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Sample records for belted rabbits infected

  1. Viral infections of rabbits.

    Science.gov (United States)

    Kerr, Peter J; Donnelly, Thomas M

    2013-05-01

    Viral diseases of rabbits have been used historically to study oncogenesis (e.g. rabbit fibroma virus, cottontail rabbit papillomavirus) and biologically to control feral rabbit populations (e.g. myxoma virus). However, clinicians seeing pet rabbits in North America infrequently encounter viral diseases although myxomatosis may be seen occasionally. The situation is different in Europe and Australia, where myxomatosis and rabbit hemorrhagic disease are endemic. Advances in epidemiology and virology have led to detection of other lapine viruses that are now recognized as agents of emerging infectious diseases. Rabbit caliciviruses, related to rabbit hemorrhagic disease, are generally avirulent, but lethal variants are being identified in Europe and North America. Enteric viruses including lapine rotavirus, rabbit enteric coronavirus and rabbit astrovirus are being acknowledged as contributors to the multifactorial enteritis complex of juvenile rabbits. Three avirulent leporid herpesviruses are found in domestic rabbits. A fourth highly pathogenic virus designated leporid herpesvirus 4 has been described in Canada and Alaska. This review considers viruses affecting rabbits by their clinical significance. Viruses of major and minor clinical significance are described, and viruses of laboratory significance are mentioned.

  2. Parasitic infections of wild rabbits and hares

    Directory of Open Access Journals (Sweden)

    Ilić Tamara

    2014-01-01

    Full Text Available The paper presents the most important parasitic infections of wild rabbits and hares, which harmful effect in this animal population is manifested as a gradual weakening of the immune system, reduction in fertility, weight loss and constant exhaustion. Order of Lagomorpha (hares or lagomorphs belongs to superorder of higher mammals which includes the family of rabbits (Leporidae which are represented in Europe as well as the family of whistleblowers (Ochotonidae which live only in North America and Northern regions of Asia. The most important representatives of Leporidae family are European hare (Lepus europeus and wild rabbit (Oryctolagus cuniculus. The most important endoparasitosis of hares and wild rabbits are: coccidiosis, encephalitozoonosis (nosemosis, toxoplasmosis, sarcocystosis, giardiasis, cryptosporidiosis, protostrongylosis, trichostrngylodosis, passalurosis, anoplocephalidosis, cysticercosis and fasciolosis. The most frequent ectoparasites of rabbits and wild hares are fleas, lice and ticks. Reduction in hare population, which is noticed in whole Europe including Serbia, is caused by changed living conditions, quantitatively and qualitatively insufficient nutrition, increased use of herbicides as well as various infectious diseases and the diseases of parasitic etiology. Since wild rabbits and hares pose a threat to health of domestic rabbits and people, knowledge of parasitic fauna of these wild animals is of extreme epizootiological and epidemiological importance.

  3. Is there a difference between hare syphilis and rabbit syphilis? Cross infection experiments between rabbits and hares

    NARCIS (Netherlands)

    Lumeij, J.T.; Mikalová, L.; Smajs, D.

    2013-01-01

    Abstract Cross infection of rabbits and hares with Treponema paraluiscuniculi from rabbits and the related microorganism from hares, which was provisionally named "Treponema paraluisleporis", revealed that T. paraluiscuniculi affects rabbits clinically, but only causes seroconversion in hares withou

  4. Experimental infection of wild-caught European rabbits (Oryctolagus cuniculus) with Sarcoptes scabiei from a naturally infected wild rabbit.

    Science.gov (United States)

    Millán, J; Casais, R; Colomar, V; Bach, E; Prieto, J M; Velarde, R

    2013-06-01

    Scabies was recently reported for the first time in the European wild rabbit, Oryctolagus cuniculus (Lagomorpha: Leporidae). We experimentally exposed 10 seronegative wild-caught rabbits to skin from a mangy wild rabbit. Serological, physiological, parasitological and histopathological changes were recorded. Three rabbits developed antibodies at 2-5 weeks post-infection (w.p.i.), two of which then developed lesions at 7 w.p.i. One of these had a small area of alopecia on the hind limb that healed naturally within 1 week; the other developed more extensive lesions restricted to the hind limbs (as typically observed in wild rabbits) that lasted until the rabbit died (12.5 w.p.i.). The third rabbit died of trauma 5 w.p.i. before developing any lesions. Antibodies in the healed rabbit disappeared from serum at 8 w.p.i., whereas antibody levels in the sick rabbit increased until its death. Disseminated intravascular coagulation and hepatic necrosis, probably arising from a concomitant infection with rabbit haemorrhagic disease virus, were the likely final cause of death in this rabbit. The mangy rabbit that served as a donor died of a multifocal fibrinosuppurative pneumonia that may have been secondary to the skin bacterial pyoderma.

  5. SPF rabbits infected with rabbit hepatitis E virus isolate experimentally showing the chronicity of hepatitis.

    Science.gov (United States)

    Han, Jian; Lei, Yaxin; Liu, Lin; Liu, Peng; Xia, Junke; Zhang, Yulin; Zeng, Hang; Wang, Lin; Wang, Ling; Zhuang, Hui

    2014-01-01

    This study focused on investigating the pathogenesis seen in specific-pathogen-free (SPF) rabbits following infection with a homologous rabbit HEV isolate (CHN-BJ-rb14) and comparing it to that seen following infection with a heterologous swine genotype 4 HEV isolate (CHN-XJ-SW13). Three of the four animals inoculated with the homologous rabbit HEV became infected, exhibiting an intermittent viremia, obvious fluctuations of liver function biomarkers alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and persistent fecal virus shedding throughout the nine month study. In addition, liver histopathology showed both chronic inflammation and some degree of fibrosis. Both positive and negative-stranded HEV RNA and HEV antigen expression were detected in liver, brain, stomach, duodenum and kidney from the necropsied rabbits. Inflammation of extrahepatic tissue (duodenum and kidney) was also observed. Three of the four rabbits inoculated with the heterologous genotype 4 swine HEV also became infected, showing similar levels of anti-HEV antibody to that generated following infection with the homologous virus isolate. The duration of both viremia and fecal shedding of virus was however shorter following infection with the heterologous virus and there was no significant elevation of liver function biomarkers. These results suggest that rabbit HEV infection may cause more severe hepatitis and prolong the course of the disease, with a possible chronic trend of hepatitis in SPF rabbits.

  6. Detection and localization of rabbit hepatitis e virus and antigen in systemic tissues from experimentally intraperitoneally infected rabbits.

    Science.gov (United States)

    Mao, Jingjing; Zhao, Yue; She, Ruiping; Cao, Binbin; Xiao, Peng; Wu, Qiaoxing; Guo, Zhaojie; Ma, Longhuan; Soomro, Majid Hussain

    2014-01-01

    Rabbit hepatitis E virus (HEV) is a novel genotype of HEV, and is considered to pose a risk of zoonotic transmission. Research into the systemic distribution of rabbit HEV in rabbits during different periods of infection has rarely been reported. To better understand this virus, we infected rabbits with second-passage rabbit HEV via an intraperitoneal route. After inoculation, the infection showed two types, temporary and constant infection. The detection of HEV RNA in the feces varied with time, and serum antigen correlated with fecal HEV RNA. Viremia only appeared 72 days after inoculation. The rabbits remained antibody negative throughout the experimental period. When HEV was localized, several organs besides the liver were HEV RNA positive. Tissue antigen was observed immunohistochemically in the different cells of various organs, especially in parts of the small intestine and the characteristic rabbit gut-associated lymphoid tissue. These data provide valuable information for future research into the pathogenesis of HEV.

  7. Pathology and Therapy in Naturally Eimeria stiedae-Infected Rabbits

    OpenAIRE

    Singla, L. D.; Juyal, P. D.; B S Sandhu

    2000-01-01

    Clinical hepatic coccidiosis in New Zealand white rabbits (n=24) of either sex (aged 1-2month) with clinical signs, pathology an therapy is presented. The infected rabbits showedanorexia, reluctance to move and death within 3-4 days. Liver showed irregular whitish nodulesscattered on its surface and in deeper parenchyma. Characteristic histopathologjcal changes inliver with different developmental stages of Eimeria stiedae in the epithelial cells of bile ductshave been recorded. Treatment of ...

  8. The rabbit as an infection model for equine proliferative enteropathy.

    Science.gov (United States)

    Sampieri, Francesca; Allen, Andrew L; Pusterla, Nicola; Vannucci, Fabio A; Antonopoulos, Aphroditi J; Ball, Katherine R; Thompson, Julie; Dowling, Patricia M; Hamilton, Don L; Gebhart, Connie J

    2013-04-01

    The objective of this study was to demonstrate the susceptibility of rabbits to Lawsonia intracellularis obtained from a case of clinical equine proliferative enteropathy (EPE). This is a preliminary step toward developing a rabbit infection model for studying pathogenesis and therapy of EPE in horses. Nine does were equally assigned to 3 groups. Animals in 2 groups (Group 1 and Group 2) were orally inoculated with different doses of cell-cultured L. intracellularis. Controls (Group 3) were sham-inoculated. Feces and blood were collected before the rabbits were infected and at 7, 14, and 21 days post-infection (DPI). Serum immunoglobulin G (IgG) titers were measured using an immunoperoxidase monolayer assay (IPMA) and fecal samples were analyzed with quantitative polymerase chain reaction (qPCR). A doe from each group was euthanized at 7, 14, and 21 DPI for collection and evaluation of intestinal samples. Tissues were stained by routine hematoxylin and eosin (H&E) method and immunohistochemistry (IHC) with L. intracellularis-specific mouse monoclonal antibody. At 14 DPI, serologic responses were detected in both infected groups, which maintained high titers through to 21 DPI. Lawsonia intracellularis DNA was detected in the feces of Group 2 on 7 DPI and in both infected groups on 14 DPI. Gross lesions were apparent in Group 1 and Group 2 on 14 DPI. Immunohistochemistry confirmed L. intracellularis antigen within cells of rabbits in Group 1 and Group 2 on 7, 14, and 21 DPI. No lesions, serologic response, shedding, or IHC labeling were found in Group 3 rabbits. This study describes an EPE rabbit model that simulates natural infection, as typical lesions, immune response, and fecal shedding were present.

  9. The rabbit as an infection model for equine proliferative enteropathy

    Science.gov (United States)

    Sampieri, Francesca; Allen, Andrew L.; Pusterla, Nicola; Vannucci, Fabio A.; Antonopoulos, Aphroditi J.; Ball, Katherine R.; Thompson, Julie; Dowling, Patricia M.; Hamilton, Don L.; Gebhart, Connie J.

    2013-01-01

    The objective of this study was to demonstrate the susceptibility of rabbits to Lawsonia intracellularis obtained from a case of clinical equine proliferative enteropathy (EPE). This is a preliminary step toward developing a rabbit infection model for studying pathogenesis and therapy of EPE in horses. Nine does were equally assigned to 3 groups. Animals in 2 groups (Group 1 and Group 2) were orally inoculated with different doses of cell-cultured L. intracellularis. Controls (Group 3) were sham-inoculated. Feces and blood were collected before the rabbits were infected and at 7, 14, and 21 days post-infection (DPI). Serum immunoglobulin G (IgG) titers were measured using an immunoperoxidase monolayer assay (IPMA) and fecal samples were analyzed with quantitative polymerase chain reaction (qPCR). A doe from each group was euthanized at 7, 14, and 21 DPI for collection and evaluation of intestinal samples. Tissues were stained by routine hematoxylin and eosin (H&E) method and immunohistochemistry (IHC) with L. intracellularis-specific mouse monoclonal antibody. At 14 DPI, serologic responses were detected in both infected groups, which maintained high titers through to 21 DPI. Lawsonia intracellularis DNA was detected in the feces of Group 2 on 7 DPI and in both infected groups on 14 DPI. Gross lesions were apparent in Group 1 and Group 2 on 14 DPI. Immunohistochemistry confirmed L. intracellularis antigen within cells of rabbits in Group 1 and Group 2 on 7, 14, and 21 DPI. No lesions, serologic response, shedding, or IHC labeling were found in Group 3 rabbits. This study describes an EPE rabbit model that simulates natural infection, as typical lesions, immune response, and fecal shedding were present. PMID:24082402

  10. Detection of L1, infectious virions and anti-L1 antibody in domestic rabbits infected with cottontail rabbit papillomavirus.

    Science.gov (United States)

    Hu, Jiafen; Budgeon, Lynn R; Cladel, Nancy M; Culp, Timothy D; Balogh, Karla K; Christensen, Neil D

    2007-12-01

    Shope papillomavirus or cottontail rabbit papillomavirus (CRPV) is one of the first small DNA tumour viruses to be characterized. Although the natural host for CRPV is the cottontail rabbit (Sylvilagus floridanus), CRPV can infect domestic laboratory rabbits (Oryctolagus cuniculus) and induce tumour outgrowth and cancer development. In previous studies, investigators attempted to passage CRPV in domestic rabbits, but achieved very limited success, leading to the suggestion that CRPV infection in domestic rabbits was abortive. The persistence of specific anti-L1 antibody in sera from rabbits infected with either virus or viral DNA led us to revisit the questions as to whether L1 and infectious CRPV can be produced in domestic rabbit tissues. We detected various levels of L1 protein in most papillomas from CRPV-infected rabbits using recently developed monoclonal antibodies. Sensitive in vitro infectivity assays additionally confirmed that extracts from these papillomas were infectious. These studies demonstrated that the CRPV/New Zealand White rabbit model could be used as an in vivo model to study natural virus infection and viral life cycle of CRPV and not be limited to studies on abortive infections.

  11. Apoptosis of peripheral blood leukocytes from rabbits infected with non-haemagglutinating strains of rabbit haemorrhagic disease virus (RHDV).

    Science.gov (United States)

    Niedźwiedzka-Rystwej, Paulina; Deptuła, Wiesław

    2012-09-15

    The report demonstrates that the induction of apoptosis in peripheral blood granulocytes and lymphocytes of rabbits infected with three non-haemagglutinating RHDV strains (English Rainham, German Frankfurt, and Spanish Asturias) is a crucial determinant of the pathogenesis of rabbit haemorrhagic disease. Apoptosis was measured by flow cytometric detection of caspase activity. These studies demonstrated that the investigated RHDV (rabbit haemorrhagic disease virus) viral strains affected leukocyte apoptosis to varying degrees. Enhanced leukocyte apoptosis was detected between 4 and 36 h after infection and was more pronounced in lymphocytes than in granulocytes. The data presented here thus provide a preliminary understanding of the kinetics of apoptosis in leukocytes of rabbits infected with RHDV.

  12. Apoptosis of peripheral blood leucocytes in rabbits infected with different strains of rabbit haemorrhagic disease virus.

    Science.gov (United States)

    Niedźwiedzka-Rystwej, Paulina; Hukowska-Szematowicz, Beata; Tokarz-Deptuła, Beata; Trzeciak-Ryczek, Alicja; Działo, Joanna; Deptuła, Wiesław

    2013-01-01

    The pathogenicity of RHDV (rabbit haemorrhagic disease virus) is mainly associated with its affinity to blood vessels, with causing disseminated intravascular coagulations (DIC), and with the stimulation of the host immune system. Moreover, there are implications suggesting that apoptosis may be a pivotal process in understanding the basis of viral haemorrhagic disease in rabbits - a serious infectious disease causing mortality to wild and domestic rabbits. The aim of this study is to evaluate, by means of flow cytometry, the dynamics of apoptosis in peripheral blood granulocytes and lymphocytes in rabbits experimentally infected with seven different strains of RHDV and so-called antigenic variants of RHDV denominated as RHDVa, i.e.: Hungarian 24V/89, 1447V/96, 72V/2003; Austrian 01-04, 237/04, V-412 and French 05-01. The results showed that all of the RHDV and RHDVa strains cause an increase in the number of apoptotic cells throughout the infection, which might indicate the need for further analysis of the importance of this process.

  13. Streptococcus agalactiae infection in domestic rabbits, Oryctolagus cuniculus.

    Science.gov (United States)

    Ren, S Y; Geng, Y; Wang, K Y; Zhou, Z Y; Liu, X X; He, M; Peng, X; Wu, C Y; Lai, W M

    2014-12-01

    Streptococcus agalactiae (Group B streptococcus, GBS) has emerged as an important pathogen that affects humans and animals, including aquatic species. In August 2011, a severe infectious disease affecting rabbits, which caused 42% mortality, occurred in Mianyang, Sichuan Province, China. The main clinical signs included acute respiratory distress syndrome, fever, paddling and convulsions. A Gram-positive, chain-forming coccus was isolated from the primary organs and tissues of diseased rabbits and then identified as S. agalactiae by morphology, biochemical and physiological characteristics, 16S rDNA and gyrB gene sequences analysis. All isolates of S. agalactiae showed a similar antibiotic susceptibility, which were sensitive to florfenicol, ampicillin,gentamicin and norfloxacin, as well as being resistant to penicillin, amoxicillin and tetracycline. To our knowledge, this is the first report on S. agalactiae natural infection in domestic rabbits.

  14. Rabbit haemorrhagic disease: are Australian rabbits (Oryctolagus cuniculus) evolving resistance to infection with Czech CAPM 351 RHDV?

    Science.gov (United States)

    Elsworth, P G; Kovaliski, J; Cooke, B D

    2012-11-01

    Rabbit haemorrhagic disease is a major tool for the management of introduced, wild rabbits in Australia. However, new evidence suggests that rabbits may be developing resistance to the disease. Rabbits sourced from wild populations in central and southeastern Australia, and domestic rabbits for comparison, were experimentally challenged with a low 60 ID50 oral dose of commercially available Czech CAPM 351 virus - the original strain released in Australia. Levels of resistance to infection were generally higher than for unselected domestic rabbits and also differed (0-73% infection rates) between wild populations. Resistance was lower in populations from cooler, wetter regions and also low in arid regions with the highest resistance seen within zones of moderate rainfall. These findings suggest the external influences of non-pathogenic calicivirus in cooler, wetter areas and poor recruitment in arid populations may influence the development rate of resistance in Australia.

  15. Coccidian and nematode infections influence prevalence of antibody to myxoma and rabbit hemorrhagic disease viruses in European rabbits.

    Science.gov (United States)

    Bertó-Moran, Alejandro; Pacios, Isabel; Serrano, Emmanuel; Moreno, Sacramento; Rouco, Carlos

    2013-01-01

    The interaction among several parasites in European rabbits (Oryctolagus cuniculus) is crucial to host fitness and to the epidemiology of myxomatosis and rabbit hemorrhagic disease. These diseases have caused significant reductions in rabbit populations on the Iberian Peninsula. Most studies have focused on the epidemiology and pathogenesis of these viruses individually, and little is known about interactions between these viruses and other parasites. Taking advantage of an experimental restocking program in Spain, the effects of coccidian and nematode infections on the probability of having detectable antibody to myxoma and rabbit hemorrhagic disease viruses were tested in European wild rabbits. For 14 mo, we monitored rabbit abundance and parasite loads (coccidia and nematodes) in three reintroduced rabbit populations. While coccidian and nematode loads explained seasonal antibody prevalences to myxoma virus, the pattern was less clear for rabbit hemorrhagic disease. Contrary to expectations, prevalence of antibody to myxoma virus was inversely proportional to coccidian load, while nematode load seemed to play a minor role. These results have implications for viral disease epidemiology and for disease management intended to increase rabbit populations in areas where they are important for ecosystem conservation.

  16. Prevention and treatment of Encephalitozoon cuniculi infection in immunosuppressed rabbits with fenbendazole.

    Science.gov (United States)

    Abu-Akkada, S S; Oda, S S

    2016-01-01

    This study was conducted to evaluate the efficacy of oral administration of fenbendazole (20 mg/kg body weight) prior to and after experimental infection of immunosuppressed rabbits with Encephalitozoon cuniculi. A total of thirty rabbits were divided into five groups: NN (non-immunosuppressed; non-infected), IN (immunosuppressed; non-infected), IPI (immunosuppressed; protected-infected), ITI (immunosuppressed; treated-infected), and II (immunosuppressed; infected) groups. Fenbendazole was administered as a prophylactic for seven successive days before infection with E. cuniculi and as a treatment for four weeks initiated on the 28th day post-challenge (PC). Experimental rabbits were infected with intraperitoneal injection of 2 × 10(5)E. cuniculi spores. Parameters evaluated were body weight, detection of spores in urine, serum antibody assay, hematological, biochemical and histopathological changes. The IPI and ITI groups showed a significant better final bwt than the II group. Spores were detected in urine of all infected rabbits from the 28th day PC until the end of the study. The IPI group showed the least values of antibodies (IgG) compared to the ITI and II groups. Concerning histopathological changes, the intensity of the lesions was marked particularly in the II rabbits and to a lesser extent in the ITI rabbits. Noticeable improvement was found in the IPI rabbits. It could be concluded that fenbendazole was effective to some extent in protection of rabbits against E. cuniculi infection, while when administered as a therapeutic no significant effects were observed.

  17. Prevention and treatment of Encephalitozoon cuniculi infection in immunosuppressed rabbits with fenbendazole

    Science.gov (United States)

    Abu-Akkada, S. S.; Oda, S. S.

    2016-01-01

    This study was conducted to evaluate the efficacy of oral administration of fenbendazole (20 mg/kg body weight) prior to and after experimental infection of immunosuppressed rabbits with Encephalitozoon cuniculi. A total of thirty rabbits were divided into five groups: NN (non-immunosuppressed; non-infected), IN (immunosuppressed; non-infected), IPI (immunosuppressed; protected-infected), ITI (immunosuppressed; treated-infected), and II (immunosuppressed; infected) groups. Fenbendazole was administered as a prophylactic for seven successive days before infection with E. cuniculi and as a treatment for four weeks initiated on the 28th day post-challenge (PC). Experimental rabbits were infected with intraperitoneal injection of 2 × 105 E. cuniculi spores. Parameters evaluated were body weight, detection of spores in urine, serum antibody assay, hematological, biochemical and histopathological changes. The IPI and ITI groups showed a significant better final bwt than the II group. Spores were detected in urine of all infected rabbits from the 28th day PC until the end of the study. The IPI group showed the least values of antibodies (IgG) compared to the ITI and II groups. Concerning histopathological changes, the intensity of the lesions was marked particularly in the II rabbits and to a lesser extent in the ITI rabbits. Noticeable improvement was found in the IPI rabbits. It could be concluded that fenbendazole was effective to some extent in protection of rabbits against E. cuniculi infection, while when administered as a therapeutic no significant effects were observed. PMID:27822234

  18. Serological survey of hepatitis E virus infection in farmed and pet rabbits in Italy.

    Science.gov (United States)

    Di Bartolo, Ilaria; De Sabato, L; Marata, A; Martinelli, N; Magistrali, C F; Monini, M; Ponterio, E; Ostanello, F; Ruggeri, F M

    2016-05-01

    The recent identification in rabbits of hepatitis E viruses (HEV) related to viruses infecting humans raises the question of the role of this species as possible HEV reservoir. A serological survey on rabbit HEV infection was conducted in Italy during 2013-2014, including both farmed and pet rabbits. We found an anti-HEV antibody seroprevalence of 3.40 % in 206 farmed rabbits (collected on 7 farms) and 6.56 % in 122 pets. RNA was extracted from IgG-positive sera and analyzed by HEV-specific real-time RT-PCR. None of the samples were positive, confirming that no viremia was present in the presence of IgG. Only one serum sample from a farmed rabbit was positive for IgM, but no HEV RNA was detected in it. Pet rabbit feces were also tested for HEV RNA, with negative results. This finding suggests that HEV is circulating in rabbits in Italy.

  19. Histopathological features of Capillaria hepatica infection in laboratory rabbits.

    Science.gov (United States)

    Mowat, Vasanthi; Turton, John; Stewart, Jacqui; Lui, Kai Chiu; Pilling, Andrew M

    2009-08-01

    Capillaria hepatica is a nematode parasite of wild rodents and other mammals. Adult worms inhabit the liver. Recently, during the necropsy examination of a group of 160 rabbits from a commercial supplier, firm pale or cystic areas (1-5 mm) were noted on the liver in thirteen animals. On further investigation, these animals were found to be infected with C. hepatica. The histopathological features of the infection in the rabbit are described for the first time and diagnostic features recorded. Lesions were identified predominantly in portal tracts consisting of dilated bile ducts with luminal debris, peribiliary inflammatory cell infiltrates, and fibrosis. Large granulomas (macrogranulomas) were evident in portal areas and involved the bile ducts. Macrogranulomas contained collections of characteristic C. hepatica eggs, macrophages, eosinophils, and lymphocytes. Small granulomas (microgranulomas), characterized by epithelioid macrophages surrounded by lymphocytes and eosinophils, were also identified. C. hepatica eggs were also observed in the lumina of the bile ducts and gall bladder. No adult C. hepatica worms were identified. Oocysts of Eimeria stiedae were also evident in the biliary epithelium in some animals. The unique characteristics of the C. hepatica life cycle are described, and the differential diagnosis of hepatic capillariasis is discussed.

  20. Congenital infection of rabbits with Schistosoma japonicum and protective immunity of offspring

    Institute of Scientific and Technical Information of China (English)

    SHI You-en 石佑恩; Abdel-Moneim M Salim 沙里模; NING Chang-xiu 宁长修; GAN Yan 甘燕; ZHU Xiao-hua 朱晓华; PU Ling-yi 卜玲毅

    2004-01-01

    Background Recently congenital infection with Schistosoma japonicum (S. Japonicum) has been domonstrated in pigs, rabbits, mice and dogs. We explored the rabbit as an animal model for the congenital infection of schistosomiasis japonica and assessed the effect of a congenital S. Japonicum infection on the resistance of rabbit kittens to a postnatal challenge infection.Methods Sixteen pregnant New Zealand white rabbits were infected with a single dose of S. Japonicum cercariae. The exposed animals were divided into three groups according to the gestation age at the time of infection. Diagnosis of prenatally acquired S. Japonicum infection in the rabbit kittens was primarily based on serological tests in combination with parasitological and histopathological findings. Congenitally infected kittens were challenged percutaneously with 100 S. Japonicum cercariae to assess the effect of a congenital S. Japonicum infection on kitten resistance to a postnatal challenge infection.Conclusions This study demonstrates the possibility of congenital infection of S. Japonicum in rabbits and the resistance of congenitally infected kittens to a postnatal challenge infection. These results have important implications not only for epidemiological investigations, but also in designing government control programs for schistosomiasis.

  1. A new recombinant Orf virus (ORFV, Parapoxvirus) protects rabbits against lethal infection with rabbit hemorrhagic disease virus (RHDV).

    Science.gov (United States)

    Rohde, Joerg; Schirrmeier, Horst; Granzow, Harald; Rziha, Hanns-Joachim

    2011-11-15

    This report describes the generation of a new recombinant Orf virus (ORFV; Parapoxvirus) expressing the major capsid protein VP1 (VP60) of the calicivirus, rabbit hemorrhagic disease virus (RHDV). Authentic expression of VP1 could be demonstrated in cells infected with the recombinant D1701-V-VP1 without the need for production of infectious ORFV progeny. Notably, infected cells also released empty calicivirus-like particles (VLPs). Challenge experiments showed that even a single immunization with ≥10(5) PFU of D1701-V-VP1 protected rabbits against lethal RHDV infection. ELISA tests indicated that the protective immunity mediated by D1701-V-VP1 did not strictly depend on the presence of detectable RHDV-specific serum antibodies. The induction of interleukin-2 found only in the sera of rabbits immunized with the D1701-V-VP1, but not in sera of rabbits immunized with the inactivated commercial vaccine RIKA-VACC, might indicate also some involvement of T-cells in protection. Collectively, this work adds another example of the successful use of the ORFV vector system for the generation of a recombinant vaccine, and demonstrates its potential as an alternative vaccine to protect rabbits against RHDV infection.

  2. Phagocytosis of neutrophils in rabbits infected with antigenic variants of RHD (rabbit haemorrhagic disease) virus.

    Science.gov (United States)

    Niedźwiedzka-Rystwej, P; Deptuła, W

    2012-01-01

    The present study was aimed at determining changes in chosen elements of phagocytosis in rabbits infected with 3 antigenic variants of RHD - Hartmannsdorf, Pv97 and 9905, which differed in haemagglutination ability. The animals were tested for phagocytosis parameters, and the results revealed that the examined strains showed the differences. These variations regarded mainly Pv97 strain, as the intensity of the changes were 5 times stronger in comparison to strain Hartmannsdorf and 9905. As all of the strains examined are signified as antigenic variants, we have stated that this feature does not determine their immunological picture. The results suggest the existence of immunological dissimilarities among strains of the RHD virus, which was revealed for the first time in antigenic variants.

  3. [Dynamics of endoparasite infections in rabbits at different rearing regimes].

    Science.gov (United States)

    Nosal, Paweł; Kowal, Jerzy; Nowosad, Bogusław; Bieniek, Józef; Kowalska, Dorota

    2009-01-01

    This study was intended to evaluate the occurrence and seasonality of infection of coccidian and helminth parasites, in three rabbit warrens differently managed. Mothers stayed with their offspring on deep litter (farm A, 90 dams), in boxes cleaned weekly (farm B, 30 dams) or on slatted floor (farm C, 10 females), whereas all the young after weaning (1 to 5 months of age) were kept in cages with slatted floor. Animals were fed a complete balanced pelleted feed (warrens A and B) or traditional forage (herd C). Antiparasitic treatment involved coccidiostatic drugs. Robenidine (alternately with Lerbec in farm A) was added to food in the first two farms, whilst Sulfatyf mixed with water was given once after weaning in farm C. A total of 203 individual faecal samples from females of breeding stock, and 133 pooled samples from young animals, were collected on an annual cycle during 2007-2008 (February-January), and analyzed according to a modified concentration McMaster method. Coccidians were identified based on sporulated oocysts. A number of coccidian species (Eimeria perforans, E. media, E. magna, E. irresidua, E. exiqua, E. coecicola, and E. piriformis) were observed in all farms. The most pathogenic species--E. intestinalis was found both in farm A and B, whereas E. flavescens and E. stiedae--only in the former. The level of infection was high, especially in young rabbits, with the prevalence of 94.9 to 100% and mean intensity from 11,161 to 28,871 oocysts per 1 g of faeces (OPG) in a particular warren. The highest intensity of infection was observed in May, when the mean output increased to 29,454, 56,952, and 23,815 OPG in warrens A, B, and C, respectively. A nematode, Passalurus ambiguus, was detected in all searched farms, with the prevalence from 14.1% to 27.5%, depending on a farm. The species was more often seen in the first part of year. The other helminths (Trichostrongylus retortaeformis, Graphidium strigosum, and Trichuris leporis) were found only in

  4. Is there a difference between hare syphilis and rabbit syphilis? Cross infection experiments between rabbits and hares.

    Science.gov (United States)

    Lumeij, Johannes T; Mikalová, Lenka; Smajs, David

    2013-05-31

    Cross infection of rabbits and hares with Treponema paraluiscuniculi from rabbits and the related microorganism from hares, which was provisionally named "Treponema paraluisleporis", revealed that T. paraluiscuniculi affects rabbits clinically, but only causes seroconversion in hares without causing clinical disease, while "T. paraluisleporis" induces disease in both rabbits and hares. The 16S rRNA gene of "T. paraluisleporis" was sequenced (GenBank acc. no. JX899416) and compared to the sequence of T. paraluiscuniculi strain Cuniculi A. A phylogenetic tree based on the sequence alignment of 2002 bp taken from several treponemal strains was constructed. Both "T. paraluisleporis" and T. paraluiscuniculi are clustered together indicating their common origin. The close phylogenetic relatedness of both representatives supports the conclusion that subspecies or ecovar status should be given to these strains rather than species status. A more appropriate species name might be Treponema paraluisleporidarum. The genitive refers to the nominative Leporidae (family of rabbits and hares). The naturally occurring strain in rabbits would than be T. paraluisleporidarum ecovar Cuniculus and the strain from hares T. paraluisleporidarum ecovar Lepus. Since the former seems to have fewer physiological hosts, ecovar Lepus may represent an evolutionary ancestor of ecovar Cuniculus.

  5. Prevention and treatment of Encephalitozoon cuniculi infection in rabbits with fenbendazole

    OpenAIRE

    C. Suter; Müller-Doblies, U. U.; Hatt, Jean-Michel; Deplazes, Peter

    2001-01-01

    The efficacy of fenbendazole for preventing an experimental infection of Encephalitozoon cuniculi and for eliminating the spores from the central nervous system of naturally infected rabbits was investigated. Fenbendazole (20 mg/kg bodyweight daily) was administered from seven days before until two or 21 days after rabbits had been infected orally with 10(6) spores of E. cuniculi. Both regimens were effective in preventing the establishment of the parasites, as demonstrated by negative parasi...

  6. Seroprevalence and Risk Factors of Chlamydia Infection in Domestic Rabbits (Oryctolagus cuniculus in China

    Directory of Open Access Journals (Sweden)

    Xiaoting Ni

    2015-01-01

    Full Text Available Chlamydia spp. are obligate intracellular bacteria distributed all over the world, known to cause various forms of diseases in animals and humans. In the present study, a serological survey was conducted to detect the seroprevalence and risk factors associated with rabbit chlamydiosis in northeast China, including Liaoning province, Jilin province, Heilongjiang province, and Inner Mongolia Autonomous Region. Antibodies to Chlamydia were determined by indirect hemagglutination assay (IHA. The overall seroprevalence was estimated at 17.88% in total of 800 blood samples. The Chlamydia seroprevalence varied in domestic rabbits from different factors, and genders of domestic rabbits were considered as major risk factors associated with Chlamydia infection. Our study revealed a widespread and high prevalence of Chlamydia infection in domestic rabbits in northeast China, with higher exposure risk in female domestic rabbits. These findings suggested the potential importance of domestic rabbits in the transmission of zoonotic Chlamydia infection, and thus Chlamydia should be taken into consideration in diagnosing rabbit diseases. To our knowledge, there is no report of Chlamydia infection in domestic rabbits in China and the results extend the host range for Chlamydia, which has important implications for public health and the local economy.

  7. Seroprevalence and Risk Factors of Chlamydia Infection in Domestic Rabbits (Oryctolagus cuniculus) in China.

    Science.gov (United States)

    Ni, Xiaoting; Qin, Siyuan; Lou, Zhilong; Ning, Hongrui; Sun, Xiaolin

    2015-01-01

    Chlamydia spp. are obligate intracellular bacteria distributed all over the world, known to cause various forms of diseases in animals and humans. In the present study, a serological survey was conducted to detect the seroprevalence and risk factors associated with rabbit chlamydiosis in northeast China, including Liaoning province, Jilin province, Heilongjiang province, and Inner Mongolia Autonomous Region. Antibodies to Chlamydia were determined by indirect hemagglutination assay (IHA). The overall seroprevalence was estimated at 17.88% in total of 800 blood samples. The Chlamydia seroprevalence varied in domestic rabbits from different factors, and genders of domestic rabbits were considered as major risk factors associated with Chlamydia infection. Our study revealed a widespread and high prevalence of Chlamydia infection in domestic rabbits in northeast China, with higher exposure risk in female domestic rabbits. These findings suggested the potential importance of domestic rabbits in the transmission of zoonotic Chlamydia infection, and thus Chlamydia should be taken into consideration in diagnosing rabbit diseases. To our knowledge, there is no report of Chlamydia infection in domestic rabbits in China and the results extend the host range for Chlamydia, which has important implications for public health and the local economy.

  8. Diagnosis and Etiology of Secondary Infection of Escherichia coli with Rabbit Coccidiosis

    Institute of Scientific and Technical Information of China (English)

    Luo; Shimin; Hu; Hui; Huang; Guangzhong; Su; Wuzhen; Yao; Guomin; Huang; Minsheng; Hu; Yan

    2014-01-01

    In November 2012,diarrhea-predominant disease broke out in a large rabbit farm in Huaihua City,leading to mortality rate of 5. 3%. Through survey of incidence,combined with clinical symptoms,pathological changes and laboratory diagnosis,the disease was confirmed as secondary infection of E. coli with rabbit coccidiosis,and it had been controlled after drug treatment.

  9. A Probability Analysis of Historical Pregnancy and Fetal Data from Dutch Belted and New Zealand White Rabbit Strains from Embryo-Fetal Development Studies.

    Science.gov (United States)

    Posobiec, Lorraine M; Cox, Estella M; Solomon, Howard M; Lewis, Elise M; Wang, Kai-fen; Stanislaus, Dinesh

    2016-04-01

    Embryo-fetal development (EFD) studies, typically in pregnant rats and rabbits, are conducted prior to enrolling females of reproductive age in clinical trials. Common rabbit strains used are the New Zealand White (NZW) and Dutch Belted (DB). As fetal abnormalities can occur in all groups, including controls, Historical Control Data (HCD) is compiled using data from control groups of EFD studies, and is used along with each study's concurrent control group to help determine whether fetal abnormalities are caused by the test article or are part of background incidences. A probability analysis was conducted on 2014 HCD collected at Charles River Inc., Horsham PA on Covance NZW, Covance DB, and Charles River (CR) NZW rabbits. The analysis was designed to determine the probability of 2 or 3 out of a group of 22 does aborting their litter or of having a fetal abnormality by chance. Results demonstrate that pregnancy parameters and fetal observations differ not only between strains, but between sources of rabbits of the same strain. As a result the probability of these observations occurring by chance in two or three litters was drastically different. Although no one single strain is perfect, this analysis highlights the need to appreciate the inherent differences in pregnancy and fetal abnormalities between strains, and points out that an apparent isolated increased incidence of an observation in one strain will not necessarily be test-article related in another strain. A robust HCD is critical for interpretation of EFD rabbit studies, regardless of the rabbit strain used.

  10. Quantification of HTLV-I proviral load in experimentally infected rabbits

    Directory of Open Access Journals (Sweden)

    Kindt Thomas J

    2005-05-01

    Full Text Available Abstract Background Levels of proviral load in HTLV-1 infected patients correlate with clinical outcome and are reasonably prognostic. Adaptation of proviral load measurement techniques is examined here for use in an experimental rabbit model of HTLV-1 infection. Initial efforts sought to correlate proviral load with route and dose of inoculation and with clinical outcome in this model. These methods contribute to our continuing goal of using the model to test treatments that alleviate virus infection. Results A real-time PCR assay was used to measure proviral load in blood and tissue samples from a series of rabbits infected using HTLV-1 inocula prepared as either cell-free virus particles, infected cells or blood, or by naked DNA injection. Proviral loads from asymptomatically infected rabbits showed levels corresponding to those reported for human patients with clinically silent HTLV-1 infections. Proviral load was comparably increased in 50% of experimentally infected rabbits that developed either spontaneous benign or malignant tumors while infected. Similarly elevated provirus was found in organs of rabbits with experimentally induced acute leukemia/lymphoma-like disease. Levels of provirus in organs taken at necropsy varied widely suggesting that reservoirs of infections exist in non-lymphoid organs not traditionally thought to be targets for HTLV-1. Conclusion Proviral load measurement is a valuable enhancement to the rabbit model for HTLV-1 infection providing a metric to monitor clinical status of the infected animals as well as a means for the testing of treatment to combat infection. In some cases proviral load in blood did not reflect organ proviral levels, revealing a limitation of this method for monitoring health status of HTLV-1 infected individuals.

  11. Infection in rabbits with the Lyme disease spirochete.

    Science.gov (United States)

    Kornblatt, A. N.; Steere, A. C.; Brownstein, D. G.

    1984-01-01

    Of 33 rabbits inoculated with Lyme disease spirochetes, two developed erythema chronicum migrans at the site of inoculation. Spirochetes were seen in skin biopsies of one of the lesions with immunoperoxidase and Warthin-Starry stains. Spirochetes were also recovered from the blood of two additional rabbits two weeks post-inoculation. These findings are characteristic of early Lyme disease in humans. PMID:6393613

  12. A Seroepidemiological Study of Serogroup A Meningococcal Infection in the African Meningitis Belt.

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    Olivier Manigart

    Full Text Available The pattern of epidemic meningococcal disease in the African meningitis belt may be influenced by the background level of population immunity but this has been measured infrequently. A standardised enzyme-linked immunosorbent assay (ELISA for measuring meningococcal serogroup A IgG antibodies was established at five centres within the meningitis belt. Antibody concentrations were then measured in 3930 individuals stratified by age and residence from six countries. Seroprevalence by age was used in a catalytic model to determine the force of infection. Meningococcal serogroup A IgG antibody concentrations were high in each country but showed heterogeneity across the meningitis belt. The geometric mean concentration (GMC was highest in Ghana (9.09 μg/mL [95% CI 8.29, 9.97] and lowest in Ethiopia (1.43 μg/mL [95% CI 1.31, 1.57] on the margins of the belt. The force of infection was lowest in Ethiopia (λ = 0.028. Variables associated with a concentration above the putative protective level of 2 μg/mL were age, urban residence and a history of recent vaccination with a meningococcal vaccine. Prior to vaccination with the serogroup A meningococcal conjugate vaccine, meningococcal serogroup A IgG antibody concentrations were high across the African meningitis belt and yet the region remained susceptible to epidemics.

  13. Efficacy of garlic extract on hepatic coccidiosis in infected rabbits (Oryctolagus cuniculus): histological and biochemical studies.

    Science.gov (United States)

    Toulah, Fawzia H; Al-Rawi, Maisaa M

    2007-12-01

    The rabbits were divided into three groups, of 12 rabbits each. G1 was the (non-infected non-treated) as control, G2 was the (infected-non treated), and G3 was the (infected and treated) rabbits. Each rabbit in the infected groups were given (10(3)) sporuleted oocysts of Eimeria stiedae per rabbit after forty five days exactly. Faecal sample of rabbits from each group were examined each day post infection till oocysts appeared in faeces. The treatment was given by using suitable dosage of garlic according to body weight. After 15, 21, 28, & 35 days post-treatment faecal oocysts were output. Biochemical parameters as serum liver function (ALT, AST, GGT & ALP) that denoted the he-patic cells injury. The results showed a significant differences in the mean values of oocysts shedding and their mean number in bile ducts between Gs 2 &3 from the 15th day post infection (PI) (mean +/-SD:40.33 +/- 16.72 & 25.17 +/- .56 respectively) till the experimental end on the 35th day (55.75 +/- 19.79 & 0.94 +/- 1.43 respectively). The histopathological alterations were in liver of G2 at the experimental end. Coccidiosis in G2 induced histopathological alterations in liver tissue, marked cytoplasmic vacuolations in hepatocytes with clear signs of karyolysis, and dilatation of sinusoids with increase in Kupffer cells. Leukocytic infiltration around congested blood vessels was noticed. Efficacy of garlic on E. stiedae in infected Gs was resident. The liver of G3 regained almost normal appearance compared to control.

  14. Mycobacterium avium subspecies paratuberculosis infection modifies gut microbiota under different dietary conditions in a rabbit model

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    Rakel eArrazuria

    2016-03-01

    Full Text Available Mycobacterium avium subspecies paratuberculosis (MAP the causative agent of paratuberculosis, produces a chronic granulomatous inflammation of the gastrointestinal tract of ruminants. It has been recently suggested that MAP infection may be associated with dysbiosis of intestinal microbiota in ruminants. Since diet is one of the key factors affecting the balance of microbial populations in the digestive tract, we intended to evaluate the effect of MAP infection in a rabbit model fed a regular or high fiber diet during challenge. The composition of microbiota of the cecal content and the sacculus rotundus was studied in 20 New Zealand white female rabbits. The extracted DNA was subjected to paired-end Illumina sequencing of the V3-V4 hypervariable region of the 16S rRNA gene for microbiota analysis. Microbial richness (Chao1 in the cecal content was significantly increased by MAP infection in regular diet rabbits (p = 0.0043 and marginally increased (p = 0.0503 in the high fiber group. Analysis of beta-diversity showed that MAP infection produces deeper changes in the microbiota of sacculus rotundus than in the cecal content. A lower abundance of Proteobacteria in the cecal content of infected animals fed the high fiber diet and also lower abundance of Bacteroidetes in the sacculus rotundus of infected animals fed the regular diet were observed. Based on OPLS-DA analysis, we observed that some bacteria repeatedly appear positively associated with infection in different samples under different diets (families Dehalobacteriaceae, Coriobacteriaceae and Mogibacteriaceae; genus Anaerofustis. The same phenomenon was observed with some of the bacteria negatively associated with MAP infection (genera Anaerostipes and Coprobacillus. However, other groups of bacteria (Enterobacteriaceae family and ML615J-28 order were positively associated with infection in some circumstances and negatively associated with infection in others.Data demonstrate that MAP

  15. Eimeria stiedae: experimental infection in rabbits and the effect of treatment with toltrazuril and ivermectin.

    Science.gov (United States)

    Cam, Yücel; Atasever, Ayhan; Eraslan, Gökhan; Kibar, Murat; Atalay, Oznur; Beyaz, Latife; Inci, Abdullah; Liman, Bilal Cem

    2008-05-01

    The aim of this study was to investigate the clinical, haematological, biochemical, lipid peroxidation, ultrasonographic and pathologic findings in hepatic coccidiosis induced by Eimeria stiedae in rabbits, and also to compare the treatment effects of both toltrazuril and ivermectin separately and in combination. In this study, 56 rabbits were divided into eight groups. The first group was designated as healthy control group. Rabbits were infected with 40.000 sporulated oocysts of E. stiedae. Groups 2, 3, 4, 5, 6, 7 and 8 were allocated as the infected control group, infected+toltrazuril-treated group, infected+ivermectin-treated group, infected+toltrazuril+ivermectin-treated group, non-infected+toltrazuril-treated group, non-infected+ivermectin-treated group, non-infected+toltrazuril+ivermectin-treated group, respectively. Haematocrit, Haemoglobin and MCV values as well as percentage of lymphocyte decreased in Groups 2 and 4 whereas leucocyte counts and percentage of granulocyte leucocyte increased. Serum GGT, ALT and AST activities increased but albumin value decreased. Plasma MDA concentrations increased whereas erythrocyte CAT, GSH-Px, and SOD activities decreased. Mean oocyst numbers in per gram faeces (epg values) increased in both groups during the study. Ultrasonographic examination revealed that the liver was enlarged and had hyperechogenic parenchyma. Bile ducts were dilated and hyperechogenic and the gall bladder was dilated. The livers of these animals were enlarged and typical macroscopic and microscopic findings of coccidiosis were present. Treatment with toltrazuril and toltrazuril+ivermectin combination were highly effective in reducing faecal oocyst output in infected rabbits. Haematological, biochemical and lipid peroxidation parameters and, ultrasonographic findings of the liver were close to control values for Groups 3 and 5. Necropsy of these animals showed no visible lesions related to hepatic coccidiosis although a few oocysts were detected

  16. A new rabbit model of implant-related biofilm infection: development and evaluation

    Science.gov (United States)

    Chu, Cheng-Bing; Zeng, Hong; Shen, Ding-Xia; Wang, Hui; Wang, Ji-Fang; Cui, Fu-Zhai

    2016-03-01

    This study is to establish a rabbit model for human prosthetic joint infection and biofilm formation. Thirty-two healthy adult rabbits were randomly divided into four groups and implanted with stainless steel screws and ultra-high molecular weight polyethylene (UHMWPE) washers in the non-articular surface of the femoral lateral condyle of the right hind knees. The rabbit knee joints were inoculated with 1 mL saline containing 0, 102, 103, 104 CFU of Staphylococcus epidermidis ( S. epidermidis) isolated from the patient with total knee arthroplasty (TKA) infection, respectively. On the 14th postoperative day, the UHMWPE washers from the optimal 103 CFU group were further examined. The SEM examination showed a typical biofilm construction that circular S. epidermidis were embedded in a mucous-like matrix. In addition, the LCSM examination showed that the biofilm consisted of the polysaccharide stained bright green fluorescence and S. epidermidis radiating red fluorescence. Thus, we successfully create a rabbit model for prosthetic joint infection and biofilm formation, which should be valuable for biofilm studies.

  17. Protection of rabbits and immunodeficient mice against lethal poxvirus infections by human monoclonal antibodies.

    Directory of Open Access Journals (Sweden)

    Lindsay Crickard

    Full Text Available Smallpox (variola virus is a bioweapon concern. Monkeypox is a growing zoonotic poxvirus threat. These problems have resulted in extensive efforts to develop potential therapeutics that can prevent or treat potentially lethal poxvirus infections in humans. Monoclonal antibodies (mAbs against smallpox are a conservative approach to this problem, as the licensed human smallpox vaccine (vaccinia virus, VACV primarily works on the basis of protective antibody responses against smallpox. Fully human mAbs (hmAbs against vaccinia H3 (H3L and B5 (B5R, targeting both the mature virion (MV and extracellular enveloped virion (EV forms, have been developed as potential therapeutics for use in humans. Post-exposure prophylaxis was assessed in both murine and rabbit animal models. Therapeutic efficacy of the mAbs was assessed in three good laboratory practices (GLP studies examining severe combined immunodeficiency mice (SCID given a lethal VACV infection. Pre-exposure combination hmAb therapy provided significantly better protection against disease and death than either single hmAb or vaccinia immune globulin (VIG. Post-exposure combination mAb therapy provided significant protection against disease and death, and appeared to fully cure the VACV infection in ≥50% of SCID mice. Therapeutic efficacy was then assessed in two rabbit studies examining post-exposure hmAb prophylaxis against rabbitpox (RPXV. In the first study, rabbits were infected with RPVX and then provided hmAbs at 48 hrs post-infection, or 1 hr and 72 hrs post-infection. Rabbits in both groups receiving hmAbs were 100% protected from death. In the second rabbitpox study, 100% of animal treated with combination hmAb therapy and 100% of animals treated with anti-B5 hmAb were protected. These findings suggest that combination hmAb treatment may be effective at controlling smallpox disease in immunocompetent or immunodeficient humans.

  18. Efficacy of gallium maltolate against Lawsonia intracellularis infection in a rabbit model.

    Science.gov (United States)

    Sampieri, F; Allen, A L; Alcorn, J; Clark, C R; Vannucci, F A; Pusterla, N; Mapes, S M; Ball, K R; Dowling, P M; Thompson, J; Bernstein, L R; Gebhart, C J; Hamilton, D L

    2014-12-01

    Antimicrobial efficacy against Lawsonia intracellularis is difficult to evaluate in vitro, thus, the effects of gallium maltolate's (GaM) were investigated in a rabbit model for equine proliferative enteropathy (EPE). Juvenile (5-6-week-old) does were infected with 3.0 × 10(8) L. intracellularis/rabbit and allocated into three groups (n = 8). One week postinfection, one group was treated with GaM, 50 mg/kg; one, with doxycycline, 5 mg/kg; and one with a sham-treatment (control). Feces and blood were collected daily and weekly, respectively, to verify presence of L. intracellularis fecal shedding using qPCR, and seroconversion using immunoperoxidase monolayer assay. Rabbits were sacrificed after 1 week of treatment to collect intestinal tissues focusing on EPE-affected sections. Intestinal lesions were confirmed via immunohistochemistry. No difference was noted between treatments regarding EPE-lesions in jejunum (P = 0.51), ileum (P = 0.74), and cecum (P = 0.35), or in L. intracellularis fecal shedding (P = 0.64). GaM and doxycycline appear to have similar efficacy against EPE in infected rabbits.

  19. Identification of toxocara canis antigens by Western blot in experimentally infected rabbits

    Directory of Open Access Journals (Sweden)

    MORALES Olga Lucía

    2002-01-01

    Full Text Available Toxocariasis is a frequent helminthiasis that can cause visceral and ocular damage in humans specially in children. The identification of specific antigens of Toxocara canis is important in order to develop better diagnostic techniques. Ten rabbits were infected orally with a dose of 5000 Toxocara canis embryonated eggs. Rabbits were bled periodically and an ELISA assay was performed to determine levels of specific Toxocara IgG antibodies. ELISA detected antibodies at day 15 after infection. Western blot (WB assay was performed using excretory/secretory antigens (E/S of T. canis second stage larvae. Different antigen concentrations were evaluated: 150, 200, 250 and 300 µg/mL. The concentration of 250 µg/mL was retained for analysis. Rabbit sera were diluted 1:100. Secondary antibody was used at a dilution of 1:1000. Results of WB indicated that in the first month after infection specific antibodies against the 200 KDa, 116 KDa, 92 KDa and 35 KDa antigens were detected; antibodies against the 92 KDa, 80 KDa, 66 KDa, 45 KDa, 31 KDa and 28 KDa antigens appeared later. All positive sera in the ELISA test were also positive in WB. Two antigen bands, 92 KDa and 35 KDa, were identified since the beginning and throughout the course of infection. These antigens merit further evaluation as candidates for use in diagnosis.

  20. Toxoplasma gondii infection in the mountain hare (Lepus timidus) and domestic rabbit (Oryctolagus cuniculus). II. Early immune reactions

    DEFF Research Database (Denmark)

    Gustafsson, K.; Wattrang, E.; Fossum, C.;

    1997-01-01

    of the acute phase reactant haptoglobin were raised in hares but not in rabbits one week post-infection (pi), probably reflecting the severe tissue damage present. No difference in the early humoral immune response of hares and rabbits was found, both species producing IgM and IgG antibodies to T. gondii one...... week pi. Lymphocyte stimulation tests performed before and one week after inoculation showed a high proliferative response to the parasite in blood cell cultures from rabbits but not hares. The fatal outcome of T. gondii infection in the hares is probably due, at least in part, to the lack of cellular...

  1. Main pathologies associated with "Staphylococcus aureus" infections in rabbits: a review

    OpenAIRE

    Haesebrouck, F.; Corpa, J.M.; Hermans, K.

    2009-01-01

    Staphylococcus aureus is a versatile opportunistic pathogen that causes a wide spectrum of pathologies. In rabbits, this bacterium infects dermal lesions causing suppurative dermatitis, and invades subcutaneous tissues, causing different well-known disease conditions such as mastitis, abscesses (subcutaneously or affecting internal organs) and pododermatitis. However, the lesions associated with S. aureus have rarely been described in detail in the literature. The aim of this paper is to upda...

  2. A Rabbit Model of Acanthamoeba Keratitis That Better Reflects the Natural Human Infection.

    Science.gov (United States)

    Feng, Xianmin; Zheng, Wenyu; Wang, Yuehua; Zhao, Donghai; Jiang, Xiaoming; Lv, Shijie

    2015-08-01

    Acanthamoeba species are ubiquitous, free-living protozoa that can invade the cornea and result in Acanthamoeba keratitis (AK), a painful progressive sight-threatening corneal disease. Disease progression in current animal models is too rapid to mimic AK in humans accurately. This study provides a novel method for establishing AK in rabbits and compared it with the conventional method with regard to pathogenesis and immune response in humans. The New Zealand white rabbits were randomly divided into two experimental groups (Groups A and B). Rabbits in the Group A (n = 14) received intrastromal injections of 1 × 10(4) /100 µL Acanthamoeba healyi trophozoites (conventional AK model). The Group B animals (n = 14) received microinjections of 1 × 10(4) /10 µL A. healyi trophozoites between the corneal epithelium and Bowman's layer, anterior to the corneal stroma (novel AK model). In addition, two rabbits were left untreated as normal controls. AK in the treated rabbits was evaluated clinically, histopathologically, and immunologically for 35 days. AK was successfully established in both the conventional and novel model groups. Compared with the Group A, AK in the Group B displayed an efficient immune response with less severe pathology. Moreover, the self-limiting but chronic nature of the infection in the Group B was strikingly similar to that of AK in humans. The novel animal model for AK described here more closely simulates the pathogenesis and immune response of Acanthamoeba corneal infection in humans than the animal models currently in use.

  3. Expression of IL-1β, IL-2, IL-10, TNF-β and GM-CSF in peripheral blood leukocytes of rabbits experimentally infected with rabbit haemorrhagic disease virus.

    Science.gov (United States)

    Trzeciak-Ryczek, Alicja; Tokarz-Deptuła, Beata; Deptuła, Wiesław

    2016-04-15

    Rabbit haemorrhagic disease (RHD) is a highly morbid and mortal viral infection of European rabbits. This disease is one of the main causes of death in wild rabbits, and results in large economic losses in farms of rabbits worldwide. Although the first outbreak of this disease was noted in 1984, the pathogenesis of RHD and mechanisms of RHDV (rabbit haemorrhagic disease virus) pathogenecity have still not been fully elucidated. Recent studies indicate a role of the immune response, especially peripheral blood leukocytes (PBL), in the pathogenesis of this disease. Thus, in the present study we investigated the expression of IL-1β, IL-2, IL-10, TNF-β and GM-CSF genes in PBL of RHDV-infected rabbits. We also compared the expression of genes encoding these cytokines in rabbits with different course of RHDV infection (in animals that died 36h postinfection or survived until 60th h after infection). The study revealed that three (IL-10, TNF-β and GM-CSF) out of five investigated genes encoding cytokines showed increased expression in PBL of RHDV-infected rabbits, and the level of expression depended on the course of RHD. The results indicate the potential role of these cytokines in RHDV infection and their influence on the survival time of infected rabbits.

  4. Nerve growth factor antibody stimulates reactivation of ocular herpes simplex virus type 1 in latently infected rabbits.

    Science.gov (United States)

    Hill, J M; Garza, H H; Helmy, M F; Cook, S D; Osborne, P A; Johnson, E M; Thompson, H W; Green, L C; O'Callaghan, R J; Gebhardt, B M

    1997-06-01

    Anti-nerve growth factor (anti-NGF) antibody has been shown to induce reactivation of latent herpes simplex virus type 1 (HSV-1) in vitro. We found that systemically administered anti-NGF induces ocular shedding of HSV-1 in vivo in rabbits harboring latent virus. Rabbits in which HSV-1 latency had been established were given intravenous injections of goat anti-NGF serum daily for 10 days beginning 42 days after primary viral infection. Tears were assayed for virus for 12 days beginning on the day of the first injection. All eight rabbits given high titer anti-NGF had infectious virus in their tears at least once during the 12-day period. Fifteen of 16 eyes were positive and the average duration of viral shedding for these eyes was 4.0 days. Latently infected rabbits receiving daily injections of nonimmune goat serum or saline for 10 consecutive days were controls. Only six of the 16 (38%) eyes from rabbits receiving nonimmune goat serum shed virus. Only one of 12 eyes from untreated rabbits shed virus. Sera from control rabbits had no detectable anti-NGF activity; titers in anti-NGF-treated rabbits ranged between 1:1000 and 1:10,000. NGF deprivation may act as a neuronal stressor and may share a common second messenger pathway with heat- or cold-stress induced reactivation of latent HSV-1.

  5. The complete mitochondrial genomes of five Eimeria species infecting domestic rabbits.

    Science.gov (United States)

    Liu, Guo-Hua; Tian, Si-Qin; Cui, Ping; Fang, Su-Fang; Wang, Chun-Ren; Zhu, Xing-Quan

    2015-12-01

    Rabbit coccidiosis caused by members of the genus Eimeria can cause enormous economic impact worldwide, but the genetics, epidemiology and biology of these parasites remain poorly understood. In the present study, we sequenced and annotated the complete mitochondrial (mt) genomes of five Eimeria species that commonly infect the domestic rabbits. The complete mt genomes of Eimeria intestinalis, Eimeria flavescens, Eimeria media, Eimeria vejdovskyi and Eimeria irresidua were 6261bp, 6258bp, 6168bp, 6254bp, 6259bp in length, respectively. All of the mt genomes consist of 3 genes for proteins (cytb, cox1, and cox3), 14 gene fragments for the large subunit (LSU) rRNA and 11 gene fragments for the small subunit (SSU) rRNA, but no transfer RNA (tRNA) genes. The gene order of the mt genomes is similar to that of Plasmodium, but distinct from Haemosporida and Theileria. Phylogenetic analyses based on full nucleotide sequences using Bayesian analysis revealed that the monophyly of the Eimeria of rabbits was strongly statistically supported with a Bayesian posterior probabilities. These data provide novel mtDNA markers for studying the population genetics and molecular epidemiology of the Eimeria species, and should have implications for the molecular diagnosis, prevention and control of coccidiosis in rabbits.

  6. Surveillance for an outbreak of Encephalitozoon cuniculi infection in rabbits housed at a zoo and biosecurity countermeasures.

    Science.gov (United States)

    Fukui, Daisuke; Bando, Gen; Furuya, Koji; Yamaguchi, Masanori; Nakaoka, Yuji; Kosuge, Masao; Murata, Koichi

    2013-01-31

    An outbreak of encephalitozoonosis occurred in a rabbit colony at a zoo in Japan. Throughout the two years after the onset, all 42 rabbits were investigated clinically, pathologically and serologically for prevention and control of the disease. Eleven rabbits (11/42, 26.2%) showed clinical symptoms. Of 38 rabbits examined to detect specific antibodies against Encephalitozoon cuniculi, 71.1% (n=27) were found seropositive; 20 out of 30 clinically healthy rabbits (except for 8 clinical cases) were seropositive. The infection rate was 76.2% (32/42), including 5 pathologically diagnosed cases. The results of serological survey revealed that asymptomatic infection was widespread, even among clinically healthy rabbits. However, encephalitozoonosis was not found by pathological examination in any other species of animals kept in the same area within the zoo. Isolation and elimination of the rabbits with suspected infection based on the results of serological examination were carried out immediately; however, encephalitozoonosis continued to occur sporadically. Therefore, all the remaining rabbits were finally slaughtered. Then, the facility was closed, and all the equipment was disinfected. After a two-month interval, founder rabbits were introduced from encephalitozoonosis-free rabbitries for new colony formation. Since then, encephalitozoonosis has not been seen in any animals at the zoo. In this study, biosecurity countermeasures including staff education, epidemiological surveillance and application of an "all-out and all-in" system for rabbit colony establishment based on serological examination were successfully accomplished with regard to animal hygiene and public health for the eradication of E. cuniculi.

  7. Influence of Pasteurella multocida infection on the pharmacokinetic behavior of marbofloxacin after intravenous and intramuscular administrations in rabbits.

    Science.gov (United States)

    Abo-el-Sooud, K; Goudah, A

    2010-02-01

    The pharmacokinetic behavior of marbofloxacin was studied in healthy (n = 12) and Pasteurella multocida infected rabbits (n = 12) after single intravenous (i.v.) and intramuscular (i.m.) administrations. Six rabbits in each group (control and diseased) were given a single dose of 2 mg/kg body weight (bw) of marbofloxacin intravenously. The other six rabbits in each group were given the same dose of the drug intramuscularly. The concentration of marbofloxacin in plasma was determined using high-performance liquid chromatography. The plasma concentrations were higher in diseased rabbits than in healthy rabbits following both routes of injections. Following i.v. administration, the values of the elimination half-life (t(1/2beta)), and area under the curve were significantly higher, whereas total body clearance was significantly lower in diseased rabbits. After i.m. administration, the elimination half-life (t(1/2el)), mean residence time, and maximum plasma concentration (C(max)) were higher in diseased rabbits (5.33 h, 7.35 h and 2.24 microg/mL) than in healthy rabbits (4.33 h, 6.81 h and 1.81 microg/mL, respectively). Marbofloxacin was bound to the extent of 26 +/- 1.3% and 23 +/- 1.6% to plasma protein of healthy and diseased rabbits, respectively. The C(max)/MIC (minimum inhibitory concentration) and AUC/MIC ratios were significantly higher in diseased rabbits (28 and 189 h) than in healthy rabbits (23 and 157 h), indicating the favorable pharmacodynamic characteristics of the drug in diseased rabbits.

  8. Cholinesterase as inflammatory markers in a experimental infection by Trypanosoma evansi in rabbits

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    Márcio M. Costa

    2012-12-01

    Full Text Available The aim of this study is to evaluate the role of cholinesterases as an inflammatory marker in acute and chronic infection by Trypanosoma evansi in rabbits experimentally infected. Twelve adult female New Zealand rabbits were used and divided into two groups with 6 animals each: control group (rabbits 1-6 and infected group (rabbits 7-12. Infected group received intraperitoneally 0.5 mL of blood from a rat containing 108 parasites per animal. Blood samples used for cholinesterases evaluation were collected on days 0, 2, 7, 12, 27, 42, 57, 87, 102 and 118 days post-inoculation (PI. Increased activity (P0.05 was observed in the encephalic structures. The increased activities of AChE and BChE probably have a pro-inflammatory purpose, attempting to reduce the concentration of acetylcholine, a neurotransmitter which has an anti-inflammatory property. Therefore, cholinesterase may be inflammatory markers in infection with T. evansi in rabbits.O objetivo do presente estudo é avaliar o papel das colinesterases como marcadores inflamatórios nas fases aguda e crônica da infecção por T. evansi em coelhos infectados experimentalmente. Foram utilizados 12 coelhos adultos, fêmeas, da raça Nova Zelândia, divididos em dois grupos: um grupo controle, com seis animais (coelhos 1-6, e um grupo infectado, com seis animais (coelhos 7-12. Os animais pertencentes ao grupo infectados receberam, pela via intraperitoneal, 0,5 mL de sangue de rato contendo 108 tripanossomas por animal. Amostras do sangue utilizado para avaliação das colinesterases foram coletadas nos dias 0, 2, 7, 12, 27, 42, 57, 87, 102 e 118 pós-inoculação (PI. Aumento (P0,05 foi observada nas estruturas encefálicas. O aumento de atividade da AChE e BChE provavelmente tenha finalidade pró-inflamatória, a fim de reduzir as concentrações de acetilcolina, neurotransmissor que apresenta propriedade anti-inflamatória. Portanto, as colinesterases podem ser marcadores inflamatórios na infec

  9. Molecular diagnosis of Eimeria stiedae in hepatic tissue of experimentally infected rabbits.

    Science.gov (United States)

    Hassan, Khaled M; Arafa, Waleed M; Mousa, Waheed M; Shokier, Khaled A M; Shany, Salama A; Aboelhadid, Shawky M

    2016-10-01

    The early detection of Eimeria stiedae in the hepatic tissue of experimentally infected rabbits was investigated using molecular assay. Forty 6-week-old male New Zealand rabbits were divided into two groups. Group A (30 animals) was infected with 2.5 × 10(4) sporulated oocysts of E. stiedae per animal on Day 0 and Group B (10 animals) was used as the uninfected controls. Three animals from Group A and one from Group B were sacrificed at 0, 3, 6, 9, 12, 15, 18, 21, 24 and 27 days post infection (PI). Gross and microscopic post-mortem findings were recorded. Polymerase chain reaction (PCR) of the E. stiedae internal transcribed spacer 1 genomic region was conducted on blood, liver tissue, and feces from the Group A experimentally infected animals. Macroscopically, the liver showed irregular yellowish white nodules pathognomonic to E. stiedae infection beginning on Day 15 PI. Hepatomegaly and ascites were obvious from Day 21-24 PI. The presence of different E. stiedae schizonts and gametocytes in the histopathological sections of the biliary epithelium were evident on Day 15 PI. The E. stiedae PCR was first positive in liver tissues on Day 12 and in fecal samples on Day 18 PI, but the blood samples were negative. In conclusion, the PCR can be used for early diagnosis and control of E. stiedae schizonts before shedding of the oocysts in feces.

  10. Experimental West Nile Virus Infection in Rabbits: An Alternative Model for Studying Induction of Disease and Virus Control.

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    Suen, Willy W; Uddin, Muhammad J; Wang, Wenqi; Brown, Vienna; Adney, Danielle R; Broad, Nicole; Prow, Natalie A; Bowen, Richard A; Hall, Roy A; Bielefeldt-Ohmann, Helle

    2015-07-14

    The economic impact of non-lethal human and equine West Nile virus (WNV) disease is substantial, since it is the most common presentation of the infection. Experimental infection with virulent WNV strains in the mouse and hamster models frequently results in severe neural infection and moderate to high mortality, both of which are not representative features of most human and equine infections. We have established a rabbit model for investigating pathogenesis and immune response of non-lethal WNV infection. Two species of rabbits, New Zealand White (Oryctolagus cuniculus) and North American cottontail (Sylvilagus sp.), were experimentally infected with virulent WNV and Murray Valley encephalitis virus strains. Infected rabbits exhibited a consistently resistant phenotype, with evidence of low viremia, minimal-absent neural infection, mild-moderate neuropathology, and the lack of mortality, even though productive virus replication occurred in the draining lymph node. The kinetics of anti-WNV neutralizing antibody response was comparable to that commonly seen in infected horses and humans. This may be explained by the early IFNα/β and/or γ response evident in the draining popliteal lymph node. Given this similarity to the human and equine disease, immunocompetent rabbits are, therefore, a valuable animal model for investigating various aspects of non-lethal WNV infections.

  11. Experimental West Nile Virus Infection in Rabbits: An Alternative Model for Studying Induction of Disease and Virus Control

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    Willy W. Suen

    2015-07-01

    Full Text Available The economic impact of non-lethal human and equine West Nile virus (WNV disease is substantial, since it is the most common presentation of the infection. Experimental infection with virulent WNV strains in the mouse and hamster models frequently results in severe neural infection and moderate to high mortality, both of which are not representative features of most human and equine infections. We have established a rabbit model for investigating pathogenesis and immune response of non-lethal WNV infection. Two species of rabbits, New Zealand White (Oryctolagus cuniculus and North American cottontail (Sylvilagus sp., were experimentally infected with virulent WNV and Murray Valley encephalitis virus strains. Infected rabbits exhibited a consistently resistant phenotype, with evidence of low viremia, minimal-absent neural infection, mild-moderate neuropathology, and the lack of mortality, even though productive virus replication occurred in the draining lymph node. The kinetics of anti-WNV neutralizing antibody response was comparable to that commonly seen in infected horses and humans. This may be explained by the early IFNα/β and/or γ response evident in the draining popliteal lymph node. Given this similarity to the human and equine disease, immunocompetent rabbits are, therefore, a valuable animal model for investigating various aspects of non-lethal WNV infections.

  12. Effect of subcutaneously administered diclazuril on the output of Eimeria species oocysts by experimentally infected rabbits.

    Science.gov (United States)

    Pan, B L; Zhang, Y F; Suo, X; Xue, Y

    2008-02-02

    The effect of subcutaneously injected diclazuril on the output of Eimeria species oocysts was studied in experimentally infected rabbits. Diclazuril was administered either prophylactically at 0.5, 1 or 2 mg/kg bodyweight two days before each rabbit was inoculated with 20,000 oocysts of a mixed-species field isolate of Eimeria or therapeutically at 1, 2 or 4 mg/kg bodyweight five days after they were inoculated. The prophylactic treatments significantly reduced (PEimeria stiedae, Eimeria magna, Eimeria irresidua, Eimeria flavescens, Eimeria piriformis and Eimeria intestinalis was significantly reduced (P<0.05) in all the treated groups. The burden of oocysts in the livers of the therapeutic groups (4000 to 9000) were significantly lower (P<0.05) than in the inoculated but untreated control group (23,000), but higher than in the prophylactic groups (around 1000).

  13. Panton-Valentine leukocidin does play a role in the early stage of Staphylococcus aureus skin infections: a rabbit model.

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    Urszula Lipinska

    Full Text Available Despite epidemiological data linking necrotizing skin infections with the production of Panton-Valentine leukocidin (PVL, the contribution of this toxin to the virulence of S. aureus has been highly discussed as a result of inconclusive results of in vivo studies. However, the majority of these results originate from experiments using mice, an animal species which neutrophils--the major target cells for PVL--are highly insensitive to the action of this leukocidin. In contrast, the rabbit neutrophils have been shown to be as sensitive to PVL action as human cells, making the rabbit a better experimental animal to explore the PVL role. In this study we examined whether PVL contributes to S. aureus pathogenicity by means of a rabbit skin infection model. The rabbits were injected intradermally with 10(8 cfu of either a PVL positive community-associated methicillin-resistant S. aureus isolate, its isogenic PVL knockout or a PVL complemented knockout strain, and the development of skin lesions was observed. While all strains induced skin infection, the wild type strain produced larger lesions and a higher degree of skin necrosis compared to the PVL knockout strain in the first week after the infection. The PVL expression in the rabbits was indirectly confirmed by a raise in the serum titer of anti-LukS-PV antibodies observed only in the rabbits infected with PVL positive strains. These results indicate that the rabbit model is more suitable for studying the role of PVL in staphylococcal diseases than other animal models. Further, they support the epidemiological link between PVL producing S. aureus strains and necrotizing skin infections.

