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Sample records for beckman coulter stemone

  1. BECKMAN COULTER HMX全自动五分类血细胞分析仪的临床应用

    Institute of Scientific and Technical Information of China (English)

    王江峰

    2016-01-01

    目的:对BECKMAN COULTER HMX全自动血细胞分析仅的主要性能进行评价.方法:我们安昭<全国临床检验操作规程>及贝克曼公司提供的方法,对ECKMAN COULTER HMX全自动五分类血细胞分析仪的精密度、准确度,线性范围和交叉污染率进行测定并和SYSMEX KX-21进行比较.结果:BECKMAN COULTER HMX全自动五分类血细胞分析仪精密度、准确度高,稳定性好,线性范围宽,交叉污染率低.结论:BECKMAN COULTER HMX全自动五分类血细胞分析仪是一台较理想的血细胞分析仪,适合在检验科中使用.

  2. BECKMAN COULTER HMX全自动五分类血细胞分析仪的临床应用

    Institute of Scientific and Technical Information of China (English)

    高旭红

    2011-01-01

    目的 对BECKMAN COULTER HMX 全自动血细胞分析仪的主要性能进行评价.方法 我们安昭及贝克曼公司提供的方法,对ECKMAN COULTER HMX 全自动五分类血细胞分析仪的精密度、准确度,线性范围和交叉污染率进行测定并和SYSMEX KX-21 进行比较.结果 BECKMAN COULTER HMX 全自动五分类血细胞分析仪精密度、准确度高,稳定性好,线性范围宽,交叉污染率低.结论 BECKMAN COULTER HMX 全自动五分类血细胞分析仪是一台较理想的血细胞分析仪,适合在检验科中使用.

  3. BECKMAN COULTER HMX全自动五分类血细胞分析仪的临床应用

    Institute of Scientific and Technical Information of China (English)

    王江峰; 杨刚

    2010-01-01

    目的:对BECKMAN COULTER HMX全全自动血细胞分析仪的主要性能进行评价。方法:我们安昭〈全国临床检验操作规程〉及贝克曼公司提供的方法,对ECKMAN COULTER HMX全自动五分类血细胞分析仪的精密度、准确度,线性范围和交叉污染率进行测定并和SYSMEXKX-21进行比较。结果:BECKMAN COULTER HMX全全自动五分类血细胞分析仪精密度、准确度高,稳定性好。线性范围宽,交叉污染率低。结论:BECKMAN COULTER HMX全自动五分类血细胞分析仪是一台较理想的血细胞分析仪,适合在检验科中使用。

  4. [Accreditation of automated complete blood count by the LH750 Analyzer (Beckman Coulter) in Georges Pompidou Hospital (Paris, France)].

    Science.gov (United States)

    Robinet, Sylvain; Lemaire, Pierre; Louis, Gauthier; Vieillefond, Vincent; Daigneau, Yolande; Gaillaud, Emilie; Vincent, Béatrice; Fischer, Anne-Marie; Siguret, Virginie

    2013-01-01

    Preliminary evaluation of quantitative clinical laboratory measurements is a prerequisite for the accreditation of clinical laboratories, according to the French Committee of Accreditation guidelines following the European reference Standard EN ISO 15189. Numerous papers have been published regarding biochemistry and immunology. However, data are lacking for automated complete blood count accreditation. We report here our experience at Hôpital européen Georges Pompidou hematology laboratory and present the performance characteristics of two mirrored LH750 Beckman-Coulter analysers, including precision, accuracy and uncertainty of measurement.

  5. Beckman Coulter ACCESS全自动微粒子化学分析仪的故障处理及应用体会

    Institute of Scientific and Technical Information of China (English)

    丁小红

    2006-01-01

    我科2004年4月购进美国Beckman Coulter ACCESS全自动微粒子化学发光免疫分析仪,该设备运用先进的方法学,高质量的且具有专利的包装试剂,全英文操作界面,操作流程合理,检测速度快,结果具有高灵敏、高准确性、高特异性、重复性好等特点,运行一年来得到临床科室的认可.现将使用过程中常见的故障及处理、一些应用经验总结如下,以供同行参考。

  6. Impact of the new Beckman Coulter Cytomics FC 500 5-color flow cytometer on a regional flow cytometry clinical laboratory service.

    Science.gov (United States)

    Luider, J; Cyfra, M; Johnson, P; Auer, I

    2004-01-01

    Calgary Laboratory Services (CLS) in Alberta, Canada, is the regional reference laboratory providing flow cytometry services for southern Alberta and southeastern British Columbia. As a busy reference flow laboratory we provide flow cytometry immunophenotyping for investigation and diagnosis of acute and chronic leukemias, lymphomas, immunodeficiencies, neuroblastoma, platelet disorders, and interstitial lung disease (ILD). Because of increasing workload and the continual effort to improve the service to our health care providers, CLS invested in the new Beckman Coulter Cytomics FC 500 5-color flow cytometer. In addition to time and labor savings due to reduced maintenance and operating system design, this new flow cytometer automates many of the previous manual steps involved in quality control and flow cytometric analysis. It also incorporates 2 lasers and is capable of measuring 5-color antibody combinations in a single tube, enabling us to reduce the number of tubes and overall costs, giving us better gating options for minimal residual disease analysis. We present the first published evaluation, an assessment of the overall productivity and cost impact of the new state-of-the-art Cytomics FC 500 flow cytometer. Implementation of the Cytomics FC 500 has resulted in a 20% reduction in reagent costs and shorter turnaround time for analysis and diagnosis. This instrument has allowed us to reduce our acute leukemia panel from 17 to 13 tubes, our lymphoma panel from 13 to 7 tubes, and our ILD panel from 4 to 2 tubes. The availability of 2 lasers provides more flexibility in choosing antibodies and conjugates to customize immunophenotyping panels. It also allows us to use the DRAQ5 dye and simultaneously analyze the immunophenotype and DNA content of cells with very little compensation. Many of the arduous, time-consuming flow operator tasks often associated with previous generation flow cytometry instruments, such as color compensation, list mode analysis, sample

  7. Microchip Coulter particle counter

    DEFF Research Database (Denmark)

    Larsen, Ulrik Darling; Blankenstein, Gert; Branebjerg, J.

    1997-01-01

    This paper presents a micro device employing the Coulter principle for counting and sizing of living cells and particles in liquid suspension. The microchip Coulter particle counter (μCPC) has been employed in a planar silicon structure covered with glass, which enables detailed observation during...

  8. Beckman coulter ACCESS定标失败的常见原因

    Institute of Scientific and Technical Information of China (English)

    韩绪娥; 黄爱华; 张园园; 崔丽娟; 宜永刚

    2006-01-01

    ACCESS全自动微粒子化学发光免疫分析仪.是由美国BECKMANCOULTER公司和法国PASTURE研究院合作设计生产的。它最大限度的拓展了临床免疫检测的范围.使临床免疫学检测全自动化的梦想成为现实。我院自2003年购进该仪器以来。以快速准确的结果为临床提供了高质量的服务。现将定标中遇到的问题介绍如下。

  9. Sensor and control for consistent seed drill coulter depth

    DEFF Research Database (Denmark)

    Kirkegaard Nielsen, Søren; Nørremark, Michael; Green, Ole

    2016-01-01

    of a drill coulter, providing sensor feedback to a control system via an electro hydraulic actuator that delivers an even coulter depth. The results showed a strong correlation between the angle of the coulter and the coulter depth under static (R^2=0.9996) and dynamic (R^2=0.987) operations, verified...

  10. Initial performance evaluation of the UniCel® DxH 800 Coulter® cellular analysis system.

    Science.gov (United States)

    Hedley, B D; Keeney, M; Chin-Yee, I; Brown, W

    2011-02-01

    The Beckman Coulter UniCel® DxH 800 is a hematology analyzer incorporating new electronic and mechanical design with advanced algorithm technology to perform CBC, white blood cell (WBC) differential, nucleated red blood cell (NRBC), and reticulocyte analysis. Evaluation of this instrument was performed in our 800-bed tertiary care hospital and specifically centered upon the correlation of WBC, NRBC, and platelet (PLT) enumeration when compared to a predicate analyzer, the Coulter® LH 780, and flow cytometry (FCM) reference methods. Of particular interest were those samples with morphologically confirmed interference and extreme leukocytosis (evaluated with respect to red blood cell parameter correction). The sample set (n=272) consisted of morphologically normal and hematologically abnormal patients. Correlation of the WBC, PLT, and NRBC showed r(2) values of 0.994, 0.985, and 0.910 for the DxH 800 vs. FCM, respectively. The presence of interfering particles did not affect the accuracy of the DxH 800 with respect to WBC counts. The DxH 800 showed accurate PLT and NRBC counts in the clinically significant low range when compared to FCM. Compared to the LH 780, flagging rates were significantly reduced (NRBC flag), or equivalent (WBC, PLT flag) on the DxH 800. The DxH 800 demonstrated higher sensitivity and specificity for PLTs and NRBCs and achieved a lower NRBC false negative rate compared to the LH 780. The UniCel® DxH 800 represents a significant improvement to previous impedance analyzers in accurately detecting the presence of NRBCs at counts >1/100 WBC. Furthermore, it provides accurate PLT and WBC counts in the presence of interference and improved NRBC flagging efficiency when compared to the LH 780. Correction of red blood cell parameters is appropriate and accurate in cases of extreme leukocytosis.

  11. Wallace H. Coulter: decades of invention and discovery.

    Science.gov (United States)

    Robinson, J Paul

    2013-05-01

    Only a few inventors can be said to have made as great an impact on mankind as Wallace Coulter. His inquisitive mind and ability to see well beyond what existed served him well for 40 years of inventing. So many of the fundamental tools that exist today in the area of hematology were derived from or driven by Coulter's inventions that he could be called the most technological innovator in the field of modern hematology. In achieving these discoveries Wallace Coulter was clearly capable of visualizing future opportunities that few others recognized. His vision was combined with an uncanny ability to translate his ideas into products. He developed a large number of tools that shaped the fields of cytometry, image analysis, and industrial materials. His understanding of the future power of computation drove him to link these technologies in a unique way. In the end, Coulter shaped the technologies that ultimately drove hematology in a new direction, one that remains on a critical pathway linking technology innovation all the way to true translational impact. It was said of Henry Ford that "[h]e has no notion that wealth has made him great, and any one who is imprest merely by his wealth bores him. In his personal contacts he likes to dodge the subject. He would prefer to talk with a machinist about machinery, or with somebody who likes birds about birds. In these contacts, he asks no deference; and if he gets it, he suspects it is mere deference to wealth, and that ends his interest."(1) The same could be said of Wallace Coulter, who, like Ford, understood the concepts of mass production and customer service. Coulter had the ability to recognize the opportunity and fulfill the need for development of a blood-cell counter that could be placed in every pathology laboratory, and in so doing transformed a field from a qualitative to a quantitative environment. Every person who has ever entered a medical lab, hospital, or doctor's office has felt the impact of Coulter

  12. From pro-prostate specific antigen, [-2]pro-prostate specific antigen to Beckman Coulter phi: the evolution of new biomarkers for early detection of prostatic carcinoma

    Institute of Scientific and Technical Information of China (English)

    ZHANG Ming-zhi; LU Yi-ping

    2012-01-01

    Prostate specific antigen (PSA) has a wide clinical use for the early detection of prostatic carcinoma (PCa); however,it has never been a perfect marker due to its low specificity and low positive predictive value which ranges between 4 ng/ml and 10 ng/ml.The discovery of different PSA molecular forms in serum in the early 1990s brought insight into searching for more specific markers.Since then free PSA (fPSA) has been used routinely to increase the specificity for PCa and to reduce unnecessary biopsies.More recently,promising data is emerging regarding one proenzyme molecular form of free PSA,proPSA,and a few truncated proPSA isoforms.The purpose of this article is to review the recent studies on clinical utility of proPSA,especially [-2]pPSA,an isoform of proPSA,and parameters involving [-2]pPSA as well as other PSA derivatives in early detection of PCa.

  13. Measurements of HbA1c by High Performance Liquid Chromatography in D-10 analyzer and Immunological Method by Beckman Coulter AU480 System: A Comparative Study

    Directory of Open Access Journals (Sweden)

    Md Aminul Haque Khan

    2012-07-01

    Full Text Available Background: HbA1c can be measured by different methods. Enzymatic, boronate affinity chromatographic and cation-exchange high performance liquid chromatographic (HPLC methods are considered as gold standard methods. To make the test cost-effective many laboratories use the immunological method installed in a chemistry analyzer. Objective: To compare the values of HbA1c measured by high performance liquid chromatographic method and immunological method in two laboratory settings. Materials and Methods: This observational and comparative study was conducted in Bangladesh Institute of Health Sciences (BIHS and Enam Medical College, Savar, Dhaka. HbA1c levels measured by HPLC in BIHS were compared with that measured by immunological method in Enam Medical College, Savar, Dhaka. p values 10.0% group, but it was within acceptable limit. Conclusion: HbA1c can be measured by immunological method installed in an automated chemistry analyzer to make the test cost-effective. But HPLC method is preferable.

  14. Laboratory evaluation of the Beckman Synchron CX3 clinical chemistry analyzer.

    Science.gov (United States)

    Peake, M J; Pejakovic, M; White, G H

    1988-02-01

    In this evaluation of the Beckman Synchron CX3, the multi-analyte clinical chemistry analyzer exhibited high precision, good linearity, and no carryover for each of the eight analytes measured. Results obtained correlated well with those produced by our routine instrumentation (Beckman Astra, Varian atomic absorption spectrophotometer). The instrument can process up to 75 samples per hour (600 tests per hour if all tests available are requested) and, after calibration, can provide urgent results for the complete panel of tests within 2 1/2 min. The performance characteristics of this instrument make it ideal as a routine or a "stat" analyzer for commonly requested tests in the clinical chemistry laboratory.

  15. Positive predictive values of abused drug immunoassays on the Beckman Synchron in a veteran population.

    Science.gov (United States)

    Dietzen, D J; Ecos, K; Friedman, D; Beason, S

    2001-04-01

    The pressure to reduce the cost of analytic testing makes it tempting to discontinue routine confirmation of urine specimens positive for drugs of abuse by immunoassay. Beyond the economic motivation, the requirement for confirmation should be driven by the positive predictive value of the screening tests. We have quantitated positive predictive values of our screening immunoassays in a large metropolitan Veterans Affairs Medical Center. We reviewed the confirmatory rate of urine specimens positive for drugs of abuse with Beckman Synchron reagents from June 1998 to June 1999 and tabulated the false-positive screening rate. There were 175 instances of false-positive screens during the 13 months we analyzed. Positive predictive values ranged from 0% (amphetamine) to 100% (THC). We determined that the low positive predictive value of the amphetamine assay in our laboratory was primarily due to the use of ranitidine (Zantac). Urine specimens containing greater than 43 microg/mL ranitidine were positive in our amphetamine assay. This concentration is routinely exceeded in our patients taking ranitidine. In our clinical and analytic setting, the Beckman THC assay did not require confirmation. The positive predictive values of the Beckman opiate, cocaine, barbiturate, propoxyphene, and methadone immunoassays dictate routine confirmatory testing in specimens that screen positive for these substances. Finally, because of its extreme sensitivity to ranitidine, the Beckman amphetamine assay has little utility in our laboratory setting.

  16. Wavelet transform-based methods for denoising of Coulter counter signals

    Science.gov (United States)

    Jagtiani, Ashish V.; Sawant, Rupesh; Carletta, Joan; Zhe, Jiang

    2008-06-01

    A process based on discrete wavelet transforms is developed for denoising and baseline correction of measured signals from Coulter counters. Given signals from a particular Coulter counting experiment, which detect passage of particles through a fluid-filled microchannel, the process uses a cross-validation procedure to pick appropriate parameters for signal denoising; these parameters include the choice of the particular wavelet, the number of levels of decomposition, the threshold value and the threshold strategy. The process is demonstrated on simulated and experimental single channel data obtained from a particular multi-channel Coulter counter processing. For these example experimental signals from 20 µm polymethacrylate and Cottonwood/Eastern Deltoid pollen particles and the simulated signals, denoising is aimed at removing Gaussian white noise, 60 Hz power line interference and low frequency baseline drift. The process can be easily adapted for other Coulter counters and other sources of noise. Overall, wavelets are presented as a tool to aid in accurate detection of particles in Coulter counters.

  17. 使用Coulter GENS血细胞分析仪的一点认识

    Institute of Scientific and Technical Information of China (English)

    张秀琳; 陆永辉

    2002-01-01

    @@ 本文就Coulter GENS五分类能否完全替代手工白细胞分类作了探讨. 1 材料与方法 1.1 仪器与试剂美国Coulter GENS全自动血液细胞分析仪及其配套试剂;Olympus显微镜. 1.2 标本来源本院门诊、住院患者和450例健康体检者.

  18. "Comments on Coulter and Smith": The Issue of Authorial Surplus in Narrative Research

    Science.gov (United States)

    Smith, Michael W.

    2009-01-01

    Advocates of narrative research often contend that it is superior to nonnarrative research, both qualitative and quantitative, because they believe it is better able to represent multiple perspectives and foster multiple interpretations. The author of this comment article on Coulter and Smith (2009) draws on literary theory and an analysis of the…

  19. Label-free protein detection using a microfluidic Coulter-counter device

    DEFF Research Database (Denmark)

    Rodriguez-Trujíllo, Romén; Ajine, Mohammad Akram; Orzan, A.;

    2014-01-01

    A new method for measuring specific protein concentrations in solutions has been developed. The technique makes use of the Coulter effect for detecting and sizing of micro-scaled objects suspended in a buffer fluid. The method is completely label-free as it is only based on the electrical readout...

  20. "Comments on Coulter and Smith": Relational Ontological Commitments in Narrative Research

    Science.gov (United States)

    Clandinin, D. Jean; Murphy, M. Shaun

    2009-01-01

    In this comment article on Coulter and Smith (2009), the authors raise concerns that focusing exclusively on issues of representation may lead readers to misunderstandings about narrative research. The authors argue that narrative ways of thinking about the phenomena under study are interwoven with narrative research methodologies. Drawing on…

  1. "Comments on Coulter and Smith": Narrative Researchers as Witnesses of Injustice and Agents of Social Change?

    Science.gov (United States)

    Barone, Tom

    2009-01-01

    In commenting on Coulter and Smith (2009), the author explores issues related to the place of the political in education research and in literature, but especially in forms of narrative research that possess both scientific and literary dimensions. More specifically, the author examines four sets of issues related to the researching and writing of…

  2. The Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin.

    LENUS (Irish Health Repository)

    Byrne, Brendan

    2010-02-01

    Commercially available prolactin immunoassays detect macroprolactin to variable degrees. Best practice requires laboratories to assess the cross-reactivity of their prolactin assay with macroprolactin, and where appropriate, introduce a screen for the presence of macroprolactin. Our policy has been to reanalyse hyperprolactinaemic samples following polyethylene glycol (PEG) precipitation and to report the resultant value as the monomeric prolactin content of the sample. The goal of this study was to determine the need to continue PEG precipitation when prolactin measurements with the Wallac AutoDELFIA were replaced by the Beckman DxI 800.

  3. Data transfer from Beckman LS 5800 liquid scintillation counters to IBM personal computers.

    Science.gov (United States)

    Maan, A C

    1985-09-01

    This communication describes a short routine in BASICA for the IBM-PC, written to collect data from a Beckman liquid scintillation counter. In the form presented here the routine converts incoming bytes into separate lines and saves these lines in a file. There are many possible applications for further use of the data in these files. A few suggestions are given as to the format in which data can be stored and how to process these data automatically after all samples have been counted. The only hardware needed is an asynchronous communications adapter for the IBM-PC and an RS232 cable.

  4. CMOS-Compatible Silicon-Nanowire-Based Coulter Counter for Cell Enumeration.

    Science.gov (United States)

    Chen, Yu; Guo, Jinhong; Muhammad, Hamidullah; Kang, Yuejun; Ary, Sunil K

    2016-02-01

    A silicon-nanowire-based Coulter counter has been designed and fabricated for particle/cell enumeration. The silicon nanowire was fabricated in a fully complementary metal-oxide-semiconductor (CMOS)-compatible process and used as a field effect transistor (FET) device. The Coulter counter device worked on the principle of potential change detection introduced by the passing of microparticles/cells through a sensing channel. Device uniformity was confirmed by scanning electron microscopy and transmission electron microscopy. Current-voltage measurement showed the high sensitivity of the nanowire FET device to the surface potential change. The results revealed that the silicon-nanowire-based Coulter counter can differentiate polystyrene beads with diameters of 8 and 15 μm. Michigan Cancer Foundation-7 (MCF-7) cells have been successfully counted to validate the device. A fully CMOS-compatible fabrication process can help the device integration and facilitate the development of sensor arrays for high throughput application. With appropriate sample preparation steps, it is also possible to expand the work to applications such as rare-cells detection.

  5. Performance verification of Beckman Coulter AU5800 automatic biochemical analyzer%贝克曼库尔特AU5800全自动生化分析仪性能验证

    Institute of Scientific and Technical Information of China (English)

    卢旭

    2016-01-01

    目的:为保证临床实验室检验结果的准确和可靠,对贝克曼库尔特AU5800全自动生化分析仪进行性能验证.方法:根据ISO 15189要求,参照美国临床实验室标准化委员会(Clinical and Laboratory Standards Institute,CLSI)的仪器性能验证指南及CLIA'88法案评价标准,对该生化分析仪总蛋白(TP)、白蛋白(ALB)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、γ-谷氨酰转移酶(GGT)、肌酸激酶(CK)、乳酸脱氢酶(LDH)、尿素(BUN)、肌酐(CRE)、尿酸(UA)、总胆固醇(CHO)、甘油三酯(TG)、葡萄糖(GLU)、磷(P3+)、氯(Cl)16个常规生化检验项目的精密度、正确度、线性范围、参考区间进行性能评价.结果:批内精密度小于1/4CLIA'88要求范围,日间精密度小于1/3CLIA'88要求范围;正确度不超过1/2CLIA'88要求范围;各检测项目引用的线性范围、参考区间合适.结论:贝克曼库尔特AU5800全自动生化分析仪符合质量目标要求,可以较好地满足临床需求.

  6. Evaluation of the Beckman Synchron CX4 clinical chemistry analyzer in a hospital laboratory.

    Science.gov (United States)

    Ambus, T; Korogyi, N; DeCampos, F; Groom, B; Innanen, V T

    1990-01-01

    The Beckman Synchron CX4 random-access multianalyzer was evaluated in a medium sized hospital laboratory. The instrument does end-point, rate, and multipoint assays and carries on-board reagents for 24 tests. In addition to predefined tests, the instrument can be programmed for 100 user-defined tests; these are stored on the hard disk and can utilize up to three component reagents each. The throughput is 200 tests per hour. There is stat testing capability. In our evaluation, within-run and between-run precision and linearity were good, and no reagent carryover was detected. There was good correlation with the in-house methodology for the 19 tests evaluated. A disadvantage at the time of evaluation was interference by elevated bilirubin on creatinine, phosphorus, uric acid, and triglycerides. This problem of interference is being addressed by the manufacturer.

  7. Disturbance of Ultisol soil based on interactions between furrow openers and coulters for the no-tillage system

    Energy Technology Data Exchange (ETDEWEB)

    Francetto, T.R.; Alonço, A. dos S.; Brandelero, C.; Machado, O.D. da C.; Veit, A.A.; Carpes, D.P.

    2016-11-01

    The present study evaluated the effect of different associations between coulters and fertilizer furrow openers on soil disturbance, furrow depth and width, according to forward speed. The study was conducted on a farm in Santa Maria (Brazil/RS), in soil classified as sandy loam Ultisol. The experiment consisted of 24 combinations of treatments with three replications in a 2×3×4 factorial experiment. The combinations were formed by the interaction of the factors including: two types of furrow openers (hoe and double-disc), three types of coulters (no-coulter, smooth and offset fluted) and four levels of forward speed (1.11, 1.67, 2.22 and 2.78 m/s). Soil elevation and soil disturbance area profiles were obtained with the use of a micro profilometer, and disturbance values were calculated with the aid of computer software program Auto Cad. The disturbance area was not affected by speed; it was greater when using the hoe opener, and in association with the offset fluted coulter. Speed was inversely proportional to the depth of the furrows made by the hoe opener. Furthermore, the hoe caused the greatest furrow width (0.26 m) in comparison with the double-disc (0.24 m). The use of different coulters associated with furrow openers increased this variable (0.23 m for the no-coulter condition, 0.25 m with smooth and 0.26 m with offset fluted). The use of coulters combined with furrow openers reduces soil swelling, in approximately 8% for the smooth and 20% for the offset fluted. (Author)

  8. Short-term effects of whole-tree and stem-only harvesting on C and N fluxes in two Picea abies stands, Norway

    Science.gov (United States)

    Janne Kjønaas, O.; Clarke, Nicholas; Eldhuset, Toril; Hietala, Ari; Cross, Hugh; Holt Hanssen, Kjersti; Økland, Tonje; Lange, Holger; Frode Nordbakken, Jørn; Røsberg, Ingvald

    2015-04-01

    Tree harvest and different harvesting methods may affect the soil carbon (C) pool in forest ecosystems. In conventional stem-only timber harvesting (SOH), branches and tops that are left in the forests may contribute to the build-up of the soil carbon pool. In whole-tree harvesting (WTH), inputs of organic matter from branches and tops are strongly reduced. We established field experiments at Gaupen, SE and Vindberg, SW Norway, to study the short-term effects of SOH and WTH on processes affecting the accumulation and loss of soil C. Logging residues on the WTH plots were collected in piles that were removed after 6 months, rendering two sub treatments (WTH-pile and WTH-removal areas). We weighed selected trees and logging residues, surveyed understorey biomass production, quantified pre-harvest soil C and nutrient pools down to 30 cm. Soil respiration was measured and soil water sampled monthly during the growing season, while temperature and moisture were measured continuously. Organic and mineral horizons were incubated at different temperatures to estimate potential C and N mineralization, and deep sequencing of the ITS2 barcode region of fungal DNA was performed on the samples. Litterbags were deployed in the SOH plots. The logging residues amounted to 2.2-2.4 kg C m-2. At Gaupen, the mean in situ soil respiration rates increased following harvest with all treatments, but were significantly higher in WTH-pile and SOH relative to the WTH-removal areas in the first year as well as the fourth year of treatment. The former rates included aboveground decomposing needles and twigs but excluded coarser branches. The observed increase in the WTH-removal areas may be related to decomposing roots, as well as to increased C mineralization partly due to the higher soil temperatures following harvest. Soil temperature was the single most important factor explaining the variability in soil respiration rates over all treatments. At Vindberg, a decrease in soil respiration was

  9. Observation and analysis of the Coulter effect through carbon nanotube and graphene nanopores.

    Science.gov (United States)

    Agrawal, Kumar Varoon; Drahushuk, Lee W; Strano, Michael S

    2016-02-13

    Carbon nanotubes (CNTs) and graphene are the rolled and flat analogues of graphitic carbon, respectively, with hexagonal crystalline lattices, and show exceptional molecular transport properties. The empirical study of a single isolated nanopore requires, as evidence, the observation of stochastic, telegraphic noise from a blocking molecule commensurate in size with the pore. This standard is used ubiquitously in patch clamp studies of single, isolated biological ion channels and a wide range of inorganic, synthetic nanopores. In this work, we show that observation and study of stochastic fluctuations for carbon nanopores, both CNTs and graphene-based, enable precision characterization of pore properties that is otherwise unattainable. In the case of voltage clamp measurements of long (0.5-1 mm) CNTs between 0.9 and 2.2 nm in diameter, Coulter blocking of cationic species reveals the complex structuring of the fluid phase for confined water in this diameter range. In the case of graphene, we have pioneered the study and the analysis of stochastic fluctuations in gas transport from a pressurized, graphene-covered micro-well compartment that reveal switching between different values of the membrane permeance attributed to chemical rearrangements of individual graphene pores. This analysis remains the only way to study such single isolated graphene nanopores under these realistic transport conditions of pore rearrangements, in keeping with the thesis of this work. In summary, observation and analysis of Coulter blocking or stochastic fluctuations of permeating flux is an invaluable tool to understand graphene and graphitic nanopores including CNTs.

  10. A novel hemoglobin variant beta135(H13) Ala > Asp identified in an asymptomatic Korean family by direct sequencing: suggesting a new insight into Hb Beckman mutation.

    Science.gov (United States)

    Kim, S Y; Kim, G Y J; Jo, S A; Lee, E H; Cho, E H; Hwang, S H; Lee, E Y

    2010-02-01

    This article describes the clinical observation of a novel hemoglobin (Hb) variant found during the course of routine blood testing on a 61-year-old subject. The Hb variant was observed during HbA1c testing by ion-exchange high-performance liquid chromatography. Alkaline electrophoresis and DNA sequencing confirmed the presence of a new Hb variant, HBB:c.407C > A (p.Ala136Asp). This mutation has been reported to induce Hb Beckman variant in the Globin Gene Server. However, it was different from the only experimental report for Hb Beckman by Rahbar, Lee & Asmeron (p.Ala136Glu; Hb Beckman alpha2 beta2 135(H13) ala-to-glu: a new unstable variant and reduced oxygen affinity. Blood 78, 204a). And our case was asymptomatic with normal lab findings, while Rahbar et al.'s case showed the clinical manifestations of chronic anemia. This would be a report for a novel Hb variant suggesting new insight of Hb Beckman variant. This would be a report of a novel Hb variant suggesting new insights into Hb Beckman variant.

  11. Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry

    Directory of Open Access Journals (Sweden)

    Jennifer Pasquier

    2015-06-01

    Full Text Available The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp. The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading, we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.

  12. Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry.

    Science.gov (United States)

    Pasquier, Jennifer; Rioult, Damien; Abu-Kaoud, Nadine; Hoarau-Véchot, Jessica; Marin, Matthieu; Le Foll, Frank

    2015-06-24

    The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD) where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp). The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading), we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.

  13. Response monitoring and action limits: use of ADC numbers in understanding the operational characteristics of the Beckman ASTRA chemistry system.

    Science.gov (United States)

    Kessner, A; Burnett, R W; Bowers, G N

    1981-07-01

    The Beckman ASTRA is a microprocessor-controlled multichannel chemistry analyzer. The output of each module is available to the operator as analog-to-digital conversion (ADC) numbers, which we record during each calibration (Response Monitoring). After so studying three instruments for a total of 33 months, we have established limits for Calibrator ADC numbers that indicate possible operator action (Action Limits). These Action Limits are tighter than the microprocessor's programmed limits, and alert the operator to short- and long-term drift. These tighter limits warn of (a) impending failure of the instrument to calibrate or (b) possible inaccuracies in results for patients. We have instituted changes in preventive maintenance based on our studies of each module's operational characteristics, and have replaced electrodes that failed to meet our Response Monitoring specifications. Response Monitoring and Action Limits based upon ADC numbers have significantly enhanced our understanding of the ASTRA system and thus improved its operational efficiency and analytical reliability. Estimates of precision and accuracy (true value) were satisfactory in comparison to our prior single-channel continuous-flow and flame photometry analytical measurement systems.

  14. CRADA with Beckman Instruments and Pacific Northwest National Laboratory (PNL-013): Development and commercialization of the Unsaturated Flow Apparatus (UFA) using characterization of aridisols

    Energy Technology Data Exchange (ETDEWEB)

    Wright, J.; Conca, J.

    1996-10-01

    The objective of this Cooperative Research and Development Agreement (CRADA) was to develop and commercialize a technology conceived by scientists at Pacific Northwest National Laboratory (PNNL) and manufactured by Beckman Instruments, Inc. (Beckman), and to apply this technology to the characterization of and soils. The technology is the Unsaturated Flow Apparatus (UFA). The UFA provides a highly efficient method of direct, rapid measurement of hydraulic conductivity and other flow properties according to Darcy-Buckingham principles because the operator controls both the fluid driving force, using an ultracentrifuge, and the flow into the sample while it is spinning, with a rotating seal assembly. The concept of using centrifugation to significantly decrease the time needed, from years or months to days, for study of subsurface transport, particularly under unsaturated conditions, was conceived by James Conca, Ph.D., and Judith Wright, Ph.D., in 1986. The prototype UFA was developed in 1988 because there was a need to rapidly and accurately determine transport parameters in soils, sediments, and rocks for the Grout Waste Disposal Program. Transport parameters are critical to modeling outcomes for site-specific solutions to environmental remediation and waste disposal problems.

  15. 免耕播种机圆盘破茬刀工作性能分析与试验%Analysis and experiment on working performance of disc coulter for no-tillage seeder

    Institute of Scientific and Technical Information of China (English)

    白晓虎; 林静; 吕长义; 胡艳清

    2014-01-01

    Compared with traditional tillage, conservational tillage-also known as zero tillage can effectively prevent soil from wind and water erosion, resist spring drought, prevent sandstorm, increase crop yield, and save cost. No-tillage planter is an important agricultural implement for conservational tillage. In northeast China where ridge culture is the primary planting mode for maize production, the highly effective stubble-cutting device is required because the soil in this region is often covered by hard stubble and a small amount of stalks. Stubble-cutting device is one of the key components affecting performance of no-tillage planter. Two kinds of stubble-cutting device are commonly used, including power-driven device and passive one. Power-driven device can smash stubble and create good seedbed, but is also associated with soil disturbance, high power consumption and blade wear. Passive device of simple structures consumes less energy and only causes slight soil disturbance. Among them, rolling types of disc stubble-cutting coulters can easily cut through stubble under the soil surface without throwing stubbles out of the soil, so it is suitable for application in Northeast China. There are few studies on cutting velocity and working resistance of dis coulters. However, an understanding of cutting velocity and working resistance of dis coulters is necessary for design and application of dis coulters. Therefore, this study investigated the cutting velocity under the condition that coulters slides occurred, and working resistance in vertical direction as well as tractive resistance by kinematics and force analysis, the influence of auxiliary weight and unit working speed on stubble-cutting depth of three disc coulters (plain, wavy, and turbo) in single factor field experiments, and the influence of disc coulter type, auxiliary weight and unit working speed on tractor resistance in an orthogonal field experiment of three factors and three levels. Both theoretical

  16. "US-JAPAN CONFERENCE: INFLAMMATION, DIABETES AND CANCER" held at the Beckman Research Institute of City of Hope, Duarte, CA, USA.

    Science.gov (United States)

    Fujita-Yamaguchi, Yoko; Lin, Ren-Jang; Jove, Richard

    2011-12-01

    The conference was held to facilitate research collaborations between US and Japan scientists, and to commemorate the rich history of the Beckman Research Institute of City of Hope (COH) research contributed by many Japanese doctors. Most noticeable is Dr. Ryojun Kinoshita, an internationally renowned oncologist who built the first research team at the campus of COH Medical Center in 1952. The conference received enthusiastic support from Junichi Ihara, Consul General of Japan, Los Angeles. During the two day conference, seven scientists from Japan, six scientists from Southern California, and six scientists from COH presented various aspects of cancer and diabetes research which included nuclear receptor regulation, DNA base and chromatin modifications, cancer glycosylation, circadian clock, cell polarity, tumorigenesis, micro and small RNA therapies, genomics, epigenetics, and signaling.

  17. Measurement of isolated myocyte volume using the Coulter models Z2 and ZM/C256: a comparison of instrument function.

    Science.gov (United States)

    Said, S; Tamura, T; Gerdes, A M

    1998-09-01

    Changes in cardiac structure that depart from normal have generally been termed "remodeling". Assessment of ventricular remodeling at the cellular level should include measurement of myocyte dimensions. A well-established and reliable method to assess myocyte remodeling uses isolated cells and the Coulter Counter/Channelyzer system. The new Coulter Model Z2 has numerous modifications and improvements from the Model Z predecessor(s) interfaced to a pulse-height analyzer (e.g., channelyzer). Improvements of the Model Z2 over older instruments include: (i) elimination of the mercury manometer with accompanying oil-displacement pump; (ii) reduced size and weight; (iii) a higher degree of mechanization and automation; (iv) inclusion of an advanced comprehensive statistical package and (v) a substantial reduction in cost. The purpose of this study was to determine if the newly modified instrument produces the same results as the previous instrument combinations, which were shown to produce reliable cell volume data from irregularly shaped cells such as cardiac myocytes.

  18. 应用NCCLS文件对Beckman CX9全自动生化分析仪的精密度进行研究

    Institute of Scientific and Technical Information of China (English)

    莫凡

    2005-01-01

    目的对已使用了5年的Beckman CX9全自动生化仪进行精密度评价.方法通过测定K+、ALT、ALB、TG的含量,评价其批内精密度(Swr)、总精密度(ST).结果K+、ALT、ALB、TG高低浓度的Swr分别为0.23mmol/L、0.10mmol/L;1.82IU/L、0.90IU/L;1.94g/L、1.31g/;0.06mmol/L、0.02mmol/L;Sr分别为0.26 mol/L、0.12 mmol/L;1.62 IU/L、1.28 IU/L;1.77 g/L、1.23 g/L;0.08 mmol/L、0.03mmol/L;x2检验x2<x2(95%)P>0.05.结论该仪器使用5年后精密度依然良好.

  19. Evaluation of self-established assay system in Beckman DXC800 chemistry analyzer measured by prealbumin reagent kits%应用国产前白蛋白在Beckman上自建检测系统及其评价

    Institute of Scientific and Technical Information of China (English)

    张家云; 邓芳; 孙永梅; 李明

    2013-01-01

    目的:应用EP9-A对某国产前白蛋白在Beckman上的自建检测系统,并进行评价和偏差评估。方法:依据美国临床实验室标准化委员会(NCCLS)EP9-A应用北京利德曼前白蛋白(PA)试剂盒及其配套校准品、质控品在Beckman DXC800全自动生化分析仪的检测系统对40份患者新鲜血清的PA进行检测,并与Beckman公司原装配套的前白蛋白试剂盒(PAB)比对,对检测浓度进行系统评价和偏差分析。结果:北京利德曼前白蛋白(PA)试剂在Beckman DXC800全自动生化分析仪上的准确度、批内精密度、线性均符合要求,两种试剂检测结果无显著差异(P>0.05),具有较好的可比性,其相关系数(r2)>0.99。结论:自建检测系统血清PA检测结果与Beckman可溯源的参考检测系统相比,可被临床接受。%Objective:To evaluation the comparability and clinical acceptability of national prealbumin reagent kits measured by the self-established assay system in Beckman DXC800 chemistry analyzer.Methods:The levels of prealbumin in 40 patients' fresh serum, Calibrators and Controls were analyzed by self-established and Beckman ref-erence assay system. To compared with each other, the bias were estimated according to National Committee for Clini-cal Laboratory Standards (NCCLS) document EP9A2 files. Results:The trueness, inter-precision and linear of Beijing Leadman prealbumin reagent kits measured by the self-established assay system in Beckman DXC800 chemistry an-alyzer were acceptable. There was no statistically significant difference, P>0.05. The correlation coefficient (r2) was more than 0.99. Conclusion:The comparability of national prealbumin measured by the self-established assay system in Beckman DXC800 chemistry analyzer compared with Beckman reference assay system was acceptable.

  20. Analysis of the correlation of the results obtained by Vitros350 dry chemistry analyzer and Beckman AU5800 automatic biochemical analyzer%Vitros350干化学分析仪和Beckman AU5800全自动生化分析仪检测结果相关性分析

    Institute of Scientific and Technical Information of China (English)

    谢光素; 周琳; 陈华根

    2015-01-01

    ObjectiveAnalysis of the correlation of the results obtained by Vitros350 dry chemistry analyzer and Beckman AU5800 automatic biochemical analyzer.MethodsA random collection of 40 cases of hospitalized patients with qualified serum specimens,respectively with Vitros350 dry chemistry analyzer and Beckman AU5800 automatic biochemical analyzer analysis of total bilirubin,alanine aminotransferase,aspartate aminotransferase,total protein,albumin,gamma glutamyl transpeptidase,alkaline phosphatase,urea nitrogen,creatinine,uric acid,creatine kinase,creatine kinase isoenzyme, calcium,phosphorus,magnesium,carbon dioxide,amylase,lipase and glucose,a total of 19 projects,the results were statistically analyzed.ResultsExcept nine projects such as alanine aminotransferase,aspartate aminotransferase,albumin, urea nitrogen,creatinine,uric acid,amylase,lipase and glucose test results there was no statistically significant difference (P>0.5),statistically significant difference were observed in the rest of the project,relevant regression analysis showed that total bilirubin,total protein,gamma glutamyl transpeptidase and alkaline phosphatase linear good(r>0.975),creatine kinase, creatine kinase isoenzyme,magnesium,calcium,phosphorus,carbon dioxide,must expand the scope of valid data(r0.5),其余项目差异均具有统计学意义。相关性分析显示总胆红素、总蛋白、谷氨酰转肽酶、碱性磷酸酶线性关系良好(r>0.975),肌酸激酶、肌酸激酶同工酶、钙、无机磷、镁、二氧化碳必须扩大有效数据范围(r<0.975)。结论不同检测系统的仪器检测同一项目时,应进行相关性分析,对相关性较差的项目须立即采取措施,使结果具有可比性。

  1. Analysis the causes of suspicious results for BECKMAN PK 7300 automatic blood inspection device%BECKM AN PK7300全自动血型检测系统可疑结果的原因分析

    Institute of Scientific and Technical Information of China (English)

    刘淼; 王霞; 潘彤

    2016-01-01

    目的:探讨BECKMAN PK7300全自动血型检测系统在血站实验室用于血型筛查时出现结果可疑的原因以及应用中的注意事项。方法选取天津市血液中心2015年5月至2015年8月ABO及Rh血型经微板法鉴定后的无偿献血者的标本14417例,再由PK7300进行检测,统计分析ABO及Rh血型结果判读的正确率、错误率以及对可疑结果的原因进行分析。结果ABO血型结果一次性判读正确率为99.01%,失败率0.99%(其中正反定型不符0.48%),无错误判读;Rh(D)血型正确判读率100%。结论 BECKMAN PK7300全自动血型检测系统结果准确可靠,实现了血型检测的自动化、标准化,减少人为因素对实验的影响,反应图像可长期保存,保证结果的可溯性,节约成本,适用于血站实验室血型批量化检测需要。%Objective To investigate the causes of suspicious results for BECKMAN PK 7300 automatic blood inspection device on blood typing in blood station and the application notes .Methods A total of 11417 samples from unpaid blood donors were re‐cruited from Tianjin Blood Center between May 2015 and August 2015 .Microplate assay was applied in ABO and Rh(D)blood typ‐ing .All the samples were tested by BECKMAN PK7300 automatic blood inspection device .The accuracy and error rates were calcu‐lated for ABO and Rh(D)blood group and causes of suspicious results were analyzed .Results The accuracy and failure rate of ABO blood group were 99 .01% and 0 .99% (of which the inconsistent rate of positive and reverse ABO blood typing was 0 .48% )and there was no false reading .Its accuracy was 100% in Rh(D)blood grouping .Conclusion The results of BECKMAN PK7300 auto‐matic blood inspection device were accurate and reliable .The whole system achieved automation and standardization and reduced the artificial factors for experiments ,which images could be saved for a long period of time ,saving the cost .The

  2. Prostate Cancer Detection by Molecular Urinalysis

    Science.gov (United States)

    2011-04-01

    molecule 1 (ICAM-1), and neurotrophin-3 (NT-3). A Quantikine human immunoassay kit (R&D Systems, Minneapolis, MN) was used to detect interleukin 17 (IL17...Total PSA levels in each prostatic fluid sample were measured by Hybritech PSA assay on the Beckman Coulter Access Immunoassay System (BeckmanCoulter...chronic pelvic pain syndrome? J Urol 2006;176:119–24. 14. Schroder FH, Carter HB , Wolters T, van den Bergh RC, Gosselaar C, Bangma CH, Roobol MJ. Early

  3. Evaluation of the Tri-Service Laboratory System. Volume 1. Overview and Executive Summary.

    Science.gov (United States)

    1983-06-20

    Oakland had the SMAC (high-volume chemistry analyzer ), Coulter S+ (high volume * -instrument used in hematology) and Clintech instruments on-line. Wright...Patterson had only the Hycel (high-volume chemistry analyzer ) on-line. Eisenhower had the SMAC, ABA 200, Clintech, Beckman Astron, and Coulter S

  4. Enough room for Williams and IMF? / Paul Beckman

    Index Scriptorium Estoniae

    Beckman, Paul

    1999-01-01

    Lõppesid Leedu ja USA energeetikakompanii Williams International läbirääkimised Leedu naftakompleksis osaluse omandamise asjus. IMF uurib Leedu majanduslikku arengut, mida tehing Williamsiga komplitseerib

  5. DESEMPENHO OPERACIONAL DE UMA SEMEADORA ADUBADORA À TRAÇÃO ANIMAL, COM DIFERENTES SISTEMAS DE SULCADORES, REGULAGENS DE DISCO DE CORTE E COBERTURAS MORTAS, NO PLANTIO DIRETO DO FEIJOEIRO (Phaseolus vulgaris L. PERFOMANCE EVALUATION OF AN ANIMAL TRACTION PLANTER WITH DIFFERENT SOIL OPENER SYSTEMS, COULTER SETTINGS AND MULCHINGS FOR BLACK BEANS DIRECT DRILLING (Phaseoulus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Rogério de Araújo Almeida

    2007-09-01

    Full Text Available

    Em um experimento realizado na Escola de Agronomia da Universidade Federal de Goiás, em Goiânia (GO, no ano de 1998, fez-se a avaliação do desempenho operacional de uma semeadora-adubadora a tração animal, no plantio direto do feijoeiro (Phaseolus vulgaris L.. O experimento foi conduzido em um latossolo vermelho-escuro distrófico, textura média. Utilizaram-se dois tipos de cobertura morta, três sistemas de sulcador e duas regulagens para o disco de corte. O sistema de sulcador do tipo disco duplo defasado mobilizou menor volume de solo, exigiu menos potência no tracionamento e menor consumo de energia que os sistemas providos de sulcador do tipo facão.

    PALAVRAS-CHAVE: Plantio direto; tração animal; semeadora.

    In an essay, carried out in 1998, the performance of an animal traction planter for direct drilling of black beans (Phaseolus vulgaris L. was evaluated. The experiment was carried out in a distrofic dark red oxisol in the experimental fields of the Agronomy School of the Federal University of the State of Goiás, Brazil. Two levels of mulching, three soil opener systems, and two coulter settings were compared. Double disc opener reduced soil disturbance, required less power traction and energy.

    KEY-WORDS: Zero tillage; animal traction; planter machine.

  6. Força de tração e volume de solo mobilizado por haste sulcadora em semeadura direta sobre campo nativo, em função do teor de água no solo, profundidade e velocidade de operação Draft and soil loosening by knife type coulter related to soil moisture and planter's working speed and depth

    Directory of Open Access Journals (Sweden)

    Carla T. C. Cepik

    2005-08-01

    's coulters. The specific aims of the present work are to determine the knowledge of coulter draft requirements and the amount of displaced soil and tractor slippage on a Paleudult soil at different soil moisture condition, in direct planting on natural pastures chemically killed. Draft requirements increased with an increase in working depth. The increase of the ground speed had no effect on draft when soil was dry or moist; it increased however, on friable soil. Soil displacement was always higher with dry soil and at 12 cm working depth. When soil was friable or dry, the front wheels traction was needed to keep tractor slippage within acceptable levels.

  7. Identification of Biomarkers Associated with the Healing of Chronic Wounds

    Science.gov (United States)

    2008-01-01

    confidence to report. Trouble shooting with the mass spectrometry facility led to the use the Beckman Coulter ProteomeLab™ IgY-12 Proteome Partitioning...Tryptophan aspartate-containing coat protein) (TACO) - Homo sap 0.961136 1.001456 0.96953 0.664773 0.931996 0.863873 1.165928 1.11607 1.014085 cra ...hCP1609934.2| keratin 1 (epidermolytic hyperkeratosis) 0.669842 0.722419 0.951622 1.751633 1.824133 1.971052 1.179016 1.244589 1.221857 cra |hCP1812051

  8. Deciphering the Adaptive Immune Response to Ovarian Cancer

    Science.gov (United States)

    2014-10-01

    Cancer Society Research Institute Dates: 02/2014 – 01/2016 Term: 2 years PI: Brad Nelson Co-PIs: Rob Holt and Sohrab Shah The major goal of...ex vivo by staining with antibodies to CD4 or CD8 in combination with an anti-TCR Vb repertoire panel (IOTEST BetaMark; Beckman Coulter) and analyzed...for HLA-C14:03 are not currently available. Moreover, flow cytometry with an antibody to the Vb region used by clone 1 ( Vb 13.6) indicated that

  9. [Estimation of mescaline and pellotine in Lophophora coulter plants (Cactaceae) by means of the oscillographic polarography].

    Science.gov (United States)

    Gabermann, V

    1978-02-01

    Oscillographic polarography has been applied for the mescaline and pellotine estimation. These alkaloids produce in 0.5 N NaOH electrolyte a sharp peak within the cathode region of the oscillogram, each of them showing different potential. It makes possible to estimate them at a concentration of 5.10(-6) g/ml. All the forms of Lophophora williamsii were found to contain mescaline and lower content of pellothine, L. jourdaniana--to have equal content of both alkaloide, L. diffusa and L. fricii--to contain pellotine and only traces of mescaline. Plants grown in the greenhouse accumulated the same amount of alkaloids as native plants. Grafting on roodstock which does not produce essential amount of the alkaloids, does not affect the ability of Lophophora to synthesize mescaline and pellotine.

  10. Effect of soil texture and chemical properties on laboratory-generated dust emissions from SW North America

    Science.gov (United States)

    Mockford, T.; Zobeck, T. M.; Lee, J. A.; Gill, T. E.; Dominguez, M. A.; Peinado, P.

    2012-12-01

    Understanding the controls of mineral dust emissions and their particle size distributions during wind-erosion events is critical as dust particles play a significant impact in shaping the earth's climate. It has been suggested that emission rates and particle size distributions are independent of soil chemistry and soil texture. In this study, 45 samples of wind-erodible surface soils from the Southern High Plains and Chihuahuan Desert regions of Texas, New Mexico, Colorado and Chihuahua were analyzed by the Lubbock Dust Generation, Analysis and Sampling System (LDGASS) and a Beckman-Coulter particle multisizer. The LDGASS created dust emissions in a controlled laboratory setting using a rotating arm which allows particle collisions. The emitted dust was transferred to a chamber where particulate matter concentration was recorded using a DataRam and MiniVol filter and dust particle size distribution was recorded using a GRIMM particle analyzer. Particle size analysis was also determined from samples deposited on the Mini-Vol filters using a Beckman-Coulter particle multisizer. Soil textures of source samples ranged from sands and sandy loams to clays and silts. Initial results suggest that total dust emissions increased with increasing soil clay and silt content and decreased with increasing sand content. Particle size distribution analysis showed a similar relationship; soils with high silt content produced the widest range of dust particle sizes and the smallest dust particles. Sand grains seem to produce the largest dust particles. Chemical control of dust emissions by calcium carbonate content will also be discussed.

