synthesis of polyesters for electronic and biological applications. Inspiration from nature and a passion for environmental preservation instigated an emphasis on the incorporation of renewable resources into polymeric materials. Critical analysis of current research surrounding bisphenol-A replacements and ion-containing segmented polyurethanes aided in identifying benchmark polymers, including limitations, challenges, and future needs. S...

  7. [Application of molecular biological techniques in Taenia identification].

    Science.gov (United States)

    Li, Yan; Liu, Hang; Yang, Yi-Mei

    2011-10-01

    The traditional identification of Taenia spp. based on morphological features of adult and cysticercus has difficulties in identifying the morphologically similar species. The recent development of molecular techniques provides more scientific ways for distinguishing Taenia species. This paper summarizes the application of molecular biological techniques in the identification of Taenia, such as analysis of DNA sequence, PCR-RFLP and LAMP.

  8. Differential scanning calorimetry techniques: applications in biology and nanoscience.

    Science.gov (United States)

    Gill, Pooria; Moghadam, Tahereh Tohidi; Ranjbar, Bijan

    2010-12-01

    This paper reviews the best-known differential scanning calorimetries (DSCs), such as conventional DSC, microelectromechanical systems-DSC, infrared-heated DSC, modulated-temperature DSC, gas flow-modulated DSC, parallel-nano DSC, pressure perturbation calorimetry, self-reference DSC, and high-performance DSC. Also, we describe here the most extensive applications of DSC in biology and nanoscience.

  9. 75 FR 57521 - Biweekly Notice Applications and Amendments to Facility Operating Licenses Involving No...

    Science.gov (United States)

    2010-09-21

    ... applicable acceptance criteria. The safety analyses for these accidents have been performed at the EPU power... is consistent with the safety analysis assumptions. The proposed RPS and ESFAS Limiting Safety System... (CST),'' resulting from (1) modifications to the AFW system to support requirements for transients...

  10. 76 FR 56483 - Applications and Amendments to Facility Operating Licenses Involving Proposed No Significant...

    Science.gov (United States)

    2011-09-13

    ... MWt. Nuclear steam supply system and balance-of-plant systems, components and analyses that could be... applicable analysis acceptance criteria are still met with the proposed changes. Power level is an input assumption to equipment design and accident analyses, but it is not a transient or accident...

  11. 75 FR 15701 - Georgia Power Company; Notice of Application for Amendment of License and Soliciting Comments...

    Science.gov (United States)

    2010-03-30

    ... inspection: a. Application Type: Non-project use of project lands and waters. b. Project No: 1951-169. c... Street, NE., Washington, DC 20426. For more information on how to submit these types of filings, please... wooden sea wall; and dredging of 212 cubic yards of sediment. The amount of concrete and asphalt...

  12. 77 FR 8288 - Applications and Amendments to Facility Operating Licenses Involving Proposed No Significant...

    Science.gov (United States)

    2012-02-14

    ... value shall be added to the cycle-specific SLMCPR value. This adder is applicable to SLO , which is.... The proposed change to the MCPR SLs accounts for the 0.02 adder specified in the NRC Safety Evaluation... revision to the MCPR SLs accounts for the 0.02 adder specified in the NRC Safety Evaluation limitations...

  13. 75 FR 4111 - Biweekly Notice; Applications and Amendments to Facility Operating Licenses Involving No...

    Science.gov (United States)

    2010-01-26

    ... resistance to tube pullout due to the pressure induced forces, with applicable safety factors applied... revises testing acceptance criteria for the existing Indian Point 2 Control Room filtration system in... criteria for the existing Control Room filtration system. The proposed change does not involve...

  14. 77 FR 48512 - Northern Indiana Public Service Company; Notice of Application for Amendment of License and...

    Science.gov (United States)

    2012-08-14

    ... 0.25 feet of elevation 612.45 NGVD. The U.S. Fish and Wildlife Service and the Indiana Department of...: A copy of the application is available for inspection and reproduction at the Commission's Public... available for inspection and reproduction at the address in item (h) above. m. Individuals desiring to...

  15. 75 FR 17170 - Notice of Opportunity To Request a Hearing for the License Application From International...

    Science.gov (United States)

    2010-04-05

    ... Fluorine Products, Inc., for a Fluoride Extraction and Uranium Deconversion Facility in Lea County NM and... INFORMATION CONTACT: Matt Bartlett, Project Manager, Advanced Fuel Cycle, Enrichment, and Uranium Conversion... application from International Isotopes Fluorine Products, Inc. (IIFP), for a proposed fluoride extraction...

  16. 75 FR 52039 - Biweekly Notice Applications and Amendments to Facility Operating Licenses Involving No...

    Science.gov (United States)

    2010-08-24

    ... application for amendment which is available for public inspection at the Commission's PDR, located at One..., ``Both Isolation Actuation Instrument Channels Inoperable'' (Agencywide Documents Access and Management... restricts the maximum fuel rod average burnup. Deletion of this condition would allow the maximum fuel...

  17. 75 FR 32509 - Notice Applications and Amendments to Facility Operating Licenses Involving Proposed No...

    Science.gov (United States)

    2010-06-08

    ... amendment action, see the application for amendment which is available for public inspection at the... and channel surveillance requirements associated with average power range monitor simulated thermal... the RPV head is removed, any gaseous release due to fuel damage from any accident or transient...

  18. 75 FR 76000 - Alabama Power Company; Notice of Application for Amendment of License and Soliciting Comments...

    Science.gov (United States)

    2010-12-07

    ..., access channel, and landscaping. Existing facilities that would be re-built in place at their current dimensions include a floating fuel dock and two floating courtesy docks. Proposed new facilities include a... of the application is available for inspection and reproduction at the Commission's Public...

  19. 18 CFR 16.9 - Applications for new licenses and nonpower licenses for projects subject to sections 14 and 15 of...

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Applications for new... Section 16.9 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF... served on all competing applicants no later than the date specified in the notice issued under...

  20. Click Chemistry Mediated Functionalization of Vertical Nanowires for Biological Applications.

    Science.gov (United States)

    Vutti, Surendra; Schoffelen, Sanne; Bolinsson, Jessica; Buch-Månson, Nina; Bovet, Nicolas; Nygård, Jesper; Martinez, Karen L; Meldal, Morten

    2016-01-11

    Semiconductor nanowires (NWs) are gaining significant importance in various biological applications, such as biosensing and drug delivery. Efficient and controlled immobilization of biomolecules on the NW surface is crucial for many of these applications. Here, we present for the first time the use of the Cu(I) -catalyzed alkyne-azide cycloaddition and its strain-promoted variant for the covalent functionalization of vertical NWs with peptides and proteins. The potential of the approach was demonstrated in two complementary applications of measuring enzyme activity and protein binding, which is of general interest for biological studies. The attachment of a peptide substrate provided NW arrays for the detection of protease activity. In addition, green fluorescent protein was immobilized in a site-specific manner and recognized by antibody binding to demonstrate the proof-of-concept for the use of covalently modified NWs for diagnostic purposes using minute amounts of material.

  1. Application of synthetic biology in cyanobacteria and algae

    Directory of Open Access Journals (Sweden)

    Bo eWang

    2012-09-01

    Full Text Available Cyanobacteria and algae are becoming increasingly attractive cell factories for producing renewable biofuels and chemicals due to their ability to capture solar energy and CO2 and their relatively simple genetic background for genetic manipulation. Increasing research efforts from the synthetic biology approach have been made in recent years to modify cyanobacteria and algae for various biotechnological applications. In the article, we critically review recent progresses in developing genetic tools for characterizing or manipulating cyanobacteria and algae, the applications of genetically modified strains for synthesizing renewable products such as biofuels and chemicals. In addition, the emergent challenges in the development and application of synthetic biology for cyanobacteria and algae are also discussed.

  2. Supramolecular Assemblies Responsive to Biomolecules toward Biological Applications.

    Science.gov (United States)

    Shigemitsu, Hajime; Hamachi, Itaru

    2015-10-01

    Stimuli-responsive supramolecular assemblies consisting of small molecules are attractive functional materials for biological applications such as drug delivery, medical diagnosis, enzyme immobilization, and tissue engineering. By use of their dynamic and reversible properties, many supramolecular assemblies responsive to a variety of biomolecules have been designed and synthesized. This review focuses on promising strategies for the construction of such dynamic supramolecular assemblies and their functions. While studies of biomolecule-responsive supramolecular assemblies have mainly been performed in vitro, it has recently been demonstrated that some of them can work in live cells. Supramolecular assemblies now open up new avenues in chemical biology and biofunctional materials.

  3. An introduction to stochastic processes with applications to biology

    CERN Document Server

    Allen, Linda J S

    2010-01-01

    An Introduction to Stochastic Processes with Applications to Biology, Second Edition presents the basic theory of stochastic processes necessary in understanding and applying stochastic methods to biological problems in areas such as population growth and extinction, drug kinetics, two-species competition and predation, the spread of epidemics, and the genetics of inbreeding. Because of their rich structure, the text focuses on discrete and continuous time Markov chains and continuous time and state Markov processes.New to the Second EditionA new chapter on stochastic differential equations th

  4. Licensing failure in the European decentralised procedure

    NARCIS (Netherlands)

    Langedijk, Joris; Ebbers, Hans C; Mantel-Teeuwisse, Aukje K; Kruger-Peters, Alexandra G; Leufkens, Hubert G M

    2016-01-01

    The majority of the licensing applications in the European Union are submitted via the decentralised procedure. Little is known about licensing failure (i.e. refusal or withdrawal of a marketing authorisation application) in the EU decentralised procedure compared to the EU centralised procedure and

  5. 2015 Business Licenses

    Data.gov (United States)

    City of Jackson, Mississippi — This data displays all business license information for the year of 2015. This information details license classifications and status. This information will updated...

  6. Licensed Healthcare Facilities

    Data.gov (United States)

    California Department of Resources — The Licensed Healthcare Facilities point layer represents the locations of all healthcare facilities licensed by the State of California, Department of Health...

  7. Texas site selection and licensing status

    Energy Technology Data Exchange (ETDEWEB)

    Avant, R.V. Jr.

    1989-11-01

    Texas has identified a potential site in Hudspeth County in far West Texas near the town of Fort Hancock. Over the past year the Texas Low-Level Radioactive Waste Disposal Authority has been conducting detailed geology, hydrology, meteorology, soils, and flora and fauna evaluations. An authorization by the Board of Directors of the Authority to proceed with a license application, assuming that the detailed evaluation indicates that the site is suitable, is expected by September. A prototype license has been prepared in anticipation of the order to proceed with licensing, and the formal license application is expected to be submitted to the Texas Department of Health-Bureau of Radiation Control in December, meeting the license application milestone. Although site selection processes in all siting areas across the country have experienced organized opposition, El Paso County has funded a particularly well-organized, well-financed program to legally and technically stop consideration of the Fort Hancock site prior to the licensing process. Many procedural, regulatory, and technical issues have been raised which have required responses from the Authority in order to proceed with licensing. This has provided a unique perspective of what to expect from well-organized opposition at the licensing stage. This paper presents an update on the Texas siting activity with detailed information on the site evaluation and license application. Experience of dealing with issues raised by opposition relating to NRC guidelines and rules is also discussed.

  8. [Development of Zn(2+) selective fluorescent probes for biological applications].

    Science.gov (United States)

    Hagimori, Masayori

    2013-01-01

    Zn(2+) is an essential element for life and is known to play important roles in biological processes including gene expression, apoptosis, enzyme regulation, immune system and neurotransmission. To investigate physiological roles of free or chelatable Zn(2+) in living cells, Zn(2+)-selective fluorescent probes are valuable tools. A variety of fluorescent probes based on quinoline, BF2 chelated dipyrromethene, fluorescein, etc. has been developed recently. In principle, such tools can provide useful information about zinc biology. However, most of the fluorescent probes presented so far possess a fluorescent core and a separate part for binding to Zn(2+) within the molecule, so that the molecular weight is usually large and the molecules are hydrophobic. As a result, the applications of such molecules in biological systems often face difficulties. Therefore, we need to develop a new class of fluorescent probes for Zn(2+) with improved molecular characteristics. If the initial core structure is small enough, the fluorescent probes may still be molecular weight below 500 with desirable physico-chemical properties, even after the modifications. In this review, we described novel low-molecular-weight fluorescent probes for Zn(2+) based on pyridine-pyridone. Small modification of pyridine-pyridone core structure brought about a marked improvement such as aqueous solubility, affinity toward Zn(2+), and fluorescence ON/OFF switching. Fluorescence images of Zn(2+) in cells showed that the pyridine-pyridone probe can be used in biological applications.

  9. Applications of large-scale density functional theory in biology

    Science.gov (United States)

    Cole, Daniel J.; Hine, Nicholas D. M.

    2016-10-01

    Density functional theory (DFT) has become a routine tool for the computation of electronic structure in the physics, materials and chemistry fields. Yet the application of traditional DFT to problems in the biological sciences is hindered, to a large extent, by the unfavourable scaling of the computational effort with system size. Here, we review some of the major software and functionality advances that enable insightful electronic structure calculations to be performed on systems comprising many thousands of atoms. We describe some of the early applications of large-scale DFT to the computation of the electronic properties and structure of biomolecules, as well as to paradigmatic problems in enzymology, metalloproteins, photosynthesis and computer-aided drug design. With this review, we hope to demonstrate that first principles modelling of biological structure-function relationships are approaching a reality.

  10. Research Applications of Proteolytic Enzymes in Molecular Biology

    Directory of Open Access Journals (Sweden)

    József Tőzsér

    2013-11-01

    Full Text Available Proteolytic enzymes (also termed peptidases, proteases and proteinases are capable of hydrolyzing peptide bonds in proteins. They can be found in all living organisms, from viruses to animals and humans. Proteolytic enzymes have great medical and pharmaceutical importance due to their key role in biological processes and in the life-cycle of many pathogens. Proteases are extensively applied enzymes in several sectors of industry and biotechnology, furthermore, numerous research applications require their use, including production of Klenow fragments, peptide synthesis, digestion of unwanted proteins during nucleic acid purification, cell culturing and tissue dissociation, preparation of recombinant antibody fragments for research, diagnostics and therapy, exploration of the structure-function relationships by structural studies, removal of affinity tags from fusion proteins in recombinant protein techniques, peptide sequencing and proteolytic digestion of proteins in proteomics. The aim of this paper is to review the molecular biological aspects of proteolytic enzymes and summarize their applications in the life sciences.

  11. Silicon nanoparticles: applications in cell biology and medicine

    OpenAIRE

    O’Farrell, Norah; Houlton, Andrew; Horrocks, Benjamin R.

    2006-01-01

    In this review, we describe the synthesis, physical properties, surface functionalization, and biological applications of silicon nanoparticles (also known as quantum dots). We compare them against current technologies, such as fluorescent organic dyes and heavy metal chalcogenide-based quantum dots. In particular, we examine the many different methods that can be used to both create and modify these nanoparticles and the advantages they may have over current technologies that have stimulated...

  12. Laser heating of dielectric particles for medical and biological applications

    OpenAIRE

    2016-01-01

    We consider the general problem of laser pulse heating of a spherical dielectric particle embedded in a liquid. The discussed range of the problem parameters is typical for medical and biological applications. We focus on the case, when the heat diffusivity in the particle is of the same order of magnitude as that in the fluid. We perform quantitative analysis of the heat transfer equation based on interplay of four characteristic scales of the problem, namely the particle radius, the charact...

  13. 1,2,4-triazine derivatives: Synthesis and biological applications

    Directory of Open Access Journals (Sweden)

    Mohammad Arshad

    2014-04-01

    Full Text Available 1,2,4-Triazines are the six membered heterocyclic compounds possessing three nitrogen in its structure with general formula C3H3N3. 1,2,4-Triazines and its derivatives have been found to exhibit the variety of biological applications such as antifungal, anti-HIV, anticancer, antiinflammatory, analgesic and antihypertensive, cardiotonic, neuroleptic, nootropic, antihistaminergic, tuberculostatic, antiviral, anti-protozoal, estrogen receptor modulators, antimalarial, cyclin-dependent kinase inhibitors, antimicrobial and antiparasitic activities.

  14. Applications of electrochemistry and nanotechnology in biology and medicine II

    CERN Document Server

    Eliaz, Noam

    2011-01-01

    The study of electrochemical nanotechnology has emerged as researchers apply electrochemistry to nanoscience and nanotechnology. These two related volumes in the Modern Aspects of Electrochemistry Series review recent developments and breakthroughs in the specific application of electrochemistry and nanotechnology to biology and medicine. Internationally renowned experts contribute chapters that address both fundamental and practical aspects of several key emerging technologies in biomedicine, such as the processing of new biomaterials, biofunctionalization of surfaces, characterization of bio

  15. Photoluminescent ZnO Nanoparticles and Their Biological Applications

    Directory of Open Access Journals (Sweden)

    Zheng-Yong Zhang

    2015-05-01

    Full Text Available During the past decades, numerous achievements concerning luminescent zinc oxide nanoparticles (ZnO NPs have been reported due to their improved luminescence and good biocompatibility. The photoluminescence of ZnO NPs usually contains two parts, the exciton-related ultraviolet (UV emission and the defect-related visible emission. With respect to the visible emission, many routes have been developed to synthesize and functionalize ZnO NPs for the applications in detecting metal ions and biomolecules, biological fluorescence imaging, nonlinear multiphoton imaging, and fluorescence lifetime imaging. As the biological applications of ZnO NPs develop rapidly, the toxicity of ZnO NPs has attracted more and more attention because ZnO can produce the reactive oxygen species (ROS and release Zn2+ ions. Just as a coin has two sides, both the drug delivery and the antibacterial effects of ZnO NPs become attractive at the same time. Hence, in this review, we will focus on the progress in the synthetic methods, luminescent properties, and biological applications of ZnO NPs.

