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Sample records for biochemistry section laboratory

  1. Biochemistry: from supermarket to laboratory

    OpenAIRE

    F. R. Freitas-Rego; Pereira, M. G.; S. O. Loureiro; M. T. de Santana; R. G. Garrido; F. de S.R.G Garrido

    2007-01-01

    After new campi as Instituto Multidisciplinar em Saúde (IMS/UFBA) startedworking, it was necessary to develop practical classes using domestic reagents atBiochemistry to Pharmacy (IMS078). Firstly, students visited a supermarket to readnutritional information at label and select possible products to be used in class. Moreover,chemical processes and fermentation were discussed as different foods and drinks wereanalysed. Some food were token to laboratories so that biomole cules qualitative ana...

  2. Biochemistry: from supermarket to laboratory

    Directory of Open Access Journals (Sweden)

    F. R. Freitas-Rego

    2007-05-01

    Full Text Available After new campi as Instituto Multidisciplinar em Saúde (IMS/UFBA startedworking, it was necessary to develop practical classes using domestic reagents atBiochemistry to Pharmacy (IMS078. Firstly, students visited a supermarket to readnutritional information at label and select possible products to be used in class. Moreover,chemical processes and fermentation were discussed as different foods and drinks wereanalysed. Some food were token to laboratories so that biomole cules qualitative analysiswere carried on. Domestic use reagents as pharmaceutical iodine solutions, commercialNaOH and vegetable pigments were used. The substances identified were reductant glycid,starch, fatty acid, triacylglycerol and protein. Reactions allowed to identify fungi andvegetable tissues. Moreover, invertase and alfa-amilase activities were determined. Afterdiscussions in class, students could improve biochemical knowledge as well as distinguishbetween milk or lactic drink, animal fat or vegetable hydrogenated fat, honey or glucose.After that, students produced kits and wrote laboratorial notes for use in classes with therest of the group.

  3. Immobilized Lactase in the Biochemistry Laboratory

    Science.gov (United States)

    Allison, Matthew J.; Bering, C. Larry

    1998-10-01

    Immobilized enzymes have many practical applications. They may be used in clinical, industrial, and biotechnological laboratories and in many clinical diagnostic kits. For educational purposes, use of immobilized enzymes can easily be taught at the undergraduate or even secondary level. We have developed an immobilized enzyme experiment that combines many practical techniques used in the biochemistry laboratory and fits within a three-hour time frame. In this experiment, lactase from over-the-counter tablets for patients with lactose intolerance is immobilized in polyacrylamide, which is then milled into small beads and placed into a chromatography column. A lactose solution is added to the column and the eluant is assayed using the glucose oxidase assay, available as a kit. We have determined the optimal conditions to give the greatest turnover of lactose while allowing the immobilized enzymes to be active for long periods at room temperature.

  4. An Integrated Biochemistry Laboratory, Including Molecular Modeling

    Science.gov (United States)

    Hall, Adele J. Wolfson Mona L.; Branham, Thomas R.

    1996-11-01

    The dilemma of designing an advanced undergraduate laboratory lies in the desire to teach and reinforce basic principles and techniques while at the same time exposing students to the excitement of research. We report here on a one-semester, project-based biochemistry laboratory that combines the best features of a cookbook approach (high success rate, achievement of defined goals) with those of an investigative, discovery-based approach (student involvement in the experimental design, excitement of real research). Individual modules may be selected and combined to meet the needs of different courses and different institutions. The central theme of this lab is protein purification and design. This laboratory accompanies the first semester of biochemistry (Structure and Function of Macromolecules, a course taken mainly by junior and senior chemistry and biological chemistry majors). The protein chosen as the object of study is the enzyme lysozyme, which is utilized in all projects. It is suitable for a student lab because it is easily and inexpensively obtained from egg white and is extremely stable, and its high isoelectric point (pI = 11) allows for efficient separation from other proteins by ion-exchange chromatography. Furthermore, a literature search conducted by the resourceful student reveals a wealth of information, since lysozyme has been the subject of numerous studies. It was the first enzyme whose structure was determined by crystallography (1). Hendrickson et al. (2) have previously described an intensive one-month laboratory course centered around lysozyme, although their emphasis is on protein stability rather than purification and engineering. Lysozyme continues to be the focus of much exciting new work on protein folding and dynamics, structure and activity (3 - 5). This lab course includes the following features: (i) reinforcement of basic techniques, such as preparation of buffers, simple enzyme kinetics, and absorption spectroscopy; (ii

  5. An "in Silico" DNA Cloning Experiment for the Biochemistry Laboratory

    Science.gov (United States)

    Elkins, Kelly M.

    2011-01-01

    This laboratory exercise introduces students to concepts in recombinant DNA technology while accommodating a major semester project in protein purification, structure, and function in a biochemistry laboratory for junior- and senior-level undergraduate students. It is also suitable for forensic science courses focused in DNA biology and advanced…

  6. Re-Attitude of Biochemistry Laboratory Course Contents in Medical, Dentistry and Para-Medical Faculties

    Directory of Open Access Journals (Sweden)

    Durdi Qujeq

    2014-04-01

    Full Text Available Regarding to development of basic science and new methods in biochemistry in recent years, practical biochemistry contents should be optimized (1. Therefore, re-attitude biochemistry course contents in medical schools has paralleled worldwide trends a moved from current status is needed (2, 3. As reported by investi-gators many medical schools around the world have reformed their medical curriculum in recent years (2. Many authors are convinced that students learn more effectively if the knowledge and skills they acquire are inserted and contextualized in relevant real-life, problem based situations (3. Previous studies demonstrated that biochemistry course content is now incorporated into the clinical beneficial, therefore the evolution of students in many countries has occurred (4. In this respect, the purpose of the current study was to evaluate biochemistry laboratory course contents in medical, dentistry and Para-medicine faculties from view points of the students. This descriptive study was per-formed in years 2010-2013. Questionnaire contained items about appropriateness of biochemistry laboratory contents for students. The first section of questionnaire determines the effectiveness of bioche¬mistry laboratory contents and the factors influence on it. The second section indicates the application of biochemistry laboratory contents, and the third parts of questi¬onnaire demonstrate the laboratory time spent of biochemistry laboratory course contents. For this purpose three faculties were selected. First, Faculty of Medicine (50 medical students, second Faculty of Dentistry (50 dentistry students and third Faculty of Para-medicine (50 laboratory science students were selected. At least 50 students were selected from each faculty and they received a questionnaire. All students were randomly selected to receive a standard questionnaire designed to evaluate their opinions about biochemistry laboratory course contents. The students were asked

  7. Raising environmental awareness through applied biochemistry laboratory experiments.

    Science.gov (United States)

    Salman Ashraf, S

    2013-01-01

    Our environment is under constant pressure and threat from various sources of pollution. Science students, in particular chemistry students, must not only be made aware of these issues, but also be taught that chemistry (and science) can provide solutions to such real-life issues. To this end, a newly developed biochemistry laboratory experiment is described that guides students to learn about the applicability of peroxidase enzymes to degrade organic dyes (as model pollutants) in simulated waste water. In addition to showing how enzymes can potentially be used for waste water remediation, various factors than can affect enzyme-based reactions such as pH, temperature, concentration of substrates/enzymes, and denaturants can also be tested. This "applied biotechnology" experiment was successfully implemented in an undergraduate biochemistry laboratory course to enhance students' learning of environmental issues as well important biochemistry concepts. Student survey confirmed that this laboratory experiment was successful in achieving the objectives of raising environmental awareness in students and illustrating the usefulness of chemistry in solving real-life problems. This experiment can be easily adopted in an introductory biochemistry laboratory course and taught as an inquiry-guided exercise.

  8. Ribose 5-Phosphate Isomerase Investigations for the Undergraduate Biochemistry Laboratory

    Science.gov (United States)

    Jewett, Kathy; Sandwick, Roger K.

    2011-01-01

    The enzyme ribose 5-phosphate isomerase (RpiA) has many features that make it attractive as a focal point of a semester-long, advanced biochemistry laboratory for undergraduate students. The protein can easily and inexpensively be isolated from spinach using traditional purification techniques. Characterization of RpiA enzyme activity can be…

  9. Differentiating Biochemistry Course Laboratories Based on Student Experience

    Science.gov (United States)

    Jakubowski, Henry V.

    2011-01-01

    Content and emphases in undergraduate biochemistry courses can be readily tailored to accommodate the standards of the department in which they are housed, as well as the backgrounds of the students in the courses. A more challenging issue is how to construct laboratory experiences for a class with both chemistry majors, who usually have little or…

  10. Raising Environmental Awareness through Applied Biochemistry Laboratory Experiments

    Science.gov (United States)

    Salman Ashraf, S.

    2013-01-01

    Our environment is under constant pressure and threat from various sources of pollution. Science students, in particular chemistry students, must not only be made aware of these issues, but also be taught that chemistry (and science) can provide solutions to such real-life issues. To this end, a newly developed biochemistry laboratory experiment…

  11. Error tracking in a clinical biochemistry laboratory

    DEFF Research Database (Denmark)

    Szecsi, Pal Bela; Ødum, Lars

    2009-01-01

    BACKGROUND: We report our results for the systematic recording of all errors in a standard clinical laboratory over a 1-year period. METHODS: Recording was performed using a commercial database program. All individuals in the laboratory were allowed to report errors. The testing processes were cl...

  12. Virtual Laboratories in Chemistry, Biochemistry, & Molecular Biology

    DEFF Research Database (Denmark)

    May, Michael; Achiam, Marianne

    2013-01-01

    Report (state-of-the-art review) from a research and development project on virtual laboratories supported by Markedmodningsfonden (tidl. "Fornyelsesfonden")(2012-2014). http://markedsmodningsfonden.dk/projekt/0/34/495....

  13. A biochemistry laboratory course designed to enhance students autonomy

    Directory of Open Access Journals (Sweden)

    T. Silva

    2015-08-01

    Full Text Available INTRODUCTION: Laboratory sessions are responsible for promoting instrumentation skills desirable in biochemistry and biochemistry related careers. They are traditionally based on experimental protocols that lead to the expected results, and students usually have not autonomy to plan and execute their experiments. GOALS: This work aimed to enhance a traditional biochemistry lab course, applying pre-lab quizzes on protein biochemistry and lab techniques in order to have students better prepared to plan, execute and interpret experiments. This approach also aims to bring the laboratory sessions into an inquiry-based environment capable to improve students’ independent capabilities in 2 autonomy domains: learning and communication. MATERIAL AND METHODS: Online quizzes are delivered one week before each laboratory session, containing questions regarding the experimental techniques and theoretical basis related to them. Laboratory activities are presented in an inquiry-based approach where the first class of each activity is dedicated to plan experiments in order to answer the research questions presented by instructors. Activities are also organized in order to enhance students’ autonomy. The first activity is the simplest and more instructor-controlled and the last one is the most complex and less driven, transferring gradually to students the responsibility for their decisions in laboratory, supporting students’ autonomy. RESULTS: Online quizzes allowed instructors to identify students’ difficulties and to timely intervene. Scientific reports presented by students at the end of each activity showed that they performed better on less driven activities in which autonomy support were more complex than in the instructor controlled activities. CONCLUSIONS: Scientific reports analysis reveals students capabilities related to different scopes of autonomy, such as: discuss different strategies; find multiple solutions to solve problems; make their

  14. 78 FR 4170 - License Amendment Request for Analytical Bio-Chemistry Laboratories, Inc., Columbia, MO

    Science.gov (United States)

    2013-01-18

    ... COMMISSION License Amendment Request for Analytical Bio-Chemistry Laboratories, Inc., Columbia, MO AGENCY... issuance of a license amendment to Materials License No. 24-13365-01 issued to Analytical Bio-Chemistry... accession numbers are: 1. Analytical Bio-Chemistry Laboratories, Inc., Licensee amendment request...

  15. Myoglobin structure and function: A multiweek biochemistry laboratory project.

    Science.gov (United States)

    Silverstein, Todd P; Kirk, Sarah R; Meyer, Scott C; Holman, Karen L McFarlane

    2015-01-01

    We have developed a multiweek laboratory project in which students isolate myoglobin and characterize its structure, function, and redox state. The important laboratory techniques covered in this project include size-exclusion chromatography, electrophoresis, spectrophotometric titration, and FTIR spectroscopy. Regarding protein structure, students work with computer modeling and visualization of myoglobin and its homologues, after which they spectroscopically characterize its thermal denaturation. Students also study protein function (ligand binding equilibrium) and are instructed on topics in data analysis (calibration curves, nonlinear vs. linear regression). This upper division biochemistry laboratory project is a challenging and rewarding one that not only exposes students to a wide variety of important biochemical laboratory techniques but also ties those techniques together to work with a single readily available and easily characterized protein, myoglobin.

  16. An in silico DNA cloning experiment for the biochemistry laboratory.

    Science.gov (United States)

    Elkins, Kelly M

    2011-01-01

    This laboratory exercise introduces students to concepts in recombinant DNA technology while accommodating a major semester project in protein purification, structure, and function in a biochemistry laboratory for junior- and senior-level undergraduate students. It is also suitable for forensic science courses focused in DNA biology and advanced high school biology classes. Students begin by examining a plasmid map with the goal of identifying which restriction enzymes may be used to clone a piece of foreign DNA containing a gene of interest into the vector. From the National Center for Biotechnology Initiative website, students are instructed to retrieve a protein sequence and use Expasy's Reverse Translate program to reverse translate the protein to cDNA. Students then use Integrated DNA Technologies' OligoAnalyzer to predict the complementary DNA strand and obtain DNA recognition sequences for the desired restriction enzymes from New England Biolabs' website. Students add the appropriate DNA restriction sequences to the double-stranded foreign DNA for cloning into the plasmid and infecting Escherichia coli cells. Students are introduced to computational biology tools, molecular biology terminology and the process of DNA cloning in this valuable single session, in silico experiment. This project develops students' understanding of the cloning process as a whole and contrasts with other laboratory and internship experiences in which the students may be involved in only a piece of the cloning process/techniques. Students interested in pursuing postgraduate study and research or employment in an academic biochemistry or molecular biology laboratory or industry will benefit most from this experience.

  17. [External quality assessment in clinical biochemistry laboratories: pilot study in 11 laboratories of Lomé (Togo)].

    Science.gov (United States)

    Kouassi, Kafui; Fétéké, Lochina; Assignon, Selom; Dorkenoo, Ameyo; Napo-Koura, Gado

    2015-01-01

    This study aims to evaluate the performance of a few biochemistry analysis and make recommendations to the place of the stakeholders. It is a cross-sectional study conducted between the October 1(st), 2012 and the July 31, 2013 bearing on the results of 5 common examinations of clinical biochemistry, provided by 11 laboratories volunteers opening in the public and private sectors. These laboratories have analysed during the 3 cycles, 2 levels (medium and high) of serum concentration of urea, glucose, creatinine and serum aminotransferases. The performance of laboratories have been determined from the acceptable limits corresponding to the limits of total errors, defined by the French Society of Clinical Biology (SFBC). A system of internal quality control is implemented by all laboratories and 45% of them participated in international programs of external quality assessment (EQA). The rate of acceptable results for the entire study was of 69%. There was a significant difference (p<0.002) between the performance of the group of laboratories engaged in a quality approach and the group with default implementation of the quality approach. Also a significant difference was observed between the laboratories of the central level and those of the peripheral level of our health system (p<0.047). The performance of the results provided by the laboratories remains relatively unsatisfactory. It is important that the Ministry of Health put in place a national program of EQA with mandatory participation.

  18. Known Structure, Unknown Function: An Inquiry-Based Undergraduate Biochemistry Laboratory Course

    Science.gov (United States)

    Gray, Cynthia; Price, Carol W.; Lee, Christopher T.; Dewald, Alison H.; Cline, Matthew A.; McAnany, Charles E.; Columbus, Linda; Mura, Cameron

    2015-01-01

    Undergraduate biochemistry laboratory courses often do not provide students with an authentic research experience, particularly when the express purpose of the laboratory is purely instructional. However, an instructional laboratory course that is inquiry- and research-based could simultaneously impart scientific knowledge and foster a student's…

  19. Guided Inquiry in a Biochemistry Laboratory Course Improves Experimental Design Ability

    Science.gov (United States)

    Goodey, Nina M.; Talgar, Cigdem P.

    2016-01-01

    Many biochemistry laboratory courses expose students to laboratory techniques through pre-determined experiments in which students follow stepwise protocols provided by the instructor. This approach fails to provide students with sufficient opportunities to practice experimental design and critical thinking. Ten inquiry modules were created for a…

  20. A Semester-Long Project-Oriented Biochemistry Laboratory Based on "Helicobacter pylori" Urease

    Science.gov (United States)

    Farnham, Kate R.; Dube, Danielle H.

    2015-01-01

    Here we present the development of a 13 week project-oriented biochemistry laboratory designed to introduce students to foundational biochemical techniques and then enable students to perform original research projects once they have mastered these techniques. In particular, we describe a semester-long laboratory that focuses on a biomedically…

  1. Thermodynamic Exploration of Eosin-Lysozyme Binding: A Physical Chemistry and Biochemistry Laboratory Experiment

    Science.gov (United States)

    Huisman, Andrew J.; Hartsell, Lydia R.; Krueger, Brent P.; Pikaart, Michael J.

    2010-01-01

    We developed a modular pair of experiments for use in the undergraduate physical chemistry and biochemistry laboratories. Both experiments examine the thermodynamics of the binding of a small molecule, eosin Y, to the protein lysozyme. The assay for binding is the quenching of lysozyme fluorescence by eosin through resonant energy transfer. In…

  2. Forensic Analysis of Canine DNA Samples in the Undergraduate Biochemistry Laboratory

    Science.gov (United States)

    Carson, Tobin M.; Bradley, Sharonda Q.; Fekete, Brenda L.; Millard, Julie T.; LaRiviere, Frederick J.

    2009-01-01

    Recent advances in canine genomics have allowed the development of highly distinguishing methods of analysis for both nuclear and mitochondrial DNA. We describe a laboratory exercise suitable for an undergraduate biochemistry course in which the polymerase chain reaction is used to amplify hypervariable regions of DNA from dog hair and saliva…

  3. Evaluation of a Modified Keller Method in a Biochemistry Laboratory Course.

    Science.gov (United States)

    Jackman, Lance E.

    1982-01-01

    Describes a modified Keller plan for teaching a biochemistry laboratory course and evaluates the method's effectiveness by comparing results of students using Keller plan (N=17) to students receiving traditional instruction (N=16). Results suggest the modified Keller plan is a successful instructional method for the course. (Author/SK)

  4. Teaching Protein Purification and Characterization Techniques: A Student-Initiated, Project-Oriented Biochemistry Laboratory Course

    Science.gov (United States)

    MacDonald, Gina

    2008-01-01

    This report describes a biochemistry laboratory that is completely project-oriented. Upper-level biology and chemistry majors work in teams to purify a protein of their choice. After the student groups have completed literature searches, ordered reagents, and made buffers they continue to learn basic protein purification and biochemical techniques…

  5. An SDS-PAGE Examination of Protein Quaternary Structure and Disulfide Bonding for a Biochemistry Laboratory

    Science.gov (United States)

    Powers, Jennifer L.; Andrews, Carla S.; St. Antoine, Caroline C.; Jain, Swapan S.; Bevilacqua, Vicky L. H.

    2005-01-01

    Electrophoresis is a valuable tool for biochemists, yet this technique is often not included in biochemistry laboratory curricula owing to time constraints or lack of equipment. Protein structure is also a topic of interest in many disciplines, yet most undergraduate lab experiments focus only on primary structure. In this experiment, students use…

  6. Integrating Bio-Inorganic and Analytical Chemistry into an Undergraduate Biochemistry Laboratory

    Science.gov (United States)

    Erasmus, Daniel J.; Brewer, Sharon E.; Cinel, Bruno

    2015-01-01

    Undergraduate laboratories expose students to a wide variety of topics and techniques in a limited amount of time. This can be a challenge and lead to less exposure to concepts and activities in bio-inorganic chemistry and analytical chemistry that are closely-related to biochemistry. To address this, we incorporated a new iron determination by…

  7. Biochemistry Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Biochemistry Facility provides expert services and consultation in biochemical enzyme assays and protein purification. The facility currently features 1) Liquid...

  8. Known Structure, Unknown Function: An Inquiry-based Undergraduate Biochemistry Laboratory Course

    CERN Document Server

    Gray, Cynthia; Lee, Christopher T; Dewald, Alison H; Cline, Matthew A; McAnany, Charles E; Columbus, Linda; Mura, Cameron

    2015-01-01

    Undergraduate biochemistry laboratory courses often do not provide students with an authentic research experience, particularly when the express purpose of the laboratory is purely instructional. However, an instructional laboratory course that is inquiry- and research-based could simultaneously impart scientific knowledge and foster a student's research expertise and confidence. We have developed a year-long undergraduate biochemistry laboratory curriculum wherein students determine, via experiment and computation, the function of a protein of known three-dimensional structure. The first half of the course is inquiry-based and modular in design; students learn general biochemical techniques while gaining preparation for research experiments in the second semester. Having learned standard biochemical methods in the first semester, students independently pursue their own (original) research projects in the second semester. This new curriculum has yielded an improvement in student performance and confidence as ...

  9. Director, Laboratory Animal Care and Use Section

    Data.gov (United States)

    Federal Laboratory Consortium — The NIAMS Laboratory Animal Care and Use Section (LACU) provides support to all NIAMS Intramural Research Program (IRP) Branches and Laboratories using animals. The...

  10. A semester-long project-oriented biochemistry laboratory based on Helicobacter pylori urease.

    Science.gov (United States)

    Farnham, Kate R; Dube, Danielle H

    2015-01-01

    Here we present the development of a 13 week project-oriented biochemistry laboratory designed to introduce students to foundational biochemical techniques and then enable students to perform original research projects once they have mastered these techniques. In particular, we describe a semester-long laboratory that focuses on a biomedically relevant enzyme--Helicobacter pylori (Hp) urease--the activity of which is absolutely required for the gastric pathogen Hp to colonize the human stomach. Over the course of the semester, students undertake a biochemical purification of Hp urease, assess the success of their purification, and investigate the activity of their purified enzyme. In the final weeks of the semester, students design and implement their own experiments to study Hp urease. This laboratory provides students with an understanding of the importance of biochemistry in human health while empowering them to engage in an active area of research.

  11. Known structure, unknown function: An inquiry-based undergraduate biochemistry laboratory course.

    Science.gov (United States)

    Gray, Cynthia; Price, Carol W; Lee, Christopher T; Dewald, Alison H; Cline, Matthew A; McAnany, Charles E; Columbus, Linda; Mura, Cameron

    2015-01-01

    Undergraduate biochemistry laboratory courses often do not provide students with an authentic research experience, particularly when the express purpose of the laboratory is purely instructional. However, an instructional laboratory course that is inquiry- and research-based could simultaneously impart scientific knowledge and foster a student's research expertise and confidence. We have developed a year-long undergraduate biochemistry laboratory curriculum wherein students determine, via experiment and computation, the function of a protein of known three-dimensional structure. The first half of the course is inquiry-based and modular in design; students learn general biochemical techniques while gaining preparation for research experiments in the second semester. Having learned standard biochemical methods in the first semester, students independently pursue their own (original) research projects in the second semester. This new curriculum has yielded an improvement in student performance and confidence as assessed by various metrics. To disseminate teaching resources to students and instructors alike, a freely accessible Biochemistry Laboratory Education resource is available at http://biochemlab.org.

  12. Drug Synthesis and Analysis on a Dime: A Capstone Medicinal Chemistry Experience for the Undergraduate Biochemistry Laboratory

    Science.gov (United States)

    Streu, Craig N.; Reif, Randall D.; Neiles, Kelly Y.; Schech, Amanda J.; Mertz, Pamela S.

    2016-01-01

    Integrative, research-based experiences have shown tremendous potential as effective pedagogical approaches. Pharmaceutical development is an exciting field that draws heavily on organic chemistry and biochemistry techniques. A capstone drug synthesis/analysis laboratory is described where biochemistry students synthesize azo-stilbenoid compounds…

  13. Kinetics of Papain: An Introductory Biochemistry Laboratory Experiment

    Science.gov (United States)

    Cornely, Kathleen; Crespo, Eric; Earley, Michael; Kloter, Rachel; Levesque, Aime; Pickering, Mary

    1999-05-01

    Enzyme kinetics experiments are popular in the undergraduate laboratory. These experiments have pedagogic value because they reinforce the concepts of Michaelis-Menten kinetics covered in the lecture portion of the course and give students the experience of calculating kinetic constants from data they themselves have generated. In this experiment, we investigate the kinetics of the thiol protease papain. The source of the papain is commercially available papaya latex. A specific substrate, Na-benzoyl-arginine-p-nitroanilide (BAPNA), is used, which takes advantage of the fact that papain interacts with a phenylalanine residue two amino acids away from the peptide bond cleaved. Upon hydrolysis by papain, a bright yellow product is released, p-nitroaniline. This allows the reaction to be monitored spectrophotometrically by measuring the rate of formation of the p-nitroaniline product as a function of the increase in absorbance of the solution at the lmax of p-nitroaniline (400 nm) over time at various substrate concentrations. These data are used to plot a Lineweaver-Burk plot from which the vmax and KM are obtained. If time permits, students carry out additional investigations in which e of p-nitroaniline is measured, the enzyme solution protein concentration is measured, the enzyme purity is evaluated by SDS-PAGE, and a pH-rate profile is constructed from experimental data.

  14. Application of indices Cp and Cpk to improve quality control capability in clinical biochemistry laboratories.

    Science.gov (United States)

    Chen, Ming-Shu; Wu, Ming-Hsun; Lin, Chih-Ming

    2014-04-30

    The traditional criteria for acceptability of analytic quality may not be objective in clinical laboratories. To establish quality control procedures intended to enhance Westgard multi-rules for improving the quality of clinical biochemistry tests, we applied the Cp and Cpk quality-control indices to monitor tolerance fitting and systematic variation of clinical biochemistry test results. Daily quality-control data of a large Taiwanese hospital in 2009 were analyzed. The test items were selected based on an Olympus biochemistry machine and included serum albumin, aspartate aminotransferase, cholesterol, glucose and potassium levels. Cp and Cpk values were calculated for normal and abnormal levels, respectively. The tolerance range was estimated with data from 50 laboratories using the same instruments and reagents. The results showed a monthly trend of variation for the five items under investigation. The index values of glucose were lower than those of the other items, and their values were usually <2. In contrast to the Cp value for cholesterol, Cpk of cholesterol was lower than 2, indicating a systematic error that should be further investigated. This finding suggests a degree of variation or failure to meet specifications that should be corrected. The study indicated that Cp and Cpk could be applied not only for monitoring variations in quality control, but also for revealing inter-laboratory qualitycontrol capability differences.

  15. National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines for use of tumor markers in clinical practice

    DEFF Research Database (Denmark)

    Sturgeon, Catharine M; Hoffman, Barry R; Chan, Daniel W

    2008-01-01

    BACKGROUND: This report presents updated National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines summarizing quality requirements for the use of tumor markers. METHODS: One subcommittee developed guidelines for analytical quality relevant to serum and tissue-based tumor...

  16. Integrating bio-inorganic and analytical chemistry into an undergraduate biochemistry laboratory.

    Science.gov (United States)

    Erasmus, Daniel J; Brewer, Sharon E; Cinel, Bruno

    2015-01-01

    Undergraduate laboratories expose students to a wide variety of topics and techniques in a limited amount of time. This can be a challenge and lead to less exposure to concepts and activities in bio-inorganic chemistry and analytical chemistry that are closely-related to biochemistry. To address this, we incorporated a new iron determination by atomic absorption spectroscopy exercise as part of a five-week long laboratory-based project on the purification of myoglobin from beef. Students were required to prepare samples for chemical analysis, operate an atomic absorption spectrophotometer, critically evaluate their iron data, and integrate these data into a study of myoglobin.

  17. Dishonesty in the biochemistry classroom laboratory: A synthesis of causes and prevention.

    Science.gov (United States)

    Del Carlo, Dawn; Bodner, George

    2006-09-01

    Although reports of academic cheating are abundant, there are relatively few papers in the literature that focus on cheating in the context of science courses and even fewer that address dishonest practices, such as "cooking" or fudging data, within the classroom laboratory. This paper briefly reviews the existing literature on academic dishonesty and explores two theories that can be used to explain why cheating occurs: (1) classroom goal structure and (2) attitudes of neutralization. We conclude with a discussion of the implications of these theories within the context of a biochemistry and molecular biology teaching laboratory.

  18. Fluorescence quantum yield measurements of fluorescent proteins: a laboratory experiment for a biochemistry or molecular biophysics laboratory course.

    Science.gov (United States)

    Wall, Kathryn P; Dillon, Rebecca; Knowles, Michelle K

    2015-01-01

    Fluorescent proteins are commonly used in cell biology to assess where proteins are within a cell as a function of time and provide insight into intracellular protein function. However, the usefulness of a fluorescent protein depends directly on the quantum yield. The quantum yield relates the efficiency at which a fluorescent molecule converts absorbed photons into emitted photons and it is necessary to know for assessing what fluorescent protein is the most appropriate for a particular application. In this work, we have designed an upper-level, biochemistry laboratory experiment where students measure the fluorescence quantum yields of fluorescent proteins relative to a standard organic dye. Four fluorescent protein variants, enhanced cyan fluorescent protein (ECFP), enhanced green fluorescent protein (EGFP), mCitrine, and mCherry, were used, however the methods described are useful for the characterization of any fluorescent protein or could be expanded to fluorescent quantum yield measurements of organic dye molecules. The laboratory is designed as a guided inquiry project and takes two, 4 hr laboratory periods. During the first day students design the experiment by selecting the excitation wavelength, choosing the standard, and determining the concentration needed for the quantum yield experiment that takes place in the second laboratory period. Overall, this laboratory provides students with a guided inquiry learning experience and introduces concepts of fluorescence biophysics into a biochemistry laboratory curriculum.

  19. Team-Based Learning, Faculty Research, and Grant Writing Bring Significant Learning Experiences to an Undergraduate Biochemistry Laboratory Course

    Science.gov (United States)

    Evans, Hedeel Guy; Heyl, Deborah L.; Liggit, Peggy

    2016-01-01

    This biochemistry laboratory course was designed to provide significant learning experiences to expose students to different ways of succeeding as scientists in academia and foster development and improvement of their potential and competency as the next generation of investigators. To meet these goals, the laboratory course employs three…

  20. Biochemistry Instrumentation Core Technology Center

    Data.gov (United States)

    Federal Laboratory Consortium — The UCLA-DOE Biochemistry Instrumentation Core Facility provides the UCLA biochemistry community with easy access to sophisticated instrumentation for a wide variety...

  1. Development of a Semester-Long, Inquiry-Based Laboratory Course in Upper-Level Biochemistry and Molecular Biology

    Science.gov (United States)

    Murthy, Pushpalatha P. N.; Thompson, Martin; Hungwe, Kedmon

    2014-01-01

    A semester-long laboratory course was designed and implemented to familiarize students with modern biochemistry and molecular biology techniques. The designed format involved active student participation, evaluation of data, and critical thinking, and guided students to become independent researchers. The first part of the course focused on…

  2. Equilibrium Gel Filtration Chromatography for the Measurement of Protein-Ligand Binding in the Undergraduate Biochemistry Laboratory

    Science.gov (United States)

    Craig, Douglas B.

    2005-01-01

    A laboratory exercise used in the senior biochemistry course at the University of Winnipeg for three years is discussed. It combines liquid chromatography and absorbance spectroscopy and also allows the students to produce a quantitative result within a single three-hour period.

  3. The Determination of Vitamin D-Dependent Calcium Binding Protein in Chick Intesting: An Undergraduate Biochemistry Laboratory Experiment.

    Science.gov (United States)

    Lessard, George M.

    1980-01-01

    Described is an experiment used in an undergraduate biochemistry laboratory involving inducing rickets in chicks and correlating the disease to a reduction in vitamin D-dependent calcium binding protein. Techniques involved are hormone induction, protein isolation, and radioisotope methodology. (Author/DS)

  4. HPLC of the Polypeptides in a Hydrolyzate of Egg-White Lysozyme. An Experiment for the Undergraduate Biochemistry Laboratory.

    Science.gov (United States)

    Richardson, W. S., III; Burns, L.

    1988-01-01

    Describes a simple high-performance liquid chromatography experiment for undergraduate biochemistry laboratories. The experiment illustrates the separation of polypeptides by a step gradient elution using a single pump instrument with no gradient attachments. Discusses instrumentation, analysis, a sample preparation, and results. (CW)

  5. H. W. Laboratory manual: 100 Area section

    Energy Technology Data Exchange (ETDEWEB)

    1950-07-01

    The purpose of this manual is to present a Hazard Breakdown of all jobs normally encountered in the laboratory work of the three sections comprising the Analytic Section, Metallurgy and Control Division of the Technical Department. A Hazard Breakdown is a careful analysis of any job in which the source of possible dangers is clearly indicated for each particular step. The analysis is prepared by individuals who are thoroughly familiar with the specific job or procedure. It is felt that if the hazards herein outlined are recognized by the Laboratory personnel and the suggested safety cautions followed, the chance for injury will be minimized and the worker will become generally more safety conscious. The manual, which is prefaced by the general safety rules applying to all the laboratories, is divided into three main sections, one for each of the three sections into which the Laboratories Division is divided. These sections are as follows: Section 1 -- 200 Area Control; Section 2 -- 100 Area Control; Section 3 -- 300 Area Control, Essential Materials, and Methods Improvement.

  6. Raman Investigation of Temperature Profiles of Phospholipid Dispersions in the Biochemistry Laboratory

    Science.gov (United States)

    Craig, Norman C.

    2015-06-01

    The temperature dependence of self-assembled, cell-like dispersions of phospholipids is investigated with Raman spectroscopy in the biochemistry laboratory. Vibrational modes in the hydrocarbon interiors of phospholipid bilayers are strongly Raman active, whereas the vibrations of the polar head groups and the water matrix have little Raman activity. From Raman spectra increases in fluidity of the hydrocarbon chains can be monitored with intensity changes as a function of temperature in the CH-stretching region. The experiment uses detection of scattered 1064-nm laser light (Nicolet NXR module) by a Fourier transform infrared spectrometer (Nicolet 6700). A thermoelectric heater-cooler device (Melcor) gives convenient temperature control from 5 to 95°C for samples in melting point capillaries. Use of deuterium oxide instead of water as the matrix avoids some absorption of the exciting laser light and interference with intensity observations in the CH-stretching region. Phospholipids studied range from dimyristoylphosphotidyl choline (C14, transition T = 24°C) to dibehenoylphosphotidyl choline (C22, transition T = 74°C).

  7. Improvement of the quality of work in a biochemistry laboratory via measurement system analysis.

    Science.gov (United States)

    Chen, Ming-Shu; Liao, Chen-Mao; Wu, Ming-Hsun; Lin, Chih-Ming

    2016-10-31

    An adequate and continuous monitoring of operational variations can effectively reduce the uncertainty and enhance the quality of laboratory reports. This study applied the evaluation rule of the measurement system analysis (MSA) method to estimate the quality of work conducted in a biochemistry laboratory. Using the gauge repeatability & reproducibility (GR&R) approach, variations in quality control (QC) data among medical technicians in conducting measurements of five biochemical items, namely, serum glucose (GLU), aspartate aminotransferase (AST), uric acid (UA), sodium (Na) and chloride (Cl), were evaluated. The measurements of the five biochemical items showed different levels of variance among the different technicians, with the variances in GLU measurements being higher than those for the other four items. The ratios of precision-to-tolerance (P/T) for Na, Cl and GLU were all above 0.5, implying inadequate gauge capability. The product variation contribution of Na was large (75.45% and 31.24% in normal and abnormal QC levels, respectively), which showed that the impact of insufficient usage of reagents could not be excluded. With regard to reproducibility, high contributions (of more than 30%) of variation for the selected items were found. These high operator variation levels implied that the possibility of inadequate gauge capacity could not be excluded. The ANOVA of GR&R showed that the operator variations in GLU measurements were significant (F=5.296, P=0.001 in the normal level and F=3.399, P=0.015 in the abnormal level, respectively). In addition to operator variations, product variations of Na were also significant for both QC levels. The heterogeneity of variance for the five technicians showed significant differences for the Na and Cl measurements in the normal QC level. The accuracy of QC for five technicians was identified for further operational improvement. This study revealed that MSA can be used to evaluate product and personnel errors and to

  8. Doing That Thing That Scientists Do: A Discovery-Driven Module on Protein Purification and Characterization for the Undergraduate Biochemistry Laboratory Classroom

    Science.gov (United States)

    Garrett, Teresa A.; Osmundson, Joseph; Isaacson, Marisa; Herrera, Jennifer

    2015-01-01

    In traditional introductory biochemistry laboratory classes students learn techniques for protein purification and analysis by following provided, established, step-by-step procedures. Students are exposed to a variety of biochemical techniques but are often not developing procedures or collecting new, original data. In this laboratory module,…

  9. Capillary blood sampling: national recommendations on behalf of the Croatian Society of Medical Biochemistry and Laboratory Medicine.

    Science.gov (United States)

    Krleza, Jasna Lenicek; Dorotic, Adrijana; Grzunov, Ana; Maradin, Miljenka

    2015-01-01

    Capillary blood sampling is a medical procedure aimed at assisting in patient diagnosis, management and treatment, and is increasingly used worldwide, in part because of the increasing availability of point-of-care testing. It is also frequently used to obtain small blood volumes for laboratory testing because it minimizes pain. The capillary blood sampling procedure can influence the quality of the sample as well as the accuracy of test results, highlighting the need for immediate, widespread standardization. A recent nationwide survey of policies and practices related to capillary blood sampling in medical laboratories in Croatia has shown that capillary sampling procedures are not standardized and that only a small proportion of Croatian laboratories comply with guidelines from the Clinical Laboratory Standards Institute (CLSI) or the World Health Organization (WHO). The aim of this document is to provide recommendations for capillary blood sampling. This document has been produced by the Working Group for Capillary Blood Sampling within the Croatian Society of Medical Biochemistry and Laboratory Medicine. Our recommendations are based on existing available standards and recommendations (WHO Best Practices in Phlebotomy, CLSI GP42-A6 and CLSI C46-A2), which have been modified based on local logistical, cultural, legal and regulatory requirements. We hope that these recommendations will be a useful contribution to the standardization of capillary blood sampling in Croatia.

  10. An Undergraduate Investigation into the 10-23 DNA Enzyme that Cleaves RNA: DNA Can Cut It in the Biochemistry Laboratory

    Science.gov (United States)

    Flynn-Charlebois, Amber; Burns, Jamie; Chapelliquen, Stephanie; Sanmartino, Holly

    2011-01-01

    A low-cost biochemistry experiment is described that demonstrates current techniques in the use of catalytic DNA molecules and introduces a nonradioactive, nonfluorescent, inexpensive, fast, and safe method for monitoring these nucleic acid reactions. The laboratory involves the exploration of the 10-23 DNA enzyme as it cleaves a specific RNA…

  11. An Inexpensive, Relatively Green, and Rapid Method to Purify Genomic DNA from "Escherichia Coli": An Experiment for the Undergraduate Biochemistry Laboratory

    Science.gov (United States)

    Sims, Paul A.; Branscum, Katie M.; Kao, Lydia; Keaveny, Virginia R.

    2010-01-01

    A method to purify genomic DNA from "Escherichia coli" is presented. The method is an amalgam of published methods but has been modified and optimized for use in the undergraduate biochemistry laboratory. Specifically, the method uses Tide Free 2x Ultra laundry detergent, which contains unspecified proteases and lipases, "n"-butanol, 2-propanol,…

  12. National Academy of Clinical Biochemistry laboratory medicine practice guidelines for use of tumor markers in testicular, prostate, colorectal, breast, and ovarian cancers

    DEFF Research Database (Denmark)

    Sturgeon, Catharine M; Duffy, Michael J; Stenman, Ulf-Håkan

    2008-01-01

    BACKGROUND: Updated National Academy of Clinical Biochemistry (NACB) Laboratory Medicine Practice Guidelines for the use of tumor markers in the clinic have been developed. METHODS: Published reports relevant to use of tumor markers for 5 cancer sites--testicular, prostate, colorectal, breast...

  13. Synthesis, Characterization, and Secondary Structure Determination of a Silk-Inspired, Self-Assembling Peptide: A Laboratory Exercise for Organic and Biochemistry Courses

    Science.gov (United States)

    Albin, Tyler J.; Fry, Melany M.; Murphy, Amanda R.

    2014-01-01

    This laboratory experiment gives upper-division organic or biochemistry undergraduate students a comprehensive look at the synthesis, chemical characterization, self-assembly, and secondary structure determination of small, N-acylated peptides inspired by the protein structure of silkworm silk. All experiments can be completed in one 4 h lab…

  14. Evaluation of an Online Instructional Database Accessed by QR Codes to Support Biochemistry Practical Laboratory Classes

    Science.gov (United States)

    Yip, Tor; Melling, Louise; Shaw, Kirsty J.

    2016-01-01

    An online instructional database containing information on commonly used pieces of laboratory equipment was created. In order to make the database highly accessible and to promote its use, QR codes were utilized. The instructional materials were available anytime and accessed using QR codes located on the equipment itself and within undergraduate…

  15. Analysis of a p53 Mutation Associated with Cancer Susceptibility for Biochemistry and Genetic Laboratory Courses

    Science.gov (United States)

    Soto-Cruz, Isabel; Legorreta-Herrera, Martha

    2009-01-01

    We have devised and implemented a module for an upper division undergraduate laboratory based on the amplification and analysis of a p53 polymorphism associated with cancer susceptibility. First, students collected a drop of peripheral blood cells using a sterile sting and then used FTA cards to extract the genomic DNA. The p53 region is then PCR…

  16. Green, Enzymatic Syntheses of Divanillin and Diapocynin for the Organic, Biochemistry, or Advanced General Chemistry Laboratory

    Science.gov (United States)

    Nishimura, Rachel T.; Giammanco, Chiara H.; Vosburg, David A.

    2010-01-01

    Environmentally benign chemistry is an increasingly important topic both in the classroom and the laboratory. In this experiment, students synthesize divanillin from vanillin or diapocynin from apocynin, using horseradish peroxidase and hydrogen peroxide in water. The dimerized products form rapidly at ambient temperature and are isolated by…

  17. ILIS--An integrated laboratory information system. I. Biochemistry and hematology.

    Science.gov (United States)

    Deshpande, S U

    1982-02-01

    IBM System 34 (central processing unit, 128 kilobytes; fixed disks, 128.4 megabytes) with seven cathode-ray tubes has been used by our clinical laboratories for the last 30 months. All data-entry programs are in a conversational mode, for on-line corrections of possible errors in patient identification and results. Daily reports are removed from the medical records after temporary and permanent cumulative weekly reports are received, which keep a three-month track of the results. The main advantages of the system are: (a) the increasing laboratory work load can be handled with the same staff; (b) the volume of the medical record files on the patients is decreased; (c) an easily retrievable large data base of results is formed for research purposes; (d) faster billing; and (e) the computer system is run without engaging any additional staff.

  18. Overview. Health Physics Laboratory. Section 10

    Energy Technology Data Exchange (ETDEWEB)

    Waligorski, M.P.R. [Institute of Nuclear Physics, Cracow (Poland)

    1995-12-31

    The activities of the Health Physics Laboratory at the Niewodniczanski Institute of Nuclear Physics are presented and namely: research in the area of radiation physics and radiation protection of the employees of the Institute of Nuclear Physics, theoretical research concerns radiation detectors, radiation protection and studies of concepts of radiation protection and experimental research concerns solid state dosimetry. In this report, apart of the detail descriptions of mentioned activities, the information about personnel employed in the Department, papers and reports published in 1994, contribution to conferences and grants are also given.

  19. Environmental regulation of plant gene expression: an RT-qPCR laboratory project for an upper-level undergraduate biochemistry or molecular biology course.

    Science.gov (United States)

    Eickelberg, Garrett J; Fisher, Alison J

    2013-01-01

    We present a novel laboratory project employing "real-time" RT-qPCR to measure the effect of environment on the expression of the FLOWERING LOCUS C gene, a key regulator of floral timing in Arabidopsis thaliana plants. The project requires four 3-hr laboratory sessions and is aimed at upper-level undergraduate students in biochemistry or molecular biology courses. The project provides students with hands-on experience with RT-qPCR, the current "gold standard" for gene expression analysis, including detailed data analysis using the common 2-ΔΔCT method. Moreover, it provides a convenient starting point for many inquiry-driven projects addressing diverse questions concerning ecological biochemistry, naturally occurring genetic variation, developmental biology, and the regulation of gene expression in nature.

  20. Comparison of the microbial dynamics and biochemistry of laboratory sourdoughs prepared with grape, apple and yogurt.

    Science.gov (United States)

    Gordún, Elena; del Valle, Luis J; Ginovart, Marta; Carbó, Rosa

    2015-09-01

    The microbiological culture-dependent characterization and physicochemical characteristics of laboratory sourdough prepared with grape (GS) were evaluated and compared with apple (AS) and yogurt (YS), which are the usual Spanish sourdough ingredients. Ripe GS took longer than AS and YS to reach the appropriate acidity and achieved lower values of lactic acid. In all sourdoughs, the lactic acid bacteria (LAB) increased during processing and were the dominant microorganisms (>1E+8 CFU/g). GS, as well as AS, had high diversity of LAB species. In ripe YS, Pediococcus pentosaceus was the only species identified; in GS and AS, several Lactobacilli were also found, Lb. plantarum, Lb. brevis, and Lb. sakei; in addition, in GS Weisella cibaria also appeared. Regarding the yeast population, non-Saccharomyces yeasts from GS and AS showed a very high specific population (>1E+7 CFU/g), but this was reduced in ripe sourdough (Saccharomyces group dominated in all sourdoughs. Starting ingredients or raw material provided microbiological specificity to sourdoughs, and grape could be considered one of them.

  1. Current practice in laboratory diagnostics of autoimmune diseases in Croatia. 
Survey of the Working group for laboratory diagnostics of autoimmune diseases of the Croatian Society of Medical Biochemistry and Laboratory Medicine

    Science.gov (United States)

    Kuna, Andrea Tešija; Đerek, Lovorka; Kozmar, Ana; Drvar, Vedrana

    2016-01-01

    Introduction With the trend of increasing incidence of autoimmune diseases, laboratories are faced with exponential growth of the requests for tests relating the diagnosis of these diseases. Unfortunately, the lack of laboratory personnel experienced in this specific discipline of laboratory diagnostic, as well as an unawareness of a method limitation often results in confusion for clinicians. The aim was to gain insight into number and type of Croatian laboratories that perform humoral diagnostics with the final goal to improve and harmonize laboratory diagnostics of autoimmune diseases in Croatia. Materials and methods In order to get insight into current laboratory practice two questionnaires, consisting of 42 questions in total, were created. Surveys were conducted using SurveyMonkey application and were sent to 88 medical biochemistry laboratories in Croatia for the first survey. Out of 33 laboratories that declared to perform diagnostic from the scope, 19 were selected for the second survey based on the tests they pleaded to perform. The survey comprised questions regarding autoantibody hallmarks of systemic autoimmune diseases while regarding organ-specific autoimmune diseases was limited to diseases of liver, gastrointestinal and nervous system. Results Response rate was high with 80 / 88 (91%) laboratories which answered the first questionnaire, and 19 / 19 (1.0) for the second questionnaire. Obtained results of surveys indicate high heterogeneity in the performance of autoantibody testing among laboratories in Croatia. Conclusions Results indicate the need of creating recommendations and algorithms in order to harmonize the approach to laboratory diagnostics of autoimmune diseases in Croatia.

  2. Biochemistry and metabolism of lake trout: laboratory and field studies on the effects of contaminants

    Science.gov (United States)

    Passino, Dora R. May

    1981-01-01

    To evaluate the effects of ambient and higher concentrations of PCB's (Aroclor 1254) and DDE in food and water on fry of lake trout (Salvelinus namaycush) from Lake Michigan, I measured several biochemical indicators of stress in exposed and unexposed (control) fry. No differences between treatments were observed in oxygen consumption rates or lactate concentrations of unexercised fry, but apparent differences in specific swimming speed and lactate response in fry that swam to exhaustion suggested that exposed fry had lower stamina. Observed differences between biochemical profiles of 1-day-old sac fry reared from eggs originating from lake trout collected off Saugatuck and those originating from eggs of brood stock at the Marquette (Michigan) hatchery may have been caused by organochlorine contamination or by genetic and dietary differences between the parental stocks. Activity of the enzyme allantoinase was measured in juvenile and adult lake trout as an indicator of sublethal effects of Great Lakes contaminants. The 50% inhibition of allantoinase in vitro occurred at 6.0 mg/L Cu++, 6.7 mg/L Cd++, 34 mg/L Hg++, and 52 mg/L Pb++. Allantoinase was not affected by in vitro exposure to PCB's up to 7 μg/g, or DDE or DDT up to 10 μg/g; however, in vivo exposure resulting in 2.6 μg/g PCB's in the whole fish activated allantoinase slightly (10% significance level). Allantoinase activity was negatively correlated with total length for fish from Lake Michigan but not for fish from Lake Superior or from laboratory stocks. Mercury, PCB's, and DDT, possibly acting in combination with each other and with additional contaminants, may be the cause of the negative correlation of allantoinase activity with size in Lake Michigan lake trout.

  3. Doing that thing that scientists do: A discovery-driven module on protein purification and characterization for the undergraduate biochemistry laboratory classroom.

    Science.gov (United States)

    Garrett, Teresa A; Osmundson, Joseph; Isaacson, Marisa; Herrera, Jennifer

    2015-01-01

    In traditional introductory biochemistry laboratory classes students learn techniques for protein purification and analysis by following provided, established, step-by-step procedures. Students are exposed to a variety of biochemical techniques but are often not developing procedures or collecting new, original data. In this laboratory module, students develop research skills through work on an original research project and gain confidence in their ability to design and execute an experiment while faculty can enhance their scholarly pursuits through the acquisition of original data in the classroom laboratory. Students are prepared for a 6-8 week discovery-driven project on the purification of the Escherichia coli cytidylate kinase (CMP kinase) through in class problems and other laboratory exercises on bioinformatics and protein structure analysis. After a minimal amount of guidance on how to perform the CMP kinase in vitro enzyme assay, SDS-PAGE, and the basics of protein purification, students, working in groups of three to four, develop a protein purification protocol based on the scientific literature and investigate some aspect of CMP kinase that interests them. Through this process, students learn how to implement a new but perhaps previously worked out procedure to answer their research question. In addition, they learn the importance of keeping a clear and thorough laboratory notebook and how to interpret their data and use that data to inform the next set of experiments. Following this module, students had increased confidence in their ability to do basic biochemistry techniques and reported that the "self-directed" nature of this lab increased their engagement in the project.

  4. Concept maps as evaluation tools of the integrative construction level of meanings in the biochemistry laboratory under a constructivist approach

    Directory of Open Access Journals (Sweden)

    Julia Flores

    2014-12-01

    Full Text Available This descriptive-comparative work was carried out with 13 students in the areas of Biology and Chemistry of the Pedagogical Institute of Caracas in a course of Biochemistry, who formed two lab groups. Its purpose was to compare the level of integrative construction of scientific meanings achieved in the concept maps developed in an integrated manner to the V diagram around a problem situation raised on enzymes, considering previous knowledge about this disciplinary content. Two statistics were used to compare the groups: student's t test for the pre-existing knowledge and Mann-Whitney U for concept maps. A General Index of Integrative Construction of meanings (IGCIS proposed by the authors, was incorporated in a novel way to assess concept maps. The results revealed the discriminatory power of IGCIS, being able to differentiate the groups in a more consistent way and in accordance with their levels of prior knowledge.

  5. Nutritional Biochemistry

    Science.gov (United States)

    Smith, Scott M.

    2010-01-01

    This slide presentation reviews some of the effects that space flight has on humans nutritional biochemistry. Particular attention is devoted to the study of protein breakdown, inflammation, hypercatabolism, omega 3 fatty acids, vitamin D, calcium, urine, folate and nutrient stability of certain vitamins, the fluid shift and renal stone risk, acidosis, iron/hematology, and the effects on bone of dietary protein, potassium. inflammation, and omega-3 fatty acids

  6. Prepare, Do, Review: A Skills-Based Approach for Laboratory Practical Classes in Biochemistry and Molecular Biology

    Science.gov (United States)

    Arthur, Peter; Ludwig, Martha; Castelli, Joane; Kirkwood, Paul; Attwood, Paul

    2016-01-01

    A new laboratory practical system is described which is comprised of a number of laboratory practical modules, each based around a particular technique or set of techniques, related to the theory part of the course but not designed to be dependent on it. Each module comprises an online recorded pre-lab lecture, the laboratory practical itself and…

  7. Postmortem biochemistry

    Directory of Open Access Journals (Sweden)

    Mukaddes Gürler

    2014-12-01

    Full Text Available Postmortem biochemistry is becoming more important in forensic pathology. Involving of biochemical investigations full autopsy can provide to detect divers pathologic conditions such as antemortem acute/chronic diseases, fatal metabolic conditions, survival time, postmortem biochemical changes, and the source of analytes. Biochemical tests may be usefull where the morphological examinations cannot lighten the fatal pathology (Diabetes Mellitus (DM, alcoholic ketoacidosis, sepsis, electrolytic disorders. This article presents the analytes that may be useful in forensic medicine upon the studies performed and published in the literature.

  8. Expression, purification, and characterization of a carbohydrate-active enzyme: A research-inspired methods optimization experiment for the biochemistry laboratory.

    Science.gov (United States)

    Willbur, Jaime F; Vail, Justin D; Mitchell, Lindsey N; Jakeman, David L; Timmons, Shannon C

    2016-01-01

    The development and implementation of research-inspired, discovery-based experiences into science laboratory curricula is a proven strategy for increasing student engagement and ownership of experiments. In the novel laboratory module described herein, students learn to express, purify, and characterize a carbohydrate-active enzyme using modern techniques and instrumentation commonly found in a research laboratory. Unlike in a traditional cookbook-style experiment, students generate their own hypotheses regarding expression conditions and quantify the amount of protein isolated using their selected variables. Over the course of three 3-hour laboratory periods, students learn to use sterile technique to express a protein using recombinant DNA in E. coli, purify the resulting enzyme via affinity chromatography and dialysis, analyze the success of their purification scheme via SDS-PAGE, assess the activity of the enzyme via an HPLC-based assay, and quantify the amount of protein isolated via a Bradford assay. Following the completion of this experiment, students were asked to evaluate their experience via an optional survey. All students strongly agreed that this laboratory module was more interesting to them than traditional experiments because of its lack of a pre-determined outcome and desired additional opportunities to participate in the experimental design process. This experiment serves as an example of how research-inspired, discovery-based experiences can benefit both the students and instructor; students learned important skills necessary for real-world biochemistry research and a more concrete understanding of the research process, while generating new knowledge to enhance the scholarly endeavors of the instructor.

  9. An inquiry-based biochemistry laboratory structure emphasizing competency in the scientific process: a guided approach with an electronic notebook format.

