Sample records for beta spectroscopy

  1. Simultaneous beta and gamma spectroscopy (United States)

    Farsoni, Abdollah T.; Hamby, David M.


    A phoswich radiation detector for simultaneous spectroscopy of beta rays and gamma rays includes three scintillators with different decay time characteristics. Two of the three scintillators are used for beta detection and the third scintillator is used for gamma detection. A pulse induced by an interaction of radiation with the detector is digitally analyzed to classify the type of event as beta, gamma, or unknown. A pulse is classified as a beta event if the pulse originated from just the first scintillator alone or from just the first and the second scintillator. A pulse from just the third scintillator is recorded as gamma event. Other pulses are rejected as unknown events.

  2. In-trap decay spectroscopy for {beta}{beta} decays

    Energy Technology Data Exchange (ETDEWEB)

    Brunner, Thomas


    The presented work describes the implementation of a new technique to measure electron-capture (EC) branching ratios (BRs) of intermediate nuclei in {beta}{beta} decays. This technique has been developed at TRIUMF in Vancouver, Canada. It facilitates one of TRIUMF's Ion Traps for Atomic and Nuclear science (TITAN), the Electron Beam Ion Trap (EBIT) that is used as a spectroscopy Penning trap. Radioactive ions, produced at the radioactive isotope facility ISAC, are injected and stored in the spectroscopy Penning trap while their decays are observed. A key feature of this technique is the use of a strong magnetic field, required for trapping. It radially confines electrons from {beta} decays along the trap axis while X-rays, following an EC, are emitted isotropically. This provides spatial separation of X-ray and {beta} detection with almost no {beta}-induced background at the X-ray detector, allowing weak EC branches to be measured. Furthermore, the combination of several traps allows one to isobarically clean the sample prior to the in-trap decay spectroscopy measurement. This technique has been developed to measure ECBRs of transition nuclei in {beta}{beta} decays. Detailed knowledge of these electron capture branches is crucial for a better understanding of the underlying nuclear physics in {beta}{beta} decays. These branches are typically of the order of 10{sup -5} and therefore difficult to measure. Conventional measurements suffer from isobaric contamination and a dominating {beta} background at theX-ray detector. Additionally, X-rays are attenuated by the material where the radioactive sample is implanted. To overcome these limitations, the technique of in-trap decay spectroscopy has been developed. In this work, the EBIT was connected to the TITAN beam line and has been commissioned. Using the developed beam diagnostics, ions were injected into the Penning trap and systematic studies on injection and storage optimization were performed. Furthermore, Ge

  3. Scintillation spectroscopy for beta ray dose measurements

    Energy Technology Data Exchange (ETDEWEB)

    Vapirev, E.I.; Jordanov, T.; Amin, S.; Stoilov, N.; Georgieva, K. [Sofia Univ. (Bulgaria). Fizicheski Fakultet


    Two methods have been developed and tested for the measurement of beta ray dose with a scintillation probe. According to the first method the energy absorbed in plastic filters is calculated from the difference between the energy E of the incident and filtered beta spectrum with an expression of the type E {approx} c{Sigma}iN(i)/{Delta}m, where c is a calibration constant (keV per channel), i is the channel number, N(i) is the detected beta spectrum, and {Delta}m is the filter thickness. According to the second `dE/dx` method the energy deposited in the surface layer of the scintillator is calculated by E {approx} c{Sigma}dE/dx(i)N(i), where dE/dx is the specific energy loss for tissue-equivalent media. The methods were tested for the cases of normally incident electrons and surface contamination. The scintillation probe used is stillbene and the test sources are thin {sup 90}Sr/{sup 90}Y and {sup 137}Cs. The results are close to the expected doses as calculated by Monte Carlo simulations. (Author).

  4. Beta-delayed neutron spectroscopy using ion traps (United States)

    Wang, Barbara; Czeszumska, A.; Siegl, K.; Caldwell, S.; Aprahamian, A.; Burkey, M.; Clark, J.; Levand, A.; Marley, S.; Morgan, G.; Norman, E.; Nystrom, A.; Orford, R.; Padgett, S.; Perez Galvan, A.; Savard, G.; Scielzo, N.; Sharma, K.; Strauss, S.


    Trapped radioactive ions suspended in vacuum allow for a new way to perform beta-delayed neutron spectroscopy. Decay branching ratios and energy spectra of the emitted neutrons are inferred from a measurement of the nuclear recoil, thereby circumventing the many limitations associated with direct neutron detection. Beta-delayed neutron measurements were carried out for 137-138,140I, 134-136Sb, and 144-145Cs at the Californium Rare Isotope Breeder Upgrade (CARIBU) facility at Argonne National Laboratory. The data collected are needed in many fields of basic and applied science such as nuclear energy, nuclear astrophysics, and stockpile stewardship. Results for the isotopes 135-136Sb and 140I will be presented. Supported by NSF under PHY-1419765, and U.S. DOE under NEUP 13-5485, DE-AC02-06CH11357 (ANL), DE-AC52-07NA27344 (LLNL), and DE-NA0000979 (NNSA).

  5. Spectroscopy with {beta}2p and {beta}-{nu} recoil shifts

    Energy Technology Data Exchange (ETDEWEB)

    Fynbo, H.O.U. E-mail:; Axelsson, L.; Aeystoe, J.; Bergmann, U.C.; Borge, M.J.G.; Fraile, L.M.; Honkanen, A.; Hornshoej, P.; Jading, Y.; Jokinen, A.; Jonson, B.; Martel, I.; Mukha, I.; Nilsson, T.; Nyman, G.; Oinonen, M.; Riisager, K.; Siiskonen, T.; Smedberg, M.H.; Thaysen, J.; Tengblad, O.; Wenander, F


    The beta-delayed proton emission from the lightest Ar-isotopes has been measured with a high-granularity, large solid-angle Si-detector setup. Although designed for the detection of beta-delayed two-proton and three-proton events, the setup also permitted measurement of proton energy shifts due to the beta-neutrino recoil. We discuss how spectroscopic information can be extracted from such measurements, even at the drip line. For the case of {sup 31}Ar, the ground-state spin could be determined as 5/2.

  6. Resonance Raman Spectroscopy of Beta-Carotene and Lycopene: A Physical Chemistry Experiment. (United States)

    Hoskins, L. C.


    Discusses the theory of resonance Raman (RR) spectroscopy as it applies to beta-carotene and lycopene pigments (found in tomatoes and carrots, respectively). Also discusses an experiment which demonstrates the theoretical principles involved. The experiment has been tested over a three-year period and has received excellent acceptance by physical…

  7. $\\beta$-delayed fission, laser spectroscopy and shape-coexistence studies with radioactive At beams

    CERN Multimedia

    We propose to study the $\\beta$-delayed fission, laser spectroscopy and radioactive decay of the newly available pure beams of neutron-deficient and neutron-rich astatine (Z=85) isotopes. The fission probability and the fission fragment distribution of the even-even isotopes $^{194,196}$Po following the $\\beta$-decay of the isotopes $^{194,196}$At will be studied with the Windmill setup. In-source laser spectroscopy will be performed on the entire astatine isotopic chain, using a combination of the Windmill setup, ISOLTRAP MR-ToF and ISOLDE Faraday. Radioactive decay data will be acquired at the Windmill setup throughout those studies and contribute to the global understanding of the phenomenon of shape coexistence in the neutron-deficient lead region.

  8. Measurement of Moments and Radii of Light Nuclei by Collinear Fast-Beam Laser Spectroscopy and $\\beta$-NMR Spectroscopy

    CERN Document Server

    Marinova, K P


    Nuclear Moments and radii of light unstable isotopes are investigated by applying different high-sensitivity and high-resolution techniques based on collinear fast-beam laser spectroscopy. A study of nuclear structure in the sd shell is performed on neon isotopes in the extended chain of $^{17-28}$Ne, in particular on the proton-halo candidate $^{17}$Ne. Measurements of hyperfine structure and isotope shift have become possible by introducing an ultra-sensitive non-optical detection method which is based on optical pumping, state-selective collisional ionization and $\\beta$-activity counting. The small effect of nuclear radii on the optical isotope shifts of light elements requires very accurate measurements. The errors are dominated by uncertainties of the Doppler shifts which are conventionally determined from precisely measured acceleration voltages. These uncertainties are removed by measuring the beam energy with simultaneous excitation of two optical lines in parallel / antiparallel beam configuration. ...

  9. Ground-state properties of K-isotopes from laser and $\\beta$-NMR spectroscopy

    CERN Multimedia

    Lievens, P; Rajabali, M M; Krieger, A R

    By combining high-resolution laser spectroscopy with $\\beta$-NMR spectroscopy on polarized K-beams we aim to establish the ground-state spins and magnetic moments of the neutron-rich $^{48,49,50,51}$K isotopes from N=29 to N=32. Spins and magnetic moments of the odd-K isotopes up to N=28 reveal an inversion of the ground-state, from the normal $\\,{I}$=3/2 ($\\pi{d}_{3/2}^{-1}$) in $^{41-45}$K$\\to\\,{I}$=1/2 ($\\pi{s}_{1/2}^{-1}$) in $^{47}$K. This inversion of the proton single particle levels is related to the strong proton $d_{3/2}$ - neutron $f_{7/2}$ interaction which lowers the energy of the $\\pi{d}_{3/2}$ single particle state when filling the $\

  10. Trapped-ion decay spectroscopy towards the determination of ground-state components of double-beta decay matrix elements

    CERN Document Server

    Brunner, T; Andreoiu, C; Brodeur, M; Delheji, P; Ettenauer, S; Frekers, D; Gallant, A T; Gernhäuser, R; Grossheim, A; Krücken, R; Lennarz, A; Lunney, D; Mücher, D; Ringle, R; Simon, M C; Simon, V V; Sjue, S K L; Zuber, K; Dilling, J


    A new technique has been developed at TRIUMF's TITAN facility to perform in-trap decay spectroscopy. The aim of this technique is to eventually measure weak electron capture branching ratios (ECBRs) and by this to consequently determine GT matrix elements of $\\beta\\beta$ decaying nuclei. These branching ratios provide important input to the theoretical description of these decays. The feasibility and power of the technique is demonstrated by measuring the ECBR of $^{124}$Cs.

  11. Ultrafast optical responses of {beta}-carotene and lycopene probed by sub-20-fs time-resolved coherent spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Fujiwara, M.; Sugisaki, M. [CREST-JST and Department of Physics, Osaka City University, Osaka 558-8585 (Japan); Gall, A.; Robert, B. [CEA, Institut de Biologie et Technologies de Saclay, and CNRS, Gif-sur-Yvette F-91191 (France); Cogdell, R.J. [IBLS, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow G12 8QQ, Scotland (United Kingdom); Hashimoto, H., E-mail: [CREST-JST and Department of Physics, Osaka City University, Osaka 558-8585 (Japan)


    We investigate how structural distortions in carotenoid cause decoherences of its high-frequency vibrational modes by applying the sub-20-fs time-resolved transient grating spectroscopy to {beta}-carotene and lycopene. The results indicate that the C=C central stretching mode shows significant loss of coherence under the effects of the steric hindrance between {beta}-ionone ring and polyene backbone, whereas the other high-frequency modes do not show such dependency on the structural distortions.

  12. Study on the complexation of isoquercitrin with {beta}-cyclodextrin and its derivatives by spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Wang Yunlong; Qiao Xiaona; Li Wenchao; Zhou Yehong; Jiao Yong [Research Center of Environmental Science and Engineering, School of Chemistry and Chemical Engineering, Shanxi University, Wu Cheng Road 36, Taiyuan 030006 (China); Yang Cheng [Department of Applied Chemistry, Osaka University, Suita 565-0871 (Japan); Dong Chuan, E-mail: [Research Center of Environmental Science and Engineering, School of Chemistry and Chemical Engineering, Shanxi University, Wu Cheng Road 36, Taiyuan 030006 (China); Inoue, Yoshihisa [Department of Applied Chemistry, Osaka University, Suita 565-0871 (Japan); Shuang, Shaomin, E-mail: [Research Center of Environmental Science and Engineering, School of Chemistry and Chemical Engineering, Shanxi University, Wu Cheng Road 36, Taiyuan 030006 (China)


    The inclusion complexes of isoquercitrin (IQ) with cyclodextrins (CDs) including {beta}-cyclodextrin ({beta}-CD), hydroxypropyl-{beta}-cyclodextrin (HP-{beta}-CD) and dimethyl-{beta}-cyclodextrin (DM-{beta}-CD) have been investigated using the methods of steady-state fluorescence, UV-vis absorption and induced circular dichroism. The stoichiometric ratio of the three complexes was found to be 1:1 and the stability constants (K) were estimated from spectrofluorometric titrations, as well as the thermodynamic parameters. Maximum inclusion ability was measured in the case of DM-{beta}-CD due to the increased hydrophobicity of the host cavity, followed by HP-{beta}-CD and {beta}-CD. The effect of pH on the complexation process was also quantitatively assessed. IQ exists in different molecular forms depending on pH and {beta}-CDs were most suitable for inclusion of the neutral form of IQ. The phase-solubility diagrams obtained with {beta}-CD, HP-{beta}-CD and DM-{beta}-CD were all classical A{sub L} type. And DM-{beta}-CD provided the best solubility enhancement, 12.3-fold increase compared to 2.8- and 7.5-fold increase for {beta}-CD and HP-{beta}-CD. The apparent stability constants obtained from the solubility data at 25 deg. C were comparable with those obtained from the fluorescence assays. Moreover, {sup 1}H NMR was carried out, which revealed that the IQ favorably inserted into the inner cavity from the chromone part instead of the phenyl part, which was in agreement with molecular modeling studies.

  13. Application of nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and kinetic modeling for elucidation of adsorption chemistry in uptake of tetracycline by zeolite beta. (United States)

    Kang, Jin; Liu, Huijuan; Zheng, Yu-Ming; Qu, Jiuhui; Chen, J Paul


    Extensive usage of tetracycline has resulted in its contamination in surface water and groundwater. The adsorption of tetracycline on zeolite beta was systematically investigated for the decontamination of the antibiotic polluted water in this study. Ninety percent of uptake by the zeolite beta occured in 0.25h, and the adsorption equilibrium was obtained within 3h, which was well described by an intraparticle diffusion model. The adsorption generally increased when pH was increased from 4.0 to 5.0, and then decreased significantly as the pH was further increased, which was caused by the pH-dependent speciation of tetracycline and surface charge of zeolite beta. Both Freundlich and Langmuir equations well described the adsorption isotherm. A thermodynamic analysis showed that the sorption process was spontaneous and endothermic. Aluminum atoms in the zeolite played a crucial role in the uptake; the adsorption increased with the increasing aluminum content in zeolite. The UV-Visible spectroscopy study showed that the spectra of tetracycline changed upon the interaction with zeolite beta, which could be ascribed to the formation of complexes of tetracycline and aluminum atoms in the zeolite surface. Nuclear magnetic resonance spectroscopy study further confirmed the participation of Al in the tetracycline adsorption. Fourier transform infrared spectroscopy studies showed that the amino functional groups in tetracycline were involved in the complexation with the zeolite surface.

  14. Total Absorption Gamma-Ray Spectroscopy of 87Br, 88Br and 94Rb Beta-Delayed Neutron Emitters

    CERN Document Server

    Valencia, E; Algora, A; Agramunt, J; Rubio, B; Rice, S; Gelletly, W; Regan, P; Zakari-Issoufou, A -A; Fallot, M; Porta, A; Rissanen, J; Eronen, T; Aysto, J; Batist, L; Bowry, M; Bui, V M; Caballero-Folch, R; Cano-Ott, D; Elomaa, V -V; Estevez, E; Farrelly, G F; Garcia, A R; Gomez-Hornillos, B; Gorlychev, V; Hakala, J; Jordan, M D; Jokinen, A; Kolhinen, V S; Kondev, F G; Martinez, T; Mendoza, E; Moore, I; Penttila, H; Podolyak, Zs; Reponen, M; Sonnenschein, V; Sonzogni, A A


    We investigate the decay of 87Br, 88Br and 94Rb using total absorption gamma-ray spectroscopy. These important fission products are beta-delayed neutron emitters. Our data show considerable gamma-intensity, so far unobserved in high-resolution gamma-ray spectroscopy, from states at high excitation energy. We also find significant differences with the beta intensity that can be deduced from existing measurements of the beta spectrum. We evaluate the impact of the present data on reactor decay heat using summation calculations. Although the effect is relatively small it helps to reduce the discrepancy between calculations and integral measurements of the photon component for 235U fission at cooling times in the range 1 to 100 s. We also use summation calculations to evaluate the impact of present data on reactor antineutrino spectra. We find a significant effect at antineutrino energies in the range of 5 to 9 MeV. In addition, we observe an unexpected strong probability for gamma emission from neutron unbound s...

  15. Dielectric spectroscopy in benzophenone: the beta relaxation and its relation to the mode-coupling Cole-Cole peak. (United States)

    Pardo, L C; Lunkenheimer, P; Loidl, A


    We report a thorough characterization of the glassy dynamics of benzophenone by broadband dielectric spectroscopy. We detect a well-pronounced beta relaxation peak developing into an excess wing with increasing temperature. A previous analysis of results from Optical-Kerr-effect measurements of this material within the mode-coupling theory revealed a high-frequency Cole-Cole peak. We address the question if this phenomenon also may explain the Johari-Goldstein beta relaxation, a so-far unexplained spectral feature inherent to glass-forming matter, mainly observed in dielectric spectra. Our results demonstrate that according to the present status of theory, both spectral features seem not to be directly related.

  16. Development of a portable triple silicon detector telescope for beta spectroscopy and skin dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    Helt-Hansen, J


    It is now recognized that beta radiation can be a significant radiation problem for exposure of the skin. There is thus a need for a portable and rugged active beta dosemeter-spectrometer to carry out immediate measurements of doses and energies of beta particles even in the presence of photon radiation. The main objective of this report is to describe the development of such an instrument. A beta-spectrometer has been developed consisting of three silicon surface barrier detectors with the thickness: 50{mu}m/150{mu}m/7000{mu}m covered by a 2 {mu}m thick titanium window. The spectrometer is capable of measuring electron energies from 50 keV to 3.5 MeV. The spectrometer is characterized by a compact low weight design, achieved by digital signal processing beginning at an early stage in the signal chain. 255 channels are available for each of the three detectors. The spectrometer is controlled by a laptop computer, which also handles all subsequent data analysis. By use of coincidence/anti-coincidence considerations of the absorbed energy in the three detector elements, counts caused by electrons are separated from those originating from photons. The electron energy distribution is multiplied by a set of conversion coefficients to obtain the dose at 0.07 mm tissue. Monte Carlo calculations has been used to derive the conversion coefficients and to investigate the influence of noise and the design of detector assembly on the performance of the spectrometer. This report describes the development of the spectrometer and its mode of operation, followed by a description of the Monte Carlo calculations carried out to obtain the conversion coefficients. Finally is the capability of the telescope spectrometer to measure beta and photon spectra as well as beta dose rates in pure beta and mixed beta/photon radiation fields described. (au)

  17. Rapid and simultaneous determination of lycopene and beta-carotene contents in tomato juice by infrared spectroscopy. (United States)

    De Nardo, Thais; Shiroma-Kian, Cecilia; Halim, Yuwana; Francis, David; Rodriguez-Saona, Luis E


    The rapid quantification of lycopene and beta-carotene in tomato juices by attenuated total reflectance (ATR) infrared spectroscopy combined with multivariate analysis was evaluated. Two sample preparation methods were compared: a direct measurement of the tomato paste and an extraction method using hexane to isolate carotenoids. HPLC was used as the reference method. Cross-validated (leave-one-out) partial least-squares regression (PLSR) was used to create calibration models to predict these phytonutrient concentrations in blind test samples. The infrared spectra showed unique marker bands at 957 and 968 cm(-1) for lycopene and beta-carotene, respectively. Multivariate analysis of the infrared spectral data gave correlation coefficients (r values) of >0.9 between the ATR-IR predicted and HPLC reference values, and standard errors of cross-validation (SECV) of 0.5 and 0.04 mg/100 g of juice for lycopene and beta-carotene, respectively. ATR-IR could provide the tomato industry with a simple, rapid, and high-throughput technique for the determination of tomato quality.

  18. Anodic oxides on a beta type Nb-Ti alloy and their characterization by electrochemical impedance spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Woldemedhin, Michael Teka; Hassel, Achim Walter [Max Planck Institut fuer Eisenforschung GmbH, Duesseldorf (Germany); Institute for Chemical Technology of Inorganic Materials, Johannes Kepler University, Linz (Austria); Raabe, Dierk [Max Planck Institut fuer Eisenforschung GmbH, Duesseldorf (Germany)


    Anodic oxides were grown on the surface of an electropolished (Ti-30 at% Nb) beta-titanium ({beta}-Ti) alloy by cyclic voltammetry. The scan rate was 100 mV s{sup -1} between 0 and 8 V in increments of l V in an acetate buffer of pH 6.0. Electrochemical impedance spectroscopy was carried out right after each anodic oxide growth increment to study the electronic properties of the oxide/electrolyte interface in a wide frequency range from 100 kHz to 10 MHz with an AC perturbation voltage of 10 mV. A film formation factor of 2.4 nm V{sup -1} was found and a relative permittivity number (dielectric constant) of 42.4 was determined for the oxide film formed. Mott-Schottky analysis on a potentiostatically formed 7 nm thick oxide film was performed to assess the semiconducting properties of the mixed anodic oxide grown on the alloy. A flat band potential of -0.47 V (standard hydrogen electrode, SHE) was determined, connected to a donor density of 8.2 x 10{sup 17} cm{sup -3}. {beta}-Ti being highly isotropic in terms of mechanical properties should be superior to the stiffer {alpha}-Ti compound. Its application, however, requires a passivation behaviour comparable or better than {alpha}-Ti which in fact is found. (Abstract Copyright [2010], Wiley Periodicals, Inc.)

  19. Beta-delayed gamma and proton spectroscopy near the Z=N line

    Energy Technology Data Exchange (ETDEWEB)

    Kankainen, A.; Eronen, T.; Hager, U.; Hakala, J.; Huang, W.; Huikari, J.; Jokinen, A.; Kopecky, S.; Moore, I.; Nieminen, A.; Penttilae, H.; Rinta-Antila, S.; Wang, Y.; Aeystoe, J. [University of Jyvaeskylae, Department of Physics, P.O. Box 35, Jyvaeskylae (Finland); Eliseev, S.A. [Petersburg Nuclear Physics Inst. (Russian Federation); GSI, Darmstadt (Germany); Fox, S.P.; Jenkins, D. [University of York, Department of Physics, Heslington (United Kingdom); Novikov, Yu.N.; Vorobjev, G.K. [Petersburg Nuclear Physics Inst. (Russian Federation); St. Petersburg Univ. (Russian Federation); Popov, A.V.; Seliverstov, D.M. [Petersburg Nuclear Physics Institute, Petersburg (Russian Federation); Schatz, H. [Michigan State University, East Lansing, MI (United States)


    A series of beta decay experiments on nuclei near the Z=N line has been performed using the ISOL technique at the IGISOL facility in Jyvaeskylae and at ISOLDE, CERN. The decay properties of these neutron-deficient nuclei are important in astrophysics as well as in the studies of isospin symmetry. (orig.)

  20. Signatures of beta-sheet secondary structures in linear and two-dimensional infrared spectroscopy

    NARCIS (Netherlands)

    Cheatum, CM; Tokmakoff, A; Knoester, J


    Using idealized models for parallel and antiparallel beta sheets, we calculate the linear and two-dimensional infrared spectra of the amide I vibration as a function of size and secondary structure. The model assumes transition-dipole coupling between the amide I oscillators in the sheet and account

  1. Beta-delayed neutron spectroscopy of spherical and deformed neutron emitters with VANDLE (United States)

    King, Thomas; Gross, C. J.; Grzywacz, R. K.; Paulauskas, S. V.; Rykaczewski, K. P.; Stracener, D. W.,; Taylor, S. Z.; Vandle Collaboration


    For many neutron-rich isotopes, the main decay mode is through beta-delayed neutron and gamma emission. Neutron and gamma coincidences provide information necessary to extract the beta-strength distribution. These distributions are inputs to test nuclear models needed for r-process modeling. The detailed data on beta decay feeding to neutron-unbound states are used to calculate reactor decay heat and understand the antineutrino spectrum. A series of measurements with selective ion sources was performed at the On-Line Test Facility (OLTF) at Oak Ridge National Laboratory with the Versatile Array of Neutron Detectors at Low Energy (VANDLE). These experiments revisited decays of spherical and deformed isotopes produced in proton induced fission of 238U, which included beta delayed precursors of bromine, rubidium, cesium, and iodine. Unique data sets with neutron and gamma ray coincidences were collected. Achieving high coincidence efficiency required the addition of high-efficiency gamma-ray detectors consisting of 16 LaBr3 crystals (HAGRiD) and a large volume set of NaI detectors to VANDLE. Preliminary results will be presented. This research was sponsored by DOE under Contracts DE-FG52-08NA2855, DE-AC05-00OR22725 and DE-FG02-96ER40983.

  2. Estimating Vegetation Beta Diversity from Airborne Imaging Spectroscopy and Unsupervised Clustering

    Directory of Open Access Journals (Sweden)

    Claire A. Baldeck


    Full Text Available Airborne remote sensing has an important role to play in mapping and monitoring biodiversity over large spatial scales. Techniques for applying this technology to biodiversity mapping have focused on remote species identification of individual crowns; however, this requires collection of a large number of crowns to train a classifier, which may limit the usefulness of this approach in many study regions. Based on the premise that the spectral variation among sites is related to their ecological dissimilarity, we asked whether it is possible to estimate the beta diversity, or turnover in species composition, among sites without the use of training data. We evaluated alternative methods using simulated communities constructed from the spectra of field-identified tree and shrub crowns from an African savanna. A method based on the k-means clustering of crown spectra produced beta diversity estimates (measured as Bray-Curtis dissimilarity among sites with an average pairwise correlation of ~0.5 with the true beta diversity, compared to an average correlation of ~0.8 obtained by a supervised species classification approach. When applied to savanna landscapes, the unsupervised clustering method produced beta diversity estimates similar to those obtained from supervised classification. The unsupervised method proposed here can be used to estimate the spatial structure of species turnover in a landscape when training data (e.g., tree crowns are unavailable, providing top-down information for science, conservation and ecosystem management applications.

  3. Determination of lycopene and beta-carotene content in tomato fruits and related products: Comparison of FT-Raman, ATR-IR, and NIR spectroscopy. (United States)

    Baranska, M; Schütze, W; Schulz, H


    Tomatoes and various products derived from thermally processed tomatoes are major sources of lycopene, but apart from this micronutrient, other carotenoids such as beta-carotene also are present in the fruit. They occur in tomato fruits and various tomato products in amounts of 2.62-629.00 (lycopene) and 0.23-2.83 mg/100 g (beta-carotene). Standard methods for determining the carotenoid content require the extraction of the analyte as well as other cleanup steps. In this work, FT-Raman, ATR-IR, and NIR spectroscopy are applied in order to establish new, fast, and nondestructive calibration methods for quantification of lycopene and beta-carotene content in tomato fruits and related products. The best prediction quality was achieved using a model based on IR spectroscopy (R2 = 0.98 and 0.97, SECV = 33.20 and 0.16 for lycopene and beta-carotene, respectively). In spite of the fact that Raman spectra of tomato products show characteristic key bands of the investigated carotenoids, this method gives slightly lower reliability (R2 = 0.91 and 0.89, SECV = 74.34 and 0.34 for lycopene and beta-carotene, respectively). NIR spectroscopy, which has been used for quantification purposes in the agricultural sector for several decades, in this study shows the worse prediction quality (R2 = 0.85 and 0.80, SECV = 91.19 and 0.41 for lycopene and beta-carotene, respectively).

  4. Auger line shape and electron energy loss spectroscopy analysis of amorphous, microcrystalline, and. beta. -SiC

    Energy Technology Data Exchange (ETDEWEB)

    Nelson, A.J.; Mason, A.R.; Swartzlander, A.B.; Kazmerski, L.L. (Solar Energy Research Institute, Golden, CO (USA)); Saxena, N.; Fortmann, C.M.; Russell, T.W.F. (Institute of Energy Conversion, University of Delaware, Newark, DE (USA))


    Auger electron spectroscopy (AES) line shape analysis of the Si-{ital L}{sub 23}{ital VV} and C-{ital KLL} peaks has been performed in conjunction with electron energy loss spectroscopy (EELS) on Hg-sensitized photodeposited amorphous and microcyrstalline SiC films. Mixtures of SiH{sub 4}/CH{sub 3}SiH{sub 3} and SiH{sub 4}/(CH{sub 3}){sub 2}SiH{sub 2} with helium or hydrogen dilution were used for the depositions. AES line shape and EELS analyses were also performed on {beta}-SiC for comparison. Quantitative bulk compositional analysis to determine the Si and C concentrations in these films was performed with an electron microprobe (EMPA) using x-ray wavelength dispersive spectroscopy (WDS). AES and EELS results reveal the predominant Si--C bonding and relative crystallinity in the films as a function of deposition parameters, which includes the gas mixture, pressure, and H{sub 2}/He dilution. These parameters determine the H radical flux during growth, which leads to changes in the film structure.

  5. Prompt Beta Spectroscopy as a Diagnostic for Mix in Ignited NIF Capsules

    CERN Document Server

    Hayes, A C; Solem, J C; Bradley, P A; Rundberg, R S


    The National Ignition Facility (NIF) technology is designed to drive deuterium-tritium (DT) internal confinement fusion (ICF) targets to ignition using indirect radiation from laser beam energy captured in a hohlraum. Hydrodynamical instabilities at interfaces in the ICF capsule leading to mix between the DT fue l and the ablator shell material are of fundamental physical interest and can affect the performance characteristics of the capsule. In this Letter we describe new radiochemical diagnostics for mix processes in ICF capsules with plastic or Be (0.9%Cu) ablator shells. Reactions of high-energy tritons with shell material produce high-energy $\\beta$-emitters. We show that mix between the DT fuel and the shell material enhances high-energy prompt beta emission from these reactions by more than an order of magnitude over that expected in the absence of mix.

  6. Isomer and beta decay spectroscopy in the 132Sn region with EURICA

    Directory of Open Access Journals (Sweden)

    Jungclaus A.


    Full Text Available The first EURICA campaign with high intensity Uranium beams took place at RIKEN in November/December 2012. Within this campaign experiment NP1112-RIBF85 was performed dedicated to the study of the isomeric and beta decays of neutronrich Cd, In, Sn and Sb isotopes towards and beyond the N=82 neutron shell closure. In this contribution we present a first status report of the analysis of the extensive data set obtained in this experiment.

  7. Harmonic Force Spectroscopy Reveals a Force-Velocity Curve from a Single Human Beta Cardiac Myosin Motor

    DEFF Research Database (Denmark)

    Sung, Jongmin; Nag, Suman; Vestergaard, Christian L.;


    A muscle contracts rapidly under low load, but slowly under high load. This load-dependent muscle shortening has been described with a hyperbolic load-velocity curve. Its molecular mechanisms remain to be elucidated, however. During muscle contraction, myosins in thick filaments interact with actin...... is slow under high load and fast under low load. We use a new, simple method we call "harmonic force spectroscopy" to extract a load-velocity relationship from a single human beta cardiac myosin II motor (S1). With a dual-beam optical trap, we hold an actin dumbbell over a single myosin molecule...... that is anchored to the microscope stage, which we oscillate sinusoidally in the direction of the dumbbell. Upon binding of the motor to the actin filament, it experiences an oscillatory load with a mean value that may be directed forward or backward, depending on where the binding took place. We find...

  8. Rapid quantification of polycyclic aromatic hydrocarbons in hydroxypropyl-{beta}-cyclodextrin (HPCD) soil extracts by synchronous fluorescence spectroscopy (SFS)

    Energy Technology Data Exchange (ETDEWEB)

    Hua Guoxiong [School of Biology and Psychology, Institute for Research on Environment and Sustainability, Newcastle University, Newcastle upon Tyne NE1 7RU (United Kingdom)]. E-mail:; Broderick, John [School of Biology and Psychology, Institute for Research on Environment and Sustainability, Newcastle University, Newcastle upon Tyne NE1 7RU (United Kingdom); Semple, Kirk T. [Department of Environmental Science, Faculty of Science and Technology, University of Lancaster, Lancaster LA1 4YQ (United Kingdom); Killham, Ken [School of Biological Sciences, University of Aberdeen, Aberdeen AB24 3UU (United Kingdom); Singleton, Ian [School of Biology and Psychology, Institute for Research on Environment and Sustainability, Newcastle University, Newcastle upon Tyne NE1 7RU (United Kingdom)


    Synchronous fluorescence spectroscopy (SFS) was directly applied to rapidly quantify selected polycyclic aromatic hydrocarbons (PAHs: benzo[a]pyrene and pyrene) in aqueous hydroxypropyl-{beta}-cyclodextrin (HPCD) soil extract solutions from a variety of aged contaminated soils containing four different PAHs. The method was optimized and validated. The results show that SFS can be used to analyse benzo[a]pyrene and pyrene in HPCD based soil extracts with high sensitivity and selectivity. The linear calibration ranges were 4.0 x 10{sup -6}-1.0 x 10{sup -3} mM for benzo[a]pyrene and 6.0 x 10{sup -6}-1.2 x 10{sup -3} mM for pyrene in 10 mM HPCD aqueous solution alone. The detection limits according to the error propagation theory for benzo[a]pyrene and pyrene were 3.9 x 10{sup -6} and 5.4 x 10{sup -6} mM, respectively. A good agreement between SFS and HPLC was reached for both determinations of PAHs in HPCD alone and in soil HPCD extracts. Hence, SFS is a potential means to simplify the present non-exhaustive hydroxypropyl-{beta}-cyclodextrin (HPCD)-based extraction technique for the evaluation of PAH bioavailability in soil. - SFS can be used to rapidly quantify selected PAHs in soil extracts and to simplify the non-exhaustive HPCD-based extraction technique for the evaluation of PAH bioavailability.

  9. A kinetic study of electrochemical lithium insertion in nanosized rutile {beta}-MnO{sub 2} by impedance spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Bach, S., E-mail: [Institut de Chimie et des Materiaux Paris Est, GESMAT, UMR 7182 CNRS-Universite Paris XII, 2 rue Henri Dunant 94320 Thiais (France); Universite d' Evry Val d' Essonne, Bd F.Mitterrand, Departement Chimie, 91025 Evry Cedex (France); Pereira-Ramos, J.P. [Institut de Chimie et des Materiaux Paris Est, GESMAT, UMR 7182 CNRS-Universite Paris XII, 2 rue Henri Dunant 94320 Thiais (France); Willmann, P. [Centre National d' Etudes Spatiales, 118 avenue Edouard Belin, 31401 Toulouse Cedex 9 (France)


    The kinetics of the electrochemical lithium insertion reaction in nano-sized rutile {beta}-MnO{sub 2} has been investigated using ac impedance spectroscopy. The experimental kinetic data are obtained for a rutile compound synthesized by ball-milling the powder produced from the heat treatment of manganese nitrate salts. The results are discussed as a function of the Li content for 0 < x < 0.6 and the number of cycles in the 4.1-2 V window. From a comparison with data obtained on the micro-sized oxide, an improved kinetics is found with D{sub Li} values for the apparent chemical diffusion coefficient of lithium much higher by one order of magnitude than in microsized oxide. Impedance behaviour of the ball-milled rutile {beta}-MnO{sub 2}vs cycles demonstrates a new system takes place from the second cycle, characterized by a significant improvement of Li diffusion by a factor 5 and a cathode impedance which decreases by a factor 2, remaining thereafter unchanged during cycling.

  10. Encapsulation of Prodan in beta-cyclodextrin environments: A critical study via electronic spectroscopy and molecular mechanics (United States)

    Banerjee, Anwesha; Sengupta, Bidisa; Chaudhuri, Sudip; Basu, Kaushik; Sengupta, Pradeep K.


    We present a detailed study on the binding of the naphthalene based fluorescence probe Prodan with two cyclic oligosachharides namely, natural beta-cyclodextrin (β-CD) and its synthetic derivative, succinyl-2-hydroxypropyl beta-cyclodextrin (SHPβ-CD) using electronic absorption and fluorescence spectroscopy along with theoretical techniques. The encapsulation of Prodan inside the β-CD cavities leads to pronounced changes in its emission characteristics, including dramatic blue shifts (27 nm in 10 mM SHPβ-CD and 19 nm in 10 mM β-CD) in the emission maximum accompanied by increase in the emission yield, fluorescence anisotropy and lifetime values. Detailed analyses of the fluorescence along with relevant absorption spectroscopic data indicate that Prodan readily enters the doughnut-shaped hydrophobic cavities of the β-CDs and forms 1:1 inclusion complexes, the binding affinity being significantly higher in case of SHPβ-CD. Furthermore, docking studies performed via molecular mechanics methods (MM+) indicate that the dimethylamino group of Prodan is most likely to be oriented towards the wider rim of the cyclodextrin cavity. Quantum mechanical calculations reveal that incorporation of Prodan into the β-CD cavities, results in the formation of a N-TICT (dimethylamino twisted intramolecular charge transfer) state.

  11. Gallium Arsenide detectors for X-ray and electron (beta particle) spectroscopy (United States)

    Lioliou, G.; Barnett, A. M.


    Results characterizing GaAs p+-i-n+ mesa photodiodes with a 10 μm i layer for their spectral response under illumination of X-rays and beta particles are presented. A total of 22 devices, having diameters of 200 μm and 400 μm, were electrically characterized at room temperature. All devices showed comparable characteristics with a measured leakage current ranging from 4 nA/cm2 to 67 nA/cm2 at an internal electric field of 50 kV/cm. Their unintentionally doped i layers were found to be almost fully depleted at 0 V due to their low doping density. 55Fe X-ray spectra were obtained using one 200 μm diameter device and one 400 μm diameter device. The best energy resolution (FWHM at 5.9 keV) achieved was 625 eV using the 200 μm and 740 eV using the 400 μm diameter device, respectively. Noise analysis showed that the limiting factor for the energy resolution of the system was the dielectric noise; if this noise was eliminated by better design of the front end of the readout electronics, the achievable resolution would be 250 eV. 63Ni beta particle spectra obtained using the 200 μm diameter device showed the potential utility of these detectors for electron and beta particle detection. The development of semiconductor electron spectrometers is important particularly for space plasma physics; such devices may find use in future space missions to study the plasma environment of Jupiter and Europa and the predicted electron impact excitation of water vapor plumes from Europa hypothesized as a result of recent Hubble Space Telescope (HST) UV observations.

  12. Exciton Coupling in Circular Dichroic Spectroscopy as a Tool for Establishing the Absolute Configuration of alpha,beta-Unsaturated Esters of Allylic Alcohols

    DEFF Research Database (Denmark)

    Lauridsen, A.; Cornett, Claus; Christensen, S. B.


    alpha-beta-Unsaturated esters of allylic alcohols have been shown to exhibit exciton coupling by circular dichroic spectroscopy. This coupling permits the establishment of the absolute configuration. The method was used to prove the absolute configuration at C-2 of archangelolide. Detailed NMR sp...

  13. Properties of the open cluster Tombaugh 1 from high resolution spectroscopy and uvbyCaH$\\beta$ photometry

    CERN Document Server

    Silva, João V Sales; Anthony-Twarog, Barbara J; Bidin, Christian Moni; Costa, Edgardo; Twarog, Bruce A


    Open clusters can be the key to deepen our knowledge on various issues involving the structure and evolution of the Galactic disk and details of stellar evolution because a cluster's properties are applicable to all its members. However the number of open clusters with detailed analysis from high resolution spectroscopy and/or precision photometry imposes severe limitation on studies of these objects. To expand the number of open clusters with well-defined chemical abundances and fundamental parameters, we investigate the poorly studied, anticenter open cluster Tombaugh 1. Using precision uvbyCaH$\\beta$ photometry and high resolution spectroscopy, we derive the cluster's properties and, for the first time, present detailed abundance analysis of 10 potential cluster stars. Using radial position from the cluster center and multiple color indices, we have isolated a sample of unevolved probable, single-star members of Tombaugh 1. The weighted photometric metallicity from $m_1$ and $hk$ is [Fe/H] = -0.10 $\\pm$ 0....

  14. Two-Dimensional Infrared (2DIR) Spectroscopy of the Peptide Beta3s Folding (United States)

    Lai, Zaizhi; Preketes, Nicholas K; Jiang, Jun; Mukamel, Shaul; Wang, Jin


    Probing underlying free energy landscape, pathways, and mechanism is the key for understanding protein folding in theory and experiment. Recently time-resolved two-dimensional infrared (2DIR) with femtosecond laser pulses, has emerged as a promising tool for investigating the protein folding dynamics on faster timescales than possible by NMR. We have employed molecular dynamics simulations to compute 2DIR spectra of the folding process of a peptide, Beta3s. Simulated non-chiral and chiral 2DIR signals illustrate the variation of the spectra as the peptide conformation evolves along the free energy landscape. Chiral spectra show stronger changes than the non-chiral signals because cross peaks caused by the formation of the β-sheet are clearly resolved. Chirality-induced 2DIR may be used to detect the folding of β-sheet proteins with high spectral and temporal resolution. PMID:23956818

  15. Shape-coexistence and shape-evolution studies for bismuth isotopes by insource laser spectroscopy and $\\beta$-delayed fission in $^{188}$Bi

    CERN Multimedia

    The proposal aims at the two main goals: \\\\ \\\\1) the studies of shape-coexistence and shape-evolution phenomena in the long chain of bismuth isotopes (Z=83) by in-source laser spectroscopy measurements of isotopic shifts (IS) and hyperfine structures (hfs), and \\\\ 2) $\\beta$-delayed fission ($\\beta$DF) of two isomeric states in $^{188}$Bi. \\\\ \\\\Isomer-selective $\\beta$DF studies for $^{188m1, 188m2}$Bi isomers will enable us for the first time to investigate the spin-dependence of the $\\beta$DF process and to check theoretical predictions of asymmetrical fission fragment mass-distribution in this region of nuclei. The measurements will be performed with the well-proven Windmill and MR-TOF MS/Penning Trap techniques.

  16. High-resolution metallic magnetic calorimeters for {beta}-spectroscopy on {sup 187}rhenium and position resolved X-ray spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Porst, Jan-Patrick


    This thesis describes the development of metallic magnetic calorimeters (MMCs) for high resolution spectroscopy. MMCs are energy dispersive particle detectors based on the calorimetric principle which are typically operated at temperatures below 100 mK. The detectors make use of a paramagnetic temperature sensor to transform the temperature rise upon the absorption of a particle in the detector into a measurable magnetic flux change in a dc-SQUID. The application of MMCs for neutrino mass measurements and their advantages with respect to other approaches are discussed. In view of this application the development of an MMC optimized for {beta}-endpoint spectroscopy on {sup 187}rhenium is presented. A fully micro-fabricated X-ray detector is characterized and performs close to design values. Furthermore, a new technique to more efficiently couple rhenium absorbers mechanically and thermally to the sensor was developed and successfully tested. By employing a metallic contact, signal rise times faster than 5 {mu}s could be observed with superconducting rhenium absorbers. In addition to the single pixel detectors, an alternative approach of reading out multiple pixels was developed in this work, too. Here, the individual absorbers have a different thermal coupling to only one temperature sensor resulting in a distribution of different pulse shapes. Straightforward position discrimination by means of rise time analysis is demonstrated for a four pixel MMC and a thermal model of the detector is provided. Unprecedented so far, an energy resolution of less than {delta}E{sub FWHM}<5 eV for 5.9 keV X-rays was achieved across all absorbers. (orig.)

  17. Cu K-edge X-ray Absorption Spectroscopy Reveals Differential Copper Coordimation Within Amyloid-beta Oligomers Compared to Amyloid-beta Monomers

    Energy Technology Data Exchange (ETDEWEB)

    J Shearer; P Callan; T Tran; V Szalai


    The fatal neurodegenerative disorder Alzheimer's disease (AD) has been linked to the formation of soluble neurotoxic oligomers of amyloid-{beta} (A{beta}) peptides. These peptides have high affinities for copper cations. Despite their potential importance in AD neurodegeneration few studies have focused on probing the Cu{sup 2+/1+} coordination environment within A{beta} oligomers. Herein we present a Cu K-edge X-ray absorption spectroscopic study probing the copper-coordination environment within oligomers of A{beta}(42) (sequence: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA). We find that the Cu{sup 2+} cation is contained within a square planar mixed N/O ligand environment within A{beta}(42) oligomers, which is similar to the copper coordination environment of the monomeric forms of {l_brace}Cu{sup II}A{beta}(40){r_brace} and {l_brace}Cu{sup II}A{beta}(16){r_brace}. Reduction of the Cu{sup 2+} cation within the A{beta}(42) oligomers to Cu{sup 1+} yields a highly dioxygen sensitive copper-species that contains Cu{sup 1+} in a tetrahedral coordination geometry. This can be contrasted with monomers of {l_brace}Cu{sup I}A{beta}(40){r_brace} and {l_brace}Cu{sup I}A{beta}(16){r_brace}, which contain copper in a dioxygen inert linear bis-histidine ligand environment [Shearer and Szalai, J. Am. Chem. Soc., 2008, 130, 17826]. The biological implications of these findings are discussed.

  18. Inclusion complexes of cypermethrin and permethrin with monochlorotriazinyl-beta-cyclodextrin: a combined spectroscopy, TG/DSC and DFT study. (United States)

    Yao, Qi; You, Bin; Zhou, Shuli; Chen, Meng; Wang, Yujiao; Li, Wei


    The suitable size hydrophobic cavity and monochlorotriazinyl group as a reactive anchor make MCT-β-CD to be widely used in fabric finishing. In this paper, the inclusion complexes of monochlorotriazinyl-beta-cyclodextrin (MCT-β-CD) with cypermethrin (CYPERM) and permethrin (PERM) are synthesized and analyzed by TG/DSC, FT-IR and Raman spectroscopy. TG/DSC reveals that the decomposed temperatures of inclusion complexes are lower by 25-30 °C than that of physical mixtures. DFT calculations in conjunction with FT-IR and Raman spectral analyses are used to study the structures of MCT-β-CD and their inclusion complexes. Four isomers of trisubstituted MCT-β-CD are designed and DFT calculations reveal that 1,3,5-trisubstituted MCT-β-CD has the lowest energy and can be considered as main component of MCT-β-CD. The ground-state geometries, vibrational wavenumbers, IR and Raman intensities of MCT-β-CD and their inclusion complexes were calculated at B3LYP/6-31G (d) level of theory. Upon examining the optimized geometry of inclusion complex, we find that the CYPERM and PERM are inserted into the toroid of MCT-β-CD from the larger opening. The band at 1646 cm(-1) in IR and at 1668 cm(-1) in Raman spectrum reveals that monochloroazinyl group of MCT-β-CD exists in ketone form but not in anion form. The noticeable IR and Raman shift of phenyl reveals that these two benzene rings of CYPERM and PERM stays inside the cavity of MCT-β-CD and has weak interaction with MCT-β-CD. This spectroscopy conclusion is consistent with theoretical predicted structure.

  19. Harmonic force spectroscopy reveals a force-velocity curve from a single human beta cardiac myosin motor (United States)

    Sung, Jongmin; Nag, Suman; Vestergaard, Christian; Mortensen, Kim; Flyvbjerg, Henrik; Spudich, James


    A muscle contracts rapidly under low load, but slowly under high load. Its molecular mechanisms remain to be elucidated, however. During contraction, myosins in thick filaments interact with actin in thin filaments in the sarcomere, cycling between a strongly bound (force producing) state and a weakly bound (relaxed) state. Huxley et al. have previously proposed that the transition from the strong to the weak interaction can be modulated by a load. We use a new method we call ``harmonic force spectroscopy'' to extract a load-velocity curve from a single human beta cardiac myosin II motor. With a dual-beam optical trap, we hold an actin dumbbell over a myosin molecule anchored to the microscope stage that oscillates sinusoidally. Upon binding, the motor experiences an oscillatory load with a mean that is directed forward or backward, depending on binding location We find that the bound time at saturating [ATP] is exponentially correlated with the mean load, which is explained by Arrhenius transition theory. With a stroke size measurement, we obtained a load-velocity curve from a single myosin. We compare the curves for wild-type motors with mutants that cause hypertrophic cardiomyopathies, to understand the effects on the contractile cycle

  20. Probing the Kinetic Anabolism of Poly-Beta-Hydroxybutyrate in Cupriavidus necator H16 Using Single-Cell Raman Spectroscopy

    Directory of Open Access Journals (Sweden)

    Zhanhua Tao


    Full Text Available Poly-beta-hydroxybutyrate (PHB can be formed in large amounts in Cupriavidus necator and is important for the industrial production of biodegradable plastics. In this investigation, laser tweezers Raman spectroscopy (LTRS was used to characterize dynamic changes in PHB content—as well as in the contents of other common biomolecule—in C. necator during batch growth at both the population and single-cell levels. PHB accumulation began in the early stages of bacterial growth, and the maximum PHB production rate occurred in the early and middle exponential phases. The active biosynthesis of DNA, RNA, and proteins occurred in the lag and early exponential phases, whereas the levels of these molecules decreased continuously during the remaining fermentation process until the minimum values were reached. The PHB content inside single cells was relatively homogenous in the middle stage of fermentation; during the late growth stage, the variation in PHB levels between cells increased. In addition, bacterial cells in various growth phases could be clearly discriminated when principle component analysis was performed on the spectral data. These results suggest that LTRS is a valuable single-cell analysis tool that can provide more comprehensive information about the physiological state of a growing microbial population.

  1. Spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Hellman, Hal


    This booklet discusses spectroscopy, the study of absorption of radiation by matter, including X-ray, gamma-ray, microwave, mass spectroscopy, as well as others. Spectroscopy has produced more fundamental information to the study of the detailed structure of matter than any other tools.

  2. Spectroscopy

    CERN Document Server

    Walker, S


    The three volumes of Spectroscopy constitute the one comprehensive text available on the principles, practice and applications of spectroscopy. By giving full accounts of those spectroscopic techniques only recently introduced into student courses - such as Mössbauer spectroscopy and photoelectron spectroscopy - in addition to those techniques long recognised as being essential in chemistry teaching - sucha as e.s.r. and infrared spectroscopy - the book caters for the complete requirements of undergraduate students and at the same time provides a sound introduction to special topics for graduate students.

  3. $\\beta$3$p$-spectroscopy and proton-$\\gamma$ width determination in the decay of $^{31}$Ar

    CERN Multimedia

    We propose to perform a detailed study of the $\\beta$-decay of the dripline nucleus $^{31}$Ar. This will allow a detailed study of the $\\beta$-delayed 3$p$-decay as well as provide important information on the resonances of $^{30}$S and $^{29}$P, in particular the ratio between the $p$- and $\\gamma$- partial widths relevant for astrophysics.

  4. Study of inclusion complex formation between tropaeolin OO and beta-cyclodextrin by spectrophotometry and Infrared spectroscopy. (United States)

    Wang, Huai You; Han, Juan; Feng, Xia Guang; Pang, Yan Ling


    The mechanism of the inclusion of tropaeolin OO (TPOO) and beta-cyclodextrin (beta-CD) has been studied by spectrophotometry. The inclusion depth of the guest molecule in the host molecule was demonstrated by infrared spectrometry. Effect of the pH, concentrations of beta-CD, solvents and ionic strength on the inclusion of TPOO and beta-CD were examined. The result showed that TPOO reacts with beta-CD to form a 1:1 host-guest complex with an apparent formation constant of 1.50 x 10(3) l mol(-1). The thermodynamic parameters of inclusion reaction, DeltaG degrees , DeltaH degrees and DeltaS degrees were obtained.

  5. Primary structure determination of five sialylated oligosaccharides derived from bronchial mucus glycoproteins of patients suffering from cystic fibrosis. The occurrence of the NeuAc alpha(2----3)Gal beta(1----4)[Fuc alpha(1----3)] GlcNAc beta(1----.) structural element revealed by 500-MHz 1H NMR spectroscopy. (United States)

    Lamblin, G; Boersma, A; Klein, A; Roussel, P; van Halbeek, H; Vliegenthart, J F


    The structure of sialylated carbohydrate units of bronchial mucins obtained from cystic fibrosis patients was investigated by 500-MHz 1H NMR spectroscopy in conjunction with sugar analysis. After subjecting the mucins to alkaline borohydride degradation, sialylated oligosaccharide-alditols were isolated by anion-exchange chromatography and fractionated by high performance liquid chromatography. Five compounds could be obtained in a rather pure state; their structures were established as the following: A-1, NeuAc alpha(2----3)Gal beta(1----4) [Fuc alpha(1----3)]GlcNAc beta(1----3)Gal-NAc-ol; A-2, NeuAc alpha(2----3)Gal beta(1----4)GlcNAc beta(1----6)-[GlcNAc beta (1----3)]GalNAc-o1; A-3, NeuAc alpha(2----3)Gal beta-(1----4)[Fuc alpha(1----3)]GlcNAc beta(1----3)Gal beta(1----3) GalNAc-o1; A-4, NeuAc alpha(2----3)Gal beta(1----4)[Fuc alpha(1----3)]Glc-NAc NAc beta(1----6)[GlcNAc beta(1----3)]GalNAc-o1; A-6,NeuAc alpha-(2----3) Gal beta(1----4)[Fuc alpha(1----3)]GlcNAc beta(1----6)[Gal beta-(1----4) GlcNAc beta(1----3)]GalNAc-o1. The simultaneous presence of sialic acid in alpha(2----3)-linkage to Gal and fucose in alpha(1----3)-linkage to GlcNAc of the same N-acetyllactosamine unit could be adequately proved by high resolution 1H NMR spectroscopy. This sequence constitutes a novel structural element for mucins.

  6. Spectroscopy

    DEFF Research Database (Denmark)

    Berg, Rolf W.

    This introductory booklet covers the basics of molecular spectroscopy, infrared and Raman methods, instrumental considerations, symmetry analysis of molecules, group theory and selection rules, as well as assignments of fundamental vibrational modes in molecules.......This introductory booklet covers the basics of molecular spectroscopy, infrared and Raman methods, instrumental considerations, symmetry analysis of molecules, group theory and selection rules, as well as assignments of fundamental vibrational modes in molecules....

  7. Hith resolution {beta}-spectroscopy of the isotope {sup 36}Cl using magnetic calorimeters; Entwicklung magnetischer Mikrokalorimeter fuer die hochaufloesende Spektroskopie des {beta}-Emitters {sup 36}Cl

    Energy Technology Data Exchange (ETDEWEB)

    Rotzinger, H.


    This thesis describes the development of a high resolution magnetic calorimeter for the detection of the {beta}-spectrum of the isotope {sup 36}Cl with endpoint energy of 709.6 keV. The temperature rise of a metallic paramagnetic sensor due to an energy deposition is sensed by measuring its magnetization using a sensitive DC-SQUID magnetometer. For a high detection efficiency an 4{pi} gold absorber was used. The heat capacity and the geometry of the absorber is optimally matched by a flat sensor and an optimized meander shaped readout coil. The fabrication of the superconducting structures and the detector setup are described. In addition, the relevant noise sources, the energy resolution and the quantum efficiency are discussed. A measured {sup 36}Cl-spectrum with an energy resolution of {delta}E{sub FWHM}=750 eV is presented and compared with existing experimental and theoretical data. (orig.)

  8. Cross-strand coupling of a beta-hairpin peptide stabilized with an Aib-Gly turn studied using isotope-edited IR spectroscopy. (United States)

    Huang, Rong; Setnicka, Vladimir; Etienne, Marcus A; Kim, Joohyun; Kubelka, Jan; Hammer, Robert P; Keiderling, Timothy A


    Isotope-edited IR spectroscopy was used to study a series of singly and doubly 13C=O-labeled beta-hairpin peptides stabilized by an Aib-Gly turn sequence. The double-labeled peptides have amide I' IR spectra that show different degrees of vibrational coupling between the 13C-labeled amides due to variations in the local geometry of the peptide structure. The single-labeled peptides provide controls to determine frequencies characteristic of the diagonal force field (FF) contributions at each position for the uncoupled 13C=O modes. Separation of diagonal FF and coupling effects on the spectra are used to explain the cross-strand labeled spectral patterns. DFT calculations based on an idealized model beta-hairpin peptide correctly predict the vibrational coupling patterns. Extending these model results by consideration of frayed ends and the hairpin conformational flexibility yields an alternate interpretation of details of the spectra. Temperature-dependent isotopically labeled IR spectra reveal differences in the thermal stabilities of the individual isotopically labeled sites. This is the first example of using an IR-based isotopic labeling technique to differentiate structural transitions at specific sites along the peptide backbone in model beta-hairpin peptides.

  9. Characterization of the alpha and beta subunits of casein kinase 2 by far-UV CD spectroscopy

    DEFF Research Database (Denmark)

    Issinger, O G; Brockel, C; Boldyreff, B;


    Although Chou-Fasman calculations of the secondary structure of recombinant casein kinase 2 subunits alpha and beta suggest they have a similar overall conformation, circular dichroism (CD) studies show that substantial differences in the conformation of the two subunits exist. In addition......, no changes in the far-UV CD spectrum of the alpha subunit are observed in the presence of casein or the synthetic decapeptide substrate RRRDDDSDDD. Furthermore, the alpha-helical structure of the alpha subunit (but not the beta subunit) can be increased in the presence of stoichiometric amounts of heparin...

  10. Orbital parameters, masses and distance to beta Centauri determined with the Sydney University Stellar Interferometer and high-resolution spectroscopy

    NARCIS (Netherlands)

    Davis, J.; Mendez, A.; Seneta, E.B.; Tango, W.J.; Booth, A.J.; O'Byrne, J.W.; Thorvaldson, E.D.; Ausseloos, M.; Aerts, C.C.; Uytterhoeven, K.


    The bright southern binary star beta Centauri (HR5267) has been observed with the Sydney University Stellar Interferometer (SUSI) and spectroscopically with the European Southern Observatory Coude Auxiliary Telescope and Swiss Euler telescope at La Silla. The interferometric observations have confir

  11. $\\beta$-delayed neutron spectroscopy of $^{130-132}$ Cd isotopes with the ISOLDE decay station and the VANDLE array

    CERN Multimedia

    We propose to use the new ISOLDE decay station and the neutron detector VANDLE to measure the $\\beta$-delayed neutron emission of N=82-84 $^{130-132}$Cd isotopes. The large delayed neutron emission probability observed in a previous ISOLDE measurement is indicative of the Gamow-Teller transitions due to the decay of deep core neutrons. Core Gamow-Teller decay has been experimentally proven in the $^{78}$Ni region for the N>50 nuclei using the VANDLE array. The spectroscopic measurement of delayed neutron emission along the cadmium isotopic chain will allow us to track the evolution of the single particle states and the shell gap.

  12. A NIM (Nuclear Instrumentation Module) system conjugated with optional input for pHEMT amplifier for beta and gamma spectroscopy; Um sistema de modulos NIM conjugados com entrada opcional por amplificador pHEMT para espectroscopia beta e gama

    Energy Technology Data Exchange (ETDEWEB)

    Konrad, Barbara; Lüdke, Everton, E-mail:, E-mail: [Universidade Federal de Santa Maria (LAE/UFSM), RS (Brazil). Lab. de Astrofisica e Eletronica


    This work presents a high speed NIM module (Nuclear Instrumentation Module) to detect radiation, gamma and muons, as part of a system for natural radiation monitoring and of extraterrestrial origin. The subsystem developed consists of a preamplifier and an integrated SCA (Single Channel Analyzer), including power supplies of ± 12 and ± 24V with derivations of +3.6 and ± 5V. The single channel analyzer board, consisting of discrete logic components, operating in window modes, normal and integral. The pulse shaping block is made up of two voltage comparators working at 120 MHz with a response time > 60 ns and a logic anticoincidence system. The preamplifier promotes a noise reduction and introduces the impedance matching between the output of anode / diode photomultiplier tubes (PMTs) and subsequent equipment, providing an input impedance of 1MΩ and output impedance of 40 to 140Ω. The shaper amplifier is non-inverting and has variable input capacitance of 1000 pF. The upper and lower thresholds of the SCA are adjustable from 0 to ± 10V, and the equipment is compatible with various types of detectors, like PMTs coupled to sodium iodide crystals. For use with liquid scintillators and photodiodes with crystals (CsI: Tl) is proposed to include a preamplifier circuit pHEMT (pseudomorphic High Electron Mobility Transistor) integrated. Yet, the system presents the possibility of applications for various purposes of gamma spectroscopy and automatic detection of events producing of beta particles.

  13. Orbital parameters, masses and distance to Beta Centauri determined with the Sydney University Stellar Interferometer and high resolution spectroscopy

    CERN Document Server

    Davis, J; Seneta, E B; Tango, W J; Booth, A J; O'Byrne, J W; Thorvaldson, E D; Ausseloos, M; Aerts, C; Uytterhoeven, K


    The bright southern binary star beta Centauri (HR 5267) has been observed with the Sydney University Stellar Interferometer (SUSI) and spectroscopically with the ESO CAT and Swiss Euler telescopes at La Silla. The interferometric observations have confirmed the binary nature of the primary component and have enabled the determination of the orbital parameters of the system. At the observing wavelength of 442 nm the two components of the binary system have a magnitude difference of 0.15. The combination of interferometric and spectroscopic data gives the following results: orbital period 357 days, semi-major axis 25.30 mas, inclination 67.4 degrees, eccentricity 0.821, distance 102.3 pc, primary and secondary masses M1 = M2 = 9.1 solar masses and absolute visual magnitudes of the primary and secondary M1V = -3.85 and M2V = -3.70. The high accuracy of the results offers a fruitful starting point for future asteroseismic modelling of the pulsating binary components.

  14. Conformational analysis of 3-(trimethylsilyl)propionic acid by NMR spectroscopy: an unusual expression of the beta-silyl effect. (United States)

    Nkansah, Richard A; Gerken, James B; Roberts, John D


    The rotational freedom of the carbon-carbon single bonds of 1,2-disubstituted ethanes affords the possibility of these compounds existing as a rapidly interconverting mixture of conformers in solution. The conformational preferences of one such compound, 3-(trimethylsilyl)propionic acid, and its anion were studied in water, dimethyl sulfoxide, methanol, ethanol, isopropyl alcohol, tert-butyl alcohol, tetrahydrofuran, and toluene with 1H NMR spectroscopy. The conformational preferences were determined from the vicinal proton-proton coupling constants between the hydrogen nuclei of the CH(2)CH(2) group with the aid of the Altona equations to derive the equilibrium anti and gauche percentages of rotamers from the averaged NMR-time scale couplings. Conformational analyses of 4,4-dimethylpentanoic acid and its anion as well as 2-(trimethylsilyl)ethanesulfonate anion were also conducted to compare the relative structural influences on the conformational preferences of silicon and carbon.

  15. Ground state properties of neutron-rich Mg isotopes the "island of inversion" studied with laser and $\\beta$-NMR spectroscopy

    CERN Document Server

    Kowalska, M


    Studies in regions of the nuclear chart in which the model predictions of properties of nuclei fail can bring a better understanding of the strong interaction in the nuclear medium. To such regions belongs the so called "island of inversion" centered around Ne, Na and Mg isotopes with 20 neutrons in which unexpected ground-state spins, large deformations and dense low-energy spectra appear. This is a strong argument that the magic N=20 is not a closed shell in this area. In this thesis investigations of isotope shifts of stable $^{24-26}$Mg, as well as spins and magnetic moments of short-lived $^{29,31}$Mg are presented. The successful studies were performed at the ISOLDE facility at CERN using collinear laser and $\\beta$-NMR spectroscopy techniques. The isotopes were investigated as single-charged ions in the 280 nm transition from the atomic ground state $^2\\!$S$_{1/2}$ to one of the two lowest excited states $^2\\!$P$_{1/2 ,\\,3/2}$ using continuous wave laser beams. The isotope-shift measurements with fluor...

