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Sample records for binds flexible gc-rich

  1. Stabilization of Oncostatin-M mRNA by Binding of Nucleolin to a GC-Rich Element in Its 3'UTR.

    Science.gov (United States)

    Saha, Sucharita; Chakraborty, Alina; Bandyopadhyay, Sumita Sengupta

    2016-04-01

    Oncostatin-M (OSM) is a patho-physiologically important pleiotropic, multifunctional cytokine. OSM mRNA sequence analysis revealed that its 3'UTR contains three highly conserved GC-rich cis-elements (GCREs) whose role in mRNA stability is unidentified. In the present study, the functional role of the proximal GC-rich region of osm 3'-UTR (GCRE-1) in post-transcriptional regulation of osm expression in U937 cells was assessed by transfecting construct containing GCRE-1 at 3'-end of a fairly stable reporter gene followed by analysis of the expression of the reporter. GCRE-1 showed mRNA destabilizing activity; however, upon PMA treatment the reporter message containing GCRE-1 was stabilized. This stabilization is owing to a time-dependent progressive binding of trans-factors (at least five proteins) to GCRE-1 post-PMA treatment. Nucleolin was identified as one of the proteins in the RNP complex of GCRE-1 with PMA-treated U937 cytosolic extracts by oligo-dT affinity chromatography of poly-adenylated GCRE-1. Immuno-blot revealed time-dependent enhancement of nucleolin in the cytoplasm which in turn directly binds GCRE-1. RNA co-immunoprecipitation confirmed the GCRE-1-nucleolin interaction in vivo. To elucidate the functional role of nucleolin in stabilization of osm mRNA, nucleolin was overexpressed in U937 cells and found to stabilize the intrinsic osm mRNA, where co-transfection with the reporter containing GCRE-1 confirms the role of GCRE-1 in stabilization of the reporter mRNA. Thus, in conclusion, the results asserted that PMA treatment in U937 cells leads to cytoplasmic translocation of nucleolin that directly binds GCRE-1, one of the major GC-rich instability elements, thereby stabilizing the osm mRNA. PMID:26399567

  2. Genome-wide chromatin remodeling identified at GC-rich long nucleosome-free regions.

    Directory of Open Access Journals (Sweden)

    Karin Schwarzbauer

    Full Text Available To gain deeper insights into principles of cell biology, it is essential to understand how cells reorganize their genomes by chromatin remodeling. We analyzed chromatin remodeling on next generation sequencing data from resting and activated T cells to determine a whole-genome chromatin remodeling landscape. We consider chromatin remodeling in terms of nucleosome repositioning which can be observed most robustly in long nucleosome-free regions (LNFRs that are occupied by nucleosomes in another cell state. We found that LNFR sequences are either AT-rich or GC-rich, where nucleosome repositioning was observed much more prominently in GC-rich LNFRs - a considerable proportion of them outside promoter regions. Using support vector machines with string kernels, we identified a GC-rich DNA sequence pattern indicating loci of nucleosome repositioning in resting T cells. This pattern appears to be also typical for CpG islands. We found out that nucleosome repositioning in GC-rich LNFRs is indeed associated with CpG islands and with binding sites of the CpG-island-binding ZF-CXXC proteins KDM2A and CFP1. That this association occurs prominently inside and also prominently outside of promoter regions hints at a mechanism governing nucleosome repositioning that acts on a whole-genome scale.

  3. A Fundamental Study of the PCR Amplification of GC-Rich DNA Templates

    OpenAIRE

    Mammedov, TG.; Pienaar, E; Whitney, SE; TerMaat, JR.; Carvill, G.; Goliath, R; Subramanian, A.; Viljoen, HJ

    2008-01-01

    A theoretical analysis is presented with experimental confirmation to conclusively demonstrate the critical role that annealing plays in efficient PCR amplification of GC-rich templates. The analysis is focused on the annealing of primers at alternative binding sites (competitive annealing) and the main result is a quantitative expression of the efficiency (η) of annealing as a function of temperature (TA), annealing period tA) and template composition. The optimal efficiency lies in a narrow...

  4. A new RACE method for extremely GC-rich genes

    OpenAIRE

    Shi, Xianzong; Jarvis, Donald L.

    2006-01-01

    Rapid amplification of cDNA ends (RACE) is widely utilized to determine the 5′- and 3′-terminal nucleotide sequences of genes. Many different RACE methods have been developed to meet various requirements, but none address the difficult problems that arise when trying to isolate the ends of extremely GC-rich genes. In this study, we found that we were unable to isolate the correct 5′ or 3′ ends of an insect gene, which appeared to include extremely GC-rich sequences, using current RACE methods...

  5. The flexible loop L1 of the H3K4 demethylase JARID1B ARID domain has a crucial role in DNA-binding activity

    International Nuclear Information System (INIS)

    JARID1B, a member of the JmjC demethylase family, has a crucial role in H3K4me3 demethylation. The ARID domain is a potential DNA-binding domain of JARID1B. Previous studies indicate that a GC-rich DNA motif is the specific target of the ARID domain. However, the details of the interaction between the ARID domain and duplex DNA require further study. Here, we utilized NMR spectroscopy to assign the backbone amino acids and mapped the DNA-binding sites of the human JARID1B ARID domain. Perturbations to 1H-15N correlation spectra revealed that the flexible loop L1 of ARID was the main DNA-binding interface. EMSA and intrinsic fluorescence experiments demonstrated that mutations on loop L1 strongly reduced the DNA-binding activity of JARID1B ARID. Furthermore, transfection of mutant forms resulted in a distinct loss of intrinsic H3K4 demethylase activity, implying that the flexible loop L1 made a major contribution to sustaining the DNA-binding ability of JARID1B ARID domain.

  6. Polymerase chain reaction amplification of a GC rich region by adding 1,2 propanediol

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    Zeinab Mousavian

    2014-01-01

    Full Text Available Background : Apolipoprotein E (ApoE is one of the most important carriers of lipids in mammalians. The gene for this lipoprotein (ApoE is located on chromosome 19 which is related with the pathogenesis of some nervous system disease. ApoE gene is identified as a high guanine-cytosine (GC content fragment. Detection and amplification of these templates are extensively laborious and baffling. The aim of this study was to find a practical and feasible method for the amplification of the number of GC rich genes such as ApoE. Materials and Methods: We experimented with simple polymerase chain reaction (PCR, nested PCR and PCR with 1-2 propanediol, dimethylsulfoxide (DMSO, and ethyleneglicol as additive substances to enhance the amplification ApoE gene and used the 40 samples of the human whole blood were collected in test tubes with a pre-treatment of ethylene diaminetetraacetic acid. Results: According to our observations, presence of 1-2 propanediol, DMSO, and ethyleneglicol as additive substances resulted to enhanced amplification of ApoE gene. Addition of 1-2 propanediol showed the best results, caused optimization and revealed more specific and sharp bands. Conclusion: According to our findings 1-2 propanediol are the best organic reagent for improving the amplification of ApoE gene. Optimization procedure for each GC rich sequence is recommended to be performed separately in order to identify which of the additive agent is more efficient and applicable for a particular target.

  7. Molecular Cytogenetics in Trough Shells (Mactridae, Bivalvia): Divergent GC-Rich Heterochromatin Content.

    Science.gov (United States)

    García-Souto, Daniel; Pérez-García, Concepción; Kendall, Jack; Pasantes, Juan J

    2016-01-01

    The family Mactridae is composed of a diverse group of marine organisms, commonly known as trough shells or surf clams, which illustrate a global distribution. Although this family includes some of the most fished and cultured bivalve species, their chromosomes are poorly studied. In this work, we analyzed the chromosomes of Spisula solida, Spisula subtruncata and Mactra stultorum by means of fluorochrome staining, C-banding and fluorescent in situ hybridization using 28S ribosomal DNA (rDNA), 5S rDNA, H3 histone gene and telomeric probes. All three trough shells presented 2n = 38 chromosomes but different karyotype compositions. As happens in most bivalves, GC-rich regions were limited to the nucleolus organizing regions in Spisula solida. In contrast, many GC-rich heterochromatic bands were detected in both Spisula subtruncata and Mactra stultorum. Although the three trough shells presented single 5S rDNA and H3 histone gene clusters, their chromosomal locations differed. Regarding major rDNA clusters, while Spisula subtruncata presented a single cluster, both Spisula solida and Mactra stultorum showed two. No evidence of intercalary telomeric signals was detected in these species. The molecular cytogenetic characterization of these taxa will contribute to understanding the role played by chromosome changes in the evolution of trough shells. PMID:27537915

  8. An efficient rRNA removal method for RNA sequencing in GC-rich bacteria

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    Peano Clelia

    2013-01-01

    Full Text Available Abstract Background Next generation sequencing (NGS technologies have revolutionized gene expression studies and functional genomics analysis. However, further improvement of RNA sequencing protocols is still desirable, in order to reduce NGS costs and to increase its accuracy. In bacteria, a major problem in RNA sequencing is the abundance of ribosomal RNA (rRNA, which accounts for 95-98% of total RNA and can therefore hinder sufficient coverage of mRNA, the main focus of transcriptomic studies. Thus, efficient removal of rRNA is necessary to achieve optimal coverage, good detection sensitivity and reliable results. An additional challenge is presented by microorganisms with GC-rich genomes, in which rRNA removal is less efficient. Results In this work, we tested two commercial kits for rRNA removal, either alone or in combination, on Burkholderia thailandensis. This bacterium, chosen as representative of the important Burkholderia genus, which includes both pathogenic and environmental bacteria, has a rather large (6.72 Mb and GC-rich (67.7% genome. Each enriched mRNA sample was sequenced through paired-end Illumina GAIIx run in duplicate, yielding between 10 and 40 million reads. We show that combined treatment with both kits allows an mRNA enrichment of more than 238-fold, enabling the sequencing of almost all (more than 90% B. thailandensis transcripts from less than 10 million reads, without introducing any bias in mRNA relative abundance, thus preserving differential expression profile. Conclusions The mRNA enrichment protocol presented in this work leads to an increase in detection sensitivity up to 770% compared to total RNA; such increased sensitivity allows for a corresponding reduction in the number of sequencing reads necessary for the complete analysis of whole transcriptome expression profiling. Thus we can conclude that the MICROBExpress/Ovation combined rRNA removal method could be suitable for RNA sequencing of whole

  9. Conformational flexibility of avidin: the influence of biotin binding

    International Nuclear Information System (INIS)

    Ligand binding to proteins is a key process in cell biochemistry. The interaction usually induces modifications in the unfolding thermodynamic parameters of the macromolecule due to the coupling of unfolding and binding equilibria. In addition, these modifications can be attended by changes in protein structure and/or conformational flexibility induced by ligand binding. In this work, we have explored the effect of biotin binding on conformation and dynamic properties of avidin by using infrared spectroscopy including kinetics of hydrogen/deuterium exchange. Our results, along with previously thermodynamic published data, indicate a clear correlation between thermostability and protein compactness. In addition, our results also help to interpret the thermodynamic binding parameters of the exceptionally stable biotin:AVD complex

  10. GC-Rich Extracellular DNA Induces Oxidative Stress, Double-Strand DNA Breaks, and DNA Damage Response in Human Adipose-Derived Mesenchymal Stem Cells

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    Svetlana Kostyuk

    2015-01-01

    Full Text Available Background. Cell free DNA (cfDNA circulates throughout the bloodstream of both healthy people and patients with various diseases. CfDNA is substantially enriched in its GC-content as compared with human genomic DNA. Principal Findings. Exposure of haMSCs to GC-DNA induces short-term oxidative stress (determined with H2DCFH-DA and results in both single- and double-strand DNA breaks (comet assay and γH2AX, foci. As a result in the cells significantly increases the expression of repair genes (BRCA1 (RT-PCR, PCNA (FACS and antiapoptotic genes (BCL2 (RT-PCR and FACS, BCL2A1, BCL2L1, BIRC3, and BIRC2 (RT-PCR. Under the action of GC-DNA the potential of mitochondria was increased. Here we show that GC-rich extracellular DNA stimulates adipocyte differentiation of human adipose-derived mesenchymal stem cells (haMSCs. Exposure to GC-DNA leads to an increase in the level of RNAPPARG2 and LPL (RT-PCR, in the level of fatty acid binding protein FABP4 (FACS analysis and in the level of fat (Oil Red O. Conclusions. GC-rich fragments in the pool of cfDNA can potentially induce oxidative stress and DNA damage response and affect the direction of mesenchymal stem cells differentiation in human adipose—derived mesenchymal stem cells. Such a response may be one of the causes of obesity or osteoporosis.

  11. Exploring Hydrophobic Binding Surfaces Using Comfa and Flexible Hydrophobic Ligands

    Science.gov (United States)

    Thakkar, Shraddha; Sanchez, Rosa. I.; Bhuveneswaran, Chidambaram; Compadre, Cesar M.

    2011-06-01

    Cysteine proteinases are a very important group of enzymes involved in a variety of physiological and pathological processes including cancer metastasis and rheumatoid arthritis. In this investigation we used 3D-Quantitative Structure Activity Relationships (3D-QSAR) techniques to model the binding of a variety of substrates to two cysteine proteinases, papain, and cathepsin B. The analysis was performed using Comparative Molecular Field Analysis (CoMFA). The molecules were constructed using standard bond angles and lengths, minimized and aligned. Charges were calculated using the PM3 method in MOPAC. The CoMFA models derived for the binding of the studied substrates to the two proteinases were compared with the expected results from the experimental X-ray crystal structures of the same proteinases. The results showed the value of CoMFA modeling of flexible hydrophobic ligands to analyze ligand binding to protein receptors, and could also serve as the basis to design specific inhibitors of cysteine proteinases with potential therapeutic value.

  12. Complete Genome Sequence of Elephant Endotheliotropic Herpesvirus 4, the First Example of a GC-Rich Branch Proboscivirus

    Science.gov (United States)

    Ling, Paul D.; Long, Simon Y.; Fuery, Angela; Peng, Rong-Sheng; Heaggans, Sarah Y.; Qin, Xiang; Worley, Kim C.; Dugan, Shannon

    2016-01-01

    ABSTRACT A novel group of mammalian DNA viruses called elephant endotheliotropic herpesviruses (EEHVs) belonging to the Proboscivirus genus has been associated with nearly 100 cases of highly lethal acute hemorrhagic disease in young Asian elephants worldwide. The complete 180-kb genomes of prototype strains from three AT-rich branch viruses, EEHV1A, EEHV1B, and EEHV5, have been published. However, less than 6 kb of DNA sequence each from EEHV3, EEHV4, and EEHV7 showed them to be a hugely diverged second major branch with GC-rich characteristics. Here, we determined the complete 206-kb genome of EEHV4(Baylor) directly from trunk wash DNA by next-generation sequencing and de novo assembly procedures. Among a total of 119 genes with an overall colinear organization similar to those of the AT-rich EEHVs, major features of EEHV4 include a family of 26 paralogous 7xTM and vGPCR-like genes plus 25 novel or missing genes. The genome also contains an unusual distribution of tracts of 5 to 11 successive A or T nucleotides in intergenic domains between the mostly much higher GC content protein coding regions. Furthermore, an extremely high GC-rich bias in the third wobble position of codons clearly delineates the coding regions for many but not all proteins. There are also two novel captured cellular genes, including a C-type lectin (vECTL) and an O-linked acetylglucosamine transferase (vOGT), as well as an unusually large and complex Ori-Lyt dyad symmetry domain. Finally, 30 kb from a second strain proved to include three small chimeric domains, indicating the existence of distinct EEHV4A and EEHV4B subtypes. IMPORTANCE Multiple species of herpesviruses from three different lineages of the Proboscivirus genus (EEHV1/6, EEHV2/5, and EEHV3/4/7) infect both Asian and African elephants, but lethal hemorrhagic disease is largely confined to Asian elephant calves and is predominantly associated with EEHV1. Milder disease caused by EEHV5 or EEHV4 is being increasingly recognized

  13. The GC-Rich Mitochondrial and Plastid Genomes of the Green Alga Coccomyxa Give Insight into the Evolution of Organelle DNA Nucleotide Landscape

    Energy Technology Data Exchange (ETDEWEB)

    Smith, David Roy; Burki, Fabien; Yamada, Takashi; Grimwood, Jane; Grigoriev, Igor V.; Van Etten, James L.; Keeling, Patrick J.

    2011-05-13

    Most of the available mitochondrial and plastid genome sequences are biased towards adenine and thymine (AT) over guanine and cytosine (GC). Examples of GC-rich organelle DNAs are limited to a small but eclectic list of species, including certain green algae. Here, to gain insight in the evolution of organelle nucleotide landscape, we present the GC-rich mitochondrial and plastid DNAs from the trebouxiophyte green alga Coccomyxa sp. C-169. We compare these sequences with other GC-rich organelle DNAs and argue that the forces biasing them towards G and C are nonadaptive and linked to the metabolic and/or life history features of this species. The Coccomyxa organelle genomes are also used for phylogenetic analyses, which highlight the complexities in trying to resolve the interrelationships among the core chlorophyte green algae, but ultimately favour a sister relationship between the Ulvophyceae and Chlorophyceae, with the Trebouxiophyceae branching at the base of the chlorophyte crown.

  14. Flexible protein-peptide docking using CABS-dock with knowledge about the binding site

    OpenAIRE

    Kurcinski, Mateusz; Ciemny, Maciej Pawel; Blaszczyk, Maciej; Kolinski, Andrzej; Kmiecik, Sebastian

    2016-01-01

    Despite considerable efforts, structural prediction of protein-peptide complexes is still a very challenging task, mainly due to two reasons: high flexibility of the peptides and transient character of their interactions with proteins. Recently we have developed an automated web server CABS-dock (http://biocomp.chem.uw.edu.pl/CABSdock), which conducts flexible protein-peptide docking without any knowledge about the binding site. Our method allows for full flexibility of the peptide, whereas t...

  15. Differential utilization of binding loop flexibility in T cell receptor ligand selection and cross-reactivity.

    Science.gov (United States)

    Ayres, Cory M; Scott, Daniel R; Corcelli, Steven A; Baker, Brian M

    2016-01-01

    Complementarity determining region (CDR) loop flexibility has been suggested to play an important role in the selection and binding of ligands by T cell receptors (TCRs) of the cellular immune system. However, questions remain regarding the role of loop motion in TCR binding, and crystallographic structures have raised questions about the extent to which generalizations can be made. Here we studied the flexibility of two structurally well characterized αβ TCRs, A6 and DMF5. We found that the two receptors utilize loop motion very differently in ligand binding and cross-reactivity. While the loops of A6 move rapidly in an uncorrelated fashion, those of DMF5 are substantially less mobile. Accordingly, the mechanisms of binding and cross-reactivity are very different between the two TCRs: whereas A6 relies on conformational selection to select and bind different ligands, DMF5 uses a more rigid, permissive architecture with greater reliance on slower motions or induced-fit. In addition to binding site flexibility, we also explored whether ligand-binding resulted in common dynamical changes in A6 and DMF5 that could contribute to TCR triggering. Although binding-linked motional changes propagated throughout both receptors, no common features were observed, suggesting that changes in nanosecond-level TCR structural dynamics do not contribute to T cell signaling. PMID:27118724

  16. AutoDockFR: Advances in Protein-Ligand Docking with Explicitly Specified Binding Site Flexibility

    OpenAIRE

    Ravindranath, Pradeep Anand; Forli, Stefano; Goodsell, David S.; Olson, Arthur J.; Sanner, Michel F.

    2015-01-01

    Automated docking of drug-like molecules into receptors is an essential tool in structure-based drug design. While modeling receptor flexibility is important for correctly predicting ligand binding, it still remains challenging. This work focuses on an approach in which receptor flexibility is modeled by explicitly specifying a set of receptor side-chains a-priori. The challenges of this approach include the: 1) exponential growth of the search space, demanding more efficient search methods; ...

  17. Comparison of the Gene Coding Contents and Other Unusual Features of the GC-Rich and AT-Rich Branch Probosciviruses.

    Science.gov (United States)

    Ling, Paul D; Long, Simon Y; Zong, Jian-Chao; Heaggans, Sarah Y; Qin, Xiang; Hayward, Gary S

    2016-01-01

    Nearly 100 cases of lethal acute hemorrhagic disease in young Asian elephants have been reported worldwide. All tested cases contained high levels of elephant endotheliotropic herpesvirus (EEHV) DNA in pathological blood or tissue samples. Seven known major types of EEHVs have been partially characterized and shown to all belong to the novel Proboscivirus genus. However, the recently determined 206-kb EEHV4 genome proved to represent the prototype of a GC-rich branch virus that is very distinct from the previously published 180-kb EEHV1A, EEHV1B, and EEHV5A genomes, which all fall within an alternative AT-rich branch. Although EEHV4 retains the large family of 7xTM and vGPCR-like genes, six are unique to either just one or the other branch. While both branches display a highly enriched distribution of A and T tracts in intergenic domains, they are generally much larger within the GC-rich branch. Both branches retain the vGCNT1 acetylglucosamine transferase and at least one vOX-2 gene, but the two branches differ by 25 genes overall, with the AT-rich branch encoding a fucosyl transferase (vFUT9) plus two or three more vOX2 proteins and an immunoglobulin-like gene family that are all absent from the GC-rich branch. Several envelope glycoproteins retain only 15 to 20% protein identity or less across the two branches. Finally, the two plausible predicted transcriptional regulatory proteins display no homology at all to those in the alpha-, beta-, or gammaherpesvirus subfamilies. These results reinforce our previous proposal that the probosciviruses should be designated a new subfamily of mammalian herpesviruses. IMPORTANCE Multiple species of herpesviruses from three different lineages of the Proboscivirus genus (EEHV1/6, EEHV2/5, and EEHV3/4/7) infect either Asian or African elephants, but the highly lethal hemorrhagic disease is largely confined to Asian elephant calves and is predominantly associated with EEHV1. In the accompanying paper [P. D. Ling et al., m

  18. Structural aspects of the interaction of anticancer drug Actinomycin-D to the GC rich region of hmgb1 gene.

    Science.gov (United States)

    Lohani, Neelam; Singh, Himanshu Narayan; Moganty, R Rajeswari

    2016-06-01

    The high mobility group box 1 protein has been identified as a key player in chromatin homeostasis including transcription regulation, recombination, repair, and chromatin remodeling. Emerging findings indicate HMGB1 protein over expression in nearly all types of human cancers and inflammatory disorders. Thus it is considered as a potential therapeutic target for treating various malignancies. We screened the promoter region of hmgb1 gene and selected a positive regulatory element of 25 base pair duplex (25RY) (-165 to -183) as a potential target for chemotherapeutic intervention. The molecular interaction of actinomycin (ACT) with the regulatory region of hmgb1 gene was characterized by spectroscopic, calorimetric and molecular docking studies. The hypochromic and bathochromic shift in the absorption spectrum, stabilization of 25RY duplex against thermal denaturation, perturbation of CD spectrum of duplex and enhancement of fluorescence intensity of actinomycin indicate strong binding of actinomycin to the hmgb1 promoter region (25RY).The energetics was characterized to be endothermic and entropy driven. All these results are in good agreement with in silico investigation that suggest minor groove binding with effective intercalation at GC bases of actinomycin to 25RY. This study identifies hmgb1 gene promoter region a potential target for the anticancer therapautiucs. PMID:26923673

  19. Ultrafast differential flexibility of Cro-protein binding domains of two operator DNAs with different sequences.

    Science.gov (United States)

    Choudhury, Susobhan; Ghosh, Basusree; Singh, Priya; Ghosh, Raka; Roy, Siddhartha; Pal, Samir Kumar

    2016-07-21

    The nature of the interface of specific protein-DNA complexes has attracted immense interest in contemporary molecular biology. Although extensive studies on the role of flexibility of DNA in the specific interaction in the genetic regulatory activity of lambda Cro (Cro-protein) have been performed, the exploration of quantitative features remains deficient. In this study, we have mutated (site directed mutagenesis: SDM) Cro-protein at the 37th position with a cysteine residue (G37C) retaining the functional integrity of the protein and labelled the cysteine residue, which is close to the interface, with a fluorescent probe (AEDANS), for the investigation of its interface with operator DNAs (OR3 and OR2). We have employed picosecond resolved polarization gated fluorescence spectroscopy and the well known strategy of solvation dynamics for the exploration of physical motions of the fluorescent probes and associated environments, respectively. Even though this particular probe on the protein (AEDANS) shows marginal changes in its structural flexibility upon interaction with the DNAs, a non-covalent DNA bound probe (DAPI), which binds to the minor groove, shows a major differential alteration in the dynamical flexibility in the OR3-Cro complex when compared to that of the OR2 complex with the Cro-protein. We attempt to correlate the observed significant structural fluctuation of the Cro-protein binding domain of OR3 for the specificity of the protein to the operator DNA. PMID:27326896

  20. AutoDockFR: Advances in Protein-Ligand Docking with Explicitly Specified Binding Site Flexibility.

    Science.gov (United States)

    Ravindranath, Pradeep Anand; Forli, Stefano; Goodsell, David S; Olson, Arthur J; Sanner, Michel F

    2015-12-01

    Automated docking of drug-like molecules into receptors is an essential tool in structure-based drug design. While modeling receptor flexibility is important for correctly predicting ligand binding, it still remains challenging. This work focuses on an approach in which receptor flexibility is modeled by explicitly specifying a set of receptor side-chains a-priori. The challenges of this approach include the: 1) exponential growth of the search space, demanding more efficient search methods; and 2) increased number of false positives, calling for scoring functions tailored for flexible receptor docking. We present AutoDockFR-AutoDock for Flexible Receptors (ADFR), a new docking engine based on the AutoDock4 scoring function, which addresses the aforementioned challenges with a new Genetic Algorithm (GA) and customized scoring function. We validate ADFR using the Astex Diverse Set, demonstrating an increase in efficiency and reliability of its GA over the one implemented in AutoDock4. We demonstrate greatly increased success rates when cross-docking ligands into apo receptors that require side-chain conformational changes for ligand binding. These cross-docking experiments are based on two datasets: 1) SEQ17 -a receptor diversity set containing 17 pairs of apo-holo structures; and 2) CDK2 -a ligand diversity set composed of one CDK2 apo structure and 52 known bound inhibitors. We show that, when cross-docking ligands into the apo conformation of the receptors with up to 14 flexible side-chains, ADFR reports more correctly cross-docked ligands than AutoDock Vina on both datasets with solutions found for 70.6% vs. 35.3% systems on SEQ17, and 76.9% vs. 61.5% on CDK2. ADFR also outperforms AutoDock Vina in number of top ranking solutions on both datasets. Furthermore, we show that correctly docked CDK2 complexes re-create on average 79.8% of all pairwise atomic interactions between the ligand and moving receptor atoms in the holo complexes. Finally, we show that down

  1. AutoDockFR: Advances in Protein-Ligand Docking with Explicitly Specified Binding Site Flexibility.

    Directory of Open Access Journals (Sweden)

    Pradeep Anand Ravindranath

    2015-12-01

    Full Text Available Automated docking of drug-like molecules into receptors is an essential tool in structure-based drug design. While modeling receptor flexibility is important for correctly predicting ligand binding, it still remains challenging. This work focuses on an approach in which receptor flexibility is modeled by explicitly specifying a set of receptor side-chains a-priori. The challenges of this approach include the: 1 exponential growth of the search space, demanding more efficient search methods; and 2 increased number of false positives, calling for scoring functions tailored for flexible receptor docking. We present AutoDockFR-AutoDock for Flexible Receptors (ADFR, a new docking engine based on the AutoDock4 scoring function, which addresses the aforementioned challenges with a new Genetic Algorithm (GA and customized scoring function. We validate ADFR using the Astex Diverse Set, demonstrating an increase in efficiency and reliability of its GA over the one implemented in AutoDock4. We demonstrate greatly increased success rates when cross-docking ligands into apo receptors that require side-chain conformational changes for ligand binding. These cross-docking experiments are based on two datasets: 1 SEQ17 -a receptor diversity set containing 17 pairs of apo-holo structures; and 2 CDK2 -a ligand diversity set composed of one CDK2 apo structure and 52 known bound inhibitors. We show that, when cross-docking ligands into the apo conformation of the receptors with up to 14 flexible side-chains, ADFR reports more correctly cross-docked ligands than AutoDock Vina on both datasets with solutions found for 70.6% vs. 35.3% systems on SEQ17, and 76.9% vs. 61.5% on CDK2. ADFR also outperforms AutoDock Vina in number of top ranking solutions on both datasets. Furthermore, we show that correctly docked CDK2 complexes re-create on average 79.8% of all pairwise atomic interactions between the ligand and moving receptor atoms in the holo complexes. Finally, we

  2. Size and molecular flexibility affect the binding of ellagitannins to bovine serum albumin.

    Science.gov (United States)

    Dobreva, Marina A; Green, Rebecca J; Mueller-Harvey, Irene; Salminen, Juha-Pekka; Howlin, Brendan J; Frazier, Richard A

    2014-09-17

    Binding to bovine serum albumin of monomeric (vescalagin and pedunculagin) and dimeric ellagitannins (roburin A, oenothein B, and gemin A) was investigated by isothermal titration calorimetry and fluorescence spectroscopy, which indicated two types of binding sites. Stronger and more specific sites exhibited affinity constants, K1, of 10(4)-10(6) M(-1) and stoichiometries, n1, of 2-13 and dominated at low tannin concentrations. Weaker and less-specific binding sites had K2 constants of 10(3)-10(5) M(-1) and stoichiometries, n2, of 16-30 and dominated at higher tannin concentrations. Binding to stronger sites appeared to be dependent on tannin flexibility and the presence of free galloyl groups. Positive entropies for all but gemin A indicated that hydrophobic interactions dominated during complexation. This was supported by an exponential relationship between the affinity, K1, and the modeled hydrophobic accessible surface area and by a linear relationship between K1 and the Stern-Volmer quenching constant, K(SV). PMID:25162485

  3. Structural, vibrational, NMR, quantum chemical, DNA binding and protein docking studies of two flexible imine oximes

    Indian Academy of Sciences (India)

    YUNUS KAYA

    2016-09-01

    Two flexible imine oxime molecules, namely, 3-(pyridin-2-ylmethylimino)-butan-2-one oxime (HL¹) and 3-(pyridin-2-ylmethylimino)-pentan-2-one oxime (HL²) have been synthesized and characterized by elemental analysis, IR and NMR techniques. The conformational behavior was investigated using the density functional theory (DFT) with the B3LYP method combined with the 6-311++G(d,p) basis set. As a result of the conformational studies, three stable molecules and the most stable conformer were determined for the both imine oximes. The spectroscopic properties such as vibrational and NMR were calculated for the most stable conformer of the HL¹ and HL². The calculation results were applied to simulate infrared spectra of the title compounds, which show good agreement with observed spectra. In addition, the stable three molecules of the both imine oximes have been used to carry out DNA binding and protein docking studies with DNA and protein structures (downloaded from Protein Data Bank) using Discovery Studio 3.5 to find the most preferred binding mode of the ligands inside the DNA and protein cavity.

  4. Stabilizing a Flexible Interdomain Hinge Region Harboring the SMB Binding Site Drives uPAR into Its Closed Conformation

    DEFF Research Database (Denmark)

    Zhao, Baoyu; Gandhi, Sonu; Yuan, Cai;

    2015-01-01

    domains is inherently flexible and binding of uPA drives uPAR into its closed conformation, which presents the higher affinity state for vitronectin thus providing an allosteric regulatory mechanism. Using a new class of epitope mapped monoclonal anti-uPAR antibodies, we now demonstrate...

  5. Crystal structure of the S. solfataricus archaeal exosome reveals conformational flexibility in the RNA-binding ring.

    Directory of Open Access Journals (Sweden)

    Changrui Lu

    Full Text Available BACKGROUND: The exosome complex is an essential RNA 3'-end processing and degradation machinery. In archaeal organisms, the exosome consists of a catalytic ring and an RNA-binding ring, both of which were previously reported to assume three-fold symmetry. METHODOLOGY/PRINCIPAL FINDINGS: Here we report an asymmetric 2.9 A Sulfolobus solfataricus archaeal exosome structure in which the three-fold symmetry is broken due to combined rigid body and thermal motions mainly within the RNA-binding ring. Since increased conformational flexibility was also observed in the RNA-binding ring of the related bacterial PNPase, we speculate that this may reflect an evolutionarily conserved mechanism to accommodate diverse RNA substrates for degradation. CONCLUSION/SIGNIFICANCE: This study clearly shows the dynamic structures within the RNA-binding domains, which provides additional insights on mechanism of asymmetric RNA binding and processing.

  6. The four Zn fingers of MBNL1 provide a flexible platform for recognition of its RNA binding elements

    Directory of Open Access Journals (Sweden)

    Gates Devika P

    2011-05-01

    Full Text Available Abstract Background Muscleblind-like 1 (MBNL1 is an alternative splicing factor containing four CCCH Zinc fingers (ZnFs. The sequestration of MBNL1 by expanded CUG and CCUG repeats is a major component in causing myotonic dystrophy. In addition to binding the structured expanded CUG and CCUG repeats; previous results suggested that MBNL1 binds single-stranded RNAs containing GC dinucleotides. Results We performed a systematic analysis of MBNL1 binding to single-stranded RNAs. These studies revealed that a single GC dinucleotide in poly-uridine is sufficient for MBNL1 binding and that a second GC dinucleotide confers higher affinity MBNL1 binding. However additional GC dinucleotides do not enhance RNA binding. We also showed that the RNA sequences adjacent to the GC dinucleotides play an important role in MBNL1 binding with the following preference: uridines >cytidines >adenosines >guanosines. For high affinity binding by MBNL1, the distance between the two GC dinucleotides can vary from 1 to 17 nucleotides. Conclusions These results suggest that MBNL1 is highly flexible and able to adopt different conformations to recognize RNAs with varying sequence configurations. Although MBNL1 contains four ZnFs, only two ZnF - GC dinucleotide interactions are necessary for high affinity binding.

  7. Size and molecular flexibility affect the binding of ellagitannins to bovine serum albumin

    OpenAIRE

    Dobreva, Marina; Green, R. J.; Mueller-Harvey, Irene; Salminen, J.-P.; Howlin, B.J.; Frazier, Richard

    2014-01-01

    Binding to bovine serum albumin of monomeric (vescalagin and pedunculagin) and dimeric ellagitannins (roburin A, oenothein B, and gemin A) was investigated by isothermal titration calorimetry and fluorescence spectroscopy, which indicated two types of binding sites. Stronger and more specific sites exhibited affinity constants, K1, of 104–106 M–1 and stoichiometries, n1, of 2–13 and dominated at low tannin concentrations. Weaker and less-specific binding sites had K2 constants of 103–105 M–1 ...

  8. Computational approaches to modeling receptor flexibility upon ligand binding: Application to interfacially activated enzymes

    DEFF Research Database (Denmark)

    Wade, R.C.; Sobolev, V.; Ortiz, A.R. .;

    1998-01-01

    Receptors generally undergo conformational change upon ligand binding. We describe how fairly simple techniques may be used in docking and design studies to account for some of the changes in the conformations of proteins on ligand binding. Simulations of protein-ligand interactions that give a m...... a more complete description of the dynamics important for ligand binding are then discussed. These methods are illustrated for phospholipase A(2) and lipase, enzymes that both undergo interfacial activation....

  9. The Fundamental Role of Flexibility on the Strength of Molecular Binding

    OpenAIRE

    Forrey, Christopher; Douglas, Jack F.; Gilson, Michael K.

    2012-01-01

    Non-covalent molecular association underlies a diverse set of biologically and technologically relevant phenomena, including the action of drugs on their biomolecular targets and self- and supra-molecular assembly processes. Computer models employed to model binding frequently use interaction potentials with atomistic detail while neglecting the thermal molecular motions of the binding species. However, errors introduced by this simplification and, more broadly, the thermodynamic consequences...

  10. Stabilizing a flexible interdomain hinge region harboring the SMB binding site drives uPAR into its closed conformation.

    Science.gov (United States)

    Zhao, Baoyu; Gandhi, Sonu; Yuan, Cai; Luo, Zhipu; Li, Rui; Gårdsvoll, Henrik; de Lorenzi, Valentina; Sidenius, Nicolai; Huang, Mingdong; Ploug, Michael

    2015-03-27

    The urokinase-type plasminogen activator receptor (uPAR) is a multidomain glycolipid-anchored membrane protein, which facilitates extracellular matrix remodeling by focalizing plasminogen activation to cell surfaces via its high-affinity interaction with uPA. The modular assembly of its three LU (Ly6/uPAR-like) domains is inherently flexible and binding of uPA drives uPAR into its closed conformation, which presents the higher-affinity state for vitronectin thus providing an allosteric regulatory mechanism. Using a new class of epitope-mapped anti-uPAR monoclonal antibodies (mAbs), we now demonstrate that the reciprocal stabilization is indeed also possible. By surface plasmon resonance studies, we show that these mAbs and vitronectin have overlapping binding sites on uPAR and that they share Arg91 as hotspot residue in their binding interfaces. The crystal structure solved for one of these uPAR·mAb complexes at 3.0Å clearly shows that this mAb preselects the closed uPAR conformation with an empty but correctly assembled large hydrophobic binding cavity for uPA. Accordingly, these mAbs inhibit the uPAR-dependent lamellipodia formation and migration on vitronectin-coated matrices irrespective of the conformational status of uPAR and its occupancy with uPA. This is the first study to the best of our knowledge, showing that the dynamic assembly of the three LU domains in uPARwt can be driven toward the closed form by an external ligand, which is not engaging the hydrophobic uPA binding cavity. As this binding interface is also exploited by the somatomedin B domain of vitronectin, therefore, this relationship should be taken into consideration when exploring uPAR-dependent cell adhesion and migration in vitronectin-rich environments. PMID:25659907

  11. Synthesis of Diphenyl Pyridazinone-based flexible system for conformational studies through weak noncovalent interactions: Application in DNA binding

    Indian Academy of Sciences (India)

    Ranjeet Kumar; Praveen Singh; Archana Gaurav; Pratima Yadav; Ranjana S Khanna; Ashish Kumar Tewari

    2016-04-01

    This paper reports conformational studies of pyridazinone-based flexible dimer connected through diethylamine linker. The conformational studies have been done by X-ray crystal structure and DFT calculation. Further, after crystallization, the compound has shown two types of crystals, one is hydrated and another one is non-hydrated. The hydrated and non-hydrated crystals showed difference in their conformation due to the presence of water in crystal lattice of hydrated crystal. The difference in their conformation has been proved by crystallographic studies, DSC curves and detailed analysis of Hirshfeld surfaces and fingerprint plots facilitating a comparison of intermolecular interactions. Along with conformational studies, this compound also showed DNA binding, as revealed in docking simulation studies.

  12. Changes in flexibility upon binding: Application of the self-consistent pair contact probability method to protein-protein interactions

    Science.gov (United States)

    Canino, Lawrence S.; Shen, Tongye; McCammon, J. Andrew

    2002-12-01

    We extend the self-consistent pair contact probability method to the evaluation of the partition function for a protein complex at thermodynamic equilibrium. Specifically, we adapt the method for multichain models and introduce a parametrization for amino acid-specific pairwise interactions. This method is similar to the Gaussian network model but allows for the adjusting of the strengths of native state contacts. The method is first validated on a high resolution x-ray crystal structure of bovine Pancreatic Phospholipase A2 by comparing calculated B-factors with reported values. We then examine binding-induced changes in flexibility in protein-protein complexes, comparing computed results with those obtained from x-ray crystal structures and molecular dynamics simulations. In particular, we focus on the mouse acetylcholinesterase:fasciculin II and the human α-thrombin:thrombomodulin complexes.

  13. Crystal structure and conformational flexibility of the unligated FK506-binding protein FKBP12.6

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Hui; Mustafi, Sourajit M. [New York State Department of Health, Empire State Plaza, Albany, NY 12201 (United States); LeMaster, David M. [New York State Department of Health, Empire State Plaza, Albany, NY 12201 (United States); University at Albany – SUNY, Empire State Plaza, Albany, NY 12201 (United States); Li, Zhong [New York State Department of Health, Empire State Plaza, Albany, NY 12201 (United States); Héroux, Annie [Brookhaven National Laboratory, Upton, NY 11973 (United States); Li, Hongmin; Hernández, Griselda, E-mail: griselda@wadsworth.org [New York State Department of Health, Empire State Plaza, Albany, NY 12201 (United States); University at Albany – SUNY, Empire State Plaza, Albany, NY 12201 (United States)

    2014-03-01

    Two crystal forms of unligated FKBP12.6 exhibit multiple conformations in the active site and in the 80s loop, the primary site for known protein-recognition interactions. The previously unreported NMR backbone assignment of FKBP12.6 revealed extensive doubling of amide resonances, which reflects a slow conformational transition centered in the 80s loop. The primary known physiological function of FKBP12.6 involves its role in regulating the RyR2 isoform of ryanodine receptor Ca{sup 2+} channels in cardiac muscle, pancreatic β islets and the central nervous system. With only a single previously reported X-ray structure of FKBP12.6, bound to the immunosuppressant rapamycin, structural inferences for this protein have been drawn from the more extensive studies of the homologous FKBP12. X-ray structures at 1.70 and 1.90 Å resolution from P2{sub 1} and P3{sub 1}21 crystal forms are reported for an unligated cysteine-free variant of FKBP12.6 which exhibit a notable diversity of conformations. In one monomer from the P3{sub 1}21 crystal form, the aromatic ring of Phe59 at the base of the active site is rotated perpendicular to its typical orientation, generating a steric conflict for the immunosuppressant-binding mode. The peptide unit linking Gly89 and Val90 at the tip of the protein-recognition ‘80s loop’ is flipped in the P2{sub 1} crystal form. Unlike the >30 reported FKBP12 structures, the backbone conformation of this loop closely follows that of the first FKBP domain of FKBP51. The NMR resonances for 21 backbone amides of FKBP12.6 are doubled, corresponding to a slow conformational transition centered near the tip of the 80s loop, as recently reported for 31 amides of FKBP12. The comparative absence of doubling for residues along the opposite face of the active-site pocket in FKBP12.6 may in part reflect attenuated structural coupling owing to increased conformational plasticity around the Phe59 ring.

  14. Crystal structure and conformational flexibility of the unligated FK506-binding protein FKBP12.6

    International Nuclear Information System (INIS)

    Two crystal forms of unligated FKBP12.6 exhibit multiple conformations in the active site and in the 80s loop, the primary site for known protein-recognition interactions. The previously unreported NMR backbone assignment of FKBP12.6 revealed extensive doubling of amide resonances, which reflects a slow conformational transition centered in the 80s loop. The primary known physiological function of FKBP12.6 involves its role in regulating the RyR2 isoform of ryanodine receptor Ca2+ channels in cardiac muscle, pancreatic β islets and the central nervous system. With only a single previously reported X-ray structure of FKBP12.6, bound to the immunosuppressant rapamycin, structural inferences for this protein have been drawn from the more extensive studies of the homologous FKBP12. X-ray structures at 1.70 and 1.90 Å resolution from P21 and P3121 crystal forms are reported for an unligated cysteine-free variant of FKBP12.6 which exhibit a notable diversity of conformations. In one monomer from the P3121 crystal form, the aromatic ring of Phe59 at the base of the active site is rotated perpendicular to its typical orientation, generating a steric conflict for the immunosuppressant-binding mode. The peptide unit linking Gly89 and Val90 at the tip of the protein-recognition ‘80s loop’ is flipped in the P21 crystal form. Unlike the >30 reported FKBP12 structures, the backbone conformation of this loop closely follows that of the first FKBP domain of FKBP51. The NMR resonances for 21 backbone amides of FKBP12.6 are doubled, corresponding to a slow conformational transition centered near the tip of the 80s loop, as recently reported for 31 amides of FKBP12. The comparative absence of doubling for residues along the opposite face of the active-site pocket in FKBP12.6 may in part reflect attenuated structural coupling owing to increased conformational plasticity around the Phe59 ring

  15. Comparative Genomic Study Reveals a Transition from TA Richness in Invertebrates to GC Richness in Vertebrates at CpG Flanking Sites: An Indication for Context-Dependent Mutagenicity of Methylated CpG Sites

    Institute of Scientific and Technical Information of China (English)

    Yong Wang; Frederick C.C. Leung

    2008-01-01

    Vertebrate genomes are characterized with CpG deficiency, particularly for GC-poor regions. The GC content-related CpG deficiency is probably caused by context-dependent deamination of methylated CpG sites. This hypothesis was examined in this study by comparing nucleotide frequencies at CpG flanking positions among invertebrate and vertebrate genomes. The finding is a transition of nucleotide preference of 5' T to 5' A at the invertebrate-vertebrate boundary, indicating that a large number of CpG sites with 5' Ts were depleted because of global DNA methylation developed in vertebrates. At genome level, we investigated CpG observed/expected (obs/exp) values in 500 bp fragments, and found that higher CpG obs/exp value is shown in GC-poor regions of invertebrate genomes (except sea urchin) but in GC-rich sequences of vertebrate genomes. We next compared GC content at CpG flanking positions with genomic average, showing that the GC content is lower than the average in invertebrate genomes, but higher than that in vertebrate genomes. These results indicate that although 5' T and 5' A are different in inducing deamination of methylated CpG sites, GC content is even more important in affecting the deamination rate. In all the tests, the results of sea urchin are similar to vertebrates perhaps due to its fractional DNA methylation.CpG deficiency is therefore suggested to be mainly a result of high mutation rates of methylated CpG sites in GC-poor regions.

  16. Flexible Acyclic Polyol-Chloride Anion Complexes and Their Characterization by Photoelectron Spectroscopy and Variable Temperature Binding Constant Determinations.

    Science.gov (United States)

    Shokri, Alireza; Wang, Xue-Bin; Wang, Yanping; O'Doherty, George A; Kass, Steven R

    2016-03-17

    Flexible acyclic alcohols with one to five hydroxyl groups were bound to a chloride anion and these complexes were interrogated by negative ion photoelectron spectroscopy and companion density functional theory computations. The resulting vertical detachment energies are reproduced on average to 0.10 eV by M06-2X/aug-cc-pVTZ predictions and range from 4.45-5.96 eV. These values are 0.84-2.35 eV larger than the adiabatic detachment energy of Cl(-) as a result of the larger hydrogen bond networks in the bigger polyols. Adiabatic detachment energies of the alcohol-Cl(-) clusters are more difficult to determine both experimentally and computationally. This is due to the large geometry changes that occur upon photodetachment and the large bond dissociation energy of H-Cl which enables the resulting chlorine atom to abstract a hydrogen from any of the methylene (CH2) or methine (CH) positions. Both ionic and nonionic hydrogen bonds (i.e., OH···Cl(-) and OH···OH···Cl(-)) form in the larger polyols complexes and are found to be energetically comparable. Subtle structural differences, consequently can lead to the formation of different types of hydrogen bonds, and maximizing the ionic ones is not always preferred. Solution equilibrium binding constants between the alcohols and tetrabutylammonium chloride (TBACl) in acetonitrile at -24.2, +22.0, and +53.6 °C were also determined. The free energies of association are nearly identical for all of the substrates (i.e., ΔG° = -2.8 ± 0.7 kcal mol(-1)). Compensating enthalpy and entropy values reveal, contrary to expectation and the intrinsic gas-phase preferences, that the bigger systems with more hydroxyl groups are entropically favored and enthalpically disfavored relative to the smaller species. This suggests that more solvent molecules are released upon binding TBACl to alcohols with more hydroxyl groups and is consistent with the measured negative heat capacities. These quantities increase with molecular

  17. Flexible Acyclic Polyol-Chloride Anion Complexes and Their Characterization by Photoelectron Spectroscopy and Variable Temperature Binding Constant Determinations

    Energy Technology Data Exchange (ETDEWEB)

    Shokri, Alireza; Wang, Xue B.; Wang, Yangping; O' Doherty, George A.; Kass, Steven R.

    2016-03-17

    Flexible acyclic alcohols with 1–5 hydroxyl groups were bound to chloride anion and these complexes were interrogated by negative ion photoelectron spectroscopy and companion density functional theory computations. The resulting vertical detachment energies are reproduced on average to 0.10 eV by M06-2X/aug-cc-pVTZ predictions and range from 4.45 – 5.96 eV. These values are 0.84 – 2.35 eV larger than the adiabatic detachment energy of Cl– as a result of the larger hydrogen bond networks in the bigger polyols. Adiabatic detachment energies of the alcohol–Cl– clusters are more difficult to determine both experimentally and computationally. This is due to the large geometry changes that occur upon photodetachment and the large bond dissociation energy of H–Cl which enables the resulting chlorine atom to abstract a hydrogen from any of the methylene (CH2) or methine (CH) positions. Both ionic and non-ionic hydrogen bonds (i.e., OH•••Cl– and OH•••OH•••Cl–) form in the larger polyols complexes, and are found to be energetically comparable. Subtle structural differences, consequently can lead to the formation of different types of hydrogen bonds and maximizing the ionic ones is not always preferred. Solution equilibrium binding constants between the alcohols and tetrrabuylammonium chloride (TBACl) in acetonitrile at -24.2, 22.0, and 53.6 °C were also determined. The free energies of association are nearly identical for all of the substrates (i.e., ΔG° = -2.8 ± 0.7 kcal mol–1). Compensating enthalpy and entropy values reveal, contrary to expectation and the intrinsic gas-phase preferences, that the bigger systems with more hydroxyl groups are entropically favored and enthalpically disfavored relative to the smaller species. This suggests that more solvent molecules are released upon binding TBACl to alcohols with more hydroxyl groups and is consistent with the measured negative heat capacities. These quantities increase with

  18. Flexibility of EF-hand motifs: structural and thermodynamic studies of Calcium Binding Protein-1 from Entamoeba histolytica with Pb2+, Ba2+, and Sr2+

    Directory of Open Access Journals (Sweden)

    Kumar Shivesh

    2012-08-01

    Full Text Available Abstract Background EF-hand proteins can be activated by the binding of various heavy metals other than calcium, and such complexes can disturb the calcium-signaling pathway and cause toxicity and disease causing state. So far, no comprehensive study has been done to understand different heavy metals binding to calcium signaling proteins. Results In this work, the flexibility of the EF-hand motifs are examined by crystallographic and thermodynamic studies of binding of Pb2+, Ba2+ and Sr2+ to Calcium Binding Protein-1 from Entamoeba histolytica (EhCaBP1. The structures of the EhCaBP1- heavy metal complexes are found to be overall similar, nevertheless specific differences in metal coordination, and small differences in the coordination distances between the metal and the ligands in the metal binding loop. The largest such distances occur for the Ba2+- EhCaBP1 complex, where two bariums are bound with partial occupancy at the EF2 motif. Thermodynamic studies confirm that EhCaBP1 has five binding sites for Ba2+ compared to four binding sites for the other metals. These structures and thermodynamic studies reveal that the EF-hand motifs can accommodate several heavy atoms with similar binding affinities. The binding of Ca2+ to the 1st, 2nd and 4th sites and the binding of Ba2+ to the 1st, 2nd, 4th and 5th sites are both enthalpically and entropically driven, whereas the binding of Sr2+ to the 1st, 2nd and 4th sites are simply enthalpy driven, interestingly in agreement with ITC data, Sr2+ do not coordinate with water in this structure. For all the metals, binding to the 3rd site is only entropy driven. Conclusion Energetically, Ca2+ is preferred in three sites, while in one site Ba2+ has better binding energy. The Sr2+-coordination in the EF hand motifs is similar to that of the native Ca2+ bound structure, except for the lack of water coordination. Sr2+ coordination seems to be a pre-formed in nature since all seven coordinating atoms are from the

  19. The Laminin 511/521 Binding Site on the Lutheran Blood Group Glycoprotein is Located at theFlexible Junction of Ig Domains 2 and 3

    Energy Technology Data Exchange (ETDEWEB)

    Mankelow, Tosti J.; Burton, Nicholas; Stedansdottir, Fanney O.; Spring, Frances A.; Parsons, Stephen F.; Pesersen, Jan S.; Oliveira, Cristiano L.P.; Lammie, Donna; Wess, Timothy; Mohandas, Narla; Chasis, Joel A.; Brady, R. Leo; Anstee, David J.

    2007-07-01

    The Lutheran blood group glycoprotein, first discovered on erythrocytes, is widely expressed in human tissues. It is a ligand for the {alpha}5 subunit of Laminin 511/521, an extracellular matrix protein. This interaction may contribute to vasocclusive events that are an important cause of morbidity in sickle cell disease. Using X-ray crystallography, small angle X-ray scattering and site directed mutagenesis we show that the extracellular region of Lutheran forms an extended structure with a distinctive bend between the second and third immunoglobulin-like domains. The linker between domains 2 and 3 appears to be flexible and is a critical determinant in maintaining an overall conformation for Lutheran that is capable of binding to Laminin. Mutagenesis studies indicate that Asp312 of Lutheran and the surrounding cluster of negatively charged residues in this linker region form the Laminin binding site. Unusually, receptor binding is therefore not a function of the domains expected to be furthermost from the plasma membrane. These studies imply that structural flexibility of Lutheran may be essential for its interaction with Laminin and present a novel opportunity for the development of therapeutics for sickle cell disease.