    Science.gov (United States)

    L Hall, Mona; Vardar-Ulu, Didem

    2014-01-01

    The laboratory setting is an exciting and gratifying place to teach because you can actively engage the students in the learning process through hands-on activities; it is a dynamic environment amenable to collaborative work, critical thinking, problem-solving and discovery. The guided inquiry-based approach described here guides the students through their laboratory work at a steady pace that encourages them to focus on quality observations, careful data collection and thought processes surrounding the chemistry involved. It motivates students to work in a collaborative manner with frequent opportunities for feedback, reflection, and modification of their ideas. Each laboratory activity has four stages to keep the students' efforts on track: pre-lab work, an in-lab discussion, in-lab work, and a post-lab assignment. Students are guided at each stage by an instructor created template that directs their learning while giving them the opportunity and flexibility to explore new information, ideas, and questions. These templates are easily transferred into an electronic journal (termed the E-notebook) and form the basic structural framework of the final lab reports the students submit electronically, via a learning management system. The guided-inquiry based approach presented here uses a single laboratory activity for undergraduate Introductory Biochemistry as an example. After implementation of this guided learning approach student surveys reported a higher level of course satisfaction and there was a statistically significant improvement in the quality of the student work. Therefore we firmly believe the described format to be highly effective in promoting student learning and engagement.

  10. Application of sigma metrics for the assessment of quality assurance in clinical biochemistry laboratory in India: a pilot study.

    Science.gov (United States)

    Singh, Bhawna; Goswami, Binita; Gupta, Vinod Kumar; Chawla, Ranjna; Mallika, Venkatesan

    2011-04-01

    Ensuring quality of laboratory services is the need of the hour in the field of health care. Keeping in mind the revolution ushered by six sigma concept in corporate world, health care sector may reap the benefits of the same. Six sigma provides a general methodology to describe performance on sigma scale. We aimed to gauge our laboratory performance by sigma metrics. Internal quality control (QC) data was analyzed retrospectively over a period of 6 months from July 2009 to December 2009. Laboratory mean, standard deviation and coefficient of variation were calculated for all the parameters. Sigma was calculated for both the levels of internal QC. Satisfactory sigma values (>6) were elicited for creatinine, triglycerides, SGOT, CPK-Total and Amylase. Blood urea performed poorly on the sigma scale with sigma six sigma standards for all the analytical processes.

  11. Effects of Various Dental Materials on Alkaline Phosphatase Extracted from Pulp: An Experiment for the Biochemistry Laboratory.

    Science.gov (United States)

    Thompson, Lorin R.

    1980-01-01

    A laboratory experiment that demonstrates the effects of various dental materials on a representative enzyme from the pulp is outlined. The experiment encourages students to consider the effects that various restorative materials and techniques might have on enzymes in the living pulp. (Author/MLW)

  12. DNA Fingerprint Analysis of Three Short Tandem Repeat (STR) Loci for Biochemistry and Forensic Science Laboratory Courses

    Science.gov (United States)

    McNamara-Schroeder, Kathleen; Olonan, Cheryl; Chu, Simon; Montoya, Maria C.; Alviri, Mahta; Ginty, Shannon; Love, John J.

    2006-01-01

    We have devised and implemented a DNA fingerprinting module for an upper division undergraduate laboratory based on the amplification and analysis of three of the 13 short tandem repeat loci that are required by the Federal Bureau of Investigation Combined DNA Index System (FBI CODIS) data base. Students first collect human epithelial (cheek)…

  13. Detection of an ABCA1 Variant Associated with Type 2 Diabetes Mellitus Susceptibility for Biochemistry and Genetic Laboratory Courses

    Science.gov (United States)

    Legorreta-Herrera, M.; Mosqueda-Romo, N. A.; Hernández-Clemente, F.; Soto-Cruz, I.

    2013-01-01

    We selected diabetes mellitus for this laboratory exercise to provide students with an explicit model for scientific research concerning the association between the R230C polymorphism and susceptibility to type 2 diabetes mellitus, which is highly prevalent in the Mexican population. We used a collaborative project-based learning to engage…

  14. Design and Calibration of the US Army Research Laboratory (ARL) Closed Loop Laboratory Radio Frequency (RF) Propagation Section

    Science.gov (United States)

    2016-10-01

    Loop Laboratory radio frequency (RF) section, along with the commercially available absorber and horn antennas used in constructing the RF section...Laboratory Radio Frequency (RF) Propagation Section by William Coburn, Seth A McCormick, and Andre F Witcher Approved for... Radio Frequency (RF) Propagation Section by William Coburn, Seth A McCormick, and Andre F Witcher Sensors and Electron Devices Directorate, ARL

  15. Biochemistry and Molecular Biology Education (BAMBED

    Directory of Open Access Journals (Sweden)

    Voet Donald

    2004-05-01

    Full Text Available Biochemistry and Molecular Biology Education (BAMBED is a journal that is a publication of the In-ternational Union of Biochemistry and Molecular Biology (IUBMB and is published by the AmericanSociety of Biochemistry and Molecular Biology (ASBMB. BAMBED, as its name indicates, publishesarticles of interest to educators in biochemistry and molecular biology. These include invited reviewson subjects not yet in textbooks, discussions of curricular development, new laboratory exercises,and articles on educational research. BAMBED also publishes Features on Problem-Based Learning(PBL, Biotechnology Education, and Multimedia in Biochemistry and Molecular Biology Educati-on. An important aspect of these articles is that their educational eectiveness must be assessed. Ishall discuss in greater detail the types of articles that BAMBED publishes and the criteria used foraccepting them for publication. Conference attendees are encouraged to submit articles to BAMBED.

  16. Bringing the Excitement and Motivation of Research to Students; Using Inquiry and Research-Based Learning in a Year-Long Biochemistry Laboratory: Part II--Research-Based Laboratory--A Semester-Long Research Approach Using Malate Dehydrogenase as a Research Model

    Science.gov (United States)

    Knutson, Kristopher; Smith, Jennifer; Nichols, Paul; Wallert, Mark A.; Provost, Joseph J.

    2010-01-01

    Research-based learning in a teaching environment is an effective way to help bring the excitement and experience of independent bench research to a large number of students. The program described here is the second of a two-semester biochemistry laboratory series. Here, students are empowered to design, execute and analyze their own experiments…

  17. Clinical biochemistry

    Science.gov (United States)

    Alexander, W. C.; Leach, C. S.; Fischer, C. L.

    1975-01-01

    The objectives of the biochemical studies conducted for the Apollo program were (1) to provide routine laboratory data for assessment of preflight crew physical status and for postflight comparisons; (2) to detect clinical or pathological abnormalities which might have required remedial action preflight; (3) to discover as early as possible any infectious disease process during the postflight quarantine periods following certain missions; and (4) to obtain fundamental medical knowledge relative to man's adjustment to and return from the space flight environment. The accumulated data presented suggest that these requirements were met by the program described. All changes ascribed to the space flight environment were subtle, whereas clinically significant changes were consistent with infrequent illnesses unrelated to the space flight exposure.

  18. Incremental Sampling Methodology (ISM). Part 2, Section 6: Laboratory Processing

    Science.gov (United States)

    2012-03-01

    Pick the wrong option • Poor and unknown bias 4 Include Lab Processing in Project Planning Lab ITRC, ISM-1, Section 6.1.1 5 Define the Analytes...Disaggregation and sieving • Nugget effect expected to be small  Contaminant exposure sprayed as a liquid  Mill • Puck mill  Comparison study planned 16 Lab...Subsampling 22 Dry Splitting Options  Rotary sectorial splitter ITRC, ISM-1, Section 6.2.2.7 23 Subsampling Options  2-Dimensional Japanese Slabcake

  19. Learning Biochemistry by Chocolate

    Directory of Open Access Journals (Sweden)

    M.C Guedes

    2006-07-01

    Full Text Available Both sensations and biochemical reactions taken place or promoted during ingestion of chocolate were the motivation for  investigating  the  organic  compounds  present  in  this  source.  Cocoa  and  chocolate  are  composed  by  several substances , among them, aminoacids and alkaloids.The objective of this investigation was to purpose a contextured approach  of  biochemistry  through  the  sensations  and  reactions  involving  aminoacids,  theobromine  and  hormones. Methodology: 1. Theoretical part:  constituted  by theoretical  and tutorial classes  about aminoacids, theobromine and hormones  involved  at  the  metabolism;  2.  Questionary:  ten  questions  based  upon  theoretical  classes,  personal sensations  and  general  aspects  of chocolate;  3.Lecture:  Cientific  articles  searched  in  periodics  by  own  students  as well  as  newspaper  reports;  4.  Experimental:  Laboratory  experiments  including  extraction,  characterization, spectrometric quantification  after  specific reactions  and identification by  Rf  comparison with  standards  on TLC  from cocoa  almonds  and  both  powder  cocoa  and  chocolate.  The  study  was  applied  in  30  students  from  a  chemistry college. Results: The results pointed out to a higher frequency of the students and to a increased interest  from them by   biochemistry  issues  and  cientific  lectures,  as  well  as  a  satisfactory  acquirement  of  theoretical  and  practice knowledge of aminoacids and hormones, spectrometry and chromatography. Conclusion: A contextured approach is quite positive for learning biochemistry to chemists.

  20. 76 FR 39110 - Medicare Program; Section 3113: The Treatment of Certain Complex Diagnostic Laboratory Tests...

    Science.gov (United States)

    2011-07-05

    ... classified (NOC)'' code but that would otherwise meet the criteria set forth in section 3113 for being a... Procedure Coding System (HCPCS) code other than a not otherwise classified (NOC) code under such Coding... for diagnostic laboratory tests defined in section 3113(a)(2) but currently billed using NOC...

  1. A Streamlined Molecular Biology Module for Undergraduate Biochemistry Labs

    Science.gov (United States)

    Muth, Gregory W.; Chihade, Joseph W.

    2008-01-01

    Site-directed mutagenesis and other molecular biology techniques, including plasmid manipulation and restriction analysis, are commonly used tools in the biochemistry research laboratory. In redesigning our biochemistry lab curricula, we sought to integrate these techniques into a term-long, project-based course. In the module presented here,…

  2. Reproduction, physiology and biochemistry

    Science.gov (United States)

    This chapter summarizes fundamental knowledge and recent discoveries about the reproduction, physiology and biochemistry of plant-parasitic nematodes. Various types of reproduction are reviewed, including sexual reproduction and mitotic and meiotic parthenogenesis. Although much is known about the p...

  3. Bringing the Excitement and Motivation of Research to Students; Using Inquiry and Research-Based Learning in a Year-Long Biochemistry Laboratory: Part I--Guided Inquiry--Purification and Characterization of a Fusion Protein--Histidine Tag, Malate Dehydrogenase, and Green Fluorescent Protein

    Science.gov (United States)

    Knutson, Kristopher; Smith, Jennifer; Wallert, Mark A.; Provost, Joseph J.

    2010-01-01

    A successful laboratory experience provides the foundation for student success, creating active participation in the learning process. Here, we describe a new approach that emphasizes research, inquiry and problem solving in a year-long biochemistry experience. The first semester centers on the purification, characterization, and analysis of a…

  4. Expression, Purification, and Characterization of a Carbohydrate-Active Enzyme: A Research-Inspired Methods Optimization Experiment for the Biochemistry Laboratory

    Science.gov (United States)

    Willbur, Jaime F.; Vail, Justin D.; Mitchell, Lindsey N.; Jakeman, David L.; Timmons, Shannon C.

    2016-01-01

    The development and implementation of research-inspired, discovery-based experiences into science laboratory curricula is a proven strategy for increasing student engagement and ownership of experiments. In the novel laboratory module described herein, students learn to express, purify, and characterize a carbohydrate-active enzyme using modern…

  5. Environmental Regulation of Plant Gene Expression: An Rt-qPCR Laboratory Project for an Upper-Level Undergraduate Biochemistry or Molecular Biology Course

    Science.gov (United States)

    Eickelberg, Garrett J.; Fisher, Alison J.

    2013-01-01

    We present a novel laboratory project employing "real-time" RT-qPCR to measure the effect of environment on the expression of the "FLOWERING LOCUS C" gene, a key regulator of floral timing in "Arabidopsis thaliana" plants. The project requires four 3-hr laboratory sessions and is aimed at upper-level undergraduate…

  6. Biochemistry (by Jochanan Stenesh)

    Science.gov (United States)

    Glasfeld, Arthur

    1999-06-01

    Plenum: New York, 1998. Hardcover, ISBN 0 306-45732-6. 95. Paperback, ISBN 0 306 45733 4. 55 (set of 3). Solutions manual and transparencies available. According to the promotional materials accompanying this text, its intended audience is students in one-semester undergraduate biochemistry courses. At just over 500 pages, the book is shorter than the norm of well over 1000 pages. The challenge, then, is to present the subject in a coherent and compelling fashion while necessarily omitting a large fraction of the material that one normally finds in more inclusive texts. That kind of editing is obviously going to lead to squawking from some quarters, so I should put my prejudices on the table. I teach a one-semester course in biochemical structure, and I have a long-standing interest in using molecular models to explain biochemical behavior, both in research and in teaching. The editing performed by Professor Stenesh is likely to trouble someone with a structural or mechanistic background. Rather than selectively excluding some topics, Stenesh has created a table of contents that looks like it's from a much longer text. The usual chapters on biochemical structure, catalysis, metabolism and molecular genetics are included here. The ax fell elsewhere, and most obvious to my eye are the omissions of structure and chemical mechanism beyond those few chapters that are dedicated to them. A brief presentation on the structure and function of hemoglobin is given in the chapter on proteins, and the catalytic mechanism of chymotrypsin is briefly presented in the chapter on catalysis. But in chapters on metabolism, the structures of substrates and products are shown while mechanisms of conversion are omitted. For example, in the description of aldolase, we're informed that the enzyme catalyzes a reverse aldol condensation, but the reader isn't shown how the aldol condensation relates to the chemical conversion we see in the figure. (Part of the problem may be that the text

  7. BOREAS TE-9 NSA Canopy Biochemistry

    Science.gov (United States)

    Hall, Forrest G. (Editor); Curd, Shelaine (Editor); Margolis, Hank; Charest, Martin; Sy, Mikailou

    2000-01-01

    The BOREAS TE-9 team collected several data sets related to chemical and photosynthetic properties of leaves. This data set contains canopy biochemistry data collected in 1994 in the NSA at the YJP, OJR, OBS, UBS, and OA sites, including biochemistry lignin, nitrogen, cellulose, starch, and fiber concentrations. These data were collected to study the spatial and temporal changes in the canopy biochemistry of boreal forest cover types and how a high-resolution radiative transfer model in the mid-infrared could be applied in an effort to obtain better estimates of canopy biochemical properties using remote sensing. The data are available in tabular ASCII files. The data files are available on a CD-ROM (see document number 20010000884), or from the Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC).

  8. [superscript 1]H NMR Spectroscopy-Based Configurational Analysis of Mono- and Disaccharides and Detection of ß-Glucosidase Activity: An Undergraduate Biochemistry Laboratory

    Science.gov (United States)

    Periyannan, Gopal R.; Lawrence, Barbara A.; Egan, Annie E.

    2015-01-01

    A [superscript 1]H NMR spectroscopy-based laboratory experiment explores mono- and disaccharide structural chemistry, and the enzyme-substrate specificity of glycosidic bond cleavage by ß-glucosidase towards cellobiose (ß-linked gluco-disaccharide) and maltose (a-linked gluco-disaccharide). Structural differences between cellobiose, maltose, and…

  9. Biochemical Neuroscience Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — This biochemistry lab is set up for protein analysis using Western blot, enzyme linked immunosorbent assays, immunohistochemistry, and bead-based immunoassays. The...

  10. Environmental biochemistry of current environmental levels of heavy metals: preparation of radiotracers with very high specific radioactivity for metallobiochemical experiments on laboratory animals.

    Science.gov (United States)

    Sabbioni, E; Goetz, L; Birattari, C; Bonardi, M

    1981-03-01

    Environmental toxicology research on dose-response relationships of heavy metals requires experiments on laboratory animals exposed to "low doses" of trace elements which should reflect "present or actual environmental levels" characteristic of polluted environments. Unfortunately no criteria exist to establish the "low doses" to which laboratory animals must be exposed, in practice the choice of the level used is made in an almost arbitrary manner. In order to define the "present environmental levels" of heavy metals which should be administered to laboratory animals an approach is suggested, based upon knowledge of the concentrations of trace elements in the diet, air and food as well as the fractions absorbed. Today daily intakes of trace elements by man are of the order of few micrograms or nanograms thus requiring the use of extremely sensitive analytical techniques to determine the very low amounts of heavy metals in tissues and cellular components. In these fields of research the use of radiotracers with very high specific radioactivity appears particularly advantageous but requires considerable care during their preparation and use. The first part of this paper deals with a definition of the ranges of concentrations of trace elements which should be used for metabolic studies on laboratory animals when they are exposed via different routes such as ingestion, inhalation in injection; the second part describes the production of radiotracers with very high specific radioactivity by proton activation in the cyclotron and by neutron irradiation in the nuclear reactor. Their use to label present levels of heavy metals under conditions adapted for biochemical purposes, as well as the preparation of different metal-labelled chemical species is also reported. Particular attention is directed to quality control of the radiotracer solutions which are administered to the animals including those of radioactivity concentrations, radioisotopic purity, radiochemical purity

  11. Laboratory Measurements and Modeling of Molecular Photoabsorption Cross Sections in the Ultraviolet for Astronomical Applications

    Science.gov (United States)

    Stark, Glenn; Smith, P. L.

    2008-09-01

    Our research program is dedicated to the measurement and modeling of vacuum ultraviolet and UV molecular photoabsorption cross sections with the highest practical resolution. It supports efforts to interpret and model observations of planetary atmospheres. Measurement and modeling efforts on molecular nitrogen, carbon dioxide, sulfur dioxide, and diatomic sulfur are in progress. N2: We focus on the measurement of line f-values and line widths within the complex spectrum between 80 and 100 nm. Our measurements are incorporated into a theoretical model of the absorption spectrum of N2 which has established the mechanisms responsible for predissociation and reproduces all observed features in 14N2 and its isotopomers as a function of temperature. CO2: The photodissociation of CO2 is a fundamental photochemical process in the atmospheres of Mars and Venus. Our research centers on the measurement of high resolution cross sections from 91 to 120 nm. We have completed measurements at 295 K and 195 K over the 106 to 120 nm region, and we are analyzing recent measurements of cross sections between 91 and 106 nm. SO2: We provide astronomers with high-resolution cross section data for the complex ultraviolet absorption spectrum between 198 and 325 nm. Using the Imperial College VUV Fourier transform spectrometer, we have completed and published room temperature cross sections, and we are completing work on low-temperature measurements over the same spectral region. S2: Interpretations of atmospheric (Io, Jupiter, cometary comae) S2 absorption features are hindered by a complete lack of laboratory cross section data in the ultraviolet. We are beginning an effort to quantify the photoabsorption spectrum of S2 from 200 to 300 nm based on theoretical calculations and laboratory measurements. Coupled-channel calculations will be complemented by measurements of the absorption spectrum of S2 at high resolution.

  12. Laboratory Measurements and Modeling of Molecular Photoabsorption Cross Sections for Planetary Applications

    Science.gov (United States)

    Stark, G.; Smith, P. L.; Lewis, B. R.; Heays, A.; Blackie, D.; Pickering, J.

    2010-12-01

    Our research program comprises the measurement and modeling of ultraviolet molecular photoabsorption cross sections with the highest practical resolution. It supports efforts to interpret and model observations of planetary atmospheres. Measurement and modeling efforts on molecular nitrogen, sulfur dioxide, diatomic sulfur, and carbon dioxide are in progress. Molecular Nitrogen: We focus on the measurement of line f-values and line widths within the complex spectrum between 80 and 100 nm. Our measurements are incorporated into a theoretical model of the absorption spectrum of N2 which has established the mechanisms responsible for predissociation and reproduces all observed features in 14N2 and its isotopomers as a function of temperature. Sulfur Dioxide: We provide astronomers with high-resolution cross section data for the complex ultraviolet SO2 absorption spectrum. Using the Imperial College VUV Fourier transform spectrometer, we have completed and published room temperature cross sections from 198 to 325 nm and 160 K cross sections from 199 to 220 nm. We have completed work on further low-temperature measurements from 220 to 325 nm. Diatomic Sulfur: Interpretations of atmospheric (Io, Jupiter, cometary comae) S2 absorption features are hindered by a complete lack of laboratory cross section data in the ultraviolet. We are working to quantify the photoabsorption spectrum of S2 from 200 to 300 nm based on laboratory measurements and theoretical calculations. We have designed an experimental apparatus to produce a stable column of S2 vapor. Measurements of the absorption spectrum of S2 at high resolution will be complemented by coupled-channel calculations. Carbon Dioxide: The photodissociation of CO2 is a fundamental photochemical process in the atmospheres of Mars and Venus. Our research centers on the measurement of high resolution cross sections from 87 to 120 nm. We have completed measurements at 295 K and 195 K over the 106 to 120 nm region, and we have

  13. Laboratory Measurements and Modeling of Molecular Photoabsorption Cross Sections in the Ultraviolet for Planetary Atmospheres Applications

    Science.gov (United States)

    Stark, Glenn; Smith, P. L.; Lewis, B. R.; Heays, A. N.; Pickering, J.; Blackwell-Whitehead, R.; Blackie, D.

    2008-09-01

    Our research program is dedicated to the measurement and modeling of vacuum ultraviolet and UV molecular photoabsorption cross sections with the highest practical resolution. It supports efforts to interpret and model observations of planetary atmospheres. Measurement and modeling efforts on molecular nitrogen, carbon dioxide, sulfur dioxide, and diatomic sulfur are in progress. N2: We focus on the measurement of line f-values and line widths within the complex spectrum between 80 and 100 nm. Our measurements are incorporated into a theoretical model of the absorption spectrum of N2 which has established the mechanisms responsible for predissociation and reproduces all observed features in 14N2 and its isotopomers as a function of temperature. CO2: The photodissociation of CO2 is a fundamental photochemical process in the atmospheres of Mars and Venus. Our research centers on the measurement of high resolution cross sections from 91 to 120 nm. We have completed measurements at 295 K and 195 K over the 106 to 120 nm region, and we are analyzing recent measurements of cross sections between 91 and 106 nm. SO2: We provide astronomers with high-resolution cross section data for the complex ultraviolet absorption spectrum between 198 and 325 nm. Using the Imperial College VUV Fourier transform spectrometer, we have completed and published room temperature cross sections, and we are completing work on low-temperature measurements over the same spectral region. S2: Interpretations of atmospheric (Io, Jupiter, cometary comae) S2 absorption features are hindered by a complete lack of laboratory cross section data in the ultraviolet. We are beginning an effort to quantify the photoabsorption spectrum of S2 from 200 to 300 nm based on theoretical calculations and laboratory measurements. Coupled-channel calculations will be complemented by measurements of the absorption spectrum of S2 at high resolution.

  14. The biochemistry of memory.

    Science.gov (United States)

    Stock, Jeffry B; Zhang, Sherry

    2013-09-09

    Almost fifty years ago, Julius Adler initiated a program of research to gain insights into the basic biochemistry of intelligent behavior by studying the molecular mechanisms that underlie the chemotactic responses of Escherichia coli. All living organisms share elements of a common biochemistry for metabolism, growth and heredity - why not intelligence? Neurobiologists have demonstrated that this is the case for nervous systems in animals ranging from worms to man. Motile unicellular organisms such as E. coli exhibit rudimentary behaviors that can be loosely described in terms of cognitive phenomena such as memory and learning. Adler's initiative at least raised the prospect that, because of the numerous experimental advantages provided by E. coli, it would be the first organism whose behavior could be understood at molecular resolution.

  15. The Importance of a Laboratory Section on Student Learning Outcomes in a University Introductory Earth Science Course

    Science.gov (United States)

    Forcino, Frank L.

    2013-01-01

    Laboratory sections of university Earth science courses provide hands-on, inquiry-based activities for students in support of lecture and discussion. Here, I compare student conceptual knowledge outcomes of laboratory sections by administering an independent concept inventory at the beginning and end of two courses: one that had a lecture and a…

  16. Developing and Supporting Students' Autonomy to Plan, Perform, and Interpret Inquiry-Based Biochemistry Experiments

    Science.gov (United States)

    Silva, Thanuci; Galembeck, Eduardo

    2017-01-01

    Laboratory sessions are designed to develop the experimental skills and the acquaintance with instruments that may contribute to a successful career in Biochemistry and associated fields. This study is a report on improving a traditional Biochemistry course by devising the laboratory sessions as an inquiry-based environment to develop the…

  17. 临床生物化学室间质评回报结果分析%External Quality Assessment of Clinical Biochemistry Laboratories

    Institute of Scientific and Technical Information of China (English)

    魏琦; 常中宝; 黎春宝; 张明亮; 王春亮; 袁征; 申梦来

    2015-01-01

    Objective:To analyze the results of clinical laboratorycs external quality assessment by using the Industry Standard WS/T403-2012 and six sigma quality management criterion.Methods:Data were collected from routine internal quality control and external quality assess-ment in clinical chemistry laboratory in 2014.Allowable analytical error was defined according to the Industry Standard WS/T403-2012.Inde-xes such asσlevel, quality goal index(QGI),priority improvement measure and performance evaluation were calculated in projects of external quality assessment launched in this department.Results:In 22 routine tests,8 analytes were reached more than 6 Sigma Quality Level,5 analytes were reached more than 3 Sigma Quality Level ,the other 9 analytes were less than 3 Sigma metrics.Conclusion:Application of industry standard WS/T403-2012 and 6 Sigma methodology can improve the level of quality management in clinical chemistry laboratory.%目的:根据行业标准WS/T403-2012和6σ标准分析临床生物化学室间质评回报数据。方法:收集2014年度临床生化检验项目室内质量控制及室间质量评价数据;按照行业标准WS/T403-2012中允许总误差( TEa )标准,计算该室已开展的室间质评项目进行σ水平、质量目标指数( QGI)、优先改进措施及性能评定等指标。结果:全部22个常规生化检测项目,其中8个项目的σ水平>6σ,5个项目的σ水平>3σ,其余9个项目均<3σ。结论:应用行业标准WS/T403-2012和6σ标准分析室间质评数据,有助于了解和提高临床生化检验质量水平。

  18. Limits on WIMP cross-sections from the NAIAD experiment at the Boulby Underground Laboratory

    CERN Document Server

    Alner, G J; Arnison, G J; Barton, J C; Bewick, A; Bungau, C; Camanzi, B; Carson, M J; Davidge, D; Daw, E; Dawson, J V; Davies, G J; Davies, J C; Duffy, C; Durkin, T J; Gamble, T; Hart, S P; Hollingworth, R; Homer, G J; Howard, A S; Ivaniouchenkov, Yu; Jones, W G; Joshi, M K; Kirkpatrick, J; Kudryavtsev, V A; Lawson, T B; Lebedenko, V; Lehner, M J; Lewin, J D; Lightfoot, P K; Liubarsky, I; Lüscher, R; McMillan, J E; Morgan, B; Murphy, A; Nickolls, A; Nicklin, G; Paling, S M; Preece, R M; Quenby, J J; Roberts, J W; Robinson, M; Smith, N J T; Smith, P F; Spooner, N J C; Sumner, T J; Tovey, Daniel R; Tziaferi, E

    2005-01-01

    The NAIAD experiment (NaI Advanced Detector) for WIMP dark matter searches at the Boulby Underground Laboratory (North Yorkshire, UK) ran from 2000 until 2003. A total of 44.9 kg x years of data collected with 2 encapsulated and 4 unencapsulated NaI(Tl) crystals with high light yield were included in the analysis. We present final results of this analysis carried out using pulse shape discrimination. No signal associated with nuclear recoils from WIMP interactions was observed in any run with any crystal. This allowed us to set upper limits on the WIMP-nucleon spin-independent and WIMP-proton spin-dependent cross-sections. The NAIAD experiment has so far imposed the most stringent constraints on the spin-dependent WIMP-proton cross-section.

  19. Thiol biochemistry of prokaryotes

    Science.gov (United States)

    Fahey, Robert C.

    1986-01-01

    The present studies have shown that GSH metabolism arose in the purple bacteria and cyanobacteria where it functions to protect against oxygen toxicity. Evidence was obtained indicating that GSH metabolism was incorporated into eucaryotes via the endosymbiosis giving rise to mitochrondria and chloroplasts. Aerobic bacteria lacking GSH utilize other thiols for apparently similar functions, the thiol being coenzyme A in Gram positive bacteria and chi-glutamylcysteine in the halobacteria. The thiol biochemistry of prokaryotes is thus seen to be much more highly diversified than that of eucaryotes and much remains to be learned about this subject.

  20. Towards quantitative biochemistry: research and reports in biochemistry

    Directory of Open Access Journals (Sweden)

    Dokholyan

    2011-05-01

    Full Text Available Nikolay V DokholyanDepartment of Biochemistry and Biophysics, University of North Carolina, School of Medicine, Chapel Hill, NC, USASince the mid twentieth century the field of biochemistry has evolved significantly (Figure 1. Advances made in the fields of structural, chemical, and systems biology, physics, and computation have shaped the new field of biochemistry such that the emphasis became the cellular-wide view of molecular life at the atomic scale. The principal paradigm in the field shifted from focusing on a single or a few biological molecules to focusing on their cellular- and intracellular-wide networks. This shift has resulted in more interdisciplinary approaches to biochemical research, which nowadays combines techniques and strategies from a broad range of fields. The evolution of the field of biochemistry resulted in closer connections to other fields, such as medicinal chemistry, physiology, and medicine. Biochemistry is also one of the central components of the emerging translational medicine paradigm.

  1. The Biochemistry of Mitosis

    Science.gov (United States)

    Wieser, Samuel; Pines, Jonathon

    2015-01-01

    In this article, we will discuss the biochemistry of mitosis in eukaryotic cells. We will focus on conserved principles that, importantly, are adapted to the biology of the organism. It is vital to bear in mind that the structural requirements for division in a rapidly dividing syncytial Drosophila embryo, for example, are markedly different from those in a unicellular yeast cell. Nevertheless, division in both systems is driven by conserved modules of antagonistic protein kinases and phosphatases, underpinned by ubiquitin-mediated proteolysis, which create molecular switches to drive each stage of division forward. These conserved control modules combine with the self-organizing properties of the subcellular architecture to meet the specific needs of the cell. Our discussion will draw on discoveries in several model systems that have been important in the long history of research on mitosis, and we will try to point out those principles that appear to apply to all cells, compared with those in which the biochemistry has been specifically adapted in a particular organism. PMID:25663668

  2. Laboratory of Cell and Molecular Biology

    Data.gov (United States)

    Federal Laboratory Consortium — The Laboratory of Cell and Molecular Biology investigates the organization, compartmentalization, and biochemistry of eukaryotic cells and the pathology associated...

  3. Lignin biochemistry and soil N determine crop residue decomposition and soil priming

    Science.gov (United States)

    Cropping history can affect soil properties, including available N, but little is known about the interactive effects of residue biochemistry, temperature and cropping history on residue decomposition. A laboratory incubation examined the role of residue biochemistry and temperature on the decomposi...

  4. A biochemistry discipline designed for the nutrition course

    Directory of Open Access Journals (Sweden)

    A.A.G. Bianco

    2004-05-01

    Full Text Available Biochemistry is widely considered an essential background in a Nutrition Course framework. At theFaculdade de Saude Publica, USP, it is a direct requirement to eight disciplines of the syllabus and anindirect requirement to another nine disciplines. Nevertheless, a previous interview study involvingNutrition students and Nutritionists revealed a contradictory image of Biochemistry. Although stu-dents and Nutritionists admitted the important role played by Biochemistry, most of the respondentsdeclared that they could not foresee any application of Biochemical contents in their professional life.Aiming to change this situation, a deep intervention in the Biochemistry discipline was carried on.The discipline was planned in such a way that all the contents to be taught was directly derived fromsubjects or situations matching the interests of nutrition students. Instead of a classical lecture basedcourse, collaborative learning was the methodological choice, taking advantage of practical activitiesinvolving educational software and laboratory work as well. The course was carried on in 180 hoursand a variety of strategies were employed, especially small group discussion and problem solving. Thestudents were given a booklet containing all the exercises and problems, which acted as course guide.At the end of the course, an evaluation survey was carried out. It is noticeable that, according tostudents answers: 100% agreed that Biochemistry was intimately linked to Nutrition; 83% appreciatedthe didactical methodologies employed; 89% would like to continue studying Biochemistry in a furtherdiscipline; 96% declared that the discipline has raised their interest in Biochemistry. In respect tothe assessment of the students, these results are in accordance with the opinion of teachers and TAsengaged in restructuring Biochemistry courses.

  5. [Research activity in clinical biochemistry

    DEFF Research Database (Denmark)

    Jorgensen, H.L.; Larsen, B.; Ingwersen, P.

    2008-01-01

    BACKGROUND: Quantitative bibliometric measurements of research activity are frequently used, e.g. for evaluating applicants for academic positions. The purpose of this investigation is to assess research activity within the medical speciality of Clinical Biochemistry by comparing it with a matched...... Clinical Biochemistry, 57 fulfilled the inclusion criteria. Each of these 57 was matched according to medical title with two randomly chosen specialists from other specialities, totaling 114. Using Medline and the Web of Science, the number of publications and the number of citations were then ascertained....... RESULTS: 25% of the 11,691 specialists held a PhD degree or doctoral degree, DMSci, (Clinical Biochemistry: 61%). The 171 specialists included in the study had 9,823 papers in Medline and 10,140 papers in the Web of Science. The number of Medline papers per specialist was 71 for Clinical Biochemistry...

  6. Rastreabilidade metrológica em medicina laboratorial. Estudo de kits de bioquímica utilizados em laboratórios de análises clínicas | Metrological traceability in laboratory medicine: study of biochemistry kits used in clinical pathology laboratories

    Directory of Open Access Journals (Sweden)

    Maria Cristina Ferreira Pessoa

    2016-11-01

    Full Text Available A importância da rastreabilidade metrológica na produção de kits (conjuntos diagnósticos não é de domínio da comunidade laboratorial e, consequentemente, sua influência em validações de métodos e produção de resultados exatos e comparáveis nos laboratórios é pouco discutida. Este estudo espera contribuir para a disseminação deste conhecimento e evidenciar, para a comunidade de laboratoristas, a importância de realizar seus exames com conjuntos diagnósticos que utilizem Material de Referência Certificado (MRC ou Método de Referência (MetR em sua produção. O presente estudo evidencia que a rastreabilidade metrológica foi exclusivamente demonstrada pelo uso de MRC em 57% dos casos e em 26% pelo uso de MetR. Para os conjuntos diagnósticos em que os MRC foram utilizados no processo de fabricação, estes tiveram origem exclusiva do NIST (National Institute os Standards and Technology. Os resultados do estudo indicam que 46% dos conjuntos diagnósticos reportados no ensaio de proficiência da ControlLab – Bioquímica I são produzidos no Brasil, o que demonstra a importância desta indústria no nosso mercado. Entretanto, no que concerne à informação sobre rastreabilidade metrológica, dos 121 conjuntos diagnósticos pesquisados, 14% não informam rastreabilidade e todos são produtos nacionais, sugerindo que existem fabricantes que não estão atentos à disseminação desta informação como forma de atestar a qualidade do seu produto. ============================================= The importance of metrological traceability in the manufacture of diagnostic kits is not well understood in the laboratory community. Therefore, its influence on method validation and the production of accurate and comparable results in clinical laboratories is rarely discussed. This study aims to contribute to the dissemination of the knowledge of metrological traceability and emphasize the importance of using Certified Reference Materials

  7. Biochemistry of Statins.

    Science.gov (United States)

    Egom, Emmanuel Eroume A; Hafeez, Hafsa

    2016-01-01

    Cardiovascular disease (CVD) is the leading cause of morbidity and mortality worldwide. Elevated blood lipids may be a major risk factor for CVD. Due to consistent and robust association of higher low-density lipoprotein (LDL)-cholesterol levels with CVD across experimental and epidemiologic studies, therapeutic strategies to decrease risk have focused on LDL-cholesterol reduction as the primary goal. Current medication options for lipid-lowering therapy include statins, bile acid sequestrants, a cholesterol-absorption inhibitor, fibrates, nicotinic acid, and omega-3 fatty acids, which all have various mechanisms of action and pharmacokinetic properties. The most widely prescribed lipid-lowering agents are the HMG-CoA reductase inhibitors, or statins. Since their introduction in the 1980s, statins have emerged as the one of the best-selling medication classes to date, with numerous trials demonstrating powerful efficacy in preventing cardiovascular outcomes (Kapur and Musunuru, 2008 [1]). The statins are commonly used in the treatment of hypercholesterolemia and mixed hyperlipidemia. This chapter focuses on the biochemistry of statins including their structures, pharmacokinetics, and mechanism of actions as well as the potential adverse reactions linked to their clinical uses.

  8. Seismic imaging of sandbox experiments - laboratory hardware setup and first reflection seismic sections

    Science.gov (United States)

    Krawczyk, C. M.; Buddensiek, M.-L.; Oncken, O.; Kukowski, N.

    2012-10-01

    With the study and technical development introduced here, we combine analogue sandbox simulation techniques with seismic physical modelling of sandbox models. For that purpose, we designed and developed a new mini-seismic facility for laboratory use, comprising a seismic tank, a PC-driven control unit, a positioning system, and piezo-electric transducers used here the first time in an array mode. To assess the possibilities and limits of seismic imaging of small-scale structures in sandbox models, different geometry setups were tested in the first experiments that also tested the proper functioning of the device and studied the seismo-elastic properties of the granular media used. Simple two-layer models of different materials and layer thicknesses as well as a more complex model comprising channels and shear zones were tested using different acquisition geometries and signal properties. We suggest using well sorted and well rounded grains with little surface roughness (glass beads). Source receiver-offsets less than 14 cm for imaging structures as small as 2.0-1.5 mm size have proven feasible. This is the best compromise between wide beam and high energy output, and being applicable with a consistent waveform. Resolution of the interfaces of layers of granular materials depends on the interface preparation rather than on the material itself. Flat grading of interfaces and powder coverage yields the clearest interface reflections. Finally, sandbox seismic sections provide images of very good quality showing constant thickness layers as well as predefined channel structures and fault traces from shear zones. Since these can be regarded in sandbox models as zones of decompaction, they behave as reflectors and can be imaged. The multiple-offset surveying introduced here improves the quality with respect to S/N-ratio and source signature even more; the maximum depth penetration in glass bead layers thereby amounts to 5 cm. Thus, the presented mini-seismic device is

  9. Seismic imaging of sandbox experiments – laboratory hardware setup and first reflection seismic sections

    Directory of Open Access Journals (Sweden)

    N. Kukowski

    2012-10-01

    Full Text Available With the study and technical development introduced here, we combine analogue sandbox simulation techniques with seismic physical modelling of sandbox models. For that purpose, we designed and developed a new mini-seismic facility for laboratory use, comprising a seismic tank, a PC-driven control unit, a positioning system, and piezo-electric transducers used here the first time in an array mode. To assess the possibilities and limits of seismic imaging of small-scale structures in sandbox models, different geometry setups were tested in the first experiments that also tested the proper functioning of the device and studied the seismo-elastic properties of the granular media used. Simple two-layer models of different materials and layer thicknesses as well as a more complex model comprising channels and shear zones were tested using different acquisition geometries and signal properties. We suggest using well sorted and well rounded grains with little surface roughness (glass beads. Source receiver-offsets less than 14 cm for imaging structures as small as 2.0–1.5 mm size have proven feasible. This is the best compromise between wide beam and high energy output, and being applicable with a consistent waveform. Resolution of the interfaces of layers of granular materials depends on the interface preparation rather than on the material itself. Flat grading of interfaces and powder coverage yields the clearest interface reflections. Finally, sandbox seismic sections provide images of very good quality showing constant thickness layers as well as predefined channel structures and fault traces from shear zones. Since these can be regarded in sandbox models as zones of decompaction, they behave as reflectors and can be imaged. The multiple-offset surveying introduced here improves the quality with respect to S/N-ratio and source signature even more; the maximum depth penetration in glass bead layers thereby amounts to 5 cm. Thus, the presented mini

  10. Seismic imaging of sandbox experiments - laboratory hardware setup and first reflection seismic sections

    Science.gov (United States)

    Krawczyk, C. M.; Buddensiek, M.-L.; Oncken, O.; Kukowski, N.

    2013-02-01

    With the study and technical development introduced here, we combine analogue sandbox simulation techniques with seismic physical modelling of sandbox models. For that purpose, we designed and developed a new mini-seismic facility for laboratory use, comprising a seismic tank, a PC-driven control unit, a positioning system, and piezoelectric transducers used here for the first time in an array mode. To assess the possibilities and limits of seismic imaging of small-scale structures in sandbox models, different geometry setups were tested in the first 2-D experiments that also tested the proper functioning of the device and studied the seismo-elastic properties of the granular media used. Simple two-layer models of different materials and layer thicknesses as well as a more complex model comprising channels and shear zones were tested using different acquisition geometries and signal properties. We suggest using well sorted and well rounded grains with little surface roughness (glass beads). Source receiver-offsets less than 14 cm for imaging structures as small as 2.0-1.5 mm size have proven feasible. This is the best compromise between wide beam and high energy output, and is applicable with a consistent waveform. Resolution of the interfaces of layers of granular materials depends on the interface preparation rather than on the material itself. Flat grading of interfaces and powder coverage yields the clearest interface reflections. Finally, sandbox seismic sections provide images of high quality showing constant thickness layers as well as predefined channel structures and indications of the fault traces from shear zones. Since these were artificially introduced in our test models, they can be regarded as zones of disturbance rather than tectonic shear zones characterized by decompaction. The multiple-offset surveying introduced here, improves the quality with respect to S / N ratio and source signature even more; the maximum depth penetration in glass

  11. Seismic imaging of sandbox experiments – laboratory hardware setup and first reflection seismic sections

    Directory of Open Access Journals (Sweden)

    C. M. Krawczyk

    2013-02-01

    Full Text Available With the study and technical development introduced here, we combine analogue sandbox simulation techniques with seismic physical modelling of sandbox models. For that purpose, we designed and developed a new mini-seismic facility for laboratory use, comprising a seismic tank, a PC-driven control unit, a positioning system, and piezoelectric transducers used here for the first time in an array mode. To assess the possibilities and limits of seismic imaging of small-scale structures in sandbox models, different geometry setups were tested in the first 2-D experiments that also tested the proper functioning of the device and studied the seismo-elastic properties of the granular media used. Simple two-layer models of different materials and layer thicknesses as well as a more complex model comprising channels and shear zones were tested using different acquisition geometries and signal properties. We suggest using well sorted and well rounded grains with little surface roughness (glass beads. Source receiver-offsets less than 14 cm for imaging structures as small as 2.0–1.5 mm size have proven feasible. This is the best compromise between wide beam and high energy output, and is applicable with a consistent waveform. Resolution of the interfaces of layers of granular materials depends on the interface preparation rather than on the material itself. Flat grading of interfaces and powder coverage yields the clearest interface reflections. Finally, sandbox seismic sections provide images of high quality showing constant thickness layers as well as predefined channel structures and indications of the fault traces from shear zones. Since these were artificially introduced in our test models, they can be regarded as zones of disturbance rather than tectonic shear zones characterized by decompaction. The multiple-offset surveying introduced here, improves the quality with respect to S / N ratio and source signature even more; the maximum depth

  12. THE PRESENCE OF BIOCHEMISTRY EXPERIMENTS IN TEXTBOOKS

    Directory of Open Access Journals (Sweden)

    C. E.S. Rocha

    2015-08-01

    Full Text Available INTRODUCTION: Being present in high school in chemistry and biology disciplines, biochemistry is to explain the chemical and biological reactions in cells. His concepts are seen as abstract and difficult to understand and can be contextualized through experimentation. Experimentation is used in the classroom to allow students to view the applicability of the contents through observation, creating hypotheses, discussion and conclusions. OBJECTIVES: The aim of this study was to evaluate the presence of experimentation in biochemistry content  in high school books. MATERIALS AND METHODS: In this way, it was analyzed the presence of experimental activities in the textbooks approved by the National Textbook Plan 2015 "Biology 1", authored by César da Silva Junior, Sezar Sasson and Nelson Caldini Junior, and in the book " Chemistry - Volume 3 "by Martha Reis. RESULTS: At the end of the unit where the biochemical concepts are covered in the book "Biology 1" is presented an interdisciplinary project called "Enzymes - chemical tools of life" that proposes four experiments on catalase, pH and contact surface enzyme-substrate. They are easy activities to be implemented with affordable materials. Already at the Chemistry book, when addressing the biochemistry there is only one proposal for experimental activity called "Glycolic Extract of milk proteins". In this case, we need materials like food processor and enameled pots, which can hinder your performance in virtual classes, however, the experiment is easy to perform. Both books contain questions after the experimental proposals enhancing the questioning of the activities and understanding the contents addressed. CONCLUSIONS: The present experimental activities in the textbooks are mostly easy to apply and is not required sophisticated equipment of Sciences laboratories. It also allows the contextualization of theory and practice enhancing the process of teaching and learning with the proposals made.

  13. Does breast feeding influence liver biochemistry?

    DEFF Research Database (Denmark)

    Jørgensen, Marianne Hørby; Ott, Peter; Juul, Anders

    2003-01-01

    It is assumed that early feeding can affect liver biochemistry because breast-fed infants have a higher risk of hyperbilirubinemia than formula-fed infants. The authors sought to determine how feeding mode affected liver biochemistry in healthy term infants.......It is assumed that early feeding can affect liver biochemistry because breast-fed infants have a higher risk of hyperbilirubinemia than formula-fed infants. The authors sought to determine how feeding mode affected liver biochemistry in healthy term infants....

  14. Soil Microbiology, Ecology, and Biochemistry

    Science.gov (United States)

    The 4th edition of Soil Microbiology, Ecology, and Biochemistry Edited by Eldor Paul continues in the vein of the 3rd edition by providing an excellent, broad-reaching introduction to soil biology. The new edition improves on the previous by providing extensive supplementary materials, links to outs...

  15. Commentary: Biochemistry Re-Natured

    Science.gov (United States)

    White, Harold B.

    2010-01-01

    In his last commentary on "Biochemistry Denatured," this author dealt with his perception that college students today have spent too little of their childhood years playing outside in nature and as a consequence have not learned basic things about the world from personal experience. This "nature-deficit disorder" removes many opportunities for…

  16. Promising New Directions in Biochemistry

    Science.gov (United States)

    Olivera, Baldomero M.

    2003-01-01

    "Biochemistry," by Lubert Stryer, has become one of the standard textbooks for the field. The Fifth Edition has two new authors: Jeremy Berg, Professor and Director of Biophysics and Biophysical Chemistry at Johns Hopkins University School of Medicine; and John Tymoczko, the Towsley Professor of Biology at Carleton College. The new edition does,…

  17. Laboratory analysis of dental sections made with commercial tungsten carbide burs coated with HFCVD diamond

    Energy Technology Data Exchange (ETDEWEB)

    Maass, F [Departamento de Fisica, Universidad de Antofagasta, Av. Angamos 601, Antofagasta (Chile); Aguilera, Y [Departamento de Ingenieria Industrial, Universidad de Antofagasta, Av. Angamos 601, Antofagasta (Chile); Avaria, J [Departamento de OdontologIa, Universidad de Antofagasta, Av. Angamos 601, Antofagasta (Chile)], E-mail: fdmaass@uantof.cl

    2008-11-01

    The objective of this study was to determine the cutting power of diamond burs obtained by the HFCVD deposition process. Diamond was deposited on the active part of each of a series of 10 commonly used Tungsten Carbide (WC) commercial burs. The quality of the section was compared with sections made by commonly used commercial burs, employing fresh human molars and a standard device [1]. Both burs and sections were analysed by using SEM and EDX techniques. The quality and tension of the deposited diamond coatings were analyzed by Raman Spectroscopy. The optimal thickness of the diamond coating which provided the best durability and finish of the sections was determined by comparative observations of results.

  18. A Two-Week Guided Inquiry Protein Separation and Detection Experiment for Undergraduate Biochemistry

    Science.gov (United States)

    Carolan, James P.; Nolta, Kathleen V.

    2016-01-01

    A laboratory experiment for teaching protein separation and detection in an undergraduate biochemistry laboratory course is described. This experiment, performed in two, 4 h laboratory periods, incorporates guided inquiry principles to introduce students to the concepts behind and difficulties of protein purification. After using size-exclusion…

  19. Seismic imaging of sandbox experiments – laboratory hardware setup and first reflection seismic sections

    OpenAIRE

    Kukowski, N.; Oncken, O.; M.-L. Buddensiek; C. M. Krawczyk

    2012-01-01

    With the study and technical development introduced here, we combine analogue sandbox simulation techniques with seismic physical modelling of sandbox models. For that purpose, we designed and developed a new mini-seismic facility for laboratory use, comprising a seismic tank, a PC-driven control unit, a positioning system, and piezo-electric transducers used here the first time in an array mode. To assess the possibilities and limits of seismic imaging of small-scale structures in sandbox mo...

  20. Seismic imaging of sandbox experiments – laboratory hardware setup and first reflection seismic sections

    OpenAIRE

    C. M. Krawczyk; Buddensiek, M.-L.; Oncken, O.; Kukowski, N.

    2013-01-01

    With the study and technical development introduced here, we combine analogue sandbox simulation techniques with seismic physical modelling of sandbox models. For that purpose, we designed and developed a new mini-seismic facility for laboratory use, comprising a seismic tank, a PC-driven control unit, a positioning system, and piezoelectric transducers used here for the first time in an array mode. To assess the possibilities and limits of seismic imaging of small-scale str...

  1. Chromatin Isolation and DNA Sequence Analysis in Large Undergraduate Laboratory Sections

    Science.gov (United States)

    Hagerman, Ann E.

    1999-10-01

    A pair of exercises that introduce undergraduate students to basic techniques and concepts of molecular biology and that are appropriate for classes with large enrollments are described. One exercise is a simple laboratory experiment in which chromatin is isolated from chicken liver and is resolved into histone proteins and DNA by ion-exchange chromatography. The other is a series of computer simulations that introduce DNA sequencing, mapping, and sequence analysis to the students. The final step of the simulation is submission of a sequence to a database on the World Wide Web for identification of the protein product of the gene.

  2. Measurement of K Shell Photoelectric Cross Sections at a K Edge--A Laboratory Experiment

    Science.gov (United States)

    Nayak, S. V.; Badiger, N. M.

    2007-01-01

    We describe in this paper a new method for measuring the K shell photoelectric cross sections of high-Z elemental targets at a K absorption edge. In this method the external bremsstrahlung (EB) photons produced in the Ni target foil by beta particles from a weak[superscript 90]Sr-[superscript 90]Y beta source are passed through an elemental target…

  3. Wanderings in biochemistry.

    Science.gov (United States)

    Lengyel, Peter

    2014-07-11

    My Ph.D. thesis in the laboratory of Severo Ochoa at New York University School of Medicine in 1962 included the determination of the nucleotide compositions of codons specifying amino acids. The experiments were based on the use of random copolyribonucleotides (synthesized by polynucleotide phosphorylase) as messenger RNA in a cell-free protein-synthesizing system. At Yale University, where I joined the faculty, my co-workers and I first studied the mechanisms of protein synthesis. Thereafter, we explored the interferons (IFNs), which were discovered as antiviral defense agents but were revealed to be components of a highly complex multifunctional system. We isolated pure IFNs and characterized IFN-activated genes, the proteins they encode, and their functions. We concentrated on a cluster of IFN-activated genes, the p200 cluster, which arose by repeated gene duplications and which encodes a large family of highly multifunctional proteins. For example, the murine protein p204 can be activated in numerous tissues by distinct transcription factors. It modulates cell proliferation and the differentiation of a variety of tissues by binding to many proteins. p204 also inhibits the activities of wild-type Ras proteins and Ras oncoproteins.

  4. Metabolism and biochemistry in hypogravity

    Science.gov (United States)

    Leach, Carolyn S.

    The headward shift of body fluid and increase in stress-related hormones that occur in hypogravity bring about a number of changes in metabolism and biochemistry of the human body. Such alterations may have important effects on health during flight and during a recovery period after return to Earth. Body fluid and electrolytes are lost, and blood levels of several hormones that control metabolism are altered during space flight. Increased serum calcium may lead to an increased risk of renal stone formation during flight, and altered drug metabolism could influence the efficacy of therapeutic agents. Orthostatic intolerance and an increased risk of fracturing weakened bones are concerns at landing. It is important to understand biochemistry and metabolism in hypogravity so that clinically important developments can be anticipated and prevented or ameliorated.

  5. Metabolism and biochemistry in hypogravity

    Science.gov (United States)

    Leach, Carolyn S.

    1991-01-01

    The headward shift of body fluid and increase in stress-related hormones that occur in hypogravity bring about a number of changes in metabolism and biochemistry of the human body. Such alterations may have important effects on health during flight and during a recovery period after return to earth. Body fluid and electrolytes are lost, and blood levels of several hormones that control metabolism are altered during space flight. Increased serum calcium may lead to an increased risk of renal stone formation during flight, and altered drug metabolism could influence the efficacy of therapeutic agents. Orthostatic intolerance and an increased risk of fracturing weakened bones are concerns at landing. It is important to understand biochemistry and metabolism in hypogravity so that clinically important developments can be anticipated and prevented or ameliorated.

  6. Gender Performance Differences in Biochemistry

    Science.gov (United States)

    Rauschenberger, Matthew M.; Sweeder, Ryan D.

    2010-01-01

    This study examined the historical performance of students at Michigan State University in a two-part biochemistry series Biochem I (n = 5,900) and Biochem II (n = 5,214) for students enrolled from 1997 to 2009. Multiple linear regressions predicted 54.9-87.5% of the variance in student from Biochem I grade and 53.8-76.1% of the variance in…

  7. Instrumentation of Microscale Techniques for Biochemistry Teaching at FES Zaragoza, UNAM

    Directory of Open Access Journals (Sweden)

    Araceli García-del Valle

    2015-02-01

    Full Text Available Biochemistry teaching requires many laboratory sessions where theoretical knowledge may be put on test. At the same time, there is always some risk due to exposure to toxic materials, dangerous chemicals storage and waste disposal. Compliance with new regulations to prevent environmental contamination may also constitute a real hindrance for biochemistry teaching as experimental science. Therefore, we have designed microscale techniques, in order to reduce costs as well as the negative impact of laboratory practical sessions due to risk and environmental contamination. To develop microscale techniques does not only mean to reduce equipment size and amount of the reagents that are required for the usual experiments. Microscale techniques serve particularly well as a motivating approach to experimental biochemistry teaching that produces highly motivated students at the same time that requires minor costs, decreases working time, laboratory space, reagents volume and diminishes the generation of dangerous waste. We have demonstrated all these positive effects in biochemistry teaching and prompted the formal implementation of microscale techniques into the formal activities from the Cell and Tissue Biochemistry Laboratory I (BCT-I from the Chemistry, Pharmacy and Biology (QFB curricula at the National Autonomous University of Mexico (UNAM. First, we reviewed the BCT-I manual, choosing all the laboratory practices that might be microscaled. Then, we elaborated and validated all necessary protocols to analyse linearity, accuracy and reproducibility of the determinations, demonstrating that microscale techniques allow truthful results, comparable to full scale techniques.