  14. Efficacy of levamisole alone and in combination with mebendazole against Gongylonema pulchrum infection in rabbits.

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    Kudo, Noboru; Ishikawa, Natsumi; Yamane, Aki; Ikadai, Hiromi; Oyamada, Takashi

    2015-01-01

    Gongylonema pulchrum is an important parasite of captive primates. Twelve rabbits were infected with 30 third-stage larvae of G. pulchrum. At 4-7 months post-infection, animals were administered levamisole at a single dose of 12 mg/kg, levamisole at 8 mg/kg three times at 2-day intervals, levamisole at a single dose of 8 mg/kg after administration of mebendazole at 70 mg/kg for 3 days or 8 ml of distilled water for 3 days (control). Necropsy at 14 days after treatment revealed that single and multiple dosages of levamisole reduced nematode burdens by 68.4% and 89.5%, respectively. The combined regimen of mebendazole and levamisole exhibited high efficacy for treating G. pulchrum located widely within the upper digestive tract, with a reduction of 98.2%. These results suggest that this combined chemotherapy treatment may be effective against G. pulchrum infection, including buccal and lingual gongylonemiasis in primates.

  15. Circular Herpesvirus sylvilagus DNA in spleen cells of experimentally infected cottontail rabbits.

    Science.gov (United States)

    Medveczky, P; Kramp, W J; Sullivan, J L

    1984-01-01

    Cottontail rabbits (Sylvilagus floridanus) were infected with Herpesvirus sylvilagus, and spleen cells were analyzed for the presence of virus-specific, covalently closed circular, and linear DNA molecules by a simple electrophoretic technique, followed by transfer to nitrocellulose filters and hybridization with cloned viral DNA (Gardella et al., J. Virol. 50:248-254, 1984). Approximately 0.2 copies per cell of circular DNA and 0.2 copies per cell of linear DNA were detected by hybridization with a cloned viral DNA fragment. The size of the viral DNA was estimated at ca. 158 kilobase pairs. Restriction endonuclease patterns suggested structural similarities to cottontail herpesvirus DNA. Images PMID:6092696

  16. Apoptosis of granulocytes and lymphocytes in peripheral blood in rabbits infected with haemagglutinating and non-haemagglutinating antigenic variants of the RHD (rabbit haemorrhagic disease) virus.

    Science.gov (United States)

    Niedźwiedzka-Rystwej, P; Tokarz-Deptuła, B; Deptuła, W

    2013-01-01

    This paper attempts to study the dynamics of apoptosis of granulocytes and lymphocytes in peripheral blood in rabbits infected with haemagglutinating (Vt97, Triptis, Hartmannsdorf) and non-haemagglutinating (Pv97, 9905 RHDVa) antigenic variants of the RHD virus. The pathogenicity of those antigenic variants was also assessed by recording the mortality of the infected animals. The animals were infected with antigenic variants and blood was sampled at hour 0, 4, 8, 12, 24, 36 p.i. and the percentage of apoptotic granulocytes and lymphocytes was measured with the use of flow cytometry. The results of the study showed that apoptosis is included during RHDV infection, as the number of apoptotic granulocytes and lymphocytes increases throughout the experiment; depending on the antigenic variant, apoptosis joins in at 4-8-12 h p.i. and lasts until 24-36 h p.i. Furthermore, the mortality of rabbits infected with the examined strains of RHD virus varied from 30% to 100%. This study performed for the first time in this manner, indicates the importance of apoptosis during infection with the RHD virus.

  17. Isolation of Trichophyton mentogrophytes var mentogrophytes from naturally infected laboratory albino rats: experimental infection and treatment in rabbits

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    N. A. Issa

    2009-01-01

    Full Text Available The present study demonstrated for the first time the occurrence of dermatophytosis in naturally infected rats and from asymptomatic and from breeding boxes of white rats kept in animal housing of college of Veterinary Medicine, University of Dohuk, Iraq. The prevalence rate of infection was (28%, clinically infected rats characterized by appearance of scaly ovoid type lesions with crusty edge and patch of hair loss mostly seen on the back, neck and face of the infected rats, itching was reported in some rats. Only one species of the trichophyton, T. mentogrophytes var mentogrophytes was isolated with growth rate (85.71% of samples collected from clinically infected rats, and (28.57% from asymptomatic and from breeding cages, the growth was observed within the 21 days at 25ºC on Sabouraud's Dextrose Agar. Lacto phenol cotton blue staining slides of T. mentogrophytes var mentogrophytes revealed both microconidia and macroconidia. Microconidia found in numerous numbers often in dense cluster which were hyaline, smooth walled and predominantly spherical to sub spherical in shape, varying numbers of chlamydoconidia. Spiral hyphae and smooth, thin walled clavate shaped multicelled macroconidia were also present. The study also dealt with experimental infection in rabbits with T. mentogrophytes var mentogrophytes and treated by two drugs, natural herbal preparation of acidic pomegranate (Punica granatum fruit and synthetic nystatine ointment. The complete recovery of lesions was recorded after 14 days and 21 days of topical application of a pomegranate and nystatine ointment for 5 successive days respectively.

  18. Evidence of Leishmania infantum Infection in Rabbits (Oryctolagus cuniculus in a Natural Area in Madrid, Spain

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    Nerea García

    2014-01-01

    Full Text Available Leishmaniasis is one of the most important neglected zoonosis and remains endemic in at least 88 developing countries in the world. In addition, anthropogenic environmental changes in urban areas are leading to its emergency world wide. Zoonotic leishmaniasis control might only be achieved by an integrated approach targeting both the human host and the animal reservoirs, which in certain sylvatic cycles are yet to be identified. Recently, hares have been pointed out as competent reservoirs of Leishmania infantum in Spain, but the role of other lagomorphs has not been clarified. Here, 69 rabbits (Oryctolagus cuniculus from a natural area in Madrid in which a high density was present were analyzed using indirect (immunofluorescence antibody test, IFAT and direct (PCR, culture techniques. Fifty-seven (82.6% of the animals were positive to at least one technique, with IFAT yielding the highest proportion of positive samples. L. infantum was isolated in 13% animals demonstrating the occurrence of infection in this setting. Our results suggest that rabbits could play a role of competent reservoir of L. infantum and demonstrate that the prevalence of infection is high in the analyzed area.

  19. White and red blood cell picture in rabbits experimentally infected with strains of the rabbit haemorrhagic disease (RHD) virus without or with variable haemagglutination capacity.

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    Niedźwiedzka-Rystwej, P; Tokarz-Deptuła, B; Deptuła, W

    2016-12-01

    The aim of the study was to establish if haemagglutination of rabbit haemorrhagic disease virus (RHDV) affects haematological picture of peripheral blood in rabbits and the pathogenicity of the virus. The study analyzed white and red blood cell picture in rabbits experimentally infected with two non-haemagglutinating (HA-) RHDV strains (Frankfurt and Asturias) and one strain with variable haemagglutination capacity (HA+/-) (Hagenow). Studies with HA- and HA +/- are rare and relate only to 4 HA- strains (2 RHDV: BLA and Rainham; 2 RHDVa: Pv97 and 9905) and 1 HA+/- RHDV strain: ŻD, where less changes in haematological indices and less pathogenicity were observed. We found that changes caused by HA- Frankfurt strain were related to the number of neutrophils and thrombocytes, while in HA- strain Asturias, in thrombocytes and leukocytes. Changes evoked by HA+/- Hagenow strain pertained to the number of eosinophils, thrombocytes, leukocytes, monocytes, and concentration of hemoglobin. Mortality caused by the Frankfurt strain was 100% between 36 and 48 h post infection (p.i.), while that caused by Asturias strain was 100% between 24 and 36 h p.i., and that observed in case of Hagenow strain was 90% between 36 and 48 h p.i. The changes in haematological picture caused by the HA- and HA+/- RHDV strains were less intensive than those found in case of the HA+ RHDV strains, which cannot be confirmed for pathogenicity, and is not in line with the existing hypothesis suggesting higher pathogenicity in HA+ viruses.

  20. Pharmacokinetics of gallium maltolate in Lawsonia intracellularis-infected and uninfected rabbits.

    Science.gov (United States)

    Sampieri, F; Alcorn, J; Allen, A L; Clark, C R; Vannucci, F A; Pusterla, N; Mapes, S; Ball, K R; Dowling, P M; Thompson, J; Bernstein, L R; Gebhart, C J; Hamilton, D L

    2014-10-01

    Oral gallium maltolate (GaM) pharmacokinetics (PK) and intestinal tissue (IT) concentrations of elemental gallium ([Ga]) and iron ([Fe]) were investigated in a rabbit model of equine proliferative enteropathy (EPE). New Zealand white does (uninfected controls and EPE-infected, n = 6/group) were given a single oral GaM dose (50 mg/kg). Serial blood samples were collected from 0 to 216 h post-treatment (PT) and IT samples after euthanasia. Serology, qPCR, and immunohistochemistry confirmed, or excluded, EPE. Blood and IT [Ga] and [Fe] were determined using inductively coupled plasma-mass spectrometry. PK parameters were estimated through noncompartmental approaches. For all statistical comparisons on [Ga] and [Fe] α = 5%. The Ga log-linear terminal phase rate constant was lower in EPE rabbits vs. uninfected controls [0.0116 ± 0.004 (SD) vs. 0.0171 ± 0.0028 per hour; P = 0.03]; but half-life (59.4 ± 24.0 vs. 39.4 ± 10.8 h; P = 0.12); Cmax (0.50 ± 0.21 vs. 0.59 ± 0.42 μg/mL; P = 0.45); tmax (1.75 ± 0.41 vs. 0.9 ± 0.37 h; P = 0.20); and oral clearance (6.743 ± 1.887 vs. 7.208 ± 2.565 L/h; P = 0.74) were not. IT's [Ga] and [Fe] were higher (P < 0.0001) in controls. In conclusion, although infection reduces IT [Ga] and [Fe], a 48 h GaM dosing interval is appropriate for multidose studies in EPE rabbits.

  1. Value of histopathology, immunohistochemistry, and real-time polymerase chain reaction in the confirmatory diagnosis of Encephalitozoon cuniculi infection in rabbits.

    Science.gov (United States)

    Leipig, Miriam; Matiasek, Kaspar; Rinder, Heinz; Janik, Dirk; Emrich, Daniela; Baiker, Kerstin; Hermanns, Walter

    2013-01-01

    Morphological lesions in kidneys and brain are all too often considered diagnostic for confirmation of encephalitozoonosis in rabbits. The current study evaluated the diagnostic value of histology versus other etiological tests, including immunohistochemistry and real-time polymerase chain reaction (PCR) for Encephalitozoon cuniculi infection diagnosis. Samples of brain, heart, lungs, intestine, liver, and kidneys from 81 rabbits were examined for morphological lesions attributed to E. cuniculi infection as well as for the presence of spores and E. cuniculi antigen. Of these, 55 rabbits were tested for E. cuniculi DNA. Histological changes consistent with E. cuniculi infection were seen in 33 rabbits (41%, 33/81) representing 87% (33/38) of all rabbits with confirmed E. cuniculi infection. Brains of these rabbits displayed 6 different types of focal lesions corresponding to the stage of infection and specific tissue response. In 5 rabbits that were tested positive, histology was either inconclusive or inconspicuous. Etiological diagnosis was based on histological spore detection in 16% (6/38) of infected rabbits. Immunohistochemistry was more sensitive (42%, 16/38) than histological spore detection, and real-time PCR proved to be the most sensitive of all investigated methods (30/35, 86% of the examined rabbits with E. cuniculi infection). Encephalitozoon cuniculi infection rarely occurs without characteristic kidney and brain lesions. However, the spectrum of brain changes is wider than previously reported. Based on these findings, confirmation of pathogenic E. cuniculi infection should include standard histology of the predilection sites and a specific etiological assay, preferably real-time PCR.

  2. Detection of Genotype 4 Swine Hepatitis E Virus in Systemic Tissues in Cross-Species Infected Rabbits

    Science.gov (United States)

    Wu, Qiaoxing; An, Junqing; She, Ruiping; Shi, Ruihan; Hao, Wenzhuo; Soomro, MajidHussain; Yuan, Xuerui; Yang, Jinling; Wang, Jingyuan

    2017-01-01

    Increasing evidence demonstrates that hepatitis E virus (HEV) can be transmitted across species. According to previous reports, swine HEV has two genotypes, genotype 3 and 4, and both can infect humans by the fecal-oral route. Thus, it is crucial for the control of HEV zoonotic transmission to evaluate the dynamics of viral shedding and distribution in different tissues during cross-species infection by HEV. In this study, rabbits were infected with genotype 4 swine HEV by the intraperitoneal route. The results showed that HEV RNA not only shed in the feces but also in the saliva of some rabbits during infection with swine HEV. Viremia appeared late after infection, and anti-HEV IgG was not obvious until the appearance of high viremia levels. After the rabbits were euthanized, a histopathological examination showed that the livers developed overt hepatitis accompanied by an elevation of alanine aminotransferase (ALT) and aspartate transaminase (AST). Furthermore, HEV RNA was detected in various tissues, especially in the salivary glands and tonsils. Subsequently, negative-stranded HEV RNA was practiced in tissues with positive HEV RNA, which demonstrated that HEV replicated in the tissues. Next, we harvested additional tissues from the liver, salivary gland, tonsil, spleen, thymus gland, lymph node and intestine, which are known as replication sites of swine HEV. Additionally, we also observed the HEV antigen distributed in the organs above through immunohistochemical staining. These results demonstrate that rabbits could be used as an animal model for researching cross-species infection of genotype 4 HEV. It is also noteworthy that HEV can shed in the saliva and presents the risk of droplet transmission. These new data provide valuable information for understanding cross-species infection by HEV. PMID:28129390

  3. Dexamethasone-induced reactivation of bovine herpesvirus type 5 latent infection in experimentally infected rabbits results in a broader distribution of latent viral DNA in the brain

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    S.V. Mayer

    2006-03-01

    Full Text Available Bovine herpesvirus type 5 (BHV-5 is a major agent of meningoencephalitis in cattle and establishes latent infections mainly in sensory nerve ganglia. The distribution of latent BHV-5 DNA in the brain of rabbits prior to and after virus reactivation was studied using a nested PCR. Fifteen rabbits inoculated intranasally with BHV-5 were euthanized 60 days post-inoculation (group A, N = 8 or submitted to dexamethasone treatment (2.6 mg kg-1 day-1, im, for 5 days and euthanized 60 days later (group B, N = 7 for tissue examination. Two groups of BHV-1-infected rabbits (C, N = 3 and D, N = 3 submitted to each treatment were used as controls. Viral DNA of group A rabbits was consistently detected in trigeminal ganglia (8/8, frequently in cerebellum (5/8, anterior cerebral cortex and pons-medulla (3/8 and occasionally in dorsolateral (2/8, ventrolateral and posterior cerebral cortices, midbrain and thalamus (1/8. Viral DNA of group B rabbits showed a broader distribution, being detected at higher frequency in ventrolateral (6/7 and posterior cerebral cortices (5/7, pons-medulla (6/7, thalamus (4/7, and midbrain (3/7. In contrast, rabbits inoculated with BHV-1 harbored viral DNA almost completely restricted to trigeminal ganglia and the distribution did not change post-reactivation. These results demonstrate that latency by BHV-5 is established in several areas of the rabbit's brain and that virus reactivation leads to a broader distribution of latent viral DNA. Spread of virus from trigeminal ganglia and other areas of the brain likely contributes to this dissemination and may contribute to the recrudescence of neurological disease frequently observed upon BHV-5 reactivation.

  4. Real time PCR detection of rabbit haemorrhagic disease virus in rabbits infected with different European strains of RHDV.

    Science.gov (United States)

    Niedźwiedzka-Rystwej, P; Hukowska-Szematowicz, B; Działo, J; Tokarz-Deptuła, B; Deptuła, W

    2013-01-01

    The paper concerns the use of a novel, very effective diagnostic method, a real-time PCR for diagnosis of a viral agent causing viral haemorrhagic disease in rabbits - RHDV. Until now, the method was widely used for detecting many different viruses, both DNA, and RNA, but as far as RHDV is concerned, there are not many records of such use. This study aimed at the detection of 17 different strains from different European regions, differing in biological features and mortality. The study confirmed that real-time PCR is an applicable and effective method for diagnosis of RHDV, irrespective of the stains' features.

  5. A thymidine kinase-negative bovine herpesvirus 5 is highly attenuated for rabbits, but is neuroinvasive and establishes latent infection

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    Sara Campos da Silva

    2011-05-01

    Full Text Available Mutant viral strains deleted in non-essential genes represent useful tools to study the function of specific gene products in the biology of the virus. We herein describe an investigation on the phenotype of a bovine herpesvirus 5 (BoHV-5 recombinant deleted in the gene encoding the enzyme thymidine kinase (TK in rabbits, with special emphasis to neuroinvasiveness and the ability to establish and reactivate latent infection. Rabbits inoculated with the parental virus (SV-507/99 (n=18 at a low titer (10(5.5TCID50 shed virus in nasal secretions in titers up to 10(4.5TCID50 for up to 12 days (average: 9.8 days [5-12] and 5/ 16 developed neurological disease and were euthanized in extremis. Rabbits inoculated with the recombinant BoHV-5TKΔ at a high dose (10(7.1TCID50 also shed virus in nasal secretions, yet to lower titers (maximum: 10(2.3TCID50 and for a shorter period (average: 6.6 days [2-11] and remained healthy. PCR examination of brain sections of inoculated rabbits at day 6 post-infection (pi revealed a widespread distribution of the parental virus, whereas DNA of the recombinant BoHV-5TKΔ-was detected only in the trigeminal ganglia [TG] and olfactory bulbs [OB]. Nevertheless, during latent infection (52pi, DNA of the recombinant virus was detected in the TGs, OBs and also in other areas of the brain, demonstrating the ability of the virus to invade the brain. Dexamethasone (Dx administration at day 65 pi was followed by virus reactivation and shedding by 5/8 rabbits inoculated with the parental strain (mean duration of 4.2 days [1 - 9] and by none of seven rabbits inoculated with the recombinant virus. Again, PCR examination at day 30 post-Dx treatment revealed the presence of latent DNA in the TGs, OBs and in other areas of the brain of both groups. Taken together, these results confirm that the recombinant BoHV-5TKΔ is highly attenuated for rabbits. It shows a reduced ability to replicate in the nose but retains the ability to invade

  6. Dynamic observation of polypide in semen and blood of rabbits infected with Toxoplasma tachyzoites

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Toxoplasma gondii (T. gondii) is one of the intracellular parasitized protozoa and may cause severe medical complications in fetus or immunocompromised individuals. T. gondii existed as tachyzoite during acute stage while as bradyzoite during chronic phase in human cells. To improve understanding of the prevention, diagnosis, and treatment of the disease, it is important to explore the distribution and fluctuation and other biological features of T. gondii in host. The trophozoite had been found in the saliva, blood or urine of the host.1-4 Some studies suggested the dynamic changes of circulating antibody and toxoplasma circulating antigen (TCA) either in blood or in urine.5,6 T. gondii in tissue or blood cannot be counted exactly under the microscope because it was only several micrometers in size and thus most of the studies were performed qualitatively by mouse inoculation or immunology methods. The quantitative fluorescent polymerase chain reaction (QF-PCR) and its application raised the possibility for dynamic observation of the polypide in the host.7,8 In this study, blood and semen were collected from the male rabbit model infected with toxoplasma tachyzoites and T. gondii was detected by QF-PCR quantitatively.

  7. The effect of single and concomitant pathogen infections on condition and fecundity of the wild rabbit (Oryctolagus cuniculus).

    Science.gov (United States)

    Lello, J; Boag, B; Hudson, P J

    2005-12-01

    We assessed the effect of two pathogens (myxoma virus and Eimeria stiedae) and five macroparasites (gastrointestinal helminth species) of the wild rabbit (Oryctolagus cuniculus) upon total host body mass and abdominal fat level. Additionally, we assessed the effects of these organisms on the number of foetuses in adult females during the peak breeding period. Both mass of abdominal fat and total body mass of the rabbit were negatively associated with myxoma virus infection and increasing helminth species richness. Total body mass was also negatively associated with the protozoan parasite E. steidae. No relationship was found between any of the parasites/pathogens and the number of foetuses in adult females, although only relatively small sample sizes were available for this section of the analysis. Increasing host body mass was positively associated with number of foetuses and we propose that mass reduction caused by the pathogen and parasite species could also have the consequence of reducing foetal number.

  8. Sequential changes in susceptibility to Treponema pallidum of rabbits previously infected with Treponema paraluis-cuniculi

    OpenAIRE

    Graves, S.

    1981-01-01

    Rabbits immunised with virulent Treponema paraluis-cuniculi were challenged intradermally with graded doses of Treponema pallidum at three, five, seven, 12, and 30 months to ascertain the level of protection to T pallidum at various intervals after immunisation.

  9. Ultrastructural Pathologic Observation on the Gut-Associated Lymphoid Tissues of Sacculus Rotundus of Rabbits Infected with Rabbit Haemorrhagic Disease Virus

    Institute of Scientific and Technical Information of China (English)

    SHE Rui-ping; YANG Han-chun; JIA Jun-zheng; LIU Hai-hong; MA Yi-xin; Itakura C

    2003-01-01

    Ultrastructural pathological changes in the gut-associated lymphoid tissues of sacculus rotundus(SR) of rabbits infected with rabbit haemorrhagic disease virus (RHDV) were first observed. There were nu-merous holes at the luminal and basement membrane surfaces of the dome epithelium(DE), consistently ac-companied by necrosis of lymphocytes and M-cells, and pronounced depletion of lymphocytes in the domes andfollicles, decrease of DE complex with formation of pseudomembranous structure on the surface of the domeepithelium. A specific finding in lymphocytes and macrophages was that severe destruction detraction of themembrane of rough endoplasmic reticulum(RER) was accompanied by conspicious increase of solitary, ribo-some-like particles in the cytoplasm, with appearances of intranuclear particles and intranuclear inclusions. Itwas found that there were many round and dense virion-like particles, with 26 nm in diameter, in the nucleiand cytoplasm of lymphoctes, plasma cells, macrophages and fibroblasts, or in degenerated cells and cellulardebris. At the same time, another round virion-like particles about 34 nm in diameter were also seen in the cy-toplasm of some cells and interstitium. The results indicated that the appearances of the ribosome-like parti-cles, virion-like particles and inclusion bodies were related to the replication and assembly of RHDV. Thepresent observations suggested that DE of sacculus rotundus could be a open pathway and a transporting routefor the entry of antigens into hosts. While the antigen is profoundly deleterious, DE may be as a closed portalor a barrier preventing the foreign antigenic materials from invading.

  10. INFECÇÃO EXPERIMENTAL POR Trypanosoma evansi EM COELHOS EXPERIMENTAL INFECTION BY Trypanosoma evansi IN RABBITS

    Directory of Open Access Journals (Sweden)

    Silvia Gonzalez Monteiro

    2008-07-01

    Full Text Available

    Avaliou-se o período pré-patente e a evolução da parasitemia de coelhos infectados experimentalmente por Trypanosoma evansi. Dividiram-se coelhos machos, com idade entre dois a seis meses, em quatro grupos (A, B, C, D com três animais cada. Inocularam-se os coelhos dos grupos A, B e C com T. evansi e procedeu-se à avaliação diária, mediante esfregaços sangüíneos periféricos, observando-se que o período pré-patente nos grupos infectados (A, B e C foi de seis, sete e onze dias, respectivamente. Infectaram-se os animais do grupo A com T. evansi e Eimeria spp. e eles tiveram uma longevidade de 56 dias após o início do trabalho. Já os animais do grupo B, infectados com T. evansi e tratados com coccidiostático, apresentaram longevidade semelhante ao grupo C, que continha coelhos parasitados somente por T. evansi. Nesses dois grupos a parasitemia manteve-se com picos irregulares em variações de zero a cinco tripomastigotas/campo por até 89 dias após a inoculação, desaparecendo da circulação após 90 dias. Os coelhos do grupo D, grupo-controle, não foram infectados. Os animais dos grupos B, C e D foram sacrificados após 120 dias do início do experimento.

     

    Palavras-chaveS: Coelho, parasitemia, Trypanosoma evansi.

    This work had the aim to evaluate the pre-patent period and the evolution of the parasitemy of rabbits experimentally infected by Trypanosoma evansi. Male rabbits from two to six months were divided in four groups of three animals each (A, B, C, D. Group A, B and C rabbits were infected with T. evansi and evaluated daily through peripheral blood smears. The daily analyses showed a pre-patent period in the infected groups (A, B and C of six, seven and 11 days, respectively. Animals from group A, infected by T. evansi

  11. A resorbable antibiotic-eluting polymer composite bone void filler for perioperative infection prevention in a rabbit radial defect model.

    Directory of Open Access Journals (Sweden)

    Benjamin D Brooks

    Full Text Available Nearly 1.3 million total joint replacement procedures are performed in the United States annually, with numbers projected to rise exponentially in the coming decades. Although finite infection rates for these procedures remain consistently low, device-related infections represent a significant cause of implant failure, requiring secondary or revision procedures. Revision procedures manifest several-fold higher infection recurrence rates. Importantly, many revision surgeries, infected or not, require bone void fillers to support the host bone and provide a sufficient tissue bed for new hardware placement. Antibiotic-eluting bone void fillers (ABVF, providing both osteoconductive and antimicrobial properties, represent one approach for reducing rates of orthopedic device-related infections. Using a solvent-free, molten-cast process, a polymer-controlled antibiotic-eluting calcium carbonate hydroxyapatite (HAP ceramic composite BVF (ABVF was fabricated, characterized, and evaluated in vivo using a bacterial challenge in a rabbit radial defect window model. ABVF loaded with tobramycin eliminated the infectious burden in rabbits challenged with a clinically relevant strain of Staphylococcus aureus (inoculum as high as 10⁷ CFU. Histological, microbiological, and radiographic methods were used to detail the effects of ABVF on microbial challenge to host bone after 8 weeks in vivo. In contrast to the HAP/BVF controls, which provided no antibiotic protection and required euthanasia 3 weeks post-operatively, tobramycin-releasing ABVF animals showed no signs of infection (clinical, microbiological, or radiographic when euthanized at the 8-week study endpoint. ABVF sites did exhibit fibrous encapsulation around the implant at 8 weeks. Local antibiotic release from ABVF to orthopedic sites requiring bone void fillers eliminated the periprosthetic bacterial challenge in this 8-week in vivo study, confirming previous in vitro results.

  12. Development of a SYBR-based real-time PCR to detect rabbit hemorrhagic disease virus(RHDV) and analyze its tissue distribution in experimentally infected rabbits

    Institute of Scientific and Technical Information of China (English)

    Wenkai; Liu; Ruyi; Dang; Xinglong; Wang

    2015-01-01

    <正>Dear Editor,Rabbit hemorrhagic disease(RHD)is a highly contagious disease of both wild and domesticated rabbits(Oryctolagus cuniculus).The causative agent of the disease is the rabbit hemorrhagic disease virus(RHDV),belongs to the genus Lagovirus within the family Caliciviridae(Granzow et al.,1996;Ohlinger et al.,1990).It is a small and non-enveloped virus with a 7.5 kb single stranded positive sense RNA genome(Meyers et al.,1991;Meyers et al.,2000).Based on an analysis of VP60

  13. Autoantibody Production in Rabbits

    Science.gov (United States)

    Asherson, G. L.; Rose, M. Elaine

    1963-01-01

    The finding that the serum of apparently healthy rabbits fixed complement with rabbit liver and kidney has been confirmed. Experimental infection of rabbits with Eimeria stiedae, the cause of hepatic coccidiosis, led to a rise in the titre of serum complement-fixing factors. The rise was statistically significant 14, 21 and 28 days after infection. The factors were regarded as antibodies because they behaved as macroglobulins on diethylaminoethyl—cellulose chromatography and sucrose gradient centrifugation, and as autoantibodies because they fixed complement with the kidney of the rabbits in which they occurred. The antibody reacted with widely distributed antigen(s) with high activity in brain and low activity in skeletal muscle. The possibility that coccidial infection may be responsible for the natural autoantibody of rabbits is discussed. PMID:13965167

  14. Seroprevalence and Risk Factors of Chlamydia Infection in Domestic Rabbits (Oryctolagus cuniculus) in China

    OpenAIRE

    Xiaoting Ni; Siyuan Qin; Zhilong Lou; Hongrui Ning; Xiaolin Sun

    2015-01-01

    Chlamydia spp. are obligate intracellular bacteria distributed all over the world, known to cause various forms of diseases in animals and humans. In the present study, a serological survey was conducted to detect the seroprevalence and risk factors associated with rabbit chlamydiosis in northeast China, including Liaoning province, Jilin province, Heilongjiang province, and Inner Mongolia Autonomous Region. Antibodies to Chlamydia were determined by indirect hemagglutination assay (IHA). The...

  15. Altering the motility of Trypanosoma cruzi with rabbit polyclonal anti-peptide antibodies reduces infection to susceptible mammalian cells.

    Science.gov (United States)

    Finkelsztein, Eli J; Diaz-Soto, Juan C; Vargas-Zambrano, Juan C; Suesca, Elizabeth; Guzmán, Fanny; López, Manuel C; Thomas, M Carmen; Forero-Shelton, Manu; Cuellar, Adriana; Puerta, Concepción J; González, John M

    2015-03-01

    Trypanosoma cruzi's trypomastigotes are highly active and their incessant motility seems to be important for mammalian host cell infection. The kinetoplastid membrane protein-11 (KMP-11) is a protein expressed in all parasite stages, which induces a cellular and humoral immune response in the infected host, and is hypothesized to participate in the parasite's motility. An N-terminal peptide from KMP-11, termed K1 or TcTLE, induced polyclonal antibodies that inhibit parasitic invasion of Vero cells. The goal of this study was to evaluate the motility and infectivity of T. cruzi when exposed to polyclonal anti-TcTLE antibodies. Rabbits were immunized with TcTLE peptide along with FIS peptide as an immunomodulator. ELISA assay results showed that post-immunization sera contained high titers of polyclonal anti-TcTLE antibodies, which were also reactive against the native KMP-11 protein and live parasites as detected by immunofluorescence and flow cytometry assays. Trypomastigotes of T. cruzi were incubated with pre- or post-immunization sera, and infectivity to human astrocytes was assessed by Giemsa staining/light microscope and flow cytometry using carboxyfluorescein diacetate succinimidyl ester (CFSE) labeled parasites. T. cruzi infection in astrocytes decreased approximately by 30% upon incubation with post-immunization sera compared with pre-immunization sera. Furthermore, trypomastigotes were recorded by video microscopy and the parasite's flagellar speed was calculated by tracking the flagella. Trypomastigotes exposed to post-immunization sera had qualitative alterations in motility and significantly slower flagella (45.5 µm/s), compared with those exposed to pre-immunization sera (69.2 µm/s). In summary, polyclonal anti-TcTLE serum significantly reduced the parasite's flagellar speed and cell infectivity. These findings support that KMP-11 could be important for parasite motility, and that by targeting its N-terminal peptide infectivity can be reduced.

  16. Cefazolin-containing poly(ε-caprolactone sponge pad to reduce pin tract infection rate in rabbits

    Directory of Open Access Journals (Sweden)

    Hirotaka Mutsuzaki

    2014-04-01

    Full Text Available In our previous study, a fibroblast growth factor-2 (FGF-2–apatite composite layer coated on titanium screws effectively prevented pin tract infection in rabbits because of enhanced wound healing; however, the FGF-2–apatite composite layers did not completely prevent pin tract infection. Thus, we recently developed a poly(ε-caprolactone (PCL sponge pad embedded with cefazolin sodium (+CEZ, which has a fast-acting bactericidal effect. The pad is placed on the skin around the screws. The purpose of this study was to determine the anti-infective efficacy of the +CEZ pad on the pin–skin interface of the FGF-2–apatite-coated titanium screws. The +CEZ pads were prepared by mixing PCL and CEZ in 1,4-dioxane, followed by freeze-drying and compaction. They were analyzed regarding their surface structure, in vitro CEZ release profile, and bactericidal activity. The FGF-2–apatite-coated screws were implanted percutaneously in bilateral rabbit proximal tibial metaphyses—with and without the +CEZ pad—for 4 weeks (n = 20. The + CEZ pads consisted of a porous matrix of PCL in which CEZ was embedded. The CEZ-release profile showed an initial burst on Day 1 and a sustained release lasting for 30 days. The +CEZ pad retained its bactericidal activity against Staphylococcus aureus after preincubation on an agar plate for 7 days. Based on visual inspection, the pin tract infection rate was successfully reduced from 72.2% to 15.0% with the +CEZ pad (p < 0.05, which reduced the bacterial count, especially S. aureus (p < 0.05. The histological inflammation rate of the soft tissues was also significantly lower with the +CEZ pad than without it (p < 0.05. The pin tract infection rate was reduced to one-fifth with the +CEZ pad. Using it as described improves infection resistance during percutaneous implantation.

  17. Early infections by myxoma virus of young rabbits (Oryctolagus cuniculus) protected by maternal antibodies activate their immune system and enhance herd immunity in wild populations.

    Science.gov (United States)

    Marchandeau, Stéphane; Pontier, Dominique; Guitton, Jean-Sébastien; Letty, Jérôme; Fouchet, David; Aubineau, Jacky; Berger, Francis; Léonard, Yves; Roobrouck, Alain; Gelfi, Jacqueline; Peralta, Brigitte; Bertagnoli, Stéphane

    2014-03-04

    The role of maternal antibodies is to protect newborns against acute early infection by pathogens. This can be achieved either by preventing any infection or by allowing attenuated infections associated with activation of the immune system, the two strategies being based on different cost/benefit ratios. We carried out an epidemiological survey of myxomatosis, which is a highly lethal infectious disease, in two distant wild populations of rabbits to describe the epidemiological pattern of the disease. Detection of specific IgM and IgG enabled us to describe the pattern of immunity. We show that maternal immunity attenuates early infection of juveniles and enables activation of their immune system. This mechanism associated with steady circulation of the myxoma virus in both populations, which induces frequent reinfections of immune rabbits, leads to the maintenance of high immunity levels within populations. Thus, myxomatosis has a low impact, with most infections being asymptomatic. This work shows that infection of young rabbits protected by maternal antibodies induces attenuated disease and activates their immune system. This may play a major role in reducing the impact of a highly lethal disease when ecological conditions enable permanent circulation of the pathogen.

  18. Iatrogenic hemolytic anemia and endocarditis in New Zealand white rabbits secondary to Achromobacter xylosoxidans infection.

    Science.gov (United States)

    Allison, Sarah O; Artwoh, James E; Fortman, Jeffrey D; Pogwizd, Steven; Jeanes, Jodi; Koske, Sarah; Pinkerton, Marie E; Haschek, Wanda M; Messick, Joanne

    2007-11-01

    During a 3-mo period, 9 of the 15 New Zealand White rabbits used in a heart failure study developed a hemolytic anemia. The heart failure model involved the creation of an aortic insufficiency (AI) followed 2 to 6 wk later by the creation of an aortic stenosis (AS). None of the 9 animals that developed hemolytic anemia responded to medical management, and 6 of the 9 were euthanized for humane concerns. Necropsies and blood cultures were performed on all anemic animals; 7 of these cultures yielded growth of Achromobacter xylosoxidans. In addition, cultures from the heart valves of 2 rabbits yielded growth of Achromobacter xylosoxidans. We presume that the endocarditis caused by Achromobacter xylosoxidans led to the mechanical damage of red blood cells (RBCs) and subsequent intravascular hemolysis or splenic destruction of damaged RBCs, resulting in a severe, regenerative hemolytic anemia. Achromobacter xylosoxidans is an aerobic, catalase-positive, oxidase-positive, gram-negative bacillus. This organism is an environmentally resistant and opportunistic bacterium that typically inhabits aqueous environments. Microbial samples from the investigator's laboratory and equipment were collected to identify the source of the bacteria. A pressure transducer and bag of intravenous fluid were identified as sources of contamination.

  19. The preparation of aseptic rabbit tissue cage infection model%兔组织笼无菌感染模型的制备

    Institute of Scientific and Technical Information of China (English)

    刘树玲; 丁丽; 邢昆

    2015-01-01

    目的:制备兔组织笼无菌感染模型,为药物药动学-药效学结合模型(pharmacokinetic‐pharmaco‐dynamic modeling ,PK‐PD)研究提供在体动物实验载体。方法选取健康家兔28只,耳缘静脉注射盐酸赛拉嗪注射液0.15ml/kg麻醉,每只分别背部备皮、切开皮肤,然后在皮下植入长约5cm的无菌中空胸腔引流管1个,继而在无菌操作下手术缝合。术后应用青霉素肌肉注射3d以预防感染。结果5只家兔先后因不同原因死亡,23只家兔均获得清亮的无菌组织液,成功建立兔组织模型,成功率为82.14%。结论通过严格控制无菌操作,选择合适的麻醉剂,手术刀口选择在兔背部,术后及时预防感染,可以成功制备兔组织笼无菌感染模型,并获得清亮的无菌组织液。%Objective To prepare a rabbit tissue cage model so as to provide drug animal carrier in vivo for drug PK‐PD modeling study .Methods 28 healthy rabbits were selected and injected with the anesthesia xylazine iv respectively .The back hair was cut in each rabbit ,and then the skin was cut open .A 5cm of sterile hollow chest tube drainage was implanted subcutaneously .In the end the wound was sewed up with aseptic technique .After op‐eration penicillin im was injected for 3 days to prevent infection .Results Among the 28 rabbits ,5 rabbits were dead due to different causes .Clear and sterile tissue fluid were gotten from other 23 rabbits .A rabbit tissue model was established successfully ,and the success rate was 82 .14% .Conclusion By stricted controlling aseptic opera‐tion ,selecting the appropriate anesthesia and surgical incision in the back of the rabbit ,timely postoperative anti‐infection ,a rabbit tissue cage model can be successfully established ,so does clear tissue fluid .

  20. Efficacy of the Rabbit Polyclonal Anti-leptospira Antibody against Homotype or Heterotype Leptospira Infection in Hamster

    Science.gov (United States)

    Ding, Zhuang; Wang, Hai; Wu, Dianjun; Xie, Xufeng; Lin, Tao; Fu, Yunhe; Zhang, Naisheng; Cao, Yongguo

    2016-01-01

    Leptospirosis, caused by Leptospira, is one of the most important of neglected emerging zoonotic diseases that has important impacts on public health worldwide. Polyclonal antibody (pcAb) therapy is a potential method to process a series of pathogens for which there are limited determination of treatment, such as leptospirosis. First, we evaluated the efficacy of pcAb, derived from the sera of rabbits inoculated with Leptospira, against homotype (Leptospira interrogans serovar Lai) or heterotype (Leptospira interrogans serovar Autumnalis) Leptospira infection in a lethal hamster model. The pcAb treatment improved survival compared to the controls. The histopathology’s of the infected kidney, liver and lung were also examined by hematoxylin and eosin staining. Using real-time quantitative PCR, we determined that most of the leptospires in the primary organs were almost completely removed by pcAb. In the second experiment, treatments, including antibiotic, pcAb, and combination, were started immediately after occurrence of the first serious sickness mouse in any group. No significant difference in survival rate between pcAb group and antibiotic group was found, but the combination therapy group significantly improved survival rate compared to the others (Phamster, and combination therapy improved survival compared to antibiotic group in the late treatment of homotype leptospirosis. PMID:28027297

  1. Lavage with allicin in combination with vancomycin inhibits biofilm formation by Staphylococcus epidermidis in a rabbit model of prosthetic joint infection.

    Directory of Open Access Journals (Sweden)

    Haohan Zhai

    Full Text Available BACKGROUND AND AIM: The present anti-infection strategy for prosthetic joint infections (PJI includes the use of antibiotics and surgical treatments, but the bacterial eradication rates are still low. One of the major challenges is the formation of biofilm causing poor bacterial eradication. Recently it has been reported that allicin (diallyl thiosulphinate, an antibacterial principle of garlic, can inhibit bacteria adherence and prevent biofilm formation in vitro. However, whether allicin could inhibit biofilm formation in vivo is unknown. The aim of this study was to investigate the effects of allicin on biofilm formation, and whether allicin could potentiate the bactericidal effect of vancomycin in a rabbit PJI model. METHODS: A sterile stainless-steel screw with a sterile ultra-high molecular weight polyethylene washer was inserted into the lateral femoral condyle of the right hind knee joint of rabbit, and 1 mL inoculum containing 104 colony-forming units of Staphylococcus epidermidis was inoculated into the knee joint (n = 32. Fourteen days later, rabbits randomly received one of the following 4 treatments using continuous lavages: normal saline, vancomycin (20 mcg/mL, allicin (4 mg/L, or allicin (4 mg/L plus vancomycin (20 mcg/mL. Three days later, the washer surface biofilm formation was examined by scanning electron microscopy (SEM. The bacterial counts within the biofilm of implanted screws were determined by bacterial culture. RESULTS: The lowest number of viable bacterial counts of Staphylococcus epidermidis recovered from the biofilm was in the rabbits treated with allicin plus vancomycin (P<0.01 vs. all other groups. The biofilm formation was significantly reduced or undetectable by SEM in rabbits receiving allicin or allicin plus vancomycin. CONCLUSION: Intra-articular allicincan inhibit biofilm formation and enhance the bactericidal effect of vancomycin on implant surface in vivo. Allicin in combination with vancomycin may be

  2. Efficacy of CMX001 as a Prophylactic and Presymptomatic Antiviral Agent in New Zealand White Rabbits Infected with Rabbitpox Virus, a Model for Orthopoxvirus Infections of Humans

    Directory of Open Access Journals (Sweden)

    Richard W. Moyer

    2011-01-01

    Full Text Available CMX001, a lipophilic nucleotide analog formed by covalently linking 3‑(hexdecyloxypropan-1-ol to cidofovir (CDV, is being developed as a treatment for smallpox. CMX001 has dramatically increased potency versus CDV against all dsDNA viruses and, in contrast to CDV, is orally available and has shown no evidence of nephrotoxicity in healthy volunteers or severely ill transplant patients to date. Although smallpox has been eliminated from the environment, treatments are urgently being sought due to the risk of smallpox being used as a bioterrorism agent and for monkeypox virus, a zoonotic disease of Africa, and adverse reactions to smallpox virus vaccinations. In the absence of human cases of smallpox, new treatments must be tested for efficacy in animal models. Here we first review and discuss the rabbitpox virus (RPV infection of New Zealand White rabbits as a model for smallpox to test the efficacy of CMX001 as a prophylactic and early disease antiviral. Our results should also be applicable to monkeypox virus infections and for treatment of adverse reactions to smallpox vaccination.

  3. Immunosuppression abrogates resistance of young rabbits to Rabbit Haemorrhagic Disease (RHD).

    Science.gov (United States)

    Marques, Raquel M; Teixeira, Luzia; Aguas, Artur P; Ribeiro, Joana C; Costa-e-Silva, António; Ferreira, Paula G

    2014-02-04

    Rabbit Haemorrhagic Disease (RHD) is caused by a calicivirus (RHDV) that kills 90% of infected adult European rabbits within 3 days. Remarkably, young rabbits are resistant to RHD. We induced immunosuppression in young rabbits by treatment with methylprednisolone acetate (MPA) and challenged the animals with RHDV by intramuscular injection. All of these young rabbits died within 3 days of infection due to fulminant hepatitis, presenting a large number of RHDV-positive dead or apoptotic hepatocytes, and a significant seric increase in cytokines, features that are similar to those of naïve adult rabbits infected by RHDV. We conclude that MPA-induced immunosuppression abrogates the resistance of young rabbits to RHD, indicating that there are differences in the innate immune system between young and adult rabbits that contribute to their distinct resistance/susceptibility to RHDV infection.

  4. Effect of Bacterial Infection on Proliferating Cell Nuclear Antigen Expression after Partial Splenectomy of Rabbits Using Microwave Coagulator

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ The purpose of this study was to investigate the proliferating cell nuclear antigen (PCNA) expression of preserved spleen in rabbits when pneumonia diplococcus suspension was administered after partial splenectomy using microwaver coagulator.

  5. Disposable rabbit

    Science.gov (United States)

    Lewis, Leroy C.; Trammell, David R.

    1986-01-01

    A disposable rabbit for transferring radioactive samples in a pneumatic transfer system comprises aerated plastic shaped in such a manner as to hold a radioactive sample and aerated such that dissolution of the rabbit in a solvent followed by evaporation of the solid yields solid waste material having a volume significantly smaller than the original volume of the rabbit.

  6. Disposal rabbit

    Science.gov (United States)

    Lewis, L.C.; Trammell, D.R.

    1983-10-12

    A disposable rabbit for transferring radioactive samples in a pneumatic transfer system comprises aerated plastic shaped in such a manner as to hold a radioactive sample and aerated such that dissolution of the rabbit in a solvent followed by evaporation of the solid yields solid waste material having a volume significantly smaller than the original volume of the rabbit.

  7. Congenital Transmission of Schistosoma japonicumin the Rabbit

    Institute of Scientific and Technical Information of China (English)

    QianBao-zhen; H.O.Bogh; M.V.Johansen; WangPeng-peng

    2005-01-01

    Fourteen pregnant rabbits were each infected with 300 cercariae of Schistosoma japonicum and divided into two groups.Group M (n =8)was infected during mid-gestation (the organogenetic stage)and group L (n=6)was infected during late-gestation (the post-organogenetic stage).Mother rabbits and rabbit kittens were killed 45-60 days after infection and perfused in order to obtain worm counts.Furthermore,faecal egg counts and tissue egg counts from livers were obtained from the mother rabbits as well as the rabbit kittens.All mother rabbits became infected harbouring 207.6+20.2 and 220.0+27.5 adult worms in group M and L,respectively.In groups M and L, 13.5%and 46.7% of the kittens were infected,respectively,In 12 of 14 litters at least one kitten was infected.Tne infected kittens harboured between one and three adult S.japonicum.The livers of the kittens infected with a worm pair displaced lesions,as a result of egg deposition.The results, therefore,show that congenital transmission of S.japonicum can occur in rabbits.The close anatomical resemblance between the rabbit and human placenta may be indicative of the presence of congenital transmssion of S.japomcum infection in humans.

  8. Belt conveyer

    Energy Technology Data Exchange (ETDEWEB)

    Cwieczek, A.; Dembinski, C.

    1982-04-30

    The patented belt conveyor is distinguished by the fact that the rate of motion of the belt changes smoothly depending on the load: the greater the load the higher the rate. This makes it possible to prolong the service life of the belt, i.e., during idling of the conveyor it is exposed to deformation on the drive and tension drums a fewer number of times. The essence of the invention is based on the use for driving the drum of a friction transmission. One of the elements of this transmission is the drive drum of the conveyor, and the other is the drive wheel which is pressed to the inner (or outer) surface of the drum. Change in rotation velocity of the drum is reached by changing the diameter of the drive wheel. The rim of the latter has an elastic tire to which compressed air is fed. The diameter of the drive wheel depends on the quantity of air in the tire. It is set automatically by a regulating system depending on the conveyor load. Variants are patented for the belt conveyor which is distinguished by the design of the friction transmission. It contains 1, 2 or more drive wheels. It can have a cylindrical or conical inner surface of the drive drum, etc.

  9. Naturally occurring antibodies against nerve growth factor in human and rabbit sera: comparison between control and herpes simplex virus-infected patients.

    Science.gov (United States)

    Dicou, E; Nerrière, V; Labropoulou, V

    1991-11-01

    Antibodies against nerve growth factor (NGF) in sera were detected by enzyme-linked immunosorbent assays (ELISA), by their isolation after passage of sera through NGF immunoadsorbent columns and by their specificity to bind and immunoprecipitate mouse NGF as well as to stain by immunohistochemical methods cellular sites of NGF synthesis. Increased levels of anti-NGF antibodies were found in sera of herpes simplex virus (HSV)-infected patients but not in HSV-inoculated rabbits. As HSV latency is known to be promoted by NGF in vitro, these results may suggest that anti-NGF antibodies modulate the cytokine function of NGF and thus might play a role in HSV infection. The biological function of circulating antibodies against NGF, in general, is now open to future investigation.

  10. An imaging analysis of {sup 99m}Tc-UBI (29-41) uptake in S. aureus infected thighs of rabbits on ciprofloxacin treatment

    Energy Technology Data Exchange (ETDEWEB)

    Akhtar, Muhammad S. [Punjab Institute of Nuclear Medicine (PINUM), P.O. Box-2019, Faisalabad (Pakistan); Pakistan Institute of Engineering and Applied Sciences (PIEAS) University, Islamabad (Pakistan); Khan, Muhammad E.; Irfanullah, Javaid; Afzal, Muhammad S.; Khan, Muhammad A.; Nadeem, Muhammad A.; Imran, Muhammad B. [Punjab Institute of Nuclear Medicine (PINUM), P.O. Box-2019, Faisalabad (Pakistan); Khan, Bashar; Jehangir, Mustansar [Pakistan Institute of Nuclear Science and Technology (PINSTECH), Islamabad (Pakistan)

    2008-06-15

    The uptake of {sup 99m}Tc-UBI (29-41) was evaluated at sites of bacterial infections in rabbits before and after treatment with ciprofloxacin. Staphylococcus aureus susceptible to ciprofloxacin was used to induce a focal infection in each rabbit of group 1 (G1), group 2 (G2), and group 3 (G3) with 2 x 10{sup 4}, 2 x 10{sup 6}, and 2 x 10{sup 8} colony forming units (CFU), respectively. After 24 h, images of infected thighs (target: T) and contralateral thighs (nontarget: NT) were acquired. Animals then received ciprofloxacin intramuscularly for 5 days followed by imaging on the third and fifth days. The control group 4 (G4) was imaged at days 1, 3, and 5 under the same acquisition parameters. Group 5 (G5) was employed to study biodistribution of the peptide. Increases in (T/NT) ratios in G1, G2, and G3 were observed from 5 min onwards with maximum values at 60 min. G3 revealed the highest accumulation of the peptide. Growth of the same strain of S. aureus on blood agar medium was visualized after fine needle aspiration. After ciprofloxacin treatment, the images for G1-G3 resulted in significantly decreased (P < 0.05) T/NT values on the third and fifth days that correlated with reduction in number of viable bacteria. No significant difference (P < 0.05) in left to right thigh ratios in the control group (G4) was observed. Biodistribution of the peptide showed rapid removal of tracer from circulation through the kidneys. {sup 99m} Tc-UBI (29-41) accumulation directly correlates with the number of viable bacteria. This infection localization agent can be utilized for monitoring efficacy and duration of antibiotic treatment. (orig.)

  11. Alterações hematológicas em coelhos infectados experimentalmente pelo Trypanosoma evansi Hematological changes in rabbits experimentally infected by Trypanosoma evansi

    Directory of Open Access Journals (Sweden)

    Aleksandro Schafer da Silva

    2008-04-01

    Full Text Available O objetivo deste trabalho foi avaliar as alterações hematológicas em coelhos infectados experimentalmente pelo Trypanosoma evansi. Foram utilizados seis coelhos, machos, divididos em dois grupos, sendo o grupo controle composto de animais não infectados e o grupo teste formado por roedores infectados com T. evansi. Os animais foram mantidos em temperatura ambiente e analisados por 120 dias, através de cinco coletas de sangue nos dias 1, 20, 40, 60 e 120. As alterações hematológicas foram observadas na segunda e terceira coletas de sangue. Nos animais com parasitemia, foi observada uma redução no número de hemácias, de concentração de hemoglobina no hematócrito, na concentração de hemoglobina corpuscular média (CHCM, e um aumento do volume corpuscular médio (VCM, sugerindo uma anemia macrocítica hipocrômica no período de 20, 40 e 60 dias. Os coelhos infectados também apresentaram leucopenia e linfopenia, além de uma redução significativa no número de neutrófilos e monócitos, quando comparados com os animais do grupo controle.The present paper was aimed at evaluating hematological changes in rabbits experimentally infected by Trypanosoma evansi. Six male rabbits divided in to two groups were used, being the control group composed of non-infected animals and the test group composed of animals infected by T. evansi. The animals were kept at room temperature and were analyzed during 120 days, by blood collections on days 1, 20, 40, 60 and 120. Hematological changes were observed in the second and third blood collections and the animals with parasitism had a decrease in blood cells, hemoglobin concentration, hematocrit and mean capsular hemoglobin concentration (MCHC and an increase in the mean capsular volume (MCV suggesting a macrocytic hypocrhomic anemia on days 20, 40 and 60. The infected rabbits also presented leucopenia and lymphopenia, as well a significant decrease of neutrophil and monocite counts in comparison to

  12. Efficacy of CMX001 as a Post Exposure Antiviral in New Zealand White Rabbits Infected with Rabbitpox Virus, a Model for Orthopoxvirus Infections of Humans

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    Scott Foster

    2011-01-01

    Full Text Available CMX001, a lipophilic nucleotide analog formed by covalently linking 3-(hexdecyloxypropan-1-ol to cidofovir (CDV, is being developed as a treatment for smallpox. In the absence of human cases of smallpox, new treatments must be tested for efficacy in animal models. Previously, we demonstrated the efficacy of CMX001 in protecting New Zealand White rabbits from mortality following intradermal infection with rabbitpox virus as a model for smallpox, monkeypox and for treatment of adverse reactions to smallpox vaccination. Here we extend these studies by exploring different dosing regimens and performing randomized, blinded, placebo-controlled studies. In addition, because rabbitpox virus can be transmitted via naturally generated aerosols (animal to animal transmission, we report on studies to test the efficacy of CMX001 in protecting rabbits from lethal rabbitpox virus disease when infection occurs by animal to animal transmission. In all cases, CMX001 treatment was initiated at the onset of observable lesions in the ears to model the use of CMX001 as a treatment for symptomatic smallpox. The results demonstrate that CMX001 is an effective treatment for symptomatic rabbitpox virus infection. The rabbitpox model has key similarities to human smallpox including an incubation period, generalized systemic disease, the occurrence of lesions which may be used as a trigger for initiating therapy, and natural animal to animal spread, making it an appropriate model.

  13. [Accuracy of PCR for the detection of bacterial and fungal DNA in the blood and tissue samples of experimentally infected rabbits].

    Science.gov (United States)

    Fouad, Ali Adil; Kalkancı, Ayşe

    2012-10-01

    Direct demonstration of bacterial and/or fungal nucleic acids in the clinical samples of patients with blood stream infections is crucial in terms of rapid diagnosis, early and accurate therapy and patient management. This study was aimed to determine the presence of bacteria and fungi by polymerase chain reaction (PCR) in the clinical samples of experimental sepsis induced animals, to compare the results with culture and to evaluate the efficiency of PCR in the discrimination of bacteremia and fungemia. A total of 12 rabbits experimentally infected with standard strains of Staphylococcus aureus, Escherichia coli, Aspergillus fumigatus and Candida albicans to generate bacteremia (n= 4), fungemia (n= 4) and polymicrobial blood stream infection (n= 4), were included in the study. A total of 63 specimens of which 27 were blood and 36 were tissue (12 spleen, 12 liver, 12 kidney) samples were collected at 24, 48, 72 and 96th hours of infection. Uninfected healthy rabbits (n= 4), colony suspensions of standard bacterial and fungal strains (n= 15) and human blood samples contaminated with standard bacterial and fungal strains (n= 10) were used as controls. Microbial DNAs were searched by using real-time PCR in all the samples, and quantitative cultures were performed simultaneously. Gram-positive and gram-negative PCR protocols were performed for the samples of bacteremic animals, whereas panfungal PCR, Aspergillus and Candida PCR protocols were performed for the samples of animals with fungemia. All of those PCR protocols were applied separately for the samples of polymicrobial blood stream infection cases. Culture positivity was detected in 8 (29.6%) of the blood samples and bacterial and/or fungal DNAs were demonstrated in 20 (74%) of the blood samples by PCR. Microbial DNAs were also detected in 32 (89%) of 36 tissue samples (11 spleen, 11 liver, 10 kidney). Sensitivity rates of culture method to detect bacteremia and fungemia were 30% and 21.7%, respectively, whereas

  14. Dynamic variation of eosinophil count in rabbits infected with Trichinella Spiralis%感染旋毛虫兔血嗜酸性粒细胞计数的动态变化

    Institute of Scientific and Technical Information of China (English)

    李立宏; 陈立峰; 石永威; 张进顺

    2011-01-01

    Objective To observe the dynamic change oi eosinophu count in rabbits infected with Tnchinella spirahs so as to oiler basic data for eosinophil examination in trichinosis. Methods Experimental rabbits were divided into two groups each consisted of 10 rabits. The rabbits in infected group were inoculated by gavage with 10 000 Trichinella spiralis larvae, 10 rabbits in control group. The blood samples were obtained from rabbits in the two group at different time after infection,the eosinophil absolute count was determined. Results The eosinophil absolute count in infected group raised and reached peak on the fourth week after infection and then began to decline therafter. Conclusions The detection of eosinophil examination has assistant values in diagnosis of trichinosis.%目的 观察旋毛虫感染免后血嗜酸性粒细胞的动态变化,为旋毛虫病(Trichinosis)血嗜酸性粒细胞的检测提供基础资料.方法 将实验用兔随机分组:感染组10只,每只喂入10 000条幼虫;对照组10只.在感染后不同时间检测血嗜酸性粒细胞绝对数.结果 感染旋毛虫兔血嗜酸性粒细胞计数升高,于第4周达高峰,随后逐渐降低.结论 嗜酸性粒细胞的检测对于旋毛虫病具有辅助诊断意义.

  15. Ultrastructural Changes in the Intestine of Suckling Rabbits Infected with Cholerogenic and Non-Cholerogenic nonO1/nonO139 Vibrio cholerae Strains.

    Science.gov (United States)

    Monakhova, E V; Fedorenko, G M; Mazrukho, A B; Bardakhch'yan, E A

    2015-09-01

    We performed an electron microscopic study of the small intestine of suckling rabbits infected with cholerogenic and non-cholerogenic strains nonO1/nonO139 Vibrio cholerae. Cholerogenic strain induced mostly hydropic degeneration of the epithelium typical of cholera toxin effect, while non-cholerogenic strain induced the formation of lacunae along the borders of adjacent epithelial cells typical of hemagglutinin/protease effect. In both cases, reduction of microvilli, destruction of intracellular organelles, two types of mitochondrial reaction (condensation and swelling with destruction of cristae), appearance of myelin figures, defects in the capillary walls, and activation of pinocytosis were observed. These data confirm our previous assumption on interchangeability of different pathogenic factors of Vibrio cholerae, including nonO1/nonO139 strains.

  16. Seat belt reminders.

    NARCIS (Netherlands)

    2008-01-01

    Seat belts are an effective way of reducing the number or road deaths and severe road injuries in crashes. Seat belt reminders warn car drivers and passengers if the seat belt is not fastened. This can be done by a visual signal or an acoustic signal or by a combination of the two. Seat belt reminde

  17. Deep sequencing-based identification of pathogen-specific microRNAs in the plasma of rabbits infected with Schistosoma japonicum.

    Science.gov (United States)

    Cheng, Guofeng; Luo, Rong; Hu, Chao; Cao, Jie; Jin, Youxin

    2013-12-01

    Circulating microRNAs (miRNAs) have received considerable attention as a novel class of biomarkers for the diagnosis of cancer and as signalling molecules in mediating intercellular communication. Schistosomes, the causative agents of schistosomiasis, live in the blood vessels of a mammalian host in the adult stage. In the present study, we characterized schistosome-specific small RNA populations in the plasma of rabbits infected with Schistosoma japonicum (S. japonicum) using a deep sequencing method and then identified five schistosome-specific miRNAs, including four known miRNAs (Bantam, miR-3479, miR-10 and miR-3096), and one novel miRNA (miR-0001, miRBase ID: sja-miR-8185). Four of the five schistosome-specific miRNAs were also detected by real-time RT-PCR in the plasma of S. japonicum-infected mice. In addition, our study indicated that schistosome Argonaute 2/3 may be an excretory-secretory (ES) protein. In summary, our findings are expected to provide useful information for further development of novel biomarkers for the diagnosis of schistosomiasis and also for deeper understanding of the mechanism of host-parasite interaction.

  18. Insights into the Staphylococcus aureus-host interface: global changes in host and pathogen gene expression in a rabbit skin infection model.

    Directory of Open Access Journals (Sweden)

    Natalia Malachowa

    Full Text Available Staphylococcus aureus is an important cause of human skin and soft tissue infections (SSTIs globally. Notably, 80% of all SSTIs are caused by S. aureus, of which ∼63% are abscesses and/or cellulitis. Although progress has been made, our knowledge of the host and pathogen factors that contribute to the pathogenesis of SSTIs is incomplete. To provide a more comprehensive view of this process, we monitored changes in the S. aureus transcriptome and selected host proinflammatory molecules during abscess formation and resolution in a rabbit skin infection model. Within the first 24 h, S. aureus transcripts involved in DNA repair, metabolite transport, and metabolism were up-regulated, suggesting an increase in the machinery encoding molecules involved in replication and cell division. There was also increased expression of genes encoding virulence factors, namely secreted toxins and fibronectin and/or fibrinogen-binding proteins. Of the host genes tested, we found that transcripts encoding IL-8, IL1β, oncostatin M-like, CCR1, CXCR1 (IL8RA, CCL4 (MIP-1β and CCL3 (MIP1α-like proteins were among the most highly up-regulated transcripts during S. aureus abscess formation. Our findings provide additional insight into the pathogenesis of S. aureus SSTIs, including a temporal component of the host response. These results serve as a springboard for future studies directed to better understand how/why mild or moderate SSTIs progress to invasive disease.

  19. Belt attachment and system

    Science.gov (United States)

    Schneider, Abraham D.; Davidson, Erick M.

    2016-02-02

    Disclosed herein is a belt assembly including a flexible belt with an improved belt attachment. The belt attachment includes two crossbars spaced along the length of the belt. The crossbars retain bearings that allow predetermined movement in six degrees of freedom. The crossbars are connected by a rigid body that attaches to the bearings. Implements that are attached to the rigid body are simply supported but restrained in pitching rotation.

  20. Genetic Variability of the Resistance for Bacterial Infection in Growing Meat Rabbits%生长肉兔细菌感染抗病力的遗传变异

    Institute of Scientific and Technical Information of China (English)

    张翔宇; 黄邓萍; 谢晓红; 李金良; 雷岷; 杨锐

    2012-01-01

    Bacterial infection is one of the most economically important diseases in preweaned meat rabbits. This study examined the health records of 787 meat rabbits representing four breeds to describe the genetic variability for the resistance to bacterial infection in the growing rabbits from 5 to 10 weeks of age. Incidence of bacterial infection during postweaning periods were analyzed with a threshold sire model. Our results demonstrated that incidence of infection increased steadily with age from a minimum at 5 weeks of age to maximum at 9 weeks of age. The breed and sire effect were significant for incidence of bacterial infection in growing meat rabbits (P<0.05). Flemish Giant had the highest incidence during the postweaning periods (36.87%). 3.42%-8.02% of the phenotypic variation in bacterial infection occurrence was estimated to be due to sire variation. So, there is a genetic variability for the resistance to bacterial infection in meat rabbits- It may be possible to reduce the incidence of bacterial infection using genetic selection.%细菌感染是造成断奶后肉兔重大经济损失的疾病之一.本研究通过对4个品种787只5~10周龄断奶肉兔健康记录的分析,确定细菌感染疾病抗病力的遗传方差.利用父性阈值模型分析断奶后肉兔细菌感染疾病的发病情况.结果表明:细菌感染的发病率从第5周的最小值逐步增加到第9周的最大值.品种和父性效应对生长肉兔细菌感染的发病率影响显著(P<0.05).比利时兔的发病率最高(36 87%),父性方差占到了总表型方差的3.42%~8.02%.因此,肉兔细菌感染疾病的抗病力存在遗传变异,通过遗传选择可以降低细菌感染的发病率.

  1. Lytic HSV-1 infection induces the multifunctional transcription factor Early Growth Response-1 (EGR-1 in rabbit corneal cells

    Directory of Open Access Journals (Sweden)

    McFerrin Harris E

    2011-05-01

    Full Text Available Abstract Background Herpes simplex virus type-1 (HSV-1 infections can cause a number of diseases ranging from simple cold sores to dangerous keratitis and lethal encephalitis. The interaction between virus and host cells, critical for viral replication, is being extensively investigated by many laboratories. In this study, we tested the hypothesis that HSV-1 lytic infection triggers the expression of important multi-functional transcription factor Egr1. The mechanisms of induction are mediated, at least in part, by signaling pathways such as NFκB and CREB. Methods SIRC, VERO, and 293HEK cell lines were infected with HSV-1, and the Egr-1 transcript and protein were detected by RT-PCR and Western blot, respectively. The localization and expression profile of Egr-1 were investigated further by immunofluorescence microscopy analyses. The recruitment of transcription factors to the Egr-1 promoter during infection was studied by chromatin immunoprecipitation (ChIP. Various inhibitors and dominant-negative mutant were used to assess the mechanisms of Egr-1 induction and their effects were addressed by immunofluorescence microscopy. Results Western blot analyses showed that Egr-1 was absent in uninfected cells; however, the protein was detected 24-72 hours post treatment, and the response was directly proportional to the titer of the virus used for infection. Using recombinant HSV-1 expressing EGFP, Egr-1 was detected only in the infected cells. ChIP assays demonstrated that NFкB and cAMP response element binding protein (CREB were recruited to the Egr-1 promoter upon infection. Additional studies showed that inhibitors of NFкB and dominant-negative CREB repressed the Egr-1 induction by HSV-1 infection. Conclusion Collectively, these results demonstrate that Egr-1 is expressed rapidly upon HSV-1 infection and that this novel induction could be due to the NFкB/CREB-mediated transactivation. Egr-1 induction might play a key role in the viral gene

  2. Screening for Anthelminthic Activities from Extracts of Zanthoxylum Zanthoxyloides, Neocarya Macrophylla and Celosia Laxa Against Ascaris Infection in Rabbits

    Directory of Open Access Journals (Sweden)

    B B Barnabas

    2010-12-01

    Full Text Available Summary: Extracts of Celosia laxa, Neocarya macrophylla and Zanthoxylum zanthoxyloides leaves were screened for anthelminthic activities on Ascaris lumbricoides. Celosia laxa appeared to be more potent against A. lumbricoides with rapid recovery of weight while Z. zanthoxyloides has the lowest potency. The effects of these plant extracts on Ascaris lumbricoides suggest that they could serve as an alternative source of anthelminthic agent. Industrial relevance: Infectious diseases pose serious challenges to global health care delivery. Moreoever, in developing countries, the cost of orthodox medication is out of reach of the common man, especially those in rural areas. This is coupled with the adulterated drugs and emergences of drug resistant strains have led to an intensive search for natural products of higher plants, which are biologically active against parasitic infections. Many plants are used locally in traditional medicine to treat parasitic infections. Nigeria offers a rich floral diversity that are used traditionally to treat worm infections. Nigerian medicinal plants (Celosia laxa, Neocarya macrophylla, Zanthoxylum zanthoxyloides used commonly used in Bida, Niger State, Nigeria for the treatment of worm infections were screened for anthelminthic activity. The extracts exhibited varying degree of anthelminthic activities against Ascaris lumbricoides. The extract of C. laxa display potent anthelminthic property. The extract of C. laxa with pronounced activity against A. lumbricoides may contain metabolites that have pharmacologically active principles with promising anthelminthic properties. This indicates the medical potential of C. laxa in the treatment of helminthiasis.

  3. Dermal Wound Transcriptomic Responses to Infection with Pseudomonas aeruginosa versus Klebsiella pneumoniae in a Rabbit Ear Wound Model

    Science.gov (United States)

    2014-05-02

    syndrome toxin 1. Infect lrnrnun 2005, 73:2164 2174. 65. Narasimhan J, Staschke KA, Wek RC: Dimerization is required for activation of eiF2 kinase Gcn2 in...145 154. 67. Papa FR: Endoplasmic reticulum stress, pancreatic beta cell degeneration, and diabetes. Cold Spring Harbor perspectives In medlcile 2012

  4. Novel nitric oxide producing probiotic wound healing patch: preparation and in vivo analysis in a New Zealand white rabbit model of ischaemic and infected wounds.

    Science.gov (United States)

    Jones, Mitchell; Ganopolsky, Jorge G; Labbé, Alain; Gilardino, Mirko; Wahl, Christopher; Martoni, Christopher; Prakash, Satya

    2012-06-01

    The treatment of chronic wounds poses a significant challenge for clinicians and patients alike. Here we report design and preclinical efficacy of a novel nitric oxide gas (gNO)-producing probiotic patch for wound healing. Specifically, a wound healing patch using lactic acid bacteria in an adhesive gas permeable membrane has been designed and investigated for treating ischaemic and infected full-thickness dermal wounds in a New Zealand white rabbit model for ischaemic wound healing. Kaplan-Meier survival curves showed increased wound closure with gNO-producing patch-treated wounds over 21 days of therapy (log-rank P = 0·0225 and Wilcoxon P = 0·0113). Cox proportional hazard regression showed that gNO-producing patch-treated wounds were 2·52 times more likely to close compared with control patches (hazard P = 0·0375, score P = 0·032 and likelihood ratio P = 0·0355), and histological analysis showed improved wound healing in gNO-producing patch-treated animals. This study may provide an effective, safe and less costly alternative for treating chronic wounds.

  5. Rabbit hematology.

    Science.gov (United States)

    Marshall, Kemba L

    2008-09-01

    Using laboratory animal medicine as an established resource, companion animal veterinarians have access to many physiologic and basic science studies that we can now merge with our clinical impressions. By working with reference laboratories, companion animal veterinarians are poised to accelerate our knowledge of the normal rabbit rapidly. The aim of this article is to discuss normal hematopoiesis and infectious and metabolic diseases that specifically target the hemolymphatic system. Additionally, photographic representation of cell types is provided.

  6. The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits

    Science.gov (United States)

    Srivastava, Ruchi; Dervillez, Xavier; Khan, Arif A.; Chentoufi, Aziz A.; Chilukuri, Sravya; Shukr, Nora; Fazli, Yasmin; Ong, Nicolas N.; Afifi, Rasha E.; Osorio, Nelson; Geertsema, Roger; Nesburn, Anthony B.