  16. Preparation and application of various nanoparticles in biology and medicine

    Directory of Open Access Journals (Sweden)

    Vardan Gasparyan

    2013-02-01

    Full Text Available The present paper considers prospects for application of various nanoparticles in biology and medicine. Here are presented data on preparation of gold and silver nanoparticles, and effects of shape of these nanoparticles on their optical properties. Application of these nanoparticles in diagnostics, for drug delivery and therapy, and preparation of magnetic nanoparticles from iron and cobalt salts are also discussed. Application of these nanoparticles as magnetic resonance imaging (MRI contrast agents and as vehicles for drug delivery, and preparation of quantum dots and their application as prospective nanoparticles for multiplex analysis and for visualization of cellular processes will be tackled. Finally, prospects for new types of nanocomposites (metallic nano-shells will be not overlooked.

  17. 78 FR 44105 - Monroe Hydro, LLC; Notice of Intent to File License Application, Filing of Pre-Application...

    Science.gov (United States)

    2013-07-23

    ... Commission's regulations h. Potential Applicant Contact: Gia Schneider, Natel Energy, 2175 Monarch Street, Alameda, CA 94501; (510) 342-5269; email -gia@natelenergy.com . i. FERC Contact: Adam Beeco at (202)...

  18. 78 FR 69402 - Lock 7 Hydro Partners, LLC; Notice of Application for Amendment of License and Soliciting...

    Science.gov (United States)

    2013-11-19

    ... generating unit No. 2. The runner replacement would increase the installed and hydraulic capacities of the... installed capacity would change from 2,040 kW to 2,210 kW and its hydraulic capacity from 2,229 cfs to 2,386... relate to project works which are the subject of the license amendment. Agencies may obtain copies of...

  19. 76 FR 62398 - Northern States Power Company; Notice of Application To Amend License and Accepted for Filing...

    Science.gov (United States)

    2011-10-07

    ... increase by 1,400 kW [from 36,000 kW to 39,000 kW] and the maximum hydraulic capacity would increase by 720... relate to project works which are the subject of the license surrender. Agencies may obtain copies of...

  20. 78 FR 5508 - Vogtle Electric Generating Plant, Units 3 and 4; Application and Amendment to Combined Licenses...

    Science.gov (United States)

    2013-01-25

    ... requirements for shear reinforcement spacing in the AP1000 basemat does not impact the support, design, or...) to clarify the requirements for shear reinforcement spacing in the nuclear island basemat below the... reinforcement in the basemat below the auxiliary building. Before issuance of the proposed license...

  1. 78 FR 5505 - Turkey Point, Units 3 and 4; Application and Amendment to Facility Operating License Involving...

    Science.gov (United States)

    2013-01-25

    ... and Management System (ADAMS): You may access publicly available documents online in the NRC Library...: License amendment request; opportunity to comment, request a hearing and petition for leave to intervene... pH control system consisting of ten stainless steel baskets loaded with NaTB but do not...

  2. 77 FR 23241 - Lock+ Hydro Friends Fund XXX, LLC; Notice of Intent To File License Application, Filing of Pre...

    Science.gov (United States)

    2012-04-18

    ... Federal Energy Regulatory Commission Lock+ Hydro Friends Fund XXX, LLC; Notice of Intent To File License... Friends Fund XXX, LLC. e. Name of Project: New Cumberland Locks and Dam Hydroelectric Project. f. Location... XXX, c/o Hydro Green Energy, LLC, 900 Oakmont Lane, Suite 310, Westmont, IL 60559; (877) 556-6566...

  3. Liquid Exfoliation of Layered Transition Metal Dichalcogenides for Biological Applications.

    Science.gov (United States)

    Nguyen, Emily P; Daeneke, Torben; Zhuiykov, Serge; Kalantar-Zadeh, Kourosh

    2016-06-02

    Known to possess distinctive properties that differ greatly from their bulk form, layered two-dimensional materials have been extensively studied and incorporated into many versatile applications ranging from optoelectronics to sensors. For biomedical research, two-dimensional transition metal dichalcogenides (2D TMDs) have garnered much interest as they have been shown to exhibit relatively low toxicity, high stability in aqueous environments, and the ability to adhere to biological materials such as proteins. These materials are promising candidates, demonstrating potential applications in biosensing, cell imaging, diagnostics, and therapeutics. Preparation and exfoliation of 2D TMDs play an important part in these various applications as their properties are heavily dependent on the number of layers and lateral size. Described in this article are protocols for the liquid exfoliation of 2D TMDs from their bulk materials. Additional protocols are also provided for functionalizing or modifying the surface of the exfoliated 2D TMDs. © 2016 by John Wiley & Sons, Inc.

  4. Compulsory licensing of patents in India.

    Science.gov (United States)

    Chaudhry, Rahul

    2016-09-01

    This article deals with compulsory licensing scenarios in India, provides a background of relevant provisions in the Patents Act and examines how these provisions are Trade-Related Aspects of Intellectual Property Rights compliant. This article further discusses the procedure followed by India in granting a compulsory license, provides an overview of compulsory license applications filed in India to date and judicial precedence regarding the same. This article also highlights how compulsory licensing is a great safeguard that balances the interests of the innovators and the public at large.

  5. Application of enriched stable isotopes as tracers in biological systems

    DEFF Research Database (Denmark)

    Stürup, Stefan; Hansen, Helle Rüsz; Gammelgaard, Bente

    2008-01-01

    The application of enriched stable isotopes of minerals and trace elements as tracers in biological systems is a rapidly growing research field that benefits from the many new developments in inorganic mass spectrometric instrumentation, primarily within inductively coupled plasma mass spectrometry...... (ICP-MS) instrumentation, such as reaction/collision cell ICP-MS and multicollector ICP-MS with improved isotope ratio measurement and interference removal capabilities. Adaptation and refinement of radioisotope tracer experiment methodologies for enriched stable isotope experiments......, and the development of new methodologies coupled with more advanced compartmental and mathematical models for the distribution of elements in living organisms has enabled a broader use of enriched stable isotope experiments in the biological sciences. This review discusses the current and future uses of enriched...

  6. Classical and spatial stochastic processes with applications to biology

    CERN Document Server

    Schinazi, Rinaldo B

    2014-01-01

    The revised and expanded edition of this textbook presents the concepts and applications of random processes with the same illuminating simplicity as its first edition, but with the notable addition of substantial modern material on biological modeling. While still treating many important problems in fields such as engineering and mathematical physics, the book also focuses on the highly relevant topics of cancerous mutations, influenza evolution, drug resistance, and immune response. The models used elegantly apply various classical stochastic models presented earlier in the text, and exercises are included throughout to reinforce essential concepts. The second edition of Classical and Spatial Stochastic Processes is suitable as a textbook for courses in stochastic processes at the advanced-undergraduate and graduate levels, or as a self-study resource for researchers and practitioners in mathematics, engineering, physics, and mathematical biology. Reviews of the first edition: An appetizing textbook for a f...

  7. 76 FR 32866 - Cable Landing Licenses; Correction

    Science.gov (United States)

    2011-06-07

    ... Systems Agency in the regulations that we published in the Federal Register of January 14, 2002, 67 FR... COMMISSION 47 CFR Part 1 Cable Landing Licenses; Correction AGENCY: Federal Communications Commission. ACTION... streamlined processing of cable landing license applications. Need for Correction As published, the...

  8. Pulsed electrical discharges for medicine and biology techniques, processes, applications

    CERN Document Server

    Kolikov, Victor

    2015-01-01

    This book presents the application of pulsed electrical discharges in water and water dispersions of metal nanoparticles in medicine (surgery, dentistry, and oncology), biology, and ecology. The intensive electrical and shock waves represent a novel technique to destroy viruses and this way to  prepare anti-virus vaccines. The method of pulsed electrical discharges in water allows to decontaminate water from almost all known bacteria and spores of fungi being present in human beings. The nanoparticles used are not genotoxic and mutagenic. This book is useful for researchers and graduate students.

  9. [Applications of molecular biology in the wine industry].

    Science.gov (United States)

    Ramón, D; González-Candelas, L; Pérez-González, J A; González, R; Ventura, L; Sánchez-Torres, P; Vallés, S; Piñaga, F; Gallego, M V; Fernández-Espinar, M T

    1995-03-01

    Population dynamics of natural and inoculated industrial wine fermentations have been studied by using a simple molecular biology technique based on mitochondrial DNA restriction analysis profile. The predominance of the inoculated strain in the inoculated fermentations is obvious. A genetic transformation system has been developed for an industrial wine yeast strain named T73. By using this technique, different fungal hydrolases in this industrial strain have been expressed. Problems and benefits of the application of recombinant DNA techniques in wine yeast strains are also discussed here.

  10. Nonlinear plasmonic imaging techniques and their biological applications

    Science.gov (United States)

    Deka, Gitanjal; Sun, Chi-Kuang; Fujita, Katsumasa; Chu, Shi-Wei

    2017-01-01

    Nonlinear optics, when combined with microscopy, is known to provide advantages including novel contrast, deep tissue observation, and minimal invasiveness. In addition, special nonlinearities, such as switch on/off and saturation, can enhance the spatial resolution below the diffraction limit, revolutionizing the field of optical microscopy. These nonlinear imaging techniques are extremely useful for biological studies on various scales from molecules to cells to tissues. Nevertheless, in most cases, nonlinear optical interaction requires strong illumination, typically at least gigawatts per square centimeter intensity. Such strong illumination can cause significant phototoxicity or even photodamage to fragile biological samples. Therefore, it is highly desirable to find mechanisms that allow the reduction of illumination intensity. Surface plasmon, which is the collective oscillation of electrons in metal under light excitation, is capable of significantly enhancing the local field around the metal nanostructures and thus boosting up the efficiency of nonlinear optical interactions of the surrounding materials or of the metal itself. In this mini-review, we discuss the recent progress of plasmonics in nonlinear optical microscopy with a special focus on biological applications. The advancement of nonlinear imaging modalities (including incoherent/coherent Raman scattering, two/three-photon luminescence, and second/third harmonic generations that have been amalgamated with plasmonics), as well as the novel subdiffraction limit imaging techniques based on nonlinear behaviors of plasmonic scattering, is addressed.

  11. Human Metabolic Network: Reconstruction, Simulation, and Applications in Systems Biology

    Science.gov (United States)

    Wu, Ming; Chan, Christina

    2012-01-01

    Metabolism is crucial to cell growth and proliferation. Deficiency or alterations in metabolic functions are known to be involved in many human diseases. Therefore, understanding the human metabolic system is important for the study and treatment of complex diseases. Current reconstructions of the global human metabolic network provide a computational platform to integrate genome-scale information on metabolism. The platform enables a systematic study of the regulation and is applicable to a wide variety of cases, wherein one could rely on in silico perturbations to predict novel targets, interpret systemic effects, and identify alterations in the metabolic states to better understand the genotype-phenotype relationships. In this review, we describe the reconstruction of the human metabolic network, introduce the constraint based modeling approach to analyze metabolic networks, and discuss systems biology applications to study human physiology and pathology. We highlight the challenges and opportunities in network reconstruction and systems modeling of the human metabolic system. PMID:24957377

  12. Ortholog prediction of the Aspergillus genus applicable for synthetic biology

    DEFF Research Database (Denmark)

    Rasmussen, Jane Lind Nybo; Vesth, Tammi Camilla; Theobald, Sebastian

    The Aspergillus genus contains leading industrial microorganisms, excelling in producing bioactive compounds and enzymes. Using synthetic biology and bioinformatics, we aim to re-engineer these organisms for applications within human health, pharmaceuticals, environmental engineering, and food...... production. In this project, we compare the genomes of +300 species from the Aspergillus genus to generate a high-resolution pan-genomic map, representing genetic diversity spanning ~200 million years. We are identifying genes specific to species and clades to allow for guilt-by-association-based mapping......-directional hits. The result is orthologous protein families describing the genomic and functional features of individual species, clades and the core/pan genome of Aspergillus; and applicable to genotype-to-phenotype analyses in other microbial genera....

  13. Applications of Chitin and Its Derivatives in Biological Medicine

    Directory of Open Access Journals (Sweden)

    Moon-Moo Kim

    2010-12-01

    Full Text Available Chitin and its derivatives—as a potential resource as well as multiple functional substrates—have generated attractive interest in various fields such as biomedical, pharmaceutical, food and environmental industries, since the first isolation of chitin in 1811. Moreover, chitosan and its chitooligosaccharides (COS are degraded products of chitin through enzymatic and acidic hydrolysis processes; and COS, in particular, is well suited for potential biological application, due to the biocompatibility and nontoxic nature of chitosan. In this review, we investigate the current bioactivities of chitin derivatives, which are all correlated with their biomedical properties. Several new and cutting edge insights here may provide a molecular basis for the mechanism of chitin, and hence may aid its use for medical and pharmaceutical applications.

  14. Micro-segmented flow applications in chemistry and biology

    CERN Document Server

    Cahill, Brian

    2014-01-01

    The book is dedicated to the method and application potential of micro segmented flow. The recent state of development of this powerful technique is presented in 12 chapters by leading researchers from different countries. In the first section, the principles of generation and manipulation of micro-fluidic segments are explained. In the second section, the micro continuous-flow synthesis of different types of nanomaterials is shown as a typical example for the use of advantages of the technique in chemistry. In the third part, the particular importance of the technique in biotechnical applications is presented demonstrating the progress for miniaturized cell-free processes, for molecular biology and DNA-based diagnostis and sequencing as well as for the development of antibiotics and the evaluation of toxic effects in medicine and environment.

  15. Review on thin-film transistor technology, its applications, and possible new applications to biological cells

    Science.gov (United States)

    Tixier-Mita, Agnès; Ihida, Satoshi; Ségard, Bertrand-David; Cathcart, Grant A.; Takahashi, Takuya; Fujita, Hiroyuki; Toshiyoshi, Hiroshi

    2016-04-01

    This paper presents a review on state-of-the-art of thin-film transistor (TFT) technology and its wide range of applications, not only in liquid crystal displays (TFT-LCDs), but also in sensing devices. The history of the evolution of the technology is first given. Then the standard applications of TFT-LCDs, and X-ray detectors, followed by state-of-the-art applications in the field of chemical and biochemical sensing are presented. TFT technology allows the fabrication of dense arrays of independent and transparent microelectrodes on large glass substrates. The potential of these devices as electrical substrates for biological cell applications is then described. The possibility of using TFT array substrates as new tools for electrical experiments on biological cells has been investigated for the first time by our group. Dielectrophoresis experiments and impedance measurements on yeast cells are presented here. Their promising results open the door towards new applications of TFT technology.

  16. Nanowire-Based Sensors for Biological and Medical Applications.

    Science.gov (United States)

    Wang, Zongjie; Lee, Suwon; Koo, Kyo; Kim, Keekyoung

    2016-04-01

    Nanomaterials such as nanowires, carbon nanotubes, and nanoparticles have already led to breakthroughs in the field of biological and medical sensors. The quantum size effects of the nanomaterials and their similarity in size to natural and synthetic nanomaterials are anticipated to improve sensor sensitivity dramatically. Nanowires are considered as key nanomaterials because of their electrical controllability for accurate measurement, and chemical-friendly surface for various sensing applications. This review covers the working principles and fabrication of silicon nanowire sensors. Furthermore, we review their applications for the detection of viruses, biomarkers, and DNA, as well as for drug discovery. Advances in the performance and functionality of nanowire sensors are also surveyed to highlight recent progress in this area. These advances include the improvements in reusability, sensitivity in high ionic strength solvent, long-term stability, and self-powering. Overall, with the advantages of ultra-sensitivity and the ease of fabrication, it is expected that nanowires will contribute significantly to the development of biological and medical sensors in the immediate future.

  17. Application of proteomics to ecology and population biology.

    Science.gov (United States)

    Karr, T L

    2008-02-01

    Proteomics is a relatively new scientific discipline that merges protein biochemistry, genome biology and bioinformatics to determine the spatial and temporal expression of proteins in cells, tissues and whole organisms. There has been very little application of proteomics to the fields of behavioral genetics, evolution, ecology and population dynamics, and has only recently been effectively applied to the closely allied fields of molecular evolution and genetics. However, there exists considerable potential for proteomics to impact in areas related to functional ecology; this review will introduce the general concepts and methodologies that define the field of proteomics and compare and contrast the advantages and disadvantages with other methods. Examples of how proteomics can aid, complement and indeed extend the study of functional ecology will be discussed including the main tool of ecological studies, population genetics with an emphasis on metapopulation structure analysis. Because proteomic analyses provide a direct measure of gene expression, it obviates some of the limitations associated with other genomic approaches, such as microarray and EST analyses. Likewise, in conjunction with associated bioinformatics and molecular evolutionary tools, proteomics can provide the foundation of a systems-level integration approach that can enhance ecological studies. It can be envisioned that proteomics will provide important new information on issues specific to metapopulation biology and adaptive processes in nature. A specific example of the application of proteomics to sperm ageing is provided to illustrate the potential utility of the approach.