  16. Hydrogenation of the alpha,beta-Unsaturated Aldehydes Acrolein, Crotonaldehyde, and Prenal over Pt Single Crystals: A Kinetic and Sum-Frequency Generation Vibrational Spectroscopy Study

    Energy Technology Data Exchange (ETDEWEB)

    Kliewer, C.J.; Somorjai, G.A.


    Sum-frequency generation vibrational spectroscopy (SFG-VS) and kinetic measurements using gas chromatography have been used to study the surface reaction intermediates during the hydrogenation of three {alpha},{beta}-unsaturated aldehydes, acrolein, crotonaldehyde, and prenal, over Pt(111) at Torr pressures (1 Torr aldehyde, 100 Torr hydrogen) in the temperature range of 295K to 415K. SFG-VS data showed that acrolein has mixed adsorption species of {eta}{sub 2}-di-{sigma}(CC)-trans, {eta}{sub 2}-di-{sigma}(CC)-cis as well as highly coordinated {eta}{sub 3} or {eta}{sub 4} species. Crotonaldehyde adsorbed to Pt(111) as {eta}{sub 2} surface intermediates. SFG-VS during prenal hydrogenation also suggested the presence of the {eta}{sub 2} adsorption species, and became more highly coordinated as the temperature was raised to 415K, in agreement with its enhanced C=O hydrogenation. The effect of catalyst surface structure was clarified by carrying out the hydrogenation of crotonaldehyde over both Pt(111) and Pt(100) single crystals while acquiring the SFG-VS spectra in situ. Both the kinetics and SFG-VS showed little structure sensitivity. Pt(100) generated more decarbonylation 'cracking' product while Pt(111) had a higher selectivity for the formation of the desired unsaturated alcohol, crotylalcohol.

  17. Hydrogenation of the alpha,beta-unsaturated aldehydes acrolein, crotonaldehyde, and prenal over Pt single crystals: a kinetic and sum-frequency generation vibrational spectroscopy study. (United States)

    Kliewer, Christopher J; Bieri, Marco; Somorjai, Gabor A


    Sum-frequency generation vibrational spectroscopy (SFG-VS) and kinetic measurements using gas chromatography have been used to study the surface reaction intermediates during the hydrogenation of three alpha,beta-unsaturated aldehydes, acrolein, crotonaldehyde, and prenal, over Pt(111) at Torr pressures (1 Torr of aldehyde, 100 Torr of hydrogen) in the temperature range of 295-415 K. SFG-VS data showed that acrolein has mixed adsorption species of eta(2)-di-sigma(CC)-trans, eta(2)-di-sigma(CC)-cis as well as highly coordinated eta(3) or eta(4) species. Crotonaldehyde adsorbed to Pt(111) as eta(2) surface intermediates. SFG-VS during prenal hydrogenation also suggested the presence of the eta(2) adsorption species and became more highly coordinated as the temperature was raised to 415 K, in agreement with its enhanced C=O hydrogenation. The effect of catalyst surface structure was clarified by carrying out the hydrogenation of crotonaldehyde over both Pt(111) and Pt(100) single crystals while acquiring the SFG-VS spectra in situ. Both the kinetics and SFG-VS showed little structure sensitivity. Pt(100) generated more decarbonylation "cracking" product while Pt(111) had a higher selectivity for the formation of the desired unsaturated alcohol, crotyl alcohol.

  18. Growth, shrinking, and breaking of pluronic micelles in the presence of drugs and/or beta-cyclodextrin, a study by small-angle neutron scattering and fluorescence spectroscopy. (United States)

    Valero, Margarita; Dreiss, Cécile A


    The associative structures between F127 Pluronic micelles and four drugs, namely, lidocaine (LD), pentobarbital sodium salt (PB), sodium naproxen (NP), and sodium salicylate (SAL), were studied by small-angle neutron scattering (SANS). Different outcomes for the micellar aggregates are observed, which are dependent on the chemical nature of the drug and the presence of charge or otherwise: the micelles grow with LD, are hardly modified with PB, and decrease in size with both NP and SAL. The partition coefficient, determined by fluorescence spectroscopy, is directly correlated to the amount of charge, following NP approximately SAL inclusion complexes with heptakis(2,6-di-O-methyl) beta-cyclodextrin (hep2,6 beta-CD). Hep2,6 beta-CD, as shown in previous studies (Joseph, J.; Dreiss, C. A.; Cosgrove, T. Langmuir, 2008, 24, 10005-10010; Dreiss, C. A.; Nwabunwanne, E.; Liu, R.; Brooks, N. J. Soft Matter, 2009, 5, 1888-1896), is also able to form a complex with F127, resulting in micellar breakup. In the ternary mixtures, a fine balance of forces is involved, which results in drastic micellar changes, as observed from the SANS patterns. Depending on the ratio of drug, polymer, and hep2,6 beta-CD and the nature of the interactions (which is directly linked to the drug chemical structure), the presence of drug either hinders micellar breakup by beta-CD (at high enough concentration of LD or PB) or leads to micellar growth (NP). These effects are mainly attributed to a preferential drug/beta-CD interaction (except for PB), which, at least in the conditions studied here, explains the higher beta-CD concentration needed for micellar breakup to occur.

  19. Beta Thalassemia (United States)

    Beta thalassemia is found in people of Mediterranean, Middle Eastern, African, South Asian (Indian, Pakistani, etc.), Southeast Asian and Chinese descent. 1 Beta Thalassemia ßß Normal beta globin genes found on chromosomes ...

  20. Reduced rate of adenosine triphosphate synthesis by in vivo 31P nuclear magnetic resonance spectroscopy and downregulation of PGC-1beta in distal skeletal muscle following burn. (United States)

    Tzika, A Aria; Mintzopoulos, Dionyssios; Padfield, Katie; Wilhelmy, Julie; Mindrinos, Michael N; Yu, Hongue; Cao, Haihui; Zhang, Qunhao; Astrakas, Loukas G; Zhang, Jiangwen; Yu, Yong-Ming; Rahme, Laurence G; Tompkins, Ronald G


    Using a mouse model of burn trauma, we tested the hypothesis that severe burn trauma corresponding to 30% of total body surface area (TBSA) causes reduction in adenosine triphosphate (ATP) synthesis in distal skeletal muscle. We employed in vivo 31P nuclear magnetic resonance (NMR) in intact mice to assess the rate of ATP synthesis, and characterized the concomitant gene expression patterns in skeletal muscle in burned (30% TBSA) versus control mice. Our NMR results showed a significantly reduced rate of ATP synthesis and were complemented by genomic results showing downregulation of the ATP synthase mitochondrial F1 F0 complex and PGC-1beta gene expression. Our findings suggest that inflammation and muscle atrophy in burns are due to a reduced ATP synthesis rate that may be regulated upstream by PGC-1beta. These findings implicate mitochondrial dysfunction in distal skeletal muscle following burn injury. That PGC-1beta is a highly inducible factor in most tissues and responds to common calcium and cyclic adenosine monophosphate (cAMP) signaling pathways strongly suggests that it may be possible to develop drugs that can induce PGC-1beta.

  1. Evidence for Intramolecular Antiparallel Beta-Sheet Structure in Alpha-Synuclein Fibrils from a Combination of Two-Dimensional Infrared Spectroscopy and Atomic Force Microscopy

    NARCIS (Netherlands)

    Roeters, Steven J.; Iyer, Aditya; Pletikapic, Galja; Kogan, Vladimir; Subramaniam, Vinod; Woutersen, Sander


    The aggregation of the intrinsically disordered protein alpha-synuclein (αS) into amyloid fibrils is thought to play a central role in the pathology of Parkinson’s disease. Using a combination of techniques (AFM, UV-CD, XRD, and amide-I 1D- and 2D-IR spectroscopy) we show that the structure of αS fi

  2. Shape effects along the Z=82 line: study of the $\\beta$- decay of $^{188,190,192}$Pb using total absorption spectroscopy

    CERN Multimedia

    Caballero ontanaya, L; Garcia borge, M J; Malbrunot, S


    This proposal is aimed at the study of the $\\beta$- decay of the neutron-deficient $^{188,190,192}$Pb nuclei. The main motivation of the proposed experiment is to determine the Gamow-Teller strength distribution in the daughter nuclei using the Total Absorption Spectrometer "Lucrecia". Recent theoretical results show that from this measurement the shapes of the ground states of the decaying Pb nuclei can be inferred. This study offers an independent way to study the phenomenon of shape co-existence in a region of particular interest.

  3. Ultrafast relaxation kinetics of the dark S{sub 1} state in all-trans-{beta}-carotene explored by one- and two-photon pump-probe spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Kosumi, Daisuke, E-mail: [Department of Physics, Graduate School of Science, Tohoku University, 6-3 Aramaki-Aza-Aoba, Sendai 980-8578 (Japan); Abe, Kenta; Karasawa, Hiroshi [Department of Physics, Graduate School of Science, Tohoku University, 6-3 Aramaki-Aza-Aoba, Sendai 980-8578 (Japan); Fujiwara, Masazumi [Department of Physics, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585 (Japan); Cogdell, Richard J. [Glasgow Biomedical Research Center, University of Glasgow, 120 University Place, Glasgow G12 8TA, Scotland (United Kingdom); Hashimoto, Hideki [Department of Physics, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585 (Japan); JST/CREST, 4-1-8 Hon-chou, Kawaguchi, Saitama 332-0012 (Japan); Yoshizawa, Masayuki [Department of Physics, Graduate School of Science, Tohoku University, 6-3 Aramaki-Aza-Aoba, Sendai 980-8578 (Japan); JST/CREST, 4-1-8 Hon-chou, Kawaguchi, Saitama 332-0012 (Japan)


    Femtosecond one- and two-photon pump-probe dispersive spectroscopic measurements have been applied to the investigation of the vibrational relaxation kinetics of the dark S{sub 1} (2{sup 1}A{sub g}{sup -}) state in {beta}-carotene, combining a higher sensitive detection system with tunable visible and infrared excitation pulses. The two-photon excitation measurements enable the preferential detection of the dark S{sub 1} state. The tunable infrared excitation pulses allowed selective excitation to a different vibrational level of S{sub 1}. The S{sub 1} dynamics at early delay times depend strongly on excitation energy. A dependence of the initial S{sub 1} dynamics on excitation energy is discussed in term of the vibrational relaxation of S{sub 1}.

  4. Ultrafast transient lens spectroscopy of various C40 carotenoids: lycopene, beta-carotene, (3R,3'R)-zeaxanthin, (3R,3'R,6'R)-lutein, echinenone, canthaxanthin, and astaxanthin. (United States)

    Kopczynski, Matthäus; Lenzer, Thomas; Oum, Kawon; Seehusen, Jaane; Seidel, Marco T; Ushakov, Vladimir G


    The ultrafast internal conversion (IC) dynamics of seven C(40) carotenoids have been investigated at room temperature in a variety of solvents using two-color transient lens (TL) pump-probe spectroscopy. We provide comprehensive data sets for the carbonyl carotenoids canthaxanthin, astaxanthin, and-for the first time-echinenone, as well as new data for lycopene, beta-carotene, (3R,3'R)-zeaxanthin and (3R,3'R,6'R)-lutein in solvents which have not yet been investigated in the literature. Measurements were carried out to determine, how the IC processes are influenced by the conjugation length of the carotenoids, additional substituents and the polarity of the solvent. TL signals were recorded at 800 nm following excitation into the high energy edge of the carotenoid S2 band at 400 nm. For the S2 lifetime solvent-independent upper limits on the order of 100-200 fs are estimated for all carotenoids studied. The S1 lifetimes are in the picosecond range and increase systematically with decreasing conjugation length. For instance, in the sequence canthaxanthin/echinenone/beta-carotene (13/12/11 double bonds) one finds tau1 approximately 5, 7.7 and 9 ps for the S1-->S0 IC process, respectively. Hydroxyl groups not attached to the conjugated system have no apparent influence on tau1, as observed for canthaxanthin/astaxanthin (tau1 approximately 5 ps in both cases). For all carotenoids studied, tau1 is found to be insensitive to the solvent polarity. This is particularly interesting in the case of echinenone, canthaxanthin and astaxanthin, because earlier measurements for other carbonyl carotenoids like, e.g., peridinin partly showed dramatic differences. The likely presence of an intramolecular charge transfer state in the excited state manifold of C40 carbonyl carotenoids, which is stabilized in polar solvents, has obviously no influence on the measured tau1.

  5. Analysis of the interactions of mixtures of two beta-agonists steroids with bovine serum albumin: a fluorescence spectroscopy and chemometrics investigation. (United States)

    Ni, Yongnian; Zhang, Qiulan; Kokot, Serge


    Beta-agonists such as ractopamine (RAC) and clenbuterol (CLEN), have similar effects as anabolic steroids i.e. they promote growth of muscular tissue and reduce body fat. They have been used successfully with animals and humans but have also been banned in many countries principally, because of their serious side effects. However, their illegal use persists. Thus, their interaction with biomolecules such as bovine serum albumin (BSA) is of significance, especially the co-operative reaction of mixed ligands with the protein. Fluorescence and UV-vis spectra of complex mixtures of individual ligands, binary and ternary complexes with BSA resulted in significantly overlapping spectral profiles. Qualitative and quantitative information about the various complex ligand-protein species formed, was obtained with the resolution of the excitation-emission fluorescence three-way data matrices by chemometrics methods-MCR-ALS and PARAFAC. Individual spectra of the ligands, their binary complexes with BSA and their ternary complexes were extracted, and quantitative concentration profiles for each species in a particular interaction were constructed. Such analyses made it possible to interpret the role and behaviour of each reaction component. It was found that both ligands, RAC and CLEN, bound co-operatively in site I of the BSA. This was confirmed with the use of site markers such as warfarin (site I) and ibuprofen (site II). However, CLEN formed a 1:1 CLEN-BSA complex, while RAC formed a 2:1 RAC(2)-BSA binary species. Interestingly, when CLEN or RAC was added to RAC(2)-BSA or CLEN-BSA, respectively, ternary complexes were produced such as RAC(2)-BSA-CLEN. Significantly, the presence of the second ligand in such an interaction in excess, appeared to increase the affinity of the added ligand for BSA. This may have consequences on the amount of steroid required to achieve a desired tissue growth effect.

  6. Study of the N=28 shell closure by one neutron transfer reaction: astrophysical application and {beta}-{gamma} spectroscopy of neutron rich nuclei around N=32/34 and N=40; Etude de la fermeture de couche N=28 autour du noyau {sub 18}{sup 46}Ar{sub 28} par reaction de transfert d'un neutron: application a l'astrophysique et Spectroscopie {beta}-{gamma} de noyaux riches en neutrons de N=32/34 et N=40

    Energy Technology Data Exchange (ETDEWEB)

    Gaudefroy, L


    The study of the N=28 shell closure has been presented as well as its astrophysical implications. Moreover the structure of neutron rich nuclei around N=32/34 and 40 was studied. The N=28 shell closure has been studied trough the one neutron transfer reaction on {sup 44,46}Ar nuclei. Excitation energies of states in {sup 45,47}Ar nuclei have been obtained, as well as their angular momenta and spectroscopic factors. These results were used to show that N=28 is still a good magic number in the argon isotopic chain. We interpreted the evolution of the spin-orbit partner gaps in terms of the tensor monopolar proton-neutron interaction. Thanks to this latter, we showed it is not necessary to summon up a reduction of the intensity of the spin-orbit force in order to explain this evolution in N=29 isotopes from calcium to argon chains. The neutron capture rates on {sup 44,46}Ar have been determined thanks to the results of the transfer reaction. Their influence on the nucleosynthesis of {sup 46,48}Ca was studied. We proposed stellar conditions to account for the abnormal isotopic ratio observed in the Allende meteorite concerning {sup 46,48}Ca isotopes. The beta decay and gamma spectroscopy of neutron rich nuclei in the scandium to cobalt region has been studied. We showed that beta decay process is dominated by the {nu}f{sub 5/2} {yields} {pi}f{sub 7/2} Gamow-Teller transition. Moreover, we demonstrated that the {nu}g{sub 9/2} hinders this process in the studied nuclei, and influences their structure, by implying the existence of isomers. Our results show that N=34 is not a magic number in the titanium chain and the superior ones. (author)

  7. Polymorphic differences in alpha- and beta-form crystals of 2R, 4S, 6-fluoro-2-methyl-spiro[chroman-4,4'-imidazoline]-2',5-dione (M79175) as determined by X-ray diffraction, infrared spectroscopy, and differential scanning calorimetry. (United States)

    Ashizawa, K; Uchikawa, K; Hattori, T; Sato, T; Miyake, Y


    Polymorphic differences in alpha- and beta-form crystals of 2R, 4S, 6-fluoro-2-methyl-spiro[chroman-4,4'-imidazoline]-2',5-dione (M79175; 1) were studied by X-ray diffractometry, infrared spectroscopy, and differential scanning calorimetry. X-ray powder diffraction indicated the longest spacing of the unit cells to be 14.9 and 12.4 A for the alpha- and beta-form crystals, respectively. The infrared spectra showed the absorption band assigned to NH streching vibration for the alpha-form crystals to be centered at 3250 cm-1 and that for the beta-form crystals to split into two peaks, at 3150 and 3425 cm-1. The enthalpies of fusion were 26.3 kJ/mol at 517.5 K and 31.3 kJ/mol at 501.0 K, respectively. Transformation from the beta- to alpha-form was observed at various heating rates, which were enhanced by the presence of a small amount of alpha-form crystals previously added to the beta-form. The former appeared to serve as a source of nuclei for the growth of both forms. These results confirm that the alpha-form crystal is more stable than the beta-form.

  8. In vivo and ex vivo 19-fluorine magnetic resonance imaging and spectroscopy of beta-cells and pancreatic islets using GLUT-2 specific contrast agents. (United States)

    Liang, Sayuan; Louchami, Karim; Kolster, Hauke; Jacobsen, Anna; Zhang, Ying; Thimm, Julian; Sener, Abdullah; Thiem, Joachim; Malaisse, Willy; Dresselaers, Tom; Himmelreich, Uwe


    The assessment of the β-cell mass in experimental models of diabetes and ultimately in patients is a hallmark to understand the relationship between reduced β-cell mass/function and the onset of diabetes. It has been shown before that the GLUT-2 transporter is highly expressed in both β-cells and hepatocytes and that D-mannoheptulose (DMH) has high uptake specificity for the GLUT-2 transporter. As 19-fluorine MRI has emerged as a new alternative method for MRI cell tracking because it provides potential non-invasive localization and quantification of labeled cells, the purpose of this project is to validate β-cell and pancreatic islet imaging by using fluorinated, GLUT-2 targeting mannoheptulose derivatives ((19) FMH) both in vivo and ex vivo. In this study, we confirmed that, similar to DMH, (19) FMHs inhibit insulin secretion and increase the blood glucose level in mice temporarily (approximately two hours). We were able to assess the distribution of (19) FMHs in vivo with a temporal resolution of about 20 minutes, which showed a quick removal of (19) FMH from the circulation (within two hours). Ex vivo MR spectroscopy confirmed a preferential uptake of (19) FMH in tissue with high expression of the GLUT-2 transporter, such as liver, endocrine pancreas and kidney. No indication of further metabolism was found. In summary, (19) FMHs are potentially suitable for visualizing and tracking of GLUT-2 expressed cells. However, current bottlenecks of this technique related to the quick clearance of the compound and relative low sensitivity of (19) F MRI need to be overcome. Copyright © 2016 John Wiley & Sons, Ltd.

  9. Evidence for Intramolecular Antiparallel Beta-Sheet Structure in Alpha-Synuclein Fibrils from a Combination of Two-Dimensional Infrared Spectroscopy and Atomic Force Microscopy (United States)

    Roeters, Steven J.; Iyer, Aditya; Pletikapić, Galja; Kogan, Vladimir; Subramaniam, Vinod; Woutersen, Sander


    The aggregation of the intrinsically disordered protein alpha-synuclein (αS) into amyloid fibrils is thought to play a central role in the pathology of Parkinson’s disease. Using a combination of techniques (AFM, UV-CD, XRD, and amide-I 1D- and 2D-IR spectroscopy) we show that the structure of αS fibrils varies as a function of ionic strength: fibrils aggregated in low ionic-strength buffers ([NaCl] ≤ 25 mM) have a significantly different structure than fibrils grown in higher ionic-strength buffers. The observations for fibrils aggregated in low-salt buffers are consistent with an extended conformation of αS molecules, forming hydrogen-bonded intermolecular β-sheets that are loosely packed in a parallel fashion. For fibrils aggregated in high-salt buffers (including those prepared in buffers with a physiological salt concentration) the measurements are consistent with αS molecules in a more tightly-packed, antiparallel intramolecular conformation, and suggest a structure characterized by two twisting stacks of approximately five hydrogen-bonded intermolecular β-sheets each. We find evidence that the high-frequency peak in the amide-I spectrum of αS fibrils involves a normal mode that differs fundamentally from the canonical high-frequency antiparallel β-sheet mode. The high sensitivity of the fibril structure to the ionic strength might form the basis of differences in αS-related pathologies.

  10. Radiation-induced polymerization of {beta}(+)-pinene and synthesis of optically active {beta}(+)/{beta}(-)pinene polymers and copolymers

    Energy Technology Data Exchange (ETDEWEB)

    Cataldo, Franco, E-mail: franco.cataldo@fastwebnet.i [Lupi Chemical Research, Via Casilina 1626/A, 00133 Rome (Italy); Lilla, Edo; Ursini, Ornella [Institute of Chemical Methodologies, CNR, Via Salaria Km. 29300, Monterotondo Stazione 00016, Rome (Italy)


    Poly-{beta}(+)-pinene (pB(+)p) was synthesized with {gamma} irradiation of the monomer {beta}(+)-pinene in bulk under vacuum at 1181 kGy. Also scalemic mixtures of {beta}(+)-pinene and {beta}(-)-pinene were irradiated at 1181 kGy to obtain synthetic copolymers of pB(+)/B(-)p. For comparison also {beta}(-)-pinene was converted into poly-{beta}(-)-pinene (pB(-)p) under the identical conditions adopted for its enantiomer. Furthermore pB(+)p and pB(-)p were also synthesized by thermal processing under the action of a chemical free radical initiator. The optical rotatory dispersion (ORD) of all pBp resins synthesized were accurately studied in the spectral range comprised between 375 and 625 nm and a curious asymmetry in the ORD of pB(+)p versus the ORD of pB(-)p is reported. Furthermore, it is shown that (+)-p-menth-1-ene and (-)-p-menth-1-ene are useful as a model compounds for the pBp resins and for the explanation of the amplification of the optical activity of the {beta}(+)-pinene and {beta}(-)-pinene after their ring-opening polymerization to pB(+)p and pB(-)p. The pBp resins were studied also by FT-IR spectroscopy and by thermal analysis (TGA and DTG).

  11. Evidence for Novel [beta]-Sheet Structures in Iowa Mutant [beta]-Amyloid Fibrils

    Energy Technology Data Exchange (ETDEWEB)

    Tycko, Robert; Sciarretta, Kimberly L.; Orgel, Joseph P.R.O.; Meredith, Stephen C.; (IIT); (NIH); (UC)


    Asp23-to-Asn mutation within the coding sequence of {beta}-amyloid, called the Iowa mutation, is associated with early onset, familial Alzheimer's disease and cerebral amyloid angiopathy, in which patients develop neuritic plaques and massive vascular deposition predominantly of the mutant peptide. We examined the mutant peptide, D23N-A{beta}40, by electron microscopy, X-ray diffraction, and solid-state NMR spectroscopy. D23N-A{beta}40 forms fibrils considerably faster than the wild-type peptide (k = 3.77 x 10{sup -3} min{sup -1} and 1.07 x 10{sup -4} min{sup -1} for D23N-A{beta}40 and the wild-type peptide WT-A{beta}40, respectively) and without a lag phase. Electron microscopy shows that D23N-A{beta}40 forms fibrils with multiple morphologies. X-ray fiber diffraction shows a cross-{beta} pattern, with a sharp reflection at 4.7 {angstrom} and a broad reflection at 9.4 {angstrom}, which is notably smaller than the value for WT-A{beta}40 fibrils (10.4 {angstrom}). Solid-state NMR measurements indicate molecular level polymorphism of the fibrils, with only a minority of D23N-A{beta}40 fibrils containing the in-register, parallel {beta}-sheet structure commonly found in WT-A{beta}40 fibrils and most other amyloid fibrils. Antiparallel {beta}-sheet structures in the majority of fibrils are indicated by measurements of intermolecular distances through 13C-13C and 15N-13C dipole-dipole couplings. An intriguing possibility exists that there is a relationship between the aberrant structure of D23N-A{beta}40 fibrils and the unusual vasculotropic clinical picture in these patients.

  12. Beta-carotene (United States)

    ... patches on the tongue and mouth called oral leukoplakia. Taking beta-carotene by mouth for up to 12 months seems to decrease symptoms of oral leukoplakia. Osteoarthritis. Beta-carotene taken by mouth may prevent ...

  13. Forward-Looking Betas

    DEFF Research Database (Denmark)

    Christoffersen, Peter; Jacobs, Kris; Vainberg, Gregory

    Few issues are more important for finance practice than the computation of market betas. Existing approaches compute market betas using historical data. While these approaches differ in terms of statistical sophistication and the modeling of the time-variation in the betas, they are all backward......-looking. This paper introduces a radically different approach to estimating market betas. Using the tools in Bakshi and Madan (2000) and Bakshi, Kapadia and Madan (2003) we employ the information embedded in the prices of individual stock options and index options to compute our forward-looking market beta...

  14. Design, synthesis, and evaluation of 2 beta-alkenyl penam sulfone acids as inhibitors of beta-lactamases. (United States)

    Richter, H G; Angehrn, P; Hubschwerlen, C; Kania, M; Page, M G; Specklin, J L; Winkler, F K


    A general method for synthesis of 2 beta-alkenyl penam sulfones has been developed. The new compounds inhibited most of the common types of beta-lactamase. The level of activity depended very strongly on the nature of the substituent in the 2 beta-alkenyl group. The inhibited species formed with the beta-lactamase from Citrobacter freundii 1205 was sufficiently stable for X-ray crystallographic studies. These, together with UV absorption spectroscopy and studies of chemical degradation, suggested a novel reaction mechanism for the new inhibitors that might account for their broad spectrum of action. The (Z)-2 beta-acrylonitrile penam sulfone Ro 48-1220 was the most active inhibitor from this class of compound. The inhibitor enhanced the action of, for example, ceftriaxone against a broad selection of organisms producing beta-lactamases. The organisms included strains of Enterobacteriaceae that produce cephalosporinases, which is an exceptional activity for penam sulfones.

  15. Conformation, molecular packing and field effect mobility of regioregular beta,beta'-dihexylsexithiophiophene

    DEFF Research Database (Denmark)

    Kiriy, N.; Kiriy, A.; Bocharova, V.


    by the pulse-radiolysis time-resolved microwave conductivity (PR-TRMC) technique was found to be Sigmamu(min) = 3.9 x 10(-3) cm(2) V-1 s(-1), which is comparable with the PR-TRMC mobility found for alpha,omega-DH6T. The field-effect mobility (FEM) of beta,beta'-DH6T was found to be on the order of 10(-5) cm(2......) V-1 s(-1), which is considerably less than the FEM of alpha,omega-DH6T. To understand the reason for such poor macroscopic electrical properties, the conformation and the molecular packing of beta,beta'-DH6T were systematically studied by means of UV-vis spectroscopy, scanning electron microscopy...

  16. Beta decay of {sup 61}Ga

    Energy Technology Data Exchange (ETDEWEB)

    Oinonen, M.; Dendooven, P.; Jokinen, A.; Penttilae, H.; Aeystoe, J. [Jyvaeskylae Univ. (Finland). Dept. of Physics; Baumann, P.; Knipper, A.; Ramdhane, M.; Walter, G. [Institut de Recherches Subatomiques, F-67037 Strasbourg (France); Fujita, Y. [Department of Physics, Osaka University, Toyonaka, Osaka 560-0043 (Japan); Gorska, M.; Grawe, H.; Hu, Z.; Kirchner, R.; Klepper, O.; Liu, W.; Roeckl, E. [Gesellschaft fuer Schwerionenforschung, Postfach 110552, D-64220 Darmstadt (Germany); Janas, Z.; Plochocki, A. [Institute of Experimental Physics, Warsaw University, PL-00681 Warsaw (Poland)


    The {beta} decay of {sup 61}Ga to its mirror nucleus {sup 61}Zn has been measured for the first time by using on-line mass separation and {beta}-delayed gamma-ray spectroscopy. The observed decay strength to the ground state implies superallowed character in accordance with the systematics of the mirror decays in the sd and fp shell. The {beta} feedings observed to four excited states in {sup 61}Zn are consistent with earlier spin-parity assignments based on in-beam experiments. The ground-state spin and parity for {sup 61}Ga were determined to be 3/2{sup -}. (orig.) With 3 figs., 2 tabs., 37 refs.

  17. Betting against Beta

    DEFF Research Database (Denmark)

    Frazzini, Andrea; Heje Pedersen, Lasse


    We present a model with leverage and margin constraints that vary across investors and time. We find evidence consistent with each of the model's five central predictions: (1) Because constrained investors bid up high-beta assets, high beta is associated with low alpha, as we find empirically for......, the return of the BAB factor is low. (4) Increased funding liquidity risk compresses betas toward one. (5) More constrained investors hold riskier assets....... for US equities, 20 international equity markets, Treasury bonds, corporate bonds, and futures. (2) A betting against beta (BAB) factor, which is long leveraged low-beta assets and short high-beta assets, produces significant positive risk-adjusted returns. (3) When funding constraints tighten...

  18. Betting Against Beta

    DEFF Research Database (Denmark)

    Frazzini, Andrea; Heje Pedersen, Lasse

    We present a model with leverage and margin constraints that vary across investors and time. We find evidence consistent with each of the model’s five central predictions: (1) Since constrained investors bid up high-beta assets, high beta is associated with low alpha, as we find empirically for U...... of the BAB factor is low; (4) Increased funding liquidity risk compresses betas toward one; (5) More constrained investors hold riskier assets........S. equities, 20 international equity markets, Treasury bonds, corporate bonds, and futures; (2) A betting-against-beta (BAB) factor, which is long leveraged low beta assets and short high-beta assets, produces significant positive risk-adjusted returns; (3) When funding constraints tighten, the return...

  19. Imperfect World of $\\beta\\beta$-decay Nuclear Data Sets

    CERN Document Server

    Pritychenko, B


    The precision of double-beta ($\\beta\\beta$) decay experimental half lives and their uncertainties is reanalyzed. The method of Benford's distributions has been applied to nuclear reaction, structure and decay data sets. First-digit distribution trend for $\\beta\\beta$-decay T$_{1/2}^{2\

  20. Terahertz spectroscopy

    DEFF Research Database (Denmark)

    Jepsen, Peter Uhd


    In this presentation I will review methods for spectroscopy in the THz range, with special emphasis on the practical implementation of the technique known ad THz time-domain spectroscopy (THz-TDS). THz-TDS has revived the old field of far-infrared spectroscopy, and enabled a wealth of new...

  1. An interaction of beta-amyloid with aluminium in vitro. (United States)

    Exley, C; Price, N C; Kelly, S M; Birchall, J D


    We have used circular dichroism spectroscopy to confirm that, in a membrane-mimicking solvent, A beta P(1-40) adopts a partially helical conformation and we have demonstrated the loss of this structure in the presence of physiologically relevant concentrations of aluminium. This is the first evidence of a direct biochemical interaction between aluminium and beta-amyloid and may have important implications for the pathogenesis of Alzheimer's disease.

  2. Beta decay of {sup 103}Sn

    Energy Technology Data Exchange (ETDEWEB)

    Kavatsyuk, O.; Kavatsyuk, M. [GSI, Darmstadt (Germany); National Taras Shevcjenko Univ. of Kyiv (Australia); Batist, L. [St. Petersburg Nuclear Physics Inst. (Russian Federation); Univ. di Napoli (Italy); INFN, Napoli (Italy); Banu, A.; Becker, F.; Bruechle, W.; Doering, J.; Gorska, M.; Grawe, H.; Kirchner, R.; Mandal, S.; Mazzocchi, C.; Plettner, C.; Roeckl, E.; Schaedel, M. [GSI, Darmstadt (Germany); Blazhev, A. [GSI, Darmstadt (Germany); Univ. Sofia (Bulgaria); Faestermann, T. [Technische Universitaet Muenchen (Germany); Janas, Z.; Karny, M.; Plochocki, A.; Zylicz, J. [University of Warsaw (Poland); Jungclaus, A. [Universidad Autonoma de Madrid, Departamento de Fisica Teorica (Spain); La Commara, M.; Romoli, M. [INFN, Napoli (Italy); Mukha, I. [GSI, Darmstadt (Germany); Kurchatov Inst. Moscow (Russian Federation); Muralithar, S. [GSI, Darmstadt (Germany); Forschungszentrum Rossendorf (Germany); Schwengner, R. [Forschungszentrum Rossendorf (Germany)


    The {beta} decay of {sup 103}Sn, a three-neutron-particle nucleus with respect to the {sup 100}Sn core, was investigated at the GSI on-line mass separator using an array of 17 germanium crystals and a total absorption spectrometer. A total of 31 {beta}-delayed {gamma}-rays (29 new) of the {sup 103}Sn{yields}{sup 103}In decay were observed and, on the basis of {beta}-{gamma}-{gamma} coincidences, the {sup 103}Sn decay scheme was established for the first time. By means of total absorption spectroscopy, {beta} intensities, the Gamow-Teller strength distribution and the summed Gamow-Teller strength value of 3.5{+-}0.5 were determined for this decay. Its half-life and Q{sub EC} value were found to be 7.0{+-}0.2 s and 7.64{+-}0.7 MeV, respectively. The {beta}-delayed proton branching ratio was measured to be 1.2{+-}0.1%. The results are discussed in comparison with shell-model predictions based on realistic and empirical interactions. (orig.)

  3. NMR study of the preparation of 6 {alpha}, 7 {beta}-dihydroxyvouacapan-17 beta-oic acid mannich base derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Flavio Jose Leite dos; Pilo-Veloso, Dorila [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte (Brazil). Inst. de Ciencias Exatas. Dept. Quimica]. E-mail:; Ferreira-Alves, Dalton L. [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte (Brazil). Inst. de Ciencias Biologicas. Dept. de Farmacologia


    This work presents four new Mannich base compounds obtained by the Mannich reaction of a {delta}-keto-lactone derivative of 6{alpha}, 7{beta}-dihydroxyvouacapan- 17{beta}-oic acid, a furano diterpene isolated from the hexane extract of Pterodon polygalaeflorus Benth fruits, which shows anti-inflammatory and analgesic activities. The use of 1D and 2D NMR (COSY, DEPT-135, HMBC, HMQC) spectroscopy made it possible to characterize the new compounds. (author)

  4. Negative Beta Encoder

    CERN Document Server

    Kohda, Tohru; Aihara, Kazuyuki


    A new class of analog-digital (A/D), digital-analog (D/A) converters as an alternative to conventional ones, called $\\beta$-encoder, has been shown to have exponential accuracy in the bit rates while possessing self-correction property for fluctuations of amplifier factor $\\beta$ and quantizer threshold $\

  5. Double beta decay experiments

    CERN Document Server

    Barabash, A S


    The present status of double beta decay experiments is reviewed. The results of the most sensitive experiments are discussed. Proposals for future double beta decay experiments with a sensitivity to the $$ at the level of (0.01--0.1) eV are considered.

  6. Genetics Home Reference: beta thalassemia (United States)

    ... Understand Genetics Home Health Conditions beta thalassemia beta thalassemia Enable Javascript to view the expand/collapse boxes. Download PDF Open All Close All Description Beta thalassemia is a blood disorder that reduces the production ...

  7. Rapid synthesis of beta zeolites (United States)

    Fan, Wei; Chang, Chun -Chih; Dornath, Paul; Wang, Zhuopeng


    The invention provides methods for rapidly synthesizing heteroatom containing zeolites including Sn-Beta, Si-Beta, Ti-Beta, Zr-Beta and Fe-Beta. The methods for synthesizing heteroatom zeolites include using well-crystalline zeolite crystals as seeds and using a fluoride-free, caustic medium in a seeded dry-gel conversion method. The Beta zeolite catalysts made by the methods of the invention catalyze both isomerization and dehydration reactions.

  8. Comparative study on the inclusion behavior between meso-tetrakis(4-N-ethylpyridiniurmyl)porphyrin and beta-cyclodextrin derivatives. (United States)

    Xiliang, Guo; Shaomin, Shuang; Chuan, Dong; Feng, Feng; Wong, M S


    5,10,15,20-Tetrakis(4-N-ethylpyridiniurmyl)porphyrin (TEPyP) formed 1:1 stoichiometry inclusion complexes with beta-cyclodextrin (beta-CD) and its derivatives including hydroxypropyl-beta-cyclodextrin (HP-beta-CD), sulfobutylether-beta-cyclodextrin (SBE-beta-CD) in basic aqueous solution. The supramolecular system was investigated by the methods of fluorescence, UV-vis absorption spectroscopy, nuclear magnetic resonance (NMR) technique. The inclusion ability of cyclodextrins exhibited remarkable difference for beta-CD, HP-beta-CD and SBE-beta-CD. Association constants as high as K=1.1 x 10(4) M(-1) in the case of HP-beta-CD/TEPyP and 2.0 x 10(5) M(-1) in the case of SBE-beta-CD/TEPyP complexes were determined, whereas a lower value (K=550 M(-1)) was given in the case of beta-CD/TEPyP. The results showed that hydrogen bonding and charge attraction play important roles in the processes of host-guest interaction. The interaction mechanism of inclusion processes could be explained by the analysis of NMR spectroscopy. The supramolecular assembly was formed. beta-CD and HP-beta-CD approached from the primary face of cavities of CDs.

  9. The iA{beta}5p {beta}-breaker peptide regulates the A{beta}(25-35) interaction with lipid bilayers through a cholesterol-mediated mechanism

    Energy Technology Data Exchange (ETDEWEB)

    Vitiello, Giuseppe [Department of Chemistry, University of Naples ' Federico II' , Naples (Italy); CSGI (Consorzio per lo Sviluppo dei Sistemi a Grande Interfase), Florence (Italy); Grimaldi, Manuela; D' Ursi, Anna Maria [Department of Pharmaceutical Science, University of Salerno, Fisciano (Italy); D' Errico, Gerardino, E-mail: [Department of Chemistry, University of Naples ' Federico II' , Naples (Italy); CSGI (Consorzio per lo Sviluppo dei Sistemi a Grande Interfase), Florence (Italy)


    Highlights: Black-Right-Pointing-Pointer iA{beta}5p shows a significant tendency to deeply penetrates the hydrophobic core of lipid membrane. Black-Right-Pointing-Pointer A{beta}(25-35) locates in the external region of the membrane causing a re-positioning of CHOL. Black-Right-Pointing-Pointer iA{beta}5p withholds cholesterol in the inner hydrophobic core of the lipid membrane. Black-Right-Pointing-Pointer iA{beta}5p prevents the A{beta}(25-35) release from the lipid membrane. -- Abstract: Alzheimer's disease is characterized by the deposition of aggregates of the {beta}-amyloid peptide (A{beta}) in the brain. A potential therapeutic strategy for Alzheimer's disease is the use of synthetic {beta}-sheet breaker peptides, which are capable of binding A{beta} but unable to become part of a {beta}-sheet structure, thus inhibiting the peptide aggregation. Many studies suggest that membranes play a key role in the A{beta} aggregation; consequently, it is strategic to investigate the interplay between {beta}-sheet breaker peptides and A{beta} in the presence of lipid bilayers. In this work, we focused on the effect of the {beta}-sheet breaker peptide acetyl-LPFFD-amide, iA{beta}5p, on the interaction of the A{beta}(25-35) fragment with lipid membranes, studied by Electron Spin Resonance spectroscopy, using spin-labeled membrane components (either phospholipids or cholesterol). The ESR results show that iA{beta}5p influences the A{beta}(25-35) interaction with the bilayer through a cholesterol-mediated mechanism: iA{beta}5p withholds cholesterol in the inner hydrophobic core of the bilayer, making the interfacial region more fluid and capable to accommodate A{beta}(25-35). As a consequence, iA{beta}5p prevents the A{beta}(25-35) release from the lipid membrane, which is the first step of the {beta}-amyloid aggregation process.

  10. Preparation and spectral investigation of inclusion complex of caffeic acid with hydroxypropyl-beta-cyclodextrin. (United States)

    Zhang, Min; Li, Jinxia; Zhang, Liwei; Chao, Jianbin


    The inclusion complexation behavior of caffeic acid (CA) with hydroxypropyl-beta-cyclodextrin (HP-beta-CD) was studied by UV-vis, fluorescence spectroscopy and nuclear magnetic resonance spectroscopy (NMR). Experimental conditions including the concentration of HP-beta-CD and media acidity were investigated in detail. The result suggested HP-beta-CD was more suitable for including CA in acidity solution. The binding contants (K) of the inclusion complexes were determined by linear regression analysis and the inclusion ratio was found to be 1:1. The water solubility of CA was increased by inclusion with HP-beta-CD according to the phase-solubility diagram. The spatial configuration of complex has been proposed based on (1)H NMR and two-dimensional (2D) NMR, the result suggested that CA was entrapped inside the hydrophobic core of HP-beta-CD with the lipophilic aromatic ring and the portion of ethylene.

  11. Cloning of beta-primeverosidase from tea leaves, a key enzyme in tea aroma formation. (United States)

    Mizutani, Masaharu; Nakanishi, Hidemitsu; Ema, Jun-ichi; Ma, Seung-Jin; Noguchi, Etsuko; Inohara-Ochiai, Misa; Fukuchi-Mizutani, Masako; Nakao, Masahiro; Sakata, Kanzo


    A beta-primeverosidase from tea (Camellia sinensis) plants is a unique disaccharide-specific glycosidase, which hydrolyzes aroma precursors of beta-primeverosides (6-O-beta-D-xylopyranosyl-beta-D-glucopyranosides) to liberate various aroma compounds, and the enzyme is deeply concerned with the floral aroma formation in oolong tea and black tea during the manufacturing process. The beta-primeverosidase was purified from fresh leaves of a cultivar for green tea (C. sinensis var sinensis cv Yabukita), and its partial amino acid sequences were determined. The beta-primeverosidase cDNA has been isolated from a cDNA library of cv Yabukita using degenerate oligonucleotide primers. The cDNA insert encodes a polypeptide consisting of an N-terminal signal peptide of 28 amino acid residues and a 479-amino acid mature protein. The beta-primeverosidase protein sequence was 50% to 60% identical to beta-glucosidases from various plants and was classified in a family 1 glycosyl hydrolase. The mature form of the beta-primeverosidase expressed in Escherichia coli was able to hydrolyze beta-primeverosides to liberate a primeverose unit and aglycons, but did not act on 2-phenylethyl beta-D-glucopyranoside. These results indicate that the beta-primeverosidase selectively recognizes the beta-primeverosides as substrates and specifically hydrolyzes the beta-glycosidic bond between the disaccharide and the aglycons. The stereochemistry for enzymatic hydrolysis of 2-phenylethyl beta-primeveroside by the beta-primeverosidase was followed by (1)H-nuclear magnetic resonance spectroscopy, revealing that the enzyme hydrolyzes the beta-primeveroside by a retaining mechanism. The roles of the beta-primeverosidase in the defense mechanism in tea plants and the floral aroma formation during tea manufacturing process are also discussed.

  12. Neutrinoless double beta decay

    Indian Academy of Sciences (India)

    Kai Zuber


    The physics potential of neutrinoless double beta decay is discussed. Furthermore, experimental considerations as well as the current status of experiments are presented. Finally, an outlook towards the future, work on nuclear matrix elements and alternative processes is given.

  13. Mechanistic Insight in the Enzymatic Ring-Opening Polymerization of beta-Propiolactam

    NARCIS (Netherlands)

    Schwab, Leendert W.; Baum, Iris; Fels, Gregor; Loos, Katja; Cheng, HN; Gross, RA


    Here we report the polymerization of beta-propiolactam to poly(beta-alanine) catalyzed by the immobilized Candida antarctica lipase B (N435). The polymer is characterized by (1)H-NMR spectroscopy and MALDI-ToF mass spectrometry. The best results were obtained with N435 (dried for 24 hours in vacua a

  14. Alpha and Beta Determinations

    CERN Document Server

    Dunietz, Isard


    Because the Bd -> J/psi Ks asymmetry determines only sin(2 beta), a discrete ambiguity in the true value of beta remains. This note reviews how the ambiguity can be removed. Extractions of the CKM angle alpha are discussed next. Some of the methods require very large data samples and will not be feasible in the near future. In the near future, semi-inclusive CP-violating searches could be undertaken, which are reviewed last.

  15. {beta} - amyloid imaging probes

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Jae Min [Seoul National University College of Medicine, Seoul (Korea, Republic of)


    Imaging distribution of {beta} - amyloid plaques in Alzheimer's disease is very important for early and accurate diagnosis. Early trial of the {beta} -amyloid plaques includes using radiolabeled peptides which can be only applied for peripheral {beta} - amyloid plaques due to limited penetration through the blood brain barrier (BBB). Congo red or Chrysamine G derivatives were labeled with Tc-99m for imaging {beta} - amyloid plaques of Alzheimer patient's brain without success due to problem with BBB penetration. Thioflavin T derivatives gave breakthrough for {beta} - amyloid imaging in vivo, and a benzothiazole derivative [C-11]6-OH-BTA-1 brought a great success. Many other benzothiazole, benzoxazole, benzofuran, imidazopyridine, and styrylbenzene derivatives have been labeled with F-18 and I-123 to improve the imaging quality. However, [C-11]6-OH-BTA-1 still remains as the best. However, short half-life of C-11 is a limitation of wide distribution of this agent. So, it is still required to develop an Tc-99m, F-18 or I-123 labeled agent for {beta} - amyloid imaging agent.

  16. Beta cell dynamics: beta cell replenishment, beta cell compensation and diabetes. (United States)

    Cerf, Marlon E


    Type 2 diabetes, characterized by persistent hyperglycemia, arises mostly from beta cell dysfunction and insulin resistance and remains a highly complex metabolic disease due to various stages in its pathogenesis. Glucose homeostasis is primarily regulated by insulin secretion from the beta cells in response to prevailing glycemia. Beta cell populations are dynamic as they respond to fluctuating insulin demand. Beta cell replenishment and death primarily regulate beta cell populations. Beta cells, pancreatic cells, and extra-pancreatic cells represent the three tiers for replenishing beta cells. In rodents, beta cell self-replenishment appears to be the dominant source for new beta cells supported by pancreatic cells (non-beta islet cells, acinar cells, and duct cells) and extra-pancreatic cells (liver, neural, and stem/progenitor cells). In humans, beta cell neogenesis from non-beta cells appears to be the dominant source of beta cell replenishment as limited beta cell self-replenishment occurs particularly in adulthood. Metabolic states of increased insulin demand trigger increased insulin synthesis and secretion from beta cells. Beta cells, therefore, adapt to support their physiology. Maintaining physiological beta cell populations is a strategy for targeting metabolic states of persistently increased insulin demand as in diabetes.

  17. Synthesis, spectroscopy (IR, multinuclear NMR, ESI-MS), diffraction, density functional study and in vitro antiproliferative activity of pyrazole-beta-diketone dihalotin(IV) compounds on 5 melanoma cell lines. (United States)

    Pettinari, Claudio; Caruso, Francesco; Zaffaroni, Nadia; Villa, Raffaella; Marchetti, Fabio; Pettinari, Riccardo; Phillips, Christine; Tanski, Joseph; Rossi, Miriam


    Novel 4-acylpyrazolon-5-ato-dihalotin(IV) complexes, [Q2SnX2], (X = F, Cl, Br or I); HQ = HQ(CHPh2) (1,2-dihydro-3-methyl-1-phenyl-4-(2,2-diphenylacetyl)pyrazol-5-one), HQ(Bn) (1,2-dihydro-3-methyl-1-phenyl-4-(2-phenylacetyl)pyrazol-5-one) or HQ(CF3,py) (4-(2,2,2-trifluoroacetyl)-1,2-dihydro-3-methyl-1-(pyridin-2-yl)pyrazol-5-one) have been synthesized and characterized by spectroscopic (IR, 1H, 13C, 19F and 119Sn NMR, electrospray ionisation mass spectrometry (ESI-MS)), analytical and structural methods (X-ray and density functional theory). 119Sn chemical shifts depend on the nature of the halides bonded to tin. Isomer conversion, detected in solution by NMR spectroscopy, is related to the acyl moiety bulkiness while the cis(Cl)-cis(acyl)-trans(pyrazolonato) scheme is found in the solid state. The in vitro antiproliferative tests of three derivatives on three human melanoma cell lines (JR8, SK-MEL-5, MEL501) and two melanoma cell clones (2/21 and 2/60) show dose-dependent decrease of cell proliferation in all cell lines. The activity correlates with the nature of the substituent on position 1 of pyrazole, decreasing in the order pyridyl>Ph>methyl. The activity for (Q(CF3,py))2SnCl2 on the SK-MEL-5 cell line is IC50 = 50 microM.

  18. Beta-decay properties of Si-25 and P-26

    NARCIS (Netherlands)

    Achouri, L; Aysto, J; Beraud, R; Blank, B; Canchel, G; Czajkowski, S; Dendooven, P; Ensallem, A; Giovinazzo, J; Guillet, N; Honkanen, J; Jokinen, A; Laird, A; Lewitowicz, M; Longour, C; Santos, FD; Perajarvi, K; Stanoiu, M


    The beta-decay properties of the neutron-deficient nuclei Si-25 and P-26 have been investigated at the GANIL/LISE3 facility by means of charged-particle and gamma-ray spectroscopy. The decay schemes obtained and the Gamow-Teller strength distributions are compared to shell-model calculations based o

  19. Laser spectroscopy

    CERN Document Server

    Demtröder, Wolfgang

    Keeping abreast of the latest techniques and applications, this new edition of the standard reference and graduate text on laser spectroscopy has been completely revised and expanded. While the general concept is unchanged, the new edition features a broad array of new material, e.g., ultrafast lasers (atto- and femto-second lasers) and parametric oscillators, coherent matter waves, Doppler-free Fourier spectroscopy with optical frequency combs, interference spectroscopy, quantum optics, the interferometric detection of gravitational waves and still more applications in chemical analysis, medical diagnostics, and engineering.

  20. Preparation and electrochemical behavior of water-soluble inclusion complex of ferrocene with {beta}-cyclodextrin polymer

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Wang; Chen Ming [College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, Jiangsu (China); Diao Guowang, E-mail: [College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, Jiangsu (China)


    Highlights: > Water-soluble Fc-{beta}-CD polymer inclusion complex is prepared with a supermolecular method. > Fc-{beta}-CDP shows better aqueous solubility remarkably than Fc and Fc-{beta}-CD. > It also reserves the electrochemical properties of Fc-{beta}-CDP in aqueous solution. > It is determined the electrochemical constants and dissociated constant. > The method opens up aqueous applications of insoluble organic compounds in electrochemistry. - Abstract: A new water-soluble inclusion complex of ferrocene (Fc) with {beta}-cyclodextrin polymer ({beta}-CDP) was prepared by a facile strategy and characterized by {sup 1}H NMR spectroscopy, elemental analysis, powder X-ray diffractometry, thermogravimetry, UV-vis spectroscopy and cyclic voltammetry. Compared with Fc and the inclusion complex of Fc with {beta}-cyclodextrin (Fc-{beta}-CD), the solubility of ferrocene-{beta}-cyclodextrin polymer (Fc-{beta}-CDP) was greatly enhanced due to the water-soluble {beta}-CDP host. The ratio of {beta}-cyclodextrin ({beta}-CD) unit in {beta}-CDP to Fc was determined as 1:1. At 25 deg. C, the dissociated constant of Fc-{beta}-CDP was measured as 3.65 mM by UV-vis spectroscopy and cyclic voltammetry. The electrochemical properties of Fc-{beta}-CDP in water were studied. The diffusion coefficients of oxidation state and reduction state were calculated as 3.52 x 10{sup -7} cm{sup 2} s{sup -1} and 3.93 x 10{sup -7} cm{sup 2} s{sup -1}. The resulting value of standard rate constant was measured as 1.95 x 10{sup -3} cm s{sup -1}. The diffusion activation energy was calculated as 21.8 kJ mol{sup -1}.

  1. Boosted beta regression.

    Directory of Open Access Journals (Sweden)

    Matthias Schmid

    Full Text Available Regression analysis with a bounded outcome is a common problem in applied statistics. Typical examples include regression models for percentage outcomes and the analysis of ratings that are measured on a bounded scale. In this paper, we consider beta regression, which is a generalization of logit models to situations where the response is continuous on the interval (0,1. Consequently, beta regression is a convenient tool for analyzing percentage responses. The classical approach to fit a beta regression model is to use maximum likelihood estimation with subsequent AIC-based variable selection. As an alternative to this established - yet unstable - approach, we propose a new estimation technique called boosted beta regression. With boosted beta regression estimation and variable selection can be carried out simultaneously in a highly efficient way. Additionally, both the mean and the variance of a percentage response can be modeled using flexible nonlinear covariate effects. As a consequence, the new method accounts for common problems such as overdispersion and non-binomial variance structures.

  2. Fluorescence spectroscopy

    DEFF Research Database (Denmark)

    Bagatolli, Luis


    Fluorescence spectroscopy is a powerful experimental tool used by scientists from many disciplines. During the last decades there have been important developments on distinct fluorescence methods, particularly those related to the study of biological phenomena. This chapter discusses the foundati......Fluorescence spectroscopy is a powerful experimental tool used by scientists from many disciplines. During the last decades there have been important developments on distinct fluorescence methods, particularly those related to the study of biological phenomena. This chapter discusses...

  3. Beta and Gamma Gradients

    DEFF Research Database (Denmark)

    Løvborg, Leif; Gaffney, C. F.; Clark, P. A.;


    Experimental and/or theoretical estimates are presented concerning, (i) attenuation within the sample of beta and gamma radiation from the soil, (ii) the gamma dose within the sample due to its own radioactivity, and (iii) the soil gamma dose in the proximity of boundaries between regions...... of differing radioactivity. It is confirmed that removal of the outer 2 mm of sample is adequate to remove influence from soil beta dose and estimates are made of the error introduced by non-removal. Other evaluations include variation of the soil gamma dose near the ground surface and it appears...

  4. Applied Beta Dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    Rich, B.L.


    Measurements of beta and/or nonpenetrating exposure results is complicated and past techniques and capabilities have resulted in significant inaccuracies in recorded results. Current developments have resulted in increased capabilities which make the results more accurate and should result in less total exposure to the work force. Continued development of works in progress should provide equivalent future improvements.

  5. Beta Thalassemia (For Parents) (United States)

    ... had their spleens removed. Slower growth rates. The anemia resulting from beta thalassemia can cause children to grow more slowly and also can lead ... boost production of new red blood cells. Some children with moderate anemia may require an occasional blood transfusion , particularly after ...

  6. Trichoderma .beta.-glucosidase (United States)

    Dunn-Coleman, Nigel; Goedegebuur, Frits; Ward, Michael; Yao, Jian


    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl3, and the corresponding BGL3 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL3, recombinant BGL3 proteins and methods for producing the same.

  7. Roughing up Beta

    DEFF Research Database (Denmark)

    Bollerslev, Tim; Li, Sophia Zhengzi; Todorov, Viktor

    Motivated by the implications from a stylized equilibrium pricing framework, we investigate empirically how individual equity prices respond to continuous, or \\smooth," and jumpy, or \\rough," market price moves, and how these different market price risks, or betas, are priced in the cross-section...

  8. Electron Spectroscopy (United States)

    Siegbahn, Kai

    Wilhelm Conrad Röntgen's discovery of X radiation in 1895 in Wörzburg resulted in an immediate break-through not only in physics but also in Society, the latter mainly because of its sensational radiological applications. Within a short time it furthermore indirectly led to the discovery of radioactivity by Henri Becquerel. The discovery of X radiation opened the gate to modern atomic physics, and radioactivity to nuclear physics. Later on, the discovery of X-ray diffraction by Laue, Friedrich and Knipping in 1912 initiated the field of X-ray spectroscopy with its fundamental contributions to atomic and crystal structures. Secondary electrons were early observed in the scattered radiation when X-rays were hitting a sample. The development of the corresponding electron spectroscopy had to wait a much longer time for its maturity. A survey of electron spectroscopy is presented.

  9. Radiation synthesis of gelatin/CM-chitosan/{beta}-tricalcium phosphate composite scaffold for bone tissue engineering

    Energy Technology Data Exchange (ETDEWEB)

    Zhou Ying [College of Engineering, Peking University, Beijing 100871 (China); Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871 (China); Xu Ling, E-mail: [College of Engineering, Peking University, Beijing 100871 (China); Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871 (China); Zhang Xiangmei; Zhao Yinghui [Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871 (China); Wei Shicheng, E-mail: [Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871 (China); Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing 100081 (China); Zhai Maolin [Beijing National Laboratory for Molecular Sciences, Department of Applied Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China)


    A series of biodegradable composite scaffolds was fabricated from an aqueous solution of gelatin, carboxymethyl chitosan (CM-chitosan) and {beta}-tricalcium phosphate ({beta}-TCP) by radiation-induced crosslinking at ambient temperature. Ultrasonic treatment on the polymer solutions significantly influenced the distribution of {beta}-TCP particles. An ultrasonic time of 20 min, followed by 30 kGy irradiation induced a crosslinked scaffold with homogeneous distribution of {beta}-TCP particles, interconnected porous structure, sound swelling capacity and mechanical strength. Fourier Transform Infrared Spectroscopy and X-ray Diffraction analysis indicated that {beta}-TCP successfully incorporated with the network of gelatin and CM-chitosan. In vivo implantation of the scaffold into the mandible of beagle dog revealed that the scaffolds had excellent biocompatibility and the presence of {beta}-TCP can accelerate bone regeneration. The comprehensive results of this study paved way for the application of gelatin/CM-chitosan/{beta}-TCP composite scaffolds as candidate of bone tissue engineering material. - Highlights: Black-Right-Pointing-Pointer Radiation induced a crosslinked scaffold with interconnected porous structure. Black-Right-Pointing-Pointer Ultrasonic time of 20 min led to homogenerously distribution of {beta}-TCP. Black-Right-Pointing-Pointer Increasing amount of {beta}-TCP would restrict the swelling properties. Black-Right-Pointing-Pointer Proper fraction of {beta}-TCP will promote the mechanical properties of the scaffolds. Black-Right-Pointing-Pointer Hybrid of {beta}-TCP promoted the bone regeneration of the mandibles of beagle dogs.

  10. TGF-beta and osteoarthritis.

    NARCIS (Netherlands)

    Blaney Davidson, E.N.; Kraan, P.M. van der; Berg, W.B. van den


    OBJECTIVE: Cartilage damage is a major problem in osteoarthritis (OA). Growth factors like transforming growth factor-beta (TGF-beta) have great potential in cartilage repair. In this review, we will focus on the potential therapeutic intervention in OA with TGF-beta, application of the growth facto

  11. The Dust and Gas Around beta Pictoris

    CERN Document Server

    Chen, C H; Bohac, C; Kim, K H; Watson, D M; van Cleve, J; Houck, J; Stapelfeldt, K; Werner, M W; Rieke, G; Su, K; Marengo, M; Backman, D; Beichman, C; Fazio, G


    We have obtained Spitzer IRS 5.5 - 35 micron spectroscopy of the debris disk around beta Pictoris. In addition to the 10 micron silicate emission feature originally observed from the ground, we also detect the crystalline silicate emission bands at 28 micron and 33.5 micron. This is the first time that the silicate bands at wavelengths longer than 10 micron have ever been seen in the beta Pictoris disk. The observed dust emission is well reproduced by a dust model consisting of fluffy cometary and crystalline olivine aggregates. We searched for line emission from molecular hydrogen and atomic [S I], Fe II, and Si II gas but detected none. We place a 3 sigma upper limit of <17 Earth masses on the H2 S(1) gas mass, assuming an excitation temperature of Tex = 100 K. This suggests that there is less gas in this system than is required to form the envelope of Jupiter. We hypothesize that some of the atomic Na I gas observed in Keplerian rotation around beta Pictoris may be produced by photon-stimulated desorpti...

  12. Beta decay of {sup 101}Sn

    Energy Technology Data Exchange (ETDEWEB)

    Kavatsyuk, O.; Kavatsyuk, M. [Gesellschaft fuer Schwerionenforschung, Damrstadt (Germany); National Taras Shevchenko Univ. of Kyiv (Ukraine); Mazzocchi, C. [Gesellschaft fuer Schwerionenforschung, Damrstadt (Germany); Univ. of Tennessee, Knoxville (United States); Janas, Z.; Karny, M.; Miernik, K.; Plochocki, A.; Zylicz, J. [University of Warsaw (Poland); Banu, A.; Becker, F.; Bruechle, W.; Doering, J.; Gorska, M.; Grawe, H.; Klepper, O.; Kirchner, R.; Plettner, C.; Roeckl, E.; Schaedel, M. [Gesellschaft fuer Schwerionenforschung, Darmstadt, Darmstadt (Germany); Batist, L. [St. Petersburg Nuclear Physics Institute (Russian Federation); Blazhev, A. [Gesellschaft fuer Schwerionenforschung, Damrstadt (Germany); Univ. of Sofia (Bulgaria); Faestermann, T. [Technische Universitaet Muenchen (Germany); Jungclaus, A. [Instituto Estructura de la Materia, CSIC (Spain); Departamento de Fisica Teorica, UAM, Madrid (Spain); La Commara, M.; Romoli, M. [Universita ' ' Federico II' ' , Napoli (Italy); INFN, Napoli (Italy); Mukha, I. [Gesellschaft fuer Schwerionenforschung, Damrstadt (Germany); Kurchatov Inst., Moscow (Russian Federation); Rykaczewski, K. [Oak Ridge National Laboratory, Oak Ridge (United States); Schmidt, K. [Continental Teves AG and Co., Frankfurt am Main (Germany); Schwengner, R. [Institut fuer Kern- und Hadronenphysik, Forschungszentrum Rossendorf, Dresden (Germany)


    The {beta} decay of the very neutron-deficient isotope {sup 101}Sn was studied at the GSI on-line mass separator using silicon detectors for recording charged particles and germanium detectors for {gamma}-ray spectroscopy. Based on the {beta}-delayed proton data the production cross-section of {sup 101}Sn in the {sup 50}Cr+{sup 58}Ni fusion-evaporation reaction was determined to be about 60nb. The half-life of {sup 101}Sn was measured to be 1.9(3)s. For the first time {beta}-delayed {gamma}-rays of {sup 101}Sn were tentatively identified, yielding weak evidence for a cascade of 352 and 1065keV transitions in {sup 101}In. The results for the {sup 101}Sn decay as well as those from previous work on the {sup 103}Sn decay are discussed by comparing them to predictions obtained from shell model calculations employing a new interaction in the {sup 88}Sr to {sup 132}Sn model space. (orig.)

  13. Modern Spectroscopy (United States)

    Barrow, Gordon M.


    Presents the basic ideas of modern spectroscopy. Both the angular momenta and wave-nature approaches to the determination of energy level patterns for atomic and molecular systems are discussed. The interpretation of spectra, based on atomic and molecular models, is considered. (LC)

  14. Bioimpedance Spectroscopy

    DEFF Research Database (Denmark)

    Klösgen, Beate; Rümenapp, Christine; Gleich, Bernhard


    causes relaxation processes with characteristic contributions to the frequency-dependent complex dielectric constant. These dipolar relaxations were initially described by Debye (Polare Molekeln 1929). They are the basis of impedance spectroscopy (K’Owino and Sadik Electroanalysis 17(23):2101–2113, 2005...