  8. THE CYBERSPACE IN THE CONTINUED CLINICAL BIOCHEMISTRY EDUCATION

    Directory of Open Access Journals (Sweden)

    J.M. Martins

    2008-05-01

    Full Text Available The cybernetic spaces simulate the real world with interactive multimedia. This work  has been applied since January, 2007 on the curricular student’s apprenticeship at high school and graduation, in the site “bioq.educacao.biz/ULAB-HC-UFPE”. It has been developed to provide continuity to the technical-scientific learning of students and professionals, and also to improve their human social relations on the  labour  environment.  It’s comprises a virtual space, destined to communication and collective building of knowledge on the clinical biochemistry.   It’s about an interactive environment which allows the users registered as coordinator professor (professional  or the scientist student (trainee,  unlimited access to  posting contents (classes, texts, presentations, animations, consultations, non-synchronic discussions (on orkut, forums, e-mail and synchronic discussions (on chats, videoconferences. After a few live tutorials  about new  input in this environment, and the use of the new learning tool,  the collective building of knowledge on cyberspace begins. As a trainee’s program task, the scientist student would have to build a space of his own, under guidance and supervision of the coordinator teachers.  The cyberspace efficiency was evaluated from reports collected in February, 2008: the adherence to this  work was satisfactory, regarding this period, with 68 registered users, 870 accesses and 52 contents available on the several sections of the virtual laboratory. Our work is still being applied, and new adhesions are  happening everyday. We intend to amplify this cyber environment in order to make it a  permanent  continued education site on the health area.  From interest contracts and common knowledge,  the technological interfaces constitute an interaction, in which everyone is a potential author.  Keywords: Cyberspace, online biochemistry education, continued education.

  9. The Consequence of Combined Pain and Stress on Work Ability in Female Laboratory Technicians: A Cross-Sectional Study.

    Science.gov (United States)

    Jay, Kenneth; Friborg, Maria Kristine; Sjøgaard, Gisela; Jakobsen, Markus Due; Sundstrup, Emil; Brandt, Mikkel; Andersen, Lars Louis

    2015-12-11

    Musculoskeletal pain and stress-related disorders are leading causes of impaired work ability, sickness absences and disability pensions. However, knowledge about the combined detrimental effect of pain and stress on work ability is lacking. This study investigates the association between pain in the neck-shoulders, perceived stress, and work ability. In a cross-sectional survey at a large pharmaceutical company in Denmark 473 female laboratory technicians replied to questions about stress (Perceived Stress Scale), musculoskeletal pain intensity (scale 0-10) of the neck and shoulders, and work ability (Work Ability Index). General linear models tested the association between variables. In the multi-adjusted model, stress (p work ability index score, and there was no significant stress by pain interaction (p = 0.32). Work ability decreased gradually with both increased stress and pain. Workers with low stress and low pain had the highest Work Ability Index score (44.6 (95% CI 43.9-45.3)) and workers with high stress and high pain had the lowest score (32.7 (95% CI 30.6-34.9)). This cross-sectional study indicates that increased stress and musculoskeletal pain are independently associated with lower work ability in female laboratory technicians.

  10. The Consequence of Combined Pain and Stress on Work Ability in Female Laboratory Technicians: A Cross-Sectional Study

    Directory of Open Access Journals (Sweden)

    Kenneth Jay

    2015-12-01

    Full Text Available Musculoskeletal pain and stress-related disorders are leading causes of impaired work ability, sickness absences and disability pensions. However, knowledge about the combined detrimental effect of pain and stress on work ability is lacking. This study investigates the association between pain in the neck-shoulders, perceived stress, and work ability. In a cross-sectional survey at a large pharmaceutical company in Denmark 473 female laboratory technicians replied to questions about stress (Perceived Stress Scale, musculoskeletal pain intensity (scale 0–10 of the neck and shoulders, and work ability (Work Ability Index. General linear models tested the association between variables. In the multi-adjusted model, stress (p < 0.001 and pain (p < 0.001 had independent main effects on the work ability index score, and there was no significant stress by pain interaction (p = 0.32. Work ability decreased gradually with both increased stress and pain. Workers with low stress and low pain had the highest Work Ability Index score (44.6 (95% CI 43.9–45.3 and workers with high stress and high pain had the lowest score (32.7 (95% CI 30.6–34.9. This cross-sectional study indicates that increased stress and musculoskeletal pain are independently associated with lower work ability in female laboratory technicians.

  11. Laboratory Measurements And Modeling Of Molecular Photoabsorption Cross Sections In The Ultraviolet: N2, So2, S2, Co2

    Science.gov (United States)

    Stark, Glenn; Smith, P. L.; Lewis, B. R.; Heays, A. N.; Blackie, D.; Pickering, J.

    2010-10-01

    Our research program comprises the measurement and modeling of ultraviolet molecular photoabsorption cross sections with the highest practical resolution. Measurement and modeling efforts on molecular nitrogen, sulfur dioxide, diatomic sulfur, and carbon dioxide are in progress. N2: We measure line f-values and widths within the complex spectrum between 80 and 100 nm. Our measurements are incorporated into a theoretical model of the absorption spectrum of N2 which has established the mechanisms responsible for predissociation and reproduces the observed features in all N2 isotopomers as a function of temperature. SO2: We provide astronomers with high-resolution cross section data for the complex ultraviolet SO2 absorption spectrum. Using the Imperial College VUV Fourier transform spectrometer, we have completed and published cross sections from 198 to 325 nm (295 K) and from 199 to 220 nm (160 K). We have completed work on further low-temperature measurements from 220 to 325 nm. S2: Interpretations of atmospheric (Io, Jupiter) S2 absorption features are hindered by a complete lack of laboratory cross sections in the ultraviolet. We have begun to quantify the photoabsorption spectrum of S2 from 200 to 300 nm. We have designed an experimental apparatus to produce a stable column of S2 vapor. Measurements of S2 absorption features at high resolution will be complemented by coupled-channel calculations of the relevant transitions. CO2: The photodissociation of CO2 is a fundamental photochemical process in the atmospheres of Mars and Venus. Our research centers on the measurement of high resolution cross sections from 87 to 120 nm. We have completed measurements at 295 K and 195 K over the 106 to 120 nm region. We have recently completed preliminary room temperature measurements in the 87 to 106 nm region. We acknowledge grant support from NASA (NNX08AE78G), STFC-PPARC, and ARC (DP0558962).

  12. Pulsed and monoenergetic beams for neutron cross-section measurements using activation and scattering techniques at Triangle Universities Nuclear Laboratory

    Science.gov (United States)

    Hutcheson, A.; Angell, C. T.; Becker, J. A.; Boswell, M.; Crowell, A. S.; Dashdorj, D.; Fallin, B.; Fotiades, N.; Howell, C. R.; Karwowski, H. J.; Kelley, J. H.; Kiser, M.; Macri, R. A.; Nelson, R. O.; Pedroni, R. S.; Tonchev, A. P.; Tornow, W.; Vieira, D. J.; Weisel, G. J.; Wilhelmy, J. B.

    2007-08-01

    In support of the Stewardship Science Academic Alliances initiative, an experimental program has been developed at Triangle Universities Nuclear Laboratory (TUNL) to measure (n,xn) cross-sections with both in-beam and activation techniques with the goal of improving the partial cross-section database for the NNSA Stockpile Stewardship Program. First experimental efforts include excitation function measurements on 235,238U and 241Am using pulsed and monoenergetic neutron beams with En = 5-15 MeV. Neutron-induced partial cross-sections were measured by detecting prompt γ rays from the residual nuclei using various combinations of clover and planar HPGe detectors in the TUNL shielded neutron source area. Complimentary activation measurements using DC neutron beams have also been performed in open geometry in our second target area. The neutron-induced activities were measured in the TUNL low-background counting area. In this presentation, we include detailed information about the irradiation procedures and facilities and preliminary data on first measurements using this capability.

  13. Pulsed and monoenergetic beams for neutron cross-section measurements using activation and scattering techniques at Triangle Universities Nuclear Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Hutcheson, A. [Triangle Universities Nuclear Laboratory, P.O. Box 90308, Durham, NC 27708 (United States)]. E-mail: hutch@tunl.duke.edu; Angell, C.T. [Triangle Universities Nuclear Laboratory, P.O. Box 90308, Durham, NC 27708 (United States); Becker, J.A. [Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550 (United States); Boswell, M. [Triangle Universities Nuclear Laboratory, P.O. Box 90308, Durham, NC 27708 (United States); Crowell, A.S. [Triangle Universities Nuclear Laboratory, P.O. Box 90308, Durham, NC 27708 (United States); Dashdorj, D. [Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550 (United States); Fallin, B. [Triangle Universities Nuclear Laboratory, P.O. Box 90308, Durham, NC 27708 (United States); Fotiades, N. [Los Alamos National Laboratory, P.O. Box 1663, Los Alamos, NM 87545 (United States); Howell, C.R.; Karwowski, H.J.; Kelley, J.H.; Kiser, M. [Triangle Universities Nuclear Laboratory, P.O. Box 90308, Durham, NC 27708 (United States); Macri, R.A. [Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550 (United States); Nelson, R.O. [Los Alamos National Laboratory, P.O. Box 1663, Los Alamos, NM 87545 (United States); Pedroni, R.S. [NC A and T State University, 1601 East Market Street, Greensboro, NC 27411 (United States); Tonchev, A.P.; Tornow, W. [Triangle Universities Nuclear Laboratory, P.O. Box 90308, Durham, NC 27708 (United States); Vieira, D.J. [Los Alamos National Laboratory, P.O. Box 1663, Los Alamos, NM 87545 (United States); Weisel, G.J. [Penn State Altoona, 3000 Ivyside Park, Altoona, PA 16601 (United States); Wilhelmy, J.B. [Los Alamos National Laboratory, P.O. Box 1663, Los Alamos, NM 87545 (United States)

    2007-08-15

    In support of the Stewardship Science Academic Alliances initiative, an experimental program has been developed at Triangle Universities Nuclear Laboratory (TUNL) to measure (n,xn) cross-sections with both in-beam and activation techniques with the goal of improving the partial cross-section database for the NNSA Stockpile Stewardship Program. First experimental efforts include excitation function measurements on {sup 235,238}U and {sup 241}Am using pulsed and monoenergetic neutron beams with E {sub n} = 5-15 MeV. Neutron-induced partial cross-sections were measured by detecting prompt {gamma} rays from the residual nuclei using various combinations of clover and planar HPGe detectors in the TUNL shielded neutron source area. Complimentary activation measurements using DC neutron beams have also been performed in open geometry in our second target area. The neutron-induced activities were measured in the TUNL low-background counting area. In this presentation, we include detailed information about the irradiation procedures and facilities and preliminary data on first measurements using this capability.

  14. Teaching biochemistry to medical students in Singapore--from organic chemistry to problem-based learning.

    Science.gov (United States)

    Khoo, H E

    2005-07-01

    The medical faculty in the National University of Singapore started in 1905 but the Chair in Biochemistry was only established in 1927. For many years the biochemistry course consisted of the teaching of the organic chemistry of substances of physiological importance, nutrition, metabolism and hormones. In 1961, clinical biochemistry was introduced and in the 1980s, genetics and molecular biology were included. By then, most of the organic chemistry content had been removed as greater emphasis was placed on clinical correlation. Laboratory classes consisted of mock glucose tolerance tests and the measurement of various enzymes. By the 1990s, students were no longer interested in such practical classes, so a bold decision was made around 1995 to remove laboratory classes from the curriculum. Unfortunately, this meant that the medical students who might have been interested in laboratory work could no longer do such work. However, the new curriculum in 1999 gave the department an opportunity to offer a laboratory course as an elective for interested students. This new curriculum adopted an integrated approach with Genetics being taught as part of Paediatrics, and a new module (Structural and Cell Biology) comprising aspects of cell biology and biochemistry was introduced. This module is currently taught by staff from Anatomy, Physiology and Biochemistry. Some biochemistry content is now incorporated into the clinical problem scenarios of problem-based learning such as jaundice, diabetes mellitus, anorexia nervosa, etc. So the evolution of teaching biochemistry to medical students in Singapore has paralleled worldwide trends and moved from the didactic teaching of organic chemistry of biomolecules to problem-based learning using clinical cases.

  15. Utilizing Isolation, Purification, and Characterization of Enzymes as Project-Oriented Labs for Undergraduate Biochemistry

    Science.gov (United States)

    Deal, S. Todd; Hurst, Michael O.

    1997-02-01

    Senior-level biochemistry labs are mostly verification-type laboratories with little chance for exploration. We have developed a project-based biochemistry laboratory which gives them a chance to carry out a major biochemistry project. In the first quarter it is based on the purification of the enzyme lysozyme. The students are given some basic information, and then work out the details of their own procedures, make up their own solutions, and work at their own pace. Students use centrifugation, ion-exchange chromatography, spectral enzyme assays, and SDS-gel electrophoresis to purify and characterize the protein. In the second quarter students are given acid phosphatase and the basic assay for the enzyme, and then develop and carry out a method for determining the kinetic parameters of the enzyme. These experiments continue the development of laboratory independence of the students which steadily progresses in most curriculum

  16. Does breast feeding influence liver biochemistry?

    DEFF Research Database (Denmark)

    Jørgensen, Marianne Hørby; Ott, Peter; Juul, Anders;

    2003-01-01

    It is assumed that early feeding can affect liver biochemistry because breast-fed infants have a higher risk of hyperbilirubinemia than formula-fed infants. The authors sought to determine how feeding mode affected liver biochemistry in healthy term infants....

  17. Teaching Biochemistry Online at Oregon State University

    Science.gov (United States)

    Ahern, Kevin

    2017-01-01

    A strategy for growing online biochemistry courses is presented based on successes in ecampus at Oregon State University. Four free drawing cards were key to the effort--YouTube videos, iTunes U online free course content, an Open Educational Resource textbook--Biochemistry Free and Easy, and a fun set of educational songs known as the Metabolic…

  18. Toward Improving Atmospheric Models and Ozone Projections: Laboratory UV Absorption Cross Sections and Equilibrium Constant of ClOOCl

    Science.gov (United States)

    Wilmouth, D. M.; Klobas, J. E.; Anderson, J. G.

    2015-12-01

    Thirty years have now passed since the discovery of the Antarctic ozone hole, and despite comprehensive international agreements being in place to phase out CFCs and halons, polar ozone losses generally remain severe. The relevant halogen compounds have very long atmospheric lifetimes, which ensures that seasonal polar ozone depletion will likely continue for decades to come. Changes in the climate system can further impact stratospheric ozone abundance through changes in the temperature and water vapor structure of the atmosphere and through the potential initiation of solar radiation management efforts. In many ways, the rate at which climate is changing must now be considered fast relative to the slow removal of halogens from the atmosphere. Photochemical models of Earth's atmosphere play a critical role in understanding and projecting ozone levels, but in order for these models to be accurate, they must be built on a foundation of accurate laboratory data. ClOOCl is the centerpiece of the catalytic cycle that accounts for more than 50% of the chlorine-catalyzed ozone loss in the Arctic and Antarctic stratosphere every spring, and so uncertainties in the ultraviolet cross sections of ClOOCl are particularly important. Additionally, the equilibrium constant of the dimerization reaction of ClO merits further study, as there are important discrepancies between in situ measurements and lab-based models, and the JPL-11 recommended equilibrium constant includes high error bars at atmospherically relevant temperatures (~75% at 200 K). Here we analyze available data for the ClOOCl ultraviolet cross sections and equilibrium constant and present new laboratory spectroscopic results.

  19. Symposium 19: The contributions of the Department of Biochemistry/USP towards Biochemistry teaching

    Directory of Open Access Journals (Sweden)

    Bayardo Baptista Torres

    2014-08-01

    Full Text Available K-Education(Portuguese Chair: V. Trindade Bayardo Torres; Clovis Wannmacher; Denise MacedoThe contributions of the Department of Biochemistry/USP towards Biochemistry teaching.O ensino de Bioquímica nos últimos 20 anosBayardo B. TorresDepartamento de Bioquímica, Instituto de Química, USP. São Paulo, Brazil.Among the contributions of the Department of Biochemistry/USP one must recall:1. Winter school for graduate studentsThis course, now at the ninth edition, is intended for students in the final stage of their Masters or PhD in Biochemistry or related areas from any institution of higher education.Modern and important techniques are offered as possible support to help the student’s projects.2. Summer courses for undergraduate studentsThe Department offers every year, since 1999, complementary courses for undergraduate students to extend their knowledge in biochemical subjects not ordinarily treated in introductory courses. Some examples:Plant Molecular Biology, Biochemistry and Diseases, Biochemistry of Mind, Biochemistry of Ageing, Cancer Biochemistry, Nutrition and Sports, Biochemistry of Beauty, Biochemistry of the Envenomation Response, etc.3. Summer courses for high school teachers. Some examples:Biochemistry of Nutrition, DNA – Techniques and Applications, Biochemistry in the kitchen.4. Software developmentMany software for biochemistry teaching/learning were developed and are freely available at the Biblioteca Digital de Ciências [http://www.bdc.ib.unicamp.br/bdc/index.php]. Some examples:Oxygen consumption by mitochondria, Muscle contraction, Electron transport chain and oxidative phosphorylation, Free radicals, Enzyme kinetics, cAMP signalization, Interactive study of protein structure, Leptin, Insulin and Obesity.5. A Biochemistry textbook. 

  20. An Undergraduate Laboratory Experiment for Upper-Level Forensic Science, Biochemistry, or Molecular Biology Courses: Human DNA Amplification Using STR Single Locus Primers by Real-Time PCR with SYBR Green Detection

    Science.gov (United States)

    Elkins, Kelly M.; Kadunc, Raelynn E.

    2012-01-01

    In this laboratory experiment, real-time polymerase chain reaction (real-time PCR) was conducted using published human TPOX single-locus DNA primers for validation and various student-designed short tandem repeat (STR) primers for Combined DNA Index System (CODIS) loci. SYBR Green was used to detect the amplification of the expected amplicons. The…

  1. Redox biochemistry of hydrogen sulfide.

    Science.gov (United States)

    Kabil, Omer; Banerjee, Ruma

    2010-07-16

    H(2)S, the most recently discovered gasotransmitter, might in fact be the evolutionary matriarch of this family, being both ancient and highly reduced. Disruption of gamma-cystathionase in mice leads to cardiovascular dysfunction and marked hypertension, suggesting a key role for this enzyme in H(2)S production in the vasculature. However, patients with inherited deficiency in gamma-cystathionase apparently do not present vascular pathology. A mitochondrial pathway disposes sulfide and couples it to oxidative phosphorylation while also exposing cytochrome c oxidase to this metabolic poison. This report focuses on the biochemistry of H(2)S biogenesis and clearance, on the molecular mechanisms of its action, and on its varied biological effects.

  2. CLSI-Derived Hematology and Biochemistry Reference Intervals for Healthy Adults in Eastern and Southern Africa

    Science.gov (United States)

    Karita, Etienne; Ketter, Nzeera; Price, Matt A.; Kayitenkore, Kayitesi; Kaleebu, Pontiano; Nanvubya, Annet; Anzala, Omu; Jaoko, Walter; Mutua, Gaudensia; Ruzagira, Eugene; Mulenga, Joseph; Sanders, Eduard J.; Mwangome, Mary; Allen, Susan; Bwanika, Agnes; Bahemuka, Ubaldo; Awuondo, Ken; Omosa, Gloria; Farah, Bashir; Amornkul, Pauli; Birungi, Josephine; Yates, Sarah; Stoll-Johnson, Lisa; Gilmour, Jill; Stevens, Gwynn; Shutes, Erin; Manigart, Olivier; Hughes, Peter; Dally, Len; Scott, Janet; Stevens, Wendy; Fast, Pat; Kamali, Anatoli

    2009-01-01

    Background Clinical laboratory reference intervals have not been established in many African countries, and non-local intervals are commonly used in clinical trials to screen and monitor adverse events (AEs) among African participants. Using laboratory reference intervals derived from other populations excludes potential trial volunteers in Africa and makes AE assessment challenging. The objective of this study was to establish clinical laboratory reference intervals for 25 hematology, immunology and biochemistry values among healthy African adults typical of those who might join a clinical trial. Methods and Findings Equal proportions of men and women were invited to participate in a cross sectional study at seven clinical centers (Kigali, Rwanda; Masaka and Entebbe, Uganda; two in Nairobi and one in Kilifi, Kenya; and Lusaka, Zambia). All laboratories used hematology, immunology and biochemistry analyzers validated by an independent clinical laboratory. Clinical and Laboratory Standards Institute guidelines were followed to create study consensus intervals. For comparison, AE grading criteria published by the U.S. National Institute of Allergy and Infectious Diseases Division of AIDS (DAIDS) and other U.S. reference intervals were used. 2,990 potential volunteers were screened, and 2,105 (1,083 men and 1,022 women) were included in the analysis. While some significant gender and regional differences were observed, creating consensus African study intervals from the complete data was possible for 18 of the 25 analytes. Compared to reference intervals from the U.S., we found lower hematocrit and hemoglobin levels, particularly among women, lower white blood cell and neutrophil counts, and lower amylase. Both genders had elevated eosinophil counts, immunoglobulin G, total and direct bilirubin, lactate dehydrogenase and creatine phosphokinase, the latter being more pronounced among women. When graded against U.S.-derived DAIDS AE grading criteria, we observed 774 (35

  3. CLSI-derived hematology and biochemistry reference intervals for healthy adults in eastern and southern Africa.

    Directory of Open Access Journals (Sweden)

    Etienne Karita

    Full Text Available BACKGROUND: Clinical laboratory reference intervals have not been established in many African countries, and non-local intervals are commonly used in clinical trials to screen and monitor adverse events (AEs among African participants. Using laboratory reference intervals derived from other populations excludes potential trial volunteers in Africa and makes AE assessment challenging. The objective of this study was to establish clinical laboratory reference intervals for 25 hematology, immunology and biochemistry values among healthy African adults typical of those who might join a clinical trial. METHODS AND FINDINGS: Equal proportions of men and women were invited to participate in a cross sectional study at seven clinical centers (Kigali, Rwanda; Masaka and Entebbe, Uganda; two in Nairobi and one in Kilifi, Kenya; and Lusaka, Zambia. All laboratories used hematology, immunology and biochemistry analyzers validated by an independent clinical laboratory. Clinical and Laboratory Standards Institute guidelines were followed to create study consensus intervals. For comparison, AE grading criteria published by the U.S. National Institute of Allergy and Infectious Diseases Division of AIDS (DAIDS and other U.S. reference intervals were used. 2,990 potential volunteers were screened, and 2,105 (1,083 men and 1,022 women were included in the analysis. While some significant gender and regional differences were observed, creating consensus African study intervals from the complete data was possible for 18 of the 25 analytes. Compared to reference intervals from the U.S., we found lower hematocrit and hemoglobin levels, particularly among women, lower white blood cell and neutrophil counts, and lower amylase. Both genders had elevated eosinophil counts, immunoglobulin G, total and direct bilirubin, lactate dehydrogenase and creatine phosphokinase, the latter being more pronounced among women. When graded against U.S. -derived DAIDS AE grading criteria

  4. Searching for Alien Life Having Unearthly Biochemistry

    Science.gov (United States)

    Jones, Harry

    2003-01-01

    The search for alien life in the solar system should include exploring unearth-like environments for life having an unearthly biochemistry. We expect alien life to conform to the same basic chemical and ecological constraints as terrestrial life, since inorganic chemistry and the laws of ecosystems appear to be universal. Astrobiologists usually assume alien life will use familiar terrestrial biochemistry and therefore hope to find alien life by searching near water or by supplying hydrocarbons. The assumption that alien life is likely to be based on carbon and water is traditional and plausible. It justifies high priority for missions to search for alien life on Mars and Europa, but it unduly restricts the search for alien life. Terrestrial carbon-water biochemistry is not possible on most of the bodies of our solar system, but all alien life is not necessarily based on terrestrial biochemistry. If alien life has a separate origin from Earth life, and if can survive in an environment extremely different from Earth's, then alien life may have unearthly biochemistry. There may be other solvents than water that support alien life and other elements than carbon that form complex life enabling chain molecules. Rather than making the exploration-restricting assumption that all life requires carbon, water, and terrestrial biochemistry, we should make the exploration-friendly assumption that indigenous, environmentally adapted, alien life forms might flourish using unearthly biochemistry in many places in the solar system. Alien life might be found wherever there is free energy and a physical/chemical system capable of using that energy to build living structures. Alien life may be discovered by the detection of some general non-equilibrium chemistry rather than of terrestrial biochemistry. We should explore all the potential abodes of life in the solar system, including those where life based on terrestrial biochemistry can not exist.

  5. In memory of Professor Leonor Michaelis in Nagoya: great contributions to biochemistry in Japan in the first half of the 20th century.

    Science.gov (United States)

    Nagatsu, Toshiharu Toshi

    2013-09-02

    Leonor Michaelis spent the years of 1922-1926 as Professor of Biochemistry of the Aichi Medical College (now Graduate School of Medicine, Nagoya University) in Nagoya, Japan. Michaelis succeeded in gathering many bright young biochemists from all over Japan into his laboratory, and made tremendous contributions to the promotion of biochemistry in Japan. Michaelis was invited to many places in Japan to present lectures over those years. Kunio Yagi, who was Professor of Biochemistry at Nagoya University in the second half of the 20th century, succeeded in crystallizing the "Michaelis" enzyme-substrate complex. Historically, Michelis has had an enormous impact on biochemistry in Japan.

  6. Evaluation of the use of five laboratory determined ozone absorption cross sections in brewer and dobson retrieval algorithms

    Directory of Open Access Journals (Sweden)

    A. Redondas

    2013-09-01

    Full Text Available The primary ground-based instruments used to report total column ozone (TOC are Brewer and Dobson Spectrophotometers, in separate networks. These instruments make measurements of the UV irradiances, and through a well-defined process a TOC value is produced. Inherent in the algorithm is the use of a laboratory determined cross-section data set. We used five ozone cross section data sets: three Bass and Paur, Daumont, Malicet and Brion (DMB and a new Institute of Environmental Physics (IUP, University of Bremen, set. The three Bass and Paur (1985 sets are: quadratic temperature coefficients from IGACO web page (IGQ4, the Brewer network operational calibration set (BOp, and the set used by Bernhard et al. (2005, in the reanalysis of the Dobson absorption coefficient values (B05. The ozone absorption coefficients for Brewer and Dobson are then calculated using the normal Brewer operative method which is essentially the same as used on Dobson. Considering the standard TOC algorithm for the Brewer instruments and comparing to the Brewer standard operational calibration data set, using the slit functions for the individual instruments: we find the UIP data set changes the calculated TOC by −0.5%, the DBM data set changes the calculate TOC by −3.2%, and the IGQ4 data set at −45 °C changes the calculated TOC by +1.3%. Considering the standard algorithm for the Dobson instruments, and comparing to results using the official 1992 ozone absorption coefficients values and the single set of slit functions defined for all Dobson instruments, the calculated TOC changes by +1%, with little variation depending on which data set is used We applied the changes to the European Dobson and Brewer reference instruments during the Izaña 2012 Absolute Calibration Campaign. The application of a common Langley calibration and the IUP cross section the differences between Brewer and Dobson vanish whereas using Bass and Paur and DBM produce differences of 1.5% and 2

  7. Useful Demonstrations for a Medial Biochemistry Course.

    Science.gov (United States)

    Ragatz, Barth H.; Modrak, Gina

    1986-01-01

    Describes six demonstrations used in a medical biochemistry course. These demonstrations focus on: (1) platelet aggregometry; (2) ion-transporting antibiotics; (3) glycosylated hemoglobin; (4) molecular models; (5) serum preparation; and (6) bioluminescence. (JN)

  8. Preparative Protein Production from Inclusion Bodies and Crystallization: A Seven-Week Biochemistry Sequence

    Science.gov (United States)

    Peterson, Megan J.; Snyder, W. Kalani; Westerman, Shelley; McFarland, Benjamin J.

    2011-01-01

    We describe how to produce and purify proteins from "Escherichia coli" inclusion bodies by adapting versatile, preparative-scale techniques to the undergraduate laboratory schedule. This 7-week sequence of experiments fits into an annual cycle of research activity in biochemistry courses. Recombinant proteins are expressed as inclusion bodies,…

  9. Comprehensive Experiment--Clinical Biochemistry: Determination of Blood Glucose and Triglycerides in Normal and Diabetic Rats

    Science.gov (United States)

    Jiao, Li; Xiujuan, Shi; Juan, Wang; Song, Jia; Lei, Xu; Guotong, Xu; Lixia, Lu

    2015-01-01

    For second year medical students, we redesigned an original laboratory experiment and developed a combined research-teaching clinical biochemistry experiment. Using an established diabetic rat model to detect blood glucose and triglycerides, the students participate in the entire experimental process, which is not normally experienced during a…

  10. Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (edited by Gerhard Michal)

    Science.gov (United States)

    Voige, Reviewed By William H.

    2000-02-01

    For decades, a wall chart detailing living organisms' metabolic pathways has been a fixture in many classrooms and laboratories where biochemistry is taught. One of the most popular of those charts first appeared 30 years ago. Now its editor, Gerhard Michal, has produced a book that summarizes metabolism (broadly defined) in graphical and textual formats. The book retains the elegance of the chart. Names of molecules are printed in a crisp, easy-to-read font, and structural formulas are shown with exemplary clarity. Color coding serves multiple purposes: to differentiate enzymes, substrates, cofactors, and effector molecules; to indicate in which group or groups of organisms a reaction has been observed; and to distinguish enzymatic reactions from regulatory effects. The primary advantage of presenting this information in book format is immediately apparent. A typical metabolic chart covers about 2 m2; the book has a total surface area nearly 10 times greater. The extra space is used to add explanatory text to the figures and to include many topics not covered by the traditional definition of metabolism. Examples include replication, transcription, translation, reaction mechanisms for proteolytic enzymes, and the role of chaperones in protein folding. Illustrating these topics is not as straightforward as delineating a metabolic pathway, but the author has done an admirable job of designing figures that clarify these and other aspects of biochemistry and complement the accompanying text. A potential deficiency of book format is the inability to clearly show links between different realms of metabolism: carbohydrate and amino acid pathways, for example. The book overcomes this problem in two ways. A diagrammatic overview of metabolism (with references to applicable sections of the book) is printed inside its front cover, and key compounds (pyruvate, for example) have a distinctive green background to provide a visual link between pathways. (The author compares this

  11. CSEWG SYMPOSIUM, A CSWEG RETROSPECTIVE. 35TH ANNIVERSARY CROSS SECTION EVALUATION WORKING GROUP, NOV. 5, 2001, BROOKHAVEN NATIONAL LABORATORY.

    Energy Technology Data Exchange (ETDEWEB)

    DUNFORD, C.; HOLDEN, N.; PEARLSTEIN, S.

    2001-11-05

    This publication has been prepared to record some of the history of the Cross Section Evaluation Working Group (CSEWG). CSEWG is responsible for creating the evaluated nuclear data file (ENDF/B) which is widely used by scientists and engineers who are involved in the development and maintenance of applied nuclear technologies. This organization has become the model for the development of nuclear data libraries throughout the world. The data format (ENDF) has been adopted as the international standard. On November 5, 2001, a symposium was held at Brookhaven National Laboratory to celebrate the 50 th meeting of the CSEWG organization and the 35 th anniversary of its first meeting in November 1966. The papers presented in this volume were prepared by present and former CSEWG members for presentation at the November 2001 symposium. All but two of the presentations are included. I have included an appendix to list all of the CSEWG members and their affiliations, which has been compiled from the minutes of each of the CSEWG meetings. Minutes exist for all meetings except the 4 th meeting held in January 1968. The list includes 348 individuals from 71 organizations. The dates for each of the 50 CSEWG meetings are listed. The committee structure and chairmen of all committees and subcommittees are also included in the appendix. This volume is dedicated to three individuals whose foresight and talents made CSEWG possible and successful. They are Henry Honeck who lead the effort to develop the ENDF format and the CSEWG system, Ira Zartman, the Atomic Energy Commission program manager who provided the programmatic direction and support, and Sol Pearlstein who led the development of the CESWG organization and the ENDF/B evaluated nuclear data library.

  12. Combining content and elements of communication into an upper-level biochemistry course.

    Science.gov (United States)

    Whittington, Carli P; Pellock, Samuel J; Cunningham, Rebecca L; Cox, James R

    2014-01-01

    This report describes how a science communication module was incorporated into an advanced biochemistry course. Elements of communication were taught synergistically with biochemistry content in this course in an effort to expose students to a variety of effective oral communication strategies. Students were trained to use these established techniques and incorporated them into various presentations throughout the course. Three students describe their use of specific resources and how the skills learned relate to their future career. The importance and relevance of science communication are receiving unprecedented national attention. The academic scientific community must respond by incorporating more communication-centered instruction and opportunities in the classroom and laboratory.

  13. The Biochemistry Tetrahedron and the Development of the Taxonomy of Biochemistry External Representations (TOBER)

    Science.gov (United States)

    Towns, Marcy H.; Raker, Jeffrey R.; Becker, Nicole; Harle, Marissa; Sutcliffe, Jonathan

    2012-01-01

    Visual literacy, the ability to interpret and create external representations (ERs), is essential to success in biochemistry. Studies have been conducted that describe students' abilities to use and interpret specific types of ERs. However, a framework for describing ERs derived through a naturalistic inquiry of biochemistry classrooms has not…

  14. BIOLUMINESCENCE: TEACHING BIOCHEMISTRY BEYOND THE UNIVERSITY WALLS

    Directory of Open Access Journals (Sweden)

    Ana Paula Jesus de Almeida

    2016-11-01

    Full Text Available INTRODUCTION: The use of video in teaching and learning processes provides a challenging environment, able to stimulate the intellect and facilitate understanding in life science studies. Videos can be of extraordinary importance in education and dissemination of knowledge, contributing to greater learning, but is rarely used and exploited properly, especially for teaching biochemistry. Biochemistry is considered complex because it involves many molecular structures and processes, especially considering the number of events and molecules involved in the metabolism. OBJECTIVES: This study aimed to introduce biochemistry for the students of basic education using the theme "Light, Science and Life" in a playful and fun way. MATERIALS AND METHODS: A video about bioluminescence was designed and prepared aiming to use it as a support for learning biochemistry by students of basic education of public schools located in Salvador, Bahia. In order to prepare the video, undergraduate students initially revised the literature in order to acquire proper knowledge, and along with their teacher advisor worked the elaboration of texts, textbook and questionnaire and applied at school. DISCUSSION AND RESULTS: Analysis the qualitative results of the experiment on the preparation and use of the video about "Bioluminescence" focused mainly on the content of biochemistry linked to theme Light, Science and Life, and demonstrated the importance of such work in the teaching-learning process. The dynamics used allowed greater interaction between students and teacher, and the teaching of biochemistry in a fun way beyond the university walls. CONCLUSION: The teaching through recreational resources, e.g. videos and other educational strategies that foster learning should be encouraged from basic education, always bearing in order to transmit through these teaching methods the main concepts covered in biochemistry.

  15. THE BACHELOR OF BIOCHEMISTRY IN BRAZIL

    Directory of Open Access Journals (Sweden)

    H. G. Cordeiro

    2015-08-01

    Full Text Available INTRODUCTION: The economic and social development of Brazil in the last decade has contributed to the installation of new graduate and undergraduate programs, as are the case with bachelor degrees in Biochemistry at UFV, UFSJ and UEM. These graduates are prepared to work in industry, research institutes and universities in areas of knowledge involving Biochemistry and Molecular Biology. This is happening in developed countries since the first half of the last century, surprising and late is the implementation of bachelor of Biochemistry in Brazil. OBJECTIVES: The aim of this study was to perform a comparative analysis of the Bachelor in Biochemistry in Brazil from the perspective of the main difficulties of implementing and courses maintenance. MATERIAL AND METHODS: This is a descriptive research with a qualitative approach. Interviews were conducted with undergraduate students, graduates, professors, staff and managers from bachelor degrees in Biochemistry at UFV, UFSJ and UEM. The sampling procedure was non probabilistic for judgment (choice of the subjects involved and interested in the course of biochemistry to undergraduate students, graduates, professors and staff and non probabilistic for convenience to managers. The qualitative assessment to depict the representative keywords was performed using words cloud by WordleTM. RESULTS: The study included 5 managers, 24 professors, 12 staff, 25 undergraduate students, 5 graduates. For the students the main reason for the course selection should be scientific vocation and affinity with chemistry and biology; most cited positive parameters were faculty and great structure, practical classes and broad playing field; most cited negatives were high mandatory disciplines, little student free time and lack of sophisticated equipment. Despite the conception of the programs have happened in different contexts and regions we noted similarities deficiencies and distresses. CONCLUSION: Difficulties must be

  16. Effects of intensive mariculture on sediment biochemistry

    DEFF Research Database (Denmark)

    Pusceddu, Antonio; Fraschetti, Simonetta; Mirto, Simone

    2007-01-01

    The exponential growth of off-shore mariculture that has occurred worldwide over the last 10 years has raised concern about the impact of the waste produced by this industry on the ecological integrity of the sea bottom. Investigations into this potential source of impact on the biochemistry...... of the sea floor have provided contrasting results, and no compelling explanations for these discrepancies have been provided to date. To quantify the impact of fish-farm activities on the biochemistry of sediments, we have investigated the quantity and biochemical composition of sediment organic matter...... regions, with the exception of seagrass sediments in Spain, the biochemistry of the sediments showed significant differences between the control and fish-farm locations. However, the variables explaining the differences observed varied among the regions and between habitats, suggesting idiosyncratic...

  17. Ethanol Metabolism and the Transition from Organic Chemistry to Biochemistry

    Science.gov (United States)

    Feinman, Richard D.

    2001-09-01

    To ease the transition from organic chemistry at the beginning of a biochemistry course or at the beginning of the metabolism section of the organic course, an early presentation of the oxidation of ethanol is proposed. Alcohol dehydrogenase and aldehyde dehydrogenase reactions can smooth the introduction to biochemistry, since they involve three of the simplest compounds: ethanol, acetaldehyde, and acetic acid. Using these reactions as a model encourages the study of metabolic pathways by a systematic approach rather than by rote memorization. Reactions that can be presented as variations on a theme include methanol poisoning, the polyol reaction, and, most important, the sequence glycerol-3-phosphate to glyceraldehyde-3-phosphate to 3-phosphoglyceric acid. This last sequence integrates lipid and carbohydrate metabolism and, by comparison with the model reaction, brings out the principles of substrate-level phosphorylation. The method has evoked favorable verbal feedback from students and, in addition to medical and graduate courses, has been successfully used in the biochemical section of an undergraduate organic course.

  18. Commentary: PhDs in biochemistry education-5 years later.

    Science.gov (United States)

    Offerdahl, Erika G; Momsen, Jennifer L; Osgood, Marcy

    2014-01-01

    In this commentary, the discussion of PhDs in biochemistry education research is expanded to explore a number of diverse pathways leading to a competitive research program in biochemistry education research.

  19. Commentary: PhDs in Biochemistry Education--5 Years Later

    Science.gov (United States)

    Offerdahl, Erika G.; Momsen, Jennifer L.; Osgood, Marcy

    2014-01-01

    In this commentary, the discussion of PhDs in biochemistry education research is expanded to explore a number of diverse pathways leading to a competitive research program in biochemistry education research.

  20. Biochemistry in Biology and Chemistry Courses.

    Science.gov (United States)

    Wood, E. J.

    1987-01-01

    Discusses the state of biochemistry education in the United Kingdom. Gives an overview of the current educational system. Lists the problems of the school children, the school teachers, and the university teachers and emphasizes problems in the school syllabi. Suggests solutions to these problems. (CW)

  1. Identification of Threshold Concepts for Biochemistry

    Science.gov (United States)

    Loertscher, Jennifer; Green, David; Lewis, Jennifer E.; Lin, Sara; Minderhout, Vicky

    2014-01-01

    Threshold concepts (TCs) are concepts that, when mastered, represent a transformed understanding of a discipline without which the learner cannot progress. We have undertaken a process involving more than 75 faculty members and 50 undergraduate students to identify a working list of TCs for biochemistry. The process of identifying TCs for…

  2. Jmol-Enhanced Biochemistry Research Projects

    Science.gov (United States)

    Saderholm, Matthew; Reynolds, Anthony

    2011-01-01

    We developed a protein research project for a one-semester biochemistry lecture class to enhance learning and more effectively train students to understand protein structure and function. During this semester-long process, students select a protein with known structure and then research its structure, sequence, and function. This project…

  3. A Biochemistry and Molecular Biology Course for Secondary School Teachers

    Science.gov (United States)

    Fernandez-Novell, J. M.; Cid, E.; Gomis, R.; Barbera, A.; Guinovart, J. J.

    2004-01-01

    This article describes a course for reinforcing the knowledge of biochemistry in secondary school science teachers. The Department of Biochemistry and Molecular Biology of the University of Barcelona designed a course to bring these teachers up to date with this discipline. In addition to updating their knowledge of biochemistry and molecular…

  4. Television Medical Dramas as Case Studies in Biochemistry

    Science.gov (United States)

    Millard, Julie T.

    2009-01-01

    Several case studies from popular television medical dramas are described for use in an undergraduate biochemistry course. These cases, which illustrate fundamental principles of biochemistry, are used as the basis for problems that can be discussed further in small groups. Medical cases provide an interesting context for biochemistry with video…

  5. Biochemistry in the idea of graduation students

    Directory of Open Access Journals (Sweden)

    D. F. Escoto et al

    2015-08-01

    Full Text Available INTRODUCTION AND OBJECTIVE: Biochemistry is an interdisciplinary area that allows us to study chemical phenomena in live organisms. That way, its study is of extreme importance, in all levels, to enlarge the comprehension of natural phenomena. However, it is barely explored in the basic education and often fragmented in the higher education, or in graduation degrees that contemplate this area. Especially in the teacher training, where the fragmentation of knowledge can contribute to form wrong concepts. Based on that, this work aims to identify the concept of Biochemistry according to the future teachers of Natural Science. MATERIALS AND METHODS: The work was developed with 3º, 5º and 9º semesters students of the natural science degree on Universidade Federal do Pampa. 50 students, from 18 to 56 years old, were interviewed. The data was obtained through a semi-structured questionnaire. The methodology of categorization and analysis of content with emergent categories of speech was chosen for the analysis. RESULTS AND CONCLUSION: Initially, 11 categories were chosen by content similarity. In descending order: chemical reactions in organisms, chemistry area, chemistry of life, cell metabolism, the study of living beings, origin of life, biology area, organic balance, chemical-biological study. The reports made possible to identify that most students do understand with clarity the goal of studying biochemistry. Although, we can see that there are some students that fragment the area, what means, they try to discriminate chemistry from biology. This way, they demonstrate a difficulty to comprehend biochemistry as interdisciplinary, what makes it hard to contextualize the built knowledge. It is important to develop strategies to overcome the fragmentation of knowledge, so that biochemistry can be comprehended in its fullness and help on the teaching processes that will be developed by the future teachers.

  6. Laboratory Accreditation in Argentina

    Science.gov (United States)

    Collino, Cesar; Chiabrando, Gustavo A.

    2015-01-01

    Laboratory accreditation is an essential element in the healthcare system since it contributes substantially to decision-making, in the prevention, diagnosis, treatment and follow-up of the health status of the patients, as well as in the organization and management of public healthcare. Therefore, the clinical biochemistry professional works continuously to provide reliable results and contributes to the optimization of operational logistics and integration of a laboratory into the health system. ISO 15189 accreditation, ensures compliance of the laboratory to minimize instances of error through the planning, prevention, implementation, evaluation and improvement of its procedures, which provides skill areas that involve both training undergraduate and graduate professionals in clinical biochemistry. PMID:27683497

  7. Report style guide for subcontractors of the Efficiency and Renewables Research Section, Energy Division, Oak Ridge National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Stone, T.A.; Bennett, M.N.

    1992-09-01

    This document has been paraphrased from the ORNL Document Preparation Guide (DPG). It is intended for use by Efficiency and Renewables Research Section, Energy Division, ORNL subcontractor reports so that review and editing effort can be minimized. Topics covered are typing instructions, document format, usage, abbreviations and acronyms, and standard editing marks.

  8. A laboratory study of Fe XVII x-ray excitation cross sections using the LLNL EBIT facility and NASA/GSFC AstroE microcalorimeters

    Science.gov (United States)

    Brown, G. V.; Beiersdorfer, P.; Boyce, K. R.; Chen, H.; Kahn, S. M.; Kelley, R. L.; Kilbourne, C. A.; Porter, F. S.; Szymkowiak, A.

    2004-08-01

    The mixing of atomic and macroscopic processes taking place in non-terrestrial objects creates complex, dynamic, and intriguing environments. High-resolution x-ray spectra from these sources measured by satellites such as the Chandra X-ray Observatory, XMM-Newton, and the Solar Maximum Mission provide a means for understanding the physics governing these sources. Laboratory measurements of the atomic processes have proved crucial to the interpretation of these spectra. For example, using the LLNL electron beam ion traps EBIT-I & EBHIT-II a detailed study of the x-ray spectrum of Fe xvii has been conducted addressing the large ratio predicted by theory compared to observations for the relative intensity of the 2p-3d 1P1 resonance to 3D1 intercombination line. The difference was often attributed to opacity effects. However, laboratory measurements in the optically thin limit agree with observations demonstrating that the prediction is too large. The laboratory results thus provide a benchmark in the optically thin limit for accurate estimates of opacity effects. To uncover the source of the discrepancy between theory and observation, we have performed a series of experiments that successively uncovered more details about the Fe XVII lines produced in coronal plasmas. Most recently, we used NASA's 32 channel array microcalorimeter from the Astro-E x-ray satellite program to measure the excitation cross section of various Fe XVII lines in the laboratory. Our results show that the theoretically predicted cross section for the resonance line is too large while the value for the intercombination line is correct. These measurements resolve long-standing issues thought to be associated with non-equilibrium processes. Work at LLNL was completed under the auspices of the U.S. D.o.E by the University of California Lawrence Livermore National Laboratory under contract W-7405-Eng-48 and supported by NASA's Astronomy and Physics Research and Analysis Program under work order S

  9. Virtual Practice of Biochemistry: Ascendant Chromatography on Paper of Amino acids.

    OpenAIRE

    Zanatta,G.; De Moraes, C G; E.R. Zimmer; E. Ewald; Pettenuzzo,L.F.; Salbego, C. G.; V.M.T. Trindade

    2010-01-01

    Chromatography is a method used for analyses of mixture components based on different criteria: adsorbility, solubility, molecular mass, ionic charge and affinity. It is an important subject of Biochemistry. This work reports the development and the validation of a software that simulated a laboratory activity named Ascendant Chromatography on Paper of Aminoacids. The organization and the multimedia material collection were done during the 2008/2 semester. The most representative images were ...

  10. Pracital examination in biochemistry: topics and procedures?

    Directory of Open Access Journals (Sweden)

    Vocke, Nils-Daniel

    2005-08-01

    Full Text Available Under the new revision of the German licensing regulations for physicians ("Approbationsordnung" the rules for the first national examinations (i.e. "Physikum", equivalent to medical school entrance exams have been changed. In addition to the written exam a novel "oral-practical" part is required. Here we describe the implications of this oral-practical exam on the examination procedures in the field of biochemistry/molecular biology. A strategy for its realization is proposed.

  11. A Phytase Enzyme-Based Biochemistry Practical Particularly Suited to Students Undertaking Courses in Biotechnology and Environmental Science

    Science.gov (United States)

    Boyce, Angela; Casey, Anne; Walsh, Gary

    2004-01-01

    Courses in introductory biochemistry invariably encompass basic principles of enzymology, with reinforcement of lecture-based material in appropriate laboratory practicals. Students undertaking practical classes are more enthusiastic, and generally display improved performance, when the specific experiments undertaken show direct relevance to…

  12. Two-Dimensional Nuclear Magnetic Resonance Structure Determination Module for Introductory Biochemistry: Synthesis and Structural Characterization of Lyso-Glycerophospholipids

    Science.gov (United States)

    Garrett, Teresa A.; Rose, Rebecca L.; Bell, Sidney M.

    2013-01-01

    In this laboratory module, introductory biochemistry students are exposed to two-dimensional [superscript 1]H-nuclear magnetic resonance of glycerophospholipids (GPLs). Working in groups of three, students enzymatically synthesized and purified a variety of 2-acyl lyso GPLs. The structure of the 2-acyl lyso GPL was verified using [superscript…

  13. 1 MA Breech Upgrade for the U.S. Army Research Laboratory’s (ARL’s) Rectangular 22- x-44-mm In-bore Cross Section Railgun

    Science.gov (United States)

    2011-08-01

    60-μH or a 30-μH inductor, allowed the generation of output pulses of different profiles. Since 2001, the power supply, railgun and breech, has...A Spice-based Code for ARL’s 4.5-MJ Electromagnetic Launcher Pulsed Power Supply System; ARL-TR- 2592; U.S. Army Research Laboratory: Aberdeen...1 MA Breech Upgrade for the U.S. Army Research Laboratory’s (ARL’s) Rectangular 22- × 44-mm In-bore Cross Section Railgun by Miguel A. Del

  14. Elastic, charge transfer, and related transport cross sections for proton impact of atomic hydrogen for astrophysical and laboratory plasma modeling

    Science.gov (United States)

    Schultz, D. R.; Ovchinnikov, S. Yu; Stancil, P. C.; Zaman, T.

    2016-04-01

    Updating and extending previous work (Krstić and Schultz 1999 J. Phys. B: At. Mol. Opt. Phys. 32 3458 and other references) comprehensive calculations were performed for elastic scattering and charge transfer in proton—atomic hydrogen collisions. The results, obtained for 1301 collision energies in the center-of-mass energy range of 10-4-104 eV, are provided for integral and differential cross sections relevant to transport modeling in astrophysical and other plasma environments, and are made available through a website. Use of the data is demonstrated through a Monte Carlo transport simulation of solar wind proton propagation through atomic hydrogen gas representing a simple model of the solar wind interaction with heliospheric neutrals.

  15. The Alcohol Dehydrogenase Kinetics Laboratory: Enhanced Data Analysis and Student-Designed Mini-Projects

    Science.gov (United States)

    Silverstein, Todd P.

    2016-01-01

    A highly instructive, wide-ranging laboratory project in which students study the effects of various parameters on the enzymatic activity of alcohol dehydrogenase has been adapted for the upper-division biochemistry and physical biochemistry laboratory. Our two main goals were to provide enhanced data analysis, featuring nonlinear regression, and…

  16. A Changing Research and Publication Landscape for Biochemistry

    Directory of Open Access Journals (Sweden)

    Gabor Mocz

    2008-01-01

    Full Text Available This introductory editorial hopes to convey three points to its audience. First, it provides an overview of the new, peer-reviewed, open access journal Biochemistry Insights published by Libertas Academica. Second, it summarizes the benefits of open access publishing concepts to the biochemistry community. And third, it takes a brief look at the near future of biochemistry as a fundamental molecular science whose continued advances and latest developments will be the focus of the new journal. Biochemistry Insights looks forward to receiving research articles, review papers, commentaries and letters from all disciplines and specialties of the field.

  17. BIOCHEMISTRY TEACHING WITH VIRTUAL DYNAMIC METABOLIC DIAGRAMS

    Directory of Open Access Journals (Sweden)

    G. B. Lazzarotto

    2004-05-01

    Full Text Available This work presents a game like educational software (courseware to study metabolic pathways, calledDiagrama Metabolico Din^amico Virtual (DMDV of Krebs Cycle. The experience acquired teachingwith the logical sequence tray games in the FFFCMPAs Biochemistry Course provides the beddingswith the use of this model as education method. With DMDV, students can assembly the sequenceof reactions that describe the desired metabolic pathway, create situational models which can guidehis/her choices, reduce the subject complexity of the scheme in knowledge construction presentingin a graphical way the current interrelations. Biochemistry teachers can use the present software inclassroom as well as distance classes. This product integrates multimedia resources extensively andis distributed in CD-ROM format. The virtual environment will make possible interaction of thestudent with the environment and with colleagues and teachers, through tools as chats and forum.Experience with the use of this method was carried through with two distinct groups of students.The rst group was composed by 11 students, who were more familiar with the content and answereda specic questionnaire to previously evaluate the software. The second group was formed by 24students regularly registered in the FFFCMPAs Biochemistry Course, who used the software as astudy method. The rst group considered DMDV of easy and pleasant navigation. The knowledgeevaluation of the second group students was made by a written test and the analysis of three conceptualmaps constructed by each one of them: one map before initiating the study with the DMDV, thesecond just after the study and the third one two months later. Every conceptual maps producedafter DMDV method showed an expansion of valid concepts if compared with the rst maps. Simplevisual comparison of maps shows that new elements where added. All students who passed throughthe experiment reached a greater than ve grade in the subjects written

  18. Guidelines for the Design of Biochemistry Websites

    Directory of Open Access Journals (Sweden)

    R.M. Lima

    2014-08-01

    Full Text Available The internet is a means of communication that cannot be disregarded by contemporaryeducational systems. Public and private incentive policies aimed at the application ofdigital devices in the exchange of knowledge in and outside schools have beenimplemented in the last decades. However, risks posed by academic research on line arewidely acknowledged with regard to the quality of information (Lourenço, 2010. One of themain aspects of this issue is the selection of reliable and potentially educational pagesdeveloped for specific target audiences. This study has the objective of providingguidelines for those who intend to develop sites, wiki pages, and educational PBwikisfocused on Biochemistry, or even adapt existing ones. Considering the fact that there is nospecific legislation for the use of the Internet in Brazil, it is necessary to stimulate selfregulationof the sector in order to establish minimum quality standards, safety, andreliability of sites containing information in the educational area. Thus, it is necessary toestablish some parameters to help guiding research for educational purposes on theinternet. To be a potentially didactic Biochemistry page, one must follow this set oforientation: clearly establish the target audience and, then, define the contents the properway. Once the materials have been organized, establish, whenever possible and pertinent,links with references to professional and/ or research home pages, to ProfessionalAssociations in the Biochemistry field, among others. Link the page to an institutionalresearch program, such as International Virtual Education Network, a program developedby the Brazilian Distance Education Department. Finally, it is necessary to provideinteractive possibilities and dynamic images of the biochemical processes studied in classthat will justify and differentiate the page from the many existing ones. Such proceduresare essential to guarantee that online searches made for educational

  19. The Sweetness of Aspartame: A Biochemistry Lab for Health Science Chemistry Courses

    Science.gov (United States)

    Stein, Paul J.

    1997-09-01

    A laboratory exercise for Health Science Biochemistry students to study the effect of aspartame concentration on sweetness has been developed. The concentration dependence of the absorbance of aspartame at 257 nm is also studied. Data from all members of the class are averaged and plotted on the same graph as absorbance and taste rating vs. [aspartame]. The absorbance plot follows Beer's law while the taste rating plot displays the typical hyperbolic response of protein-ligand binding plots. This laboratory exercise illustrates the concept of binding saturation to students.