  18. Cerium oxide nanoparticles: green synthesis and biological applications

    Science.gov (United States)

    Charbgoo, Fahimeh; Ahmad, Mansor Bin; Darroudi, Majid

    2017-01-01

    CeO2 nanoparticles (NPs) have shown promising approaches as therapeutic agents in biology and medical sciences. The physicochemical properties of CeO2-NPs, such as size, agglomeration status in liquid, and surface charge, play important roles in the ultimate interactions of the NP with target cells. Recently, CeO2-NPs have been synthesized through several bio-directed methods applying natural and organic matrices as stabilizing agents in order to prepare biocompatible CeO2-NPs, thereby solving the challenges regarding safety, and providing the appropriate situation for their effective use in biomedicine. This review discusses the different green strategies for CeO2-NPs synthesis, their advantages and challenges that are to be overcome. In addition, this review focuses on recent progress in the potential application of CeO2-NPs in biological and medical fields. Exploiting biocompatible CeO2-NPs may improve outcomes profoundly with the promise of effective neurodegenerative therapy and multiple applications in nanobiotechnology. PMID:28260887

  19. 76 FR 57720 - Intent To Grant an Exclusive Patent License

    Science.gov (United States)

    2011-09-16

    ... Department of the Air Force Intent To Grant an Exclusive Patent License SUMMARY: Pursuant to the provisions... exclusive license in any right, title and interest the United States Air Force has in: U.S. Patent... license for the invention described in this patent application will be granted unless a written...

  20. Establishment of licensing process for development reactors

    Energy Technology Data Exchange (ETDEWEB)

    Jo, Jong Chull; Yune, Young Gill; Kim, Woong Sik (and others)

    2006-02-15

    A study on licensing processes for development reactors has been performed to prepare the licensing of development reactors developed in Korea. The contents and results of the study are summarized as follows. The licensing processes for nuclear reactors in Korea, U.S.A., Japan, France, U.K., Canada, and IAEA were surveyed and analyzed to obtain technical bases necessary for establishing licensing processes applicable to development reactors in Korea. Based on the technical bases obtained the above analysis, the purpose, power output, and design characteristics of development reactors were analyzed in detail. The analysis results suggested that development reactors should be classified as a new reactor category (called as 'development reactor') separated from the current reactor categories such as the research reactor and the power reactor. Therefore, it is proposed to establish a new reactor category classified as 'development reactor' for the development reactors. And licensing processes, including licensing technical requirements, licensing document requirements, and other regulatory requirements, were also proposed for the development reactors. In order to institutionalize the licensing processes developed in this study, it is necessary to revise the current laws. Therefore, draft provisions of Atomic Energy Act, Enforcement Decree of the Atomic Energy Act, and Enforcement Regulation of the Atomic Energy Act have been developed for the preparation of the future legalization of the licensing processes proposed for the development reactors. Conclusively, a proposal of licensing processes and draft provisions of laws have been developed for the development reactors. The results proposed in this study can be applied directly to the licensing of the future development reactors. Furthermore, they will also contribute to establishing successfully the licensing processes of the development reactors.

  1. Terahertz spectroscopy for chemicals and biological sensing applications

    Science.gov (United States)

    Liu, Hai-Bo

    Terahertz (THz) radiation offers innovative sensing and imaging technologies that can provide information unavailable through other conventional electromagnetic techniques. With the advancement of THz technologies, THz sensing will impact a broad range of areas. This thesis focuses on the use of THz spectroscopy for sensing applications including explosives detection, pharmaceutical identification and biological characterization. Using both a THz time-domain spectroscopy (THz-TDS) system and a Fourier transform far-infrared spectrometer (FT-FIR), a THz spectral database of explosives and related compounds (ERCs) in the range of 0.1-20 THz was established. The transmission measurements show good agreement with the diffuse reflectance measurements, which are more feasible for practical applications. Density Functional Theory was employed to calculate structures and vibrational modes of several important ERCs and the calculated spectra are in good accordance with the experimental data in the 3-20 THz range. The detection and identification of the explosive RDX using diffusely reflected THz waves were also demonstrated. THz-TDS was applied successfully for pharmaceutical study, such as investigating drug interactions, as well as identifying hydrated and anhydrous drugs, based upon the intermolecular vibrational modes of drug substances. Dehydrations and complex solid state reactions of pharmaceutical materials were studied with THz-TDS and the reaction kinetics was successfully probed. These investigations have opened new avenues for using THz technologies in pharmaceutical science and industry. THz spectra of amino acids, purines and other biomolecules were recorded. Most of these solid-state biocompounds have THz spectral features in the 0.1-3.0 THz range. THz spectroscopy of solid-state proteins and bioactive protein micro suspensions in organic media was studied and their THz absorption features may reflect their collective vibrational modes which could be used to

  2. Application of a new operating license for the Finnish FiR 1 reactor and the change of generation of the reactor personnel

    Energy Technology Data Exchange (ETDEWEB)

    Salmenhaara, Seppo; Auterinen, Iiro [VTT Technical Research Centre of Finland, Otaniemi, Espoo (Finland)

    2008-10-29

    The FiR 1 epithermal BNCT facility is a TRIGA Mark II reactor: 250 kW; 15 kg U containing 3 kg {sup 235}U (20% enrichment) in the special TRIGA uranium-zirconium hydride fuel (8-12 w% U, 91% Zr, 1% H); epithermal neutrons are created by the FLUENTAL{sup TM} neutron moderator; Neutron collimation: Bi + Li-Poly cone; epithermal neutron flux: 1.1 10{sup 9} /cm{sup 2}s; fast neutron dose: 2 Gy/10{sup 13} cm{sup -2}. The schedule of the Operating License Application is as follows: - 2009 decision to apply a new license; - 2010 preparation of the documents needed for the application; - 2011 the documents will be checked by the authorities and at the end of the year the new license should be granted by the Government; - 2012-2016 probable period of the new license The supplementary documents to the application for an operating license are: 1. Details of the site; 2. The quality and maximum amounts of the nuclear material 3. An outline of the technical operating principles and arrangements whereby the safety has been ensured; 4. A description of the safety principles that have been observed, and an evaluation of the fulfillment of the principles; 5. A description of the measures to restrict the burden caused by the nuclear facility on the environment; 6. The expertise available to the applicant and the operating organization; 7. Plans for arranging nuclear waste management. The applicant submits to the Radiation and Nuclear Safety Authority: 1. The final safety analysis report; 2. A probabilistic safety analysis; 3. A quality assurance programme for the operation of the nuclear facility; 4. Technical specifications; 5. A summary programme for periodic inspections; 6. A description of the arrangements for physical protection and emergencies; 7. A description on how to arrange the safeguards that are necessary to prevent the proliferation of nuclear weapons; 8. Administrative rules; 9. A programme for radiation monitoring in the environment. Reactor key persons and the

  3. Natural analog studies: Licensing perspective

    Energy Technology Data Exchange (ETDEWEB)

    Bradbury, J.W. [Nuclear Regulatory Commission, Washington, DC (United States)

    1995-09-01

    This report describes the licensing perspective of the term {open_quotes}natural analog studies{close_quotes} as used in CFR Part 60. It describes the misunderstandings related to its definition which has become evident during discussions at the U.S Nuclear Regulatory Commission meetings and tries to clarify the appropriate applications of natural analog studies to aspects of repository site characterization.

  4. 10 CFR 72.6 - License required; types of licenses.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false License required; types of licenses. 72.6 Section 72.6... Provisions § 72.6 License required; types of licenses. (a) Licenses for the receipt, handling, storage, and transfer of spent fuel or high-level radioactive waste are of two types: general and specific. Licenses...

  5. NRC licensing requirements: DOD options

    Energy Technology Data Exchange (ETDEWEB)

    Pike, W.J.; O' Reilly, P.D.

    1982-09-01

    This report describes the licensing process (both safety and environmental) that would apply if the Department of Defense (DOD) chooses to obtain licenses from the US Nuclear Regulatory Commission (NRC) for using nuclear energy for power and luminous sources. The specific nuclear energy sources being considered include: small or medium-size nuclear power reactors; radioisotopic thermoelectric generators with /sup 90/Sr or /sup 238/Pu; radioisotopic dynamic electric generators with /sup 90/Sr or /sup 238/Pu; and applications of radioisotopes for luminous sources (lights) with /sup 3/H, /sup 85/Kr, or /sup 147/Pm. The steps of the licensing process are summarized in the following sections, with particular attention given to the schedule and level of effort necessary to support the process.

  6. State Licenses & Permits

    Data.gov (United States)

    Small Business Administration — Starting a business? Confused about whether you need a business license or permit? Virtually every business needs some form of license or permit to operate legally....

  7. Technology licensing in China

    DEFF Research Database (Denmark)

    Wang, Yuandi; Li-Ying, Jason; Chen, Jin

    2015-01-01

    We explore the landscape of technology licensing among Chinese entities in the period 2000–12, using a unique database on technological licensing from the State Intellectual Property Office of China. We find that: first, among Chinese licensee organizations, firms have dominated in terms...... of the number of licensed technologies; second, the geographical distribution of licensed technologies among the provinces has gradually reached a new quantitative balance; third, utility models are the most popular technologies to be licensed and the majority of technology licensing in China has been between...... Chinese entities, and most transactions have been local within provinces; and finally, Chinese firms have gradually in-licensed newer and newer technologies, but the technologies in-licensed from foreign sources are by no means state-of-the-art. We make several suggestions for innovation policy...

  8. Improved antireflection coated microspheres for biological applications of optical tweezers

    Science.gov (United States)

    Ferro, Valentina; Sonnberger, Aaron; Abdosamadi, Mohammad K.; McDonald, Craig; Schäffer, Erik; McGloin, David

    2016-09-01

    The success of optical tweezers in cellular biology1 is in part due to the wide range of forces that can be applied, from femto- to hundreds of pico-Newtons; nevertheless extending the range of applicable forces to the nanoNewton regime opens access to a new set of phenomena that currently lie beyond optical manipulation. A successful approach to overcome the conventional limits on trapping forces involves the optimization of the trapped probes. Jannasch et al.2 demonstrated that an anti-reflective shell of nanoporous titanium dioxide (aTiO2, nshell = 1.75) on a core particle made out of titanium dioxide in the anatase phase (cTiO2, ncore = 2.3) results in trappable microspheres capable to reach forces above 1 nN. Here we present how the technique can be further improved by coating the high refractive index microspheres with an additional anti-reflective shell made out of silica (SiO2). This external shell not only improves the trap stability for microspheres of different sizes, but also enables the use of functionalization techniques already established for commercial silica beads in biological experiments. We are also investigating the use of these new microspheres as probes to measure adhesion forces between intercellular adhesion molecule 1 (ICAM-1) and lymphocyte function-associated antigen 1 (LFA-1) in effector T-Cells and will present preliminary results comparing standard and high-index beads.

  9. Models to Study NK Cell Biology and Possible Clinical Application.

    Science.gov (United States)

    Zamora, Anthony E; Grossenbacher, Steven K; Aguilar, Ethan G; Murphy, William J

    2015-08-03

    Natural killer (NK) cells are large granular lymphocytes of the innate immune system, responsible for direct targeting and killing of both virally infected and transformed cells. NK cells rapidly recognize and respond to abnormal cells in the absence of prior sensitization due to their wide array of germline-encoded inhibitory and activating receptors, which differs from the receptor diversity found in B and T lymphocytes that is due to the use of recombination-activation gene (RAG) enzymes. Although NK cells have traditionally been described as natural killers that provide a first line of defense prior to the induction of adaptive immunity, a more complex view of NK cells is beginning to emerge, indicating they may also function in various immunoregulatory roles and have the capacity to shape adaptive immune responses. With the growing appreciation for the diverse functions of NK cells, and recent technological advancements that allow for a more in-depth understanding of NK cell biology, we can now begin to explore new ways to manipulate NK cells to increase their clinical utility. In this overview unit, we introduce the reader to various aspects of NK cell biology by reviewing topics ranging from NK cell diversity and function, mouse models, and the roles of NK cells in health and disease, to potential clinical applications. © 2015 by John Wiley & Sons, Inc.

  10. Fluid Mechanics of Biological Surfaces and their Technological Application

    Science.gov (United States)

    Bechert, D. W.; Bruse, M.; Hage, W.; Meyer, R.

    A survey is given on fluid-dynamic effects caused by the structure and properties of biological surfaces. It is demonstrated that the results of investigations aiming at technological applications can also provide insights into biophysical phenomena. Techniques are described both for reducing wall shear stresses and for controlling boundary-layer separation. (a) Wall shear stress reduction was investigated experimentally for various riblet surfaces including a shark skin replica. The latter consists of 800 plastic model scales with compliant anchoring. Hairy surfaces are also considered, and surfaces in which the no-slip condition is modified. Self-cleaning surfaces such as that of lotus leaves represent an interesting option to avoid fluid-dynamic deterioration by the agglomeration of dirt. An example of technological implementation is discussed for riblets in long-range commercial aircraft. (b) Separation control is also an important issue in biology. After a few brief comments on vortex generators, the mechanism of separation control by bird feathers is described in detail. Self-activated movable flaps (=artificial bird feathers) represent a high-lift system enhancing the maximum lift of airfoils by about 20%. This is achieved without perceivable deleterious effects under cruise conditions. Finally, flight experiments on an aircraft with laminar wing and movable flaps are presented.

  11. Applications of Inorganic Chemistry in Biology: An Interdisciplinary Graduate Course

    Science.gov (United States)

    Farrell, Nicholas; Ross, Paul; Roat, Rosette M.

    1998-06-01

    Inorganic chemistry faculty at Virginia Commonwealth University (VCU) are offering an advanced, interdisciplinary, graduate course entitled "Applications of Inorganic Chemistry in Biology". The course utilizes examples from bioinorganic chemistry to introduce advanced topics in synthesis, structural analysis, and analytical methods that are practiced by inorganic chemists. Emphasis is placed on the structure and function of trace and ultratrace transition metals in biological systems and on the use of metals for medicinal purposes. Instrumental techniques such as electron paramagnetic resonance, Mössbauer spectroscopy, and X-ray crystallography are explained in the detail necessary to familiarize students with their use for analysis of bioinorganic systems and their models. Students have take-home examinations during the term and write a term paper describing a metalloprotein whose X-ray structure data is listed in Brookhaven protein data base. The paper follows the same course pattern of classroom discussion of a bioinorganic system, concentrating on the coordination geometry and nearest neighbor contacts of the metal-binding site in the protein, substrate binding site, and relevance to the metalloprotein or enzyme function, mechanism of action of the enzyme or protein, spectroscopic studies on the metal-binding site, and model studies for the protein's metal-binding site. The instructors conclude that their basic goals for the course - introduction to advanced inorganic chemistry topics using bioinorganic examples with emphasis on primary literature sources and computer-assisted displays - are being accomplished.

  12. Biology and Industrial Applications of Chlorella: Advances and Prospects.

    Science.gov (United States)

    Liu, Jin; Chen, Feng

    2016-01-01

    Chlorella represents a group of eukaryotic green microalgae that has been receiving increasing scientific and commercial interest. It possesses high photosynthetic ability and is capable of growing robustly under mixotrophic and heterotrophic conditions as well. Chlorella has long been considered as a source of protein and is now industrially produced for human food and animal feed. Chlorella is also rich in oil, an ideal feedstock for biofuels. The exploration of biofuel production by Chlorella is underway. Chlorella has the ability to fix carbon dioxide efficiently and to remove nutrients of nitrogen and phosphorous, making it a good candidate for greenhouse gas biomitigation and wastewater bioremediation. In addition, Chlorella shows potential as an alternative expression host for recombinant protein production, though challenges remain to be addressed. Currently, omics analyses of certain Chlorella strains are being performed, which will help to unravel the biological implications of Chlorella and facilitate the future exploration of industrial applications.

  13. DNA confinement in nanochannels: physics and biological applications

    DEFF Research Database (Denmark)

    Reisner, Walter; Pedersen, Jonas Nyvold; Austin, Robert H

    2012-01-01

    in nanochannels, creating a linear unscrolling of the genome along the channel for analysis. We will first review the fundamental physics of DNA nanochannel confinement—including the effect of varying ionic strength—and then discuss recent applications of these systems to genomic mapping. Apart from the intense...... direct assessment of the genome in its native state). In this review, we will discuss how the information contained in genomic-length single DNA molecules can be accessed via physical confinement in nanochannels. Due to self-avoidance interactions, DNA molecules will stretch out when confined...... biological interest in extracting linear sequence information from elongated DNA molecules, from a physics view these systems are fascinating as they enable probing of single-molecule conformation in environments with dimensions that intersect key physical length-scales in the 1 nm to 100μm range. (Some...

  14. 86Y based PET radiopharmaceuticals: radiochemistry and biological applications.

    Science.gov (United States)

    Nayak, Tapan K; Brechbiel, Martin W

    2011-09-01

    Development of targeted radionuclide therapy with (90)Y labeled antibodies and peptides has gained momentum in the past decade due to the successes of (90)Y-ibritumomab tiuxetan and (90)Y-DOTA-Phe(1)-Tyr(3)-octreotide in treatment of cancer. (90)Y is a pure β(-)-emitter and cannot be imaged for patient-specific dosimetry which is essential for pre-therapeutic treatment planning and accurate absorbed dose estimation in individual patients to mitigate radiation related risks. This review article describes the utility of (86)Y, a positron emitter (33%) with a 14.7-h half-life that can be imaged by positron emission tomography and used as an isotopically matched surrogate radionuclide for (90)Y radiation doses estimations. This review discusses various aspects involved in the development of (86)Y labeled radiopharmaceuticals with the specific emphasis on the radiochemistry and biological applications with antibodies and peptides.