  20. Role of solution conformation and flexibility of short peptide ligands that bind to the p56(lck) SH2 domain

    NARCIS (Netherlands)

    Dekker, Frank J; de Mol, Nico J; Bultinck, Patrick; Kemmink, Johan; Hilbers, Hans W; Liskamp, Rob M J; Dekker, Frank

    2003-01-01

    A general approach in drug design is making ligands more rigid in order to avoid loss in conformational entropy (deltaS(conf)) upon receptor binding. We hypothesized that in the high affinity binding of pYEEI peptide ligands to the p56(lck) SH2 domain this loss in deltaS(conf) might be diminished du

  1. Functional and phylogenetic analyses of chromosome 21 promoters and hominid-specific transcription factor binding sites

    OpenAIRE

    Querfurth, Robert

    2011-01-01

    The focus of this work addresses functional studies of human and primate promoters, and the genome-wide localization and validation of human-specific transcription factor binding sites of the essential transcription factor GABPa. In this context, the development of an improved PCR protocol, including the careful adjustment of PCR additives to compose an efficient enhancer mix, was central to the amplification of large GC-rich promoter fragments used as source for the functional studies. Based...

  2. BDflex: A method for efficient treatment of molecular flexibility in calculating protein-ligand binding rate constants from Brownian dynamics simulations

    Science.gov (United States)

    Greives, Nicholas; Zhou, Huan-Xiang

    2012-10-01

    A method developed by Northrup et al. [J. Chem. Phys. 80, 1517 (1984)], 10.1063/1.446900 for calculating protein-ligand binding rate constants (ka) from Brownian dynamics (BD) simulations has been widely used for rigid molecules. Application to flexible molecules is limited by the formidable computational cost to treat conformational fluctuations during the long BD simulations necessary for ka calculation. Here, we propose a new method called BDflex for ka calculation that circumvents this problem. The basic idea is to separate the whole space into an outer region and an inner region, and formulate ka as the product of kE and bar η _d, which are obtained by separately solving exterior and interior problems. kE is the diffusion-controlled rate constant for the ligand in the outer region to reach the dividing surface between the outer and inner regions; in this exterior problem conformational fluctuations can be neglected. bar η _d is the probability that the ligand, starting from the dividing surface, will react at the binding site rather than escape to infinity. The crucial step in reducing the determination of bar η _d to a problem confined to the inner region is a radiation boundary condition imposed on the dividing surface; the reactivity on this boundary is proportional to kE. By confining the ligand to the inner region and imposing the radiation boundary condition, we avoid multiple-crossing of the dividing surface before reaction at the binding site and hence dramatically cut down the total simulation time, making the treatment of conformational fluctuations affordable. BDflex is expected to have wide applications in problems where conformational fluctuations of the molecules are crucial for productive ligand binding, such as in cases where transient widening of a bottleneck allows the ligand to access the binding pocket, or the binding site is properly formed only after ligand entrance induces the closure of a lid.

  3. Synthesis and Properties of Bis-Porphyrin Molecular Tweezers: Effects of Spacer Flexibility on Binding and Supramolecular Chirogenesis

    Directory of Open Access Journals (Sweden)

    Magnus Blom

    2015-12-01

    Full Text Available Ditopic binding of various dinitrogen compounds to three bisporphyrin molecular tweezers with spacers of varying conformational rigidity, incorporating the planar enediyne (1, the helical stiff stilbene (2, or the semi-rigid glycoluril motif fused to the porphyrins (3, are compared. Binding constants Ka = 104–106 M−1 reveal subtle differences between these tweezers, that are discussed in terms of porphyrin dislocation modes. Exciton coupled circular dichroism (ECCD of complexes with chiral dinitrogen guests provides experimental evidence for the conformational properties of the tweezers. The results are further supported and rationalized by conformational analysis.

  4. Genome-scale analysis of metazoan replication origins reveals their organization in specific but flexible sites defined by conserved features

    Science.gov (United States)

    Cayrou, Christelle; Coulombe, Philippe; Vigneron, Alice; Stanojcic, Slavica; Ganier, Olivier; Peiffer, Isabelle; Rivals, Eric; Puy, Aurore; Laurent-Chabalier, Sabine; Desprat, Romain; Méchali, Marcel

    2011-01-01

    In metazoans, thousands of DNA replication origins (Oris) are activated at each cell cycle. Their genomic organization and their genetic nature remain elusive. Here, we characterized Oris by nascent strand (NS) purification and a genome-wide analysis in Drosophila and mouse cells. We show that in both species most CpG islands (CGI) contain Oris, although methylation is nearly absent in Drosophila, indicating that this epigenetic mark is not crucial for defining the activated origin. Initiation of DNA synthesis starts at the borders of CGI, resulting in a striking bimodal distribution of NS, suggestive of a dual initiation event. Oris contain a unique nucleotide skew around NS peaks, characterized by G/T and C/A overrepresentation at the 5′ and 3′ of Ori sites, respectively. Repeated GC-rich elements were detected, which are good predictors of Oris, suggesting that common sequence features are part of metazoan Oris. In the heterochromatic chromosome 4 of Drosophila, Oris correlated with HP1 binding sites. At the chromosome level, regions rich in Oris are early replicating, whereas Ori-poor regions are late replicating. The genome-wide analysis was coupled with a DNA combing analysis to unravel the organization of Oris. The results indicate that Oris are in a large excess, but their activation does not occur at random. They are organized in groups of site-specific but flexible origins that define replicons, where a single origin is activated in each replicon. This organization provides both site specificity and Ori firing flexibility in each replicon, allowing possible adaptation to environmental cues and cell fates. PMID:21750104

  5. Protein stability, flexibility and function

    DEFF Research Database (Denmark)

    Teilum, Kaare; Olsen, Johan G; Kragelund, Birthe B

    2011-01-01

    Proteins rely on flexibility to respond to environmental changes, ligand binding and chemical modifications. Potentially, a perturbation that changes the flexibility of a protein may interfere with its function. Millions of mutations have been performed on thousands of proteins in quests for a...... data presented is it clear that there are specific sites (flexibility hotspots) in proteins that are important for both binding and stability. This article is part of a Special Issue entitled: Protein Dynamics: Experimental and Computational Approaches....

  6. Flexible supercapacitors

    Institute of Scientific and Technical Information of China (English)

    Shan Shi; Chengjun Xu; Cheng Yang; Jia Li; Hongda Du; Baohua Li; Feiyu Kang

    2013-01-01

    Flexible supercapacitors show a great potential for applications in wearable,miniaturized,portable,largescale transparent and flexible consumer electronics due to their significant,inherent advantages,such as being flexible,lightweight,low cost and environmentally friendly in comparison with the current energy storage devices.In this report,recent progress on flexible supercapacitors,flexible electrodes and electrolytes is reviewed.In addition,the future challenges and opportunities are discussed.

  7. Flexibility of EF-hand motifs: structural and thermodynamic studies of Calcium Binding Protein-1 from Entamoeba histolytica with Pb2+, Ba2+, and Sr2+

    International Nuclear Information System (INIS)

    EF-hand proteins can be activated by the binding of various heavy metals other than calcium, and such complexes can disturb the calcium-signaling pathway and cause toxicity and disease causing state. So far, no comprehensive study has been done to understand different heavy metals binding to calcium signaling proteins. Energetically, Ca2+ is preferred in three sites, while in one site Ba2+ has better binding energy. The Sr2+-coordination in the EF hand motifs is similar to that of the native Ca2+ bound structure, except for the lack of water coordination. Sr2+-coordination seems to be a pre-formed in nature since all seven coordinating atoms are from the protein itself, which also correlates with entropy contributions in Sr2+ binding. These findings improve our understanding of metal association with calcium binding proteins and of metal induced conformational changes.

  8. On flexibility

    OpenAIRE

    Christoph R. Weiss; Briglauer, Wolfgang

    2000-01-01

    By building on theoretical work by Mills and Schumann (1985) and Ungern-Sternberg (1990) this paper provides evidence on the determinants of two dimensions of flexibility, the flexibility in adjusting aggregate output over time (tactical flexibility) as well as the ability to switch quickly between products (operational flexibility). Econometric analysis of a sample of 40.000 farms in Upper-Austria for the period 1980 to 1990 suggests that larger full-time farms operated by younger, better ed...

  9. Transforming growth factor beta stimulation of biglycan gene expression is potentially mediated by sp1 binding factors

    DEFF Research Database (Denmark)

    Heegaard, Anne-Marie; Xie, Zhongjian; Young, Marian Frances;

    2004-01-01

    construct was co-transfected with Sp1 and Sp3 expression vectors in Sp1-deficient Drosophila Schneider-2 cells, Sp1 induced the transcriptional activity of biglycan. Addition of Sp3 augmented the effect of Sp1 on biglycan gene expression. Induction of biglycan mRNA expression in response to TGF-beta in MG......-63 cells was abrogated by mithramycin, an inhibitor of Sp1 binding to GC-rich DNA sequences. A mutation in the Sp1 site at -216 to -208 within the -218 biglycan promoter construct substantially diminished the transcriptional up-regulation by TGF-beta(1). Taken together this data shows for the first...

  10. Molecular dynamic study of MlaC protein in Gram-negative bacteria: conformational flexibility, solvent effect and protein-phospholipid binding.

    Science.gov (United States)

    Huang, Yu-Ming M; Miao, Yinglong; Munguia, Jason; Lin, Leo; Nizet, Victor; McCammon, J Andrew

    2016-08-01

    The composition of the outer membrane in Gram-negative bacteria is asymmetric, with the lipopolysaccharides found in the outer leaflet and phospholipids in the inner leaflet. The MlaC protein transfers phospholipids from the outer to inner membrane to maintain such lipid asymmetry in the Mla pathway. In this work, we have performed molecular dynamics simulations on apo and phospholipid-bound systems to study the dynamical properties of MlaC. Our simulations show that the phospholipid forms hydrophobic interactions with the protein. Residues surrounding the entrance of the binding site exhibit correlated motions to control the site opening and closing. Lipid binding leads to increase of the binding pocket volume and precludes entry of the water molecules. However, in the absence of the phospholipid, water molecules can freely move in and out of the binding site when the pocket is open. Dehydration occurs when the pocket closes. This study provides dynamic information of the MlaC protein and may facilitate the design of antibiotics against the Mla pathway of Gram-negative bacteria. PMID:27111825

  11. Hydrogen/Deuterium Exchange Mass Spectrometry Reveals Specific Changes in the Local Flexibility of Plasminogen Activator Inhibitor 1 upon Binding to the Somatomedin B Domain of Vitronectin

    DEFF Research Database (Denmark)

    Trelle, Morten Beck; Hirschberg, Daniel; Jansson, Anna; Ploug, Michael; Roepstorff, Peter; Andreasen, Peter; Jørgensen, Thomas

    2012-01-01

    The native fold of plasminogen activator inhibitor 1 (PAI-1) represents an active metastable conformation that spontaneously converts to an inactive latent form. Binding of the somatomedin B domain (SMB) of the endogenous cofactor vitronectin to PAI-1 delays the transition to the latent state and...

  12. Ligand Binding Reduces Conformational Flexibility in the Active Site of Tyrosine Phosphatase Related to Biofilm Formation A (TpbA) from Pseudomonas aeruginosa

    OpenAIRE

    Koveal, Dorothy; Clarkson, Michael W.; Wood, Thomas K.; Page, Rebecca; Peti, Wolfgang

    2013-01-01

    TpbA is a periplasmic dual specificity phosphatase (DUSP) that controls biofilm formation in the pathogenic bacterium, Pseudomonas aeruginosa. While DUSPs are known to regulate important cellular functions in both prokaryotes and eukaryotes, very few structures of bacterial DUSPs are available. Here, we present the solution structure of TpbA in the ligand-free open conformation, along with an analysis of the structural and dynamic changes that accompany ligand/phosphate binding. While TpbA ad...

  13. : Protein flexibility

    OpenAIRE

    Bornot, Aurélie; Offmann, Bernard; De Brevern, Alexandre

    2007-01-01

    Protein structures and protein structural models are great tools to reach protein function and provide very relevant information for drug design. Nevertheless, protein structures are not rigid entities. Cutting-edge bioinformatics methods tend to take into account the flexibility of these macromolecules. We present new approaches used to define protein structure flexibility.

  14. An encodable lanthanide binding tag with reduced size and flexibility for measuring residual dipolar couplings and pseudocontact shifts in large proteins.

    Science.gov (United States)

    Barb, Adam W; Subedi, Ganesh P

    2016-01-01

    Metal ions serve important roles in structural biology applications from long-range perturbations seen in magnetic resonance experiments to electron-dense signatures in X-ray crystallography data; however, the metal ion must be secured in a molecular framework to achieve the maximum benefit. Polypeptide-based lanthanide-binding tags (LBTs) represent one option that can be directly encoded within a recombinant protein expression construct. However, LBTs often exhibit significant mobility relative to the target molecule. Here we report the characterization of improved LBTs sequences for insertion into a protein loop. These LBTs were inserted to connect two parallel alpha helices of an immunoglobulin G (IgG)-binding Z domain platform. Variants A and B bound Tb(3+) with high affinity (0.70 and 0.13 μM, respectively) and displayed restricted LBT motion. Compared to the parent construct, the metal-bound A experienced a 2.5-fold reduction in tag motion as measured by magnetic field-induced residual dipolar couplings and was further studied in a 72.2 kDa complex with the human IgG1 fragment crystallizable (IgG1 Fc) glycoprotein. The appearance of both pseudo-contact shifts (-0.221 to 0.081 ppm) and residual dipolar couplings (-7.6 to 14.3 Hz) of IgG1 Fc resonances in the IgG1 Fc:(variant A:Tb(3+))2 complex indicated structural restriction of the LBT with respect to the Fc. These studies highlight the applicability of improved LBT sequences with reduced mobility to probe the structure of macromolecular systems. PMID:26728077

  15. An encodable lanthanide binding tag with reduced size and flexibility for measuring residual dipolar couplings and pseudocontact shifts in large proteins

    International Nuclear Information System (INIS)

    Metal ions serve important roles in structural biology applications from long-range perturbations seen in magnetic resonance experiments to electron-dense signatures in X-ray crystallography data; however, the metal ion must be secured in a molecular framework to achieve the maximum benefit. Polypeptide-based lanthanide-binding tags (LBTs) represent one option that can be directly encoded within a recombinant protein expression construct. However, LBTs often exhibit significant mobility relative to the target molecule. Here we report the characterization of improved LBTs sequences for insertion into a protein loop. These LBTs were inserted to connect two parallel alpha helices of an immunoglobulin G (IgG)-binding Z domain platform. Variants A and B bound Tb3+ with high affinity (0.70 and 0.13 μM, respectively) and displayed restricted LBT motion. Compared to the parent construct, the metal-bound A experienced a 2.5-fold reduction in tag motion as measured by magnetic field-induced residual dipolar couplings and was further studied in a 72.2 kDa complex with the human IgG1 fragment crystallizable (IgG1 Fc) glycoprotein. The appearance of both pseudo-contact shifts (−0.221 to 0.081 ppm) and residual dipolar couplings (−7.6 to 14.3 Hz) of IgG1 Fc resonances in the IgG1 Fc:(variant A:Tb3+)2 complex indicated structural restriction of the LBT with respect to the Fc. These studies highlight the applicability of improved LBT sequences with reduced mobility to probe the structure of macromolecular systems

  16. An encodable lanthanide binding tag with reduced size and flexibility for measuring residual dipolar couplings and pseudocontact shifts in large proteins

    Energy Technology Data Exchange (ETDEWEB)

    Barb, Adam W., E-mail: abarb@iastate.edu; Subedi, Ganesh P. [Iowa State University, Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology (United States)

    2016-01-15

    Metal ions serve important roles in structural biology applications from long-range perturbations seen in magnetic resonance experiments to electron-dense signatures in X-ray crystallography data; however, the metal ion must be secured in a molecular framework to achieve the maximum benefit. Polypeptide-based lanthanide-binding tags (LBTs) represent one option that can be directly encoded within a recombinant protein expression construct. However, LBTs often exhibit significant mobility relative to the target molecule. Here we report the characterization of improved LBTs sequences for insertion into a protein loop. These LBTs were inserted to connect two parallel alpha helices of an immunoglobulin G (IgG)-binding Z domain platform. Variants A and B bound Tb{sup 3+} with high affinity (0.70 and 0.13 μM, respectively) and displayed restricted LBT motion. Compared to the parent construct, the metal-bound A experienced a 2.5-fold reduction in tag motion as measured by magnetic field-induced residual dipolar couplings and was further studied in a 72.2 kDa complex with the human IgG1 fragment crystallizable (IgG1 Fc) glycoprotein. The appearance of both pseudo-contact shifts (−0.221 to 0.081 ppm) and residual dipolar couplings (−7.6 to 14.3 Hz) of IgG1 Fc resonances in the IgG1 Fc:(variant A:Tb{sup 3+}){sub 2} complex indicated structural restriction of the LBT with respect to the Fc. These studies highlight the applicability of improved LBT sequences with reduced mobility to probe the structure of macromolecular systems.

  17. Binding water to a PEG-linked flexible bichromophore: IR spectra of diphenoxyethane-(H2O)n clusters, n = 2-4

    International Nuclear Information System (INIS)

    The single-conformation infrared (IR) and ultraviolet (UV) spectroscopies of neutral 1,2-diphenoxyethane-(H2O)n clusters with n = 2-4 (labeled henceforth as 1:n) have been studied in a molecular beam using a combination of resonant two-photon ionization, IR-UV holeburning, and resonant ion-dip infrared (RIDIR) spectroscopies. Ground state RIDIR spectra in the OH and CH stretch regions were used to provide firm assignments for the structures of the clusters by comparing the experimental spectra with the predictions of calculations carried out at the density functional M05-2X/6-31+G(d) level of theory. At all sizes in this range, the water molecules form water clusters in which all water molecules engage in a single H-bonded network. Selective binding to the tgt monomer conformer of 1,2-diphenoxyethane (C6H5-O-CH2-CH2-O-C6H5, DPOE) occurs, since this conformer provides a binding pocket in which the two ether oxygens and two phenyl ring π clouds can be involved in stabilizing the water cluster. The 1:2 cluster incorporates a water dimer “chain” bound to DPOE much as it is in the 1:1 complex [E. G. Buchanan et al., J. Phys. Chem. Lett. 4, 1644 (2013)], with primary attachment via a double-donor water that bridges the ether oxygen of one phenoxy group and the π cloud of the other. Two conformers of the 1:3 cluster are observed and characterized, one that extends the water chain to a third molecule (1:3 chain) and the other incorporating a water trimer cycle (1:3 cycle). A cyclic water structure is also observed for the 1:4 cluster. These structural characterizations provide a necessary foundation for studies of the perturbations imposed on the two close-lying S1/S2 excited states of DPOE considered in the adjoining paper [P. S. Walsh et al., J. Chem. Phys. 142, 154304 (2015)

  18. Binding water to a PEG-linked flexible bichromophore: IR spectra of diphenoxyethane-(H2O)n clusters, n = 2-4

    Science.gov (United States)

    Walsh, Patrick S.; Buchanan, Evan G.; Gord, Joseph R.; Zwier, Timothy S.

    2015-04-01

    The single-conformation infrared (IR) and ultraviolet (UV) spectroscopies of neutral 1,2-diphenoxyethane-(H2O)n clusters with n = 2-4 (labeled henceforth as 1:n) have been studied in a molecular beam using a combination of resonant two-photon ionization, IR-UV holeburning, and resonant ion-dip infrared (RIDIR) spectroscopies. Ground state RIDIR spectra in the OH and CH stretch regions were used to provide firm assignments for the structures of the clusters by comparing the experimental spectra with the predictions of calculations carried out at the density functional M05-2X/6-31+G(d) level of theory. At all sizes in this range, the water molecules form water clusters in which all water molecules engage in a single H-bonded network. Selective binding to the tgt monomer conformer of 1,2-diphenoxyethane (C6H5-O-CH2-CH2-O-C6H5, DPOE) occurs, since this conformer provides a binding pocket in which the two ether oxygens and two phenyl ring π clouds can be involved in stabilizing the water cluster. The 1:2 cluster incorporates a water dimer "chain" bound to DPOE much as it is in the 1:1 complex [E. G. Buchanan et al., J. Phys. Chem. Lett. 4, 1644 (2013)], with primary attachment via a double-donor water that bridges the ether oxygen of one phenoxy group and the π cloud of the other. Two conformers of the 1:3 cluster are observed and characterized, one that extends the water chain to a third molecule (1:3 chain) and the other incorporating a water trimer cycle (1:3 cycle). A cyclic water structure is also observed for the 1:4 cluster. These structural characterizations provide a necessary foundation for studies of the perturbations imposed on the two close-lying S1/S2 excited states of DPOE considered in the adjoining paper [P. S. Walsh et al., J. Chem. Phys. 142, 154304 (2015)].

  19. Proto-oncogene FBI-1 (Pokemon/ZBTB7A) Represses Transcription of the Tumor Suppressor Rb Gene via Binding Competition with Sp1 and Recruitment of Co-repressors*S⃞

    OpenAIRE

    Jeon, Bu-Nam; Yoo, Jung-Yoon; Choi, Won-Il; Lee, Choong-Eun; Yoon, Ho-Geun; Hur, Man-Wook

    2008-01-01

    FBI-1 (also called Pokemon/ZBTB7A) is a BTB/POZ-domain Krüppel-like zinc-finger transcription factor. Recently, FBI-1 was characterized as a proto-oncogenic protein, which represses tumor suppressor ARF gene transcription. The expression of FBI-1 is increased in many cancer tissues. We found that FBI-1 potently represses transcription of the Rb gene, a tumor suppressor gene important in cell cycle arrest. FBI-1 binds to four GC-rich promoter elements (FREs) located at ...

  20. Flexible Capitalism

    DEFF Research Database (Denmark)

    Approaching “work” as at heart a practice of exchange, this volume explores sociality in work environments marked by the kind of structural changes that have come to define contemporary “flexible” capitalism. It introduces anthropological exchange theory to a wider readership, and shows how the...... perspective offers new ways to enquire about the flexible capitalism’s social dimensions. The essays contribute to a trans-disciplinary scholarship on contemporary economic practice and change by documenting how, across diverse settings, “gift-like” socialities proliferate, and even sustain the intensified...... flexible commoditization that more commonly is touted as tearing social relations apart. By interrogating a keenly debated contemporary work regime through an approach to sociality rooted in a rich and distinct anthropological legacy, the volume also makes a novel contribution to the anthropological...

  1. Development of Flexible Bearing

    Directory of Open Access Journals (Sweden)

    K.S.Mohanraj

    2014-06-01

    Full Text Available Elastomeric base isolation systems are proven to be effective in reducing seismic forces transmitted to buildings. However, due to their cost, the use of these devices is currently limited to large and expensive buildings. A fiber reinforced elastomeric isolator utilizes fiber fabric, such as carbon fiber, glass fibre, and etc. as the reinforcement material instead of solid steel plates. The fibre fabric reinforcement is extensible in tension and has no flexural rigidity. Elastomers normally used in the isolator are natural rubber; neoprene, butyl rubber and nit rile rubber etc. These devices were fabricated by binding alternating layers of rubber and fibre mesh. The fibre mesh is used to increase the vertical stiffness of the bearings while maintaining low lateral stiffness. Characterizing the behaviour of a fibre reinforced bearing “shape factor” of the bearing, Poisson’s ratio of the elastomeric material and flexibility of the reinforcing sheets and investigate the effect of reinforcement flexibility on compressive behaviour of elastomeric bearings with different geometrical and material properties. Bonding with fibre reinforcements can increase the stiffness of elastic layers only when the elastic layer is compressed.

  2. FLEXIBILITY IN AGRICULTURE

    OpenAIRE

    Christoph R. Weiss

    1999-01-01

    This paper considers the determinants of two dimensions of flexibility, the flexibility in adjusting aggregate output over time ("tactical flexibility") as well as the ability to switch quickly between products ("operational flexibility"). Econometric analysis of a sample of 40,000 farms in Upper-Austria for the period 1980 to 1990 suggests that larger full-time farms operated by younger, better educated farm operators are more flexible, ceteris paribus. The results further indicate a signifi...

  3. Flexible distribution network - Local flexibility and aggregation

    OpenAIRE

    Høivik, Øivind

    2015-01-01

    The increasing intermittent production from wind power and solar-PV creates a system that is more complex to plan, control and balance. At the same time, there is an increase in power consumption due to electric vehicles, heat pumps and other energy-intensive appliances. The volatile system indicates a need for flexibility. The flexibility could come from distributed generation (DG), local storage or flexible demand. However, the contribution from a single resource may be too small to make an...

  4. BOOK REVIEW FLEXIBLE PEDAGOGY AND FLEXIBLE PRACTICE

    OpenAIRE

    Hakan KILINC

    2016-01-01

    The term of “flexible learning” means that the learner’s any time and any place involvement of the learning process without limitations on time and location. It can be said that flexible learning opportunities are provided for the learners via digital environments as open and distance learning environments. By this means, the learners can reach the learning contents without having to be in a particular location. Therefore, it can be seen that learning environments having flexible structures a...

  5. Flexible Memory Networks

    OpenAIRE

    Curto, Carina; Degeratu, Anda; Itskov, Vladimir

    2010-01-01

    Networks of neurons in some brain areas are flexible enough to encode new memories quickly. Using a standard firing rate model of recurrent networks, we develop a theory of flexible memory networks. Our main results characterize networks having the maximal number of flexible memory patterns, given a constraint graph on the network's connectivity matrix. Modulo a mild topological condition, we find a close connection between maximally flexible networks and rank 1 matrices. The topological cond...

  6. Highly flexible protein-peptide docking using CABS-dock

    OpenAIRE

    Ciemny, Maciej Pawel; Kurcinski, Mateusz; Kozak, Konrad Jakub; Kolinski, Andrzej; Kmiecik, Sebastian

    2016-01-01

    Protein-peptide molecular docking is a difficult modeling problem. It is even more challenging when significant conformational changes that may occur during the binding process need to be predicted. In this chapter, we demonstrate the capabilities and features of the CABS-dock server for flexible protein-peptide docking. CABS-dock allows highly efficient modeling of full peptide flexibility and significant flexibility of a protein receptor. During CABS-dock docking, the peptide folding and bi...

  7. Specificity and affinity quantification of flexible recognition from underlying energy landscape topography.

    Directory of Open Access Journals (Sweden)

    Xiakun Chu

    2014-08-01

    Full Text Available Flexibility in biomolecular recognition is essential and critical for many cellular activities. Flexible recognition often leads to moderate affinity but high specificity, in contradiction with the conventional wisdom that high affinity and high specificity are coupled. Furthermore, quantitative understanding of the role of flexibility in biomolecular recognition is still challenging. Here, we meet the challenge by quantifying the intrinsic biomolecular recognition energy landscapes with and without flexibility through the underlying density of states. We quantified the thermodynamic intrinsic specificity by the topography of the intrinsic binding energy landscape and the kinetic specificity by association rate. We found that the thermodynamic and kinetic specificity are strongly correlated. Furthermore, we found that flexibility decreases binding affinity on one hand, but increases binding specificity on the other hand, and the decreasing or increasing proportion of affinity and specificity are strongly correlated with the degree of flexibility. This shows more (less flexibility leads to weaker (stronger coupling between affinity and specificity. Our work provides a theoretical foundation and quantitative explanation of the previous qualitative studies on the relationship among flexibility, affinity and specificity. In addition, we found that the folding energy landscapes are more funneled with binding, indicating that binding helps folding during the recognition. Finally, we demonstrated that the whole binding-folding energy landscapes can be integrated by the rigid binding and isolated folding energy landscapes under weak flexibility. Our results provide a novel way to quantify the affinity and specificity in flexible biomolecular recognition.

  8. Molecular insights into DNA binding and anchoring by the Bacillus subtilis sporulation kinetochore-like RacA protein

    Science.gov (United States)

    Schumacher, Maria A.; Lee, Jeehyun; Zeng, Wenjie

    2016-01-01

    During Bacillus subtilis sporulation, segregating sister chromosomes are anchored to cell poles and the chromosome is remodeled into an elongated structure called the axial filament. Data indicate that a developmentally regulated protein called RacA is involved in these functions. To gain insight into how RacA performs these diverse processes we performed a battery of structural and biochemical analyses. These studies show that RacA contains an N-terminal winged-helix-turn-helix module connected by a disordered region to a predicted coiled-coil domain. Structures capture RacA binding the DNA using distinct protein–protein interfaces and employing adjustable DNA docking modes. This unique DNA binding mechanism indicates how RacA can both specifically recognize its GC-rich centromere and also non-specifically bind the DNA. Adjacent RacA molecules within the protein–DNA structure interact leading to DNA compaction, suggesting a mechanism for axial filament formation. We also show that the RacA C-domain coiled coil directly contacts the coiled coil region of the polar protein DivIVA, which anchors RacA and hence the chromosome to the pole. Thus, our combined data reveal unique DNA binding properties by RacA and provide insight into the DNA remodeling and polar anchorage functions of the protein. PMID:27085804

  9. Global Sourcing Flexibility

    DEFF Research Database (Denmark)

    Ørberg Jensen, Peter D.; Petersen, Bent

    2013-01-01

    Recent studies show that flexibility is a key concern for firms that engage in the global sourcing of services. In this conceptual paper, we seek to explore two central aspects of global sourcing flexibility: In the first part of the paper, we provide a definition of the construct of global...... sourcing flexibility. Here we draw on prior research in the fields of organizational flexibility, international business and global sourcing as well as case examples and secondary studies. In the second part of the paper, we discuss the implications of global sourcing flexibility for firm strategy and...... operations against the backdrop of the theory-based definition of the construct. We discuss in particular the importance of global sourcing flexibility for operational performance stability, and the trade-off between specialization benefits, emerging from location and service provider specialization, versus...

  10. MD and NMR analyses of choline and TMA binding to duplex DNA: on the origins of aberrant sequence-dependent stability by alkyl cations in aqueous and water-free solvents.

    Science.gov (United States)

    Portella, Guillem; Germann, Markus W; Hud, Nicholas V; Orozco, Modesto

    2014-02-26

    It has been known for decades that alkylammonium ions, such as tetramethyl ammonium (TMA), alter the usual correlation between DNA GC-content and duplex stability. In some cases it is even possible for an AT-rich duplex to be more stable than a GC-rich duplex of the same length. There has been much speculation regarding the origin of this aberration in sequence-dependent DNA duplex stability, but no clear resolution. Using a combination of molecular dynamics simulations and NMR spectroscopy we demonstrate that choline (2-hydroxy-N,N,N-trimethylethanaminium) and TMA are preferentially localized in the minor groove of DNA duplexes at A·T base pairs and these same ions show less pronounced localization in the major groove compared to what has been demonstrated for alkali and alkali earth metal ions. Furthermore, free energy calculations show that single-stranded GC-rich sequences exhibit more favorable solvation by choline than single-stranded AT-rich sequences. The sequence-specific nature of choline and TMA binding provides a rationale for the enhanced stability of AT-rich sequences when alkyl-ammonium ions are used as the counterions of DNA. Our combined theoretical and experimental study provides one of the most detailed pictures to date of cations localized along DNA in the solution state, and provides insights that go beyond understanding alkyl-ammonium ion binding to DNA. In particular, because choline and TMA bind to DNA in a manner that is found to be distinct from that previously reported for Na(+), K(+), Mg(2+), and Ca(2+), our results reveal the important but underappreciated role that most other cations play in sequence-specific duplex stability. PMID:24490755

  11. Reversibly Bistable Flexible Electronics

    KAUST Repository

    Alfaraj, Nasir

    2015-05-01

    Introducing the notion of transformational silicon electronics has paved the way for integrating various applications with silicon-based, modern, high-performance electronic circuits that are mechanically flexible and optically semitransparent. While maintaining large-scale production and prototyping rapidity, this flexible and translucent scheme demonstrates the potential to transform conventionally stiff electronic devices into thin and foldable ones without compromising long-term performance and reliability. In this work, we report on the fabrication and characterization of reversibly bistable flexible electronic switches that utilize flexible n-channel metal-oxide-semiconductor field-effect transistors. The transistors are fabricated initially on rigid (100) silicon substrates before they are peeled off. They can be used to control flexible batches of light-emitting diodes, demonstrating both the relative ease of scaling at minimum cost and maximum reliability and the feasibility of integration. The peeled-off silicon fabric is about 25 µm thick. The fabricated devices are transferred to a reversibly bistable flexible platform through which, for example, a flexible smartphone can be wrapped around a user’s wrist and can also be set back to its original mechanical position. Buckling and cyclic bending of such host platforms brings a completely new dimension to the development of flexible electronics, especially rollable displays.

  12. Flexible magnetoimpidence sensor

    KAUST Repository

    Kavaldzhiev, M. N.

    2015-05-01

    Recently, flexible electronic devices have attracted increasing interest, due to the opportunities they promise for new applications such as wearable devices, where the components are required to flex during normal use[1]. In this light, different magnetic sensors, like microcoil, spin valve, giant magnetoresistance (GMR), magnetoimpedance (MI), have been studied previously on flexible substrates.

  13. Shield For Flexible Pipe

    Science.gov (United States)

    Ponton, Michael K.; Williford, Clifford B.; Lagen, Nicholas T.

    1995-01-01

    Cylindrical shield designed to fit around flexible pipe to protect nearby workers from injury and equipment from damage if pipe ruptures. Designed as pressure-relief device. Absorbs impact of debris ejected radially from broken flexible pipe. Also redirects flow of pressurized fluid escaping from broken pipe onto flow path allowing for relief of pressure while minimizing potential for harm.

  14. Binding of the biogenic polyamines to deoxyribonucleic acids of varying base composition: base specificity and the associated energetics of the interaction.

    Directory of Open Access Journals (Sweden)

    Ayesha Kabir

    Full Text Available BACKGROUND: The thermodynamics of the base pair specificity of the binding of the polyamines spermine, spermidine, putrescine, and cadaverine with three genomic DNAs Clostridium perfringens, 27% GC, Escherichia coli, 50% GC and Micrococcus lysodeikticus, 72% GC have been studied using titration calorimetry and the data supplemented with melting studies, ethidium displacement and circular dichroism spectroscopy results. METHODOLOGY/PRINCIPAL FINDINGS: Isothermal titration calorimetry, differential scanning calorimetry, optical melting studies, ethidium displacement, circular dichroism spectroscopy are the various techniques employed to characterize the interaction of four polyamines, spermine, spermidine, putersine and cadaverine with the DNAs. Polyamines bound stronger with AT rich DNA compared to the GC rich DNA and the binding varied depending on the charge on the polyamine as spermine>spermidine >putrescine>cadaverine. Thermodynamics of the interaction revealed that the binding was entropy driven with small enthalpy contribution. The binding was influenced by salt concentration suggesting the contribution from electrostatic forces to the Gibbs energy of binding to be the dominant contributor. Each system studied exhibited enthalpy-entropy compensation. The negative heat capacity changes suggested a role for hydrophobic interactions which may arise due to the non polar interactions between DNA and polyamines. CONCLUSION/SIGNIFICANCE: From a thermodynamic analysis, the AT base specificity of polyamines to DNAs has been elucidated for the first time and supplemented by structural studies.

  15. Rolling cuff flexible bellows

    Science.gov (United States)

    Lambert, Donald R.

    1985-01-01

    A flexible connector apparatus used to join two stiff non-deformable members, such as piping. The apparatus is provided with one or more flexible sections or assemblies each utilizing a bellows of a rolling cuff type connected between two ridge members, with the bellows being supported by a back-up ring, such that only the curved end sections of the bellows are unsupported. Thus, the bellows can be considered as being of a tube-shaped configuration and thus have high pressure resistance. The components of the flexible apparatus are sealed or welded one to another such that it is fluid tight.

  16. Wage Flexibility in Contemporary Society

    OpenAIRE

    Silviu Alexandru TECULESCU

    2008-01-01

    The present article has as an objective to offer the most efficient solutions so that our country reach the level of economic development associated to the West European states. It proposes the division of flexibility in three components, namely: internal flexibility, external flexibility and wage flexibility. The analysis performed within the present study will emphasize the wage flexibility. Wage flexibility can be classified in four components: ¦S Plans of individual and group incentives; ...

  17. Flexible Word Classes

    DEFF Research Database (Denmark)

    van Lier, Eva; Rijkhoff, Jan

    2013-01-01

    • First major publication on the phenomenon • Offers cross-linguistic, descriptive, and diverse theoretical approaches • Includes analysis of data from different language families and from lesser studied languages This book is the first major cross-linguistic study of 'flexible words', i.e. words...... that cannot be classified in terms of the traditional lexical categories Verb, Noun, Adjective or Adverb. Flexible words can - without special morphosyntactic marking - serve in functions for which other languages must employ members of two or more of the four traditional, 'specialised' word classes....... Thus, flexible words are underspecified for communicative functions like 'predicating' (verbal function), 'referring' (nominal function) or 'modifying' (a function typically associated with adjectives and e.g. manner adverbs). Even though linguists have been aware of flexible world classes for more...

  18. Flexibility in insulin prescription

    Science.gov (United States)

    Kalra, Sanjay; Gupta, Yashdeep; Unnikrishnan, Ambika Gopalakrishnan

    2016-01-01

    This communication explores the concept of flexibility, a propos insulin preparations and insulin regimes used in the management of type 2 diabetes. The flexibility of an insulin regime or preparation is defined as their ability to be injected at variable times, with variable injection-meal time gaps, in a dose frequency and quantum determined by shared decision making, with a minimal requirement of glucose monitoring and health professional consultation, with no compromise on safety, efficiency and tolerability. The relative flexibility of various basal, prandial and dual action insulins, as well as intensive regimes, is compared. The biopsychosocial model of health is used to assess the utility of different insulins while encouraging a philosophy of flexible insulin usage. PMID:27186563

  19. Flexibility in insulin prescription.

    Science.gov (United States)

    Kalra, Sanjay; Gupta, Yashdeep; Unnikrishnan, Ambika Gopalakrishnan

    2016-01-01

    This communication explores the concept of flexibility, a propos insulin preparations and insulin regimes used in the management of type 2 diabetes. The flexibility of an insulin regime or preparation is defined as their ability to be injected at variable times, with variable injection-meal time gaps, in a dose frequency and quantum determined by shared decision making, with a minimal requirement of glucose monitoring and health professional consultation, with no compromise on safety, efficiency and tolerability. The relative flexibility of various basal, prandial and dual action insulins, as well as intensive regimes, is compared. The biopsychosocial model of health is used to assess the utility of different insulins while encouraging a philosophy of flexible insulin usage. PMID:27186563

  20. Flexibility in insulin prescription

    Directory of Open Access Journals (Sweden)

    Sanjay Kalra

    2016-01-01

    Full Text Available This communication explores the concept of flexibility, a propos insulin preparations and insulin regimes used in the management of type 2 diabetes. The flexibility of an insulin regime or preparation is defined as their ability to be injected at variable times, with variable injection-meal time gaps, in a dose frequency and quantum determined by shared decision making, with a minimal requirement of glucose monitoring and health professional consultation, with no compromise on safety, efficiency and tolerability. The relative flexibility of various basal, prandial and dual action insulins, as well as intensive regimes, is compared. The biopsychosocial model of health is used to assess the utility of different insulins while encouraging a philosophy of flexible insulin usage.

  1. Flexible memory networks.

    Science.gov (United States)

    Curto, Carina; Degeratu, Anda; Itskov, Vladimir

    2012-03-01

    Networks of neurons in some brain areas are flexible enough to encode new memories quickly. Using a standard firing rate model of recurrent networks, we develop a theory of flexible memory networks. Our main results characterize networks having the maximal number of flexible memory patterns, given a constraint graph on the network's connectivity matrix. Modulo a mild topological condition, we find a close connection between maximally flexible networks and rank 1 matrices. The topological condition is H (1)(X;ℤ)=0, where X is the clique complex associated to the network's constraint graph; this condition is generically satisfied for large random networks that are not overly sparse. In order to prove our main results, we develop some matrix-theoretic tools and present them in a self-contained section independent of the neuroscience context. PMID:21826564

  2. Flexible Audio Coder

    OpenAIRE

    Asteborg, Marcus

    2011-01-01

    As modern communications networks are heterogeneous and, therefore, highly variable, the design of source coders should reflect this network variability. It is desired that source coders are able to adapt instantly to any bit-rate constraint. Source coders that possess this property offer coding flexibility that facilitates optimal utilization of the available communication channel within a heterogeneous network. Flexible coders are able to utilize feedback information and therefore perform a...

  3. Flexible displays, rigid designs?

    DEFF Research Database (Denmark)

    Hornbæk, Kasper

    2015-01-01

    Rapid technological progress has enabled a wide range of flexible displays for computing devices, but the user experience--which we're only beginning to understand--will be the key driver for successful designs.......Rapid technological progress has enabled a wide range of flexible displays for computing devices, but the user experience--which we're only beginning to understand--will be the key driver for successful designs....

  4. Binding Procurement

    Science.gov (United States)

    Rao, Gopalakrishna M.; Vaidyanathan, Hari

    2007-01-01

    This viewgraph presentation reviews the use of the binding procurement process in purchasing Aerospace Flight Battery Systems. NASA Engineering and Safety Center (NESC) requested NASA Aerospace Flight Battery Systems Working Group to develop a set of guideline requirements document for Binding Procurement Contracts.

  5. Flexible sucker rod unit

    Energy Technology Data Exchange (ETDEWEB)

    Allen, L.F.

    1987-02-03

    This patent describes a deep well having: a. an education tube with an inside diameter extending from the surface of the earth to far below the surface, b. a reciprocating pump housing attached to the bottom of the education tube, c. pump jack means at the surface for reciprocating the pump, d. a light sucker rod connected to the pump jack means and extending into the education tube, and e. a series of heavy sinker bars having a large cross sectional area in the education tube connecting the light sucker rod to the pump; f. an improved integral metal flexible rod unit interconnecting the sinker bars comprising in combination with the above: g. a coupling on each end of the integral metal flexible rod unit connecting the flexible rod unit to the contiguous sinker bar, h. a segment which is flexible as compared to the sinker bars connecting one of the couplings to i. an integral metal bearing adjacent to the other of the couplings, the bearing having j. a cylindrical surface with k. a diameter i. only slightly smaller than the inside diameter of the education tube thereby forming a sliding fit therewith, and ii. greater than the diameter of any other portion of the flexible rod unit and the sinker bar, and l. grooves in the cylindrical surface for the passage of fluid between in the education tube around the bearing.

  6. Flexible transparent electrode

    Science.gov (United States)

    Demiryont, Hulya; Shannon, Kenneth C., III; Moorehead, David; Bratcher, Matthew

    2011-06-01

    This paper presents the properties of the EclipseTECTM transparent conductor. EclipseTECTM is a room temperature deposited nanostructured thin film coating system comprised of metal-oxide semiconductor elements. The system possesses metal-like conductivity and glass-like transparency in the visible region. These highly conductive TEC films exhibit high shielding efficiency (35dB at 1 to 100GHz). EclipseTECTM can be deposited on rigid or flexible substrates. For example, EclipseTECTM deposited on polyethylene terephthalate (PET) is extremely flexible that can be rolled around a 9mm diameter cylinder with little or no reduction in electrical conductivity and that can assume pre-extension states after an applied stress is relieved. The TEC is colorless and has been tailored to have high visible transmittance which matches the eye sensitivity curve and allows the viewing of true background colors through the coating. EclipseTECTM is flexible, durable and can be tailored at the interface for applications such as electron- or hole-injecting OLED electrodes as well as electrodes in flexible displays. Tunable work function and optical design flexibility also make EclipseTECTM well-suited as a candidate for grid electrode replacement in next-generation photovoltaic cells.

  7. Relevance of DNA binding to the mechanism of anti-herpesvirus activity of benzhydrazone.

    Science.gov (United States)

    Palu, G; Tognon, M; Romanelli, M G; Rassu, M; Parolin, M C; Zagotto, G; Palumbo, M

    1993-04-01

    Benzhydrazone (1H-benz(f)indene-1,3(2H)-dione bis (amidino-hydrazone) (BH) is a synthetic compound with selective anti-herpesvirus activity. Its selectivity seems to stem from the inhibition of viral protein glycosylation and several hypotheses have been formulated to explain such an effect. Data presented here demonstrate that DNA binding is a prominent feature of BH. Interaction is taking place with a relatively high affinity constant and is more efficient for GC-rich viral sequences. Experiments with the cloned DNA fragments from a BH-resistant virus strain indicate that BH-DNA complex formation is drastically reduced as compared to BH-sensitive virus. The occurrence of the resistant phenotype in HEp-2 cells but not in Vero cells could be explained by differences in BH cytotoxicity. Changes in drug uptake and accumulation by cells following infection, in addition to GC preference, may also account for the degree of antiviral selectivity shown by BH. PMID:8387259

  8. Secondary Structure Preferences of Mn2+ Binding Sites in Bacterial Proteins

    Directory of Open Access Journals (Sweden)

    Tatyana Aleksandrovna Khrustaleva

    2014-01-01

    Full Text Available 3D structures of proteins with coordinated Mn2+ ions from bacteria with low, average, and high genomic GC-content have been analyzed (149 PDB files were used. Major Mn2+ binders are aspartic acid (6.82% of Asp residues, histidine (14.76% of His residues, and glutamic acid (3.51% of Glu residues. We found out that the motif of secondary structure “beta strand-major binder-random coil” is overrepresented around all the three major Mn2+ binders. That motif may be followed by either alpha helix or beta strand. Beta strands near Mn2+ binding residues should be stable because they are enriched by such beta formers as valine and isoleucine, as well as by specific combinations of hydrophobic and hydrophilic amino acid residues characteristic to beta sheet. In the group of proteins from GC-rich bacteria glutamic acid residues situated in alpha helices frequently coordinate Mn2+ ions, probably, because of the decrease of Lys usage under the influence of mutational GC-pressure. On the other hand, the percentage of Mn2+ sites with at least one amino acid in the “beta strand-major binder-random coil” motif of secondary structure (77.88% does not depend on genomic GC-content.

  9. Service-based flexible workflow system for virtual enterprise

    Institute of Scientific and Technical Information of China (English)

    WU Shao-fei

    2008-01-01

    Using the services provided by virtual enterprises, we presented a solution to implement flexible inter-enterprise workflow management. Services were the responses of events that can be accessed programmatically on the Internet by HTTP protocol. Services were obtained according to some standardized service templates. The workflow engine's flexible control to a request was bound to appropriate services and their providers by using a constraint-based, dynamic binding mechanism. Hence, a flexible and collaborative business was achieved. The workflow management system supports virtual enterprise, and the styles of virtual enterprises can be adjusted readily to adapt various situations.

  10. Pneumatic flexible shaft couplings

    Directory of Open Access Journals (Sweden)

    Jaroslav HOMIŠIN

    2007-01-01

    Full Text Available Main effort of every design engineer is reduction of torsional oscillation in any mechanical system. At present this problem can be solved by means of a suitable modification of dynamic properties of flexible shaft couplings according to dynamics in the given systems. But the dynamic properties of nowadays-applied flexible couplings arenot unchangeable because of aging and fatigue processes occurring in flexible coupling elements. Result of this fact causes detuning of mechanical system. Taking into consideration the above-mentioned situation, we suggest for mechanical systems application of a newly developed pneumatic couplings that have constant characteristicfeatures during the whole current operation and thus they have a positive influence on the system running.

  11. Flexible magnetoimpedance sensor

    KAUST Repository

    Li, Bodong

    2015-03-01

    Flexible magnetoimpedance (MI) sensors fabricated using a NiFe/Cu/NiFe tri-layer on Kapton substrate have been studied. A customized flexible microstrip transmission line was employed to investigate the MI sensors\\'s magnetic field and frequency responses and their dependence on the sensors\\'s deflection. For the first time, the impedance characteristic is obtained through reflection coefficient analysis over a wide range of frequencies from 0.1 MHz to 3 GHz and for deflections ranging from zero curvature to a radius of 7.2 cm. The sensor element maintains a high MI ratio of up to 90% and magnetic sensitivity of up to 9.2%/Oe over different bending curvatures. The relationship between the curvature and material composition is discussed based on the magnetostriction effect and stress simulations. The sensor\\'s large frequency range, simple fabrication process and high sensitivity provide a great potential for flexible electronics and wireless applications.

  12. Chaperone-assisted translocation of flexible polymers in three dimensions

    CERN Document Server

    Suhonen, P M

    2016-01-01

    Polymer translocation through a nanometer-scale pore assisted by chaperones binding to the polymer is a process encountered in vivo for proteins. Studying the relevant models by computer simulations is computationally demanding. Accordingly, previous studies are either for stiff polymers in three dimensions or flexible polymers in two dimensions. Here, we study chaperone-assisted translocation of flexible polymers in three dimensions using Langevin dynamics. We show that differences in binding mechanisms, more specifically, whether a chaperone can bind to a single or multiple sites on the polymer, lead to substantial differences in translocation dynamics in three dimensions. We show that the single-binding mode leads to dynamics that is very much like that in the constant-force driven translocation and accordingly mainly determined by tension propagation on the cis side. We obtain $\\beta \\approx 1.26$ for the exponent for the scaling of the translocation time with polymer length. This fairly low value can be ...

  13. Proto-oncogene FBI-1 (Pokemon/ZBTB7A) represses transcription of the tumor suppressor Rb gene via binding competition with Sp1 and recruitment of co-repressors.

    Science.gov (United States)

    Jeon, Bu-Nam; Yoo, Jung-Yoon; Choi, Won-Il; Lee, Choong-Eun; Yoon, Ho-Geun; Hur, Man-Wook

    2008-11-28

    FBI-1 (also called Pokemon/ZBTB7A) is a BTB/POZ-domain Krüppel-like zinc-finger transcription factor. Recently, FBI-1 was characterized as a proto-oncogenic protein, which represses tumor suppressor ARF gene transcription. The expression of FBI-1 is increased in many cancer tissues. We found that FBI-1 potently represses transcription of the Rb gene, a tumor suppressor gene important in cell cycle arrest. FBI-1 binds to four GC-rich promoter elements (FREs) located at bp -308 to -188 of the Rb promoter region. The Rb promoter also contains two Sp1 binding sites: GC-box 1 (bp -65 to -56) and GC-box 2 (bp -18 to -9), the latter of which is also bound by FBI-1. We found that FRE3 (bp -244 to -236) is also a Sp1 binding element. FBI-1 represses transcription of the Rb gene not only by binding to the FREs, but also by competing with Sp1 at the GC-box 2 and the FRE3. By binding to the FREs and/or the GC-box, FBI-1 represses transcription of the Rb gene through its POZ-domain, which recruits a co-repressor-histone deacetylase complex and deacetylates histones H3 and H4 at the Rb gene promoter. FBI-1 inhibits C2C12 myoblast cell differentiation by repressing Rb gene expression. PMID:18801742

  14. Flexible energy systems

    DEFF Research Database (Denmark)

    Lund, Henrik

    2003-01-01

    The paper discusses and analyses diffent national strategies and points out key changes in the energy system in order to achieve a system which can benefit from a high percentage of wind and CHP without having surplus production problems, introduced here as a flexible energy system....

  15. Flexible metal bellows

    CERN Multimedia

    1975-01-01

    A set of flexible metal bellows being fatigue-tested by repeated offset motion. Such bellows assemblies were used in the SPS vacuum system at places where , for instance, beam stoppers and collimators had to be moved frequently in and out of the beam path.

  16. FSD- FLEXIBLE SPACECRAFT DYNAMICS

    Science.gov (United States)

    Fedor, J. V.

    1994-01-01

    The Flexible Spacecraft Dynamics and Control program (FSD) was developed to aid in the simulation of a large class of flexible and rigid spacecraft. FSD is extremely versatile and can be used in attitude dynamics and control analysis as well as in-orbit support of deployment and control of spacecraft. FSD has been used to analyze the in-orbit attitude performance and antenna deployment of the RAE and IMP class satellites, and the HAWKEYE, SCATHA, EXOS-B, and Dynamics Explorer flight programs. FSD is applicable to inertially-oriented spinning, earth oriented, or gravity gradient stabilized spacecraft. The spacecraft flexibility is treated in a continuous manner (instead of finite element) by employing a series of shape functions for the flexible elements. Torsion, bending, and three flexible modes can be simulated for every flexible element. FSD can handle up to ten tubular elements in an arbitrary orientation. FSD is appropriate for studies involving the active control of pointed instruments, with options for digital PID (proportional, integral, derivative) error feedback controllers and control actuators such as thrusters and momentum wheels. The input to FSD is in four parts: 1) Orbit Construction FSD calculates a Keplerian orbit with environmental effects such as drag, magnetic torque, solar pressure, thermal effects, and thruster adjustments; or the user can supply a GTDS format orbit tape for a particular satellite/time-span; 2) Control words - for options such as gravity gradient effects, control torques, and integration ranges; 3) Mathematical descriptions of spacecraft, appendages, and control systems- including element geometry, properties, attitudes, libration damping, tip mass inertia, thermal expansion, magnetic tracking, and gimbal simulation options; and 4) Desired state variables to output, i.e., geometries, bending moments, fast Fourier transform plots, gimbal rotation, filter vectors, etc. All FSD input is of free format, namelist construction. FSD

  17. Diffusion in flexible pipes

    Energy Technology Data Exchange (ETDEWEB)

    Brogaard Kristensen, S.

    2000-06-01

    This report describes the work done on modelling and simulation of the complex diffusion of gas through the wall of a flexible pipe. The diffusion and thus the pressure in annulus depends strongly on the diffusion and solubility parameters of the gas-polymer system and on the degree of blocking of the outer surface of the inner liner due to pressure reinforcements. The report evaluates the basis modelling required to describe the complex geometries and flow patterns. Qualitatively results of temperature and concentration profiles are shown in the report. For the program to serve any modelling purpose in 'real life' the results need to be validated and possibly the model needs corrections. Hopefully, a full-scale test of a flexible pipe will provide the required temperatures and pressures in annulus to validate the models. (EHS)

  18. Control of flexible structures

    Science.gov (United States)

    Russell, R. A.