  20. Penta-coordinate phosphorous compounds and biochemistry

    Institute of Scientific and Technical Information of China (English)

    林长学; 李艳梅; 成昌梅; 韩波; 万荣; 冯亚兵; 赵玉芬

    2002-01-01

    The relationship between penta-coordinate phosphorus compounds and biochemistry is briefly reviewed. Some interesting phenomena such as peptide formation, ester formation, ester exchange on phosphorus and N to O migration occur at room temperature when the amino group of amino acid is associated with phosphoryl group. Serine or threonine in conjugate of nucleoside-amino acid could recognize different nucleobases. N-phosphoryl Histine and Ser-His dipeptide could cleavage nucleic acid, protein and ester in neutral medium. It is found that the above phenomena all undergo penta-coordinate intermediate of phosphorus atom, which is proposed as the key factor to determine their activities.

  1. Chemistry and biochemistry of dietary polyphenols.

    Science.gov (United States)

    Tsao, Rong

    2010-12-01

    Polyphenols are the biggest group of phytochemicals, and many of them have been found in plant-based foods. Polyphenol-rich diets have been linked to many health benefits. This paper is intended to review the chemistry and biochemistry of polyphenols as related to classification, extraction, separation and analytical methods, their occurrence and biosynthesis in plants, and the biological activities and implications in human health. The discussions are focused on important and most recent advances in the above aspects, and challenges are identified for future research.

  2. Penta-coordinate phosphorous compounds and biochemistry

    Institute of Scientific and Technical Information of China (English)

    林长学; 韩波; 赵玉芬; 万荣; 李艳梅; 冯亚兵; 成昌梅

    2002-01-01

    The relationship between penta-coordinate phosphorus compounds and biochemistry is briefly reviewed. Some interesting phenomena such as peptide formation, ester formation, ester exchange on phosphorus and N to O migration occur at room temperature when the amino group of amino acid is associated with phosphoryl group. Serine or threonine in conjugate of nucleo-side-amino acid could recognize different nucleobases. N-phosphoryl Histine and Ser-His dipep-tide could cleavage nucleic acid, protein and ester in neutral medium. It is found that the above phenomena all undergo penta-coordinate intermediate of phosphorus atom, which is proposed as the key factor to determine their activities.

  3. Writing throughout the Biochemistry Curriculum: Synergistic Inquiry-Based Writing Projects for Biochemistry Students

    Science.gov (United States)

    Mertz, Pamela; Streu, Craig

    2015-01-01

    This article describes a synergistic two-semester writing sequence for biochemistry courses. In the first semester, students select a putative protein and are tasked with researching their protein largely through bioinformatics resources. In the second semester, students develop original ideas and present them in the form of a research grant…

  4. 生化室检验结果审核人员医嘱平均审核时间、心理状况及医疗投诉率调查及分析%Investigation and analysis on the average checking time of biochemistry results,psychologic status of biochemical laboratory test result inspectors,and medical complaint rate

    Institute of Scientific and Technical Information of China (English)

    刘彦明; 肖洪广; 黄靖宇; 何凤屏; 王凯

    2016-01-01

    目的:了解生化室检验结果审核人员心理健康状况及医嘱平均审核时间与其心理健康状况、生化室医疗投诉率的关系。方法采用自行设计的检验科生化室医疗投诉情况调查表、生化室信息系统和工作量情况调查表、症状自评量表(SCL‐90)中国修订版对广东省17家二级甲等以上医院检验科生化室检验结果审核人员进行问卷调查。结果生化室检验结果审核人员心理健康状况不如全国常模;女性心理健康状况与男性比较差别不大,但女性在恐怖方面表现较差;主任技师、副主任技师、主管技师心理健康状况比较无明显差别,但初级技师在一些心理因子方面(如强迫症状、躯体化、焦虑、抑郁、恐怖)明显差于其他专业技术职务者;生化室医嘱审核时间在一定程度上与检验结果审核人员心理健康状况、生化室医疗投诉率有关。结论广东省17家二级甲等以上医院检验科生化室检验结果审核人员心理健康状况稍差于国内常模;不同性别、专业技术职务比较心理健康状况无明显差别,但初级检验技师心理健康状况较其他专业技术职务者稍差;医嘱平均审核时间在一定程度上与生化室检验结果审核人员心理健康状况、生化室医疗投诉率有关。%Objective To investigate the relationship among the average checking time of biochemistry results ,mental health of biochemistry results checkers and the rate of medical complains .Methods 258 biochemistry results checkers from 17 hospitals of Guangdong were tested by using symptom checklist(SCL‐90) ,biochemistry results checking time questionnaire and medical com‐plaints questionnaire .Results Compared with the national model data ,the mental health levels of biochemistry results checkers were worse relatively .The mental heath did not have gender difference ,however ,female checkers were significant worse than

  5. An Investigation of the 40Ar(n,p)40Cl Reaction Cross-Section below 50MeV at Crocker Nuclear Laboratory

    Science.gov (United States)

    Walsh, Nicholas Ian

    Large underground liquid argon detectors are poised to detect neutrinos from the next galactic supernova. Liquid argon detectors are uniquely sensitive to the electron neutrino, thus giving them the capability to detect neutronization neutrinos for the first time. One background that may mimic the signal of this low-energy neutrino interaction in argon is the beta-decay of Cl-40 which is produced in argon by a fast neutron reaction. Previous measurements of this 40Ar+n->40Cl+p reaction cross section exist only below 15 MeV and the measurements differ by a factor of two. Using the U.C. Davis Crocker Nuclear Laboratory neutron beam this cross-section is determined by fitting to a parametrized model for neutron energies up to 50 MeV. Neutrons at this facility are generated from mono-energetic protons impinging on a thick beryllium target. Then, the neutrons that pass through the collimator are measured by time-of-flight and a fast-neutron activation technique. Using the neutron fluxes generated from five different proton energies, including 50 MeV protons, the 40Ar(n,p)40Cl reaction cross section is measured by irradiating liquid argon in each beam and counting the subsequent gammas from the Cl-40 decay in a high-purity germanium detector.

  6. Laboratory-scale column study for remediation of TCE-contaminated aquifers using three-section controlled-release potassium permanganate barriers.

    Science.gov (United States)

    Yuan, Baoling; Li, Fei; Chen, Yanmei; Fu, Ming-Lai

    2013-05-01

    A laboratory-scale study with a sand column was designed to simulate trichloroethylene (TCE) pollution in the aquifer environment with three-section controlled-release potassium permanganate (CRP) barriers. The main objective of this study was to evaluate the feasibility of CRP barriers in remediation of TCE in aquifers in a long-term and controlled manner. CRP particles with a 1:3 molar ratio of KMnO4 to stearic acid showed the best controlled-release properties in pure water, and the theoretical release time was 138.5 days. The results of TCE removal in the test column indicated that complete removal efficiency of TCE in a sand column by three-section CRP barriers could be reached within 15 days. The molar ratio of KMnO4 to TCE in the three-section CRP barriers was 16:1, which was much lower than 82:1 as required when KMnO4 solution is used directly to achieve complete destruction of TCE. This result revealed that the efficiency of CRP for remediation of TCE was highly improved after encapsulation.

  7. Laboratory-scale column study for remediation of TCE-contaminated aquifers using three-section controlled-release potassium permanganate barriers

    Institute of Scientific and Technical Information of China (English)

    Baoling Yuan; Fei Li; Yanmei Chen; Ming-Lai Fu

    2013-01-01

    A laboratory-scale study with a sand column was designed to simulate trichloroethylene (TCE) pollution in the aquifer environment with three-section controlled-release potassium permanganate (CRP) barriers.The main objective of this study was to evaluate the feasibility of CRP barriers in remediation of TCE in aquifers in a long-term and controlled manner.CRP particles with a 1:3 molar ratio of KMnO4 to stearic acid showed the best controlled-release properties in pure water,and the theoretical release time was 138.5 days.The results of TCE removal in the test column indicated that complete removal efficiency of TCE in a sand column by three-section CRP barriers could be reached within 15 days.The molar ratio of KMnO4 to TCE in the three-section CRP barriers was 16:1,which was much lower than 82∶1 as required when KMnO4 solution is used directly to achieve complete destruction of TCE.This result revealed that the efficiency of CRP for remediation of TCE was highly improved after encapsulation.

  8. Comprehensive experiment-clinical biochemistry: determination of blood glucose and triglycerides in normal and diabetic rats.

    Science.gov (United States)

    Jiao, Li; Xiujuan, Shi; Juan, Wang; Song, Jia; Lei, Xu; Guotong, Xu; Lixia, Lu

    2015-01-01

    For second year medical students, we redesigned an original laboratory experiment and developed a combined research-teaching clinical biochemistry experiment. Using an established diabetic rat model to detect blood glucose and triglycerides, the students participate in the entire experimental process, which is not normally experienced during a standard clinical biochemistry exercise. The students are not only exposed to techniques and equipment but are also inspired to think more about the biochemical mechanisms of diseases. When linked with lecture topics about the metabolism of carbohydrates and lipids, the students obtain a better understanding of the relevance of abnormal metabolism in relation to diseases. Such understanding provides a solid foundation for the medical students' future research and for other clinical applications.

  9. Pharmacology and biochemistry undergraduate students'concern for a healthy diet and nutrition knowledge

    OpenAIRE

    Bernardes Spexoto, Maria Claudia [UNESP; Ferin, Giovana Garcia [UNESP; Duarte Bonini Campos, Juliana Alvares [UNESP

    2015-01-01

    Objective: To estimate the concern for a healthy diet and the nutrition knowledge of undergraduate students of a pharmacology and biochemistry program and their associations with the variables of interest.Methods: This cross-sectional study administered the Nutrition Knowledge Scale and the How is your diet? questionnaire to 381 students. The associations between concern for a healthy diet and nutrition knowledge and between these two factors and the demographic variables were measured by the...

  10. A National Comparison of Biochemistry and Molecular Biology Capstone Experiences

    Science.gov (United States)

    Aguanno, Ann; Mertz, Pamela; Martin, Debra; Bell, Ellis

    2015-01-01

    Recognizing the increasingly integrative nature of the molecular life sciences, the "American Society for Biochemistry and Molecular Biology" (ASBMB) recommends that Biochemistry and Molecular Biology (BMB) programs develop curricula based on concepts, content, topics, and expected student outcomes, rather than courses. To that end,…

  11. Blended Learning in Biochemistry Education: Analysis of Medical Students' Perceptions

    Science.gov (United States)

    Wardenski, Rosilaine de Fatima; de Espindola, Marina Bazzo; Struchiner, Miriam; Giannella, Tais Rabetti

    2012-01-01

    The objective of this study was to analyze first-year UFRJ medical students' perceptions about the implementation of a blended learning (BL) experience in their Biochemistry I course. During the first semester of 2009, three Biochemistry professors used the Constructore course management system to develop virtual learning environments (VLEs) for…

  12. Using Pamphlets to Teach Biochemistry: A Service-Learning Project

    Science.gov (United States)

    Harrison, Melinda A.; Dunbar, David; Lopatto, David

    2013-01-01

    A service-learning project appropriate for a biochemistry or advanced biochemistry course was designed and implemented. The project involved students partnering with a homeless shelter to design informational pamphlets to be displayed at the shelter for the clients' use. The pamphlet topics were based on diseases studied within the course.…

  13. Commentary: Biochemistry and Molecular Biology Educators Launch National Network

    Science.gov (United States)

    Bailey, Cheryl; Bell, Ellis; Johnson, Margaret; Mattos, Carla; Sears, Duane; White, Harold B.

    2010-01-01

    The American Society of Biochemistry and Molecular Biology (ASBMB) has launched an National Science Foundation (NSF)-funded 5 year project to support biochemistry and molecular biology educators learning what and how students learn. As a part of this initiative, hundreds of life scientists will plan and develop a rich central resource for…

  14. Teachers as learners in a cooperative learning biochemistry class.

    Science.gov (United States)

    Osgood, Marcy P; Mitchell, Steve M; Anderson, William L

    2005-11-01

    Upper level college students majoring in biochemistry at the University of New Mexico have the opportunity to participate in an advanced biochemistry course entitled "Biochemistry Education." This course introduces theories of teaching and learning, provides opportunities for participation in course organization, design, and assessment strategies, and requires practice in lecturing, exam writing, and grading. One component of this course required the biochemistry majors to act as educational assistants, leading problem-based learning sessions in a cooperative learning introductory survey biochemistry course for nonmajors. Problem-based learning scenarios used in this course were based on real-life biochemistry problems. As a result of their participation, the educational assistants increased their understanding of the biochemistry principles, gained an appreciation for the difficulty of the job of a "good teacher," developed new approaches to their own learning, and became more confident speakers. The participating biochemistry faculty were also positively affected by the collaborative approach they were attempting to model for the two sets of students and realized the benefits of truly cooperative team teaching.

  15. Enhanced Podcasts for Teaching Biochemistry to Veterinary Students

    Science.gov (United States)

    Gough, Kevin C.

    2011-01-01

    The teaching of biochemistry within medical disciplines presents certain challenges; firstly to relay a large body of complex facts and abstract concepts, and secondly to motivate students that this relatively difficult topic is worth their time to study. Here, nutrient biochemistry was taught within a multidisciplinary module as part of an…

  16. Role of tautomerism in RNA biochemistry.

    Science.gov (United States)

    Singh, Vipender; Fedeles, Bogdan I; Essigmann, John M

    2015-01-01

    Heterocyclic nucleic acid bases and their analogs can adopt multiple tautomeric forms due to the presence of multiple solvent-exchangeable protons. In DNA, spontaneous formation of minor tautomers has been speculated to contribute to mutagenic mispairings during DNA replication, whereas in RNA, minor tautomeric forms have been proposed to enhance the structural and functional diversity of RNA enzymes and aptamers. This review summarizes the role of tautomerism in RNA biochemistry, specifically focusing on the role of tautomerism in catalysis of small self-cleaving ribozymes and recognition of ligand analogs by riboswitches. Considering that the presence of multiple tautomers of nucleic acid bases is a rare occurrence, and that tautomers typically interconvert on a fast time scale, methods for studying rapid tautomerism in the context of nucleic acids under biologically relevant aqueous conditions are also discussed.

  17. Restriction Enzymes in Microbiology, Biotechnology and Biochemistry

    Directory of Open Access Journals (Sweden)

    Geoffrey G. Wilson

    2012-12-01

    Full Text Available Since their discovery in the nineteen-seventies, a collection of simple enzymes termed Type II restriction endonucleases, made by microbes to ward off viral infections, have transformed molecular biology, spawned the multi-billion dollar Biotechnology industry, and yielded fundamental insights into the biochemistry of life, health and disease. In this article we describe how these enzymes were discovered, and we review their properties, organizations and genetics. We summarize current ideas about the mechanism underlying their remarkable ability to recognize and bind to specific base pair sequences in DNA, and we discuss why these ideas might not be correct. We conclude by proposing an alternative explanation for sequence-recognition that resolves certain inconsistencies and provides, in our view, a more satisfactory account of the mechanism.

  18. Bone Biochemistry on the International Space Station

    Science.gov (United States)

    Smith, Scott M.; Heer, Martina; Zwart, Sara R.

    2016-01-01

    Bone biochemical measures provide valuable insight into the nature and time course of microgravity effects on bone during space flight, where imaging technology cannot be employed. Increased bone resorption is a hallmark of space flight, while markers of bone formation are typically unchanged or decreased. Recent studies (after the deployment to ISS of the advanced resistive exercise device, ARED), have documented that astronauts with good nutritional intake (e.g., maintenance of body mass), good vitamin D status, and exercise maintained bone mineral density. These data are encouraging, but crewmembers exercising on the ARED do have alterations in bone biochemistry, specifically, bone resorption is still increased above preflight levels, but bone formation is also significantly increased. While this bone remodeling raises questions about the strength of the resulting bone, however documents beneficial effects of nutrition and exercise in counteracting bone loss of space flight.

  19. Effects of spaceflight on rat humerus geometry, biomechanics, and biochemistry

    Science.gov (United States)

    Vailas, A. C.; Zernicke, R. F.; Grindeland, R. E.; Kaplansky, A.; Durnova, G. N.; Li, K. C.; Martinez, D. A.

    1990-01-01

    The effects of a 12.5-day spaceflight (Cosmos 1887 biosatellite) on the geometric, biomechanical, and biochemical characteristics of humeri of male specific pathogen-free rats were examined. Humeri of age-matched basal control, synchronous control, and vivarium control rats were contrasted with the flight bones to examine the influence of growth and space environment on bone development. Lack of humerus longitudinal growth occurred during the 12.5 days in spaceflight. In addition, the normal mid-diaphysial periosteal appositional growth was affected; compared with their controls, the spaceflight humeri had less cortical cross-sectional area, smaller periosteal circumferences, smaller anterior-posterior periosteal diameters, and smaller second moments of area with respect to the bending and nonbending axes. The flexural rigidity of the flight humeri was comparable to that of the younger basal control rats and significantly less than that of the synchronous and vivarium controls; the elastic moduli of all four groups, nonetheless, were not significantly different. Generally, the matrix biochemistry of the mid-diaphysial cross sections showed no differences among groups. Thus, the spaceflight differences in humeral mechanical strength and flexural rigidity were probably a result of the differences in humeral geometry rather than material properties.

  20. Dyslipidemias as generating issue in Biochemistry classes

    Directory of Open Access Journals (Sweden)

    R. M. Lima

    2015-08-01

    Full Text Available The traditional didactic model is based on the transmission of the teacher's encyclopedic knowledge. In this model, the teaching of Science aims at the transmission of dominant values, regarded as absolute truths. The teacher is seen is an expert on scientific contents who transmits them to students without motivating them, and without taking into consideration their previous ideas and life experience. This model contributes to the formation of professionals who accept those values uncritically. An effective approach to break up this traditional teaching model in Biochemistry is the use of a generating issue. A Generating Issue is the starting point to the knowledge construction process which, in turn, replaces traditional models. Thus, this study aimed at developing a lesson for a 12th grade class at IF Fluminense on the following content: alcohol, carboxylic acid, ester, and esterification reaction, using dyslipidemias as the Generating Issue. To verify the value of such methodology in Biochemistry classroom, data was collected by applying a questionnaire and images with texts produced by students. In addition, they had a class based on the methodology known as Three Pedagogical Moments, proposed by Delizoicov et al. (2007. Several didactic resources designed by the authors were used, such as slide presentation, tridimensional molecular models, and a roulette game named “Bioquimicados”, based on the Facebook game “Perguntados” ("Trivia Crack". After this, students developed more grounded scientific concepts, making use of terms common in scientific language. This suggests that the use of the Generating Issue in a lesson based on problematization, and supported by a ludic activity, provided a meaningful contribution to improve the students' understanding of the scientific content. This type of non-traditional class promotes greater student motivation, resulting in meaningful learning.

  1. Virtual Biochemistry – pH effect on enzyme activity

    Directory of Open Access Journals (Sweden)

    D.N. Heidrich

    2011-04-01

    Full Text Available Protocols of laboratory experiments, followed by teacher's explanation, not always clearly translate to the student the dynamics to beadopted for the implementation of the proposed practice. One of these cases is related to the study of the effect of pH on enzyme activity. For better help the understanding of the technical procedure, a hypermedia was built based on a protocol adopted at the Department of Biochemistry, UFSC. The hypermedia shows how theeffect of variations in pH can be observed  in vitro. Taking as example salivary amylase and the consumption of starch (substrate by means of iodine staining, a set of pH buffers was tested to identify the best pH for this enzyme  activity. This hypermedia as introductory tool for such practice was tested on aNutrition course classroom. Students agree that the hypermedia provided a better understanding of the proposed activities. Teachers also notice a smallerreagents consumption and reduction of the time spent by the students in the achievement of the experiment.

  2. CYP17A1: a biochemistry, chemistry, and clinical review.

    Science.gov (United States)

    Porubek, David

    2013-01-01

    Cytochrome P450 17A1 (CYP17A1; also P450c17and P450sccII) is a critically important enzyme in humans that catalyzes the formation of all endogenous androgens. It is an atypical cytochrome P450 enzyme in that it catalyzes two distinct types of substrate oxidation. Through its hydroxylase activity, it catalyzes the 17α-hydroxylation of pregnenolone to 17α-OH pregnenolone. Subsequently, through its C17,20lyase activity, it can further convert 17α-OH pregnenolone to the androgen dehydroepiandrosterone, which is a precursor to androstenedione, testosterone, and dihydrotestosterone. The importance of androgens in diseases such as prostate cancer has been appreciated for decades and the discovery of extra-testicular formation of androgens has helped clarify the pathology of the disease, especially the castrate- resistant disease. Therefore, specific inhibition of CYP17A1 by therapeutic intervention has been an area of considerable effort in several research laboratories. This basic research has led to the discovery of several promising drug candidates followed by the conduct of several clinical trials. Recently, all these efforts have culminated in the first approval by FDA of an inhibitor of CYP17A1 for the treatment of castrate-resistant prostate cancer. Ongoing clinical trials are now evaluating the agent in earlier stages of prostate cancer and even rare forms of androgen-dependent breast cancer. Accordingly, this review focuses on the biochemistry, chemistry, and clinical inhibitors of CYP17A1.

  3. Virtual Practice of Biochemistry: Ascendant Chromatography on Paper of Amino acids.

    Directory of Open Access Journals (Sweden)

    G. Zanatta

    2010-05-01

    Full Text Available Chromatography is a method used for analyses of mixture components based on different criteria: adsorbility, solubility, molecular mass, ionic charge and affinity. It is an important subject of Biochemistry. This work reports the development and the validation of a software that simulated a laboratory activity named Ascendant Chromatography on Paper of Aminoacids. The organization and the multimedia material collection were done during the 2008/2 semester. The most representative images were inserted into the learning object. The tool used for the software development was Adobe® Flash® CS3. The first application of this object was in Biochemistry I (Pharmacy-UFRGS in 2009/1. Using this experience, a new version was developed which was tested by the students of the same subject in 2009/2. After a 50-minute class (theoretical-practical, the students of both semesters were divided in two groups. Group I answered a questionnaire about chromatography basic concepts and after used the software. Group II was submitted to an inverse protocol. The groups also evaluated the technical aspects of the software animation/simulation and the activity carried out. Associating both applications results (2009/1 and 2009/2, the present learning object can be valid as a support for practical teaching of basic biochemistry.

  4. Biochemistry Teaching in the Undergraduate Medical Course at Universidade Federalde Viçosa

    Directory of Open Access Journals (Sweden)

    L. Moreira Lima

    2011-04-01

    Full Text Available Background:The current competencies and abilities required for the training of medical professionals, recommended by the National Curriculum Guidelines for Undergraduate Medicine (DCN have initiated discussions about the teaching of biochemistry in medical schools. Objective: This study aimed to describe the rationale of education in the Clinical Laboratory Applieddisciplines – (LAC I, II, III, IV, V, VI, VII and VIII – which will be taught from first to eighth period of medical school at UFV. Methods:The disciplines weredesigned based on the competencies required for graduates of the course with 30 class hours each, always followingthe period of the study unit, integrated with other disciplines. Results:In the disciplines LACI and LAC II, taught in 2010, thestudents learned basic principles of biosafety, collection and storage of biological samples, validation of diagnostic tests, biochemical tests of cerebrospinal fluid, coagulation and renal function. They learned in the context of solving clinical cases presented and discussed in an integrated manner by a physician and a clinical biochemistry professor. Conclusion:According to the DCN, it is essential to facilitate the construction of knowledge in Biochemistry of themedical student, making the run for making clinical decisions based on scientific evidence.

  5. Haematology and Clinical Biochemistry Findings Associated with Equine Diseases - a Review

    Directory of Open Access Journals (Sweden)

    Onyinyechukwu A. AGINA

    2017-03-01

    Full Text Available The course and outcome of a disease process is dependent upon factors such as the disease-causing agent and its cell tropism, defense mechanisms of the host, genetic resistance of the species or breed affected, as well as the age, nutritional status and hormonal levels of the affected animal. When haematology, clinical biochemistry and cytology test results are combined with other laboratory procedures, complete physical examination and also with the history of the patient, a veterinarian is well armed to arrive at a definitive diagnosis, make a certain prognosis (good, poor or guarded and can also make a concluding statement on the efficacy of the instituted therapy. In clinical biochemistry, demonstration of specific enzyme activity and concentration of analytes in serum/plasma facilitates the disease diagnosis. Also, evaluation of haematology, clinical biochemistry and diagnostic cytology tests can help establish the presence or absence of diseases of internal organs, and by serial performance of these tests, may help to determine whether a disease process remains static, progressive or regressive. This review therefore provides the haematological, serum biochemical and cytological characteristics of diseases caused by the main bacteria, viruses, fungi, protozoa, helminths, arthropods, nutritional deficiencies, endocrine disturbances, neoplasm, allergy, toxins (phytoxins and zootoxins and inorganic poisons in horses.

  6. LLUSTRATION OF AMINO ACIDS REACTIONS AND PROTEINS CHARACTERIZATION FOR EXPERIMENTAL BIOCHEMISTRY CLASSES

    Directory of Open Access Journals (Sweden)

    I. Parreira

    2008-05-01

    Full Text Available New teaching methodologies have been developed to facilitate the learning of biochemistry concepts. A new  approach to Biochemistry  teaching  has become more frequent,  one that does not  require reagents but use photos, videos, softwares etc. Experimental Biochemistry classes, i.e. covering characterization of amino acids and proteins,  might be more productive with the use of complementary didactic material.  Furthermore,  if experiments cannot be implemented, classes may  be well illustrated with complementary didactic material covering from the simplest to the most  complex experiments.  In order to  aid Biochemistry classes without practical experiments, some tests and reactions were documented in our laboratory through digital photos, for  instance: (1 the biuret reaction wherein the blue reagent turns violet in the presence of proteins and changes to pink when combined with short-chain polypeptides; (2 the ninhydrin test used in amino acid analysis of proteins: most of the amino acids are hydrolyzed and react with ninhydrin; when reacting with these free amines, a deep blue or purple color appears; (3 methods for detecting proteins wherein spectrophotometry is used, that deals with the relationship between absorbance, concentration and path length, which constitute the Beer-Lambert Law. A didactic material constituted by texts, schemes and illustrated by photos has been created for each class topic. This material can be used either as a teacher script or in a presentation form to illustrate classes without experimental activities. Financial Support: Pro-Reitoria Graduação-USP, CNPq.

  7. The semiosis of students’ conceptual understanding of biochemistry

    DEFF Research Database (Denmark)

    Musaeus, Peter; Mathiesen, Søren Læssøe; Dahl, Mads Ronald

    2013-01-01

    teaching including biochemistry teaching (Loertscher, 2011). The study seeks an answer to the problem of emergence in science students acquisition of concepts, not only how students form new sign hierarchies over time, but how say easy concepts suddenly change into a hard concepts(or vice versa) as new...... scales) of biochemical concepts were collected every week during a university semester. Archival data (e.g. biochemistry textbooks), diaries and a qualitative interview were collected with a biochemistry teacher. Methodological challenges are identified in studying conceptual change and the applicability...

  8. Idaho National Engineering Laboratory Conceptual Site Treatment Plan. Sections 1 through 8, Tables 2-1 through 6-1, Figures 1 and 2

    Energy Technology Data Exchange (ETDEWEB)

    Eaton, D.

    1993-10-01

    The US Department of Energy (DOE) is required by Section 3021(b) of the Resource Conservation and Recovery Act (RCRA), as amended by the Federal Facility Compliance Act (FFCAct), to prepare plans describing the development of treatment capacities and technologies for treating mixed waste. The FFCAct requires site treatment plans (STPs or plans) to be developed for each site at which DOE generates or stores mixed waste and submitted to the host state or the US Environmental Protection Agency (EPA) for either approval, approval with modification, or disapproval. The Idaho National Engineering Laboratory (INEL) Conceptual Site Treatment Plan (CSTP) is the preliminary version of the plan required by the FFCAct and is being provided to the State of Idaho, the EPA, and others for review. A list of the other DOE sites preparing CSTPs is included in Appendix A of this document. In addition to aiding the INEL in formulating its Final Proposed STP, this CSTP will also provide information to other DOE sites for use in identifying common technology needs and potential options for treating their wastes. The INEL CSTP is also intended to be used in conjunction with CSTPs from other sites as a basis for nationwide discussions among state regulators, the EPA, and other interested parties on treatment strategies and options, and on technical and equity issues associated with DOE`s mixed waste.

  9. Online Communication Tools in Biochemistry Teaching

    Directory of Open Access Journals (Sweden)

    A. O. Ferreira

    2012-05-01

    Full Text Available The  online  communication  tools  enable  new  ways  of  learning, especially  the  forums  in the context of online courses, and the understanding of interactions and collaborations in the  forums  can  improve  them.  The  study  aimed  to  analyze  the  online relationships,  as well  as  obtaining  evidence  of  the  use of  other  learning  tools in  a  biochemistry  subject, focusing on how students use the tool forum and its contribution to learning. The study was  carried  out  from  data  pre  and  post  course  questionnaires  as  well  as  log  of environment  access  and  discussion  forum.  The  forums  have  been  restructured  and systematized  for  analysis  and  creating  discursive  flows  between  statements.  The questionnaires showed the central role of forum and wiki for learning,  the importance of interactions, which was highlighted by the forum analysis. The results indicate that one of the ways to improve online biochemistry teaching is to stimulate interactive activities, participatory  moderation  and  pedagogical  support  by  tutors  and  mentors,  also encouraging  and  creating  strategies  to  collaboration  of  students  to  solve problems  and to collaborative knowledge construction.

  10. Costing clinical biochemistry services as part of an operational management budgeting system.

    Science.gov (United States)

    Tarbit, I F

    1986-08-01

    The process of costing clinical biochemistry tests as a component of the commissioning of a unit management budgeting system based on an International Computers Limited (ICL) minicomputer system was examined. Methods of apportioning consumable and labour costs under direct and indirect cost headings and as test and request charges were investigated, and in this currently operational system it was found that 38% of consumable costs and 57% of labour costs were not a direct component of the routine analysis function. Means of assigning test costs to a given request source and the incorporation of such charges into clinical budget statements were looked at. A reduction in laboratory workload did not produce a comparable reduction in laboratory costs. For a theoretical reduction in workload of 20% only a 3.8% laboratory saving in recoverable costs could be expected.

  11. Can biochemistry drive drug discovery beyond simple potency measurements?

    Science.gov (United States)

    Chène, Patrick

    2012-04-01

    Among the fields of expertise required to develop drugs successfully, biochemistry holds a key position in drug discovery at the interface between chemistry, structural biology and cell biology. However, taking the example of protein kinases, it appears that biochemical assays are mostly used in the pharmaceutical industry to measure compound potency and/or selectivity. This limited use of biochemistry is surprising, given that detailed biochemical analyses are commonly used in academia to unravel molecular recognition processes. In this article, I show that biochemistry can provide invaluable information on the dynamics and energetics of compound-target interactions that cannot be obtained on the basis of potency measurements and structural data. Therefore, an extensive use of biochemistry in drug discovery could facilitate the identification and/or development of new drugs.

  12. International Environmental Law and Biochemistry: An Innovative Teaching Opportunity.

    Science.gov (United States)

    Candlish, John

    1998-01-01

    Explores the ties between international environmental law and biochemistry with respect to genetically modified organisms, biodiversity, marine pollution, cancer biology, and pesticide contamination of food. Contains 30 references. (DDR)

  13. Blood type biochemistry and human disease.

    Science.gov (United States)

    Ewald, D Rose; Sumner, Susan C J

    2016-11-01

    Associations between blood type and disease have been studied since the early 1900s when researchers determined that antibodies and antigens are inherited. In the 1950s, the chemical identification of the carbohydrate structure of surface antigens led to the understanding of biosynthetic pathways. The blood type is defined by oligosaccharide structures, which are specific to the antigens, thus, blood group antigens are secondary gene products, while the primary gene products are various glycosyltransferase enzymes that attach the sugar molecules to the oligosaccharide chain. Blood group antigens are found on red blood cells, platelets, leukocytes, plasma proteins, certain tissues, and various cell surface enzymes, and also exist in soluble form in body secretions such as breast milk, seminal fluid, saliva, sweat, gastric secretions, urine, and amniotic fluid. Recent advances in technology, biochemistry, and genetics have clarified the functional classifications of human blood group antigens, the structure of the A, B, H, and Lewis determinants and the enzymes that produce them, and the association of blood group antigens with disease risks. Further research to identify differences in the biochemical composition of blood group antigens, and the relationship to risks for disease, can be important for the identification of targets for the development of nutritional intervention strategies, or the identification of druggable targets. WIREs Syst Biol Med 2016, 8:517-535. doi: 10.1002/wsbm.1355 For further resources related to this article, please visit the WIREs website.

  14. Impact on human resources: Core Laboratory versus laboratory information system versus modular robotics.

    Science.gov (United States)

    Dadoun, R

    1998-01-01

    Technological advances in laboratory systems have had a great impact on human resources. Surviving the changes requires an in-depth understanding of the technology to implement the appropriate operational model. St. Mary's is a 414-bed, acute care hospital. For 18 months, the laboratories went through the process of moving from a noncomputerized traditional model laboratory (i.e., by discipline) to a fully computerized Core Lab. The Core Lab concept fully integrates biochemistry, hematology, blood bank, and microbiology into two sections (not physically separated): tests processed by automation and tests processed manually. This approach led to a 15% reduction in staff while the volume doubled. The transitions occurred sequentially: from traditional laboratory to Core Lab (noncomputerized), from manual Core Lab to fully computerized Core Lab, and ultimately from a simulation of manual preanalytical phase to automated preanalytical phase (modular robotics). The findings show that Core Lab and computerization have almost the same impact on human resources, 35% and 30% respectively, and modular robotics the least impact with 17%.

  15. Writing throughout the biochemistry curriculum: Synergistic inquiry-based writing projects for biochemistry students.

    Science.gov (United States)

    Mertz, Pamela; Streu, Craig

    2015-01-01

    This article describes a synergistic two-semester writing sequence for biochemistry courses. In the first semester, students select a putative protein and are tasked with researching their protein largely through bioinformatics resources. In the second semester, students develop original ideas and present them in the form of a research grant proposal. Both projects involve multiple drafts and peer review. The complementarity of the projects increases student exposure to bioinformatics and literature resources, fosters higher-order thinking skills, and develops teamwork and communication skills. Student feedback and responses on perception surveys demonstrated that the students viewed both projects as favorable learning experiences.

  16. The Biochemistry Over 20 Years In The High School Textbooks

    Directory of Open Access Journals (Sweden)

    C. E.S. Rocha

    2014-08-01

    Full Text Available   The Biochemistry Over 20 Years In The High School Textbooks   Rocha, C. E. S.1; Büttenbender, M. D.1; Denardin, E.L.G.2, Roehrs, R.1,2 1Grupo Interdisciplinar de Pesquisa em Práticas de Ensino, UNIPAMPA, RS. 2Laboratório de Estudos Físico Químicos e Produtos Naturais, UNIPAMPA, RS.   INTRODUCTION: Many teachers make use of textbook to lead content in the classroom. The chemistry books introduce concepts that should relate biochemistry to students in schools. It is important that this first contact turns out into an encouraging experience for the students, because once it worked as expected it arouses interest and makes the students see themselves curious to delve into the subject. The research aims to evaluate the presence of related concepts in biochemistry textbooks in chemistry in high school, over 20 years. MATERIAL AND METHODS: In order to perform this study, we analyzed the following content related to biochemistry: proteins, carbohydrates, lipids and nucleic acids in the books "Chemistry - Structure of Matter and Organic Chemistry" of the year 1993 and the book "Chemistry in approach to daily life" of the year 2012 with the purpose of verifying the changes in the content of biochemistry in the last 20 years. RESULTS AND DISCUSSION: In the 90s, as used in the book, concepts and explanations are introduced in a very objective approach, making a total of 22 pages. The current largest is 23 pages with experiments and curiosities. Through analysis we found that current textbooks present the same issues related to biochemistry, however, a greater amount of data, bringing students to more examples and applications in everyday life. Today we see that the contents and processes are most exploited and that there is a concern on the importance of the study of issues that relate to biochemistry. CONCLUSIONS: The study of the biochemistry textbooks has been more attractive in recent years, contextualizing content with the daily life of

  17. The Biochemistry Show: a new and fun tool for learning

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    A.H Ono

    2006-07-01

    Full Text Available The traditional methods to teach biochemistry in most universities are based on the memorization of chemical structures,  biochemical  pathways  and  reagent  names,  which  is  many  times  dismotivating  for  the  students.  We presently describe an innovative, interactive and alternative method for teaching biochemistry to medical and nutrition undergraduate students, called the Biochemistry Show (BioBio Show.The Biobio show is based on active participation of the students. They are divided in groups and the groups face each other. One group faces another one group at a time, in a game based on true or false questions that involve subjects of applied biochemistry (exercise, obesity, diabetes, cholesterol, free radicals, among others. The questions of the Show are previously elaborated by senior students. The Biobio Show has four phases, the first one is a selection exam, and from the second to the fourth phase, eliminatory confrontations happen. On a confrontation, the first group must select a certain quantity of questions for the opponent to answer.  The group who choses the questions must know how to answer and justify the selected questions. This procedure is repeated on all phases of the show. On the last phase, the questions used are taken from an exam previously performed by the students: either the 9-hour biochemistry exam (Sé et al. A 9-hour biochemistry exam. An iron man competition or a good way of evaluating undergraduate students? SBBq 2005, abstract K-6 or the True-or-False exam (TFE (Sé et al. Are tutor-students capable of writing good biochemistry exams? SBBq 2004, abstract K-18. The winner group receives an extra 0,5 point on the final grade. Over 70% of the students informed on a questionnaire that the Biobio Show is a valuable tool for learning biochemistry.    That is a new way to enrich the discussion of biochemistry in the classroom without the students getting bored. Moreover, learning

  18. Recent perspectives into biochemistry of decavanadate

    Institute of Scientific and Technical Information of China (English)

    Manuel; Aureliano

    2011-01-01

    The number of papers about decavanadate has doubled in the past decade. In the present review, new insights into decavanadate biochemistry, cell biology, and antidiabetic and antitumor activities are described. Decameric vanadate species (V10) clearly differs from monomeric vanadate (V1), and affects differently calcium pumps, and structure and function of myosin and actin. Only decavanadate inhibits calcium accumulation by calcium pump ATPase, and strongly inhibits actomyosin ATPase activity (IC50 = 1.4 μmol/L, V10), whereas no such ef- fects are detected with V1 up to 150 μmol/L; prevents actin polymerization (IC50 of 68 μmol/L, whereas no effects detected with up to 2 mmol/L V1); and interacts with actin in a way that induces cysteine oxidation and vanadate reduction to vanadyl. Moreover, in vivo decavanadate toxicity studies have revealed that acute exposure to polyoxovanadate induces different changes in antioxidant enzymes and oxidative stress parameters, in comparison with vanadate. In vitro studies have clearly demonstrated that mitochondrial oxygen consumption is strongly affected by decavanadate (IC50, 0.1 μmol/L); perhaps the most relevant biological effect. Finally, decavanadate (100 μmol/L) increases rat adipocyte glucose accumulation more potently than several vanadium complexes. Preliminary studies sug- gest that decavanadate does not have similar effects in human adipocytes. Although decavanadate can be a useful biochemical tool, further studies must be carried out before it can be conf irmed that decavanadate and its complexes can be used as anticancer or antidiabetic agents.

  19. Recent perspectives into biochemistry of decavanadate.

    Science.gov (United States)

    Aureliano, Manuel

    2011-10-26

    The number of papers about decavanadate has doubled in the past decade. In the present review, new insights into decavanadate biochemistry, cell biology, and antidiabetic and antitumor activities are described. Decameric vanadate species (V(10)) clearly differs from monomeric vanadate (V(1)), and affects differently calcium pumps, and structure and function of myosin and actin. Only decavanadate inhibits calcium accumulation by calcium pump ATPase, and strongly inhibits actomyosin ATPase activity (IC(50) = 1.4 μmol/L, V(10)), whereas no such effects are detected with V(1) up to 150 μmol/L; prevents actin polymerization (IC(50) of 68 μmol/L, whereas no effects detected with up to 2 mmol/L V(1)); and interacts with actin in a way that induces cysteine oxidation and vanadate reduction to vanadyl. Moreover, in vivo decavanadate toxicity studies have revealed that acute exposure to polyoxovanadate induces different changes in antioxidant enzymes and oxidative stress parameters, in comparison with vanadate. In vitro studies have clearly demonstrated that mitochondrial oxygen consumption is strongly affected by decavanadate (IC(50), 0.1 μmol/L); perhaps the most relevant biological effect. Finally, decavanadate (100 μmol/L) increases rat adipocyte glucose accumulation more potently than several vanadium complexes. Preliminary studies suggest that decavanadate does not have similar effects in human adipocytes. Although decavanadate can be a useful biochemical tool, further studies must be carried out before it can be confirmed that decavanadate and its complexes can be used as anticancer or antidiabetic agents.

  20. Collective Construction of Knowledge in Clinical Biochemistry

    Directory of Open Access Journals (Sweden)

    S.B. Barreto

    2010-05-01

    Full Text Available The collective construction of knowledge occurs by the convergence of ideas and semantic. This paper was made for a graduation discipline, in 2009-2, with 240students who were separated into 4 groups: morning period (M1,M2 and night period (N1,N2. This study aims the collective construction of a abstract-manual of clinical biochemistry tests, due the difficulty in comprehension of certain concepts by the students; it intends to help them in the process of knowledge acquirement. The constructivist approach was adopted and the matters of the discipline were available in a “Student Group e-mail account”, a functional communication tool. The instructions were reachable on the web. M1,M2 and N1 made one part of the study at the first period. N2 did not conclude the study at the same time period of the other groups; therefore they received a new responsibility: they were supposed to conclude and correct the manual and its application which included 90 different kinds of labor exams. A textbook has been defined containing illustrative pictures of blood collection and biosecurity. Three banners were exposed inside the hall of the institution. Collective work is important for the effective arrangement in health area. In the process of teaching/learning, the teacher must proceed on practices and methodologies aiming the development of the student competences and skills which represent its professional identity.

  1. Using 3DClass To Flip Biochemistry Classroom

    Directory of Open Access Journals (Sweden)

    T. Silva

    2014-08-01

    Full Text Available The flipped classroom inverts traditional teaching methods, in order to have studentsprepared for topics and techniques covered in the following meeting. This approach wasadopted in a biochemistry course taught to biology freshmen students at the University ofCampinas, using a Virtual Learning Environment called 3DClass. Before each classroomsession, a quiz was delivered covering the following topic and students were allowed totake quizzes as many times as they wanted. This approach was utilized in order to betterprepare students in classes and to perform lab experiments. Every student attempt wasrecorded in a database. Before each classroom session, the instructors were provided witha summary of the class answers, highlighting questions where students had more difficultyand the ones that scored higher. This kind of information was helpful to design activities tocover the topics where students had more difficulties. Based on the 3DClass records thestudents behaviors were mapped, such as students taking the quizzes seriously, studentsguessing, students answering a quiz until scoring 100%, students that continue answeringafter scoring 100% in order to increase their grades, students that never score 100%.However, the most relevant information 3DClass brought us was the possibility to identifystudent’s confidence in their answers, which could be observed by the analysis of theirattempts for each question. If they had made different choices each try, it would haveindicated a low confidence level, while always providing the same answer indicated ahigher confidence level, even whilst picking incorrect answers. This experiment haverevealed that students coming to the classroom better prepared reflected positively on thedeveloped activities, but the number of students taking the quizzes seriously were not asgreat as we had expected, indicating that more actions should be taken to improvestudents engagement with these activities.

  2. Geobiochemistry: Placing Biochemistry in Its Geochemical Context

    Science.gov (United States)

    Shock, E.; Boyer, G. M.; Canovas, P. A., III; Prasad, A.; Dick, J. M.

    2014-12-01

    Goals of geobiochemistry include simultaneously evaluating the relative stabilities of microbial cells and minerals, and predicting how the composition of biomolecules can change in response to the progress of geochemical reactions. Recent developments in theoretical geochemistry make it possible to predict standard thermodynamic properties of proteins, nucleotides, lipids, and many metabolites including the constituents of the citric acid cycle, at all temperatures and pressures where life is known to occur, and beyond. Combining these predictions with constraints from geochemical data makes it possible to assess the relative stabilities of biomolecules. Resulting independent predictions of the environmental occurrence of homologous proteins and lipid side-chains can be compared with observations from metagenomic and metalipidomic data to quantify geochemical driving forces that shape the composition of biomolecules. In addition, the energetic costs of generating biomolecules from within a diverse range of habitable environments can be evaluated in terms of prevailing geochemical variables. Comparisons of geochemical bioenergetic calculations across habitats leads to the generalization that the availability of H2 determines the cost of autotrophic biosynthesis relative to the aquatic environment external to microbial cells, and that pH, temperature, pressure, and availability of C, N, P, and S are typically secondary. Increasingly reduced conditions, which are determined by reactions of water with mineral surfaces and mineral assemblages, allow many biosynthetic reactions to shift from costing energy to releasing energy. Protein and lipid synthesis, as well as the reverse citric acid cycle, become energy-releasing processes under these conditions. The resulting energy balances that determine habitability contrast dramatically with assumptions derived from oxic surface conditions, such as those where human biochemistry operates.

  3. BIOCHEMISTRY OF SOME PERSPECTIVE TABLE GRAPES

    Directory of Open Access Journals (Sweden)

    Burlakov M. M.

    2015-03-01

    Full Text Available The article contains results of the study of biochemistry of some perspective table grape varieties: Aniuta, Vodograi, Nizina and Jubilee Novocherkasska, yield in 2013 from the Central zone of Krasnodar region. At the time of redy yield the combination of sweetness and acidity of fruits were favorable. The ratio of fructose to glucose was 1.69; 1.36; 2.00; 1.13, respectively, in grapes Aniuta, Vodograi, Nisina, Jubilee Novocherkasska. According to the percentage of fructose in fruit juice varieties Aniuta, Vodograi and Jubilee Novocherkasska close to each other (50.93; 56.14; 51.23%. According to the percentage of glucose similar varieties Aniuta and Nizina (33 - 34.27%, Vodograi and Jubilee Novocherkasska (41.16 - 45.22%. Sort Jubilee Novocherkasska, had content in berries close fructose and glucose. Most of fructose (66.02% in berries variety Nizina. In berries studied cultivars organic acid content was: wine 3128 - 3861, malic 982 - 3753, citric 109 - 317, amber 3 - 115 mg / dm3. The highest concentrations of potassium and sodium cations are presented in berries varieties Aniuta: potassium - 1219 sodium - 35.13 mg / dm3 and cations magnesium and calcium in berries Jubilee Novocherkasska 46.75 and 73.64 mg / dm3. The lowest concentration of potassium and sodium cations observed in grape Vodograi, magnesium - from grape Aniuta, calcium - at grape Nizina. We studied grape bunches are large, not dense, beautiful, berries are large, harmonious taste. Tasting score fresh grapes varieties Aniuta, Vodograi, Nizina, Jubilee Novocherkasska 7.6; 7.4; 7.8; 7.8 points respectively

  4. Population-based biochemistry, immunologic and hematological reference values for adolescents and young adults in a rural population in Western Kenya.

    Directory of Open Access Journals (Sweden)

    Clement Zeh

    Full Text Available BACKGROUND: There is need for locally-derived age-specific clinical laboratory reference ranges of healthy Africans in sub-Saharan Africa. Reference values from North American and European populations are being used for African subjects despite previous studies showing significant differences. Our aim was to establish clinical laboratory reference values for African adolescents and young adults that can be used in clinical trials and for patient management. METHODS AND FINDINGS: A panel of 298, HIV-seronegative individuals aged 13-34 years was randomly selected from participants in two population-based cross-sectional surveys assessing HIV prevalence and other sexually transmitted infections in western Kenya. The adolescent (<18 years-to-adults (≥ 18 years ratio and the male-to-female ratio was 1∶1. Median and 95% reference ranges were calculated for immunohematological and biochemistry values. Compared with U.S-derived reference ranges, we detected lower hemoglobin (HB, hematocrit (HCT, red blood cells (RBC, mean corpuscular volume (MCV, neutrophil, glucose, and blood urea nitrogen values but elevated eosinophil and total bilirubin values. Significant gender variation was observed in hematological parameters in addition to T-bilirubin and creatinine indices in all age groups, AST in the younger and neutrophil, platelet and CD4 indices among the older age group. Age variation was also observed, mainly in hematological parameters among males. Applying U.S. NIH Division of AIDS (DAIDS toxicity grading to our results, 40% of otherwise healthy study participants were classified as having an abnormal laboratory parameter (grade 1-4 which would exclude them from participating in clinical trials. CONCLUSION: Hematological and biochemistry reference values from African population differ from those derived from a North American population, showing the need to develop region-specific reference values. Our data also show variations in hematological

  5. FACEBOOK AS A MEDIATION TOOL IN BIOCHEMISTRY DISCIPLINE

    Directory of Open Access Journals (Sweden)

    P. X. Gomes

    2015-08-01

    Full Text Available Introduction: The current students generation are daily connected to the Internet, wich encourages the use of mobile tools in education. Many of the students of Biochemistry feel apprehensive about the discipline and the use of facebook may contribute, among other factors, motivating them. Objectives: It was analyzed the use of facebook as a mediator and motivator in the discipline of Biochemistry, basing on socioconstrutivist interventions. Material and methods: This work was developed in the action-research perspective, using the quali-quantitative method. An investigative questionnaire was used, using Likert scale and open questions, to investigate the facebook use, as well as the preferences of students, focusing on Biochemistry group in the Biomedicine course.  The posts were analyzed identifying: frequency of the interaction`s types (post, comment, likes;  interaction's categories (question, answer, motivational; and the content itself of the post. Results: It was highlighted students' interest to search materials, answering questions, and especially seeking information about the discipline. It was emphasized that the group was motivating for learning Biochemistry, encouragement the group to study, with quick and easy access to the professor by chat. Conclusions: The results indicate a preference for students at facebook, with a great motivational potential, is at easy access to colleagues, professor and monitor, or even the ease of obtaining the materials and ask questions in real time, indicating that this tool as a possible way, still little explored, to enhance the teaching of Biochemistry.

  6. A comprehensive training approach for biomedical engineers in biochemistry and in vitro diagnostics technology.

    Science.gov (United States)

    Spyropoulos, Basile; Tzavaras, Aris

    2007-01-01

    The purpose of this paper is to review 20 years (1987-2007) of experience in training young Biomedical Engineers in Biochemistry and in vitro Diagnostics (IVD) Technology. This encountering has resulted in the gradual formation of a comprehensive training package that includes lectures and laboratory practicals, supported by both, traditional and on-line digital means, such as lecture-notes, slides, videos, demos and equipment simulations. Further, this course is maintained up to date by several research and development activities that offer partially feed back to the course and enrich its contents with custom developed devices, methods and application software. In this paper are presented, first, the structure and the components of this course, and second, the most important custom developed novelties, which have been integrated in the IVD Technology laboratory-practicals.

  7. Haematology and biochemistry values of captive sand cats (Felis margarita) in Al Ain Wildlife Park and Resort, United Arab Emirates

    Institute of Scientific and Technical Information of China (English)

    Stephen Chege; Arshad Toosy; Judith Howlett; Ahmed Saker; John Kagira

    2013-01-01

    Objective: To investigate the haematology and biochemistry values of apparently healthy captive sand cats kept in Al Ain Wildlife Park and Resort, United Arab Emirates, with a view to establishing baseline values.Methods:Blood was collected from the femoral vein using aseptic techniques, kept in a cool box and sent to laboratory for analysis. The blood was analysed for haematological and biochemical values using veterinary hematology and chemistry analysers (ABX ABC Vet, Horiba ABX SAS Montpellier, France). Results: Haematological values were within the normal ranges recorded in domestic cats and there was no statistical difference between values found in males and females. Aspartate aminotransferase values were higher (P0.05) between males and females values.Conclusions:Our results present reference ranges for haematology and biochemistry parameters in captive sand cats. These values will be important for diagnosis of various diseases and monitoring of treatments.

  8. My Lifelong Passion for Biochemistry and Anaerobic Microorganisms.

    Science.gov (United States)

    Thauer, Rudolf Kurt

    2015-01-01

    Early parental influence led me first to medical school, but after developing a passion for biochemistry and sensing the need for a deeper foundation, I changed to chemistry. During breaks between semesters, I worked in various biochemistry labs to acquire a feeling for the different areas of investigation. The scientific puzzle that fascinated me most was the metabolism of the anaerobic bacterium Clostridium kluyveri, which I took on in 1965 in Karl Decker's lab in Freiburg, Germany. I quickly realized that little was known about the biochemistry of strict anaerobes such as clostridia, methanogens, acetogens, and sulfate-reducing bacteria and that these were ideal model organisms to study fundamental questions of energy conservation, CO2 fixation, and the evolution of metabolic pathways. My passion for anaerobes was born then and is unabated even after 50 years of study.

  9. The use of multiple tools for teaching medical biochemistry.

    Science.gov (United States)

    Sé, Alexandre B; Passos, Renato M; Ono, André H; Hermes-Lima, Marcelo

    2008-03-01

    In this work, we describe the use of several strategies employing the philosophies of active learning and problem-based learning (PBL) that may be used to improve the teaching of metabolic biochemistry to medical and nutritional undergraduate students. The main activities are as follows: 1) a seminar/poster system in a mini-congress format (using topics of applied biochemistry); 2) a true/false applied biochemistry exam (written by peer tutors); 3) a 9-h exam on metabolism (based in real publications); 4) the Advanced Biochemistry course (directed to peer tutors, where students learn how to read and criticize real medical papers); 5) experiments about nutrition and metabolism, using students as volunteers, and about free radicals (real science for students); 6) the BioBio blog (taking advantage of the "web age," this enhances out of class exchanges of information between the professor, students, and peer tutors); 7) student lectures on public health issues and metabolic disorders directed to the community and lay people; and 8) the BioBio quiz show. The main objective of these activities is to provide students with a more practical and interesting approach to biochemistry, such as the application of theoretical knowledge to real situations (diseases, experiments, media information, and scientific discoveries). In addition, we emphasize the importance of peer tutor activities for optimized learning of both students and peer tutors, the importance of a closer interaction between students and teaching staff, and the necessity to initiate students precociously in two broad fields of medical activity: "real" basic science and contact with the public (also helping students--future doctors and nutritionists--to be able to communicate with lay people). Most activities were evaluated by the students through written questionnaires and informal conversations, along various semesters, indicating good acceptance and approval of these methods. Good student scores in the

  10. Game Development as Didactic Strategy for Biochemistry Teaching

    OpenAIRE

    G. G. Hornink

    2010-01-01

    It is well known that students and teachers have difficulties in learning and teaching Biochemistry due to its abstract and interconnected contents. This work proposes a didactic strategy in order to facilitate teaching and learning process in Biochemistry. The strategy was implemented with biological science undergraduate students. At first, the students were divided into groups with a specific topic to develop a game. During the semester, problem based learning cases, online activities like...