  15. Surface treatments for biological, chemical and physical applications

    CERN Document Server

    Karaman, Mustafa

    2017-01-01

    A step-by-step guide to the topic with a mix of theory and practice in the fields of biology, chemistry and physics. Straightforward and well-structured, the first chapter introduces fundamental aspects of surface treatments, after which examples from nature are given. Subsequent chapters discuss various methods to surface modification, including chemical and physical approaches, followed by the characterization of the functionalized surfaces. Applications discussed include the lotus effect, diffusion barriers, enzyme immobilization and catalysis. Finally, the book concludes with a look at future technology advances. Throughout the text, tutorials and case studies are used for training purposes to grant a deeper understanding of the topic, resulting in an essential reference for students as well as for experienced engineers in R&D.

  16. Modular Verification of Interactive Systems with an Application to Biology

    Directory of Open Access Journals (Sweden)

    P. Milazzo

    2011-01-01

    Full Text Available We propose sync-programs, an automata-based formalism for the description of biological systems, and a modular verification technique for such a formalism that allows properties expressed in the universal fragment of CTL to be verified on suitably chosen fragments of models, rather than on whole models. As an application we show the modelling of the lac operon regulation process and the modular verification of some properties. Verification of properties is performed by using the NuSMV model checker and we show that by applying our modular verification technique we can verify properties in shorter times than those necessary to verify the same properties in the whole model.

  17. Stochasticity in processes fundamentals and applications to chemistry and biology

    CERN Document Server

    Schuster, Peter

    2016-01-01

    This book has developed over the past fifteen years from a modern course on stochastic chemical kinetics for graduate students in physics, chemistry and biology. The first part presents a systematic collection of the mathematical background material needed to understand probability, statistics, and stochastic processes as a prerequisite for the increasingly challenging practical applications in chemistry and the life sciences examined in the second part. Recent advances in the development of new techniques and in the resolution of conventional experiments at nano-scales have been tremendous: today molecular spectroscopy can provide insights into processes down to scales at which current theories at the interface of physics, chemistry and the life sciences cannot be successful without a firm grasp of randomness and its sources. Routinely measured data is now sufficiently accurate to allow the direct recording of fluctuations. As a result, the sampling of data and the modeling of relevant processes are doomed t...

  18. Normal mode analysis and applications in biological physics

    Energy Technology Data Exchange (ETDEWEB)

    Dykeman, Eric C; Sankey, Otto F [Department of Physics, Center for Biological Physics, Arizona State University, Tempe, AZ 85287-1504 (United States)

    2010-10-27

    Normal mode analysis has become a popular and often used theoretical tool in the study of functional motions in enzymes, viruses, and large protein assemblies. The use of normal modes in the study of these motions is often extremely fruitful since many of the functional motions of large proteins can be described using just a few normal modes which are intimately related to the overall structure of the protein. In this review, we present a broad overview of several popular methods used in the study of normal modes in biological physics including continuum elastic theory, the elastic network model, and a new all-atom method, recently developed, which is capable of computing a subset of the low frequency vibrational modes exactly. After a review of the various methods, we present several examples of applications of normal modes in the study of functional motions, with an emphasis on viral capsids. (topical review)

  19. Various On-Chip Sensors with Microfluidics for Biological Applications

    Directory of Open Access Journals (Sweden)

    Hun Lee

    2014-09-01

    Full Text Available In this paper, we review recent advances in on-chip sensors integrated with microfluidics for biological applications. Since the 1990s, much research has concentrated on developing a sensing system using optical phenomena such as surface plasmon resonance (SPR and surface-enhanced Raman scattering (SERS to improve the sensitivity of the device. The sensing performance can be significantly enhanced with the use of microfluidic chips to provide effective liquid manipulation and greater flexibility. We describe an optical image sensor with a simpler platform for better performance over a larger field of view (FOV and greater depth of field (DOF. As a new trend, we review consumer electronics such as smart phones, tablets, Google glasses, etc. which are being incorporated in point-of-care (POC testing systems. In addition, we discuss in detail the current optical sensing system integrated with a microfluidic chip.

  20. Licensing of Practices

    CERN Document Server

    2001-01-01

    All practices are licensed by Radiation Protection Centre (RPC). RPC verifies if license seeker is prepares to work with sources in an adequate and safe manner. Types of licences defined in the Republic of Lithuania are listed. Data on the number of licensees and of licenses issued in 1999-2001 is presented.

  1. A novel POSS-coated quantum dot for biological application

    Directory of Open Access Journals (Sweden)

    Rizvi SB

    2012-08-01

    Full Text Available Sarwat B Rizvi,1 Lara Yildirimer,1 Shirin Ghaderi,1 Bala Ramesh,1 Alexander M Seifalian,1,2 Mo Keshtgar1,21UCL Centre for Nanotechnology and Regenerative Medicine, Division of Surgery and Interventional Science, University College London, United Kingdom; 2Royal Free Hampstead NHS Trust Hospital, London, United KingdomAbstract: Quantum dots (QDs are fluorescent semiconductor nanocrystals that have the potential for major advancements in the field of nanomedicine through their unique photophysical properties. They can potentially be used as fluorescent probes for various biomedical imaging applications, including cancer localization, detection of micrometastasis, image guided surgery, and targeted drug delivery. Their main limitation is toxicity, which requires a biologically compatible surface coating to shield the toxic core from the surrounding environment. However, this leads to an increase in QD size that may lead to problems of excretion and systemic sequestration. We describe a one pot synthesis, characterization, and in vitro cytotoxicity of a novel polyhedral oligomeric silsesquioxane (POSS-coated CdTe-cored QD using mercaptosuccinic acid (MSA and D-cysteine as stabilizing agents. Characterization was performed using transmission electron microscopy Fourier transform infrared spectroscopy, and photoluminescence studies. POSS-coated QDs demonstrated high colloidal stability and enhanced photostability on high degrees of ultraviolet (UV excitation compared to QDs coated with MSA and D-cysteine alone (P value < 0.05. In vitro toxicity studies showed that both POSS and MSA-QDs were significantly less toxic than ionized salts of Cd+2 and Te-2. Confocal microscopy confirmed high brightness of POSS-QDs in cells at both 1 and 24 hours, indicating that these QDs are rapidly taken up by cells and remain photostable in a biological environment. We therefore conclude that a POSS coating confers biological compatibility, photostability, and colloidal

  2. Polyhydroyalkanoates: from Basic Research and Molecular Biology to Application

    Directory of Open Access Journals (Sweden)

    Amro Abd alFattah Amara

    2010-09-01

    Full Text Available This review describes the Polyhydroxyalkanoate (PHA, an intracellular biodegradable microbial polymer. PHAs is formed from different types of three hydroxyalkanoic acids monomers, each unit forms an ester bond with the hydroxyl group of the other one and the hydroxyl substituted carbon has R configuration. The C-3 atom in β position is branched with at least one carbon atom in the form of methyl group (C1 to thirteen carbons in the form of tridecyl (C13. This alkyl side chain is not necessarily saturated. PHAs are biosynthesized through regulated pathways by specific enzymes. PHAs are accumulated in bacterial cells from soluble to insoluble form as storage materials inside the inclusion bodies during unbalanced nutrition or to save organisms from reducing equivalents. PHAs are converted again to soluble components by PHAs depolymerases and the degraded materials enter various metabolic pathways. Until now, four classes of enzymes responsible for PHAs polymerization are known. PHAs were well studied regarding their promising applications, physical, chemical and biological properties. PHAs are biodegradable, biocompatible, have good material properties, renewable and can be used in many applications. The most limiting factor in PHAs commercialization is their high cost compared to the petroleum plastics. This review highlights the new knowledge and that established by the pioneers in this field as well as the factors, which affect PHAs commercialization.

  3. Guided Bone Regeneration: biological principle and therapeutic applications.

    Science.gov (United States)

    Retzepi, Maria; Donos, N

    2010-06-01

    The Guided Bone Regeneration (GBR) treatment concept advocates that regeneration of osseous defects is predictably attainable via the application of occlusive membranes, which mechanically exclude non-osteogenic cell populations from the surrounding soft tissues, thereby allowing osteogenic cell populations originating from the parent bone to inhabit the osseous wound. The present review discusses the evolution of the GBR biological rationale and therapeutic concept over the last two decades. Further, an overview of the GBR research history is provided with specific focus on the evidence available on its effectiveness and predictability in promoting the regeneration of critical size cranio-maxillo-facial defects, the neo-osteogenesis potential and the reconstruction of atrophic alveolar ridges before, or in conjunction with, the placement of dental implants. The authors conclude that future research should focus on (a) the investigation of the molecular mechanisms underlying the wound healing process following GBR application; (b) the identification of site and patient related factors which impact on the effectiveness and predictability of GBR therapy and (c) the evaluation of the pathophysiology of the GBR healing process in the presence of systemic conditions potentially affecting the skeletal system.

  4. Heparin-Mimicking Polymers: Synthesis and Biological Applications

    Science.gov (United States)

    2016-01-01

    Heparin is a naturally occurring, highly sulfated polysaccharide that plays a critical role in a range of different biological processes. Therapeutically, it is mostly commonly used as an injectable solution as an anticoagulant for a variety of indications, although it has also been employed in other forms such as coatings on various biomedical devices. Due to the diverse functions of this polysaccharide in the body, including anticoagulation, tissue regeneration, anti-inflammation, and protein stabilization, and drawbacks of its use, analogous heparin-mimicking materials are also widely studied for therapeutic applications. This review focuses on one type of these materials, namely, synthetic heparin-mimicking polymers. Utilization of these polymers provides significant benefits compared to heparin, including enhancing therapeutic efficacy and reducing side effects as a result of fine-tuning heparin-binding motifs and other molecular characteristics. The major types of the various polymers are summarized, as well as their applications. Because development of a broader range of heparin-mimicking materials would further expand the impact of these polymers in the treatment of various diseases, future directions are also discussed. PMID:27739666

  5. Functionalized nanoparticles for biological imaging and detection applications

    Science.gov (United States)

    Mei, Bing C.

    Semiconductor quantum dots (QDs) and gold nanoparticles (AuNPs) have gained tremendous attention in the last decade as a result of their size-dependent spectroscopic properties. These nanoparticles have been a subject of intense study to bridge the gap between macroscopic and atomic behavior, as well as to generate new materials for novel applications in therapeutics, biological sensing, light emitting devices, microelectronics, lasers, and solar cells. One of the most promising areas for the use of these nanoparticles is in biotechnology, where their size-dependent optical properties are harnessed for imaging and sensing applications. However, these nanoparticles, as synthesized, are often not stable in aqueous media and lack simple and reliable means of covalently linking to biomolecules. The focus of this work is to advance the progress of these nanomaterials for biotechnology by synthesizing them, characterizing their optical properties and rendering them water-soluble and functional while maintaining their coveted optical properties. QDs were synthesized by an organometallic chemical procedure that utilizes coordinating solvents to provide brightly luminescent nanoparticles. The optical interactions of these QDs were studied as a function of concentration to identify particle size-dependent optimal concentrations, where scattering and indirection excitation are minimized and the amount light observed per particle is maximized. Both QDs and AuNPs were rendered water-soluble and stable in a broad range of biologically relevant conditions by using a series of ligands composed of dihydrolipoic acid (DHLA) appended to poly(ethylene glycol) methyl ether. By studying the stability of the surface modified AuNPs, we revealed some interesting information regarding the role of the surface ligand on the nanoparticle stability (i.e. solubility in high salt concentration, resistance to dithiothreitol competition and cyanide decomposition). Furthermore, the nanoparticles

  6. Standard Review Plan for the review of a license application for a low-level radioactive waste disposal facility. Revision 3

    Energy Technology Data Exchange (ETDEWEB)

    1994-04-01

    The Standard Review Plan (SRP) (NUREG-1200) provides guidance to staff reviewers in the Office of Nuclear Material Safety and Safeguards who perform safety reviews of applications to construct and operate low-level radioactive waste disposal facilities. The SRP ensures the quality and uniformity of the staff reviews and presents a well-defined base from which to evaluate proposed changes in the scope and requirements of the staff reviews. The SRP makes information about the regulatory licensing process widely available and serves to improve the understanding of the staff`s review process by interested members of the public and the industry. Each individual SRP addresses the responsibilities of persons performing the review, the matters that are reviewed, the Commission`s regulations and acceptance criteria necessary for the review, how the review is accomplished, the conclusions that are appropriate, and the implementation requirements.

  7. Dielectric relaxation in biological systems physical principles, methods, and applications

    CERN Document Server

    Feldman, Yuri

    2015-01-01

    This title covers the theoretical basis and practical aspects of the study of dielectric properties of biological systems, such as water, electrolyte and polyelectrolytes, solutions of biological macromolecules, cells suspensions and cellular systems.

  8. Single-molecule experiments in biological physics: methods and applications.

    Science.gov (United States)

    Ritort, F

    2006-08-16

    I review single-molecule experiments (SMEs) in biological physics. Recent technological developments have provided the tools to design and build scientific instruments of high enough sensitivity and precision to manipulate and visualize individual molecules and measure microscopic forces. Using SMEs it is possible to manipulate molecules one at a time and measure distributions describing molecular properties, characterize the kinetics of biomolecular reactions and detect molecular intermediates. SMEs provide additional information about thermodynamics and kinetics of biomolecular processes. This complements information obtained in traditional bulk assays. In SMEs it is also possible to measure small energies and detect large Brownian deviations in biomolecular reactions, thereby offering new methods and systems to scrutinize the basic foundations of statistical mechanics. This review is written at a very introductory level, emphasizing the importance of SMEs to scientists interested in knowing the common playground of ideas and the interdisciplinary topics accessible by these techniques. The review discusses SMEs from an experimental perspective, first exposing the most common experimental methodologies and later presenting various molecular systems where such techniques have been applied. I briefly discuss experimental techniques such as atomic-force microscopy (AFM), laser optical tweezers (LOTs), magnetic tweezers (MTs), biomembrane force probes (BFPs) and single-molecule fluorescence (SMF). I then present several applications of SME to the study of nucleic acids (DNA, RNA and DNA condensation) and proteins (protein-protein interactions, protein folding and molecular motors). Finally, I discuss applications of SMEs to the study of the nonequilibrium thermodynamics of small systems and the experimental verification of fluctuation theorems. I conclude with a discussion of open questions and future perspectives.

  9. Application of biological dural graft made by meninges from porkers

    Institute of Scientific and Technical Information of China (English)

    Peng Liu; Shengping Huang; Songtao Qi

    2007-01-01

    BACKGROUND: Presently, over 40 kinds of dural grafts have been successively used in clinic. Among them, lyophilized human dura mater with good histocompatibility and less complications is applied most widely. But there are a few reports on cases of infected spongiform encephalopathy following application of lyodura. More ideal repair materials deserve to be further investigated.OBJECTIVE: To investigate the efficiency and safety of biological dural graft made by meninges from porkers to repair meningeal injury.DESIGN: A self-control observation.SETTING: Wuhan General Hospital of Guangzhou Military Area Command of Chinese PLA.MATERIALS: Sixteen New Zealand Rabbits, of either gender, weighing from 2 to 3 kg, of clean grade Ⅱ,with the age of 0.5 - 1 year, were involved in this experiment. The involved rabbits were provided by the Animal Experimental Center of the First Military Medical University of Chinese PLA. Biological surgical patch (dural graft) was developed by Guangdong Guanhao Biotechnological Co.,Ltd. It was processed by using meninges from porkers by tissue engineering technology.METHODS: This experiment was carried out in the Experimental Center of the 157 Hospital of Chinese PLA between December 2003 and June 2004. ① The experimental rabbits were anesthetized. Dura mater was exposed from two sides ofpostmedial line of coronal suture. A rectangular dura mater about 8 mm × 8mm in size was cut off. Then a biological surgical patch (dural graft) was sheared into insert with 8 mm diameter and sutured. The left dura mater was untouched and used as control. Scalp was sutured, and postoperative wound healing and recovery were observed. ②The anesthetized rabbits were sacrificed at postoperative 3, 14, 30 and 90 days, 4 rabbits once. The whole head was cut off, and its scalp was removed.Afterwards, the head was fixed by formalin. Tissues in operative site were obtained, performed routine paraffin embedding, sliced and conducted HE staining, finally, the

  10. Development and application of biological technologies in fish genetic breeding.

    Science.gov (United States)

    Xu, Kang; Duan, Wei; Xiao, Jun; Tao, Min; Zhang, Chun; Liu, Yun; Liu, ShaoJun

    2015-02-01

    Fish genetic breeding is a process that remolds heritable traits to obtain neotype and improved varieties. For the purpose of genetic improvement, researchers can select for desirable genetic traits, integrate a suite of traits from different donors, or alter the innate genetic traits of a species. These improved varieties have, in many cases, facilitated the development of the aquaculture industry by lowering costs and increasing both quality and yield. In this review, we present the pertinent literatures and summarize the biological bases and application of selection breeding technologies (containing traditional selective breeding, molecular marker-assisted breeding, genome-wide selective breeding and breeding by controlling single-sex groups), integration breeding technologies (containing cross breeding, nuclear transplantation, germline stem cells and germ cells transplantation, artificial gynogenesis, artificial androgenesis and polyploid breeding) and modification breeding technologies (represented by transgenic breeding) in fish genetic breeding. Additionally, we discuss the progress our laboratory has made in the field of chromosomal ploidy breeding of fish, including distant hybridization, gynogenesis, and androgenesis. Finally, we systematically summarize the research status and known problems associated with each technology.