  11. Decreasing Quality of the New Generations of Anti-Müllerian Hormone Assays

    Directory of Open Access Journals (Sweden)

    Krzysztof Lukaszuk

    2014-01-01

    Full Text Available Anti-Müllerian hormone (AMH measurements are widely used to optimize the stimulation protocols. First generation AMH kits correlated well with ovarian reserve and response to stimulation. In the present study we aimed to asses if the new generation kits share the same accurate correlations. Retrospective data were collected from 8323 blood samples. For comparison we used Immunotech I generation kit (ImI 4035 samples, Beckman Coulter II generation kit RUO (BCII RUO 3449, samples and Beckman Coulter II generation kit with IVD certificate (BCII IVD 839 samples. We compared average AMH concentrations measured with different kits, as well as correlation between kits. We also compared average AMH concentrations in sera collected on different cycle days and samples of different quality of preservation. AMH serum concentrations differed for each kit, ranging 4.4 ± 4.12 (mean ± SD for the ImI, 2.68 ± 3.15 for the BCII RUO, and 1.64 ± 2.85 for BCII IVD. The mean differences from an adjusted regression model were −48.7%, −40%, and −69.2%, respectively. In conclusion, the changes of the BC AMH kits are unpredictable; however, the improvement of them is still possible. It would be very dangerous to use elaborated stimulation protocol (based on the Ist generation AMH results with the results from the IInd generation assays.

  12. Comparison of the Results of Sentive Cardiac Troponin I Detected by Two Different Detection Methods%两种不同方法检测敏感心肌肌钙蛋白Ⅰ结果比较

    Institute of Scientific and Technical Information of China (English)

    宋金萍; 孟晓辉; 达仁别克·苏利坦拜

    2012-01-01

      Objective:To comparise results of serum cardiac troponin I by Beckman-Coulter Access Immunoassay analyzer and Johnson VITROS-5600 fully automated dry chemistry analyzer,and exploring the differences as well as the linear relationship and correlation between the two. Method:45 serum samples were collected from patients presenting symptoms suggestive of acute myocardial infarction. The concentrations of serum cardiac troponin I were measured with Beckman-Coulter Access Immunoassay analyzer and Johnson VITROS-5600 fully automated dry chemistry analyzer. Data were processed by SPSS13.0 statistical analysis software.Result:Results of serum cardiac troponin I using the two methods showed no significant difference(P>0.05),while analysis shows the significant relation between the two methods(P0.05),组存在显著相关性(P<0.05).结论:本实验室采用的这两种敏感cTnI的检测结果一致性较好.

  13. Performance evaluation of the latest fully automated hematology analyzers in a large, commercial laboratory setting: a 4-way, side-by-side study.

    Science.gov (United States)

    Bourner, G; Dhaliwal, J; Sumner, J

    2005-01-01

    Gamma-Dynacare is a Canadian-based community laboratory partnership formed in the mid-1990s through the merger of 3 prominent Ontario medical diagnostic laboratories. Laboratory Corporation of America acquired an interest in the GD partnership in mid 2002. We service more than 10,000 community-based Canadian clinicians, hospital partners, and private clients with an integrated customer-focused system that includes specimen collection, transportation, and results reporting services. With more than 1,700 highly qualified medical, technical, and support staff and a network of laboratories, Gamma Dynacare aims to be at the forefront of technological innovation to better service the clinician base and ultimately deliver better patient care. We were looking for a hematology analyzer that would allow. (1) standardization throughout Ontario in our 4 largest sites and (2) better performance to effectively handle aged samples and minimize slide review. To select the best, most productive hematology analyzer for our environment, it was decided to perform a side-by-side comparison of the top hematology analyzers from Abbott (Cell-Dyn 3500), Beckman Coulter (LH 750), Bayer (Advia 120), and Sysmex (XE 2100), utilizing the same samples. CBC, differential and reticulocyte parameters were all evaluated according to CLSI (formerly NCCLS) and established hematology analyzer evaluation guidelines. We assessed each analyzer for precision, linearity, carryover, stability, differential capabilities, slide review rates, and throughput (clean bench studies). Two hundred samples were assessed for differential and morphology flagging on each analyzer using the reference 400 cell manual differential for comparison. Throughput was assessed by analyzing 700 consecutive samples representative of our workload mix. Stability studies at 24 hours showed that the Beckman Coulter LH 750 was least affected by EDTA, effect with minimal changes in the mean corpuscular volume (MCV) and hematocrit. Both

  14. MCV, MCH and SF in Pregnancy with Mediterranean Anemia of Clinical Application%MCV、MCH及SF在妊娠合并地中海贫血的临床应用

    Institute of Scientific and Technical Information of China (English)

    郑穗瑾; 章小东; 张秀果; 黄霄行; 王彩红

    2014-01-01

    Objective To investigate the MCV , MCH and serum ferritin in the diagnosis of thalassemia joint applica-tion.Methods In healthy control group of 60 patients with thalassemia heterozygous carriers 120 cases, the application Beckman.Coulter ( Beckman-Coulter ) LH750-type five categories of blood cell analyzer erythrocyte MCV , MCH.Serum ferritin by chemiluminescence.Results Thalassemia group and IDA group MCV , MCH were reduced , both with the healthy control group , the difference was statistically significant ( P<0.01 ) thalassemia group and IDA group., MCV thalassemia group was significantly lower than the IDA group , there was significant difference between the two ( P<0.01 ).IDA SF group decreased , while thalassemia group SF is not low , the difference between the two was statistical-ly significant (P<0.01).Conclusions (1)MCV, MCH and serum ferritin screening of pregnant women as an effective indicator of thalassemia;(2)do prenatal screening for thalassemia , to prevent and reduce heavy significance in children born with thalassemia.%目的:探讨MCV、MCH及血清铁蛋白联合应用在地中海贫血中的诊断意义。方法健康对照组60例,地中海贫血杂合子携带者120例,应用Beckman-Coulter (贝克曼-库尔特) LH750型五分类血细胞分析仪检测红细胞MCV、MCH。用化学发光法测定血清铁蛋白。结果地贫组和IDA组MCV、MCH 均减低,两者与健康对照组比较,差异均有统计学意义( P<0.01)。地贫组与IDA组比较,地贫组MCV较IDA组明显减低,两者之间差异有统计学意义( P<0.01)。 IDA组SF降低,而地贫组SF不低,两者之间差异有统计学意义( P<0.01)。结论(1)MCV、MCH及血清铁蛋白可作为筛查孕妇地贫的有效指标;(2)做好产前地贫的筛查,对预防和降低重型地贫患儿出生具有重要意义。

  15. PHASE STRUCTURE OF W-DOPED NANO-TiO2 PRODUCED BY SOL-GEL METHOD

    Institute of Scientific and Technical Information of China (English)

    Yucheng Wu; Xiaoye Hu; Ting Xie; Guanghai Li; Lide Zhang

    2005-01-01

    With Ti(OBu)4 as precursor, and HAc as complexing agent, pure and W-doped TiO2 gelatins were prepared by a sol-gel method. During the process of gel formation, metal ions were dispersed in the porous TiO2 matrix.Then, powders of nano-TiO2 and W-doped nano-TiO2 were prepared by drying, grinding and heat treatment at different temperatures. The grain size and structure of the samples, pure TiO2 and W-doped, and treated at different temperatures,were studied by X-ray diffraction (XRD), Beckman Coulter Sorption Analysis and TEM.Results showed that, with increasing temperature, the TiO2 transformed from anatase to rutile and the grain size increased. This transformation and grain growth of TiO2 could be retarded by doping with W.

  16. Comparison study of trapped water in human erythrocytes by EPC and DSC method and its application

    Institute of Scientific and Technical Information of China (English)

    ZHAO; Gang; HE; Liqun; GUO; Xiaojie; LIU; Zhong; LUO; Dawei

    2004-01-01

    The novel differential scanning calorimetry method for determining trapped water volume of human red blood cell during freezing process has been reexamined. Results show that the final erythrocyte volume is 53% of its isotonic volume after freezing to -40℃. An electronic particle counter (MultisizerTM III, Beckman Coulter Inc., USA) was used to measure cell volume changes in response to hypertonic solution. Using this approach, when extracellular solution was 3186 mOsm, the equilibrium cell volume was found to be 57% of its isotonic value. Both results indicate that 34%-40% of intracellular water is trapped and cannot respond to osmotic difference between intra- and extracellular solution. These findings are consistent with the published data: at least 20%-32% of the isotonic cell water volume is retained within RBCs during freezing. Some applications of the values of trapped water are addressed.

  17. Establish and compare the reference intervals of 6 serum enzyme items on the dry and wet chemistry analyzer among healthy children aged from 3 to 6 years in a region%某地区3~6周岁健康儿童干湿化学分析仪6个血清酶项目参考区间的建立与比较研究

    Institute of Scientific and Technical Information of China (English)

    曹科; 马东礼; 罗小娟; 黄宝兴; 吴跃平; 马金香

    2012-01-01

    Objective To establish and compare the reference intervals of 6 serum enzyme items on the dry and wet chemistry analyzer among healthy children aged from 3 to 6 years in Shenzhen,provide better references for clinical diagnosis and treatment. Methods The stratified randomized cluster sampling method was used to select 391 healthy children aged from 3 to 6 years, AST, LDH、ALT、ALP、GGT、CK in venous blood were detected by the VITROS 350 and Beckman Coulter LX20,and data were analyzed by statistical methods. Results The reference intervals showed no significant difference between boys and girls except CK on the dry and Wet chemistry analyzer respectively(P>-0. 05). But the results of 6 serum enzyme items showed significant difference be tween the VITROS 350 and Beckman Coulter LX20(P<0. 05). Conclusion The results showed significant difference between the VITROS 350 and Beckman Coulter LX20,the reference intervals of 6 serum enzyme items on the dry and wet chemistry analyzer a mong healthy children aged from 3 to 6 years in Shenzhen should be established according to different methods.%目的 建立和比较深圳地区3~6周岁健康儿童干湿化学分析仪6个血清酶项目的 参考区间,更好地为临床诊断和治疗服务.方法 采用随机分层抽样筛选出391名健康儿童抽取静脉血,用美国强生Vitros 350干式化学分析仪和美国Beckman Coulter LX20湿化学分析仪进行血清天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、γ-谷氨酰转肽酶(GGT)、肌酸激酶(CK)检测并分析结果.结果 该地区3~6周岁健康儿童两种方法内不同性别血清AST、LDH、ALT、ALP、GGT结果比较,差异均无统计学意义(P>0.05);CK结果差异均有统计学意义(P<0.05);6个血清酶项目两种方法间结果比较,差异均有统计学意义(P<0.05).结论 6个血清酶项目两种方法间结果比较均有显著性差异,应该针对不同方

  18. Access 2日常故障及保养心得

    Institute of Scientific and Technical Information of China (English)

    高昌浩

    2010-01-01

    @@ BECKMAN COULTER Access 2全自动微粒子化学发光免疫分析仪我室已经使用一年半左右,主要用于做甲功五项(促甲状腺激素、游离三碘甲状腺原氨酸、游离甲状腺素、三碘甲状腺原氨酸、甲状腺素)、激素六项(促卵泡激素、促黄体激素、雌二醇、睾酮、泌乳素、孕酮)和人绒毛促性腺激素.此次,就一起长期使用所造成的故障进行讲解.

  19. An easy and reliable automated method to estimate oxidative stress in the clinical setting.

    Science.gov (United States)

    Vassalle, Cristina

    2008-01-01

    During the last few years, reliable and simple tests have been proposed to estimate oxidative stress in vivo. Many of them can be easily adapted to automated analyzers, permitting the simultaneous processing of a large number of samples in a greatly reduced time, avoiding manual sample and reagent handling, and reducing variability sources. In this chapter, description of protocols for the estimation of reactive oxygen metabolites and the antioxidant capacity (respectively the d-ROMs and OXY Adsorbent Test, Diacron, Grosseto, Italy) by using the clinical chemistry analyzer SYNCHRON, CX 9 PRO (Beckman Coulter, Brea, CA, USA) is reported as an example of such an automated procedure that can be applied in the clinical setting. Furthermore, a calculation to compute a global oxidative stress index (Oxidative-INDEX), reflecting both oxidative and antioxidant counterparts, and, therefore, a potentially more powerful parameter, is also described.

  20. 1996 Toxic Hazards Research Annual Report.

    Science.gov (United States)

    1998-01-01

    animals for complete hematology and clinical chemistry assays. Erythrocytes were enumerated on a Coulter counter (Coulter Electronics, Hialeah, FL... complete hematology and clinical chemistry assays. Erythrocytes were enumerated on a Coulter counter (Coulter Electronics, Hialeah, FL) and sera for

  1. POCT法和常规检测法在BNP检测中的对比分析%Comparative analysis of POCT and routine test in BNP testing

    Institute of Scientific and Technical Information of China (English)

    朱小飞; 季明德; 李思洋; 葛亮; 顾万建

    2014-01-01

    Objective To analyze the relevance between the point-of-care testing (POCT) and routine test in BNP testing .Meth-ods The whole blood samples or plasma samples from 40 inpatients were detected brain natriuretic peptide (BNP) by the Alere Triage® MeterPro fluorescence immunoassay analyzer (POCT ) or the Beckman Coulter Access ®2 chemiluminescence analyzer (routine test) ,respectively .The acquired data were subjected to comparative analysis according to the CLCS EP 9-A2 .Results The linear regression of the BNP content in the blood samples detected by POCT and the routine test was good ,the correlation coeffi-cient(r) was 0 .999 7 .Conclusion POCT and the routine test have good correlation in BNP testing .POCT for BNP testing has higher reliability and is applicable for clinical detection .%目的:比较即时检验(POCT )法和常规检测法在B型钠脲肽(BNP)检测中的相关性。方法运用 Alere Triage®MeterPro(美国)荧光免疫分析仪或Beckman Coulter Access®2化学发光仪分别检测40份住院患者全血或血浆样本中BNP含量,按照CLCS EP9-A2文件要求进行对比分析。结果 POCT法和常规检测法检测住院患者血样BNP含量的线性回归良好,相关系数(r)=0.9997。结论 POCT法和常规检测法在BNP检测中的相关性良好,POCT 法具有较高的可靠性,可以用于临床检测。

  2. Complete blood count using VCS (volume, conductivity, light scatter) technology is affected by hyperlipidemia in a child with acute leukemia.

    Science.gov (United States)

    Gokcebay, D G; Azik, F M; Isik, P; Bozkaya, I O; Kara, A; Tavil, E B; Yarali, N; Tunc, B

    2011-12-01

    Asparaginase, an effective drug in the treatment of childhood acute lymphoblastic leukemia (ALL), has become an important component of most childhood ALL regimens during the remission induction or intensification phases of treatment. The incidence range of asparaginase-associated lipid abnormalities that are seen in children is 67-72%. Lipemia causes erroneous results, which uses photometric methods to analyze blood samples. We describe a case of l-asparaginase-associated severe hyperlipidemia with complete blood count abnormalities. Complete blood count analysis was performed with Beckman COULTER(®) GEN·S™ system, which uses the Coulter Volume, Conductivity, Scatter technology to probe hydrodynamically focused cells. Although an expected significant inaccuracy in hemoglobin determination occurred starting from a lipid value of 3450 mg/dl, we observed that triglyceride level was 1466 mg/dl. Complete blood count analysis revealed that exceptionally high hemoglobin, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration levels vs. discordant with red blood cell count, mean corpuscular volume, and hematocrit levels. Total leukocyte count altered spontaneously in a wide range, and was checked with blood smear. Platelet count was in expected range (Table 1). Thus, we thought it was a laboratory error, and the patient's follow-up especially for red cell parameters was made by red blood cell and hematocrit values.

  3. Evaluation of the Sysmex Xe-2100 hematology analyzer in hospital use.

    Science.gov (United States)

    Nakul-Aquaronne, Danièle; Sudaka-Sammarcelli, Isabelle; Ferrero-Vacher, Corinne; Starck, Benoit; Bayle, Jacques

    2003-01-01

    The Sysmex XE-2100 (Sysmex Corp. Kobe, Japan) is a latest-generation hematology analyzer. Its optical and electrical measuring technology is improved by the addition of flux cytometry, fluorescence, and differential lysis. Its analytical performance in terms of precision, reproducibility, linearity, carryover, and time stability was found to be entirely satisfactory. In addition, the results of 500 complete blood counts and differentials correlated perfectly with those obtained by the Coulter STKS (Beckman Coulter, Villapointe, France). The comparison of 500 leukocyte differential count results analyzed in parallel with optical microscopy and the XE-2100 were surprising, and favorable to the XE-2100. This analyzer provides the user with an undeniable feeling of security concerning its reliability in detecting and identifying anomalies in the automated leukocyte differential count. With a sensitivity of 96%, a negative predictive value (NPV) of 98%, and a false-negative (FN) rate of 4%, the XE-2100 has perhaps reached the technological limits for a machine performing morphological recognition of normal and pathological blood cells.

  4. Lymphocytes sensitivity to Fas stimulation in healthy and asthmatic children.

    Directory of Open Access Journals (Sweden)

    Katarzyna Gomułka

    2010-05-01

    Full Text Available The T cell hypothesis of asthma is based on the concept that the disease is driven and maintained by the persistence of a specialized subset of chronically activated T memory cells sensitized against an array of allergenic, occupational or viral antigens. Overreaction of CD4+ T cells in the peripheral blood and airway tissues is an invariant feature of asthma; therefore a potent mechanism for augmenting the number of activated T cells in this disease would be the resistance to the normally programmed pathway for cell death. The aim of the study was to evaluate the presence of apoptotic markers on peripheral blood lymphocytes from healthy and asthmatic children before and after stimulation with antiCD95 antibodies. The blood was collected from 21 children with atopic asthma suffering from allergic rhinitis because of house dust mite and/or grass pollen allergens and 8 healthy children matched for their age and sex. Blood was mixed with purified monoclonal antibody antiCD95 (Beckman Coulter, incubated for 24 hours and than stained with Annexin V andPI (Becton Dickinson. Prepared suspensions were analyzed with Cytomics FC 500 (Beckman Coulter flow cytometer. Annexin V(+/PI(- cells were characterized as early apoptotic, Annexin V(+/PI(+ as late apoptotic and Annexin V(-/PI(+ as dead. In unstimulated sample from asthmatic children 21.09+/-11.20% cells were characterized as Annexin V positive/PI negative. After stimulation with antiCD95 Annexin V positive/PI negative cells constituted 18.72+/-9.42% of cells, p=0.1. In unstimulated sample from healthy children 11.69+/-6.70% cells were characterized as Annexin V positive/PI negative. In the sample stimulated with antiCD95 16.54+/-2.98% of cells were Annexin V positive/PI negative, p=0.02. There were no differences between results of late apoptotic and necrotic lymphocytes from healthy and asthmatic children. Performed research indicates that lymphocytes from asthmatic children are resistant to Fas

  5. Reflotron-Hemoglobin干式血红蛋白检测系统的性能评价%Evaluation on the performance of Reflotron-Hemoglobin dry-chemistry system

    Institute of Scientific and Technical Information of China (English)

    陈骊婷; 王剑飚; 林孝怡; 石厚荣

    2014-01-01

    Objective To evaluate Reflotron-Hemoglobin dry-chemistry system for the measurement of hemoglobin. Methods The within-run and between-run precisions, linearity, correlation and reference range of Reflotron-Hemoglobin dry-chemistry system was verified.Results The within-run precision was 2.54%-2.84%,and the between-run precision was 0.68%-3.38% for Reflotron-Hemoglobin dry-chemistry system,respectively.The linearity range was 52-191 g/L .The results of Reflotron-Hemoglobin dry-chemistry system were correlated well with those of Beckman-Coulter LH750 system,and the correlation equation wan Y=1.031 6X-2.796 9.The verification coincidence rate for reference range was 100%.Conclusions The within-run and between-run precisions of Reflotron-Hemoglobin dry-chemistry system for the determination of hemoglobin are good with accurate results for both normal and abnormal samples.The reference range can be used in this system.%目的:对Reflotron-Hemoglobin干式血红蛋白检测系统进行性能评价。方法对仪器的批内精密度、批间精密度、线性、相关性及参考范围进行验证。结果 Reflotron-Hemoglobin干式血红蛋白检测系统的批内精密度为2.54%~2.84%;批间精密度为0.68%~3.38%;线性范围为52~191 g/L;与 BECKMAN-COULTER LH750全自动血液分析仪的相关方程为 Y=1.0316X-2.7969;参考区间验证符合率为100%。结论Reflotron-Hemoglobin 干式血红蛋白检测系统批内、批间精密度较好;在正常及病理范围内检测结果可靠;常用参考区间适用于本仪器。

  6. Improved differential diagnosis of anemia of chronic disease and iron deficiency anemia: a prospective multicenter evaluation of soluble transferrin receptor and the sTfR/log ferritin index.

    Science.gov (United States)

    Skikne, Barry S; Punnonen, Kari; Caldron, Paul H; Bennett, Michael T; Rehu, Mari; Gasior, Gail H; Chamberlin, Janna S; Sullivan, Linda A; Bray, Kurtis R; Southwick, Paula C

    2011-11-01

    Anemia of chronic disease (ACD) and iron deficiency anemia (IDA) are the most prevalent forms of anemia and often occur concurrently. Standard tests of iron status used in differential diagnosis are affected by inflammation, hindering clinical interpretation. In contrast, soluble transferrin receptor (sTfR) indicates iron deficiency and is unaffected by inflammation. Objectives of this prospective multicenter clinical trial were to evaluate and compare the diagnostic accuracy of sTfR and the sTfR/log ferritin index (sTfR Index) for differential diagnosis using the automated Access(®) sTfR assay (Beckman Coulter) and sTfR Index. We consecutively enrolled 145 anemic patients with common disorders associated with IDA and ACD. Subjects with IDA or ACD + IDA had significantly higher sTfR and sTfR Index values than subjects with ACD (P Index: 14 (using nmol/L) (or 1.03 using mg/L). The sTfR Index was superior to sTfR (AUC 0.87 vs. 0.74, P Index). Use of sTfR and the sTfR Index improves detection of IDA, particularly in situations where routine markers provide equivocal results. Findings demonstrate a significant advantage in the simultaneous determination of ferritin, sTfR and sTfR Index. Obtaining a ferritin level alone may delay diagnosis of combined IDA and ACD.

  7. Mean reticulocyte volume enhances the utility of red cell mean sphered cell volume in differentiating peripheral blood spherocytes of hereditary spherocytosis from other causes

    Directory of Open Access Journals (Sweden)

    Sukesh C Nair

    2015-01-01

    Full Text Available Context: Mean sphered cell volume (MSCV and mean reticulocyte volume (MRV are additional reticulocyte parameters generated while processing the blood samples on Beckman coulter LH 755 in the reticulocyte mode using the volume, conductivity and scatter technology. It has been observed that the difference between mean corpuscular volume (MCV and MSCV is higher in the cases of hereditary spherocytosis (HS and this difference is increasingly being utilized as a screening tool for spherocytes. In addition now there have been new observations that reticulocyte volume in cases of HS is less as compared to normal reticulocyte. Aims: Our aim was to test the usefulness of reticulocyte parameters like MSCV and MRV in distinguishing cases of HS and autoimmune hemolytic anemia (AIHA. Materials and Methods: This is a retrospective and partly prospective study where peripheral blood ethylenediaminetetraacetic acid samples from cases of HS (n = 57 and AIHA (n = 29 were processed on LH 755 in both the differential and the reticulocyte mode. The data generated were analyzed and compared with data from normal healthy donors (n = 46. Results: Using an algorithm of MCV - MSCV >10 and MRV - MSCV <25, a sensitivity of 84.2% and specificity of 94.7% was observed in cases of HS. Conclusions: With the reticulocyte analysis, we may now have a simple and cheap additional tool for screening of HS.

  8. Automated reticulocyte parameters for hereditary spherocytosis screening.

    Science.gov (United States)

    Lazarova, Elena; Pradier, Olivier; Cotton, Frédéric; Gulbis, Béatrice

    2014-11-01

    The laboratory diagnosis of hereditary spherocytosis (HS) is based on several screening and confirmatory tests; our algorithm includes clinical features, red blood cell morphology analysis and cryohaemolysis test, and, in case of positive screening, sodium dodecyl sulphate polyacrylamide gel electrophoresis as a diagnostic test. Using the UniCel DxH800 (Beckman Coulter) haematology analyser, we investigated automated reticulocyte parameters as HS screening tool, i.e. mean reticulocyte volume (MRV), immature reticulocyte fraction (IRF) and mean sphered cell volume (MSCV). A total of 410 samples were screened. Gel electrophoresis was applied to 159 samples that were positive for the screening tests. A total of 48 patients were diagnosed as HS, and seven were diagnosed as acquired autoimmune haemolytic anaemia (AIHA). Some other 31 anaemic conditions were also studied. From the receiver operating characteristic (ROC) curve analysis, both delta (mean cell volume (MCV)-MSCV) and MRV presented an area under the curve (AUC) of 0.98. At the diagnostic cut-off of 100 % sensitivity, MRV showed the best specificity of 88 % and a positive likelihood ratio of 8.7. The parameters IRF, MRV and MSCV discriminated HS not only from controls and other tested pathologies but also from AIHA contrary to the cryohaemolysis test. In conclusion, automated reticulocyte parameters might be helpful for haemolytic anaemia diagnostic orientation even for general laboratories. In combination with cryohaemolysis, they ensure an effective and time-saving screening for HS for more specialised laboratories.

  9. An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems

    Directory of Open Access Journals (Sweden)

    Verschraegen Gerda

    2005-03-01

    Full Text Available Abstract Background Currently, most laboratories identify yeasts routinely on the basis of morphology and biochemical reactivity. This approach has quite often limited discriminatory power and may require long incubation periods. Due to the increase of fungal infections and due to specific antifungal resistence patterns for different species, accurate and rapid identification has become more important. Several molecular techniques have been described for fast and reliable identification of yeast isolates, but interlaboratory exchangeability of identification schemes of molecular techniques has hardly been studied. Here, we compared amplified ITS2 fragment length determination by an ABI Prism 310 (Applied Biosystems, Foster City, Ca. capillary electrophoresis system with that obtained by a CEQ8000 (Beckman Coulter, Fullerton, Ca. capillary electrophoresis system. Results Although ITS2 size estimations on both systems differed and separate libraries had to be constructed for each system, both approaches had the same discriminatory power with regard to the 44 reference strains, identical identifications were obtained for 39/ 40 clinical isolates in both laboratories and strains from 51 samples were correctly identified using CEQ8000, when compared to phenotypic identification. Conclusion Identification of yeasts with ITS2-PCR followed by fragment analysis can be carried out on different capillary electrophoresis systems with comparable discriminatory power.

  10. Normal lymphocyte immunophenotype in an elderly population

    Directory of Open Access Journals (Sweden)

    Sâmia Macedo Queiroz Mota Castellão Tavares

    2014-06-01

    Full Text Available OBJECTIVE: The aim of this work was to evaluate the lymphocyte immunophenotype in an elderly population.METHODS: This study enrolled 35 over 60-year-old volunteers and a control group composed of 35 young adults. The study included elderly without diseases that might affect the functioning of the immune system. These individuals were consulted by doctors and after a physical examination, laboratory tests were performed using a Beckman Coulter (r flow cytometer. The GraphPad Prism computer program was employed for statistical analysis with the level of significance being set for p-values <0.05.RESULTS: There is a statistically significant reduction in the number of lymphocytes (CD8 +, CD2 + and CD3 + cells in the elderly compared to young adults. These low rates are explained by changes attributed to aging and may be partly responsible for the reduction in the cellular immune response, lower proliferative activity and the low cytotoxicity of lymphocytes.CONCLUSION: These parameters showed greater impairment of adaptive immunity in the elderly population and can therefore explain the greater fragility of the aged body to developing diseases.

  11. Apoptosis induced by (di-isopropyloxyphoryl-Trp)2-Lys-OCH3 in K562 and HeLa cells

    Indian Academy of Sciences (India)

    Feng Liu; Shi-Ying Liu; Ping Xu; Zhen-Hua Xie; Guo-Ping Cai; Yu-Yang Jiang

    2008-03-01

    According to the method used in our laboratory, our group synthesized (DIPP-Trp)2-Lys-OCH3. It inhibited the proliferation of K562 and HeLa cells in a dose- and time-dependent manner with an IC50 of 15.12 and 42.23 M, respectively. (DIPP-Trp)2-Lys-OCH3 induced a dose-dependent increase of the G2/M cell population in K562 cells, and S cell population in HeLa cells; the sub-G0 population increased dramatically in both cell lines as seen by PI staining experiments using a FACS Calibur Flow cytometer (BeckmanCoulter, USA). Phosphatidylserine could significantly translocate to the surface of the membrane in (DIPP-Trp)2-Lys-OCH3-treated K562 and HeLa cells. The increase of an early apoptotic population was observed in a dose-dependent manner by both annexin-FITC and PI staining. It was concluded that (DIPP-Trp)2-Lys-OCH3 not only induced cells to enter into apoptosis, but also affected the progress of the cell cycle. It may have arrested the K562 and HeLa cells in the G2/M, S phases, respectively. The apoptotic pathway was pulsed at this point, resulting in the treated cells entering into programmed cell death. (DIPP-Trp)2-Lys-OCH3 is a potential anticancer drug that intervenes in the signalling pathway.

  12. Updates in immunoassays: virology.

    Science.gov (United States)

    Josko, Deborah

    2012-01-01

    Virus identification is a challenge to the clinical microbiologist since growing viruses in traditional cell culture is labor intensive, time consuming, and subject to contamination. The advent of rapid and automated immunoassays has eliminated this problem by generating positive results in minutes to hours. For example, testing for infectious mononucleosis can yield a positive result in 3-8 minutes as seen with the Beckman Coulter, Inc. ICON Mono test or in 5-15 minutes with the MONO Mononucleosis Rapid Test Device marketed by ACON Laboratories, Inc. Fully automated immunoassay analyzers provide fast, accurate, sensitive results that aid in a prompt and accurate diagnosis for the patient. Turnaround times are shortened, allowing for timely medical intervention and treatment. The priority in any hospital or medical facility is to treat the patient as quickly and appropriately as possible. By using immunoassays, clinical laboratory professionals are able to report out correct results in a timely manner, ensuring overall positive patient outcomes and improved quality of healthcare.

  13. Ultrasonic sonoelastography of scrotum in the diagnosis of male fertility

    Directory of Open Access Journals (Sweden)

    O. B. Zhukov

    2014-01-01

    Full Text Available Objective: to determine the possibility of using detect the scrotum in assessing reproductive function of men.Materials and methods. The study was conducted in men aged 20–44 years. The first group of men was 25 patients with secretory infertility. The second group included 25 male infertility varicocele. The control group presents 12 men in the age range 22–31 year with the implementation of the reproductive function of 1 or more children. The study was performed by ultrasonic scanner expert class Acuson S2000 manufacture of the company Siemens, was used microwave sensor 18L6 HD. Sex hormones were studied on immunochemiluminescence analyzer Access 2 production Beckman Coulter (USA. Obtaining and study of ejaculate was carried out according to Recommendations of the WHO (5th edition.Results. The application of research shear transverse wave Vs men with secretory infertility and varicocele is accompanied by the increase of its value to 1.5–1.6 m/s and is characterized by 35–45 % from similar parameters of reproductive healthy men. In half of the cases studies such an increase in performance and decrease in the area of mapping the so-called “soft” areas of the epididymis is accompanied by a decrease in the concentration and actively motile sperm. The use of such method of instrumental diagnostics will allow at the stage of preliminary ultrasound screening to suspect a violation of the reproductive capacity of men.

  14. Dichloromethane-methanol extract from Borassus aethiopumn mart. (Arecaceae) induces apoptosis of human colon cancer HT-29 cells.

    Science.gov (United States)

    Sakandé, J; Rouet-benzineb, P; Devaud, H; Nikiema, J B; Lompo, M; Nacoulma, O G; Guissou, I P; Bado, A

    2011-05-15

    Borassus aetihiopum MART (Arecaceae) is a plant used in traditional herbal medicine for the treatment of various diseases (bronchitis, laryngitis, antiseptic). In particular, their male inflorcscences were reported to exhibit cicatrizing, antiseptic and fungicidal properties. In the present study, the biological activity of E2F2, an apolar extract from Borassus aethiopum male inflorescence was investigated on colon cancer HT29 cells. Phytochemical screening was carried according to methodology for chemical analysis for vegetable drugs. Cells proliferation was determined by the MTT assay and cells cycle distribution was analysed by using laser flow cytometer (Beckman coulter). The cytoskeleton organisation was examined under a laser scanning confocal microscope (Zess). Preliminary phytochemical analysis of E2F2 extract revealed the presence of sterols, triterpenes and saponosids. E2F2 extract (1 microg and 100 microg mL(-1)) significantly inhibited cell proliferation by blocking cell population in G0/G1 phase. Flow Cytometric analysis of E2F2-treated HT29 cells showed that hypoploïd cell population (sub G1 phase) increased with processing time exposures. Immunofluorescence confocal analysis revealed a disrupt actin microfilaments network in E2F2 treated-cells with a significant reduction in actin stress fibres and appearance of a random, non-oriented distribution of focal adhesion sites. These data indicate that E2F2 extract has anti-proliferative and pro-apoptotic activities. Further studies are required to unravel the mechanisms of action of E2F2 extract.

  15. Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis.

    Science.gov (United States)

    Torres-Morales, Elizabeth; Taborda, Laura; Cardona, Nestor; De-la-Torre, Alejandra; Sepulveda-Arias, Juan Carlos; Patarroyo, Manuel Alfonso; Gomez-Marin, Jorge Enrique

    2014-10-01

    We determined the specific lymphocyte proliferative response and cytokine profile production regarding Toxoplasma P30 (2017 from virulent and non-virulent strain) and ROP18 protein-derived peptides (from clonal lineages I, II and III) in 19 patients having ocular toxoplasmosis, five suffering chronic asymptomatic infection, nine with congenital toxoplasmosis and eight Toxoplasma negative people. A Beckman Coulter FC500 flow cytometer was used for determining antigen-specific T cells (CD3+ CD4+ or CD3+ CD8+ cells) in peripheral blood culture. IFN γ and IL10 levels were determined in culture supernatants. Specific CD4+ and CD8+ T cell response to total antigen and P30- and ROP18-derived peptides was observed in infected people. Ocular toxoplasmosis patients had a preferential Th2 response after antigenic stimulation. Non-virulent peptide 2017 was able to shift response toward Th1 in congenitally infected children and virulent peptide 2017 induced a Th2 response in chronically infected, asymptomatic people. An immune response in human toxoplasmosis after ex vivo antigenic stimulation was Th1- or Th2-skewed, depending on a patient's clinical condition. Colombian ocular toxoplasmosis patients' immune response was Th2-skewed, regardless of the nature of antigen stimulus.

  16. Ultrasonic sonoelastography of scrotum in the diagnosis of male fertility

    Directory of Open Access Journals (Sweden)

    O. B. Zhukov

    2014-12-01

    Full Text Available Objective: to determine the possibility of using detect the scrotum in assessing reproductive function of men.Materials and methods. The study was conducted in men aged 20–44 years. The first group of men was 25 patients with secretory infertility. The second group included 25 male infertility varicocele. The control group presents 12 men in the age range 22–31 year with the implementation of the reproductive function of 1 or more children. The study was performed by ultrasonic scanner expert class Acuson S2000 manufacture of the company Siemens, was used microwave sensor 18L6 HD. Sex hormones were studied on immunochemiluminescence analyzer Access 2 production Beckman Coulter (USA. Obtaining and study of ejaculate was carried out according to Recommendations of the WHO (5th edition.Results. The application of research shear transverse wave Vs men with secretory infertility and varicocele is accompanied by the increase of its value to 1.5–1.6 m/s and is characterized by 35–45 % from similar parameters of reproductive healthy men. In half of the cases studies such an increase in performance and decrease in the area of mapping the so-called “soft” areas of the epididymis is accompanied by a decrease in the concentration and actively motile sperm. The use of such method of instrumental diagnostics will allow at the stage of preliminary ultrasound screening to suspect a violation of the reproductive capacity of men.

  17. Journal of Computational Electronics: Proceedings of the International Workshop on Computational Electronics (8th) (IWCE-8), Beckman Institute, University of Illinois, 15-18 October 2001. Volume 1, Issue 1-2

    Science.gov (United States)

    2002-01-01

    other parallel cod- NASA/JPL Applications. AIAA Space 2001 Conference and Ex- Decyk fposition, Albuquerque, New Mexico . ing issues, as well as Frank...were implemented in the 2-D - Schr~dinger-Polsson full-band Monte Carlo simulator, MOCA (Duncan, 1 Schradinger--oected MC10• 3’.0’ I Wi ner-based MC...resulting current from the transfer significant way. A self-consistent simulation with the matrix and Monte Carlo methods. All of the quantum full-band MOCA

  18. Changes in Soldier Nutritional Status and Immune Function During the Ranger Training Course

    Science.gov (United States)

    1992-09-10

    Synchron CX5 automated chemistry analyzer (Beckman Instruments, Fullerton, CA) with manufacturer recommended reagents. Test Method Wavelenath Glucose...plasma samples with EDTA as the anticoagulant using the Beckman Synchron CX5 automated chemistry analyzer with manufacturer recommended assay systems

  19. A new database sub-system for grain-size analysis

    Science.gov (United States)

    Suckow, Axel

    2013-04-01

    Detailed grain-size analyses of large depth profiles for palaeoclimate studies create large amounts of data. For instance (Novothny et al., 2011) presented a depth profile of grain-size analyses with 2 cm resolution and a total depth of more than 15 m, where each sample was measured with 5 repetitions on a Beckman Coulter LS13320 with 116 channels. This adds up to a total of more than four million numbers. Such amounts of data are not easily post-processed by spreadsheets or standard software; also MS Access databases would face serious performance problems. The poster describes a database sub-system dedicated to grain-size analyses. It expands the LabData database and laboratory management system published by Suckow and Dumke (2001). This compatibility with a very flexible database system provides ease to import the grain-size data, as well as the overall infrastructure of also storing geographic context and the ability to organize content like comprising several samples into one set or project. It also allows easy export and direct plot generation of final data in MS Excel. The sub-system allows automated import of raw data from the Beckman Coulter LS13320 Laser Diffraction Particle Size Analyzer. During post processing MS Excel is used as a data display, but no number crunching is implemented in Excel. Raw grain size spectra can be exported and controlled as Number- Surface- and Volume-fractions, while single spectra can be locked for further post-processing. From the spectra the usual statistical values (i.e. mean, median) can be computed as well as fractions larger than a grain size, smaller than a grain size, fractions between any two grain sizes or any ratio of such values. These deduced values can be easily exported into Excel for one or more depth profiles. However, such a reprocessing for large amounts of data also allows new display possibilities: normally depth profiles of grain-size data are displayed only with summarized parameters like the clay

  20. Comparison analysis between different biochemical analysis systems%三台不同生化分析仪间部分生化项目结果比对和偏倚评估

    Institute of Scientific and Technical Information of China (English)

    梁紫甄; 赵莹; 陈顺仪; 黄艳佳; 罗颖

    2016-01-01

    目的:分析日本Olympus AU5400型全自动生化分析仪(简称AU5400)与美国Beckman Coulter AU5800型全自动生化分析仪(简称AU5800)、美国强生Vitros FS 5,1全自动干生化分析仪(简称FS 5,1)的葡萄糖(Glu)、钾(K+)、钙(Ca)、肌酐(Cr)四个项目检测结果的可比性。方法按美国国家临床实验室标准化委员会(NCCLS) EP9-A2文件,以AU5400为参考系统,AU5800和FS 5,1为试验系统,同时检测新鲜血清标本的Glu、K+、Ca、Cr,计算相关系数(r)和回归方程,并以1/2 CLIA'88允许偏差范围为标准,对临床可接受性进行分析。结果所有检测项目在试验系统与参考系统间的检测结果的相关系数均大于0.975;除Cr外,其他三项在医学决定水平处的预期偏倚都在临床可接受范围内。结论三套分析系统具有良好的相关性,Glu、K+、Ca三个项目的检测结果具有可比性,可相互认可并被临床所接受;Cr则对其在系统间的不可比性进行原因分析,并在校正后重新分析偏移评估和相关性,也在临床可接受范围内。%Objective To investigate the comparability of Glu, K+, Ca and Cr measurement between Olympus AU5400 analyzer, Beckman Coulter AU5800 and Vitros FS 5,1 analyzer. Methods According to the Ep9-A2 docu-ment of the Clinical and Laboratory Standards Institute, AU5400 analyzer was used as the reference system, and AU5800 and FS 5,1 analyzer were selected as the experimental system. Meanwhile, the levels of Glu, K+, Ca and Cr of serum were tested. The correlation coefficient and regression equation were calculated and the clinical acceptability was calculated according to the range of the half of the allowed total error of CLIA'88. Results There were significant cor-relation between the reference system and the experimental system in all the tested projects (r>0.975);the expected bias of the other three could be accepted in clinical acceptable range except Cr

  1. 复凝聚法制备桃金娘油肠溶微囊%Enteric myrtle oil microcapsules by complex coacervation

    Institute of Scientific and Technical Information of China (English)

    蔡翠芳; 曾雪萍; 何海冰; 齐娜; 唐星

    2011-01-01

    Objective To prepare myrtle oil enteric-coated microcapsules using a complex coacervation method and evaluate the in vitro performance. Methods Sodium alginate,calcium chloride and chitosan were selected as the materials for myrtle oil loaded microcapsules; surface morphology and size of microcapsules were characterized by scanning electronic microscopy (SEM) and laser diffraction particle size analyzer (Beckman Coulter LS 230). The headspace gas chromatography (HS-GC) method was employed to determine the loading capacity and encapsulation efficiency. Results Using orthogonal design, formulation and process were optimized as follows :sodium alginate concentration was 25 g·L-1, chitosan concentration was 3 g ·L-1,condensation rate was 5 mL·min-1 and time was 60 min. The drug loaded enteric microcapsules showed shrunken surface,narrow size distribution with average particle size of( 14. 23 ± 1.45) μm,loading capacity of(11. 3 ±0.4)% , and good acid resistance. Conclusions Optimized myrtle oil loaded microcapsules demonstrates higher dose, enteric features and good reproducibility. This method can be used for the preparation of myrtle oil enteric microcapsules.%目的 采用复凝聚法制备桃金娘油肠溶微囊,并对其体外性质进行评价.方法 选用海藻酸钠、氯化钙、壳聚糖为囊材采用复凝聚法制备桃金娘油微囊,用扫描电子显微镜(scanning electron microscope,SEM),Beckman Coulter LS 230激光粒度仪表征了微囊表面形态及粒径,采用顶空进样-GC色谱法测定了载药量和包封率.结果 正交设计优化处方和工艺如下:海藻酸钠质量浓度为25g·L-1、壳聚糖质量浓度为3 g·L-1、凝聚速度为5 mL·min-1和凝聚时间为60min,所得微囊粒径为(14.23±1.45)μm,载药质量分数为(11.3±0.4)%,包封率为(73.6±2.5)%.微囊具有 耐酸和肠溶性能,表面褶皱,粒径分布均匀.结论 复凝聚法可用于桃金娘油肠溶微囊的制备.

  2. 同型半胱氨酸和叶酸、维生素B12联合检测在心血管疾病中的作用%Combinative Function of Hcy,FA and VitB12 in Analysis of Cardiovascular Disease

    Institute of Scientific and Technical Information of China (English)

    孟岩; 王蕾; 崔桂华; 郝彦平; 刘辉; 靳松

    2011-01-01

    Objective To study the relationship between cardiovascular disease and serum,homocysteine (Hcy),folic acid (FA) ,and the density of VitB12. Methods To determine the relativity between Hcy and the level of FA,VitB12.120 cases were selected after coronary arteriography. Enzymatic cycling assay was introduced to test the level of serum Hcy by using an apparatus,Hitachi 7170,automatic biochemical analyzer. Automated microparticle chemiluminescence immunoassay ahalyzer (Beckman-Coulter ACCESS) was used to test the level of FA,VitB12. Results ① The level of Hcy in group with coronary disease was higher than those in control group(P<0. 0l). ② Patients with coronary artery disease Hcy level and serum FA and VitB12 was a negative correlation(r =- 0. 64;r =- 0. 44,P<0.01). ③Patients with coronary artery disease by taking FA,VitB12 and VitB6 after treatment serum Hcy concentration obvious(r =- 0. 64;r =- 0. 44,P<0. 0l). Conclusion The high level of Hcy is a higher risk of independent factor with coronary disease.%目的 探讨血清同型半胱氨酸(Hcy)、叶酸(FA)、维生素B12(Vit B12)浓度与心血管病的关系.方法 选择经冠状动脉造影检查的患者共120例,血清同型半胱氨酸(Hcy)采用日立7170全自动生化分析仪,方法为循环酶法;叶酸(FA)、维生素B12(Vit B12)采用Beckman-Coulter ACCESS全自动微粒子化学发光免疫分析仪检测.观察Hcy与FA,VitB12水平变化的相关性.结果 ①冠心病患者血清Hcy含量显著高于对照组(P<0.01).②冠心病患者血清Hcy水平与FA,VitB12呈负相关(r=-0.64;r=-0.44,P<0.01).③冠心病患者经服用叶酸(FA)、维生素B12(VitB12)、维生素B6(VB6)治疗后,血清Hcy浓度降低明显(P<0.01).结论 高同型半胱氨酸水平是冠心病的独立危险因素.

  3. Study on Profiling Mitochondria Proteome of Human Fetal Liver by Multi-dimensional Liquid Chromatography and Liquid Chromatography-Ion Trap Mass Spectrometry%多维液相色谱及液相色谱-离子阱质谱法研究人胎肝线粒体蛋白质组

    Institute of Scientific and Technical Information of China (English)

    张养军; 石蓉; 孟庆芳; 王京兰; 蔡耘; 朱云平; 贺福初; 钱小红

    2004-01-01

    In proteomics study, because there were biases of two-dimensional gel electrophoresis in molecular weights, pH and hydrophobicity, multi-dimensional protein identification technology (MuD-PIT)was proposed. Due to the core part of the technique being digestion of total proteins in the first step, some drawbacks appeared, such as complicating the separation of samples, dominating the influence of proteins with high abundance on the identification of proteins with low abundance and losing some information of proteins,for example, post-translational modification et al. , these resulted in the limitation of applications for the method. Taking into account of those factors mentioned above, when studying the mitochondria proteome of human fetal liver with great significance in life process, Proteome Lab PF2D system (Beckman Coulter, USA) was employed. The fractions of extract from fetal liver tissues by saccharose- density gradient centrifugation were prepared on a high velocity centrifuge. The desalting and solvent exchanging of the fractions with Start buffer (pH8. 5, Beckman Coulter, USA) were carried out on a PD-10(Sephadex G-25, 5 000Da) desalting column (amersham pharmacia biotech, Wikstroms,Sweden) according to the procedures described in its product instruction. Then the treated fractions were separated with chromatofocusing and reversed-phase liquid chromatography in tandem, respectively. After concentration and in-solution digestion of protein fractions,the peptide mixture was lyophilized, then resolved with 5% aqueous acytonitrile plus 0.1% formic acid, and finally analyzed with microLC-ESI-ion trap mass spectrometry(Thermo Finnigan, San Jose, CA, USA) and CapLC-ESI-QTOF mass spectrometry (Waters, USA). Mass spectrometric data was searched against IPI protein database with Thermo SEQUEST (Thermo Finnigan, San Jose, CA, USA) and Mascot. A primary database of mitochondrial proteome of human fetal liver was constructed, which could play central role in the large

  4. 多维液相色谱及液相色谱-离子阱质谱法研究人胎肝线粒体蛋白质组

    Institute of Scientific and Technical Information of China (English)

    张养军; 石蓉; 孟庆芳; 王京兰; 蔡耘; 朱云平; 贺福初; 钱小红

    2004-01-01

    In proteomics study, because there were biases of two-dimensional gel electrophoresis in molecular weights, pH and hydrophobicity, multi-dimensional protein identification technology (MuD-PIT)was proposed. Due to the core part of the technique being digestion of total proteins in the first step, some drawbacks appeared, such as complicating the separation of samples, dominating the influence of proteins with high abundance on the identification of proteins with low abundance and losing some information of proteins,for example, post-translational modification et al. , these resulted in the limitation of applications for the method. Taking into account of those factors mentioned above, when studying the mitochondria proteome of human fetal liver with great significance in life process, Proteome Lab PF2D system (Beckman Coulter, USA) was employed. The fractions of extract from fetal liver tissues by saccharose- density gradient centrifugation were prepared on a high velocity centrifuge. The desalting and solvent exchanging of the fractions with Start buffer (pH8. 5, Beckman Coulter, USA) were carried out on a PD-10(Sephadex G-25, 5 000Da) desalting column (amersham pharmacia biotech, Wikstroms,Sweden) according to the procedures described in its product instruction. Then the treated fractions were separated with chromatofocusing and reversed-phase liquid chromatography in tandem, respectively. After concentration and in-solution digestion of protein fractions,the peptide mixture was lyophilized, then resolved with 5% aqueous acytonitrile plus 0.1% formic acid, and finally analyzed with microLC-ESI-ion trap mass spectrometry(Thermo Finnigan, San Jose, CA, USA) and CapLC-ESI-QTOF mass spectrometry (Waters, USA). Mass spectrometric data was searched against IPI protein database with Thermo SEQUEST (Thermo Finnigan, San Jose, CA, USA) and Mascot. A primary database of mitochondrial proteome of human fetal liver was constructed, which could play central role in the large

  5. Proteomic methodological recommendations for studies involving human plasma, platelets, and peripheral blood mononuclear cells.

    Science.gov (United States)

    de Roos, Baukje; Duthie, Susan J; Polley, Abigael C J; Mulholland, Francis; Bouwman, Freek G; Heim, Carolin; Rucklidge, Garry J; Johnson, Ian T; Mariman, Edwin C; Daniel, Hannelore; Elliott, Ruan M

    2008-06-01

    This study was designed to develop, optimize and validate protocols for blood processing prior to proteomic analysis of plasma, platelets and peripheral blood mononuclear cells (PBMC) and to determine analytical variation of a single sample of depleted plasma, platelet and PBMC proteins within and between four laboratories each using their own standard operating protocols for 2D gel electrophoresis. Plasma depleted either using the Beckman Coulter IgY-12 proteome partitioning kit or the Amersham albumin and IgG depletion columns gave good quality gels, but reproducibility appeared better with the single-use immuno-affinity column. The use of the Millipore Filter Device for protein concentration gave a 16% ( p appears as a single abundant spot. The average within-laboratory coefficient of variation (CV) for each of the matched spots after automatic matching using either PDQuest or ProteomWeaver software ranged between 18 and 69% for depleted plasma proteins, between 21 and 55% for platelet proteins, and between 22 and 38% for PBMC proteins. Subsequent manual matching improved the CV with on average between 1 and 16%. The average between laboratory CV for each of the matched spots after automatic matching ranged between 4 and 54% for depleted plasma proteins, between 5 and 60% for platelet proteins, and between 18 and 70% for PBMC proteins. This variation must be considered when designing sufficiently powered studies that use proteomics tools for biomarker discovery. The use of tricine in the running buffer for the second dimension appears to enhance the resolution of proteins especially in the high molecular weight range.

  6. Detection of an IncA/C plasmid encoding VIM-4 and CMY-4 β-lactamases in Klebsiella oxytoca and Citrobacter koseri from an inpatient in a cardiac rehabilitation unit.

