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Sample records for binary hammerhead ribozymes

  1. LNA nucleotides improve cleavage efficiency of singular and binary hammerhead ribozymes

    DEFF Research Database (Denmark)

    Christiansen, Janne K; Lobedanz, Sune; Arar, Khalil;

    2007-01-01

    concentrations, it was found that insertion of LNA monomers into the substrate binding arms allowed these to be shortened and results in a very active enzyme under both single and multiple turnover conditions. Incorporation of a mix of LNA and DNA residues further increased the multiple turnover cleavage...... activity. At high Mg(2+) concentrations or high substrate and ribozyme concentrations, the enhancing effect of LNA incorporation was even more prominent. Using LNA in the stem of Helix II diminished cleavage activity, but allowed deletion of the tetra-loop and thus separating the ribozyme into two...

  2. Kinetic characterization of two I/II format hammerhead ribozymes.

    OpenAIRE

    Clouet-d'Orval, B.; Uhlenbeck, O C

    1996-01-01

    Five new hammerhead ribozymes were designed that assemble through the formation of helices I and II (I/II format) instead of the more standard assembly through helices I and III (I/III format). The substrate binding and cleavage properties of such hammerheads could potentially be different due to the absence of loop II and the requirement for the entire catalytic core to assemble. Two I/II format hammerheads, HHalpha1 and HHalpha5, which show structural homogeneity on native gels, were charac...

  3. Ionic interactions and the global conformations of the hammerhead ribozyme

    DEFF Research Database (Denmark)

    Bassi, G S; Møllegaard, N E; Murchie, A I;

    1995-01-01

    Here we investigate the global conformation of the hammerhead ribozyme. Electrophoretic studies demonstrate that the structure is folded in response to the concentration and type of ions present. Folding based on colinear alignment of arms II and III is suggested, with a variable angle subtended ...

  4. Automated design of hammerhead ribozymes and validation by targeting the PABPN1 gene transcript.

    Science.gov (United States)

    Kharma, Nawwaf; Varin, Luc; Abu-Baker, Aida; Ouellet, Jonathan; Najeh, Sabrine; Ehdaeivand, Mohammad-Reza; Belmonte, Gabriel; Ambri, Anas; Rouleau, Guy; Perreault, Jonathan

    2016-02-29

    We present a new publicly accessible web-service, RiboSoft, which implements a comprehensive hammerhead ribozyme design procedure. It accepts as input a target sequence (and some design parameters) then generates a set of ranked hammerhead ribozymes, which target the input sequence. This paper describes the implemented procedure, which takes into consideration multiple objectives leading to a multi-objective ranking of the computer-generated ribozymes. Many ribozymes were assayed and validated, including four ribozymes targeting the transcript of a disease-causing gene (a mutant version of PABPN1). These four ribozymes were successfully tested in vitro and in vivo, for their ability to cleave the targeted transcript. The wet-lab positive results of the test are presented here demonstrating the real-world potential of both hammerhead ribozymes and RiboSoft. RiboSoft is freely available at the website http://ribosoft.fungalgenomics.ca/ribosoft/. PMID:26527730

  5. Experimental cancer gene therapy by multiple anti-survivin hammerhead ribozymes

    Institute of Scientific and Technical Information of China (English)

    Qi Fei; Yuwen Ke; Xuebiao Yao; Jingde Zhu; Hongyu Zhang; Lili Fu; Xinlan Dai; Baomei Gao; Min Ni; Chao Ge; Jinjun Li; Xia Ding

    2008-01-01

    To improve the efficacy of gene therapy for cancer, we designed four hammerhead ribozyme adenoviruses (R1 to R4) targeting the exposed regions of survivin mRNA. In addition to the in vitro characterization, which included a determination of the sequence specificity of cleavage by primer extension, assays for cell proliferation and for in vivo tumor growth were used to score for ribozyme efficiency.The resulting suppression of survivin expression induced mitotic catastrophe and cell death via the caspase-3-dependent pathway. Importantly, administration of the ribozyme adenoviruses inhibited tumor growth in a hepato-cellular carcinoma xenograft mouse model. Co-expression of R1, R3 and R4 ribozymes synergistically suppressed survivin and, as this combination targets all major forms of the survivin transcripts, produced the most potent anti-cancer effects. The adenoviruses carrying the multiple hammerhead ribozymes described in this report offered a robust gene therapy strategy against cancer.

  6. Capturing hammerhead ribozyme structures in action by modulating general base catalysis.

    Directory of Open Access Journals (Sweden)

    Young-In Chi

    2008-09-01

    Full Text Available We have obtained precatalytic (enzyme-substrate complex and postcatalytic (enzyme-product complex crystal structures of an active full-length hammerhead RNA that cleaves in the crystal. Using the natural satellite tobacco ringspot virus hammerhead RNA sequence, the self-cleavage reaction was modulated by substituting the general base of the ribozyme, G12, with A12, a purine variant with a much lower pKa that does not significantly perturb the ribozyme's atomic structure. The active, but slowly cleaving, ribozyme thus permitted isolation of enzyme-substrate and enzyme-product complexes without modifying the nucleophile or leaving group of the cleavage reaction, nor any other aspect of the substrate. The predissociation enzyme-product complex structure reveals RNA and metal ion interactions potentially relevant to transition-state stabilization that are absent in precatalytic structures.

  7. Expression and in vitro cleavage activity of anti-caspase-7 hammerhead ribozymes

    Institute of Scientific and Technical Information of China (English)

    Wei Zhang; Qing Xie; Xia-Qiu Zhou; Shan Jiang; You-Xin Jin

    2004-01-01

    AIM: To prepare hammerhead ribozymes against mouse caspase-7 and identify their cleavage activityin vitro, in order to select a ribozyme with specific cleavage activity against mouse caspase-7 as a potential gene therapy for apoptosis-related diseases.METHODS: Anti-caspase-7 ribozymes targeting sites 333and 394 (named Rz333 and Rz394) were designed by computer software, and their DNA sequences encoding ribozymes were synthesized. Caspase-7 DNA sequence was acquired by RT-PCR. Ribozymes and caspase-7 DNA obtained byin vitro transcription were cloned into pBSKneo U6' and pGEM-T vectors, respectively. The cleavage activity of ribozymes against mouse caspase-7 was identified by cleavage experimentsin vitro.RESULTS: Rz333 and Rz394 were designed and their DNA sequences were synthesized respectively. The expression vector of caspase-7 and plasmids containing Rz333 and Rz394 were reconstructed successfully. Ribozymes and caspase-7 mRNA were expressed byin vitro transcription.In vitro cleavage experiment showed that 243-nt and 744-nt segments were produced after caspase-7 mRNA was mixed with Rz333 in equivalent, and the cleavage efficiency was 67.98%. No cleaved segment was observed when caspase-7 mRNA was mixed with Rz394.CONCLUSION: Rz333 can site-specific cleave mouse caspase-7 mRNA, and it shows a potential for gene therapy of apoptosis-related diseases by down-regulating gene expression of caspase-7.

  8. Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell function

    Institute of Scientific and Technical Information of China (English)

    Run-Ping Gao; David R Brigstock

    2009-01-01

    AIM: To determine the effect of hammerhead ribozyme targeting connective tissue growth factor (CCN2) on human hepatic stellate cell (HSC) function. METHODS: CCN2 hammerhead ribozyme cDNA plus two self-cleaving sequences were inserted into pTriEx2 to produce pTriCCN2-Rz. Each vector was individually transfected into cultured LX-2 human HSCs, which were then stimulated by addition of transforming growth factor (TGF)-b1 to the culture medium. Semiquantitative RT-PCR was used to determine mRNA levels for CCN2 or collagen Ⅰ, while protein levels of each molecule in cell lysates and conditioned medium were measured by ELISA. Cell-cycle progression of the transfected cells was assessed by flow cytometry. RESULTS: In pTriEx2-transfected LX-2 cells, TGF-β1 treatment caused an increase in the mRNA level for CCN2 or collagen Ⅰ, and an increase in produced and secreted CCN2 or extracellular collagen Ⅰ protein levels. pTriCCN2-Rz-transfected LX-2 cells showed decreased basal CCN2 or collagen mRNA levels, as well as produced and secreted CCN2 or collagen Ⅰ protein. Furthermore, the TGF-b1-induced increase in mRNA or protein for CCN2 or collagen Ⅰ was inhibited partially in pTriCCN2-Rz-transfected LX-2 cells. Inhibition of CCN2 using hammerhead ribozyme cDNA resulted in fewer of the cells transitioning into S phase. CONCLUSION: Endogenous CCN2 is a mediator of basal or TGF-b1-induced collagen Ⅰ production in human HSCs and regulates entry of the cells into Sphase.

  9. Fast and simple purification of chemically modified hammerhead ribozymes using a lipophilic capture tag.

    OpenAIRE

    Sproat, B S; Rupp, T; Menhardt, N; Keane, D.; Beijer, B

    1999-01-01

    A new type of 5'-lipophilic capture tag is described, enabling the facile reverse phase HPLC purification of chemically modified hammerhead ribozymes (oligozymes) whilst still carrying the 2'-O-tert.-butyldimethylsilyl protection of the essential riboses. In its most convenient form, the capture tag consists of a simple diol, such as hexan-1,6-diol, which at one end is attached via a silyl residue to a highly lipophilic entity such as tocopherol (vitamin E) or cholesterol, and the other end i...

  10. Targeted cleavage of hepatitis E virus 3' end RNA mediated by hammerhead ribozymes inhibits viral RNA replication

    International Nuclear Information System (INIS)

    The 3' end of hepatitis E virus (HEV) contains cis-acting regulatory element, which plays an important role in viral replication. To develop specific replication inhibitor at the molecular level, mono- and di-hammerhead ribozymes (Rz) were designed and synthesized against the conserved 3' end sequences of HEV, which cleave at nucleotide positions 7125 and 7112/7125, respectively. Di-hammerhead ribozyme with two catalytic motifs in tandem was designed to cleave simultaneously at two sites spaced 13 nucleotides apart, which increases the overall cleavage efficiency and prevents the development of escape mutants. Specific cleavage products were obtained with both the ribozymes in vitro at physiological conditions. The inactive control ribozymes showed no cleavage. The ribozymes showed specific inhibition of HEV 3' end fused-luciferase reporter gene expression by ∼37 and ∼60%, respectively in HepG2 cells. These results demonstrate a feasible approach to inhibit the HEV replication to a limited extent by targeting the cis-acting 3' end of HEV with hammerhead ribozymes

  11. Activity identification of chimeric anti-caspase-3 mRNA hammerhead ribozyme in vitro and in vivo

    Institute of Scientific and Technical Information of China (English)

    XU; Renhuan; (

    2001-01-01

    [1]Andrew, F., Gerard, E., A license to kill, Cell, 1996, 85: 781-784.[2]Thornberry, N. A., Lazebnik, Y., Caspases: Enemies within, Science, 1998, 281: 1312-1316.[3]Kijima, H., Ishida, H., Ohkawa, T. et al., Therapeutic application of ribozymes, Pharmacol. Ther., 1995, 68: 247-264.[4]Phylactou, L. A., Kilpatrick, M. W., Wood, M. J., Ribozymes as therapeutic tools for genetic disease, Hum. Mol. Genet., 1998, 7(10): 1649-1653.[5]Bettrand, E., Pictet, R ., Grange, T., Can heamerhead ribozymes be efficient tools inactivate gene function? Nucleic Acids Res., 1994, 22: 293-300.[6]Lieber, A., Strauss, M., Selection of efficient cleavage sites in target RNAs by using a ribozyme expression library, Mol. Cell Biol., 1995, 15: 540-551.[7]Xu, R. H., Zhou, X. Q., Xie, Q. et al., Preparation and identification of hammerhead ribozyme in vitro against rat caspase-3 mRNA fragment, Chin. J. Hepatol., 2000,8: 361-363.[8]Liu, J., Jin, Y. X., Wang, D. B., A novel vector for abundant expression of antisense RNA, triplex-forming RNA and ribozyme in vivo, High Technology Letters, 2000, 6: 84-88.[9]Sambrook, J., Fritsch, E. F., Maniatis, T., Molecular Cloning: A Laboratory Manual, 2nd ed., New York: Cold Spring Harbor Laboratory Press, 1989.[10]Porter, A. G., J?nicke, R. U., Emerging roles of caspase-3 in apoptosis, Cell Death Differ, 1999, 6: 99-104.[11]Cryns, V., Yuan, J., Proteases to die for, Genes Dev., 1998, 12: 1551-1570.[12]Narendra, K. V., Anikumar, R. K., Fritz, E., Recent developments in the hammerhead ribozyme field, Nucleic Acids Research, 1998, 26: 5237-5242.

  12. Preparation of anti-mouse caspase-12 mRNA hammerhead ribozyme and identification of its activity in vitro

    Institute of Scientific and Technical Information of China (English)

    Shan Jiang; Qing Xie; Wei Zhang; Xia-Qiu Zhou; You-Xin Jin

    2005-01-01

    AIM: To prepare and identify specific anti-mouse caspase12 hammerhead ribozymes in vitro, in order to select a more effective ribozyme against mouse caspase-12 as a potential tool to rescue cells from endoplasmic reticulum stress induced apoptosis.METHODS: Two hammerhead ribozymes directed separately against 138 and 218 site of nucleotide of mouse caspase-12 mRNA were designed by computer software,and their DNA sequences were synthesized. The synthesized ribozymes were cloned into an eukaryotic expression vector-neorpBSKU6 and embedded in U6 SnRNA context for further study. Mouse caspase-12 gene segment was cloned into PGEM-T vector under the control of T7 RNA polymerase promoter (containing gene sequence from positions nt 41 to nt 894) as target. In vitro transcription both the ribozymes and target utilize T7 promoter. The target was labeled with [α-32P]UTP, while ribozymes were not labeled. After gel purification the RNAs were dissolved in RNase free water. Ribozyme and target were incubated for 90 min at 37 ℃ in reaction buffer (40 mmol/L Tris-HCL,pH 7.5, 10 mmol/L Mg2+). Molar ratio of ribozyme vs target was 30:1. Samples were analyzed on 6% PAGE (containing 8 mol/L urea).RESULTS: Both caspase-12 and ribozyme gene sequences were successfully cloned into expression vector confirmed by sequencing. Ribozymes and caspase-12 mRNA were obtained by in vitro transcription. Cleavage experiment showed that in a physiological similar condition (37 ℃,pH 7.5), Rz138 and Rz218 both cleaved targets at predicted sites, for Rz138 the cleavage efficiency was about 100%,for Rz218 the value was 36.66%.CONCLUSION: Rz138 prepared in vitro can site specific cleave mouse caspase-12 mRNA with an excellent efficiency. It shows a potential to suppress the expression of caspase-12 in vivo, thus provided a new way to protect cells from ER stress induced apoptosis.

  13. EXPLORATION OF SCHISTOSOMA MANSONI HAMMERHEAD RIBOZYME CATALYSIS AND STRUCTURE: TOWARDS DIRECT OBSERVATION OF CLEAVAGE AND LIGATION, AND A 1.55 Å FULL-LENGTH MG2+-BOUND CRYSTAL STRUCTURE

    OpenAIRE

    Anderson, Michael

    2012-01-01

    As a relatively simple and well-studied molecule, the hammerhead ribozyme is an ideal system to study RNA catalysis and structure. A deeper understanding of the hammerhead catalytic mechanism and the role of divalent ions in catalysis lends support to the exploration of more complex RNA machinery such as the ribosome, and ultimately may assist in the design of new medical therapies. Kinetic study of the hammerhead ribozyme to date has largely derived from bulk assays monitoring millions of ...

  14. Inhibition of Hepatitis B Virus Replication and Expression in Vitro and in Vivo by the Hammerhead Ribozymes Targeted Different Sites

    Institute of Scientific and Technical Information of China (English)

    Wei; Dai; Rong; Zhou; Hong; Yu; Xiao-juan; Li

    2012-01-01

    Objective To develop an effective and specific medicine targeting hepatitis B virus(HBV) pregenome. Based on the identified accessible target sites for hammerhead ribozyme in our previous researches, a recombinant hepatitis delta virus(HDV) ribozyme was chosen and used to demonstrate the effective cleavage in vitro and in vivo. Methods Three hammerhead ribozymes for potential target sites(S, X and C genes) and co-expression plasmid(pTr-dB, pTdδ-dB, pTrX-dB and pTrC-dB) as well as four HDV-ribozyme chimera constructs with HBV(pTdXX, pTdXC, pTdSX and pTdSC) were severally chosen to validate the inhibition of the replication and expression of HBV. The co-expression plasmids(pTdδ and pTr-Db) in physiological saline were hydrodynamically injected to mice by tail vein. Results Compared with the group injected with pTr-dB in Huh-7 cell, hepatitis B surface antigen(HBsAg) was reduced by 31% in the group injected with pTdδ-dB, by 54%, 26%, 72% and 97% in the group injected with recombinant-ribozymes pTdSX, pTdSC, pTdXC and pTdXX, respectively. The inhibiting effects of endogenous ribozymes RzX and RzC on the HBsAg expression were 66% and 57%, respectively. Compared with the positive control, the amount of HBsAg was decreased in mice injected with pTdXX through tail vein by 88% and 96% on the second day and the third day, respectively. HBsAg was undetectable on the 6th day and could not primitively be detected on the 9th day in the sera from all mice. HBV DNA was not detected in the sera of BALB/c mice injected with pTdXX-dB, pTrX-dB or replicating-defective plasmid pHBV, while HBV DNA replication in control group could be detected on the 6th day. While HBcAg could not be detected in liver tissues of mice injected with plasmid pTdXX-dB on the 3rd day. Conclusions Encoding regions of HBV S, C and X gene were the effective cleavage sites for hammerhead ribozyme in vitro and in vivo, which provides basis for further construction of therapeutic recombinant HDV and the

  15. Antiviral Hammerhead Ribozymes Are Effective for Developing Transgenic Suppression of Chikungunya Virus in Aedes aegypti Mosquitoes.

    Science.gov (United States)

    Mishra, Priya; Furey, Colleen; Balaraman, Velmurugan; Fraser, Malcolm J

    2016-01-01

    The chikungunya virus (CHIKV) is an emerging pathogen with widespread distribution in regions of Africa, India, and Asia that threatens to spread into temperate climates with the introduction of its major vector, Aedes albopictus. CHIKV causes a disease frequently misdiagnosed as dengue fever, with potentially life-threatening symptoms that can result in a longer-term debilitating arthritis. The increasing risk of spread from endemic regions via human travel and commerce and the current absence of a vaccine put a significant proportion of the world population at risk for this disease. In this study we designed and tested hammerhead ribozymes (hRzs) targeting CHIKV structural protein genes of the RNA genome as potential antivirals both at the cellular and in vivo level. We employed the CHIKV strain 181/25, which exhibits similar infectivity rates in both Vero cell cultures and mosquitoes. Virus suppression assay performed on transformed Vero cell clones of all seven hRzs demonstrated that all are effective at inhibiting CHIKV in Vero cells, with hRz #9 and #14 being the most effective. piggyBac transformation vectors were constructed using the Ae. aegypti t-RNA(val) Pol III promoted hRz #9 and #14 effector genes to establish a total of nine unique transgenic Higgs White Eye (HWE) Ae. aegypti lines. Following confirmation of transgene expression by real-time polymerase chain reaction (RT-PCR), comparative TCID50-IFA analysis, in situ Immuno-fluorescent Assays (IFA) and analysis of salivary CHIKV titers demonstrated effective suppression of virus replication at 7 dpi in heterozygous females of each of these transgenic lines compared with control HWE mosquitoes. This report provides a proof that appropriately engineered hRzs are powerful antiviral effector genes suitable for population replacement strategies. PMID:27294950

  16. Mechanistic studies of copper(II)-aminoglycoside mediated DNA damage and magnesium catalyzed nuclease activity of hammerhead ribozyme

    Science.gov (United States)

    Patwardhan, Anjali A.

    The antibacterial activity of aminoglycosides stems from their high affinity binding to the 16S rRNA in bacteria resulting in inhibition of protein synthesis. Used to treat acute bacterial infections these antibiotics have limited applications due to their high dosage requirements and the emergence of resistant strains. We have synthesized and characterized Cu(II) derivatives of the aminoglycosides, kanamycin A, tobramycin, neamine, kanamycin B, neomycin B, and paromomycin. The first three exhibit preferential and tight binding to Cu(II) as against neomycin B and kanamycin B and paromomycin. EPR of frozen solutions and UV-visible spectroscopy suggest a change in geometry around the Cu(II) but the stabilities of the complexes in water differ. These copper derivatives efficiently cleave plasmid DNA at micromolar concentrations (hydrolytic) and at nanomolar concentrations in the presence co-reactants like hydrogen peroxide or ascorbic acid. Hydrolysis is multi turnover and exhibits Michelis-Menten kinetics with enzyme-like behavior whereas oxidative cleavage is highly specific with C-4' H abstraction resulting in characteristic base propenal and nucleotide base products. Hydroxyl radicals generated are copper based and are generated in close proximity of the substrate. Hammerhead ribozymes are selectively hydrolyzed in the presence of divalent ions with Mg2+ being the metal ion of choice in vivo . Our studies with complex ions like cobalt hexaammine and fac-triamminetriaquochromium(III) establish outer sphere interactions of Mg2+ with the hammerhead in the catalytic site. There are two sets of sites, one structural and one catalytic. Complex ions in the catalytic site and divalent ions in the structural site result in a slow but active hammerhead ribozyme suggesting that the complex ions are not inhibitory, contrary to what was suggested previously.

  17. Phylogenetic footprinting of non-coding RNA: hammerhead ribozyme sequences in a satellite DNA family of Dolichopoda cave crickets (Orthoptera, Rhaphidophoridae

    Directory of Open Access Journals (Sweden)

    Venanzetti Federica

    2010-01-01

    Full Text Available Abstract Background The great variety in sequence, length, complexity, and abundance of satellite DNA has made it difficult to ascribe any function to this genome component. Recent studies have shown that satellite DNA can be transcribed and be involved in regulation of chromatin structure and gene expression. Some satellite DNAs, such as the pDo500 sequence family in Dolichopoda cave crickets, have a catalytic hammerhead (HH ribozyme structure and activity embedded within each repeat. Results We assessed the phylogenetic footprints of the HH ribozyme within the pDo500 sequences from 38 different populations representing 12 species of Dolichopoda. The HH region was significantly more conserved than the non-hammerhead (NHH region of the pDo500 repeat. In addition, stems were more conserved than loops. In stems, several compensatory mutations were detected that maintain base pairing. The core region of the HH ribozyme was affected by very few nucleotide substitutions and the cleavage position was altered only once among 198 sequences. RNA folding of the HH sequences revealed that a potentially active HH ribozyme can be found in most of the Dolichopoda populations and species. Conclusions The phylogenetic footprints suggest that the HH region of the pDo500 sequence family is selected for function in Dolichopoda cave crickets. However, the functional role of HH ribozymes in eukaryotic organisms is unclear. The possible functions have been related to trans cleavage of an RNA target by a ribonucleoprotein and regulation of gene expression. Whether the HH ribozyme in Dolichopoda is involved in similar functions remains to be investigated. Future studies need to demonstrate how the observed nucleotide changes and evolutionary constraint have affected the catalytic efficiency of the hammerhead.

  18. Phylogenetic footprinting of non-coding RNA: hammerhead ribozyme sequences in a satellite DNA family of Dolichopoda cave crickets (Orthoptera, Rhaphidophoridae)

    OpenAIRE

    Venanzetti Federica; Johnsen Arild; Martinsen Lene; Bachmann Lutz

    2010-01-01

    Abstract Background The great variety in sequence, length, complexity, and abundance of satellite DNA has made it difficult to ascribe any function to this genome component. Recent studies have shown that satellite DNA can be transcribed and be involved in regulation of chromatin structure and gene expression. Some satellite DNAs, such as the pDo500 sequence family in Dolichopoda cave crickets, have a catalytic hammerhead (HH) ribozyme structure and activity embedded within each repeat. Resul...

  19. Zinc-dependent cleavage in the catalytic core of the hammerhead ribozyme: evidence for a pH-dependent conformational change

    OpenAIRE

    Borda, Emily J.; Markley, John C.; Sigurdsson, Snorri Th.

    2003-01-01

    We have characterized a novel Zn2+-catalyzed cleavage site between nucleotides C3 and U4 in the catalytic core of the hammerhead ribozyme. In contrast to previously described divalent metal-ion-dependent cleavage of RNA, U4 cleavage is only observed in the presence of Zn2+. This new cleavage site has an unusual pH dependence, in that U4 cleavage products are only observed above pH 7.9 and reach a maximum yield at about pH 8.5. These data, together with the fact that no metal ion-binding site ...

  20. Secondary structure in solution of two anti-HIV-1 hammerhead ribozymes as investigated by two-dimensional 1H 500 MHz NMR spectroscopy in water

    Science.gov (United States)

    Sarma, R. H.; Sarma, M. H.; Rein, R.; Shibata, M.; Setlik, R. S.; Ornstein, R. L.; Kazim, A. L.; Cairo, A.; Tomasi, T. B.

    1995-01-01

    Two hammerhead chimeric RNA/DNA ribozymes (HRz) were synthesized in pure form. Both were 30 nucleotides long, and the sequences were such that they could be targeted to cleave the HIV-1 gag RNA. Named HRz-W and HRz-M, the former had its invariable core region conserved, the latter had a uridine in the invariable region replaced by a guanine. Their secodary structures were determined by 2D NOESY 1H 500 MHz NMR spectroscopy in 90% water and 10% D2(0), following the imino protons. The data show that both HRz-M and HRz-W form identical secondary structures with stem regions consisting of continuous stacks of AT and GT pairs. An energy minimized computer model of this stem region is provided. The results suggest that the loss of catalytic activity that is known to result when an invariant core residue is replaced is not related to the secondary structure of the ribozymes in the absence of substrate.

  1. Spiegelzymes® mirror-image hammerhead ribozymes and mirror-image DNAzymes, an alternative to siRNAs and microRNAs to cleave mRNAs in vivo?

    Directory of Open Access Journals (Sweden)

    Eliza Wyszko

    Full Text Available With the discovery of small non-coding RNA (ncRNA molecules as regulators for cellular processes, it became intriguing to develop technologies by which these regulators can be applied in molecular biology and molecular medicine. The application of ncRNAs has significantly increased our knowledge about the regulation and functions of a number of proteins in the cell. It is surprising that similar successes in applying these small ncRNAs in biotechnology and molecular medicine have so far been very limited. The reasons for these observations may lie in the high complexity in which these RNA regulators function in the cells and problems with their delivery, stability and specificity. Recently, we have described mirror-image hammerhead ribozymes and DNAzymes (Spiegelzymes® which can sequence-specifically hydrolyse mirror-image nucleic acids, such as our mirror-image aptamers (Spiegelmers discovered earlier. In this paper, we show for the first time that Spiegelzymes are capable of recognising complementary enantiomeric substrates (D-nucleic acids, and that they efficiently hydrolyse them at submillimolar magnesium concentrations and at physiologically relevant conditions. The Spiegelzymes are very stable in human sera, and do not require any protein factors for their function. They have the additional advantages of being non-toxic and non-immunogenic. The Spiegelzymes can be used for RNA silencing and also as therapeutic and diagnostic tools in medicine. We performed extensive three-dimensional molecular modelling experiments with mirror-image hammerhead ribozymes and DNAzymes interacting with D-RNA targets. We propose a model in which L/D-double helix structures can be formed by natural Watson-Crick base pairs, but where the nucleosides of one of the two strands will occur in an anticlinal conformation. Interestingly enough, the duplexes (L-RNA/D-RNA and L-DNA/D-RNA in these models can show either right- or left-handedness. This is a very new

  2. The effect of structure in a long target RNA on ribozyme cleavage efficiency.

    OpenAIRE

    Campbell, T B; McDonald, C K; Hagen, M.

    1997-01-01

    Inhibition of gene expression by catalytic RNA (ribozymes) requires that ribozymes efficiently cleave specific sites within large target RNAs. However, the cleavage of long target RNAs by ribozymes is much less efficient than cleavage of short oligonucleotide substrates because of higher order structure in the long target RNA. To further study the effects of long target RNA structure on ribozyme cleavage efficiency, we determined the accessibility of seven hammerhead ribozyme cleavage sites i...

  3. Engineering a ribozyme cleavage-induced split fluorescent aptamer complementation assay

    OpenAIRE

    Ausländer, Simon; Fuchs, David; Hürlemann, Samuel; Ausländer, David; Fussenegger, Martin

    2016-01-01

    Hammerhead ribozymes are self-cleaving RNA molecules capable of regulating gene expression in living cells. Their cleavage performance is strongly influenced by intra-molecular loop–loop interactions, a feature not readily accessible through modern prediction algorithms. Ribozyme engineering and efficient implementation of ribozyme-based genetic switches requires detailed knowledge of individual self-cleavage performances. By rational design, we devised fluorescent aptamer-ribozyme RNA archit...

  4. Structure of an anti-HIV-1 hammerhead ribozyme complex with a 17-mer DNA substrate analog of HIV-1 gag RNA and a mechanism for the cleavage reaction: 750 MHz NMR and computer experiments

    Science.gov (United States)

    Ojha, R. P.; Dhingra, M. M.; Sarma, M. H.; Myer, Y. P.; Setlik, R. F.; Shibata, M.; Kazim, A. L.; Ornstein, R. L.; Rein, R.; Turner, C. J.; Sarma, R. H.

    1997-01-01

    The structure of an anti-HIV-1 ribozyme-DNA abortive substrate complex was investigated by 750 MHz NMR and computer modeling experiments. The ribozyme was a chimeric molecule with 30 residues-18 DNA nucleotides, and 12 RNA residues in the conserved core. The DNA substrate analog had 17 residues. The chimeric ribozyme and the DNA substrate formed a shortened ribozyme-abortive substrate complex of 47 nucleotides with two DNA stems (stems I and III) and a loop consisting of the conserved core residues. Circular dichroism spectra showed that the DNA stems assume A-family conformation at the NMR concentration and a temperature of 15 degrees C, contrary to the conventional wisdom that DNA duplexes in aqueous solution populate entirely in the B-form. It is proposed that the A-family RNA residues at the core expand the A-family initiated at the core into the DNA stems because of the large free energy requirement for the formation of A/B junctions. Assignments of the base H8/H6 protons and H1' of the 47 residues were made by a NOESY walk. In addition to the methyl groups of all T's, the imino resonances of stems I and III and AH2's were assigned from appropriate NOESY walks. The extracted NMR data along with available crystallographic data, were used to derive a structural model of the complex. Stems I and III of the final model displayed a remarkable similarity to the A form of DNA; in stem III, a GC base pair was found to be moving into the floor of the minor groove defined by flanking AT pairs; data suggest the formation of a buckled rhombic structure with the adjacent pair; in addition, the base pair at the interface of stem III and the loop region displayed deformed geometry. The loop with the catalytic core, and the immediate region of the stems displayed conformational multiplicity within the NMR time scale. A catalytic mechanism for ribozyme action based on the derived structure, and consistent with biochemical data in the literature, is proposed. The complex

  5. Ultrasensitive monitoring of ribozyme cleavage product using molecular-beacon-ligation system

    Institute of Scientific and Technical Information of China (English)

    MENG XiangXian; TANG ZhiWen; WANG KeMin; TAN WeiHong; YANG XiaoHai; LI Jun; GUO QiuPing

    2007-01-01

    This paper reports a new approach to detect ribozyme cleavage product based on the molecular- beacon-ligation system. The molecular beacon, designed in such a way that one-half of its loop is complementary to ribozyme cleavage product, is used to monitor ligation process of RNA/DNA complex in a homogeneous solution and to convert directly cleavage product information into fluorescence signal. The method need not label ribozyme and ribozyme substrate, which is fast, simple and ultrasensitive for detection of cleavage product. Detection limit of the assay is 0.05 nmol/L. The cleavage product of hammerhead ribozyme against hepatitis C virus RNA (HCV-RNA) was detected perfectly based on this assay. Owing to its ultrasensitivity, excellent specificity, convenience and fidelity, this method might hold out great promise in ribozyme reaction and ribozyme gene therapy.

  6. Construction of Multi-ribozyme Expression System and Its Characterization of Cleavage on the MDR1/MRP1 Double Target Substrate in vitro

    Institute of Scientific and Technical Information of China (English)

    TIAN Sheng-li; ZHENG Suo; LIU Shi-de; ZHANG Jian-hua; XU Dong-ping; OHNUMA Takao

    2009-01-01

    To improve catalytic activity of ribozyme on its substrate,the multi-ribozyme expression system was designed and constructed from 20 cis-acting hammerhead ribozymes undergoing self-cleavage with 10 trans-acting hammerhead ribozymes inserted altematively regularly and the plasmid of pGEM-MDRI/MRPI used to transcribe the M DRI/MRPI(196/210) substrate containing double target sites was also constructed by DNA recombination.Endonuclease digestion analysis and DNA sequencing indicate all the recombinant plasmids were correct.The cleavage activities were evaluated for the multi-ribozyme expression system on the MDR1/MRP1 substrate in the cell free system.The results demonstrate that the cis-acting hammerhead ribozymes in the multi-ribozyme expression system were able to cleave themselves and the 72 nt of 196Rz and the 71 nt of 210Rz trans-acting hammerhead ribozymes were liberated effectively,and the trans-acting hammerhead ribozymes released were able to act on the MDR1/MRP1 double target RNA substrate and cleave the target RNA at specific sites effectively.The multiribozyme expression system of the [Coat'A196Rz/Coat'B210Rz]5 is more significantly superior to that of the [Coat'A 196Rz/Coat'B210Rz]1 in cleavage of RNA substrate.The fractions cleaved by [Coat'A196Rz/Coat'B210Rz]5 on the MDR1/MRP1 substrate for 8 h at observed temperatures showed no marked difference.The studies of Mg2+ on cleavage efficiency indicate that cleavage reaction is dependent on Mg2+ ions concentration.The plot of Ig(kobs) vs.Igc(Mg2+) displays a linear relationship between 2.5 mmol/L and 20 mmol/L Mg2..It suggests that Mg2+ ions play a crucial role in multi-ribozyme cleavage on the substrate.

  7. Ribozyme-mediated reversal of the multidrug-resistant phenotype.

    OpenAIRE

    Scanlon, K J; Ishida, H.; Kashani-Sabet, M

    1994-01-01

    This study examined the effects of suppressing c-fos oncogene expression on multidrug resistance (MDR). A2780S human ovarian carcinoma cells with resistance to actinomycin D were isolated and the resultant A2780AD cells exhibited the MDR phenotype. A hammerhead ribozyme designed to cleave fos RNA cloned into the pMAMneo plasmid was transfected into A2780AD cells. Induction of the ribozyme resulted in decreased expression of c-fos, as well as that of the MDR gene (mdr-1), c-jun, and mutant p53...

  8. 碱基切除修复基因HOGG1特异性锤头状核酶表达载体的构建及其功能的初步研究%Constructing the Eukaryotic Expression Vector to Study Preliminarily the Functions of Hammerhead Ribozyme Targeting Base Excision Repair Gene HOGG1

    Institute of Scientific and Technical Information of China (English)

    张遵真; 张勤; 吴媚

    2006-01-01

    Objective Adriamycin is widely used as an effective anti-tumor drug clinically treating a number of human cancers, but the effect of adriamycin is limited by drug resistance. The various kinds of investigations indicated that the anti-tumor activity of adriamycin resulted from drug-induced free radical formation. The free radicals could lead to oxidative DNA damage, and the lesion would be repaired by base excision repair (BER) pathway. Human 8-oxoguanine DNA glycosylase 1 (HOGG1) is a key enzyme on BER pathway. To study the influence and biological mechanism of the HOGG1 to adriamycin drug-sensitivity, the eukaryotic expression vector with gene of hammerhead ribozyme targeting HOGG1 mRNA would be constructed and identified, and then the change of drug-sensitivity in lung cancer A549 cells would be investigated. Methods According to computer design, two specific restriction site BamHⅠ and EcoRⅠ were added to both ends of the ribozyme gene, then the modified ribozyme gene was synthesized and cloned into the eukaryotic expression vector pcDNA3.1(+). The positive recombinants were screened by ampicillin resistance, and plasmids were extracted from the positive recombinants and digested by BamH Ⅰ and EcoR Ⅰ, and then were analyzed by agarose gel electrophoresis and DNA sequencing. The recombinants were transiently transfected into A549 cells. The positive recombinants were identified by reverse transcription-polymerase chain reaction (RT-PCR) targeting to NEO gene, which was a neomycin resistance gene for selection of stable cell lines and only existed in vectors. The changes of HOGG1 mRNA in A549 cells were detected by RT-PCR. Then the cellular sensitivity to adriamycin was tested by comparison between untransfected cells and transfected cells by MTT assay. The adriamycin-induced DNA damage was investigated by comet assay or single cell gel electrophoresis (SCGE) between untransfected and transfected cells. Results The recombinants containing the ribozyme gene

  9. Group I intron ribozymes

    DEFF Research Database (Denmark)

    Nielsen, Henrik

    2012-01-01

    Group I intron ribozymes constitute one of the main classes of ribozymes and have been a particularly important model in the discovery of key concepts in RNA biology as well as in the development of new methods. Compared to other ribozyme classes, group I intron ribozymes display considerable...... intronic products of these pathways have the potential to integrate into targets and to form various types of circular RNA molecules. Thus, group I intron ribozymes and associated elements found within group I introns is a rich source of biological phenomena. This chapter provides a strategy and protocols...... for initial characterization of new group I intron ribozymes....

  10. Kinetics of hairpin ribozyme cleavage in yeast.

    OpenAIRE

    Donahue, C P; Fedor, M J

    1997-01-01

    Hairpin ribozymes catalyze a self-cleavage reaction that provides a simple model for quantitative analyses of intracellular mechanisms of RNA catalysis. Decay rates of chimeric mRNAs containing self-cleaving ribozymes give a direct measure of intracellular cleavage kinetics in yeast. Intracellular ribozyme-mediated cleavage occurs at similar rates and shows similar inhibition by ribozyme mutations as ribozyme-mediated reactions in vitro, but only when ribozymes are located in a favorable mRNA...

  11. Ribozyme-based aminoglycoside switches of gene expression engineered by genetic selection in S. cerevisiae.

    Science.gov (United States)

    Klauser, Benedikt; Atanasov, Janina; Siewert, Lena K; Hartig, Jörg S

    2015-05-15

    Systems for conditional gene expression are powerful tools in basic research as well as in biotechnology. For future applications, it is of great importance to engineer orthogonal genetic switches that function reliably in diverse contexts. RNA-based switches have the advantage that effector molecules interact immediately with regulatory modules inserted into the target RNAs, getting rid of the need of transcription factors usually mediating genetic control. Artificial riboswitches are characterized by their simplicity and small size accompanied by a high degree of modularity. We have recently reported a series of hammerhead ribozyme-based artificial riboswitches that allow for post-transcriptional regulation of gene expression via switching mRNA, tRNA, or rRNA functions. A more widespread application was so far hampered by moderate switching performances and a limited set of effector molecules available. Here, we report the re-engineering of hammerhead ribozymes in order to respond efficiently to aminoglycoside antibiotics. We first established an in vivo selection protocol in Saccharomyces cerevisiae that enabled us to search large sequence spaces for optimized switches. We then envisioned and characterized a novel strategy of attaching the aptamer to the ribozyme catalytic core, increasing the design options for rendering the ribozyme ligand-dependent. These innovations enabled the development of neomycin-dependent RNA modules that switch gene expression up to 25-fold. The presented aminoglycoside-responsive riboswitches belong to the best-performing RNA-based genetic regulators reported so far. The developed in vivo selection protocol should allow for sampling of large sequence spaces for engineering of further optimized riboswitches. PMID:24871672

  12. RiboSubstrates: a web application addressing the cleavage specificities of ribozymes in designated genomes

    Directory of Open Access Journals (Sweden)

    Bergeron Lucien

    2006-10-01

    Full Text Available Abstract Background RNA-dependent gene silencing is becoming a routine tool used in laboratories worldwide. One of the important remaining hurdles in the selection of the target sequence, if not the most important one, is the designing of tools that have minimal off-target effects (i.e. cleaves only the desired sequence. Increasingly, in the current dawn of the post-genomic era, there is a heavy reliance on tools that are suitable for high-throughput functional genomics, consequently more and more bioinformatic software is becoming available. However, to date none have been designed to satisfy the ever-increasing need for the accurate selection of targets for a specific silencing reagent. Results In order to overcome this hurdle we have developed RiboSubstrates http://www.riboclub.org/ribosubstrates. This integrated bioinformatic software permits the searching of a cDNA database for all potential substrates for a given ribozyme. This includes the mRNAs that perfectly match the specific requirements of a given ribozyme, as well those including Wobble base pairs and mismatches. The results generated allow rapid selection of sequences suitable as targets for RNA degradation. The current web-based RiboSubstrates version permits the identification of potential gene targets for both SOFA-HDV ribozymes and for hammerhead ribozymes. Moreover, a minimal template for the search of siRNAs is also available. This flexible and reliable tool is easily adaptable for use with any RNA tool (i.e. other ribozymes, deoxyribozymes and antisense, and may use the information present in any cDNA bank. Conclusion RiboSubstrates should become an essential step for all, even including "non-RNA biologists", who endeavor to develop a gene-inactivation system.

  13. Inhibition of telomerase in tumor cells by ribozyme targeting telomerase RNA component

    Institute of Scientific and Technical Information of China (English)

    LIU; Bailin(刘柏林); QU; Yi(屈艺); LIU; Shuqiu(刘菽秋); OUYANG; Xuesong(欧阳雪松)

    2002-01-01

    Telomerase plays an important role in cell proliferation and carcinogenesis and is believed to be a good target for anti-cancer drugs. Elimination of template function of telomerase RNA may repress the telomerase activity. A hammer-headed ribozyme(telomerase ribozyme, teloRZ) directed against the RNA component of human telomerase(hTR) was designed and synthesized. TeloRZ showed a specific cleavage activity against the hTR. The cleavage efficacy reached 60%. A eukaryotic expression plasmid containing teloRZ gene was inducted into HeLa cells by lipofectamine, the telomerase activity in HeLa cells expressing teloRZ decreased to one eighth of that in the control cells. The doubling time increased significantly and the apoptosis ratio was elevated with increasing population doublings(PDS). After 19-20 PDS 95% cells were apoptotic. To further investigate the effect of teloRZ on tumor growth, the eukaryotic expression plasmid containing teloRZ was injected into transplanted tumor of nude mouse. The teloRZ effectively inhibited the telomerase activity in transplanted tumor, promoted apoptosis of the transplanted tumor cells, and decreased the tumor size significantly. These results indicate that teloRZ can effectively inhibit telomerase activity and growth of tumor cells, and suggest the potential use of this ribozyme in anti-cancer therapy.

  14. Viroid Replication: Rolling-Circles, Enzymes and Ribozymes

    Directory of Open Access Journals (Sweden)

    Marcos De la Peña

    2009-09-01

    Full Text Available Viroids, due to their small size and lack of protein-coding capacity, must rely essentially on their hosts for replication. Intriguingly, viroids have evolved the ability to replicate in two cellular organella, the nucleus (family Pospiviroidae and the chloroplast (family Avsunviroidae. Viroid replication proceeds through an RNA-based rolling-circle mechanism with three steps that, with some variations, operate in both polarity strands: i synthesis of longer-than-unit strands catalyzed by either the nuclear RNA polymerase II or a nuclear-encoded chloroplastic RNA polymerase, in both instances redirected to transcribe RNA templates, ii cleavage to unit-length, which in the family Avsunviroidae is mediated by hammerhead ribozymes embedded in both polarity strands, while in the family Pospiviroidae the oligomeric RNAs provide the proper conformation but not the catalytic activity, and iii circularization. The host RNA polymerases, most likely assisted by additional host proteins, start transcription from specific sites, thus implying the existence of viroid promoters. Cleavage and ligation in the family Pospiviroidae is probably catalyzed by an RNase III-like enzyme and an RNA ligase able to circularize the resulting 5’ and 3’ termini. Whether a chloroplastic RNA ligase mediates circularization in the family Avsunviroidae, or this reaction is autocatalytic, remains an open issue.

  15. Hidden ribozymes in eukaryotic genome sequence

    OpenAIRE

    Sean P Ryder

    2010-01-01

    The small self-cleaving ribozymes fold into complex tertiary structures to promote autocatalytic cleavage or ligation at a precise position within their sequence. Until recently, relatively few examples had been identified. Two papers now reveal that self-cleaving ribozymes are prevalent in eukaryotic genomes and, in some cases, might play a role in regulating gene expression.

  16. Molecular modelling of the GIR1 branching ribozyme gives new insight into evolution of structurally related ribozymes

    DEFF Research Database (Denmark)

    Beckert, Bertrand Dominique; Nielsen, Henrik; Einvik, Christer;

    2008-01-01

    Twin-ribozyme introns contain a branching ribozyme (GIR1) followed by a homing endonuclease (HE) encoding sequence embedded in a peripheral domain of a group I splicing ribozyme (GIR2). GIR1 catalyses the formation of a lariat with 3 nt in the loop, which caps the HE mRNA. GIR1 is structurally...

  17. Helium ion beam induced growth of hammerhead AFM probes

    NARCIS (Netherlands)

    Nanda, G.; Van Veldhoven, E.; Maas, D.; Sadeghian, H.; Alkemade, P.F.A.

    2015-01-01

    The authors report the direct-write growth of hammerhead atomic force microscope(AFM) probes by He+beam induced deposition of platinum-carbon. In order to grow a thin nanoneedle on top of a conventional AFM probe, the authors move a focused He+beam during exposure to a PtC precursor gas. In the fina

  18. Helium ion beam induced growth of hammerhead AFM probes

    NARCIS (Netherlands)

    Nanda, G.; Veldhoven, E. van; Maas, D.J.; Sadeghian Marnani, H.; Alkemade, P.F.A.

    2015-01-01

    The authors report the direct-write growth of hammerhead atomic force microscope (AFM) probes by He+ beam induced deposition of platinum-carbon. In order to grow a thin nanoneedle on top of a conventional AFM probe, the authors move a focused He+ beam during exposure to a PtC precursor gas. In the f

  19. Chemical fidelity of an RNA polymerase ribozyme

    DEFF Research Database (Denmark)

    Attwater, J.; Tagami, S.; Kimoto, M.;

    2013-01-01

    strands. Our results indicate specificity mechanisms that are found in functionally analogous forms in natural polymerases. They also reveal a level of chemical fidelity over multiple catalytic steps that is remarkable for a comparatively unoptimized enzyme developed de novo from a random sequence pool....... The convergent evolution of specificity mechanisms in phylogenetically unrelated proteinaceous polymerases and polymerase ribozymes suggests that chemical as well as informational fidelity are emergent properties of polymerase enzymes. © 2013 The Royal Society of Chemistry....

  20. MM 99.58 Physical modelling of Hammerhead forging, Vertical and Lateral load history

    DEFF Research Database (Denmark)

    Arentoft, Mogens; Eriksen, Morten; Wanheim, Tarras;

    1999-01-01

    The present report presents a laboratory setup with hammerhead forging, where the vertical and lateral force history is obtained under different process conditions......The present report presents a laboratory setup with hammerhead forging, where the vertical and lateral force history is obtained under different process conditions...

  1. Molecular characterization of a new member of the lariat capping twin-ribozyme introns

    DEFF Research Database (Denmark)

    Tang, Yunjia; Nielsen, Henrik; Masquida, Benoît;

    2014-01-01

    BACKGROUND: Twin-ribozyme introns represent a complex class of mobile group I introns that harbour a lariat capping (LC) ribozyme and a homing endonuclease gene embedded in a conventional self-splicing group I ribozyme (GIR2). Twin-ribozyme introns have so far been confined to nucleolar DNA in...... Naegleria amoeboflagellates and the myxomycete Didymium iridis. RESULTS: We characterize structural organization, catalytic properties and molecular evolution of a new twin-ribozyme intron in Allovahlkampfia (Heterolobosea). The intron contains two ribozyme domains with different functions in ribosomal RNA...... efficient self-cleaving ribozyme that generates a small 2',5' lariat cap at the 5' end of the homing endonuclease mRNA, and thus contributes to intron mobility. CONCLUSIONS: The discovery of a twin-ribozyme intron in a member of Heterolobosea expands the distribution pattern of LC ribozymes. We identify a...

  2. Chemistry and Biology of Self-Cleaving Ribozymes.

    Science.gov (United States)

    Jimenez, Randi M; Polanco, Julio A; Lupták, Andrej

    2015-11-01

    Self-cleaving ribozymes were discovered 30 years ago, but their biological distribution and catalytic mechanisms are only beginning to be defined. Each ribozyme family is defined by a distinct structure, with unique active sites accelerating the same transesterification reaction across the families. Biochemical studies show that general acid-base catalysis is the most common mechanism of self-cleavage, but metal ions and metabolites can be used as cofactors. Ribozymes have been discovered in highly diverse genomic contexts throughout nature, from viroids to vertebrates. Their biological roles include self-scission during rolling-circle replication of RNA genomes, co-transcriptional processing of retrotransposons, and metabolite-dependent gene expression regulation in bacteria. Other examples, including highly conserved mammalian ribozymes, suggest that many new biological roles are yet to be discovered. PMID:26481500

  3. Kinetic framework for ligation by an efficient RNA ligase ribozyme.

    Science.gov (United States)

    Bergman, N H; Johnston, W K; Bartel, D P

    2000-03-21

    The class I RNA ligase ribozyme, isolated previously from random sequences, performs an efficient RNA ligation reaction. It ligates two substrate RNAs, promoting the attack of the 3'-hydroxyl of one substrate upon the 5'-triphosphate of the other substrate with release of pyrophosphate. This ligation reaction has similarities to the reaction catalyzed by RNA polymerases. Using data from steady-state kinetic measurements and pulse-chase/pH-jump experiments, we have constructed minimal kinetic frameworks for two versions of the class I ligase, named 207t and 210t. For both ligases, as well as for the self-ligating parent ribozyme, the rate constant for the chemical step (k(c)) is log-linear with pH in the range 5.7-8.0. At physiological pH, the k(c) is 100 min(-1), a value similar to those reported for the fastest naturally occurring ribozymes. At higher pH, product release is limiting for both 207t and 210t. The 210t ribozyme, with its faster product release, attains multiple-turnover rates (k(cat) = 360 min(-1), pH 9.0) exceeding those of 207t and other reported ribozyme reactions. The kinetic framework for the 210t ribozyme describes the limits of this catalysis and suggests how key steps can be targeted for improvement using design or combinatorial approaches. PMID:10715133

  4. Electroreception in juvenile scalloped hammerhead and sandbar sharks.

    Science.gov (United States)

    Kajiura, Stephen M; Holland, Kim N

    2002-12-01

    The unique head morphology of sphyrnid sharks might have evolved to enhance electrosensory capabilities. The 'enhanced electroreception' hypothesis was tested by comparing the behavioral responses of similarly sized carcharhinid and sphyrnid sharks to prey-simulating electric stimuli. Juvenile scalloped hammerhead sharks Sphyrna lewini and sandbar sharks Carcharhinus plumbeus oriented to dipole electric fields from the same maximum distance (approximately 30 cm) and thus demonstrated comparable behavioral-response thresholds (shark bent its trunk to orient to the center of the dipole. By contrast, sandbars swam in a broader arc towards the center of the dipole. The different orientation patterns are attributed to the hydrodynamic properties of the cephalofoil, which enables the hammerheads to execute sharp turns at high speed. The greater trunk width of the sandbar sharks prevented them from demonstrating the same degree of flexibility. Therefore, although the sphyrnid head morphology does not appear to confer a greater sensitivity to prey-simulating dipole electric fields, it does provide (1). a greater lateral search area, which may increase the probability of prey encounter, and (2). enhanced maneuverability, which may aid in prey capture. PMID:12409487

  5. Modulation of Group I Ribozyme Activity by Cationic Porphyrins

    Directory of Open Access Journals (Sweden)

    Shigeyoshi Matsumura

    2015-03-01

    Full Text Available The effects of cationic porphyrins on the catalytic activities of four group I ribozymes were investigated. A cationic porphyrin possessing four pyridinium moieties (pPyP inhibited two group IC3 ribozymes (Syn Rz and Azo Rz and a group IC1 ribozyme (Tet Rz. In the case of a group IA2 ribozyme (Td Rz, however, pPyP served not only as an inhibitor but also as an activator, and the effects of pPyP were dependent on its concentration. To analyze the structural and electronic factors determining the effects of pPyP on group I ribozymes, three cationic porphyrins (pPyNCP, pPyF4P, and TMPyP were also examined. As interactions between small organic molecules and nucleic acids are attractive and important issues in biochemistry and biotechnology, this study contributes to the development of porphyrin-based molecules that can modulate functions of structured RNA molecules.

  6. Constructional morphology within the head of hammerhead sharks (sphyrnidae).

    Science.gov (United States)

    Mara, Kyle R; Motta, Philip J; Martin, Andrew P; Hueter, Robert E

    2015-05-01

    The study of functional trade-offs is important if a structure, such as the cranium, serves multiple biological roles, and is, therefore, shaped by multiple selective pressures. The sphyrnid cephalofoil presents an excellent model for investigating potential trade-offs among sensory, neural, and feeding structures. In this study, hammerhead shark species were chosen to represent differences in head form through phylogeny. A combination of surface-based geometric morphometrics, computed tomography (CT) volumetric analysis, and phylogenetic analyses were utilized to investigate potential trade-offs within the head. Hammerhead sharks display a diversity of cranial morphologies where the position of the eyes and nares vary among species, with only minor changes in shape, position, and volume of the feeding apparatus through phylogeny. The basal winghead shark, Eusphyra blochii, has small anteriorly positioned eyes. Through phylogeny, the relative size and position of the eyes change, such that derived species have larger, more medially positioned eyes. The lateral position of the external nares is highly variable, showing no phylogenetic trend. Mouth size and position are conserved, remaining relatively unchanged. Volumetric CT analyses reveal no trade-offs between the feeding apparatus and the remaining cranial structures. The few trade-offs were isolated to the nasal capsule volume's inverse correlation with braincase, chondrocranial, and total cephalofoil volume. Eye volume also decreased as cephalofoil width increased. These data indicate that despite considerable changes in head shape, much of the head is morphologically conserved through sphyrnid phylogeny, particularly the jaw cartilages and their associated feeding muscles, with shape change and morphological trade-offs being primarily confined to the lateral wings of the cephalofoil and their associated sensory structures. PMID:25684106

  7. Speciation of a group I intron into a lariat capping ribozyme

    DEFF Research Database (Denmark)

    Meyer, Mélanie; Nielsen, Henrik; Oliéric, Vincent;

    2014-01-01

    The lariat-capping (LC) ribozyme is a natural ribozyme isolated from eukaryotic microorganisms. Despite apparent structural similarity to group I introns, the LC ribozyme catalyzes cleavage by a 2',5' branching reaction, leaving the 3' product with a 3-nt lariat cap that functionally substitutes...... for a conventional mRNA cap in the downstream pre-mRNA encoding a homing endonuclease. We describe the crystal structures of the precleavage and postcleavage LC ribozymes, which suggest that structural features inherited from group I ribozymes have undergone speciation due to profound changes in...

  8. Cleavage of highly structured viral RNA molecules by combinatorial libraries of hairpin ribozymes. The most effective ribozymes are not predicted by substrate selection rules.

    Science.gov (United States)

    Yu, Q; Pecchia, D B; Kingsley, S L; Heckman, J E; Burke, J M

    1998-09-01

    Combinatorial libraries of hairpin ribozymes representing all possible cleavage specificities (>10(5)) were used to evaluate all ribozyme cleavage sites within a large (4.2-kilobase) and highly structured viral mRNA, the 26 S subgenomic RNA of Sindbis virus. The combinatorial approach simultaneously accounts for target site structure and dynamics, together with ribozyme folding, and the sequences that result in a ribozyme-substrate complex with maximal activity. Primer extension was used to map and rank the relative activities of the ribozyme pool against individual sites and revealed two striking findings. First, only a small fraction of potential recognition sites are effectively cleaved (activity-selected sites). Second, nearly all of the most effectively cleaved sites deviated substantially from the established consensus selection rules for the hairpin ribozyme and were not predicted by examining the sequence, or through the use of computer-assisted predictions of RNA secondary structure. In vitro selection methods were used to isolate ribozymes with increased activity against substrates that deviate from the GUC consensus sequence. trans-Acting ribozymes targeting nine of the activity-selected sites were synthesized, together with ribozymes targeting four sites with a perfect match to the cleavage site consensus (sequence-selected sites). Activity-selected ribozymes have much higher cleavage activity against the long, structured RNA molecules than do sequence-selected ribozymes, although the latter are effective in cleaving oligoribonucleotides, as predicted. These results imply that, for Sindbis virus 26 S RNA, designing ribozymes based on matches to the consensus sequence may be an ineffective strategy. PMID:9722591

  9. Fluorescent RNA labeling using self-alkylating ribozymes.

    Science.gov (United States)

    Sharma, Ashwani K; Plant, Joshua J; Rangel, Alexandra E; Meek, Kirsten N; Anamisis, April J; Hollien, Julie; Heemstra, Jennifer M

    2014-08-15

    The ability to fluorescently label specific RNA sequences is of significant utility for both in vitro and live cell applications. Currently, most RNA labeling methods utilize RNA-nucleic acid or RNA-protein molecular recognition. However, in the search for improved RNA labeling methods, harnessing the small-molecule recognition capabilities of RNA is rapidly emerging as a promising alternative. Along these lines, we propose a novel strategy in which a ribozyme acts to promote self-alkylation with a fluorophore, providing a robust, covalent linkage between the RNA and the fluorophore. Here we describe the selection and characterization of ribozymes that promote self-labeling with fluorescein iodoacetamide (FIA). Kinetic studies reveal a second-order rate constant that is on par with those of other reactions used for biomolecular labeling. Additionally, we demonstrate that labeling is specific to the ribozyme sequences, as FIA does not react nonspecifically with RNA. PMID:24896502

  10. The Structural Basis of Ribozyme-Catalyzed RNA Assembly

    Energy Technology Data Exchange (ETDEWEB)

    Robertson, M.P.; Scott, W.G.; /UC, Santa Cruz

    2007-07-12

    Life originated, according to the RNA World hypothesis, from self-replicating ribozymes that catalyzed ligation of RNA fragments. We have solved the 2.6 angstrom crystal structure of a ligase ribozyme that catalyzes regiospecific formation of a 5' to 3' phosphodiester bond between the 5'-triphosphate and the 3'-hydroxyl termini of two RNA fragments. Invariant residues form tertiary contacts that stabilize a flexible stem of the ribozyme at the ligation site, where an essential magnesium ion coordinates three phosphates. The structure of the active site permits us to suggest how transition-state stabilization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly.