  11. Teaching structure: student use of software tools for understanding macromolecular structure in an undergraduate biochemistry course.

    Science.gov (United States)

    Jaswal, Sheila S; O'Hara, Patricia B; Williamson, Patrick L; Springer, Amy L

    2013-01-01

    Because understanding the structure of biological macromolecules is critical to understanding their function, students of biochemistry should become familiar not only with viewing, but also with generating and manipulating structural representations. We report a strategy from a one-semester undergraduate biochemistry course to integrate use of structural representation tools into both laboratory and homework activities. First, early in the course we introduce the use of readily available open-source software for visualizing protein structure, coincident with modules on amino acid and peptide bond properties. Second, we use these same software tools in lectures and incorporate images and other structure representations in homework tasks. Third, we require a capstone project in which teams of students examine a protein-nucleic acid complex and then use the software tools to illustrate for their classmates the salient features of the structure, relating how the structure helps explain biological function. To ensure engagement with a range of software and database features, we generated a detailed template file that can be used to explore any structure, and that guides students through specific applications of many of the software tools. In presentations, students demonstrate that they are successfully interpreting structural information, and using representations to illustrate particular points relevant to function. Thus, over the semester students integrate information about structural features of biological macromolecules into the larger discussion of the chemical basis of function. Together these assignments provide an accessible introduction to structural representation tools, allowing students to add these methods to their biochemical toolboxes early in their scientific development.

  12. Lecture-Free Biochemistry: A Process Oriented Guided Inquiry Approach

    Science.gov (United States)

    Minderhout, Vicky; Loertscher, Jennifer

    2007-01-01

    Biochemistry courses at Seattle University have been taught exclusively using process oriented guided inquiry learning (POGIL) without any traditional lecture component since 1997. In these courses, students participate in a structured learning environment, which includes a preparatory assignment, an in-class activity, and a follow-up skill…

  13. Osmotic Stressing, Membrane Leakage, and Fluorescence: An Introductory Biochemistry Demonstration

    Science.gov (United States)

    Seu, Kalani J.

    2015-01-01

    A fluorescence demonstration is described that incorporates several fundamental aspects of an introductory biochemistry course. A variation of a known leakage assay is utilized to prepare vesicles containing a quenched fluorophore. The vesicles are exposed to several osmotic environments ranging from isotonic to hypotonic. The degree of vesicle…

  14. Estimating foliar biochemistry from hyperspectral data in mixed forest canopy

    DEFF Research Database (Denmark)

    Huber Gharib, Silvia; Kneubühler, Mathias; Psomas, Achilleas

    2008-01-01

    data to estimate the foliar concentration of nitrogen, carbon and water in three mixed forest canopies in Switzerland. With multiple linear regression models, continuum-removed and normalized HyMap spectra were related to foliar biochemistry on an individual tree level. The six spectral wavebands used...

  15. An Integrated Strategy for Teaching Biochemistry to Biotechnology Specialty Students

    Science.gov (United States)

    Ouyang, Liming; Ou, Ling; Zhang, Yuanxing

    2007-01-01

    The faculty of biochemistry established an integrated teaching strategy for biotechnology specialty students, by intermeshing the case-study method, web-assistant teaching, and improved lecture format with a brief content and multimedia courseware. Teaching practice showed that the integrated teaching strategy could retain the best features of…

  16. Using Assessment to Improve Learning in the Biochemistry Classroom

    Science.gov (United States)

    Loertscher, Jennifer

    2010-01-01

    In recent years, major drivers of undergraduate science education reform including the National Science Foundation (NSF) and the Howard Hughes Medical Institute (HHMI) have called on college and university instructors to take a more scientific approach to their teaching. Although many biochemistry instructors are gaining confidence in using…

  17. Assessment of Learning Gains in a Flipped Biochemistry Classroom

    Science.gov (United States)

    Ojennus, Deanna Dahlke

    2016-01-01

    The flipped classroom has become an increasingly popular pedagogical approach to teaching and learning. In this study, learning gains were assessed in a flipped biochemistry course and compared to gains in a traditional lecture. Although measured learning gains were not significantly different between the two courses, student perception of…

  18. Uncovering Students' Incorrect Ideas about Foundational Concepts for Biochemistry

    Science.gov (United States)

    Villafane, Sachel M.; Loertscher, Jennifer; Minderhout, Vicky; Lewis, Jennifer E.

    2011-01-01

    This paper presents preliminary data on how an assessment instrument with a unique structure can be used to identify common incorrect ideas from prior coursework at the beginning of a biochemistry course, and to determine whether these ideas have changed by the end of the course. The twenty-one multiple-choice items address seven different…

  19. Biochemistry Instructors' Perceptions of Analogies and Their Classroom Use

    Science.gov (United States)

    Orgill, MaryKay; Bussey, Thomas J.; Bodner, George M.

    2015-01-01

    Biochemistry education relies heavily on students' abilities to conceptualize abstract cellular and molecular processes, mechanisms, and components. From a constructivist standpoint, students build their understandings of these abstract processes by connecting, expanding, or revising their prior conceptions and experiences. As such, biochemistry…

  20. Biochemistry Students' Ideas about Shape and Charge in Enzyme-Substrate Interactions

    Science.gov (United States)

    Linenberger, Kimberly J.; Bretz, Stacey Lowery

    2014-01-01

    Biochemistry is a visual discipline that requires students to develop an understanding of numerous representations. However, there is very little known about what students actually understand about the representations that are used to communicate ideas in biochemistry. This study investigated biochemistry students' understanding of multiple…

  1. DMPD: Type I interferon receptors: biochemistry and biological functions. [Dynamic Macrophage Pathway CSML Database

    Lifescience Database Archive (English)

    Full Text Available 17502368 Type I interferon receptors: biochemistry and biological functions. de Wee...(.html) (.csml) Show Type I interferon receptors: biochemistry and biological functions. PubmedID 17502368 T...itle Type I interferon receptors: biochemistry and biological functions. Authors

  2. Clinical Laboratory Fee Schedule

    Data.gov (United States)

    U.S. Department of Health & Human Services — Outpatient clinical laboratory services are paid based on a fee schedule in accordance with Section 1833(h) of the Social Security Act. The clinical laboratory fee...

  3. RCRA Facility Investigation report for Waste Area Grouping 6 at Oak Ridge National Laboratory, Oak Ridge, Tennessee. Volume 2, Sections 4 through 9: Environmental Restoration Program

    Energy Technology Data Exchange (ETDEWEB)

    1991-09-01

    This report presents compiled information concerning a facility investigation of waste area group 6(WAG-6), of the solid waste management units (SWMU`s) at Oak Ridge National Laboratory (ORNL). The WAG is a shallow ground disposal area for low-level radioactive wastes and chemical wastes. The report contains information on hydrogeological data, contaminant characterization, radionuclide concentrations, risk assessment and baseline human health evaluation including a toxicity assessment, and a baseline environmental evaluation.

  4. RCRA Facility Investigation report for Waste Area Grouping 6 at Oak Ridge National Laboratory, Oak Ridge, Tennessee. Volume 2. Sections 4 through 9

    Energy Technology Data Exchange (ETDEWEB)

    None

    1991-09-01

    This report presents compiled information concerning a facility investigation of waste area group 6(WAG-6), of the solid waste management units (SWMU's) at Oak Ridge National Laboratory (ORNL). The WAG is a shallow ground disposal area for low-level radioactive wastes and chemical wastes. The report contains information on hydrogeological data, contaminant characterization, radionuclide concentrations, risk assessment and baseline human health evaluation including a toxicity assessment, and a baseline environmental evaluation.

  5. Playing with a double-edged sword: Analogies in biochemistry

    Science.gov (United States)

    Orgill, Marykay

    Analogy pervades our everyday reasoning. No situation we encounter is exactly like a situation we have encountered previously, and our ability to learn and survive in the world is based on our ability to find similarities between past and present situations and use the knowledge we have gained from past situations to manage current situations. Analogies can be powerful teaching tools because they can make new material intelligible to students by comparing it to material that is already familiar. It is clear, though, that not all analogies are good and that not all good analogies are useful to all students. In this study, I have used textbook analysis, classroom observations, student interviews and instructor interviews to determine the role that analogies play in biochemistry learning. Analogies are an important teaching technique in biochemistry classes, being used more often in both biochemistry classes and textbooks than they are in high school chemistry classes and textbooks. Most biochemistry students like, pay particular attention to, and remember the analogies their instructors provide; and they use these analogies to understand, visualize, and recall information from class. Even though students like and use analogies, they do not understand what analogies are or the mechanism by which they improve learning. For the students, analogies are simply any teaching technique that eases understanding, visualization, or recall. Instructors, on the other hand, have a good understanding of what analogies are and of how they should be presented in class; but they do not use analogies as effectively as they should. They do not plan, explain or identify the limitations of the analogies they use in class. However, regardless of how effectively instructors present analogies in class, this study indicates that, in general, analogies are useful in promoting understanding, visualization, recall, and motivation in biochemistry students at all levels. They would be even more

  6. High acceptability for cell phone text messages to improve communication of laboratory results with HIV-infected patients in rural Uganda: a cross-sectional survey study

    Directory of Open Access Journals (Sweden)

    Siedner Mark J

    2012-06-01

    Full Text Available Abstract Background Patient-provider communication is a major challenge in resource-limited settings with large catchment areas. Though mobile phone usership increased 20-fold in Africa over the past decade, little is known about acceptability of, perceptions about disclosure and confidentiality, and preferences for cell phone communication of health information in the region. Methods We performed structured interviews of fifty patients at the Immune Suppression Syndrome clinic in Mbarara, Uganda to assess four domains of health-related communication: a cell phone use practices and literacy, b preferences for laboratory results communication, c privacy and confidentiality, and d acceptability of and preferences for text messaging to notify patients of abnormal test results. Results Participants had a median of 38 years, were 56% female, and were residents of a large catchment area throughout southwestern Uganda. All participants expressed interest in a service to receive information about laboratory results by cell phone text message, stating benefits of increased awareness of their health and decreased transportation costs. Ninety percent reported that they would not be concerned for unintended disclosure. A minority additionally expressed concerns about difficulty interpreting messages, discouragement upon learning bad news, and technical issues. Though all respondents expressed interest in password protection of messages, there was also a strong desire for direct messages to limit misinterpretation of information. Conclusions Cell phone text messaging for communication of abnormal laboratory results is highly acceptable in this cohort of HIV-infected patients in rural Uganda. The feasibility of text messaging, including an optimal balance between privacy and comprehension, should be further studied.

  7. THE EVOLUTION OF THE KREBS CYCLE: A PROMISING THEME FOR MEANINGFUL BIOCHEMISTRY LEARNING IN BIOLOGY

    Directory of Open Access Journals (Sweden)

    C. Costa

    2015-08-01

    Full Text Available INTRODUCTION: Evolution has been recognized as a key concept for biologists. In order to motivate biology undergraduates for contents of central energetic metabolism, we addressed the Krebs cycle structure and functions to an evolutionary view. To this end, we created a study guide which contextualizes the emergence of the cyclic pathway, in light of the prokaryotic influence since early Earth anaerobic condition to oxygen rise in atmosphere. OBJECTIVES: The main goal is to highlight the educational potential of the material whose subject is scarcely covered in biochemistry textbooks. MATERIALS AND METHODS: The study guide is composed by three interrelated sections, the problem (Section 1, designed to arouse curiosity, inform and motivate students; an introductory text (Section 2 about life evolution, including early micro-organisms and Krebs cycle emergence, and questions (Section 3 for debate. The activity consisted on a peer discussion session, with instructors tutoring. The questions were designed to foster exchange of ideas in an ever-increasing level of complexity, and cover subjects from early atmospheric conditions to organization of the metabolism along the subsequent geological ages. RESULTS AND DISCUSSION: We noticed that students were engaged and motivated by the task, especially during group discussion. Based on students’ feedbacks and class observations, we learned that the material raised curiosity and stimulated discussion among peers. It brought a historical and purposeful way of dealing with difficult biochemical concepts. CONCLUSIONS: The whole experience suggests that the study guide was a stimulus for broadening comprehension of the Krebs cycle, reinforcing the evolutionary stance as an important theme for biology and biochemistry understanding. On the other hand, we do not underestimate the fact that approaching Krebs cycle from an evolutionary standpoint is a quite complex discussion for the majority of students

  8. Alien Biochemistries and Their Metabolic By-Products. Lessons from Synthetic Biology

    Science.gov (United States)

    Benner, S.

    2014-03-01

    While the metabolisms of terran organisms are accessible for study and their byproducts are, for the most part, well known, the "diversity" of terran biology arises (as far as we know) from a single common ancestor, represents only a small fraction of possible chemical difersity, and may reflect only a fraction of the possible chemical diversity that might support Darwinian evolution [1]. This talk will consider laboratory experiments on origins [2] and synthetic biology [3], asking how they might inform us about alternative biochemistries, and whether we have any chance of observing remotely their by-products, recognizing the uncertanties in both our models for "weird life" and our models of abiotic processes in incompletely defined planetary environments.

  9. Biochemistry on the Media: daily science in audio and video

    Directory of Open Access Journals (Sweden)

    B. P. Melo et al

    2014-08-01

    Full Text Available Biochemistry on the Media: daily science in audio and video Melo,B. P1; Henriques, L. R1; Júnior, H. G2; Galvão, G. R2; Costa, M. M2; Silva, A. S3; Costa, M. P3; Barreto, L. P3; Almeida, A. A3; Fontes, P. P3; Meireles, L. M3; Costa, P. A3; Costa, C. B3; Monteiro, L. M. O3 Konig, I. M3; Dias, B. K. M1; Santos, R. C. V1; Bagno, F. F1; Fernandes, L1; Alves, P. R1; Sales, F. M1; Martins, T. C. N1; Moreira, V. J. V1; Marchiori, J. M1; Medeiros, L.4; Leite, J. P. V5; Moraes, G. H. K6.   1 Members of ETP-Biochemistry UFV; 2 Students of program Jovens Talentos para a Ciência UFV; 3 Graduating Students of ETP; 4 Coordinator in Espaço Ciência UFV; 5 Pharmaceutical, professor at Molecular Biology and Biochemistry Department (BBD UFV, ETP’s tutor; 6 Agronomist, professor at BDD, work’s advisor.   INTRODUCTION: The Educational Tutorial Program in Biochemistry (ETP from UFV have worked in qualification of basic science teachers, offering courses about Biochemistry. In courses, was detected the necessity of a personal material to inspire them. To do it, ETP compiled some media spots in a box and have used it in qualification courses. OBJECTIVES: The objective of this work was construct a part of a permanent material to be used in courses to qualifications high school's teachers and evaluate it. METODOLOGY: Applying questionnaires to high school students, ETP's members had detected that these students don't have a solid idea about how is Biochemistry. Thus, themes about common Biochemistry daily things were elected to be transformed in spots to radio and television. Texts about shampoo composition, vegetable’s darkening, bread’s fermentation, etc, were written and a script done by Journalism’s students of Espaço Ciência(*. Finally, the spots were recorded and vehiculated on universitary channel. In 2013, the spots were compiled in a media box. It has been included in a permanent material used in qualification courses. According to ALBAGLI

  10. Resillient modulus of freez-thaw affected granular soils for pavement design and evaluation. Part 1: Laboratory tests on soils from Winchendon, Massachusetts, test sections

    Science.gov (United States)

    Cole, D.; Bentley, D.; Durell, G.; Johnson, T.

    1986-07-01

    This is the first of a series of four reports about laboratory and field testing of various granular road and airfield subgrades. This report details the acquisition, testing and analysis of six soils from a test site in Winchendon, Massachusetts. Repeated load triaxial tests were done on frozen and thawed soils to characterize the variations in the their resilient properties throughout the seasons. Linear regression yielded empirical equations relating the resilient modulus to applied stress, unfrozen water content (for frozen soils), moisture tension (for thawed soils) and density. Equipment and test procedures (given in detail) were developed that allowed simulation in the laboratory of the gradual recovery of stiffness that occurs in the field after thawing. The resilient moduli were strongly dependent on soil state, dropping at least two orders of magnitude upon thawing. For all soils the moduli increased with increasing confining stress, generally decreased with increasing principal stress ratio, and increased with increasing moisture tension levels. The resilient moduli increased by a factor of approximately two as the materials recovered from the effects of a freeze-thaw cycle. The recovery process is well modeled as a function of soil moisture tension level. The stress sensitivity did not appear to be a function of the soil moisture tension level. The report also includes tabulations of all the repeated load triaxial test data.

  11. Characterization of mycobacterium isolates from pulmomary tuberculosis suspected cases visiting Tuberculosis Reference Laboratory at Ethiopian Health and Nutrition Research Institute, Addis Ababa Ethiopia:a cross sectional study

    Institute of Scientific and Technical Information of China (English)

    Biniam Mathewos; Nigatu Kebede; Tesfu Kassa; Adane Mihret; Muluwork Getahun

    2015-01-01

    Objective:To characterize mycobacterium isolates from pulmomary tuberculosis suspected cases visitingNationalTuberculosisReferenceLaboratory atEthiopianHealth andNutritionResearch Institute, for diagnosis of pulmonary tuberculosis fromJanuary4 toFebruary22,2010 with total samples of263.Methods:Sputum specimens were collected and processed; the deposits were cultured.For culturingLowensteinJensen medium(LJ) andMycobacteriaGrowthIndicatorTube (BACTECMGIT960) were used.CapiliaNeo was used for detectingNTM isolates from isolates of BACTECMGIT960.InArmauerHansenResearchInstitute,AddisAbabaEthiopia,Deletion typing PCR method for species identification(from confirmedMycobacterium tuberculosis complex (MTBC) isolates byCapiliaNeo) was done.Results:Out of263 enrolled in the study,124 and117 of them were positive for mycobacterium growth byBACTECMGIT960 andLJ culture method, respectively.FromBACTECMGIT960 positive media of124 isolates,117 were randomly taken to performCapiliaTBNeo test.From these7(6%) of them were found to beNTM and110(94%) were MTBC.From these110MTBC isolates,81 of them were randomly taken and run by the deletion typingRD9PCR method of molecular technique.Out of these78(96.3%) were found to be species ofMycobacterium tuberculosis and3(3.7%) were found to be not in theMTBC.Regarding the types of methods of culture media,MycobacteriaGrowthIndicatorTube(BACTECMGIT960) method was found to have excellent agreement(with kappa value of0.78) with the routine method of LJ.Conclusions:Pulmonary tuberculosis suspected cases visiting theNationalTuberculosis ReferenceLaboratory atEHNRI that were confirmed to be pulmonary tuberculosis are caused by the species ofMycobacterium tuberculosis, hence treatment regimen including pyrazinamide can be applied to the patients as the first choice in the study area inAddisAbaba,Ethiopia.There is indication of the presence ofNTM in patients visiting the tuberculosis reference laboratory and this is important becauseNTM is known to

  12. Biochemistry and evolutionary biology: Two disciplines that need each other

    Indian Academy of Sciences (India)

    Athel Cornish-Bowden; Juli Peretó; María Luz Cárdenas

    2014-03-01

    Biochemical information has been crucial for the development of evolutionary biology. On the one hand, the sequence information now appearing is producing a huge increase in the amount of data available for phylogenetic analysis; on the other hand, and perhaps more fundamentally, it allows understanding of the mechanisms that make evolution possible. Less well recognized, but just as important, understanding evolutionary biology is essential for understanding many details of biochemistry that would otherwise be mysterious, such as why the structures of NAD and other coenzymes are far more complicated than their functions would seem to require. Courses of biochemistry should thus pay attention to the essential role of evolution in selecting the molecules of life.

  13. Assessment of learning gains in a flipped biochemistry classroom.

    Science.gov (United States)

    Ojennus, Deanna Dahlke

    2016-01-01

    The flipped classroom has become an increasingly popular pedagogical approach to teaching and learning. In this study, learning gains were assessed in a flipped biochemistry course and compared to gains in a traditional lecture. Although measured learning gains were not significantly different between the two courses, student perception of learning gains did differ and indicates a higher level of satisfaction with the flipped lecture format.

  14. Proceedings of the NEANDC/NEACRP specialists meeting on fast neutron fission cross sections of U-233, U-235, U-238, and Pu-239, June 28--30, 1976, at Argonne National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Poenitz, W P; Guenther, P T

    1976-01-01

    Data files of all available data of absolute cross section measurements of U-233, U-235, U-238 and Pu-239, and of the ratios of U-233, U-238, and Pu-239 to U-235 were assembled at Argonne National Laboratory for use by the two Working Groups. The data files of absolute cross sections included also data measured relative to one of the standard cross sections H(n,n), Li-6(n,..cap alpha..), and B-10(n,..cap alpha..), and the ratio data files included ratios derived from absolute values which were measured in an identical type of experiment by the same group of experimenters. The subject files (e.g., U-235-Absolute, or U-238/U-235-Ratio, etc.) consisted of ''Sets.'' These sets contained the data from one experimental group which may have been published at different times. The assembling of the files was started with an extract from the CSISRS data files of the National Neutron Cross Section Center at the Brookhaven National Laboratory. Ratios were derived from quoted consistent sets of absolute cross sections, or from data which were actually measured as ratios but quoted as absolute values. The latter type of data was eliminated from the data files on absolute values. The files were improved by an extensive search for errors and data missing on the original CSISRS files at the time the extract was made. Other additions to the present subject files came from presentations made at this meeting and are described in the proceedings.

  15. Enhanced podcasts for teaching biochemistry to veterinary students.

    Science.gov (United States)

    Gough, Kevin C

    2011-01-01

    The teaching of biochemistry within medical disciplines presents certain challenges; firstly to relay a large body of complex facts and abstract concepts, and secondly to motivate students that this relatively difficult topic is worth their time to study. Here, nutrient biochemistry was taught within a multidisciplinary module as part of an undergraduate veterinary curriculum. The teaching approach was initially focussed on a mixture of didactic lectures and student-centred activities such as directed group/self learning. In subsequent years the core didactic lectures were replaced with enhanced podcasts covering the same material, along with the introduction of student presentations delivered within groups with both peer and facilitator assessment. These changes were accompanied by an increase in the time dedicated to this topic to allow sufficient time for students to work through podcasts and prepare presentations. The combination of these changes resulted in significant improvements in student performance within an in-course biochemistry long essay. These changes in the teaching approach, and particularly the introduction of extensive podcasts, was well received by students who perceived the process of going through the podcasts as time consuming but allowing them flexibility in both the pace that they studied this topic as well as the location and times that they studied it.

  16. Game Development as Didactic Strategy for Biochemistry Teaching

    Directory of Open Access Journals (Sweden)

    G.G. Hornink

    2010-05-01

    Full Text Available It is well known that students and teachers have difficulties in learning and teaching Biochemistry due to its abstract and interconnected contents. This work proposes a didactic strategy in order to facilitate teaching and learning process in Biochemistry. The strategy was implemented with biological science undergraduate students. At first, the students were divided into groups with a specific topic to develop a game. During the semester, problem based learning cases, online activities like crossword puzzle, essay questions and educational softwares were used to present the content of each topic. The groups were oriented in classroom and online, to choose and organize contents and create ways to approach them in games. At the end of the course the groups played each other games, which were evaluated by teacher and students following some criteria like: creativity, content organization, interdisciplinarity, proposal coherence, instructions clarity, specific content. The game elaboration contributed to the development of social and cognitive functions, such as teamwork and troubleshooting, providing an interesting perspective to the student about knowledge construction process. The strategy showed up students' creativity and ability to reorganize their knowledge to a different education level. In an overview, the results indicate that the proposed didactic strategy is an effective way to enhance learning and to motivate students into Biochemistry topics.

  17. THE USE OF MULTIPLE TOOLS FOR TEACHING MEDICAL BIOCHEMISTRY

    Directory of Open Access Journals (Sweden)

    A.B. Sé

    2007-05-01

    Full Text Available The pros and cons of Problem Based Learning (PBL have been extensivelydiscussed in the literature. We describe PBL-like strategies used at UnB (some ofthem since 1999 that may be useful elsewhere to improve undergraduatebiochemistry teaching with clinical applications. The main activities are: (i aseminar/poster system, (ii a true-or-false applied biochemistry exam (prepared bypeer tutors, (iii a 9-hour-exam on metabolism (based in actual papers, (iv anAdvanced Biochemistry course (directed to peer tutors, (v pizza-and-pasta (formetabolism teaching and free radicals (real science for students experiments,(vi the BioBio blog (http://www.biobio-unb.blogspot.com, (vii student lectures onhealth issues directed to the community, and (viii the BioBio Show. The mainobjective of these activities is providing students with a more practical andentertaining approach to biochemistry using philosophic PBL principles such asthe application of basic knowledge to real situations (diseases, experiments andscientific discoveries. We also emphasize (a the importance of peer-tutor activityfor optimized learning of students and peer tutors, (b the relevance of a closerinteraction between students and professors, and (c the necessity to initiatestudents precociously in actual basic/medical science and contact with the public.Most activities have been evaluated by the students through written questionnairesand informal conversations, for several semesters, indicating good acceptanceand approval of these methods.

  18. RCRA Facility Investigation report for Waste Area Grouping 6 at Oak Ridge National Laboratory, Oak Ridge, Tennessee. Volume 1, Sections 1 through 3: Environmental Restoration Program

    Energy Technology Data Exchange (ETDEWEB)

    1991-09-01

    WAG 6 comprises a shallow land burial facility used for disposal of low-level radioactive wastes (LLW) and, until recently, chemical wastes. As such, the site is subject to regulation under RCRA and the Comprehensive Environmental Response Compensation and Liability Act (CERCLA). To comply with these regulations, DOE, in conjunction with the Environmental Protection Agency (EPA) and the Tennessee Department of Environment and Conservation (TDEC), developed a strategy for closure and remediation of WAG 6 by 1997. A key component of this strategy was to complete an RFI by September 1991. The primary objectives of the RFI were to evaluate the site`s potential human health and environmental impacts and to develop a preliminary list of alternatives to mitigate these impacts. The WAG 6 one of three solid waste management units evaluated Oak Ridge National Laboratory (ORNL) existing waste disposal records and sampling data and performed the additional sampling and analysis necessary to: describe the nature and extent of contamination; characterize key contaminant transport pathways; and assess potential risks to human health and the environment by developing and evaluating hypothetical receptor scenarios. Estimated excess lifetime cancer risks as a result for exposure to radionuclides and chemicals were quantified for each hypothetical human receptor. For environmental receptors, potential impacts were qualitatively assessed. Taking into account regulatory requirements and base line risk assessment results, preliminary site closure and remediation objectives were identified, and a preliminary list of alternatives for site closure and remediation was developed.

  19. RCRA Facility Investigation report for Waste Area Grouping 6 at Oak Ridge National Laboratory, Oak Ridge, Tennessee. Volume 1. Sections 1 through 3

    Energy Technology Data Exchange (ETDEWEB)

    None

    1991-09-01

    WAG 6 comprises a shallow land burial facility used for disposal of low-level radioactive wastes (LLW) and, until recently, chemical wastes. As such, the site is subject to regulation under RCRA and the Comprehensive Environmental Response Compensation and Liability Act (CERCLA). To comply with these regulations, DOE, in conjunction with the Environmental Protection Agency (EPA) and the Tennessee Department of Environment and Conservation (TDEC), developed a strategy for closure and remediation of WAG 6 by 1997. A key component of this strategy was to complete an RFI by September 1991. The primary objectives of the RFI were to evaluate the site's potential human health and environmental impacts and to develop a preliminary list of alternatives to mitigate these impacts. The WAG 6 one of three solid waste management units evaluated Oak Ridge National Laboratory (ORNL) existing waste disposal records and sampling data and performed the additional sampling and analysis necessary to: describe the nature and extent of contamination; characterize key contaminant transport pathways; and assess potential risks to human health and the environment by developing and evaluating hypothetical receptor scenarios. Estimated excess lifetime cancer risks as a result for exposure to radionuclides and chemicals were quantified for each hypothetical human receptor. For environmental receptors, potential impacts were qualitatively assessed. Taking into account regulatory requirements and base line risk assessment results, preliminary site closure and remediation objectives were identified, and a preliminary list of alternatives for site closure and remediation was developed.

  20. Exclusion limits on the WIMP-nucleon cross section from the first run of the Cryogenic Dark Matter Search in the Soudan Underground Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Armel-Funkhouser, M.S.; /UC, Berkeley; Attisha, M.J.; /Case Western Reserve U.; Bailey, C.N.; /Case Western Reserve U.; Baudis, L.; /Florida U.; Bauer, Daniel A.; /Fermilab; Brink, P.L.; /Case Western Reserve U.; Bunker, R.; /UC, Santa Barbara; Cabrera, B.; /Case Western Reserve U.; Caldwell, D.O.; /UC, Santa Barbara; Chang, C.L.; /Case Western Reserve U.; Crisler, M.B.; /Fermilab; Cushman, P.; /Minnesota U.; Daal, M.; /UC, Berkeley; Dixon, R.; /Fermilab; Dragowsky, M.R.; Driscoll, D.D.; /Case Western Reserve U.; Duong, L.; /Minnesota U.; Ferril, R.; /UC, Santa Barbara; Filippini, J.; /UC, Berkeley; Gaitskell, R.J.; /Case Western Reserve U.; Hennings-Yeomans, R.; /Case Western Reserve U. /Fermilab /Case Western Reserve

    2005-07-01

    The Cryogenic Dark Matter Search (CDMS-II) employs low-temperature Ge and Si detectors to seek Weakly Interacting Massive Particles (WIMPs) via their elastic scattering interactions with nuclei. Simultaneous measurements of both ionization and phonon energy provide discrimination against interactions of background particles. For recoil energies above 10 keV, events due to background photons are rejected with > 99.99% efficiency. Electromagnetic events very near the detector surface can mimic nuclear recoils because of reduced charge collection, but these surface events are rejected with > 96% efficiency by using additional information from the phonon pulse shape. Efficient use of active and passive shielding, combined with the 2090 m.w.e. overburden at the experimental site in the Soudan mine, makes the background from neutrons negligible for this first exposure. All cuts are determined in a blind manner from in situ calibrations with external radioactive sources without any prior knowledge of the event distribution in the signal region. Resulting efficiencies are known to {approx}10%. A single event with a recoil of 64 keV passes all of the cuts and is consistent with the expected misidentification rate of surface-electron recoils. Under the assumptions for a standard dark matter halo, these data exclude previously unexplored parameter space for both spin-independent and spin-dependent WIMP-nucleon elastic scattering. The resulting limit on the spin-independent WIMP-nucleon elastic-scattering cross-section has a minimum of 4 x 10{sup -43} cm{sup 2} at a WIMP mass of 60 GeV c{sup -2}. The minimum of the limit for the spin-dependent WIMP-neutron elastic-scattering cross-section is 2 x 10{sup -37} cm{sup 2} at a WIMP mass of 50 GeV c{sup -2}.

  1. Are tutor-students capable of writing good biochemistry exams?

    Directory of Open Access Journals (Sweden)

    Sé Alexandre B.

    2004-05-01

    Full Text Available In a previous article we described the relevance of student seminars for the learning process of appliedbiochemistry for medical and nutrition students (Hermes-Lima et al., Biochem. Mol.Biol.Educ. 30:30-34,2002. First semester students of a basic biochemistry course (BioBio are divided in 10 groupsof 5 members, and each group is assigned to a specic topic (diabetes, cholesterol, etc under thesupervision of a tutor-student. The tutors have already coursed BioBio and are currently undertakingan advanced biochemistry course. In order to evaluate the learning of applied biochemistry for BioBiostudents a true or false exam (TFE is performed. This exam is made of 50 questions (5 on eachtopic elaborated by the tutors under the supervision of the teacher. The TFE corresponds to 10percent of the grade of BioBio and focus on clinical and/or applied biochemistry situations. At theend of the exam, BioBio students were asked to share their opinions about TFEs (n = 401, from2001/1 to 2003/2. When asked to give a 0-to-4 score regarding (a the diculty level of the test,(b the technical quality and (c if the exam makes an appropriate evaluation of applied biochemistryknowledge, the scores were 2.9, 3.4 and 2.9, respectively. BioBio students were also asked if they ndvalid to be evaluated by a tutor-made exam and if they would like to participate in the making ofTFEs; 96 and 58 percent answered yes, respectively.In another survey, we interviewed former BioBio students from the 2nd to the 7th semesters (n=95about TFEs (since 1999-1 regarding technical aspects, which included (1 clarity of questions, (2 levelof diculty, (3 clinical application and (4 thinking (as opposed to memorizing abilities demanded;the 0-to-4 scores were 3.1, 2.9, 2.6, and 2.5, respectively. Other four questions were on the validityof tutors writing TFEs and their capacity to perform such a task; the average score was 3.2. Oursurveys show the students good acceptance of the seminar system

  2. [Principles of cooperation between the specialties of internal medicine, pathology and clinical biochemistry].

    Science.gov (United States)

    Hölzel, W; Baumgarten, R; Fiedler, H; Zimmermann, S

    1985-12-01

    The optimal utilization of the knowledge and possibilities of pathological and clinical biochemistry presumes a close cooperation between it and the clinical specialties. The common working team of the GDR Society of Internal Medicine and the GDR Society for Clinical Chemistry and Laboratory Diagnostics makes theses of the central points of the cooperation in care, education, further education and postgraduate study and in research a subject for discussion. As essential tasks in the process of medical care are regarded the balance of the examination programme standing at the disposal, the establishment of diagnostic programmes, the establishment of organisational measures, the ascertainment of a use according to indication, the guarantee of the representance of examination material, the control of plausibility and the interpretation of test results. Since the realization of the tasks to a large extent depends on the cooperation of the specialities in education, further education and postgraduate study during the further education the clinician should become acquainted with the possibilities, the limits and the prerequisites for the performance of laboratory diagnostic investigations, the clinical biochemist with the problems of medical care and the value of the laboratory diagnosis in the total process of the treatment. In the field of research the result is a necessary cooperation in the clarification of patho-biochemical mechanisms, in the search for suitable laboratory diagnostic parameters for diagnostics and control of the course as well as in the statement of the validity of laboratory diagnostic parameters and parameter combinations taking into consideration the factors expenses, benefit and risk as well as further diagnostic possibilities.

  3. Hematological and Biochemistry Profile and Risk Factors Associated with Pulmonary Tuberculosis Patients in Guyana

    Directory of Open Access Journals (Sweden)

    Rajini Kurup

    2016-01-01

    Full Text Available Objective. To evaluate the hematological and biochemistry profile of patients with or without HIV-TB at the Georgetown Chest Clinic, Guyana. Methods. An observational, laboratory based study was designed to assess the relationship of PTB and HIV with patients routine biochemical and hematological values. The study was conducted during the period January 2013 to December 2014; a total sample size of 316 patients was enrolled following exclusion and inclusion criteria. Results. Mean age of study population was 40.1 ± 13.8 (95% CI 38.6–41.7 and most were between 40 and 49 age group (27.8%, 95% CI 23.2–33.0. More males were in the study 74.4% (95% CI 69.3–78.8 than females 81% (95% CI 21.1–30.7. 30% (95% CI 25.3–35.3 had a sputum smear grade of 3+ and 62.5% (95% CI 47.0–75.7 showed a CD4 count <200. The study demonstrated significantly low hemoglobin (Hb 91.7% (95% CI 78.2–97.1, low WBC 27.8% (95% CI 15.8–44.0, high indirect bilirubin 7.4% (95% CI 2.1–23.3, ALT 41.8% (95% CI 28.4–56.7, and AST 72.2% (95% CI 57.3–83.3 among TB-HIV patients. Homelessness RR (relative risk 2.2 (95% CI 0.48–12.3, smoking RR 1.09 (95% CI 1.01–1.19, and gender (male RR 1.2 (95% CI 0.61–2.26 were main associated risk factors. Conclusions. There is slight variation among PTB and PTB-HIV coinfected patients in some hematological and biochemistry parameters.

  4. Reference intervals for selected serum biochemistry analytes in cheetahs (Acinonyx jubatus)

    OpenAIRE

    Gavin C. Hudson-Lamb; Johan P. Schoeman; Emma H. Hooijberg; Sonja K. Heinrich; Tordiffe, Adrian S. W.

    2016-01-01

    Published haematologic and serum biochemistry reference intervals are very scarce for captive cheetahs and even more for free-ranging cheetahs. The current study was performed to establish reference intervals for selected serum biochemistry analytes in cheetahs. Baseline serum biochemistry analytes were analysed from 66 healthy Namibian cheetahs. Samples were collected from 30 captive cheetahs at the AfriCat Foundation and 36 free-ranging cheetahs from central Namibia. The effects of captivit...

  5. Light Imaging Section

    Data.gov (United States)

    Federal Laboratory Consortium — The mission of the Light Imaging Section is to give NIAMS scientists access to state-of-the-art light imaging equipment and to offer training and assistance at all...

  6. Flow Cytometry Section

    Data.gov (United States)

    Federal Laboratory Consortium — The primary goal of the Flow Cytometry Section is to provide the services of state-of-the-art multi-parameter cellular analysis and cell sorting for researchers and...

  7. Vertical integration of biochemistry and clinical medicine using a near-peer learning model.

    Science.gov (United States)

    Gallan, Alexander J; Offner, Gwynneth D; Symes, Karen

    2016-11-12

    Vertical integration has been extensively implemented across medical school curricula but has not been widely attempted in the field of biochemistry. We describe a novel curricular innovation in which a near-peer learning model was used to implement vertical integration in our medical school biochemistry course. Senior medical students developed and facilitated a case-based small group session for first year biochemistry students. Students were surveyed before and after the session on their attitudes about biochemistry, as well as the effectiveness of the session. Prior to the session, the students believed biochemistry was more important to understanding the basic science of medicine than it was to understanding clinical medicine or becoming a good physician. The session improved students' attitudes about the importance of biochemistry in clinical medicine, and after the session they now believe that understanding biochemistry is equally important to the basic sciences as clinical medicine. Students would like more sessions and believe the senior student facilitators were knowledgeable and effective teachers. The facilitators believe they improved their teaching skills. This novel combination of near-peer learning and vertical integration in biochemistry provided great benefit to both first year and senior medical students, and can serve as a model for other institutions. © 2016 by The International Union of Biochemistry and Molecular Biology, 44(6):507-516, 2016.

  8. The concept lens diagram: a new mechanism for presenting biochemistry content in terms of "big ideas".

    Science.gov (United States)

    Rowland, Susan L; Smith, Christopher A; Gillam, Elizabeth M A; Wright, Tony

    2011-07-01

    A strong, recent movement in tertiary education is the development of conceptual, or "big idea" teaching. The emphasis in course design is now on promoting key understandings, core competencies, and an understanding of connections between different fields. In biochemistry teaching, this radical shift from the content-based tradition is being driven by the "omics" information explosion; we can no longer teach all the information we have available. Biochemistry is a core, enabling discipline for much of modern scientific research, and biochemistry teaching is in urgent need of a method for delivery of conceptual frameworks. In this project, we aimed to define the key concepts in biochemistry. We find that the key concepts we defined map well onto the core science concepts recommended by the Vision and Change project. We developed a new method to present biochemistry through the lenses of these concepts. This new method challenged the way we thought about biochemistry as teachers. It also stimulated the majority of the students to think more deeply about biochemistry and to make links between biochemistry and material in other courses. This method is applicable to the full spectrum of content usually taught in biochemistry.

  9. HYDROMECHANICS LABORATORY

    Data.gov (United States)

    Federal Laboratory Consortium — Naval Academy Hydromechanics Laboratory The Naval Academy Hydromechanics Laboratory (NAHL) began operations in Rickover Hall in September 1976. The primary purpose...

  10. Bioassay Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Bioassay Laboratory is an accredited laboratory capable of conducting standardized and innovative environmental testing in the area of aquatic ecotoxicology. The...

  11. HYDROMECHANICS LABORATORY

    Data.gov (United States)

    Federal Laboratory Consortium — Naval Academy Hydromechanics LaboratoryThe Naval Academy Hydromechanics Laboratory (NAHL) began operations in Rickover Hall in September 1976. The primary purpose of...

  12. Comparative analysis of the biochemistry undergraduate courses in Brazil

    Directory of Open Access Journals (Sweden)

    P. A. Granjeiro

    2014-08-01

    Full Text Available INTRODUCTION: The economic and social development of Brazil during the recent decades has contributed to the installation of several new undergraduate and graduate study programs, as is the case of the undergraduate biochemistry programs at UFV, UFSJ and UEM. The new biochemical professionals are being prepared to work mainly in Industries, research Institutes, government agencies and Universities in all fields that involve Biochemistry and Molecular Biology. The aim of this study was to conduct a comparative analysis of the courses in Biochemistry in Brazil. MATERIAL AND METHODS: Comparative analysis of the course units of the UFV, UFSJ and UEM programs, centered on the curricula contents and organization and on the profiles of the students in terms of parameters such as the number of admissions and the graduation completion rates. RESULTS AND DISCUSSION: The UFV and UEM programs present a very similar distribution of workload over the biological, exact sciences, humanities, biochemical specialties and technological applications. The UFSJ program presents higher workloads in the areas of biological sciences and technological applications. No significant differences in the distribution of the workloads of mandatory and optional disciplines, complementary activities and supervised activities were detected. Over the past five years there was a decrease in the number of students that abandoned the programs, despite the increased retention time in the three courses. Most graduated students at both UFV and UFSJ continue their academic career toward the Master or Doctor degrees. CONCLUSION: Little difference between the study programs analyzed. This is somewhat surprising if one considers the fact that individual conception of each program was based on different local conditions and needs, which indeed justify small differences. The similarity of the programs, on the other hand, reflects the universality of the biochemical sciences and their broad

  13. TEACHING BIOCHEMISTRY USING EDUCATIONAL GAMES AND GAMIFICATION STRATEGIES

    Directory of Open Access Journals (Sweden)

    Yuri Rafael de Oliveira Silva

    2016-11-01

    Full Text Available INTRODUCTION: Biotechnology is a new bachelor degree in UFPA, and has been stablished with excellency in the state of Pará. However, there is the need to promote comprehension and learning in Biochemistry, as well as interdisciplinarity, that is an essential part of biotechnology. OBJECTIVES:  To increase learning and interdisciplinarity, educational games were used as tools. The students were instigated to develop educational games in different topics of energy metabolism. MATERIALS AND METHODS: The games were developed to be used in any teaching environment, since they were made with low-cost and accessible materials. This strategy was applied in three semesters in different Biochemistry classes, between 2012 and 2014. The best games in each class were used in following semesters. DISCUSSION AND RESULTS: Since the first semester, the failing rates dropped 15% compared to the previous semester, in which educational games were not used. An increase in learning (by observation could be noticed, including comprehension of metabolic pathways and their conections. Twenty games were developed in three semesters, and four of them are still being improved and used in other classes. The participant students answered a questionnaire, in which 47% defined the games as “Relaxing and Instigating”, 33% said the games “Accomplished their didactic and educational role” and 54% said they would recommend the use of these games as a reviewing activity. At the moment, another approach is being used to teach Biochemistry – Gamification, which uses elements found in games, as conflict, cooperation, rules and fun, to improve students’ motivation and engagement. CONCLUSION: As a partial result, there was greater in-class interest and engagement, better comprehension of the course content and the activities gave the students the opportunity to work in groups, to think critically about the themes and to develop opinions based on interdisciplinar and formal

  14. Construction of concept maps as tool for Biochemistry learning

    Directory of Open Access Journals (Sweden)

    Silvia Lopes de Menezes

    2006-07-01

    Full Text Available The use of concept maps on the teaching of sciences has been object of worldwide research with different purposes: to detect the previous knowledge of the students on certain topics or to evaluate learning, among others. Based on Ausubel´s cognitive psychology, concept maps assume that the learning is accomplished by assimilation of new concepts and propositions to the students´ cognitive structure, contributing to establish links between the previous and new knowledge. It is especially interesting on the approach of interdisciplinary issues, as many studied in Biochemistry.The relevance of the use of concept maps on biochemistry learning was evaluated on a thirty-hour undergraduation optional course, with interdisciplinary topics, which are not usually included on introductory Biochemistry courses. The course Biochemistry of Animal Venoms was structured in seven module where the biochemical action mechanisms of the venoms of Crotalus sp (south american rattlesnake, Bothrops sp (jararaca, Loxosceles sp (brown spider, Tityus sp (yellow scorpion, Phoneutria sp (armed spider, Apis mellifera (honey bee and Latrodectus sp (black widowwere discussed. The students worked in small groups and, at each module, there were (1 an oriented study, guided by questions, texts and schemes, supervised by the teachers, (2 the construction of individual concept maps, where the local and systemic effects of the venoms should be predicted by their biochemical composition and (3 the construction of a new map by the group, incorporating the information of the individual maps. The difficulty level of these tasks was gradually increased throughout the course, with lesser time to carry out the tasks, lesser assistance during the oriented study and even lesser information on the venom effects.The course assessment was given by the number, quality and correction of the concepts relationship present in the concept maps, through a questionnaire and by the

  15. Hartmut Lichtenthaler: an authority on chloroplast structure and isoprenoid biochemistry.

    Science.gov (United States)

    Sharkey, Thomas D; Govindjee

    2016-05-01

    We pay tribute to Hartmut Lichtenthaler for making important contributions to the field of photosynthesis research. He was recently recognized for ground-breaking discoveries in chloroplast structure and isoprenoid biochemistry by the Rebeiz Foundation for Basic Research (RFBR; http://vlpbp.org/ ), receiving a 2014 Lifetime Achievement Award for Photosynthesis. The ceremony, held in Champaign, Illinois, was attended by many prominent researchers in the photosynthesis field. We provide below a brief note on his education, and then describe some of the areas in which Hartmut Lichtenthaler has been a pioneer.

  16. German-Japanese relationships in biochemistry: a personal perspective

    Science.gov (United States)

    Sies, Helmut

    2016-01-01

    ABSTRACT The first Institute of Biochemistry in Japan was founded by Leonor Michaelis from Berlin at Nagoya in 1922, and there have been numerous interrelations between Japanese and German biochemists since. Some such relationships are presented here from a personal point of view as one illustrative example, which could be extended amply by the experience of many other scientists from the two countries. Fruitful exchanges are facilitated by organisations such as the Alexander von Humboldt Foundation (AvH) and the Deutscher Akademischer Austauschienst (DAAD) or the Japanese Society for the Promotion of Science (JSPS) and by the many bilateral agreements between universities and research institutions. PMID:28008189

  17. International Symposium on Insect Physiology, Biochemistry and Molecular Biology

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ We are building on the success of the Sixth Chinese Insect Physiology, Biochemistry and Molecular Biology Symposium, Beijing, held in 2005. The 2005 symposium saw many Chinese and international authorities share their expertise in a broad range of insect science, including analyses of insect genomes and proteomes, functional gene expression and regulation during development, insect immunity, insect neurobiology, insect-host interactions and insect chemical communication. The coming symposium, which will be held in Shandong University,Jinan, Shandong province, September 19-22, 2007, will offer material along similar lines.

  18. A Streamlined Western Blot Exercise: An Efficient and Greener Approach in the Laboratory Classroom

    Science.gov (United States)

    Ness, Traci L.; Robinson, Rebekah L.; Mojadedi, Wais; Peavy, Lydia; Weiland, Mitch H.

    2015-01-01

    SDS-PAGE and western blotting are two commonly taught protein detection techniques in biochemistry and molecular biology laboratory classrooms. A pitfall associated with incorporating these techniques into the laboratory is the significant wait times that do not allow students to obtain timely results. The waiting associated with SDS-PAGE comes…

  19. Brewing Beer in the Laboratory: Grain Amylases and Yeast's Sweet Tooth

    Science.gov (United States)

    Gillespie, Blake; Deutschman, William A.

    2010-01-01

    Brewing beer provides a straightforward and robust laboratory counterpart to classroom discussions of fermentation, a staple of the biochemistry curriculum. An exercise is described that provides several connections between lecture and laboratory content. Students first extract fermentable carbohydrates from whole grains, then ferment these with…

  20. Developing Technical Writing Skills in the Physical Chemistry Laboratory: A Progressive Approach Employing Peer Review

    Science.gov (United States)

    Gragson, Derek E.; Hagen, John P.

    2010-01-01

    Writing formal "journal-style" lab reports is often one of the requirements chemistry and biochemistry students encounter in the physical chemistry laboratory. Helping students improve their technical writing skills is the primary reason this type of writing is a requirement in the physical chemistry laboratory. Developing these skills is an…

  1. A national comparison of biochemistry and molecular biology capstone experiences.

    Science.gov (United States)

    Aguanno, Ann; Mertz, Pamela; Martin, Debra; Bell, Ellis

    2015-01-01

    Recognizing the increasingly integrative nature of the molecular life sciences, the American Society for Biochemistry and Molecular Biology (ASBMB) recommends that Biochemistry and Molecular Biology (BMB) programs develop curricula based on concepts, content, topics, and expected student outcomes, rather than courses. To that end, ASBMB conducted a series of regional workshops to build a BMB Concept Inventory containing validated assessment tools, based on foundational and discipline-specific knowledge and essential skills, for the community to use. A culminating activity, which integrates the educational experience, is often part of undergraduate molecular life science programs. These "capstone" experiences are commonly defined as an attempt to measure student ability to synthesize and integrate acquired knowledge. However, the format, implementation, and approach to outcome assessment of these experiences are quite varied across the nation. Here we report the results of a nation-wide survey on BMB capstone experiences and discuss this in the context of published reports about capstones and the findings of the workshops driving the development of the BMB Concept Inventory. Both the survey results and the published reports reveal that, although capstone practices do vary, certain formats for the experience are used more frequently and similarities in learning objectives were identified. The use of rubrics to measure student learning is also regularly reported, but details about these assessment instruments are sparse in the literature and were not a focus of our survey. Finally, we outline commonalities in the current practice of capstones and suggest the next steps needed to elucidate best practices.

  2. The Use of Case Studies in an Undergraduate Biochemistry Course

    Science.gov (United States)

    Cornely, Kathleen

    1998-04-01

    Most college biochemistry courses are taught in a format in which the professor lectures and the student memorizes. Although this is the best method for conveying large amounts of material, it puts the student in the position of passive learner. The lecture-based format has not been abandoned, but has been supplemented with case study projects assigned to the students upon completion of the intermediary metabolism unit. The case study assignment is modeled on similar exercises carried out in medical school biochemistry courses in the US and around the world. A description of the assignment follows: a group of 4-5 students is given a case study which gives the medical history of a patient with an inherited metabolic disease. The group is asked to provide biochemical explanations for the patient's symptoms and to suggest an effective course of treatment. The evaluation consists of a short paper that the students write as a group. The assignment provides the opportunity for small group interaction within a larger class and emphasizes cooperative-collaborative learning. Students learn by researching the topic on their own and debating it in small group discussions, and in so doing, gain a sense of confidence in themselves and the material they have learned over the course of the semester. Solving a "real-life" problem helps develop analytical and higher-order thinking skills and allows the students to see how biochemical concepts they have learned apply to a clinical situation.

  3. Integrating Responsible Conduct of Research Education into Undergraduate Biochemistry and Molecular Biology Laboratory Curricula

    Science.gov (United States)

    Hendrickson, Tamara L.

    2015-01-01

    Recently, a requirement for directed responsible conduct in research (RCR) education has become a priority in the United States and elsewhere. In the US, both the National Institutes of Health and the National Science Foundation require RCR education for all students who are financially supported by federal awards. The guidelines produced by these…

  4. Seed Storage Proteins as a System for Teaching Protein Identification by Mass Spectrometry in Biochemistry Laboratory

    Science.gov (United States)

    Wilson, Karl A.; Tan-Wilson, Anna

    2013-01-01

    Mass spectrometry (MS) has become an important tool in studying biological systems. One application is the identification of proteins and peptides by the matching of peptide and peptide fragment masses to the sequences of proteins in protein sequence databases. Often prior protein separation of complex protein mixtures by 2D-PAGE is needed,…

  5. Teaching Arrangements of Carbohydrate Metabolism in Biochemistry Curriculum in Peking University Health Science Center

    Science.gov (United States)

    Chen, Hao; Ni, Ju-Hua

    2013-01-01

    Biochemistry occupies a unique place in the medical school curricula, but the teaching of biochemistry presents certain challenges. One of these challenges is facilitating students' interest in and mastery of metabolism. The many pathways and modes of regulation can be overwhelming for students to learn and difficult for professors to teach…

  6. Experiences from introduction of peer-to-peer teaching methods in Advanced Biochemistry E2010

    DEFF Research Database (Denmark)

    Brodersen, Ditlev; Etzerodt, Michael; Rasmussen, Jan Trige

    2012-01-01

    During the autumn semester 2010, we experimented with a range of active teaching methods on the course, Advanced Biochemistry, at the Department of Molecular Biology and Genetics.......During the autumn semester 2010, we experimented with a range of active teaching methods on the course, Advanced Biochemistry, at the Department of Molecular Biology and Genetics....

  7. What Are the Appropriate Curriculum Contents for Biochemistry Courses in Veterinary Medicine?

    Science.gov (United States)

    Correia, A. A. D.; Correia, J. H. R. D.

    1995-01-01

    Presents an overview of the important items that the author's suggest should be included in a biochemistry course given to students in veterinary medicine. Presents a broad range of specific topics in biochemistry and strategies for covering as many topics as possible in one course. (LZ)

  8. Reactivity II: A Second Foundation-Level Course in Integrated Organic, Inorganic, and Biochemistry

    Science.gov (United States)

    Schaller, Chris P.; Graham, Kate J.; McIntee, Edward J.; Jones, T. Nicholas; Johnson, Brian J.

    2016-01-01

    A foundation-level course is described that integrates material related to reactivity in organic, inorganic, and biochemistry. Designed for second-year students, the course serves majors in chemistry, biochemistry, and biology, as well as prehealth-professions students. Building on an earlier course that developed concepts of nucleophiles and…

  9. Biochemistry Students' Ideas about How an Enzyme Interacts with a Substrate

    Science.gov (United States)

    Linenberger, Kimberly J.; Bretz, Stacey Lowery

    2015-01-01

    Enzyme-substrate interactions are a fundamental concept of biochemistry that is built upon throughout multiple biochemistry courses. Central to understanding enzyme-substrate interactions is specific knowledge of exactly how an enzyme and substrate interact. Within this narrower topic, students must understand the various binding sites on an…

  10. Teaching Arrangements of Carbohydrate Metabolism in Biochemistry Curriculum in Peking University Health Science Center

    Science.gov (United States)

    Chen, Hao; Ni, Ju-Hua

    2013-01-01

    Biochemistry occupies a unique place in the medical school curricula, but the teaching of biochemistry presents certain challenges. One of these challenges is facilitating students' interest in and mastery of metabolism. The many pathways and modes of regulation can be overwhelming for students to learn and difficult for professors to teach in an…

  11. Biochemistry Instructors' Views toward Developing and Assessing Visual Literacy in Their Courses

    Science.gov (United States)

    Linenberger, Kimberly J.; Holme, Thomas A.