    Science.gov (United States)

    Caltagirone, Mariasofia; Bitar, Ibrahim; Piazza, Aurora; Spalla, Melissa; Nucleo, Elisabetta; Navarra, Antonella; Migliavacca, Roberta

    2015-07-01

    A 62-year-old patient was transferred to the cardiac rehabilitation unit of the I.R.C.C.S. Fondazione S. Maugeri after undergoing a heart transplantation at the Acute Care Hospital I.R.C.C.S. S. Matteo of Pavia. On 1 August 2013 and during hospitalization in the rehabilitation unit, Klebsiella oxytoca and Citrobacter koseri clinical isolates were simultaneously recovered from the patient's preputial swab. Both the K. oxytoca and C. koseri strains were carbapenem- resistant by MicroScan System (Beckman Coulter). Carbapenem-resistant K. pneumoniae had previously been reported in the same rehabilitation facility. The aim of the study was to identify the carbapenem resistance mechanisms among the enterobacterial species recovered. Phenotypic screening tests useful to detect the β-lactamases/carbapenemases were performed. Carbapenem MICs were obtained by Etest. AmpC and MBL encoding genes were identified by PCR and sequencing. Conjugation assays and plasmid characterization were performed. Both of the K. oxytoca and C. koseri isolates were multi drug resistant, showing resistance to amoxicillin-clavulanic acid, three generation cephalosporins, ertapenem (K. oxytoca MIC, >32 mg/L; C. koseri MIC, 4 mg/L), imipenem (K. oxytoca MIC, 4 mg/L; C. koseri MIC, 12 mg/L), thrimethoprim sulphamethoxazole and gentamicin. Susceptibility was retained to fluoroquinolones, colistin and tigecycline. Molecular characterization confirmed the co-presence of blaCMY-4 and blaVIM-4 determinants in a 150 Kb transferable plasmid of IncA/C group. This case is the first detection in Italy of the K. oxytoca and C. koseri clinical isolates co-producing the CMY-4 and VIM-4 enzymes.

  7. The Effects of Short-Term Intensive Exercise on Levels of Liver Enzymes and Serum Lipids in Kick Boxing Athletes

    Directory of Open Access Journals (Sweden)

    Ömer Kaynar

    2016-03-01

    Full Text Available Objective: In this study, it was aimed to evaluate the ef­fects of short-term intensive exercise on liver enzymes and serum lipid levels with kick boxing athletes. Methods: 23 voluntary athletes who were between the ages of 15-46 and who engaged in kick–boxing have tak­en place this study. Athletes were made to do 45 minutes of warming-up, breathing, and stretching and 50 minutes of technical and tactical practices and then they were made to do a training match, which is equal to a 2 min­utes 3 circuits (1 minute rest kick-box match. In venous blood samples which were taken from athletes before and after training, aspartate aminotransferase (AST, alanine aminotransferase (ALT, alkaline phosphatase (ALP and gamma glutamine transpeptidase (GGT, enzyme activity and total cholesterol, high-density lipoprotein-cholesterol (HDL-C, low density lipoprotein-cholesterol (LDL-C and triglycerides serum levels were analyzed via spectropho­tometric method in Beckman Coulter AU 5800 auto ana­lyzer. Body composition measurements of athletes were made with Tanita TBF 300 brand device, which works with bio-impedance analysis (BIA system. Results: As a result of our study, statistically increases in serum ALT, AST, ALP and GGT enzyme activities and in serum total cholesterol, HDL-C and LDL-C levels were detected following short-term intensive exercise, but no significant difference was observed in TG levels after in­tensive exercise. Conclusion: The blows to the abdomen during kickbox­ing sports competitions result in increased liver enzymes and increased serum lipids may occur to meet energy de­mand of the body during exercise.

  8. A technique for dating toxoplasmosis in pregnancy and comparison with the Vidas anti-toxoplasma IgG avidity test.

    Science.gov (United States)

    Flori, P; Bellete, B; Crampe, C; Maudry, A; Patural, H; Chauleur, C; Hafid, J; Raberin, H; Tran Manh Sung, R

    2008-03-01

    A comparative evaluation of 384 selected sera was performed using the Beckman Coulter Access and Abbott Axsym Toxo-IgG assays. The Axsym assay yields positive early results following infection, while the Access assay gives higher titres during chronic infection. The ratio between the two complementary tests, Axsym Toxo-IgG/Access Toxo-IgG (Ax/Ac), was compared with the Vidas anti-Toxoplasma IgG avidity index (AI). The Ax/Ac ratio decreased progressively as the time between infection and sampling increased. The mean Ax/Ac values (+/-SE) were 2.50 (+/-0.26), 2.14 (+/-0.13), 2.33 (+/-0.22), 1.34 (+/-0.09), 1.32 (+/-0.10), 0.92 (+/-0.08) and 0.74 (+/-0.07) for groups of sera sampled at 1, 2, 3, 4-5, 6-8, 9-12 and 13-24 months, respectively, after infection in pregnant women. These values were much smaller for cases with chronic infection (>24 months), i.e., 0.56 (+/-0.03), 0.44 (+/-0.04) and 0.53 (+/-0.04), respectively, for pregnant women and immunodepressed patients with and without reactivation. Taking a ratio of 1 as a threshold for recent infection, the patients in the groups sampled at 1, 2 and 3 months had Ax/Ac ratios >1 in 49/50 (98%), 53/55 (96.4%) and 36/36 (100%) cases, respectively. Thus, an Ax/Ac ratio of <1 in serum from a pregnant woman allows a recent infection (<3 months) to be excluded. This technique has the advantage of yielding positive results that develop much more rapidly than the AI, thereby helping to reassure large numbers of pregnant women and avoiding costly and unnecessary prophylactic treatment and follow-up.

  9. Mean corpuscular volume of control red blood cells determines the interpretation of eosin-5'-maleimide (EMA) test result in infants aged less than 6 months.

    Science.gov (United States)

    Ciepiela, Olga; Adamowicz-Salach, Anna; Bystrzycka, Weronika; Łukasik, Jan; Kotuła, Iwona

    2015-08-01

    Eosin-5'-maleimide (EMA) binding test is a flow cytometric test used to detect hereditary spherocytosis (HS). To perform the test sample from patients, 5-6 reference samples of red blood are needed. Our aim was to investigate how the mean corpuscular volume (MCV) of red blood cells influences on the value of fluorescence of bounded EMA dye and how the choice of reference samples affects the test result. EMA test was performed in peripheral blood from 404 individuals, including 31 children suffering from HS. Mean fluorescence channel of EMA-RBCs was measured with Cytomics FC500 flow cytometer. Mean corpuscular volume of RBCs was assessed with LH750 Beckman Coulter. Statistical analysis was performed using Graph Pad Prism. The correlation Spearman coefficient between mean channel of fluorescence of EMA-RBCs and MCV was r = 0.39, p EMA test depends on MCV of the reference samples. If reference blood samples have lower MCV than the patients MCV, EMA test result might be negative. Due to different MCV values of RBCs in infancy and ca. Three months later, EMA test in neonates might be interpreted falsely negative. Samples from children younger than 3 months old had EMA test result 86.1 ± 11.7 %, whereas same samples that analyzed 4.1 ± 2.1 later had results of 75.4 ± 4.5 %, p EMA-bound RBC depends on RBC's volume. MCV of reference samples affects EMA test results; thus, we recommend selection of reference samples with MCV in range of ±2 fL compared to MCV of patient RBC's.

  10. Nationwide Multicenter Reference Interval Study for 28 Common Biochemical Analytes in China.

    Science.gov (United States)

    Xia, Liangyu; Chen, Ming; Liu, Min; Tao, Zhihua; Li, Shijun; Wang, Liang; Cheng, Xinqi; Qin, Xuzhen; Han, Jianhua; Li, Pengchang; Hou, Li'an; Yu, Songlin; Ichihara, Kiyoshi; Qiu, Ling

    2016-03-01

    A nationwide multicenter study was conducted in the China to explore sources of variation of reference values and establish reference intervals for 28 common biochemical analytes, as a part of the International Federation of Clinical Chemistry and Laboratory Medicine, Committee on Reference Intervals and Decision Limits (IFCC/C-RIDL) global study on reference values. A total of 3148 apparently healthy volunteers were recruited in 6 cities covering a wide area in China. Blood samples were tested in 2 central laboratories using Beckman Coulter AU5800 chemistry analyzers. Certified reference materials and value-assigned serum panel were used for standardization of test results. Multiple regression analysis was performed to explore sources of variation. Need for partition of reference intervals was evaluated based on 3-level nested ANOVA. After secondary exclusion using the latent abnormal values exclusion method, reference intervals were derived by a parametric method using the modified Box-Cox formula. Test results of 20 analytes were made traceable to reference measurement procedures. By the ANOVA, significant sex-related and age-related differences were observed in 12 and 12 analytes, respectively. A small regional difference was observed in the results for albumin, glucose, and sodium. Multiple regression analysis revealed BMI-related changes in results of 9 analytes for man and 6 for woman. Reference intervals of 28 analytes were computed with 17 analytes partitioned by sex and/or age. In conclusion, reference intervals of 28 common chemistry analytes applicable to Chinese Han population were established by use of the latest methodology. Reference intervals of 20 analytes traceable to reference measurement procedures can be used as common reference intervals, whereas others can be used as the assay system-specific reference intervals in China.

  11. Time course characterization of serum cardiac troponins, heart fatty acid-binding protein, and morphologic findings with isoproterenol-induced myocardial injury in the rat.

    Science.gov (United States)

    Clements, Peter; Brady, Sally; York, Malcolm; Berridge, Brian; Mikaelian, Igor; Nicklaus, Rosemary; Gandhi, Mitul; Roman, Ian; Stamp, Clare; Davies, Dai; McGill, Paul; Williams, Thomas; Pettit, Syril; Walker, Dana; Turton, John

    2010-08-01

    We investigated the kinetics of circulating biomarker elevation, specifically correlated with morphology in acute myocardial injury. Male Hanover Wistar rats underwent biomarker and morphologic cardiac evaluation at 0.5 to seventy-two hours after a single subcutaneous isoproterenol administration (100 or 4000 microg/kg). Dose-dependent elevations of serum cardiac troponins I and T (cTnI, cTnT), and heart fatty acid-binding protein (H-FABP) occurred from 0.5 hour, peaked at two to three hours, and declined to baseline by twelve hours (H-FABP) or forty-eight to seventy-two hours (Serum cTns). They were more sensitive in detecting cardiomyocyte damage than other serum biomarkers. The Access 2 platform, an automated chemiluminescence analyzer (Beckman Coulter), showed the greatest cTnI fold-changes and low range sensitivity. Myocardial injury was detected morphologically from 0.5 hour, correlating well with loss of cTnI immunoreactivity and serum biomarker elevation at early time points. Ultrastructurally, there was no evidence of cardiomyocyte death at 0.5 hour. After three hours, a clear temporal disconnect occurred: lesion scores increased with declining cTnI, cTnT, and H-FABP values. Serum cTns are sensitive and specific markers for detecting acute/active cardiomyocyte injury in this rat model. Heart fatty acid-binding protein is a good early marker but is less sensitive and nonspecific. Release of these biomarkers begins early in myocardial injury, prior to necrosis. Assessment of cTn merits increased consideration for routine screening of acute/ongoing cardiomyocyte injury in rat toxicity studies.

  12. Comparable flow cytometry data can be obtained with two types of instruments, Canto II, and Navios. A GEIL study.

    Science.gov (United States)

    Solly, Françoise; Rigollet, Lauren; Baseggio, Lucille; Guy, Julien; Borgeot, Jessica; Guérin, Estelle; Debliquis, Agathe; Drenou, Bernard; Campos, Lydia; Lacombe, Francis; Béné, Marie C

    2013-12-01

    Flow cytometry (FC) instruments settings classically rely on local establishment of photomultipliers (PMT) voltages adapted to the measurements expected to be performed. In the era of multiparameter FC (MFC), it appears more and more desirable that comparable patterns of fluorescence are obtained in different settings. This relies on a harmonization of settings between instruments. Although this has been shown to be feasible within a given brand of flow cytometers, little information is available about broader comparisons in a given center or in a multicenter fashion. Here, we report a two-phase series of experiments first performed between a Canto II (BD Biosciences) and a Navios (Beckman Coulter) instruments in the same center. PMT values adjusted on the reference instrument (RI) Canto II were used to establish target values for PMT settings on the paired Navios practice instrument (PI). This allowed to show the good correlation of all but peaks 1 and 2 of Rainbow(®) beads between RI and PI. Using 4- or 8-color stained leukocytes, the similitude of the settings was further confirmed. A complex set of matrices was then established between five centers all equipped with both instruments. Using Bland & Altman difference comparisons for median fluorescence values, it was shown that using either Rainbow beads or CD16 stained polymorphonuclears to set-up target values on the RI CantoII, highly superimposable results could be obtained on all 9 PI. The latter were obtained using Rainbow beads or Compbeads(®) for comparisons. In summary, this two-phase study demonstrates the feasibility of different methods allowing for a robust harmonization of settings for MFC.

  13. Iron status and its determinants in a nationally representative sample of pregnant women.

    Science.gov (United States)

    Vandevijvere, Stefanie; Amsalkhir, Sihame; Van Oyen, Herman; Egli, Ines; Ines, Egli; Moreno-Reyes, Rodrigo

    2013-05-01

    Iron-deficiency anemia is associated with adverse neonatal health outcomes. Iron status and its determinants were assessed in a representative sample of Belgian pregnant women. Blood samples were collected and a questionnaire was completed face-to-face. Hemoglobin (Hb) and mean cell volume were measured using a Beckman Coulter Hematology Analyzer and serum ferritin (SF) and transferrin receptor (sTfr) concentrations by immunoassay. In total, 55 obstetric clinics and 1,311 pregnant women were included. Approximately 40% of third-trimester and 6% of first-trimester women had SF levels less than 15 μg/L. Approximately 21% of third-trimester and 4% of first-trimester women had anemia (Hb iron-deficient nonanemic (SF iron-deficiency anemia (SF iron deficiency (sTfr >8.5 mg/L). The median body iron stores were 8.1 mg/kg among first-trimester women, but only 3.6 mg/kg among third-trimester women. SF levels were significantly positively associated with age and education level, and were higher among nulliparous women and lower among North-African women. sTfr concentrations were significantly negatively associated with age and were lower among smokers, nulliparous women, and women who planned their pregnancy. Despite the fact that two thirds of Belgian pregnant women took iron-containing supplements, iron deficiency and iron-deficiency anemia were frequent in third-trimester women. The World Health Organization regards this as a moderate public health problem. National iron supplementation guidelines are needed in Belgium to optimize iron status during pregnancy.

  14. Diurnal Variations of Circulating Extracellular Vesicles Measured by Nano Flow Cytometry.

    Directory of Open Access Journals (Sweden)

    Kirsty M Danielson

    Full Text Available The identification of extracellular vesicles (EVs as intercellular conveyors of biological information has recently emerged as a novel paradigm in signaling, leading to the exploitation of EVs and their contents as biomarkers of various diseases. However, whether there are diurnal variations in the size, number, and tissue of origin of blood EVs is currently not known, and could have significant implications when using EVs as biomarkers for disease progression. Currently available technologies for the measurement of EV size and number are either time consuming, require specialized equipment, or lack sufficient accuracy across a range of EV sizes. Flow cytometry represents an attractive alternative to these methods; however, traditional flow cytometers are only capable of measuring particles down to 500 nm, which is significantly larger than the average and median sizes of plasma EVs. Utilizing a Beckman Coulter MoFlo XDP flow cytometer with NanoView module, we employed nanoscale flow cytometry (termed nanoFCM to examine the relative number and scatter distribution of plasma EVs at three different time points during the day in 6 healthy adults. Analysis of liposomes and plasma EVs proved that nanoFCM is capable of detecting biologically-relevant vesicles down to 100 nm in size. With this high resolution configuration, we observed variations in the relative size (FSC/SSC distributions and concentration (proportions of EVs in healthy adult plasma across the course of a day, suggesting that there are diurnal variations in the number and size distribution of circulating EV populations. The use of nanoFCM provides a valuable tool for the study of EVs in both health and disease; however, additional refinement of nanoscale flow cytometric methods is needed for use of these instruments for quantitative particle counting and sizing. Furthermore, larger scale studies are necessary to more clearly define the diurnal variations in circulating EVs, and thus

  15. Typing of 48 autosomal SNPs and amelogenin with GenPlex SNP genotyping system in forensic genetics.

    Science.gov (United States)

    Tomas, Carmen; Stangegaard, Michael; Børsting, Claus; Hansen, Anders Johannes; Morling, Niels

    2008-12-01

    GenPlex (Applied Biosystems) is a new SNP genotyping system based on an initial PCR amplification followed by an oligo ligation assay (OLA). The OLA consists of the hybridization of allele and locus specific oligonucleotides (ASOs and LSOs) to PCR products and posterior ligation of ASOs and LSOs. The ligation products are immobilized to microtitre plates and reporter oligonucleotides (ZipChute probes) are hybridized to the ligation products. ZipChute probes are subsequently eluted and detected using capillary electrophoresis. Applied Biosystems developed the GenPlex SNP genotyping system with amelogenin and 48 of the 52 SNPs used in the 52 SNP-plex assay developed by the SNPforID consortium. The system requires equipment that is usually found in forensic genetic laboratories. The use of a robot for performance of the pipetting steps is highly recommendable. A total of 286 individuals from Denmark, Somalia and Greenland were investigated with GenPlex using a Biomek 3000 (Beckman Coulter) robot. The results were compared to results obtained with an ISO 17025 accredited SNP typing assay based on single base extension (SBE). With the GenPlex SNP genotyping system, full SNP profiles were obtained in 97.6% of the investigations. Perfect concordance was obtained in duplicate investigations and the SNP genotypes obtained with the GenPlex system were concordant with those of the accredited SBE based SNP typing system except for one result in rs901398 in one of 286 individuals most likely due to a mutation 6 bp downstream of the SNP. Reproducible SNP genotypes were obtained from as little as 250 pg of DNA.

  16. Influence of HCl pretreatment on laser diffraction measurements

    Science.gov (United States)

    Schulte, Philipp; Steininger, Florian; Lockot, Gregori; Lehmkuhl, Frank; Stauch, Georg; Protze, Jens; Fischer, Peter

    2014-05-01

    Sample pretreatment methods in grain size (GS) analyses differ and their influence on GS distributions has been subject of controversial discussions. The standard sample preparation usually comprises the disaggregation into single primary particles. The organic binding material is oxidized by hydrogen peroxide (H2O2) and the containing carbonates are dissolved by hydrochloric acid (HCl). However, laser diffraction measurements of calcified sediment sequences or sediments with high contents of organic matter show non-reproducible changes in the GS distribution. To investigate variations of the GS distribution, selected samples from two different sections in different stages of weathering and sedimentary genesis were measured using a Beckman Coulter LS13320 laser particle size analyser. A high-resolution Holocene sandy loess-paleosol sequence, the Suohuduo section on the eastern margin of the Tibetan Plateau, was investigated. The results were compared with a Pleistocene loess sequence from the Lower Rhine Embayment, the Düsseldorf-Grafenberg section. The entire sample set includes samples of siliciclastic, barely weathered material and sediments from paleosols. The paleosols in the Suohudo section are strongly influenced by steppe fires and are rich in organo-mineral associations and pyrogenic carbon. All samples were pretreated with hydrogen peroxide and sodium pyrophosphate. In order to investigate the influence of HCl on the GS distribution, the samples were subsequently prepared with and without the addition of HCl. The results show that the sample preparation has a significant influence on the detected GS distribution. Hence, prior to the measurement of a sample set, the effectiveness of the pretreatment argents HCl and H2O2 should be evaluated. In order to generate a valid GS distribution, the sample pretreatment must be matched to the aim of the study and the composition of the sample. Paleoclimatic and environmental interpretation based on improper GS

  17. Comparative Analysis of Salivary Alkaline Phosphatase in Post menopausal Women with and without Periodontitis

    Science.gov (United States)

    Sophia, Khumukcham; Sudhakar, Uma; Jayakumar, Parvathee; Mathew, Danny

    2017-01-01

    Introduction Alkaline phosphatase is an intracellular destruction enzyme in the periodontium, and it takes part in the normal turnover of the periodontal ligament, alveolar bone, and root cementum formation and maintenance. Aim The aim of this case control study was to evaluate the enzyme Alkaline Phosphatase (ALP) level in saliva of post menopausal women with and without chronic periodontitis. Materials and Methods In this study, 40 individuals, satisfying the study inclusion and exclusion criteria, were recruited. They were categorically divided, on the basis of gingival index, probing pocket depth and clinical attachment level, into two groups: Group I (post menopausal women with a clinically healthy periodontium, n=20); and Group II (post menopausal women with generalized chronic periodontitis, n=20). Clinical parameters assessed were Plaque Index (PI), Gingival Index (GI), Clinical Attachment Level (CAL) and Probing Pocket Depth (PPD). Unstimulated salivary samples were obtained in which the ALP concentration was measured using p-Nitrophenylphosphate, and 2-amino-2-methyl-1-propanol reagents in Beckman and Coulter, AU 480 auto analyser. Mann-Whitney U test was used to find statistical difference with respect to all clinical parameters such as PI, GI, CAL, PPD and salivary ALP levels. Results The mean ALP in saliva was found to be higher in Group II compared to Group I and the difference was statistically significant with the p-value of 0.008. Conclusion A noteworthy increase in the ALP concentration was seen in saliva in our study (Group II) may be due to increased periodontal inflammation in post menopausal women. Thus salivary ALP can be taken as an additional biomarker to early diagnosis, development and progression of periodontitis especially among post menopausal women. PMID:28274061

  18. 不同流式细胞分析仪检测淋巴细胞亚群的比较研究%Comparison of three different flow cytometers in the clinical assessment of lymphocyte subsets

    Institute of Scientific and Technical Information of China (English)

    王维维; 奚迪; 袁向亮; 沈立松

    2016-01-01

    Objective To evaluate the consistency and accuracy among 3 brands of flow cytometers (BriCyte E6,BD FACSCanto Ⅱ and Beckman Coulter FC 500) in the detection of lymphocyte subsets.Methods According to the methodology,the BriCyte E6 was compared with 2 flow cytometers commonly used in clinical detection.Seventy-three cases (40 male and 33 female) of anticoagulation peripheral blood specimens were collected in the clinical laborartory department of Xinhua Hospital in July 2015 and the percentage (%) and absolute number (#) of the lymphocyte subsets were detected by 3 different flow cytometers within samples collected 4 h.Results There were good consistency among the 3 flow cytometers (R2 >0.95,R2 from 0.969 5 to 0.992 4) in the detection of lymphocyte subsets percentage,so did in the detection of absolute number (R2 > 0.95,R2 from 0.969 1 to 0.993 3).As to the precision evaluation,in the detectionof CD8%,T#,CD4+ T# and CD8+ T#,BriCyte E6 achieved a low CV% compared with FACSCanto Ⅱ and FC 500 (Friedman statistics are 16.720,11.840,15.760 and 15.430,P =0.000 2,0.027,0.000 4,0.000 4,respectively).In the detection of T%,CD4%,NK%,B%,NK#,B#,there was no significant difference among the 3 flow cytometers (Friedman statistics are 4.242,3.916,0.852,2.595,1.835 and 0.578,P =0.119 9,0.141 2,0.653 2,0.273 3,0.399 6,0.749 0,respectively).Conclusions The 3 flow cytometers have a good consistency in the detection of lymphocyte subsets.BriCyte E6 may be an alternative or complement of existing flow cytometers.%目的 通过对淋巴细胞亚群百分比及绝对计数的检测,对比3种流式细胞仪(BriCyteE6、BD FACSCanto Ⅱ、Beckman Coulter FC 500)在淋巴细胞亚群检测中的一致性和精密度.方法 按照方法学对比的要求,将BriCyte E6与2种临床常用的流式细胞仪进行比对.2015年7月连续5d随机收集上海交通大学医学院附属新华医院检验科抽取的新鲜肝素抗凝外周全血标本73

  19. A Study to Assess the Validity of Estimation of Serum Ostase Level in Hyperthyroid and Hypothyroid Cases

    Science.gov (United States)

    Boruah, Polina; Hajong, Ranendra; Nath, Chandan Kumar; Barman, Bhupen; Chutia, Happy; Sarma, Kalyan

    2016-01-01

    Introduction One of the more specific assessments of the metabolic status of bone in normal and in disease conditions is the measurement of bone specific alkaline phosphatase or ostase. The measurement of serum ostase has several advantages over the measurements of other bone parameter. Because of its relatively long half-life, in-vivo (1 to 3days), it is relatively unaffected by diurnal variation. Aim To find the correlation of serum ostase level in hyper and hypothyroid cases and also to study the validity of routine estimation of serum ostase in hyper and hypothyroid cases so as to monitor the base level bone health on presentation. Materials and Methods Serum ostase level was studied in 74 patients with disorder of thyroid function. Serum ostase level, Thyroid Stimulating Hormone (TSH), FT3, FT4 levels were estimated by chemiluminescent technique. The instrument used was Beckman- coulter Access 2. A total of 39 patients were hypothyroid, 31 were hyperthyroid and 4 patients had subclinical hyperthyroidism. Results The serum ostase level was found to be elevated above 40 μg/L in 26 of the cases and above 16 μg/L but below 40μg/L in 5 cases of hyperthyroidism along with decrease in Bone Mineral Density (BMD). Serum ostase level was found to be directly proportional to the serum FT3 level (Normal range of serum ostase is 8-16 μg/L). Conclusion From this study, an inference can be drawn that a routine estimation of serum ostase level in hyperthyroid cases will help in proper monitoring of decrease bone turnover as indicated by increase serum ostase level. Besides, the estimation of serum ostase level in hyperthyroid cases it is found to be valid in this study, which can turn to be an important guiding parameter to the treating physician to formulate necessary protocols and guidelines for prophylaxis, treatment and to monitor the response to therapy in cases of reduced bone turnover related to hyperthyroid state. PMID:27790421

  20. Study on the relationship between the serum antibody IgG of egg, milk and the common index in health examination population%体检人群牛奶、鸡蛋IgG抗体水平与常见体检指标的关系

    Institute of Scientific and Technical Information of China (English)

    冯琳琳; 张祁; 张新娟; 郑艳颖; 张微

    2012-01-01

    Objective: To investigate the relationship between IgG levels in egg, milk and common indexes like blood glucose, lipids in health examination population. Methods: Enzyme -linked immunosorbent assay (ELISA) was used to detect the serum IgG antibodies of egg and milk in 3864 cases of physical examination, and serum lipids and blood glucose were detected by Beckman Coulter C800. Results: The positive rates of egg and milk intolerance were 38% and 25% respectively. The ratio of abnormal blood glucose and lipids were correlated with the eggs and milk antibody levels. Conclusion: The rate of the egg or milk intolerance were high in our subjects, especially in female. The combined detection of food intolerance levels, blood sugar, blood lipid levels have important significance in the disease diagnosis and health guidance on diet.%目的:探索体检人群血清中鸡蛋、牛奶IgG水平与常规体检指标的关系.方法:采用酶联免疫吸附法检测3864例体检者血清中牛奶、鸡蛋的特异性IgG抗体,同时检测空腹血糖、TG、TCHO、LDL和HDL指标.结果:受检人群中鸡蛋不耐受阳性率38%,牛奶不耐受阳性率25%,女性阳性率均高于男性.受检人群血糖、TG异常比例随鸡蛋和牛奶抗体水平上升而下降,HDL异常比例随鸡蛋和牛奶抗体水平上升而上升,TCHO异常比例随鸡蛋抗体水平上升而下降.结论:国内人群鸡蛋,牛奶不耐受发生率高;血糖,血脂异常与鸡蛋、牛奶不耐受程度有关.

  1. A simple cost-effective high performance liquid chromatographic assay of sulphadoxine in whole blood spotted on filter paper for field studies

    Directory of Open Access Journals (Sweden)

    Sijuade Abayomi O

    2009-10-01

    Full Text Available Abstract Background Artesunate plus sulphadoxine-pyrimethamine is one of the four artemisinin-based combination therapies currently recommended by WHO as first-line treatment for falciparum malaria. Sulphadoxine-pyrimethamine is also used for intermittent preventive treatment for malaria in pregnancy. Drug use patterns and drug pharmacokinetics are important factors impacting the spread of drug resistant parasites hence it is imperative to monitor the effect of pharmacokinetic variability on therapeutic efficacy. Unfortunately, information on the pharmacokinetics of sulphadoxine in children and pregnant women with malaria is very limited. Methods for the assay of sulphadoxine-pyrimethamine have been previously reported, but they are not cost-effective and practicable in analytical laboratories in low resource areas where malaria is endemic. Efforts in this study were thus devoted to development and evaluation of a simple, cost-effective and sensitive method for quantification of sulphadoxine in small capillary samples of whole blood dried on filter paper. Methods Sulphadoxine was determined in whole blood by reversed-phase high performance liquid chromatography with UV detection at 340 nm. Sulisoxazole (SLX was used as internal standard. Chromatographic separation was achieved using a Beckman Coulter ODS C18 and a mobile phase consisting of 0.05 M phosphate buffer-methanol-acetonitrile (70:17:13 V/V/V containing 1% triethylamine solution. Results Standard curves from sulphadoxine-spiked blood added to filter paper were linear over the concentration range studied. Linear regression analysis yielded correlation coefficient r2 > 0.99 (n = 6. Extraction recoveries were about 82-85%. The limit of quantification was 120 ng/ml while the within and between assay coefficient of variations were Conclusion The recovery and accuracy of determination of SDX from whole blood filter paper samples using the method described in this study is satisfactory, thus

  2. Online analysis of europium and gadolinium species complexed or uncomplexed with humic acid by capillary electrophoresis-inductively coupled plasma mass spectrometry.

    Science.gov (United States)

    Kautenburger, Ralf; Nowotka, Karsten; Beck, Horst Philipp

    2006-03-01

    Detailed information on the geochemical behavior of radioactive and toxic metal ions under environmental conditions (in geological matrices and aquifer systems) is needed in order to assess the long-term safety of waste repositories. This includes knowledge of the mechanisms of relevant geochemical reactions, as well as associated thermodynamic and kinetic data. Several previous studies have shown that humic acid can play an important role in the immobilization or mobilization of metal ions due to complexation and colloid formation. In our project we investigate the complexation behavior of (purified Aldrich) humic acid and its influence on the migration of the lanthanides europium and gadolinium (homologs of the actinides americium and curium) in the ternary system consisting of these heavy metals, humic acid and kaolinite (KGa-1b) under almost natural conditions. Capillary electrophoresis (CE, Beckman Coulter P/ACE MDQ), with its excellent separation performance, was hyphenated with a homemade interface to inductively coupled plasma mass spectrometry (ICP-MS, VG Elemental PlasmaQuad 3) giving a system that is highly sensitive to the rare-earth element species of europium and gadolinium with humic acid. The humic acid used was also halogenated with iodine, which acted as an ICP-MS marker. To couple CE to ICP-MS, a fused silica CE capillary was flexibly fitted into a MicroMist 50 mul nebulizer with a Cinnabar cyclonic spray chamber in the external homemade interface. The chamber was chilled to a temperature of 4 degrees C to optimize the sensitivity. 200 ppb of cesium were added to the CE separation buffer so that the capillary flow could be observed. A make-up fluid including 4 ppb Ho as an internal standard was combined with the flow from the capillary within the interface in order to get a fluid throughput high enough to maintain continuous nebulization. Very low detection limits were achieved: 125 ppt for 153Eu and 250 ppt for 158Gd. Using this optimized CE

  3. Environmental change indicated by grain-size variations and trace elements: An example from the sandy-loess sediments from the Doroshivtsy site (Ukraine)

    Science.gov (United States)

    Lehmkuhl, Frank; Schulte, Philipp; Loibl, Christa; Klasen, Nicole; Hauck, Thomas; Kels, Holger

    2014-05-01

    The recently discovered loess section of Doroshivtsy became famous due to archeological findings of the Gravettian. The profile represents a about 9 m sequence of sandy loess, intercalated by more humic horizons. It is situated in a flat gully and at an undercut slope of the Dniester River close to the village Doroshivtsy in the south-western Ukraine. Radiocarbon dating and luminescence ages are conclusive within the expected age range of the Gravettian period (22-28 ka). The loess section is composed mainly of sandy silt and covers the time span from about 26 to 16 ka. This is one of the very few sections in Europe which provides a high resolution sedimentary record including prehistoric finds of the cooling maximum of the last glacial cycle. Based on the field description and the grain size distribution obtained by Laser Diffraction Particle Size Analyzer (Beckman Coulter LS 13 320 PIDS), the profile can be divided into three main genetic units representing changes during the deposition. The upper part (1-3 m) of the profile is characterized by aeolian loess and sand deposition intercalated with a few gravel lines. The middle part (3-6.5 m) of the profile is influenced by aeolian, denudative and weak soil forming processes. The lower part (6.5-9 m) of the section represents a combination of aeolian loess, re-deposited slope material. Additionally, the whole part is affected by hydromorphic conditions. As the sediment is rather homogeneous sandy silt U-ratio and GSI did not show any distinct variations. However, calculations with different grain-size ratios show that a fine silt to fine sand ratio (SiS-ratio) of 3.5 to 8.1 µm and 69.6 to 161.1 µm provide clear peaks. These variations of the SiS-ratio represent environmental changes which are also observed by structures and weak soil formations in the section during field work and fit also to the different archeological layers. In addition, geochemical analysis show comparable results to the SiS-ratio and provide

  4. Bone marrow mononuclears from murine tibia after spaceflight on biosatellite

    Science.gov (United States)

    Andreeva, Elena; Roe, Maria; Buravkova, Ludmila; Andrianova, Irina; Goncharova, Elena; Gornostaeva, Alexandra

    Elucidation of the space flight effects on the adult stem and progenitor cells is an important goal in space biology and medicine. A unique opportunity for this is provided by project "BION -M1". The purpose of this study was to evaluate the effects of a 30-day flight on biosatellite "BION - M1" and the subsequent 7-day recovery on the quantity, viability, immunophenotype of mononuclears from murine tibia bone marrow. Also the in vitro characterization of functional capacity of multipotent mesenchymal stromal cells (MSCs) was scheduled. Under the project, the S57black/6 mice were divided into groups: spaceflight/vivarium control, recovery after spaceflight/ vivarium control to recovery. Bone marrow mononuclears were isolated from the tibia and immunophenotyped using antibodies against CD45, CD34, CD90 on a flow cytometer Epics XL (Beckman Coulter). A part of the each pool was frozen for subsequent estimation of hematopoietic colony-forming units (CFU), the rest was used for the evaluation of fibroblast CFU (CFUf) number, MSC proliferative activity and osteogenic potency. The cell number in the flight group was significantly lower than in the vivarium control group. There were no differences in this parameter between flight and control groups after 7 days of recovery. The mononuclears viability was more than 95 percent in all examined groups. Flow cytometric analysis showed no differences in the bone marrow cell immunophenotype (CD45, CD34, CD90.1 (Thy1)), but the flight animals had more large-sized CD45+mononuclears, than the control groups of mice. There was no difference in the CFUf number between groups. After 7 days in vitro the MSC number in flight group was twice higher than in vivarium group, after 10 days - 4 times higher. These data may indicate a higher proliferative activity of MSCs after spaceflight. MSCs showed the same and high alkaline phosphatase activity, both in flight and in the control groups, suggesting no effect of spaceflight factors on early

  5. Severe malnutrition with and without HIV-1 infection in hospitalised children in Kampala, Uganda: differences in clinical features, haematological findings and CD4+ cell counts

    Directory of Open Access Journals (Sweden)

    Downing Robert

    2006-10-01

    Full Text Available Abstract Background The aim of this study was to describe the clinical features, haematological findings and CD4+ and CD8+ cell counts of severely malnourished children in relation to human immunodeficiency virus (HIV infection. Methods The study was conducted in the paediatric wards of Mulago hospital, which is Uganda's national referral and teaching hospital. We studied 315 severely malnourished children (presence of oedema and/or weight-for-height: z-score + and CD8+ cells were measured by the flow cytometry and HIV serology was confirmed by Enzyme linked Immunoassay for children >18 months of age, and RNA PCR was performed for those ≤18 months. Complete blood count, including differential counts, was determined using a Beckman Coulter counter. Results Among the 315 children, 119 (38% were female; the median age of these children was 17 months (Interquartile range 12–24 months, and no difference was observed in the HIV status with regard to gender or age. The children showed a high prevalence of infections: pneumonia (68%, diarrhoea (38%, urinary tract infection (26% and bacteraemia (18%, with no significant difference with regard to the HIV status (HIV-positive versus HIV-negative children. However, the HIV-positive children were more likely to have persistent diarrhoea than the HIV-uninfected severely malnourished children (odds ratio (OR 2.0, 95% confidence interval (CI 1.2–3.6. When compared with the HIV-negative children, the HIV-positive children showed a significantly lower median white blood cell count (10700 versus 8700 and lymphocyte count (4033 versus 2687. The CD4+ cell percentages were more likely to be lower in children with non-oedematous malnutrition than in those with oedematous malnutrition even after controlling for the HIV infection. The novel observation of this study is that the CD4+ percentages in both HIV-positive and HIV-negative children without oedema were lower that those in children with oedema. These

  6. Evaluation of Oxacillin and Cefoxitin Disk and MIC Breakpoints for Prediction of Methicillin Resistance in Human and Veterinary Isolates of Staphylococcus intermedius Group

    Science.gov (United States)

    Wu, M. T.; Westblade, L. F.; Dien Bard, J.; Wallace, M. A.; Stanley, T.; Burd, E.; Hindler, J.

    2015-01-01

    Staphylococcus pseudintermedius is a coagulase-positive species that colonizes the nares and anal mucosa of healthy dogs and cats. Human infections with S. pseudintermedius range in severity from bite wounds and rhinosinusitis to endocarditis; historically, these infections were thought to be uncommon, but new laboratory methods suggest that their true incidence is underreported. Oxacillin and cefoxitin disk and MIC tests were evaluated for the detection of mecA- or mecC-mediated methicillin resistance in 115 human and animal isolates of the Staphylococcus intermedius group (SIG), including 111 Staphylococcus pseudintermediusand 4 Staphylococcus delphini isolates, 37 of which were mecA positive. The disk and MIC breakpoints evaluated included the Clinical and Laboratory Standards Institute (CLSI) M100-S25 Staphylococcus aureus/Staphylococcus lugdunensis oxacillin MIC breakpoints and cefoxitin disk and MIC breakpoints, the CLSI M100-S25 coagulase-negative Staphylococcus (CoNS) oxacillin MIC breakpoint and cefoxitin disk breakpoint, the CLSI VET01-S2 S. pseudintermedius oxacillin MIC and disk breakpoints, and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) S. pseudintermedius cefoxitin disk breakpoint. The oxacillin results interpreted by the VET01-S2 (disk and MIC) and M100-S25 CoNS (MIC) breakpoints agreed with the results of mecA/mecC PCR for all isolates, with the exception of one false-resistant result (1.3% of mecA/mecC PCR-negative isolates). In contrast, cefoxitin tests performed poorly, ranging from 3 to 89% false susceptibility (very major errors) and 0 to 48% false resistance (major errors). BD Phoenix, bioMérieux Vitek 2, and Beckman Coulter MicroScan commercial automated susceptibility test panel oxacillin MIC results were also evaluated and demonstrated >95% categorical agreement with mecA/mecC PCR results if interpreted by using the M100-S25 CoNS breakpoint. The Alere penicillin-binding protein 2a test accurately detected all

  7. Evaluation of analytical performance and comparison of clinical results of the new generation method AccuTnI+3 for the measurement of cardiac troponin I using both patients and quality control plasma samples.

    Science.gov (United States)

    Storti, Simona; Masotti, Silvia; Prontera, Concetta; Franzini, Maria; Buzzi, Paola; Casagranda, Ivo; Ciofini, Enrica; Zucchelli, Gian Carlo; Ndreu, Rudina; Passino, Claudio; Clerico, Aldo

    2015-12-07

    The study aims are to evaluate the analytical performance and the clinical results of the chemiluminescent Access AccuTnI+3 immunoassay for the determination of cardiac troponin I (cTnI) with DxI 800 and Access2 platforms and to compare the clinical results obtained with this method with those of three cTnI immunoassays, recently introduced in the European market. The limits of blank (LoB), detection (LoD), and quantitation (LoQ) at 20% CV and 10% CV were 4.5 ng/L and 10.9 ng/L, 17.1 and 30.4 ng/L, respectively. The results of STAT Architect high Sensitive TnI (Abbott Diagnostics), ADVIA Centaur Troponin I Ultra (Siemens Healthcare Diagnostics), ST AIA-Pack cTnI third generation (Tosoh Bioscience), and Access AccuTnI+3 (Beckman Coulter Diagnostics) showed very close correlations (R ranging from 0.901 to 0.994) in 122 samples of patients admitted to the emergency department. However, on average there was a difference up to 2.4-fold between the method measuring the highest (ADVIA method) and lowest cTnI values (AccuTnI+3 method). The consensus mean values between methods ranged from 6.2% to 29.6% in 18 quality control samples distributed in an external quality control study (cTnI concentrations ranging from 29.3 ng/L to 1557.5 ng/L). In conclusion, the results of our analytical evaluation concerning the AccuTnI+3 method, using the DxI platform, are well in agreement with those suggested by the manufacturer as well as those reported by some recent studies using the Access2 platform. Our results confirm that the AccuTnI+3 method for the Access2 and DxI 800 platforms is a clinically usable method for cTnI measurement.

  8. Comparison and evaluation of seven different bench-top flow cytometers with a modified six-plexed mycotoxin kit.

    Science.gov (United States)

    Czeh, Arpad; Schwartz, Abe; Mandy, Frank; Szoke, Zsuzsanna; Koszegi, Balazs; Feher-Toth, Szilvia; Nagyeri, Gyorgy; Jakso, Pal; Katona, Robert L; Kemeny, Agnes; Woth, Gabor; Lustyik, Gyorgy

    2013-12-01

    Many bench-top flow cytometers (b-FCs) are compatible with microsphere-based multiplexed assays. Disciplines implementing b-FCs-based assays are expanding; they include monitoring and validating food quality. A multiplexed platform protocol was evaluated for poly-mycotoxin assays, which is compatible with a variety of b-FC models. The seven instruments included: BD FACSCalibur(™) , BD FACSArray(™) Bioanalyzer, Accuri C6, Partec CyFlow(®) Space, Beckman Coulter FC 500, Guava EasyCyte Mini, and Luminex 100 (™) . Current reports related to the food industry describe fungal co-infections leading to poly-mycotoxin contamination in grain (Sulyok M, Berthiller F, Krska R, Schuhmacher R, Rapid Commun Mass Spectrom 2006;20:2649-2659). It is imperative to determine whether b-FC-based assays can replace traditional single-mycotoxin enzyme-linked immunosorbent assay (ELISA). A six-plexed poly-mycotoxin kit was tested on seven different b-FCs. The modified kit was initially developed for the BD FACSArray(™) Bioanalyzer (BD Biosciences) (Czeh A, Mandy F, Feher-Toth S, Torok L, Mike Z, Koszegi B, Lustyik G, J Immunol Methods 2012;384:71-80). With the multiplexed platform, it is possible to identify up to six mycotoxin contaminants simultaneously at regional grain collection/transfer/inspection facilities. In the future, elimination of contaminated food threat may be better achieved with the inclusion of b-FCs in the food protection arsenal. A universal protocol, matched with postacquisition software, offers an effective alternative platform compared to using a series of ELISA kits. To support side-by-side evaluation of seven flow cytometers, an instrument-independent fluorescence emission calibration was added to the protocol. All instrument performances were evaluated for strength of agreement based on paired sets of evaluation to predicate method. The results suggest that all b-FCs were acceptable of performing with the multiplexed kit for five of six mycotoxins. For

  9. Evaluation of Oxacillin and Cefoxitin Disk and MIC Breakpoints for Prediction of Methicillin Resistance in Human and Veterinary Isolates of Staphylococcus intermedius Group.

    Science.gov (United States)

    Wu, M T; Burnham, C-A D; Westblade, L F; Dien Bard, J; Lawhon, S D; Wallace, M A; Stanley, T; Burd, E; Hindler, J; Humphries, R M

    2016-03-01

    Staphylococcus pseudintermedius is a coagulase-positive species that colonizes the nares and anal mucosa of healthy dogs and cats. Human infections with S. pseudintermedius range in severity from bite wounds and rhinosinusitis to endocarditis; historically, these infections were thought to be uncommon, but new laboratory methods suggest that their true incidence is underreported. Oxacillin and cefoxitin disk and MIC tests were evaluated for the detection of mecA- or mecC-mediated methicillin resistance in 115 human and animal isolates of the Staphylococcus intermedius group (SIG), including 111 Staphylococcus pseudintermediusand 4 Staphylococcus delphini isolates, 37 of which were mecA positive. The disk and MIC breakpoints evaluated included the Clinical and Laboratory Standards Institute (CLSI) M100-S25 Staphylococcus aureus/Staphylococcus lugdunensis oxacillin MIC breakpoints and cefoxitin disk and MIC breakpoints, the CLSI M100-S25 coagulase-negative Staphylococcus (CoNS) oxacillin MIC breakpoint and cefoxitin disk breakpoint, the CLSI VET01-S2 S. pseudintermedius oxacillin MIC and disk breakpoints, and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) S. pseudintermedius cefoxitin disk breakpoint. The oxacillin results interpreted by the VET01-S2 (disk and MIC) and M100-S25 CoNS (MIC) breakpoints agreed with the results of mecA/mecC PCR for all isolates, with the exception of one false-resistant result (1.3% of mecA/mecC PCR-negative isolates). In contrast, cefoxitin tests performed poorly, ranging from 3 to 89% false susceptibility (very major errors) and 0 to 48% false resistance (major errors). BD Phoenix, bioMérieux Vitek 2, and Beckman Coulter MicroScan commercial automated susceptibility test panel oxacillin MIC results were also evaluated and demonstrated >95% categorical agreement with mecA/mecC PCR results if interpreted by using the M100-S25 CoNS breakpoint. The Alere penicillin-binding protein 2a test accurately detected all

  10. Measurement of particle size distribution of soil and selected aggregate sizes using the hydrometer method and laser diffractometry

    Science.gov (United States)

    Guzmán, G.; Gómez, J. A.; Giráldez, J. V.

    2010-05-01

    Soil particle size distribution has been traditionally determined by the hydrometer or the sieve-pipette methods, both of them time consuming and requiring a relatively large soil sample. This might be a limitation in situations, such as for instance analysis of suspended sediment, when the sample is small. A possible alternative to these methods are the optical techniques such as laser diffractometry. However the literature indicates that the use of this technique as an alternative to traditional methods is still limited, because the difficulty in replicating the results obtained with the standard methods. In this study we present the percentages of soil grain size determined using laser diffractometry within ranges set between 0.04 - 2000 μm. A Beckman-Coulter ® LS-230 with a 750 nm laser beam and software version 3.2 in five soils, representative of southern Spain: Alameda, Benacazón, Conchuela, Lanjarón and Pedrera. In three of the studied soils (Alameda, Benacazón and Conchuela) the particle size distribution of each aggregate size class was also determined. Aggregate size classes were obtained by dry sieve analysis using a Retsch AS 200 basic ®. Two hundred grams of air dried soil were sieved during 150 s, at amplitude 2 mm, getting nine different sizes between 2000 μm and 10 μm. Analyses were performed by triplicate. The soil sample preparation was also adapted to our conditions. A small amount each soil sample (less than 1 g) was transferred to the fluid module full of running water and disaggregated by ultrasonication at energy level 4 and 80 ml of sodium hexametaphosphate solution during 580 seconds. Two replicates of each sample were performed. Each measurement was made for a 90 second reading at a pump speed of 62. After the laser diffractometry analysis, each soil and its aggregate classes were processed calibrating its own optical model fitting the optical parameters that mainly depends on the color and the shape of the analyzed particle. As a

  11. 妊娠中期母血清唐氏综合征三联筛查4 680例与不良妊娠结果分析%Chromosomal abnormalities and adverse pregnancy outcome with maternal serum second trimester triple screening test for fetal Down syndrome in 4 860 Chinese women

    Institute of Scientific and Technical Information of China (English)

    夏燕萍; 朱铭伟; 李笑天; 周和平; 王静; 吕菊香; Nanbert ZHONG

    2006-01-01

    Objective:To investigate the efficiency of maternal serum triple screening for the genetic abnormality in second-trimester and the morbidity of adverse pregnancy outcome in false positive results of the test. Methods: A total of 4 680 pregnant women with singleton pregnancies assigned in Obs & Gyn Hospital, Fudan University, underwent triple screening test (alpha fetoprotein, AFP; human chorionic gonadotropin, HCG and unconjugated estriol, uE3) by fluorescence enzyme immunoassay between 2003 and 2005. The valid MoM (Multiples of Median) value of mid-trimester serum AFP, uE3, and hCG and risk assessments was provided by Beckman Coulter Co. When applied in the prenatal Down syndrome screening service. The study compares the incidence of chromosomal abnormalities with Down syndrome in screen positive women and compares to the MoM value established in the literature. The risks of having a fetus with congenital abnormalities or of developing obstetric complications in the screen positive women with their matched controls.Results:The MoM values for the triple tests of our study are similar to established values of literature. Only 51.01% women with pregnancies agree to receive screening. Amniocentesis utilization rate was 55.12% in the screen-positive pregnancies. The false positive rate was 6.89% and the median of maternal age of the women was 28.13 (range 19 to 49) years old. Chromosomal abnormalities were identified in 21 pregnancies, including 9 cases of trisomy 21.The detection rate was 77.77%. Pregnancies with positive screening results had a significantly higher risk of adverse outcomes than those with negative results (P< 0.05). Whereas there was no difference in the incidences of fetal congenital appearance or skeleton abnormality. Conclusion: Adjusting MoM values of local unaffected populations is limited to increasing the detection rate. Because chromosomal defects have variable exhibitions, amniocentesis utilization is still a choice for screen

  12. Clinical evaluation of leukocyte differential count in peripheral blood by five-color flow cytometry%五色流式细胞术分类计数外周血白细胞的临床评价

    Institute of Scientific and Technical Information of China (English)

    邢莹; 王建中; 普程伟; 尚柯

    2013-01-01

    Peking University First Hospital from September 2010 to December 2010 was detected by standard microscopic cytology as a reference method.Meanwhile,Beckman-Coulter LH750 hematology analyzer and FC500 flow cytometer were performed.Then the correlations were analyzed between microscopic cytology,hematology analyzer and flow cytometry.Forty blood samples collected at Peking University First Hospital,Beijing Daopei Hospital and General Hospital of Beijing Military Command from August 2010 to November 2010 were analyzed by standard microscopic cytology,Beckman-Coulter LH750 hematology analyzer and NAVIOS flow cytometer.Then the correlations between microscopy,hematology analyzer and flow cytometry were explored to analyze the clinical diagnostic efficiency of flow cytometry.Results Correlation of leukocyte differential count between FC500 flow cytometer and standard microscopic cytology was significant(all P < 0.01).And it was superior in the detection of lymphocytes,neutrophils and eosinaphils (r =0.955,0.928,0.876).Moreover,the correlation of leukocyte differential count between NAVIOS flow cytometer via manual gate and standard microscopic cytology was significant (r > 0.700,all P < 0.01) except for basophils.And it was superior in the detection of neutrophils,lymphocytes and blasts (r =0.950,0.915,0.852).When 1% was set as the cut-off value of immature granulocytes on standard microscopic cytology,the sensitivity and specificity of flow cytometry was 87% and 76% respectively.When 0.5% was set as the cut-off value of blasts on standard microscopic cytology,the sensitivity and specificity of flow cytometry stood at 100% and 92% respectively.Conclusion Five-color flow cytometry is wellcorrelated with standard microscopic cytology for leukocyte differential count in peripheral blood with different flow cytometers,and the sensitivity of detecting blasts and immature granulocytes is very excellent.