  15. Differential regulation of chemoattractant-stimulated beta 2, beta 3, and beta 7 integrin activity. (United States)

    Sadhu, C; Masinovsky, B; Staunton, D E


    Leukocyte adhesion to endothelium and extravasation are dynamic processes that require activation of integrins. Chemoattractants such as IL-8 and FMLP are potent activators of leukocyte integrins. To compare the chemoattractant-stimulated activation of three integrins, alpha 4 beta 7, alpha L beta 2, and alpha V beta 3, in the same cellular context, we expressed an IL-8 receptor (IL-8RA) and FMLP receptor (FPR) in the lymphoid cell line JY. Chemoattractants induced a rapid increase in alpha L beta 2- and alpha V beta 3-dependent JY adhesion within 5 min, and it was sustained for 30 min. In contrast, stimulation of alpha 4 beta 7-dependent adhesion was transient, returning to basal levels by 30 min. The activation profiles of the integrins were similar regardless of whether IL-8 or FMLP was used for induction. We also demonstrate that alpha 4 beta 7-dependent adhesion was uniquely responsive to the F actin-disrupting agent cytochalasin D and the protein kinase C (PKC) inhibitor chelerythrin. While alpha V beta 3- and alpha L beta 2-mediated cell adhesion was significantly reduced by cytochalasin D, alpha 4 beta 7-mediated adhesion was enhanced. Chelerythrin inhibited both the IL-8 and PMA activation of alpha L beta 2 and alpha V beta 3. In contrast, inducible alpha 4 beta 7 activity was unaffected, and basal activity was increased. These findings demonstrate that the mechanism of alpha 4 beta 7 regulation by chemoattractants is different from that of alpha L beta 2 and alpha V beta 3 and that it appears to involve distinct cytoskeletal and PKC dependencies. In addition, PKC activity may be a positive or negative regulator of integrin-dependent adhesion.

  16. A beta2-microglobulin cleavage variant fibrillates at near-physiological pH

    DEFF Research Database (Denmark)

    Corlin, Dorthe B; Johnsen, Christina K; Nissen, Mogens H


    Beta2-microglobulin (beta2m) deposits as amyloid in dialysis-related amyloidosis (DRA), predominantly in joints. The molecular mechanisms underlying the amyloidogenicity of beta2m are still largely unknown. In vitro, acidic conditions, pH ... several days. Here, we show that amyloid fibrils are generated in less than an hour when a cleavage variant of beta2m--found in the circulation of many dialysis patients--is exposed to pH levels (pH 6.6) occurring in joints during inflammation. Aggregation and fibrillation, including seeding effects...... with intact, native beta2m were studied by Thioflavin T fluorescence spectroscopy, turbidimetry, capillary electrophoresis, and electron microscopy. We conclude that a biologically relevant variant of beta2m is amyloidogenic at slightly acidic pH. Also, only a very small amount of preformed fibrils...

  17. Identification of a Novel Parallel beta-Strand Conformation within Molecular Monolayer of Amyloid Peptide

    DEFF Research Database (Denmark)

    Liu, Lei; Li, Qiang; Zhang, Shuai;


    technique with force controlled in pico-Newton range, combining with molecular dynamic simulation. The identified parallel beta-strand-like structure of molecular monolayer is distinct from the antiparallel beta-strand structure of A beta(33-42) amyloid fibril. This finding enriches the molecular structures....... In this work, the early A beta(33-42) aggregates forming the molecular monolayer at hydrophobic interface are investigated. The molecular monolayer of amyloid peptide A beta(33-42) consisting of novel parallel beta-strand-like structure is further revealed by means of a quantitative nanomechanical spectroscopy......The differentiation of protein properties and biological functions arises from the variation in the primary and secondary structure. Specifically, in abnormal assemblies of protein, such as amyloid peptide, the secondary structure is closely correlated with the stable ensemble and the cytotoxicity...

  18. Beta-thalassemia

    Directory of Open Access Journals (Sweden)

    Origa Raffaella


    Full Text Available Abstract Beta-thalassemias are a group of hereditary blood disorders characterized by anomalies in the synthesis of the beta chains of hemoglobin resulting in variable phenotypes ranging from severe anemia to clinically asymptomatic individuals. The total annual incidence of symptomatic individuals is estimated at 1 in 100,000 throughout the world and 1 in 10,000 people in the European Union. Three main forms have been described: thalassemia major, thalassemia intermedia and thalassemia minor. Individuals with thalassemia major usually present within the first two years of life with severe anemia, requiring regular red blood cell (RBC transfusions. Findings in untreated or poorly transfused individuals with thalassemia major, as seen in some developing countries, are growth retardation, pallor, jaundice, poor musculature, hepatosplenomegaly, leg ulcers, development of masses from extramedullary hematopoiesis, and skeletal changes that result from expansion of the bone marrow. Regular transfusion therapy leads to iron overload-related complications including endocrine complication (growth retardation, failure of sexual maturation, diabetes mellitus, and insufficiency of the parathyroid, thyroid, pituitary, and less commonly, adrenal glands, dilated myocardiopathy, liver fibrosis and cirrhosis. Patients with thalassemia intermedia present later in life with moderate anemia and do not require regular transfusions. Main clinical features in these patients are hypertrophy of erythroid marrow with medullary and extramedullary hematopoiesis and its complications (osteoporosis, masses of erythropoietic tissue that primarily affect the spleen, liver, lymph nodes, chest and spine, and bone deformities and typical facial changes, gallstones, painful leg ulcers and increased predisposition to thrombosis. Thalassemia minor is clinically asymptomatic but some subjects may have moderate anemia. Beta-thalassemias are caused by point mutations or, more rarely

  19. Beta-thalassemia. (United States)

    Galanello, Renzo; Origa, Raffaella


    Beta-thalassemias are a group of hereditary blood disorders characterized by anomalies in the synthesis of the beta chains of hemoglobin resulting in variable phenotypes ranging from severe anemia to clinically asymptomatic individuals. The total annual incidence of symptomatic individuals is estimated at 1 in 100,000 throughout the world and 1 in 10,000 people in the European Union. Three main forms have been described: thalassemia major, thalassemia intermedia and thalassemia minor. Individuals with thalassemia major usually present within the first two years of life with severe anemia, requiring regular red blood cell (RBC) transfusions. Findings in untreated or poorly transfused individuals with thalassemia major, as seen in some developing countries, are growth retardation, pallor, jaundice, poor musculature, hepatosplenomegaly, leg ulcers, development of masses from extramedullary hematopoiesis, and skeletal changes that result from expansion of the bone marrow. Regular transfusion therapy leads to iron overload-related complications including endocrine complication (growth retardation, failure of sexual maturation, diabetes mellitus, and insufficiency of the parathyroid, thyroid, pituitary, and less commonly, adrenal glands), dilated myocardiopathy, liver fibrosis and cirrhosis). Patients with thalassemia intermedia present later in life with moderate anemia and do not require regular transfusions. Main clinical features in these patients are hypertrophy of erythroid marrow with medullary and extramedullary hematopoiesis and its complications (osteoporosis, masses of erythropoietic tissue that primarily affect the spleen, liver, lymph nodes, chest and spine, and bone deformities and typical facial changes), gallstones, painful leg ulcers and increased predisposition to thrombosis. Thalassemia minor is clinically asymptomatic but some subjects may have moderate anemia. Beta-thalassemias are caused by point mutations or, more rarely, deletions in the beta

  20. Beta and muon decays

    Energy Technology Data Exchange (ETDEWEB)

    Galindo, A.; Pascual, P.


    These notes represent a series of lectures delivered by the authors in the Junta de Energia Nuclear, during the Spring term of 1965. They were devoted to graduate students interested in the Theory of Elementary Particles. Special emphasis was focussed into the computational problems. Chapter I is a review of basic principles (Dirac equation, transition probabilities, final state interactions.) which will be needed later. In Chapter II the four-fermion punctual Interaction is discussed, Chapter III is devoted to the study of beta-decay; the main emphasis is given to the deduction of the formulae corresponding to electron-antineutrino correlation, electron energy spectrum, lifetimes, asymmetry of electrons emitted from polarized nuclei, electron and neutrino polarization and time reversal invariance in beta decay. In Chapter IV we deal with the decay of polarized muons with radiative corrections. Chapter V is devoted to an introduction to C.V.C. theory. (Author)

  1. Realized Beta GARCH

    DEFF Research Database (Denmark)

    Hansen, Peter Reinhard; Lunde, Asger; Voev, Valeri Radkov


    We introduce a multivariate generalized autoregressive conditional heteroskedasticity (GARCH) model that incorporates realized measures of variances and covariances. Realized measures extract information about the current levels of volatilities and correlations from high-frequency data, which...... is particularly useful for modeling financial returns during periods of rapid changes in the underlying covariance structure. When applied to market returns in conjunction with returns on an individual asset, the model yields a dynamic model specification of the conditional regression coefficient that is known...... as the beta. We apply the model to a large set of assets and find the conditional betas to be far more variable than usually found with rolling-window regressions based exclusively on daily returns. In the empirical part of the paper, we examine the cross-sectional as well as the time variation...

  2. Coroutine Sequencing in BETA

    DEFF Research Database (Denmark)

    Kristensen, Bent Bruun; Madsen, Ole Lehrmann; Møller-Pedersen, Birger;

    In object-oriented programming, a program execution is viewed as a physical model of some real or imaginary part of the world. A language supporting object-oriented programming must therefore contain comprehensive facilities for modeling phenomena and concepts form the application domain. Many ap...... applications in the real world consist of objects carrying out sequential processes. Coroutines may be used for modeling objects that alternate between a number of sequential processes. The authors describe coroutines in BETA...

  3. Magic Baseline Beta Beam

    CERN Document Server

    Agarwalla, Sanjib Kumar; Raychaudhuri, Amitava


    We study the physics reach of an experiment where neutrinos produced in a beta-beam facility at CERN are observed in a large magnetized iron calorimeter (ICAL) at the India-based Neutrino Observatory (INO). The CERN-INO distance is close to the so-called "magic" baseline which helps evade some of the parameter degeneracies and allows for a better measurement of the neutrino mass hierarchy and $\\theta_{13}$.

  4. Beta cell adaptation in pregnancy

    DEFF Research Database (Denmark)

    Nielsen, Jens Høiriis


    Pregnancy is associated with a compensatory increase in beta cell mass. It is well established that somatolactogenic hormones contribute to the expansion both indirectly by their insulin antagonistic effects and directly by their mitogenic effects on the beta cells via receptors for prolactin...... and growth hormone expressed in rodent beta cells. However, the beta cell expansion in human pregnancy seems to occur by neogenesis of beta cells from putative progenitor cells rather than by proliferation of existing beta cells. Claes Hellerström has pioneered the research on beta cell growth for decades......, but the mechanisms involved are still not clarified. In this review the information obtained in previous studies is recapitulated together with some of the current attempts to resolve the controversy in the field: identification of the putative progenitor cells, identification of the factors involved...

  5. Regulation of beta cell replication

    DEFF Research Database (Denmark)

    Lee, Ying C; Nielsen, Jens Høiriis


    Beta cell mass, at any given time, is governed by cell differentiation, neogenesis, increased or decreased cell size (cell hypertrophy or atrophy), cell death (apoptosis), and beta cell proliferation. Nutrients, hormones and growth factors coupled with their signalling intermediates have been...... suggested to play a role in beta cell mass regulation. In addition, genetic mouse model studies have indicated that cyclins and cyclin-dependent kinases that determine cell cycle progression are involved in beta cell replication, and more recently, menin in association with cyclin-dependent kinase...... inhibitors has been demonstrated to be important in beta cell growth. In this review, we consider and highlight some aspects of cell cycle regulation in relation to beta cell replication. The role of cell cycle regulation in beta cell replication is mostly from studies in rodent models, but whether...

  6. LHCb: $2\\beta_s$ measurement at LHCb

    CERN Multimedia

    Conti, G


    A measurement of $2\\beta_s$, the phase of the $B_s-\\bar{B_s}$ oscillation amplitude with respect to that of the ${\\rm b} \\rightarrow {\\rm c^{+}}{\\rm W^{-}}$ tree decay amplitude, is one of the key goals of the LHCb experiment with first data. In the Standard Model (SM), $2\\beta_s$ is predicted to be $0.0360^{+0.0020}_{-0.0016} \\rm rad$. The current constraints from the Tevatron are: $2\\beta_{s}\\in[0.32 ; 2.82]$ at 68$\\%$CL from the CDF experiment and $2\\beta_{s}=0.57^{+0.24}_{-0.30}$ from the D$\\oslash$ experiment. Although the statistical uncertainties are large, these results hint at the possible contribution of New Physics in the $B_s-\\bar{B_s}$ box diagram. After one year of data taking at LHCb at an average luminosity of $\\mathcal{L}\\sim2\\cdot10^{32}\\rm cm^{-2} \\rm s^{-1}$ (integrated luminosity $\\mathcal{L}_{\\rm int}\\sim 2 \\rm fb^{-1}$), the expected statistical uncertainty on the measurement is $\\sigma(2\\beta_s)\\simeq 0.03$. This uncertainty is similar to the $2\\beta_s$ value predicted by the SM.

  7. Astronomical Spectroscopy

    CERN Document Server

    Massey, Philip


    Spectroscopy is one of the most important tools that an astronomer has for studying the universe. This chapter begins by discussing the basics, including the different types of optical spectrographs, with extension to the ultraviolet and the near-infrared. Emphasis is given to the fundamentals of how spectrographs are used, and the trade-offs involved in designing an observational experiment. It then covers observing and reduction techniques, noting that some of the standard practices of flat-fielding often actually degrade the quality of the data rather than improve it. Although the focus is on point sources, spatially resolved spectroscopy of extended sources is also briefly discussed. Discussion of differential extinction, the impact of crowding, multi-object techniques, optimal extractions, flat-fielding considerations, and determining radial velocities and velocity dispersions provide the spectroscopist with the fundamentals needed to obtain the best data. Finally the chapter combines the previous materi...

  8. Grain Spectroscopy (United States)

    Allamandola, L. J.


    Our fundamental knowledge of interstellar grain composition has grown substantially during the past two decades thanks to significant advances in two areas: astronomical infrared spectroscopy and laboratory astrophysics. The opening of the mid-infrared, the spectral range from 4000-400 cm(sup -1) (2.5-25 microns), to spectroscopic study has been critical to this progress because spectroscopy in this region reveals more about a materials molecular composition and structure than any other physical property. Infrared spectra which are diagnostic of interstellar grain composition fall into two categories: absorption spectra of the dense and diffuse interstellar media, and emission spectra from UV-Vis rich dusty regions. The former will be presented in some detail, with the latter only very briefly mentioned. This paper summarized what we have learned from these spectra and presents 'doorway' references into the literature. Detailed reviews of many aspects of interstellar dust are given.

  9. Drug carrier systems based on water-soluble cationic beta-cyclodextrin polymers. (United States)

    Li, Jianshu; Xiao, Huining; Li, Jiehua; Zhong, YinPing


    This study was designed to synthesize, characterize and investigate the drug inclusion property of a series of novel cationic beta-cyclodextrin polymers (CPbetaCDs). Proposed water-soluble polymers were synthesized from beta-cyclodextrin (beta-CD), epichlorohydrin (EP) and choline chloride (CC) through a one-step polymerization procedure by varying molar ratio of EP and CC to beta-CD. Physicochemical properties of the polymers were characterized with colloidal titration, nuclear magnetic resonance spectroscopy (NMR), gel permeation chromatography (GPC) and aqueous solubility determination. The formation of naproxen/CPbetaCDs inclusion complexes was confirmed by NMR and fourier transform infrared spectroscopy (FT-IR). Cationic beta-CD polymers showed better hemolytic activities than parent beta-CD and neutral beta-CD polymer in hemolysis test. The morphological study of erythrocytes revealed a cell membrane invagination induced by the cationic groups. The effects of molecular weight and charge density of the polymers on their inclusion and release performance of naproxen were also investigated through phase-solubility and dissolution studies. It was found that the cationic beta-CD polymers with high molecular weight or low charge density exhibited better drug inclusion and dissolution abilities.

  10. Precision Study of the $\\beta$-decay of $^{62}$Ga

    CERN Multimedia


    It is proposed to perform a precision study of the $\\beta$-decay of $\\,^{62}$Ga taking advantage of recent developments of the ISOLDE Laser Ion Source. The goal is to eventually extend the high-precision knowledge of superallowed $\\beta$-decays beyond the nine decays that presently are used for extracting the V$_{ud}$ quark mixing matrix element of the CKM matrix. The scientific motivations are the current deviation of more than 2$\\sigma$ of the unitary condition of this matrix, which could be an indication of non-standard-model physics, and a test of the theoretical corrections applied to the experimental data. The experiment will utilise the Total Absorption $\\gamma$-ray (TAG) spectrometer in order to determine weak branchings to excited states in $^{62}$Zn and the ISOLDE spectroscopy station to perform half-life measurements and detailed spectroscopy of this nucleus.

  11. Precision study of the $\\beta$-decay of $^{74}$Rb

    CERN Multimedia

    Van Duppen, P L E; Lunney, D


    We are proposing a high-resolution study of the $\\beta$-decay of $^{74}$Rb in order to extrapolate our precision knowledge of the superallowed $\\beta$-decays from the sd and fp shells towards the medium-heavy Z=N nuclei. The primary goal is to provide new data for testing the CVC hypothesis and the unitarity condition of the CKM matrix of the Standard Model. The presented programme would involve the careful measurements of the decay properties of $^{74}$Rb including the branching ratios to the excited states as well as the precise determination of the decay energy of $^{74}$Rb. The experimental methods readily available at ISOLDE include high-transmission conversion electron spectroscopy, $\\gamma$-ray spectroscopy as well as the measurements of the masses of $^{74}$Rb and $^{74}$Kr using two complementary techniques, ISOLTRAP and MISTRAL. The experiment would rely on a high-quality $^{74}$Rb beam available at ISOLDE with adequate intensity.

  12. Beta Decay of 101Sn

    Energy Technology Data Exchange (ETDEWEB)

    Kavatsyuk, O. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Mazzocchi, C. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Janas, Z. [University of Warsaw; Banu, A. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Batist, L. [St. Petersburg Nuclear Physics Institute; Becker, F. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Blazhev, A. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Bruchle, W. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Doring, J. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Faestermann, T. [Technische Universitat Munchen; Gorska, M. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Grawe, H. [GSI-Hemholtzzentrum fur Schwerionenforschung, Darmstadt, Germany; Jungclaus, A. [Instituto Estructura de la Materia, Madrid; Karny, M. [University of Warsaw; Kavatsyuk, M. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Klepper, O. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Kirchner, R. [Gesellschaft fur Schwerionenforschung (GSI), Germany; La Commara, M. [Universita Federico II and INFN Napoli; Miernik, K. [University of Warsaw; Mukha, I. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Plettner, C. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Plochocki, A. [University of Warsaw; Roeckl, E. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Romoli, M. [Universita Federico II and INFN Napoli; Rykaczewski, Krzysztof Piotr [ORNL; Schadel, M. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Schmidt, K. [Continental Teves AG & Co., Frankfurt am Main, Germany; Schwengner, R. [Forschungszentrum Rossendorf, Dresden, Germany; Zylicz, J. [University of Warsaw


    The {beta} decay of the very neutron-deficient isotope 101Sn was studied at the GSI on-line mass separator using silicon detectors for recording charged particles and germanium detectors for {gamma}-ray spectroscopy. Based on the {beta}-delayed proton data the production cross-section of 101Sn in the 50Cr + 58Ni fusion-evaporation reaction was determined to be about 60nb. The half-life of 101Sn was measured to be 1.9(3)s. For the first time {beta}-delayed {gamma}-rays of 101Sn were tentatively identified, yielding weak evidence for a cascade of 352 and 1065keV transitions in 101In. The results for the 101Sn decay as well as those from previous work on the 103Sn decay are discussed by comparing them to predictions obtained from shell model calculations employing a new interaction in the 88Sr to 132Sn model space.

  13. Project 8 Phase II: Improved beta decay electrons reconstruction (United States)

    Guigue, Mathieu; Project 8 Collaboration


    The Project 8 collaboration aims to measure the absolute neutrino mass scale using a cyclotron radiation emission spectroscopy technique on the beta decays of tritium. The second phase of the project will measure a differential spectrum of tritium beta decays and extract the tritium endpoint value with an eV or sub-eV scale precision. Monoenergetic electrons emitted by gaseous 83mKr atoms can be used to determine the coefficient between the cyclotron frequency and the electron energy and to optimize the instrument configuration for the tritium measurement. We present the progress on the processing of the electron cyclotron radiation signal to reconstruct the beta decay spectrum of krypton and tritium.

  14. Laser spectroscopy

    CERN Document Server

    Demtröder, Wolfgang


    Keeping abreast of the latest techniques and applications, this new edition of the standard reference and graduate text on laser spectroscopy has been completely revised and expanded. While the general concept is unchanged, the new edition features a broad array of new material, e.g., frequency doubling in external cavities, reliable cw-parametric oscillators, tunable narrow-band UV sources, more sensitive detection techniques, tunable femtosecond and sub-femtosecond lasers (X-ray region and the attosecond range), control of atomic and molecular excitations, frequency combs able to synchronize independent femtosecond lasers, coherent matter waves, and still more applications in chemical analysis, medical diagnostics, and engineering.

  15. Beta section Beta: biogeographical patterns of variation and taxonomy.

    NARCIS (Netherlands)

    Letschert, J.P.W.


    In Chapter 1 an account is given of the historical subdivision of the genus Beta and its sections, and the relations of the sections are discussed. Emphasis is given to the taxonomic treatment of wild section Beta by various authors. The Linnaean names B. vulgaris L. and B. maritima L. are lectotypi

  16. Cyclic modular beta-sheets. (United States)

    Woods, R Jeremy; Brower, Justin O; Castellanos, Elena; Hashemzadeh, Mehrnoosh; Khakshoor, Omid; Russu, Wade A; Nowick, James S


    The development of peptide beta-hairpins is problematic, because folding depends on the amino acid sequence and changes to the sequence can significantly decrease folding. Robust beta-hairpins that can tolerate such changes are attractive tools for studying interactions involving protein beta-sheets and developing inhibitors of these interactions. This paper introduces a new class of peptide models of protein beta-sheets that addresses the problem of separating folding from the sequence. These model beta-sheets are macrocyclic peptides that fold in water to present a pentapeptide beta-strand along one edge; the other edge contains the tripeptide beta-strand mimic Hao [JACS 2000, 122, 7654] and two additional amino acids. The pentapeptide and Hao-containing peptide strands are connected by two delta-linked ornithine (deltaOrn) turns [JACS 2003, 125, 876]. Each deltaOrn turn contains a free alpha-amino group that permits the linking of individual modules to form divalent beta-sheets. These "cyclic modular beta-sheets" are synthesized by standard solid-phase peptide synthesis of a linear precursor followed by solution-phase cyclization. Eight cyclic modular beta-sheets 1a-1h containing sequences based on beta-amyloid and macrophage inflammatory protein 2 were synthesized and characterized by 1H NMR. Linked cyclic modular beta-sheet 2, which contains two modules of 1b, was also synthesized and characterized. 1H NMR studies show downfield alpha-proton chemical shifts, deltaOrn delta-proton magnetic anisotropy, and NOE cross-peaks that establish all compounds but 1c and 1g to be moderately or well folded into a conformation that resembles a beta-sheet. Pulsed-field gradient NMR diffusion experiments show little or no self-association at low (

  17. Physico-chemical characterization of benzocaine-beta-cyclodextrin inclusion complexes. (United States)

    Pinto, Luciana M A; Fraceto, Leonardo Fernandes; Santana, Maria Helena A; Pertinhez, Thelma A; Junior, Sérgio Oyama; de Paula, Eneida


    Local anesthetics are able to induce pain relief by binding to the sodium channel of excitable membranes, blocking the influx of sodium ions and the propagation of the nervous impulse. Benzocaine (BZC) is a local anesthetic whose low water-solubility limits its application to topical formulations. The present work focuses on the characterization of inclusion complexes of BZC in beta-cyclodextrin (beta-CD). Differential scanning calorimetry and electron microscopy gave evidences of the formation and the morphology of the complex. Fluorescence spectroscopy showed a BZC/beta-CD 1:1 stoichiometry. Phase-solubility diagrams allowed the determination of the association constants between BZC and beta-CD (549 M(-1)) and revealed that a three-fold increase in BZC solubility can be reached upon complexation with beta-CD. The details of BZC/beta-CD molecular interaction were analyzed by 1H 2D NMR allowing the proposition of an inclusion model for BZC into beta-CD where the aromatic ring of the anesthetic is located near the head of the beta-CD cavity. Moreover, in preliminary toxicity studies, the complex seems to be less toxic than BZC alone, since it induced a decrease in the in vitro oxidation of human hemoglobin. These results suggest that the BZC/beta-CD complex represents an effective novel formulation to enhance BZC solubility in water, turning it promising for use outside its traditional application, i.e., in infiltrative anesthesia.

  18. Integration of BETA with Eclipse

    DEFF Research Database (Denmark)

    Andersen, Peter; Madsen, Ole Lehrmann; Enevoldsen, Mads Brøgger


    This paper presents language interoperability issues appearing in order to implement support for the BETA language in the Java-based Eclipse integrated development environment. One of the challenges is to implement plug-ins in BETA and be able to load them in Eclipse. In order to do this, some form...

  19. Measurements of sin 2 $\\beta$

    CERN Document Server

    Tricomi, A


    A review of the most recent measurements of the CP violating parameter sin 2 beta from LEP and CDF is reported. These yield an average value of sin 2 beta =0.91+or-0.35, giving a confidence level that CP violation in the B system has been observed of almost 99%. (10 refs).

  20. Beta decay of Cu-56

    NARCIS (Netherlands)

    Borcea, R; Aysto, J; Caurier, E; Dendooven, P; Doring, J; Gierlik, M; Gorska, M; Grawe, H; Hellstrom, M; Janas, Z; Jokinen, A; Karny, M; Kirchner, R; La Commara, M; Langanke, K; Martinez-Pinedo, G; Mayet, P; Nieminen, A; Nowacki, F; Penttila, H; Plochocki, A; Rejmund, M; Roeckl, E; Schlegel, C; Schmidt, K; Schwengner, R; Sawicka, M


    The proton-rich isotope Cu-56 was produced at the GSI On-Line Mass Separator by means of the Si-28(S-32, p3n) fusion-evaporation reaction. Its beta -decay properties were studied by detecting beta -delayed gamma rays and protons. A half-Life of 93 +/- 3 ms was determined for Cu-56. Compared to the p

  1. Beta Function and Anomalous Dimensions

    DEFF Research Database (Denmark)

    Pica, Claudio; Sannino, Francesco


    We demonstrate that it is possible to determine the coefficients of an all-order beta function linear in the anomalous dimensions using as data the two-loop coefficients together with the first one of the anomalous dimensions which are universal. The beta function allows to determine the anomalou...

  2. Design and production of sintered {beta}-tricalcium phosphate 3D scaffolds for bone tissue regeneration

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Carlos F.L. [CICS-UBI - Centro de Investigacao em Ciencias da Saude, Universidade da Beira Interior, Covilha (Portugal); Silva, Abilio P. [Centro de Ciencia e Tecnologia Aeroespaciais, Universidade da Beira Interior, Covilha (Portugal); Lopes, Luis [CICS-UBI - Centro de Investigacao em Ciencias da Saude, Universidade da Beira Interior, Covilha (Portugal); Pires, Ines [Instituto de Engenharia Mecanica - Lisboa (IDMEC Lisboa/IST/UTL), Avenida Rovisco Pais, 1049-001 Lisboa (Portugal); Correia, Ilidio J., E-mail: [CICS-UBI - Centro de Investigacao em Ciencias da Saude, Universidade da Beira Interior, Covilha (Portugal)


    The characteristics of sintered {beta}-tricalcium phosphate ({beta}-TCP) scaffolds produced by 3D printing were studied by means of X-ray diffraction, Scanning Electron Microscopy, Fourier transform infrared spectroscopy, uniaxial compression tests and cytotoxicity tests, using human osteoblast cells. The results reported include details of the {beta}-TCP scaffolds' porosity, density, phase stability, mechanical behavior and cytotoxic profile. Collectively, these properties are fundamental for the future application of these scaffolds as bone substitutes for individualized therapy. Highlights: Black-Right-Pointing-Pointer {beta}-Tricalcium phosphate ({beta}-TCP) 3D scaffolds were produced by rapid prototyping. Black-Right-Pointing-Pointer Scaffold properties were assessed by SEM, FTIR, XRD and by mechanical tests. Black-Right-Pointing-Pointer The cytotoxic profile of the scaffolds was characterized by in vitro assays. Black-Right-Pointing-Pointer Scaffolds have good properties for its application as bone substitutes for individualized therapy.

  3. Thermodynamics of complexes between nucleobase-modified {beta}-cyclodextrins and bile salts

    Energy Technology Data Exchange (ETDEWEB)

    Liu Yu [Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, 300071 Tianjin (China)], E-mail:; Zhang Qian; Guo Dongsheng; Zhuang Ruijie; Wang Lihua [Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, 300071 Tianjin (China)


    The binding of three nucleobase-modified {beta}-CDs, (i.e., mono(6-ade-6-deoxy)-{beta}-CD 2, mono(6-thy-6-deoxy)-{beta}-CD 3, and mono(6-ura-6-deoxy)-{beta}-CD 4) with four bile salts (deoxycholate, DCA; cholate, CA; glycocholate, GCA; and taurocholate, TCA) were investigated by means of circular dichroism, 2D NMR spectroscopy and calorimetric titration. The results show the binding of host 2 with bile salts is weaker and different from hosts 3 and 4. Enthalpy changes between hosts 2-4 and bile salts are much more favorable than those of native {beta}-CD 1, whereas the entropy changes are unfavorable.

  4. Compatibility of the antitumoral beta-lapachone with different solid dosage forms excipients. (United States)

    Cunha-Filho, Marcílio S S; Martínez-Pacheco, Ramón; Landín, Mariana


    The objective of the present study was to evaluate the compatibility of the beta-lapachone (betaLAP), an antitumoral drug in clinical phase, with pharmaceutical excipients of common use including diluents, binders, disintegrants, lubricants and solubilising agents. Differential scanning calorimetry (DSC) was used for a first screening to find small variations in peak temperatures and/or their associated enthalpy for six drug/excipient combinations (magnesium stearate, sodium estearyl fumarate, dicalcium phosphate dihydrate, mannitol, randomized methyl-beta-cyclodextrin and hydroxypropyl-beta-cyclodextrin), which indicate some degree of interaction. Additional studies using Fourier transformed infrared spectroscopy (FTIR), optical microscopy (OM) and heating-cooling DSC (HC-DSC) confirmed the incompatibility of betaLAP with magnesium stearate and dicalcium phosphate dihydrate. Those excipients should be avoided in the development of solid dosage forms.

  5. RAVEN Beta Release

    Energy Technology Data Exchange (ETDEWEB)

    Rabiti, Cristian [Idaho National Lab. (INL), Idaho Falls, ID (United States); Alfonsi, Andrea [Idaho National Lab. (INL), Idaho Falls, ID (United States); Cogliati, Joshua Joseph [Idaho National Lab. (INL), Idaho Falls, ID (United States); Mandelli, Diego [Idaho National Lab. (INL), Idaho Falls, ID (United States); Kinoshita, Robert Arthur [Idaho National Lab. (INL), Idaho Falls, ID (United States); Wang, Congjian [Idaho National Lab. (INL), Idaho Falls, ID (United States); Maljovec, Daniel Patrick [Idaho National Lab. (INL), Idaho Falls, ID (United States); Talbot, Paul William [Idaho National Lab. (INL), Idaho Falls, ID (United States)


    This documents the release of the Risk Analysis Virtual Environment (RAVEN) code. A description of the RAVEN code is provided, and discussion of the release process for the M2LW-16IN0704045 milestone. The RAVEN code is a generic software framework to perform parametric and probabilistic analysis based on the response of complex system codes. RAVEN is capable of investigating the system response as well as the input space using Monte Carlo, Grid, or Latin Hyper Cube sampling schemes, but its strength is focused toward system feature discovery, such as limit surfaces, separating regions of the input space leading to system failure, using dynamic supervised learning techniques. RAVEN has now increased in maturity enough for the Beta 1.0 release.

  6. Fluorination of (E)-{beta}-(fluoromethylene)-m-tyrosine: Regioslective synthesis of 4-fluoro-(E)-{beta}-(fluoromethylene)-m-tyrosine

    Energy Technology Data Exchange (ETDEWEB)

    Lacan, G.; Satyamurthy, N.; Barrio, J.R. [UCLA School of Medicine and Lab. of Structural Biology and Molecular Medicine, Los Angeles, CA (United States)


    Fluorination of (R)- and (S)-(E)-{beta}-(fluoromethylene)-m-tyrosine (1) by acetyl hypofluorite yielded a mixture of the corresponding 2-fluoro- (2a), 6-fluoro- (2b), 4-fluoro- (2c), and 2,6-difluoro- (2d) derivatives along with a pair of diastereomeric products of addition across the vinylic double bond. A regioselective synthesis of 4-fluoro-(E)-{beta}-(fluoromethylene)-m-tyrosine has also been developed based on a fluorodestannylation reaction with elemental fluorine followed by acid hydrolysis. This reaction sequence yielded minor byproducts, namely, 4-fluoro-(Z)-{beta}(fluoromethylene)-m-tyrosine (11), 4,6-difluoro- and 2,4-difluoro-(E)-{beta}-(fluoromethylene)-m-tyrosines (12a and 12b). All these products were completely separated by semipreparative HPLC and fully characterized by NMR and mass spectroscopy. Single crystal X-ray crystallographic analyses of 2-fluoro-, 4-fluoro-, and 6-fluoro-(E)-{beta}-(fluoromethylene)-m-tyrosines unequivocally established the structures of these amino acids. 1 fig., 3 tabs.

  7. Inclusion phenomena of clove oil with alpha-, beta-, gamma- and heptakis (2,6-di-O-methyl)-beta-cyclodextrin. (United States)

    Song, L X; Xu, P; Wang, H M; Yang, Y


    Inclusion interactions of alpha-, beta-, gamma- and heptakis (2,6-di-O-methyl)-beta-cyclodextrin (DMbeta-CD) as hosts with clove oil (an impure eugenol, I-Eug) as guest in aqueous solution were investigated by fluorescence emission spectra. The binding constants of different hosts to I-Eug in aqueous solution decreased in the order: gamma- > beta- > DMbeta- > alpha-CD. Two solid supramolecular inclusion complexes, I-Eug-beta-CD and I-Eug-gamma-CD, were prepared and characterised by nuclear magnetic resonance, powder X-ray diffraction, infrared spectroscopy, and thermogravimetric analysis. All the results proved the formation of I-Eug-CD. The inclusion differences between I-Eug and pure eugenol were discussed. The relative contents of the main component eugenol (Eug), second component (eugenol acetate, Eua) and others in I-Eug were found to be fairly different before and after being included by beta-CD, according to the data obtained from high performance liquid chromatography. This could be a practical method to extract the effective components (Eug and Eua) from I-Eug.

  8. [Serum beta 2 microglobulin (beta 2M) following renal transplantation]. (United States)

    Pacheco-Silva, A; Nishida, S K; Silva, M S; Ramos, O L; Azjen, H; Pereira, A B


    Although there was an important improvement in graft and patient survival the last 10 years, graft rejection continues to be a major barrier to the success of renal transplantation. Identification of a laboratory test that could help to diagnose graft rejection would facilitate the management of renal transplanted patients. PURPOSE--To evaluate the utility of monitoring serum beta 2M in recently transplanted patients. METHODS--We daily determined serum beta 2M levels in 20 receptors of renal grafts (10 from living related and 10 from cadaveric donors) and compared them to their clinical and laboratory evolution. RESULTS--Eight patients who presented immediate good renal function following grafting and did not have rejection had a mean serum beta 2M of 3.7 mg/L on the 4th day post transplant. The sensitivity of the test for the diagnosis of acute rejection was 87.5%, but the specificity was only 46%. Patients who presented acute tubular necrosis (ATN) without rejection had a progressive decrease in their serum levels of beta 2M, while their serum creatinine changed as they were dialyzed. In contrast, patients with ATN and concomitance of acute rejection or CSA nephrotoxicity presented elevated beta 2M and creatinine serum levels. CONCLUSION--Daily monitoring of serum beta 2M does not improve the ability to diagnose acute rejection in patients with good renal function. However, serum beta 2M levels seemed to be useful in diagnosing acute rejection or CSA nephrotoxicity in patients with ATN.

  9. Evidence for novel beta-sheet structures in Iowa mutant beta-amyloid fibrils. (United States)

    Tycko, Robert; Sciarretta, Kimberly L; Orgel, Joseph P R O; Meredith, Stephen C


    Asp23-to-Asn mutation within the coding sequence of beta-amyloid, called the Iowa mutation, is associated with early onset, familial Alzheimer's disease and cerebral amyloid angiopathy, in which patients develop neuritic plaques and massive vascular deposition predominantly of the mutant peptide. We examined the mutant peptide, D23N-Abeta40, by electron microscopy, X-ray diffraction, and solid-state NMR spectroscopy. D23N-Abeta40 forms fibrils considerably faster than the wild-type peptide (k = 3.77 x 10(-3) min(-1) and 1.07 x 10(-4) min(-1) for D23N-Abeta40 and the wild-type peptide WT-Abeta40, respectively) and without a lag phase. Electron microscopy shows that D23N-Abeta40 forms fibrils with multiple morphologies. X-ray fiber diffraction shows a cross-beta pattern, with a sharp reflection at 4.7 A and a broad reflection at 9.4 A, which is notably smaller than the value for WT-Abeta40 fibrils (10.4 A). Solid-state NMR measurements indicate molecular level polymorphism of the fibrils, with only a minority of D23N-Abeta40 fibrils containing the in-register, parallel beta-sheet structure commonly found in WT-Abeta40 fibrils and most other amyloid fibrils. Antiparallel beta-sheet structures in the majority of fibrils are indicated by measurements of intermolecular distances through (13)C-(13)C and (15)N-(13)C dipole-dipole couplings. An intriguing possibility exists that there is a relationship between the aberrant structure of D23N-Abeta40 fibrils and the unusual vasculotropic clinical picture in these patients.

  10. Effect of beta-cyclodextrin nanocavity confinement on the photophysics of robinetin. (United States)

    Banerjee, Anwesha; Basu, Kaushik; Sengupta, Pradeep K


    We have studied the confinement of robinetin, a therapeutically active plant flavonol, in cyclodextrin (CDx) nanocavities, using steady state and time resolved fluorescence spectroscopy. Enhanced tautomer emission (arising from excited state intramolecular proton transfer (ESIPT)) as well as dramatically blue shifted (approximately 10 nm in beta-CDx and approximately 33 nm in SHP beta-CDx) normal fluorescence observed upon addition of the beta-CDxs indicate that robinetin readily enters the doughnut-shaped hydrophobic cavity of beta-CDx where the chromone moiety is well shielded from external hydrogen bonding perturbations. Detailed analyses of the fluorescence data (emission profile, anisotropy, decay times) indicate that robinetin forms 1:1 inclusion complexes with both natural and chemically modified beta-cyclodextrins (beta-CDx and SHP beta-CDx) with affinity constant values K=195+/-17 M(-1) and 1055+/-48 M(-1) respectively, indicating the prospective utility of SHP beta-CDx in particular as an effective drug carrier. Unlike beta-CDxs, alpha-CDxs do not form inclusion complexes with robinetin. To further characterize the robinetin/beta-CDxs complexes, circular dichroism (CD) spectroscopic studies have been performed, which reveal that incorporation of robinetin molecules in the chiral environment of the beta-CDxs strongly affects the electronic transitions of robinetin leading to the occurrence of positive induced circular dichroism (ICD) bands in the near ultra-violet (UV) region. Molecular mechanics calculations show that the inclusion complex with the chromone ring inserted into the beta-CDx cavity is most favorable, in agreement with our spectroscopic data.

  11. Experiments on double beta decay

    Energy Technology Data Exchange (ETDEWEB)

    Busto, J. [Neuchatel Univ. (Switzerland). Inst. de Physique


    The Double Beta Decay, and especially ({beta}{beta}){sub 0{nu}} mode, is an excellent test of Standard Model as well as of neutrino physics. From experimental point of view, a very large number of different techniques are or have been used increasing the sensitivity of this experiments quite a lot (the factor of 10{sup 4} in the last 20 years). In future, in spite of several difficulties, the sensitivity would be increased further, keeping the interest of this very important process. (author) 4 figs., 5 tabs., 21 refs.

  12. Dosimetry of {beta} extensive sources; Dosimetria de fuentes {beta} extensas

    Energy Technology Data Exchange (ETDEWEB)

    Rojas C, E.L.; Lallena R, A.M. [Departamento de Fisica Moderna, Universidad de Granada, E-18071 Granada (Spain)


    In this work, we have been studied, making use of the Penelope Monte Carlo simulation code, the dosimetry of {beta} extensive sources in situations of spherical geometry including interfaces. These configurations are of interest in the treatment of the called cranealfaringyomes of some synovia leisure of knee and other problems of interest in medical physics. Therefore, its application can be extended toward problems of another areas with similar geometric situation and beta sources. (Author)

  13. Introduction to Spin Label Electron Paramagnetic Resonance Spectroscopy of Proteins (United States)

    Melanson, Michelle; Sood, Abha; Torok, Fanni; Torok, Marianna


    An undergraduate laboratory exercise is described to demonstrate the biochemical applications of electron paramagnetic resonance (EPR) spectroscopy. The beta93 cysteine residue of hemoglobin is labeled by the covalent binding of 3-maleimido-proxyl (5-MSL) and 2,2,5,5-tetramethyl-1-oxyl-3-methyl methanethiosulfonate (MTSL), respectively. The excess…

  14. Evolution of outer membrane beta-barrels from an ancestral beta beta hairpin. (United States)

    Remmert, M; Biegert, A; Linke, D; Lupas, A N; Söding, J


    Outer membrane beta-barrels (OMBBs) are the major class of outer membrane proteins from Gram-negative bacteria, mitochondria, and plastids. Their transmembrane domains consist of 8-24 beta-strands forming a closed, barrel-shaped beta-sheet around a central pore. Despite their obvious structural regularity, evidence for an origin by duplication or for a common ancestry has not been found. We use three complementary approaches to show that all OMBBs from Gram-negative bacteria evolved from a single, ancestral beta beta hairpin. First, we link almost all families of known single-chain bacterial OMBBs with each other through transitive profile searches. Second, we identify a clear repeat signature in the sequences of many OMBBs in which the repeating sequence unit coincides with the structural beta beta hairpin repeat. Third, we show that the observed sequence similarity between OMBB hairpins cannot be explained by structural or membrane constraints on their sequences. The third approach addresses a longstanding problem in protein evolution: how to distinguish between a very remotely homologous relationship and the opposing scenario of "sequence convergence." The origin of a diverse group of proteins from a single hairpin module supports the hypothesis that, around the time of transition from the RNA to the protein world, proteins arose by amplification and recombination of short peptide modules that had previously evolved as cofactors of RNAs.

  15. Beta-Thioxoketones

    DEFF Research Database (Denmark)

    Jørgensen, F. S.; Brown, R. S.; Carlsen, Lars;


    X-ray photoelectron spectroscopy has been used to record the 01, and Szp ionization spectra of thioacetylacetone, 2-acetylcyclohexanethione,2 -thioacetylcyclohexanone,th e S-methyl derivative of thioacetylacetone, and propyl 3-mercaptocrotonate in the gas phase. It is shown that both the enol...... and the enethiol tautomers of the /3-thioxoketones can be detected, and the enol/enethiol ratios for thioacetylacetone, 2-acetylcyclohexanethione, and 2-thioacetylcyclohexanone were determined to be 61:39, 30:70, and 80:20, respectively, based on the intensities of the oxygen ionizations. The conclusions derived...... from the sulfur region support the above, although they are less clear due to sulfur spin-orbit splitting. The enol/enethiol ratios obtained in the gas phase by XPS are compared with data from other methods, showing good agreement between results obtained in the gas phase and in solution. The binding...

  16. State-of-the-Art Beta Detection and Dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    David M. Hamby


    The research funded by this NEER grant establishes the framework for a detailed understanding of the challenges in beta dosimetry, especially in the presence of a mixed radiation field. The work also stimulated the thinking of the research group which will lead to new concepts in digital signal processing to allow collection of detection signals and real-time analysis such that simultaneous beta and gamma spectroscopy can take place. The work described herein (with detail in the many publications that came out of this research) was conducted in a manner that provided dissertation and thesis topics for three students, one of whom was completely funded by this grant. The overall benefit of the work came in the form of a dramatic shift in signal processing that is normally conducted in analog pulse shape analysis. Analog signal processing was shown not to be feasible for this type of work; digital signal processing was a must. This, in turn, led the research team to a new understanding of pulse analysis, one in which expands the state-of-the-art in simultaneous beta and gamma spectroscopy with a single detector.

  17. Identification of distinct physiochemical properties of toxic prefibrillar species formed by A{beta} peptide variants

    Energy Technology Data Exchange (ETDEWEB)

    Goeransson, Anna-Lena, E-mail: [Division of Molecular Biotechnology, Department of Physics, Chemistry and Biology, Linkoeping University (Sweden); Nilsson, K. Peter R., E-mail: [Division of Organic Chemistry, Department of Physics, Chemistry and Biology, Linkoeping University (Sweden); Kagedal, Katarina, E-mail: [Department of Clinical and Experimental Medicine, Linkoeping University (Sweden); Brorsson, Ann-Christin, E-mail: [Division of Molecular Biotechnology, Department of Physics, Chemistry and Biology, Linkoeping University (Sweden)


    Highlights: Black-Right-Pointing-Pointer Identification of toxic prefibrillar A{beta} species. Black-Right-Pointing-Pointer Fluorescence measurements using a combined set of fluorophores. Black-Right-Pointing-Pointer Morphology studies using transmission electron microscopy. -- Abstract: The formation of amyloid-{beta} peptide (A{beta}) aggregates at an early stage during the self-assembly process is an important factor in the development of Alzheimer's disease. The toxic effect is believed to be exerted by prefibrillar species of A{beta}. It is therefore important to identify which prefibrillar species are toxic and characterize their distinct properties. In the present study, we investigated the in vitro aggregation behavior of A{beta}-derived peptides possessing different levels of neurotoxic activity, using fluorescence spectroscopy in combination with transmission electron microscopy. The toxicity of various A{beta} aggregates was assessed by using cultures of human neuroblastoma cells. Through combined use of the fluorescence probe 8-anilino-1-napthalenesulfonate (ANS) and the novel luminescent probe pentamer formyl thiophene acetic acid (p-FTAA), we were able to identify those A{beta} peptide-derived prefibrillar species which exhibited cellular toxicity. In particular, species, which formed early during the aggregation process and showed strong p-FTAA and ANS fluorescence, were the species that possessed toxic activities. Moreover, by manipulating the aggregation conditions, it was possible to change the capacity of the A{beta} peptide to form nontoxic versus toxic species.

  18. Questions Students Ask: Beta Decay. (United States)

    Koss, Jordan; Hartt, Kenneth


    Answers a student's question about the emission of a positron from a nucleus. Discusses the problem from the aspects of the uncertainty principle, beta decay, the Fermi Theory, and modern physics. (YP)

  19. Beta Function and Anomalous Dimensions

    CERN Document Server

    Pica, Claudio


    We demonstrate that it is possible to determine the coefficients of an all-order beta function linear in the anomalous dimensions using as data the two-loop coefficients together with the first one of the anomalous dimensions which are universal. The beta function allows to determine the anomalous dimension of the fermion masses at the infrared fixed point, and the resulting values compare well with the lattice determinations.

  20. Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same

    Energy Technology Data Exchange (ETDEWEB)

    Morant, Marc


    The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

  1. Radiopolymerization of {beta}(-)pinene: A case of chiral amplification

    Energy Technology Data Exchange (ETDEWEB)

    Cataldo, Franco [Soc. Lupi Chemical Research, Via Casilina 1626/A, 00133 Rome (Italy)]. E-mail:; Keheyan, Yeghis [CNR, Istituto per lo studio dei Materiali Nanostrutturati, Department of Chemistry, University ' La Sapienza' , P.le Aldo Moro 1, Rome (Italy)


    {beta}(-)Pinene was treated with {gamma} radiation at three dose levels: 150, 300 and 600 kGy. The expected effect of radiation at these high doses was the partial racemization of the substrate as already observed in the case of other terpene monomers. Unexpectedly {beta}(-)pinene underwent a radiopolymerization reaction into a solid resin and into a dimer. The structure of the products was studied by FT-IR spectroscopy also in comparison to a reference {beta}(-)pinene resin prepared by cationic polymerization. A highly ordered structure was found in the case of the radiopolymer in comparison to the resin from cationic polymerization. Polarimetric measurements have shown astonishing enhancement in the optical activity of the radiopolymer and radiodimer in comparison to the starting optical activity of the {beta}(-)pinene monomer. The results have been discussed in terms of amplification of chirality caused by {gamma} radiation and the implications of this fact on the mechanism of chiral amplification on prebiotic molecules.

  2. Laser spectroscopy used in nuclear physics; La spectroscopie laser appliquee a la physique nucleaire

    Energy Technology Data Exchange (ETDEWEB)

    Le Blanc, F


    The study of nuclear shapes is a basic topic since it constitutes an excellent ground for testing and validating nuclear models. Measurements of the electron quadrupolar moment, of the nuclear charge radius and of the magnetic dipolar moment shed light on the nuclear deformation. Laser spectroscopy is a specific tool for such measurements, it is based on the interaction of the nucleus with the surrounding electron cloud (hyperfine structure), it is then an external approach of the shape of the nucleus whereas the classical nuclear spectroscopy ({alpha}, {beta} or {gamma}) gives information on the deformation from the inside of the nucleus. The author describes 2 techniques of laser spectroscopy: the colinear spectroscopy directly applied to a beam issued from an isotope separator and the resonant ionization spectroscopy linked with atom desorption that allows the study of particular nuclei. In order to illustrate both methods some effective measurements are presented: - the colinear spectroscopy has allowed the achievement of the complete description of the isomeric state (T = 31 years) of hafnium-178; - The experiment Complis has revealed an unexpected even-odd zigzag effect on very neutron-deficient platinum isotopes; and - the comparison of 2 isotopes of gold and platinum with their isomers has shown that the inversion of 2 levels of neutron, that was found out by nuclear spectroscopy, is in fact a consequence of a change in the nuclear shape. (A.C.)

  3. Chiroptical Spectroscopy (United States)

    Gurst, Jerome E.


    A brief review of the literature, and Chemical and Engineering News in particular, reveals that the determination and use of optical activity is of increasing importance in today's commercial and research laboratories. The classical technique is to measure [alpha]D using a manual or recording polarimeter to provide a single value, the specific rotation at 589 nm. A spectropolarimeter can be used to determine optical activity through the UV-Visible spectrum (Optical Rotatory Dispersion [ORD]). At wavelengths far removed from electronic absorption bands, optical activity arises from circular birefringence, or the difference in the refractive index for left- and right-circularly polarized light; i.e., nL - nR does not equal zero for chiral materials. If the optical activity is measured through an absorption band, complex behavior is observed (a Cotton Effect curve). At an absorption band, chiral materials exhibit circular dichroism (CD), or a difference in the absorption of left- and right-circularly polarized light; epsilon L minus epsilon R does not equal zero. If the spectropolarimeter is set for the measurement of CD spectra, one observes what appears to be a UV-Vis spectrum except that some absorption bands are positive while others may be negative. Just as enantiomers have specific rotations that are equal and opposite at 589 nm (sodium D line), rotations are equal and opposite at all wavelengths, and CD measurements are equal and opposite at all wavelengths. Figure 1 shows the ORD curves for the enantiomeric carvones while Figure 2 contains the CD curves. The enantiomer of carvone that has the positive [alpha]D is obtained from caraway seeds and is known to have the S-configuration while the R-enantiomer is found in spearmint oil. Figure 1. ORD of S-(+)- and R-(-)-carvones Figure 2. CD of S-(+)- and R-(-)-carvones While little can be done to correlate stereochemistry with [alpha]D values, chiroptical spectroscopy (ORD and/or CD) often can be used to assign

  4. Beta-decay total absorption measurements for nuclear technology and astrophysics

    Energy Technology Data Exchange (ETDEWEB)

    Taina, J.L. [Instituto de Fisica Corpuscular, C.S.I.C.-U. Valencia (Spain)


    An accurate determination of the beta-decay intensity distribution is of importance for basic nuclear physics but also in the fields of reactor technology, astrophysics and fundamental interactions. Most of present information comes from experiments performed with high resolution low efficiency Ge {gamma}-ray spectroscopy, which fails to locate the beta intensity at high excitation energies in complex decays. The total absorption spectroscopy technique with large 4{pi} scintillation detectors can give the correct answer but requires an involved procedure in order to extract the information. Recent work on the systematization of the analysis methods and on the evaluation of the associated systematic uncertainties shows that reliable beta strength distributions can indeed be obtained. (author)

  5. Inclusion complexes of pyrimethamine in 2-hydroxypropyl-beta-cyclodextrin: characterization, phase solubility and molecular modelling. (United States)

    de Araújo, Márcia Valéria Gaspar; Vieira, Elze Kelly Barbosa; Lázaro, Gilderman Silva; de Souza Conegero, Leila; Ferreira, Odair Pastor; Almeida, Lui S Eduardo; Barreto, Ledjane Silva; da Costa, Nivan Bezerra; Gimenez, Iara F


    The inclusion complexation of pyrimethamine in 2-hydroxypropyl-beta-cyclodextrin has been investigated by 2D (1)H NMR, FTIR and UV/visible spectroscopy and also by molecular modelling methods (AM1, PM3, MM3). From the phase-solubility diagram a linear increase was observed in pyrimethamine aqueous solubility in the presence of 2-hydroxypropyl-beta-cyclodextrin, evidencing the formation of a soluble inclusion complex. According to the continuous variation method (Job's plot) applied to fluorescence measurements, a 1:1 stoichiometry has been proposed for the complex. Concerning the structure of the complex, a Cl-in orientation of pyrimethamine in the 2-hydroxypropyl-beta-cyclodextrin cavity has been proposed from the theoretical calculations, being confirmed by two-dimensional (1)H NMR spectroscopy (ROESY). The thermal behaviour has also been studied, providing complementary evidences of complex formation.

  6. The GERDA experiment on 0{nu}{beta}{beta} decay

    Energy Technology Data Exchange (ETDEWEB)

    Freund, Kai [Eberhard Karls Universitaet Tuebingen (Germany); Collaboration: GERDA-Collaboration


    The Gerda (Germanium Detector Array) collaboration searches for the neutrinoless double beta decay (0{nu}{beta}{beta}) of {sup 76}Ge. The existence of this decay would give rise to the assumption that the neutrino is a Majorana particle, i.e. its own antiparticle. A measured half-life could be used to determine the effective neutrino mass and hence resolve the neutrino mass hierarchy problem. Germanium diodes, isotopically enriched in {sup 76}Ge, are used as both source and detector. Due to the low rate of this decay (T{sub 1/2}>10{sup 25} y), the experimental background must be reduced to a level of 10{sup -2}counts/(kg y keV) or better in the region around Q{sub {beta}{beta}}. To minimize background from cosmogenically produced secondary particles, a low Z shielding is employed. Thus, the naked diodes are operated in a liquid argon cryostat, which is surrounded by a water tank acting as both passive shield and active muon Cherenkov veto. Gerda started the commissioning runs in 2010 and in November 2011, the first phase of data taking with enriched detectors has begun. In this talk, the first year of the experiment is summarized.

  7. Beta-Thioxoketones

    DEFF Research Database (Denmark)

    Carlsen, Lars; Duus, Fritz


    ,π*), 415 (OC=CC=S π,π*), and 520 nm (C=S n,π*), respectively. The β-thioxoketones are converted by sodium hydroxide into the corresponding anions. CNDO/B Calculations predict that the negative charge in the β-thioxoketonates is delocalized over the OCCCS system, suggesting simultaneously sickles or W...... shaped conformations. Two characteristic absorption bands found for the β-thioxoketonates at ca. 275 and 400 nm are assigned to π,π* transitions involving the Ar–C[horiz bar, triple dot above]C[horiz bar, triple dot above]C–Ar′ and S[horiz bar, triple dot above]C[horiz bar, triple dot above]C[horiz bar......, triple dot above]C[horiz bar, triple dot above]O chromophores, respectively. The enol–enethiol tautomeric equilibrium has been studied by means of low temperature spectroscopy. At room temperature equilibrium constants (K293) of 3–5 have been found corresponding to a 4 : 1 enol–enethiol concentration...

  8. Supersymmetric models with tan$\\beta$ close to unity

    CERN Document Server

    Ananthanarayan, B; Shafi, Qaisar


    Within the framework of supersymmetric grand unification, estimates of the $b$ quark mass based on the asymptotic relation $m_b \\simeq m_\\tau$ single out the region with $\\tan\\beta$ close to unity, particularly if $m_t(m_t) \\stackrel{_<}{_\\sim} 170\\ GeV$. We explore the radiative breaking of the electroweak symmetry and the associated sparticle and higgs spectroscopy in models with $1 < \\tan\\beta \\stackrel{_<}{_\\sim} 1.6$. The lightest scalar higgs is expected to have a mass below $100\\ GeV$, while the remaining four higgs masses exceed $300\\ GeV$. The lower bounds on some of the sparticle masses are within the range of LEP 200.

  9. Analysis of betaS and betaA genes in a Mexican population with African roots. (United States)

    Magaña, María Teresa; Ongay, Zoyla; Tagle, Juan; Bentura, Gilberto; Cobián, José G; Perea, F Javier; Casas-Castañeda, Maricela; Sánchez-López, Yoaly J; Ibarra, Bertha


    To investigate the origin of the beta(A) and beta(S) genes in a Mexican population with African roots and a high frequency of hemoglobin S, we analyzed 467 individuals (288 unrelated) from different towns in the states of Guerrero and Oaxaca in the Costa Chica region. The frequency of the sickle-cell trait was 12.8%, which may represent a public health problem. The frequencies of the beta-haplotypes were determined from 350 nonrelated chromosomes (313 beta(A) and 37 beta(S)). We observed 15 different beta(A) haplotypes, the most common of which were haplotypes 1 (48.9%), 2 (13.4%), and 3 (13.4%). The calculation of pairwise distributions and Nei's genetic distance analysis using 32 worldwide populations showed that the beta(A) genes are more closely related to those of Mexican Mestizos and North Africans. Bantu and Benin haplotypes and haplotype 9 were related to the beta(S) genes, with frequencies of 78.8, 18.2, and 3.0%, respectively. Comparison of these haplotypes with 17 other populations revealed a high similitude with the population of the Central African Republic. These data suggest distinct origins for the beta(A) and beta(S) genes in Mexican individuals from the Costa Chica region.

  10. beta (+)-Thalassaemia in the Po river delta region (northern Italy): genotype and beta globin synthesis. (United States)

    Del Senno, L; Pirastu, M; Barbieri, R; Bernardi, F; Buzzoni, D; Marchetti, G; Perrotta, C; Vullo, C; Kan, Y W; Conconi, F


    Six beta(+)-thalassaemic patients from the Po river delta region have been studied. Using synthetic oligonucleotides as specific hybridisation probes, the beta(+) IVS I mutation (G----A at position 108) was demonstrated. This lesion and the enzyme polymorphism pattern in the subjects examined are the same as have been described for other Mediterranean beta(+)-thalassaemias. Antenatal diagnosis through DNA analysis of beta(+)-thalassaemia is therefore possible. The production of beta globin in a beta(+), homozygote and in a beta (+), beta(0) 39 (nonsense mutation at codon 39) double heterozygote is approximately 20% and 10% respectively of total non-alpha globin synthesis. Despite some overlapping of the results, similar beta globin synthesis levels have been obtained in 43 beta(+)-thalassaemia patients. This suggests that in the Po river delta region the most common thalassaemic genes are beta(0) 39 and beta(+) IVS I. Images PMID:2580095

  11. Inclusion complexation of novocaine by beta-cyclodextrin in aqueous solutions. (United States)

    Iglesias, Emilia


    The formation of inclusion complexes between beta-cyclodextrin (beta-CD) and the local anesthetic 2-(diethylamino)ethyl-p-amino-benzoate (novocaine) in aqueous solutions under different acidity conditions, using steady-state fluorescence or UV-vis spectroscopies, electrical conductivity, or the kinetic study of both the nitrosation reaction of the primary amine group in a mild acid medium and the hydrolysis of the ester function under an alkaline medium, has been studied. The inclusion complex formation between neutral or protonated novocaine and beta-CD of 1:1 stoichiometry was observed; however, the magnitude of the binding constants depends on the nature of both the guest and the host, and the higher-affinity guest-host was found under conditions when both the novocaine and the beta-CD were neutral molecules.

  12. Optical Spectroscopy of Four Young Radio Sources

    CERN Document Server

    Fan, Xu-Liang; Hu, Chen; Wang, Jian-Guo


    We report the optical spectroscopy of four young radio sources which are observed with the Lijiang 2.4m telescope. The Eddington ratios of these sources are similar with those of narrow-line Seyfert 1 galaxies (NLS1s). Their Fe {\\sc ii} emission is strong while [O {\\sc iii}] strength is weak. These results confirm the NLS1 features of young radio sources, except that the width of broad H$\\beta$ of young radio sources is larger than that of NLS1s. We thus suggest that the young radio sources are the high black hole mass counterparts of steep-spectrum radio-loud NLS1s. In addition, the broad H$\\beta$ component of \\astrobj{4C 12.50} is the blue wing of the narrow component, but not from the broad line region.

  13. Study of the ${\\beta}$-decay of $^{12}$B

    CERN Multimedia


    We propose to study the ${\\beta}$-decay of $^{12}$B with a modern segmented Si-detector array to get new and much improved information on states in $^{12}$C above the ${\\alpha}$-threshold. These states mainly decay into final states of three ${\\alpha}$-particles and their study therefore is a challenge for nuclear spectroscopy. The properties of these states is of high current interest for nuclear astrophysics and for the nuclear many-body problem in general. We ask for a total of 15 shifts.

  14. Carbon-nitrogen place exchange on NO exposed beta-Mo2C. (United States)

    Siaj, Mohamed; Maltais, Carl; Zahidi, El Mamoune; Oudghiri-Hassani, Hicham; Wang, Jiqing; Rosei, Federico; McBreen, Peter H


    Atomic nitrogen and oxygen were deposited on beta-Mo(2)C through dissociative adsorption of NO. Reflectance absorbance infrared spectroscopy (RAIRS), thermal desorption, and synchrotron X-ray photoelectron spectroscopy (XPS) measurements were used to investigate the interplay between atomic nitrogen, carbon, and oxygen in the 400-1250 K region. The combination of the high resolution and high surface sensitivity offered by the synchrotron XPS technique was used to show that atomic nitrogen displaces interstitial carbon onto the carbide surface. Thermal desorption measurements show that the burnoff of the displaced carbon occurs at approximately 890 K. The incorporation of nitrogen into interstitial sites inhibits oxygen dissolution into the bulk. RAIRS spectroscopy was used to identify surface oxo, terminal oxygen, species formed from O(2) and NO on beta-Mo(2)C.

  15. Smart Beta or Smart Alpha

    DEFF Research Database (Denmark)

    Winther, Kenneth Lillelund; Steenstrup, Søren Resen


    Smart beta has become the flavor of the decade in the investment world with its low fees, easy access to rewarded risk premiums, and appearance of providing good investment results relative to both traditional passive benchmarks and actively managed funds. Although we consider it well documented...... that smart beta investing probably will do better than passive market capitalization investing over time, we believe many are coming to a conclusion too quickly regarding active managers. Institutional investors are able to guide managers through benchmarks and risk frameworks toward the same well......-documented smart beta risk premiums and still motivate active managers to avoid value traps, too highly priced small caps, defensives, etc. By constructing the equity portfolios of active managers that resemble the most widely used risk premiums, we show that the returns and risk-adjusted returns measures...