  11. Structural studies on an internal loop from a hairpin ribozyme

    Energy Technology Data Exchange (ETDEWEB)

    Cai, Z.; SantaLucia, J. Jr.; Tinoco, I. Jr. [Univ. of California, Berkeley, CA (United States)

    1994-12-01

    Ribozymes, RNA enzymes, catalyze site-specific RNA cleavage and ligation reactions. We are studying the three-dimensional structure of a hairpin ribozyme derived from the minus strand of tobacco ring spot virus satellite RNA ((-)sTRSV), which has been engineering to specifically cleave the HIV-1 RNA. The minimum structure for the catalytic reaction involves a 50-nucleotide ribozyme and a 14-nucleotide substrate. The proposed secondary structure of the ribozyme-substrate complex consists of four short helices separated by two internal loops. The relatively large size (64-nucleotide) of the ribozyme-substrate complex presents formidable problems in solving the structure using NMR. Therefore we are studying smaller structural subunits of the complex. We are determining the high resolution structure of the symmetric internal loop involving the cleavage site and the flanking helices. One strand of the internal loop was selectively {sup 13}C-labeled at C8 of each purine and C6 of each pyrimidine. By using {sup 13}C-edited two-dimensional NMR, the proton NOESY spectrum was greatly simplified. This allowed unambiguous sequential proton resonance assignments along each strand. Three-dimensional {sup 1}-{sup 13}C HMQC-NOESY was used to further facilitate resonance assignments. We are also enzymatically synthesizing the entire 50-nucleotide ribozyme and will combine it with the {sup 13}C-labeled substrate. Through comparison of the NOE connectivities of the labeled nucleotides from the internal loop alone with those from the entire complex, the differences between the two structures can be elucidated.

  12. Kinetic partitioning mechanism of HDV ribozyme folding

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Jiawen; Gong, Sha; Wang, Yujie; Zhang, Wenbing, E-mail: wbzhang@whu.edu.cn [Department of Physics, Wuhan University, Wuhan, Hubei 430072 (China)

    2014-01-14

    RNA folding kinetics is directly tied to RNA biological functions. We introduce here a new approach for predicting the folding kinetics of RNA secondary structure with pseudoknots. This approach is based on our previous established helix-based method for predicting the folding kinetics of RNA secondary structure. In this approach, the transition rates for an elementary step: (1) formation, (2) disruption of a helix stem, and (3) helix formation with concomitant partial melting of an incompatible helix, are calculated with the free energy landscape. The folding kinetics of the Hepatitis delta virus (HDV) ribozyme and the mutated sequences are studied with this method. The folding pathways are identified by recursive searching the states with high net flux-in(out) population starting from the native state. The theory results are in good agreement with that of the experiments. The results indicate that the bi-phasic folding kinetics for the wt HDV sequence is ascribed to the kinetic partitioning mechanism: Part of the population will quickly fold to the native state along the fast pathway, while another part of the population will fold along the slow pathway, in which the population is trapped in a non-native state. Single mutation not only changes the folding rate but also the folding pathway.

  13. Reconciling Ligase Ribozyme Activity with Fatty Acid Vesicle Stability

    OpenAIRE

    Fabrizio Anella; Christophe Danelon

    2014-01-01

    The “RNA world” and the “Lipid world” theories for the origin of cellular life are often considered incompatible due to the differences in the environmental conditions at which they can emerge. One obstacle resides in the conflicting requirements for divalent metal ions, in particular Mg2+, with respect to optimal ribozyme activity, fatty acid vesicle stability and protection against RNA strand cleavage. Here, we report on the activity of a short L1 ligase ribozyme in the presence of myristol...

  14. 78 FR 24701 - Endangered and Threatened Wildlife; 90-Day Finding on Petitions To List the Great Hammerhead...

    Science.gov (United States)

    2013-04-26

    ... hammerhead shark is considered to be a candidate species (69 FR 19975; April 15, 2004). Within 12 months of..., to list the great hammerhead shark range-wide as threatened, and to designate critical habitat. The...'' for the purposes of listing, delisting, and reclassifying a species under the ESA (61 FR...

  15. Kinematics and critical swimming speed of juvenile scalloped hammerhead sharks

    Science.gov (United States)

    Lowe

    1996-01-01

    Kinematics and critical swimming speed (Ucrit) of juvenile scalloped hammerhead sharks Sphyrna lewini were measured in a Brett-type flume (635 l). Kinematic parameters were also measured in sharks swimming in a large pond for comparison with those of sharks swimming in the flume. Sharks in the flume exhibited a mean Ucrit of 65±11 cm s-1 (± s.d.) or 1.17±0.21 body lengths per second (L s-1), which are similar to values for other species of sharks. In both the flume and pond, tailbeat frequency (TBF) and stride length (LS) increased linearly with increases in relative swimming speed (Urel=body lengths traveled per second). In the flume, tailbeat amplitude (TBA) decreased with increasing speed whereas TBA did not change with speed in the pond. Differences in TBF and LS between sharks swimming in the flume and the pond decreased with increases in Urel. Sharks swimming at slow speeds (e.g. 0.55 L s-1) in the pond had LS 19 % longer and TBF 21 % lower than sharks in the flume at the same Urel. This implies that sharks in the flume expended more energy while swimming at comparable velocities. Comparative measurements of swimming kinematics from sharks in the pond can be used to correct for effects of the flume on shark swimming kinematics and energetics. PMID:9320537

  16. A Hairpin Ribozyme Inhibits Expression of Diverse Strains of Human Immunodeficiency Virus Type 1

    Science.gov (United States)

    Yu, Mang; Ojwang, Joshua; Yamada, Osamu; Hampel, Arnold; Rapapport, Jay; Looney, David; Wong-Staal, Flossie

    1993-07-01

    Ribozymes have enormous potential as antiviral agents. We have previously reported that a hairpin ribozyme expressed under the control of the β-actin promoter that cleaves human immunodeficiency virus type 1 (HIV-1) RNA in the leader sequence can inhibit HIV-1 (pHXB2gpt) expression. For such a ribozyme in a retroviral vector delivery system to be useful in gene therapy for the treatment of HIV-1 infection, it must be able to inhibit the expression of multiple HIV-1 strains. We have now cloned this ribozyme into various regular expression vectors (including retroviral vectors) by using various gene expression control strategies. Here we show by transient transfection that inhibition of expression of diverse strains of HIV-1 can be achieved by this ribozyme expressed in the proper vectors. These data further support the potential of this hairpin ribozyme as a therapeutic agent for HIV-1.

  17. An mRNA is capped by a 2', 5' lariat catalyzed by a group I-like ribozyme

    DEFF Research Database (Denmark)

    Nielsen, Henrik; Westhof, Eric; Johansen, Steinar

    2005-01-01

    Twin-ribozyme introns are formed by two ribozymes belonging to the group I family and occur in some ribosomal RNA transcripts. The group I-like ribozyme, GIR1, liberates the 5' end of a homing endonuclease messenger RNA in the slime mold Didymium iridis. We demonstrate that this cleavage occurs...

  18. Reconciling Ligase Ribozyme Activity with Fatty Acid Vesicle Stability

    Directory of Open Access Journals (Sweden)

    Fabrizio Anella

    2014-12-01

    Full Text Available The “RNA world” and the “Lipid world” theories for the origin of cellular life are often considered incompatible due to the differences in the environmental conditions at which they can emerge. One obstacle resides in the conflicting requirements for divalent metal ions, in particular Mg2+, with respect to optimal ribozyme activity, fatty acid vesicle stability and protection against RNA strand cleavage. Here, we report on the activity of a short L1 ligase ribozyme in the presence of myristoleic acid (MA vesicles at varying concentrations of Mg2+. The ligation rate is significantly lower at low-Mg2+ conditions. However, the loss of activity is overcompensated by the increased stability of RNA leading to a larger amount of intact ligated substrate after long reaction periods. Combining RNA ligation assays with fatty acid vesicles we found that MA vesicles made of 5 mM amphiphile are stable and do not impair ligase ribozyme activity in the presence of approximately 2 mM Mg2+. These results provide a scenario in which catalytic RNA and primordial membrane assembly can coexist in the same environment.

  19. Social organization of schools of the Scalloped Hammerhead shark, Sphyrna lewini (Griffith and Smith), in the Gulf of California

    OpenAIRE

    Klimley, A. Peter

    1983-01-01

    The social behavior of the scalloped hammerhead shark is described with an emphasis on determining the function of its polarized schooling. . This shark possesses a refuging social system in which individuals remain in groups in a small core of their home range during the inactive phase of their diel cycle and disperse into the surrounding environment during the active phase. Scalloped hammerhead sharks, followed by ultrasonic telemetry, swam slowly back and forth during the day along the rid...

  20. Generation and Development of RNA Ligase Ribozymes with Modular Architecture Through “Design and Selection”

    Directory of Open Access Journals (Sweden)

    Yuki Fujita

    2010-08-01

    Full Text Available In vitro selection with long random RNA libraries has been used as a powerful method to generate novel functional RNAs, although it often requires laborious structural analysis of isolated RNA molecules. Rational RNA design is an attractive alternative to avoid this laborious step, but rational design of catalytic modules is still a challenging task. A hybrid strategy of in vitro selection and rational design has been proposed. With this strategy termed “design and selection,” new ribozymes can be generated through installation of catalytic modules onto RNA scaffolds with defined 3D structures. This approach, the concept of which was inspired by the modular architecture of naturally occurring ribozymes, allows prediction of the overall architectures of the resulting ribozymes, and the structural modularity of the resulting ribozymes allows modification of their structures and functions. In this review, we summarize the design, generation, properties, and engineering of four classes of ligase ribozyme generated by design and selection.

  1. Intracellular ribozyme-catalyzed trans-cleavage of RNA monitored by fluorescence resonance energy transfer.

    Science.gov (United States)

    Vitiello, D; Pecchia, D B; Burke, J M

    2000-04-01

    Small catalytic RNAs like the hairpin ribozyme are proving to be useful intracellular tools; however, most attempts to demonstrate trans-cleavage of RNA by ribozymes in cells have been frustrated by rapid cellular degradation of the cleavage products. Here, we describe a fluorescence resonance energy transfer (FRET) assay that directly monitors cleavage of target RNA in tissue-culture cells. An oligoribonucleotide substrate was modified to inhibit cellular ribonuclease degradation without interfering with ribozyme cleavage, and donor (fluorescein) and acceptor (tetramethylrhodamine) fluorophores were introduced at positions flanking the cleavage site. In simple buffers, the intact substrate produces a strong FRET signal that is lost upon cleavage, resulting in a red-to-green shift in dominant fluorescence emission. Hairpin ribozyme and fluorescent substrate were microinjected into murine fibroblasts under conditions in which substrate cleavage can occur only inside the cell. A strong FRET signal was observed by fluorescence microscopy when substrate was injected, but rapid decay of the FRET signal occurred when an active, cognate ribozyme was introduced with the substrate. No acceleration in cleavage rates was observed in control experiments utilizing a noncleavable substrate, inactive ribozyme, or an active ribozyme with altered substrate specificity. Subsequently, the fluorescent substrates were injected into clonal cell lines that expressed cognate or noncognate ribozymes. A decrease in FRET signal was observed only when substrate was microinjected into cells expressing its cognate ribozyme. These results demonstrate trans-cleavage of RNA within mammalian cells, and provide an experimental basis for quantitative analysis of ribozyme activity and specificity within the cell. PMID:10786853

  2. In vitro evolution of coenzyme-independent variants from the glmS ribozyme structural scaffold.

    Science.gov (United States)

    Lau, Matthew W L; Ferré-D'Amaré, Adrian R

    2016-08-15

    Uniquely among known natural ribozymes that cleave RNA sequence-specifically, the glmS ribozyme-riboswitch employs a small molecule, glucosamine-6-phosphate (GlcN6P) as a catalytic cofactor. In vitro selection was employed to search for coenzyme-independent variants of this ribozyme. In addition to shedding light on the catalytic mechanism of the ribozyme, such variants could resemble the evolutionary ancestors of the modern, GlcN6P-regulated ribozyme-riboswitch. A mutant pool was constructed such that the secondary structure elements, which define the triply-pseudoknotted global fold of the ribozyme, was preserved. A stringent selection scheme that relies on thiol-mercury affinity chromatography for separating active and inactive sequences ultimately yielded a triple mutant with a cleavage rate exceeding 3min(-1) that only requires divalent cations for activity. Mutational analysis demonstrated that a point reversion of the variant toward the wild-type sequence was sufficient to partially restore GlcN6P-dependence, suggesting that coenzyme dependence can be readily be acquired by RNAs that adopt the glmS ribozyme fold. The methods employed to perform this selection experiment are described in detail in this review. PMID:27130889

  3. Age validation of great hammerhead shark (Sphyrna mokarran), determined by bomb radiocarbon analysis

    OpenAIRE

    Passerotti, Michelle S.; John K. Carlson; Piercy, Andrew N.; Campana, Steven E.

    2010-01-01

    Preliminary validation of annual growth band deposition in vertebrae of great hammerhead shark (Sphyrna mokarran) was conducted by using bomb radiocarbon analysis. Adult specimens (n=2) were collected and thin sections of vertebral centra were removed for visual aging and use in radiocarbon assays. Vertebral band counts were used to estimate age, and year of formation was assigned to each growth band by subtracting estimated age from the year of capture. A total of 10 samples were extra...

  4. Effects of fasting and refeeding on intestinal cell proliferation and apoptosis in hammerhead shark (Sphyrna lewini)

    OpenAIRE

    Hideya Takahashi; Susumu Hyodo; Tsukasa Abe; Chiyo Takagi; Grau, Gordon E.; Tatsuya Sakamoto

    2014-01-01

    Objective: To examine the effects of fasting and refeeding on intestinal cell proliferation and apoptosis in an opportunistic predator, hammerhead shark (Sphyrna lewini) of elasmobranch fishes which are among the earliest known extant groups of vertebrates to have the valvular intestine typical for the primitive species. Methods: Animals were euthanized after 5-10 d of fasting or feeding, or after 10-day fasting and 5-day refeeding. Intestinal apoptosis and cell proliferation w...

  5. Isolation of novel ribozymes that ligate AMP-activated RNA substrates

    Science.gov (United States)

    Hager, A. J.; Szostak, J. W.

    1997-01-01

    BACKGROUND: The protein enzymes RNA ligase and DNA ligase catalyze the ligation of nucleic acids via an adenosine-5'-5'-pyrophosphate 'capped' RNA or DNA intermediate. The activation of nucleic acid substrates by adenosine 5'-monophosphate (AMP) may be a vestige of 'RNA world' catalysis. AMP-activated ligation seems ideally suited for catalysis by ribozymes (RNA enzymes), because an RNA motif capable of tightly and specifically binding AMP has previously been isolated. RESULTS: We used in vitro selection and directed evolution to explore the ability of ribozymes to catalyze the template-directed ligation of AMP-activated RNAs. We subjected a pool of 10(15) RNA molecules, each consisting of long random sequences flanking a mutagenized adenosine triphosphate (ATP) aptamer, to ten rounds of in vitro selection, including three rounds involving mutagenic polymerase chain reaction. Selection was for the ligation of an oligonucleotide to the 5'-capped active pool RNA species. Many different ligase ribozymes were isolated; these ribozymes had rates of reaction up to 0.4 ligations per hour, corresponding to rate accelerations of approximately 5 x10(5) over the templated, but otherwise uncatalyzed, background reaction rate. Three characterized ribozymes catalyzed the formation of 3'-5'-phosphodiester bonds and were highly specific for activation by AMP at the ligation site. CONCLUSIONS: The existence of a new class of ligase ribozymes is consistent with the hypothesis that the unusual mechanism of the biological ligases resulted from a conservation of mechanism during an evolutionary replacement of a primordial ribozyme ligase by a more modern protein enzyme. The newly isolated ligase ribozymes may also provide a starting point for the isolation of ribozymes that catalyze the polymerization of AMP-activated oligonucleotides or mononucleotides, which might have been the prebiotic analogs of nucleoside triphosphates.

  6. An improved version of the hairpin ribozyme functions as a ribonucleoprotein complex.

    Science.gov (United States)

    Sargueil, B; Pecchia, D B; Burke, J M

    1995-06-13

    Most RNA molecules that are endowed with catalytic activity function in the form of ribonucleoproteins within cells. These complexes are frequently large, poorly defined, and difficult to study. As a model system to study biological catalysis by ribonucleoproteins, we have modified the hairpin ribozyme by inserting an RNA structure that serves as a binding site for bacteriophage R17 coat protein in the form of an extension to ribozyme helix 4, which lies at the periphery of the catalytic domain. In the absence of protein, we find that incorporation of the protein-binding domain increases the catalytic efficiency of the hairpin ribozyme by 2-fold for the cleavage reaction and 16-fold for the ligation reaction. This increase in activity correlates with an increase in the proportion of molecules which fold into the active tertiary structure, as measured by a UV cross-linking assay. Mobility-shift and filter-binding assays of complex formation show that R17 coat protein binds to the chimeric ribozyme with a dissociation constant essentially identical to that of the isolated protein-binding domain; no binding of the protein to the unmodified ribozyme could be detected. The kinetics of cleavage and ligation reactions are not altered by the presence of saturating concentrations of coat protein, and competition studies demonstrate that the protein remains bound to the ribozyme throughout the catalytic cycle. These studies establish that the hairpin ribozyme can be engineered to function efficiently in the form of a ribonucleoprotein in vitro and will serve as the basis for future experimentation to understand mechanisms of protein modulation of catalytic RNA activity, and to introduce other protein-binding domains, for example, HIV-1 rev-binding and tar elements, which may be useful for influencing subcellular localization, regulating intracellular activity, or generating ribozymes that also function as "decoys" in antiviral applications. PMID:7779820

  7. Intracellular ribozyme-catalyzed trans-cleavage of RNA monitored by fluorescence resonance energy transfer.

    OpenAIRE

    Vitiello, D; Pecchia, D B; Burke, J M

    2000-01-01

    Small catalytic RNAs like the hairpin ribozyme are proving to be useful intracellular tools; however, most attempts to demonstrate trans-cleavage of RNA by ribozymes in cells have been frustrated by rapid cellular degradation of the cleavage products. Here, we describe a fluorescence resonance energy transfer (FRET) assay that directly monitors cleavage of target RNA in tissue-culture cells. An oligoribonucleotide substrate was modified to inhibit cellular ribonuclease degradation without int...

  8. Putative intermediary stages for the molecular evolution from a ribozyme to a catalytic RNP

    OpenAIRE

    IKAWA, YOSHIYA; Tsuda, Kentaro; Matsumura, Shigeyoshi; Atsumi, Shota; Inoue, Tan

    2003-01-01

    A hypothetical evolutionary pathway from a ribozyme to a catalytic RNA–protein complex (RNP) is proposed and examined. In this hypothesis for an early phase of molecular evolution, one RNA–RNA interaction in the starting ribozyme is replaced with an RNA–protein interaction via two intermediary stages. At each stage, the original RNA–RNA interaction and a newly introduced RNA–protein interaction are designed to coexist. The catalytic RNPs corresponding to the intermediary stages were construct...

  9. A comprehensive study of ribozyme-mediated nucleotide and sugar chemistries

    OpenAIRE

    Lau, Matthew

    2009-01-01

    The ability of RNA catalysts (ribozymes) to synthesize nucleotides as basic building blocks for the assembly of larger RNA polymers is an important aspect of the 'RNA World' hypothesis. To examine the ability of RNAs to catalyze this chemistry, we performed an in vitro selection in my first project and successfully isolated ribozymes capable of synthesizing a purine nucleotide (6-thioguanosine, 6SG) from tethered 5-phosphoribosyl 1-pyrophosphate (PRPP) and 6-thioguanine (6SGua). Compared to t...

  10. Role of SLV in SLI substrate recognition by the Neurospora VS ribozyme

    OpenAIRE

    Bouchard, Patricia; Lacroix-Labonté, Julie; Desjardins, Geneviève; Lampron, Philipe; Lisi, Véronique; Lemieux, Sébastien; Major, François; Legault, Pascale

    2008-01-01

    Substrate recognition by the VS ribozyme involves a magnesium-dependent loop/loop interaction between the SLI substrate and the SLV hairpin from the catalytic domain. Recent NMR studies of SLV demonstrated that magnesium ions stabilize a U-turn loop structure and trigger a conformational change for the extruded loop residue U700, suggesting a role for U700 in SLI recognition. Here, we kinetically characterized VS ribozyme mutants to evaluate the contribution of U700 and other SLV loop residue...

  11. Cleavage of an amide bond by a ribozyme

    Science.gov (United States)

    Dai, X.; De Mesmaeker, A.; Joyce, G. F.; Miller, S. L. (Principal Investigator)

    1995-01-01

    A variant form of a group I ribozyme, optimized by in vitro evolution for its ability to catalyze magnesium-dependent phosphoester transfer reactions involving DNA substrates, also catalyzes the cleavage of an unactivated alkyl amide when that linkage is presented in the context of an oligodeoxynucleotide analog. Substrates containing an amide bond that joins either two DNA oligos, or a DNA oligo and a short peptide, are cleaved in a magnesium-dependent fashion to generate the expected products. The first-order rate constant, kcat, is 0.1 x 10(-5) min-1 to 1 x 10(-5) min-1 for the DNA-flanked substrates, which corresponds to a rate acceleration of more than 10(3) as compared with the uncatalyzed reaction.

  12. Maternal offloading of organochlorine contaminants in the yolk-sac placental scalloped hammerhead shark (Sphyrna lewini).

    Science.gov (United States)

    Lyons, Kady; Adams, Douglas H

    2015-04-01

    Elasmobranchs are a group of animals that typically occupy upper trophic levels in food webs and have a propensity to accumulate high contaminant concentrations. To date, few studies have investigated maternal offloading processes in sharks, despite the fact that this process represents a substantial source of exposure for young sharks and is a significant pathway for contaminant redistribution within marine ecosystems. Comparable to mammalian systems, scalloped hammerhead sharks (Sphyrna lewini) utilize a yolk-sac placental strategy to nourish young in utero, which may allow females to transfer contaminants to young. Organic contaminants (PCBs and chlorinated pesticides) were measured in livers of both females and males from several age classes that were collected from U.S. Atlantic waters, including two near-term pregnant females and their embryos. Adult female hammerheads (n = 3) were found to have lower levels of PCBs compared to the younger, adult male (mean ± SD, 11.1 ± 1.0 vs. 22.8 μg g(-1) lw), but had substantially higher concentrations of pesticides (4.1 ± 0.9 vs. 1.9 μg g(-1) lw). Embryos from the two litters (n = 36) had similar levels of summed organic contaminant concentrations (4.6 ± 0.9 μg g(-1) lw) and pregnant females were estimated to offload approximately 0.03-2.3% of their hepatic contaminant load to offspring. While the potential health impacts of these transferred contaminants is unknown, this is the first study to demonstrate that scalloped hammerheads are exposed to a substantial amount of contaminants prior to birth and document maternal offloading of organochlorines in a pseudo-placental shark species. Therefore, future research should continue to investigate the potential adverse effects these contaminants have on elasmobranch physiology. PMID:25527298

  13. Vulnerability to longline fisheries of three hammerhead shark Sphyrna species in the south-western and equatorial Atlantic Ocean.

    Science.gov (United States)

    Bezerra, N P A; Travassos, P; Hazin, F H V

    2016-08-01

    Catch and effort data from 29 418 longline sets from Brazilian tuna longline vessels operating in the south-western and equatorial Atlantic Ocean between 2004 and 2011 were analysed to investigate the distribution, catch rate and size of three species of hammerhead sharks (Sphyrna lewini, Sphyrna mokarran and Sphyrna zygaena). During that period, 6172 hammerhead sharks were caught. Among the elasmobranchs, the highest percentage of hammerhead sharks were caught in 2007, when they accounted for 3·90% of the group, while the lowest value of 0·40% was recorded in 2010. In general, the spatial distribution of the mean catch per unit effort (CPUE) by years and quarters showed a trend of higher catches near the equatorial region and in southern Brazil. The nominal mean CPUE was 0·12 Sphyrna spp. 1000(-1) hooks, with the highest value being recorded in 2007 (0·30 Sphyrna spp. 1000(-1) hooks). The standardized yearly CPUE estimated by a generalized linear model assuming a zero inflated negative binomial (ZINB) distribution were not much different from nominal values. Of the 205 sexed specimens, 117 were females and 88 were males, resulting in a sex ratio with a predominance of females (1·30:1·00), although not statistically significant. The total length of females ranged from 1200 to 2800 mm and of males from 1100 to 3100 mm. Juvenile hammerhead sharks represented 82 and 54% of the sexed female and male specimens, respectively. PMID:27349350

  14. Conservation Genetics of the Scalloped Hammerhead Shark in the Pacific Coast of Colombia.

    Science.gov (United States)

    Quintanilla, Sonia; Gómez, Alberto; Mariño-Ramírez, Camila; Sorzano, Carolina; Bessudo, Sandra; Soler, German; Bernal, Jaime E; Caballero, Susana

    2015-01-01

    Previous investigations of the population genetics of the scalloped hammerhead sharks (Sphyrna lewini) in the Eastern Tropical Pacific have lacked information about nursery areas. Such areas are key to promoting conservation initiatives that can protect young sharks from threats such as overfishing. Here, we investigated the genetic diversity, phylogeography, and connectivity of S. lewini found in 3 areas of Colombia's Pacific coast: around Malpelo Island and in 2 National Natural Parks on the Colombian Pacific mainland (Sanquianga and Ensenada de Utría). We analyzed mtDNA control region (CR) sequences and genotyped 15 microsatellite loci in 137 samples of adults and juveniles. The mtDNA analyses showed haplotypes shared between the Colombian Pacific individuals sampled in this investigation and other areas in the Eastern Tropical Pacific, the Indo-Pacific, and with sequences previously reported in Colombia (Buenaventura Port), as well as 4 unique haplotypes. Population assignment and paternity analyses detected 3 parent-offspring pairs between Malpelo and Sanquianga and 1 between Malpelo and Utría. These results indicate high genetic connectivity between Malpelo Island and the Colombian Pacific coast, suggesting that these 2 areas are nurseries for S. lewini. This is, to our knowledge, the first evidence of nursery areas identified for the scalloped hammerhead shark anywhere in the world. Additional conservation planning may be required to protect these nursery habitats of this endangered shark species. PMID:26245780

  15. The complement system of elasmobranches revealed by liver transcriptome analysis of a hammerhead shark, Sphyrna zygaena.

    Science.gov (United States)

    Goshima, Masayuki; Sekiguchi, Reo; Matsushita, Misao; Nonaka, Masaru

    2016-08-01

    Comprehensive studies of the complement genes in basal vertebrates have revealed that cyclostomes have apparently primitive complement systems whereas bony fish have well-developed complement systems comparable to those of mammals. Here we have performed liver transcriptome analysis of a hammerhead shark, Sphyrna zygaeana, to elucidate the early history of vertebrate complement evolution. Identified genes were; one C1qB, one C1r, one C1s, one MASP-1/-3, one MASP-2, two factor B/C2, one C3, three C4, one C5, one C6, one C7, one C8A, three C8B, one C8G, one C9, two factor I and one S protein. No MBL, ficolin, C1qA or C1qC were found. These results indicate that the lectin, classical, alternative and lytic pathways were established in the common ancestor of jawed vertebrates. In addition to the absence of MBL and ficolin, the MASP transcripts lacked the serine protease domain, suggesting that the lectin pathway was lost in the hammerhead shark lineage. PMID:26987526

  16. The Effect of Cytidine on the Structure and Function of an RNA Ligase Ribozyme

    Science.gov (United States)

    Rogers, Jeff; Joyce, Gerald F.

    2001-01-01

    A cytidine-free ribozyme with RNA ligase activity was obtained by in vitro evolution, starting from a pool of random- sequence RNAs that contained only guanosine, adenosine, and uridine. This ribozyme contains 74 nt and catalyzes formation of a 3',5' -phosphodiester linkage with a catalytic rate of 0.016/min. The RNA adopts a simple secondary structure based on a three-way junction motif, with ligation occurring at the end of a stem region located several nucleotides away from the junction. Cytidine was introduced to the cytidine-free ribozyme in a combinatorial fashion and additional rounds of in vitro evolution were carried out to allow the molecule to adapt to this added component. The resulting cytidine-containing ribozyme formed a 3',5' linkage with a catalytic rate of 0.32/min. The improved rate of the cytidine-containing ribozyme was the result of 12 mutations, including seven added cytidines, that remodeled the internal bulge loops located adjacent to the three-way junction and stabilized the peripheral stem regions.

  17. Calibration Binaries

    Science.gov (United States)

    Drummond, J.

    2011-09-01

    Two Excel Spreadsheet files are offered to help calibrate telescope or camera image scale and orientation with binary stars for any time. One is a personally selected list of fixed position binaries and binaries with well-determined orbits, and the other contains all binaries with published orbits. Both are derived from the web site of the Washington Double Star Library. The spreadsheets give the position angle and separation of the binaries for any entered time by taking advantage of Excel's built in iteration function to solve Kepler's transcendental equation.

  18. Investigating single and multiple species fisheries management: stock status evaluation of hammerhead (Sphyrna spp.) sharks in the western North Atlantic Ocean and Gulf of Mexico.

    OpenAIRE

    Hayes, Christopher Glenn

    2007-01-01

    Three hammerhead sharks (Sphyrna spp.) are currently managed as part of the large coastal shark complex in the United States. Including multiple species in an assessment ignores the different stock dynamics of each individual species within the complex due to different life histories. This study completed individual assessments of scalloped (S. lewini), great (S. mokarran), and smooth (S. zygaena) hammerhead sharks in the U.S. Atlantic Ocean and Gulf of Mexico. Combined data for all three ...

  19. Application of Implicit Space Mapping in the Design of Hammerhead Filter in Millimeter-Wave Band

    Directory of Open Access Journals (Sweden)

    Fuqun Zhong

    2012-03-01

    Full Text Available In this study, we present advances in microwave and millimeter-wave device modeling exploiting the Space Mapping (SM technology. New SM-based modeling techniques are used that are easy to implement entirely in the Agilent ADS framework. The implicit space mapping algorithm is applied to the design of hammerhead filter in millimeter-wave band. The validity of this method is confirmed by comparison with fullwave EM simulation result and measured data. Based on the proposed method, a filter was designed and fabricated on a substrate with thickness of 0.254 mm and dielectric constant of 2.2. The experimental results show good agreement with simulated results. It is proved that the accuracy can be achieved using the implicit space mapping algorithm, and the design efficiency can be greatly improved.

  20. Great hammerhead sharks swim on their side to reduce transport costs.

    Science.gov (United States)

    Payne, Nicholas L; Iosilevskii, Gil; Barnett, Adam; Fischer, Chris; Graham, Rachel T; Gleiss, Adrian C; Watanabe, Yuuki Y

    2016-01-01

    Animals exhibit various physiological and behavioural strategies for minimizing travel costs. Fins of aquatic animals play key roles in efficient travel and, for sharks, the functions of dorsal and pectoral fins are considered well divided: the former assists propulsion and generates lateral hydrodynamic forces during turns and the latter generates vertical forces that offset sharks' negative buoyancy. Here we show that great hammerhead sharks drastically reconfigure the function of these structures, using an exaggerated dorsal fin to generate lift by swimming rolled on their side. Tagged wild sharks spend up to 90% of time swimming at roll angles between 50° and 75°, and hydrodynamic modelling shows that doing so reduces drag-and in turn, the cost of transport-by around 10% compared with traditional upright swimming. Employment of such a strongly selected feature for such a unique purpose raises interesting questions about evolutionary pathways to hydrodynamic adaptations, and our perception of form and function. PMID:27457414

  1. A novel strategy for selection of allosteric ribozymes yields RiboReporter™ sensors for caffeine and aspartame

    OpenAIRE

    Ferguson, Alicia; Boomer, Ryan M.; Kurz, Markus; Keene, Sara C.; Diener, John L.; Keefe, Anthony D.; Wilson, Charles; Cload, Sharon T.

    2004-01-01

    We have utilized in vitro selection technology to develop allosteric ribozyme sensors that are specific for the small molecule analytes caffeine or aspartame. Caffeine- or aspartame-responsive ribozymes were converted into fluorescence-based RiboReporter™ sensor systems that were able to detect caffeine or aspartame in solution over a concentration range from 0.5 to 5 mM. With read-times as short as 5 min, these caffeine- or aspartame-dependent ribozymes function as highly specific and facile...

  2. Interacting binaries

    CERN Document Server

    Shore, S N; van den Heuvel, EPJ

    1994-01-01

    This volume contains lecture notes presented at the 22nd Advanced Course of the Swiss Society for Astrophysics and Astronomy. The contributors deal with symbiotic stars, cataclysmic variables, massive binaries and X-ray binaries, in an attempt to provide a better understanding of stellar evolution.

  3. The emergence of ribozymes synthesizing membrane components in RNA-based protocells.

    Science.gov (United States)

    Ma, Wentao; Yu, Chunwu; Zhang, Wentao; Zhou, Ping; Hu, Jiming

    2010-03-01

    A significant problem of the origin of life is the emergence of cellular self-replication. In the context of the "RNA world", a crucial concern is how the RNA-based protocells could achieve the ability to produce their own membrane. Here we show, with the aid of a computer simulation, that for these protocells, there would be "immediately" a selection pressure for the emergence of a ribozyme synthesizing membrane components. The ribozyme would promote the enlargement of cellular space and favor the incoming (by permeation) of RNA's precursors, thus benefit the replication of inner RNA, including itself. Via growth and division, protocells containing the ribozyme would achieve superiority and spread in the system, and meanwhile the ribozyme would spread in the system. The present work is inspiring because it suggests that the transition from molecular self-replication to cellular self-replication might have occurred naturally (and necessarily) in the origin of life, leading to the emergence of Darwinian evolution at the cellular level. PMID:19961895

  4. Ribozyme Activity of RNA Nonenzymatically Polymerized from 3 ',5 '-Cyclic GMP

    Czech Academy of Sciences Publication Activity Database

    Pino, S.; Costanzo, G.; Giorgi, A.; Šponer, Jiří; Šponer, Judit E.; Di Mauro, E.

    2013-01-01

    Roč. 15, č. 12 (2013), s. 5362-5383. ISSN 1099-4300 R&D Projects: GA ČR(CZ) GAP208/10/2302; GA MŠk(CZ) ED1.1.00/02.0068 Institutional support: RVO:68081707 Keywords : RNA polymerization * RNA origin * ribozymes Subject RIV: BO - Biophysics Impact factor: 1.564, year: 2013

  5. Wobble pairs of the HDV ribozyme play specific roles in stabilization of active site dynamics

    Czech Academy of Sciences Publication Activity Database

    Sripathi, K.N.; Banáš, P.; Réblová, K.; Šponer, Jiří; Otyepka, M.; Walter, Nils G.

    2015-01-01

    Roč. 17, č. 8 (2015), s. 5887-5900. ISSN 1463-9076 R&D Projects: GA ČR GAP208/12/1878 Institutional support: RVO:68081707 Keywords : HEPATITIS-DELTA-VIRUS * SELF-CLEAVING RIBOZYMES * ACID-BASE CATALYSIS Subject RIV: BO - Biophysics Impact factor: 4.493, year: 2014

  6. Feeding strategy of the night shark (Carcharhinus signatus) and scalloped hammerhead shark (Sphyrna lewini) near seamounts off northeastern Brazil

    OpenAIRE

    Teodoro Vaske Júnior; Carolus Maria Vooren; Rosangela Paula Lessa

    2009-01-01

    A total of 425 stomachs of night shark (Carcharhinus signatus), and 98 stomachs of scalloped hammerhead shark (Sphyrna lewini), from longline and surface gillneters near seamounts off northeastern Brazil, were analysed between 1992 and 1999. Both predators prey upon reef and benthopelagic fishes, migrant cephalopods and deep water crustaceans, showing similar feeding niches (Schoener Index T=0.75). The great prey richness of the diets may reflect the fact that the marine food web for these sp...

  7. Stomach content analysis of juvenile, scalloped hammerhead shark Sphyrna lewini captured off the coast of Mazatlan, Mexico

    OpenAIRE

    Torres Rojas, Yassir E.; Hernández Herrera, Agustin; Galván Magaña, Felipe; Alatorre Ramírez, Vanessa Guadalupe

    2010-01-01

    We quantified the diet of juvenile, scalloped hammerhead shark Sphyrna lewini in the area off Mazatlan, Sinaloa, Mexico, to understand their feeding ecology this shark. The prey species of Sphyrna lewini were identified and quantified from stomach content analysis. In addition, we determined the variations between genders. During two fishing seasons (2000–2001 and 2001–2002), we analyzed 232 stomachs, of which 85% contained food. The trophic spectrum was composed of three species of cephalopo...

  8. Structural and Chemical Basis for Glucosamine 6-Phosphate Binding and Activation of the glmS Ribozyme

    Energy Technology Data Exchange (ETDEWEB)

    Cochrane, J.; Lipchock, S; Smith, K; Strobel, S

    2009-01-01

    The glmS ribozyme is the first naturally occurring catalytic RNA that relies on an exogenous, nonnucleotide cofactor for reactivity. From a biochemical perspective, the glmS ribozyme derived from Bacillus anthracis is the best characterized. However, much of the structural work to date has been done on a variant glmS ribozyme, derived from Thermoanaerobacter tengcongensis. Here we present structures of the B. anthracis glmS ribozyme in states before the activating sugar, glucosamine 6-phosphate (GlcN6P), has bound and after the reaction has occurred. These structures show an active site preorganized to bind GlcN6P that retains some affinity for the sugar even after cleavage of the RNA backbone. A structure of an inactive glmS ribozyme with a mutation distal from the ligand-binding pocket highlights a nucleotide critical to the reaction that does not affect GlcN6P binding. Structures of the glmS ribozyme bound to a naturally occurring inhibitor, glucose 6-phosphate (Glc6P), and a nonnatural activating sugar, mannosamine 6-phosphate (MaN6P), reveal a binding mode similar to that of GlcN6P. Kinetic analyses show a pH dependence of ligand binding that is consistent with titration of the cofactor's phosphate group and support a model in which the major determinant of activity is the sugar amine independent of its stereochemical presentation.

  9. Effects of fasting and refeeding on intestinal cell proliferation and apoptosis in hammerhead shark (Sphyrna lewini

    Directory of Open Access Journals (Sweden)

    Hideya Takahashi

    2014-04-01

    Full Text Available Objective: To examine the effects of fasting and refeeding on intestinal cell proliferation and apoptosis in an opportunistic predator, hammerhead shark (Sphyrna lewini of elasmobranch fishes which are among the earliest known extant groups of vertebrates to have the valvular intestine typical for the primitive species. Methods: Animals were euthanized after 5-10 d of fasting or feeding, or after 10-day fasting and 5-day refeeding. Intestinal apoptosis and cell proliferation were assessed by using oligonucleotide detection assay, terminal deoxynucleotidyl transferase dUTP nick end labeling staining, and immunohistochemistry of proliferating cells nuclear antigen. Results: Plasma levels of cholesterol and glucose were reduced by fasting. Intestinal apoptosis generally decreased during fasting. Numerous apoptotic cells were observed around the tips of the villi, primarily in the epithelium in the fed sharks, whereas fewer labeled nuclei were detected in the epithelium of fasted sharks. Refeeding returned intestinal apoptosis to the level in the fed sharks. Proliferating cells were observed in the epithelium around the troughs of the villi and greater in number in fed sharks, whereas fewer labeled nuclei were detected in fasted sharks. Conclusions: The cell turnover is modified in both intestinal epithelia of the shark and the murines by fasting/feeding, but in opposite directions. The difference may reflect the feeding ecology of the elasmobranchs, primitive intermittent feeders.

  10. Effects of fasting and refeeding on intestinal cell proliferation and apoptosis in hammerhead shark (Sphyrna lewini)

    Institute of Scientific and Technical Information of China (English)

    Hideya Takahashi; Susumu Hyodo; Tsukasa Abe; Chiyo Takagi; Gordon E Grau; Tatsuya Sakamoto

    2014-01-01

    Objective: To examine the effects of fasting and refeeding on intestinal cell proliferation and apoptosis in an opportunistic predator, hammerhead shark (Sphyrna lewini) of elasmobranch fishes which are among the earliest known extant groups of vertebrates to have the valvular intestine typical for the primitive species.Methods:5-day refeeding. Intestinal apoptosis and cell proliferation were assessed by using oligonucleotide detection assay, terminal deoxynucleotidyl transferase dUTP nick end labeling staining, and immunohistochemistry of proliferating cells nuclear antigen.Results:Animals were euthanized after 5-10 d of fasting or feeding, or after 10-day fasting and generally decreased during fasting. Numerous apoptotic cells were observed around the tips of the villi, primarily in the epithelium in the fed sharks, whereas fewer labeled nuclei were detected in the epithelium of fasted sharks. Refeeding returned intestinal apoptosis to the level in the fed sharks. Proliferating cells were observed in the epithelium around the troughs of the villi and greater in number in fed sharks, whereas fewer labeled nuclei were detected in fasted sharks. Plasma levels of cholesterol and glucose were reduced by fasting. Intestinal apoptosis Conclusions: The cell turnover is modified in both intestinal epithelia of the shark and the murines by fasting/feeding, but in opposite directions. The difference may reflect the feeding ecology of the elasmobranchs, primitive intermittent feeders.

  11. RNA-based networks: using RNA aptamers and ribozymes as synthetic genetic devices.

    Science.gov (United States)

    Weigand, Julia E; Wittmann, Alexander; Suess, Beatrix

    2012-01-01

    Within the last few years, a set of synthetic riboswitches has been engineered, which expands the toolbox of genetic regulatory devices. Small molecule binding aptamers have been used for the design of such riboswitches by insertion into untranslated regions of mRNAs, exploiting the fact that upon ligand binding the RNA structure interferes either with translation initiation or pre-mRNA splicing in yeast. In combination with self-cleaving ribozymes, aptamers have been used to modulate RNA stability. In this chapter, we discuss the applicability of different aptamers, ways to identify novel genetic devices, the pros and cons of various insertion sites and the application of allosteric ribozymes. Our expertise help to apply synthetic riboswitches to engineer complex genetic circuits. PMID:22083741

  12. The Hairpin Ribozyme Substrate Binding-domain: A Highly Constrained D-shaped Conformation

    OpenAIRE

    Pinard, Robert; Lambert, Dominic; Heckman, Joyce; Esteban, José A.; Gundlach IV, William; Hampel, Ken J.; Glick, Gary D.; Walter, Nils; Major, François; John M. Burke

    2011-01-01

    The two domains of the hairpin ribozyme-substrate complex, usually depicted as straight structural elements, must interact with one another in order to form an active conformation. Little is known about the internal geometry of the individual domains in an active docked complex. Using various crosslinking and structural approaches in conjunction with molecular modeling (constraint-satisfaction program MC-SYM), we have investigated the conformation of the substrate-binding do...

  13. Bacterial Riboswitches and Ribozymes Potently Activate the Human Innate Immune Sensor PKR.

    Science.gov (United States)

    Hull, Chelsea M; Anmangandla, Ananya; Bevilacqua, Philip C

    2016-04-15

    The innate immune system provides the first line of defense against pathogens through the recognition of nonspecific patterns in RNA to protect the cell in a generalized way. The human RNA-activated protein kinase, PKR, is a dsRNA binding protein and an essential sensor in the innate immune response, which recognizes viral and bacterial pathogens through their RNAs. Upon activation via RNA-dependent autophosphorylation, PKR phosphorylates the eukaryotic initiation factor eIF2α, leading to termination of translation. PKR has a well-characterized role in recognizing viral RNA, where it binds long stretches of double-stranded RNA nonsequence specifically to promote activation; however, the mechanism by which bacterial RNA activates PKR and the mode by which self RNA avoids activating PKR are unknown. We characterized activation of PKR by three functional bacterial RNAs with pseudoknots and extensive tertiary structure: the cyclic di-GMP riboswitch, the glmS riboswitch-ribozyme, and the twister ribozyme, two of which are ligand-activated. These RNAs were found to activate PKR with comparable potency to long dsRNA. Enzymatic structure mapping in the absence and presence of PKR reveals a clear PKR footprint and provides a structural basis for how these bacterial RNAs activate PKR. In the case of the cyclic di-GMP riboswitch and the glmS riboswitch-ribozyme, PKR appears to dimerize on the peripheral double-stranded regions of the native RNA tertiary structure. Overall, these results provide new insights into how PKR acts as an innate immune signaling protein for the presence of bacteria and suggest a reason for the apparent absence of protein-free riboswitches and ribozymes in the human genome. PMID:27011290

  14. Disparate HDV ribozyme crystal structures represent intermediates on a rugged free-energy landscape

    Czech Academy of Sciences Publication Activity Database

    Sripathi, K.N.; Tay, W.W.; Banáš, P.; Otyepka, M.; Šponer, Jiří; Walter, N. G.

    2014-01-01

    Roč. 20, č. 7 (2014), s. 1112-1128. ISSN 1355-8382 R&D Projects: GA MŠk(CZ) ED1.1.00/02.0068; GA ČR(CZ) GAP208/12/1878 Institutional support: RVO:68081707 Keywords : time-resolved FRET * steady-state FRET * small ribozyme Subject RIV: BO - Biophysics Impact factor: 4.936, year: 2014

  15. Nucleotide synthetase ribozymes may have emerged first in the RNA world

    OpenAIRE

    Ma, Wentao; Yu, Chunwu; Zhang, Wentao; Hu, Jiming

    2007-01-01

    Though the “RNA world” hypothesis has gained a central role in ideas concerning the origin of life, the scenario concerning its emergence remains uncertain. It has been speculated that the first scene may have been the emergence of a template-dependent RNA synthetase ribozyme, which catalyzed its own replication: thus, “RNA replicase.” However, the speculation remains uncertain, primarily because of the large sequence length requirement of such a replicase and the lack of a convincing mechani...

  16. Gene silencing of HIV chemokine receptors using ribozymes and single-stranded antisense RNA.

    Science.gov (United States)

    Qureshi, Amer; Zheng, Richard; Parlett, Terry; Shi, Xiaoju; Balaraman, Priyadhashini; Cheloufi, Sihem; Murphy, Brendan; Guntermann, Christine; Eagles, Peter

    2006-03-01

    The chemokine receptors CXCR4 and CCR5 are required for HIV-1 to enter cells, and the progression of HIV-1 infection to AIDS involves a switch in the co-receptor usage of the virus from CCR5 to CXCR4. These receptors therefore make attractive candidates for therapeutic intervention, and we have investigated the silencing of their genes by using ribozymes and single-stranded antisense RNAs. In the present study, we demonstrate using ribozymes that a depletion of CXCR4 and CCR5 mRNAs can be achieved simultaneously in human PBMCs (peripheral blood mononuclear cells), cells commonly used by the virus for infection and replication. Ribozyme activity leads to an inhibition of the cell-surface expression of both CCR5 and CXCR4, resulting in a significant inhibition of HIV-1 replication when PBMCs are challenged with the virus. In addition, we show that small single-stranded antisense RNAs can also be used to silence CCR5 and CXCR4 genes when delivered to PBMCs. This silencing is caused by selective degradation of receptor mRNAs. PMID:16293105

  17. Magnesium-Dependent Active-Site Conformational Selection in the Diels-Alderase Ribozyme

    Energy Technology Data Exchange (ETDEWEB)

    Berezniak, Tomasz [University of Heidelberg; Zahran, Mai [ORNL; Imhof, Petra [University of Heidelberg; Jaeschke, Andres [Free University of Berlin; Smith, Jeremy C [ORNL

    2010-10-01

    The Diels-Alderase ribozyme, an in vitro-evolved ribonucleic acid enzyme, accelerates the formation of carbon-carbon bonds between an anthracene diene and a maleimide dienophile in a [4 + 2] cycloaddition, a reaction with broad application in organic chemistry. Here, the Diels-Alderase ribozyme is examined via molecular dynamics (MD) simulations in both crystalline and aqueous solution environments. The simulations indicate that the catalytic pocket is highly dynamic. At low Mg(2+) ion concentrations, inactive states with the catalytic pocket closed dominate. Stabilization of the enzymatically active, open state of the catalytic pocket requires a high concentration of Mg(2+) ions (e.g., 54 mM), with cations binding to specific phosphate sites on the backbone of the residues bridging the opposite strands of the pocket. The free energy profile for pocket opening at high Mg(2+) cation concentration exhibits a double minimum, with a barrier to opening of approximately 5.5 kJ/mol and the closed state approximately 3 kJ/mol lower than the open state. Selection of the open state on substrate binding leads to the catalytic activity of the ribozyme. The simulation results explain structurally the experimental observation that full catalytic activity depends on the Mg(2+) ion concentration

  18. The structure of the isolated, central hairpin of the HDV antigenomic ribozyme: novel structural features and similarity of the loop in the ribozyme and free in solution.

    Science.gov (United States)

    Kolk, M H; Heus, H A; Hilbers, C W

    1997-01-01

    The structure of an RNA hairpin containing a seven-nucleotide loop that is present in the self-cleaving sequence of hepatitis delta virus antigenomic RNA was determined by high resolution NMR spectroscopy. The loop, which is composed of only one purine and six pyrimidines, has a suprisingly stable structure, mainly supported by sugar hydroxyl hydrogen bonds and base-base and base-phosphate stacking interactions. Compared with the structurally well-determined, seven-membered anticodon loop in tRNA, the sharp turn which affects the required 180 degrees change in direction of the sugar-phosphate backbone in the loop is shifted one nucleotide in the 3' direction. This change in direction can be characterized as a reversed U-turn. It is expected that the reversed U-turn may be found frequently in other molecules as well. There is evidence for a new non-Watson-Crick UC base pair formed between the first and the last residue in the loop, while most of the other bases in the loop are pointing outwards making them accessible to solvent. From chemical modification, mutational and photocrosslinking studies, a similar picture develops for the structure of the hairpin in the active ribozyme indicating that the loop structure in the isolated hairpin and in the ribozyme is very similar. PMID:9218809

  19. Inhibition of K562 cell growth and tumor angiogenesis in nude mice by transfection of anti-VEGF hairpin ribozyme gene into the cells

    Institute of Scientific and Technical Information of China (English)

    许文林

    2006-01-01

    Objective To explore the effect of anti-VEGF hairpin ribozyme gene on the tumor cell growth and tumor angiogenesis in nude mice. Methods The recombinant eukaryotic expression plasmid pcDNA-RZ containing anti-VEGF hairpin ribozyme gene and the empty vector plasmid pcDNA were introduced separately into K562 cells

  20. A conformational switch in the DiGIR1 ribozyme involved in release and folding of the downstream I-DirI mRNA

    DEFF Research Database (Denmark)

    Nielsen, Henrik; Einvik, Christer; Lentz, Thomas E;

    2009-01-01

    DiGIR1 is a group I-like cleavage ribozyme found as a structural domain within a nuclear twin-ribozyme group I intron. DiGIR1 catalyzes cleavage by branching at an Internal Processing Site (IPS) leading to formation of a lariat cap at the 5'-end of the 3'-cleavage product. The 3'-cleavage product...

  1. GENE THERAPY USING RETROVIRAL VECTOR OF bcr-abl SPECIFIC MULTI-UNIT RIBOZYMES COULD INHIBIT CML CELL GROWTH AND INDUCE APOPTOSIS

    Institute of Scientific and Technical Information of China (English)

    冯琦; 孙凯; 赵永同; 张涛; 尚振川; 王莎; 王玮; 赵宁; 颜真; 韩苇; 张英起; 孙秉中

    2003-01-01

    Objective: To investigate the in vitro cleavage ability and effects on apoptosis and cell growth of the bcr-abl fusion gene specific multi-unit ribozymes. Methods: Three fusion point specific ribozymes were designed and the multi-unit ribozymes' in vitro transcription vector and retroviral vector were constructed. The in vitro cleavage ability was tested. The retroviral vector was transfected into K562 cell and the effects on proliferation, apoptosis, cell cycle and cell structure were observed. Results: Multi-unit ribozymes had in vitro cleavage efficiency of 70.8%, which was more efficient than single-unit and double-unit ribozymes. Transfection of the retroviral vector of the ribozyme into K562 cells, induced inhibition of cell growth and apoptosis. The incorporation rate of DNA in ribozymes transfected K562 cells was greatly decreased along with time passed, with an inhibition rate of more than 50% after 96 h of transfection. Under FCM, 18.4% of the cells underwent apoptosis 72 h after transfection and more cells were blocked in G phase, with the ratio in S phase greatly decreased (41.9%). Under electron microscope, compaction of nuclear chromatin and apoptosis bodies were observed.Conclusion: Multi-unit ribozymes specific to bcr-abl fusion gene can be used to treat CML and to purge bone marrow for self-grafting.

  2. Binary Planets

    Science.gov (United States)

    Ryan, Keegan; Nakajima, Miki; Stevenson, David J.

    2014-11-01

    Can a bound pair of similar mass terrestrial planets exist? We are interested here in bodies with a mass ratio of ~ 3:1 or less (so Pluto/Charon or Earth/Moon do not qualify) and we do not regard the absence of any such discoveries in the Kepler data set to be significant since the tidal decay and merger of a close binary is prohibitively fast well inside of 1AU. SPH simulations of equal mass “Earths” were carried out to seek an answer to this question, assuming encounters that were only slightly more energetic than parabolic (zero energy). We were interested in whether the collision or near collision of two similar mass bodies would lead to a binary in which the two bodies remain largely intact, effectively a tidal capture hypothesis though with the tidal distortion being very large. Necessarily, the angular momentum of such an encounter will lead to bodies separated by only a few planetary radii if capture occurs. Consistent with previous work, mostly by Canup, we find that most impacts are disruptive, leading to a dominant mass body surrounded by a disk from which a secondary forms whose mass is small compared to the primary, hence not a binary planet by our adopted definition. However, larger impact parameter “kissing” collisions were found to produce binaries because the dissipation upon first encounter was sufficient to provide a bound orbit that was then rung down by tides to an end state where the planets are only a few planetary radii apart. The long computational times for these simulation make it difficult to fully map the phase space of encounters for which this outcome is likely but the indications are that the probability is not vanishingly small and since planetary encounters are a plausible part of planet formation, we expect binary planets to exist and be a non-negligible fraction of the larger orbital radius exoplanets awaiting discovery.

  3. Demographic processes underlying subtle patterns of population structure in the scalloped hammerhead shark, Sphyrna lewini.

    Directory of Open Access Journals (Sweden)

    Holly A Nance

    Full Text Available Genetic diversity (θ, effective population size (N(e, and contemporary levels of gene flow are important parameters to estimate for species of conservation concern, such as the globally endangered scalloped hammerhead shark, Sphyrna lewini. Therefore, we have reconstructed the demographic history of S. lewini across its Eastern Pacific (EP range by applying classical and coalescent population genetic methods to a combination of 15 microsatellite loci and mtDNA control region sequences. In addition to significant population genetic structure and isolation-by-distance among seven coastal sites between central Mexico and Ecuador, the analyses revealed that all populations have experienced a bottleneck and that all current values of θ are at least an order of magnitude smaller than ancestral θ, indicating large decreases in N(e (θ = 4N(eμ, where μ is the mutation rate. Application of the isolation-with-migration (IM model showed modest but significant genetic connectivity between most sampled sites (point estimates of Nm = 0.1-16.7, with divergence times (t among all populations significantly greater than zero. Using a conservative (i.e., slow fossil-based taxon-specific phylogenetic calibration for mtDNA mutation rates, posterior probability distributions (PPDs for the onset of the decline in N(e predate modern fishing in this region. The cause of decline over the last several thousand years is unknown but is highly atypical as a post-glacial demographic history. Regardless of the cause, our data and analyses suggest that S. lewini was far more abundant throughout the EP in the past than at present.