    1985-01-01

    The requirements for future space missions indicate that many of these spacecraft will be large, flexible, and in some applications, require precision geometries. A technology program that addresses the issues associated with the structure/control interactions for these classes of spacecraft is discussed. The goal of the NASA control of flexible structures technology program is to generate a technology data base that will provide the designer with options and approaches to achieve spacecraft performance such as maintaining geometry and/or suppressing undesired spacecraft dynamics. This technology program will define the appropriate combination of analysis, ground testing, and flight testing required to validate the structural/controls analysis and design tools. This work was motivated by a recognition that large minimum weight space structures will be required for many future missions. The tools necessary to support such design included: (1) improved structural analysis; (2) modern control theory; (3) advanced modeling techniques; (4) system identification; and (5) the integration of structures and controls.

  19. Flexible rotor dynamics analysis

    Science.gov (United States)

    Shen, F. A.

    1973-01-01

    A digital computer program was developed to analyze the general nonaxisymmetric and nonsynchronous transient and steady-state rotor dynamic performance of a bending- and shear-wise flexible rotor-bearing system under various operating conditions. The effects of rotor material mechanical hysteresis, rotor torsion flexibility, transverse effects of rotor axial and torsional loading and the anisotropic, in-phase and out-of-phase bearing stiffness and damping force and moment coefficients were included in the program to broaden its capability. An optimum solution method was found and incorporated in the computer program. Computer simulation of experimental data was made and qualitative agreements observed. The mathematical formulations, computer program verification, test data simulation, and user instruction was presented and discussed.

  20. FLEXIBLE GEIGER COUNTER

    Science.gov (United States)

    Richter, H.G.; Gillespie, A.S. Jr.

    1963-11-12

    A flexible Geiger counter constructed from materials composed of vinyl chloride polymerized with plasticizers or co-polymers is presented. The counter can be made either by attaching short segments of corrugated plastic sleeving together, or by starting with a length of vacuum cleaner hose composed of the above materials. The anode is maintained substantially axial Within the sleeving or hose during tube flexing by means of polystyrene spacer disks or an easily assembled polyethylene flexible cage assembly. The cathode is a wire spiraled on the outside of the counter. The sleeving or hose is fitted with glass end-pieces or any other good insulator to maintain the anode wire taut and to admit a counting gas mixture into the counter. Having the cathode wire on the outside of the counter substantially eliminates the objectional sheath effect of prior counters and permits counting rates up to 300,000 counts per minute. (AEC)

  1. Flexible Laser Metal Cutting

    DEFF Research Database (Denmark)

    Villumsen, Sigurd; Jørgensen, Steffen Nordahl; Kristiansen, Morten

    2014-01-01

    This paper describes a new flexible and fast approach to laser cutting called ROBOCUT. Combined with CAD/CAM technology, laser cutting of metal provides the flexibility to perform one-of-a-kind cutting and hereby realises mass production of customised products. Today’s laser cutting techniques...... possess, despite their wide use in industry, limitations regarding speed and geometry. Research trends point towards remote laser cutting techniques which can improve speed and geometrical freedom and hereby the competitiveness of laser cutting compared to fixed-tool-based cutting technology...... such as punching. This paper presents the concepts and preliminary test results of the ROBOCUT laser cutting technology, a technology which potentially can revolutionise laser cutting....

  2. PTFE films with improved flexibility

    Science.gov (United States)

    Muraca, R. F.; Koch, A. A.

    1972-01-01

    Development and application of flexible polytetrafluroethylene films for expulsion bladders in spacecraft propellant tanks are described. Flexibility of material is obtained by reducing crystallinity through annealing and quenching in water. Physical and mechanical properties of material are presented.

  3. Industrial Fuel Flexibility Workshop

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2006-09-01

    On September 28, 2006, in Washington, DC, ITP and Booz Allen Hamilton conducted a fuel flexibility workshop with attendance from various stakeholder groups. Workshop participants included representatives from the petrochemical, refining, food and beverage, steel and metals, pulp and paper, cement and glass manufacturing industries; as well as representatives from industrial boiler manufacturers, technology providers, energy and waste service providers, the federal government and national laboratories, and developers and financiers.

  4. Flexible Land Administration

    DEFF Research Database (Denmark)

    Enemark, Stig

    2014-01-01

    Security of tenure is widely considered to be the missing piece of the puzzle when it comes to eradication of poverty. And, as explained in the previous issue of Geoinformatics, the European Union is now placing land rights at the heart of EU development policy. This article presents a way forwar...... in terms of building flexible and "fit-for-purpose" land administration systems in developing countries. This will ensure security of tenure for all and sustainable management of the use of land....

  5. More flexibility for DESY

    CERN Multimedia

    2003-01-01

    In the future, budgeting at DESY will be more efficient. The 16 associated research centers are to be granted a wider margin in matters of finance and human resources. The Budget Committee of the German Bundestag has agreed to abstain from mandatory staff appointments. The current pre-determined "job pyramid" will be replaced by a more flexible mechanism, so that the conclusion of fixed labor contracts can more closely follow the needs of research (1 page).

  6. Flexibility in Data Management

    OpenAIRE

    Voigt, Hannes

    2014-01-01

    With the ongoing expansion of information technology, new fields of application requiring data management emerge virtually every day. In our knowledge culture increasing amounts of data and work force organized in more creativity-oriented ways also radically change traditional fields of application and question established assumptions about data management. For instance, investigative analytics and agile software development move towards a very agile and flexible handling of data. As the prim...

  7. Construction and Validation of the Rhodobacter sphaeroides 2.4.1 DNA Microarray: Transcriptome Flexibility at Diverse Growth Modes

    Energy Technology Data Exchange (ETDEWEB)

    Pappas, Christopher T. [University of Wyoming, Laramie; Sram, Jakub [University of Wyoming, Laramie; Moskvin, Oleg V. [University of Wyoming, Laramie; Ivanov, Pavel S. [University of Wyoming, Laramie; Mackenzie, Christopher [University of Texas; Choudhary, Madhusudan [University of Texas; Land, Miriam L [ORNL; Larimer, Frank W [ORNL; Kaplan, Samuel [ORNL; Gomelsky, Mark [University of Wyoming, Laramie

    2004-07-01

    A high-density oligonucleotide DNA microarray, a genechip, representing the 4.6-Mb genome of the facultative phototrophic proteobacterium, Rhodobacter sphaeroides 2.4.1, was custom-designed and manufactured by Affymetrix, Santa Clara, Calif. The genechip contains probe sets for 4,292 open reading frames (ORFs), 47 rRNA and tRNA genes, and 394 intergenic regions. The probe set sequences were derived from the genome annotation generated by Oak Ridge National Laboratory after extensive revision, which was based primarily upon codon usage characteristic of this GC-rich bacterium. As a result of the revision, numerous missing ORFs were uncovered, nonexistent ORFs were deleted, and misidentified start codons were corrected. To evaluate R. sphaeroides transcriptome flexibility, expression profiles for three diverse growth modes-aerobic respiration, anaerobic respiration in the dark, and anaerobic photosynthesis-were generated. Expression levels of one-fifth to one-third of the R. sphaeroides ORFs were significantly different in cells under any two growth modes. Pathways involved in energy generation and redox balance maintenance under three growth modes were reconstructed. Expression patterns of genes involved in these pathways mirrored known functional changes, suggesting that massive changes in gene expression are the major means used by R. sphaeroides in adaptation to diverse conditions. Differential expression was observed for genes encoding putative new participants in these pathways (additional photosystem genes, duplicate NADH dehydrogenase, ATP synthases), whose functionality has yet to be investigated. The DNA microarray data correlated well with data derived from quantitative reverse transcription-PCR, as well as with data from the literature, thus validating the R. sphaeroides genechip as a powerful and reliable tool for studying unprecedented metabolic versatility of this bacterium.

  8. Designing structural supply chain flexibility

    NARCIS (Netherlands)

    Mulinski, Ksawery Jan

    2012-01-01

    In a continuously changing business environment the role of supply chain flexibility is constantly increasing. A flexible supply chain can ensure survival in quickly changing market conditions as well as enable sustainable growth. This thesis explores the topic of supply chain flexibility with focus

  9. Flexible Scheduling: Making the Transition

    Science.gov (United States)

    Creighton, Peggy Milam

    2008-01-01

    Citing literature that supports the benefits of flexible scheduling on student achievement, the author exhorts readers to campaign for flexible scheduling in their library media centers. She suggests tips drawn from the work of Graziano (2002), McGregor (2006) and Stripling (1997) for making a smooth transition from fixed to flexible scheduling:…

  10. Measuring and Comparing Energy Flexibilities

    DEFF Research Database (Denmark)

    Valsomatzis, Emmanouil; Hose, Katja; Pedersen, Torben Bach;

    2015-01-01

    Flexibility in energy supply and demand becomes more and more important with increasing Renewable Energy Sources (RES) production and the emergence of the Smart Grid. So-called prosumers, i.e., entities that produce and/or consume energy, can offer their inherent flexibilities through so......-called demand response and thus help stabilize the energy markets. Thus, prosumer flexibility becomes valuable and the ongoing Danish project TotalFlex [1] explores the use of prosumer flexibility in the energy market using the concept of a flex-offer [2], which captures energy flexibilities in time and...... induced by time and amount individually, and by their com- bination. To this end, we introduce several flexibility measures that take into account the combined effect of time and energy on flex-offer flexibility and discuss their respective pros and cons through a number of realistic examples....

  11. Flexible composite radiation detector

    Science.gov (United States)

    Cooke, D. Wayne; Bennett, Bryan L.; Muenchausen, Ross E.; Wrobleski, Debra A.; Orler, Edward B.

    2006-12-05

    A flexible composite scintillator was prepared by mixing fast, bright, dense rare-earth doped powdered oxyorthosilicate (such as LSO:Ce, LSO:Sm, and GSO:Ce) scintillator with a polymer binder. The binder is transparent to the scintillator emission. The composite is seamless and can be made large and in a wide variety of shapes. Importantly, the composite can be tailored to emit light in a spectral region that matches the optimum response of photomultipliers (about 400 nanometers) or photodiodes (about 600 nanometers), which maximizes the overall detector efficiency.

  12. Rotating flexible drag mill

    Science.gov (United States)

    Pepper, W.B.

    1984-05-09

    A rotating parachute for decelerating objects travelling through atmosphere at subsonic or supersonic deployment speeds includes a circular canopy having a plurality of circumferentially arranged flexible panels projecting radially from a solid central disk. A slot extends radially between adjacent panels to the outer periphery of the canopy. Upon deployment, the solid disk diverts air radially to rapidly inflate the panels into a position of maximum diameter. Air impinging on the panels adjacent the panel slots rotates the parachute during its descent. Centrifugal force flattens the canopy into a constant maximum diameter during terminal descent for maximum drag and deceleration.

  13. Rational design, synthesis, and DNA binding properties of novel sequence-selective peptidyl congeners of ametantrone.

    Science.gov (United States)

    Gianoncelli, Alessandra; Basili, Serena; Scalabrin, Matteo; Sosic, Alice; Moro, Stefano; Zagotto, Giuseppe; Palumbo, Manlio; Gresh, Nohad; Gatto, Barbara

    2010-07-01

    Natural and synthetic compounds characterized by an anthraquinone nucleus represent an important class of anti-neoplastic agents, the mechanism of action of which is related to intercalation into DNA. Ametantrone (AM) is a synthetic 9,10-anthracenedione bearing two (hydroxyethylamino)ethylamino residues at positions 1 and 4; along with other anthraquinones and anthracyclines, it shares a polycyclic intercalating moiety and charged side chains that stabilize DNA binding. All these drugs elicit adverse side effects, which represent a challenge for antitumor chemotherapy. In the present work the structure of AM was augmented with appropriate groups that target well-defined base pairs in the major groove. These should endow AM with DNA sequence selectivity. We describe the rationale for the synthesis and the evaluation of activity of a new series of compounds in which the planar anthraquinone is conjugated at positions 1 and 4 through the side chains of AM or other bioisosteric linkers to appropriate dipeptides. The designed novel AM derivatives were shown to selectively stabilize two oligonucleotide duplexes that both have a palindromic GC-rich hexanucleotide core, but their stabilizing effects on a random DNA sequence was negligible. In the case of the most effective compound, the 1,4-bis-[Gly-(L-Lys)] derivative of AM, the experimental results confirm the predictions of earlier theoretical computations. In contrast, AM had equal stabilizing effects on all three sequences and showed no preferential binding. This novel peptide derivative can be classified as a strong binder regarding the sequences that it selectively targets, possibly opening the exploitation of less cytotoxic conjugates of AM to the targeted treatment of oncological and viral diseases. PMID:20458714

  14. Asymmetric Flexible Supercapacitor Stack

    Directory of Open Access Journals (Sweden)

    Leela Mohana Reddy A

    2008-01-01

    Full Text Available AbstractElectrical double layer supercapacitor is very significant in the field of electrical energy storage which can be the solution for the current revolution in the electronic devices like mobile phones, camera flashes which needs flexible and miniaturized energy storage device with all non-aqueous components. The multiwalled carbon nanotubes (MWNTs have been synthesized by catalytic chemical vapor deposition technique over hydrogen decrepitated Mischmetal (Mm based AB3alloy hydride. The polymer dispersed MWNTs have been obtained by insitu polymerization and the metal oxide/MWNTs were synthesized by sol-gel method. Morphological characterizations of polymer dispersed MWNTs have been carried out using scanning electron microscopy (SEM, transmission electron microscopy (TEM and HRTEM. An assymetric double supercapacitor stack has been fabricated using polymer/MWNTs and metal oxide/MWNTs coated over flexible carbon fabric as electrodes and nafion®membrane as a solid electrolyte. Electrochemical performance of the supercapacitor stack has been investigated using cyclic voltammetry, galvanostatic charge-discharge, and electrochemical impedance spectroscopy.

  15. Flexible ocean upwelling pipe

    Science.gov (United States)

    Person, Abraham

    1980-01-01

    In an ocean thermal energy conversion facility, a cold water riser pipe is releasably supported at its upper end by the hull of the floating facility. The pipe is substantially vertical and has its lower end far below the hull above the ocean floor. The pipe is defined essentially entirely of a material which has a modulus of elasticity substantially less than that of steel, e.g., high density polyethylene, so that the pipe is flexible and compliant to rather than resistant to applied bending moments. The position of the lower end of the pipe relative to the hull is stabilized by a weight suspended below the lower end of the pipe on a flexible line. The pipe, apart from the weight, is positively buoyant. If support of the upper end of the pipe is released, the pipe sinks to the ocean floor, but is not damaged as the length of the line between the pipe and the weight is sufficient to allow the buoyant pipe to come to a stop within the line length after the weight contacts the ocean floor, and thereafter to float submerged above the ocean floor while moored to the ocean floor by the weight. The upper end of the pipe, while supported by the hull, communicates to a sump in the hull in which the water level is maintained below the ambient water level. The sump volume is sufficient to keep the pipe full during heaving of the hull, thereby preventing collapse of the pipe.

  16. Chaperone-assisted translocation of flexible polymers in three dimensions

    Science.gov (United States)

    Suhonen, P. M.; Linna, R. P.

    2016-01-01

    Polymer translocation through a nanometer-scale pore assisted by chaperones binding to the polymer is a process encountered in vivo for proteins. Studying the relevant models by computer simulations is computationally demanding. Accordingly, previous studies are either for stiff polymers in three dimensions or flexible polymers in two dimensions. Here, we study chaperone-assisted translocation of flexible polymers in three dimensions using Langevin dynamics. We show that differences in binding mechanisms, more specifically, whether a chaperone can bind to a single site or multiple sites on the polymer, lead to substantial differences in translocation dynamics in three dimensions. We show that the single-binding mode leads to dynamics that is very much like that in the constant-force driven translocation and accordingly mainly determined by tension propagation on the cis side. We obtain β ≈1.26 for the exponent for the scaling of the translocation time with polymer length. This fairly low value can be explained by the additional friction due to binding particles. The multiple-site binding leads to translocation the dynamics of which is mainly determined by the trans side. For this process we obtain β ≈1.36 . This value can be explained by our derivation of β =4 /3 for constant-bias translocation, where translocated polymer segments form a globule on the trans side. Our results pave the way for understanding and utilizing chaperone-assisted translocation where variations in microscopic details lead to rich variations in the emerging dynamics.

  17. Conformation-controlled binding kinetics of antibodies

    Science.gov (United States)

    Galanti, Marta; Fanelli, Duccio; Piazza, Francesco

    2016-01-01

    Antibodies are large, extremely flexible molecules, whose internal dynamics is certainly key to their astounding ability to bind antigens of all sizes, from small hormones to giant viruses. In this paper, we build a shape-based coarse-grained model of IgG molecules and show that it can be used to generate 3D conformations in agreement with single-molecule Cryo-Electron Tomography data. Furthermore, we elaborate a theoretical model that can be solved exactly to compute the binding rate constant of a small antigen to an IgG in a prescribed 3D conformation. Our model shows that the antigen binding process is tightly related to the internal dynamics of the IgG. Our findings pave the way for further investigation of the subtle connection between the dynamics and the function of large, flexible multi-valent molecular machines.

  18. Aggregating and Disaggregating Flexibility Objects

    DEFF Research Database (Denmark)

    Siksnys, Laurynas; Valsomatzis, Emmanouil; Hose, Katja;

    2015-01-01

    In many scientific and commercial domains we encounter flexibility objects, i.e., objects with explicit flexibilities in a time and an amount dimension (e.g., energy or product amount). Applications of flexibility objects require novel and efficient techniques capable of handling large amounts...... of such objects while preserving flexibility. Hence, this paper formally defines the concept of flexibility objects (flex-objects) and provides a novel and efficient solution for aggregating and disaggregating flex-objects. Out of the broad range of possible applications, this paper will focus on smart grid...... energy data management and discuss strategies for aggregation and disaggregation of flex-objects while retaining flexibility. This paper further extends these approaches beyond flex-objects originating from energy consumption by additionally considering flex-objects originating from energy production...

  19. Mobile, Flexible, and Adaptable

    DEFF Research Database (Denmark)

    Agergaard, Jytte; Thao, Thi Vu

    2011-01-01

    Industrialisation and urban growth are constitutive aspects of Vietnam's new economy and are important driving forces behind increasing rural-to-urban migration. Growth in informal sector employment is a significant aspect of this development, which has provided for both male and female migrants...... networking, and remittance practices. The paper is organised around why and how migrants have entered the informal labour market in Hanoi and how they make their livings there while also maintaining ties with their rural homes. In conclusion, we discuss how the migration networks and remittance practices of...... the female porters demonstrate a particular way of adapting to the migration process. Also, it is emphasised how women's flexible practices are facilitated by women's own village-based networks. It is suggested that ‘in-betweenness’, which stands for the simultaneous and overlapping presence of urban...

  20. CANDU fuel cycle flexibility

    International Nuclear Information System (INIS)

    High neutron economy, on-power refuelling, and a simple bundle design provide a high degree of flexibility that enables CANDU (Canada Deuterium Uranium; registered trademark) reactors to be fuelled with a wide variety of fuel types. Near-term applications include the use of slightly enriched uranium (SEU), and recovered uranium (RU) from reprocessed spent Light Water Reactor (LWR) fuel. Plutonium and other actinides arising from various sources, including spent LWR fuel, can be accommodated, and weapons-origin plutonium could be destroyed by burning in CANDU. In the DUPIC fuel cycle, a dry processing method would convert spent Pressurized Water Reactor (PWR) fuel to CANDU fuel. The thorium cycle remains of strategic interest in CANDU to ensure long-term resource availability, and would be of specific interest to those countries possessing large thorium reserves, but limited uranium resources. (author). 21 refs

  1. Synchronization of flexible sheets

    CERN Document Server

    Elfring, Gwynn J; 10.1017/S0022112011000814

    2011-01-01

    When swimming in close proximity, some microorganisms such as spermatozoa synchronize their flagella. Previous work on swimming sheets showed that such synchronization requires a geometrical asymmetry in the flagellar waveforms. Here we inquire about a physical mechanism responsible for such symmetry-breaking in nature. Using a two-dimensional model, we demonstrate that flexible sheets with symmetric internal forcing, deform when interacting with each other via a thin fluid layer in such a way as to systematically break the overall waveform symmetry, thereby always evolving to an in-phase conformation where energy dissipation is minimized. This dynamics is shown to be mathematically equivalent to that obtained for prescribed waveforms in viscoelastic fluids, emphasizing the crucial role of elasticity in symmetry-breaking and synchronization.

  2. Flexible retinal electrode array

    Energy Technology Data Exchange (ETDEWEB)

    Okandan, Murat (Albuquerque, NM); Wessendorf, Kurt O. (Albuquerque, NM); Christenson, Todd R. (Albuquerque, NM)

    2006-10-24

    An electrode array which has applications for neural stimulation and sensing. The electrode array can include a large number of electrodes each of which is flexibly attached to a common substrate using a plurality of springs to allow the electrodes to move independently. The electrode array can be formed from a combination of bulk and surface micromachining, with electrode tips that can include an electroplated metal (e.g. platinum, iridium, gold or titanium) or a metal oxide (e.g. iridium oxide) for biocompatibility. The electrode array can be used to form a part of a neural prosthesis, and is particularly well adapted for use in an implantable retinal prosthesis where the electrodes can be tailored to provide a uniform gentle contact pressure with optional sensing of this contact pressure at one or more of the electrodes.

  3. Conformational flexibility of aspartame.

    Science.gov (United States)

    Toniolo, Claudio; Temussi, Pierandrea

    2016-05-01

    L-Aspartyl-L-phenylalanine methyl ester, better known as aspartame, is not only one of the most used artificial sweeteners, but also a very interesting molecule with respect to the correlation between molecular structure and taste. The extreme conformational flexibility of this dipeptide posed a huge difficulty when researchers tried to use it as a lead compound to design new sweeteners. In particular, it was difficult to take advantage of its molecular model as a mold to infer the shape of the, then unknown, active site of the sweet taste receptor. Here, we follow the story of the 3D structural aspects of aspartame from early conformational studies to recent docking into homology models of the receptor. © 2016 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 106: 376-384, 2016. PMID:27038223

  4. Flexible Language Interoperability

    DEFF Research Database (Denmark)

    Ekman, Torbjörn; Mechlenborg, Peter; Schultz, Ulrik Pagh

    2007-01-01

    Virtual machines raise the abstraction level of the execution environment at the cost of restricting the set of supported languages. Moreover, the ability of a language implementation to integrate with other languages hosted on the same virtual machine typically constrains the features of the lan...... the Smalltalk object model, which provides interoperability for embedded versions of the Smalltalk, Java, and BETA programming languages....... language. In this paper, we present a highly flexible yet efficient approach to hosting multiple programming languages on an object-oriented virtual machine. Our approach is based on extending the interface of each class with language-specific wrapper methods, offering each language a tailored view of a......Virtual machines raise the abstraction level of the execution environment at the cost of restricting the set of supported languages. Moreover, the ability of a language implementation to integrate with other languages hosted on the same virtual machine typically constrains the features of the...

  5. Scarring, habituation and job flexibility

    OpenAIRE

    Green, Colin; Leeves, Gareth

    2009-01-01

    Increases in the use of flexible employment contracts create more frequent transitions between unemployment and employment. This paper analyses the impact of cumulative unemployment experiences on the life satisfaction of Australian male workers in flexible employment. Using panel data techniques, it was found that permanent contract workers were scarred by previous unemployment. This contrasted with flexible contract workers who seem habituated to the effects of past unemployment. Social nor...

  6. Probabilistic Inference of Transcription Factor Binding from Multiple Data Sources

    OpenAIRE

    Lähdesmäki, Harri; Rust, Alistair G.; Shmulevich, Ilya

    2008-01-01

    An important problem in molecular biology is to build a complete understanding of transcriptional regulatory processes in the cell. We have developed a flexible, probabilistic framework to predict TF binding from multiple data sources that differs from the standard hypothesis testing (scanning) methods in several ways. Our probabilistic modeling framework estimates the probability of binding and, thus, naturally reflects our degree of belief in binding. Probabilistic modeling also allows for ...

  7. Flexible ureterorenoscopy: Tips and tricks

    Directory of Open Access Journals (Sweden)

    Bhaskar Kumar Somani

    2013-01-01

    Full Text Available With advancement in technology, improvement in endoscope and ancillary equipment, more complex procedures can be performed using flexible ureterorenoscopy. In this review article we provide a summary of flexible ureterorenoscopic procedures with "tips and tricks′′ for success for each type of procedure. It looks at the disposables used with flexible ureterorenoscopic procedures, set up and patient positioning for gaining access, insertion and handling of scope and the use of urethral access sheath. We also provide techniques for various flexible ureterorenoscopic procedures including management of renal stones, calyceal diverticula and upper tract urothelial tumours.

  8. Promoter analysis of intestinal genes induced during iron-deprivation reveals enrichment of conserved SP1-like binding sites

    Directory of Open Access Journals (Sweden)

    Hu Zihua

    2007-11-01

    Full Text Available Abstract Background Iron-deficiency leads to the induction of genes related to intestinal iron absorption and homeostasis. By analyzing a large GeneChip® dataset from the rat intestine, we identified a large cluster of 228 genes that was induced by iron-deprivation. Only 2 of these genes contained 3' iron-response elements, suggesting that other regulation including transcriptional may be involved. We therefore utilized computational methods to test the hypothesis that some of the genes within this large up-regulated cluster are co-ordinately regulated by common transcriptional mechanisms. We thus identified promoters from the up-regulated gene cluster from rat, mouse and human, and performed enrichment analyses with the Clover program and the TRANSFAC database. Results Surprisingly, we found a strong statistical enrichment for SP1 binding sites in our experimental promoters as compared to background sequences. As the TRANSFAC database cannot distinguish among SP/KLF family members, many of which bind similar GC-rich DNA sequences, we surmise that SP1 or an SP1-like factor could be involved in this response. In fact, we detected induction of SP6/KLF14 in the GeneChip® studies, and confirmed it by real-time PCR. Additional computational analyses suggested that an SP1-like factor may function synergistically with a FOX TF to regulate a subset of these genes. Furthermore, analysis of promoter sequences identified many genes with multiple, conserved SP1 and FOX binding sites, the relative location of which within orthologous promoters was highly conserved. Conclusion SP1 or a closely related factor may play a primary role in the genetic response to iron-deficiency in the mammalian intestine.

  9. On flexible and rigid nouns

    DEFF Research Database (Denmark)

    Rijkhoff, Jan

    This article argues that in addition to the major flexible lexical categories in Hengeveld’s classification of parts of speech systems (Contentive, Non-Verb, Modifier), there are also flexible word classes within the rigid lexical category Noun (Set Noun, Sort Noun, General Noun). Members of flex...... of speech hierarchy....

  10. Building Highly Flexible Polyelectrolyte Nanotubes

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ Flexibility of polyelectrolyte nanotubes is necessary if they are to be exploited in applications such as developing photoelectric devices with strong mechanical properties. In a recent attempt, high flexibility has been observed from such nanotubes prepared by a research team headed by Prof. Li Junbai of the CAS Institute of Chemistry (ICCAS).

  11. Teacher Flexibility in Mathematical Discussion

    Science.gov (United States)

    Leikin, Roza; Dinur, Sariga

    2007-01-01

    The significance of discussion in mathematics classes has been prominently debated in the research literature. Different studies have stressed the importance of teacher flexibility in orchestrating the discussion. We introduce an operational definition of teacher flexibility. In a case study with one secondary-school mathematics teacher, we…

  12. Global Analysis of Flexible Risers

    DEFF Research Database (Denmark)

    Banke, Lars

    1996-01-01

    thus resists the loading from the environment in a more suitable way.A flexible pipe is a composite structure, built up of several layers each having a specific function. Due to the flexibility, the response of the pipe in wave induced motions are governed by a complex hydroelastic set of differential...... equations.An important part in the design of flexible pipes is the end fitting. The purpose of the end fitting is mainly to connect pipe units, but it also combines and terminates the different layers of the flexible pipe. The end fitting system contains a bending stiffener and an end fitting. The bending...... stiffener is installed to prevent damages caused by bending concentrations at the connection. The bending stiffener is designed to optimise the curvature distribution from the flexible riser to the vessel and to minimise the support forces at the vessel.Today, when end-fitting system are designed FEM...

  13. [The flexibility of family medicine].

    Science.gov (United States)

    Minguet, C; Aubrege, A; Aubart, M; Cornuz, J; di Patrizio, P; Du Boullay, D; Farghadani, H; Flammang, M; Haas, N; Kacenelenbogen, N; Kopp, M; Leners, J C; Levêque, M; Mbengue, M; Paur, H; Paur, I; Raphaël, F; Rausch, S; Shetgen, M; Stein R; Tabouring, P; Thomas, J M; Vignon, G

    2015-01-01

    We are a European academic group of family doctors and we propose a definition of flexibility in family medicine. A review of the literature shows that flexibility and complexity are emerging concepts in the field of family practice. The outcomes of a workshop at the WONCA-Europe congress in 2014 are discussed. The flexibility is a capability of the general practitioner to deal with complex clinical situations in a biomedical and societal changing world. Flexibility is framed by ethics. It could improve the quality of care, be useful against burnout and used in medical research. In conclusion, family medicine should adopt a specific definition of the flexibility describing its specificity, a useful and teachable capacity. PMID:26946851

  14. Flexible Foot Test Assembly

    Energy Technology Data Exchange (ETDEWEB)

    Kurita, C.H.; /Fermilab

    1987-04-27

    A test model of the flexible foot support was constructed early in the design stages to check its reactions to applied loads. The prototype was made of SS 304 and contained four vertical plates as opposed to the fourteen Inconel 718 plates which comprise the actual structure. Due to the fact that the prototype was built before the design of the support was finalized, the plate dimensions are different from those of the actual proposed design (i.e. model plate thickness is approximately one-half that of the actual plates). See DWG. 3740.210-MC-222376 for assembly details of the test model and DWG. 3740.210-MB-222377 for plate dimensions. This stanchion will be required to not only support the load of the inner vessel of the cryostat and its contents, but it must also allow for the movement of the vessel due to thermal contraction. Assuming that each vertical plate acts as a column, then the following formula from the Manual of Steel Construction (American Institute of Steel Construction, Inc., Eigth edition, 1980) can be applied to determine whether or not such columns undergoing simultaneous axial compression and transverse loading are considered safe for the given loading. The first term is representative of the axially compressive stress, and the second term, the bending stress. If the actual compressive stress is greater than 15% of the allowable compressive stress, then there are additional considerations which must be accounted for in the bending stress term.

  15. Probabilistic Model to Treat Flexibility in Molecular Contacts

    OpenAIRE

    Hakulinen, Riku; Puranen, Santeri

    2016-01-01

    Evaluating accessible conformational space is computationally expensive and thermal motions are partly neglected in computer models of molecular interactions. This produces error into the estimates of binding strength. We introduce a method for modelling interactions so that structural flexibility is inherently taken into account. It has a statistical model for 3D properties of 'nonlocal' contacts and a physics based description of 'local' interactions, based on mechanical torque. The form of...

  16. Rolling-cuff flexible bellows

    Science.gov (United States)

    Lambert, D.R.

    1982-09-27

    A flexible connector apparatus used to join two stiff non-deformable members, such as piping, is described. The apparatus is provided with one or more flexible sections or assemblies each utilizing a bellows of a rolling cuff type connected between two ridge members, with the bellows being supported by a back-up ring, such that only the curved end sections of the bellows are unsupported. Thus, the bellows can be considered as being of a tube-shaped configuration and thus have high pressure resistance. The components of the flexible apparatus are sealed or welded one to another such that it is fluid tight.

  17. Flexible sensors for biomedical technology.

    Science.gov (United States)

    Vilela, Diana; Romeo, Agostino; Sánchez, Samuel

    2016-02-01

    Flexible sensing devices have gained a great deal of attention among the scientific community in recent years. The application of flexible sensors spans over several fields, including medicine, industrial automation, robotics, security, and human-machine interfacing. In particular, non-invasive health-monitoring devices are expected to play a key role in the improvement of patient life and in reducing costs associated with clinical and biomedical diagnostic procedures. Here, we focus on recent advances achieved in flexible devices applied on the human skin for biomedical and healthcare purposes. PMID:26675174

  18. Displays develop a new flexibility

    Directory of Open Access Journals (Sweden)

    Jin Jang

    2006-04-01

    Full Text Available Flexible displays are of great interest especially for mobile applications. Currently, there are no active-matrix flexible displays on the market, even though research has been carried out continuously over several years. Here, we introduce flexible displays and the prototype devices that have been developed. Organic thin-film transistors have been fabricated on plastic substrates for display backplanes. We describe the performance of our transistors made using a self-organization process. Finally, a set of research goals are outlined.

  19. Flexibility of trunnion piping elbows

    International Nuclear Information System (INIS)

    Flexibility factors and stress indices for piping component such as straight pipe, elbows, butt-welding tees, branch connections, and butt-welding reducers are contained in the code, but many of the less common piping components, like the trunnion elbow, do not have flexibility factors or stress indices defined. The purpose of this paper is to identify the in-plane and out-of-plane flexibility factors in accordance with code procedures for welded trunnions attached to the tangent centerlines of long radius elbows. This work utilized the finite element method as applicable to plates and shells for calculating the relative rotations of the trunnion elbow-ends for in-plane and out-of-plane elbow moment loadings. These rotations are used to derive the corresponding in-plane and out-of-plane flexibility factors. (orig./GL)

  20. On flexible and rigid nouns

    DEFF Research Database (Denmark)

    Rijkhoff, Jan

    2008-01-01

    Studies in Language 32-3 (2008), 727-752. Special issue: Parts of Speech: Descriptive tools, theoretical constructs Jan Rijkhoff - On flexible and rigid nouns This article argues that in addition to the flexible lexical categories in Hengeveld’s classification of parts-of-speech systems (Contenti...... classifications of parts-of-speech systems, (b) correlates with certain grammatical phenomena (e.g. so called number discord), and (c) helps to explain the parts-of-speech hierarchy....

  1. Time-Precision Flexible Adder

    OpenAIRE

    García Chamizo, Juan Manuel; Mora Pascual, Jerónimo Manuel; Mora Mora, Higinio

    2003-01-01

    A new conception of flexible calculation that allows us to adjust a sum depending on the available time computation is presented. More specifically, the objective is to obtain a calculation model that makes the processing time/precision more flexible. The addition method is based on carry-select scheme adder and the proposed design uses precalculated data stored in look-up tables, which provide, above all, quality results and systematization in the implementation of low level primitives that ...

  2. Developmental constraints on behavioural flexibility

    OpenAIRE

    Holekamp, Kay E.; Swanson, Eli M.; Van Meter, Page E.

    2013-01-01

    We suggest that variation in mammalian behavioural flexibility not accounted for by current socioecological models may be explained in part by developmental constraints. From our own work, we provide examples of constraints affecting variation in behavioural flexibility, not only among individuals, but also among species and higher taxonomic units. We first implicate organizational maternal effects of androgens in shaping individual differences in aggressive behaviour emitted by female spotte...

  3. Determinants of flexible work arrangements

    OpenAIRE

    Sarbu, Miruna

    2014-01-01

    Flexible work arrangements such as allowing employees to work at home are used in firms, especially since information and communication technologies have become so widespread. Using individual-level data from 10,884 German employees, this paper analyses the determinants of working at home as a form of flexible work arrangements. The analysis is based on descriptive analyses and a discrete choice model using a probit estimation approach. The results reveal that men have a higher...

  4. Option values and flexibility preference

    OpenAIRE

    Strazzera, Elisabetta

    1998-01-01

    Preference for flexibility is a behavioral attitude displayed by people that prefer reversible to irreversible actions, and that are willing to pay a premium in order to maintain the possibility of changing their decision. This paper provides a functional characterization of preference for flexibility, based on the notion of option value. The proposed theory is shown to be useful to explain the success of marketing policies that guarantee reimbursement in consumption goods and financial marke...

  5. A structure-based model for predicting serum albumin binding.

    Directory of Open Access Journals (Sweden)

    Katrina W Lexa

    Full Text Available One of the many factors involved in determining the distribution and metabolism of a compound is the strength of its binding to human serum albumin. While experimental and QSAR approaches for determining binding to albumin exist, various factors limit their ability to provide accurate binding affinity for novel compounds. Thus, to complement the existing tools, we have developed a structure-based model of serum albumin binding. Our approach for predicting binding incorporated the inherent flexibility and promiscuity known to exist for albumin. We found that a weighted combination of the predicted logP and docking score most accurately distinguished between binders and nonbinders. This model was successfully used to predict serum albumin binding in a large test set of therapeutics that had experimental binding data.

  6. Chaperone-assisted translocation of flexible polymers in three dimensions.

    Science.gov (United States)

    Suhonen, P M; Linna, R P

    2016-01-01

    Polymer translocation through a nanometer-scale pore assisted by chaperones binding to the polymer is a process encountered in vivo for proteins. Studying the relevant models by computer simulations is computationally demanding. Accordingly, previous studies are either for stiff polymers in three dimensions or flexible polymers in two dimensions. Here, we study chaperone-assisted translocation of flexible polymers in three dimensions using Langevin dynamics. We show that differences in binding mechanisms, more specifically, whether a chaperone can bind to a single site or multiple sites on the polymer, lead to substantial differences in translocation dynamics in three dimensions. We show that the single-binding mode leads to dynamics that is very much like that in the constant-force driven translocation and accordingly mainly determined by tension propagation on the cis side. We obtain β≈1.26 for the exponent for the scaling of the translocation time with polymer length. This fairly low value can be explained by the additional friction due to binding particles. The multiple-site binding leads to translocation the dynamics of which is mainly determined by the trans side. For this process we obtain β≈1.36. This value can be explained by our derivation of β=4/3 for constant-bias translocation, where translocated polymer segments form a globule on the trans side. Our results pave the way for understanding and utilizing chaperone-assisted translocation where variations in microscopic details lead to rich variations in the emerging dynamics. PMID:26871100

  7. Balancing energy flexibilities through aggregation

    DEFF Research Database (Denmark)

    Valsomatzis, Emmanouil; Hose, Katja; Pedersen, Torben Bach

    2014-01-01

    in both energy production and consumption, is the key to solving these problems. Flexibilities can be expressed as flex-offers, which due to their high number need to be aggregated to reduce the complexity of energy scheduling. In this paper, we discuss balance aggregation techniques that already......One of the main goals of recent developments in the Smart Grid area is to increase the use of renewable energy sources. These sources are characterized by energy fluctuations that might lead to energy imbalances and congestions in the electricity grid. Exploiting inherent flexibilities, which exist...... during aggregation aim at balancing flexibilities in production and consumption to reduce the probability of congestions and reduce the complexity of scheduling. We present results of our extensive experiments....

  8. The flexible model and its shell

    International Nuclear Information System (INIS)

    The flexible model is a new type of model that can be used for describing complex systems including both numerical computation and symbolical operation. The idea of flexible modeling was presented according to the concept, principle, and method of Intelligent Engineering. This paper will first introduce the flexible model and the method of flexible modeling. Then, a kind of flexible model shell will be presented

  9. Polymer electronics a flexible technology

    CERN Document Server

    Technology, Rapra

    2009-01-01

    The worldwide market for polymer electronic products has been estimated to be worth up to £15 billion by 2015 and the opportunity for new markets could be as high as £125 billion by 2025.'The rapid development of polymer electronics has revealed the possibility for transforming the electronics market by offering lighter, flexible and more cost effective alternatives to conventional materials and products. With applications ranging from printed, flexible conductors and novel semiconductor components to intelligent labels and large area displays and solar panels, products that were previously un

  10. Flexible graphene based microwave attenuators.

    Science.gov (United States)

    Byun, Kisik; Ju Park, Yong; Ahn, Jong-Hyun; Min, Byung-Wook

    2015-02-01

    We demonstrate flexible 3 dB and 6 dB microwave attenuators using multilayer graphene grown by the chemical vapor deposition method. On the basis of the characterized results of multilayer graphene and graphene-Au ohmic contacts, the graphene attenuators are designed and measured. The flexible graphene-based attenuators have 3 dB and 6 dB attenuation with a return loss of less than -15 dB at higher than 5 GHz. The devices have shown durability in a bending cycling test of 100 times. The circuit model of the attenuator based on the characterized results matches the experimental results well. PMID:25590144

  11. Flexible graphene based microwave attenuators

    International Nuclear Information System (INIS)

    We demonstrate flexible 3 dB and 6 dB microwave attenuators using multilayer graphene grown by the chemical vapor deposition method. On the basis of the characterized results of multilayer graphene and graphene–Au ohmic contacts, the graphene attenuators are designed and measured. The flexible graphene-based attenuators have 3 dB and 6 dB attenuation with a return loss of less than −15 dB at higher than 5 GHz. The devices have shown durability in a bending cycling test of 100 times. The circuit model of the attenuator based on the characterized results matches the experimental results well. (paper)

  12. Dictionary-Symbolwise Flexible Parsing

    OpenAIRE

    Crochemore, Maxime; Giambruno, Laura; Langiu, Alessio; Mignosi, Filippo; Restivo, Antonio

    2011-01-01

    Linear time optimal parsing algorithms are very rare in the dictionary based branch of the data compression theory. The most recent is the Flexible Parsing algorithm of Mathias and Shainalp that works when the dictionary is prefix closed and the encoding of dictionary pointers has a constant cost. We present the Dictionary-Symbolwise Flexible Parsing algorithm that is optimal for prefix-closed dictionaries and any symbolwise compressor under some natural hypothesis. In the case of LZ78-alike al...

  13. Architecture of cognitive flexibility revealed by lesion mapping.

    Science.gov (United States)

    Barbey, Aron K; Colom, Roberto; Grafman, Jordan

    2013-11-15

    Neuroscience has made remarkable progress in understanding the architecture of human intelligence, identifying a distributed network of brain structures that support goal-directed, intelligent behavior. However, the neural foundations of cognitive flexibility and adaptive aspects of intellectual function remain to be well characterized. Here, we report a human lesion study (n=149) that investigates the neural bases of key competencies of cognitive flexibility (i.e., mental flexibility and the fluent generation of new ideas) and systematically examine their contributions to a broad spectrum of cognitive and social processes, including psychometric intelligence (Wechsler Adult Intelligence Scale), emotional intelligence (Mayer, Salovey, Caruso Emotional Intelligence Test), and personality (Neuroticism-Extraversion-Openness Personality Inventory). Latent variable modeling was applied to obtain error-free indices of each factor, followed by voxel-based lesion-symptom mapping to elucidate their neural substrates. Regression analyses revealed that latent scores for psychometric intelligence reliably predict latent scores for cognitive flexibility (adjusted R(2)=0.94). Lesion mapping results further indicated that these convergent processes depend on a shared network of frontal, temporal, and parietal regions, including white matter association tracts, which bind these areas into an integrated system. A targeted analysis of the unique variance explained by cognitive flexibility further revealed selective damage within the right superior temporal gyrus, a region known to support insight and the recognition of novel semantic relations. The observed findings motivate an integrative framework for understanding the neural foundations of adaptive behavior, suggesting that core elements of cognitive flexibility emerge from a distributed network of brain regions that support specific competencies for human intelligence. PMID:23721727

  14. Spectroscopic studies of the binding of Cu(II) complexes of oxicam NSAIDs to alternating G-C and homopolymeric G-C sequences

    Science.gov (United States)

    Chakraborty, Sreeja; Bose, Madhuparna; Sarkar, Munna

    2014-03-01

    Drugs belonging to the Non-steroidal anti-inflammatory (NSAID) group are not only used as anti-inflammatory, analgesic and anti-pyretic agents, but also show anti-cancer effects. Complexing them with a bioactive metal like copper, show an enhancement in their anti-cancer effects compared to the bare drugs, whose exact mechanism of action is not yet fully understood. For the first time, it was shown by our group that Cu(II)-NSAIDs can directly bind to the DNA backbone. The ability of the copper complexes of NSAIDs namely meloxicam and piroxicam to bind to the DNA backbone could be a possible molecular mechanism behind their enhanced anticancer effects. Elucidating base sequence specific interaction of Cu(II)-NSAIDs to the DNA will provide information on their possible binding sites in the genome sequence. In this work, we present how these complexes respond to differences in structure and hydration pattern of GC rich sequences. For this, binding studies of Cu(II) complexes of piroxicam [Cu(II)-(Px)2 (L)2] and meloxicam [Cu(II)-(Mx)2 (L)] with alternating GC (polydG-dC) and homopolymeric GC (polydG-polydC) sequences were carried out using a combination of spectroscopic techniques that include UV-Vis absorption, fluorescence and circular dichroism (CD) spectroscopy. The Cu(II)-NSAIDs show strong binding affinity to both polydG-dC and polydG-polydC. The role reversal of Cu(II)-meloxicam from a strong binder of polydG-dC (Kb = 11.5 × 103 M-1) to a weak binder of polydG-polydC (Kb = 5.02 × 103 M-1), while Cu(II)-piroxicam changes from a strong binder of polydG-polydC (Kb = 8.18 × 103 M-1) to a weak one of polydG-dC (Kb = 2.18 × 103 M-1), point to the sensitivity of these complexes to changes in the backbone structures/hydration. Changes in the profiles of UV absorption band and CD difference spectra, upon complex binding to polynucleotides and the results of competitive binding assay using ethidium bromide (EtBr) fluorescence indicate different binding modes in each

  15. Protein flexibility in the light of structural alphabets

    Directory of Open Access Journals (Sweden)

    Pierrick eCraveur

    2015-05-01

    Full Text Available Protein structures are valuable tools to understand protein function. Nonetheless, proteins are often considered as rigid macromolecules while their structures exhibit specific flexibility, which is essential to complete their functions. Analyses of protein structures and dynamics are often performed with a simplified three-state description, i.e. the classical secondary structures. . More precise and complete description of protein backbone conformation can be obtained using libraries of small protein fragments that are able to approximate every part of protein structures. These libraries, called structural alphabets (SAs, have been widely used in structure analysis field, from definition of ligand binding sites to superimposition of protein structures. SAs are also well suited to analyze the dynamics of protein structures.Here, we review innovative approaches that investigate protein flexibility based on SAs description. Coupled to various sources of experimental data (e.g. B-factor and computational methodology (e.g. Molecular Dynamic simulation, SAs turn out to be powerful tools to analyze protein dynamics, e.g. to examine allosteric mechanisms in large set of structures in complexes, to identify order/disorder transition. SAs were also shown to be quite efficient to predict protein flexibility from amino-acid sequence. Finally, in this review, we exemplify the interest of SAs for studying flexibility with different cases of proteins implicated in pathologies and diseases.

  16. Hydrophobic Core Flexibility Modulates Enzyme Activity in HIV-1 Protease

    Energy Technology Data Exchange (ETDEWEB)

    Mittal, Seema; Cai, Yufeng; Nalam, Madhavi N.L.; Bolon, Daniel N.A.; Schiffer, Celia A. (UMASS, MED)

    2012-09-11

    Human immunodeficiency virus Type-1 (HIV-1) protease is crucial for viral maturation and infectivity. Studies of protease dynamics suggest that the rearrangement of the hydrophobic core is essential for enzyme activity. Many mutations in the hydrophobic core are also associated with drug resistance and may modulate the core flexibility. To test the role of flexibility in protease activity, pairs of cysteines were introduced at the interfaces of flexible regions remote from the active site. Disulfide bond formation was confirmed by crystal structures and by alkylation of free cysteines and mass spectrometry. Oxidized and reduced crystal structures of these variants show the overall structure of the protease is retained. However, cross-linking the cysteines led to drastic loss in enzyme activity, which was regained upon reducing the disulfide cross-links. Molecular dynamics simulations showed that altered dynamics propagated throughout the enzyme from the engineered disulfide. Thus, altered flexibility within the hydrophobic core can modulate HIV-1 protease activity, supporting the hypothesis that drug resistant mutations distal from the active site can alter the balance between substrate turnover and inhibitor binding by modulating enzyme activity.

  17. Protein flexibility in the light of structural alphabets.

    Science.gov (United States)

    Craveur, Pierrick; Joseph, Agnel P; Esque, Jeremy; Narwani, Tarun J; Noël, Floriane; Shinada, Nicolas; Goguet, Matthieu; Leonard, Sylvain; Poulain, Pierre; Bertrand, Olivier; Faure, Guilhem; Rebehmed, Joseph; Ghozlane, Amine; Swapna, Lakshmipuram S; Bhaskara, Ramachandra M; Barnoud, Jonathan; Téletchéa, Stéphane; Jallu, Vincent; Cerny, Jiri; Schneider, Bohdan; Etchebest, Catherine; Srinivasan, Narayanaswamy; Gelly, Jean-Christophe; de Brevern, Alexandre G

    2015-01-01

    Protein structures are valuable tools to understand protein function. Nonetheless, proteins are often considered as rigid macromolecules while their structures exhibit specific flexibility, which is essential to complete their functions. Analyses of protein structures and dynamics are often performed with a simplified three-state description, i.e., the classical secondary structures. More precise and complete description of protein backbone conformation can be obtained using libraries of small protein fragments that are able to approximate every part of protein structures. These libraries, called structural alphabets (SAs), have been widely used in structure analysis field, from definition of ligand binding sites to superimposition of protein structures. SAs are also well suited to analyze the dynamics of protein structures. Here, we review innovative approaches that investigate protein flexibility based on SAs description. Coupled to various sources of experimental data (e.g., B-factor) and computational methodology (e.g., Molecular Dynamic simulation), SAs turn out to be powerful tools to analyze protein dynamics, e.g., to examine allosteric mechanisms in large set of structures in complexes, to identify order/disorder transition. SAs were also shown to be quite efficient to predict protein flexibility from amino-acid sequence. Finally, in this review, we exemplify the interest of SAs for studying flexibility with different cases of proteins implicated in pathologies and diseases. PMID:26075209

  18. Flexible Learning: A Luddite View

    Science.gov (United States)

    Baggaley, Jon

    2011-01-01

    This article reflects on the flexible learning concept through the eyes of the 19th-century industrial activists known as the Luddites. During a period of economic uncertainty, the Luddite perspective provides a sensitive justification for a change-free educational environment, and for a backlash in favour of "inflexible learning" (IL). The…

  19. Flexible Query Answering Systems 2006

    DEFF Research Database (Denmark)

    -computer interaction. The overall theme of the FQAS conferences is innovative query systems aimed at providing easy, flexible, and intuitive access to information. Such systems are intended to facilitate retrieval from information repositories such as databases, libraries, and the World-Wide Web. These repositories...

  20. Easy and flexible mixture distributions

    DEFF Research Database (Denmark)

    Fosgerau, Mogens; Mabit, Stefan L.

    2013-01-01

    We propose a method to generate flexible mixture distributions that are useful for estimating models such as the mixed logit model using simulation. The method is easy to implement, yet it can approximate essentially any mixture distribution. We test it with good results in a simulation study and...

  1. Pipelined Flexible Krylov Subspace Methods

    Science.gov (United States)

    Sanan, Patrick; Schnepp, Sascha M.; May, Dave A.

    2015-04-01

    State-of-the-art geophysical forward models expend most of their computational resources solving large, sparse linear systems. To date, preconditioned Krylov subspace methods have proven to be the only algorithmically scalable approach to solving these systems. However, at `extreme scale', the global reductions required by the inner products within these algorithms become a computational bottleneck, and it becomes advantageous to use pipelined Krylov subspace methods. These allow overlap of global reductions with other work, at the expense of using more storage and local computational effort, including overhead required to synchronize overlapping work. An impediment to using currently-available pipelined solvers for relevant geophysical forward modeling is that they are not `flexible', meaning that they cannot support nonlinear or varying preconditioners. Such preconditioners are effective for solving challenging linear systems, notably those arising from modelling of Stokes flow with highly heterogeneous viscosity structure. To this end, we introduce, for the first time, Krylov subspace methods which are both pipelined and flexible. We implement and demonstrate pipelined, flexible Conjugate Gradient, GMRES, and Conjugate Residual methods, which will be made publicly available via the open source PETSc library. Our algorithms are nontrivial modifications of the flexible methods they are based on (that is, they are not equivalent in exact arithmetic), so we analyze them mathematically and through a number of numerical experiments employing multi-level preconditioners. We highlight the benefits of these algorithms by solving variable viscosity Stokes problems directly relevant to lithospheric dynamics.

  2. Flexibility in Language and Thought

    Science.gov (United States)

    Srinivasan, Mahesh

    2011-01-01

    Many words can be used flexibly: a book can be physically heavy, but also have provocative content, a "chicken" might live in a coop, but could also be tasty to eat. What do the uses of "polysemous" words such as these reveal about the structure of language and thought? Paper 1 examined 4-year-old children's representations of polysemous words…

  3. Flexible Bistable Cholesteric Reflective Displays

    Science.gov (United States)

    Yang, Deng-Ke

    2006-03-01

    Cholesteric liquid crystals (ChLCs) exhibit two stable states at zero field condition-the reflecting planar state and the nonreflecting focal conic state. ChLCs are an excellent candidate for inexpensive and rugged electronic books and papers. This paper will review the display cell structure,materials and drive schemes for flexible bistable cholesteric (Ch) reflective displays.

  4. Flexible Schedules and Shift Work.

    Science.gov (United States)

    Beers, Thomas M.

    2000-01-01

    Flexible work hours have gained prominence, as more than 25 million workers (27.6% of all full-time workers) can now vary their schedules. However, there has been little change since the mid-1980s in the proportion who work a shift other than a regular daytime shift. (JOW)

  5. Functional aspects of protein flexibility

    DEFF Research Database (Denmark)

    Teilum, Kaare; Olsen, Johan G; Kragelund, Birthe B

    2009-01-01

    Proteins are dynamic entities, and they possess an inherent flexibility that allows them to function through molecular interactions within the cell, among cells and even between organisms. Appreciation of the non-static nature of proteins is emerging, but to describe and incorporate this into an ...