  11. The new biology of cancer: future clinical applications.

    Science.gov (United States)

    Yarbro, J W

    1989-06-01

    Our recognition that cancer evolves by clonal selection and that the basis for this selection is the growth advantage provided by the inappropriate expression or suppression of genes present in all normal cells allows us to anticipate that the pathways of this evolutionary process may be discovered. If a finite number of such pathways exist with some commonality between various tumors, there is the possibility to anticipate the mechanisms that cell clones might use for both carcinogenesis and tumor progression and to deal with evolving clones before their survival potential becomes overwhelming. As our biochemical understanding increases we may develop the capability to do the following: to identify individuals who have inherited or acquired defective cancer suppressor genes; to identify precursor lesions more exactly; to characterize the degree of progression of a newly diagnosed tumor (has the metastatic phenotype evolved?); to develop antibodies against cell membrane proteins necessary to tumor progression; to produce immune probes that carry cytotoxins or isotopes to specific cell populations; and to take advantage of cell signals to modify tumor growth. The potential for application of the New Biology to cancer medicine seems endless.

  12. 78 FR 78796 - Supplemental Applications Proposing Labeling Changes for Approved Drugs and Biological Products...

    Science.gov (United States)

    2013-12-27

    ... Applications Proposing Labeling Changes for Approved Drugs and Biological Products; Correction and Extension of... holders of an approved drug or biological product to change the product labeling to reflect certain types... biological product to change the product labeling to reflect certain types of newly acquired information...

  13. Zsyntax: a formal language for molecular biology with projected applications in text mining and biological prediction.

    Directory of Open Access Journals (Sweden)

    Giovanni Boniolo

    Full Text Available We propose a formal language that allows for transposing biological information precisely and rigorously into machine-readable information. This language, which we call Zsyntax (where Z stands for the Greek word zetaomegaeta, life, is grounded on a particular type of non-classical logic, and it can be used to write algorithms and computer programs. We present it as a first step towards a comprehensive formal language for molecular biology in which any biological process can be written and analyzed as a sort of logical "deduction". Moreover, we illustrate the potential value of this language, both in the field of text mining and in that of biological prediction.

  14. 9 CFR 114.2 - Products not prepared under license.

    Science.gov (United States)

    2010-01-01

    ... Biological Product License: (i) When a culture of microorganisms, isolated from a herd in a State, is... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Products not prepared under license. 114.2 Section 114.2 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE,...

  15. [Application of microelectronics CAD tools to synthetic biology].

    Science.gov (United States)

    Madec, Morgan; Haiech, Jacques; Rosati, Élise; Rezgui, Abir; Gendrault, Yves; Lallement, Christophe

    2017-02-01

    Synthetic biology is an emerging science that aims to create new biological functions that do not exist in nature, based on the knowledge acquired in life science over the last century. Since the beginning of this century, several projects in synthetic biology have emerged. The complexity of the developed artificial bio-functions is relatively low so that empirical design methods could be used for the design process. Nevertheless, with the increasing complexity of biological circuits, this is no longer the case and a large number of computer aided design softwares have been developed in the past few years. These tools include languages for the behavioral description and the mathematical modelling of biological systems, simulators at different levels of abstraction, libraries of biological devices and circuit design automation algorithms. All of these tools already exist in other fields of engineering sciences, particularly in microelectronics. This is the approach that is put forward in this paper.

  16. 78 FR 29393 - University of Missouri-Columbia Facility Operating License No. R-103

    Science.gov (United States)

    2013-05-20

    ... COMMISSION University of Missouri--Columbia Facility Operating License No. R-103 AGENCY: Nuclear Regulatory...) is considering an application for the renewal of Facility Operating License No. R-103 (``Application... application for the renewal of Facility Operating License No. R-103, which, currently authorizes the...

  17. Optical Properties and Biological Applications of Electromagnetically Coupled Metal Nanoparticles

    Science.gov (United States)

    Sheikholeslami, Sassan Nathan

    The optical properties of metallic particles change dramatically as the size shrinks to the nanoscale. The familiar mirror-like sheen of bulk metals is replaced by the bright, sharp, colorful plasmonic resonances of nanoparticles. The resonances of plasmonic metal nanoparticles are highly tunable throughout the visible spectrum, depending on the size, shape, local dielectric environment, and proximity to other optical resonances. Fundamental and applied research in the nanoscience community in the past few decades has sought to understand and exploit these phenomena for biological applications. In this work, discrete nanoparticle assemblies were produced through biomolecular interactions and studied at the single particle level with darkfield spectroscopy. Pairs of gold nanoparticles tethered by DNA were utilized as molecular rulers to study the dynamics of DNA bending by the restriction enzyme EcoRV. These results substantiated that nanoparticle rulers, deemed "plasmon rulers", could measure the dynamics of single biomolecules with high throughput, long lifetime, and high temporal resolution. To extend these concepts for live cell studies, a plasmon ruler comprised of peptide-linked gold nanoparticle satellites around a core particle was synthesized and utilized to optically follow cell signaling pathways in vivo at the single molecule level. The signal provided by these plasmon rulers allowed continuous observation of caspase-3 activation at the single molecule level in living cells for over 2 hours, unambiguously identifying early stage activation of caspase-3 in apoptotic cells. In the last section of this dissertation, an experimental and theoretical study of electomagnetic coupling in asymmetric metal nanoparticle dimers is presented. A "heterodimer" composed of a silver particle and a gold particle is observed to have a novel coupling between a plasmon mode (free electron oscillations) and an inter-band absorption process (bound electron transitions). The

  18. Multifunctional surfaces with discrete functionalized regions for biological applications.

    Science.gov (United States)

    Ghosh, Moniraj; Alves, Christina; Tong, Ziqiu; Tettey, Kwadwo; Konstantopoulos, Konstantinos; Stebe, Kathleen J

    2008-08-05

    In this paper we describe a method for creating multifunctional glass surfaces presenting discrete patches of different proteins on an inert PEG-functionalized background. Microcontact printing is used to stamp the substrate with octadecyltrichlorosilane to define the active regions. The substrate is then back-filled with PEG-silane {[[2-methoxypoly(ethyleneoxy)]propyl]trimethoxysilane} to define passive regions. A microfluidics device is subsequently affixed to the substrate to deliver proteins to the active regions, with as many channels as there are proteins to be patterned. Examples of trifunctional surfaces are given which present three terminating functional groups, i.e., protein 1, protein 2, and PEG. These surfaces should be broadly useful in biological studies, as patch size is well established to influence cell viability, growth, and differentiation. Three examples of cellular interactions with the surfaces are demonstrated, including the capture of cells from a single cell suspension, the selective sorting of cells from a mixed suspension, and the adhesion of cells to ligand micropatches at critical shear stresses. Within these examples, we demonstrate that the patterned immobilized proteins are active, as they retain their ability to interact with either antibodies in solution or receptors presented by cells. When appropriate (e.g., for E-selectin), proteins are patterned in their physiological orientations using a sandwich immobilization technique, which is readily accommodated within our method. The protein surface densities are highly reproducible in the patches, as supported by fluorescence intensity measurements. Potential applications include biosensors based on the interaction of cells or of marker proteins with protein patches, fundamental studies of cell adhesion as a function of patch size and shear stress, and studies of cell differentiation as a function of surface cues.

  19. Fluorescence correlation spectroscopy: Statistical analysis and biological applications

    Science.gov (United States)

    Saffarian, Saveez

    2002-01-01

    The experimental design and realization of an apparatus which can be used both for single molecule fluorescence detection and also fluorescence correlation and cross correlation spectroscopy is presented. A thorough statistical analysis of the fluorescence correlation functions including the analysis of bias and errors based on analytical derivations has been carried out. Using the methods developed here, the mechanism of binding and cleavage site recognition of matrix metalloproteinases (MMP) for their substrates has been studied. We demonstrate that two of the MMP family members, Collagenase (MMP-1) and Gelatinase A (MMP-2) exhibit diffusion along their substrates, the importance of this diffusion process and its biological implications are discussed. We show through truncation mutants that the hemopexin domain of the MMP-2 plays and important role in the substrate diffusion of this enzyme. Single molecule diffusion of the collagenase MMP-1 has been observed on collagen fibrils and shown to be biased. The discovered biased diffusion would make the MMP-1 molecule an active motor, thus making it the first active motor that is not coupled to ATP hydrolysis. The possible sources of energy for this enzyme and their implications are discussed. We propose that a possible source of energy for the enzyme can be in the rearrangement of the structure of collagen fibrils. In a separate application, using the methods developed here, we have observed an intermediate in the intestinal fatty acid binding protein folding process through the changes in its hydrodynamic radius also the fluctuations in the structure of the IFABP in solution were measured using FCS.

  20. Molecular biology techniques and applications for ocean sensing

    Directory of Open Access Journals (Sweden)

    J. P. Zehr

    2008-11-01

    Full Text Available The study of marine microorganisms using molecular biological techniques is now widespread in the ocean sciences. These techniques target nucleic acids which record the evolutionary history of microbes, and encode for processes which are active in the ocean today. Here we review some of the most commonly used molecular biological techniques. Molecular biological techniques permit study of the abundance, distribution, diversity, and physiology of microorganisms in situ. These techniques include the polymerase chain reaction (PCR and reverse-transcriptase PCR, quantitative PCR, whole assemblage "fingerprinting" approaches (based on nucleic acid sequence or length heterogeneity, oligonucleotide microarrays, and high-throughput shotgun sequencing of whole genomes and gene transcripts, which can be used to answer biological, ecological, evolutionary and biogeochemical questions in the ocean sciences. Moreover, molecular biological approaches may be deployed on ocean sensor platforms and hold promise for tracking of organisms or processes of interest in near-real time.

  1. Radioactive indicators in biology and their medical applications; Indicateurs radioactifs en biologie et leurs applications medicales

    Energy Technology Data Exchange (ETDEWEB)

    Morel, F

    1950-12-01

    This talk was given at the society of promotion of the national industry on December 1, 1949. The report is a pre-print of an article published in L'Industrie Nationale no.2 (1950). It presents the state-of-the-art of the use of artificial radioisotopes in biology and medicine: 1 - definitions (isotopes, decay, radiation emission); 2 - modalities of the use of radioisotopes in biology: basic instrumentation (Geiger-Mueller counter, counting techniques, photography, auto-histo-radiography); 3 - applications in physiology (digestive absorption, excretion, vascular exchanges, tracer techniques) and biochemistry (metabolism, thyroxine synthesis and fixation indicators, tracer techniques for drugs); 4 - radiotherapy, internal and external irradiation. (J.S.)

  2. 9th International Conference on Practical Applications of Computational Biology and Bioinformatics

    CERN Document Server

    Rocha, Miguel; Fdez-Riverola, Florentino; Paz, Juan

    2015-01-01

    This proceedings presents recent practical applications of Computational Biology and  Bioinformatics. It contains the proceedings of the 9th International Conference on Practical Applications of Computational Biology & Bioinformatics held at University of Salamanca, Spain, at June 3rd-5th, 2015. The International Conference on Practical Applications of Computational Biology & Bioinformatics (PACBB) is an annual international meeting dedicated to emerging and challenging applied research in Bioinformatics and Computational Biology. Biological and biomedical research are increasingly driven by experimental techniques that challenge our ability to analyse, process and extract meaningful knowledge from the underlying data. The impressive capabilities of next generation sequencing technologies, together with novel and ever evolving distinct types of omics data technologies, have put an increasingly complex set of challenges for the growing fields of Bioinformatics and Computational Biology. The analysis o...

  3. 9 CFR 102.1 - Licenses issued by the Administrator.

    Science.gov (United States)

    2010-01-01

    ... OF AGRICULTURE VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS LICENSES FOR... biological products under the Virus-Serum-Toxin Act shall hold an unexpired and unrevoked U.S. Veterinary... CFR parts 103 or 106 of this subchapter....

  4. 75 FR 39667 - Availability for Non-Exclusive or Partially Exclusive Licensing of a U.S. Patent Application

    Science.gov (United States)

    2010-07-12

    ... for preparing multivalent vaccines, recombinant DNA expression ] products and more particularly to..., Dr. Paul Mele, Office of Research and Technology Applications (ORTA), (301) 619- 6664, both...

  5. Scalable pattern recognition algorithms applications in computational biology and bioinformatics

    CERN Document Server

    Maji, Pradipta

    2014-01-01

    Reviews the development of scalable pattern recognition algorithms for computational biology and bioinformatics Includes numerous examples and experimental results to support the theoretical concepts described Concludes each chapter with directions for future research and a comprehensive bibliography

  6. Molecular biology techniques and applications for ocean sensing

    Directory of Open Access Journals (Sweden)

    J. P. Zehr

    2009-05-01

    Full Text Available The study of marine microorganisms using molecular biological techniques is now widespread in the ocean sciences. These techniques target nucleic acids which record the evolutionary history of microbes, and encode for processes which are active in the ocean today. Molecular techniques can form the basis of remote instrumentation sensing technologies for marine microbial diversity and ecological function. Here we review some of the most commonly used molecular biological techniques. These techniques include the polymerase chain reaction (PCR and reverse-transcriptase PCR, quantitative PCR, whole assemblage "fingerprinting" approaches (based on nucleic acid sequence or length heterogeneity, oligonucleotide microarrays, and high-throughput shotgun sequencing of whole genomes and gene transcripts, which can be used to answer biological, ecological, evolutionary and biogeochemical questions in the ocean sciences. Moreover, molecular biological approaches may be deployed on ocean sensor platforms and hold promise for tracking of organisms or processes of interest in near-real time.

  7. Application of E-infinity theory to biology

    Energy Technology Data Exchange (ETDEWEB)

    He Jihuan [College of Science, Donghua University, 1882 Yan-an Xilu Road, Shanghai 200051 (China)] e-mail: jhhe@dhu.edu.cn

    2006-04-01

    Albert Einstein combined continuous space and time into his special relativity, El-Naschie discovered the transfinite discontinuity of space-time in his E-infinity theory where infinity of dimensions was created. We find a partner of both space-time and E-infinity in biology. In our theory, the number of cells in an organism endows an additional dimension in biology, leading to explanation of many complex phenomena.

  8. 78 FR 30914 - Grand River Dam Authority Notice of Application for Temporary Variance of License and Soliciting...

    Science.gov (United States)

    2013-05-23

    ... Federal Energy Regulatory Commission Grand River Dam Authority Notice of Application for Temporary...: March 20, 2013, and supplemented on May 3, 2013, and May 16, 2013. d. Applicant: Grand River Dam..., Grand River Dam Authority, P.O. Box 409, Vinita, OK 74301; telephone: (918) 256-5545. i. FERC Contact:...

  9. 77 FR 27759 - City of Crystal Falls, MI; Notice of Application for Temporary Variance of License and Soliciting...

    Science.gov (United States)

    2012-05-11

    .... During the drawdown the City will conduct surveys for stranded mussels and fish. Additionally... Application: A copy of the application is available for inspection and reproduction at the Commission's Public... also available for inspection and reproduction at the address in item (h) above. m....

  10. 78 FR 57375 - Toutant Hydro Power, Inc.; Energy System, LLC.; Notice of Application for Transfer of License...

    Science.gov (United States)

    2013-09-18

    ... Power, Inc. (transferor) and Energy System, LLC (transferee) filed an application for transfer of... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Toutant Hydro Power, Inc.; Energy System, LLC.; Notice of Application...

  11. Biologic

    CERN Document Server

    Kauffman, L H

    2002-01-01

    In this paper we explore the boundary between biology and the study of formal systems (logic). In the end, we arrive at a summary formalism, a chapter in "boundary mathematics" where there are not only containers but also extainers ><, entities open to interaction and distinguishing the space that they are not. The boundary algebra of containers and extainers is to biologic what boolean algebra is to classical logic. We show how this formalism encompasses significant parts of the logic of DNA replication, the Dirac formalism for quantum mechanics, formalisms for protein folding and the basic structure of the Temperley Lieb algebra at the foundations of topological invariants of knots and links.

  12. Applications of aerospace technology in biology and medicine

    Science.gov (United States)

    Wooten, F. T.

    1972-01-01

    The results are presented of the medically related activities of the NASA Application Team Program at the Research Triangle Institute. The accomplishments of the Research Triangle Institute Application Team during the reporting period are as follows: The team has identified 44 new problems for investigation, has accomplished 8 technology applications and 8 potential technology applications, has closed 88 old problems, and reactivated 3 old problems, and on August 31, 1972, has a total of 57 problems under active investigation.