    2016-01-01

    ABSTRACT Persistent pathogens, such as herpes simplex virus 1 (HSV-1), have evolved a variety of immune evasion strategies to avoid being detected and destroyed by the host's immune system. A dynamic cross talk appears to occur between the HSV-1 latency-associated transcript (LAT), the only viral gene that is abundantly transcribed during latency, and the CD8+ T cells that reside in HSV-1 latently infected human and rabbit trigeminal ganglia (TG). The reactivation phenotype of TG that are latently infected with wild-type HSV-1 or with LAT-rescued mutant (i.e., LAT+ TG) is significantly higher than TG latently infected with LAT-null mutant (i.e., LAT− TG). Whether LAT promotes virus reactivation by selectively shaping a unique repertoire of HSV-specific CD8+ T cells in LAT+ TG is unknown. In the present study, we assessed the frequency, function, and exhaustion status of TG-resident CD8+ T cells specific to 40 epitopes derived from HSV-1 gB, gD, VP11/12, and VP13/14 proteins, in human leukocyte antigen (HLA-A*0201) transgenic rabbits infected ocularly with LAT+ versus LAT– virus. Compared to CD8+ T cells from LAT– TG, CD8+ T cells from LAT+ TG (i) recognized a broader selection of nonoverlapping HSV-1 epitopes, (ii) expressed higher levels of PD-1, TIM-3, and CTLA-4 markers of exhaustion, and (iii) produced less tumor necrosis factor alpha, gamma interferon, and granzyme B. These results suggest a novel immune evasion mechanism by which the HSV-1 LAT may contribute to the shaping of a broader repertoire of exhausted HSV-specific CD8+ T cells in latently infected TG, thus allowing for increased viral reactivation. IMPORTANCE A significantly larger repertoire of dysfunctional (exhausted) HSV-specific CD8+ T cells were found in the TG of HLA transgenic rabbits latently infected with wild-type HSV-1 or with LAT-rescued mutant (i.e., LAT+ TG) than in a more restricted repertoire of functional HSV-specific CD8+ T cells in the TG of HLA transgenic rabbits latently

  7. A novel total knee arthroplasty infection model in rabbits%兔膝关节置换假体感染模型的建立

    Institute of Scientific and Technical Information of China (English)

    郝立波; 邢庆昌; 王岩; 王继芳

    2009-01-01

    目的 制作兔膝关节置换假体感染模型,为人工关节假体感染研究提供依据.方法 成年新西兰大白兔32只,雌雄不限,体重2.5~3.5 k,平均3.0 kg.将采用超高分子量聚乙烯(UHMWPE)制成的兔膝关节且垒骨假体植入兔膝关节.32只动物随机分为四组,关节置换术后1周分别向膝关节内注入无菌生理盐水、1×10~4 CFU、1×10~6 CFU和1×10~8 CFU的ATCC35984葡萄球菌菌液,模拟膝关节置换假体感染.1 周后取材,进行感染率和炎症程度评定、影像学和病理学检查、细菌学评定.结果 接种1×10~6 CFU的细菌足以致所有动物关节感染,超过此剂量则会引起败血症,细菌接种量在0~1×10~8 CFU时感染率和接种细菌量呈正相关(R~2=0.9939).大体观察感染膝关节充满脓液,关节滑膜红肿.激光共聚焦显微镜观察:假体表面黏附大量细菌,并有片状胞外黏质物.病理学检查为急性炎症反应.细菌定量分析发现关节液内细菌量明显高于假体表面和组织内细菌量.结论 制作的兔膝关节置换假体感染模型较好地模拟了临床膝关节感染情况,是一种较为成功的膝关节置换假体感染模型,可用于人工关节假体感染预防、诊断和治疗的研究.%Objective An in vivo animal model was developed to test the efficacy of innovative therapies for the prevention of biomaterial centered infections caused by E. Staphylococcus. Methods Thir-ty-two New Zealand white rabbits were used in this study. After proper anesthesia, a knee prosthesis made by UHMWPE was cemented in the surface of tibia of knee. After 1 week of operation, the operated knee was inoculated with 0, 1×10~4, 1×10~6, and 1×10~8 colony forming units (CFU) of E. Staphylococcus. Animals were sacrificed after 1 week at which time tissues and biomaterial samples were examined for evidence of infec-tion. Joint tissue, implant and joint fluid were tested for the number of bacteria. The joint and implant sam

  8. Efeitos do Foscarnet sobre a infecção pelos herpesvírus bovino tipos 1 e 5 em coelhos Effects of Foscarnet on the infection by bovine herpesviruses 1 and 5 in rabbits

    Directory of Open Access Journals (Sweden)

    Renata Dezengrini

    2010-08-01

    Full Text Available O efeito antiviral do Foscarnet (PFA foi demonstrado anteriormente em células de cultivo infectadas com três herpesvírus bovino (BoHV. No presente estudo, investigaram-se os seus efeitos sobre a infecção e doença causadas pelo BoHV-1 e BoHV-5 em coelhos infectados experimentalmente. Coelhos inoculados com o BoHV-5 pela via intraconjuntival (IC e tratados com o PFA (100mg/kg/dia a partir do dia 1 pós-inoculação (pi apresentaram uma redução nos títulos de vírus excretados entre os dias 2 e 6 pi em comparação com o grupo não-tratado; essa diferença foi significativa no dia 3 pi [F(9,108 = 2,23; PThe activity of Foscarnet (PFA against three bovine herpesviruses (BoHVs was previously demonstrated in cell culture. Herein we evaluated the effects of PFA on the infection and disease by BoHV-1 and BoHV-5 in a rabbit model. Rabbits inoculated with BoHV-5 in the conjunctival sac (IC and treated with PFA (100 mg/kg/day from day 1 to 17 post-inoculation (pi shed less virus between days 2 and 6 pi comparing to untreated controls; this difference was significant at day 3 pi [F(9,108 = 2,23; P<0.03]. The morbidity and mortality rates of rabbits inoculated with BoHV-5 IC or intranasally (IN were also significantly reduced in PFA-treated rabbits (50%; 11/22 comparing to untreated controls (95.4%; 21/22 (P<0.0008. In rabbits inoculated IC with BoHV-1, a reduction in virus shedding was observed in PFA-treated animals between days 1 and 4 pi; 6 and 7 pi. In addition, PFA-treated rabbits presented a longer incubation period and a shorter clinical course comparing to untreated controls (P<0.005 and P<0.04, respectively. The frequency and severity of ocular signs were also reduced in the PFA-treated group. These results demonstrate that PFA is effective against BoHV-1 and BoHV-5 in vivo and open the way towards its use in experimental therapy of herpetic infections in domestic animals.

  9. Persistence of Salmonella on egg conveyor belts is dependent on the belt type but not on the rdar morphotype.

    Science.gov (United States)

    Stocki, S L; Annett, C B; Sibley, C D; McLaws, M; Checkley, S L; Singh, N; Surette, M G; White, A P

    2007-11-01

    Commercial caged layer flocks in Alberta, Canada, are commonly monitored for Salmonella enterica serovar Enteritidis (SE) and S. enterica serovar Typhimurium (ST) by environmental sampling. In one recent case, a SE strain isolated from the egg conveyor belt was a source of persistent infection for the flock. This study was undertaken to examine Salmonella colonization on egg conveyor belts and to determine whether the rdar morphotype, a conserved physiology associated with aggregation and long-term survival, contributed to persistence. Four woven belts constructed of natural or nonnatural fibers and a 1-piece belt made of vinyl were tested with rdar-positive ST and SE strains and a rdar-negative ST DeltaagfD reference strain. The type of egg belt was the most important factor influencing Salmonella colonization and persistence. The vinyl belt, with the least surface area available for colonization, had the fewest Salmonella remaining after washing and disinfection, whereas the hemp-plastic belt, with the greatest surface area, had the most Salmonella remaining. Real-time gene expression indicated that the rdar morphotype was involved in colonizing the egg belt pieces; however, it was not essential for persistence. In addition, rdar-positive and rdar-negative strains were equally similarly to disinfection on the egg belt pieces. The results indicate that Salmonella can persist on a variety of egg belts by mechanisms other than the rdar morphotype, and that using egg conveyer belts with reduced surface area for bacterial colonization can lessen contamination problems.

  10. The European rabbit (Oryctolagus cuniculus), a source of zoonotic cryptosporidiosis.

    Science.gov (United States)

    Robinson, G; Chalmers, R M

    2010-12-01

    Cryptosporidium spp. have been found in the faeces of over 150 mammalian host species, but the risks to public health from wildlife are poorly understood. In summer 2008, the Cryptosporidium sp. rabbit genotype was identified as the aetiological agent in an outbreak of waterborne human cryptosporidiosis. The source was a wild rabbit that had entered a treated water tank. To establish current knowledge about Cryptosporidium spp. infecting lagomorphs, especially the host range and biological characteristics of the rabbit genotype, and the potential risks to public health that rabbits may pose in the transmission of zoonotic cryptosporidiosis, we undertook a literature and data review. The literature returned demonstrates that although the European rabbit (Oryctolagus cuniculus) has been the most widely studied lagomorph, few large scale studies were found. The prevalence of Cryptosporidium spp. in wild rabbit populations in the two large scale studies was 0.9% (95%CI 0.2-5.0) and 0.0% (95%CI 0.0-1.6). Neither study provided age nor sex profiles nor typing of Cryptosporidium isolates. The infecting Cryptosporidium species was confirmed in just four other studies of rabbits, all of which showed the rabbit genotype. Human-infectious Cryptosporidium species including Cryptosporidium parvum have caused experimental infections in rabbits and it is likely that this may also occur naturally. No published studies of the host range and biological features of the Cryptosporidium rabbit genotype were identified, but information was generated on the identification and differentiation of the rabbit genotype at various genetic loci. Both pet and wild rabbits are a potential source of human cryptosporidiosis and as such, good hygiene practices are recommended during and after handling rabbits or exposure to their faeces, or potentially contaminated surfaces. Water supplies should be protected against access by wildlife, including rabbits.

  11. Risk of zoonotic transmission of HEV from rabbits.

    Science.gov (United States)

    Lhomme, Sébastien; Dubois, Martine; Abravanel, Florence; Top, Sokunthea; Bertagnoli, Stéphane; Guerin, Jean-Luc; Izopet, Jacques

    2013-10-01

    Hepatitis E virus strains from rabbits indicate that these mammals may be a reservoir for HEVs that cause infection in humans. Further issues remain to be clarified, including whether the genotype of rabbit HEV differs from human and swine HEV genotype 3 and whether rabbit HEV can infect human and other animals. HEV was found in farmed rabbits in several geographic areas of China, in USA and more recently in France. The prevalence of antibodies against HEV was 36%, 57% and 55% in rabbits from Virginia (USA), Gansu Province and Beijing (China), respectively. HEV RNA was detected in 16.5% of serum samples from farmed rabbits in Virginia, 7.5% in Gansu Province and 7.0% in Beijing. HEV RNA was detected in 7% of bile samples from farmed rabbits and in 23% of liver samples from wild rabbits in France. The full-length genomic sequences analysis indicates that all the rabbit strains belong to the same clade. Nucleotide sequences were 72.2-78.2% identical to HEV genotypes 1-4. Comparison with HEV sequences of human strains circulating in France and reference sequences identified a human strain closely related to rabbit HEV. A 93-nucleotide insertion in the X domain of the ORF1 of the human strain and in all the rabbit HEV strains was found. Moreover, the ability of rabbit HEV to cause cross-species infection in a pig model has recently been demonstrated. Rabbit HEV can replicate efficiently in human cell lines. Collectively, these data support the possibility of zoonotic transmission of HEV from rabbits.

  12. Seat belt restraint system

    Science.gov (United States)

    Garavaglia, A.; Matsuhiro, D.

    1972-01-01

    Shoulder-harness and lap-belt restraint system was designed to be worn by individuals of widely different sizes and to permit normal body motion except under sudden deceleration. System is divided into two basic assemblies, lap belt and torso or shoulder harness. Inertia-activated reels immediately lock when seat experiences sudden deceleration.

  13. The importance of subcutaneous abscess infection by Pasteurella spp. and Staphylococcus aureus as a cause of meat condemnation in slaughtered commercial rabbits

    Directory of Open Access Journals (Sweden)

    A. Ferreira

    2014-12-01

    Full Text Available Subcutaneous abscesses are lesions frequently reported in commercial rabbits. Both at farm and slaughterhouse level, these lesions are responsible for economic losses and a potential decrease in meat quality. The present study was devised to identify the main causes of meat condemnation in slaughtered commercial rabbits and assess the importance of abscess lesions in this domain. For these purposes, 281423 rabbits were evaluated during meat inspection at the slaughterhouse. The results achieved showed that subcutaneous abscesses were the major cause of condemnation, being responsible for the rejection of 1355 (0.48% rabbit carcasses. The main affected area was the hind limbs (31.37%, followed by the cervical area (23.10%. Microbiological analyses of 27 abscess samples indicated Pasteurella spp. as the bacteria mostly isolated (59.3%, followed by Staphylococcus aureus (25.9%. These results enable us to advise the industry on the significance of abscesses as an important cause of economic losses, due to meat condemnation during post mortem inspection, and highlight the importance of implementing monitoring plans as a way to control this pathological problem.

  14. Detection of the new emerging rabbit haemorrhagic disease type 2 virus (RHDV2) in Sicily from rabbit (Oryctolagus cuniculus) and Italian hare (Lepus corsicanus).

    Science.gov (United States)

    Camarda, A; Pugliese, N; Cavadini, P; Circella, E; Capucci, L; Caroli, A; Legretto, M; Mallia, E; Lavazza, A

    2014-12-01

    Rabbit haemorrhagic disease virus (RHDV), a member of the genus Lagovirus, causes rabbit haemorrhagic disease (RHD), a fatal hepatitis of rabbits, not previously reported in hares. Recently, a new RHDV-related virus emerged, called RHDV2. This lagovirus can cause RHD in rabbits and disease and mortality in Lepus capensis (Cape hare). Here we describe a case of RHDV2 infection in another hare species, Lepus corsicanus, during a concurrent RHD outbreak in a group of wild rabbits. The same RHDV2 strain infected rabbits and a hare, also causing a RHD-like syndrome in the latter. Our findings confirmed the capability of RHDV2 to infect hosts other than rabbits and improve the knowledge about the epidemiology and the host range of this new lagovirus.

  15. The Rabbit Stream Cipher

    DEFF Research Database (Denmark)

    Boesgaard, Martin; Vesterager, Mette; Zenner, Erik

    2008-01-01

    The stream cipher Rabbit was first presented at FSE 2003, and no attacks against it have been published until now. With a measured encryption/decryption speed of 3.7 clock cycles per byte on a Pentium III processor, Rabbit does also provide very high performance. This paper gives a concise...... description of the Rabbit design and some of the cryptanalytic results available....

  16. The Complement-Fixation Test in Hepatic Coccidiosis of Rabbits

    Science.gov (United States)

    Rose, M. Elaine

    1961-01-01

    Antibodies to Eimeria stiedae were measured in rabbit serum by complement fixation. The titre rose to a maximum at about the 22nd day after infection, remained at this level for about 20 days and then declined. Antibodies were still detectable up to 160 days after infection. Evidence of past or present slight E. stiedae infection was found in clinically normal rabbits whose sera fixed complement with E. stiedae antigens. Challenge of rabbits which had recovered from a near-fatal infection had no effect upon the complement fixation titres of their sera. The serum of a rabbit which had been injected with alum-precipitated antigen fixed complement with E. stiedae antigens. However, the animal was still susceptible to a superimposed oral infection which had the effect of further increasing the serum titre. PMID:14493840

  17. Rabbit haemorrhagic disease: advantages of cELISA in assessing immunity in wild rabbits (Oryctolagus cuniculus).

    Science.gov (United States)

    Zheng, Tao; Parkes, John P

    2011-12-15

    Rabbit haemorrhagic disease (RHD) is an acute fatal disease of domestic and wild European rabbits (Oryctolagus cuniculus) caused by RHD virus (RHDV). Accurate assessment of immunity is of great importance for the conservation and control of wild rabbits. We evaluated a competitive ELISA (cELISA) against isotype ELISAs for assessing the protective immunity against the disease by challenging 50 wild-caught rabbits with a lethal dose of RHDV. Death or survival to the challenge was used as a criterion to determine the performance characteristics of the assay for the assessment of immunity in rabbits. At 1:10 dilution, a serum exhibiting ≥ 25% inhibition (1:10(25)) was regarded as the presence of RHDV-specific antibodies. Eleven of 16 (68.8%) rabbits with antibodies at 1:10(25) (rabbit calicivirus, which interfered with isotype ELISAs, had little impact on the specificity of the cELISA for the diagnosis of RHDV infection. The presence of RHDV-specific antibody at 1:10(50) by the cELISA is a reliable indicator for the protective immunity. In contrast to isotype ELISAs, the cELISA is a valuable specific tool for monitoring the herd immunity to RHD for the conservation and management of wild rabbits in the field.

  18. Establishment of prosthesis-related infection model after total knee arthroplasty in rabbits%兔膝关节置换术后感染模型的建立

    Institute of Scientific and Technical Information of China (English)

    王志酬; 曹力; 龚时国; 曾赘; 刘阳

    2010-01-01

    目的 建立兔膝关节置换(TKA)术后感染模型.方法 设计制作兔膝关节假体,新西兰兔48只行右膝TKA,4周后随机分为4组:对照组膝关节腔内接种无菌0.45%NaCl溶液,实验A、B、C组分别接种5×10~3、5×10~5、5×10~7CFU耐甲氧西林金葡菌(MRSA);监测血沉(ESR)、C-反应蛋白(CRP),4周后处死,取材行细菌培养和血培养.结果 感染率:对照组0(0/12);实验A组58.3%(7/12);B组100.0%(12/12);C组100.0%(12/12).血培养阳性率:对照组0(0/12);实验A组0(0/12);B组0(0/12);C组41.7%(5/12).实验组CRP、ESR显著升高,分别于第3、7天达峰值并维持在较高水平.结论 兔TKA术后4周接种5×10~5CFU的MRSA可成功建模.%Objective An animal model was developed to test the efficacy of innovative therapies for the prevention of biomaterial centered infections in knee caused by methicillin-resistant Staphylococcus anreus (MRSA) bacteria. Methods Prostheses of rabbit knees were successfully designed and consisted of two components: the femoral components were manufactured from Co-Cr-Mo alloy, and the tibial tray was made of high molecular weight polyethylene. They were implanted and fixed with bone cement in 48 tight knees of New Zealand White rabbits after proper anesthesia. After 4 weeks, the rabbits were randomly divided into 4 groups, including control group, experimental groups A, B and C. Each right knee was respectively inoculated with 0.5×10~3, 5×10~5, 5×10~7colony forming units (CFU) MRSA. The levels of serum C-reaction protein (CRP) and erythrocyte sedimentation rate (ESR) were monitored. Animals were sacrificed after 4 weeks. Joint aspirate, tissues, biomaterial and blood samples were examined for evidence of infection. Results None of the knees in the control group was infected after injection of 0.45% saline. Seven out of 12 knees developed a biomaterial centered infection in group A when 5×10~3 CFU MRSA were introduced. All knees developed a biomaterial centered infection in group

  19. Neuropatogênese experimental da infecção pelo herpesvírus bovino tipo 5 em coelhos Experimental neuropathogenesis of bovine herpesvirus 5 infection in rabbits

    Directory of Open Access Journals (Sweden)

    Eduardo Furtado Flores

    2009-01-01

    estudar diversos aspectos da infecção pelo BoHV-5.Several aspects of the biology of bovine herpesvirus 5 (BoHV-5 have been studied in rabbits, which develop acute infection and neurological disease upon experimental inoculation. The acute infection is followed by the establishment of latent infection, which can be naturally or artificially reactivated. The first experiments in rabbits established a protocol for virus inoculation and monitoring the infection, and characterized the main virological, clinical and pathological aspects of the acute infection. The pathogenesis of acute infection, from the initial viral replication at site of inoculation, pathways and kinetics of viral transport to the brain, distribution and virus replication in the central nervous system (CNS, cellular and tissue tropism, clinical signs and CNS pathology have been extensively studied using this animal model. Subsequently, several biological and molecular aspects of latent BoHV-5 infection have also been elucidated upon inoculation of rabbits. Rabbits have also been used to investigate the phenotype (neuroinvasiveness, neurogrowth of field isolates and recombinant vaccine candidates, protection by passive immunity, vaccine protection, the efficacy of anti-viral drugs and support therapies for neurological disease. This animal model was also used to investigate the origin and distribution of electric impulses involved in seizures - a hallmark of BoHV-5 induced neurological infection - and also to test the efficacy of anti-convulsivants. In spite of the possible differences between rabbits and cattle - the natural host of the virus - the observations taken from this experimental model have greatly contributed to the knowledge of the biology of BoHV-5 infection. The present article presents a review of the main published and unpublished results and observations by our group, comprising more than a decade of studies on the pathogenesis of BoHV-5 infection in the rabbit model.

  20. Confirmation and phylogenetic analysis of rabbit hemorrhagic disease virus in free-living rabbits from the Netherlands

    NARCIS (Netherlands)

    van de Bildt, M. W. G.; van Bolhuis, G. H.; van Zijderveld, F.; van Riel, D.; Drees, J. M.; Osterhaus, A. D. M. E.; Kuiken, T.

    2006-01-01

    The number of free-living European rabbits (Oryctolagus cuniculus) in the Netherlands has declined dramatically in recent years. Although rabbit hemorrhagic disease virus (RHDV) infection has been implicated as a possible cause of this decline, the definitive diagnosis has not been reported. We exam

  1. Pathological Studies on the Small Intestine of Wild Rabbit Fish (Siganus Rivulatus) Infected by Helminthes Parasite (Procamallanus Sp) in Red Sea Coast Area, Sudan

    OpenAIRE

    Salah Eldeen Y.M.H; Omer F. Idris; Murwan K. Sabahelkhier

    2012-01-01

    Wild rabbit fish (Siganus rivulatus) Forsskål (Teleostei, Siganidae) herbivores fish were collected from two sites (Dongnab and Swakin) on Sudanese Red Sea Coast during February 2010 - January 2011 and then examine for helminthes parasites. Microscopic anatomy of intestine with worms and without worms compared to each other. Helminth parasites belong to Procamallanus sp. The abundance of lymphocytes cell, eosinphils, red blood cells and goblet cells in parasitized intestine was significantly ...

  2. Variant rabbit hemorrhagic disease virus in young rabbits, Spain.

    Science.gov (United States)

    Dalton, Kevin P; Nicieza, Inés; Balseiro, Ana; Muguerza, María A; Rosell, Joan M; Casais, Rosa; Álvarez, Ángel L; Parra, Francisco

    2012-12-01

    Outbreaks of rabbit hemorrhagic disease have occurred recently in young rabbits on farms on the Iberian Peninsula where rabbits were previously vaccinated. Investigation identified a rabbit hemorrhagic disease virus variant genetically related to apathogenic rabbit caliciviruses. Improved antivirus strategies are needed to slow the spread of this pathogen.

  3. Serological Survey for RHD Antibodies in Rabbits from Two Types of Rabbit Breeding Farms.

    Science.gov (United States)

    Fitzner, A; Niedbalski, W

    2016-09-01

    Seroprevalence studies of RHDV antibodies in domestic rabbits were conducted between 2008-2014. A total of 12,169 sera from the provinces of central, southern and south-east Poland, including 7,570 samples collected from mixed-breed rabbits reared in smallholder farms and nearly 4,600 sera taken mainly from unvaccinated rabbits kept in industrial farms, were examined using ELISA tests. Additionally, cross-reactivity of selected tested and control archival sera using both classic RHDV and RHDVa antigens was determined by HI assay. The overall seroprevalence was 13.3%. In rabbits with unkown history of immunisation or RHD infection which came from small farms, RHDV antibodies were detected in 6.1% ranging between 1.0% to 17.2% of animals. In rabbits of the same group, but with a declared vaccination status, or confirmed exposure to an infectious virus, or coming from exposed females, the seroprevalence ranged from 83% to 100%. Among unvaccinated meat rabbits aged 71 to 90 days from industrial farms, low (1.85%, 4.17%, 11%), medium (34%, 54%) or high rates (98.7%) of seropositivity were detected. The seroconversion recorded in adult vaccinated females from industrial farms was 70% and 95%. Generally, the antibody levels examined by ELISAs and HI were comparable. However, a number of sera from the rabbits from small farms, as well as archival sera, showed clear differences. Several-fold differences in antibody titers, evidenced mainly in the postoutbreak sera, indictaed the contact of animals with RHDVa antigen. The overall results of the survey revealed a great proportion of seronegative rabbits potentially highly susceptible to RHD infection. In combination with the emergence of a novel pathogenic RHD virus type (RHDV2), it poses a severe risk of a next wave of fatal disease cases spreading in the native population of domestic rabbits, especially in farms with a traditional system of husbandry.

  4. Rabbit hepatitis E virus is an opportunistic pathogen in specific-pathogen-free rabbits with the capability of cross-species transmission.

    Science.gov (United States)

    Liu, Baoyuan; Sun, Yani; Du, Taofeng; Chen, Yiyang; Wang, Xinjie; Huang, Baicheng; Li, Huixia; Nan, Yuchen; Xiao, Shuqi; Zhang, Gaiping; Hiscox, Julian A; Zhou, En-Min; Zhao, Qin

    2017-03-01

    Hepatitis E virus (HEV) has been detected in rabbits, a recently identified natural reservoir. In this study, anti-HEV antibodies and viral RNA were detected in rabbits sourced from a specific-pathogen-free (SPF) rabbit vendor in Shaanxi Province, China. BLAST results of partial HEV ORF2 genes cloned here indicated that two viral strains circulated in the rabbits. Sequence determination of the complete genome (7302bp) of one strain and a partial ORF1 gene (1537bp) of the other strain showed that they shared 90% identity with one another and 78%-94% identity with other known rabbit HEVs. In addition, inoculation with rabbit HEV from SPF rabbits studied here resulted in infection of SPF pigs; this cross-species transmission was evidenced by seroconversion, viremia and faecal virus shedding. These results suggest that to prevent spread of this zoonotic pathogen, rabbits should be tested routinely for HEV RNA in SPF vendor facilities.

  5. THERAPEUTIC MANAGEMENT OF SARCOPTIC MANGE IN RABBIT WITH IVERMECTIN

    Directory of Open Access Journals (Sweden)

    Joyjit Mitra

    2014-06-01

    Full Text Available Sarcoptic mange infected non-descriptive rabbits were successfully treated with Ivermectin @ 400 µg / kg body weight sub-cutaneously once weekly for 4 weeks resulted complete recovery within a month in Kalyani area, West Bengal, India.

  6. Dynamic characteristics of conveyor belts

    Institute of Scientific and Technical Information of China (English)

    HOU You-fu; MENG Qing-rui

    2008-01-01

    The dynamic characteristics of a belt conveyor are determined to a large extent by the properties of the belt. This paper describes experiments designed to establish the dynamic properties of belting material. The dynamic elastic modulus, viscous damping and theological constants of the belt were measured. Several properties were studied as a function of the tensile loading on the belt. These included longitudinal vibration, the natural vibration frequency in the transverse direction and the response to an impulse excitation. Vibration response was observed under several different excitation frequencies. Most of these properties have not been tested previously under conditions appropriate for the ISO/DP9856 standard. Two types of belt were tested, a steel reinforced belt and a fabric reinforced belt. The test equipment was built to provide data appropriate for designing belt conveyors. It was observed that the stress wave propagation speed increased with tensile load and that tensile load was the main factor influencing longitudinal vibrations.

  7. Immunization with a Recombinant, Pseudomonas fluorescens-Expressed, Mutant Form of Bacillus anthracis-Derived Protective Antigen Protects Rabbits from Anthrax Infection.

    Directory of Open Access Journals (Sweden)

    Matthew D Reed

    Full Text Available Protective antigen (PA, one of the components of the anthrax toxin, is the major component of human anthrax vaccine (Biothrax. Human anthrax vaccines approved in the United States and Europe consist of an alum-adsorbed or precipitated (respectively supernatant material derived from cultures of toxigenic, non-encapsulated strains of Bacillus anthracis. Approved vaccination schedules in humans with either of these vaccines requires several booster shots and occasionally causes adverse injection site reactions. Mutant derivatives of the protective antigen that will not form the anthrax toxins have been described. We have cloned and expressed both mutant (PA SNKE167-ΔFF-315-E308D and native PA molecules recombinantly and purified them. In this study, both the mutant and native PA molecules, formulated with alum (Alhydrogel, elicited high titers of anthrax toxin neutralizing anti-PA antibodies in New Zealand White rabbits. Both mutant and native PA vaccine preparations protected rabbits from lethal, aerosolized, B. anthracis spore challenge subsequent to two immunizations at doses of less than 1 μg.

  8. Immunization with a Recombinant, Pseudomonas fluorescens-Expressed, Mutant Form of Bacillus anthracis-Derived Protective Antigen Protects Rabbits from Anthrax Infection.

    Science.gov (United States)

    Reed, Matthew D; Wilder, Julie A; Mega, William M; Hutt, Julie A; Kuehl, Philip J; Valderas, Michelle W; Chew, Lawrence L; Liang, Bertrand C; Squires, Charles H

    2015-01-01

    Protective antigen (PA), one of the components of the anthrax toxin, is the major component of human anthrax vaccine (Biothrax). Human anthrax vaccines approved in the United States and Europe consist of an alum-adsorbed or precipitated (respectively) supernatant material derived from cultures of toxigenic, non-encapsulated strains of Bacillus anthracis. Approved vaccination schedules in humans with either of these vaccines requires several booster shots and occasionally causes adverse injection site reactions. Mutant derivatives of the protective antigen that will not form the anthrax toxins have been described. We have cloned and expressed both mutant (PA SNKE167-ΔFF-315-E308D) and native PA molecules recombinantly and purified them. In this study, both the mutant and native PA molecules, formulated with alum (Alhydrogel), elicited high titers of anthrax toxin neutralizing anti-PA antibodies in New Zealand White rabbits. Both mutant and native PA vaccine preparations protected rabbits from lethal, aerosolized, B. anthracis spore challenge subsequent to two immunizations at doses of less than 1 μg.

  9. Infections

    Science.gov (United States)

    ... Infections Adenovirus Bronchiolitis Campylobacter Infections Cat Scratch Disease Cellulitis Chickenpox Chlamydia Cold Sores Common Cold Coxsackievirus Infections Croup Cytomegalovirus (CMV) Dengue Fever Diphtheria E. Coli ...

  10. Extraction of IgG from serum of rabbit infected with Schistosoma japonicum by saturated ammonium sulphate%硫酸铵法提取血吸虫感染兔血清IgG初探

    Institute of Scientific and Technical Information of China (English)

    兰炜明; 姜唯声; 谢曙英; 徐银; 戴坤教; 高晓晖; 曾小军

    2016-01-01

    Objective To explore the feasibility of preparing the schistosomiasis positive reference product by the method of ammonium sulfate precipitation to extract the IgG from the infected rabbits ’ serum of Schistosoma japonicum. Methods We used the saturated ammonium sulfate solution to precipitate and separate the IgG from the infected rabbits ’ serum. Compared the titer of infected rabbits’ serum with the separated IgG,and compared unfreeze-drying titer of IgG with the freeze-drying by IHA,also studied the stability of freeze-drying IgG after we dissolve with water. Results The titer of the infected rabbits’ serum was 1:320 used to extract IgG by the method of ammonium sulfate precipitation,we dialysised,concentrated the liquid of the extracted IgG to the original volume,the extracted IgG titer was 1:320. the negative rabbits’ serum and sucrose as the protein protective agent added to the extracted IgG to freeze-drying,the extracted IgG titer of the unfreeze-drying and the freeze-drying were 1:160. The titer of the freeze-drying IgG dissolved in water changed with the time,the 0th,3rd,6th and 8th titer is 1:160,1:80,1:40 and 1:40 respectively. Conclusion All of the process of the ammonium sulfate precipitation method to extract the IgG and the freeze-dry-ing can not chang the titer of the antibody,but the process adding the protein protective agent to the extracted IgG reduced the titer,the titer of freeze-drying IgG dissolve with water falled after the 3rd. The ammonium sulfate precipitation method is suitable for extracting the IgG from the infected rabbit serum.%目的:探索硫酸铵法提取血吸虫感染兔血清IgG用于制备阳性参考品的可行性。方法采用饱和硫酸铵溶液对感染兔血清中IgG进行沉淀分离,IHA试剂测定分离前后、冻干前后的抗体滴度以及冻干IgG复溶后的稳定性。结果滴度为1:320的血吸虫感染兔血清经硫酸铵沉淀法提取IgG,经透析、浓缩至

  11. Seroprevalence of Encephalitozoon cuniculi in Humans and Rabbits in China.

    Directory of Open Access Journals (Sweden)

    Yaoqian Pan

    2015-06-01

    Full Text Available Encephalitozoon cuniculi is a microsporidian parasite commonly found in rabbits that can infect humans, causing encephalitozoonosis. Our objective in this study was to evaluate the seroprevalence of this parasite in rabbits and humans in China.Overall, 300 serum samples each from clinically healthy rabbit and human were collected from three regions of China (Sichuan Province, Chongqing Municipality and Jilin Province from January to September 2013 and tested for anti-E. Cuniculi antibodies using an ELISA.An overall seroprevalence of E. cuniculi was recorded as 56/300 (18.76% and 29/300 (9.76% in rabbit and human sera, respectively. The seropositivity of rabbit samples collected from Jilin province was 41%, which was significantly higher (P<0.01 than Sichuan Province (9% and Chongqing Municipality (6%. Three breeds of rabbit were used in the present study and antibody detection in Rex Rabbit was significantly (P<0.01 higher than Japanese White and New Zealand Rabbit. In human, Jilin province was more prevalent (18% followed by Sichuan Province (6% and Chongqing Municipality (5%.The E. cuniculi was present and widespread among healthy rabbits and humans in China.

  12. 嗜麦芽窄食单胞菌家兔感染动物模型体内药物敏感性监测%Establishment on rabbit model of Stenotrophomonas maltophilia infection and antimicrobial sensitivity monitoring in vivo

    Institute of Scientific and Technical Information of China (English)

    陈秉宇; 吕火祥; 俞赛; 胡庆丰; 沈蓓琼

    2011-01-01

    Objective:To build the rabbits infection model of the Stenotrophomonas maltophilia, then with PiperacillinTazobactam, Ticarcillin - clavulanate, Cefepime cure to monit drug sensitivity in vivo, to pick out the best drug sensitivity test in vitro to show curative effect. Methods: Rabbits were divided into three treatment groups( A, B and C), 19 injected with 0.5 ml Stenotrophomonas maltophilia in every group as the density is l08 cfu · ml-1, one injected with 0. 5 ml NS in every group as the negative control.After inoculation, the rabbits 'anus temperature and leucocyte and Endotoxin (Lap) were determined every 24 hours,and at 48 h、72 h、96 h、12 h、144 h, 1 ml of 2 g/ml Piperacillin - Tazobactam was injected in A group for the rabbits injected with 0. 5 ml Stenotrophomonas maltophilia. In the same way, for B group with 1 ml of 0.6 g/ml Ticarcillin - clavulanate,for C group with lmL of 0.1 g/ml Cefepime, then use paper agar diffusion method ( K - B mdthod) and MIC method and VITEK method for the 57 strains in vitro drug sentivity test. Results: Rabbit models of Stenotrophomonas maltophilia infection were successfully established,and test results consistent with animal modles of course of treatment rate in the group A were 83.3% in MIC method,55.6% in KB method and 77.8% in VITEK method. In the group B were 44.4% in K - B method, 77.8% both in MIC method and in VITEK method. In the group C were 66.7% in K - B method, 88.9% in MIC method and 77.8% in VITEK method. Conclusion: Body temperature and Endotoxin (Lap) to be the most effective price indicators of infection was determined, in the detection method has been used,MIC method from the animal infection and drug sensitivity in animals is most closely monitored, and there is big difference between rabbits in vitro antimicrobial susceptibility and antimicrobial sensitivity.%目的:建立嗜麦芽窄食单胞菌感染的家兔细菌感染模型,观察抗菌药物在家兔体内的药物敏感性,

  13. Radiation belts of jupiter.

    Science.gov (United States)

    Stansberry, K G; White, R S

    1973-12-07

    Predictions of Jupiter's electron and proton radiation belts are based mainly on decimeter observations of 1966 and 1968. Extensive calculations modeling radial diffusion of particles inward from the solar wind and electron synchrotron radiation are used to relate the predictions and observations.

  14. Replication and Evaluation of A Model of Pulmonary Infection of Pseudomonas aeruginasa in Rabbit%铜绿假单胞菌家兔肺部感染模型的建立与评价

    Institute of Scientific and Technical Information of China (English)

    李斌; 朱冬青; 刘洪超; 于红; 刘士远

    2012-01-01

    目的:建立铜绿假单胞菌(PA,又名绿脓杆菌)肺部感染动物模型,并从病理学及细菌学、影像学等方面进行评价,研究铜绿假单胞茵对肺组织的致病作用.方法:24只健康清洁级新西兰大白兔随机分成2组,实验组采用经皮气管穿刺法,隔日注入铜绿假单胞菌反复感染家免,对照组施以生理盐水.接种后隔日一次行胸部CT扫描.比较二组家兔的体温、血象及肺组织匀浆菌落计数;观察病理组织改变及胸部CT影像学变化.结果:注茵组家兔白细胞总数显著升高,肺组织匀浆菌落计数显著高于对照组.CT表现为双侧多发斑片状模糊影,部分可见实变及脓肿灶.大体标本可见家兔肺部结节、脓肿及出血灶,病理学镜下观察发现注茵组家兔肺组织内多个脓肿灶形成,大量中性粒细胞浸润,肺泡腔内大量渗出,部分出血,肺泡壁充血及肺水肿.随着注茵次数增多及时间推移,逐渐出现如肺泡间隔增厚、肉芽肿形成、实变等慢性肺部炎症的病理表现.结论:使用经皮气道穿刺法接种铜绿假单胞茵至新西兰兔肺部,可成功建立兔铜绿假单胞茵肺部感染模型.%Objective: To establish an animal model of lung infection of Pseudomonas aeruginosa (PA) and evaluate its pathology and bacteriology, imaging and other aspects, for investigating the effect of Pseudomonas aeruginosa on lung infection of PA. Methods: 24 healthy, clean New Zealand rabbits were randomly divided into two groups: experiment group were treated with injection of Pseudomonas aeruginosa suspension by means of percutaneous tracheal puncture every other day; rabbits in control group were subjected to sterile saline. Chest CT scan was performed after inoculation and the bacteria number in the lung was determined. Pathological and radiological changes were compared between these two groups. Results: The number of white blood cells (WBC) and bacteria detected from experiment group were

  15. Radiation Belt Dynamics

    Science.gov (United States)

    2015-12-27

    document for any purpose other than Government procurement does not in any way obligate the U.S. Government. The fact that the Government...release; distribution is unlimited. REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 Public reporting burden for this collection of information...Radiation Belt Modeling,” co-lead by J. Albert (AFRL), W. Li (UCLA), S. Morley ( LANL ), and W. Tu (UWV). Figure 6. Precipitating Energy Flux, Simulated

  16. Kuiper Belt Occultation Predictions

    CERN Document Server

    Fraser, Wesley C; Trujillo, Chad; Stephens, Andrew W; Kavelaars, JJ; Brown, Michael E; Bianco, Federica B; Boyle, Richard P; Brucker, Melissa J; Hetherington, Nathan; Joner, Michael; Keel, William C; Langill, Phil P; Lister, Tim; McMillan, Russet J; Young, Leslie

    2013-01-01

    Here we present observations of 7 large Kuiper Belt Objects. From these observations, we extract a point source catalog with $\\sim0.01"$ precision, and astrometry of our target Kuiper Belt Objects with $0.04-0.08"$ precision within that catalog. We have developed a new technique to predict the future occurrence of stellar occultations by Kuiper Belt Objects. The technique makes use of a maximum likelihood approach which determines the best-fit adjustment to cataloged orbital elements of an object. Using simulations of a theoretical object, we discuss the merits and weaknesses of this technique compared to the commonly adopted ephemeris offset approach. We demonstrate that both methods suffer from separate weaknesses, and thus, together provide a fair assessment of the true uncertainty in a particular prediction. We present occultation predictions made by both methods for the 7 tracked objects, with dates as late as 2015. Finally, we discuss observations of three separate close passages of Quaoar to field star...

  17. The potential role of wild rabbits Oryctolagus cuniculus in the epidemiology of paratuberculosis in domestic ruminants.

    Science.gov (United States)

    Daniels, M J; Henderson, D; Greig, A; Stevenson, K; Sharp, J M; Hutchings, M R

    2003-06-01

    Mycobacterium avium subspecies paratuberculosis, the organism responsible for paratuberculosis in cattle and sheep has been found in wild rabbits (Oryctolagus cuniculus) in the east of Scotland. Few studies have investigated either the level of faecal contamination by rabbits on farms, or the potential infectivity of rabbit excreta. The rate of rabbit faecal contamination deposited and the numbers encountered were estimated for 21 fields on 4 farms with a paratuberculosis problem. 7357 +/- 2571 S.E.M. rabbit faecal pellets were deposited per hectare per day and up to 81,000 pellets/ha ('standing crop') were encountered in October/November 1998. Where access to rabbits was restricted, the standing crop of faeces encountered fell to 22,000 pellets/ha. The prevalence of infection with M. a. paratuberculosis was assessed for 83 rabbits from the four farms. M. a. paratuberculosis was isolated from rabbits on all farms with an overall prevalence of 17%. Out of 17 rabbits from which urine was available, M. a. paratuberculosis was isolated from two--the first reported isolation from urine in wild rabbits. The mean number of colony-forming units per gram of infected rabbit faeces was 7.6 x 10(5) +/- 5.2 x 10(5). A relative estimate of the input of M. a. paratuberculosis onto pasture, at the stocking levels found on the four farms, showed that sheep and cattle potentially contributed 4 and 125 times more organisms/ha per day respectively than rabbits. However, rabbits could still contribute millions of M. a. paratuberculosis organisms per ha per day. Existing rabbit control measures on farms may be inadequate in reducing the risk of transmission to livestock.

  18. Zoonoses of rabbits and rodents.

    Science.gov (United States)

    Hill, William Allen; Brown, Julie Paige

    2011-09-01

    Millions of households in the US own rabbits or rodents, including hamsters, guinea pigs, and gerbils. Activities such as hunting and camping also involve human interactions with wild rabbits and rodents. In many environments, feral rabbits and rodents live in close proximity to humans, domesticated animals, and other wildlife. Education of rodent and rabbit owners and individuals with occupational or recreational exposures to these species is paramount to reduce the prevalence of zoonoses associated with rabbit and rodent exposure.

  19. 洋葱伯克霍尔德菌感染家兔抗感染疗效与体外药物敏感性的比较%Comparison of the anti-infective drug sensitivity in rabbit model of Burkholderia cepacia infection and the effect of antimicrobial sensitivity monitoring in vitro

    Institute of Scientific and Technical Information of China (English)

    吕火祥; 胡庆丰; 沈蓓琼; 杨广宇; 俞赛

    2011-01-01

    目的 使用无CLSI完整解释标准的药物,哌拉西林/他唑巴坦、替卡西林/棒酸、头孢吡肟治疗洋葱伯克霍尔德菌感染的家兔模型,观察比较何种体外药物敏感试验更能准确反应临床疗效.方法 57株临床分离的洋葱伯克霍尔德菌,感染57只家兔腹腔.将感染家兔分成3组(A、B、C组),每组19只;分别在48、72、96、120和144 h注射药物:A组每只家兔注射1 mL浓度为2 g/mL哌拉西林/他唑巴坦;B组每只家兔注射1 mL浓度为0.1 g/mL头孢吡肟;C组每只家兔注射1 mL浓度为0.6 g/mL替卡西林/棒酸.比较肉汤稀释法(MIC法)、K-B法及VITEK法检测的药物敏感性结果与体内药物治疗的疗效关系.结果 哌拉西林/他唑巴坦治疗组(A组),K-B法检测结果与家兔感染模型治疗过程相符率为72.2%,MIC法66.7%,VITEK法为66.7%.替卡西林/棒酸治疗组(B组),MIC法83.3%,VITEK法为77.8%,K-B法检测结果与家兔感染模型治疗过程相符率为50.0%.头孢吡肟治疗组(C组),MIC法88.9%,VITEK法为77.8%,K-B法检测结果与家兔感染模型治疗过程相符率为72.2%.结论 在3种药物敏感性检测法中,哌拉西林/他唑巴坦以K-B法检测结果与感染家兔的抗感染疗效最为接近;替卡西林/棒酸及头孢吡肟均以MIC法与感染家兔的抗感染疗效最为接近;针对洋葱伯克霍尔德菌的感染建议临床使用不同的方法检测不同的药物体外敏感性.%Objective To treat rabbit models of Burkholderia cepacia infection by using drugs without complete CLSI interpretive standard, including piperacillin/tazobactam, ticarcillin/clavulanic acid and cefepime, and compare which drug sensitivity test in vitro can more accurately reflect the clinical efficacy. Methods 57 clinical isolates of Burkholderia cepacia were used to infect 57 rabbits intraperitoneally. The infected rabbits were divided into 3 groups ( A, B and C), 19 in each group. Then, at48, 72, 96, 12 and 144 h, respectively, each

  20. RabbitMQ essentials

    CERN Document Server

    Dossot, David

    2014-01-01

    This book is a quick and concise introduction to RabbitMQ. Follow the unique case study of Clever Coney Media as they progressively discover how to fully utilize RabbitMQ, containing clever examples and detailed explanations.Whether you are someone who develops enterprise messaging products professionally or a hobbyist who is already familiar with open source Message Queuing software and you are looking for a new challenge, then this is the book for you. Although you should be familiar with Java, Ruby, and Python to get the most out of the examples, RabbitMQ Essentials will give you the push y

  1. Rabbit orthopedic surgery.

    Science.gov (United States)

    Rich, Gregory A

    2002-01-01

    Orthopedic surgery in rabbits poses several unique parameters for the veterinary surgeon. It is imperative for the veterinarian to be knowledgeable about the anatomic features of the surgical repair site and to become familiar with a rabbit's pain and discomfort often associated with orthopedic injuries. Handling the perioperative and postoperative pain and potential GI disturbances are crucial for a successful outcome of the surgical case. This article is designed to help the veterinary surgeon prepare for the orthopedic surgical procedure and the peripheral physiologic needs of the rabbit from presentation through recovery.

  2. The Alice - "Follow the White Rabbit" - parasites of farm rabbits based on coproscopy.

    Science.gov (United States)

    Kornaś, Sławomir; Kowal, Jerzy; Wierzbowska, Izabela; Basiaga, Marta; Nosal, Paweł; Niedbała, Piotr

    2015-01-01

    The aim of the study, conducted in the years 2011-2013, was to determine the level of gastrointestinal parasites infection in New Zealand White rabbits, kept at the Experimental Station of the University of Agriculture in Krakow. The study showed rabbits protozoan infection with the genus Eimeria, belonging - based on the sporulation method - to the following species: E. magna, E. media, E. perforans, E. stiedae and E. irresidua. The highest prevalence of infection, as well as the intensity of oocysts output (OPG - oocysts per gram of faeces), was noted for E. magna and E. media - respectively 31.4 % (19477.3 OPG), and 40.0 % (14256.07 OPG). The infection of rabbits with Eimeria spp. differed significantly between years. With regard to oocysts output, the level of infection was strongly connected with the age of rabbits, being higher in young animals. However, the range of infection was highest among adults. Among nematodes, Passalurus ambiguus pinworm was regularly found (prevalence reached 21.9%), other species - Trichuris leporis, and Graphidium strigosum were rarely noted. The overall infection with nematodes did not differ between years. Similarly, as in the case of Eimeria older individuals were more often infected by nematodes. We observed some trends in parasite oocysts/eggs output; the protozoan oocysts were recorded more often in faecal samples collected in the evenings, whereas the nematodes eggs occurred frequently in the mornings. This situation may be related to the phenomenon of coprophagy occurring in the mammals of Lagomorpha order. The results of the study indicate that especially coccidiosis constitute permanently throughout the years an important problem in the rabbitry examined.

  3. Lap belt injuries in children.

    LENUS (Irish Health Repository)

    McGrath, N

    2010-07-01

    The use of adult seat belts without booster seats in young children may lead to severe abdominal, lumbar or cervical spine and head and neck injuries. We describe four characteristic cases of lap belt injuries presenting to a tertiary children\\'s hospital over the past year in addition to a review of the current literature. These four cases of spinal cord injury, resulting in significant long-term morbidity in the two survivors and death in one child, arose as a result of lap belt injury. These complex injuries are caused by rapid deceleration characteristic of high impact crashes, resulting in sudden flexion of the upper body around the fixed lap belt, and consequent compression of the abdominal viscera between the lap belt and spine. This report highlights the dangers of using lap belts only without shoulder straps. Age-appropriate child restraint in cars will prevent these injuries.

  4. RNA transcripts of full-length cDNA clones of rabbit hepatitis E virus are infectious in rabbits.

    Science.gov (United States)

    Cossaboom, Caitlin M; Huang, Yao-Wei; Yugo, Danielle M; Kenney, Scott P; Piñeyro, Pablo; Matzinger, Shannon R; Heffron, C Lynn; Pierson, F William; Meng, Xiang-Jin

    2014-11-07

    Hepatitis E virus (HEV), the causative agent of hepatitis E, is a single-stranded positive-sense RNA virus belonging to the family Hepeviridae. At least four genotypes of the family infect humans: genotypes 1 and 2 are transmitted to humans through contaminated water, while genotypes 3 and 4 are zoonotic and have animal reservoirs. A novel strain of HEV recently identified in rabbits is a distant member of genotype 3, and thus poses a potential risk of zoonotic transmission to humans. The objective of this study was to construct and characterize an infectious cDNA clone of the rabbit HEV. Two full-length cDNA clones of rabbit HEV, pT7g-rabHEV and pT7-rabHEV, were constructed and their infectivity was tested by in vitro transfection of Huh7 human liver cells and by direct intrahepatic inoculation of rabbits with capped RNA transcripts. Results showed that positive signal for rabbit HEV protein was detected by an immunofluorescence assay with a HEV-specific antibody in Huh7 human liver cells transfected with capped RNA transcripts from the two full-length cDNA clones. Rabbits intrahepatically inoculated with capped RNA transcripts from each of the two clones developed active HEV infection as evidenced by seroconversion to anti-HEV antibodies, and detection of rabbit HEV RNA in sera and feces of inoculated animals. The availability of a rabbit HEV infectious cDNA clone now affords us the ability to delineate the mechanism of HEV replication and cross-species infection in a small animal model.

  5. SLH Timing Belt Powertrain

    Energy Technology Data Exchange (ETDEWEB)

    Schneider, Abe

    2014-04-09

    The main goal of this proposal was to develop and test a novel powertrain solution for the SLH hydroEngine, a low-cost, efficient low-head hydropower technology. Nearly two-thirds of U.S. renewable electricity is produced by hydropower (EIA 2010). According to the U.S. Department of Energy; this amount could be increased by 50% with small hydropower plants, often using already-existing dams (Hall 2004). There are more than 80,000 existing dams, and of these, less than 4% generate power (Blankinship 2009). In addition, there are over 800 irrigation districts in the U.S., many with multiple, non-power, low-head drops. These existing, non-power dams and irrigation drops could be retrofitted to produce distributed, baseload, renewable energy with appropriate technology. The problem is that most existing dams are low-head, or less than 30 feet in height (Ragon 2009). Only about 2% of the available low-head hydropower resource in the U.S. has been developed, leaving more than 70 GW of annual mean potential low-head capacity untapped (Hall 2004). Natel Energy, Inc. is developing a low-head hydropower turbine that operates efficiently at heads less than 6 meters and is cost-effective for deployment across multiple low-head structures. Because of the unique racetrack-like path taken by the prime-movers in the SLH, a flexible powertrain is required. Historically, the only viable technological solution was roller chain. Despite the having the ability to easily attach blades, roller chain is characterized by significant drawbacks, including high cost, wear, and vibration from chordal action. Advanced carbon- fiber-reinforced timing belts have been recently developed which, coupled with a novel belt attachment system developed by Natel Energy, result in a large reduction in moving parts, reduced mass and cost, and elimination of chordal action for increased fatigue life. The work done in this project affirmatively addressed each of the following 3 major uncertainties concerning

  6. Anti-coccidial effect of Commiphora molmol in the domestic rabbit (Oryctolagus cuniculus domesticus L.).

    Science.gov (United States)

    Baghdadi, Hanadi B; Al-Mathal, Ebtesam M

    2010-12-01

    This study investigated the effect of oleo-gum-resin of Commiphora monlmol Engler (Family: Burceraceae) known as Myrrh and the commercial extract known as Mirazid as a treatment against hepatic coccidiosis induced by the parasite Eimeria stiedae in domestic rabbits. Rabbits were infected with 1000 parasite sporulated oocysts and subjected to two treatment regimens, using crude myrrh suspension and the oleo-resin extract, mirazid, each administered at 500 mg/kg rabbit body weight. Treatments of infected rabbits resulted in significant reduction of the mean oocyst numbers in rabbit faeces by 52.38% in the crude-treated rabbits and by 90.90% mirazid-treated rabbits, compared to the untreated infected rabbits at day 21 post-infection (pi). At day 28 pi no oocysts parasite were observed in the faeces of rabbits. Both treatments resulted in significant recovery of infected rabbits from all symptoms of infection compared to the untreated infected group and healthy control groups. Histopathological examination of liver showed remarkable improvement in all histopathological parameters in G5 and G8 compared with the infected untreated G2. These included an almost complete healing of the hemorrhagic tissue and partial healing of the endothelial lining and hepatocytes encircling the central vein, the hepatocytes laminate regained their original radial shape and disappearance of fat vacuoles from the tissue and remarkable reduction in lymphocytes infiltration, decreased hyperplasia of the epithelial cells with significant decreasing of the parasite stage numbers. Results also indicate that mirazid was more effective than crude myrrh, probably due to higher content of purified active ingredients.

  7. Dunlop Enerka Belting supplies and installs Europe's longest conveyor belt at British Coal-Mine

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    @@ Dunlop Enerka Belting of Farington, U.K., has supplied a steel cord conveyor belt to the mine complex at Selby in North Yorkshire operated by RJB Mining plc. The new conveyor belt replaces the belt supplied and installed in 1981 by Dunlop Enerka Belting (then BTR Belting Ltd.).

  8. Coronal radiation belts

    CERN Document Server

    Hudson, H S; Frewen, S F N; DeRosa, M L

    2009-01-01

    The magnetic field of the solar corona has a large-scale dipole character, which maps into the bipolar field in the solar wind. Using standard representations of the coronal field, we show that high-energy ions can be trapped stably in these large-scale closed fields. The drift shells that describe the conservation of the third adiabatic invariant may have complicated geometries. Particles trapped in these zones would resemble the Van Allen Belts and could have detectable consequences. We discuss potential sources of trapped particles.

  9. Control of oxyuriasis in rabbits by fenbendazole.

    Science.gov (United States)

    Düwel, D; Brech, K

    1981-04-01

    Death, poor condition and unsatisfactory breeding performance in a rabbit colony was attributed to infection with Passalurus ambiguus. The trouble disappeared after treatment with 50 ppm fenbendazole in the food for 5 days. In laboratory trials 12.5 ppm for 5 days eliminated more than 99% of adult and most immature pinworms, 25 and 50 ppm eliminated all immature and adult pinworms. These doses were also effective against Obeliscoides cuniculi.

  10. Assessment of a Novel Vaccine Against Rabbit Hemorrhagic Disease Virus in Young Rabbits.

    Science.gov (United States)

    Pacho, Sonsoles; Dahdouh, Elias; Merino, Javier; Suárez, Monica

    2016-12-01

    Rabbit hemorrhagic disease virus (RHDVb) is the new variant of the classical RHDV, a virulent pathogen responsible for an acute disease in young rabbits. The virus invades internal organs, especially the liver, spleen, kidneys, and gut; prevents coagulation; and causes liver necrosis. This eventually leads to quick death of the animal because of hemorrhage. In this study, we evaluated the effects of a new vaccine against RHDVb in rabbits at a young age, after experimental infection using four different viral isolates. Our findings show that the vaccine had a protective effect with survival rates reaching 80-100% against the different isolates. These results suggest that this vaccine, when applied to young animals, is an effective tool to protect against the disease caused by RHDVb in rabbitries.

  11. Exogenous Streptococcus pneumoniae Endophthalmitis in Diabetic Rabbits

    Science.gov (United States)

    Benton, Angela H.; Fulton, Linda K.; Marquart, Mary E.

    2017-01-01

    Diabetics are at increased risk for eye infections including bacterial endophthalmitis. It is unclear whether the severity of endophthalmitis is greater in these patients due to confounding factors such as pre-existing ocular diseases in some but not others. Therefore, we tested the hypothesis that disease severity and/or bacterial loads would be significantly higher in a Type I diabetic rabbit model of Streptococcus pneumoniae endophthalmitis. Rabbits were treated with alloxan to destroy pancreatic islet cells, or mock-treated with vehicle, and maintained for 10 days before intravitreal infection with S. pneumoniae E353. Clinical scoring of the eyes was performed 24 and 48 hours after infection, followed by euthanasia and vitreous harvest to quantitate bacterial loads. There were no significant differences in clinical scores (P ≥ 0.440) or bacterial loads (P = 0.736), however, 4/12 (33%) of the diabetic rabbits became bacteremic. This finding not only indicates a breakdown in the blood-ocular barrier, but also prompts further investigation into the exploitation of the diabetic eye by the streptococci. PMID:28387365

  12. Geography of the asteroid belt

    Science.gov (United States)

    Zellner, B. H.

    1978-01-01

    The CSM classification serves as the starting point on the geography of the asteroid belt. Raw data on asteroid types are corrected for observational biases (against dark objects, for instance) to derive the distribution of types throughout the belt. Recent work on family members indicates that dynamical families have a true physical relationship, presumably indicating common origin in the breakup of a parent asteroid.

  13. Effects of myxoma virus and rabbit hemorrhagic disease virus on the physiological condition of wild European rabbits: Is blood biochemistry a useful monitoring tool?

    Science.gov (United States)

    Pacios-Palma, Isabel; Santoro, Simone; Bertó-Moran, Alejandro; Moreno, Sacramento; Rouco, Carlos

    2016-12-01

    Myxomatosis and rabbit hemorrhagic disease (RHD) are the major viral diseases that affect the wild European rabbit (Oryctolagus cuniculus). These diseases arrived in Europe within the last decades and have caused wild rabbit populations to decline dramatically. Both viruses are currently considered to be endemic in the Iberian Peninsula; periodic outbreaks that strongly impact wild populations regularly occur. Myxoma virus (MV) and rabbit hemorrhagic disease virus (RHDV) alter the physiology of infected rabbits, resulting in physical deterioration. Consequently, the persistence and viability of natural populations are affected. The main goal of our study was to determine if blood biochemistry is correlated with serostatus in wild European rabbits. We carried out seven live-trapping sessions in three wild rabbit populations over a two-year period. Blood samples were collected to measure anti-MV and anti-RHDV antibody concentrations and to measure biochemical parameters related to organ function, protein metabolism, and nutritional status. Overall, we found no significant relationships between rabbit serostatus and biochemistry. Our main result was that rabbits that were seropositive for both MV and RHDV had low gamma glutamyltransferase concentrations. Given the robustness of our analyses, the lack of significant relationships may indicate that the biochemical parameters measured are poor proxies for serostatus. Another explanation is that wild rabbits might be producing attenuated physiological responses to these viruses because the latter are now enzootic in the study area.

  14. Relationship Between the Inoculum Dose of Streptococcus pneumoniae and Pneumonia Onset in a Rabbit Model

    Science.gov (United States)

    2005-04-01

    White rabbits (mean + or - SD + or - 4.75 + or - 0.25 kg (n = 27)). Rabbits were endobronchially inoculated with increasing doses of Streptococcus ... pneumoniae and pneumonia onset was observed over the following 96 h. The diagnostic approach was based on the Clinical Pulmonary Infection Score

  15. Evaluation of the static belt fit provided by belt-positioning booster seats.

    Science.gov (United States)

    Reed, Matthew P; Ebert, Sheila M; Sherwood, Christopher P; Klinich, Kathleen D; Manary, Miriam A

    2009-05-01

    Belt-positioning booster seats are recommended for children who use vehicle seat belts as primary restraints but who are too small to obtain good belt fit. Previous research has shown that belt-positioning boosters reduce injury risk, but the belt fit produced by the wide range of boosters in the US market has not previously been assessed. The present study describes the development of a method for quantifying static belt fit with a Hybrid-III 6-year-old test dummy. The measurement method was applied in a laboratory seat mockup to 31 boosters (10 in both backless and highback modes) across a range of belt geometries obtained from in-vehicle measurements. Belt fit varied widely across boosters. Backless boosters generally produced better lap belt fit than highback boosters, largely because adding the back component moved the dummy forward with respect to the lap belt routing guides. However, highback boosters produced more consistent shoulder belt fit because of the presence of belt routing guides near the shoulder. Some boosters performed well on both lap belt and shoulder belt fit. Lap belt fit in dedicated boosters was generally better than in combination restraints that also can be used with an integrated harness. Results demonstrate that certain booster design features produce better belt fit across a wide range of belt geometries. Lap belt guides that hold the belt down, rather than up, and shoulder belt guides integrated into the booster backrest provided better belt fit.

  16. Hepatitis E Virus in Farmed Rabbits, Wild Rabbits and Petting Farm Rabbits in the Netherlands

    NARCIS (Netherlands)

    Burt, Sara A.; Veltman, Jorg; Hakze-van der Honing, Renate; Schmitt, Heike; Poel, van der Wim H.M.

    2016-01-01

    Rabbits have been suggested as a zoonotic source of Hepatitis E virus. Phylogenetic analysis of HEV isolates from farmed, wild and pet rabbits in the Netherlands (23, 0, and 60 % respectively) showed them to be grouped amongst published rabbit HEV sequences and distinct from most human isolates.

  17. Hepatitis E Virus in Farmed Rabbits, Wild Rabbits and Petting Farm Rabbits in the Netherlands

    NARCIS (Netherlands)

    Burt, S.A.; Veltman, Jorg; Hakze-van der Honing, Renate; Schmitt, Heike; van der Poel, Wim H.M.

    2016-01-01

    Rabbits have been suggested as a zoonotic source of Hepatitis E virus. Phylogenetic analysis of HEV isolates from farmed, wild and pet rabbits in the Netherlands (23, 0, and 60 % respectively) showed them to be grouped amongst published rabbit HEV sequences and distinct from most human isolates. Dut

  18. CHRONIC EXPOSURE TO DIBROMOACETIC ACID, A WATER DISINFECTION BY-PRODUCT, DIMINISHES PRIMORDIAL FOLLICLE POPULATIONS IN THE RABBIT

    Science.gov (United States)

    To determine if dibromoacetic acid (DBA) affects folliculogenesis, four groups of female Dutch-belted rabbits were exposed daily to 0, 1, 5, or 50 mg DBA/kg body wt. in drinking water beginning in utero from gestation day 15 throughout life. Functionality of the endocrine axis w...

  19. 蒿甲醚或吡喹酮早期治疗感染血吸虫尾蚴兔和犬的肝脏显微镜检查%Microscopic observations on livers of rabbits and dogs infected with Schistosoma japonicum cercariae and early treatment with artemether or praziquantel

    Institute of Scientific and Technical Information of China (English)

    肖树华; 杨元清; 张超威; 尤纪青

    1996-01-01

    To study the histopathological change of the liver of the hosts treated with artemether (Art) or praziquantel (Pra) in early stage after infection with Schistosoma japonicum cercariae. METHODS:Dogs infected once with schistosome cercariae were by the repeated dosing once every 1 or 2 wk for 2 -4 times. In rabbits, infected with 48 - 52 schistosome cercariae once every other day for 5 times,infection, followed by the repeated dosing every 1 or 2 wk for 2 - 3 times. RESULTS: After above mentioned dogs or rabbits were treated ig with Art,ArtC or Pra, the female worm reduction rates were 92.1% - 100 %. Histopathological examination showed that the reduction rates of total granuloma in the liver sections of the dogs and rabbits were 70.9 % - 97.3 % and 76.5 % - 97.4 %, respectively. Meantime, the structure of the hepatic lobules was normal with normal arrangement of the liver bundle. CoNCLusIoNS: Early treatment with Art or Pra exhibited a promising effect of protection of the liver of the dogs and rabbits infected with schistosome cercariae.%观察感染血吸虫尾蚴后早期用蒿甲醚(Art)或吡喹酮(Pra)治疗,对宿主肝组织的影响.方法:犬感染198-202条尾蚴后d 7ig Art 10 mg·kg-1,Art胶囊(ArtC)15 mg·kg-1或感染后d 21ig Pra 30-40 mg·kg-1,1-2 wk重复给药1次,共2-4次;兔每隔日感染48-52条尾蚴,共5次,并于第1次感染后d 7或d 21 ig 上述剂量的Art和Pra,停药后4-5 wk剖杀取肝作切片观察.结果:犬与兔经Art或Pra早期治疗后,减♀虫率达92.1%-100%,肝切片中的总虫卵肉芽肿数分别减少70.9%-97.3%和76.5%-97.4%,肝叶结构和肝索排列正常.结论:2种药物早期治疗对宿主肝脏有保护作用.

  20. Infections

    Science.gov (United States)

    ... Does My Child Need? How to Safely Give Acetaminophen Is It a Cold or the Flu? Is the Flu Vaccine a Good Idea for Your Family? Too Late for the Flu Vaccine? Common Childhood Infections Can Chronic Ear Infections Cause Long-Term Hearing Loss? Chickenpox Cold Sores Common Cold Diarrhea Fever and ...

  1. Seat Belt Use and Stress in Adolescents.

    Science.gov (United States)

    Schichor, Aric; And Others

    1990-01-01

    Explored adolescent seat belt use and psychosocial risk factors in urban minority population (n=541). Found seat belt use reported by 49 percent of respondents. Those reporting no or intermittent seat belt use were significantly more likely than seat belt users to feel down, have decreased home support, have problems with school and the law, and…

  2. Rabbit Repellent Paint

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Five gallons of rabbit repellent paint were sent to George Wilson to be applied on the trees of the Tewaukon tree plot. Mr. Wilson requires a 3 or 4 in. brush for...

  3. The Cutaneous Rabbit Revisited

    Science.gov (United States)

    Flach, Rudiger; Haggard, Patrick

    2006-01-01

    In the cutaneous rabbit effect (CRE), a tactile event (so-called attractee tap) is mislocalized toward an adjacent attractor tap. The effect depends on the time interval between the taps. The authors delivered sequences of taps to the forearm and asked participants to report the location of one of the taps. The authors replicated the original CRE…

  4. A Clever Rabbit

    Institute of Scientific and Technical Information of China (English)

    呼振璞; 付晓明

    2002-01-01

    1. Once there lived an elephant and a lion in the mountains, who both wanted to be the king of the beasts. One day the lion caught a rabbit. Before he ate it, he said, “Do you know I am the king here? ”

  5. 49 CFR 393.93 - Seats, seat belt assemblies, and seat belt assembly anchorages.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Seats, seat belt assemblies, and seat belt... § 393.93 Seats, seat belt assemblies, and seat belt assembly anchorages. (a) Buses—(1) Buses... the driver's seat and seat belt assembly anchorages that conform to the location and...

  6. Effects of experimental infections with larvae of Eustrongylides ignotus Jäegerskiold, 1909 and Contracaecum multipapillatum (Drasche, 1882 Baylis, 1920 in rabbits Efeitos de infecções experimentais em coelhos com larvas de Eustrongylides ignotus Jäegerskiold, 1909 e Contracaecum multipapillatum (Drasche, 1882 Baylis, 1920

    Directory of Open Access Journals (Sweden)

    L.A. Barros

    2004-06-01

    Full Text Available Rabbits were infected per os with 10 Eustrongylides ignotus L4 and with 50 Contracaecum multipapillatum L3 per rabbit, recovered from naturally infected freshwater fishes (Hoplias malabaricus in order to evaluate the patogenicity of these two nematode species in mammalian host. Two rabbits (20% infected with E. ignotus died before the fourth day post-inoculation (one after 51 and the other after 78 hours. Six rabbits (60% were inappetent until the fifth day following experimental inoculation. No clinical signs in rabbits inoculated with C. multipapillatum were observed; nevertheless, eight (80% animals were positive for this nematode species. Rabbits inoculated with E. ignotus, had gastric congestion with hematoma of the gastric wall in 60% of the cases. Peritoneum was congested in 20% of the animals with the presence of peritoneal abscess in 10% of the cases. All inoculated animals showed hyperemia of the gastric mucosa with hemorrhagic gastritis due to infections with E. ignotus. In C. multipapillatum inoculated animals, the hyperemia was followed by disruption of the epithelial mucosa in the sites of parasite attachment. In the gastric mucosa, miscellaneous leukocitary infiltrates, with multifocal necrosis reaching the submucosa in the infections with C. multipapillatum were observed under bright field microscopy. Perforating lesions in several organs, mainly in the gastric wall, pancreas and liver, always in the presence of a mixed inflammatory process, intensely fibrous, with hemorrhage and necrosis were observed in animals infected with E. ignotus.Coelhos foram infectados experimentalmente per os com 10 larvas L4 de Eustrogylides ignotus (n= 10 e 50 L3 de Contracaecum multipapillatum (n= 50 coletados em traíras (Hoplias malabaricus naturalmente parasitadas a fim de se avaliar a patogenicidade induzida por essas espécies de nematóides em mamíferos. Dois coelhos (20% infectados com E. ignotus morreram antes do quarto dia p

  7. Chaos on the conveyor belt

    CERN Document Server

    Sándor, Bulcsú; Tél, Tamás; Néda, Zoltán

    2013-01-01

    The dynamics of a spring-block train placed on a moving conveyor belt is investigated both by simple experiments and computer simulations. The first block is connected by spring to an external static point, and due to the dragging effect of the belt the blocks undergo complex stick-slip dynamics. A qualitative agreement with the experimental results can only be achieved by taking into account the spatial inhomogeneity of the friction force on the belt's surface, modeled as noise. As a function of the velocity of the conveyor belt and the noise strength, the system exhibits complex, self-organized critical, sometimes chaotic dynamics and phase transition-like behavior. Noise induced chaos and intermittency is also observed. Simulations suggest that the maximum complexity of the dynamical states is achieved for a relatively small number of blocks, around five.

  8. Activity of diclazuril against coccidiosis in growing rabbits: experimental and field experiences

    Directory of Open Access Journals (Sweden)

    Monita Vereecken

    2012-12-01

    Full Text Available The efficacy of diclazuril in growing rabbits was investigated under experimental and field conditions. In a first experimental trial, the susceptibility of recent isolated French Eimeria field strains to in-feed use of diclazuril, salinomycin and robenidine was studied in fattening rabbits. Rabbits were challenged at the age of 31 d with a mixed inoculum of Eimeria magna, E. media and E. perforans. Production data and oocyst excretion were compared with an infected-untreated control group and an uninfected-untreated control group. Infection resulted in significantly lower production data and higher oocyst excretion in the infected-untreated control group. Salinomycin and diclazuril treated rabbits were able to control the infection, demonstrated also by comparable weight gain and final weight to those of the uninfected-untreated control rabbits and significantly higher than those of the infected-untreated control rabbits. Based on the production data and oocyst excretion, robenidine was not able to control the infection adequately. Economic performance (weight gain, feed intake, feed conversion and oocyst excretion were significantly worse than in the uninfected-untreated controls. In a second trial, a 1 yr longitudinal study was carried out in Italy to evaluate the excretion of coccidia in growing rabbits from 8 meat farms applying a 2-phase anticoccidial programme (diclazuril and robenidine. Parasitological parameters (oocyst counts and species identification were measured monthly. Seven of the 11 known coccidial rabbit species were identified. Variable levels of oocysts per gram were detected in the farms, but on all farms lower oocyst per gram and a reduced number of Eimeria spp. in rabbit faeces were recorded in the 8-mo treatment period with diclazuril.

  9. Northern Belt of Jupiter

    Science.gov (United States)

    2000-01-01

    [figure removed for brevity, see original site] A four-panel frame shows a section of Jupiter's north equatorial belt viewed by NASA's Cassini spacecraft at four different wavelengths, and a separate reference frame shows the location of the belt on the planet.A fascinating aspect of the images in the four-panel frame is the small bright spot in the center of each. The images come from different layers of the atmosphere, so the spot appears to be a storm penetrating upward through several layers. This may in fact be a 'monster' thunderstorm, penetrating all the way into the stratosphere, as do some summer thunderstorms in the midwestern United States. These images were taken on Nov. 27, 2000, at a resolution of 192 kilometers (119 miles) per pixel. They have been contrast-enhanced to highlight features in the atmosphere.The top panel of the four-panel frame is an image taken in a near-infrared wavelength at which the gases in Jupiter's atmosphere are relatively non-absorbing. Sunlight can penetrate deeply into the atmosphere at this wavelength and be reflected back out, providing a view of an underlying region of the atmosphere, the lower troposphere.The second panel was taken in the blue portion of wavelengths detected by the human eye. At these wavelengths, gases in the atmosphere scatter a modest amount of sunlight, so the clouds we see tend to be at somewhat higher altitudes than in the top panel.The third panel shows near-infrared reflected sunlight at a wavelength where the gas methane, an important constituent of Jupiter's atmosphere, absorbs strongly. Dark places are regions without high-level clouds and consequently large amounts of methane accessible to sunlight. Bright regions are locations with high clouds in the upper troposphere shielding the methane below.The bottom panel was taken in the ultraviolet. At these very short wavelengths, the clear atmosphere scatters sunlight, and hazes in the stratosphere, above the troposphere, absorb sunlight. That

  10. [Targeted modification of CCR5 gene in rabbits by TALEN].