  6. Drug Promiscuity in PDB: Protein Binding Site Similarity Is Key.

    Directory of Open Access Journals (Sweden)

    V Joachim Haupt

    Full Text Available Drug repositioning applies established drugs to new disease indications with increasing success. A pre-requisite for drug repurposing is drug promiscuity (polypharmacology - a drug's ability to bind to several targets. There is a long standing debate on the reasons for drug promiscuity. Based on large compound screens, hydrophobicity and molecular weight have been suggested as key reasons. However, the results are sometimes contradictory and leave space for further analysis. Protein structures offer a structural dimension to explain promiscuity: Can a drug bind multiple targets because the drug is flexible or because the targets are structurally similar or even share similar binding sites? We present a systematic study of drug promiscuity based on structural data of PDB target proteins with a set of 164 promiscuous drugs. We show that there is no correlation between the degree of promiscuity and ligand properties such as hydrophobicity or molecular weight but a weak correlation to conformational flexibility. However, we do find a correlation between promiscuity and structural similarity as well as binding site similarity of protein targets. In particular, 71% of the drugs have at least two targets with similar binding sites. In order to overcome issues in detection of remotely similar binding sites, we employed a score for binding site similarity: LigandRMSD measures the similarity of the aligned ligands and uncovers remote local similarities in proteins. It can be applied to arbitrary structural binding site alignments. Three representative examples, namely the anti-cancer drug methotrexate, the natural product quercetin and the anti-diabetic drug acarbose are discussed in detail. Our findings suggest that global structural and binding site similarity play a more important role to explain the observed drug promiscuity in the PDB than physicochemical drug properties like hydrophobicity or molecular weight. Additionally, we find ligand

  7. The next generation of transcription factor binding site prediction.

    Directory of Open Access Journals (Sweden)

    Anthony Mathelier

    Full Text Available Finding where transcription factors (TFs bind to the DNA is of key importance to decipher gene regulation at a transcriptional level. Classically, computational prediction of TF binding sites (TFBSs is based on basic position weight matrices (PWMs which quantitatively score binding motifs based on the observed nucleotide patterns in a set of TFBSs for the corresponding TF. Such models make the strong assumption that each nucleotide participates independently in the corresponding DNA-protein interaction and do not account for flexible length motifs. We introduce transcription factor flexible models (TFFMs to represent TF binding properties. Based on hidden Markov models, TFFMs are flexible, and can model both position interdependence within TFBSs and variable length motifs within a single dedicated framework. The availability of thousands of experimentally validated DNA-TF interaction sequences from ChIP-seq allows for the generation of models that perform as well as PWMs for stereotypical TFs and can improve performance for TFs with flexible binding characteristics. We present a new graphical representation of the motifs that convey properties of position interdependence. TFFMs have been assessed on ChIP-seq data sets coming from the ENCODE project, revealing that they can perform better than both PWMs and the dinucleotide weight matrix extension in discriminating ChIP-seq from background sequences. Under the assumption that ChIP-seq signal values are correlated with the affinity of the TF-DNA binding, we find that TFFM scores correlate with ChIP-seq peak signals. Moreover, using available TF-DNA affinity measurements for the Max TF, we demonstrate that TFFMs constructed from ChIP-seq data correlate with published experimentally measured DNA-binding affinities. Finally, TFFMs allow for the straightforward computation of an integrated TF occupancy score across a sequence. These results demonstrate the capacity of TFFMs to accurately model DNA

  8. Total iron binding capacity

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/003489.htm Total iron binding capacity To use the sharing features on this page, please enable JavaScript. Total iron binding capacity (TIBC) is a blood test to ...

  9. Plant Hormone Binding Sites

    OpenAIRE

    Napier, Richard

    2004-01-01

    • Aims Receptors for plant hormones are becoming identified with increasing rapidity, although a frustrating number remain unknown. There have also been many more hormone‐binding proteins described than receptors. This Botanical Briefing summarizes what has been discovered about hormone binding sites, their discovery and descriptions, and will not dwell on receptor functions or activities except where these are relevant to understand binding.

  10. Flexible Temperature Sensors on Fibers

    Directory of Open Access Journals (Sweden)

    Marcin Sloma

    2010-08-01

    Full Text Available The aim of this paper is to present research dedicated to the elaboration of novel, miniaturized flexible temperature sensors for textronic applications. Examined sensors were manufactured on a single yarn, which ensures their high flexibility and good compatibility with textiles. Stable and linear characteristics were obtained by special technological process and applied temperature profiles. As a thermo-sensitive materials the innovative polymer compositions filled with multiwalled carbon nanotubes were used. Elaborated material was adapted to printing and dip-coating techniques to produce NTC composites. Nanotube sensors were free from tensometric effect typical for other carbon-polymer sensor, and demonstrated TCR of 0.13%/K. Obtained temperature sensors, compatible with textile structure, can be applied in rapidly developing smart textiles and be used for health and protections purposes.

  11. Flexible Adaptation in Cognitive Radios

    CERN Document Server

    Li, Shujun

    2013-01-01

    This book provides an introduction to software-defined radio and cognitive radio, along with methodologies for applying knowledge representation, semantic web, logic reasoning and artificial intelligence to cognitive radio, enabling autonomous adaptation and flexible signaling. Readers from the wireless communications and software-defined radio communities will use this book as a reference to extend software-defined radio to cognitive radio, using the semantic technology described. Readers with a background in semantic web and artificial intelligence will find in this book the application of semantic web and artificial intelligence technologies to wireless communications. For readers in networks and network management, this book presents a new approach to enable interoperability, collaborative optimization and flexible adaptation of network components. Provides a comprehensive ontology covering the core concepts of wireless communications using a formal language; Presents the technical realization of using a ...

  12. Lost and Found in Flexibility

    DEFF Research Database (Denmark)

    Bødker, Susanne; Christiansen, Ellen

    2002-01-01

    'New work' is characterized by mobility and flexibility and a growing need to facilitate social awareness. The paper discusses how to augment the environment with social awareness and suggest 'awareness-in-transition' as a lable for awareness in mobile work in flexible work environments, and as...... locus for building social awareness in the 'new work'. A transition (e.g. taking information from a whiteboard in a meeting room to the desktop of a personal computer)--besides the immediate implications at the functional level--has important interpersonal, intra-personal, and organizational...... implications for the building of a social awareness among the collaborators, because it builds on recognition of difference in the environment. Building on Grudin's 'Eight Challenges for the Developers' of Computer Supported Cooperative Work, through analysis of a scenario and two experiments based on case...

  13. Thin, Flexible IMM Solar Array

    Science.gov (United States)

    Walmsley, Nicholas

    2015-01-01

    NASA needs solar arrays that are thin, flexible, and highly efficient; package compactly for launch; and deploy into large, structurally stable high-power generators. Inverted metamorphic multijunction (IMM) solar cells can enable these arrays, but integration of this thin crystalline cell technology presents certain challenges. The Thin Hybrid Interconnected Solar Array (THINS) technology allows robust and reliable integration of IMM cells into a flexible blanket comprising standardized modules engineered for easy production. The modules support the IMM cell by using multifunctional materials for structural stability, shielding, coefficient of thermal expansion (CTE) stress relief, and integrated thermal and electrical functions. The design approach includes total encapsulation, which benefits high voltage as well as electrostatic performance.

  14. WAGE FLEXIBILITY IN THE CONTEMPORARY SOCIETY

    Directory of Open Access Journals (Sweden)

    TECULESCU Silviu Alexandru

    2010-12-01

    Full Text Available The paper follows to offer the most efficient solutions for the attainment by Romania of the economic development level associated to the Western European countries. It proposes the division of the flexibility of labour market in three components, namely: internal flexibility, external flexibility and wage flexibility. The analysis performed within the present study will emphasize the wage flexibility. Wage flexibility can be classified in four components: a plans of individual and group incentives; b plans of assigning wages out of productivity; c plans of distribution of profits and, respectively, d plans of suggestions. The labour market flexibility, in general, and especially the wage flexibility contributes to the increase of employee motivation at the workplace, aspect which leeds to the growth of labour productivity, through this one being put the bases of the medium- and long-term economic development.

  15. ULTRA BARRIER TOPSHEET (UBT) FOR FLEXIBLE PHOTOVOLTAICS

    Energy Technology Data Exchange (ETDEWEB)

    DeScioli, Derek

    2013-06-01

    This slide-show presents 3M photovoltaic-related products, particularly flexible components. Emphasis is on the 3M Ultra Barrier Solar Films. Topics covered include reliability and qualification testing and flexible photovoltaic encapsulation costs.

  16. Understanding flexible and distributed software development processes

    OpenAIRE

    Agerfalk, Par J.; Fitzgerald, Brian

    2006-01-01

    peer-reviewed The minitrack on Flexible and Distributed Software Development Processes addresses two important and partially intertwined current themes in software development: process flexibility and globally distributed software development

  17. Electromagnetic forces in flexible systems

    OpenAIRE

    Hong, Jia-Sheng; Allen, John Elliston; Dr. J.E. Allen

    1994-01-01

    Some problems in electro dynamics have been studied in the light of electromagnetic field theory and computational techniques. One is that of the retrograde motion of the electric arc – an old and intriguing problem of physics. A more complete model based on electrodynamics has been proposed for study of this problem. In this model, the plasma column is treated as a flexible conductor. The associated 3-dimensional boundary-value problem was solved by the boundary element me...

  18. Smart Tendon Actuated Flexible Actuator

    OpenAIRE

    Md. Masum Billah; Raisuddin Khan

    2015-01-01

    We investigate the kinematic feasibility of a tendon-based flexible parallel platform actuator. Much of the research on tendon-driven Stewart platforms is devoted either to the completely restrained positioning mechanism (CRPM) or to one particular type of the incompletely restrained positioning mechanism (IRPM) where the external force is provided by the gravitational pull on the platform such as in cable-suspended Stewart platforms. An IRPM-based platform is proposed which uses the external...

  19. Flexible Seal Accommodates Part Mismatch

    Science.gov (United States)

    Bobb, I.

    1983-01-01

    Chain of plates embedded in flexible seal enables it to withstand side loading of 2,300 psi (116MPa) while sealing gap of up to 0.5 inch (13 mm) between cylindrical chamber wall and test fixture. Pressure-actuated seal along inner edge forces seal into contact even though cylinder wall becomes eccentric as cylinder pressure increases. Seal has many industrial applications, particularly where heat or pressure causes distortion of chamber being sealed.

  20. CRISIS FOCUS Uncertainty and Flexibility

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    As the world continued to watch the unfolding financial and economic crises this month, Robert Zoellick, President of the World Bank, arrived in China for discussions on how the country can help support the global economy and the efforts it has taken to strengthen its own recovery. Zoellick, who had seen many uncertainties in 2009, called for China to be flexible with its macroeconomic policy. He made the following comments at a press conference in Beijing on December 15. Edited excerpts follow:

  1. Has compensation become more flexible?

    OpenAIRE

    Sandra A. Cannon; Bruce C. Fallick; Michael Lettau; Raven E. Saks

    2000-01-01

    In recent years, numerous observers have argued that global competition, increased reliance on contingent workers, and the breakdown of implicit contracts have made compensation practices in the United States more flexible; in particular, employers have become more concerned with how an employee's pay compares to that in other firms and less concerned with considerations of equity or relative pay within the firm. This paper uses establishment-level data from the Bureau of Labor Statistics' Em...

  2. Thermodynamics of tubelike flexible polymers

    OpenAIRE

    Vogel, T.; Neuhaus, T; Bachmann, M.; Janke, W.

    2009-01-01

    In this work, we discuss the general phase behavior of short tubelike flexible polymers. The geometric thickness constraint is implemented through the concept of the global radius of curvature. We use sophisticated Monte Carlo sampling methods to simulate small bead-stick polymer models with Lennard-Jones interaction among nonbonded monomers. We analyze energetic fluctuations and structural quantities to classify conformational pseudophases. We find that the tube thickness influences the ther...

  3. Flexible propulsors in ground effect

    International Nuclear Information System (INIS)

    We present experimental evidence for the hydrodynamic benefits of swimming ‘in ground effect’, that is, near a solid boundary. This situation is common to fish that swim near the substrate, especially those that are dorsoventrally compressed, such as batoids and flatfishes. To investigate flexible propulsors in ground effect, we conduct force measurements and particle image velocimetry on flexible rectangular panels actuated at their leading edge near the wall of a water channel. For a given actuation mode, the panels swim faster near the channel wall while maintaining the same propulsive economy. In conditions producing net thrust, panels produce more thrust near the ground. When operating in resonance, swimming near the ground can also increase propulsive efficiency. Finally, the ground can act to suppress three-dimensional modes, thereby increasing thrust and propulsive efficiency. The planform considered here is non-biological, but the hydrodynamic benefits are likely to apply to more complex geometries, especially those where broad flexible propulsors are involved such as fish bodies and fins. Such fish could produce more thrust by swimming near the ground, and in some cases do so more efficiently. (paper)

  4. Understanding flexibility of a software organization

    OpenAIRE

    Nurdiani, Indira

    2015-01-01

    Context: Flexibility is an important capability for a software organization. Without flexibility a software organization risks losing its competitive advantage. To build software organization flexibility every constituent of the organization need to be taken into account. Otherwise there are unforeseen trade-offs that could have negative impacts on the rest of the organization. However, currently known flexibility approaches, such as, Agile and Lean methodologies are currently implemented at ...

  5. Flexible Thermoelectric Device for Localized Thermal Management

    OpenAIRE

    Lee, Minjae

    2016-01-01

    Flexible thermoelectric (TE) refrigerators are attractive for personalized thermal management, for example, when integrated into clothes. Flexible TE power generators can also find applications in wearable self-powered mobile electronics. These devices, however, need high thermoelectric performance and mechanical flexibility under compressive and tensile strains, a combination of device properties that are often difficult to achieve. Despite significant efforts in the field, flexible TE devic...

  6. 21 CFR 868.5530 - Flexible laryngoscope.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Flexible laryngoscope. 868.5530 Section 868.5530...) MEDICAL DEVICES ANESTHESIOLOGY DEVICES Therapeutic Devices § 868.5530 Flexible laryngoscope. (a) Identification. A flexible laryngoscope is a fiberoptic device used to examine and visualize a patient's...

  7. Towards Measuring Investment in Flexible Foundry Manufacturing

    OpenAIRE

    Rhythm Suren Wadhwa

    2012-01-01

    Manufacturing flexibility is an important instrument to ensure the success of manufacturing systems in the modern day competitive and uncertain environment. The major hindrance in integrating flexibility into decision making process is that it is difficult to measure and be compared to future indefinable manufacturing scenarios. This paper presents a methodical concept utilizing real options to evaluate flexible foundry manufacturing system.

  8. Flexible Work Options within the Organisational System

    Science.gov (United States)

    Albion, Majella J.; Chee, Munli

    2006-01-01

    The availability of flexible work options provides an opportunity for individuals to shape their careers in order to optimise their work and life goals. This study takes a systems theory approach to examine how the use of flexible work options influences relationships and interactions in the workplace. The "Flexible Work Options Questionnaire"…

  9. Financial Flexibility in North Carolina Schools.

    Science.gov (United States)

    Suarez, Tanya M.; Polen, Deborah A.

    This paper explores educational financial flexibility with a focus on the specific issues surrounding local flexibility in North Carolina school districts. Strategies that states have used to increase local financial flexibility include waivers, reduction of budget categories, block grants, and school-based budgeting. The North Carolina system of…

  10. Towards Measuring Investment in Flexible Foundry Manufacturing

    Directory of Open Access Journals (Sweden)

    Rhythm Suren Wadhwa

    2012-07-01

    Full Text Available Manufacturing flexibility is an important instrument to ensure the success of manufacturing systems in the modern day competitive and uncertain environment. The major hindrance in integrating flexibility into decision making process is that it is difficult to measure and be compared to future indefinable manufacturing scenarios. This paper presents a methodical concept utilizing real options to evaluate flexible foundry manufacturing system.

  11. Structure-based druggability assessment of the mammalian structural proteome with inclusion of light protein flexibility.

    Directory of Open Access Journals (Sweden)

    Kathryn A Loving

    2014-07-01

    Full Text Available Advances reported over the last few years and the increasing availability of protein crystal structure data have greatly improved structure-based druggability approaches. However, in practice, nearly all druggability estimation methods are applied to protein crystal structures as rigid proteins, with protein flexibility often not directly addressed. The inclusion of protein flexibility is important in correctly identifying the druggability of pockets that would be missed by methods based solely on the rigid crystal structure. These include cryptic pockets and flexible pockets often found at protein-protein interaction interfaces. Here, we apply an approach that uses protein modeling in concert with druggability estimation to account for light protein backbone movement and protein side-chain flexibility in protein binding sites. We assess the advantages and limitations of this approach on widely-used protein druggability sets. Applying the approach to all mammalian protein crystal structures in the PDB results in identification of 69 proteins with potential druggable cryptic pockets.

  12. Coenzyme A Binding to the Aminoglycoside Acetyltransferase (3)-IIIb Increases Conformational Sampling of Antibiotic Binding Site

    Energy Technology Data Exchange (ETDEWEB)

    Hu, Xiaohu [ORNL; Norris, Adrianne [University of Tennessee, Knoxville (UTK); Baudry, Jerome Y [ORNL; Serpersu, Engin H [University of Tennessee, Knoxville (UTK)

    2011-01-01

    NMR spectroscopy experiments and molecular dynamics simulations were performed to describe the dynamic properties of the aminoglycoside acetyltransferase (3)-IIIb (AAC) in its apo and coenzyme A (CoASH) bound forms. The {sup 15}N-{sup 1}H HSQC spectra indicate a partial structural change and coupling of the CoASH binding site with another region in the protein upon the CoASH titration into the apo enzyme. Molecular dynamics simulations indicate a significant structural and dynamic variation of the long loop in the antibiotic binding domain in the form of a relatively slow (250 ns), concerted opening motion in the CoASH enzyme complex and that binding of the CoASH increases the structural flexibility of the loop, leading to an interchange between several similar equally populated conformations.

  13. Analysis of binding behavior between dynamic structures of a papain and the peptide consisting of 10 GLY residues

    Science.gov (United States)

    Nishiyama, Katsuhiko

    2010-07-01

    The binding of 10GLY to dynamic structures of papain was investigated by molecular dynamics and docking simulations. The binding free energies and sites were greatly fluctuated depending on the time and the binding was more stable and existed at the near site of active center when the structural changes in the highly flexible residues in papain were larger. Binding stability and sites would be significantly influenced by the highly flexible residues. Analysis of such residues would provide an important guideline for clarification of enzymatic activities and modification of structural dynamics of such residues would allow us to control enzymatic activities.

  14. Consumer flexibility - State and challenges

    International Nuclear Information System (INIS)

    This report summarises experiences and results from relevant projects carried out within the area of consumer flexibility in the last years. A general description of the projects is presented. In addition are testing activities, economical signals for motivating the end user for load reductions and appropriate technology for communication and effect management mentioned separately. Briefly summarised is the following achieved: 1) The potential for effect regulating measures in ordinary consumption and in power demanding industry is analysed and estimated to 1750 MW and 3360 MW respectively. 2) Time varied rates are developed and are tested. The NVE has decided on this basis that all the network owners should offer season varied rates to their customers. 3) Test projects in Drammen, Oslo and Trondheim have shown that it is possible to motivate smaller consumers for and implement load reductions. 4) Technology for load management for the end user is evaluated. 5) A survey is made viewing the technology for two way communication. In addition the ''option'' market for the reserves of the Statnett has proved that a steady compensation is a sufficient incentive for producing substantial effect reserves in the load sector (power intensive industry). In the last part of the report the incentives and frame conditions for establishing technical solutions and infrastructure are discussed. Further work will focus on the consumer flexibility in shortage situations. The main challenges are connected to: 1) Establishing a foundation for decisions concerning cost efficient investments in necessary technical equipment. 2) To find an optimal combination of a price flexible and remote controlled load reduction

  15. Cognitive Flexibility in Primary Dystonia.

    Science.gov (United States)

    Lange, Florian; Seer, Caroline; Dengler, Reinhard; Dressler, Dirk; Kopp, Bruno

    2016-07-01

    Objectives Although primary dystonia is typically characterized as a movement disorder, it is also associated with cognitive alterations in the domain of executive functioning which may arise from changes in cortico-basal ganglia circuits. Specifically, in comparison to healthy controls, patients with dystonia show deficits in neuropsychological tests of cognitive flexibility. However, it is unclear whether cognitive inflexibility is caused by the pathomechanisms underlying primary dystonia or by confounding factors such as depression or symptom-related distraction.Methods The present study aimed to eliminate these confounds by examining cognitive flexibility in dystonia patients and in patients with similar motor symptoms but without a comparable central pathophysiology. Eighteen patients with primary blepharospasm, a common form of dystonia affecting the muscles around the eyes, and 19 patients with hemifacial spasm, a facial nerve disorder causing similar eyelid spasms, completed a computerized version of the Wisconsin Card Sorting Test (cWCST). The two groups were further compared on tests of global cognitive functioning, psychiatric symptoms, health status, and impulsiveness. Results Blepharospasm patients committed significantly more errors on the cWCST than patients with hemifacial spasm. Group differences were most pronounced with regard to integration errors, a measure of rule-inference processes on the cWCST. Integration errors were also associated with impulsiveness in patients with blepharospasm. Conclusions Primary blepharospasm is related to deficits in cognitive flexibility, even when blepharospasm patients are compared with patients who suffer from motor symptoms of non-dystonic origin. Our results support the possibility that cognitive inflexibility results from the specific pathophysiological processes underlying primary dystonia. (JINS, 2016, 22, 662-670). PMID:27333537

  16. Flexible wind turbines. Flexibele molens

    Energy Technology Data Exchange (ETDEWEB)

    van der Veld, P.J.

    1983-01-01

    Wind turbines are subject to strong and suddenly fluctuating forces, on special parts of the construction (blade root, axis, hub, mast). This requires sophisticated concepts and special, preferable fibrous, materials. Flexible materials and hingeing constructions and vibration-breaking systems are used widely. Some remarkable concepts are to be mentioned. In some cases the mast as a whole is movable and even turnable around a vertical axis, thus absorbing dangerous forces and also permitting the nacelle to be fixed to the mast. Rotors are designed where the blades are hingeing not at or very near the hub, but at a certain distance from it.

  17. International Expansion through Flexible Replication

    DEFF Research Database (Denmark)

    Jonsson, Anna; Foss, Nicolai Juul

    2011-01-01

    aimed at frequent modification of the format for replication. Another finding is that IKEA treats replication as hierarchical: lower-level features (marketing efforts, pricing, etc.) are allowed to vary across IKEA stores in response to market-based learning, while higher-level features (fundamental...... values, vision, etc.) are replicated in a uniform manner across stores, and change only very slowly (if at all) in response to learning (“flexible replication”). We conclude by discussing the factors that influence the approach to replication adopted by an international replicator....

  18. Flexible spintronic devices on Kapton

    DEFF Research Database (Denmark)

    Bedoya-Pinto, Amilcar; Donolato, Marco; Gobbi, Marco;

    2014-01-01

    Magnetic tunnel junctions and nano-sized domain-wall conduits have been fabricated on the flexible substrate Kapton. Despite the delicate nature of tunneling barriers and zig-zag shaped nanowires, the devices show an outstanding integrity and robustness upon mechanical bending. High values of...... bending angle (r = 5 mm) have been achieved without degradation of the device performance, reaching room-temperature tunneling magnetoresistance ratios of 12% in bended Co/Al2O3/NiFe junctions. In addition, a suitable route to pattern high-quality nanostructures directly on the polyimide surface is...

  19. Flexible varieties and automorphism groups

    CERN Document Server

    Arzhantsev, I; Kaliman, S; Kutzschebauch, F; Zaidenberg, M

    2010-01-01

    Given an affine algebraic variety X of dimension at least 2, we let SAut (X) denote the special automorphism group of X i.e., the subgroup of the full automorphism group Aut (X) generated by all one-parameter unipotent subgroups. We show that if SAut (X) is transitive on the smooth locus of X then it is infinitely transitive on this locus. In turn, the transitivity is equivalent to the flexibility of X. The latter means that for every smooth point x of X the tangent space at x is spanned by the velocity vectors of one-parameter unipotent subgroups of Aut (X). We provide also different variations and applications.

  20. Flexible varieties and automorphism groups

    OpenAIRE

    Arzhantsev, I.; Flenner, H.; Kaliman, S.; Kutzschebauch, F.; Zaidenberg, M

    2013-01-01

    Given an irreducible affine algebraic variety $X$ of dimension $n\\ge2$ , we let $\\operatorname {SAut}(X)$ denote the special automorphism group of $X$ , that is, the subgroup of the full automorphism group $\\operatorname{Aut}(X)$ generated by all one-parameter unipotent subgroups. We show that if $\\operatorname {SAut}(X)$ is transitive on the smooth locus $X_{\\mathrm{reg}}$ , then it is infinitely transitive on $X_{\\mathrm{reg}}$ . In turn, the transitivity is equivalent to the flexibility of...

  1. Lifelong Open and Flexible Learning

    DEFF Research Database (Denmark)

    Bang, Jørgen

    2006-01-01

    EADTU members are universities that are dedicated to off-campus target groups using distance teaching methods as well as associations consisting of universities with mainly mainstream on-campus students but also offering education for off-campus students. Consequently our focus is on Lifelong Open...... and Flexible (LOF) learning embracing characteristics as: open learning, distance learning, e-learning, online learning, open accessibility, multimedia support, virtual mobility, learning communities, dual mode (earn & learn) approaches, and the like.In my presentation I will focus on the EADTU...

  2. Protein flexibility oriented virtual screening strategy for JAK2 inhibitors

    Science.gov (United States)

    Xiong, Xiao; Yuan, Haoliang; Zhang, Yanmin; Xu, Jinxing; Ran, Ting; Liu, Haichun; Lu, Shuai; Xu, Anyang; Li, Hongmei; Jiang, Yulei; Lu, Tao; Chen, Yadong

    2015-10-01

    JAK2 has been considered as an important target for the development of anti-cancer agents. In this study, considering the flexibility of its binding site, an integrated strategy combining Bayesian categorization modeling and ensemble docking was established. Four representative crystal structures were selected for ensemble docking by the hierarchical clustering of 34 crystal structures according to the volume overlaps of each structure. A retrospective virtual screening was performed to validate this integrated strategy. As the preliminary filtration, the Bayesian model enhanced the ratio of actives by reducing the large amount of decoys. After docking the remaining compounds, the comparison between the ensemble and individual results showed that the enrichment of ensemble docking improved significantly. The results of analysis on conformational changes of two top ranked active inhibitors when docking into different proteins indicated that compounds with flexible conformations well fitted the different binding site shapes were more likely to be potential JAK2 inhibitors. This high efficient strategy will facilitate virtual screening for novel JAK2 inhibitors and could be even applied in drug discovery against other targets.

  3. Python bindings for libcloudph++

    OpenAIRE

    Jarecka, Dorota; Arabas, Sylwester; Del Vento, Davide

    2015-01-01

    This technical note introduces the Python bindings for libcloudph++. The libcloudph++ is a C++ library of algorithms for representing atmospheric cloud microphysics in numerical models. The bindings expose the complete functionality of the library to the Python users. The bindings are implemented using the Boost.Python C++ library and use NumPy arrays. This note includes listings with Python scripts exemplifying the use of selected library components. An example solution for using the Python ...

  4. Flexible climate agreements after 2012

    International Nuclear Information System (INIS)

    The Kyoto agreement is only a small step towards much stronger and broader commitments and new creativity is needed to further develop a really global climate policy. A flexible approach is necessary to obtain broad participation and substantial reduction of the emissions of greenhouse gases. Flexibility is also important to ease negotiations, to ensure cost-effectiveness and implement a global climate agreement. The US withdrawal from the Kyoto Protocol has rendered the agreement much less effective than the original goal of five per cent reduction of the emission from the industrialized countries. In addition the emissions are increasing much faster in countries that have not committed themselves to the agreement. The agreement runs out in 2012 and should be followed by a new agreement, the negotiations about which are to start up no later than 2005. Attempts by the European Union to begin a discussion about future commitments were very quickly wrecked by the G77 group with strong support from the U.S.A. To formulate a practical climate policy the general goal in the Climate Convention must be interpreted and specified. It may seem impossible to agree upon a long-term goal. But the clarity it provides will be very useful. It will be a guide for short-term goals and a reference for evaluation of success

  5. DNS & Bind Cookbook

    CERN Document Server

    Liu, Cricket

    2011-01-01

    The DNS & BIND Cookbook presents solutions to the many problems faced by network administrators responsible for a name server. Following O'Reilly's popular problem-and-solution cookbook format, this title is an indispensable companion to DNS & BIND, 4th Edition, the definitive guide to the critical task of name server administration. The cookbook contains dozens of code recipes showing solutions to everyday problems, ranging from simple questions, like, "How do I get BIND?" to more advanced topics like providing name service for IPv6 addresses. It's full of BIND configuration files that yo

  6. Python bindings for libcloudph++

    CERN Document Server

    Jarecka, Dorota; Del Vento, Davide

    2015-01-01

    This technical note introduces the Python bindings for libcloudph++. The libcloudph++ is a C++ library of algorithms for representing atmospheric cloud microphysics in numerical models. The bindings expose the complete functionality of the library to the Python users. The bindings are implemented using the Boost.Python C++ library and use NumPy arrays. This note includes listings with Python scripts exemplifying the use of selected library components. An example solution for using the Python bindings to access libcloudph++ from Fortran is presented.

  7. Flexible network wireless transceiver and flexible network telemetry transceiver

    Science.gov (United States)

    Brown, Kenneth D.

    2008-08-05

    A transceiver for facilitating two-way wireless communication between a baseband application and other nodes in a wireless network, wherein the transceiver provides baseband communication networking and necessary configuration and control functions along with transmitter, receiver, and antenna functions to enable the wireless communication. More specifically, the transceiver provides a long-range wireless duplex communication node or channel between the baseband application, which is associated with a mobile or fixed space, air, water, or ground vehicle or other platform, and other nodes in the wireless network or grid. The transceiver broadly comprises a communication processor; a flexible telemetry transceiver including a receiver and a transmitter; a power conversion and regulation mechanism; a diplexer; and a phased array antenna system, wherein these various components and certain subcomponents thereof may be separately enclosed and distributable relative to the other components and subcomponents.

  8. Massively parallel decoding of mammalian regulatory sequences supports a flexible organizational model.

    Science.gov (United States)

    Smith, Robin P; Taher, Leila; Patwardhan, Rupali P; Kim, Mee J; Inoue, Fumitaka; Shendure, Jay; Ovcharenko, Ivan; Ahituv, Nadav

    2013-09-01

    Despite continual progress in the cataloging of vertebrate regulatory elements, little is known about their organization and regulatory architecture. Here we describe a massively parallel experiment to systematically test the impact of copy number, spacing, combination and order of transcription factor binding sites on gene expression. A complex library of ∼5,000 synthetic regulatory elements containing patterns from 12 liver-specific transcription factor binding sites was assayed in mice and in HepG2 cells. We find that certain transcription factors act as direct drivers of gene expression in homotypic clusters of binding sites, independent of spacing between sites, whereas others function only synergistically. Heterotypic enhancers are stronger than their homotypic analogs and favor specific transcription factor binding site combinations, mimicking putative native enhancers. Exhaustive testing of binding site permutations suggests that there is flexibility in binding site order. Our findings provide quantitative support for a flexible model of regulatory element activity and suggest a framework for the design of synthetic tissue-specific enhancers. PMID:23892608

  9. Ligand photo-isomerization triggers conformational changes in iGluR2 ligand binding domain.

    Directory of Open Access Journals (Sweden)

    Tino Wolter

    Full Text Available Neurological glutamate receptors bind a variety of artificial ligands, both agonistic and antagonistic, in addition to glutamate. Studying their small molecule binding properties increases our understanding of the central nervous system and a variety of associated pathologies. The large, oligomeric multidomain membrane protein contains a large and flexible ligand binding domains which undergoes large conformational changes upon binding different ligands. A recent application of glutamate receptors is their activation or inhibition via photo-switchable ligands, making them key systems in the emerging field of optochemical genetics. In this work, we present a theoretical study on the binding mode and complex stability of a novel photo-switchable ligand, ATA-3, which reversibly binds to glutamate receptors ligand binding domains (LBDs. We propose two possible binding modes for this ligand based on flexible ligand docking calculations and show one of them to be analogues to the binding mode of a similar ligand, 2-BnTetAMPA. In long MD simulations, it was observed that transitions between both binding poses involve breaking and reforming the T686-E402 protein hydrogen bond. Simulating the ligand photo-isomerization process shows that the two possible configurations of the ligand azo-group have markedly different complex stabilities and equilibrium binding modes. A strong but slow protein response is observed after ligand configuration changes. This provides a microscopic foundation for the observed difference in ligand activity upon light-switching.

  10. Structures of apo IRF-3 and IRF-7 DNA binding domains: effect of loop L1 on DNA binding

    Energy Technology Data Exchange (ETDEWEB)

    De Ioannes, Pablo; Escalante, Carlos R.; Aggarwal, Aneel K. (VCU); (Mount Sinai Hospital)

    2013-11-20

    Interferon regulatory factors IRF-3 and IRF-7 are transcription factors essential in the activation of interferon-{beta} (IFN-{beta}) gene in response to viral infections. Although, both proteins recognize the same consensus IRF binding site AANNGAAA, they have distinct DNA binding preferences for sites in vivo. The X-ray structures of IRF-3 and IRF-7 DNA binding domains (DBDs) bound to IFN-{beta} promoter elements revealed flexibility in the loops (L1-L3) and the residues that make contacts with the target sequence. To characterize the conformational changes that occur on DNA binding and how they differ between IRF family members, we have solved the X-ray structures of IRF-3 and IRF-7 DBDs in the absence of DNA. We found that loop L1, carrying the conserved histidine that interacts with the DNA minor groove, is disordered in apo IRF-3 but is ordered in apo IRF-7. This is reflected in differences in DNA binding affinities when the conserved histidine in loop L1 is mutated to alanine in the two proteins. The stability of loop L1 in IRF-7 derives from a unique combination of hydrophobic residues that pack against the protein core. Together, our data show that differences in flexibility of loop L1 are an important determinant of differential IRF-DNA binding.

  11. Inhibition of plasminogen activator inhibitor-1 binding to endocytosis receptors of the low density lipoprotein receptor family by a peptide isolated from a phage displayed library

    DEFF Research Database (Denmark)

    Jensen, Jan K.; Malmendal, Anders; Schiøtt, Birgit;

    2006-01-01

    (DVPCFGWCQDA) was determined by NMR. A binding site in the so-called flexible joint region of PAI-1 was suggested by molecular modelling and validated through binding studies with various competitors and site-directed mutagenesis of PAI-1. The peptide with an N-terminal biotin inhibited the binding of the u...

  12. Cognitive Flexibility in Obsessive Compulsive Disorder

    DEFF Research Database (Denmark)

    Jónsson, Hjalti; Salkovskis, Paul M.

    BT for problems such as OCD requires a level of cognitive flexibility (that is the ability to take a different perspective on ones problems). It could be argued that problems in set shifting (by neuropsychological tests) might underpin problems in this area. Two assessments were used (1: perception...... of cognitive flexibility was assessed by questionnaire 2: neuropsychological evaluation of set shifting). This study will recruit three groups: OCD patients, anxious and healthy controls. Cognitive flexibility is measured using modified version of the Cognitive Flexibility Scale (Martin & Rubin, 1995......) and neuropsychological measures of cognitive flexibility (Wisconsin Cart Sorting Test, Trail Making Test A/B, The Brixton Test). IN addition to the group comparison, the relationship between perceived flexibility, set shifting and psychopathology will be investigated. The implications of the findings...

  13. Melanin-binding radiopharmaceuticals

    Energy Technology Data Exchange (ETDEWEB)

    Packer, S; Fairchild, R G; Watts, K P; Greenberg, D; Hannon, S J

    1980-01-01

    The scope of this paper is limited to an analysis of the factors that are important to the relationship of radiopharmaceuticals to melanin. While the authors do not attempt to deal with differences between melanin-binding vs. melanoma-binding, a notable variance is assumed. (PSB)

  14. Melanin-binding radiopharmaceuticals

    International Nuclear Information System (INIS)

    The scope of this paper is limited to an analysis of the factors that are important to the relationship of radiopharmaceuticals to melanin. While the authors do not attempt to deal with differences between melanin-binding vs. melanoma-binding, a notable variance is assumed

  15. DNS BIND Server Configuration

    Directory of Open Access Journals (Sweden)

    Radu MARSANU

    2011-01-01

    Full Text Available After a brief presentation of the DNS and BIND standard for Unix platforms, the paper presents an application which has a principal objective, the configuring of the DNS BIND 9 server. The general objectives of the application are presented, follow by the description of the details of designing the program.

  16. Long Range Correlation of Hydrophilicity and Flexibility Along the Hemoglobin Chain

    International Nuclear Information System (INIS)

    Within this study, we reveal the long range correlation concerning hydrophilicity and flexibility along sequences of hemoglobins belonging to different organisms and we compare them with the long range correlations properties obtained for other protein families. For all hemoglobins considered, we investigate two discrete spatial series: the hydrophilicity and flexibility respectively. We apply the nonlinear analysis methods to analyze the two spatial series by calculating the spectral coefficient β, the scaling exponent α and Hurst exponent H. The obtained values for the mentioned coefficients suggest long range correlation within the analyzed sequences of hemoglobins in good agreement with those obtained for the calcium binding proteins and hydrolases.

  17. Retrofitting for fossil fuel flexibility

    International Nuclear Information System (INIS)

    Described in this paper are two fossil plant retrofits recently completed by the Public Service Company of New Hampshire that demonstrate the type of planning and execution required for a successful project under the current regulatory and budget constraints. Merrimack Units 1 and 2 are 120 MW and 338 MW nominal cyclone-fired coal units in Bow, New Hampshire. The retrofits recently completed at these plants have resulted in improved particulate emissions compliance, and the fuel flexibility to allow switching to lower sulphur coals to meet current and future SO2 emission limits. Included in this discussion are the features of each project including the unique precipitator procurement approach for the Unit 1 Retrofit, and methods used to accomplish both retrofits within existing scheduled maintenance outages through careful planning and scheduling, effective use of pre-outage construction, 3-D CADD modeling, modular construction and early procurement. Operating experience while firing various coals in the cyclone fired boilers is also discussed

  18. Flexibility of trunnion piping elbows

    International Nuclear Information System (INIS)

    Pipe support designs in nuclear power plants are often dictated by the obvious constraints such as piping loads, space restrictions, and available materials for fabrication. In certain instances, the piping stress analysis may require the placement of a support or anchor on a pipe fitting such as a tee or elbow. In the petrochemical industry, it is not uncommon to have supports attached to an elbow by means of a trunnion, particularly in the close proximity of equipment nozzles. The purpose of this work is to extend the previous analyses to define the in-plane and out-of-plane flexibility factors that are necessary to allow for greater use of the trunnion elbow attachment in the nuclear industry

  19. Flexibility of Protein Secondary Structures

    Science.gov (United States)

    Emberly, Eldon; Mukhopadhyay, Ranjan; Tang, Chao; Wingreen, Ned

    2004-03-01

    Proteins consist of compact packings of secondary structural elements: helices and sheets comprised of strands. The deformations of these structures often play an important role in determining the final shape of the protein (i.e. coiled coils or beta-barrels). We have performed a principal component analysis on secondary structural elements extracted from the protein structure database to determine their flexible modes. For helices we find only two dominant internal modes: bend and twist. Sheets also only display two dominant modes: in-sheet bend and a spiral twist. We report on the scaling of these modes with secondary element size. For helices, the dynamic normal modes of a simple spring model are able to recapitulate the modes and scalings found. For sheets, we find different scaling behaviors for parallel and anti-parallel sheets and these differences can not be accounted for using a simple spring model.

  20. Mundari as a flexible language

    DEFF Research Database (Denmark)

    Hengeveld, Kees; Rijkhoff, Jan

    2005-01-01

    In this paper we intend to show that Mundari is one of the languages without distinct classes of verbs and nouns as far as its basic, non-derived vocabulary is concerned. Our contribution is organized as follows. Section 2 briefly presents a typology of parts-of-speech (PoS) systems, followed by a...... critical evaluation of the three criteria Evans & Osada (E&O - this volume) use to establish the lack of word class distinctions in a language (section 3). In section 4 we present evidence to support our claim that Mundari has ‘flexible’ basic lexemes (i.e. there is no fundamental distinction between nouns...... and verbs), thus disputing E&O’s claim that Mundari has clearly definable classes of verbs and nouns. The last section is concerned with a set of grammatical features which correlate with the presence of flexible lexemes in a language. It is concluded that Mundari displays all of the predicted...

  1. Project Flexibility in Large Engineering Projects

    OpenAIRE

    Olsson, Nils

    2006-01-01

    Traditionally, projects tend to strive for increased predictability by managing details and attempting to bring all variables under control. However, experience shows that the chance of realising a plan without major amendments decreases with an increasing time horizon, which points to a need for flexible projects. A number of scholars argue that flexibility is necessary to face changes and uncertainty in the business environment. On the other hand, flexible projects are generally not describ...

  2. Performance and Usability of Flexible Membrane Keyboards

    OpenAIRE

    Shin, Dong-Jae

    2005-01-01

    Recently, many full-sized keyboards have been designed to fold in various ways in an attempt to make them more transportable. The flexible membrane keyboard, one type of full sized keyboard, is unique because it is made from silicon rubber, thus it is fully flexible and water resistant. Although a number of flexible keyboard characteristics are the same as standard keyboards (i.e. key size, shape and spacing), key-switch and key clicking mechanisms are inherently different. Since there is ...

  3. Flexibility in 21st Century Power Systems

    Energy Technology Data Exchange (ETDEWEB)

    Cochran, J.; Miller, M.; Zinaman, O.; Milligan, M.; Arent, D.; Palmintier, B.; O' Malley, M.; Mueller, S.; Lannoye, E.; Tuohy, A.; Kujala, B.; Sommer, M.; Holttinen, H.; Kiviluoma, J.; Soonee, S. K.

    2014-05-01

    Flexibility of operation--the ability of a power system to respond to change in demand and supply--is a characteristic of all power systems. Flexibility is especially prized in twenty-first century power systems, with higher levels of grid-connected variable renewable energy (primarily, wind and solar). This paper summarizes the analytic frameworks that have emerged to measure this characteristic and distills key principles of flexibility for policy makers.

  4. Flexible Working Time Arrangements in Bulgaria

    OpenAIRE

    Beleva, Iskra

    2009-01-01

    The objective of this paper is to analyze the flexible working time arrangements in Bulgaria, using a life-course perspective. Two important features have to be outlined, namely: underdeveloped flexible forms of employment in the country, including working time arrangement, and lack of previous analysis on flexible working time arrangements from the angle of life-course perspective. The author describes the regulatory framework, collective agreements at national and company level as a frame w...

  5. Holographic sensing for control of flexible structures

    OpenAIRE

    Barsky, Michael Frederick

    1990-01-01

    A state feedback control system for flexible structures implemented using a holographic sensor and optical processor is presented. Real-time holography provides a mechanism for sensing the distributed shape of a broad class of one and two-dimensional flexible structures in a form that can be processed using fixed optics. The optical processing solves the spillover problem in the theory of the control of flexible structures. The optical processing also simplifies the computation allowing the s...

  6. Does quality management drive labour flexibility?

    OpenAIRE

    Escrig Tena, Ana Belén; Bou Llusar, Juan Carlos; Roca Puig, Vicente; Beltrán Martín, Inmaculada

    2012-01-01

    This study aims to shed light on the contribution of quality management (QM) to labour flexibility by analysing the influence that QM has on internal and external labour flexibility. The study presents pioneer research in the empirical analysis of how QM impacts on labour flexibility. The hypotheses put forward are tested in an empirical study carried out on a sample of Spanish service companies. Structural equation modelling was used to analyse the data. The results indicate that QM is shown...

  7. Cash holdings, capital structure and financial flexibility

    OpenAIRE

    Chua, Shen Hwee

    2012-01-01

    This thesis is structured into two main parts to investigate the role of financial flexibility in firms’ liquidity and financing management. Financial flexibility is the most important practical determinant to managers when they make financing decisions. This missing link, financial flexibility, is identified by practitioners and used in this thesis to fill the missing gap between theory and practice in corporate finance. Part A of this thesis analyses the trends in firms’ internal financial ...

  8. Flexible depth of field photography.

    Science.gov (United States)

    Kuthirummal, Sujit; Nagahara, Hajime; Zhou, Changyin; Nayar, Shree K

    2011-01-01

    The range of scene depths that appear focused in an image is known as the depth of field (DOF). Conventional cameras are limited by a fundamental trade-off between depth of field and signal-to-noise ratio (SNR). For a dark scene, the aperture of the lens must be opened up to maintain SNR, which causes the DOF to reduce. Also, today's cameras have DOFs that correspond to a single slab that is perpendicular to the optical axis. In this paper, we present an imaging system that enables one to control the DOF in new and powerful ways. Our approach is to vary the position and/or orientation of the image detector during the integration time of a single photograph. Even when the detector motion is very small (tens of microns), a large range of scene depths (several meters) is captured, both in and out of focus. Our prototype camera uses a micro-actuator to translate the detector along the optical axis during image integration. Using this device, we demonstrate four applications of flexible DOF. First, we describe extended DOF where a large depth range is captured with a very wide aperture (low noise) but with nearly depth-independent defocus blur. Deconvolving a captured image with a single blur kernel gives an image with extended DOF and high SNR. Next, we show the capture of images with discontinuous DOFs. For instance, near and far objects can be imaged with sharpness, while objects in between are severely blurred. Third, we show that our camera can capture images with tilted DOFs (Scheimpflug imaging) without tilting the image detector. Finally, we demonstrate how our camera can be used to realize nonplanar DOFs. We believe flexible DOF imaging can open a new creative dimension in photography and lead to new capabilities in scientific imaging, vision, and graphics. PMID:21088319

  9. Supermarket Defrost Cycles As Flexible Reserve

    DEFF Research Database (Denmark)

    Pedersen, Rasmus; Sloth, Christoffer; Wisniewski, Rafal;

    2015-01-01

    This work analyses how supermarket defrost cycles can be used as flexible reserve in a smart grid context. The consumption flexibility originates from being able to shift defrost cycles in time, while adhering to the underlying refrigeration systems constraints. It is shown how this time constrai......This work analyses how supermarket defrost cycles can be used as flexible reserve in a smart grid context. The consumption flexibility originates from being able to shift defrost cycles in time, while adhering to the underlying refrigeration systems constraints. It is shown how this time...

  10. Material challenge for flexible organic devices

    Directory of Open Access Journals (Sweden)

    Jay Lewis

    2006-04-01

    Full Text Available Outside of the active device layers, there are a variety of requisite functional layers in flexible organic electronic devices. Whether the application is in displays, lighting, integrated circuits, or photovoltaics, there are materials challenges in implementing flexible and/or organic devices into practical applications. We highlight two topics that are common to most flexible electronic technologies. First, we describe the difficulty in developing suitable permeation barriers on polymer substrates, the approaches being taken to solve this problem, and their current status. Second, we highlight the limited mechanical ruggedness of brittle inorganic films and present approaches for improving overall device flexibility.

  11. Business Models for Power System Flexibility

    DEFF Research Database (Denmark)

    Boscan, Luis; Poudineh, Rahmatallah

    2016-01-01

    As intermittent, renewable resources gain more share in the generation mix, the need for power system flexibility increases more than ever. Parallel to this, technological change and the emergence of new players bringing about innovative solutions are boosting the development of flexibility......-enabling business models, adding new activities to the existing supply chain. Ranging from technological to market-based innovation, this chapter reviews the latest developments in these emerging models. The main conclusion is that when flexibility becomes scarce in the system, new players with their innovative...... business models will play an important role in ensuring sufficiency and efficiency of flexibility services....

  12. Distal histidine conformational flexibility in dehaloperoxidase from Amphitrite ornata

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Zuxu; de Serrano, Vesna; Betts, Laurie; Franzen, Stefan; (NCSU); (UNC)

    2009-01-28

    The enzyme dehaloperoxidase (DHP) from the terebellid polychaete Amphitrite ornata is a heme protein which has a globin fold but can function as both a hemoglobin and a peroxidase. As a peroxidase, DHP is capable of converting 2,4,6-trihalophenols to the corresponding 2,6-dihaloquinones in the presence of hydrogen peroxide. As a hemoglobin, DHP cycles between the oxy and deoxy states as it reversibly binds oxygen for storage. Here, it is reported that the distal histidine, His55, exhibits conformational flexibility in the deoxy form and is consequently observed in two solvent-exposed conformations more than 9.5 {angstrom} away from the heme. These conformations are analogous to the open conformation of sperm whale myoglobin. The heme iron in deoxy ferrous DHP is five-coordinate and has an out-of-plane displacement of 0.25 {angstrom} from the heme plane. The observation of five-coordinate heme iron with His55 in a remote solvent-exposed conformation is consistent with the hypothesis that His55 interacts with heme iron ligands through hydrogen bonding in the closed conformation. Since His55 is also displaced by the binding of 4-iodophenol in an internal pocket, these results provide new insight into the correlation between heme iron ligation, molecular binding in the distal pocket and the conformation of the distal histidine in DHP.

  13. Pressure dissociation of integration host factor–DNA complexes reveals flexibility-dependent structural variation at the protein–DNA interface

    OpenAIRE

    Senear, Donald F.; Tretyachenko-Ladokhina, Vira; Opel, Michael L.; Aeling, Kimberly A.; G Wesley Hatfield; Franklin, Laurie M.; Darlington, Reuben C.; Alexander Ross, J. B.

    2007-01-01

    E. coli Integration host factor (IHF) condenses the bacterial nucleoid by wrapping DNA. Previously, we showed that DNA flexibility compensates for structural characteristics of the four consensus recognition elements associated with specific binding (Aeling et al., J. Biol. Chem. 281, 39236–39248, 2006). If elements are missing, high-affinity binding occurs only if DNA deformation energy is low. In contrast, if all elements are present, net binding energy is unaffected by deformation energy. ...

  14. Stability and Sugar Recognition Ability of Ricin-Like Carbohydrate Binding Domains

    Energy Technology Data Exchange (ETDEWEB)

    Yao, Jianzhuang [ORNL; Nellas, Ricky B [ORNL; Glover, Mary M [ORNL; Shen, Tongye [ORNL

    2011-01-01

    Lectins are a class of proteins known for their novel binding to saccharides. Understanding this sugar recognition process can be crucial in creating structure-based designs of proteins with various biological roles. We focus on the sugar binding of a particular lectin, ricin, which has two -trefoil carbohydrate-binding domains (CRDs) found in several plant protein toxins. The binding ability of possible sites of ricin-like CRD has been puzzling. The apo and various (multiple) ligand-bound forms of the sugar-binding domains of ricin were studied by molecular dynamics simulations. By evaluating structural stability, hydrogen bond dynamics, flexibility, and binding energy, we obtained a detailed picture of the sugar recognition of the ricin-like CRD. Unlike what was previously believed, we found that the binding abilities of the two known sites are not independent of each other. The binding ability of one site is positively affected by the other site. While the mean positions of different binding scenarios are not altered significantly, the flexibility of the binding pockets visibly decreases upon multiple ligand binding. This change in flexibility seems to be the origin of the binding cooperativity. All the hydrogen bonds that are strong in the monoligand state are also strong in the double-ligand complex, although the stability is much higher in the latter form due to cooperativity. These strong hydrogen bonds in a monoligand state are deemed to be the essential hydrogen bonds. Furthermore, by examining the structural correlation matrix, the two domains are structurally one entity. Galactose hydroxyl groups, OH4 and OH3, are the most critical parts in both site 1 and site 2 recognition.

  15. Flexibility@Work 2013: yearly report on flexible labor and employment

    NARCIS (Netherlands)

    E. Berkhout; A. Heyma; J. Prins

    2013-01-01

    There is no clear evidence that the strong growth in the share of flexible labor relations between 2002 and 2007 points at a worldwide trend towards a larger share of flexible labor at the expense of traditional open-ended labor contracts. The growth in flexible labor varies too much between countri

  16. Magnetoelectric Nanocomposites for Flexible Electronics

    KAUST Repository

    Al-Nassar, Mohammed Y.