  13. Biological Membrane Ion Channels Dynamics, Structure, and Applications

    CERN Document Server

    Chung, Shin-Ho; Krishnamurthy, Vikram

    2007-01-01

    Ion channels are biological nanotubes that are formed by membrane proteins. Because ion channels regulate all electrical activities in living cells, understanding their mechanisms at a molecular level is a fundamental problem in biology. This book deals with recent breakthroughs in ion-channel research that have been brought about by the combined effort of experimental biophysicists and computational physicists, who together are beginning to unravel the story of these exquisitely designed biomolecules. With chapters by leading experts, the book is aimed at researchers in nanodevices and biosensors, as well as advanced undergraduate and graduate students in biology and the physical sciences. Key Features Presents the latest information on the molecular mechanisms of ion permeation through membrane ion channels Uses schematic diagrams to illustrate important concepts in biophysics Written by leading researchers in the area of ion channel investigations

  14. Synthetic Biology and Human Health: Potential Applications for Spaceflight

    Science.gov (United States)

    Karouia, Fathi; Carr, Christopher; Cai, Yizhi; Chen, Y.; Grenon, Marlene; Larios-Sanz, Maia; Jones, Jeffrey A.; Santos, Orlando

    2011-01-01

    Human space travelers experience a unique environment that affects homeostasis and physiologic adaptation. Spaceflight-related changes have been reported in the musculo-skeletal, cardiovascular, neurovestibular, endocrine, and immune systems. The spacecraft environment further subjects the traveler to noise and gravitational forces, as well as airborne chemical, microbiological contaminants, and radiation exposure. As humans prepare for longer duration missions effective countermeasures must be developed, verified, and implemented to ensure mission success. Over the past ten years, synthetic biology has opened new avenues for research and development in areas such as biological control, biomaterials, sustainable energy production, bioremediation, and biomedical therapies. The latter in particular is of great interest to the implementation of long-duration human spaceflight capabilities. This article discusses the effects of spaceflight on humans, and reviews current capabilities and potential needs associated with the health of the astronauts where synthetic biology could play an important role in the pursuit of space exploration.

  15. Application of XML database technology to biological pathway datasets.

    Science.gov (United States)

    Jiang, Keyuan; Nash, Christopher

    2006-01-01

    The study of biological systems has accumulated a significant amount of biological pathway data, which is evident through the continued growth in both the number of databases and amount of data available. The development of BioPAX standard leads to the increased availability of biological pathway datasets through the use of a special XML format, but the lack of standard storage mechanism makes the querying and aggregation of BioPAX compliant data challenging. To address this shortcoming, we have developed a storage mechanism leveraging the existing XML technologies: the XML database and XQuery. The goal of our project is to provide a generic and centralized store with efficient queries for the needs of biomedical research. A SOAP-based Web service and direct HTTP request methods have also developed to facilitate public consumption of the datasets online.

  16. 76 FR 27062 - Biologics Price Competition and Innovation Act of 2009; Options for a User Fee Program for...

    Science.gov (United States)

    2011-05-10

    ...; Options for a User Fee Program for Biosimilar and Interchangeable Biological Product Applications for... this document to request comments relating to the development of a user fee program for biosimilar and... similar (biosimilar) to, or interchangeable with, an FDA-licensed reference biological product....

  17. 75 FR 54343 - Center for Biologics Evaluation and Research eSubmitter Pilot Evaluation Program for Blood...

    Science.gov (United States)

    2010-09-07

    ...The Food and Drug Administration (FDA), Center for Biologics Evaluation and Research (CBER) is announcing an invitation to participate in a pilot evaluation program for CBER's eSubmitter Program (eSubmitter). CBER's eSubmitter has been customized as an automated biologics license application (BLA) and BLA supplement (BLS) submission system for blood and blood components. Participation in the......

  18. Application of random matrix theory to biological networks

    Energy Technology Data Exchange (ETDEWEB)

    Luo Feng [Department of Computer Science, Clemson University, 100 McAdams Hall, Clemson, SC 29634 (United States); Department of Pathology, U.T. Southwestern Medical Center, 5323 Harry Hines Blvd. Dallas, TX 75390-9072 (United States); Zhong Jianxin [Department of Physics, Xiangtan University, Hunan 411105 (China) and Oak Ridge National Laboratory, Oak Ridge, TN 37831 (United States)]. E-mail: zhongjn@ornl.gov; Yang Yunfeng [Oak Ridge National Laboratory, Oak Ridge, TN 37831 (United States); Scheuermann, Richard H. [Department of Pathology, U.T. Southwestern Medical Center, 5323 Harry Hines Blvd. Dallas, TX 75390-9072 (United States); Zhou Jizhong [Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019 (United States) and Oak Ridge National Laboratory, Oak Ridge, TN 37831 (United States)]. E-mail: zhouj@ornl.gov

    2006-09-25

    We show that spectral fluctuation of interaction matrices of a yeast protein-protein interaction network and a yeast metabolic network follows the description of the Gaussian orthogonal ensemble (GOE) of random matrix theory (RMT). Furthermore, we demonstrate that while the global biological networks evaluated belong to GOE, removal of interactions between constituents transitions the networks to systems of isolated modules described by the Poisson distribution. Our results indicate that although biological networks are very different from other complex systems at the molecular level, they display the same statistical properties at network scale. The transition point provides a new objective approach for the identification of functional modules.

  19. An analysis on the licensing trends of nuclear power plant and outstanding licensing issues

    Energy Technology Data Exchange (ETDEWEB)

    Han, Kyoo Sung; Nam, Seung Duk; Jang, Keun Sun; Yang, Joon Suk; Park, Tae Chul [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1993-08-01

    In this paper, nuclear licensing politics, procedures and regulatory trends of the national practices of both Korea and United States are reviewed and analyzed to begin with. The resolution process and regulatory status of severe accident issues, Unresolved Safety Issues(USIs) and Generic Safety Issues(GSIs) are also traced. Subsequently, the author suggests a scheme for resolving and preparing for anticipated licensing issues for Ulchin units 3 and 4, together with the establishment of outstanding licensing issues and regulatory criteria applicable to Ulchin units 3 and 4. 10 figs., 10 tabs., 22 refs. (Author).

  20. Geospatial Technology Applications and Infrastructure in the Biological Resources Division

    Science.gov (United States)

    D'Erchia, Frank; Getter, James; D'Erchia, Terry D.; Root, Ralph; Stitt, Susan; White, Barbara

    1998-01-01

    Executive Summary -- Automated spatial processing technology such as geographic information systems (GIS), telemetry, and satellite-based remote sensing are some of the more recent developments in the long history of geographic inquiry. For millennia, humankind has endeavored to map the Earth's surface and identify spatial relationships. But the precision with which we can locate geographic features has increased exponentially with satellite positioning systems. Remote sensing, GIS, thematic mapping, telemetry, and satellite positioning systems such as the Global Positioning System (GPS) are tools that greatly enhance the quality and rapidity of analysis of biological resources. These technologies allow researchers, planners, and managers to more quickly and accurately determine appropriate strategies and actions. Researchers and managers can view information from new and varying perspectives using GIS and remote sensing, and GPS receivers allow the researcher or manager to identify the exact location of interest. These geospatial technologies support the mission of the U.S. Geological Survey (USGS) Biological Resources Division (BRD) and the Strategic Science Plan (BRD 1996) by providing a cost-effective and efficient method for collection, analysis, and display of information. The BRD mission is 'to work with others to provide the scientific understanding and technologies needed to support the sound management and conservation of our Nation's biological resources.' A major responsibility of the BRD is to develop and employ advanced technologies needed to synthesize, analyze, and disseminate biological and ecological information. As the Strategic Science Plan (BRD 1996) states, 'fulfilling this mission depends on effectively balancing the immediate need for information to guide management of biological resources with the need for technical assistance and long-range, strategic information to understand and predict emerging patterns and trends in ecological systems

  1. Click chemistry mediated functionalization of vertical nanowires for biological applications

    DEFF Research Database (Denmark)

    Vutti, Surendra; Schoffelen, Sanne; Bolinsson, Jessica;

    2016-01-01

    is of general interest for biological studies. The attachment of a peptide substrate provided NW arrays for the detection of protease activity. In addition, green fluorescent protein was immobilized in a site-specific manner and recognized by antibody binding to demonstrate the proof-of-concept for the use...

  2. Human mesenchymal stromal cells : biological characterization and clinical application

    NARCIS (Netherlands)

    Bernardo, Maria Ester

    2010-01-01

    This thesis focuses on the characterization of the biological and functional properties of human mesenchymal stromal cells (MSCs), isolated from different tissue sources. The differentiation capacity of MSCs from fetal and adult tissues has been tested and compared. Umbilical cord blood (UCB) has be

  3. 78 FR 47800 - License Renewal Application for Byron Station, Units 1 and 2; Exelon Generation Company, LLC

    Science.gov (United States)

    2013-08-06

    ... authorized to develop and enforce relevant environmental standards; c. Affected State and local government agencies, including those authorized to develop and enforce relevant environmental standards; d. Any... Register on June 13, 2013 (78 FR 35646). A notice of acceptance for docketing of the application...

  4. 78 FR 46379 - Exelon Generation Company, LLC, License Renewal Application for Braidwood Station, Units 1 and 2

    Science.gov (United States)

    2013-07-31

    ... authorized to develop and enforce relevant environmental standards; c. Affected State and local government agencies, including those authorized to develop and enforce relevant environmental standards; d. Any... Register on June 13, 2013 (78 FR 35646). A notice of acceptance for docketing of the application...

  5. 77 FR 39675 - Applications for Licensing as a Non-Leveraged Rural Business Investment Company Under the Rural...

    Science.gov (United States)

    2012-07-05

    ... economic development and the creation of wealth and job opportunities in rural areas and among individuals...: (202) 720-1400. VIII. Nondiscrimination Statement USDA prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex,...

  6. 15 CFR Appendix 1 to Part 960 - Filing Instructions and Information To Be Included in the Licensing Application

    Science.gov (United States)

    2010-01-01

    ... subsidiaries, with foreign nation or person, including copies if available; (3) A copy of the charter or other... sensor (IFOV, in-track, cross-track); (6) On-board storage capacity; (7) Navigation capabilities—GPS... benefit purposes, such as the study of the changing global environment. B. If the applicant is...

  7. 77 FR 40142 - Applications for Exemption: Commercial Driver's License (CDL) and Hours-of-Service (HOS) of Drivers

    Science.gov (United States)

    2012-07-06

    ... May 21, 2012, and of Underwater Construction Corporation (Underwater) on May 29, 2012. On June 4, 2012....regulations.gov at any time, or to Room W12-140, DOT Building, 1200 New Jersey Ave. SE., Washington, DC... the case with the denials announced today. Applications for Exemption Rotel, Underwater,...

  8. 78 FR 21923 - City of Crystal Falls, MI; Notice of Application for Temporary Variance of License and Soliciting...

    Science.gov (United States)

    2013-04-12

    ... the City will conduct surveys for stranded mussels and fish. Additionally, recreational access will be... the application is available for inspection and reproduction at the Commission's Public Reference Room... also available for inspection and reproduction at the address in item (h) above. m....

  9. Allegheny County Dog Licenses

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — A list of dog license dates, dog breeds, and dog name by zip code. Currently this dataset does not include City of Pittsburgh dogs.

  10. Application of computational systems biology to explore environmental toxicity hazards

    DEFF Research Database (Denmark)

    Audouze, Karine Marie Laure; Grandjean, Philippe

    2011-01-01

    Background: Computer-based modeling is part of a new approach to predictive toxicology.Objectives: We investigated the usefulness of an integrated computational systems biology approach in a case study involving the isomers and metabolites of the pesticide dichlorodiphenyltrichloroethane (DDT......) to ascertain their possible links to relevant adverse effects.Methods: We extracted chemical-protein association networks for each DDT isomer and its metabolites using ChemProt, a disease chemical biology database that includes both binding and gene expression data, and we explored protein-protein interactions...... using a human interactome network. To identify associated dysfunctions and diseases, we integrated protein-disease annotations into the protein complexes using the Online Mendelian Inheritance in Man database and the Comparative Toxicogenomics Database.Results: We found 175 human proteins linked to p,p´-DDT...

  11. Neutron scattering applications in structural biology: now and the future

    Energy Technology Data Exchange (ETDEWEB)

    Trewhella, J. [Los Alamos National Lab., NM (United States)

    1996-05-01

    Neutrons have an important role to play in structural biology. Neutron crystallography, small-angle neutron scattering and inelastic neutron scattering techniques all contribute unique information on biomolecular structures. In particular, solution scattering techniques give critical information on the conformations and dispositions of the components of complex assemblies under a wide variety of relevant conditions. The power of these methods is demonstrated here by studies of protein/DNA complexes, and Ca{sup 2+}-binding proteins complexed with their regulatory targets. In addition, we demonstrate the utility of a new structural approach using neutron resonance scattering. The impact of biological neutron scattering to date has been constrained principally by the available fluxes at neutron sources and the true potential of these approaches will only be realized with the development of new more powerful neutron sources. (author)

  12. Biological applications of cryo-soft X-ray tomography.

    Science.gov (United States)

    Duke, E; Dent, K; Razi, M; Collinson, L M

    2014-08-01

    X-rays are used for imaging many different types of biological specimen, ranging from live organisms to the individual cells and proteins from which they are made. The level of detail achieved as a result of the imaging varies depending on both the sample and the technique used. One of the most recent technical developments in X-ray imaging is that of the soft X-ray microscope, designed to allow the internal structure of individual biological cells to be explored. With a field of view of ∼10-20 × ∼10-20 μm, a penetration depth of ∼10 μm and a resolution of ∼40 nm(3), the soft X-ray microscope neatly fits between the imaging capabilities of light and electron microscopes.

  13. Applications of membrane computing in systems and synthetic biology

    CERN Document Server

    Gheorghe, Marian; Pérez-Jiménez, Mario

    2014-01-01

    Membrane Computing was introduced as a computational paradigm in Natural Computing. The models introduced, called Membrane (or P) Systems, provide a coherent platform to describe and study living cells as computational systems. Membrane Systems have been investigated for their computational aspects and employed to model problems in other fields, like: Computer Science, Linguistics, Biology, Economy, Computer Graphics, Robotics, etc. Their inherent parallelism, heterogeneity and intrinsic versatility allow them to model a broad range of processes and phenomena, being also an efficient means to solve and analyze problems in a novel way. Membrane Computing has been used to model biological systems, becoming with time a thorough modeling paradigm comparable, in its modeling and predicting capabilities, to more established models in this area. This book is the result of the need to collect, in an organic way, different facets of this paradigm. The chapters of this book, together with the web pages accompanying th...

  14. Optical sensors and their applications for probing biological systems

    DEFF Research Database (Denmark)

    Palanco, Marta Espina

    and mammalian cells. First, we performed Surface Enhanced Raman Spectroscopy (SERS) studies on intact plant materials via using silver plasmonic nanostructures. Our studies showed strong Raman signals which resemble to the presence of typical constituents such as carbohydrates, proteins and lipids of different......There is a great interest in exploring and developing new optical sensitive methodologies for probing complex biological systems. In this project we developed non-invasive and sensitive biosensor strategies for studying physiologically relevant chemical and physical properties of plant...... biological sample to provide a SERS-template where silver nanoparticles can grow, thus providing a new insight into SERS-based sensors for chemically sensing in-situ plant constituents. Optical manipulation techniques have been used to investigate mechanical properties of soft membrane cells, i.e. mammalian...

  15. Fish gelatin thin film standards for biological application of PIXE

    Science.gov (United States)

    Manuel, Jack E.; Rout, Bibhudutta; Szilasi, Szabolcs Z.; Bohara, Gyanendra; Deaton, James; Luyombya, Henry; Briski, Karen P.; Glass, Gary A.

    2014-08-01

    There exists a critical need to understand the flow and accumulation of metallic ions, both naturally occurring and those introduced to biological systems. In this paper the results of fabricating thin film elemental biological standards containing nearly any combination of trace elements in a protein matrix are presented. Because it is capable of high elemental sensitivity, particle induced X-ray emission spectrometry (PIXE) is an excellent candidate for in situ analysis of biological tissues. Additionally, the utilization of microbeam PIXE allows the determination of elemental concentrations in and around biological cells. However, obtaining elemental reference standards with the same matrix constituents as brain tissue is difficult. An excellent choice for simulating brain-like tissue is Norland® photoengraving glue which is derived from fish skin. Fish glue is water soluble, liquid at room temperature, and resistant to dilute acid. It can also be formed into a thin membrane which dries into a durable, self-supporting film. Elements of interest are introduced to the fish glue in precise volumetric additions of well quantified atomic absorption standard solutions. In this study GeoPIXE analysis package is used to quantify elements intrinsic to the fish glue as well as trace amounts of manganese added to the sample. Elastic (non-Rutherford) backscattered spectroscopy (EBS) and the 1.734 MeV proton-on-carbon 12C(p,p)12C resonance is used for a normalization scheme of the PIXE spectra to account for any discrepancies in X-ray production arising from thickness variation of the prepared standards. It is demonstrated that greater additions of the atomic absorption standard cause a viscosity reduction of the liquid fish glue resulting in thinner films but the film thickness can be monitored by using simultaneous PIXE and EBS proton data acquisition.

  16. BioInt: an integrative biological object-oriented application framework and interpreter.

    Science.gov (United States)

    Desai, Sanket; Burra, Prasad

    2015-01-01

    BioInt, a biological programming application framework and interpreter, is an attempt to equip the researchers with seamless integration, efficient extraction and effortless analysis of the data from various biological databases and algorithms. Based on the type of biological data, algorithms and related functionalities, a biology-specific framework was developed which has nine modules. The modules are a compilation of numerous reusable BioADTs. This software ecosystem containing more than 450 biological objects underneath the interpreter makes it flexible, integrative and comprehensive. Similar to Python, BioInt eliminates the compilation and linking steps cutting the time significantly. The researcher can write the scripts using available BioADTs (following C++ syntax) and execute them interactively or use as a command line application. It has features that enable automation, extension of the framework with new/external BioADTs/libraries and deployment of complex work flows.