  16. Syntheses of [F5TeNH3][AsF6], [F5TeN(H)Xe][AsF6], and F5TeNF2 and characterization by multi-NMR and Raman spectroscopy and by electronic structure calculations: the X-ray crystal structures of alpha- and beta-F5TeNH2, [F5TeNH3][AsF6], and [F5TeN(H)Xe][AsF6]. (United States)

    Fir, Barbara; Whalen, J Marc; Mercier, Hélène P A; Dixon, David A; Schrobilgen, Gary J


    The salt, [F5TeN(H)Xe][AsF6], has been synthesized in the natural abundance and 99.5% 15N-enriched forms. The F5TeN(H)Xe+ cation has been obtained as the product of the reactions of [F5TeNH3][AsF6] with XeF2 (HF and BrF5 solvents) and F5TeNH2 with [XeF][AsF6] (HF solvent) and characterized in solution by 129Xe, 19F, 125Te, 1H, and 15N NMR spectroscopy at -60 to -30 degrees C. The orange [F5TeN(H)Xe][AsF6] and colorless [F5TeNH3][AsF6] salts were crystallized as a mixture from HF solvent at -35 degrees C and were characterized by Raman spectroscopy at -165 degrees C and by X-ray crystallography. The crystal structure of the low-temperature phase, alpha-F5TeNH2, was obtained by crystallization from liquid SO2 between -50 and -70 degrees C and is fully ordered. The high-temperature phase, beta-F5TeNH2, was obtained by sublimation at room temperature and exhibits a 6-fold disorder. Decomposition of [F5TeN(H)Xe][AsF6] in the solid state was rapid above -30 degrees C. The decomposition of F5TeN(H)Xe+ in HF and BrF5 solution at -33 degrees C proceeded by fluorination at nitrogen to give F5TeNF2 and Xe gas. Electronic structure calculations at the Hartree-Fock and local density-functional theory levels were used to calculate the gas-phase geometries, charges, Mayer bond orders, and Mayer valencies of F5TeNH2, F5TeNH3+, F5TeN(H)Xe+, [F5TeN(H)Xe][AsF6], F5TeNF2, and F5TeN2- and to assign their experimental vibrational frequencies. The F5TeN(H)Xe+ and the ion pair, [F5TeN(H)Xe][AsF6], systems were also calculated at the MP2 and gradient-corrected (B3LYP) levels.

  17. The microbial oxidation of (-)-beta-pinene by Botrytis cinerea. (United States)

    Farooq, Afgan; Choudhary, M Iqbal; Tahara, Satoshi; Rahman, Atta-ur; Başer, K Hüsnü Can; Demirci, Fatih


    (-)-beta-pinene, a flavor and fragrance monoterpene is an important constituent of essential oils of many aromatic plants. It was oxidized by a plant-pathogenic fungus, Botrytis cinerea to afford four metabolites characterized as (-)-6a-hydroxy-beta-pinene, (-)-4beta,5beta-dihydroxy-beta-pinene, (-)-2beta,3beta-dihydroxypinane, and (-)-4beta-hydroxy-beta-pinene-6-one by detailed spectroscopic studies along with other known metabolites.

  18. Neutrinoless double beta decay experiments

    CERN Document Server

    Zuber, K


    The study of neutrinoless double beta decay is of outmost importance for neutrino physics. It is considered to be the gold plated channel to probe the fundamental character of neutrinos and to determine the neutrino mass. From the experimental point about nine different isotopes are explored for the search. After a general introduction follows a short discussion on nuclear matrix element calculations and supportive measurements. The current experimental status of double beta searches is presented followed by a short discussion of the ideas and proposals for large scale experiments.

  19. A {beta} - {gamma} coincidence; Metodo de coincidencias {beta} - {gamma}

    Energy Technology Data Exchange (ETDEWEB)

    Agullo, F.


    A {beta} - {gamma} coincidence method for absolute counting is given. The fundamental principles are revised and the experimental part is detailed. The results from {sup 1}98 Au irradiated in the JEN 1 Swimming pool reactor are given. The maximal accuracy is 1 per cent. (Author) 11 refs.

  20. Beta thalassaemia mutations in Turkish Cypriots. (United States)

    Sozuoz, A; Berkalp, A; Figus, A; Loi, A; Pirastu, M; Cao, A


    Using oligonucleotide hybridisation or restriction endonuclease analysis, we have characterised the molecular defect in 94 patients with thalassaemia major and four with thalassaemia intermedia of Turkish Cypriot descent. We found that four mutations, namely beta+ IVS-1 nt 110, beta zero IVS-1 nt, beta+ IVS-1 nt 6, and beta+ IVS-2 nt 745 were prevalent, accounting for 69.9%, 11.7%, 8.7%, and 5.6% respectively of the beta thalassaemia chromosomes. This information may help in the organisation of a large scale prevention programme based on fetal diagnosis of beta thalassaemia by DNA analysis in the Turkish population. PMID:3236356

  1. Angle-Resolved Spectroscopy of Parametric Fluorescence

    CERN Document Server

    Hsu, Feng-kuo


    The parametric fluorescence from a nonlinear crystal forms a conical radiation pattern. We measure the angular and spectral distributions of parametric fluorescence in a beta-barium borate crystal pumped by a 405-nm diode laser employing angle-resolved imaging spectroscopy. The experimental angle-resolved spectra and the generation efficiency of parametric down conversion are compared with a plane-wave theoretical analysis. The parametric fluorescence is used as a broadband light source for the calibration of the instrument spectral response function in the wavelength range from 450 to 1000 nm.

  2. Gas-silicon detector telescope for charged particle spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Honkanen, A.; Oinonen, M.; Aeystoe, J. [Jyvaeskylae Univ. (Finland). Dept. of Physics; Eskola, K. [Helsinki Univ. (Finland). Dept. of Phys.; Jokinen, A. [PPE Division, CERN, CH-1211 Geneva 23 (Switzerland); ISOLDE Collaboration


    A gas-silicon detector telescope for charged particle spectroscopy has been constructed and tested. The lower detection limits were determined to be 155 keV for protons, 180 keV for deuterons and 350 keV for alpha particles. Typical energy resolution of the telescope measured for beta-delayed protons is 20 keV. Time resolution for the signals of the telescope was measured to be less than 10 ns. Examples of using the detector telescope in detection of beta-delayed proton activities are presented. (orig.).

  3. Advances in atomic spectroscopy

    CERN Document Server

    Sneddon, J


    This series describes selected advances in the area of atomic spectroscopy. It is primarily intended for the reader who has a background in atmoic spectroscopy; suitable to the novice and expert. Although a widely used and accepted method for metal and non-metal analysis in a variety of complex samples, Advances in Atomic Spectroscopy covers a wide range of materials. Each Chapter will completely cover an area of atomic spectroscopy where rapid development has occurred.

  4. Basic molecular spectroscopy

    CERN Document Server

    Gorry, PA


    BASIC Molecular Spectroscopy discusses the utilization of the Beginner's All-purpose Symbolic Instruction Code (BASIC) programming language in molecular spectroscopy. The book is comprised of five chapters that provide an introduction to molecular spectroscopy through programs written in BASIC. The coverage of the text includes rotational spectra, vibrational spectra, and Raman and electronic spectra. The book will be of great use to students who are currently taking a course in molecular spectroscopy.

  5. Advances in atomic spectroscopy

    CERN Document Server

    Sneddon, J


    This series describes selected advances in the area of atomic spectroscopy. It is promarily intended for the reader who has a background in atmoic spectroscopy; suitable to the novice and expert. Although a widely used and accepted method for metal and non-metal analysis in a variety of complex samples, Advances in Atomic Spectroscopy covers a wide range of materials. Each Chapter will completely cover an area of atomic spectroscopy where rapid development has occurred.

  6. Beta Cell Workshop 2013 Kyoto

    DEFF Research Database (Denmark)

    Heller, R Scott; Madsen, Ole D; Nielsen, Jens Høiriis


    The very modern Kyoto International Conference Center provided the site for the 8th workshop on Beta cells on April 23-26, 2013. The preceding workshops were held in Boston, USA (1991); Kyoto, Japan (1994); Helsingør, Denmark (1997); Helsinki, Finland (2003); El Perello, Spain (2006); Peebles...

  7. Estimating $\\beta$-mixing coefficients

    CERN Document Server

    McDonald, Daniel J; Schervish, Mark


    The literature on statistical learning for time series assumes the asymptotic independence or ``mixing' of the data-generating process. These mixing assumptions are never tested, nor are there methods for estimating mixing rates from data. We give an estimator for the $\\beta$-mixing rate based on a single stationary sample path and show it is $L_1$-risk consistent.

  8. Beta-carotene as antioxidant

    NARCIS (Netherlands)

    Bast, A.; Plas, R.M. van der; Berg, H. van den; Haenen, G.R.M.M.


    Objective: Beta-carotene has been shown to exhibit a good radical-trapping antioxidant activity in vitro. We were interested to see if dietary β-carotene in combination with various intake levels for vitamin A would also inhibit lipid peroxidation. Design: Sixty male Wistar rats received vitamin A (

  9. Symposium on atomic spectroscopy

    Energy Technology Data Exchange (ETDEWEB)


    Topics covered by the conference include: fast beam spectroscopy; astrophysical and other spectra; highly ionized spectroscopy; complex spectra; rydberg levels; fine structure, hyperfine structure and isotope shift; lineshapes; lifetimes, oscillator strengths and Einstein coefficients; and spectroscopy with lasers. Abstracts of the conference papers are presented. (GHT)

  10. Neutron Beta Decay Studies with Nab

    CERN Document Server

    Baeßler, S; Alonzi, L P; Balascuta, S; Barrón-Palos, L; Bowman, J D; Bychkov, M A; Byrne, J; Calarco, J R; Chupp, T; Vianciolo, T V; Crawford, C; Frlež, E; Gericke, M T; Glück, F; Greene, G L; Grzywacz, R K; Gudkov, V; Harrison, D; Hersman, F W; Ito, T; Makela, M; Martin, J; McGaughey, P L; McGovern, S; Page, S; Penttilä, S I; Počanić, D; Rykaczewski, K P; Salas-Bacci, A; Tompkins, Z; Wagner, D; Wilburn, W S; Young, A R


    Precision measurements in neutron beta decay serve to determine the coupling constants of beta decay and allow for several stringent tests of the standard model. This paper discusses the design and the expected performance of the Nab spectrometer.

  11. Hydrolytic and photochemistry degradation of the amoxicillin in {beta}-cyclodextrin; Degradacao hidrolitica e fotoquimica da amoxicilina na presenca de {beta}-ciclodextrina

    Energy Technology Data Exchange (ETDEWEB)

    Bariccatti, R.A.; Silva, C.; Souza, M.L.; Lindino, C.A.; Rosa, M.F. [Universidade Estadual do Oeste do Parana, Toledo, PR (Brazil). Centro de Engenharias e Ciencias Exatas]. E-mail:


    This work has like purpose monitors the degradation of the drug amoxicillin in the presence and absence of {beta}-cyclodextrin, through techniques spectroscopy. For this, there was accompanied the hydrolysis of the drug protected of the light for around 400 hours. The results indicate that, initially, the cyclodextrin does not alter the hydrolysis of the amoxicillin, however, after 250 hours there is an increase of the hydrolysis of the amoxicillin when present at cyclodextrin. Another variable was the irradiation of the sample with radiation in the region of the UV, we see that the solutions containing {beta}- cyclodextrin suffer a slower phototransformation (26,8%) than the solutions without {beta}-cyclodextrin, when irradiated by UV radiation. (author)

  12. The beta-decay of Al-22

    NARCIS (Netherlands)

    Achouri, NL; Santos, FDO; Lewitowicz, M; Blank, B; Aysto, J; Canchel, G; Czajkowski, S; Dendooven, P; Emsallem, A; Giovinazzo, J; Guillet, N; Jokinen, A; Larid, AM; Longour, C; Perajarvi, K; Smirnova, N; Stanoiu, M


    In an experiment performed at the LISE3 facility of GANIL, we studied the decay of Al-22 produced by the fragmentation of a Ar-36 primary beam. A beta-decay half-life of T-1/2 = 91.1 +/- 0.5ms was measured. The beta-delayed one- and two-proton emission as well as beta-alpha and beta-delayed gamma-de

  13. Traumatic Brain Injury, Microglia, and Beta Amyloid


    Mannix, Rebekah C.; Whalen, Michael J


    Recently, there has been growing interest in the association between traumatic brain injury (TBI) and Alzheimer's Disease (AD). TBI and AD share many pathologic features including chronic inflammation and the accumulation of beta amyloid (A\\(\\beta\\)). Data from both AD and TBI studies suggest that microglia play a central role in A\\(\\beta\\) accumulation after TBI. This paper focuses on the current research on the role of microglia response to A\\(\\beta\\) after TBI.


    NARCIS (Netherlands)



    We have identified a new protein fold-the alpha/beta-hydrolase fold-that is common to several hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The core of each enzyme is similar: an alpha/beta-sheet, not barrel, of eight beta-sheets connected by alpha-helices. These

  15. Beta-lactamases in Enterobacteriaceae in broilers

    NARCIS (Netherlands)

    Dierikx, C.M.


    Resistance to cephalosprins due to the production of extended spectrum beta-lactamases (ESBLs) or plasmid mediated AmpC beta-lactamases is increasingly found in infections in humans outside the hospital. The genes encoding for these beta-lactamases are located on mobile DNA (plasmids), which can be

  16. Higher-Order Beta Matching with Solutions in Long Beta-Eta Normal Form

    DEFF Research Database (Denmark)

    Støvring, Kristian


    up to beta-eta equivalence is a long-standing open problem.We show that higher-order matching up to beta-eta equivalence is decidable if and only if a restricted form of higher-order matching up to beta equivalence is decidable: the restriction is that solutions must be in long beta-eta normal form....

  17. Coherent Raman spectroscopy

    CERN Document Server

    Eesley, G L


    Coherent Raman Spectroscopy provides a unified and general account of the fundamental aspects of nonlinear Raman spectroscopy, also known as coherent Raman spectroscopy. The theoretical basis from which coherent Raman spectroscopy developed is described, along with its applications, utility, and implementation as well as advantages and disadvantages. Experimental data which typifies each technique is presented. This book is comprised of four chapters and opens with an overview of nonlinear optics and coherent Raman spectroscopy, followed by a discussion on nonlinear transfer function of matter

  18. Advances in atomic spectroscopy

    CERN Document Server

    Sneddon, J


    This fifth volume of the successful series Advances in Atomic Spectroscopy continues to discuss and investigate the area of atomic spectroscopy.It begins with a description of the use of various atomic spectroscopic methods and applications of speciation studies in atomic spectroscopy. The emphasis is on combining atomic spectroscopy with gas and liquid chromatography. In chapter two the authors describe new developments in tunable lasers and the impact they will have on atomic spectroscopy. The traditional methods of detection, such as photography and the photomultiplier, and how they are being replaced by new detectors is discussed in chapter three. The very active area of glow discharge atomic spectrometry is presented in chapter four where, after a brief introduction and historical review, the use of glow discharge lamps for atomic spectroscopy and mass spectrometry are discussed. Included in this discussion is geometry and radiofrequency power. The future of this source in atomic spectroscopy is also dis...

  19. Cerebral oxygenation decreases during exercise in humans with beta-adrenergic blockade

    DEFF Research Database (Denmark)

    Seifert, T.; Rasmussen, P.; Secher, Niels H.


    AIM: Beta-blockers reduce exercise capacity by attenuated increase in cardiac output, but it remains unknown whether performance also relates to attenuated cerebral oxygenation. METHODS: Acting as their own controls, eight healthy subjects performed a continuous incremental cycle test to exhaustion...... with or without administration of the non-selective beta-blocker propranolol. Changes in cerebral blood flow velocity were measured with transcranial Doppler ultrasound and those in cerebral oxygenation were evaluated using near-infrared spectroscopy and the calculated cerebral mitochondrial oxygen tension...

  20. Modification of polyethylene films by radiation grafting of glycidyl methacrylate and immobilization of {beta}-cyclodextrin

    Energy Technology Data Exchange (ETDEWEB)

    Nava-Ortiz, C.A.B. [Departamento de Quimica de Radiaciones y Radioquimica, Instituto de Ciencias Nucleares, Universidad Nacional Autonoma de Mexico, Circuito Exterior, Ciudad Universitaria, Mexico DF 04510 (Mexico); Burillo, G. [Departamento de Quimica de Radiaciones y Radioquimica, Instituto de Ciencias Nucleares, Universidad Nacional Autonoma de Mexico, Circuito Exterior, Ciudad Universitaria, Mexico DF 04510 (Mexico)], E-mail:; Bucio, E. [Departamento de Quimica de Radiaciones y Radioquimica, Instituto de Ciencias Nucleares, Universidad Nacional Autonoma de Mexico, Circuito Exterior, Ciudad Universitaria, Mexico DF 04510 (Mexico); Alvarez-Lorenzo, C. [Departamento de Farmacia y Tecnologia Farmaceutica, Facultad de Farmacia, Universidad de Santiago de Compostela, 15782 Santiago de Compostela (Spain)


    Glycidyl methacrylate was grafted onto polyethylene films using a preirradiation method with {gamma} rays. The effect of absorbed dose, monomer concentration, and reaction time on the degree of grafting was determined. The grafted samples were verified by FTIR-ATR spectroscopy. {beta}-Cyclodextrin was immobilized onto polypropylene modified with glycidyl methacrylate, and the ability of the cavities of {beta}-cyclodextrin to form inclusion complexes was demonstrated using the typically organic compound approach with m-toluic acid (3-MBA) as a probe.

  1. Study of neutron-rich $^{51−53}$ Ca isotopes via $\\beta$-decay

    CERN Multimedia

    The high Q$_\\beta$ values in certain neutron-rich regions of the chart of nuclides opens up the possibility to study states in the daughter nuclei which lie at high excitation energy, above the neutron separation threshold. We propose to perform spectroscopy of the $\\beta$-delayed neutron emission of the $^{51-53}$K isotopes to study the population of single-particle or particle-hole states both below and above the neutron separation threshold. The VANDLE neutron detector will be used in combination with the IDS tape station setup and Ge detectors.

  2. beta (+)-Thalassaemia in the Po river delta region (northern Italy): genotype and beta globin synthesis.


    del Senno, L; Pirastu, M; Barbieri, R.; De Bernardi, F.; Buzzoni, D; Marchetti, G.; Perrotta, C; Vullo, C; Kan, Y W; Conconi, F


    Six beta(+)-thalassaemic patients from the Po river delta region have been studied. Using synthetic oligonucleotides as specific hybridisation probes, the beta(+) IVS I mutation (G----A at position 108) was demonstrated. This lesion and the enzyme polymorphism pattern in the subjects examined are the same as have been described for other Mediterranean beta(+)-thalassaemias. Antenatal diagnosis through DNA analysis of beta(+)-thalassaemia is therefore possible. The production of beta globin in...

  3. COMPLIS: COllinear spectroscopy Measurements using a Pulsed Laser Ion Source

    CERN Multimedia


    A Pulsed Laser spectroscopy experiment has been installed for the study of hyperfine structure and isotope shift of refractory and daughter elements from ISOLDE beams. It includes decelerated ion-implantation, element-selective laser ionization, magnetic and time-of-flight mass separation. The laser spectroscopy has been performed on the desorbed atoms in a set-up at ISOLDE-3 but later on high resolution laser collinear spectroscopy with the secondary pulsed ion beam is planned for the Booster ISOLDE set-up. During the first operation time of ISOLDE-3 we restricted our experiments to Doppler-limited resonant ionization laser and $\\gamma$-$\\gamma$ nuclear spectroscopy on neutron deficient platinum isotopes of even mass number down to A~=~186 and A~=~179 respectively. These isotopes have been produced by implantation of radioactive Hg and their subsequent $\\beta$-decay.

  4. Specific Triazine Herbicides Induce Amyloid-beta(42) Production

    NARCIS (Netherlands)

    Portelius, Erik; Durieu, Emilie; Bodin, Marion; Cam, Morgane; Pannee, Josef; Leuxe, Charlotte; Mabondzo, Aloise; Oumata, Nassima; Galons, Herve; Lee, Jung Yeol; Chang, Young-Tae; Stuber, Kathrin; Koch, Philipp; Fontaine, Gaelle; Potier, Marie-Claude; Manousopoulou, Antigoni; Garbis, Spiros D.; Covaci, Adrian; Van Dam, Debby; De Deyn, Peter; Karg, Frank; Flajolet, Marc; Omori, Chiori; Hata, Saori; Suzuki, Toshiharu; Blennow, Kaj; Zetterberg, Henrik; Meijer, Laurent


    Proteolytic cleavage of the amyloid-beta protein precursor (A beta PP) ecretases leads to extracellular release of amyloid-beta (A beta) peptides. Increased production of A beta(42) over A beta(40) and aggregation into oligomers and plaques constitute an Alzheimer's disease (AD) hallmark. Identifyin

  5. Future double beta decay experiments

    Energy Technology Data Exchange (ETDEWEB)

    Piquemal, F. [Laboratoire Souterrain de Modane, Modane (France); Centre d' Etudes Nucleaire, Bordeaux-Gradignan (France)


    The search of neutrinoless double beta decay is very challenging because of the expected half-life of the process and the backgrounds from the natural radioactivity. Many projects exist to try to reach a sensitivity of ∼50 meV on the effective neutrino mass corresponding to a mass of isotopes of ∼100 kg. In this article some of the futur projects are presented.

  6. Myokardinfarkt und Beta-Blocker

    Directory of Open Access Journals (Sweden)

    Stühlinger H-G


    Full Text Available Im Rahmen eines akuten koronaren Syndroms (akuter Herzinfarkt, Angina pectoris kommt es, aufgrund eines Ungleichgewichtes zwischen Angebot und Bedarf, zu einem akuten Mangel an Sauerstoff im Herzmuskel. Ursache ist eine reduzierte Sauerstoffzufuhr durch verengte bzw. verschlossene Gefäße. Bis zur Behebung der Ursache vergehen oft mehrere Stunden. In dieser Phase muß - durch Verminderung des Sauerstoffbedarfs im Herzmuskel - eine Verlangsamung der Nekroseentwicklung erreicht werden. Das Ausmaß der Nekrose wird reduziert, somit die für die Langzeitprognose wichtige Linksventrikelfunktion verbessert. Eine Verminderung des Sauerstoffbedarfs erreicht man durch kontrollierte Frequenzsenkung mittels intravenöser Beta-Blockade. In optimaler Weise wird diese Methode durch die Anwendung eines kardioselektiven Beta-Blockers mit kurzer Halbwertszeit durchgeführt. Beta-Blocker haben nicht nur auf die Nekroseentwicklung, sondern auch auf die Inzidenz von Rhythmusstörungen - besonders in der Akutphase - Auswirkungen. Vor allem die mit dieser therapeutischen Maßnahme verbundene Reduktion von Kammerflimmern ist von großer Bedeutung.

  7. $\\beta$-particle energy-summing correction for $\\beta$-delayed proton emission measurements

    CERN Document Server

    Meisel, Z; Crawford, H L; Cyburt, R H; Grinyer, G F; Langer, C; Montes, F; Schatz, H; Smith, K


    A common approach to studying $\\beta$-delayed proton emission is to measure the energy of the emitted proton and corresponding nuclear recoil in a double-sided silicon-strip detector (DSSD) after implanting the $\\beta$-delayed proton emitting ($\\beta$p) nucleus. However, in order to extract the proton-decay energy, the measured energy must be corrected for the additional energy implanted in the DSSD by the $\\beta$-particle emitted from the $\\beta$p nucleus, an effect referred to here as $\\beta$-summing. We present an approach to determine an accurate correction for $\\beta$-summing. Our method relies on the determination of the mean implantation depth of the $\\beta$p nucleus within the DSSD by analyzing the shape of the total (proton + recoil + $\\beta$) decay energy distribution shape. We validate this approach with other mean implantation depth measurement techniques that take advantage of energy deposition within DSSDs upstream and downstream of the implantation DSSD.

  8. Ultrahigh spatiotemporal resolved spectroscopy

    Institute of Scientific and Technical Information of China (English)


    @@ We review the technique and research of the ultrahigh spatiotemporal resolved spectroscopy and its applications in the field of the ultrafast dynamics of mesoscopic systems and nanomaterials. Combining femtosecond time-resolved spectroscopy and scanning near-field optical microscopy (SNOM), we can obtain the spectra with ultrahigh temporal and spatial resolutions simultaneously. Some problems in doing so are discussed. Then we show the important applications of the ultrahigh spatiotemporal resolved spectroscopy with a few typical examples.

  9. Ultrahigh spatiotemporal resolved spectroscopy

    Institute of Scientific and Technical Information of China (English)

    LI; Zhi


    We review the technique and research of the ultrahigh spatiotemporal resolved spectroscopy and its applications in the field of the ultrafast dynamics of mesoscopic systems and nanomaterials. Combining femtosecond time-resolved spectroscopy and scanning near-field optical microscopy (SNOM), we can obtain the spectra with ultrahigh temporal and spatial resolutions simultaneously. Some problems in doing so are discussed. Then we show the important applications of the ultrahigh spatiotemporal resolved spectroscopy with a few typical examples.……

  10. Spectroscopy for Dummies

    DEFF Research Database (Denmark)

    Lindvold, Lars René

    This presentation will give short introduction to the most pertinent topics of optical spectroscopy. The following topics will be discussed: • The origin of spectra in UV, VIS and IR spectral range • Spectroscopic methods like absorption, luminescence and Raman • Wavelength dispersive optical...... components • Materials for use optical spectroscopy • Spectrometer geometries • Detectors for use in spectrometer • Practical examples of optical spectroscopy The objective of this presentation is to give the audience a good feel for the range of possibilities that optical spectroscopy can provide....

  11. Advances in DUV spectroscopy

    DEFF Research Database (Denmark)

    Buchhave, Preben; Tidemand-Lichtenberg, Peter; Mogensen, Claus Tilsted

    The would-be advantages of deep UV (DUV) spectroscopy are well known, but the potential applications have so far not been fully realized due to technological limitations and, perhaps, lack of bright ideas. However, new components and new knowledge about DUV spectra and spectroscopic methods...... combined with increasing needs for solutions to practical problems in environmental protection, medicine and pollution monitoring promise a new era in DUV spectroscopy. Here we shall review the basis for DUV spectroscopy, both DUV fluorescence and DUV Raman spectroscopy, and describe recent advances...

  12. Investigation of de novo cholesterol synthetic capacity in the gonads of goldfish (Carassius auratus) exposed to the phytosterol beta-sitosterol. (United States)

    Sharpe, Rainie L; Drolet, Melissa; MacLatchy, Deborah L


    Total and intra-mitochondrial gonadal cholesterol concentrations are decreased in fish exposed to the phytoestrogen beta-sitosterol (beta-sit). The present study examined the potential for beta-sit to disrupt de novo cholesterol synthesis in the gonads of goldfish exposed to 200 microgram/g beta-sit and 10 microgram/g 17beta-estradiol (E2; estrogenic control) by intra-peritoneal Silastic implants for 21 days. The de novo cholesterol synthetic capacity was estimated by incubating gonadal tissue with 14C-acetate for a period of 18 hours, followed by chloroform/methanol lipid extraction and thin layer chromatography (TLC) lipid separation. Lipid classes were confirmed using infrared spectroscopy. Plasma testosterone (T) and total cholesterol concentration were measured and gonadosomatic index (GSI) was calculated. Plasma T was significantly reduced in male beta-sit-treated fish compared to control and E2-treated fish (p fatty acids (FFA). FFA incorporation was significantly higher in male control fish than either beta-sit or E2 treatments (p = 0.005). Plasma cholesterol concentration was significantly increased in the male beta-sit treatment group compared to controls (p = 0.027). These results indicate gonadal de novo cholesterol biosynthetic capacity is not disrupted by beta-sit or E2 treatment in early recrudescing male or female goldfish, while plasma cholesterol and steroid concentrations are sensitive to beta-sit exposure.

  13. Reduced migration from flexible poly(vinyl chloride) of a plasticizer containing beta-cyclodextrin derivative. (United States)

    Yu, Ong Yong; Chung, Jae Woo; Kwak, Seung-Yeop


    The migration of endocrine-disrupting di-(2-ethylhexyl) phthalate (DEHP) poses a serious threat to public health and the environment. In this study, we successfully prepared a plasticizerwith reduced DEHP migration by directly incorporating 2,3,6-per-O-benzoyl-beta-cyclodextrin (Bz-beta-CD) into DEHP. Bz-beta-CD was prepared by esterification between the hydroxyl groups of beta-CD and benzoyl chloride. The presence of this cyclodextrin is expected to facilitate formation of stable complexes through pi-pi association with DEHP molecules. The flexible PVC was prepared with a gelation-fusion process that uses the prepared migration-resistant plasticizer, and its properties (flexibility, thermal stability, and clarity) were evaluated by carrying out DSC and tensile testing, TGA, and haze testing, respectively. No significant changes in the physical properties of the flexible PVC were observed when Bz-beta-CD was added. DEHP migration tests were carried out for the flexible PVC according to the ISO 3826:1993(E) test method, and the quantity of migrated DEHP was then determined with UV-vis spectroscopy. It was found that the addition of Bz-beta-CD decreases the levels of DEHP migration from the flexible PVC samples by almost 40%. We investigated the molecular interaction between Bz-beta-CD and DEHP using molecular mechanics simulations, and we conclude that this reduction in DEHP migration is due to the formation of stabilized pi-pi attractive association and inclusion complexes of Bz-beta-CD and DEHP in flexible PVC.

  14. Influence of the aluminium impregnation [ Al(NO33] in the beta zeolite over its acidity

    Directory of Open Access Journals (Sweden)

    Francisco José Sánchez Castellanos


    Full Text Available Beta zeolite was impregnated with [ Al(NO33], increasing the aluminium content in increments of 0.05% from 0.00% to 0.25%. A parallel treatment with 0.05% sulphuric acid was also performed; in both cases, methanol was used as solvent (disperse phase. Cation exchange capacity (CEC, ammonia chemisorption, infrared spectroscopy (FIT-IR, scanning electronic microscopy (SEM, X-Ray powder diffraction (XRD, atomic absorption spectroscopy (AAS, titration with sodium hydroxide and nitrogen physisorption at 77K were used to carry out the physical and chemical characterization of the catalysts. Futhermore, the catalysts were employed in the esterification of ethanol with acetic acid, to quantify the effect of aluminium impregnation over the beta zeolite.

  15. Sugar microarray via click chemistry: molecular recognition with lectins and amyloid {beta} (1-42)

    Energy Technology Data Exchange (ETDEWEB)

    Matsumoto, Erino; Fukuda, Tomohiro; Miura, Yoshiko [School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292 (Japan); Yamauchi, Takahiro, E-mail: [Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603 (Japan)


    Sugar microarrays were fabricated on various substrates via click chemistry. Acetylene-terminated substrates were prepared by forming self-assembled monolayers (SAMs) on a gold substrate with alkyl-disulfide and on silicon, quartz and glass substrates with a silane-coupling reagent. The gold substrates were subjected to surface plasmon resonance measurements, and the quartz and glass substrates were subjected to spectroscopy measurements and optical microscopy observation. The saccharide-immobilized substrate on the gold substrate showed specific interaction with the corresponding lectin, and the saccharides showed inert surface properties to other proteins with a high signal-to-noise ratio. We also focused on the saccharide-protein interaction on protein amyloidosis of Alzheimer amyloid {beta}. Amyloid {beta} peptide showed conformation transition on the saccharide-immobilization substrate into a {beta}-sheet, and fibril formation and amyloid aggregates were found on the specific saccharides.

  16. Study of octupole deformation in n-rich Ba isotopes populated via $\\beta$-decay

    CERN Multimedia

    We propose to exploit the unique capability of the ISOLDE facility to produce $^{150, 151, 152}$Cs beams to investigate their radioactive $\\beta$-decay to $^{150, 151, 152}$Ba. The interest to study this mass region is twofold: these nuclei are expected to show octupole deformations already in their low-lying state, secondly information on the $\\beta$-decay is needed for the nuclear astrophysical model. The experiment will be performed with the ISOLDE Decay Station (IDS) setup using the fast tape station of K.U.-Leuven, equipped with four Clover Germanium detectors, four LaBr$_{3}$(Ce) detectors and one LEP HPGe detector. Information on the $\\beta$-decay, such as lifetimes and delayed neutron-emission probabilities, will be extracted, together with the detailed spectroscopy of the daughter nuclei, via $\\gamma$-$\\gamma$-coincidences and lifetime measurement of specific states.

  17. Resistance training & beta-hydroxy-beta-methylbutyrate supplementation on hormones

    Directory of Open Access Journals (Sweden)

    Hamid Arazi


    Full Text Available RESUMOIntroduction:In recent years, there was an increased interest on the effects of beta-hydroxy-beta-methylbutyrate (HMB supplementation on skeletal muscle due to its anti-catabolic effects.Objectives:To investigate the effect of HMB supplementation on body composition, muscular strength and anabolic-catabolic hormones after resistance training.Methods:Twenty amateur male athletes were randomly assigned to supplement and control groups in a double-blind crossover design and participated in four weeks resistance training. Before and after the test period fasting blood samples were obtained to determine anabolic (the growth hormone and testosterone and catabolic (cortisol hormones, and fat mass, lean body mass (LBM and muscular strength were measured. Dependent and independent t-tests were used to analyze data.Results:After the training period, there were no significant differen-ces between the groups with respect to fat mass, LBM and anabolic-catabolic hormones. HMB supplementation resulted in a significantly greater strength gain (p≤0.05.Conclusion:Greater increase in strength for HMB group was not accompanied by body composition and basal circulating anabolic-catabolic hormonal changes. It seems that HMB supplementation may have beneficial effects on neurological adaptations of strength gain.

  18. Abstraction Mechanisms in the BETA Programming Language

    DEFF Research Database (Denmark)

    Kristensen, Bent Bruun; Madsen, Ole Lehrmann; Møller-Pedersen, Birger


    ]) --- covering both data, procedural and control abstractions, substituting constructs like class, procedure, function and type. Correspondingly objects, procedure activation records and variables are all regarded as special cases of the basic building block of program executions: the entity. A pattern thus......The BETA programming language is developed as part of the BETA project. The purpose of this project is to develop concepts, constructs and tools in the field of programming and programming languages. BETA has been developed from 1975 on and the various stages of the language are documented in [BETA...... a]. The application area of BETA is programming of embedded as well as distributed computing systems. For this reason a major goal has been to develop constructs that may be efficiently implemented. Furthermore the BETA language is intended to have a few number of basic but general constructs...

  19. Point defects behavior in beta Cu-based shape memory alloys

    Energy Technology Data Exchange (ETDEWEB)

    Romero, R.; Somoza, A. [Univ. Nacional del Centro de la Provincia de Buenos Aires, Tandil (Argentina). IFIMAT


    A summary of positron annihilation spectroscopy data relating to the point defect behavior after quenching and to thermal equilibrium in {beta}-phase Cu-based shape memory alloys Cu-Zn-Al and Cu-Al-Be is presented. Particular attention is given to the initial concentration of quenched-in vacancies as a function of the quenching temperature, migration of the retained point defects with aging temperature and time, and the vacancy formation and migration energies. (orig000.

  20. Progress in field spectroscopy

    NARCIS (Netherlands)

    Milton, E.J.; Schaepman, M.E.; Anderson, K.; Kneubühler, M.; Fox, N.


    This paper reviews developments in the science of field spectroscopy, focusing on the last twenty years in particular. During this period field spectroscopy has become established as an important technique for characterising the reflectance of natural surfaces in situ, for supporting the vicarious c

  1. Heterodyned holographic spectroscopy

    NARCIS (Netherlands)

    Douglas, NG


    In holographic spectroscopy an image of an interference pattern is projected onto a detector and transformed back to the input spectrum. The general characteristics are similar to those of Fourier transform spectroscopy, but the spectrum is obtained without scanning. In the heterodyned arrangement o

  2. Metallomic EPR spectroscopy. (United States)

    Hagen, Wilfred R


    Based on explicit definitions of biomolecular EPR spectroscopy and of the metallome, this tutorial review positions EPR in the field of metallomics as a unique method to study native, integrated systems of metallobiomolecular coordination complexes subject to external stimuli. The specific techniques of whole-system bioEPR spectroscopy are described and their historic, recent, and anticipated applications are discussed.

  3. Double-loaded liposomes encapsulating Quercetin and Quercetin beta-cyclodextrin complexes: Preparation, characterization and evaluation

    Directory of Open Access Journals (Sweden)

    Jessy Shaji


    Full Text Available Beta-cyclodextrin (CD inclusion complexes of Quercetin were formed and characterized by Differential scanning calorimetry (DSC and Fourier transform infra-red spectroscopy (FTIR spectroscopy. Plain Quercetin liposomes using phosphatidylcholine and cholesterol were prepared and optimized. Factors such as ratio of lipids employed, drug:lipid ratio, etc. were fine tuned and optimized to achieve maximum entrapment of the Quercetin into the bilayer. Entrapment was further enhanced by double loading the liposomes. These were prepared by incorporating Quercetin as a plain drug as well as the inclusion complexes within the lipid bilayer and the aqueous compartment, respectively, of the liposomes using the thin film hydration technique. The highest entrapment was achieved with a lipid ratio of 9:1, and the amount of plain drug entering the bilayer was 1/10 th the amount of lipid employed. Double loading increased this value to one part of drug per five parts of lipid when Quercetin-beta-CD (1:1 mol/mol was entrapped. The release of Quercetin from liposomes was highest when the drug was entrapped in the form of a complex with beta cylodextrin. The high entrapment ability of Quercetin in the form of plain drug as well as beta cylodextrin-Quercetin complexes in comparison with plain drug is an indubitable advantage of this approach.

  4. Quantum-limit spectroscopy

    CERN Document Server

    Ficek, Zbigniew


    This book covers the main ideas, methods, and recent developments of quantum-limit optical spectroscopy and applications to quantum information, resolution spectroscopy, measurements beyond quantum limits, measurement of decoherence, and entanglement. Quantum-limit spectroscopy lies at the frontier of current experimental and theoretical techniques, and is one of the areas of atomic spectroscopy where the quantization of the field is essential to predict and interpret the existing experimental results. Currently, there is an increasing interest in quantum and precision spectroscopy both theoretically and experimentally, due to significant progress in trapping and cooling of single atoms and ions. This progress allows one to explore in the most intimate detail the ways in which light interacts with atoms and to measure spectral properties and quantum effects with high precision. Moreover, it allows one to perform subtle tests of quantum mechanics on the single atom and single photon scale which were hardly eve...

  5. Stoichiometrically different inclusion complexes of 2-aminofluorene and 2-amino-9-hydroxyfluorene in beta-cyclodextrin: a spectrofluorimetric study. (United States)

    Enoch, I V Muthu Vijayan; Swaminathan, M


    Beta-cyclodextrin (beta-CDx) forms inclusion complexes with 2-aminofluorene (2AF) and 2-amino-9-hydroxyfluorene (2AHF) in different stoichiometries (Guest-host ratio 1:1 and 1:2 respectively) which is discussed on the basis of study by absorption and fluorescence spectroscopy. The ground and the excited state acidity constants for the neutral-monocation equilibrium of the two fluorophores in aqueous beta-CDx medium are determined by spectrophotometric and fluorimetric titration methods respectively. The dual fluorescence observed for 2AHF monocation in aqueous solution is due to the formation of monocation-water exciplex. This monocation-water exciplex formation is hindered in beta-CDx solution by the inclusion complexation. Based on the results obtained, the structures of the inclusion complexes are proposed.

  6. Milk Intolerance, Beta-Casein and Lactose. (United States)

    Pal, Sebely; Woodford, Keith; Kukuljan, Sonja; Ho, Suleen


    True lactose intolerance (symptoms stemming from lactose malabsorption) is less common than is widely perceived, and should be viewed as just one potential cause of cows' milk intolerance. There is increasing evidence that A1 beta-casein, a protein produced by a major proportion of European-origin cattle but not purebred Asian or African cattle, is also associated with cows' milk intolerance. In humans, digestion of bovine A1 beta-casein, but not the alternative A2 beta-casein, releases beta-casomorphin-7, which activates μ-opioid receptors expressed throughout the gastrointestinal tract and body. Studies in rodents show that milk containing A1 beta-casein significantly increases gastrointestinal transit time, production of dipeptidyl peptidase-4 and the inflammatory marker myeloperoxidase compared with milk containing A2 beta-casein. Co-administration of the opioid receptor antagonist naloxone blocks the myeloperoxidase and gastrointestinal motility effects, indicating opioid signaling pathway involvement. In humans, a double-blind, randomized cross-over study showed that participants consuming A1 beta-casein type cows' milk experienced statistically significantly higher Bristol stool values compared with those receiving A2 beta-casein milk. Additionally, a statistically significant positive association between abdominal pain and stool consistency was observed when participants consumed the A1 but not the A2 diet. Further studies of the role of A1 beta-casein in milk intolerance are needed.

  7. Sawtooth crashes at high beta on JET

    Energy Technology Data Exchange (ETDEWEB)

    Alper, B.; Huysmans, G.T.A.; Sips, A.C.C. [Commission of the European Communities, Abingdon (United Kingdom). JET Joint Undertaking; Nave, M.F.F. [Universidade Tecnica, Lisbon (Portugal). Inst. Superior Tecnico


    The sawtooth crashes on JET display features which depend on beta. The main observation is a transient bulging of flux surfaces (duration inferior to 30 microsec.), which is predominantly on the low field side and extends to larger radii as beta increases. This phenomenon reaches the plasma boundary when beta{sub N} exceeds 0.5 and in these cases is followed by an ELM within 50 microsec. These sawtooth/ELM events limit plasma performance. Modelling of mode coupling shows qualitative agreement between observations of the structure of the sawtooth precursor and the calculated internal kink mode at high beta. (authors). 6 refs., 5 figs.

  8. Milk Intolerance, Beta-Casein and Lactose

    Directory of Open Access Journals (Sweden)

    Sebely Pal


    Full Text Available True lactose intolerance (symptoms stemming from lactose malabsorption is less common than is widely perceived, and should be viewed as just one potential cause of cows’ milk intolerance. There is increasing evidence that A1 beta-casein, a protein produced by a major proportion of European-origin cattle but not purebred Asian or African cattle, is also associated with cows’ milk intolerance. In humans, digestion of bovine A1 beta-casein, but not the alternative A2 beta-casein, releases beta-casomorphin-7, which activates μ-opioid receptors expressed throughout the gastrointestinal tract and body. Studies in rodents show that milk containing A1 beta-casein significantly increases gastrointestinal transit time, production of dipeptidyl peptidase-4 and the inflammatory marker myeloperoxidase compared with milk containing A2 beta-casein. Co-administration of the opioid receptor antagonist naloxone blocks the myeloperoxidase and gastrointestinal motility effects, indicating opioid signaling pathway involvement. In humans, a double-blind, randomized cross-over study showed that participants consuming A1 beta-casein type cows’ milk experienced statistically significantly higher Bristol stool values compared with those receiving A2 beta-casein milk. Additionally, a statistically significant positive association between abdominal pain and stool consistency was observed when participants consumed the A1 but not the A2 diet. Further studies of the role of A1 beta-casein in milk intolerance are needed.

  9. Challenges in Double Beta Decay

    Directory of Open Access Journals (Sweden)

    Oliviero Cremonesi


    Full Text Available In the past ten years, neutrino oscillation experiments have provided the incontrovertible evidence that neutrinos mix and have finite masses. These results represent the strongest demonstration that the electroweak Standard Model is incomplete and that new Physics beyond it must exist. In this scenario, a unique role is played by the Neutrinoless Double Beta Decay searches which can probe lepton number conservation and investigate the Dirac/Majorana nature of the neutrinos and their absolute mass scale (hierarchy problem with unprecedented sensitivity. Today Neutrinoless Double Beta Decay faces a new era where large-scale experiments with a sensitivity approaching the so-called degenerate-hierarchy region are nearly ready to start and where the challenge for the next future is the construction of detectors characterized by a tonne-scale size and an incredibly low background. A number of new proposed projects took up this challenge. These are based either on large expansions of the present experiments or on new ideas to improve the technical performance and/or reduce the background contributions. In this paper, a review of the most relevant ongoing experiments is given. The most relevant parameters contributing to the experimental sensitivity are discussed and a critical comparison of the future projects is proposed.

  10. Fourier transform Raman spectroscopy of the four crystallographic phases of {alpha}, {beta}, {gamma} and {epsilon} 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazatetracyclo[{sup 5,9}.0{sup 3,11}]dodecane (HNIW, CL-20)

    Energy Technology Data Exchange (ETDEWEB)

    Goede, Patrick; Latypov, Nikolaj V.; Oestmark, Henric [Department of Energetic Materials, Grindsjoen Research Center, Swedish Defence Research Agency, FOI, SE-147 25 Tumba (Sweden)


    Fourier transform Raman Spectroscopy (Nd: YAG laser at 1064 nm) was used to characterize the four stable phases of 2,4,6,8,10,12-Hexanitro-2,4,6,8,10,12-hexaazatetracyclo[{sup 5,9}.0{sup 3,11}]dodecane (HNIW, CL-20). Raman spectra are reported over the region from 0-4000 cm{sup -1}{sub ,} relative to the laser line. A tentative assignment of the most predominant Raman peaks was made with the aid of QM calculations at the B3LYP/6-31G(d) level. A method for detecting polymorphic impurities in {epsilon}-CL-20 was also developed. The detection level for polymorphic impurities was determined to be below 2%. A method for producing {gamma}-CL-20 is also presented. (Abstract Copyright [2004], Wiley Periodicals, Inc.)

  11. The pharmacokinetics of beta-cyclodextrin and hydroxypropyl-beta-cyclodextrin in the rat

    NARCIS (Netherlands)

    Frijlink, H W; Visser, J; Hefting, N R; Oosting, R; Meijer, D K; Lerk, C F


    Hydroxypropyl-beta-cyclodextrin was analyzed by HPLC using postcolumn complexation with phenolphthalein and negative colorimetric detection, with a detection limit of 20 micrograms/ml. The pharmacokinetics of beta-cyclodextrin and of hydroxypropyl-beta-cyclodextrin were studied after intravenous adm

  12. The impact of beta-elemene on beta-tubulin of human hepatoma hepg2 cells

    Institute of Scientific and Technical Information of China (English)

    Yuqiu Mao; Liying Ban; Jielin Zhang; Li Hou; Xiaonan Cui


    Objective:The aim of this study was to investigate the impact of beta-elemene injection on the growth and beta-tubulin of human hepatocarcinoma HepG2 cells. Methods:cellproliferation was assessed by MTT assay. cellcycle distribution was detected by flow cytometry (FCM). The mRNA expression of beta-tubulin was measured by RT-PCR. West-ern blot analysis was used to determine protein expression of beta-tubulin and the polymerization of beta-tubulin. Results:Beta-elemene injection inhibited HepG2 cells proliferation in a dose-and time-dependent manner;FCM analysis indicated beta-elemene injection induced cellcycle arrested at S phase. RT-PCR and western-blot analysis showed that beta-elemene injection down-regulated beta-tubulin expression at both mRNA and protein levels, presenting a dose-dependent manner. Moreover, beta-elemene injection reduced the polymerization of microtubules in a dose-dependent manner. Conclusion:Beta-elemene injection can inhibit the proliferation of hepatoma HepG2 cells, the mechanism might be partly related to the down-regulation of beta-tubulin and inhibition of microtubular polymerization.

  13. Expressions of GSK-3beta, Beta-Catenin and PPAR-Gamma in Medulloblastoma

    Institute of Scientific and Technical Information of China (English)

    Xiong Zhang; Lu Si; Yu Li; Can Mi


    Objective: To investigate the expressions of GSK-3beta, Beta-catenin and PPAR-gamma, and their relationship in medulloblastoma, and to explore their value in clinic application.Methods: Immunohistochemical staining with SP method was conducted to determine the expressions of GSK-3beta, Beta-catenin and PPAR-gamma in 48 cases of medulloblastoma and 10 normal cerebellar tissues.Results: The rate of abnormal expressions of beta-catenin and PPAR-gamma in MB was higher than that in normal. Conversely, GSK-3beta in MB was lower than that in the normal (P<0.05). Furthermore, in medulloblastoma, beta-catenin and GSK-3beta showed a negative correlation, PPAR-gamma and beta-catenin had a positive correlation.Conclusion: Abnormal expression of beta-catenin plays a crucial role in the development of medulloblastoma. Meanwhile, PPAR-gamma and GSK-3beta which are tightly related with beta-catenin are both involved in the genesis and development of medulloblastoma.

  14. Imperfect World of beta beta-decay Nuclear Data Sets

    Energy Technology Data Exchange (ETDEWEB)

    Pritychenko, B. [Brookhaven National Lab. (BNL), Upton, NY (United States). NNDC


    The precision of double-beta ββ-decay experimental half lives and their uncertainties is reanalyzed. The method of Benford's distributions has been applied to nuclear reaction, structure and decay data sets. First-digit distribution trend for ββ-decay T2v1/2 is consistent with large nuclear reaction and structure data sets and provides validation of experimental half-lives. A complementary analysis of the decay uncertainties indicates deficiencies due to small size of statistical samples, and incomplete collection of experimental information. Further experimental and theoretical efforts would lead toward more precise values of-decay half-lives and nuclear matrix elements.

  15. Beta-glucosidase I variants with improved properties

    Energy Technology Data Exchange (ETDEWEB)

    Bott, Richard R.; Kaper, Thijs; Kelemen, Bradley; Goedegebuur, Frits; Hommes, Ronaldus Wilhelmus; Kralj, Slavko; Kruithof, Paulien; Nikolaev, Igor; Van Der Kley, Wilhelmus Antonious Hendricus; Van Lieshout, Johannes Franciscus Thomas; Van Stigt Thans, Sander


    The present disclosure is generally directed to enzymes and in particular beta-glucosidase variants. Also described are nucleic acids encoding beta-glucosidase variants, compositions comprising beta-glucosidase variants, methods of using beta-glucosidase variants, and methods of identifying additional useful beta-glucosidase variants.

  16. Dosimetry of low-energy beta radiation

    Energy Technology Data Exchange (ETDEWEB)

    Borg, J.


    Useful techniques and procedures for determination of absorbed doses from exposure in a low-energy {beta} radiation field were studied and evaluated in this project. The four different techniques included were {beta} spectrometry, extrapolation chamber dosimetry, Monte Carlo (MC) calculations, and exoelectron dosimetry. As a typical low-energy {beta} radiation field a moderated spectrum from a {sup 14}C source (E{sub {beta}},{sub max} =156 keV) was chosen for the study. The measured response of a Si(Li) detector to photons (bremsstrahlung) showed fine agreement with the MC calculated photon response, whereas the difference between measured and MC calculated responses to electrons indicates an additional dead layer thickness of about 12 {mu}m in the Si(Li) detector. The depth-dose profiles measured with extrapolation chambers at two laboratories agreed very well, and it was confirmed that the fitting procedure previously reported for {sup 147}Pm depth-dose profiles is also suitable for {beta} radiation from {sup 14}C. An increasing difference between measured and MC calculated dose rates for increasing absorber thickness was found, which is explained by limitations of the EGS4 code for transport of very low-energy electrons (below 10-20 keV). Finally a study of the thermally stimulated exoelectron emission (TSEE) response of BeO thin film dosemeters to {beta} radiation for radiation fields with maximum {beta} energies ranging from 67 keV to 2.27 MeV is reported. For maximum {beta} energies below approximately 500 keV, a decrease in the response amounting to about 20% was observed. It is thus concluded that a {beta} dose higher than about 10 {mu}Gy can be measured with these dosemeters to within 0 to -20% independently of the {beta}energy for E{sub {beta}},{sub max} values down to 67 keV. (au) 12 tabs., 38 ills., 71 refs.

  17. Narrow Bandgap in beta-BaZn2As2 and Its Chemical Origins

    CERN Document Server

    Xiao, Zewen; Ueda, Shigenori; Toda, Yoshitake; Ran, Fan-Yong; Guo, Jiangang; Lei, Hechang; Matsuishi, Satoru; Hosono, Hideo; Kamiya, Toshio


    Beta-BaZn2As2 is known to be a p-type semiconductor with the layered crystal structure similar to that of LaZnAsO, leading to the expectation that beta-BaZn2As2 and LaZnAsO have similar bandgaps; however, the bandgap of beta-BaZn2As2 (previously-reported value ~0.2 eV) is one order of magnitude smaller than that of LaZnAsO (1.5 eV). In this paper, the reliable bandgap value of beta-BaZn2As2 is determined to be 0.23 eV from the intrinsic region of the tem-perature dependence of electrical conductivity. The origins of this narrow bandgap are discussed based on the chemi-cal bonding nature probed by 6 keV hard X-ray photoemission spectroscopy, hybrid density functional calculations, and the ligand theory. One origin is the direct As-As hybridization between adjacent [ZnAs] layers, which leads to a secondary splitting of As 4p levels and raises the valence band maximum. The other is that the non-bonding Ba 5dx2-y2 orbitals form unexpectedly deep conduction band minimum (CBM) in beta-BaZn2As2 although the CBM of L...

  18. Cavity-enhanced spectroscopies

    CERN Document Server

    van Zee, Roger


    ""Cavity-Enhanced Spectroscopy"" discusses the use of optical resonators and lasers to make sensitive spectroscopic measurements. This volume is written by the researcchers who pioneered these methods. The book reviews both the theory and practice behind these spectroscopic tools and discusses the scientific discoveries uncovered by these techniques. It begins with a chapter on the use of optical resonators for frequency stabilization of lasers, which is followed by in-depth chapters discussing cavity ring-down spectroscopy, frequency-modulated, cavity-enhanced spectroscopy, intracavity spectr

  19. Infrared and Raman characterization of beta iron silicide (United States)

    Lefki, K.; Muret, P.; Bustarret, E.; Boutarek, N.; Madar, R.; Chevrier, J.; Derrien, J.; Brunel, M.


    Samples of beta-iron silicide were prepared by three different methods : solid phase reaction on silicon (111), on a monocrystaline FeSi substrate, and from the melt. These samples have been characterized by x-ray diffraction and investigated by Infrared and Raman spectroscopies. The infrared and Raman lines are compared with theoretical predictions given by the factor group analysis of the silicide primitive cell, which yields the number and the symmetry of the different modes. We relate the red shift of the Infrared and Raman lines on samples with smaller lattice parameters to the presence of Iron vacancies in films deposited on silicon, in agreement with the sign of the thermoelectric power.

  20. Radiopurity control in the NEXT-100 double beta decay experiment

    Energy Technology Data Exchange (ETDEWEB)

    Álvarez, V.; Cárcel, S.; Cervera, A.; Díaz, J.; Ferrario, P.; Gil, A.; Gómez-Cadenas, J. J.; Laing, A.; Liubarsky, I.; Lorca, D.; Martín-Albo, J.; Martínez, A.; Monrabal, F.; Muñoz Vidal, J.; Nebot-Guinot, M.; Rodríguez, J.; Serra, L.; Simón, A.; Sofka, C.; Sorel, M. [Instituto de Física Corpuscular (IFIC), CSIC and Universitat de València, 46980 Paterna, Valencia (Spain); and others


    An extensive material screening and selection process is underway in the construction of the 'Neutrino Experiment with a Xenon TPC' (NEXT), intended to investigate neutrinoless double beta decay using a high-pressure xenon gas TPC filled with 100 kg of Xe enriched in {sup 136}Xe. Determination of the radiopurity levels of the materials is based on gamma-ray spectroscopy using ultra-low background germanium detectors at the Laboratorio Subterráneo de Canfranc (Spain) and also on Glow Discharge Mass Spectrometry. Materials to be used in the shielding, pressure vessel, electroluminescence and high voltage components and energy and tracking readout planes have been already taken into consideration. The measurements carried out are presented, describing the techniques and equipment used, and the results obtained are shown, discussing their implications for the NEXT experiment.

  1. Anticorrosion Nanocrystalline Beta Zeolite Thin Film for Advanced Applications

    Directory of Open Access Journals (Sweden)

    Maha Saud M. Al-subaie


    Full Text Available Steel alloys corrosion is ubiquitous and is conventionally protected by anticorrosion chromate coatings. However, the process suffers from the release of carcinogenic hexavalent chromium ions that needs to be replaced by an ecofriendly alternative. In this context, the need for the development of satisfactory ecofriendly chromium-free coating with superior corrosion performance is highly desirable. In the present study, we synthesized fully dispersible nanocrystalline Beta zeolite seeds and coated on steel alloys followed by steaming. The samples were characterized by XRD, FE-SEM, and DLS analyses. The anticorrosion behavior of the synthesized nanoparticle coatings on steel alloys was investigated by electrochemical measurements (DC polarization and electrochemical impedance spectroscopy (EIS in NaCl and acid and alkaline media under identical experimental conditions. The present study demonstrated that the nanozeolite coating can be a potential alternative for toxic and carcinogenic chromate coating.

  2. Radiopurity control in the NEXT-100 double beta decay experiment (United States)

    Álvarez, V.; Bandac, I.; Bettini, A.; Borges, F. I. G. M.; Cárcel, S.; Castel, J.; Cebrián, S.; Cervera, A.; Conde, C. A. N.; Dafni, T.; Dias, T. H. V. T.; Díaz, J.; Egorov, M.; Esteve, R.; Evtoukhovitch, P.; Fernandes, L. M. P.; Ferrario, P.; Ferreira, A. L.; Freitas, E. D. C.; Gehman, V. M.; Gil, A.; Goldschmidt, A.; Gómez, H.; Gómez-Cadenas, J. J.; González-Díaz, D.; Gutiérrez, R. M.; Hauptman, J.; Hernando Morata, J. A.; Herrera, D. C.; Iguaz, F. J.; Irastorza, I. G.; Jinete, M. A.; Labarga, L.; Laing, A.; Liubarsky, I.; Lopes, J. A. M.; Lorca, D.; Losada, M.; Luzón, G.; Marí, A.; Martín-Albo, J.; Martínez, A.; Miller, T.; Moiseenko, A.; Monrabal, F.; Monteiro, C. M. B.; Mora, F. J.; Moutinho, L. M.; Muñoz Vidal, J.; da Luz, H. Natal; Navarro, G.; Nebot-Guinot, M.; Nygren, D.; Oliveira, C. A. B.; de Solórzano, A. Ortiz; Palma, R.; Pérez, J.; Pérez Aparicio, J. L.; Renner, J.; Ripoll, L.; Rodríguez, A.; Rodríguez, J.; Santos, F. P.; dos Santos, J. M. F.; Segui, L.; Serra, L.; Shuman, D.; Simón, A.; Sofka, C.; Sorel, M.; Toledo, J. F.; Tomás, A.; Torrent, J.; Tsamalaidze, Z.; Vázquez, D.; Veloso, J. F. C. A.; Villar, J. A.; Webb, R. C.; White, J. T.; Yahlali, N.


    An extensive material screening and selection process is underway in the construction of the "Neutrino Experiment with a Xenon TPC" (NEXT), intended to investigate neutrinoless double beta decay using a high-pressure xenon gas TPC filled with 100 kg of Xe enriched in 136Xe. Determination of the radiopurity levels of the materials is based on gamma-ray spectroscopy using ultra-low background germanium detectors at the Laboratorio Subterráneo de Canfranc (Spain) and also on Glow Discharge Mass Spectrometry. Materials to be used in the shielding, pressure vessel, electroluminescence and high voltage components and energy and tracking readout planes have been already taken into consideration. The measurements carried out are presented, describing the techniques and equipment used, and the results obtained are shown, discussing their implications for the NEXT experiment.

  3. Inclusion complex of butachlor with beta-cyclodextrin: characterization, solubility, and speciation-dependent adsorption. (United States)

    Bian, Haitao; Chen, Jingwen; Cai, Xiyun; Liu, Ping; Liu, Huihui; Qiao, Xianliang; Huang, Liping


    Due to soil adsorption, higher amounts of the herbicide butachlor are necessary to achieve its herbicidal activity, hence increasing its environmental risks. In this study, the effects of beta-cyclodextrin (beta-CD) on solubility and soil adsorption of butachlor were investigated. Formation of a 1:1 stoichiometric inclusion complex between them with an apparent stability constant of 443 L mol(-1) was confirmed in the solution. Fourier transform infrared spectroscopy showed that the (N-CO) amide bond and alkyl ether moiety of butachlor molecule could enter into the cavity of beta-CD, but the double-substituted aromatic ring was excluded because it was larger size than the cavity. Significant enhancing dissolution of butachlor in the inclusion complex occurred in comparison to the free herbicide. The adsorption of butachlor on soil was reduced with an increase of beta-CD concentration because of the formation of the inclusion complex with low adsorption potency. Although the sorption distribution coefficient of complexed butachlor (i.e., butachlor/beta-cyclodextrin inclusion complex) (K(d,c) = 6.14) was about 14% of that of the free herbicide (K(d,f) = 44.54), the proportion of the adsorbed amount of complexed butachlor to the total adsorbed amount rose with the increase of beta-CD concentration. Thus, the adsorption of inclusion complex cannot be neglected in the presence of high concentrations cyclodextrins, although its water solubility was much higher than that of the free herbicide. These results indicate that beta-CD may be used as a formation additive to improve the solubility of butachlor, reduce its adsorption on soil, and increase the availability of butachlor for weeds.

  4. Signaling from beta1- and beta2-adrenergic receptors is defined by differential interactions with PDE4

    DEFF Research Database (Denmark)

    Richter, Wito; Day, Peter; Agrawal, Rani


    Beta1- and beta2-adrenergic receptors (betaARs) are highly homologous, yet they play clearly distinct roles in cardiac physiology and pathology. Myocyte contraction, for instance, is readily stimulated by beta1AR but not beta2AR signaling, and chronic stimulation of the two receptors has opposing...

  5. Localization of thymosin beta-4 in tumors

    DEFF Research Database (Denmark)

    Larsson, L. -I.; Holck, Susanne


    Overexpression of thymosin beta-4 has been linked to malignant progression but the localization of this polypeptide within tumors is incompletely known. We therefore examined breast cancers for thymosin beta-4 using immunofluorescence. Reactive cells were identified with monoclonal cell marker...... in the tumor microenvironment may modulate tumor behavior....

  6. Nebivolol : third-generation beta-blockade

    NARCIS (Netherlands)

    de Boer, Rudolf A.; Voors, Adriaan A.; van Veldhuisen, Dirk J.


    Nebivolol is a third generation beta-blocker. It is highly selective for the beta 1-adrenoceptor, and has additional nitric oxide-mediated vasodilating and antioxidant properties, along with a favourable metabolic profile. Nebivolol is well tolerated by patients with hypertension and heart failure.

  7. The beta subunit of casein kinase II

    DEFF Research Database (Denmark)

    Boldyreff, B; Piontek, K; Schmidt-Spaniol, I;


    cDNAs encoding the beta subunit of pig and mouse CKII were isolated. The porcine cDNA was expressed as a fusion protein in Escherichia coli and used for the production of anti-CKII-beta subunit specific antibodies....

  8. Venus: Geology of Beta Regio rift system (United States)

    Nikishin, A. M.; Borozdin, V. K.; Bobina, N. N.


    Beta Regio is characterized by the existence of rift structures. We compiled new geologic maps of Beta Regio according to Magellan data. There are many large uplifted tesserae on beta upland. These tesserae are partly buried by younger volcanic cover. We can conclude, using these observations, that Beta upland formed mainly due to lithospheric tectonic uplifting and was only partly constructed by volcanism. Theia Mons is the center of the Beta rift system. Many rift belts are distributed radially to Theia Mons. Typical widths of rifts are 40-160 km. Rift valleys are structurally represented by crustal grabens or half-grabens. There are symmetrical and asymmetrical rifts. Many rifts have shoulder uplifts up to 0.5-1 km high and 40-60 km wide. Preliminary analysis for rift valley structural cross sections lead to the conclusion that rifts originated due to 5-10 percent crustal extension. Many rifts traverse Beta upland and spread to the surrounding lowlands. We can assume because of these data that Beta rift system has an active-passive origin. It formed due to regional tectonic lithospheric extension. Rifting was accelerated by upper-mantle hot spot origination under the center of passive extension (under the Beta Regio).

  9. Beta-agonists and animal welfare (United States)

    The use of beta-agonists in animal feed is a high profile topic within the U.S. as consumers and activist groups continue to question its safety. The only beta-agonist currently available for use in swine is ractopamine hydrochloride (RAC). This is available as Paylean™ (Elanco Animal Health – FDA a...