    2015-09-01

    Flexibility, low cost, versatility, miniaturization and multi-functionality are key aspects driving research and innovation in many branches of the electronics industry. With many anticipated emerging applications, like wearable, transparent and biocompatible devices, interest among the research community in pursuit for novel multifunctional miniaturized materials have been amplified. In this context, multiferroic polymer-based nanocomposites, possessing both ferroelectricity and ferromagnetism, are highly appealing. Most importantly, these nanocomposites possess tunable ferroelectric and ferromagnetic properties based on the parameters of their constituent materials as well as the magnetoelectric effect, which is the coupling between electric and magnetic properties. This tunability and interaction is a fascinating fundamental research field promising tremendous potential applications in sensors, actuators, data storage and energy harvesting. This dissertation work is devoted to the investigation of a new class of multiferroic polymer-based flexible nanocomposites, which exhibits excellent ferromagnetism and ferroelectricity simultaneously at room temperature, with the goal of understanding and optimizing the origin of their magnetoelectric coupling. The nanocomposites consist of high aspect ratio ferromagnetic nanowires (NWs) embedded inside a ferroelectric co-polymer, poly(vinylindene fluoride-trifluoroethylene), P(VDF-TrFE) matrix. First, electrochemical deposition of ferromagnetic NWs inside anodic aluminum oxide membranes is discussed. Characterization of electrodeposited iron, nickel and highly magnetostrictive iron-gallium alloy NWs was done using XRD, electron and magnetic force microscopy. Second, different nanocomposite films have been fabricated by means of spin coating and drop casting techniques. The effect of incorporation of NWs inside the ferroelectric polymer on its electroactive phase is discussed. The remanent and saturation polarization as well

  17. Cloud flexibility using DIRAC interware

    International Nuclear Information System (INIS)

    Communities of different locations are running their computing jobs on dedicated infrastructures without the need to worry about software, hardware or even the site where their programs are going to be executed. Nevertheless, this usually implies that they are restricted to use certain types or versions of an Operating System because either their software needs an definite version of a system library or a specific platform is required by the collaboration to which they belong. On this scenario, if a data center wants to service software to incompatible communities, it has to split its physical resources among those communities. This splitting will inevitably lead to an underuse of resources because the data centers are bound to have periods where one or more of its subclusters are idle. It is, in this situation, where Cloud Computing provides the flexibility and reduction in computational cost that data centers are searching for. This paper describes a set of realistic tests that we ran on one of such implementations. The test comprise software from three different HEP communities (Auger, LHCb and QCD phenomelogists) and the Parsec Benchmark Suite running on one or more of three Linux flavors (SL5, Ubuntu 10.04 and Fedora 13). The implemented infrastructure has, at the cloud level, CloudStack that manages the virtual machines (VM) and the hosts on which they run, and, at the user level, the DIRAC framework along with a VM extension that will submit, monitorize and keep track of the user jobs and also requests CloudStack to start or stop the necessary VM's. In this infrastructure, the community software is distributed via the CernVM-FS, which has been proven to be a reliable and scalable software distribution system. With the resulting infrastructure, users are allowed to send their jobs transparently to the Data Center. The main purpose of this system is the creation of flexible cluster, multiplatform with an scalable method for software distribution for

  18. Cloud flexibility using DIRAC interware

    Science.gov (United States)

    Fernandez Albor, Víctor; Seco Miguelez, Marcos; Fernandez Pena, Tomas; Mendez Muñoz, Victor; Saborido Silva, Juan Jose; Graciani Diaz, Ricardo

    2014-06-01

    Communities of different locations are running their computing jobs on dedicated infrastructures without the need to worry about software, hardware or even the site where their programs are going to be executed. Nevertheless, this usually implies that they are restricted to use certain types or versions of an Operating System because either their software needs an definite version of a system library or a specific platform is required by the collaboration to which they belong. On this scenario, if a data center wants to service software to incompatible communities, it has to split its physical resources among those communities. This splitting will inevitably lead to an underuse of resources because the data centers are bound to have periods where one or more of its subclusters are idle. It is, in this situation, where Cloud Computing provides the flexibility and reduction in computational cost that data centers are searching for. This paper describes a set of realistic tests that we ran on one of such implementations. The test comprise software from three different HEP communities (Auger, LHCb and QCD phenomelogists) and the Parsec Benchmark Suite running on one or more of three Linux flavors (SL5, Ubuntu 10.04 and Fedora 13). The implemented infrastructure has, at the cloud level, CloudStack that manages the virtual machines (VM) and the hosts on which they run, and, at the user level, the DIRAC framework along with a VM extension that will submit, monitorize and keep track of the user jobs and also requests CloudStack to start or stop the necessary VM's. In this infrastructure, the community software is distributed via the CernVM-FS, which has been proven to be a reliable and scalable software distribution system. With the resulting infrastructure, users are allowed to send their jobs transparently to the Data Center. The main purpose of this system is the creation of flexible cluster, multiplatform with an scalable method for software distribution for several

  19. Demand flexibility from residential heat pump

    DEFF Research Database (Denmark)

    Bhattarai, Bishnu Prasad; Bak-Jensen, Birgitte; Pillai, Jayakrishnan Radhakrishna;

    2014-01-01

    high thermal time constant, heat pumps (HP) can offer a great deal of flexibility in the future intelligent grids especially to compensate fluctuating generation. However, the HP flexibility is highly dependent on thermal demand profile, namely hot water and space heating demand. This paper proposes...

  20. Flexible carbon nanotubes electrode for neural recording.

    Science.gov (United States)

    Lin, Chia-Min; Lee, Yu-Tao; Yeh, Shih-Rung; Fang, Weileun

    2009-05-15

    This paper demonstrates a novel flexible carbon nanotubes (CNTs) electrode array for neural recording. In this device, the CNTs electrode arrays are partially embedded into the flexible Parylene-C film using a batch microfabrication process. Through this fabrication process, the CNTs can be exposed to increase the total sensing area of an electrode. Thus, the flexible CNTs electrode of low impedance is realized. In application, the flexible CNTs electrode has been employed to record the neural signal of a crayfish nerve cord for in vitro recording. The measurements demonstrate the superior performance of the presented flexible CNTs electrode with low impedance (11.07 kohms at 1 kHz) and high peak-to-peak amplitude action potential (about 410 microV). In addition, the signal-to-noise ratio (SNR) of the presented flexible CNTs electrode is about 257, whereas the SNR of the reference (a pair of Teflon-coated silver wires) is only 79. The simultaneous recording of the flexible CNTs electrode array is also demonstrated. Moreover, the flexible CNTs electrode has been employed to successfully record the spontaneous spikes from the crayfish nerve cord. The amplitude of the spontaneous peak-to-peak response is about 25 microV. PMID:19272765

  1. Flexible protective gloves: The emperor's new clothes

    International Nuclear Information System (INIS)

    The risk of developing skin cancer is estimated for interventional radiologists who do and do not wear thin, flexible protective leaded gloves. The use of these gloves is extremely expensive in terms of dollars per potential cancer prevented. Good radiographic practice without the use of flexible protective gloves provides adequate protection

  2. CHANGE MANAGEMENT ESSENTIALS TO MANAGE BUSINESS FLEXIBILITY

    OpenAIRE

    Cãtãlin Cristian BABALÂC; Mihaela UDÃ

    2014-01-01

    The present paper has as objective to introduce essential concepts that stand as basis for change and risk management, having a direct influence on flexibility. The competitive advantage represented by the company flexibility either if it is achieved by technology or with the help of human factor follows a path characterized by risk and possible resistance to what is the normal state.

  3. Modelling Flexible Pavement Response and Performance

    DEFF Research Database (Denmark)

    Ullidtz, Per

    This textbook is primarily concerned with models for predicting the future condition of flexible pavements, as a function of traffic loading, climate, materials, etc., using analytical-empirical methods.......This textbook is primarily concerned with models for predicting the future condition of flexible pavements, as a function of traffic loading, climate, materials, etc., using analytical-empirical methods....

  4. Semiconductor films on flexible iridium substrates

    Science.gov (United States)

    Goyal, Amit

    2005-03-29

    A laminate semiconductor article includes a flexible substrate, an optional biaxially textured oxide buffer system on the flexible substrate, a biaxially textured Ir-based buffer layer on the substrate or the buffer system, and an epitaxial layer of a semiconductor. Ir can serve as a substrate with an epitaxial layer of a semiconductor thereon.

  5. Studies of a flexible heliac configuration

    International Nuclear Information System (INIS)

    This paper documents a detailed study of the Flexible Heliac configuration. The remarkable flexibility of this device - which allows variation of the rotational transform, shear, and magnetic well depth over a relatively wide range - is described. Engineering considerations of error fields, finite cross-section conductors, and plasma coil clearances are also discussed

  6. "Flexibility", Community and Making Parents Responsible

    Science.gov (United States)

    McGowan, Wayne S.

    2005-01-01

    This article draws on Foucault's concept of governmentality to explore how recent political moves to legalise "flexibility" mobilises education authorities to make "community" a technical means of achieving the political objective of schooling the child. I argue that "flexibility" in this sense is a neo-liberal strategy that shifts relations…

  7. SHBG (Sex Hormone Binding Globulin)

    Science.gov (United States)

    ... as: Testosterone-estrogen Binding Globulin; TeBG Formal name: Sex Hormone Binding Globulin Related tests: Testosterone , Free Testosterone, ... I should know? How is it used? The sex hormone binding globulin (SHBG) test may be used ...

  8. IEA EBC Annex 67 Energy Flexible Buildings

    DEFF Research Database (Denmark)

    Marszal, Anna Joanna; Jensen, Søren Østergaard

    2016-01-01

    know ledge on and demonstration of the Energy Flexibility Buildings can provide for the energy grids as well of to identify critical aspects and possible solutions to manage this Energy Flexibility. The paper discusses the background, the aims and the work plan of IEA (International Energy Agency) EBC......The foreseen large deployment of renewable energy sources may seriously affect the stability of energy grids. It will be necessary to control energy consumption to match instantaneous energy production. The built-in Energy Flexibility in buildings may be utilized for stabilizing the energy grids......, allowing for a larger roll out of renewable technologies. The Energy Flexibility of a building is the ability to manage its energy demand and generation according to local climate conditions, user needs and grid requirements. Energy Flexibility of buildings will thus allow for demand side management and...

  9. Smart Tendon Actuated Flexible Actuator

    Directory of Open Access Journals (Sweden)

    Md. Masum Billah

    2015-01-01

    Full Text Available We investigate the kinematic feasibility of a tendon-based flexible parallel platform actuator. Much of the research on tendon-driven Stewart platforms is devoted either to the completely restrained positioning mechanism (CRPM or to one particular type of the incompletely restrained positioning mechanism (IRPM where the external force is provided by the gravitational pull on the platform such as in cable-suspended Stewart platforms. An IRPM-based platform is proposed which uses the external force provided by a compliant member. The compliant central column allows the configuration to achieve n DOFs with n tendons. In particular, this investigation focuses on the angular deflection of the upper platform with respect to the lower platform. The application here is aimed at developing a linkable module that can be connected to one another so as to form a “snake robot” of sorts. Since locomotion takes precedence over positioning in this application, a 3-DOF Stewart platform is adopted. For an arbitrary angular displace of the end-effector, the corresponding length of each tendon can be determined through inverse kinematics. Mathematical singularities are investigated using the traditional analytical method of defining the Jacobian.

  10. Mechanical flexible joint design document

    Science.gov (United States)

    Daily, Vic

    1993-01-01

    The purpose of this report is to document the status of the Mechanical Flexible Joint (MFJ) Design Subtask with the intent of halting work on the design. Recommendations for future work is included in the case that the task is to be resumed. The MFJ is designed to eliminate two failure points from the current flex joint configuration, the inner 'tripod configuration' and the outer containment jacket. The MFJ will also be designed to flex 13.5 degrees and have three degrees of freedom. By having three degrees of freedom, the MFJ will allow the Low Pressure Fuel Duct to twist and remove the necessity to angulate the full 11 degrees currently required. The current flex joints are very labor intensive and very costly and a simple alternative is being sought. The MFJ is designed with a greater angular displacement, with three degrees of freedom, to reside in the same overall envelope, to meet weight constraints of the current bellows, to be compatible with cryogenic fuel and oxidizers, and also to be man-rated.

  11. Structural Basis for Binding of RNA and Cofactor by a KsgA Methyltransferase

    Energy Technology Data Exchange (ETDEWEB)

    Tu, Chao; Tropea, Joseph E.; Austin, Brian P.; Court, Donald L.; Waugh, David S.; Ji, Xinhua; (NCI)

    2009-03-27

    Among methyltransferases, KsgA and the reaction it catalyzes are conserved throughout evolution. However, the specifics of substrate recognition by the enzyme remain unknown. Here we report structures of Aquifex aeolicus KsgA, in its ligand-free form, in complex with RNA, and in complex with both RNA and S-adenosylhomocysteine (SAH, reaction product of cofactor S-adenosylmethionine), revealing critical structural information on KsgA-RNA and KsgA-SAH interactions. Moreover, the structures show how conformational changes that occur upon RNA binding create the cofactor-binding site. There are nine conserved functional motifs (motifs IVIII and X) in KsgA. Prior to RNA binding, motifs I and VIII are flexible, each exhibiting two distinct conformations. Upon RNA binding, the two motifs become stabilized in one of these conformations, which is compatible with the binding of SAH. Motif X, which is also stabilized upon RNA binding, is directly involved in the binding of SAH.

  12. Evolved streptavidin mutants reveal key role of loop residue in high-affinity binding

    OpenAIRE

    Magalhães, Maria L. B.; Czekster, Clarissa Melo; Guan, Rong; Malashkevich, Vladimir N.; Almo, Steven C.; Levy, Matthew

    2011-01-01

    We have performed a detailed analysis of streptavidin variants with altered specificity towards desthiobiotin. In addition to changes in key residues which widen the ligand binding pocket and accommodate the more structurally flexible desthiobiotin, the data revealed the role of a key, non-active site mutation at the base of the flexible loop (S52G) which slows dissociation of this ligand by approximately sevenfold. Our data suggest that this mutation results in the loss of a stabilizing cont...

  13. Consistent improvement of cross docking results using binding site ensembles generated with Elastic Network Normal Modes

    OpenAIRE

    Rueda, Manuel; Bottegoni, Giovanni; Abagyan, Ruben

    2009-01-01

    The representation of protein flexibility is still a challenge for the state-of-the-art flexible ligand docking protocols. In this article we use a large and diverse benchmark to prove that is possible to improve consistently the cross docking performance against a single receptor conformation by using an equilibrium ensemble of binding site conformers. The benchmark contained 28 proteins, and the top ranked near native poses for the ligand were found 20% more efficiently than using a single ...

  14. Surface-grafted polymer-assisted electroless deposition of metals for flexible and stretchable electronics.

    Science.gov (United States)

    Liu, Xuqing; Zhou, Xuechang; Li, Yi; Zheng, Zijian

    2012-05-01

    Surface-grafted polymers, that is, ultrathin layers of polymer coating covalently tethered to a surface, can serve as a particularly promising nanoplatform for electroless deposition (ELD) of metal thin films and patterned structures. Such polymers consist of a large number of well-defined binding sites for highly efficient and selective uptake of ELD catalysts. Moreover, the polymer chains provide flexible 3D network structures to trap the electrolessly deposited metal particles, leading to strong metal-substrate adhesion. In the past decade, surface-grafted polymers have been demonstrated as efficient nanoplatforms for fabricating durable and high-performance metal coatings by ELD on plastic substrates for applications in flexible and stretchable electronics. This focus review summarizes these recent advances, with a particular focus on applications in polymeric flexible and stretchable substrates. An outlook on the future challenges and opportunities in this field is given at the end of this paper. PMID:22392811

  15. Exploiting protein flexibility to predict the location of allosteric sites

    Directory of Open Access Journals (Sweden)

    Panjkovich Alejandro

    2012-10-01

    Full Text Available Abstract Background Allostery is one of the most powerful and common ways of regulation of protein activity. However, for most allosteric proteins identified to date the mechanistic details of allosteric modulation are not yet well understood. Uncovering common mechanistic patterns underlying allostery would allow not only a better academic understanding of the phenomena, but it would also streamline the design of novel therapeutic solutions. This relatively unexplored therapeutic potential and the putative advantages of allosteric drugs over classical active-site inhibitors fuel the attention allosteric-drug research is receiving at present. A first step to harness the regulatory potential and versatility of allosteric sites, in the context of drug-discovery and design, would be to detect or predict their presence and location. In this article, we describe a simple computational approach, based on the effect allosteric ligands exert on protein flexibility upon binding, to predict the existence and position of allosteric sites on a given protein structure. Results By querying the literature and a recently available database of allosteric sites, we gathered 213 allosteric proteins with structural information that we further filtered into a non-redundant set of 91 proteins. We performed normal-mode analysis and observed significant changes in protein flexibility upon allosteric-ligand binding in 70% of the cases. These results agree with the current view that allosteric mechanisms are in many cases governed by changes in protein dynamics caused by ligand binding. Furthermore, we implemented an approach that achieves 65% positive predictive value in identifying allosteric sites within the set of predicted cavities of a protein (stricter parameters set, 0.22 sensitivity, by combining the current analysis on dynamics with previous results on structural conservation of allosteric sites. We also analyzed four biological examples in detail, revealing

  16. Flexible segments modulate co-folding of dUTPase and nucleocapsid proteins

    OpenAIRE

    Nemeth-Pongracz, V.; Barabas, O.; Fuxreiter, M; Simon, I; Pichova, I.; Rumlova, M.; Zabranska, H.; Svergun, D. I.; Petoukhov, M.; Harmat, V.; Klement, E.; Hunyadi-Gulyas, E.; Medzihradszky, K. F.; Konya, E.; Vertessy, B G

    2006-01-01

    The homotrimeric fusion protein nucleocapsid (NC)-dUTPase combines domains that participate in RNA/DNA folding, reverse transcription, and DNA repair in Mason-Pfizer monkey betaretrovirus infected cells. The structural organization of the fusion protein remained obscured by the N- and C-terminal flexible segments of dUTPase and the linker region connecting the two domains that are invisible in electron density maps. Small-angle X-ray scattering reveals that upon oligonucleotide binding the NC...

  17. An assessment of the EU proposal for ceilings on the use of Kyoto flexibility mechanisms

    OpenAIRE

    Zhang, ZhongXiang

    2000-01-01

    The Kyoto Protocol is the first international environmental agreement that sets legally binding greenhouse gas emissions targets and timetables for Annex I countries. It incorporates emissions trading, joint implementation and the clean development mechanism. Because each of the Articles defining the three flexibility mechanisms carries wording that the use of the mechanism must be supplemental to domestic actions, the supplementarity provisions have been the focus of the international climat...

  18. Probabilistic Model to Treat Flexibility in Molecular Contacts

    CERN Document Server

    Hakulinen, Riku

    2016-01-01

    Evaluating accessible conformational space is computationally expensive and thermal motions are partly neglected in computer models of molecular interactions. This produces error into the estimates of binding strength. We introduce a method for modelling interactions so that structural flexibility is inherently taken into account. It has a statistical model for 3D properties of 'nonlocal' contacts and a physics based description of 'local' interactions, based on mechanical torque. The form of the torque barrier is derived using a representation of the local electronic structure, which is presumed to improve transferability, compared to traditional force fields. The nonlocal contacts are more distant than 1-4 interactions and Target-atoms are represented by 3D probability densities. Probability mass quantifies strength of contact and is calculated as an overlap integral. Repulsion is described by negative probability density, allowing probability mass to be used as the descriptor of contact preference. As a re...

  19. Fast antibody fragment motion: flexible linkers act as entropic spring.

    Science.gov (United States)

    Stingaciu, Laura R; Ivanova, Oxana; Ohl, Michael; Biehl, Ralf; Richter, Dieter

    2016-01-01

    A flexible linker region between three fragments allows antibodies to adjust their binding sites to an antigen or receptor. Using Neutron Spin Echo Spectroscopy we observed fragment motion on a timescale of 7 ns with motional amplitudes of about 1 nm relative to each other. The mechanistic complexity of the linker region can be described by a spring model with Brownian motion of the fragments in a harmonic potential. Displacements, timescale, friction and force constant of the underlying dynamics are accessed. The force constant exhibits a similar strength to an entropic spring, with friction of the fragment matching the unbound state. The observed fast motions are fluctuations in pre-existing equilibrium configurations. The Brownian motion of domains in a harmonic potential is the appropriate model to examine functional hinge motions dependent on the structural topology and highlights the role of internal forces and friction to function. PMID:27020739

  20. Single molecule binding dynamics measured with atomic force microscopy

    International Nuclear Information System (INIS)

    We present a new method to analyse simultaneous Topography and RECognition Atomic Force Microscopy data such that it becomes possible to measure single molecule binding rates of surface bound proteins. We have validated this method on a model system comprising a S-layer surface modified with Strep-tagII for binding sites and strep-tactin bound to an Atomic Force Microscope tip through a flexible Poly-Ethylene-Glycol linker. At larger distances, the binding rate is limited by the linker, which limits the diffusion of the strep-tactin molecule, but at lateral distances below 3 nm, the binding rate is solely determined by the intrinsic molecular characteristics and the surface geometry and chemistry of the system. In this regime, Kon as determined from single molecule TREC data is in agreement with Kon determined using traditional biochemical methods. - Highlights: • We discuss the importance of studying single molecule binding rates for surface bound proteins. • We show measurements of single molecule binding rates on a model system using AFM. • We discuss the influence of various components on the measured binding rates

  1. The balance of flexibility and rigidity in the active site residues of hen egg white lysozyme

    Institute of Scientific and Technical Information of China (English)

    Qi Jian-Xun; Jiang Fan

    2011-01-01

    The crystallographic temperature factors (B factor) of individual atoms contain important information about the thermal motion of the atoms in a macromolecule. Previously the theory of flexibility of active site has been established based on the observation that the enzyme activity is sensitive to low concentration denaturing agents. It has been found that the loss of enzyme activity occurs well before the disruption of the three-dimensional structural scaffold of the enzyme. To test the theory of conformational flexibility of enzyme active site, crystal structures were perturbed by soaking in low concentration guanidine hydrochloride solutions. It was found that many lysozyme crystals tested could still diffract until the concentration of guanidine hydrochloride reached 3 M. It was also found that the B factors averaged over individually collected data sets were more accurate. Thus it suggested that accurate measurement of crystal temperature factors could be achieved for medium-high or even medium resolution crystals by averaging over multiple data sets. Furthermore, we found that the correctly predicted active sites included not only the more flexible residues, but also some more rigid residues. Both the flexible and the rigid residues in the active site played an important role in forming the active site residue network, covering the majority of the substrate binding residues. Therefore, this experimental prediction method may be useful for characterizing the binding site and the function of a protein, such as drug targeting.

  2. Flexible time domain averaging technique

    Science.gov (United States)

    Zhao, Ming; Lin, Jing; Lei, Yaguo; Wang, Xiufeng

    2013-09-01

    Time domain averaging(TDA) is essentially a comb filter, it cannot extract the specified harmonics which may be caused by some faults, such as gear eccentric. Meanwhile, TDA always suffers from period cutting error(PCE) to different extent. Several improved TDA methods have been proposed, however they cannot completely eliminate the waveform reconstruction error caused by PCE. In order to overcome the shortcomings of conventional methods, a flexible time domain averaging(FTDA) technique is established, which adapts to the analyzed signal through adjusting each harmonic of the comb filter. In this technique, the explicit form of FTDA is first constructed by frequency domain sampling. Subsequently, chirp Z-transform(CZT) is employed in the algorithm of FTDA, which can improve the calculating efficiency significantly. Since the signal is reconstructed in the continuous time domain, there is no PCE in the FTDA. To validate the effectiveness of FTDA in the signal de-noising, interpolation and harmonic reconstruction, a simulated multi-components periodic signal that corrupted by noise is processed by FTDA. The simulation results show that the FTDA is capable of recovering the periodic components from the background noise effectively. Moreover, it can improve the signal-to-noise ratio by 7.9 dB compared with conventional ones. Experiments are also carried out on gearbox test rigs with chipped tooth and eccentricity gear, respectively. It is shown that the FTDA can identify the direction and severity of the eccentricity gear, and further enhances the amplitudes of impulses by 35%. The proposed technique not only solves the problem of PCE, but also provides a useful tool for the fault symptom extraction of rotating machinery.

  3. Conserved high-affinity NF-kappa B binding site in the interferon regulatory factor-1 promoter is not occupied by NF-kappa B in vivo and is transcriptionally inactive.

    Science.gov (United States)

    Rein, T; Schreck, R; Willenbrink, W; Neubert, W J; Zorbas, H; Bäuerle, P A

    1995-01-01

    The promoter of the interferon regulatory factor-1 (IRF-1) gene contains at position -47 to -38 an evolutionary conserved binding sequence for the inducible transcription factor NF-kappa B. This site is highly homologous to a transcriptionally active site from the MHC class I enhancer. In this study, we show by in vitro assays using purified NF-kappa B that the kappa B motif in the IRF-1 promoter binds the factor specifically and with high affinity, comparable to various other cis-acting kappa B elements. Two copies of the IRF-1 kappa B site fused to the heterologous c-fos promoter conferred induction of a chloramphenicol acetyl transferase (CAT) reported gene in response to stimulation of L929 fibroblasts with various NF-kappa B inducers, such as tumor necrosis factor alpha (TNF alpha) or phorbol 12-myristate 13-acetate (PMA). Mutation of the binding site completely abolished transcriptional inducibility of the heterologous promoter. Surprisingly, the same IRF-1 kappa B motif in context of the homologous IRF-1 promoter was transcriptionally inactive in CAT assays. The very weak induction of the IRF-1 promoter in response to TNF treatment or infection of fibroblasts with Newcastle disease virus (NDV) was barely affected by point mutation of the kappa B site or loss of the site by truncation of the promoter. Analysis of the occupational state of the chromosomal IRF-1 kappa B site by in vivo foot-printing revealed that no footprint was induced over the kappa B motif in the IRF-1 promoter after PMA treatment of L929 fibroblast cells, despite the simultaneous induction of IRF-1 mRNA and NF-kappa B binding activity. Constitutive footprints were detected at a CCAAT and GC-rich region in the promoter. This is the first example of a high-affinity NF-kappa B binding site within a promoter which may not participate in transcriptional regulation under conditions activating NF-kappa B DNA binding and gene expression. PMID:8867671

  4. Feasibility and flexibility for a trigeneration system

    Energy Technology Data Exchange (ETDEWEB)

    Lai, Sau Man; Hui, Chi Wai [Department of Chemical and Biomolecular Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR (China)

    2009-10-15

    Trigeneration system, which produces heat, cold and electricity simultaneously, is generally designed based on the nominal condition. However, the utility demands are seldom fixed and they are usually changing in periodical manner with the climate and the human activities. These demand changes make the system design difficult. To ensure operability, the system should be feasible and flexible to tackle such demand variations. Over-sizing, thermal storage and flexibility re-allocation can be used to improve a trigeneration system's feasibility and flexibility. These techniques may enlarge the feasible operating region, change and shift the expected utility production demands and interchange between different generation capabilities according to the demand requirements. As a result, the process feasibility and flexibility can be improved. With feasible operation ensured, process flexibility can be considered under economic trade-offs. A flexible design with reasonable investment and operating costs provides additional benefits to cater demand changes in the future. In this study, process flexibility and feasibility characteristics are proposed and evaluated from a new perspective. These are demonstrated in the trigeneration system design with a pre-defined structure to handle periodical utility demand deviations in a commercial building complex. (author)

  5. Flexible, transparent, and conductive defrosting glass

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Jingjing [Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742 (United States); Fang, Zhiqiang; Zhu, Hongli; Gao, Binyu [Department of Materials Science and Engineering, University of Maryland, College Park, MD 20742 (United States); Garner, Sean; Cimo, Pat [Corning Incorporated, Corning, NY 14831 (United States); Barcikowski, Zachary [Department of Materials Science and Engineering, University of Maryland, College Park, MD 20742 (United States); Mignerey, Alice [Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742 (United States); Hu, Liangbing, E-mail: binghu@umd.edu [Department of Materials Science and Engineering, University of Maryland, College Park, MD 20742 (United States)

    2014-04-01

    Flexible and transparent electronics play a predominant role in the next-generation electrical devices. In this study, a printable aqueous graphene oxide (GO) ink that enables direct deposition of GO onto flexible glass substrates is demonstrated and its application on fabricating a transparent, conductive, and flexible glass device by solution coating process is investigated as well. A uniform GO layer is formed on the flexible glass through Meyer-rod coating followed by an annealing process to reduce GO into graphene. The obtained thermally reduced graphene oxide (RGO) flexible glass has a transmittance of over 40%, as well as a sheet resistance of ∼ 5 × 10{sup 3} Ω/sq. In addition, a defrosting window fabricated from the RGO coated flexible glass is demonstrated, which shows excellent defrosting performance. - Highlights: • A facile synthesis of aqueous graphene oxide (GO) suspension is demonstrated. • Scalable printing of GO suspension is achieved with Meyer-rod coating technique. • A flexible glass is utilized as a substrate for the deposition of GO suspension. • Reduced graphene oxide films show improved conductivity with great transmittance. • Its potential to be applied in window defrosting is demonstrated and illustrated.

  6. Flexible, transparent, and conductive defrosting glass

    International Nuclear Information System (INIS)

    Flexible and transparent electronics play a predominant role in the next-generation electrical devices. In this study, a printable aqueous graphene oxide (GO) ink that enables direct deposition of GO onto flexible glass substrates is demonstrated and its application on fabricating a transparent, conductive, and flexible glass device by solution coating process is investigated as well. A uniform GO layer is formed on the flexible glass through Meyer-rod coating followed by an annealing process to reduce GO into graphene. The obtained thermally reduced graphene oxide (RGO) flexible glass has a transmittance of over 40%, as well as a sheet resistance of ∼ 5 × 103 Ω/sq. In addition, a defrosting window fabricated from the RGO coated flexible glass is demonstrated, which shows excellent defrosting performance. - Highlights: • A facile synthesis of aqueous graphene oxide (GO) suspension is demonstrated. • Scalable printing of GO suspension is achieved with Meyer-rod coating technique. • A flexible glass is utilized as a substrate for the deposition of GO suspension. • Reduced graphene oxide films show improved conductivity with great transmittance. • Its potential to be applied in window defrosting is demonstrated and illustrated

  7. ESF [Exploratory Shaft Facility] flexibility analysis

    International Nuclear Information System (INIS)

    This report directs that uncertainty allowances be included within the ESF facilities. The recommendations herein developed are intended as input to Title II Design criteria. Flexibility is measured first by lineal ft of drift, and then by hoisting rate and capacity of supporting utilities and services. A defined probability of need shows an extra 10,000 ft of drift for the first level of flexibility responding to testing and operations, and over 60,000 ft of drift for the second level of flexibility which recognizes possible need for perimeter drifting to investigate geologic stratigraphy. Observing there will be time constraints, a single shaft muck hoisting rate up to 170 to 250 tons per hour is recommended. The potential hoisting rate recommended for flexibility should be satisfied by a hoist approximately equivalent to, or conveniently upgraded from those being considered for sinking and construction, or 1000 horsepower. The cost of flexibility is limited to engineering planning and design (mostly conceptual) which makes later expansion achievable, and to selected items for initial construction where later upgrading would be impractical, impossible, or very costly. The cost is fixed to the level of flexibility and does not vary with excavated footage. The incremental margin is only a small fraction of the additional footage made available. Flexibility presents a strategy and not a position of design or technology. Examples used in this report are intended to be illustrative only, and not to lead design or cost estimates. 7 tabs

  8. Flexible Learning in an Information Society

    Directory of Open Access Journals (Sweden)

    Edited by Badrul H. Khan and Reviewed by Dr. Adile Aşkım Kurt

    2007-01-01

    Full Text Available This book was edited by, Badrul H. Khan, Director of the Educational Technology Leadership graduate cohort program at The George Washington University, and published by Information Science Publishing an imprint of Idea Group Inc.The information age has brought about many ways for educators to help their students learn, creating an atmosphere where the learners desire flexibility in the way they learn. With the increasing use of variety of approaches for learning, students’ preferences are changing from wanting to be taught mostly in lectures or direct training sessions to wanting increased flexibility. Students expect on demand anytime/anywhere high-quality learning environments with good support services. They want increased flexibility.This book uses a flexible learning framework to create meaningful flexible learning environments. This framework consists of eight factors; institutional, management, technological, pedagogical, ethical, interface design, resource support, and evaluation. This book presents a broad understanding of the emerging field of flexible learning and provides guidance in creating these environments. The book has thirty chapters, encompass various critical issues dealing with one or more categories of the flexible learning framework and offer a variety of points of view on these issues.

  9. Cholesterol-binding viral proteins in virus entry and morphogenesis.

    Science.gov (United States)

    Schroeder, Cornelia

    2010-01-01

    Up to now less than a handful of viral cholesterol-binding proteins have been characterized, in HIV, influenza virus and Semliki Forest virus. These are proteins with roles in virus entry or morphogenesis. In the case of the HIV fusion protein gp41 cholesterol binding is attributed to a cholesterol recognition consensus (CRAC) motif in a flexible domain of the ectodomain preceding the trans-membrane segment. This specific CRAC sequence mediates gp41 binding to a cholesterol affinity column. Mutations in this motif arrest virus fusion at the hemifusion stage and modify the ability of the isolated CRAC peptide to induce segregation of cholesterol in artificial membranes.Influenza A virus M2 protein co-purifies with cholesterol. Its proton translocation activity, responsible for virus uncoating, is not cholesterol-dependent, and the transmembrane channel appears too short for integral raft insertion. Cholesterol binding may be mediated by CRAC motifs in the flexible post-TM domain, which harbours three determinants of binding to membrane rafts. Mutation of the CRAC motif of the WSN strain attenuates virulence for mice. Its affinity to the raft-non-raft interface is predicted to target M2 protein to the periphery of lipid raft microdomains, the sites of virus assembly. Its influence on the morphology of budding virus implicates M2 as factor in virus fission at the raft boundary. Moreover, M2 is an essential factor in sorting the segmented genome into virus particles, indicating that M2 also has a role in priming the outgrowth of virus buds.SFV E1 protein is the first viral type-II fusion protein demonstrated to directly bind cholesterol when the fusion peptide loop locks into the target membrane. Cholesterol binding is modulated by another, proximal loop, which is also important during virus budding and as a host range determinant, as shown by mutational studies. PMID:20213541

  10. Flexible Molybdenum Electrodes towards Designing Affinity Based Protein Biosensors.

    Science.gov (United States)

    Kamakoti, Vikramshankar; Panneer Selvam, Anjan; Radha Shanmugam, Nandhinee; Muthukumar, Sriram; Prasad, Shalini

    2016-01-01

    Molybdenum electrode based flexible biosensor on porous polyamide substrates has been fabricated and tested for its functionality as a protein affinity based biosensor. The biosensor performance was evaluated using a key cardiac biomarker; cardiac Troponin-I (cTnI). Molybdenum is a transition metal and demonstrates electrochemical behavior upon interaction with an electrolyte. We have leveraged this property of molybdenum for designing an affinity based biosensor using electrochemical impedance spectroscopy. We have evaluated the feasibility of detection of cTnI in phosphate-buffered saline (PBS) and human serum (HS) by measuring impedance changes over a frequency window from 100 mHz to 1 MHz. Increasing changes to the measured impedance was correlated to the increased dose of cTnI molecules binding to the cTnI antibody functionalized molybdenum surface. We achieved cTnI detection limit of 10 pg/mL in PBS and 1 ng/mL in HS medium. The use of flexible substrates for designing the biosensor demonstrates promise for integration with a large-scale batch manufacturing process. PMID:27438863

  11. Computational study of ligand binding in lipid transfer proteins: Structures, interfaces, and free energies of protein-lipid complexes

    OpenAIRE

    Fernandez Pacios, Luis; Gomez Casado, Cristina; Tordesillas Villuendas, Leticia; Palacín Gómez, Aranzazu; Sanchez-Monge Laguna De Rins, Maria Rosa; Díaz Perales, Araceli

    2012-01-01

    Plant nonspecific lipid transfer proteins (nsLTPs) bind a wide variety of lipids, which allows them to perform disparate functions. Recent reports on their multifunctionality in plant growth processes have posed new questions on the versatile binding abilities of these proteins. The lack of binding specificity has been customarily explained in qualitative terms on the basis of a supposed structural flexibility and nonspecificity of hydrophobic protein-ligand interactions. We present here a co...

  12. Anomalous dispersion analysis of inhibitor flexibility: a case study of the kinase inhibitor H-89

    International Nuclear Information System (INIS)

    The anomalous dispersion signal of the bromine-containing kinase inhibitor H-89 was used to characterize discrete binding modes of the compound when complexed with the catalytic subunit of protein kinase A. With its ability to show the interactions between drug-target proteins and small-molecule ligands, X-ray crystallography is an essential tool in drug-discovery programmes. However, its usefulness can be limited by crystallization artifacts or by the data resolution, and in particular when assumptions of unimodal binding (and isotropic motion) do not apply. Discrepancies between the modelled crystal structure and the physiological range of structures generally prevent quantitative estimation of binding energies. Improved crystal structure resolution will often not aid energy estimation because the conditions which provide the highest rigidity and resolution are not likely to reflect physiological conditions. Instead, strategies must be employed to measure and model flexibility and multiple binding modes to supplement crystallographic information. One useful tool is the use of anomalous dispersion for small molecules that contain suitable atoms. Here, an analysis of the binding of the kinase inhibitor H-89 to protein kinase A (PKA) is presented. H-89 contains a bromobenzene moiety that apparently binds with multiple conformations in the kinase ATP pocket. Using anomalous dispersion methods, it was possible to resolve these conformations into two distinct binding geometries

  13. Rigidity and flexibility of biological networks

    CERN Document Server

    Gaspar, Merse E

    2012-01-01

    The network approach became a widely used tool to understand the behaviour of complex systems in the last decade. We start from a short description of structural rigidity theory. A detailed account on the combinatorial rigidity analysis of protein structures, as well as local flexibility measures of proteins and their applications in explaining allostery and thermostability is given. We also briefly discuss the network aspects of cytoskeletal tensegrity. Finally, we show the importance of the balance between functional flexibility and rigidity in protein-protein interaction, metabolic, gene regulatory and neuronal networks. Our summary raises the possibility that the concepts of flexibility and rigidity can be generalized to all networks.

  14. Rigidity and flexibility of biological networks.

    Science.gov (United States)

    Gáspár, Merse E; Csermely, Peter

    2012-11-01

    The network approach became a widely used tool to understand the behaviour of complex systems in the last decade. We start from a short description of structural rigidity theory. A detailed account on the combinatorial rigidity analysis of protein structures, as well as local flexibility measures of proteins and their applications in explaining allostery and thermostability is given. We also briefly discuss the network aspects of cytoskeletal tensegrity. Finally, we show the importance of the balance between functional flexibility and rigidity in protein-protein interaction, metabolic, gene regulatory and neuronal networks. Our summary raises the possibility that the concepts of flexibility and rigidity can be generalized to all networks. PMID:23165349

  15. Ultrabarrier Flexible Substrates for Flat Panel Displays

    Energy Technology Data Exchange (ETDEWEB)

    Burrows, Paul E.; Graff, Gordon L.; Gross, Mark E.; Martin, Peter M.; Shi, Ming-Kun; Hall, Michael G.; Mast, Eric S.; Bonham, Charles C.; Bennett, Wendy D.; Sullivan, Michael B.

    2001-05-01

    We describe a flexible, transparent plastic substrate for flat panel display applications. Using roll-coating techniques, we apply a composite thin film barrier to commercially available polymers, which restricts moisture and oxygen permeation to undetectable levels. The barrier film can be capped with a thin film of transparent conductive oxide in the same roll-coater, yielding an engineered substrate (Barix™) for next generation, rugged, lightweight or flexible displays. The substrate is sufficiently impermeable to moisture and oxygen for application to moisture-sensitive display applications, such as organic light emitting displays (OLEDs). This enables, for the first time, lightweight and flexible emissive organic displays.

  16. Flexible structured high-frequency film bulk acoustic resonator for flexible wireless electronics

    International Nuclear Information System (INIS)

    Flexible electronics have inspired many novel and very important applications in recent years and various flexible electronic devices such as diodes, transistors, circuits, sensors, and radiofrequency (RF) passive devices including antennas and inductors have been reported. However, the lack of a high-performance RF resonator is one of the key bottlenecks to implement flexible wireless electronics. In this study, for the first time, a novel ultra-flexible structured film bulk acoustic resonator (FBAR) is proposed. The flexible FBAR is fabricated on a flexible polyimide substrate using piezoelectric thin film aluminum nitride (AlN) for acoustic wave excitation. Both the shear wave and longitudinal wave can be excited under the surface interdigital electrodes configuration we proposed. In the case of the thickness extension mode, a flexible resonator with a working frequency as high as of 5.2325 GHz has been realized. The resonators stay fully functional under bending status and after repeated bending and re-flattening operations. This flexible high-frequency resonator will serve as a key building block for the future flexible wireless electronics, greatly expanding the application scope of flexible electronics. (paper)

  17. Electrostatics and Intrinsic Disorder Drive Translocon Binding of the SRP Receptor FtsY.

    Science.gov (United States)

    Lakomek, Nils-Alexander; Draycheva, Albena; Bornemann, Thomas; Wintermeyer, Wolfgang

    2016-08-01

    Integral membrane proteins in bacteria are co-translationally targeted to the SecYEG translocon for membrane insertion via the signal recognition particle (SRP) pathway. The SRP receptor FtsY and its N-terminal A domain, which is lacking in any structural model of FtsY, were studied using NMR and fluorescence spectroscopy. The A domain is mainly disordered and highly flexible; it binds to lipids via its N terminus and the C-terminal membrane targeting sequence. The central A domain binds to the translocon non-specifically and maintains disorder. Translocon targeting and binding of the A domain is driven by electrostatic interactions. The intrinsically disordered A domain tethers FtsY to the translocon, and because of its flexibility, allows the FtsY NG domain to scan a large area for binding to the NG domain of ribosome-bound SRP, thereby promoting the formation of the quaternary transfer complex at the membrane. PMID:27346853

  18. Strain Monitoring of Flexible Structures

    Science.gov (United States)

    Litteken, Douglas A.

    2017-01-01

    One of the biggest challenges facing NASA's deep space exploration goals is structural mass. A long duration transit vehicle on a journey to Mars, for example, requires a large internal volume for cargo, supplies and crew support. As with all space structures, a large pressure vessel is not enough. The vehicle also requires thermal, micro-meteoroid, and radiation protection, a navigation and control system, a propulsion system, and a power system, etc. As vehicles get larger, their associated systems also get larger and more complex. These vehicles require larger lift capacities and force the mission to become extremely costly. In order to build large volume habitable vehicles, with only minimal increases in launch volume and mass, NASA is developing lightweight structures. Lightweight structures are made from non-metallic materials including graphite composites and high strength fabrics and could provide similar or better structural capability than metals, but with significant launch volume and mass savings. Fabric structures specifically, have been worked by NASA off and on since its inception, but most notably in the 1990's with the TransHAB program. These TransHAB developed structures use a layered material approach to form a pressure vessel with integrated thermal and micro-meteoroid and orbital debris (MMOD) protection. The flexible fabrics allow the vessel to be packed in a small volume during launch and expand into a much larger volume once in orbit. NASA and Bigelow Aerospace recently installed the first human-rated inflatable module on the International Space Station (ISS), known as the Bigelow Expandable Activity Module (BEAM) in May of 2016. The module provides a similar internal volume to that of an Orbital ATK Cygnus cargo vehicle, but with a 77% launch volume savings. As lightweight structures are developed, testing methods are vital to understanding their behavior and validating analytical models. Common techniques can be applied to fabric materials

  19. Multisensory integration : flexible use of general operations

    NARCIS (Netherlands)

    Van Atteveldt, N.; Murray, Micah M; Thut, Gregor; Schroeder, Charles E

    2014-01-01

    Research into the anatomical substrates and "principles" for integrating inputs from separate sensory surfaces has yielded divergent findings. This suggests that multisensory integration is flexible and context dependent and underlines the need for dynamically adaptive neuronal integration mechanism

  20. Sources of Operational Flexibility, Greening the Grid

    Energy Technology Data Exchange (ETDEWEB)

    Katz, Jessica; Milligan, Michael; Cochran, Jaquelin

    2015-05-01

    Changes in electricity demand and generation must be constantly balanced to maintain power system stability and reliability. Operational flexibility refers to the ability of a power system to respond to these changes. Power systems are designed and operated to efficiently manage variability and uncertainty in electricity demand and resource availability. Variable renewable energy (VRE) increases this inherent variability and uncertainty, and thus increases the need for flexibility. Systems with significant variability and uncertainty require flexible generators that can rapidly change output, operate efficiently at lower outputs, and operate for short durations. This flexibility in turn can reduce the need to curtail (decrease the output of) solar and wind generation; improve investor confidence in VRE and revenue streams; decrease the risk of negative market pricing (which results when conventional generators cannot turn down to low outputs during times of high VRE output); and reduce environmental impacts by increasing system efficiency and maximizing the utilization of VRE.

  1. Fixed Price Dynamics versus Flexible Price Dynamics

    OpenAIRE

    Martin Currie; Ingrid Kubin

    2005-01-01

    This paper contrasts the dynamical behaviors of fixed and flexible price regimes for a monopolistically competitive manufacturing sector in which firms base decisions on expectations about product demands. (author's abstract)

  2. Piezoelectric RL shunt damping of flexible structures

    DEFF Research Database (Denmark)

    Høgsberg, Jan Becker; Krenk, Steen

    2015-01-01

    Resonant RL shunt circuits represent a robust and effective approach to piezoelectric damping, provided that the individual shunt circuit components are calibrated accurately with respect to the dynamic properties of the corresponding flexible structure. The balanced calibration procedure applied...

  3. Fully Integral, Flexible Composite Driveshaft Project

    Data.gov (United States)

    National Aeronautics and Space Administration — An all-composite driveshaft incorporating integral flexible diaphragms is described and proposed for phase II prime conractor testing. The approach obsoletes the...

  4. Thin Flexible IMM Solar Array Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Inverted Metamorphic (IMM) solar cells have achieved high efficiency at very low mass, but integration of the thin crystalline photovoltaic device into a flexible...

  5. Integrated flexible chalcogenide glass photonic devices

    Science.gov (United States)

    Li, Lan; Lin, Hongtao; Qiao, Shutao; Zou, Yi; Danto, Sylvain; Richardson, Kathleen; Musgraves, J. David; Lu, Nanshu; Hu, Juejun

    2014-08-01

    Photonic integration on thin flexible plastic substrates is important for emerging applications ranging from the realization of flexible interconnects to conformal sensors applied to the skin. Such devices are traditionally fabricated using pattern transfer, which is complicated and has limited integration capacity. Here, we report a convenient monolithic approach to realize flexible, integrated high-index-contrast chalcogenide glass photonic devices. By developing local neutral axis designs and suitable fabrication techniques, we realize a suite of photonic devices including waveguides, microdisk resonators, add-drop filters and photonic crystals that have excellent optical performance and mechanical flexibility, enabling repeated bending down to sub-millimetre radii without measurable performance degradation. The approach offers a facile fabrication route for three-dimensional high-index-contrast photonics that are difficult to create using traditional methods.

  6. Technical Design of Flexible Sustainable Energy Systems

    DEFF Research Database (Denmark)

    Lund, Henrik

    2003-01-01

    The paper presents technical designs of potential future flexible energy systems in Denmark, which will be able both to balance production and demand and to secure voltage and frequency requirements on the grid....

  7. Adaptive Control Strategies for Flexible Robotic Arm

    Science.gov (United States)

    Bialasiewicz, Jan T.

    1996-01-01

    The control problem of a flexible robotic arm has been investigated. The control strategies that have been developed have a wide application in approaching the general control problem of flexible space structures. The following control strategies have been developed and evaluated: neural self-tuning control algorithm, neural-network-based fuzzy logic control algorithm, and adaptive pole assignment algorithm. All of the above algorithms have been tested through computer simulation. In addition, the hardware implementation of a computer control system that controls the tip position of a flexible arm clamped on a rigid hub mounted directly on the vertical shaft of a dc motor, has been developed. An adaptive pole assignment algorithm has been applied to suppress vibrations of the described physical model of flexible robotic arm and has been successfully tested using this testbed.

  8. On Quantification of Flexibility in Power Systems

    DEFF Research Database (Denmark)

    Bucher, Matthias A.; Delikaraoglou, Stefanos; Heussen, Kai;

    2015-01-01

    Large scale integration of fluctuating and nondispatchablegeneration and variable transmission patterns inducehigh uncertainty in power system operation. In turn,transmission system operators (TSOs) need explicit informationabout available flexibility to maintain a desired reliability level ata...

  9. Flexible ultrasonic array sensors for health monitoring

    Science.gov (United States)

    Kobayashi, M.; Wu, K.-T.; Song, L.; Liu, Q.; Jen, C.-K.

    2007-04-01

    Flexible ultrasonic array transducers which can be attached to the desired structures or materials for nondestructive testing and structural health monitoring applications at room and elevated temperatures are developed. These flexible ultrasonic transducers (UTs) arrays consist of a thin polyimide membrane with a bottom electrode or stainless steel foil, a piezoelectric lead-zirconate-titanate (PZT) composite film and top electrodes. The flexibility is realized owing to the porosity of piezoelectric film and the thinness of substrate and electrodes. Top and bottom electrode materials are silver paste, silver paint or electroless plated nickel alloys. The UT array is configured by the several top electrodes. The flexible UT has been successfully tested at 150°C and also immersed into water as immersion ultrasonic probe operated in the pulse-echo mode with good signal to noise ratio.

  10. Fully Integral, Flexible Composite Driveshaft Project

    Data.gov (United States)

    National Aeronautics and Space Administration — An all-composite driveshaft incorporating integral flexible diaphragms is described and proposed for further refinement. An approach is explored which obsoletes the...

  11. Flexible Hall sensors based on graphene

    Science.gov (United States)

    Wang, Zhenxing; Shaygan, Mehrdad; Otto, Martin; Schall, Daniel; Neumaier, Daniel

    2016-03-01

    The excellent electronic and mechanical properties of graphene provide a perfect basis for high performance flexible electronic and sensor devices. Here, we present the fabrication and characterization of flexible graphene based Hall sensors. The Hall sensors are fabricated on 50 μm thick flexible Kapton foil using large scale graphene grown by chemical vapor deposition technique on copper foil. Voltage and current normalized sensitivities of up to 0.096 V VT-1 and 79 V AT-1 were measured, respectively. These values are comparable to the sensitivity of rigid silicon based Hall sensors and are the highest values reported so far for any flexible Hall sensor devices. The sensitivity of the Hall sensor shows no degradation after being bent to a minimum radius of 4 mm, which corresponds to a tensile strain of 0.6%, and after 1000 bending cycles to a radius of 5 mm.

  12. Thin Flexible IMM Solar Array Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Thin, flexible, and highly efficient solar arrays are needed that package compactly for launch and deploy into large, structurally stable high power generators....

  13. Carbon Nanotube Flexible and Stretchable Electronics

    Science.gov (United States)

    Cai, Le; Wang, Chuan

    2015-08-01

    The low-cost and large-area manufacturing of flexible and stretchable electronics using printing processes could radically change people's perspectives on electronics and substantially expand the spectrum of potential applications. Examples range from personalized wearable electronics to large-area smart wallpapers and from interactive bio-inspired robots to implantable health/medical apparatus. Owing to its one-dimensional structure and superior electrical property, carbon nanotube is one of the most promising material platforms for flexible and stretchable electronics. Here in this paper, we review the recent progress in this field. Applications of single-wall carbon nanotube networks as channel semiconductor in flexible thin-film transistors and integrated circuits, as stretchable conductors in various sensors, and as channel material in stretchable transistors will be discussed. Lastly, state-of-the-art advancement on printing process, which is ideal for large-scale fabrication of flexible and stretchable electronics, will also be reviewed in detail.

  14. Inhibition of selectin binding

    Energy Technology Data Exchange (ETDEWEB)

    Nagy, Jon O. (Rodeo, CA); Spevak, Wayne R. (Albany, CA); Dasgupta, Falguni (New Delhi, IN); Bertozzi, Caroline (Albany, CA)

    2001-10-09

    This invention provides compositions for inhibiting the binding between two cells, one expressing P- or L-selectin on the surface and the other expressing the corresponding ligand. A covalently crosslinked lipid composition is prepared having saccharides and acidic group on separate lipids. The composition is then interposed between the cells so as to inhibit binding. Inhibition can be achieved at an effective oligosaccharide concentration as low as 10.sup.6 fold below that of the free saccharide. Since selectins are involved in recruiting cells to sites of injury, these composition scan be used to palliate certain inflammatory and immunological conditions.

  15. Inhibition of selectin binding

    Energy Technology Data Exchange (ETDEWEB)

    Nagy, J.O.; Spevak, W.R.; Dasgupta, F.; Bertozzi, C.

    1999-10-05

    This invention provides a system for inhibiting the binding between two cells, one expressing P- or L-selectin on the surface and the other expressing the corresponding ligand. A covalently crosslinked lipid composition is prepared having saccharides and acidic group on separate lipids. The composition is then interposed between the cells so as to inhibit binding. Inhibition can be achieved at an effective oligosaccharide concentration as low as 10{sup 6} fold below that of the free saccharide. Since selectins are involved in recruiting cells to sites of injury, this system can be used to palliate certain inflammatory and immunological conditions.

  16. Inhibition of selectin binding

    Energy Technology Data Exchange (ETDEWEB)

    Nagy, Jon O. (Rodeo, CA); Spevak, Wayne R. (Albany, CA); Dasgupta, Falguni (New Delhi, IN); Bertozzi, Caroline (Albany, CA)

    1999-01-01

    This invention provides compositions for inhibiting the binding between two cells, one expressing P- or L-selectin on the surface and the other expressing the corresponding ligand. A covalently crosslinked lipid composition is prepared having saccharides and acidic group on separate lipids. The composition is then interposed between the cells so as to inhibit binding. Inhibition can be achieved at an effective oligosaccharide concentration as low as 10.sup.6 fold below that of the free saccharide. Since selectins are involved in recruiting cells to sites of injury, these composition scan be used to palliate certain inflammatory and immunological conditions.

  17. Inhibition of selectin binding

    Energy Technology Data Exchange (ETDEWEB)

    Nagy, J.O.; Spevak, W.R.; Dasgupta, F.; Bertozzi, C.