  4. Thiosulfate-Hydrogen Peroxide Redox Oscillator as pH Driver for Ribozyme Activity in the RNA World

    Science.gov (United States)

    Ball, Rowena; Brindley, John

    2016-03-01

    The RNA world of more than 3.7 billion years ago may have drawn on thermal and pH oscillations set up by the oxidation of thiosulfate by hydrogen peroxide (the THP oscillator) as a power source to drive replication. Since this primordial RNA also must have developed enzyme functionalities, in this work we examine the responses of two simple ribozymes to a THP periodic drive, using experimental rate and thermochemical data in a dynamical model for the coupled, self-consistent evolution of all reactants and intermediates. The resulting time traces show that ribozyme performance can be enhanced under pH cycling, and that thermal cycling may have been necessary to achieve large performance gains. We discuss three important ways in which the dynamic hydrogen peroxide medium may have acted as an agent for development of the RNA world towards a cellular world: proton gradients, resolution of the ribozyme versus replication paradox, and vesicle formation.

  5. Short-term sequence evolution and vertical inheritance of the Naegleria twin-ribozyme group I intron

    Directory of Open Access Journals (Sweden)

    De Jonckheere Johan F

    2006-05-01

    Full Text Available Abstract Background Ribosomal DNA of several species of the free-living Naegleria amoeba harbors an optional group I intron within the nuclear small subunit ribosomal RNA gene. The intron (Nae.S516 has a complex organization of two ribozyme domains (NaGIR1 and NaGIR2 and a homing endonuclease gene (NaHEG. NaGIR2 is responsible for intron excision, exon ligation, and full-length intron RNA circularization, reactions typical for nuclear group I intron ribozymes. NaGIR1, however, is essential for NaHEG expression by generating the 5' end of the homing endonuclease messenger RNA. Interestingly, this unusual class of ribozyme adds a lariat-cap at the mRNA. Results To elucidate the evolutionary history of the Nae.S516 twin-ribozyme introns we have analyzed 13 natural variants present in distinct Naegleria isolates. Structural variabilities were noted within both the ribozyme domains and provide strong comparative support to the intron secondary structure. One of the introns, present in N. martinezi NG872, contains hallmarks of a degenerated NaHEG. Phylogenetic analyses performed on separate data sets representing NaGIR1, NaGIR2, NaHEG, and ITS1-5.8S-ITS2 ribosomal DNA are consistent with an overall vertical inheritance pattern of the intron within the Naegleria genus. Conclusion The Nae.S516 twin-ribozyme intron was gained early in the Naegleria evolution with subsequent vertical inheritance. The intron was lost in the majority of isolates (70%, leaving a widespread but scattered distribution pattern. Why the apparent asexual Naegleria amoebae harbors active intron homing endonucleases, dependent on sexual reproduction for its function, remains a puzzle.

  6. Flanking sequences with an essential role in hydrolysis of a self-cleaving group I-like ribozyme

    DEFF Research Database (Denmark)

    Einvik, C; Nielsen, Henrik; Nour, R; Johansen, S

    2000-01-01

    that identify RNA elements critical for hydrolysis outside the DiGIR1 ribozyme core moiety. Results from deletion analysis, disruption/compensation mutagenesis and RNA structure probing analysis all support the existence of two new segments, named P2 and P2.1, involved in the hydrolysis of DiGIR1....... Significant decreases in the hydrolysis rate, k (obs), were observed in disruption mutants involving both segments. These effects were restored by compensatory base pairing mutants. The possible role of P2 is to tether the ribozyme core, whereas P2.1 appears to be more directly involved in catalysis....

  7. Molecular dynamics simulations of hepatitis delta virus and hairpin ribozymes: Distinct strategies to use pockets of deep electrostatic potential minima

    Czech Academy of Sciences Publication Activity Database

    Šponer, Jiří; Krasovská, Maryna V.; Réblová, Kamila; Šefčíková, J.; Rhodes, M.M.; Walter, N.G.

    Albany, 2007. s. 693. ISSN 0739-1102. [The 15th Conversation. 19.06.2007-23.06.2007, Albany] Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702 Keywords : delta virus * ribozymes Subject RIV: BO - Biophysics

  8. Analysis of the tertiary structure of the ribonuclease P ribozyme-substrate complex by site-specific photoaffinity crosslinking.

    Science.gov (United States)

    Harris, M E; Kazantsev, A V; Chen, J L; Pace, N R

    1997-06-01

    Bacterial ribonuclease P (RNase P), an endonuclease involved in tRNA maturation, is a ribonucleoprotein containing a catalytic RNA. The secondary structure of this ribozyme is well-established, and a low-resolution model of the three-dimensional structure of the ribozyme-substrate complex has been proposed based on site-specific crosslinking and phylogenetic comparative data [Harris ME et al., 1994 EMBO J 13:3953-3963]. However, several substructures of that model were poorly constrained by the available data. In the present analysis, additional constraints between elements within the Escherichia coli RNase P RNA-pre-tRNA complex were determined by intra- and intermolecular crosslinking experiments. Circularly permuted RNase P RNAs were used to position an azidophenacyl photoactive crosslinking agent specifically at strategic sites within the ribozyme-substrate complex. Crosslink sites were mapped by primer extension and confirmed by analysis of the mobility of the crosslinked RNA lariats on denaturing acrylamide gels relative to circular and linear RNA standards. Crosslinked species generally retained significant catalytic activity, indicating that the results reflect the native ribozyme structure. The crosslinking results support the general configuration of the structure model and predicate new positions and orientations for helices that were previously poorly constrained by the data set. The expanded library of crosslinking constraints was used, together with secondary and tertiary structure identified by phylogenetic sequence comparisons, to refine significantly the model of RNase P RNA with bound substrate pre-tRNA. The crosslinking results and data from chemical-modification and mutational studies are discussed in the context of the current structural perspective on this ribozyme. PMID:9174092

  9. 破碎机锤头铸造成型数值模拟%Numerical Simulation of Casting Process for Crusher Hammerhead

    Institute of Scientific and Technical Information of China (English)

    易克传; 乔印虎

    2012-01-01

    锤击式破碎机锤头在使用中受交变力的作用,极易磨损乃至失效破坏.在有限元应力分析基础上,找出极易发生破坏的部位.通过材料的合理选择,铸造工艺的合理设计,并进行锤头铸造模拟,确保了铸件质量,以便延长锤头使用寿命,提高设计效率.%Crusher hammerhead is exerted force of alternate change in working. Fatigue loads can lead to damage and eventually to failures of hammer. Based on the finite element stress analysis, the site of easy damage was identified. Through the choice of proper material, the design of rational casting process and simulation of casting process for hammerhead, the quality of castings was ensured to extend the hammerhead life and to improve design efficiency.

  10. Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression

    Science.gov (United States)

    Shen, Shensi; Rodrigo, Guillermo; Prakash, Satya; Majer, Eszter; Landrain, Thomas E.; Kirov, Boris; Daròs, José-Antonio; Jaramillo, Alfonso

    2015-01-01

    Organisms have different circuitries that allow converting signal molecule levels to changes in gene expression. An important challenge in synthetic biology involves the de novo design of RNA modules enabling dynamic signal processing in live cells. This requires a scalable methodology for sensing, transmission, and actuation, which could be assembled into larger signaling networks. Here, we present a biochemical strategy to design RNA-mediated signal transduction cascades able to sense small molecules and small RNAs. We design switchable functional RNA domains by using strand-displacement techniques. We experimentally characterize the molecular mechanism underlying our synthetic RNA signaling cascades, show the ability to regulate gene expression with transduced RNA signals, and describe the signal processing response of our systems to periodic forcing in single live cells. The engineered systems integrate RNA–RNA interaction with available ribozyme and aptamer elements, providing new ways to engineer arbitrary complex gene circuits. PMID:25916845

  11. Inducible Expression and Splicing of Candida Group Ⅰ Ribozyme in E.coli

    Institute of Scientific and Technical Information of China (English)

    SHANG Yuan; WANG Chen; ZHANG Yi

    2005-01-01

    The Ca. LSU intron flanking a 129 bp exon upstream and a 100 bp exon downstream was inserted into the lacZ gene on pRS426 to transform E. coli. Northern blot analysis and RT-PCR showed that splicing of Ca. LSU in E.coli is efficient upon inducible expression of the precursor RNA. In contrast, co-transcriptional self-splicing of the intron in vitro is much less active. Therefore, this E. coli splicing system can be used as a better model to investigate the effect of the ribozyme inhibitors on Ca. LSU splicing in living cell. We examined the effects of neomycin sulfate and pentamidine on Ca. LSU splicing in E. coli, and found that these drugs does-dependently inhibit the intron splicing.However, heomycin is more potent than pentamidine in this action.

  12. Chemical Feasibility of the General Acid/Base Mechanism of glmS Ribozyme Self-Cleavage

    Czech Academy of Sciences Publication Activity Database

    Dubecký, M.; Walter, Nils G.; Šponer, Jiří; Otyepka, M.; Banáš, Pavel

    2015-01-01

    Roč. 103, č. 10 (2015), s. 550-562. ISSN 0006-3525 R&D Projects: GA MŠk(CZ) ED1.1.00/02.0068; GA ČR(CZ) GAP208/12/1878 Institutional support: RVO:68081707 Keywords : MOLECULAR-DYNAMICS SIMULATIONS * ACTIVE-SITE GUANINE * HAIRPIN RIBOZYME Subject RIV: BO - Biophysics Impact factor: 2.385, year: 2014

  13. Comparison of ab Initio, DFT, and Semiempirical QM/MM Approaches for Description of Catalytic Mechanism of Hairpin Ribozyme

    Czech Academy of Sciences Publication Activity Database

    Mlýnský, V.; Banáš, P.; Šponer, Jiří; van der Kamp, M.W.; Mulholland, A.J.; Otyepka, M.

    2014-01-01

    Roč. 10, č. 4 (2014), s. 1608-1622. ISSN 1549-9618 R&D Projects: GA ČR(CZ) GAP208/12/1878; GA ČR(CZ) GBP301/11/P558; GA MŠk(CZ) ED1.1.00/02.0068 Institutional support: RVO:68081707 Keywords : MOLECULAR-DYNAMICS SIMULATIONS * DELTA-VIRUS RIBOZYME * ACID- BASE CATALYSIS Subject RIV: BO - Biophysics Impact factor: 5.498, year: 2014

  14. Induction of wild-type p53 activity in human cancer cells by ribozymes that repair mutant p53 transcripts

    OpenAIRE

    Watanabe, Takashi; Sullenger, Bruce A

    2000-01-01

    Several groups have attempted to develop gene therapy strategies to treat cancer via introduction of the wild-type (wt) p53 cDNA into cancer cells. Unfortunately, these approaches do not result in regulated expression of the p53 gene and do not reduce expression of the mutant p53 that is overexpressed in cancerous cells. These shortcomings may greatly limit the utility of this gene replacement approach. We describe an alternative strategy with trans-splicing ribozymes that can simultaneously ...

  15. Tailing cDNAs with terminal deoxynucleotidyl transferase in RT-PCR assays to identify ribozyme cleavage products.

    OpenAIRE

    Albuquerque-Silva, J; Houard, S.; Bollen, A.

    1998-01-01

    Polytailing a cDNA with terminal deoxynucleotidyltransferase (TdT) results in the addition of a homopolymeric sequence at its 3'-end. Here we describe the use of tailing in competitive RT-PCR assays to evaluate cleavage efficiency of ribozymes. Using a system that perfectly mimics intracellular cleavage, we were able to detect as few as 1% of cleaved moieties. Furthermore, employing primers overlapping the junction between tails and the cleaved RNA moiety in non-competitive assays, the sensit...

  16. Chemical feasibility of the general acid/base mechanism of glmS ribozyme self-cleavage.

    Science.gov (United States)

    Dubecký, Matúš; Walter, Nils G; Šponer, Jiří; Otyepka, Michal; Banáš, Pavel

    2015-10-01

    In numerous Gram-positive bacteria, the glmS ribozyme or catalytic riboswitch regulates the expression of glucosamine-6-phosphate (GlcN6P) synthase via site-specific cleavage of its sugar-phosphate backbone in response to GlcN6P ligand binding. Biochemical data have suggested a crucial catalytic role for an active site guanine (G40 in Thermoanaerobacter tengcongensis, G33 in Bacillus anthracis). We used hybrid quantum chemical/molecular mechanical (QM/MM) calculations to probe the mechanism where G40 is deprotonated and acts as a general base. The calculations suggest that the deprotonated guanine G40(-) is sufficiently reactive to overcome the thermodynamic penalty arising from its rare protonation state, and thus is able to activate the A-1(2'-OH) group toward nucleophilic attack on the adjacent backbone. Furthermore, deprotonation of A-1(2'-OH) and nucleophilic attack are predicted to occur as separate steps, where activation of A-1(2'-OH) precedes nucleophilic attack. Conversely, the transition state associated with the rate-determining step corresponds to concurrent nucleophilic attack and protonation of the G1(O5') leaving group by the ammonium moiety of the GlcN6P cofactor. Overall, our calculations help to explain the crucial roles of G40 (as a general base) and GlcN6P (as a general acid) during glmS ribozyme self-cleavage. In addition, we show that the QM/MM description of the glmS ribozyme self-cleavage reaction is significantly more sensitive to the size of the QM region and the quality of the QM-MM coupling than that of other small ribozymes. PMID:25858644

  17. Binary effectivity rules

    DEFF Research Database (Denmark)

    Keiding, Hans; Peleg, Bezalel

    2006-01-01

    Abstract  A social choice rule (SCR) is a collection of social choice correspondences, one for each agenda. An effectivity rule is a collection of effectivity functions, one for each agenda. We prove that every monotonic and superadditive effectivity rule is the effectivity rule of some SCR. A SCR...... is binary if it is rationalized by an acyclic binary relation. The foregoing result motivates our definition of a binary effectivity rule as the effectivity rule of some binary SCR. A binary SCR is regular if it satisfies unanimity, monotonicity, and independence of infeasible alternatives. A binary...... effectivity rule is regular if it is the effectivity rule of some regular binary SCR. We characterize completely the family of regular binary effectivity rules. Quite surprisingly, intrinsically defined von Neumann-Morgenstern solutions play an important role in this characterization...

  18. Eclipsing binaries in open clusters

    DEFF Research Database (Denmark)

    Southworth, John; Clausen, J.V.

    Stars: fundamental parameters - Stars : binaries : eclipsing - Stars: Binaries: spectroscopic - Open clusters and ass. : general Udgivelsesdato: 5 August......Stars: fundamental parameters - Stars : binaries : eclipsing - Stars: Binaries: spectroscopic - Open clusters and ass. : general Udgivelsesdato: 5 August...

  19. PHOEBE: PHysics Of Eclipsing BinariEs

    Science.gov (United States)

    Prsa, Andrej; Matijevic, Gal; Latkovic, Olivera; Vilardell, Francesc; Wils, Patrick

    2011-06-01

    PHOEBE (PHysics Of Eclipsing BinariEs) is a modeling package for eclipsing binary stars, built on top of the widely used WD program (Wilson & Devinney 1971). This introductory paper overviews most important scientific extensions (incorporating observational spectra of eclipsing binaries into the solution-seeking process, extracting individual temperatures from observed color indices, main-sequence constraining and proper treatment of the reddening), numerical innovations (suggested improvements to WD's Differential Corrections method, the new Nelder & Mead's downhill Simplex method) and technical aspects (back-end scripter structure, graphical user interface). While PHOEBE retains 100% WD compatibility, its add-ons are a powerful way to enhance WD by encompassing even more physics and solution reliability.

  20. Crystallization and preliminary diffraction analysis of a group I ribozyme from bacteriophage Twort

    International Nuclear Information System (INIS)

    A group I self-splicing RNA has been synthesized and cocrystallized with a four-nucleotide product RNA. Iodination of the product RNA produces a heavy-atom derivative suitable for structure determination. Group I introns are catalytic RNAs that are capable of performing a variety of phosphotransesterification reactions including self-splicing and RNA cleavage. The reactions are efficient, accurate and dependent only on the presence of guanosine-nucleotide substrate and sufficient magnesium ion to stabilize the structure of the RNA. To understand how the group I intron active-site facilitates catalysis, crystals of a 242-nucleotide ribozyme bound to a four-nucleotide product RNA have been produced that diffract to 3.6 Å resolution. The space group of these crystals is I212121 and the unit-cell parameters are a = 94.6, b = 141.0, c = 210.9 Å. A single heavy-atom derivative has been synthesized by covalent modification of the product RNA with iodine

  1. Encounters of binaries

    International Nuclear Information System (INIS)

    Numerical integrations of encounters of pairs of binaries have been used to study the class of interactions, called fly-bys, in which the two-binary configuration survives. It is shown that these typically weak interactions can be treated by means of a first-order perturbation theory. A simple simulation model for obtaining the energy transfer rate between various degrees of freedom has been constructed. The model was employed to estimate the additional energy transfer arising from impact parameters larger than those used in the numerical experiments. In the hard binary limit the total energy transfer caused by binary-binary encounters is dominated by the collisional interactions in which the two-binary configuration is destroyed. (author)

  2. Global phylogeography with mixed-marker analysis reveals male-mediated dispersal in the endangered scalloped hammerhead shark (Sphyrna lewini.

    Directory of Open Access Journals (Sweden)

    Toby S Daly-Engel

    Full Text Available BACKGROUND: The scalloped hammerhead shark, Sphyrna lewini, is a large endangered predator with a circumglobal distribution, observed in the open ocean but linked ontogenetically to coastal embayments for parturition and juvenile development. A previous survey of maternal (mtDNA markers demonstrated strong genetic partitioning overall (global Φ(ST = 0.749 and significant population separations across oceans and between discontinuous continental coastlines. METHODOLOGY/PRINCIPAL FINDINGS: We surveyed the same global range with increased sample coverage (N = 403 and 13 microsatellite loci to assess the male contribution to dispersal and population structure. Biparentally inherited microsatellites reveal low or absent genetic structure across ocean basins and global genetic differentiation (F(ST = 0.035 over an order of magnitude lower than the corresponding measures for maternal mtDNA lineages (Φ(ST = 0.749. Nuclear allelic richness and heterozygosity are high throughout the Indo-Pacific, while genetic structure is low. In contrast, allelic diversity is low while population structure is higher for populations at the ends of the range in the West Atlantic and East Pacific. CONCLUSIONS/SIGNIFICANCE: These data are consistent with the proposed Indo-Pacific center of origin for S. lewini, and indicate that females are philopatric or adhere to coastal habitats while males facilitate gene flow across oceanic expanses. This study includes the largest sampling effort and the most molecular loci ever used to survey the complete range of a large oceanic predator, and findings emphasize the importance of incorporating mixed-marker analysis into stock assessments of threatened and endangered shark species.

  3. Feeding strategy of the night shark (Carcharhinus signatus and scalloped hammerhead shark (Sphyrna lewini near seamounts off northeastern Brazil

    Directory of Open Access Journals (Sweden)

    Teodoro Vaske Júnior

    2009-06-01

    Full Text Available A total of 425 stomachs of night shark (Carcharhinus signatus, and 98 stomachs of scalloped hammerhead shark (Sphyrna lewini, from longline and surface gillneters near seamounts off northeastern Brazil, were analysed between 1992 and 1999. Both predators prey upon reef and benthopelagic fishes, migrant cephalopods and deep water crustaceans, showing similar feeding niches (Schoener Index T=0.75. The great prey richness of the diets may reflect the fact that the marine food web for these species is very extensive in this region. Due to the concentration for feeding of both predators in the seamounts, intense fisheries should be monitored to avoid localised depletions.Um total de 425 estômagos de tubarão-toninha (Carcharhinus signatus e 98 estômagos de tubarão-martelo-entalhado (Sphyrna lewini, oriundos da pesca de espinhel e pesca com rede de emalhe nas adjacências dos bancos oceânicos do nordeste do Brasil, foi analisado entre 1992 e 1999. Ambas as espécies predam sobre peixes bento-pelágicos e recifais, cefalópodes migradores, e crustáceos de águas profundas, apresentando nichos alimentares similares (Índice de Schoener T=0.75. A alta riqueza de presas nas dietas deve ser um reflexo de que as teias alimentares para estas espécies são consideravelmente longas nessa região. Devido à concentração alimentar de ambos predadores nos bancos oceânicos, a pescaria intensa deve ser monitorada para evitar depleções locais.

  4. The wide expansion of hepatitis delta virus-like ribozymes throughout trypanosomatid genomes is linked to the spreading of L1Tc/ingi clade mobile elements

    OpenAIRE

    Francisco J. Sánchez-Luque; López López, Manuel Carlos; Carreira, Patricia E; Alonso, Carlos; Thomas, María Carmen

    2014-01-01

    Background Hepatitis Delta Virus (HDV)-like ribozymes have recently been found in many mobile elements in which they take part in a mechanism that releases intermediate RNAs from cellular co-transcripts. L1Tc in Trypanosoma cruzi is one of the elements in which such a ribozyme is located. It lies in the so-called Pr77-hallmark, a conserved region shared by retrotransposons belonging to the trypanosomatid L1Tc/ingi clade. The wide distribution of the Pr77-hallmark detected in trypanosomatid re...

  5. Interacting binary stars

    CERN Document Server

    Sahade, Jorge; Ter Haar, D

    1978-01-01

    Interacting Binary Stars deals with the development, ideas, and problems in the study of interacting binary stars. The book consolidates the information that is scattered over many publications and papers and gives an account of important discoveries with relevant historical background. Chapters are devoted to the presentation and discussion of the different facets of the field, such as historical account of the development in the field of study of binary stars; the Roche equipotential surfaces; methods and techniques in space astronomy; and enumeration of binary star systems that are studied

  6. Stabilization of ribozyme-like cis-noncoding rRNAs induces apoptotic and nonapoptotic death in lung cells

    OpenAIRE

    Gee, M; Gu, Y.; Fields, J R; Shiao, Y-H

    2012-01-01

    Bidirectional non-protein-coding RNAs are ubiquitously transcribed from the genome. Convergent sense and antisense transcripts may regulate each other. Here, we examined the convergent cis-noncoding rRNAs (nc-rRNAs) in A5 and E9 lung cancer models. Sense nc-rRNAs extending from rDNA intergenic region to internal transcribed spacer of around 10 kb in length were identified. nc-rRNAs in sense direction exhibited in vitro characteristics of ribozymes, namely, degradation upon incubation with MgC...

  7. A Novel Vector for Abundant Expression of Antisense RNA, Triplex-forming RNA and Ribozyme in vivo

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    For abundant expression of antisense RNA, triplex-forming RNA and Ribozyme in vivo, a novel vector pBSKneorU6' was constructed by PCR cloning. This vector contains the intact human snRNA U6 gene expression unit, yet replacing the 61-nt-sequence in the middle of U6 snRNA coding region with three restriction enzyme sites. Hela nuclear extract in vitro transcription experiments demonstrated that this vector can effectively express U6 mutant RNA. Containing neor at the same time, stably transfected pBSKneorU6' can be selected easily.

  8. Studies of viomycin, an anti-tuberculosis antibiotic: copper(ii) coordination, DNA degradation and the impact on delta ribozyme cleavage activity.

    Science.gov (United States)

    Stokowa-Sołtys, K; Barbosa, N A; Kasprowicz, A; Wieczorek, R; Gaggelli, N; Gaggelli, E; Valensin, G; Wrzesiński, J; Ciesiołka, J; Kuliński, T; Szczepanik, W; Jeżowska-Bojczuk, M

    2016-05-17

    Viomycin is a basic peptide antibiotic, which is among the most effective agents against multidrug-resistant tuberculosis. In this paper we provide the characteristics of its acid base properties, coordination preferences towards the Cu(ii) ions, as well as the reactivity of the resulting complexes against plasmid DNA and HDV ribozyme. Careful coordination studies throughout the wide pH range allow for the characterisation of all the Cu(ii)-viomycin complex species. The assignment of proton chemical shifts was achieved by NMR experiments, while the DTF level of theory was applied to support molecular structures of the studied complexes. The experiments with the plasmid DNA reveal that at the physiological levels of hydrogen peroxide the Cu(ii)-viomycin complex is more aggressive against DNA than uncomplexed metal ions. Moreover, the degradation of DNA by viomycin can be carried out without the presence of transition metal ions. In the studies of antigenomic delta ribozyme catalytic activity, viomycin and its complex are shown to modulate the ribozyme functioning. The molecular modelling approach allows the indication of two different locations of viomycin binding sites to the ribozyme. PMID:27143296

  9. Activating the branch-forming splicing pathway by reengineering the ribozyme component of a natural group II intron.

    Science.gov (United States)

    Monachello, Dario; Michel, François; Costa, Maria

    2016-03-01

    When assayed in vitro, group IIC self-splicing introns, which target bacterial Rho-independent transcription terminators, generally fail to yield branched products during splicing despite their possessing a seemingly normal branchpoint. Starting with intron O.i.I1 from Oceanobacillus iheyensis, whose crystallographically determined structure lacks branchpoint-containing domain VI, we attempted to determine what makes this intron unfit for in vitro branch formation. A major factor was found to be the length of the helix at the base of domain VI: 4 base pairs (bp) are required for efficient branching, even though a majority of group IIC introns have a 3-bp helix. Equally important for lariat formation is the removal of interactions between ribozyme domains II and VI, which are specific to the second step of splicing. Conversely, mismatching of domain VI and its proposed first-step receptor in subdomain IC1 was found to be detrimental; these data suggest that the intron-encoded protein may promote branch formation partly by modulating the equilibrium between conformations specific to the first and second steps of splicing. As a practical application, we show that by making just two changes to the O.i.I1 ribozyme, it is possible to generate sufficient amounts of lariat intron for the latter to be purified and used in kinetic assays in which folding and reaction are uncoupled. PMID:26769855

  10. Molecular Dynamics Study of Twister Ribozyme: Role of Mg(2+) Ions and the Hydrogen-Bonding Network in the Active Site.

    Science.gov (United States)

    Ucisik, Melek N; Bevilacqua, Philip C; Hammes-Schiffer, Sharon

    2016-07-12

    The recently discovered twister ribozyme is thought to utilize general acid-base catalysis in its self-cleavage mechanism, but the roles of nucleobases and metal ions in the mechanism are unclear. Herein, molecular dynamics simulations of the env22 twister ribozyme are performed to elucidate the structural and equilibrium dynamical properties, as well as to examine the role of Mg(2+) ions and possible candidates for the general base and acid in the self-cleavage mechanism. The active site region and the ends of the pseudoknots were found to be less mobile than other regions of the ribozyme, most likely providing structural stability and possibly facilitating catalysis. A purported catalytic Mg(2+) ion and the closest neighboring Mg(2+) ion remained chelated and relatively immobile throughout the microsecond trajectories, although removal of these Mg(2+) ions did not lead to any significant changes in the structure or equilibrium motions of the ribozyme on the microsecond time scale. In addition, a third metal ion, a Na(+) ion remained close to A1(O5'), the leaving group atom, during the majority of the microsecond trajectories, suggesting that it might stabilize the negative charge on A1(O5') during self-cleavage. The locations of these cations and their interactions with key nucleotides in the active site suggest that they may be catalytically relevant. The P1 stem is partially melted at its top and bottom in the crystal structure and further unwinds in the trajectories. The simulations also revealed an interconnected network comprised of hydrogen-bonding and π-stacking interactions that create a relatively rigid network around the self-cleavage site. The nucleotides involved in this network are among the highly conserved nucleotides in twister ribozymes, suggesting that this interaction network may be important to structure and function. PMID:27295275

  11. The binary proletariat

    OpenAIRE

    Bolt, Nate

    2000-01-01

    In the endless quest to transform itself, capitalism has spawned a new working class. The proletariat was an essential product of the industrial revolution, and the lighter, more efficient capitalism of the digital revolution has created the Binary Proletariat.

  12. Eclipsing Binary Pulsars

    CERN Document Server

    Freire, P C C

    2004-01-01

    The first eclipsing binary pulsar, PSR B1957+20, was discovered in 1987. Since then, 13 other eclipsing low-mass binary pulsars have been found, 12 of these are in globular clusters. In this paper we list the known eclipsing binary pulsars and their properties, with special attention to the eclipsing systems in 47 Tuc. We find that there are two fundamentally different groups of eclipsing binary pulsars; separated by their companion masses. The less massive systems (M_c ~ 0.02 M_sun) are a product of predictable stellar evolution in binary pulsars. The systems with more massive companions (M_c ~ 0.2 M_sun) were formed by exchange encounters in globular clusters, and for that reason are exclusive to those environments. This class of systems can be used to learn about the neutron star recycling fraction in the globular clusters actively forming pulsars. We suggest that most of these binary systems are undetectable at radio wavelengths.

  13. Binary Evolutionary Models

    CERN Document Server

    Han, Z

    2008-01-01

    In this talk, we present the general principles of binary evolution and give two examples. The first example is the formation of subdwarf B stars (sdBs) and their application to the long-standing problem of ultraviolet excess (also known as UV-upturn) in elliptical galaxies. The second is for the progenitors of type Ia supernovae (SNe Ia). We discuss the main binary interactions, i.e., stable Roche lobe overflow (RLOF) and common envelope (CE) evolution, and show evolutionary channels leading to the formation of various binary-related objects. In the first example, we show that the binary model of sdB stars of Han et al. (2002, 2003) can reproduce field sdB stars and their counterparts, extreme horizontal branch (EHB) stars, in globular clusters. By applying the binary model to the study of evolutionary population synthesis, we have obtained an ``a priori'' model for the UV-upturn of elliptical galaxies and showed that the UV-upturn is most likely resulted from binary interactions. This has major implications...

  14. Binary Neutron Star Mergers

    Directory of Open Access Journals (Sweden)

    Joshua A. Faber

    2012-07-01

    Full Text Available We review the current status of studies of the coalescence of binary neutron star systems. We begin with a discussion of the formation channels of merging binaries and we discuss the most recent theoretical predictions for merger rates. Next, we turn to the quasi-equilibrium formalisms that are used to study binaries prior to the merger phase and to generate initial data for fully dynamical simulations. The quasi-equilibrium approximation has played a key role in developing our understanding of the physics of binary coalescence and, in particular, of the orbital instability processes that can drive binaries to merger at the end of their lifetimes. We then turn to the numerical techniques used in dynamical simulations, including relativistic formalisms, (magneto-hydrodynamics, gravitational-wave extraction techniques, and nuclear microphysics treatments. This is followed by a summary of the simulations performed across the field to date, including the most recent results from both fully relativistic and microphysically detailed simulations. Finally, we discuss the likely directions for the field as we transition from the first to the second generation of gravitational-wave interferometers and while supercomputers reach the petascale frontier.

  15. Skewed Binary Search Trees

    DEFF Research Database (Denmark)

    Brodal, Gerth Stølting; Moruz, Gabriel

    2006-01-01

    It is well-known that to minimize the number of comparisons a binary search tree should be perfectly balanced. Previous work has shown that a dominating factor over the running time for a search is the number of cache faults performed, and that an appropriate memory layout of a binary search tree...... can reduce the number of cache faults by several hundred percent. Motivated by the fact that during a search branching to the left or right at a node does not necessarily have the same cost, e.g. because of branch prediction schemes, we in this paper study the class of skewed binary search trees. For...... all nodes in a skewed binary search tree the ratio between the size of the left subtree and the size of the tree is a fixed constant (a ratio of 1/2 gives perfect balanced trees). In this paper we present an experimental study of various memory layouts of static skewed binary search trees, where each...

  16. Synthesis of a bifunctional cytidine derivative and its conjugation to RNA for in vitro selection of a cytidine deaminase ribozyme

    Directory of Open Access Journals (Sweden)

    Nico Rublack

    2014-08-01

    Full Text Available Over the past 20 years, the generation of functional RNAs by in vitro selection has become a standard technique. Apart from aptamers for simple binding of defined ligands, also RNAs for catalysis of chemical reactions have been selected. In the latter case, a key step often is the conjugation of one of the two reactants to the library, requiring suitable strategies for terminal or internal RNA functionalization. With the aim of selecting a ribozyme for deamination of cytidine, we have set up a selection scheme involving the attachment of the cytidine acting as deamination substrate to the 3'-terminus of the RNAs in the library, and library immobilization. Here, we report the synthesis of a bifunctional cytidine derivative suitable for conjugation to RNA and linkage of the conjugated library to a streptavidine-coated surface. Successful conjugation of the cytidine derivative to the 3'-terminus of a model RNA is demonstrated.

  17. 发夹核酶的研究与应用%Study and Application of the Hairpin Ribozyme

    Institute of Scientific and Technical Information of China (English)

    姚杰; 杨克恭; 陈松森

    2001-01-01

    @@核酶(ribozyme)是既能特异识别又能特异切割小分子RNA的核酸内切酶,其本身也是RNA,主要包括发夹核酶、锤头核酶、丁肝病毒、链孢霉属VS和铅依赖性RNA,共同特点是可逆地切割底物RNA的磷酸二酯键,生成5′-OH和2′,3′-环磷酸末端。虽然催化产物相似,但它们的结构和催化机制却是很不相同的。 发夹核酶(hairpin ribozyme)发现于三种不同植物RNA病毒,即烟草环点病毒(to-bacco ringspot virus),菊苣黄色斑点病毒型(chicory yellowmottle virus type)和筷子芥花叶病毒(arabis mosaic virus)。三种发夹核酶分别是这些RNA病毒卫星RNA(satelliteRNA)的负链,它们的英文缩写分别是sTRSV、sCYMVT和sARMV,均为单链RNA,以滚环方式复制[1,2]。目前针对AIDS而人工合成的发夹核酶已成为第一个进入基因治疗临床试验阶段的核酶。

  18. Quantum mechanical/molecular mechanical free energy simulations of the self-cleavage reaction in the hepatitis delta virus ribozyme.

    Science.gov (United States)

    Ganguly, Abir; Thaplyal, Pallavi; Rosta, Edina; Bevilacqua, Philip C; Hammes-Schiffer, Sharon

    2014-01-29

    The hepatitis delta virus (HDV) ribozyme catalyzes a self-cleavage reaction using a combination of nucleobase and metal ion catalysis. Both divalent and monovalent ions can catalyze this reaction, although the rate is slower with monovalent ions alone. Herein, we use quantum mechanical/molecular mechanical (QM/MM) free energy simulations to investigate the mechanism of this ribozyme and to elucidate the roles of the catalytic metal ion. With Mg(2+) at the catalytic site, the self-cleavage mechanism is observed to be concerted with a phosphorane-like transition state and a free energy barrier of ∼13 kcal/mol, consistent with free energy barrier values extrapolated from experimental studies. With Na(+) at the catalytic site, the mechanism is observed to be sequential, passing through a phosphorane intermediate, with free energy barriers of 2-4 kcal/mol for both steps; moreover, proton transfer from the exocyclic amine of protonated C75 to the nonbridging oxygen of the scissile phosphate occurs to stabilize the phosphorane intermediate in the sequential mechanism. To explain the slower rate observed experimentally with monovalent ions, we hypothesize that the activation of the O2' nucleophile by deprotonation and orientation is less favorable with Na(+) ions than with Mg(2+) ions. To explore this hypothesis, we experimentally measure the pKa of O2' by kinetic and NMR methods and find it to be lower in the presence of divalent ions rather than only monovalent ions. The combined theoretical and experimental results indicate that the catalytic Mg(2+) ion may play three key roles: assisting in the activation of the O2' nucleophile, acidifying the general acid C75, and stabilizing the nonbridging oxygen to prevent proton transfer to it. PMID:24383543

  19. Binary Popldation Synthcsis Study

    Institute of Scientific and Technical Information of China (English)

    HAN Zhanwen

    2011-01-01

    Binary population synthesis (BPS), an approach to evolving millions of stars (including binaries) simultaneously, plays a crucial role in our understanding of stellar physics, the structure and evolution of galaxies, and cosmology. We proposed and developed a BPS approach, and used it to investigate the formation of many peculiar stars such as hot subdwarf stars, progenitors of type la supernovae, barium stars, CH stars, planetary nebulae, double white dwarfs, blue stragglers, contact binaries, etc. We also established an evolution population synthesis (EPS) model, the Yunnan Model, which takes into account binary interactions for the first time. We applied our model for the origin of hot subdwarf stars in the study of elliptical galaxies and explained their far-UV radiation.

  20. Binary and Millisecond Pulsars

    Directory of Open Access Journals (Sweden)

    Lorimer Duncan R.

    2008-11-01

    Full Text Available We review the main properties, demographics and applications of binary and millisecond radio pulsars. Our knowledge of these exciting objects has greatly increased in recent years, mainly due to successful surveys which have brought the known pulsar population to over 1800. There are now 83 binary and millisecond pulsars associated with the disk of our Galaxy, and a further 140 pulsars in 26 of the Galactic globular clusters. Recent highlights include the discovery of the young relativistic binary system PSR J1906+0746, a rejuvination in globular cluster pulsar research including growing numbers of pulsars with masses in excess of 1.5M_⊙, a precise measurement of relativistic spin precession in the double pulsar system and a Galactic millisecond pulsar in an eccentric (e = 0.44 orbit around an unevolved companion.

  1. Binary and Millisecond Pulsars

    CERN Document Server

    Lorimer, D R

    2008-01-01

    We review the main properties, demographics and applications of binary and millisecond radio pulsars. Our knowledge of these exciting objects has greatly increased in recent years, mainly due to successful surveys which have brought the known pulsar population to over 1800. There are now 83 binary and millisecond pulsars associated with the disk of our Galaxy, and a further 140 pulsars in 26 of the Galactic globular clusters. Recent highlights include the discovery of the young relativistic binary system PSR J1906+0746, a rejuvination in globular cluster pulsar research including growing numbers of pulsars with masses in excess of 1.5 solar masses, a precise measurement of relativistic spin precession in the double pulsar system and a Galactic millisecond pulsar in an eccentric (e=0.44) orbit around an unevolved companion.

  2. Hypervelocity binary stars: smoking gun of massive binary black holes

    CERN Document Server

    Lu, Youjun; Lin, D N C

    2007-01-01

    The hypervelocity stars recently found in the Galactic halo are expelled from the Galactic center through interactions between binary stars and the central massive black hole or between single stars and a hypothetical massive binary black hole. In this paper, we demonstrate that binary stars can be ejected out of the Galactic center with velocities up to 10^3 km/s, while preserving their integrity, through interactions with a massive binary black hole. Binary stars are unlikely to attain such high velocities via scattering by a single massive black hole or through any other mechanisms. Based on the above theoretical prediction, we propose a search for binary systems among the hypervelocity stars. Discovery of hypervelocity binary stars, even one, is a definitive evidence of the existence of a massive binary black hole in the Galactic center.

  3. Milli-arcsecond Binaries

    CERN Document Server

    Torres, R M; Mioduszewki, A; Rodríguez, L F

    2008-01-01

    As part of an astrometric program, we have used the Very Long Baseline Array to measure the trigonometric parallax of several young stars in the Taurus and Ophiuchus star-forming regions with great accuracy. Additionally, we have obtained an unprecedented sample of high-resolution (~ 1 mas) images of several young stellar systems. These images revealed that about 70% of the stars in our sample are very tight binary stars (with separations of a few mas). Since it is highly unlikely that 70% of all stars are such tight binaries, we argue that selection effects are at work.

  4. Binary Cumulant Varieties

    CERN Document Server

    Sturmfels, Bernd

    2011-01-01

    Algebraic statistics for binary random variables is concerned with highly structured algebraic varieties in the space of 2x2x...x2-tensors. We demonstrate the advantages of representing such varieties in the coordinate system of binary cumulants. Our primary focus lies on hidden subset models. Parametrizations and implicit equations in cumulants are derived for hyperdeterminants, for secant and tangential varieties of Segre varieties, and for certain context-specific independence models. Extending work of Rota and collaborators, we explore the polynomial inequalities satisfied by cumulants.

  5. Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34+ progenitor cells and derivation of HIV-1 resistant T cells and macrophages

    Directory of Open Access Journals (Sweden)

    Rossi John

    2004-12-01

    Full Text Available Abstract Background RNA based antiviral approaches against HIV-1 are among the most promising for long-term gene therapy. These include ribozymes, aptamers (decoys, and small interfering RNAs (siRNAs. Lentiviral vectors are ideal for transduction of such inhibitory RNAs into hematopoietic stem cells due to their ability to transduce non-dividing cells and their relative refractiveness to gene silencing. The objective of this study is to introduce an HIV-1 Tar aptamer either alone or in combination with an anti-CCR5 ribozyme into CD34+ hematopoietic progenitor cells via an HIV-based lentiviral vector to derive viral resistant progeny T cells and macrophages. Results High efficiency and sustained gene transfer into CD34+ cells were achieved with lentiviral vector constructs harboring either Tar decoy or Tar decoy in combination with CCR5 ribozyme. Cells transduced with these constructs differentiated normally into T-lymphocytes in vivo in thy/liv grafts of SCID-hu mice, and into macrophages in vitro in the presence of appropriate growth factors. When challenged in vitro, the differentiated T lymphocytes and macrophages showed marked resistance against HIV-1 infection. Conclusions Viral resistant transgenic T cells and macrophages that express HIV-1 Tar aptamer either alone or in combination with an anti-CCR5 ribozyme could be obtained by lentiviral gene transduction of CD34+ progenitor cells. These results showed for the first time that expression of these anti-HIV-1 transgenes in combination do not interfere with normal thymopoiesis and thus have set the stage for their application in stem cell based gene therapy for HIV/AIDS.

  6. Formation of binary radio pulsars

    International Nuclear Information System (INIS)

    In the framework of the standard scenario of the evolution of massive binary stars a study is made of the formation of final binary systems in which at least one of the components is a neutron star. It is found that about every fortieth radio pulsar must be a member of a close binary system. This is confirmed by observations. Radio pulsars are not formed in wide binary systems, possibly because of the very slow rotation of the presupernova stars

  7. Compressing Binary Decision Diagrams

    DEFF Research Database (Denmark)

    Hansen, Esben Rune; Satti, Srinivasa Rao; Tiedemann, Peter

    The paper introduces a new technique for compressing Binary Decision Diagrams in those cases where random access is not required. Using this technique, compression and decompression can be done in linear time in the size of the BDD and compression will in many cases reduce the size of the BDD to 1...

  8. Equational binary decision diagrams

    NARCIS (Netherlands)

    Groote, J.F.; Pol, J.C. van de

    2000-01-01

    We incorporate equations in binary decision diagrams (BDD). The resulting objects are called EQ-BDDs. A straightforward notion of ordered EQ-BDDs (EQ-OBDD) is defined, and it is proved that each EQ-BDD is logically equivalent to an EQ-OBDD. Moreover, on EQ-OBDDs satisfiability and tautology checkin

  9. Non-Watson Crick base pairs might stabilize RNA structural motifs in ribozymes – A comparative study of group-I intron structures

    Indian Academy of Sciences (India)

    K Chandrasekhar; R Malathi

    2003-09-01

    In recent decades studies on RNA structure and function have gained significance due to discoveries on diversified functions of RNA. A common element for RNA secondary structure formed by series of non-Watson/Watson Crick base pairs, internal loops and pseudoknots have been the highlighting feature of recent structural determination of RNAs. The recent crystal structure of group-I introns has demonstrated that these might constitute RNA structural motifs in ribozymes, playing a crucial role in their enzymatic activity. To understand the functional significance of these non-canonical base pairs in catalytic RNA, we analysed the sequences of group-I introns from nuclear genes. The results suggest that they might form the building blocks of folded RNA motifs which are crucial to the catalytic activity of the ribozyme. The conservation of these, as observed from divergent organisms, argues for the presence of non-canonical base pairs as an important requisite for the structure and enzymatic property of ribozymes by enabling them to carry out functions such as replication, polymerase activity etc. in primordial conditions in the absence of proteins.

  10. Binary MEMS gas sensors

    International Nuclear Information System (INIS)

    A novel sensing mechanism for electrostatic MEMS that employs static bifurcation-based sensing and binary detection is demonstrated. It is implemented as an ethanol vapour sensor that exploits the static pull-in bifurcation. Sensor detection of 5 ppm of ethanol vapour in dry nitrogen, equivalent to a detectable mass of 165 pg, is experimentally demonstrated. Sensor robustness to external disturbances is also demonstrated. A closed-form expression for the sensitivity of statically detected electrostatic MEMS sensors is derived. It is shown that the sensitivity of static bifurcation-based binary electrostatic MEMS sensors represents an upper bound on the sensitivity of static detection for given sensor dimensions and material properties. (paper)

  11. Binary Tetrahedral Flavor Symmetry

    CERN Document Server

    Eby, David A

    2013-01-01

    A study of the T' Model and its variants utilizing Binary Tetrahedral Flavor Symmetry. We begin with a description of the historical context and motivations for this theory, together with some conceptual background for added clarity, and an account of our theory's inception in previous works. Our model endeavors to bridge two categories of particles, leptons and quarks, a unification made possible by the inclusion of additional Higgs particles, shared between the two fermion sectors and creating a single coherent system. This is achieved through the use of the Binary Tetrahedral symmetry group and an investigation of the Tribimaximal symmetry evidenced by neutrinos. Our work details perturbations and extensions of this T' Model as we apply our framework to neutrino mixing, quark mixing, unification, and dark matter. Where possible, we evaluate model predictions against experimental results and find excellent matching with the atmospheric and reactor neutrino mixing angles, an accurate prediction of the Cabibb...

  12. Compressing Binary Decision Diagrams

    CERN Document Server

    Hansen, Esben Rune; Tiedemann, Peter

    2008-01-01

    The paper introduces a new technique for compressing Binary Decision Diagrams in those cases where random access is not required. Using this technique, compression and decompression can be done in linear time in the size of the BDD and compression will in many cases reduce the size of the BDD to 1-2 bits per node. Empirical results for our compression technique are presented, including comparisons with previously introduced techniques, showing that the new technique dominate on all tested instances.

  13. Knocking on FXR's door: the "hammerhead"-structure series of FXR agonists - amphiphilic isoxazoles with potent in vitro and in vivo activities.

    Science.gov (United States)

    Gege, Christian; Kinzel, Olaf; Steeneck, Christoph; Schulz, Andreas; Kremoser, Claus

    2014-01-01

    The Farnesoid X Receptor (FXR) was recently validated in clinical studies using the bile acid analogue Obeticholic Acid (OCA) as an attractive drug target for liver diseases such as Primary Biliary Cirrhosis (PBC) or Non-alcoholic Steatohepatitis (NASH). OCA, however, turned out to induce cholesterol- related side effects upon prolonged treatment and it shows bile acid like pharmacokinetics. The quest for synthetic non-steroidal FXR agonists with general drug likeliness and improved pharmacokinetic and - dynamic properties has started more than a decade ago: The first non-steroidal and selective FXR agonist with decent submicromolar potency, GW4064, was patented in 1998 and published in 2000. Since then, many pharmaceutical companies have taken GW4064 as a structural template for their efforts in identifying novel patentable FXR agonists with the GW-derived trisubstituted isoxazole general structure. However, so far only one compound out of these different series has made it into the early stages of clinical development: The Px-102/Px-104 from Phenex is currently tested in a phase IIa study in patients with Non-Alcoholic Fatty Liver Disease (NAFLD). In this review we try to summarize from the patent and scientific literature the attempts to improve the GW4064 structure into different directions. Furthermore, we suggest directions for further improvements of this special class of synthetic FXR agonists which all display the typical "hammerhead"-conformation in the FXR ligand binding pocket that provides the basis for their impressive in vitro and in vivo potencies. PMID:25388536

  14. Binary-Signal Recovery

    Science.gov (United States)

    Griebeler, Elmer L.

    2011-01-01

    Binary communication through long cables, opto-isolators, isolating transformers, or repeaters can become distorted in characteristic ways. The usual solution is to slow the communication rate, change to a different method, or improve the communication media. It would help if the characteristic distortions could be accommodated at the receiving end to ease the communication problem. The distortions come from loss of the high-frequency content, which adds slopes to the transitions from ones to zeroes and zeroes to ones. This weakens the definition of the ones and zeroes in the time domain. The other major distortion is the reduction of low frequency, which causes the voltage that defines the ones or zeroes to drift out of recognizable range. This development describes a method for recovering a binary data stream from a signal that has been subjected to a loss of both higher-frequency content and low-frequency content that is essential to define the difference between ones and zeroes. The method makes use of the frequency structure of the waveform created by the data stream, and then enhances the characteristics related to the data to reconstruct the binary switching pattern. A major issue is simplicity. The approach taken here is to take the first derivative of the signal and then feed it to a hysteresis switch. This is equivalent in practice to using a non-resonant band pass filter feeding a Schmitt trigger. Obviously, the derivative signal needs to be offset to halfway between the thresholds of the hysteresis switch, and amplified so that the derivatives reliably exceed the thresholds. A transition from a zero to a one is the most substantial, fastest plus movement of voltage, and therefore will create the largest plus first derivative pulse. Since the quiet state of the derivative is sitting between the hysteresis thresholds, the plus pulse exceeds the plus threshold, switching the hysteresis switch plus, which re-establishes the data zero to one transition

  15. Massive Black Hole Binary Evolution

    Directory of Open Access Journals (Sweden)

    Merritt David

    2005-11-01

    Full Text Available Coalescence of binary supermassive black holes (SBHs would constitute the strongest sources of gravitational waves to be observed by LISA. While the formation of binary SBHs during galaxy mergers is almost inevitable, coalescence requires that the separation between binary components first drop by a few orders of magnitude, due presumably to interaction of the binary with stars and gas in a galactic nucleus. This article reviews the observational evidence for binary SBHs and discusses how they would evolve. No completely convincing case of a bound, binary SBH has yet been found, although a handful of systems (e.g. interacting galaxies; remnants of galaxy mergers are now believed to contain two SBHs at projected separations of <~ 1kpc. N-body studies of binary evolution in gas-free galaxies have reached large enough particle numbers to reproduce the slow, “diffusive” refilling of the binary’s loss cone that is believed to characterize binary evolution in real galactic nuclei. While some of the results of these simulations - e.g. the binary hardening rate and eccentricity evolution - are strongly N-dependent, others - e.g. the “damage” inflicted by the binary on the nucleus - are not. Luminous early-type galaxies often exhibit depleted cores with masses of ~ 1-2 times the mass of their nuclear SBHs, consistent with the predictions of the binary model. Studies of the interaction of massive binaries with gas are still in their infancy, although much progress is expected in the near future. Binary coalescence has a large influence on the spins of SBHs, even for mass ratios as extreme as 10:1, and evidence of spin-flips may have been observed.

  16. Biclustering Sparse Binary Genomic Data

    OpenAIRE

    Van Uitert, M.; Meuleman, W.; Wessels, L. F. A.

    2008-01-01

    Genomic datasets often consist of large, binary, sparse data matrices. In such a dataset, one is often interested in finding contiguous blocks that (mostly) contain ones. This is a biclustering problem, and while many algorithms have been proposed to deal with gene expression data, only two algorithms have been proposed that specifically deal with binary matrices. None of the gene expression biclustering algorithms can handle the large number of zeros in sparse binary matrices. The two propos...

  17. Evolution of Close Binary Systems

    Energy Technology Data Exchange (ETDEWEB)

    Yakut, K; Eggleton, P

    2005-01-24

    We collected data on the masses, radii, etc. of three classes of close binary stars: low-temperature contact binaries (LTCBs), near-contact binaries (NCBs), and detached close binaries (DCBs). They restrict themselves to systems where (1) both components are, at least arguably, near the Main Sequence, (2) the periods are less than a day, and (3) there is both spectroscopic and photometric analysis leading to reasonably reliable data. They discuss the possible evolutionary connections between these three classes, emphasizing the roles played by mass loss and angular momentum loss in rapidly-rotating cool stars.

  18. Report IAU Comm. 42, Close Binary Stars

    OpenAIRE

    Ribas, Ignasi; Scarfe, Colin D.; Torres, Guillermo; Rucinski, Slavek M.; Sion, Edward M.; Richards, Mercedes T.; Niarchos, Panayiotis; Olah, Katalin

    2008-01-01

    Brief summaries are given about (1) close binary research from the perspective of the Bibliography of Close Binaries, (2) low-mass binaries and model discrepancies, (3) W UMa-type binaries, (4) cataclysmic variables, (5) Algol binaries, (6) the oEA stars, (7) effects of binarity on stellar activity.

  19. Low autocorrelation binary sequences

    Science.gov (United States)

    Packebusch, Tom; Mertens, Stephan

    2016-04-01

    Binary sequences with minimal autocorrelations have applications in communication engineering, mathematics and computer science. In statistical physics they appear as groundstates of the Bernasconi model. Finding these sequences is a notoriously hard problem, that so far can be solved only by exhaustive search. We review recent algorithms and present a new algorithm that finds optimal sequences of length N in time O(N {1.73}N). We computed all optimal sequences for N≤slant 66 and all optimal skewsymmetric sequences for N≤slant 119.

  20. Magnetic binary nanofillers

    International Nuclear Information System (INIS)

    Magnetic binary nanofillers containing multiwall carbon nanotubes (MWCNT) and hercynite were synthesized by Chemical Vapor Deposition (CVD) on Fe/AlOOH prepared by the sol-gel method. The catalyst precursor was fired at 450 °C, ground and sifted through different meshes. Two powders were obtained with different particle sizes: sample A (50-75 μm) and sample B (smaller than 50 μm). These powders are composed of iron oxide particles widely dispersed in the non-crystalline matrix of aluminum oxide and they are not ferromagnetic. After reduction process the powders are composed of α-Fe nanoparticles inside hercynite matrix. These nanofillers are composed of hercynite containing α-Fe nanoparticles and MWCNT. The binary magnetic nanofillers were slightly ferromagnetic. The saturation magnetization of the nanofillers depended on the powder particle size. The nanofiller obtained from powder particles in the range 50-75 μm showed a saturation magnetization 36% higher than the one formed from powder particles smaller than 50 μm. The phenomenon is explained in terms of changes in the magnetic environment of the particles as consequence of the presence of MWCNT.

  1. Computing on Binary Strings

    CERN Document Server

    Bu, Tian-Ming; Zhang, Peng

    2011-01-01

    Many problems in Computer Science can be abstracted to the following question: given a set of objects and rules respectively, which new objects can be produced? In the paper, we consider a succinct version of the question: given a set of binary strings and several operations like conjunction and disjunction, which new binary strings can be generated? Although it is a fundamental problem, to the best of our knowledge, the problem hasn't been studied yet. In this paper, an O(m^2n) algorithm is presented to determine whether a string s is representable by a set W, where n is the number of strings in W and each string has the same length m. However, looking for the minimum subset from a set to represent a given string is shown to be NP-hard. In addition, we prove that counting the number of strings representable is #P-complete. But if the operator negation can be used, the number is some power of 2. This di?erence maybe help us understand the problem more profoundly.

  2. General base catalysis for cleavage by the active-site cytosine of the hepatitis delta virus ribozyme: QM/MM calculations establish chemical feasibility

    Czech Academy of Sciences Publication Activity Database

    Banáš, Pavel; Rulíšek, Lubomír; Hánošová, V.; Svozil, Daniel; Walter, N.G.; Šponer, Jiří; Otyepka, Michal

    2008-01-01

    Roč. 112, č. 35 (2008), s. 11177-11187. ISSN 1520-6106 R&D Projects: GA MŠk LC512; GA MŠk(CZ) LC06030; GA AV ČR(CZ) IAA400040802; GA AV ČR 1QS500040581 Grant ostatní: NIH(US) GM62357 Institutional research plan: CEZ:AV0Z40550506; CEZ:AV0Z50040507; CEZ:AV0Z50040702 Keywords : HDV ribozyme * catalysis * QM/MM calculations Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 4.189, year: 2008

  3. Relativistic Binaries in Globular Clusters

    Directory of Open Access Journals (Sweden)

    Benacquista Matthew J.

    2006-02-01

    Full Text Available The galactic population of globular clusters are old, dense star systems, with a typical cluster containing 10^4 - 10^7 stars. As an old population of stars, globular clusters contain many collapsed and degenerate objects. As a dense population of stars, globular clusters are the scene of many interesting close dynamical interactions between stars. These dynamical interactions can alter the evolution of individual stars and can produce tight binary systems containing one or two compact objects. In this review, we discuss the theoretical models of globular cluster evolution and binary evolution, techniques for simulating this evolution which lead to relativistic binaries, and current and possible future observational evidence for this population. Globular cluster evolution will focus on the properties that boost the production of hard binary systems and on the tidal interactions of the galaxy with the cluster, which tend to alter the structure of the globular cluster with time. The interaction of the components of hard binary systems alters the evolution of both bodies and can lead to exotic objects. Direct N-body integrations and Fokker-Planck simulations of the evolution of globular clusters that incorporate tidal interactions and lead to predictions of relativistic binary populations are also discussed. We discuss the current observational evidence for cataclysmic variables, millisecond pulsars, and low-mass X-ray binaries as well as possible future detection of relativistic binaries with gravitational radiation.