    2015-01-01

    Biochemistry instructors are inundated with various representations from which to choose to depict biochemical phenomena. Because of the immense amount of visual know-how needed to be an expert biochemist in the 21st century, there have been calls for instructors to develop biochemistry students' visual literacy. However, visual literacy has…

  12. Case Study of How Turkish University Students Improve Their Biochemistry Achievement

    Science.gov (United States)

    Sadi, Özlem

    2013-01-01

    Biochemistry courses have an important place as a common subject in faculties of medicine, food engineering, biology and chemistry. MSLQ, Metacognitive Awareness Inventory and Learning Approach Questionnaire were used. The study also involves repeated observations of the same instructor in a biochemistry class over eight weeks to describe…

  13. Combining Content and Elements of Communication into an Upper-Level Biochemistry Course

    Science.gov (United States)

    Whittington, Carli P.; Pellock, Samuel J.; Cunningham, Rebecca L.; Cox, James R.

    2014-01-01

    This report describes how a science communication module was incorporated into an advanced biochemistry course. Elements of communication were taught synergistically with biochemistry content in this course in an effort to expose students to a variety of effective oral communication strategies. Students were trained to use these established…

  14. Cesarean Sections

    Science.gov (United States)

    ... Old Feeding Your 1- to 2-Year-Old Cesarean Sections (C-Sections) KidsHealth > For Parents > Cesarean Sections (C-Sections) A A A What's in this ... babies in the United States are delivered via cesarean section (C-section). Even if you're envisioning a ...

  15. The experimental teaching reform in biochemistry and molecular biology for undergraduate students in Peking University Health Science Center.

    Science.gov (United States)

    Yang, Xiaohan; Sun, Luyang; Zhao, Ying; Yi, Xia; Zhu, Bin; Wang, Pu; Lin, Hong; Ni, Juhua

    2015-01-01

    Since 2010, second-year undergraduate students of an eight-year training program leading to a Doctor of Medicine degree or Doctor of Philosophy degree in Peking University Health Science Center (PKUHSC) have been required to enter the "Innovative talent training project." During that time, the students joined a research lab and participated in some original research work. There is a critical educational need to prepare these students for the increasing accessibility of research experience. The redesigned experimental curriculum of biochemistry and molecular biology was developed to fulfill such a requirement, which keeps two original biochemistry experiments (Gel filtration and Enzyme kinetics) and adds a new two-experiment component called "Analysis of anti-tumor drug induced apoptosis." The additional component, also known as the "project-oriented experiment" or the "comprehensive experiment," consists of Western blotting and a DNA laddering assay to assess the effects of etoposide (VP16) on the apoptosis signaling pathways. This reformed laboratory teaching system aims to enhance the participating students overall understanding of important biological research techniques and the instrumentation involved, and to foster a better understanding of the research process all within a classroom setting. Student feedback indicated that the updated curriculum helped them improve their operational and self-learning capability, and helped to increase their understanding of theoretical knowledge and actual research processes, which laid the groundwork for their future research work.

  16. Fluorescence Quantum Yield Measurements of Fluorescent Proteins: A Laboratory Experiment for a Biochemistry or Molecular Biophysics Laboratory Course

    Science.gov (United States)

    Wall, Kathryn P.; Dillon, Rebecca; Knowles, Michelle K.

    2015-01-01

    Fluorescent proteins are commonly used in cell biology to assess where proteins are within a cell as a function of time and provide insight into intracellular protein function. However, the usefulness of a fluorescent protein depends directly on the quantum yield. The quantum yield relates the efficiency at which a fluorescent molecule converts…

  17. Diffusion Controlled Reactions, Fluctuation Dominated Kinetics, and Living Cell Biochemistry

    CERN Document Server

    Konkoli, Zoran

    2009-01-01

    In recent years considerable portion of the computer science community has focused its attention on understanding living cell biochemistry and efforts to understand such complication reaction environment have spread over wide front, ranging from systems biology approaches, through network analysis (motif identification) towards developing language and simulators for low level biochemical processes. Apart from simulation work, much of the efforts are directed to using mean field equations (equivalent to the equations of classical chemical kinetics) to address various problems (stability, robustness, sensitivity analysis, etc.). Rarely is the use of mean field equations questioned. This review will provide a brief overview of the situations when mean field equations fail and should not be used. These equations can be derived from the theory of diffusion controlled reactions, and emerge when assumption of perfect mixing is used.

  18. $^{31}$Mg $\\beta$-NMR applied in chemistry and biochemistry

    CERN Multimedia

    Magnesium ions, Mg$^{2+}$, are essential in biological systems, taking part in practically all phosphate chemistry, in photosynthesis as an integral component of chlorophyll, and they are regulated via transport through selective membrane proteins. Nonetheless, the function of magnesium ions in biochemistry is difficult to characterize, as it is practically invisible to current experimental techniques. With this proposal we aim to advance the use of $^{31}$Mg $\\beta$-NMR to liquid samples, building on the experience from the successful Letter of Intent INTC-I-088 “$\\beta$-NMR as a novel technique for biological applications”. Initially a series of experiments will be conducted aiming to characterize the coordination chemistry of Mg$^{2+}$ in ionic liquids (ILs), demonstrating that it is possible within the lifetime of the radioisotope to achieve binding of Mg$^{2+}$ to a molecule dissolved in the IL. ILs are chosen as they display a very low vapor pressure, and are thus straightforwardly compatible with t...

  19. Dissolution of hypotheses in biochemistry: three case studies.

    Science.gov (United States)

    Fry, Michael

    2016-12-01

    The history of biochemistry and molecular biology is replete with examples of erroneous theories that persisted for considerable lengths of time before they were rejected. This paper examines patterns of dissolution of three such erroneous hypotheses: The idea that nucleic acids are tetrads of the four nucleobases ('the tetranucleotide hypothesis'); the notion that proteins are collinear with their encoding genes in all branches of life; and the hypothesis that proteins are synthesized by reverse action of proteolytic enzymes. Analysis of these cases indicates that amassed contradictory empirical findings did not prompt critical experimental testing of the prevailing theories nor did they elicit alternative hypotheses. Rather, the incorrect models collapsed when experiments that were not purposely designed to test their validity exposed new facts.

  20. A focused assignment encouraging deep reading in undergraduate biochemistry.

    Science.gov (United States)

    Spiegelberg, Bryan D

    2014-01-01

    Encouraging undergraduate students to access, read, and analyze current primary literature can positively impact learning, especially in advanced courses. The incorporation of literature into coursework typically involves reading and responding to full research reports. Such exercises have clear value as students make connections between experiments and are able to probe and critique scientific logic. The exclusive use of full papers, though, may reinforce certain students' tendencies to rely on textual clues rather than a critical analysis of the actual data presented. I propose that structured activities requiring students to focus on individual parts of research papers, even on a single figure, are beneficial in a literature-centered advanced undergraduate course, because they promote the deep reading that is critical to scientific discourse. In addition, I describe how one such focused assignment boosted learning and was well received by students in a second-semester biochemistry course.

  1. Modelling Rho GTPase biochemistry to predict collective cell migration

    Science.gov (United States)

    Merchant, Brian; Feng, James

    The collective migration of cells, due to individual cell polarization and intercellular contact inhibition of locomotion, features prominently in embryogenesis and metastatic cancers. Existing methods for modelling collectively migrating cells tend to rely either on highly abstracted agent-based models, or on continuum approximations of the group. Both of these frameworks represent intercellular interactions such as contact inhibition of locomotion as hard-coded rules defining model cells. In contrast, we present a vertex-dynamics framework which predicts polarization and contact inhibition of locomotion naturally from an underlying model of Rho GTPase biochemistry and cortical mechanics. We simulate the interaction between many such model cells, and study how modulating Rho GTPases affects migratory characteristics of the group, in the context of long-distance collective migration of neural crest cells during embryogenesis.

  2. Development of a virtual classroom to teach biochemistry

    Directory of Open Access Journals (Sweden)

    R.S. Rodrigues

    2005-07-01

    Full Text Available Knowing  the  difficulties to  teach  some biochemistry concepts  because  of their  dynamic  and  spatial characteristics, computers  have been adopted  to help in these  visualizations.  Pictures, three  dimen- sional structures and animations were built and used to display in classes and distributed to students. Behind  these  specific illustrations, an  informatics  environment has  been  developed  to  support bio- chemistry  teaching.    Based  in  free software,  it fits  in  a single CD  that works  independent of any software installed  on the computer, even the operating  system, and is compatible  with most hardware configurations.This technique is called live-CD. It is based on Linux architecture, which is not only free software but also more flexible to be configured.  After some tests with Linux distributions, Slackware has been chosen because of its easy manipulation and  because it makes the  best use of the hardware  allowing to be installed  in old or limited  equipments. It has been configured to make the best optimization of the computer  and have all software needed for most biochemistry classrooms.It  was installed:   an  Internet browser  compatible  with  a 3D molecule visualization plug-in,  text editor,  presentation editor,  picture  editor  and  some didactic  material  specific for biochemistry.  The interface was configured for people with no experience in the Linux environment.The  system  can  also work in an  intranet, where  a computer  would  be operated  by the  teacher and it would have some special control configurations  as: web site access control, power control of the others  machines  and  even an option  that would bring  the  desktop  of other  machine  to the  teacher´s what  allows him to make a straight orientation for a student from his screen.This new system,  which is a common platform  for other

  3. Reflections on my career in analytical chemistry and biochemistry.

    Science.gov (United States)

    Sweeley, Charles C

    2010-01-01

    My career has been focused in two major areas, analytical chemistry and biochemistry of complex lipids and glycoconjugates. Included here are the pioneering work on the gas chromatography of long-chain sphingolipid bases, carbohydrates, steroids and urinary organic acids. Mass spectrometry was utilized extensively in structural studies of sphingolipids, fatty acids, carbohydrates, steroids, urinary organic acids, polyisoprenoid alcohols, and juvenile hormone. Computer systems were developed for the acquisition and analysis of mass spectra, and were used for development of automated metabolic profiling of complex mixtures of metabolites. Fabry's disease was discovered to be a glycosphingolipidosis. Enzymes of lysosomal metabolism of glycosphingolipids were purified, characterized, and used in one of the first demonstrations of the feasibility of enzyme replacement therapy in a lysosomal storage disorder (Fabry's disease). Extracellular sialidases were studied to evaluate the hypothesis that they might be involved in the regulation of membrane growth factor receptors. The enzyme for hematoside synthesis was purified and characterized.

  4. Oxidative stress in marine environments: biochemistry and physiological ecology.

    Science.gov (United States)

    Lesser, Michael P

    2006-01-01

    Oxidative stress-the production and accumulation of reduced oxygen intermediates such as superoxide radicals, singlet oxygen, hydrogen peroxide, and hydroxyl radicals-can damage lipids, proteins, and DNA. Many disease processes of clinical interest and the aging process involve oxidative stress in their underlying etiology. The production of reactive oxygen species is also prevalent in the world's oceans, and oxidative stress is an important component of the stress response in marine organisms exposed to a variety of insults as a result of changes in environmental conditions such as thermal stress, exposure to ultraviolet radiation, or exposure to pollution. As in the clinical setting, reactive oxygen species are also important signal transduction molecules and mediators of damage in cellular processes, such as apoptosis and cell necrosis, for marine organisms. This review brings together the voluminous literature on the biochemistry and physiology of oxidative stress from the clinical and plant physiology disciplines with the fast-increasing interest in oxidative stress in marine environments.

  5. Microfluidics: applications for analytical purposes in chemistry and biochemistry.

    Science.gov (United States)

    Ohno, Ken-ichi; Tachikawa, Kaoru; Manz, Andreas

    2008-11-01

    In this review, we present recent advancements and novel developments in fluidic systems for applied analytical purposes in chemistry, biochemistry, and life science in general that employ and reflect the full benefits of microfluidics. A staggering rise in publications related to integrated, all-in-one microfluidic chips capable of separation, reaction, and detection have been observed, all of which realise the principal of micro total analysis systems or lab-on-a-chip. These integrated chips actively adopt the scaling law concepts, utilising the highly developed fabrication techniques. Their aim is to multi-functionalise and fully automate devices believed to assist the future advancements of point-of-care, clinical, and medical diagnostics.

  6. DIABETES MELLITUS: GENERATING ISSUES FOR THE TEACHING OF BIOCHEMISTRY

    Directory of Open Access Journals (Sweden)

    Rodrigo Maciel Lima

    2016-11-01

    Full Text Available INTRODUCTION: Current education has been grounded on traditional teaching practices; in other words, learning is regarded as an accumulation of knowledge given by the teachers. Use of resources such as videos and games can raise the interest of teachers since they are an attractive and less traditional alternative. Nevertheless, the use of generating issues stands out as it may help teachers to develop contextualized lessons. According to Freire (1987, this is the starting point in the process of constructing knowledge, replacing traditional practices and questioning the student’s previous knowledge of Biochemistry. OBJECTIVES: Thus, the aim of this study was to prepare and present a lesson to a 12th grade class at IF Fluminense on carbohydrates, diabetes mellitus, and isomerism based on the theme “Diabetes Mellitus”. MATERIALS AND METHODS: In order to collect data and check the validity of the use of such methodology in classes of Biochemistry, we used procedures such as: presentation of a video made by the authors about diabetes, a styrofoam model of a hepatic cell and biscuit models to show its metabolic functioning regarding metabolism of carbohydrates, styrofoam and toothpick molecular models aimed at explaining isomerism among main hexoses and, to finish the process, a roulette game named “Spinning with Biochemistry”, adapted from the television show Roda a Roda Jequiti, presented by SBT network. In addition, students had a class based on the “Three Pedagogical Moments” methodology proposed by Delizoicov et al. (2007. DISCUSSION AND RESULTS: After this, students developed more grounded scientific concepts, making use of terms common in scientific language. This suggests that the use of a Generating Issues, in a class based on problem-solving methods supported by playful strategies, was a meaningful contribution to improve the understanding of scientific knowledge. CONCLUSION: This type of class grounded on less traditional

  7. STUDENTS’ DIFFICULTIES IN BIOCHEMISTRY LEARNING ANALYZED THROUGH AN ON LINE ACADEMIC DROP-IN CENTER

    Directory of Open Access Journals (Sweden)

    F. Schoenmaker

    2007-05-01

    Full Text Available The biochemistry discipline integrates the curriculum of all graduation courses onthe Biological Area and is a basis for other disciplines. The students’ difficulties inthis discipline are already widely recognized. To investigate these difficulties, wecreated a drop-in on line service that has two purposes: (1 To give support tostudents’ learning by answering their questions and solving their problemswhenever they appear and (2 to analyze the questions presented, as a strategy todiagnose the most prevalent difficulties. After two semesters of this service on line,217 questions were received and answered. There were few conceptual questionsbeing the majority related to problems and exercises. The most frequent questionsdealt with cell metabolism (54,4%, mainly lipid metabolism and aerobicmetabolism; basic concepts (11,5% such as about amino acids and buffer, proteinstructure (8,3% and enzymes (7,8%. These percentages are correlated to thenumber of hours dedicated to each subjects in the disciplines. The main difficultiesfounded were: integration of metabolic processes in different tissues, induction ofenergy reserve oxidation, reciprocal regulation between glycolysis andgluconeogenesis. We suppose that the lack of laboratory practice difficults thelearning of basic concepts. A more in-deep analysis will be necessary toinvestigate the causes of the pointed difficulties.

  8. Photometrics Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — Purpose:The Photometrics Laboratory provides the capability to measure, analyze and characterize radiometric and photometric properties of light sources and filters,...

  9. Target Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — [Part of the ATLAS user facility.] The Physics Division operates a target development laboratory that produces targets and foils of various thickness and substrates,...

  10. Blackroom Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: Enables evaluation and characterization of materials ranging from the ultraviolet to the longwave infrared (LWIR).DESCRIPTION: The Blackroom Laboratory is...

  11. Blackroom Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: Enables evaluation and characterization of materials ranging from the ultraviolet to the longwave infrared (LWIR). DESCRIPTION: The Blackroom Laboratory is...

  12. Minimal impact of organic chemistry prerequisite on student performance in introductory biochemistry.

    Science.gov (United States)

    Wright, Robin; Cotner, Sehoya; Winkel, Amy

    2009-01-01

    Curriculum design assumes that successful completion of prerequisite courses will have a positive impact on student performance in courses that require the prerequisite. We recently had the opportunity to test this assumption concerning the relationship between completion of the organic chemistry prerequisite and performance in introductory biochemistry. We found no statistically significant differences between average biochemistry grades or grade distribution among students with or without the organic chemistry prerequisite. However, students who had not completed the organic chemistry prerequisite before biochemistry were more likely to withdraw from the course than those who had completed the prerequisite. In contrast to the lack of correlation between performance in biochemistry and completion of organic chemistry, we observed a strong, highly significant positive relationship between cumulative GPA and the biochemistry grade. Our data suggest that excluding students without organic chemistry would have less positive impact on student success in biochemistry than would providing additional support for all students who enroll in biochemistry with a cumulative GPA below 2.5.

  13. Predictors of performance of students in biochemistry in a doctor of chiropractic curriculum.

    Science.gov (United States)

    Shaw, Kathy; Rabatsky, Ali; Dishman, Veronica; Meseke, Christopher

    2014-01-01

    Objective : This study investigated the effect of completion of course prerequisites, undergraduate grade point average (GPA), undergraduate degree, and study habits on the performance of students in the biochemistry course at Palmer College of Chiropractic Florida. Methods : Students self-reported information regarding academic preparation at the beginning of the semester using a questionnaire. Final exam grade and final course grade were noted and used as measures of performance. Multivariate analysis of variance was used to determine if number of prerequisites completed, undergraduate GPA, undergraduate degree, hours spent studying in undergraduate study, and hours spent studying in the first quarter of the chiropractic program were associated significantly with the biochemistry final exam grade or the final grade for the biochemistry course. Results : The number of prerequisites completed, undergraduate degree, hours spent studying in undergraduate study, and hours spent studying in the first quarter of the chiropractic program did not significantly affect the biochemistry final exam grade or the final grade for the biochemistry course, but undergraduate GPA did. Subsequent univariate analysis and Tukey's post hoc comparisons revealed that students with an undergraduate GPA in the 3.5 to 3.99 range earned significantly higher final course grades than students with an undergraduate GPA in the 2.5 to 2.99 range. Conclusion : No single variable was determined to be a factor that determines student success in biochemistry. The interrelationship between the factors examined warrants further investigation to understand fully how to predict the success of a student in the biochemistry course.

  14. Propaganda and Philanthropy: The Institute Bento da Rocha Cabral, the Lisbon Site of Biochemistry (1925-1953).

    Science.gov (United States)

    Carneiro, Ana; Amaral, Isabel

    2015-05-01

    This paper focuses on the internal organization and dynamics of the Institute Bento da Rocha Cabral (IRC) in Lisbon, a privately-funded institution devoted to biomedical research, from the particular vantage point of its laboratory of biochemistry; in particular, the process through which the institution turned from medically-related to chemically-related research in the period spanning from 1925 to 1953. The history of the IRC raises interesting questions regarding the social politics of science as it materialized the desire of leading physicians of the Faculty of Medicine of Lisbon to create proper physical facilities for medically-related scientific research. We argue that the process which led to the creation of the IRC coincided with the gradual professional and political ascendance of physicians in Portuguese society initiated in the late nineteenth century, and is closely associated with Portuguese republicanism and the process of Lisbon becoming the scientific capital.

  15. The use of software in Biochemistry teaching classes

    Directory of Open Access Journals (Sweden)

    M.B. Büttenbender

    2013-05-01

    Full Text Available INTRODUCTION: The rising of new technologies meant  to improve education could be considered a high advance to pedagogic methodologies. Software is defined as computer programs and may be considered educative when they present a methodology which assists and contextualizes the teaching-learning process. Specifically regarding Biochemistry, a knowledge area which explains physiological and pathological phenomena that occur in human beings, applying the use of software would turn out an easy way to observe suchphenomena. MATERIAL AND METHODS: In order to carry out this work, two free software designed to be used in Biochemistry area and developed at Universidade Estadual de Campinas (“Síntese Proteica” (Protein Synthesisand “A cinética da reação enzimática” (Kinetics of enzymatic reaction, were compared. Interface, how to work contents, advantages and disadvantages in the use of such kind of technology inside classroom were some of the evaluated parameters. DISCUSSION AND RESULTS: Both programs present a fine graphic design, allowing easy command comprehension. At the beginning the objectives of the programs and the contents they hold are presented, showing also a brief introduction to the topic. The programs also  present instruction manuals that explain how the experiments work. They are small basic and simple programs that run easily where they are placed, not needing internet access after their download. “Kinetics of enzymatic reaction” presented more interactive options than the other, and its operation could be considered more intuitive. CONCLUSION: We considered “Kinetics of enzymatic reaction” a better software,cause it allows the student to observe the experiment and perform the calculationsproposed, improving the learning process in a significantly way. The use ofnew technologies inside classrooms should be encouraged as a way to attractthe attention and interest of students, since they are

  16. CONSTRUCTIVISM APPLIED TO THE DISCIPLINE: A¨ DVANCED EDUCATION ON BIOCHEMISTRY

    Directory of Open Access Journals (Sweden)

    W. B. Maia

    2005-07-01

    Full Text Available Constructivism employs  models  that include  epistemological knowledge  and  strategies to  stimulate and motivate students so that they need to construct their own understanding of any subjetc  concept. Under  this  process,  the  primary  role of teaching  is not  to lecture,  explain,  or otherwise  attempt to´transferk´nowledge, but to create.  This principle was used in the Biochemistry Master Science Program of the  Universidade  Federal  de Pernambuco.  The  model applied  was divided  into  four phases:  (1 acquaintance of the student previous knowledge (using a pre-test; (2 presentation of the knowledge using a Science Teaching  CD-Rom;  (3 submission  of the  learned  content by the  student to teacher critical  analyis  and  (4  application of Interactionism (teacher/student/contents.   Each  phase  was individually  evaluated.  In the  first phase,  the  average  of correct  answers  of the  group  (13 students was 64%, whereas  the  second and  third  phases  presented  61% and  53%, respectively.   These  results reveal  a great  congnitive  unbalance  into  the  group.   They  also showed that the  previous  ideas and hypothesis  of the  students were in conflict  with  the  scientific  concepts.   After  teacher intervention (phase  four  the  group  presented  an increase  in the knowledge (86% of correct  answers.   Based  on these  results  one can conclude  that this  four-phase  model applied  and  based  on Constructivism  for acquiring  knowledge can be useful in Biochemistry  teaching.

  17. The evolution of the Krebs cycle: A promising subject for meaningful learning of biochemistry.

    Science.gov (United States)

    da Costa, Caetano; Galembeck, Eduardo

    2016-05-06

    Evolution has been recognized as a key concept for biologists. To enhance comprehension and motivate biology undergraduates for the contents of central energetic metabolism, we addressed the Krebs cycle structure and functions in an evolutionary view. To this end, we created a study guide that contextualizes the emergence of the cyclic pathway, in light of the prokaryotic influence since the early anaerobic condition of the Earth to increase oxygen in the atmosphere. The study guide is composed of three interrelated sections: (1) a problem, designed to arouse curiosity, inform and motivate students, (2) a text about life evolution, including early microorganisms and the emergence of the Krebs cycle, and (3) questions for debate. The activity consisted on individual reading and peer discussion based on this written material, under the guidance of the instructors. The questions were designed to foster debate in an ever-increasing level of complexity and to strengthen the main contextual aspects leading to emergence, evolving, and permanency of a complex metabolic pathway. Based on classroom observation, analysis of student's written responses, and individual interviews, we noticed they were engaged and motivated by the task, especially during group discussion. The whole experience suggests that the study guide was a stimulus to broaden the comprehension of the Krebs cycle, reinforcing the evolutionary approach as an important subject for learning purposes. © 2016 by The International Union of Biochemistry and Molecular Biology, 44:288-296, 2016.

  18. Interindividual variation, correlations, and sex-related differences in the salivary biochemistry of young healthy adults.

    Science.gov (United States)

    Prodan, Andrei; Brand, Henk S; Ligtenberg, Antoon J M; Imangaliyev, Sultan; Tsivtsivadze, Evgeni; van der Weijden, Fridus; Crielaard, Wim; Keijser, Bart J F; Veerman, Enno C I

    2015-06-01

    A cross-sectional observational study was conducted to evaluate interindividual biochemical variation in unstimulated whole saliva in a population of 268 systemically healthy young students, 18-30 yr of age, with no apparent caries lesions or periodontal disease. Salivary flow rate, protein content, pH, buffering capacity, mucins MUC5B and MUC7, albumin, secretory IgA, cystatin S, lactoferrin, chitinase, amylase, lysozyme, and proteases were measured using ELISAs and enzymatic activity assays. Significant differences were found between male and female subjects. Salivary pH, buffering capacity, protein content, MUC5B, secretory IgA, and chitinase activity were all lower in female subjects compared with male subjects, whereas MUC7 and lysozyme activity were higher in female subjects. There was no significant difference between sexes in salivary flow rate, albumin, cystatin S, amylase, and protease activity. Principal component analysis (PCA) and spectral clustering (SC) were used to assess intervariable relationships within the data set and to identify subgroups. Spectral clustering identified two clusters of participants, which were subsequently described. This study provides a comprehensive overview of the distribution and inter-relations of a set of important salivary biochemical variables in a systemically healthy young adult population, free of apparent caries lesions and periodontal disease. It highlights significant gender differences in salivary biochemistry.

  19. Learning Biochemistry through Manga--Helping Students Learn and Remember, and Making Lectures More Exciting.

    Science.gov (United States)

    Nagata, Ryoichi

    1999-01-01

    Uses panels taken from manga, Japanese comics and cartoons, to supplement explanations of biochemical terms and topics in biochemistry classes. Results indicate that the use of manga helped students remember what they had learned. (Author/CCM)

  20. Polish Academy of Sciences Institute of Biochemistry and Biophysics research report 1994-1995

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-31

    Scientific interests of Institute of Biochemistry and Biophysics Polish Academy of Sciences are focused on DNA replication and repair, gene expression, gene sequencing and molecular biophysics. The work reviews research projects of the Institute in 1994-1995.

  1. THE STRUCTURE, BIOCHEMISTRY, AND METABOLISM OF OSTEOARTHRITIC CARTILAGE - A REVIEW OF THE LITERATURE

    NARCIS (Netherlands)

    DIJKGRAAF, LC; DEBONT, LGM; BOER, G; LIEM, RSB

    1995-01-01

    Purpose: To understand the possible significance of the presence of proteases, cytokines, growth factors, and arachidonic acid metabolites in the osteoarthritic temporomandibular joint (TMJ), the pathogenesis of TMJ osteoarthritis (OA) is discussed, based on knowledge of structure, biochemistry and

  2. Minimal Impact of Organic Chemistry Prerequisite on Student Performance in Introductory Biochemistry

    OpenAIRE

    2009-01-01

    Curriculum design assumes that successful completion of prerequisite courses will have a positive impact on student performance in courses that require the prerequisite. We recently had the opportunity to test this assumption concerning the relationship between completion of the organic chemistry prerequisite and performance in introductory biochemistry. We found no statistically significant differences between average biochemistry grades or grade distribution among students with or without t...

  3. Technical and didactic problems of virtual lab exercises in biochemistry and biotechnology education

    DEFF Research Database (Denmark)

    May, Michael; Skriver, Karen; Dandanell, Gert

    from a lack of conceptual analysis of what actually constitutes virtual labs. A clarification of these conceptual issues is suggested as part of a Danish research and development project on virtual lab exercises in biochemistry, molecular biology and biotechnology education. The main outcome...... of this clarification is that specific didactic problems of biochemistry education can now be addressed through the design of exercises in the virtual lab environment....

  4. A synthetic biochemistry module for production of bio-based chemicals from glucose.

    Science.gov (United States)

    Opgenorth, Paul H; Korman, Tyler P; Bowie, James U

    2016-06-01

    Synthetic biochemistry, the cell-free production of biologically based chemicals, is a potentially high-yield, flexible alternative to in vivo metabolic engineering. To limit costs, cell-free systems must be designed to operate continuously with minimal addition of feedstock chemicals. We describe a robust, efficient synthetic glucose breakdown pathway and implement it for the production of bioplastic. The system's performance suggests that synthetic biochemistry has the potential to become a viable industrial alternative.

  5. Reflections on the Value of Mapping the Final Theory Examination in a Molecular Biochemistry Unit †

    OpenAIRE

    Rajaraman Eri; Anthony Cook; Natalie Brown

    2014-01-01

    This article assesses the impact of examination mapping as a tool to enhancing assessment and teaching quality in a second-year biochemistry unit for undergraduates. Examination mapping is a process where all questions in a written examination paper are assessed for links to the unit’s intended learning outcomes. We describe how mapping a final written examination helped visualise the impact of the assessment task on intended learning outcomes and skills for that biochemistry unit. The method...

  6. The physical basis of biochemistry the foundations of molecular biophysics

    CERN Document Server

    Bergethon, Peter R

    1998-01-01

    The objective of this book is to provide a unifying approach to the study of biophysical chemistry for the advanced undergraduate who has had a year of physics, organic chem­ istry, calculus, and biology. This book began as a revised edition of Biophysical Chemistry: Molecules to Membranes, which Elizabeth Simons and I coauthored. That short volume was written in an attempt to provide a concise text for a one-semester course in biophysical chemistry at the graduate level. The experience of teaching biophysical chemistry to bi­ ologically oriented students over the last decade has made it clear that the subject requires a more fundamental text that unifies the many threads of modem science: physics, chem­ istry, biology, mathematics, and statistics. This book represents that effort. This volume is not a treatment of modem biophysical chemistry with its rich history and many contro­ versies, although a book on that topic is also needed. The Physical Basis of Biochemistry is an introduction to the philosophy...

  7. Visual Literacy and Biochemistry Learning: The role of external representations

    Directory of Open Access Journals (Sweden)

    V.J.S.V. Santos

    2011-04-01

    Full Text Available Visual Literacy can bedefined as people’s ability to understand, use, think, learn and express themselves through external representations (ER in a given subject. This research aims to investigate the development of abilities of ERs reading and interpretation by students from a Biochemistry graduate course of theFederal University of São João Del-Rei. In this way, Visual Literacy level was  assessed using a questionnaire validatedin a previous educational research. This diagnosis questionnaire was elaborated according to six visual abilitiesidentified as essential for the study of the metabolic pathways. The initial statistical analysis of data collectedin this study was carried out using ANOVA method. Results obtained showed that the questionnaire used is adequate for the research and indicated that the level of Visual Literacy related to the metabolic processes increased significantly with the progress of the students in the graduation course. There was also an indication of a possible interference in the student’s performancedetermined by the cutoff punctuation in the university selection process.

  8. 42 CFR 493.1850 - Laboratory registry.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Laboratory registry. 493.1850 Section 493.1850... (CONTINUED) STANDARDS AND CERTIFICATION LABORATORY REQUIREMENTS Enforcement Procedures § 493.1850 Laboratory... laboratories, including the following: (1) A list of laboratories that have been convicted, under Federal...

  9. Computational Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — This laboratory contains a number of commercial off-the-shelf and in-house software packages allowing for both statistical analysis as well as mathematical modeling...

  10. Analytical Laboratories

    Data.gov (United States)

    Federal Laboratory Consortium — NETL’s analytical laboratories in Pittsburgh, PA, and Albany, OR, give researchers access to the equipment they need to thoroughly study the properties of materials...

  11. Laboratory Tests

    Science.gov (United States)

    Laboratory tests check a sample of your blood, urine, or body tissues. A technician or your doctor ... compare your results to results from previous tests. Laboratory tests are often part of a routine checkup ...

  12. Mother Earth Chemistry: A Laboratory Course for Nonmajors

    Science.gov (United States)

    Roberts, J. L.; Selco, J. I.; Wacks, D. B.

    1996-08-01

    Mother Earth Chemistry is a laboratory-based course designed to introduce nonscience majors to chemistry using familiar products such as beer, soap, yogurt, and cheese as well as products made from soybeans, including soymilk, tofu, and tempeh. Students make the products and learn some of the chemistry and biochemistry involved in their home manufacture as well as the rudiments of chemical analysis, stoichiometry, and the scientific method.

  13. A Biochemistry and Molecular Biology Experiment and Evaluation System for Biotechnology Specialty Students: An Effective Evaluation System to Improve the Biochemistry and Molecular Biology Experiment Teaching

    Science.gov (United States)

    Li, Suxia; Wu, Haizhen; Zhao, Jian; Ou, Ling; Zhang, Yuanxing

    2010-01-01

    In an effort to achieve high success in knowledge and technique acquisition as a whole, a biochemistry and molecular biology experiment was established for high-grade biotechnology specialty students after they had studied essential theory and received proper technique training. The experiment was based on cloning and expression of alkaline…

  14. Teaching Biochemistry at Lisbon University--Facing the Challenge of the Bologna Declaration in the 25th Anniversary of the Biochemistry Course

    Science.gov (United States)

    Farinha, Carlos M.; Freire, Ana Ponces

    2007-01-01

    The biochemistry degree has been taught at Lisbon University for 25 years. Since its creation, the curriculum is characterized for being widely eclectic and multidisciplinary. The adoption of the concepts proposed in Europe by the Declaration of Bologna and incorporation of these ideas at Lisbon University is discussed here for the biochemistry…

  15. Integrative Signaling Networks of Membrane Guanylate Cyclases: Biochemistry and Physiology

    Science.gov (United States)

    Sharma, Rameshwar K.; Duda, Teresa; Makino, Clint L.

    2016-01-01

    This monograph presents a historical perspective of cornerstone developments on the biochemistry and physiology of mammalian membrane guanylate cyclases (MGCs), highlighting contributions made by the authors and their collaborators. Upon resolution of early contentious studies, cyclic GMP emerged alongside cyclic AMP, as an important intracellular second messenger for hormonal signaling. However, the two signaling pathways differ in significant ways. In the cyclic AMP pathway, hormone binding to a G protein coupled receptor leads to stimulation or inhibition of an adenylate cyclase, whereas the cyclic GMP pathway dispenses with intermediaries; hormone binds to an MGC to affect its activity. Although the cyclic GMP pathway is direct, it is by no means simple. The modular design of the molecule incorporates regulation by ATP binding and phosphorylation. MGCs can form complexes with Ca2+-sensing subunits that either increase or decrease cyclic GMP synthesis, depending on subunit identity. In some systems, co-expression of two Ca2+ sensors, GCAP1 and S100B with ROS-GC1 confers bimodal signaling marked by increases in cyclic GMP synthesis when intracellular Ca2+ concentration rises or falls. Some MGCs monitor or are modulated by carbon dioxide via its conversion to bicarbonate. One MGC even functions as a thermosensor as well as a chemosensor; activity reaches a maximum with a mild drop in temperature. The complexity afforded by these multiple limbs of operation enables MGC networks to perform transductions traditionally reserved for G protein coupled receptors and Transient Receptor Potential (TRP) ion channels and to serve a diverse array of functions, including control over cardiac vasculature, smooth muscle relaxation, blood pressure regulation, cellular growth, sensory transductions, neural plasticity and memory.

  16. Construction of Hypertexts in a Biochemistry Pos- Graduation Discipline

    Directory of Open Access Journals (Sweden)

    W.B. Maia

    2009-05-01

    Full Text Available Virtual reality is an innovating manner of comprehending and acting on how the world is and, also, considered a new way of intellectual exercise.  This work took place in a  biochemistry masters discipline (Advanced Formation in ScientificEducation and had as its observation context the forum (on-line tool viability, intending the construction of hypertexts (active  collaborative writing by the 15 registered students in the  discipline in 2008. The discipline was available on the web, in  bioq.educacao.biz , where the students, teachers and monitors couldsubscribe. The virtual space was set with several environments (agenda,classroom, dictionary, email and forum; all of which were used during thediscipline. The forum, called orkuteducation, was destined to the hypertextelaboration, which was focused in three themes: 1º How to work with technology at school;   2º Teaching/learning methods and new information and communication technology; 3º Constructivism. The virtual learning environment had 2,275accesses to its content; being the forum the most visited one, with 1,026.   The built hypertext presented clear ideas about the approached themes, and realized the important role which a qualified teacher plays in the educational process. The new ways of create, organize and interact with information changes the relationship between the subject and the information itself. The hype rtext constitutes "high level computer tools", through which is possible to explore knowledge in a non -linear and interactive way. Hypertext remains a revolutionary concept oforganization and access to information and its generalization impact in society  is not known yet.

  17. THE POTENTIAL OF BIOCHEMISTRY EDUCATION APPS IN THE FORMATIVE ASSESSMENT

    Directory of Open Access Journals (Sweden)

    M. L. Oliveira

    2015-08-01

    Full Text Available Introduction and objectives: Apps can be designed to provide usage data, and most of them do. These data are usually used to map users interests and to deliver more effective ads that are more likely to result in clicks, and sales. We have applied some of these metrics to understand how can it be used to map students’ behavior and to promote a formative assessment using educational software. The purpose of a formative assessment is to monitor student learning to provide ongoing feedback that can be used by instructors and students to improve the teaching and learning process. Thus, this modality aims to help both students and instructors to identify strengths and weaknesses that need to be developed. This study aimed to describe the potential of educational apps in the formative assessment process. Material and Methods: We have implemented assessment tools embedded in three apps (ARMET, The Cell and 3D Class used to teach: 1 Metabolic Pathways; 2 Scale of the cellular structures, and 3 Concepts from techniques used in a Biochemistry Lab course. The implemented tools allow to verify on what issues there were recurring mistakes, the total number of mistakes presented, which questions they most achieved, how long they took to perform the activity and other relevant information. Results and conclusion: Educational apps can provide transparent and coherent evaluation metrics to enable instructors to systematize more consistent criteria and indicators, reducing the subjectivity of the formative assessment process and the time spent for preparation, tabulation and analysis of assessment data. This approach allows instructors to understand better where students struggle, giving to them a more effective feedback. It also helps instructor to plan interventions to help students to perform better and to achieve the learning objectives.

  18. Designing Polymerase Chain Reaction (PCR) Primer Multiplexes in the Forensic Laboratory

    Science.gov (United States)

    Elkins, Kelly M.

    2011-01-01

    The polymerase chain reaction (PCR) is a common experiment in upper-level undergraduate biochemistry, molecular biology, and forensic laboratory courses as reagents and thermocyclers have become more affordable for institutions. Typically, instructors design PCR primers to amplify the region of interest and the students prepare their samples for…

  19. Dipeptide Structural Analysis Using Two-Dimensional NMR for the Undergraduate Advanced Laboratory

    Science.gov (United States)

    Gonzalez, Elizabeth; Dolino, Drew; Schwartzenburg, Danielle; Steiger, Michelle A.

    2015-01-01

    A laboratory experiment was developed to introduce students in either an organic chemistry or biochemistry lab course to two-dimensional nuclear magnetic resonance (2D NMR) spectroscopy using simple biomolecules. The goal of this experiment is for students to understand and interpret the information provided by a 2D NMR spectrum. Students are…

  20. Purification and Characterization of Enzymes from Yeast: An Extended Undergraduate Laboratory Sequence for Large Classes

    Science.gov (United States)

    Johanson, Kelly E.; Watt, Terry J.; McIntyre, Neil R.; Thompson, Marleesa

    2013-01-01

    Providing a project-based experience in an undergraduate biochemistry laboratory class can be complex with large class sizes and limited resources. We have designed a 6-week curriculum during which students purify and characterize the enzymes invertase and phosphatase from bakers yeast. Purification is performed in two stages via ethanol…

  1. Simple Laboratory Exercise for Induction of Beta-Mannanase from "Aspergillus niger"

    Science.gov (United States)

    Mulimani, V. H.; Naganagouda, K.

    2010-01-01

    This laboratory experiment was designed for Biochemistry, Biotechnology, Microbiology, and Food Technology students of undergraduate and postgraduate courses. The experiment shows the advantages of using agricultural waste, copra mannan as potent inducer of [beta]-mannanase. The students were able to compare the enzyme induction by commercial…

  2. Impact of e-resources on learning in biochemistry: first-year medical students’ perceptions

    Directory of Open Access Journals (Sweden)

    Varghese Joe

    2012-05-01

    Full Text Available Abstract Background E-learning resources (e-resources have been widely used to facilitate self-directed learning among medical students. The Department of Biochemistry at Christian Medical College (CMC, Vellore, India, has made available e-resources to first-year medical students to supplement conventional lecture-based teaching in the subject. This study was designed to assess students’ perceptions of the impact of these e-resources on various aspects of their learning in biochemistry. Methods Sixty first-year medical students were the subjects of this study. At the end of the one-year course in biochemistry, the students were administered a questionnaire that asked them to assess the impact of the e-resources on various aspects of their learning in biochemistry. Results Ninety-eight percent of students had used the e-resources provided to varying extents. Most of them found the e-resources provided useful and of a high quality. The majority of them used these resources to prepare for periodic formative and final summative assessments in the course. The use of these resources increased steadily as the academic year progressed. Students said that the extent to which they understood the subject (83% and their ability to answer questions in assessments (86% had improved as a result of using these resources. They also said that they found biochemistry interesting (73% and felt motivated to study the subject (59%. Conclusions We found that first-year medical students extensively used the e-resources in biochemistry that were provided. They perceived that these resources had made a positive impact on various aspects of their learning in biochemistry. We conclude that e-resources are a useful supplement to conventional lecture-based teaching in the medical curriculum.

  3. Laboratory Building.

    Energy Technology Data Exchange (ETDEWEB)

    Herrera, Joshua M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-03-01

    This report is an analysis of the means of egress and life safety requirements for the laboratory building. The building is located at Sandia National Laboratories (SNL) in Albuquerque, NM. The report includes a prescriptive-based analysis as well as a performance-based analysis. Following the analysis are appendices which contain maps of the laboratory building used throughout the analysis. The top of all the maps is assumed to be north.

  4. Laboratory Building

    Energy Technology Data Exchange (ETDEWEB)

    Herrera, Joshua M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-03-01

    This report is an analysis of the means of egress and life safety requirements for the laboratory building. The building is located at Sandia National Laboratories (SNL) in Albuquerque, NM. The report includes a prescriptive-based analysis as well as a performance-based analysis. Following the analysis are appendices which contain maps of the laboratory building used throughout the analysis. The top of all the maps is assumed to be north.

  5. Dynamics Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Dynamics Lab replicates vibration environments for every Navy platform. Testing performed includes: Flight Clearance, Component Improvement, Qualification, Life...

  6. Visualization Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: Evaluates and improves the operational effectiveness of existing and emerging electronic warfare systems. By analyzing and visualizing simulation results...

  7. Propulsion Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Propulsion Lab simulates field test conditions in a controlled environment, using standardized or customized test procedures. The Propulsion Lab's 11 cells can...

  8. Chemistry Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — Purpose: To conduct fundamental studies of highway materials aimed at understanding both failure mechanisms and superior performance. New standard test methods are...

  9. Psychology Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — This facility provides testing stations for computer-based assessment of cognitive and behavioral Warfighter performance. This 500 square foot configurable space can...

  10. Analytical Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Analytical Labspecializes in Oil and Hydraulic Fluid Analysis, Identification of Unknown Materials, Engineering Investigations, Qualification Testing (to support...

  11. Implementation of a Collaborative Series of Classroom-Based Undergraduate Research Experiences Spanning Chemical Biology, Biochemistry, and Neurobiology.

    Science.gov (United States)

    Kowalski, Jennifer R; Hoops, Geoffrey C; Johnson, R Jeremy

    2016-01-01

    Classroom undergraduate research experiences (CUREs) provide students access to the measurable benefits of undergraduate research experiences (UREs). Herein, we describe the implementation and assessment of a novel model for cohesive CUREs focused on central research themes involving faculty research collaboration across departments. Specifically, we implemented three collaborative CUREs spanning chemical biology, biochemistry, and neurobiology that incorporated faculty members' research interests and revolved around the central theme of visualizing biological processes like Mycobacterium tuberculosis enzyme activity and neural signaling using fluorescent molecules. Each CURE laboratory involved multiple experimental phases and culminated in novel, open-ended, and reiterative student-driven research projects. Course assessments showed CURE participation increased students' experimental design skills, attitudes and confidence about research, perceived understanding of the scientific process, and interest in science, technology, engineering, and mathematics disciplines. More than 75% of CURE students also engaged in independent scientific research projects, and faculty CURE contributors saw substantial increases in research productivity, including increased undergraduate student involvement and academic outputs. Our collaborative CUREs demonstrate the advantages of multicourse CUREs for achieving increased faculty research productivity and traditional CURE-associated student learning and attitude gains. Our collaborative CURE design represents a novel CURE model for ongoing laboratory reform that benefits both faculty and students.

  12. On the Chemical Emergence of Phosphate-Based Biochemistry

    Science.gov (United States)

    Kee, Terence

    Contemporary organisms use orthophosphate derivatives (PO43-) in their cell biochemistry,1 yet questions remain as to how Nature was able to accumulate, activate and exploit the or-thophosphate group from geological sources with both poorly solubility and low chemical activ-ity.2 Gulick argued3 a central role for reduced oxidation state phosphorus (P) oxyacids such as H-phosphonates (H2PO3-) and especially H-phosphinates (H2PO2-) in prebiotic chemistry on account of the greater water solubility of their metal salts and, with the presence of P-H bonds, a different reactivity profile to that expected of orthophosphate. The recent demonstration that hydrothermal corrosion of P-rich mineral phases such as schreibersite (Fe,Ni)3P within iron meteorites leads to production of various P-oxyacids including H-phosphonic (H3PO3)4 and H-phosphinic5 acids as well as orthophosphate has reignited interest in reduced oxida-tion state P chemistry in prebiotic environments. We are examining the prebiotic potential of reduced oxidation state P-chemistry through reactions with carbonyl substrates with rea-sonable prebiotic provenance including formaldehyde glycolaldehyde, both intimately involved in the formose reaction for sugar synthesis6 and pyruvic acid,7 a product of glycolysis and feed-stock for the citric acid cycle, a fundamental cellular metablic process whose heritage is considered an ancient one. In this contribution we present some of our latest results on the H-phosphinate-pyruvate system. References: [1] Lodish H et al. (2000) Molecular Cell Biology, 4th Ed., W. H. Freeman Co., New York. [2] Gulick A. (1955) Am. Sci., 43, 479. [3] Gulick A. (1957) Ann. N. Y. Acad. Sci. 69, 309. [4] Pasek M. A. (2008) Proc. Nat. Acad. Sci. USA, 105, 853. [5] Bryant D. E.and Kee T. P. (2006) Chem. Commun. 2344. [6] Weber A. L. (2000) Origins of Life and Evol. Biosph., 30, 33. [7] Cody G. D. et. al. (2000) Science 289, 1337.

  13. Learning Laboratory.

    Science.gov (United States)

    Hay, Lyn; Callison, Daniel

    2000-01-01

    Considers the school library media center as an information learning laboratory. Topics include information literacy; Kuhlthau's Information Search Process model; inquiry theory and approach; discovery learning; process skills of laboratory science; the information scientist; attitudes of media specialists, teachers, and students; displays and Web…

  14. A synthetic biochemistry molecular purge valve module that maintains redox balance.

    Science.gov (United States)

    Opgenorth, Paul H; Korman, Tyler P; Bowie, James U

    2014-06-17

    The greatest potential environmental benefit of metabolic engineering would be the production of high-volume commodity chemicals, such as biofuels. Yet, the high yields required for the economic viability of low-value chemicals is particularly hard to achieve in microbes owing to the myriad competing biochemical pathways. An alternative approach, which we call synthetic biochemistry, is to eliminate the organism by constructing biochemical pathways in vitro. Viable synthetic biochemistry, however, will require simple methods to replace the cellular circuitry that maintains cofactor balance. Here we design a simple purge valve module for maintaining NADP(+)/NADPH balance. We test the purge valve in the production of polyhydroxybutyryl bioplastic and isoprene--pathways where cofactor generation and utilization are unbalanced. We find that the regulatory system is highly robust to variations in cofactor levels and readily transportable. The molecular purge valve provides a step towards developing continuously operating, sustainable synthetic biochemistry systems.

  15. Scientific literature on teaching strategies used in biochemistry courses: a systematic review

    Directory of Open Access Journals (Sweden)

    Joice Trindade Silveira

    2016-12-01

    Full Text Available The aim of this study was to know teaching strategies undertaken by biochemistry teachers in higher education. A systematic review of the literature was conducted, and was included articles developed in Brazilian universities, published in the 2004-2015 period, with descriptors learning, biochemistry and graduation. 40 publications were found, and 2004 was the year with most studies (n=7. The most common activities were conducting experimental activity (32,5%, development of various teaching tools (22,5%, use of Problem-based Learning (20%, use of information technologies (17,5% and development courses (7,5%. Thus, it was observed that there are groups of teachers working with different teaching strategies seeking to improve student learning. The production and publication of knowledge in teaching in biochemistry can help this process.

  16. Biochemistry students' ideas about shape and charge in enzyme-substrate interactions.

    Science.gov (United States)

    Linenberger, Kimberly J; Bretz, Stacey Lowery

    2014-01-01

    Biochemistry is a visual discipline that requires students to develop an understanding of numerous representations. However, there is very little known about what students actually understand about the representations that are used to communicate ideas in biochemistry. This study investigated biochemistry students' understanding of multiple representations of enzyme-substrate interactions through both student interviews (N = 25) and responses by a national sample (N = 707) to the Enzyme-Substrate Interactions Concept Inventory. This manuscript reports the findings regarding one category of misconceptions measured by the concept inventory, namely, students' understandings of shape and charge in the context of enzyme-substrate interactions. Students interpret molecular representations depicting such interactions by determining the complementarity between enzyme and substrate by focusing upon charge and hydrogen bonding, but with a disregard for stereochemistry.

  17. The biochemistry of blister fluid from pediatric burn injuries: proteomics and metabolomics aspects.

    Science.gov (United States)

    Zang, Tuo; Broszczak, Daniel A; Broadbent, James A; Cuttle, Leila; Lu, Haitao; Parker, Tony J

    2016-01-01

    Burn injury is a prevalent and traumatic event for pediatric patients. At present, the diagnosis of burn injury severity is subjective and lacks a clinically relevant quantitative measure. This is due in part to a lack of knowledge surrounding the biochemistry of burn injuries and that of blister fluid. A more complete understanding of the blister fluid biochemistry may open new avenues for diagnostic and prognostic development. Burn insult induces a highly complex network of signaling processes and numerous changes within various biochemical systems, which can ultimately be examined using proteome and metabolome measurements. This review reports on the current understanding of burn wound biochemistry and outlines a technical approach for 'omics' profiling of blister fluid from burn wounds of differing severity.

  18. Approaches to enhance the teaching quality of experimental biochemistry for MBBS students in TSMU, China.

    Science.gov (United States)

    Yu, Lijuan; Yi, Shuying; Zhai, Jing; Wang, Zhaojin

    2017-02-23

    With the internationalization of medical education in China, the importance of international students' education in medical schools is also increasing. Except foreign students majoring in Chinese language, English Bachelor of Medicine, Bachelor of Surgery (MBSS) students are the largest group of international students. Based on problems in the teaching process for experimental biochemistry, we designed teaching models adapted to the background of international students and strengthened teachers' teaching ability at Taishan Medical University. Several approaches were used in combination to promote teaching effects and increase the benefit of teaching to teachers. The primary data showed an increased passion for basic medical biochemistry and an improved theoretical background for MBSS students, which will be helpful for their later clinical medicine studies. © 2017 by The International Union of Biochemistry and Molecular Biology, 2017.

  19. Concept Maps in Teaching of Biochemistry, an Integration between Scientific Concepts

    Directory of Open Access Journals (Sweden)

    Denise Braga Schimidt

    2014-10-01

    Full Text Available The contents of Biochemistry discipline require previous scientific knowledge and it is often disconnected and fragmented, voiding a meaningful learning. This article describes an experience with the use of an alternative methodology to teach Biochemistry: Concept Maps. The procedural design was based on the use of subject questionnaires before and after reading the Concept Map developed from a subject studied in Biochemistry during the graduation program for the dental school of UFF (RJ, Brazil. Integration of Metabolism is consensus among teachers as a topic able to effectively correlate different concepts as it has been chosen to prepare the Map. The aim of this study was to show this teaching tool that might allow concepts’ integration. The results showed, after using the Map, a higher number of students getting right answers to all proposed questions, suggesting improvement in Significant Learning.

  20. Laboratory animal allergy.

    NARCIS (Netherlands)

    Hollander, A.

    1997-01-01

    The main objective of the study presented in this thesis was to estimate the prevalence rate of laboratory animal allergy and to determine its association with risk factors, like allergen exposure level, atopy, gender and other host factors. A cross-sectional survey was undertaken among 540 workers

  1. Essential concepts and underlying theories from physics, chemistry, and mathematics for "biochemistry and molecular biology" majors.

    Science.gov (United States)

    Wright, Ann; Provost, Joseph; Roecklein-Canfield, Jennifer A; Bell, Ellis

    2013-01-01

    Over the past two years, through an NSF RCN UBE grant, the ASBMB has held regional workshops for faculty members from around the country. The workshops have focused on developing lists of Core Principles or Foundational Concepts in Biochemistry and Molecular Biology, a list of foundational skills, and foundational concepts from Physics, Chemistry, and Mathematics that all Biochemistry or Molecular Biology majors must understand to complete their major coursework. The allied fields working group created a survey to validate foundational concepts from Physics, Chemistry, and Mathematics identified from participant feedback at various workshops. One-hundred twenty participants responded to the survey and 68% of the respondents answered yes to the question: "We have identified the following as the core concepts and underlying theories from Physics, Chemistry, and Mathematics that Biochemistry majors or Molecular Biology majors need to understand after they complete their major courses: 1) mechanical concepts from Physics, 2) energy and thermodynamic concepts from Physics, 3) critical concepts of structure from chemistry, 4) critical concepts of reactions from Chemistry, and 5) essential Mathematics. In your opinion, is the above list complete?" Respondents also delineated subcategories they felt should be included in these broad categories. From the results of the survey and this analysis the allied fields working group constructed a consensus list of allied fields concepts, which will help inform Biochemistry and Molecular Biology educators when considering the ASBMB recommended curriculum for Biochemistry or Molecular Biology majors and in the development of appropriate assessment tools to gauge student understanding of how these concepts relate to biochemistry and molecular biology.

  2. Section on High Resolution Optical Imaging (HROI)

    Data.gov (United States)

    Federal Laboratory Consortium — The Section on High Resolution Optical Imaging (HROI) develops novel technologies for studying biological processes at unprecedented speed and resolution. Research...

  3. 21 CFR 226.58 - Laboratory controls.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false Laboratory controls. 226.58 Section 226.58 Food... Laboratory controls. Laboratory controls shall include the establishment of adequate specifications and test... standards of identity, strength, quality, and purity. Laboratory controls shall include: (a)...

  4. 21 CFR 211.194 - Laboratory records.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false Laboratory records. 211.194 Section 211.194 Food... Laboratory records. (a) Laboratory records shall include complete data derived from all tests necessary to..., including all graphs, charts, and spectra from laboratory instrumentation, properly identified to show...

  5. 42 CFR 441.17 - Laboratory services.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Laboratory services. 441.17 Section 441.17 Public... Provisions § 441.17 Laboratory services. (a) The plan must provide for payment of laboratory services as defined in § 440.30 of this subchapter if provided by— (1) An independent laboratory that meets...