    Science.gov (United States)

    Tang, Chengcheng; Zhang, Quanjun; Li, Xiaoping; Fan, Nana; Yang, Yi; Quan, Longquan; Lai, Liangxue

    2014-04-01

    The lack of suitable animal model for HIV-1 infection has become a bottleneck for the development of AIDS vaccines and drugs. Wild-type rabbits can be infected by HIV-1 persistently and HIV-1 can be efficiently replicated resulting in syncytia in rabbit cell line co-expressing human CD4 and CCR5.Therefore, a rabbit highly expressing human CD4 and CCR5 may be an ideal animal model for AIDS disease study. In the present report, by using the efficient gene targeting technology, transcription activator-like effector nuclease (TALEN), we explored the feasibility of generating a HIV-1 model by knocking in human CD4 and CCR5 into rabbit genome. First we constructed two TALEN vectors targeting rabbit CCR5 gene and a vector with homologous arms. TALEN mRNAs and donor DNA were then co-injected into fertilized oocytes. After 3?5 days, 24 embryos were collected and used to conduct mutation analysis with PCR and sequencing. All the 24 embryos were detected with CCR5 knockouts and 5 were human CD4 and CCR5 knockins. Our results laid a foundation for establishing a new animal model for the study of AIDS.

  11. Infections

    Directory of Open Access Journals (Sweden)

    Virginia Vanzzini Zago

    2012-01-01

    Full Text Available This is a retrospective, and descriptive study about the support that the laboratory of microbiology aids can provide in the diagnosis of ocular infections in patients whom were attended a tertiary-care hospital in México City in a 10-year-time period. We describe the microbiological diagnosis in palpebral mycose; in keratitis caused by Fusarium, Aspergillus, Candida, and melanized fungi; endophthalmitis; one Histoplasma scleritis and one mucormycosis. Nowadays, ocular fungal infections are more often diagnosed, because there is more clinical suspicion and there are easy laboratory confirmations. Correct diagnosis is important because an early medical treatment gives a better prognosis for visual acuity. In some cases, fungal infections are misdiagnosed and the antifungal treatment is delayed.

  12. The researchers developed luminous rabbit

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    <正>Their efforts produced two rabbits out of a litter of eight that went from being a normal, fluffy(蓬松的) white to glowing green in the dark. The rabbits were born at the University of Istanbul as part of a collaboration between scientists from universities in Turkey and Hawaii. The rabbits glow to show that a genetic manipulation technique can work efficiently,

  13. Effects of vehicle seat and belt geometry on belt fit for children with and without belt positioning booster seats.

    Science.gov (United States)

    Reed, Matthew P; Ebert-Hamilton, Sheila M; Klinich, Kathleen D; Manary, Miriam A; Rupp, Jonathan D

    2013-01-01

    A laboratory study was conducted to quantify the effects of belt-positioning boosters on lap and shoulder belt fit. Postures and belt fit were measured for forty-four boys and girls ages 5-12 in four highback boosters, one backless booster, and on a vehicle seat without a booster. Belt anchorage locations were varied over a wide range. Seat cushion angle, seat back angle, and seat cushion length were varied in the no-booster conditions. All boosters produced better mean lap belt fit than was observed in the no-booster condition, but the differences among boosters were relatively large. With one midrange belt configuration, the lap belt was not fully below the anterior-superior iliac spine (ASIS) landmark on the front of the pelvis for 89% of children in one booster, and 75% of children failed to achieve that level of belt fit in another. In contrast, the lap belt was fully below the ASIS for all but two children in the best-performing booster. Child body size had a statistically significant but relatively small effect on lap belt fit. The largest children sitting without a booster had approximately the same lap belt fit as the smallest children experienced in the worst-performing booster. Increasing lap belt angle relative to horizontal produced significantly better lap belt fit in the no-booster condition, but the boosters isolated the children from the effects of lap belt angles. Reducing seat cushion length in the no-booster condition improved lap belt fit but changing cushion angle did not. Belt upper anchorage (D-ring) location had a strong effect on shoulder belt fit in conditions without shoulder belt routing from the booster. Unexpectedly, the worst average shoulder belt fit was observed in one highback booster with a poorly positioned shoulder belt routing clip. The shoulder belt was routed more outboard, on average, with a backless booster than without a booster, but raising the child also amplified the effect of D-ring location, such that children were

  14. Ultrastructure of Reissner's membrane in the rabbit

    DEFF Research Database (Denmark)

    Qvortrup, K.; Rostgaard, Jørgen; Bretlau, P.

    1994-01-01

    Anatomy, Reissner's membrane, electron microscopy, tubulocisternal endoplasmic reticulum, subsurface cisterns, rabbit......Anatomy, Reissner's membrane, electron microscopy, tubulocisternal endoplasmic reticulum, subsurface cisterns, rabbit...

  15. Kuiper Belts Around Nearby Stars

    CERN Document Server

    Nilsson, R; Brandeker, A; Olofsson, G; Pilbratt, G L; Risacher, C; Rodmann, J; Augereau, J -C; Bergman, P; Eiroa, C; Fridlund, M; Thébault, P; White, G J

    2010-01-01

    In order to detect and characterise cold extended circumstellar dust originating from collisions of planetesimal bodies in disks, belts, or rings at Kuiper-Belt distances (30--50\\,AU or beyond) sensitive submillimetre observations are essential. Measurements of the flux densities at these wavelengths will extend existing IR photometry and permit more detailed modelling of the Rayleigh-Jeans tail of the disks spectral energy distribution (SED), effectively constraining dust properties and disk extensions. By observing stars spanning from a few up to several hundred Myr, the evolution of debris disks during crucial phases of planet formation can be studied. // We have performed 870\\,$\\mu$m observations of 22 exo-Kuiper-Belt candidates, as part of a Large Programme with the LABOCA bolometer at the APEX telescope. Dust masses (or upper limits) were calculated from integrated 870\\,$\\mu$m fluxes, and fits to the SED of detected sources revealed the fractional dust luminosities $f_{\\mathrm{dust}}$, dust temperatures...

  16. Infection

    Science.gov (United States)

    2010-09-01

    Interactions between biofilms and the environment. FEMS Microbiol Rev. 1997;20:291–303. 4. Webb LX, Wagner W, Carroll D, et al. Osteomyelitis and...treatment of osteomyelitis . Biomed Mater. 2008;3: 034114. 6. Gristina AG. Biomaterial-centered infection: microbial adhesion versus tissue integration...vertebral osteomyelitis . Spine. 2007;32: 2996–3006. 15. Beckham JD, Tuttle K, Tyler KL. Reovirus activates transforming growth factor ß and bone

  17. Pea-derived vaccines demonstrate high immunogenicity and protection in rabbits against rabbit haemorrhagic disease virus.

    Science.gov (United States)

    Mikschofsky, Heike; Schirrmeier, Horst; Keil, Günther M; Lange, Bodo; Polowick, Patricia L; Keller, Wilf; Broer, Inge

    2009-08-01

    Vaccines against rabbit haemorrhagic disease virus (RHDV) are commercially produced in experimentally infected rabbits. A genetically engineered and manufactured version of the major structural protein of RHDV (VP60) is considered to be an alternative approach for vaccine production. Plants have the potential to become an excellent recombinant production system, but the low expression level and insufficient immunogenic potency of plant-derived VP60 still hamper its practical use. In this study, we analysed the expression of a novel multimeric VP60-based antigen in four different plant species, including Nicotiana tabacum L., Solanum tuberosum L., Brassica napus L. and Pisum sativum L. Significant differences were detected in the expression patterns of the novel fusion antigen cholera toxin B subunit (CTB)::VP60 (ctbvp60(SEKDEL)) at the mRNA and protein levels. Pentameric CTB::VP60 molecules were only detected in N. tabacum and P. sativum, and displayed equal levels of CTB, at approximately 0.01% of total soluble protein (TSP), and traces of detectable VP60. However, strong enhancement of the CTB protein content via self-fertilization was only observed in P. sativum, where it reached up to 0.7% of TSP. In rabbits, a strong decrease in the protective vaccine dose required from 48-400 microg potato-derived VP60 [Castanon, S., Marin, M.S., Martin-Alonso, J.M., Boga, J.A., Casais, R., Humara, J.M., Ordas, R.J. and Parra, F. (1999) Immunization with potato plants expressing VP60 protein protects against rabbit hemorrhagic disease virus. J. Virol. 73, 4452-4455; Castanon, S., Martin-Alonso, J.M., Marin, M.S., Boga, J.A., Alonso, P., Parra, F. and Ordas, R.J. (2002) The effect of the promoter on expression of VP60 gene from rabbit hemorrhagic disease virus in potato plants. Plant Sci. 162, 87-95] to 0.56-0.28 microg antigenic VP60 (measured with VP60 enzyme-linked immunosorbent assay) of crude CTB::VP60 pea extracts was demonstrated. Rabbits immunized with pea-derived CTB

  18. The Year of the Rabbit

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Every year of the Chinese lunar calendar corresponds with an animal. The rat,ox, tiger, rabbit, dragon, snake, horse,sheep, monkey, rooster, dog and pig make up the Chinese zodiac, which repeats in a12-year cycle. This year’s Chinese NewYear rings in the Year of the Rabbit.

  19. The White Rabbit project

    CERN Document Server

    Serrano, J; Gousiou, E; van der Bij, E; Wlostowski, T; Daniluk, G; Lipinski, M

    2013-01-01

    White Rabbit (WR) is a multi-laboratory, multi- company collaboration for the development of a new Ethernet-based technology which ensures sub-nanosecond synchronisation and deterministic data transfer. The project uses an open source paradigm for the development of its hardware, gateware and software components. This article provides an introduction to the technical choices and an explanation of the basic principles underlying WR. It then describes some possible applications and the current status of the project. Finally, it provides insight on current developments and future plans.

  20. French experience in seat belt use.

    NARCIS (Netherlands)

    Lassarre, S. & Page, Y.

    1992-01-01

    This paper concerns the French experience in seat belt use. As well as the seat belt regulations, the strategies employed to reinforce the wearing of seat belts by using information and encouragement campaigns and checks by the police and gendarmerie are described here along with their timetables an

  1. Appendiceal transection associated with seat belt restraint.

    Science.gov (United States)

    Go, Seung Je; Sul, Young Hoon; Ye, Jin Bong; Kim, Joong Suck

    2016-08-01

    The seat belt is designed for safety in a motor vehicle and should be worn to prevent severe injuries. But, the seat belt itself can be an injury factor in combination with deceleration forces applied to fixation points of mobile viscera. Here, we present a 23-year-man with traumatic transection of the appendix, highly mobile viscera, following seat belt injury.

  2. The rabbit as an experimental model in laryngology

    Directory of Open Access Journals (Sweden)

    Carneiro, Christiano de Giacomo

    2009-06-01

    Full Text Available Introduction: In the research in laryngology we normally use animal models. The animal experimentation may also contribute largely for this evolution, mainly for the easy access compared to human larynxes and for they are more easily controlled. Objective: The objective of this work is to analyze the laryngofissure with vocal cords graft as an experimental surgical technique in male adult rabbits. Method: We studied 46 New Zealand albino rabbits submitted to microsurgery in both vocal cords with autologous unilateral or bilateral graft of fat or fascia. Results: There were 4 losses of 3 animals until the first week of the postoperative period and another after 19 days after surgery. In the subsequent animals there were no infection, hematoma or sutures dehiscence. Conclusion: The study enables the conclusion that the experimental laryngofissure in rabbits is a safe method that may be used for laryngological studies.

  3. Challenges in the rabbit haemorrhagic disease 2 (RHDV2) molecular diagnosis of vaccinated rabbits.

    Science.gov (United States)

    Carvalho, C L; Duarte, E L; Monteiro, M; Botelho, A; Albuquerque, T; Fevereiro, M; Henriques, A M; Barros, S S; Duarte, Margarida Dias

    2017-01-01

    Molecular methods are fundamental tools for the diagnosis of viral infections. While interpretation of results is straightforward for unvaccinated animals, where positivity represents ongoing or past infections, the presence of vaccine virus in the tissues of recently vaccinated animals may mislead diagnosis. In this study, we investigated the interference of RHDV2 vaccination in the results of a RT-qPCR for RHDV2 detection, and possible associations between mean Cq values of five animal groups differing in age, vaccination status and origin (domestic/wild). Viral sequences from vaccinated rabbits that died of RHDV2 infection (n=14) were compared with the sequences from the commercial vaccines used in those animals. Group Cq means were compared through Independent t-test and One-way ANOVA. We proved that RHDV2 vaccine-RNA is not detected by the RT-qPCR as early as 15days post-vaccination, an important fact in assisting results interpretation for diagnosis. Cq values of vaccinated and non-vaccinated infected domestic adults showed a statistically significant difference (p<0.05), demonstrating that vaccination-induced immunity reduces viral loads and delays disease progression. Contrarily, in vaccinated young rabbits higher viral loads were registered compared to non-vaccinated kittens. No significant variation (p=0.3824) was observed between viral loads of non-vaccinated domestic and wild RHDV2-victimised rabbits. Although the reduced number of vaccinated young animals analysed hampered a robust statistical analysis, this occurrence suggests that passively acquired maternal antibodies may inhibit the active immune response to vaccination, delaying protection and favouring disease progression. Our finding emphasises the importance of adapting kitten RHDV2 vaccination schedules to circumvent this interference phenomenon.

  4. Is the new variant RHDV replacing genogroup 1 in Portuguese wild rabbit populations?

    Science.gov (United States)

    Lopes, Ana M; Correia, Jorge; Abrantes, Joana; Melo, Pedro; Ramada, Margarida; Magalhães, Maria J; Alves, Paulo C; Esteves, Pedro J

    2014-12-30

    The Lagovirus rabbit hemorrhagic disease virus (RHDV), a member of the family Caliciviridae, severely affects European rabbit (Oryctolagus cuniculus) populations by causing rabbit hemorrhagic disease (RHD). RHDV is subdivided in six genogroups but, more recently, a new RHDV variant with a unique genetic and antigenic profile emerged. We performed a study in rabbits found dead in the field during 2013 and 2014 in Portugal to determine the prevalence of this new variant versus the classical RHDV. Fifty-seven liver samples were screened for the presence of RHDV and positive samples were genotyped. All cases of RHDV infection were caused by the new variant. The only former genogroup circulating in Portugal, G1, was not detected. We hence conclude that the new RHDV variant is replacing G1 in Portugal, probably due to a selective advantage. This sudden and rapid replacement emphasizes the necessity of continued monitoring of wild rabbit populations.

  5. Is the New Variant RHDV Replacing Genogroup 1 in Portuguese Wild Rabbit Populations?

    Science.gov (United States)

    Lopes, Ana M.; Correia, Jorge; Abrantes, Joana; Melo, Pedro; Ramada, Margarida; Magalhães, Maria J.; Alves, Paulo C.; Esteves, Pedro J.

    2014-01-01

    The Lagovirus rabbit hemorrhagic disease virus (RHDV), a member of the family Caliciviridae, severely affects European rabbit (Oryctolagus cuniculus) populations by causing rabbit hemorrhagic disease (RHD). RHDV is subdivided in six genogroups but, more recently, a new RHDV variant with a unique genetic and antigenic profile emerged. We performed a study in rabbits found dead in the field during 2013 and 2014 in Portugal to determine the prevalence of this new variant versus the classical RHDV. Fifty-seven liver samples were screened for the presence of RHDV and positive samples were genotyped. All cases of RHDV infection were caused by the new variant. The only former genogroup circulating in Portugal, G1, was not detected. We hence conclude that the new RHDV variant is replacing G1 in Portugal, probably due to a selective advantage. This sudden and rapid replacement emphasizes the necessity of continued monitoring of wild rabbit populations. PMID:25559218

  6. Seat belt sign and its significance.

    Science.gov (United States)

    Agrawal, Amit; Inamadar, Praveenkumar Ishwarappa; Subrahmanyam, Bhattara Vishweswar

    2013-07-01

    Safety belts are the most important safety system in motor vehicles and when worn intend to prevent serious injuries. However, in unusual circumstances (high velocity motor vehicle collisions) these safety measures (seat belts) can be the source and cause of serious injuries. The seat belt syndrome was first described as early by Garrett and Braunste in but the term "seat belt sign" was discussed by Doersch and Dozier. Medical personnel's involved in emergency care of trauma patients should be aware of seat belt sign and there should a higher index of suspicion to rule out underlying organ injuries.

  7. Rabbit and human hepatitis E virus strains belong to a single serotype.

    Science.gov (United States)

    Wang, Song; Cheng, Xianfeng; Dai, Xing; Dong, Chen; Xu, Mingjie; Liang, Jiuhong; Dong, Min; Purdy, Michael A; Meng, Jihong

    2013-09-01

    Hepatitis E virus (HEV) is a zoonotic pathogen and all four established genotypes of HEV belong to a single serotype. The recently identified rabbit HEV is antigenically and genetically related to human HEV. It is unclear whether rabbit HEV belongs to the same serotype as human HEV. The purpose of this study was to determine the serotypic relationship between rabbit and human HEVs. HEV ORF2 recombinant capsid protein p166 (amino acids 452-617) of four known HEV genotypes and rabbit HEV were used to induce immune serum, which were evaluated for their ability to neutralize human HEV genotype 1, 4, and rabbit HEV strains by an in vitro PCR-based HEV neutralization assay. Immune sera of five kinds of p166 proteins were all found to neutralize or cross-neutralize the three different HEV strains, suggesting a common neutralization epitope(s) existing between human and rabbit HEV. Rabbit models of a second-passage rabbit HEV strain, JS204-2, and a genotype 4 human HEV strain, NJ703, were established as evidenced by fecal virus shedding, viremia and anti-HEV IgG seroconversion. Six rabbits, recovered from JS204 infection, were challenged with NJ703, and another six recovered from NJ703 infection were challenged with JS204-2. After challenge, viremia was not detected, shorter fecal virus shedding durations and obvious early stage declines in anti-HEV IgG values were observed. The results from this study indicate that rabbit HEV belongs to the same serotype as human HEV.

  8. Oculoscopy in Rabbits and Rodents.

    Science.gov (United States)

    Jekl, Vladimir; Hauptman, Karel; Knotek, Zdenek

    2015-09-01

    Ophthalmic diseases are common in rabbits and rodents. Fast and definitive diagnosis is imperative for successful treatment of ocular diseases. Ophthalmic examination in rabbits and rodents can be challenging. Oculoscopy offers great magnification for the examination of the ocular structures in such animals, including the evaluation of cornea, anterior eye chamber, limbus, iris, lens, and retina. To date, oculoscopy has been described only sporadically and/or under experimental conditions. This article describes the oculoscopy technique, normal and abnormal ocular findings, and the most common eye disorders diagnosed with the aid of endoscopy in rabbits and rodents.

  9. Can an Antibiotic (Macrolide) Prevent Chlamydia pneumoniae-Induced Atherosclerosis in a Rabbit Model?

    Science.gov (United States)

    Fong, Ignatius W.; Chiu, Brian; Viira, Esther; Jang, Dan; Fong, Michael W.; Peeling, Rosanna; Mahony, James B.

    1999-01-01

    There is increasing data implicating Chlamydia pneumoniae in the pathogenesis of atherosclerosis, and antibiotics may theoretically be useful to prevent secondary vascular complications. Three groups of New Zealand White specific-pathogen-free rabbits, fed cholesterol-free chow, were inoculated via the nasopharynx on three occasions, 2 weeks apart, with C. pneumoniae. Group I (n = 23) rabbits were untreated; group II (n = 24) rabbits were treated with azithromycin at 30 mg/kg of body weight daily for 3 days and then once every 6 days, starting 5 days after first inoculation and continuing until sacrifice (early treatment); and group III (n = 24) rabbits were treated with the same dose of azithromycin but initiated 2 weeks after the last inoculation. All animals were sacrificed at 10 to 11 weeks after initial inoculation and examined for signs of atherosclerosis of the aorta. Eight (34.8%) untreated rabbits developed early signs of atherosclerosis, whereas only one (4.2%) in the early-treatment group had such signs (P = 0.02). However, eight rabbits (33.3%) of the delayed-treatment group had atherosclerotic changes of the aorta and no significant reduction compared to untreated rabbits. Early treatment of C. pneumoniae-infected rabbits with azithromycin was highly effective (87%) in preventing atherosclerotic changes, but delayed treatment was ineffective. It is possible that longer or more aggressive antibiotic treatment may be needed to reverse preformed lesions or that antibiotics may not be of value once lesions have formed. PMID:10548582

  10. Potential Vaccine Targets against Rabbit Coccidiosis by Immunoproteomic Analysis.

    Science.gov (United States)

    Song, Hongyan; Dong, Ronglian; Qiu, Baofeng; Jing, Jin; Zhu, Shunxing; Liu, Chun; Jiang, Yingmei; Wu, Liucheng; Wang, Shengcun; Miao, Jin; Shao, Yixiang

    2017-02-01

    The aim of this study was to identify antigens for a vaccine or drug target to control rabbit coccidiosis. A combination of 2-dimensional electrophoresis, immunoblotting, and mass spectrometric analysis were used to identify novel antigens from the sporozoites of Eimeria stiedae. Protein spots were recognized by the sera of New Zealand rabbits infected artificially with E. stiedae. The proteins were characterized by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS) analysis in combination with bioinformatics. Approximately 868 protein spots were detected by silver-staining, and a total of 41 immunoreactive protein spots were recognized by anti-E. stiedae sera. Finally, 23 protein spots were successfully identified. The proteins such as heat shock protein 70 and aspartyl protease may have potential as immunodiagnostic or vaccine antigens. The immunoreactive proteins were found to possess a wide range of biological functions. This study is the first to report the proteins recognized by sera of infected rabbits with E. stiedae, which might be helpful in identifying potential targets for vaccine development to control rabbit coccidiosis.

  11. Ring current and radiation belts

    Science.gov (United States)

    Williams, D. J.

    1987-01-01

    Studies performed during 1983-1986 on the ring current, the injection boundary model, and the radiation belts are discussed. The results of these studies yielded the first observations on the composition and charge state of the ring current throughout the ring-current energy range, and strong observational support for an injection-boundary model accounting for the origins of radiation-belt particles, the ring current, and substorm particles observed at R less than about 7 earth radii. In addition, the results have demonstrated that the detection of energetic neutral atoms generated by charge-exchange interactions between the ring current and the hydrogen geocorona can provide global images of the earth's ring current and its spatial and temporal evolution.

  12. 抗高热I号合剂对肺炎双球菌所致发热家兔脑脊液PGE、cAMP含量的影响%THE EFFECTS OF ANTI-FEVER MIXTURE I TO THE LEVELS OF PGE AND cAMP IN THE CEREBROSPINAL FLUID OF FEVER RABBITS INFECTED BY PNEUMOCOCCUS

    Institute of Scientific and Technical Information of China (English)

    蓝肇熙; 张进淘; 王杰龙; 张发荣; 谢春光; 刘宁; 孙鸿辉

    2001-01-01

    目的:观察抗高热I号合剂(AFMI)对发热家兔脑脊液前列腺素E(PGE)、环磷酸腺苷(cAMP)含量的影响。方法:用肺炎双球菌感染家兔,造成发热模型,提前应用AFMI合剂及以两种不同剂量于家兔体温达到高峰时应用,观测家兔脑脊液PGE、cAMP含量的变化并进行比较。结果:发热家兔脑脊液PGE、cAMP含量异常升高(P<0.01),AFMI可显著降低发热家兔脑脊液中异常升高的PGE和cAMP的含量(P<0.01),最佳剂量为26.25 g*kg-1, 提前应用AFMI,可阻抑感染家兔脑脊液中PGE、cAMP的合成释放。结论:AFMI具有良好的解热降温作用。%OBJECTIVE:To study effects of Anti-fever mixture I(AFMI,composed of Herba Ephedrae Decoction and Radix Puerariae Decoction) to the levels of PGE and cAMP in the cerebrospinal fluid of fever rabbits.METHODS:Rabbits were infected by pneumococcus to produce a fever model. Two different doses of AFMI were used when the rabbits' temperature reached its peak, and also used AFMI ahead of schedule. Survey the changes of PGE and cAMP levels and compared with each other.RESULTS:The levels of PGE and cAMP in fever rabbits' cerebrospinal fluid was rised abnormally(P<0.01), AFMI can lower the abnormally rised levels of PGE and cAMP in the cerebrospinal fluid(P<0.01), and the use of AFMI ahead of schedule can inhibit the synthesis and release of PGE and cAMP in the cerebrospinal fluid.CONCLUSION:The antipyretic and lowering temperature effects of AFMI are excellent.

  13. Analysis of valuable diagnostic circulating antigens in urine of rabbits infected by Schistosoma japon-icum%日本血吸虫感染兔尿液中具有诊断价值的循环抗原组分分析

    Institute of Scientific and Technical Information of China (English)

    孙成松; 汪奇志; 尹晓梅; 汪峰峰; 刘晓明; 张世清; 汪天平; 雷家慧; 李雍龙

    2013-01-01

    Objective To analyze the valuable diagnostic circulating antigens (CAg) in urine of rabbits infected by Schistosoma japonicum and provide evidence for constructing immunological diagnostic methods of urine CAg. Methods Protein antigens of urine were analyzed by SDS—PAGE. Circulating immune complexes (CIC) in urine were dissociated by glycine —hydrochloride buffer solution and then detected by Western blot based on chicken egg yolk immunoglobulin (IgY) of anti—soluble egg antigens (SEA). Results SDS—PAGE showed that there was no obvious protein band in healthy rabbit urine, but there were two main bands with the molecular weight of 146kDa and 60kDa and three secondary bands of 108kDa, 72kDa and 67kDa before dissociating CIC in infected rabbit urine. There were only 1 main band of 68kDa and 1 secondary band of 86kDa after dissociating CIC. Western blot showed the same protein band of 68kDa of infected rabbit urine after dissociating CIC had intensive reaction with the infected rabbit serum and anti—SEA IgY, but no protein band reacted with heathy rabbit serum and IgY from unimmunized egg yolk. Conclusion There were valuable diagnostic CAg in urine of rabbits infected by Schistosoma japonicum and immunological diagnostic methods of CAg could be constructed based on IgY.%目的 分析日本血吸虫感染兔尿液中具有诊断价值的循环抗原(CAg) 组分,为建立尿液CAg 免疫学诊断方法 奠定基础.方法 十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析尿液中蛋白抗原组分;甘氨酸盐酸缓冲液(简称甘盐液)解离尿液中循环免疫复合物(CIC),通过抗日本血吸虫可溶性虫卵抗原(Solube egg antigen,SEA)的鸡卵黄免疫球蛋白(IgY),采用免疫印渍技术(Westernblot)对解离后的CAg 组分进行分析.结果 SDS-PAGE 结果 显示,正常兔尿液中未见明显蛋白条带;感染兔尿液在未作CIC 解离前,有2 条主带和3 条次带,主带分子量为146kDa 和60kDa,次带分子量为108

  14. The cottontail rabbits of Virginia

    Science.gov (United States)

    Llewellyn, L.M.; Handley, C.O.

    1945-01-01

    Five races of cottontail rabbits belonging to three species occur in Virginia. One of them, the Mearns cottontail (Sylvilagus floridanus mearnsi), is reported here for the first time. It occurs in six southwestern counties of the state, while the eastern cottontail (S. f. mallurus) occurs in the remainder of the state with the exception of Smith and Fishermans islands off the eastern coast of Cape Charles, where it is replaced by Hitchens cottontail (S. f. hitchensi). The New England cottontail (S. transitionalis) is found on the higher mountain peaks, above 3000 feet, and the swamp rabbit (S. palustris) occurs in the Dismal Swamp region of southeastern Virginia.....The height of the breeding season for the eastern cottontail in Virginia is March and April, but breeding continues through the entire year except in December and January. The average litter size based on embryo counts was 4.7. The sex ratio of 234 specimens from all parts of the state, taken mostly in the December to February period, was 53 males to 47 females. That of a group of 145 rabbits live-trapped at Blacksburg during February and Marchwas 58 males to 42 females. The figures show that males are more active than females during the winter months, and therefore are more easily taken then....In transplanting cottontails from one section of the state to another, it is recommended that only cottontails of the same race as those originally present in the region being restocked be released there....Tularemia is not a common disease among rabbits in Virginia, but the rabbit ticks are often carriers of the disease and may transmit it to rabbits. Rabbit ticks are also found to be carriers of Rocky Mountain fever and American Q. fever. After the ticks drop off the rabbits to hibernate in the ground, which is likely to occur during mid-winter in Virginia, there is relatively little danger of humans contracting tularemia by contact with rabbits. Present laws in Virginia which prohibit rabbit hunting until the

  15. Selected HIV-1 Env trimeric formulations act as potent immunogens in a rabbit vaccination model

    DEFF Research Database (Denmark)

    Heyndrickx, Leo; Stewart-Jones, Guillaume; Jansson, Marianne Bendixen

    2013-01-01

    Ten to 30% of HIV-1 infected subjects develop broadly neutralizing antibodies (bNAbs) during chronic infection. We hypothesized that immunizing rabbits with viral envelope glycoproteins (Envs) from these patients may induce bNAbs, when formulated as a trimeric protein and in the presence...

  16. Experimental infection of rabbits with a recombinant bovine herpesvirus type 5 (BoHV-5 gI, gE and US9-negative Infecção experimental de coelhos com um recombinante do herpesvírus bovino tipo 5 defectivo na gI, gE e US9

    Directory of Open Access Journals (Sweden)

    Alessandra D'Avila Silva

    2009-11-01

    Full Text Available Bovine herpesvirus type 5 (BoHV-5 is a major cause of viral meningoencephalitis in cattle. The expression of different viral proteins has been associated with BoHV-5 neuropathogenesis. Among these, gI, gE and US9 have been considered essential for the production of neurological disease in infected animals. To evaluate the role of gI, gE and US9 in neurovirulence, a recombinant from which the respective genes were deleted (BoHV-5 gI-/gE-/US9- was constructed and inoculated in rabbits of two age groups (four and eight weeks-old. When the recombinant virus was inoculated through the paranasal sinuses of four weeks-old rabbits, neurological disease was observed and death was the outcome in 4 out of 13 (30.7 % animals, whereas clinical signs and death were observed in 11/13 (84.6% of rabbits infected with the parental virus. In eight weeks-old rabbits, the BoHV-5 gI-/gE-/US9- did not induce clinically apparent disease and could not be reactivated after dexamethasone administration, whereas wild type BoHV-5 caused disease in 55.5% of the animals and was reactivated. These findings reveal that the simultaneous deletion of gI, gE and US9 genes did reduce but did not completely abolish the neurovirulence of BoHV-5 in rabbits, indicating that other viral genes may also play a role in the induction of neurological disease.O herpesvírus bovino tipo 5 é uma das principais causas de meningoencefalite viral em bovinos. A expressão de diferentes proteínas virais tem sido associada à neuropatogenia do BoHV-5. Entre estas, a gI, gE e US9 têm sido consideradas essenciais para a indução de sinais neurológicos nos animais infectados. Para avaliar o papel das proteínas gI, gE e US9 na neurovirulência, construiu-se um recombinante no qual os genes que codificam estas proteínas foram deletados, denominado BoHV-5 gI-/gE-/US9-. Este vírus foi inoculado em coelhos de idades diferentes (quatro e oito semanas de idade. Quando o vírus recombinante foi inoculado

  17. Pharmacokinetics of ofloxacin in serum and vitreous humor of albino and pigmented rabbits.

    Science.gov (United States)

    Perkins, R J; Liu, W; Drusano, G; Madu, A; Mayers, M; Madu, C; Miller, M H

    1995-07-01

    The purpose of this study was to evaluate the pharmacokinetics of ofloxacin in serum and vitreous humor samples from albino and pigmented rabbits by using a recently described animal model which permits robust estimation of parameter values. The drug was administered to rabbits intravenously, multiple vitreous humor and serum samples were taken from each rabbit, and the vitreous humor and serum samples were assayed by high-pressure liquid chromatography. The pharmacokinetic parameters were determined with RSTRIP, an iterative, nonlinear, weighted, least-squares regression program. Eight New Zealand White rabbits and eight Dutch Belted rabbits (split into single-dose and multiple-dose groups) were investigated in this study. The value of penetration into the vitreous humor of albino rabbits (n = 6) was 32.6% +/- 2.12%, with terminal-elimination half-life values of 3.21 and 2.39 h, respectively, for vitreous humor and serum. In pigmented rabbits after a single dose (n = 3) and with a steady-state concentration of drug in serum (n = 4), penetration values were similar, at 30.4% +/- 2.98% and 30.0% +/- 4.12%, respectively (P > 0.10). Following a single dose of ofloxacin, pigmented animals had elimination half-life values from serum and vitreous humor of 2.64 and 4.32 h, respectively. After steady state was achieved, half-life values for serum and vitreous humor were 3.12 and 6.05 h, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

  18. Synchronous and Cogged Fan Belt Performance Assessment

    Energy Technology Data Exchange (ETDEWEB)

    Cutler, D.; Dean, J.; Acosta, J.

    2014-02-01

    The GSA Regional GPG Team commissioned the National Renewable Energy Laboratory (NREL) to perform monitoring of cogged V-belts and synchronous belts on both a constant volume and a variable air volume fan at the Byron G. Rodgers Federal Building and U.S. Courthouse in Denver, Colorado. These motor/fan combinations were tested with their original, standard V-belts (appropriately tensioned by an operation and maintenance professional) to obtain a baseline for standard operation. They were then switched to the cogged V-belts, and finally to synchronous belts. The power consumption by the motor was normalized for both fan speed and air density changes. This was necessary to ensure that the power readings were not influenced by a change in rotational fan speed or by the power required to push denser air. Finally, energy savings and operation and maintenance savings were compiled into an economic life-cycle cost analysis of the different belt options.

  19. 1962 Satellite High Altitude Radiation Belt Database

    Science.gov (United States)

    2014-03-01

    TR-14-18 1962 Satellite High Altitude Radiation Belt Database Approved for public release; distribution is unlimited. March...the Status of the High Altitude Nuclear Explosion (HANE) Trapped Radiation Belt Database”, AFRL-VS-PS-TR- 2006-1079, Air Force Research Laboratory...Roth, B., “Blue Ribbon Panel and Support Work Assessing the Status of the High Altitude Nuclear Explosion (HANE) Trapped Radiation Belt Database

  20. The White Rabbit Project

    CERN Document Server

    Serrano, J; Cattin, M; Garcia Cota, E; Lewis, J; Moreira, P; Wlostowski, T; Gaderer, G; Loschmidt, P; Dedic, J; Bär, R; Fleck, T; Kreider, M; Prados, C; Rauch, S

    2009-01-01

    Reliable, fast and deterministic transmission of control information in a network is a need formany distributed systems. One example is timing systems, where a reference frequency is used to accurately schedule time-critical messages. TheWhite Rabbit (WR) project is a multi-laboratory and multi-company effort to bring together the best of the data transfer and timing worlds in a completely open design. It takes advantage of the latest developments for improving timing over Ethernet, such as IEEE 1588 (Precision Time Protocol) and Synchronous Ethernet. The presented approach aims for a general purpose, fieldbus-like transmission system, which provides deterministic data and timing (sub-ns accuracy and ps jitter) to around 1000 stations. It automatically compensates for fiber lengths in the order of 10 km. This paper describes the WR design goals and the specification used for the project. It goes on to describe the central component of the WR system structure - the WR switch - with theoretical considerations a...

  1. Microglia activation in a pediatric rabbit model of tuberculous meningitis

    Science.gov (United States)

    Tucker, Elizabeth W.; Pokkali, Supriya; Zhang, Zhi; DeMarco, Vincent P.; Klunk, Mariah; Smith, Elizabeth S.; Ordonez, Alvaro A.; Penet, Marie-France; Bhujwalla, Zaver; Kannan, Sujatha

    2016-01-01

    ABSTRACT Central nervous system (CNS) tuberculosis (TB) is the most severe form of extra-pulmonary TB and disproportionately affects young children where the developing brain has a unique host response. New Zealand white rabbits were infected with Mycobacterium tuberculosis via subarachnoid inoculation at postnatal day 4-8 and evaluated until 4-6 weeks post-infection. Control and infected rabbit kits were assessed for the development of neurological deficits, bacterial burden, and postmortem microbiologic and pathologic changes. The presence of meningitis and tuberculomas was demonstrated histologically and by in vivo magnetic resonance imaging (MRI). The extent of microglial activation was quantified by in vitro immunohistochemistry as well as non-invasive in vivo imaging of activated microglia/macrophages with positron emission tomography (PET). Subarachnoid infection induced characteristic leptomeningeal and perivascular inflammation and TB lesions with central necrosis, a cellular rim and numerous bacilli on pathologic examination. Meningeal and rim enhancement was visible on MRI. An intense microglial activation was noted in M. tuberculosis-infected animals in the white matter and around the TB lesions, as evidenced by a significant increase in uptake of the tracer 124I-DPA-713, which is specific for activated microglia/macrophages, and confirmed by quantification of Iba-1 immunohistochemistry. Neurobehavioral analyses demonstrated signs similar to those noted in children with delayed maturation and development of neurological deficits resulting in significantly worse composite behavior scores in M. tuberculosis-infected animals. We have established a rabbit model that mimics features of TB meningitis in young children. This model could provide a platform for evaluating novel therapies, including host-directed therapies, against TB meningitis relevant to a young child's developing brain. PMID:27935825

  2. Microglia activation in a pediatric rabbit model of tuberculous meningitis

    Directory of Open Access Journals (Sweden)

    Elizabeth W. Tucker

    2016-12-01

    Full Text Available Central nervous system (CNS tuberculosis (TB is the most severe form of extra-pulmonary TB and disproportionately affects young children where the developing brain has a unique host response. New Zealand white rabbits were infected with Mycobacterium tuberculosis via subarachnoid inoculation at postnatal day 4-8 and evaluated until 4-6 weeks post-infection. Control and infected rabbit kits were assessed for the development of neurological deficits, bacterial burden, and postmortem microbiologic and pathologic changes. The presence of meningitis and tuberculomas was demonstrated histologically and by in vivo magnetic resonance imaging (MRI. The extent of microglial activation was quantified by in vitro immunohistochemistry as well as non-invasive in vivo imaging of activated microglia/macrophages with positron emission tomography (PET. Subarachnoid infection induced characteristic leptomeningeal and perivascular inflammation and TB lesions with central necrosis, a cellular rim and numerous bacilli on pathologic examination. Meningeal and rim enhancement was visible on MRI. An intense microglial activation was noted in M. tuberculosis-infected animals in the white matter and around the TB lesions, as evidenced by a significant increase in uptake of the tracer 124I-DPA-713, which is specific for activated microglia/macrophages, and confirmed by quantification of Iba-1 immunohistochemistry. Neurobehavioral analyses demonstrated signs similar to those noted in children with delayed maturation and development of neurological deficits resulting in significantly worse composite behavior scores in M. tuberculosis-infected animals. We have established a rabbit model that mimics features of TB meningitis in young children. This model could provide a platform for evaluating novel therapies, including host-directed therapies, against TB meningitis relevant to a young child's developing brain.

  3. Beyond the Kuiper Belt Edge

    Science.gov (United States)

    Sheppard, Scott

    2013-01-01

    Of the thousands of known objects beyond Neptune, only one has a perihelion significantly beyond 50 AU, Sedna at 75 AU. Most Kuiper Belt surveys to date either did not go faint enough, did not have the required long cadence to detect very slow moving objects or covered too small of an area of sky to efficiently detect objects beyond 50 AU. The dynamical and physical properties of objects in this region offer key constraints on the formation and evolution of our solar system. In order to probe the Sedna like population of objects with moderate radii (100 km) we are conducting a deep wide-field outer solar system survey. This survey will allow us to determine if the objects beyond 50 AU are fainter than expected, if there is truly a dearth of objects, or if the Kuiper Belt continues again after some sizable gap possibly caused by a planet sized object. We will be able to examine the origin of Sedna and determine if it is unique (as once believed for Pluto) or one of a new class of object. We request one night in 2013B to recover a very interesting object that we discovered at Subaru in July 2012 and complete the sky coverage needed to constrain the Sedna-like population. This one night was awarded to us in 2012B but lost because of instrument problems.

  4. Jupiter's radiation belts and atmosphere

    Science.gov (United States)

    De Pater, I.; Dames, H. A. C.

    1979-01-01

    Maps and stripscans of the radio emission from Jupiter were made during the Pioneer 10 flyby in December 1973 at wavelengths of 6 cm, 21 cm, and 50 cm using the Westerbork telescope in the Netherlands. With this instrument the disk of the planet was resolved at 6 and 21 cm. The pictures are averaged over 15 deg of Jovian longitude. At 21 cm the stripscans clearly show the existence of a 'hot region' in the radiation belts at a System III longitude (1965.0) of 255 + or - 10 deg. Its flux is about 9% of the total nonthermal flux, and it has a volume emissivity enhanced by a factor of about 1.6 with respect to the general radiation belts. The temperature of the thermal disk at 21 cm appears to be 290 + or - 20 K. This is likely due to a high ammonia mixing ratio in the atmosphere, a factor of 4-5 larger than the expected solar value of 0.00015.

  5. Formation of Kuiper Belt Binaries

    CERN Document Server

    Goldreich, P; Sari, R; Goldreich, Peter; Lithwick, Yoram; Sari, Re'em

    2002-01-01

    It appears that at least several percent of large Kuiper belt objects are members of wide binaries. Physical collisions are too infrequent to account for their formation. Collisionless gravitational interactions are more promising. These provide two channels for binary formation. In each, the initial step is the formation of a transient binary when two large bodies penetrate each other's Hill spheres. Stabilization of a transient binary requires that it lose energy. Either dynamical friction due to small bodies or the scattering of a third large body can be responsible. Our estimates favor the former, albeit by a small margin. We predict that most objects of size comparable to those currently observed in the Kuiper belt are members of multiple systems. More specifically, we derive the probability that a large body is a member of a binary with semi-major axis of order a. The probability depends upon sigma, the total surface density, Sigma, the surface density of large bodies having radius R, and theta=10^-4, t...

  6. Transient lipopolysaccharide-induced resistance to aerosolized Bacillus anthracis in New Zealand white rabbits.

    Science.gov (United States)

    Yee, Steven B; Dyer, David N; Twenhafel, Nancy A; Pitt, M Louise M

    2013-06-01

    Previous studies have demonstrated that prior infection by various bacterial pathogens induces nonspecific resistance to subsequent infection by other gram-negative and gram-positive bacterial pathogens. In the present study, we evaluated whether underlying inflammation enhanced host resistance to inhalational Bacillus anthracis infection in New Zealand White rabbits (SPF; Bordetella- and Pasteurella-free). Accordingly, rabbits were pretreated with either the inflammagen bacterial LPS (60,000 EU/kg), a component of the outer membrane of gram-negative bacteria, or saline (vehicle). Administration of LPS resulted in brief pyrexia and a significant increase in the proinflammatory cytokine TNFα, thus confirming LPS-induced inflammation. At 24 h after LPS treatment, rabbits were exposed to aerosolized B. anthracis spores (Ames strain; approximately 300 LD50). Blood samples collected at various times after challenge were cultured. Compared with their saline-pretreated counterparts, LPS-pretreated, B. anthracis challenged rabbits exhibited delays in 2 biomarkers of B. anthracis infection-anthrax-induced pyrexia (25 h versus 66 h after challenge, respectively) and bacteremia (26 h versus 63 h, respectively)-and survived longer (41 h versus 90 h, respectively). Similar to control animals, all LPS-pretreated, B. anthracis-challenged rabbits exhibited pathology consistent with inhalational anthrax. Taken together, these results suggest that prior or underlying stimulation of the innate immune system induces transient host resistance to subsequent B. anthracis infection in SPF New Zealand white rabbits. In particular, our results emphasize the importance of using animals that are free of underlying infections to prevent confounding data in studies for inhalational anthrax characterization and medical countermeasure evaluation.

  7. The Potential Spread of Mycobacterium tuberculosis into the Environment in the Creation of Spondylitis Tuberculosis Rabbit

    Directory of Open Access Journals (Sweden)

    Ahmad Jabir Rahyussalim

    2015-01-01

    Full Text Available Direct Mycobacterium tuberculosis inoculation on rabbit vertebral body was used in rabbit spinal infection study. The potential spread of Mycobacterium tuberculosis into the environment will be observed in order to create the conditions fulfilling biosafety aspects. Two groups of six New Zealand rabbits were treatment group (n=4 and control group (n=2. The treatment group had injection of 0.1 mL (107 cfu/mL suspension of Mycobacterium tuberculosis into the vertebral body T12. They were incubated for 2 to 14 weeks. One rabbit per period of 2, 4, 6, and 14 weeks was euthanized to collect feces, urine, saliva, and tissue lesions. The control group had only feces, urine, and saliva to detect bacteria using AFB staining, culture, and PCR. Both two groups were kept in individual cages. They were put together in a large cage for 3 hours every day to interact with each other. AFB staining, culture, and radiological examination showed negative result, but in one rabbit, histopathological examination showed positive result and PCR examination in another rabbit of the treatment group. Spreading score was 1.05% and infected score was 0 (null. The procedure did not reveal the potential spread of Mycobacterium tuberculosis into the environment.

  8. Rabbit Hemorrhagic Disease Virus Variant Recombinant VP60 Protein Induces Protective Immunogenicity.

    Science.gov (United States)

    Yang, Dong-Kun; Kim, Ha-Hyun; Nah, Jin-Ju; Song, Jae-Young

    2015-11-01

    Rabbit hemorrhagic disease virus (RHDV) is highly contagious and often causes fatal disease that affects both wild and domestic rabbits of the species Oryctolagus cuniculus. A highly pathogenic RHDV variant (RHDVa) has been circulation in the Korean rabbit population since 2007 and has a devastating effect on the rabbit industry in Korea. A highly pathogenic RHDVa was isolated from naturally infected rabbits, and the gene encoding the VP60 protein was cloned into a baculovirus transfer vector and expressed in insect cells. The hemagglutination titer of the Sf-9 cell lysate infected with recombinant VP60 baculovirus was 131,072 units/50 μl and of the supernatant 4,096 units/50 μl. Guinea pigs immunized twice intramuscularly with a trial inactivated RHDVa vaccine containing recombinant VP60 contained 2,152 hemagglutination inhibition (HI) geometric mean titers. The 8-week-old white rabbits inoculated with one vaccine dose were challenged with a lethal RHDVa 21 days later and showed 100% survival rates. The recombinant VP60 protein expressed in a baculovirus system induced high HI titers in guinea pigs and rendered complete protection, which led to the development of a novel inactivated RHDVa vaccine.

  9. Teratology studies in the rabbit.

    Science.gov (United States)

    Allais, Linda; Reynaud, Lucie

    2013-01-01

    The rabbit is generally the non-rodent species or second species after the rat recommended by the regulatory authorities and is part of the package of regulatory reproductive studies for the detection of potential embryotoxic and/or teratogenic effects of pharmaceuticals, chemicals, food additives, and other compounds, including vaccines (see Chapters 1-7).Its availability, practicality in housing and in mating as well as its large size makes the rabbit the preferred choice as a non-rodent species. The study protocols are essentially similar to those established for the rat (Chapter 9), with some particularities. The study designs are well defined in guidelines and are relatively standardized between testing laboratories across the world.As for the rat, large litter sizes and extensive background data in the rabbit are valuable criteria for an optimal assessment of in utero development of the embryo or fetus and for the detection of potential external or internal fetal malformations.

  10. The Administrator's "Handy Dandy" Tool Belt

    Science.gov (United States)

    Anderson, Terry

    2012-01-01

    Every good leader needs a tool belt. Throughout the author's years of building early childhood programs, she has acquired a number of tools for her personal belt. These tools have helped her sharpen her skills in supporting teachers and staff, connecting with families, and educating children. This article focuses on those leadership skills that…

  11. Biomechanics of seat belt restraint system.

    Science.gov (United States)

    Sances, Anthony; Kumaresan, Srirangam; Herbst, Brian; Meyer, Steve; Hock, Davis

    2004-01-01

    Seat belt system restrains and protects occupants in motor vehicle crashes and any slack in seat belt system induces additional loading on occupant. Signs of belt loading are more obvious in high-speed frontal collisions with heavy occupants. However subtle changes may occur at low speeds or with low forces from occupants during rollovers. In certain cases, the seat belt webbing is twisted and loaded by the occupant. The loading of webbing induces an observable fold/crimp on the seat belt. The purpose of the study is to biomechanically evaluate the force required to produce such marks using an anthropometric physical test dummy. Two tests were conducted to determine the amount of force required to put an observable fold/crimp in a shoulder belt. A head form designed by Voight Hodgson was used to represent the neck which interacted with the belt. The force was applied with a pneumatic pull ram (central hydraulic 89182 N) and the force was measured with a 44,000 N transducer load cell (DSM-10K). Results indicate that the force of over 1,000 N produced a fold or crimp in the belt.

  12. Seat Belts on School Buses: Some Considerations.

    Science.gov (United States)

    Soule, David

    1982-01-01

    A representative of the National Highway Traffic Safety Administration weighs advantages and discusses issues associated with installing seat belts in school buses. Federal regulations and research findings are considered. A list of guideline questions for school districts planning to install seat belts is included. (PP)

  13. Intelligent Belt Conveyor Monitoring and Control

    NARCIS (Netherlands)

    Pang, Y.

    2010-01-01

    Belt conveyors have been used worldwide in continuous material transport for about 250 years. Traditional inspection and monitoring of large-scale belt conveyors focus on individual critical components and response to catastrophic system failures. To prevent operational problems caused by the lack o

  14. Parameters affecting seat belt use in Greece.

    Science.gov (United States)

    Yannis, G; Laiou, A; Vardaki, S; Papadimitriou, E; Dragomanovits, A; Kanellaidis, G

    2011-09-01

    The objective of this research is the exploration of seat belt use in Greece and particularly the identification of the parameters affecting seat belt use in Greece. A national field survey was conducted for the analytical recording of seat belt use. A binary logistic regression model was developed, and the impact of each parameter on seat belt use in Greece was quantified. Parameters included in the model concern characteristics of car occupants (gender, age and position in the car), the type of the car and the type of the road network. The data collection revealed that in Greece, the non-use of seat belt on the urban road network was higher than on the national and rural road network and young and older men use seat belts the least. The developed model showed that travelling on a national road is negative for not wearing the seat belt. Finally, the variable with the highest impact on not wearing a seat belt is being a passenger on the back seats.

  15. Radiation belt dynamics during solar minimum

    Energy Technology Data Exchange (ETDEWEB)

    Gussenhoven, M.S.; Mullen, E.G. (Geophysics Lab., Air Force Systems Command, Hanscom AFB, MA (US)); Holeman, E. (Physics Dept., Boston College, Chestnut Hill, MA (US))

    1989-12-01

    Two types of temporal variation in the radiation belts are studied using low altitude data taken onboard the DMSP F7 satellite: those associated with the solar cycle and those associated with large magnetic storm effects. Over a three-year period from 1984 to 1987 and encompassing solar minimum, the protons in the heart of the inner belt increased at a rate of approximately 6% per year. Over the same period, outer zone electron enhancements declined both in number and peak intensity. During the large magnetic storm of February 1986, following the period of peak ring current intensity, a second proton belt with energies up to 50 MeV was found at magnetic latitudes between 45{degrees} and 55{degrees}. The belt lasted for more than 100 days. The slot region between the inner and outer electron belts collapsed by the merging of the two populations and did not reform for 40 days.

  16. [Review] Mary Toft's Rabbit Tale

    OpenAIRE

    Jones, Emrys

    2013-01-01

    Originally broadcast in April 2011 and aired again by BBC Radio 4 this November, Mary Toft’s Rabbit Tale is a radio drama retelling the story of its titular fraudster’s brief notoriety. With a high-profile cast – including singer Will Young as Toft’s husband and Rupert Graves as man-midwife, John Howard – the play explores a number of issues related to the alleged rabbit births of 1726 and their impact on public discourse of the time.

  17. Protective effect of Eimeria stiedae coproantigen against hepatic coccidiosis in rabbits.

    Science.gov (United States)

    Abdel Megeed, Kadria N; Abuel Ezz, Nadia M T; Abdel-Rahman, Eman H

    2005-08-01

    Immunization of rabbits against hepatic coccidiosis was tried. The animals were immunized twice with Eimeria stiedae coproantigen in freund's adjuvant with two week intervals. The rabbits were challenged orally with sporulated E. stiedae oocysts one week post last injection. The protection was assessed by number of oocysts output, number of focal liver lesions, clinical sings and antibody response. The immunization resulted in 70% protection from infection and decline in oocysts count. High level of IgG response in immunized rabbits than control infected ones was occurred and being responsible for the recorded protection. The electrophoretic make up of the coproantigen and oocyst antigen showed different patterns of separation. Common as well as specific bands to each antigen were identified. Using the rabbit sera after 3 weeks post vaccination in immunoblot assay, immunogenic components were detected of molecular weight 155, 103, 74, 66, 44, 22KD with coproantigen and 155, 115, 57, 26 KD with oocyst antigen. While, the rabbit sera after 2 and 4 weeks post challenge reacted with oocyts antigen, in immunoblot assay, revealing immunogenic bands of molecular weight 155, 115, 57, 26, 22KD and 155, 115, 57, 25 KD respectively. Bands of 22KD and 155KD are partially responsible for eliciting host protective immune response where they were recognized by immunized sera in coproantigen and by immunized infected sera in oocyst antigen.

  18. Eimeria species in wild rabbits (Oryctolagus cuniculus) in Fars province, Iran.

    Science.gov (United States)

    Razavi, S M; Oryan, A; Rakhshandehroo, E; Moshiri, A; Mootabi Alavi, A

    2010-12-01

    This investigation was accomplished during February to November 2008. A total of 71 wild rabbits (Oryctolagus cuniculus) of about 5 to 12 months age were collected alive from different parts of Fars province, south of Iran. Faecal sampling was carried out directly from recti and the oocysts were isolated using sedimentation and floatation techniques and the sporulated oocyst were identified based on morphological and biological characteristics. All the rabbits were apparently healthy and showed no clinical symptoms. Twenty two rabbits (31.0%) were positive for infection with Eimeria and six species including Eimeria perforans (18.3%), Eimeria magna (16.9%), Eimeria media (14.1), Eimeria irresidua (11.2%), Eimeria flavescens (4.2%), and Eimeria coecicola (2.8%) were identified. Eighty six percent of the infected rabbits showed mixed infections with two or three Eimeria species. Lack of clinical signs could be due to the agro ecological and environmental conditions of rabbit habitats specifically dry and hot climatic features in recent years. In addition, immunity induced by long term exposure to low doses of oocysts shedded by the carrier animals probably have pivotal role in impairing parasitic developmental cycles and preventing acute coccidiosis.

  19. Necrotizing gastritis associated with Clostridium septicum in a rabbit.

    Science.gov (United States)

    Garcia, Jorge P; Moore, Janet; Loukopoulos, Panayiotis; Diab, Santiago S; Uzal, Francisco A

    2014-09-01

    Clostridium septicum is the causative agent of histotoxic infections, including malignant edema and braxy (necrotizing abomasitis) in several animal species. The carcass of a 2-year-old, female New Zealand white rabbit with a history of acute depression and obtundation followed by death was received at the California Animal Health and Food Safety Laboratory System (San Bernardino, California) for necropsy and diagnostic workup. No gross lesions were detected at necropsy. Microscopically, there was moderate to severe, multifocal fibrinonecrotizing, transmural gastritis with numerous intralesional Gram-positive, sporulated rods, and disseminated thrombosis of the brain, lungs, heart, and liver, with occasional intravascular rods. The rods observed within the gastric wall and thrombi in the stomach and lung were positive for C. septicum by immunohistochemical staining. However, this microorganism was not isolated from stomach content. Clostridium septicum should be included in the list of possible etiologies of gastritis in rabbits.

  20. Seat belt use law in developing countries

    Institute of Scientific and Technical Information of China (English)

    SangWanLee

    1999-01-01

    Objective:To highlight the way to successful implementation of mandantory seat belt use law in developing countries particulary where have significant increase in number or cars and subsequent increase in car occupant casualties.Methods:Literatures concerning seat belt use were reviewed and experiences of the world.Satisfactory or not,investigated.It summed up general aspects of seat belt use as well as benefits,attitude toward legislation and measures to enhance the usage.Results:Seat belt use has been proven and stood time tested as the most effective means to protect car occupants from road crash injuries.It appears to be arduous to achieve the golal of seat belt use law in developing countries. but possible via strategies appropriately leading to legislation and promotion of the belt usage.Conclusions:It is prime necessity for the government authorities to recognize the importance of seat belt use.There needs an organizational structure composed of relevant professional from both private and government sectors which is able to carry out every steps toward successful legislation and implementation:education,publicity,enforcement,evaluation and dissemination of the law's benefits.

  1. Drive Alive: Teen Seat Belt Survey Program

    Directory of Open Access Journals (Sweden)

    Loftin, Laurel

    2010-08-01

    Full Text Available Objective: To increase teen seat belt use among drivers at a rural high school by implementing the Drive Alive Pilot Program (DAPP, a theory-driven intervention built on highway safety best practices.Methods: The first component of the program was 20 observational teen seat belt surveys conducted by volunteer students in a high school parking lot over a 38-month period before and after the month-long intervention. The survey results were published in the newspaper. The second component was the use of incentives, such as gift cards, to promote teen seat belt use. The third component involved disincentives, such as increased police patrol and school policies. The fourth component was a programmatic intervention that focused on education and media coverage of the DAPP program.Results: Eleven pre-intervention surveys and nine post-intervention surveys were conducted before and after the intervention. The pre- and post-intervention seat belt usage showed significant differences (p<0.0001. The average pre-intervention seat belt usage rate was 51.2%, while the average post-intervention rate was 74.5%. This represents a percentage point increase of 23.3 in seat belt use after the DAPP intervention.Conclusion: Based on seat belt observational surveys, the DAPP was effective in increasing seat belt use among rural high school teenagers. Utilizing a theory-based program that builds on existing best practices can increase the observed seat belt usage among rural high school students. [West J Emerg Med. 2010; 11(3: 280-283.

  2. Microbiological quality of rabbit meat.

    Science.gov (United States)

    Rodríguez-Calleja, Jose M; Santos, Jesús A; Otero, Andrés; García-López, María-Luisa

    2004-05-01

    World rabbit meat production is estimated to be over 1 million tons, and Spain is the third largest producer. Although rabbit meat is marketed and consumed worldwide, information on microbiological quality is very scarce. Here, we report indicator organisms, spoilage flora, sensory quality, and some physicochemical traits of 24 h postmortem chilled rabbit carcasses and prepackaged rabbit meat stored chilled in air for 0 to 3 days at the retail level. The mean total bacterial count (4.01 +/- 0.48 log CFU/g) for carcasses dressed at a small abattoir by a manual process was significantly lower (P Pseudomonas, lactic acid bacteria, and yeasts. These microorganisms and Brochothrix thermosphacta were dominant on carcasses from the large abattoir. On prepacked hind legs (pH 6.26 +/- 0.18) stored at -1 to +1 degree C (supermarket 1), mean aerobic mesophilic count was 5.87 +/- 1.03 log CFU/g, and the major microbial groups were Pseudomonas, yeasts, lactic acid bacteria, and B. thermosphacta. On prepacked whole carcasses (pH 6.37 +/- 0.18) displayed at -1 to +5 degrees C (supermarket 2), mean aerobic mesophilic count was 6.60 +/- 1.18 and the same microbial groups were dominant. Relative Escherichia coli incidence was supermarket 2 > large abattoir > supermarket 1 > small abattoir. Overall, low numbers of coliforms, Enterobacteriaceae, psychrotrophic clostridia, coagulase-positive staphylococci, and molds were found. Sensory scores, pH values, and L-lactic acid content differentiated fresh carcasses from retail samples. Data obtained suggest that the microflora of chilled rabbit meat are different from those found on the meat of other animals.

  3. Computer-aided design of conveyor belts

    Energy Technology Data Exchange (ETDEWEB)

    Karolewski, B.; Pytel, J.

    1984-01-01

    Possibilities are discussed for using mathematical models of belt conveyors for development of computer-aided design of conveyors for coal mining. Examples of optimization tasks and methods for their solution using computerized simulation are analyzed. The analysis is illustrated by an algorithm used to design a starter for the drive system of a belt conveyor. Electromagnetic moment and starting current are used as optimization criteria. A simplified model of a belt conveyor is used. The model consists of an equation of motion with variable braking moment and variable moment of inertia. 3 references.

  4. Collisional evolution of the early asteroid belt

    Science.gov (United States)

    Gil-Hutton, Ricardo; Brunini, Adrián

    1999-04-01

    We present numerical results obtained by a simulation of the collisional process between asteroids and scattered comets from the Uranus-Neptune zone. This mechanism allows the use of single exponent incremental size distributions for the initial belt reaching a final distribution that matches the observed population very well. Since the cometary bombardment was extremely efficient removing mass from the primordial asteroid belt in a very short time, we always obtained belts with total masses less than 0.001 M ⊕ after ≈ 2×10 7 yrs. This result allows processes with an important initial mass preserving Vestas basaltic crust.

  5. RPR test for serological survey of rabbit syphilis in companion rabbits.

    Science.gov (United States)

    Saito, Kumiko; Tagawa, Masayo; Hasegawa, Atsuhiko

    2003-07-01

    Since the RPR (rapid plasma regain) test was found to be useful for the diagnosis of rabbit syphilis, serological survey by this test has been carried out in Japanese companion rabbits. A hundred virgin household rabbits kept alone and without signs and history of syphilis were examined by RPR test from April 2001 to March 2002, in Tokyo, Japan. The test was positive in 35 cases and negative in 65 cases. RPR negative rabbits should be selected for breeding to prevent the spread of rabbit syphilis in companion rabbits in Japan.

  6. A Survey on the Gastrointestinal Parasites of Rabbit and Guinea Pig in a Laboratory Animal House

    Directory of Open Access Journals (Sweden)

    Motamedi, G.,

    2014-05-01

    Full Text Available There is documented evidence that infection in laboratory animals can often influence the outcome of experiments. All infections, apparent or inapparent, are likely to increase biological variability. As a research project concerning the diversity and distribution of parasites of rabbit and guinea pig in a conventional laboratory animal house, about 87 rabbits (from 700 and 105 guinea pigs (from 1500 were selected randomly from a Research, Production & Breeding of Laboratory Animals Department. Samples were collected between 19.02.2010 and 20.05.2011. The samples and animals were examined by dissection and flotation methods. In this study only one species of nematodes (Passalorus ambiguus: 6.9%; one species of protozoa (Eimeria spp.: 21.8% in rabbits and one species of nematodes (Paraspidodera Uncinata: 24.7%; one species of protozoa (Balantidium coli: 11.4% in guinea pigs were identified. However, there was not any cestodes or trematodes identified from this group of laboratory animals.

  7. Studies on coccidia species of genus Eimeria from domestic rabbit (Oryctolagus cuniculus domesticus L.) in Jeddah, Saudi Arabia.

    Science.gov (United States)

    Toula, F H; Ramadan, H H

    1998-12-01

    Five Eimeria species were reported from domestic rabbits (Oryctolagus cuniculus domesticus L.) caught from Jeddah, Saudi Arabia. According to their prevalences, they were: Eimeria perfarans (65%), E. magna (45%), E. stiedae (25%), E. exigua (20%) and E. piriformis (10%). 90% of the examined rabbits were positive and mixed infection with two or three Eimeria species was most frequent. E. stiedae, E. piriformis and E. exigua were identified and recorded for the first time from rabbits in Saudi Arabia and are considered as new locality or geographical distribution.

  8. 30 CFR 56.14131 - Seat belts for haulage trucks.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Seat belts for haulage trucks. 56.14131 Section... Equipment Safety Devices and Maintenance Requirements § 56.14131 Seat belts for haulage trucks. (a) Seat belts shall be provided and worn in haulage trucks. (b) Seat belts shall be maintained in...

  9. The specificity of the Sabin-Feldman dye test with reference to protozoal infections.

    Science.gov (United States)

    KULASIRI, C

    1960-07-01

    Infections of Eimeria stiedae in normal and splenectomized rabbits and of Leishmania enriettii in normal rabbits and guinea-pigs and in splenectomized rabbits did not produce dye test antibodies. Guinea-pigs injected with Atoxoplasma sp. and rabbits immunized with cultures of Crithidia fasciculata were found to be negative for dye test antibodies. Mice which had been infected with Trypanosoma cruzi did not show dye test antibodies. All these animals, except one guinea-pig infected with Leishmania enriettii, were negative in the complement-fixation test for toxoplasmosis using the egg antigen. These findings are discussed in the light of previous reports.

  10. Efficacy of intravenous liposomal amphotericin B (AmBisome) against coccidioidal meningitis in rabbits.

    Science.gov (United States)

    Clemons, Karl V; Sobel, Raymond A; Williams, Paul L; Pappagianis, Demosthenes; Stevens, David A

    2002-08-01

    The efficacy of intravenously administered liposomal amphotericin B (AmBisome [AmBi]) for the treatment of experimental coccidioidal meningitis was compared with those of oral fluconazole (FLC) and intravenously administered conventional amphotericin B (AMB). Male New Zealand White rabbits were infected by intracisternal inoculation of arthroconidia of Coccidioides immitis. Starting 5 days postinfection, animals received one of the following: 5% dextrose water diluent; AMB given at 1 mg/kg of body weight; AmBi given at 7.5, 15, or 22.5 mg/kg intravenously three times per week for 3 weeks; or oral FLC given at 80 mg/kg for 19 days. One week after the cessation of therapy, all survivors were euthanatized, the numbers of CFU remaining in the spinal cord and brain were determined, and histological analyses were performed. All AmBi-, FLC-, or AMB-treated animals survived and had prolonged lengths of survival compared with those for the controls (P clinical signs of infection (e.g., weight loss, elevated temperature, and neurological abnormalities including motor abnormalities). The mean histological scores for AmBi-treated rabbits were lower than those for FLC-treated and control rabbits (P < 0.016 and 0.0005, respectively); the scores for AMB-treated animals were lower than those for the controls (P < 0.0005) but were similar to those for FLC-treated rabbits. All regimens reduced the numbers of CFU in the brain and spinal cord compared with those for the controls (P < or =0.0005). AmBi-treated animals had 3- to 11-fold lower numbers of CFU than FLC-treated rabbits and 6- to 35-fold lower numbers of CFU than AmB-treated rabbits. Three of eight animals given 15 mg of AmBi per kg had no detectable infection in either tissue, whereas other doses of AmBi or FLC cleared either the brain or the spinal cord of infection in fewer rabbits. In addition, clearance of the infection from both tissues was achieved in none of the rabbits, and neither tissue was cleared of infection in

  11. Simulation of engine auxiliary drive V-belt slip motion. Part 1. Development of belt slip model; Engine hoki V belt slip kyodo no simulation. 1. Belt slip model no kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    Kurisu, T. [Mazda Motor Corp., Hiroshima (Japan)

    1997-10-01

    V-belts are widely used for driving auxiliary components of an engine. Inadequet design of such belt system sometimes results in troubles such as belt squeak, side rubber separation and/or bottom rubber crack. However, there has been no design tools which can predict belt slip quantitatively. The author developed a motion simulation program of Auxiliary Drive V-Belt System considering belt slip. The program showed good prediction accuracy for belt slip motion. This paper describes the simulation model. 1 ref., 12 figs.

  12. 纤维蛋白凝胶复合骨形态发生蛋白和庆大霉素缓释药物对感染性骨缺损的修复%Fibrin glue/bone morphogenetic protein complex plus slow-release gentamicin for repairing infected bone defects in rabbits

    Institute of Scientific and Technical Information of China (English)

    高秋明; 刘兴炎; 董晓萍; 葛宝丰; 白孟海; 陈克明

    2005-01-01

    背景:慢性骨髓炎临床处理较为棘手,手术常需分期进行,目前尚无好的方法予以一期修复.目的:探讨将纤维蛋白凝胶(FG)作为骨形态发生蛋白(BMP)及庆大霉素的共同载体,一期修复感染性骨缺损的可行性.设计:完全随机对照实验研究.单位:解放军兰州军区兰州总医院全军创伤骨科中心.材料:实验在兰州军区兰州总医院骨科研究所完成.对象为体质量1.9~2.4kg的48只成年健康青紫兰兔,雌雄不限,购自甘肃省兰州市生物制品研究所.干预:48只青紫兰兔,制作慢性骨髓炎模型,清创后造成胫骨近侧干骺端内侧1.5 cm长半环形骨缺损,采用3种方法进行处理:A组,植入FG,BMP和庆大霉素复合物;B组,植入FG/BMP复合物,C组,作为空白对照.主要观察指标:术后观察动物一般情况,做骨培养及细菌计数,X射线拍片及组织学检查.结果:A组感染控制及骨修复均良好,感染控制率、再生骨量明显优于B组.B,C两组在感染控制率上无显著差异.C组动物骨修复差.结论:FG,BMP及庆大霉素复合物具有促进成骨及抗感染的双重作用,可用于感染性骨缺损及污染严重的开放性损伤造成的骨缺损的修复.%BACKGROUND: Chronic osteomyelitis is difficult to manage clinically, and two or more operations were commonly needed. No satisfactory method for one-stage repair has been currently available.OBJECTIVE: To examine the possibility of using fibrin glue(FG) as the common carrier for both bone morphogenetic protein(BMP) and gentamicin for one-stage repair of infected bone defects.DESIGN: A completely randomized controlled experiment.SETTING: Center of Orthopaedic Surgery, Lanzhou General Hospital of Lanzhou Area Command of of Chinese PLA.MATERIALS: The experiment was conducted using 48 healthy adult Chinchilla rabbits of either sex on normal diet with body mass of 1.9 to 2.4 kg,provided by the Institute of Biological Products, Lanzhou, Gansu Province

  13. The Compositional Structure of the Asteroid Belt

    CERN Document Server

    DeMeo, Francesca E; Walsh, Kevin J; Chapman, Clark R; Binzel, Richard P

    2015-01-01

    The past decade has brought major improvements in large-scale asteroid discovery and characterization with over half a million known asteroids and over 100,000 with some measurement of physical characterization. This explosion of data has allowed us to create a new global picture of the Main Asteroid Belt. Put in context with meteorite measurements and dynamical models, a new and more complete picture of Solar System evolution has emerged. The question has changed from "What was the original compositional gradient of the Asteroid Belt?" to "What was the original compositional gradient of small bodies across the entire Solar System?" No longer is the leading theory that two belts of planetesimals are primordial, but instead those belts were formed and sculpted through evolutionary processes after Solar System formation. This article reviews the advancements on the fronts of asteroid compositional characterization, meteorite measurements, and dynamical theories in the context of the heliocentric distribution of...

  14. Seat Belt Usage on School Buses.

    Science.gov (United States)

    Farmer, Ernest

    1985-01-01

    Studies on seat belt usage conducted under contract with governmental organizations or prepared by professional societies, state and local organizations, and transportation specialists have made significant contributions, but none has successfully resolved the issue. (MLF)

  15. Radiation Belt Storm Probe (RBSP) Mission

    Science.gov (United States)

    Sibeck, D. G.; Fox, N.; Grebowsky, J. M.; Mauk, B. H.