  13. Human umbilical cord-derived mesenchymal stem cell tansplantation for liver fibrosis and cirrhosis%脐带源间充质干细胞移植治疗肝纤维化及肝硬化的相关机制

    Institute of Scientific and Technical Information of China (English)

    孙慧聪; 张国尊; 郭金波; 冯燕; 郑力搏; 张晓岚

    2015-01-01

    背景:肝硬化是多种原因引起的肝脏慢性病变,目前尚没有有效的治疗方法,很多研究表明,间充质干细胞对肝纤维化及肝硬化有一定的治疗作用。目的:研究人脐带源间充质干细胞移植对大鼠肝纤维化及肝硬化的治疗作用及其作用机制。方法:应用CCl4诱导制备肝纤维化及肝硬化模型,造模后经尾静脉注射人脐带间充质干细胞。细胞移植后采用Beckman Coulter analyzer检测人脐带源间充质干细胞移植对大鼠肝功能的影响;采用天狼猩红染色检测肝组织病理改变;应用免疫组织化学染色、Western blot和real-time Q-PCR方法检测Ⅰ、Ⅲ型胶原、基质金属蛋白酶2、基质金属蛋白酶抑制剂2蛋白与mRNA在大鼠肝组织中的表达。结果与结论:人脐带源间充质干细胞移植可以改善肝纤维化及肝硬化大鼠的肝功能。人脐带源间充质干细胞移植后,除肝纤维化细胞移植1周组与对应模型组相比差异无显著性意义外,其余各细胞移植组肝脏组织中基质金属蛋白酶2 mRNA及蛋白表达水平明显升高,而Ⅰ、Ⅲ型胶原、基质金属蛋白酶抑制剂2表达水平明显降低。人脐带源间充质干细胞通过上调基质金属蛋白酶2表达,下调基质金属蛋白酶抑制剂2表达,对肝纤维化及肝硬化起到治疗作用;在致病因素持续存在的情况下,人脐带源间充质干细胞移植并不能逆转肝纤维化或者肝硬化,只能延缓肝纤维化或肝硬化的进程。%BACKGROUND:Cirrhosis is a long-term consequence of chronic hepatic injury, which has no effective therapy. Mesenchymal stem cels have been shown to play a potential role in the treatment of liver fibrosis/cirrhosis. OBJECTIVE:To investigate the therapeutic effect and mechanism of human umbilical cord-derived mesenchymal stem cels on CCl4 induced liver fibrosis/cirrhosis in rats. METHODS:A CCl4-induced liver fibrotic

  14. Evaluation of VCS technology in diagnosis for leukemia%VCS技术在白血病诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    徐双; 刘健; 赵磊; 贾玫; 刘宁

    2013-01-01

    Objective To evaluate clinical application of VCS technology in diagnosis for leukemia and its subtypes by Beckman Coulter LH750 automated hematology analyzer. Methods Peripheral blood samples from 112 healthy sub jeets and 163 Leukemia patients were collected,then all the samples were analyzed by Coulter LH750 automated hema tology analyzer to get the white blood cell VCS parameters . The cutoff values with high sensitivity and specificity were chosen by receiver operating characteristic (ROC) curves in leukemia and chronic myelogenous leukemia(CML). The VCS parameters of different acute myelogenous leukemia (AML) subtypes were compared. Results To discriminate the healthy subjects and the leukemia, the VCS parameters except MLV、MLS、 MEC、MES-SD showed a statistical sig nificance,and MNC-SD、MNV-SD、MLC-SD are the best indicators in distinguishing healthy subjects and Leukemia pa tients. When the cutoff value of MNC-SD get 7.23,a sensitivity of 88.7% and specificity of 92.3% were achieved;When the cutoff value of MNV SD get23.35,a sensitivity of 83.4% and specificity of 87.6% were achieved;When the cutoff value of MLC-SD get 11.54,a sensitivity of 88.9% and specificity of 97.7% were achieved. To distinguishing AML and CML, the parameters of WBC、 MLV-SD MMV-SD、MEV、 MEV-SD、 MNS、 MMS、 MES had a statistical significance . By ROC curves, three parameters with high sensitivity and specificity were chosen: When the cutoff value of WBC get 34.5,a sensitivity of 67.9% and specificity of 63.9% were achieved;When the cutoff value of MNS get120.8,a sensitiv ity of 60.4% and specificity of 61. l% were achieved; When the cutoff value of MEV get 164.5,a sensitivity of 71.7% and specificity of 70.3% were achieved. And combined WBC、MNS、MEV、any two of the three or all the three parame ters over the cutoff value, a sensitivity of 84.9% (45/53)and specificity of 80.8% (63/78)were achieved. The differences of MNV、MNC-SD、MLC、 MMS、 MMC-SD for different

  15. Acoustic Correlates of Devoiced Japanese Vowels : Velar Context

    OpenAIRE

    VARDEN, J. Kevin

    2010-01-01

    This paper reports preliminary findings of research on the acoustic characteristics of voiceless obstruent/devoiced vowel sequences in Japanese. The starting point for the investigation is Beckman & Shoji (1984), who detailed the characteristics of /∫/ be

  16. An EPR Study of the Thermal Decomposition of TNT/HMB Mixtures

    Science.gov (United States)

    1985-08-22

    8217 .’..’ .. ... , .-. .... ... .. ". .... .,. *-- .. . ..--.. ’. ,* * - .*.. ... .... ,. .. -.- ,. .. ’ V. REFEE1EC.S 1. a. J. W. Beckman, J. S. Wilkes, and R. R. McGuire, Thermochimica Acta , 19

  17. The establishment of pediatric reference intervals for creatine kinase-MB and troponin Ⅰ%肌钙蛋白Ⅰ和肌酸激酶同工酶儿童参考区间的建立

    Institute of Scientific and Technical Information of China (English)

    李怀远; 蒋黎敏; 郑建新; 孙璇; 傅启华

    2012-01-01

    Objective To establish the pediatric reference intervals for Troponin Ⅰ and Creatine kinase-MB (CK-MB).Methods Healthy children (223 boys and 162 girls) aged from 7 months to 12 years old were studied.Blood specimens were collected and centrifuged,all serum samples were kept refrigerated below-70 ℃.Troponin Ⅰ and CK-MB were measured on the UniCel DxI 800 immunoassay system (Beckman Coulter) on the same day.Nonparametric statistics was used to estimate the 99th percentile reference intervals.Results The children were divided into three groups (group A 128 cases; group B 128 cases;group C 129 cases) according to age.The upper reference limits for CK-MB were dependent of age,and the group A (7 months-1 years) was shown to be 13.41 μg/L,the group B (2-3 years) was shown to be 9.35 μg/L,the group C (4-12 years) was shown to be 5.48 μg/L.The upper reference limit for Troponin Ⅰ was independent of age or gender and shown to be 0.01 μg/L among 385 children.Conclusions Pediatric reference intervals for Troponin Ⅰ and CK-MB have been defined in healthy children at a relevant age group (7 months-12 years).It is effective for clinical diagnosis and treatment of myocardial damage for children by determining reference intervals for Troponin Ⅰ and CK-MB.%目的 建立肌钙蛋白Ⅰ(Troponin Ⅰ)和肌酸激酶同工酶(CK-MB)儿童参考区间.方法 2011年8-12月收集上海儿童医学中心年龄7个月至12岁健康体检儿童385名,其中男223名,女162名.血液收集后离心,血清-70℃冷冻保存,使用贝克曼库尔特UniCel DxI 800免疫分析系统同一天集中测定结果.以非参数法计算99%参考区间.结果 Troponin Ⅰ共385例,不按年龄和性别划分的参考区间上限值为0.01μg/L.CK-MB按年龄划分的参考区间上限值为:A组(7个月~1岁)128例:13.41μg/L;B组(2~3岁)128例:9.35 μg/L;C组(4~12岁)129例:5.48 μg/L.结论 确定了本院实验室健康儿童(7个月~12岁)Troponin Ⅰ和按年龄分组CK

  18. Correlation between level of serum prolactin and epileptic discharges of electroencephalogram from 24 to 36 hours after epileptic onset

    Institute of Scientific and Technical Information of China (English)

    Xiaowei Hu; Wanli Dong; Min Xu

    2006-01-01

    BACKGROUND: Researchers discovered that serum prolactin could rise following an epileptic seizure. The prolactin level might reach three times more than basic level within 30 minutes and decrease to the normal value 2 hours after the seizure occurred. The mechanism might result in an increase of serum prolactin concentrations with the activation of the hypothalamic-pituitary axis.OBJ ECTIVE:To probe into the correlation between changes of serum prolactin and incidence of epileptic discharges of electroencephalogram (EEG) at 24-36 hours after epileptic onset of patients with secondary epilepsy.DESIGN: Clinical observational study.SETTING: Department of Neurology, First Hospital affiliated to Soochow University.PARTICIPANTS: A total of 21 patients with secondary epilepsy were selected from the Department of Neurological Emergency or Hospital Room of the First Hospital affiliated to Soochow University from November 2005 to April 2006. There were 14 males and 7 females aged from 25 to 72 years. All patients met International League Anti-epileptic (ILAE) criteria in 1981 for secondary generalized tonic clonic seizure through CT or MRI and previous EEG. All patients were consent. Primary diseases included cerebral trauma (3 cases),tumor (2 cases), stroke (7 cases) and intracranial infeion (9 cases).METHODS: Venous blood of all patients was collected at 24-36 hours after epileptic onset. Serum prolactin kit (Beckman Coulter, Inc in USA) was used to measure value of serum prolactin according to kit instruction.Then, value of serum prolactin was compared with the normal value (male: 2.64-13.13 mg/L; female: 3.34-26.72 mg/L); meanwhile, EEG equipment (American Nicolet Incorporation) was used in this study.MAIN OUTCOME MEASURES: ① Abnormal rate of serum prolactin of patients with secondary epilepsy; ②Comparison between normal and abnormal level of serum prolactin and incidence of EEG epileptic discharge of patients with secondary epilepsy.RESULTS :All 21 patients with

  19. Statistics of assay validation in high throughput cell imaging of nuclear factor kappaB nuclear translocation.

    Science.gov (United States)

    Morelock, Maurice M; Hunter, Edward A; Moran, Timothy J; Heynen, Susanne; Laris, Casey; Thieleking, Michael; Akong, Michael; Mikic, Ivana; Callaway, Scott; DeLeon, Rodney P; Goodacre, Angela; Zacharias, David; Price, Jeffrey H

    2005-10-01

    This report describes statistical validation methods implemented on assay data for inhibition of subcellular redistribution of nuclear factor kappaB (NF kappaB) in HeLa cells. We quantified cellular inhibition of cytoplasmic-nuclear translocation of NF kappaB in response to a range of concentrations of interleukin-1 (IL-1) receptor antagonist in the presence of IL-1alpha using eight replicate rows in each four 96-well plates scanned five times on each of 2 days. Translocation was measured as the fractional localized intensity of the nucleus (FLIN), an implementation of our more general fractional localized intensity of the compartments (FLIC), which analyzes whole compartments in the context of the entire cell. The NF kappaB antagonist assay (inhibition of IL-1- induced NF kappaB translocation) data were collected on a Q3DM (San Diego, CA) EIDAQtrade mark 100 high throughput microscopy system. [In 2003, Q3DM was purchased by Beckman Coulter Inc. (Fullerton, CA), which released the IC 100 successor to the EIDAQ 100.] The generalized FLIC method is described along with two-point (minimum-maximum) and multiple point titration statistical methods. As a ratio of compartment intensities that tend to change proportionally, FLIN was resistant to photobleaching errors. Two-point minimum-maximum statistical analyses yielded the following: a Z' of 0.174 with the data as n = 320 independent well samples; Z' by row data in a range of 0.393-0.933, with a mean of 0.766; by-plate Z' data of 0.310, 0.443, 0.545, and 0.794; and by-plate means of columns Z' data of 0.879, 0.927, 0.945, and 0.963. The mean 50% inhibitory concentration (IC50) for IL-1 receptor antagonist over all experiments was 213 ng/ml. The combined IC50 coefficients of variation (CVs) were 0.74%, 0.85%, 2.09%, and 2.52% for the four plates. Repeatability IC50 CVs were as follows: day to day 3.0%, row to row 8.0%, plate to plate 2.8%, and day to day 0.6%. The number of cells required for statistically resolvable

  20. Digital seafloor images and sediment grain size from the mouth of the Columbia River, Oregon and Washington, 2014

    Science.gov (United States)

    Gelfenbaum, Guy R.; Carlson, Emily; Stevens, Andrew; Rubin, David M.

    2017-01-01

    Geo-referenced digital imagery of in-situ seafloor sediments in the mouth of the Columbia River was collected and analyzed to determine median grain size of the surface sediments. Digital imagery of the seafloor was collected with a “flying eyeball” (Rubin and others, 2007) from the R/V Parke Snavely from September 11 to September 13, 2014 (USGS Field Activity 2014-642-FA). The flying eyeball consists of a standard definition plumbing inspection video camera and LED light ring inserted in a 50 kg wrecking ball. The video camera has a resolution of 480 by 720 pixels with a resolution of 0.009 mm/pixel when the target is flush against the exterior surface of the lens. Sample locations were chosen to allow for the observation of spatial variability of grain size over the length of many large bedforms. The bedforms were identified from a bathymetric survey performed in a previous field effort in 2013 (Gelfenbaum and others, 2015). During survey operations, the flying eyeball was repeatedly lowered to the seafloor with a winch along the transect. The winch was equipped with a conducting cable that transmitted the video signal from the camera to the research vessel in real time where it was recorded with a Sony DV recorder. Positioning of the vessel was determined with an Applanix PosMV and integrated with the digital video recording at 1-Hz intervals using Red Hen Systems (RHS) VMS200 hardware, which encodes the position information on the audio channel of the video tape. In addition to the imagery, surface sediment was collected using a small Ponar, or "grab", sampler at 12 locations throughout the study area. The physical samples were emptied into a tray on the deck of the vessel and observed with the flying eyeball. Approximately 50 g of sediment from each sample was retained and later processed in the lab using a Beckman Coulter laser diffraction analyzer to determine grain-size distributions, which can be accessed on the "child item" to this data release

  1. Study on the relation between anemia and trace elements of infant%贫血与婴幼儿微量元素含量关系的研究

    Institute of Scientific and Technical Information of China (English)

    刘建雷; 曾赤佳; 赵东; 饶雪飞; 段穗萍

    2012-01-01

    OBJECTIVE To discuss how the anemia of infant affected the contents of the trace elements, and understand the relation between the anemia of infant and the trace elements. METHODS The blood were collected from the tip of the infants' finger, those whom takes part in the child hygiene. The hemoglobin was tested by the Beckman—Coulter company's Blood Cell Analyzer. Meanwhile, the contents of iron, calcium, zinc, magnesium, cuprum and plumbum of the wholeblood were tested by the Trace Element Determinator. According to the hemoglobin, the anemic was detected among infants, and non-anemic infants with the anemics were collected as control group. RESULTS The contents of iron and magnesium were patently sexual by random. We divided them into be two control groups, and the iron and magnesium in the anemic group were lower than those of the normal control (P< 0.01). The concentrations of the calcium and the lead were patently higher than the normal control, and the discrepancy was full of statistical significance as well (P< 0.01 and P< 0.05). But the zinc and the cuprum elements in the wholeblood did not show significant difference. CONCLUSION The anemic infant was frequently found to be IDA, the calcium , the magnesium and the plumbum elements did related to the anemia of infant.%目的 探讨婴幼儿贫血对其微量元素含量的的影响,了解婴幼儿贫血与微量元素的关系.方法 采集儿保要幼儿手指末梢血,采用Beckman-Coulter公司的血细胞分析仪检测血红蛋白含量,同时采用微量元素测定仪检测全血铁、钙、锌、镁、铜及铅元素含量.依据血红蛋白含量判断贫血婴幼儿,同时随机抽取与贫血婴幼儿年龄和性别比例接近的未贫血婴幼儿作为正常对照组,分析两组之间各微量元素结果.结果 贫血组婴幼儿全血铁元素和镁元素明显低于正常对照组,差异有统计学意义(均P<0.01);钙元素和铅元素浓度明显高于正常对照

  2. Novel Role of Merlin Tumor Suppressor in Autophagy and its Implication in Treating NF2-Associated Tumors

    Science.gov (United States)

    2012-04-01

    ORGANIZATION: Beckman Research Institute of the City of Hope Duarte , CA 91010-3012 REPORT DATE: April 2012 TYPE OF REPORT: Annual PREPARED...REPORT NUMBER Beckman Research Institute of the City of Hope Duarte , CA 91010-3012 9. SPONSORING / MONITORING AGENCY NAME(S...Southern California / House Ear Institute) has a collection of such cell lines, and we have initiated the collaborative work to address this issue

  3. Comparison of a new rapid plasma reagin card test with the standard rapid plasma reagin 18-mm circle card test and the venereal disease research laboratory slide test for serodiagnosis of syphilis.

    OpenAIRE

    1983-01-01

    The rapid plasma reagin (RPR) card test manufactured by Beckman Instruments, Inc., was compared, qualitatively and quantitatively, with the Venereal Disease Research Laboratory (VDRL) slide test and the standard RPR 18-mm circle card tests for the serodiagnosis of syphilis. Sera from 638 individuals were used in this study. Two pilot lots and two production lots of antigen were submitted by Beckman Instruments, Inc., for evaluation. Qualitative agreement among the three RPR card tests was 98....

  4. An Ecological Land Survey for Fort Wainwright, Alaska,

    Science.gov (United States)

    1999-09-01

    CERATOPHORUM (Ledeb.) DC. APOCYNTJM ANDROSAEMIFOLIUM L. TARAXACUM OFFICINALE G. Weber Araceae TRIPLEUROSPERMUM INODORUM (L.) Schultz-Bip. CALLA PALUSTRIS L...Coulter & Rose) Mathias & SOLIDAGO MULTIRADIATA Aiton Constance SONCHUS ARVENSIS L. SIUM SUAVE Walter SONCHUS ASPER (L.) Hill Apocynaceae TARAXACUM

  5. ECOHAB: Kamykowski_D- Florida Process Cruise 11/16-19/1998- Karenia brevis counts, biochemistry and behavior (NODC Accession 0000534)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Abstract: Water bottle samples were collected from selected depths (variable with station) at 14 stations in the study area. Coulter counts in the 14-28 um size...

  6. ECOHAB: Kamykowski_D- Florida Process Cruise 11/16-19/1998- Karenia brevis counts, biochemistry and behavior (NODC Accession 0000533)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Abstract: Water bottle samples were collected from selected depths (variable with station) at 44 stations in the study area. Coulter counts in the 14 to 28 um size...

  7. Effect of Replacement of New Reverse Osmosis Film of Water Purification System to Clinical Biochemistry%实验室纯水系统更换反渗透膜对生化检验的影响

    Institute of Scientific and Technical Information of China (English)

    莫基浩; 刘鲜茹; 李少侠

    2014-01-01

    目的:研究实验室纯水系统更换新反渗透膜后,所产纯水的质量是否符合生化检验的要求并评价其影响。方法通过Beckman Coulter生化分析系统配套纯水机天创TCHS-RO/100A自带电阻表和 HM COM-100电导率测量笔连续监测新产纯水中的实时离子含量。用正常纯水在 AU680上重复20次测量 ALT,AST,TBIL,ALP,GGT,TP,ALB,BUN, CR,UA,GLU,TC,TG,HDL,LDL,CK,LDH,P,Ca(对照组),更换为新产纯水后进行相同试验操作(试验组),以评估新产纯水对生化分析的影响。连续5天,每天3次(间隔2 h)监测新产纯水的 TG试剂空白,监测水中甘油水平。结果开机30 min后,HM COM-100测得电导率降至(0.1±0.0)μS/cm,机载电阻表测得新产纯水在开机50 min后稳定在18.25 MΩcm。在用 AU680进行生化分析时,对照组中TG测量值=1.04 mmol/L,试验组=21.39 mmol/L,差异有统计学意义(P<0.01),而除TG其他生化项目偏倚均在5%以内,CV<1/4CLIA’88,差异无统计学意义(P>0.05)。TG试剂空白在第5天稳定在A660nm=0.0156±0.0004。结论新纯水系统在生产约2800 L水后,所产纯水中的甘油可以清除干净。实验室纯水系统在更换反渗透膜后,需要对水质进行连续监测,以评价其对常规生化项目检测的影响。%Objective To test if the quality of produced water could come up to the standards of clinical biochemistry,and e-valuate the effect of the produced water after replacement of a new reverse osmosis film of the laboratory water purification system.Methods The ion concentration of the produced water was tested by ohmmeter in Beckman Coulter supporting TianChuang water purification system TCHS-RO/100A and HM COM-100 conductivity meter.The biochemical index ALT,AST,TBIL,ALP,GGT,TP,ALB,BUN,CR,UA,GLU,TC,TG,HDL,LDL,CK,LDH,P,Ca of mixed serum were tested 20 times by AU680 under the condition of

  8. The allergenic significance of certain fungi rarely reported as allergens.

    Science.gov (United States)

    Giannini, E H; Northey, W T; Leathers, C R

    1975-12-01

    The allergenic significance of seven different species of fungi was investigated. Included were Chlorophyllum molybdites, Podaxis pistillaris, Stemonitis ferruginea, Lycogala epidendrum, Fuligo septica, Ustilago maydis and Puccinia cynodontis. All of these fungi have wide distribution patterns and aerially disseminated spores but, because of their unique growth characteristics, are usually not reported in atmospheric fungal surveys. Seventy-eight patients were treated for dermal sensitivity to extracts of the organisms after the spores were extracted in 50% glycerinated Coca's solution. The results represent a six-month test period. Forty-four patients, representing 56% of the total number tested, demonstrated dermal reactivity toward one or more of the extracts.

  9. Myxomycetes em Palmeiras (Arecaceae Myxomycetes on palm trees (Arecaceae

    Directory of Open Access Journals (Sweden)

    Maria Helena Alves

    1996-07-01

    Full Text Available As palmeiras oferecem um ecossistema especializado que abriga organismos variados, incluindo Myxomycetes. Este trabalho relata doze espécies de Myxomycetes encontradas em folhas mortas, espata e estipe de Acrocomia intumescens Drude (macaúba, Copernicea prunifera (Mill. H. E. Moore (carnaúba, Mauritia flexuosa Mart, (buriti e Orbygnia phalerata Mart, (babaçu. Constitui-se área de coleta o Município do Crato, Ceará, Nordeste do Brasil (7º 30'00" S, 39º 00' 00" W, 400-1200 m de altitude. Coletas aleatórias foram efetuadas e as frutificações foram pesquisadas em órgãos vivos ou mortos das palmeiras. Exsicatas foram depositadas no Herbário UFP (Departamento de Botânica da Universidade Federal de Pernambuco, Recife - PE, Brasil. As espécies registradas pertencem aos gêneros Ceratiomyxa (1, Dictydium (1, Arcyria (1, Hemitrichia (1, Badhamia (1, Fuligo (1, Physarum (3, Stemonitis (2 e Comatricha (1. Ceratiomyxa fruticulosa (Miill. Macbr., Physarum stellatum (Mass. Mart., Stemonitis pallida Win. e Comatricha typhoides (Bull. Rost. são referidas pela primeira vez para este tipo de substrato. Este trabalho eleva para 35 o número de espécies de Myxomycetes assinaladas sobre palmeiras até o momento.Palm trees are specialized ecossystems which hosts a wide variety of organisms, including Myxomycetes. This paper reports twelve species of Myxomycetes found on dead leaves, spathes and stipitis of Acrocomia intumescens Drude, Copernicea prunifera (Mill. H. E. Moore, Mauritia flexuosa Mart., and Orbygnia phalerata Mart. The collecting area is located in the Municipality Crato of Ceará State, Northeastern Brazil (7º 30'00" S, 39º 00' 00" W, 400-1200 m altitude. Samples were taken at random and Myxomycetes fructifications were searched on dead and living organs of palm trees. Exsiccatae have been deposited in the Herbarium UFP (Department of Botany, Federal University of Pernambuco, Recife-PE, Brazil. The species registered belong to the

  10. Differential microfluidic sensor on printed circuit board for biological cells analysis.

    Science.gov (United States)

    Shi, Dongyuan; Guo, Jinhong; Chen, Liang; Xia, Chuncheng; Yu, Zhefeng; Ai, Ye; Li, Chang Ming; Kang, Yuejun; Wang, Zhiming

    2015-08-01

    Coulter principal based resistive pulse sensor has been demonstrated as an important platform in biological cell detection and enumeration since several decades ago. Recently, the miniaturized micro-Coulter counter has attracted much attention due to its advantages in point of care diagnostics for on chip detection and enumeration of rare cells, such as circulating tumor cells. In this paper, we present a microfluidic cytometer with differential amplifier based on Coulter principle on a SU-8 coated printed circuit board substrate. The electrical current changes induced by the blockage of the microparticles in the sensing aperture are calibrated by polystyrene particles of standard size. Finally, HeLa cells are used to evaluate the performance of the proposed device for enumeration of biological samples. The proposed cytometer is built upon the cheap and widely available printed circuit board substrate and shows its great potential as personalized healthcare monitor.

  11. Isolation and identification of the pathogens of potato fungus diseases during storage in Gansu Province%甘肃马铃薯贮藏期真菌性病害病原菌的分离鉴定

    Institute of Scientific and Technical Information of China (English)

    李金花; 柴兆祥; 王蒂; 李敏权

    2007-01-01

    对甘肃省马铃薯贮藏期真菌性病害的病原菌进行了分离鉴定,共鉴定到8种病原真菌,引起6种贮藏期真菌病害,分别为:致病疫霉(Phytophthom infestans)引起马铃薯晚疫病,茄链格孢(Alternaria solani)引起马铃薯早疫病,茄丝核菌(Rizoctonia solani)引起马铃薯黑痣病,具柄细基束梗孢(Cephalotrichum stemonitis)引起马铃薯褐腐病,Verticillium tricopsis引起马铃薯萎蔫病,Fusarium solani和F.sambucinum引起马铃薯于腐病.马铃薯为V.tricopsis的新寄主,具柄细基束梗孢(C.stemonitis)引起马铃薯褐腐病、F.sambucinum引起马铃薯干腐病在我国属首次报道.

  12. Initial responses of rove and ground beetles (Coleoptera, Staphylinidae, Carabidae to removal of logging residues following clearcut harvesting in the boreal forest of Quebec, Canada

    Directory of Open Access Journals (Sweden)

    Timothy Work

    2013-01-01

    Full Text Available Increased interest in biomass harvesting for bioenergetic applications has raised questions regarding the potential ecological consequences on forest biodiversity. Here we evaluate the initial changes in the abundance, species richness and community composition of rove (Staphylinidae and ground beetles (Carabidae, immediately following 1 stem-only harvesting (SOH, in which logging debris (i.e., tree tops and branches are retained on site, and 2 whole-tree harvesting (WTH, in which stems, tops and branches are removed in mature balsam fir stands in Quebec, Canada. Beetles were collected throughout the summer of 2011, one year following harvesting, using pitfall traps. Overall catch rates were greater in uncut forest (Control than either stem-only or whole-tree harvested sites. Catch rates in WTH were greater than SOH sites. Uncut stands were characterized primarily by five species: Atheta capsularis, A. klagesi, A. strigosula, Tachinus fumipennis/frigidus complex (Staphylinidae and to a lesser extent to Pterostichus punctatissimus (Carabidae. Increased catch rates in WTH sites, where post-harvest biomass was less, were attributable to increased catches of rove beetles Pseudopsis subulata, Quedius labradorensis and to a lesser extent Gabrius brevipennis. We were able to characterize differences in beetle assemblages between harvested and non-harvested plots as well as differences between whole tree (WTH and stem only (SOH harvested sites where logging residues had been removed or left following harvest. However, the overall assemblage response was largely a recapitulation of the responses of several abundant species.

  13. Symptoms and Pathogens of Nine Diseases of Ginseng%人参9种病害的症状和病原物

    Institute of Scientific and Technical Information of China (English)

    白容霖

    2002-01-01

    报道了人参的5种新病害:人参褐斑病(Acremoniella cucurbitae Schulz.et Sacc.)、人参黑腐病(Doratomyces stemonitis (Pers.et Fr.) Morton & Smith)、人参白粉病(Erysiphe panacis Bai et Wang)、人参褐腐病(Monilia cinerea Bon)、人参线虫病(Meloidogyne hapla Chitwood)和国内新记录的3种人参病害:人参灰霉病(Botrytis cinerea Pers.)、人参黑干腐病(Phoma panacicola Nakata et Takimoto)、人参粘菌病(Stemonitis herbatica Peck.).订正了吉林省人参细菌性软腐病的病原物,即为胡萝卜软腐欧文氏菌胡萝卜软腐亚种(Erwinia carotovora subsp. carotovora Dye)、胡萝卜欧文氏菌黑胫亚种(E.carotovora subsp. atroseptica Dye)、石竹假单胞菌(Pseudomonas caryophyll (Burkholder) Starr & Burkholder).

  14. Determination of renal clearances of amylase/creatinine with chromogenic and enzymatic methods.

    Science.gov (United States)

    Hohenwallner, W; Wimmer, E; Sommer, R

    1979-12-01

    Urinary amylase was estimated by chromogenic (amylochrome Roche) as well as enzymatic methods (SKI and Beckman: substrate starch and substrate maltotetraose respectively). Random and timed urines (24 hour collections) were analysed. Clearances of amylase gave different results dependent upon the amylase-test used and the glomerular filtration rate. Correlation between chromogenic and enzymatic methods (starch as substrate) was poor. The ratio of amylase and creatinine clearance was used to test different methods. Reference values for this ratio for the amylochrome method (N = 106) were 2.85 +/- 0.99% and for the Beckman-DS method (N = 60) 2.82 +/- 0.87%.

  15. 反相高效液相色谱紫外法检测人血清尿酸%Determination of uric acid in human serum by reverse-phase high-performance liquid chromatography using ultraviolet detection

    Institute of Scientific and Technical Information of China (English)

    江雅平; 刘欧; 徐国宾; 陈文祥; 张传宝; 张江涛

    2012-01-01

    with the mean values of the participating laboratories. The correlation coefficients between reverse-phase HPLC-UV and 5 routine assay systems, i. e. , Hitachi, Beckman Coulter, Roche, Dade and Vitros were 0. 998 9, 0. 996 5 , 0. 999 2, 0. 999 2 and 0.998 7 respectively. The deviations between of reverse-phase HPLC-UV and 5 routine assay systems were all less than biological variation (5% ). Conclusion The reverse-phase HPLC-UV developed in this study displayed as a simple, specific, rapid, precise and accurate method, and its results showed close correlation and consistency with routine traceable systems, so it can be recommended as a candidate reference method for determination of uric acid in human serum.

  16. Preparation of a Homologous (Human) Intravenous Botulinal Immune Globulin.

    Science.gov (United States)

    1983-05-01

    spectrophotometer, Beckman "Instruments) is determined. A four point standard curve is constructed using bovine serum albumin (Cat. #81-016, Miles Laboratories...silica dioxide 25Prekatikrein 94’ 0m..hr (SiO2) treatmen! of plasma before fractionation on ’ Kaolin &ZOVAI.ed QAE, and (2) thle elution pH of the QAE

  17. The Effects of Husband’s At-Sea Time upon the Role Playing Behavior of the Submariner’s Wife

    Science.gov (United States)

    1978-08-30

    Ombudsmen, and wives of senior enlisted personnel were in- vited to attend the briefing and question and answer period. I! U; 24 BIBLIOGRAPHY Bateson ... Gregory 1972 Culture Contact and Schismogenesis. In Steps to an Ecology of Mind. Pp. 61-72. New York: Ballantine Books (1935). Beckman, Karen __ 1976

  18. Conformal Sheets of Thin Film Sensors, Electronics and Energy Harvesters for Structural Monitoring

    Science.gov (United States)

    2012-08-01

    Science and Engineering, Electrical and Computer Engineering, Bioengineering Mechanical Science and Engineering, and Chemistry Beckman Institute for...neurology D D C ll (P ) di lr. . a ans enn – car o ogy Dr. M. Slepian (Sarver) – cardiology Dr. J. McDonald (JHU) – rehabilitation Dr. I. Efimov (Wash Univ) -- cardiology

  19. The STRONG STAR Multidisciplinary PTSD Research Consortium

    Science.gov (United States)

    2009-09-01

    two 6 ml of ice cold incubation buffer, air dried, then placed in scintillation vials with 8 ml of Beckman Redi - Solv scintillation fluid for counting...Zungu-Dirwayi N, et al (2002) Pharmacotherapy for Posttraumatic Stress Disorder. The Cochrane Database of Systematic Reviews (4). Tarrier N, Pilgrim H

  20. Role of Extracellular miR-122 in Breast Cancer Metastasis

    Science.gov (United States)

    2015-02-01

    Metastasis” PRINCIPAL INVESTIGATOR: Miranda Fong CONTRACTING ORGANIZATION: Beckman Research Institute of the City of Hope Duarte, CA 91010... CA 91010 9. SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S ACRONYM(S) U.S...if alternation of glucose metabolism in the pre-metastatic niche contributes to circulating tumor cell colonization and metastatic formation. 4

  1. Course Management Systems and Blended Learning: An Innovative Learning Approach

    Science.gov (United States)

    Chou, Amy Y.; Chou, David C.

    2011-01-01

    This article utilizes Rogers' innovation-decision process model (2003) and Beckman and Berry's innovation process model (2007) to create an innovative learning map that illustrates three learning methods (i.e., face-to-face learning, online learning, and blended learning) in two types of innovation (i.e., incremental innovation and radical…

  2. Organizational Perceptions of Telecommuting in the Private Sector

    Science.gov (United States)

    Galusha, Repps J.

    2011-01-01

    The Internet has provided more organizations the opportunity to adopt telecommuting as a means to recruit and retain employees, boost productivity, and trim facility costs. This study expands on the work of a previous study by Hoang, Nickerson, Beckman, and Eng, in 2008 which found that private organizations, due to perceptions of organizational…

  3. The quadratic assignment problem is easy for Robinsonian matrices with Toeplitz structure

    NARCIS (Netherlands)

    Laurent, M.; Seminaroti, M.

    2014-01-01

    We present a new polynomially solvable case of the Quadratic Assignment Problem in Koopmans-Beckman form QAP(A,B), by showing that the identity permutation is optimal when A and B are respectively a Robinson similarity and dissimilarity matrix and one of A or B is a Toeplitz matrix. A Robinson (dis)

  4. The SDF-1-CXCR4 Axis Functions Through p38-MAPK Signaling to Drive Breast Cancer Progression and Metastasis

    Science.gov (United States)

    2009-09-01

    2002). Rubin JB, Kung AL, Klein RS, Chan JA, Sun Y, Schmidt K, Kieran MW, Luster AD, Segal RA.A small-molecule antagonist of CXCR4 inhibits... Melanie H. Howell, Michelle Lacey, Betty Y. Shih, Carol Carter-Wientjes, Thomas E. Cleveland, John A. McLachlan, Thomas E. Wiese, Barbara S. Beckman

  5. Determination of spectrophotometric absorptivity by analytical ultracentrifugation

    Directory of Open Access Journals (Sweden)

    M Senthilraja

    2011-01-01

    Full Text Available Rapid determination of the absorptivity for a recombinant IgG monoclonal antibody using the Beckman equipped with both Raleigh interference and UV absorbance optical systems. The analytical ultracentrifuge data for determining spectrophotometric absorptivities is compared to experimental data from quantitative amino acid analysis and an enzymatic digestion method.

  6. Evaluation of Biological and Male Reproductive Function Responses to Potential Lead Exposures in 155 mm Howitzer Crewmen

    Science.gov (United States)

    1992-01-01

    VARICOSE VEINS OF THE SCROTUM (Varicocele)? 0 1 9 (41) d. CIRCUMCISION? 0 1 9 (42) e. A FORESKIN THAT CAN’T BE PULLED BACK (Phimosis)? 0 1 9 (43) f...0.1 ml using a plastic syringe. The pH was measured within 90 minutes of ejaculation using a gel filled plastic pH probe (Beckman Instruments

  7. The Quadratic Assignment Problem is Easy for Robinsonian Matrices

    NARCIS (Netherlands)

    Laurent, M.; Seminaroti, M.

    2014-01-01

    We present a new polynomially solvable case of the Quadratic Assignment Problem in Koopmans-Beckman form QAP(A;B), by showing that the identity permutation is optimal when A and B are respectively a Robinson similarity and dissimilarity matrix and one of A or B is a Toeplitz matrix. A Robinson (dis)

  8. The quadratic assignment problem is easy for robinsonian matrices

    NARCIS (Netherlands)

    Laurent, M.; Seminaroti, M.

    2015-01-01

    We present a new polynomially solvable case of the Quadratic Assignment Problem in Koopmans–Beckman form QAP(A,B), by showing that the identity permutation is optimal when AA and BB are respectively a Robinson similarity and dissimilarity matrix and one of AA or BB is a Toeplitz matrix. A Robinson (

  9. Muscle Stem Cell Therapy for the Treatment of DMD Associated Cardiomyopathy

    Science.gov (United States)

    2014-10-01

    typical fat stores underneath skin , and has been reported to be associated with aging, inactivity, obesity, and diseases like diabetes (10, 14-15). Fatty... Antioxidation and Immunomodulation in Postnatal Multipotent Stem Cell-Mediated Cardiac Repair. Saparov A, Chen C-W, Beckman SA, Wang Y, Huard J

  10. 不同地区招飞体检学生静脉血细胞参数对比分析%Comparison on the venous blood cells parameters of the candidates in physical examination for pilot recruitment in different areas

    Institute of Scientific and Technical Information of China (English)

    厉淑红; 郑军; 周金立; 马新伟

    2015-01-01

    Objective To analyze the difference and variation rule of the parameters of venous blood cells in the pilot candidates from different areas,and provide a reference for the revision of physical examination standard and physical evaluation.Methods The candidates were divided into seven groups:East China,South China,Central China,North China,Northwest China,Southwest China,and Northeast China,according to the areas they came from,and 224 candidates were randomly selected from each group.2 ml morning fasting venous blood was collected into a dipotassium ethylene tetraacetate anticoagulation tube.As the sample was mixed homogenously,parameters of blood cells were determined by Beckman Coulter system 2 Automated Hematology Analyzer and the results were shown in the form of mean ± standard deviation (x±s).The statistical analysis was performed by using one-way analysis of variance (ANOVA),and it took P<0.05 as the statistical significance of difference.Results Comparing the blood cell parameters of the candidates from different areas,WBC,neutrophilic granulocyte,lymphocyte,and monocyte showed no statistical significance (P > 0.05);eosinophilic granulocyte and basophilic granulocyte showed statistical significance (F=7.906,2.524,P<0.01);all parameters of red blood cell and platelet showed statistical significance (F=4.435-14.864,F=4.847-20.657,P<0.01).The percentage of abnormality was respectively 18,11% in RBC,43.56% in Hb,14.16% in hemoglobin,17.54% in lymphocyte,9.06% in neutrophilic granulocyte,and 1.40% in platelet.Conclusions There are regional differences in venous blood cell parameters among the pilot candidates from different areas.It is necessary to improve the items of blood cell test and the evaluation standard considering the area difference in order to obtain more accurate physical evaluation and the references for the tracking survey of the blood cell parameters in pilots.%目的 分析我国不同地区招飞体检学生静脉血细

  11. Analysis of fractal magnitude of soil particles in loamy Chao soils in North China Plain%华北冲积平原壤质潮土的土壤粒径分形空间尺度分析

    Institute of Scientific and Technical Information of China (English)

    李晓鹏; 刘建立; 张佳宝; 王伟鹏; 辛文文

    2014-01-01

    It is widely accepted that soils have fractal characteristics in certain spatial scales. However, the exact magnitude of this spatial self-similarity is often neglected when doing fractal related researches. In this study, 43 sets of soil particle size distribution data of the Chao soil in North China Alluvial Plain were collected, and their particle size distributions with 93 levels were determined by laser diffraction analysis method (Beckman Coulter LS 13-320).The spatial magnitude orders of soils can be taken as fractal objectives and their affecting factors were analyzed based on the particle size distribution data. For determining the fractal magnitude of the soils, we used a typical scale method:1) calculate the differential fractal dimensions in all the 93 particle size levels (Di, i=1, 2,…, 93) according to the definition fractal; 2) choose a typical scale which is supposed to follow self-similarity (however, the measured Di values may still fluctuate in a certain range); 3) suppose Di in the typical scale is normally distributed, and calculate the corresponding distribution characteristics; 4) use the distribution characteristics to find out the lower and upper limits of Di which follows the same normal distribution in the whole range from the lower to upper limit (in the study, 0.5%and 99.5%of the accumulation curve were taken as the lower and upper limits, respectively);5) fit Di in the entire fractal magnitude, and the slope is supposed to be the fractal dimension of the soil.The results showed that the Chao soils in the study area mostly had good gradations, and they only had rigorous fractal structure at small to medium particle sizes (large clay to fine silt), but didn’t show spatial self-similarity in coarse sand scale. Soils with more clay particles usually had larger fractal dimensions, and the extents of fractal reduced with the increasing of clay fraction. Soils with good graduations usually had wider fractal extents. The fractal dimension

  12. 血清总IgE和外周血嗜酸性粒细胞在特应性皮炎患者检测中的临床意义%Clinical significance of total serum IgE and peripheral blood eosinophil in detection of atopic dermatitis

    Institute of Scientific and Technical Information of China (English)

    许志萍

    2013-01-01

    Objective To investigate the clinical significance of total serum IgE and peripheral blood eosino-phils (EOS) in detection of atopic derma (AD)and the relationship between total serum IgE ,EOS and AD .Methods 40 cases of AD patients(observation group) and 40 normal cases (control group) in Guangzhou the First People's Hospital from May 2010 to January 2012 were randomly selected .The HITACHI 7170A automatic biochemical tester and the BECKMAN COULTER GEN .S automatic blood cell analyzer were used to detect the total serum IgE and peripheral blood EOS concentration in two groups .Comparison and analysis of the differences between IgE and EOS concentration of two groups and their correlation with AD .Results The total serum IgE and EOS concentration of the observation group were (1 256 .53 ± 1 036 .84) U/mL and (569 .46 ± 322 .13) × 106/L respectively ,which were significantly higher than that of control group (P<0 .05) .The correlation coefficient OR value between IgE and EOS and susceptibility of AD reached 4 .402 and 3 .753 respectively ,the difference was statistically significant (P<0 .05) . There was a significant positive correlation between the degree of AD and IgE and EOS and AD prevalence (P<0 .05) and the correlation coefficient reached 0 .878 and 0 .833 ;the correlation coefficient between IgE and EOS reached 0 .790 ,which showed significant positive correlation (P<0 .05) .Conclusion There is correlation between total serum IgE ,EOS and ocurrence and disease activity in AD ,can provide a basis for AD clinical diagnosis ,therapy and curative effect evaluation .%目的探讨血清总IgE和外周血嗜酸性粒细胞(EOS)与特应性皮炎(AD)的关系及其在AD检测中的临床意义。方法随机选择2010年5月至2012年1月,在广东省广州市第一人民医院接受治疗的AD患者40例(观察组)和健康体检者40例(健康对照组),分别采用日立7170A全自动生化检测仪和贝克曼库尔特GEN .S全自动血

  13. Association study of WNT3A gene polymorphisms with the susceptibility to congenital scoliosis in a Chinese Han population%中国汉族人群WNT3A基因多态性与先天性脊柱侧凸遗传易感性的关系

    Institute of Scientific and Technical Information of China (English)

    费琦; 吴志宏; 王以朋; 周熹; 王海; 王乃国; 李想; 邱贵兴

    2011-01-01

    gender.Genomic DNA was extracted by QIAamp DNA Blood Mini Kit from peripheral blood leukocytes of each subject who had signed informed consent.Based on the genotypic data from the International HapMap project,the main functional single nucleotide polymorphisms (SNPs) were initially selected.The patients in the case group were classified into different clinical phenotypes according to vertebral defect type,location of deformity,extent of developmental disruption,combined rib malformations and neural canal deformity.The genotying of all selected SNPs was performed by SNPstream technology (Beckman Coulter SNPstream).All data of SNPs with polymorphism were processed by the association analysis based on a single SNP and between phenotypes and SNPs.And the pairwise linkage disequilibrium was calculated in the control population by Haploview 4.1 software.Results The SNP1 (rs964941) and SNP2(rs752107) of WNT3A were genotyped.There was no linkage disequilibrium between two SNPs.No association was observed between SNP1 and SNP2 genotypes or allele polymorphisms and risk of CS and various clinical phenotypes (P > 0.05).Conclusions The genetic variants of WNT3A gene may not be associated with the susceptibility to CS and various clinical phenotypes of CS in Chinese Han population.

  14. 中国汉族人群SIM2基因多态性与先天性脊柱侧凸遗传易感性的关联研究%Association study of SIM2 gene polymorphisms with susceptibility to congenital scoliosis in a Chinese Han population

    Institute of Scientific and Technical Information of China (English)

    费琦; 吴志宏; 周熹; 王海; 王乃国; 尹若峰; 王以朋; 邱贵兴

    2009-01-01

    age 12.90 y/o)diagnosed with CS admitted at Peking Union Medical College(PUMC)Hospital were enrolled between October 2005 and September 2007.The scoliosis-free control subjects(127 cases)at the same hospital during the same study period were frequency-matched to the cases on age(±3 years)and gender.Genomic DNA was extracted by QIAamp DNA Blood Mini Kit from peripheral blood leukocytes of each subject who had signed informed consent.Based on genotype data from the International HapMap project,the main functional single nucleotide polymorphisms(SNPs)initially were selected.Case group were classified into different clinical phenotypes according to vertebral defect type,location of deformity,extent of developmental disruption,combined rib malformations and neural canal deformity.Genotying of all selected SNPs was done by SNPstream technology (Beckman Coulter SNPstream).All the data of SNPs with polymorphism were analyzed by association analysis based on a single SNP.the association analysis between phenotypes and SNPs.And pairwise linkage disequilibrium was calculated in the control population using Haploview 4.1 software.Results SNPI (rs2073601),SNP2(rs2073417)and SNP3(rs2051397)of SIM2 are genotyped.SNP2 and SNP3 in linkage disequilibrium.No association(P>0.05)is observed between SNP1,SNP2 and SNP3 genotypes/allele polymorphisms and risk of CS and different clinical phenotypes.Conclusion Genetic variants of SIM2 gene may not be associated with the susceptibility to CS and different clinical phenotypes of CS in Chinese Han population.

  15. Drug and Vaccine evaluation in the Human Aotus Plasmodium falciparum Model

    Science.gov (United States)

    2011-05-01

    BUN) using a Coulter Counter hematological analyzer and a Reflotron dry chemistry machine. On day 0, 8, 12, 15 and 19 PI when the CBC is done, if an...HCT % less than 50% of baseline a decision will be made by the attending veterinarian to continue or remove the animal from the experiment and treat

  16. 78 FR 68464 - Performance Review Board

    Science.gov (United States)

    2013-11-14

    ..., Jemine A. Bryon, Stanley A. Gimont, John P. Benison, David R. Ziaya, Damon Y. Smith, Mary K. Kinney, Charles S. Coulter, Jean Lin Pao, Ricky T. Valentine, and Mary E. McBride as members of the Departmental... toll-free number.) Dated: November 7, 2013. Maurice A. Jones, Deputy Secretary. BILLING CODE 4210-67-P...

  17. Pikantsed pajatused / Aita Kivi

    Index Scriptorium Estoniae

    Kivi, Aita, 1954-

    2000-01-01

    Sisu : Louis Paul Boon. Menuett; Herbjırg Wassmo. Õnne poeg; Jean Stone. Sünnipäevatüdrukud; Jo︠l Schmidt. Constantinus Suure mälestused; Mari Vallisoo. Ainsuse olevik; Catherine Coulter. Märklaud; Ross Macdonald. Mees mulla all

  18. "Response to Comments": Finding the Narrative in Narrative Research

    Science.gov (United States)

    Coulter, Cathy A.

    2009-01-01

    The author responds to comments by Barone (2009), Clandinin and Murphy (2009), and M. W. Smith (2009) on "The Construction Zone: Literary Elements in Narrative Research" (Coulter & M. L. Smith, 2009). She clarifies issues regarding point of view, authorial surplus, narrative coherence, and the relational qualities of narrative research. She…

  19. 2x Ben Affleck / Laurence Lumiere

    Index Scriptorium Estoniae

    Lumiere, Laurence

    2007-01-01

    Põnevusfilm "Hollywoodland" : režissöör Allen Coulter : Ameerika Ühendriigid 2006 ja põnevuskomöödia "Äss mättasse" ("Smokin' Aces") : režissöör Joe Carnahan : Suurbritannia - Prantsusmaa - Ameerika Ühendriigid 2007. Mõlemas mängib Ben Affleck

  20. 77 FR 75947 - Endangered and Threatened Wildlife and Plants; Reclassification of the Continental U.S. Breeding...

    Science.gov (United States)

    2012-12-26

    ... population of the wood stork (USFWS 1997), The Birds of North America Online species account for wood stork..., amphibians, reptiles, mammals, birds, and arthropods (Coulter et al. 1999, p. 7). Wood storks forage in a... The wood stork occurs in South America from northern Argentina, eastern Peru, and western...

  1. Optimization, Application, and Interpretation of Lactate Dehydrogenase Measurements in Microwell Determination of Cell Number and Toxicity

    NARCIS (Netherlands)

    Wolterbeek, H.T.; Van der Meer, A.J.G.M.

    2005-01-01

    The lactate dehydrogenase (LDH) assay was addressed for its sensitivity, disturbances by foaming, and cell number and size. Cells were from a U-251 MG grade IV human glioblastoma brain tumor cell line used in 100-µl well volumes. Cells were counted by microscopy and Coulter counting; assays were LDH

  2. Effects of injection systems and plastic mulches on distribution and emissions of cis- and trans-1,3-dichloropropene and chloropicrin.

    Science.gov (United States)

    Ou, Li-Tse; Thomas, John E; Allen, L Hartwell; Vu, Joseph C; Dickson, Donald W

    2007-08-01

    The concentration and distribution of a soil fumigant in the subsurface of field plots are two key factors in the determination of the fumigant efficacy. Subsurface concentrations of the biologically active compounds cis- and trans-1,3-dichloropropene (1,3-D) and chloropicrin (CP) were determined in soil at two adjacent injection traces and midpoint between the two traces in plastic-covered field beds after injection of the fumigant Telone C35 by conventional chisels or by a coulter rig (Avenger coulters). Two of the four beds were covered with metallic polyethylene film (MPE) and the remaining two were covered with virtually impermeable film (VIF). Three hours after chisel injection, concentrations of the three compounds at the two adjacent injection traces in the two beds were highly variable. Large concentrations of the compounds were detected at the side traces, whereas the compounds were not detected at the middle traces (bed centers) in the two chisel-injected beds covered with MPE or VIF. Initial concentrations of the three compounds at the two adjacent injection traces in the two Avenger-coulter-injected beds were more uniform than in the chisel-injected beds but still variable. Also, the three compounds had diffused horizontally to midpoint between the two injection traces in all four beds, generally in smaller concentrations. Concentrations of the three compounds in the shallow subsurface soil layer (0.5-30 cm) in the beds became progressively more uniform and then declined slowly. Volatilization losses from the two Avenger-coulter-injected beds and the chisel-injected bed covered with VIF were low. Combination of Avenger coulter injection and VIF provided better retention of the compounds in this soil layer and, thus, likely would provide better fumigant efficacy and crop yield.