    1999-11-16

    This invention provides compositions for inhibiting the binding between two cells, one expressing P- or L-selectin on the surface and the other expressing the corresponding ligand. A covalently crosslinked lipid composition is prepared having saccharides and acidic group on separate lipids. The composition is then interposed between the cells so as to inhibit binding. Inhibition can be achieved at an effective oligosaccharide concentration as low as 10{sup 6} fold below that of the free saccharide. Since selectins are involved in recruiting cells to sites of injury, these composition scan be used to palliate certain inflammatory and immunological conditions.

  18. Inhibition of selectin binding

    Energy Technology Data Exchange (ETDEWEB)

    Nagy, Jon O. (Rodeo, CA); Spevak, Wayne R. (Albany, CA); Dasgupta, Falguni (New Delhi, IN); Bertozzi, Carolyn (Albany, CA)

    1999-10-05

    This invention provides a system for inhibiting the binding between two cells, one expressing P- or L-selectin on the surface and the other expressing the corresponding ligand. A covalently crosslinked lipid composition is prepared having saccharides and acidic group on separate lipids. The composition is then interposed between the cells so as to inhibit binding. Inhibition can be achieved at an effective oligosaccharide concentration as low as 10.sup.6 fold below that of the free saccharide. Since selectins are involved in recruiting cells to sites of injury, this system can be used to palliate certain inflammatory and immunological conditions.

  19. Binding-site assessment by virtual fragment screening.

    Directory of Open Access Journals (Sweden)

    Niu Huang

    Full Text Available The accurate prediction of protein druggability (propensity to bind high-affinity drug-like small molecules would greatly benefit the fields of chemical genomics and drug discovery. We have developed a novel approach to quantitatively assess protein druggability by computationally screening a fragment-like compound library. In analogy to NMR-based fragment screening, we dock approximately 11,000 fragments against a given binding site and compute a computational hit rate based on the fraction of molecules that exceed an empirically chosen score cutoff. We perform a large-scale evaluation of the approach on four datasets, totaling 152 binding sites. We demonstrate that computed hit rates correlate with hit rates measured experimentally in a previously published NMR-based screening method. Secondly, we show that the in silico fragment screening method can be used to distinguish known druggable and non-druggable targets, including both enzymes and protein-protein interaction sites. Finally, we explore the sensitivity of the results to different receptor conformations, including flexible protein-protein interaction sites. Besides its original aim to assess druggability of different protein targets, this method could be used to identifying druggable conformations of flexible binding site for lead discovery, and suggesting strategies for growing or joining initial fragment hits to obtain more potent inhibitors.

  20. Skills and Recruitment in Flexible Work Settings

    OpenAIRE

    Apitzsch, Birgit

    2012-01-01

    Over recent decades, we have witnessed profound changes in labour markets with an increase in flexible forms of work and employment, driven by organizational restructuring and institutional change at the macro- and at the sectoral level. In order to understand transformations of employment it is central to grasp the interrelations between the organisation of work, recruitment practices, and labour market institutions. Yet, despite the growing interest in flexible work practices and the growin...

  1. Biaxially oriented film on flexible polymeric substrate

    Science.gov (United States)

    Finkikoglu, Alp T.; Matias, Vladimir

    2009-10-13

    A flexible polymer-based template having a biaxially oriented film grown on the surface of a polymeric substrate. The template having the biaxially oriented film can be used for further epitaxial growth of films of interest for applications such as photovoltaic cells, light emitting diodes, and the like. Methods of forming such a flexible template and providing the polymeric substrate with a biaxially oriented film deposited thereon are also described.

  2. Consumption Flexibility, Product Configuration, and Market Competition

    OpenAIRE

    Liang Guo

    2006-01-01

    When purchase and consumption decisions are separated in time and when future utility is state dependent, consumers may desire to pursue consumption flexibility by purchasing different products together (multiple buying). This paper analyzes the effects of consumption flexibility on competing firms' marketing mix decisions, in a model in which future preference uncertainty exists and consumers differ in their preferred product location on a horizontal attribute. The analysis shows that the na...

  3. Flexible Employment Arrangements and Workplace Performance

    OpenAIRE

    Giovanis, Eleftherios

    2016-01-01

    There is an increasing concern on the quality of jobs and productivity that is witnessed in the flexible employment arrangements. However, the effects of the employment flexible types on workplace performance has not been explored in Britain. In this study the relationship between two employment arrangements and the workplace performance is examined. More specifically, teleworking and compress hours are two main employment types examined using the Workplace Employee R...

  4. Spacecraft Dynamic Characteristics While Deploying Flexible Beams

    Institute of Scientific and Technical Information of China (English)

    程绪铎; 李俊峰; 樊勇; 王照林

    2002-01-01

    The attitude dynamic equations of a spacecraft while deploying two flexible beams and the beam equations were developed from momentum theory. The dynamic equations were solved numerically using the Runge-Kutta method to calculate the vibration amplitudes of the flexible beams and the attitude angular velocity. The results show that the vibration amplitudes increase as the beam length increases or as the initial attitude angular velocity increases. The results also show that the vibration amplitudes decrease as the deployment velocity increases.

  5. Flexible receiver adapter formal design review

    International Nuclear Information System (INIS)

    This memo summarizes the results of the Formal (90%) Design Review process and meetings held to evaluate the design of the Flexible Receiver Adapters, support platforms, and associated equipment. The equipment is part of the Flexible Receiver System used to remove, transport, and store long length contaminated equipment and components from both the double and single-shell underground storage tanks at the 200 area tank farms

  6. Capturing Aggregate Flexibility in Demand Response

    OpenAIRE

    Alizadeh, Mahnoosh; Scaglione, Anna; Goldsmith, Andrea; Kesidis, George

    2014-01-01

    Flexibility in electric power consumption can be leveraged by Demand Response (DR) programs. The goal of this paper is to systematically capture the inherent aggregate flexibility of a population of appliances. We do so by clustering individual loads based on their characteristics and service constraints. We highlight the challenges associated with learning the customer response to economic incentives while applying demand side management to heterogeneous appliances. We also develop a framewo...

  7. Correlated Flexible Molecular Coding and Molecular Evolvability

    OpenAIRE

    Husimi, Y; Aita, T.; Tabuchi, I.

    2002-01-01

    Evolvability of biopolymers is based on molecular coding. The molecular coding is represented by biopolymer function vs monomeric sequence relationship, that is, a proper fitness landscape on the sequence space. On the other hand, molecular coding is mostly realized by monomeric sequence vs biopolymer structure relationship. We suggest the evolution of evolvability based on flexible or multiplex coding originating from flexible or polymorphic conformation of evolving biopolymers. We report a ...

  8. Composite materials in flexible multibody systems

    OpenAIRE

    Neto, Maria Augusta; Ambrósio, Jorge A. C.; Leal, Rogério P.

    2006-01-01

    In this work the flexible multibody dynamics formulations of complex models are extended to include elastic components made of laminated composite materials. The only limitation for the deformation of a structural member is that it must be elastic and linear when described in a body fixed frame. A finite element model for each flexible body is obtained such that the nodal coordinates are described with respect to the body fixed frame and the inertia terms involved in the mass matrix and gyros...

  9. Cognitive flexibility predicts early reading skills

    OpenAIRE

    LynneG.Duncan; PascaleColé

    2014-01-01

    An important aspect of learning to read is efficiency in accessing different kinds of linguistic information (orthographic, phonological and semantic) about written words. The present study investigates whether, in addition to the integrity of such linguistic skills, early progress in reading may require a degree of cognitive flexibility in order to manage the coordination of this information effectively. Our study will look for evidence of a link between flexibility and both word reading and...

  10. Achieving Business Process Flexibility with Business Rules

    OpenAIRE

    Eijndhoven, van, SJL Stef; Iacob, M.E.; Ponisio, M.L.

    2008-01-01

    Business processes used in networked business are often large and complex, which makes them difficult to manage and change. In this paper we address this lack of flexibility by proposing a solution that uses business rules and workflow patterns to model the variable parts of process flow, thus facilitating dynamic pattern composition in these areas. We argue that the increase in flexibility is justified by the fact that changes in a business process can be confined to the variable isolated pa...

  11. Agilo: A Highly Flexible Groupware Framework

    OpenAIRE

    Guicking, Axel; Tandler, Peter; Avgeriou, Paris

    2005-01-01

    Today there exist many frameworks for the development of synchronous groupware applications. Although the domain of these applications is very heterogeneous, existing frameworks provide only limited flexibility to integrate diverse groupware applications in a meaningful way. We identify five variation points that a groupware framework needs to offer in a flexible way in order to facilitate the integration of diverse groupware applications. Based on these variation points, we propose a groupwa...

  12. Entry and exit decisions in flexible teams

    OpenAIRE

    Michael Christensen; Thorbjørn Knudsen

    2008-01-01

    The present study identifies a major limitation of current research on multinational enterprises (MNEs). Joint decision-making in a distributed setting is of critical importance in practice, but has received little attention in our theories. To address this gap in knowledge, we examine the effects of flexible decision teams when MNEs assess turbulent markets. Remarkably, flexible teams composed of fallible evaluators can outperform what is usually thought of as an “optimal” decision. Our main...

  13. Flexibility in natural gas supply and demand

    OpenAIRE

    Cornot-Gandolphe, Sylvie

    2015-01-01

    "In most IEA Member countries, natural gas demand varies strongly during the year, according to temperature. Flexibility is needed to cover seasonal swings and variations in gas demand, especially for household customers. It is also needed to maintain short-term supply security in the case of a disruption of a supply source. Over the years, gas companies have devised a variety of flexibility tools, such as supply swing, storage and interruptible contracts. This enabled them to balance supply ...

  14. Stochastic Dynamics Underlying Cognitive Stability and Flexibility

    OpenAIRE

    Kai Ueltzhöffer; Armbruster-Genç, Diana J. N.; Christian J Fiebach

    2015-01-01

    Cognitive stability and flexibility are core functions in the successful pursuit of behavioral goals. While there is evidence for a common frontoparietal network underlying both functions and for a key role of dopamine in the modulation of flexible versus stable behavior, the exact neurocomputational mechanisms underlying those executive functions and their adaptation to environmental demands are still unclear. In this work we study the neurocomputational mechanisms underlying cue based task ...

  15. External Job Churning and Internal Job Flexibility

    OpenAIRE

    Peter Cappelli; David Neumark

    2001-01-01

    Concern about job instability and insecurity has a long history and has generated a considerable body of research across the social sciences, most recently focused on whether job stability and security have declined. Internally flexible systems for organizing work, sometimes called 'functionally flexible' systems, have been proposed as arrangements that can reduce job instability and insecurity by reducing the need for firms to rely on job cuts or contingent work to be able to respond to chan...

  16. Flexible thin-film planar peltier microcooler

    OpenAIRE

    L.M. Gonçalves; Couto, Carlos; Correia, J.H.; Alpuim, P.; Min, Gao; Rowe, D. M.

    2006-01-01

    The present work reports on the fabrication and characterization of the first planar Peltier microcooler on a flexible substrate. The microcooler was fabricated on flexible Kapton© polyimide substrate, 25 μm in thickness, using Bi2Te3 and Sb2Te3 thermoelectric elements, and was deposited by thermal co-evaporation. Portuguese Foundation for Science and Technology (SFRH/BD/18142/2004) Agência de Inovação (POCTI MPYROM)

  17. A Flexible Nurse Scheduling Support System

    OpenAIRE

    Ozkarahan, Irem

    1987-01-01

    Scheduling nursing personnel in hospitals is very complex because of the variety of conflicting interests and objectives. Also, demand varies 24-hour a day 7-day a week, is skill specific and hard to forecast. In the face of this complexity, the present nurse scheduling models have met with little success. In this paper, we propose a more flexible decision support system that will satisfy the interests of both hospitals and nurses through alternative models that attempt to accommodate flexibl...

  18. Access to flexible working and informal care

    OpenAIRE

    Bryan, Mark L.

    2011-01-01

    We use matched employer-employee data to explore the relationship between employees' access to flexible working arrangements and the amount of informal care they provide to sick or elderly friends and relatives. Flexitime and the ability to reduce working hours are each associated with about 10% more hours of informal care, with effects concentrated among full-time workers providing small amounts of care. The wider workplace environment beyond formal flexible work also appears to facilitate c...

  19. Fast Flexible Transistors with a Nanotrench Structure

    OpenAIRE

    Jung-Hun Seo; Tao Ling; Shaoqin Gong; Weidong Zhou; Alice L. Ma; L. Jay Guo; Zhenqiang Ma

    2016-01-01

    The simplification of fabrication processes that can define very fine patterns for large-area flexible radio-frequency (RF) applications is very desirable because it is generally very challenging to realize submicron scale patterns on flexible substrates. Conventional nanoscale patterning methods, such as e-beam lithography, cannot be easily applied to such applications. On the other hand, recent advances in nanoimprinting lithography (NIL) may enable the fabrication of large-area nanoelectro...

  20. Spatial operator algebra for flexible multibody dynamics

    Science.gov (United States)

    Jain, A.; Rodriguez, G.

    1993-01-01

    This paper presents an approach to modeling the dynamics of flexible multibody systems such as flexible spacecraft and limber space robotic systems. A large number of degrees of freedom and complex dynamic interactions are typical in these systems. This paper uses spatial operators to develop efficient recursive algorithms for the dynamics of these systems. This approach very efficiently manages complexity by means of a hierarchy of mathematical operations.

  1. Instant Attraction: Immediate Action-Effect Bindings Occur for Both, Stimulus- and Goal-Driven Actions

    OpenAIRE

    Janczyk, Markus; Heinemann, Alexander; Pfister, Roland

    2012-01-01

    Flexible behavior is only possible if contingencies between own actions and following environmental effects are acquired as quickly as possible; and recent findings indeed point toward an immediate formation of action-effect bindings already after a single coupling of an action and its effect. The present study explored whether these short-term bindings occur for both, stimulus- and goal-driven actions (“forced-choice actions” vs. “free-choice actions”). Two experiments confirmed that immedia...

  2. Flexible ERP: Enterprise Information System Under the Agile Manufacture Mode

    Institute of Scientific and Technical Information of China (English)

    SU Xuanliang; CHENG Guoping; WANG Mingquan

    2006-01-01

    Flexibility is the demand of management revolution, and also the milestone of management development. The flexible ERP system is the information system to orientate variety and handle uncertainties. The market with rapid change and agile manufacture need a flexible ERP system.The article elaborates the meanings and characteristics about flexibility, the flexibility of ERP and the flexible ERP system; it discussed the necessity, the possibility and the related condition of setting up the flexible ERP system; and puts forward the strategy and the approaches of setting up the flexible ERP system.

  3. Enabling Technologies for Fabrication of Large Area Flexible Antennas Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Flexible, foldable, and/or inflatable antenna systems open up a wealth of opportunities. Integrating antenna elements and related electronics onto flexible...

  4. Flexible Thermoelectric Generators on Silicon Fabric

    KAUST Repository

    Sevilla, Galo T.

    2012-11-01

    In this work, the development of a Thermoelectric Generator on Flexible Silicon Fabric is explored to extend silicon electronics for flexible platforms. Low cost, easily deployable plastic based flexible electronics are of great interest for smart textile, wearable electronics and many other exciting applications. However, low thermal budget processing and fundamentally limited electron mobility hinders its potential to be competitive with well established and highly developed silicon technology. The use of silicon in flexible electronics involve expensive and abrasive materials and processes. In this work, high performance flexible thermoelectric energy harvesters are demonstrated from low cost bulk silicon (100) wafers. The fabrication of the micro- harvesters was done using existing silicon processes on silicon (100) and then peeled them off from the original substrate leaving it for reuse. Peeled off silicon has 3.6% thickness of bulk silicon reducing the thermal loss significantly and generating nearly 30% more output power than unpeeled harvesters. The demonstrated generic batch processing shows a pragmatic way of peeling off a whole silicon circuitry after conventional fabrication on bulk silicon wafers for extremely deformable high performance integrated electronics. In summary, by using a novel, low cost process, this work has successfully integrated existing and highly developed fabrication techniques to introduce a flexible energy harvester for sustainable applications.

  5. Effect of nozzle flexibility on piping system

    International Nuclear Information System (INIS)

    Piping systems should be designed flexibly to avoid the burden more on the buffer tube and nozzle equipment. In conditions where space is limited then to create a routing that is flexible enough not impossible to achieve. As a result, the force generated would be enormous. One way to reduce the load more on the support and on the equipment nozzles with the method of nozzle flexibility. In this method, the nozzle was not considered to be rigid but has a certain flexibility in accordance with the dimensions of the equipment. Based on calculations using Caesar II produced Fx = 11926 kg, Fy = 2510 kg, FZ = 800 kg, Mx = -17712 kg-m, My = 44514 kg-m and Mz = 7813 kg-m. From these data there are still components that moment, that is My still quite large. To reduce the load on the nozzle so on nozzle factors included flexibility in order to get results Fx = 1989 kg, Fy = -1938, FZ = 1672, Mx = -1798 kg-m, My = 8106 kg-m and Mz = 3846 kg-m . Seeing the results of calculations with the nozzle flexibility and style of the moment turns out that there is less than the allowable limits so that this condition is declared safe to install. (author)

  6. Fast Flexible Transistors with a Nanotrench Structure

    Science.gov (United States)

    Seo, Jung-Hun; Ling, Tao; Gong, Shaoqin; Zhou, Weidong; Ma, Alice L.; Guo, L. Jay; Ma, Zhenqiang

    2016-04-01

    The simplification of fabrication processes that can define very fine patterns for large-area flexible radio-frequency (RF) applications is very desirable because it is generally very challenging to realize submicron scale patterns on flexible substrates. Conventional nanoscale patterning methods, such as e-beam lithography, cannot be easily applied to such applications. On the other hand, recent advances in nanoimprinting lithography (NIL) may enable the fabrication of large-area nanoelectronics, especially flexible RF electronics with finely defined patterns, thereby significantly broadening RF applications. Here we report a generic strategy for fabricating high-performance flexible Si nanomembrane (NM)-based RF thin-film transistors (TFTs), capable of over 100 GHz operation in theory, with NIL patterned deep-submicron-scale channel lengths. A unique 3-dimensional etched-trench-channel configuration was used to allow for TFT fabrication compatible with flexible substrates. Optimal device parameters were obtained through device simulation to understand the underlying device physics and to enhance device controllability. Experimentally, a record-breaking 38 GHz maximum oscillation frequency fmax value has been successfully demonstrated from TFTs with a 2 μm gate length built with flexible Si NM on plastic substrates.

  7. Multibody Dynamics of Very Flexible Damped Systems

    International Nuclear Information System (INIS)

    An efficient multibody dynamics formulation is presented for simulating the forward dynamics of open and closed loop mechanical systems comprised of rigid and flexible bodies interconnected by revolute, prismatic, free, and fixed joints. Geometrically nonlinear deformation of flexible bodies is included and the formulation does not impose restrictions on the representation of material damping within flexible bodies. The approach is based on Kane's equation without multipliers and the resulting formulation generates 2ndof+m first order ordinary differential equations directly where 2nd of is the smallest number of system degrees of freedom that can completely describe the system configuration and m is the number of loop closure velocity constraint equations. The equations are integrated numerically in the time domain to propagate the solution. Flexible bodies are discretized using a finite element approach. The mass and stiffness matrices for a six-degree-of-freedom planar beam element are developed including mass coupling terms, rotary inertia, centripetal and Coriolis forces, and geometric stiffening terms. The formulation is implemented in the general purpose multibody dynamics computer program 'flxdyn'. Extensive validation of the formulation and corresponding computer program is accomplished by comparing results with analytically derived equations, alternative approximate solutions, and benchmark problems selected from the literature. The formulation is found to perform well in terms of accuracy and solution efficiency. This article develops the formulation and presents a set of validation problems including a sliding pendulum, seven link mechanism, flexible beam spin-up problem, and flexible slider crank mechanism

  8. Multibody Dynamics of Very Flexible Damped Systems

    Energy Technology Data Exchange (ETDEWEB)

    Langlois, R.G.; Anderson, R.J. [Queen' s University at Kingston, Department of Mechanical Engineering (Canada)

    1999-05-15

    An efficient multibody dynamics formulation is presented for simulating the forward dynamics of open and closed loop mechanical systems comprised of rigid and flexible bodies interconnected by revolute, prismatic, free, and fixed joints. Geometrically nonlinear deformation of flexible bodies is included and the formulation does not impose restrictions on the representation of material damping within flexible bodies. The approach is based on Kane's equation without multipliers and the resulting formulation generates 2ndof+m first order ordinary differential equations directly where 2nd of is the smallest number of system degrees of freedom that can completely describe the system configuration and m is the number of loop closure velocity constraint equations. The equations are integrated numerically in the time domain to propagate the solution. Flexible bodies are discretized using a finite element approach. The mass and stiffness matrices for a six-degree-of-freedom planar beam element are developed including mass coupling terms, rotary inertia, centripetal and Coriolis forces, and geometric stiffening terms. The formulation is implemented in the general purpose multibody dynamics computer program 'flxdyn'. Extensive validation of the formulation and corresponding computer program is accomplished by comparing results with analytically derived equations, alternative approximate solutions, and benchmark problems selected from the literature. The formulation is found to perform well in terms of accuracy and solution efficiency. This article develops the formulation and presents a set of validation problems including a sliding pendulum, seven link mechanism, flexible beam spin-up problem, and flexible slider crank mechanism.

  9. Fast Flexible Transistors with a Nanotrench Structure.

    Science.gov (United States)

    Seo, Jung-Hun; Ling, Tao; Gong, Shaoqin; Zhou, Weidong; Ma, Alice L; Guo, L Jay; Ma, Zhenqiang

    2016-01-01

    The simplification of fabrication processes that can define very fine patterns for large-area flexible radio-frequency (RF) applications is very desirable because it is generally very challenging to realize submicron scale patterns on flexible substrates. Conventional nanoscale patterning methods, such as e-beam lithography, cannot be easily applied to such applications. On the other hand, recent advances in nanoimprinting lithography (NIL) may enable the fabrication of large-area nanoelectronics, especially flexible RF electronics with finely defined patterns, thereby significantly broadening RF applications. Here we report a generic strategy for fabricating high-performance flexible Si nanomembrane (NM)-based RF thin-film transistors (TFTs), capable of over 100 GHz operation in theory, with NIL patterned deep-submicron-scale channel lengths. A unique 3-dimensional etched-trench-channel configuration was used to allow for TFT fabrication compatible with flexible substrates. Optimal device parameters were obtained through device simulation to understand the underlying device physics and to enhance device controllability. Experimentally, a record-breaking 38 GHz maximum oscillation frequency fmax value has been successfully demonstrated from TFTs with a 2 μm gate length built with flexible Si NM on plastic substrates. PMID:27094686

  10. Flexible conductive nanocellulose combined with silicon nanoparticles and polyaniline.

    Science.gov (United States)

    Park, Minsung; Lee, Dajung; Shin, Sungchul; Kim, Hyun-Joong; Hyun, Jinho

    2016-04-20

    Here we describe a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline. BC was used as a template for binding SiNPs resulting in a very promising anode material for Li-ion rechargeable batteries that showed a high specific capacity. The surfaces of the SiNPs were modified with phytic acid to enhance the binding of aniline monomer to the surface. A conformal coating of polyaniline (PANi) was formed on the modified SiNPs by in situ polymerization of aniline monomers. We also found that the phytic acid on the SiNPs was critical to ensure encapsulation of SiNPs with PANi. In addition, the phosphoric acid-tagged surface of the SiNPs enhanced the adhesion of SiNPs to the BC fibers. The resulting three dimensional network of BC was flexible and provided stress dissipation in the conductive BC composites. Flexural testing of conductive BC composites showed stable electrical conductivity even after repetitive bending over 100 times. PMID:26876826

  11. Flexible Metal Oxide/Graphene Oxide Hybrid Neuromorphic Transistors on Flexible Conducting Graphene Substrates.

    Science.gov (United States)

    Wan, Chang Jin; Liu, Yang Hui; Feng, Ping; Wang, Wei; Zhu, Li Qiang; Liu, Zhao Ping; Shi, Yi; Wan, Qing

    2016-07-01

    Flexible metal oxide/graphene oxide hybrid multi-gate neuromorphic transistors are fabricated on flexible conducting graphene substrates. Dendritic integrations in both spatial and temporal modes are emulated, and spatiotemporal correlated logics are obtained. A proof-of-principle visual system model for emulating Lobula Giant Motion Detector neuron is also investigated. The results are of great significance for flexible sensors and neuromorphic cognitive systems. PMID:27159546

  12. Sequential memory: Binding dynamics

    Science.gov (United States)

    Afraimovich, Valentin; Gong, Xue; Rabinovich, Mikhail

    2015-10-01

    Temporal order memories are critical for everyday animal and human functioning. Experiments and our own experience show that the binding or association of various features of an event together and the maintaining of multimodality events in sequential order are the key components of any sequential memories—episodic, semantic, working, etc. We study a robustness of binding sequential dynamics based on our previously introduced model in the form of generalized Lotka-Volterra equations. In the phase space of the model, there exists a multi-dimensional binding heteroclinic network consisting of saddle equilibrium points and heteroclinic trajectories joining them. We prove here the robustness of the binding sequential dynamics, i.e., the feasibility phenomenon for coupled heteroclinic networks: for each collection of successive heteroclinic trajectories inside the unified networks, there is an open set of initial points such that the trajectory going through each of them follows the prescribed collection staying in a small neighborhood of it. We show also that the symbolic complexity function of the system restricted to this neighborhood is a polynomial of degree L - 1, where L is the number of modalities.

  13. Probabilistic inference of transcription factor binding from multiple data sources.

    Science.gov (United States)

    Lähdesmäki, Harri; Rust, Alistair G; Shmulevich, Ilya

    2008-01-01

    An important problem in molecular biology is to build a complete understanding of transcriptional regulatory processes in the cell. We have developed a flexible, probabilistic framework to predict TF binding from multiple data sources that differs from the standard hypothesis testing (scanning) methods in several ways. Our probabilistic modeling framework estimates the probability of binding and, thus, naturally reflects our degree of belief in binding. Probabilistic modeling also allows for easy and systematic integration of our binding predictions into other probabilistic modeling methods, such as expression-based gene network inference. The method answers the question of whether the whole analyzed promoter has a binding site, but can also be extended to estimate the binding probability at each nucleotide position. Further, we introduce an extension to model combinatorial regulation by several TFs. Most importantly, the proposed methods can make principled probabilistic inference from multiple evidence sources, such as, multiple statistical models (motifs) of the TFs, evolutionary conservation, regulatory potential, CpG islands, nucleosome positioning, DNase hypersensitive sites, ChIP-chip binding segments and other (prior) sequence-based biological knowledge. We developed both a likelihood and a Bayesian method, where the latter is implemented with a Markov chain Monte Carlo algorithm. Results on a carefully constructed test set from the mouse genome demonstrate that principled data fusion can significantly improve the performance of TF binding prediction methods. We also applied the probabilistic modeling framework to all promoters in the mouse genome and the results indicate a sparse connectivity between transcriptional regulators and their target promoters. To facilitate analysis of other sequences and additional data, we have developed an on-line web tool, ProbTF, which implements our probabilistic TF binding prediction method using multiple data sources

  14. Increasing flexibility of assets. Transition to flexible assets; Die steigende Bedeutung der Kraftwerksparkflexibilitaet

    Energy Technology Data Exchange (ETDEWEB)

    Aumueller, Adolf; Moik, Albert [EVN AG, Maria Enzersdorf (Austria); Rall, Hueseyin [STEAG GmbH, Herne (Germany)

    2016-07-01

    For the Energiewende flexibility is the key for the security of supply during the transition to an energy system based on renewables as well as for balancing the volatile generation in a renewable energy system. Storage is an integral module for delivering the flexibility needed by the future power system. Remaining R and D for storages and significant concession and building time for pump storage power plants lead to a time gap until storage is able to bear the brunt of delivering flexibility. The efficient and effective during the transition phase is the use of existing flexible power plants.

  15. Discovery of 12-mer peptides that bind to wood lignin.

    Science.gov (United States)

    Yamaguchi, Asako; Isozaki, Katsuhiro; Nakamura, Masaharu; Takaya, Hikaru; Watanabe, Takashi

    2016-01-01

    Lignin, an abundant terrestrial polymer, is the only large-volume renewable feedstock composed of an aromatic skeleton. Lignin has been used mostly as an energy source during paper production; however, recent interest in replacing fossil fuels with renewable resources has highlighted its potential value in providing aromatic chemicals. Highly selective degradation of lignin is pivotal for industrial production of paper, biofuels, chemicals, and materials. However, few studies have examined natural and synthetic molecular components recognizing the heterogeneous aromatic polymer. Here, we report the first identification of lignin-binding peptides possessing characteristic sequences using a phage display technique. The consensus sequence HFPSP was found in several lignin-binding peptides, and the outer amino acid sequence affected the binding affinity of the peptides. Substitution of phenylalanine7 with Ile in the lignin-binding peptide C416 (HFPSPIFQRHSH) decreased the affinity of the peptide for softwood lignin without changing its affinity for hardwood lignin, indicating that C416 recognised structural differences between the lignins. Circular dichroism spectroscopy demonstrated that this peptide adopted a highly flexible random coil structure, allowing key residues to be appropriately arranged in relation to the binding site in lignin. These results provide a useful platform for designing synthetic and biological catalysts selectively bind to lignin. PMID:26903196

  16. Research into Flexibility Services. Final Report

    International Nuclear Information System (INIS)

    The Dutch Office for Energy Regulation (DTe) is currently investigating the Dutch gas flexibility market. DTe is concerned that Gasunie is dominant in the market. In order to take a view of Gasunie's market position, DTe needs to first define the market for gas flexibility services and then explore whether Gasunie is dominant in the market (or markets). DTe has commissioned Frontier to undertake the respective formal analysis. This report summarises the findings by Frontier. On the basis of this report and a formal consultation process, We follow a three-step approach to the study: (1) We first define the relevant markets for gas flexibility (Section 3); (2) We then analyse the structure of the markets for flexibility that we have defined (Section 4); (3) Finally, we assess whether Gasunie is dominant in the relevant markets, taking account of market shares and other competitive effects (Section 5). This document is the Final Report, which contains our views as to the market definition for gas flexibility and the position of Gasunie in the market. The remainder of this document is set out as follows: Section 2 provides an overview of aspects of the Dutch gas industry relevant to this study; Section 3 sets out our approach to defining the market and de-Mops our conclusions on the markets for gas flexibility; Section 4 provides our view as to the structure of the relevant flexibility markets as defined in Section 3; Section 5 reports our assessment as to whether Gasunie is dominant in the relevant markets, taking account of market shares and other competitive effects; Section 6 sets out our conclusions about the competitive assessment. We include three annexes that set out details related to the market definition and analysis of dominance

  17. Combinatorial Synthesis, Screening, and Binding Studies of Highly Functionalized Polyamino-amido Oligomers for Binding to Folded RNA

    Directory of Open Access Journals (Sweden)

    Jonathan K. Pokorski

    2012-01-01

    Full Text Available Folded RNA molecules have recently emerged as critical regulatory elements in biological pathways, serving not just as carriers of genetic information but also as key components in enzymatic assemblies. In particular, the transactivation response element (TAR of the HIV genome regulates transcriptional elongation by interacting specifically with the Tat protein, initiating the recruitment of the elongation complex. Preventing this interaction from occurring in vivo halts HIV replication, thus making RNA-binding molecules an intriguing pharmaceutical target. Using α-amino acids as starting materials, we have designed and synthesized a new class of polyamino-amido oligomers, called PAAs, specifically for binding to folded RNA structures. The PAA monomers were readily incorporated into a 125-member combinatorial library of PAA trimers. In order to rapidly assess RNA binding, a quantum dot-based fluorescent screen was developed to visualize RNA binding on-resin. The binding affinities of hits were quantified using a terbium footprinting assay, allowing us to identify a ligand (SFF with low micromolar affinity (kd=14 μM for TAR RNA. The work presented herein represents the development of a flexible scaffold that can be easily synthesized, screened, and subsequently modified to provide ligands specific for binding to folded RNAs.

  18. Flexible display technology for the objective force

    Science.gov (United States)

    Pellegrino, John M.; Wood, Gary L.; Morton, David C.; Forsythe, Eric W.; Girolamo, Henry J.

    2003-09-01

    The Army has initiated a flexible display research program. This program is part of the Army's strategy to create technologies that will enable revolutionary designs and transformational weapons systems for the Objective Force. The ARL flexible display program is more general than just for the dismounted soldier-it will also have implications for air and vehicular crews, and for the other services, even though it is not a DoD tri-service program per se. "Flexible" is defined in the program as displays that operate under conditions from conformal applications, limited flexing, to rollable displays for compact storage. The Army program will include display manufacturing concepts that enable roll-to-roll processes reduce cost, to tap a strong U.S. domestic industrial strength, and, eventually, to enable fabrication of very large sizes. There is commercial interest in flexible displays for applications ranging from wearable electronics for road-warriors and gamers to large screen 71-in. 1920x1200 color pixel consumer high definition television sets for walls. Industry willingness to cost share will be a key criterion in identifying investment opportunities that are necessary and timely from among all that may be envisioned. Some anticipated military applications and a roadmap are presented that identify the technology barriers at the materials, device and manufacturing levels to the creation of flexible display technology.

  19. Fault-based analysis of flexible ciphers

    Directory of Open Access Journals (Sweden)

    V.I.Korjik

    2002-07-01

    Full Text Available We consider security of some flexible ciphers against differential fault analysis (DFA. We present a description of the fault-based attack on two kinds of the flexible ciphers. The first kind is represented by the fast software-oriented cipher based on data-dependent subkey selection (DDSS, in which flexibility corresponds to the use of key-dependent operations. The second kind is represented by a DES-like cryptosystem GOST with secrete S-boxes. In general, the use of some secrete operations and procedures contributes to the security of the cryptosystem, however degree of this contribution depends significantly on the structure of the encryption mechanism. It is shown how to attack the DDSS-based flexible cipher using DFA though this cipher is secure against standard variants of the differential and linear cryptanalysis. We also give an outline of ciphers RC5 and GOST showing that they are also insecure against DFA-based attack. We suggest also a modification of the DDSS mechanism and a variant of the advanced DDSS-based flexible cipher that is secure against attacks based on random hardware faults.

  20. Recent progress in flexible OLED displays

    Science.gov (United States)

    Hack, Michael G.; Weaver, Michael S.; Mahon, Janice K.; Brown, Julie J.

    2001-09-01

    Organic light emitting device (OLED) technology has recently been shown to demonstrate excellent performance and cost characteristics for use in numerous flat panel display (FPD) applications. OLED displays emit bright, colorful light with excellent power efficiency, wide viewing angle and video response rates. OLEDs are also demonstrating the requisite environmental robustness for a wide variety of applications. OLED technology is also the first FPD technology with the potential to be highly functional and durable in a flexible format. The use of plastic and other flexible substrate materials offers numerous advantages over commonly used glass substrates, including impact resistance, light weight, thinness and conformability. Currently, OLED displays are being fabricated on rigid substrates, such as glass or silicon wafers. At Universal Display Corporation (UDC), we are developing a new class of flexible OLED displays (FOLEDs). These displays also have extremely low power consumption through the use of electrophosphorescent doped OLEDs. To commercialize FOLED technology, a number of technical issues related to packaging and display processing on flexible substrates need to be addressed. In this paper, we report on our recent results to demonstrate the key technologies that enable the manufacture of power efficient, long-life flexible OLED displays for commercial and military applications.

  1. Flexible barrier technology for enabling rollable AMOLED displays and upscaling flexible OLED lighting

    NARCIS (Netherlands)

    Li, F.M.; Unnikrishnan, S.; Weijer, P. van de; Assche, F. van; Shen, J.; Ellis, T.; Manders, W.; Akkerman, H.; Bouten, P.; Mol, A.M.B. van

    2013-01-01

    The availability of a high performance thin-film barrier is the most critical challenge in upscaling and commercializing flexible OLED products. We report a flexible thin-film-barrier technology that meets lifetime specifications for OLED lighting, and demonstrate it in rollable QVGA a-IGZO AMOLED d

  2. Flexible Programmes in Higher Professional Education: Expert Validation of a Flexible Educational Model

    Science.gov (United States)

    Schellekens, Ad; Paas, Fred; Verbraeck, Alexander; van Merrienboer, Jeroen J. G.

    2010-01-01

    In a preceding case study, a process-focused demand-driven approach for organising flexible educational programmes in higher professional education (HPE) was developed. Operations management and instructional design contributed to designing a flexible educational model by means of discrete-event simulation. Educational experts validated the model…

  3. Flexible Pedagogies: Employer Engagement and Work-Based Learning. Flexible Pedagogies: Preparing for the Future Series

    Science.gov (United States)

    Kettle, Jane

    2013-01-01

    This publication focuses on national and international policy initiatives to develop a better understanding of work-based learners and the types of flexibility that may well enhance their study especially pedagogically. As part of our five-strand research project "Flexible Pedagogies: preparing for the future" it: (1) highlights the…

  4. 12 CFR 742.1 - Regulatory Flexibility Program.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Regulatory Flexibility Program. 742.1 Section 742.1 Banks and Banking NATIONAL CREDIT UNION ADMINISTRATION REGULATIONS AFFECTING CREDIT UNIONS REGULATORY FLEXIBILITY PROGRAM § 742.1 Regulatory Flexibility Program. NCUA's Regulatory Flexibility Program (RegFlex) exempts from all or part of...

  5. Experimental validation of flexible robot arm modeling and control

    Science.gov (United States)

    Ulsoy, A. Galip

    1989-01-01

    Flexibility is important for high speed, high precision operation of lightweight manipulators. Accurate dynamic modeling of flexible robot arms is needed. Previous work has mostly been based on linear elasticity with prescribed rigid body motions (i.e., no effect of flexible motion on rigid body motion). Little or no experimental validation of dynamic models for flexible arms is available. Experimental results are also limited for flexible arm control. Researchers include the effects of prismatic as well as revolute joints. They investigate the effect of full coupling between the rigid and flexible motions, and of axial shortening, and consider the control of flexible arms using only additional sensors.

  6. Reflection-Based Python-C++ Bindings

    International Nuclear Information System (INIS)

    Python is a flexible, powerful, high-level language with excellent interactive and introspective capabilities and a very clean syntax. As such, it can be a very effective tool for driving physics analysis. Python is designed to be extensible in low-level C-like languages, and its use as a scientific steering language has become quite widespread. To this end, existing and custom-written C or C++ libraries are bound to the Python environment as so-called extension modules. A number of tools for easing the process of creating such bindings exist, such as SWIG and Boost. Python. Yet, the process still requires a considerable amount of effort and expertise. The C++ language has few built-in introspective capabilities, but tools such as LCGDict and CINT add this by providing so-called dictionaries: libraries that contain information about the names, entry points, argument types, etc. of other libraries. The reflection information from these dictionaries can be used for the creation of bindings and so the process can be fully automated, as dictionaries are already provided for many end-user libraries for other purposes, such as object persistency. PyLCGDict is a Python extension module that uses LCG dictionaries, as PyROOT uses CINT reflection information, to allow /cwPython users to access C++ libraries with essentially no preparation on the users' behalf. In addition, and in a similar way, PyROOT gives ROOT users access to Python libraries

  7. Implications of resistance exercise practice to flexibility

    Directory of Open Access Journals (Sweden)

    Cristiane Eri Shiromoto

    2008-06-01

    Full Text Available The aim of this paper is to compare the degrees of flexibility in resistance exercise practitioners before and after a period of six months and verify the relationship between the data and the individuals’ life style. A descriptive method was used for a sample of 46 individuals from Action Academia de Maringá – Pr. The data were collected in Sptember / 2000 and March / 2001 through their frequency and evaluation files and also a goniometer to check the ischia extention. Through the ‘t’ test a significant increase in both male and female flexibility was observed. The style of life and profession may interfere in a negative way although the regular and simultaneous practice of resistance and stretching exercises seem to be a predominant factor in developing flexibility and in physical aptitude favourable degrees for health and life quality.

  8. Plasma jet printing for flexible substrates

    Science.gov (United States)

    Gandhiraman, Ram P.; Singh, Eric; Diaz-Cartagena, Diana C.; Nordlund, Dennis; Koehne, Jessica; Meyyappan, M.

    2016-03-01

    Recent interest in flexible electronics and wearable devices has created a demand for fast and highly repeatable printing processes suitable for device manufacturing. Robust printing technology is critical for the integration of sensors and other devices on flexible substrates such as paper and textile. An atmospheric pressure plasma-based printing process has been developed to deposit different types of nanomaterials on flexible substrates. Multiwalled carbon nanotubes were deposited on paper to demonstrate site-selective deposition as well as direct printing without any type of patterning. Plasma-printed nanotubes were compared with non-plasma-printed samples under similar gas flow and other experimental conditions and found to be denser with higher conductivity. The utility of the nanotubes on the paper substrate as a biosensor and chemical sensor was demonstrated by the detection of dopamine, a neurotransmitter, and ammonia, respectively.

  9. Flexible Developing System for Modern Instrument Manufacturing

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The modern instrument manufacturing has many features, such as high technologies, multiple varieties and small quantities. Aiming at those features, an instrumentation flexible developing system (IFDS) is presented. It constitutes a kind of opening, compatible, extensible and upgradeable flexible developing system, and it will offer a kind of technology equipment for the research and development of new modern instrument products. Its flexible mechanism is able to be suited for different and special requirements of many instrument systems, and its system integrated mechanism is able to adapt to common technological features of most instrument systems. It is IFDS that makes it easy to accomplish a quick system integration of instrument products. IFDS solves key technological problems related to modern instruments development, develops multiple systems for modern instrument development and accomplishes rapid development of series of new products.

  10. Flexibility, persistence, and success in sex therapy.

    Science.gov (United States)

    Sewell, H H; Abramowitz, S I

    1979-11-01

    The predictive utility of patient flexibility as a moderator of persistence and benefit from sex therapy was examined. In addition to a direct self-report flexibility scale, 21 couples completed psychometric measures of sexual and marital compatibility before undergoing a modified Masters and Johnson sexual retraining regimen. Following treatment the 15 continuing pairs filled out the same satisfaction inventories and, along with their male and female cotherapists, made posttherapy ratings of change on sex-specific personal and marital criteria. Flexibility proved to be a strikingly effective discriminator of remainers from early terminators and demonstrated promise as a predictor of favorable sex therapy outcome. Results are discussed in terms of the need to identify and prepare rigid individuals who cannot easily accommodate the relatively directive and value-laden features of sexual retraining. PMID:518289

  11. Dynamic Scheduling of Flexible Job Shops

    Institute of Scientific and Technical Information of China (English)

    SHAHID Ikramullah Butt; SUN Hou-fang

    2007-01-01

    Aim of this research is to minimize makespan in the flexible job shop environment by the use of genetic algorithms and scheduling rules.Software is developed using genetic algorithms and scheduling rules based on certain constraints such as non-preemption of jobs,recirculation,set up times,non-breakdown of machines etc.Purpose of the software is to develop a schedule for flexible job shop environment,which is a special case of job shop scheduling problem.Scheduling algorithm used in the software is verified and tested by using MT10 as benchmark problem,presented in the flexible job shop environment at the end.LEKIN(R) software results are also compared with results of the developed software by the use of MT10 benchmark problem to show that the latter is a practical software and can be used successfully at BIT Training Workshop.

  12. Analytical and Numerical Modeling for Flexible Pipes

    Institute of Scientific and Technical Information of China (English)

    WANG Wei; CHEN Geng

    2011-01-01

    The unbonded flexible pipe of eight layers,in which all the layers except the carcass layer are assumed to have isotropic properties,has been analyzed.Specifically,the carcass layer shows the orthotropic characteristics.The effective elastic moduli of the carcass layer have been developed in terms of the influence of deformation to stiffness.With consideration of the effective elastic moduli,the structure can be properly analyzed.Also the relative movements of tendons and relative displacements of wires in helical armour layer have been investigated.A three-dimensional nonlinear finite element model has been presented to predict the response of flexible pipes under axial force and torque.Further,the friction and contact of interlayer have been considered.Comparison between the finite element model and experimental results obtained in literature has been given and discussed,which might provide practical and technical support for the application of unbonded flexible pipes.

  13. Printed electronic on flexible and glass substrates

    Science.gov (United States)

    Futera, Konrad; Jakubowska, Małgorzata; Kozioł, Grażyna

    2010-09-01

    Organic electronics is a platform technology that enables multiple applications based on organic electronics but varied in specifications. Organic electronics is based on the combination of new materials and cost-effective, large area production processes that provide new fields of application. Organic electronic by its size, weight, flexibility and environmental friendliness electronics enables low cost production of numerous electrical components and provides for such promising fields of application as: intelligent packaging, low cost RFID, flexible solar cells, disposable diagnostic devices or games, and printed batteries [1]. The paper presents results of inkjetted electronics elements on flexible and glass substrates. The investigations was target on characterizing shape, surface and geometry of printed structures. Variety of substrates were investigated, within some, low cost, non specialized substrate, design for other purposes than organic electronic.

  14. A residual flexibility approach to multibody dynamics

    Science.gov (United States)

    Blelloch, Paul A.; Antal, Gregory W.

    1993-01-01

    Many complex systems can be modeled as a collection of interacting bodies, where the relative motion of the bodies may be large. The dynamics of such systems are simulated using multibody dynamic formulations. Many of these treat each body as a rigid component, but recently the flexibility of the components has been incorporated. This paper presents a residual flexibility formulation of the multibody dynamics problem. The formulation is very simple and offers great computational efficiency since it treats each body as a free structure in space, interacting with other bodies only through interface forces. Each body's accelerations can be solved independently, as can each set of interface forces. We have applied the technique successfully to several special applications, and the initial implementation in a general mechanisms code has given excellent results in comparison to a direct finite element representation of flexibility.

  15. A flexible infrared sensor for tissue oximetry

    DEFF Research Database (Denmark)

    Petersen, Søren Dahl; Thyssen, Anders; Engholm, Mathias;

    2013-01-01

    We present a flexible infrared sensor for use in tissue oximetry with the aim of treating prematurely born infants. The sensor will detect the oxygen saturation in brain tissue through near infrared spectroscopy. The sensor itself consists of several individual silicon photo detectors fully...... integrated in a flexible array. The flexibility is achieved by combining silicon with Polydimethylsiloxane and polyimide using standard IC manufacturing. This ensures that the electrical interconnects on the sensor can withstand being bent in order for the sensor to confine to the curved surface of the head...... of a neonatal. The sensor platform has been tested and is found to be very durable and capable of being both bent to small radii of curvature and strained in the longitudinal direction. The electrical interconnects on the sensor only experience a relative small increase in resistance when the sensor is bent up...

  16. Flexible Electronics Development Supported by NASA

    Science.gov (United States)

    Baumann, Eric

    2014-01-01

    The commercial electronics industry is leading development in most areas of electronics for NASA applications; however, working in partnership with industry and the academic community, results from NASA research could lead to better understanding and utilization of electronic materials by the flexible electronics industry. Innovative ideas explored by our partners in industry and the broader U.S. research community help NASA execute our missions and bring new American products and services to the global technology marketplace. [Mike Gazarik, associate administrator for Space Technology, NASA Headquarters, Washington DC] This presentation provides information on NASA needs in electronics looking towards the future, some of the work being supported by NASA in flexible electronics, and the capabilities of the Glenn Research Center supporting the development of flexible electronics.

  17. Recent progress of flexible AMOLED displays

    Science.gov (United States)

    Pang, Huiqing; Rajan, Kamala; Silvernail, Jeff; Mandlik, Prashant; Ma, Ruiqing; Hack, Mike; Brown, Julie J.; Yoo, Juhn S.; Jung, Sang-Hoon; Kim, Yong-Cheol; Byun, Seung-Chan; Kim, Jong-Moo; Yoon, Soo-Young; Kim, Chang-Dong; Hwang, Yong-Kee; Chung, In-Jae; Fletcher, Mark; Green, Derek; Pangle, Mike; McIntyre, Jim; Smith, Randal D.

    2011-03-01

    Significant progress has been made in recent years in flexible AMOLED displays and numerous prototypes have been demonstrated. Replacing rigid glass with flexible substrates and thin-film encapsulation makes displays thinner, lighter, and non-breakable - all attractive features for portable applications. Flexible AMOLEDs equipped with phosphorescent OLEDs are considered one of the best candidates for low-power, rugged, full-color video applications. Recently, we have demonstrated a portable communication display device, built upon a full-color 4.3-inch HVGA foil display with a resolution of 134 dpi using an all-phosphorescent OLED frontplane. The prototype is shaped into a thin and rugged housing that will fit over a user's wrist, providing situational awareness and enabling the wearer to see real-time video and graphics information.

  18. Solution measurement of DNA curvature in papillomavirus E2 binding sites

    OpenAIRE

    Zimmerman, Jeff M.; Maher, L. James

    2003-01-01

    ‘Indirect readout’ refers to the proposal that proteins can recognize the intrinsic three-dimensional shape or flexibility of a DNA binding sequence apart from direct protein contact with DNA base pairs. The differing affinities of human papillomavirus (HPV) E2 proteins for different E2 binding sites have been proposed to reflect indirect readout. DNA bending has been observed in X-ray structures of E2 protein–DNA complexes. X-ray structures of three different E2 DNA binding sites revealed di...

  19. Using remote substituents to control solution structure and anion binding in lanthanide complexes

    DEFF Research Database (Denmark)

    Tropiano, Manuel; Blackburn, Octavia A.; Tilney, James A.;

    2013-01-01

    of the molecule, at a substantial distance from the binding pocket. Herein, we explore these remote substituent effects and explain the observed behaviour through discussion of the way in which remote substituents can influence and control the global structure of a molecule through their demands upon...... conformational space. Peripheral modifications to a binuclear lanthanide motif derived from α,α'-bis(DO3 Ayl)-m-xylene are shown to result in dramatic changes to the binding constant for isophthalate. In this system, the parent compound displays considerable conformational flexibility, yet can be assumed to bind...

  20. Stochastic Dynamics Underlying Cognitive Stability and Flexibility.

    Directory of Open Access Journals (Sweden)

    Kai Ueltzhöffer

    2015-06-01

    Full Text Available Cognitive stability and flexibility are core functions in the successful pursuit of behavioral goals. While there is evidence for a common frontoparietal network underlying both functions and for a key role of dopamine in the modulation of flexible versus stable behavior, the exact neurocomputational mechanisms underlying those executive functions and their adaptation to environmental demands are still unclear. In this work we study the neurocomputational mechanisms underlying cue based task switching (flexibility and distractor inhibition (stability in a paradigm specifically designed to probe both functions. We develop a physiologically plausible, explicit model of neural networks that maintain the currently active task rule in working memory and implement the decision process. We simplify the four-choice decision network to a nonlinear drift-diffusion process that we canonically derive from a generic winner-take-all network model. By fitting our model to the behavioral data of individual subjects, we can reproduce their full behavior in terms of decisions and reaction time distributions in baseline as well as distractor inhibition and switch conditions. Furthermore, we predict the individual hemodynamic response timecourse of the rule-representing network and localize it to a frontoparietal network including the inferior frontal junction area and the intraparietal sulcus, using functional magnetic resonance imaging. This refines the understanding of task-switch-related frontoparietal brain activity as reflecting attractor-like working memory representations of task rules. Finally, we estimate the subject-specific stability of the rule-representing attractor states in terms of the minimal action associated with a transition between different rule states in the phase-space of the fitted models. This stability measure correlates with switching-specific thalamocorticostriatal activation, i.e., with a system associated with flexible working memory

  1. Movable MEMS Devices on Flexible Silicon

    KAUST Repository

    Ahmed, Sally

    2013-05-05

    Flexible electronics have gained great attention recently. Applications such as flexible displays, artificial skin and health monitoring devices are a few examples of this technology. Looking closely at the components of these devices, although MEMS actuators and sensors can play critical role to extend the application areas of flexible electronics, fabricating movable MEMS devices on flexible substrates is highly challenging. Therefore, this thesis reports a process for fabricating free standing and movable MEMS devices on flexible silicon substrates; MEMS flexure thermal actuators have been fabricated to illustrate the viability of the process. Flexure thermal actuators consist of two arms: a thin hot arm and a wide cold arm separated by a small air gap; the arms are anchored to the substrate from one end and connected to each other from the other end. The actuator design has been modified by adding etch holes in the anchors to suit the process of releasing a thin layer of silicon from the bulk silicon substrate. Selecting materials that are compatible with the release process was challenging. Moreover, difficulties were faced in the fabrication process development; for example, the structural layer of the devices was partially etched during silicon release although it was protected by aluminum oxide which is not attacked by the releasing gas . Furthermore, the thin arm of the thermal actuator was thinned during the fabrication process but optimizing the patterning and etching steps of the structural layer successfully solved this problem. Simulation was carried out to compare the performance of the original and the modified designs for the thermal actuators and to study stress and temperature distribution across a device. A fabricated thermal actuator with a 250 μm long hot arm and a 225 μm long cold arm separated by a 3 μm gap produced a deflection of 3 μm before silicon release, however, the fabrication process must be optimized to obtain fully functioning

  2. Stochastic Dynamics Underlying Cognitive Stability and Flexibility.

    Science.gov (United States)

    Ueltzhöffer, Kai; Armbruster-Genç, Diana J N; Fiebach, Christian J

    2015-06-01

    Cognitive stability and flexibility are core functions in the successful pursuit of behavioral goals. While there is evidence for a common frontoparietal network underlying both functions and for a key role of dopamine in the modulation of flexible versus stable behavior, the exact neurocomputational mechanisms underlying those executive functions and their adaptation to environmental demands are still unclear. In this work we study the neurocomputational mechanisms underlying cue based task switching (flexibility) and distractor inhibition (stability) in a paradigm specifically designed to probe both functions. We develop a physiologically plausible, explicit model of neural networks that maintain the currently active task rule in working memory and implement the decision process. We simplify the four-choice decision network to a nonlinear drift-diffusion process that we canonically derive from a generic winner-take-all network model. By fitting our model to the behavioral data of individual subjects, we can reproduce their full behavior in terms of decisions and reaction time distributions in baseline as well as distractor inhibition and switch conditions. Furthermore, we predict the individual hemodynamic response timecourse of the rule-representing network and localize it to a frontoparietal network including the inferior frontal junction area and the intraparietal sulcus, using functional magnetic resonance imaging. This refines the understanding of task-switch-related frontoparietal brain activity as reflecting attractor-like working memory representations of task rules. Finally, we estimate the subject-specific stability of the rule-representing attractor states in terms of the minimal action associated with a transition between different rule states in the phase-space of the fitted models. This stability measure correlates with switching-specific thalamocorticostriatal activation, i.e., with a system associated with flexible working memory updating and

  3. Research on Risk Management of Flexibility Information Systems

    OpenAIRE

    Liangqiang LI; Li, Dong; Peiji SHAO; Yang, Feng

    2011-01-01

    This paper applies a document reading method and deductive-inductive method, studies idea of flexibility MIS, and Risk Management of Flexibility Information Systems on its development and operating environment, discusses an effect on information system risk administration brought by flexibility MIS idea, suggests that the information system risk management framework and implementing strategy by flexibility idea.
    Key words: Flexibility MIS; Information...