    2009-01-01

    Scheduled to launch in May 2012, NASA's dual spacecraft Living With a Star Radiation Belt Storm Probe mission carries the field and particle instrumentation needed to determine the processes that produce enhancements in radiation belt ion and electron fluxes, the dominant mechanisms that cause the loss of relativistic electrons, and the manner by which the ring current and other geomagnetic phenomena affect radiation belt behavior. The two spacecraft will operate in low-inclination elliptical lapping orbits around the Earth, within and immediately exterior to the Van Allen radiation belts. During course of their two year primary mission, they will cover the full range of local times, measuring both AC and DC electric and magnetic fields to 10kHz, as well as ions from 50 eV to 1 GeV and electrons with energies ranging from 50 eV to 10 MeV.

  16. Radiation Belt and Plasma Model Requirements

    Science.gov (United States)

    Barth, Janet L.

    2005-01-01

    Contents include the following: Radiation belt and plasma model environment. Environment hazards for systems and humans. Need for new models. How models are used. Model requirements. How can space weather community help?

  17. Eccentricity distribution in the main asteroid belt

    CERN Document Server

    Malhotra, Renu

    2016-01-01

    The observationally complete sample of the main belt asteroids now spans more than two orders of magnitude in size and numbers more than 64,000 (excluding collisional family members). We undertook an analysis of asteroids' eccentricities and their interpretation with simple physical models. We find that Plummer's (1916) conclusion that the asteroids' eccentricities follow a Rayleigh distribution holds for the osculating eccentricities of large asteroids, but the proper eccentricities deviate from a Rayleigh distribution: there is a deficit of eccentricities smaller than $\\sim0.1$ and an excess of larger eccentricities. We further find that the proper eccentricities do not depend significantly on asteroid size but have strong dependence on heliocentric distance: the outer asteroid belt follows a Rayleigh distribution, but the inner belt is strikingly different. Eccentricities in the inner belt can be modeled as a vector sum of a primordial eccentricity vector of random orientation and magnitude drawn from a Ra...

  18. Handbook Timing Belts Principles, Calculations, Applications

    CERN Document Server

    Perneder, Raimund

    2012-01-01

    Timing belts offer a broad range of innovative drivetrain solutions; they allow low-backlash operation in robot systems, they are widely used in automated processes and industrial handling involving highly dynamic start-up loads, they are low-maintenance solutions for continuous operation applications, and they can guarantee exact positioning at high operating speeds. Based on his years of professional experience, the author has developed concise guidelines for the dimensioning of timing belt drives and presents proven examples from the fields of power transmission, transport and linear transfer technology. He offers definitive support for dealing with and compensating for adverse operating conditions and belt damage, as well as advice on drive optimization and guidelines for the design of drivetrain details and supporting systems. All market-standard timing belts are listed as brand neutral. Readers will discover an extensive bibliography with information on the various manufacturers and their websites. This...

  19. Eccentricity distribution in the main asteroid belt

    Science.gov (United States)

    Malhotra, Renu; Wang, Xianyu

    2017-03-01

    The observationally complete sample of the main belt asteroids now spans more than two orders of magnitude in size and numbers more than 64 000 (excluding collisional family members). We undertook an analysis of asteroids' eccentricities and their interpretation with simple physical models. We find that a century old conclusion that the asteroids' eccentricities follow a Rayleigh distribution holds for the osculating eccentricities of large asteroids, but the proper eccentricities deviate from a Rayleigh distribution; there is a deficit of eccentricities smaller than ∼0.1 and an excess of larger eccentricities. We further find that the proper eccentricities do not depend significantly on asteroid size but have strong dependence on heliocentric distance; the outer asteroid belt follows a Rayleigh distribution, but the inner belt is strikingly different. Eccentricities in the inner belt can be modelled as a vector sum of a primordial eccentricity vector of random orientation and magnitude drawn from a Rayleigh distribution of parameter ∼0.06, and an excitation of random phase and magnitude ∼0.13. These results imply that when a late dynamical excitation of the asteroids occurred, it was independent of asteroid size and was stronger in the inner belt than in the outer belt. We discuss implications for the primordial asteroid belt and suggest that the observationally complete sample size of main belt asteroids is large enough that more sophisticated model-fitting of the eccentricities is warranted and could serve to test alternative theoretical models of the dynamical excitation history of asteroids and its links to the migration history of the giant planets.

  20. Jupiter's Radiation Belts: Can Pioneer 10 Survive?

    Science.gov (United States)

    Hess, W N; Birmingham, T J; Mead, G D

    1973-12-07

    Model calculations of Jupiter's electron and proton radiation belts indicate that the Galilean satellites can reduce particle fluxes in certain regions of the inner magnetosphere by as much as six orders of magnitude. Average fluxes should be reduced by a factor of 100 or more along the Pioneer 10 trajectory through the heart of Jupiter's radiation belts in early December. This may be enough to prevent serious radiation damage to the spacecraft.

  1. Drive Alive: Teen Seat Belt Survey Program

    OpenAIRE

    Burkett, Katie M.; Davidson, Steve; Cotton, Carol; Barlament, James; Loftin, Laurel; Stephens, James; Dunbar, Martin; Butterfield, Ryan

    2010-01-01

    Objective: To increase teen seat belt use among drivers at a rural high school by implementing the Drive Alive Pilot Program (DAPP), a theory-driven intervention built on highway safety best practices. Methods: The first component of the program was 20 observational teen seat belt surveys conducted by volunteer students in a high school parking lot over a 38-month period before and after the month-long intervention. The survey results were published in the newspaper. The second compo...

  2. Decay rate of the second radiation belt.

    Science.gov (United States)

    Badhwar, G D; Robbins, D E

    1996-01-01

    Variations in the Earth's trapped (Van Allen) belts produced by solar flare particle events are not well understood. Few observations of increases in particle populations have been reported. This is particularly true for effects in low Earth orbit, where manned spaceflights are conducted. This paper reports the existence of a second proton belt and it's subsequent decay as measured by a tissue-equivalent proportional counter and a particle spectrometer on five Space Shuttle flights covering an eighteen-month period. The creation of this second belt is attributed to the injection of particles from a solar particle event which occurred at 2246 UT, March 22, 1991. Comparisons with observations onboard the Russian Mir space station and other unmanned satellites are made. Shuttle measurements and data from other spacecraft are used to determine that the e-folding time of the peak of the second proton belt. It was ten months. Proton populations in the second belt returned to values of quiescent times within eighteen months. The increase in absorbed dose attributed to protons in the second belt was approximately 20%. Passive dosimeter measurements were in good agreement with this value.

  3. THE GOULD'S BELT DISTANCE SURVEY

    Directory of Open Access Journals (Sweden)

    L. Loinard

    2011-01-01

    Full Text Available Observaciones que utilizan la interferometría de muy larga línea de base (VLBI por sus siglas en ingl´es pueden proveer la posición de radiofuentes compactas con una precisión del orden de 50 micro-segundos de arco. Esto es suficiente para medir la paralaje trigonométrica y los movimientos propios de cualquier objeto localizado hasta 500 pc del Sol con una precisión mejor que unos porcientos. Por ser magnéticamente activas, las estrellas jóvenes a menudo emiten emisión radio compacta detectable usando técnicas VLBI. Aquí, mostraremos cómo observaciones VLBI ya han restringido la distancia a las regiones de formación estelar cercanas más frecuentemente estudiadas (Tauro, Ofiuco, Orión, etc. y han empezado a revelar su estructura y su cinemática interna. Luego, describiremos un gran proyecto (llamado The Gould's Belt Distance Survey diseñado para proveer una vista detallada de la formación estelar en la vecindad Solar, usando observaciones VLBI.

  4. Jupiter's magnetosphere and radiation belts

    Science.gov (United States)

    Kennel, C. F.; Coroniti, F. V.

    1979-01-01

    Radioastronomy and Pioneer data reveal the Jovian magnetosphere as a rotating magnetized source of relativistic particles and radio emission, comparable to astrophysical cosmic ray and radio sources, such as pulsars. According to Pioneer data, the magnetic field in the outer magnetosphere is radially extended into a highly time variable disk-shaped configuration which differs fundamentally from the earth's magnetosphere. The outer disk region, and the energetic particles confined in it, are modulated by Jupiter's 10 hr rotation period. The entire outer magnetosphere appears to change drastically on time scales of a few days to a week. In addition to its known modulation of the Jovian decametric radio bursts, Io was found to absorb some radiation belt particles and to accelerate others, and most importantly, to be a source of neutral atoms, and by inference, a heavy ion plasma which may significantly affect the hydrodynamic flow in the magnetosphere. Another important Pioneer finding is that the Jovian outer magnetosphere generates, or permits to escape, fluxes of relativistic electrons of such intensities that Jupiter may be regarded as the dominant source of 1 to 30 MeV cosmic ray electrons in the heliosphere.

  5. In vitro and in vivo effect of Citrus limon essential oil against sarcoptic mange in rabbits

    Science.gov (United States)

    The effect of lemon oil (Citrus limon) on Sarcoptes scabiei var. cuniculi was evaluated in vitro and in vivo. The mite samples were collected from naturally infected rabbits. The lemon oil was prepared in six concentrations by dilution with distilled water (2.5, 5, 10, 20, 50, and 100 %). In vitro a...

  6. White Rabbit Status and Prospects

    CERN Document Server

    Serrano, J; Cattin, M; van der Bij, E; Wlostowski, T; Daniluk, G; Lipinski, M; Beck, D; Hoffmann, J; Kreider, M; Prados, C; Rauch, S; Terpstra, W W; Zweig, M

    2014-01-01

    The White Rabbit (WR) project started off to provide a sequencing and synchronisation solution for the needs of CERN and GSI. Since then, many other users have adopted it to solve problems in the domain of distributed hard realtime systems. The paper discusses the current performance of WR hardware, along with present and foreseen applications. It also describes current efforts to standardise WR under IEEE 1588 and recent developments on reliability of timely data distribution, finishing with an outline of future plans.

  7. Cardiovascular physiology and diseases of the rabbit.

    Science.gov (United States)

    Pariaut, Romain

    2009-01-01

    This article reviews what is known about the diagnosis and management of cardiovascular diseases in the pet rabbit. Current knowledge is based on anecdotal reports, derived from research data using the rabbit as an animal model of human cardiovascular diseases, but most importantly canine and feline cardiology. It is likely that, as cardiovascular diseases are more often recognized, more specific information will soon become available for the treatment of the pet rabbit with cardiac disease.

  8. Typhoid Fever, Below the Belt.

    Science.gov (United States)

    Raveendran, Kamakshi Mahadevan; Viswanathan, Stalin

    2016-01-01

    Genital ulcers occur due to infective, inflammatory, malignant and drug-related causes. In tropical countries such as India, such ulcers are due to parasitic, tubercular, rickettsial and bacterial (sexually transmitted infections) aetiologies. Typhoid fever is endemic in the tropics. Except "rose spots", skin manifestations in typhoid fever are unusual, and they are missed due to pigmented skin. Patients do not often complain of genital ulcers due to shame or fear. Genital examination is not routinely performed in typhoid fever. We describe scrotal ulcers as the presenting symptom of typhoid fever, which subsided with appropriate therapy.

  9. Extensive Admixture and Selective Pressure Across the Sahel Belt.

    Science.gov (United States)

    Triska, Petr; Soares, Pedro; Patin, Etienne; Fernandes, Veronica; Cerny, Viktor; Pereira, Luisa

    2015-11-26

    Genome-wide studies of African populations have the potential to reveal powerful insights into the evolution of our species, as these diverse populations have been exposed to intense selective pressures imposed by infectious diseases, diet, and environmental factors. Within Africa, the Sahel Belt extensively overlaps the geographical center of several endemic infections such as malaria, trypanosomiasis, meningitis, and hemorrhagic fevers. We screened 2.5 million single nucleotide polymorphisms in 161 individuals from 13 Sahelian populations, which together with published data cover Western, Central, and Eastern Sahel, and include both nomadic and sedentary groups. We confirmed the role of this Belt as a main corridor for human migrations across the continent. Strong admixture was observed in both Central and Eastern Sahelian populations, with North Africans and Near Eastern/Arabians, respectively, but it was inexistent in Western Sahelian populations. Genome-wide local ancestry inference in admixed Sahelian populations revealed several candidate regions that were significantly enriched for non-autochthonous haplotypes, and many showed to be under positive selection. The DARC gene region in Arabs and Nubians was enriched for African ancestry, whereas the RAB3GAP1/LCT/MCM6 region in Oromo, the TAS2R gene family in Fulani, and the ALMS1/NAT8 in Turkana and Samburu were enriched for non-African ancestry. Signals of positive selection varied in terms of geographic amplitude. Some genomic regions were selected across the Belt, the most striking example being the malaria-related DARC gene. Others were Western-specific (oxytocin, calcium, and heart pathways), Eastern-specific (lipid pathways), or even population-restricted (TAS2R genes in Fulani, which may reflect sexual selection).

  10. Seat belts, airbags and child protection devices. [previously: Seat belts and child restraint seats.

    NARCIS (Netherlands)

    2010-01-01

    In the Netherlands, the use of seat belts results in a yearly reduction of hundreds of fatalities. Seat belts reduce the risk of fatal injury by 37 to 48%, depending on the position in the car. At 50%, the effect of child protection devices is even slightly higher. When last measured (in 2010), 97%

  11. Experimental toxoplasmosis in pregnant rabbits/ Toxoplasmose experimental em coelhas gestantes

    Directory of Open Access Journals (Sweden)

    Alvimar José da Costa

    2001-08-01

    Full Text Available The clinical, hematological, obstetrical, immunological and pathological aspects of the experimental toxoplasmosis were investigated in pregnant rabbits orally inoculated with Toxoplasma gondii oocysts. Pregnant rabbits showed to be highly sensible to the toxoplasmic infection. Abortion and death occurred in 100% of the inoculated rabbits. Fever peak was observed between 4o and 9o day pos-infection. Blood parameter showed alterations. Because the hyperacute character presented by the toxoplasmic infection, the humoral immune response was weak. The microscopic and macroscopic exams demonstrated, mainly, alterations in liver, kidney and lungs. These results suggested that pregnant rabbits, orally infected with oocysts of T. gondii are extremely susceptible to this parasite, showing severe alterations in several organs, as well as premature abortion and death.Neste ensaio foram comparados aspectos clínicos, obstétricos, imunológicos e patológicos entre coelhas gestantes inoculadas com oocistos de Toxoplasma gondii e coelhas gestantes não inoculadas. Os resultados obtidos demonstram ser a coelha gestante altamente sensível à infecção toxoplásmica. Aborto e óbito foram observados em 100% das coelhas infectadas, antes do término da gestação. Os aumentos máximos de temperatura corporal foram observados entre o quarto e o nono dia pós-inoculação (DPI. Alterações hematológicas também foram observadas. Devido ao caráter hiperagudo decorrente da infecção toxoplásmica, detectou-se resposta imune humoral nas coelhas infectadas somente no 7o DPI. Os exames anátomo-histopatológicos demonstraram, principalmente, alterações hepáticas, renais e pulmonares. Com base nos dados obtidos no estudo, comprovou-se que coelhas gestantes são extremamente sensíveis a oocistos de T. gondii, apresentando severas alterações em diversos órgãos, assim como aborto e óbito prematuro.

  12. In vitro and in vivo effect of Citrus limon essential oil against sarcoptic mange in rabbits.

    Science.gov (United States)

    Aboelhadid, S M; Mahrous, Lilian N; Hashem, Shimaa A; Abdel-Kafy, E M; Miller, Robert J

    2016-08-01

    The effect of lemon oil (Citrus limon) on Sarcoptes scabiei var. cuniculi was evaluated in vitro and in vivo. The mite samples were collected from naturally infected rabbits. The lemon oil was prepared in six concentrations by dilution with distilled water (2.5, 5, 10, 20, 50, and 100 %). In vitro application was done in five replicates for each concentration in petri dishes in the laboratory. The treated mites were observed at 1, 12, and 24 h post application (PA) for lemon oil effect. In addition, oxidative stress profile was evaluated for the treated mite. Dependent on in vitro results, 20 % lemon oil was used in vivo trial. Twenty-four naturally infected rabbits were divided into three groups of eight: 20 % lemon oil, deltamethrin, and untreated control. The infected parts of rabbits were treated topically once a week for four successive weeks. In vitro application results showed that lemon oil 10 and 20 % diluted in water caused mortality to 100 % of mites after 24 h PA. The oxidative stress profile revealed that mites treated with 20 % lemon oil had significantly (P < 0.05) higher hydrogen peroxide and malondialdehyde when compared with mites treated with deltamethrin or distilled water. In vivo application of 20 % lemon oil on naturally infected rabbits showed complete recovery from clinical signs, absence of mite in microscopic examination from the second week of treatment. In addition, productive performance was significantly better than infected untreated group. Also, the treated tissue showed stoppage of scale formation and hair growth faster than deltamethrin-treated rabbits. Consequently, lemon oil has remarkable miticidal activity in vitro and in vivo applications.

  13. Analysis of immune responses against H pylori in rabbits

    Institute of Scientific and Technical Information of China (English)

    Khademul Islam; Ibrahim Khalil; Chowdhury Rafiqul Ahsan; Mahmuda Yasmin; Jamalun Nessa

    2007-01-01

    AIM: To investigate the immunogenicity of H pylori proteins, to evaluate the production rate of anti H pylori IgG antibodies in relation to time and to demonstrate the fidelity of newly optimized in-house enzymelinked immunosorbent assay (ELISA) technique as an alternative for H pylori infection assay.METHODS: In the present study, 100 μg of formalinfixed H pylori whole cell antigens was injected into an experimental animal (New Zealand white female rabbit) intramuscularly on d 0, 16, 27 and 36. The first two doses were injected with adjuvants. On d 0,a serum sample was collected from the rabbit before immunization and this pre-immunized serum was used as a negative control for the whole study. To evaluate the immunogenic responses of the injected antigen,serum samples were collected from the rabbit at regular intervals up to d 42. The sera were analyzed using inhouse ELISA and Western blot techniques.RESULTS: The production of anti H pylori IgG antibodies in the rabbit in response to the injected antigen increased almost exponentially up to d 14 and after that it was maintained at the same level until the last day (d 42). By analyzing the immune profiles of immunized sera, 11 proteins were identified to be immunogenic,among them 2 (approximately 100 kDa and 85 kDa)were most prominent.CONCLUSION: Analysis of the immune responses against pathogenic microorganisms like H pylori is necessary for the development of various diagnostic and preventive approaches. The results of this experiment reveal that the formalin-fixed H pylori whole cell antigens injected into the rabbit are highly immunogenic. These prominent proteins (approximately 100 kDa and 85 kDa)might have higher immunogenic effects among humans infected with H pylori and some of these immunogenic proteins can be included in diagnostic approaches based on serology and also for vaccine formulation. The inhouse ELISA is a promising alternative compared to invasive techniques.

  14. Myxoma virus M156 is a specific inhibitor of rabbit PKR but contains a loss-of-function mutation in Australian virus isolates.

    Science.gov (United States)

    Peng, Chen; Haller, Sherry L; Rahman, Masmudur M; McFadden, Grant; Rothenburg, Stefan

    2016-04-05

    Myxoma virus (MYXV) is a rabbit-specific poxvirus, which is highly virulent in European rabbits. The attenuation of MYXV and the increased resistance of rabbits following the release of MYXV in Australia is one of the best-documented examples of host-pathogen coevolution. To elucidate the molecular mechanisms that contribute to the restriction of MYXV infection to rabbits and MYXV attenuation in the field, we have studied the interaction of the MYXV protein M156 with the host antiviral protein kinase R (PKR). In yeast and cell-culture transfection assays, M156 only inhibited rabbit PKR but not PKR from other tested mammalian species. Infection assays with human HeLa PKR knock-down cells, which were stably transfected with human or rabbit PKR, revealed that only human but not rabbit PKR was able to restrict MYXV infection, whereas both PKRs were able to restrict replication of a vaccinia virus (VACV) strain that lacks the PKR inhibitors E3 and K3. Inactivation of M156R led to MYXV virus attenuation in rabbit cells, which was rescued by the ectopic expression of VACV E3 and K3. We further show that a mutation in the M156 encoding gene that was identified in more than 50% of MYXV field isolates from Australia resulted in an M156 variant that lost its ability to inhibit rabbit PKR and led to virus attenuation. The species-specific inhibition of rabbit PKR by M156 and the M156 loss-of-function in Australian MYXV field isolates might thus contribute to the species specificity of MYXV and to the attenuation in the field, respectively.

  15. Anatomy, Physiology and Non-dental Disorders of the Mouth of Pet Rabbits.

    Science.gov (United States)

    Donnelly, Thomas M; Vella, David

    2016-09-01

    The first part of this review focuses on the anatomy and physiology of the rabbit mouth. Practical understanding is critical to comprehend the dynamic pathologic changes of dental disease, which is one of the most common presenting problems in rabbits. The major theories of the etiopathogenesis of dental disease are presented. The second part focuses on non-dental oral disorders, which encompass only a small incidence of stomatognathic diseases when compared with dental disease. These diseases are primarily composed of infections (treponematosis, oral papillomatosis), neoplasia (frequently involving calcified tissue proliferation), and congenital abnormalities (mandibular prognathism, absent peg teeth, supernumerary peg teeth).

  16. Flat belt continuously variable high speed drive

    Energy Technology Data Exchange (ETDEWEB)

    Kumm, E.L.

    1992-02-01

    A study was undertaken at Kumm Industries funded by DOE in the NBS/DOE Energy-Related Inventions Program starting in August 1990 to design, construct and test a novel very high speed flat belt drive. The test arrangement as shown in Figure 1 consists of a multiple belt-pulley configuration that transmits power from a low speed (2000--4000 RPM) input to a small pulley turbine'' (27,000 to 55,000 RPM) and then to the low speed output variable radius pulley (2000--5000 RPM) via a special self-active tensioner. Transmitting 25 HP to and from the turbine'' corresponds to obtaining 50 HP in one direction only in a possible turbo compounded engine application. The high speed of the turbine'' belts, i.e. 100 meters/sec. at 55,000 RPM, while transferring substantial power is a new much higher operating regime for belts. The study showed that the available belts gave overall test rig efficiencies somewhat above 80% for the higher speeds (50,000 RPM) and higher powers (corresponding to above 90% in the turbocompound application) and a significantly better efficiencies at slightly lower speeds. The tests revealed a number of improved approaches in the design of such high speed drives. It appears that there is considerable possibility for further improvement and application of such equipment.

  17. The effects of Maine's change to primary seat belt law on seat belt use and public perception and awareness.

    Science.gov (United States)

    Chaudhary, Neil K; Tison, Julie; Casanova, Tara

    2010-04-01

    Maine upgraded its seat belt law to primary enforcement on September 20, 2007. Belt observations during the day and night were conducted along with awareness surveys in state licensing offices. Both daytime and nighttime observed belt use increased in the months following implementation of the law (daytime 77-84%; night 69-81%). Although daytime belt use was generally higher, nighttime belt use showed a greater increase than daytime belt use. Awareness surveys indicated that Maine motorists were clearly aware of the law change and its consequences. Survey respondents also indicated having heard both messages about enforcement and messages encouraging belt use. Primary seat belt laws may have a stronger effect on belt use at night--when risk is higher--than during the day.

  18. Ionospheric heating for radiation-belt control

    Energy Technology Data Exchange (ETDEWEB)

    Burke, W.J.; Villalon, E.

    1990-10-01

    Pitch-angle scattering interactions of electromagnetic waves in the ELF/VLF bands with trapped electrons, as formulated by Kennel and Petschek 1, describe the dynamics of the freshly filled radiation belts flux tubes. The natural existence of a slot region with electron fluxes below the Kennel-Petschek limit requires non-local wave sources. We describe a set of planned, active experiments in which VLF radiation will be injected from ground and space based transmitters in conjunction with the CRRES satellite in the radiation belts. These experiments will measure the intensity of waves driving pitch-angle diffusion and the electron energies in gyroresonance with the waves. An ability to reduce the flux of energetic particles trapped in the radiation belts by artificial means could improve the reliability of microelectronic components on earth-observing satellites in middle-altitude orbits.

  19. Friction and Wear in Timing Belt Drives

    Directory of Open Access Journals (Sweden)

    B. Stojanovic

    2010-09-01

    Full Text Available Timing belt tooth goes into contact with a drive pulley, stretched to the maximum, because of the previous tension. When the contact begins the peak of the belt tooth makes the contact with the outer surface of the pulley teeth. The process of the teeth entering into the contact zone is accompanied with the relative sliding of their side surfaces and appropriate friction force. The normal force value is changing with the parabolic function, which also leads to the changes of the friction force. The biggest value of the normal force and of the friction force is at the tooth root. Hollow between teeth and the tip of the pulley teeth are also in contact. Occasionally, the face surface of the belt and the flange are also in contact. The friction occurs in those tribomechanical systems, also. Values of these friction forces are lower compared with the friction force, which occurs at the teeth root.

  20. Effect of Shuanghuanglian Combined with Levofloxacin on Antibiotic Resistance of Staphylococcus Aureus ATCC29213 in Rabbit Tissue Cage Infection Model%双黄连联用左氧氟沙星对兔组织笼感染模型中金黄色葡萄球菌ATCC29213耐药性的影响

    Institute of Scientific and Technical Information of China (English)

    王国俊; 叶云; 冯碧敏; 李虹

    2014-01-01

    Objective To explore effects of shuanghuanglian combined with levofloxacin on antibiotic resistance of Staphylococcus Aureus ATCC29213 to levofloxacin. Methods Tissue cage infection model with Staphylococcus aureus was established in rabbits, and the infected animals were given with levofloxacin alone ( group A ) or in combination with shuanghuanglian ( group B) for 5 days respectively. Steady-state concentration of levofloxacin in tissue cage, bacteria recovery and bacterial resistance in tissue cage infection model were studied. Results Steady-state concentration of levofloxacin in tissue cage was not significantly different between group A and group B. The recovery rate of bacteria was significantly lower in group B than in group A (20. 0% vs. 100. 0%). The minimum inhibitory concentration (MIC) was lower in group B than in group A. Conclusion Shuanghuanglian combined with levofloxacin is helpful to reduce antibiotic resistance of Staphylococcus aureus to levofloxacin, indicating that some Chinese traditional medicine combined with antibiotics can reduce antibiotic resistance.%目的探讨双黄连联合左氧氟沙星对兔组织笼感染模型中金黄色葡萄球菌ATCC29213对左氧氟沙星的耐药性的影响。方法建立兔组织笼感染模型,分别用左氧氟沙星单独处理(A组)及双黄连联合左氧氟沙星处理(B组)5 d,探讨兔组织笼感染模型中左氧氟沙星在组织液中稳态浓度及经处理后兔组织笼中细菌恢复生长的情况和细菌耐药性变化。结果两组左氧氟沙星在组织液中浓度差异无统计学意义。 B组兔组织笼内细菌恢复生长发生率(20%)明显低于A组(100.0%),而左氧氟沙星对B组兔组织笼内细菌的最低抑菌浓度(MIC)值明显低于A组。结论双黄连与左氧氟沙星联合治疗有助于减少金黄色葡萄球菌对左氧氟沙星的耐药现象,提示中药联合抗菌药物可降低细菌对抗菌药物的耐药性。

  1. Experimental Lyme disease in rabbits: spirochetes found in erythema migrans and blood.

    Science.gov (United States)

    Kornblatt, A N; Steere, A C; Brownstein, D G

    1984-01-01

    In attempts to produce experimental Lyme disease, 33 rabbits were inoculated with Lyme spirochetes by tick feeding or from tick organ homogenates or cultures. Two rabbits developed erythema chronicum migrans at the site of inoculation, in one instance 2 days after injection of a tick organ homogenate and in the other instance, 17 days after feeding of infected Ixodes dammini ticks. Spirochetes were seen in skin biopsy specimens of the second lesion with Warthin-Starry and immunoperoxidase stains. Spirochetes were also recovered from blood cultures of two additional rabbits 2 weeks post-inoculation. These findings are characteristic of early Lyme disease in humans and give additional support for the spirochetal etiology of Lyme disease. Images PMID:6480108

  2. Composite Microdiscs with a Magnetic Belt

    DEFF Research Database (Denmark)

    Knaapila, Matti; Høyer, Henrik; Helgesen, Geir

    2015-01-01

    We describe an emulsion-based preparation of patchy composite particles (diameter of 100-500 mu m) consisting of a disclike epoxy core and a belt of porous polystyrene particles (diameter of 30 mu m) with magnetite within the pores. Compared to the magnetically uniform polystyrene particles......, the spontaneous aggregation of composite particles is suppressed when dispersed into liquid, which is attributed to the increased particle size, reduced magnetic susceptibility, and the shape of the magnetic domain distribution within the particles (spherical versus a belt). When the composite particles...

  3. Ionospheric heating for radiation belt control

    Science.gov (United States)

    Burke, William J.; Villalon, Elena

    1990-10-01

    Pitch-angle scattering interactions of electromagnetic waves in the ELF/VLF bands with trapped electrons describe the dynamics of the freshly filled radiation belts flux tubes. The natural existence of a 'slot' region with electron fluxes below the Kennel-Petschek limit requires non-local wave sources. A set of planned, active experiments is described in which VLF radiation is injected from ground and space band transmitters in conjunction with the Combined Release and Radiation Effects Satellite in the radiation belts. These experiments can measure the intensity if waves driving pitch-angle diffusion and the electron energies in gyroresonance with the waves.

  4. The radiation belt of the Sun

    CERN Document Server

    Gruzinov, Andrei

    2013-01-01

    For a given solar magnetic field, the near-Sun (phase-space) density of cosmic ray electrons and positrons of energy above about 10GeV can be calculated from first principles, without any assumptions about the cosmic ray diffusion. This is because the sunlight Compton drag must be more important than diffusion. If the solar magnetic field has an appreciable dipole component, the electron/positron density should have a belt-like dent, perhaps extending to several solar radii. The belt structure appears because the quasi-bound orbits are depopulated by the sunlight Compton drag.

  5. Drive Alive: Teen Seat Belt Survey Program

    OpenAIRE

    Loftin, Laurel; Barlament, James; Cotton, Carol; Davidson, Steve M; Burkett, Katie M.; Stephens, James

    2010-01-01

    Objective: To increase teen seat belt use among drivers at a rural high school by implementing the Drive Alive Pilot Program (DAPP), a theory-driven intervention built on highway safety best practices.Methods: The first component of the program was 20 observational teen seat belt surveys conducted by volunteer students in a high school parking lot over a 38-month period before and after the month-long intervention. The survey results were published in the newspaper. The second component was t...

  6. Development of belt conveyor driving system

    Institute of Scientific and Technical Information of China (English)

    FU Jun-qing(付峻青); WANG Cong(王聪); HUO Wei(霍伟)

    2004-01-01

    A short review for the existing various driving methods for belt conveyor was given, which include the analysis and comparison about the advantages, disadvantages and suitable application range of these methods. Based on this the variable-frequency-control(VFC) method for belt conveyor drive was fully discussed with focus on its application in medium-high voltage range. The principle of Neutral Point Clamped (NPC) Three-Level Inverter using high-voltage IGBTs together with the control strategy of rotor field-oriented vector control for induction motor drive were illustrated.

  7. CALCULATION OF TENSION FORCE OF BELT CONVEYOR

    Directory of Open Access Journals (Sweden)

    Ismet Ibishi

    2012-12-01

    Full Text Available In this paper is done the explanation on tension fashion of the belt conveyor which is employed in Kosovo Energy Corporation – KEK, for coal transportation to provide electric power plant. The aim of the paper enables to recognize tension forces not to pass with deformation of belt so that this problem will damage the workingprocess. Work principle is based on initial tension and tension during working process. The fact is known that the tension starts from the carriage on the way to tension mechanization, so forces on the rope passing through pulley there has to dominate the friction coefficient. All this process is related to economy of transportationmechanism.

  8. Transgenic Mouse Bioassay: Evidence That Rabbits Are Susceptible to a Variety of Prion Isolates.

    Directory of Open Access Journals (Sweden)

    Enric Vidal

    2015-08-01

    Full Text Available Interspecies transmission of prions is a well-established phenomenon, both experimentally and under field conditions. Upon passage through new hosts, prion strains have proven their capacity to change their properties and this is a source of strain diversity which needs to be considered when assessing the potential risks associated with consumption of prion contaminated protein sources. Rabbits were considered for decades to be a prion resistant species until proven otherwise recently. To determine the extent of rabbit susceptibility to prions and to assess the effects of passage of different prion strains through this species a transgenic mouse model overexpressing rabbit PrPC was developed (TgRab. Intracerebral challenges with prion strains originating from a variety of species including field isolates (ovine SSBP/1 scrapie, Nor98- scrapie; cattle BSE, BSE-L and cervid CWD, experimental murine strains (ME7 and RML and experimentally obtained ruminant (sheepBSE and rabbit (de novo NZW strains were performed. On first passage TgRab were susceptible to the majority of prions (Cattle BSE, SheepBSE, BSE-L, de novo NZW, ME7 and RML tested with the exception of SSBP/1 scrapie, CWD and Nor98 scrapie. Furthermore, TgRab were capable of propagating strain-specific features such as differences in incubation periods, histological brain lesions, abnormal prion (PrPd deposition profiles and proteinase-K (PK resistant western blotting band patterns. Our results confirm previous studies proving that rabbits are not resistant to prion infection and show for the first time that rabbits are susceptible to PrPd originating in a number of other species. This should be taken into account when choosing protein sources to feed rabbits.

  9. A low-virulence Eimeria intestinalis isolate from rabbit (Oryctolagus cuniculus) in China: molecular identification, pathogenicity, and immunogenicity.

    Science.gov (United States)

    Shi, Tuanyuan; Bao, Guolian; Fu, Yuan; Suo, Xun; Hao, Lili

    2014-03-01

    An Eimeria intestinalis isolated from a rabbit in China was first identified by amplifying the 18S small subunit (SSU) ribosomal RNA gene. The size of the amplified fragment was 1521 bp. The 18S SSU RNA gene of the E. intestinalis isolate shared 99% sequence identity with E. intestinalis isolates from France and the Czech Republic, with 100 and 96% coverage, respectively. Then, the pathogenicity and immunogenicity of the E. intestinalis isolate were evaluated in specific pathogen free (SPF) rabbits. In the pathogenicity assay, SPF rabbits in four groups were infected with 5 × 10(3), 5 × 10(4), 5 × 10(5), and 0 sporulated oocysts, respectively. Clinical signs including diarrhoea, constipation, loss of appetite, and reduction of body weight gain were observed in rabbits inoculated with 5 × 10(4) and 5 × 10(5) oocysts. And one rabbit (25 %) inoculated with 5 × 10(5) oocysts died 15 days after the inoculation. In the immunogenicity assay, SPF rabbits in five groups (named B1, B2, B3, B4, and B5) were immunised with 5 × 10(1), 5 × 10(2), 5 × 10(3), 0, and 0 sporulated oocysts, respectively. All rabbits but the B5 group were challenged with 1 × 10(6) oocysts. After the challenge, no or slight clinical signs were seen in rabbits of the B2 and B3 groups. Compared with the control, a 69.6 and 84.5% reduction of oocyst output was observed in the B2 and B3 groups, respectively. The body weight gain of the two groups was obviously higher than that of the challenge control group. All the results show that the E. intestinalis isolate has low virulence but immunogenicity in rabbit.

  10. Pathogenesis of meningoencephalitis in rabbits by bovine herpesvirus type-5 (BHV-5

    Directory of Open Access Journals (Sweden)

    Silva Adriana M. da

    1999-01-01

    Full Text Available This article describes the main aspects of bovine herpesvirus type-5 (BHV-5 neurologic infection and disease in rabbits, a candidate animal model for studying BHV-5 neuropathogenesis. Intranasal inoculation of weanling rabbits with a Brazilian BHV-5 isolate produced neurological disease and death in 78.8% (26/33 of the animals. Neurological signs started as early as 5 days post-inoculation and lasted from 10-12 hours up to several days. Most animals evolved to a moribund state or death within 24 (69.2% to 48 hours (88.5%. Neurological disease was characterized by excitability or depression, tremors, bruxism, walking or running in circles, backward arching of the head and body, incoordination, backward and sideways falling, paddling, profound depression and death. Moderate levels of infectivity were detected in several areas of the brain, most consistently in the ventro-lateral hemisphere (in 16 out of 20 animals, anterior cerebrum (15/20, midbrain (11/20, dorso-lateral hemisphere (10/20 and pons (12/26. Infectious virus was also recovered from the olfactory bulb (9/20, medulla oblongata (10/26, cerebellum (7/20, posterior cerebrum (5/20 and trigeminal ganglia (4/20. No gross lesions were observed. Microscopic lesions were mild and consisted of non-suppurative meningitis, mononuclear perivascular cuffing and focal gliosis. These changes were observed most consistently in the ventro-lateral hemisphere and anterior cerebrum. Passive immunity partially protected rabbits from BHV-5-induced encephalitis. Rabbits born to immunized dams showed a significative delay in the onset of clinical disease and reduced morbidity and mortality rates compared to rabbits born to unvaccinated dams. These results demonstrate that BHV-5-induced neurological disease can consistently be reproduced in rabbits and point towards the use of this species as an animal model to study BHV-5 neuropathogenesis.

  11. 9 CFR 354.124 - Quarantine of diseased rabbits.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Quarantine of diseased rabbits. 354... INSPECTION AND CERTIFICATION VOLUNTARY INSPECTION OF RABBITS AND EDIBLE PRODUCTS THEREOF Inspection Procedures; Ante-Mortem Inspections § 354.124 Quarantine of diseased rabbits. If live rabbits, which...

  12. Clinical aspects of lagomorph dental anatomy: the rabbit (oryctolagus cuniculus).

    Science.gov (United States)

    Crossley, D A

    1995-12-01

    The lagomorphs most commonly encountered as pets are rabbits. There are many breeds of domestic rabbit, varying from dwarf varieties with an adult weight of under one kilogram to giants weighing 10 kg. This article provides a working knowledge of the dental anatomy and physiology of rabbits so that veterinarians can interpret clinical and radiographic findings when investigating rabbits with suspected dental disease.

  13. 14 CFR 125.211 - Seat and safety belts.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Seat and safety belts. 125.211 Section 125... Requirements § 125.211 Seat and safety belts. (a) No person may operate an airplane unless there are available... share one approved safety belt and two persons occupying a multiple lounge or divan seat may share...

  14. 49 CFR 392.16 - Use of seat belts.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Use of seat belts. 392.16 Section 392.16... VEHICLES Driving of Commercial Motor Vehicles § 392.16 Use of seat belts. A commercial motor vehicle which has a seat belt assembly installed at the driver's seat shall not be driven unless the driver...

  15. Observed seat belt use in Kumasi Metropolis, Ghana.

    Science.gov (United States)

    Afukaar, Francis K; Damsere-Derry, James; Ackaah, Williams

    2010-01-01

    We conducted an observational survey of seat belt use to determine the use rate of drivers and front-right passengers of vehicles in Kumasi, Ghana. Unobtrusive observations of seat belt use were made at 41 locations composed of signalized intersections and roundabouts where vehicles come to a halt or slow down considerably. The overall driver seat belt use rate was 17.6% compared to 4.9% for front-right passengers. Driver belt use was 33.2% for private cars, 9.0% for taxis, 8.3% for minibus (trotro), 13.1% for large buses and 9.7% for trucks. Overall seat belt use was higher for female drivers than for male drivers (44.8% versus 16.4%, p seat belt use rate increased with age. Passengers belted more often if drivers were belted, but about three-quarters of male passengers and 70-80% of female passengers were unbelted even when drivers were belted. In conclusion, the seat belt use rate was generally low in Kumasi, Ghana, and it is a function of occupant seating position, gender, vehicle type and usage, age group, and location setting. The results provide important preliminary data about seat belt use, particularly among male drivers and commercial vehicle occupant population. The study also suggests the need to develop effective strategies and programs that address low seat belt use in Ghana.

  16. The thrust belts of Western North America

    Energy Technology Data Exchange (ETDEWEB)

    Moulton, F.C.

    1993-08-01

    Most of the Basin and Range physiographic province of western North America is now believed to be part of the overthrust. The more obvious overthrust belt along the eastern edge of the Basin and Range Province is named the Sevier orogenic belt, where older rocks are observed thrust onto younger rocks. More detailed surface geological mapping, plus deep multiple-fold geophysical work and many oil and gas wildcat wells, have confirmed an east-vergent shortened and stacked sequence is present in many places in the Basin and Range. This western compressive deformed area in east central Nevada is now named the Elko orogenic belt by the U.S. Geological Survey. This older compressed Elko orogenic belt started forming approximately 250 m.y. ago when the North American plate started to move west as the Pangaea supercontinent started to fragment. The North American plate moved west under the sediments of the Miogeocline that were also moving west. Surface-formed highlands and oceanic island arcs on the west edge of the North American plate restricted the westward movement of the sediments in the Miogeocline, causing east-vergent ramp thrusts to form above the westward-moving North American plate. The flat, eastward-up-cutting thrust assemblages moved on the detachment surfaces.

  17. School Buses & Seat Belts: A Discussion.

    Science.gov (United States)

    Alaska State Dept. of Education, Juneau.

    Safety belts are not installed in school buses for several reasons. School buses are constructed differently from automobiles in terms of (1) the locations of doors and instrument panels relative to passengers, (2) outer construction, (3) seat design and padding, and (4) visibility on the road. Under current regulations, bus seats are constructed…

  18. Design aspects of multiple driven belt conveyors

    NARCIS (Netherlands)

    Nuttall, A.J.G.

    2007-01-01

    Worldwide belt conveyors are used to transport a great variety of bulk solid materials. The desire to carry higher tonnages over longer distances and more diverse routes, while keeping exploitation costs as low as possible, has fuelled many technological advances. An interesting development in the r

  19. Belts and Chains. FOS: Fundamentals of Service.

    Science.gov (United States)

    John Deere Co., Moline, IL.

    This manual on belts and chain drives is one of a series of power mechanics texts and visual aids on theory, of operation, diagnosis, and repair of automotive and off-the-road agricultural and construction equipment. Materials provide basic information and illustrations for use by vocational students and teachers as well as shop servicemen and…

  20. Welfare aspects in rabbit rearing and transport

    Directory of Open Access Journals (Sweden)

    Claudio Cavani

    2010-01-01

    Full Text Available The review starts with the description of the rabbits’ (Oryctolagus cuniculus main habits and the current situation concerning the rabbit husbandry and management systems, as well as their effects on the welfare of these animals. As far as the intensive rabbit husbandry systems are concerned, the main problems are related to the time since rabbits have been domesticated and their adaptive capacity and coping styles as respects the farming environment and management systems. Both these aspects have implications in the present and future of rabbit rearing for different purposes. Examples are given on the effects of different housing and management systems on rabbit welfare, as well as examples of the ethological, physiological and productive indicators used to evaluate these effects. Transportation and, more generally, preslaughter phases including catching, fasting and lairage at the abattoir are considered major stressors for farmed rabbits and might have deleterious effects on health, well-being, performance, and finally, product quality. A general statement of the recent scientific studies considering the effects of pre-slaughter factors on physiological and productive measurements are reported. Finally, some indications in order to improve rabbit welfare, already present at the European level, are also outlined, together with the European Food Safety Authority opinions.

  1. Imaging Jupiter Radiation Belts At Low Frequencies

    Science.gov (United States)

    Girard, J. N.; de Pater, I.; Zarka, P.; Santos-Costa, D.; Sault, R.; Hess, S.; Cecconi, B.; Fender, R.; Pewg, Lofar

    2014-04-01

    The ultra-relativistic electrons, trapped in the inner radiation belts of Jupiter, generates a strong synchrotron radio emission (historically known as the jovian decimeter radiation (DIM)) which is beamed, polarized (~20% linear, ~1% circular) and broadband. It has been extensively observed by radio telescopes/ probes and imaged by radio interferometers over a wide frequency spectrum (from >300 MHz up to 22 GHz). This extended emission presents two main emission peaks constantly located on both sides of the planet close to the magnetic plane. High latitude emissions were also regularly observed at particular frequencies, times and in particular observational configurations. This region of the magnetosphere is "frozen" due to the strong magnetic field (~4.2 G as the equator) and therefore is forced to rotate at the planetary period (T≈9h55m). Due to the tilt (~ 10o) between the spin axis of the planet and the magnetic axis (which can be seen as dipolar in first approximation), the belts and the associated radio emission wobble around the planet center. The analysis of the flux at different frequencies highlighted spatial, temporal and spectral variabilities which origins are now partly understood. The emission varies at different time scales (short-time variations of hours to long-term variation over decades) due to the combination of visibility effect (wobbling, beaming, position of the observer in the magnetic rotating reference frame) [1], [2] and intrinsic local variations (interaction between relativistic electrons and satellites/dust, delayed effect of the solar wind ram pressure, impacts events) [3], [4], [5]. A complete framework is necessary to fully understand the source, loss and transport processes of the electrons originating from outside the belt, migrating by inward diffusion and populating the inner region of the magnetosphere. Only a few and unresolved measurements were made below 300 MHz and the nonsystematic observation of this radio emission

  2. Newer antipsychotics and the rabbit syndrome

    Directory of Open Access Journals (Sweden)

    Masalehdan Azadeh

    2007-06-01

    Full Text Available Abstract Background Rabbit syndrome is a movement disorder that is associated with long-term exposure to neuroleptic medications. Of particular interest and importance is the risk of rabbit syndrome with exposure to the newer atypical antipsychotics. Our recent experience with such a case brought to light the importance of exploring this risk. Methods MEDLINE and PubMed (1972–2006 databases were searched for English language articles using the keywords rabbit syndrome, tardive dyskinesia, antipsychotic, extrapyramidal symptoms and side effects. A recent case study is used to expand upon the literature available on newer antipsychotics and rabbit syndrome. Results We reviewed papers that addressed the following aspects of rabbit syndrome 1 the clinical manifestations 2 prevalence and risk factors, 3 etiopathogenesis 4 older antipsychotics and rabbit syndrome 5 newer antipsychotics, 6 treatment options. Moreover, we report a case of RS in a 50 year old white female, diagnosed with bipolar I disorder, that, after the discontinuation of risperidone, developed involuntary movements of the mouth that were fine, rhythmic and rapid, along the vertical axis, and without involvement of the tongue. After the re-introduction of risperidone, the symptoms decreased in a few hours and disappeared after 3 days. Conclusion Eleven cases of rabbit syndrome have been documented since the implementation of newer antipsychotics. Future research is needed to better understand the etiopathogenesis of rabbit syndrome in psychiatric populations treated with the atypical antipsychotics. Understanding the differences and similarities of rabbit syndrome and tardive dyskinesia is crucial to the creation of a successful treatment paradigm.

  3. VERO cells harbor a poly-ADP-ribose belt partnering their epithelial adhesion belt

    Directory of Open Access Journals (Sweden)

    Laura Lafon-Hughes

    2014-10-01

    Full Text Available Poly-ADP-ribose (PAR is a polymer of up to 400 ADP-ribose units synthesized by poly-ADP-ribose-polymerases (PARPs and degraded by poly-ADP-ribose-glycohydrolase (PARG. Nuclear PAR modulates chromatin compaction, affecting nuclear functions (gene expression, DNA repair. Diverse defined PARP cytoplasmic allocation patterns contrast with the yet still imprecise PAR distribution and still unclear functions. Based on previous evidence from other models, we hypothesized that PAR could be present in epithelial cells where cadherin-based adherens junctions are linked with the actin cytoskeleton (constituting the adhesion belt. In the present work, we have examined through immunofluorescence and confocal microscopy, the subcellular localization of PAR in an epithelial monkey kidney cell line (VERO. PAR was distinguished colocalizing with actin and vinculin in the epithelial belt, a location that has not been previously reported. Actin filaments disruption with cytochalasin D was paralleled by PAR belt disruption. Conversely, PARP inhibitors 3-aminobenzamide, PJ34 or XAV 939, affected PAR belt synthesis, actin distribution, cell shape and adhesion. Extracellular calcium chelation displayed similar effects. Our results demonstrate the existence of PAR in a novel subcellular localization. An initial interpretation of all the available evidence points towards TNKS-1 as the most probable PAR belt architect, although TNKS-2 involvement cannot be discarded. Forthcoming research will test this hypothesis as well as explore the existence of the PAR belt in other epithelial cells and deepen into its functional implications.

  4. China belting up or down? Seat belt wearing trends in Nanjing and Zhoushan.

    Science.gov (United States)

    Routley, Virginia; Ozanne-Smith, Joan; Li, Dan; Yu, Min; Wang, Jianyue; Zhang, Junhe; Tong, Zhendong; Wu, Ming; Wang, Peihua; Qin, Yu

    2008-11-01

    National seat belt wearing legislation became effective in China May 2004 and associated provincial and city regulations followed. Despite rapid motorisation seat belt studies in China have been scarce. Patterns and trends in urban seat belt wearing were observed for all driver, front and rear seating positions over the years 2005-2007 in two eastern cities Nanjing (Jiangsu Province) and Zhoushan (Zhejiang Province). There were 35,256 vehicles observed in Nanjing, 20,939 in Zhoushan and 95,933 occupants overall. Males dominated all seating positions, especially drivers. Seat belt wearing overall was significantly higher for drivers (49.9% Nanjing, 47.4% Zhoushan) than for front seat passengers (9.1% Nanjing, 1.0% Zhoushan) and virtually nonexistent for rear passengers (0.5% Nanjing, 0.2% Zhoushan). Generally levels declined significantly from year to year (drivers Nanjing 66.7%, 47.7%, 38.6%; Zhoushan 57.4%, 57.9%, 30.6%; front passengers Nanjing 19.2%, 6.6%, 3.2%). Zhoushan wearing did not initially decline, 2006 observations coinciding with anticipation of provincial regulations (July 2006). Observations revealed an absence of child restraints. Pretend wearing/belt tampering was observed almost exclusively in taxi drivers (14.2% of Nanjing taxi drivers, 11.3% of Zhoushan's). Awareness of and attitudes to urban seat belt laws should be investigated, appropriate countermeasures developed and enforcement reassessed.

  5. The Gould Belt, the de Vaucouleurs-Dolidze Belt, and the Orion Arm

    CERN Document Server

    Bobylev, V V

    2014-01-01

    Based on masers with measured trigonometric parallaxes, we have redetermined the spatial orientation parameters of the Local (Orion) arm. Using 23 sources (the Gould Belt objects were excluded), we have found that their spatial distribution can be approximated by a very narrow ellipsoid elongated in the direction L_1=77.1+/-2.9^o whose symmetry plane is inclined to the Galactic plane at an angle of 5.6+/-0.2^o. The longitude of the ascending node of the symmetry plane is l_\\Omega=70+/-3^o. A new estimate for the pitch angle of the Local spiral arm has been obtained by an independent method: i=12.9+/-2.9^o. Previously, a belt of young B stars, the de Vaucouleurs.Dolidze belt, was pointed out on the celestial sphere with parameters close to such an orientation. We have refined the spatial orientation parameters of this belt based on a homogeneous sample of protostars. The de Vaucouleurs.Dolidze belt can be identified with the Local arm, with the belt proper as a continuous band on the celestial sphere like the ...

  6. Bobcat attack on a cottontail rabbit

    Science.gov (United States)

    Biggins, D.E.; Biggins, D.M.

    2006-01-01

    We observed an attack by a bobcat (Lynx rufus) on a cottontail rabbit (Sylvilagus) that involved stealthy approach by the cat for >1 h, followed by a 12.3-s chase covering 116.0 m for the cat and 128.4 m for the rabbit. During the chase, the route of the cat from starting point to kill site was more direct than the semi-circular route of the rabbit. Stride lengths for the cat and total distance covered by the chase were longer than those previously reported for bobcats.

  7. Ultrastructural Comparison of the Nasal Epithelia of Healthy and Naturally Affected Rabbits with Pasteurella multocida A

    Directory of Open Access Journals (Sweden)

    Paula Esquinas

    2013-01-01

    Full Text Available An ultrastructural comparison between the nasal cavities of healthy rabbits and those suffering from two forms of spontaneous infection with Pasteurella multocida was undertaken. Twelve commercially produced rabbits of different ages and respiratory health status were divided into four groups: healthy from 0 to 21 days (G1, n=2; healthy from 23 to 49 days (G2, n=2; healthy from 51 to 69 days (G3, n=2; diseased rabbits with septicemia and the rhinitic form of P. multocida infection (G4, n=3. The main ultrastructural changes observed were a widening of the interepithelial spaces, increased activity and number of goblet cells, the formation of two types of vacuoles in epithelial cells, the degranulation and migration of heterophils between the epithelial cells, and the association of this migration with some of the other changes. No bacteria were observed adhering to the epithelium, and very few were observed free in the mucus. Scant inter-epithelial spaces were found in healthy rabbits, but they were not as large and numerous as those found in diseased animals. We discuss the origin and meaning of these changes but, we focus on the significance of the inter-epithelial spaces and goblet cells for the defense of the upper respiratory airways against the bacterium and its lipopolysaccharide.

  8. Cryptosporidium cuniculus and Giardia duodenalis in rabbits: genetic diversity and possible zoonotic transmission.

    Directory of Open Access Journals (Sweden)

    Weizhe Zhang

    Full Text Available BACKGROUND: Cryptosporidium and Giardia are the two important zoonotic pathogens causing diarrhea of humans and animals worldwide. Considering the human cryptosporidiosis outbreak and sporadic cases caused by C. cuniculus, the important public health significance of G. duodenalis and little obtained information regarding rabbit infected with Cryptosporidium and Giardia in China, the aim of this study is to determine the prevalence and molecularly characterize Cryptosporidium and Giardia in rabbits in Heilongjiang Province, China. METHODOLOGY/PRINCIPAL FINDINGS: 378 fecal samples were obtained from rabbits in Heilongjiang Province. Cryptosporidium oocysts and Giardia cysts were detected using Sheather's sugar flotation technique and Lugol's iodine stain method, respectively. The infection rates of Cryptosporidium and Giardia were 2.38% (9/378 and 7.41% (28/378, respectively. Genotyping of Cryptosporidium spp. was done by DNA sequencing of the small subunit rRNA (SSU rRNA gene and all the nine isolates were identified as Cryptosporidium cuniculus. The nine isolates were further subtyped using the 60-kDa glycoprotein (gp60 gene and two subtypes were detected, including VbA32 (n = 3 and a new subtype VbA21 (n = 6. G. duodenalis genotypes and subtypes were identified by sequence analysis of the triosephosphate isomerase (TPI gene. The assemblage B (belonging to eight different subtypes B-I to B-VIII was found in 28 G. duodenalis-positive samples. CONCLUSIONS/SIGNIFICANCE: The rabbits have been infected with Cryptosporidium and Giardia in Heilongjiang Province. The results show that the rabbits pose a threat to human health in the studied areas. Genotypes and subgenotypes of C. cuniculus and G. duodenalis in this study might present the endemic genetic characterization of population structure of the two parasites.

  9. Pseudogenization of the MCP-2/CCL8 chemokine gene in European rabbit (genus Oryctolagus, but not in species of Cottontail rabbit (Sylvilagus and Hare (Lepus

    Directory of Open Access Journals (Sweden)

    van der Loo Wessel

    2012-08-01

    Full Text Available Abstract Background Recent studies in human have highlighted the importance of the monocyte chemotactic proteins (MCP in leukocyte trafficking and their effects in inflammatory processes, tumor progression, and HIV-1 infection. In European rabbit (Oryctolagus cuniculus one of the prime MCP targets, the chemokine receptor CCR5 underwent a unique structural alteration. Until now, no homologue of MCP-2/CCL8a, MCP-3/CCL7 or MCP-4/CCL13 genes have been reported for this species. This is interesting, because at least the first two genes are expressed in most, if not all, mammals studied, and appear to be implicated in a variety of important chemokine ligand-receptor interactions. By assessing the Rabbit Whole Genome Sequence (WGS data we have searched for orthologs of the mammalian genes of the MCP-Eotaxin cluster. Results We have localized the orthologs of these chemokine genes in the genome of European rabbit and compared them to those of leporid genera which do (i.e. Oryctolagus and Bunolagus or do not share the CCR5 alteration with European rabbit (i.e. Lepus and Sylvilagus. Of the Rabbit orthologs of the CCL8, CCL7, and CCL13 genes only the last two were potentially functional, although showing some structural anomalies at the protein level. The ortholog of MCP-2/CCL8 appeared to be pseudogenized by deleterious nucleotide substitutions affecting exon1 and exon2. By analyzing both genomic and cDNA products, these studies were extended to wild specimens of four genera of the Leporidae family: Oryctolagus, Bunolagus, Lepus, and Sylvilagus. It appeared that the anomalies of the MCP-3/CCL7 and MCP-4/CCL13 proteins are shared among the different species of leporids. In contrast, whereas MCP-2/CCL8 was pseudogenized in every studied specimen of the Oryctolagus - Bunolagus lineage, this gene was intact in species of the Lepus - Sylvilagus lineage, and was, at least in Lepus, correctly transcribed. Conclusion The biological function of a gene was often

  10. 蛇毒抗菌肽OH-CATH对大肠杆菌引起家兔泌尿系感染的保护作用%Protective effects of snake venom antimicrobial peptide OH-CATH on E.coli induced rabbit urinary tract infection models

    Institute of Scientific and Technical Information of China (English)

    张白羽; 李思熳; 高振华; 申吉泓

    2013-01-01

    为探讨蛇毒抗菌肽OH-CATH对大肠杆菌标准株和临床耐药株引起家兔泌尿系感染的保护作用,该文利用大肠杆菌标准株(E.coli ATCC 25922)和耐头孢菌素大肠杆菌临床耐药株建立雄性家兔泌尿系感染模型.通过膀胱直接给药的方式,分别给予动物感染模型生理盐水、蛇毒抗菌肽OH-CATH及头孢哌酮舒巴坦,并于给药后第1、5、l0及14天留取家兔中段尿用于尿液培养.将动物膀胱标本行H-E染色石蜡切片及透射电镜观察.结果显示:使用蛇毒抗菌肽OH-CATH的大肠杆菌标准株和临床耐药株不同组别中段尿培养阳性率明显降低;给予头孢哌酮舒巴坦可降低大肠杆菌标准株(E.coli ATCC 25922)引起感染的阳性率,但对耐头孢菌素大肠杆菌引起的感染的阳性率则无明显作用(P<0.05);使用蛇毒抗菌肽OH-CATH组炎症细胞浸润、坏死及钙化组织较其他组为少.该结果提示蛇毒抗菌肽OH-CATH对大肠杆菌标准株(E.coli ATCC 25922)及耐头孢菌素大肠埃希菌临床耐药株有稳定活性,对其引起的家兔泌尿系感染有较好的保护作用,并为临床日益严重的耐药病原菌感染的治疗提供了新的思路和方向.%To investigate the potential protective effects of the snake venom antimicrobial peptide OH-CATH, we used a series of rabbit urinary tract infection models successfully induced by cephalosporin-resistant E.coli and E. coli ATCC 25922. The experimental models were administered saline, snake venom antimicrobial peptide OH-CATH, Cefoperazone and Sulbactam through the urethra. Urine was collected on days 1,5, 10 and 14 after model establishment and urine culture was done to check the infection in each experimental animals. On day 14, all the animals were sacrificed and the bladder tissue specimens were taken for observation by H-E staining light microscope and transmission electron microscope. We found that the snake venom antimicrobial peptide OH

  11. [Species from genus Eimeria observed in domestic rabbit (Oryctolagus cuniculus) feces raised at the Municipality of Campos dos Goytacazes in the State of Rio de Janeiro, Brazil].

    Science.gov (United States)

    de Almeida, Adriana J; Mayen, Friederike L; de Oliveira, Francisco C R

    2006-01-01

    This study was designed to identify species of the genus Eimeria from eleven rabbit meat breeders from Campos dos Goytacazes. Fecal samples were collected from rabbits with different health conditions and consintence of feces. The following species of Eimeria were identified: E. perforans; E. magna; E. coecicola; E. irresidua; E. media; E. flavescens; E. nagpurensis; E. intestinalis, E. exigua and E. stiedae. Parasites from this genus were detected in 81.82% (9) of the rabbit meat production sites, regardless of the management and hygiene conditions. In all cases the infection was always caused from more than one species of Eimeria.

  12. Strategies to Address Infection Prevention and Treatment in the Reduced Inflammatory Milieu of Irrigated Open Wound

    Science.gov (United States)

    2012-10-01

    infections. A member of our institutional IACUC is a veterinarian with substantial experience with rabbits as experimental models, suggested that it was a...stabilizer). • Intestinal blockage caused fatalities in rabbits. With consultation of IACUC and veterinarian researchers, room temperature will...RR, Laird CW: Diurnal variations in rabbits: Hematological parameters. Am J Physiol 218:1609, 1970. 13. Laird CW, Fox RR, Mitchell BP, et al: Effect

  13. Seat Belt Use Among Adult Workers - 21 States, 2013.

    Science.gov (United States)

    Boal, Winifred L; Li, Jia; Rodriguez-Acosta, Rosa L

    2016-06-17

    Roadway incidents involving motorized vehicles accounted for 24% of fatal occupational injuries in the United States during 2013 and were the leading cause of fatal injuries among workers.* In 2013, workers' compensation costs for serious, nonfatal injuries among work-related roadway incidents involving motorized land vehicles were estimated at $2.96 billion.(†) Seat belt use is a proven method to reduce injuries to motor vehicle occupants (1). Use of lap/shoulder seat belts reduces the risk for fatal injuries to front seat occupants of cars by 45% and the risk to light truck occupants by 60%.(§) To characterize seat belt use among adult workers by occupational group, CDC analyzed data from the 2013 Behavioral Risk Factor Surveillance System (BRFSS) and found that not always using a seat belt was significantly associated with occupational group after controlling for factors known to influence seat belt use. Occupational groups with the highest prevalences of not always using a seat belt included construction and extraction; farming, fishing, and forestry; and installation, maintenance, and repair. To increase seat belt use among persons currently employed, states can enact and enforce primary seat belt laws, employers can set and enforce safety policies requiring seat belt use by all vehicle occupants, and seat belt safety advocates can target interventions to workers in occupational groups with lower reported seat belt use.

  14. LOAD DISTRIBUTION ON DRUMS OF DOUBLE DRIVE BELT CONVEYOR

    Institute of Scientific and Technical Information of China (English)

    薛河; 苏清祖; 马胜利

    1999-01-01

    The double drum drive is widely used on the mine belt conveyor, which are divided the rigid connected double drums and separately driving double drums according to connected method of two drums. Because of the change of real work condition, the load distribution is changed on the two drive drums, which may produce a slippage between a drum and belt. Slippage may intensify the wear of the drum, and sometimes causing the fire of the belt. This restricts the development toward narrow belt, high velocity and large power of belt conveyor. In this paper, the factors affecting the load distribution of two drums of double drum belt conveyor are'systematically analyzed and some computing formulas derived, by these formulas, the actual load distribution onthe two drums of rigid connected or separately driving belt conveyor can be separately calculated. These formulas also can be as the theory base for adjusting the driving force of two drums.

  15. Phosphodiesterase-4 inhibition alters gene expression and improves isoniazid-mediated clearance of Mycobacterium tuberculosis in rabbit lungs.

    Directory of Open Access Journals (Sweden)

    Selvakumar Subbian

    2011-09-01

    Full Text Available Tuberculosis (TB treatment is hampered by the long duration of antibiotic therapy required to achieve cure. This indolent response has been partly attributed to the ability of subpopulations of less metabolically active Mycobacterium tuberculosis (Mtb to withstand killing by current anti-TB drugs. We have used immune modulation with a phosphodiesterase-4 (PDE4 inhibitor, CC-3052, that reduces tumor necrosis factor alpha (TNF-α production by increasing intracellular cAMP in macrophages, to examine the crosstalk between host and pathogen in rabbits with pulmonary TB during treatment with isoniazid (INH. Based on DNA microarray, changes in host gene expression during CC-3052 treatment of Mtb infected rabbits support a link between PDE4 inhibition and specific down-regulation of the innate immune response. The overall pattern of host gene expression in the lungs of infected rabbits treated with CC-3052, compared to untreated rabbits, was similar to that described in vitro in resting Mtb infected macrophages, suggesting suboptimal macrophage activation. These alterations in host immunity were associated with corresponding down-regulation of a number of Mtb genes that have been associated with a metabolic shift towards dormancy. Moreover, treatment with CC-3052 and INH resulted in reduced expression of those genes associated with the bacterial response to INH. Importantly, CC-3052 treatment of infected rabbits was associated with reduced ability of Mtb to withstand INH killing, shown by improved bacillary clearance, from the lungs of co-treated animals compared to rabbits treated with INH alone. The results of our study suggest that changes in Mtb gene expression, in response to changes in the host immune response, can alter the responsiveness of the bacteria to antimicrobial agents. These findings provide a basis for exploring the potential use of adjunctive immune modulation with PDE4 inhibitors to enhance the efficacy of existing anti-TB treatment.

  16. Effect of pulmonary surfactant on the expression of TGF-β1 in fetus lung injury in rabbit model with intrauterine infection%肺表面活性物质对肺损伤胎兔肺组织转化生长因子β1表达的影响

    Institute of Scientific and Technical Information of China (English)

    吴静; 刘冬云; 刘敬; 封志纯

    2012-01-01

    Objective:To investigate the effect of Intra-amniotic administration of pulmonary surfactant on the expression of TGF-β1 in fetus lung injury in rabbit model with intrauterine infection. Methods: Nineteen Japanese albino pregnant rabbits were divided into 3 groups%目的:探讨羊膜腔内注射肺表面活性物质(PS)对宫内感染致肺损伤胎兔的肺组织转化生长因子β1( TGF-β1)表达的影响.方法:选取健康成年育龄日本大耳白兔19只,成功受孕后随机分为对照组、细菌组、细菌+ PS组,建立宫内感染模型.对照组和细菌组按胎龄又分为21、22、23、24、26 d5个亚组,细菌+ PS组分为24、26 d2个亚组.细菌组宫内注射E.coli菌株,细菌+PS组宫内注射E.coli菌株的同时羊膜腔内注射PS200 mg/kg,对照组宫内注射生理盐水.模型建成后分别在各时相点取胎兔肺组织,免疫组织化学法检测肺组织内TGF-β1表达;RT-PCR检测TGF-β1mRNA表达;Western blotting法检测TGF-β1蛋白表达.结果:对照组TGF-β1蛋白在支气管上皮或血管内皮细胞及其周围的结缔组织有阳性表达,细菌组、细菌+ PS组TGF-β1阳性蛋白表达量增加,与对照组比较,差异有统计学意义(P<0.05).TGF-β1mRNA、蛋白表达在细菌感染后明显上调,3组比较,差异有显著统计学意义(F=631.109、183.980,P=0.000).结论:宫内感染后胎肺TGF-β1高表达是肺损伤的高危因素.羊膜腔内注射PS可使TGF-β1表达下降,对减轻肺损伤、促进肺发育具有积极作用.

  17. A Century of Shope Papillomavirus in Museum Rabbit Specimens.

    Science.gov (United States)

    Escudero Duch, Clara; Williams, Richard A J; Timm, Robert M; Perez-Tris, Javier; Benitez, Laura

    2015-01-01

    Sylvilagus floridanus Papillomavirus (SfPV) causes growth of large horn-like tumors on rabbits. SfPV was described in cottontail rabbits (probably Sylvilagus floridanus) from Kansas and Iowa by Richard Shope in 1933, and detected in S. audubonii in 2011. It is known almost exclusively from the US Midwest. We explored the University of Kansas Natural History Museum for historical museum specimens infected with SfPV, using molecular techniques, to assess if additional wild species host SfPV, and whether SfPV occurs throughout the host range, or just in the Midwest. Secondary aims were to detect distinct strains, and evidence for strain spatio-temporal specificity. We found 20 of 1395 rabbits in the KU collection SfPV symptomatic. Three of 17 lagomorph species (S. nuttallii, and the two known hosts) were symptomatic, while Brachylagus, Lepus and eight additional Sylvilagus species were not. 13 symptomatic individuals were positive by molecular testing, including the first S. nuttallii detection. Prevalence of symptomatic individuals was significantly higher in Sylvilagus (1.8%) than Lepus. Half of these specimens came from Kansas, though new molecular detections were obtained from Jalisco-Mexico's first-and Nebraska, Nevada, New Mexico, and Texas, USA. We document the oldest lab-confirmed case (Kansas, 1915), pre-dating Shope's first case. SfPV amplification was possible from 63.2% of symptomatic museum specimens. Using multiple methodologies, rolling circle amplification and, multiple isothermal displacement amplification in addition to PCR, greatly improved detection rates. Short sequences were obtained from six individuals for two genes. L1 gene sequences were identical to all previously detected sequences; E7 gene sequences, were more variable, yielding five distinct SfPV1 strains that differing by less than 2% from strains circulating in the Midwest and Mexico, between 1915 and 2005. Our results do not clarify whether strains are host species specific, though

  18. Light colour preference of growing rabbits

    Directory of Open Access Journals (Sweden)

    Zsolt Szendrő

    2010-01-01

    Full Text Available The objective of the experiment was to evaluate the light colour preference of growing rabbits placed in a free-choice cage. The experiment was carried out on 128 Pannon White growing rabbits weaned at the age of 5 weeks and placed into blocks (2m2 of four cages. The rabbits could move freely among the four cages (0.5m2 each through swing doors. The cages differed only in the colour of the light applied (white, yellow, green or blue. The lighting schedule was 16L: 8D. From 6 until 10 weeks of age, infrared video recording was performed once a week (24 hours. The number of rabbits in each cage was counted every 15 minutes. Feed consumption was measured weekly. Between 6 and 10 weeks of age the rabbits significantly preferred white light (28.0%. The preference order was the following: yellow (26.3%, blue (23.4% and green (22.3% (P<0.001. No significant differences were recorded in the feed consumption among the cages. In conclusion, the cage preference of the rabbits was slightly affected by the light colour.

  19. Ionic composition of the earth's radiation belts

    Science.gov (United States)

    Spjeldvik, W. N.

    1983-01-01

    Several different ion species have been positively identified in the earth's radiation belts. Besides protons, there are substantial fluxes of helium, carbon and oxygen ions, and there are measurable quantities of even heavier ions. European, American and Soviet space experimenters have reported ion composition measurements over wide ranges of energies: at tens of keV (ring-current energies) and below, and at hundreds of keV and above. There is still a gap in the energy coverage from several tens to several hundreds of keV where little observational data are available. In this review emphasis is placed on the radiation belt ionic structure above 100 keV. Both quiet time conditions and geomagnetic storm periods are considered, and comparison of the available space observations is made with theoretical analysis of geomagnetically trapped ion spatial, energy and charge state distributions.

  20. HP metamorphic belt of the western Alps

    Institute of Scientific and Technical Information of China (English)

    RobertoCompagnoni

    2003-01-01

    The understanding of the subduction-related processes benefited by the studies of the high-pressure (HP) meta-morphic rocks from the western Alps. The most stimu-lating information was obtained from the inner part of the western Alpine belt, where most tectonic units show an early Alpine eclogite-facies recrystallisation. This is especially true for the Austroalpine Sesia Zone and the Penninic Dora-Maira massif. From the Sesia zone,which consists of a wide spectrum of continental crust lithologies recrystallised to quartz-eclogite-facies min-eral assemblages, the first finding of a jadeite-bearingmeta-granitoid has been described, supporting evidencethat even continental crust may subduct into the mantle.From the Dora-Maira massif the first occurrence of regional metamorphic coesite has been reported, open-ing the new fertile field of the ultrahigh-pressure meta-morphism (UHPM), which is now becoming the rule in the collisional orogenic belts.