  6. 7 CFR 983.1 - Accredited laboratory.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Accredited laboratory. 983.1 Section 983.1 Agriculture..., ARIZONA, AND NEW MEXICO Definitions § 983.1 Accredited laboratory. An accredited laboratory is a laboratory that has been approved or accredited by the U.S. Department of Agriculture....

  7. 40 CFR 60.535 - Laboratory accreditation.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Laboratory accreditation. 60.535... Wood Heaters § 60.535 Laboratory accreditation. (a)(1) A laboratory may apply for accreditation by the... Heater Laboratory Accreditation. (2) (3) If accreditation is denied under this section, the...

  8. 21 CFR 606.140 - Laboratory controls.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Laboratory controls. 606.140 Section 606.140 Food... CURRENT GOOD MANUFACTURING PRACTICE FOR BLOOD AND BLOOD COMPONENTS Laboratory Controls § 606.140 Laboratory controls. Laboratory control procedures shall include: (a) The establishment of...

  9. The Gran Sasso Laboratory

    Science.gov (United States)

    Votano, L.

    2012-09-01

    The Gran Sasso underground laboratory is one of the four national laboratories run by the INFN (Istituto Nazionale di Fisica Nucleare). It is located under the Gran Sasso massif, in central Italy, between the cities of L'Aquila and Teramo, 120 km far from Rome. It is the largest underground laboratory for astroparticle physics in the world and the most advanced in terms of complexity and completeness of its infrastructures. The scientific program at the Gran Sasso National Laboratories (Laboratori Nazionali del Gran Sasso, LNGS)is mainly focused on astroparticle, particle and nuclear physics. The laboratory presently hosts many experiments as well as R&D activities, including world-leading research in the fields of solar neutrinos, accelerator neutrinos (CNGS neutrino beam from CERN to Gran Sasso), dark matter, neutrinoless double-beta decay and nuclear cross-section of astrophysical interest. Associate sciences like earth physics, biology and fundamental physics complement the activities. The laboratory is operated as an international science facility and hosts experiments whose scientific merit is assessed by an international advisory Scientific Committee. A review of the main experiments carried out at LNGS will be given, together with the most recent and relevant scientific results achieved.

  10. Diverse Assessment and Active Student Engagement Sustain Deep Learning: A Comparative Study of Outcomes in Two Parallel Introductory Biochemistry Courses

    Science.gov (United States)

    Bevan, Samantha J.; Chan, Cecilia W. L.; Tanner, Julian A.

    2014-01-01

    Although there is increasing evidence for a relationship between courses that emphasize student engagement and achievement of student deep learning, there is a paucity of quantitative comparative studies in a biochemistry and molecular biology context. Here, we present a pedagogical study in two contrasting parallel biochemistry introductory…

  11. Design of a Comprehensive Biochemistry and Molecular Biology Experiment: Phase Variation Caused by Recombinational Regulation of Bacterial Gene Expression

    Science.gov (United States)

    Sheng, Xiumei; Xu, Shungao; Lu, Renyun; Isaac, Dadzie; Zhang, Xueyi; Zhang, Haifang; Wang, Huifang; Qiao, Zheng; Huang, Xinxiang

    2014-01-01

    Scientific experiments are indispensable parts of Biochemistry and Molecular Biology. In this study, a comprehensive Biochemistry and Molecular Biology experiment about "Salmonella enterica" serovar Typhi Flagellar phase variation has been designed. It consisted of three parts, namely, inducement of bacterial Flagellar phase variation,…

  12. Reactivity I: A Foundation-Level Course for Both Majors and Nonmajors in Integrated Organic, Inorganic, and Biochemistry

    Science.gov (United States)

    Schaller, Chris P.; Graham, Kate J.; Johnson, Brian J.; Jones, T. Nicholas; McIntee, Edward J.

    2015-01-01

    A foundation level course is presented that integrates aspects of organic, inorganic and biochemistry in the context of reactivity. The course was designed to serve majors in chemistry and other sciences (biochemistry, biology, nutrition), as well as nursing and pre-health professions students. Themes of the course were designed to highlight a…

  13. Guidelines for Educational Research in Biochemistry on Internet Sites

    Directory of Open Access Journals (Sweden)

    R.M. Lima

    2012-05-01

    Full Text Available The Internet has been used to support research in different areas, such as practical and educational  research  in  medical  and  biomedical  research.  There  are  several recommended  sites  for  carrying  biomedical  and  medical  research  (BERGER,  2003. Nevertheless,  few  studies  report  on  the  use  of  the  Internet  in  the  teaching  of Biochemistry. Considering the fact that there is no specific legislation for the use of the Internet  in  Brazil,  it  is  necessary  to  stimulate  self-regulation  of  the  sector  in  order  to establish  minimum  quality  standards,  safety,  and  reliability  of  sites  containing information  in  the  educational  area.  This  study  establishes  some  parameters  to  help guiding research for educational purposes on the internet. The following aspects should be  checked:  if  the  site  has  an  editorial  board  responsible  for  content  selection,  and whether  it  is  made  up  of  experts  in  the  area  of  expertise;  if  the  site  releases  updated scientific materials, and provides pedagogical content that fosters teaching and learning such  as  images  that  contribute  to  the  understanding  of  the  content,  educational software,  and  animation;  if  the  site  is  recommended  by  universities,  public  and  private qualified  institutions.  In  addition,  educational  sites  should  present  other  aspects, including transparency (regarding their educational purpose, quality (scientifically based information,  privacy  (related  to  the  user’s  personal  data,  responsibility  and  reliable sources.  Such  procedures  are  necessary  to  guarantee  that  searching  for  educational objectives will provide access to theoretical and pedagogical information quality.

  14. Semiconductor Electrical Measurements Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Semiconductor Electrical Measurements Laboratory is a research laboratory which complements the Optical Measurements Laboratory. The laboratory provides for Hall...

  15. Audio Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: Provides an environment and facilities for auditory display research. A primary focus is the performance use of binaurally rendered 3D sound in conjunction...

  16. Elastomers Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — Primary capabilities include: elastomer compounding in various sizes (micro, 3x5, 8x12, 8x15 rubber mills); elastomer curing and post curing (two 50-ton presses, one...

  17. A retrospective study of the clinical hematology and the serum biochemistry tests made on canine dirofilariasis cases in an animal hospital population in Bangkok, Thailand.

    Science.gov (United States)

    Niwetpathomwat, Anuchai; Kaewthamasorn, Morakot; Tiawsirisup, Sonthaya; Techangamsuwan, Somporn; Suvarnvibhaja, Siram

    2007-06-01

    Heartworm disease, caused by the filarial nematode, Dirofilaria immitis, is a major, potentially life-threatening disease of dogs, with worldwide distribution and global significance. It is not only of veterinary importance but it also has zoonotic potential in many regions. It is considered as an endemic disease in Thailand, although clinical data about the disease is rarely reported. The objectives of this study were to characterize the clinical hematology and the biochemistry of canine dirofilariasis cases admitted to Chulalongkorn University, Small Animal Teaching Hospital in Bangkok, Thailand, from 2001-2003. All hematology and serum biochemistry parameter interpretations were based on reference values. A total of 1023 dogs were evaluated in this study. Dogs were divided into three groups based on their heartworm classification (microfilaremic, occult and negative). The major hematological findings in microfilaremic dogs were a mild to moderate anemia, mild to severe thrombocytopenia, marked leukocytosis, moderate to marked neutrophilia, eosinophilia and monocytosis. The most common serum biochemical abnormalities in microfilaremic dogs included increased alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase with a mean+/-SD of 311+/-299, 82+/-76 and 50+/-38 IU/L, respectively. It is likely that this parasitic infection led to the impairment of the hematological and biochemical status of the infected dogs. We believe that investigation of these laboratory based parameters, when associated with the clinical signs, is a very important approach to be considered in the routine clinical follow up, as well as being important for therapeutic evaluations.

  18. A Learner-Centered Molecular Modeling Exercise for Allied Health Majors in a Biochemistry Class

    Science.gov (United States)

    Fletcher, Terace M.; Ershler, Jeff

    2014-01-01

    Learner-centered molecular modeling exercises in college science courses can be especially challenging for nonchemistry majors as students typically have a higher degree of anxiety and may not appreciate the relevance of the work. This article describes a learner-centered project given to allied health majors in a Biochemistry course. The project…

  19. A guide to using case-based learning in biochemistry education.

    Science.gov (United States)

    Kulak, Verena; Newton, Genevieve

    2014-01-01

    Studies indicate that the majority of students in undergraduate biochemistry take a surface approach to learning, associated with rote memorization of material, rather than a deep approach, which implies higher cognitive processing. This behavior relates to poorer outcomes, including impaired course performance and reduced knowledge retention. The use of case-based learning (CBL) into biochemistry teaching may facilitate deep learning by increasing student engagement and interest. Abundant literature on CBL exists but clear guidance on how to design and implement case studies is not readily available. This guide provides a representative review of CBL uses in science and describes the process of developing CBL modules to be used in biochemistry. Included is a framework to implement a directed CBL assisted with lectures in a content-driven biochemistry course regardless of class size. Moreover, this guide can facilitate adopting CBL to other courses. Consequently, the information presented herein will be of value to undergraduate science educators with an interest in active learning pedagogies.

  20. The Concept Lens Diagram: A New Mechanism for Presenting Biochemistry Content in Terms of "Big Ideas"

    Science.gov (United States)

    Rowland, Susan L.; Smith, Christopher A.; Gillam, Elizabeth M. A.; Wright, Tony

    2011-01-01

    A strong, recent movement in tertiary education is the development of conceptual, or "big idea" teaching. The emphasis in course design is now on promoting key understandings, core competencies, and an understanding of connections between different fields. In biochemistry teaching, this radical shift from the content-based tradition is being…

  1. What Skills Should Students of Undergraduate Biochemistry and Molecular Biology Programs Have upon Graduation?

    Science.gov (United States)

    White, Harold B.; Benore, Marilee A.; Sumter, Takita F.; Caldwell, Benjamin D.; Bell, Ellis

    2013-01-01

    Biochemistry and molecular biology (BMB) students should demonstrate proficiency in the foundational concepts of the discipline and possess the skills needed to practice as professionals. To ascertain the skills that should be required, groups of BMB educators met in several focused workshops to discuss the expectations with the ultimate goal of…

  2. Biochemistry - Open TG-GATEs | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...y.zip File URL: ftp://ftp.biosciencedbc.jp/archive/open-tggates/LATEST/open_tggat...e Database Description Download License Update History of This Database Site Policy | Contact Us Biochemistry - Open TG-GATEs | LSDB Archive ...

  3. Biochemistry, proteomics, and phosphoproteomics of plant mitochondria from non-photosynthetic cells

    DEFF Research Database (Denmark)

    Havelund, Jesper; Thelen, Jay J.; Møller, Ian Max

    2013-01-01

    functions depending on the tissue and cell type, as well as environmental conditions. We will here review the biochemistry and proteomics of mitochondria from non-green cells and organs, which differ from those of photosynthetic organs in a number of respects. We will briefly cover purification...

  4. Improving Student Understanding of Lipids Concepts in a Biochemistry Course Using Test-Enhanced Learning

    Science.gov (United States)

    Horn, Savannah; Hernick, Marcy

    2015-01-01

    Test-enhanced learning has successfully been used as a means to enhance learning and promote knowledge retention in students. We have examined whether this approach could be used in a biochemistry course to enhance student learning about lipids-related concepts. Students were provided access to two optional learning modules with questions related…

  5. The Metabolic Effects of Low-Carbohydrate Diets and Incorporation into a Biochemistry Course

    Science.gov (United States)

    Pogozelski, Wendy; Arpaia, Nicholas; Priore, Salvatore

    2005-01-01

    One of the challenges in teaching biochemistry is facilitating students' interest in and mastery of metabolism. The many pathways and modes of regulation can be overwhelming for students to learn and difficult for professors to teach in an engaging manner. We have found it useful to take advantage of prevailing interest in popular yet…

  6. Book Review: "The Rhizosphere: Biochemistry and Organic Substances at the Soil-Plant Interface, Second Edition"

    Science.gov (United States)

    The complexity of the biological, chemical, and physical interactions occurring in the volume of soil surrounding the root of a growing plant dictates that a multidisciplinary approach must be taken to improve our understanding of this rhizosphere. Hence, "The Rhizosphere: Biochemistry and Organic S...

  7. A Statistical Analysis of College Biochemistry Textbooks in China: The Statuses on the Publishing and Usage

    Science.gov (United States)

    Zhou, Ping; Wang, Qinwen; Yang, Jie; Li, Jingqiu; Guo, Junming; Gong, Zhaohui

    2015-01-01

    This study aimed to investigate the statuses on the publishing and usage of college biochemistry textbooks in China. A textbook database was constructed and the statistical analysis was adopted to evaluate the textbooks. The results showed that there were 945 (~57%) books for theory teaching, 379 (~23%) books for experiment teaching and 331 (~20%)…

  8. Essential Concepts and Underlying Theories from Physics, Chemistry, and Mathematics for "Biochemistry and Molecular Biology" Majors

    Science.gov (United States)

    Wright, Ann; Provost, Joseph; Roecklein-Canfield, Jennifer A.; Bell, Ellis

    2013-01-01

    Over the past two years, through an NSF RCN UBE grant, the ASBMB has held regional workshops for faculty members from around the country. The workshops have focused on developing lists of Core Principles or Foundational Concepts in Biochemistry and Molecular Biology, a list of foundational skills, and foundational concepts from Physics, Chemistry,…

  9. Impact of an In-Class Biochemistry Mini-Conference on Students' Perception of Science

    Science.gov (United States)

    Gerczei, Timea

    2016-01-01

    The work presented here is the summary of a 3 year study that aimed to uncover how students' perception of science changes with the chance to participate in a mini-conference that is incorporated into the biochemistry lecture course. Students were asked to work in groups of 2 or 3 and research a topic that is related to the material covered in…

  10. Using Adobe Flash Animations of Electron Transport Chain to Teach and Learn Biochemistry

    Science.gov (United States)

    Teplá, Milada; Klímová, Helena

    2015-01-01

    Teaching the subject of the electron transport chain is one of the most challenging aspects of the chemistry curriculum at the high school level. This article presents an educational program called "Electron Transport Chain" which consists of 14 visual animations including a biochemistry quiz. The program was created in the Adobe Flash…

  11. Foundational Concepts and Underlying Theories for Majors in "Biochemistry and Molecular Biology"

    Science.gov (United States)

    Tansey, John T.; Baird, Teaster, Jr.; Cox, Michael M.; Fox, Kristin M.; Knight, Jennifer; Sears, Duane; Bell, Ellis

    2013-01-01

    Over the past two years, through an NSF RCN UBE grant, the ASBMB has held regional workshops for faculty members and science educators from around the country that focused on identifying: 1) core principles of biochemistry and molecular biology, 2) essential concepts and underlying theories from physics, chemistry, and mathematics, and 3)…

  12. Motivating Active Learning of Biochemistry through Artistic Representation of Scientific Concepts

    Science.gov (United States)

    Bruna, Carola

    2013-01-01

    First-year students often feel discouraged, especially with courses that require complex thinking and involve establishing relations between different subjects such as biochemistry. It has been proposed that student-centred pedagogy can achieve motivation and improve learning. In this context, this case study reports the use of art as a strategy…

  13. Implementing an Active Learning Environment to Influence Students' Motivation in Biochemistry

    Science.gov (United States)

    Cicuto, Camila Aparecida Tolentino; Torres, Bayardo Baptista

    2016-01-01

    The Biochemistry: Biomolecules Structure and Metabolism course's goal is to promote meaningful learning through an active learning environment. Thus, study periods (SP) and discussion groups (DG) are used as a substitute for lecture classes. The goal of this study was to evaluate how this learning environment influences students' motivation (n =…

  14. Teaching of Biochemistry in Medical School: A Well-Trodden Pathway?

    Science.gov (United States)

    Mathews, Michael B.; Stagnaro-Green, Alex

    2008-01-01

    Biochemistry and molecular biology occupy a unique place in the medical school curriculum. They are frequently studied prior to medical school and are fundamental to the teaching of biomedical sciences in undergraduate medical education. These two circumstances, and the trend toward increased integration among the disciplines, have led to…

  15. The Concept Lens Diagram: A New Mechanism for Presenting Biochemistry Content in Terms of "Big Ideas"

    Science.gov (United States)

    Rowland, Susan L.; Smith, Christopher A.; Gillam, Elizabeth M. A.; Wright, Tony

    2011-01-01

    A strong, recent movement in tertiary education is the development of conceptual, or "big idea" teaching. The emphasis in course design is now on promoting key understandings, core competencies, and an understanding of connections between different fields. In biochemistry teaching, this radical shift from the content-based tradition is…

  16. The Views of Undergraduates about Problem-Based Learning Applications in a Biochemistry Course

    Science.gov (United States)

    Tarhan, Leman; Ayyildiz, Yildizay

    2015-01-01

    The effect of problem-based learning (PBL) applications in an undergraduate biochemistry course on students' interest in this course was investigated through four modules during one semester. Students' views about active learning and improvement in social skills were also collected and evaluated. We conducted the study with 36 senior students from…

  17. A Guide to Using Case-Based Learning in Biochemistry Education

    Science.gov (United States)

    Kulak, Verena; Newton, Genevieve

    2014-01-01

    Studies indicate that the majority of students in undergraduate biochemistry take a surface approach to learning, associated with rote memorization of material, rather than a deep approach, which implies higher cognitive processing. This behavior relates to poorer outcomes, including impaired course performance and reduced knowledge retention. The…

  18. [Recent progress on analytical chemistry and biochemistry of D-amino acids].

    Science.gov (United States)

    Imai, K; Kato, M; Huang, Y; Ichihara, H; Fukushima, T; Santa, T; Homma, H

    1997-11-01

    Recent findings that D-amino acids, especially D-aspartic acid and D-serine, exist in vivo in the mammalian tissues (brain and peripheries), prompted us now to investigate their biological and pathological roles in mammals. In this review, the overview of the progress of analytical chemistry and biochemistry of D-amino acids is described.

  19. Students' Preferred Teaching Techniques for Biochemistry in Biomedicine and Medicine Courses

    Science.gov (United States)

    Novelli, Ethel L.B.; Fernandes, Ana Angelica H.

    2007-01-01

    The aim of this study was to investigate the students' preferred teaching techniques, such as traditional blackboard, power-point, or slide-projection, for biochemistry discipline in biomedicine and medicine courses from Sao Paulo State University, UNESP, Botucatu, Sao Paulo, Brazil. Preferences for specific topic and teaching techniques were…

  20. Teaching foundational topics and scientific skills in biochemistry within the conceptual framework of HIV protease.

    Science.gov (United States)

    Johnson, R Jeremy

    2014-01-01

    HIV protease has served as a model protein for understanding protein structure, enzyme kinetics, structure-based drug design, and protein evolution. Inhibitors of HIV protease are also an essential part of effective HIV/AIDS treatment and have provided great societal benefits. The broad applications for HIV protease and its inhibitors make it a perfect framework for integrating foundational topics in biochemistry around a big picture scientific and societal issue. Herein, I describe a series of classroom exercises that integrate foundational topics in biochemistry around the structure, biology, and therapeutic inhibition of HIV protease. These exercises center on foundational topics in biochemistry including thermodynamics, acid/base properties, protein structure, ligand binding, and enzymatic catalysis. The exercises also incorporate regular student practice of scientific skills including analysis of primary literature, evaluation of scientific data, and presentation of technical scientific arguments. Through the exercises, students also gain experience accessing computational biochemical resources such as the protein data bank, Proteopedia, and protein visualization software. As these HIV centered exercises cover foundational topics common to all first semester biochemistry courses, these exercises should appeal to a broad audience of undergraduate students and should be readily integrated into a variety of teaching styles and classroom sizes.

  1. Filtrates & Residues: Hemoglobinometry--A Biochemistry Experiment that Utilizes the Principles of Transition Metal Chemistry.

    Science.gov (United States)

    Giuliano, Vincenzo; Rieck, John Paul

    1987-01-01

    Describes a chemistry experiment dealing with hemoglobinometry that can apply to transition metal chemistry, colorimetry, and biochemistry. Provides a detailed description of the experimental procedure, including discussions of the preparation of the cyanide reagent, colorimetric measurements, and waste disposal and treatment. (TW)

  2. Sustaining Change in Upper Level Courses: Peer-Led Workshops in Organic Chemistry and Biochemistry

    Science.gov (United States)

    Platt, Terry; Roth, Vicki; Kampmeier, Jack A.

    2008-01-01

    Our peer-led collaborative learning groups, called Workshops, have now had extended success in two upper-level courses in chemistry and biochemistry. These Workshops are in turn supported by a third upper-level course for training peer-leaders. Our data confirm that the initial positive results from the introduction of Workshops in organic…

  3. Need Assessment of Enhancing the Weightage of Applied Biochemistry in the Undergraduate Curriculum at MGIMS, Sevagram

    Science.gov (United States)

    Kumar, Satish; Jena, Lingaraja; Vagha, Jayant

    2016-01-01

    In order to review the need assessment of enhancing the weightage of Applied Biochemistry in the undergraduate curriculum at Mahatma Gandhi Institute of Medical Sciences (MGIMS), Sevagram, a validated questionnaire was sent to 453 participants which include 387 undergraduate students, 11 interns, 23 postgraduate students, and 32 faculty members. A…

  4. Reversible Ligand Binding Reactions: Why Do Biochemistry Students Have Trouble Connecting the Dots?

    Science.gov (United States)

    Sears, Duane W.; Thompson, Scott E.; Saxon, S. Robin

    2007-01-01

    Adaptive chemical behavior is essential for an organism's function and survival, and it is no surprise that biological systems are capable of responding both rapidly and selectively to chemical changes in the environment. To elucidate an organism's biochemistry, its chemical reactions need to be characterized in ways that reflect the normal…

  5. Integrative Metabolism: An Interactive Learning Tool for Nutrition, Biochemistry, and Physiology

    Science.gov (United States)

    Carey, Gale

    2010-01-01

    Metabolism is a dynamic, simultaneous, and integrative science that cuts across nutrition, biochemistry, and physiology. Teaching this science can be a challenge. The use of a scenario-based, visually appealing, interactive, computer-animated CD may overcome the limitations of learning "one pathway at a time" and engage two- and…

  6. Gold nanoparticles: preparation, functionalisation and applications in biochemistry and immunochemistry

    Energy Technology Data Exchange (ETDEWEB)

    Dykman, Lev A; Bogatyrev, Vladimir A [Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (Russian Federation)

    2007-02-28

    The review summarises data on the synthesis and functionalisation of gold nanoparticles and their applications in biological investigations. Particular attention is given to applications of colloidal gold in solid-phase assays, immunoassay and studies of biologically active compounds by vibrational spectroscopy. A special section deals with the use of gold nanoparticles as antigen carriers in immunisation.

  7. Reference intervals for selected serum biochemistry analytes in cheetahs Acinonyx jubatus.

    Science.gov (United States)

    Hudson-Lamb, Gavin C; Schoeman, Johan P; Hooijberg, Emma H; Heinrich, Sonja K; Tordiffe, Adrian S W

    2016-02-26

    Published haematologic and serum biochemistry reference intervals are very scarce for captive cheetahs and even more for free-ranging cheetahs. The current study was performed to establish reference intervals for selected serum biochemistry analytes in cheetahs. Baseline serum biochemistry analytes were analysed from 66 healthy Namibian cheetahs. Samples were collected from 30 captive cheetahs at the AfriCat Foundation and 36 free-ranging cheetahs from central Namibia. The effects of captivity-status, age, sex and haemolysis score on the tested serum analytes were investigated. The biochemistry analytes that were measured were sodium, potassium, magnesium, chloride, urea and creatinine. The 90% confidence interval of the reference limits was obtained using the non-parametric bootstrap method. Reference intervals were preferentially determined by the non-parametric method and were as follows: sodium (128 mmol/L - 166 mmol/L), potassium (3.9 mmol/L - 5.2 mmol/L), magnesium (0.8 mmol/L - 1.2 mmol/L), chloride (97 mmol/L - 130 mmol/L), urea (8.2 mmol/L - 25.1 mmol/L) and creatinine (88 µmol/L - 288 µmol/L). Reference intervals from the current study were compared with International Species Information System values for cheetahs and found to be narrower. Moreover, age, sex and haemolysis score had no significant effect on the serum analytes in this study. Separate reference intervals for captive and free-ranging cheetahs were also determined. Captive cheetahs had higher urea values, most likely due to dietary factors. This study is the first to establish reference intervals for serum biochemistry analytes in cheetahs according to international guidelines. These results can be used for future health and disease assessments in both captive and free-ranging cheetahs.

  8. Reference intervals for selected serum biochemistry analytes in cheetahs (Acinonyx jubatus).

    Science.gov (United States)

    Hudson-Lamb, Gavin C; Schoeman, Johan P; Hooijberg, Emma H; Heinrich, Sonja K; Tordiffe, Adrian S W

    2016-02-26

    Published haematologic and serum biochemistry reference intervals are very scarce for captive cheetahs and even more for free-ranging cheetahs. The current study was performed to establish reference intervals for selected serum biochemistry analytes in cheetahs. Baseline serum biochemistry analytes were analysed from 66 healthy Namibian cheetahs. Samples were collected from 30 captive cheetahs at the AfriCat Foundation and 36 free-ranging cheetahs from central Namibia. The effects of captivity-status, age, sex and haemolysis score on the tested serum analytes were investigated. The biochemistry analytes that were measured were sodium, potassium, magnesium, chloride, urea and creatinine. The 90% confidence interval of the reference limits was obtained using the non-parametric bootstrap method. Reference intervals were preferentially determined by the non-parametric method and were as follows: sodium (128 mmol/L - 166 mmol/L), potassium (3.9 mmol/L - 5.2 mmol/L), magnesium (0.8 mmol/L - 1.2 mmol/L), chloride (97 mmol/L - 130 mmol/L), urea (8.2 mmol/L - 25.1 mmol/L) and creatinine (88 µmol/L - 288 µmol/L). Reference intervals from the current study were compared with International Species Information System values for cheetahs and found to be narrower. Moreover, age, sex and haemolysis score had no significant effect on the serum analytes in this study. Separate reference intervals for captive and free-ranging cheetahs were also determined. Captive cheetahs had higher urea values, most likely due to dietary factors. This study is the first to establish reference intervals for serum biochemistry analytes in cheetahs according to international guidelines. These results can be used for future health and disease assessments in both captive and free-ranging cheetahs.

  9. Reference intervals for selected serum biochemistry analytes in cheetahs (Acinonyx jubatus

    Directory of Open Access Journals (Sweden)

    Gavin C. Hudson-Lamb

    2016-02-01

    Full Text Available Published haematologic and serum biochemistry reference intervals are very scarce for captive cheetahs and even more for free-ranging cheetahs. The current study was performed to establish reference intervals for selected serum biochemistry analytes in cheetahs. Baseline serum biochemistry analytes were analysed from 66 healthy Namibian cheetahs. Samples were collected from 30 captive cheetahs at the AfriCat Foundation and 36 free-ranging cheetahs from central Namibia. The effects of captivity-status, age, sex and haemolysis score on the tested serum analytes were investigated. The biochemistry analytes that were measured were sodium, potassium, magnesium, chloride, urea and creatinine. The 90% confidence interval of the reference limits was obtained using the non-parametric bootstrap method. Reference intervals were preferentially determined by the non-parametric method and were as follows: sodium (128 mmol/L – 166 mmol/L, potassium (3.9 mmol/L – 5.2 mmol/L, magnesium (0.8 mmol/L – 1.2 mmol/L, chloride (97 mmol/L – 130 mmol/L, urea (8.2 mmol/L – 25.1 mmol/L and creatinine (88 µmol/L – 288 µmol/L. Reference intervals from the current study were compared with International Species Information System values for cheetahs and found to be narrower. Moreover, age, sex and haemolysis score had no significant effect on the serum analytes in this study. Separate reference intervals for captive and free-ranging cheetahs were also determined. Captive cheetahs had higher urea values, most likely due to dietary factors. This study is the first to establish reference intervals for serum biochemistry analytes in cheetahs according to international guidelines. These results can be used for future health and disease assessments in both captive and free-ranging cheetahs.

  10. Biochemistry on the game board: improvements in the teaching-learning process in basic education

    Directory of Open Access Journals (Sweden)

    F. A. Silva

    2015-08-01

    Full Text Available According to the Curriculum Guidelines of Biology of Basic Education "school should encourage the pedagogical practice based on different methodologies, valuing the teaching concepts, the learning (internalization and the evaluation that allow teachers and students being aware of the need for emancipatory transformation”.  The teaching of biochemistry and its contents in basic education are not discussed as a structuring content within the Biology subject, but are included in related content such as cellular biology. The objective of this study was to inventory the contents and methodologies related to biochemistry taught by teachers of basic education and produce teaching-learning materials as contribution to these contents from the inventory results. The methodology was developed from the analysis of the questionnaires applied to biology teachers from public schools of Guarapuava-Pr. The results showed that the teaching of biochemistry and biology discipline, although it comes from the same area, are designed as two unattached areas, meaning there is no perception by teachers in relation to the implicit conceptual intercept in teaching Biology and Biochemistry. To this end, the profile of the game Grow was adapted using themes related to Biochemistry. The game consists of 45 cards and a game board with a track. The player who gives more write answers to the questions reaches the end and wins the match. For game evaluation participants answered a questionnaire at the end of the activity. Most of the participants argued that the process has contributed to ensure the assimilation of the contents, since it is a leisure activity with effective participation of students. Thus, the obtained data confirmed the assumption of  Pedroso (2009, which states that the games allow a significant teacher-student interaction, disseminating scientific knowledge from the views and experiences of the student.

  11. Enhancing Predictive Accuracy of Cardiac Autonomic Neuropathy Using Blood Biochemistry Features and Iterative Multitier Ensembles.

    Science.gov (United States)

    Abawajy, Jemal; Kelarev, Andrei; Chowdhury, Morshed U; Jelinek, Herbert F

    2016-01-01

    Blood biochemistry attributes form an important class of tests, routinely collected several times per year for many patients with diabetes. The objective of this study is to investigate the role of blood biochemistry for improving the predictive accuracy of the diagnosis of cardiac autonomic neuropathy (CAN) progression. Blood biochemistry contributes to CAN, and so it is a causative factor that can provide additional power for the diagnosis of CAN especially in the absence of a complete set of Ewing tests. We introduce automated iterative multitier ensembles (AIME) and investigate their performance in comparison to base classifiers and standard ensemble classifiers for blood biochemistry attributes. AIME incorporate diverse ensembles into several tiers simultaneously and combine them into one automatically generated integrated system so that one ensemble acts as an integral part of another ensemble. We carried out extensive experimental analysis using large datasets from the diabetes screening research initiative (DiScRi) project. The results of our experiments show that several blood biochemistry attributes can be used to supplement the Ewing battery for the detection of CAN in situations where one or more of the Ewing tests cannot be completed because of the individual difficulties faced by each patient in performing the tests. The results show that AIME provide higher accuracy as a multitier CAN classification paradigm. The best predictive accuracy of 99.57% has been obtained by the AIME combining decorate on top tier with bagging on middle tier based on random forest. Practitioners can use these findings to increase the accuracy of CAN diagnosis.

  12. Evaluation of Student-made Blogs in Basicand Advanced Biochemistry Classes

    Directory of Open Access Journals (Sweden)

    E. Cubas

    2011-04-01

    Full Text Available Preliminary results of the experience of student-made clinical biochemistry blogs were reported at SBBq-2010 (abstract K-5. Herein, five teaching-semesters and the opinion of former students were evaluated. Since the teaching-semester of 2008-1, Basic Biochemistry (BioBio students should prepare blog-assignments on clinical issues. Students' acceptance was evaluated through 6-point Likert-type questionnaires. Positive responses were those marking 4 to 6. A total of 348 BioBio students from five teaching-semesters answered the questionnaire; 77% of them agreed that preparing blogs was enjoyable, having a positive effect on their formation. Moreover, 81% of students agreed that BioBio blogs are relevant learning tools and 78% believedthat BioBio blogs boosted interest for biochemistry. Moreover, students' acceptance 1 year after taking BioBio was evaluated. Students (n=50 were dividedin (i those who had attended BioBio only, and (ii those who had also taken Advanced Biochemistry, together with blog tutoring. In the first group, 72% agreed that the information acquired during blog elaboration was useful atthe time of interview; 76% judged that blog elaboration boosted interest for the discipline. For thosein the second group evaluations were 100% and 82%, respectively. Results show maintenance of acceptance over 1 year and effective interest for blog-assignment for thosein basic and advanced biochemistry classes.

  13. Correlation of Preadmission Organic Chemistry Courses and Academic Performance in Biochemistry at a Midwest Chiropractic Doctoral Program*

    Science.gov (United States)

    McRae, Marc P.

    2010-01-01

    Purpose: Organic chemistry has been shown to correlate with academic success in the preclinical years of medicine, dentistry, and graduate physiology. The purpose of this study is to examine the relationship between undergraduate organic chemistry grades and first-semester biochemistry grades at a Midwest chiropractic doctoral program. Methods: Students enrolled in a first-semester biochemistry course who had completed the prerequisite courses in organic chemistry offered at this same institution were entered into the study. The total grade for each of the three courses was calculated using the midterm and final exam raw scores with a weighting of 50% each. Analysis consisted of obtaining correlation coefficients between the total grades of organic 1 with biochemistry and organic 2 with biochemistry. Using the biochemistry total grade, the students were divided into quartiles and course grades for both organic chemistry 1 and 2 were calculated. Results: For the 109 students in the study, the correlation coefficient between the biochemistry and organic chemistry 1 and biochemistry and organic chemistry 2 courses was r = 0.744 and r = 0.725, respectively. The difference in organic chemistry grades between those in the first and fourth quartiles was 63.2% and 86.9% for organic chemistry 1 (p < .001) and 60.9% and 79.4% for organic chemistry 2 (p < .001). Conclusion: This study shows that organic chemistry can be used as an indicator of future academic success in a chiropractic biochemistry course. Knowledge of such a relationship could prove useful to identify students who may potentially run into academic difficulty with first-year biochemistry PMID:20480012

  14. A historical perspective on radioisotopic tracers in metabolism and biochemistry.

    Science.gov (United States)

    Lappin, Graham

    2015-01-01

    Radioisotopes are used routinely in the modern laboratory to trace and quantify a myriad of biochemical processes. The technique has a captivating history peppered with groundbreaking science and with more than its share of Nobel Prizes. The discovery of radioactivity at the end of the 19th century paved the way to understanding atomic structure and quickly led to the use of radioisotopes to trace the fate of molecules as they flowed through complex organic life. The 1940s saw the first radiotracer studies using homemade instrumentation and analytical techniques such as paper chromatography. This article follows the history of radioisotopic tracers from meager beginnings, through to the most recent applications. The author hopes that those researchers involved in radioisotopic tracer studies today will pause to remember the origins of the technique and those who pioneered this fascinating science.

  15. Hanford cultural resources laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Wright, M.K.

    1995-06-01

    This section of the 1994 Hanford Site Environmental Report describes activities of the Hanford Cultural Resources Laboratory (HCRL) which was established by the Richland Operations Office in 1987 as part of PNL.The HCRL provides support for the management of the archaeological, historical, and traditional cultural resources of the site in a manner consistent with the National Historic Preservation Act, the Native American Graves Protection and Repatriation Act, and the American Indian Religious Freedom Act.

  16. 42 CFR 493.1403 - Condition: Laboratories performing moderate complexity testing; laboratory director.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Condition: Laboratories performing moderate complexity testing; laboratory director. 493.1403 Section 493.1403 Public Health CENTERS FOR MEDICARE... LABORATORY REQUIREMENTS Personnel for Nonwaived Testing Laboratories Performing Moderate Complexity...

  17. Saxton Transportation Operations Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Saxton Transportation Operations Laboratory (Saxton Laboratory) is a state-of-the-art facility for conducting transportation operations research. The laboratory...

  18. METABOLIC WAR: A VARIATION FOR METABOLIC BIOCHEMISTRY LEARNING OF A WORLDLY KNOWN BOARD GAME

    Directory of Open Access Journals (Sweden)

    C. M. Anjos

    2008-05-01

    Full Text Available Biomedical careers are highly wished by young students in Brazil. Although future jobs,  academic knowledge and higher earnings  are tempting reasons for this life choice, few of them are aware  of  the difficult path through the  basic classes. Advanced and specific disciplines  are easier to associate with the professional career itself, but few students can identify the importance  of the basic knowledge for their future work. Biochemistry is one of the most difficult  disciplines  for Brazilian students, probably due to the level of abstraction needed to fully learn and understand the topics. Some recent experimental tools, such as bioinformatics, are now helping students with the learning process, providing visual data for understanding biomolecule structure.  In addition to this, biochemical reactions  could be even tougher because of the many variables involved.  To facilitate the learning process for metabolic biochemistry, we created a game based on the board game WAR®,  using Photoshop software. Named Metabolic War, it keeps the same basic rules of WAR®, but with some minor changes. The continents are metabolic pathways (citric acid cycle, glycolysis, beta-oxidation, etc and the countries are metabolic intermediates. Similarly to the original game, players must conquer an objective (one or more metabolic pathways by dominating intermediates. But the desired intermediate must be a possible product from an intermediate the player already owns. This  and other  games were produced by Biomedicine  undergraduate  students  in Metabolic Biochemistry classes. It was presented to other students, who tested and acknowledged it as a great help in understanding metabolic biochemistry,  giving a great understanding of integrative metabolism. Keywords: game; Biochemistry; Metabolic Biochemistry learning; science learning; playful learning.

  19. Teaching Biochemistry Using New Strategies in the Universidad de Concepción (Symposium

    Directory of Open Access Journals (Sweden)

    José Martínez Oyanedel

    2012-05-01

    Full Text Available The new paradigms of higher education require new teaching strategies to meet the  learning  objectives  of  Biochemistry  courses.  In  our  department  there  have been some early educational innovations through the incorporation of topics of protein  structure  in  web  page,  which  then  evolved  into  interactive  java applications  based  on  the  use  of  CHIME,  and  is  currently  being  adapted  to Jmol.  Another  tool  used  is  the  PROTLAB  software,  which  is  a  simulator  of protein  purification  with  40  different  proteins.  Recently  have  added  new activities to motivate the study of Biochemistry, especially first years student: Art and  Biochemistry  where  the  students  express  some  of  the  biochemistry concepts  through  artistic  expression  and  are  graded  including  students.  For senior  student,  have  been  designed  activities  to  apply  the  basic  knowledge  of biochemistry  to  the  explanation  of  everyday  events  for  the  general  public, through the generation of a Wiki in a simple language. Debates activities have also  been  established  for  discussion  of  topics  related  to  biochemistry.  Also applications available in the web are used to reinforce some topics. All activities have  a  greater  acceptance  of  students,  although  the  effect  on  learning outcomes is ambiguous

  20. Lunar laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Keaton, P.W.; Duke, M.B.

    1986-01-01

    An international research laboratory can be established on the Moon in the early years of the 21st Century. It can be built using the transportation system now envisioned by NASA, which includes a space station for Earth orbital logistics and orbital transfer vehicles for Earth-Moon transportation. A scientific laboratory on the Moon would permit extended surface and subsurface geological exploration; long-duration experiments defining the lunar environment and its modification by surface activity; new classes of observations in astronomy; space plasma and fundamental physics experiments; and lunar resource development. The discovery of a lunar source for propellants may reduce the cost of constructing large permanent facilities in space and enhance other space programs such as Mars exploration. 29 refs.

  1. Virtual Laboratories

    CERN Document Server

    Hut, P

    2006-01-01

    At the frontier of most areas in science, computer simulations play a central role. The traditional division of natural science into experimental and theoretical investigations is now completely outdated. Instead, theory, simulation, and experimentation form three equally essential aspects, each with its own unique flavor and challenges. Yet, education in computational science is still lagging far behind, and the number of text books in this area is minuscule compared to the many text books on theoretical and experimental science. As a result, many researchers still carry out simulations in a haphazard way, without properly setting up the computational equivalent of a well equipped laboratory. The art of creating such a virtual laboratory, while providing proper extensibility and documentation, is still in its infancy. A new approach is described here, Open Knowledge, as an extension of the notion of Open Source software. Besides open source code, manuals, and primers, an open knowledge project provides simul...

  2. Vegetation Biochemistry: What Can Imaging Spectrometry Tell Us About Canopies?

    Science.gov (United States)

    Goetz, Alexander F. H.; Gao, Bo-Cai; Wessman, Carol

    1991-01-01

    Changes in ecosystem processes such as productivity and decomposition may be expressed in the canopy foliar chemistry resulting from altered carbon allocation patterns, metabolic processes and nutrient availability. Understanding carbon balance on land over large regions requires quantitative determination of leaf constituents such as lignin and total nitrogen from remote sensing imaging systems. Results from spectral reflectance measurements of stacked leaves in the laboratory show that spectrum matching techniques are applicable to the derivation of the equivalent liquid water thickness in plants as well as to the extraction of dry leaf matter reflectance spectra from spectra of green leaves. The residual spectra derived by subtracting water spectra from the spectra of green leaves shows a feature at 1.72 micrometers that can be related to the lignin content of the leaves. Oak leaves have a deeper residual absorption feature than do cotton leaves which is consistent with their relative lignin content. Similar results are achieved when deriving the residuals from images taken over areas of grass and pine trees. Imaging spectrometry provides promise in developing images of various foliar biochemical constituents.

  3. [Estimation of uncertainty of measurement in clinical biochemistry].

    Science.gov (United States)

    Enea, Maria; Hristodorescu, Cristina; Schiriac, Corina; Morariu, Dana; Mutiu, Tr; Dumitriu, Irina; Gurzu, B

    2009-01-01

    The uncertainty of measurement (UM) or measurement uncertainty is known as the parameter associated with the result of a measurement. Repeated measurements usually reveal slightly different results for the same analyte, sometimes a little higher, sometimes a little lower, because the results of a measurement are depending not only by the analyte itself, but also, by a number of error factors that could give doubts about the estimate. The uncertainty of the measurement represent the quantitative, mathematically expression of this doubt. UM is a range of measured values which is probably to enclose the true value of the measured. Calculation of UM for all types of laboratories is regularized by the ISO Guide to the Expression of Uncertainty in Measurement (abbreviated GUM) and the SR ENV 13005 : 2003 (both recognized by European Accreditation). Even if the GUM rules about UM estimation are very strictly, the offering of the result together with UM will increase the confidence of customers (patients or physicians). In this study the authors are presenting the possibilities of UM assessing in labs from our country by using the data obtained in the procedures of methods validation, during the internal and external quality control.

  4. Haematology and Plasma Biochemistry of Wild Black Flying-Foxes, (Pteropus alecto in Queensland, Australia.

    Directory of Open Access Journals (Sweden)

    Lee McMichael

    Full Text Available This paper establishes reference ranges for hematologic and plasma biochemistry values in wild Black flying-foxes (Pteropus alecto captured in South East Queensland, Australia. Values were found to be consistent with those of other Pteropus species. Four hundred and forty-seven animals were sampled over 12 months and significant differences were found between age, sex, reproductive and body condition cohorts in the sample population. Mean values for each cohort fell within the determined normal adult reference range, with the exception of elevated levels of alkaline phosphatase in juvenile animals. Hematologic and biochemistry parameters of injured animals showed little or no deviation from the normal reference values for minor injuries, while two animals with more severe injury or abscessation showed leucocytosis, anaemia, thrombocytosis, hyperglobulinemia and hypoalbuminemia.

  5. Haematology and Plasma Biochemistry of Wild Black Flying-Foxes, (Pteropus alecto) in Queensland, Australia

    Science.gov (United States)

    McMichael, Lee; Edson, Daniel; McLaughlin, Amanda; Mayer, David; Kopp, Steven; Meers, Joanne; Field, Hume

    2015-01-01

    This paper establishes reference ranges for hematologic and plasma biochemistry values in wild Black flying-foxes (Pteropus alecto) captured in South East Queensland, Australia. Values were found to be consistent with those of other Pteropus species. Four hundred and forty-seven animals were sampled over 12 months and significant differences were found between age, sex, reproductive and body condition cohorts in the sample population. Mean values for each cohort fell within the determined normal adult reference range, with the exception of elevated levels of alkaline phosphatase in juvenile animals. Hematologic and biochemistry parameters of injured animals showed little or no deviation from the normal reference values for minor injuries, while two animals with more severe injury or abscessation showed leucocytosis, anaemia, thrombocytosis, hyperglobulinemia and hypoalbuminemia. PMID:25938493

  6. Promoting active learning of graduate student by deep reading in biochemistry and microbiology pharmacy curriculum.

    Science.gov (United States)

    Peng, Ren

    2017-01-06

    To promote graduate students' active learning, deep reading of high quality papers was done by graduate students enrolled in biochemistry and microbiology pharmacy curriculum offered by college of life science, Jiangxi Normal University from 2013 to 2015. The number of graduate students, who participated in the course in 2013, 2014, and 2015 were eleven, thirteen and fifteen, respectively. Through deep reading of papers, presentation, and group discussion in the lecture, these graduate students have improved their academic performances effectively, such as literature search, PPT document production, presentation management, specialty document reading, academic inquiry, and analytical and comprehensive ability. The graduate students also have increased their understanding level of frontier research, scientific research methods, and experimental methods. © 2017 by The International Union of Biochemistry and Molecular Biology, 2017.

  7. Hematology and plasma biochemistry reference range values for free-ranging desert tortoises in Arizona.

    Science.gov (United States)

    Dickinson, Vanessa M; Jarchow, James L; Trueblood, Mark H

    2002-01-01

    Baseline values and ranges for 10 hematologic and 32 plasma chemistry parameters were analyzed for 36 free-ranging Sonoran desert tortoises (Gopherus agassizzi) collected in Yavapai and La Paz Counties (Arizona, USA) from 1990 to 1995. Tortoises were radio tagged from 1990 to 1994, and attempts were made to recapture them three times a year. Tortoises were weighed, measured, and chemically immobilized to collect blood for hematology and blood chemistry assessments. Tortoise biochemistry differed (P < 0.01) between sites and sexes and among seasons and years. Normal reference ranges for hematologic and plasma biochemistry parameters were determined. Seasonal and annual differences in hematology and blood chemistry were related to rainfall patterns, forage availability, and physiological condition.

  8. Summary report of a workshop on research opportunities in plant biochemistry, December 11--13, 1992, Kona, Hawaii

    Energy Technology Data Exchange (ETDEWEB)

    1992-12-31

    A DOE-sponsored workshop was held December 11--13, 1992 in Kona, Hawaii to discuss those aspects of fundamental research in plant biochemistry deemed essential to provide the basic information base necessary for exploiting plant biotechnology to meet future societal needs. Twenty nine scientists, with interests representing many of the various areas of plant biochemistry, participated. The workshop was intended to define in both broad and specific terms the current state of knowledge in the general area of metabolic biochemistry, and to identify those areas that afford unusual opportunity or that are relatively underdeveloped in comparison with other areas of plant biology. Participants provided critiques of the state of knowledge of the major areas of metabolic biochemistry in relation to a series of questions that are presented herein.

  9. Biochemistry on the game board: improvements in the teaching-learning process in basic education

    OpenAIRE

    2015-01-01

    According to the Curriculum Guidelines of Biology of Basic Education "school should encourage the pedagogical practice based on different methodologies, valuing the teaching concepts, the learning (internalization) and the evaluation that allow teachers and students being aware of the need for emancipatory transformation”.  The teaching of biochemistry and its contents in basic education are not discussed as a structuring content within the Biology subject, but are included in related content...

  10. The genome sequence of Barbarea vulgaris facilitates the study of ecological biochemistry

    DEFF Research Database (Denmark)

    Byrne, Stephen L.; Erthmann, Pernille Østerbye; Agerbirk, Niels

    2017-01-01

    The genus Barbarea has emerged as a model for evolution and ecology of plant defense compounds, due to its unusual glucosinolate profile and production of saponins, unique to the Brassicaceae. One species, B. vulgaris, includes two ‘types’, G-type and P-type that differ in trichome density, and t...... deter larvae to the extent that they die. The B. vulgaris genome will promote the study of mechanisms in ecological biochemistry to benefit crop resistance breeding....

  11. BIOCHEMISTRY APPROACH IN BOOKS APPROVED BY THE NATIONAL BOOK TEACHING PROGRAM 2015

    Directory of Open Access Journals (Sweden)

    C. E.S. Rocha

    2015-08-01

    Full Text Available INTRODUCTION: Collections of textbooks are distributed every three years to basic education schools and represent a source of knowledge to students and a reference to the pedagogical work of teachers. Biochemistry is an area that studies the chemical processes occurring in living organisms, with their concepts present in both chemistry textbooks as in biology. It is necessary that the present approach in these books are interesting to students, to recognize the importance of understanding these processes. OBJECTIVES: To analyze as it has been crafted in biochemistry textbooks high school approved by PNLD 2015. MATERIALS AND METHODS: We investigated how the biochemical area-related content are described in the book "Bio - Volume 1", authored by Sonia Lopes and Sergio Rosso, and in the book "Chemistry - Volume 3" by Martha Reis with regard to clarity of concepts, context, illustrations and interdisciplinarity. RESULTS: Both books have chapters devoted especially to describe on this area, with plenty of catchy titles, but one in the first year and the other in the final year of high school. In biology textbook chapter called "The chemistry of life" brings in its approach clear and succinct concepts, contextualizes the issues on current information of figures and tables, features lots of illustrations that range from photographs to represent chemical structures and is mainly interdisciplinary in test questions. In chemistry textbook chapter "Introduction to Biochemistry" shows clear and detailed concepts, contextualizes with curiosities and everyday issues of students, has auxiliary illustrations in understanding and is interdisciplinary. CONCLUSIONS: We realize that there is a gap between the disciplines to work biochemistry during high school, but the form of presentation used for books is clear and present use of languages in the daily lives of students, making learning interesting and attractive.

  12. BIOCHEMISTRYGUIDE: A GUIDE TO STUDY BIOCHEMISTRY IN THE PALM OF YOUR HAND

    Directory of Open Access Journals (Sweden)

    M. L.C. Zago

    2015-08-01

    Full Text Available The new generation has grown driven by the digital technologies. Nowadays, the access to information has extended to the mobile technologies, like cell phones and tablets, increasing the technological resources for use in education. Educational institutions are opening more and more space to technologies, which have a new teaching, and learning methodology. The internet is an inexhaustible source of knowledge. With so much available material, the student finds difficulties in choosing the best source to study. Develop a cell phone app that works as a biochemistry study guide, providing materials from safe source and easy access. The app was developed at the website "Factory of apps", for free, and available for all the operational systems (Android, IOS, windows, enabling the download in any device. Were inserted in it materials like: summaries of the main subjects dealt in the discipline with clear and objective language, scientific articles to complementary reading, video-classes and exercises. Offering fast and practical access to the material, facilitating the process of studying and learning. The student does not have to let his mobile phone in preference of studying and does not find difficulties to filter available information in the internet. After the app's use, was applied a questionnaire to the participating students for this tool's assessment, such as layout, quality of the available material, access facilities. All the participants have approved the listed aspects and have said that the use of the tool can help at the biochemistry study, by the reliable material and by being available in a mobile device providing quick and immediate access, at any time in the palm of the hand. The use of this tool can be applied in the classroom to help the professor in getting materials and to help the students by its practicality.Acknowledgements: CnpQ, Unipampa. Keywords: App, BiochemistryGuide, Biochemistry

  13. Utilizing Unnatural Amino Acids to Illustrate Protein Structure-Function Relationships: An Experiment Designed for an Undergraduate Biochemistry Laboratory

    Science.gov (United States)

    Maza, Johnathan C.; Villa, Jordan K.; Landino, Lisa M.; Young, Douglas D

    2016-01-01

    The site-specific introduction of unnatural amino acids (UAAs) has been demonstrated to be a useful tool in protein engineering. Moreover, the incorporation of a UAA into a protein has become feasible with the increased commercial availability of UAAs and robust expression plasmids. In addition to the ease of incorporation, the concepts utilized…

  14. Designing Laboratory Exercises for the Undergraduate Molecular Biology/Biochemistry Student: Techniques and Ethical Implications Involved in Personalized Medicine

    Science.gov (United States)

    Weinlander, Kenneth M.; Hall, David J.

    2010-01-01

    Personalized medicine refers to medical care that involves genetically screening patients for their likelihood to develop various disorders. Commercial genome screening only involves identifying a consumer's genotype for a few single nucleotide polymorphisms. A phenotype (such as an illness) is greatly influenced by three factors: genes, gene…

  15. EVALUATION OF A SUMMER BIOCHEMISTRY COURSE SETTLED BY GRADUATE STUDENTS (PMBqBM/SBBq AIMING UNDERGRADUATE STUDENTS

    Directory of Open Access Journals (Sweden)

    D. G. Pompeu

    2015-08-01

    Full Text Available The good development of research in biochemistry is strongly linked to the knowledge and skill of the professional in using the techniques available to perform the research. To do this, the researcher must have adequate training and the period of initiation to science during graduation is of fundamental importance for the formation of this professional. Graduate students, in its turn, must seek to strengthen their knowledge and development of their teaching skills. In this scenario, the Multicenter Postgraduate Program in Biochemistry and Molecular Biology (PMBqBM - (Brazilian Society for Biochemistry and Molecular Biology - SBBq - and Federal University of São João del Rei, Campus Midwest - UFSJ/CCO joined the Postgraduate Program in Biotechnology (UFSJ/CCO for the creation of I Summer Course in Biochemistry, Molecular Biology and Biotechnology of UFSJ. The objective was to promote the dissemination of the research areas in biochemistry present in UFSJ as well as both Postgraduate programs. This course was organized and taught by students of the two Postgraduate programs and exclusively aimed to undergraduate students from other institutions. In it, undergraduate students could choose from 12 courses of different lines of research linked to the areas of Biochemistry, Molecular Biology and Biotechnology established at UFSJ. The course, lasting 40 hours, had 80 subscribers for a total of 61 vacancies. Registrants were from 8 different Brazilian states and 12 different graduate degree courses, especially Biology. The course had average rating of 9.4, and the practical activities reached the rate of 100% positive evaluations. Through the observation of results obtained, it is concluded that the course met its main objectives to strengthen ties between institutions where develop research in biochemistry, to promote teaching and training techniques, as well as disclose the Postgraduate courses on Biochemistry, especially PMBqBM.

  16. 21 CFR 225.158 - Laboratory assays.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false Laboratory assays. 225.158 Section 225.158 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS... Laboratory assays. Where the results of laboratory assays of drug components, including assays by State...