  4. Relativistic Binaries in Globular Clusters

    Directory of Open Access Journals (Sweden)

    Matthew J. Benacquista

    2013-03-01

    Full Text Available Galactic globular clusters are old, dense star systems typically containing 10^4 – 10^6 stars. As an old population of stars, globular clusters contain many collapsed and degenerate objects. As a dense population of stars, globular clusters are the scene of many interesting close dynamical interactions between stars. These dynamical interactions can alter the evolution of individual stars and can produce tight binary systems containing one or two compact objects. In this review, we discuss theoretical models of globular cluster evolution and binary evolution, techniques for simulating this evolution that leads to relativistic binaries, and current and possible future observational evidence for this population. Our discussion of globular cluster evolution will focus on the processes that boost the production of tight binary systems and the subsequent interaction of these binaries that can alter the properties of both bodies and can lead to exotic objects. Direct N-body integrations and Fokker–Planck simulations of the evolution of globular clusters that incorporate tidal interactions and lead to predictions of relativistic binary populations are also discussed. We discuss the current observational evidence for cataclysmic variables, millisecond pulsars, and low-mass X-ray binaries as well as possible future detection of relativistic binaries with gravitational radiation.

  5. PERIODIC COMPLEMENTARY BINARY SEQUENCE PAIRS

    Institute of Scientific and Technical Information of China (English)

    XuChengqian; ZhaoXiaoqun

    2002-01-01

    A new set of binary sequences-Periodic Complementary Binary Sequence Pair (PCSP)is proposed .A new class of block design-Difference Family Pair (DFP)is also proposed .The relationship between PCSP and DFP,the properties and exising conditions of PCSP and the recursive constructions for PCSP are given.

  6. PERIODIC COMPLEMENTARY BINARY SEQUENCE PAIRS

    Institute of Scientific and Technical Information of China (English)

    Xu Chengqian; Zhao Xiaoqun

    2002-01-01

    A new set of binary sequences-Periodic Complementary Binary Sequence Pair (PCSP) is proposed. A new class of block design-Difference Family Pair (DFP) is also proposed.The relationship between PCSP and DFP, the properties and existing conditions of PCSP and the recursive constructions for PCSP are given.

  7. Pairing mechanisms for binary stars

    CERN Document Server

    Kouwenhoven, M B N; Goodwin, S P; Zwart, S F Portegies; Kaper, L; 10.1002/asna.200811061

    2008-01-01

    Knowledge of the binary population in stellar groupings provides important information about the outcome of the star forming process in different environments. Binarity is also a key ingredient in stellar population studies and is a prerequisite to calibrate the binary evolution channels. In these proceedings we present an overview of several commonly used methods to pair individual stars into binary systems, which we refer to as the pairing function. Many pairing functions are frequently used by observers and computational astronomers, either for the mathematical convenience, or because they roughly describe the expected outcome of the star forming process. We discuss the consequences of each pairing function for the interpretation of observations and numerical simulations. The binary fraction and mass ratio distribution generally depend strongly on the selection of the range in primary spectral type in a sample. These quantities, when derived from a binary survey with a mass-limited sample of target stars, ...

  8. Planets in Binary Star Systems

    CERN Document Server

    Haghighipour, Nader

    2010-01-01

    The discovery of extrasolar planets over the past decade has had major impacts on our understanding of the formation and dynamical evolution of planetary systems. There are features and characteristics unseen in our solar system and unexplainable by the current theories of planet formation and dynamics. Among these new surprises is the discovery of planets in binary and multiple-star systems. The discovery of such "binary-planetary" systems has confronted astrodynamicists with many new challenges, and has led them to re-examine the theories of planet formation and dynamics. Among these challenges are: How are planets formed in binary star systems? What would be the notion of habitability in such systems? Under what conditions can binary star systems have habitable planets? How will volatiles necessary for life appear on such planets? This volume seeks to gather the current research in the area of planets in binary and multistar systems and to familiarize readers with its associated theoretical and observation...

  9. Towards Physarum Binary Adders

    CERN Document Server

    Jones, Jeff; 10.1016/j.biosystems.2010.04.005

    2010-01-01

    Plasmodium of \\emph{Physarum polycephalum} is a single cell visible by unaided eye. The plasmodium's foraging behaviour is interpreted in terms of computation. Input data is a configuration of nutrients, result of computation is a network of plasmodium's cytoplasmic tubes spanning sources of nutrients. Tsuda et al (2004) experimentally demonstrated that basic logical gates can be implemented in foraging behaviour of the plasmodium. We simplify the original designs of the gates and show --- in computer models --- that the plasmodium is capable for computation of two-input two-output gate $ \\to $ and three-input two-output $ \\to $. We assemble the gates in a binary one-bit adder and demonstrate validity of the design using computer simulation.

  10. Eccentric Binary Millisecond Pulsars

    CERN Document Server

    Freire, Paulo C C

    2009-01-01

    In this paper we review the recent discovery of several millisecond pulsars (MSPs) in eccentric binary systems. Timing these MSPs we were able to estimate (and in one case precisely measure) their masses. These results suggest that, as a class, MSPs have a much wider range of masses (1.3 to > 2 solar masses) than the normal and mildly recycled pulsars found in double neutron star (DNS) systems (1.25 < Mp < 1.44 solar masses). This is very likely to be due to the prolonged accretion episode that is thought to be required to form a MSP. The likely existence of massive MSPs makes them a powerful probe for understanding the behavior of matter at densities larger than that of the atomic nucleus; in particular, the precise measurement of the mass of PSR J1903+0327 ($1.67 +/- 0.01 solar masses) excludes several "soft" equations of state for dense matter.

  11. Binaries and distances

    Science.gov (United States)

    Pourbaix, D.; Arenou, F.; Halbwachs, J.-L.; Siopis, C.

    2013-02-01

    Gaia's five-year observation baseline might naively lead to the expectation that it will be possible to fit the parallax of any sufficiently nearby object with the default five-parameter model (position at a reference epoch, parallax and proper motion). However, simulated Gaia observations of a `model Universe' composed of nearly 107 objects, 50% of which turn out to be multiple stars, show that the single-star hypothesis can severely affect parallax estimation and that more sophisticated models must be adopted. In principle, screening these spurious single-star solutions is rather straightforward, for example by evaluating the quality of the fits. However, the simulated Gaia observations also reveal that some seemingly acceptable single-star solutions can nonetheless lead to erroneous distances. These solutions turn out to be binaries with an orbital period close to one year. Without auxiliary (e.g., spectroscopic) data, they will remain unnoticed.

  12. Binary black hole spectroscopy

    International Nuclear Information System (INIS)

    We study parameter estimation with post-Newtonian (PN) gravitational waveforms for the quasi-circular, adiabatic inspiral of spinning binary compact objects. In particular, the performance of amplitude-corrected waveforms is compared with that of the more commonly used restricted waveforms, in Advanced LIGO and EGO. With restricted waveforms, the properties of the source can only be extracted from the phasing. In the case of amplitude-corrected waveforms, the spectrum encodes a wealth of additional information, which leads to dramatic improvements in parameter estimation. At distances of ∼100 Mpc, the full PN waveforms allow for high-accuracy parameter extraction for total mass up to several hundred solar masses, while with the restricted ones the errors are steep functions of mass, and accurate parameter estimation is only possible for relatively light stellar mass binaries. At the low-mass end, the inclusion of amplitude corrections reduces the error on the time of coalescence by an order of magnitude in Advanced LIGO and a factor of 5 in EGO compared to the restricted waveforms; at higher masses these differences are much larger. The individual component masses, which are very poorly determined with restricted waveforms, become measurable with high accuracy if amplitude-corrected waveforms are used, with errors as low as a few per cent in Advanced LIGO and a few tenths of a per cent in EGO. The usual spin-orbit parameter β is also poorly determined with restricted waveforms (except for low-mass systems in EGO), but the full waveforms give errors that are small compared to the largest possible value consistent with the Kerr bound. This suggests a way of finding out if one or both of the component objects violate this bound. On the other hand, we find that the spin-spin parameter σ remains poorly determined even when the full waveform is used. Generally, all errors have but a weak dependence on the magnitudes and orientations of the spins. We also briefly

  13. Strong Lensing by Binary Galaxies

    CERN Document Server

    Shin, E M

    2008-01-01

    We study the problem of gravitational lensing by binary galaxies, idealized as two isothermal spheres. In a wide binary, each galaxy possesses individual tangential, nearly astroidal, caustics and roundish radial caustics. As the separation of the binary is made smaller, the caustics undergo a sequence of metamorphoses. The first metamorphosis occurs when the tangential caustics merge to form a single six-cusped caustic, lying interior to the radial caustics. At still smaller separations, the six-cusped caustic undergoes the second metamorphosis and splits into a four-cusped caustic and two three-cusped caustics, which shrink to zero size (an elliptic umbilic catastrophe) before they enlarge again and move away from the origin perpendicular to the binary axis. Finally, a third metamorphosis occurs as the three-cusp caustics join the radial caustics, leaving an inner distorted astroid caustic enclosed by two outer caustics. The maximum number of images possible is 7. Classifying the multiple imaging according ...

  14. Magnetic braking in ultracompact binaries

    CERN Document Server

    Farmer, Alison

    2010-01-01

    Angular momentum loss in ultracompact binaries, such as the AM Canum Venaticorum stars, is usually assumed to be due entirely to gravitational radiation. Motivated by the outflows observed in ultracompact binaries, we investigate whether magnetically coupled winds could in fact lead to substantial additional angular momentum losses. We remark that the scaling relations often invoked for the relative importance of gravitational and magnetic braking do not apply, and instead use simple non-empirical expressions for the braking rates. In order to remove significant angular momentum, the wind must be tied to field lines anchored in one of the binary's component stars; uncertainties remain as to the driving mechanism for such a wind. In the case of white dwarf accretors, we find that magnetic braking can potentially remove angular momentum on comparable or even shorter timescales than gravitational waves over a large range in orbital period. We present such a solution for the 17-minute binary AM CVn itself which a...

  15. Discs in misaligned binary systems

    CERN Document Server

    Rawiraswattana, Krisada; Goodwin, Simon P

    2016-01-01

    We perform SPH simulations to study precession and changes in alignment between the circumprimary disc and the binary orbit in misaligned binary systems. We find that the precession process can be described by the rigid-disc approximation, where the disc is considered as a rigid body interacting with the binary companion only gravitationally. Precession also causes change in alignment between the rotational axis of the disc and the spin axis of the primary star. This type of alignment is of great important for explaining the origin of spin-orbit misaligned planetary systems. However, we find that the rigid-disc approximation fails to describe changes in alignment between the disc and the binary orbit. This is because the alignment process is a consequence of interactions that involve the fluidity of the disc, such as the tidal interaction and the encounter interaction. Furthermore, simulation results show that there are not only alignment processes, which bring the components towards alignment, but also anti-...

  16. Cryptography with DNA binary strands.

    Science.gov (United States)

    Leier, A; Richter, C; Banzhaf, W; Rauhe, H

    2000-06-01

    Biotechnological methods can be used for cryptography. Here two different cryptographic approaches based on DNA binary strands are shown. The first approach shows how DNA binary strands can be used for steganography, a technique of encryption by information hiding, to provide rapid encryption and decryption. It is shown that DNA steganography based on DNA binary strands is secure under the assumption that an interceptor has the same technological capabilities as sender and receiver of encrypted messages. The second approach shown here is based on steganography and a method of graphical subtraction of binary gel-images. It can be used to constitute a molecular checksum and can be combined with the first approach to support encryption. DNA cryptography might become of practical relevance in the context of labelling organic and inorganic materials with DNA 'barcodes'. PMID:10963862

  17. AN IMPROVED DESIGN OF REVERSIBLE BINARY TO BINARY CODED DECIMAL CONVERTER FOR BINARY CODED DECIMAL MULTIPLICATION

    Directory of Open Access Journals (Sweden)

    Praveena Murugesan

    2014-01-01

    Full Text Available Reversible logic gates under ideal conditions produce zero power dissipation. This factor highlights the usage of these gates in optical computing, low power CMOS design, quantum optics and quantum computing. The growth of decimal arithmetic in various applications as stressed the need to propose the study on reversible binary to BCD converter which plays a greater role in decimal multiplication for providing faster results. The different parameters such as gate count,garbage output and constant input are more optimized in the proposed fixed bit binary to binary coded decimal converter than the existing design.

  18. Transient Black Hole Binaries

    CERN Document Server

    Belloni, T M

    2016-01-01

    The last two decades have seen a great improvement in our understand- ing of the complex phenomenology observed in transient black-hole binary systems, especially thanks to the activity of the Rossi X-Ray Timing Explorer satellite, com- plemented by observations from many other X-ray observatories and ground-based radio, optical and infrared facilities. Accretion alone cannot describe accurately the intricate behavior associated with black-hole transients and it is now clear that the role played by different kinds of (often massive) outflows seen at different phases of the outburst evolution of these systems is as fundamental as the one played by the accretion process itself. The spectral-timing states originally identified in the X-rays and fundamentally based on the observed effect of accretion, have acquired new importance as they now allow to describe within a coherent picture the phenomenology observed at other wave- length, where the effects of ejection processes are most evident. With a particular focu...

  19. Binary nucleation beyond capillarity approximation

    OpenAIRE

    Kalikmanov, V.I.

    2010-01-01

    Large discrepancies between binary classical nucleation theory (BCNT) and experiments result from adsorption effects and inability of BCNT, based on the phenomenological capillarity approximation, to treat small clusters. We propose a model aimed at eliminating both of these deficiencies. Adsorption is taken into account within Gibbsian approximation. Binary clusters are treated by means of statistical-mechanical considerations: tracing out the molecular degrees of freedom of the more volatil...

  20. Clostridium difficile binary toxin CDT

    OpenAIRE

    Gerding, Dale N.; Johnson, Stuart; Rupnik, Maja; Aktories, Klaus

    2013-01-01

    Binary toxin (CDT) is frequently observed in Clostridium difficile strains associated with increased severity of C. difficile infection (CDI). CDT belongs to the family of binary ADP-ribosylating toxins consisting of two separate toxin components: CDTa, the enzymatic ADP-ribosyltransferase which modifies actin, and CDTb which binds to host cells and translocates CDTa into the cytosol. CDTb is activated by serine proteases and binds to lipolysis stimulated lipoprotein receptor. ADP-ribosylatio...

  1. Coalescence of Binary Neutron Stars

    OpenAIRE

    Oohara, Ken-ichi; Namamura, Takashi

    1996-01-01

    The most important sources for laser-interferometric gravitational-wave detectors like LIGO or VIRGO are catastrophic events such as coalescence of a neutron-star binary. The final phase, or the last three milliseconds, of coalescence is considered. We describe results of numerical simulations of coalescing binary neutron stars using Newtonian and post-Newtonian hydrodynamics code and then discuss recent development of our 3D GR code.

  2. Binary Encodings of Non-binary Constraint Satisfaction Problems: Algorithms and Experimental Results

    CERN Document Server

    Samaras, N; 10.1613/jair.1776

    2011-01-01

    A non-binary Constraint Satisfaction Problem (CSP) can be solved directly using extended versions of binary techniques. Alternatively, the non-binary problem can be translated into an equivalent binary one. In this case, it is generally accepted that the translated problem can be solved by applying well-established techniques for binary CSPs. In this paper we evaluate the applicability of the latter approach. We demonstrate that the use of standard techniques for binary CSPs in the encodings of non-binary problems is problematic and results in models that are very rarely competitive with the non-binary representation. To overcome this, we propose specialized arc consistency and search algorithms for binary encodings, and we evaluate them theoretically and empirically. We consider three binary representations; the hidden variable encoding, the dual encoding, and the double encoding. Theoretical and empirical results show that, for certain classes of non-binary constraints, binary encodings are a competitive op...

  3. Transfection with the ribozyme targeting HPVE6 mRNA results in growth inhibition of E6-expressing cervical carcinoma cells

    Institute of Scientific and Technical Information of China (English)

    郑燕芳; 张积仁

    2003-01-01

    Objective: To acquire a ribozyme against the E6 gene of human papillomaviruses type 16 (HPV16E6) and investigate its effects on the phenotypes and gene expression of cervical cancer cell line. Methods: Anti-HPV16E6 ribozyme (HRz) was designed by computer programs and its activity identified by cleavage experiment in vitro before its transfection via lipofectin into CaSKi cells with the empty eucaryotic expression plasmid transfection of the cells also performed, the resultant cells designated as CaSKi-R, CaSKi-P respectively. The morphology and the soft agar forming ability were studied in CaSKi cells and the transfected cells, and the expression of E6, proliferating cell nuclear antigen (PCNA) and C-erbB-2 genes assayed by flow cytometry. The tumorgenicity of each cell line was evaluated in nude mice receiving inoculations of CaSKi, CaSKi-R and CaSKi-P cells separately, while in one group, both CaSKi and CaSKi-R cells were inoculated on different sides of the mice. Results: HRz was able to cleave HPV16E6 mRNA in a site-specific manner and could be expressed stably in transfected CaSKi cells. Northern blot analysis showed that E6 mRNA was less in CaSKi-R than in CaSKi cells, and no significant difference in the morphology and growth rate was observed between CaSKi and CaSKi-P cells, but the growth rate CaSKi-R was lowered. The colony-forming rate of CaSKi-P in soft agar was similar to that of CaSKi cells, while that of CaSKi-R was decreased. Flow cytometry showed that anti-HPV16E6 ribozyme reduced the expression of E6, PCNA and C-erbB-2 genes in CaSKi-R cells, but not in CaSKi-P cells. The tumorgenicity of CaSKi-R in nude mice was decreased compared with CaSKi cells. Conclusion: HRz can partially reverse the malignant phenotype of CaSKi cells, possibly due to decreased E6 gene expression, and the consequent decrease of PCNA and C-erbB-2 gene expressions.

  4. Binaries and Globular Cluster Dynamics

    CERN Document Server

    Rasio, F A; Joshi, K J; Rasio, Frederic A.; Fregeau, John M.; Joshi, Kriten J.

    2001-01-01

    We summarize the results of recent theoretical work on the dynamical evolution of globular clusters containing primordial binaries. Even a very small initial binary fraction (e.g., 10%) can play a key role in supporting a cluster against gravothermal collapse for many relaxation times. Inelastic encounters between binaries and single stars or other binaries provide a very significant energy source for the cluster. These dynamical interactions also lead to the production of large numbers of exotic systems such as ultracompact X-ray binaries, recycled radio pulsars, double degenerate systems, and blue stragglers. Our work is based on a new parallel supercomputer code implementing Henon's Monte Carlo method for simulating the dynamical evolution of dense stellar systems in the Fokker-Planck approximation. This new code allows us to calculate very accurately the evolution of a cluster containing a realistic number of stars (N ~ 10^5 - 10^6) in typically a few hours to a few days of computing time. The discrete, s...

  5. Exoplanets Bouncing Between Binary Stars

    CERN Document Server

    Moeckel, Nickolas

    2012-01-01

    Exoplanetary systems are found not only among single stars, but also binaries of widely varying parameters. Binaries with separations of 100--1000 au are prevalent in the Solar neighborhood; at these separations planet formation around a binary member may largely proceed as if around a single star. During the early dynamical evolution of a planetary system, planet--planet scattering can eject planets from a star's grasp. In a binary, the motion of a planet ejected from one star has effectively entered a restricted three-body system consisting of itself and the two stars, and the equations of motion of the three body problem will apply as long as the ejected planet remains far from the remaining planets. Depending on its energy, escape from the binary as a whole may be impossible or delayed until the three-body approximation breaks down, and further close interactions with its planetary siblings boost its energy when it passes close to its parent star. Until then this planet may be able to transition from the ...

  6. Using Binary Code Instrumentation in Computer Security

    Directory of Open Access Journals (Sweden)

    Marius POPA

    2013-01-01

    Full Text Available The paper approaches the low-level details of the code generated by compilers whose format permits outside actions. Binary code modifications are manually done when the internal format is known and understood, or automatically by certain tools developed to process the binary code. The binary code instrumentation goals may be various from security increasing and bug fixing to development of malicious software. The paper highlights the binary code instrumentation techniques by code injection to increase the security and reliability of a software application. Also, the paper offers examples for binary code formats understanding and how the binary code injection may be applied.

  7. The structure of contact binaries

    CERN Document Server

    Kaehler, H

    2003-01-01

    In radiative layers of rotating stars the luminosity carried by circulation currents through a surface of constant entropy (circulation luminosity) is shown to be positive. The corresponding decrease in the temperature gradient is important in the secondary of contact binaries. This result removes the deadlock in the theory of contact binaries. The resulting treatment of contact binaries is investigated, assuming thermal equilibrium. If the circulation luminosity is adjusted to give a prescribed temperature difference between the components, details turn out to be unimportant. The temperature difference is bound to be positive. The fractional extent of radiative regions is larger in the secondary than in the primary. In the course of evolution the period increases and the mass ratio decreases. A survey of unevolved and evolved contact configurations is presented. Observational tests are passed. In stable systems the degree of contact is small. Stable systems in the period-colour diagram, unevolved and evolved...

  8. Practical Binary Adaptive Block Coder

    CERN Document Server

    Reznik, Yuriy A

    2007-01-01

    This paper describes design of a low-complexity algorithm for adaptive encoding/ decoding of binary sequences produced by memoryless sources. The algorithm implements universal block codes constructed for a set of contexts identified by the numbers of non-zero bits in previous bits in a sequence. We derive a precise formula for asymptotic redundancy of such codes, which refines previous well-known estimate by Krichevsky and Trofimov, and provide experimental verification of this result. In our experimental study we also compare our implementation with existing binary adaptive encoders, such as JBIG's Q-coder, and MPEG AVC (ITU-T H.264)'s CABAC algorithms.

  9. Coalescing binaries and Doppler experiments

    OpenAIRE

    Vecchio, A.; Bertotti, B.; Iess, L.

    1997-01-01

    We discuss the sensitivity of the CASSINI experiments to gravitational waves emitted by the in-spiral of compact binaries. We show that the maximum distance reachable by the instrument is $\\sim 100$ Mpc. In particular, CASSINI can detect massive black hole binaries with chirp mass $\\simgt 10^6 \\Ms$ in the Virgo Cluster with signal-to-noise ratio between 5 and 30 and possible compact objects of mass $\\simgt 30 \\Ms$ orbiting the massive black hole that our Galactic Centre is likely to harbour.

  10. Rectangular Decomposition of Binary Images

    Czech Academy of Sciences Publication Activity Database

    Suk, Tomáš; Höschl, Cyril; Flusser, Jan

    Berlin : Springer, 2012 - (Blanc-Talon, J.; Popescu, D.; Philips, W.; Scheunders, P.), s. 213-224 ISBN 978-3-642-33139-8. - (Lecture Notes in Computer Science. 7517). [Advanced Concepts for Intelligent Vision Systems (Acivs 2012). Brno (CZ), 04.09.2012-07.09.2012] R&D Projects: GA ČR GAP103/11/1552 Institutional support: RVO:67985556 Keywords : binary image decomposition * generalized delta-method * distance transformation * quadtree * bipartite graph * image compression * fast convolution Subject RIV: IN - Informatics, Computer Science http://library.utia.cas.cz/separaty/2012/ZOI/suk-rectangular decomposition of binary images.pdf

  11. Kepler Eclipsing Binaries with Stellar Companions

    CERN Document Server

    Gies, D R; Guo, Z; Lester, K V; Orosz, J A; Peters, G J

    2015-01-01

    Many short-period binary stars have distant orbiting companions that have played a role in driving the binary components into close separation. Indirect detection of a tertiary star is possible by measuring apparent changes in eclipse times of eclipsing binaries as the binary orbits the common center of mass. Here we present an analysis of the eclipse timings of 41 eclipsing binaries observed throughout the NASA Kepler mission of long duration and precise photometry. This subset of binaries is characterized by relatively deep and frequent eclipses of both stellar components. We present preliminary orbital elements for seven probable triple stars among this sample, and we discuss apparent period changes in seven additional eclipsing binaries that may be related to motion about a tertiary in a long period orbit. The results will be used in ongoing investigations of the spectra and light curves of these binaries for further evidence of the presence of third stars.

  12. Frame theory for binary vector spaces

    OpenAIRE

    Bodmann, Bernhard G.; Le, My; Reza, Letty; Tobin, Matthew; Tomforde, Mark

    2009-01-01

    We develop the theory of frames and Parseval frames for finite-dimensional vector spaces over the binary numbers. This includes characterizations which are similar to frames and Parseval frames for real or complex Hilbert spaces, and the discussion of conceptual differences caused by the lack of a proper inner product on binary vector spaces. We also define switching equivalence for binary frames, and list all equivalence classes of binary Parseval frames in lowest dimensions, excluding cases...

  13. Using Binary Code Instrumentation in Computer Security

    OpenAIRE

    Marius POPA; Sergiu Marin CAPISIZU

    2013-01-01

    The paper approaches the low-level details of the code generated by compilers whose format permits outside actions. Binary code modifications are manually done when the internal format is known and understood, or automatically by certain tools developed to process the binary code. The binary code instrumentation goals may be various from security increasing and bug fixing to development of malicious software. The paper highlights the binary code instrumentation techniques by code injection to...

  14. Discs in misaligned binary systems

    Science.gov (United States)

    Rawiraswattana, Krisada; Hubber, David A.; Goodwin, Simon P.

    2016-08-01

    We perform SPH simulations to study precession and changes in alignment between the circumprimary disc and the binary orbit in misaligned binary systems. We find that the precession process can be described by the rigid-disc approximation, where the disc is considered as a rigid body interacting with the binary companion only gravitationally. Precession also causes change in alignment between the rotational axis of the disc and the spin axis of the primary star. This type of alignment is of great important for explaining the origin of spin-orbit misaligned planetary systems. However, we find that the rigid-disc approximation fails to describe changes in alignment between the disc and the binary orbit. This is because the alignment process is a consequence of interactions that involve the fluidity of the disc, such as the tidal interaction and the encounter interaction. Furthermore, simulation results show that there are not only alignment processes, which bring the components towards alignment, but also anti-alignment processes, which tend to misalign the components. The alignment process dominates in systems with misalignment angle near 90°, while the anti-alignment process dominates in systems with the misalignment angle near 0° or 180°. This means that highly misaligned systems will become more aligned but slightly misaligned systems will become more misaligned.

  15. A Galactic Binary Detection Pipeline

    Science.gov (United States)

    Littenberg, Tyson B.

    2011-01-01

    The Galaxy is suspected to contain hundreds of millions of binary white dwarf systems, a large fraction of which will have sufficiently small orbital period to emit gravitational radiation in band for space-based gravitational wave detectors such as the Laser Interferometer Space Antenna (LISA). LISA's main science goal is the detection of cosmological events (supermassive black hole mergers, etc.) however the gravitational signal from the galaxy will be the dominant contribution to the data - including instrumental noise over approximately two decades in frequency. The catalogue of detectable binary systems will serve as an unparalleled means of studying the Galaxy. Furthermore, to maximize the scientific return from the mission, the data must be "cleansed" of the galactic foreground. We will present an algorithm that can accurately resolve and subtract 2:: 10000 of these sources from simulated data supplied by the Mock LISA Data Challenge Task Force. Using the time evolution of the gravitational wave frequency, we will reconstruct the position of the recovered binaries and show how LISA will sample the entire compact binary population in the Galaxy.

  16. CFD Simulations of Binary Nucleation

    Czech Academy of Sciences Publication Activity Database

    Herrmann, E.; Brus, David; Hyvärinen, A-P.; Kulmala, M.

    Helsinki : -, 2010, P3U16. ISBN N. [International Aerosol Conference IAC 2010. Helsinki (FI), 29.08.2010-03.09.2010] Grant ostatní: FCR(FI) 1118615 Institutional research plan: CEZ:AV0Z40720504 Keywords : nucleation * binary * parameterization Subject RIV: CF - Physical ; Theoretical Chemistry www.iac2010.fi

  17. Eccentricity distribution of wide binaries

    CERN Document Server

    Tokovinin, Andrei

    2015-01-01

    A sample of 477 solar-type binaries within 67pc with projected separations larger than 50AU is studied by a new statistical method. Speed and direction of the relative motion are determined from the short observed arcs or known orbits, and their joint distribution is compared to the numerical simulations. By inverting the observed distribution with the help of simulations, we find that average eccentricity of wide binaries is 0.59+-0.02 and the eccentricity distribution can be modeled as f(e) ~= 1.2 e + 0.4. However, wide binaries containing inner subsystems, i.e. triple or higher-order multiples, have significantly smaller eccentricities with the average e = 0.52+-0.05 and the peak at e ~ 0.5. We find that the catalog of visual orbits is strongly biased against large eccentricities. A marginal evidence of eccentricity increasing with separation (or period) is found for this sample. Comparison with spectroscopic binaries proves the reality of the controversial period-eccentricity relation. The average eccentr...

  18. A Redundant Binary Algorithm for RSA

    Institute of Scientific and Technical Information of China (English)

    施荣华

    1996-01-01

    The normal form and modified normal form for binary redundant representation are defined.A redundant binary algorithm to compute modular exponentiation for very large integers is proposed.It is shown that the proposed algorithm requires the minimum number of basic operations(modular multiplications)among all possible binary redundant representations.

  19. Competitive learning for binary valued data

    OpenAIRE

    Leisch, Friedrich; Weingessel, Andreas; Dimitriadou, Evgenia

    1998-01-01

    We propose a new approach for using online competitive learning on binary data. The usual Euclidean distance is replaced by binary distance measures, which take possible asymmetries of binary data into account and therefore provide a "different point of view" for looking at the data. The method is demonstrated on two artificial examples and applied on tourist marketing research data. (author's abstract)

  20. Permutation Entropy for Random Binary Sequences

    Directory of Open Access Journals (Sweden)

    Lingfeng Liu

    2015-12-01

    Full Text Available In this paper, we generalize the permutation entropy (PE measure to binary sequences, which is based on Shannon’s entropy, and theoretically analyze this measure for random binary sequences. We deduce the theoretical value of PE for random binary sequences, which can be used to measure the randomness of binary sequences. We also reveal the relationship between this PE measure with other randomness measures, such as Shannon’s entropy and Lempel–Ziv complexity. The results show that PE is consistent with these two measures. Furthermore, we use PE as one of the randomness measures to evaluate the randomness of chaotic binary sequences.

  1. Remnants of compact binary mergers

    CERN Document Server

    Domainko, W

    2006-01-01

    We investigate the long-term evolution and observability of remnants originating from the merger of compact binary systems and discuss the differences to supernova remnants. Compact binary mergers expel much smaller amounts of mass at much higher velocities, as compared to supernovae, which will affect the dynamical evolution of their remnants. The ejecta of mergers consist of very neutron rich nuclei. Some of these neutron rich nuclei will produce observational signatures in form of gamma ray lines during their decay. The composition of the ejecta might even give interesting constraints about the internal structure of the neutron star. We further discuss the possibility that merger remnants appear as recently discovered 'dark accelerators' which are extended TeV sources which lack emission in other bands.

  2. Event Rates for Binary Inspiral

    CERN Document Server

    Kalogera, V

    2001-01-01

    Double compact objects (neutron stars and black holes) found in binaries with small orbital separations are known to spiral in and are expected to coalesce eventually because of the emission of gravitational waves. Such inspiral and merger events are thought to be primary sources for ground based gravitational-wave interferometric detectors (such as LIGO). Here, we present a brief review of estimates of coalescence rates and we examine the origin and relative importance of uncertainties associated with the rate estimates. For the case of double neutron star systems, we compare the most recent rate estimates to upper limits derived in a number of different ways. We also discuss the implications of the formation of close binaries with two non-recycled pulsars.

  3. Modified binary particle swam optimization

    Institute of Scientific and Technical Information of China (English)

    Sangwook Lee; Sangmoon Soak; Sanghoun Oh; Witold Pedrycz; Moongu Jeon

    2008-01-01

    This paper presents a modified binary particle swarm optimization(BPSO)which adopts concepts of the genotype-phenotype rep-resentation and the mutation operator of genetic algorithms.Its main feature is that the BPSO can be treated as a continuous PSO.The proposed BPSO algorithm is tested on various benchmark functions,and its performance is compared with that of the original BPSO.Experimental results show that the modified BPSO outperforms the original BPSO algorithm.

  4. Tides in asynchronous binary systems

    OpenAIRE

    Toledano, Oswaldo; Moreno, Edmundo; Koenigsberger, Gloria; Detmers, R.; Langer, Norbert

    2006-01-01

    Stellar oscillations are excited in non-synchronously rotating stars in binary systems due to the tidal forces. Tangential components of the tides can drive a shear flow which behaves as a differentially forced rotating structure in a stratified outer medium. In this paper we show that our single-layer approximation for the calculation of the forced oscillations yields results that are consistent with the predictions for the synchronization timescales in circular orbits. In addition, calibrat...

  5. Galaxy Rotation and Rapid Supermassive Binary Coalescence

    Science.gov (United States)

    Holley-Bockelmann, Kelly; Khan, Fazeel Mahmood

    2015-09-01

    Galaxy mergers usher the supermassive black hole (SMBH) in each galaxy to the center of the potential, where they form an SMBH binary. The binary orbit shrinks by ejecting stars via three-body scattering, but ample work has shown that in spherical galaxy models, the binary separation stalls after ejecting all the stars in its loss cone—this is the well-known final parsec problem. However, it has been shown that SMBH binaries in non-spherical galactic nuclei harden at a nearly constant rate until reaching the gravitational wave regime. Here we use a suite of direct N-body simulations to follow SMBH binary evolution in both corotating and counterrotating flattened galaxy models. For N > 500 K, we find that the evolution of the SMBH binary is convergent and is independent of the particle number. Rotation in general increases the hardening rate of SMBH binaries even more effectively than galaxy geometry alone. SMBH binary hardening rates are similar for co- and counterrotating galaxies. In the corotating case, the center of mass of the SMBH binary settles into an orbit that is in corotation resonance with the background rotating model, and the coalescence time is roughly a few 100 Myr faster than a non-rotating flattened model. We find that counterrotation drives SMBHs to coalesce on a nearly radial orbit promptly after forming a hard binary. We discuss the implications for gravitational wave astronomy, hypervelocity star production, and the effect on the structure of the host galaxy.

  6. Visual Binaries in the Orion Nebula Cluster

    CERN Document Server

    Reipurth, Bo; Connelley, Michael S; Bally, John

    2007-01-01

    We have carried out a major survey for visual binaries towards the Orion Nebula Cluster using HST images obtained with an H-alpha filter. Among 781 likely ONC members more than 60" from theta-1 Ori C, we find 78 multiple systems (75 binaries and 3 triples), of which 55 are new discoveries, in the range from 0.1" to 1.5". About 9 binaries are likely line-of-sight associations. We find a binary fraction of 8.8%+-1.1% within the limited separation range from 67.5 to 675 AU. The field binary fraction in the same range is a factor 1.5 higher. Within the range 150 AU to 675 AU we find that T Tauri associations have a factor 2.2 more binaries than the ONC. The binary separation distribution function of the ONC shows unusual structure, with a sudden steep decrease in the number of binaries as the separation increases beyond 0.5", corresponding to 225 AU. We have measured the ratio of binaries wider than 0.5" to binaries closer than 0.5" as a function of distance from the Trapezium, and find that this ratio is signifi...

  7. Evolution of binary stars in multiple-population globular clusters - II. Compact binaries

    Science.gov (United States)

    Hong, Jongsuk; Vesperini, Enrico; Sollima, Antonio; McMillan, Stephen L. W.; D'Antona, Franca; D'Ercole, Annibale

    2016-04-01

    We present the results of a survey of N-body simulations aimed at exploring the evolution of compact binaries in multiple-population globular clusters. We show that as a consequence of the initial differences in the structural properties of the first-generation (FG) and the second-generation (SG) populations and the effects of dynamical processes on binary stars, the SG binary fraction decreases more rapidly than that of the FG population. The difference between the FG and SG binary fraction is qualitatively similar to but quantitatively smaller than that found for wider binaries in our previous investigations. The evolution of the radial variation of the binary fraction is driven by the interplay between binary segregation, ionization and ejection. Ionization and ejection counteract in part the effects of mass segregation but for compact binaries the effects of segregation dominate and the inner binary fraction increases during the cluster evolution. We explore the variation of the difference between the FG and the SG binary fraction with the distance from the cluster centre and its dependence on the binary binding energy and cluster structural parameters. The difference between the binary fraction in the FG and the SG populations found in our simulations is consistent with the results of observational studies finding a smaller binary fraction in the SG population.

  8. Orbital eccentricities in primordial black holes binaries

    OpenAIRE

    Cholis, Ilias; Kovetz, Ely D.; Ali-Haïmoud, Yacine; Bird, Simeon; Kamionkowski, Marc; Muñoz, Julian B.; Raccanelli, Alvise

    2016-01-01

    It was recently suggested that the merger of $\\sim30\\,M_\\odot$ primordial black holes (PBHs) may provide a significant number of events in gravitational-wave observatories over the next decade, if they make up an appreciable fraction of the dark matter. Here we show that measurement of the eccentricities of the inspiralling binary black holes can be used to distinguish these binaries from those produced by more traditional astrophysical mechanisms. These PBH binaries are formed on highly ecce...

  9. Asteroid Systems: Binaries, Triples, and Pairs

    CERN Document Server

    Margot, Jean-Luc; Taylor, Patrick; Carry, Benoît; Jacobson, Seth

    2015-01-01

    In the past decade, the number of known binary near-Earth asteroids has more than quadrupled and the number of known large main belt asteroids with satellites has doubled. Half a dozen triple asteroids have been discovered, and the previously unrecognized populations of asteroid pairs and small main belt binaries have been identified. The current observational evidence confirms that small (20 km) binaries with small satellites are most likely created during large collisions.

  10. Relativistic Gravity and Binary Radio Pulsars

    OpenAIRE

    Kaspi, V. M.

    1999-01-01

    Following a summary of the basic principles of pulsar timing, we present a review of recent results from timing observations of relativistic binary pulsars. In particular, we summarize the status of timing observations of the much celebrated original binary pulsar PSR B1913+16, draw attention to the recent confirmation of strong evidence for geodetic precession in this system, review the recent measurement of multiple post-Keplerian binary parameters for PSR B1534+12, and describe the Parkes ...

  11. Microlensing Signature of Binary Black Holes

    Science.gov (United States)

    Schnittman, Jeremy; Sahu, Kailash; Littenberg, Tyson

    2012-01-01

    We calculate the light curves of galactic bulge stars magnified via microlensing by stellar-mass binary black holes along the line-of-sight. We show the sensitivity to measuring various lens parameters for a range of survey cadences and photometric precision. Using public data from the OGLE collaboration, we identify two candidates for massive binary systems, and discuss implications for theories of star formation and binary evolution.

  12. Detection of unresolved binaries with multicolor photometry

    CERN Document Server

    Chulkov, D; Malkov, O; Sichevskij, S; Krussanova, N; Mironov, A; Zakharov, A; Kniazev, A

    2016-01-01

    The principal goal of this paper is to specify conditions of detection of unresolved binaries by multicolor photometry. We have developed a method for estimating the critical distance at which an unresolved binary of given mass and age can be detected. The method is applied to the photometric system of the planned Lyra-B spaceborne experiment. We have shown that some types of unresolved binary stars can be discovered and distinguished from single stars solely by means of photometric observations.

  13. Relativistic Gravity and Binary Radio Pulsars

    CERN Document Server

    Kaspi, V M

    1999-01-01

    Following a summary of the basic principles of pulsar timing, we present a review of recent results from timing observations of relativistic binary pulsars. In particular, we summarize the status of timing observations of the much celebrated original binary pulsar PSR B1913+16, draw attention to the recent confirmation of strong evidence for geodetic precession in this system, review the recent measurement of multiple post-Keplerian binary parameters for PSR B1534+12, and describe the Parkes Multibeam survey, a major survey of the Galactic Plane which promises to discover new relativistic binary pulsar systems.

  14. Speech perception of noise with binary gains

    DEFF Research Database (Denmark)

    Wang, DeLiang; Kjems, Ulrik; Pedersen, Michael Syskind;

    2008-01-01

    For a given mixture of speech and noise, an ideal binary time-frequency mask is constructed by comparing speech energy and noise energy within local time-frequency units. It is observed that listeners achieve nearly perfect speech recognition from gated noise with binary gains prescribed by the i...... by the ideal binary mask. Only 16 filter channels and a frame rate of 100 Hz are sufficient for high intelligibility. The results show that, despite a dramatic reduction of speech information, a pattern of binary gains provides an adequate basis for speech perception....

  15. The equations for binary density and the binary stream of particles of electro conducting magnetic liquids

    International Nuclear Information System (INIS)

    In work on the basis of a method of the kinetic equations it is output the differential equations for binary density and a binary stream of particles of electro conducting magnetic liquids. These equations are the nonuniform equations of parabolic type. The solution of these equations completely feature existential behaviour of binary density and a binary stream of particles of electro conducting magnetic liquids, i.e. process of a structural relaxation. (author)

  16. Massive Stars in Interactive Binaries

    Science.gov (United States)

    St.-Louis, Nicole; Moffat, Anthony F. J.

    Massive stars start their lives above a mass of ~8 time solar, finally exploding after a few million years as core-collapse or pair-production supernovae. Above ~15 solar masses, they also spend most of their lives driving especially strong, hot winds due to their extreme luminosities. All of these aspects dominate the ecology of the Universe, from element enrichment to stirring up and ionizing the interstellar medium. But when they occur in close pairs or groups separated by less than a parsec, the interaction of massive stars can lead to various exotic phenomena which would not be seen if there were no binaries. These depend on the actual separation, and going from wie to close including colliding winds (with non-thermal radio emission and Wolf-Rayet dust spirals), cluster dynamics, X-ray binaries, Roche-lobe overflow (with inverse mass-ratios and rapid spin up), collisions, merging, rejuventation and massive blue stragglers, black-hole formation, runaways and gamma-ray bursts. Also, one wonders whether the fact that a massive star is in a binary affects its parameters compared to its isolated equivalent. These proceedings deal with all of these phenomena, plus binary statistics and determination of general physical properties of massive stars, that would not be possible with their single cousins. The 77 articles published in these proceedings, all based on oral talks, vary from broad revies to the lates developments in the field. About a third of the time was spent in open discussion of all participants, both for ~5 minutes after each talk and 8 half-hour long general dialogues, all audio-recorded, transcribed and only moderately edited to yield a real flavour of the meeting. The candid information in these discussions is sometimes more revealing than the article(s) that preceded them and also provide entertaining reading. The book is suitable for researchers and graduate students interested in stellar astrophysics and in various physical processes involved when

  17. Young and Waltzing Binary Stars

    Science.gov (United States)

    2001-10-01

    ADONIS Observes Low-mass Eclipsing System in Orion Summary A series of very detailed images of a binary system of two young stars have been combined into a movie . In merely 3 days, the stars swing around each other. As seen from the earth, they pass in front of each other twice during a full revolution, producing eclipses during which their combined brightness diminishes . A careful analysis of the orbital motions has now made it possible to deduce the masses of the two dancing stars . Both turn out to be about as heavy as our Sun. But while the Sun is about 4500 million years old, these two stars are still in their infancy. They are located some 1500 light-years away in the Orion star-forming region and they probably formed just 10 million years ago . This is the first time such an accurate determination of the stellar masses could be achieved for a young binary system of low-mass stars . The new result provides an important piece of information for our current understanding of how young stars evolve. The observations were obtained by a team of astronomers from Italy and ESO [1] using the ADaptive Optics Near Infrared System (ADONIS) on the 3.6-m telescope at the ESO La Silla Observatory. PR Photo 29a/01 : The RXJ 0529.4+0041 system before primary eclipse PR Photo 29b/01 : The RXJ 0529.4+0041 system at mid-primary eclipse PR Photo 29c/01 : The RXJ 0529.4+0041 system after primary eclipse PR Photo 29d/01 : The RXJ 0529.4+0041 system before secondary eclipse PR Photo 29e/01 : The RXJ 0529.4+0041 system at mid-secondary eclipse PR Photo 29f/01 : The RXJ 0529.4+0041 system after secondary eclipse PR Video Clip 06/01 : Video of the RXJ 0529.4+0041 system Binary stars and stellar masses Since some time, astronomers have noted that most stars seem to form in binary or multiple systems. This is quite fortunate, as the study of binary stars is the only way in which it is possible to measure directly one of the most fundamental quantities of a star, its mass. The mass of a

  18. The structures of binary compounds

    CERN Document Server

    Hafner, J; Jensen, WB; Majewski, JA; Mathis, K; Villars, P; Vogl, P; de Boer, FR

    1990-01-01

    - Up-to-date compilation of the experimental data on the structures of binary compounds by Villars and colleagues. - Coloured structure maps which order the compounds into their respective structural domains and present for the first time the local co-ordination polyhedra for the 150 most frequently occurring structure types, pedagogically very helpful and useful in the search for new materials with a required crystal structure. - Crystal co-ordination formulas: a flexible notation for the interpretation of solid-state structures by chemist Bill Jensen. - Recent important advances in unders

  19. R144 : a very massive binary likely ejected from R136 through a binary-binary encounter

    CERN Document Server

    Oh, Seungkyung; Banerjee, Sambaran

    2013-01-01

    R144 is a recently confirmed very massive, spectroscopic binary which appears isolated from the core of the massive young star cluster R136. The dynamical ejection hypothesis as an origin for its location is claimed improbable by Sana et al. due to its binary nature and high mass. We demonstrate here by means of direct N-body calculations that a very massive binary system can be readily dynamically ejected from a R136-like cluster, through a close encounter with a very massive system. One out of four N-body cluster models produces a dynamically ejected very massive binary system with a mass comparable to R144. The system has a system mass of $\\approx$ 355 Msun and is located at 36.8 pc from the centre of its parent cluster, moving away from the cluster with a velocity of 57 km/s at 2 Myr as a result of a binary-binary interaction. This implies that R144 could have been ejected from R136 through a strong encounter with an other massive binary or single star. In addition, we discuss all massive binaries and sin...

  20. Mercury and Selenium in Muscle and Target Organs of Scalloped Hammerhead Sharks Sphyrna lewini of the SE Gulf of California: Dietary Intake, Molar Ratios, Loads, and Human Health Risks.

    Science.gov (United States)

    Bergés-Tiznado, Magdalena E; Márquez-Farías, Fernando; Lara-Mendoza, Raúl E; Torres-Rojas, Yassir E; Galván-Magaña, Felipe; Bojórquez-Leyva, Humberto; Páez-Osuna, Federico

    2015-11-01

    Selenium and mercury were evaluated in muscle, liver, kidney, brain, and the stomach contents of juvenile scalloped hammerhead shark Sphyrna lewini. Se:Hg molar ratios were calculated. The average Hg levels in muscle ranged from 0.12 to 1.17 μg/g (wet weight); Hg was 1. Correlations were found for Hg in muscle with size, age, and weight, and also for Hg in liver with size, age, and weight. Hg in muscle was significantly positive correlated to Hg in brain as well as Hg in liver was correlated to Hg in kidney. The highest Hg in preys was for carangid fishes; scombrid and carangid fishes contributed with the highest Se levels. Results suggest that more than 98 % of the total Hg and 62 % of Se end up in muscle and might be affected by factors, such as geographical area, age, size, and feeding habits. The muscle of S. lewini should be consumed by people cautiously so as not to exceed the recommended intake per week. PMID:26369650

  1. Millisecond Pulsars in Close Binaries

    CERN Document Server

    Tauris, Thomas M

    2015-01-01

    In this Habilitationsschrift (Habilitation thesis) I present my research carried out over the last four years at the Argelander Institute for Astronomy (AIfA) and the Max Planck Institute for Radio Astronomy (MPIfR). The thesis summarizes my main findings and has been written to fulfill the requirements for the Habilitation qualification at the University of Bonn. Although my work is mainly focused on the topic of millisecond pulsars (MSPs), there is a fairly broad spread of research areas ranging from the formation of neutron stars (NSs) in various supernova (SN) events, to their evolution, for example, via accretion processes in binary and triple systems, and finally to their possible destruction in merger events. The thesis is organized in the following manner: A general introduction to neutron stars and millisecond pulsars is given in Chapter 1. A selection of key papers published in 2011-2014 are presented in Chapters 2-10, ordered within five main research areas (ultra-stripped SNe in close binaries, ma...

  2. Polarization in binary microlensing events

    International Nuclear Information System (INIS)

    The light received by source stars in microlensing events may be significantly polarized if both an efficient photon-scattering mechanism is active in the source stellar atmosphere and a differential magnification is therein induced by the lensing system. The best candidate events for observing polarization are highly magnified events with source stars belonging to the class of cool, giant stars in which the stellar light is polarized by photon scattering on dust grains contained in their envelopes. The presence in the stellar atmosphere of an internal cavity devoid of dust produces polarization profiles with a two peaks structure. Hence, the time interval between them gives an important observable quantity directly related to the size of the internal cavity and to the model parameters of the lens system. We show that, during a microlensing event, the expected polarization variability can solve an ambiguity that arises in some cases, related to the binary or planetary lensing interpretation of the perturbations observed near the maximum of the event light-curve. We consider a specific event case for which the parameter values corresponding to the two solutions are given. Then, assuming a polarization model for the source star, we compute the two expected polarization profiles. The position of the two peaks appearing in the polarization curves and the characteristic time interval between them allow us to distinguish between the binary and planetary lens solutions. (paper)

  3. Pulsating Components in Binary and Multiple Stellar Systems --- A Catalog of Oscillating Binaries

    OpenAIRE

    Zhou, A. -Y.

    2010-01-01

    We present an up-to-date catalog of pulsating binaries, i.e. the binary and multiple stellar systems containing pulsating components, along with a statistics on them. Compared to the earlier compilation by Soydugan et al.(2006a) of 25 delta Scuti-type `oscillating Algol-type eclipsing binaries' (oEA), the recent collection of 74 oEA by Liakos et al.(2012), and the collection of Cepheids in binaries by Szabados (2003a), the numbers and types of pulsating variables in binaries are now extended....

  4. The Evolution of Compact Binary Star Systems

    Directory of Open Access Journals (Sweden)

    Yungelson, Lev R.

    2006-12-01

    Full Text Available We review the formation and evolution of compact binary stars consisting of white dwarfs (WDs, neutron stars (NSs, and black holes (BHs. Binary NSs and BHs are thought to be the primary astrophysical sources of gravitational waves (GWs within the frequency band of ground-based detectors, while compact binaries of WDs are important sources of GWs at lower frequencies to be covered by space interferometers (LISA. Major uncertainties in the current understanding of properties of NSs and BHs most relevant to the GW studies are discussed, including the treatment of the natal kicks which compact stellar remnants acquire during the core collapse of massive stars and the common envelope phase of binary evolution. We discuss the coalescence rates of binary NSs and BHs and prospects for their detections, the formation and evolution of binary WDs and their observational manifestations. Special attention is given to AM CVn-stars -- compact binaries in which the Roche lobe is filled by another WD or a low-mass partially degenerate helium-star, as these stars are thought to be the best LISA verification binary GW sources.

  5. The Evolution of Compact Binary Star Systems

    Directory of Open Access Journals (Sweden)

    Konstantin A. Postnov

    2014-05-01

    Full Text Available We review the formation and evolution of compact binary stars consisting of white dwarfs (WDs, neutron stars (NSs, and black holes (BHs. Mergings of compact-star binaries are expected to be the most important sources for forthcoming gravitational-wave (GW astronomy. In the first part of the review, we discuss observational manifestations of close binaries with NS and/or BH components and their merger rate, crucial points in the formation and evolution of compact stars in binary systems, including the treatment of the natal kicks, which NSs and BHs acquire during the core collapse of massive stars and the common envelope phase of binary evolution, which are most relevant to the merging rates of NS-NS, NS-BH and BH-BH binaries. The second part of the review is devoted mainly to the formation and evolution of binary WDs and their observational manifestations, including their role as progenitors of cosmologically-important thermonuclear SN Ia. We also consider AM CVn-stars, which are thought to be the best verification binary GW sources for future low-frequency GW space interferometers.

  6. Eliciting Subjective Probabilities with Binary Lotteries

    DEFF Research Database (Denmark)

    Harrison, Glenn W.; Martínez-Correa, Jimmy; Swarthout, J. Todd

    We evaluate the binary lottery procedure for inducing risk neutral behavior in a subjective belief elicitation task. Harrison, Martínez-Correa and Swarthout [2013] found that the binary lottery procedure works robustly to induce risk neutrality when subjects are given one risk task defined over o...

  7. Pulsed Accretion onto Eccentric and Circular Binaries

    CERN Document Server

    Muñoz, Diego J

    2016-01-01

    We present numerical simulations of circumbinary accretion onto eccentric and circular binaries using the moving-mesh code AREPO. This is the first set of simulations to tackle the problem of binary accretion using a finite-volume scheme on a freely moving mesh, which allows for accurate measurements of accretion onto individual stars for arbitrary binary eccentricity. While accretion onto a circular binary shows bursts with period of ~5 times the binary period P_b,accretion onto an eccentric binary is predominantly modulated at the period ~1P_b. For an equal-mass circular binary, the accretion rates onto individual stars are quite similar to each other, following the same variable pattern in time. By contrast, for eccentric binaries, one of the stars can accrete at a rate 10-20 times larger than its companion. This "symmetry breaking" between the stars, however, alternates over timescales of order 200 P_b, and can be attributed to a slowly precessing, eccentric circumbinary disk. Over longer timescales, the ...

  8. ECCENTRIC EVOLUTION OF SUPERMASSIVE BLACK HOLE BINARIES

    International Nuclear Information System (INIS)

    In recent numerical simulations, it has been found that the eccentricity of supermassive black hole (SMBH)-intermediate black hole (IMBH) binaries grows toward unity through interactions with the stellar background. This increase of eccentricity reduces the merging timescale of the binary through the gravitational radiation to a value well below the Hubble time. It also gives a theoretical explanation of the existence of eccentric binaries such as that in OJ287. In self-consistent N-body simulations, this increase of eccentricity is always observed. On the other hand, the result of the scattering experiment between SMBH binaries and field stars indicated that the eccentricity dose not change significantly. This discrepancy leaves the high eccentricity of the SMBH binaries in N-body simulations unexplained. Here, we present a stellar-dynamical mechanism that drives the increase of the eccentricity of an SMBH binary with a large mass ratio. There are two key processes involved. The first one is the Kozai mechanism under a non-axisymmetric potential, which effectively randomizes the angular momenta of surrounding stars. The other is the selective ejection of stars with prograde orbits. Through these two mechanisms, field stars extract the orbital angular momentum of the SMBH binary. Our proposed mechanism causes the increase in the eccentricity of most of SMBH binaries, resulting in the rapid merger through gravitational wave radiation. Our result has given a definite solution to the 'last-parsec problem'.

  9. Microlensing Binaries with Candidate Brown Dwarf Companions

    DEFF Research Database (Denmark)

    Shin, I.-G; Han, C.; Gould, A.;

    2012-01-01

    Brown dwarfs are important objects because they may provide a missing link between stars and planets, two populations that have dramatically different formation histories. In this paper, we present the candidate binaries with brown dwarf companions that are found by analyzing binary microlensing ...

  10. Gravitational radiation, inspiraling binaries, and cosmology

    Science.gov (United States)

    Chernoff, David F.; Finn, Lee S.

    1993-01-01

    We show how to measure cosmological parameters using observations of inspiraling binary neutron star or black hole systems in one or more gravitational wave detectors. To illustrate, we focus on the case of fixed mass binary systems observed in a single Laser Interferometer Gravitational-wave Observatory (LIGO)-like detector. Using realistic detector noise estimates, we characterize the rate of detections as a function of a threshold SNR Rho(0), H0, and the binary 'chirp' mass. For Rho(0) = 8, H0 = 100 km/s/Mpc, and 1.4 solar mass neutron star binaries, the sample has a median redshift of 0.22. Under the same assumptions but independent of H0, a conservative rate density of coalescing binaries implies LIGO will observe about 50/yr binary inspiral events. The precision with which H0 and the deceleration parameter q0 may be determined depends on the number of observed inspirals. For fixed mass binary systems, about 100 observations with Rho(0) = 10 in the LIGO will give H0 to 10 percent in an Einstein-DeSitter cosmology, and 3000 will give q0 to 20 percent. For the conservative rate density of coalescing binaries, 100 detections with Rho(0) = 10 will require about 4 yrs.

  11. Gravitational waves from inspiralling binary black holes

    International Nuclear Information System (INIS)

    Binary black holes are the most promising candidate sources for the first generation of earth-based interferometric gravitational-wave detectors. We summarize and discuss the state-of-the-art analytical techniques developed during the last few years to better describe the late dynamical evolution of binary black holes of comparable masses

  12. Bayesian analysis of exoplanet and binary orbits

    CERN Document Server

    Schulze-Hartung, Tim; Henning, Thomas

    2012-01-01

    We introduce BASE (Bayesian astrometric and spectroscopic exoplanet detection and characterisation tool), a novel program for the combined or separate Bayesian analysis of astrometric and radial-velocity measurements of potential exoplanet hosts and binary stars. The capabilities of BASE are demonstrated using all publicly available data of the binary Mizar A.