  1. Therapeutic activity of crude ethanolic extract of Artemisia herba alba against Trypanosoma evansi in rabbits

    Science.gov (United States)

    Awad, Fathy M.; Hasan, Zainal Abidin Abu; Osman, Abdinasir Yusuf; Ibrahim, Nazlina

    2013-11-01

    The present work was conducted to evaluate the antitrypanosomal efficacy of crude ethanolic extract (CEE) of the aerial parts of Artemisia herba alba against Trypanosoma evansi infection in an animal model. The results indicated low levels of parasitaemia in rabbits administered with crude ethanolic extract (CEE) compared to those from the negative control group. Similarly, there was also haematologically significant difference (p<0.05) where low mean levels of packed cell volume (PCV) was observed in Groups 1-4 respectively. In contrast, there was no statistically significant difference in almost all investigated parameters between positive control and treatment groups of animals. In conclusion, both CEE of A. herba-alba and Berenil® showed relatively a parasitaemia and normal haematological values in infected rabbits, thereby confirming their antiparasitic properties.

  2. Alien Asteroid Belt Compared to our Own

    Science.gov (United States)

    2005-01-01

    [figure removed for brevity, see original site] Figure 1: Band of Light Comparison This artist's concept illustrates what the night sky might look like from a hypothetical alien planet in a star system with an asteroid belt 25 times as massive as the one in our own solar system (alien system above, ours below; see Figure 1). NASA's Spitzer Space Telescope found evidence for such a belt around the nearby star called HD 69830, when its infrared eyes spotted dust, presumably from asteroids banging together. The telescope did not find any evidence for a planet in the system, but astronomers speculate one or more may be present. The movie begins at dusk on the imaginary world, when HD 69830, like our Sun, has begun to set over the horizon. Time is sped up to show the onset of night and the appearance of a brilliant band of light. This light comes from dust in a massive asteroid belt, which scatters sunlight. In our solar system, anybody observing the skies on a moonless night far from city lights can see the sunlight that is scattered by dust in our asteroid belt. Called zodiacal light and sometimes the 'false dawn,' this light appears as a dim band stretching up from the horizon when the Sun is about to rise or set. The light is faint enough that the disk of our Milky Way galaxy remains the most prominent feature in the sky. (The Milky Way disk is shown perpendicular to the zodiacal light in both pictures.) In contrast, the zodiacal light in the HD 69830 system would be 1,000 times brighter than our own, outshining even the Milky Way.

  3. The atmospheric implications of radiation belt remediation

    Directory of Open Access Journals (Sweden)

    C. J. Rodger

    2006-08-01

    Full Text Available High altitude nuclear explosions (HANEs and geomagnetic storms can produce large scale injections of relativistic particles into the inner radiation belts. It is recognised that these large increases in >1 MeV trapped electron fluxes can shorten the operational lifetime of low Earth orbiting satellites, threatening a large, valuable population. Therefore, studies are being undertaken to bring about practical human control of the radiation belts, termed "Radiation Belt Remediation" (RBR. Here we consider the upper atmospheric consequences of an RBR system operating over either 1 or 10 days. The RBR-forced neutral chemistry changes, leading to NOx enhancements and Ox depletions, are significant during the timescale of the precipitation but are generally not long-lasting. The magnitudes, time-scales, and altitudes of these changes are no more significant than those observed during large solar proton events. In contrast, RBR-operation will lead to unusually intense HF blackouts for about the first half of the operation time, producing large scale disruptions to radio communication and navigation systems. While the neutral atmosphere changes are not particularly important, HF disruptions could be an important area for policy makers to consider, particularly for the remediation of natural injections.

  4. Influence of body temperature on bacterial growth rates in experimental pneumococcal meningitis in rabbits.

    OpenAIRE

    Small, P M; Täuber, M G; Hackbarth, C J; Sande, M A

    1986-01-01

    We examined the role of fever as a host defense in experimental pneumococcal meningitis in rabbits. Twelve hours after intracisternal inoculation of an encapsulated type 3 Streptococcus pneumoniae strain, body temperature was manipulated by using two different anesthetic drugs: pentobarbital, which did not affect temperature, and urethane, which mitigated the febrile response to infection. Growth rates of pneumococci in cerebrospinal fluid were dramatically influenced by modification of the f...

  5. Seat belt use during pregnancy in Iran: attitudes and practices

    Institute of Scientific and Technical Information of China (English)

    Mojgan Karbakhsh; Zahra Ershadi; Ali Khaji; Fatemeh Rahimi-Sharbaf

    2010-01-01

    Objective: Seat belt use during pregnancy reduces injury to the mother and her fetus. During recent years, the use of seat belts has been mandated by law in Iran. The purpose of this study was to determine the attitudes and practices of pregnant women regarding seat belt use.Methods: In this cross-sectional study, we asked 335 pregnant women at a hospital-based prenatal care clinic on the use of safety belt before and during pregnancy. SPSS version 13.0 was used for data analysis.Results: The mean age of study subjects was 27.3 years±5.3 years with the median of 27 years. Compared with the seat belt use before pregnancy, no change was detected in 48.7% of the women; seat belt use had increased in 17.5 %of them and decreased in 33.8 %. Eighty-one percent of women knew the correct placing of both lap belt and shoulder belt. Only 4% of women had received education on proper restraint use during pregnancy.Conclusions: The prevalence of seat belt use during pregnancy is lower than reports which are mostly from developed nations. The fact that about one-third of women have decreased their seat belt usage during pregnancy highlights the importance of education of mothers on this topic.

  6. Self-assembly of virus-like particles of rabbit hemorrhagic disease virus capsid protein expressed in Escherichia coli and their immunogenicity in rabbits.

    Science.gov (United States)

    Guo, Huimin; Zhu, Jie; Tan, Yonggui; Li, Chuanfeng; Chen, Zongyan; Sun, Shiqi; Liu, Guangqing

    2016-07-01

    In this study, virus-like particles (VLPs) derived from rabbit hemorrhagic disease virus (RHDV) were evaluated for the development of a vaccine against RHDV infection. The VP60 gene was cloned and inserted into a pSMK expression vector containing a small ubiquitin-like modifier (SUMO) tag that can promote the soluble expression of heterologous proteins in Escherichia coli cells. After expression and purification of His-SUMO-VP60 and cleavage of the SUMO tag, we found that the RHDV VP60 protein had self-assembled into VLPs with a similar shape and smaller size compared with authentic RHDV capsid. Next, the antigenicity and immunogenicity of the VLPs were examined. The results showed that RHDV-specific responses were clearly induced in rabbits and that all rabbits in the VLP group survived while those in the negative control group died within 72 h post-infection. These results suggest that VLP-based RHDV could be a promising RHDV vaccine candidate.

  7. CURRENT STATUS, CHALLENGES AND OPPORTUNITIES OF RABBIT PRODUCTION IN BOTSWANA

    Directory of Open Access Journals (Sweden)

    J.C. MOREKI

    2012-03-01

    Full Text Available This review highlights the current status of rabbit production, challenges facing the industry and opportunities available. Rabbit farming in Botswana is in its infancy and the rabbit population is estimated to be less than 1000. However, this value is a gross underestimate due to poor monitoring by government extension services. In Botswana, rabbits are mainly kept in the backyards, indicating that intensive systems have not yet been developed. Rabbits have small body size, short gestation period, high reproductive potential, rapid growth rate and ability to utilize forages. Compared to beef, chicken, mutton, chevon and chicken, rabbit meat has low cholesterol, high protein and low fat contents. Rabbit production can be integrated into small farming systems, with the rabbits being fed on crop residues, weeds, poultry droppings, and kitchen and garden wastes. The manure can be used to fertilize soils. The major challenges in rabbit production are inadequacy of breeding stock, inadequate rabbit feeds, poor management (feeding, housing and health care, lack of research support, lack of technical support from extension services, lack of access to credit and inadequate supply of equipment. The major opportunity available to the rearers is that the market is vast due to the small rabbit population in the country. The attributes of rabbits suggest that rabbit farming is likely to play an important role in nutrition, poverty alleviation and food security, especially in countries with higher unemployment levels and HIV/AIDS prevalence rates such as Botswana.

  8. Lack of effectiveness of ofloxacin against experimental syphilis in rabbits.

    Science.gov (United States)

    Une, T; Nakajima, R; Otani, T; Katami, K; Osada, Y; Otani, M

    1987-09-01

    Ofloxacin, a new pyridone-carboxylic acid derivative, was evaluated in experimental syphilis in rabbits in comparison with penicillin G. Experimental syphilis was established by intradermal injection of Treponema pallidum subsp. pallidum Nichols. Ten days after infection, the dermal lesions were characterized by syphilitic papula accompanied with central necrosis. These animals were subsequently treated either with ofloxacin twice a day at an oral dose of 10 mg/kg or with penicillin G once a day at an intramuscular dose of 10,000 U/kg for 21 consecutive days. In penicillin G-treated animals, the dermal lesions became smaller as early as day 3 of treatment and almost disappeared during the therapy. In marked contrast to remarkable efficacy of penicillin G was further development of the lesions in ofloxacin-treated animals, showing no difference in pathological manifestations as compared to untreated animals. The results of nontreponemal serologic test correlated well with the response of animals to treatment.

  9. Modal analysis of coupled vibration of belt drive systems

    Institute of Scientific and Technical Information of China (English)

    LI Xiao-jun; CHEN Li-qun

    2008-01-01

    The modal method is applied to analyze coupled vibration of belt drive systems. A belt drive system is a hybrid system consisting of continuous belts modeled as strings as well as discrete pulleys and a tensioner arm. The characteristic equation of the system is derived from the governing equation. Numerical results demenstrate the effects of the transport speed and the initial tension on natural frequencies.

  10. Hypercholesterolemia impaired sperm functionality in rabbits.

    Directory of Open Access Journals (Sweden)

    Tania E Saez Lancellotti

    Full Text Available Hypercholesterolemia represents a high risk factor for frequent diseases and it has also been associated with poor semen quality that may lead to male infertility. The aim of this study was to analyze semen and sperm function in diet-induced hypercholesterolemic rabbits. Twelve adult White New Zealand male rabbits were fed ad libitum a control diet or a diet supplemented with 0.05% cholesterol. Rabbits under cholesterol-enriched diet significantly increased total cholesterol level in the serum. Semen examination revealed a significant reduction in semen volume and sperm motility in hypercholesterolemic rabbits (HCR. Sperm cell morphology was seriously affected, displaying primarily a "folded head"-head fold along the major axe-, and the presence of cytoplasmic droplet on sperm flagellum. Cholesterol was particularly increased in acrosomal region when detected by filipin probe. The rise in cholesterol concentration in sperm cells was determined quantitatively by Gas chromatographic-mass spectrometric analyses. We also found a reduction of protein tyrosine phosphorylation in sperm incubated under capacitating conditions from HCR. Interestingly, the addition of Protein Kinase A pathway activators -dibutyryl-cyclic AMP and iso-butylmethylxanthine- to the medium restored sperm capacitation. Finally, it was also reported a significant decrease in the percentage of reacted sperm in the presence of progesterone. In conclusion, our data showed that diet-induced hypercholesterolemia adversely affects semen quality and sperm motility, capacitation and acrosomal reaction in rabbits; probably due to an increase in cellular cholesterol content that alters membrane related events.

  11. Genotoxic effects of copper sulfate in rabbits

    Directory of Open Access Journals (Sweden)

    Georgieva S.

    2013-01-01

    Full Text Available This study was carried out to determine the genotoxic effects of oral application of CuSO4 in rabbits by the chromosome aberration (CA and sister chromatid exchange (SCE tests. Ten male New Zealand rabbits (5 months old, weighing 3.5-4.0 kg were allocated into two groups. The first group received CuSO4 (5H2O in drinking water for 6 consecutive days. The second group was used as a control. On the 7th day, blood samples were taken from the ear marginal vein and the SCE and CA tests in peripheral lymphocytes were used as genotoxicity and mutagenicity endpoints, respectively. Results showed a significant increase in the frequencies of the aberrant cells (7.4±0.24, P<0.001 and CA (chromatid fragments 3.2±0.37, chromosome fragments 4.2±0.37, P<0.001, and total aberrations (7.4±0.24, P<0.001 after the treatment with CuSO4 when compared with the control group. The level of SCE per cell in the CuSO4-treated rabbits (9.66±0.062 was significantly higher than in rabbits from the control group. These findings show that copper exhibits a genotoxic and mutagenic potential in rabbits.

  12. Review of experimental models: sinusitis in rabbits

    Directory of Open Access Journals (Sweden)

    André Coura Perez

    2014-10-01

    Full Text Available INTRODUCTION: In order to better understand the pathophysiology of rhinosinusitis, several attempts have been made to create the disease in an animal model. Among the studied rodents each has its advantages and disadvantages. Rabbits are considered more appropriate for studies that require surgical manipulation or invasive procedures. OBJECTIVES: To evaluate the most viable experimental model of rhinosinusitis in rabbits to be adopted in future studies. METHODS: An electronic search for studies with experimental models of rhinosinusitis in rabbits published in English and Portuguese between July of 1967 and January of 2013 was conducted in Medline, Pub Med, Cochrane, and CAPES databases, using the keywords "sinusitis", "rabbits", and "polyps". RESULTS: A total of 256 studies were retrieved, but in accordance with the inclusion and exclusion criteria, only ten studies were selected. Many different methods of response assessment were used in these studies. CONCLUSION: To date, there is no ideal experimental model for induction of acute or chronic rhinosinusitis in rabbits, but the rhinogenic model appears to be the most viable option for the continuity of studies of the disease.

  13. Hypercholesterolemia Impaired Sperm Functionality in Rabbits

    Science.gov (United States)

    Monclus, Maria A.; Cabrillana, Maria E.; Clementi, Marisa A.; Espínola, Leandro S.; Cid Barría, Jose L.; Vincenti, Amanda E.; Santi, Analia G.; Fornés, Miguel W.

    2010-01-01

    Hypercholesterolemia represents a high risk factor for frequent diseases and it has also been associated with poor semen quality that may lead to male infertility. The aim of this study was to analyze semen and sperm function in diet-induced hypercholesterolemic rabbits. Twelve adult White New Zealand male rabbits were fed ad libitum a control diet or a diet supplemented with 0.05% cholesterol. Rabbits under cholesterol-enriched diet significantly increased total cholesterol level in the serum. Semen examination revealed a significant reduction in semen volume and sperm motility in hypercholesterolemic rabbits (HCR). Sperm cell morphology was seriously affected, displaying primarily a “folded head”-head fold along the major axe-, and the presence of cytoplasmic droplet on sperm flagellum. Cholesterol was particularly increased in acrosomal region when detected by filipin probe. The rise in cholesterol concentration in sperm cells was determined quantitatively by Gas chromatographic-mass spectrometric analyses. We also found a reduction of protein tyrosine phosphorylation in sperm incubated under capacitating conditions from HCR. Interestingly, the addition of Protein Kinase A pathway activators -dibutyryl-cyclic AMP and iso-butylmethylxanthine- to the medium restored sperm capacitation. Finally, it was also reported a significant decrease in the percentage of reacted sperm in the presence of progesterone. In conclusion, our data showed that diet-induced hypercholesterolemia adversely affects semen quality and sperm motility, capacitation and acrosomal reaction in rabbits; probably due to an increase in cellular cholesterol content that alters membrane related events. PMID:20976152

  14. Identification of genes transcribed by Pasteurella multocida in rabbit livers through the selective capture of transcribed sequences.

    Science.gov (United States)

    Guo, Dongchun; Lu, Yan; Zhang, Aiqin; Liu, Jiasen; Yuan, Dongwei; Jiang, Qian; Lin, Huan; Si, Changde; Qu, Liandong

    2012-06-01

    Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera in poultry, hemorrhagic septicemia in cattle, atropic rhinitis in swine, and snuffles in rabbits. The differentially expressed gene profile of P. multocida in infected rabbit livers was identified and compared with that from in vitro culture by selective capture of transcribed sequences. A total of 31 genes were identified, of which 28 encoded enzymes for amino acid biosynthesis and metabolism, intermediary metabolism, and energy metabolism, or proteins for regulatory adaptive responses, general microbial stress response, transport proteins, and secreted proteinases. Three were unknown, novel genes. Five genes representing different categories were chosen randomly and verified by real-time reverse transcriptase-polymerase chain reaction analysis. All were upregulated by P. multocida in infected rabbit livers, with changes ranging from 1.61- to 13.55-fold when compared with in vitro cultures. This study has identified genes of P. multocida that are upregulated during infection of rabbit livers when compared with in vitro growth conditions. The genes will provide a molecular basis for further study of the pathogenesis of P. multocida.

  15. Diagnosis of dental problems in pet rabbits (Oryctolagus cuniculus)

    OpenAIRE

    2008-01-01

    Dental problems are very common in pet rabbits. To establish a correct diagnosis of rabbit dental pathology, a general knowledge of normal dental anatomy and physiology is necessary. The specific anatomy and the most common pathologies of rabbit dentition are reviewed. Techniques for diagnosing dental abnormalities - such as clinical examination, radiography and computed tomography (CT) - are summarized. Finally two clinical cases of rabbits with dental pathologies are described.

  16. Immunodiagnosis of systemic aspergillosis. I. Antigenemia detected by radioimmunoassay in experimental infection. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Weiner, M.H.; Coats-Stephen, M.

    1979-01-01

    Because systemic aspergillosis is difficult to diagnose ante mortem, a study to improve immunodiagnosis was undertaken in a rabbit model of disseminated infection. We found that the predominant humoral response of infected animals was directed against four Aspergillus antigens identified by crossed immunoelectrophoresis. One of these antigens, a cell-wall carbohydrate, was purified by gel-filtration chromatography and was used to develop a radiommunoassay. The sensitivity of this assay was increased by testing for serum-bound antigen as well as for free antigen. When the sensitivity of the RIA was evaluated in the animal model, antigenemia was detected in 78% of 51 rabbits with disseminated infection and ante mortem in 86% of 42 rabbits with lethal infection. By contrast, with immunoprecipitin analysis only eight of 51 rabbits were positive for antigen, and six of 51 rabbits were positive for Aspergillus antibody. The specificity of the RIA was also tested. Negative controls for antigen included sera from 76 normal rabbits and sera from 25 rabbits with systemic candidiasis. The Candida control group is pertinent because 48% of these rabbits had specific Candida antigenemia detected by a mannan RIA. This study demonstrates that Aspergillus antigenemia occurs during the course of experimental disseminated aspergillosis and illustrates the potential of an Aspergillus antigen RIA for sensitive, specific immunodiagnosis of human infections.

  17. The Radiation Belt Storm Probes Mission: Advancing Our Understanding of the Earth's Radiation Belts

    Science.gov (United States)

    Sibeck, David; Kanekal, Shrikanth; Kessel, Ramona; Fox, Nicola; Mauk, Barry

    2012-01-01

    We describe NASA's Radiation Belt Storm Probe (RBSP) mission, whose primary science objective is to understand, ideally to the point of predictability, the dynamics of relativistic electrons and penetrating ions in the Earth's radiation belts resulting from variable solar activity. The overarching scientific questions addressed include: 1. the physical processes that produce radiation belt enhancement events, 2. the dominant mechanisms for relativistic electron loss, and 3. how the ring current and other geomagnetic processes affect radiation belt behavior. The RBSP mission comprises two spacecraft which will be launched during Fall 2012 into low inclination lapping equatorial orbits. The orbit periods are about 9 hours, with perigee altitudes and apogee radial distances of 600 km and 5.8 RE respectively. During the two-year primary mission, the spacecraft orbits precess once around the Earth and lap each other twice in each local time quadrant. The spacecraft are each equipped with identical comprehensive instrumentation packages to measure, electrons, ions and wave electric and magnetic fields. We provide an overview of the RBSP mission, onboard instrumentation and science prospects and invite scientific collaboration.

  18. Effects of Rosiglitazone on Rabbit Atherosclerosis

    Institute of Scientific and Technical Information of China (English)

    ZHAO Qing; WEI Meng; ZHAO Bing-hui

    2008-01-01

    We sought to validate magnetic resonance imaging (MRI) for documenting the effects of rosiglitazone on rabbit atherosclerosis and the possible mechanism by treatment on the lesions.24 New Zealand white rabbits were randomly divided into normal group,control group and treatment group.After 4 weeks,all rabbits underwent MRI.After examination,the number of circulating endothelial progenitor cells (EPCs) and level of lipid,glucose,nitric oxide (NO) and superoxide anion (O-2.) were measured.MRI demonstrated the regression of atherosclerotic lesions by rosiglitazone which has good correlation with histopathology.Treatment not only increased the level of EPCs and NO,but also raised the ability of inhibition of O-2..MRI is a promising noninvasive technology to detect the effects of therapeutic interventions.Rosiglitazone slows the progression of atherosclerosis that may in part attribute to its improvement of mobilization of EPCs and the reduced oxidative stress.

  19. CHAOTIC BELT PHENOMENA IN NONLINEAR ELASTIC BEAM

    Institute of Scientific and Technical Information of China (English)

    张年梅; 杨桂通

    2003-01-01

    The chaotic motions of axial compressed nonlinear elastic beam subjected totransverse load were studied. The damping force in the system is nonlinear. Consideringmaterial and geometric nonlinearity, nonlinear governing equation of the system wasderived. By use of nonlinear Galerkin method, differential dynamic system was set up.Melnikov method was used to analyze the characters of the system. The results showed thatchaos may occur in the system when the load parameters P0 and f satisfy some conditions.The zone of chaotic motion was belted. The route from subharmonic bifurcation to chaoswas analyzed. The critical conditions that chaos occurs were determined.

  20. Parametric resonances of convection belt system

    Institute of Scientific and Technical Information of China (English)

    Zhi-an YANG; Gao-feng LI

    2009-01-01

    Based on the Coriolis acceleration and the Lagrangian strain formula,a generalized equation for the transverse vibration system of convection belts is derived using Newton's second law.The method of multiple scales is directly applied to the governing equations,and an approximate solution of the primary parameter resonance of the system is obtained.The detuning parameter,cross-section area,elastic and viscoelastic parameters,and axial moving speed have a significant influences on the amplitudes of steady-state response and their existence boundaries.Some new dynamical phenomena are revealed.

  1. Orion revisited. III. The Orion Belt population

    Science.gov (United States)

    Kubiak, K.; Alves, J.; Bouy, H.; Sarro, L. M.; Ascenso, J.; Burkert, A.; Forbrich, J.; Großschedl, J.; Hacar, A.; Hasenberger, B.; Lombardi, M.; Meingast, S.; Köhler, R.; Teixeira, P. S.

    2017-02-01

    Aims: This paper continues our study of the foreground population to the Orion molecular clouds. The goal is to characterize the foreground population north of NGC 1981 and to investigate the star formation history in the large Orion star-forming region. We focus on a region covering about 25 square degrees, centered on the ɛ Orionis supergiant (HD 37128, B0 Ia) and covering the Orion Belt asterism. Methods: We used a combination of optical (SDSS) and near-infrared (2MASS) data, informed by X-ray (XMM-Newton) and mid-infrared (WISE) data, to construct a suite of color-color and color-magnitude diagrams for all available sources. We then applied a new statistical multiband technique to isolate a previously unknown stellar population in this region. Results: We identify a rich and well-defined stellar population in the surveyed region that has about 2000 objects that are mostly M stars. We infer the age for this new population to be at least 5 Myr and likely 10 Myr and estimate a total of about 2500 members, assuming a normal IMF. This new population, which we call the Orion Belt population, is essentially extinction-free, disk-free, and its spatial distribution is roughly centered near ɛ Ori, although substructure is clearly present. Conclusions: The Orion Belt population is likely the low-mass counterpart to the Ori OB Ib subgroup. Although our results do not rule out Blaauw's sequential star formation scenario for Orion, we argue that the recently proposed blue streams scenario provides a better framework on which one can explain the Orion star formation region as a whole. We speculate that the Orion Belt population could represent the evolved counterpart of an Orion nebula-like cluster. The catalog (Full Table A.1) is only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/598/A124

  2. Period Determination of Six Main Belt Asteroids

    Science.gov (United States)

    Ferrero, Andrea

    2014-07-01

    Observations of six main-belt asteroids (MBA) produced lightcurve parameters of: 487 Venetia, P = 13.34 ± 0.01 h, A = 0.20 mag; 684 Hildburg, P = 15.89 ± 0.01 h, A = 0.22 mag; 772 Tanete, P = 8.629 ± 0.001 h, A = 0.18 mag.; 1181 Lilith, P = 15.04 ± 0.01 h, A = 0.11 mag.; 1246 Chaka, P = 25.44 ± 0.01 h, A = 0.25 mag.; and 2834 Christy Carol, P = 12.79 ± 0.01 h, A = 0.39 mag.

  3. Water activities in the Kerala Khondalite Belt

    Science.gov (United States)

    Chacko, Thomas; Kumar, G. R. Ravindra; Peterson, J. W.

    1988-01-01

    The author and colleagues presented their determinations of water activities in various granulite-facies rocks of the Kerala Khondalite Belt. Using mineral equilibria, thermodynamic data, and assumed geopressure-geotemperature conditions of 5.5 kbar and 750 C, they calculated uniformly low a(H2O) values of about 0.27 over a large geographic region. They suggested that these conditions were produced by the presence of abundant CO2-rich fluids, derived either from deeper levels or from metamorphic reactions involving graphite.

  4. Solar Neutrons and the Earth's Radiation Belts.

    Science.gov (United States)

    Lingenfelter, R E; Flamm, E J

    1964-04-17

    The intensity and spectrum of solar neutrons in the vicinity of the earth are calculated on the assumption that the low-energy protons recently detected in balloon and satellite flights are products of solar neutron decay. The solar-neutron flux thus obtained exceeds the global average cosmic-ray neutron leakage above 10 Mev, indicating that it may be an important source of both the inner and outer radiation belts. Neutron measurements in the atmosphere are reviewed and several features of the data are found to be consistent with the estimated solar neutron spectrum.

  5. Linear Motor for Drive of Belt Conveyor

    Directory of Open Access Journals (Sweden)

    Milan Krasl

    2006-01-01

    Full Text Available This paper introduces a novel approach on the design of a linear motor for drive of belt conveyor (LMBC. The motor is a simple combination of asynchronous motor in plane. The electromagnetic forces is one of the most important parameters of electrical machines. This parameter is necessary for the checking of the design. This paper describes several variants: linear motor with slots in platens, slots in one half of platens and optimization of slots. The electromagnetic force can be found with the help of a Finite Elements Method – based program. For solution was used QuickField program.

  6. Hydrogel iontophoresis for gentamicin administration to the rabbit eye

    Science.gov (United States)

    Eljarrat-Binstock, Esther; Raiskup, Frederik; Frucht-Pery, Joseph; Domb, Abraham J.

    2005-04-01

    Iontophoresis (IONT) is a non-invasive technique in which a low electric current is used to enhance the penetration of charged molecules into tissue. This technique has been used in various fields of medicine, mostly in transdermal drug delivery. This study was aimed to evaluate the efficacy and the distribution profile of gentamicin using corneal IONT on infected and healthy rabbit eyes. Corneal iontophoresis of gentamicin sulfate was studied using drug-loaded disposable hydrogel probes mounted on a portable iontophoretic device, applying a low current for 60 seconds. This study confirmed that a triple iontophoretic treatment of gentamicin for only 60 seconds (0.5mA) significantly reduces the count of pseudomonas in the infected cornea to a non-infectious level. Peak gentamicin concentrations at the healthy corneas (363.1 +/- 127.3 μg/g) and at the aqueous humor (29.4 +/- 17.4 μg/ml) were reached immediately and two hours after a single iontophoretic treatment, respectively. The concentration versus time profile of gentamicin following iontophoresis revealed a gentamicin half life of 2.07 h in the anterior chamber, and a clearance of 1.73 μl/min from the anterior chamber to the posterior segments of the eye. This study indicates that a short iontophoretic treatment using gentamicin-loaded hydrogels has a potential clinical value in treating corneal infections, by increasing drug penetration to the eye and maintaining therapeutic levels for more than eight hours.

  7. A Preliminary internet survey of pet rabbit owners’ characteristics

    Directory of Open Access Journals (Sweden)

    J.A. Oxley

    2015-12-01

    Full Text Available This study aimed to conduct a preliminary survey to investigate basic ownership factors, frequency of microchipping and insurance and views of pet rabbit owners  n these areas and general rabbit management. More specifically, we aimed to investigate whether owners possess insurance, whether their rabbits are microchipped, and owners’ views on the recommendations relating to rabbits (e.g. recommended enclosure sizes and the law. A questionnaire was designed and promulgated through social media sites and rabbit forums. A total of 1183 responses were received. Just over 29% of respondents sourced their rabbits through rescue centres. 73.9% (867/1174 of owners stated that they had no pet insurance for their rabbits. Concerning microchips, 78.3% (919/1173 of rabbits were not microchipped, while 21.7% (254/1173 were. This preliminary study found that the majority of individuals are of the opinion that the relevant law is insufficiently publicised. A more detailed study would be beneficial to investigate and provide further insight into rabbit owners and their views and concerns for rabbits. The results of such a study could help formulate rabbit-related information and guidelines which in turn could have a direct impact on pet rabbit welfare.

  8. Effect of trichlorfon on selenium distribution in rabbits.

    Science.gov (United States)

    Kossakowski, S; Dziura, A

    1987-01-01

    The investigations were performed on 94 rabbits intoxicated with trichlorfon which were untreated or treated. The intoxicated rabbits were given per os Na75SeO3. Selenium distribution in different organs and tissues was examined radiometrically. In intoxicated untreated rabbits and in those at the beginning of treatment decreased selenium level was found.

  9. Images in Rabbit, Run from Archetypal Perspective%Images in Rabbit,Run from Archetypal Perspective

    Institute of Scientific and Technical Information of China (English)

    付晓芳

    2016-01-01

    John Updike is regarded as one of the greatest American novelists in the 20th century. His Rabbit series brought him a worldwide reputation, especially the first one Rabbit, Run is considered as his magnum opus and has drawn the most concern from the critics. This thesis is intended to shed light on Rabbit, Run by applying some important archetypal terms, especially the archetypal images. By sorting out the employment of images, this thesis aims to prove Harry's plight and to dig out the theme of the empty mental condition of the whole society. Therefore, it hopes to provide a new way to interpret this novel.

  10. Teratogenic effect of formaldehyde in rabbits

    Directory of Open Access Journals (Sweden)

    A. A. Al–Saraj

    2009-01-01

    Full Text Available Thirty three pregnant rabbits were exposed to vapour of 10% formaldehyde (12 ppm throughout the gestation period to know its effect on newborns. The results showed no abortion or foetal mortality but there were some anomalies (23.8% among the newborns rabbits which includes: meromelia (6.8%, encephalocele (6.1%, Oligodactyly (4.1%, Umbilical hernia (3.4% and Short tail (3.4%; besides that small for date and decrease in the body weight of the newborns were also noticed. These findings suggest that formaldehyde is a teratogenic agent.

  11. Detection of pathological lesions in slaughtered rabbits

    Directory of Open Access Journals (Sweden)

    Guido Grilli

    2010-01-01

    Full Text Available The slaughterhouse is considered an important control point for the monitoring of rabbit diseases. In our study, 59,440 rabbit carcasses were examined, but only 1% of pathological lesions were recorded at postmortem inspection. Mainly affected were tegumentary, digestive and urinary systems. The most consistent lesion was the subcutaneous abscess; nephritis, probably caused by Encephalitozoon cuniculi, was also frequent. Pathological alterations of the liver, classified as “necrotizing hepatitis” and localized at the caudate lobe, were observed for the first time.

  12. Experimental Yersinia enterocolitica enteritis in rabbits.

    OpenAIRE

    Pai, C H; Mors, V; Seemayer, T. A.

    1980-01-01

    Young rabbits weighing 500 to 800 g were inoculated orogastrically with clinical isolates of Yersinia enterocolitica (serotype O:3; enterotoxigenic; HeLa cell invasive) at a dose of 1.4 X 10(10) bacteria suspended in 10% sodium bicarbonate solution. Diarrhea developed in 41 (87%) of 47 rabbits, with a mean +/- standard deviation onset at 5.4 +/- 2.4 days. The attack rate and onset of diarrhea were correlated with inoculum size. The 50% infectious dose was 2.9 X 10(8) bacteria. Bacterial colon...

  13. Plasma PIVKA proteins in rabbits given warfarin.

    Science.gov (United States)

    Zivelin, A; Rao, L V; Rapaport, S I

    1996-06-01

    The presence of partially carboxylated forms of the vitamin K dependent coagulation factors (PIVKA) was evaluated in the plasma of rabbits treated with warfarin. Excess antigen over activity as measured in rabbit specific assays was taken as evidence for PIVKA. Our data confirm a previous report of the absence of plasma PIVKA prothrombin. In contrast, plasma PIVKA factors VII, IX, and X were demonstrable. A striking excess of plasma factor IX antigen over activity was measured and a large fraction of the factor IX antigen persisted in the plasma after its adsorption with barium citrate.

  14. Effects of driver characteristics on seat belt fit.

    Science.gov (United States)

    Reed, Matthew P; Ebert, Sheila M; Hallman, Jason J

    2013-11-01

    A laboratory study of posture and belt fit was conducted with 46 men and 51 women, 61% of whom were age 60 years or older and 32% age 70 years or older. In addition, 28% of the 97 participants were obese, defined as body mass index ≥ 30 kg/m^2. A mockup of a passenger vehicle driver's station was created and five belt anchorage configurations were produced by moving the buckle, outboard-upper (D-ring), and outboard-lower anchorages. An investigator recorded the three-dimensional locations of landmarks on the belt and the participant's body using a coordinate measurement machine. The location of the belt with respect to the underlying skeletal structures was analyzed, along with the length of belt webbing. Using linear regression models, an increase in age from 20 to 80 years resulted in the lap belt positioned 18 mm further forward relative to the pelvis, 26 mm greater lap belt webbing length, and 19 mm greater shoulder belt length. An increase in stature of 350 mm (approximately the range from 5th-percentile female to 95th-percentile male in the U.S. population) was associated with the lap belt 14 mm further forward relative to the pelvis, the shoulder belt 37 mm more outboard relative to the body centerline, and 38 mm less shoulder belt webbing length. Among the driver factors considered, body mass index had the greatest effects. An increase of BMI in 20 kg/m^2, which spans approximately the central 90% of U.S. adults, was associated with the lap belt being placed 102 mm further forward and 94 mm higher, relative to the pelvis, and increases in lap and shoulder belt webbing length of 276 and 258 mm, respectively. Gender did not have important effects on the analyzed belt fit measures after taking into account stature and body mass index. These results offer important considerations for future crash safety assessments and suggest that further research is needed to consider belt fit for older and obese occupants.

  15. Investigation and Analysis of Rabbit Diseases in Guiyang Areas%贵阳市养兔场主要疾病调查分析

    Institute of Scientific and Technical Information of China (English)

    王凡; 金志强; 肖超能; 李谦; 文明

    2012-01-01

    To grasp the epidemic situation of rabbit diseases in rabbit farms in Guiyang areas, the occurrence data of the rabbit diseases, which collected in the pass 10 years in Guiyang areas, was statistically analyzed by search of literature, access of inspection reports and investigation of the scene sites in this study. The results showed that the diarrhea by stimulation of the changed feed and the bacterial infections were two kinds of the major rabbit diseases in the immature rabbits, and infection of Escherichia coli disease, rabbit viral hemorrhagic disease and coccidiosis were the main diseases in the young and adult rabbits. These results provide some reference to prevention and control of diseases in rabbit farms.%为了解贵阳市养兔场疾病发生流行情况,采用文献检索、资料查阅和现场调查等方法对近10年来贵阳市养兔场疾病发生情况进行了统计分析。结果表明:在幼兔阶段主要发生更换饲料应激性腹泻和细菌性疾病,青年兔和成年兔主要发生大肠杆菌病、兔病毒性出血症和兔球虫病。调查结果为贵阳市养兔场疾病的预防与控制提供了参考依据。

  16. Cloning, Characteristics and Functional Analysis of Rabbit NADPH Oxidase 5

    Directory of Open Access Journals (Sweden)

    Feng Chen

    2016-07-01

    Full Text Available Background: Nox5 was the last member of the Nox enzyme family to be identified. Functionally distinct from the other Nox isoforms, our understanding of its physiological significance has been hampered by the absence of Nox5 in mouse and rat genomes. Nox5 is present in the genomes of other species such as the rabbit that have broad utility as models of cardiovascular disease. However, the mRNA sequence, characteristics and functional analysis of rabbit Nox5 has not been fully defined and were the goals of the current study. Methods: Rabbit Nox5 was amplified from rabbit tissue, cloned and sequenced. COS-7 cells were employed for expression and functional analysis via Western blotting and measurements of superoxide. We designed and synthesized miRNAs selectively targeting rabbit Nox5. The nucleotide and amino acid sequences of rabbit Nox5 were aligned with those of putative rabbit isoforms (X1, X2, X3, and X4. A phylogenetic tree was generated based on the mRNA sequence for Nox5 from rabbit and other species. Results: Sequence alignment revealed that the identified rabbit Nox5 was highly conserved with the predicted sequence of rabbit Nox5. Cell based experiments reveal that rabbit Nox5 was robustly expressed and produced superoxide at rest and in a calcium and PMA-dependent manner that was susceptible to superoxide dismutase and the flavoprotein inhibitor, DPI. miRNA-1 was shown to be most effective in down-regulating the expression of rabbit Nox5. Phylogenetic analysis revealed a close relationship between rabbit and armadillo Nox5. Rabbit Nox5 was relatively closely related to human Nox5, but lies in a distinct cluster. Conclusion: Our study establishes the suitability of the rabbit as a model organism to further our understanding of the role of Nox5 in cardiovascular and other diseases and provides new information on the genetic relationship of Nox5 genes in different species.

  17. Single dose adenovirus vectored vaccine induces a potent and long-lasting immune response against rabbit hemorrhagic disease virus after parenteral or mucosal administration.

    Science.gov (United States)

    Fernández, Erlinda; Toledo, Jorge R; Chiong, Maylin; Parra, Francisco; Rodríguez, Elsa; Montero, Carlos; Méndez, Lídice; Capucci, Lorenzo; Farnós, Omar

    2011-08-15

    Rabbit hemorrhagic disease virus (RHDV) is the etiological agent of a lethal and contagious disease of rabbits that remains as a serious problem worldwide. As this virus does not replicate in cell culture systems, the capsid protein gene has been expressed in heterologous hosts or inserted in replication-competent viruses in order to obtain non-conventional RHDV vaccines. However, due to technological or safety issues, current RHDV vaccines are still prepared from organs of infected rabbits. In this work, two human type 5 derived replication-defective adenoviruses encoding the rabbit hemorrhagic disease virus VP60 capsid protein were constructed. The recombinant protein was expressed as a multimer in mouse and rabbit cell lines at levels that ranged from approximately 120 to 160 mg/L of culture. Mice intravenously or subcutaneously inoculated with a single 10(8) gene transfer units (GTU) dose of the AdVP60 vector (designed for VP60 intracellular expression) seroconverted at days 7 and 14 post-immunization, respectively. This vector generated a stronger response than that obtained with a second vector (AdVP60sec) designed for VP60 secretion. Rabbits were then immunized by parenteral or mucosal routes with a single 10(9)GTU dose of the AdVP60 and the antibody response was evaluated using a competition ELISA specific for RHDV or RHDVa. Protective hemagglutination inhibition (HI) titers were also promptly detected and IgG antibodies corresponding with inhibition percentages over 85% persisted up to one year in all rabbits, independently of the immunization route employed. These levels were similar to those elicited with inactivated RHDV or with VP60 obtained from yeast or insect cells. IgA specific antibodies were only found in saliva of rabbits immunized by intranasal instillation. The feasibility of VP60 production and vaccination of rabbits with replication-defective adenoviral vectors was demonstrated.

  18. Inhalational anthrax (Ames aerosol in naive and vaccinated New Zealand rabbits: characterizing the spread of bacteria from lung deposition to bacteremia.

    Directory of Open Access Journals (Sweden)

    Bradford eGutting

    2012-06-01

    Full Text Available There is a need to better understand inhalational anthrax in relevant animal models. This understanding could aid risk assessment, help define therapeutic windows, and provide a better understanding of disease. The aim here was to characterize and quantify bacterial deposition and dissemination in rabbits following exposure to single high aerosol dose (>100LD50 of Bacillus anthracis (Ames spores immediately following exposure through 36 hours. The primary goal of collecting the data was to support investigators in developing computational models of inhalational anthrax disease. Rabbits were vaccinated prior to exposure with the human vaccine (Anthrax Vaccine Adsorbed, AVA or were sham-vaccinated, and were then exposed in pairs (1 sham and 1 AVA so disease kinetics could be characterized in equally-dosed hosts where one group is fully protected and is able to clear the infection (AVA-vaccinated, while the other is susceptible to disease, in which case the bacteria are able to escape containment and replicate uncontrolled (sham-vaccinated rabbits. Between 4-5% of the presented aerosol dose was retained in the lung of sham- and AVA-vaccinated rabbits as measured by dilution plate analysis of homogenized lung tissue or bronchoalveolar lavage (BAL fluid. After 6 and 36 hours, >80% and >96%, respectively, of the deposited spores were no longer detected in BAL, with no detectable difference between sham- or AVA-vaccinated rabbits. Thereafter, differences between the two groups became noticeable. In sham-vaccinated rabbits the bacteria were detected in the tracheobronchial lymph nodes (TBLN 12 hours post exposure and in the circulation at 24 hours, a time point which was also associated with dramatic increases in vegetative CFU in the lung tissue of some animals. In all sham-vaccinated rabbits, bacteria increased in both TBLN and blood through 36 hours at which point in time some rabbits succumbed to disease. In contrast, AVA-vaccinated rabbits showed

  19. Fading of Jupiter's South Equatorial Belt

    Science.gov (United States)

    Sola, Michael A.; Orton, Glenn; Baines, Kevin; Yanamandra-Fisher, Padma

    2011-01-01

    One of Jupiter's most dominant features, the South Equatorial Belt, has historically gone through a "fading" cycle. The usual dark, brownish clouds turn white, and after a period of time, the region returns to its normal color. Understanding this phenomenon, the latest occurring in 2010, will increase our knowledge of planetary atmospheres. Using the near infrared camera, NSFCAM2, at NASA's Infrared Telescope Facility in Hawaii, images were taken of Jupiter accompanied by data describing the circumstances of each observation. These images are then processed and reduced through an IDL program. By scanning the central meridian of the planet, graphs were produced plotting the average values across the central meridian, which are used to find variations in the region of interest. Calculations using Albert4, a FORTRAN program that calculates the upwelling reflected sunlight from a designated cloud model, can be used to determine the effects of a model atmosphere due to various absorption, scattering, and emission processes. Spectra that were produced show ammonia bands in the South Equatorial Belt. So far, we can deduce from this information that an upwelling of ammonia particles caused a cloud layer to cover up the region. Further investigations using Albert4 and other models will help us to constrain better the chemical make up of the cloud and its location in the atmosphere.

  20. Distribution of dust from Kuiper belt objects

    CERN Document Server

    Gorkavyi, N N; Taidakova, T; Mather, J C; Gorkavyi, Nick N.; Ozernoy, Leonid M.; Taidakova, Tanya; Mather, John C.

    2000-01-01

    (Abridged) Using an efficient computational approach, we have reconstructed the structure of the dust cloud in the Solar system between 0.5 and 100 AU produced by the Kuiper belt objects. Our simulations offer a 3-D physical model of the `kuiperoidal' dust cloud based on the distribution of 280 dust particle trajectories produced by 100 known Kuiper belt objects ; the resulting 3-D grid consists of $1.9\\times 10^6$ cells containing $1.2\\times 10^{11}$ particle positions. The following processes that influence the dust particle dynamics are taken into account: 1) gravitational scattering on the eight planets (neglecting Pluto); 2) planetary resonances; 3) radiation pressure; and 4) the Poynting-Robertson (P-R) and solar wind drags. We find the dust distribution highly non-uniform: there is a minimum in the kuiperoidal dust between Mars and Jupiter, after which both the column and number densities of kuiperoidal dust sharply increase with heliocentric distance between 5 and 10 AU, and then form a plateau betwee...

  1. The Dynamical Evolution of the Asteroid Belt

    CERN Document Server

    Morbidelli, Alessandro; O'Brien, David P; Minton, David A; Bottke, William F

    2015-01-01

    The asteroid belt is the leftover of the original planetesimal population in the inner solar system. However, currently the asteroids have orbits with all possible values of eccentricities and inclinations compatible with long-term dynamical stability, whereas the initial planetesimal orbits should have been quasi-circular and almost co-planar. The total mass in the asteroid population is a small fraction of that existing primordially. Also, asteroids with different chemical/mineralogical properties are not ranked in an orderly manner with mean heliocentric distance as one could expect from the existence of a radial gradient of the temperature in the proto-planetary disk, but they are partially mixed. These properties show that the asteroid belt has been severely sculpted by one or a series of processes during its lifetime. This paper reviews the processes that have been proposed so far, discussing the properties that they explain and the problems that they are confronted with. Emphasis is paid to the interpl...

  2. Young Stellar Objects in the Gould Belt

    CERN Document Server

    Dunham, Michael M; Evans, Neal J; Broekhoven-Fiene, Hannah; Cieza, Lucas; Di Francesco, James; Gutermuth, Robert A; Harvey, Paul M; Hatchell, Jennifer; Heiderman, Amanda; Huard, Tracy; Johnstone, Doug; Kirk, Jason M; Matthews, Brenda C; Miller, Jennifer F; Peterson, Dawn E; Young, Kaisa E

    2015-01-01

    We present the full catalog of Young Stellar Objects (YSOs) identified in the 18 molecular clouds surveyed by the Spitzer Space Telescope "cores to disks" (c2d) and "Gould Belt" (GB) Legacy surveys. Using standard techniques developed by the c2d project, we identify 3239 candidate YSOs in the 18 clouds, 2966 of which survive visual inspection and form our final catalog of YSOs in the Gould Belt. We compile extinction corrected SEDs for all 2966 YSOs and calculate and tabulate the infrared spectral index, bolometric luminosity, and bolometric temperature for each object. We find that 326 (11%), 210 (7%), 1248 (42%), and 1182 (40%) are classified as Class 0+I, Flat-spectrum, Class II, and Class III, respectively, and show that the Class III sample suffers from an overall contamination rate by background AGB stars between 25% and 90%. Adopting standard assumptions, we derive durations of 0.40-0.78 Myr for Class 0+I YSOs and 0.26-0.50 Myr for Flat-spectrum YSOs, where the ranges encompass uncertainties in the ado...

  3. Prior Inoculation with Type B Strains of Francisella tularensis Provides Partial Protection against Virulent Type A Strains in Cottontail Rabbits.

    Directory of Open Access Journals (Sweden)

    Vienna R Brown

    Full Text Available Francisella tularensis is a highly virulent bacterium that is capable of causing severe disease (tularemia in a wide range of species. This organism is characterized into two distinct subspecies: tularensis (type A and holarctica (type B which vary in several crucial ways, with some type A strains having been found to be considerably more virulent in humans and laboratory animals. Cottontail rabbits have been widely implicated as a reservoir species for this subspecies; however, experimental inoculation in our laboratory revealed type A organisms to be highly virulent, resulting in 100% mortality following challenge with 50-100 organisms. Inoculation of cottontail rabbits with the same number of organisms from type B strains of bacteria was found to be rarely lethal and to result in a robust humoral immune response. The objective of this study was to characterize the protection afforded by a prior challenge with type B strains against a later inoculation with a type A strain in North American cottontail rabbits (Sylvilagus spp. Previous infection with a type B strain of organism was found to lengthen survival time and in some cases prevent death following inoculation with a type A2 strain of F. tularensis. In contrast, inoculation of a type A1b strain was uniformly lethal in cottontail rabbits irrespective of a prior type B inoculation. These findings provide important insight about the role cottontail rabbits may play in environmental maintenance and transmission of this organism.

  4. Prior Inoculation with Type B Strains of Francisella tularensis Provides Partial Protection against Virulent Type A Strains in Cottontail Rabbits.

    Science.gov (United States)

    Brown, Vienna R; Adney, Danielle R; Olea-Popelka, Francisco; Bowen, Richard A

    2015-01-01

    Francisella tularensis is a highly virulent bacterium that is capable of causing severe disease (tularemia) in a wide range of species. This organism is characterized into two distinct subspecies: tularensis (type A) and holarctica (type B) which vary in several crucial ways, with some type A strains having been found to be considerably more virulent in humans and laboratory animals. Cottontail rabbits have been widely implicated as a reservoir species for this subspecies; however, experimental inoculation in our laboratory revealed type A organisms to be highly virulent, resulting in 100% mortality following challenge with 50-100 organisms. Inoculation of cottontail rabbits with the same number of organisms from type B strains of bacteria was found to be rarely lethal and to result in a robust humoral immune response. The objective of this study was to characterize the protection afforded by a prior challenge with type B strains against a later inoculation with a type A strain in North American cottontail rabbits (Sylvilagus spp). Previous infection with a type B strain of organism was found to lengthen survival time and in some cases prevent death following inoculation with a type A2 strain of F. tularensis. In contrast, inoculation of a type A1b strain was uniformly lethal in cottontail rabbits irrespective of a prior type B inoculation. These findings provide important insight about the role cottontail rabbits may play in environmental maintenance and transmission of this organism.

  5. Parasitic helminths of the wild rabbit, Oryctolagus cuniculus, in different bioclimatic zones in Tenerife, Canary Islands.

    Science.gov (United States)

    Foronda, P; Del Castillo, A; Abreu, N; Figueruelo, E; Piñero, J; Casanova, J C

    2003-12-01

    Faunistic and ecological analyses of the wild rabbit helminth fauna were undertaken in Tenerife island (Canary Islands). Rabbits were collected between 1998 and 2000 in seven bioclimatic zones in Tenerife selected by orientation and altitude. Five parasite species were identified, three cestodes (Taenia pisiformis (larvae), Andrya cuniculi and Mosgovoyia ctenoides) and two nematodes (Trichostrongylus retortaeformis and Passalurus ambiguus). Taenia pisiformis presented an irregular distribution with significant differences in prevalences between the zones. Andrya cuniculi was only found in two zones and there were no significant differences in prevalence values. Mosgovoyia ctenoides presented a wide distribution with significant prevalences, which were higher in northern compared to southern zones. Trichostrongylus retortaeformis was absent in the low southern zones of the island. Passalurus ambiguus was found in all zones with no significant difference in the prevalence of infection. The differences in prevalences are likely to be explained by abiotic factors in the case of T. retortaeformis, and by the absence of definitive and intermediate hosts in the case of T. pisiformis and A. cuniculi, respectively. All parasite species in Tenerife are common helminths in the Iberian Peninsula, from which their rabbit hosts originated. No significant differences were recorded in the mean intensities of infection of any of the parasite species identified.

  6. Study on the Prevention of Rabbit Hair from Loss

    Institute of Scientific and Technical Information of China (English)

    刘世春; 张华鹏; 姚穆

    2001-01-01

    A new method is presented to solve the problem of loss of rabbit hair by using ES fiber blending with rabbit hair. ES fiber is used to bond the rabbit hair to prevent the rabbit hair from losing after heat setting. The factors affecting hair loss are heat setting temperature, rabbit hair/ES fiber blend ratio, fabric heating setting, twistsof yarn, etc. Temperature of heat setting and ES fiber content are the two key factors This method has almost no detrimental effect on the coziness of the fabric, which is better than other hair loss prevention methods.

  7. Single- and multiple-dose pharmacokinetics of marbofloxacin after oral administration to rabbits.

    Science.gov (United States)

    Carpenter, James W; Pollock, Christal G; Koch, David E; Hunter, Robert P

    2009-04-01

    OBJECTIVE-To determine the pharmacokinetics of marbofloxacin after oral administration every 24 hours to rabbits during a 10-day period. ANIMALS-8 healthy 9-month-old female New Zealand White rabbits. PROCEDURES-Marbofloxacin (5 mg/kg) was administered orally every 24 hours to 8 rabbits for 10 days. The first day of administration was designated as day 1. Blood samples were obtained at 0, 0.17, 0.33, 0.5, 0.75, 1, 1.5, 2, 3, 4, 5, 6, 8, 12, and 24 hours on days 1 and 10 of marbofloxacin administration. Plasma marbofloxacin concentrations were quantitated by use of a validated liquid chromatography-mass spectrometry assay. Pharmacokinetic analysis of marbofloxacin was analyzed via noncompartmental methods. RESULTS-After oral administration, mean +/- SD area under the curve was 10.50 +/- 2.00 microg.h/mL and 10.90 +/- 2.45 microg.h/mL, maximum plasma concentration was 1.73 +/- 0.35 microg/mL and 2.56 +/- 0.71 microg/mL, and harmonic mean terminal half-life was 8.0 hours and 3.9 hours for days 0 and 10, respectively. CONCLUSIONS AND CLINICAL RELEVANCE-Marbofloxacin administered orally every 24 hours for 10 days appeared to be absorbed well and tolerated by rabbits. Administration of marbofloxacin at a dosage of 5 mg/kg, PO, every 24 hours is recommended for rabbits to control infections attributable to susceptible bacteria.

  8. Modulation of forelimb and hindlimb muscle activity during quadrupedal tied-belt and split-belt locomotion in intact cats.

    Science.gov (United States)

    Frigon, A; Thibaudier, Y; Hurteau, M-F

    2015-04-02

    The modulation of the neural output to forelimb and hindlimb muscles when the left and right sides step at different speeds from one another in quadrupeds was assessed by obtaining electromyography (EMG) in seven intact adult cats during split-belt locomotion. To determine if changes in EMG during split-belt locomotion were modulated according to the speed of the belt the limb was stepping on, values were compared to those obtained during tied-belt locomotion (equal left-right speeds) at matched speeds. Cats were chronically implanted for EMG, which was obtained from six muscles: biceps brachii, triceps brachii, flexor carpi ulnaris, sartorius, vastus lateralis and medial gastrocnemius. During tied-belt locomotion, cats stepped from 0.4 to 1.0m/s in 0.1m/s increments whereas during split-belt locomotion, cats stepped with left-right speed differences of 0.1 to 0.4m/s in 0.1m/s increments. During tied-belt locomotion, EMG burst durations and mean EMG amplitudes of all muscles respectively decreased and increased with increasing speed. During split-belt locomotion, there was a clear differential modulation of the EMG patterns between flexors and extensors and between the slow and fast sides. Changes in the EMG pattern of some muscles could be explained by the speed of the belt the limb was stepping on, while in other muscles there were clear dissociations from tied-belt values at matched speeds. Therefore, results show that EMG patterns during split-belt locomotion are modulated to meet task requirements partly via signals related to the stepping speed of the homonymous limb and from the other limbs.

  9. Safety belt usage in Finland and in other Nordic countries.

    NARCIS (Netherlands)

    Valtonen, J.

    1992-01-01

    Legislation has played a significant role in increasing safety belt usage in Finland and in the other Nordic countries. Publicity and enforcement have, however, been required to support the legislation. The development of safety belt regulations has been nearly similar in all these countries, both i

  10. Canadian seat belt wearing rates, promotion programs, and future directions.

    NARCIS (Netherlands)

    Grant, B.A.

    1992-01-01

    On the basis of a national driver seat belt survey conducted in Canada each year, the most important results are presented. A number of programmes for increasing seat belt use has been evaluated in Canada. Finally, a description is given of some of the current and planned activities within Canada wh

  11. Ambient Response Analysis of the Great Belt Bridge

    DEFF Research Database (Denmark)

    Brincker, Rune; Frandsen, J. B.; Andersen, P.

    2000-01-01

    In this paper an ambient response analysis of the Great Belt Bridge is presented. The Great Belt Bridge is one of the largest suspension bridges in the world, and the analysis was carried out in order to investigate the possibilities of estimating reliable damping values from the ambient response...

  12. Biomechanical considerations for abdominal loading by seat belt pretensioners.

    Science.gov (United States)

    Rouhana, Stephen W; El-Jawahri, Raed E; Laituri, Tony R

    2010-11-01

    While seat belts are the most effective safety technology in vehicles today, there are continual efforts in the industry to improve their ability to reduce the risk of injury. In this paper, seat belt pretensioners and current trends towards more powerful systems were reviewed and analyzed. These more powerful systems may be, among other things, systems that develop higher belt forces, systems that remove slack from belt webbing at higher retraction speeds, or both. The analysis started with validation of the Ford Human Body Finite Element Model for use in evaluation of abdominal belt loading by pretensioners. The model was then used to show that those studies, done with lap-only belts, can be used to establish injury metrics for tests done with lap-shoulder belts. Then, previously-performed PMHS studies were used to develop AIS 2+ and AIS 3+ injury risk curves for abdominal interaction with seat belts via logistic regression and reliability analysis with interval censoring. Finally, some considerations were developed for a possible laboratory test to evaluate higher-powered pretensioners.

  13. Policy Implications from an Evaluation of Seat Belt Use Regulation.

    Science.gov (United States)

    Desai, Anand; You, Min-Bong

    1992-01-01

    Effects of Ohio's mandatory seat belt law on seat belt use, number of car accidents, and number of fatal and severe injuries were evaluated for January 1982 through March 1988. The monthly average number of accident victims was 2,002. Implications for public policy formulation and implementation are discussed. (SLD)

  14. An Evaluation of the Seat Belt Education Campaign.

    Science.gov (United States)

    Rochon, James

    A seat belt education campaign conducted in Canada to dispel myths surrounding seat belts and promote a better understanding of their functions was evaluated. Two telephone surveys, each comprised of 4,000 respondents, were conducted. The first was done immediately before the campaign and the second immediately succeeding the campaign. Also, a…

  15. Teaching Taekwondo in Physical Education: Incorporating the Color Belt System

    Science.gov (United States)

    Oh, Hyun-Ju; Hannon, James C.; Banks, Aaron

    2006-01-01

    Taekwondo is an excellent lifetime physical activity that provides both physical and mental benefits to its participants. The color belt system may be creatively used in physical education to encourage improvement in all learning domains. This article provides information on incorporating the color belt system into physical education, and provides…

  16. HUMAN NEURONAL INTERLIMB COORDINATION DURING SPLIT-BELT LOCOMOTION

    NARCIS (Netherlands)

    DIETZ, [No Value; ZIJLSTRA, W; DUYSENS, J

    1994-01-01

    Human interlimb coordination and the adaptations in leg muscle activity were studied during walking on a treadmill with split belts. Four different belt speeds (0.5, 1.0, 1.5, 2.0 m/s) were offered in all possible combinations for the left and right leg. Subjects adapted automatically to a differenc

  17. Human neuronal interlimb coordination during split-belt locomotion

    NARCIS (Netherlands)

    Dietz, V.; Zijlstra, W.G.; Duysens, J.E.J.

    1994-01-01

    Human interlimb coordination and the adaptations in leg muscle activity were studied during walking on a treadmill with split belts. Four different belt speeds (0.5, 1.0, 1.5, 2.0 m/s) were offered in all possible combinations for the left and right leg. Subjects adapted automatically to a differenc

  18. Respiratory Belt Transducer Constructed Using a Singing Greeting Card Beeper

    Science.gov (United States)

    Bhaskar, Anand; Subramani, Selvam; Ojha, Rajdeep

    2013-01-01

    An article by Belusic and Zupancic described the construction of a finger pulse sensor using a singing greeting card beeper. These authors felt that this beeper made of piezoelectric material could be easily modified to function as a respiratory belt transducer to monitor respiratory movements. Commercially available respiratory belt transducers,…

  19. Proteomic analysis of regenerated rabbit lenses reveal crystallin expression characteristic of adult rabbits

    OpenAIRE

    Liu, Xialin; Min ZHANG; Liu, Yuhua; Challa, Pratap; Gonzalez, Pedro; Liu, Yizhi

    2008-01-01

    Purpose To explore lens crystallin characteristics and morphology of rabbit regenerated lenses in comparison with wild type natural lenses by means of proteomic analysis and histological assay. Methods The lens regeneration model of the New Zealand rabbit was established, and lens regeneration was observed by slit lamp examination and photography. A histological assay was evaluated under light microscopy and transmission electron microscopy (TEM). Protein samples of regenerated lenses were co...

  20. Towards a unique and transmissible vaccine against myxomatosis and rabbit haemorrhagic disease for rabbit populations

    OpenAIRE

    Angulo, Elena; Bárcena, Juan

    2007-01-01

    Currently available vaccines against myxomatosis and rabbit hemorrhagic disease virus (RHDV) are not suited to immunise wild rabbit populations, as vaccines need to be delivered individually by conventional veterinary practices. As an alternative approach, research in Spain has focused on the development of a transmissible vaccine. A recombinant virus has been constructed based on a naturally attenuated myxoma virus (MV) field strain, expressing the RHDV capsid protein (VP60). Following inocu...

  1. Landscape distribution characteristics of northern foothill belts of Tianshan Mountains

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The foothill belts of Tianshan Mountains are about 280 km long and 60 km wide, and the study area extends from Kuitun city to Fukang city. They are transitional belts between mountains and plains, appearing in three rows of folds with different morphologies and their age becoming younger from south to north. Based on GIS and RS methods, and materials of the previous researchers, this paper deals with the genetics of the foothill belts and their landscape features resulting from folding by neotectonic movements, and also describes their length, width and slope by remote sensing image interpretation. The characteristics of the foothill belts are found to be very important for the surrounding environment by preventing groundwater from flowing into plains, changing groundwater, increasing flow of surface runoff, in addition to their roles in protecting the surrounding environment. The purpose of this paper is to provide an in-depth understanding of the foothill belts and influence on its surrounding environment.

  2. Heads for shifting new generation of belt conveyors

    Energy Technology Data Exchange (ETDEWEB)

    Dembinski, M.

    1980-03-01

    New design of belt conveyors characterized by rigid section joints and conveyor to pontoon joints significantly influences loading of machines shifting belt conveyors to new locations. A new belt conveyor shifting machine head design, called SGP12 (selflocking head for vertical loads up to 120 kN) is described. Design is shown in 4 diagrams. It is stressed that in the SGP-12 the force clamping rollers on rails is automatically controlled depending on the height to which the rails are lifted. Clamping force is proportional to the height. This means of controlling the clamping force excludes damage to the head and reduces wear of the rollers. The SGP12 is, therefore, superior to the GP-12 shifting head of similar capacity. It is suggested that the SGP-12 is characterized by simple design, easier operation, higher reliability and greater safety. The SGP-12 can be used to shift belt conveyors with belts 2250 mm to 3000 mm wide.

  3. Detecting extrasolar asteroid belts through their microlensing signatures

    Science.gov (United States)

    Lake, Ethan; Zheng, Zheng; Dong, Subo

    2017-02-01

    We propose that extrasolar asteroid belts can be detected through their gravitational microlensing signatures. Asteroid belt + star lens systems create so-called 'pseudo-caustics', regions in the source plane where the magnification exhibits a finite but discontinuous jump. These features allow such systems to generate distinctive signatures in the microlensing light curves for a wide range of belt configurations, with source trajectories as far as tenths of the Einstein ring radius from the centre of the lens. Sample light curves for a range of asteroid belt parameters are presented. In the near future, space-based microlensing surveys like WFIRST, which will have the power of detecting per cent-level changes in microlensing light curves even with subminute exposure times, may be able to discover extrasolar asteroid belts with masses of the order of an earth mass.

  4. Experimental Measurements of Belt Gears in Newly Developed Device

    Directory of Open Access Journals (Sweden)

    Jozef Mascenik

    2016-05-01

    Full Text Available The paper deals with the alternative of determination of state of the belt gear. To realize themeasurements a newly developed device was designed for measurement and diagnostics of the belt gears. The main task is to detect the V-belt slip expressed by the coefficient of elastic creep and of specific slip with a measuring device. The measurements regarding can be performed if input revolutions of the electric motor and torque of the belt gear are constant whereas the tensioning force of the belt gear changes. It is also possible to perform the measurement if the input revolutions of the electric motor and the tensioning forces are constant and the torque changes.

  5. a Wave Model for a Pneumatic Tyre Belt

    Science.gov (United States)

    PINNINGTON, R. J.; BRISCOE, A. R.

    2002-06-01

    A one-dimensional wave equation of an infinite flattened tyre belt is generated. The belt vibration is controlled by bending, tension, shear and the sidewall stiffness. The dispersion relations for two waves in the belt are calculated and used to find both the input impedance and attenuation on a tyre belt of infinite extent. Tension and the sidewall controls the deformation and stiffness below 100Hz. Waves propagate around the belt above this frequency. The wave speeds due to bending and shear were predicted and measured. The model presented here should be valid for the prediction of tyre response above about 400 Hz when for a car tyre the modal behaviour is observed to cease. In this high-frequency region, the tyre at the input appears to be of infinite extent.

  6. Nonoperative management of pediatric aortic injury with seat belt syndrome.

    Science.gov (United States)

    Parrish, Dan W; Barnhorst, Amanda; Trebska-McGowan, Katarzyna; Amendola, Michael; Haynes, Jeffrey H

    2015-08-01

    "Seat belt syndrome" was first described by Garret and Braunstein in 1962. The syndrome involves skin and abdominal wall ecchymosis (seat belt sign) intra-abdominal solid organ and visceral injuries, as well as Chance fractures (compression and/or wedging deformity of the anterior portion of the vertebral body with disruption or fracture of the posterior elements, generally at L1-L3). We present a case of a 12-year-old male involved in a high-speed motor vehicle collision wearing only a lap belt resulting in seat belt syndrome, with disruption of the abdominal wall, mesenteric avulsion with multiple intestinal perforations, abdominal aortic dissection, and an L2 Chance fracture with cord transection. Intraoperative decision making is outlined with this scenario of complex injuries, and the literature of seat belt syndrome associated with blunt aortic injuries and its management is reviewed.

  7. Survey of current situation in radiation belt modeling

    Science.gov (United States)

    Fung, Shing F.

    2004-01-01

    The study of Earth's radiation belts is one of the oldest subjects in space physics. Despite the tremendous progress made in the last four decades, we still lack a complete understanding of the radiation belts in terms of their configurations, dynamics, and detailed physical accounts of their sources and sinks. The static nature of early empirical trapped radiation models, for examples, the NASA AP-8 and AE-8 models, renders those models inappropriate for predicting short-term radiation belt behaviors associated with geomagnetic storms and substorms. Due to incomplete data coverage, these models are also inaccurate at low altitudes (e.g., radiation data from modern space missions and advancement in physical modeling and data management techniques have now allowed the development of new empirical and physical radiation belt models. In this paper, we will review the status of modern radiation belt modeling. Published by Elsevier Ltd on behalf of COSPAR.

  8. Radiation-belt dynamics during solar minimum. Technical report

    Energy Technology Data Exchange (ETDEWEB)

    Gussenhoven, M.S.; Mullen, E.G.; Holeman, E.

    1989-12-01

    Two types of temporal variation in the radiation belts are studied using low altitude data taken onboard the DMSP F7 satellite: those associated with the solar cycle and those associated with large magnetic storm effects. Over a three-year period from 1984 to 1987 and encompassing solar minimum, the protons in the heart of the inner belt increased at a rate of approximately 6% per year. Over the same period, outer zone electron enhancements declined both in number and peak intensity. During the large magnetic storm of February 1986, following the period of peak ring current intensity, a second proton belt with energies up to 50 MeV was found at magnetic latitudes between 45 deg. and 55 deg. The belt lasted for more than 100 days. The slot region between the inner and outer electron belts collapsed by the merging of the two populations and did not reform for 40 days.

  9. A Study on the Vibration of the Charging Belt in an Electrostatic Accelerator

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The vibration of the charging belt in an electrostatic accelerator has intense influences on the accelerator operation. A calculating model was set up in this paper to study the belt vibration. The results show that the belt tension, belt velocity and belt current all contribute to the belt vibration. There is an optimal relationship among the three factors by which the belt would run most smoothly. There exists a minimum value of optimal tension for various belt velocities. The vibrating frequency of the is generally around several Hz.

  10. Encephalitozoonosis in household pet Nederland Dwarf rabbits (Oryctolagus cuniculus).

    Science.gov (United States)

    Valencakova, A; Balent, P; Petrovova, E; Novotny, F; Luptakova, L

    2008-05-31

    The paper presents the results of examination of 32 domestically bred rabbits, the breed Nederland Dwarf of Oryctolagus cuniculus, for the presence of Encephalitozoon cuniculi microsporidian species. The results of serological tests for E. cuniculi in 32 rabbits are reviewed along with other follow-up studies of clinical cases. Blood samples were taken from 7 asymptomatic rabbits and 25 rabbits showing neurological and ocular signs suggestive of encephalitozoonosis. In the asymptomatic group, 5 out of 7 rabbits were seropositive (71%). 16 rabbits with clinical diseases showed neurological sings, including torticollis, circus-like movements, loss of weight; 6 of them also showed ataxia, anorexia, asthenia of hind-limbs and 3 showed ocular signs. All 25 rabbits were seropositive. The spores of E. cuniculi were isolated from the faecal samples or kidneys and brain of an animal and subsequently were used for DNA isolation and PCR analysis.

  11. Strategies for rearing of rabbit does

    NARCIS (Netherlands)

    Rommers, J.M.

    2003-01-01

    This thesis describes the effects of different rearing strategies for young rabbit does on body development and reproduction performance. In current rearing, does are often fed to appetite from weaning to first insemination. First insemination is applied when 75 to 80% of mature body weight (BW) is

  12. Myxoma virus expressing interleukin-15 fails to cause lethal myxomatosis in European rabbits.

    Science.gov (United States)

    Liu, Jia; Wennier, Sonia; Reinhard, Mary; Roy, Edward; MacNeill, Amy; McFadden, Grant

    2009-06-01

    Myxoma virus (MYXV) is a poxvirus pathogenic only for European rabbits, but its permissiveness in human cancer cells gives it potential as an oncolytic virus. A recombinant MYXV expressing both the tdTomato red fluorescent protein and interleukin-15 (IL-15) (vMyx-IL-15-tdTr) was constructed. Cells infected with vMyx-IL-15-tdTr secreted bioactive IL-15 and had in vitro replication kinetics similar to that of wild-type MYXV. To determine the safety of this virus for future oncolytic studies, we tested its pathogenesis in European rabbits. In vivo, vMyx-IL-15-tdTr no longer causes lethal myxomatosis. Thus, ectopic IL-15 functions as an antiviral cytokine in vivo, and vMyx-IL-15-tdTr is a safe candidate for animal studies of oncolytic virotherapy.

  13. Equilibria and Free Vibration of a Two-Pulley Belt-Driven System with Belt Bending Stiffness

    Directory of Open Access Journals (Sweden)

    Jieyu Ding

    2014-01-01

    Full Text Available Nonlinear equilibrium curvatures and free vibration characteristics of a two-pulley belt-driven system with belt bending stiffness and a one-way clutch are investigated. With nonlinear dynamical tension, the transverse vibrations of the translating belt spans and the rotation motions of the pulleys and the accessory shaft are coupled. Therefore, nonlinear piecewise discrete-continuous governing equations are established. Considering the bending stiffness of the translating belt spans, the belt spans are modeled as axially moving beams. The pattern of equilibria is a nontrivial solution. Furthermore, the nontrivial equilibriums of the dynamical system are numerically determined by using two different approaches. The governing equations of the vibration near the equilibrium solutions are derived by introducing a coordinate transform. The natural frequencies of the dynamical systems are studied by using the Galerkin method with various truncations and the differential and integral quadrature methods. Moreover, the convergence of the Galerkin truncation is investigated. Numerical results reveal that the study needs 16 terms after truncation in order to determine the free vibration characteristics of the pulley-belt system with the belt bending stiffness. Furthermore, the first five natural frequencies are very sensitive to the bending stiffness of the translating belt.