  3. Diversity of plasmodial slime molds (myxomycetes in coastal, mountain, and community forests of Puerto Galera, Oriental Mindoro, the Philippines

    Directory of Open Access Journals (Sweden)

    Nikki Heherson A. Dagamac

    2015-12-01

    Full Text Available No profiling of diversity of myxomycetes has ever been conducted in one of the biodiversity hotspot areas in the Philippine archipelago, and this necessitates a swift survey of myxomycetes in Puerto Galera, Oriental Mindoro. An assessment of diversity of myxomycetes collected from seven collecting points of three different forest types in the study area showed a total of 926 records of myxomycetes. Of which, 42 morphospecies belonging to 16 genera are reported in this study. Species richness of myxomycetes was higher in collecting points that were found in inland lowland mountain forests, but the most taxonomically diverse species was found in coastal forests. Myxomycete species, namely, Arcyria cinerea, Diderma hemisphaericum, Physarum echinosporum, Lamproderma scintillans, and Stemonitis fusca, were found in all the collecting points. Manmade disturbances and forest structure may affect the occurrence of myxomycetes.

  4. Assessing soil calcium depletion following growth and harvesting of Sitka spruce plantation forestry in the acid sensitive Welsh uplands

    Directory of Open Access Journals (Sweden)

    B. Reynolds

    1998-01-01

    Full Text Available A simple mass balance has been used to estimate soil calcium depletion during the growth of a 50 year old Sitka spruce crop on acid, base-poor peaty podzol soils in upland Wales. Growth of the crop will deplete the soil calcium reserve by an amount (205 kg Ca ha-1 approximately equivalent to the exchangeable calcium pool to the bottom of the profile and equal to 14% of the total soil calcium reserve to the bottom of the B horizon. Despite these predictions, measurements of exchangeable calcium show no differences beneath mature forest and acid grassland, implying that i weathering rates in forest soils are greater than long-term estimates and predictions by the PROFILE soil chemistry model ii the trees can access other sources of calcium or iii there are significant errors in the mass balance. Following stem-only harvesting, growth of a 50 year old second rotation crop will lead to further depletion of soil calcium, but this amount (79 kg Ca ha-1, is less than for a second rotation crop following whole-tree harvesting (197 kg Ca ha-1. After the first crop, stem-only harvesting would allow a further 18 rotations before depletion of the total calcium reserve to the bottom of the B horizon. Whole-tree harvesting would allow for seven rotations after the first crop. These calculations assume that all sources of calcium are equally available to the crop. This can only be resolved by dynamic modelling of the calcium cycle at the ecosystem scale based on appropriate field measurements. The potential for significant soil acidification is therefore greater following whole-tree harvesting and, in line with current recommendations (Nisbet et al., 1997, this technique should probably be avoided on acidic, nutrient-poor soils unless remedial measures are included to enhance the soil base cation status.

  5. N cycling and the composition of terpenes and tannins in boreal forest soils: Effects of logging residues

    Science.gov (United States)

    Smolander, Aino; Kitunen, Veikko; Kukkola, Mikko; Tamminen, Pekka

    2014-05-01

    There is increasing evidence available that certain terpenes and tannins may mediate substantial changes in nitrogen cycling processes in boreal forest soils. Terpenes and tannins are two important groups of plant secondary metabolites: Terpenes are hydrocarbons having different number of isoprene-derived units and tannins are complex polyphenolic compounds able to interact with proteins. Logging residues, consisting of fresh tree tops and branches with needles contain large amounts of terpenes and tannins. Currently there is increasing demand for forest biomass for bioenergy production. Therefore, harvesting of logging residues has become more common from both clear-cutting and thinning stands, instead of conventional stem-only harvest where logging residues are retained on the site. Our aim was to determine how logging residues affect soil N cycling processes in Scots pine and Norway spruce thinning stands in long-term, and how these processes are related to the composition of terpenes and tannins in the soil. Samples were taken from the humus layer of pine and spruce experiments which had been thinned 4-to-19 years before; in the thinning different amounts of logging residues had been distributed on the plots. Logging residues had only little effect on soil microbial biomass N or C. However, in several sites logging residues increased the rate of net N mineralization and the ratios net N mineralization/ C mineralization and net N mineralization/microbial biomass N, and these positive effects were very long-lasting. Logging residues also changed the composition of different terpenes and condensed tannins in soil. In general, with regard to the processes and ratios indicating N availability, stem-only harvest seems to be more favorable than whole-tree harvest. The results from long-term field experiments will be discussed in relation to the effects of different terpenes and tannins, observed in short-term laboratory experiments, on N cycling processes.

  6. Student Perceptions of Motivational Behaviors of Instructions in a Military Setting

    Science.gov (United States)

    2005-06-01

    motivational behaviors, then students will be motivated to learn. Social learning theory (Bandura, 1977) emphasizes the importance of observing and...information serves as a guide for action. ( p. 22) Social learning theory explains the behavior of teacher and students in terms of continuous mutual...methods (2nd ed.). Belmont, CA: Wadsworth Publishing Company. Bandura, A. (1977). Social learning theory . New York: General Learning Press. Beckman, T

  7. A Drug Discovery Partnership for Personalized Breast Cancer Therapy

    Science.gov (United States)

    2014-09-01

    Tropical Medicine, “LINEs and SINEs differ in their retrotransposition requirements and cellular interactions” 6- Monday November 18, 2013, Dr. Anup...Cheryl K. Stevens and Jayalakshmi Sridhar- review article submitted to the journal ‘Molecules’, Aug. 2014. “Functionalization and Modification... pediatrics at the University of North Carolina.) Barbara Drew, Medical Student at Tulane University (Beckman Lab, is currently in her residency in

  8. Department of Defense In-House RDT and E Activities: Management Analysis Report for Fiscal Year 1993

    Science.gov (United States)

    1994-11-01

    portable light boxes. rhe isolation facility also includes a treadmill for exercising subjects. Equipment: I Polysomnography : Three Beckman (SessorMedics...daily monitoring by Navy planners, will use these data to update frorecasts of future gains and losses, will reevaluate accession plans in light of the...Upgrade to ARIA; GSP/JNS Upgrade to ARIA; ARIA Air Refueling Upgrade; F-15 lHardwe System Trainer (HST); Combat Talon Aircraft Updates ; Halon Replacement

  9. P53 Gene Mutagenesis in Breast Cancer

    Science.gov (United States)

    2005-03-01

    Research Institute, Duarte, California 2Department of Molecular Diagnosis , City of Hope National Medical Center and Beckman Research Institute, Duarte...Director, Depart- posure risk. ments of Molecular Genetics and Molecular Diagnosis , City of 1-lope Mutation load has been studied in rodents using...Steve S. Sommer, Chair, Departments of Molecular et al., 1994]. Striking evidence for the geographic-specific Genetics and Molecular Diagnosis , Professor

  10. Sexual Assault and Sexual Harassment in the U.S. Military: Volume 2. Estimates for Department of Defense Service Members from the 2014 RAND Military Workplace Study

    Science.gov (United States)

    2016-01-01

    Burkhart, M.S. Robin Beckman, M.P.H. Megan Mathews, M.A. Marc Elliott, Ph.D. Project Management Kayla M. Williams, M.A. Caroline Epley, M.P.A. Amy Grace...discrimination in the military, but with these new details come new questions that will require additional research. Top priorities for future...instrument. The leadership and staff in each of the services’ sexual assault prevention and response offices provided many rounds of review

  11. Role of Extracellular miR-122 in Breast Cancer Metastasis

    Science.gov (United States)

    2016-02-01

    Hope Beckman Research Institute, Duarte, California 91010, USA. 2Department of Biotherapy and Key Laboratory of Cancer Immunology , Tianjin Medical...1 AWARD NUMBER: W81XWH-14-1-0029 TITLE: “Role of Extracellular miR-122 in Breast Cancer Metastasis” PRINCIPAL INVESTIGATOR: Miranda Fong...the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this

  12. Joint Services Electronics Program.

    Science.gov (United States)

    1985-03-30

    Dr. Paul Sasm (USACECOM) and Dr. Jeff Wieselthier (NRL). In addition, our work on coding and diversity has been influenced by considerations arising...Investigator Award, 1984 Beckman Asociate, Center for Advanced Study, UIUC, 1984-85 Eckman Award, American Automatic Control Council Xerox Award for Faculty...appear. (Lockheed) 9. W. K. Jenkins, D. F. Paul , and E. S. Davidson, ’A custom-designed integrated circuit for the reali- zation of residue number digital

  13. The Construct of State-Level Suspicion A Model and Research Agenda for Automated and Information Technology (IT) Contexts

    Science.gov (United States)

    2013-08-01

    2008) and branding alliances (Lowry et al., 2008) Willingness of persons in an electronic social system to provide personal information (Ridings, Gefen...Lowry, P., Vance, A., Moody, G., Beckman, B., & Read, A. (2008). Explaining and Predicting the Impact of Branding Alliances and Web Site Quality...Responsibility ( CSR ) Activities on Companies with Bad Reputations. Journal of Consumer Psychology, 16, 377-390.   32 Distribution A. Approved for

  14. Mathematical Modeling and Optimal Control of Battlefield Information Flow

    Science.gov (United States)

    2008-06-01

    touched upon in this research: the family of Quadratic Assignment Problems ( QAPs ) and the area of sequencing and scheduling. We shall address each of...these in this literature review. 1. Quadratic Assignment Problem Koopmans and Beckman [14] first introduced the Quadratic Assignment Problem ( QAP ...in 1957 as a mathematical model for the assignment of n “indivisible economic activities” (i.e., plants) to n locations. The general QAP is known to

  15. 应用CLSI文件对化学发光免疫分析系统性能的初步评价%Preliminary Evaluation of the Performance of the Chemiluminescent Immunoassay System by CLSI File

    Institute of Scientific and Technical Information of China (English)

    张帮林; 郭永灿

    2014-01-01

    目的:评价BECKMAN ACCESS2全自动微粒子化学发光免疫分析系统相关性能。方法:以测定游离三碘甲状腺原氨酸(FT3)为例,收集患者高、中、低值新鲜血清混合标本,应用CLSI颁布的EP10-A2对ACCESS2全自动化学发光免疫分析系统进行初步评价。结果:通过CLSI EP10-A2程序评价显示ACCESS2检测系统偏差、不精密度、交叉污染、线性漂移等因素都在可接受范围内,对精密度无明显影响。结论:ACCESS2全自动发光免疫分析系统分析性能可靠,适合临床实验室使用。%Objective:To evaluate the performance of Beckman Access2 automated chemiluminescent immunoassay system in order to know its status. Method:Using the CLSI document EP10-2A for preliminary evaluation methods to approve the high,medium and low fresh serum of patients which were adopted as samples and was assayed FT3 in Beckman Access2 testing system.Result:The factors of carryover and drift,system deviation,imprecision,cross contamination in the ballpark,they had no effect on precision.Conclusion:The performance of Beckman Access2 automated chemiluminescent system is reliable and suitable for clinical laboratory to use.

  16. On the Calibration of a Size-Structured Population Model from Experimental Data

    CERN Document Server

    Jauffret, Marie Doumic; Zubelli, Jorge P

    2009-01-01

    The aim of this work is twofold. First, we survey the techniques developed in (Perthame, Zubelli, 2007) and (Doumic, Perthame, Zubelli, 2008) to reconstruct the division (birth) rate from the cell volume distribution data in certain structured population models. Secondly, we implement such techniques on experimental cell volume distributions available in the literature so as to validate the theoretical and numerical results. As a proof of concept, we use the data reported in the classical work of Kubitschek [3] concerning Escherichia coli in vitro experiments measured by means of a Coulter transducer-multichannel analyzer system (Coulter Electronics, Inc., Hialeah, Fla, USA.) Despite the rather old measurement technology, the reconstructed division rates still display potentially useful biological features.

  17. 长时间高原训练对优秀赛艇运动员免疫机能及血浆瘦素的影响%Effect of Long-term Altitude Training on the Immunity and Plasma Leptin of Elite Rowers

    Institute of Scientific and Technical Information of China (English)

    高欢; 高炳宏; 冯连世; 孟志军

    2011-01-01

    二者之间的关系.方法:20名优秀男子赛艇运动员进行持续60天的高原训练(海拔2200m),训练分为两个大的训练阶段,7天为1个训练周,每个训练周包含2个小周期.内容以中低强度有氧耐力训练为主.上高原后第1、4、10、25、38、60天,即每个训练小周期、训练周或大训练阶段调整结束后次日清晨,空腹采集前臂静脉血.酶联免疫吸附法测定血清瘦素,化学发光仪测定血清皮质醇,以Beckman COULTER ACT10血球仪测定白细胞及其分类计数,以Binding Site AD200光散射光度计测定免疫球蛋白.结果:(1)与上高原前的(6.55±1.31)×109/L比较,WBC计数在上高原后1天[(5.62±2.26)×109/L]下降14.2%,高原第10天为(6.51±0.97)×109/L,回升至接近高原前水平;高原第25天为(5.24±0.82)×109/L,与高原前、高原第10天比较显著下降(P<0.05). (2)LY%与WBC计数变化趋势近似,高原第25天[(29.37±5.88)]%大幅下降,与高原前的(40.43±6.64)%和高原第10天的(36.62±6.94)%比较有显著性差异(P<0.05);高原训练第38天、第60天LY%分别为(32.49±4.92)%、(28.18±3.71)%,均显著低于高原前.(3)上高原1天后,血清IgG[(12.11±2.18)g/L]显著升高(P<0.05);之后,IgG无明显变化;血清IgM和IgA高原训练阶段与高原前比较均无显著改变. (4)整个高原训练过程中,血清LP均值无显著性改变(变化范围6.12~6.56 ng/ml).结论:高原训练第25天左右,免疫机能可能下降;经10天调整训练后略有恢复,至上高原后第60天仍低于高原前.长时间高原训练过程中,血清LP与免疫机能之间的变化关系仍需研究.

  18. LMX1A基因多态性与中国汉族人群先天性脊柱侧凸遗传易感性的关联%Association of LMX1A gene polymorphisms with susceptibility to congenital scoliosis in a Chinese Han population

    Institute of Scientific and Technical Information of China (English)

    费琦; 吴志宏; 王以朋; 周熹; 王海; 李想; 邱贵兴

    2011-01-01

    BACKGROUND: Congenital scoliosis (CS) is caused by the abnormal development of embryonic spinal vertebrae, and its geneticetiology hypothesis has begun to attract the attention of many scholars.OBJECTIVE: To investigate whether polymorphisms of LMX1A gene are associated with CS in a Chinese Han population, andfurther, to study the relationship between polymorphisms of LMX1A and the clinical phenotypes of CS.METHODS: A total of 127 patients diagnosed with CS and the scoliosis-free control subjects (127 cases) were enrolled in thisstudy. Genomic Based on genotype data from the International HapMap project, the key single nucleotide polymorphisms (SNPs)initially were selected using Haploview 4.1 software. Hardy-Weinberg equilibrium both in control and in case groups was analyzed.Case group were classified into different clinical phenotypes according to vertebral defect type, the location of deformity, the extentof developmental disruption, combined rib malformations and neural canal deformity. Genotying of all selected SNPs was done bySNPstream technology (Beckman Coulter SNPstream). All the data of SNPs with polymorphism are analyzed by the associationanalysis based on a single SNP. And pairwise linkage disequilibrium wa s calculated in the control population using Haploview 4.1software.RESULTS AND CONCLUSION: Six SNPs of LMX1A gene (SNP1: rs1819768, SNP2: rs12023709, SNP3: rs16841013, SNP4:rs4656435, SNP5: rs4657412, SNP6: rs4657411) were genotyped and all polymorphisms were in Hardy-Weinberg equilibriumboth in control and in case groups. The single locus analysis revealed the genotype distributions of SNP1 and SNP2 werestatistically significantly different between case patients and control subjects (P=0.026 and P=0.026). In the unconditional logisticregression analysis, SNP1 and SNP2 both showed significant difference in both Ressessive model (OR=0.38; 95%CI=0.15-0.94)and overdominant model (OR=1.73; 95%CI=1.05-2.8), and the P values were 0.029 and 0

  19. High molecule compound synthesis from carbon dioxide; Nisanka tanso karano kobunshi gosei

    Energy Technology Data Exchange (ETDEWEB)

    Mishima, K. [Fukuoka Univ., Fukuoka (Japan)

    2000-09-05

    Recently, synthesis of organic substances and high molecule compounds using carbon dioxide of liquid or supercritical state as raw material is paid attention so as the influential means to realize the green chemistry. A zinc based catalyst which could synthesize the activated copolymer from oxidized cyclohexane and carbon dioxide was developed by Coates, Beckman et al. The high-activated fluoridation zinc based catalyst which dissolved the high-pressure carbon dioxide was developed by Beckman, etc. The possibility of the manufacturing of the high molecule copolymer which is made from carbon dioxide and oxidized cyclohexane as a raw material was shown by an appearance of this catalyst. In addition, a synthesizing method of lactone from carbon dioxide and olefinic hydrocarbon is being examined by Beckman. If this method is used practically, the manufacturing of a gentle biodegradable polymer in the environment which is made from carbon dioxide as a raw material becomes also possible. However, adequate catalysts are necessary so that this reaction may progress. (NEDO)

  20. Systematic Review of Integrative Health Care Research: Randomized Control Trials, Clinical Controlled Trials, and Meta-Analysis

    Science.gov (United States)

    2010-01-01

    Khorsan,1, 2 Ian D. Coulter,3, 4, 5 Cindy Crawford,6 and An-Fu Hsiao7, 8 1Military Medical Research and Integrative Medicine, Samueli Institute, 2101...PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Samueli Institute,Military Medical Research and Integrative Medicine,2101 East Coast Hwy,Corona Del...study. This paper is supported by the US Army Medical Research and Materiel Command under Award no. W81XWH-07-2-0076 through the Samueli Institute

  1. Anticoagulant-induced pseudothrombocytopenia and pseudoleucocytosis.

    Science.gov (United States)

    Schrezenmeier, H; Müller, H; Gunsilius, E; Heimpel, H; Seifried, E

    1995-03-01

    Pseudothrombocytopenia (PTP) is the phenomenon of falsely low platelet counts due to in vitro platelet clumping in the presence of platelet autoantibodies and anticoagulants. We assessed anticoagulant-dependence, time course of platelet counts and impact of different counter devices on the phenomenon. Blood of 10 persons with previously recognized pronounced EDTA-dependent PTP was collected into 7 different anticoagulants and counted after different intervals in parallel in a Coulter T540 and a Coulter STKS counter and by phase contrast microscopy. With the Coulter T540 model PTP was most pronounced in blood samples anticoagulated with EDTA, Na-oxalate or Na-citrate. In the STKS counter EDTA, heparin and oxalate presented as the worst anticoagulants. The time course of platelet counts was significantly different between the two counters. Our results demonstrate that PTP is not restricted to EDTA, but is also present with other anticoagulants. In contrast, pseudoleucocytosis was observed only in EDTA-anticoagulated blood in the Coulter T540 device. We investigated the expression of platelet integrins and activation antigens on platelets of persons with anticoagulant-dependent PTP and in healthy controls without PTP. In the presence of EDTA the expression of GpIIb/IIIa was significantly reduced in the PTP subjects compared to control. Activation antigens CD62, CD63 and thrombospondin-antigen were upregulated in the presence of EDTA. These alterations in the expression of platelet antigens could also be induced on platelets of normal donors by incubation with sera of PTP subjects and EDTA.(ABSTRACT TRUNCATED AT 250 WORDS)

  2. Chemical and Biological Characterization of Black Rock Harbor Dredged Material.

    Science.gov (United States)

    1985-09-01

    the first test (Table.- 833). Dissolved oxygen was at or above saturation. Mortality in the control appeared to be the result of gas supersaturation ...Reference Strain and Four Geographical Strains of Artemia as Food for Winter Flounder (Pseudopleuronectes americanus) Larvae," Aquaculture , Vol 29, pp...EVIDENCE OF SUPERSATURATION . (1) PERCENT FOR SOLID PHASE TESTS (2) MILLIGRAMS/LITER FOR SUSPENDED PARTICULATE TESTS, DRY WEIGHTS OR COULTER COUNTS OR

  3. SEI Report on Graduate Software Engineering Education for 1991

    Science.gov (United States)

    1991-04-01

    September 1989 Contact Neal Coulter Department of Computer Science Florida Atlantic University PO Box 3091 Boca Raton, FL 33431-0991 Source This...and pos - CMU/SEI-91-TR-2 123 sibly user representatives, external reviewers, and others. Then the walkthrough is scheduled, an agenda prepared, and...Assessment Reading: NASA86b How are post- mortems , histories, lessons-learned documents, etc. useful? What makes an organization reluctant to share lessons

  4. Plasma Volume during Heat Stress and Exercise in Women,

    Science.gov (United States)

    1986-11-01

    subjectively determined "thermoneutral" environment. Methods Five healthy women (Table 1), who were not using oral contraceptive agents, volunteered to...with no difference between phases. Blood volume was estimated by the method of Allen et al (1) using the weight of the subject measured during the...a hemoglobinometer (Coulter Electronics). Plasma protein concentration (Pp) was measured by refractometry . Plasma sodium (Na + ) and potassium (K

  5. Gastric Emptying During Exercise: Effects of Acute Heat Stress, Acclimation and Hypohydration,

    Science.gov (United States)

    1987-10-01

    rehydration proceedures to be employed by athletes, workers and military personnel engaged in physical activity. Methods Subjects. Ten males volunteered for...ingested and the process repeated with each subject completing a total of three i5 min exercise bouts per experiment. The reliability of this method for...analyzed in triplicate for hemoglobin (Hemoglobinometer, Coulter Electronics), hematocrit (micro-centrifugation), plasma protein ( refractometry , American

  6. Annual Conference on Human Retrovirus Testing (7th) held in Chicago, IL on March 3-5, 1992

    Science.gov (United States)

    1993-04-05

    Annual Conference on Human Retrovirus Testing 15 TESIING : PANEL~ SESSION IIITESTNG-NON-SEROLOGIC TESTING MET~HODS PANEL CHAIR: Haynes W (Chip) Sheppard...Illinois Coulter Corporation Department of Public Health Myrtam Garcia Negron, Medical Technolog~st, Bryan Peterson. Ph.D.. Abbott Laboratories Puerto ...NC State Laboratory of Public Health Puerto Rico Department of Health Alfred Saah. M.D., Associate Professor of prema M. Singa. M.D.. Director

  7. Myxomycetes on carnaubeira (Copernicia prunifera (Miller H. E. Moore, Arecaceae Myxomycetes em carnaubeira (Copernicia prunifera (Miller H. E. Moore, Arecaceae

    Directory of Open Access Journals (Sweden)

    Mitra Mobin

    2000-04-01

    Full Text Available Entre dezembro/1994 e setembro/1995 foram realizadas sete excursões (2 a 4 dias cada ao Parque Nacional de Sete Cidades (4º5'-4º15'S, 41º30'-41º40'W, 450m alt., em Piripiri, Piauí, visando coletar frutificações de Myxomycetes em indivíduos de Coperniciaprunifera (Miller H. E. Moore, estabelecidos em área de cerrado, na localidade Lagoa Seca. As amostras foram herborizadas e incorporadas ao herbário UFP (Universidade Federal de Pernambuco, Recife, PE, com duplicatas no herbário TEPB (Universidade Federal do Piauí, Teresina, PI. Registrou-se apreciável incidência (70% de Myxomycetes na carnaubeira, em estipe vivo e folhas mortas. Dezesseis espécies, pertencentes às Ceratiomyxaceae (Ceratiomyxa lsp., Didymiaceae (Diachea lsp., Didymium 2spp., Physaraceae (Craterium 1sp., Physarum 4spp., Trichiaceae (Arcyria 2spp. e Stemonitaceae (Comatricha 3spp., Macbrideola lsp., Stemonitis lsp. foram registradas. Ceratiomyxaceae, representada por Ceratiomyxafruticulosa (Mull. Macbr., constitui primeira referência para o Estado do Piauí.From December, 1994 to September, 1995, seven collecting trips (2-4 days each were carried out in the "Parque Nacional de Sete Cidades" (4º5'-4º15'S, 41º30"-41º40'W, 450m alt., Piripiri, Piauí State, Brazil, in order to collect Myxomycetes fruiting bodies on carnauba palm trees (Coperniciaprunifera (Miller H. E. Moore, established in a place called "Lagoa Seca" (savanna-like stunted vegetation. The samples were herborized and included in the UFP Herbarium (Universidade Federal de Pernambuco, Recife, PE and the TEPB Herbarium (Universidade Federal do Piauí, Teresina, PI. In relation to the substrate, the "carnaubeira" showed a high incidence of Myxomycetes (70% on living stipitis and dead leaves. Sixteen species belonging to Ceratiomyxaceae (Ceratiomyxa lsp., Didymiaceae (Diachea lsp., Didymium 2spp., Physaraceae (Craterium lsp., Physarum 4spp., Trichiaceae (Arcyria 2spp. and Stemonitaceae (Comatricha 3spp

  8. DIVERSITY AND DISTRIBUTION OF PLASMODIAL MYXOMYCETES (SLIME MOLDS FROM LA MESA ECOPARK, QUEZON CITY, PHILIPPINES

    Directory of Open Access Journals (Sweden)

    THOMAS EDISON E. DELA CRUZ

    2010-10-01

    Full Text Available Myxomycetes are ubiquitous in terrestrial forest ecosystems. Thus, this research study looks at the taxonomic diversity and distribution of plasmodial myxomycetes in La Mesa Ecopark in Quezon City, Philippines. A total of 240 moist chambers were prepared from four substrates (aerial and ground leaf litter, twigs and barks collected within this ecopark. Following incubation of moist chambers for eight weeks, a total of 28 species belonging to 10 genera were collected and identified: Arcyria (3, Diderma (2, Didymium (5, Lamproderma (2, Perichaena (3, Physarum (8, Macbrideola (1, Metatrichia (1, Trichia (1 and Stemonitis (2. Highest myxomycete yield (85% was observed in aerial leaf litter. In terms of taxonomic diversity, highest diversity was observed in bark microhabitats, although the lowest number of species was recorded in it. Assessment of their abundance and distribution showed similarities in species composition between aerial and ground leaf litter. This research study is the first report of plasmodial myxomycetes in La Mesa Ecopark in Quezon City, Philippines.

  9. Invalidation of Hyperamoeba by transferring its species to other genera of Myxogastria.

    Science.gov (United States)

    Fiore-Donno, Anna Maria; Kamono, Akiko; Chao, Ema E; Fukui, Manabu; Cavalier-Smith, Thomas

    2010-01-01

    The genus Hyperamoeba Alexeieff, 1923 was established to accommodate an aerobic amoeba exhibiting three life stages-amoeba, flagellate, and cyst. As more species/strains were isolated, it became increasingly evident from small subunit (SSU) gene phylogenies and ultrastructure that Hyperamoeba is polyphyletic and its species occupy different positions within the class Myxogastria. To pinpoint Hyperamoeba strains within other myxogastrid genera we aligned numerous myxogastrid sequences: whole small subunit ribosomal (SSU or 18S rRNA) gene for 50 dark-spored (i.e. Stemonitida and Physarida) Myxogastria (including a new "Hyperamoeba"/Didymium sequence) and a approximately 400-bp SSU fragment for 147 isolates assigned to 10 genera of the order Physarida. Phylogenetic analyses show unambiguously that the type species Hyperamoeba flagellata is a Physarum (Physarum flagellatum comb. nov.) as it nests among other Physarum species as robust sister to Physarum didermoides. Our trees also allow the following allocations: five Hyperamoeba strains to the genus Stemonitis; Hyperamoeba dachnaya, Pseudodidymium cryptomastigophorum, and three other Hyperamoeba strains to the genus Didymium; and two further Hyperamoeba strains to the family Physaridae. We therefore abandon the polyphyletic and redundant genus Hyperamoeba. We discuss the implications for the ecology and evolution of Myxogastria, whose amoeboflagellates are more widespread than previous inventories supposed, being now found in freshwater and even marine environments.

  10. Co-existence and interaction between myxomycetes and other organisms in shared niches

    Directory of Open Access Journals (Sweden)

    Irina O. Dudka

    2013-12-01

    Full Text Available The ecology of myxomycetes co-existing with the Latridiidae (Coleoptera, bryophytes and ascomycetous, basidiomycetous and anamorphic fungi were studied in Crimea and at different locations on the left bank of Ukraine. Results from the left bank indicate that the Latridiidae feed on myxomycetes. Colonies of the most common 13 myxomycete species (which included Stemonitis axifera (Bull. Macbr., S. fusca Roth, S. splendens Rost., Fuligo septica (L. Wigg. and Mucilago crustacea Wigg. were inhabited by 5 species of the Latridiidae. Myxomycete spores were present in guts of 19 of the 25 beetle specimens investigated. Beetles Latridius hirtus, Enicmus rugosus and E fungicola seem to be obligate myxomycete feeders, while Corticarina truncatella was clearly facultative. 13 species of myxomycetes were recorded on 9 species of moss and 3 species of liverwort developing on decaying wood or bark in the Crimean Nature Reserve. Relations between myxomycetes and bryophytes on woody substrata are spatial rather than trophic, and are possibly regulated by specific microclimatic conditions inside bryophyte thallomes. 69 species of myxomycetes were found co-existing with 36 species of ascomycetes, 21 species of basidiomycetes and 9 species of anamorphic fungi in the Crimean Nature Reserve. Associations formed by myxomycetes and fungi on different woody substrata are analyzed.

  11. Critical loads and nitrogen availability under deposition and harvest scenarios for conifer forests in Ireland.

    Science.gov (United States)

    Johnson, James; Cummins, Thomas; Aherne, Julian

    2016-01-15

    In this study we calculated the critical load of nutrient nitrogen (N) for Irish forest plots (n=380) under two harvesting scenarios: conventional stem-only harvest (SOH) and stem plus branch harvest (SBH) and two deposition scenarios: current and with a 10% increase in reduced-N. In addition, current N status was assessed using the following data from forest monitoring plots: forest floor C:N, foliar N and plant root simulation (PRS™) probe N supply rate. Average critical loads were 15.3 kg N ha(-1)year(-1) under SOH and 19.5 kg N ha(-1)year(-1) under SBH. Average total (wet+dry) N deposition was 18 kg N ha(-1)year(-1), ranging from 8.6 to 26 kg Nha(-1)year(-1). As a result, critical loads were exceeded at 67% of sites under SOH and 40% of sites under SBH. However, there was little evidence of exceedance at monitored plots. Foliar and forest floor C:N data indicated that most of these sites had low to intermediate N status. There were considerable differences in N cycling between soil types. Plant root simulation (PRS™) probe data indicated that this was likely due to differences in net N-mineralization and nitrification. Our results indicate that many sites are currently N limited but critical load exceedance suggests that these systems will accumulate N over time. The findings have implications for forest management, allowing for the assessment of nutrient management under different harvest scenarios.

  12. Transformer Specification Language: A System for Generating Analyzers and Its Applications

    Science.gov (United States)

    2011-01-01

    vector arithmetic—in its solver. Quoting A. Nori , “[[98] handles] the complexities of binaries via its front-end Vulcan and not via its property...Components and Objects, 2005. [49] N.E. Beckman, A.V. Nori , S.K. Rajamani, and R.J. Simmons. Proofs from tests. In Int. Symp. on Softw. Testing and...M.Y. Levin, and D. Molnar. Automated whitebox fuzz testing. In Network and Dist. Syst. Security, 2008. [96] P. Godefroid, A.V. Nori , S.K. Rajamani, and

  13. The Vietnam Petroleum Industry: Positioned for Growth but Ripe for Regional Complications

    Science.gov (United States)

    2013-10-30

    2013, JSTOR . 17 “U.S. EIA, “Vietnam” 20 18 Trong Vu and Simon Hall, “Oil Market Changes as Vietnam Build Another Refinery,” The Wall...Survey 52, no. 6 (November/December 2012): 995-996. Accessed August 30, 2013. JSTOR . 28 U.S. EIA, “South China Sea” 29 Ibid. 30 Marc...30, 2013. JSTOR . 35 U.S. EIA, “South China Sea” 21 36 Robert Beckman, “The UN Convention on the Law of the Sea and the Maritime

  14. Auditory and Visual Evoked Potentials as a Function of Sleep Deprivation and Irregular Sleep

    Science.gov (United States)

    1989-08-15

    comparisons are made between Block 1 and Block 5; amplitude decreased from 11.3 uV on Block 1 to 5.4 uV on Block 5. Statistical analysis revealed that...trial analysis with various ERP waves ( Birch , Lawrence, & Hare, 1988; Chase, McCarthy, Squires, & Schaneveldt, 1984; Fabiani, Gratton, Karis, & Donchin...Beckman silver - silver chloride electrodes. The EEG signals were amplified and filtered by Grass 7P122 D.C. amplifiers. For EEG signals, the time

  15. Measurement of urinary constituents with the Astra-8 Multichannel Analyzer.

    Science.gov (United States)

    Blumenfeld, T A; Griffith, B

    1980-12-01

    The Beckman Astra-8 is a computerized, discrete-microsample, multiple-test clinical chemistry analyzer. We have examined its capability to measure urine Na+, K+, Cl-, urea nitrogen, glucose, and creatinine and found that (a) the Astra measurements are linear, accurate, and precise in concentration ranges of urine analytes; (b) urine analyte concentrations measured with the Astra correlate well with those by comparison micromethods (p < 0.001); and (c) the Astra has no significant carryover from adjacent urine samples or from adjacent urine and serum samples.

  16. Evaluation of a new multichannel analyzer, "Astra-8".

    Science.gov (United States)

    Finley, P R; Williams, R J; Lichti, D A; Thies, A C

    1978-12-01

    We evaluated a new multiple-channel chemistry analyzer, the Beckman "Astra-8," which is controlled by a microprocessor with 52K bytes of memory. The instrument we tested performed the following tests: Na, K, CO2, chloride, urea nitrogen, glucose, and creatinine. We compared the Astra-8 to a continuous-flow (SMA 6/60) and a discrete (Du Pont aca, urea nitrogen only) analyzer. The Astra-8 demonstrated excellent precision, linearity, accuracy, analytical recovery, lack of interference, ease of operation, and satisfactory comparison to values obtained by the comparison methods.

  17. Compatibility and stability of potassium chloride and magnesium sulfate in 0.9% sodium chloride injection and 5% dextrose injeciton solutions.

    Science.gov (United States)

    Quay, I; Tan, E

    2001-01-01

    The compatibility and stability of 80 mmol/L potassium chloride and 16 mmol/L magnesium sulfate in 0.9% sodium chloride injection and in 5% dextrose injection solutions at 22 deg C have been studied by means of a Beckman Clinical Chemistry Analyzer Synchron CX5 Delta. The infusions were stable for 24 hours at 22 deg C. The results from both diluents showed an average of +/-5% fluctuations in concentration. None of the samples appeared to form visible precipitation or to change in color or clarity.

  18. Determination of average molecular weights on organic reactor coolants. I.- Freezing-point depression method for benzene solutions; Determinaciond e masas moleculares medias en refrigerantes nucleares organicos. I.- Crioscopia de disolucion bencenicas

    Energy Technology Data Exchange (ETDEWEB)

    Carreira, M.

    1965-07-01

    As a working method for determination of changes in molecular mass that may occur by irradiation (pyrolytic-radiolytic decomposition) of polyphenyl reactor coolants, a cryoscopic technique has been developed which associated the basic simplicity of Beckman's method with some experimental refinements taken out of the equilibrium methods. A total of 18 runs were made on samples of napthalene, biphenyl, and the commercial mixtures OM-2 (Progil) and Santowax-R (Monsanto), with an average deviation from the theoretical molecular mass of 0.6%. (Author) 7 refs.

  19. Determination of average molecular weights on organic reactor coolants. II.-Freezing point depression method for diphenyl-ether solutions; Determinacion de masas moleculares medias en refrigerantes nucleares organicos. II.- Crioscopia de disoluciones en eter difenilico

    Energy Technology Data Exchange (ETDEWEB)

    Carreira, M.

    1965-07-01

    In order to reduce limitations of solubility, the cryoscopic method developed for benzene solutions of polyphenyl mixtures has been extended to diphenyl-ether solutions by introducing some modifications imposed by the physico-chemical properties of this solvent. The Nernsto theory of Beckman's method has been revised, taking into account the heat-transfer characteristics of the system, and the results of that analysis have been used to fix upon the design parameters of a cryoscopic apparatus for measurements on diphenyl-ether solutions. (Author) 9 refs.

  20. Carbon Slurry Fuels for Volume Limited Missiles

    Science.gov (United States)

    1979-11-01

    nge being 20 mole percent. The oxygen analyzer is a Beckman Model 776 and .... a maximum range of 25 mole percent. The gas chromatograph is a Carle ...salt can also have an important effec.t on the case of Kcarbon oxidation. For example, Nebel and t.ramer 54 ive a relative lictivity series for lead...1978. 54. Nebel , G. .J, and Cramer, P. L., Ind. Eng. Chem 47m 2393, 1955. - 231 - I APPENDIX C - PREPARATION OF HANSEN PLOTS (1) The first step is

  1. Cardiovascular alterations caused by the administration of 2% mepivacaine HCl with 1:20,000 levonordefrin (Carbocain) in dogs.

    Science.gov (United States)

    Simone, J L; Tortamano, N; Armonia, P L; Rocha, R G

    1997-01-01

    We studied possible cardiovascular effects (systolic, diastolic, mean arterial blood pressures, and heart rate) caused by intraoral infiltrative administration of 2% mepivacaine HCl with 1:20,000 levonordefrin in dogs (Canis familiaris), using a Beckman electrophysiograph. Doses used were 0.514 and 1.542 mg/kg body weight corresponding to one and three 1.8-ml cartridges, respectively, in 70-kg average weight adult men. A statistically significant increase was observed in the systolic and the mean arterial blood pressures.

  2. Toxic Hazards Research Unit Annual Report: 1986

    Science.gov (United States)

    1987-04-01

    the work effort sponsored by the Naval Medical Research and Development Command was under the direction of CAPT David E. Uddin, MSC, USN, and...133-1 R-1061-3b a David Taylor Naval Ship Research & Development Center. b Polyalphaolefin stock of sample B85-174. A Beckman Acculab 4 was used to...ENGINEERING D.W. Brade During this first year of NSI-ES’s operation of the THRU, a major effort of facilities engineering has been the development of

  3. Preclincal Studies of the Oxime, HI-6, an Element of the Treatment of Soman Poisoning. Appendix 13. The Pharmacokinetics of HI-6 in the Rat and Dog

    Science.gov (United States)

    1982-03-31

    shown that the H oximes are unstable in strongly alkaline solutions. Therefore buffers have been used to maintain a pH which ensures a suitable...clean test tube containing 4 ml of 0.05 M tris buffer pH 8.8g The samples were mixed as before and centrifuged to * 4Fisher Scientific Co., Fair Lawn...Thomas Company, Philadelphia, PA. I8 * 8Acta III, Beckman Instruments Inc., Fullerton, CA, 92634. 9 ’ Trizma 8.8, Sigma Chemical Co., St. Louis, MO 63178

  4. Stratigraphy and Glacial-Marine Sediments of the Amerasian Basin, Central Arctic Ocean.

    Science.gov (United States)

    1981-02-01

    particles and conducting an electronic si/C analisis of the material that passed through the sieve A, using a I A 11 Coulter counter. I he (oulter...Ocean mechanists. Normal pelagic and glacially dericd fine matelital are glacial-marine sediment should he viewed as a complex process distributed...meatn prain si/(i \\;ei iiade heisseri iis ri r it( f xindil atrca fac sedimencrt I\\. PCs at h Ii 05 0-1irf~ifr I. live) 11it11%~ iisr I tii basic

  5. Studies of a Bulbospinal Pathway that Regulates Cardiovascular Function: Inhibition by GABA at the Ventral Medulla and Mediation by Spinal Cord Substance P

    Science.gov (United States)

    1984-12-13

    t . Lou i s , MO) desmethyl imipramine ( g i f t from Revlon Health Care Group, Tuckahoe, NY) dexamethasone (Schering V e t e r i n a r y , Ken i...Localization of the central cardiovascular action of clonidine. _B_r. Ĵ. Pharmac. 49:573-579. Bowker, R.M.; Westlund, K.N; Sullivan, M.C.; Wilber ...Brain Res. 288:33-48. Bowker, R.M.; Westlund, K.N.; Sullivan, M.C.; Wilber , J.F. and Coulter, J.D. (1982a) Transmitters of the raphe-spinal complex

  6. Proofs of two conjectures on ternary weakly regular bent functions

    OpenAIRE

    Helleseth, Tor; Hollmann, Henk D. L.; Kholosha, Alexander; Wang, Zeying; Xiang, Qing

    2008-01-01

    We study ternary monomial functions of the form $f(x)=\\Tr_n(ax^d)$, where $x\\in \\Ff_{3^n}$ and $\\Tr_n: \\Ff_{3^n}\\to \\Ff_3$ is the absolute trace function. Using a lemma of Hou \\cite{hou}, Stickelberger's theorem on Gauss sums, and certain ternary weight inequalities, we show that certain ternary monomial functions arising from \\cite{hk1} are weakly regular bent, settling a conjecture of Helleseth and Kholosha \\cite{hk1}. We also prove that the Coulter-Matthews bent functions are weakly regular.

  7. A plate reader-based method for cell water permeability measurement

    DEFF Research Database (Denmark)

    Fenton, Robert A.; Moeller, H B; Nielsen, S

    2010-01-01

    Cell volume and water permeability measurements in cultured mammalian cells are typically conducted under a light microscope. Many of the employed approaches are time consuming and not applicable to a study of confluent epithelial cell monolayers. We present here an adaptation of a calcein-quenching-based......-mannitol concentrations. Similarly, according average cell volumes have been measured in suspension in a Coulter counter (particle-sizing device). Based on these measurements, we have derived an equation that facilitates the modeling of cell volume changes based on fluorescence intensity changes. We have utilized...

  8. Crystallization Thermodynamic and Kinetic Behaviors of Vitamin C in Batch Crystallizer

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The bench-scale cooling crystallization for ternary solution of L-ascorbic acid (Vitamin C) was studied. The solid-liquid phase diagram of Vitamin C-water-ethanol system was obtained on the basis of differential scanning calorimeter (DSC) curves. The heat of crystallization of Vitamin C was calculated with the aid of quantitative analysis. According to the population balance equation under unsteady state, the rates of nucleation and growth were determined. The parameters of crystallization kinetics equations were estimated by regression of experimental data. Crystal morphology and size were determined with x-ray diffraction and TA Ⅱ Coulter Counter.

  9. Patrones ferrocin??ticos en la beta-talasemia minor

    OpenAIRE

    P??rez Blanco, Francisco Jos??; Jim??nez Jim??nez, M. J.; Mart??n Fern??ndez, A.

    2014-01-01

    Objetivos: Estudiar los par??metros del metabolismo del hierro en la beta-talasemia minor. Casu??stica y M??todos: Se estudian 58 sujetos (30 hombre y 28 mujeres) de edades entre 18-72 a??os diagnosticados de talasemia beta minor. Como controles se estudiaron 30 hombres y 28 mujeres del mismo espectro de edad. El estudio hematol??gico se realiz?? en el Coulter Counter, el bioqu??mico en el autoanalizador Hitachi, la hemoglobina A2 mediante electroforesis en celogel. El hierro m...

  10. Advanced Concepts Theory Annual Report, 1991

    Science.gov (United States)

    1992-04-27

    experiments, and the development of a transmission line model with which to study power flow in TES machines, POSIPRS interactions, and energy coupling to PRS...the 11 Q. 30 2c calculation and - 2600 eV in the 2 - 9c calculation. In addition, when the same method used to determine the experimental temperature...time averaged temperature was 800 eV, but the method of Coulter et al13 predicted a temperature of 560 eV. Fig. 13 also shows that the peak electron

  11. Proxies and measurement techinques for mineral dust in antarctic ice cores

    DEFF Research Database (Denmark)

    Ruth..[], Urs; Bigler, Matthias

    2008-01-01

    To improve quantitative interpretation of ice core aeolian dust records, a systematic methodological comparison was made. This involved methods for water-insoluble particle counting (Coulter counter and laser-sensing particle detector), soluble ion analysis (ion chromatography and continuous flow...... analysis), elemental analysis (inductively coupled plasma mass spectroscopy at pH 1 and after full acid digestion), and water-insoluble elemental analysis (proton induced X-ray emission). Antarctic ice core samples covering the last deglaciation from the EPICA Dome C (EDC) and the EPICA Dronning Maud Land...

  12. Contornos de entonación en el discurso de una docente. Su relación con algunos aspectos pragmáticos

    Directory of Open Access Journals (Sweden)

    Guadalupe Álvarez

    2005-06-01

    Full Text Available En este artículo se describen los contornos de los grupos entonacionales del discurso de una docente con el objeto de determinar las características que adoptan en relación con algunos aspectos pragmáticos relevantes de ese discurso, como partículas de iniciación, continuidad, completud y diferentes actos de habla (aseveraciones, órdenes, pedidos o sugerencias y, sobre todo, distintas clases de preguntas. Para ello, realizo una descripción perceptual y físico-acústica de 172 grupos entonacionales combinando el sistema TOBI de notación (Beckman y Ayers Elam, 1993 con la marcación de valores ERB y la posición silábica (Gurlekian y otros, 1999. (The aim of this article is describe the contours of the intonational groups of a teacher’s discourse in order to determine the characteristics that they adopt in relation to some relevant pragmatic aspects of it, included initial particles, continuity, completion and different speech acts. To achieve this, I describe 172 intonational groups from an auditory and physical-acoustic analyses, combining the TOBI system notation (Beckman y Ayers Elam, 1993 with the ERB-rate scale and the syllabic position (Gurlekian et al, 1999.

  13. Determinação espectrofotométrica de cálcio em solos Spectrophotometric determination of calcium in soils

    Directory of Open Access Journals (Sweden)

    Fernando A. Soares Coelho

    1969-01-01

    Full Text Available É apresentado estudo da determinação de cálcio em extratos de solo por espectrofotometria de chama, utilizando o espectrofotômetro Beckman, modelo DU. A composição da chama, as pressões dos gases, as interferências e os métodos para eliminá-las foram estudados. Verificou-se ser imprescindível a eliminação das interferências causadas pelos íons de alumínio, ferro e fosfato nas concentrações que normalmente ocorrem nos solos. A utilização de EDTA a pH 9 apresentou resultados satisfatórios na eliminação dessas interferências quando em concentração 0,01 M na solução em que foi determinado o cálcio.A study is presented on the determination of calcium in soil extracts by flame photometry, using a Beckman Model DU spectrophotometer. Flame composition, gas pressure, interferences and their elimination were studied. Results showed it to be necessary to eliminate interferences due to aluminum, iron and phosphate ions in amounts normally present in soils. Enough EDTA solution at pH 9.0 was added to the soil extract until a concentration of 0.01 M in EDTA was reached.

  14. Trueness investigation of routine creatinine assays on nine homogeneous systems in Beijing demonstrates an encouraging outcome that meets clinical requirements

    Institute of Scientific and Technical Information of China (English)

    LIU Yan; XU Guo-bin

    2010-01-01

    Background Serum creatinine (Scr) measurement plays a key role in glomerular filtration rate estimation (eGFR),chronic kidney disease (CKD) diagnosis as well as CKD treatment. However, the test results of Scr from different laboratories vary significantly. In order to get comparable results, the European in vitro diagnostic (IVD) directive requires traceability to reference methods and materials. The purpose of this study was to verify the effect of traceability implementation by investigating the trueness of creatinine measurement on nine homogenous systems in Beijing. Methods Commutable frozen human serum reference material, National Institute of Standards & Technology (NIST) Standard Reference Material (SRM) 967, was used to verify the trueness of Scr measurement results from nine homogeneous analytical systems of seven companies which are the most widely used systems in Beijing's third-grade hospitals. The methods referred to the Jaffe's and Enzymatic methods. Results from nine routine measurement systems were assessed using two criteria: biological variability and Clinical Laboratory Improvement Amendments' 88 (CLIA' 88).We simulated a series of broken lines representing the limits of SD and bias that would produce a relative increase (or decrease) of 10% and 20% in the measurement error when estimating GFR (MEeGFR) using the isotope dilution mass spectrometry (IDMS)-traceable Modification of Diet in Renal Disease (MDRD) Study equation. Results of the College of American Pathologists (CAP) 2008-B LN24 Survey were compared with our investigation results. Results Compared with the total error criteria of biological variability, Ortho (traceable to IDMS) met the minimum acceptable criteria; Roche (Jaffe), Roche (Enzymatic), Shino and Daiichi met the desirable criteria at level Ⅰ. At level Ⅱ, Ortho (traceable to gas chromatography/isotope dilution mass spectrometry, GC/IDMS), Dade Behring and Beckman (traceable to rate Jaffe) met the minimum acceptable

  15. Coupling gravitational and flow field-flow fractionation, and size-distribution analysis of whole yeast cells.

    Science.gov (United States)

    Sanz, Ramsés; Puignou, Lluís; Galceran, Maria Teresa; Reschiglian, Pierluigi; Zattoni, Andrea; Melucci, Dora

    2004-08-01

    This work continues the project on field-flow fractionation characterisation of whole wine-making yeast cells reported in previous papers. When yeast cells are fractionated by gravitational field-flow fractionation and cell sizing of the collected fractions is achieved by the electrosensing zone technique (Coulter counter), it is shown that yeast cell retention depends on differences between physical indexes of yeast cells other than size. Scanning electron microscopy on collected fractions actually shows co-elution of yeast cells of different size and shape. Otherwise, the observed agreement between the particle size distribution analysis obtained by means of the Coulter counter and by flow field-flow fractionation, which employs a second mobile phase flow as applied field instead of Earth's gravity, indicates that yeast cell density can play a major role in the gravitational field-flow fractionation retention mechanism of yeast cells, in which flow field-flow fractionation retention is independent of particle density. Flow field-flow fractionation is then coupled off-line to gravitational field-flow fractionation for more accurate characterisation of the doubly-fractionated cells. Coupling gravitational and flow field-flow fractionation eventually furnishes more information on the multipolydispersity indexes of yeast cells, in particular on their shape and density polydispersity.

  16. Concomitant spuriously elevated white blood cell count, a previously underestimated phenomenon in EDTA-dependent pseudothrombocytopenia.