  13. Planet Scattering Around Binaries: Ejections, Not Collisions

    CERN Document Server

    Smullen, Rachel A; Shannon, Andrew

    2016-01-01

    Transiting circumbinary planets discovered by Kepler provide unique insight into binary and planet formation. Several features of this new found population, for example the apparent pile-up of planets near the innermost stable orbit, may distinguish between formation theories. In this work, we determine how planet-planet scattering shapes planetary systems around binaries as compared to single stars. In particular, we look for signatures that arise due to differences in dynamical evolution in binary systems. We carry out a parameter study of N-body scattering simulations for four distinct planet populations around both binary and single stars. While binarity has little influence on the final system multiplicity or orbital distribution, the presence of a binary dramatically effects the means by which planets are lost from the system. Most circumbinary planets are lost due to ejections rather than planet-planet or planet-star collisions. The most massive planet in the system tends to control the evolution. Asid...

  14. Orbital dynamics of binary boson star systems

    International Nuclear Information System (INIS)

    We extend our previous studies of head-on collisions of boson stars by considering orbiting binary boson stars. We concentrate on equal-mass binaries and study the dynamical behavior of boson/boson and boson/antiboson pairs. We examine the gravitational wave output of these binaries and compare with other compact binaries. Such a comparison lets us probe the apparent simplicity observed in gravitational waves produced by black hole binary systems. In our system of interest however, there is an additional internal freedom which plays a significant role in the system's dynamics, namely, the phase of each star. Our evolutions show rather simple behavior at early times, but large differences occur at late times for the various initial configurations

  15. Logistic chaotic maps for binary numbers generations

    International Nuclear Information System (INIS)

    Two pseudorandom binary sequence generators, based on logistic chaotic maps intended for stream cipher applications, are proposed. The first is based on a single one-dimensional logistic map which exhibits random, noise-like properties at given certain parameter values, and the second is based on a combination of two logistic maps. The encryption step proposed in both algorithms consists of a simple bitwise XOR operation of the plaintext binary sequence with the keystream binary sequence to produce the ciphertext binary sequence. A threshold function is applied to convert the floating-point iterates into binary form. Experimental results show that the produced sequences possess high linear complexity and very good statistical properties. The systems are put forward for security evaluation by the cryptographic committees.

  16. Polarization in binary microlensing events

    CERN Document Server

    Ingrosso, G; Nucita, A A; Strafella, F; Novati, S Calchi; Jetzer, Ph; Liuzzi, G; Zakharov, A

    2013-01-01

    The light received by source stars in microlensing events may be significantly polarized if both an efficient photon scattering mechanism is active in the source stellar atmosphere and a differential magnification is therein induced by the lensing system. The best candidate events for observing polarization are highly magnified events with source stars belonging to the class of cool, giant stars {in which the stellar light is polarized by photon scattering on dust grains contained in their envelopes. The presence in the stellar atmosphere of an internal cavity devoid of dust produces polarization profiles with a two peaks structure. Hence, the time interval between them gives an important observable quantity directly related to the size of the internal cavity and to the model parameters of the lens system.} We show that {during a microlensing event} the expected polarization variability can solve an ambiguity, that arises in some cases, related to the binary or planetary lensing interpretation of the perturba...

  17. Binary Cepheids from optical interferometry

    CERN Document Server

    Gallenne, A; Mérand, A; Monnier, J D; Pietrzyński, J Breitfelder G; Gieren, W

    2013-01-01

    Classical Cepheid stars have been considered since more than a century as reliable tools to estimate distances in the universe thanks to their Period-Luminosity (P-L) relationship. Moreover, they are also powerful astrophysical laboratories, providing fundamental clues for studying the pulsation and evolution of intermediate-mass stars. When in binary systems, we can investigate the age and evolution of the Cepheid, estimate the mass and distance, and constrain theoretical models. However, most of the companions are located too close to the Cepheid (1-40 mas) to be spatially resolved with a 10-meter class telescope. The only way to spatially resolve such systems is to use long-baseline interferometry. Recently, we have started a unique and long-term interferometric program that aims at detecting and characterizing physical parameters of the Cepheid companions, with as main objectives the determination of accurate masses and geometric distances.

  18. Binary theory of electronic stopping

    CERN Document Server

    Sigmund, P

    2002-01-01

    Binary stopping theory has been developed to characterize the electronic stopping of swift heavy ions in matter. It is an extension of Bohr's classical theory of 1913 incorporating screening, higher-order-Z sub 1 and shell corrections, high-speed quantum and relativity corrections as well as projectile excitation and ionization. The main numerical input comes from optical properties. The computation of shell corrections involves orbital velocity distributions of target and projectile electrons. Calculated stopping parameters depend on ion charge. Equilibrium stopping forces may be computed by adoption of a suitable model for the equilibrium charge state. This paper summarizes the current status of the theory, in particular the sensitivity of its predictions to pertinent input. Charge-dependent stopping forces have been calculated for selected systems and compared to experimental results. Equilibrium stopping forces calculated for a wide variety of ion-target combinations are compared with experimental data fr...

  19. Binary mixtures of chiral gases

    CERN Document Server

    Presilla, Carlo

    2015-01-01

    A possible solution of the well known paradox of chiral molecules is based on the idea of spontaneous symmetry breaking. At low pressure the molecules are delocalized between the two minima of a given molecular potential while at higher pressure they become localized in one minimum due to the intermolecular dipole-dipole interactions. Evidence for such a phase transition is provided by measurements of the inversion spectrum of ammonia and deuterated ammonia at different pressures. In particular, at pressure greater than a critical value no inversion line is observed. These data are well accounted for by a model previously developed and recently extended to mixtures. In the present paper, we discuss the variation of the critical pressure in binary mixtures as a function of the fractions of the constituents.

  20. Age and growth of the scalloped hammerhead shark, Sphyrna lewini (Griffith & Smith, 1834) from the Southern coast of Sinaloa, México Edad y crecimiento del tiburón martillo, Sphyrna lewini (Griffith & Smith, 1834) de la costa sur de Sinaloa, México

    OpenAIRE

    Vicente Anislado-Tolentino; Manuel Gallardo Cabello; Felipe Amezcua Linares; Carlos Robinson Mendoza

    2008-01-01

    Age and growth for the scalloped hammerhead shark (Sphyrna lewini) were determined from opaque bands (OBs) on postcephalic vertebrae from 109 organisms (44 females, 52 cm to 276 cm total length (TL) and 65 males, 47 cm to 245 cm TL) obtained bimonthly from commercial fisheries off the southern coast of Sinaloa state (23°45'25"N and 106°05'15"W to 21°52'N and 105°54'W) from January 2003 to February 2005. The Bowker test of symmetry and the Index of Average Percent Error, suggest that this agei...

  1. Gamma-ray binaries and related systems

    CERN Document Server

    Dubus, Guillaume

    2013-01-01

    After initial claims and a long hiatus, it is now established that several binary stars emit high (0.1-100 GeV) and very high energy (>100 GeV) gamma rays. A new class has emerged called 'gamma-ray binaries', since most of their radiated power is emitted beyond 1 MeV. Accreting X-ray binaries, novae and a colliding wind binary (eta Car) have also been detected - 'related systems' that confirm the ubiquity of particle acceleration in astrophysical sources. Do these systems have anything in common ? What drives their high-energy emission ? How do the processes involved compare to those in other sources of gamma rays: pulsars, active galactic nuclei, supernova remnants ? I review the wealth of observational and theoretical work that have followed these detections, with an emphasis on gamma-ray binaries. I present the current evidence that gamma-ray binaries are driven by rotation-powered pulsars. Binaries are laboratories giving access to different vantage points or physical conditions on a regular timescale as ...

  2. Interrupted Binary Mass Transfer in Star Clusters

    CERN Document Server

    Leigh, Nathan W C; Toonen, Silvia

    2016-01-01

    Binary mass transfer is at the forefront of some of the most exciting puzzles of modern astrophysics, including Type Ia supernovae, gamma-ray bursts, and the formation of most observed exotic stellar populations. Typically, the evolution is assumed to proceed in isolation, even in dense stellar environments such as star clusters. In this paper, we test the validity of this assumption via the analysis of a large grid of binary evolution models simulated with the SeBa code. For every binary, we calculate analytically the mean time until another single or binary star comes within the mean separation of the mass-transferring binary, and compare this time-scale to the mean time for stable mass transfer to occur. We then derive the probability for each respective binary to experience a direct dynamical interruption. The resulting probability distribution can be integrated to give an estimate for the fraction of binaries undergoing mass transfer that are expected to be disrupted as a function of the host cluster pro...

  3. Pd-Si binary bulk metallic glass

    Institute of Scientific and Technical Information of China (English)

    YAO KeFu; CHEN Na

    2008-01-01

    Pd80+xSi20-x (x=0, 1, and 2) binary metallic glasses with the diameter ranging from 7 to 8 mm were prepared by a combination of fluxing and water quenching or air cooling. Thermal analysis results show that with increasing Si content, the glass transition temperature Tg, the initial crystallization temperature Tx and the onset crystalliza-tion temperature Tp of Pd-Si binary glassy alloys increase. Moreover, the super-cooled liquid region reaches 61 K. It indicates that Pd-Si binary alloys possess large glass forming ability, which can be greatly improved by fluxing treatment.

  4. Pd-Si binary bulk metallic glass

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Pd80+xSi20-x (x=0,1,and 2) binary metallic glasses with the diameter ranging from 7 to 8 mm were prepared by a combination of fluxing and water quenching or air cooling. Thermal analysis results show that with increasing Si content,the glass transition temperature Tg,the initial crystallization temperature Tx and the onset crystalliza-tion temperature Tp of Pd-Si binary glassy alloys increase. Moreover,the super-cooled liquid region reaches 61 K. It indicates that Pd-Si binary alloys possess large glass forming ability,which can be greatly improved by fluxing treatment.

  5. Eliciting Subjective Probabilities with Binary Lotteries

    DEFF Research Database (Denmark)

    Harrison, Glenn W.; Martínez-Correa, Jimmy; Swarthout, J. Todd

    2014-01-01

    We evaluate a binary lottery procedure for inducing risk neutral behavior in a subjective belief elicitation task. Prior research has shown this procedure to robustly induce risk neutrality when subjects are given a single risk task defined over objective probabilities. Drawing a sample from the...... same subject population, we find evidence that the binary lottery procedure also induces linear utility in a subjective probability elicitation task using the Quadratic Scoring Rule. We also show that the binary lottery procedure can induce direct revelation of subjective probabilities in subjects with...

  6. Cassini states for black hole binaries

    OpenAIRE

    Correia, Alexandre C. M.

    2015-01-01

    Cassini states correspond to the equilibria of the spin axis of a body when its orbit is perturbed. They were initially described for planetary satellites, but the spin axes of black hole binaries also present this kind of equilibria. In previous works, Cassini states were reported as spin-orbit resonances, but actually the spin of black hole binaries is in circulation and there is no resonant motion. Here we provide a general description of the spin dynamics of black hole binary systems base...

  7. Fast algorithms for generating binary holograms

    CERN Document Server

    Stuart, Dustin; Kuhn, Axel

    2014-01-01

    We describe three algorithms for generating binary-valued holograms. Our methods are optimised for producing large arrays of tightly focussed optical tweezers for trapping particles. Binary-valued holograms allow us to use a digital mirror device (DMD) as the display element, which is much faster than other alternatives. We describe how our binary amplitude holograms can be used to correct for phase errors caused by optical aberrations. Furthermore, we compare the speed and accuracy of the algorithms for both periodic and arbitrary arrangements of traps, which allows one to choose the ideal scheme depending on the circumstances.

  8. Potato spindle tuber viroid: the simplicity paradox resolved?

    Science.gov (United States)

    Taxonomy: Potato spindle tuber viroid (PSTVd) is the type species of the genus Posipiviroid, family Pospiviroidae. An absence of hammerhead ribozymes and the presence of a 'central conserved region' distinguish PSTVd and related viroids from members of a second viroid family, the Avsunviroidae. ...

  9. CALCULATING THE HABITABLE ZONE OF BINARY STAR SYSTEMS. II. P-TYPE BINARIES

    International Nuclear Information System (INIS)

    We have developed a comprehensive methodology for calculating the circumbinary habitable zone (HZ) in planet-hosting P-type binary star systems. We present a general formalism for determining the contribution of each star of the binary to the total flux received at the top of the atmosphere of an Earth-like planet and use the Sun's HZ to calculate the inner and outer boundaries of the HZ around a binary star system. We apply our calculations to the Kepler's currently known circumbinary planetary systems and show the combined stellar flux that determines the boundaries of their HZs. We also show that the HZ in P-type systems is dynamic and, depending on the luminosity of the binary stars, their spectral types, and the binary eccentricity, its boundaries vary as the stars of the binary undergo their orbital motion. We present the details of our calculations and discuss the implications of the results

  10. CALCULATING THE HABITABLE ZONE OF BINARY STAR SYSTEMS. II. P-TYPE BINARIES

    Energy Technology Data Exchange (ETDEWEB)

    Haghighipour, Nader [Institute for Astronomy and NASA Astrobiology Institute, University of Hawaii-Manoa, Honolulu, HI 96822 (United States); Kaltenegger, Lisa [MPIA, Koenigstuhl 17, Heidelberg, D-69117 (Germany)

    2013-11-10

    We have developed a comprehensive methodology for calculating the circumbinary habitable zone (HZ) in planet-hosting P-type binary star systems. We present a general formalism for determining the contribution of each star of the binary to the total flux received at the top of the atmosphere of an Earth-like planet and use the Sun's HZ to calculate the inner and outer boundaries of the HZ around a binary star system. We apply our calculations to the Kepler's currently known circumbinary planetary systems and show the combined stellar flux that determines the boundaries of their HZs. We also show that the HZ in P-type systems is dynamic and, depending on the luminosity of the binary stars, their spectral types, and the binary eccentricity, its boundaries vary as the stars of the binary undergo their orbital motion. We present the details of our calculations and discuss the implications of the results.

  11. Compact Binaries in Star Clusters I - Black Hole Binaries Inside Globular Clusters

    OpenAIRE

    Downing, J. M. B.; Benacquista, M. J.; Giersz, M.; Spurzem, R.

    2009-01-01

    We study the compact binary population in star clusters, focusing on binaries containing black holes, using a self-consistent Monte Carlo treatment of dynamics and full stellar evolution. We find that the black holes experience strong mass segregation and become centrally concentrated. In the core the black holes interact strongly with each other and black hole-black hole binaries are formed very efficiently. The strong interactions, however, also destroy or eject the black hole-black hole bi...

  12. Properties of planets in binary systems. The role of binary separation

    OpenAIRE

    Desidera, S.; Barbieri, M.

    2006-01-01

    The statistical properties of planets in binaries were investigated. Any difference to planets orbiting single stars can shed light on the formation and evolution of planetary systems. As planets were found around components of binaries with very different separation and mass ratio, it is particularly important to study the characteristics of planets as a function of the effective gravitational influence of the companion. A compilation of planets in binary systems was made; a search for compa...

  13. Binaries in the Hipparcos data: Keep digging

    CERN Document Server

    Pourbaix, D; Jorissen, A

    2004-01-01

    Among the 120 000 objects in the Hipparcos catalogue, only 235 were fitted with an orbital model. Besides these 235 original astrometric binaries, most Hipparcos entries with a known spectroscopic orbit (extrasolar planet or stellar companion) have now been re-processed, as part of the on-going construction of the 9th Catalogue of Spectroscopic Binary Orbits (SB9, available at http://sb9.astro.ulb.ac.be). The pitfalls and successes of this re-processing are discussed in various contexts, like (i) orbital inclinations: the holy grail for extrasolar planets (ii) searching for binaries without a priori knowledge of their spectroscopic orbital elements, and application to barium stars (iii) why not all SB9 entries yield acceptable astrometric solutions? The lessons learned from this study are useful to devise the best possible binary-detection and orbit-determination algorithms for future astrometric missions like GAIA.

  14. A mesoscopic model for binary fluids

    CERN Document Server

    Echeverria, C; Alvarez-Llamoza, O; Orozco-Guillén, E E; Morales, M; Cosenza, M G

    2016-01-01

    We propose a model to study symmetric binary fluids, based in the mesoscopic molecular simulation technique known as multiparticle collision, where space and state variables are continuous while time is discrete. We include a repulsion rule to simulate segregation processes that does not require the calculation of the interaction forces between particles, thus allowing the description of binary fluids at a mesoscopic scale. The model is conceptually simple, computationally efficient, maintains Galilean invariance, and conserves the mass and the energy in the system at micro and macro scales; while momentum is conserved globally. For a wide range of temperatures and densities, the model yields results in good agreement with the known properties of binary fluids, such as density profile, width of the interface, phase separation and phase growth. We also apply the model to study binary fluids in crowded environments with consistent results.

  15. Red-giant stars in eccentric binaries

    Directory of Open Access Journals (Sweden)

    Beck P. G.

    2015-01-01

    Full Text Available The unparalleled photometric data obtained by NASA’s Kepler Space Telescope has led to improved understanding of red-giant stars and binary stars. We discuss the characterization of known eccentric system, containing a solar-like oscillating red-giant primary component. We also report several new binary systems that are candidates for hosting an oscillating companion. A powerful approach to study binary stars is to combine asteroseimic techniques with light curve fitting. Seismology allows us to deduce the properties of red giants. In addition, by modeling the ellipsoidal modulations we can constrain the parameters of the binary system. An valuable independent source are ground-bases, high-resolution spectrographs.

  16. Evolution of Binary Stars in Multiple-Population Globular Clusters - II. Compact Binaries

    CERN Document Server

    Hong, Jongsuk; Sollima, Antonio; McMillan, Stephen L W; D'Antona, Franca; D'Ercole, Annibale

    2016-01-01

    We present the results of a survey of N-body simulations aimed at exploring the evolution of compact binaries in multiple-population globular clusters.We show that as a consequence of the initial differences in the structural properties of the first-generation (FG) and the second-generation (SG) populations and the effects of dynamical processes on binary stars, the SG binary fraction decreases more rapidly than that of the FG population. The difference between the FG and SG binary fraction is qualitatively similar to but quantitatively smaller than that found for wider binaries in our previous investigations.The evolution of the radial variation of the binary fraction is driven by the interplay between binary segregation, ionization and ejection. Ionization and ejection counteract in part the effects of mass segregation but for compact binaries the effects of segregation dominate and the inner binary fraction increases during the cluster evolution. We explore the variation of the difference between the FG an...

  17. Tidal capture formation of Low Mass X-Ray Binaries from wide binaries in the field

    CERN Document Server

    Michaely, Erez

    2015-01-01

    We present a potentially efficient dynamical formation scenario for Low Mass X-ray Binaries (LMXBs) in the field, focusing on black-hole (BH) LMXBs. In this formation channel LMXBs are formed from wide binaries $(>1000$ AU) with a BH component and a stellar companion. The wide binary is perturbed by fly-by's of field stars and its orbit random-walks and changes over time. This diffusion process can drive the binary into a sufficiently eccentric orbit such that the binary components tidally interact at peri-center and the binary evolves to become a short period binary, which eventually evolves into an LMXB. The formation rate of LMXBs through this channel mostly depends on the number of such BH wide binaries progenitors, which in turn depends on the velocity kicks imparted to BHs (or NSs) at birth. We consider several models for the formation and survival of such wide binaries, and calculate the LMXB formation rates for each model. We find that models where BHs form through direct collapse with no/little natal...

  18. Binary is Good: A Binary Inference Framework for Primary User Separation in Cognitive Radio Networks

    CERN Document Server

    Nguyen, Huy; Han, Zhu

    2010-01-01

    Primary users (PU) separation concerns with the issues of distinguishing and characterizing primary users in cognitive radio (CR) networks. We argue the need for PU separation in the context of collaborative spectrum sensing and monitor selection. In this paper, we model the observations of monitors as boolean OR mixtures of underlying binary latency sources for PUs, and devise a novel binary inference algorithm for PU separation. Simulation results show that without prior knowledge regarding PUs' activities, the algorithm achieves high inference accuracy. An interesting implication of the proposed algorithm is the ability to effectively represent n independent binary sources via (correlated) binary vectors of logarithmic length.

  19. Dixie Valley Bottoming Binary Unit

    Energy Technology Data Exchange (ETDEWEB)

    McDonald, Dale [Terra-Gen Sierra Holdings, LLC, Reno, NV (United States)

    2014-12-21

    This binary plant is the first air cooled, high-output refrigeration based waste heat recovery cycle in the industry. Its working fluid is environmentally friendly and as such, the permits that would be required with a hydrocarbon based cycle are not necessary. The unit is largely modularized, meaning that the unit’s individual skids were assembled in another location and were shipped via truck to the plant site. The Air Cooled Condensers (ACC), equipment piping, and Balance of Plant (BOP) piping were constructed at site. This project further demonstrates the technical feasibility of using low temperature brine for geothermal power utilization. The development of the unit led to the realization of low temperature, high output, and environmentally friendly heat recovery systems through domestic research and engineering. The project generates additional renewable energy, resulting in cleaner air and reduced carbon dioxide emissions. Royalty and tax payments to governmental agencies will increase, resulting in reduced financial pressure on local entities. The major components of the unit were sourced from American companies, resulting in increased economic activity throughout the country.

  20. Structure of simple (binary) oxides

    International Nuclear Information System (INIS)

    Crystal structures of different simple and binary oxides of M3O, M2O, MO, MO2, MO4, MO3, M2O3, M3O4, M2O5, M2O7 composition as well as lowest cesium oxides (Cs7O, Cs4O, Cs11O3) are considered. Cs3O crystals are constructed out of the colomns of the Cs3O composition consisting of octahedrals OCs6 jointed through the opposite faces. This is the ZrI3 ''antistructure''. Cs2O has the CdCl2 antistructure. ZrO2, HfO2, CeO2, ThO2, UO2, NpO2, PuO2, AmO2, CmO2, PoO2 oxides have the structural type of fluorite of rutile - VO2, NbO2, TaO2, MoO2, ReO2 oxides, of wurtzite - BeO. The NbO oxide is unique, in its structure the oxygen and niobium atoms form four complanar bonds. A three-dimensional skeleton constructed out of the octahedral structural units Nb6 (Nb-Nb 2.98 A) is separated. ZrO2 is a polymorphous, at 1100 grad. the monoclinic modification transfers to tetragonal. M2O7 oxides are Re2O7, Tc2O7

  1. Detecting Eccentric Globular Cluster Binaries with LISA

    OpenAIRE

    Benacquista, M.

    2001-01-01

    The energy carried in the gravitational wave signal from an eccentric binary is spread across several harmonics of the orbital frequency. The inclusion of the harmonics in the analysis of the gravitational wave signal increases the signal-to-noise ratio of the detected signal for binaries whose fundamental frequency is below the galactic confusion-limited noise cut-off. This can allow for an improved angular resolution for sources whose orbital period is greater than 2000 s. Globular cluster ...

  2. Copula-based bivariate binary response models

    OpenAIRE

    Winkelmann, Rainer

    2009-01-01

    The bivariate probit model is frequently used for estimating the effect of an endogenous binary regressor on a binary outcome variable. This paper discusses simple modifications that maintain the probit assumption for the marginal distributions while introducing non-normal dependence among the two variables using copulas. Simulation results and evidence from two applications, one on the effect of insurance status on ambulatory expenditure and one on the effect of completing high school on sub...

  3. Binary compact object inspiral: Detection expectations

    Indian Academy of Sciences (India)

    Vassiliki Kalogera

    2004-10-01

    We review the current estimates of binary compact object inspiral rates in particular in view of the recently discovered highly relativistic binary pulsar J0737-3039. One of the robust results is that, because of this discovery, the rate estimates for binary neutron stars have increased by a factor of 6-7 independent of any uncertainties related to the pulsar population properties. This rate increase has dramatic implications for gravitational wave detectors. For initial LIGO, the most probable detection rates for double neutron star (DNS) inspirals is 1 event/(5{250) yr; at 95% confidence we obtain rates up to 1/1.5 yr. For advanced LIGO, the most probable rates are 20-1000 events/yr. These predictions, for the first time, bring the expectations for DNS detections by initial LIGO to the astrophysically relevant regime. We also use our models to predict that the large-scale Parkes multibeam pulsar survey with acceleration searches could detect an average of three to four binary pulsars similar to those known at present. In comparison, rate estimates for binaries with black holes are derived based on binary evolution calculation, and based on the optimistic ends of the ranges, remain an important candidate for inspiral detection in the next few years. We also consider another aspect of the detectability of binary inspiral: the effect of precession on the detection efficiency of astrophysically relevant binaries. Based on our current astrophysical expectations, large tilt angles are not favored. As a result the decrease in detection rate varies rather slowly with black hole spin magnitude and is within 20-30% of the maximum possible values.

  4. Planet Scattering Around Binaries: Ejections, Not Collisions

    OpenAIRE

    Smullen, Rachel A.; Kratter, Kaitlin M.; Shannon, Andrew

    2016-01-01

    Transiting circumbinary planets discovered by Kepler provide unique insight into binary and planet formation. Several features of this new found population, for example the apparent pile-up of planets near the innermost stable orbit, may distinguish between formation theories. In this work, we determine how planet-planet scattering shapes planetary systems around binaries as compared to single stars. In particular, we look for signatures that arise due to differences in dynamical evolution in...

  5. GAIA survey of galactic eclipsing binaries

    OpenAIRE

    Zwitter, Tomaz

    2002-01-01

    General importance and capabilities of observations of eclipsing binaries by the forthcoming ESA mission GAIA are discussed. Availability of spectroscopic observations and a large number of photometric bands on board will make it possible to reliably determine physical parameters for $\\sim 10^5$ binary stars. It is stressed that current methods of object by object analysis will have to be modified and included in an automatic analysis pipeline.

  6. Formation of Compact Binaries in Globular Clusters

    OpenAIRE

    Rappaport, Saul; Pfahl, Eric; Rasio, Fred; Podsiadlowski, Philipp

    2001-01-01

    We report here on two complementary population synthesis studies which relate directly to the formation and evolution of neutron star binaries in globular clusters. In the first, we compute the probability of retaining neutron stars in globular clusters, and quantitatively confirm the idea that the retention fraction for neutron stars born in binary systems is greatly enhanced over those born in isolated stars. However, the retention fraction may well be insufficient to explain the current po...

  7. Diffusion in ordered binary solid systems

    International Nuclear Information System (INIS)

    This thesis contains contributions to the field of diffusion in ordered binary solid systems. An extensive experimental investigation of the self diffusion in CoGa is presented. The results of these diffusion measurements strongly suggest that a substantial part of the atomic migration is caused by a new type of defect. A quantitative description of the atomic displacements via this defect is given. Finally computer simulations are presented of diffusion and ordering in binary solid systems. (Auth.)

  8. Binary nature of the Barium stars

    International Nuclear Information System (INIS)

    We present radial-velocity spectrometer observations that indicate that Ba II stars are binary systems. The secondary stars of these systems have low masses, consistent with their being degenerate objects which have lost mass onto their primaries in a previous stage of evolution. It is suggested that the Population II equivalents, the CH stars, may also be binary systems. This may be related to the fact that they are found only in globular clusters of the lowest central concentration

  9. On homogeneous nontransitive binary perfect code

    OpenAIRE

    Mogilnykh, I. Yu.; Solov'eva, F. I.

    2014-01-01

    Studying binary perfect codes we show the existence of homogeneous nontransitive codes. Thus, as far as perfect codes are concerned, the propelinear codes are strictly contained in transitive codes, wheresas homogeneous codes form a strict subclass of transitive codes. In the work we deduce a necessary and sufficient condition for transitivity of perfect binary codes of rank one more than that of Hamming code. The paper is in Russian.

  10. Texture classification by texton: statistical versus binary.

    Science.gov (United States)

    Guo, Zhenhua; Zhang, Zhongcheng; Li, Xiu; Li, Qin; You, Jane

    2014-01-01

    Using statistical textons for texture classification has shown great success recently. The maximal response 8 (Statistical_MR8), image patch (Statistical_Joint) and locally invariant fractal (Statistical_Fractal) are typical statistical texton algorithms and state-of-the-art texture classification methods. However, there are two limitations when using these methods. First, it needs a training stage to build a texton library, thus the recognition accuracy will be highly depended on the training samples; second, during feature extraction, local feature is assigned to a texton by searching for the nearest texton in the whole library, which is time consuming when the library size is big and the dimension of feature is high. To address the above two issues, in this paper, three binary texton counterpart methods were proposed, Binary_MR8, Binary_Joint, and Binary_Fractal. These methods do not require any training step but encode local feature into binary representation directly. The experimental results on the CUReT, UIUC and KTH-TIPS databases show that binary texton could get sound results with fast feature extraction, especially when the image size is not big and the quality of image is not poor. PMID:24520346

  11. Texture classification by texton: statistical versus binary.

    Directory of Open Access Journals (Sweden)

    Zhenhua Guo

    Full Text Available Using statistical textons for texture classification has shown great success recently. The maximal response 8 (Statistical_MR8, image patch (Statistical_Joint and locally invariant fractal (Statistical_Fractal are typical statistical texton algorithms and state-of-the-art texture classification methods. However, there are two limitations when using these methods. First, it needs a training stage to build a texton library, thus the recognition accuracy will be highly depended on the training samples; second, during feature extraction, local feature is assigned to a texton by searching for the nearest texton in the whole library, which is time consuming when the library size is big and the dimension of feature is high. To address the above two issues, in this paper, three binary texton counterpart methods were proposed, Binary_MR8, Binary_Joint, and Binary_Fractal. These methods do not require any training step but encode local feature into binary representation directly. The experimental results on the CUReT, UIUC and KTH-TIPS databases show that binary texton could get sound results with fast feature extraction, especially when the image size is not big and the quality of image is not poor.

  12. Direct Exoplanet Detection with Binary Differential Imaging

    CERN Document Server

    Rodigas, Timothy J; Mamajek, Eric E; Males, Jared R; Close, Laird M; Morzinski, Katie; Hinz, Philip M; Kaib, Nathan

    2015-01-01

    Binaries are typically excluded from direct imaging exoplanet surveys. However, the recent findings of Kepler and radial velocity programs show that planets can and do form in binary systems. Here, we suggest that visual binaries offer unique advantages for direct imaging. We show that Binary Differential Imaging (BDI), whereby two stars are imaged simultaneously at the same wavelength within the isoplanatic patch at high Strehl ratio, offers improved point spread function (PSF) subtraction that can result in increased sensitivity to planets close to each star. We demonstrate this by observing a young visual binary separated by 4\\asec ~with MagAO/Clio-2 at 3.9 \\microns, where the Strehl ratio is high, the isoplanatic patch is large, and giant planets are bright. Comparing BDI to angular differential imaging (ADI), we find that BDI's 5$\\sigma$ contrast is \\about 0.5 mags better than ADI's within \\about 1\\asec ~for the particular binary we observed. Because planets typically reside close to their host stars, BD...

  13. Spectroscopic Orbits for Kepler FOV Eclipsing Binaries

    Science.gov (United States)

    Matson, Rachel A.; Gies, Douglas R.; Williams, Stephen J.; Guo, Zhao

    2013-02-01

    We are currently involved in a four year program of precise eclipsing binary photometry with the NASA Kepler Observatory. Our goal is to search for variations in minimum light timing for intermediate mass eclipsing binaries. Such periodic variations will reveal the reflex motion caused by any distant, low mass object that orbits the close binary. it Kepler's unprecedented accuracy and continuous observations provide a unique opportunity to detect the low mass companions that are predicted to result from the angular momentum of the natal cloud. The goal of this proposal is to obtain blue spectra of short period (0.9-6d) eclipsing binaries, derive radial velocities, and produce a double-lined spectroscopic orbit (as well as estimates of the stellar effective temperatures, gravities, and metallicities). Combined with the it Kepler light curve, we will determine very accurate masses and radii for the members of the close binary, which will yield the mass-inclination product M_3 sin i for any companions detected by light travel time or other effects. An extended sample of eclipsing binaries with longer periods (up to 50d) is now being investigated to test whether the presence of a tertiary companion declines with increasing period. We propose to obtain a single spectrum at quadrature for the brightest 48 stars in this expanded sample to characterize the effective temperatures and total mass contained in these systems.

  14. Stability and Coalescence of Massive Twin Binaries

    CERN Document Server

    Hwang, Jason A; Rasio, Frederic A; Kalogera, Vassiliki

    2015-01-01

    Massive stars are usually found in binaries, and binaries with periods less than 10 days may have a preference for near equal component masses. In this paper we investigate the evolution of these binaries all the way to contact and the possibility that these systems can be progenitors of double neutron star binaries. The small orbital separations of observed double neutron star binaries suggest that the progenitor systems underwent a common envelope phase at least once during their evolution. Bethe & Brown (1998) proposed that massive binary twins will undergo a common envelope evolution while both components are ascending the red giant branch or asymptotic giant branch simultaneously, also known as double-core evolution. Using models generated from the stellar evolution code Evolve Zero Age Main Sequence, we determine the range of mass ratios resulting in both components simultaneously ascending the RGB or AGB as a function of the difference in birth times, t. We find that, even for a generous t=5 Myr, t...

  15. Spectroscopic subsystems in nearby wide binaries

    CERN Document Server

    Tokovinin, Andrei

    2015-01-01

    Radial velocity (RV) monitoring of solar-type visual binaries has been conducted at the CTIO/SMARTS 1.5-m telescope to study short-period systems. Data reduction is described, mean and individual RVs of 163 observed objects are given. New spectroscopic binaries are discovered or suspected in 17 objects, for some of them orbital periods could be determined. Subsystems are efficiently detected even in a single observation by double lines and/or by the RV difference between the components of visual binaries. The potential of this detection technique is quantified by simulation and used for statistical assessment of 96 wide binaries within 67pc. It is found that 43 binaries contain at least one subsystem and the occurrence of subsystems is equally probable in either primary or secondary components. The frequency of subsystems and their periods match the simple prescription proposed by the author (2014, AJ, 147, 87). The remaining 53 simple wide binaries with a median projected separation of 1300AU have the distri...

  16. Design and Implementation of BDB, the Binary Star Database

    Science.gov (United States)

    Kaygorodov, P.; Kovaleva, D.; Malkov, O.

    2013-02-01

    The Binary star DataBase (BDB, http://bdb.inasan.ru) is created to provide liasons between binary star catalogue data of various origin. Information on different observational types of binaries is obtained from heterogeneous sources of data - astronomical catalogues and surveys. The database allows a variety of query options useful for selected stars investigation purposes, for binary observations planning, and for construction and examination of binary datasets with certain characteristics.

  17. Dual-selection for evolution of in vivo functional aptazymes as riboswitch parts.

    Science.gov (United States)

    Goler, Jonathan A; Carothers, James M; Keasling, Jay D

    2014-01-01

    Both synthetic biology and metabolic engineering are aided by the development of genetic control parts. One class of riboswitch parts that has great potential for sensing and regulation of protein levels is aptamer-coupled ribozymes (aptazymes). These devices are comprised of an aptamer domain selected to bind a particular ligand, a ribozyme domain, and a communication module that regulates the ribozyme activity based on the state of the aptamer. We describe a broadly applicable method for coupling a novel, newly selected aptamer to a ribozyme to generate functional aptazymes via in vitro and in vivo selection. To illustrate this approach, we describe experimental procedures for selecting aptazymes assembled from aptamers that bind p-amino-phenylalanine and a hammerhead ribozyme. Because this method uses selection, it does not rely on sequence-specific design and thus should be generalizable for the generation of in vivo operational aptazymes that respond to any targeted molecules. PMID:24549623

  18. Exploring the Birth of Binary Stars

    Science.gov (United States)

    Kohler, Susanna

    2016-08-01

    More than half of all stars are thought to be in binary or multiple star systems. But how do these systems form? The misaligned spins of some binary protostars might provide a clue.Two Formation ModelsIts hard to tell how multiple-star systems form, since these systems are difficult to observe in their early stages. But based on numerical simulations, there are two proposed models for the formation of stellar binaries:Turbulent fragmentationTurbulence within a single core leads to multiple dense clumps. These clumps independently collapse to form stars that orbit each other.Disk fragmentationGravitational instabilities in a massive accretion disk cause the formation of a smaller, secondary disk within the first, resulting in two stars that orbit each other.Log column density for one of the authors simulated binary systems, just after the formation of two protostars. Diamonds indicate the protostar positions. [Adapted from Offner et al. 2016]Outflows as CluesHow can we differentiate between these formation mechanisms? Led by Stella Offner (University of Massachusetts), a team of scientists has suggested that the key isto examine the alignment of the stars protostellar outflows jets that are often emitted from the poles of young, newly forming stars.Naively, wed expect that disk fragmentation would produce binary stars with common angular momentum. As the stars spins would be aligned, they would therefore also launch protostellar jets that were aligned with each other. Turbulent fragmentation, on the other hand, would cause the stars to have independent angular momentum. This would lead to randomly oriented spins, so the protostellar jets would be misaligned.Snapshots from the authors simulations. Left panel of each pair: column density; green arrows giveprotostellar spin directions. Right panel: synthetic observations produced from the simulations; cyan arrows giveprotostellar outflow directions. [Offner et al. 2016]Simulations of FragmentationIn order to better

  19. New spectroscopic binary companions of giant stars and updated metallicity distribution for binary systems

    CERN Document Server

    Bluhm, P; Vanzi, L; Soto, M G; Vos, J; Wittenmyer, R A; Olivares, F; Drass, H; Mennickent, R E; Vuckovic, M; Rojo, P; Melo, C H F

    2016-01-01

    We report the discovery of 24 spectroscopic binary companions to giant stars. We fully constrain the orbital solution for 6 of these systems. We cannot unambiguously derive the orbital elements for the remaining stars because the phase coverage is incomplete. Of these stars, 6 present radial velocity trends that are compatible with long-period brown dwarf companions.The orbital solutions of the 24 binary systems indicate that these giant binary systems have a wide range in orbital periods, eccentricities, and companion masses. For the binaries with restricted orbital solutions, we find a range of orbital periods of between $\\sim$ 97-1600 days and eccentricities of between $\\sim$ 0.1-0.4. In addition, we studied the metallicity distribution of single and binary giant stars. We computed the metallicity of a total of 395 evolved stars, 59 of wich are in binary systems. We find a flat distribution for these binary stars and therefore conclude that stellar binary systems, and potentially brown dwarfs, have a diffe...

  20. Towards the field binary population: Influence of orbital decay on close binaries

    CERN Document Server

    Korntreff, Christina; Pfalzner, Susanne

    2012-01-01

    Surveys of the binary populations in the solar neighbourhood have shown that the periods of G- and M-type stars are log-normally distributed. However, observations of young binary populations suggest a log-uniform distribution. Clearly some process(es) change the period distribution over time. Most stars form in star clusters, in which two important dynamical processes occur: i) gas-induced orbital decay of embedded binary systems and ii) destruction of soft binaries in three-body interactions. The emphasis here is on orbital decay which has been largely neglected so far. Using a combination of Monte-Carlo and dynamical nbody modelling it is demonstrated here that the cluster dynamics destroys the number of wide binaries, but leaves short-period binaries basically undisturbed even for a initially log-uniform distribution. By contrast orbital decay significantly reduces the number and changes the properties of short-period binaries, but leaves wide binaries largely uneffected. Until now it was unclear whether ...

  1. Gravitational wave background from binary systems

    International Nuclear Information System (INIS)

    Basic aspects of the background of gravitational waves and its mathematical characterization are reviewed. The spectral energy density parameter Ω(f), commonly used as a quantifier of the background, is derived for an ensemble of many identical sources emitting at different times and locations. For such an ensemble, Ω(f) is generalized to account for the duration of the signals and of the observation, so that one can distinguish the resolvable and unresolvable parts of the background. The unresolvable part, often called confusion noise or stochastic background, is made by signals that cannot be either individually identified or subtracted out of the data. To account for the resolvability of the background, the overlap function is introduced. This function is a generalization of the duty cycle, which has been commonly used in the literature, in some cases leading to incorrect results. The spectra produced by binary systems (stellar binaries and massive black hole binaries) are presented over the frequencies of all existing and planned detectors. A semi-analytical formula for Ω(f) is derived in the case of stellar binaries (containing white dwarfs, neutron stars or stellar-mass black holes). Besides a realistic expectation of the level of background, upper and lower limits are given, to account for the uncertainties in some astrophysical parameters such as binary coalescence rates. One interesting result concerns all current and planned ground-based detectors (including the Einstein Telescope). In their frequency range, the background of binaries is resolvable and only sporadically present. In other words, there is no stochastic background of binaries for ground-based detectors.

  2. Birth of Massive Black Hole Binaries

    Energy Technology Data Exchange (ETDEWEB)

    Colpi, M.; /Milan Bicocca U.; Dotti, M.; /Insubria U., Como; Mayer, L.; /Zurich, ETH; Kazantzidis, S.; /KIPAC, Menlo Park

    2007-11-19

    If massive black holes (BHs) are ubiquitous in galaxies and galaxies experience multiple mergers during their cosmic assembly, then BH binaries should be common albeit temporary features of most galactic bulges. Observationally, the paucity of active BH pairs points toward binary lifetimes far shorter than the Hubble time, indicating rapid inspiral of the BHs down to the domain where gravitational waves lead to their coalescence. Here, we review a series of studies on the dynamics of massive BHs in gas-rich galaxy mergers that underscore the vital role played by a cool, gaseous component in promoting the rapid formation of the BH binary. The BH binary is found to reside at the center of a massive self-gravitating nuclear disc resulting from the collision of the two gaseous discs present in the mother galaxies. Hardening by gravitational torques against gas in this grand disc is found to continue down to sub-parsec scales. The eccentricity decreases with time to zero and when the binary is circular, accretion sets in around the two BHs. When this occurs, each BH is endowed with it own small-size ({approx}< 0.01 pc) accretion disc comprising a few percent of the BH mass. Double AGN activity is expected to occur on an estimated timescale of {approx}< 1 Myr. The double nuclear point-like sources that may appear have typical separation of {approx}< 10 pc, and are likely to be embedded in the still ongoing starburst. We note that a potential threat of binary stalling, in a gaseous environment, may come from radiation and/or mechanical energy injections by the BHs. Only short-lived or sub-Eddington accretion episodes can guarantee the persistence of a dense cool gas structure around the binary necessary for continuing BH inspiral.

  3. Massive gaseous discs around SMBH binaries: Binary decay and tidal disruptions

    Directory of Open Access Journals (Sweden)

    Brem P.

    2012-12-01

    Full Text Available We investigate the evolution of black hole binaries embedded within geometrically thin gas discs. Our results imply that such discs can produce black hole mergers for relatively low-mass binaries, and that a significant population of eccentric binaries might exist at separations of a few 0.01 pc. These binaries may be detectable due to the time-variable accretion on to the black holes. If the disc fragments, then the newly-born stars will continue driving the binary to its coalescence, although at a slower rate. Interestingly, our preliminary analysis shows that these stars will be disrupted at a rate of ∼10−4–2 · 10−5 events per year per system.

  4. Serial binary interval ratios improve rhythm reproduction

    Directory of Open Access Journals (Sweden)

    XiangWu

    2013-08-01

    Full Text Available Musical rhythm perception is a natural human ability that involves complex cognitive processes. Rhythm refers to the organization of events in time, and musical rhythms have an underlying hierarchical metrical structure. The metrical structure induces the feeling of a beat and the extent to which a rhythm induces the feeling of a beat is referred to as its metrical strength. Binary ratios are the most frequent interval ratio in musical rhythms. Rhythms with hierarchical binary ratios are better discriminated and reproduced than rhythms with hierarchical non-binary ratios. However, it remains unclear whether a superiority of serial binary over non-binary ratios in rhythm perception and reproduction exists. In addition, how different types of serial ratios influence the metrical strength of rhythms remains to be elucidated. The present study investigated serial binary vs. non-binary ratios in a reproduction task. Rhythms formed with exclusively binary (1:2:4:8, non-binary integer (1:3:5:6, and non-integer (1:2.3:5.3:6.4 ratios were examined within a constant meter. The results showed that the 1:2:4:8 rhythm type was more accurately reproduced than the 1:3:5:6 and 1:2.3:5.3:6.4 rhythm types, and the 1:2.3:5.3:6.4 rhythm type was more accurately reproduced than the 1:3:5:6 rhythm type. Further analyses showed that reproduction performance was better predicted by the distribution pattern of event occurrences within an inter-beat interval, than by the coincidence of events with beats, or the magnitude and complexity of interval ratios. Whereas rhythm theories and empirical data emphasize the role of the coincidence of events with beats in determining metrical strength and predicting rhythm performance, the present results suggest that rhythm processing may be better understood when the distribution pattern of event occurrences is taken into account. These results provide new insights into the mechanisms underlining musical rhythm perception.

  5. Urey Prize Lecture: Binary Minor Planets

    Science.gov (United States)

    Margot, J. L.

    2004-11-01

    The discovery of binary systems in the near-Earth, main belt, and Kuiper belt populations provides an abundance of new data that expand our knowledge of the physics and chemistry of the solar system. Binary minor planets form as a result of collisional, tidal, and capture processes that are important to study as they play major roles in the formation and evolution of planetary systems. The frequency of occurrence of such processes directly reflects the dynamical environment in the various populations. Observations of binaries provide a powerful way to measure the bulk properties of small bodies, which in turn lead to inferences about their composition and internal structure. These data may offer a rare glimpse of what physical and chemical conditions prevailed when protoplanets formed, and what subsequent evolution took place. In the case of the Kuiper Belt, the study of a handful of binaries forces us to rethink how dense and how bright these bodies are, and to significantly revise our current mass estimates for the entire population. The number of known binary minor planets has increased dramatically over the past few years, with roughly ten new discoveries each year. I will attempt to summarize recent developments, with examples drawn from my observations with the Hubble, Palomar, Keck, Arecibo and Goldstone telescopes.

  6. Orbital eccentricities in primordial black holes binaries

    CERN Document Server

    Cholis, Ilias; Ali-Haïmoud, Yacine; Bird, Simeon; Kamionkowski, Marc; Muñoz, Julian B; Raccanelli, Alvise

    2016-01-01

    It was recently suggested that the merger of $\\sim30\\,M_\\odot$ primordial black holes (PBHs) may provide a significant number of events in gravitational-wave observatories over the next decade, if they make up an appreciable fraction of the dark matter. Here we show that measurement of the eccentricities of the inspiralling binary black holes can be used to distinguish these binaries from those produced by more traditional astrophysical mechanisms. These PBH binaries are formed on highly eccentric orbits and can then merge on timescales that in some cases are years or less, retaining some eccentricity in the last seconds before the merger. This is to be contrasted with massive-stellar-binary, globular-cluster, or other astrophysical origins for binary black holes (BBHs) in which the orbits have very effectively circularized by the time the BBH enters the observable LIGO window. Here we discuss the features of the gravitational-wave signals that indicate this eccentricity and forecast the sensitivity of LIGO a...

  7. Spherical hashing: binary code embedding with hyperspheres.

    Science.gov (United States)

    Heo, Jae-Pil; Lee, Youngwoon; He, Junfeng; Chang, Shih-Fu; Yoon, Sung-Eui

    2015-11-01

    Many binary code embedding schemes have been actively studied recently, since they can provide efficient similarity search, and compact data representations suitable for handling large scale image databases. Existing binary code embedding techniques encode high-dimensional data by using hyperplane-based hashing functions. In this paper we propose a novel hypersphere-based hashing function, spherical hashing, to map more spatially coherent data points into a binary code compared to hyperplane-based hashing functions. We also propose a new binary code distance function, spherical Hamming distance, tailored for our hypersphere-based binary coding scheme, and design an efficient iterative optimization process to achieve both balanced partitioning for each hash function and independence between hashing functions. Furthermore, we generalize spherical hashing to support various similarity measures defined by kernel functions. Our extensive experiments show that our spherical hashing technique significantly outperforms state-of-the-art techniques based on hyperplanes across various benchmarks with sizes ranging from one to 75 million of GIST, BoW and VLAD descriptors. The performance gains are consistent and large, up to 100 percent improvements over the second best method among tested methods. These results confirm the unique merits of using hyperspheres to encode proximity regions in high-dimensional spaces. Finally, our method is intuitive and easy to implement. PMID:26440269

  8. An interferometric view of binary stars

    CERN Document Server

    Boffin, Henri M J

    2016-01-01

    The study of binary stars is critical to apprehend many of the most interesting classes of stars. Moreover, quite often, the study of stars in binary systems is our only mean to constrain stellar properties, such as masses and radii. Unfortunately, a great fraction of the most interesting binaries are so compact that they can only be apprehended by high-resolution techniques, mostly by interferometry. I present some results highlighting the use of interferometry in the study of binary stars, from finding companions and deriving orbits, determining the mass and radius of stars, to studying mass transfer in symbiotic stars, and tackling luminous blue variables. In particular, I show how interferometric studies using the PIONIER instrument have allowed us to confirm a dichotomy within symbiotic stars, obtain masses of stars with a precision better than 1%, and help us find a new Eta Carinae-like system. I will also illustrate the benefits for the study of binary stars one would get from upgrading the VLT Interfe...

  9. Planet Scattering Around Binaries: Ejections, Not Collisions

    Science.gov (United States)

    Smullen, Rachel A.; Kratter, Kaitlin M.; Shannon, Andrew

    2016-06-01

    Transiting circumbinary planets discovered by Kepler provide unique insight into binary star and planet formation. Several features of this new found population, for example the apparent pile-up of planets near the innermost stable orbit, may distinguish between formation theories. In this work, we determine how planet-planet scattering shapes planetary systems around binaries as compared to single stars. In particular, we look for signatures that arise due to differences in dynamical evolution in binary systems. We carry out a parameter study of N-body scattering simulations for four distinct planet populations around both binary and single stars. While binarity has little influence on the final system multiplicity or orbital distribution, the presence of a binary dramatically effects the means by which planets are lost from the system. Most circumbinary planets are lost due to ejections rather than planet-planet or planet-star collisions. The most massive planet in the system tends to control the evolution. Systems similar to the only observed multi-planet circumbinary system, Kepler-47, can arise from much more tightly packed, unstable systems. Only extreme initial conditions introduce differences in the final planet populations. Thus, we suggest that any intrinsic differences in the populations are imprinted by formation.

  10. Investigating Dark Energy with Black Hole Binaries

    International Nuclear Information System (INIS)

    The accelerated expansion of the universe is ascribed to the existence of dark energy. Black holes accrete dark energy. The accretion induces a mass change proportional to the energy density and pressure of the background dark energy fluid. The time scale during which the mass of black holes changes considerably is long relative to the age of the universe, thus beyond detection possibilities. We propose to take advantage of the modified black hole masses for exploring the equation of state w[z] of dark energy, by investigating the evolution of supermassive black hole binaries on a dark energy background. Deriving the signatures of dark energy accretion on the evolution of binaries, we find that dark energy imprints on the emitted gravitational radiation and on the changes in the orbital radius of the binary can be within detection limits for certain supermassive black hole binaries. This talk describes how binaries can provide a useful tool in obtaining complementary information on the nature of dark energy.

  11. INTEGRAL & RXTE View of Gamma-ray Binaries

    OpenAIRE

    Jian LI; Torres, Diego F.; Zhang, Shu; WANG, JIANMIN

    2013-01-01

    Gamma-ray binaries are X-ray binaries with gamma-ray emissions. Their multi-wavelength emissions range from radio, optical, X-ray and to very high energy (TeV). X-ray emissions are crucial to understand the nature of gamma-ray binaries. INTEGRAL and RXTE have covered and monitored most of the gamma-ray binaries in hard and soft X-rays. Here we report the results of several gamma-ray binaries and possible gamma-ray binaries from INTEGRAL and RXTE.

  12. The Circulation Pattern in Simulated Contact Binaries

    Science.gov (United States)

    Motl, Patrick M.; Frank, J.; Tohline, J. E.

    2006-06-01

    We present a three-dimensional hydrodynamical simulation of an initially symmetric (equal mass) binary where both components are marginally in contact. The simulation evolves the binary through approximately 150 orbital periods and within the first 20 orbits, a global velocity field is established that carries material between both components. In the equatorial plane, the flow is along a figure eight pattern with streams of material sliding past one another in the neighborhood of the inner Lagrange point. For our chosen equation of state, mass transfer is ultimately unstable in this binary though the growth time is long compared to the orbital period. We are therefore able to observe that the circulation pattern, once established, is quite close to steady state. We explore the role that similar steady state flows may play in real contact systems.

  13. TIDAL NOVAE IN COMPACT BINARY WHITE DWARFS

    International Nuclear Information System (INIS)

    Compact binary white dwarfs (WDs) undergoing orbital decay due to gravitational radiation can experience significant tidal heating prior to merger. In these WDs, the dominant tidal effect involves the excitation of outgoing gravity waves in the inner stellar envelope and the dissipation of these waves in the outer envelope. As the binary orbit decays, the WDs are synchronized from outside in (with the envelope synchronized first, followed by the core). We examine the deposition of tidal heat in the envelope of a carbon-oxygen WD and study how such tidal heating affects the structure and evolution of the WD. We show that significant tidal heating can occur in the star's degenerate hydrogen layer. This layer heats up faster than it cools, triggering runaway nuclear fusion. Such 'tidal novae' may occur in all WD binaries containing a CO WD, at orbital periods between 5 minutes and 20 minutes, and precede the final merger by 105-106 years.

  14. Gravitational waves from spinning eccentric binaries

    CERN Document Server

    Csizmadia, Péter; Rácz, István; Vasúth, Mátyás

    2012-01-01

    This paper is to introduce a new software called CBwaves which provides a fast and accurate computational tool to determine the gravitational waveforms yielded by generic spinning binaries of neutron stars and/or black holes on eccentric orbits. This is done within the post-Newtonian (PN) framework by integrating the equations of motion and the spin precession equations while the radiation field is determined by a simultaneous evaluation of the analytic waveforms. In applying CBwaves various physically interesting scenarios have been investigated. In particular, we have studied the appropriateness of the adiabatic approximation, and justified that the energy balance relation is indeed insensitive to the specific form of the applied radiation reaction term. By studying eccentric binary systems it is demonstrated that circular template banks are very ineffective in identifying binaries even if they possess tiny residual orbital eccentricity. In addition, by investigating the validity of the energy balance relat...

  15. Binary Particle Model of Weak Interactions

    CERN Document Server

    Ndili, F N

    2011-01-01

    We introduce the new concept of binary particle as the basic matter unit that participates in weak interactions and not any one fermion singly. We state the quantum numbers of this binary particle, and show the concept leads us to a natural explanation of the standard model puzzle of the origin of flavor mixing and the CKM matrix. Certain other puzzles of the standard model such as the absence of flavor changing neutral currents (FCNC), are also explained naturally by the binary particle model. These puzzles are currently thought to be esoteric properties of electro weak interactions that have origins in physics beyond the standard model at some ultra high energy scales. We show that this is not necessarily the case.

  16. A binary spelling interface with random errors.

    Science.gov (United States)

    Perelmouter, J; Birbaumer, N

    2000-06-01

    An algorithm for design of a spelling interface based on a modified Huffman's algorithm is presented. This algorithm builds a full binary tree that allows to maximize an average probability to reach a leaf where a required character is located when a choice at each node is made with possible errors. A means to correct errors (a delete-function) and an optimization method to build this delete-function into the binary tree are also discussed. Such a spelling interface could be successfully applied to any menu-orientated alternative communication system when a user (typically, a patient with devastating neuromuscular handicap) is not able to express an intended single binary response, either through motor responses or by using of brain-computer interfaces, with an absolute reliability. PMID:10896195

  17. Hybrid Black-Hole Binary Initial Data

    Science.gov (United States)

    Mundim, Bruno C.; Kelly, Bernard J.; Nakano, Hiroyuki; Zlochower, Yosef; Campanelli, Manuela

    2010-01-01

    "Traditional black-hole binary puncture initial data is conformally flat. This unphysical assumption is coupled with a lack of radiation signature from the binary's past life. As a result, waveforms extracted from evolutions of this data display an abrupt jump. In Kelly et al. [Class. Quantum Grav. 27:114005 (2010)], a new binary black-hole initial data with radiation contents derived in the post-Newtonian (PN) calculations was adapted to puncture evolutions in numerical relativity. This data satisfies the constraint equations to the 2.5PN order, and contains a transverse-traceless "wavy" metric contribution, violating the standard assumption of conformal flatness. Although the evolution contained less spurious radiation, there were undesired features; the unphysical horizon mass loss and the large initial orbital eccentricity. Introducing a hybrid approach to the initial data evaluation, we significantly reduce these undesired features."

  18. Modeling Flows Around Merging Black Hole Binaries

    CERN Document Server

    van Meter, James R; Miller, M Coleman; Reynolds, Christopher S; Centrella, Joan M; Baker, John G; Boggs, William D; Kelly, Bernard J; McWilliams, Sean T

    2009-01-01

    Coalescing massive black hole binaries are produced by the mergers of galaxies. The final stages of the black hole coalescence produce strong gravitational radiation that can be detected by the space-borne LISA. In cases where the black hole merger takes place in the presence of gas and magnetic fields, various types of electromagnetic signals may also be produced. Modeling such electromagnetic counterparts of the final merger requires evolving the behavior of both gas and fields in the strong-field regions around the black holes. We have taken a step towards solving this problem by mapping the flow of pressureless matter in the dynamic, 3-D general relativistic spacetime around the merging black holes. We find qualitative differences in collision and outflow speeds, including a signature of the merger when the net angular momentum of the matter is low, between the results from single and binary black holes, and between nonrotating and rotating holes in binaries. If future magnetohydrodynamic results confirm ...