  14. Evaluation of Positron Emission Tomographic Tracers for Imaging of Papillomavirus-Induced Tumors in Rabbits

    Directory of Open Access Journals (Sweden)

    Sonja Probst

    2014-01-01

    Full Text Available In this study, simultaneous positron emission tomography (PET/magnetic resonance (MR imaging was employed to evaluate the feasibility of the PET tracers 2-deoxy-2-18F-fluoro-D-glucose (18F-FDG, 11C-choline, and 18F-fluorothymidine (18F-FLT to detect papillomavirus-induced tumors in an established rabbit model system. The combined PET/MR allowed the analysis of tracer uptake of the tumors using the morphologic information acquired by MR. New Zealand White rabbits were infected with cottontail rabbit papillomavirus genomes and were imaged for up to 10 months with a simultaneous PET/MR system during the course of infection. The uptake characteristics of the PET tracers 11C-choline and 18F-FLT of tumors and reference tissues were examined relative to the clinical standard, 18F-FDG. Tracer biodistribution of various organs was measured by gamma-counting after the last PET scan and compared to the in vivo PET/MR 18F-FDG uptake. Increased tracer uptake was found 2 months postinfection in primary tumors with 18F-FDG and 11C-choline, whereas 18F-FLT failed to detect the tumors at all measured time points. Our data show that the PET tracer 18F-FDG is superior for imaging papillomavirus-induced tumors in rabbits compared to 11C-choline and 18F-FLT. However, 11C-choline imaging, which has previously been applied to detect various tumor entities in patients, appears to be an alternative to 18F-FDG.

  15. Inhibitory effects of prostaglandin E1 on activation of hepatic stellate cells in rabbits with schistosomiasis

    Institute of Scientific and Technical Information of China (English)

    Wei-Long Zou; Zhen Yang; Yun-Jin Zang; Dong-Jian Li; Zhi-Peng Liang; Zhong-Yang Shen

    2007-01-01

    BACKGROUND:Liver ifbrosis is the result of an imbalance between synthesis and degradation of extracellular matrix proteins of the liver. At the cellular and molecular levels, this progressive process is mainly characterized by activation of hepatic stellate cells (HSCs). Schistosoma japonica is one of the most prevalent causes of liver ifbrosis in China. It is characterized by hepatocyte damage, inlfammation, and chronic parasite egg-induced granuloma formation leading to ifbrosis. This study aimed to investigate the inhibitory effects of prostaglandin E1 (PGE1) on activation of HSCs and the alteration of type Ⅰ and Ⅲ collagen in rabbits with schistosomiasis. The study may promote the clinical application of praziquantel and PGE1 as a combined therapy to reverse hepatic ifbrosis caused by schistosomiasis. METHODS: Rabbits were percutaneously infected with cercaria of S. japonicum. Seven rabbits were subjected to intravenous injections of PGE1 (2.5 μg/kg daily) from days 60 to 120 after infection. The ultrastructural changes in activated HSCs were observed under transmission electron microscopy. The expression ofα-smooth muscle actin (α-SMA) was detected by immunohistochemistry. Fibril-forming collagens were detected by picrosirius staining. RESULTS: Activation of HSCs was a characteristic alteration in schistosome-induced hepatic ifbrosis. The expression of contraction-related α-SMA and the content of collagens were increased. Exogenous PGE1 markedly inhibited the activation of HSCs and reduced the expression of α-SMA around the hepatic sinusoids (P CONCLUSIONS:Activation of HSCs may play a key role in the progress of schistosome-induced hepatic ifbrosis. PGE1 effectively protects rabbit liver from ifbrosis, at least in part by inhibiting the activation of HSCs.

  16. The JCMT Gould Belt Survey: Understanding the influence of molecular outflows on Gould Belt clouds

    CERN Document Server

    Drabek-Maunder, E; Buckle, J V; Di Francesco, J; Richer, J

    2015-01-01

    Using JCMT Gould Belt Survey data from CO J=3-2 isotopologues, we present a meta-analysis of the outflows and energetics of star-forming regions in several Gould Belt clouds. The majority of the regions are strongly gravitationally bound. There is evidence that molecular outflows transport large quantities of momentum and energy. Outflow energies are at least 20 per cent of the total turbulent kinetic energies in all of the regions studied and greater than the turbulent energy in half of the regions. However, we find no evidence that outflows increase levels of turbulence, and there is no correlation between the outflow and turbulent energies. Even though outflows in some regions contribute significantly to maintaining turbulence levels against dissipation, this relies on outflows efficiently coupling to bulk motions. Other mechanisms (e.g. supernovae) must be the main drivers of turbulence in most if not all of these regions.

  17. Main-belt comets: sublimation-driven activity in the asteroid belt

    CERN Document Server

    Hsieh, Henry H

    2015-01-01

    Our knowledge of main-belt comets (MBCs), which exhibit comet-like activity likely due to the sublimation of volatile ices, yet orbit in the main asteroid belt, has increased greatly since the discovery of the first known MBC, 133P/Elst-Pizarro, in 1996, and their recognition as a new class of solar system objects after the discovery of two more MBCs in 2005. I review work that has been done over the last 10 years to improve our understanding of these enigmatic objects, including the development of systematic discovery methods and diagnostics for distinguishing MBCs from disrupted asteroids (which exhibit comet-like activity due to physical disruptions such as impacts or rotational destabilization). I also discuss efforts to understand the dynamical and thermal properties of these objects.

  18. A Century of Shope Papillomavirus in Museum Rabbit Specimens.

    Directory of Open Access Journals (Sweden)

    Clara Escudero Duch

    Full Text Available Sylvilagus floridanus Papillomavirus (SfPV causes growth of large horn-like tumors on rabbits. SfPV was described in cottontail rabbits (probably Sylvilagus floridanus from Kansas and Iowa by Richard Shope in 1933, and detected in S. audubonii in 2011. It is known almost exclusively from the US Midwest. We explored the University of Kansas Natural History Museum for historical museum specimens infected with SfPV, using molecular techniques, to assess if additional wild species host SfPV, and whether SfPV occurs throughout the host range, or just in the Midwest. Secondary aims were to detect distinct strains, and evidence for strain spatio-temporal specificity. We found 20 of 1395 rabbits in the KU collection SfPV symptomatic. Three of 17 lagomorph species (S. nuttallii, and the two known hosts were symptomatic, while Brachylagus, Lepus and eight additional Sylvilagus species were not. 13 symptomatic individuals were positive by molecular testing, including the first S. nuttallii detection. Prevalence of symptomatic individuals was significantly higher in Sylvilagus (1.8% than Lepus. Half of these specimens came from Kansas, though new molecular detections were obtained from Jalisco-Mexico's first-and Nebraska, Nevada, New Mexico, and Texas, USA. We document the oldest lab-confirmed case (Kansas, 1915, pre-dating Shope's first case. SfPV amplification was possible from 63.2% of symptomatic museum specimens. Using multiple methodologies, rolling circle amplification and, multiple isothermal displacement amplification in addition to PCR, greatly improved detection rates. Short sequences were obtained from six individuals for two genes. L1 gene sequences were identical to all previously detected sequences; E7 gene sequences, were more variable, yielding five distinct SfPV1 strains that differing by less than 2% from strains circulating in the Midwest and Mexico, between 1915 and 2005. Our results do not clarify whether strains are host species

  19. Crossbreeding effects on rabbit reproduction from four maternal lines of rabbits.

    Science.gov (United States)

    Ragab, M; Sánchez, J P; Mínguez, C; Baselga, M

    2016-07-01

    Litter size is essential for an efficient production of rabbit meat. A diallel cross between four maternal lines was carried out and the analysis of the components of litter size has been already done. This paper presents the analysis of litter size traits themselves (total born (TB), number born alive (NBA), number weaned (NW)) and kindling interval (KI), that complete the analysis of the reproductive performance. The 16 genetic groups were distributed in four Spanish farms. The V line was present in all farms in order to be used as reference group. A total of 34 546 parities from 7111 does, were analysed. The crossbreeding parameters were estimated according to Dickerson model. The differences between lines performance were of low magnitude and not significant for litter size traits. The LP line showed the shortest KI followed by H respect to lines A and V. These differences reflected the differences between direct and maternal genetic effects. The differences between the average of all crosses and line V were found to be significant and seemed to be important, being 0.46 for TB, 0.56 for NBA, 0.75 for NW and -2.21 days for KI. The differences between reciprocal crosses for litter size were of low magnitude and non-significant, which indicate that the maternal effects are not important between these lines. In general, the lines did not show significant differences in direct and maternal genetic effects for TB, NBA and NW but there were some significant differences for KI, which ranged from 1.54 to 6.85 days in direct effects and from 0.63 to 3.38 days for maternal effects. A positive and, in some cases, relevant heterosis was found. The largest heterosis was for TB in the HV cross (1.05 rabbits), followed by the AH (0.74 rabbits), AV (0.57 rabbits) and LH (0.55 rabbits) crosses. For NBA, significant heterosis was found in HV (1.11 rabbits) and AV (0.49 rabbits) and for NW in AV (0.90 rabbits), LH (0.70 rabbits) and LV (0.58 rabbits). Favourable and significant

  20. East Central Uplift Belt of Junggar Basin

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    @@ Mosuowandong ( Z3 ) and Dongdaohaizi (Z4) are two bidding blocks located in the east part of central uplift Belt, the hinterland of Junggar Basin. Xinjiang Uygur Autonomous Region. It totally covers an area of 8 100km2. Topographically, the two blocks are quite gentle with elevation of 380-400 m on average. The north part is desert and the south area is good for farming. There are three ephemeral streams flowing across the desert from south to north. The ground water is buried at the depth ranging from 6 to 8 m. It belongs to continental climate with the annually averaged precipitation of 80 mm. The traffic is rather convenient in the south part of both blocks. There are several sand-paved roads and two asphalt roads connected with the highway from Karamay to Urumqi City.

  1. Subsurface Meridional Circulation in the Active Belts

    CERN Document Server

    Hernandez, I Gonzalez; Hill, F; Howe, R; Komm, R

    2008-01-01

    Temporal variations of the subsurface meridional flow with the solar cycle have been reported by several authors. The measurements are typically averaged over periods of time during which surface magnetic activity existed in the regions were the velocities are calculated. The present work examines the possible contamination of these measurements due to the extra velocity fields associated with active regions plus the uncertainties in the data obtained where strong magnetic fields are present. We perform a systematic analysis of more than five years of GONG data and compare meridional flows obtained by ring-diagram analysis before and after removing the areas of strong magnetic field. The overall trend of increased amplitude of the meridional flow towards solar minimum remains after removal of large areas associated with surface activity. We also find residual circulation toward the active belts that persist even after the removal of the surface magnetic activity, suggesting the existence of a global pattern o...

  2. NASA's Radiation Belt Storm Probe Mission

    Science.gov (United States)

    Sibeck, David G.

    2011-01-01

    NASA's Radiation Belt Storm Probe (RBSP) mission, comprising two identically-instrumented spacecraft, is scheduled for launch in May 2012. In addition to identifying and quantifying the processes responsible for energizing, transporting, and removing energetic particles from the Earth's Van Allen radiation, the mission will determine the characteristics of the ring current and its effect upon the magnetosphere as a whole. The distances separating the two RBSP spacecraft will vary as they move along their 1000 km altitude x 5.8 RE geocentric orbits in order to enable the spacecraft to separate spatial from temporal effects, measure gradients that help identify particle sources, and determine the spatial extent of a wide array of phenomena. This talk explores the scientific objectives of the mission and the manner by which the mission has been tailored to achieve them.

  3. Ambient tremors in a collisional orogenic belt

    Science.gov (United States)

    Chuang, Lindsay Yuling; Chen, Kate Huihsuan; Wech, Aaron G.; Byrne, Timothy; Peng, Wei

    2014-01-01

    Deep-seated tectonic tremors have been regarded as an observation tied to interconnected fluids at depth, which have been well documented in worldwide subduction zones and transform faults but not in a collisional mountain belt. In this study we explore the general features of collisional tremors in Taiwan and discuss the possible generation mechanism. In the 4 year data, we find 231 ambient tremor episodes with durations ranging from 5 to 30 min. In addition to a coseismic slip-induced stress change from nearby major earthquake, increased tremor rate is also highly correlated with the active, normal faulting earthquake swarms at the shallower depth. Both the tremor and earthquake swarm activities are confined in a small, area where the high attenuation, high thermal anomaly, the boundary between high and low resistivity, and localized veins on the surfaces distributed, suggesting the involvement of fluids from metamorphic dehydration within the orogen.

  4. Evaluation of rabbit antibody response against 8 and 16 kDa recombinant subunits of antigen B fromEchinococcus granulosus

    Institute of Scientific and Technical Information of China (English)

    Jahangir Abdi; Bahram Kazemi; Mohammad Hasan Karimfar; Mohammad Bagher Rokni

    2012-01-01

    ABSTRACT Objective:To immunize rabbits with12 and16 kDa recombinant subunits of antigenB from Echinococcus granulosus (E. granulosus) and measuring polyclonal antibody and humoral immune response usingELISA and gel diffusion.Methods:Two mentioned antigens were cloned and expressed in expression vector and purified by affinity chromatography.Four young rabbits were selected and challenged intradermally with yielded recombinant antigens.Rabbits’ sera were collected post infection and were tested usingELISA and gel diffusion for polyclonal antibody detection10 days after last injection.Results:The specific antibody against the recombinant peptides was efficiently produced within4 weeks post infection.Conclusions:Produced recombinants proteins could induce the immune response of the rabbits successfully. This process might improve the clarification of diagnosis and vaccination as regards hydatidosis.

  5. The upregulation of pro-inflammatory cytokines in the rabbit uterus under the lipopolysaccaride-induced reversible immunoresponse state.

    Science.gov (United States)

    Liu, S J; Shi, Y; Liu, C; Zhang, M; Zuo, Z C; Zeng, C J; Zhou, G B; Xian, H; Song, T Z

    2017-01-01

    The reproductive organs are more likely to develop gram-negative bacterial infection than other internal organs because of direct access to the body surface. The objective of this study was (1) to provide a suitable intravenous injection dose of lipopolysaccharides (LPS) instead of gram-negative bacterial infection in order to induce a reversible immunoresponse state and (2) to examine the expression levels of pro-inflammatory cytokines in the uterus of rabbits while in an immunoresponse state. Two series of experiments were performed to accomplish these objectives. In the first series, 20 healthy New Zealand White female rabbits were divided into 5 homogeneous groups (n=4), and intravenously injected with 0, 0.5, 1, 2, or 4mg/kg body weight (BW) of LPS derived from Escherichia coli dissolved in 2ml of sterile saline (LPS carrier). The control group received only saline. The concentrations of IL-1β, IL-6, and TNF-α in serum and the white blood cell count changed with time after LPS stimulation, and certain doses of LPS led to the death of some rabbits. The results suggested that a dose of 0.5mg/kg of LPS induced a reversible immunoresponse state. In the second series, 4 rabbits were not injected (0h), 16 rabbits were injected with 0.5mg/kg LPS, and 16 rabbits in the control group were injected with 2ml of sterile saline. Tissues of the uterine horn, uterine body, and cervix from the 36 rabbits were collected at 0, 1.5, 3, 6, and 12h (n=4) postinjection for examination of the expression levels of IL-1β, IL-6, and TNF-α by quantitative real-time PCR (qRT-PCR). The results suggested that 0.5mg/kg of LPS upregulated the expression levels of IL-1β, IL-6 and TNF-α in the uterine body and uterine horn, and IL-6 in the cervix. In conclusion, the expression levels of IL-1β, IL-6 and TNF-α were upregulated in the uterus of rabbits under the reversible immunoresponse state induced by 0.5mg/kg of LPS-injection.

  6. Geometry, kinematics and evolution of the Tongbai orogenic belt

    Institute of Scientific and Technical Information of China (English)

    HUANG Shaoying; XU Bei; WANG Changqiu; ZHAN Sheng; DENG Rongjing

    2006-01-01

    The Tongbai orogenic belt (TOB) is composed of six tectonic units. From south to north these units are: Tongbai gneiss rise (TGR); Hongyihe-Luozhuang eclogite belt (HLE); Maopo-Hujiazhai igneous rock belt (MHI); Zhoujiawan flysch belt (ZFB); Yangzhuang greenschist belt (YGB); and Dongjiazhuang marble belt (DMB).The geometry and kinematic images of the TOB include: the antiformal structures caused by a later uplift process, the top-to-north ductile shear structure that related to a process that the ultrahigh pressure rocks are brought to surface, the top-to-south ductile shear thrust and the sinistrial shear structures related to a south-north direction compression, and the east-west direction fold structures in the upper crust. In the view of the multistage subduction-collision orogenic belt, according to the characters of petrology and its distribution, geometry, kinematics and structural chronology in these tectonic units, tectonic evolution of the TOB can be divided into four stages: oceanic crust subduction during 400-300 Ma, continental collision during 270-250 Ma, continental deep subduction and uplift during 250-205 Ma and doming deformation during 200-185 Ma.

  7. Selected HIV-1 Env trimeric formulations act as potent immunogens in a rabbit vaccination model.

    Directory of Open Access Journals (Sweden)

    Leo Heyndrickx

    Full Text Available BACKGROUND: Ten to 30% of HIV-1 infected subjects develop broadly neutralizing antibodies (bNAbs during chronic infection. We hypothesized that immunizing rabbits with viral envelope glycoproteins (Envs from these patients may induce bNAbs, when formulated as a trimeric protein and in the presence of an adjuvant. METHODS: Based on in vitro neutralizing activity in serum, patients with bNAbs were selected for cloning of their HIV-1 Env. Seven stable soluble trimeric gp140 proteins were generated from sequences derived from four adults and two children infected with either clade A or B HIV-1. From one of the clade A Envs both the monomeric and trimeric Env were produced for comparison. Rabbits were immunized with soluble gp120 or trimeric gp140 proteins in combination with the adjuvant dimethyl dioctadecyl ammonium/trehalose dibehenate (CAF01. Env binding in rabbit immune serum was determined using ELISAs based on gp120-IIIB protein. Neutralizing activity of IgG purified from rabbit immune sera was measured with the pseudovirus-TZMbl assay and a PBMC-based neutralization assay for selected experiments. RESULTS: It was initially established that gp140 trimers induce better antibody responses over gp120 monomers and that the adjuvant CAF01 was necessary for such strong responses. Gp140 trimers, based on HIV-1 variants from patients with bNAbs, were able to elicit both gp120IIIB specific IgG and NAbs to Tier 1 viruses of different subtypes. Potency of NAbs closely correlated with titers, and an gp120-binding IgG titer above a threshold of 100,000 was predictive of neutralization capability. Finally, peptide inhibition experiments showed that a large fraction of the neutralizing IgG was directed against the gp120 V3 region. CONCLUSIONS: Our results indicate that the strategy of reverse immunology based on selected Env sequences is promising when immunogens are delivered as stabilized trimers in CAF01 adjuvant and that the rabbit is a valuable model

  8. Resistance and other parameters of belt conveyor shifting

    Energy Technology Data Exchange (ETDEWEB)

    Dembinski, M.

    1988-07-01

    Discusses a method developed by Poltegor for calculating energy consumption of belt conveyor shifting in brown coal surface mines in Poland. A program for simulating effects of all significant factors that represent the environment, physical and mechanical properties of soil, effects of weather and atmospheric precipitations, design of belt conveyors, effects of soil or coal buildup and conveyor wear is used. Indices that describe effects of major groups of factors are used for calculating resistance to belt conveyor shifting considering local conditions. Investigations carried out in the Belchatow brown coal mine show the method to be accurate and reliable.

  9. North Florida is part of the stroke belt.

    OpenAIRE

    Siegel, P. Z.; Wolfe, L E; Wilcox, D.; Deeb, L C

    1992-01-01

    Florida is the only State in the southeastern United States that is not part of the "stroke belt." The National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health, defines the stroke belt as those States with an age-adjusted stroke mortality rate for the years 1979 to 1981 that is more than 10 percent above the national annual rate of 40.3 per 100,000 population. By reproducing at the county level the methods that were used at the State level to describe the stroke belt, ...

  10. Human neuronal interlimb coordination during split-belt locomotion

    OpenAIRE

    Dietz, V; Zijlstra, W G; Duysens, J.E.J.

    1994-01-01

    Human interlimb coordination and the adaptations in leg muscle activity were studied during walking on a treadmill with split belts. Four different belt speeds (0.5, 1.0, 1.5, 2.0 m/s) were offered in all possible combinations for the left and right leg. Subjects adapted automatically to a difference in belt speed within 10–20 stride cycles.This adaptation was achieved by a reorganization of the stride cycle with a relative shortening of the duration of the support and lengthening of the swin...

  11. Radiation Belt Storm Probes: Resolving Fundamental Physics with Practical Consequences

    Science.gov (United States)

    Ukhorskiy, Aleksandr Y.; Mauk, Barry H.; Fox, Nicola J.; Sibeck, David G.; Grebowsky, Joseph M.

    2011-01-01

    The fundamental processes that energize, transport, and cause the loss of charged particles operate throughout the universe at locations as diverse as magnetized planets, the solar wind, our Sun, and other stars. The same processes operate within our immediate environment, the Earth's radiation belts. The Radiation Belt Storm Probes (RBSP) mission will provide coordinated two-spacecraft observations to obtain understanding of these fundamental processes controlling the dynamic variability of the near-Earth radiation environment. In this paper we discuss some of the profound mysteries of the radiation belt physics that will be addressed by RBSP and briefly describe the mission and its goals.

  12. Power Transmission Through Timing Belt In Two Wheeler Motors

    Directory of Open Access Journals (Sweden)

    Gurumurthy Veerapathiran

    2015-03-01

    Full Text Available This paper studies the effect of noise and friction on performance of the chain drive system for motor bikes. Experiment shows that chain transmission in chain drive system leads to poor overall performance, due to its noise and chain gets loose due to aging and sprockets wear due to chain friction etc., the proposed system consists of drive and driven pulley with timing belt transmission. Compared to conventional method, proposed method give improved tension in pulleys and belt by the additional arrangement called belt tensioner. This gives good overall performance of the system, and reduces noise, vibration and gives high transmission speed.

  13. First reporting of methicillin-resistant Staphylococcus aureus (MRSA) ST398 in an industrial rabbit holding and in farm-related people.

    Science.gov (United States)

    Agnoletti, Fabrizio; Mazzolini, Elena; Bacchin, Cosetta; Bano, Luca; Berto, Giacomo; Rigoli, Roberto; Muffato, Giovanna; Coato, Paola; Tonon, Elena; Drigo, Ilenia

    2014-05-14

    Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has been described in food-producing animals and farm or slaughterhouse workers involved in the primary industrial production of swine, bovine and poultry. This communication describes the first case of LA-MRSA (ST398, spa types t034 and t5210) occurring in rabbits raised intensively for meat production and involving farm workers or their family members. In 2012-2013, in a study involving 40 rabbit industrial holdings in Italy, one farm was found to have rabbits colonized or infected with MRSA. Four farm workers and one of their relatives were found to be carrying MRSA. In this case holding, rabbits, people and the holding environment were further investigated and followed up by a second sampling five months later. MRSA was found in 48% (11/23) and 25% (15/59) of the rabbits carrying S. aureus at first and second samplings, respectively. Five months after first detection, some farm workers or family members were still MRSA carriers. Surface samples (2/10) and air samples (2/3) were contaminated with MRSA. Air samples yielded MRSA counts of 5 and 15CFU/m(3). MRSA from rabbits and people collected at first sampling were spa types t034 and t5210 belonging to ST398. The MRSA isolates from rabbits and persons tested at second sampling were t034 and t5210, but spa types t1190 and t2970 were also detected in MRSA isolates from rabbits. Tracing the epidemiological pattern earlier may prevent further spread of LA-MRSA in these food producing animals.

  14. Practical and medical aspects of the use of car seat belts.

    NARCIS (Netherlands)

    Edelman, A. & Kampen, L.T.B. van

    1974-01-01

    An analysis is made of the influence of use and non-use of seat belts on type and severity of injuries to drivers and of the influence of seat belt type on type and severity of injuries to drivers. Users of lap belts and users of 3-point belts enjoy a comparable and considerable reduction of injury

  15. 30 CFR 57.14131 - Seat belts for surface haulage trucks.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Seat belts for surface haulage trucks. 57.14131... and Equipment Safety Devices and Maintenance Requirements § 57.14131 Seat belts for surface haulage trucks. (a) Seat belts shall be provided and worn in haulage trucks. (b) Seat belts shall be...

  16. Rabbit lung injury induced by explosive decompression

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective: To study the mechanism of rabbit lunginjury caused by explosive decompression. Methods: A total of 42 rabbits and 10 rats were served as the experimental animals. A slow recompressiondecompression test and an explosive decompression test were applied to the animals, respectively. And the effects of the given tests on the animals were discussed. Results: The slow recompression-decompression did not cause an obvious lung injury, but the explosive decompression did cause lung injuries in different degrees. The greater the decompression range was, the shorter the decompression duration was, and the heavier the lung injuries were. Conclusions: Explosive decompression can cause a similar lung injury as shock wave does. The primary mechanical causes of the lung injury might be a tensile strain or stress in the alveolar wall and the pulmonary surface's impacts on the inside wall of the chest.

  17. Sex Difference in the Repolarization Currents of Rabbit Ventricular Cells

    Institute of Scientific and Technical Information of China (English)

    RUAN Yanfei; LIU Nian; ZHOU Qiang; LI Yang; WANG Lin

    2005-01-01

    Summary: The current difference between male and female rabbit ventricular myocytes was investigated for elucidating the mechanism of longer QT interval and higher incidence of drug-associated torsade de pointes in female rabbits than in male rabbits. Whole cell patch clamp technique was used to record APD, Ito, IK,tail, IK1 and ICa,L of myocytes from left ventricular apex. There was no difference in the membrane capacitance between male and female rabbit myocytes. APD90 was longer in female rabbits (560.4±26.5 ms, n=15) than in male ones (489.0±20.7 ms, n=14), P0.05). The lower IK,tail of female rabbit myocytes may contribute to the longer repolarization and the higher incidence of drug-associated torsade de pointes.

  18. Trio of Neptunes and their Belt

    Science.gov (United States)

    2006-05-01

    Using the ultra-precise HARPS spectrograph on ESO's 3.6-m telescope at La Silla (Chile), a team of European astronomers have discovered that a nearby star is host to three Neptune-mass planets. The innermost planet is most probably rocky, while the outermost is the first known Neptune-mass planet to reside in the habitable zone. This unique system is likely further enriched by an asteroid belt. ESO PR Photo 18a/06 ESO PR Photo 18a/06 Planetary System Around HD 69830 (Artist's Impression) "For the first time, we have discovered a planetary system composed of several Neptune-mass planets", said Christophe Lovis, from the Geneva Observatory and lead-author of the paper presenting the results [1]. During more than two years, the astronomers carefully studied HD 69830, a rather inconspicuous nearby star slightly less massive than the Sun. Located 41 light-years away towards the constellation of Puppis (the Stern), it is, with a visual magnitude of 5.95, just visible with the unaided eye. The astronomers' precise radial-velocity measurements [2] allowed them to discover the presence of three tiny companions orbiting their parent star in 8.67, 31.6 and 197 days. "Only ESO's HARPS instrument installed at the La Silla Observatory, Chile, made it possible to uncover these planets", said Michel Mayor, also from Geneva Observatory, and HARPS Principal Investigator. "Without any doubt, it is presently the world's most precise planet-hunting machine" [3]. ESO PR Photo 18d/06 ESO PR Photo 18d/06 Phase Folded Measurements of HD 69830 The detected velocity variations are between 2 and 3 metres per second, corresponding to about 9 km/h! That's the speed of a person walking briskly. Such tiny signals could not have been distinguished from 'simple noise' by most of today's available spectrographs. The newly found planets have minimum masses between 10 and 18 times the mass of the Earth. Extensive theoretical simulations favour an essentially rocky composition for the inner planet, and

  19. Habitat Suitability Index Models: Swamp rabbit

    Science.gov (United States)

    Allen, Arthur W.

    1985-01-01

    A review and synthesis of existing information were used to develop a Habitat Suitability Index (HSI) model for the swamp rabbit (Sylvilagus aquaticus). The model consolidates habitat use information into a framework appropriate for field application, and is scaled to produce an index between 0.0 (unsuitable habitat) to 1.0 (optimum habitat). HSI models are designed to be used with Habitat Evaluation Procedures previously developed by the U.S. Fish and Wildlife Service.

  20. FORMATION AND EVOLUTION OF THE CENOZOIC THRUST FOLD BELT IN JINPING, SICHUAN

    Institute of Scientific and Technical Information of China (English)

    LIAO Zhongli; DENG Yongfu; LIAO Guangyu

    2003-01-01

    The Jinping orogenic belt in Sichuan, China consists mainly of the Jinpingshan intracontinental thrust-nappe belt, foreland thrust-nappe belt and foreland uplift belt. Based on analyses about the characteristics of the structural units in this area, the authors propose in this paper that Chapuzi-Bazhe revival fault belt is the regional boundary fault, and points out that after the formation of the Pre-Sinian basement, the western edge of the Yangtze paraplatform was turned into the passive continental margin in Sinian to Triassic, then into the Mesozoic collision orogenic belt, and finally into the Cenozoic orogenic belt through intracontinental orogeny.

  1. Tissue-specific transcription profile of cytokine and chemokine genes associated with flavivirus control and non-lethal neuropathogenesis in rabbits.

    Science.gov (United States)

    Suen, Willy W; Uddin, Muhammad Jasim; Prow, Natalie A; Bowen, Richard A; Hall, Roy A; Bielefeldt-Ohmann, Helle

    2016-07-01

    We previously showed that New Zealand White (NZWRs) and cottontail rabbits (CTRs) are a suitable model for studying immune mechanisms behind virus control and non-lethal neuropathogenesis associated with West Nile virus (WNV) and Murray Valley encephalitis virus (MVEV) infections. In the current study, we observed that MVEV infection induced high IFNα, TNFα, IL6, and CXCL10 transcript levels in the brains of weanling NZWRs, unlike infection with the less virulent WNVNSW2011. These transcript levels also correlated with encephalitis severity. Widespread STAT1 protein expression in brain with moderate neuropathology suggests that IFN-I signaling is crucial for limiting neural infection and mediating non-lethal neuropathogenesis. Unlike NZWRs, CTRs limit neuroinvasion without upregulation of many cytokine/chemokine transcripts, suggesting a species-dependent virus control mechanism. However, the common IFNγ, TNFα and IL6 transcript upregulation in specific lymphoid organs suggest some conserved elements in the response against flaviviruses, unique to all rabbits.

  2. Inhalational anthrax (Ames aerosol) in naïve and vaccinated New Zealand rabbits: characterizing the spread of bacteria from lung deposition to bacteremia.

    Science.gov (United States)

    Gutting, Bradford W; Nichols, Tonya L; Channel, Stephen R; Gearhart, Jeffery M; Andrews, George A; Berger, Alan E; Mackie, Ryan S; Watson, Brent J; Taft, Sarah C; Overheim, Katie A; Sherwood, Robert L

    2012-01-01

    There is a need to better understand inhalational anthrax in relevant animal models. This understanding could aid risk assessment, help define therapeutic windows, and provide a better understanding of disease. The aim here was to characterize and quantify bacterial deposition and dissemination in rabbits following exposure to single high aerosol dose (> 100 LD(50)) of Bacillus anthracis (Ames) spores immediately following exposure through 36 h. The primary goal of collecting the data was to support investigators in developing computational models of inhalational anthrax disease. Rabbits were vaccinated prior to exposure with the human vaccine (Anthrax Vaccine Adsorbed, AVA) or were sham-vaccinated, and were then exposed in pairs (one sham and one AVA) so disease kinetics could be characterized in equally-dosed hosts where one group is fully protected and is able to clear the infection (AVA-vaccinated), while the other is susceptible to disease, in which case the bacteria are able to escape containment and replicate uncontrolled (sham-vaccinated rabbits). Between 4-5% of the presented aerosol dose was retained in the lung of sham- and AVA-vaccinated rabbits as measured by dilution plate analysis of homogenized lung tissue or bronchoalveolar lavage (BAL) fluid. After 6 and 36 h, >80% and >96%, respectively, of the deposited spores were no longer detected in BAL, with no detectable difference between sham- or AVA-vaccinated rabbits. Thereafter, differences between the two groups became noticeable. In sham-vaccinated rabbits the bacteria were detected in the tracheobronchial lymph nodes (TBLN) 12 h post-exposure and in the circulation at 24 h, a time point which was also associated with dramatic increases in vegetative CFU in the lung tissue of some animals. In all sham-vaccinated rabbits, bacteria increased in both TBLN and blood through 36 h at which point in time some rabbits succumbed to disease. In contrast, AVA-vaccinated rabbits showed small numbers of CFU in

  3. Nerve growth factor enhances sleep in rabbits.

    Science.gov (United States)

    Takahashi, S; Krueger, J M

    1999-04-02

    Nerve growth factor (NGF) elicits rapid-eye-movement sleep (REMS) in cats. Removal of NGF receptor-positive cholinergic basal forebrain neurons inhibits REMS in rats. The aim of the present study was to determine the effects of NGF on sleep and brain temperature (Tbr) in rabbits. Male rabbits were implanted with electroencephalograph (EEG) electrodes, a brain thermistor and an intraventricular (i.c.v.) guide cannula. Rabbits received human beta-NGF i.c.v. (0.01, 0.1, 1.0 or 10 microg] and on a separate day, 25 microl pyrogen-free saline i.c.v. as control. EEG and Tbr were recorded for 23 h after injections. The highest two doses of NGF increased both non-REMS and REMS across the 23-h recording period. REMS was enhanced dose-dependently. Tbr was not affected by any dose of NGF. These results suggest that NGF is involved in both REMS and non-REMS regulation.

  4. Milkweed control by food imprinted rabbits.

    Science.gov (United States)

    Ducs, Anita; Kazi, Andrea; Bilkó, Ágnes; Altbäcker, Vilmos

    2016-09-01

    Many species of invasive plants are spreading out rapidly in Europe. The common milkweed occupies increasingly more area. Being poisonous, most animals will not graze on it however rabbits would be an effective organism for the biological control of milkweed. Rabbit kittens can learn the maternal diet in various ways. They prefer aromatic foods which their mother had eaten during pregnancy or lactation period, -even if it is poisonous- but they can also learn the maternal diet from the fecal pellets deposited by the mother into the nest during the nursing events. The present study was aimed to investigate if rabbit kittens can learn that the common milkweed is a potential food also. In the first 10days of their lives kits got fecal pellets originating from individuals having fed on common milkweed previously. When weaned on day 28 postpartum, these pups preferred the milkweed in the 3-way food choice test, opposite to the control group. Most surprisingly in a second experiment it was also shown that the common milkweed was also preferred by the kittens if their mother ate it not during, but one month before pregnancy.

  5. Reproductive activity and welfare of rabbit does

    Directory of Open Access Journals (Sweden)

    C. Castellini

    2010-04-01

    Full Text Available This paper reviews the relationships between reproductive performance and welfare of the rabbit does. In the last 10 years the profitability of rabbit farms has increased mainly due to improvements in management and genetic selection but several problems mainly related to animal welfare have also occurred. The mortality and rates of female replacement per year are very high and the replaced females often show poor body condition and low performance. The effect of kindling order, litter size, genetic strain, weaning age and reproduction rhythm on the reproductive performance and welfare of females and some mechanisms implicated in these effects are discussed. Modern rabbit does produce a lot of milk which have a high energetic value which leads to a mobilization of body fat which results in an energy deficit. In the current reproductive rhythms, there is an extensive overlap between lactation and gestation. The resulting energetic and hormonal antagonism reduces the fertility rate and lifespan of the doe. Strategies to improve the fertility, lifespan and welfare of does are discussed. An approach which combines various strategies seems to be required to meet these objectives. Since the factors involved in this productive system are fixed (genetic strain, environment the most powerful way to improve doe welfare is to choose a reproductive rhythm that is adapted to the physiology of the does.

  6. Bacteriocin-producing Enterococci from Rabbit Meat

    Directory of Open Access Journals (Sweden)

    Szabóová, R.

    2012-01-01

    Full Text Available Aims: Enterococci are lactic acid bacteria belonging to the division Firmicutes. They occur in different ecosystems, rabbits including. Enterococci can possess probiotic properties and produce antimicrobial substances-bacteriocins. Rabbit meat as nutritionally healthy food offers novel source to study bacteriocin-producing and/or probiotic enterococci. Methodology and results: Enterococci were detected from rabbit meat samples (42. Most of the isolates were allotted to the species Enterococcus faecium by PCR method. The isolates have possessed the structural genes for enterocins A, P, B production. The inhibitory substances produced by the isolated enterococci inhibited the growth of 12 indicators. Of 34 isolates, 15 strains have shown the antimicrobial activity against L. monocytogenes CCM 4699, 12 strains against S. aureus 3A3, 10 strains against S. aureus 5A2 as well as Salmonella enterica serovar Enteritidis PT4. Moreover, enterococci have tolerated 5 % bile, low pH; they have produced lactid acid in the amount from 0.740 ± 0.091 to 1.720 ± 0.095 mmol/l. The isolates were mostly sensitive to antibiotics. Conclusion, significance and impact of study: Bacteriocin-producing strain E. faecium M3a has been selected for more detail characterization of its bacteriocin and probiotic properties with the aim for its further application as an additive.

  7. Transgenic rabbit that expresses a functional human lipoprotein (a)

    Science.gov (United States)

    Rouy, Didier; Duverger, Nicolas; Emmanuel, Florence; Denefle, Patrice; Houdebine, Louis-Marie; Viglietta, Celine; Rubin, Edward M.; Hughes, Steven D.

    2003-01-01

    A transgenic rabbit which has in its genomic DNA sequences that encode apolipoprotein (a) and apolipoprotein B polypeptides which are capable of combining to produce lipoprotein (a), a process for creating such a rabbit, and the use of the rabbit to identify compounds which are effective in the treatment of human diseases which are associated with, induced and/or exacerbated by Lp(a) expression.

  8. The rabbit pup, a natural model of nursing anticipatory activity

    OpenAIRE

    Caba, Mario; González-Mariscal, Gabriela

    2009-01-01

    Mother rabbits nurse their young once a day with circadian periodicity. Nursing bouts are brief (ca. 3 min) and occur inside the maternal burrow. Despite this limited contact mother rabbits and their pups are tuned to each other to ensure that the capacities of each party are used efficiently to ensure the weaning of a healthy litter. In this review we present behavioral, hormonal, metabolic and hormonal correlates of this phenomenon in mother rabbits and their pups. Research is revealing tha...

  9. Medetomidine--ketamine--diazepam anesthesia in the rabbit.

    Science.gov (United States)

    Mero, M; Vainionpää, S; Vasenius, J; Vihtonen, K; Rokkanen, P

    1989-01-01

    Orthopaedic operations were performed in 340 rabbits when developing biodegradable rods for the fixation of fractures. The rabbits were anaesthesized by injecting medetomidine combined with ketamine and diazepam (MKD) subcutaneously. The mortality due to the anaesthesia was zero. Arterial blood gas analyses showed a moderate decrease in the haemoglobin oxygen saturation and compensated respiratory acidosis, which was well tolerated by the animals. It is concluded that MKD anaesthesia is well suited for orthopaedic operations in the rabbit.

  10. Toxicity of staphylococcal toxic shock syndrome toxin 1 in rabbits.

    OpenAIRE

    de Azavedo, J C; Arbuthnott, J P

    1984-01-01

    Strains of Staphylococcus aureus associated with toxic shock syndrome produce toxic shock syndrome toxin 1 (TSST 1), which is lethal to conventional rabbits and acts synergistically with gram-negative lipopolysaccharide. The lethal effect of TSST 1 was examined in specific-pathogen-free rabbits on the basis that these rabbits, being less colonized by gram-negative bacteria, would be less susceptible than conventional animals. Although there was no significant difference in mortality between s...

  11. CRED REA Fish Team Belt Transect Survey at Laysan 2004

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 3 sites at Laysan in the Northwest...

  12. Periumbilical allergic contact dermatitis: blue jeans or belt buckles?

    Science.gov (United States)

    Byer, Tara T; Morrell, Dean S

    2004-01-01

    Nickel is the most ubiquitous contact allergen among children and adolescents. Metal blue jeans buttons and belts have been noted to cause nickel dermatitis around the umbilicus. For these children, traditional teaching is strict avoidance of all pants with metal snaps/buttons, particularly blue jeans. In this study we tested 90 pairs of blue jeans and 47 belts for nickel using the dimethylglyoxime spot test. Only 10% of blue jeans tested positive, while 53% of belts tested positive. Furthermore, 10 pairs of nickel-negative blue jeans remained negative after 10 washings. Overall we found no resistance to testing in clothing stores. From these results, we recommend that patients with allergic contact dermatitis secondary to nickel need not strictly avoid blue jeans and metal belt buckles. Rather, families should be encouraged to use the dimethylglyoxime spot test to test these items for nickel prior to purchase.

  13. Back belts in occupational setting: what are the evidences?

    Directory of Open Access Journals (Sweden)

    João Marcos Bernardes

    2012-01-01

    Full Text Available Although, widely used the mechanisms of action and the effectiveness of back belts in the prevention of occupational low back disorders remains uncertain, generating controversy about its use. Therefore, the purpose of this study was to do a critical literature review about back belts use in the prevention of occupational low back pain in the occupational setting. In order to do that, a bibliographic research in the following data basis: Medline, SciELO and LILACS was conducted. There is evidence that back belts use reduces spine range of motion, however the studies about intra-abdominal pressure and muscle activity presented contradictory results, as the epidemiologic studies, not allowing the recommendation of its use in the prevention of occupational low back pain. Based on this literature review it is suggested therefore, the implementation of new studies, to confirm or not the effectiveness of back belts as personal protective equipment, in the prevention of occupational low back disorders.

  14. "Abomination"--life as a Bible belt gay.

    Science.gov (United States)

    Barton, Bernadette

    2010-01-01

    Drawing on observation, autoethnography, and audio-taped interviews, this article explores the religious backgrounds and experiences of Bible Belt gays. In the Bible Belt, Christianity is not confined to Sunday worship. Christian crosses, messages, paraphernalia, music, news, and attitudes permeate everyday settings. Consequently, Christian fundamentalist dogma about homosexuality-that homosexuals are bad, diseased, perverse, sinful, other, and inferior-is cumulatively bolstered within a variety of other social institutions and environments in the Bible Belt. Of the 46 lesbians and gay men interviewed for this study (age 18-74 years), most describe living through spirit-crushing experiences of isolation, abuse, and self-loathing. This article argues that the geographic region of the Bible Belt intersects with religious-based homophobia. Informants explained that negative social attitudes about homosexuality caused a range of harmful consequences in their lives including the fear of going to hell, depression, low self-esteem, and feelings of worthlessness.

  15. 5m Main Belt Asteroid Population Estimation Using Vesta Imagery

    Science.gov (United States)

    Rynders, Michael; Trilling, David E.

    2016-10-01

    The Main Belt is the largest source of Near-Earth asteroids, but objects 2 pixels in diameter that were counted in a 33km 2 region to give a crater density. By knowing the crater density and making some reasonable assumptions about the orbital distribution of asteroids and the age of Vesta's surface, an estimate of the population of small asteroids in the inner main belt was made. It was found that the inner region of the main asteroid belt contains approximately 20 billion asteroids larger than 5 m. These results agree well with the measured inner Main Belt Size distribution derived by the Wide-field Infrared Survey Explorer, WISE (Masiero et al. 2011).

  16. Jupiter's radiation belt ions - A comparison of theory and observation

    Science.gov (United States)

    Summers, Danny; Thorne, Richard M.; Mei, YI

    1989-01-01

    Radial profiles are constructed for the Jovian radiation belt flux-tube content Y-asterisk from the reported phase-space density of energetic particles obtained from Voyager 1 data over the range L = 6 to L = 9. These experimental profiles are compared with theoretical solutions for Y-asterisk from an interchange-diffusion model of the coupled radiation belt and Iogenic ion populations. Subject to certain limitations of the Voyager 1 data, the model solutions are found to be consistent with the data for a variety of input parameters. Model solutions are also found corresponding to radiation belt ions that are expected to be mainly responsible for the auroral energy input. Comparison of the present theoretical profiles with the data implies that the energetic radiation belt ions should have a peak loss rate within a factor of three of that for strong diffusion scattering.

  17. Cenozoic intracontinental deformation of the Kopeh Dagh Belt, Northeastern Iran

    Science.gov (United States)

    Chu, Yang; Wan, Bo; Chen, Ling; Talebian, Morteza

    2016-04-01

    Compressional intracontinental orogens represent large tectonic zones far from plate boundaries. Since intracontinental mountain belts cannot be framed in the conventional plate tectonics theory, several hypotheses have been proposed to account for the formations of these mountain belts. The far-field effect of collision/subduction at plate margins is now well accepted for the origin and evolution of the intracontinental crust thickening, as exemplified by the Miocene tectonics of central Asia. In northern Iran, the Binalud-Alborz mountain belt witnessed the Triassic tectonothermal events (Cimmerian orogeny), which are interpreted as the result of the Paleotethys Ocean closure between the Eurasia and Central Iran blocks. The Kopeh Dagh Belt, located to the north of the Binalud-Alborz Belt, has experienced two significant tectonic phases: (1) Jurassic to Eocene rifting with more than 7 km of sediments; and (2) Late Eocene-Early Oligocene to Quaternary continuous compression. Due to the high seismicity, deformation associated with earthquakes has received more and more attention; however, the deformation pattern and architecture of this range remain poorly understood. Detailed field observations on the Cenozoic deformation indicate that the Kopeh Dagh Belt can be divided into a western zone and an eastern zone, separated by a series of dextral strike-slip faults, i.e. the Bakharden-Quchan Fault System. The eastern zone characterized by km-scale box-fold structures, associated with southwest-dipping reverse faults and top-to-the NE kinematics. In contrast, the western zone shows top-to-the SW kinematics, and the deformation intensifies from NE to SW. In the northern part of this zone, large-scale asymmetrical anticlines exhibit SW-directed vergence with subordinate thrusts and folds, whereas symmetrical anticlines are observed in the southern part. In regard to its tectonic feature, the Kopeh Dagh Belt is a typical Cenozoic intracontinental belt without ophiolites or

  18. Emergence of new virulent rabbit hemorrhagic disease virus strains in Saudi Arabia.

    Science.gov (United States)

    Ismail, Mahmoud M; Mohamed, Mahmoud H A; El-Sabagh, Ibrahim M; Al-Hammadi, Mohamed A

    2017-02-01

    Rabbit hemorrhagic disease is an acute fatal highly contagious viral infectious disease that causes high losses among rabbitries. The disease was first reported in China in 1984 and later on in Saudi Arabia in 1996. The aim of this study was to investigate the emergence and pathogenicity of new rabbit hemorrhagic disease virus (RHDV) strains in Saudi Arabia. The pathogenicity was confirmed by inoculation in susceptible rabbits. Three RHDV strains were detected by reverse transcriptase polymerase chain reaction (RT-PCR) using primers targeting VP60 capsid protein gene in infected rabbitries during 2012 and 2013. These strains clustered into two genetically distinct genogroups related to year of isolation (G2 and G3). All new Saudi Arabia viruses clustered with the European strains, while the old strains clustered with strains from China and America. Based on amino acids and nucleotide sequences, the Saudi Arabia strains (RHD/1/SA/2012, RHD/2/SA/2012, and RHD/3/SA /2013) had high identity with Mexico89, Ca11-ITA, and 00-13,FRA virus; on the other hand, there was a relatively high identity with Bahrain strain. The evolutionary relationship of Saudi RHDVs strains revealed significant nucleotides and amino acid substitutions in hypervariable region E, suggesting the emergence of new RHDVs circulating in Saudi Arabia rabbitries. These antigenic changes represented by the antigenic index might be a potential cause of vaccination failure and raises the need to review the vaccination strategies against RHD.

  19. Design of MGA trajectories for main belt asteroid

    Institute of Scientific and Technical Information of China (English)

    崔祜涛; 乔栋; 崔平远; 栾恩杰

    2003-01-01

    Asteroid exploration is one of the most sophisticated missions currently being investigated. Gravityassist trajectories have proven valuable in interplanetary missions such as the Pioneer, Voyager and Galileo. In this paper, we design interplanetary trajectory for main belt asteroid exploration mission with the Mars gravityassist (MGA) using "pork chop" plots and patched-conic theory and give some initial valuable trajectory parameters on main belt asteroid exploration mission with MGA.

  20. The Role of Plasma in Radiation Belt Loss.

    Science.gov (United States)

    Jahn, J. M.; Bonnell, J. W.; Kurth, W. S.; Millan, R. M.; Goldstein, J.; Jaynes, A. N.; Blake, J. B.; Denton, R. E.

    2015-12-01

    The radiation belts are zones of relativistic electrons encircling the Earth. Their radial structure is controlled by the competition between source and loss processes. Most commonly, a two-belt structure prevails, though a more complicated three-belt structure - an inner belt plus two outer electron belts - have repeatedly been observed. The plasma conditions that enable and enhance loss-facilitating wave activity in the inner magnetosphere are still under discussion. Relativistic electrons have been thought to more easily resonate with electromagnetic ion-cyclotron waves (EMIC) when the total plasma density is large (i.e., in the plasmasphere and plume). However, there is evidence that this interaction may be not as strong as thought, and that instead the field-aligned motion of lower energy ring current ions (up to a few 10's keV) may play a key role. Similarly, the exact influence of large heavy ion (O+) concentrations remains unsettled. We use 2.5+ years of Van Allen Probes observations to study the region of plasmasphere-outer belt overlap (and its vicinity). By now, the Van Allen Probes provide a complete and very dense coverage of the complete magnetosphere inside geosynchronous orbit We focus our interest on understanding the plasma conditions that can favor EMIC wave growth. We investigate the temperature anisotropy A (modified by plasma β) of the warm/hot plasma, and contrast it with the location specifics of the plasmasphere (i.e., very high total density) and the occurrence of high O+ concentrations in the overlap regions with the radiation belt(s). We present both average conditions for all parameters during a variety of geomagnetic conditions, and highlight specific loss and overlap events in an effort to establish favorable plasma conditions for relativistic electron loss during those times.

  1. Rapid depletion of marbofloxacin residues in rabbit after therapeutic treatment.

    Science.gov (United States)

    Ligabue, Matteo; Lucchetti, Dario; Catone, Tiziana; Fabrizi, Laura; Marvasi, Luigi; Zaghini, Anna; Coni, Ettore

    2005-11-01

    Although rabbit meat production represents a very small percentage of the world meat market, this percentage has been growing continuously during the last 30 years. Rabbit is considered a minor food species, and therefore no drugs are specifically registered for this animal. This situation encourages rabbit farmers to make off-label use of antibacterial drugs authorized for food-producing animal species other than rabbits. In the present study, the distribution and elimination of the fluoroquinolone antibacterial agent marbofloxacin in rabbit muscle, liver, and kidney was investigated. Marbofloxacin was chosen as a representative of a new generation of antibacterial drugs active against most gram-positive and gram-negative bacteria and mycoplasms; it is well tolerated and has short elimination times in bovine and swine species. Rabbits were treated with marbofloxacin at 2 mg kg of body weight(-1) for 5 days. Residual concentrations in liver, kidney, and muscle tissues were determined posttreatment with high-performance liquid chromatography and fluorescence detection. Marbofloxacin was rapidly distributed and eliminated from rabbit tissues. Concentrations were higher in the liver and kidney than in muscle. However, 48 h after the end of treatment, marbofloxacin concentrations dropped below the maximum residue level fixed for this antibacterial drug in cattle and pigs. Considering the efficacy of marbofloxacin for the treatment of the most common rabbit diseases, its tolerability, and its short elimination time as verified in the present study, use of this antibacterial drug could be extended to therapeutic treatment of rabbits.

  2. Fattening and slaughter traits of four rabbit genotypes

    OpenAIRE

    Silvester Zgur; Ajda Kermauner

    2010-01-01

    Growth rate of 4 genotypes, two lines of Slovene SIKA meat rabbit (maternal line A and sire line C, both 50 rabbits), hybrid AxC (50 rabbits) and crossbred AxCal (A and Californian, 38 rabbits) was recorded from weaning (35 days of age) to slaughter (93 days). Growth performance and slaughter traits of AxC were different from line A (live weight at 91st day: A 2720, C 3158, AxC 3043 g (P£0.05), heterosis effect (H) +3.38%; carcass weight: A 1490, C 1787, AxC 1716 g (P£0.05), H +5....

  3. Abstracts of the 27th Hungarian conference on rabbit production

    Directory of Open Access Journals (Sweden)

    Abstracts, Conferences, Congresses, Symposiums...

    2015-12-01

    Full Text Available About 100 participants took part in the 27th Hungarian Conference on Rabbit Production at Kaposvár, organised by the Kaposvár University, the Hungarian Branch of the WRSA and the Rabbit Production Board. This is the largest and most popular event for rabbit breeders in Hungary. Thirteen papers were presented by senior and young scientists. Topics of the papers covered almost all fields of rabbit research (production, housing and welfare, reproduction, genetics, nutrition. Full papers are available from the organiser (matics.zsolt@ke.hu on request.

  4. An Annotated Bibliography on the Rabbit Novels by John Updike

    OpenAIRE

    2011-01-01

    Se presenta un registro comentado de los principales estudios, libros y artículos sobre la serie narrativa del novelista estadounidense John Updike, conocida bajo el título común de novelas sobre Conejo (Corre, Conejo; El regreso de Conejo; Conejo es rico, etc.).This annotated bibliography includes a brief introduction to the Rabbit novels and comments on the main studies, books and articles on these novels by the U.S. writer John Updike (Rabbit, Run; The Redux of Rabbit; Rabbit Is Rich, etc....

  5. Abstracts of the 24th Hungary conference on rabbit production

    Directory of Open Access Journals (Sweden)

    24TH Conference on rabbit production Kaposvár, Hungary. 30th May, 2012

    2014-09-01

    Full Text Available Some 100 guests took part in the 24th Hungarian Conference on Rabbit Production in Kaposvár, organised by the University of Kaposvár, the Hungarian Branch of the WRSA and the Rabbit Production Board. This is the largest and most popular event for rabbit breeders in Hungary. Seventeen papers were presented, both by senior and young scientists. Topics of the papers covered all fields of rabbit production (production, housing and welfare, reproduction, genetics, nutrition, meat quality and pathology. Full papers are available from the organiser (matics.zsolt@ke.hu on request.

  6. The Gould Belt, the de Vaucouleurs-Dolidze Belt, and the Orion Arm

    OpenAIRE

    Bobylev, V. V.; Bajkova, A. T.

    2014-01-01

    Based on masers with measured trigonometric parallaxes, we have redetermined the spatial orientation parameters of the Local (Orion) arm. Using 23 sources (the Gould Belt objects were excluded), we have found that their spatial distribution can be approximated by a very narrow ellipsoid elongated in the direction L_1=77.1+/-2.9^o whose symmetry plane is inclined to the Galactic plane at an angle of 5.6+/-0.2^o. The longitude of the ascending node of the symmetry plane is l_\\Omega=70+/-3^o. A ...

  7. Forming the Cold Classical Kuiper Belt in a light Disk

    CERN Document Server

    Shannon, Andrew; Lithwick, Yoram

    2015-01-01

    Large Kuiper Belt Objects are conventionally thought to have formed out of a massive planetesimal belt that is a few thousand times its current mass. Such a picture, however, is incompatible with multiple lines of evidence. Here, we present a new model for the conglomeration of Cold Classical Kuiper belt objects, out of a solid belt only a few times its current mass, or a few percent of the solid density in a Minimum Mass Solar Nebula. This is made possible by depositing most of the primordial mass in grains of size centimetre or smaller. These grains collide frequently and maintain a dynamically cold belt out of which large bodies grow efficiently: an order-unity fraction of the solid mass can be converted into large bodies, in contrast to the ~0.1% efficiency in conventional models. Such a light belt may represent the true outer edge of the Solar system, and it may have effectively halted the outward migration of Neptune. In addition to the high efficiency, our model can also produce a mass spectrum that pe...

  8. Orion revisited III. The Orion Belt population

    CERN Document Server

    Kubiak, K; Bouy, H; Sarro, L M; Ascenso, J; Burkert, A; Forbrich, J; Großschedl, J; Hacar, A; Hasenberger, B; Lombardi, M; Meingast, S; Köhler, R; Teixeira, P S

    2016-01-01

    This paper continues our study of the foreground population to the Orion molecular clouds. The goal is to characterize the foreground population north of NGC 1981 and to investigate the star formation history in the large Orion star-forming region. We focus on a region covering about 25 square degrees, centered on the $\\epsilon$ Orionis supergiant (HD 37128, B0\\,Ia) and covering the Orion Belt asterism. We used a combination of optical (SDSS) and near-infrared (2MASS) data, informed by X-ray (\\textit{XMM-Newton}) and mid-infrared (WISE) data, to construct a suite of color-color and color-magnitude diagrams for all available sources. We then applied a new statistical multiband technique to isolate a previously unknown stellar population in this region. We identify a rich and well-defined stellar population in the surveyed region that has about 2\\,000 objects that are mostly M stars. We infer the age for this new population to be at least 5\\, Myr and likely $\\sim10$\\,Myr and estimate a total of about 2\\,500 mem...

  9. Dark nebulae, dark lanes, and dust belts

    CERN Document Server

    Cooke, Antony

    2012-01-01

    As probably the only book of its type, this work is aimed at the observer who wants to spend time with something less conventional than the usual fare. Because we usually see objects in space by means of illumination of one kind or another, it has become routine to see them only in these terms. However, part of almost everything that we see is the defining dimension of dark shading, or even the complete obscuration of entire regions in space. Thus this book is focused on everything dark in space: those dark voids in the stellar fabric that mystified astronomers of old; the dark lanes reported in many star clusters; the magical dust belts or dusty regions that have given so many galaxies their identities; the great swirling 'folds' that we associate with bright nebulae; the small dark feature detectable even in some planetary nebulae; and more. Many observers pay scant attention to dark objects and details. Perhaps they are insufficiently aware of them or of the viewing potential they hold, but also it may be...

  10. Politicians and Epidemics in the Bible Belt

    Directory of Open Access Journals (Sweden)

    Geurt Henk Spruyt

    2016-07-01

    Full Text Available The Netherlands has a high vaccination coverage, but due to a concentration of people objecting to vaccination on religious grounds in the Bible belt, several polio and measles epidemics have occurred there in the past. This article analyses how and in what way Dutch politicians responded to parents refusing to have their children vaccinated. First, during these epidemics Members of Parliament mainly addressed written questions of a highly technical or procedural nature to the responsible State Secretary or Minister of Public Health. Secondly, the government always requested advice from an advisory body with regard to matters of health after an epidemic. These reports were opposed to mandatory vaccination mainly by using practical arguments. However, by advocating the idea of strengthening the position of minors in public healthcare these reports contributed to legislation codifying the rights of minors concerning public health. The most important rationale behind this response is the complexity of the issue of mandatory vaccination, because different human rights are involved. On the one hand, mandatory vaccination infringes the constitutional rights to the freedom of thought, conscience and religion and encroaches on parental autonomy. On the other hand, children have a right to physical integrity. To find a way out of this unsolvable dilemma, the government and its advisory bodies mainly used practical arguments against mandatory vaccination.

  11. Trapped radiation belts of saturn: first look.

    Science.gov (United States)

    Fillius, W; Ip, W H; McIlwain, C E

    1980-01-25

    Pioneer 11 has made the first exploration of the magnetosphere and trapped radiation belts of Saturn. Saturn's magnetosphere is intermediate in size between Earth's and Jupiter's, with trapped particle intensities comparable to Earth's. The outer region of Saturn's magnetosphere contains lower energy radiation and is variable with time; the inner region contains higher energy particles. The pitch angle distributions show a remarkable variety of field-aligned and locally mirroring configurations. The moons and especially the rings of Saturn are effective absorbers of trapped particles; underneath the rings, the trapped radiation is completely absorbed. We confirm the discovery of a new ring, called the F ring, a new division, the Pioneer division, and a moon, called 1979 S 2. The latter has probably been seen from Earth. There may be evidence for more bodies like 1979 S 2, but at this stage the interpretation of the data is ambiguous. Using particle diffusion rates, we estimate that the cross-sectional area of the F ring is > 7 x 10(13) square centimeters and that the opacity is > 10(-5). Cosmic-ray albedo neutron decay should be looked into as a source of energetic particles in the inner magnetosphere of Saturn.

  12. Radiation Belt Electron Dynamics: Modeling Atmospheric Losses

    Science.gov (United States)

    Selesnick, R. S.

    2003-01-01

    The first year of work on this project has been completed. This report provides a summary of the progress made and the plan for the coming year. Also included with this report is a preprint of an article that was accepted for publication in Journal of Geophysical Research and describes in detail most of the results from the first year of effort. The goal for the first year was to develop a radiation belt electron model for fitting to data from the SAMPEX and Polar satellites that would provide an empirical description of the electron losses into the upper atmosphere. This was largely accomplished according to the original plan (with one exception being that, for reasons described below, the inclusion of the loss cone electrons in the model was deferred). The main concerns at the start were to accurately represent the balance between pitch angle diffusion and eastward drift that determines the dominant features of the low altitude data, and then to accurately convert the model into simulated data based on the characteristics of the particular electron detectors. Considerable effort was devoted to achieving these ends. Once the model was providing accurate results it was applied to data sets selected from appropriate periods in 1997, 1998, and 1999. For each interval of -30 to 60 days, the model parameters were calculated daily, thus providing good short and long term temporal resolution, and for a range of radial locations from L = 2.7 to 3.9. .

  13. Trapped radiation belts of Saturn - First look

    Science.gov (United States)

    Fillius, W.; Ip, W. H.; Mcilwain, C. E.

    1980-01-01

    Data on the magnetosphere of Saturn obtained with the trapped radiation detector package on board the Pioneer 11 spacecraft is reported. Radiation belt profiles determined by the trapped radiation detectors on Pioneer 10 and 11 indicate that Saturn's magnetosphere is intermediate in size between those of the earth and Jupiter, with particle intensities similar to those of the earth. The outer region of the Saturn magnetosphere is found to contain particles of lower energy than the outer region, being strongly influenced by the time-varying solar wind. The moons and rings of Saturn are observed to be effective absorbers of trapped particles, confirming the discoveries of the F ring, the Pioneer ring division and the moon 1979 S 2. Particle diffusion rates are used to estimate a cross-sectional area of greater than 7 x 10 to the 13th sq cm and an opacity greater than 0.00001 for the F ring. It is suggested that cosmic-ray albedo neutron decay be studied as a possible source of energetic particles in the inner magnetosphere of Saturn.

  14. Surgical Treatment of Facial Abscesses and Facial Surgery in Pet Rabbits.

    Science.gov (United States)

    Capello, Vittorio

    2016-09-01

    Odontogenic facial abscesses associated with periapical infections and osteomyelitis of the jaw represent an important part of the acquired and progressive dental disease syndrome in pet rabbits. Complications such as retromasseteric and retrobulbar abscesses, extensive osteomyelitis of the mandible, and empyemas of the skull are possible sequelae. Standard and advanced diagnostic imaging should be pursued to make a detailed and proper diagnosis, and plan the most effective surgical treatment. This article reviews the surgical anatomy, the pathophysiology, and the classification of abscesses and empyemas of the mandible, the maxilla, and the skull. It also discusses surgical techniques for facial abscesses.

  15. A bovine herpesvirus 5 recombinant defective in the thymidine kinase (TK gene and a double mutant lacking TK and the glycoprotein E gene are fully attenuated for rabbits

    Directory of Open Access Journals (Sweden)

    S.C. Silva

    2010-02-01

    Full Text Available Bovine herpesvirus 5 (BoHV-5, the agent of herpetic meningoencephalitis in cattle, is an important pathogen of cattle in South America and several efforts have been made to produce safer and more effective vaccines. In the present study, we investigated in rabbits the virulence of three recombinant viruses constructed from a neurovirulent Brazilian BoHV-5 strain (SV507/99. The recombinants are defective in glycoprotein E (BoHV-5gEΔ, thymidine kinase (BoHV-5TKΔ and both proteins (BoHV-5gEΔTKΔ. Rabbits inoculated with the parental virus (N = 8 developed neurological disease and died or were euthanized in extremis between days 7 and 13 post-infection (pi. Infectivity was detected in several areas of their brains. Three of 8 rabbits inoculated with the recombinant BoHV-5gEΔ developed neurological signs between days 10 and 15 pi and were also euthanized. A more restricted virus distribution was detected in the brain of these animals. Rabbits inoculated with the recombinants BoHV-5TKΔ (N = 8 or BoHV-5gEΔTKΔ (N = 8 remained healthy throughout the experiment in spite of variable levels of virus replication in the nose. Dexamethasone (Dx administration to rabbits inoculated with the three recombinants at day 42 pi did not result in viral reactivation, as demonstrated by absence of virus shedding and/or increase in virus neutralizing titers. Nevertheless, viral DNA was detected in the trigeminal ganglia or olfactory bulbs of all animals at day 28 post-Dx, demonstrating they were latently infected. These results show that recombinants BoHV-5TKΔ and BoHV-5gEΔTKΔ are attenuated for rabbits and constitute potential vaccine candidates upon the confirmation of this phenotype in cattle.

  16. Atomic model of rabbit hemorrhagic disease virus by cryo-electron microscopy and crystallography.

    Science.gov (United States)

    Wang, Xue; Xu, Fengting; Liu, Jiasen; Gao, Bingquan; Liu, Yanxin; Zhai, Yujia; Ma, Jun; Zhang, Kai; Baker, Timothy S; Schulten, Klaus; Zheng, Dong; Pang, Hai; Sun, Fei

    2013-01-01

    Rabbit hemorrhagic disease, first described in China in 1984, causes hemorrhagic necrosis of the liver. Its etiological agent, rabbit hemorrhagic disease virus (RHDV), belongs to the Lagovirus genus in the family Caliciviridae. The detailed molecular structure of any lagovirus capsid has yet to be determined. Here, we report a cryo-electron microscopic (cryoEM) reconstruction of wild-type RHDV at 6.5 Å resolution and the crystal structures of the shell (S) and protruding (P) domains of its major capsid protein, VP60, each at 2.0 Å resolution. From these data we built a complete atomic model of the RHDV capsid. VP60 has a conserved S domain and a specific P2 sub-domain that differs from those found in other caliciviruses. As seen in the shell portion of the RHDV cryoEM map, which was resolved to ~5.5 Å, the N-terminal arm domain of VP60 folds back onto its cognate S domain. Sequence alignments of VP60 from six groups of RHDV isolates revealed seven regions of high variation that could be mapped onto the surface of the P2 sub-domain and suggested three putative pockets might be responsible for binding to histo-blood group antigens. A flexible loop in one of these regions was shown to interact with rabbit tissue cells and contains an important epitope for anti-RHDV antibody production. Our study provides a reliable, pseudo-atomic model of a Lagovirus and suggests a new candidate for an efficient vaccine that can be used to protect rabbits from RHDV infection.

  17. An experimental model of mycobacterial infection under corneal flaps

    Directory of Open Access Journals (Sweden)

    C.B.D. Adan

    2004-07-01

    Full Text Available In order to develop a new experimental animal model of infection with Mycobacterium chelonae in keratomileusis, we conducted a double-blind prospective study on 24 adult male New Zealand rabbits. One eye of each rabbit was submitted to automatic lamellar keratotomy with the automatic corneal shaper under general anesthesia. Eyes were immunosuppressed by a single local injection of methyl prednisolone. Twelve animals were inoculated into the keratomileusis interface with 1 µl of 10(6 heat-inactivated bacteria (heat-inactivated inoculum controls and 12 with 1 µl of 10(6 live bacteria. Trimethoprim drops (0.1%, w/v were used as prophylaxis for the surgical procedure every 4 h (50 µl, qid. Animals were examined by 2 observers under a slit lamp on the 1st, 3rd, 5th, 7th, 11th, 16th, and 23rd postoperative days. Slit lamp photographs were taken to document clinical signs. Animals were sacrificed when corneal disease was detected and corneal samples were taken for microbiological analysis. Eleven of 12 experimental rabbits developed corneal disease, and M. chelonae could be isolated from nine rabbits. Eleven of the 12 controls receiving a heat-inactivated inoculum did not develop corneal disease. M. chelonae was not isolated from any of the control rabbits receiving a heat-inactivated inoculum, or from the healthy cornea of control rabbits. Corneal infection by M. chelonae was successfully induced in rabbits submitted to keratomileusis. To our knowledge, this is the first animal model of M. chelonae infection following corneal flaps for refractive surgery to be described in the literature and can be used for the analysis of therapeutic responses.

  18. Market Driving to Develop Rabbit Meat Products in Indonesia

    Directory of Open Access Journals (Sweden)

    Atien Priyanti

    2012-09-01

    Full Text Available Rabbit meat is a nutritional food containing high protein and low cholesterol, fat and sodium. Current research in rabbit production is aimed for developing production strategies to increase the nutritional and economic values of rabbit meat products as functional food. Nowadays, producing rabbit is a popular farming activity in many parts of Indonesia as a small and medium scale operation for food security and cash income. Rabbit farming is to produce meat, skin and hides, fur, organic fertilizers and pet or fancy animals. Consumption of rabbit meat is considered very low, due partly to low meat supply and inavailability of marketing. In some tourist areas, such as Lembang (West Java, Tawangmangu (Central Java, Sarangan and Batu (East Java rabbit meat is a specific food. Attempt to create and drive rabbit markets will simultaneously increase meat production to fulfill the demand and meet economic scale of farming. Hence, this will give significant impact to the farmers’ welfare. Availability of good quality meat, dissemination and diversification of meat products, production efficiency toward competitive price along with its proper marketing strategy will drive consumers’ preferences to consume more rabbit meat. Market driving needs to be created in order to promote rabbit meat products by establishing food outlets. This program has been developed by a farmers group in Magelang, Central Java. During the period of 2006 – 2007 the food outlets had increased to 5 outlets, and in 2012 become 9 outlets. This market driving will also have an impact on changing orientation of rabbit farming from traditional to a small and medium economic scale that will influence the production efficiency.

  19. Evaluation of legislation on seat belt use on rear seats : social persuasion as a new measure to promote seat belt use.

    NARCIS (Netherlands)

    Oei, H.-l.

    1996-01-01

    In the Netherlands, the use of seat belts for front seat passengers was made compulsory in 1975, and on April 1st 1992 the use of rear seat belts, if fitted, became a legal requirement. Since 1968, an annual survey of the presence and use of seat belts on front seats - extended in 1989 to include re

  20. Seat Belts Pay Off. The Use of Economic Incentives and Public Education to Increase Seat Belt Use in a Community. Final Report.

    Science.gov (United States)

    Campbell, B. J.; And Others

    A six-month campaign to increase seat belt use in Chapel Hill and Carrboro, North Carolina centered around the idea of giving out economic incentives for seat belt wearing. The approach was to stop vehicles at random and give all belted vehicle occupants a small prize and a chance for a large cash prize. Precampaign activities involve collecting…

  1. Molecular Dynamics Studies on the Structural Stability of Wild-Type Rabbit Prion Protein: Surface Electrostatic Charge Distributions

    CERN Document Server

    Zhang, Jiapu

    2011-01-01

    Prion diseases cover a large range of neurodegenerative diseases in humans and animals, which are invariably fatal and highly infectious. By now there have not been some effective therapeutic approaches or medications to treat all prion diseases. Fortunately, numerous experimental experiences have showed that rabbits are resistant to infection from prion diseases isolated from other species, and recently the molecular structures of rabbit prion protein and its mutants were released into protein data bank. Prion diseases are "protein structural conformational" diseases. Thus, in order to reveal some secrets of prion diseases, it is amenable to study rabbits by techniques of the molecular structure and its dynamics. Wen et al. (PLoS One 5(10) e13273 (2010), Journal of Biological Chemistry 285(41) 31682-31693 (2010)) reported the surface of NMR RaPrPC(124-228) molecular snapshot has a large land of continuous positive charge distribution, which contributes to the structural stability of rabbit prion protein. Thi...