  10. Shielding for beta-gamma radiation. (United States)

    Fletcher, J J


    The build-up factor, B, for lead was expressed as a polynominal cubic function of the relaxation length, mu x, and incorporated in a "general beta-gamma shielding equation." A computer program was written to determine shielding thickness for polyenergetic beta-gamma sources without resorting to the conventional "add-one-HVL" method.

  11. Preparation of Black Hoof medicinal mushroom Phellinus linteus (Berk. et M.A. Curt.) Teng (Aphyllophoromycetideae) beta-glucan sulfate and in vitro tumor cell growth inhibitory activity. (United States)

    Bae, In Young; Shin, Ji-Yoon; Lee, Hyeon Gyu


    Polysaccharide beta-glucans were extracted from the medicinal mushroom Phellinus linteus (Hymenochaetaceae, Aphyllophoromycetideae) and subjected to sulfation. Chemical modification of the beta-glucan was confirmed by structural analysis, and its biological properties were compared with those of native beta-glucan. The results of Fourier transform infrared spectroscopy and elemental analysis indicated that successive preparation of the sulfated derivative yielded a degree of substitution of 0.47. Nitric oxide production measured by the bronchoalveolar lavage (BAL) experiments increased 1.5-fold after sulfation. In addition, the introduction of sulfate groups into the beta-glucan chains improved in vitro growth inhibitory activity against SNU-C2A cells. Therefore, sulfated beta-glucan extracted from Ph. linteus may be beneficial for immune support due to its incorporation of functional groups into its polymer structure.

  12. Identification of a novel angiotensin-I-converting enzyme inhibitory peptide corresponding to a tryptic fragment of bovine beta-lactoglobulin. (United States)

    Mullally, M M; Meisel, H; FitzGerald, R J


    The angiotensin-I-converting enzyme (ACE) inhibitory activity of a tryptic digest of bovine beta-lactoglobulin (beta-lg) was investigated. Intact beta-lg essentially did not inhibit ACE while the tryptic digest gave an 84.3% inhibition of ACE. Peptide material eluting between 20 and 25% acetonitrile during C18 solid-phase extraction of the beta-lg tryptic digest inhibited ACE by 93.6%. This solid-phase extraction fraction was shown by mass spectroscopy to contain beta-lg f(142-148). This peptide had an ACE IC50 value of 42.6 micromol/l. The peptide was resistant to further digestion with pepsin and was hydrolysed to a very low extent with chymotrypsin. The contribution of specific amino acid residues within the peptide to ACE inhibitory activity and the potential application of this peptide as a nutraceutical is discussed.

  13. [Degradation of beta-naphthol by catalytic wet air oxidation]. (United States)

    Liu, Jie; Yu, Chao-Ying; Zhao, Pei-Qing; Chen, Ge-Xin


    A series of MnO(x)/nano-TiO2 catalysts were prepared and their application in degradation of beta-naphthol by catalytic wet air oxidation (CWAO) was investigated. The catalysts preparation conditions, reaction conditions and its stability were tested. The catalysts had been characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and temperature-programmed reduction (TPR) measurements. The results showed that the decrease of the COD removal for the degradation of beta-naphthol at high Mn loading was due to the aggregation of the highly dispersed Mn species and the formation of the correlated crystals. The decline of the COD removal at high calcination temperature was probably attributed to the weak electron transfer between Mn2O3 and MnO2 and the formation of the inactive Mn2O3. The COD removal had been falling slightly when the catalyst was used 6 times, and this was likely related to the decrease of the diffraction peaks. The catalyst had a high activity when the Mn loading (mass fraction) was 4% and the calcination temperature was 450 degrees C. The COD removal was up to 96.4% at 110 degrees C and 0.5 MPa with this catalyst. The COD removal of 92.4% could be obtained with the MnO(x)/nano-TiO2 catalyst was recycled 6 times. The Mn leaching at 50, 80, 110 and 150 degrees C were all less than 9.3 mg x L(-1) by means of Atomic Absorption Spectroscopy (AAS). The probable degradation pathway was proposed according to some publications.

  14. Fluorescence correlation spectroscopy

    NARCIS (Netherlands)

    Hink, M.A.; Verveer, P.J.


    Fluorescence fluctuation spectroscopy techniques allow the quantification of fluorescent molecules present at the nanomolar concentration level. After a brief introduction to the technique, this chapter presents a protocol including background information in order to measure and quantify the molecul

  15. Hadron Spectroscopy -- Theory

    CERN Document Server

    Swanson, E S


    A brief review of theoretical progress in hadron spectroscopy and nonperturbative QCD is presented. Attention is focussed on recent lattice gauge theory, the Dyson-Schwinger formalism, unquenching constituent models, and some beyond the Standard Model physics.

  16. X-ray scattering signatures of {beta}-thalassemia

    Energy Technology Data Exchange (ETDEWEB)

    Desouky, Omar S. [Radiation Physics Department, National Center for Radiation Research and Technology (NCRRT) (Egypt); Elshemey, Wael M. [Biophysics Department, Faculty of Science, Cairo University (Egypt)], E-mail:; Selim, Nabila S. [Radiation Physics Department, National Center for Radiation Research and Technology (NCRRT) (Egypt)


    X-ray scattering from lyophilized proteins or protein-rich samples is characterized by the presence of two characteristic broad peaks at scattering angles equivalent to momentum transfer values of 0.27 and 0.6 nm{sup -1}, respectively. These peaks arise from the interference of coherently scattered photons. Once the conformation of a protein is changed, these two peaks reflect such change with considerable sensitivity. The present work examines the possibility of characterizing the most common cause of hemolytic anaemia in Egypt and many Mediterranean countries; {beta}-thalassemia, from its X-ray scattering profile. This disease emerges from a genetic defect causing reduced rate in the synthesis of one of the globin chains that make up hemoglobin. As a result, structurally abnormal hemoglobin molecules are formed. In order to detect such molecular disorder, hemoglobin samples of {beta}-thalassemia patients are collected, lyophilized and measured using a conventional X-ray diffractometer. Results show significant differences in the X-ray scattering profiles of most of the diseased samples compared to control. The shape of the first scattering peak at 0.27 nm{sup -1}, in addition to the relative intensity of the first to the second scattering peaks, provides the most reliable signs of abnormality in diseased samples. The results are interpreted and confirmed with the aid of Fourier Transform Infrared (FTIR) spectroscopy of normal and thalassemia samples.

  17. Biodegradable star polymers functionalized with beta-cyclodextrin inclusion complexes. (United States)

    Setijadi, Eki; Tao, Lei; Liu, Jingquan; Jia, Zhongfan; Boyer, Cyrille; Davis, Thomas P


    Three-armed biodegradable star polymers made from polystyrene (polySt) and poly (polyethylene glycol) acrylate (polyPEG-A) were synthesized via a "core first" methodology using a trifunctional RAFT agent, created by attaching RAFT agents to a core via their R-groups. The resultant three-armed polymeric structures were well-defined, with polydispersity indices less than 1.2. Upon aminolysis and further reaction with dithiodipyridine (DTDP), these three-armed polymers could be tailored with sulfhydryl and pyridyldisulfide (PDS) end functionalities, available for further reaction with any free-sulfhydryl group containing precursors to form disulfide linkages. Nuclear magnetic resonance (NMR) confirmed that more than 98% of the polymer arms retained integral trithiocarbonate active sites after polymerization. Intradisulfide linkages between the core and the arms conferred biodegradability on the star architectures. Subsequently, the arm-termini were attached to cholesterol also via disulfide linkages. The cholesterol terminated arms were then used to form supramolecular structures via inclusion complex formation with beta-cyclodextrin (beta-CD). The star architectures were found to degrade rapidly on treatment with DL-dithiothereitol (DTT). The star polymers and supramolecular structures were characterized using gel permation chromatography (GPC), static light scattering (SLS), 2D NMR, and fluorescence spectroscopy.

  18. VLT imaging of the {\\beta} Pictoris gas disk

    CERN Document Server

    Nilsson, R; Olofsson, G; Fathi, K; Thébault, Ph; Liseau, R


    Circumstellar debris disks older than a few Myr should be largely devoid of primordial gas remaining from the protoplanetary disk phase. Tracing the origin of observed atomic gas in Keplerian rotation in the edge-on debris disk surrounding the ~12 Myr old star {\\beta} Pictoris requires more detailed information about its spatial distribution than has previously been acquired by limited slit spectroscopy. Especially indications of asymmetries and presence of Ca II gas at high disk latitudes call for additional investigation. We set out to recover a complete image of the Fe I and Ca II gas emission around {\\beta} Pic by spatially resolved, high-resolution spectroscopic observations to better understand the morphology and origin of the gaseous disk component. The multiple fiber facility FLAMES/GIRAFFE at the VLT, with the large IFU ARGUS, was used to obtain spatially resolved optical spectra in four regions covering the northeast and southwest side of the disk. Emission lines from Fe I and Ca II were mapped and ...

  19. Beta-glucuronidase in physiology and disease. (United States)

    Basińska, Agnieszka; Floriańczyk, Bolesław


    beta-glucoronidase (EC is a lysosomal enzyme catylysing the decomposition of beta-D-glucoronides--compounds arising as a result of the combination of beta-D-glucoronic acid and a number of compounds both exo- and endogenous, containing hydroxylic, carboxylic, amine, imine or thiol groups. The most common test evaluating the activity of the enzyme is that using phenolphtalein glucoronide as a biosynthetic substrate. The freed aglycons are colorimetrically assayed. The activity of beta-glucoronidase increases in many pathological conditions: liver infammations, cirrhosis of the liver, inflammations of other organs, cholestatic jaundice, tuberculosis, sarcoidosis and also in neoplasms. Many authors point to beta-glucoronidase as a sensitive indicator signalling cell damage.

  20. Precision measurements in 20F beta decay (United States)

    Hughes, Maximilian; Naviliat-Cuncic, Oscar; Voytas, Paul; George, Elizabeth; Paulauskas, Stan; Huyan, Xueying


    Precision measurements of the shape of the beta particle energy spectrum provide a sensitive window to search for new interactions beyond the standard model. The decay of 20F offers an attractive system due to the simple decay scheme for a coincidence measurement. A beam of 20F ions, produced at the National Superconducting Cyclotron Laboratory, was implanted into a beta-detector. A gamma-ray detection system surrounded the beta detector to detect the beta-delayed gammas in coincidence to reduce the background. Preliminary analysis of these data focus on the half-life of 20F due to the statistical inconsistency of previous work. Monte Carlo simulations are ongoing to analyze the shape of the beta energy spectrum. Results of the analysis of the half-life will be presented. Supported by National Science Foundation Grant PHY-1102511.

  1. Ranking Beta Sheet Topologies of Proteins

    DEFF Research Database (Denmark)

    Fonseca, Rasmus; Helles, Glennie; Winter, Pawel


    One of the challenges of protein structure prediction is to identify long-range interactions between amino acids.  To reliably predict such interactions, we enumerate, score and rank all beta-topologies (partitions of beta-strands into sheets, orderings of strands within sheets and orientations...... of paired strands) of a given protein.  We show that the beta-topology corresponding to the native structure is, with high probability, among the top-ranked. Since full enumeration is very time-consuming, we also suggest a method to deal with proteins with many beta-strands. The results reported...... in this paper are highly relevant for ab initio protein structure prediction methods based on decoy generation. The top-ranked beta-topologies can be used to find initial conformations from which conformational searches can be started. They can also be used to filter decoys by removing those with poorly...

  2. The search for neutrinoless double beta decay

    CERN Document Server

    Gomez-Cadenas, J J; Mezzetto, M; Monrabal, F; Sorel, M


    In the last few years the search for neutrinoless double beta decay has evolved from being almost a marginal activity in neutrino physics to one of the highest priorities for understanding neutrinos and the origin of mass. There are two main reasons for this paradigm shift: the discovery of neutrino oscillations, which clearly established the existence of massive neutrinos; and the existence of an unconfirmed, but not refuted, claim of evidence for neutrinoless double decay in 76Ge. As a consequence, a new generation of experiments, employing different detection techniques and {\\beta}{\\beta} isotopes, is being actively promoted by experimental groups across the world. In addition, nuclear theorists are making remarkable progress in the calculation of the neutrinoless double beta decay nuclear matrix elements, thus eliminating a substantial part of the theoretical uncertainties affecting the particle physics interpretation of this process. In this report, we review the main aspects of the double beta decay pro...

  3. Experimental beta-alaninuria induced by (aminooxyacetate.

    Directory of Open Access Journals (Sweden)

    Kurozumi Y


    Full Text Available Experimental beta-alaninuria was induced in rats by injection of (aminooxyacetate (AOA, a potent inhibitor of aminotransferases, in order to elucidate the pathogenesis of hyper-beta-alaninemia. A 27-fold increase of beta-alanine (BALA excretion was induced by subcutaneous injection of 1 5 mg of AOA per kg of body weight. A 13-fold and a 9-fold increase of beta-aminoisobutyric acid (BAIBA and gamma-aminobutyric acid (GABA, respectively, were also induced simultaneously by the AOA injection. Identification of BALA and BAIBA isolated from the rat urine was performed by chromatographic and mass spectrometric analyses. The effects of AOA injection on the tissue levels of these amino acids were also studied. Contents of BALA in the liver and kidney and GABA in the brain increased significantly in response to AOA injection. The present study indicates that BALA transaminase is involved in hyper-beta-alaninemia.

  4. Experimental and theoretical studies on the inclusion complexation of syringic acid with alpha-, beta-, gamma- and heptakis(2,6-di-O-methyl)-beta-cyclodextrin. (United States)

    Song, Le Xin; Wang, Hai Ming; Xu, Peng; Yang, Yan; Zhang, Zi Qiang


    Intermolecular interactions of alpha-, beta-, gamma- and heptakis(2,6-di-O-methyl)-beta-cyclodextrin (CD) with syringic acid (Syr) in aqueous solution are investigated by fluorescence spectroscopy. The fluorescence intensity of Syr gradually increases with the addition of the CDs. The formation constants (K) of the host-guest inclusion complexes are determined using a nonlinear analysis. The association abilities of Syr with the CDs decrease in the order gamma->beta->alpha- approximately DMbeta-CD. Both the intrinsic binding abilities of the CDs and the structural effect of Syr are taken into consideration when comparing the K values. Based on the results of NMR experimental and theoretical PM3 calculations both in vacuo and in water, it is found that Syr stays near the wider rim of alpha-CD cavity. Both the number of substituted groups (NSG) in a guest and the molar volume ratio of the guest to host cavity (MVR) play an important role in forming the CD supramolecular complexes of a homologous series of phenol derivatives, such as 2-methoxylphenol (2-Mop), eugenol (Eug) and Syr, i.e., an appropriate NSG or MVR in an inclusion system, such as in 2-Mop-alpha-CD, Eug-beta-CD and Syr-gamma-CD systems, can maximize the intermolecular interaction between host and guest.

  5. Ultrafast infrared vibrational spectroscopy

    CERN Document Server

    Fayer, Michael D


    The past ten years or so have seen the introduction of multidimensional methods into infrared and optical spectroscopy. The technology of multidimensional spectroscopy is developing rapidly and its applications are spreading to biology and materials science. Edited by a recognized leader in the field and with contributions from top researchers, including experimentalists and theoreticians, this book presents the latest research methods and results and will serve as an excellent resource for other researchers.

  6. Complement activation by the amyloid proteins A beta peptide and beta 2-microglobulin

    DEFF Research Database (Denmark)

    Nybo, Mads; Nielsen, E H; Svehag, S E


    Complement activation (CA) has been reported to play a role in the pathogenesis of Alzheimer's disease (AD). To investigate whether CA may contribute to amyloidogenesis in general, the CA potential of different amyloid fibril proteins was tested. CA induced by A beta preparations containing soluble...... protein, protofilaments and some fibrils or only fibrils in a solid phase system (ELISA) was modest with a slow kinetics compared to the positive delta IgG control. Soluble A beta induced no detectable CA in a liquid phase system (complement consumption assay) while fibrillar A beta caused CA at 200 mg....../ml and higher concentrations. Soluble beta 2-microglobulin (beta 2M) purified from peritoneal dialysates was found to be as potent a complement activator as A beta in both solid and liquid phase systems while beta 2M purified from urine exhibited lower activity, a difference which may be explained...

  7. Clusters of conserved beta cell marker genes for assessment of beta cell phenotype

    DEFF Research Database (Denmark)

    Martens, Geert A; Jiang, Lei; Hellemans, Karine H;


    The aim of this study was to establish a gene expression blueprint of pancreatic beta cells conserved from rodents to humans and to evaluate its applicability to assess shifts in the beta cell differentiated state. Genome-wide mRNA expression profiles of isolated beta cells were compared to those...... of a large panel of other tissue and cell types, and transcripts with beta cell-abundant and -selective expression were identified. Iteration of this analysis in mouse, rat and human tissues generated a panel of conserved beta cell biomarkers. This panel was then used to compare isolated versus laser capture...... microdissected beta cells, monitor adaptations of the beta cell phenotype to fasting, and retrieve possible conserved transcriptional regulators....

  8. Painlev\\'e representation of Tracy-Widom$_\\beta$ distribution for $\\beta = 6$

    CERN Document Server

    Rumanov, Igor


    In \\cite{betaFP1}, we found explicit Lax pairs for the soft edge of beta ensembles with even integer values of $\\beta$. Using this general result, the case $\\beta=6$ is further considered here. This is the smallest even $\\beta$, when the corresponding Lax pair and its relation to Painlev\\'e II (PII) have not been known before, unlike cases $\\beta=2$ and $4$. It turns out that again everything can be expressed in terms of the Hastings-McLeod solution of PII. In particular, a second order nonlinear ODE for the logarithmic derivative of Tracy-Widom distribution for $\\beta=6$ involving the PII function in the coefficients, is found, which allows one to compute asymptotics for the distribution function. The ODE is a consequence of a linear system of three ODEs for which the local Painlev\\'e analysis yields series solutions with exponents in the set $4/3$, $1/3$ and $-2/3$.

  9. Highly sensitive phage-based biosensor for the detection of beta-galactosidase. (United States)

    Nanduri, Viswaprakash; Balasubramanian, Shankar; Sista, Srinivas; Vodyanoy, Vitaly J; Simonian, Aleksandr L


    Development of real-time sensor based on the target-specific probe that make possible sensitive, rapid and selective detection and monitoring of the particular antigen molecules could be of substantial importance to the many applications. Because of its high specificity to the target molecules, excellent temperature stability, and easy production, bacterial phage might serve as a powerful biorecognition probe in biosensor applications. Here, we report extremely sensitive and specific label-free direct detection of model antigen, beta-galactosidase (beta-gal), based on surface plasmon resonance (SPR) spectroscopy. The beta-gal specific landscape phage 1G40 has been immobilized on the gold surface of SPR SPREETA sensor chip through physical adsorption [V. Nanduri, A.M. Samoylova, V.Petrenko, V. Vodyanoy and A.L.Simonian, Comparison of optical and acoustic wave phage biosensors, 206th Meeting of The Electrochemical Society, Honolulu, Hawaii, October 3-8, (2004)]. Another non-specific to the beta-gal phage, a wild-type phage F8-5, was used in the reference channel. The concentration-dependent binding of beta-gal in both channels were assessed by monitoring the sensor optical response as a function of time under different experimental conditions, and the concentration of beta-gal was computed in differential mode. Concentrations of beta-gal between 10(-12) M and 10(-7) M could be readily detected, with linear part of calibration curve between 10(-9) M and 10(-6) M. When beta-gal was pre-incubated with different concentrations of free 1G40 phage prior to exposure to the biosensor, concentration-dependent inhibition was observed, indicating on biosensor high specificity toward beta-gal. Apart from a flow through mode used to deliver the samples to the surface for the SPR sensor, batch mode sensing was also employed to study the binding of beta-gal to immobilized phage on the SPR sensor surface. Experiments using a flow through mode provided more consistent results in the

  10. Electronic Spectroscopy & Dynamics

    Energy Technology Data Exchange (ETDEWEB)

    Mark Maroncelli, Nancy Ryan Gray


    The Gordon Research Conference (GRC) on Electronic Spectroscopy and Dynamics was held at Colby College, Waterville, NH from 07/19/2009 thru 07/24/2009. The Conference was well-attended with participants (attendees list attached). The attendees represented the spectrum of endeavor in this field coming from academia, industry, and government laboratories, both U.S. and foreign scientists, senior researchers, young investigators, and students. The GRC on Electronic Spectroscopy & Dynamics showcases some of the most recent experimental and theoretical developments in electronic spectroscopy that probes the structure and dynamics of isolated molecules, molecules embedded in clusters and condensed phases, and bulk materials. Electronic spectroscopy is an important tool in many fields of research, and this GRC brings together experts having diverse backgrounds in physics, chemistry, biophysics, and materials science, making the meeting an excellent opportunity for the interdisciplinary exchange of ideas and techniques. Topics covered in this GRC include high-resolution spectroscopy, biological molecules in the gas phase, electronic structure theory for excited states, multi-chromophore and single-molecule spectroscopies, and excited state dynamics in chemical and biological systems.

  11. Coincidence Auger spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Penent, F. [LCPMR, Universite Pierre et Marie Curie, 75231 Paris 5 (France) and DIAM, Universite Pierre et Marie Curie, 75252 Paris 5 (France)]. E-mail:; Lablanquie, P. [LURE, Universite Paris Sud, 91898 Orsay (France); Hall, R.I. [DIAM, Universite Pierre et Marie Curie, 75252 Paris 5 (France); Palaudoux, J. [LCPMR, Universite Pierre et Marie Curie, 75231 Paris 5 (France); Ito, K. [Photon Factory, IMSS, KEK, Tsukuba 305-0801 (Japan); Hikosaka, Y. [Photon Factory, IMSS, KEK, Tsukuba 305-0801 (Japan); IMS, Okazaki 444-8585 (Japan); Aoto, T. [Photon Factory, IMSS, KEK, Tsukuba 305-0801 (Japan); Eland, J.H.D. [Physical and Theoretical Chemistry Laboratory, South Parks Road, Oxford OX1 3DW (United Kingdom)


    Auger electron spectroscopy (AES) and photoelectron spectroscopy (PES) are (with X-ray emission spectroscopy, XES) powerful analytical tools for material science and gas phase studies. However, the interpretation of Auger spectra can be very difficult due to the number and complexity of the involved processes. A deeper analysis, that allows a better understanding of relaxation processes following inner shell ionization, is possible with coincidence Auger spectroscopy. This method gives a new insight into electron correlation and allows disentangling of complex Auger electron spectra. In this paper, we present some examples related to gas phase coincidence Auger electron spectroscopy using synchrotron radiation. The detection in coincidence of an Auger electron with a threshold photoelectron presents two main advantages which are good energy resolution and high coincidence count rates. This technique has also provided new results on double Auger decay processes. A further qualitative breakthrough has been made with the development of a new experimental set-up based on a magnetic bottle time-of-flight electron spectrometer. This opens up the field of multi-electron coincidence spectroscopy and allows a most detailed analysis with characterization of all possible decay pathways following inner shell ionization.

  12. Expression of TGF-beta1, TGF-beta2, TGF-beta3 and the receptors TGF-betaRI and TGF-betaRII in placentomes of artificially inseminated and nuclear transfer derived bovine pregnancies. (United States)

    Ravelich, S R; Shelling, A N; Wells, D N; Peterson, A J; Lee, R S F; Ramachandran, A; Keelan, J A


    Bovine nuclear transfer pregnancies are characterized by a high incidence of placental abnormalities, notably, increased placentome size and deficiencies in trophoblast cell function and establishment of placental vasculature. Alterations in gene expression during placental growth and development may contribute to the appearance of large placentomes in pregnancies derived from nuclear transfer. The placenta synthesizes a number of cytokines and growth factors, including the transforming growth factor-betas (TGF-betas) that are involved in the establishment, maintenance and/or regulation of pregnancy. All forms of TGF-beta and their receptors are present at the fetal-maternal interface of the bovine placentome, where they are thought to play an important role in regulating growth, differentiation, and function of the placenta. Using real-time RT-PCR, we have examined the expression of TGF-beta1, TGF-beta2, TGF-beta3 and the receptors TGF-betaRI and TGF-betaRII in placentomes of artificially inseminated (AI) and nuclear transfer (NT)-derived bovine pregnancies at days 50, 100 and 150 of gestation. TGF-beta1, TGF-beta2 and TGF-beta3 mRNA expression increased by 2.0-2.8-fold, while TGF-betaRI and TGF-betaRII mRNA expression decreased by 1.7-2.0-fold in NT placentomes compared to AI controls at all gestational ages examined. These findings indicate that NT placentomes may be resistant to the growth suppressive effects of TGF-betas and could contribute to the placental proliferative abnormalities observed in NT-derived placentas. Alternatively, deficiencies in placentation may provide a mechanism whereby TGF-betas are dysregulated in NT pregnancies.

  13. Broadband Dielectric Spectroscopy on Human Blood

    CERN Document Server

    Wolf, M; Lunkenheimer, P; Loidl, A


    Dielectric spectra of human blood reveal a rich variety of dynamic processes. Achieving a better characterization and understanding of these processes not only is of academic interest but also of high relevance for medical applications as, e.g., the determination of absorption rates of electromagnetic radiation by the human body. The dielectric properties of human blood are studied using broadband dielectric spectroscopy, systematically investigating the dependence on temperature and hematocrit value. By covering a frequency range from 1 Hz to 40 GHz, information on all the typical dispersion regions of biological matter is obtained. We find no evidence for a low-frequency relaxation (alpha-relaxation) caused, e.g., by counterion diffusion effects as reported for some types of biological matter. The analysis of a strong Maxwell-Wagner relaxation arising from the polarization of the cell membranes in the 1-100 MHz region (beta-relaxation) allows for the test of model predictions and the determination of variou...

  14. Biquinolino-modified beta-cyclodextrin dimers and their metal complexes as efficient fluorescent sensors for the molecular recognition of steroids. (United States)

    Liu, Yu; Song, Yun; Chen, Yong; Li, Xue-Qing; Ding, Fei; Zhong, Rui-Qin


    A series of bridged beta-cyclodextrin (beta-CyD) dimers possessing functional tethers of various lengths was synthesized in moderate yield by the treatment of 2,2'-biquinoline- 4,4'-dicarboxylic dichloride with beta-CyD or mono[6-oligo(ethylenediamino)-6-deoxy]-beta-CyDs. The products were 2,2'-biquinoline-4,4'-dicarboxy-bridged bis(6-O-beta-CyD) (8), N,N'-bis(2-aminoethyl)-2,2'-biquinoline-4,4'-dicarboxamide-bridged bis(6-amino-6-deoxy-beta-CyD) (9), and N,N'-bis(5-amino-3-azapentyl)-2,2'-biquinoline-4,4'-dicarboxamide-bridged bis(6-amino-6-deoxy-beta-CyD) (10). The reaction of 8-10 with copper perchlorate give their copper(II) complexes 11-13 in satisfactory yields of over 77 %. All the bis(beta-CyD)s 8-13 act as efficient fluorescent sensors and display remarkable fluorescence enhancement upon addition of optically inert steroids. The inclusion complexation behaviors of 8-13 when treated with the representative steroids cholate (14), deoxycholate (15), and glycocholate (16) in aqueous solution at 25 degrees C were investigated by means of UV/Vis spectroscopy, conductivity and fluorescence measurements, circular dichroism spectroscopy, and 2D NMR spectroscopy. The tether length of bis(beta-CyD) 9 allows it to adopt a cooperative host-tether-guest binding mode in which the spacer and guest are co-included in the two CyD cavities. As a result of this cooperation, 9 has a stability constant (K(s)) about 2x10(2) times higher than that of monomodified beta-CyD 4 for inclusion complexation with cholate. Metallooligo(beta-CyD)s with four beta-CyD units have enhanced binding abilities compared with monomodified beta-CyDs. These metallo compounds have binding affinities for guest steroids that are up to 50-4.1x10(3) times higher than those of CyDs 2-4. The guest-induced fluorescence enhancement of bis(CyD)s opens a new channel for the design of sensor materials. The complex stability constants of these compounds are discussed from the viewpoint of induced-fit interaction

  15. Conformational behaviour of glycomimetics: NMR and molecular modelling studies of the C-glycoside analogue of the disaccharide methyl beta-D-galactopyranosyl-(1-->3)-beta-D-glucopyranoside. (United States)

    Vidal, Paloma; Vauzeilles, Boris; Blériot, Yves; Sollogoub, Matthieu; Sinaÿ, Pierre; Jiménez-Barbero, Jesús; Espinosa, Juan F


    The conformational behaviour of the C-glycoside beta-C-Gal-(1-->3)-beta-Glc-OMe (1) has been studied using a combination of molecular mechanics and NMR spectroscopy (proton-proton coupling constants and nuclear Overhauser effects). It is shown that the C-disaccharide populates two distinctive conformational families in solution, the normal syn-psi conformation, which is the predominating conformation of parent O-glycoside 2, and the anti-psi conformation, which has not been detected for the O-disaccharide.

  16. Investigating the Influence of Mesoporosity in Zeolite Beta on its Catalytic Performance for the Conversion of Methanol to Hydrocarbons

    KAUST Repository

    Liu, Zhaohui


    Hierarchically porous zeolite Beta (Beta-MS) synthesized by a soft-templating method contains remarkable intra-crystalline mesoporosity, which reduces the diffusion length in zeolite channels down to several nanometers and alters the distribution of Al among distinct crystallographic sites. When used as a catalyst for the conversion of methanol to hydrocarbons (MTH) at 330 oC, Beta-MS exhibited a 2.7-fold larger conversion capacity, a 2.0-fold faster reaction rate, and a remarkably longer lifetime than conventional zeolite Beta (Beta-C). The superior catalytic performance of Beta-MS is attributed to its hierarchical structure, which offers full accessibility to all catalytic active sites. In contrast, Beta-C was easily deactivated because a layer of coke quickly deposited on the outer surfaces of the catalyst crystals, impeding access to interior active sites. This difference is clearly demonstrated by using electron microscopy combined with electron energy loss spectroscopy to probe the distribution of coke in the deactivated catalysts. At both low and high conversions, ranging from 20% to 100%, Beta-MS gave higher selectivity towards higher aliphatics (C4-C7) but lower ethene selectivity compared to Beta-C. Therefore, we conclude that a hierarchical structure decreases the residence time of methylbenzenes in zeolite micropores, disfavoring the propagation of the aromatic-based catalytic cycle. This conclusion is consistent with a recent report on ZSM-5 and is also strongly supported by our analysis of soluble coke species residing in the catalysts. Moreover, we identified an oxygen-containing compound, 4-methyl-benzaldehyde, in the coke, which has not been observed in the MTH reaction before.  

  17. Beta decay of the M{sub T}=-1 nucleus {sup 58}Zn studied by selective laser ionization

    Energy Technology Data Exchange (ETDEWEB)

    Jokinen, A.; Aeystoe, J. [Jyvaeskylae Univ. (Finland). Dept. of Physics; Oinonen, M. [Jyvaeskylae Univ. (Finland). Dept. of Physics]|[EP Division, CERN, CH-1211 Geneve 23 (Switzerland)] [and others; ISOLDE Collaboration


    Beta decay of {sup 58}Zn has been studied for the first time. A new laser ion-source concept has been used to produce mass-separated sources for beta and gamma spectroscopy. The half-life of {sup 58}Zn was determined to be 86(18) ms. Comparisons are made with previous data from charge-exchange reactions. Our Gamow-Teller strength to the 1{sup +} state at 1051 keV excitation in {sup 58}Cu agrees well with the value extracted from a recent ({sup 3}He,t) study. Extensive shell-model calculations are presented. (orig.) With 7 figs., 2 tabs., 33 refs.

  18. Clusters of conserved beta cell marker genes for assessment of beta cell phenotype

    DEFF Research Database (Denmark)

    Martens, Geert A; Jiang, Lei; Hellemans, Karine H;


    The aim of this study was to establish a gene expression blueprint of pancreatic beta cells conserved from rodents to humans and to evaluate its applicability to assess shifts in the beta cell differentiated state. Genome-wide mRNA expression profiles of isolated beta cells were compared to those...... microdissected beta cells, monitor adaptations of the beta cell phenotype to fasting, and retrieve possible conserved transcriptional regulators.......The aim of this study was to establish a gene expression blueprint of pancreatic beta cells conserved from rodents to humans and to evaluate its applicability to assess shifts in the beta cell differentiated state. Genome-wide mRNA expression profiles of isolated beta cells were compared to those...... of a large panel of other tissue and cell types, and transcripts with beta cell-abundant and -selective expression were identified. Iteration of this analysis in mouse, rat and human tissues generated a panel of conserved beta cell biomarkers. This panel was then used to compare isolated versus laser capture...

  19. Ellagic acid promotes A{beta}42 fibrillization and inhibits A{beta}42-induced neurotoxicity

    Energy Technology Data Exchange (ETDEWEB)

    Feng, Ying [Department of Histology and Embryology, College of Basic Medical Science, China Medical University, Shenyang 110001 (China); Tsinghua University School of Medicine, Haidian District, Beijing 100084 (China); Yang, Shi-gao; Du, Xue-ting; Zhang, Xi; Sun, Xiao-xia; Zhao, Min [Tsinghua University School of Medicine, Haidian District, Beijing 100084 (China); Sun, Gui-yuan, E-mail: [Department of Histology and Embryology, College of Basic Medical Science, China Medical University, Shenyang 110001 (China); Liu, Rui-tian, E-mail: [Tsinghua University School of Medicine, Haidian District, Beijing 100084 (China)


    Smaller, soluble oligomers of {beta}-amyloid (A{beta}) play a critical role in the pathogenesis of Alzheimer's disease (AD). Selective inhibition of A{beta} oligomer formation provides an optimum target for AD therapy. Some polyphenols have potent anti-amyloidogenic activities and protect against A{beta} neurotoxicity. Here, we tested the effects of ellagic acid (EA), a polyphenolic compound, on A{beta}42 aggregation and neurotoxicity in vitro. EA promoted A{beta} fibril formation and significant oligomer loss, contrary to previous results that polyphenols inhibited A{beta} aggregation. The results of transmission electron microscopy (TEM) and Western blot displayed more fibrils in A{beta}42 samples co-incubated with EA in earlier phases of aggregation. Consistent with the hypothesis that plaque formation may represent a protective mechanism in which the body sequesters toxic A{beta} aggregates to render them harmless, our MTT results showed that EA could significantly reduce A{beta}42-induced neurotoxicity toward SH-SY5Y cells. Taken together, our results suggest that EA, an active ingredient in many fruits and nuts, may have therapeutic potential in AD.

  20. Dielectric spectroscopy in agrophysics (United States)

    Skierucha, W.; Wilczek, A.; Szypłowska, A.


    The paper presents scientific foundation and some examples of agrophysical applications of dielectric spectroscopy techniques. The aim of agrophysics is to apply physical methods and techniques for studies of materials and processes which occur in agriculture. Dielectric spectroscopy, which describes the dielectric properties of a sample as a function of frequency, may be successfully used for examinations of properties of various materials. Possible test materials may include agrophysical objects such as soil, fruit, vegetables, intermediate and final products of the food industry, grain, oils, etc. Dielectric spectroscopy techniques enable non-destructive and non-invasive measurements of the agricultural materials, therefore providing tools for rapid evaluation of their water content and quality. There is a limited number of research in the field of dielectric spectroscopy of agricultural objects, which is caused by the relatively high cost of the respective measurement equipment. With the fast development of modern technology, especially in high frequency applications, dielectric spectroscopy has great potential of expansion in agrophysics, both in cognitive and utilitarian aspects.

  1. Dosimetry of Low-Energy Beta Radiation

    DEFF Research Database (Denmark)

    Borg, Jette

    Useful techniques and procedures for derermination of absorbed doses from exposure in a low-energy beta radiation were studied and evaluated. The four techniques included were beta spectrometry, extrapolation chamber dosimetry, Monte Carlo (MC) calculations, and exoelectron dosimetry. As a typical...... low-energy beta radiation field a moderated spectrum from a carbon-14 source was used. The measured responce of a Si(Li) detector to photons (bremsstrahlung) showed fine agreemant with the MC calculated photon response, whereas the difference between measured and MC calculated response to electrons...

  2. Scintillating bolometers for Double Beta Decay search

    CERN Document Server

    Gironi, Luca


    In the field of Double Beta Decay (DBD) searches, the use of high resolution detectors in which background can be actively discriminated is very appealing. Scintillating bolometers containing a Double Beta Decay emitter can largely fulfill this very interesting possibility. In this paper we present the latest results obtained with CdWO4 and CaMoO4 crystals. Moreover we report, for the first time, a very interesting feature of CaMoO4 bolometers: the possibility to discriminate beta-gamma events from those induced by alpha particles thanks to different thermal pulse shape.

  3. Beta-2-mikroglobulin ved medicinske sygdomme

    DEFF Research Database (Denmark)

    Hansen, P B; Olsen, Niels Vidiendal


    -interstitial nephropathy, increased quantities of beta 2M are excreted in the urine. If the rate of glomerular filtration is reduced, serum-beta 2M is increased and this is also the case in persons with increased cell division despite normal renal function. Serum-beta 2M is, therefore, raised in numerous malignant...... must be presumed to be of value not only in the diagnosis of cerebral AIDS and other (latent) CNS infections but also to demonstrate complicating cerebral malignant lymphomata at an early stage....

  4. Silent ischemia and beta-blockade

    DEFF Research Database (Denmark)

    Egstrup, K


    and should also be directed at the other coronary artery risk factors of the patients. The effects of beta-blockers, which reduce the duration and frequency of silent ischemic episodes, is well described. The effect is most pronounced in the morning, when the frequency of ischemia is highest......, and the mechanism of action seems mainly mediated through a reduction in myocardial oxygen demand. beta-Blockers have shown effectiveness in both effort-induced angina and mixed angina, and increased anti-ischemic potency may be achieved by combination therapy with a calcium antagonist. Abrupt withdrawal of beta-blockers...

  5. Dopamine beta-hydroxylase deficiency

    Directory of Open Access Journals (Sweden)

    Senard Jean-Michel


    Full Text Available Abstract Dopamine beta-hydroxylase (DβH deficiency is a very rare form of primary autonomic failure characterized by a complete absence of noradrenaline and adrenaline in plasma together with increased dopamine plasma levels. The prevalence of DβH deficiency is unknown. Only a limited number of cases with this disease have been reported. DβH deficiency is mainly characterized by cardiovascular disorders and severe orthostatic hypotension. First symptoms often start during a complicated perinatal period with hypotension, muscle hypotonia, hypothermia and hypoglycemia. Children with DβH deficiency exhibit reduced ability to exercise because of blood pressure inadaptation with exertion and syncope. Symptoms usually worsen progressively during late adolescence and early adulthood with severe orthostatic hypotension, eyelid ptosis, nasal stuffiness and sexual disorders. Limitation in standing tolerance, limited ability to exercise and traumatic morbidity related to falls and syncope may represent later evolution. The syndrome is caused by heterogeneous molecular alterations of the DBH gene and is inherited in an autosomal recessive manner. Restoration of plasma noradrenaline to the normal range can be achieved by therapy with the synthetic precursor of noradrenaline, L-threo-dihydroxyphenylserine (DOPS. Oral administration of 100 to 500 mg DOPS, twice or three times daily, increases blood pressure and reverses the orthostatic intolerance.

  6. Energetic, Structural, and Antimicrobial Analyses of [beta]-Lactam Side Chain Recognition by [beta]-Lactamases

    Energy Technology Data Exchange (ETDEWEB)

    Caselli, E.; Powers, R.A.; Blaszczak, L.C.; Wu, C.Y.E.; Prati, F.; Shoichet, B.K. (NWU)


    Penicillins and cephalosporins are among the most widely used and successful antibiotics. The emergence of resistance to these {beta}-lactams, most often through bacterial expression of {beta}-lactamases, threatens public health. To understand how {beta}-lactamases recognize their substrates, it would be helpful to know their binding energies. Unfortunately, these have been difficult to measure because {beta}-lactams form covalent adducts with {beta}-lactamases. This has complicated functional analyses and inhibitor design. To investigate the contribution to interaction energy of the key amide (R1) side chain of {beta}-lactam antibiotics, eight acylglycineboronic acids that bear the side chains of characteristic penicillins and cephalosporins, as well as four other analogs, were synthesized. These transition-state analogs form reversible adducts with serine {beta}-lactamases. Therefore, binding energies can be calculated directly from K{sub i} values. The K{sub i} values measured span four orders of magnitude against the Group I {beta}-lactamase AmpC and three orders of magnitude against the Group II {beta}-lactamase TEM-1. The acylglycineboronic acids have K{sub i} values as low as 20 nM against AmpC and as low as 390 nM against TEM-1. The inhibitors showed little activity against serine proteases, such as chymotrypsin. R1 side chains characteristic of {beta}-lactam inhibitors did not have better affinity for AmpC than did side chains characteristic of {beta}-lactam substrates. Two of the inhibitors reversed the resistance of pathogenic bacteria to {beta}-lactams in cell culture. Structures of two inhibitors in their complexes with AmpC were determined by X-ray crystallography to 1.90 {angstrom} and 1.75 {angstrom} resolution; these structures suggest interactions that are important to the affinity of the inhibitors. Acylglycineboronic acids allow us to begin to dissect interaction energies between {beta}-lactam side chains and {beta}-lactamases. Surprisingly

  7. Spectroscopy for the Masses (United States)

    Le Roy, Robert J.; Hopkins, Scott; Power, William P.; Leung, Tong; Hepburn, John


    Undergraduate students in all areas of science encounter one or more types of spectroscopy as an essential tool in their discipline, but most never take the advanced physics or chemistry courses in which the subject is normally taught. To address this problem, for over 20 years our department has been teaching a popular Introductory Spectroscopy course that assumes as background only a one-term introductory chemistry course containing a unit on atomic theory, and a familiarity with rudimentary calculus. This survey course provides an introduction to microwave, infrared, Raman, electronic, photoelectron and NMR spectroscopy in a manner that allows students to understand many of these phenomena as intuitive generalizations of the problem of a particle in a 1-D box or a particle-on-a-ring, and does not require any high level mathematics.

  8. Terahertz Spectroscopy and Imaging

    CERN Document Server

    Zeitler, Axel; Kuwata-Gonokami, Makoto


    "This book presents the current state of knowledge in the field of terahertz spectroscopy, providing a comprehensive source of information for beginners and experienced researchers alike whose interests lie in this area. The book aims to explain the fundamental physics that underpins terahertz  technology and to describe its key applications. Highlights of scientific research in the field of terahertz science are also outlined in some chapters, providing an overview as well as giving an insight into future directions for research.  Over the past decade terahertz spectroscopy has developed into one of the most rapidly growing areas of its kind, gaining an important impact across a wide range of scientific disciplines. Due to substantial advances in femtosecond laser technology, terahertz time-domain spectroscopy (THz-TDS) has established itself as the dominant spectroscopic technique for experimental scientists interested in measurements at this frequency range. In solids and liquids THz radiation is in reso...

  9. Vibrational Spectroscopy of Biomembranes (United States)

    Schultz, Zachary D.; Levin, Ira W.


    Vibrational spectroscopy, commonly associated with IR absorption and Raman scattering, has provided a powerful approach for investigating interactions between biomolecules that make up cellular membranes. Because the IR and Raman signals arise from the intrinsic properties of these molecules, vibrational spectroscopy probes the delicate interactions that regulate biomembranes with minimal perturbation. Numerous innovative measurements, including nonlinear optical processes and confined bilayer assemblies, have provided new insights into membrane behavior. In this review, we highlight the use of vibrational spectroscopy to study lipid-lipid interactions. We also examine recent work in which vibrational measurements have been used to investigate the incorporation of peptides and proteins into lipid bilayers, and we discuss the interactions of small molecules and drugs with membrane structures. Emerging techniques and measurements on intact cellular membranes provide a prospective on the future of vibrational spectroscopic studies of biomembranes.

  10. Chiral Rotational Spectroscopy

    CERN Document Server

    Cameron, Robert P; Barnett, Stephen M


    We introduce chiral rotational spectroscopy: a new technique that enables the determination of the individual optical activity polarisability components $G_{XX}'$, $G_{YY}'$, $G_{ZZ}'$, $A_{X,YZ}$, $A_{Y,ZX}$ and $A_{Z,XY}$ of chiral molecules, in a manner that reveals the enantiomeric constitution of a sample whilst yielding an incisive signal even for a racemate. Chiral rotational spectroscopy could find particular use in the analysis of molecules that are chiral by virtue of their isotopic constitution and molecules with multiple chiral centres. The principles that underpin chiral rotational spectroscopy can also be exploited in the search for molecular chirality in space, which, if found, may add weight to hypotheses that biological homochirality and indeed life itself are of cosmic origin.

  11. Systematic Risk on Istanbul Stock Exchange: Traditional Beta Coefficient Versus Downside Beta Coefficient

    Directory of Open Access Journals (Sweden)

    Gülfen TUNA


    Full Text Available The aim of this study is to test the validity of Downside Capital Asset Pricing Model (D-CAPM on the ISE. At the same time, the explanatory power of CAPM's traditional beta and D-CAPM's downside beta on the changes in the average return values are examined comparatively. In this context, the monthly data for seventy three stocks that are continuously traded on the ISE for the period 1991-2009 is used. Regression analysis is applied in this study. The research results have shown that D-CAPM is valid on the ISE. In addition, it is obtained that the power of downside beta coefficient is higher than traditional beta coefficient on explaining the return changes. Therefore, it can be said that the downside beta is superior to traditional beta in the ISE for chosen period.

  12. Synthesis of mesoporous Beta and Sn-Beta zeolites and their catalytic performances. (United States)

    Jin, Junjiang; Ye, Xinxin; Li, Yongsheng; Wang, Yanqin; Li, Liang; Gu, Jinlou; Zhao, Wenru; Shi, Jianlin


    Mesoporous Beta zeolite has been successfully prepared through hydrothermal synthesis in the presence of cationic ammonium-modified chitosan as the meso-template. Through a subsequent solid-gas reaction between highly dealuminated mesoporous Beta zeolite and SnCl4 steam at an elevated temperature, mesoporous Sn-Beta has been facilely obtained. It was revealed that the addition of cationic chitosan induced the nanocrystal aggregation to particle sizes of ∼300 nm, giving rise to the intercrystalline/interparticle mesoporosity. In the Sn-implanting procedure, Sn species were demonstrated to be doped into the framework of the resulting mesoporous Beta zeolite in a tetrahedral environment without structural collapse. Due to the micro/mesoporous structures, both mesoporous Beta and Sn-Beta exhibited superior performances in α-pinene isomerization, Baeyer-Villiger oxidation of 2-adamantanone by hydrogen peroxide and the isomerization of glucose in water, respectively.

  13. Expression of transforming growth factor beta (TGF beta) receptors and expression of TGF beta 1, TGF beta 2 and TGF beta 3 in human small cell lung cancer cell lines

    DEFF Research Database (Denmark)

    Damstrup, L; Rygaard, K; Spang-Thomsen, M;


    A panel of 21 small cell lung cancer cell (SCLC) lines were examined for the presence of Transforming growth factor beta receptors (TGF beta-r) and the expression of TGF beta mRNAs. By the radioreceptor assay we found high affinity receptors to be expressed in six cell lines. scatchard analysis...... of the binding data demonstrated that the cells bound between 4.5 and 27.5 fmol mg-1 protein with a KD ranging from 16 to 40 pM. TGF beta 1 binding to the receptors was confirmed by cross-linking TGF beta 1 to the TGF beta-r. Three classes of TGF beta-r were demonstrated, type I and type II receptors with M......(r) = 65,000 and 90,000 and the betaglycan (type III) with M(r) = 280,000. Northern blotting showed expression of TGF beta 1 mRNA in ten, TGF beta 2 mRNA in two and TGF beta 3 mRNA in seven cell lines. Our results provide, for the first time, evidence that a large proportion of a broad panel of SCLC cell...

  14. Infrared spectroscopy of stars (United States)

    Merrill, K. M.; Ridgway, S. T.


    This paper reviews applications of IR techniques in stellar classification, studies of stellar photospheres, elemental and isotopic abundances, and the nature of remnant and ejected matter in near-circumstellar regions. Qualitative IR spectral classification of cool and hot stars is discussed, along with IR spectra of peculiar composite star systems and of obscured stars, and IR characteristics of stellar populations. The use of IR spectroscopy in theoretical modeling of stellar atmospheres is examined, IR indicators of stellar atmospheric composition are described, and contributions of IR spectroscopy to the study of stellar recycling of interstellar matter are summarized. The future of IR astronomy is also considered.

  15. Beta-2 Microglobulin Kidney Disease Test (United States)

    ... Beta 2 Microglobulin, Serum or Urine Related tests: Albumin , Creatinine , BUN , Heavy Metals Were you looking for ... monitor persons with end-stage renal disease (ESRD) Excess B2M can accumulate in joint spaces ( synovitis ) in ...

  16. Probing neutrinoless double beta decay with SNO+

    CERN Document Server

    Arushanova, Evelina


    Probing neutrinoless double beta decay is one of the primary goals for SNO+, SNOLAB's multi-purpose neutrino detector. In order to achieve this goal the SNO detector has been adapted so that it can be filled with Te-loaded liquid scintillator. During the initial double beta phase the target loading is 0.3% natural Te, which equates to $\\sim790$ kg of double beta isotope. Estimating the sensitivity to neutrinoless double beta decay requires a well understood background model. For SNO+ this is provided by a comprehensive study considering all possible background contributions, whether they originate from within the liquid scintillator cocktail, the surrounding parts of the detector or other irreducible backgrounds. Given these considerations, for five years running in the initial phase, the expected sensitivity is $T_{1/2}^{0\

  17. Neutrino potential for neutrinoless double beta decay

    CERN Document Server

    Iwata, Yoritaka


    Neutrino potential for neutrinoless double beta decay is studied with focusing on its statistical property. The statistics provide a gross view of understanding amplitude of constitutional components of the nuclear matrix element.

  18. Beta cell proliferation and growth factors

    DEFF Research Database (Denmark)

    Nielsen, Jens Høiriis; Svensson, C; Møldrup, Annette


    Formation of new beta cells can take place by two pathways: replication of already differentiated beta cells or neogenesis from putative islet stem cells. Under physiological conditions both processes are most pronounced during the fetal and neonatal development of the pancreas. In adulthood little...... increase in the beta cell number seems to occur. In pregnancy, however, a marked hyperplasia of the beta cells is observed both in rodents and man. Increased mitotic activity has been seen both in vivo and in vitro in islets exposed to placental lactogen (PL), prolactin (PRL) and growth hormone (GH......). Receptors for both GH and PRL are expressed in islet cells and are upregulated during pregnancy. By mutational analysis we have identified different functional domains of the cytoplasmic part of the GH receptor. Thus the mitotic signaling only requires the membrane proximal part of the receptor...

  19. Phenomenology of neutrinoless double beta decay

    CERN Document Server

    Gómez-Cadenas, J J


    This paper reviews the current status and future outlook of neutrinoless double beta decay searches, which try to provide an answer to the fundamental question of whether neutrinos are Dirac or Majorana particles.

  20. Literature in Focus Beta Beams: Neutrino Beams

    CERN Multimedia


    By Mats Lindroos (CERN) and Mauro Mezzetto (INFN Padova, Italy) Imperial Press, 2009 The beta-beam concept for the generation of electron neutrino beams was first proposed by Piero Zucchelli in 2002. The idea created quite a stir, challenging the idea that intense neutrino beams only could be produced from the decay of pions or muons in classical neutrino beams facilities or in future neutrino factories. The concept initially struggled to make an impact but the hard work by many machine physicists, phenomenologists and theoreticians over the last five years has won the beta-beam a well-earned position as one of the frontrunners for a possible future world laboratory for high intensity neutrino oscillation physics. This is the first complete monograph on the beta-beam concept. The book describes both technical aspects and experimental aspects of the beta-beam, providing students and scientists with an insight into the possibilities o...

  1. [Beta 3 adrenergic receptor polymorphism and obesity]. (United States)

    Yoshida, T; Umekawa, T


    The beta 3-adrenoceptor plays a significant role in the control of lipolysis and thermogenesis in the brown adipose tissue of rodents and humans. In human beta 3-adrenoceptor, a Trp to Arg replacement has recently been discovered. This change which occurs at position 64, in the first coding exon, has been correlated with increased weight gain, difficulty in losing weight, insulin resistance syndrome, and worsened diabetic situation. Higher percentages of this mutation are observed in Pima Indians (over 30%) and Japanese (20%). The possible functional mechanism of Trp54Arg is reported using human HEK293 cell line stably expressing the wild type and the [Arg64] beta 3-adrenoceptor type. Beta 3-adrenoceptor agonists available for humans are been also developing. In this paper we describe these points up-to-date.

  2. [Allergy to beta-lactam antibiotics]. (United States)

    Comte, D; Petitpierre, S; Spertini, F; Bart, P-A


    Beta-lactam antibiotics allergies are common. Up to 10% of the population describe a former allergy to penicillins. However only 10 to 15% of these individuals are actually allergic. In most cases, beta-lactam antibiotics will be avoided and replaced by other antibiotics such as quinolones. This fear of a serious allergic reaction has an economic impact and may lead to the emergence of antibiotic resistance. A thorough allergic work-up can accurately determine true allergic patients. Most of the patients with a proven allergy will be able to tolerate other antibiotics belonging to the beta-lactam family. This article focuses on the management of beta-lactam allergic patients.

  3. Metabolic response to various beta-adrenoceptor agonists in beta3-adrenoceptor knockout mice: evidence for a new beta-adrenergic receptor in brown adipose tissue. (United States)

    Preitner, F; Muzzin, P; Revelli, J P; Seydoux, J; Galitzky, J; Berlan, M; Lafontan, M; Giacobino, J P


    The beta3-adrenoceptor plays an important role in the adrenergic response of brown and white adipose tissues (BAT and WAT). In this study, in vitro metabolic responses to beta-adrenoceptor stimulation were compared in adipose tissues of beta3-adrenoceptor knockout and wild type mice. The measured parameters were BAT fragment oxygen uptake (MO2) and isolated white adipocyte lipolysis. In BAT of wild type mice (-)-norepinephrine maximally stimulated MO2 4.1+/-0.8 fold. Similar maximal stimulations were obtained with beta1-, beta2- or beta3-adrenoceptor selective agonists (dobutamine 5.1+/-0.3, terbutaline 5.3+/-0.3 and CL 316,243 4.8+/-0.9 fold, respectively); in BAT of beta3-adrenoceptor knockout mice, the beta1- and beta2-responses were fully conserved. In BAT of wild type mice, the beta1/beta2-antagonist and beta3-partial agonist CGP 12177 elicited a maximal MO2 response (4.7+/-0.4 fold). In beta3-adrenoceptor knockout BAT, this response was fully conserved despite an absence of response to CL 316,243. This unexpected result suggests that an atypical beta-adrenoceptor, distinct from the beta1-, beta2- and beta3-subtypes and referred to as a putative beta4-adrenoceptor is present in BAT and that it can mediate in vitro a maximal MO2 stimulation. In isolated white adipocytes of wild type mice, (-)-epinephrine maximally stimulated lipolysis 12.1+/-2.6 fold. Similar maximal stimulations were obtained with beta1-, beta2- or beta3-adrenoceptor selective agonists (TO509 12+/-2, procaterol 11+/-3, CL 316,243 11+/-3 fold, respectively) or with CGP 12177 (7.1+/-1.5 fold). In isolated white adipocytes of beta3-adrenoceptor knockout mice, the lipolytic responses to (-)epinephrine, to the beta1-, beta2-, beta3-adrenoceptor selective agonists and to CGP 12177 were almost or totally depressed, whereas those to ACTH, forskolin and dibutyryl cyclic AMP were conserved.

  4. Theoretical spectroscopy of quasars within Karlsson's law

    CERN Document Server

    Moret-Bailly, Jacques


    The law introduced by Karlsson in spectroscopy of low-redshift quasars involves the Lyman spectrum of hydrogen atoms. Thus, it appears necessary to study the concepts introduced by a standard spectroscopy of quasars, studied here, with those deducted from $\\Lambda$-CDM.A visible absorption of a sharp and saturated spectral line in a gas requires a long path without perturbations as collisions or cosmological redshift. Spectra of absorbed, saturated lines of quasars obeying Karlsson's law mainly result from interactions of natural, thermal light radiated by quasar with relatively cold, low presure atomic hydrogen. These lines are produced by three processes: a) A conventional absorption in a relatively cold gas produces a set of lines; b) These lines are multiplied by absorption after fundamental 3K or 4K redshifts, where K is Karlsson's constant: Spectra show that redshifts 3K (or 4K) exactly bring absorbed Lyman beta (or gamma) line on Lyman alpha: redshift almost disappears, and gas lines are intensely abso...

  5. Isotope Effects in the Bonds of beta-CrOOH and beta-CrOOD

    DEFF Research Database (Denmark)

    Nørlund Christensen, A.; Hansen, P.; Lehmann, M. S.


    Samples of orthorhombic chromium oxide hydroxide, beta -CrOOH, and the deuterated compound, beta -CrOOD, were prepared hydrothermally. The crystal structures were determined by powder profile refinement technique using neutron diffraction data. Unit cells are: beta -CrOOH: a equals 4. 862(2) A, b...... equals 4. 298(a) A, c equals 2. 995(1) A; beta -CrOOD: a equals 4. 873(5) A, b equals 4. 332(7) A, c equals 2. 963(2) A, with Z equals 2. The space group is P2//1nm or Pnnm....

  6. AE activity during transient beta drops in high poloidal beta discharges (United States)

    Huang, J.; Gong, X. Z.; Ren, Q. L.; Ding, S. Y.; Qian, J. P.; Pan, C. K.; Li, G. Q.; Heidbrink, W. W.; Garofalo, A. M.; McClenaghan, J.


    Enhanced AE activity has been observed during transient beta drops in high poloidal beta DIII-D discharges with internal transport barriers (ITBs). These drops in beta are believed to be caused by n=1 external kink modes. In some discharges, beta recovers within 200 ms but, in others, beta stays suppressed. A typical discharge has βP 3, qmin 3, and q95 12. The drop in beta affects both fast ions and thermal particles, and a drop is also observed in the density and rotation. The enhanced AE activity follows the instability that causes the beta drop, is largest at the lowest beta, and subsides as beta recovers. MHD stability analysis is planned. A database study of the plasma conditions associated with the collapse will be also presented. Supported in part by the US Department of Energy under DE-FC02-04ER54698, DE-AC05-06OR23100, and by the National Natural Science Foundation of China 11575249, and the National Magnetic Confinement Fusion Program of China No. 2015GB110005.

  7. Beta adrenergic receptors in human cavernous tissue

    Energy Technology Data Exchange (ETDEWEB)

    Dhabuwala, C.B.; Ramakrishna, C.V.; Anderson, G.F.


    Beta adrenergic receptor binding was performed with /sup 125/I iodocyanopindolol on human cavernous tissue membrane fractions from normal tissue and transsexual procedures obtained postoperatively, as well as from postmortem sources. Isotherm binding studies on normal fresh tissues indicated that the receptor density was 9.1 fmoles/mg. with a KD of 23 pM. Tissue stored at room temperature for 4 to 6 hours, then at 4C in saline solution for 19 to 20 hours before freezing showed no significant changes in receptor density or affinity, and provided evidence for the stability of postmortem tissue obtained within the same time period. Beta receptor density of 2 cavernous preparations from transsexual procedures was not significantly different from normal control tissues, and showed that high concentrations of estrogen received by these patients had no effect on beta adrenergic receptor density. Displacement of /sup 125/iodocyanopindolol by 5 beta adrenergic agents demonstrated that 1-propranolol had the greatest affinity followed by ICI 118,551, zinterol, metoprolol and practolol. When the results of these displacement studies were subjected to Scatfit, non- linear regression line analysis, a single binding site was described. Based on the relative potency of the selective beta adrenergic agents it appears that these receptors were of the beta 2 subtype.

  8. Estimating Security Betas Using Prior Information Based on Firm Fundamentals

    NARCIS (Netherlands)

    Cosemans, Mathijs; Frehen, Rik; Schotman, Peter; Bauer, Rob


    We propose a hybrid approach for estimating beta that shrinks rolling window estimates toward firm-specific priors motivated by economic theory. Our method yields superior forecasts of beta that have important practical implications. First, unlike standard rolling window betas, hybrid betas carry a

  9. beta(2)-Glycoprotein I : evolution, structure and function

    NARCIS (Netherlands)

    de Groot, P. G.; Meijers, J. C. M.


    beta(2)-Glycoprotein I (beta(2)-GPI) is a protein that circulates in blood at high concentrations. The function of beta(2)-GPI has long been an enigma. More than 20 years ago, it was discovered that beta(2)-GPI is the major antigen for the circulating antibodies in the antiphospholipid syndrome. How

  10. Speculations in hadron spectroscopy

    CERN Document Server

    Richard, J M


    A selected survey is presented of the recent progress in hadron spectroscopy. This includes spin-singlet charmonium states, excitations of charmonium and open-charm mesons, double-charm baryons, and pentaquark candidates. Models proposing exotic bound states or resonances are reviewed. The sector of exotic mesons with two heavy quarks appears as particularly promising.

  11. Laser spectroscopy of protonium

    CERN Document Server

    Hayano, R S


    High-precision laser spectroscopy of protonium (pp) is one of the future experiments being considered by ASACUSA collaboration at CERN AD. A possible scheme to produce protonium in vacuum and to detect laser transitions is presented, and implications of reaching high precision are discussed. (7 refs).

  12. Broadband Transmission EPR Spectroscopy

    NARCIS (Netherlands)

    Hagen, W.R.


    EPR spectroscopy employs a resonator operating at a single microwave frequency and phase-sensitive detection using modulation of the magnetic field. The X-band spectrometer is the general standard with a frequency in the 9–10 GHz range. Most (bio)molecular EPR spectra are determined by a combination

  13. Bioacoustic Absorption Spectroscopy (United States)


    frequencies (Ching and Weston, 1971). RESULTS Measured resonance frequencies of absorption lines, which were attributed to adult (~ 1.3 khz) and juvenile ...of adult and juvenile sardines. These results suggest that bioacoustic absorption spectroscopy measurements permit isolation of juvenile from adult...from broadband tomographic transmission loss measurements over large areas . 2. Depths of sardines and contours of phytoplankton concentrations vs. time

  14. FTIR Rotational Spectroscopy. (United States)

    Woods, Ron; Henderson, Giles


    Presented are representative examples of the spectra and the analyses for a linear molecule (HC1), a symmetric top molecule (NH3), and an asymmetric top (H2O). Any combination of these projects could be incorporated in a physical chemistry or molecular spectroscopy laboratory. (RH)

  15. $\\beta$- decay of the proton-rich T$_{z} = -1/2$ nucleus, $^{71}$Kr

    CERN Document Server

    Oinonen, M; Äystö, J; Baumann, P; Didierjean, François; Honkanen, J A; Huck, A; Huyse, M; Knipper, A; Marguier, G; Novikov, Yu N; Popov, A; Ramdhane, M; Seliverstov, D M; Van Duppen, P; Walter, G


    $\\beta$- decay of the T$_{z}$ = - 1/2 nuclide $^{71}$Kr has been studied at the ISOLDE PSB Facility at CERN. $^{71}$Kr ions were produced in spallation reactions in a Nb foil using the 1 GeV proton beam and studied by means of $\\beta$-delayed proton, $\\beta$- and $\\gamma$-ray spectroscopy. The half-life and the $\\beta$-decay energy of $^{71}$Kr were determined using the decay of protons and positrons. These results: T$_{1/2}$ = 100 ± 3 ms and $Q_\\textrm{EC}$ = 10$^{14}$ ± 0.32 MeV and the first observation of the b-branch to the 207 keV level in $^{71}$Br makes the extension of the systematics of Gamow-Teller matrix elements of mirror nuclei up to A = 71 possible. Gamow-Teller strength of the same magnitude as that of the $fp$-shell mirror nuclei is observed for the ground state transition.