    Science.gov (United States)

    Xiao, Yufei; Xu, Yang

    2015-01-01

    The proportion and potential risk of concomitant spuriously elevated white blood cell count (SEWC) are underestimated in ethylenediaminetetraacetic acid (EDTA)-dependent pseudothrombocytopenia (PTCP). The proportion, kinetics and prevention of SEWC remain poorly understood. A total of 25 patients with EDTA-dependent PTCP were enrolled in this study. With the hematology analyzer Coulter LH 750, we determined the time courses of WBC count, WBC differential and platelet count in EDTA- and sodium citrate-anticoagulated blood, respectively. Blood smears were prepared to inspect the presence of platelet clumps using light microscopy. The effect of automatic instrumental correction on the extent of SEWC was evaluated. The proportion of SEWC was 92% in EDTA-dependent PTCP and 73.9% of SEWCs were within the normal range. The development of SEWC was time-dependent, and neutrophils and lymphocytes were the main subpopulations involved in SEWC. A strong and significant correlation (r = 0.9937, p EDTA were significantly higher than their basal counts, respectively, p 0.05. A high proportion of concomitant SEWCs, which are mainly within normal range, are present in patients with EDTA-dependent PTCP. Proper interpretation of SEWC is crucial to avoid clinic errors. SEWC develops in a time-dependent pattern, although the Coulter LH 750 only partly mitigates the extent of SEWC, sodium citrate is able to effectively prevent SEWC.

  17. 幂函数型完全非线性函数原像分布的特征%Property of preimage distribution of perfect nonlinear function with the form of power functions

    Institute of Scientific and Technical Information of China (English)

    海昕; 戴清平; 李超

    2012-01-01

    完全非线性函数是特征为奇数的有限域上抗差分密码攻击最优的函数,目前已有的六类完全非线性函数都是2-1的.当Ⅱ(x)为Fqm上的Dembowski-Ostrom函数或者Coulter-Matthews函数时,从Fpm到Fq的完全非线性函数tr(aⅡ(x))的原像分布恰有两种取值,其中一种取值对应Fqm所有平方剩余元,另一种取值对应Fqm所有非平方剩余元.该结论在文中得到了证明.%Perfect nonlinear function is the optimal function on finite fields with odd character that can resist differential cryptanalysis. All the six classes of the already known perfect functions currently are 2-1. This study proved that when is Dembowski-Ostrom function or Coulter-Matthews function on , the preimage distribution of the perfect nonlinear function has just two kinds of values, one corresponds to all the elements of quadratic residual on , and the other kind corresponds to all the non quadratic residual on .

  18. Myxomycetes do Parque Zoobotânico de Teresina, Piauí, Brasil Myxomycetes of the Parque Zoobotânico - Teresina, Piauí, Brazil

    Directory of Open Access Journals (Sweden)

    Márcia Percília Moura Parente Ponte

    2003-03-01

    Full Text Available Visando ampliar o conhecimento sobre a mixobiota do Piauí, foram realizadas coletas no Parque Zoobotânico de Teresina (05º 05' 12'' S, 42º 48' 48'' WG. A vegetação do Parque caracteriza-se como um cerradão com representantes da mata de cocais, que oferecem substratos potencialmente favoráveis ao desenvolvimento de Myxomycetes. Esporocarpos foram coletados no período chuvoso e início da estiagem (março a junho de 1999, identificados e depositados no herbário UFP (Universidade Federal de Pernambuco. Foram obtidas 101 amostras, correspondentes a 19 espécies, distribuídas nas famílias Ceratiomyxaceae (Ceratiomyxa fruticulosa (Mull. Macbr., Clastodermataceae (Clastoderma debaryanum Blytt, Cribrariaceae (Cribraria microcarpa (Schrad. Pers., C. violacea Rex, Dictydium canceilatum* (Batsch. Macbr., Enteridiaceae (Lycogala exiguum* Morgan., Physaraceae (Craterium aureum* (Schum. Rost., Physarum bogoriense* Racib., P. melleum (Berk. & Br. Massee, P. nucleatum Rex, P. pusilium* (Berk. & Curt. G. Lister, Trichiaceae (Arcyria denudata (L. Wett., A. cinerea (Bull. Pers., Hemitrichia calyculata (Speg. Farr, H. serpula (Scop. Rost., Metatrichia vesparium (Batsch Nann.-Brem., Perichaena depressa Lib. e Stemonitaceae (Comatricha typhoides (Bull. Rost., Stemonitis smithii Macbr.. Exceto H. calyculata e H. serpula, todos os táxons são referidos pela primeira vez para o município de Teresina; as espécies assinaladas com um asterisco constituem novos registros para o Piauí.Aiming to increase the knowledge about the myxobiota of Piauí State, Brazil, collections were done in the undertaken Zoobotanical Park of Teresina (05° 05' 12'' S, 42° 48' 48'' WG. The vegetation of the Park is a savanna ("cerradão" with "cocais" forest representative, offering substrates potentially favorable to the development of Myxomycetes. Sporocarps were collected in the rainy season (March to June 1999, identified and deposited at the UFP herbarium (Federal University

  19. Impacts of forest harvesting on mobilization of Hg and MeHg in drained peatland forests on black schist or felsic bedrock.

    Science.gov (United States)

    Ukonmaanaho, Liisa; Starr, Mike; Kantola, Marjatta; Laurén, Ari; Piispanen, Juha; Pietilä, Heidi; Perämäki, Paavo; Merilä, Päivi; Fritze, Hannu; Tuomivirta, Tero; Heikkinen, Juha; Mäkinen, Jari; Nieminen, Tiina M

    2016-04-01

    Forest harvesting, especially when intensified harvesting method as whole-tree harvesting with stump lifting (WTHs) are used, may increase mercury (Hg) and methylmercury (MeHg) leaching to recipient water courses. The effect can be enhanced if the underlying bedrock and overburden soil contain Hg. The impact of stem-only harvesting (SOH) and WTHs on the concentrations of Hg and MeHg as well as several other variables in the ditch water was studied using a paired catchment approach in eight drained peatland-dominated catchments in Finland (2008-2012). Four of the catchments were on felsic bedrock and four on black schist bedrock containing heavy metals. Although both Hg and MeHg concentrations increased after harvesting in all treated sites according to the randomized intervention analyses (RIAs), there was only a weak indication of a harvest-induced mobilization of Hg and MeHg into the ditches. Furthermore, no clear differences between WTHs and SOH were found, although MeHg showed a nearly significant difference (p = 0.06) between the harvesting regimes. However, there was a clear bedrock effect, since the MeHg concentrations in the ditch water were higher at catchments on black schist than at those on felsic bedrock. The pH, suspended solid matter (SSM), dissolved organic carbon (DOC), and iron (Fe) concentrations increased after harvest while the sulfate (SO4-S) concentration decreased. The highest abundances of sulfate-reducing bacteria (SRB) were found on the sites with high MeHg concentrations. The biggest changes in ditch water concentrations occurred first 2 years after harvesting.

  20. Fifteen-year patterns of soil carbon and nitrogen following biomass harvesting

    Science.gov (United States)

    Kurth, Valerie J.; D'Amato, Anthony W.; Palik, Brian J.; Bradford, John B.

    2014-01-01

    The substitution of forest-derived woody biofuels for fossil fuel energy has garnered increasing attention in recent years, but information regarding the mid- and long-term effects on soil productivity is limited. We investigated 15-yr temporal trends in forest floor and mineral soil (0–30 cm) C and N pools in response to organic matter removal treatments (OMR; stem-only harvest, SOH; whole-tree harvest, WTH; and whole-tree plus forest floor removal, FFR) at three edaphically distinct aspen (Populus tremuloides Michx. and P. grandidentata Michx.) forests in the Great Lakes region. The OMR and temporal effects were generally site specific, and both were most evident in the forest floor and combined profile (mineral soil and forest floor) compared with the mineral soil alone. Forest floor and combined profile C and N pools were generally similar in the SOH and WTH treatments, suggesting that slash retention has little impact on soil C and N in this time frame. Temporal changes in C and N at one of the three sites were consistent with patterns documented following exotic earthworm invasion, but mineral soil pools at the other two sites were stable over time. Power analyses demonstrated that significant effects were more likely to be detected for temporal differences than the effects of OMR and in the combined profile than in the mineral soil. Our findings are consistent with previous work demonstrating that OMR effects on soil C and N pools are site specific and more apparent in the forest floor than the mineral soil.

  1. Screening and identification of the antagonistic organisms against Colletotrichum coccodes on potato%马铃薯炭疽病(Colletotrichum coccodes)拮抗菌株的筛选及鉴定

    Institute of Scientific and Technical Information of China (English)

    王涵琦; 畅涛; 杨成德; 陈秀蓉

    2014-01-01

    采用对峙培养法对马铃薯炭疽病的拮抗菌株进行了筛选和鉴定.结果表明,拮抗菌株ZHA10对炭疽病病原菌具有良好的抑制作用,抑制率达76.43%,其可以使病原菌菌丝变形和裂解;ZHA10菌体短杆状、革兰氏阳性、大小为(0.5~0.75)μm×(1.5~3.1)μm、芽胞中至尖端生.通过生理生化测定、16S rDNA以及gyrB序列分析,鉴定ZHA10为枯草芽胞杆菌(Bacillus subtilis).此外,ZHA10还对番茄早疫病菌(Alternaria solani)、马铃薯干腐病菌(Fusarium oxysporum)、孜然根腐病菌(Fusarium solani)、小麦根腐病菌(Bipolaris sorokiniana)、黄瓜枯萎病菌(Fusarium oxysporum)和马铃薯褐腐病菌(Stysanus stemonitis)均具有较强的拮抗作用.ZHA10抑菌能力良好,抑菌谱宽,具有进一步开发应用的潜力.

  2. Initial soil respiration response to biomass harvesting and green-tree retention in aspen-dominated forests of the Great Lakes region

    Science.gov (United States)

    Kurth, Valerie J.; Bradford, John B.; Slesak, Robert A.; D'Amato, Anthony W.

    2014-01-01

    Contemporary forest management practices are increasingly designed to optimize novel objectives, such as maximizing biomass feedstocks and/or maintaining ecological legacies, but many uncertainties exist regarding how these practices influence forest carbon (C) cycling. We examined the responses of soil respiration (Rs) to biomass harvesting and green-tree retention in an effort to empirically assess their impacts on C cycling. We measured Rs and soil microclimatic variables over four growing seasons following implementation of these management practices using a fully replicated, operational-scale experiment in aspen-dominated forests in northern Minnesota. Treatments included three levels of biomass removal within harvested areas: whole-tree harvest (no slash deliberately retained), 20% slash retained, and stem-only harvest (all slash retained), and two levels of green-tree retention: 0.1 ha aggregate or none. The relative amount of biomass removed had a negligible effect on Rs in harvested areas, but treatment effects were probably obscured by heterogeneous slash configurations and rapid post-harvest regeneration of aspen in all of the treatments. Discrete measurements of Rs and soil temperature within green-tree aggregates were not discernible from surrounding harvested areas or unharvested control stands until the fourth year following harvest, when Rs was higher in unharvested controls than in aggregates and harvested stands. Growing season estimates of Rs showed that unharvested control stands had higher Rs than both harvested stands and aggregates in the first and third years following harvest. Our results suggest that retention of larger forest aggregates may be necessary to maintain ecosystem-level responses similar to those in unharvested stands. Moreover, they highlight the innate complexity of operational-scale research and suggest that the initial impacts of biomass harvest on Rs may be indiscernible from traditional harvest in systems where incidental

  3. Phylogeny and taxonomic revision of Microascaceae with emphasis on synnematous fungi.

    Science.gov (United States)

    Sandoval-Denis, M; Guarro, J; Cano-Lira, J F; Sutton, D A; Wiederhold, N P; de Hoog, G S; Abbott, S P; Decock, C; Sigler, L; Gené, J

    2016-01-01

    The taxonomy of the synnematous genera Cephalotrichum, Doratomyces and Trichurus, and other related genera Gamsia, Wardomyces and Wardomycopsis, has been controversial and relies mainly on morphological criteria. These are microascaceous saprobic fungi mostly found in air and soil and with a worldwide distribution. In order to clarify their taxonomy and to delineate generic boundaries within the Microascaceae, we studied 57 isolates that include clinical, environmental and all the available ex-type strains of a large set of species by means of morphological, physiological and molecular phylogenetic analyses using DNA sequence data of four loci (the ITS region, and fragments of rDNA LSU, translation elongation factor 1α and β-tubulin). The results demonstrate that Cephalotrichum, Doratomyces and Trichurus are congeneric and the genus Cephalotrichum is accepted here with Echinobotryum as a further synonym. The genera Acaulium and Fairmania, typified by A. albonigrescens and F. singularis, respectively, are distinct from Microascus and Scopulariopsis, Gamsia is distinct from Wardomyces, and Wardomycopsis is confirmed as a separate genus in the Microascaceae. Two new species of Cephalotrichum are described as C. brevistipitatum and C. hinnuleum. Nine new combinations are proposed, i.e. Acaulium acremonium, A. caviariforme, Cephalotrichum asperulum, C. columnare, C. cylindricum, C. dendrocephalum, C. gorgonifer, Gamsia columbina and Wardomyces giganteus. A neotype is designed for C. stemonitis. Lectotypes and epitypes are designated for A. acremonium, A. albonigrescens, C. gorgonifer, C. nanum and W. anomalus. Cephalotrichum cylindricum, C. microsporum, F. singularis and Gamsia columbina are also epitypified with new specimens. Descriptions of the phenotypic features and dichotomous keys for identification are provided for accepted species in the different genera.

  4. Carbon and nitrogen pools and mineralization rates in boreal forest soil after stump harvesting

    Science.gov (United States)

    Kaarakka, Lilli; Hyvönen, Riitta; Strömgren, Monika; Palviainen, Marjo; Persson, Tryggve; Olsson, Bengt A.; Helmisaari, Heljä-Sisko

    2016-04-01

    The use of forest-derived biomass has steadily increased in the Finland and Sweden during the past decades. Thus, more intensive forest management practices are becoming more common in the region, such as whole-tree harvesting, both above- and belowground. Stump harvesting causes a direct removal of carbon (C) in the form of biomass from the stand and can cause extensive soil disturbance, which in turn can result in increased C mineralization. In this study, the effects of stump harvesting on soil C and nitrogen (N) mineralization, and soil surface disturbance were studied at two different clear-felled Norway spruce (Picea abies) stands in Central Finland. The treatments were conventional stem-only harvesting combined with mounding (WTH) and stump harvesting (i.e. complete tree harvesting) combined with mounding (WTH+S). Logging residues were removed from all study sites. Soil samples down to a depth of 20 cm were systematically collected from the different soil disturbance surfaces (undisturbed soil, the mounds and the pits) 12-13 years after final harvest. Soil samples were incubated in the laboratory to determine the C and N mineralization rates. In addition, total C and N pools were estimated for each disturbance class and soil layer. Soil C and N pools were lower following stump harvesting, however, no statistically significant treatment effect was detected. Instead, C mineralization responses to treatment intensity was site-specific. C/N-ratio and organic matter content were significantly affected by harvest intensity. The observed changes in C and N pools appear to be related to the intrinsic variation of the surface disturbance and soil characteristics, and harvesting per se, rather than treatment intensity. Long-term studies are however needed to draw long-term conclusions whether stump harvesting significantly changes soil C and nutrient dynamics.

  5. Dielectric breakdown of cell membranes.

    Science.gov (United States)

    Zimmermann, U; Pilwat, G; Riemann, F

    1974-11-01

    With human and bovine red blood cells and Escherichia coli B, dielectric breakdown of cell membranes could be demonstrated using a Coulter Counter (AEG-Telefunken, Ulm, West Germany) with a hydrodynamic focusing orifice. In making measurements of the size distributions of red blood cells and bacteria versus increasing electric field strength and plotting the pulse heights versus the electric field strength, a sharp bend in the otherwise linear curve is observed due to the dielectric breakdown of the membranes. Solution of Laplace's equation for the electric field generated yields a value of about 1.6 V for the membrane potential at which dielectric breakdown occurs with modal volumes of red blood cells and bacteria. The same value is also calculated for red blood cells by applying the capacitor spring model of Crowley (1973. Biophys. J. 13:711). The corresponding electric field strength generated in the membrane at breakdown is of the order of 4 . 10(6) V/cm and, therefore, comparable with the breakdown voltages for bilayers of most oils. The critical detector voltage for breakdown depends on the volume of the cells. The volume-dependence predicted by Laplace theory with the assumption that the potential generated across the membrane is independent of volume, could be verified experimentally. Due to dielectric breakdown the red blood cells lose hemoglobin completely. This phenomenon was used to study dielectric breakdown of red blood cells in a homogeneous electric field between two flat platinum electrodes. The electric field was applied by discharging a high voltage storage capacitor via a spark gap. The calculated value of the membrane potential generated to produce dielectric breakdown in the homogeneous field is of the same order as found by means of the Coulter Counter. This indicates that mechanical rupture of the red blood cells by the hydrodynamic forces in the orifice of the Coulter Counter could also be excluded as a hemolysing mechanism. The detector

  6. Is the Beckmann rearrangement a concerted or stepwise reaction? A computational study.

    Science.gov (United States)

    Yamabe, Shinichi; Tsuchida, Noriko; Yamazaki, Shoko

    2005-12-23

    [reaction: see text] RB3LYP calculations were performed on the Beckman rearrangement by the use of three substrates, acetone oxime (1), acetophenone oxime (2), and cyclohexanone oxime (3). Acidic solvents were modeled by H+ (CH3COOH)3 and H3O+ (H2O)6, and reaction paths were determined precisely. For 1, a two-step process involving a sigma-type cationic complex was obtained. For 2, a three-step process with pi- and sigma-type complexes was found in H+ (CH3COOH)3 and a two-step process involving a sigma-type cationic complex was obtained in H3O+ (H2O)6. However, for 3, a concerted process without pi and sigma complexes was calculated, which leads to the product, epsilon-caprolactam. Three different mechanisms were explained in terms of FMO theory.

  7. Biocromos (pigmentos de invertebrados marinhos: I - Briozoarios

    Directory of Open Access Journals (Sweden)

    Gilberto G. Villela

    1948-06-01

    Full Text Available In the present paper the author reported the results obtained for the chemical caracterization of the pigments of some species of Bryozoa (Polyzoa, living in the bay and the coast near Rio de Janeiro. The pigments (biochromes of "Bugula neritina", "Schizoporella unicornis", "Steganoporella magnilabris", "Bugula flabellata" and "Trigonospora sp", were extracted and the results showed that carotene was found in all the species, except, "Bugula neritina" and "Bugula flabellata". A new water-soluble pigment was described for "Bugula neritina". Spectrophotometric curves obtained with the Beckman spectrophotometer are reported for the "Bugula" pigment and for the carotenoids. Chromatographic analysis and the treatment with immiscible solvents were performed for all the extracts of the animals considered. A study of the biochromes of other "phyla" will be published in a near future.

  8. STIMULATIONG EFFECT OF LOW DOSE 147Pm ON DNA REPAIR IN SPERMIOGENIC STAGES

    Institute of Scientific and Technical Information of China (English)

    朱寿彭; 王六一

    1995-01-01

    For labelling spermatid DNA,male BALB/c mice are injected intratesticularyly with 3H-TdR in advance.After 36d of labelling,spermatozoa are sampled which is just the time required for the germ cells to mature.The period from intraperitoneal injection of 147Pm to sampling spermatozoa is 12d.After sperm cells have been lysed.30ml eluent is added by wriggle pump.Finally,both the DNA remained on the filters and filtrated bottles are determined with a Beckman liquid scintillation device.Results show that after small dose internal irradiation with 185Bq/g of 147Pm,an increase in amount of sperm DNA on the filter observed is considerably higher than the control group.This indicates that low level internal radiation of 147Pm has tendency to stimulate the DNA repair in spermiogenic stages.

  9. A Mössbauer and X-ray powder diffraction study of some ferrous hematinics.

    Science.gov (United States)

    Coe, E M; Bowen, L H; Bereman, R D

    1995-06-01

    Iron deficiency anemia is a relatively common illness that can arise from a number of different causes. Three ferrous salts are usually used in its treatment: ferrous fumarate, gluconate, and sulfate. They are administered orally and are relatively well tolerated. These hematinics have been studied by Mössbauer spectroscopy and X-ray powder diffraction, and can easily be distinguished by both techniques. It was found that the two ferrous sulfates studied (Eckerd and SmithKline Beckman Co.) most closely resemble the monohydrate by comparison of the X-ray powder pattern with those of the JCPDS. Both the ferrous fumarate (Femiron) and gluconate (Spring Valley) had approximately 10% ferric iron present. To the authors' knowledge, this is the first reported Mössbauer spectrum for ferrous fumarate.

  10. The establishment of thyroid hormone reference intervals of pregnant women in Liaocheng city%聊城市妊娠妇女甲状腺激素参考区间的建立

    Institute of Scientific and Technical Information of China (English)

    白书堂; 李姗姗; 王素红

    2014-01-01

    Objective:To establish reference intervals of thyroid hormone in pregnant women of our laboratory.The thyroid hormone include thyroid stimulating hormone (TSH), free tetraiodothyronine (FT4) and free triiodothyronine (FT3).Methods:They were divided into three groups with total 826 pregnant women, based on different stages of pregnancy, such as the first, second or late. Thyroid hormone was tested by chemiluminescence in Beckman access2 chemiluminescence analyzer and supporting reagent. Then, we could try to build reference intervals of thyroid hormone in pregnant women.Results:The reference intervals of TSH was as below, 0.11~2.88 uIU/ml in first trimester of pregnancy, 0.24~3.31 uIU/ml in second trimester of pregnancy, and 0.55~3.78 uIU/ml in late trimester of pregnancy. The range of FT4 was as follows, 0.69~1.13 ng/dl in first trimester of pregnancy, 0.56~0.99 ng/dl in second trimester of pregnancy, and 0.49~0.89 ng/dl in late trimester of pregnancy. The reference range of FT3 was 2.67~4.03 pg/ml, 2.35~3.56 pg/ml, and 2.08~3.25 pg/ml respectively, in different trimester of pregnancy.Conclusions:We made reference range of thyroid hormone in pregnant women, and the three mainly thyroid hormone was TSH、FT4、FT3. The range was difference with instructions of Beckman access2 chemiluminescence analyzer. Finally, we suggested that we should establish reference range of thyroid hormone in all laboratory based on different instruments and the crowd.%目的:建立本实验室妊娠妇女促甲状腺激素(TSH)、游离甲状腺素(FT4)和游离三碘甲状腺原氨酸(FT3)的参考区间。方法:将826例妊娠妇女分为妊娠早中晚期3组,用Beckman access2化学发光分析仪及其配套试剂检测TSH、FT4、FT3,初步建立参考区间。结果:TSH参考区间:妊娠早期0.11~2.88 uIU/ml,妊娠中期0.24~3.31 uIU/ml,妊娠晚期0.55~3.78 uIU/ml。FT4参考区间:妊娠早期0.69~1.13 ng/dl,妊娠中期0.56~0.99 ng

  11. Effects of water on reactions for waste treatment, organic synthesis, and bio-refinery in sub- and supercritical water.

    Science.gov (United States)

    Akizuki, Makoto; Fujii, Tatsuya; Hayashi, Rumiko; Oshima, Yoshito

    2014-01-01

    Current research analyzing the effects of water in the field of homogeneous and heterogeneous reactions of organics in sub- and supercritical water are reviewed in this article. Since the physical properties of water (e.g., density, ion product and dielectric constants) can affect the reaction rates and mechanisms of various reactions, understanding the effects that water can have is important in controlling reactions. For homogeneous reactions, the effects of water on oxidation, hydrolysis, aldol condensation, Beckman rearrangement and biomass refining were introduced including recent experimental results up to 100 MPa using special pressure-resistance equipment. For heterogeneous reactions, the effects of ion product on acid/base-catalyzed reactions, such as hydrothermal conversion of biomass-related compounds, organic synthesis in the context of bio-refinery, and hydration of olefins were described and how the reaction paths are controlled by the concentration of water and hydrogen ions was summarized.

  12. Discussing epigenetics in Southern California: a report from the International Symposium on Epigenetic Control and Cellular Plasticity, UCI, December 15-16, 2011.

    Science.gov (United States)

    Rattner, Barbara P

    2012-04-01

    With the goal of discussing how epigenetic control and chromatin remodeling contribute to the various processes that lead to cellular plasticity and disease, this symposium marks the collaboration between the Institut National de la Santé et de la Recherche Médicale (INSERM) in France and the University of California, Irvine (UCI). Organized by Paolo Sassone-Corsi (UCI) and held at the Beckman Center of the National Academy of Sciences at the UCI campus December 15-16, 2011, this was the first of a series of international conferences on epigenetics dedicated to the scientific community in Southern California. The meeting also served as the official kick off for the newly formed Center for Epigenetics and Metabolism at the School of Medicine, UCI (http://cem.igb.uci.edu).

  13. Enzymatic assay of total cholesterol in serum or plasma by amperometric measurement of rate of oxygen depletion following saponification.

    Science.gov (United States)

    Kumar, A; Christian, G D

    1977-01-17

    A method for serum or plasma cholesterol assay involving amperometric measurement of the rate of oxygen depletion in the cholesterol oxidase-catalyzed oxidation of cholesterol is described. The hydrolysis of the serum cholesterol esters is accomplished by saponification of 50 mul of sample with 0.2 ml of ethanolic KOH (1.0 mol/1) containing 0.5% Triton X-100 for 5 min at 75 degrees C. The rate of oxygen consumption in a 25-mul aliquot of this is measured with a Clark electrode in a Beckman Glucose Analyzer and the assay takes about one minute after incubation; results are read digitally on the instrument. The analyzer cell contains 1 ml of 1 M phosphate buffer, pH 7.4, with 100 mg sodium cholate/100 ml and 0.1-0.2 U cholesterol oxidase.

  14. ON THE STATUS OF CODACOND IN PHONOLOGY

    Directory of Open Access Journals (Sweden)

    Jill N. Beckman

    2004-12-01

    Full Text Available Onset/coda feature licensing asyrnmetries have been extensively studied in the generative phonological literature. Pre-Optimality Theory analyses of such asymmetries rely on positional licensing or positional rnarkedness statements, ofwhich Ito's (1986,1989 Coda Conditionis the best-known. In OT, a different approach to onsetlcoda asyrnmetries has emerged: onset-specific faithfulness (Padgett 1995; Beckman 1999; Lombardi 1999,2001. In a recent paper, Lombardi (2001 argues that both onset faithfulness and the Coda Condition are required to account for the range of repairs associated with coda/onset asymrnetries in the licensing of place features, arguing that positional faithfulness alone cannot generate epenthesis as a repair strategy. In this paper, I show, through analyses of Tamil and Axininca Carnpa, that the Coda Condition is not required to generate place-driven epenthesis; rather, epenthesis emerges from the interaction of onset faithfulness with other, independently motivated faithfulness and syllable well-formedness constraints.

  15. Exploring variation in accuracy and contrast for sibilant fricatives at the onset of fricative acquisition

    Science.gov (United States)

    Nicholson, Hannele Buffy Marie

    Children's speech differs from adult speech in the many ways, including in its phonetic characteristics. A central question for researchers interested in child speech sound acquisition is when and how a child acquires robust adult-like contrasts. In this thesis, I present a protocol for the analysis of the English sibilant fricatives "s" and "sh". Sibilant fricatives are of interest because they are late-acquired sounds that require articulatory-aerodynamic coordination, and are contrastively necessary in multiple languages around the world, English especially. Given the turbulent nature of the sound spectrum of fricative consonants, few agreed upon measures exist. Holliday, Reidy, Beckman and Edwards (In Preparation) propose that peak equivalent rectangular bandwidth is a psychoacoustically appropriate measure for modeling the robustness of phonological contrast between sibilant fricative types. The robustness measures put forth by Holliday et al. are applied to data from the speech of toddlers aged 28-39 months and are discussed.

  16. Applicability of a prototype for determination of absorbed dose using brachytherapy equipment with Ir-192 sources; Aplicabilidade de um prototipo para determinacao da dose absorvida utilizando equipamentos de braquiterapia com fontes de IR-192

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Vivianne Lucia Bormann; Almeida, Mayara Gabriella Oliveira de; Vieira, Rafaela Etelvina de Amorim; Silva, Waldecy Ananias da; Nascimento, Rizia Keila, E-mail: vlsouza@cnen.gov.br, E-mail: mayaradqf@hotmail.com, E-mail: rodriguesss@hootmail.com, E-mail: waldecy@cnen.gov.br, E-mail: riziakeila@hotmail.com [Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil)

    2014-07-01

    This work aims at the development and improvement of a device to perform the absolute dosimetry sources of Ir-192 using the Fricke solution contained in a flask. The Fricke solution used was prepared using amounts of ferrous ammonium sulfate, sodium chloride and sulfuric acid, diluted with water tri distilled pre-established in the literature. The spectrophotometer used was a UV-VIS spectrophotometer (Beckman DU-640 Counter) for measuring the optical density at wavelength 304 nm. The calculation for determining the radial dose takes into account the radial distance and the angle formed with the transverse axis of the source. As the results obtained can be seen that the states of Pernambuco, Ceara, Paraiba e Piaui are in accordance with the recommendations of international standards of the International Atomic Energy Agency (IAEA), which considers not acceptable a difference greater than 5% of prescribed dose and measured dose.

  17. Determination of free and total carnitine with a random-access chemistry analyzer.

    Science.gov (United States)

    Wan, L; Hubbard, R W

    1998-04-01

    Carnitine deficiency presents as a major problem in fatty acid oxidation. The use of a plasma carnitine assay can rapidly help to describe this deficiency. The method we describe here requires two simple steps of sample preparation, followed by automated analysis with the Beckman Synchron CX4 random-access chemistry analyzer. The goal of this method development was to reduce the cost of analysis and to allow a greater number of laboratories to perform this assay on demand within 1 h for both free and total carnitine. The method has a linearity of 0-150 micromol/L and a detection limit of 5 micromol/L. The inter- and intraday CVs are <20%. The method agreed closely with both the widely used RIA and spectrophotometric methods.

  18. Reproducing sterile neutrinos and the behavior of flavor oscillations with superconducting-magnetic proximity effects

    Science.gov (United States)

    Baker, Thomas E.

    2016-03-01

    The physics of a superconductor subjected to a magnetic field is known to be equivalent to neutrino oscillations. Examining the properties of singlet-triplet oscillations in the magnetic field, a sterile neutrino is suggested to be represented by singlet Cooper pairs and moderates flavor oscillations between three flavor neutrinos (triplet Cooper pairs). A superconductor-exchange spring system's rotating magnetization profile is used to simulate the mass-flavor oscillations in the neutrino case and the physics of neutrino oscillations are discussed. Connecting the condensed matter system and the particle physics system with this analogy may allow for the properties of the condensed matter system to inform neutrino experiments. Support is graciously acknowledged from the Pat Beckman Memorial Scholarship from the Orange County Chapter of the Achievement Rewards for College Scientists Foundation.

  19. Interlaboratory comparison of enzymatic methods for serum glucose determination.

    Science.gov (United States)

    Gochman, N; Ryan, W T; Sterling, R E; Widdowson, G M

    1975-03-01

    This report describes an interlaboratory comparison of enzymatic serum glucose methods as currently applied in several instrumental adaptations. Four spectrophotometric, hexokinase-based methods used with the Du Pont "aca," the Abbott "Bichromatic Analyzer," the Aminco "Rotochem," and the Technicon "AutoAnalyzer ll" were compared with glucose oxidase-based methods as used with the Beckman "Glucose Analyzer" and the "AutoAnalyzer ll." We analyzed both normal samples and samples that contained potential interfering substances or were otherwise abnormal. Although all methods were satisfactorily precise, methodological bias was noted in several cases, particularly with abnormal specimens. The hexokinase-based methods were generally more variable. The most consistent data were obtained from the two glucose oxidase methods and the Du Pont aca. Results of this study comprise part of the background information required for development of an accurate glucose reference method under the auspices of the AACC Standards Committee.

  20. A High-affinity Activator of G551D-CFTR Chloride Channel Identified By High Throughput Screening

    Institute of Scientific and Technical Information of China (English)

    ZHAO Lu; HE Cheng-yan; LIU Yan-li; ZHOU Hong-lan; ZHOU Jin-song; SHANG De-jing; YANG Hong

    2004-01-01

    A stably transfected CHO cell line coexpressing G551D-CFTR and iodide-sensitive yellow fluorescent protein mutant EYFP-H148Q-I152L was successfully established and used as assay model to identify small-molecule activators of G551D-CFTR chloride channel from 100000 diverse combinatorial compounds by high throughput screening on a customized Beckman robotic system. A bicyclooctane compound was identified to activate G551D-CFTR chloride channel with high-affinity(Kd=1.8 μmol/L). The activity of the bicyclooctane compound is G551D-CFTR-specific, reversible and non-toxic. The G551D-CFTR activator may be useful as a tool to study the mutant G551D-CFTR chloride channel structure and transport properties and as a candidate drug to cure cystic fibrosis caused by G551D-CFTR mutation.

  1. Comparison of blood glucose test results between point-of-care testing glucose meter and biochemical analyzer%POCT血糖仪与生化分析仪血糖检测结果的比对试验及分析

    Institute of Scientific and Technical Information of China (English)

    刘加红

    2014-01-01

    Objective To explore precision and accuracy of point-of-care testing(POCT) blood glucose meter through comparing blood glucose results between POCT glucose meter and biochemical analyzer. Methods Selected 4 sets of POCT glucose meter to detect the same sample and compared blood glucose results between Beckman CX-9 biochemical analyzer and POCT blood glucose meter. Results Measured results of Beckman CX-9 biochemical analyzer and POCT glucose meter were highly correlated(P>0.05), differences were not statistically significant. Conclusion POCT blood glucose meter is a consistent and convenient detection method for measuring blood glucose results as biochemical analyzer, so it is worthy of clinical promotion and application.%目的对比床旁检测(POCT)血糖仪与生化分析仪对血糖测定的结果,对POCT血糖仪的精密度与准确度进行探讨。方法选用4台POCT血糖仪对同一标本进行测定,并比较贝克曼CX-9生化分析仪与POCT血糖仪的测定结果。结果贝克曼CX-9生化分析仪与POCT血糖仪的测定结果高度相关(P>0.05),差异不具有统计学意义。结论 POCT血糖仪与生化分析仪对血糖的测定结果具有一致性,且方便快捷,可作为急诊的检测方法,值得在临床过程中推广、应用。

  2. Research on the plasma biochemistry of cardiac function, liver function and renal function from healthy larval green turtles (Chelonia mydas) in captivity%绿海龟健康幼体心肝肾功能血液生化指标参考值研究

    Institute of Scientific and Technical Information of China (English)

    成奇; 潘连德

    2016-01-01

    采集了39只人工养殖的健康幼体绿海龟(1.5~2.5龄)、6只健康成体绿海龟(10~ 20龄)和3只野生成体绿海龟,用Beckman AU5800和TNI BECKMAN Access 2测定和分析了反映绿海龟心肝肾功能的17项血液生化指标,结果发现野生个体的生化指标与人工养殖的绿海龟差异较大,谷草转氨酶(P =0.002)、白蛋白(P =0.015)、碱性磷酸酶(P=O.010)、谷丙转氨酶(P=O.OIO)等均有明显差异,人工养殖绿海龟除碱性磷酸酶与年龄呈负相关外没有明显差异,不同性别的个体在生化指标上差异不显著.本实验结果与野生绿海龟各研究结果对比发现,野生个体的指标差异非常显著,难以作为健康指标的参考.本研究建立了人工绿海龟的心肝肾功能血液生化指标参考值,为野生绿海龟血液生化研究提供参考,为指导绿海龟养殖过程中的疾病早期诊断和预防提供依据.

  3. Crystalluria in marathon runners. 1. Standard marathon--males.

    Science.gov (United States)

    Irving, R A; Noakes, T D; Rodgers, A L; Swartz, L

    1986-01-01

    Epidemiological evidence suggests that marathon runners have a higher incidence of renal stone formation than occurs in the general population. Since crystalluria and stone disease are thought to be related, we subjected urine samples from a group of marathon runners to particle counting and sizing in a Coulter Counter equipped with a population accessory unit. The volume-size distribution curves so obtained were bimodal with one peak occurring in the 2-5 micron diameter range and a second in the 15-32 micron diameter range - a pattern that is remarkably similar to the distributions reported for recurrent idiopathic stone formers and distinctly different to those recorded for control subjects. Analyses by scanning electron microscopy and X-ray powder diffraction revealed other features which are regarded as typical of stone formers' crystalluria. These physicochemical data indicate that marathon runners may be at increased risk of urinary stone formation.

  4. Dynamics of polymer nanoparticles through a single artificial nanopore with a high-aspect-ratio.

    Science.gov (United States)

    Cabello-Aguilar, Simon; Chaaya, Adib Abou; Bechelany, Mikhael; Pochat-Bohatier, Céline; Balanzat, Emmanuel; Janot, Jean-Marc; Miele, Philippe; Balme, Sébastien

    2014-11-14

    The development of nanometric Coulter counters for nanoparticle detection is an attractive and promising field of research. In this work, we have studied the influence of the nanopore surface state on charged polymer nanoparticle translocations. To make this, the translocation of carboxylate modified polystyrene microspheres (diameter 40, 70 and 100 nm) has been investigated through two kinds of high aspect ratio nanopores (negative and uncharged). The latter were tailored by a single track-etched and atomic layer deposition technique. It was shown that the mobility and the energy barrier are strongly dependent on nanopore surface charge. Typically if the latter exhibits negative surface charge, the microsphere mobility increases and the global energy barrier of entrance inside the nanopore decreases with its diameter, converse to the uncharged nanopore.

  5. Involving patients with leg ulcers in developing innovations in treatment and management strategies.

    Science.gov (United States)

    McNichol, Elaine

    2014-09-01

    Securing greater patient and public involvement is a central theme of health policy in many countries ( Coulter, 2011 ) and is a growing health-care phenomenon ( Staniszewska et al, 2011 ; World Health Organization, 2011 ). The challenge is how to ensure these voices are heard among the plethora of 'professional' voices representing different stakeholders of provider organisations, higher education, health-care industries and governments. Historically, in the management of leg ulcers, the professional 'user' voice has had a stronger input than that of the patient. This article outlines an approach that addresses this by moving beyond the concept of 'involving' the patient to putting the patient voice first, so that it sets the direction and leads the process in identifying innovation priorities in the management and treatment of chronic leg ulcers.

  6. El liderazgo docente y su relación con el estilo gerencial de los pasantes de Comunicación Social de la Universidad del Zulia

    Directory of Open Access Journals (Sweden)

    Rosario Fonseca

    2007-09-01

    Full Text Available Se propuso determinar la relación entre el liderazgo docente y el estilo gerencial de los estudiantes de Comunicación Social, mención Periodismo Impreso de LUZ, de acuerdo a la clasificación de Robbins, Stephen y Coulter (1999, y Guédez (1996 y siguiendo una metodología descriptiva-correlacional. Se aplicó un cuestionario para docentes y estudiantes de los últimos semestres. Los resultados demuestran que existe relación entre el liderazgo de los docentes, en su mayoría de tipo Democrático-Situacional, y el estilo gerencial de los estudiantes, predominó el estilo Estratégico y Situacional.

  7. Inhibitory effect of substituted dextrans on MCF7 human breast cancer cell growth in vitro.

    Science.gov (United States)

    Morere, J F; Letourneur, D; Planchon, P; Avramoglou, T; Jozefonvicz, J; Israel, L; Crepin, M

    1992-12-01

    Substituted dextrans can reproduce some of the properties of heparin and can thus be used to alter cellular growth. We studied the effect of heparin (H108), dextran (D), carboxymethylbenzylamide dextran (CMDB) and carboxymethylbenzylamide sulfonate dextran (CMDBS) on the growth of human mammary cells of the MCF7 tumor line. The cells were cultured in minimum Eagle's medium containing 2% fetal calf serum without biopolymer, or with increasing concentrations of H108, D, CMDB or CMDBS. Growth curves were accurately based on cell counting using a Coulter counter. Cell distribution in the various phases of the cycle was analyzed by flow cytometry. Dose-dependent growth inhibitory effects (400-4000 micrograms/ml) were observed. The effect on MCF7 tumor cells was most apparent with CMDBS. The percentage of cells in the S phase decreased with preferential blocking in the G0/G1 phase. Pre-clinical studies can be anticipated as there is an absence of in vivo toxicity.

  8. Survival and activity of Streptococcus faecalis and Escherichia coli in tropical freshwater

    Energy Technology Data Exchange (ETDEWEB)

    Muniz, I.; Jimenez, L.; Toranzos, G.A.; Hazen, T.C. [Univ. of Puerto Rico, Rio Piedras (Puerto Rico)

    1988-12-31

    The survival of Streptococcus facecalis and Escherichia coli was studied in situ in a tropical rain forest watershed using membrane diffusion chambers. Densities were determined by acridine orange direct count and Coulter Counter. Population activity was determined by microautoradiography, cell respiration, and by nucleic acid composition. Densities of S. facecalis and E. coli decreased less than 1 log unit after 105 h as measured by direct count methods. Activity as measured by respiration, acridine orange activity, and microautoradiography indicated that both bacteria remained moderately active during the entire study. After 12 h, E. coli was more active than S. faecalis as measured by nucleic acid composition. E. coli and S. faecalis survived and remained active for more than 5 days. Consequently, both would seem to be unsuitable as indicators of recent fecal contamination in tropical waters.

  9. Interfacial electrokinetic characteristics before and after bioleaching microorganism adhesion to pyrite

    Institute of Scientific and Technical Information of China (English)

    LIU Jian-she; WANG Zhao-hui; CHEN Hong; ZHANG Yan-hua

    2006-01-01

    Zeta potentials of pyrite and Acidithiobacillusferrooxidans cultured by sulfur in different levels of ionic strength and pH values were measured by Coulter Delsa 440SX zeta potential determinator. Meanwhile, the effects of bacterial adhesion and bacterial concentration on zeta potential of pyrite after adsorption were investigated. The results show that with the increase of ionic strength,zeta potentials of pyrite decrease in the range of pH 2.5-10.5 and the isoelectric point(IEP) of mineral shifts to the left. It is also found that the specific adsorption on pyrite of chloride ion can affect zeta potentials of pyrite sharply. As bacterial adsorption occurs,IEP of pyrite shifts towards that of Acidithiobacillus ferrooxidans; as bacterial concentration is increscent, this tendency is even larger and more obvious. Finally, a reasonable explanation for above-mentioned experimental phenomena was given by electrical double layer model and surface ionization model.

  10. Glaciological and chemical studies on ice cores from Hans Tausen ice cap, Greenland

    DEFF Research Database (Denmark)

    Clausen, H.B.; Stampe, Mia; Hammer, C.U.

    2001-01-01

    to the thermo-nuclear tests in the atmosphere in the early 1960'ies. Many of the investigated parameters exhibit seasonal variations, e.g. d18O, acidity, Cl- and dust, therefore the study of the accumulation history of the ice cap improves our knowledge of the question if the mass balance of the Hans Tausen...... exist along the 1995 cores (MC95 and SC95) and finally detailed records of dust and water soluble ion concentrations exist on selected parts of MC95. To determine a time scale for the ice core is an important prerequisite for the interpretation of other records. The age scale is based on acid layers...... are selected for an analysis of dust and water soluble chemical components, including F-, CH3SO2-, Cl-, NO3-, SO42-, Na+, NH4+, K+, Mg2+ and Ca2+. Coulter counter technique was used for the dust measurements and the chemical analysis were carried out by ion chromatography....

  11. The crystal growth of barium flouride in aqueous solution

    Science.gov (United States)

    Barone, J. P.; Svrjcek, D.; Nancollas, G. H.

    1983-06-01

    The kinetics of growth of barium flouride seed crystals were investigated in aqueous solution at 25°C using a constant composition method, in which the supersaturation and ionic strength were maintained constant by the addition of titrants consisting of barium nitrate and potassium flouride solutions. The rates of reaction, studied over a range of supersaturation (σ ≈ 0.4 to 1.0), were interpreted in terms of crystal growth models. A spiral growth mechanism best describes the data, and scanning electron microscopy indicates a three-dimensional growth. In the presence of inorganic additives such as phosphate, however, induction periods precede a morphological two-dimensional crystallization. Coulter Counter results show little crystal agglomeration.

  12. Accurate platelet counting in an insidious case of pseudothrombocytopenia.

    Science.gov (United States)

    Lombarts, A J; Zijlstra, J J; Peters, R H; Thomasson, C G; Franck, P F

    1999-01-01

    Anticoagulant-induced aggregation of platelets leads to pseudothrombocytopenia. Blood cell counters generally trigger alarms to alert the user. We describe an insidious case of pseudothrombocytopenia, where the complete absence of Coulter counter alarms both in ethylenediaminetetraacetic acid blood and in citrate or acid citrate dextrose blood samples was compounded by the fact that the massive aggregates were exclusively found at the edges of the blood smear. Non-recognition of pseudothrombocytopenia can have serious diagnostic and therapeutic consequences. While the anti-aggregant mixture citrate-theophylline-adenosine-dipyridamole completely failed in preventing pseudothrombocytopenia, addition of iloprost to anticoagulants only partially prevented the aggregation. Only the prior addition of gentamicin to any anticoagulant used resulted in a complete prevention of pseudothrombocytopenia and enabled to count accurately the platelets.

  13. Reconnaissance surficial geologic map of the Taylor Mountains quadrangle, southwestern Alaska

    Science.gov (United States)

    Wilson, Frederic H.

    2015-09-28

    This map and accompanying digital files are the result of the interpretation of aerial photographs from the 1950s as well as more modern imagery. The area, long considered a part of Alaska that was largely not glaciated (see Karlstrom, 1964; Coulter and others, 1965; or Péwé, 1975), actually has a long history reflecting local and more distant glaciations. An unpublished photogeologic map of the Taylor Mountains quadrangle from the 1950s by J.N. Platt Jr. was useful in the construction of this map. Limited new field mapping in the area was conducted as part of a mapping project in the Dillingham quadrangle to the south (Wilson and others, 2003); however, extensive aerial photograph interpretation represents the bulk of the mapping effort. The accompanying digital files show the sources for each line and geologic unit shown on the map.

  14. Towards an All-Polymer Biosensor for Early Alzheimer's Disease

    DEFF Research Database (Denmark)

    Christiansen, Nikolaj Ormstrup; Heegaard, Niels

    for measuring this. Lab-on-a-chip biosensors are one of the fastest growing technologies for in vitro diagnostics. The combination of microfluidics and biosensing offers exciting possibilities for producing extremely sensitive and low cost applications for medicine and diagnostics. This thesis include studies...... of polymerized p-toluenesulfonate doped poly(3,4-ethylenedioxythiophene) (PEDOT:TsO) nanowires. The PEDOT:TsO nanowires are demonstrated to be promising temperature sensors. We also show that the nanowires can be gated with a backgating potential, which is a requirement for using it as a biosensor. The nanowires...... were also shown to be able to measure changes in larger biological samples as cells. This shows a promise for utilizing the PEDOT:TsO nanowires as Aβ42 biosensors. A prototype for a lab-on-a-chip system consisting of a double assay sorting channel and a electrochemical impedance spectroscopy coulter...

  15. [Study on targeting drug delivery system--the characteristics of methotrexate microsphere and experimental treatment of hepatic tumor in rats by arterial embolization].

    Science.gov (United States)

    Chen, Q H; Lu, W G; Ge, Q H; Sheng, Q; Zhang, Y; Xie, X H; Wang, Y; Wu, M C; Zhang, X H

    1991-01-01

    Preparation of methotrexate microsphere (MTX-ms) by emulsion-freezing technique was introduced and the experimental results proved that MTX entrapped in the microspheres exhibited good stabilities towards temperature, cobalt-60 radiation and light. The dissolution and inflation rate of the microspheres in pH 7.4 buffer solution at different times measured by Coulter counter was presented. Antitumor activity of MTX-ms after hepatic arterial embolization was examined in a model of liver tumor in Wistar rats. The group of rats treated with MTX-ms showed a rather significant reduction in tumor growth and more extended tumor necrosis as compared with the other groups, e.g. normal saline solution, MTX solution, placebo gelatin-ms and the results demonstrate that the effect of arterial chemoembolization used by MTX-ms is superior to that of the groups either using arterial chemotherapy or arterial embolization alone in treating rat liver cancer.

  16. Crop establishment of Silphium perfoliatum by precision seeding

    Directory of Open Access Journals (Sweden)

    Andreas Schäfer

    2015-12-01

    Full Text Available Silphium perfoliatum, a perennial composite from North America, has been found as a promising plant species during the search for alternative biogas substrates. Until now Silphium perfoliatum has been established by the time- and cost-intensive transplanting method. The precision sowing of Silphium perfoliatum reduces the process costs significantly. However, the bulky seed with mildness germination power complicates a well distributed and adequate plant establishment. In field tests a modified precision seeder was used for sowing Silphium perfoliatum which is primarily used for maize sowing.The hole diameter of the singling disc, the sowing coulter and the roller were modified. In the field tests the modified machinery proved an enhanced and uniform field emergence of Silphium perfoliatum.

  17. Grey scale enhancement of rabbit liver and kidney by intravenous injection of a new lipid-coated ultrasound contrast agent

    Institute of Scientific and Technical Information of China (English)

    Ping Liu; Yun-Hua Gao; Kai-Bin Tan; Zheng Liu; Song Zuo

    2004-01-01

    AIM: To assess the grey scale enhancement of a new lipidcoated ultrasound contrast agent in solid abdominal organs as liver and kidney.METHODS: Size distribution and concentration of the lipidcoated contrast microbubbles were analyzed by a Coulter counter. Two-dimensional (2D) second harmonic imaging of the hepatic parenchyma, the inferior vena cava and the right kidney of the rabbits were acquired before and after contrast agent injection. Images were further quantified by histogram in Adobe Photoshop 6.0. Time-intensity curves of hepatic parenchyma, inferior vena cava and renal cortex were generated from the original grey scale.RESULTS: The 2D images of hepatic parenchyma and cortex of the kidney were greatly enhanced after injection and the peak time could last more than 50 min.CONCLUSION: This new lipid ultrasound contrast agent could significantly enhance the grey scale imaging of the hepatic parenchyma and the renal cortex for more than 50 min.

  18. Improved performance of gravitational field-flow fractionation for screening wine-making yeast varieties.

    Science.gov (United States)

    Sanz, R; Torsello, B; Reschiglian, P; Puignou, L; Galceran, M T

    2002-08-09

    Performance of gravitational field-flow fractionation (GFFF) is improved here with respect to the ability to fractionate and distinguish different varieties of wine-making yeast from Saccharomyces cerevisiae. A new GFFF channel with non-polar walls has been employed to enhance fractionation selectivity and reproducibility. Since GFFF retention depends from first principles on particle size, Coulter counter measurements were performed in order to compare size distribution profiles with GFFF profiles. From such a comparison, GFFF was shown to be able to reveal differences in yeast cells other than size. This could make use of GFFF for screening different varieties of wine-making yeast towards future quality assessment procedures based on a possible correlation between yeast cell morphology indexes and quality indexes.

  19. Ultra-sensitive flow measurement in individual nanopores through pressure--driven particle translocation.

    Science.gov (United States)

    Gadaleta, Alessandro; Biance, Anne-Laure; Siria, Alessandro; Bocquet, Lyderic

    2015-05-07

    A challenge for the development of nanofluidics is to develop new instrumentation tools, able to probe the extremely small mass transport across individual nanochannels. Such tools are a prerequisite for the fundamental exploration of the breakdown of continuum transport in nanometric confinement. In this letter, we propose a novel method for the measurement of the hydrodynamic permeability of nanometric pores, by diverting the classical technique of Coulter counting to characterize a pressure-driven flow across an individual nanopore. Both the analysis of the translocation rate, as well as the detailed statistics of the dwell time of nanoparticles flowing across a single nanopore, allow us to evaluate the permeability of the system. We reach a sensitivity for the water flow down to a few femtoliters per second, which is more than two orders of magnitude better than state-of-the-art alternative methods.