  17. 10 CFR 50.51 - Continuation of license.

    Science.gov (United States)

    2010-01-01

    ... Commission upon the expiration of the period. Renewal of operating licenses for nuclear power plants is... NUCLEAR REGULATORY COMMISSION DOMESTIC LICENSING OF PRODUCTION AND UTILIZATION FACILITIES Issuance... continue to maintain the facility, including, where applicable, the storage, control and maintenance of...

  18. 76 FR 45838 - Government-Owned Inventions; Availability for Licensing

    Science.gov (United States)

    2011-08-01

    ... federally-funded research and development. Foreign patent applications are filed on selected inventions to extend market coverage for companies and may also be available for licensing. ADDRESSES: Licensing... targeting of treatment Targeted treatment decreases non-specific killing of healthy, essential...

  19. 76 FR 49777 - Government-Owned Inventions; Availability for Licensing

    Science.gov (United States)

    2011-08-11

    ... federally-funded research and development. Foreign patent applications are filed on selected inventions to extend market coverage for companies and may also be available for licensing. ADDRESSES: Licensing... chemotherapy. The researchers have developed a quantitative antibody-based testing method for...

  20. 77 FR 65673 - Prospective Grant of Exclusive Patent License

    Science.gov (United States)

    2012-10-30

    ... National Institute of Standards and Technology Prospective Grant of Exclusive Patent License AGENCY... patent license. SUMMARY: This is a notice in accordance with 35 U.S.C. 209(e) and 37 CFR 404.7(a)(1)(i... and commonwealths, to NIST's interest in the invention embodied in U.S. Patent Application No....

  1. Thermophilic and alkaliphilic Actinobacteria: biology and potential applications.

    Science.gov (United States)

    Shivlata, L; Satyanarayana, Tulasi

    2015-01-01

    Microbes belonging to the phylum Actinobacteria are prolific sources of antibiotics, clinically useful bioactive compounds and industrially important enzymes. The focus of the current review is on the diversity and potential applications of thermophilic and alkaliphilic actinobacteria, which are highly diverse in their taxonomy and morphology with a variety of adaptations for surviving and thriving in hostile environments. The specific metabolic pathways in these actinobacteria are activated for elaborating pharmaceutically, agriculturally, and biotechnologically relevant biomolecules/bioactive compounds, which find multifarious applications.

  2. Stochastic narrow escape in molecular and cellular biology analysis and applications

    CERN Document Server

    Holcman, David

    2015-01-01

    This book covers recent developments in the non-standard asymptotics of the mathematical narrow escape problem in stochastic theory, as well as applications of the narrow escape problem in cell biology. The first part of the book concentrates on mathematical methods, including advanced asymptotic methods in partial equations, and is aimed primarily at applied mathematicians and theoretical physicists who are interested in biological applications. The second part of the book is intended for computational biologists, theoretical chemists, biochemists, biophysicists, and physiologists. It includes a summary of output formulas from the mathematical portion of the book and concentrates on their applications in modeling specific problems in theoretical molecular and cellular biology. Critical biological processes, such as synaptic plasticity and transmission, activation of genes by transcription factors, or double-strained DNA break repair, are controlled by diffusion in structures that have both large and small sp...

  3. International Conference on Recent Advances in Mathematical Biology, Analysis and Applications

    CERN Document Server

    Saleem, M; Srivastava, H; Khan, Mumtaz; Merajuddin, M

    2016-01-01

    The book contains recent developments and contemporary research in mathematical analysis and in its application to problems arising from the biological and physical sciences. The book is of interest to readers who wish to learn of new research in such topics as linear and nonlinear analysis, mathematical biology and ecology, dynamical systems, graph theory, variational analysis and inequalities, functional analysis, differential and difference equations, partial differential equations, approximation theory, and chaos. All papers were prepared by participants at the International Conference on Recent Advances in Mathematical Biology, Analysis and Applications (ICMBAA-2015) held during 4–6 June 2015 in Aligarh, India. A focal theme of the conference was the application of mathematics to the biological sciences and on current research in areas of theoretical mathematical analysis that can be used as sophisticated tools for the study of scientific problems. The conference provided researchers, academicians and ...

  4. [Application of synthetic biology to sustainable utilization of Chinese materia medica resources].

    Science.gov (United States)

    Huang, Lu-Qi; Gao, Wei; Zhou, Yong-Jin

    2014-01-01

    Bioactive natural products are the material bases of Chinese materia medica resources. With successful applications of synthetic biology strategies to the researches and productions of taxol, artemisinin and tanshinone, etc, the potential ability of synthetic biology in the sustainable utilization of Chinese materia medica resources has been attracted by many researchers. This paper reviews the development of synthetic biology, the opportunities of sustainable utilization of Chinese materia medica resources, and the progress of synthetic biology applied to the researches of bioactive natural products. Furthermore, this paper also analyzes how to apply synthetic biology to sustainable utilization of Chinese materia medica resources and what the crucial factors are. Production of bioactive natural products with synthetic biology strategies will become a significant approach for the sustainable utilization of Chinese materia medica resources.

  5. Blueprints for green biotech: development and application of standards for plant synthetic biology.

    Science.gov (United States)

    Patron, Nicola J

    2016-06-15

    Synthetic biology aims to apply engineering principles to the design and modification of biological systems and to the construction of biological parts and devices. The ability to programme cells by providing new instructions written in DNA is a foundational technology of the field. Large-scale de novo DNA synthesis has accelerated synthetic biology by offering custom-made molecules at ever decreasing costs. However, for large fragments and for experiments in which libraries of DNA sequences are assembled in different combinations, assembly in the laboratory is still desirable. Biological assembly standards allow DNA parts, even those from multiple laboratories and experiments, to be assembled together using the same reagents and protocols. The adoption of such standards for plant synthetic biology has been cohesive for the plant science community, facilitating the application of genome editing technologies to plant systems and streamlining progress in large-scale, multi-laboratory bioengineering projects.

  6. Dynamic light scattering with applications to chemistry, biology, and physics

    CERN Document Server

    Berne, Bruce J

    2000-01-01

    Lasers play an increasingly important role in a variety of detection techniques, making inelastic light scattering a tool of growing value in the investigation of dynamic and structural problems in chemistry, biology, and physics. Until the initial publication of this work, however, no monograph treated the principles behind current developments in the field.This volume presents a comprehensive introduction to the principles underlying laser light scattering, focusing on the time dependence of fluctuations in fluid systems; it also serves as an introduction to the theory of time correlation f

  7. Shapeshifting photoswitchable azobenzene compounds and their biological applications

    Science.gov (United States)

    Peddie, Victoria; Heng, Sabrina; Mustafa, Sanam; Thomas, Jacob; Hutchinson, Mark R.; Abell, Andrew D.

    2016-11-01

    The photoisomerisation of azobenzenes between trans and cis results in well-defined changes in geometry and a considerable change of polarity. Thus, incorporating an azobenezene into a bioactive compound provides an opportunity to control biological activity, with ideally one configuration being active and the other inactive. This can allow the role of a specific biomolecule to be probed in its native environment by controlling activity both spatially and temporally using light. Incorporating such a photoswitchable moiety into the structure of a known GRK2 inhibitor can generate photoswitchable inhibitors, which can be used to reversibly regulate the activity of GRK2, and hence GPRCs.

  8. A BWR licensing experience in the USA

    Energy Technology Data Exchange (ETDEWEB)

    Powers, J.; Ogura, C. [Toshiba America Nuclear Energy, Charlotte, North Carolina (United States); Arai, K. [Toshiba Corporation, Yokohama, Kanagawa (Japan); Thomas, S.; Mookhoek, B., E-mail: jim.powers@toshiba.com [Nuclear Innovation North America, Lake Jackson, Texas (United States)

    2015-09-15

    The US-Advanced Boiling Water Reactor (A BWR), certified by the United States Nuclear Regulatory Commission (US NRC), is a third generation, evolutionary boiling water reactor design which is the reference for the South Texas Project Units 3 and 4 (STP3-4) Combined License Application (Cola). Nuclear Innovation North America (Nina) is the License Applicant for this new build project, and Toshiba is the selected primary technology contractor. The STP3-4 project has finished the US NRC technical review of the Cola through the final meeting of the Advisory Committee on Reactor Safeguards (ACRS), and the Final Safety Evaluation Report (FSER) is scheduled to be issued by the US NRC in the middle of 2015. The next steps are to support the Mandatory Hearing process, and voting by the NRC commissioners on the motion to grant the Combined License, which is scheduled beginning of 2016 according to US NRC schedule as of March 30, 2015. This paper summarizes the history and progress of the US-A BWR licensing, including the experiences of the Licensee, Nina, and Toshiba as the Epc team worked through the Code of Federal Regulations Title 10 (10-Cfr) Part 52 process, and provides some perspectives on how the related licensing material would also be of value within a 10-Cfr Part 50, two-step process to minimize schedule and financial risks which could arise from ongoing technical developments and regulatory reviews. (Author)

  9. Quantum Information Biology: From Information Interpretation of Quantum Mechanics to Applications in Molecular Biology and Cognitive Psychology

    Science.gov (United States)

    Asano, Masanari; Basieva, Irina; Khrennikov, Andrei; Ohya, Masanori; Tanaka, Yoshiharu; Yamato, Ichiro

    2015-10-01

    We discuss foundational issues of quantum information biology (QIB)—one of the most successful applications of the quantum formalism outside of physics. QIB provides a multi-scale model of information processing in bio-systems: from proteins and cells to cognitive and social systems. This theory has to be sharply distinguished from "traditional quantum biophysics". The latter is about quantum bio-physical processes, e.g., in cells or brains. QIB models the dynamics of information states of bio-systems. We argue that the information interpretation of quantum mechanics (its various forms were elaborated by Zeilinger and Brukner, Fuchs and Mermin, and D' Ariano) is the most natural interpretation of QIB. Biologically QIB is based on two principles: (a) adaptivity; (b) openness (bio-systems are fundamentally open). These principles are mathematically represented in the framework of a novel formalism— quantum adaptive dynamics which, in particular, contains the standard theory of open quantum systems.

  10. Compatibility analysis of 3D printer resin for biological applications

    KAUST Repository

    Sivashankar, Shilpa

    2016-08-30

    The salient features of microfluidics such as reduced cost, handling small sample and reagent volumes and less time required to fabricate the devices has inspired the present work. The incompatibility of three-dimensional printer resins in their native form and the method to improve their compatibility to many biological processes via surface modification are reported. The compatibility of the material to build microfluidic devices was evaluated in three different ways: (i) determining if the ultraviolet (UV) cured resin inhibits the polymerase chain reaction (PCR), i.e. testing devices for PCR compatibility; (ii) observing agglutination complex formed on the surface of the UV cured resin when anti-C-reactive protein (CRP) antibodies and CRP proteins were allowed to agglutinate; and (iii) by culturing human embryonic kidney cell line cells and testing for its attachment and viability. It is shown that only a few among four in its native form could be used for fabrication of microchannels and that had the least effect on biological molecules that could be used for PCR and protein interactions and cells, whereas the others were used after treating the surface. Importance in building lab-on-chip/micrototal analysis systems and organ-on-chip devices is found.

  11. Thermophilic and alkaliphilic Actinobacteria: Biology and potential applications

    Directory of Open Access Journals (Sweden)

    L eShivlata

    2015-09-01

    Full Text Available Microbes belonging to the phylum Actinobacteria are prolific sources of antibiotics, clinically useful bioactive compounds and industrially important enzymes. The focus of the current review is on the diversity and potential applications of thermophilic and alkaliphilic actinobacteria, which are highly diverse in their taxonomy and morphology with a variety of adaptations for surviving and thriving in hostile environments. The specific metabolic pathways in these actinobacteria are activated for elaborating pharmaceutically, agriculturally and biotechnologically relevant biomolecules/bioactive compounds, which find multifarious applications.

  12. Testing of Synthetic Biological Membranes for Forward Osmosis Applications

    Science.gov (United States)

    Parodi, Jurek; Mangado, Jaione Romero; Stefanson, Ofir; Flynn, Michael; Mancinelli, Rocco; Kawashima, Brian; Trieu, Serena; Brozell, Adrian; Rosenberg, Kevan

    2016-01-01

    Commercially available forward osmosis membranes have been extensively tested for human space flight wastewater treatment. Despite the improvements achieved in the last decades, there is still a challenge to produce reliable membranes with anti-fouling properties, chemical resistance, and high flux and selectivity. Synthetic biological membranes that mimic the ones present in nature, which underwent millions of years of evolution, represent a potential solution for further development and progress in membrane technology. Biomimetic forward osmosis membranes based on a polymeric support filter and coated with surfactant multilayers have been engineered to investigate how different manufacturing processes impact the performance and structure of the membrane. However, initial results of the first generation prototype membranes tests reveal a high scatter in the data, due to the current testing apparatus set up. The testing apparatus has been upgraded to improve data collection, reduce errors, and to allow higher control of the testing process.

  13. Field applications demonstrate capabilities of upflow biological reactors

    Energy Technology Data Exchange (ETDEWEB)

    Hohl, H.M. (Celgene Corp., Warren, NJ (United States))

    1994-05-01

    Biological solutions for treating hazardous and non-hazardous waste streams have existed for more than 100 years. Secondary treatment starting with the trickling filter evolved into activated sludge processes that today are used routinely to reduced organics in water streams. However, such treatment processes are limited to relatively narrow operating parameters. Temperature, pH and organic loading are only some of the operating parameters that must fall into narrow ranges to facilitate degradation. Upsets occur when parameters go outside these ranges. Two case studies are discussed where methylene chloride is successfully removed from industrial plant discharges by biodegradation. In another study, phenol and aromatics were effectively removed from industrial waste water by an in-process biotreatment system.

  14. Application of biological dosimetry in accidental radiation exposure

    Energy Technology Data Exchange (ETDEWEB)

    Nosal, M.; Batora, I.; Kolesar, D.; Stojkovic, J. (Komenskeho Univ., Bratislava (Czechoslovakia). Lekarska Fakulta); Gaal, P.; Sklovsky, A. (Krajska Hygienicka Stanica, Bratislava (Czechoslovakia)); Cizova, O. (Sexuologicka Ambulancia KUNZ, Bratislava (Czechoslovakia))

    1982-03-01

    The case is described of accidental irradiation of a male person with /sup 137/Cs of an activity of 24.71 GBq. The first estimate induced a reasonable suspicion that the absorbed dose could be very high and life-threatening. On the other hand the clinical picture, usual laboratory examinations, findings in the fluorescent blood count, the analysis of chromosomal count of lymphocytes in the peripheral blood, the spermiogram, and the negative post-irradiation porphyrinuria suggested that the absorbed dose could be much lower than the original estimate. The results of dosimetry obtained after the reconstruction of the accident by measuring on a phantom revealed that the actual dose was very close to that presumed from the results of biological dosimetry during the first days of examination of the patient.

  15. Inelastic scattering of neutrons and possible biological applications.

    Science.gov (United States)

    Egelstaff, P A

    1976-05-01

    The field of neutron inelastic scattering has probably been developed to the stage where it can begin to help the biologist. Because essentially no experimental data have been obtained, it is difficult either to draw conclusions or to make forecasts except on the basis of general hypotheses. It seems likely, however, that the next stage is up to biologists. After reviewing those biological problems in which molecular dynamics might play an important role, they should suggest specimens of interest which can give inelastic peaks with existing spectrometers operating with 5 to 10-A neutrons at angles greater than 5degrees and with resolutions of approximately 50 mueV. These specimens may involve molecules slightly smaller and more mobile than some biologists would like, but a successful outcome might lead to the development of spectrometers capable of working in a more satisfactory range. In this event the return may well prove rewarding to the biologists.

  16. Porous titanium for biomedical applications : development, characterization and biological evaluation

    NARCIS (Netherlands)

    Li, JiaPing

    2007-01-01

    Metallic biomaterials have so far shown the greatest potential to be the basis of implants for long-term load-bearing orthopedic and dental applications, owing to their excellent mechanical strength when compared to alternative biomaterials, such as polymers and ceramics. Particularly titanium and i

  17. Geospatial Technology Applications and Infrastructure in the Biological Resources Division.

    Science.gov (United States)

    1998-09-01

    Laguna Madre , Texas , most particularly to docu- ment the effects of light reduction by a persistent phytoplankton bloom. In another GPS application, the...information will be used to determine whether different physiological phenotypes vary in migration routes. Texas Cooperative Fish and Wildlife...Research Unit: The Texas Cooperative Fish and Wildlife Research Unit has developed methodologies that combine aerial videography, Landsat TM imagery

  18. Biclustering methods: biological relevance and application in gene expression analysis.

    Directory of Open Access Journals (Sweden)

    Ali Oghabian

    Full Text Available DNA microarray technologies are used extensively to profile the expression levels of thousands of genes under various conditions, yielding extremely large data-matrices. Thus, analyzing this information and extracting biologically relevant knowledge becomes a considerable challenge. A classical approach for tackling this challenge is to use clustering (also known as one-way clustering methods where genes (or respectively samples are grouped together based on the similarity of their expression profiles across the set of all samples (or respectively genes. An alternative approach is to develop biclustering methods to identify local patterns in the data. These methods extract subgroups of genes that are co-expressed across only a subset of samples and may feature important biological or medical implications. In this study we evaluate 13 biclustering and 2 clustering (k-means and hierarchical methods. We use several approaches to compare their performance on two real gene expression data sets. For this purpose we apply four evaluation measures in our analysis: (1 we examine how well the considered (biclustering methods differentiate various sample types; (2 we evaluate how well the groups of genes discovered by the (biclustering methods are annotated with similar Gene Ontology categories; (3 we evaluate the capability of the methods to differentiate genes that are known to be specific to the particular sample types we study and (4 we compare the running time of the algorithms. In the end, we conclude that as long as the samples are well defined and annotated, the contamination of the samples is limited, and the samples are well replicated, biclustering methods such as Plaid and SAMBA are useful for discovering relevant subsets of genes and samples.