  16. News on $\\beta$-delayed particle emission from $^{14}$Be

    CERN Document Server

    Jeppesen, H; Borge, M J G; Cederkäll, J; Fynbo, H O U; Fedoseyev, V N; Hansper, V Y; Jonson, B; Markenroth, K; Mishin, V I; Nilsson, T; Nyman, G; Riisager, K; Tengblad, O; Wilhelmsen Rolander, K


    $\\beta$-delayed charged particles from $^{14}$Be have been measured and give an upper limit on $\\beta$-delayed $\\alpha$-particles of B($\\beta\\alpha$) < $\\,6.7\\times\\!10^{-5}$ and a tentative branching ratio on $\\beta$-delayed tritons of $7.5\\times\\!10^{-5}$ < B($\\beta$t) < $\\,3.9\\times\\!10^{-4}$. We combine the knowledge on $\\beta$-delayed particles from $^{14}$Be to deduce information on the $\\beta$-strength distribution.

  17. Continuous and Jump Betas: Implications for Portfolio Diversification

    Directory of Open Access Journals (Sweden)

    Vitali Alexeev


    Full Text Available Using high-frequency data, we decompose the time-varying beta for stocks into beta for continuous systematic risk and beta for discontinuous systematic risk. Estimated discontinuous betas for S&P500 constituents between 2003 and 2011 generally exceed the corresponding continuous betas. We demonstrate how continuous and discontinuous betas decrease with portfolio diversification. Using an equiweighted broad market index, we assess the speed of convergence of continuous and discontinuous betas in portfolios of stocks as the number of holdings increase. We show that discontinuous risk dissipates faster with fewer stocks in a portfolio compared to its continuous counterpart.

  18. Broadband Rotational Spectroscopy (United States)

    Pate, Brooks


    The past decade has seen several major technology advances in electronics operating at microwave frequencies making it possible to develop a new generation of spectrometers for molecular rotational spectroscopy. High-speed digital electronics, both arbitrary waveform generators and digitizers, continue on a Moore's Law-like development cycle that started around 1993 with device bandwidth doubling about every 36 months. These enabling technologies were the key to designing chirped-pulse Fourier transform microwave (CP-FTMW) spectrometers which offer significant sensitivity enhancements for broadband spectrum acquisition in molecular rotational spectroscopy. A special feature of the chirped-pulse spectrometer design is that it is easily implemented at low frequency (below 8 GHz) where Balle-Flygare type spectrometers with Fabry-Perot cavity designs become technologically challenging due to the mirror size requirements. The capabilities of CP-FTMW spectrometers for studies of molecular structure will be illustrated by the collaborative research effort we have been a part of to determine the structures of water clusters - a project which has identified clusters up to the pentadecamer. A second technology trend that impacts molecular rotational spectroscopy is the development of high power, solid state sources in the mm-wave/THz regions. Results from the field of mm-wave chirped-pulse Fourier transform spectroscopy will be described with an emphasis on new problems in chemical dynamics and analytical chemistry that these methods can tackle. The third (and potentially most important) technological trend is the reduction of microwave components to chip level using monolithic microwave integrated circuits (MMIC) - a technology driven by an enormous mass market in communications. Some recent advances in rotational spectrometer designs that incorporate low-cost components will be highlighted. The challenge to the high-resolution spectroscopy community - as posed by Frank De

  19. Phosphate and HEPES buffers potently affect the fibrillation and oligomerization mechanism of Alzheimer's A{beta} peptide

    Energy Technology Data Exchange (ETDEWEB)

    Garvey, Megan; Tepper, Katharina [Max-Planck-Forschungsstelle fuer Enzymologie der Proteinfaltung, Weinbergweg 22, D-06120 Halle (Saale) (Germany); Haupt, Caroline [Institute fuer Physik, Biophysik, Martin-Luther Universitaet Halle-Wittenberg, Betty-Heimann-Str. 7, D-06120 Halle (Saale) (Germany); Knuepfer, Uwe [Leibniz-Institute for Infection Biology and Natural Product Research, Beutenbergstr. 11a, D-07745 Jena (Germany); Klement, Karolin; Meinhardt, Jessica [Leibniz-Institute for Age Research (FLI), Beutenbergstr. 11, D-07745 Jena (Germany); Horn, Uwe [Leibniz-Institute for Infection Biology and Natural Product Research, Beutenbergstr. 11a, D-07745 Jena (Germany); Balbach, Jochen [Institute fuer Physik, Biophysik, Martin-Luther Universitaet Halle-Wittenberg, Betty-Heimann-Str. 7, D-06120 Halle (Saale) (Germany); Faendrich, Marcus, E-mail: [Max-Planck-Forschungsstelle fuer Enzymologie der Proteinfaltung, Weinbergweg 22, D-06120 Halle (Saale) (Germany); Bio zentrum, Martin-Luther Universitaet Halle-Wittenberg, Weinbergweg 22, D-06120 Halle (Saale) (Germany)


    Highlights: {yields} Sodium phosphate buffer accelerated A{beta}(1-40) nucleation relative to HEPES. {yields} A{beta}(1-40) fibrils formed in the two buffers show only minor structural differences. {yields} NMR revealed that A{beta}(1-40) histidine residues mediate buffer dependent changes. -- Abstract: The oligomerization of A{beta} peptide into amyloid fibrils is a hallmark of Alzheimer's disease. Due to its biological relevance, phosphate is the most commonly used buffer system for studying the formation of A{beta} and other amyloid fibrils. Investigation into the characteristics and formation of amyloid fibrils frequently relies upon material formed in vitro, predominantly in phosphate buffers. Herein, we examine the effects on the fibrillation and oligomerization mechanism of A{beta} peptide that occur due solely to the influence of phosphate buffer. We reveal that significant differences in amyloid fibrillation are observed due to fibrillation being initiated in phosphate or HEPES buffer (at physiological pH and temperature). Except for the differing buffer ions, all experimental parameters were kept constant. Fibril formation was assessed using fluorescently monitored kinetic studies, microscopy, X-ray fiber diffraction and infrared and nuclear magnetic resonance spectroscopies. Based on this set up, we herein reveal profound effects on the mechanism and speed of A{beta} fibrillation. The three histidine residues at positions 6, 13 and 14 of A{beta}(1-40) are instrumental in these mechanistic changes. We conclude that buffer plays a more significant role in fibril formation than has been generally acknowledged.

  20. Meaningful differences in spectral performance, thermal behavior, and heterogeneous catalysis between ammonium molybdate tetrahydrate and its adduct of beta-cyclodextrin. (United States)

    Song, Le Xin; Wang, Mang; Dang, Zheng; Du, Fang Yun


    A novel molecule-ion adduct of ammonium molybdate tetrahydrate (AMT) with beta-cyclodextrin (CD) was prepared in this work. Significant differences in spectral properties between AMT and the adduct AMT-beta-CD were observed by a series of experimental probes, such as powder X-ray diffraction, Fourier transformation infrared spectroscopy, and Raman spectroscopy. Field emission scanning electron microscopy showed that, although the crystal growth of AMT-beta-CD was dominated by the molecular stacking of AMT, the size and morphology of the adduct were rather different from those seen in free AMT. The difference in stacking forms was attributed to the contribution of the molecule-ion interaction between AMT and beta-CD. A drastic improvement in thermal stability of AMT and beta-CD after adduct formation was observed by thermogravimetry analysis, which was confirmed by controlled sintering measurements. This revealed that the adduct interaction between them played an important role in mediating the thermal decomposition process of the adducted components. Furthermore, our results indicated that AMT and its adduct had a different performance in the catalytic desulfurization of thiophene and its derivatives. The fact that the catalytic efficiency of AMT was decreased after adduct formation implied there was a complexation between AMT and beta-CD. Besides, several unusual molecular ions--NH(3)(+), NH(2)(+), and NH(+)--were simultaneously found with gas chromatography coupled to time-of-flight mass spectrometry of free AMT.

  1. DMPD: Immunoreceptor-like signaling by beta 2 and beta 3 integrins. [Dynamic Macrophage Pathway CSML Database

    Lifescience Database Archive (English)

    Full Text Available 17913496 Immunoreceptor-like signaling by beta 2 and beta 3 integrins. Jakus Z, Fod...or S, Abram CL, Lowell CA, Mocsai A. Trends Cell Biol. 2007 Oct;17(10):493-501. (.png) (.svg) (.html) (.csml) Show Immunore...ceptor-like signaling by beta 2 and beta 3 integrins. PubmedID 17913496 Title Immunoreceptor-

  2. Des-Lys58-beta 2m and native beta 2m in rheumatoid arthritis serum and synovial fluid

    DEFF Research Database (Denmark)

    Williams, R C; Malone, C C; Nissen, Mogens Holst;


    OBJECTIVE. Levels of beta 2-microglobulin and modified beta 2-microglobulin (Des-Lys58-beta 2m) were measured in serum and synovial fluids from patients with rheumatoid arthritis (RA) and other inflammatory joint disorders using rabbit antisera prepared against the beta 2m peptide VEHSDLSFS...

  3. Investigation of the Si doping effect in {beta}-Ga{sub 2}O{sub 3} films by co-sputtering of gallium oxide and Si

    Energy Technology Data Exchange (ETDEWEB)

    Takakura, Kenichiro, E-mail: [Kumamoto National College of Technology, 2659-2 Suya, Koushi, Kumamoto 862-1102 (Japan); Funasaki, Suguru; Tsunoda, Isao; Ohyama, Hidenori [Kumamoto National College of Technology, 2659-2 Suya, Koushi, Kumamoto 862-1102 (Japan); Takeuchi, Daisuke [National Institute of Advanced Industrial Science and Technology, 1-1-1, Umezono, Tsukuba, Ibaraki 305-8568 (Japan); Nakashima, Toshiyuki [Chuo Denshi Kogyo Co. Ltd., 4-3-1 Taishido Setagaya Tokyo 154-0004 (Japan); Shibuya, Mutsuo [Echo Mother, 312-2 Toriko, Nishihara, Aso-gun, Kumamoto 861-2401 (Japan); Murakami, Katsuya [Japan Gas Chemi, 995-1, Futa, Nishihara, Aso-gun, Kumamoto 861-2403 (Japan); Simoen, Eddy [Imec, Kapeldreef 75, B-3001 Leuven (Belgium); Claeys, Cor [Imec, Kapeldreef 75, B-3001 Leuven (Belgium); E. E. Department, KU Leuven, B-3001 Leuven (Belgium)


    A transparent electrode of Si doped {beta}-Ga{sub 2}O{sub 3} films for solar cells, flat panel displays and other devices, which consists of chemically abundant and ecological friendly elements of gallium and oxygen, was grown on silicon substrates by RF magnetron sputtering using sintered Ga{sub 2}O{sub 3} and Si target. The Si composition in the {beta}-Ga{sub 2}O{sub 3} film is determined by electron dispersive X-ray spectroscopy. The X-ray photoelectron spectroscopy peak ratio of oxygen over gallium decreases with increasing Si content. It is concluded that Si substitutes for the Ga sites in the {beta}-Ga{sub 2}O{sub 3} film.

  4. Characterization of beta-R1, a gene that is selectively induced by interferon beta (IFN-beta) compared with IFN-alpha. (United States)

    Rani, M R; Foster, G R; Leung, S; Leaman, D; Stark, G R; Ransohoff, R M


    We report preliminary characterization of a gene designated beta-R1, which is selectively expressed in response to interferon beta (IFN-beta) compared with IFN-alpha. In human astrocytoma cells, beta-R1 was induced to an equivalent extent by 10 IU/mL IFN-beta or 2500 IU/mL IFN-alpha2. To address the mechanism of this differential response, we analyzed induction of the beta-R1 gene in fibrosarcoma cells and derivative mutant cells lacking components required for signaling by type I IFNs. beta-R1 was readily induced by IFN-beta in the parental 2fTGH cell line, but not by recombinant IFN-alpha2, IFN-alpha Con1, or a mixture of IFN-alpha subtypes. IFN-alpha8 induced beta-R1 weakly. beta-R1 was not induced by IFN-beta in mutant cell lines U2A, U3A, U4A, and U6A, which lack, respectively, p48, STAT1, JAK1, and STAT2. U5A cells, which lack the Ifnar 2.2 component of the IFN-alpha and -beta receptor, also failed to express beta-R1. U1A cells are partially responsive to IFN-beta and IFN-alpha8 but lacked beta-R1 expression, indicating that TYK2 protein is essential for induction of this gene. Taken together, these results suggest that the expression of beta-R1 in response to type I IFN requires IFN-stimulated gene factor 3 plus an additional component, which is more efficiently formed on induction by IFN-beta compared with IFN-alpha.

  5. Amyloid Beta as a Modulator of Synaptic Plasticity


    Parihar, Mordhwaj S.; Gregory J. Brewer


    Alzheimer’s disease is associated with synapse loss, memory dysfunction and pathological accumulation of amyloid beta in plaques. However, an exclusively pathological role for amyloid beta is being challenged by new evidence for an essential function of amyloid beta at the synapse. Amyloid beta protein exists in different assembly states in the central nervous system and plays distinct roles ranging from synapse and memory formation to memory loss and neuronal cell death. Amyloid beta is pres...

  6. Properties of low-lying intruder states in 34Aland 34Sipopulated in the beta-decay of 34Mg

    CERN Document Server

    Lică, R; Negoită, F; Grévy, S; Mărginean, N; Desagne, Ph; Stora, T; Borcea, C; Borcea, R; Călinescu, S; Daugas, J M; Filipescu, D; Kuti, I; Fraile, L M; Franchoo, S; Gheorghe, I; Ghită, D G; Mărginean, R; Mihai, C; Mourface, P; Morel, P; Mrazek, J; Negret, A; Pietreanu, D; Sava, T; Sohler, D; Stănoiu, M; Stefan, I; Şuvăilă, R; Toma, S; Ur, C A


    The results of the IS530 experiment at ISOLDE revealed new information concerning several nuclei close to the N ≈ 20 'Island of Inversion' - 34Mg, 34Al, 34Si. The half-life of 34Mgwas found to be three times larger than the adopted value (63(1) ms instead of 20(10) ms). The beta-gamma spectroscopy of 34Mgperformed for the first time in this experiment, led to the first experimental level scheme for 34Al, also showing that the full beta strength goes through the predicted 1+ isomer in 34Al[1] and/or excited states that deexcite to it. The subsequent beta-decay of the 1+ isomer in 34Alallowed the observation of new gamma lines in 34Si, (tentatively) associated with low-spin high-energy excited states previously unobserved.

  7. Crystallization of metastable beta glycine from gas phase via the sublimation of alpha or gamma form in vacuum. (United States)

    Liu, Zhimin; Zhong, Lin; Ying, Pinliang; Feng, Zhaochi; Li, Can


    It is found that beta glycine, the metastable polymorph of glycine, can be rapidly formed from gas phase via the sublimation of its stable alpha or gamma form in vacuum. The transformation process was monitored by infrared spectroscopy and the crystal structure of the sublimate was identified by X-ray diffraction techniques. It is the first report about the transformation of stable alpha or gamma glycine into metastable beta form in "one-step" (heating then cool down spontaneously). Crystallization of beta glycine from gas phase is very different from other methods that require additives in solution. The hydrogen-bonding interaction and self-assembling of amino acid were discussed based on the observations.

  8. $\\beta$-decay study of neutron-rich Tl and Pb isotopes

    CERN Multimedia

    It is proposed to study the structure of neutron-rich nuclei beyond $^{208}$Pb. The one-proton hole $^{211-215}$Tl and the semi magic $^{213}$Pb will be produced and studied via nuclear and atomic spectroscopy searching for long-lived isomers and investigating the $\\beta$-delayed $\\gamma$- emission to build level schemes. Information on the single particle structure in $^{211-215}$Pb, especially the position of the g$_{9/2}$ and i$_{11/2}$ neutron orbitals, will be extracted along with lifetimes. The $\\beta$-decay will be complemented with the higher spin selectivity that can be obtained by resonant laser ionization to single-out the decay properties of long-living isomers in $^{211,213}$Tl and $^{213}$Pb.

  9. A new tool in peptide engineering: a photoswitchable stilbene-type beta-hairpin mimetic. (United States)

    Erdélyi, Máté; Karlén, Anders; Gogoll, Adolf


    Peptide secondary structure mimetics are important tools in medicinal chemistry, as they provide analogues of endogenous peptides with new physicochemical and pharmacological properties. The development, synthesis, photochemical investigation, and conformational analysis of a stilbene-type beta-hairpin mimetic capable of light-triggered conformational changes have been achieved. In addition to standard spectroscopic techniques (nuclear Overhauser effects, amide temperature coefficients, circular dichroism spectroscopy), the applicability of self-diffusion measurements (longitudinal eddy current delay pulsed-field gradient spin echo (LED-PGSE) NMR technique) in conformational studies of oligopeptides is demonstrated. The title compound shows photoisomerization of the stilbene chromophore, resulting in a change in solution conformation between an unfolded structure and a folded beta-hairpin.

  10. Oxidative dehydration of glycerol to acrylic acid over vanadium-impregnated zeolite beta

    Energy Technology Data Exchange (ETDEWEB)

    Pestana, Carolina F.M.; Guerra, Antonio C.O.; Turci, Cassia C. [Universidade Federal do Rio de Janeiro, RJ (Brazil). Inst. de Quimica; Ferreira, Glaucio B. [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Inst. de Quimica; Mota, Claudio J.A., E-mail: [INCT Energia e Ambiente, Universidade Federal do Rio de Janeiro, RJ (Brazil)


    The oxidative dehydration of glycerol to acrylic acid was studied over vanadium-impregnated zeolite Beta. Catalysts were prepared by wet impregnation of ammonium metavanadate over ammonium-exchanged zeolite Beta, followed by air calcination at 823 K. Impregnation reduced the specific surface area, but did not significantly affected the acidity (Bronsted and Lewis) of the zeolites. The catalytic evaluation was carried out in a fixed bed flow reactor using air as the carrier and injecting glycerol by means of a syringe pump. Acrolein was the main product, with acetaldehyde and hydroxy-acetone (acetol) being also formed. Acrylic acid was formed with approximately 25% selectivity at 548 K over the impregnated zeolites. The result can be explained by XPS (X-ray photoelectron spectroscopy) measurements, which indicated a good dispersion of the vanadium inside the pores. (author)

  11. Latest results of NEXT-DEMO, the prototype of the NEXT 100 double beta decay experiment

    CERN Document Server

    Serra, L; Martin-Albo, J; Sorel, M; Gomez-Cadenas, J J


    NEXT-DEMO is a 1:4.5 scale prototype of the NEXT100 detector, a high-pressure xenon gas TPC that will search for the neutrinoless double beta decay of $^{136}$Xe. X-ray energy depositions produced by the de-excitation of Xenon atoms after the interaction of gamma rays from radioactive sources have been used to characterize the response of the detector obtaining the spatial calibration needed for close-to-optimal energy resolution. Our result, 5.5% FWHM at 30 keV, extrapolates to 0.6% FWHM at the Q value of $^{136}$Xe. Additionally, alpha decays from radon have been used to measure several detection properties and parameters of xenon gas such as electron-ion recombination, electron drift velocity, diffusion and primary scintillation light yield. Alpha spectroscopy is also used to quantify the activity of radon inside the detector, a potential source of background for most double beta decay experiments.

  12. Remarkably stable inclusion complexes with heptakis-[6-deoxy-6-(2-aminoethylsulfanyl)]-beta-cyclodextrin. (United States)

    Gómez-Biagi, Rodolfo F; Jagt, Richard B C; Nitz, Mark


    Complexes of heptakis-[6-deoxy-6-(2-aminoethylsulfanyl)]-beta-cyclodextrin (1) and a series of common cyclodextrin guests were studied by NMR, fluorescence spectroscopy, and ITC experiments. NMR conformational analysis shows that the thioethers of 1 are positioned over the hydrophobic cavity of the cyclodextrin, increasing potential hydrophobic interactions with guest molecules. The combination of the increased hydrophobic character, the electrostatic complementarity and a hypothesized conformational change in 1 lead to a complex with the dye 2,6-ANS (5) that is over 2000 times more stable than with the native beta-cyclodextrin. One of the most stable host-guest complexes between a cyclodextrin and a small molecule measured to date was revealed between 1 and lithocholic acid (4) with an association constant of 5.5 x 10(7) M(-1).

  13. Beta-adrenoceptors in obstetrics and gynecology. (United States)

    Modzelewska, Beata


    One hundred and twenty years after the description of extracts from the adrenal medulla, the use of beta-blockers and beta-agonists evolved from antianginal drugs and tocolytics to ligand-directed signaling. Beta-blockers in the fields of obstetrics and gynecology have so far been limited to the consideration of continuing treatment of disorders of the cardiovascular system and other dysfunctions that started before pregnancy. Studies in recent years have shown that beta-adrenoceptor signaling might be crucial in carcinogenesis and metastasis, apoptosis and anoikis. On the other hand, the use of beta-adrenoceptor agonists in tocolysis is, as yet, the primary method for inhibiting premature uterine contractions. Unfortunately, the efficacy of current pharmacological treatment for the management of preterm labor is regularly questioned. Moreover, studies related to non-pregnant myometrium performed to date indicate that the rhythmic contractions of the uterus are required for menstruation and have an important role in human reproduction. In turn, abnormal uterine contractility has been linked to dysmenorrhea, a condition associated with painful uterine cramping. The benefits of the use of beta2-adrenoceptor agonists in dysmenorrhea are still unclear and should be balanced against a wide range of adverse effects recognized with this class of medication. The ideal tocolytic agent is one which is effective for the pregnant or non-pregnant woman but has no side effects on either the woman or the baby. Looking to the future with both caution and hope, the potential metamorphosis of beta3-adrenoceptor agonists from experimental tools into therapeutic drugs for tocolysis warrants attention.

  14. Beta Gyres in Global Analysis Fields

    Institute of Scientific and Technical Information of China (English)



    A three-component decomposition is applied to global analysis data to show the existence of a beta gyre,which causes Tropical Cyclone (TC) to drift from a large-scale environmental steering current.Analyses from the Global Data Assimilation and Prediction System (GDAPS) of the Korea Meteorological Administration (KMA),the Global Forecast System (GFS) of NCEP,and the Navy Operational Global Atmospheric Prediction System (NOGAPS) are used in this study.The structure of the beta gyre obtained in our analyses is in good agreement with the theoretical structure,with a cyclonic circulation to the southwest of the TC center,an anticyclonic circulation to the northeast,and a ventilation flow directed northwestward near the center.The circulation of the beta gyre is strongest at the 850-hPa level where the cyclonically swirling primary circulation is strongest,and decreases with height,in a pyramid shape similar to the primary circulation.The individual structure of the beta gyre is case- and model-dependent.At a certain analysis time,one model may clearly reveal a well-defined beta gyre,but the other models may not.Within one model,the beta gyre may be well defined at some analysis times,but not at other times.The structure of the beta gyre in the analysis field is determined by the nature of the vortex initialization scheme and the model behavior during the 6-h forecast in the operational data assimilation cycle.

  15. [Transforming growth factor-beta controls pathogenesis of Crohn disease]. (United States)

    Friess, H; di Mola, F F; Egger, B; Scheuren, A; Kleeff, J; Zimmermann, A; Büchler, M W


    The pathogenetic mechanisms which contribute to the progression of Crohn's disease are still not known. Transforming growth factor-beta (TGF-beta) and its subtypes are multifunctional polypeptides which regulate immunological processes as well as the synthesis of the extracellular matrix and fibrogenesis. In the present study, Crohn's disease tissue samples of 18 patients undergoing intestinal resection were analyzed by Northern blot analysis, in situ hybridization and immunostaining for TGF-beta 1-3 and the TGF-beta receptors type I-III (T beta R-I, T beta R-II, T beta R-III). There was a marked overexpression of TGF-beta 1, TGF-beta 3 and T beta R-II in 94% of the Crohn's disease tissue samples. TGF-beta 2 and T beta R-I ALK5 and T beta R-III were enhanced in 72%, 72% and 82% of the Crohn tissue samples, respectively. In situ hybridization and immunostaining revealed that there was frequent coexpression of TGF-beta with its signaling receptors. Our data indicate that TGF-beta and their receptors seem to be involved in the pathogenesis of Crohn's disease. Their enhanced expression might contribute to the increase in extracellular matrix resulting in fibrosis and subsequently in intestinal obstruction.

  16. Total absorption spectroscopy study of $^{92}$Rb decay: a major contributor to reactor antineutrino flux

    CERN Document Server

    Zakari-Issoufou, A -A; Porta, A; Algora, A; Tain, J L; Valencia, E; Rice, S; Bui, V M; Cormon, S; Estienne, M; Agramunt, J; Äystö, J; Bowry, M; Briz, J A; Caballero-Folch, R; Cano-Ott, D; Cucoanes, A; Elomaa, V -V; Eronen, T; Estévez, E; Farrelly, G F; Garcia, A R; Gelletly, W; Gomez-Hornillos, M B; Gorlychev, V; Hakala, J; Jokinen, A; Jordan, M D; Kankainen, A; Karvonen, P; Kolhinen, V S; Kondev, F G; Martinez, T; Mendoza, E; Molina, F; Moore, I; Perez, A; Podolyák, Zs; Penttilä, H; Regan, P H; Reponen, M; Rissanen, J; Rubio, B; Shiba, T; Sonzogni, A A; Weber, C


    The antineutrino spectra measured in recent experiments at reactors are inconsistent with calculations based on the conversion of integral beta spectra recorded at the ILL reactor. $^{92}$Rb makes the dominant contribution to the reactor spectrum in the 5-8 MeV range but its decay properties are in question. We have studied $^{92}$Rb decay with total absorption spectroscopy. Previously unobserved beta feeding was seen in the 4.5-5.5 region and the GS to GS feeding was found to be 87.5(25)%. The impact on the reactor antineutrino spectra calculated with the summation method is shown and discussed.

  17. Total Absorption Spectroscopy Study of 92Rb Decay: A Major Contributor to Reactor Antineutrino Spectrum Shape (United States)

    Zakari-Issoufou, A.-A.; Fallot, M.; Porta, A.; Algora, A.; Tain, J. L.; Valencia, E.; Rice, S.; Bui, V. M.; Cormon, S.; Estienne, M.; Agramunt, J.; ńystö, J.; Bowry, M.; Briz, J. A.; Caballero-Folch, R.; Cano-Ott, D.; Cucoanes, A.; Elomaa, V.-V.; Eronen, T.; Estévez, E.; Farrelly, G. F.; Garcia, A. R.; Gelletly, W.; Gomez-Hornillos, M. B.; Gorlychev, V.; Hakala, J.; Jokinen, A.; Jordan, M. D.; Kankainen, A.; Karvonen, P.; Kolhinen, V. S.; Kondev, F. G.; Martinez, T.; Mendoza, E.; Molina, F.; Moore, I.; Perez-Cerdán, A. B.; Podolyák, Zs.; Penttilä, H.; Regan, P. H.; Reponen, M.; Rissanen, J.; Rubio, B.; Shiba, T.; Sonzogni, A. A.; Weber, C.


    The antineutrino spectra measured in recent experiments at reactors are inconsistent with calculations based on the conversion of integral beta spectra recorded at the ILL reactor. 92Rb makes the dominant contribution to the reactor antineutrino spectrum in the 5-8 MeV range but its decay properties are in question. We have studied 92Rb decay with total absorption spectroscopy. Previously unobserved beta feeding was seen in the 4.5-5.5 region and the GS to GS feeding was found to be 87.5(25)%. The impact on the reactor antineutrino spectra calculated with the summation method is shown and discussed.

  18. Detrimental effects of beta-blockers in COPD - A concern for nonselective beta-blockers

    NARCIS (Netherlands)

    van der Woude, HJ; Zaagsma, J; Postma, DS; Winter, TH; van Hulst, M; Aalbers, R


    Introduction: beta-Blockers are known to worsen FEV1 and airway hyperresponsiveness (AHR) in patients with asthma. Both characteristics determine the outcome of COPD, a disease with frequent cardiac comorbidity requiring beta-blocker treatment. Design: A double-blind, placebo-controlled, randomized,

  19. Fourier transform infrared analysis of ceramic powders: Quantitative determination of alpha, beta, and amorphous phases of silicon nitride

    Energy Technology Data Exchange (ETDEWEB)

    Trout, T.K.; Bellama, J.M.; Brinckman, F.E.; Faltynek, R.A.


    Fourier transform infrared spectroscopy (FT-IR) forms the basis for determining the morphological composition of mixtures containing alpha, beta, and amorphous phases of silicon nitride. The analytical technique, involving multiple linear regression treatment of Kubelka-Munk absorbance values from diffuse reflectance measurements, yields specific percent composition data for the amorphous phase as well as the crystalline phases in ternary mixtures of 0--1% by weight Si/sub 3/N/sub 4/ in potassium bromide.

  20. Beta-Decay Study of ^{150}Er, ^{152}Yb, and ^{156}Yb: Candidates for a Monoenergetic Neutrino Beam Facility

    Energy Technology Data Exchange (ETDEWEB)

    Estevez Aguado, M. E. [CSIC-Universidad de Valencia; Algora, A. [CSIC-Universidad de Valencia; Rubio, B. [CSIC-Universidad de Valencia; Bernabeu, J. [CSIC-Universidad de Valencia; Nacher, E. [CSIC-Universidad de Valencia; Tain, J. L. [CSIC-Universidad de Valencia; Gadea, A. [CSIC-Universidad de Valencia; Agramunt, J. [CSIC-Universidad de Valencia; Burkard, K. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Hueller, W. [GSI-Hemholtzzentrum fur Schwerionenforschung, Darmstadt, Germany; Doring, J. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Kirchner, R. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Mukha, I. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Plettner, C. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Roeckl, E. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Grawe, H. [GSI-Hemholtzzentrum fur Schwerionenforschung, Darmstadt, Germany; Collatz, R. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Hellstrom, M. [Gesellschaft fur Schwerionenforschung (GSI), Germany; Cano-Ott, D. [CIEMAT, Madrid; Karny, M. [University of Warsaw; Janas, Z. [University of Warsaw; Gierlik, M. [University of Warsaw; Plochocki, A. [University of Warsaw; Rykaczewski, Krzysztof Piotr [ORNL; Batist, L. [Petersburg Nuclear Physics Institute, Gatchina, Russia; Moroz, F. [Petersburg Nuclear Physics Institute, Gatchina, Russia; Wittman, V. [Petersburg Nuclear Physics Institute, Gatchina, Russia; Blazhev, A. [University of Cologne; Valiente, J. J. [INFN, Laboratori Nazionali di Legnaro, Italy; Espinoza, C. [CFPT-IST, Lisbon


    The beta decays of ^{150}Er, ^{152}Yb, and ^{156}Yb nuclei are investigated using the total absorption spectroscopy technique. These nuclei can be considered possible candidates for forming the beam of a monoenergetic neutrino beam facility based on the electron capture (EC) decay of radioactive nuclei. Our measurements confirm that for the cases studied, the EC decay proceeds mainly to a single state in the daughter nucleus.

  1. Antihydrogen Experiment Gravity Interferometry Spectroscopy

    CERN Multimedia

    Gerber, S; Tietje, I C; Allkofer, Y R; Trezzi, D; Dassa, L; Rienacker, B; Khalidova, O; Ferrari, G; Krasnicky, D; Perini, D; Cerchiari, G; Belov, A; Boscolo, I; Sacerdoti, M G; Ferragut, R O; Nedelec, P; Testera, G; Hinterberger, A; Al-qaradawi, I; Malbrunot, C L S; Brusa, R S; Prelz, F; Manuzio, G; Riccardi, C; Fontana, A; Genova, P; Haider, S; Haug, F; Merkt, F; Turbabin, A; Castelli, F; Lagomarsino, V E; Doser, M; Penasa, L; Gninenko, S; Cataneo, F; Zenoni, A; Cabaret, L; Comparat, D P; Zmeskal, J; Scampoli, P; Dudarev, A; Kellerbauer, A G; Mariazzi, S; Fesel, J V; Nesteruk, K P; Carraro, C; Zavatarelli, S M

    The AEGIS experiment (Antihydrogen Experiment: Gravity, Interferometry, Spectroscopy) has the aim of carrying out the first measurement of the gravitational interaction of antimatter to a precision of 1%, by applying techniques from atomic physics, laser spectroscopy and interferometry to a beam of antihydrogen atoms. A further goal of the experiment is to carry out spectroscopy of the antihydrogen atoms in flight.

  2. Mid-infrared upconversion spectroscopy

    DEFF Research Database (Denmark)

    Tidemand-Lichtenberg, Peter; Dam, Jeppe Seidelin; Andersen, H. V.


    Mid-infrared (MIR) spectroscopy is emerging as an attractive alternative to near-infrared or visible spectroscopy. MIR spectroscopy offers a unique possibility to probe the fundamental absorption bands of a large number of gases as well as the vibrational spectra of complex molecules. In this paper...

  3. NMR spectroscopy reveals unexpected structural variation at the protein-protein interface in MHC class I molecules

    Energy Technology Data Exchange (ETDEWEB)

    Beerbaum, Monika; Ballaschk, Martin; Erdmann, Natalja [Leibniz-Institut fuer Molekulare Pharmakologie (FMP) (Germany); Schnick, Christina [Freie Universitaet Berlin, Institut fuer Immungenetik, Charite-Universitaetsmedizin Berlin (Germany); Diehl, Anne [Leibniz-Institut fuer Molekulare Pharmakologie (FMP) (Germany); Uchanska-Ziegler, Barbara; Ziegler, Andreas [Freie Universitaet Berlin, Institut fuer Immungenetik, Charite-Universitaetsmedizin Berlin (Germany); Schmieder, Peter, E-mail: [Leibniz-Institut fuer Molekulare Pharmakologie (FMP) (Germany)


    {beta}{sub 2}-Microglobulin ({beta}{sub 2}m) is a small, monomorphic protein non-covalently bound to the heavy chain (HC) in polymorphic major histocompatibility complex (MHC) class I molecules. Given the high evolutionary conservation of structural features of {beta}{sub 2}m in various MHC molecules as shown by X-ray crystallography, {beta}{sub 2}m is often considered as a mere scaffolding protein. Using nuclear magnetic resonance (NMR) spectroscopy, we investigate here whether {beta}{sub 2}m residues at the interface to the HC exhibit changes depending on HC polymorphisms and the peptides bound to the complex in solution. First we show that human {beta}{sub 2}m can effectively be produced in deuterated form using high-cell-density-fermentation and we employ the NMR resonance assignments obtained for triple-labeled {beta}{sub 2}m bound to the HLA-B*27:09 HC to examine the {beta}{sub 2}m-HC interface. We then proceed to compare the resonances of {beta}{sub 2}m in two minimally distinct subtypes, HLA-B*27:09 and HLA-B*27:05, that are differentially associated with the spondyloarthropathy Ankylosing Spondylitis. Each of these subtypes is complexed with four distinct peptides for which structural information is already available. We find that only the resonances at the {beta}{sub 2}m-HC interface show a variation of their chemical shifts between the different complexes. This indicates the existence of an unexpected plasticity that enables {beta}{sub 2}m to accommodate changes that depend on HC polymorphism as well as on the bound peptide through subtle structural variations of the protein-protein interface.

  4. Selective binding of chiral molecules of cinchona alkaloid by beta- and gamma-cyclodextrins and organoselenium-bridged bis(beta-cyclodextrin)s. (United States)

    Liu, Yu; Li, Li; Zhang, Heng-Yi; Fan, Zhi; Guan, Xu-Dong


    The inclusion complexation behavior of chiral members of cinchona alkaloid with beta- and gamma-cyclodextrins (1 and 2) and 6,6(')-trimethylenediseleno-bridged bis(beta-cyclodextrin) (3) was assessed by means of fluorescence and 2D-NMR spectroscopy. The spectrofluorometric titrations have been performed in aqueous buffer solution (pH 7.20) at 25.0 degrees C to determine the stability constants of the inclusion complexation of 1-3 with guest molecules (i.e., cinchonine, cinchonidine, quinine, and quinidine) in order to quantitatively investigate the molecular selective binding ability. The stability constants of the resulting complexes of 2 with guest molecules are larger than that of 1. As a result of cooperative binding, the stability constants of inclusion complexation of dimeric beta-cyclodextrin 3 with cinchonidine and cinchonine are higher than that of parent 1 by factor of 4.5 and 2.4, respectively. These results are discussed from the viewpoint of the size-fit and geometric complementary relationship between the host and guest.

  5. Single-electron detection and spectroscopy via relativistic cyclotron radiation

    Energy Technology Data Exchange (ETDEWEB)

    Asner, David M.; Bradley, Rich; De Viveiros Souza Filho, Luiz A.; Doe, Peter J.; Fernandes, Justin L.; Fertl, M.; Finn, Erin C.; Formaggio, Joseph; Furse, Daniel L.; Jones, Anthony M.; Kofron, Jared N.; LaRoque, Benjamin; Leber, Michelle; MCBride, Lisa; Miller, M. L.; Mohanmurthy, Prajwal T.; Monreal, Ben; Oblath, Noah S.; Robertson, R. G. H.; Rosenberg, Leslie; Rybka, Gray; Rysewyk, Devyn M.; Sternberg, Michael G.; Tedeschi, Jonathan R.; Thummler, Thomas; VanDevender, Brent A.; Woods, N. L.


    It has been understood since 1897 that accelerating charges should emit electromagnetic radiation. Cyclotron radiation, the particular form of radiation emitted by an electron orbiting in a magnetic field, was first derived in 1904. Despite the simplicity of this concept, and the enormous utility of electron spectroscopy in nuclear and particle physics, single-electron cyclotron radiation has never been observed directly. Here we demonstrate single-electron detection in a novel radiofrequency spectrometer. We observe the cyclotron radiation emitted by individual electrons that are produced with mildly-relativistic energies by a gaseous radioactive source and are magnetically trapped. The relativistic shift in the cyclotron frequency permits a precise electron energy measurement. Precise beta electron spectroscopy from gaseous radiation sources is a key technique in modern efforts to measure the neutrino mass via the tritium decay endpoint, and this work is a proof-of-concept for future neutrino mass experiments using this technique.

  6. In-Trap Spectroscopy of Charge-Bred Radioactive Ions (United States)

    Lennarz, A.; Grossheim, A.; Leach, K. G.; Alanssari, M.; Brunner, T.; Chaudhuri, A.; Chowdhury, U.; Crespo López-Urrutia, J. R.; Gallant, A. T.; Holl, M.; Kwiatkowski, A. A.; Lassen, J.; Macdonald, T. D.; Schultz, B. E.; Seeraji, S.; Simon, M. C.; Andreoiu, C.; Dilling, J.; Frekers, D.


    In this Letter, we introduce the concept of in-trap nuclear decay spectroscopy of highly charged radioactive ions and describe its successful application as a novel spectroscopic tool. This is demonstrated by a measurement of the decay properties of radioactive mass A=124 ions (here, In124 and Cs124) in the electron-beam ion trap of the TITAN facility at TRIUMF. By subjecting the trapped ions to an intense electron beam, the ions are charge bred to high charge states (i.e., equivalent to the removal of N-shell electrons), and an increase of storage times to the level of minutes without significant ion losses is achieved. The present technique opens the venue for precision spectroscopy of low branching ratios and is being developed in the context of measuring electron-capture branching ratios needed for determining the nuclear ground-state properties of the intermediate odd-odd nuclei in double-beta (ββ) decay.

  7. Precision Muonium Spectroscopy

    CERN Document Server

    Jungmann, Klaus P


    The muonium atom is the purely leptonic bound state of a positive muon and an electron. It has a lifetime of 2.2 $\\mu$s. The absence of any known internal structure provides for precision experiments to test fundamental physics theories and to determine accurate values of fundamental constants. In particular groun dstate hyperfine structure transitions can be measured by microwave spectroscopy to deliver the muon magnetic moment. The frequency of the 1s-2s transition in the hydrogen-like atom can be determined with laser spectroscopy to obtain the muon mass. With such measurements fundamental physical interactions, in particular Quantum Electrodynamics, can also be tested at highest precision. The results are important input parameters for experiments on the muon magnetic anomaly. The simplicity of the atom enables further precise experiments, such as a search for muonium-antimuonium conversion for testing charged lepton number conservation and searches for possible antigravity of muons and dark matter.

  8. Precision Muonium Spectroscopy (United States)

    Jungmann, Klaus P.


    The muonium atom is the purely leptonic bound state of a positive muon and an electron. It has a lifetime of 2.2 µs. The absence of any known internal structure provides for precision experiments to test fundamental physics theories and to determine accurate values of fundamental constants. In particular ground state hyperfine structure transitions can be measured by microwave spectroscopy to deliver the muon magnetic moment. The frequency of the 1s-2s transition in the hydrogen-like atom can be determined with laser spectroscopy to obtain the muon mass. With such measurements fundamental physical interactions, in particular quantum electrodynamics, can also be tested at highest precision. The results are important input parameters for experiments on the muon magnetic anomaly. The simplicity of the atom enables further precise experiments, such as a search for muonium-antimuonium conversion for testing charged lepton number conservation and searches for possible antigravity of muons and dark matter.

  9. Femtosecond laser spectroscopy

    CERN Document Server

    Hannaford, Peter


    As concepts and methodologies have evolved over the past two decades, the realm of ultrafast science has become vast and exciting and has impacted many areas of chemistry, biology and physics, and other fields such as materials science, electrical engineering, and optical communication. The field has recently exploded with the announcement of a series of remarkable new developments and advances. This volume surveys this recent growth in eleven chapters written by leading international researchers in the field. It includes sections on femtosecond optical frequency combs, soft x-ray femtosecond laser sources, and attosecond laser sources. In addition, the contributors address real-time spectroscopy of molecular vibrations with sub-5-fs pulses and multidimensional femtosecond coherent spectroscopies for studying molecular and electron dynamics. Novel methods for measuring and characterizing ultrashort laser pulses and ultrashort pulses of light are also described. The topics covered are revolutionizing the field...

  10. Chiral rotational spectroscopy (United States)

    Cameron, Robert P.; Götte, Jörg B.; Barnett, Stephen M.


    We introduce chiral rotational spectroscopy, a technique that enables the determination of the orientated optical activity pseudotensor components BX X, BY Y, and BZ Z of chiral molecules, in a manner that reveals the enantiomeric constitution of a sample and provides an incisive signal even for a racemate. Chiral rotational spectroscopy could find particular use in the analysis of molecules that are chiral solely by virtue of their isotopic constitution and molecules with multiple chiral centers. A basic design for a chiral rotational spectrometer together with a model of its functionality is given. Our proposed technique offers the more familiar polarizability components αX X, αY Y, and αZ Z as by-products, which could see it find use even for achiral molecules.

  11. High Resolution Laboratory Spectroscopy

    CERN Document Server

    Brünken, Sandra


    In this short review we will highlight some of the recent advancements in the field of high-resolution laboratory spectroscopy that meet the needs dictated by the advent of highly sensitive and broadband telescopes like ALMA and SOFIA. Among these is the development of broadband techniques for the study of complex organic molecules, like fast scanning conventional absorption spectroscopy based on multiplier chains, chirped pulse instrumentation, or the use of synchrotron facilities. Of similar importance is the extension of the accessible frequency range to THz frequencies, where many light hydrides have their ground state rotational transitions. Another key experimental challenge is the production of sufficiently high number densities of refractory and transient species in the laboratory, where discharges have proven to be efficient sources that can also be coupled to molecular jets. For ionic molecular species sensitive action spectroscopic schemes have recently been developed to overcome some of the limita...

  12. Spectroscopy of neutral radium

    Energy Technology Data Exchange (ETDEWEB)

    Mol, Aran; De, Subhadeep; Jungmann, Klaus; Wilschut, Hans; Willmann, Lorenz [KVI, University of Groningen, Groningen (Netherlands)


    The heavy alkaline earth atoms radium is uniquely sensitive towards parity and time reversal symmetry violations due to a large enhancement of an intrinsic permanent electric dipole moment of the nucleous or the electron. Furthermore, radium is sensitive to atomic parity violation and the nuclear anapole moment. To prepare such experiments spectroscopy of relevant atomic states need to be done. At a later stage we will build a neutral atom trap for radium. We have built an atomic beam of the short lived isotope {sup 225}Ra with a flux of several 10{sup 4} atoms/sec. We are preparing the laser spectroscopy using this beam setup. In the preparation for efficient laser cooling and trapping we have successfully trapped barium, which is similar in it's requirements for laser cooling. The techniques which we have developed with barium can be used to trap rare radium isotopes. We report on the progress of the experiments.

  13. Raman spectroscopy in astrobiology. (United States)

    Jorge Villar, Susana E; Edwards, Howell G M


    Raman spectroscopy is proposed as a valuable analytical technique for planetary exploration because it is sensitive to organic and inorganic compounds and able to unambiguously identify key spectral markers in a mixture of biological and geological components; furthermore, sample manipulation is not required and any size of sample can be studied without chemical or mechanical pretreatment. NASA and ESA are considering the adoption of miniaturised Raman spectrometers for inclusion in suites of analytical instrumentation to be placed on robotic landers on Mars in the near future to search for extinct or extant life signals. In this paper we review the advantages and limitations of Raman spectroscopy for the analysis of complex specimens with relevance to the detection of bio- and geomarkers in extremophilic organisms which are considered to be terrestrial analogues of possible extraterrestial life that could have developed on planetary surfaces.

  14. Beta-thalassemia and beta[A] globin gene haplotypes in Mexican mestizos. (United States)

    Villalobos-Arámbula, A R; Bustos, R; Casas-Castañeda, M; Gutiérrez, E; Perea, F J; Thein, S L; Ibarra, B


    B-globin haplotypes of 20 beta-thalassemia (beta-thal) and 87 beta(A) Mexican mestizo chromosomes were analyzed to ascertain the origin of the beta-thal alleles and the frequencies and distribution of the beta(A) haplotypes among northwestern Mexican mestizos. Sixteen beta-thal chromosomes carried six Mediterranean alleles [five codon 39 C-->T; two IVS1:1 G-->A; two IVS1:5 G-->A; three IVS1:110 G(A; one codon 11 (-T) and three (deltabeta)zero-thal]; the remaining four were linked to three rare alleles (two -28 A-->C and one each: -87 C-->T and initiation codon ATG-->GTG). Among the 87 beta(A) chromosomes, 17 different 5' haplotypes with frequencies for 1, 3, 2 and 5 of 39.0%, 17. 2%, 9.2% and 6.9%, respectively, were observed. The beta-haplotype analysis showed that 13 out of 16 Mediterranean chromosomes could easily be explained by gene migration; however, one codon 39 associated with haplotype 4 (----+ +-), one IVS1:1 with haplotype 1(+----++) and one IVS1:5 G-->A, may represent separate mutational events. Analysis of the rare alleles showed that the -28 A-->C mutation was associated with the commonest beta(A) haplotype in Mexican mestizos, Mediterraneans and the total world population; therefore an independent origin cannot be ruled out. The -87 C-->T and initiation codon ATG-->GTG were found with beta-haplotypes different from the reported ones, suggesting an indigenous origin.

  15. Theory and spectroscopy (United States)

    Stanton, John F.


    The interaction between quantum-mechanical theory and spectroscopy is one of the most fertile interfaces in all of science, and has a richly storied history. Of course it was spectroscopy that provided essentially all of the evidence that not all was well (or, perhaps more correctly put, complete) with the world of 19th century classical physics. From the discoveries of the dark lines in the solar spectrum by Fraunhöfer in 1814 to the curiously simple geometric formula discovered seventy years later that described the hydrogen atom spectrum, spectroscopy and spectroscopists have consistently identified the areas of atomic and molecular science that are most in need of hard thinking by theoreticians. The rest of the story, of course, is well-known: spectroscopic results were used to understand and motivate the theory of radioactivity and ultimately the quantum theory, first in its immature form that was roughly contemporaneous with the first World War, and then the Heisenberg-Schrödinger-Dirac version that has withstood the test of time. Since the basic principles of quantum mechanics ware first understood, the subject has been successfully used to understand the patterns found in spectra, and how these relate to molecular structure, symmetry, energy levels, and dynamics. But further understanding required to attain these intellectual achievements has often come only as a result of vital and productive interactions between theoreticians and spectroscopists (of course, many people have strengths in both areas). And indeed, a field that might be termed "theoretical spectroscopy" was cultivated and is now an important part of modern molecular science.

  16. Infrared spectroscopy in astronomy (United States)

    Houck, J. R.


    The use of infrared spectroscopy in astronomy has increased dramatically in the past ten years. The broad design considerations are discussed in terms of wavelength coverage and resolution. Three rough resolution ranges, lambda/Delta lambda of approximately 100, 1000 and 10,000, are identified in which various types of astronomical problems can be studied. Numerous existing systems are briefly discussed and references are given to more complete descriptions.

  17. Optical imaging and spectroscopy

    CERN Document Server

    Brady, David J


    An essential reference for optical sensor system design This is the first text to present an integrated view of the optical and mathematical analysis tools necessary to understand computational optical system design. It presents the foundations of computational optical sensor design with a focus entirely on digital imaging and spectroscopy. It systematically covers: Coded aperture and tomographic imaging Sampling and transformations in optical systems, including wavelets and generalized sampling techniques essential to digital system analysis Geometric, wave, and statis

  18. Applications in Photoacoustic Spectroscopy. (United States)


    Characterization of Titanium Dioxide Semiconductor Powders and Crystals . 84 Anatase or rutile .... ............. ... 84 Reduction of powders...PA spectroscopy of titanium dioxide (TiO21 powders and rutile crystals is discussed in Chapter IV. Anatase powders treated in U2 at temperatures above...6006C were converted to the rutile structure as confirmed by x-ray diffraction. The rutile (powder and crystals) and the anatase (.powder) TiO 2 were

  19. Dielectric spectroscopy of polyaniline

    Energy Technology Data Exchange (ETDEWEB)

    Calleja, R.D.; Matveeva, E.M. [Polytechnical Univ. of Valencia, (Spain)


    Polyaniline films (PANI) are being considered as attractive new galvanic sources, electrochromic displays, chemical sensors, etc. So far much work has been done to study their optical, electrochemical and electrical properties. However, there are still doubts about the basic electric conductivity mechanisms of PANI. The aim of this paper is to study the influence of water molecules and acid anions on the properties of PANI films by dielectric spectroscopy.

  20. 2008 Vibrational Spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Philip J. Reid


    The conference focuses on using vibrational spectroscopy to probe structure and dynamics of molecules in gases, liquids, and interfaces. The goal is to bring together a collection of researchers who share common interests and who will gain from discussing work at the forefront of several connected areas. The intent is to emphasize the insights and understanding that studies of vibrations provide about a variety of systems.

  1. Biomolecular EPR spectroscopy

    CERN Document Server

    Hagen, Wilfred Raymond


    Comprehensive, Up-to-Date Coverage of Spectroscopy Theory and its Applications to Biological SystemsAlthough a multitude of books have been published about spectroscopy, most of them only occasionally refer to biological systems and the specific problems of biomolecular EPR (bioEPR). Biomolecular EPR Spectroscopy provides a practical introduction to bioEPR and demonstrates how this remarkable tool allows researchers to delve into the structural, functional, and analytical analysis of paramagnetic molecules found in the biochemistry of all species on the planet. A Must-Have Reference in an Intrinsically Multidisciplinary FieldThis authoritative reference seamlessly covers all important bioEPR applications, including low-spin and high-spin metalloproteins, spin traps and spin lables, interaction between active sites, and redox systems. It is loaded with practical tricks as well as do's and don'ts that are based on the author's 30 years of experience in the field. The book also comes with an unprecedented set of...

  2. Layman friendly spectroscopy (United States)

    Sentic, Stipo; Sessions, Sharon

    Affordable consumer grade spectroscopes (e.g. SCiO, Qualcomm Tricorder XPRIZE) are becoming more available to the general public. We introduce the concepts of spectroscopy to the public and K12 students and motivate them to delve deeper into spectroscopy in a dramatic participatory presentation and play. We use diffraction gratings, lasers, and light sources of different spectral properties to provide a direct experience of spectroscopy techniques. Finally, we invite the audience to build their own spectroscope--utilizing the APS SpectraSnapp cell phone application--and study light sources surrounding them in everyday life. We recontextualize the stigma that science is hard (e.g. ``Math, Science Popular Until Students Realize They're Hard,'' The Wall Street Journal) by presenting the material in such a way that it demonstrates the scientific method, and aiming to make failure an impersonal scientific tool--rather than a measure of one's ability, which is often a reason for shying away from science. We will present lessons we have learned in doing our outreach to audiences of different ages. This work is funded by the APS Outreach Grant ``Captain, we have matter matters!'' We thank New Mexico Tech Physics Department and Physics Club for help and technical equipment.

  3. Substitution of isoleucine-31 by helical-breaking proline abolishes oxidative stress and neurotoxic properties of Alzheimer's amyloid beta-peptide. (United States)

    Kanski, Jaroslaw; Aksenova, Marina; Schöneich, Christian; Butterfield, D Allan


    Alzheimer's disease (AD) brain is characterized by excess deposition of the 42-amino acid amyloid beta-peptide [A(beta)(1-42)]. AD brain is under intense oxidative stress, and we have previously suggested that A(beta)(1-42) was associated with this increased oxidative stress. In addition, we previously demonstrated that the single methionine residue of A(beta)(1-42), residue 35, was critical for the oxidative stress and neurotoxic properties of this peptide. Others have shown that the C-terminal region of A(beta)(1-42) is helical in aqueous micellar solutions, including that part of the protein containing Met35. Importantly, Cu(II)-binding induces alpha-helicity in A(beta) in aqueous solution. Invoking the i + 4 rule of helices, we hypothesized that the carbonyl oxygen of Ile31 would interact with the S atom of Met35 to change the electronic environment of the sulfur such that molecular oxygen could lead to the production of a sulfuramyl free radical on Met35. If this hypothesis is correct, a prediction would be that breaking the helical interaction of Ile31 and Met35 would abrogate the oxidative stress and neurotoxic properties of A(beta)(1-42). Accordingly, we investigated A(beta)(1-42) in which the Ile31 residue was replaced with the helix-breaking amino acid, proline. The alpha-helical environment around Met35 was completely abolished as indicated by circular dichroism (CD)-spectroscopy. As a consequence, the aggregation, oxidative stress, Cu(II) reduction, and neurotoxic properties of A(beta)(1-42)I31P were completely altered compared to native A(beta)(1-42). The results presented here are consistent with the notion that interaction of Ile31 with Met35 may play an important role in the oxidative processes of Met35 contributing to the toxicity of the peptide.

  4. Earle K. Plyler Prize for Molecular Spectroscopy and Dynamics Lecture: 2D IR Spectroscopy of Peptide Conformation (United States)

    Tokmakoff, Andrei


    Descriptions of protein and peptide conformation are colored by the methods we use to study them. Protein x-ray and NMR structures often lead to impressions of rigid or well-defined conformations, even though these are dynamic molecules. The conformational fluctuations and disorder of proteins and peptides is more difficult to quantify. This presentation will describe an approach toward characterizing and quantifying structural heterogeneity and disorder in peptides using 2D IR spectroscopy. Using amide I vibrational spectroscopy, isotope labeling strategies, and computational modeling based on molecular dynamics simulations and Markov state models allows us to characterize distinct peptide conformers and conformational variation. The examples illustrated include the beta-hairpin tripzip2 and elastin-like peptides.

  5. Raman study of the formation of beta silicomolybdic acid supported on silica, prepared by impregnation method (United States)

    Phuc, Nguyen Huu Huy; Ohkita, Hironobu; Mizushima, Takanori; Kakuta, Noriyoshi


    Beta silicomolibdic acid/silica (β-SMA, a metastable form of silicomolybdic acid - H4SiMo12O40) forms by the impregnation of fumed silica into molybdenum solution obtained by hydrolyzation of MoO2Cl2. β-SMA/silica is found to be stable up to 300 °C after calcination for 1 h due to the existence of an interlayer MoO3 between silica surface and β-SMA. Structures of molybdenum species in the preparation process (including precursor solution) were analyzed by Raman spectroscopy and XRD.

  6. Study of the deuteron emission in the $\\beta$-decay of $^{6}$He

    CERN Multimedia

    Karny, M; Tengblad, O; Riisager, K; Perkowski, J; Garcia borge, M J; Raabe, R; Kowalska, M; Fynbo, H O U; Perea martinez, A; Ter-akopian, G; Huyse, M L

    The main goal of the present proposal is to measure the continuous spectrum of deuterons emitted in the $\\beta$-decay of $^{6}$He. In particular, we want to focus on the low energy part of the spectrum, below 400 keV, which could not be accessed by all previous experiments. For the decay spectroscopy the Warsaw Optical Time Projection Chamber (OTPC) will be used. The bunches of $^{6}$He ions produced by REX-ISOLDE facility will be implanted into the active volume of the OTPC, where the rare events of deuteron emission will be recorded, practically background free.

  7. Degradation of Beta Cloth Covering for a Battery Orbital Replacement Unit in Low Earth Orbit (United States)

    Gaier, James R.; Waters, Deborah L.; Baldwin, Sammantha; Folz, Angela D.; Loos, Alyssa


    Samples from the beta cloth cover for a battery orbit replaceable unit from the International Space Station (ISS) were characterized using optical and electron microscopy, UV-vis-NIR spectrophotometry, and x-ray energy dispersive spectroscopy. Results showed that in areas where the fabric was exposed to solar radiation the absorptance increased by as much as 20 percent, and the peak difference was in the ultraviolet, indicating that the increased absorptance may have been due to radiation. The emissivity of the material over a temperature range of 300 to 700 K was essentially unchanged.

  8. Estimating beta-mixing coefficients via histograms

    CERN Document Server

    McDonald, Daniel J; Schervish, Mark


    The literature on statistical learning for time series often assumes asymptotic independence or "mixing" of data sources. Beta-mixing has long been important in establishing the central limit theorem and invariance principle for stochastic processes; recent work has identified it as crucial to extending results from empirical processes and statistical learning theory to dependent data, with quantitative risk bounds involving the actual beta coefficients. There is, however, presently no way to actually estimate those coefficients from data; while general functional forms are known for some common classes of processes (Markov processes, ARMA models, etc.), specific coefficients are generally beyond calculation. We present an l1-risk consistent estimator for the beta-mixing coefficients, based on a single stationary sample path. Since mixing coefficients involve infinite-order dependence, we use an order-d Markov approximation. We prove high-probability concentration results for the Markov approximation and show...

  9. Neutrinoless double beta decay from lattice QCD

    CERN Document Server

    Nicholson, Amy; Chang, Chia Cheng; Clark, M A; Joo, Balint; Kurth, Thorsten; Rinaldi, Enrico; Tiburzi, Brian; Vranas, Pavlos; Walker-Loud, Andre


    While the discovery of non-zero neutrino masses is one of the most important accomplishments by physicists in the past century, it is still unknown how and in what form these masses arise. Lepton number-violating neutrinoless double beta decay is a natural consequence of Majorana neutrinos and many BSM theories, and many experimental efforts are involved in the search for these processes. Understanding how neutrinoless double beta decay would manifest in nuclear environments is key for understanding any observed signals. In these proceedings we present an overview of a set of one- and two-body matrix elements relevant for experimental searches for neutrinoless double beta decay, describe the role of lattice QCD calculations, and present preliminary lattice QCD results.

  10. BETASCAN: probable beta-amyloids identified by pairwise probabilistic analysis.

    Directory of Open Access Journals (Sweden)

    Allen W Bryan


    Full Text Available Amyloids and prion proteins are clinically and biologically important beta-structures, whose supersecondary structures are difficult to determine by standard experimental or computational means. In addition, significant conformational heterogeneity is known or suspected to exist in many amyloid fibrils. Recent work has indicated the utility of pairwise probabilistic statistics in beta-structure prediction. We develop here a new strategy for beta-structure prediction, emphasizing the determination of beta-strands and pairs of beta-strands as fundamental units of beta-structure. Our program, BETASCAN, calculates likelihood scores for potential beta-strands and strand-pairs based on correlations observed in parallel beta-sheets. The program then determines the strands and pairs with the greatest local likelihood for all of the sequence's potential beta-structures. BETASCAN suggests multiple alternate folding patterns and assigns relative a priori probabilities based solely on amino acid sequence, probability tables, and pre-chosen parameters. The algorithm compares favorably with the results of previous algorithms (BETAPRO, PASTA, SALSA, TANGO, and Zyggregator in beta-structure prediction and amyloid propensity prediction. Accurate prediction is demonstrated for experimentally determined amyloid beta-structures, for a set of known beta-aggregates, and for the parallel beta-strands of beta-helices, amyloid-like globular proteins. BETASCAN is able both to detect beta-strands with higher sensitivity and to detect the edges of beta-strands in a richly beta-like sequence. For two proteins (Abeta and Het-s, there exist multiple sets of experimental data implying contradictory structures; BETASCAN is able to detect each competing structure as a potential structure variant. The ability to correlate multiple alternate beta-structures to experiment opens the possibility of computational investigation of prion strains and structural heterogeneity of amyloid

  11. Synthesis of {beta}-haloacids by radiation

    Energy Technology Data Exchange (ETDEWEB)

    Albarran, G.; Negron-Mendoza, A. [Instituto de Ciencias y Artes, Chiapas (Mexico). Escuela de Biologia


    Hydrogen halides add to alkenes yielding the corresponding haloalkane. The addition of HX ordinally follows the course of Markownikoff`s rule. In this paper we analyze the HBr addition reaction to unsaturated carboxylic acids. The course of the addition is anti-Markownikoff. This implies that the reaction goes through free radical mechanism and the haloacid formed is in the {beta}-position. The acids under study are fumaric, itaconic, citraconic acids and the methyl ester of fumaric acid. The {beta}-haloacids formed are easily purified from the bulk solutions. (author).

  12. Volume Effects in Discrete beta functions

    CERN Document Server

    Liu, Yuzhi; Zou, Haiyuan


    We calculate discrete beta functions corresponding to the two-lattice matching for the 2D O(N) models and Dyson's hierarchical model. We describe and explain finite-size effects such as the appearance of a nontrivial infrared fixed point that goes to infinity at infinite volume or the merging of an infrared and an ultraviolet fixed point. We present extensions of the RG flows to the complex coupling plane. We discuss the possibility of constructing a continuous beta function from the discrete one by using functional conjugation methods. We briefly discuss the relevance of these findings for the search of nontrivial fixed points in multiflavor lattice gauge theory models.

  13. Population screening for beta-thalassaemia. (United States)

    Flatz, S D; Flatz, G


    The graphic recording of time to 50% haemolysis in a glycerine-saline solution is a simple, reproducible method of determining erythrocyte osmotic fragility. Studies on a normal population yielded an upper limit of normal of 90 s. In 250 healthy males from Northern Thailand all 19 with beta-thalassaemia minor had abnormal osmotic indices, and the value of the test was confirmed in beta-thalassaemia heterozygotes in Europe. Of 23 patients with iron deficiency 18 had abnormal osmotic indices. However, this is not thought likely to be a significant source of false positives in the screening of populations at risk of haemoglobinopathies but in whom iron deficiency is rare.

  14. Detecting Double Beta Decays Using Nuclear Emulsions

    CERN Document Server

    Dracos, Marcos


    Neutrino nature and absolute mass scale are major questions in particle physics which cannot be addressed by the present neutrino oscillation program. To answer these two questions, several neutrinoless double beta decay experiments are underway or planed for the near future. These experiments, mainly use bolometric techniques or gaseous counters coupled with scintillator detectors. The energy resolution is better in bolometric experiments but experiments coupling tracking with calorimetry have the advantage of observing the two electron tracks and remove many background sources. Here, we present a proposal of using nuclear emulsions to observe double beta decays. This technique has the advantage of precise tracking and vertexing even for low energy electrons.

  15. American ginseng modulates pancreatic beta cell activities

    Directory of Open Access Journals (Sweden)

    Luo Luguang


    Full Text Available Abstract The mechanism of the beneficial effects of Panax quinquefolius (Xiyangshen, American ginseng on diabetes is yet to be elucidated. Recent studies show that Panax quinquefolius increases insulin production and reduces the death of pancreatic beta cells. Mechanism studies indicate that Panax quinquefolius improves cell's immuno-reactivity and mitochondrial function through various factors. Clinical studies show that Panax quinquefolius improves postprandial glycemia in type 2 diabetic patients. Further studies to identify the component(s of Panax quinquefolius linked with pancreatic islets/beta cells in vitro and in vivo are warranted for better understanding of the full effects of Panax quinquefolius.