  4. Effects of defeat stress on behavioral flexibility in males and females: modulation by the mu-opioid receptor

    Science.gov (United States)

    Laredo, Sarah A.; Steinman, Michael Q.; Robles, Cindee F.; Ferrer, Emilio; Ragen, Benjamin J.; Trainor, Brian C.

    2014-01-01

    Behavioral flexibility is a component of executive functioning that allows individuals to adapt to changing environmental conditions. Independent lines of research indicate that the mu opioid receptor (MOR) is an important mediator of behavioral flexibility and responses to psychosocial stress. The current study bridges these two lines of research and tests the extent to which social defeat and MOR affect behavioral flexibility and whether sex moderates these effects in California mice (Peromyscus californicus). Males and females assigned to social defeat or control conditions were tested in a Barnes maze. In males, defeat impaired behavioral flexibility but not acquisition. Female performance was unaffected by defeat. MOR binding in defeated and control mice in the orbitofrontal cortex (OFC), striatum, and hippocampus was examined via autoradiography. Stressed males had reduced MOR binding in the OFC whereas females were unaffected. The MOR antagonist beta-funaltrexamine (1 mg/kg) impaired performance in males naïve to defeat during the reversal phase but had no effect on females. Finally, we examined the effects of the MOR agonist morphine (2.5, 5 mg/kg) on stressed mice. As expected, morphine improved behavioral flexibility in stressed males. The stress-induced deficits in behavioral flexibility in males are consistent with a proactive coping strategy, including previous observations that stressed male California mice exhibit strong social approach and aggression. Our pharmacological data suggest that a down-regulation of MOR signaling in males may contribute to sex differences in behavioral flexibility following stress. This is discussed in the framework of coping strategies for individuals with mood disorders. PMID:25615538

  5. Rigid-flexible printed wiring boards

    Science.gov (United States)

    Sydow, E. V.

    1993-05-01

    Rigid-flexible printed wiring board materials, fabrication processes, product requirements, and reliability were established. Considerable effort was spent testing plated-through holes, analyzing plated-through hole failure mechanisms, and then incorporating materials, processes, and product requirements to provide adequate control and reduce failures. The principal processes developed were tooling concepts, process sequence, lamination, drilling, plasma etchback, copper electroplating, and solder coating. Product requirements and reliability were established with thermal shock, thermal stress, innerlayer adhesion, and fold flexibility tests. Plated-through hole failure mechanisms were analyzed with thermal mechanical and finite element analyses.

  6. Flexible input, dazzling output with IBM i

    CERN Document Server

    Victória-Pereira, Rafael

    2014-01-01

    Link your IBM i system to the modern business server world! This book presents easier and more flexible ways to get data into your IBM i system, along with rather surprising methods to export and present the vital business data it contains. You'll learn how to automate file transfers, seamlessly connect PC applications with your RPG programs, and much more. Input operations will become more flexible and user-proof, with self-correcting import processes and direct file transfers that require a minimum of user intervention. Also learn novel ways to present information: your DB2 data will look gr

  7. Innovation in wearable and flexible antennas

    CERN Document Server

    Khaleel, Haider

    2014-01-01

    This book deals with the design, numerical simulation, state of the art fabrication processes and methods, qualitative and quantitative tests, and measurement techniques of wearable and flexible antennas of different topologies, such as: Planar Inverted F, Printed Monopoles, Micropoles and Microstrips. Novel trends, materials, and fabrication and measurement techniques used in this vital field of antenna systems are also discussed.To the best of the editor's knowledge, at the time of publication, there are no published books targeting the vital topic of flexible antennas specifically and/or se

  8. Gigahertz frequency flexible carbon nanotube transistors

    Science.gov (United States)

    Chimot, N.; Derycke, V.; Goffman, M. F.; Bourgoin, J. P.; Happy, H.; Dambrine, G.

    2007-10-01

    We investigate the high frequency performances of flexible field-effect transistors based on carbon nanotubes. A large density of mostly aligned carbon nanotubes deposited on a flexible substrate by dielectrophoresis serves as the channel. The transistors display a constant transconductance up to at least 6GHz and a current gain cutoff frequency (fT) as high as 1GHz at VDS=-700mV. Bending tests show that the devices can withstand a high degree of flexion characterized by a constant transconductance for radius of curvature as small as 3.3mm.

  9. ANFIS Modelling of Flexible Plate Structure

    Directory of Open Access Journals (Sweden)

    A. A. M. Al-Khafaji

    2010-06-01

    Full Text Available This paper presented an investigation into the performance of system identification using an Adaptive Neuro-Fuzzy Inference System (ANFIS technique for the dynamic modelling of a twodimensional flexible plate structure. It is confirmed experimentally, using National Instrumentation (NI Data Acquisition System (DAQ and flexible plate test rig that ANFIS can be effectively used for modelling the system with highly accurate results. The accuracy of the modelling results is demonstrated through validation tests including training and test validation and correlation tests.

  10. Flexible Polyhedral Surfaces with Two Flat Poses

    Directory of Open Access Journals (Sweden)

    Hellmuth Stachel

    2015-05-01

    Full Text Available We present three types of polyhedral surfaces, which are continuously flexible and have not only an initial pose, where all faces are coplanar, but pass during their self-motion through another pose with coplanar faces (“flat pose”. These surfaces are examples of so-called rigid origami, since we only admit exact flexions, i.e., each face remains rigid during the motion; only the dihedral angles vary. We analyze the geometry behind Miura-ori and address Kokotsakis’ example of a flexible tessellation with the particular case of a cyclic quadrangle. Finally, we recall Bricard’s octahedra of Type 3 and their relation to strophoids.

  11. Flexible robotics applications to multiscale manipulations

    CERN Document Server

    Grossard , Mathieu; Regnier, Stephane

    2013-01-01

    The objective of this book is to provide those interested in the field of flexible robotics with an overview of several scientific and technological advances in the practical field of robotic manipulation. The different chapters examine various stages that involve a number of robotic devices, particularly those designed for manipulation tasks characterized by mechanical flexibility. Chapter 1 deals with the general context surrounding the design of functionally integrated microgripping systems. Chapter 2 focuses on the dual notations of modal commandability and observability, which play a sig

  12. New binding site conformations of the dengue virus NS3 protease accessed by molecular dynamics simulation.

    Directory of Open Access Journals (Sweden)

    Hugo de Almeida

    Full Text Available Dengue fever is caused by four distinct serotypes of the dengue virus (DENV1-4, and is estimated to affect over 500 million people every year. Presently, there are no vaccines or antiviral treatments for this disease. Among the possible targets to fight dengue fever is the viral NS3 protease (NS3PRO, which is in part responsible for viral processing and replication. It is now widely recognized that virtual screening campaigns should consider the flexibility of target protein by using multiple active conformational states. The flexibility of the DENV NS3PRO could explain the relatively low success of previous virtual screening studies. In this first work, we explore the DENV NS3PRO conformational states obtained from molecular dynamics (MD simulations to take into account protease flexibility during the virtual screening/docking process. To do so, we built a full NS3PRO model by multiple template homology modeling. The model comprised the NS2B cofactor (essential to the NS3PRO activation, a glycine flexible link and the proteolytic domain. MD simulations had the purpose to sample, as closely as possible, the ligand binding site conformational landscape prior to inhibitor binding. The obtained conformational MD sample was clustered into four families that, together with principal component analysis of the trajectory, demonstrated protein flexibility. These results allowed the description of multiple binding modes for the Bz-Nle-Lys-Arg-Arg-H inhibitor, as verified by binding plots and pair interaction analysis. This study allowed us to tackle protein flexibility in our virtual screening campaign against the dengue virus NS3 protease.

  13. SCOWLP classification: Structural comparison and analysis of protein binding regions

    Directory of Open Access Journals (Sweden)

    Anders Gerd

    2008-01-01

    Full Text Available Abstract Background Detailed information about protein interactions is critical for our understanding of the principles governing protein recognition mechanisms. The structures of many proteins have been experimentally determined in complex with different ligands bound either in the same or different binding regions. Thus, the structural interactome requires the development of tools to classify protein binding regions. A proper classification may provide a general view of the regions that a protein uses to bind others and also facilitate a detailed comparative analysis of the interacting information for specific protein binding regions at atomic level. Such classification might be of potential use for deciphering protein interaction networks, understanding protein function, rational engineering and design. Description Protein binding regions (PBRs might be ideally described as well-defined separated regions that share no interacting residues one another. However, PBRs are often irregular, discontinuous and can share a wide range of interacting residues among them. The criteria to define an individual binding region can be often arbitrary and may differ from other binding regions within a protein family. Therefore, the rational behind protein interface classification should aim to fulfil the requirements of the analysis to be performed. We extract detailed interaction information of protein domains, peptides and interfacial solvent from the SCOWLP database and we classify the PBRs of each domain family. For this purpose, we define a similarity index based on the overlapping of interacting residues mapped in pair-wise structural alignments. We perform our classification with agglomerative hierarchical clustering using the complete-linkage method. Our classification is calculated at different similarity cut-offs to allow flexibility in the analysis of PBRs, feature especially interesting for those protein families with conflictive binding regions

  14. Consistent improvement of cross-docking results using binding site ensembles generated with elastic network normal modes.

    Science.gov (United States)

    Rueda, Manuel; Bottegoni, Giovanni; Abagyan, Ruben

    2009-03-01

    The representation of protein flexibility is still a challenge for the state-of-the-art flexible ligand docking protocols. In this article, we use a large and diverse benchmark to prove that is possible to improve consistently the cross-docking performance against a single receptor conformation, using an equilibrium ensemble of binding site conformers. The benchmark contained 28 proteins, and our method predicted the top-ranked near native ligand poses 20% more efficiently than using a single receptor. The multiple conformations were derived from the collective variable space defined by all heavy-atom elastic network normal modes, including backbone and side chains. We have found that the binding site displacements for best positioning of the ligand seem rather independent from the global collective motions of the protein. We also found that the number of binding site conformations needed to represent nonredundant flexibility was Web site at http://abagyan.scripps.edu/MRC. PMID:19434904

  15. Free-energy-based methods for binding profile determination in a congeneric series of CDK2 inhibitors.

    Science.gov (United States)

    Fidelak, Jérémy; Juraszek, Jarek; Branduardi, Davide; Bianciotto, Marc; Gervasio, Francesco Luigi

    2010-07-29

    Free-energy pathway methods show great promise in computing the mode of action and the free energy profile associated with the binding of small molecules with proteins, but are generally very computationally demanding. Here we apply a novel approach based on metadynamics and path collective variables. We show that this combination is able to find an optimal reaction coordinate and the free energy profile of binding with explicit solvent and full flexibility, while minimizing human intervention and computational costs. We apply it to predict the binding affinity of a congeneric series of 5 CDK2 inhibitors. The predicted binding free energy profiles are in accordance with experiment. PMID:20593892

  16. DNA-binding residues and binding mode prediction with binding-mechanism concerned models

    OpenAIRE

    Oyang Yen-Jen; Liu Yu-Cheng; Huang Chun-Chin; Huang Yu-Feng; Huang Chien-Kang

    2009-01-01

    Abstract Background Protein-DNA interactions are essential for fundamental biological activities including DNA transcription, replication, packaging, repair and rearrangement. Proteins interacting with DNA can be classified into two categories of binding mechanisms - sequence-specific and non-specific binding. Protein-DNA specific binding provides a mechanism to recognize correct nucleotide base pairs for sequence-specific identification. Protein-DNA non-specific binding shows sequence indepe...

  17. An in silico analysis of the binding modes and binding affinities of small molecule modulators of PDZ-peptide interactions.

    Directory of Open Access Journals (Sweden)

    Garima Tiwari

    Full Text Available Inhibitors of PDZ-peptide interactions have important implications in a variety of biological processes including treatment of cancer and Parkinson's disease. Even though experimental studies have reported characterization of peptidomimetic inhibitors of PDZ-peptide interactions, the binding modes for most of them have not been characterized by structural studies. In this study we have attempted to understand the structural basis of the small molecule-PDZ interactions by in silico analysis of the binding modes and binding affinities of a set of 38 small molecules with known K(i or K(d values for PDZ2 and PDZ3 domains of PSD-95 protein. These two PDZ domains show differential selectivity for these compounds despite having a high degree of sequence similarity and almost identical peptide binding pockets. Optimum binding modes for these ligands for PDZ2 and PDZ3 domains were identified by using a novel combination of semi-flexible docking and explicit solvent molecular dynamics (MD simulations. Analysis of the binding modes revealed most of the peptidomimectic ligands which had high K(i or K(d moved away from the peptide binding pocket, while ligands with high binding affinities remained in the peptide binding pocket. The differential specificities of the PDZ2 and PDZ3 domains primarily arise from differences in the conformation of the loop connecting βB and βC strands, because this loop interacts with the N-terminal chemical moieties of the ligands. We have also computed the MM/PBSA binding free energy values for these 38 compounds with both the PDZ domains from multiple 5 ns MD trajectories on each complex i.e. a total of 228 MD trajectories of 5 ns length each. Interestingly, computational binding free energies show good agreement with experimental binding free energies with a correlation coefficient of approximately 0.6. Thus our study demonstrates that combined use of docking and MD simulations can help in identification of potent inhibitors

  18. Carboplatin binding to histidine

    International Nuclear Information System (INIS)

    An X-ray crystal structure showing the binding of purely carboplatin to histidine in a model protein has finally been obtained. This required extensive crystallization trials and various novel crystal structure analyses. Carboplatin is a second-generation platinum anticancer agent used for the treatment of a variety of cancers. Previous X-ray crystallographic studies of carboplatin binding to histidine (in hen egg-white lysozyme; HEWL) showed the partial conversion of carboplatin to cisplatin owing to the high NaCl concentration used in the crystallization conditions. HEWL co-crystallizations with carboplatin in NaBr conditions have now been carried out to confirm whether carboplatin converts to the bromine form and whether this takes place in a similar way to the partial conversion of carboplatin to cisplatin observed previously in NaCl conditions. Here, it is reported that a partial chemical transformation takes place but to a transplatin form. Thus, to attempt to resolve purely carboplatin binding at histidine, this study utilized co-crystallization of HEWL with carboplatin without NaCl to eliminate the partial chemical conversion of carboplatin. Tetragonal HEWL crystals co-crystallized with carboplatin were successfully obtained in four different conditions, each at a different pH value. The structural results obtained show carboplatin bound to either one or both of the N atoms of His15 of HEWL, and this particular variation was dependent on the concentration of anions in the crystallization mixture and the elapsed time, as well as the pH used. The structural details of the bound carboplatin molecule also differed between them. Overall, the most detailed crystal structure showed the majority of the carboplatin atoms bound to the platinum centre; however, the four-carbon ring structure of the cyclobutanedicarboxylate moiety (CBDC) remained elusive. The potential impact of the results for the administration of carboplatin as an anticancer agent are described

  19. Carboplatin binding to histidine

    Energy Technology Data Exchange (ETDEWEB)

    Tanley, Simon W. M. [University of Manchester, Brunswick Street, Manchester M13 9PL (United Kingdom); Diederichs, Kay [University of Konstanz, D-78457 Konstanz (Germany); Kroon-Batenburg, Loes M. J. [Utrecht University, Padualaan 8, 3584 CH Utrecht (Netherlands); Levy, Colin [University of Manchester, 131 Princess Street, Manchester M1 7DN (United Kingdom); Schreurs, Antoine M. M. [Utrecht University, Padualaan 8, 3584 CH Utrecht (Netherlands); Helliwell, John R., E-mail: john.helliwell@manchester.ac.uk [University of Manchester, Brunswick Street, Manchester M13 9PL (United Kingdom)

    2014-08-29

    An X-ray crystal structure showing the binding of purely carboplatin to histidine in a model protein has finally been obtained. This required extensive crystallization trials and various novel crystal structure analyses. Carboplatin is a second-generation platinum anticancer agent used for the treatment of a variety of cancers. Previous X-ray crystallographic studies of carboplatin binding to histidine (in hen egg-white lysozyme; HEWL) showed the partial conversion of carboplatin to cisplatin owing to the high NaCl concentration used in the crystallization conditions. HEWL co-crystallizations with carboplatin in NaBr conditions have now been carried out to confirm whether carboplatin converts to the bromine form and whether this takes place in a similar way to the partial conversion of carboplatin to cisplatin observed previously in NaCl conditions. Here, it is reported that a partial chemical transformation takes place but to a transplatin form. Thus, to attempt to resolve purely carboplatin binding at histidine, this study utilized co-crystallization of HEWL with carboplatin without NaCl to eliminate the partial chemical conversion of carboplatin. Tetragonal HEWL crystals co-crystallized with carboplatin were successfully obtained in four different conditions, each at a different pH value. The structural results obtained show carboplatin bound to either one or both of the N atoms of His15 of HEWL, and this particular variation was dependent on the concentration of anions in the crystallization mixture and the elapsed time, as well as the pH used. The structural details of the bound carboplatin molecule also differed between them. Overall, the most detailed crystal structure showed the majority of the carboplatin atoms bound to the platinum centre; however, the four-carbon ring structure of the cyclobutanedicarboxylate moiety (CBDC) remained elusive. The potential impact of the results for the administration of carboplatin as an anticancer agent are described.

  20. Viscous pumping inspired by flexible propulsion

    International Nuclear Information System (INIS)

    Fluid-suspended microorganisms have evolved different swimming and feeding strategies in order to cope with an environment dominated by viscous effects. For instance, ciliated organisms rely on the collective motion of flexible appendages to move and feed. By performing a non-reciprocal motion, flexible filaments can produce a net propulsive force, or pump fluid, in the absence of inertia. Inspired by such a fundamental concept, we propose a strategy to produce macroscopic pumping and mixing in creeping flow. We measured experimentally the net motion of a Newtonian viscous fluid induced by the reciprocal motion of a flapper. When the flapper is rigid no net motion is induced. In contrast, when the flapper is made of a flexible material, a net fluid pumping is measured. We quantify the effectiveness of this pumping strategy and show that optimal pumping is achieved when the length of the flapper is on the same order as the elasto-hydrodynamic penetration length. We finally discuss the possible applications of flexible impellers in mixing operations at low Reynolds numbers. (paper)

  1. Flexible spin-orbit torque devices

    International Nuclear Information System (INIS)

    We report on state-of-the-art spintronic devices synthesized and fabricated directly on a flexible organic substrate. Large perpendicular magnetic anisotropy was achieved in ultrathin ferromagnetic heterostructures of Pt/Co/MgO sputtered on a non-rigid plastic substrate at room temperature. Subsequently, a full magnetic reversal of the Co was observed by exploiting the spin orbit coupling in Pt that leads to a spin accumulation at the Pt/Co interface when an in-plane current is applied. Quasi-static measurements show the potential for operating these devices at nano-second speeds. Importantly, the behavior of the devices remained unchanged under varying bending conditions (up to a bending radius of ≈ ±20–30 mm). Furthermore, the devices showed robust operation even after application of 106 successive pulses, which is likely sufficient for many flexible applications. Thus, this work demonstrates the potential for integrating high performance spintronic devices on flexible substrates, which could lead to many applications ranging from flexible non-volatile magnetic memory to local magnetic resonance imaging

  2. Prevention of pulsations caused by flexible risers

    NARCIS (Netherlands)

    Belfroid S.P.C; Golliard, J.; Korst, H.J.C.

    2013-01-01

    In the last few decades, flexible risers have increasingly been used in the offshore oil and gas industry. In gas applications these risers can generate high amplitude tonal pressure fluctuations when the gas velocity reaches a threshold value. The resulting pressure fluctuations can then cause high

  3. Modeling protein flexibility by distance geometry

    OpenAIRE

    Le Boudic-Jamin, Mathilde; Mucherino, Antonio; Andonov, Rumen

    2012-01-01

    National audience This long abstract discusses a strategy for modeling protein flexibility which is based on the discretization of the space of possible molecular conformations for a protein. The same discretization process was previously employed for discretizing Molecular Distance Geometry Problems (MDGPs).

  4. Unlock the Rigidity and Design for Flexibility

    Science.gov (United States)

    Spante, Maria; Ericsson, Thommy; Sunnerstam, Maria; Huang-DeVoss, Cammy; Axelsson, Michael

    2010-01-01

    This paper reports findings from a project implementing the VL (virtual labs) site featuring flash-based animations developed at Stanford University. The main conclusion in this paper stresses the need to design for flexibility and adaptability of interactive media to better suit the specific situation teachers encounter in their everyday work, in…

  5. Flexible, Mastery-Oriented Astrophysics Sequence.

    Science.gov (United States)

    Zeilik, Michael, II

    1981-01-01

    Describes the implementation and impact of a two-semester mastery-oriented astrophysics sequence for upper-level physics/astrophysics majors designed to handle flexibly a wide range of student backgrounds. A Personalized System of Instruction (PSI) format was used fostering frequent student-instructor interaction and role-modeling behavior in…

  6. Flexible frontiers for text division into rows

    Directory of Open Access Journals (Sweden)

    Dan L. Lacrămă

    2009-01-01

    Full Text Available This paper presents an original solution for flexible hand-written text division into rows. Unlike the standard procedure, the proposed method avoids the isolated characters extensions amputation and reduces the recognition error rate in the final stage.

  7. 78 FR 1698 - Semiannual Regulatory Flexibility Agenda

    Science.gov (United States)

    2013-01-08

    ... to facilitate the banking industry's ongoing transition to fully electronic interbank check... whether a deposited check has been returned unpaid. Timetable: Action Date FR Cite Board Requested Comment 03/25/11 76 FR 16862 Board Expects Further Action........ 12/00/12 Regulatory Flexibility...

  8. Enabling flexible processes by ECA orchestration architecture

    NARCIS (Netherlands)

    Gong, Y.; Janssen, M.; Overbeek, S.J.; Zuurmond, A.

    2009-01-01

    Governments are looking for ways to change their business processes rapidly to adapt to the ever-changing legislation, customer needs, and other changes in the environment. In recent years, the need for more flexibility has become eminent due to the long implementation times of new legislations. As

  9. Workplace Flexibility: From Research to Action

    Science.gov (United States)

    Galinsky, Ellen; Sakai, Kelly; Wigton, Tyler

    2011-01-01

    Ellen Galinsky, Kelly Sakai, and Tyler Wigton explore the "time famine" among American workers--the continuing sense among employees of not having enough time to manage the multiple responsibilities of work and personal and family life. Noting that large shares of U.S. employees report feeling the need for greater workplace flexibility to enable…

  10. Locomotion of a flapping flexible plate

    Science.gov (United States)

    Hua, Ru-Nan; Zhu, Luoding; Lu, Xi-Yun

    2013-12-01

    The locomotion of a flapping flexible plate in a viscous incompressible stationary fluid is numerically studied by an immersed boundary-lattice Boltzmann method for the fluid and a finite element method for the plate. When the leading-edge of the flexible plate is forced to heave sinusoidally, the entire plate starts to move freely as a result of the fluid-structure interaction. Mechanisms underlying the dynamics of the plate are elucidated. Three distinct states of the plate motion are identified and can be described as forward, backward, and irregular. Which state to occur depends mainly on the heaving amplitude and the bending rigidity of the plate. In the forward motion regime, analysis of the dynamic behaviors of the flapping flexible plate indicates that a suitable degree of flexibility can improve the propulsive performance. Moreover, there exist two kinds of vortex streets in the downstream of the plate which are normal and deflected wake. Further the forward motion is compared with the flapping-based locomotion of swimming and flying animals. The results obtained in the present study are found to be consistent with the relevant observations and measurements and can provide some physical insights into the understanding of the propulsive mechanisms of swimming and flying animals.

  11. Flexible Scheduling to Fit the Firefighters.

    Science.gov (United States)

    Cox, Clarice Robinson

    Three flexible scheduling plans were tried in order that firefighters could take regular college courses despite their 24 hours on the 24 off work schedule. Plan one scheduled the firefighters into a regular Monday-Wednesday-Friday class which they attended every other week, making up missed material outside of class. Plan two scheduled special…

  12. Computer program for flexible rotor dynamics analysis

    Science.gov (United States)

    Shen, F. A.

    1974-01-01

    Program analyzes general nonaxisymmetric and nonsynchronous transient and steady-state rotor dynamic performance of bending- and shear-wise flexible rotor-bearing system under various operating conditions. Program can be used as analytical study tool for general transient spin-speed and/or non-axisymmetric rotor motion.

  13. Labour Market Flexibility: Trends in Enterprises.

    Science.gov (United States)

    Organisation for Economic Cooperation and Development, Paris (France).

    Since the mid-1980s, the Organisation for Economic Cooperation and Development (OECD) has carried out a number of studies on the issues of labor market flexibility under the supervision of the Manpower and Social Affairs Committee and its Working Party on Industrial Relations. While much of the previous work focused on the external forms of labor…

  14. Flexible Twin Open Ring Metro WDM Network

    Institute of Scientific and Technical Information of China (English)

    Susumu Kinoshita; Cechan Tian; Satoru Ohdate; Koji Takeguchi; Yasuhiko Aoki

    2003-01-01

    We have demonstrated a flexible twin open ring WDM network for metro applications. A pair of optical switches in the network keeps the fiber rings open to prevent signal circulation. Traffics are broadcast to every node and selected at the receiving side. Superior transmission and protection switching are proved.

  15. Flexible Hybrid Organic-Inorganic Perovskite Memory.

    Science.gov (United States)

    Gu, Chungwan; Lee, Jang-Sik

    2016-05-24

    Active research has been done on hybrid organic-inorganic perovskite materials for application to solar cells with high power conversion efficiency. However, this material often shows hysteresis, which is undesirable, shift in the current-voltage curve. The hysteresis may come from formation of defects and their movement in perovskite materials. Here, we utilize the defects in perovskite materials to be used in memory operations. We demonstrate flexible nonvolatile memory devices based on hybrid organic-inorganic perovskite as the resistive switching layer on a plastic substrate. A uniform perovskite layer is formed on a transparent electrode-coated plastic substrate by solvent engineering. Flexible nonvolatile memory based on the perovskite layer shows reproducible and reliable memory characteristics in terms of program/erase operations, data retention, and endurance properties. The memory devices also show good mechanical flexibility. It is suggested that resistive switching is done by migration of vacancy defects and formation of conducting filaments under the electric field in the perovskite layer. It is believed that organic-inorganic perovskite materials have great potential to be used in high-performance, flexible memory devices. PMID:27093096

  16. Flexibility through Online Learning. At a Glance.

    Science.gov (United States)

    National Centre for Vocational Education Research, Leabrook (Australia).

    Research funded by the Flexible Learning Advisory Group during 2000-2002 on issues related to online learning and delivery in vocational education and training in Australia was consolidated. Key findings included the following: (1) most students who experienced online learning did so in a conventional class, using face-to-face and other strategies…

  17. FAME - A Flexible Appearance Modelling Environment

    DEFF Research Database (Denmark)

    Stegmann, Mikkel Bille; Ersbøll, Bjarne Kjær; Larsen, Rasmus

    2003-01-01

    applications within medicine and describes a public domain implementation, namely the Flexible Appearance Modelling Environment (FAME). We give guidelines for the use of this research platform, and show that the optimisation techniques used renders it applicable to interactive medical applications. To increase...

  18. Flexible shielding system for radiation protection

    Science.gov (United States)

    Babin, A.

    1972-01-01

    Modular construction of low cost flexible radiation shielding panels consists of water filled steels cans, zinc bromide windows, turntable unit, master-slave manipulators, and interlocking lead bricks. Easy modifications of shielding wall thicknesses are obtained by rearranging overall geometry of portable components.

  19. Framework for Flexible Security in Group Communications

    Science.gov (United States)

    McDaniel, Patrick; Prakash, Atul

    2006-01-01

    The Antigone software system defines a framework for the flexible definition and implementation of security policies in group communication systems. Antigone does not dictate the available security policies, but provides high-level mechanisms for implementing them. A central element of the Antigone architecture is a suite of such mechanisms comprising micro-protocols that provide the basic services needed by secure groups.

  20. Flexible hydrogel-based functional composite materials

    Science.gov (United States)

    Song, Jie; Saiz, Eduardo; Bertozzi, Carolyn R; Tomasia, Antoni P

    2013-10-08

    A composite having a flexible hydrogel polymer formed by mixing an organic phase with an inorganic composition, the organic phase selected from the group consisting of a hydrogel monomer, a crosslinker, a radical initiator, and/or a solvent. A polymerization mixture is formed and polymerized into a desired shape and size.

  1. Simulation of Flexible Objects in Robotics

    DEFF Research Database (Denmark)

    Fugl, Andreas Rune; Petersen, Henrik Gordon; Willatzen, Morten

    2012-01-01

    In this paper, we present what appears to be the first simulation model for grasping of flexible bodies based on the three-dimensional elastic constitutive relations and Newton's Second Law for solids known as the Navier-Cauchy equations. We give an overview of the most important equations for st...

  2. Flexible spin-orbit torque devices

    Energy Technology Data Exchange (ETDEWEB)

    Lee, OukJae; You, Long; Jang, Jaewon; Subramanian, Vivek [Department of Electrical Engineering and Computer Sciences, University of California at Berkeley, Berkeley, California 94720 (United States); Salahuddin, Sayeef [Department of Electrical Engineering and Computer Sciences, University of California at Berkeley, Berkeley, California 94720 (United States); Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (United States)

    2015-12-21

    We report on state-of-the-art spintronic devices synthesized and fabricated directly on a flexible organic substrate. Large perpendicular magnetic anisotropy was achieved in ultrathin ferromagnetic heterostructures of Pt/Co/MgO sputtered on a non-rigid plastic substrate at room temperature. Subsequently, a full magnetic reversal of the Co was observed by exploiting the spin orbit coupling in Pt that leads to a spin accumulation at the Pt/Co interface when an in-plane current is applied. Quasi-static measurements show the potential for operating these devices at nano-second speeds. Importantly, the behavior of the devices remained unchanged under varying bending conditions (up to a bending radius of ≈ ±20–30 mm). Furthermore, the devices showed robust operation even after application of 10{sup 6} successive pulses, which is likely sufficient for many flexible applications. Thus, this work demonstrates the potential for integrating high performance spintronic devices on flexible substrates, which could lead to many applications ranging from flexible non-volatile magnetic memory to local magnetic resonance imaging.

  3. Collagen binding to Staphylococcus aureus

    International Nuclear Information System (INIS)

    Staphylococcus aureus can bind soluble collagen in a specific, saturable manner. We have previously shown that some variability exists in the degree of collagen binding between different strains of heat-killed, formaldehyde-fixed S. aureus which are commercially available as immunologic reagents. The present study demonstrates that live S. aureus of the Cowan 1 strain binds amounts of collagen per organism equivalent to those demonstrated previously in heat-killed, formaldehyde-fixed bacteria but has an affinity over 100 times greater, with Kd values of 9.7 X 10(-11) M and 4.3 X 10(-8) M for live and heat-killed organisms, respectively. Studies were also carried out with S. aureus killed by ionizing radiation, since this method of killing the organism seemed less likely to alter the binding moieties on the surface than did heat killing. Bacteria killed by exposure to gamma radiation bound collagen in a manner essentially indistinguishable from that of live organisms. Binding of collagen to irradiated cells of the Cowan 1 strain was rapid, with equilibrium reached by 30 min at 22 degrees C, and was fully reversible. The binding was not inhibited by fibronectin, fibrinogen, C1q, or immunoglobulin G, suggesting a binding site for collagen distinct from those for these proteins. Collagen binding was virtually eliminated in trypsin-treated organisms, indicating that the binding site has a protein component. Of four strains examined, Cowan 1 and S. aureus ATCC 25923 showed saturable, specific binding, while strains Woods and S4 showed a complete lack of binding. These results suggest that some strains of S. aureus contain high-affinity binding sites for collagen. While the number of binding sites per bacterium varied sixfold in the two collagen-binding strains, the apparent affinity was similar

  4. Mechanistic insights into phosphopeptide--BRCT domain association: preorganization, flexibility, and phosphate recognition.

    Science.gov (United States)

    Huang, Yu-ming M; Kang, Myungshim; Chang, Chia-en A

    2012-08-30

    Promiscuous proteins are commonly observed in biological systems, for example, in modular domains that recognize phosphopeptides during signal transduction. This promiscuous recognition is of fundamental interest in chemistry and biology but is challenging when designing phosphopeptides in silico for cell biology studies. To investigate promiscuous recognition and binding processes of phosphopeptides and the modular domain, we selected a domain essential in breast cancer-the breast-cancer-associated protein 1 (BRCA1) C-terminal (BRCT) repeats as our model system. We performed molecular dynamics simulations and detailed analyses of the dihedral space to study protein fluctuation and conformational changes with phosphopeptide binding. We also studied the association processes of phosphorylated and unphosphorylated peptides using Brownian dynamics with a coarse-grained model. We found that the BRCT domain is preorganized for phosphopeptide binding but has a moderate arrangement of side chains to form complexes with various types of phosphopeptides. Phosphopeptide binding restricts the system motion in general, while the nonpolar phosphopeptide becomes more flexible in the bound state. Our analysis found that the BRCT domain utilizes different mechanisms, usually termed lock and key, induced-fit, and population-shift/conformational-selection models, to recognize peptides with different features. Brownian dynamics simulations revealed that the charged phosphate group may not always accelerate peptide association processes, but it helps the phosphopeptide orient into binding pockets accurately and stabilizes the complex. This work provides insights into molecular recognition in the promiscuous protein system. PMID:22857521

  5. Flexible Structure of Peptide-Bound Filamin A Mechanosensor Domain Pair 20-21.

    Directory of Open Access Journals (Sweden)

    Jonne Seppälä

    Full Text Available Filamins (FLNs are large, multidomain actin cross-linking proteins with diverse functions. Besides regulating the actin cytoskeleton, they serve as important links between the extracellular matrix and the cytoskeleton by binding cell surface receptors, functioning as scaffolds for signaling proteins, and binding several other cytoskeletal proteins that regulate cell adhesion dynamics. Structurally, FLNs are formed of an amino terminal actin-binding domain followed by 24 immunoglobulin-like domains (IgFLNs. Recent studies have demonstrated that myosin-mediated contractile forces can reveal hidden protein binding sites in the domain pairs IgFLNa18-19 and 20-21, enabling FLNs to transduce mechanical signals in cells. The atomic structures of these mechanosensor domain pairs in the resting state are known, as well as the structures of individual IgFLN21 with ligand peptides. However, little experimental data is available on how interacting protein binding deforms the domain pair structures. Here, using small-angle x-ray scattering-based modelling, x-ray crystallography, and NMR, we show that the adaptor protein migfilin-derived peptide-bound structure of IgFLNa20-21 is flexible and adopts distinctive conformations depending on the presence or absence of the interacting peptide. The conformational changes reported here may be common for all peptides and may play a role in the mechanosensor function of the site.

  6. Melanin binding radiopharmaceuticals

    International Nuclear Information System (INIS)

    We have determined the biodistribution an uptake by the Greene melanoma in the Syrian golden hamster with 21 radiopharmaceuticals. Maximum % uptake and the time at which this occurred are listed. It is essential to know maximum tumor to background ration and the time after injection that this occurs to determine suitability for tumor scanning. The importance of species variation deserves mention. Detection of eye melanoma in humans was quite variable whereas in hamsters it was quite easy to obtain a positive scan with a single pinhole. We then looked at brain uptake in man and found it (the brain scan) to be significant. In addition, we found a high uptake by the lung, something not found in hamsters but not entirely unsuspected of a amine, such as 123I-4,3DMQ. Finally, our clinical experience has shown us some of the vagaries of melanoma-seeking radiopharmaceuticals. This reflects the complexity of melanin and melanin-binding and points out the necessity for a more detailed analysis of the mechanisms involved in melanin binding radionuclides

  7. The genome landscape of ER{alpha}- and ER{beta}-binding DNA regions

    DEFF Research Database (Denmark)

    Liu, Yawen; Gao, Hui; Marstrand, Troels Torben;

    2008-01-01

    also regions that are bound by ERalpha only in the presence of ERbeta, as well as regions that are selectively bound by either receptor. Analysis of bound regions shows that regions bound by ERalpha have distinct properties in terms of genome landscape, sequence features, and conservation compared with......-bound regions having a predominance of classical estrogen response elements (EREs) and GC-rich motifs. Differences in the properties of ER bound regions might explain some of the differences in gene expression programs and physiological effects shown by the respective estrogen receptors. Udgivelsesdato: 2008...

  8. Nearest-neighbor non-additivity versus long-range non-additivity in TATA-box structure and its implications for TBP-binding mechanism

    OpenAIRE

    Faiger, Hana; Ivanchenko, Marina; Haran, Tali E.

    2007-01-01

    TBP recognizes its target sites, TATA boxes, by recognizing their sequence-dependent structure and flexibility. Studying this mode of TATA-box recognition, termed ‘indirect readout’, is important for elucidating the binding mechanism in this system, as well as for developing methods to locate new binding sites in genomic DNA. We determined the binding stability and TBP-induced TATA-box bending for consensus-like TATA boxes. In addition, we calculated the individual information score of all st...

  9. Three-dimensional formulation of rigid-flexible multibody systems with flexible beam elements

    International Nuclear Information System (INIS)

    Multibody systems generally contain solids with appreciable deformations and which decisively influence the dynamics of the system. These solids have to be modeled by means of special formulations for flexible solids. At the same time, other solids are of such a high stiffness that they may be considered rigid, which simplifies their modeling. For these reasons, for a rigid-flexible multibody system, two types of formulations coexist in the equations of the system. Among the different possibilities provided in the literature on the material, the formulation in natural coordinates and the formulation in absolute nodal coordinates are utilized in this paper to model the rigid and flexible solids, respectively. This paper contains a mixed formulation based on the possibility of sharing coordinates between a rigid solid and a flexible solid. The global mass matrix of the system is shown to be constant and, in addition, many of the constraint equations obtained upon utilizing these formulations are linear and can be eliminated

  10. Flexibility-rigidity index for protein-nucleic acid flexibility and fluctuation analysis.

    Science.gov (United States)

    Opron, Kristopher; Xia, Kelin; Burton, Zach; Wei, Guo-Wei

    2016-05-30

    Protein-nucleic acid complexes are important for many cellular processes including the most essential functions such as transcription and translation. For many protein-nucleic acid complexes, flexibility of both macromolecules has been shown to be critical for specificity and/or function. The flexibility-rigidity index (FRI) has been proposed as an accurate and efficient approach for protein flexibility analysis. In this article, we introduce FRI for the flexibility analysis of protein-nucleic acid complexes. We demonstrate that a multiscale strategy, which incorporates multiple kernels to capture various length scales in biomolecular collective motions, is able to significantly improve the state of art in the flexibility analysis of protein-nucleic acid complexes. We take the advantage of the high accuracy and O(N) computational complexity of our multiscale FRI method to investigate the flexibility of ribosomal subunits, which are difficult to analyze by alternative approaches. An anisotropic FRI approach, which involves localized Hessian matrices, is utilized to study the translocation dynamics in an RNA polymerase. © 2016 Wiley Periodicals, Inc. PMID:26927815

  11. ENTHALPY-ENTROPY COMPENSATION AND COOPERATIVITY AS THERMODYNAMIC EPIPHENOMENA OF STRUCTURAL FLEXIBILITY IN LIGAND-RECEPTOR INTERACTIONS

    OpenAIRE

    Ferrante, Andrea; Gorski, Jack

    2012-01-01

    Ligand binding is a thermodynamically cooperative process in many biochemical systems characterized by the conformational flexibility of the reactants. However the contribution of conformational entropy to cooperativity of ligation needs to be elucidated. Here we perform kinetic and thermodynamic analyses on a panel of cycle-mutated peptides, derived from influenza H3 HA306-319, interacting with wild type and a mutant HLA-DR. We observe that within a certain range of peptide affinity, this sy...

  12. Water molecule network and active site flexibility of apo protein tyrosine phosphatase 1B

    DEFF Research Database (Denmark)

    Pedersen, A.K.; Peters, Günther H.J.; Møller, K.B.;

    2004-01-01

    Protein tyrosine phosphatase 1B (PTP1B) plays a key role as a negative regulator of insulin and leptin signalling and is therefore considered to be an important molecular target for the treatment of type 2 diabetes and obesity. Detailed structural information about the structure of PTP1B, including...... the conformation and flexibility of active-site residues as well as the water-molecule network, is a key issue in understanding ligand binding and enzyme kinetics and in structure-based drug design. A 1.95 Angstrom apo PTP1B structure has been obtained, showing four highly coordinated water molecules...

  13. Molecularly imprinted polymers: compromise between flexibility and rigidity for improving capture of template analogues.

    Science.gov (United States)

    Pardo, Antonelle; Mespouille, Laetitia; Dubois, Philippe; Blankert, Bertrand; Duez, Pierre

    2014-03-17

    New synthetic strategies for molecularly imprinted polymers (MIPs) were developed to mimic the flexibility and mobility exhibited by receptor/enzyme binding pockets. The MIPs were prepared by bulk polymerization with quercetin as template molecule, acrylamide as functional monomer, ethylene glycol dimethacrylate as cross-linker, and THF as porogen. The innovative grafting of specific oligoethylene glycol units onto the imprinted cavities allowed MIPs to be obtained that exhibit extended selectivity towards template analogues. This synthetic strategy gives promising perspectives for the design of molecular recognition of molecules based on a congruent pharmacophore, which should be of interest for drug development. PMID:24615923

  14. Polyaniline modified flexible conducting paper for cancer detection

    Science.gov (United States)

    Kumar, Saurabh; Sen, Anindita; Kumar, Suveen; Augustine, Shine; Yadav, Birendra K.; Mishra, Sandeep; Malhotra, Bansi D.

    2016-05-01

    We report results of studies relating to the fabrication of a flexible, disposable, and label free biosensing platform for detection of the cancer biomarker (carcinoembryonic antigen, CEA). Polyaniline (PANI) has been electrochemically deposited over gold sputtered paper (Au@paper) for covalent immobilization of monoclonal carcinoembryonic antibodies (anti-CEA). The bovine serum albumin (BSA) has been used for blocking nonspecific binding sites at the anti-CEA conjugated PANI/Au@Paper. The PANI/Au@Paper, anti-CEA/PANI/Au@Paper, and BSA/anti-CEA/PANI/Au@Paper platforms have been characterized using scanning electron microscopy, X-ray diffraction, Fourier transmission infrared spectroscopy, chronoamperometry, and electrochemical impedance techniques. The results of the electrochemical response studies indicate that this BSA/anti-CEA/PANI/Au@paper electrode has sensitivity of 13.9 μA ng-1 ml cm2, shelf life of 22 days, and can be used to estimate CEA in the range of 2-20 ng ml-1. This paper sensor has been validated by detection of CEA in serum samples of cancer patients via immunoassay technique.

  15. In Silico Investigation of the Neurotensin Receptor 1 Binding Site

    DEFF Research Database (Denmark)

    Lückmann, Michael; Holst, Birgitte; Schwartz, Thue W.;

    2016-01-01

    The neurotensin receptor 1 (NTSR1) belongs to the family of 7TM, G protein-coupled receptors, and is activated by the 13-amino-acid peptide neurotensin (NTS) that has been shown to play important roles in neurol. disorders and the promotion of cancer cells. Recently, a high-resoln. x-ray crystal...... structure of NTSR1 in complex with NTS8-13 has been detd., providing novel insights into peptide ligand recognition by 7TM receptors. SR48692, a potent and selective small mol. antagonist has previously been used extensively as a tool compd. to study NTSR1 receptor signaling properties. To investigate...... the binding mode of SR48692 and other small mol. compds. to NTSR1, we applied an Automated Ligand-guided Backbone Ensemble Receptor Optimization protocol (ALiBERO), taking receptor flexibility and ligand knowledge into account. Structurally overlapping binding poses for SR48692 and NTS8-13 were obsd., despite...

  16. Functional connectivity supporting the selective maintenance of feature-location binding in visual working memory.

    Science.gov (United States)

    Takahama, Sachiko; Saiki, Jun

    2014-01-01

    Information on an object's features bound to its location is very important for maintaining object representations in visual working memory. Interactions with dynamic multi-dimensional objects in an external environment require complex cognitive control, including the selective maintenance of feature-location binding. Here, we used event-related functional magnetic resonance imaging to investigate brain activity and functional connectivity related to the maintenance of complex feature-location binding. Participants were required to detect task-relevant changes in feature-location binding between objects defined by color, orientation, and location. We compared a complex binding task requiring complex feature-location binding (color-orientation-location) with a simple binding task in which simple feature-location binding, such as color-location, was task-relevant and the other feature was task-irrelevant. Univariate analyses showed that the dorsolateral prefrontal cortex (DLPFC), hippocampus, and frontoparietal network were activated during the maintenance of complex feature-location binding. Functional connectivity analyses indicated cooperation between the inferior precentral sulcus (infPreCS), DLPFC, and hippocampus during the maintenance of complex feature-location binding. In contrast, the connectivity for the spatial updating of simple feature-location binding determined by reanalyzing the data from Takahama et al. (2010) demonstrated that the superior parietal lobule (SPL) cooperated with the DLPFC and hippocampus. These results suggest that the connectivity for complex feature-location binding does not simply reflect general memory load and that the DLPFC and hippocampus flexibly modulate the dorsal frontoparietal network, depending on the task requirements, with the infPreCS involved in the maintenance of complex feature-location binding and the SPL involved in the spatial updating of simple feature-location binding. PMID:24917833

  17. Functional connectivity supporting the selective maintenance of feature-location binding in visual working memory

    Directory of Open Access Journals (Sweden)

    Sachiko eTakahama

    2014-06-01

    Full Text Available Information on an object’s features bound to its location is very important for maintaining object representations in visual working memory. Interactions with dynamic multi-dimensional objects in an external environment require complex cognitive control, including the selective maintenance of feature-location binding. Here, we used event-related functional magnetic resonance imaging to investigate brain activity and functional connectivity related to the maintenance of complex feature-location binding. Participants were required to detect task-relevant changes in feature-location binding between objects defined by color, orientation, and location. We compared a complex binding task requiring complex feature-location binding (color-orientation-location with a simple binding task in which simple feature-location binding, such as color-location, was task-relevant and the other feature was task-irrelevant. Univariate analyses showed that the dorsolateral prefrontal cortex (DLPFC, hippocampus, and frontoparietal network were activated during the maintenance of complex feature-location binding. Functional connectivity analyses indicated cooperation between the inferior precentral sulcus (infPreCS, DLPFC, and hippocampus during the maintenance of complex feature-location binding. In contrast, the connectivity for the spatial updating of simple feature-location binding determined by reanalyzing the data from Takahama et al. (2010 demonstrated that the superior parietal lobule (SPL cooperated with the DLPFC and hippocampus. These results suggest that the connectivity for complex feature-location binding does not simply reflect general memory load and that the DLPFC and hippocampus flexibly modulate the dorsal frontoparietal network, depending on the task requirements, with the infPreCS involved in the maintenance of complex feature-location binding and the SPL involved in the spatial updating of simple feature-location binding.

  18. Flexible Skins Containing Integrated Sensors and Circuitry

    Science.gov (United States)

    Liu, Chang

    2007-01-01

    Artificial sensor skins modeled partly in imitation of biological sensor skins are undergoing development. These sensor skins comprise flexible polymer substrates that contain and/or support dense one- and two-dimensional arrays of microscopic sensors and associated microelectronic circuits. They afford multiple tactile sensing modalities for measuring physical phenomena that can include contact forces; hardnesses, temperatures, and thermal conductivities of objects with which they are in contact; and pressures, shear stresses, and flow velocities in fluids. The sensor skins are mechanically robust, and, because of their flexibility, they can be readily attached to curved and possibly moving and flexing surfaces of robots, wind-tunnel models, and other objects that one might seek to equip for tactile sensing. Because of the diversity of actual and potential sensor-skin design criteria and designs and the complexity of the fabrication processes needed to realize the designs, it is not possible to describe the sensor-skin concept in detail within this article.

  19. The motion of interconnected flexible bodies

    Science.gov (United States)

    Hopkins, A. S.

    1975-01-01

    The equations of motion for an arbitrarily interconnected collection of substructures are derived. The substructures are elastic bodies which may be idealized as finite element assemblies and are subject to small deformations relative to a nominal state. Interconnections between the elastic substructures permit large relative translations and rotations between substructures, governed by Pfaffian constraints describing the connections. Screw connections (permitting rotation about and translation along a single axis) eliminate constraint forces and incorporate modal coupling. The problem of flexible spacecraft simulation is discussed. Hurty's component mode approach is extended by permitting interconnected elastic substructures large motions relative to each other and relative to inertial space. The hybrid coordinate methods are generalized by permitting all substructures to be flexible (rather than only the terminal members of a topological tree of substructures). The basic relationships of continuum mechanics are developed.

  20. Tuned mass absorber on a flexible structure

    DEFF Research Database (Denmark)

    Krenk, Steen; Høgsberg, Jan Becker

    2014-01-01

    The classic design of a tuned mass absorber is based on a simple two-mass analogy in which the tuned mass is connected to the structural mass with a spring and a viscous damper. In a flexible multi-degree-of-freedom structure the tuned mass absorber is typically introduced to provide damping of a...... specific mode. The motion of the point of attachment of the tuned mass absorber to the structure has not only a contribution from the targeted mode, but also a background contribution from other non-resonant modes. Similarly, the force provided by the tuned mass absorber is distributed between the targeted...... mode and the background modes. It is demonstrated how this effect can be included via a non-dimensional dynamic background flexibility coefficient, extracted from a classic modal analysis for the particular frequency of the selected mode. An explicit calibration procedure is developed starting with the...

  1. Screen-printed flexible MRI receive coils

    Science.gov (United States)

    Corea, Joseph R.; Flynn, Anita M.; Lechêne, Balthazar; Scott, Greig; Reed, Galen D.; Shin, Peter J.; Lustig, Michael; Arias, Ana C.

    2016-03-01

    Magnetic resonance imaging is an inherently signal-to-noise-starved technique that limits the spatial resolution, diagnostic image quality and results in typically long acquisition times that are prone to motion artefacts. This limitation is exacerbated when receive coils have poor fit due to lack of flexibility or need for padding for patient comfort. Here, we report a new approach that uses printing for fabricating receive coils. Our approach enables highly flexible, extremely lightweight conforming devices. We show that these devices exhibit similar to higher signal-to-noise ratio than conventional ones, in clinical scenarios when coils could be displaced more than 18 mm away from the body. In addition, we provide detailed material properties and components performance analysis. Prototype arrays are incorporated within infant blankets for in vivo studies. This work presents the first fully functional, printed coils for 1.5- and 3-T clinical scanners.

  2. Elastomeric composites for flexible microwave substrates

    Science.gov (United States)

    Awang, Robiatun A.; Baum, Thomas; Berean, Kyle J.; Yi, Pyshar; Kalantar-zadeh, Kourosh; Sriram, Sharath; Rowe, Wayne S. T.

    2016-03-01

    Manipulating dielectric properties of polydimethylsiloxane (PDMS) is an important consideration for flexible, low-loss device design. This paper presents a method for reducing dielectric loss (tan δ) by forming PDMS composites loaded with various concentrations of either alumina (Al2O3) or polytetrafluoroethylene (PTFE) particles. The structural, mechanical, and electrical properties of the composites are investigated. Theoretical mixing models were used to predict the relative permittivity (ɛr) of PDMS composites, and good similarity with the measured ɛr was observed. The incorporation of either low dielectric loss filler in the PDMS matrix (up to 50 wt. % filler loading) is shown to reduce the dielectric loss while maintaining the flexibility of the host matrix. The fillers can also control the permittivity of the composite, either increasing or decreasing relative permittivity from that of PDMS. Interestingly, a strain of ˜500% can be applied to 15 wt. % PDMS/PTFE composites, compared with ˜350% for pure PDMS.