  20. LEUCOAGGLUTINATION IN VITRO

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    Deepak Nayak

    2013-01-01

    Full Text Available ABSTRACT: Phenomenon such as cold agglutination of red blood cells and platelets are well recognized and extensively studied. Nevertheless, a ggregation of leukocytes in vitro is a rare phenomenon and may result in pseudoleukopenia. The ex act mechanism for this phenomenon has not been elucidated. We report one such rare cas e where a male patient with HIV presented with leucopenia. The analysis of ethylene diamine te traacetic acid (EDTA anticoagulated blood was done on Coulter LH 750 hematology analyzer. The Volume Conductivity Scatter (VCS parameters and histogram generated were inconclusive .The peripheral smear revealed small aggregates of leucocytes. The leucoaggregates were reversed on incubating the blood sample at 37 degree Celsius for 30 minutes. This suggests the role of a temperature dependent antibody causing clumping of leucocytes; possibly in conjuncti on with EDTA

  1. Performance of calibration standards for antigen quantitation with flow cytometry.

    Science.gov (United States)

    Lenkei, R; Gratama, J W; Rothe, G; Schmitz, G; D'hautcourt, J L; Arekrans, A; Mandy, F; Marti, G

    1998-10-01

    In the frame of the activities initiated by the Task Force for Antigen Quantitation of the European Working Group on Clinical Cell Analysis (EWGCCA), an experiment was conducted to evaluate microbead standards used for quantitative flow cytometry (QFCM). An unified window of analysis (UWA) was established on three different instruments (EPICS XL [Coulter Corporation, Miami, FL], FACScan and FACS Calibur [Becton Dickinson, San Jose, CA]) with QC3 microbeads (FCSC, PR). By using this defined fluorescence intensity scale, the performance of several monoclonal antibodies directed to CD3, CD4, and CD8 (conjugated and unconjugated), from three manufacturers (BDIS, Coulter [Immunotech], and DAKO) was tested. In addition, the QIFI system (DAKO) and QuantiBRITE (BDIS), and a method of relative fluorescence intensity (RFI, method of Giorgi), were compared. mAbs reacting with three more antigens, CD16, CD19, and CD38 were tested on the FACScan instrument. Quantitation was carried out using a single batch of cryopreserved peripheral blood leukocytes, and all tests were performed as single color analyses. Significant correlations were observed between the antibody-binding capacity (ABC) values of the same CD antigen measured with various calibrators and with antibodies differing in respect to vendor, labeling and possible epitope recognition. Despite the significant correlations, the ABC values of most monoclonal antibodies differed by 20-40% when determined by the different fluorochrome conjugates and different calibrators. The results of this study indicate that, at the present stage of QFCM consistent ABC values may be attained between laboratories provided that a specific calibration system is used including specific calibrators, reagents, and protocols.

  2. Evidence of a cold immunoglobulin M autoantibody against 78-kD platelet glycoprotein in a case of EDTA-dependent pseudothrombocytopenia.

    Science.gov (United States)

    De Caterina, M; Fratellanza, G; Grimaldi, E; Varriale, V; Scopacasa, F; Di Maro, G; Formisano, S

    1993-02-01

    Pseudothrombocytopenia is a phenomenon in which the electronic count shows spuriously low platelet counts in subjects with normal platelet levels. The mechanism of anticoagulant-dependent pseudothrombocytopenia appears to involve cold reactive agglutinins against platelet antigens. The authors report a case of EDTA-dependent pseudothrombocytopenia with evidence of a cold immunoglobulin M antibody against 78-kD platelet membrane glycoprotein (GP). Cell counts were performed by Coulter Counter S-Plus VI (Coulter, Hialeah, FL) in the following anticoagulants: EDTA, Na-citrate, and citrate-theophylline-adenosine-dipyridamole. Anti-platelet antibodies and platelet membrane GP antigens were assayed by an immunofluorescence technique as described by Van dem Borne in 1978. An immunoglobulin M/lambda anti-platelet antibody was found to react in serum as well as in plasma EDTA at room temperature, but not at 37 degrees C. This antibody appeared to be directed against GP78 membrane antigen because this antigen was not detectable by immunofluorescence in platelets collected in EDTA and Na-citrate anticoagulant, whereas a fluorescence signal was revealed in platelets collected in citrate-theophylline-adenosine-dipyridamole. This evidence was confirmed by platelet clumping inhibition tests in which target platelets were pretreated with anti-GP monoclonal antibodies. Clumping in the presence of pseudothrombocytopenia serum was inhibited by anti-GP78kD and anti-GPIIb/IIIa but not by anti-Ib. In this case, GP78 appears to be involved in platelet clumping, together with IIb/IIIa complex. The partial inhibition of the phenomenon observed in citrate-theophylline-adenosine-dipyridamole is probably related to a lower expression of the membrane antigens in platelets collected in this anticoagulant.

  3. Chemical characteristics of dissolved organic matter (DOM) in relation to heavy metal concentrations in soil water from boreal peatlands after clear-cut harvesting

    Science.gov (United States)

    Kiikkilä, O.; Nieminen, T.; Starr, M.; Ukonmaanaho, L.

    2012-04-01

    Boreal peatlands form an important terrestrial carbon reserve and are a major source of dissolved organic matter (DOM) to surface waters, particularly when disturbed through forestry practices such as draining or timber harvesting. Heavy metals show a strong affinity to organic matter and so, along with DOM, heavy metals can be mobilized and transported from the soil to surface waters and sediments where they may become toxic to aquatic organisms and pass up the food chain. The complexation of heavy metals with DOM can be expected to be related and determined by the chemical characteristics of DOM and oxidation/reducing conditions in the peat. We extracted interstitial water from peat samples and determined the concentrations of dissolved organic carbon (DOC), dissolved organic nitrogen (DON) and Al, Cu, Zn and Fe in various fractions of DOM isolated by adsorption properties (XAD-8 fractionation) and molecular-weight (ultrafiltration). The peat samples were taken from 0-30 and 30-50 cm depth in drained peatland catchments two years after whole-tree or stem-only clear-cut harvesting (Scots pine or Norway spruce) had been carried out. The samples from the upper layer had been subject to alternating saturation/aeration conditions while the deeper layer had been continuously under the water table. The fractionation of DOC and DON according to both adsorption properties and molecular-weight fractions clearly differed between the upper and lower peat layers. While the hydrophobic acid fraction contained proportionally more DOC and DON than the hydrophilic acid fraction in the upper peat layer the results were vice versa in the lower peat layer. High-molecular-weight compounds (> 100 kDa) were proportionally more abundant in the upper and low-molecular-weight compounds (< 1 kDa) in the lower peat layer. These differences are assumed to reflect differences in the aerobic/ anaerobic conditions and degree of decomposition between the two layers. The concentrations of Zn, Al

  4. Myxomycetes da Chapada do Araripe (Crato - CE, Brasil Myxomycetes from Chapada do Araripe (Crato, CE, Brazil

    Directory of Open Access Journals (Sweden)

    Laise de Holanda Cavalcanti

    1998-12-01

    Full Text Available Foi efetuado um levantamento dos Myxomyeetes ocorrentes em área de floresta no município do Crato, CE (7º13'53" S; 39º24'28'' W; Alt. 422m, assinalando-se as seguintes famílias e gêneros: Ceratiomyxaceae (Ceratiomyxa, 1 sp.; Cribrariaceae (Cribraria, lsp., Dictydium, 1 sp.; Didymiaceae (Didymium, 2sp.; Enteridiaceae (Dicrydiaethalium, 1sp.; Physaraceae (Badhamia, 2sp., Fuligo, 2sp., Physarum, 5sp.; Steinonitaceae (Comatricha, 3sp., Sfemonitis, 6sp.; Trichiaceae (Arcyria, 3sp., Hemitrichia, 2sp., Periclwena, 1 sp.- Constatou-se preferência das espécies para frutificar em troncos mortos de dicotiledôneas, seguindo-se restos de palmeiras e folhedo. O levantamento eleva para 30 o número de espécies referidas para o Ceará. Uma família e 19 espécies são novos registros para o Estado e Arcyria magna var. rosea Rex é nova referência para o Brasil. São apresentados comentários, chave de identificação e distribuição geográfica das espécies no Nordeste do Brasil.A survey on Myxomycetes was made in the woods of Crato Municipality. State of Ceará, Brazil (7º13'53" S; 39º24,28" W; Alt. 422m. when the following families and genera were registered: Ceratiomyxaceae (Ceratiomyxa, 1 sp.; Cribrariaceae (Cribraria, 1 sp., Dictydium. 1 sp.; Didymiaceae (Didymium, 2sp.; Enteridiaceae Dictydiaethalium, 1 sp.; Physaraceae (Badhamia. 2sp., Fuligo, 2sp., Physarum, 5sp.; Stemonitaceae Comatricha, 3sp., Stemonitis, 6sp.; Trichiaceae (Arcyria, 3sp., Hemitrichia, 2sp., Perichaena, lsp..The species sporulated mostly on dead dicotyledones wood, followed by monocotyledones (Arecaceae debris and litter. The survey raises the number of registers referred to the State of Ceará to 30 species. One family and 19 species are new records for that State while Arcyria magna var. rosea Rex represents a new record for Brazil. Key for the species, comments and geographical distribution in Northeast Brazil arc presented.

  5. Will more intensive forest harvesting for bioenergy cause unacceptable depletion of base cation pools - a modelling study using the Heureka decision support system

    Science.gov (United States)

    Futter, M. N.; Lucas, R. W.; Egnell, G.; Holmström, H.; Laudon, H.; Nilsson, U.; Oni, S. K.; Lämâs, T.

    2012-04-01

    Intensive forest harvesting has the potential to remove base cations (BC; Ca, K, Mg and Na) from ecosystems more rapidly than they can be replaced through mineral weathering. For this reason, whole tree harvesting (i.e. branches and needles harvested) for biofuel production in Sweden and elsewhere may not be ecologically sustainable. Under some circumstances, excessive BC removal may lead to re-acidification of soil and surface waters and a reduction of the growth potential in subsequent forest rotations. There is considerable uncertainty in all components of stand-scale BC mass balance estimates associated with forest harvests. Estimates of weathering rates from a single site can range over more than an order of magnitude, deposition estimates are often poorly constrained and tree element concentrations can show considerable variation. Despite these uncertainties, BC dynamics play a key role in forest management and planning. The Heureka decision support system has been developed in Sweden for multi-criteria analysis of forest management scenarios. Heureka can be used to estimate timber production and economic return under a series of user-specified constraints. Here, we present a model application based on Heureka, a database of tree element concentrations, published weathering rate estimates and long-term monitoring data to estimate BC budgets and their associated uncertainty under a series of forest harvest scenarios at the Strömsjöliden production park in northern Sweden. We evaluated BC budgets under four long term forest management scenarios associated with "business as usual", more intensive production, nature conservation and reindeer husbandry. Despite the large amount of uncertainty, a number of trends emerged. Nature conservation and reindeer husbandry scenarios were, in general, more sustainable than the other scenarios. Model results suggested that stem-only harvest could remove BC more rapidly than they could be replaced by weathering at some

  6. Construction of an experimental plot seeder of wheat planting and compare it by imported one

    Directory of Open Access Journals (Sweden)

    I Eskandari

    2016-09-01

    Full Text Available Introduction Researchers frequently include multiple cultivars and fertility levels in field experiments. Therefore, the experiments sowing operation must represent a considerable saving in time and labor, compared to hand sowing. Greater flexibility in experimental design and setup could be achieved by equipment that enables quick changes in the cultivar and fertilizer rates from one plot to the next. A satisfactory seed drill must distribute a given quantity of seed evenly over a predetermined length of coulter row, the coulters must be spaced at exact intervals and depth of sowing must be uniform. In a self-propelled type of plot seeder, no coulter should run in a wheel track as the compaction of the soil can cause observable differences in vigor between plants in such a row and those in un-compacted rows. The machine should sow in succession from a try in which a series of seed pocket separated clearly and must be put into distributer funnel by an assistant operator. The length of gap being varied according to the nature and purpose of the plot. The objectives of this experiment were 1- to design and construct a local self-propelled plot seeder and 2- To compare it with the imported (Wintersteiger plot seeder in cereal breeding programs. Materials and Methods A small-plot seeder was designed and constructed to meet this objective. The unit consists of the following basic components: a toolbar for pulling a set of six blade coulter, an air compressor for lifting and putting down the openers and metering transmission drive wheel, an operators chair and work rack, one belt seed distribution. A cone-celled and rotor seed distributor is used for seed distribution to the openers. The cone system is connected to the gearbox and allows for great flexibility in changing cultivars, crop species, and plot length. This is driven by the separate drive wheel. The cone-celled distributor sows all the seed of the sample in making one complete turn. The

  7. Comparability analysis between dry chemistry analyzer and wet chemistry analyzer%干化学与湿化学分析仪的可比性分析

    Institute of Scientific and Technical Information of China (English)

    李金密; 龚春梅; 何秀玲; 陈开春; 王丰

    2015-01-01

    目的:探讨不同生化分析仪16项常规生化指标测定结果的可比性。方法参照美国临床实验室标准化委员会(NCCLS)EP9‐A2文件,以贝克曼DXC800全自动生化分析仪为参比系统,以强生VITROS5600全自动生化免疫分析仪为待比系统,用患者新鲜血清分别在2台仪器上检测16项常规生化指标,检查离群点,计算相关系数和直线回归方程,评估2个系统的预期偏差,并以美国临床实验室改进修正案(CLIA′88)对室间评估的允许误差的1/2为判断依据,对强生VITROS5600干化学系统的临床可接受性进行评价。结果参比系统与待比系统具有良好的相关性,除丙氨酸氨基转移酶(ALT)、乳酸脱氢酶(LDH)、肌酐(Crea)及部份医学决定水平处天门冬氨酸氨基转移酶(AST)、总钙(Ca)、血清总蛋白(TP)测定结果的偏差外,其余指标测定结果的偏差临床均可接受。结论强生VITROS5600干化学分析仪与Beckman DXC800全自动生化分析仪的检测结果具有可比性,但ALT、AST、LDH、Ca、Crea、TP临床比对多水平处的偏差不可接受,仍有必要针对不同方法建立不同的参考区间。%Objective To investigate the comparability of the results of 16 routine biochemical indexes detected by different biochemical analyzers .Methods With the Beckman DXC800 automatic biochemical analyzer as the refer‐ence system and the VITROS5600 automatic biochemical immune analyzer as the compared system according to the EP9‐A2 ,guideline established by NCCLS .The 16 routine biochemical indexes were detected in serum of patients by the two instruments .The clinical acceptability of the VITROS5600 dry chemistry system was evaluated according to the judgment basis of 1/2 allowable error of the external quality assessment in CLIA′88 standard .Results The refer‐ence system and the compared system had better comparability .All tests showed good

  8. 长期低剂量慢性辐射特殊环境人员唾液pH值、葡萄糖与淀粉酶含量检测与分析%Testing and analysis on pH value, concentration of glucose and amylase of salivary in persons in special environment of long term low dose radiation

    Institute of Scientific and Technical Information of China (English)

    王军; 程硕; 安源; 李刚; 郭静; 李广文; 高宗科; 赵增强; 张燕; 王东平; 陈云; 王宇欣; 李翠霞

    2012-01-01

    Objective To analyze the pH value, content changes of glucose and amylase of salivary in long term low dose radiation on exposure personnel. Methods We used accurate pH test paper to test salivary pH and used Beckman SYNCHRON CX7 large full automatic biochemical analyzer from the United States of America for detecting saliva glucose (glucose oxidase method). and salivary amylase (Amylase) concentrations ( Winslow's method). Results pH value ranged from 5.0 to 7. 1 (6.16 ±0.52) , glucose ranged from 0.67 to 1.95 (1. 320 ±0. 334)mmol/L, amylase ranged from 396. 80 to 1 190.60 (770. 508 ±42. 021) U/L. Conclusion The effect of long term and low dose of radiation on pH value, concentration of glucose and amylase in salivary should not be ignored.%目的 探讨长期低剂量慢性辐射特殊环境人员唾液pH值、葡萄糖与淀粉酶含量变化.方法 检测唾液pH值用精密pH试纸测定;使用美国Beckman SYNCHRON CX7大型全自动生化分析仪检测唾液中的葡萄糖含量(葡萄糖氧化酶法)与唾液淀粉酶(Amylase)浓度(温斯罗法).结果 唾液pH值为5.0~7.1(6.16±0.52);唾液葡萄糖为0.67 ~ 1.95(1.320±0.334) mmol/L;唾液淀粉酶为396.80~1 190.60(770.508±42.021) U/L.结论 长期小剂量慢性电离辐射对唾液pH值、葡萄糖与淀粉酶含量的影响不容忽视.

  9. Índices eritrocitarios en la esferocitosis hereditaria Erythrocyte indexes in hereditary spherocytosis

    Directory of Open Access Journals (Sweden)

    Silvia Eandi Eberle

    2007-12-01

    Full Text Available La esferocitosis hereditaria es un grupo heterogéneo de desórdenes caracterizados por la variabilidad en la clínica, en los defectos proteicos del citoesqueleto eritrocitario y en el tipo de herencia. Se estudió la sensibilidad y especificidad de la concentración de hemoglobina corpuscular media (CHCM y el índice de amplitud de distribución eritrocitaria (ADE en el screening diagnóstico de la esferocitosis hereditaria. Noventa y cuatro pacientes fueron comparados con niños sanos de igual sexo y edad. Todos los índices se obtuvieron por impedancia eléctrica (autoanalizador hematológico Coulter JT. En los pacientes con esferocitosis hereditaria, la CHCM (35.67±1.33 g/dl y el ADE (20.60±4.5%, fueron significativamente más elevados que en el grupo control (CHCM 33.48±0.68 g/dl, p 0.000; ADE 13.22±0.9%, p 0.000. Con los valores de corte utilizados en nuestro laboratorio (CHCM ≥ 34.5 g/dl; ADE ≥ 14.5% ambos índices elevados mostraron una sensibilidad de 81% y una especificidad de 98.9% en el screening de esferocitosis hereditaria. La combinación de ambos índices es un excelente predictor para el diagnóstico de esferocitosis hereditaria.Hereditary spherocytosis is a group of heterogenous disorders characterized by variability in its clinical manifestations, membrane protein defects and inheritance. We analysed the sensitivity and specificity of mean corpuscular hemoglobin concentration (MCHC and red cell distribution width (RDW in the diagnostic screening of hereditary spherocytosis. Ninetyfour patients were compared to equal number of healthy, age-matched children. All indexes were derived from measurements obtained by aperture impedance (Coulter Counter Model JT. In patients with hereditary spherocytosis, MCHC (35.67±1.33 g/dl and RDW (20.60±4.5% were significantly higher than in normal control subjects (MCHC 33.48±0.68 g/dl, p: 0.000; RDW 13.22±0.9%, p: 0.000. By using a cutoff for the MCHC of 34.5 g/dl and for the RDW

  10. Color Parameters of the Chromascop Shade Guide

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    William J. O'Brien

    2012-12-01

    Full Text Available The purposes of this study are: (1 determine the color of the twenty shades in the Ivoclar’s Chromascop shade guide, (2 determine the color representation of the shade guide described as coverage error (CE, and (3 compare this shade guide with the Vita Classical and Bioform shade guides. The spectral data was collected using Beckman model DU reflectance spectrophotometer equipped with an integrating sphere. Commission International de l’Eclairage (CIE chromaticity coordinates were calculated using CIE illuminant C and 1931 observer data, then converted to CIE L*a*b* and Munsell notation. Each shade was spectrophotometrically compared to the published colors of 335 human teeth. The minimum CIE L*a*b* color difference was calculated for each tooth and the average of these color differences was defined as the CE. The measured colors of the Chromascop guide had a CIE L* range of 79.67 to 65.61, an a* range of -0.71 to 3.85, and a b* range of 14.58 to 27.69. The average CE of the Chromascop shade guide was 3.38. The Chromascop shade guide has similar colors and a CE compared with the Bioform and Vita Classical shade guides, but with some shades of higher red and yellow components.

  11. Amino acid contents and transport of fixed N in nodules of Leucaena leucocephala variety K-8

    Energy Technology Data Exchange (ETDEWEB)

    DuBois, J.D.

    1987-04-01

    Seedlings of Leucaena leucocephala var. K-8 were grown with a N-free fertilizer or fertilizer containing /sup 15/N-depleted (NH/sub 4/)/sub 2/SO/sub 4/ (0.01 atom /sup 15/N; 10 ppm). The nodules of 5 month old trees grown on N-free media were used for /sup 15/N-enriched treatment and as controls. Nodules from plants grown on /sup 15/N-depleted media were also used. Nodules were extracted with 0.5% aqueous toluene and aliquots were analyzed with a Beckman 120B Amino Acid Analyzer. Samples were separated into free ammonium, Asp-N, Glu-N, Asn and Gln amide- and amino-N, and remaining amino acids. Fractions were then analyzed for /sup 15/N content. Asn (27.3 umol/gfw) represented 56% of the total free amino acid pool in the nodules. Asn (amide-N and amino-N) also represented approximately 77% of the total N fixed during the one hour /sup 15/N-enriched N/sub 2/ and the /sup 15/N-depleted treatments. Based on these findings and the fact that the ureide fraction is barely detectable in the nodules (0.25 ..mu..mol/gfw), the authors considers L. leucocephala an amide transporter of fixed N. Additional information will be presented on the amino acid contents of tissues, as well as a time course of amino acid content from seed through nodulation.

  12. Biomedical optics centers: forty years of multidisciplinary clinical translation for improving human health

    Science.gov (United States)

    Tromberg, Bruce J.; Anderson, R. Rox; Birngruber, Reginald; Brinkmann, Ralf; Berns, Michael W.; Parrish, John A.; Apiou-Sbirlea, Gabriela

    2016-12-01

    Despite widespread government and public interest, there are significant barriers to translating basic science discoveries into clinical practice. Biophotonics and biomedical optics technologies can be used to overcome many of these hurdles, due, in part, to offering new portable, bedside, and accessible devices. The current JBO special issue highlights promising activities and examples of translational biophotonics from leading laboratories around the world. We identify common essential features of successful clinical translation by examining the origins and activities of three major international academic affiliated centers with beginnings traceable to the mid-late 1970s: The Wellman Center for Photomedicine (Mass General Hospital, USA), the Beckman Laser Institute and Medical Clinic (University of California, Irvine, USA), and the Medical Laser Center Lübeck at the University of Lübeck, Germany. Major factors driving the success of these programs include visionary founders and leadership, multidisciplinary research and training activities in light-based therapies and diagnostics, diverse funding portfolios, and a thriving entrepreneurial culture that tolerates risk. We provide a brief review of how these three programs emerged and highlight critical phases and lessons learned. Based on these observations, we identify pathways for encouraging the growth and formation of similar programs in order to more rapidly and effectively expand the impact of biophotonics and biomedical optics on human health.

  13. Stem cell-mediated osteogenesis: therapeutic potential for bone tissue engineering

    Directory of Open Access Journals (Sweden)

    Neman J

    2012-03-01

    Full Text Available Josh Neman1, Amanda Hambrecht2, Cherie Cadry3, Rahul Jandial11Department of Neurosurgery, Beckman Research Institute, City of Hope National Cancer Center, Duarte, 2Department of Biological Sciences, University of Southern California, Los Angeles, 3Department of Public Health Sciences, University of California, Irvine, California, USAAbstract: Intervertebral disc degeneration often requires bony spinal fusion for long-term relief. Current arthrodesis procedures use bone grafts from autogenous bone, allogenic backed bone, or synthetic materials. Autogenous bone grafts can result in donor site morbidity and pain at the donor site, while allogenic backed bone and synthetic materials have variable effectiveness. Given these limitations, researchers have focused on new treatments that will allow for safe and successful bone repair and regeneration. Mesenchymal stem cells have received attention for their ability to differentiate into osteoblasts, cells that synthesize new bone. With the recent advances in scaffold and biomaterial technology as well as stem cell manipulation and transplantation, stem cells and their scaffolds are uniquely positioned to bring about significant improvements in the treatment and outcomes of spinal fusion and other injuries.Keywords: mesenchymal stem cell, osteogenesis, scaffolds, bone morphogenetic protein, Wnt, autograft, osteoblast

  14. The role of peroxiredoxin II in chemoresistance of breast cancer cells

    Directory of Open Access Journals (Sweden)

    Wang T

    2014-05-01

    Full Text Available Tieli Wang,1 Anthony Joseph Gomez Diaz,1 Yun Yen2 1Department of Chemistry and Biochemistry, California State University Dominguez Hills, Carson, CA, USA; 2Department of Clinical and Molecular Pharmacology, Beckman Research Institute of City of Hope National Medical Center, Duarte, CA, USA Abstract: Peroxiredoxin (PrxII belongs to a family of redox-active proteins that use redox-sensitive cysteine in the active site to reduce peroxides. PrxII is induced by various oxidative stimuli and plays an important protective role against oxidative radical damage by reactive oxygen species. PrxII expression levels are correlated with resistance to radiation therapy or certain anti-cancer drugs in radioresistant breast cancer cells, glioblastomas, and head and neck cancer cells as well as in tissue isolated from head and neck patients who do not respond to radiation therapy. Small interfering RNA (siRNA that inhibits the PrxII gene expression has been shown to partially reverse the radioresistant phenotype in radiation resistant breast cancer cells and sensitizes glioma cells to oxidative stress, highlighting the potential clinical importance of PrxII in radiation resistance in cancer. This article focuses on the role that PrxII may play in chemoresistant breast cancer cells. Keywords: siRNA, redox, cysteine disulfide bridges, targeted therapy, reactive oxygen species

  15. Development and validation of an MEKC method for determination of nitrogen-containing drugs in pharmaceutical preparations.

    Science.gov (United States)

    Buiarelli, Francesca; Coccioli, Franco; Jasionowska, Renata; Terracciano, Alessandro

    2008-09-01

    A fast and accurate micellar electrokinetic capillary chromatography method was developed for quality control of pharmaceutical preparations containing cold remedies as acetaminophen, salicylamide, caffeine, phenylephrine, pseudoephedrine, norephedrine and chlorpheniramine. The method optimization was realized on a Beckman P/ACE System MDQ instrument. The baseline separation of seven analytes was performed in an uncoated fused silica capillary internal diameter (ID)=50 microm using tris-borate (20 mM, pH=8.5) containing sodium dodecyl sulphate 30 mM BGE. On line-UV detection at 214 nm was performed and the applied voltage was 10 kV. The operating temperature was 25 degrees C. After experimental conditions optimization, the proposed method was validated. The evaluated parameters were: precision of migration time and of corrected peak area ratio, linearity range, limit of detection, limit of quantification, accuracy (recovery), ruggedness and applicability. The method was then successfully applied for the analysis of three pharmaceutical preparations containing some of the analytes listed before.

  16. Angiotensin-converting enzyme inhibition studies by natural leech inhibitors by capillary electrophoresis and competition assay.

    Science.gov (United States)

    Deloffre, Laurence; Sautiere, Pierre-Eric; Huybrechts, Roger; Hens, Korneel; Vieau, Didier; Salzet, Michel

    2004-06-01

    A protocol to follow the processing of angiotensin I into angiotensin II by rabbit angiotensin-converting enzyme (ACE) and its inhibition by a novel natural antagonist, the leech osmoregulator factor (LORF) using capillary zonal electrophoresis is described. The experiment was carried out using the Beckman PACE system and steps were taken to determine (a) the migration profiles of angiotensin and its yielded peptides, (b) the minimal amount of angiotensin II detected, (c) the use of different electrolytes and (d) the concentration of inhibitor. We demonstrated that LORF (IPEPYVWD), a neuropeptide previously found in leech brain, is able to inhibit rabbit ACE with an IC(50) of 19.8 micro m. Interestingly, its cleavage product, IPEP exhibits an IC(50) of 11.5 micro m. A competition assay using p-benzoylglycylglycylglycine and insect ACE established that LORF and IPEP fragments are natural inhibitors for invertebrate ACE. Fifty-four percent of insect ACE activity is inhibited with 50 micro m IPEP and 35% inhibition with LORF (25 mm). Extending the peptide at both N- and C-terminus (GWEIPEPYVWDES) and the cleavage of IPEP in IP abolished the inhibitory activity of both peptides. Immunocytochemical data obtained with antisera raised against LORF and leech ACE showed a colocalization between the enzyme and its inhibitor in the same neurons. These results showed that capillary zonal electrophoresis is a useful technique for following enzymatic processes with small amounts of products and constitutes the first evidence of a natural ACE inhibitor in invertebrates.

  17. Amino acid composition of some Mexican foods.

    Science.gov (United States)

    Morales de León, Josefina; Camacho, M Elena; Bourges, Héctor

    2005-06-01

    Knowledge of the amino acid composition of foods is essential to calculate their chemical score, which is used to predict protein quality of foods and diets. Though amino acid composition of many foods is reasonably well established, better knowledge is needed on native foods consumed in different regions and countries. This paper presents the amino acid composition of different presentations of raw and processed foods produced and consumed in Mexico. The amino acid composition was determined using Beckman amino acid analyzers (models 116 and 6300). Tryptophan was determined using the Spies and Chambers method. Of the different foods analyzed, some comments are made on native or basic foods in Mexico: Spirulin, where lysine is the limiting amino acid, with a chemical score of 67%, is a good source of tryptophan (1.16g/16 gN); amaranth contains high levels of sulphur amino acids (4.09 to 5.34 g/16gN), with a protein content of 15 g/100g; and pulque, a Pre-Hispanic beverage that contains high levels of tryptophan (2.58 g/16 gN) and sulphur amino acids (2.72 g/16 gN). Finally, insects are good sources of sulphur amino acids and lysine.

  18. A Novel Method for Spectral Similarity Measure by Fusing Shape and Amplitude Features

    Directory of Open Access Journals (Sweden)

    J. G. Ding

    2015-12-01

    Full Text Available Spectral similarity measure is the basis of spectral information extraction. The description of spectral features is the key to spectral similarity measure. To express the spectral shape and amplitude features reasonably, this paper presents the definition of shape and amplitude feature vector, constructs the shape feature distance vector and amplitude feature distance vector, proposes the spectral similarity measure by fusing shape and amplitude features (SAF, and discloses the relationship of fusing SAF with Euclidean distance and spectral information divergence. Different measures were tested on the basis of United States Geological Survey (USGS mineral_beckman_430. Generally, measures by integrating SAF achieve the highest accuracy, followed by measures based on shape features and measures based on amplitude features. In measures by integrating SAF, fusing SAF shows the highest accuracy. Fusing SAF expresses the measured results with the inner product of shape and amplitude feature distance vectors, which integrate spectral shape and amplitude features well. Fusing SAF is superior to other similarity measures that integrate SAF, such as spectral similarity scale, spectral pan-similarity measure, and normalized spectral similarity score(NS3 .

  19. Cytotoxic effect of Erythroxylum suberosum combined with radiotherapy in head and neck cancer cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Macedo, Taysa B.C.; Torres, Hianne M.; Yamamoto-Silva, Fernanda Paula; Silva, Maria Alves G. [Universidade Federal de Goias (UFG), Goiania, GO (Brazil). Escola de Odontologia; Elias, Silvia T.; Silveira, Damaris; Magalhaes, Perola O.; Lofrano-Porto, Adriana; Guerra, Eliete N.S., E-mail: elieteneves@unb.br [Universidade de Brasilia (UnB), Brasilia, DF (Brazil). Faculdade de Ciencias da Saude

    2016-01-15

    The mouth and oropharynx cancer is the 6{sup th} most common type of cancer in the world. The treatment may involve surgery, chemotherapy and radiotherapy. More than 50% of drugs against cancer were isolated from natural sources, such as Catharanthus roseus and epipodophyllotoxin, isolated from Podophyllum. The biggest challenge is to maximize the control of the disease, while minimizing morbidity and toxicity to the surrounding normal tissues. The Erythroxylum suberosum is a common plant in the Brazilian Cerrado biome and is popularly known as 'cabelo-de-negro'. The objective of this study was to evaluate the cytotoxic activity of Erythroxylum suberosum plant extracts of the Brazilian Cerrado biome associated with radiotherapy in human cell lines of oral and hypopharynx carcinomas. Cells were treated with aqueous, ethanolic and hexanic extracts of Erythroxylum suberosum and irradiated at 4 Gy, 6 Gy and 8 Gy. Cytotoxicity was evaluated by MTT assay and the absorbance was measured at 570 nm in a Beckman Counter reader. Cisplatin, standard chemotherapy, was used as positive control. The use of Erythroxylum suberosum extracts showed a possible radiosensitizing effect in vitro for head and neck cancer. The cytotoxicity effect in the cell lines was not selective and it is very similar to the effect of standard chemotherapy. The aqueous extract of Erythroxylum suberosum, combined with radiotherapy was the most cytotoxic extract to oral and hypopharynx carcinomas. (author)

  20. Quality control for X-ray dosimetry with Fricke dosimeter

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Vivianne Lucia Bormann de [Centro Regional de Cincias Nucleares (CRCN-NE/CNEN-PE), Recife, PE (Brazil)]. E-mail: lsouza@cnen.gov.br; Austerlitz, C. [East Carolina University, NC (United States)]. E-mail: camposc@ecu.edu

    2007-07-01

    It is a well-established fact that the success of radiation therapy is dependent on the accurate dosimetry of the radiation beam used. This dosimetry must include equipment performance as well. A system for Fricke dosimetry was used for this purpose. The Fricke solution and a parallel ionization chamber (used for comparison) were irradiated with an X-ray beam from a Pantak X-ray equipment. The ionization chamber was positioned at a distance of 500 mm of the focal point, in the central axis of the X-ray field which is defined as 100x100 mm{sup 2} at the calibration distance. A plexiglas phantom appropriate to hold the polypropylene tube was used to irradiate the vials with the Fricke solution. The irradiated solutions were transferred directly from the polypropylene vials to 10 mm length cuvettes and the optical densities were measured in 304 nm using a Beckman Counter Model DU 640 spectrophotometer. Three institutions were visited to verify their procedures and two were in accordance with the lAEA recommended procedures for soft X-ray dosimetry. The results of the measurements performed in the institutions visited show that the Fricke dosimeter system developed can be used for quality control for soft X-ray equipment. (author)

  1. Biologically inspired binaural hearing aid algorithms: Design principles and effectiveness

    Science.gov (United States)

    Feng, Albert

    2002-05-01

    Despite rapid advances in the sophistication of hearing aid technology and microelectronics, listening in noise remains problematic for people with hearing impairment. To solve this problem two algorithms were designed for use in binaural hearing aid systems. The signal processing strategies are based on principles in auditory physiology and psychophysics: (a) the location/extraction (L/E) binaural computational scheme determines the directions of source locations and cancels noise by applying a simple subtraction method over every frequency band; and (b) the frequency-domain minimum-variance (FMV) scheme extracts a target sound from a known direction amidst multiple interfering sound sources. Both algorithms were evaluated using standard metrics such as signal-to-noise-ratio gain and articulation index. Results were compared with those from conventional adaptive beam-forming algorithms. In free-field tests with multiple interfering sound sources our algorithms performed better than conventional algorithms. Preliminary intelligibility and speech reception results in multitalker environments showed gains for every listener with normal or impaired hearing when the signals were processed in real time with the FMV binaural hearing aid algorithm. [Work supported by NIH-NIDCD Grant No. R21DC04840 and the Beckman Institute.

  2. Effect of training on the performance of blood glucose monitoring using a reagent strip (Glucoprofil).

    Science.gov (United States)

    Chantelau, E; Starostina, E G; Antsiferov, M B

    1990-11-01

    A relatively simple, new test-strip for blood-glucose monitoring, the Glucoprofil strip with a reaction-zone of film-foil, was evaluated by testing 100 different blood glucose concentrations between 2.1 and 29.7 mmol/l. The results were obtained visually before and after training to read the color changes of the strip according to the scale on the strip-container. Plasma glucose measurements with the Beckman Glucose-Analyzer were used for reference. The results show that the Glucoprofil-strip readings correlated well with the reference method (r = 0.97), thorough training provided. The mean deviation of the strip results was less than 1 mmol/l. Similar results were obtained using another blood glucose strip, the Haemoglukotest 20-800 R. Our study indicates that the performance of the Glucoprofil strip is satisfactory, and hence the strip may be useful for clinical purposes. Consistent with previous reports we could demonstrate that training improved the reading results of the Glucoprofil strip.

  3. Goeckerman's therapy for psoriasis with special reference to serum pentraxin 3 level

    Energy Technology Data Exchange (ETDEWEB)

    Ctirad, A.; Lenka, B.; David, P.; Zdenek, F.; Kveta, H.; Karel, E.; Jan, K. [Charles University Prague, Hradec Kralove (Czech Republic). University Hospital

    2008-10-15

    Goeckerman's therapy (GT) of psoriasis is based on daily application of pharmacy grade coal tar on affected skin with subsequent exposure to UV light. Pentraxin 3 (PTX3) is a newly identified acute phase reactant with non redundant functions in innate immunity. PTX3 has been shown to be a reliable prognostic marker in patients with various inflammatory disorders including rheumatoid arthritis, vasculitis, and psoriasis. The aim of this study was to evaluate the influence of Goeckerman's therapy of psoriasis on levels of two pentraxins: long pentraxin PTX3 and C reactive protein in 49 patients with chronic plaque psoriasis. CRP was assessed by immunonephelometry on IMMAGE 800 (Beckman, USA). PTX3 was detected using sandwich ELISA detection set (Alexis Biochemicals, Switzerland). The serum levels of both parameters (expressed as average {+-} 1 SD) were significantly diminished after GT. The level of PTX3 dropped from 1.92 {+-} 0.72 ng/ml before GT to 1.66 {+-} 0.58 ng/ml after GT (P = 0.0396) and the level of CRP fell from 4.64 {+-} 3.93 mg/l to 1.66 {+-} 0.58 mg/l (P {lt} 0.0001). Comparing to healthy controls, the serum levels of both parameters before GT were significantly higher than those found in healthy blood donors and remained significantly increased after GT. Increased serum concentrations of pentraxin 3 and CRP are alleviated by GT in patients with psoriasis.

  4. 红霉素药渣作家禽蛋白质饲料的营养价值评价%The valuation of nutrition value of erythromycin waste residue used as poultry feed

    Institute of Scientific and Technical Information of China (English)

    刘超; 南佳平; 闵育娜; 刘虎; 王小平

    2003-01-01

    用蛋白质自动定氮仪和Beckman-121MB型氨基酸分析仪测定红霉素药渣,粗蛋白质为18.38 %,10种必需氨基酸总含量为3.839 %,其中赖氨酸0.265 %、蛋+胱氨酸0.704 %、色氨酸0.347 %、苏氨酸0.213 %、亮氨酸0.690 %、缬氨酸0.343 %、异亮氨酸0.375 %、苯丙+酪氨酸0.400 %、组氨酸0.282 %、精氨酸0.220 %.真可利用蛋氨酸(0.53 %)与大豆饼(0.54 %)接近.以家禽理想蛋白质为模型计算必需氨基酸平衡指数(IEAA)是0.662~1.323,结合失衡度等指数的计算结果,证实红霉素药渣为家禽饲料的良好蛋白源.

  5. Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations

    Directory of Open Access Journals (Sweden)

    Marina Franco

    2012-01-01

    Full Text Available A competitive CZE method for quality control analysis of multivitamin preparations and veterinary products containing B-group vitamins was developed. Vitamins of interest are thiamine hydrochloride (B1, thiamine monophosphate chloride (B1a, riboflavine (B2, riboflavine-5′monophosphate (B2a, nicotinamide (B3, d-pantothenic acid calcium salt (B5, pyridoxine hydrochloride (B6, folic acid (B9, and 4-aminobenzoic acid (B10. These analytes were separated optimizing the experimental conditions in 20 mM tetraborate buffer pH=9.2 as a BGE (background electrolyte, on a Beckman P/ACE System MDQ instrument, using uncoated fused silica capillary. The effective capillary length was of 49.5 cm, I.D.=50 μm, the applied voltage 20 kV and the temperature 25∘C. Detection was performed by a diode array detector at 214 nm for all vitamins except B5 (190 nm and B2a (260 nm. Separation time was about 9 min. After experimental conditions optimization, the proposed method was validated. Precision of migration time and corrected peak area, linearity range, LOD and LOQ, accuracy (recovery, robustness, and ruggedness were evaluated for each analyte demonstrating the good reliability of the method. Analyses of the pharmaceutical real samples were performed and confirmed the versatility of this method.

  6. C-reactive protein as a risk factor for acute coronary syndrome

    Institute of Scientific and Technical Information of China (English)

    杨胜利; 何秉贤; 何作云; 张华; 何学兰; 张伟

    2002-01-01

    Objective We assessed thelevels of C-reactive protein ( CRP ) in patients with acute coronary syndrome (ACS) [including unstable angina pectoris (UAP), acute myocardial infarction (AMI) and sudden cardiac death (SCD) ] compared with non-ACS [including stable angina pectoris (SAP), old myocardial infarction (OMI) and healthy volunteers] and sought to test whether CRP are associated with clinical acute coronary syndrome. Methods Ultrasensitive immunoassay (rate nephelometry with the Beckman Array multitest immunoassay system) wasused to measure CRP levels in 91 patients with ACS(20 UAP, 71 AMI including 2 SCD) and non-ACS (34SAP, 25 patients with healing phase of AMI, 41 OMI and 94 control healthy subjects) Results CRP levels were higher in ACS group (18.50 + 23.98 mg/L [ SE 2.51, n = 91 ] ) compared with non - ACS group (3.89+7.14mg/L[SE0.51, n=194]) (P <0.01).Using Logistic Regression, CRP was a potent determinant of ACS ( OR = 1.65) Conclusion These results suggest that CRP has a strong association with ACS, and CRP is a risk factor of ACS.

  7. Roger Hayward and the Invention of the Two-Mirror Schmidt

    Science.gov (United States)

    Bell, T. E.

    2005-12-01

    Roger Hayward (1899-1979), now virtually unknown, was a multitalented architect, scientific illustrator, and optical inventor. Remembered primarily for illustrating Scientific American magazine's Amateur Scientist column between 1949 and 1974, he also illustrated more than a dozen textbooks in optics, physics, geology, oceanography, and chemistry, several of which became classics in their fields. He designed façades with astronomical themes for major buildings in Los Angeles, California, and sculpted mammoth, realistic models of the moon for Griffith Observatory, Adler Planetarium, and Disneyland. Throughout his life, he recreationally painted watercolors and oils that at least one critic likened to the work of John Singer Sargent. Hayward is least known as an optical designer, yet he made significant contributions to the DU spectrophotometer that established the multimillion-dollar company Beckman Instruments. During the pre-radar days of World War II at Mount Wilson Observatory, Hayward invented a classified Cassegrain version of the Schmidt telescope especially adapted for nighttime infrared aerial photography, plus extraordinarily simple machines that allowed inexperienced soldiers to grind, polish, and test accurate aspheric Schmidt correcting plates at speeds compatible with mass production - and later received U.S. patents for them all. This paper, drawn in part from unpublished letters between Hayward and Albert G. Ingalls, will feature little-known images of Hayward's work.

  8. A Retrospective Analysis of Urine Drugs of Abuse Immunoassay True Positive Rates at a National Reference Laboratory.

    Science.gov (United States)

    Johnson-Davis, Kamisha L; Sadler, Aaron J; Genzen, Jonathan R

    2016-03-01

    Urine drug screens are commonly performed to identify drug use or monitor adherence to drug therapy. The purpose of this retrospective study was to evaluate the true positive and false positive rates of one of our in-house urine drug screen panels. The urine drugs of abuse panel studied consists of screening by immunoassay then positive immunoassay results were confirmed by mass spectrometry. Reagents from Syva and Microgenics were used for the immunoassay screen. The screen was performed on a Beckman AU5810 random access automated clinical analyzer. The percent of true positives for each immunoassay was determined. Agreement with previously validated GC-MS or LC-MS-MS confirmatory methods was also evaluated. There were 8,825 de-identified screening results for each of the drugs in the panel, except for alcohol (N = 2,296). The percent of samples that screened positive were: 10.0% for amphetamine/methamphetamine/3,4-methylenedioxy-methamphetamine (MDMA), 12.8% for benzodiazepines, 43.7% for opiates (including oxycodone) and 20.3% for tetrahydrocannabinol (THC). The false positive rate for amphetamine/methamphetamine was ∼14%, ∼34% for opiates (excluding oxycodone), 25% for propoxyphene and 100% for phencyclidine and MDMA immunoassays. Based on the results from this retrospective study, the true positive rate for THC drug use among adults were similar to the rate of illicit drug use in young adults from the 2013 National Survey; however, our positivity rate for cocaine was higher than the National Survey.

  9. Evaluation of the ASTRA 4 new microprocessor-controlled electrolyte analyzer.

    Science.gov (United States)

    Hartmann, A E; Fillbach, J R

    1980-09-01

    A new programmable microprocessor-controlled multichannel chemistry analyzer, the Bechman ASTRA 4, was evaluated in an electrolyte configuration including sodium, potassium, chloride, and CO2. Sodium and potassium are analyzed with ion-selective electrodes, CO2 by differential rate pH measurement, and chloride by proportional coulometric titration, Day-to-day reproducibility studies yielded coefficients of variation of 0.8% for Na+, 1.2% for K+, 0.9% for Cl-, and 5.9% for CO2. Linearity was 100-200 mmol/l for Na+, 1.0-10.0 mmol/l for K+, 50-150 mmol/l for Cl+, and 5-50 mmol/l for CO2. There was excellent correlation between the ASTRA 4 and the comparison laboratory methodologies consisting of flame photometry, coulometry, and Beckman Chlorie/CO2 titrator. Performance of the ASTRA 4 was judged acceptable relative to requirements of medical usefulness. The instrument can be used as a routine electrolyte analyzer with a throughput of 60 samples an hour or as a stat analyzer with results obtained within 8.5 minutes. Each sample can be programmed for any combination of tests, and reagent is only consumed on those tests selected. Sample size is approximately 130 microliter for all four tests or reduced accordingly if fewer tests are selected.

  10. The significance of C-reactive protein levels in women with premature rupture of membranes.

    Science.gov (United States)

    Ismail, M A; Zinaman, M J; Lowensohn, R I; Moawad, A H

    1985-02-15

    In a prospective study of 100 patients with preterm premature rupture of membranes, clinical chorioamnionitis was present in 18 and histologic chorioamnionitis was present in 63. Patients who were managed conservatively for premature rupture of membranes were monitored by C-reactive protein determination, white blood cell and differential counts, maternal temperature, and fetal heart tone. C-reactive protein was measured nephelometrically (Immuno-chemistry Analyzer II, Beckman). Elevated C-reactive protein levels correlated well with both the pathologic and the clinical diagnosis of chorioamnionitis. Elevated C-reactive protein levels (at least 12 to 24 hours before delivery) were more sensitive than other standard laboratory or clinical tests in predicting chorioamnionitis both by clinical and pathologic criteria. When C-reactive protein values were normal, clinical chorioamnionitis was rarely found, whereas pathologically diagnosed chorioamnionitis was found half of the time. We conclude that although the C-reactive protein level is a very sensitive predictor of infectious morbidity in premature rupture of membranes, its specificity is not high.

  11. The determination of calcium in phosphate, carbonate, and silicate rocks by flame photometer

    Science.gov (United States)

    Kramer, Henry

    1956-01-01

    A method has been developed for the determination of calcium in phosphate, carbonate, and silicate rocks using the Beckman flame photometer, with photomultiplier attachement. The sample is dissolved in hydrofluoric, nitric, and perchloric acids, the hydrofluoric and nitric acids are expelled, a radiation buffer consisting of aluminum, magnesium, iron, sodium, potassium, phosphoric acid, and nitric acid is added, and the solution is atomized in an oxy-hydrogen flame with an instrument setting of 554 mµ. Measurements are made by comparison against calcium standards, prepared in the same manner, in the 0 to 50 ppm range. The suppression of calcium emission by aluminum and phosphate was overcome by the addition of a large excess of magnesium. This addition almost completely restores the standard curve obtained from a solution of calcium nitrate. Interference was noted when the iron concentration in the aspirated solution (including the iron from the buffer) exceeded 100 ppm iron. Other common rock-forming elements did not interfere. The results obtained by this procedure are within ± 2 percent of the calcium oxide values obtained by other methods in the range 1 to 95 percent calcium oxide. In the 0 to 1 percent calcium oxide range the method compares favorably with standard methods.

  12. Biological characterization of a novel and potent gonadotropin releasing hormone (GnRH) antagonist LXT-101

    Institute of Scientific and Technical Information of China (English)

    Xiao-liCHI; Wen-xiaZHOU; Jun-pingCHENG; Yong-xiangZHANG; Ke-liangLIU

    2005-01-01

    AIM The biological characterization of LXT-101 was investigated in vivo using intact male rats and nude mice bearing xenografts of LNCaP prostate cancer. The effect of LXT-101 on the proliferation of androgen -sensitive prostate cancer cell LNCaP and androgen-insensitive DU145 and PC-3M in vitro was also determined. METHODS Rats were injected subcutaneously with LXT-101 while control animals received only vehicle (5% mannitol). Blood samples were collected at different time after adminis-tration of LXT-101. The androgen-dependent LNCaP prostate cancer cells were grown, mixed with Matrigel and injected subcutaneously into nude mice. Experimental group received LXT-101 injectionfor up to 4 weeks. At the end point, blood samples were drawn and the excised tumors and sex organswere weighted. The serum testosterone was determined by specific immunochemiluminescence assay using kits produced by Beckman-Counter Co. The mRNA expressions of the genes of hormone receptor related to the gonadal axis were investigated by real-time RT-PCR technique. Cell viability was determined by MTT method.