  19. Nonlinear Tides in Close Binary Systems

    CERN Document Server

    Weinberg, Nevin N; Quataert, Eliot; Burkart, Josh

    2011-01-01

    We study the excitation and damping of tides in close binary systems, accounting for the leading order nonlinear corrections to linear tidal theory. These nonlinear corrections include two distinct effects: three-mode nonlinear interactions and nonlinear excitation of modes by the time-varying gravitational potential of the companion. This paper presents the formalism for studying nonlinear tides and studies the nonlinear stability of the linear tidal flow. Although the formalism is applicable to binaries containing stars, planets, or compact objects, we focus on solar type stars with stellar or planetary companions. Our primary results include: (1) The linear tidal solution often used in studies of binary evolution is unstable over much of the parameter space in which it is employed. More specifically, resonantly excited gravity waves are unstable to parametric resonance for companion masses M' > 10-100 M_Earth at orbital periods P = 1-10 days. The nearly static equilibrium tide is, however, parametrically s...

  20. Gravitational wave background from binary systems

    CERN Document Server

    Rosado, Pablo A

    2011-01-01

    Basic aspects of the background of gravitational waves and its mathematical characterization are reviewed. The spectral energy density parameter $\\Omega(f)$, commonly used as a quantifier of the background, is derived for an ensemble of many identical sources emitting at different times and locations. For such an ensemble, $\\Omega(f)$ is generalized to account for the duration of the signals and of the observation, so that one can distinguish the resolvable and unresolvable parts of the background. The unresolvable part, often called confusion noise or stochastic background, is made by signals that cannot be either individually identified or subtracted out of the data. To account for the resolvability of the background, the overlap function is introduced. This function is a generalization of the duty cycle, which has been commonly used in the literature, in some cases leading to incorrect results. The spectra produced by binary systems (stellar binaries and massive black hole binaries) are presented over the ...

  1. Binary Code Disassembly for Reverse Engineering

    Directory of Open Access Journals (Sweden)

    Marius Popa

    2013-01-01

    Full Text Available The disassembly of binary file is used to restore the software application code in a readable and understandable format for humans. Further, the assembly code file can be used in reverse engineering processes to establish the logical flows of the computer program or its vulnerabilities in real-world running environment. The paper highlights the features of the binary executable files under the x86 architecture and portable format, presents issues of disassembly process of a machine code file and intermediate code, disassembly algorithms which can be applied to a correct and complete reconstruction of the source file written in assembly language, and techniques and tools used in binary code disassembly.

  2. Supermassive Black Hole Binaries as Galactic Blenders

    CERN Document Server

    Kandrup, H E; Terzic, B; Bohn, C L; Kandrup, Henry E.; Sideris, Ioannis V.; Terzic, Balsa; Bohn, Courtlandt L.

    2003-01-01

    This paper focuses on the dynamical implications of close supermassive black hole binaries both as an example of resonant phase mixing and as a potential explanation of inversions and other anomalous features observed in the luminosity profiles of some elliptical galaxies. The presence of a binary comprised of black holes executing nearly periodic orbits leads to the possibility of a broad resonant coupling between the black holes and various stars in the galaxy. This can result in efficient chaotic phase mixing and, in many cases, systematic increases in the energies of stars and their consequent transport towards larger radii. Allowing for the presence of a supermassive black hole binary with plausible parameter values near the center of a spherical, or nearly spherical, galaxy characterised initially by a Nuker density profile enables one to reproduce in considerable detail the central surface brightness distributions of such galaxies as NGC 3706.

  3. CALCULATING THE HABITABLE ZONE OF BINARY STAR SYSTEMS. I. S-TYPE BINARIES

    International Nuclear Information System (INIS)

    We have developed a comprehensive methodology for calculating the boundaries of the habitable zone (HZ) of planet-hosting S-type binary star systems. Our approach is general and takes into account the contribution of both stars to the location and extent of the binary HZ with different stellar spectral types. We have studied how the binary eccentricity and stellar energy distribution affect the extent of the HZ. Results indicate that in binaries where the combination of mass-ratio and orbital eccentricity allows planet formation around a star of the system to proceed successfully, the effect of a less luminous secondary on the location of the primary's HZ is generally negligible. However, when the secondary is more luminous, it can influence the extent of the HZ. We present the details of the derivations of our methodology and discuss its application to the binary HZ around the primary and secondary main-sequence stars of an FF, MM, and FM binary, as well as two known planet-hosting binaries α Cen AB and HD 196886

  4. CALCULATING THE HABITABLE ZONE OF BINARY STAR SYSTEMS. I. S-TYPE BINARIES

    Energy Technology Data Exchange (ETDEWEB)

    Kaltenegger, Lisa [MPIA, Koenigstuhl 17, D-69117 Heidelberg (Germany); Haghighipour, Nader, E-mail: kaltenegger@mpia.de [Institute for Astronomy and NASA Astrobiology Institute, University of Hawaii-Manoa, Honolulu, HI 96822 (United States)

    2013-11-10

    We have developed a comprehensive methodology for calculating the boundaries of the habitable zone (HZ) of planet-hosting S-type binary star systems. Our approach is general and takes into account the contribution of both stars to the location and extent of the binary HZ with different stellar spectral types. We have studied how the binary eccentricity and stellar energy distribution affect the extent of the HZ. Results indicate that in binaries where the combination of mass-ratio and orbital eccentricity allows planet formation around a star of the system to proceed successfully, the effect of a less luminous secondary on the location of the primary's HZ is generally negligible. However, when the secondary is more luminous, it can influence the extent of the HZ. We present the details of the derivations of our methodology and discuss its application to the binary HZ around the primary and secondary main-sequence stars of an FF, MM, and FM binary, as well as two known planet-hosting binaries α Cen AB and HD 196886.

  5. Binary black holes' effects on electromagnetic fields.

    Science.gov (United States)

    Palenzuela, Carlos; Anderson, Matthew; Lehner, Luis; Liebling, Steven L; Neilsen, David

    2009-08-21

    In addition to producing gravitational waves, the dynamics of a binary black hole system could induce emission of electromagnetic radiation by affecting the behavior of plasmas and electromagnetic fields in their vicinity. We here study how the electromagnetic fields are affected by a pair of orbiting black holes through the merger. In particular, we show how the binary's dynamics induce a variability in possible electromagnetically induced emissions as well as a possible enhancement of electromagnetic fields during the late-merge and merger epochs. These time dependent features will likely leave their imprint in processes generating detectable emissions and can be exploited in the detection of electromagnetic counterparts of gravitational waves. PMID:19792706

  6. Spin supplementary conditions for spinning compact binaries

    CERN Document Server

    Mikóczi, Balázs

    2016-01-01

    We consider the different spin supplementary conditions (SSC) for a spinning compact binary with the leading-order spin-orbit (SO) interaction. The Lagrangian of the binary system can be constructed but it is acceleration-dependent in two cases of SSC. We rewrite the generalized Hamiltonian formalism proposed by Ostrogradsky and compute the conservative quantities and the dissipative part of relative motion during the gravitational radiation of each SSCs. We give the orbital elements and observed quantities of the SO dynamics, for instance the energy and the orbital angular momentum losses and waveforms and discuss their SSC dependence.

  7. Quasi periodic oscillations in black hole binaries

    CERN Document Server

    Motta, S E

    2016-01-01

    Fast time variability is the most prominent characteristic of accreting systems and the presence of quasi periodic oscillations (QPOs) is a constant in all accreting systems, from cataclysmic variables to AGNs, passing through black hole and neutron star X-ray binaries and through the enigmatic ultra-luminous X-ray sources. In this paper I will briefly review the current knowledge of QPOs in black hole X-ray binaries, mainly focussing on their observed properties, but also mentioning the most important models that have been proposed to explain the origin of QPOs over the last decades.

  8. Photometric constraints on binary asteroid dynamics

    Science.gov (United States)

    Scheirich, Peter

    2015-08-01

    To date, about 50 binary NEAs, 20 Mars-crossing and 80 small MB asteroids are known. We observe also a population of about 200 unbound asteroid systems (asteroid pairs). I will review the photometric observational data we have for the best observed cases and compare them with theories of binary and paired asteroids evolution.The observed characteristics of asteroid systems suggest their formation by rotational fission of parent rubble-pile asteroids after being spun up by the YORP effect. The angular momentum content of binary asteroids is close to critical. The orientations of satellite orbits of observed binary systems are non-random; the orbital poles concentrate near the obliquities of 0 and 180 degrees, i.e., near the YORP asymptotic states.Recently, a significant excess of retrograde satellite orbits was detected, which is not yet explained characteristic.An evolution of binary system depend heavily on the BYORP effect. If BYORP is contractive, the primary and secondary could end in a tidal-BYORP equilibrium. Observations of mutual events between binary components in at least four apparitions are needed for BYORP to be revealed by detecting a quadratic drift in mean anomaly of the satellite. I will show the observational evidence of single-synchronous binary asteroid with tidally locked satellite (175706 1996 FG3), i.e, with the quadratic drift equal to zero, and binary asteroid with contracting orbit (88710 2001 SL9), with positive value of the quadratic drift (the solution for the quadratic drift is ambiguous so far, with possible values of 5 and 8 deg/yr2).The spin configuration of the satellite play a crucial role in the evolution of the system under the influence of the BYORP effect. I will show that the rotational lightcurves of the satellites show that most of them have small libration amplitudes (up to 20 deg.), with a few interesting exceptions.Acknowledgements: This work has been supported by the Grant Agency of the Czech Republic, Grant P209

  9. Binary Sparse Phase Retrieval via Simulated Annealing

    Directory of Open Access Journals (Sweden)

    Wei Peng

    2016-01-01

    Full Text Available This paper presents the Simulated Annealing Sparse PhAse Recovery (SASPAR algorithm for reconstructing sparse binary signals from their phaseless magnitudes of the Fourier transform. The greedy strategy version is also proposed for a comparison, which is a parameter-free algorithm. Sufficient numeric simulations indicate that our method is quite effective and suggest the binary model is robust. The SASPAR algorithm seems competitive to the existing methods for its efficiency and high recovery rate even with fewer Fourier measurements.

  10. Inducing Risk Neutral Preferences with Binary Lotteries

    DEFF Research Database (Denmark)

    Harrison, Glenn W.; Martínez-Correa, Jimmy; Swarthout, J. Todd

    2013-01-01

    We evaluate the binary lottery procedure for inducing risk neutral behavior. We strip the experimental implementation down to bare bones, taking care to avoid any potentially confounding assumptions about behavior having to be made. In particular, our evaluation does not rely on the assumed...... validity of any strategic equilibrium behavior, or even the customary independence axiom. We show that subjects sampled from our population are generally risk averse when lotteries are defined over monetary outcomes, and that the binary lottery procedure does indeed induce a statistically significant shift...... toward risk neutrality. This striking result generalizes to the case in which subjects make several lottery choices and one is selected for payment....

  11. Toroidal Horizons in Binary Black Hole Mergers

    OpenAIRE

    Bohn, Andy; Kidder, Lawrence E.; Teukolsky, Saul A.

    2016-01-01

    We find the first binary black hole event horizon with a toroidal topology. It had been predicted that generically the event horizons of merging black holes should briefly have a toroidal topology, but such a phase has never been seen prior to this work. In all previous binary black hole simulations, in the coordinate slicing used to evolve the black holes, the topology of the event horizon transitions directly from two spheres during the inspiral to a single sphere as the black holes merge. ...

  12. Binaries in the Hipparcos data: Keep digging

    OpenAIRE

    Pourbaix, D.; Jancart, S.; Jorissen, A.

    2004-01-01

    Among the 120 000 objects in the Hipparcos catalogue, only 235 were fitted with an orbital model. Besides these 235 original astrometric binaries, most Hipparcos entries with a known spectroscopic orbit (extrasolar planet or stellar companion) have now been re-processed, as part of the on-going construction of the 9th Catalogue of Spectroscopic Binary Orbits (SB9, available at http://sb9.astro.ulb.ac.be). The pitfalls and successes of this re-processing are discussed in various contexts, like...

  13. Apsidal motion in eclipsing binary GG Orionis

    Science.gov (United States)

    Yilan, E.; Bulut, I.

    2016-03-01

    The study of apsidal motion in binary stars with eccentric orbit is well known as an important source of information for the stellar internal structure as well as the possibility of verification of general relativity. In this study, the apsidal motion of the eccentric eclipsing binary GG Ori (P = 6.631 days, e = 0.22) has been analyzed using the times of minimum light taken from the literature and databases and the elements of apsidal motion have been computed. The method described by Giménez and García-Pelayo (1983) has been used for the apsidal motion analysis.

  14. Decomposition of Binary Signed-Graphic Matroids

    CERN Document Server

    Pitsoulis, Leonidas

    2010-01-01

    In this paper we employ Tutte's theory of bridges to derive a decomposition theorem for binary matroids arising from signed graphs. The proposed decomposition differs from previous decomposition results on matroids that have appeared in the literature in the sense that it is not based on $k$-sums, but rather on the operation of deletion of a cocircuit. Specifically, it is shown that certain minors resulting from the deletion of a cocircuit of a binary matroid will be graphic matroids apart from exactly one that will be signed-graphic, if and only if the matroid is signed-graphic.

  15. Quantitative spectroscopy of close binary stars

    CERN Document Server

    Pavlovski, K

    2011-01-01

    The method of spectral disentangling has now created the opportunity for studying the chemical composition in previously inaccessible components of binary and multiple stars. This in turn makes it possible to trace their chemical evolution, a vital aspect in understanding the evolution of stellar systems. We review different ways to reconstruct individual spectra from eclipsing and non-eclipsing systems, and then concentrate on some recent applications to detached binaries with high-mass and intermediate-mass stars, and Algol-type mass-transfer systems.

  16. Neutron-Star-Black-Hole Binaries Produced by Binary-Driven Hypernovae.

    Science.gov (United States)

    Fryer, Chris L; Oliveira, F G; Rueda, J A; Ruffini, R

    2015-12-01

    Binary-driven hypernovae (BdHNe) within the induced gravitational collapse paradigm have been introduced to explain energetic (E_{iso}≳10^{52}  erg), long gamma-ray bursts (GRBs) associated with type Ic supernovae (SNe). The progenitor is a tight binary composed of a carbon-oxygen (CO) core and a neutron-star (NS) companion, a subclass of the newly proposed "ultrastripped" binaries. The CO-NS short-period orbit causes the NS to accrete appreciable matter from the SN ejecta when the CO core collapses, ultimately causing it to collapse to a black hole (BH) and producing a GRB. These tight binaries evolve through the SN explosion very differently than compact binaries studied in population synthesis calculations. First, the hypercritical accretion onto the NS companion alters both the mass and the momentum of the binary. Second, because the explosion time scale is on par with the orbital period, the mass ejection cannot be assumed to be instantaneous. This dramatically affects the post-SN fate of the binary. Finally, the bow shock created as the accreting NS plows through the SN ejecta transfers angular momentum, braking the orbit. These systems remain bound even if a large fraction of the binary mass is lost in the explosion (well above the canonical 50% limit), and even large kicks are unlikely to unbind the system. Indeed, BdHNe produce a new family of NS-BH binaries unaccounted for in current population synthesis analyses and, although they may be rare, the fact that nearly 100% remain bound implies that they may play an important role in the compact merger rate, important for gravitational waves that, in turn, can produce a new class of ultrashort GRBs. PMID:26684106

  17. Retrograde binaries of massive black holes in circum-binary accretion discs

    OpenAIRE

    Amaro-Seoane, Pau; Maureira-Fredes, Cristián; Dotti, Massimo; Colpi, Monica

    2016-01-01

    We explore the hardening of a massive black hole binary embedded in a circum-binary gas disc when the binary and the gas are coplanar and the gas is counter-rotating. The secondary black hole, revolving in the direction opposite to the gas, experiences a drag from gas-dynamical friction and from direct accretion of part of it. Using two-dimensional (2D) hydrodynamical grid simulations we investigate the effect of changing the accretion prescriptions on the dynamics of the secondary black hole...

  18. A Survey Design for a Sensitive Binary Variable Correlated with Another Nonsensitive Binary Variable

    OpenAIRE

    Jun-Wu Yu; Guo-Liang Tian; Yang Lu

    2013-01-01

    Tian et al. (2007) introduced a so-called hidden sensitivity model for evaluating the association of two sensitive questions with binary outcomes. However, in practice, we sometimes need to assess the association between one sensitive binary variable (e.g., whether or not a drug user, the number of sex partner being ⩽1 or >1, and so on) and one nonsensitive binary variable (e.g., good or poor health status, with or without cervical cancer, and so on). To address this issue, by sufficiently ut...

  19. Adiabatic Mass Loss Model in Binary Stars

    Science.gov (United States)

    Ge, H. W.

    2012-07-01

    Rapid mass transfer process in the interacting binary systems is very complicated. It relates to two basic problems in the binary star evolution, i.e., the dynamically unstable Roche-lobe overflow and the common envelope evolution. Both of the problems are very important and difficult to be modeled. In this PhD thesis, we focus on the rapid mass loss process of the donor in interacting binary systems. The application to the criterion of dynamically unstable mass transfer and the common envelope evolution are also included. Our results based on the adiabatic mass loss model could be used to improve the binary evolution theory, the binary population synthetic method, and other related aspects. We build up the adiabatic mass loss model. In this model, two approximations are included. The first one is that the energy generation and heat flow through the stellar interior can be neglected, hence the restructuring is adiabatic. The second one is that he stellar interior remains in hydrostatic equilibrium. We model this response by constructing model sequences, beginning with a donor star filling its Roche lobe at an arbitrary point in its evolution, holding its specific entropy and composition profiles fixed. These approximations are validated by the comparison with the time-dependent binary mass transfer calculations and the polytropic model for low mass zero-age main-sequence stars. In the dynamical time scale mass transfer, the adiabatic response of the donor star drives it to expand beyond its Roche lobe, leading to runaway mass transfer and the formation of a common envelope with its companion star. For donor stars with surface convection zones of any significant depth, this runaway condition is encountered early in mass transfer, if at all; but for main sequence stars with radiative envelopes, it may be encountered after a prolonged phase of thermal time scale mass transfer, so-called delayed dynamical instability. We identify the critical binary mass ratio for the

  20. Evolution of Binary Stars in Multiple-Population Globular Clusters

    CERN Document Server

    Hong, Jongsuk; Sollima, Antonio; McMillan, Stephen L W; D'Antona, Franca; D'Ercole, Annibale

    2015-01-01

    The discovery of multiple stellar populations in globular clusters has implications for all the aspects of the study of these stellar systems. In this paper, by means of N-body simulations, we study the evolution of binary stars in multiple-population clusters and explore the implications of the initial differences in the spatial distribution of different stellar populations for the evolution and survival of their binary stars. Our simulations show that initial differences between the spatial distribution of first-generation (FG) and second-generation (SG) stars can leave a fingerprint in the current properties of the binary population. SG binaries are disrupted more efficiently than those of the FG population resulting in a global SG binary fraction smaller than that of the FG. As for surviving binaries, dynamical evolution produces a difference between the SG and the FG binary binding energy distribution with the SG population characterized by a larger fraction of high binding energy (more bound) binaries. ...

  1. Model-independent inference on compact-binary observations

    CERN Document Server

    Mandel, Ilya; Colonna, Andrea; Stevenson, Simon; Tiňo, Peter; Veitch, John

    2016-01-01

    The recent advanced LIGO detections of gravitational waves from merging binary black holes enhance the prospect of exploring binary evolution via gravitational-wave observations of a population of compact-object binaries. In the face of uncertainty about binary formation models, model-independent inference provides an appealing alternative to comparisons between observed and modelled populations. We describe a procedure for clustering in the multi-dimensional parameter space of observations that are subject to significant measurement errors. We apply this procedure to a mock data set of population-synthesis predictions for the masses of merging compact binaries convolved with realistic measurement uncertainties, and demonstrate that we can accurately distinguish subpopulations of binary neutron stars, binary black holes, and mixed black hole -- neutron star binaries.

  2. BINARY ASTEROID ENCOUNTERS WITH TERRESTRIAL PLANETS: TIMESCALES AND EFFECTS

    International Nuclear Information System (INIS)

    Many asteroids that make close encounters with terrestrial planets are in a binary configuration. Here, we calculate the relevant encounter timescales and investigate the effects of encounters on a binary's mutual orbit. We use a combination of analytical and numerical approaches with a wide range of initial conditions. Our test cases include generic binaries with close, moderate, and wide separations, as well as seven well-characterized near-Earth binaries. We find that close approaches (<10 Earth radii) occur for almost all binaries on 1-10 million year timescales. At such distances, our results suggest substantial modifications to a binary's semimajor axis, eccentricity, and inclination, which we quantify. Encounters within 30 Earth radii typically occur on sub-million year timescales and significantly affect the wider binaries. Important processes in the lives of near-Earth binaries, such as tidal and radiative evolution, can be altered or stopped by planetary encounters.

  3. Formation and evolution of X-ray binaries

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    We review recent progress in theoretical understanding of X-ray binaries,which has largely been driven by new observations.We select several topics including formation of compact low-mass X-ray binaries,the evolutionary connection between low-mass X-ray binaries and binary and millisecond radio pulsars,and ultraluminous X-ray sources,to illustrate the interplay between theories and observations.

  4. Binary Solid-Liquid Phase Equilibria

    Science.gov (United States)

    Ellison, Herbert R.

    1978-01-01

    Indicates some of the information that may be obtained from a binary solid-liquid phase equilibria experiment and a method to write a computer program that will plot an ideal phase diagram to which the experimental results may be compared. (Author/CP)

  5. The Environment of Binary Nuetron Star Mergers

    Science.gov (United States)

    Wiggins, Brandon

    2016-04-01

    In addition to detections by LIGO, binary neutron star mergers may be detected via luminous interaction with surrounding interstellar media. Upcoming observations including the VLASS survey may be able to detect such interactions and offer constraints on the binary neutron star merger rate. In this talk, I will present the results of cosmological simulations of a cluster of galaxies followed down to redshift 0. Our calculation includes star formation from which we infer a supernova and binary neutron star production rate. Using pre-existing models of neutron star binaries, we follow the positions of neutron star pairs in the cluster potential throughout cosmic time allowing us to identify regions in which neutron stars merge. We present statistics of many Monte Carlo instances of nuetron star pairs and trajectories allowing us to constrain the approximate fraction of neutron stars merging in dense gas. Our work has implications for R-process enrichment of galaxies in addition to predicting electromagnetic counterparts to gravitational wave detections of neutron star mergers.

  6. The Evolution of Relativistic Binary Progenitor Systems

    CERN Document Server

    Francischelli, G J; Brown, G E

    2001-01-01

    Relativistic binary pulsars, such as B1534+12 and B1913+16 are characterized by having close orbits with a binary separation of ~ 3 R_\\sun. The progenitor of such a system is a neutron star, helium star binary. The helium star, with a strong stellar wind, is able to spin up its compact companion via accretion. The neutron star's magnetic field is then lowered to observed values of about 10^{10} Gauss. As the pulsar lifetime is inversely proportional to its magnetic field, the possibility of observing such a system is, thus, enhanced by this type of evolution. We will show that a nascent (Crab-like) pulsar in such a system can, through accretion-braking torques (i.e. the "propeller effect") and wind-induced spin-up rates, reach equilibrium periods that are close to observed values. Such processes occur within the relatively short helium star lifetimes. Additionally, we find that the final outcome of such evolutionary scenarios depends strongly on initial parameters, particularly the initial binary separation a...

  7. The Binary Pulsar: Gravity Waves Exist.

    Science.gov (United States)

    Will, Clifford

    1987-01-01

    Reviews the history of pulsars generally and the 1974 discovery of the binary pulsar by Joe Taylor and Russell Hulse specifically. Details the data collection and analysis used by Taylor and Hulse. Uses this discussion as support for Albert Einstein's theory of gravitational waves. (CW)

  8. A Binary Teetering on the Edge

    Science.gov (United States)

    Motl, P. M.; D'Souza, M. C. R.; Tohline, J. E.; Frank, J.

    2005-05-01

    We present a fully three-dimensional hydrodynamical simulation of Roche lobe overflow in a binary near the stability boundary. This boundary separates evolutionary branches that correspond to either an accelerating mass transfer rate leading eventually to merger through tidal instability or to a decaying mass transfer rate as the orbit expands. The binary begins with a mass ratio of 0.4 (ratio of donor to accretor mass) and is initially assumed to be rotating synchronously. We treat the stellar components as simple polytropic fluids characterized by a polytropic index, n = 3/2. As the donor overflows its Roche lobe, the mass transfer rate initially accelerates before stabilizing and eventually dropping over a timescale of tens of orbits. We also note that for this particular binary, the accretion stream impacts on the surface of the donor rather than forming an accretion disk. This simulation allows us to measure the efficiency with which the accretion stream spins up the accretor in this "direct impact" scenario and the degree to which angular momentum is transfered back to the binary orbit via the tidal field.

  9. Pedestrian Detection Using Gradient Local Binary Patterns

    Science.gov (United States)

    Jiang, Ning; Xu, Jiu; Goto, Satoshi

    In recent years, local pattern based features have attracted increasing interest in object detection and recognition systems. Local Binary Pattern (LBP) feature is widely used in texture classification and face detection. But the original definition of LBP is not suitable for human detection. In this paper, we propose a novel feature named gradient local binary patterns (GLBP) for human detection. In this feature, original 256 local binary patterns are reduced to 56 patterns. These 56 patterns named uniform patterns are used for generating a 56-bin histogram. And gradient value of each pixel is set as the weight which is always same in LBP based features in histogram calculation to computing the values in 56 bins for histogram. Experiments are performed on INRIA dataset, which shows the proposal GLBP feature is discriminative than histogram of orientated gradient (HOG), Semantic Local Binary Patterns (S-LBP) and histogram of template (HOT). In our experiments, the window size is fixed. That means the performance can be improved by boosting methods. And the computation of GLBP feature is parallel, which make it easy for hardware acceleration. These factors make GLBP feature possible for real-time pedestrian detection.

  10. Binary translation using peephole translation rules

    Science.gov (United States)

    Bansal, Sorav; Aiken, Alex

    2010-05-04

    An efficient binary translator uses peephole translation rules to directly translate executable code from one instruction set to another. In a preferred embodiment, the translation rules are generated using superoptimization techniques that enable the translator to automatically learn translation rules for translating code from the source to target instruction set architecture.

  11. Flip-flopping binary black holes.

    Science.gov (United States)

    Lousto, Carlos O; Healy, James

    2015-04-10

    We study binary spinning black holes to display the long term individual spin dynamics. We perform a full numerical simulation starting at an initial proper separation of d≈25M between equal mass holes and evolve them down to merger for nearly 48 orbits, 3 precession cycles, and half of a flip-flop cycle. The simulation lasts for t=20 000M and displays a total change in the orientation of the spin of one of the black holes from an initial alignment with the orbital angular momentum to a complete antialignment after half of a flip-flop cycle. We compare this evolution with an integration of the 3.5 post-Newtonian equations of motion and spin evolution to show that this process continuously flip flops the spin during the lifetime of the binary until merger. We also provide lower order analytic expressions for the maximum flip-flop angle and frequency. We discuss the effects this dynamics may have on spin growth in accreting binaries and on the observational consequences for galactic and supermassive binary black holes. PMID:25910104

  12. Numerical simulations of compact object binaries

    OpenAIRE

    Pfeiffer, Harald P.

    2012-01-01

    Coalescing compact object binaries consisting of black holes and/or Neutron stars are a prime target for ground-based gravitational wave detectors. This article reviews the status of numerical simulations of these systems, with an emphasis on recent progress.

  13. PARTICLE SEGREGATION IN FLUIDIZED BINARY-MIXTURES

    NARCIS (Netherlands)

    HOFFMANN, AC; JANSSEN, LPBM

    1993-01-01

    The particle segregation in fluidised beds consisting of different types of binary mixtures is shown to be governed by the same particle transport processes. The segregation behaviour of both ''different-density mixtures'' and ''equal-density mixtures'', two types of system which until now largely h

  14. Planetary nebula progenitors that swallow binary systems

    CERN Document Server

    Soker, Noam

    2015-01-01

    I propose that some irregular `messy' planetary nebulae owe their morphologies to triple-stellar evolution where tight binary systems are tidally and frictionally destroyed inside the envelope of asymptotic giant branch (AGB) stars. The tight binary system might breakup with one star leaving the system. In an alternative evolution, one of the stars of the brook-up tight binary system falls toward the AGB envelope with low specific angular momentum, and drowns in the envelope. In a different type of destruction process the drag inside the AGB envelope causes the tight binary system to merge. This releases gravitational energy within the AGB envelope, leading to a very asymmetrical envelope ejection, with an irregular and `messy' planetary nebula as a descendant. The evolution of the triple-stellar system before destruction can be in a full common envelope evolution (CEE) or in a grazing envelope evolution (GEE). Both before and after destruction the system might lunch pairs of opposite jets. One pronounced sig...

  15. Locating Restricted Facilities on Binary Maps

    CERN Document Server

    Andreica, Mugurel Ionut; Andreica, Madalina Ecaterina

    2008-01-01

    In this paper we consider several facility location problems with applications to cost and social welfare optimization, when the area map is encoded as a binary (0,1) mxn matrix. We present algorithmic solutions for all the problems. Some cases are too particular to be used in practical situations, but they are at least a starting point for more generic solutions.

  16. Cassini states for black hole binaries

    Science.gov (United States)

    Correia, Alexandre C. M.

    2016-03-01

    Cassini states correspond to the equilibria of the spin axis of a body when its orbit is perturbed. They were initially described for planetary satellites, but the spin axes of black hole binaries also present this kind of equilibria. In previous works, Cassini states were reported as spin-orbit resonances, but actually the spin of black hole binaries is in circulation and there is no resonant motion. Here we provide a general description of the spin dynamics of black hole binary systems based on a Hamiltonian formalism. In absence of dissipation, the problem is integrable and it is easy to identify all possible trajectories for the spin for a given value of the total angular momentum. As the system collapses due to radiation reaction, the Cassini states are shifted to different positions, which modifies the dynamics around them. This is why the final spin distribution may differ from the initial one. Our method provides a simple way of predicting the distribution of the spin of black hole binaries at the end of the inspiral phase.

  17. Non-binary or genderqueer genders.

    Science.gov (United States)

    Richards, Christina; Bouman, Walter Pierre; Seal, Leighton; Barker, Meg John; Nieder, Timo O; T'Sjoen, Guy

    2016-01-01

    Some people have a gender which is neither male nor female and may identify as both male and female at one time, as different genders at different times, as no gender at all, or dispute the very idea of only two genders. The umbrella terms for such genders are 'genderqueer' or 'non-binary' genders. Such gender identities outside of the binary of female and male are increasingly being recognized in legal, medical and psychological systems and diagnostic classifications in line with the emerging presence and advocacy of these groups of people. Population-based studies show a small percentage--but a sizable proportion in terms of raw numbers--of people who identify as non-binary. While such genders have been extant historically and globally, they remain marginalized, and as such--while not being disorders or pathological in themselves--people with such genders remain at risk of victimization and of minority or marginalization stress as a result of discrimination. This paper therefore reviews the limited literature on this field and considers ways in which (mental) health professionals may assist the people with genderqueer and non-binary gender identities and/or expressions they may see in their practice. Treatment options and associated risks are discussed. PMID:26753630

  18. Eclipsing Binaries with the Kepler Mission

    Science.gov (United States)

    Prsa, Andrej; Kepler Eclipsing Binary Working Group

    2012-05-01

    Kepler has revolutionized the eclipsing binary field by providing us essentially uninterrupted data of unprecedented quality. Out of 160,000 targets, we detected over 2500 eclipsing binaries. These range in orbital periods from as short as 0.3 days, all the way to several years, and encompass stellar types across the H-R diagram. In this talk I will present the collaborative effort of the Kepler Eclipsing Binary Working Group to study and characterize these systems on a statistical level: their distribution in periods, galactic latitude, spectral type, fundamental stellar properties and multiplicity as evidenced by eclipse timing variations. I will further show the gems that have sprung from this sample, which were modeled and interpreted to reveal intrinsically pulsating components, runaway encounters with massive tertiaries, stellar objects that populate the lowest end of the main sequence and circumbinary planets. I will critically review and discuss the causes of data systematics and detrending, and introduce a novel algorithm to classify light curves into morphological types using Locally Linear Embedding. Finally, I will touch on the dark side of eclipsing binaries as the primary cause of false positives in extrasolar planet detections with Kepler.

  19. Spin frequency distributions of binary millisecond pulsars

    NARCIS (Netherlands)

    A. Papitto; D.F. Torres; N. Rea; T.M. Tauris

    2014-01-01

    Rotation-powered millisecond radio pulsars have been spun up to their present spin period by a 108−109 yr long X-ray-bright phase of accretion of matter and angular momentum in a low-to-intermediate mass binary system. Recently, the discovery of transitional pulsars that alternate cyclically between

  20. Short-timescale variability in cataclysmic binaries

    International Nuclear Information System (INIS)

    Rapid variability, including flickering and pulsations, has been detected in cataclysmic binaries at optical and x-ray frequencies. In the case of the novalike variable TT Arietis, simultaneous observations reveal that the x-ray and optical flickering activity is strongly correlated, while short period pulsations are observed that occur at the same frequencies in both wavelength bands

  1. Cassini states for black-hole binaries

    CERN Document Server

    Correia, Alexandre C M

    2016-01-01

    Cassini states correspond to equilibria of the spin axis of a celestial body when its orbit is perturbed. They were initially described for planetary satellites, but the spin axes of black-hole binaries also present this kind of equilibria. In previous works, Cassini states were reported as spin-orbit resonances, but actually the spin of black-hole binaries is in circulation and there is no resonant motion. Here we provide a general description of the spin dynamics of black-hole binary systems based on a Hamiltonian formalism. In absence of dissipation the problem is integrable and it is easy to identify all possible trajectories for the spin for a given value of the total angular momentum. As the system collapses due to radiation reaction, the Cassini states are shifted to different positions, which modifies the dynamics around them. This is why the final spin distribution may differ from the initial one. Our method provides a simple way of predicting the distribution of the spin of black-hole binaries at th...

  2. Supermassive Black Hole Binaries: The Search Continues

    CERN Document Server

    Bogdanovic, Tamara

    2014-01-01

    Gravitationally bound supermassive black hole binaries (SBHBs) are thought to be a natural product of galactic mergers and growth of the large scale structure in the universe. They however remain observationally elusive, thus raising a question about characteristic observational signatures associated with these systems. In this conference proceeding I discuss current theoretical understanding and latest advances and prospects in observational searches for SBHBs.

  3. Binary nucleation of water and sodium chloride

    Czech Academy of Sciences Publication Activity Database

    Němec, Tomáš; Maršík, František; Palmer, A.

    2006-01-01

    Roč. 124, č. 4 (2006), 0445091-0445096. ISSN 0021-9606 R&D Projects: GA ČR(CZ) GA101/05/2536 Institutional research plan: CEZ:AV0Z20760514 Keywords : binary nucleation * sodium chloride * water Subject RIV: BJ - Thermodynamics Impact factor: 3.166, year: 2006

  4. Generating quality tetrahedral meshes from binary volumes

    DEFF Research Database (Denmark)

    Hansen, Mads Fogtmann; Bærentzen, Jakob Andreas; Larsen, Rasmus

    2010-01-01

    use these measures to generate high quality meshes from signed distance maps. This paper also describes an approach for computing (smooth) signed distance maps from binary volumes as volumetric data in many cases originate from segmentation of objects from imaging techniques such as CT, MRI, etc. The...

  5. Binary pulsars as dark-matter probes

    CERN Document Server

    Pani, Paolo

    2015-01-01

    During the motion of a binary pulsar around the galactic center, the pulsar and its companion experience a wind of dark-matter particles that can affect the orbital motion through dynamical friction. We show that this effect produces a characteristic seasonal modulation of the orbit and causes a secular change of the orbital period whose magnitude can be well within the astonishing precision of various binary-pulsar observations. Our analysis is valid for binary systems with orbital period longer than a day. By comparing this effect with pulsar-timing measurements, it is possible to derive model-independent upper bounds on the dark-matter density at different distances $D$ from the galactic center. For example, the precision timing of J1713+0747 imposes $\\rho_{\\rm DM}\\lesssim 10^5\\,{\\rm GeV/cm}^3$ at $D\\approx7\\,{\\rm kpc}$. The detection of a binary pulsar at $D\\lesssim 10\\,{\\rm pc}$ could provide stringent constraints on dark-matter halo profiles and on growth models of the central black hole. The Square Kil...

  6. Testing the Binary Trigger Hypothesis in FUors

    CERN Document Server

    Green, Joel D; Rizzuto, Aaron C; Ireland, Michael J; Dupuy, Trent J; Mann, Andrew W; Kuruwita, Rajika

    2016-01-01

    We present observations of three FU Orionis objects (hereafter, FUors) with nonredundant aperture-mask interferometry (NRM) at 1.59 um and 2.12 um that probe for binary companions on the scale of the protoplanetary disk that feeds their accretion outbursts. We do not identify any companions to V1515 Cyg or HBC 722, but we do resolve a close binary companion to V1057 Cyg that is at the diffraction limit (rho = 58.3 +/- 1.4 mas or 30 +/- 5 AU) and currently much fainter than the outbursting star (delta(K') = 3.34 +/- 0.10 mag). Given the flux excess of the outbursting star, we estimate that the mass of the companion (M ~ 0.25 Msun) is similar to or slightly below that of the FUor itself, and therefore it resembles a typical T Tauri binary system. Our observations only achieve contrast limits of delta(K') ~ 4 mag, and hence we are only sensitive to companions that were near or above the pre-outburst luminosity of the FUors. It remains plausible that FUor outbursts could be tied to the presence of a close binary ...

  7. Constraining Binary Stellar Evolution With Pulsar Timing

    Science.gov (United States)

    Ferdman, Robert D.; Stairs, I. H.; Backer, D. C.; Burgay, M.; Camilo, F.; D'Amico, N.; Demorest, P.; Faulkner, A.; Hobbs, G.; Kramer, M.; Lorimer, D. R.; Lyne, A. G.; Manchester, R.; McLaughlin, M.; Nice, D. J.; Possenti, A.

    2006-06-01

    The Parkes Multibeam Pulsar Survey has yielded a significant number of very interesting binary and millisecond pulsars. Two of these objects are part of an ongoing timing study at the Green Bank Telescope (GBT). PSR J1756-2251 is a double-neutron star (DNS) binary system. It is similar to the original Hulse-Taylor binary pulsar system PSR B1913+16 in its orbital properties, thus providing another important opportunity to test the validity of General Relativity, as well as the evolutionary history of DNS systems through mass measurements. PSR J1802-2124 is part of the relatively new and unstudied "intermediate-mass" class of binary system, which typically have spin periods in the tens of milliseconds, and/or relatively massive (> 0.7 solar masses) white dwarf companions. With our GBT observations, we have detected the Shapiro delay in this system, allowing us to constrain the individual masses of the neutron star and white dwarf companion, and thus the mass-transfer history, in this unusual system.

  8. Performance of binary FSK data transmission systems

    Science.gov (United States)

    Batson, B. H.

    1973-01-01

    Matched-filter detection of binary signals is discussed in terms of the probability of bit error. The equations for the probability of error are derived for coherent phase shift keying, and coherent frequency shift keying (FSK). Suboptimum detection of FSK signals is also discussed for discriminators.

  9. BINARY WAVELET TRANSFORM FOR IMAGE REPRESENTATION

    Directory of Open Access Journals (Sweden)

    ASHOK.M

    2011-08-01

    Full Text Available The MRA (Multi resolution analysis for an image provides specific information localized in space or frequency domain .In this paper an efficient representation of the visual information is presented using Binary wavelet Transform for both color and gray level image. Analytical results shows that this method proves to be more efficient in representing the visual information than the earlier

  10. A semantic constraint on binary determiners

    OpenAIRE

    Zuber, Richard

    2009-01-01

    Abstract A type $${\\langle{1^2, 1}\\rangle}$$ quantifier F is symmetric iff F(X, X)(Y) = F(Y, Y)(X). It is shown that quantifiers denoted by irreducible binary determiners in natural languages are both conservative and symmetric and not only conservative.

  11. Orbits of Ten Visual Binary Stars

    Institute of Scientific and Technical Information of China (English)

    B.Novakovi(c)

    2007-01-01

    We present the orbits of ten visual binary stars:WDS 01015+6922.WDS 01424-0645,WDS 01461+6349,WDS 04374-0951,WDS 04478+5318,WDS 05255-0033,WDS 05491+6248,WDS 06404+4058,WDS 07479-1212,and WDS 18384+0850.We have also determined their masses,dynamical parallaxes and ephemerides.

  12. The Benchmark Eclipsing Binary V530 Ori

    DEFF Research Database (Denmark)

    Torres, Guillermo; Lacy, Claud H. Sandberg; Pavlovski, Kresimir;

    2015-01-01

    We report accurate measurements of the physical properties (mass, radius, temperature) of components of the G+M eclipsing binary V530 On. The M-type secondary shows a larger radius and a cooler temperature than predicted by standard stellar evolution models, as has been found for many other low-m...

  13. Cluster selection in binary nuclear models

    CERN Document Server

    Buck, B; Pérez, S M

    2000-01-01

    We present a simple prescription for selecting the cluster and core in a binary cluster-model description of a nucleus. The prescription reproduces the cluster-core combinations used in earlier successful applications of the model, predicts others, and extends the good agreement previously found with observed B(E2; 2 sup + -> 0 sup +) values of actinide nuclei. Refs. 31 (author)

  14. Merging Compact Binaries in Hierarchical Triple Systems: Resonant Excitation of Binary Eccentricity

    CERN Document Server

    Liu, Bin; Yuan, Ye-Fei

    2015-01-01

    The merging of compact binaries play an important role in astrophysical context. The gravitational waves takes the angular momentum off the merging binary, which makes the orbit of the inner binary shrink. In this work, we study the secular dynamics of merging binary with a small perturber in hierarchical triple systems. From our numerical calculations, we find that the triple system goes through a resonant state between the apsidal precession rates of two orbits during the orbital decay, and the eccentricity of the inner orbit is excited, as well as the corresponding gravita- tional wave frequency. Our numerical results could be understood under the linear approximation of small orbital eccentricities and coplanar configuration. Especially, the resonant condition and the excited eccentricity can be estimated analytically.

  15. Infalling clouds on to supermassive black hole binaries - II. Binary evolution and the final parsec problem

    CERN Document Server

    Goicovic, Felipe G; Cuadra, Jorge; Stasyszyn, Federico

    2016-01-01

    The formation of massive black hole binaries (MBHBs) is an unavoidable outcome of galaxy evolution via successive mergers. However, the mechanism that drives their orbital evolution from parsec separations down to the gravitational wave (GW) dominated regime is poorly understood and their final fate is still unclear. If such binaries are embedded in gas-rich and turbulent environments, as observed in remnants of galaxy mergers, the interaction with gas clumps (such as molecular clouds) may efficiently drive their orbital evolution. Using numerical simulations, we test this hypothesis by studying the dynamical evolution of an equal-mass, circular MBHB accreting infalling molecular clouds. We investigate different orbital configurations, modelling a total of 13 systems to explore different possible pericentre distances and relative inclinations of the cloud-binary encounter. We show that the evolution of the binary orbit is dominated by the exchange of angular momentum through gas accretion during the first sta...

  16. Radio evidence for binary super massive black holes

    Science.gov (United States)

    Ekers, R. D.

    2016-02-01

    I present examples of radio AGN with binary nuclei which provide the direct radio evidence for binary Super Massive Black Holes (SMBH) driving the AGN activity. There is also other evidence for distorted radio morphology and periodic variability which may indicate the presence of a second (inactive) SMBH. Finally I enumerate a number of possible radio tracers for the binary SMBH merger events.

  17. Kepler Eclipsing Binary Stars. VIII. Identification of False Positive Eclipsing Binaries and Re-extraction of New Light Curves

    CERN Document Server

    Abdul-Masih, Michael; Conroy, Kyle; Bloemen, Steven; Boyajian, Tabetha; Doyle, Laurance R; Johnston, Cole; Kostov, Veselin; Latham, David W; Matijevic, Gal; Shporer, Avi; Southworth, John

    2016-01-01

    The Kepler Mission has provided unprecedented, nearly continuous photometric data of $\\sim$200,000 objects in the $\\sim$105 deg$^{2}$ field of view from the beginning of science operations in May of 2009 until the loss of the second reaction wheel in May of 2013. The Kepler Eclipsing Binary Catalog contains information including but not limited to ephemerides, stellar parameters and analytical approximation fits for every known eclipsing binary system in the Kepler Field of View. Using Target Pixel level data collected from Kepler in conjunction with the Kepler Eclipsing Binary Catalog, we identify false positives among eclipsing binaries, i.e. targets that are not eclipsing binaries themselves, but are instead contaminated by eclipsing binary sources nearby on the sky and show eclipsing binary signatures in their light curves. We present methods for identifying these false positives and for extracting new light curves for the true source of the observed binary signal. For each source, we extract three separa...

  18. A decoding method of an n length binary BCH code through (n + 1n length binary cyclic code

    Directory of Open Access Journals (Sweden)

    TARIQ SHAH

    2013-09-01

    Full Text Available For a given binary BCH code Cn of length n = 2 s - 1 generated by a polynomial of degree r there is no binary BCH code of length (n + 1n generated by a generalized polynomial of degree 2r. However, it does exist a binary cyclic code C (n+1n of length (n + 1n such that the binary BCH code Cn is embedded in C (n+1n . Accordingly a high code rate is attained through a binary cyclic code C (n+1n for a binary BCH code Cn . Furthermore, an algorithm proposed facilitates in a decoding of a binary BCH code Cn through the decoding of a binary cyclic code C (n+1n , while the codes Cn and C (n+1n have the same minimum hamming distance.

  19. Binary Disk interaction II: Gap-Opening criteria for unequal mass binaries

    CERN Document Server

    del Valle, Luciano

    2013-01-01

    We study the interaction between an unequal mass binary with an isothermal circumbinary disk, motivated by the theoretical and observational evidence that after a major merger of gas-rich galaxies, a massive gaseous disk with a SMBH binary will be formed in the nuclear region. We focus on the gravitational torques that the binary exerts onto the disk and how these torques can drive the formation of a gap in the disk. This exchange of angular momentum between the binary and the disk is mainly driven by the gravitational interaction between the binary and a strong non-axisymmetric density perturbation that is produced in the disk, as response to the presence of the binary. Using SPH numerical simulations we tested two gap-opening criterion, one that assumes that the geometry of the density perturbation is an ellipsoid/thick-spirals and another that assumes a geometry of flat-spirals for the density perturbation. We find that the flat-spirals gap opening criterion successfully predicts which simulations will hav...

  20. Binaries migrating in a gaseous disk: Where are the Galactic center binaries?

    CERN Document Server

    Baruteau, C; Lin, D N C

    2010-01-01

    The massive stars in the Galactic center inner arcsecond share analogous properties with the so-called Hot Jupiters. Most of these young stars have highly eccentric orbits, and were probably not formed in-situ. It has been proposed that these stars acquired their current orbits from the tidal disruption of compact massive binaries scattered toward the proximity of the central supermassive black hole. Assuming a binary star formed in a thin gaseous disk beyond 0.1 pc from the central object, we investigate the relevance of disk-satellite interactions to harden the binding energy of the binary, and to drive its inward migration. A massive, equal-mass binary star is found to become more tightly wound as it migrates inwards toward the central black hole. The migration timescale is very similar to that of a single-star satellite of the same mass. The binary's hardening is caused by the formation of spiral tails lagging the stars inside the binary's Hill radius. We show that the hardening timescale is mostly determ...

  1. White-light Flares on Close Binaries Observed with Kepler

    Science.gov (United States)

    Gao, Qing; Xin, Yu; Liu, Ji-Feng; Zhang, Xiao-Bin; Gao, Shuang

    2016-06-01

    Based on Kepler data, we present the results of a search for white light flares on 1049 close binaries. We identify 234 flare binaries, of which 6818 flares are detected. We compare the flare-binary fraction in different binary morphologies (“detachedness”). The result shows that the fractions in over-contact and ellipsoidal binaries are approximately 10%–20% lower than those in detached and semi-detached systems. We calculate the binary flare activity level (AL) of all the flare binaries, and discuss its variations along the orbital period (P orb) and rotation period (P rot, calculated for only detached binaries). We find that the AL increases with decreasing P orb or P rot, up to the critical values at P orb ∼ 3 days or P rot ∼ 1.5 days, and thereafter the AL starts decreasing no matter how fast the stars rotate. We examine the flaring rate as a function of orbital phase in two eclipsing binaries on which a large number of flares are detected. It appears that there is no correlation between flaring rate and orbital phase in these two binaries. In contrast, when we examine the function with 203 flares on 20 non-eclipse ellipsoidal binaries, bimodal distribution of amplitude-weighted flare numbers shows up at orbital phases 0.25 and 0.75. Such variation could be larger than what is expected from the cross section modification.

  2. Detectability of Gravitational Waves from High-Redshift Binaries.

    Science.gov (United States)

    Rosado, Pablo A; Lasky, Paul D; Thrane, Eric; Zhu, Xingjiang; Mandel, Ilya; Sesana, Alberto

    2016-03-11

    Recent nondetection of gravitational-wave backgrounds from pulsar timing arrays casts further uncertainty on the evolution of supermassive black hole binaries. We study the capabilities of current gravitational-wave observatories to detect individual binaries and demonstrate that, contrary to conventional wisdom, some are, in principle, detectable throughout the Universe. In particular, a binary with rest-frame mass ≳10^{10}M_{⊙} can be detected by current timing arrays at arbitrarily high redshifts. The same claim will apply for less massive binaries with more sensitive future arrays. As a consequence, future searches for nanohertz gravitational waves could be expanded to target evolving high-redshift binaries. We calculate the maximum distance at which binaries can be observed with pulsar timing arrays and other detectors, properly accounting for redshift and using realistic binary waveforms. PMID:27015470

  3. Twin Binaries: Studies of Stability, Mass Transfer, and Coalescence

    CERN Document Server

    Lombardi, James C; Dooley, Katherine L; Gearity, Kyle; Kalogera, Vassiliki; Rasio, Frederic A

    2010-01-01

    Motivated by suggestions that binaries with almost equal-mass components ("twins") play an important role in the formation of double neutron stars and may be rather abundant among binaries, we study the stability of synchronized close and contact binaries with identical components in circular orbits. In particular, we investigate the dependency of the innermost stable circular orbit on the core mass, and we study the coalescence of the binary that occurs at smaller separations. For twin binaries composed of convective main-sequence stars, subgiants, or giants with low mass cores (M_c ~0.15M), we find that stable contact configurations exist at all separations down to the Roche limit, when mass shedding through the outer Lagrangian points triggers a coalescence of the envelopes and leaves the cores orbiting in a central tight binary. We discuss the implications of our results to the formation of binary neutron stars.

  4. Template Mode Hierarchies for Binary Black Hole Mergers

    CERN Document Server

    Healy, James; Pekowsky, Larne; Shoemaker, Deirdre

    2013-01-01

    Matched filtering is a popular data analysis framework used to search for gravitational wave signals emitted by compact object binaries. The templates used in matched filtering searches are constructed predominantly from the quadrupolar mode because this mode is the energetically most dominant channel. However, for highly precessing binaries or binaries with moderately large mass ratios, significant power is also carried by higher-order modes. We investigate how the inclusion of higher modes in the templates increases the prospects for detecting gravitational waves. Specifically, we use numerical relativity waveforms from the late inspiral and coalescence of binary black holes to identify mode hierarchies that cover the sky of binary orientations. We show that the ordering in these hierarchies depends on the characteristics of the binary system and the mode strengths. Our study demonstrates that detecting moderately high precessing or unequal mass binaries requires the inclusion of higher modes in the templat...

  5. Fluorescent Ribozyme and Deoxyribozyme Sensors

    Science.gov (United States)

    Chiuman, William; Li, Yingfu

    The development of allosteric nucleic acid enzymes (NAEs) has made NAEs very attractive for a wide variety of biotechnological applications, including biosensing, diagnostics, drug screening, and molecular computation. Although NAEs alone might have limited values for analytical application due to the rather small scope of their substrates and cofactors, modular characteristics of aptamers and NAEs permit the easy design of combined sensors where the aptamer acts as the molecular recognition element (MRE) and the NAE functions as a reporter. To facilitate the exploitation of NAEs for biosensing applications, fluorescence methods have been increasingly explored as better alternatives to radioisotope-based detection techniques. In this chapter, we first survey the strategies that have been employed to graft fluorescence-signaling moieties onto NAEs. We then review our experimental efforts in creating a group of fluorescence-signaling and RNA-cleaving deoxyribozymes (DNAzymes) intended for the design of fluorescent sensors. Last, we discuss the diverse engineering approaches that can transmit the binding status of an aptamer to the activation or repression of catalytic activity in fluorescent NAE sensors.

  6. Performance analysis and binary working fluid selection of combined flash-binary geothermal cycle

    International Nuclear Information System (INIS)

    Performance of the combined flash-binary geothermal power cycle for geofluid temperatures between 150 and 250 °C is studied. A thermodynamic model is developed, and the suitable binary working fluids for different geofluid temperatures are identified from a list of thirty working fluid candidates, consisting environmental friendly refrigerants and hydrocarbons. The overall system exergy destruction and Vapor Expansion Ratio across the binary cycle turbine are selected as key performance indicators. The results show that for low-temperature heat sources using refrigerants as binary working fluids result in higher overall cycle efficiency and for medium and high-temperature resources, hydrocarbons are more suitable. For combined flash-binary cycle, secondary working fluids; R-152a, Butane and Cis-butane show the best performances at geofluid temperatures 150, 200 and 250 °C respectively. The overall second law efficiency is calculated as high as 0.48, 0.55 and 0.58 for geofluid temperatures equal 150, 200 and 250 °C respectively. The flash separator pressure found to has important effects on cycle operation and performance. Separator pressure dictates the work production share of steam and binary parts of the system. And there is an optimal separator pressure at which overall exergy destruction of the cycle achieves its minimum value. - Highlights: • Performance of the combined flash-binary geothermal cycle is investigated. • Thirty different fluids are screened to find the most suitable ORC working fluid. • Optimum cycle operation conditions presented for geofluids between 150 °C and 250 °C. • Refrigerants are more suitable for the ORC at geothermal sources temperature ≤200 °C. • Hydrocarbons are more suitable for the ORC at geothermal sources temperature >200 °C

  7. Phenomenological gravitational waveforms from spinning coalescing binaries

    CERN Document Server

    Sturani, R; Cadonati, L; Guidi, G M; Healy, J; Shoemaker, D; Vicere', A

    2010-01-01

    An accurate knowledge of the coalescing binary gravitational waveform is crucial for match filtering techniques, which are currently used in the observational searches performed by the LIGO-Virgo collaboration. Following an earlier paper by the same authors we expose the construction of analytical phenomenological waveforms describing the signal sourced by generically spinning binary systems. The gap between the initial inspiral part of the waveform, described by spin-Taylor approximants, and its final ring-down part, described by damped exponentials, is bridged by a phenomenological phase calibrated by comparison with the dominant spherical harmonic mode of a set of waveforms including both numerical and phenomenological waveforms of a different type. All waveforms considered describe equal mass systems with dimension-less spin magnitudes equal to 0.6. The noise-weighted overlap integral between numerical and phenomenological waveforms ranges between 0.93 and 0.98 for a wide span of mass values.

  8. Spin-Spin Coupling in Asteroidal Binaries

    Science.gov (United States)

    Batygin, Konstantin; Morbidelli, Alessandro

    2015-11-01

    Gravitationally bound binaries constitute a substantial fraction of the small body population of the solar system, and characterization of their rotational states is instrumental to understanding their formation and dynamical evolution. Unlike planets, numerous small bodies can maintain a perpetual aspheroidal shape, giving rise to a richer array of non-trivial gravitational dynamics. In this work, we explore the rotational evolution of triaxial satellites that orbit permanently deformed central objects, with specific emphasis on quadrupole-quadrupole interactions. Our analysis shows that in addition to conventional spin-orbit resonances, both prograde and retrograde spin-spin resonances naturally arise for closely orbiting, highly deformed bodies. Application of our results to the illustrative examples of (87) Sylvia and (216) Kleopatra multi-asteroid systems implies capture probabilities slightly below ~10% for leading-order spin-spin resonances. Cumulatively, our results suggest that spin-spin coupling may be consequential for highly elongated, tightly orbiting binary objects.

  9. BISC: binary subcomplexes in proteins database.

    Science.gov (United States)

    Juettemann, Thomas; Gerloff, Dietlind L

    2011-01-01

    Binary subcomplexes in proteins database (BISC) is a new protein-protein interaction (PPI) database linking up the two communities most active in their characterization: structural biology and functional genomics researchers. The BISC resource offers users (i) a structural perspective and related information about binary subcomplexes (i.e. physical direct interactions between proteins) that are either structurally characterized or modellable entries in the main functional genomics PPI databases BioGRID, IntAct and HPRD; (ii) selected web services to further investigate the validity of postulated PPI by inspection of their hypothetical modelled interfaces. Among other uses we envision that this resource can help identify possible false positive PPI in current database records. BISC is freely available at http://bisc.cse.ucsc.edu. PMID:21081561

  10. Error detection by binary sparse matrices

    International Nuclear Information System (INIS)

    Error detection is a crucial process in an information transmission protocol like TCP for attaining reliable communications over unreliable channels. The most important performance measure for an error detection scheme is its undetected error probability, which is the probability corresponding to the event such that an erroneous received word passes the detection test. The paper reviews the author's results on an analysis of the undetected error probability of ensembles of m x n binary matrices. The ensemble of binary sparse matrices called the Bernoulli ensemble whose members are considered as matrices generated from i.i.d. Bernoulli source is mainly discussed here. The error exponent of the average undetected error probability and closed form expressions for the variance of the undetected error probability will be presented.