  2. Surface Ig on rabbit lymphocytes. Rabbit B and T cells are distinct populations

    NARCIS (Netherlands)

    Bast, B J; Catty, D; Manten-Slingerland, R; Jansen, J T; Veldhuis, Dick H.; Roholl, P; Ballieux, R E

    1979-01-01

    Rabbit peripheral blood lymphocytes (PBL) were analyzed by immunofluorescence using anti-T cell conjugates and anti-Fab, anti-a1 allotype, anti-IgM and anti-IgA conjugates. In addition, T cells were demonstrated by rosetting with papain-treated homologous erythrocytes. Control experiments, using aci

  3. The relationship between the plasmapause and outer belt electrons

    Science.gov (United States)

    Goldstein, J.; Baker, D. N.; Blake, J. B.; De Pascuale, S.; Funsten, H. O.; Jaynes, A. N.; Jahn, J.-M.; Kletzing, C. A.; Kurth, W. S.; Li, W.; Reeves, G. D.; Spence, H. E.

    2016-09-01

    We quantify the spatial relationship between the plasmapause and outer belt electrons for a 5 day period, 15-20 January 2013, by comparing locations of relativistic electron flux peaks to the plasmapause. A peak-finding algorithm is applied to 1.8-7.7 MeV relativistic electron flux data. A plasmapause gradient finder is applied to wave-derived electron number densities >10 cm-3. We identify two outer belts. Outer belt 1 is a stable zone of >3 MeV electrons located 1-2 RE inside the plasmapause. Outer belt 2 is a dynamic zone of plasma density. Belt 1 decayed on hiss timescales prior to a disturbance on 17 January and suffered only a modest dropout, perhaps owing to shielding by the plasmasphere. Afterward, the partially depleted belt 1 continued to decay at the initial rate. Belt 2 was emptied out by strong disturbance-time losses but restored within 24 h. For global context we use a plasmapause test particle simulation and derive a new plasmaspheric index Fp, the fraction of a circular drift orbit inside the plasmapause. We find that the locally measured plasmapause is (for this event) a good proxy for the globally integrated opportunity for losses in cold plasma. Our analysis of the 15-20 January 2013 time interval confirms that high-energy electron storage rings can persist for weeks or even months if prolonged quiet conditions prevail. This case study must be followed up by more general study (not limited to a 5 day period).

  4. Biomechanics of 4-point seat belt systems in farside impacts.

    Science.gov (United States)

    Rouhana, Stephen W; Kankanala, Sundeep V; Prasad, Priya; Rupp, Jonathan D; Jeffreys, Thomas A; Schneider, Lawrence W

    2006-11-01

    The biomechanical behavior of a harness style 4-point seat belt system in farside impacts was investigated through dummy and post mortem human subject tests. Specifically, this study was conducted to evaluate the effect of the inboard shoulder belt portion of a 4-point seat belt on the risk of vertebral and soft-tissue neck injuries during simulated farside impacts. Two series of sled tests simulating farside impacts were completed with crash dummies of different sizes, masses and designs to determine the forces and moments on the neck associated with loading of the shoulder belt. The tests were also performed to help determine the appropriate dummy to use in further testing. The BioSID and SID-IIs reasonably simulated the expected kinematics response and appeared to be reasonable dummies to use for further testing. Analysis also showed that dummy injury measures were lower than injury assessment reference values used in development of side impact airbags. Six post-mortem human subjects, three small females and three medium sized males, were tested under conditions similar to those used for the dummy tests. The carotid arteries were pressurized in an attempt to simulate the corresponding neck vascular response of living humans. Post-test autopsies conducted on all test subjects indicated an absence of test-induced arterial or vertebral injuries. Further, comparative analysis of kinematics confirmed the adequacy of the BioSID and SID-IIs in simulating cadaveric response in farside impacts with harness style 4-point belts. A number of issues remain to be solved before the implementation of 4-point seat belts in vehicles, including, among others, the risk of injury to a pregnant woman and her fetus in frontal crashes. The risk of fetal injury in pregnant occupants may be related to the location of the 4-point seat belt's buckle and latch junction at the centerline of the mother's abdomen.

  5. Meningococcal carriage in the African meningitis belt

    Science.gov (United States)

    2013-01-01

    A meningococcal serogroup A polysaccharide/tetanus toxoid conjugate vaccine (PsA-TT) (MenAfriVac#x2122;) is being deployed in countries of the African meningitis belt. Experience with other polysaccharide/protein conjugate vaccines has shown that an important part of their success has been their ability to prevent the acquisition of pharyngeal carriage and hence to stop transmission and induce herd immunity. If PsA-TT is to achieve the goal of preventing epidemics, it must be able to prevent the acquisition of pharyngeal carriage as well as invasive meningococcal disease and whether PsA-TT can prevent pharyngeal carriage needs to be determined. To address this issue, a consortium (the African Meningococcal Carriage (MenAfriCar) consortium) was established in 2009 to investigate the pattern of meningococcal carriage in countries of the African meningitis belt prior to and after the introduction of PsA-TT. This article describes how the consortium was established, its objectives and the standardised field and laboratory methods that were used to achieve these objectives. The experience of the MenAfriCar consortium will help in planning future studies on the epidemiology of meningococcal carriage in countries of the African meningitis belt and elsewhere. Un vaccin conjugué contenant un polysaccharide du sérogroupe A méningococcique et une anatoxine du tétanos (PsA-TT) (MenAfriVac™) est en cours de déploiement dans les pays de la ceinture africaine de la méningite. L’ expérience avec d’ autres vaccins conjugués polysaccharide/protéine a montré qu’ une partie importante de leur succès a été leur capacité à empêcher l’ acquisition du portage pharyngé et donc à arrêter la transmission et à induire une immunité de group. Si PsA-TT doit d’ atteindre l’ objectif de prévenir les épidémies, il devrait être en mesure d’ empêcher l’ acquisition du portage pharyngé ainsi que la méningococcie invasive et le fait que PsA-TT puisse emp

  6. Digital Spectra and Analysis of Altitudinal Belts in Tianshan Mountains,China

    Institute of Scientific and Technical Information of China (English)

    ZHANG Baiping; MO Shenguo; WU Hongzhi; XIAO Fei

    2004-01-01

    Based on the framework of the geo-info spectra of montane altitudinal belts, this paper firstly reviews six classification systems for the spectra of mountain altitudinal belts in China and considers that detailed regional study of altitudinal belts is the key for reaching standardization and systemization of mountain altitudinal belts. Only can this furtheridentify and resolve problems with the study of altitudinal belts. The factors forming the spectra of altitudinal belts are analyzed in the Tianshan Mountains of China, and a digital altitudinal belt system is constructed for the northern flank, Southern flank, the heartland, and Ilivalley in the west. The characteristics of each belt are revealed with a summarization of the pattern of areal differentiation of altitudinal belts.

  7. 23 CFR 1240.12 - Determination of State seat belt use rate for calendar year 1998 and beyond.

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Determination of State seat belt use rate for calendar... FOR USE OF SEAT BELTS-ALLOCATIONS BASED ON SEAT BELT USE RATES Determination of Allocations § 1240.12 Determination of State seat belt use rate for calendar year 1998 and beyond. (a) State seat belt use survey....

  8. Protecting short-term intravascular ear catheters in healthy rabbits.

    Science.gov (United States)

    Sampieri, Francesca; Orchard, Rekha N; Antonopoulos, Aphroditi J; Hamilton, Donald L

    2012-01-20

    Researchers may place a catheter in the ear vessel of a rabbit for a short period of time in order to collect repeated blood samples without extensive restraint of the animal. Maintaining such a catheter in a healthy rabbit can be challenging, as the animal may scratch at the ear, removing the catheter or forming a large hematoma that might impede blood sampling. The authors developed a technique for protecting the indwelling catheter by cutting a section of moleskin to the same shape as the ear and gluing it to the surface of the ear and the catheter. They applied this technique to collect multiple blood samples during 12-h periods from nine rabbits in a pharmacokinetics study. Catheters remained patent in five rabbits for 12 h, in two rabbits for 8 h, in one rabbit for 6 h and in one rabbit for 4 h. This technique allowed for collection of repeated blood samples and prevented the rabbits from interfering with the catheter while allowing them to move freely during the sampling period.

  9. Comparative efficacies of lipid-complexed amphotericin B and liposomal amphotericin B against coccidioidal meningitis in rabbits.

    Science.gov (United States)

    Clemons, Karl V; Capilla, Javier; Sobel, Raymond A; Martinez, Marife; Tong, Ann-Jay; Stevens, David A

    2009-05-01

    In separate previous studies, we have shown that lipid-complexed amphotericin B (Abelcet [ABLC]) and liposomal amphotericin B (AmBisome [AmBi]) are efficacious against coccidioidal meningitis in rabbits. Here, we compared ABLC and AmBi directly in a coccidioidal meningitis model. Male New Zealand White rabbits were infected with 5 x 10(4) Coccidioides posadasii arthroconidia by direct cisternal puncture. Therapy with intravenous ABLC or AmBi at 7.5 or 15 mg/kg of body weight or sterile 5% dextrose water (D5W) began 5 days later. Clinical assessments were done daily; cerebrospinal fluid and blood samples were obtained on day 15 and upon euthanasia. Survivors to day 25 were euthanatized, the numbers of CFU in their tissues were determined, and histology analyses of the brains and spinal cords were done. Controls showed progressive disease, whereas animals treated with either dose of either drug showed few clinical signs of infection. All ABLC- or AmBi-treated rabbits survived, whereas eight of nine D5W-treated rabbits were euthanatized before day 25 (P 0.05), ABLC and AmBi were not significantly different. In this model, intravenous ABLC and AmBi were similarly highly effective, with few clinical signs of infection, 100% survival, and significantly reduced fungal burdens among treated animals. There appeared to be little benefit in using the 15-mg/kg dosage of either formulation. There was no significant advantage of one drug over the other for this indication. Further studies are required to determine the lowest effective doses of these formulations.

  10. Efficacies of ceftobiprole medocaril and comparators in a rabbit model of osteomyelitis due to methicillin-resistant Staphylococcus aureus.

    Science.gov (United States)

    Yin, Li-Yan; Calhoun, Jason H; Thomas, Jacob K; Shapiro, Stuart; Schmitt-Hoffmann, Anne

    2008-05-01

    The pharmacokinetics and distribution into bone tissue of ceftobiprole in uninfected New Zealand White rabbits were determined after subcutaneous administration of the prodrug ceftobiprole medocaril. Serum exposure (maximum concentration of the drug in serum, trough concentration, area under the concentration-time curve) to ceftobiprole at 20 and 80 mg/kg was dose proportional, and there was no accumulation of ceftobiprole following repeated (every 6 h [q6h]) injections of the antibiotic. Ceftobiprole titers in the tibial matrix and marrow were 3.2 +/- 1.3 microg/g and 11.2 +/- 6.5 microg/g, respectively, in uninfected animals treated with 20 mg/kg of the antibiotic and 13.4 +/- 7.3 microg/g and 66.3 +/- 43.2 microg/g, respectively, in uninfected animals treated with 80 mg/kg of the antibiotic. No differences in ceftobiprole titers were observed between right and left tibiae for either bone matrix or marrow. The efficacies of 4 weeks of treatment with ceftobiprole (40 mg/kg administered subcutaneously [s.c.] q6h), vancomycin (30 mg/kg administered s.c. q12h), or linezolid (60 mg/kg administered orally q8h) were compared, using a rabbit model of methicillin-resistant Staphylococcus aureus tibial osteomyelitis. After treatment with ceftobiprole, the bacterial titers in all infected left tibiae from evaluable rabbits were below the level of detection, whereas only 73% of infected left tibiae from vancomycin- or linezolid-treated animals had bacterial titers below the level of detection; the mean titers of ceftobiprole were 3 to 5 times higher in infected left tibiae than in uninfected right tibiae. These results indicate that ceftobiprole provided effective parenteral treatment of osteomyelitis in this rabbit model.

  11. Experimental model of bladder instability in rabbits

    Directory of Open Access Journals (Sweden)

    Balasteghin K.T.

    2003-01-01

    Full Text Available OBJECTIVE: Propose a new experimental model of bladder instability in rabbits after partial bladder obstruction. MATERIALS AND METHODS: Thirty North Folk male rabbits, weighting 1,700 to 2,820 g (mean: 2,162 g were studied. The animals were distributed in 2 experimental groups, formed by 15 rabbits each: Group 1 - clinical control. In this group there was no surgical intervention; Group 2 - bladder outlet obstruction. In this group, after anesthetizing the animal, urethral cannulation with Foley catheter 10F was performed and then an adjustable plastic bracelet was passed around the bladder neck. It was then adjusted in order to not constrict the urethra. The following parameters were studied in M1 - pre-operative period; M2 - 4 weeks post-operatively moments: 1- urine culture; 2- cystometric study; 3- serum creatinine and BUN. RESULTS: Bladder weight was 2.5 times larger in the group with obstruction than in the control group. Cystometric evaluation showed a significant increase in maximal vesical volume in the final moment at Group G2. However, there was no statistically significant difference among the groups studied. There was no statistically significant difference between maximal detrusor pressure and vesical compliance in the different moments or in the studied groups. There was an absence of uninhibited detrusor contractions in all the animals in group 1, and involuntary contractions were detected in 93% of group 2 animals. There was no significant variation in BUN and serum creatinine either among the groups or in the same group. CONCLUSIONS: We observed in the group with obstruction a bladder weight 2.5 higher than normal bladders. We detected involuntary contractions in 93% of the animals in group 2, establishing this experimental model as appropriate to secondary bladder instability and partial bladder outlet obstruction.

  12. International Conference on Immunogenetics of the Rabbit.

    Science.gov (United States)

    1983-12-09

    2.~ .* - ,.. 2"? - - .=--. • L A- 12 Genetics and expression of kappa and lambda light chains in BASILEA rabbits. N. McCartney-Francis, G. 0...the bas mRNA also reveal two distinct light chain RNA specie): kappa with the expected size of 1.15 kb and lambda of 1.04 kb size. Poly(A) RNAs of... kappa light chain * with the b5 (97-108) region determined for the first time in this work suggest that this portion, similar to the 96-1l7 3 region

  13. Strange Curves, Counting Rabbits, & Other Mathematical Explorations

    CERN Document Server

    Ball, Keith

    2011-01-01

    How does mathematics enable us to send pictures from space back to Earth? Where does the bell-shaped curve come from? Why do you need only 23 people in a room for a 50/50 chance of two of them sharing the same birthday? In Strange Curves, Counting Rabbits, and Other Mathematical Explorations, Keith Ball highlights how ideas, mostly from pure math, can answer these questions and many more. Drawing on areas of mathematics from probability theory, number theory, and geometry, he explores a wide range of concepts, some more light-hearted, others central to the development of the field and used dai

  14. Surgical Management of Ear Diseases in Rabbits.

    Science.gov (United States)

    Csomos, Rebecca; Bosscher, Georgia; Mans, Christoph; Hardie, Robert

    2016-01-01

    Otitis externa and media are frequently diagnosed disorders in rabbits and are particularly common in lop-eared breeds because of the specific anatomy of the ear canal. Medical management for otitis externa and media often provides only a temporary improvement in clinical signs. Surgery by means of partial or total ear canal ablation (PECA or TECA) combined with lateral bulla osteotomy (LBO) represents a feasible approach that is well tolerated and provides a good clinical outcome. Short-term complications associated with PECA/TECA-LBO include facial nerve paralysis and vestibular disease.

  15. Preliminary research on myosin light chain kinase in rabbit liver

    Institute of Scientific and Technical Information of China (English)

    Bin Ren; Hua-Qing Zhu; Zhao-Feng Luo; Qing Zhou; Yuan Wang; Yu-Zhen Wang

    2001-01-01

    AIM: To study preliminarily the properties of myosin light chain kinase (MLCK) in rabbit liver. METHODS: The expression of MLCK was detected by reverse transcription-polymerase chain reaction (RT-PCR);the MLCK was obtained from rabbit liver, and its activity was analyzed by γ-32P incorporation technique to detect the phosphorylation of myosin light chain. RESULTS: MLCK was expressed in rabbit liver, and the activity of the enzyme was similar to rabbit smooth muscle MLCK, and calmodulin-dependent. When the concentration was 0.65 mg-L-1, the activity was at the highest level. CONCLUSION: MLCK expressed in rabbit liver may catalyze the phosphorylation of myosin light chain, which may play important roles in the regulation of hepatic cell functions.

  16. GROWTH ANALYSIS IN RABBIT USING GOMPERTZ NON-LINEAR MODEL

    Directory of Open Access Journals (Sweden)

    A. Setiaji

    2014-10-01

    Full Text Available An experiment was conducted to compare the growth curve of rabbit. Three breeds of rabbit,namely Indonesian Local Rabbit (IL, Flamish Giant (FG and Rex (R were used in the study.Individual body weights of each breed was measured from birth to 63 days of age with 3-days interval.Those periodical data were separated into different sex, be then it was averaged to analysis growthpattern. Growth curve parameters were estimated to fit growth data. There was no difference in bodyweight between sexs within breed. Indonesian local rabbit had the lowest body weight. The resultsshowed that growth curve paramaters among three breeds were significantly different (P<0.05 for bothsexes. FG had the highest value of asymptotic mature weight, followed by R and IL. In conclusion,Gompertz model was excellent fit for the growth data in rabbit with a high coefficient determination (R2= 0.999.

  17. 兔脊柱结核模型的构建%Establishment of a rabbit model of spinal tuberculosis with mycobacterium tuberculosis H37Rv

    Institute of Scientific and Technical Information of China (English)

    刘正文; 呼西旦; 付明花; 折胜利; 罗兰; 宋兴华

    2012-01-01

    目的 在兔腰椎椎体钻孔种植人型H37 Rv结核标准菌株构建兔脊柱结核模型.方法 对16只兔腰5椎体上方钻孔,填充明胶海绵,吸附结核菌混悬液0.1 ml(菌量:5g/L)骨蜡封闭钻孔,在术后不同时间段行大体观察、影像学、组织病理学、细菌学等检查.结果 16只大白兔中,其中13只兔腰椎椎体感染结核菌后,局部反应较明显,全身反应较轻,完成实验;3只未感染结核杆菌.在感染的大白兔中,2只出现消瘦、厌食等症状;术后1个月X线阳性4只,术后2个月X线阳性8只、CT阳性12只、MRI阳性13只;感染大白兔的椎体、椎旁软组织切片苏木素-伊红(HE)染色示有脓细胞、类上皮样细胞或坏死灶等形成,对形成的脓肿进行脓液培养显示69.2%结核分枝杆菌生长.建立模型成功率为69.2%.结论 通过在兔脊柱椎体局部进行适当剂量H37 Rv结核标准菌株的种植感染,可构建出与人类脊柱结核病理变化相似的兔脊柱结核.%Objective Drill a hole in the lumbar vertebral of the rabbit and implant human type H37 Rv tuberculosis standard strain in the hole to construct the model of spinal tuberculosis of the rabbit.Methods Drill holes at the top of the 5th anodic lumbar vertebrae in 16 rabbits,and fill the holes with gelatin sponge to adsorb the M.tuberculosis suspension 0.1 ml ( the amount of bacteria:5 g/L).Finally,close the holes with bone wax.After operation,adopt examinations of general observation,Imaging,Histopathology,Bacteriology etc at different time periods.Results 13 rabbits whose lumbar vertebral bodies were infected had obvious partial response and lesser response of whole bodies.3 of the 16 rabbits were not infected by mycobacterium tuberculosis.2 of the infected ones appeared to have weight loss,anorexia and other symptoms.One month after operation,4 rabbits' X-ray findings were positive; 4 months after operation,8 rabbits' X-ray findings were positive,12 rabbits' CT

  18. The Ivisartoq Greenstone Belt, Southwest Greenland: New Investigations

    Science.gov (United States)

    Mader, M. M.; Myers, J. S.; Sylvester, P. J.

    2004-05-01

    The Ivisartoq greenstone belt is situated 40 km south of the Isua greenstone belt, within the Archean gneiss complex of southwest Greenland. The Isua region has been the focus of intense study because it contains some of the oldest known (~ 3.8-3.7 Ga) rocks on Earth. However, relatively little research has been conducted within the Ivisartoq belt. The Ivisartoq greenstone belt is exceptionally well-exposed in three dimensions, and primary features are better preserved here than in any other Archean greenstone belt in Greenland. The Ivisartoq greenstone belt provides a unique opportunity to characterise early-middle Archean mafic-ultramafic magmatism. The Ivisartoq greenstone belt forms a southwest-closing, V-shaped synform and was subjected to amphibolite facies metamorphic conditions. New field mapping has concentrated on the 3 km thick southern limb of the synform, where the most complete section of the tectonostratigraphy is preserved, and where pillow structures with unambiguous way-up indicators are observed. The southern limb has been divided into two units of amphibolites: a lower unit and an upper unit. The upper amphibolite unit is characterized by heterogeneously deformed pillow structures interlayered with ultramafic amphibole schists, meta-gabbro, and with boudins of olivine-bearing ultrabasic rocks. The lower amphibolite unit is more heterogenous and intensely deformed than the upper unit. Layers of quartz-feldspathic rocks with sulfides are abundant in both the upper and lower unit and are highly sheared. A thick (~ 500m) section of quartzo-feldspathic gneisses and schists in the lower unit has been the focus of preliminary geochronological work. Previously, only one U/Pb zircon age (~ 2580 Ma from a "paraschist") has been obtained from the Ivisartoq belt and was reported by Baadsgaard in 1976. Currently, laser ablation microprobe inductively coupled plasma - mass spectrometry (LAM ICP-MS) and isotope dilution thermal ionization mass spectrometry

  19. Formation and Dynamical Evolution of the Asteroid Belt

    Science.gov (United States)

    Bottke, William F.

    2015-08-01

    Asteroids are critical to our desire to unravel the origin of the Solar System because they supply unique, relatively pristine snapshots of the environment in which the Earth formed and evolved. This is due to the fact that, although the asteroids and Earth have followed very different evolutionary pathways, they all formed from the same set of physical processes and share a common ancestry. The asteroid belt presents a particular challenge to understanding terrestrial planet formation because of its small mass. Models of the protoplanetary disk suggest the region between 2-3 AU should contain roughly 3 Earth masses, while less than 0.001 of an Earth mass is actually found there.A long-standing explanation for the asteroid belt's small mass is that it is due to the gravitational influence of Jupiter and Saturn. Some have suggested protoplanets grew there before they were dynamically removed from the asteroid belt by resonances with the gas giants. This left the asteroid belt dynamically excited (which is observed) and heavily depleted in mass. More recently, however, detailed models have shown that this process produces an asteroid belt that is inconsistent with observations.Two recent models propose new ways to match asteroid belt constraints. The first, the so-called ‘Grand Tack’ scenario, uses the results of hydrodynamic simulations to show that Jupiter (and Saturn) migrated both inward and outward across the asteroid belt while interacting with the protoplanetary gas disk. The Grand Tack not only reproduces the mass and mixture of spectral types in the asteroid belt, but it also truncates the planetesimal disk from which the terrestrial planets form, potentially explaining why Mars is less massive than Earth. In a second scenario, planetesimals that form directly from cm- to meter-sized objects, known as “pebbles”, are rapidly converted to 100 to 1000 km asteroid-like object that subsequently grow by accreting even more pebbles. Pebble accretion models

  20. A Physical Model of Electron Radiation Belts of Saturn

    Science.gov (United States)

    Lorenzato, L.; Sicard-Piet, A.; Bourdarie, S.

    2012-04-01

    Radiation belts causes irreversible damages on on-board instruments materials. That's why for two decades, ONERA proposes studies about radiation belts of magnetized planets. First, in the 90's, the development of a physical model, named Salammbô, carried out a model of the radiation belts of the Earth. Then, for few years, analysis of the magnetosphere of Jupiter and in-situ data (Pioneer, Voyager, Galileo) allow to build a physical model of the radiation belts of Jupiter. Enrolling on the Cassini age and thanks to all information collected, this study permits to adapt Salammbô jovian radiation belts model to the case of Saturn environment. Indeed, some physical processes present in the kronian magnetosphere are similar to those present in the magnetosphere of Jupiter (radial diffusion; interaction of energetic electrons with rings, moons, atmosphere; synchrotron emission). However, some physical processes have to be added to the kronian model (compared to the jovian model) because of the particularity of the magnetosphere of Saturn: interaction of energetic electrons with neutral particles from Enceladus, and wave-particle interaction. This last physical process has been studied in details with the analysis of CASSINI/RPWS (Radio and Plasma Waves Science) data. The major importance of the wave particles interaction is now well known in the case of the radiation belts of the Earth but it is important to investigate on its role in the case of Saturn. So, importance of each physical process has been studied and analysis of Cassini MIMI-LEMMS and CAPS data allows to build a model boundary condition (at L = 6). Finally, results of this study lead to a kronian electrons radiation belts model including radial diffusion, interactions of energetic electrons with rings, moons and neutrals particles and wave-particle interaction (interactions of electrons with atmosphere particles and synchrotron emission are too weak to be taken into account in this model). Then, to

  1. Parasite-specific antibody profile in the aqueous humor of rabbits with ocular toxocariasis.

    Science.gov (United States)

    Akiyama, Takashi; Ohta, Nobuo

    2010-06-01

    Human ocular toxocariasis is diagnosed using ophthalmologic and immunologic examinations. Many researchers have suggested that intraocular parasite-specific antibody levels are indicative of ocular toxocariasis, but little is known about the time course of the changes in these levels. We therefore investigated the anti-Toxocara canis antibody profile in the aqueous humor in an animal model of ocular toxocariasis. We intravitreally injected T. canis larvae into the right eye of 4 rabbits; 2 rabbits were orally administered T. canis eggs. We collected serum, aqueous humor, and tear samples weekly and determined the serum and aqueous humor levels of anti-T. canis immunoglobulin (Ig)G, IgA, IgM, and IgE antibodies and the tear IgG antibody level by enzyme-linked immunosorbent assay (ELISA). The severity of vitreous opacity and the aqueous humor IgG levels (measured using optical density [OD]) changed concordantly in the larvae-injected eyes; the OD exceeded 0.1 from 2-4 weeks after infection and remained elevated during active intraocular inflammation. However, the aqueous humor IgG levels were also elevated in 6 out of 8 eyes without intraocular larvae in both groups, and were low in 1 eye with live intravitreal larvae. In contrast, the serum IgG and IgM levels and the tear IgG levels increased in all rabbits, regardless of the presence of intraocular inflammation. Vitreous opacity occurred in all intravitreally infected eyes, but significant histopathological evidence of retinal damage was not detected. Thus, besides the presence of intraocular larvae, some other factors in the host may be required for the development of retinal lesions.

  2. Evolution of the Bhandara-Balaghat granulite belt along the southern margin of the Sausar Mobile Belt of central India

    Indian Academy of Sciences (India)

    H M Ramachandra; Abhinaba Roy

    2001-12-01

    The Bhandara-Balaghat granulite (BBG) belt occurs as a 190 km long, detached narrow, linear, NE-SW to ENE-WSW trending belt that is in tectonic contact on its northern margin with the Sausar Group of rocks and is bordered by the Sakoli fold belt in the south. The Bhandara part of the BBG belt is quite restricted, comprising a medium to coarse grained two-pyroxene granulite body that is of gabbroic composition and preserves relic igneous fabric. The main part of the belt in Arjuni-Balaghat section includes metasedimentary (quartzite, BIF, Al- and Mg-Al metapelites) and metaigneous (metaultramafic, amphibolite and two-pyroxene granulite) protoliths interbanded with charnockite and charnockitic gneiss. These rocks, occurring as small bands and enclaves within migmatitic and granitic gneisses, show polyphase deformation and metamorphism. Geochemically, basic compositions show tholeiitic trend without Fe-enrichment, non-komatitic nature, continental affinity and show evolved nature. Mineral parageneses and reaction textures in different rock compositions indicate early prograde, dehydration melt forming reactions followed by orthopyroxene stability with or without melt. Coronitic and symplectitic garnets have formed over earlier minerals indicating onset of retrograde IBC path. Evidences for high temperature ductile shearing are preserved at places. Retrogressive hydration events clearly post-date the above paths. The present study has shown that the BBG belt may form a part of the Bastar Craton and does not represent exhumed oceanic crust of the Bundelkhand Craton. It is further shown that rocks of the BBG belt have undergone an earlier high-grade granulite metamorphism at 2672 ± 54 Ma (Sm-Nd age) and a post-peak granulite metamorphism at 1416 ± 59 Ma (Sm-Nd age, 1380 ± 28 Ma Rb-Sr age). These events were followed by deposition of the Sausar supracrustals and Neoproterozoic Sausar orogeny between 973 ± 63 Ma and 800 ± 16 Ma (Rb-Sr ages).

  3. Out-of-sequence thrusting in polycyclic thrust belts: An example from the Mesozoic Yanshan belt, North China Craton

    Science.gov (United States)

    Li, Chengming; Zhang, Changhou; Cope, Tim D.; Lin, Yi

    2016-09-01

    The EW trending Yanshan belt, an intraplate fold-thrust belt located in the northern North China Craton that has experienced several episodes of deformation widely separated in time, is characterized by out-of-sequence thrusts. According to detailed mapping in the central Yanshan belt, five geometric and stratigraphic criteria used to aid in determining whether a thrust has an out-of-sequence geometry or not can be recognized. They are (1) unconformable relationships, (2) inclination of fault surfaces, (3) irregular changes in apparent offset along strike, (4) short fault length relative to apparent offset, and (5) in-sequence geometry. With the help of these criteria, two generations of out-of-sequence thrusts that postdate the original in-sequence thrusting in the central Yanshan belt are recognized. The ancestral southward verging fold-and-thrust belt that formed prior to 180 Ma was deformed and cut by two younger generations of faults that are probably more deeply rooted and are constrained to between 172-165 Ma and 152-135 Ma. A series of thrusts with opposite vergence formed during the last period, resulting in abundant abnormal field relationships such as younger-on-older thrust relations, fold truncation, and cutting down-section. The nature and occurrence of faults in the Yanshan belt implies that superimposed deformation, a common feature in polycyclic orogenic belts, is a mechanism for the generation of out-of-sequence thrusting. This adds to mechanisms already described in the literature, such as maintaining constant critical taper at an orogenic scale, inhibition of the deformation front, and lateral changes in the nature of the décollement horizons.

  4. Flat belt continuously variable high speed drive. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Kumm, E.L.

    1992-02-01

    A study was undertaken at Kumm Industries funded by DOE in the NBS/DOE Energy-Related Inventions Program starting in August 1990 to design, construct and test a novel very high speed flat belt drive. The test arrangement as shown in Figure 1 consists of a multiple belt-pulley configuration that transmits power from a low speed (2000--4000 RPM) input to a small pulley ``turbine`` (27,000 to 55,000 RPM) and then to the low speed output variable radius pulley (2000--5000 RPM) via a special self-active tensioner. Transmitting 25 HP to and from the ``turbine`` corresponds to obtaining 50 HP in one direction only in a possible turbo compounded engine application. The high speed of the ``turbine`` belts, i.e. 100 meters/sec. at 55,000 RPM, while transferring substantial power is a new much higher operating regime for belts. The study showed that the available belts gave overall test rig efficiencies somewhat above 80% for the higher speeds (50,000 RPM) and higher powers (corresponding to above 90% in the turbocompound application) and a significantly better efficiencies at slightly lower speeds. The tests revealed a number of improved approaches in the design of such high speed drives. It appears that there is considerable possibility for further improvement and application of such equipment.

  5. SEAT BELT USE AMONG CAR USERS IN MALAYSIA

    Directory of Open Access Journals (Sweden)

    S. KULANTHAYAN

    2004-01-01

    Full Text Available The car is the second (40% most common mode of transportation in Malaysia. In terms of fatal road accidents, car drivers constitute about 9.0% and passengers 13.6% of fatalities. The major cause of car occupants' fatality in such accidents is head injuries, which consist of more than half (56.4% of the fatalities. Thus restraining the head and body, the initial position is the most important injury control strategy for car users. The use of seat belts was deemed one of the most effective ways to reduce road accident fatalities in Malaysia and consequently the mandatory seat belt law was enforced in the early seventies. Therefore, a study on factors influencing the compliance behaviour of seat belt use among cars is needed as to date no such research has been undertaken in Malaysia. A questionnaire study was carried out in Selangor, Malaysia on the compliance behaviour of car occupants in relation to seat belt use. A total of 237 respondents were interviewed and the data analysed using logistic regression method. Six variables were found to be significant at 5 percent level (p<0.05: seating position, location of travel, education level, speeding, night-time driving and enforcement. Compliance with the seat belt law was higher among drivers, educated car users, in the presence of enforcement activities, travelling in city-center areas and car users with a positive attitude towards the risks of speeding and night driving.

  6. Reliability models of belt drive systems under slipping failure mode

    Directory of Open Access Journals (Sweden)

    Peng Gao

    2017-01-01

    Full Text Available Conventional reliability assessment and reliability-based optimal design of belt drive are based on the stress–strength interference model. However, the stress–strength interference model is essentially a static model, and the sensitivity analysis of belt drive reliability with respect to design parameters needs further investigations. In this article, time-dependent factors that contribute the dynamic characteristics of reliability are pointed out. Moreover, dynamic reliability models and failure rate models of belt drive systems under the failure mode of slipping are developed. Furthermore, dynamic sensitivity models of belt drive reliability based on the proposed dynamic reliability models are proposed. In addition, numerical examples are given to illustrate the proposed models and analyze the influences of design parameters on dynamic characteristics of reliability, failure rate, and sensitivity functions. The results show that the statistical properties of design parameters have different influences on reliability and failure rate of belt drive in cases of different values of design parameters and different operational durations.

  7. High rabbit abundance proves detrimental to the population growth rate in European rabbit (Oryctolagus cuniculus L. extensive breeding enclosures

    Directory of Open Access Journals (Sweden)

    L. Ruiz-Aizpurua

    2014-09-01

    Full Text Available The European rabbit (Oryctolagus cuniculus L. is a key prey species in Mediterranean ecosystems that has declined in its natural ranges as a result of diseases and loss of habitat. This situation has led to the production of wild rabbits in enclosures in which they can acclimate and breed. The efficiency of these enclosures as extensive breeding systems is defined by their population growth rate (PGR. The aim of this study is to analyse the effect of rabbit abundance on the PGR. This has been done by creating general linear models to explain autumn and spring PGR with the use of rabbit abundance estimates, enclosure size, aerial predation and previous PGR as possible explanatory variables. Rabbit abundance and enclosure size negatively affected the autumn PGR, while only rabbit abundance affected the spring PGR in the best-fit models. It is suggested that maintaining rabbit densities at fewer than 30 rabbits per hectare might help to optimise the efficiency inside enclosures.

  8. Research on Dynamic Tension for Belt Conveyor with Constant Force Automatic Take-Up Assembly

    Institute of Scientific and Technical Information of China (English)

    MENG Guo-ying; CHEN Jing-li; LI Yu-jin

    2003-01-01

    The article Provides a dynamic model for belt conveyor. Based on the drive-force of conveyor, take-up tension of take-up assembly, gravity of conveyor belt and material, and friction between belt and idlers, it gives a viscoelastic dynamic equation for conveyor belt. It presents a calculation method of analytic solution to both viscoelastic dynamic equation and geometric dynamic equation when automatic take-up assembly is applied to belt conveyor. The article also makes a study of design method of limiting and eliminating the conveyor belt's elastic vibration.

  9. Spectrophotometry of Kuiper Belt Objects and Centaurs

    Science.gov (United States)

    Lederer, S. M.; Vilas, F.

    2002-09-01

    We present an ongoing study of Kuiper Belt Objects (KBOs) and Centaurs. We acquired broadband (UBVRI) and medium band photometry with the 4m Mayall telescope at Kitt Peak and the 1.8m Perkins telescope at Lowell Observatory. We present broadband colors of these objects and discuss the results of our search for absorption bands in the visible spectral region. Vilas (Icarus, 111) demonstrated that the existence of the 0.7um absorption feature in asteroids with solar-like colors was strongly correlated with the presence of the 3.0um water of hydration feature. The broad 0.7um absorption band is attributed to a charge-transfer in Fe-bearing hydrated silicates (phyllosilicates), which are a product of aqueous alteration. For aqueous alteration to take place, one must have water ice present in an object, and an energy source to heat the water ice to a liquid water phase. Water ice has already been discovered in some Centaurs (Luu et al. ApJ, 531; Brown AJ, 119), and Durda and Stern (Icarus, 145) estimate that KBOs experience collisional processing regularly throughout their lifetimes. The estimated impact energies are high enough to induce aqueous alteration. We undertook this study to search for evidence of the 0.7um feature in KBOs and Centaurs. We employed medium band Windhorst filters, located at 0.527, 0.666, 0.705, 0.755 and 0.848 um in conjunction with the Mosaic CCD to search for this absorption band, which extends from 0.57-0.83um. Initial analysis suggests that an absorption feature exists near 0.7um in the greyer objects but not the redder objects, following the correlations observed in asteroids. These data are consistent with the absorption band detected near 0.7um by de Bergh et al. (ACM 2002, Berlin) in visible spectra of 2000 EB173 and 2000 GN171. However, further analysis is required to confirm whether the absorption we see is due to phyllosilicates or another source. We will present the results from this analysis. This research was supported through the

  10. Increased virulence of rabbit haemorrhagic disease virus associated with genetic resistance in wild Australian rabbits (Oryctolagus cuniculus).

    Science.gov (United States)

    Elsworth, Peter; Cooke, Brian D; Kovaliski, John; Sinclair, Ronald; Holmes, Edward C; Strive, Tanja

    2014-09-01

    The release of myxoma virus (MYXV) and Rabbit Haemorrhagic Disease Virus (RHDV) in Australia with the aim of controlling overabundant rabbits has provided a unique opportunity to study the initial spread and establishment of emerging pathogens, as well as their co-evolution with their mammalian hosts. In contrast to MYXV, which attenuated shortly after its introduction, rapid attenuation of RHDV has not been observed. By studying the change in virulence of recent field isolates at a single field site we show, for the first time, that RHDV virulence has increased through time, likely because of selection to overcome developing genetic resistance in Australian wild rabbits. High virulence also appears to be favoured as rabbit carcasses, rather than diseased animals, are the likely source of mechanical insect transmission. These findings not only help elucidate the co-evolutionary interaction between rabbits and RHDV, but reveal some of the key factors shaping virulence evolution.

  11. Belt(s) of debris resolved around the Sco-Cen star HIP 67497

    Science.gov (United States)

    Bonnefoy, M.; Milli, J.; Ménard, F.; Vigan, A.; Lagrange, A.-M.; Delorme, P.; Boccaletti, A.; Lazzoni, C.; Galicher, R.; Desidera, S.; Chauvin, G.; Augereau, J. C.; Mouillet, D.; Pinte, C.; van der Plas, G.; Gratton, R.; Beust, H.; Beuzit, J. L.

    2017-01-01

    Aims: In 2015, we initiated a survey of Scorpius-Centaurus A-F stars that are predicted to host warm-inner and cold-outer belts of debris similar to the case of the system HR 8799. The survey aims to resolve the disks and detect planets responsible for the disk morphology. In this paper, we study the F-type star HIP 67497 and present a first-order modelization of the disk in order to derive its main properties. Methods: We used the near-infrared integral field spectrograph (IFS) and dual-band imager IRDIS of VLT/SPHERE to obtain angular-differential imaging observations of the circumstellar environnement of HIP 67497. We removed the stellar halo with PCA and TLOCI algorithms. The disk emission was modeled with the GRaTeR code. Results: We resolve a ring-like structure that extends up to 450 mas ( 50 au) from the star in the IRDIS and IFS data. It is best reproduced by models of a non-eccentric ring with an inclination of 80 ± 1°, a position angle of -93 ± 1°, and a semi-major axis of 59 ± 3 au. We also detect an additional, but fainter, arc-like structure with a larger extension (0.65 arcsec) South of the ring that we model as a second belt of debris at 130 au. We detect ten candidate companions at separations ≥1''. We estimate the mass of putative perturbers responsible for the disk morphology and compare this to our detection limits. Additional data are needed to find those perturbers, and to relate our images to large-scale structures seen with HST/STIS. Based on observations made with ESO Telescopes at the Paranal Observatory under programme ID 097.C-0060(A)This work is based on data products produced at the SPHERE Data Center hosted at OSUG/IPAG, Grenoble.

  12. Metallothionein in rabbit kidneys preserved for transplantation

    Energy Technology Data Exchange (ETDEWEB)

    Elinder, C.G.; Lundgren, G.; Nordberg, M.; Palm, B.; Piscator, M.

    1984-03-01

    Thirteen rabbits were given cadmium injections to achieve cadmium concentrations in kidney cortex ranging from 0.05 to 1 mmole Cd/kg wet weight. Another four animals served as controls. One kidney from each animal was frozen directly to -70/sup 0/C whereas the other kidney was kept for 24 hr at +4/sup 0/C in a preservative (Sachs' solution) to simulate conditions for preservation of human donor kidneys before transplantation. Protein binding of cadmium, zinc and copper in kidney homogenates and the concentration of metallothionein (MT) were measured in the kidney that was frozen directly and in the kidney that had been preserved. No gross differences in either the protein binding of cadmium, zinc and copper or in the MT content were seen between the directly frozen and preserved kidney from the same animal. This indicates that MT is not rapidly broken down in rabbit kidneys which have been preserved similarly to human donor kidneys for 24 hr in a standard preservative solution prior to a transplantation. 27 references, 3 figures, 1 table.

  13. Metallothionein in rabbit kidneys preserved for transplantation.

    Science.gov (United States)

    Elinder, C G; Lundgren, G; Nordberg, M; Palm, B; Piscator, M

    1984-03-01

    Thirteen rabbits were given repeated cadmium injections to achieve cadmium concentrations in kidney cortex ranging from 0.05 to 1 mmole Cd/kg wet weight. Another four animals served as controls. One kidney from each animal was frozen directly to -70 degrees C whereas the other kidney was kept for 24 hr at +4 degrees C in a preservative (Sachs' solution) to simulate conditions for preservation of human donor kidneys before transplantation. Protein binding of cadmium, zinc and copper in kidney homogenates and the concentration of metallothionein (MT) were measured in the kidney that was frozen directly and in the kidney that had been preserved. No gross differences in either the protein binding of cadmium, zinc and copper or in the MT content were seen between the directly frozen and preserved kidneys from the same animal. This indicates that MT is not rapidly broken down in rabbit kidneys which have been preserved similarly to human donor kidneys for 24 hr in a standard preservative solution prior to a transplantation.

  14. Amiodarone Induced Morphological Changes in Rabbit Pneumocytes

    Directory of Open Access Journals (Sweden)

    Fereshteh Mehraein

    2009-01-01

    Full Text Available Objective: Amiodarone as an iodinated benzofuran derivative is a potent antiarrhythmicagent currently used for the treatment of ventricular arrhythmias. Pulmonary toxicityis one of the complications of Amiodarone therapy. The aim of this study was todetermine the toxicity of Amiodarone for pneumocytes.Materials and Methods: 14 male white New Zealand rabbits were divided in a controlgroup and an experimental group. The experimental group was subjected to intraperitoneal injection with a single daily dose of 80 mg/kg Amiodarone for two weeks.The control group received only normal saline. At the end of the injection period, thetwo groups were anesthetized and perfused with Karnovsky fixative. The lung tissuewas removed and fixed, then prepared for light and electron microscope studies.Morphometric studies were made on sections to find nucleus profile dimensions.Results: Light microscope observation showed acute changes in the alveolus includingcongestion of alveolar capillaries and infiltration of red blood cells (RBCs intothe lumen of the alveoli. Electron microscope study of lung tissue revealed abnormalinclusion bodies within type ΙΙ & Ι pneumocytes. The micrographs also showedthe presence of vacuoles in 5% of the type ΙΙ pneumocytes. Morphometric studiesshowed that the nucleus of the cells in the experimental group were smaller than inthe control group (p<0.01.Conclusion: These results indicate that Amiodarone administration can cause damageto pnuemocytes and the alveolus of rabbit lung, so the effectiveness of Amiodaronein long term treatment of heart failure patients is limited because of the developmentof lung toxicity.

  15. Complexation of arsenic species in rabbit erythrocytes.

    Science.gov (United States)

    Delnomdedieu, M; Basti, M M; Styblo, M; Otvos, J D; Thomas, D J

    1994-01-01

    The binding of arsenite, As(III), and arsenate, As(V), by molecules in the intracellular compartment of rabbit erythrocytes has been studied by 1H- and 31P-NMR spectroscopy, uptake of 73As, and ultrafiltration experiments. For intact erythrocytes to which 0.1-0.4 mM arsenite was added, direct evidence was obtained for entry of 76% within 1/2 h and subsequent binding of As(III) by intracellular glutathione and induced changes in the hemoglobin structure (NMR), likely due to binding of As(III). These results were compared with the effect of addition of As(V) on intact erythrocytes and revealed that a smaller amount of As(V) (approximately 25%) enters the cells; the main fraction of As(V) enters the phosphate pathway, depletes ATP, and increases Pi. In contrast, As(III) did not affect the ATP level. Both 1H- and 31P-NMR data indicated striking differences between As(III) and As(V) behavior when incubated with rabbit erythrocytes. These differences were confirmed by 73As uptake and binding experiments. meso-2,3-Dimercaptosuccinic acid (DMSA), a dithiol ligand, released glutathione from its arsenite complexes in erythrocytes.

  16. Residual stress distribution in rabbit limb bones.

    Science.gov (United States)

    Yamada, Satoshi; Tadano, Shigeru; Fujisaki, Kazuhiro

    2011-04-29

    The presence of the residual stresses in bone tissue has been noted and the authors have reported that there are residual stresses in bone tissue. The aim of our study is to measure the residual stress distribution in the cortical bone of the extremities of vertebrates and to describe the relationships with the osteon population density. The study used the rabbit limb bones (femur, tibia/fibula, humerus, and radius/ulna) and measured the residual stresses in the bone axial direction at anterior and posterior positions on the cortical surface. The osteons at the sections at the measurement positions were observed by microscopy. As a result, the average stresses at the hindlimb bones and the forelimb bones were 210 and 149 MPa, respectively. In the femur, humerus, and radius/ulna, the residual stresses at the anterior position were larger than those at the posterior position, while in the tibia, the stress at the posterior position was larger than that at the anterior position. Further, in the femur and humerus, the osteon population densities in the anterior positions were larger than those in the posterior positions. In the tibia, the osteon population density in the posterior position was larger than that in the anterior position. Therefore, tensile residual stresses were observed at every measurement position in the rabbit limb bones and the value of residual stress correlated with the osteon population density (r=0.55, P<0.01).

  17. Lipidome of atherosclerotic plaques from hypercholesterolemic rabbits.

    Science.gov (United States)

    Bojic, Lazar A; McLaren, David G; Shah, Vinit; Previs, Stephen F; Johns, Douglas G; Castro-Perez, Jose M

    2014-12-15

    The cellular, macromolecular and neutral lipid composition of the atherosclerotic plaque has been extensively characterized. However, a comprehensive lipidomic analysis of the major lipid classes within atherosclerotic lesions has not been reported. The objective of this study was to produce a detailed framework of the lipids that comprise the atherosclerotic lesion of a widely used pre-clinical model of plaque progression. Male New Zealand White rabbits were administered regular chow supplemented with 0.5% cholesterol (HC) for 12 weeks to induce hypercholesterolemia and atherosclerosis. Our lipidomic analyses of plaques isolated from rabbits fed the HC diet, using ultra-performance liquid chromatography (UPLC) and high-resolution mass spectrometry, detected most of the major lipid classes including: Cholesteryl esters, triacylglycerols, phosphatidylcholines, sphingomyelins, diacylglycerols, fatty acids, phosphatidylserines, lysophosphatidylcholines, ceramides, phosphatidylglycerols, phosphatidylinositols and phosphatidylethanolamines. Given that cholesteryl esters, triacylglycerols and phosphatidylcholines comprise greater than 75% of total plasma lipids, we directed particular attention towards the qualitative and quantitative assessment of the fatty acid composition of these lipids. We additionally found that sphingomyelins were relatively abundant lipid class within lesions, and compared the abundance of sphingomyelins to their precursor phosphatidylcholines. The studies presented here are the first approach to a comprehensive characterization of the atherosclerotic plaque lipidome.

  18. Lipidome of Atherosclerotic Plaques from Hypercholesterolemic Rabbits

    Directory of Open Access Journals (Sweden)

    Lazar A. Bojic

    2014-12-01

    Full Text Available The cellular, macromolecular and neutral lipid composition of the atherosclerotic plaque has been extensively characterized. However, a comprehensive lipidomic analysis of the major lipid classes within atherosclerotic lesions has not been reported. The objective of this study was to produce a detailed framework of the lipids that comprise the atherosclerotic lesion of a widely used pre-clinical model of plaque progression. Male New Zealand White rabbits were administered regular chow supplemented with 0.5% cholesterol (HC for 12 weeks to induce hypercholesterolemia and atherosclerosis. Our lipidomic analyses of plaques isolated from rabbits fed the HC diet, using ultra-performance liquid chromatography (UPLC and high-resolution mass spectrometry, detected most of the major lipid classes including: Cholesteryl esters, triacylglycerols, phosphatidylcholines, sphingomyelins, diacylglycerols, fatty acids, phosphatidylserines, lysophosphatidylcholines, ceramides, phosphatidylglycerols, phosphatidylinositols and phosphatidylethanolamines. Given that cholesteryl esters, triacylglycerols and phosphatidylcholines comprise greater than 75% of total plasma lipids, we directed particular attention towards the qualitative and quantitative assessment of the fatty acid composition of these lipids. We additionally found that sphingomyelins were relatively abundant lipid class within lesions, and compared the abundance of sphingomyelins to their precursor phosphatidylcholines. The studies presented here are the first approach to a comprehensive characterization of the atherosclerotic plaque lipidome.

  19. Heart rate variability in isolated rabbit hearts.

    Science.gov (United States)

    Frey, B; Heger, G; Mayer, C; Kiegler, B; Stöhr, H; Steurer, G

    1996-11-01

    The presence of heart rate variability (HRV) in patients with cardiac denervation after heart transplantation raised our interest in HRV of isolated, denervated hearts. Hearts from seven adult white ELCO rabbits were transferred to a perfusion apparatus. All hearts were perfused in the working mode and in the Langendorff mode for 20 minutes each. HRV was analyzed in the frequency domain. A computer simulated test ECG at a constant rate of 2 Hz was used for error estimation of the system. In the isolated, denervated heart, HRV was of random, broadband fluctuations, different from the well-characterized oscillations at specific frequencies in intact animals. Mean NN was 423 +/- 51 ms in the Langendorff mode, 406 +/- 33 ms in the working heart mode, and 500 ms in the test ECG. Total power was 663 +/- 207 ms2, 817 +/- 318 ms2, and 3.7 ms2, respectively. There was no significant difference in any measure of HRV between Langendorff and working heart modes. The data provide evidence for the presence of HRV in isolated, denervated rabbit hearts. Left atrial and ventricular filling, i.e., the working heart mode, did not alter HRV, indicating that left atrial or ventricular stretch did not influence the sinus nodal discharge rate.

  20. Effect of freezing on rabbit cultured chondrocytes

    Directory of Open Access Journals (Sweden)

    R.R Filgueiras

    2011-02-01

    Full Text Available This work evaluated the effect of freezing on chondrocytes maintained in culture, aiming the establishment of a cell bank for future application as heterologous implant. Chondrocytes extracted from joint cartilage of nine healthy New Zealand White rabbits were cultivated and frozen with the cryoprotector 5% dimethylsulfoxide for six months. Phenotypic and scanning electron microscopy analyses were carried out to identify morphological and functional differences between fresh and thawed cells. After enzymatic digestion, a total of 4.8x10(5cells per rabbit were obtained. Fresh chondrocytes showed a high mitotic rate and abundant matrix was present up to 60 days of culture. Loss of phenotypic stability was notable in the thawed chondrocytes, with a low labeling of proteoglycans and weak immunostaining of type II collagen. The present study showed important loss of chondrocyte viability under the freezing conditions. For future in vivo studies of heterologous implant, these results suggests that a high number of cells should be implanted in the host site in order to achieve an adequate number of viable cells. Furthermore, the chondrocytes should be implanted after two weeks of culture, when the highest viability rate is found

  1. Analysis of gene expression in rabbit muscle

    Directory of Open Access Journals (Sweden)

    Alena Gálová

    2014-02-01

    Full Text Available Increasing consumer knowledge of the link between diet and health has raised the demand for high quality food. Meat and meat products may be considered as irreplaceable in human nutrition. Breeding livestock to higher content of lean meat and the use of modern hybrids entails problems with the quality of meat. Analysing of livestock genomes could get us a great deal of important information, which may significantly affect the improvement process. Domestic animals are invaluable resources for study of the molecular architecture of complex traits. Although the mapping of quantitative trait loci (QTL responsible for economically important traits in domestic animals has achieved remarkable results in recent decades, not all of the genetic variation in the complex traits has been captured because of the low density of markers used in QTL mapping studies. The genome wide association study (GWAS, which utilizes high-density single-nucleotide polymorphism (SNP, provides a new way to tackle this issue. New technologies now allow producing microarrays containing thousands of hybridization probes on a single membrane or other solid support. We used microarray analysis to study gene expression in rabbit muscle during different developmental age stages. The outputs from GeneSpring GX sotware are presented in this work. After the evaluation of gene expression in rabbits, will be selected genes of interest in relation to meat quality parameters and will be further analyzed by the available methods of molecular biology and genetics.

  2. Innervation of the rabbit cardiac ventricles.

    Science.gov (United States)

    Pauziene, Neringa; Alaburda, Paulius; Rysevaite-Kyguoliene, Kristina; Pauza, Audrys G; Inokaitis, Hermanas; Masaityte, Aiste; Rudokaite, Gabriele; Saburkina, Inga; Plisiene, Jurgita; Pauza, Dainius H

    2016-01-01

    The rabbit is widely used in experimental cardiac physiology, but the neuroanatomy of the rabbit heart remains insufficiently examined. This study aimed to ascertain the architecture of the intrinsic nerve plexus in the walls and septum of rabbit cardiac ventricles. In 51 rabbit hearts, a combined approach involving: (i) histochemical acetylcholinesterase staining of intrinsic neural structures in total cardiac ventricles; (ii) immunofluorescent labelling of intrinsic nerves, nerve fibres (NFs) and neuronal somata (NS); and (iii) transmission electron microscopy of intrinsic ventricular nerves and NFs was used. Mediastinal nerves access the ventral and lateral surfaces of both ventricles at a restricted site between the root of the ascending aorta and the pulmonary trunk. The dorsal surface of both ventricles is supplied by several epicardial nerves extending from the left dorsal ganglionated nerve subplexus on the dorsal left atrium. Ventral accessing nerves are thicker and more numerous than dorsal nerves. Intrinsic ventricular NS are rare on the conus arteriosus and the root of the pulmonary trunk. The number of ventricular NS ranged from 11 to 220 per heart. Four chemical phenotypes of NS within ventricular ganglia were identified, i.e. ganglionic cells positive for choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), and biphenotypic, i.e. positive for both ChAT/nNOS and for ChAT/tyrosine hydroxylase. Clusters of small intensely fluorescent cells are distributed within or close to ganglia on the root of the pulmonary trunk, but not on the conus arteriosus. The largest and most numerous intrinsic nerves proceed within the epicardium. Scarce nerves were found near myocardial blood vessels, but the myocardium contained only a scarce meshwork of NFs. In the endocardium, large numbers of thin nerves and NFs proceed along the bundle of His and both its branches up to the apex of the ventricles. The endocardial meshwork of fine NFs was

  3. Jovian Early Bombardment: planetesimal erosion in the inner asteroid belt

    CERN Document Server

    Turrini, Diego; Magni, Gianfranco

    2012-01-01

    The asteroid belt is an open window on the history of the Solar System, as it preserves records of both its formation process and its secular evolution. The progenitors of the present-day asteroids formed in the Solar Nebula almost contemporary to the giant planets. The actual process producing the first generation of asteroids is uncertain, strongly depending on the physical characteristics of the Solar Nebula, and the different scenarios produce very diverse initial size-frequency distributions. In this work we investigate the implications of the formation of Jupiter, plausibly the first giant planet to form, on the evolution of the primordial asteroid belt. The formation of Jupiter triggered a short but intense period of primordial bombardment, previously unaccounted for, which caused an early phase of enhanced collisional evolution in the asteroid belt. Our results indicate that this Jovian Early Bombardment caused the erosion or the disruption of bodies smaller than a threshold size, which strongly depen...

  4. Regional Fault Systems of Qaidam Basin and Adjacent Orogenic Belts

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The purpose of this paper is to analyze the regional fault systems of Qaidam basin and adjacent orogenic belts. Field investigation and seismic interpretation indicate that five regional fault systems occurred in the Qaidam and adjacent mountain belts, controlling the development and evolution of the Qaidam basin. These fault systems are: (1)north Qaidam-Qilian Mountain fault system; (2) south Qaidam-East Kunlun Mountain fault system; (3)Altun strike-slip fault system; (4)Elashan strike-slip fault system, and (5) Gansen-Xiaochaidan fault system. It is indicated that the fault systems controlled the orientation of the Qaidam basin, the formation and distribution of secondary faults within the basin,the migration of depocenters and the distribution of hydrocarbon accumulation belt.

  5. Gravity inferred subsurface structure of Gadwal Schist belt, Andhra Pradesh

    Indian Academy of Sciences (India)

    G Ramadass; I B Ramaprasada Rao; N Srinivasulu

    2001-03-01

    Detailed gravity data collected across the Gadwal schist belt in the state of Andhra Pradesh show an 8.4 mgal residual gravity anomaly associated with meta-sediments/volcanics of the linear NNW-SSE trending schist belt that shows metamorphism from green schist to amphibolite facies. This schist belt is flanked on either side by the peninsular gneissic complex. The elevation and slab Bouguer corrected residual gravity profile data were interpreted using 2-D prism models. The results indicate a synformal structure having a width of 1.8 km at the surface, tapering at a depth of about 2.6 km with a positive density contrast of 0.15 gm/cc with respect to the surrounding peninsular gneissic complex.

  6. Two new basaltic asteroids in the Outer Main Belt

    CERN Document Server

    Duffard, R

    2007-01-01

    The identification of other basaltic objects in the asteroid belt is mandatory to explain the diversity in the collection of basaltic meteorites. This diversity requires more than one differentiated parent body, a fact that is consistent with the diversity of differentiated parent bodies implied by the iron meteorites. Based on a list of previously identified candidate basaltic (V-type) asteroids, two asteroids in the outer main belt, (7472) Kumakiri and (10537) 1991 RY16, were spectroscopically observed during an observational run in Calar Alto Observatory, Spain. We confirm the V-type character of these two asteroids that, together with (1459) Magnya, become the only known traces of basaltic found in the outer main belt up to now. We also demonstrate that the searching for candidate V-type asteroids using a photometric survey, like the Sloan Digital Sky Survey, produces reliable results.

  7. The Living with a Star Radiation Belt Storm Probes

    Science.gov (United States)

    Sibeck, D. G.; Mauk, B. H.; Grebowsky, J. M.; Fox, N. J.

    2007-01-01

    The goal of NASA's Living With a Star Radiation Belt Storm Probe mission is to understand, ideally to the point of predictability, how populations of relativistic electrons and ions in space form or change in response to the variable inputs of energy from the Sun. The investigations selected for this 2-spacecraft mission scheduled for launch in early 2012 address this task by making extensive observations of the plasma waves, thermal, ring current, and relativistic particle populations, and DC electric and magnetic fields within the Earth's inner and outer radiation belts. We first describe the current mission concept within the scope of NASA's strategic plan and the Vision for Exploration, and then consider how its observations will be used to define and quantify the processes that accelerate, transport, and remove particles in the Earth's radiation belts.

  8. Recent Developments in the Radiation Belt Environment Model

    Science.gov (United States)

    Fok, M.-C.; Glocer, A.; Zheng, Q.; Horne, R. B.; Meredith, N. P.; Albert, J. M.; Nagai, T.

    2010-01-01

    The fluxes of energetic particles in the radiation belts are found to be strongly controlled by the solar wind conditions. In order to understand and predict the radiation particle intensities, we have developed a physics-based Radiation Belt Environment (RBE) model that considers the influences from the solar wind, ring current and plasmasphere. Recently, an improved calculation of wave-particle interactions has been incorporated. In particular, the model now includes cross diffusion in energy and pitch-angle. We find that the exclusion of cross diffusion could cause significant overestimation of electron flux enhancement during storm recovery. The RBE model is also connected to MHD fields so that the response of the radiation belts to fast variations in the global magnetosphere can be studied.Weare able to reproduce the rapid flux increase during a substorm dipolarization on 4 September 2008. The timing is much shorter than the time scale of wave associated acceleration.

  9. Radiation Belt Environment Model: Application to Space Weather and Beyond

    Science.gov (United States)

    Fok, Mei-Ching H.

    2011-01-01

    Understanding the dynamics and variability of the radiation belts are of great scientific and space weather significance. A physics-based Radiation Belt Environment (RBE) model has been developed to simulate and predict the radiation particle intensities. The RBE model considers the influences from the solar wind, ring current and plasmasphere. It takes into account the particle drift in realistic, time-varying magnetic and electric field, and includes diffusive effects of wave-particle interactions with various wave modes in the magnetosphere. The RBE model has been used to perform event studies and real-time prediction of energetic electron fluxes. In this talk, we will describe the RBE model equation, inputs and capabilities. Recent advancement in space weather application and artificial radiation belt study will be discussed as well.

  10. Relativistic surfatron process for Landau resonant electrons in radiation belts

    CERN Document Server

    Osmane, A

    2014-01-01

    Recent theoretical studies of the nonlinear wave-particle interactions for relativistic particles have shown that Landau resonant orbits could be efficiently accelerated along the mean background magnetic field for propagation angles $\\theta$ in close proximity to a critical propagation $\\theta_\\textrm{c}$ associated with a Hopf--Hopf bifurcation condition. In this report, we extend previous studies to reach greater modeling capacities for the study of electrons in radiation belts by including longitudinal wave effects and inhomogeneous magnetic fields. We find that even though both effects can limit the surfatron acceleration of electrons in radiation belts, gains in energy of the order of 100 keV, taking place on the order of ten milliseconds, are sufficiently strong for the mechanism to be relevant to radiation belt dynamics.

  11. RATIONALE FOR CENTERING CAPACITY OF REDISIGNED BELT CONVEYOR DRUMS

    Directory of Open Access Journals (Sweden)

    V. V. Suglobov

    2016-02-01

    Full Text Available Purpose. In the study is necessary: 1 to justify aligning drums of a new design of belt conveyors; 2 to develop a method for calculating and determining the rational design parameters of drums depending on the technical parameters of the conveyor belt (the length of the conveyor, belt width, the performance of the conveyor, the diameter of the drive and tension drums, etc.; 3 to carry out pilot studies of efficiency conveyor belt in a production environment in order to determine the magnitude of dynamic loads and a comparative evaluation of the effectiveness of the centering ability of conventional and new designs of drums. Methodology. To substantiate the effectiveness of the centering ability of the drums of a new design by the authors developed a mathematical model of interaction of the tape with the drum. Mathematical simulation of tape reels with new design comes to drawing up a differential equation of the belt based on the dynamic component and restoring force. This model allowed us to estimate the movement of the tape in the transverse direction based on the calculated additional dynamic loads and forces on the investigated centering a conveyor belt with given specifications. For the first time the technique of calculating and determining the rational parameters of the drums, which allows determining the design parameters of the centering portions, depending on the mechanical properties and geometric parameters of the tape. Findings. With the help of mathematical modeling the scientifically substantiated effect of centering the ability of the new design of the drum, which ensures stable tape running along the longitudinal axis of the conveyor. The authors made the following conclusions: 1 the mathematical model of interaction with the new belt design of the drum, which allowed to describe the belt in the transverse direction in view of additional dynamic loads and renewable power was developed; 2 the method of calculation and

  12. Central line infections - hospitals

    Science.gov (United States)

    ... infection; Central venous catheter - infection; CVC - infection; Central venous device - infection; Infection control - central line infection; Nosocomial infection - central line infection; Hospital acquired ...

  13. Histopathological investigations of rabbit colibacteriosis, caused by enteropathogenic escherichia coli (O15:H-

    Directory of Open Access Journals (Sweden)

    Petrov Vladimir

    2007-01-01

    Full Text Available The histopathological alterations in the intestinal tract of recently weaned rabbits with experimental and spontaneous Eschercihia coli (O15:H- infection were followed. A considerable shortening and thickening of well epithelized intestinal villi were observed, whose tips, after a Warthin-Starry staining, were profusely colonized with coliform bacteria. The observed pathological pattern was a permanent finding in such infections and could be used as a pathognomic feature in the differential diagnosis of spontaneous diarrhoeic syndromes. The adhesion of colibacteria to enterocytes, together with the data from the bacteriological studies (isolation, identification, determination of the O-serogroup affiliation and the biochemical behaviour allowed the assignment of isolates to the group of enteropathogenic E. coli (EPEC. Only when required, more detailed diagnostical procedures as PCR, could be performed.

  14. Aqueous alteration on main-belt asteroids

    Science.gov (United States)

    Fornasier, S.; Lantz, C.; Barucci, M.; Lazzarin, M.

    2014-07-01

    The study of aqueous alteration is particularly important for unraveling the processes occurring during the earliest times in Solar System history, as it can give information both on the thermal processes and on the localization of water sources in the asteroid belt, and for the associated astrobiological implications. The aqueous alteration process produces the low temperature (< 320 K) chemical alteration of materials by liquid water which acts as a solvent and produces materials like phyllosilicates, sulphates, oxides, carbonates, and hydroxides. This means that liquid water was present in the primordial asteroids, produced by the melting of water ice by heating sources, very probably by ^{26}Al decay. Hydrated minerals have been found mainly on Mars surface, on primitive main-belt asteroids (C, G, B, F, and P-type, following the classification scheme by Tholen, 1984) and possibly also on few transneptunian objects. Reflectance spectroscopy of aqueous altered asteroids shows absorption features in the 0.6-0.9 and 2.5-3.5-micron regions, which are diagnostic of, or associated with, hydrated minerals. In this work, we investigate the aqueous alteration process on a large sample of 600 visible spectra of C-complex asteroids available in the literature. We analyzed all these spectra in a similar way to characterize the absorption-band parameters (band center, depth, and width) and spectral slope, and to look for possible correlations between the aqueous alteration process and the asteroids taxonomic classes, orbital elements, heliocentric distances, albedo, and sizes. We find that 4.6 % of P, 7.7 % of F, 9.8 % of B, 50.5 % of C, and 100 % of the G-type asteroids have absorption bands in the visible region due to hydrated silicates. Our analysis shows that the aqueous alteration sequence starts from the P-type objects, practically unaltered, and increases through the P → F → B → C → G asteroids, these last being widely aqueously altered, strengthening thus

  15. The Belt voice: Acoustical measurements and esthetic correlates

    Science.gov (United States)

    Bounous, Barry Urban

    This dissertation explores the esthetic attributes of the Belt voice through spectral acoustical analysis. The process of understanding the nature and safe practice of Belt is just beginning, whereas the understanding of classical singing is well established. The unique nature of the Belt sound provides difficulties for voice teachers attempting to evaluate the quality and appropriateness of a particular sound or performance. This study attempts to provide answers to the question "does Belt conform to a set of measurable esthetic standards?" In answering this question, this paper expands on a previous study of the esthetic attributes of the classical baritone voice (see "Vocal Beauty", NATS Journal 51,1) which also drew some tentative conclusions about the Belt voice but which had an inadequate sample pool of subjects from which to draw. Further, this study demonstrates that it is possible to scientifically investigate the realm of musical esthetics in the singing voice. It is possible to go beyond the "a trained voice compared to an untrained voice" paradigm when evaluating quantitative vocal parameters and actually investigate what truly beautiful voices do. There are functions of sound energy (measured in dB) transference which may affect the nervous system in predictable ways and which can be measured and associated with esthetics. This study does not show consistency in measurements for absolute beauty (taste) even among belt teachers and researchers but does show some markers with varying degrees of importance which may point to a difference between our cognitive learned response to singing and our emotional, more visceral response to sounds. The markers which are significant in determining vocal beauty are: (1) Vibrancy-Characteristics of vibrato including speed, width, and consistency (low variability). (2) Spectral makeup-Ratio of partial strength above the fundamental to the fundamental. (3) Activity of the voice-The quantity of energy being produced. (4

  16. An Empirical Analysis of Farmers’ Rabbit Breeds Purchase and Its Influencing Factors

    Institute of Scientific and Technical Information of China (English)

    Yuhe; SONG; Laping; WU

    2014-01-01

    In this paper,based on the survey data on farmers in 14 provinces and cities nationwide provided by China Rabbit Research System,we analyze the farmers’ rabbit breeds selection,purchase channels and the demand for new varieties of rabbits as well as the problems in the course of rabbit usage. We make an empirical analysis of the factors influencing farmers’ rabbit demand,and put forth the recommendations for farmers’ rabbit breeds usage and to improve the promotion of new varieties of rabbits.

  17. Intestinal stricture following seat belt injury in children.

    Science.gov (United States)

    Lynch, J M; Albanese, C T; Meza, M P; Wiener, E S

    1996-10-01

    The most commonly reported intestinal injury from seat belts in children is perforation. A rarely reported late sequela following this type of injury is posttraumatic intestinal stricture (PTIS). A review of the literature reveals a common clinical pattern of presentation in children and adults but an apparent difference in the pathophysiologic mechanism between the pediatric and adult patient. Recently, we treated two children with PTIS. Each case is discussed, and a pathophysiological mechanism for this injury in children is proposed. Recommendations are made for the evaluation and treatment of these uncommon complications of seat belt-related blunt intestinal injury.

  18. Seat belt trauma: pectoralis muscle rupture and delayed mesh repair.

    Science.gov (United States)

    Harvey, Kyle P; Adair, James D; Ali, M Azhar

    2008-03-01

    To our knowledge, this is the first reported case of a seat belt-related rupture of the pectoralis major muscle and its successful delayed repair using mesh. We report a case of a 34-year-old white man who sustained a right pectoralis major muscle rupture from a seat belt during a motor vehicle crash. The patient presented to us 2 years after the injury. We introduce a technique using mesh that results in a successful repair of a cosmetically disfiguring chest wall defect.

  19. Photometry and Lightcurve Analysis of 7 Main-Belt Asteroids

    Science.gov (United States)

    Violante, Renata; Leake, M. A.

    2013-01-01

    We report the synodic periods and lightcurves for three main-belt asteroids and provide lightcurves for four other main-belt asteroids. 676 Melitta has a period of 8.35 ± 0.05 hours, with an amplitude of 0.056 ± 0.026 magnitude; 688 Melanie has a period of 16.10 ± 0.05 hours, and an amplitude of 0.091 ± 0.019 magnitude; 1677 Tycho Brahe has a period of 3.89 ± 0.06 hours, and an amplitude of 0.564 ± 0.011 magnitude.

  20. Active Asteroids: Main-Belt Comets and Disrupted Asteroids

    CERN Document Server

    Hsieh, Henry H

    2015-01-01

    The study of active asteroids has attracted a great deal of interest in recent years since the recognition of main-belt comets (which orbit in the main asteroid belt, but exhibit comet-like activity due to the sublimation of volatile ices) as a new class of comets in 2006, and the discovery of the first disrupted asteroids (which, unlike MBCs, exhibit comet-like activity due to a physical disruption such as an impact or rotational destabilization, not sublimation) in 2010. In this paper, I will briefly discuss key areas of interest in the study of active asteroids.