  16. The NEXT double beta decay experiment (United States)

    Laing, A.; NEXT Collaboration


    NEXT (Neutrino Experiment with a Xenon TPC) is a neutrinoless double-beta (ββ0v) decay experiment at Laboratorio Subterraneo de Canfranc (LSC). It is an electroluminescent Time Projection Chamber filled with high pressure 136Xe gas with separated function capabilities for calorimetry and tracking. Energy resolution and background suppression are the two key features of any neutrinoless double beta decay experiment. NEXT has both good energy resolution (review NEXT R&D results, the status of detector commissioning at LSC and the NEXT physics case.

  17. Helicity and nuclear $\\beta$ decay correlations

    CERN Document Server

    Hong, Ran; García, Alejandro


    We present simple derivations of nuclear $\\beta$-decay correlations with an emphasis on the special role of helicity. This provides a good opportunity to teach students about helicity and chirality in particle physics through exercises using simple aspects of quantum mechanics. In addition, this paper serves as an introduction to nuclear $\\beta$-decay correlations from both a theoretical and experimental vantage. This article can be used to introduce students to ongoing experiments searching for hints of new physics in the low-energy precision frontier.

  18. Beta-decay of {sup 56}Cu

    Energy Technology Data Exchange (ETDEWEB)

    Ramdhane, M.; Baumann, P.; Knipper, A.; Walter, G. [Institute de Recherches Subatomiques, 67 - Strasbourg (France); Janas, Z.; Plochocki, A. [Warsaw Univ. (Poland). Inst. of Experimental Physics; Aeystoe, J.; Dendooven, P.; Jokinen, A.; Oinonen, M.; Pentilae, H. [Jyvaeskylae Univ. (Finland); Liu, W.; Grawe, H.; Hu, Z.; Kirchner, R.; Klepper, O.; Roeckl, E. [Gesellschaft fuer Schwerionenforschung mbH, Darmstadt (Germany); Gorska, M. [Warsaw Univ. (Poland). Inst. of Experimental Physics]|[Gesellschaft fuer Schwerionenforschung mbH, Darmstadt (Germany); Fujita, Y. [Osaka Univ. (Japan); Brown, B.A. [Michigan State Univ., East Lansing, MI (United States)


    By measuring positrons and {beta}-delayed {gamma}-rays emitted from mass-separated sources, the decay of {sup 56}Cu(4{sup +},T{sub z}=-1,T=1) to states in the doubly-magic nucleus {sup 56}Ni was studied for the first time. The half-life of {sup 56}Cu was measured to be 78(15) ms, and four {beta}-delayed {gamma}-rays were assigned to its decay. The resulting experimental data on Fermi and Gamow-Teller strength are compared with shell-model predictions. (orig.)

  19. Simulated progress in double-beta decay

    Energy Technology Data Exchange (ETDEWEB)

    Miley, H.S. (Pacific Northwest Laboratory, Battelle Blvd., Richland, WA 99352 (United States)); Arthur, R.J. (Pacific Northwest Laboratory, Battelle Blvd., Richland, WA 99352 (United States)); Avignone, F.T. (University of South Carolina, Columbia, SC 29208 (United States)); Barabash, A.S. (Institute for Theoretical and Experimental Physics, 117 259 Moscow (Russian Federation)); Brodzinski, R.L. (Pacific Northwest Laboratory, Battelle Blvd., Richland, WA 99352 (United States)); Collar, J.I. (University of South Carolina, Columbia, SC 29208 (United States)); Courant, H. (University of Minnesota, Minneapolis, MN 55455 (United States)); Garcia, E. (University of Zaragoza, Zaragoza (Spain)); Guerard, C.K. (University of South Carolina, Columbia, SC 29208 (United States)); Hensley, W.K. (Pacific Northwest Laboratory, Battelle Blvd., Richland, WA 99352 (United States)); Kirpichnikov, I.V. (Institute for Theoretical and Experimental Physics, 117 259 Moscow (Russian Federation)); Kl


    A Monte Carlo code has been developed to accurately simulate double-beta decay measurements. Coincident gamma rays, beta spectra, and angular correlations have been added to adequately simulate a complete [sup 100]Mo nuclear decay and provide corrections to experimentally determined detector efficiencies. This code has been used to strip certain low-background spectra obtained in the Homestake gold mine in Lead, SD, for the purpose of extremely sensitive materials assay for the construction of new, large, enriched germanium detectors. Assays as low as 9[mu]Bq/g of [sup 210]Pb in lead shielding were obtained. ((orig.))

  20. Simulated progress in double-beta decay (United States)

    Miley, H. S.; Arthur, R. J.; Avignone, F. T.; Barabash, A. S.; Brodzinski, R. L.; Collar, J. J.; Courant, H.; Garcia, E.; Guerard, C. K.; Hensley, W. K.; Kirpichnikov, I. V.; Klimenko, A. A.; Morales, A.; Morales, J.; Nunez-Lagos, R.; Osetrov, S. B.; Pogosov, V. S.; Puimedon, J.; Reeves, J. H.; Ruddick, K.; Saenz, C.; Salinas, A.; Sarsa, M. L.; Smolnikov, A. A.; Starostin, A. S.; Tamanyan, A. G.; Umatov, V. I.; Vasiliev, S. I.; Villar, J. A.


    A Monte Carlo code has been developed to accurately simulate double-beta decay measurements. Coincident gamma rays, beta spectra, and angular correlations have been added to adequately simulate a complete 100Mo nuclear decay and provide corrections to experimentally determined detector efficiencies. This code has been used to strip certain low-background spectra obtained in the Homestake gold mine in Lead, SD, for the purpose of extremely sensitive materials assay for the construction of new, large, enriched germanium detectors. Assays as low as 9 μBq/g of 210Pb in lead shielding were obtained.

  1. Atypical beta(s) haplotypes are generated by diverse genetic mechanisms. (United States)

    Zago, M A; Silva, W A; Dalle, B; Gualandro, S; Hutz, M H; Lapoumeroulie, C; Tavella, M H; Araujo, A G; Krieger, J E; Elion, J; Krishnamoorthy, R


    The majority of the chromosomes with the beta(S) gene have one of the five common haplotypes, designated as Benin, Bantu, Senegal, Cameroon, and Arab-Indian haplotypes. However, in every large series of sickle cell patients, 5-10% of the chromosomes have less common haplotypes, usually referred to as "atypical" haplotypes. In order to explore the genetic mechanisms that could generate these atypical haplotypes, we extended our analysis to other rarely studied polymorphic markers of the beta(S)-gene cluster, in a total of 40 chromosomes with uncommon haplotypes from Brazil and Cameroon. The following polymorphisms were examined: seven restriction site polymorphisms of the epsilongammadeltabeta-cluster, the pre-(G)gamma framework sequence including the 6-bp deletion/insertion pattern, HS-2 LCR (AT)xR(AT)y and pre-beta (AT)xTy repeat motifs, the GC/TT polymorphism at -1105-1106 of (G)gamma-globin gene, the C/T polymorphism at -551 of the beta-globin gene, and the intragenic beta-globin gene framework. Among the Brazilian subjects, the most common atypical structure (7/16) was a Bantu 3'-subhaplotype associated with different 5'-sequences, while in two chromosomes a Benin 3'-subhaplotype was associated with two different 5'-subhaplotypes. A hybrid Benin/Bantu configuration was also observed. In three chromosomes, the atypical haplotype differed from the typical one by the change of a single restriction site. In 2/134 chromosomes identified as having a typical Bantu RFLP-haplotype, a discrepant LCR repeat sequence was observed, probably owing to a crossover 5' to the epsilon-gene. Among 80 beta(S) chromosomes from Cameroon, 22 were associated with an atypical haplotype. The most common structure was represented by a Benin haplotype (from the LCR to the beta-gene) with a non-Benin segment 3' to the beta-globin gene. In two cases a Bantu LCR was associated with a Benin haplotype and a non-Benin segment 3' to the beta-globin gene. In three other cases, a more complex

  2. Therapeutic haemoglobin synthesis in beta-thalassaemic mice expressing lentivirus-encoded human beta-globin. (United States)

    May, C; Rivella, S; Callegari, J; Heller, G; Gaensler, K M; Luzzatto, L; Sadelain, M


    The stable introduction of a functional beta-globin gene in haematopoietic stem cells could be a powerful approach to treat beta-thalassaemia and sickle-cell disease. Genetic approaches aiming to increase normal beta-globin expression in the progeny of autologous haematopoietic stem cells might circumvent the limitations and risks of allogeneic cell transplants. However, low-level expression, position effects and transcriptional silencing hampered the effectiveness of viral transduction of the human beta-globin gene when it was linked to minimal regulatory sequences. Here we show that the use of recombinant lentiviruses enables efficient transfer and faithful integration of the human beta-globin gene together with large segments of its locus control region. In long-term recipients of unselected transduced bone marrow cells, tetramers of two murine alpha-globin and two human betaA-globin molecules account for up to 13% of total haemoglobin in mature red cells of normal mice. In beta-thalassaemic heterozygous mice higher percentages are obtained (17% to 24%), which are sufficient to ameliorate anaemia and red cell morphology. Such levels should be of therapeutic benefit in patients with severe defects in haemoglobin production.

  3. Nuclear Matrix Elements for the $\\beta\\beta$ Decay of the $^{76}$Ge

    CERN Document Server

    Brown, B A; Horoi, M


    The nuclear matrix elements for two-neutrino double-beta (2 n$\\beta\\beta$ ) and zero-neutrino double-beta (0 n$\\beta\\beta$) decay of 76 Ge are evaluated in terms of the configuration interaction (CI), quasiparticle random phase approximation (QRPA) and interacting boson model (IBM) methods. We show that the decomposition of the matrix elements in terms of interemediate states in 74 Ge is dominated by ground state of this nucleus. We consider corrections to the CI results that arise from configurations admixtures involving orbitals out-side of the CI configuration space by using results from QRPA, many-body-perturbation theory, and the connections to related observables. The CI two-neutrino matrix element is reduced due to the inclusion of spin-orbit partners, and to many-body correlations connected with Gamow-Teller beta decay. The CI zero-neutrino matrix element for the heavy neutrino is enhanced due to particle-particle correlations that are connected with the odd-even oscillations in the nuclear masse...

  4. Transforming growth factor-beta (TGF-beta) and programmed cell death in the vertebrate retina. (United States)

    Duenker, Nicole


    Programmed cell death (PCD) is a precisely regulated phenomenon essential for the homeostasis of multicellular organisms. Developmental systems, particularly the nervous system, have provided key observations supporting the physiological role of PCD. We have recently shown that transforming growth factor-beta (TGF-beta) plays an important role in mediating ontogenetic PCD in the nervous system. As part of the central nervous system the developing retina serves as an ideal model system for investigating apoptotic processes during neurogenesis in vivo as it is easily accessible experimentally and less complex due to its limited number of different neurons. This review summarizes data indicating a pivotal role of TGF-beta in mediating PCD in the vertebrate retina. The following topics are discussed: expression of TGF-beta isoforms and receptors in the vertebrate retina, the TGF-beta signaling pathway, functions and molecular mechanisms of PCD in the nervous system, TGF-beta-mediated retinal apoptosis in vitro and in vivo, and interactions of TGF-beta with other pro- and anti-apoptotic factors.

  5. Beneficial effects of beta-blockers on left ventricular function and cellular energy reserve in patients with heart failure. (United States)

    Spoladore, Roberto; Fragasso, Gabriele; Perseghin, Gianluca; De Cobelli, Francesco; Esposito, Antonio; Maranta, Francesco; Calori, Giliola; Locatelli, Massimo; Lattuada, Guido; Scifo, Paola; Del Maschio, Alessandro; Margonato, Alberto


    Beta-blockers have been shown to improve left ventricular (LV) function in patients with heart failure. The aim of this study is to non-invasively assess, by means of in vivo 31P-magnetic resonance spectroscopy (31P-MRS), the effects of beta-blockers on LV cardiac phosphocreatine and adenosine triphosphate (PCr/ATP) ratio in patients with heart failure. Ten heart failure patients on full medical therapy were beta-blocked by either carvedilol or bisoprolol. Before and after 3 months of treatment, exercise testing, 2D echocardiography, MRS, New York Heart Association (NYHA) class, ejection fraction (EF), maximal rate-pressure product and exercise metabolic equivalent system (METS) were evaluated. Relative concentrations of PCr and ATP were determined by cardiac 31P-MRS. After beta-blockade, NYHA class decreased (from 2.2 ± 0.54 to 1.9 ± 0.52, P = 0.05), whereas EF (from 33 ± 7 to 44 ± 6%, P = 0.0009) and METS (from 6.74 ± 2.12 to 8.03 ± 2.39, P = 0.01) increased. Accordingly, the mean cardiac PCr/ATP ratio increased by 33% (from 1.48 ± 0.22 to 1.81 ± 0.48, P = 0.03). Beta-blockade-induced symptomatic and functional improvement in patients with heart failure is associated to increased PCr/ATP ratio, indicating preservation of myocardial high-energy phosphate levels.

  6. {beta} decay of the proton-rich T{sub z}={minus}1/2 nucleus, {sup 71}Kr

    Energy Technology Data Exchange (ETDEWEB)

    Oinonen, M.; Aeystoe, J.; Honkanen, A. [Department of Physics, University of Jyvaeskylae, P.O. Box 35, FIN-40351, Jyvaeskylae (Finland); Jokinen, A.; Aeystoe, J. [CERN, PPE Division, CH-1211 Geneva 23 (Switzerland); Baumann, P.; Didierjean, F.; Huck, A.; Knipper, A.; Ramdhane, M.; Walter, G. [CRN, CNRS-IN2P3, Universite Louis Pasteur, B.P. 28, F-67037 Strasbourg (France); Huyse, M.; Van Duppen, P. [Instituut voor Kern-en Stralingsfysica, University of Leuve n, Celestijnenlaan 200 D, B-3001 Leuven (Belgium); Marguier, G. [IPN, CNRS-IN2P3, Universite Claude Bernard, F-69622 Villeurb anne (France); Novikov, Y.; Popov, A.; Seliverstov, D.M. [St. Petersburg Nuclear Physics Institute, Gatchina, 188350 St. Pete rsburg, Russia] [CERN, CH-1211 Geneva 23 (Switzerland)


    {beta} decay of the T{sub z}={minus}1/2 nuclide {sup 71}Kr has been studied at the ISOLDE PSB Facility at CERN. {sup 71}Kr ions were produced in spallation reactions in a Nb foil using the 1 GeV proton beam and studied by means of {beta}-delayed proton, {beta}- and {gamma}-ray spectroscopy. The half-life and the {beta}-decay energy of {sup 71}Kr were determined using the decay of protons and positrons. These results: T{sub 1/2}=100{plus_minus}3ms and Q{sub EC}=10.14{plus_minus}0.32MeV and the first observation of the {beta} branch to the 207 keV level in {sup 71}Br makes the extension of the systematics of Gamow-Teller matrix elements of mirror nuclei up to A=71 possible. The Gamow-Teller strength of the same magnitude as that of the fp-shell mirror nuclei is observed for the ground-state transition. {copyright} {ital 1997} {ital The American Physical Society}

  7. Macrocyclic beta-sheet peptides that mimic protein quaternary structure through intermolecular beta-sheet interactions. (United States)

    Khakshoor, Omid; Demeler, Borries; Nowick, James S


    This paper reports the design, synthesis, and characterization of a family of cyclic peptides that mimic protein quaternary structure through beta-sheet interactions. These peptides are 54-membered-ring macrocycles comprising an extended heptapeptide beta-strand, two Hao beta-strand mimics [JACS 2000, 122, 7654] joined by one additional alpha-amino acid, and two delta-linked ornithine beta-turn mimics [JACS 2003, 125, 876]. Peptide 3a, as the representative of these cyclic peptides, contains a heptapeptide sequence (TSFTYTS) adapted from the dimerization interface of protein NuG2 [PDB ID: 1mio]. 1H NMR studies of aqueous solutions of peptide 3a show a partially folded monomer in slow exchange with a strongly folded oligomer. NOE studies clearly show that the peptide self-associates through edge-to-edge beta-sheet dimerization. Pulsed-field gradient (PFG) NMR diffusion coefficient measurements and analytical ultracentrifugation (AUC) studies establish that the oligomer is a tetramer. Collectively, these experiments suggest a model in which cyclic peptide 3a oligomerizes to form a dimer of beta-sheet dimers. In this tetrameric beta-sheet sandwich, the macrocyclic peptide 3a is folded to form a beta-sheet, the beta-sheet is dimerized through edge-to-edge interactions, and this dimer is further dimerized through hydrophobic face-to-face interactions involving the Phe and Tyr groups. Further studies of peptides 3b-3n, which are homologues of peptide 3a with 1-6 variations in the heptapeptide sequence, elucidate the importance of the heptapeptide sequence in the folding and oligomerization of this family of cyclic peptides. Studies of peptides 3b-3g show that aromatic residues across from Hao improve folding of the peptide, while studies of peptides 3h-3n indicate that hydrophobic residues at positions R3 and R5 of the heptapeptide sequence are important in oligomerization.

  8. Laser fabrication and spectroscopy of organic nanoparticles. (United States)

    Asahi, T; Sugiyama, T; Masuhara, H


    In working with nanoparticles, researchers still face two fundamental challenges: how to fabricate the nanoparticles with controlled size and shape and how to characterize them. In this Account, we describe recent advances in laser technology both for the synthesis of organic nanoparticles and for their analysis by single nanoparticle spectroscopy. Laser ablation of organic microcrystalline powders in a poor solvent has opened new horizons for the synthesis of nanoparticles because the powder sample is converted directly into a stable colloidal solution without additives and chemicals. By tuning laser wavelength, pulse width, laser fluence, and total shot number, we could control the size and phase of the nanoparticles. For example, we describe nanoparticle formation of quinacridone, a well-known red pigment, in water. By modifying the length of time that the sample is excited by the laser, we could control the particle size (30-120 nm) for nanosecond excitation down to 13 nm for femtosecond irradiation. We prepared beta- and gamma-phase nanoparticles from the microcrystal with beta-phase by changing laser wavelength and fluence. We present further results from nanoparticles produced from several dyes, C(60), and an anticancer drug. All the prepared colloidal solutions were transparent and highly dispersive. Such materials could be used for nanoscale device development and for biomedical and environmental applications. We also demonstrated the utility of single nanoparticle spectroscopic analysis in the characterization of organic nanoparticles. The optical properties of these organic nanoparticles depend on their size within the range from a few tens to a few hundred nanometers. We observed perylene nanoscrystals using single-particle spectroscopy coupled with atomic force microscopy. Based on these experiments, we proposed empirical equations explaining their size-dependent fluorescence spectra. We attribute the size effect to the change in elastic properties of

  9. beta-Scission of C-3 (beta-carbon) alkoxyl radicals on peptides and proteins

    DEFF Research Database (Denmark)

    Headlam, H A; Mortimer, A; Easton, C J


    of new reactive groups, including hydroperoxides. These processes can result in the loss of structural or enzymatic activity. Backbone fragmentation is known to occur via a number of mechanisms, most of which involve hydrogen abstraction from the alpha-carbon site on the backbone. In this study, we...... demonstrate that initial attack at a side chain site, the beta-position (C-3), can give rise to formation of alpha-carbon radicals, and hence backbone cleavage, via the formation and subsequent beta-scission of C-3 alkoxyl radicals. This beta-scission reaction is rapid (k estimated to be >10(7) s(-)(1)) even...

  10. Active Beam Spectroscopy (United States)

    von Hellermann, M. G.; Delabie, E.; Jaspers, R. J. E.; Biel, W.; Marchuk, O.; Summers, H. P.; Whiteford, A.; Giroud, C.; Hawkes, N. C.; Zastrow, K. D.


    Charge eXchange Recombination Spectroscopy (CXRS) plays a pivotal role in the diagnostics of hot fusion plasmas and is implemented currently in most of the operating devices. In the present report the main features of CXRS are summarized and supporting software packages encompassing "Spectral Analysis Code CXSFIT", "Charge Exchange Analysis Package CHEAP", and finally "Forward Prediction of Spectral Features" are described. Beam Emission Spectroscopy (BES) is proposed as indispensable cross-calibration tool for absolute local impurity density measurements and also for the continuous monitoring of the neutral beam power deposition profile. Finally, a full exploitation of the `Motional Stark Effect' pattern is proposed to deduce local pitch angles, total magnetic fields and possibly radial electric fields. For the proposed active beam spectroscopy diagnostic on ITER comprehensive performance studies have been carried out. Estimates of expected spectral signal-to-noise ratios are based on atomic modelling of neutral beam stopping and emissivities for CXRS, BES and background continuum radiation as well as extrapolations from present CXRS diagnostic systems on JET, Tore Supra, TEXTOR and ASDEX-UG. Supplementary to thermal features a further promising application of CXRS has been proposed recently for ITER, that is a study of slowing-down alpha particles in the energy range up to 2 MeV making use of the 100 keV/amu DNB (Diagnostic Neutral Beam) and the 500 keV/amu HNB (Heating Neutral Beam). Synthetic Fast Ion Slowing-Down spectra are evaluated in terms of source rates and slowing-down parameters

  11. Dark Matter Velocity Spectroscopy

    CERN Document Server

    Speckhard, Eric G; Beacom, John F; Laha, Ranjan


    Dark matter decays or annihilations that produce line-like spectra may be smoking-gun signals. However, even such distinctive signatures can be mimicked by astrophysical or instrumental causes. We show that velocity spectroscopy-the measurement of energy shifts induced by relative motion of source and observer-can separate these three causes with minimal theoretical uncertainties. The principal obstacle has been energy resolution, but upcoming and proposed experiments will make significant improvements. As an example, we show that the imminent Astro-H mission can use Milky Way observations to separate possible causes of the 3.5-keV line. We discuss other applications.

  12. Dark Matter Velocity Spectroscopy. (United States)

    Speckhard, Eric G; Ng, Kenny C Y; Beacom, John F; Laha, Ranjan


    Dark matter decays or annihilations that produce linelike spectra may be smoking-gun signals. However, even such distinctive signatures can be mimicked by astrophysical or instrumental causes. We show that velocity spectroscopy-the measurement of energy shifts induced by relative motion of source and observer-can separate these three causes with minimal theoretical uncertainties. The principal obstacle has been energy resolution, but upcoming experiments will have the precision needed. As an example, we show that the imminent Astro-H mission can use Milky Way observations to separate possible causes of the 3.5-keV line. We discuss other applications.

  13. Fourier transforms in spectroscopy

    CERN Document Server

    Kauppinen, Jyrki


    This modern approach to the subject is clearly and logically structured, and gives readers an understanding of the essence of Fourier transforms and their applications. All important aspects are included with respect to their use with optical spectroscopic data. Based on popular lectures, the authors provide the mathematical fundamentals and numerical applications which are essential in practical use. The main part of the book is dedicated to applications of FT in signal processing and spectroscopy, with IR and NIR, NMR and mass spectrometry dealt with both from a theoretical and practical poi

  14. Review on Hadron Spectroscopy

    CERN Document Server

    Liu, Chuan


    I review some of the lattice results on spectroscopy and resonances in the past years. For the conventional hadron spectrum computations, focus has been put on the isospin breaking effects, QED effects, and simulations near the physical pion mass point. I then go through several single-channel scattering studies within L\\"uscher formalism, a method that has matured over the past few years. The topics cover light mesons and also the charmed mesons, with the latter case intimately related to the recently discovered exotic $XYZ$ particles. Other possible related formalisms that are available on the market are also discussed.

  15. Nuclear Magnetic Resonance Spectroscopy (United States)


    devices same is (C22-C24). A spectrometer based on adc SQUID that is ,I suitable for NQR and low-frequency NMR spectroscopy has is been developed (C25...relatively few papers that saE is have a primarily instrumental focus. This is due in part to s the tendency of spectrometer and probe vendors not to publish...from NOE data, etc. sNoe i This has been reflected in two trends in data processing SEN0S 12 hardware. Spectrometer vendors are starting to move awav 9

  16. Strain-dependent differences in sensitivity of rat beta-cells to interleukin 1 beta in vitro and in vivo

    DEFF Research Database (Denmark)

    Reimers, J I; Andersen, H U; Mauricio, D


    The aim of this study was to investigate whether strain-dependent differences in beta-cell sensitivity to interleukin (IL) 1 beta exist in vitro and in vivo and if so, whether these differences correlate to variations in IL-1 beta-induced islet inducible nitric oxide synthase (iNOS) mRNA expression....../kg) or vehicle for 5 days. All the strains investigated were susceptible to IL-1 beta-induced changes in body weight, food intake, temperature, and plasma glucagon and corticosterone. However, IL-1 beta induced hyperglycemia and impairment of beta-cell glucose responsiveness in WK/Mol and LS/Mol rats...

  17. High-Resolution Conversion Electron Spectroscopy of Valence Electron Configurations (CESVEC) in Solids

    CERN Multimedia


    First measurements with the Zurich $\\beta$-spectrometer on sources from ISOLDE have demonstrated that high resolution spectroscopy of conversion electrons from valence shells is feasible.\\\\ \\\\ This makes possible a novel type of electron spectroscopy (CESVEC) on valence-electron configurations of tracer elements in solids. Thus the density of occupied electron states of impurities in solids has been measured for the first time. Such data constitute a stringent test of state-of-the-art calculations of impurity properties. Based on these results, we are conducting a systematic investigation of impurities in group IV and III-V semiconductors.

  18. Beta-Deacy Spectroscopy in the 100Sn Region:. Impact on Rp-Process Calculations (United States)

    Lorusso, G.; Becerril, A.; Amthor, A.; Baumann, T.; Bazin, D.; Berryman, J. S.; Brown, B. A.; Cyburt, R. H.; Crawford, H. L.; Estrade, A.; Gade, A.; Ginter, T.; Hausmann, C. M.; Mantica, P. F.; Matos, M.; Meharchand, R.; Minamisono, K.; Montes, F.; Perdikakis, G.; Pereira, J.; Portillo, M.; Schatz, H.; Smith, K.; Stoker, J.; Stolz, A.; Zegers, R. G. T.; Guess, C. J.; Hitt, G. W.


    -decay in the neighborhood of 100Sn was studied at the National Superconducting Cyclotron Laboratory (NSCL). The new half-lives of 96,97Cd, along with several new -delayed proton emission branching ratio for nuclei in this region have an impact on the composition of type-I X-ray burst ashes predicted by network calculations.

  19. A high-resolution spectroscopy survey of beta Cephei pulsations in bright stars

    NARCIS (Netherlands)

    Telting, J.H.; Schrijvers, C.; Ilyin, I.V.; Uytterhoeven, K.; Ridder, J. de; Aerts, C.C.; Henrichs, H.F.


    We present a study of absorption line-profile variations in early-B type near-main-sequence stars without emission lines. We have surveyed a total of 171 bright stars using the Nordic Optical Telescope (NOTSA), William Herschel Telescope (ING) and Coud�uxiliary Telescope (ESO). Our sample contains 7

  20. Hereditary and sporadic beta-amyloidoses. (United States)

    Di Fede, Giuseppe; Giaccone, Giorgio; Tagliavini, Fabrizio


    Cerebral amyloidoses are chronic, progressive neurodegenerative diseases that are caused by the aggregation and deposition of misfolded proteins in the central nervous system, and lead to cognitive deficits, stroke, and focal neurological dysfunction including cerebellar and extrapyramidal signs. Among them, beta-amyloidoses are a heterogenous set of conditions characterised by the deposition of beta-amyloid protein in brain parenchyma and/or vessel walls that lead to the development of two main clinico-pathological entities: Alzheimer's disease and cerebral amyloid angiopathy, which may be sporadic or familial, and may also co-exist in the same patient. The aim of this review is to describe the most important differences in the pathways leading to parenchymal and cerebrovascular beta-amyloidoses, and the main clinical, neuropathological and biochemical characteristics of the two conditions. It also discusses the phenotypes associated with a series of familial and sporadic beta-amyloidoses in more detail in order to highlight the clinical and neuropathological features that may help to distinguish the different forms of disease.

  1. beta-decay of O-13

    NARCIS (Netherlands)

    Knudsen, HH; Fynbo, HOU; Borge, MJG; Boutami, R; Dendooven, P; Diget, CA; Eronen, T; Fox, S; Fraile, LM; Fulton, B; Huikary, J; Jeppesen, HB; Jokinen, AS; Jonson, B; Kankainen, A; Moore, [No Value; Nieminen, A; Nyman, G; Penttila, H; Riisager, K; Rinta-Antila, S; Tengblad, O; Wang, Y; Wilhelmsen, K; Aysto, J


    The beta decay of O-13 has been studied at the IGISOL facility of the Jyvaskyla accelerator centre (Finland). By developing a low-energy isotope-separated beam of O-13 and using a modern segmented charged-particle detector array an improved measurement of the delayed proton spectrum was possible. Pr

  2. Characterization of a Commercial Silicon Beta Cell

    Energy Technology Data Exchange (ETDEWEB)

    Foxe, Michael P. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Hayes, James C. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Mayer, Michael F. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); McIntyre, Justin I. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Sivels, Ciara B. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Suarez, Rey [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)


    Silicon detectors are of interest for the verification of the Comprehensive Nuclear-Test-Ban Treaty (CTBT) due to their enhanced energy resolution compared to plastic scintillators beta cells. Previous work developing a figure-of-merit (FOM) for comparison of beta cells suggests that the minimum detectable activity (MDA) could be reduced by a factor of two to three with the use of silicon detectors. Silicon beta cells have been developed by CEA (France) and Lares Ltd. (Russia), with the PIPSBox developed by CEA being commercially available from Canberra for approximately $35k, but there is still uncertainty about the reproducibility of the capabilities in the field. PNNL is developing a high-resolution beta-gamma detector system in the shallow underground laboratory, which will utilize and characterize the operation of the PIPSBox detector. Throughout this report, we examine the capabilities of the PIPSBox as developed by CEA. The lessons learned through the testing and use of the PIPSBox will allow PNNL to strategically develop a silicon detector optimized to better suit the communities needs in the future.

  3. Association behavior of native beta-lactoglobulin

    DEFF Research Database (Denmark)

    Verheul, M.; Pedersen, J.S.; Roefs, S.P.F.M.


    The association behavior of beta-lactoglobulin has been studied by small-angle neutron scattering as a function of protein concentration, temperature, pH, and NaCl concentration of the solution. By indirect Fourier transformation of the spectra, pair-distance distribution functions for the variou...

  4. Poster: The EURISOL Beta-beam facility

    CERN Document Server

    The beta-beam concept for the generation of an electron (anti-)neutrino beam was proposed by Piero Zucchelli (CERN) in 2002. A first study of the possibility of using the existing CERN machines for the acceleration for radioactive ions to a relativistic gamma of roughly 100, for later storage in a new decay ring of approximately the size of SPS, was made in 2002. The results from this very first short study were very encouraging.In 2004 it was decided to incorporate a design study for the beta-beam within the EURISOL DS proposal. EURISOL is a project name for a next-generation radioactive beam facility based on the ISOL method for the production of intense radioactive beams for nuclear physics, astrophysics and other applications. The proposal was accepted with the beta-beam task as an integral part. The design study officially started 1 February 2005 and will run for 4 years resulting in a conceptual design report for a beta-beam facility.

  5. Graphene oxide strongly inhibits amyloid beta fibrillation

    NARCIS (Netherlands)

    Mahmoudi, Morteza; Akhavan, Omid; Ghavami, Mahdi; Rezaee, Farhad; Ghiasi, Seyyed Mohammad Amin


    Since amyloid beta fibrillation (AbF) plays an important role in the development of neurodegenerative diseases, we investigated the effect of graphene oxide (GO) and their protein-coated surfaces on the kinetics of Ab fibrillation in the aqueous solution. We showed that GO and their protein-covered

  6. Topical beta-Blockers and Mortality

    NARCIS (Netherlands)

    Muskens, Rogier P. H. M.; Wolfs, Roger C. W.; Wittenian, Jacqueline C. M.; Hofman, Albert; de Jong, Paulus T. V. M.; Stricker, Bruno H. C.; Jansonius, Nomdo M.


    Purpose: To study the associations between long-term and short-term use of topical beta-blockers and mortality. Design: Prospective population-based cohort study. Participants: To examine long-term effects, 3842 participants aged 55 years and older were recruited. To examine short-term effects, 484

  7. Tests of fundamental symmetries with beta decay

    NARCIS (Netherlands)

    vanKlinken, J


    Since the fall of parity in electroweak interactions the discrete transformations of parity, charge conjugation and time reversal are under close scrutiny for any sign of deviation from maximality where symmetry breaking seems to be complete (like P violation in beta decay) and for any sign of symme

  8. Beta Bremsstrahlung dose in concrete shielding (United States)

    Manjunatha, H. C.; Chandrika, B. M.; Rudraswamy, B.; Sankarshan, B. M.


    In a nuclear reactor, beta nuclides are released during nuclear reactions. These betas interact with shielding concrete and produces external Bremsstrahlung (EB) radiation. To estimate Bremsstrahlung dose and shield efficiency in concrete, it is essential to know Bremsstrahlung distribution or spectra. The present work formulated a new method to evaluate the EB spectrum and hence Bremsstrahlung dose of beta nuclides (32P, 89Sr, 90Sr-90Y, 90Y, 91Y, 208Tl, 210Bi, 234Pa and 40K) in concrete. The Bremsstrahlung yield of these beta nuclides in concrete is also estimated. The Bremsstrahlung yield in concrete due to 90Sr-90Y is higher than those of other given nuclides. This estimated spectrum is accurate because it is based on more accurate modified atomic number (Zmod) and Seltzer's data, where an electron-electron interaction is also included. Presented data in concrete provide a quick and convenient reference for radiation protection. The present methodology can be used to calculate the Bremsstrahlung dose in nuclear shielding materials. It can be quickly employed to give a first pass dose estimate prior to a more detailed experimental study.

  9. Alpha and Beta at the B Factories

    Energy Technology Data Exchange (ETDEWEB)

    Finocchiaro, Giuseppe; /Frascati


    We review recent experimental results on time-dependent CP asymmetries in the B system from the BABAR and Belle experiments. Measurements of the {alpha} and {beta} angles of the Unitarity Triangle of the CKM matrix are discussed. These measurements constitute stringent tests of the Standard Model, and are also used to search for new physics.

  10. Size-dependent neurotoxicity of beta-amyloid oligomers. (United States)

    Cizas, Paulius; Budvytyte, Rima; Morkuniene, Ramune; Moldovan, Radu; Broccio, Matteo; Lösche, Mathias; Niaura, Gediminas; Valincius, Gintaras; Borutaite, Vilmante


    The link between the size of soluble amyloid beta (Abeta) oligomers and their toxicity to rat cerebellar granule cells (CGC) was investigated. Variation in conditions during in vitro oligomerization of Abeta(1-42) resulted in peptide assemblies with different particle size as measured by atomic force microscopy and confirmed by dynamic light scattering and fluorescence correlation spectroscopy. Small oligomers of Abeta(1-42) with a mean particle z-height of 1-2 nm exhibited propensity to bind to phospholipid vesicles and they were the most toxic species that induced rapid neuronal necrosis at submicromolar concentrations whereas the bigger aggregates (z-height above 4-5 nm) did not bind vesicles and did not cause detectable neuronal death. A similar neurotoxic pattern was also observed in primary cultures of cortex neurons whereas Abeta(1-42) oligomers, monomers and fibrils were non-toxic to glial cells in CGC cultures or macrophage J774 cells. However, both oligomeric forms of Abeta(1-42) induced reduction of neuronal cell densities in the CGC cultures.

  11. Synthesis of magnetite nanoparticles-{beta}-cyclodextrin complex

    Energy Technology Data Exchange (ETDEWEB)

    Cobos Cruz, L.A.; Martinez Perez, C.A. [Instituto de Ingenieria y Tecnologia, Universidad Autonoma de Cd. Juarez, Ave. del Charro 450, Col Partido Romero, C.P. 32360, Cd. Juarez Chih. (Mexico); Monreal Romero, H.A. [Facultad de Odontologia, Universidad Autonoma de Chihuahua, Ciudad Universitaria Campus I, C.P. 31000, Chihuahua, Chi. Mexico (Mexico); Garcia Casillas, P.E. [Instituto de Ingenieria y Tecnologia, Universidad Autonoma de Cd. Juarez, Ave. del Charro 450, Col Partido Romero, C.P. 32360, Cd. Juarez Chih. (Mexico)], E-mail:


    In this work, the synthesis and characterization of a magnetite (M) and {beta}-cyclodextrin (CD) complex is presented. The chemical bonding between the magnetite and CD was studied as evidence of host-guest interaction; therefore the CD works like a reactor with the magnetite inside of it, as consequence the growth of the particle is restricted by the electrostatic interaction of M-CD complex. The particle size of the magnetite-cyclodextrin complex (M-CD) decreased 79.1% with 0.5% of CD. The average particle size of the M-CD complex was 10 nm. The saturation magnetization ({sigma}{sub s}) and intrinsic coercivity (H{sub c}) increased 10% and 20%, respectively. In order to understand how the the CD affects the results obtained, the second derivate of remission function was obtained from the ultraviolet-visible spectra (UV-vis). Fourier transform infrared spectroscopy (FTIR) was used to elucidate the interaction between the magnetite and CD. The thermal analysis was measured by thermogravimetric and differential thermal analysis (TGA-DTA). The magnetic properties, intrinsic coercivity (H{sub c}) and the saturation magnetization were determined by vibrating sample magnetometry (VSM); the size and shape of nanoparticles were determined by scanning electron microscopy (SEM). The identification of phases was made by X-ray diffraction.

  12. Herschel detects oxygen in the beta Pictoris debris disk

    CERN Document Server

    Brandeker, A; Olofsson, G; Vandenbussche, B; Acke, B; Barlow, M J; Blommaert, J A D L; Cohen, M; Dent, W R F; Dominik, C; Di Francesco, J; Fridlund, M; Gear, W K; Glauser, A M; Greaves, J S; Harvey, P M; Heras, A M; Hogerheijde, M R; Holland, W S; Huygen, R; Ivison, R J; Leeks, S J; Lim, T L; Liseau, R; Matthews, B C; Pantin, E; Pilbratt, G L; Royer, P; Sibthorpe, B; Waelkens, C; Walker, H J


    The young star beta Pictoris is well known for its dusty debris disk, produced through the grinding down by collisions of planetesimals, kilometre-sized bodies in orbit around the star. In addition to dust, small amounts of gas are also known to orbit the star, likely the result from vaporisation of violently colliding dust grains. The disk is seen edge on and from previous absorption spectroscopy we know that the gas is very rich in carbon relative to other elements. The oxygen content has been more difficult to assess, however, with early estimates finding very little oxygen in the gas at a C/O ratio 20x higher than the cosmic value. A C/O ratio that high is difficult to explain and would have far-reaching consequences for planet formation. Here we report on observations by the far-infrared space telescope Herschel, using PACS, of emission lines from ionised carbon and neutral oxygen. The detected emission from C+ is consistent with that previously reported being observed by the HIFI instrument on Herschel,...

  13. Neutron resonance spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Gunsing, F


    The present document has been written in order to obtain the diploma 'Habilitation a Diriger des Recherches'. Since this diploma is indispensable to supervise thesis students, I had the intention to write a document that can be useful for someone starting in the field of neutron resonance spectroscopy. Although the here described topics are already described elsewhere, and often in more detail, it seemed useful to have most of the relevant information in a single document. A general introduction places the topic of neutron-nucleus interaction in a nuclear physics context. The large variations of several orders of magnitude in neutron-induced reaction cross sections are explained in terms of nuclear level excitations. The random character of the resonances make nuclear model calculation predictions impossible. Then several fields in physics where neutron-induced reactions are important and to which I have contributed in some way or another, are mentioned in a first synthetic chapter. They concern topics like parity nonconservation in certain neutron resonances, stellar nucleosynthesis by neutron capture, and data for nuclear energy applications. The latter item is especially important for the transmutation of nuclear waste and for alternative fuel cycles. Nuclear data libraries are also briefly mentioned. A second chapter details the R-matrix theory. This formalism is the foundation of the description of the neutron-nucleus interaction and is present in all fields of neutron resonance spectroscopy. (author)

  14. Wave mixing spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Smith, R.W.


    Several new aspects of nonlinear or wave mixing spectroscopy were investigated utilizing the polarization properties of the nonlinear output field and the dependence of this field upon the occurrence of multiple resonances in the nonlinear susceptibility. First, it is shown theoretically that polarization-sensitive detection may be used to either eliminate or controllably reduce the nonresonant background in coherent anti-Stokes Raman spectroscopy, allowing weaker Raman resonances to be studied. The features of multi-resonant four-wave mixing are examined in the case of an inhomogeneously broadened medium. It is found that the linewidth of the nonlinear output narrows considerably (approaching the homogeneous width) when the quantum mechanical expressions for the doubly- and triply-resonant susceptibilities are averaged over a Doppler or strain broadened profile. Experimental studies of nonlinear processes in Pr/sup +3/:LaF/sub 3/ verify this linewidth narrowing, but indicate that this strain broadened system cannot be treated with a single broadening parameter as in the case of Doppler broadening in a gas. Several susceptibilities are measured from which are deduced dipole matrix elements and Raman polarizabilities related to the /sup 3/H/sub 4/, /sup 3/H/sub 6/, and /sup 3/P/sub 0/ levels of the praseodymium ions.

  15. Universality in Chiral Random Matrix Theory at $\\beta =1$ and $\\beta =4$

    CERN Document Server

    Sener, M K


    In this paper the kernel for spectral correlation functions of the invariant chiral random matrix ensembles with real ($\\beta =1$) and quaternion real ($\\beta = 4$) matrix elements is expressed in terms of the kernel of the corresponding complex Hermitean random matrix ensembles ($\\beta=2$). Such identities are exact in case of a Gaussian probability distribution and, under certain smoothness assumptions, they are shown to be valid asymptotically for an arbitrary finite polynomial potential. They are proved by means of a construction proposed by Brézin and Neuberger. Microscopic universality for all three chiral ensembles then follows from universal behavior for $\\beta =2$ both at the hard edge (shown by Akemann, Damgaard, Magnea and Nishigaki) and at the soft edge of the spectrum (shown by Kanzieper and Freilikher).


    Directory of Open Access Journals (Sweden)

    Ante Perković


    Full Text Available The capital asset pricing model (CAPM is one of the most important models in financial economics and it has a long history of theoretical and empirical investigations. The main underlying concept of the CAPM model is that assets with a high risk (high beta should earn a higher return than assets with a low risk (low beta and vice versa. The implication which can be drawn out of this is that all assets with a beta above zero bear some risk and therefore their expected return is above the return of the risk-free rate. In this research observation on monthly stock prices on Croatian stock market from January 1st 2005 until December 31st 2009 is used to form our sample. CROBEX index is used as proxy of the market portfolio. The results demonstrate that beta can not be trusted in making investment decisions and rejects the validity of the whole CAPM model on Croatian stock market.

  17. The X-Array and SATURN: A new decay-spectroscopy station for CARIBU

    CERN Document Server

    Mitchell, A J; DiGiovine, B; Lister, C J; Carpenter, M P; Chowdhury, P; Clark, J A; D'Olympia, N; Deo, A Y; Kondev, F G; McCutchan, E A; Rohrer, J; Savard, G; Seweryniak, D; Zhu, S


    A new decay-spectroscopy station has been commissioned for experiments with low-energy, fission-fragment radioactive beams from the CARIBU ion source. The new set-up consists of the 'X-array', a highly-efficient array of HPGe clover detectors, and 'SATURN' (Scintillator And Tape Using Radioactive Nuclei), a plastic scintillator detector combined with a tape-transport system for detection of beta particles and removal of long-lived isobaric decay products.

  18. Interleukin-1 beta (IL-1 beta) induces thrombocytosis in mice: Possible implication of IL-6

    Energy Technology Data Exchange (ETDEWEB)

    Kimura, H.; Ishibashi, T.; Shikama, Y.; Okano, A.; Akiyama, Y.; Uchida, T.; Maruyama, Y. (Fukushima Medical College (Japan))


    We administered recombinant human interleukin-1 beta (IL-1 beta), the common mediator of inflammation process, to C57B1/6 male mice (0.5 microgram, every 12 hours over five times) intraperitoneally and consequently induced a remarkable thrombocytosis. Day 1 was designated as the following day of the last injection in the morning. A significant thrombocytosis was observed on days 1 through 5 with a peak on day 2 (162 +/- 9 x 10(4)/mm3) compared with the control mice injected with heated IL-1 beta (101 +/- 11 x 10(4)/mm3). A striking increase in mean size of marrow megakaryocytes was noted on days 1 and 2. The incorporation of 75Se-selenomethionine into circulating platelets as a measure of platelet production was about 2.3 times higher in IL-1 beta-treated mice than in control mice. To determine which factor(s) is responsible for elicited thrombocytosis, the in vitro studies and bioassays for several hematopoietic factors were performed. IL-1 beta by itself did not stimulate megakaryocytopoiesis in vitro, suggesting that the thrombocytosis is attributed to other factor(s) via IL-1 beta stimulation. Serum colony-stimulating factor (CSF) activity after a single IL-1 beta (0.5 microgram) injection, monitored by colony assay with 10% tested serum, peaked at 3 hours. Formed colonies were mostly granulocyte (G) and granulocyte-macrophage (GM)-types, and studies using rabbit anti-mouse GM-CSF serum or using human marrow as target cells showed that the CSF activity of the tested serum consisted of, at least, GM-CSF and G-CSF. Addition of IL-3 concomitantly with the tested serum gave rise to a greater number of megakaryocytic colonies. Serum IL-3, monitored by IL-3-dependent cell line 32D clone 5, and erythropoietin activities were not detected at serum level in IL-1 beta-treated mice.

  19. Vibrational spectroscopy at electrified interfaces

    CERN Document Server

    Wieckowski, Andrzej; Braunschweig, Björn


    Reviews the latest theory, techniques, and applications Surface vibrational spectroscopy techniques probe the structure and composition of interfaces at the molecular level. Their versatility, coupled with their non-destructive nature, enables in-situ measurements of operating devices and the monitoring of interface-controlled processes under reactive conditions. Vibrational Spectroscopy at Electrified Interfaces explores new and emerging applications of Raman, infrared, and non-linear optical spectroscopy for the study of charged interfaces. The book draws from hu

  20. Array-based photoacoustic spectroscopy (United States)

    Autrey, S. Thomas; Posakony, Gerald J.; Chen, Yu


    Methods and apparatus for simultaneous or sequential, rapid analysis of multiple samples by photoacoustic spectroscopy are disclosed. A photoacoustic spectroscopy sample array including a body having at least three recesses or affinity masses connected thereto is used in conjunction with a photoacoustic spectroscopy system. At least one acoustic detector is positioned near the recesses or affinity masses for detection of acoustic waves emitted from species of interest within the recesses or affinity masses.

  1. Effect of heating rate on temperature of titanium alloy (. cap alpha. +. beta. ). -->. beta. transformaton

    Energy Technology Data Exchange (ETDEWEB)

    Gridnev, V.N.; Ivasishin, O.M.; Markovskij, P.E. (AN Ukrainskoj SSR, Kiev. Inst. Metallofiziki)


    The effect of doping of two-phase titaniums alloys and morphology of initial structure on the Tsub(t) temperature shift value of (..cap alpha..+..beta..)..--> beta.. transformation depending on heating rate is investigated. It has been found that the Tsub(t) shift occurs in the strictly determined temperature range depending on chemical alloy composition. The Tsub(t) shift is directly proportional to the Ksub(..beta..) coefficient applied as a quantitative alloying characteristic as well as a dimensional factor equal either to the plate thickness or the ..cap alpha..-phase globule diameter depending on the type of initial structure. In the limits of this temperature range the (..cap alpha..+..beta..)..--> beta..-transformation occurs completely according to the diffusion mechanism. The critical heating rate at which maximum permissible Tsub(t) value is attained and above which its stabilization is observed is determined by the same parameters - the alloy doping degree characterized by the Ksub(..beta..) coefficient and the ..cap alpha..-phase crystal dimensions in the initial structure.

  2. Photoelectron photoion molecular beam spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Trevor, D.J.


    The use of supersonic molecular beams in photoionization mass spectroscopy and photoelectron spectroscopy to assist in the understanding of photoexcitation in the vacuum ultraviolet is described. Rotational relaxation and condensation due to supersonic expansion were shown to offer new possibilities for molecular photoionization studies. Molecular beam photoionization mass spectroscopy has been extended above 21 eV photon energy by the use of Stanford Synchrotron Radiation Laboratory (SSRL) facilities. Design considerations are discussed that have advanced the state-of-the-art in high resolution vuv photoelectron spectroscopy. To extend gas-phase studies to 160 eV photon energy, a windowless vuv-xuv beam line design is proposed.

  3. Bridged bis(beta-cyclodextrin)s possessing coordinated metal center(s) and their inclusion complexation behavior with model substrates: enhanced molecular binding ability by multiple recognition. (United States)

    Liu, Y; Chen, Y; Li, L; Zhang, H Y; Liu, S X; Guan, X D


    To investigate quantitatively the cooperative binding ability of several beta-cyclodextrin oligomers bearing single or multiligated metal center(s), the inclusion complexation behavior of four bis(beta-cyclodextrin)s (2-5) linked by 2,2'-bipyridine-4,4'-dicarboxy tethers and their copper(II) complexes (6-9) with representative dye guests, i.e., methyl orange (MO), acridine red (AR), rhodamine B (RhB), ammonium 8-anilino-1-naphthalenesulfonic acid (ANS), and sodium 6-(p-toludino)-2-naphthalenesulfonate (TNS), have been examined in aqueous solution at 25 degrees C by means of UV-vis, circular dichroism, fluorescence, and 2D NMR spectroscopy. The results obtained indicate that bis(beta-cyclodextrin)s 2-5 can associate with one or three copper(II) ion(s) producing 2:1 or 2:3 bis(beta-cyclodextrin)-copper(II) complexes. These metal-ligated oligo(beta-cyclodextrin)s can bind two model substrates to form intramolecular 2:2 host-guest inclusion complexes and thus significantly enhance the original binding abilities of parent beta-cyclodextrin and bis(beta-cyclodextrin) toward model substrates through the cooperative binding of two guest molecules by four tethered cyclodextrin moieties, as well as the additional binding effect supplied by ligated metal center(s). Host 6 showed the highest enhancement of the stability constant, up to 38.3 times for ANS as compared with parent beta-cyclodextrin. The molecular binding mode and stability constant of substrates by bridged bis- and oligo(beta-cyclodextrin)s 2-9 are discussed from the viewpoint of the size/shape-fit interaction and molecular multiple recognition between host and guest.

  4. TGF-beta and BMP in breast cancer cell invasion

    NARCIS (Netherlands)

    Naber, Hildegonda Petronella Henriëtte


    TGF-beta and BMPs are members of the TGF-beta superfamily of cytokines which play an important role in a multitude of processes. In cancer, TGF-beta is known for its dual role: in early stages it inhibits cancer cell proliferation, whereas in later stages it promotes invasion and metastasis. In this

  5. First forbidden beta decay of some strongly deformed nuclei

    NARCIS (Netherlands)

    Werf, Siebren Ysbrand van der


    In this thesis we present measurements of the shape factors of the first forbidden beta decays of the nuclei Tm^170, Re^186, Re^188 and Lu^176m. For Lu^176m, also the beta gamma directionalcorrelation was measured. In chapter I formulas for the observable quantities in first forbidden beta decay and

  6. Partial beta-amylolysis retards starch retrogradation in rice products. (United States)

    Yao, Yuan; Zhang, Jingmin; Ding, Xiaolin


    Starch retrogradation is the main cause of quality deterioration of starch-containing foods during storage. The current work investigated the effect of partial beta-amylolysis on the retrogradation of rice starch and the potential of beta-amylase in preparing rice products with extended shelf life. Isolated amylopectin, whole rice starch, and rice flour from a regular rice cultivar were partially hydrolyzed by either reagent-grade or food-grade beta-amylase. The degree of beta-amylolysis was expressed as average external chain length () for isolated amylopectin or the degree of hydrolysis (%) for other starch systems. Pulsed nuclear magnetic resonance was used to monitor starch retrogradation during storage at 4 degrees C. The results indicated that partial beta-amylolysis using reagent-grade beta-amylase retarded amylopectin retrogradation by shortening the of amylopectin. When was below DP 11.6, the amylopectin retrogradation was essentially inhibited. Partial beta-amylolysis had a similar effect on the amylopectin retrogradation in the whole starch system. The maltose produced in beta-amylolysis might slightly attenuate the retrogradation-retarding effect of partial beta-amylolysis. The effect of food-grade beta-amylase on starch retrogradation was also evident, although less effective than that of reagent-grade beta-amylase. The retrogradation-retarding effect of food-grade beta-amylase was also demonstrated in rice flour system, indicating a potential method for controlling the starch retrogradation of rice products.

  7. Estimating security betas using prior information based on firm fundamentals

    NARCIS (Netherlands)

    Cosemans, Mathijs; Frehen, Rik; Schotman, Peter; Bauer, Rob


    We propose a hybrid approach for estimating beta that shrinks rolling window estimates towards firm-specific priors motivated by economic theory. Our method yields superior forecasts of beta that have important practical implications. First, hybrid betas carry a significant price of risk in the cros

  8. Mechanisms of beta-amyloid neurotoxicity : Perspectives of pharmacotherapy

    NARCIS (Netherlands)

    Harkany, T; Abraham, [No Value; Konya, C; Nyakas, C; Zarandi, M; Penke, B; Luiten, PGM


    One of the characteristic neuropathological hallmarks of Alzheimer's disease (AD) is the extracellular accumulation of beta -amyloid peptides (A beta) in neuritic plaques, Experimental data indicate that different molecular forms of A beta affect a wide array of neuronal and glial functions and ther

  9. Comparative seric TGF({beta}1, {beta}2) levels and platelets count response in total body irradiated baboons; Evolution comparee des taux seriques des TGF ({beta}1, {beta}2) et de la numeration plaquettaire chez le babouin irradie globalement

    Energy Technology Data Exchange (ETDEWEB)

    Mestries, J.C.; Veyret, J.; Agay, D.; Van Uye, A.; Caterini, R.; Herodin, F.; Mathieu, J.; Chancerelle, Y.


    Total body irradiation associated or not with r-hIL-6 treatment a relation between TGF-{beta}1 and TGF-{beta}2 blood levels and platelets count. During radio-induced thrombocytopenia, by decreasing its ability to inhibit proliferation of stem cells and megakaryocytopoiesis, the TGF-{beta} falling induced a favorable condition for hematopoietic recovery. (author). 5 refs.

  10. Induction of beta-lactamase production in Pseudomonas aeruginosa biofilm

    DEFF Research Database (Denmark)

    Giwercman, B; Jensen, E T; Høiby, N;


    Imipenem induced high levels of beta-lactamase production in Pseudomonas aeruginosa biofilms. Piperacillin also induced beta-lactamase production in these biofilms but to a lesser degree. The combination of beta-lactamase production with other protective properties of the biofilm mode of growth...

  11. Isolation and characterization of chicken and turkey beta 2-microglobulin

    DEFF Research Database (Denmark)

    Skjødt, K; Welinder, K G; Crone, M


    Chicken and turkey beta 2-m were isolated from citrated plasma in sequential use of three chromatographic steps: affinity chromatography, gel filtration chromatography and anion-exchange chromatography. The purified protein was identified as beta 2-m by reaction with a beta 2-m specific monoclonal...

  12. Stereoselective synthesis of nicotinamide beta-riboside and nucleoside analogs. (United States)

    Franchetti, Palmarisa; Pasqualini, Michela; Petrelli, Riccardo; Ricciutelli, Massimo; Vita, Patrizia; Cappellacci, Loredana


    The beta-anomers of N-ribofuranosylnicotine-3-carboxamide (beta-NAR) and its nicotinic acid analog (beta-NaR) were obtained by stereoselective synthesis via glycosylation of the presilylated bases under Vorbruggen's protocol. A NAR analog, methylated in position 3 of the ribosylic moiety, is also reported.

  13. Right tail expansion of Tracy-Widom beta laws

    CERN Document Server

    Borot, Gaëtan


    Using loop equations, we compute the large deviation function of the maximum eigenvalue to the right of the spectrum in the Gaussian beta matrix ensembles, to all orders in 1/N. We then give a physical derivation of the all order asymptotic expansion of the right tail Tracy-Widom beta laws, for all positive beta, by studying the double scaling limit.

  14. {beta}-Carotene to bacteriochlorophyll c energy transfer in self-assembled aggregates mimicking chlorosomes

    Energy Technology Data Exchange (ETDEWEB)

    Alster, J. [Faculty of Mathematics and Physics, Charles University, Ke Karlovu 3, 121 16 Praha (Czech Republic); Polivka, T. [Institute of Physical Biology, University of South Bohemia, Zamek 136, 373 33 Nove Hrady (Czech Republic); Biology Centre, Academy of Sciences of the Czech Republic, Branisovska 31, 370 05 Ceske Budejovice (Czech Republic); Arellano, J.B. [Instituto de Recursos Naturales y Agrobiologia de Salamanca (IRNASA-CSIC), Apdo. 257, 37071 Salamanca (Spain); Chabera, P. [Institute of Physical Biology, University of South Bohemia, Zamek 136, 373 33 Nove Hrady (Czech Republic); Vacha, F. [Institute of Physical Biology, University of South Bohemia, Zamek 136, 373 33 Nove Hrady (Czech Republic); Biology Centre, Academy of Sciences of the Czech Republic, Branisovska 31, 370 05 Ceske Budejovice (Czech Republic); Psencik, J., E-mail: [Faculty of Mathematics and Physics, Charles University, Ke Karlovu 3, 121 16 Praha (Czech Republic); Institute of Physical Biology, University of South Bohemia, Zamek 136, 373 33 Nove Hrady (Czech Republic)


    Carotenoids are together with bacteriochlorophylls important constituents of chlorosomes, the light-harvesting antennae of green photosynthetic bacteria. Majority of bacteriochlorophyll molecules form self-assembling aggregates inside the chlorosomes. Aggregates of bacteriochlorophylls with optical properties similar to those of chlorosomes can also be prepared in non-polar organic solvents or in aqueous environments when a suitable non-polar molecule is added. In this work, the ability of {beta}-carotene to induce aggregation of bacteriochlorophyll c in aqueous buffer was studied. Excitation relaxation and energy transfer in the carotenoid-bacteriochlorophyll assemblies were measured using femtosecond and nanosecond transient absorption spectroscopy. A fast, {approx}100-fs energy transfer from the S{sub 2} state of {beta}-carotene to bacteriochlorophyll c was revealed, while no evidence for significant energy transfer from the S{sub 1} state was found. Picosecond formation of the carotenoid triplet state (T{sub 1}) was observed, which was likely generated by singlet homo-fission from the S{sub 1} state of {beta}-carotene.

  15. Preparation of hydroxyapatite nanoparticles facilitated by the presence of {beta}-cyclodextrin

    Energy Technology Data Exchange (ETDEWEB)

    Martinez-Perez, Carlos A., E-mail: [Institute of Engineering and Technology, Autonomous University of Juarez, UACJ, Ave. del Charro 610 norte, C.P. 32320, Cd. Juarez, Chihuahua (Mexico); Garcia-Montelongo, Jorge; Garcia Casillas, Perla E.; Farias-Mancilla, Jose R. [Institute of Engineering and Technology, Autonomous University of Juarez, UACJ, Ave. del Charro 610 norte, C.P. 32320, Cd. Juarez, Chihuahua (Mexico); Monreal Romero, Humberto [School of Odontology, Autonomous University of Chihuahua, UACH, Ave. Universidad s/n Campus Universitario I, C.P. 31170, Chihuahua (Mexico)


    Highlights: Black-Right-Pointing-Pointer It was found that {beta}-cyclodextrin can control the particle size in the production of nanohydroxyapatite. Black-Right-Pointing-Pointer Particle size in the range of 30-50 nm was obtained. Black-Right-Pointing-Pointer A new simple methodology for the preparation of hydroxyapatite nanoparticles with a well controlled size and narrow particles size distribution was developed. - Abstract: Hydroxyapatite nanoparticles with uniform morphology have been successfully synthesized by a chemical coprecipitation method and facilitated by the presence of the {beta}-cyclodextrin. X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM); and Fourier Transformed Infrared Spectroscopy (FT-IR) were used in order to characterize the hydroxyapatite samples. The experimental results indicate that the obtained HA is in the range of 20-50 nm. Also it was found that the content of {beta}-CD has an impact on the purity of the HA as well in the particle size of the hydroxyapatite nanoparticles.

  16. [The preparation, characterization and ultraviolet photodegradation of LNG-HP-beta-CD]. (United States)

    Wang, Da-wei; Liu, Qi; Liu, Ming; Liu, Xiao-hui


    The characteristics of levonorgestrel (LNG), low solubility and the quick degradation under ultraviolet, limited its study and application in rodent contraception. The inclusion complex of hydroxypropyl-beta-cyclodextrin (HP-beta-CD) with LNG was investigated in present study. The inclusion complex was prepared by solution method and characterized by ultraviolet absorption spectrum and infrared spectrum spectra. And the stability was evaluated by being exposed to ultraviolet. The authors' results showed that the accurate and simple method of quantitative determination for LNG was established by ultraviolet spectrum, the molar ratio of the complex was 1:1 calculated from the phase solubility diagram, the stability constant was 187.3 L x mol(-1) at 25 degrees C, and the formation of the inclusion complex was validated by UV-Vis and Fourier transform infrared spectroscopy. Moreover, the degradation rate of the inclusion complex was less than 5%, which was slower than the LNG monomer. The present study indicated that HP-beta-CD could be formed inclusion complexes with LNG and the solubility, and stability were obviously enhanced.

  17. Synthesis of a metal binding protein designed on the alpha/beta scaffold of charybdotoxin. (United States)

    Pierret, B; Virelizier, H; Vita, C


    The alpha/beta scaffold of the scorpion toxin charybdotoxin has been used for the engineering of a metal binding site. Nine substitutions, including three histidines as metal ligands, have been introduced into the original toxin sequence. The newly designed sequence, 37 amino acids long, has been assembled by solid-phase synthesis and HBTU (2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) coupling of Fmoc-protected amino acids. Formation of the three disulfide bonds occurred efficiently and rapidly in the presence of glutathione, and this post-synthesis modification has facilitated the purification task enormously. The process of synthesis and purification was performed in less than a week with an overall 10.2% yield. Circular dichroism analysis showed that the newly designed protein is folded in a alpha/beta structure, similarly to the parent toxin. Electronic absorption spectroscopy, circular dichroism and gel filtration experiments have been used to show that Cu2+ and Zn2+ ions bind with high affinity to the newly engineered protein. These results demonstrate that the alpha/beta fold, common to all scorpion toxins, is a very versatile basic structure, tolerant for substitutions and able to present new sequences in a predetermined conformation. The chemical approach is shown to be effective, rapid and practical for the production of novel designed small proteins.

  18. Periodic Radio Continuum Emission Associated with the beta Cephei Star V2187 Cyg

    CERN Document Server

    Tapia, Mauricio; Tovmassian, Gagik; Rodriguez-Gomez, Vicente; Gonzalez-Buitrago, Diego; Zharikov, Sergei; Ortiz-Leon, Gisela N


    We present new optical time-resolved photometry and medium-resolution spectroscopy of V2187 Cyg. We confirm its classification as a beta Cephei star based on sinusoidal light variations with a period of 0.2539 days and mean amplitudes of 0.037 and 0.042 magnitudes in "i" and "V", respectively. We classified the spectrum of this star B2-3V with no evidence of variations in the profiles of its absorption lines in timescales of hours or days. The stellar spectrum is totally absent of emission lines. We detected unexpected faint radio continuum emission (between 0.4 and 0.8 mJy at 6-cm) showing a sinusoidal variation with a period of 12.8 days. The radio spectrum is thermal. We searched in the Very Large Array archive for radio continuum emission toward other 15 beta Cephei stars. None of these additional stars, some of them much closer to the Sun than V2187 Cyg, was detected, indicating that radio emission is extremely uncommon toward beta Cephei stars.