  11. Hamiltonian Hydrodynamics and Irrotational Binary Inspiral

    CERN Document Server

    Markakis, Charalampos M

    2014-01-01

    Gravitational waves from neutron-star and black-hole binaries carry valuable information on their physical properties and probe physics inaccessible to the laboratory. Although development of black-hole gravitational-wave templates in the past decade has been revolutionary, the corresponding work for double neutron-star systems has lagged. Neutron stars can be well-modelled as simple barotropic fluids during the part of binary inspiral most relevant to gravitational wave astronomy, but the crucial geometric and mathematical consequences of this simplification have remained computationally unexploited. In particular, Carter and Lichnerowicz have described barotropic fluid motion via classical variational principles as conformally geodesic. Moreover, Kelvin's circulation theorem implies that initially irrotational flows remain irrotational. Applied to numerical relativity, these concepts lead to novel Hamiltonian or Hamilton-Jacobi schemes for evolving relativistic fluid flows. Hamiltonian methods can conserve ...

  12. Processes assessment in binary mixture plant

    Directory of Open Access Journals (Sweden)

    N. Shankar Ganesh, T. Srinivas

    2013-01-01

    Full Text Available Binary fluid system has an efficient system of heat recovery compared to a single fluid system due to a better temperature match between hot and cold fluids. There are many applications with binary fluid system i.e. Kalina power generation, vapor absorption refrigeration, combined power and cooling etc. Due to involvement of three properties (pressure, temperature and concentration in the processes evaluation, the solution is complicated compared to a pure substance. The current work simplifies this complex nature of solution and analyzes the basic processes to understand the processes behavior in power generation as well as cooling plants. Kalina power plant consists of regenerator, heat recovery vapor generator, condenser, mixture, separator, turbine, pump and throttling device. In addition to some of these components, the cooling plant consists of absorber which is similar in operation of condenser. The amount of vapor at the separator decreases with an increase in its pressure and temperature.

  13. Binary hidden Markov models and varieties

    CERN Document Server

    Critch, Andrew J

    2012-01-01

    The technological applications of hidden Markov models have been extremely diverse and successful, including natural language processing, gesture recognition, gene sequencing, and Kalman filtering of physical measurements. HMMs are highly non-linear statistical models, and just as linear models are amenable to linear algebraic techniques, non-linear models are amenable to commutative algebra and algebraic geometry. This paper examines closely those HMMs in which all the random variables, called nodes, are binary. Its main contributions are (1) minimal defining equations for the 4-node model, comprising 21 quadrics and 29 cubics, which were computed using Gr\\"obner bases in the cumulant coordinates of Sturmfels and Zwiernik, and (2) a birational parametrization for every binary HMM, with an explicit inverse for recovering the hidden parameters in terms of observables. The new model parameters in (2) are hence rationally identifiable in the sense of Sullivant, Garcia-Puente, and Spielvogel, and each model's Zar...

  14. Binary Minor Planets V9.0

    Science.gov (United States)

    Johnston, W. R.

    2016-07-01

    The data set lists orbital and physical properties for well-observed or suspected binary/multiple minor planets including the Pluto system, compiled from the published literature as inspired by Richardson and Walsh (2006) and similar reviews (Merline et al., 2003; Noll, 2006; Pravec et al., 2006; Pravec and Harris, 2007; Descamps and Marchis, 2008; Noll et al., 2008; Walsh, 2009). In total 297 companions in 282 systems are included. Data are presented in three tables: one for orbital and physical properties; one for companion designations, discovery information, and reference codes for data values; and one giving full references for each reference code. This data set is complete for binary/multiple components reported through 31 March 2016.

  15. Modulated Binary-Ternary Dual Semiconductor Heterostructures.

    Science.gov (United States)

    Prusty, Gyanaranjan; Guria, Amit K; Mondal, Indranil; Dutta, Anirban; Pal, Ujjwal; Pradhan, Narayan

    2016-02-18

    A generic modular synthetic strategy for the fabrication of a series of binary-ternary group II-VI and group I-III-VI coupled semiconductor nano-heterostructures is reported. Using Ag2 Se nanocrystals first as a catalyst and then as sacrificial seeds, four dual semiconductor heterostructures were designed with similar shapes: CdSe-AgInSe2 , CdSe-AgGaSe2 , ZnSe-AgInSe2 , and ZnSe-AgGaSe2 . Among these, dispersive type-II heterostructures are further explored for photocatalytic hydrogen evolution from water and these are observed to be superior catalysts than the binary or ternary semi-conductors. Details of the chemistry of this modular synthesis have been studied and the photophysical processes involved in catalysis are investigated. PMID:26800297

  16. Parallel Matrix Factorization for Binary Response

    CERN Document Server

    Khanna, Rajiv; Agarwal, Deepak; Chen, Beechung

    2012-01-01

    Predicting user affinity to items is an important problem in applications like content optimization, computational advertising, and many more. While bilinear random effect models (matrix factorization) provide state-of-the-art performance when minimizing RMSE through a Gaussian response model on explicit ratings data, applying it to imbalanced binary response data presents additional challenges that we carefully study in this paper. Data in many applications usually consist of users' implicit response that are often binary -- clicking an item or not; the goal is to predict click rates, which is often combined with other measures to calculate utilities to rank items at runtime of the recommender systems. Because of the implicit nature, such data are usually much larger than explicit rating data and often have an imbalanced distribution with a small fraction of click events, making accurate click rate prediction difficult. In this paper, we address two problems. First, we show previous techniques to estimate bi...

  17. Superconducting State Parameters of Binary Superconductors

    Directory of Open Access Journals (Sweden)

    Aditya M. Vora

    2012-05-01

    Full Text Available A well known pseudopotential is used to investigate the superconducting state parameters viz. electron-phonon coupling strength , Coulomb pseudopotential *, transition temperature ТС, isotope effect exponent  and effective interaction strength N0V for the AgxZn1 – x and AgxAl1 – x binary superconductors theoretically for the first time. We have incorporated here five different types of the local field correction functions to show the effect of exchange and correlation on the aforesaid properties. Very strong influence of the various exchange and correlation functions is concluded from the present study. The comparison with other such experimental values is encouraging, which confirms the applicability of the model potential in explaining the superconducting state parameters of binary mixture.

  18. Sequential binary decay of highly excited nuclei

    International Nuclear Information System (INIS)

    The decay of highly excited nuclei is described as a sequence of binary processes involving emission of fragments in their ground, excited-bound and unbound states. Primary together with secondary decay products lead to the final mass distributions. Asymmetric mass splittings involving nucleon emission up to symmetric binary ones are treated according to a generalized Weisskopf evaporation formalism. This procedure is implemented in the Monte-Carlo multi-step statistical model code MECO (Multisequential Evaporation COde). We examine the evolution of the calculated final mass distributions in the decay of a light compound nucleus, as the initial excitation energy increases towards the limits of complete dissociation. Comparisons are made with the predictions of the transition-stage theory, as well as a consistent Weisskopf treatment in which the decay process is described by rate equations for the generation of different fragment species. (author)

  19. Simulation of nuclei morphologies for binary alloy

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    We study the critical nuclei morphologies of a binary alloy by the string method. The dynamic equation of the string, connecting the metastable phase (liquid) and stable phase (solid), is governed by Helmholtz free energy for the binary alloy system at a given temperature. The stationary string through the critical nucleus (saddle point) is obtained if the relaxation time of the string is su?ciently large. The critical nucleus radius and energy barrier to nucleation of a pure alloy with isotropic interface energy in two and three dimensions are calculated, which are consistent with the classical nucleation theory. The critical nuclei morphologies are sensitive to the anisotropy strength of interface energy and interface thickness of alloy in two and three dimensions. The critical nucleus and energy barrier to nucleation become smaller if the anisotropy strength of the interface energy is increased, which means that it is much easier to form a stable nucleus if the anisotropy of the interface energy is considered.

  20. Binary Brayton cycle with two isothermal processes

    International Nuclear Information System (INIS)

    Highlights: • This paper presents binary Brayton cycle with two isothermal processes. • Different parameters affecting the cycle performance have been studied. • The present cycle is a promising cycle for future power generation. - Abstract: The literature introduced isothermal concept and binary Brayton cycle as two promising methods used to enhance the performance of the gas turbine. Consequently, this work presents a cycle based on the blending of the two methods. This cycle is composed of gas turbine topping cycle with isothermal combustion and air turbine bottoming cycle with isothermal heating. Different parameters affecting the cycle performance have been studied. Simulations demonstrate that the present cycle achieves drastic enhancement in performance. The cycle merits justify its potential utilization for future power generation

  1. Toroidal Horizons in Binary Black Hole Mergers

    CERN Document Server

    Bohn, Andy; Teukolsky, Saul A

    2016-01-01

    We find the first binary black hole event horizon with a toroidal topology. It had been predicted that generically the event horizons of merging black holes should briefly have a toroidal topology, but such a phase has never been seen prior to this work. In all previous binary black hole simulations, in the coordinate slicing used to evolve the black holes, the topology of the event horizon transitions directly from two spheres during the inspiral to a single sphere as the black holes merge. We present a coordinate transformation to a foliation of spacelike hypersurfaces that "cut a hole" through the event horizon surface, resulting in a toroidal event horizon. A torus could potentially provide a mechanism for violating topological censorship. However, these toroidal event horizons satisfy topological censorship by construction, because we can always trivially apply the inverse coordinate transformation to remove the topological feature.

  2. A Binary Representation of the Genetic Code

    CERN Document Server

    Nemzer, Louis R

    2016-01-01

    This article introduces a novel binary representation of the canonical genetic code, in which each of the four mRNA nucleotide bases is assigned a unique 2-bit identifier. These designations have a physiological meaning derived from the molecular structures of, and relationships between, the bases. In this scheme, the 64 possible triplet codons are each indexed by a 6-bit label. The order of the bits reflects the hierarchical organization manifested by the DNA replication/repair and tRNA translation systems. Transition and transversion mutations are naturally expressed as basic binary operations, and the severity of the different types is analyzed. Using a principal component analysis, it is shown that physicochemical properties of amino acids related to protein folding also correlate with particular bit positions of their respective labels. Thus, the likelihood for a particular point mutation to be conservative, and therefore less likely to cause a change in protein functionality, can be estimated.

  3. Binary Fingerprints at Fluctuation-Enhanced Sensing

    CERN Document Server

    Chang, Hung-Chih; King, Maria D; Kwan, Chiman

    2009-01-01

    We developed a simple way to generate binary patterns based on spectral slopes in different frequency ranges at fluctuation-enhanced sensing. Such patterns can be considered as binary "fingerprints" of odors. The method has experimentally been demonstrated with a commercial semiconducting metal oxide (Taguchi) sensor exposed to bacterial odors (Escherichia coli and Anthrax-surrogate Bacillus subtilis) and processing their stochastic signals. With a single Taguchi sensor, the situations of empty chamber, tryptic soy agar (TSA) medium, or TSA with bacteria could be distinguished with 100% reproducibility. The bacterium numbers were in the range of 25 thousands to 1 million. To illustrate the relevance for ultra-low power consumption, we show that this new type of signal processing and pattern recognition task can be implemented by a simple analog circuitry and a few logic gates with total power consumption in the microWatts range.

  4. Dynamics and Habitability in Binary Star Systems

    CERN Document Server

    Eggl, Siegfried; Pilat-Lohinger, Elke

    2014-01-01

    Determining planetary habitability is a complex matter, as the interplay between a planet's physical and atmospheric properties with stellar insolation has to be studied in a self consistent manner. Standardized atmospheric models for Earth-like planets exist and are commonly accepted as a reference for estimates of Habitable Zones. In order to define Habitable Zone boundaries, circular orbital configurations around main sequence stars are generally assumed. In gravitationally interacting multibody systems, such as double stars, however, planetary orbits are forcibly becoming non circular with time. Especially in binary star systems even relatively small changes in a planet's orbit can have a large impact on habitability. Hence, we argue that a minimum model for calculating Habitable Zones in binary star systems has to include dynamical interactions.

  5. Buffer Overflow Detection on Binary Code

    Institute of Scientific and Technical Information of China (English)

    ZHENG Yan-fei; LI Hui; CHEN Ke-fei

    2006-01-01

    Most solutions for detecting buffer overflow are based on source code. But the requirement for source code is not always practical especially for business software. A new approach was presented to detect statically the potential buffer overflow vulnerabilities in the binary code of software. The binary code was translated into assembly code without the lose of the information of string operation functions. The feature code abstract graph was constructed to generate more accurate constraint statements, and analyze the assembly code using the method of integer range constraint. After getting the elementary report on suspicious code where buffer overflows possibly happen, the control flow sensitive analysis using program dependence graph was done to decrease the rate of false positive. A prototype was implemented which demonstrates the feasibility and efficiency of the new approach.

  6. Construction of generalized binary Bent sequences

    Institute of Scientific and Technical Information of China (English)

    KE Pin-hui; CHANG Zu-ling; WEN Qiao-yan

    2006-01-01

    Bent functions in trace forms play an important role in the constructions of generalized binary Bent sequences.Trace representation of some degree two Bent functions are presented in this paper.A sufficient and necessary condition is derived to determine whether the sum of the combinations of Gold functions,tr1n(x2'+1),1≤I≤n-1,over finite fields F2n (n be even) in addition to another term tr1n/2(x2n/2+1) is a Bent function.Similar to the result presented by Khoo et al.,the condition can be verified by polynominal greatest common divisor (GCD) computation.A similar result also holds in the case Fpn (n be even,p be odd prime).Using the constructed Bent functions and Niho type Bent functions given by Dobbertin et al.,many new generalized binary Bent sequences are obtained.

  7. Biología reproductiva de la cornuda común Sphyrna lewini Griffith & Smith (Sphyrnidae en el Golfo de California Reproductive Biology of the Scalloped Hammerhead Shark Sphyrna lewini Griffith & Smith (Sphyrnidae in the Gulf of California

    Directory of Open Access Journals (Sweden)

    Ana María Torres-Huerta

    2008-12-01

    Full Text Available Durante el período de 1994 a 1996 se obtuvieron datos de longitud total (LT, peso total, sexo y madurez gonádica de 1 693 individuos de Sphyrna lewini, capturados en ocho campos pesqueros de tiburón en el Golfo de California. En las hembras la longitud total tuvo un intervalo de 41 a 363 cm, mientras que en los machos fue de 43 a 290 cm. La proporción de sexos en embriones, neonatos y juveniles fue de 1:1, no así en adultos donde las proporciones variaron en las localidades de muestreo. Los machos y hembras alcanzaron la talla de primera madurez a los 170 y 207 cm de LT, respectivamente. El período de gestación tiene una duración aproximada de 11 meses y la fecundidad máxima fue de 32 embriones por hembra. El nacimiento de las crías ocurre en junio, con una talla entre 41 y 53 cm de LT. La presencia de neonatos y hembras grávidas durante el verano en la región central y sureste del Golfo de California sugiere que éstas son dos importantes áreas de nacimiento para la cornuda común en el Golfo de California. Mientras que, la costa este del Golfo de California y la Bahía de La Paz funcionan como área de crianza para los juveniles.Reproductive biology of scalloped hammerhead shark Sphyrna lewini, was investigated in eight shark fishing camps in the Gulf of California (Mexico from 1994 to 1996. A total of 1 693 specimens were determined total length (TL, total weight, sex and the gonadal maturity. The total length range was from 41 to 363 cm for females and from 43 to 290 cm for males. The sex proportion of embryos, neonates and juveniles in most of the cases was 1:1; however, this was not the case for adults, where the proportions varied at the sampling sites. Sexual maturity was reached at 170 cm for males and 207 cm TL for females. The gestation period was 11 months and the highest fecundity was 32 embryos. The June births were between 41 and 53 cm TL. The presence of neonates, gravid females, and small juveniles in the east coast

  8. Kepler Eclipsing Binary Stars. V. Identification of 31 Eclipsing Binaries in the K2 Engineering Data-set

    CERN Document Server

    Conroy, Kyle E; Stassun, Keivan G; Bloemen, Steven; Parvizi, Mahmoud; Quarles, Billy; Boyajian, Tabetha; Barclay, Thomas; Shporer, Avi; Latham, David W; Abdul-Masih, Michael

    2014-01-01

    Over 2500 eclipsing binaries were identified and characterized from the ultra-precise photometric data provided by the Kepler space telescope. Kepler is now beginning its second mission, K2, which is proving to again provide ultra-precise photometry for a large sample of eclipsing binary stars. In the 1951 light curves covering 12 days in the K2 engineering data-set, we have identified and determined the ephemerides for 31 eclipsing binaries that demonstrate the capabilities for eclipsing binary science in the upcoming campaigns in K2. Of those, 20 are new discoveries. We describe both manual and automated approaches to harvesting the complete set of eclipsing binaries in the K2 data, provide identifications and details for the full set of eclipsing binaries present in the engineering data-set, and discuss the prospects for application of eclipsing binary searches in the K2 mission.

  9. Binary pulsar - a test for general relativity

    International Nuclear Information System (INIS)

    The binary system of PSR1913 + 16 contains the pulsar and an, as yet unknown, companion. If this star is a compact object too, then the data can be interpreted in terms of general relativistic effects. This leads to the conclusion that the decay of the orbit must be due to the emission of gravitational waves. The nature of the unseen companion is discussed in detail

  10. Gravitational waves from binary black holes

    Indian Academy of Sciences (India)

    Bala R Iyer

    2011-07-01

    It is almost a century since Einstein predicted the existence of gravitational waves as one of the consequences of his general theory of relativity. A brief historical overview including Chandrasekhar’s contribution to the subject is first presented. The current status of the experimental search for gravitational waves and the attendant theoretical insights into the two-body problem in general relativity arising from computations of gravitational waves from binary black holes are then broadly reviewed.

  11. Gravity Waves, Chaos, and Spinning Compact Binaries

    OpenAIRE

    Levin, Janna

    1999-01-01

    Spinning compact binaries are shown to be chaotic in the Post-Newtonian expansion of the two body system. Chaos by definition is the extreme sensitivity to initial conditions and a consequent inability to predict the outcome of the evolution. As a result, the spinning pair will have unpredictable gravitational waveforms during coalescence. This poses a challenge to future gravity wave observatories which rely on a match between the data and a theoretical template.

  12. Modeling Flows Around Merging Black Hole Binaries

    OpenAIRE

    van Meter, James R.; Wise, John H.; Miller, M. Coleman; Reynolds, Christopher S.; Centrella, Joan M.; Baker, John G.; Boggs, William D.; Kelly, Bernard J.; McWilliams, Sean T.

    2009-01-01

    Coalescing massive black hole binaries are produced by the mergers of galaxies. The final stages of the black hole coalescence produce strong gravitational radiation that can be detected by the space-borne LISA. In cases where the black hole merger takes place in the presence of gas and magnetic fields, various types of electromagnetic signals may also be produced. Modeling such electromagnetic counterparts of the final merger requires evolving the behavior of both gas and fields in the stron...

  13. Minimum degree and density of binary sequences

    DEFF Research Database (Denmark)

    Brandt, Stephan; Müttel, J.; Rautenbach, D.;

    2010-01-01

    For d,k∈N with k ≤ 2d, let g(d,k) denote the infimum density of binary sequences (x)∈{0,1} which satisfy the minimum degree condition σ(x+) ≥ k for all i∈Z with xi=1. We reduce the problem of computing g(d,k) to a combinatorial problem related to the generalized k-girth of a graph G which is...

  14. Reconciliation with Non-Binary Species Trees

    OpenAIRE

    Vernot, Benjamin; Stolzer, Maureen; Goldman, Aiton; Durand, Dannie

    2008-01-01

    Reconciliation extracts information from the topological incongruence between gene and species trees to infer duplications and losses in the history of a gene family. The inferred duplication-loss histories provide valuable information for a broad range of biological applications, including ortholog identification, estimating gene duplication times, and rooting and correcting gene trees. While reconciliation for binary trees is a tractable and well studied problem, there are no algorithms for...

  15. Binary dicots, a core of dicot games

    OpenAIRE

    Renault, Gabriel

    2014-01-01

    We study combinatorial games under mis\\`ere convention. Several sets of games have been considered earlier to better understand the behaviour of mis\\`ere games. We here connect several of these sets. In particular, we prove that comparison modulo binary dicot games is often the same as comparison modulo dicot games, and that equivalence modulo dicot games and modulo impartial games are the same when they are restricted to impartial games.

  16. Modified Sonine approximation for granular binary mixtures

    OpenAIRE

    Garzó, Vicente; Reyes, Francisco Vega; Montanero, José María

    2008-01-01

    We evaluate in this work the hydrodynamic transport coefficients of a granular binary mixture in $d$ dimensions. In order to eliminate the observed disagreement (for strong dissipation) between computer simulations and previously calculated theoretical transport coefficients for a monocomponent gas, we obtain explicit expressions of the seven Navier-Stokes transport coefficients with the use of a new Sonine approach in the Chapman-Enskog theory. Our new approach consists in replacing, where a...

  17. Mergers of Binary Neutron Star Systems

    Science.gov (United States)

    Motl, Patrick M.; Anderson, Matthew; Lehner, Luis; Liebling, Steven; Neilsen, David; Palenzuela, Carlos

    2016-04-01

    We present results from fully relativistic simulations of binary neutron star mergers varying the tabular equation of state used to approximate the degenerate material and the mass ratio. The simulations incorporate both magnetic fields and the effects of neutrino cooling. In particular, we examine the amount and properties of material ejected from the merger. We gratefully acknowledge the support of NASA through the Astrophysics Theory Program grant NNX13AH01G.

  18. Dynamics of phase separation of binary fluids

    Science.gov (United States)

    Ma, Wen-Jong; Maritan, Amos; Banavar, Jayanth R.; Koplik, Joel

    1992-01-01

    The results of molecular-dynamics studies of surface-tension-dominated spinodal decomposition of initially well-mixed binary fluids in the absence and presence of gravity are presented. The growth exponent for the domain size and the decay exponent of the potential energy of interaction between the two species with time are found to be 0.6 +/- 0.1, inconsistent with scaling arguments based on dimensional analysis.

  19. The Binary Choice Approach of Laffer Curve

    OpenAIRE

    Mihai Mutascu

    2012-01-01

    The paper analyzes empirically, based on “Laffer effects”, in Romania’s case, the relationship between tax revenues (dependent variable) and tax rates (independent variables). The analysis is based on the construction of a binary choice model (Linear Probit Model) and the data set is covering the period 1999 - 2009 (first trimester), with quarterly frequency. The main results show that the two “Laffer effects” have a different probability of existence. If the government knows which the maximu...

  20. Pressure effects in multiphase binary diffusion couples

    Science.gov (United States)

    Subramanyam, Dilip; Notis, Michael R.; Goldstein, Joseph I.

    1985-04-01

    A systematic study has been carried out of the effect of pressure upon growth kinetics of intermediate phases formed in diffusion couples in the binary systems Ni-Al, U-A1, and U-Cu. Even though applied pressures greater than 100 MPa and long times were investigated little or no pressure effect was observed, in disagreement with previous literature reports. The magnitude of observed pressure effects falls within that expected by closure of Kirkendall porosity.

  1. Relativistic apsidal motion in eccentric eclipsing binaries

    Czech Academy of Sciences Publication Activity Database

    Wolf, M.; Claret, L.; Kotková, Lenka; Kučáková, H.; Kocián, R.; Brát, L.; Svoboda, P.; Šmelcer, L.

    2010-01-01

    Roč. 509, January (2010), A18/1-A18/14. ISSN 0004-6361 Grant ostatní: GA ČR(CZ) GA205/04/2063; GA ČR(CZ) GA205/06/0217 Institutional research plan: CEZ:AV0Z10030501 Keywords : binaries eclipsing Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 4.410, year: 2010

  2. Binary magnetic structures in HoEr

    DEFF Research Database (Denmark)

    Howard, B.K.; Bohr, J.

    observed between 104 K and 47.5 K is a binary magnetic structure where the holmium and erbium moments belong to different modulated c-axis spirals. The intermediate-temperature phase between 47.5 K and 35 K is a simple basal plane spiral. Below 35 K, the measurements suggest a ferrimagnetic structure in...... which the holmium and erbium moments are indistinguishable and form a conical sprial with a half-cone angle of 20-degrees....

  3. Outbursts in ultracompact X-ray binaries

    CERN Document Server

    Hameury, J -M

    2016-01-01

    Very faint X-ray binaries appear to be transient in many cases with peak luminosities much fainter than that of usual soft X-ray transients, but their nature still remains elusive. We investigate the possibility that this transient behaviour is due to the same thermal/viscous instability which is responsible for outbursts of bright soft X-ray transients, occurring in ultracompact binaries for adequately low mass-transfer rates. More generally, we investigate the observational consequences of this instability when it occurs in ultracompact binaries. We use our code for modelling the thermal-viscous instability of the accretion disc, assumed here to be hydrogen poor. We also take into account the effects of disc X-ray irradiation, and consider the impact of the mass-transfer rate on the outburst brightness. We find that one can reproduce the observed properties of both the very faint and the brighter short transients (peak luminosity, duration, recurrence times), provided that the viscosity parameter in quiesce...

  4. A radio pulsing white dwarf binary star

    CERN Document Server

    Marsh, T R; Hümmerich, S; Hambsch, F -J; Bernhard, K; Lloyd, C; Breedt, E; Stanway, E R; Steeghs, D T; Parsons, S G; Toloza, O; Schreiber, M R; Jonker, P G; van Roestel, J; Kupfer, T; Pala, A F; Dhillon, V S; Hardy, L K; Littlefair, S P; Aungwerojwit, A; Arjyotha, S; Koester, D; Bochinski, J J; Haswell, C A; Frank, P; Wheatley, P J

    2016-01-01

    White dwarfs are compact stars, similar in size to Earth but ~200,000 times more massive. Isolated white dwarfs emit most of their power from ultraviolet to near-infrared wavelengths, but when in close orbits with less dense stars, white dwarfs can strip material from their companions, and the resulting mass transfer can generate atomic line and X-ray emission, as well as near- and mid-infrared radiation if the white dwarf is magnetic. However, even in binaries, white dwarfs are rarely detected at far-infrared or radio frequencies. Here we report the discovery of a white dwarf / cool star binary that emits from X-ray to radio wavelengths. The star, AR Scorpii (henceforth AR Sco), was classified in the early 1970s as a delta-Scuti star, a common variety of periodic variable star. Our observations reveal instead a 3.56 hr period close binary, pulsing in brightness on a period of 1.97 min. The pulses are so intense that AR Sco's optical flux can increase by a factor of four within 30 s, and they are detectable a...

  5. Spinodal decomposition of chemically reactive binary mixtures

    Science.gov (United States)

    Lamorgese, A.; Mauri, R.

    2016-08-01

    We simulate the influence of a reversible isomerization reaction on the phase segregation process occurring after spinodal decomposition of a deeply quenched regular binary mixture, restricting attention to systems wherein material transport occurs solely by diffusion. Our theoretical approach follows a diffuse-interface model of partially miscible binary mixtures wherein the coupling between reaction and diffusion is addressed within the frame of nonequilibrium thermodynamics, leading to a linear dependence of the reaction rate on the chemical affinity. Ultimately, the rate for an elementary reaction depends on the local part of the chemical potential difference since reaction is an inherently local phenomenon. Based on two-dimensional simulation results, we express the competition between segregation and reaction as a function of the Damköhler number. For a phase-separating mixture with components having different physical properties, a skewed phase diagram leads, at large times, to a system converging to a single-phase equilibrium state, corresponding to the absolute minimum of the Gibbs free energy. This conclusion continues to hold for the critical phase separation of an ideally perfectly symmetric binary mixture, where the choice of final equilibrium state at large times depends on the initial mean concentration being slightly larger or less than the critical concentration.

  6. A field guide to the binary stars

    Science.gov (United States)

    Trimble, V.

    1983-05-01

    Details and examples of the six phases of existence for a binary star system are described. The birth and pre-main-sequence contraction is generally obscured from observation by the presence of gas and dust clouds; it comprises 1/1000th of a system's lifetime. The main sequence, i.e., hydrogen burning, takes up to 90 pct of a star's lifetime, and has been detected in stars with masses ranging from 0.07-32 solar masses. In binary systems, the main sequence stars may or may not interact, or one companion may burn out before the other leaves the main sequence. The primary in a binary system expands to fill its Roche lobe before mass transfer begins, then continues on a Kelvin-Helmholtz time scale until the primary is smaller than the secondary, when transfer proceeds on a nuclear time scale. The depletion of hydrogen fuel or He ignition stops the mass transfer, leading to formation of a white dwarf, neutron star, or supernova that sends both the neutron star and the OB secondary off at high speeds. Back transfer can be initiated in a fifth phase and can produce black holes or dwarf novae, or supernovae. Finally, the system terminates when both stars are extinguished and fall into one another, which can also yield supernovae or black holes.

  7. On the binary expansions of algebraic numbers

    Energy Technology Data Exchange (ETDEWEB)

    Bailey, David H.; Borwein, Jonathan M.; Crandall, Richard E.; Pomerance, Carl

    2003-07-01

    Employing concepts from additive number theory, together with results on binary evaluations and partial series, we establish bounds on the density of 1's in the binary expansions of real algebraic numbers. A central result is that if a real y has algebraic degree D > 1, then the number {number_sign}(|y|, N) of 1-bits in the expansion of |y| through bit position N satisfies {number_sign}(|y|, N) > CN{sup 1/D} for a positive number C (depending on y) and sufficiently large N. This in itself establishes the transcendency of a class of reals {summation}{sub n{ge}0} 1/2{sup f(n)} where the integer-valued function f grows sufficiently fast; say, faster than any fixed power of n. By these methods we re-establish the transcendency of the Kempner--Mahler number {summation}{sub n{ge}0}1/2{sup 2{sup n}}, yet we can also handle numbers with a substantially denser occurrence of 1's. Though the number z = {summation}{sub n{ge}0}1/2{sup n{sup 2}} has too high a 1's density for application of our central result, we are able to invoke some rather intricate number-theoretical analysis and extended computations to reveal aspects of the binary structure of z{sup 2}.

  8. A Gray path on binary partitions

    CERN Document Server

    Colthurst, Thomas

    2009-01-01

    A binary partition of a positive integer $n$ is a partition of $n$ in which each part has size a power of two. In this note we first construct a Gray sequence on the set of binary partitions of $n$. This is an ordering of the set of binary partitions of each $n$ (or of all $n$) such that adjacent partitions differ by one of a small set of elementary transformations; here the allowed transformatios are replacing $2^k+2^k$ by $2^{k+1}$ or vice versa (or addition of a new +1). Next we give a purely local condition for finding the successor of any partition in this sequence; the rule is so simple that successive transitions can be performed in constant time. Finally we show how to compute directly the bijection between $k$ and the $k$th term in the sequence. This answers a question posed by Donald Knuth in section 7.2.1 of The Art of Computer Programming.

  9. Circumstellar disks around binary stars in Taurus

    International Nuclear Information System (INIS)

    We have conducted a survey of 17 wide (>100 AU) young binary systems in Taurus with the Atacama Large Millimeter Array (ALMA) at two wavelengths. The observations were designed to measure the masses of circumstellar disks in these systems as an aid to understanding the role of multiplicity in star and planet formation. The ALMA observations had sufficient resolution to localize emission within the binary system. Disk emission was detected around all primaries and 10 secondaries, with disk masses as low as 10–4 M ☉. We compare the properties of our sample to the population of known disks in Taurus and find that the disks from this binary sample match the scaling between stellar mass and millimeter flux of Fmm∝M∗1.5--2.0 to within the scatter found in previous studies. We also compare the properties of the primaries to those of the secondaries and find that the secondary/primary stellar and disk mass ratios are not correlated; in three systems, the circumsecondary disk is more massive than the circumprimary disk, counter to some theoretical predictions.

  10. Circumstellar disks around binary stars in Taurus

    Energy Technology Data Exchange (ETDEWEB)

    Akeson, R. L. [NASA Exoplanet Science Institute, IPAC/Caltech, Pasadena, CA 91125 (United States); Jensen, E. L. N. [Swarthmore College, Department of Physics and Astronomy, Swarthmore, PA 19081 (United States)

    2014-03-20

    We have conducted a survey of 17 wide (>100 AU) young binary systems in Taurus with the Atacama Large Millimeter Array (ALMA) at two wavelengths. The observations were designed to measure the masses of circumstellar disks in these systems as an aid to understanding the role of multiplicity in star and planet formation. The ALMA observations had sufficient resolution to localize emission within the binary system. Disk emission was detected around all primaries and 10 secondaries, with disk masses as low as 10{sup –4} M {sub ☉}. We compare the properties of our sample to the population of known disks in Taurus and find that the disks from this binary sample match the scaling between stellar mass and millimeter flux of F{sub mm}∝M{sub ∗}{sup 1.5--2.0} to within the scatter found in previous studies. We also compare the properties of the primaries to those of the secondaries and find that the secondary/primary stellar and disk mass ratios are not correlated; in three systems, the circumsecondary disk is more massive than the circumprimary disk, counter to some theoretical predictions.

  11. Binary progenitor models of type IIb supernovae

    CERN Document Server

    Claeys, J S W; Pols, O R; Eldridge, J J; Baes, M

    2011-01-01

    Massive stars that lose their hydrogen-rich envelope down to a few tenths of a solar mass explode as extended type IIb supernovae, an intriguing subtype that links the hydrogen-rich type II supernovae with the hydrogen-poor type Ib and Ic. The progenitors may be very massive single stars that lose their envelope due to their stellar wind, but mass stripping due to interaction with a companion star in a binary system is currently considered to be the dominant formation channel. We computed an extensive grid of binary models with the Eggleton binary evolution code. The predicted rate from our standard models, which assume conservative mass transfer, is about 6 times smaller than the current rate indicated by observations. It is larger but still comparable to the rate expected from single stars. To recover the observed rate we must generously allow for uncertainties and low accretion efficiencies in combination with limited angular momentum loss from the system. Motivated by the claims of detection and non-detec...

  12. Observational signatures of binary supermassive black holes

    International Nuclear Information System (INIS)

    Observations indicate that most massive galaxies contain a supermassive black hole, and theoretical studies suggest that when such galaxies have a major merger, the central black holes will form a binary and eventually coalesce. Here we discuss two spectral signatures of such binaries that may help distinguish them from ordinary active galactic nuclei. These signatures are expected when the mass ratio between the holes is not extreme and the system is fed by a circumbinary disk. One such signature is a notch in the thermal continuum that has been predicted by other authors; we point out that it should be accompanied by a spectral revival at shorter wavelengths and also discuss its dependence on binary properties such as mass, mass ratio, and separation. In particular, we note that the wavelength λ n at which the notch occurs depends on these three parameters in such a way as to make the number of systems displaying these notches ∝λn16/3; longer wavelength searches are therefore strongly favored. A second signature, first discussed here, is hard X-ray emission with a Wien-like spectrum at a characteristic temperature ∼100 keV produced by Compton cooling of the shock generated when streams from the circumbinary disk hit the accretion disks around the individual black holes. We investigate the observability of both signatures. The hard X-ray signal may be particularly valuable as it can provide an indicator of black hole merger a few decades in advance of the event.

  13. On the Neutron Star-Black Hole Binaries Produced by Binary-driven Hypernovae

    CERN Document Server

    Fryer, C L; Rueda, J A; Ruffini, R

    2015-01-01

    Binary-driven hypernovae (BdHNe) following the induced gravitational collapse (IGC) paradigm have been introduced to explain the concomitance of energetic long gamma-ray bursts (GRBs) with type Ic supernovae. The progenitor system is a tight binary system composed of a carbon-oxygen (CO) core and a neutron star (NS) companion. The supernova ejecta of the exploding CO core triggers a hypercritical accretion process onto the NS, which in a few seconds reach the NS critical mass, and gravitationally collapses to a black hole (BH) emitting a GRB. These tight binary systems evolve through the supernova explosion very differently than compact binary progenitors studied in population synthesis calculations. First, the hypercritical accretion onto the NS companion alters both the mass and momentum of the binary. Second, because the explosion timescale is on par with the orbital period, the mass ejection can not be assumed to be instantaneous. Finally, the bow shock created as the accreting NS plows through the supern...

  14. A Survey Design for a Sensitive Binary Variable Correlated with Another Nonsensitive Binary Variable

    Directory of Open Access Journals (Sweden)

    Jun-Wu Yu

    2013-01-01

    Full Text Available Tian et al. (2007 introduced a so-called hidden sensitivity model for evaluating the association of two sensitive questions with binary outcomes. However, in practice, we sometimes need to assess the association between one sensitive binary variable (e.g., whether or not a drug user, the number of sex partner being ⩽1 or >1, and so on and one nonsensitive binary variable (e.g., good or poor health status, with or without cervical cancer, and so on. To address this issue, by sufficiently utilizing the information contained in the non-sensitive binary variable, in this paper, we propose a new survey scheme, called combination questionnaire design/model, which consists of a main questionnaire and a supplemental questionnaire. The introduction of the supplemental questionnaire which is indeed a design of direct questioning can effectively reduce the noncompliance behavior since more respondents will not be faced with the sensitive question. Likelihood-based inferences including maximum likelihood estimates via the expectation-maximization algorithm, asymptotic confidence intervals, and bootstrap confidence intervals of parameters of interest are derived. A likelihood ratio test is provided to test the association between the two binary random variables. Bayesian inferences are also discussed. Simulation studies are performed, and a cervical cancer data set in Atlanta is used to illustrate the proposed methods.

  15. The formation of eccentric compact binary inspirals and the role of gravitational wave emission in binary-single stellar encounters

    Energy Technology Data Exchange (ETDEWEB)

    Samsing, Johan [Dark Cosmology Centre, Niels Bohr Institute, University of Copenhagen, Juliane Maries Vej 30, DK-2100 Copenhagen (Denmark); MacLeod, Morgan; Ramirez-Ruiz, Enrico [Department of Astronomy and Astrophysics, University of California, Santa Cruz, CA 95064 (United States)

    2014-03-20

    The inspiral and merger of eccentric binaries leads to gravitational waveforms distinct from those generated by circularly merging binaries. Dynamical environments can assemble binaries with high eccentricity and peak frequencies within the LIGO band. In this paper, we study binary-single stellar scatterings occurring in dense stellar systems as a source of eccentrically inspiraling binaries. Many interactions between compact binaries and single objects are characterized by chaotic resonances in which the binary-single system undergoes many exchanges before reaching a final state. During these chaotic resonances, a pair of objects has a non-negligible probability of experiencing a very close passage. Significant orbital energy and angular momentum are carried away from the system by gravitational wave (GW) radiation in these close passages, and in some cases this implies an inspiral time shorter than the orbital period of the bound third body. We derive the cross section for such dynamical inspiral outcomes through analytical arguments and through numerical scattering experiments including GW losses. We show that the cross section for dynamical inspirals grows with increasing target binary semi-major axis a and that for equal-mass binaries it scales as a {sup 2/7}. Thus, we expect wide target binaries to predominantly contribute to the production of these relativistic outcomes. We estimate that eccentric inspirals account for approximately 1% of dynamically assembled non-eccentric merging binaries. While these events are rare, we show that binary-single scatterings are a more effective formation channel than single-single captures for the production of eccentrically inspiraling binaries, even given modest binary fractions.

  16. Learning Compact Binary Face Descriptor for Face Recognition.

    Science.gov (United States)

    Lu, Jiwen; Liong, Venice Erin; Zhou, Xiuzhuang; Zhou, Jie

    2015-10-01

    Binary feature descriptors such as local binary patterns (LBP) and its variations have been widely used in many face recognition systems due to their excellent robustness and strong discriminative power. However, most existing binary face descriptors are hand-crafted, which require strong prior knowledge to engineer them by hand. In this paper, we propose a compact binary face descriptor (CBFD) feature learning method for face representation and recognition. Given each face image, we first extract pixel difference vectors (PDVs) in local patches by computing the difference between each pixel and its neighboring pixels. Then, we learn a feature mapping to project these pixel difference vectors into low-dimensional binary vectors in an unsupervised manner, where 1) the variance of all binary codes in the training set is maximized, 2) the loss between the original real-valued codes and the learned binary codes is minimized, and 3) binary codes evenly distribute at each learned bin, so that the redundancy information in PDVs is removed and compact binary codes are obtained. Lastly, we cluster and pool these binary codes into a histogram feature as the final representation for each face image. Moreover, we propose a coupled CBFD (C-CBFD) method by reducing the modality gap of heterogeneous faces at the feature level to make our method applicable to heterogeneous face recognition. Extensive experimental results on five widely used face datasets show that our methods outperform state-of-the-art face descriptors. PMID:26340256

  17. Merging binary black holes formed through chemically homogeneous evolution in short-period stellar binaries

    CERN Document Server

    Mandel, Ilya

    2016-01-01

    We explore a newly proposed channel to create binary black holes of stellar origin. This scenario applies to massive, tight binaries where mixing induced by rotation and tides transports the products of hydrogen burning throughout the stellar envelopes. This slowly enriches the entire star with helium, preventing the build-up of an internal chemical gradient. The stars remain compact as they evolve nearly chemically homogeneously, eventually forming two black holes, which, we estimate, typically merge 4 to 11 Gyr after formation. Like other proposed channels, this evolutionary pathway suffers from significant theoretical uncertainties, but could be constrained in the near future by data from advanced ground-based gravitational-wave detectors. We perform Monte Carlo simulations of the expected merger rate over cosmic time to explore the implications and uncertainties. Our default model for this channel yields a local binary black hole merger rate of about $10$ Gpc$^{-3}$ yr$^{-1}$ at redshift $z=0$, peaking at...

  18. A precontact binary and a shallow contact binary are in the same field

    Science.gov (United States)

    Liu, Liang; Qian, Shengbang; He, Jiajia; Liao, Wenping; Liu, Nianping

    2016-06-01

    The period changes of two close binaries, V1107 Cas and AX Cas, which are in the same field, were investigated. Their periods both show a long-term decrease. After further analysis, we found that the periods have their respective cyclic oscillations (T3 = 6.74 ± 0.24 yr for V1107 Cas and T3 = 13.8 ± 0.3 yr for AX Cas), which are possibly caused by a third body due to the light-time effect. We also obtained the complete VRcIc light curves for V1107 Cas and analyzed them with the 2010 version of the Wilson-Devinney code. The photometric results reveal that V1107 Cas is a W-type shallow contact (15.2%±1.8%) binary, with a mass-ratio of 1.797 ± 0.006. The period variation and photometric solution suggest that V1107 Cas is a newly formed contact binary system. Moreover, we estimated the fundamental parameters for V1107 Cas. They are: M1 = 0.39 ± 0.01 M⊙, M2 = 0.70 ± 0.03 M⊙, R1 = 0.52 ± 0.10 R⊙, R2 = 0.68 ± 0.12 R⊙, L1 = 0.178 ± 0.108 L⊙, and L2 = 0.196 ± 0.116 L⊙. Then, based on the coplane assumption, we deduced the masses of possible third bodies to be M3 = 0.091 ± 0.019 M⊙ for V1107 Cas and M3 = 0.325 ± 0.029 M⊙ for AX Cas. Finally, we inferred the evolutional stage of AX Cas, and believe that it is a precontact binary. Thus, the precontact binary AX Cas and the shallow contact binary V1107 Cas have adjoining evolutional stages.

  19. Properties of eclipsing binaries from all-sky surveys - I. Detached eclipsing binaries in ASAS, NSVS, and LINEAR

    Science.gov (United States)

    Lee ( ), Chien-Hsiu

    2015-11-01

    Eclipsing binaries provide a unique opportunity to measure fundamental properties of stars. With the advent of all-sky surveys, thousands of eclipsing binaries have been reported, yet their light curves are not fully exploited. The goal of this work is to make use of the eclipsing binary light curves delivered by all-sky surveys. We attempt to extract physical parameters of the binary systems from their light curves and colour. Inspired by the work of Devor et al., we use the Detached Eclipsing Binary Light curve fitter (DEBIL) and the Method for Eclipsing Component Identification (MECI) to derive basic properties of the binary systems reported by the All Sky Automated Survey, the Northern Sky Variability Survey, and the Lincoln Near Earth Asteroids Research. We derive the mass, fractional radius, and age for 783 binary systems. We report a subsample of eccentric systems and compare their properties to the tidal circularization theory. With MECI, we are able to estimate the distance of the eclipsing binary systems and use them to probe the structure of the Milky Way. Following the approach of Devor et al., we demonstrate that DEBIL and MECI are instrumental to investigate eclipsing binary light curves in the era of all-sky surveys, and provide estimates of stellar parameters of both binary components without spectroscopic information.

  20. Binary pairs of supermassive black holes - Formation in merging galaxies

    International Nuclear Information System (INIS)

    A process in which supermassive binary blackholes are formed in nuclei of supergiant galaxies due to galaxy mergers is examined. There is growing evidence that mergers of galaxies are common and that supermassive black holes in center of galaxies are also common. Consequently, it is expected that binary black holes should arise in connection with galaxy mergers. The merger process in a galaxy modeled after M87 is considered. The capture probability of a companion is derived as a function of its mass. Assuming a correlation between the galaxy mass and the blackholes mass, the expected mass ratio in binary black holes is calculated. The binary black holes formed in this process are long lived, surviving longer than the Hubble time unless they are perturbed by black holes from successive mergers. The properties of these binaries agree with Gaskell's (1988) observational work on quasars and its interpretation in terms of binary black holes. 39 refs

  1. Binary pulsar evolution: unveiled links and new species

    Science.gov (United States)

    Possenti, Andrea

    2013-03-01

    In the last years a series of blind and/or targeted pulsar searches led to almost triple the number of known binary pulsars in the galactic field with respect to a decade ago. The focus will be on few outliers, which are emerging from the average properties of the enlarged binary pulsar population. Some of them may represent the long sought missing links between two kinds of neutron star binaries, while others could represent the stereotype of new groups of binaries, resulting from an evolutionary path which is more exotic than those considered until recently. In particular, a new class of binaries, which can be dubbed Ultra Low Mass Binary Pulsars (ULMBPs), is emerging from recent data.

  2. Microlensing Binaries Discovered through High-magnification Channel

    DEFF Research Database (Denmark)

    Shin, I.-G.; Choi, J.-Y.; Park, S.-Y.;

    2012-01-01

    Microlensing can provide a useful tool to probe binary distributions down to low-mass limits of binary companions. In this paper, we analyze the light curves of eight binary-lensing events detected through the channel of high-magnification events during the seasons from 2007 to 2010. The perturba......Microlensing can provide a useful tool to probe binary distributions down to low-mass limits of binary companions. In this paper, we analyze the light curves of eight binary-lensing events detected through the channel of high-magnification events during the seasons from 2007 to 2010...... of the event OGLE-2008-BLG-510/MOA-2008-BLG-369 is q ~ 0.1, making the companion of the lens a strong brown dwarf candidate....

  3. Milankovitch Cycles of Terrestrial Planets in Binary Star Systems

    CERN Document Server

    Forgan, Duncan H

    2016-01-01

    The habitability of planets in binary star systems depends not only on the radiation environment created by the two stars, but also on the perturbations to planetary orbits and rotation produced by the gravitational field of the binary and neighbouring planets. Habitable planets in binaries may therefore experience significant perturbations in orbit and spin. The direct effects of orbital resonances and secular evolution on the climate of binary planets remain largely unconsidered. We present latitudinal energy balance modelling of exoplanet climates with direct coupling to an N Body integrator and an obliquity evolution model. This allows us to simultaneously investigate the thermal and dynamical evolution of planets orbiting binary stars, and discover gravito-climatic oscillations on dynamical and secular timescales. We investigate the Kepler-47 and Alpha Centauri systems as archetypes of P and S type binary systems respectively. In the first case, Earthlike planets would experience rapid Milankovitch cycle...

  4. Distinguishing Between Formation Channels for Binary Black Holes with LISA

    CERN Document Server

    Breivik, Katelyn; Larson, Shane L; Kalogera, Vassiliki; Rasio, Frederic A

    2016-01-01

    The recent detections of GW150914 and GW151226 imply an abundance of stellar-mass binary-black-hole mergers in the local universe. While ground-based gravitational-wave detectors are limited to observing the final moments before a binary merges, space-based detectors, such as the Laser Interferometer Space Antenna (LISA), can observe binaries at lower orbital frequencies where such systems may still encode information about their formation histories. In particular, the orbital eccentricity of binary black holes in the LISA frequency band can be used discriminate between binaries formed in isolation in galactic fields, and those formed in dense stellar environments such as globular clusters. In this letter, we explore the differences in orbital eccentricities of binary black hole populations as they evolve through the LISA frequency band. Overall we find that there are three distinct populations of orbital eccentricities discernible by LISA. We show that, depending on gravitational-wave frequency, anywhere fro...

  5. Nonlinear Dynamics, Lorenz Model and Formation of Binary Stars

    OpenAIRE

    Chang, Yi-Fang

    2008-01-01

    Based on the Lorenz model derived from the equations of hydrodynamics of nebula, we discuss the formation of binary stars by the qualitative analysis theory of nonlinear equation. Here the two wings in the Lorenz model form just the binary stars, whose Roche surface is result of evolution under certain condition. The nonlinear interaction plays a crucial role, and is necessary condition of the formation of binary stars and of multiple stars. While the linear equations form only a single star....

  6. On the formation of Be stars through binary interaction

    OpenAIRE

    Shao, Yong; Li, Xiang-Dong

    2014-01-01

    Be stars are rapidly rotating B type stars. The origin of their rapid rotation is not certain, but binary interaction remains to be a possibility. In this work we investigate the formation of Be stars resulting from mass transfer in binaries in the Galaxy. We calculate the binary evolution with both stars evolving simultaneously and consider different possible mass accretion histories for the accretor. From the calculated results we obtain the critical mass ratios $q_{\\rm cr}$ that determine ...

  7. Magnetic Interaction in Ultra-compact Binary Systems

    CERN Document Server

    Wu, Kinwah

    2009-01-01

    This article reviews the current works on ultra-compact double-degenerate binaries in the presence of magnetic interaction, in particular, unipolar induction. The orbital dynamics and evolution of compact white-dwarf pairs are discussed in detail. Models and predictions of electron cyclotron masers from unipolar-inductor compact binaries and unipolar-inductor white-dwarf planetary systems are presented. Einstein-Laub effects in compact binaries are briefly discussed.

  8. Magnetic interaction in ultra-compact binary systems

    Institute of Scientific and Technical Information of China (English)

    Kinwah WU

    2009-01-01

    This article reviews the current works on ultra-compact double-degenerate binaries in the presence of magnetic interaction, in particular, unipolar induction. The orbital dynamics and evolution of compact white-dwarf pairs are discussed in detail. Models and predictions of electron cyclotron masers from unipolar-inductor compact binaries and unipolar-inductor white-dwarf planetary systems are presented. Einstein-Laub effects in compact binaries are briefly discussed.

  9. Automatic classification of eclipsing binaries light curves using neural networks

    CERN Document Server

    Sarro, L M; Giménez, A

    2005-01-01

    In this work we present a system for the automatic classification of the light curves of eclipsing binaries. This system is based on a classification scheme that aims to separate eclipsing binary sistems according to their geometrical configuration in a modified version of the traditional classification scheme. The classification is performed by a Bayesian ensemble of neural networks trained with {\\em Hipparcos} data of seven different categories including eccentric binary systems and two types of pulsating light curve morphologies.

  10. How I Learned to Stop Worrying and Love Eclipsing Binaries

    OpenAIRE

    Moe, Maxwell

    2015-01-01

    Relatively massive B-type stars with closely orbiting stellar companions can evolve to produce Type Ia supernovae, X-ray binaries, millisecond pulsars, mergers of neutron stars, gamma ray bursts, and sources of gravitational waves. However, the formation mechanism, intrinsic frequency, and evolutionary processes of B-type binaries are poorly understood. As of 2012, the binary statistics of massive stars had not been measured at low metallicities, extreme mass ratios, or intermediate orbital ...

  11. NEW BINARY PARTICLE SWARM OPTIMIZATION WITH IMMUNITY-CLONAL ALGORITHM

    OpenAIRE

    Dina EL-Gammal; Amr Badr; Mostafa Abd El Azeim

    2013-01-01

    Particle Swarm Optimization used to solve a continuous problem and has been shown to perform well however, binary version still has some problems. In order to solve these problems a new technique called New Binary Particle Swarm Optimization using Immunity-Clonal Algorithm (NPSOCLA) is proposed This Algorithm proposes a new updating strategy to update the position vector in Binary Particle Swarm Optimization (BPSO), which further combined with Immunity-Clonal Algorithm to improve the optimiza...

  12. Information Measures: the Curious Case of the Binary Alphabet

    OpenAIRE

    Jiao, Jiantao; Courtade, Thomas; No, Albert; Venkat, Kartik; Weissman, Tsachy

    2014-01-01

    Four problems related to information divergence measures defined on finite alphabets are considered. In three of the cases we consider, we illustrate a contrast which arises between the binary-alphabet and larger-alphabet settings. This is surprising in some instances, since characterizations for the larger-alphabet settings do not generalize their binary-alphabet counterparts. Specifically, we show that $f$-divergences are not the unique decomposable divergences on binary alphabets that sati...

  13. Simple methods for evaluating and comparing binary experiments

    OpenAIRE

    Thomas A. Weber

    2010-01-01

    We consider a confidence parametrization of binary information sources in terms of appropriate likelihood ratios. This parametrization is used for Bayesian belief updates and for the equivalent comparison of binary experiments. In contrast to the standard parametrization of a binary information source in terms of its specificity and its sensitivity, one of the two confidence parameters is sufficient for a Bayesian belief update conditional on a signal realization. We introduce a confidence-au...

  14. Segregation phases in a vibrated binary granular layer

    OpenAIRE

    Reis, P. M.; Ehrhardt, G.; Mullin, T.

    2003-01-01

    We present the results of an experimental study of patterned segregation in a horizontally shaken shallow layer of a binary mixture of dry particles. As the compacity, $C$, of the mixture was increased, the evolution of three distinct phases was observed. We classify them as binary gas, segregation liquid and segregation crystal phases using macroscopic and microscopic measures. The binary gas to segregation liquid transition is consistent with a continuous phase transition and includes the c...

  15. Geographically Weighted Local Statistics Applied to Binary Data

    OpenAIRE

    Brunsdon, Chris; Fotheringham, Stewart; Charlton, Martin

    2002-01-01

    This paper considers the application of geographically weighting to summary statistics for binary data. We argue that geographical smoothing techniques that are applied to descriptive statistics for ratio and interval scale data may also be applied to descriptive statistics for binary categorical data. Here we outline how this may be done, focussing attention on the odds ratio statistic used for summarising the linkage between a pair of binary variables. An example of this is applied to data ...

  16. Asymptotic bound on binary self-orthogonal codes

    Institute of Scientific and Technical Information of China (English)

    DING Yang

    2009-01-01

    We present two constructions for binary self-orthogonal codes. It turns out that our constructions yield a constructive bound on binary self-orthogonal codes. In particular, when the in-formation rate R = 1/2, by our constructive lower bound, the relative minimum distance δ≈ 0.0595 (for GV bound, δ≈0.110). Moreover, we have proved that the binary self-orthogonal codes asymptotically achieve the Gilbert-Varshamov bound.

  17. Asymptotic bound on binary self-orthogonal codes

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    We present two constructions for binary self-orthogonal codes. It turns out that our constructions yield a constructive bound on binary self-orthogonal codes. In particular, when the in-formation rate R = 1/2, by our constructive lower bound, the relative minimum distance δ≈ 0.0595 (for GV bound, δ≈ 0.110). Moreover, we have proved that the binary self-orthogonal codes asymptotically achieve the Gilbert-Varshamov bound.

  18. Evolution of a ring around the Pluto-Charon binary

    OpenAIRE

    Bromley, B. C.; Kenyon, S J

    2015-01-01

    We consider the formation of satellites around the Pluto-Charon binary. An early collision between the two partners likely produced the binary and a narrow ring of debris, out of which arose the moons Styx, Nix, Kerberos and Hydra. How the satellites emerged from the compact ring is uncertain. Here we show that a particle ring spreads from physical collisions and collective gravitational scattering, similar to migration. Around a binary, these processes take place in the reference frames of "...

  19. Improving geothermal power plants with a binary cycle

    Science.gov (United States)

    Tomarov, G. V.; Shipkov, A. A.; Sorokina, E. V.