  3. Screen-printed flexible MRI receive coils.

    Science.gov (United States)

    Corea, Joseph R; Flynn, Anita M; Lechêne, Balthazar; Scott, Greig; Reed, Galen D; Shin, Peter J; Lustig, Michael; Arias, Ana C

    2016-01-01

    Magnetic resonance imaging is an inherently signal-to-noise-starved technique that limits the spatial resolution, diagnostic image quality and results in typically long acquisition times that are prone to motion artefacts. This limitation is exacerbated when receive coils have poor fit due to lack of flexibility or need for padding for patient comfort. Here, we report a new approach that uses printing for fabricating receive coils. Our approach enables highly flexible, extremely lightweight conforming devices. We show that these devices exhibit similar to higher signal-to-noise ratio than conventional ones, in clinical scenarios when coils could be displaced more than 18 mm away from the body. In addition, we provide detailed material properties and components performance analysis. Prototype arrays are incorporated within infant blankets for in vivo studies. This work presents the first fully functional, printed coils for 1.5- and 3-T clinical scanners. PMID:26961073

  4. MATERIAL HANDLING IN FLEXIBLE MANUFACTURING SYSTEM

    Directory of Open Access Journals (Sweden)

    Mr. Neeraj Dahiya

    2011-08-01

    Full Text Available The objective of this study is to analyze the system performanceof a flexible manufacturing system cell. The study givesinformation on production potential of the cell by groupingcommon parts. To complete this, computer simulation models aredeveloped using the SIMAN simulation language. Initially nomaterial handling is provided to the manufacturing system to getan upper bound estimate of production output. Next, we explorethe impact that an automatic guided vehicle (AGV has on systemperformance with manufacturing system. The final analysis isperformed in which a conveyor is implemented for the materialhandling. The performance result with comparison is presented inthe form of confidence intervals. After examine the simulationresults, we recommend to implement a conveyor system formaterial handling. Use of AGV in the flexible manufacturingsystem creates a bottleneck which causes a dramatically decreasein the production: as compare to a conveyor as the materialhandling system which does not limit the daily production outputof the manufacturing cell.

  5. Supporting Flexible Data Distribution in Software DSMs

    Institute of Scientific and Technical Information of China (English)

    洪锦伟; 陈国良; 张兆庆

    2000-01-01

    Page-based software DSM systems suffer from false sharing caused by the large sharing granularity, and only support one-dimension Block or Cyclic block data distribution schemes. Thus applications running on them will suffer from poor data locality and will be able to exploit parallelism only when using a large number of processors. In this paper, a way towards supporting flexible data distribu tion (FDD) on software DSM system is presented. Small granularity-tunable blocks, the size of which can be set by compiler or programmer, are used to overlap the working data sets distributed among processors. The FDD was implemented on a software DSM system called JIAJiA. Compared with Block/Cyclic-block distribution schemes used by most DSM systems now, experiments show that the proposed way of flexible data distribution is more effective. The performance of the applications used in the experiments is significantly improved.

  6. Flexible Bit Preservation on a National Basis

    DEFF Research Database (Denmark)

    Jurik, Bolette; Nielsen, Anders Bo; Zierau, Eld

    2012-01-01

    In this paper we present the results from The Danish National Bit Repository project. The project aim was establishment of a system that can offer flexible and sustainable bit preservation solutions to Danish cultural heritage institutions. Here the bit preservation solutions must include support...... of bit safety as well as other requirements like e.g. confidentiality and availability. The Danish National Bit Repository is motivated by the need to investigate and handle bit preservation for digital cultural heritage. Digital preservation relies on the integrity of the bits which digital material...... consists of, and it is with this focus that the project was initiated. This paper summarizes the requirements for a general system to offer bit preservation to cultural heritage institutions. On this basis the paper describes the resulting flexible system which can support such requirements. The paper will...

  7. Tuning strain in flexible graphene nanoelectromechanical resonators

    Science.gov (United States)

    Guan, Fen; Kumaravadivel, Piranavan; Averin, Dmitri V.; Du, Xu

    2015-11-01

    The structural flexibility of low dimensional nanomaterials offers unique opportunities for studying the impact of strain on their physical properties and for developing innovative devices utilizing strain engineering. A key towards such goals is a device platform which allows the independent tuning and reliable calibration of the strain. Here, we report the fabrication and characterization of graphene nanoelectromechanical resonators (GNEMRs) on flexible substrates. Combining substrate bending and electrostatic gating, we achieve the independent tuning of the strain and sagging in graphene and explore the nonlinear dynamics over a wide parameter space. Analytical and numerical studies of a continuum mechanics model, including the competing higher order nonlinear terms, reveal a comprehensive nonlinear dynamics phase diagram, which quantitatively explains the complex behaviors of GNEMRs.

  8. Wave-induced dynamics of flexible blades

    CERN Document Server

    Luhar, M

    2015-01-01

    We present an experimental and numerical study that describes the motion of flexible blades, scaled to be dynamically similar to natural aquatic vegetation, forced by wave-induced oscillatory flows. For the conditions tested, blade motion is governed primarily by two dimensionless variables: (i) the Cauchy number, $Ca$, which represents the ratio of the hydrodynamic forcing to the restoring force due to blade stiffness, and (ii) the ratio of the blade length to the wave orbital excursion, $L$. For flexible blades with $Ca \\gg 1$, the relationship between drag and velocity can be described by two different scaling laws at the large- and small-excursion limits. For large excursions ($L \\ll 1$), the flow resembles a unidirectional current and the scaling laws developed for steady-flow reconfiguration studies hold. For small excursions ($L \\gg 1$), the beam equations may be linearized and a different scaling law for drag applies. The experimental force measurements suggest that the small-excursion scaling applies...

  9. A flexible sequential Gaussian simulation program: USGSIM

    Science.gov (United States)

    Manchuk, John G.; Deutsch, Clayton V.

    2012-04-01

    Sequential Gaussian simulation is a widely used algorithm for the stochastic characterization of properties from various earth science disciplines. Many variants have been developed to deal with the increasing complexity of modeling applications. The program described in this paper is a flexible, tested, and documented implementation. Multiple variables can be cosimulated within different rock types simultaneously. The stepwise transform is integrated into the program as are collocated cokriging, collocated cokriging with the intrinsic model, and cokriging with a linear model of coregionalization for the cosimulation of multiple variables. Multiple secondary data can be incorporated using locally varying means, collocated cokriging, and Bayesian updating. The search options and other parameters are flexible within rock types. Fortran source code and a compiled executable are provided.

  10. A Formation Method of Flexible Learning Objects

    Directory of Open Access Journals (Sweden)

    Ramunas KUBILIUNAS

    2009-04-01

    Full Text Available A brief overview of formation method of flexible learning objects is presented in this article. The basis of this method is e-learning material that is structured and separated from display rules. The learning objects that have such e-learning material may be adapted to individual needs and may be used in different learning contexts without changing e-learning material. To change the presentation form of e-learning material of such objects, it is enough to change display rules of this material. However, if the e-learning material must be adapted too, it is much easier to do this as the material is structured and contains less technical information of representation. The adaptation of such learning objects is more effective and needs less work time input, therefore they are called as flexible learning objects.

  11. Procedural Optimization Models for Multiobjective Flexible JSSP

    Directory of Open Access Journals (Sweden)

    Elena Simona NICOARA

    2013-01-01

    Full Text Available The most challenging issues related to manufacturing efficiency occur if the jobs to be sched-uled are structurally different, if these jobs allow flexible routings on the equipments and mul-tiple objectives are required. This framework, called Multi-objective Flexible Job Shop Scheduling Problems (MOFJSSP, applicable to many real processes, has been less reported in the literature than the JSSP framework, which has been extensively formalized, modeled and analyzed from many perspectives. The MOFJSSP lie, as many other NP-hard problems, in a tedious place where the vast optimization theory meets the real world context. The paper brings to discussion the most optimization models suited to MOFJSSP and analyzes in detail the genetic algorithms and agent-based models as the most appropriate procedural models.

  12. How Beyond Rule of 5 Drugs and Clinical Candidates Bind to Their Targets.

    Science.gov (United States)

    Doak, Bradley C; Zheng, Jie; Dobritzsch, Doreen; Kihlberg, Jan

    2016-03-24

    To improve discovery of drugs for difficult targets, the opportunities of chemical space beyond the rule of 5 (bRo5) were examined by retrospective analysis of a comprehensive set of structures for complexes between drugs and clinical candidates and their targets. The analysis illustrates the potential of compounds far beyond rule of 5 space to modulate novel and difficult target classes that have large, flat, and groove-shaped binding sites. However, ligand efficiencies are significantly reduced for flat- and groove-shape binding sites, suggesting that adjustments of how to use such metrics are required. Ligands bRo5 appear to benefit from an appropriate balance between rigidity and flexibility to bind with sufficient affinity to their targets, with macrocycles and nonmacrocycles being found to have similar flexibility. However, macrocycles were more disk- and spherelike, which may contribute to their superior binding to flat sites, while rigidification of nonmacrocycles lead to rodlike ligands that bind well to groove-shaped binding sites. These insights should contribute to altering perceptions of what targets are considered "druggable" and provide support for drug design in beyond rule of 5 space. PMID:26457449

  13. Behavioral flexibility and problem solving in an invasive bird

    Science.gov (United States)

    2016-01-01

    Behavioral flexibility is considered an important trait for adapting to environmental change, but it is unclear what it is, how it works, and whether it is a problem solving ability. I investigated behavioral flexibility and problem solving experimentally in great-tailed grackles, an invasive bird species and thus a likely candidate for possessing behavioral flexibility. Grackles demonstrated behavioral flexibility in two contexts, the Aesop’s Fable paradigm and a color association test. Contrary to predictions, behavioral flexibility did not correlate across contexts. Four out of 6 grackles exhibited efficient problem solving abilities, but problem solving efficiency did not appear to be directly linked with behavioral flexibility. Problem solving speed also did not significantly correlate with reversal learning scores, indicating that faster learners were not the most flexible. These results reveal how little we know about behavioral flexibility, and provide an immense opportunity for future research to explore how individuals and species can use behavior to react to changing environments.

  14. Irreversible Investment, Operating Flexibility, and Time Lags

    OpenAIRE

    Takashima, Ryuta; GOTO, MAKOTO; Tsujimura, Motoh

    2010-01-01

    We consider an optimal investment problem when a firm such as an electric power company has the operational flexibility to expand and contract capacity with fixed cost. This problem is formulated as an impulse control problem combined with optimal stopping. Consequently, we obtain optimal investment timing, optimal capacity expansion and contraction timing, and the investment value. We also show investment, capacity expansion and contraction rule are influenced by the price volatility and the...

  15. IRREVERSIBLE INVESTMENT, OPERATING FLEXIBILITY, AND TIME LAGS

    OpenAIRE

    RYUTA TAKASHIMA; MAKOTO GOTO; MOTOH TSUJIMURA

    2010-01-01

    We consider an optimal investment problem when a firm such as an electric power company has the operational flexibility to expand and contract capacity with fixed cost. This problem is formulated as an impulse control problem combined with optimal stopping. Consequently, we obtain optimal investment timing, optimal capacity expansion and contraction timing, and the investment value. We also show investment, capacity expansion and contraction rule are influenced by the price volatility and the...

  16. Flexible planning strategies of sustainable city development

    OpenAIRE

    Živković Milica; Jovanović Goran; Kondić Slaviša

    2014-01-01

    Recognizing the uncertainty of future city development is the basic starting point of sustainable urban planning. Flexibility in design enables the development of “age-friendly cities”, reducing the risks of functional failures caused by changing living circumstances. Planning strategies that enable adaptation of the system to changing environmental, functional, social and technological conditions are essential to the long service life and the resilience of...

  17. Flexible resources allocation techniques: characteristics and modelling

    OpenAIRE

    Attia, El-Awady; Edi, Kouassi Hilaire; Duquenne, Philippe

    2012-01-01

    International audience At the interface between engineering, economics, social sciences and humanities, industrial engineering aims to provide answers to various sectors of business problems. One of these problems is the adjustment between the workload needed by the work to be realised and the availability of the company resources. The objective of this work is to help to find a methodology for the allocation of flexible human resources in industrial activities planning and scheduling. Thi...

  18. Flexible working time arrangements in Romania

    OpenAIRE

    Elena ZAMFIR

    2010-01-01

    The objective of the paper is the analysis of women-related issues focusing on labour market policies and work flexibility in Romania. The active internal dynamics regarding gender issues are strongly influenced by the national and international political processes, especially those incorporating the necessary changes of European and international labour regulations. Anti-discriminatory strategies need to be promoted at every level of society. Sources of information are: literature in the fie...

  19. A flexible BIST strategy for SDR transmitters

    OpenAIRE

    Dogaru, Emanuel; Vinci Dos Santos, Filipe; Rebernak, William

    2014-01-01

    Software-defined radio (SDR) development aims for increased speed and flexibility. The advent of these system level requirements on the physical layer (PHY) access hardware is leading to more complex architectures, which together with higher levels of integration pose a challenging problem for product testing. For radio units that must be field-upgradeable without specialized equipment, Built-in Self-Test (BIST) schemes are arguably the only way to ensure continued compliance to specification...

  20. Imposing Theoretical Regularity on Flexible Functional Forms

    OpenAIRE

    Apostolos Serletis; Guohua Feng

    2013-01-01

    In this paper we build on work by Gallant and Golub (1984), Diewert and Wales (1987), and Barnett (2002) and provide a comparison among three different methods of imposing theoretical regularity on flexible functional forms - reparameterization using Cholesky factorization, constrained optimization, and Bayesian methodology. We apply the methodology to a translog cost and share equation system and make a distinction between local, regional, pointwise, and global regularity. We find that the i...

  1. Flexible Learning Itineraries Based on Conceptual Maps

    OpenAIRE

    Olga Lucía Agudelo; Jesus Salinas Ibáñez

    2015-01-01

    The use of learning itineraries based on conceptual maps is studied in order to propose a more flexible instructional design that strengthens the learning process focused on the student, generating non-linear processes, characterising its elements, setting up relationships between them and shaping a general model with specifications for each educational level. Through a methodology built on the Successive Approximation Model (SAM), this article studies learning itinerary design, implementatio...

  2. Flexible feature interface for multimedia sources

    Science.gov (United States)

    Coffland, Douglas R.

    2009-06-09

    A flexible feature interface for multimedia sources system that includes a single interface for the addition of features and functions to multimedia sources and for accessing those features and functions from remote hosts. The interface utilizes the export statement: export "C" D11Export void FunctionName(int argc, char ** argv,char * result, SecureSession *ctrl) or the binary equivalent of the export statement.

  3. Wearable magnetic field sensors for flexible electronics.

    Science.gov (United States)

    Melzer, Michael; Mönch, Jens Ingolf; Makarov, Denys; Zabila, Yevhen; Cañón Bermúdez, Gilbert Santiago; Karnaushenko, Daniil; Baunack, Stefan; Bahr, Falk; Yan, Chenglin; Kaltenbrunner, Martin; Schmidt, Oliver G

    2015-02-18

    Highly flexible bismuth Hall sensors on polymeric foils are fabricated, and the key optimization steps that are required to boost their sensitivity to the bulk value are identified. The sensor can be bent around the wrist or positioned on the finger to realize an interactive pointing device for wearable electronics. Furthermore, this technology is of great interest for the rapidly developing market of -eMobility, for optimization of eMotors and magnetic bearings. PMID:25523752

  4. Multifunctional Weft-knitted Flexible Composite Structures

    OpenAIRE

    Ali, S.; Fernando, A.; Potluri, P.;Gresil, M.

    2015-01-01

    Multifunctional composite structures offer unique features such as the ability to sense and respond to external stimuli that can be force, pressure or temperature. Knitted preforms are popular due their excellent properties like flexibility and impact resistance. So far, most of the research is focused on mechanical properties of rigid knitted composites structures. In this research, a preform for a composite is produced having a knitted functional sensing layer constructed using stainless st...

  5. A More Flexible Lipoprotein Sorting Pathway

    OpenAIRE

    Chahales, Peter; Thanassi, David G.

    2015-01-01

    Lipoprotein biogenesis in Gram-negative bacteria occurs by a conserved pathway, each step of which is considered essential. In contrast to this model, LoVullo and colleagues demonstrate that the N-acyl transferase Lnt is not required in Francisella tularensis or Neisseria gonorrhoeae. This suggests the existence of a more flexible lipoprotein pathway, likely due to a modified Lol transporter complex, and raises the possibility that pathogens may regulate lipoprotein processing to modulate int...

  6. Six principles to enhance health workforce flexibility

    OpenAIRE

    Nancarrow, Susan A

    2015-01-01

    Abstract This paper proposes approaches to break down the boundaries that reduce the ability of the health workforce to respond to population needs, or workforce flexibility. Accessible health services require sufficient numbers and types of skilled workers to meet population needs. However, there are several reasons that the health workforce cannot or does not meet population needs. These primarily stem from workforce shortages. However, the health workforce can also be prevented from respon...

  7. Capacity of Deep Water Flexible Risers

    OpenAIRE

    Thorsen, Mats Jørgen

    2011-01-01

    A flexible riser operating in deep water will experience large environmental forces. Due to the length of the riser, dynamic tension from drag forces will be substantial. The tensile force is transferred directly into the end fitting, and fatigue damage may be critical at this point. In addition, the large hydrostatic pressure at the ocean floor may induce compressive stresses in the tensile armor, something this component is not designed for. In connection with these challenges, this thesis ...

  8. Calculation Methodology for Flexible Arithmetic Processing

    OpenAIRE

    García Chamizo, Juan Manuel; Mora Pascual, Jerónimo Manuel; Mora Mora, Higinio; Signes Pont, María Teresa

    2003-01-01

    A new operation model of flexible calculation that allows us to adjust the operation delay depending on the available time is presented. The operation method design uses look-up tables and progressive construction of the result. The increase in the operators’ granularity opens up new possibilities in calculation methods and microprocessor design. This methodology, together with the advances in technology, enables the functions of an arithmetic unit to be implemented on the basis of techniques...

  9. Time-Precision Flexible Arithmetic Unit

    OpenAIRE

    García Chamizo, Juan Manuel; Mora Pascual, Jerónimo Manuel; Mora Mora, Higinio; Signes Pont, María Teresa

    2003-01-01

    A new conception of flexible calculation that allows us to adjust an operation depending on the available time computation is presented. The proposed arithmetic unit is based on this principle. It contains a control operation module that determines the process time of each calculation. The operation method design uses precalculated data stored in look-up tables, which provide, above all, quality results and systematization in the implementation of low level primitives that set parameters for ...

  10. Flexible Receivers in CMOS for Wireless Communication

    OpenAIRE

    Nejdel, Anders

    2015-01-01

    Consumers are pushing for higher data rates to support more services that are introduced in mobile applications. As an example, a few years ago video-on-demand was only accessed through landlines, but today wireless devices are frequently used to stream video. To support this, more flexible network solutions have merged in 4G, introducing new technical problems to the mobile terminal. New techniques are thus needed, and this dissertation explores five different ideas for receiver front-ends, ...

  11. CONCEPT OF ADVANCED FLEXIBLE USE OF AIRSPACE

    Directory of Open Access Journals (Sweden)

    Oleksandr Luppo

    2016-06-01

    Full Text Available Purpose: Concept of Flexible Use of Airspace (FUA allows to eliminate many problems on the basis of civil-military coordination, but there are still a lot of areas for improvement. These improvements will be implemented in the Advanced Flexible Use of Airspace (AFUA concept. Methods: We examine the airspace structure in the frames of AFUA concept, which includes variable profile areas, temporary reserved and temporary segregated areas, danger or restricted areas. Mission Trajectory in AFUA which allows designing ad-hoc structures delineation at short notice is also examined. Regarding the performance enhancements of AFUA we compare these with FUA concept. Examination of AFUA structure gives us better view of the functions and opportunities of this concept. Result: AFUA concept provides many advantages for the civil aviation stakeholders and includes many other positive sides. Variable Profile Areas provide more flexibility, particularly in a high density traffic area and any combination of basic volume possible. Collaborative decision-making will increase the situational awareness of both parties and help to decrease the transit between airbases and training areas, allows military to use larger airspaces for missions on an absolute time-limited basis. As a result of mission trajectory implementation in AFUA concept general air traffic crossing are possible in all type of airspace structures, after coordination or under specific permanent agreements. The use of Centralized AFUA Services will allow the central collection, integration and provision of ASM data in support of continuous collaborative network processes, in such a way improving operational performance during the planning and execution phases (predictability, flexibility, better use of capacity, enhanced flight efficiency, real time sharing of information, better management of available airspace. Discussion: Given the important contribution that AFUA brings into air traffic management

  12. Simulating charge transport in flexible systems

    Directory of Open Access Journals (Sweden)

    Timothy Clark

    2015-12-01

    Full Text Available Systems in which movements occur on two significantly different time domains, such as organic electronic components with flexible molecules, require different simulation techniques for the two time scales. In the case of molecular electronics, charge transport is complicated by the several different mechanisms (and theoretical models that apply in different cases. We cannot yet combine time scales of molecular and electronic movement in simulations of real systems. This review describes our progress towards this goal.

  13. Simulating charge transport in flexible systems

    OpenAIRE

    Timothy Clark

    2015-01-01

    Systems in which movements occur on two significantly different time domains, such as organic electronic components with flexible molecules, require different simulation techniques for the two time scales. In the case of molecular electronics, charge transport is complicated by the several different mechanisms (and theoretical models) that apply in different cases. We cannot yet combine time scales of molecular and electronic movement in simulations of real systems. This review describes our ...

  14. FAME - A Flexible Appearance Modelling Environment

    OpenAIRE

    Stegmann, Mikkel Bille; Ersbøll, Bjarne Kjær; Larsen, Rasmus

    2003-01-01

    Combined modelling of pixel intensities and shape has proven to be a very robust and widely applicable approach to interpret images. As such the Active Appearance Model (AAM) framework has been applied to a wide variety of problems within medical image analysis. This paper summarises AAM applications within medicine and describes a public domain implementation, namely the Flexible Appearance Modelling Environment (FAME). We give guidelines for the use of this research platform, and show that ...

  15. Flexible Tactile Sensor Using Polyurethane Thin Film

    OpenAIRE

    Seiji Aoyagi; Tomokazu Takahashi; Masato Suzuki

    2012-01-01

    A novel capacitive tactile sensor using a polyurethane thin film is proposed in this paper. In previous studies, capacitive tactile sensors generally had an air gap between two electrodes in order to enhance the sensitivity. In this study, there is only polyurethane thin film and no air gap between the electrodes. The sensitivity of this sensor is higher than the previous capacitive tactile sensors because the polyurethane is a fairly flexible elastomer and the film is very thin (about 1 µm)....

  16. Reliable control of large scale flexible structures

    Czech Academy of Sciences Publication Activity Database

    Bakule, Lubomír

    Gdansk : IFAC - GUT, 2007, s. 1-6. ISSN 1367-5788. [IFAC/IFORS/IMACS/IFIP Symposium on Large Scale Complex Systems: Theory and Applications /11./. Gdansk (PL), 23.07.2007-25.07.2007] R&D Projects: GA AV ČR IAA2075304; GA MŠk(CZ) LA 282 Institutional research plan: CEZ:AV0Z10750506 Keywords : decentralized control * large scale systems * decomposition * reliability * flexible structures * redundancy Subject RIV: BC - Control Systems Theory

  17. A Flexible Framework for Magnetic Measurements

    OpenAIRE

    Inglese, Vitaliano

    2009-01-01

    The work presented in this Ph.D. thesis covers the specification, design, prototyping, and validation of a new version of a magnetic measurement control, acquisition, and data analysis software package: the Flexible Framework for Magnetic Measurements (FFMM). FFMM constitutes the software part of the new platform for magnetic measurements, including also new high-performance hardware, developed at the European Organization for Nuclear Research (CERN) in cooperation with the Department of Engi...

  18. Informal and Formal Labour Flexibility in Mexico

    OpenAIRE

    Alcaraz Carlo

    2009-01-01

    We address a vital topic about labour markets in developing countries: The flexibility of the formal and informal labour sectors. For the Mexican economy, we use a panel from the National Survey of Urban Employment (ENEU) from 1995 to 2001. We control for workers´ observable characteristics, sample selection and non observable regional heterogeneity. We also take into account the possible endogeneity of the unemployment rate. The results show evidence that in the formal sector, unemployment d...

  19. Towards Modeling Changeovers for flexible foundry manufacturing

    OpenAIRE

    Rhythm Suren Wadhwa

    2012-01-01

    Costs involved in implementing manufacturing flexibility to meet customer demand are more important in the SMEs, especially that are labor intensive for example metalcasting companies located in a high cost country like Norway. Changeover is an important issue in foundries, and setup time of the dies is one of the vital parameters in the manufacturing process. This paper presents an analytical method for evaluating product changeover suitable for foundry manufacturing environment.

  20. A more flexible lipoprotein sorting pathway.

    Science.gov (United States)

    Chahales, Peter; Thanassi, David G

    2015-05-01

    Lipoprotein biogenesis in Gram-negative bacteria occurs by a conserved pathway, each step of which is considered essential. In contrast to this model, LoVullo and colleagues demonstrate that the N-acyl transferase Lnt is not required in Francisella tularensis or Neisseria gonorrhoeae. This suggests the existence of a more flexible lipoprotein pathway, likely due to a modified Lol transporter complex, and raises the possibility that pathogens may regulate lipoprotein processing to modulate interactions with the host. PMID:25755190

  1. The illness flexibility model and sickness absence

    OpenAIRE

    Johansson, Gun

    2007-01-01

    Research on sickness absence has repeatedly been described as theoretically undeveloped. In this thesis the model of illness flexibility is introduced. In this model, sickness absence is assumed to be caused by people’s ability and motivation to work. Ability and motivation will in turn be affected by conditions met in and outside work. In the model, five basic components are discerned describing such conditions. Adjustment latitude describes opportunities to adjust work to ...

  2. MATERIAL HANDLING IN FLEXIBLE MANUFACTURING SYSTEM

    OpenAIRE

    Mr. Neeraj Dahiya; Mr. Neeraj Nirmal

    2011-01-01

    The objective of this study is to analyze the system performanceof a flexible manufacturing system cell. The study givesinformation on production potential of the cell by groupingcommon parts. To complete this, computer simulation models aredeveloped using the SIMAN simulation language. Initially nomaterial handling is provided to the manufacturing system to getan upper bound estimate of production output. Next, we explorethe impact that an automatic guided vehicle (AGV) has on systemperforma...

  3. Flexible contracts and human capital investments

    OpenAIRE

    Fouarge, D.; Grip, A. de; Smits, W.; de Vries, M R

    2011-01-01

    As suggested by human capital theory, workers with flexible contracts participate lessoften in training than those with permanent contracts. We find that this is merely dueto the fact that flexworkers receive less employer–funded training, a gap they can onlypartly compensate for by their own training investments. Flexworkers particularlyparticipate less in firm–specific training that is meant to keep up with new skilldemands than workers with permanent contracts. However, for those who parti...

  4. Aggregate expenditure ceilings and allocative flexibility

    OpenAIRE

    Marc Robinson

    2012-01-01

    This article shows how to combine top-down budgeting – in the core sense of the establishment of a hard aggregate expenditure ceiling at the start of the budget preparation process – with flexibility in the allocation of the aggregate ceiling between spending ministries during budget preparation. It argues strongly against determining spending ministry shares of the aggregate expenditure ceiling without any prior opportunity for them to present formal new spending proposals. The keys to recon...

  5. Flexible work and immigration in Europe

    OpenAIRE

    Raess, D.; Burgoon, B.

    2013-01-01

    Immigration has risen substantially in many European economies, with far-reaching if still uncertain implications for labour markets and industrial relations. This article investigates such implications, focusing on employment flexibility, involving both ‘external flexibility’ (fixed-term or temporary agency and/or involuntary part-time work) and ‘internal flexibility’ (overtime and/or balancing-time accounts). The article identifies reasons why immigration should generally increase the incid...

  6. Quarkonium Binding and Entropic Force

    CERN Document Server

    Satz, Helmut

    2015-01-01

    A Q-Qbar bound state represents a balance between repulsive kinetic and attractive potential energy. In a hot quark-gluon plasma, the interaction potential experiences medium effects. Color screening modifies the attractive binding force between the quarks, while the increase of entropy with Q-Qbar separation gives rise to a growing repulsion. We study the role of these phenomena for in-medium Q-Qbar binding and dissociation. It is found that the relevant potential for Q-Qbar binding is the free energy F; with increasing Q-Qbar separation, further binding through the internal energy U is compensated by repulsive entropic effects.

  7. A regulatory hydrophobic area in the flexible joint region of plasminogen activator inhibitor-1, defined with fluorescent activity-neutralizing ligands. Ligand-induced serpin polymerization

    DEFF Research Database (Denmark)

    Egelund, R; Einholm, A P; Pedersen, K E; Nielsen, R W; Christensen, A; Deinum, J; Andreasen, Peter A

    2001-01-01

    all tested nonfluorescent neutralizers, indicating that all neutralizers bind to a common hydrophobic area preferentially accessible in active PAI-1. Activity neutralization proceeded through two consecutive steps as follows: first step is conversion to forms displaying substrate behavior toward u...... having overlapping, but not identical, binding sites in the region around alpha-helices D and E and beta-strand 1A, known to act as a flexible joint when beta-sheet A opens and the reactive center loop inserts as beta-strand 4A during reaction with target proteinases. The defined binding area may be a...

  8. Molecular Basis for the Cu2+ Binding-Induced Destabilization of β2-Microglobulin Revealed by Molecular Dynamics Simulation

    OpenAIRE

    Deng, Nan-Jie; Yan, Lisa; Singh, Deepak; Cieplak, Piotr

    2006-01-01

    According to experimental data, binding of the Cu2+ ions destabilizes the native state of β2-microglobulin (β2m). The partial unfolding of the protein was generally considered an early step toward fibril formation in dialysis-related amyloidosis. Recent NMR studies have suggested that the destabilization of the protein might be achieved through increased flexibility upon Cu2+ binding. However, the molecular mechanism of destabilization due to Cu2+, its role in amyloid formation, and the relat...

  9. Silk Fibroin for Flexible Electronic Devices.

    Science.gov (United States)

    Zhu, Bowen; Wang, Hong; Leow, Wan Ru; Cai, Yurong; Loh, Xian Jun; Han, Ming-Yong; Chen, Xiaodong

    2016-06-01

    Flexible electronic devices are necessary for applications involving unconventional interfaces, such as soft and curved biological systems, in which traditional silicon-based electronics would confront a mechanical mismatch. Biological polymers offer new opportunities for flexible electronic devices by virtue of their biocompatibility, environmental benignity, and sustainability, as well as low cost. As an intriguing and abundant biomaterial, silk offers exquisite mechanical, optical, and electrical properties that are advantageous toward the development of next-generation biocompatible electronic devices. The utilization of silk fibroin is emphasized as both passive and active components in flexible electronic devices. The employment of biocompatible and biosustainable silk materials revolutionizes state-of-the-art electronic devices and systems that currently rely on conventional semiconductor technologies. Advances in silk-based electronic devices would open new avenues for employing biomaterials in the design and integration of high-performance biointegrated electronics for future applications in consumer electronics, computing technologies, and biomedical diagnosis, as well as human-machine interfaces. PMID:26684370

  10. Viscous pumping inspired by flexible propulsion

    CERN Document Server

    Arco, Roger M; Lauga, Eric; Zenit, Roberto

    2014-01-01

    Fluid-suspended microorganisms have evolved different swimming and feeding strategies in order to cope with an environment dominated by viscous effects. For instance ciliated organisms rely on the collective motion of flexible appendices to move and feed. By performing a non-reciprocal motion, flexible filaments can produce a net propulsive force, or pump fluid, in the absence of inertia. Inspired by such fundamental concept, we propose a strategy to produce macroscopic pumping and mixing in creeping flow. We measure experimentally the net motion of a Newtonian viscous fluid induced by the reciprocal motion of a flapper. When the flapper is rigid no net motion is induced. In contrast, when the flapper is made of a flexible material, a net fluid pumping is measured. We quantify the effectiveness of this pumping strategy and show that optimal pumping is achieved when the length of the flapper is on the same order as the elasto-hydrodynamic penetration length. We finally discuss the possible applications of flex...

  11. Passivity analysis for flexible multilink space manipulators

    Science.gov (United States)

    Damaren, Christopher J.

    1995-03-01

    The important input-output property of passivity is explored for a general flexible space manipulator with chain topology. The manipulator is assumed to consist of rigid and/or flexible links interconnected via revolute joints, and a free rigid spacecraft and cantilevered payload are modeled at the base and tip, respectively. Actuation on the spacecraft and torques at the joints serve as control inputs and a suitably modified input variable is constructed. The notion of reflected tip position introduced by Wang and Vidyasagar for a single flexible link is extended to the multilink case and used to define a corresponding modified output variable. The dynamics governing the system are developed using a Lagrangian approach and both linearized and nonlinear forms of the mapping relating modified inputs to modified outputs are examined. Our major result shows that the transfer function in the linear case is positive real when the spacecraft and payload are much more massive than the manipulator links. The corresponding nonlinear analysis shows that the mapping is, in fact, passive and uncovers an approximate static relationship between the elastic coordinates and applied torques. A numerical example employing the Space Shuttle, remote manipulator system, and payload is used to demonstrate the validity of the theoretical results. Applications to control system design are indicated.

  12. Granular materials interacting with thin flexible rods

    Science.gov (United States)

    Neto, Alfredo Gay; Campello, Eduardo M. B.

    2016-01-01

    In this work, we develop a computational model for the simulation of problems wherein granular materials interact with thin flexible rods. We treat granular materials as a collection of spherical particles following a discrete element method (DEM) approach, while flexible rods are described by a large deformation finite element (FEM) rod formulation. Grain-to-grain, grain-to-rod, and rod-to-rod contacts are fully permitted and resolved. A simple and efficient strategy is proposed for coupling the motion of the two types (discrete and continuum) of materials within an iterative time-stepping solution scheme. Implementation details are shown and discussed. Validity and applicability of the model are assessed by means of a few numerical examples. We believe that robust, efficiently coupled DEM-FEM schemes can be a useful tool to the simulation of problems wherein granular materials interact with thin flexible rods, such as (but not limited to) bombardment of grains on beam structures, flow of granular materials over surfaces covered by threads of hair in many biological processes, flow of grains through filters and strainers in various industrial segregation processes, and many others.

  13. Flexible manipulator control experiments and analysis

    Science.gov (United States)

    Yurkovich, S.; Ozguner, U.; Tzes, A.; Kotnik, P. T.

    1987-01-01

    Modeling and control design for flexible manipulators, both from an experimental and analytical viewpoint, are described. From the application perspective, an ongoing effort within the laboratory environment at the Ohio State University, where experimentation on a single link flexible arm is underway is described. Several unique features of this study are described here. First, the manipulator arm is slewed by a direct drive dc motor and has a rigid counterbalance appendage. Current experimentation is from two viewpoints: (1) rigid body slewing and vibration control via actuation with the hub motor, and (2) vibration suppression through the use of structure-mounted proof-mass actuation at the tip. Such an application to manipulator control is of interest particularly in design of space-based telerobotic control systems, but has received little attention to date. From an analytical viewpoint, parameter estimation techniques within the closed-loop for self-tuning adaptive control approaches are discussed. Also introduced is a control approach based on output feedback and frequency weighting to counteract effects of spillover in reduced-order model design. A model of the flexible manipulator based on experimental measurements is evaluated for such estimation and control approaches.

  14. An Efficient Method for Simulating Flexible Connectors

    Directory of Open Access Journals (Sweden)

    Frederick Li

    2009-04-01

    Full Text Available Physically-based dynamic simulation has been an active research area for many years. It is an important technique to enhance the realism of interactive multimedia or computer graphics applications. However, while most of these applications require real-time performance to handle user interactions, dynamic simulation is generally expensive computationally, as simulating a flexible (non-rigid object is typically done by modeling it with a large number of rigid bodies, in which constraints or impulses among the rigid bodies need to be processed continuously. In addition, in an interactive type of application, the motion of rigid bodies may sometimes be intervened. Such interactions, however, cannot be modeled with typical dynamic simulation techniques, such as associating constraints or behavior functions to the rigid bodies, due to its unpredictable nature. In this paper, we present a method for simulating flexible connectors. Our method combines dynamic constraints and impulse-based simulation to support real-time simulation of flexible connectors, such as ropes or chains. It also handles dynamic interventions efficiently.

  15. On the flexibility of phonetic categories

    Science.gov (United States)

    Francis, Alexander L.; Nusbaum, Howard C.

    2002-05-01

    Research on phonetic category acquisition suggests that over the course of development children shift the relative weight given to various acoustic cues to particular phonetic categories [S. Nittrouer, J. Phon. 20, 1-32 (1992)]. Adults also show flexibility in acquiring second language categories and learning to recognize synthetic speech. In both cases listeners must shift attention among some acoustic cues and change the weighting and mapping of others. Recent research demonstrated that category-level feedback can induce adult listeners to shift attention between conflicting cues to native stop-consonant categories. Subsequent research showed that adult listeners also reorganize the distribution of their attention to acoustic cues in learning to better understand synthetic speech. Moreover, research on learning foreign phonetic contrasts suggests that listeners can learn to direct attention to unfamiliar acoustic cues. These results suggest that phonetic categories are flexible in terms of the nature and quality of the acoustic cues that define them. We argue that this flexibility is a consequence of the basic nature of the general mechanism that mediates speech perception: In order to resolve the problem of context-conditioned variability, listeners must dynamically modify attention to the acoustic signal in a context-dependent manner.

  16. Flexible joints in structural and multibody dynamics

    Directory of Open Access Journals (Sweden)

    O. A. Bauchau

    2013-02-01

    Full Text Available Flexible joints, sometimes called bushing elements or force elements, are found in all structural and multibody dynamics codes. In their simplest form, flexible joints simply consist of sets of three linear and three torsional springs placed between two nodes of the model. For infinitesimal deformations, the selection of the lumped spring constants is an easy task, which can be based on a numerical simulation of the joint or on experimental measurements. If the joint undergoes finite deformations, identification of its stiffness characteristics is not so simple, specially if the joint is itself a complex system. When finite deformations occur, the definition of deformation measures becomes a critical issue. This paper proposes a family of tensorial deformation measures suitable for elastic bodies of finite dimension. These families are generated by two parameters that can be used to modify the constitutive behavior of the joint, while maintaining the tensorial nature of the deformation measures. Numerical results demonstrate the objectivity of the deformations measures, a feature that is not shared by the deformations measures presently used in the literature. The impact of the choice of the two parameters on the constitutive behavior of the flexible joint is also investigated.

  17. The Selective Autophagy Receptor p62 Forms a Flexible Filamentous Helical Scaffold

    Directory of Open Access Journals (Sweden)

    Rodolfo Ciuffa

    2015-05-01

    Full Text Available The scaffold protein p62/SQSTM1 is involved in protein turnover and signaling and is commonly found in dense protein bodies in eukaryotic cells. In autophagy, p62 acts as a selective autophagy receptor that recognizes and shuttles ubiquitinated proteins to the autophagosome for degradation. The structural organization of p62 in cellular bodies and the interplay of these assemblies with ubiquitin and the autophagic marker LC3 remain to be elucidated. Here, we present a cryo-EM structural analysis of p62. Together with structures of assemblies from the PB1 domain, we show that p62 is organized in flexible polymers with the PB1 domain constituting a helical scaffold. Filamentous p62 is capable of binding LC3 and addition of long ubiquitin chains induces disassembly and shortening of filaments. These studies explain how p62 assemblies provide a large molecular scaffold for the nascent autophagosome and reveal how they can bind ubiquitinated cargo.

  18. Monte Carlo simulations of flexible polyanions complexing with whey proteins at their isoelectric point

    Science.gov (United States)

    de Vries, R.

    2004-02-01

    Electrostatic complexation of flexible polyanions with the whey proteins α-lactalbumin and β-lactoglobulin is studied using Monte Carlo simulations. The proteins are considered at their respective isoelectric points. Discrete charges on the model polyelectrolytes and proteins interact through Debye-Hückel potentials. Protein excluded volume is taken into account through a coarse-grained model of the protein shape. Consistent with experimental results, it is found that α-lactalbumin complexes much more strongly than β-lactoglobulin. For α-lactalbumin, strong complexation is due to localized binding to a single large positive "charge patch," whereas for β-lactoglobulin, weak complexation is due to diffuse binding to multiple smaller charge patches.

  19. Applying flexible molecular docking to simulate protein retention behavior in hydrophobic interaction chromatography

    Institute of Scientific and Technical Information of China (English)

    ZHOU; Peng; TIAN; FeiFei; LI; ZhiLiang

    2007-01-01

    Interaction between proteins and stationary phase in hydrophobic interaction chromatography (HIC) is differentiated into two thermodynamic processes involving direct nonbonding/conformation interaction and surface hydrophobic effect of proteins, hence quantitatively giving rise to a binary linear relation between HIC retention time (RT) at concentrated salting liquid and ligand-protein binding free energy. Then, possible binding manners for 27 proteins of known crystal structures with hydrophobic ligands are simulated and analyzed via ICM flexible molecular docking and genetic algorithm, with results greatly consistent with experimental values. By investigation, it is confirmed local hydrophobic effects of proteins and nonbinding/conformation interaction between ligand and protein both notably influence HIC chromatogram retention behaviors, mainly focusing on exposed portions on the protein surface.

  20. Free-form Flexible Lithium-Ion Microbattery

    KAUST Repository

    Kutbee, Arwa

    2016-03-02

    Wearable electronics need miniaturized, safe and flexible power sources. Lithium ion battery is a strong candidate as high performance flexible battery. The development of flexible materials for battery electrodes suffers from the limited material choices. In this work, we present integration strategy to rationally design materials and processes to report flexible inorganic lithium-ion microbattery with no restrictions on the materials used. The battery shows an enhanced normalized capacity of 147 μAh/cm2 when bent.

  1. Towards Dynamic Camera Calibration for Constrained Flexible Mirror Imaging

    OpenAIRE

    Dunne, Aubrey K.; Mallon, John; Whelan, Paul F.

    2008-01-01

    Flexible mirror imaging systems consisting of a perspective camera viewing a scene reflected in a flexible mirror can provide direct control over image field-of-view and resolution. However, calibration of such systems is difficult due to the vast range of possible mirror shapes and the flexible nature of the system. This paper proposes the fundamentals of a dynamic calibration approach for flexible mirror imaging systems by examining the constrained case of single dimensional flexing. ...

  2. Technology Choice and Market Structure: Strategic Aspects of Flexible Manufacturing

    OpenAIRE

    Norman, George; THISSE, Jacques-François

    1998-01-01

    This paper shows that the adoption of flexible manufacturing techniques by firms leads to a tougher price regime. This need not benefit consumers since the tougher regime deters entry and facilitates segmented market structures. The ability of flexible manufacturing to deter entry is moderated by two forces: non-prohibitive costs of re-anchoring flexible manufacturing processes and entrants choosing to produce niche products using designated technologies rather than to adopt flexible manufact...

  3. 3D Position Tracking System for Flexible Cystoscopy

    OpenAIRE

    Munehiro Nakamura; Yusuke Kajiwara; Tatsuhito Hasegawa; Haruhiko Kimura

    2013-01-01

    Flexible cystoscopy is an examination that allows physicians to look inside the bladder. In flexible cystoscopy, beginner physicians tend to lose track of the observation due to complex handling patterns of a flexible cystoscope and poor characteristics of the bladder. In this paper, as a diagnostic support tool for beginner physicians in flexible cystoscopy, we propose a system for tracking the observation using cystoscopic images. Our system discriminates three handling patterns of a flexib...

  4. Rapid flexible docking using a stochastic rotamer library of ligands

    OpenAIRE

    Ding, Feng; Yin, Shuangye; Dokholyan, Nikolay V.

    2010-01-01

    Existing flexible docking approaches model the ligand and receptor flexibility either separately or in a loosely-coupled manner, which captures the conformational changes inefficiently. Here, we propose a flexible docking approach, MedusaDock, which models both ligand and receptor flexibility simultaneously with sets of discrete rotamers. We develop an algorithm to build the ligand rotamer library “on-the-fly” during docking simulations. MedusaDock benchmarks demonstrate a rapid sampling effi...

  5. Flexible employment as a unidirectional career? Results from field experiments

    OpenAIRE

    Boerner, Elisabeth; Boerner, Sabine

    2009-01-01

    Although the number of flexible workers is constantly growing, little is known about career paths built up on flexible employment. In this article, we investigate the chances of former flexible workers to be employed in a permanent full-time position. In two field experiments, we asked for employers evaluation of applicants with a flexible employment history. Results indicate that former part-time work is in fact perceived as a disadvantage for candidates when applying for a permanent full-t...

  6. Active vibration control techniques for flexible space structures

    Science.gov (United States)

    Parlos, Alexander G.; Jayasuriya, Suhada

    1990-01-01

    Two proposed control system design techniques for active vibration control in flexible space structures are detailed. Control issues relevant only to flexible-body dynamics are addressed, whereas no attempt was made to integrate the flexible and rigid-body spacecraft dynamics. Both of the proposed approaches revealed encouraging results; however, further investigation of the interaction of the flexible and rigid-body dynamics is warranted.

  7. Wage Flexibility and Economic Performance; Evidence Across Industrial Countries

    OpenAIRE

    Magda E. Kandil

    2000-01-01

    This paper provides new empirical evidence on the degree of nominal wage flexibility in a sample of nineteen industrial countries. Across countries, aggregate uncertainty increases the degree of wage flexibility in the face of various shocks. Wage flexibility stabilizes fluctuations in real output and guarantees workers a higher real standard of living in response to aggregate demand shocks. Wage flexibility in response to energy price shocks guarantees workers higher real wages without exace...

  8. MEASUREMENT AND DECOMPOSITION OF FLEXIBILITY OF MULTI-OUTPUT FIRMS

    OpenAIRE

    Renner, Swetlana; Glauben, Thomas; Hockmann, Heinrich

    2012-01-01

    Flexibility can be considered as a crucial factor of competitive advantage, especially under conditions of dynamically changing environments. Based on the classical microeconomic definition of flexibility, as introduced by Stigler, and some recent concepts developed in the production economics, this article proposes a primal flexibility measure for multi-product firms. When decomposed, this measure offers useful insights into possible sources of flexibility, especially by investigating the ro...

  9. Protein Localization with Flexible DNA or RNA

    DEFF Research Database (Denmark)

    Bernhardsson, Per Johan Sebastian; Mitarai, Namiko; Sneppen, Kim

    2012-01-01

    regions of the cell. However localization is at odds with the spreading of unbound molecules by diffusion. We model the cost and gain for specific enzyme activity using localization strategies based on binding to sites of intermediate specificity. While such bindings in themselves decrease the activity of......Localization of activity is ubiquitous in life, and also within sub-cellular compartments. Localization provides potential advantages as different proteins involved in the same cellular process may supplement each other on a fast timescale. It might also prevent proteins from being active in other...... the protein on its target site, they may increase protein activity if stochastic motion allows the acting protein to touch both the intermediate binding site and the specific site simultaneously. We discuss this strategy in view of recent suggestions on long non-coding RNA as a facilitator of...

  10. Protein localization with flexible DNA or RNA.

    Directory of Open Access Journals (Sweden)

    Sebastian Bernhardsson

    Full Text Available Localization of activity is ubiquitous in life, and also within sub-cellular compartments. Localization provides potential advantages as different proteins involved in the same cellular process may supplement each other on a fast timescale. It might also prevent proteins from being active in other regions of the cell. However localization is at odds with the spreading of unbound molecules by diffusion. We model the cost and gain for specific enzyme activity using localization strategies based on binding to sites of intermediate specificity. While such bindings in themselves decrease the activity of the protein on its target site, they may increase protein activity if stochastic motion allows the acting protein to touch both the intermediate binding site and the specific site simultaneously. We discuss this strategy in view of recent suggestions on long non-coding RNA as a facilitator of localized activity of chromatin modifiers.

  11. Protein localization with flexible DNA or RNA.

    Science.gov (United States)

    Bernhardsson, Sebastian; Mitarai, Namiko; Sneppen, Kim

    2012-01-01

    Localization of activity is ubiquitous in life, and also within sub-cellular compartments. Localization provides potential advantages as different proteins involved in the same cellular process may supplement each other on a fast timescale. It might also prevent proteins from being active in other regions of the cell. However localization is at odds with the spreading of unbound molecules by diffusion. We model the cost and gain for specific enzyme activity using localization strategies based on binding to sites of intermediate specificity. While such bindings in themselves decrease the activity of the protein on its target site, they may increase protein activity if stochastic motion allows the acting protein to touch both the intermediate binding site and the specific site simultaneously. We discuss this strategy in view of recent suggestions on long non-coding RNA as a facilitator of localized activity of chromatin modifiers. PMID:22347995

  12. Dynamics of a Highly Flexible Protein

    DEFF Research Database (Denmark)

    Andersen, Lisbeth

    Protein dynamics are important for protein function. Especially, dynamics plays an important role in ligand-binding where the induced fit and conformational selection binding mechanisms represent the extremes in a continuum of dynamic mechanisms. The difference between the mechanisms lies in the......-resolution structures of NCBD in complex with four binding partners are currently known and while three of them are practically identical and resemble the major conformation in the unbound ensemble, one shows NCBD adopting an alternative and markedly different conformation. The mechanisms underlying this conformational...... malleability are the subject of this defense. Using nuclear magnetic resonance (NMR) spectroscopy, the dynamics of NCBD have been investigated on timescales ranging from picoseconds to milliseconds using relaxation dispersion experiments, residual dipolar couplings and methyl group deuterium relaxation. From...

  13. APPLICATIONS OF RESEARCH TO THE PROBLEM OF INSTRUCTIONAL FLEXIBILITY.

    Science.gov (United States)

    SARTAIN, HARRY W.

    SELECTED RESEARCH ON THE PROBLEM OF INSTRUCTIONAL FLEXIBILITY IS SURVEYED AND DISCUSSED. BROAD TOPICS OF DISCUSSION ARE DEPARTMENTALIZATION, HOMOGENEOUS SECTIONING, INTERCLASS ABILITY SECTIONING, THE EXTENT OF VARIABILITY IN READING DEVELOPMENT, AND PRACTICES THAT MAY INCREASE FLEXIBILITY. AMONG THOSE PRACTICES TO INCREASE FLEXIBILITY ARE TEAM…

  14. Performance measurement of supply chain flexibility using witness

    Directory of Open Access Journals (Sweden)

    Rituraj Chandrakar

    2012-10-01

    Full Text Available In today’s global scenario of intense competition and environmental uncertainty flexibility in supply chain has an important role to play for the existence of any supply chain business. A need to be responsive to the constantly changing market scenario and cater to the customer needs, a certain degree of flexibility is required, which requires the coordination of many plants to produce and deliver goods to customers located in different places, and suppliers, which provide each plant with the required components. This paper intends to measure the degree of flexibility required for a two stage supply chain and assessing both the supplier flexibility and the assembler flexibility. In this paper, nine configurations of the SC are considered resulting from the combination of the three degrees of supplier and manufacturer flexibility, i.e. no flexibility, limited flexibility and total flexibility, respectively. Simulation model representing the different flexibility configurations are evaluated and the performance of each configuration analyzed to determine the flexibility configuration suitable to a supply chain. In particular the performance analysis of lead time, work-in-process, service level and cost are measured to determine the suitable flexibility.

  15. Micropatternable multifunctional nanocomposite polymers for flexible soft MEMS applications

    OpenAIRE

    Khosla, Ajit

    2011-01-01

    This thesis describes the fabrication, micro-machining, and characterization of novel multifunctional nanocomposite polymers for flexible microsystems.Various elastomers, primarily polydimethylsiloxane (PDMS), a silicone based elastomer, are chosen as substrate materials to create new flexible microsystems because of numerous benefits of PDMS including flexibility, biocompatibility, low cost, low toxicity, high oxidative and thermal stability, optical transparency, low permeability to water, ...

  16. 21 CFR 886.1390 - Flexible diagnostic Fresnel lens.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Flexible diagnostic Fresnel lens. 886.1390 Section... (CONTINUED) MEDICAL DEVICES OPHTHALMIC DEVICES Diagnostic Devices § 886.1390 Flexible diagnostic Fresnel lens. (a) Identification. A flexible diagnostic Fresnel lens is a device that is a very thin lens which...

  17. Flexible Training Strategy (National Task Force on Medical Staffing)

    OpenAIRE

    Department of Health

    2003-01-01

    Flexible Training Strategy (National Task Force on Medical Staffing) The Flexible Training Strategy, while endorsing flexible/part-time options recognises that the preferred option for the majority of doctors-in-training and consultants is most likely to continue to be full-time training and work. Click here to download PDF

  18. 5 CFR 532.513 - Flexible and compressed work schedules.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Flexible and compressed work schedules... REGULATIONS PREVAILING RATE SYSTEMS Premium Pay and Differentials § 532.513 Flexible and compressed work schedules. Federal Wage System employees who are authorized to work flexible and compressed work...

  19. 49 CFR 178.710 - Standards for flexible IBCs.

    Science.gov (United States)

    2010-10-01

    ... PACKAGINGS IBC Performance-Oriented Standards § 178.710 Standards for flexible IBCs. (a) The provisions of this section apply to flexible IBCs intended to contain solid hazardous materials. Flexible IBC types... body of the IBC or formed from a continuation of the IBC body material. (c) Construction...

  20. Operability and flexibility of a milk production line

    DEFF Research Database (Denmark)

    Cheng, Hongyuan; Friis, Alan

    2007-01-01

    parameters. The operability and flexibility of the nominal operating points were evaluated with a minimization of the process pasteurization and cooling temp. through vertex enumeration. The simulation procedure provides a quantitative method for developing a flexible process in dairy industry applications....... The established process operability and flexibility analysis framework and and flowsheet simulation can be adapted to other liquid food productions....