  19. 78 FR 7818 - Request To Amend a License To Export Radioactive Waste

    Science.gov (United States)

    2013-02-04

    ... COMMISSION Request To Amend a License To Export Radioactive Waste Pursuant to 10 CFR 110.70 (b) ``Public...) has received the following request for an export license amendment. Copies of the request are... and Adjudications The information concerning this export license amendment application follows. ]...

  20. 47 CFR 13.201 - Qualifying for a commercial operator license or endorsement.

    Science.gov (United States)

    2010-10-01

    ... RADIO OPERATORS Examination System § 13.201 Qualifying for a commercial operator license or endorsement. (a) To be qualified to hold any commercial radio operator license, an applicant must have a... commercial radio operator license, other than the Restricted Radiotelephone Operator Permit and...

  1. 10 CFR 51.105a - Public hearings in proceedings for issuance of manufacturing licenses.

    Science.gov (United States)

    2010-01-01

    ... manufacturing licenses. 51.105a Section 51.105a Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) ENVIRONMENTAL... hearings in proceedings for issuance of manufacturing licenses. In addition to complying with applicable requirements of § 51.31(c), in a proceeding for the issuance of a manufacturing license, the presiding...

  2. Non-Power Reactor Operator Licensing Examiner Standards. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-06-01

    The Non-Power Reactor Operator Licensing Examiner Standards provide policy and guidance to NRC examiners and establish the procedures and practices for examining and licensing of applicants for NRC operator licenses pursuant to Part 55 of Title 10 of the Code of Federal Regulations (10 CFR 55). They are intended to assist NRC examiners and facility licensees to understand the examination process better and to provide for equitable and consistent administration of examinations to all applicants by NRC examiners. These standards are not a substitute for the operator licensing regulations and are subject to revision or other internal operator examination licensing policy changes. As appropriate, these standards will be revised periodically to accommodate comments and reflect new information or experience.

  3. Application of 19F MRI for in vivo detection of biological processes

    OpenAIRE

    Basse-Lüsebrink, Thomas Christian

    2013-01-01

    This thesis focuses on various aspects and techniques of 19F magnetic resonance (MR). The first chapters provide an overview of the basic physical properties, 19F MR and MR sequences related to this work. Chapter 5 focuses on the application of 19F MR to visualize biological processes in vivo using two different animal models. The dissimilar models underlined the wide applicability of 19F MR in preclinical research. A subsection of Chapter 6 shows the application of compressed sensing (CS) to...

  4. Applications of Discrete Molecular Dynamics in biology and medicine.

    Science.gov (United States)

    Proctor, Elizabeth A; Dokholyan, Nikolay V

    2016-04-01

    Discrete Molecular Dynamics (DMD) is a physics-based simulation method using discrete energetic potentials rather than traditional continuous potentials, allowing microsecond time scale simulations of biomolecular systems to be performed on personal computers rather than supercomputers or specialized hardware. With the ongoing explosion in processing power even in personal computers, applications of DMD have similarly multiplied. In the past two years, researchers have used DMD to model structures of disease-implicated protein folding intermediates, study assembly of protein complexes, predict protein-protein binding conformations, engineer rescue mutations in disease-causative protein mutants, design a protein conformational switch to control cell signaling, and describe the behavior of polymeric dispersants for environmental cleanup of oil spills, among other innovative applications.

  5. 77 FR 4887 - DOE Patent Licensing Regulations

    Science.gov (United States)

    2012-02-01

    ... Part 781 RIN 1990-AA41 DOE Patent Licensing Regulations AGENCY: Office of the General Counsel, Department of Energy. ACTION: Final rule. SUMMARY: The Department of Energy (DOE) is amending its patent... patents or patent applications vested in the United States of America, as represented by or in the...

  6. Development of Droplet-Based Microfluidics for Synthetic Biology Applications

    OpenAIRE

    Janiesch, Jan-Willi

    2015-01-01

    Microfluidics combines principles of science and technology, and enables the user to handle, process and manipulate fluids of very small volumes. This technology permits the integration of multiple laboratory applications into one single microfabricated chip, requires minimal manual user intervention and sample consumption, and allows enhanced data analysis speed and precision. Due to these numerous advantages, the potential for this technology to be applied in fundamental biop...

  7. Review and application of group theory to molecular systems biology

    Directory of Open Access Journals (Sweden)

    Rietman Edward A

    2011-06-01

    Full Text Available Abstract In this paper we provide a review of selected mathematical ideas that can help us better understand the boundary between living and non-living systems. We focus on group theory and abstract algebra applied to molecular systems biology. Throughout this paper we briefly describe possible open problems. In connection with the genetic code we propose that it may be possible to use perturbation theory to explore the adjacent possibilities in the 64-dimensional space-time manifold of the evolving genome. With regards to algebraic graph theory, there are several minor open problems we discuss. In relation to network dynamics and groupoid formalism we suggest that the network graph might not be the main focus for understanding the phenotype but rather the phase space of the network dynamics. We show a simple case of a C6 network and its phase space network. We envision that the molecular network of a cell is actually a complex network of hypercycles and feedback circuits that could be better represented in a higher-dimensional space. We conjecture that targeting nodes in the molecular network that have key roles in the phase space, as revealed by analysis of the automorphism decomposition, might be a better way to drug discovery and treatment of cancer.

  8. Cystitis: From Urothelial Cell Biology to Clinical Applications

    Directory of Open Access Journals (Sweden)

    Gilho Lee

    2014-01-01

    Full Text Available Cystitis is a urinary bladder disease with many causes and symptoms. The severity of cystitis ranges from mild lower abdominal discomfort to life-threatening haemorrhagic cystitis. The course of disease is often chronic or recurrent. Although cystitis represents huge economical and medical burden throughout the world and in many cases treatments are ineffective, the mechanisms of its origin and development as well as measures for effective treatment are still poorly understood. However, many studies have demonstrated that urothelial dysfunction plays a crucial role. In the present review we first discuss fundamental issues of urothelial cell biology, which is the core for comprehension of cystitis. Then we focus on many forms of cystitis, its current treatments, and advances in its research. Additionally we review haemorrhagic cystitis with one of the leading causative agents being chemotherapeutic drug cyclophosphamide and summarise its management strategies. At the end we describe an excellent and widely used animal model of cyclophosphamide induced cystitis, which gives researches the opportunity to get a better insight into the mechanisms involved and possibility to develop new therapy approaches.

  9. Computing Technologies for Oriented Education: Applications in Biological Sciences

    Directory of Open Access Journals (Sweden)

    Santiago Jaime Reyes

    2005-12-01

    Full Text Available The experience developing modern digital programs with highly qualified profesoors with several years of teaching postgraduate biological sciences matters is described. A small group of selected professors with a minimum knowledged or basic domain in computer software were invited to develop digital programs in the items of their interest,the purpose is to establish the bases for construction of an available digital library. The products to develop are a series of CD-ROM with program source in HTML format. The didactic strategy responds to a personal tutorship, step by step workshop, to build its own project (without programming languages. The workshop begins generating trust in very simple activities. It is designed to learn building and to advance evaluating the progress. It is fulfilled the necessity to put up-to-date the available material that regularly uses to impart the classes (video, slides, pictures, articles, examples etc. The information and computing technologies ICT are a indispensable tool to diffuse the knowledge to a coarser and more diverse public in the topics of their speciality. The obtained products are 8 CD ROM with didactic programs designed with scientific and technological bases.

  10. Application of the Raven UAV for chemical and biological detection

    Science.gov (United States)

    Altenbaugh, Ryan; Barton, Jeff; Chiu, Christopher; Fidler, Ken; Hiatt, Dan; Hawthorne, Chad; Marshall, Steven; Mohos, Joe; McHugh, Vince; Nicoloff, Bill

    2010-04-01

    This paper presents the plume tracking algorithms developed for a series of outdoor chemical-stimulant testing conducted at Dugway Proving Ground in 2008 and 2009 employing a Raven UAV equipped with a real-time chemical sensor. The flights were conducted as part of the a program under the sponsorship of the Army JPM NBC Contamination Avoidance and in conjunction with the Army PM-Unmanned Aircraft Systems, the Defense Threat Reduction Agency, and Edgewood Chemical Biological Center. This test demonstrated the Raven's ability to autonomously detect and track a chemical plume during a variety of atmospheric conditions. During the testing, the Raven conducted over a dozen flights, tracking outdoor releases of simulated chemical weapons over significant distances. The Raven was cued to the releases with standoff detection systems through Cursor on Target messages. Upon reaching the plume, the Raven used on-board sensors and on-board meteorological data to track the plume autonomously and determine the extent of the plume. Results were provided in real-time to the UAV operator.

  11. Molecular biology of breast cancer stem cells: potential clinical applications.

    Science.gov (United States)

    Nguyen, Nam P; Almeida, Fabio S; Chi, Alex; Nguyen, Ly M; Cohen, Deirdre; Karlsson, Ulf; Vinh-Hung, Vincent

    2010-10-01

    Breast cancer stem cells (CSC) have been postulated recently as responsible for failure of breast cancer treatment. The purpose of this study is to review breast CSCs molecular biology with respect to their mechanism of resistance to conventional therapy, and to develop treatment strategies that may improve survival of breast cancer patients. A literature search has identified in vitro and in vivo studies of breast CSCs. Breast CSCs overexpress breast cancer resistance protein (BCRP) which allows cancer cells to transport actively chemotherapy agents out of the cells. Radioresistance is modulated through activation of Wnt signaling pathway and overexpression of genes coding for glutathione. Lapatinib can selectively target HER-2 positive breast CSCs and improves disease-free survival in these patients. Metformin may target basal type breast CSCs. Parthenolide and oncolytic viruses are promising targeting agents for breast CSCs. Future clinical trials for breast cancer should include anti-cancer stem cells targeting agents in addition to conventional chemotherapy. Hypofractionation radiotherapy may be indicated for residual disease post chemotherapy.

  12. Compact THz FELs and Their Potential in Biological Applications

    CERN Document Server

    Gallerano, G P; Giovenale, E; Messina, G; Spassovsky, I P

    2005-01-01

    Two THz FEL sources are available at ENEA-Frascati covering the spectral range from 90 GHz to 0.7 THz. The first source, the ENEA Compact FEL, is based on a 5 MeV Microtron providing 4 A of peak current in 13 ps bunches. Peak power in excess of 3kW is obtained at 130 GHz. When the beam is focused, a peak E-field greater than 2 kV/cm can be obtained in the micropulse. The second source, FEL-CATS, is based on a 2.5 MeV RF Linac. After the Linac electrons enter a RF device that correlates their distribution in energy and phase. As a result a strong coherent spontaneous emission occurs in the undulator. Power up to several kW has been measured in the macropulse. The absence of a resonator results in a broad band emission from 0.4 to 0.7 THz. The peculiar temporal structure of the emitted radiation allows the investigation of the effects of high peak power, while maintaining a low average power incident on the sample. A variety of biological systems have been studied with the ENEA Compact FEL in the frame of the E...

  13. 6th International Conference on Practical Applications of Computational Biology & Bioinformatics

    CERN Document Server

    Luscombe, Nicholas; Fdez-Riverola, Florentino; Rodríguez, Juan; Practical Applications of Computational Biology & Bioinformatics

    2012-01-01

    The growth in the Bioinformatics and Computational Biology fields over the last few years has been remarkable.. The analysis of the datasets of Next Generation Sequencing needs new algorithms and approaches from fields such as Databases, Statistics, Data Mining, Machine Learning, Optimization, Computer Science and Artificial Intelligence. Also Systems Biology has also been emerging as an alternative to the reductionist view that dominated biological research in the last decades. This book presents the results of the  6th International Conference on Practical Applications of Computational Biology & Bioinformatics held at University of Salamanca, Spain, 28-30th March, 2012 which brought together interdisciplinary scientists that have a strong background in the biological and computational sciences.

  14. Multimodal Molecular Mass Spectrometry Imaging : Development and Applications in Plant Biology and Forensic Toxicology

    OpenAIRE

    Porta, Tiffany

    2013-01-01

    This thesis focuses on the development of new analytical platforms for molecular mass spectrometry imaging and their applications in plant biology and forensic toxicology. So far, in drug metabolism or forensic toxicology, liquid chromatography with mass spectrometric detection is the technique of choice for analyzing drugs and metabolites in complex biological samples. LC-MS remains however challenging, because the development of appropriate sample preparation requires complex and time-consu...

  15. Patents and licensing in pharmaceutical industry

    Directory of Open Access Journals (Sweden)

    Katerina Ancevska Netkovska

    2005-05-01

    Full Text Available Intellectual property rights (IPR have been defined as ideas, inventions, and creative expressions based on which there is a public willingness to bestow the status of property. IPR provide certain exclusive rights to the inventors or creators of that property, in order to enable them to reap commercial benefits from their creative efforts or reputation. There are several types of intellectual property protection like patent, copyright, trademark, etc. Patent is recognition for an invention, which satisfies the criteria of global novelty, non-obviousness, and industrial application. IPR is prerequisite for better identification, planning, commercialization, rendering, and thereby protection of invention or creativity. Each industry should evolve its own IPR policies, management style, strategies, and so on depending on its area of specialty. Pharmaceutical industry currently has an evolving IPR strategy requiring a better focus and approach in the coming era. The protection of inventions with patents in the pharmaceutical industry have a specific role in the development of society and represent one of the drivers of economic development. The license agreements are considered as one of the most common types of transfer of industrial property rights. The right holders often transfer their rights to patents by concluding licensing agreement. While the patent license may give the license a right to use the technology many license agreements have provisions for the transfer of know-how in addition to the patent.

  16. Human mesenchymal stem cells: from basic biology to clinical applications

    DEFF Research Database (Denmark)

    Abdallah, B M; Kassem, M

    2008-01-01

    Mesenchymal stem cells (MSC) are a group of clonogenic cells present among the bone marrow stroma and capable of multilineage differentiation into mesoderm-type cells such as osteoblasts, adipocytes and chondrocytes. Due to their ease of isolation and their differentiation potential, MSC are being...... introduced into clinical medicine in variety of applications and through different ways of administration. Here, we discuss approaches for isolation, characterization and directing differentiation of human mesenchymal stem cells (hMSC). An update of the current clinical use of the cells is also provided....

  17. Applications of PIXE analysis to studies in cell biology

    Energy Technology Data Exchange (ETDEWEB)

    Matsumoto, Shinji; Furuse, Masako; Kitao, Kensuke [National Inst. of Radiological Sciences, Chiba (Japan); Ishihara, Toyoyuki; Shima, Kunihiro; Chiba, Ren

    1992-12-31

    PIXE application to the measurement of cellular elements is outlined on characteristic variations of the contents of yeast, CHO, V79 and MM46 cultured mammalian cells according to environmental changes. Cellular elements from P to Br were successfully analyzed at these cells after cautious preparation of samples in filtration steps with as possible as less deterioration. We confirmed linear relationships between the X-ray yields and these element contents. The experiment was extended to an analysis of cellular substances at molecular level by scanning of specimens. Preliminary results were included. (author).

  18. Biological Immune System Applications on Mobile Robot for Disabled People

    Directory of Open Access Journals (Sweden)

    Songmin Jia

    2014-01-01

    Full Text Available To improve the service quality of service robots for the disabled, immune system is applied on robot for its advantages such as diversity, dynamic, parallel management, self-organization, and self-adaptation. According to the immune system theory, local environment condition sensed by robot is considered an antigen while robot is regarded as B-cell and possible node as antibody, respectively. Antibody-antigen affinity is employed to choose the optimal possible node to ensure the service robot can pass through the optimal path. The paper details the immune system applications on service robot and gives experimental results.

  19. Characteristics of silver nanoparticles in vehicles for biological applications.

    Science.gov (United States)

    Kejlová, Kristina; Kašpárková, Věra; Krsek, Daniel; Jírová, Dagmar; Kolářová, Hana; Dvořáková, Markéta; Tománková, Kateřina; Mikulcová, Veronika

    2015-12-30

    Silver nanoparticles (AgNPs) have been used for decades as anti-bacterial agents in various industrial fields such as cosmetics, health industry, food storage, textile coatings and environmental applications, although their toxicity is not fully recognized yet. Antimicrobial and catalytic activity of AgNPs depends on their size as well as structure, shape, size distribution, and physico-chemical environment. The unique properties of AgNPs require novel or modified toxicological methods for evaluation of their toxic potential combined with robust analytical methods for characterization of nanoparticles applied in relevant vehicles, e.g., culture medium with/without serum and phosphate buffered saline.

  20. 47 CFR 13.17 - Replacement license.

    Science.gov (United States)

    2010-10-01

    ... Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL COMMERCIAL RADIO OPERATORS General § 13.17 Replacement... Operator License, Marine Radio Operator Permit, First Class Radiotelegraph Operator's Certificate, Second... Radio Operator's License, Restricted GMDSS Radio Operator License, GMDSS Radio Maintainer's License,...