  19. Discovery of the magnetic field in the pulsating B star beta Cephei

    CERN Document Server

    Henrichs, H F; Verdugo, E; Schnerr, R S; Neiner, C; Donati, J -F; Catala, C; Shorlin, S L S; Wade, G A; Veen, P M; Nichols, J S; Damen, E M F; Talavera, A; Hill, G M; Kaper, L; Tijani, A M; Geers, V C; Wiersema, K; Plaggenborg, B; Rygl, K L J


    Although the star itself is not He enriched, the periodicity and the variability in the UV wind lines of the pulsating B1 IV star beta Cep are similar to what is observed in magnetic He-peculiar B stars, suggesting that beta Cep is magnetic. We searched for a magnetic field using spectropolarimetry. From UV spectroscopy, we analysed the wind variability and investigated the correlation with the magnetic data. Using 130 time-resolved circular polarisation spectra, obtained with the MuSiCoS spectropolarimeter at the 2m TBL from 1998 until 2005, we applied the least-squares deconvolution method on the Stokes V spectra and derived the longitudinal component of the integrated magnetic field over the visible hemisphere of the star. We performed a period analysis on the magnetic data and on EW measurements of UV wind lines obtained over 17 years. We also analysed the short- and long-term radial velocity variations, which are due to the pulsations and the 90-year binary motion. beta Cep hosts a sinusoidally varying m...

  20. Meiotic recombination in the beta globin gene cluster causing an error in prenatal diagnosis of beta thalassaemia.


    Camaschella, C.; Serra, A.; Saglio, G; Bertero, M T; Mazza, U; Terzoli, S; Brambati, B; Cremonesi, L.; Travi, M; Ferrari, M


    In the course of a prenatal diagnosis for beta thalassaemia by linkage analysis of restriction fragment length polymorphisms, a homozygous beta thalassaemia fetus was misdiagnosed as beta thalassaemia trait. Extensive studies of the polymorphic sites within the beta globin gene cluster in all the members of the family resulted in the conclusion that the paternal chromosome 11 of the newborn was different from that expected. Paternity was confirmed by HLA typing and blood group studies. The an...

  1. Heavy baryon spectroscopy from the lattice

    Energy Technology Data Exchange (ETDEWEB)

    Bowler, K.C.; Kenway, R.D.; Oliveira, O.; Richards, D.G.; Ueberholz, P. [Department of Physics and Astronomy, The University of Edinburgh, Edinburgh EH9 3JZ (Scotland); Lellouch, L.; Nieves, J.; Sachrajda, C.T.; Stella, N.; Wittig, H. [Physics Department, The University, Southampton SO17 1BJ (United Kingdom)


    The results of an exploratory lattice study of heavy baryon spectroscopy are presented. We have computed the full spectrum of the eight baryons containing a single heavy quark, on a 24{sup 3}{times}48 lattice at {beta}=6.2, using an {ital O}({ital a})-improved fermion action. We discuss the lattice baryon operators and give a method for isolating the contributions of the spin doublets ({Sigma},{Sigma}{sup {asterisk}}), ({Xi}{sup {prime}},{Xi}{sup {asterisk}}), and ({Omega},{Omega}{sup {asterisk}}) to the correlation function of the relevant operator. We compare our results with the available experimental data and find good agreement in both the charm and the {ital b}-quark sectors, despite the long extrapolation in the heavy quark mass needed in the latter case. We also predict the masses of several undiscovered baryons. We compute the {Lambda}-pseudoscalar meson and {Sigma}-{Lambda} mass splittings. Our results, which have errors in the range 10{endash}30{percent}, are in good agreement with the experimental numbers. For the {Sigma}{sup {asterisk}}-{Sigma} mass splitting, we find results considerably smaller than the experimental values for both the charm and the {ital b}-flavored baryons, although in the latter case the experimental results are still preliminary. This is also the case for the lattice results for the hyperfine splitting for the heavy mesons. {copyright} {ital 1996 The American Physical Society.}

  2. Nonlinear Dynamic Force Spectroscopy

    CERN Document Server

    Björnham, Oscar


    Dynamic force spectroscopy (DFS) is an experimental technique that is commonly used to assess information of the strength, energy landscape, and lifetime of noncovalent bio-molecular interactions. DFS traditionally requires an applied force that increases linearly with time so that the bio-complex under investigation is exposed to a constant loading rate. However, tethers or polymers can modulate the applied force in a nonlinear regime. For example, bacterial adhesion pili and polymers with worm-like chain properties are examples of structures that show nonlinear force responses. In these situations, the theory for traditional DFS cannot be readily applied. In this work we expand the theory for DFS to also include nonlinear external forces while still maintaining compatibility with the linear DFS theory. To validate the theory we modeled a bio-complex expressed on a stiff, an elastic and a worm-like chain polymer, using Monte Carlo methods, and assessed the corresponding rupture force spectra. It was found th...

  3. Quantitative velocity modulation spectroscopy (United States)

    Hodges, James N.; McCall, Benjamin J.


    Velocity Modulation Spectroscopy (VMS) is arguably the most important development in the 20th century for spectroscopic study of molecular ions. For decades, interpretation of VMS lineshapes has presented challenges due to the intrinsic covariance of fit parameters including velocity modulation amplitude, linewidth, and intensity. This limitation has stifled the growth of this technique into the quantitative realm. In this work, we show that subtle changes in the lineshape can be used to help address this complexity. This allows for determination of the linewidth, intensity relative to other transitions, velocity modulation amplitude, and electric field strength in the positive column of a glow discharge. Additionally, we explain the large homogeneous component of the linewidth that has been previously described. Using this component, the ion mobility can be determined.

  4. Analyzing Impedance Spectroscopy Results

    Institute of Scientific and Technical Information of China (English)

    Yoed Tsur; Sioma Baltianski


    In this contribution we briefly discuss several analysis techniques for impedance spectroscopy experiments. A number of different approaches, which differ even by the definition of the problem, are used in the literature. Some aimed towards finding an equivalent circuit. Others aimed towards finding directly dielectric properties of the material under an assumed model. Others towards finding distribution of relaxation times, either parametric or point-by point. No matter what the approach is, this will always be an ill-posed problem in the sense that there exist a large number of possible solutions that solve the problem (mathematically) equally well. Therefore some a-priori knowledge about the system must be used. In addition, we should remember that the ultimate goal is to get physical insight about the system.

  5. Theory overview on spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Ali, Ahmed


    A theoretical overview of the exotic spectroscopy in the charm and beauty quark sector is presented. These states are unexpected harvest from the e{sup +}e{sup -} and hadron colliders and a permanent abode for the majority of them has yet to be found. We argue that some of these states, in particular the Y{sub b}(10890) and the recently discovered states Z{sub b}(10610) and Z{sub b}(10650), discovered by the Belle collaboration are excellent candidates for tetraquark states [bq][ anti b anti q], with q=u,d light quarks. Theoretical analyses of the Belle data carried out in the tetraquark context is reviewed. (orig.)

  6. Advances in atomic spectroscopy

    CERN Document Server

    Sneddon, J


    This volume continues the series'' cutting-edge reviews on developments in this field. Since its invention in the 1920s, electrostatic precipitation has been extensively used in industrial hygiene to remove dust and particulate matter from gases before entering the atmosphere. This combination of electrostatic precipitation is reported upon in the first chapter. Following this, chapter two reviews recent advances in the area of chemical modification in electrothermal atomization. Chapter three consists of a review which deal with advances and uses of electrothermal atomization atomic absorption spectrometry. Flow injection atomic spectroscopy has developed rapidly in recent years and after a general introduction, various aspects of this technique are looked at in chapter four. Finally, in chapter five the use of various spectrometric techniques for the determination of mercury are described.

  7. Spectroscopy Made Easy: Evolution

    CERN Document Server

    Piskunov, Nikolai


    Context. The Spectroscopy Made Easy (SME) package has become a popular tool for analyzing stellar spectra, often in connection with large surveys or exoplanet research. SME has evolved significantly since it was first described in 1996, but many of the original caveats and potholes still haunt users. The main drivers for this paper are complexity of the modeling task, the large user community, and the massive effort that has gone into SME. Aims. We do not intend to give a comprehensive introduction to stellar atmospheres, but will describe changes to key components of SME: the equation of state, opacities, and radiative transfer. We will describe the analysis and fitting procedure and investigate various error sources that affect inferred parameters. Methods. We review the current status of SME, emphasizing new algorithms and methods. We describe some best practices for using the package, based on lessons learned over two decades of SME usage. We present a new way to assess uncertainties in derived stellar pa...

  8. Spectroscopy of radium

    Energy Technology Data Exchange (ETDEWEB)

    Mol, Aran; De, Subhadeep; Dammalapati, Umakanth; Jungmann, Klaus; Willmann, Lorenz [Kernfysisch Versneller Instituut, Rijksuniversiteit Groningen (Netherlands)


    Radium has been identified as a potential candidate for experiment al searches for violations of fundamental symmetries like parity (P), time reversal (T) and charge conjugation. In particular is shows a high sensitivity to T a nd P violating permanent electric dipole moments and also to atomic parity viola tion effects. This sensitivity arises from the unique atomic level scheme of rad ium. In the course of the setup of such experiments we need to improve the experimental data on radium. Within the TRI{mu}P (Trapped Radioactive Isotopes: microlaboratories for fundamental Physics) facility at KVI, we are setting up an radioactive atomic beam of {sup 225}Ra and the laser system for performing the spectroscopy. This is guided closely by the requirements for experimental searches for symmetry violating effect.

  9. Near-infrared spectroscopy

    Directory of Open Access Journals (Sweden)

    Virendra Jain


    Full Text Available Tissue ischaemia can be a significant contributor to increased morbidity and mortality. Conventional oxygenation monitoring modalities measure systemic oxygenation, but regional tissue oxygenation is not monitored. Near-infrared spectroscopy (NIRS is a non-invasive monitor for measuring regional oxygen saturation which provides real-time information. There has been increased interest in the clinical application of NIRS following numerous studies that show improved outcome in various clinical situations especially cardiac surgery. Its use has shown improved neurological outcome and decreased postoperative stay in cardiac surgery. Its usefulness has been investigated in various high risk surgeries such as carotid endarterectomy, thoracic surgeries, paediatric population and has shown promising results. There is however, limited data supporting its role in neurosurgical population. We strongly feel, it might play a key role in future. It has significant advantages over other neuromonitoring modalities, but more technological advances are needed before it can be used more widely into clinical practice.

  10. Transit spectroscopy with GTC

    Directory of Open Access Journals (Sweden)

    Osorio M.R. Zapatero


    Full Text Available Thanks to different ground-based surveys and space missions, nowadays we have a fairly large sample of discovered extra-solar planets to study and, without a doubt, this number will increase in the future. One of the most succesful techniques that allows us to prove the physical properties and atmospheric composition of these exoplanets is transmission spectroscopy. The level of precision that is require to measure these effects provides a technical challenge that is solved by using big telescopes and stable instruments to reach low noise levels. In this article, we will discuss the use of the 10m class telescope GTC to observed planetary transits in spectroscopic mode and some of the results that we are currently obtaining.

  11. Broadband local dielectric spectroscopy (United States)

    Labardi, M.; Lucchesi, M.; Prevosto, D.; Capaccioli, S.


    A route to extend the measurement bandwidth of local dielectric spectroscopy up to the MHz range has been devised. The method is based on a slow amplitude modulation at a frequency Ω of the excitation field oscillating at a frequency ω and the coherent detection of the modulated average electric force or force gradient at Ω. The cantilever mechanical response does not affect the measurement if Ω is well below its resonant frequency; therefore, limitations on the excitation field frequency are strongly reduced. Demonstration on a thin poly(vinyl acetate) film is provided, showing its structural relaxation spectrum on the local scale up to 45 °C higher than glass temperature, and nanoscale resolution dielectric relaxation imaging near conductive nanowires embedded in the polymer matrix was obtained up to 5 MHz frequency, with no physical reason to hinder further bandwidth extension.

  12. Wind-Forced Baroclinic Beta-Plumes (United States)

    Belmadani, A.; Maximenko, N. A.; Melnichenko, O.; Schneider, N.; Di Lorenzo, E.


    A planetary beta-plume is a classical example of oceanic circulation induced by a localized vorticity source or sink that allows an analytical description in simplistic cases. Its barotropic structure is a zonally-elongated, gyre-like cell governed by the Sverdrup circulation on the beta-plane. The dominant zonal currents, found west of the source/sink, are often referred to as zonal jets. This simple picture describes the depth-integrated flow. Previous studies have investigated beta-plumes in a reduced-gravity framework or using other simple models with a small number of vertical layers, thereby lacking representation of the vertical structure. In addition, most previous studies use a purely linear regime without considering the role of eddies. However, these jets are often associated with strong lateral shear that makes them unstable under increased forcing. The circulation in such a nonlinear regime may involve eddy-mean flow interactions, which modify the time-averaged circulation. Here, the baroclinic structures of linear and nonlinear wind-forced beta-plumes are studied using a continuously-stratified, primitive equation, eddy-permitting ocean model (ROMS). The model is configured in an idealized rectangular domain for the subtropical ocean with a flat bottom. The surface wind forcing is a steady anticyclonic Gaussian wind vortex, which provides a localized vorticity source in the center of the domain. The associated wind stress curl and Ekman pumping comprise downwelling in the vortex center surrounded by a ring of weaker upwelling. Under weak forcing, the simulated steady-state circulation corresponds well with a theoretical linear beta-plume. While its depth-integrated transport exhibits a set of zonal jets, consistent with Sverdrup theory, the baroclinic structure of the plume is remarkably complex. Relatively fast westward decay of the surface currents occurs simultaneously with the deepening of the lower boundary of the plume. This deepening suggests

  13. Broadband terahertz spectroscopy

    Institute of Scientific and Technical Information of China (English)

    Wenhui Fan


    1.Introduction Spanning the frequency range between the infrared (IR) radiation and microwaves,terahertz (THz) waves are,also known as T-rays,T-lux,or simply called THz,assigned to cover the electromagnetic spectrum typically from 100 GHz (1011 Hz) to 10 THz (1013 Hz),namely,from 3 mm to 30 μm in wavelength,although slightly different definitions have been quoted by different authors.For a very long time,THz region is an almost unexplored field due to its rather unique location in the electromagnetic spectrum.Well-known techniques in optical or microwave region can not be directly employed in the THz range because optical wavelengths are too short and microwave wavelengths are too long compared to THz wavelengths.%An overview of the major techniques to generate and detect THz radiation so far, especially the major approaches to generate and detect coherent ultra-short THz pulses using ultra-short pulsed laser, has been presented. And also, this paper, in particularly, focuses on broadband THz spectroscopy and addresses on a number of issues relevant to generation and detection of broadband pulsed THz radiation as well as broadband time-domain THz spectroscopy (THz-TDS) with the help of ultra-short pulsed laser. The time-domain waveforms of coherent ultra-short THz pulses from photoconductive antenna excited by femtosecond laser with different pulse durations and their corresponding Fourier-transformed spectra have been obtained via the numerical simulation of ultrafast dynamics between femtosecond laser pulse and photoconductive material. The origins of fringes modulated on the top of broadband amplitude spectrum, which is measured by electric-optic detector based on thin nonlinear crystal and extracted by fast Fourier transformation, have been analyzed and the major solutions to get rid of these fringes are discussed.

  14. Distinct down-regulation of cardiac beta 1- and beta 2-adrenoceptors in different human heart diseases. (United States)

    Steinfath, M; Geertz, B; Schmitz, W; Scholz, H; Haverich, A; Breil, I; Hanrath, P; Reupcke, C; Sigmund, M; Lo, H B


    Cardiac beta-adrenoceptor density and beta 1- and beta 2-subtype distribution were examined in human left ventricular myocardium from transplant donors serving as controls and from patients with mitral valve stenosis, aortic valve stenosis, idiopathic dilated cardiomyopathy, and ischaemic cardiomyopathy respectively. The total beta-adrenoceptor density was similar in transplant donors and patients with moderate heart failure (NYHA II-III) due to mitral valve stenosis, but was markedly reduced in all forms of severe heart failure (NYHA III-IV) studied. A reduction of both beta 1- and beta 2-adrenoceptors was found in patients with severe heart failure due to mitral valve stenosis or ischaemic cardiomyopathy. In contrast, a selective down-regulation of beta 1-adrenoceptors with unchanged beta 2-adrenoceptors and hence a relative increase in the latter was observed in idiopathic dilated cardiomyopathy and aortic valve stenosis. It is concluded that the extent of total beta-adrenoceptor down-regulation is related to the degree of heart failure. Selective loss of beta 1-adrenoceptors is not specific for idiopathic dilated cardiomyopathy but also occurs in aortic valve stenosis. Changes in beta 1- and beta 2-subtype distribution are rather related to the aetiology than to the clinical degree of heart failure.

  15. Rheumatoid factors from patients with rheumatoid arthritis react with Des-Lys58-beta 2m, modified beta 2-microglobulin

    DEFF Research Database (Denmark)

    Williams, R C; Nissen, Mogens Holst; Malone, C C


    Ten polyclonal IgM rheumatoid factor (RF) preparations, affinity-purified from IgG columns, from patients with rheumatoid arthritis were studied for their ELISA reactivity with native beta 2m in parallel with Lys58-cleaved beta 2m and Des-Lys58-beta 2m, the latter representing cleavage products...

  16. Beta-lactamases of Mycobacterium tuberculosis and Mycobacterium kansasii. (United States)

    Segura, C; Salvadó, M


    Re-emergence of infectious diseases caused by mycobacteria as well as the emergence of multiresistant strains of Mycobacterium has promoted the research on the use of beta-lactames in the treatment of such diseases. Mycobacteria produce beta-lactamases: M. tuberculosis produces a wide-spectrum beta-lactamase whose behaviour mimicks those of Gram-negative bacteria. M. kansasii produces also beta-lactamase which can be inhibited by clavulanic acid. An overview on beta-lactamases from both species is reported.

  17. Anxiolytics not acting at the benzodiazepine receptor: beta blockers. (United States)

    Tyrer, P


    1. Although there is clear evidence for many controlled trials in the past 25 years that beta blockers are effective in anxiety disorders clear indications for their use are lacking. 2. The balance of evidence suggests that the mechanism of action of beta-blocking drugs is through peripheral blockade of beta-mediated symptoms. 3. Most evidence to the efficacy of beta-blockers comes from study of their use in generalized anxiety and in acute stress. 4. Because beta-blockers carry no risks of pharmacological dependence they may be preferred to many other anti-anxiety drugs.

  18. Probing the N = Z Line via {beta} Decay

    Energy Technology Data Exchange (ETDEWEB)

    Markku Oinonen


    This contribution reports several beta-decay studies performed at ISOLDE On-Line Mass Separator at CERN recently for nuclei close to N = Z line. Beta decay of {sup 58}Zn provides a possibility to compare Gamow-Teller strength extracted from complementary beta-decay studies and charge-exchange reactions. Measurement on beta-decay half-life of {sup 70}Kr shows importance of experimental information in modeling the path of the astrophysical rp process. Decay of {sup 71}Kr is an example of a mirror beta decay and extends the systematics of these particular decays towards highly deformed region close to A = 80.

  19. Infrared heterodyne spectroscopy in astronomy (United States)

    Betz, A.


    A heterodyne spectrometer was constructed and applied to problems in infrared astronomical spectroscopy. The instrument offers distinct observational advantages for the detection and analysis of individual spectral lines at Doppler-limited resolution. Observations of carbon dioxide in planetary atmospheres and ammonia in circumstellar environments demonstrate the substantial role that infrared heterodyne techniques will play in the astronomical spectroscopy of the future.

  20. Spectroscopy, Understanding the Atom Series. (United States)

    Hellman, Hal

    This booklet is one of the "Understanding the Atom" Series. The science of spectroscopy is presented by a number of topics dealing with (1) the uses of spectroscopy, (2) its origin and background, (3) the basic optical systems of spectroscopes, spectrometers, and spectrophotometers, (4) the characteristics of wave motion, (5) the…

  1. The light meson spectroscopy program

    Directory of Open Access Journals (Sweden)

    Smith Elton S.


    Full Text Available Recent discoveries of a number of unexpected new charmomium-like meson states at the BaBar and Belle B-factories have demonstrated how little is still known about meson spectroscopy. In this talk we will review recent highlights of the light quark spectroscopy from collider and fixed target experiments.

  2. Diffusion measurements by Raman spectroscopy

    DEFF Research Database (Denmark)

    Hansen, Susanne Brunsgaard; Shapiro, Alexander; Berg, Rolf W.;

    Poster "Diffusion measurements by Raman spectroscopy", See poster at "Diffusion measurements by Raman spectroscopy", See poster at

  3. The light meson spectroscopy program

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Elton S. [JLAB


    Recent discoveries of a number of unexpected new charmomium-like meson states at the BaBar and Belle B-factories have demonstrated how little is still known about meson spectroscopy. In this talk we will review recent highlights of the light quark spectroscopy from collider and fixed target experiments.

  4. Factors affecting drug adsorption on beta zeolites. (United States)

    Pasti, Luisa; Sarti, Elena; Cavazzini, Alberto; Marchetti, Nicola; Dondi, Francesco; Martucci, Annalisa


    The adsorption behaviour of three commonly used drugs, namely ketoprofen, hydrochlorothiazide and atenolol, from diluted aqueous solutions on beta zeolites with different SiO2/Al2O3 ratio (i.e. 25, 38 and 360) was investigated by changing the ionic strength and the pH, before and after thermal treatment of the adsorbents. The selective adsorption of drugs was confirmed by thermogravimetry and X-ray diffraction. The adsorption capacity of beta zeolites was strongly dependent on both the solution pH and the alumina content of the adsorbent. Such a remarkable difference was interpreted as a function of the interactions between drug molecules and zeolite surface functional groups. Atenolol was readily adsorbed on the less hydrophobic zeolite, under pH conditions in which electrostatic interactions were predominant. On the other hand, ketoprofen adsorption was mainly driven by hydrophobic interactions. For undissociated molecules the adsorption capability increased with the increase of hydrophobicity.

  5. Beta-haemolytic streptococci in acute pharyngitis. (United States)

    Boukadida, J; Hannechi, N; Boukadida, N; Ben Said, H; Elmherbech, H; Errai, S


    To determine the role and importance of beta-haemolytic streptococci in acute pharyngitis and its relative susceptibility to antibiotics, we cultured samples from 143 patients (age range: 3-72 years) who presented over a 5-month period in 2001 at three primary health care centres in Sousse, Tunisia. The cultures yielded 80 beta-haemolytic streptococci (59 group A streptococci and 21 non-group A streptococci). All strains were susceptible to benzylpenicillin, amoxicillin, chloramphenicol, rifampicin and pristinamycin. Susceptibility was variable in erythromycin, tetracycline, fosfomycin, telithromycin and levofloxacin. Minimum inhibitory concentrations were determined by E-test for penicillin, erythromycin and levofloxacin. Our results confirm that penicillin is still the reference treatment for acute pharyngitis. However, to minimize the potential for complications arising from its use, continued vigilance is required.

  6. Source preparations for alpha and beta measurements

    Energy Technology Data Exchange (ETDEWEB)

    Holm, E. [Risoe National Lab., Roskilde (Denmark)


    Regarding alpha particle emitters subject for environmental studies, electrodeposition or co-precipitation as fluorides are the most common methods. For electro deposition stainless steel is generally used as cathode material but also other metals such as Ni, Ag, and Cu showed promising results. The use of other anode material than platinum, such as graphite should be investigated. For other purposes such as optimal resolution other more sophisticated methods are used but often resulting in poorer recovery. For beta particle emitters the type of detection system will decide the source preparation. Similar methods as for alpha particle emitters, electrodeposition or precipitation techniques can be used. Due to the continuous energy distribution of the beta pulse height distribution a high resolution is not required. Thicker sources from the precipitates or a stable isotopic carrier can be accepted but correction for absorption in the source must be done. (au)

  7. Decay Spectroscopy of Neutron-Rich Cd Around the N = 82 Shell Closure (United States)

    Bernier, Nikita; Dillmann, Iris; Kruecken, Reiner; Griffin Collaboration


    The neutron-rich region around A = 132 is of special interest for nuclear astrophysics and nuclear structure. This region is connected with the second r-process abundance peak at A 130 and the waiting-point nuclei around N = 82. For nuclear structure studies, the neighbours of the doubly-magic 132Sn (Z = 50, N = 82) are an ideal test ground for shell model predictions. The beta-decay of the N = 82 isotope 130Cd into 130In was first investigated a decade ago, but the information for states of the lighter indium isotopes (128,129In) is still limited. In the present experiment, a detailed gamma-spectroscopy of the beta-decay of 128-132Cd was achieved with the newly commissioned GRIFFIN (Gamma-Ray Infrastructure For Fundamental Investigations of Nuclei) gamma-ray spectrometer, which is capable of measuring down to rates of 0.1 pps. The low-energy cadmium isotopes were implanted into a movable tape at the central focus of the array from the ISAC-I facility at TRIUMF. The beta-tagging was performed using the auxiliary beta-particle detector SCEPTAR. The required beta-gamma(-gamma) coincidence data in high statistics needed to fill the spectroscopic gaps described in literature were obtained. The ongoing analysis of these data will be presented. Work supported by the Natural Sciences and Engineering Research Council of Canada and the National Research Council of Canada.

  8. Autoradiographic localization of beta-adrenoreceptors in rat uterus

    Energy Technology Data Exchange (ETDEWEB)

    Tolszczuk, M.; Pelletier, G.


    The inhibitory effects of catecholamines on uterine smooth muscle are known to be mediated through beta-adrenergic receptors. To investigate further the distribution of these receptors in the rat uterus, we utilized in vitro autoradiography using ( SVI)-cyanopindolol (CYP), a specific beta-receptor ligand that has equal activity for both beta 1- and beta 2-receptor subtypes. The specificity of the labeling and the characterization of receptor subtypes in different cell types were achieved by displacement of radioligand with increasing concentrations of zinterol, a beta-adrenergic agonist with preferential affinity for the beta 2-adrenoreceptor subtype, and practolol, a beta-adrenergic antagonist that binds preferentially to the beta 1-subtype. Quantitative estimation of ligand binding was performed by densitometry. It was shown that the vast majority of beta-adrenoreceptors were of the beta 2-subtype and were found in high concentration not only in the myometrium but also in the endometrial and serosal epithelia. Specific labeling was also observed in glandular elements. These results suggest that beta-adrenoreceptors might be involved in different functions in the uterus.

  9. Beta Testing of Persistent Passive Acoustic Monitors (United States)


    solution. This translates into longer deployments on platforms such as gliders with limited hotel load. 2. the DMON is specifically designed for low...filter or filter bank may improve performance • Glider and Profiler motors and pumps are noisy , however duty cycle of noise is low. Therefore they are...power making it ideal for low hotel load autonomous vehicles like gliders. The format shown here was provided to beta-test clients working on gliders and

  10. SPL and Beta Beams to the Frejus

    CERN Document Server

    Mezzetto, M


    Physics potential of a conventional neutrino beam generated by the 2.2 GeV, 4MW, Superconducting Proton Linac and of a Beta Beam fired to a gigantic water Ccaronerenkov detector hosted below Frejus, 130 km away from CERN, are briefly summarized. theta/sub 13/ sensitivity could be improved by up to 3 orders of magnitude with respect to the present experimental limit and a first sensitive search for leptonic CP violation could be performed.

  11. Beta-decay of Cu-56

    NARCIS (Netherlands)

    Ramdhane, M; Baumann, P; Knipper, A; Walter, G; Janas, Z; Plochocki, A; Aysto, J; Dendooven, P; Jokinen, A; Oinonen, M; Pentila, H; Liu, W; Gorska, M; Grawe, H; Hu, Z; Kirchner, R; Klepper, O; Roeckl, E; Fujita, Y; Brown, BA


    By measuring positrons and P-delayed gamma-rays emitted from mass-separated sources, the decay of Cu-56 (4(+),T-z = -1,T = 1) to states in the doubly-magic nucleus Ni-56 was Studied for the first time. The half-life of Cu-56 was measured to be 78(15) ms, and four beta-delayed gamma-rays were assigne

  12. A background free double beta decay experiment


    Giomataris, Ioannis


    We present a new detection scheme for rejecting backgrounds in neutrino less double beta decay experiments. It relies on the detection of Cherenkov light emitted by electrons in the MeV region. The momentum threshold is tuned to reach a good discrimination between background and good events. We consider many detector concepts and a range of target materials. The most promising is a high-pressure 136Xe emitter for which the required energy threshold is easily adjusted. Combination of this conc...

  13. Alpha-beta bidirectional associative memories


    María Elena Acevedo Mosqueda; Cornelio Yáñez Márquez


    Most models of Bidirectional associative memories intend to achieve that all trained pattern correspond to stable states; however, this has not been possible. Also, none of the former models has been able to recall all the trained patterns. In this work we introduce a new model of bidirectional associative memory which is not iterative and has no stability problems. It is based on the Alpha-Beta associative memories. This model allows perfect recall of all trained patterns, with no ambiguity ...

  14. Precision mass measurements utilizing beta endpoints

    CERN Document Server

    Moltz, D M; Kern, B D; Noma, H; Ritchie, B G; Toth, K S


    A technique for precise determination of beta endpoints with an intrinsic germanium detector has been developed. The energy calibration is derived from gamma -ray photopeak measurements. This analysis procedure has been checked with a /sup 27/Si source produced in a (p, n) reaction on a /sup 27/Al target and subsequently applied to mass separated samples of /sup 76/Rb, /sup 77/Rb and /sup 78/Rb. Results indicate errors <50 keV are obtainable. (29 refs).

  15. High-{beta} disruption in tokamaks

    Energy Technology Data Exchange (ETDEWEB)

    Park, W.; Fredrickson, E.D.; Janos, A. [and others


    Three dimensional MHD simulations of high-{beta} plasmas show that toroidally localized high-n ballooning modes can be driven unstable by the local pressure steepening which arises from the evolution of low-n modes. Nonlinearly, the high-n mode becomes even more localized and produces a strong local pressure bulge which destroys the flux surfaces resulting in a thermal quench. The flux surfaces then recover temporarily but now contain large magnetic islands. This scenario is supported by experimental data.

  16. Raman Spectroscopy for Clinical Oncology

    Directory of Open Access Journals (Sweden)

    Michael B. Fenn


    Full Text Available Cancer is one of the leading causes of death throughout the world. Advancements in early and improved diagnosis could help prevent a significant number of these deaths. Raman spectroscopy is a vibrational spectroscopic technique which has received considerable attention recently with regards to applications in clinical oncology. Raman spectroscopy has the potential not only to improve diagnosis of cancer but also to advance the treatment of cancer. A number of studies have investigated Raman spectroscopy for its potential to improve diagnosis and treatment of a wide variety of cancers. In this paper the most recent advances in dispersive Raman spectroscopy, which have demonstrated promising leads to real world application for clinical oncology are reviewed. The application of Raman spectroscopy to breast, brain, skin, cervical, gastrointestinal, oral, and lung cancers is reviewed as well as a special focus on the data analysis techniques, which have been employed in the studies.

  17. Inhibition of human and rat 11beta-hydroxysteroid dehydrogenase type 1 by 18beta-glycyrrhetinic acid derivatives. (United States)

    Su, Xiangdong; Vicker, Nigel; Lawrence, Harshani; Smith, Andrew; Purohit, Atul; Reed, Michael J; Potter, Barry V L


    11beta-Hydroxysteroid dehydrogenase type 1 (11beta-HSD1) plays an important role in regulating the cortisol availability to bind to corticosteroid receptors within specific tissue. Recent advances in understanding the molecular mechanisms of metabolic syndrome indicate that elevation of cortisol levels within specific tissues through the action of 11beta-HSD1 could contribute to the pathogenesis of this disease. Therefore, selective inhibitors of 11beta-HSD1 have been investigated as potential treatments for metabolic diseases, such as diabetes mellitus type 2 or obesity. Here we report the discovery and synthesis of some 18beta-glycyrrhetinic acid (18beta-GA) derivatives (2-5) and their inhibitory activities against rat hepatic11beta-HSD1 and rat renal 11beta-HSD2. Once the selectivity over the rat type 2 enzyme was established, these compounds' ability to inhibit human 11beta-HSD1 was also evaluated using both radioimmunoassay (RIA) and homogeneous time resolved fluorescence (HTRF) methods. The 11-modified 18beta-GA derivatives 2 and 3 with apparent selectivity for rat 11beta-HSD1 showed a high percentage inhibition for human microsomal 11beta-HSD1 at 10 microM and exhibited IC50 values of 400 and 1100 nM, respectively. The side chain modified 18beta-GA derivatives 4 and 5, although showing selectivity for rat 11beta-HSD1 inhibited human microsomal 11beta-HSD1 with IC50 values in the low micromolar range.

  18. Non-invasive in vivo determination of the carotenoids beta-carotene and lycopene concentrations in the human skin using the Raman spectroscopic method

    Energy Technology Data Exchange (ETDEWEB)

    Darvin, M E [Center of Experimental and Applied Cutaneous Physiology (CCP), Department of Dermatology, Charite University Hospital, Berlin (Germany); Gersonde, I [Institute of Medical Physics and Laser Medicine, Charite University Hospital, Berlin (Germany); Meinke, M [Institute of Medical Physics and Laser Medicine, Charite University Hospital, Berlin (Germany); Sterry, W [Center of Experimental and Applied Cutaneous Physiology (CCP), Department of Dermatology, Charite University Hospital, Berlin (Germany); Lademann, J [Center of Experimental and Applied Cutaneous Physiology (CCP), Department of Dermatology, Charite University Hospital, Berlin (Germany)


    Resonance Raman spectroscopy was used as a fast and non-invasive optical method of measuring the absolute concentrations of beta-carotene and lycopene in living human skin. Beta-carotene and lycopene have different absorption values at 488 and 514.5 nm and, consequently, the Raman lines for beta-carotene and lycopene have different scattering efficiencies at 488 and 514.5 nm excitations. These differences were used for the determination of the concentrations of beta-carotene and lycopene. Using multiline Ar{sup +} laser excitation, clearly distinguishable carotenoid Raman spectra can be obtained which are superimposed on a large fluorescence background. The Raman signals are characterized by two prominent Stokes lines at 1160 and 1525 cm{sup -1}, which have nearly identical relative intensities. Both substances were detected simultaneously. The Raman spectra are obtained rapidly, i.e. within about 10 s, and the required laser light exposure level is well within safety standards. The disturbance of the measurements by non-homogeneous skin pigmentation was avoided by using a relatively large measuring area of 35 mm{sup 2}. It was shown that beta-carotene and lycopene distribution in human skin strongly depends upon the skin region studied and drastically changed inter-individually. Skin beta-carotene and lycopene concentrations are lower in smokers than in non-smokers and higher in the vegetarian group.

  19. Non-invasive in vivo determination of the carotenoids beta-carotene and lycopene concentrations in the human skin using the Raman spectroscopic method (United States)

    Darvin, M. E.; Gersonde, I.; Meinke, M.; Sterry, W.; Lademann, J.


    Resonance Raman spectroscopy was used as a fast and non-invasive optical method of measuring the absolute concentrations of beta-carotene and lycopene in living human skin. Beta-carotene and lycopene have different absorption values at 488 and 514.5 nm and, consequently, the Raman lines for beta-carotene and lycopene have different scattering efficiencies at 488 and 514.5 nm excitations. These differences were used for the determination of the concentrations of beta-carotene and lycopene. Using multiline Ar+ laser excitation, clearly distinguishable carotenoid Raman spectra can be obtained which are superimposed on a large fluorescence background. The Raman signals are characterized by two prominent Stokes lines at 1160 and 1525 cm-1, which have nearly identical relative intensities. Both substances were detected simultaneously. The Raman spectra are obtained rapidly, i.e. within about 10 s, and the required laser light exposure level is well within safety standards. The disturbance of the measurements by non-homogeneous skin pigmentation was avoided by using a relatively large measuring area of 35 mm2. It was shown that beta-carotene and lycopene distribution in human skin strongly depends upon the skin region studied and drastically changed inter-individually. Skin beta-carotene and lycopene concentrations are lower in smokers than in non-smokers and higher in the vegetarian group.

  20. Serum beta2-microglobulin in cadmium exposed workers. (United States)

    Piscator, M


    In cadmium exposed workers with renal tubular dysfunction the determination of beta2m in urine is an important diagnostic test. Cadmium exposure's influence on serum beta2m levels and its relationship to urinary excretion of beta2m were studied in 24 cadmium exposed workers with normal serum creatinine levels (less than 10 mg/l)) and no obvious tubular dysfunction. With increasing blood levels of cadmium beta2m was found to increase in serum. There was no concomitant increase in the urinary excretion of beta2m. Serum beta2m was not dependent on serum creatinine within the range studied. The results suggest that for evaluating renal glomerular function in cadmium exposed workers, it might be better to use the serum creatinine level, creatinine clearance or inulin clearance since beta2m might give some false positive results.

  1. Beta Beams for Precision Measurements of Neutrino Oscillation Parameters

    CERN Document Server

    Wildner, E; Hansen, C; De Melo Mendonca, T; Stora, T; Damjanovic, S; Payet, J; Chancé, A; Zorin, V; Izotov, I; Rasin, S; Sidorov, A; Skalyga, V; De Angelis, G; Prete, G; Cinausero, M; Kravchuk, V; Gramegna, F; Marchi, T; Collazuol, G; Mezzetto, M; Delbar, T; Loiselet, M; Keutgen, T; Mitrofanov, S; Burt, G; Dexter, A; Lamy, T; Latrasse, L; Marie-Jeanne, M; Sortais, P; Thuillier, T; Debray, F; Trophime, C; Hass, M; Hirsh, T; Berkovits, D; Stahl, A; Vardaci, E; Di Nitto, A; Brondi, A; La Rana, G; Moro, R; De Rosa, G; Palladino, V


    Neutrino oscillations have implications for the Standard Model of particle physics. The CERN Beta Beam has outstanding capabilities to contribute to precision measurements of the parameters governing neutrino oscillations. The FP7 collaboration EUROnu (2008-2012) is a design study that will review three facilities (Super-Beams, Beta Beams and Neutrino Factories) and perform a cost assessment that, coupled with the physics performance, will give means to the European research authorities to make decisions on future European neutrino oscillation facilities. ”Beta Beams” produce collimated pure electron (anti)neutrinos by accelerating beta active ions to high energies and having them decay in a storage ring. Using existing machines and infrastructure is an advantage for the cost evaluation; however, this choice is also constraining the Beta Beams. Recent work to make the Beta Beam facility a solid option will be described: production of Beta Beam isotopes, the 60 GHz pulsed ECR source development, integratio...

  2. Reinvestigation of the beta-decay of {sup 110}Mo

    Energy Technology Data Exchange (ETDEWEB)

    Wang, J.C.; Dendooven, P.; Hankonen, S.; Huikari, J.; Jokinen, A.; Kolhinen, V.S.; Lhersonneau, G.; Nieminen, A.; Peraejaervi, K.; Rinta-Antila, S.; Aeystoe, J. [Department of Physics, University of Jyvaeskylae, FIN-40351, Jyvaeskylae (Finland)


    The beta-decay of the neutron-rich nucleus {sup 110}Mo, separated by the IGISOL on-line mass separator from other fission products, has been investigated by using beta-gamma and gamma-gamma coincidence techniques. The decay scheme of {sup 110}Mo has been revised, including 3 new excited states and 7 new {gamma} transitions in {sup 110}Tc. The {beta}-feedings were measured and log ft values and B(GT) values were deduced based on a Q{sub {beta}}-value from systematics. Three excited 1{sup +} states in {sup 110}Tc fed by spin-flip allowed-unhindered beta transitions were identified. The deduced beta-decay strengths are compared with the Gamow-Teller strength distribution obtained from a macroscopic-microscopic calculation. The role of the asymptotic quantum numbers in the context of the allowed beta-decay is discussed. (orig.)

  3. Genetic analysis of beta1 integrin "activation motifs" in mice

    DEFF Research Database (Denmark)

    Czuchra, Aleksandra; Meyer, Hannelore; Legate, Kyle R


    tails, leading to tail separation and integrin activation. We analyzed mice in which we mutated the tyrosines of the beta1 tail and the membrane-proximal aspartic acid required for the salt bridge. Tyrosine-to-alanine substitutions abolished beta1 integrin functions and led to a beta1 integrin......-null phenotype in vivo. Surprisingly, neither the substitution of the tyrosines with phenylalanine nor the aspartic acid with alanine resulted in an obvious defect. These data suggest that the NPXY motifs of the beta1 integrin tail are essential for beta1 integrin function, whereas tyrosine phosphorylation......Akey feature of integrins is their ability to regulate the affinity for ligands, a process termed integrin activation. The final step in integrin activation is talin binding to the NPXY motif of the integrin beta cytoplasmic domains. Talin binding disrupts the salt bridge between the alpha/beta...

  4. Enhanced synergism of antibiotics with zinc oxide nanoparticles against extended spectrum {beta}-lactamase producers implicated in urinary tract infections

    Energy Technology Data Exchange (ETDEWEB)

    Bhande, Rashmi M., E-mail:; Khobragade, C. N., E-mail: [Swami Ramanand Teerth Marathwada University, School of Life Sciences (India); Mane, R. S., E-mail:; Bhande, S., E-mail: [Swami Ramanand Teerth Marathwada University, School of Physical Sciences (India)


    In this study, enhanced synergistic bioactivity of zinc oxide nanoparticles (ZnO NPs) with {beta}-lactam antibiotics were evaluated against a panel of clinically isolated extended spectrum {beta}-lactamase producers implicated in urinary tract infections. Chemically synthesized zinc oxide nanoparticles (15 nm) were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), high resolution transmittance electron microscopy (HR-TEM), selective area electron diffraction (SAED), X-ray photoelectron spectroscopy (XPS), and UV-Visible spectrophotometry techniques. The antimicrobial potency (10 {+-} 0.66, 12, 11.33 {+-} 1.10, and 0.7 {+-} 0.66 mm inhibiting zone) and minimum inhibitory concentrations (80, 60, 30, 50 {mu}g/ml) of ZnO NPs were tested separately whereas time-kill and membrane leakage assays were evaluated in combination with ZnO NPs+ cefotaxime, ampicillin, ceftriaxone, cefepime against the {beta}-lactamase producer strains of E. coli, K. pneumoniae, S. paucimobilis, and P. aeruginosa, respectively. Time-kill curve dynamics of ZnO NPs with {beta}-lactam antibiotics revealed enhanced bactericidal activity (50, 85, 58, 50 % fold inhibition) by delaying the exponential and stationary phases of all isolates when tested separately. Posttime-kill effect was studied on cell membrane by assaying leakage of reducing sugars (130.2, 124.7, 137, and 115.8 {mu}g/bacterial dry weight of 1 mg ({mu}g/mg) and proteins (15, 10, 16, 18 {mu}g/mg). These assays revealed that membrane leakage was due to synergism of ZnO NPs+ {beta}-lactam antibiotics which successfully damage cell membrane thereby leading to death of all ESBL producers. The results demonstrate the utilization of ZnO NPs as a potentiator of {beta}-lactam antibiotics and suggest the possibility to use nanoparticles in a combination therapy to treat UTI.

  5. Alpha-helical, but not beta-sheet, propensity of proline is determined by peptide environment. (United States)

    Li, S C; Goto, N K; Williams, K A; Deber, C M


    Proline is established as a potent breaker of both alpha-helical and beta-sheet structures in soluble (globular) proteins. Thus, the frequent occurrence of the Pro residue in the putative transmembrane helices of integral membrane proteins, particularly transport proteins, presents a structural dilemma. We propose that this phenomenon results from the fact that the structural propensity of a given amino acid may be altered to conform to changes imposed by molecular environment. To test this hypothesis on proline, we synthesized model peptides of generic sequence H2N-(Ser-LyS)2-Ala- Leu-Z-Ala-Leu-Z-Trp-Ala-Leu-Z-(Lys-Ser)3-OH (Z = Ala and/or Pro). Peptide conformations were analyzed by circular dichroism spectroscopy in aqueous buffer, SDS, lysophosphatidylglycerol micelles, and organic solvents (methanol, trifluoroethanol, and 2-propanol). The helical propensity of Pro was found to be greatly enhanced in the membrane-mimetic environments of both lipid micelles and organic solvents. Proline was found to stabilize the alpha-helical conformation relative to Ala at elevated temperatures in 2-propanol, an observation that argues against the doctrine that Pro is the most potent alpha-helix breaker as established in aqueous media. Parallel studies in deoxycholate micelles of the temperature-induced conformational transitions of the single-spanning membrane bacteriophage IKe major coat protein, in which the Pro-containing wild type was compared with Pro30 --> Ala mutant, Pro was found to protect the helix, but disrupt the beta-sheet structure as effectively as it does to model peptides in water. The intrinsic capacity of Pro to disrupt beta-sheets was further reflected in a survey of porins where Pro was found to be selectively excluded from the core of membrane-spanning beta-sheet barrels. The overall data provide a rationale for predicting and understanding the structural consequences when Pro occurs in the context of a membrane.

  6. Operando fuel cell spectroscopy (United States)

    Kendrick, Ian Michael

    The active state of a catalyst only exists during catalysis (1) provided the motivation for developing operando spectroscopic techniques. A polymer electrolyte membrane fuel cell (PEMFC) was designed to interface with commercially available instruments for acquisition of infrared spectra of the catalytic surface of the membrane electrode assembly (MEA) during normal operation. This technique has provided insight of the complex processes occurring at the electrode surface. Nafion, the solid electrolyte used in most modern-day polymer electrolyte membrane fuel cells (PEMFC), serves many purposes in fuel cell operation. However, there is little known of the interface between Nafion and the electrode surface. Previous studies of complex Stark tuning curves of carbon monoxide on the surface of a platinum electrode were attributed the co-adsorption of bisulfite ions originating from the 0.5M H2SO4 electrolyte used in the study(2). Similar tuning curves obtained on a fuel cell MEA despite the absence of supplemental electrolytes suggest the adsorption of Nafion onto platinum (3). The correlation of spectra obtained using attenuated total reflectance spectroscopy (ATR) and polarization modulated IR reflection-absorption spectroscopy (PM-IRRAS) to a theoretical spectrum generated using density functional theory (DFT) lead to development of a model of Nafion and platinum interaction which identified participation of the SO3- and CF3 groups in Nafion adsorption. The use of ethanol as a fuel stream in proton exchange membrane fuel cells provides a promising alternative to methanol. Relative to methanol, ethanol has a greater energy density, lower toxicity and can be made from the fermentation of biomass(4). Operando IR spectroscopy was used to study the oxidation pathway of ethanol and Stark tuning behavior of carbon monoxide on Pt, Ru, and PtRu electrodes. Potential dependent products such as acetaldehyde, acetic acid and carbon monoxide are identified as well as previously

  7. Spectroscopy from Space (United States)

    Clark, R. N.; Swayze, G. A.; Carlson, R.; Grundy, W.; Noll, K.


    This chapter reviews detection of materials on solid and liquid (lakes and ocean) surfaces in the solar system using ultraviolet to infrared spectroscopy from space, or near space (high altitude aircraft on the Earth), or in the case of remote objects, earth-based and earth-orbiting telescopes. Point spectrometers and imaging spectrometers have been probing the surfaces of our solar system for decades. Spacecraft carrying imaging spectrometers are currently in orbit around Mercury, Venus, Earth, Mars, and Saturn, and systems have recently visited Jupiter, comets, asteroids, and one spectrometer-carrying spacecraft is on its way to Pluto. Together these systems are providing a wealth of data that will enable a better understanding of the composition of condensed matter bodies in the solar system. Minerals, ices, liquids, and other materials have been detected and mapped on the Earth and all planets and/or their satellites where the surface can be observed from space, with the exception of Venus whose thick atmosphere limits surface observation. Basaltic minerals (e.g., pyroxene and olivine) have been detected with spectroscopy on the Earth, Moon, Mars and some asteroids. The greatest mineralogic diversity seen from space is observed on the Earth and Mars. The Earth, with oceans, active tectonic and hydrologic cycles, and biological processes, displays the greatest material diversity including the detection of amorphous and crystalline inorganic materials, organic compounds, water and water ice. Water ice is a very common mineral throughout the Solar System and has been unambiguously detected or inferred in every planet and/or their moon(s) where good spectroscopic data has been obtained. In addition to water ice, other molecular solids have been observed in the solar system using spectroscopic methods. Solid carbon dioxide is found on all systems beyond the Earth except Pluto, although CO2 sometimes appears to be trapped in other solids rather than as an ice on some

  8. Effects of calcium impurity on phase relationship, ionic conductivity and microstructure of Na$^{+}$-$\\beta/beta"$-alumina solid electrolyte

    Indian Academy of Sciences (India)



    Ca-doped Na$^{+}$-$\\beta/beta"$-alumina was synthesized using a solid-state reaction. The changes in the properties of Na$^{+}$-$\\beta/beta"$-alumina resulting from the presence of Ca impurity were studied. Ca (0–5 wt%) was added to the respective samples, which were then sintered. The specimens were characterized using X-ray diffraction, scanningelectron microscopy, densimetry and impedance analysis. In the sintered specimens, the $\\beta"$-alumina phase fraction decreased as Ca content increased, whereas the relative sintered density increased. The surface morphology of Cadoped Na$^{+}$-$\\beta/beta"$-alumina specimens showed a Ca-rich layer, which was the main cause of increase in the specificresistance.

  9. Meson spectroscopy with COMPASS

    CERN Document Server

    Nerling, Frank


    The COMPASS fixed-target experiment at CERN SPS is dedicated to the study of hadron structure and dynamics. In the physics programme using hadron beams, the focus is on the detection of new states, in particular the search for $J^{PC}$ exotic states and glueballs. After a short pilot run in 2004 (190 GeV/c negative pion beam, lead target), we started our hadron spectroscopy programme in 2008 by collecting an unprecedented statistics with a negative hadron beam (190 GeV/c) on a liquid hydrogen target. A similar amount of data with positive hadron beam (190 GeV/c) has been taken in 2009, as well as some additional data with negative beam on nuclear targets. The spectrometer features a large angular acceptance and high momentum resolution and also good coverage by electromagnetic calorimetry, crucial for the detection of final states involving $\\pi^0$ or $\\eta$. A first important result is the observation of a significant $J^{PC}$ spin exotic signal consistent with the disputed $\\pi_1(1600)$ in the pilot run dat...

  10. Spectroscopy Made Easy: Evolution (United States)

    Piskunov, Nikolai; Valenti, Jeff A.


    Context. The Spectroscopy Made Easy (SME) package has become a popular tool for analyzing stellar spectra, often in connection with large surveys or exoplanet research. SME has evolved significantly since it was first described in 1996, but many of the original caveats and potholes still haunt users. The main drivers for this paper are complexity of the modeling task, the large user community, and the massive effort that has gone into SME. Aims: We do not intend to give a comprehensive introduction to stellar atmospheres, but will describe changes to key components of SME: the equation of state, opacities, and radiative transfer. We will describe the analysis and fitting procedure and investigate various error sources that affect inferred parameters. Methods: We review the current status of SME, emphasizing new algorithms and methods. We describe some best practices for using the package, based on lessons learned over two decades of SME usage. We present a new way to assess uncertainties in derived stellar parameters. Results: Improvements made to SME, better line data, and new model atmospheres yield more realistic stellar spectra, but in many cases systematic errors still dominate over measurement uncertainty. Future enhancements are outlined.

  11. Variable angle correlation spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Y K [Univ. of California, Berkeley, CA (United States)


    In this dissertation, a novel nuclear magnetic resonance (NMR) technique, variable angle correlation spectroscopy (VACSY) is described and demonstrated with {sup 13}C nuclei in rapidly rotating samples. These experiments focus on one of the basic problems in solid state NMR: how to extract the wealth of information contained in the anisotropic component of the NMR signal while still maintaining spectral resolution. Analysis of the anisotropic spectral patterns from poly-crystalline systems reveal information concerning molecular structure and dynamics, yet in all but the simplest of systems, the overlap of spectral patterns from chemically distinct sites renders the spectral analysis difficult if not impossible. One solution to this problem is to perform multi-dimensional experiments where the high-resolution, isotropic spectrum in one dimension is correlated with the anisotropic spectral patterns in the other dimensions. The VACSY technique incorporates the angle between the spinner axis and the static magnetic field as an experimental parameter that may be incremented during the course of the experiment to help correlate the isotropic and anisotropic components of the spectrum. The two-dimensional version of the VACSY experiments is used to extract the chemical shift anisotropy tensor values from multi-site organic molecules, study molecular dynamics in the intermediate time regime, and to examine the ordering properties of partially oriented samples. The VACSY technique is then extended to three-dimensional experiments to study slow molecular reorientations in a multi-site polymer system.

  12. Broadband transmission EPR spectroscopy.

    Directory of Open Access Journals (Sweden)

    Wilfred R Hagen

    Full Text Available EPR spectroscopy employs a resonator operating at a single microwave frequency and phase-sensitive detection using modulation of the magnetic field. The X-band spectrometer is the general standard with a frequency in the 9-10 GHz range. Most (biomolecular EPR spectra are determined by a combination of the frequency-dependent electronic Zeeman interaction and a number of frequency-independent interactions, notably, electron spin - nuclear spin interactions and electron spin - electron spin interactions, and unambiguous analysis requires data collection at different frequencies. Extant and long-standing practice is to use a different spectrometer for each frequency. We explore the alternative of replacing the narrow-band source plus single-mode resonator with a continuously tunable microwave source plus a non-resonant coaxial transmission cell in an unmodulated external field. Our source is an arbitrary wave digital signal generator producing an amplitude-modulated sinusoidal microwave in combination with a broadband amplifier for 0.8-2.7 GHz. Theory is developed for coaxial transmission with EPR detection as a function of cell dimensions and materials. We explore examples of a doublet system, a high-spin system, and an integer-spin system. Long, straigth, helical, and helico-toroidal cells are developed and tested with dilute aqueous solutions of spin label hydroxy-tempo. A detection limit of circa 5 µM HO-tempo in water at 800 MHz is obtained for the present setup, and possibilities for future improvement are discussed.

  13. Meson Spectroscopy at COMPASS

    CERN Document Server

    Grube, Boris


    The goal of the COMPASS experiment at CERN is to study the structure and dynamics of hadrons. The two-stage spectrometer used by the experiment has large acceptance and covers a wide kinematic range for charged as well as neutral particles and can therefore measure a wide range of reactions. The spectroscopy of light mesons is performed with negative (mostly $\\pi^-$) and positive ($p$, $\\pi^+$) hadron beams with a momentum of 190 GeV/$c$. The light-meson spectrum is measured in different final states produced in diffractive dissociation reactions with squared four-momentum transfer $t$ to the target between 0.1 and 1.0 $(\\text{GeV}/c)^2$. The flagship channel is the $\\pi^-\\pi^-\\pi^+$ final state, for which COMPASS has recorded the currently world's largest data sample. These data not only allow to measure the properties of known resonances with high precision, but also to observe new states. Among these is a new axial-vector signal, the $a_1(1420)$, with unusual properties. Novel analysis techniques have been...

  14. Laser spectroscopy of radium

    Energy Technology Data Exchange (ETDEWEB)

    Santra, Bodhaditya; Dammalapati, Umakanth; Jungmann, Klaus; Willmann, Lorenz [KVI, University of Groningen (Netherlands)


    Searches for permanent electric dipole moments (EDMs) of fundamental particles are sensitive probes of physics beyond the Standard Model. Fundamental EDMs can experience enhancements in atomic and molecular systems. In particular, isotopes of the heavy alkaline earth element radium exhibit the largest known enhancement factors for any atomic systems due to their atomic and nuclear structure. A sensitive search for EDMs will require an efficient use of the rare isotopes, which are available from radioactive sources or at rare isotope facilities like TRI{mu}P at KVI. Here, laser cooling and trapping methods play a crucial role. The main transitions from the ground state have been identified by laser spectroscopy. Nevertheless, the strongest cooling transitions 7s{sup 2} {sup 1}S{sub 0}-7s7p {sup 1}P{sub 1} suffers from strong leakage to metastable states, similar to the case of barium. We describe the experimental approach to determine the wavelength of the three needed repump transitions, which then will permit an efficient capture of radium atoms into a magneto optical trap.

  15. Meson spectroscopy at COMPASS

    Directory of Open Access Journals (Sweden)

    Grube Boris


    Full Text Available The goal of the COMPASS experiment at CERN is to study the structure and dynamics of hadrons. The two-stage spectrometer used by the experiment has large acceptance and covers a wide kinematic range for charged as well as neutral particles and can therefore measure a wide range of reactions. The spectroscopy of light mesons is performed with negative (mostly π− and positive (p, π+ hadron beams with a momentum of 190 GeV/c. The light-meson spectrum is measured in different final states produced in diffractive dissociation reactions with squared four-momentum transfer t to the target between 0.1 and 1.0 (GeV=c2. The flagship channel is the π−π−π+ final state, for which COMPASS has recorded the currently world’s largest data sample. These data not only allow to measure the properties of known resonances with high precision, but also to observe new states. Among these is a new axial-vector signal, the a1(1420, with unusual properties. Novel analysis techniques have been developed to extract also the amplitude of the π−π+ subsystem as a function of 3π mass from the data. The findings are confirmed by the analysis of the π−π0π0 final state.

  16. Meson Spectroscopy at COMPASS

    CERN Document Server

    Grube, Boris


    The COmmon Muon and Proton Apparatus for Structure and Spectroscopy (COMPASS) is a multi-purpose fixed-target experiment at the CERN Super Proton Synchrotron (SPS) aimed at studying the structure and spectrum of hadrons. The two-stage spectrometer has a good acceptance for charged as well as neutral particles over a wide kinematic range and thus allows to access a wide range of reactions. Light mesons are studied with negative (mostly $\\pi^-$) and positive ($p$, $\\pi^+$) hadron beams with a momentum of 190 GeV/$c$. The spectrum of light mesons is investigated in various final states produced in diffractive dissociation reactions at squared four-momentum transfers to the target between 0.1 and 1.0 $(\\text{GeV}/c)^2$. The flagship channel is the $\\pi^-\\pi^+\\pi^-$ final state, for which COMPASS has recorded the currently largest data sample. These data not only allow to measure the properties of known resonances with high precision, but also to search for new states. Among these is a new resonance-like signal, t...

  17. Hadron Spectroscopy in COMPASS

    CERN Document Server

    Grube, Boris


    The COmmon Muon and Proton Apparatus for Structure and Spectroscopy (COMPASS) is a multi-purpose fixed-target experiment at the CERN Super Proton Synchrotron (SPS) aimed at studying the structure and spectrum of hadrons. In the naive Constituent Quark Model (CQM) mesons are bound states of quarks and antiquarks. QCD, however, predict the existence of hadrons beyond the CQM with exotic properties interpreted as excited glue (hybrids) or even pure gluonic bound states (glueballs). One main goal of COMPASS is to search for these states. Particularly interesting are so called spin-exotic mesons which have J^{PC} quantum numbers forbidden for ordinary q\\bar{q} states. Its large acceptance, high resolution, and high-rate capability make the COMPASS experiment an excellent device to study the spectrum of light-quark mesons in diffractive and central production reactions up to masses of about 2.5 GeV. COMPASS is able to measure final states with charged as well as neutral particles, so that resonances can be studied ...

  18. Meson spectroscopy at COMPASS (United States)

    Grube, Boris


    The goal of the COMPASS experiment at CERN is to study the structure and dynamics of hadrons. The two-stage spectrometer used by the experiment has large acceptance and covers a wide kinematic range for charged as well as neutral particles and can therefore measure a wide range of reactions. The spectroscopy of light mesons is performed with negative (mostly π-) and positive (p, π+) hadron beams with a momentum of 190 GeV/c. The light-meson spectrum is measured in different final states produced in diffractive dissociation reactions with squared four-momentum transfer t to the target between 0.1 and 1.0 (GeV=c)2. The flagship channel is the π-π-π+ final state, for which COMPASS has recorded the currently world's largest data sample. These data not only allow to measure the properties of known resonances with high precision, but also to observe new states. Among these is a new axial-vector signal, the a1(1420), with unusual properties. Novel analysis techniques have been developed to extract also the amplitude of the π-π+ subsystem as a function of 3π mass from the data. The findings are confirmed by the analysis of the π-π0π0 final state.

  19. Beta-blockers and heart failure. (United States)

    Cruickshank, John M


    The life-time risk of developing HF is about 20% (40% if hypertension present). With increasing longevity in the developed world the burden of HF (hospitalisation) is set to increase over the next 10-20 years. CAD and hypertension are the two main causes of HF; CAD (and obesity) in the case of systolic HF and hypertension in the case of diastolic HF (mainly in the elderly). BB have become the corner-stone (alongside ACE-inhibitors) in the treatment of systolic HF. Bisoprolol, metoprolol and carvedilol (on an ACE-inhibitor background) have reduced all-cause death by 34-5%. The presence of intrinsic sympathomemetic activity (xamoterol, bucindolol, nebivolol) diminishes efficacy in the treatment of systolic HF. First-line bisoprolol has proved "non-inferior" to first-line enalapril in reducing all-cause death and is probably superior in reducing sudden death. The main mode of action of BB in treating systolic HF is inhibition of chronic beta-1 stimulation-induced myocardial apoptosis/necrosis/inflammation. The combination of pure beta-1 blockade (low-dose bisoprolol) and pure beta-2 blockade (clenbuterol) may prove invaluable in the treatment of end-stage systolic HF (thus avoiding cardiac transplantation). The appropriate treatment of diastolic HF has yet to be determined. Beta-blockade is effective in the prevention of HF i) in the post-MI period and ii) as first-line agents in the treatment of young/middle-aged hypertension and as second-line agents (to first-line diuretics) in the treatment of elderly systolic hypertension. BB are highly effective in reversing LVH in young/middle-aged hypertensives (LVH pre-disposes to HF in young/middle-aged hypertension) and are (bisoprolol) at least as good as ACE-inhibitors. Choice of BB is important as benefit is not a class-effect. ISA (xamoterol, bucindolol, nebivolol) markedly diminishes efficacy. The choice is between bisoprolol, metoprolol succinate and carvedilol for optimal efficacy. Adverse reactions are associated

  20. Study of {sup 193}Os beta{sup -} decay; Estudo do decaimento beta{sup -} do {sup 193}Os

    Energy Technology Data Exchange (ETDEWEB)

    Zahn, Guilherme Soares


    In this work, the excited levels of {sup 193}Ir populated by the beta{sup -} decay of {sup 193}Os (T{sub 1/2} {approx} 30h) were investigated. For that purpose, {approx} 5 mg samples of 99%-enriched {sup 192}Os were irradiated under a thermal neutron flux of {approx} 10{sup 12} s{sup -1} and then analysed both using single gamma spectroscopy and a 4-detector multi parametric acquisition facility, which provided data for both a gamma gamma coincidence analysis and a directional angular correlation gamma gamma ({theta} ) study. From these data, 28 transitions were added to this decay scheme, 11 of which were previously known from nuclear reactions and 17 observed for the first time. Eight excited levels were also added to the decay scheme, 3 of which were known from nuclear reaction studies - the remaining 5 are suggested for the first time. Moreover, it was possible to confirm suspicions found in reference that the levels at 848.93 keV and 849.093 keV are indeed the same; it was also possible to confirm the existence of an excited level at 806.9 keV, which had been inferred, but not experimentally confirmed in beta decay studies to date. The angular correlation analysis allowed for the definition of the spin of the excited level at 874 keV as 5/2{sup +;} moreover, the results showed a 79% probability that the spin of the 1078 keV level is 5/2/'-, and also restricted the spin possibilities for the new excited level at 960 keV to two values (1/2 or 3/2). It was also possible to measure the multipolarity mixing ratio ({delta}{sub Ln+1}/L{sub n}) for 43 transitions - 19 of them for the first time and most of the others with a better precision than previously known. Finally, an attempt was made to understand the low-lying levels structure for this nucleus using a theoretical model, which reproduced the ground state and the two lowest-lying excited levels in {sup 193}Ir. (author)