  17. Thalidomide Makes a Comeback: A Case Discussion Exercise That Integrates Biochemistry and Organic Chemistry

    Science.gov (United States)

    Bennett, Nicole; Cornely, Kathleen

    2001-06-01

    The case discussion method, which involves teaching scientific theory in a framework that students can relate to their own world, is an interdisciplinary pedagogical tool. Therefore, case study exercises can be used to integrate biochemistry with other advanced chemistry courses. The case presented here can be used at the end of a second-semester organic chemistry course or in an introductory biochemistry course. The case is a fact-based, fictional story in which an FDA official must decide whether to carry out the agency's threat to shut down several buyers clubs that import thalidomide from overseas and dispense it to their members for the treatment of AIDS. Students are required to read the body of the case, analyze data, and search for information using limited leads. Using well-considered arguments based on their research, they are asked to come to conclusions about how the element of risk involved in thalidomide distribution is assessed. They apply their knowledge of biochemistry to assess how thalidomide acts at the cellular level and they apply their knowledge of organic chemistry in writing mechanisms of thalidomide hydrolysis and in the design of thalidomide analogs. Students are assessed on their ability to work in groups, to critically analyze scientific data, and to develop public policies based on risk-benefit analysis.

  18. Biochemistry students' ideas about how an enzyme interacts with a substrate.

    Science.gov (United States)

    Linenberger, Kimberly J; Bretz, Stacey Lowery

    2015-01-01

    Enzyme-substrate interactions are a fundamental concept of biochemistry that is built upon throughout multiple biochemistry courses. Central to understanding enzyme-substrate interactions is specific knowledge of exactly how an enzyme and substrate interact. Within this narrower topic, students must understand the various binding sites on an enzyme and be able to reason from simplistic lock and key or induced fit models to the more complex energetics model of transition state theory. Learning to understand these many facets of enzyme-substrate interactions and reasoning from multiple models present challenges where students incorrectly make connections between concepts or make no connection at all. This study investigated biochemistry students' understanding of enzyme-substrate interactions through the use of clinical interviews and a national administration (N = 707) of the Enzyme-Substrate Interactions Concept Inventory. Findings include misconceptions regarding the nature of enzyme-substrate interactions, naïve ideas about the active site, a lack of energetically driven interactions, and an incomplete understanding of the specificity pocket.

  19. The Proteomics Stock Market Project. A Cross-Disciplinary Collaboration in Biochemistry and Business Education

    Science.gov (United States)

    Keller, Heath; Cox, James R.

    2004-04-01

    Students taking courses in different disciplines can work together to add unique elements to their educational experience. A model for this type of pedagogical approach has been established in the Proteomics Stock Market Project, a collaborative effort between instructors and students in the Department of Chemistry and Department of Management, Marketing, and Business Administration at Murray State University. Stage I involved biochemistry students investigating the topic of proteomics and choosing companies for potential investment based only on scientific investigation. Marketing and management students completed Stage II and provided an investment analysis on the companies selected in Stage I. In Stage III, the biochemistry students focused on a particular company and investigated a protein-based therapeutic product. Blackboard software was utilized in each stage of the project to facilitate the exchange of information and electronic documents. This project was designed to give biochemistry students an appreciation for the emerging field of proteomics and the marketing and management students a flavor for real-world applications of business principles. During the project, students were exposed to ideas and concepts not typically covered in their courses. With this involvement, the students had the opportunity to gain a broader perspective of course content compared to a more traditional curriculum.

  20. Genomics and Biochemistry of Saccharomyces cerevisiae Wine Yeast Strains.

    Science.gov (United States)

    Eldarov, M A; Kishkovskaia, S A; Tanaschuk, T N; Mardanov, A V

    2016-12-01

    Saccharomyces yeasts have been used for millennia for the production of beer, wine, bread, and other fermented products. Long-term "unconscious" selection and domestication led to the selection of hundreds of strains with desired production traits having significant phenotypic and genetic differences from their wild ancestors. This review summarizes the results of recent research in deciphering the genomes of wine Saccharomyces strains, the use of comparative genomics methods to study the mechanisms of yeast genome evolution under conditions of artificial selection, and the use of genomic and postgenomic approaches to identify the molecular nature of the important characteristics of commercial wine strains of Saccharomyces. Succinctly, data concerning metagenomics of microbial communities of grapes and wine and the dynamics of yeast and bacterial flora in the course of winemaking is provided. A separate section is devoted to an overview of the physiological, genetic, and biochemical features of sherry yeast strains used to produce biologically aged wines. The goal of the review is to convince the reader of the efficacy of new genomic and postgenomic technologies as tools for developing strategies for targeted selection and creation of new strains using "classical" and modern techniques for improving winemaking technology.

  1. 21 CFR 58.49 - Laboratory operation areas.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Laboratory operation areas. 58.49 Section 58.49... LABORATORY PRACTICE FOR NONCLINICAL LABORATORY STUDIES Facilities § 58.49 Laboratory operation areas. Separate laboratory space shall be provided, as needed, for the performance of the routine and...

  2. Virtual Laboratories

    Science.gov (United States)

    Hut, P.

    At the frontier of most areas in science, computer simulations playa central role. The traditional division of natural science into experimental and theoretical investigations is now completely outdated. Instead, theory, simulation, and experimentation form three equally essential aspects, each with its own unique flavor and challenges. Yet, education in computational science is still lagging far behind, and the number of text books in this area is minuscule compared to the many text books on theoretical and experimental science. As a result, many researchers still carry out simulations in a haphazard way, without properly setting up the computational equivalent of a well equipped laboratory. The art of creating such a virtual laboratory, while providing proper extensibility and documentation, is still in its infancy. A new approach is described here, Open Knowledge, as an extension of the notion of Open Source software. Besides open source code, manuals, and primers, an open knowledge project provides simulated dialogues between code developers, thus sharing not only the code, but also the motivations behind the code.

  3. AN ALTERNATIVE STRATEGY TO ANALYZE THE CONTENTS OF BIOCHEMISTRY INTRODUCTORY COURSES

    Directory of Open Access Journals (Sweden)

    A.K. Miskalo

    2008-05-01

    Full Text Available A common problem  educators  from  different areas face  is  to  fit the increasing  amount  of information  with the maintenance  and/or,  not seldom,  a decrease in the class load of their courses. This actual situation  necessarily forces the educator  to severely select the topics to be worked out. In the cur rent scenario of most teaching institutions, this decision  is  taken by the teacher.  In order to do this, a  list of the topics considered to be essential  for an appropriate biochemistry course is necessary. Taking for granted that questions from biochemistry courses tests reflect  the topics  considered most relevant by teachers,  questions from different courses offered by Biochemistry Department of USP were analyzed. The objective of this analysis  was  to  answer two main questions, namely (1  Which is the exte nt and  depth  of the common topics  in  biochemistry  introductory courses? and (2  Are there (and ,  if  there are,  which are they?  specific topics  for  different careers?  The  method we adopted was to verify  the demanded topics  in  written tests and  to  classify  their  cognitive level according to Bloom’s Taxonomy.  The most recurring topics found are Protein Structure and Metabolism Regulation. The results indicate a strong predominance of  low-level categories  (Knowledge e Comprehension,  with little  occurrence  of high-level categories (from Application on.  It is expected, from further development of this study,  to outline  the topics considered  relevant  to  set the basis for  the discussion  on the establishment of a minimum curriculum for biochemistry courses. Key words: Bloom’s Taxonomy, minimum curriculum, written tests analysis.

  4. Laboratory Activities

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Christopher F.; Serne, R. Jeffrey

    2008-01-17

    This chapter summarizes the laboratory activities performed by PNNL’s Vadose Zone Characterization Project in support of the Tank Farm Vadose Zone Program, led by CH2M HILL Hanford Group, Inc. The results of these studies are contained in numerous reports (Lindenmeier et al. 2002; Serne et al. 2002a, 2002b, 2002c, 2002d, 2002e; Lindenmeier et al. 2003; Serne et al. 2004a, 2004b; Brown et al. 2005, 2006a, 2007; Serne et al. 2007) and have generated much of the data reported in Chapter 22 (Geochemistry-Contaminant Movement), Appendix G (Geochemistry-Contaminant Movement), and Cantrell et al. (2007, SST WMA Geochemistry Data Package – in preparation). Sediment samples and characterization results from PNNL’s Vadose Zone Characterization Project are also shared with other science and technology (S&T) research projects, such as those summarized in Chapter 12 (Associated Science Activities).

  5. Ion Exchange and Thin Layer Chromatographic Separation and Identification of Amino Acids in a Mixture: An Experiment for General Chemistry and Biotechnology Laboratories

    Science.gov (United States)

    Brunauer, Linda S.; Caslavka, Katelyn E.; Van Groningen, Karinne

    2014-01-01

    A multiday laboratory exercise is described that is suitable for first-year undergraduate chemistry, biochemistry, or biotechnology students. Students gain experience in performing chromatographic separations of biomolecules, in both a column and thin layer chromatography (TLC) format. Students chromatographically separate amino acids (AA) in an…

  6. Actualization and perspectives of Bachelor degree in Biochemistry from Universidade Federal de Viçosa(UFV - (Symposium SBBq Brazil

    Directory of Open Access Journals (Sweden)

    J. L. R. Fietto

    2013-05-01

    Full Text Available The Bachelor Degree in Biochemistry of the UFV was  created in 2001 by the Biochemistry and Molecular Biology Department as a  groundbreaking initiative at Brazil. The program was an attempt to assume both academic and biotechnology sectors, preparing capable professionals for the development of new products and biochemical process. Our initial challenge came with the ingress of the first classes bringing doubts related to their formation and action profiles, sometimes confused with clinical analysts or pharmacists. Lecture series with professionals was used to demonstrate the present scenario with a growing market share, instigating discussions about the pedagogic project. The secondchallenge was the student’s pressure to recognition of under graduation programby the Brazilian Education Ministry (MEC and the biochemical professional recognition by one Regional Council. The bachelor degree was recognized by the MEC in 2004 and the Regional Council of Chemistry became our major partner. The  success of the initiative of the UFV has been giving good results such as the participation in the formation process of the Bachelor Degree in Biochemistry of the Federal University of São João Del Rei (UFSJ and stimulated the creation of another biochemistry course at State University of Maringá (UEM. The success of our students in Phd programs spread in Brazil evidences the quality of Biochemistry bachelors from UFV. Now the egresses have been recognized by biochemistry and biotechnology industry. However we still face problems with impaired recognition in many federal concourses that do not recognize the Biochemist as a chemistry and biotechnology professional. With high expectative, we wait that the biochemistry bachelordegree spreads widely in the country making possible the opening of new opportunities in all ways.

  7. Bio Engineering Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — Description/History: Chemistry and biology laboratoriesThe Bio Engineering Laboratory (BeL) is theonly full spectrum biotechnology capability within the Department...

  8. Bio Engineering Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — Description/History: Chemistry and biology laboratories The Bio Engineering Laboratory (BeL) is theonly full spectrum biotechnology capability within the Department...

  9. FOOTWEAR PERFORMANCE LABORATORY

    Data.gov (United States)

    Federal Laboratory Consortium — This laboratory provides biomechanical and physical analyses for both military and commercial footwear. The laboratory contains equipment that is integral to the us...

  10. Advanced Chemistry Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — Description/History: Chemistry laboratory The Advanced Chemistry Laboratory (ACL) is a unique facility designed for working with the most super toxic compounds known...

  11. Physical Sciences Laboratory (PSL)

    Data.gov (United States)

    Federal Laboratory Consortium — PNNL's Physical Sciences Laboratory (PSL) houses 22 research laboratories for conducting a wide-range of research including catalyst formulation, chemical analysis,...

  12. Distributed Energy Technology Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Distributed Energy Technologies Laboratory (DETL) is an extension of the power electronics testing capabilities of the Photovoltaic System Evaluation Laboratory...

  13. Pheochromocytoma after Cesarean Section

    Science.gov (United States)

    Naghshineh, Elham; Shahraki, Azar Danesh; Sheikhalian, Somaye; Hashemi, Leila

    2016-01-01

    Pheochromocytoma is a catecholamine-producing tumor. There are a very few reported cases of clinical pheochromocytoma. Here, we report a 27-year-old woman para 1 live 1 with chief complaint of headache, confusion, nausea, and vomiting 2 days after cesarean section. She was anxious and had palpitation. On physical examination, fever, tachycardia, tachypnea, high blood pressure, and right thyroid nodule were found. She was managed as pregnancy-induced hypertension at first. In laboratory data, epinephrine, norepinephrine, metanephrine, normetanephrine, and vanillylmandelic acid were increased in 24 h urine collection. An adrenal mass was detected in abdominal computed tomography. Regarding clinical and paraclinical findings, pheochromocytoma was diagnosed. The patient received medical treatment, but it was not effective; hence, she underwent adrenalectomy. PMID:27076898

  14. Pheochromocytoma after cesarean section

    Directory of Open Access Journals (Sweden)

    Elham Naghshineh

    2016-01-01

    Full Text Available Pheochromocytoma is a catecholamine-producing tumor. There are a very few reported cases of clinical pheochromocytoma. Here, we report a 27-year-old woman para 1 live 1 with chief complaint of headache, confusion, nausea, and vomiting 2 days after cesarean section. She was anxious and had palpitation. On physical examination, fever, tachycardia, tachypnea, high blood pressure, and right thyroid nodule were found. She was managed as pregnancy-induced hypertension at first. In laboratory data, epinephrine, norepinephrine, metanephrine, normetanephrine, and vanillylmandelic acid were increased in 24 h urine collection. An adrenal mass was detected in abdominal computed tomography. Regarding clinical and paraclinical findings, pheochromocytoma was diagnosed. The patient received medical treatment, but it was not effective; hence, she underwent adrenalectomy.

  15. Abstracts of the 26. Annual meeting of the Brazilian Society on Biochemistry and Molecular Biology; Resumos da 26. reuniao anual da Sociedade Brasileira de Bioquimica e Biologia Molecular

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-07-01

    This meeting was about biochemistry and molecular biology. It was discussed topics related to bio energetic, channels, transports, biotechnology, metabolism, cellular biology, immunology, toxicology, photobiology and pharmacology.

  16. Abstracts of the 27. Annual meeting of the Brazilian Society on Biochemistry and Molecular Biology; Resumos da 27. reuniao anual da Sociedade Brasileira de Bioquimica e Biologia Molecular

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-07-01

    This meeting was about biochemistry and molecular biology. It was discussed topics related to bio energetic, channels, transports, biotechnology, metabolism, cellular biology, immunology, toxicology, photobiology and pharmacology.

  17. Research programs at the Department of Energy National Laboratories. Volume 2: Laboratory matrix

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-12-01

    For nearly fifty years, the US national laboratories, under the direction of the Department of Energy, have maintained a tradition of outstanding scientific research and innovative technological development. With the end of the Cold War, their roles have undergone profound changes. Although many of their original priorities remain--stewardship of the nation`s nuclear stockpile, for example--pressing budget constraints and new federal mandates have altered their focus. Promotion of energy efficiency, environmental restoration, human health, and technology partnerships with the goal of enhancing US economic and technological competitiveness are key new priorities. The multiprogram national laboratories offer unparalleled expertise in meeting the challenge of changing priorities. This volume aims to demonstrate each laboratory`s uniqueness in applying this expertise. It describes the laboratories` activities in eleven broad areas of research that most or all share in common. Each section of this volume is devoted to a single laboratory. Those included are: Argonne National Laboratory; Brookhaven National Laboratory; Idaho National Engineering Laboratory; Lawrence Berkeley Laboratory; Lawrence Livermore National Laboratory; Los Alamos National Laboratory; National Renewable Energy Laboratory; Oak Ridge National Laboratory; Pacific Northwest Laboratory; and Sandia National Laboratories. The information in this volume was provided by the multiprogram national laboratories and compiled at Lawrence Berkeley Laboratory.

  18. A proposal of collaborative education for biochemistry and cell biology teaching

    Directory of Open Access Journals (Sweden)

    A. A. Souza-Júnior

    2015-08-01

    Full Text Available INTRODUCTION: Currently students grow up in a world of digital tools that allow you to connect instantly with the world. At the same time, teachers face several challenges to increase student interest and learning efficiency. One such challenge is the pedagogical commitment of the density of biochemistry and cell biology contents, producing a conflict scenario, between meeting content and maintain the class quality. OBJECTIVES: From this perspective, this study aimed to evaluate the learning biochemistry and cell biology contents in high school classes of IFRN, using collaborative and digital tools in the Moodle. MATERIAL AND METHODS: The contents were offered using various tools such as video lectures, forums, questionnaires, portfolios, glossaries and electronic books. Then these tools were evaluated using an electronic form.  In addition to the tools, we evaluated the platform interaction, the performance of activities and the content gamification. RESULTS: The quantitative results revealed directly proportional relationship of the interaction of Moodle with the performance of activities. The content gamification was also assessed positively, with 61% of students considered good, very good or excellent. The best evaluated tools were video lectures, with 31% preference, and questionnaires, with 24%; followed by electronic book, with 10%, and portfolio, with 5.5%. The other tools totaled 30% of the preference. Qualitative results revealed an educational gain of content, because the student lived the experience of teaching and learning collaboratively. In addition, these tools decreased conflicts between content and schedule. CONCLUSION: Thus, the use of information and communication technology (ICT in a collaborative learning provides relevant results, bringing the reality of the world connected to the classroom. In addition, it assists in defining the content and creative development of a strategy for the construction of the concepts applied

  19. Hematology and serum biochemistry of Indian spectacled cobra (Naja naja) and Indian rat snake (Ptyas mucosa)

    Science.gov (United States)

    Muliya, Sanath Krishna; Bhat, Mudraje Narayana

    2016-01-01

    Aim: To study the hematology and serum biochemistry parameters of Indian spectacled cobra (Naja naja) and Indian rat snake (Ptyas mucosa) and to evaluate the differences in the same between captive and wild populations. Materials and Methods: Animals were categorized into four groups, viz., wild Indian spectacled cobra (n=10), wild Indian rat snakes (n=10), captive Indian spectacled cobra (n=10), and captive Indian rat snake (n=10). The snakes were restrained with restraint tubes, and 2 ml of blood was collected from either heart or ventral coccygeal vein. Hematological examinations were performed manually and serum biochemistry assays were performed on semi-automated clinical chemistry analyzer. Results: The values of total erythrocyte count, packed cell volume, and hemoglobin were slightly low in captive spectacled cobras and captive rat snakes compared to wild ones, whereas total leukocyte count was found to be slightly high in wild spectacled cobras compared to captive ones. All the recorded values of biochemical and electrolyte analytes were found to be well within expected range for snakes except for total protein and chloride levels in both the species which was slightly above the expected range. Conclusion: The hematology and serum biochemistry intervals of the two most common Indian snakes are presented here. The data will be useful in routine health evaluations and aiding in better medical management of the species studied. Since this study is the first to report complete hematologic and blood biochemical ranges for the study species, observations made here can also be used as referral intervals for future use. PMID:27651683

  20. The Use of Information and CommunicationTechnologies in Biochemistry Teaching

    Directory of Open Access Journals (Sweden)

    C. Munford

    2011-04-01

    Full Text Available The gap between traditional tools for teaching and the modern multimedia languages leads us to search for new methodologies of teaching. Using the Moodlesoftware as a Virtual Learning Environment (VLE, we give the students the opportunity to exchange experiences and research results, participate on debates and see animations. This work  was meant to createa VLE and investigate its impact  on the Biochemistry teaching-learning process. Besides observing students’ perception and attitude towards the VLE with questionnaires and Moodle’s statistical data, quantitative and qualitative studies were developed to understand the contribution of animations available in the VLE for the understanding of Biochemistry topics. The results showed that undergraduate students improved their academic results. Students mentioned that the biggest contribution of the VLE and animations was to make the study content more interesting and dynamic, motivating them tostudy harder. Moreover, students using only animations to study can achieve levels of knowledge similar to the ones obtained in traditional classes. Interestingly, our analysis also showed that watching animations and then attending to a traditional class makes students reach a level of knowledge that is impossible to reach only in classroom. This work demonstrates that the use of Information andCommunication Technologies (ICTs can be positive in the pedagogical processesinvolved in Biochemistry teaching, and they should be better spread amongst teaching institutions.

  1. Enigma: A Board Game As A Potential Tool For Biochemistry And Microbiology Learning And Integration

    Directory of Open Access Journals (Sweden)

    V. S. S. Alpim

    2009-05-01

    Full Text Available Biochemistry and microbiology is a basic discipline for many gradua tion courses of the biomedical a rea, such as Medicine, Odontology and Biomedicine.  The abstract concepts of these sciences turn out to be a major difficulty for the learning/teaching relation in the classes.  Nevertheless, alternative strategies used inside or outside the classrooms could also make learning results better. In 2008, Odontology students from FTC prepared games based on biochemistry and microbiology and presented them in groups.   Our group developed a game based on a classic board game known in Brazil as Perfil®, whic h was called Enigma. The objective of the game is to reach the end of the board by moving a piece through it. To move your piece  on the board  you have to discoverenigmas written on card. You can ask for up to 20 clues  to  your opponent to discover the enigma. For each clue given you lose a point and your opponent earns one, so that after you  guess correctly, you get to move your piece on the board a number equal to 20 minus the clues you were given .  The preparation and presentation of this and other games w as used as means of evaluating students development in microbiology and biochemistry, but also improved understanding and integration of such areas. Teachers and studentsacknowledged Enigma as a good game for increasing their experience in the basic areas.

  2. Reference values of hematology, biochemistry, and blood type in cynomolgus monkeys from cambodia origin.

    Science.gov (United States)

    Choi, Kangmoo; Chang, Jaejin; Lee, Min-Jae; Wang, Seungsu; In, Kimhong; Galano-Tan, Wilhelm C; Jun, Sanghun; Cho, Kahee; Hwang, Yong-Hwa; Kim, Sung-Ju; Park, Wanje

    2016-03-01

    Cynomolgus monkeys as nonhuman primates are valuable animal models because they have a high level of human gene homology. There are many reference values for hematology and biochemistry of Cynomolgus monkeys that are needed for proper clinical diagnosis and biomedical research conduct. The body weight information and blood type are also key success factors in allogeneic or xenogeneic models. Moreover, the biological parameters could be different according to the origin of the Cynomolgus monkey. However, there are limited references provided, especially of Cambodia origin. In this study, we measured average body weight of 2,518 Cynomolgus monkeys and analyzed hematology and serum biochemistry using 119 males, and determined blood types in 642 monkeys with Cambodia origin. The average body weight of male Cynomolgus monkeys were 2.56±0.345 kg and female group was 2.43±0.330 kg at the age from 2 to 3 years. The male group showed relatively sharp increased average body weight from the 3 to 4 age period compared to the female group. In hematology and biochemistry, it was found that most of the data was similar when compared to other references even though some results showed differences. The ABO blood type result showed that type A, B, AB, and O was approximately 15.6, 33.3, 44.2, and 6.9%, respectively. The main blood type in this facility was B and AB. These biological background references of Cambodia origin could be used to provide important information to researchers who are using them in their biomedical research.

  3. EDUCACIONAL GAME OF LEARNING AS DIDATIC SUPPORT IN INTRODUCTION TO BIOCHEMISTRY DISCIPLINE

    Directory of Open Access Journals (Sweden)

    M. S. Vasconcelos

    2012-05-01

    Full Text Available The cellular respiration process is a crucial subject in biochemistry learning. The use of ludic tools appears to be a good strategy in education intensifying the teach and learning process. The aim of this work was to create and introduce a game as educational tool helping the students to construct the knowledge.  This work was accomplished in UFC involving three groups of 5 students of Introduction to Biochemistry discipline from Food Engineering course.  It was developed a board game  entitled:  The dynamics OXI-REDOXI in Respiratory Chain. The game consisted in a board with the scheme of the respiratory chain, chips to complete the gaps and 12 question cards. The students had previous classes about the subject and a survey right before  playing the game. The monitor explained the rules and the wrong answers of the students to question cards. During the game it was observed mistakes in the answers of the students to  question cards  and either  regarding placing the chips representing the reactions of the respiratory chain. The monitor stimulated the discussion among them and elucidated the doubts. The students demonstrated motivation and interest during the game.  The majority of the students found it easy to play and confirmed that it facilitate the learning process.  The application of the same survey after the game showed that they understood the  respiratory chain and the grades were at least 50% higher after the game.  This game reveals itself a dynamic and constructive tool for the learning  process in biochemistry.

  4. Hematology and serum biochemistry of Indian spectacled cobra (Naja naja and Indian rat snake (Ptyas mucosa

    Directory of Open Access Journals (Sweden)

    Sanath Krishna Muliya

    2016-08-01

    Full Text Available Aim: To study the hematology and serum biochemistry parameters of Indian spectacled cobra (Naja naja and Indian rat snake (Ptyas mucosa and to evaluate the differences in the same between captive and wild populations. Materials and Methods: Animals were categorized into four groups, viz., wild Indian spectacled cobra (n=10, wild Indian rat snakes (n=10, captive Indian spectacled cobra (n=10, and captive Indian rat snake (n=10. The snakes were restrained with restraint tubes, and 2 ml of blood was collected from either heart or ventral coccygeal vein. Hematological examinations were performed manually and serum biochemistry assays were performed on semi-automated clinical chemistry analyzer. Results: The values of total erythrocyte count, packed cell volume, and hemoglobin were slightly low in captive spectacled cobras and captive rat snakes compared to wild ones, whereas total leukocyte count was found to be slightly high in wild spectacled cobras compared to captive ones. All the recorded values of biochemical and electrolyte analytes were found to be well within expected range for snakes except for total protein and chloride levels in both the species which was slightly above the expected range. Conclusion: The hematology and serum biochemistry intervals of the two most common Indian snakes are presented here. The data will be useful in routine health evaluations and aiding in better medical management of the species studied. Since this study is the first to report complete hematologic and blood biochemical ranges for the study species, observations made here can also be used as referral intervals for future use.

  5. The coevolutionary roots of biochemistry and cellular organization challenge the RNA world paradigm.

    Science.gov (United States)

    Caetano-Anollés, Gustavo; Seufferheld, Manfredo J

    2013-01-01

    The origin and evolution of modern biochemistry and cellular structure is a complex problem that has puzzled scientists for almost a century. While comparative, functional and structural genomics has unraveled considerable complexity at the molecular level, there is very little understanding of the origin, evolution and structure of the molecules responsible for cellular or viral features in life. Recent efforts, however, have dissected the emergence of the very early molecules that populated primordial cells. Deep historical signal was retrieved from a census of molecular structures and functions in thousands of nucleic acid and protein structures and hundreds of genomes using powerful phylogenomic methods. Together with structural, chemical and cell biology considerations, this information reveals that modern biochemistry is the result of the gradual evolutionary appearance and accretion of molecular parts and molecules. These patterns comply with the principle of continuity and lead to molecular and cellular complexity. Here, we review findings and report possible origins of molecular and cellular structure, the early rise of lipid biosynthetic pathways and components of cytoskeletal microstructures, the piecemeal accumulation of domains in ATP synthase complexes and the origin and evolution of the ribosome. Phylogenomic studies suggest the last universal common ancestor of life, the 'urancestor', had already developed complex cellular structure and bioenergetics. Remarkably, our findings falsify the existence of an ancient RNA world. Instead they are compatible with gradually coevolving nucleic acids and proteins in interaction with increasingly complex cofactors, lipid membrane structures and other cellular components. This changes the perception we have of the rise of modern biochemistry and prompts further analysis of the emergence of biological complexity in an ever-expanding coevolving world of macromolecules.

  6. Correlation of Visceral Fat Area with Metabolic Risk Factors in Romanian Patients: A Cross-Sectional Study

    Directory of Open Access Journals (Sweden)

    Hâncu Anca

    2015-12-01

    Full Text Available Background and Aims: The aim of the present study was to investigate the relationship between visceral fat area (VFA, estimated by bioimpedance, and cardiovascular risk factors independent of BMI and waist circumference in a cohort of Romanian patients. Material and Methods: This was a cross-sectional study, in which were collected data from 751 patients ≥18 years of age from Cluj-Napoca. Anthropometric, biochemistry, body composition and medical history parameters were recorded from patients’ files. Results: Compared with the participants with VFA <100 cm2, those with VFA ≥100 cm2 had significantly worse levels of the laboratory parameters describing the glycemic metabolism, lipid metabolism and liver functions (p <0.05 for all. A higher percentage of participants with VFA ≥100 cm2 had diabetes, obesity, hypertension, hypertriglyceridemia and hypo-HDL cholesterolemia (p <0.05 for all. VFA was correlated with systolic and diastolic blood pressure, total and LDL-cholesterol levels, triglycerides, ALT, previous diagnosis of diabetes, hypertriglyceridemia and hypo-HDL cholesterolemia independent of BMI and waist circumference. Conclusions: Among this cohort of Romanian adults, an increasing level of visceral adiposity was correlated with worse lipid and glucose metabolism parameters as well as with increased levels of ALT, which probably reflects liver fat deposition.

  7. The Application of Surveying Biochemistry Light Instrument in the Reaction System of Starch with Mn3+

    Institute of Scientific and Technical Information of China (English)

    Gu Zhengbiao; Wu Jiagen

    2000-01-01

    The possibility of the application of Surveying Biochemistry Light Instrument (SBLI) in the reaction system of starch with Mn3+was studied. The results showed: neither starch nor initiator(Mn3+) could produce light because no factor that could produce light existed in them. But starch could produce light when it was acted by Mn3 + and the intensity of light depended on the kind and concentration of starch, the amount of Mn3 +, the temperature and pH of the reaction system. In addition, the intensity of light decreased with the reaction time because of the gradual disappearance of starch free radicals.

  8. Secrets from the microbiome: molecular biology meets microbiology meets histopathology...meets clinical biochemistry.

    Science.gov (United States)

    Young, Caroline; Quirke, Philip

    2015-11-01

    The microbiome is the collective term used to describe the bacteria, viruses, fungi and archaea that reside on and in the human body. The majority of these organisms are found within the large bowel. Mounting evidence suggests that changes in the microbiome may be associated with the development of colorectal cancer, a disease which affects 1.3 million people a year worldwide. Using colorectal cancer as an example, this article presents the inter-specialty collaborative approach to microbiome research and discusses the key role that clinical biochemistry is likely to play.

  9. Theme: Laboratory Facilities Improvement.

    Science.gov (United States)

    Miller, Glen M.; And Others

    1993-01-01

    Includes "Laboratory Facilities Improvement" (Miller); "Remodeling Laboratories for Agriscience Instruction" (Newman, Johnson); "Planning for Change" (Mulcahy); "Laboratory Facilities Improvement for Technology Transfer" (Harper); "Facilities for Agriscience Instruction" (Agnew et al.); "Laboratory Facility Improvement" (Boren, Dwyer); and…

  10. 42 CFR 493.1355 - Condition: Laboratories performing PPM procedures; laboratory director.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Condition: Laboratories performing PPM procedures; laboratory director. 493.1355 Section 493.1355 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) STANDARDS AND CERTIFICATION LABORATORY...

  11. The Postwar Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Meade, Roger Allen [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-11-17

    Recent discussion of project policy has met with a widespread feeling that important alternatives were not being properly considered. These alternatives will be discussed here from the point of view of research personnel concerned with formulation a laboratory policy based on the wartime experience of Los Alamos. This policy is discussed on the primary assumption that the national investment here in facilities, in tradition, and in the existence of an going research and development laboratory organization ought not to be lightly discarded, but also ought not to be wholly continued without reexamination under the new conditions of peace. Others will discuss this policy more broadly, and others will make the decision of continuation; but the purpose of the present document is to suggest a policy which might help answer the question of what to do with Los Alamos.It is the thesis of this document that fundamental research in fields underlying the military utilization of atomic energy ought to be separated from all development testing and production. It still remains to argue which of these separate functions this mesa should carry out. In the next sections it is proposed to describe what this laboratory can do and what it should stop trying to do, and on this detailed basis a general program is proposed.

  12. Biochemical software: Carbohydrates on Laboratory

    Directory of Open Access Journals (Sweden)

    D.N. Heidrich

    2005-07-01

    Full Text Available Educators around  the  world  are  being  challenged  to  develop  and  design  better and  more  effective strategies for student learning  using a variety  of modern  resources.  In this  present  work, an educa- tional  hypermedia  software  was constructed as a support tool to biochemistry teaching.  Occurrence, structure, main  characteristics and  biological  function  of the  biomolecule  Carbohydrates were pre- sented  through  modules.  The  software was developed  using concept  maps,  ISIS-Draw,  and  FLASH- MX animation program.  The chapter  Carbohydrates on Laboratory illustrates experimental methods of carbohydrates characterization, through  animation of a laboratory scenery.   The  subject was de- veloped showing reactions  as Bial, Benedict, Selliwanoff, Barfoed, Phenol  Sulphuric,  and Iodines, and also enzymatic  reactions  as glucose oxidase and amylase.  There are also links with short texts  in order to help the understanding of the contents  and principles of laboratory practice  as well as background reactions. Application of the software to undergraduate students and high school teachers  showed an excellent  acceptance.   All of them  considered  the  software  a very good learning  tool.  Both  teachers and students welcomed this program  as it is more flexible, and allows the learning in a more individual rhythm. In addition, application of the software would be suitable  to a more effective learning  and it is less expensive than conventional experimental teaching.

  13. Integrating Free Computer Software in Chemistry and Biochemistry Instruction: An International Collaboration

    Science.gov (United States)

    Cedeño, David L.; Jones, Marjorie A.; Friesen, Jon A.; Wirtz, Mark W.; Ríos, Luz Amalia; Ocampo, Gonzalo Taborda

    2010-10-01

    At the Universidad de Caldas, Manizales, Colombia, we used their new computer facilities to introduce chemistry graduate students to biochemical database mining and quantum chemistry calculations using freeware. These hands-on workshops allowed the students a strong introduction to easily accessible software and how to use this software to begin to explore computer modeling. Each workshop was scheduled for 2 h and each included a tutorial exercise to familiarize the students with the main menus and features of the software. In addition, accompanying lectures and practical laboratory sections were provided. Both courses were taught in Spanish although the written instructions were in English. This was not a problem since these students have a comfort level with reading English. Student feedback following these workshops was highly enthusiastic and positive. This international collaborative will impact both the teaching and research goals for this cohort of graduate students.

  14. Making Laboratories Count -- Better Integration of Laboratories in Physics Courses

    Science.gov (United States)

    Sizemore, Jim

    2011-10-01

    The quality of K-12 education leaves something to be desired and presents higher education faculty with the challenge of instructing under-prepared students. However, by their own admission, students from many institutions inform us that laboratory sections in science classes, including physics, consist mostly of showing up, going through the motions, and getting grades that boost their overall grade. This work presents laboratories that challenge students to take their laboratory work more seriously including specific rubrics enforcing SOLVE and Bloom's Taxonomy, pre-lab preparation work, and quizzes on pre-lab preparation. Early results are encouraging revealing greater student progress with better integration of laboratory with the rest of a complete physics course.

  15. Kinetic Biochemistry

    Directory of Open Access Journals (Sweden)

    Lorentz JÄNTSCHI

    2003-03-01

    Full Text Available Mathematics and computer programming have a major contribution to chemistry. Two directions can be identified: one that searches and tries (rich to explain the structural binding and shape of the chemical compounds [1] with major applications in QSPR/QSAR studies [2], and applied sciences such as engineering of materials or agriculture [3]; the second direction is to models the kinetic processes that are involved in chemical reactions [4]. Many such models are available here. The present paper describes three variants of well the known kinetic models and presents the mathematical equations associated with them. The differential equations are numerically solved and fitted with MathCad program. [1] Diudea M., Gutman I., Jäntschi L., Molecular Topology, Nova Science, Huntington, New York, 332 p., 2001, 2002. [2] Diudea M. V., Ed., QSPR / QSAR Studies by Molecular Descriptors, Nova Science, Huntington, New York, 438 p., 2001. [3] Jäntschi L., Microbiology and Toxicology. Phytochemistry Studies (in Romanian, Amici, Cluj-Napoca, 184 p., 2003. [4] Jäntschi L., Unguresan M., Physical Chemistry. Molecular Kinetic and Dynamic (in Romanian, Mediamira, Cluj-Napoca, 159 p., 2001.

  16. Using Adobe Flash animations of electron transport chain to teach and learn biochemistry.

    Science.gov (United States)

    Teplá, Milada; Klímová, Helena

    2015-01-01

    Teaching the subject of the electron transport chain is one of the most challenging aspects of the chemistry curriculum at the high school level. This article presents an educational program called "Electron Transport Chain" which consists of 14 visual animations including a biochemistry quiz. The program was created in the Adobe Flash CS3 Professional animation program and is designed for high school chemistry students. Our goal is to develop educational materials that facilitate the comprehension of this complex subject through dynamic animations which show the course of the electron transport chain and simultaneously explain its nature. We record the process of the electron transport chain, including connections with oxidative phosphorylation, in such a way as to minimize the occurrence of discrepancies in interpretation. The educational program was evaluated in high schools through the administration of a questionnaire, which contained 12 opened-ended items and which required participants to evaluate the graphics of the animations, chemical content, student preferences, and its suitability for high school biochemistry teaching.

  17. Krebs Roulette: Educative Game as Strategy of learning-teaching process in Biochemistry

    Directory of Open Access Journals (Sweden)

    C. N. Almada

    2010-05-01

    Full Text Available The Biochemistry study is complex and many times is restricted to memorization of theorical contents. The educational game could be an catalytic element, capable to contribute in the process of regaining the student's interest. The aim of this work was to create, apply and evaluate an educative game called “Krebs Roulette”. The game contents were 1 roulette, 3 board cards, 48 question cards and complementary game chips. In an experimental tryout, five students joined the test-play in the presence of a mediator. The game duration was one hour. The students were submitted to an evaluation under Krebs Cycle before and after the game. About the game strategy, the students noticed as positive points: easy to play, didactic, making the learn easier. The evaluation of the quizzes, even with a so small experimental group, indicated a significant increase in the teach-learning process. The achieved results suggest that the game could be used as complementary tool for comprehension of the Krebs Cycle as strategy of learning-teaching in Biochemistry.

  18. [Biochemistry for the benefit of humanity (practical achievements of my scientific work)].

    Science.gov (United States)

    Huliĭ, M F

    2005-01-01

    Science unites theory and practice, but theory is always in advance. Even our works (mentioned above) which are also important for practice and were awarded the State prizes could not be made without preliminary theoretical investigations. It should be said that our works with elaborated methods of therapy and drugs to treat chronic alcoholism, drug addiction, leucosis are rather of theoretical than of practical importance. Some our works which proved that carbon dioxide is the basis of life are also of especially great theoretical value. The paper deals with the investigations devoted to the problems of biochemistry in cattle breeding (the raising of fat content in milk; elaboration of the efficient method of fodder ensilage; raising of milk yield using the drug "Karboxilin"; development of the methods of isolation of crystalline glucose-oxidase and catalase used for clarifying blood) as well as to the problems of biochemistry in medicine (creation of the drug "Microcid", antileucosis drug "Corectin", drugs "Medichronal" and "Medicit" for treating alcoholism and drug addiction, drug "Namacit" for hindering the organism aging). Great attention is given to the problem of relations between the theoretical conception concerning the importance of CO2 in vital activity of human and animal organism and production of new drugs.

  19. Blood Biochemistry and Plasma Corticosterone Concentration in Broiler Chickens Under Heat Stress

    Directory of Open Access Journals (Sweden)

    Elvis Alexander Díaz López

    2014-07-01

    Full Text Available High ambient temperatures cause susceptibility to heat stress in broiler chickens, generating metabolic changes. This paper seeks to determine the changes in blood biochemistry and plasma corticosterone concentration, as well as in glucose, total protein, albumin, globulin, sodium, chlorine, potassium, magnesium, phosphorus, and calcium in broiler chickens under chronic heat stress and at ambient temperature conditions at the Colombian Amazonian piedmont. 21-days-old male chickens of two lines were studied, distributed in an unrestricted random design, in a two-factor scheme, with four treatments. Five repetitions per treatment were performed, and 25 animals per experimental unit examined. Broilers were fed a basic diet of corn and soybean meal with 3,100 kcal ME and 19.5% protein until they reached 42 days of age. The line factor had no effect on the evaluated variables (p ≥ 0.05. However, there was statistically significant difference (p ≤ 0.05 in all variables when concentrations of metabolites in broilers under chronic heat stress were compared to those of chickens exposed to ambient temperatures at the Colombian Amazon piedmont. In conclusion, blood biochemistry suffered significant changes under both experimental temperatures, with more physiological detriment in broilers under chronic heat stress. Concentration of corticosterone became the most sensitive and consistent indicator of the physiological condition of chronic heat stress.

  20. Anatomy and history of an external quality assessment program for interpretative comments in clinical biochemistry.

    Science.gov (United States)

    Vasikaran, Samuel D

    2015-05-01

    The provision of clinical interpretation of results, either verbally or in the printed report, may be considered an integral part of clinical biochemistry diagnostic service. Proficiency testing or external quality assessment (EQA) of such activity may be useful in education, training, continuing professional development and ensuring the quality of such service. Details of the Patient Report Comments Program (RPCProgram) developed by the Royal College of Pathologists of Australasia (RCPA) Chemical Pathology Quality Assurance Programs Pty Ltd (QAP) is described in this review. The program is aimed at pathologists, clinical scientists and trainees. Registered participants are provided a report with case details and a set of clinical biochemistry results at monthly intervals and submit an interpretative comment for the report. Comments received are broken up into components that are translated into common key phrases. An expert panel evaluates the key phrases, classifies them according to appropriateness and drafts a suggested comment, a case summary and a rationale, which are included in a summary report returned to participants. There is considerable diversity in the quality of interpretative comments received from participants of the PRCProgram. The primary purpose of EQA of interpretative commenting is educational self-assessment, and they are recognized as a continuing professional development activity. Whilst there is some evidence for the utility of interpretative comments in improving patient outcomes, evidence for the utility of EQA in improving quality of comments is awaited.

  1. Introduction to the Minireview Series on Modern Technologies for In-cell Biochemistry.

    Science.gov (United States)

    Lutsenko, Svetlana

    2016-02-19

    The last decade has seen enormous progress in the exploration and understanding of the behavior of molecules in their natural cellular environments at increasingly high spatial and temporal resolution. Advances in microscopy and the development of new fluorescent reagents as well as genetic editing techniques have enabled quantitative analysis of protein interactions, intracellular trafficking, metabolic changes, and signaling. Modern biochemistry now faces new and exciting challenges. Can traditionally "in vitro" experiments, e.g. analysis of protein folding and conformational transitions, be done in cells? Can the structure and behavior of endogenous and/or non-tagged recombinant proteins be analyzed and altered within the cell or in cellular compartments? How can molecules and their actions be studied mechanistically in tissues and organs? Is personalized cellular biochemistry a reality? This thematic series summarizes recent studies that illustrate some first steps toward successfully answering these modern biochemical questions. The first minireview focuses on utilization of three-dimensional primary enteroids and organoids for mechanistic studies of intestinal biology with molecular resolution. The second minireview describes application of single chain antibodies (nanobodies) for monitoring and regulating protein dynamics in vitro and in cells. The third minireview highlights advances in using NMR spectroscopy for analysis of protein folding and assembly in cells.

  2. An integrated biochemistry and genetics outreach program designed for elementary school students.

    Science.gov (United States)

    Ross, Eric D; Lee, Sarah K; Radebaugh, Catherine A; Stargell, Laurie A

    2012-02-01

    Exposure to genetic and biochemical experiments typically occurs late in one's academic career. By the time students have the opportunity to select specialized courses in these areas, many have already developed negative attitudes toward the sciences. Given little or no direct experience with the fields of genetics and biochemistry, it is likely that many young people rule these out as potential areas of study or career path. To address this problem, we developed a 7-week (~1 hr/week) hands-on course to introduce fifth grade students to basic concepts in genetics and biochemistry. These young students performed a series of investigations (ranging from examining phenotypic variation, in vitro enzymatic assays, and yeast genetic experiments) to explore scientific reasoning through direct experimentation. Despite the challenging material, the vast majority of students successfully completed each experiment, and most students reported that the experience increased their interest in science. Additionally, the experiments within the 7-week program are easily performed by instructors with basic skills in biological sciences. As such, this program can be implemented by others motivated to achieve a broader impact by increasing the accessibility of their university and communicating to a young audience a positive impression of the sciences and the potential for science as a career.

  3. Establishment of baseline haematology and biochemistry parameters in wild adult African penguins (Spheniscus demersus

    Directory of Open Access Journals (Sweden)

    Nola J. Parsons

    2015-03-01

    Full Text Available There are few publications on the clinical haematology and biochemistry of African penguins (Spheniscus demersus and these are based on captive populations. Baseline haematology and serum biochemistry parameters were analysed from 108 blood samples from wild, adult African penguins. Samples were collected from the breeding range of the African penguin in South Africa and the results were compared between breeding region and sex. The haematological parameters that were measured were: haematocrit, haemoglobin, red cell count and white cell count. The biochemical parameters that were measured were: sodium, potassium, chloride, calcium, inorganic phosphate, creatinine, cholesterol, serum glucose, uric acid, bile acid, total serum protein, albumin, aspartate transaminase and creatine kinase. All samples were serologically negative for selected avian diseases and no blood parasites were detected. No haemolysis was present in any of the analysed samples. Male African penguins were larger and heavier than females, with higher haematocrit, haemoglobin and red cell count values, but lower calcium and phosphate values. African penguins in the Eastern Cape were heavier than those in the Western Cape, with lower white cell count and globulin values and a higher albumin/globulin ratio, possibly indicating that birds are in a poorer condition in the Western Cape. Results were also compared between multiple penguin species and with African penguins in captivity. These values for healthy, wild, adult penguins can be used for future health and disease assessments.

  4. Establishment of baseline haematology and biochemistry parameters in wild adult African penguins (Spheniscus demersus).

    Science.gov (United States)

    Parsons, Nola J; Schaefer, Adam M; van der Spuy, Stephen D; Gous, Tertius A

    2015-03-25

    There are few publications on the clinical haematology and biochemistry of African penguins (Spheniscus demersus) and these are based on captive populations. Baseline haematology and serum biochemistry parameters were analysed from 108 blood samples from wild, adult African penguins. Samples were collected from the breeding range of the African penguin in South Africa and the results were compared between breeding region and sex. The haematological parameters that were measured were: haematocrit, haemoglobin, red cell count and white cell count. The biochemical parameters that were measured were: sodium, potassium, chloride, calcium, inorganic phosphate, creatinine, cholesterol, serum glucose, uric acid, bile acid, total serum protein, albumin, aspartate transaminase and creatine kinase. All samples were serologically negative for selected avian diseases and no blood parasites were detected. No haemolysis was present in any of the analysed samples. Male African penguins were larger and heavier than females, with higher haematocrit, haemoglobin and red cell count values, but lower calcium and phosphate values. African penguins in the Eastern Cape were heavier than those in the Western Cape, with lower white cell count and globulin values and a higher albumin/globulin ratio, possibly indicating that birds are in a poorer condition in the Western Cape. Results were also compared between multiple penguin species and with African penguins in captivity. These values for healthy, wild, adult penguins can be used for future health and disease assessments.

  5. Plasma biochemistry values of recently wild-caught purple mouth moray eels (Gymnothorax vicinus).

    Science.gov (United States)

    Erlacher-Reid, Claire; Hoffman, Walter E; Priede, Megan; Pulver, Robert; Tuttle, Allison D

    2011-12-01

    The primary purpose of this study was to establish plasma biochemistry parameters for healthy recently wild-caught purple mouth moray eels (Gymnothorax vicinus) to provide a baseline of data for improved medical care in an aquarium or zoologic setting and for wild health assessments. Thirty-one clinically healthy purple mouth moray eels of unknown age and sex were caught from the wild, and were anesthetized 50 days following capture for blood collection from the ventral coccygeal vein. The median plasma biochemistry values were as follows: hematocrit = 21%, creatinine kinase = 2,100 U/L, lactate dehydrogenase = 97 U/L, aspartate aminotransferase = 88 U/L, alanine aminotransferase = 51 U/L, alkaline phosphatase 3,939 U/L, gamma-glutamyl transpeptidase = 1 U/L, amylase = 40 U/L, blood urea nitrogen = moray eels to aid in monitoring elevations to these values in an aquarium setting over time so adjustments to the dietary regime may be utilized to prevent or improve conditions such as lipid keratopathy.

  6. FT-IR Characterization of Pollen Biochemistry, Viability, and Germination Capacity in Saintpaulia H. Wendl. Genotypes

    Directory of Open Access Journals (Sweden)

    Erzsebet Buta

    2015-01-01

    Full Text Available FT-IR characterization of pollen biochemistry was analyzed to detect possible connection with the viability (by staining with potassium iodide, 25% and the germination capacity (on solid nutrient medium, in 15 Saintpaulia genotypes. Vibrational spectroscopy indicates that the pollen of S. ionantha genotype “Red Velvet” is rich in proteins, lipids, triglycerides, and esters and has a viability of 88.4% and a low germination capacity (27.16%. For S. ionantha “Jolly Red” and “Lucky Ladybug” genotypes, pollen showed high viability (88.81–91.49% and low germination capacity (23.02–9.17%, even though the pollen is rich in carbohydrates. S. ionantha “Aloha Orchid” genotype has the highest percentage of viability (94.32% and germination capacity (45.73% and a rich content of carbohydrates and polygalacturonic acids. In S. rupicola and S. ionantha genotypes, the rich content of polygalacturonic acids, lipids, and carbohydrates favourably influenced the germination capacity. Spectroscopic result indicates, through different absorbance band intensity, a possible link between biochemical composition, viability, and germination capacity of Saintpaulia pollen. To determine exactly the relation between biochemistry and biological processes, it is necessary to initiate quantitative researches.

  7. 实验室信息管理系统在四川省跨界断面水质资金扣缴监测工作中的应用%Application of Laboratory Information Management System in Ecological Compensation Water Monitoring of Funds Withholding for Cross -section Surface Water Quality of Min and Tuo Rivers in Sichuan Province

    Institute of Scientific and Technical Information of China (English)

    鲁朝旭

    2014-01-01

    This article introduces the construction goal ,construction process and operation condition of laboratory information management system (LIMS) in ecological compensation water monitoring of funds withholding for cross -section surface water quality of Min and Tuo Rivers in Sichuan Province .On the basis of practice in the last two years ,it puts forward some suggestions for the construction of LIMS in environmental monitoring field .%介绍了四川省跨界断面水质资金扣缴工作环境监测实验室信息管理系统( LIMS)的构建目标、建设过程以及运行情况,在近两年的实际应用基础上,提出了实验室信息管理系统在环境监测领域建立的几点建议。

  8. Biochemistry in the High School Textbook: A Distance Between Content And Students Reality?

    Directory of Open Access Journals (Sweden)

    A. Simões-Pires

    2012-05-01

    Full Text Available Several  contents  have  been  taught  in  a  decontextualized  way  within  the  area  of biology,  one  of  these  contents  is  Biochemistry.  Biochemistry  is  one  of  the  most present subjects in the student’s daily lives, because it can explain a huge number of  processes  that  happens  in  their  routine.  The  textbook  is  the  main  tool  in  the Brazilian  public  high  schools  classrooms,  so  a  huge  number  of  programs  to improve the textbooks were created by the government, like the National Program of the Textbook or PNLD( translated from the Portuguese ‘’Programa Nacional do Livro Didático’’. But despite the efforts to improve the textbook, their quality is still questionable, for such reasons like the distance between contents showed in the textbook and student’s reality. The main objective of this work is to evaluate how the  high  school  textbooks  show  the  content  related  to  biochemistry  and  how  this content is contextualized in the student’s reality.  To achieve this goal we analyzed four  textbooks  that  are  most  frequently  used  in  the  schools  today  and  evaluated parameters  related  with  contextualization  between  the  biochemistry  content  and students  daily  lives  situations.  The  analysis  was  done  on  a  qualitative  way.  We observed  a  lack  of  daily  examples  in  the  biochemical  contents,  an  excess  of scientific terms without clear explanations and also a huge presence of conceptual errors  in  all  four  textbooks.  The  data  showed  here  leads  us  to  conclude  that  the science’s textbooks analyzed in this study are inappropriate to teach biochemistry in an interesting way for the students.

  9. 46 CFR 160.060-9 - Recognized laboratory.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Recognized laboratory. 160.060-9 Section 160.060-9..., Adult and Child § 160.060-9 Recognized laboratory. (a) A manufacturer seeking Coast Guard approval of a... shall apply for approval directly to a recognized independent laboratory. The following laboratories...

  10. 7 CFR 996.22 - USDA-approved laboratory.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false USDA-approved laboratory. 996.22 Section 996.22 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... laboratory. USDA-approved laboratory means laboratories approved by the Science and Technology...

  11. 46 CFR 160.077-9 - Recognized laboratory.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Recognized laboratory. 160.077-9 Section 160.077-9... Recognized laboratory. (a) A manufacturer seeking Coast Guard approval of a product under this subpart shall... to a recognized independent laboratory. The following laboratories are recognized under §...

  12. 46 CFR 160.049-8 - Recognized laboratory.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Recognized laboratory. 160.049-8 Section 160.049-8... Recognized laboratory. (a) A manufacturer seeking Coast Guard approval of a product under this subpart shall... to a recognized independent laboratory. The following laboratories are recognized under §...

  13. 46 CFR 160.052-9 - Recognized laboratory.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Recognized laboratory. 160.052-9 Section 160.052-9..., Adult and Child § 160.052-9 Recognized laboratory. (a) A manufacturer seeking Coast Guard approval of a... shall apply for approval directly to a recognized independent laboratory. The following laboratories...

  14. 7 CFR 91.16 - Order of a laboratory service.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Order of a laboratory service. 91.16 Section 91.16..., Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) COMMODITY LABORATORY TESTING PROGRAMS SERVICES AND GENERAL INFORMATION Laboratory Service § 91.16 Order of a laboratory service....

  15. 46 CFR 160.047-7 - Recognized laboratory.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Recognized laboratory. 160.047-7 Section 160.047-7... and Child § 160.047-7 Recognized laboratory. (a) A manufacturer seeking Coast Guard approval of a... shall apply for approval directly to a recognized independent laboratory. The following laboratories...

  16. 7 CFR 91.15 - Basis of a laboratory service.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Basis of a laboratory service. 91.15 Section 91.15..., Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) COMMODITY LABORATORY TESTING PROGRAMS SERVICES AND GENERAL INFORMATION Laboratory Service § 91.15 Basis of a laboratory service....

  17. 7 CFR 91.17 - Postponing a laboratory service.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Postponing a laboratory service. 91.17 Section 91.17..., Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) COMMODITY LABORATORY TESTING PROGRAMS SERVICES AND GENERAL INFORMATION Laboratory Service § 91.17 Postponing a laboratory service. If...

  18. 46 CFR 164.019-17 - Recognized laboratory.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Recognized laboratory. 164.019-17 Section 164.019-17...: SPECIFICATIONS AND APPROVAL MATERIALS Personal Flotation Device Components § 164.019-17 Recognized laboratory. (a) General. A laboratory may be designated as a recognized laboratory under this subpart if it is—...

  19. 9 CFR 391.4 - Laboratory services rate.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Laboratory services rate. 391.4 Section 391.4 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... AND LABORATORY ACCREDITATION § 391.4 Laboratory services rate. The rate for laboratory...

  20. 46 CFR 162.039-5 - Recognized laboratory.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Recognized laboratory. 162.039-5 Section 162.039-5... Recognized laboratory. (a) A recognized laboratory is one which is regularly engaged in the examination... motorboats. The following laboratories are recognized, and the semiportable fire extinguishers bearing...