    2015-12-01

    The recent development of binary geothermal technology is analyzed. General trends in the introduction of low-temperature geothermal sources are summarized. The use of single-phase low-temperature geothermal fluids in binary power plants proves possible and expedient. The benefits of power plants with a binary cycle in comparison with traditional systems are shown. The selection of the working fluid is considered, and the influence of the fluid's physicochemical properties on the design of the binary power plant is discussed. The design of binary power plants is based on the chemical composition and energy potential of the geothermal fluids and on the landscape and climatic conditions at the intended location. Experience in developing a prototype 2.5 MW Russian binary power unit at Pauzhetka geothermal power plant (Kamchatka) is outlined. Most binary systems are designed individually for a specific location. Means of improving the technology and equipment at binary geothermal power plants are identified. One option is the development of modular systems based on several binary systems that employ the heat from the working fluid at different temperatures.

  20. DESTRUCTION OF BINARY MINOR PLANETS DURING NEPTUNE SCATTERING

    International Nuclear Information System (INIS)

    The existence of extremely wide binaries in the low-inclination component of the Kuiper Belt provides a unique handle on the dynamical history of this population. Some popular frameworks of the formation of the Kuiper Belt suggest that planetesimals were moved there from lower semimajor axis orbits by scattering encounters with Neptune. We test the effects such events would have on binary systems and find that wide binaries are efficiently destroyed by the kinds of scattering events required to create the Kuiper Belt with this mechanism. This indicates that a binary-bearing component of the cold Kuiper Belt was emplaced through a gentler mechanism or was formed in situ.

  1. ANNEXATION OF TWO KINDS OF SOLUTION IN BINARY METALLIC MELTS

    Institute of Scientific and Technical Information of China (English)

    J.Zhang

    2004-01-01

    After investigation on the thervnodynamic properties of a small number of binary metallic melts,the structural units of which cannot be wholly determined by the corresponding phase diagrams,it was found that they can be determined by the principle of annexation of two kinds of solutions in binary metallic melts.According to the principle of annexation,calculating models of mass action concentrations for several binary metallic melts have been formulated.The calculated results agree well with practice,showing that this principle is a reliable basis for determination of the structural units for some binary metallic melts.

  2. Binary GCD like Algorithms for Some Complex Quadratic Rings

    DEFF Research Database (Denmark)

    Agarwal, Saurabh; Frandsen, Gudmund Skovbjerg

    2004-01-01

    binary gcd like algorithms for the ring of integers in and , one now has binary gcd like algorithms for all complex quadratic Euclidean domains. The running time of our algorithms is O(n 2) in each ring. While there exists an O(n 2) algorithm for computing the gcd in quadratic number rings by Erich......On the lines of the binary gcd algorithm for rational integers, algorithms for computing the gcd are presented for the ring of integers in where . Thus a binary gcd like algorithm is presented for a unique factorization domain which is not Euclidean (case d=-19). Together with the earlier known...

  3. White-Light Flares on Close Binaries Observed with Kepler

    CERN Document Server

    Gao, Qing; Liu, Ji-Feng; Zhang, Xiao-Bin; Gao, Shuang

    2016-01-01

    Based on Kepler data, we present the results of a search for white-light flares on 1049 close binaries. We identify 234 flare binaries, on which 6818 flares are detected. We compare the flare-binary fraction in different binary morphologies ("detachedness"). The result shows that the fractions in over-contact and ellipsoidal binaries are approximately 10-20 percent lower than those in detached and semi-detached systems. We calculate the binary flares activity level (AL) of all the flare binaries, and discuss its variations along the orbital period (P_orb) and rotation period (P_rot, calculated for only detached binaries). We find that AL increases with decreasing P_orb or P_rot up to the critical values at P_orb near 3 days or P_rot near 1.5 days, thereafter, the AL starts decreasing no matter how fast the stars rotate. We examine the flaring rate as a function of orbital phase in 2 eclipsing binaries on which a large number of flares are detected. It appears that there is no correlation between flaring rate ...

  4. Electronic band structures of binary skutterudites

    International Nuclear Information System (INIS)

    The electronic properties of complex binary skutterudites, MX3 (M = Co, Rh, Ir; X = P, As, Sb) are explored, using various density functional theory (DFT) based theoretical approaches including Green's Function (GW) as well as regular and non-regular Tran Blaha modified Becke Jhonson (TB-mBJ) methods. The wide range of calculated bandgap values for each compound of this skutterudites family confirm that they are theoretically as challenging as their experimental studies. The computationally expensive GW method, which is generally assume to be efficient in the reproduction of the experimental bandgaps, is also not very successful in the calculation of bandgaps. In this article, the issue of the theoretical bandgaps of these compounds is resolved by reproducing the accurate experimental bandgaps, using the recently developed non-regular TB-mBJ approach, based on DFT. The effectiveness of this technique is due to the fact that a large volume of the binary skutterudite crystal is empty and hence quite large proportion of electrons lie outside of the atomic spheres, where unlike LDA and GGA which are poor in the treatment of these electrons, this technique properly treats these electrons and hence reproduces the clear electronic picture of these compounds. - Highlights: • Theoretical and experimental electronic band structures of binary skutterudites are reviewed. • The literature reveals that none of the existing theoretical results are consistent with the experiments. • GW, regular and non-regular TB-mBJ methods are used to reproduce the correct results. • The GW and regular TB-mBJ results are better than the available results in literature. • However, non-regular TB-mBJ reproduces the correct experimental band structures

  5. Electronic band structures of binary skutterudites

    Energy Technology Data Exchange (ETDEWEB)

    Khan, Banaras [Center for Computational Materials Science, University of Malakand, Chakdara (Pakistan); Department of Physics, University of Malakand, Chakdara (Pakistan); Aliabad, H.A. Rahnamaye [Department of Physics, Hakim Sabzevari University, Sabzevar (Iran, Islamic Republic of); Saifullah [Center for Computational Materials Science, University of Malakand, Chakdara (Pakistan); Department of Physics, University of Malakand, Chakdara (Pakistan); Jalali-Asadabadi, S. [Department of Physics, Faculty of Science, University of Isfahan (UI), 81744 Isfahan (Iran, Islamic Republic of); Khan, Imad [Center for Computational Materials Science, University of Malakand, Chakdara (Pakistan); Department of Physics, University of Malakand, Chakdara (Pakistan); Ahmad, Iftikhar, E-mail: ahma5532@gmail.com [Center for Computational Materials Science, University of Malakand, Chakdara (Pakistan); Department of Physics, University of Malakand, Chakdara (Pakistan)

    2015-10-25

    The electronic properties of complex binary skutterudites, MX{sub 3} (M = Co, Rh, Ir; X = P, As, Sb) are explored, using various density functional theory (DFT) based theoretical approaches including Green's Function (GW) as well as regular and non-regular Tran Blaha modified Becke Jhonson (TB-mBJ) methods. The wide range of calculated bandgap values for each compound of this skutterudites family confirm that they are theoretically as challenging as their experimental studies. The computationally expensive GW method, which is generally assume to be efficient in the reproduction of the experimental bandgaps, is also not very successful in the calculation of bandgaps. In this article, the issue of the theoretical bandgaps of these compounds is resolved by reproducing the accurate experimental bandgaps, using the recently developed non-regular TB-mBJ approach, based on DFT. The effectiveness of this technique is due to the fact that a large volume of the binary skutterudite crystal is empty and hence quite large proportion of electrons lie outside of the atomic spheres, where unlike LDA and GGA which are poor in the treatment of these electrons, this technique properly treats these electrons and hence reproduces the clear electronic picture of these compounds. - Highlights: • Theoretical and experimental electronic band structures of binary skutterudites are reviewed. • The literature reveals that none of the existing theoretical results are consistent with the experiments. • GW, regular and non-regular TB-mBJ methods are used to reproduce the correct results. • The GW and regular TB-mBJ results are better than the available results in literature. • However, non-regular TB-mBJ reproduces the correct experimental band structures.

  6. Observational signatures of binary supermassive black holes

    Energy Technology Data Exchange (ETDEWEB)

    Roedig, Constanze; Krolik, Julian H. [Department of Physics and Astronomy, Johns Hopkins University, Baltimore, MD 21218 (United States); Miller, M. Coleman [Department of Astronomy and Joint Space-Science Institute, University of Maryland, College Park, MD 20742 (United States)

    2014-04-20

    Observations indicate that most massive galaxies contain a supermassive black hole, and theoretical studies suggest that when such galaxies have a major merger, the central black holes will form a binary and eventually coalesce. Here we discuss two spectral signatures of such binaries that may help distinguish them from ordinary active galactic nuclei. These signatures are expected when the mass ratio between the holes is not extreme and the system is fed by a circumbinary disk. One such signature is a notch in the thermal continuum that has been predicted by other authors; we point out that it should be accompanied by a spectral revival at shorter wavelengths and also discuss its dependence on binary properties such as mass, mass ratio, and separation. In particular, we note that the wavelength λ {sub n} at which the notch occurs depends on these three parameters in such a way as to make the number of systems displaying these notches ∝λ{sub n}{sup 16/3}; longer wavelength searches are therefore strongly favored. A second signature, first discussed here, is hard X-ray emission with a Wien-like spectrum at a characteristic temperature ∼100 keV produced by Compton cooling of the shock generated when streams from the circumbinary disk hit the accretion disks around the individual black holes. We investigate the observability of both signatures. The hard X-ray signal may be particularly valuable as it can provide an indicator of black hole merger a few decades in advance of the event.

  7. Anonymizing Binary Tables is APX-hard

    CERN Document Server

    Bonizzoni, Paola; Dondi, Riccardo

    2007-01-01

    The problem of publishing personal data without giving up privacy is increasingly important. An interesting formalization is the $k$-anonymization, where all rows in a table are clustered in sets of at least $k$ records, and all the entries for which records in the same cluster have different values are suppressed. The problem has been shown to be NP-hard when the records values are over a ternary alphabet and $k=3$. In this paper we show that the problem is not only NP-hard, but also APX-hard, when the records values are over a binary alphabet and $k=3$.

  8. Astronomical Plate Archives and Binary Blazars Studies

    Czech Academy of Sciences Publication Activity Database

    Hudec, René

    2011-01-01

    Roč. 32, 1-2 (2011), s. 91-95. ISSN 0250-6335. [Conference on Multiwavelength Variability of Blazars. Guangzhou, 22,09,2010-24,09,2010] R&D Projects: GA ČR GA205/08/1207 Grant ostatní: GA ČR(CZ) GA102/09/0997; MŠMT(CZ) ME09027 Institutional research plan: CEZ:AV0Z10030501 Keywords : astronomical plates * plate archives archives * binary blazars Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 0.400, year: 2011

  9. Statistical kinetic treatment of relativistic binary collisions.

    Science.gov (United States)

    Peano, F; Marti, M; Silva, L O; Coppa, G

    2009-02-01

    In particle-based algorithms, the effect of binary collisions is commonly described in a statistical way, using Monte Carlo techniques. It is shown that, in the relativistic regime, stringent constraints should be considered on the sampling of particle pairs for collision, which are critical to ensure physically meaningful results, and that nonrelativistic sampling criteria (e.g., uniform random pairing) yield qualitatively wrong results, including equilibrium distributions that differ from the theoretical Jüttner distribution. A general procedure for relativistically consistent algorithms is provided, and verified with three-dimensional Monte Carlo simulations, thus opening the way to the numerical exploration of the statistical properties of collisional relativistic systems. PMID:19391799

  10. 6384 Kervin: A Possible Hungaria Binary Asteroid

    Science.gov (United States)

    Warner, Brian D.; Aznar Macia, Amadeo

    2016-04-01

    Analysis of CCD photometric observations in late 2015 of the Hungaria asteroid 6384 Kervin indicates that it may be a binary asteroid with a primary lightcurve of P1 = 3.6194 ± 0.0001 h, A1 = 0.06 ± 0.01 mag. The secondary lightcurve parameters are P2 = 15.94 ± 0.01 h, A2 = 0.03 ± 0.01 mag. No mutual events (occultations or eclipses) were observed. However, other indicators give an estimated diameter ratio on the order of Ds/Dp ~ 0.3, possibly greater.

  11. Associative memory - An optimum binary neuron representation

    Science.gov (United States)

    Awwal, A. A.; Karim, M. A.; Liu, H. K.

    1989-01-01

    Convergence mechanism of vectors in the Hopfield's neural network is studied in terms of both weights (i.e., inner products) and Hamming distance. It is shown that Hamming distance should not always be used in determining the convergence of vectors. Instead, weights (which in turn depend on the neuron representation) are found to play a more dominant role in the convergence mechanism. Consequently, a new binary neuron representation for associative memory is proposed. With the new neuron representation, the associative memory responds unambiguously to the partial input in retrieving the stored information.

  12. X-ray binaries, part 1

    International Nuclear Information System (INIS)

    Optical observations of X-ray binaries and their interpretation are described. A number of early-type stars which are identified as companions of X-ray sources are photometrically and spectroscopically observed. The spectra were obtained with the coude spectrograph attached to the 1.5 m telescope of the European Southern Observatory, La Silla, Chile. Registrations of the spectra were made with the Faul-Coradi microphotometer of the Observatory at Utrecht. To study radial velocity variations, the positions of the spectral lines were measured with the Grant comparator of the University of Groningen

  13. Model for magnetic-nonmagnetic binary alloys

    Energy Technology Data Exchange (ETDEWEB)

    Razafimandimby, H. [Departement de Physique, Universite de Toliara, 601 Toliara (Madagascar); Randrianasoloharisoa, D. [LPMR, Universite d' Antananarivo (Madagascar); Rakotomahevitra, A. [Departement des Sciences Exactes, Universite de Mahajanga, BP 155 (Madagascar); Parlebas, J.C. [IPCMS, UMR 7504 CNRS-Universite Louis Pasteur, 23 rue du Loess, BP 43, 67034 Strasbourg (France)

    2007-10-15

    An extension of a mean-field approximation (MFA) developed within standard basis operators (SBO) is used to study magnetism in magnetic-nonmagnetic binary alloys. The Curie temperature is calculated from the free energy within the framework of the present approach. The calculated results are in fair agreement with the theoretical results of other research groups for the same problem but utilizing other methods. Finally, the case of NiPt alloys is briefly examined as an example test for the comparison with experiment. (copyright 2007 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  14. Is the Coma cluster binary dominated?

    International Nuclear Information System (INIS)

    It is investigated whether the model of an expanding cluster dominated by a massive binary galaxy, first suggested by Valtonen and Byrd (1979), is consistent with optical data on the surface density and velocity dispersion of the Coma cluster. The evolution of this model is simulated for a wide variety of initial conditions. It is found that galaxy counts in the model can be made to agree with observation, but that the observed velocity dispersion profile cannot be reproduced. A number of other arguments suggest that the central galaxies in Coma cannot be as massive as required by the model. This model is not a viable representation of the Coma cluster. 25 refs

  15. Double riches: asteroseismology in eclipsing binaries

    CERN Document Server

    Southworth, John

    2015-01-01

    The study of eclipsing binaries is our primary source of measured properties of normal stars, achieved through analysis of light and radial velocity curves of eclipsing systems. The study of oscillations and pulsations is increasingly vital for determining the properties of single stars, and investigating the physical phenomena active in their interiors. Combining the two methods holds the promise of establishing stringent tests of stellar evolutionary theory, and of calibrating model-dependent asteroseismology with empirically measured stellar properties. I review recent advances and outline future work.

  16. NONLINEAR TIDES IN CLOSE BINARY SYSTEMS

    International Nuclear Information System (INIS)

    We study the excitation and damping of tides in close binary systems, accounting for the leading-order nonlinear corrections to linear tidal theory. These nonlinear corrections include two distinct physical effects: three-mode nonlinear interactions, i.e., the redistribution of energy among stellar modes of oscillation, and nonlinear excitation of stellar normal modes by the time-varying gravitational potential of the companion. This paper, the first in a series, presents the formalism for studying nonlinear tides and studies the nonlinear stability of the linear tidal flow. Although the formalism we present is applicable to binaries containing stars, planets, and/or compact objects, we focus on non-rotating solar-type stars with stellar or planetary companions. Our primary results include the following: (1) The linear tidal solution almost universally used in studies of binary evolution is unstable over much of the parameter space in which it is employed. More specifically, resonantly excited internal gravity waves in solar-type stars are nonlinearly unstable to parametric resonance for companion masses M' ∼> 10-100 M⊕ at orbital periods P ≈ 1-10 days. The nearly static 'equilibrium' tidal distortion is, however, stable to parametric resonance except for solar binaries with P ∼3[P/10 days] for a solar-type star) and drives them as a single coherent unit with growth rates that are a factor of ≈N faster than the standard three-wave parametric instability. These are local instabilities viewed through the lens of global analysis; the coherent global growth rate follows local rates in the regions where the shear is strongest. In solar-type stars, the dynamical tide is unstable to this collective version of the parametric instability for even sub-Jupiter companion masses with P ∼< a month. (4) Independent of the parametric instability, the dynamical and equilibrium tides excite a wide range of stellar p-modes and g-modes by nonlinear inhomogeneous forcing

  17. Nonlinear Tides in Close Binary Systems

    Science.gov (United States)

    Weinberg, Nevin N.; Arras, Phil; Quataert, Eliot; Burkart, Josh

    2012-06-01

    We study the excitation and damping of tides in close binary systems, accounting for the leading-order nonlinear corrections to linear tidal theory. These nonlinear corrections include two distinct physical effects: three-mode nonlinear interactions, i.e., the redistribution of energy among stellar modes of oscillation, and nonlinear excitation of stellar normal modes by the time-varying gravitational potential of the companion. This paper, the first in a series, presents the formalism for studying nonlinear tides and studies the nonlinear stability of the linear tidal flow. Although the formalism we present is applicable to binaries containing stars, planets, and/or compact objects, we focus on non-rotating solar-type stars with stellar or planetary companions. Our primary results include the following: (1) The linear tidal solution almost universally used in studies of binary evolution is unstable over much of the parameter space in which it is employed. More specifically, resonantly excited internal gravity waves in solar-type stars are nonlinearly unstable to parametric resonance for companion masses M' >~ 10-100 M ⊕ at orbital periods P ≈ 1-10 days. The nearly static "equilibrium" tidal distortion is, however, stable to parametric resonance except for solar binaries with P companion masses larger than a few Jupiter masses, the dynamical tide causes short length scale waves to grow so rapidly that they must be treated as traveling waves, rather than standing waves. (3) We show that the global three-wave treatment of parametric instability typically used in the astrophysics literature does not yield the fastest-growing daughter modes or instability threshold in many cases. We find a form of parametric instability in which a single parent wave excites a very large number of daughter waves (N ≈ 103[P/10 days] for a solar-type star) and drives them as a single coherent unit with growth rates that are a factor of ≈N faster than the standard three

  18. Computer Vision Using Local Binary Patterns

    CERN Document Server

    Pietikainen, Matti; Zhao, Guoying; Ahonen, Timo

    2011-01-01

    The recent emergence of Local Binary Patterns (LBP) has led to significant progress in applying texture methods to various computer vision problems and applications. The focus of this research has broadened from 2D textures to 3D textures and spatiotemporal (dynamic) textures. Also, where texture was once utilized for applications such as remote sensing, industrial inspection and biomedical image analysis, the introduction of LBP-based approaches have provided outstanding results in problems relating to face and activity analysis, with future scope for face and facial expression recognition, b

  19. Stretchable Binary Fresnel Lens for Focus Tuning.

    Science.gov (United States)

    Li, Xueming; Wei, Lei; Poelma, René H; Vollebregt, Sten; Wei, Jia; Urbach, Hendrik Paul; Sarro, Pasqualina M; Zhang, Guo Qi

    2016-01-01

    This paper presents a tuneable binary amplitude Fresnel lens produced by wafer-level microfabrication. The Fresnel lens is fabricated by encapsulating lithographically defined vertically aligned carbon nanotube (CNT) bundles inside a polydimethyl-siloxane (PDMS) layer. The composite lens material combines the excellent optical absorption properties of the CNT with the transparency and stretchability of the PDMS. By stretching the elastomeric composite in radial direction, the lens focal length is tuned. Good focusing response is demonstrated and a large focus change (≥24%) was achieved by stretching lenses up to 11.4%. PMID:27139747

  20. Physical Structure of Four Symbiotic Binaries

    Science.gov (United States)

    Kenyon, Scott J. (Principal Investigator)

    1997-01-01

    Disk accretion powers many astronomical objects, including pre-main sequence stars, interacting binary systems, and active galactic nuclei. Unfortunately, models developed to explain the behavior of disks and their surroundings - boundary layers, jets, and winds - lack much predictive power, because the physical mechanism driving disk evolution - the viscosity - is not understood. Observations of many types of accreting systems are needed to constrain the basic physics of disks and provide input for improved models. Symbiotic stars are an attractive laboratory for studying physical phenomena associated with disk accretion. These long period binaries (P(sub orb) approx. 2-3 yr) contain an evolved red giant star, a hot companion, and an ionized nebula. The secondary star usually is a white dwarf accreting material from the wind of its red giant companion. A good example of this type of symbiotic is BF Cygni: our analysis shows that disk accretion powers the nuclear burning shell of the hot white dwarf and also manages to eject material perpendicular to the orbital plane (Mikolajewska, Kenyon, and Mikolajewski 1989). The hot components in other symbiotic binaries appear powered by tidal overflow from a very evolved red giant companion. We recently completed a study of CI Cygni and demonstrated that the accreting secondary is a solar-type main sequence star, rather than a white dwarf (Kenyon et aL 1991). This project continued our study of symbiotic binary systems. Our general plan was to combine archival ultraviolet and optical spectrophotometry with high quality optical radial velocity observations to determine the variation of line and continuum sources as functions of orbital phase. We were very successful in generating orbital solutions and phasing UV+optical spectra for five systems: AG Dra, V443 Her, RW Hya, AG Peg, and AX Per. Summaries of our main results for these systems appear below. A second goal of our project was to consider general models for the

  1. Binary magnetic structures in HoEr

    DEFF Research Database (Denmark)

    Howard, B.K.; Bohr, J.

    1991-01-01

    The magnetic structure of a single crystal of the rare earth random alloy Ho50% Er50% has been investigated by elastic neutron diffraction measurements in the temperature range 120-10 K. Three distinct magnetic phases are identified below the Neel temperature of 104 K. The high-temperature phase...... observed between 104 K and 47.5 K is a binary magnetic structure where the holmium and erbium moments belong to different modulated c-axis spirals. The intermediate-temperature phase between 47.5 K and 35 K is a simple basal plane spiral. Below 35 K, the measurements suggest a ferrimagnetic structure in...

  2. Binary quadratic forms an algorithmic approach

    CERN Document Server

    Buchmann, Johannes

    2007-01-01

    The book deals with algorithmic problems related to binary quadratic forms, such as finding the representations of an integer by a form with integer coefficients, finding the minimum of a form with real coefficients and deciding equivalence of two forms. In order to solve those problems, the book introduces the reader to important areas of number theory such as diophantine equations, reduction theory of quadratic forms, geometry of numbers and algebraic number theory. The book explains applications to cryptography. It requires only basic mathematical knowledge.

  3. Binary Colloidal Alloy Test-5: Phase Separation

    Science.gov (United States)

    Lynch, Matthew; Weitz, David A.; Lu, Peter J.

    2008-01-01

    The Binary Colloidal Alloy Test - 5: Phase Separation (BCAT-5-PhaseSep) experiment will photograph initially randomized colloidal samples onboard the ISS to determine their resulting structure over time. This allows the scientists to capture the kinetics (evolution) of their samples, as well as the final equilibrium state of each sample. BCAT-5-PhaseSep studies collapse (phase separation rates that impact product shelf-life); in microgravity the physics of collapse is not masked by being reduced to a simple top and bottom phase as it is on Earth.

  4. Multiple Human Tracking Using Binary Infrared Sensors

    Directory of Open Access Journals (Sweden)

    Toshiaki Miyazaki

    2015-06-01

    Full Text Available To create a context-aware environment, human locations and movement paths must be considered. In this paper, we propose an algorithm that tracks human movement paths using only binary sensed data obtained by infrared (IR sensors attached to the ceiling of a room. Our algorithm can estimate multiple human movement paths without a priori knowledge of the number of humans in the room. By repeating predictions and estimations of human positions and links from the previous human positions to the estimated ones at each time period, human movement paths can be estimated. Simulation-based evaluation results show that our algorithm can dynamically trace human movement paths.

  5. On Totally Reducible Binary Forms: I

    Indian Academy of Sciences (India)

    C Hooley

    2001-08-01

    Let () be the number of positive numbers up to a large limit that are expressible in essentially more than one way by a binary form that is a product of > 2 distinct linear factors with integral coefficients. We prove that $$(n) = O\\left(n^{2/l-_l+\\epsilon}\\right),$$ where \\begin{equation*}_l=\\begin{cases}1/l^2, \\quad\\text{if}\\quad l=3,\\\\ (l-2)/l^2(l-1), \\quad\\text{if}\\quad l>3,\\end{cases}\\end{equation*} thus demonstrating in particular that it is exceptional for a number represented by to have essentially more than one representation.

  6. Binary Nucleation of Water and Sodium Chloride

    Energy Technology Data Exchange (ETDEWEB)

    Nemec, Thomas [Institute of Thermomechanics ASCR, Prague, Czech Republic; Marsik, Frantisek [Institute of Thermomechanics ASCR, Prague, Czech Republic; Palmer, Donald [ORNL

    2005-01-01

    Nucleation processes in the binary water-sodium chloride system are investigated in the sense of the classical nucleation theory (CNT). The CNT is modified to be able to handle the electrolytic nature of the system and is employed to investigate the acceleration of the nucleation process due to the presence of sodium chloride in the steam. This phenomenon, frequently observed in the Wilson zone of steam turbines, is called early condensation. Therefore, the nucleation rates of the water-sodium chloride mixture are of key importance in the power cycle industry.

  7. HD 161306: a radiatively interacting Be binary?

    Czech Academy of Sciences Publication Activity Database

    Koubský, Pavel; Kotková, Lenka; Kraus, Michaela; Yang, S.; Šlechta, Miroslav; Harmanec, P.; Wolf, M.; Votruba, Viktor; Kubát, Jiří; Kubátová, Brankica; Niemczura, E.; Škoda, Petr

    2014-01-01

    Roč. 567, July (2014), A57/1-A57/4. ISSN 0004-6361 R&D Projects: GA ČR(CZ) GA14-21373S; GA MŠk LG14026 Grant ostatní: ESA(XE) ESA-PECS project no. 98058; GA ČR(CZ) GAP209/10/0715 Institutional support: RVO:67985815 Keywords : binaries: spectroscopic * stars: emission-line * Be: stars Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 4.378, year: 2014

  8. The Gaia Mission, Binary Stars and Exoplanets

    CERN Document Server

    Eyer, Laurent; Holl, Berry; North, Pierre; Zucker, Shay; Evans, Dafydd W; Pourbaix, Dimitri; Hodgkin, Simon T; Thuillot, William; Mowlavi, Nami; Carry, Benoit

    2015-01-01

    On the 19th of December 2013, the Gaia spacecraft was successfully launched by a Soyuz rocket from French Guiana and started its amazing journey to map and characterise one billion celestial objects with its one billion pixel camera. In this presentation, we briefly review the general aims of the mission and describe what has happened since launch, including the Ecliptic Pole scanning mode. We also focus especially on binary stars, starting with some basic observational aspects, and then turning to the remarkable harvest that Gaia is expected to yield for these objects.

  9. Iterative Method for Generating Correlated Binary Sequences

    CERN Document Server

    Usatenko, O V; Apostolov, S S; Makarov, N M; Krokhin, A A

    2014-01-01

    We propose a new efficient iterative method for generating random correlated binary sequences with prescribed correlation function. The method is based on consecutive linear modulations of initially uncorrelated sequence into a correlated one. Each step of modulation increases the correlations until the desired level has been reached. Robustness and efficiency for the proposed algorithm are tested by generating sequences with inverse power-law correlations. The substantial increase in the strength of correlation in the iterative method with respect to the single-step filtering generation is shown for all studied correlation functions. Our results can be used for design of disordered superlattices, waveguides, and surfaces with selective transport properties.

  10. UV Emission line shifts of symbiotic binaries

    OpenAIRE

    Friedjung, M.; Mikolajewska, J.; Zajczyk, A.; Eriksson, M.

    2010-01-01

    Relative and absolute emission line shifts have been previously found for symbiotic binaries, but their cause was not clear. This work aims to better understand the emission line shifts. Positions of strong emission lines were measured on archival UV spectra of Z And, AG Dra, RW Hya, SY Mus and AX Per and relative shifts between the lines of different ions compared. Profiles of lines of RW Hya and Z And were also examined. The reality of the relative shift between resonance and intercombinati...

  11. Retrograde binaries of massive black holes in circum-binary accretion discs

    CERN Document Server

    Amaro-Seoane, Pau; Dotti, Massimo; Colpi, Monica

    2016-01-01

    We explore the hardening of a massive black hole binary embedded in a circum-binary gas disc when the binary and the gas are coplanar and the gas is counter-rotating. The secondary black hole, revolving in the direction opposite to the gas, experiences a drag from gas-dynamical friction and from direct accretion of part of it. Using two-dimensional (2D) hydrodynamical grid simulations we investigate the effect of changing the accretion prescriptions on the dynamics of the secondary black hole which in turn affect the binary hardening and eccentricity evolution. We find that realistic accretion prescriptions lead to results that differ from those inferred assuming accretion of all the gas within the Roche Lobe of the secondary black hole. Different accretion prescriptions result in different disc's surface densities which alter the black hole's dynamics back. Full 3D SPH realizations of a number of representative cases, run over a shorter interval of time, validate the general trends observed in the less compu...

  12. Merging binary black holes formed through chemically homogeneous evolution in short-period stellar binaries

    Science.gov (United States)

    Mandel, Ilya; de Mink, Selma E.

    2016-05-01

    We explore a newly proposed channel to create binary black holes of stellar origin. This scenario applies to massive, tight binaries where mixing induced by rotation and tides transports the products of hydrogen burning throughout the stellar envelopes. This slowly enriches the entire star with helium, preventing the build-up of an internal chemical gradient. The stars remain compact as they evolve nearly chemically homogeneously, eventually forming two black holes, which we estimate typically merge 4-11 Gyr after formation. Like other proposed channels, this evolutionary pathway suffers from significant theoretical uncertainties, but could be constrained in the near future by data from advanced ground-based gravitational-wave detectors. We perform Monte Carlo simulations of the expected merger rate over cosmic time to explore the implications and uncertainties. Our default model for this channel yields a local binary black hole merger rate of about 10 Gpc-3 yr-1 at redshift z = 0, peaking at twice this rate at z = 0.5. This means that this channel is competitive, in terms of expected rates, with the conventional formation scenarios that involve a common-envelope phase during isolated binary evolution or dynamical interaction in a dense cluster. The events from this channel may be distinguished by the preference for nearly equal-mass components and high masses, with typical total masses between 50 and 110 M⊙. Unlike the conventional isolated binary evolution scenario that involves shrinkage of the orbit during a common-envelope phase, short time delays are unlikely for this channel, implying that we do not expect mergers at high redshift.

  13. Kepler Eclipsing Binary Stars. VIII. Identification of False Positive Eclipsing Binaries and Re-extraction of New Light Curves

    Science.gov (United States)

    Abdul-Masih, Michael; Prša, Andrej; Conroy, Kyle; Bloemen, Steven; Boyajian, Tabetha; Doyle, Laurance R.; Johnston, Cole; Kostov, Veselin; Latham, David W.; Matijevič, Gal; Shporer, Avi; Southworth, John

    2016-04-01

    The Kepler mission has provided unprecedented, nearly continuous photometric data of ∼200,000 objects in the ∼105 deg2 field of view (FOV) from the beginning of science operations in May of 2009 until the loss of the second reaction wheel in May of 2013. The Kepler Eclipsing Binary Catalog contains information including but not limited to ephemerides, stellar parameters, and analytical approximation fits for every known eclipsing binary system in the Kepler FOV. Using target pixel level data collected from Kepler in conjunction with the Kepler Eclipsing Binary Catalog, we identify false positives among eclipsing binaries, i.e., targets that are not eclipsing binaries themselves, but are instead contaminated by eclipsing binary sources nearby on the sky and show eclipsing binary signatures in their light curves. We present methods for identifying these false positives and for extracting new light curves for the true source of the observed binary signal. For each source, we extract three separate light curves for each quarter of available data by optimizing the signal-to-noise ratio, the relative percent eclipse depth, and the flux eclipse depth. We present 289 new eclipsing binaries in the Kepler FOV that were not targets for observation, and these have been added to the catalog. An online version of this catalog with downloadable content and visualization tools is maintained at http://keplerEBs.villanova.edu.

  14. A Compact Supermassive Binary Black Hole System

    CERN Document Server

    Rodríguez, C; Zavala, R T; Peck, A B; Pollack, L K; Romani, R W

    2006-01-01

    We report on the discovery of a supermassive binary black hole system in the radio galaxy 0402+379, with a projected separation between the two black holes of just 7.3 pc. This is the closest black hole pair yet found by more than two orders of magnitude. These results are based upon recent multi-frequency observations using the Very Long Baseline Array (VLBA) which reveal two compact, variable, flat-spectrum, active nuclei within the elliptical host galaxy of 0402+379. Multi-epoch observations from the VLBA also provide constraints on the total mass and dynamics of the system. Low spectral resolution spectroscopy using the Hobby-Eberly Telescope indicates two velocity systems with a combined mass of the two black holes of ~1.5 x 10^8 solar masses. The two nuclei appear stationary while the jets emanating from the weaker of the two nuclei appear to move out and terminate in bright hot spots. The discovery of this system has implications for the number of close binary black holes that might be sources of gravi...

  15. Observations of accretion discs in interacting binaries

    Science.gov (United States)

    Honey, William Bruce

    Cataclysmic and X-ray binaries (CV and LMXB) are considered, and new observations of both types of source are considered. Chapter 1 gives an introduction to the subject and presents a study of the evolution and period relationships of these objects. Chapter 2 studies the superoutburst of a system. The observational data presented in the Chapter are used to place constraints on the geometry of the system, and also upon the theoretical models examined; only eccentric disc models are found to be acceptable. A tidally dominated eccentric accretion disc is considered, and good agreement between the observations and a tidally distorted disc simulation is achieved. In Chapter 3, a search for the superhump phenomenon is conducted. No such superhump behavior was found. These observations support the ideas first raised in Chapter 2 of the importance of tidal behavior in dwarf novae. Chapter 4 reviews observations of black hole candidates, and lists the generally expected 'fingerprint' thought to be associated with black holes in binary systems. Chapter 5 reports on observations of the LMXB GX339-4 and the discovery of the period for the system. Constraints on the system parameters are given and a model is presented that is compatible with the observations. Chapter 6 reviews the work done and considers other important observational evidence that is to be found in the literature and is pertinent to the work in this thesis.

  16. Multilevel Cross-Dependent Binary Longitudinal Data

    KAUST Repository

    Serban, Nicoleta

    2013-10-16

    We provide insights into new methodology for the analysis of multilevel binary data observed longitudinally, when the repeated longitudinal measurements are correlated. The proposed model is logistic functional regression conditioned on three latent processes describing the within- and between-variability, and describing the cross-dependence of the repeated longitudinal measurements. We estimate the model components without employing mixed-effects modeling but assuming an approximation to the logistic link function. The primary objectives of this article are to highlight the challenges in the estimation of the model components, to compare two approximations to the logistic regression function, linear and exponential, and to discuss their advantages and limitations. The linear approximation is computationally efficient whereas the exponential approximation applies for rare events functional data. Our methods are inspired by and applied to a scientific experiment on spectral backscatter from long range infrared light detection and ranging (LIDAR) data. The models are general and relevant to many new binary functional data sets, with or without dependence between repeated functional measurements.

  17. Gravitational redshift from a binary system

    Energy Technology Data Exchange (ETDEWEB)

    Steklain, Andre [Universidade Tecnologica Federal do Parana (UTFPR), PR (Brazil)

    2011-07-01

    Full text: In this work we study the gravitational redshift of a binary system in general relativity. We employ a mixed metric obtained from the matching of a 1PN metric with two perturbed Schwarzschild metrics, based on previous works [Alvi, Phys. Rev. D, 61, 124013 (2000)]. This metric is well known, and has been considered for several applications [Steklain et al, Phys. Lett. A, 373, 188, (2009)]. We consider a massless observer in a timelike geodesic of this metric measuring the redshift of the system. The observer concentrates the redshift measurements in one of the massive bodies and is influenced by the mass of the second body. We find that there is a substantial contribution of the second mass in some cases. We compare with experimental data obtained for real binary systems of white dwarfs [Vennes et al, Astroph. J., L37 (1991)]. We also discuss these results for more massive systems, and make some predictions for very massive systems, like black holes, although it extrapolates the limit of the 1PN approximation used. Is well known that the major contribution of the observed redshift is from the universe expansion, but these results indicate that the influence of the gravitational redshift may be underestimated at some systems. (author)

  18. Tertiary companions to close spectroscopic binaries

    CERN Document Server

    Tokovinin, A; Thomas, S; Udry, S

    2006-01-01

    We have surveyed a sample of 165 solar-type spectroscopic binaries (SB) with periods from 1 to 30 days for higher-order multiplicity. 62 targets have been observed with the NACO adaptive optics system and 13 new physical tertiary companions were detected. Another 12 new wide companions (5 still tentative) were retrieved from the 2MASS sky survey. Our binaries belong to 161 stellar systems; of these 64 are triple, 11 quadruple and 7 quintuple. After correction for incomplete detection, the fraction of SBs with additional companions is 63% +- 5%. We find that this fraction is a strong function of the SB period P, reaching 96% for P12d. Period distributions of SBs with and without tertiaries are significantly different, but their mass ratio distributions are identical. New statistical data on the multiplicity of close SBs indicate that their periods and mass ratios were established very early, but periods of SBs within triples were further shortened by angular momentum exchange with companions.

  19. Transient High Mass X-ray Binaries

    CERN Document Server

    Paul, Biswajit

    2011-01-01

    High Mass X-ray Binaries (HMXBs) are interesting objects that provide a wide range of observational probes to the nature of the two stellar components, accretion process, stellar wind and orbital parameters of the systems. A large fraction of the transient HMXBs are found to be Be/X-ray binaries in which the companion Be star with its circumstellar disk governs the outburst. These outbursts are understood to be due to the sudden enhanced mass accretion to the neutron star and is likely to be associated with changes in the circumstellar disk of the companion. In the recent years, another class of transient HMXBs have been found which have supergiant companions and show shorter bursts. X-ray, infrared and optical observations of these objects provide vital information regarding these systems. Here we review some key observational properties of the transient HMXBs and also discuss some important recent developments from studies of this class of sources. The X-ray properties of these objects are discussed in some...

  20. Thirty New Low-Mass Spectroscopic Binaries

    CERN Document Server

    Shkolnik, Evgenya L; Liu, Michael C; Reid, I Neill; Cameron, Andrew C

    2010-01-01

    As part of our search for young M dwarfs within 25 pc, we acquired high-resolution spectra of 185 low-mass stars compiled by the NStars project that have strong X-ray emission. By cross-correlating these spectra with radial velocity standard stars, we are sensitive to finding multi-lined spectroscopic binaries. We find a low-mass spectroscopic binary fraction of 16% consisting of 27 SB2s, 2 SB3s and 1 SB4, increasing the number of known low-mass SBs by 50% and proving that strong X-ray emission is an extremely efficient way to find M-dwarf SBs. WASP photometry of 23 of these systems revealed two low-mass EBs, bringing the count of known M dwarf EBs to 15. BD -22 5866, the SB4, is fully described in Shkolnik et al. 2008 and CCDM J04404+3127 B consists of a two mid-M stars orbiting each other every 2.048 days. WASP also provided rotation periods for 12 systems, and in the cases where the synchronization time scales are short, we used P_rot to determine the true orbital parameters. For those with no P_rot, we us...

  1. Speakers' choice of frame in binary choice

    Directory of Open Access Journals (Sweden)

    Marc van Buiten

    2009-02-01

    Full Text Available A distinction is proposed between extit{recommending for} preferred choice options and extit{recommending against} non-preferred choice options. In binary choice, both recommendation modes are logically, though not psychologically, equivalent. We report empirical evidence showing that speakers recommending for preferred options predominantly select positive frames, which are less common when speakers recommend against non-preferred options. In addition, option attractiveness is shown to affect speakers' choice of frame, and adoption of recommendation mode. The results are interpreted in terms of three compatibility effects, (i extit{recommendation mode---valence framing compatibility}: speakers' preference for positive framing is enhanced under extit{recommending for} and diminished under extit{recommending against} instructions, (ii extit{option attractiveness---valence framing compatibility}: speakers' preference for positive framing is more pronounced for attractive than for unattractive options, and (iii extit{recommendation mode---option attractiveness compatibility}: speakers are more likely to adopt a extit{recommending for} approach for attractive than for unattractive binary choice pairs.

  2. Ordering in binary transition metal alloys

    Energy Technology Data Exchange (ETDEWEB)

    Rusakov, G. [Institute for Metal Physics UB RAS, 18 Kovalevskoj St., 620990 Ekaterinburg (Russian Federation); Ural State Technical University - UPI, 19 Mira St., 620002 Ekaterinburg (Russian Federation); Son, L., E-mail: ldson@yandex.ru [Ural State Pedagogical University, 26 Cosmonavtov Ave, 620017 Ekaterinburg (Russian Federation); Efimova, E. [Institute for Metal Physics UB RAS, 18 Kovalevskoj St., 620990 Ekaterinburg (Russian Federation); Ural State Technical University - UPI, 19 Mira St., 620002 Ekaterinburg (Russian Federation); Dubinin, N. [Institute for Metallurgy UB RAS, 101 Amundsen St., 620016 Ekaterinburg (Russian Federation); Ural State Technical University - UPI, 19 Mira St., 620002 Ekaterinburg (Russian Federation)

    2012-03-20

    We present the phenomenological thermodynamic modeling of binary alloys which demonstrate solubility of the components at high temperatures, and form intermediate phase near equiatomic composition at lower ones (the so-called sigma-phase). Besides, the regular solution miscibility gap takes place also. The nonequilibrium thermodynamic potential is written out as a sum of the free energy of regular solution and polynomial term of scalar order parameter {phi}, which describes the {sigma}-phase ordering. There are four parameters in the model: the energy of regular solution mixing, the energy of {sigma}-phase formation at zero temperature, and the widths of temperature and concentration intervals of {sigma}-phase existence in the alloy with frozen-in random distribution of components. Up to now, both phase transitions which take place in a number of transition metals binary alloys (the {sigma}-phase formation and miscibility in the regular solution) have been treated separately. In present work, the standard technique of phase diagram calculation allows us to analyze all possible phase diagrams which may arise in the alloy.

  3. Convergence Speed of Binary Interval Consensus

    CERN Document Server

    Draief, Moez

    2012-01-01

    We consider the convergence time for solving the binary consensus problem using the interval consensus algorithm proposed by B\\' en\\' ezit, Thiran and Vetterli (2009). In the binary consensus problem, each node initially holds one of two states and the goal for each node is to correctly decide which one of these two states was initially held by a majority of nodes. We derive an upper bound on the expected convergence time that holds for arbitrary connected graphs, which is based on the location of eigenvalues of some contact rate matrices. We instantiate our bound for particular networks of interest, including complete graphs, paths, cycles, star-shaped networks, and Erd\\" os-R\\' enyi random graphs; for these graphs, we compare our bound with alternative computations. We find that for all these examples our bound is tight, yielding the exact order with respect to the number of nodes. We pinpoint the fact that the expected convergence time critically depends on the voting margin defined as the difference betwe...

  4. Modeling and analysis of advanced binary cycles

    Energy Technology Data Exchange (ETDEWEB)

    Gawlik, K.

    1997-12-31

    A computer model (Cycle Analysis Simulation Tool, CAST) and a methodology have been developed to perform value analysis for small, low- to moderate-temperature binary geothermal power plants. The value analysis method allows for incremental changes in the levelized electricity cost (LEC) to be determined between a baseline plant and a modified plant. Thermodynamic cycle analyses and component sizing are carried out in the model followed by economic analysis which provides LEC results. The emphasis of the present work is on evaluating the effect of mixed working fluids instead of pure fluids on the LEC of a geothermal binary plant that uses a simple Organic Rankine Cycle. Four resources were studied spanning the range of 265{degrees}F to 375{degrees}F. A variety of isobutane and propane based mixtures, in addition to pure fluids, were used as working fluids. This study shows that the use of propane mixtures at a 265{degrees}F resource can reduce the LEC by 24% when compared to a base case value that utilizes commercial isobutane as its working fluid. The cost savings drop to 6% for a 375{degrees}F resource, where an isobutane mixture is favored. Supercritical cycles were found to have the lowest cost at all resources.

  5. The binary-rich cluster Abell 2244

    International Nuclear Information System (INIS)

    A structural, luminosity, and velocity study of the cluster Abell 2244 is presented. A2244 was selected for study from the list of Struble and Rood because of its unusually binary-rich population in an evolved cD-type cluster environment, a contradiction in terms of cluster collapse scenarios versus the formation and survival of bound pairs. In comparison with the Coma cluster, A2244 is a slightly poorer version with a suspected deficiency in the number of cluster members in the core. This evidence, combined with the high-velocity dispersion of the cluster, suggests that a low-velocity population of galaxies has been consumed by the central cD galaxy. The central cD galaxy has a single low-velocity companion deep in its envelope and cross sections of its surface-brightness profile suggest that it is in transition from a depressed central surface-brightness object to a high central concentration system, a point of contention in merger models. All but two of the binary galaxies were found to be projections with other cluster members or stars and, thus, are not in conflict with the advanced dynamical age of the cluster. 35 refs

  6. The Electromagnetic Signals of Compact Binary Mergers

    CERN Document Server

    Piran, T; Rosswog, S

    2012-01-01

    Compact binary mergers are prime sources of gravitational waves (GWs), targeted by current and next generation detectors. The question "what is the observable electromagnetic (EM) signature of a compact binary merger?" is an intriguing one with crucial consequences to the quest for gravitational waves. We present a large set of numerical simulations that focus on the electromagnetic signals that emerge from the dynamically ejected sub-relativistic material. These outflows produce on a time scale of a day macronovae - short-lived optical/UV signals powered by radioactive decay. In addition, the outflow interaction with the surrounding matter inevitably leads to a long-lasting radio emission. We calculate the expected radio signals from these outflows on time scales longer than a year, when the sub-relativistic ejecta dominate the emission. We discuss their detectability in 1.4 GHz and 150 MHz and compare it with an updated estimate of the detectability of short GRBs' orphan afterglows. We find that mergers wit...

  7. ACOUSTIC EFFECTS ON BINARY AEROELASTICITY MODEL

    Directory of Open Access Journals (Sweden)

    Kok Hwa Yu

    2011-10-01

    Full Text Available Acoustics is the science concerned with the study of sound. The effects of sound on structures attract overwhelm interests and numerous studies were carried out in this particular area. Many of the preliminary investigations show that acoustic pressure produces significant influences on structures such as thin plate, membrane and also high-impedance medium like water (and other similar fluids. Thus, it is useful to investigate the structure response with the presence of acoustics on aircraft, especially on aircraft wings, tails and control surfaces which are vulnerable to flutter phenomena. The present paper describes the modeling of structural-acoustic interactions to simulate the external acoustic effect on binary flutter model. Here, the binary flutter model which illustrated as a rectangular wing is constructed using strip theory with simplified unsteady aerodynamics involving flap and pitch degree of freedom terms. The external acoustic excitation, on the other hand, is modeled using four-node quadrilateral isoparametric element via finite element approach. Both equations then carefully coupled and solved using eigenvalue solution. The mentioned approach is implemented in MATLAB and the outcome of the simulated result are later described, analyzed and illustrated in this paper.

  8. A Speeding Binary in the Galactic Halo

    Science.gov (United States)

    Kohler, Susanna

    2016-04-01

    The recent discovery of a hyper-velocity binary star system in the halo of the Milky Way poses a mystery: how was this system accelerated to its high speed?Accelerating StarsUnlike the uniform motion in the Galactic disk, stars in the Milky Ways halo exhibit a huge diversity of orbits that are usually tilted relative to the disk and have a variety of speeds. One type of halo star, so-called hyper-velocity stars, travel with speeds that can approach the escape velocity of the Galaxy.How do these hyper-velocity stars come about? Assuming they form in the Galactic disk, there are multiple proposed scenarios through which they could be accelerated and injected into the halo, such as:Ejection after a close encounter with the supermassive black hole at the Galactic centerEjection due to a nearby supernova explosionEjection as the result of a dynamical interaction in a dense stellar population.Further observations of hyper-velocity stars are necessary to identify the mechanism responsible for their acceleration.J1211s SurpriseModels of J1211s orbit show it did not originate from the Galactic center (black dot). The solar symbol shows the position of the Sun and the star shows the current position of J1211. The bottom two panels show two depictions(x-y plane and r-z plane) of estimated orbits of J1211 over the past 10 Gyr. [Nmeth et al. 2016]To this end, a team of scientists led by Pter Nmeth (Friedrich Alexander University, Erlangen-Nrnberg) recently studied the candidate halo hyper-velocity star SDSS J121150.27+143716.2. The scientists obtained spectroscopy of J1211 using spectrographs at the Keck Telescope in Hawaii and ESOs Very Large Telescope in Chile. To their surprise, they discovered the signature of a companion in the spectra: J1211 is actually a binary!Nmeth and collaborators found that J1211, located roughly 18,000 light-years away, is moving at a rapid ~570 km/s relative to the galactic rest frame. The binary system consists of a hot (30,600 K) subdwarf and a

  9. Observations of Binary and Millisecond Pulsars at Xinjiang Astronomical Observatory

    Indian Academy of Sciences (India)

    Jingbo Wang; Na Wang; Jianping Yuan; Zhiyong Liu

    2014-09-01

    We present the first results of radio timing observations of binary and millisecond pulsars in China. We have timed four binary pulsars for 9 years, using Nanshan 25-m radio telescope. The long time span has enabled us to determine their rotation and orbital parameters.

  10. Optical studies of massive X-ray binaries

    International Nuclear Information System (INIS)

    Photometric and spectroscopic studies of several optical counterparts of massive X-ray binaries are presented. Subjects of study were the binary systems:HD77581/4U0900-40 (Vela X-1), HD153919/4U1700-37, Wray 977/4U1223-62 and Sk160/4U0115-74 (=SMC X-1). (Auth.)

  11. The state of globular clusters at birth II: primordial binaries

    CERN Document Server

    Leigh, Nathan W C; Marks, Michael; Webb, Jeremy J; Hypki, Arkadiusz; Heinke, Craig O; Kroupa, Pavel; Sills, Alison

    2014-01-01

    (abridged) In this paper, we constrain the properties of primordial binary populations in Galactic globular clusters using the MOCCA Monte Carlo code for cluster evolution. Our results are compared to the observations of Milone et al. (2012) using the photometric binary populations as proxies for the true underlying distributions, in order to test the hypothesis that the data are consistent with an universal initial binary fraction near unity and the binary orbital parameter distributions of Kroupa (1995). With the exception of a few possible outliers, we find that the data are to first-order consistent with the universality hypothesis. Specifically, the present-day binary fractions inside the half-mass radius r$_{\\rm h}$ can be reproduced assuming either high initial binary fractions near unity with a dominant soft binary component as in the Kroupa distribution combined with high initial densities (10$^4$-10$^6$ M$_{\\odot}$ pc$^{-3}$), or low initial binary fractions ($\\sim$ 5-10%) with a dominant hard binar...

  12. Orbital Circularization of Hot and Cool Kepler Eclipsing Binaries

    DEFF Research Database (Denmark)

    Van Eylen, Vincent; Winn, Joshua N.; Albrecht, Simon

    2016-01-01

    . Here we seek evidence for the predicted dependence of circularization upon stellar type, using a sample of 945 eclipsing binaries observed by Kepler. This sample complements earlier studies of this effect, which employed smaller samples of better-characterized stars. For each Kepler binary we measure...

  13. The angular velocity of the apsidal rotation in binary stars

    CERN Document Server

    Vasilev, B V

    2004-01-01

    The shape of a rotating star consisting of equilibrium plasma is considered. The velocity of apsidal rotation of close binary stars (periastron rotation) which depends on the star shapes is calculated. The obtained estimations are in a good agreement with the observation data of the apsidal motion in binary systems.

  14. White dwarf-red dwarf binaries in the Galaxy

    NARCIS (Netherlands)

    Besselaar, E.J.M. van den

    2007-01-01

    This PhD thesis shows several studies on white dwarf - red dwarf binaries. White dwarfs are the end products of most stars and red dwarfs are normal hydrogen burning low-mass stars. White dwarf - red dwarf binaries are both blue (white dwarf) and red (red dwarf). Together with the fact that they are

  15. Grammar-Based Specification and Parsing of Binary File Formats

    Directory of Open Access Journals (Sweden)

    William Underwood

    2012-03-01

    Full Text Available The capability to validate and view or play binary file formats, as well as to convert binary file formats to standard or current file formats, is critically important to the preservation of digital data and records. This paper describes the extension of context-free grammars from strings to binary files. Binary files are arrays of data types, such as long and short integers, floating-point numbers and pointers, as well as characters. The concept of an attribute grammar is extended to these context-free array grammars. This attribute grammar has been used to define a number of chunk-based and directory-based binary file formats. A parser generator has been used with some of these grammars to generate syntax checkers (recognizers for validating binary file formats. Among the potential benefits of an attribute grammar-based approach to specification and parsing of binary file formats is that attribute grammars not only support format validation, but support generation of error messages during validation of format, validation of semantic constraints, attribute value extraction (characterization, generation of viewers or players for file formats, and conversion to current or standard file formats. The significance of these results is that with these extensions to core computer science concepts, traditional parser/compiler technologies can potentially be used as a part of a general, cost effective curation strategy for binary file formats.

  16. An eccentric binary millisecond pulsar in the Galactic plane

    NARCIS (Netherlands)

    D.J. Champion; S.M. Ransom; P. Lazarus; F. Camilo; C. Bassa; V.M. Kaspi; D.J. Nice; P.C.C. Freire; I.H. Stairs; J. van Leeuwen; B.W. Stappers; J.M. Cordes; J.W.T. Hessels; D.R. Lorimer; Z. Arzoumanian; D.C. Backer; N.D.R. Bhat; S. Chatterjee; I. Cognard; J.S. Deneva; C.A. Faucher-Giguère; B.M. Gaensler; J. Han; F.A. Jenet; L. Kasian; V.I. Kondratiev; M. Kramer; J. Lazio; M.A. McLaughlin; A. Venkataraman; W. Vlemmings

    2008-01-01

    Binary pulsar systems are superb probes of stellar and binary evolution and the physics of extreme environments. In a survey with the Arecibo telescope, we have found PSR J1903+ 0327, a radio pulsar with a rotational period of 2.15 milliseconds in a highly eccentric ( e = 0.44) 95- day orbit around

  17. Milankovitch Cycles of Terrestrial Planets in Binary Star Systems

    Science.gov (United States)

    Forgan, Duncan

    2016-08-01

    The habitability of planets in binary star systems depends not only on the radiation environment created by the two stars, but also on the perturbations to planetary orbits and rotation produced by the gravitational field of the binary and neighbouring planets. Habitable planets in binaries may therefore experience significant perturbations in orbit and spin. The direct effects of orbital resonances and secular evolution on the climate of binary planets remain largely unconsidered. We present latitudinal energy balance modelling of exoplanet climates with direct coupling to an N Body integrator and an obliquity evolution model. This allows us to simultaneously investigate the thermal and dynamical evolution of planets orbiting binary stars, and discover gravito-climatic oscillations on dynamical and secular timescales. We investigate the Kepler-47 and Alpha Centauri systems as archetypes of P and S type binary systems respectively. In the first case, Earthlike planets would experience rapid Milankovitch cycles (of order 1000 years) in eccentricity, obliquity and precession, inducing temperature oscillations of similar periods (modulated by other planets in the system). These secular temperature variations have amplitudes similar to those induced on the much shorter timescale of the binary period. In the Alpha Centauri system, the influence of the secondary produces eccentricity variations on 15,000 year timescales. This produces climate oscillations of similar strength to the variation on the orbital timescale of the binary. Phase drifts between eccentricity and obliquity oscillations creates further cycles that are of order 100,000 years in duration, which are further modulated by neighbouring planets.

  18. Single-, two-, and three-phase binary-alloy systems

    Science.gov (United States)

    Tenney, D. R.

    1980-01-01

    Series of three computer programs solves one-dimensional transient diffusion problems in single-and multiphase binary-alloy systems. Accurate understanding of diffusion process in binary-alloy system is important for development of metal matrix composites, some protective coatings, and thin-film technology.

  19. IUE observations of the eclipsing binary Epsilon Aurigae

    International Nuclear Information System (INIS)

    It is stated that the eclipsing binary Epsilon Aur is a most peculiar binary system and it has not been explained satisfactorily. Observations of this system using the International Ultraviolet Explorer (IUE) collected at the Villafranca Satellite Tracking Station of the European Space Agency are here reported. (author)

  20. Binary and ternary fission within the statistical model

    International Nuclear Information System (INIS)

    The binary and ternary nuclear fission are treated within the statistical model. At the scission point we calculate the potentials as functions of the deformations of the fragments in the dinuclear model. The potentials give the mass and charge distributions of the fission fragments. The ternary fission is assumed to occur during the binary fission. (author)