  13. The value of Golgi protein 73 detection combined with alpha-fetoprotein,α-L-fucosidase,α-antitrypsin in the diagnosis of primary liver cancer%高尔基体蛋白73联合甲胎蛋白、α-L 岩藻糖苷酶、α-抗胰蛋白酶检测对原发性肝癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    黄华; 梁红梅; 胥慧; 周庭雄; 王建蔚

    2015-01-01

    Objective To study the diagnostic value of the golgi protein 73 (GP73) combined with alpha‐fetoprotein (AFP) ,α‐L‐fucosidase (AFU) andα‐antitrypsin(α‐AT) detection in primary hepatic carcinoma(PHC) .Methods 100 patients with PHC ,100 patients with hepatitis and cirrhosis ,and 100 healthy people hospitalized in the hospital from October 2011 to May 2013 were en‐rolled in the study .Serum AFP levels were detected by using Beckman DxI800 chemiluminescent analyzer ,serum AFU ,α‐AT levels were detected by using Beckman DxC800 automatic biochemical analyzer ,and GP73 levels were measured by using enzyme‐linked immunosorbent GP73 quantitative detection kits .Results In patients with PHC and patients with hepatitis and cirrhosis ,serum GP73 ,AFP ,AFU ,α‐AT levels were significantly higher than healthy people(P<0 .05) .In patients with PHC ,GP73 ,AFP ,AFU ,α‐AT levels were significantly higher than those in patients with hepatitis and cirrhosis(P<0 .05) .The sensitivities of single serum GP73 ,AFP ,AFU ,α‐AT detection were 75% ,72% ,23% ,67% respectively and specificity were 95% ,95% ,100% ,97% respective‐ly .The sensitivity and specificity of the 4 tumor markers′combined detection were 99% and 100% .The sensitivity of combined de‐tection was significantly different from single detection(P<0 .05) .Conclusion GP73 ,AFP ,AFU ,α‐AT may improve the diagnostic efficiency of primary liver cancer .%目的:研究高尔基体蛋白73(GP73)联合甲胎蛋白(AFP)、α‐L岩藻糖苷酶(AFU)、α‐抗胰蛋白酶(α‐AT)检测对原发性肝癌诊断的价值。方法选取2011年10月至2013年5月在该院住院及就诊的原发性肝癌、肝炎和肝硬化患者及健康体检者各100例作为研究对象,使用Beckman DxI800化学发光分析仪检测血清AFP水平;用Beckman DxC800全自动生化分析仪检测血清AFU、α‐AT水平;用GP73酶联免疫定量检测试剂盒测定 GP73水平。结果原发性

  14. Smoking cessation: an application of theory of planned behavior to understanding progress through stages of change.

    Science.gov (United States)

    Bledsoe, Linda K

    2006-07-01

    The purpose of this research was to investigate variables relevant to smoking cessation early in the process of change through an application of the Theory of Planned Behavior [Ajzen, I. (1985). From intentions to actions: A theory of planned behavior. In J. Kuhl and J. Beckman (Eds). Action-control: From cognition to behavior (pp.11-39). Heidelberg: Springer.] to the temporal structure provided by the Transtheoretical Model. Study 1 was a preliminary elicitation study (n=68) conducted to ground the concepts used in the model testing in Study 2 [Ajzen, I., Fishbein, M. (1980). Understanding attitudes and predicting social behavior, Englewood Cliffs, NJ: Prentice-Hall.]. Study 2 tested the proposed model fit with data from a sample of 230 adult smokers. Structural equation modeling did not support the Theory of Planned Behavior as a model of motivation for progress through the stages of change and highlighted measurement issues with perceived behavioral control. A modified model using the Theory of Reasoned Action provided a good fit to the data, accounting for approximately 64% of the variance in intention to quit smoking and stage of change. This research addresses the need for a more complete theoretical rationale for progress through stages of change.

  15. An application of the theory of planned behavior to examine the impact of classroom inclusion on elementary school students.

    Science.gov (United States)

    Campbell, Michael

    2010-05-01

    Classroom inclusion serves as the most discussed service delivery model in the debate over the most appropriate way to provide education for students with disabilities. Integrating students with disabilities with nondisabled peers may increase attitudes of acceptance, but the literature also indicates that placement alone does not yield an increase in interaction between these two groups of peers (Brinker & Thorpe, 1986; Fryexe & Kennedy, 1995; Kennedy, Shulka, & Fryxell, 1997). This study investigated the impact of classroom inclusion on nondisabled students. Using survey research methods and guided by the Theory of Planned Behavior (Aizen, I. [1985]. From intentions to actions: A theory of planned behavior. In J. Kuhl & J. Beckman [Eds.], Action-control: From cognition to behavior [pp. 11-39]. Heidelberg: Springer), 593 responses were obtained from a convenience sample of 936 third, fourth, and fifth grade students and their parents from 52 classrooms spread across six different schools. Survey results were also collected from these students' parents and their teachers and used to add a richer depth to the data analysis. Implications for policy and practice are drawn. Limitations and recommendations for future research are also indicated.

  16. Comparison and bias analysis of BUN, Cr and UA between two different biochemical analysis systems%血尿素氮、肌酐及尿酸在两种生化分析系统的比对和偏倚评估

    Institute of Scientific and Technical Information of China (English)

    吕慧; 赖战峰; 李海炜; 林丽文; 秦雪; 李山

    2012-01-01

    Objective To discuss the comparability of blood urea nitrogen (BUN),creatinine (Cr),uric acid (UA) results and provide basis for result concordance in different biochemical systems.Methods The within-run precision and the between-day precision for the median and high values of quality control materials were tested in Beckman LX-20 and Hitachi 7600 chemistry analyzer respectively,and the evaluation criteria were according to the precision requirement of manufacturer and our laboratory.According to American Clinical and Laboratory Standards Institute (CLSI) document EP9-A2,serum BUN,Cr,UA from 40 patients were detected by Hitachi 7600 chemistry analyzer as experimental method and by Beckman LX-20 chemistry analyzer as comparative method.The relative bias ( SE%) between experimental method and comparative method was calculated and the concordance of biochemical analysis system was determined according to EQA requirements and acceptable performance criteria Results The between-run and between-day precision coefficients of variation for BUN,Cr and UA in two biochemical analysis systems were lower than the precision requirement of manufacturer and our laboratory.In Hitachi 7600 chemistry analyzer,the SE% for BUN at three levels of medical decision were 9.4%,2.0% and 0.8%,the SE% for Cr were 7.3%,3.8% and 2.8%,the SE% for UA were 7.9%,1.7% and 1.2%.Two biochemical analysis systems were not comparable in BUN analysis because SE% of serum BUN levels detected by Hitachi 7600 at low medical decision level exceeded the specified range.The remaining items between two biochemical analysis systems were concordant.Conclusion While the same test is detected by more than two systems in one laboratory,it is necessary to do method comparison and bias evaluation for concordance in order to ensure the comparability among test results.%目的 探讨不同的生化检测系统间血尿素氮(BUN)、肌酐(Cr)、尿酸(UA)三项指标测定结果的可比性,

  17. Axon clinical chemistry analyzer evaluated according to ECCLS protocol.

    Science.gov (United States)

    Brenna, S; Prencipe, L

    1992-10-01

    We assessed the analytical performance of the Axon system (Bayer Diagnostici), according to the European Committee for Clinical Laboratory Standards guidelines, for assay of 12 analytes: cholesterol, creatinine, glucose, total protein, urea, uric acid, alkaline phosphatase, alpha-amylase, aspartate aminotransferase, creatine kinase, sodium, and potassium. The field evaluation lasted approximately 5 months and involved the collection of approximately 10,000 data points with the Axon. The following results were obtained: The highest CVs for controls and human sera at different concentration/activity values were 2.2% for within-run imprecision (n = 60; 3 days, pooled estimate) and 3.5% for the between-day imprecision (n = 20 days). Close correlation was found with results for patients' specimens assayed with comparative instruments (Hitachi 717 for substrates and enzymes, Beckman Synchron EL/E4A for electrolytes). No drift was observed during 8 h of operation. The linearity range was broad, sometimes exceeding the manufacturer's claims. No sample-, reagent-, or cuvette-related carryover was found. Measurement of control sera gave results within +/- 5% of the assigned values. We conclude that good reliability and practicability make the Axon system suitable for laboratories with various needs.

  18. Antimicrobial activity of tetraacetylethylenediamine-sodium perborate versus sodium hypochlorite against Enterococcus faecalis

    Science.gov (United States)

    Shakouie, Sahar; Salem Milani, Amin; Eskandarnejad, Mahsa; Rahimi, Saeed; Froughreyhani, Mohammad; Galedar, Saeede; Ranjbar, Ehsan

    2016-01-01

    Background. This study evaluated the antimicrobial activity of Tetraacetylethylenediamine-sodium perborate (TAED-SP) in comparison to 2.5% and 5% sodium hypochlorite (NaOCl) against Enterococcus faecalis. Methods. A standard suspension of E. faecalis was inoculated on 60 plates containing Mueller-Hinton agar culture medium. Four sterile disks of Beckman filtration paper were placed on each plate. TAED-SP, 5% and 2.5% NaOCl were placed on three disks. Sterile physiologic saline was placed on the fourth disk as negative control. After 24-hour incubation, the diameter of the inhibition zone around the disks was measured using a transparent ruler. One-way Analysis of Variance (ANOVA) was used to compare the mean zone of microbial growth in the groups. P-values less than 0.05 were considered statistically significant. Results. There was a significant difference in the diameter of the inhibition zones between groups (P faecalis; however, TAED-SP has a greater antibacterial effect compared to 2.5% NaOCl. PMID:27092214

  19. ACCESS化学发光仪节省成本的几种方法探讨

    Institute of Scientific and Technical Information of China (English)

    吴敏校; 郑长业; 陈宏斌

    2008-01-01

    ACCESS全自动微粒子化学发光免疫分析仪由美国BECKMAN COULTE公司和法国PASTURE研究院合作设计生产,具有高灵敏度、高准确度、检测速度快、试剂稳定时间长等优点,可测定内分泌激素、肿瘤标志物、病毒标志物、心血管疾病标志物、治疗药物浓度和贫血等项目。它拓展了临床免疫检测范围,实现了临床免疫检测全自动化,但其试剂不开放,检测成本高,也给各用户造成成本压力,如何在保证检测质量的情况下降低成本,成为用户关心的重要问题。我们在几年的实际工作中,经过结果观察,总结出以下几种方法可以降低成本,且效果满意,现介绍如下:

  20. Failure rate analysis using GLIMMIX

    Energy Technology Data Exchange (ETDEWEB)

    Moore, L.M.; Hemphill, G.M.; Martz, H.F.

    1998-12-01

    This paper illustrates use of a recently developed SAS macro, GLIMMIX, for implementing an analysis suggested by Wolfinger and O`Connell (1993) in modeling failure count data with random as well as fixed factor effects. Interest in this software tool arose from consideration of modernizing the Failure Rate Analysis Code (FRAC), developed at Los Alamos National Laboratory in the early 1980`s by Martz, Beckman and McInteer (1982). FRAC is a FORTRAN program developed to analyze Poisson distributed failure count data as a log-linear model, possibly with random as well as fixed effects. These statistical modeling assumptions are a special case of generalized linear mixed models, identified as GLMM in the current statistics literature. In the nearly 15 years since FRAC was developed, there have been considerable advances in computing capability, statistical methodology and available statistical software tools allowing worthwhile consideration of the tasks of modernizing FRAC. In this paper, the approaches to GLMM estimation implemented in GLIMMIX and in FRAC are described and a comparison of results for the two approaches is made with data on catastrophic time-dependent pump failures from a report by Martz and Whiteman (1984). Additionally, statistical and graphical model diagnostics are suggested and illustrated with the GLIMMIX analysis results.

  1. 某部新兵唾液pH值、葡萄糖与淀粉酶含量检测与分析%Testing and Analysis on pH value, Concentration of Glucose and Amylase of Salivary in Recruit

    Institute of Scientific and Technical Information of China (English)

    王军; 郭静; 李刚; 李广文; 张燕; 赵增强; 王东平; 王宇欣; 李翠霞; 程硕; 安源

    2012-01-01

    目的 分析某部新兵唾液pH值、葡萄糖与淀粉酶含量的特点.方法 收集某部67名新兵的唾液样品,用精密pH试纸测定唾液pH值;使用美国Beckman SYNCHRON CX7大型全自动生化分析仪检测唾液中的葡萄糖含量与唾液淀粉酶浓度.结果 唾液pH值为6.87±0.34,唾液葡萄糖浓度为(0.996±0.191)mmol/L,唾液淀粉酶浓度为(1213.104±72.125)U/L.结论 本组结果可以为建立我军健康人群唾液pH值、葡萄糖与淀粉酶含量标准提供参考,同时也可为临床诊断提供参考.

  2. Blood flow dynamics after laser therapy of port wine stain birthmarks

    Science.gov (United States)

    Huang, Yu-Chih; Tran, Nadia; Ross, E. Victor; Shumaker, Peter R.; Nelson, J. Stuart; Kelly, Kristen; Choi, Bernard

    2009-02-01

    During laser therapy of port wine stain (PWS) birthmarks, regions of persistent perfusion may exist. We hypothesize that such regions, which are not readily visible, exist even during laser surgery performed by highly experienced clinicians. The objective of this study was to use objective feedback to assess the acute vascular response to laser therapy. We have developed a clinic-friendly laser speckle imaging (LSI) instrument to provide the clinician with real-time images of blood flow during laser therapy. We acquired images from patients undergoing laser therapy of PWS birthmarks at Scripps Clinic and Beckman Laser Institute and Medical Clinic. We extracted blood flow maps from the acquired imaging data. Collectively, we have observed two regimes of patient response to therapy: 1) an immediate increase in perfusion within minutes after laser therapy; and 2) an overall decrease in blood perfusion approximately one hour after laser therapy, with distinct regions of persistent perfusion apparent in the majority of post-treatment blood-flow images. A comparison of blood flow in PWS and adjacent normal skin demonstrated that PWS blood flow can be greater than or sometimes equivalent to that of normal skin. Regions of persistent perfusion frequently exist immediately after laser therapy of PWS birthmarks. Existence of these regions may be correlated to the need for multiple treatment sessions to improve substantially PWS skin appearance. With the use of intraoperative LSI, immediate retreatment of these regions may improve the outcome of each session.

  3. Proxies and measurement techniques for mineral dust in Antarctic ice cores.

    Science.gov (United States)

    Ruth, Urs; Barbante, Carlo; Bigler, Matthias; Delmonte, Barbara; Fischer, Hubertus; Gabrielli, Paolo; Gaspari, Vania; Kaufmann, Patrik; Lambert, Fabrice; Maggi, Valter; Marino, Federica; Petit, Jean-Robert; Udisti, Roberto; Wagenbach, Dietmar; Wegner, Anna; Wolff, Eric W

    2008-08-01

    To improve quantitative interpretation of ice core aeolian dust records, a systematic methodological comparison was made. This involved methods for water-insoluble particle counting (Coulter counter and laser-sensing particle detector), soluble ion analysis (ion chromatography and continuous flow analysis), elemental analysis (inductively coupled plasma mass spectroscopy at pH 1 and after full acid digestion), and water-insoluble elemental analysis (proton induced X-ray emission). Antarctic ice core samples covering the last deglaciation from the EPICA Dome C (EDC) and the EPICA Dronning Maud Land (EDML) cores were used. All methods correlate very well among each other, but the ratios of glacial age to Holocene concentrations, which are typically a factor approximately 100, differ between the methods by up to a factor of 2 with insoluble particles showing the largest variability. The recovery of ICP-MS measurements depends on the digestion method and is differentfor different elements and during different climatic periods. EDC and EDML samples have similar dust composition, which suggests a common dust source or a common mixture of sources for the two sites. The analyzed samples further reveal a change of dust composition during the last deglaciation.

  4. Involvement of the thymus and cellular immune system in craniofacial malformation syndromes.

    Science.gov (United States)

    Scheuerle, A E; Good, R A; Habal, M B

    1990-04-01

    Craniofacial structures, the aortic arch, thymus, and parathyroid glands all arise from the embryologic pharyngeal pouches, and DiGeorge and Job craniofacial malformation syndromes have defined immunologic deficiencies. The question addressed by this study is whether patients with other pharyngeal pouch malformations could also have immunologic abnormalities. Twelve patients, 4 female and 8 male, were selected at random from the Tampa Bay Craniofacial Center. Their diagnoses included: cleft lip/cleft palate, hemifacial microsomia/Goldenhar syndrome, Treacher-Collins syndrome, craniofacial hemangiomata, cranio-synostosis syndromes, and Tessier 13 cleft. Fresh blood samples were analyzed against age-matched controls for immunoglobin number, using immunoelectrophoresis, T-cell, B-cell, and natural killer cell quantity via Coulter counter and monoclonal antibody labeling, as well as lymphocyte stimulation and response functions with phytohemagglutinin, concanavalin A, pokeweed mitogen, and Staphylococcus aureus mitogens. All patients studied had some abnormality of their immune systems. Seven had specific T-cell abnormalities and three patients had abnormalities in all three categories studied. This indicates that patients with any pharyngeal pouch malformation may have an abnormality of the immune system.

  5. Struvite crystalluria and urolithiasis in cross Labradors.

    Science.gov (United States)

    Rodgers, A L; Irving, R A; Mezzabotta, M; Benloulou, P

    1986-01-01

    Recurrent struvite crystalluria and urolithiasis in a cross-Labrador bitch was studied using a combined Coulter-Counter and scanning electron microscope (SEM) approach. Staphylococcus bacteria were cultured from the patient's urine as well as from the calculi themselves. Urine samples were subjected to particle counting and sizing during active and non-active periods of stone formation. Size distribution curves so obtained were identical as were those derived from sterile and non-sterile specimens. These showed a peak incidence at a diameter of 5 microns. Particle sizes for 6 controls were also determined and showed an even distribution over a much wider range with small peaks occurring at 3, 10, and 20 microns diameters. SEM studies of urine sediments revealed the presence of struvite crystals in all the controls as well as in the stone-former. These occurred in a variety of shapes and sizes but were generally larger in the controls. SEM also revealed intimate admixtures of struvite and apatite in calculi surgically removed from the patient. The results of this study indicate that crystal numbers are of greater significance than crystal size. It is also suggested that Cross-Labradors may be unusually predisposed to struvite crystalluria. The repeated recurrence of struvite urolithiasis in the subject indicates a possible inherent physiological malfunction in the animal's ability to cope with this crystalluria. The absence of a nucleation inhibitor in the stone-former's urine is also postulated.

  6. Microfluidic CODES: a scalable multiplexed electronic sensor for orthogonal detection of particles in microfluidic channels.

    Science.gov (United States)

    Liu, Ruxiu; Wang, Ningquan; Kamili, Farhan; Sarioglu, A Fatih

    2016-04-21

    Numerous biophysical and biochemical assays rely on spatial manipulation of particles/cells as they are processed on lab-on-a-chip devices. Analysis of spatially distributed particles on these devices typically requires microscopy negating the cost and size advantages of microfluidic assays. In this paper, we introduce a scalable electronic sensor technology, called microfluidic CODES, that utilizes resistive pulse sensing to orthogonally detect particles in multiple microfluidic channels from a single electrical output. Combining the techniques from telecommunications and microfluidics, we route three coplanar electrodes on a glass substrate to create multiple Coulter counters producing distinct orthogonal digital codes when they detect particles. We specifically design a digital code set using the mathematical principles of Code Division Multiple Access (CDMA) telecommunication networks and can decode signals from different microfluidic channels with >90% accuracy through computation even if these signals overlap. As a proof of principle, we use this technology to detect human ovarian cancer cells in four different microfluidic channels fabricated using soft lithography. Microfluidic CODES offers a simple, all-electronic interface that is well suited to create integrated, low-cost lab-on-a-chip devices for cell- or particle-based assays in resource-limited settings.

  7. Rapid, Precise, and Accurate Counts of Symbiodinium Cells Using the Guava Flow Cytometer, and a Comparison to Other Methods.

    Science.gov (United States)

    Krediet, Cory J; DeNofrio, Jan C; Caruso, Carlo; Burriesci, Matthew S; Cella, Kristen; Pringle, John R

    2015-01-01

    In studies of both the establishment and breakdown of cnidarian-dinoflagellate symbiosis, it is often necessary to determine the number of Symbiodinium cells relative to the quantity of host tissue. Ideally, the methods used should be rapid, precise, and accurate. In this study, we systematically evaluated methods for sample preparation and storage and the counting of algal cells using the hemocytometer, a custom image-analysis program for automated counting of the fluorescent algal cells, the Coulter Counter, or the Millipore Guava flow-cytometer. We found that although other methods may have value in particular applications, for most purposes, the Guava flow cytometer provided by far the best combination of precision, accuracy, and efficient use of investigator time (due to the instrument's automated sample handling), while also allowing counts of algal numbers over a wide range and in small volumes of tissue homogenate. We also found that either of two assays of total homogenate protein provided a precise and seemingly accurate basis for normalization of algal counts to the total amount of holobiont tissue.

  8. Rapid, Precise, and Accurate Counts of Symbiodinium Cells Using the Guava Flow Cytometer, and a Comparison to Other Methods.

    Directory of Open Access Journals (Sweden)

    Cory J Krediet

    Full Text Available In studies of both the establishment and breakdown of cnidarian-dinoflagellate symbiosis, it is often necessary to determine the number of Symbiodinium cells relative to the quantity of host tissue. Ideally, the methods used should be rapid, precise, and accurate. In this study, we systematically evaluated methods for sample preparation and storage and the counting of algal cells using the hemocytometer, a custom image-analysis program for automated counting of the fluorescent algal cells, the Coulter Counter, or the Millipore Guava flow-cytometer. We found that although other methods may have value in particular applications, for most purposes, the Guava flow cytometer provided by far the best combination of precision, accuracy, and efficient use of investigator time (due to the instrument's automated sample handling, while also allowing counts of algal numbers over a wide range and in small volumes of tissue homogenate. We also found that either of two assays of total homogenate protein provided a precise and seemingly accurate basis for normalization of algal counts to the total amount of holobiont tissue.

  9. Prehistoric peyote use: alkaloid analysis and radiocarbon dating of archaeological specimens of Lophophora from Texas.

    Science.gov (United States)

    El-Seedi, Hesham R; De Smet, Peter A G M; Beck, Olof; Possnert, Göran; Bruhn, Jan G

    2005-10-01

    Two archaeological specimens of peyote buttons, i.e. dried tops of the cactus Lophophora williamsii (Lem.) Coulter, from the collection of the Witte Museum in San Antonio, was subjected to radiocarbon dating and alkaloid analysis. The samples were presumably found in Shumla Cave No. 5 on the Rio Grande, Texas. Radiocarbon dating shows that the calibrated 14C age of the weighted mean of the two individual dated samples corresponds to the calendric time interval 3780-3660 BC (one sigma significance). Alkaloid extraction yielded approximately 2% of alkaloids. Analysis with thin-layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS) led to the identification of mescaline in both samples. No other peyote alkaloids could be identified. The two peyote samples appear to be the oldest plant drug ever to yield a major bioactive compound upon chemical analysis. The identification of mescaline strengthens the evidence that native North Americans recognized the psychotropic properties of peyote as long as 5700 years ago.

  10. Peyote identification on the basis of differences in morphology, mescaline content, and trnL/trnF sequence between Lophophora williamsii and L. diffusa.

    Science.gov (United States)

    Aragane, Masako; Sasaki, Yohei; Nakajima, Jun'ichi; Fukumori, Nobutaka; Yoshizawa, Masao; Suzuki, Yukiko; Kitagawa, Shigemi; Mori, Ken'ichiro; Ogino, Shuzo; Yasuda, Ichiro; Nagumo, Seiji

    2011-01-01

    Genus Lophophora (Cactaceae) has two species: Lophophora williamsii Coulter, which is called peyote, and L. diffusa Bravo. Although it was reported that L. williamsii contained mescaline and L. diffusa did not, we found L. williamsii specimens that did not contain mescaline. This finding indicated that the two species could not be differentiated in terms of mescaline content. Moreover, the relationship between mescaline content and morphology of the two species is also unknown. In this study, we attempted to clarify the difference in morphology, mescaline content, and DNA alignment of the chloroplast trnL/trnF region between L. williamsii and L. diffusa. As a result, L. williamsii specimens were classified into two groups. Group 1 had small protuberances on the epidermis, contained mescaline, and the analyzed region on the trnL/trnF sequence was 881 base pairs (bp) long in all except one (877 bp). Group 2 had large protuberances on the epidermis, did not contain mescaline, and the analyzed region was 893 bp long. On the other hand, L. diffusa had medium-sized protuberances on the epidermis, did not contain mescaline, and the analyzed region was 903 bp long. Also investigated was the potential application of the PCR-restriction fragment length polymorphism (RFLP) method as a means of identification based on the trnL/trnF sequence. By applying the PCR-RFLP method, the two species could be distinguished and L. williamsii specimens could be differentiated into group 1 and group 2.

  11. [Studies of platelet aggregation in six cases of EDTA-dependent pseudothrombocytopenia].

    Science.gov (United States)

    Shimasaki, A; Kato, T; Ozaki, Y

    1994-06-01

    Peripheral blood count was performed by a Coulter Model S Plus STKR on six pseudothrombocytopenia patients (age: 16-70, 2 men and 4 women) using three different anticoagulants. Treatment with ethylene diamine tetraacetate (EDTA, 1 mg/ml) or sodium heparin (25 U/ml) aggregated platelets, but sodium citrate (3.8%, 1:9) had no effect. Smear examination revealed much platelet clumping but the satellite phenomenon was not present. No specific pattern was elucidated concerning cell size distribution curves between treatment by EDTA and heparin. Theophylline (10 mg/ml) and prostaglandin I2 (1 microM) inhibited EDTA-induced platelet aggregation but aspirin (1.8 mM) did not. On the other hand, these three substances inhibited heparin-induced platelet aggregation. These findings, taken together, suggested that EDTA and heparin initiated platelet activation and EDTA-induced platelet aggregation might be a process unrelated to thromboxane A2 production. Heparin may not be a suitable anticoagulant since it aggregates platelets of some healthy individuals.

  12. Cytotoxic effect of the pentacyclic oxindole alkaloid mitraphylline isolated from Uncaria tomentosa bark on human Ewing's sarcoma and breast cancer cell lines.

    Science.gov (United States)

    García Giménez, Dolores; García Prado, Elena; Sáenz Rodríguez, Teresa; Fernández Arche, Angeles; De la Puerta, Rocío

    2010-02-01

    Preparations from Uncaria tomentosa, a South American Rubiaceae, have been used in the Peruvian traditional medicine for the treatment of infective, inflammatory and tumoral processes. In this study, the pentacyclic oxindole alkaloid mitraphylline was isolated from the dried inner bark of this plant species, and its structure elucidated by analysis of NMR spectroscopic data. Mitraphylline was differentially identified from its stereoisomeric pair isomitraphylline by (15)N-NMR. Its antiproliferative and cytotoxic effects have been tested on human Ewing's sarcoma MHH-ES-1 and breast cancer MT-3 cell lines, using cyclophosphamide and vincristine as reference controls. A Coulter counter was used to determine viable cell numbers, followed by the application of the tetrazolium compound MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy phenyl)-2-(4-sulfophenyl)-2H-tetrazolium] an inner salt. A colorimetric method was employed to evaluate cell viability in this cytotoxic assay. Micromolar concentrations of mitraphylline (5 microM to 40 microM) inhibited the growth of both cell lines in a dose-dependent manner. The IC (50) +/- SE values were 17.15 +/- 0.82 microM for MHH-ES-1 and 11.80 +/- 1.03 microM for MT-3 for 30 hours, smaller than those obtained for the reference compounds. This action suggests that the pentacyclic oxindole alkaloid mitraphylline might be a new promising agent in the treatment of both human sarcoma and breast cancer.

  13. Bromelain proteases reduce human platelet aggregation in vitro, adhesion to bovine endothelial cells and thrombus formation in rat vessels in vivo.

    Science.gov (United States)

    Metzig, C; Grabowska, E; Eckert, K; Rehse, K; Maurer, H R

    1999-01-01

    The thiol protease, bromelain, an extract from pineapple stem, was suggested to have antithrombotic and anticoagulant activities in vivo. We studied the effects of bromelain on cell size distribution of isolated human platelets in vitro by Coulter Counter measurements. Preincubation of platelets with bromelain (10 micrograms/mL) completely prevented the thrombin (0.2 U/mL) induced platelet aggregation. Papain was less active in preventing platelet aggregation. In vitro, bromelain (0.1 microgram/mL) reduced the adhesion of bound, thrombin stimulated, fluorescent labeled platelets to bovine aorta endothelial cells. In addition, preincubation of platelets with bromelain, prior to thrombin, activation, reduced the platelet adhesion to the endothelial cells to the low binding value of unstimulated platelets. On the basis of mass concentrations, the proteases papain and trypsin were as effective as bromelain. Using a laser thrombosis model, the in vivo effects of orally and intraveneously applied bromelain on thrombus formation in rat mesenteric vessels were studied. Bromelain, orally applied at 60 mg/kg body weight, inhibited the thrombus formation in a time dependent manner, the maximum being after 2 hours in 11% of arterioles and 6% of venoles. Intravenous application at 30 mg/kg was slightly more active in reducing thrombus formation in arterioles (13%) and venoles (5%), suggesting that orally applied bromelain is biologically active. These results may help to explain some of the clinical effects observed after bromelain treatment in patients with thrombosis and related diseases.

  14. Evaluation of the splats properties and relation with droplets diameters in atomization process using a De Laval Nozzle

    Energy Technology Data Exchange (ETDEWEB)

    Planche, Marie-Pierre, E-mail: marie-pierre.planche@utbm.fr [LERMPS-UTBM Site de Sevenans, 90010 Belfort, Cedex (France); Khatim, Othmane; Dembinski, Lucas [LERMPS-UTBM Site de Sevenans, 90010 Belfort, Cedex (France); Bailly, Yannick [Institute FEMTO-ST/ENISYS, University of Franche-Comte, UMR CNRS 6174, 90000 Belfort (France); Coddet, Christian [LERMPS-UTBM Site de Sevenans, 90010 Belfort, Cedex (France)

    2013-01-15

    Properties of the powders developed during atomization process are essentially determined by process parameters. Experiments based on splat collection have been performed at different stages of the process evolution. Formation of splat formed as a result of impingement of the melted metallic droplets onto a substrate was studied. Splat characteristics have been determined from image analysis in function of process parameters and time progress. Droplet diameters were measured using laser Coulter analyzer. Direct relationships between splat diameter and droplet diameter were established. The atomization experiments point out the strong influence of two processing parameters (atomizing gas and melt nozzle diameter) on splat characteristics and particle size. It has been observed that increasing atomizing pressure leads to a decrease of mean splat diameter and width of the size distribution. Similar evolution was found for droplet diameter. Moreover, it is clearly shown that increasing melt nozzle diameter induces an increase of both splat diameters and droplet diameters. The results provide a better understanding of the influence of atomization parameters on the size distribution. Highlights: Black-Right-Pointing-Pointer In this study, changes of splat characteristics were examined versus operating parameters. Black-Right-Pointing-Pointer Splat diameter decreases with the increase of atomizing pressure. Black-Right-Pointing-Pointer Splat diameter decreases with the decrease of the melt nozzle diameter. Black-Right-Pointing-Pointer Changes of droplet diameter are in good agreement with those of splat diameter.

  15. Studies on Preparation of Dysprosium - 165 Metallic Macroaggregates for the Treatment of Rheumatoid Arthritis

    Energy Technology Data Exchange (ETDEWEB)

    Park, Kyung Bae; Kim, Jae Rok [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    1994-07-15

    Irradiation of 20 mg of natural Dy(NO{sub 3})3 in a neutron flux of 2 X10{sup 13} n/cm{sup 2} sec for 4 hours gave 5.76 Ci of {sup 165}Dy (specific activity, 610 mCi/mg Dy) with high radionuclidic purity (>99.9%). {sup 165}Dy-MA was prepared in a quantitative yield by reacting the aqueous solution of {sup 165}Dy(NO{sub 3})3 with sodium borohydride solution in 0.2N NaOH. Coulter particle analyzer exhibited mean particle size of 2.6 mum (range 1 approx 6 mum). Even though the {sup 165}Dy-MA suspension in saline was stored at 37 .deg. C for 24 hours of autoclaved at 121 .deg. C for 30 minutes, there was no significant change in particle size and leakage problem indicating the prepared {sup 165}Dy-MA is sufficiently stable. In-vivo retention studies were carried out by administering {sup 165}Dy-MA into the knee joint space of normal rabbits. Gamma camera analysis showed high retention in joint space of normal rabbits. Gamma camera analysis showed high retention in joining space even at 24 hours after administration (>99.9%) The ease with which the{sup 165}Dy-MA can be made in the narrow size range and their high in vitro and vivo stability make them attractive agents for radiation synovectomy.

  16. Evaluation of the accuracy of the diff3/50 with a cell by cell comparison.

    Science.gov (United States)

    Cembrowski, G S; Wilson, M K; Adelmeyer, S G; Chang, C K

    1983-09-01

    The traditional approach to the evaluation of accuracy of discrete differential counters uses a graphic comparison of automated and manual counts. This type of comparison introduces so much sampling variation that significant differences often are obscured. To exclude this sampling variability, one of three technologists, a reference technologist and the Coulter diff3/50, each performed a differential count on exactly the same 100 cells of 47 normal and 56 abnormal blood smears. Linear regression analysis demonstrated excellent agreement between the technologists for all cell types with the exception of band neutrophils. Comparison of the instrument results with the technologists' results showed very good agreement for segmented neutrophils, lymphocytes, monocytes, and eosinophils. The identification of band neutrophils, basophils, immature granulocytes and atypical lymphocytes requires improvement. The overall performance is acceptable, with only 11 leukocytes, on the average normal smear, misclassified out of 100 normal leukocytes (four bands called segs, four segs called bands, and three non-neutrophils incorrectly classified). We recommend similar evaluations for all discrete differential counters.

  17. Encapsulation of Platelet in Kefiran Polymer and Detection of Bioavailability of Immobilized Platelet in Probiotic Kefiran as A New Drug for Surface Bleeding

    Directory of Open Access Journals (Sweden)

    Anahita Jenab

    2015-11-01

    Full Text Available Background : Kefir contains lactic acid bacteria (Lactobacillus, Lactococcus, Leuconostoc, Acetobacter and Streptococcus and yeasts (Kluyveromyces, Torula, Candida, Saccharomyces .Kefiran is the polysaccharide produced by lactic acid bacteria in kefir.Methods : Kefiran was prepared from milk containing 0.5% fat and 10 grams kefir grains and was separated from kefir by ethanol (0.02 gram following entrapping the platelets to this polymer. Ligand of the platelet-polysaccharide was studied by FTIR.Results : FTIR results showed that the bands of C-O and C-O-C connections were formed and the polysaccharides had been attached to the receptors of the platelet glycoproteins (GP Ib,GPIIb / IIIa. Stability and encapsulation of the platelet and kefiran were assessed by Coulter Counter. Encapsulation of the platelets by polysaccharide at the beginning caused to reduce the number of platelets following by releasing of 50% of the platelets after 3 hours.Conclusion : The platelets were encapsulated in kefiran polymer and detected for bioavailability as new drug for surface bleeding. Also, kefiran has antimicrobial and antifungal properties. On the other hand, the existence of nisin in kefiran could be useful as an antibacterial lantibiotic. 

  18. Comparison of two flow-based imaging methods to measure individual red blood cell area and volume.

    Science.gov (United States)

    Tomaiuolo, Giovanna; Rossi, Domenico; Caserta, Sergio; Cesarelli, Mario; Guido, Stefano

    2012-12-01

    The red blood cells (RBCs) population is characterized by a high heterogeneity in membrane area, cellular volume, and mechanical properties, mainly due to the variety of mechanical and chemical stresses that a red cell undergoes in its entire life span. Here, we provide the first simultaneous area and volume measurements of RBCs flowing in microcapillaries, by using high-speed video microscopy imaging and quantitative data processing based on image analysis techniques. Both confined and unbounded flow conditions (depending on the relative size of RBCs and microcapillary diameter) are investigated. The results are compared with micropipette experiments from the literature and data from Coulter counter routine clinical blood tests. Good agreement is found for RBC volume, especially in the case of confined flow conditions. Surface area measurements, which are lacking in the routine clinical test, are of special interest being a potential diagnostic parameter of altered cell deformability and aggregability. Overall, our results provide a novel flow methodology suitable for high-throughput measurements of RBC geometrical parameters, allowing one to overcome the limits of classical static methods, such as micropipette aspiration, which are not suitable for handling a large number of cells.

  19. KCNK5 is Functionally Down-Regulated Upon Long-Term Hypotonicity in Ehrlich Ascites Tumor Cells

    Directory of Open Access Journals (Sweden)

    Signe Skyum Kirkegaard

    2013-11-01

    Full Text Available Background/Aims: Regulatory volume decrease (RVD in response to acute cell swelling is well described and KCNK5 (also known as TASK-2 or K2P5.1 has been shown to be the volume sensitive K+ channel in Ehrlich cells. Very little is, on the other hand, known about the effects of long-term hypotonicity on expression and function of KCNK5, thus we have investigated the effect of long-term hypotonicity (24h - 48h on KCNK5 in Ehrlich cells on the mRNA, protein and physiological levels. Methods: Physiological effects of long-term hypotonicity were measured using patch-clamp and Coulter counter techniques. Expression patterns of KCNK5 on mRNA and protein levels were established using real-time qPCR and western blotting respectively. Results: The maximum swelling-activated current through KCNK5 was significantly decreased upon 48h of hypotonicity and likewise the RVD response was significantly impaired after both 24 and 48h of hypotonic stimulation. No significant differences in the KCNK5 mRNA expression patterns between control and stimulated cells were observed, but a significant decrease in the KCNK5 protein level 48h after stimulation was found. Conclusion: The data suggest that the strong physiological impairment of KCNK5 in Ehrlich cells after long-term hypotonic stimulation is predominantly due to down-regulation of the KCNK5 protein synthesis.

  20. Single Nanoparticle Translocation Through Chemically Modified Solid Nanopore

    Science.gov (United States)

    Tan, Shengwei; Wang, Lei; Liu, Hang; Wu, Hongwen; Liu, Quanjun

    2016-02-01

    The nanopore sensor as a high-throughput and low-cost technology can detect single nanoparticle in solution. In the present study, the silicon nitride nanopores were fabricated by focused Ga ion beam (FIB), and the surface was functionalized with 3-aminopropyltriethoxysilane to change its surface charge density. The positively charged nanopore surface attracted negatively charged nanoparticles when they were in the vicinity of the nanopore. And, nanoparticle translocation speed was slowed down to obtain a clear and deterministic signal. Compared with previous studied small nanoparticles, the electrophoretic translocation of negatively charged polystyrene (PS) nanoparticles (diameter ~100 nm) was investigated in solution using the Coulter counter principle in which the time-dependent nanopore current was recorded as the nanoparticles were driven across the nanopore. A linear dependence was found between current drop and biased voltage. An exponentially decaying function ( t d ~ e -v/v0 ) was found between the duration time and biased voltage. The interaction between the amine-functionalized nanopore wall and PS microspheres was discussed while translating PS microspheres. We explored also translocations of PS microspheres through amine-functionalized solid-state nanopores by varying the solution pH (5.4, 7.0, and 10.0) with 0.02 M potassium chloride (KCl). Surface functionalization showed to provide a useful step to fine-tune the surface property, which can selectively transport molecules or particles. This approach is likely to be applied to gene sequencing.

  1. Comparison of three different methods of total carbon dioxide measurement.

    Science.gov (United States)

    Kilborn, Susan H.; Bonnett, Brenda N.; Pook, Harold A.

    1995-01-01

    The objective of this study was to compare total carbon dioxide (TCO(2)) levels measured by three different methods. Two hundred jugular venous blood samples from dogs admitted to the Veterinary Teaching Hospital, Ontario Veterinary College with various clinical disorders were analyzed by the Radiometer blood gas analyzer (BGA) and the Coulter DACOS analyzer. In 70 of these samples, TCO(2) was also measured by the Kodak Ektachem DTE chemistry analyzer. Comparison of the agreement between methods revealed intraclass correlation coefficients (ICC) of 0.63, 0.79, and 0.82 for the DACOS-Ektachem, DACOS-BGA and Ektachem-BGA comparisons, respectively. Evaluation of the effect of storage time on TCO(2) content of stored serum samples measured on the DACOS analyzer revealed a decrease of almost 2 mmol/L and a decrease in the intraclass correlation coefficient values between the DACOS analyzer and other methods after 7 hours storage time. The results of this observational study revealed lower agreement between the three methods in this study than previously reported between other methods of TCO(2) measurement. Possible reasons for the lower than expected agreement in this study included changes in DACOS values because of storage and differences in methodologies between the methods.

  2. Transcellular ion flow in Escherichia coli B and electrical sizing of bacterias.

    Science.gov (United States)

    Zimmermann, U; Schulz, J; Pilwat, G

    1973-10-01

    Dielectric breakdown of cell membranes and, in response, transcellular ion flows were measured in Escherichia coli B 163 and B 525 using a Coulter counter as the detector with a hydrodynamic jet focusing close to the orifice of the counter. Plotting the relative pulse height for compensated amplification of a certain size of the cells against increasing detector current, a rather sharp bend within the linear function was found, which did not occur when measuring fixed cells or polystyrene latex. The start current for transcellular ion flow causing the change of the slope is different for the potassium-deficient mutant B 525 in comparison with the wild-type B 163, indicating a change in the membrane structure of B 525 by mutation and demonstrating the sensitivity of the method for studying slight changes in membrane structure in general. The theoretical size distributions for two current values in the range of transcellular ion flow were constructed from the true size distribution at low detector currents, assuming an idealized sharp changeover of the bacterial conductivity from zero to one-third of the electrolyte conductivity.

  3. Flow cytometric evaluation of the intracellular bacterium, Wolbachia pipientis, in mosquito cells

    Science.gov (United States)

    Fallon, Ann M

    2014-01-01

    Wolbachia is an obligate intracellular bacterium (Anaplasmataceae, Rickettisales) that occurs in arthropods and filarial worms, and spreads by vertical transmission in the oocyte cytoplasm. In insects, reproductive distortions associated with Wolbachia, such as cytoplasmic incompatibility in mosquitoes, have potential value for controlling pests, including species that transmit human, animal and plant diseases. Wolbachia strains that propagate as a persistent infection in insect cell lines provide an important resource for developing the genetic tools that will facilitate these applications. Here I describe conditions for flow cytometric evaluation of Wolbachia growth in persistently infected mosquito cells. Cytometry parameters were established using uninfected mosquito cells and Escherichia coli as a surrogate for Wolbachia, and quantitation was correlated with cell counts determined with a Coulter electronic cell counter and bacterial counts based on optical density. The protocol was validated by showing depletion of Wolbachia in medium containing tetracycline and rifampicin, and sensitivity of Wolbachia to treatment of host cells with paraquat, an oxidizing agent, and lumiflavin, an inhibitor of riboflavin uptake. The Wolbachia peak on the flow cytometry histogram was shown to contain Wolbachia by DNA analysis using the polymerase chain reaction, and by infection of naive recipient cells. This approach will streamline investigation of Wolbachia growth in insect cell lines and facilitate identification of culture conditions that select for Wolbachia-infected cells. PMID:25300665

  4. An integrated ultrasonic-inductive pulse sensor for wear debris detection

    Science.gov (United States)

    Du, Li; Zhe, Jiang

    2013-02-01

    One approach to detect signs of potential machine failure is to detect wear debris in the lubrication oil of a rotating or reciprocating machine because the size and the concentration of wear debris particles in the oil show a direct relationship with the level of wear. In this article, a proof-of-principle integrated wear debris sensor consisting of an ultrasonic pulse sensor and an inductive pulse sensor for detecting wear debris in lubrication oil is presented. The ultrasonic pulse sensor detects all solid debris (metallic and non-metallic debris). A flow recess structure is utilized to ensure that all wear debris passes the acoustic focal region so that all debris can be accurately counted and sized. The inductive pulse sensor detects and counts all metallic debris (ferrous and non-ferrous) based on the inductive Coulter counting principle. By comparing the results from the two sensing components, the sensor is capable of differentiating and detecting non-metallic debris, ferrous metallic debris and non-ferrous metallic debris.

  5. Encapsulation of lycopene extract from tomato pulp waste with gelatin and poly(gamma-glutamic acid) as carrier.

    Science.gov (United States)

    Chiu, Y T; Chiu, C P; Chien, J T; Ho, G H; Yang, J; Chen, B H

    2007-06-27

    Tomato pulp waste, a byproduct obtained during the processing of tomato juice, has been shown to be a rich source of lycopene. The objectives of this study were to use gelatin and poly(gamma-glutamic acid) (gamma-PGA) as coating materials for the encapsulation of lycopene extract from tomato pulp waste. Initially, lycopene was extracted with supercritical carbon dioxide, followed by microencapsulation using an emulsion system consisting of 4.5% gelatin, 10% gamma-PGA, and 4.8% lycopene extract. Analysis of differential scanning calorimetry revealed that the thermal stability of the coating material could be up to 120 degrees C, with a mean particle size of 38.7 microm based on Coulter counter analysis. The total weight of microencapsulated powder was 617 microg with the yield of lycopene being 76.5%, indicating a 23.5% loss during freeze drying. During storage of microencapsulated powder, the concentrations of cis-, trans-, and total lycopene decreased along with increasing time and temperature. A fast release of lycopene in the powder occurred at pH 5.5 and 7.0, while no lycopene was released at pH 2.0 and 3.5.

  6. How does the greater white-toothed shrew, Crocidura russula, responds to long-term heavy metal contamination? - A case study

    Energy Technology Data Exchange (ETDEWEB)

    Marques, Carla Cristina [Centro de Biologia Ambiental, Departamento Biologia Animal, Faculdade de Ciencias, Universidade Lisboa, Campo Grande, 1749-016 Lisbon (Portugal)]. E-mail: ccmarques@fc.ul.pt; Sanchez-Chardi, Alejandro [Departament Biologia Animal (Vertebrats), Facultat de Biologia, Universitat Barcelona, Av. Diagonal 645, 08028 Barcelona (Spain); Gabriel, Sofia Isabel [Centro de Biologia Ambiental, Departamento Biologia Animal, Faculdade de Ciencias, Universidade Lisboa, Campo Grande, 1749-016 Lisbon (Portugal); Nadal, Jacint [Departament Biologia Animal (Vertebrats), Facultat de Biologia, Universitat Barcelona, Av. Diagonal 645, 08028 Barcelona (Spain); Viegas-Crespo, Ana Maria [Centro de Biologia Ambiental, Departamento Biologia Animal, Faculdade de Ciencias, Universidade Lisboa, Campo Grande, 1749-016 Lisbon (Portugal); Luz Mathias, Maria da [Centro de Biologia Ambiental, Departamento Biologia Animal, Faculdade de Ciencias, Universidade Lisboa, Campo Grande, 1749-016 Lisbon (Portugal)

    2007-04-15

    Heavy metals accumulation in parallel with the evaluation of physiological and biochemical effects resulting from continued metal exposure were considered here using for the first time the great white-toothed shrew Crocidura russula as an in vivo model. Shrews were originated from an abandoned lead/zinc mining area and from a reference area, both in Alentejo, southern Portugal. Hepatic contents of nickel, copper, zinc, cadmium, mercury and lead were quantified by Inductively Coupled Plasma Mass Spectrometer (ICP-MS). Haematological parameters (white blood cells, red blood cells, haemoglobin and haematocrit) were obtained in a Coulter Counter Analyser and biochemical markers of the redox balance (glutathione S-transferase, glutathione peroxidase, and glutathione reductase) activities were measured spectrophotometrically using a Duo-50 spectrophotometer. Compared with control animals, significantly higher concentration of hepatic cadmium (9.29 vs. 1.18 {mu}g/g dry weight) and nickel (1.56 vs. 0.343 {mu}g/g dry weight) were detected in the shrews collected in the mining area. However, no significant changes were observed on haematological or enzymatic parameters in animals exposed to metal pollution. The obtained results show that shrews are good bioaccumulators of toxic heavy metals, but very tolerant to their effects, revealing an interesting long-term adaptation to polluted environments. In addition, this study provides reference values for haematological parameters and antioxidant enzymes levels in C. russula, which may be relevant for comparative purposes in further studies.

  7. Characterization of winemaking yeast by cell number-size distribution analysis through flow field-flow fractionation with multi-wavelength turbidimetric detection.

    Science.gov (United States)

    Zattoni, Andrea; Melucci, Dora; Reschiglian, Pierluigi; Sanz, Ramsés; Puignou, Lluís; Galceran, Maria Teresa

    2004-10-29

    Yeasts are widely used in several areas of food industry, e.g. baking, beer brewing, and wine production. Interest in new analytical methods for quality control and characterization of yeast cells is thus increasing. The biophysical properties of yeast cells, among which cell size, are related to yeast cell capabilities to produce primary and secondary metabolites during the fermentation process. Biophysical properties of winemaking yeast strains can be screened by field-flow fractionation (FFF). In this work we present the use of flow FFF (FlFFF) with turbidimetric multi-wavelength detection for the number-size distribution analysis of different commercial winemaking yeast varieties. The use of a diode-array detector allows to apply to dispersed samples like yeast cells the recently developed method for number-size (or mass-size) analysis in flow-assisted separation techniques. Results for six commercial winemaking yeast strains are compared with data obtained by a standard method for cell sizing (Coulter counter). The method here proposed gives, at short analysis time, accurate information on the number of cells of a given size, and information on the total number of cells.

  8. 曲线拟合在血细胞分析中的应用%The Application of Curve Fitting among Analysis of Blood Cells

    Institute of Scientific and Technical Information of China (English)

    吴义满

    2011-01-01

    Objective The measurement error is fairly great in blood cell analyzer at present. To reduce the error, the curve fitting model based on Matlab is supposed to be applied. Methods The errors of the platelet count in measurement and Coulter theory were corrected with the method of curve fitting in this paper. Results The two examples with curve fitting method were completed and their results indicated that curve fitting based on Matlab was feasible and valid for correcting the errors from measurements. Conclusions Curve fitting based on Matlab is useful to correct the measurement error of blood cells.%目的 常见的血细胞分析仪测试误差比较大.为减小血细胞的测试误差,可以用Matlab进行曲线拟合的方法修正.方法 本文提出了用曲线拟合来修正血小板计数的测试误差和库尔特原理误差.结果 通过两个实例证明了曲线拟合修正血细胞的测试误差是可行和有效的.结论 利用Matlab曲线拟合修正血细胞的测试误差有一定实际意义.

  9. Significant role of adhesion properties of primary osteoblast-like cells in early adhesion events for chondroitin sulfate and dermatan sulfate surface molecules.

    Science.gov (United States)

    Stanford, C M; Solursh, M; Keller, J C

    1999-12-05

    The purpose of this study was to characterize the role of cell surface adhesive macromolecules through enzyme modulation and metabolic recovery prior to and during a kinetic cell adhesion assay. Primary rat calvarial osteoblast-like cells were derived from Sprague-Dawley calvarial plates. Cell adhesion kinetics was evaluated with the definition of first-order adhesion kinetics. Osteoblasts were incubated in an adhesion buffer for 1 h prior to a cell attachment assay using various enzymes to remove cell surface glycosaminoglycans (GAGs). A subtractive adhesion analysis was performed by plating cells at 5 x 10(4)/well for variable periods through 2 h. The medium was collected, the well surface washed and pooled, and the number of cells enumerated with a Coulter Counter. Cell adhesion demonstrated first-order logarithmic adhesion kinetics in the first 60 min. Scatchard analysis demonstrated a linear relationship. Preexposure of cells to various enzyme combinations demonstrated that 50% of the equilibrium adhesion was dependent on chondroitin sulfate or dermatan sulfate surface macromolecules. These results were confirmed with pretreatment with a metabolic inhibitor of GAG synthesis (beta-D-xyloside). These results suggest an important role for cell associated chondroitin sulfate and dermatan sulfate in cell adhesion in addition to Arg-Gly-Asp or integrin mediated adhesion events.

  10. Adenosine diphosphate-induced aggregation of human platelets in flow through tubes. I. Measurement of concentration and size of single platelets and aggregates.

    Science.gov (United States)

    Bell, D N; Spain, S; Goldsmith, H L

    1989-11-01

    A double infusion flow system and particle sizing technique were developed to study the effect of time and shear rate on adenosine diphosphate-induced platelet aggregation in Poiseuille flow. Citrated platelet-rich plasma, PRP, and 2 microM ADP were simultaneously infused into a 40-microliters cylindrical mixing chamber at a fixed flow ratio, PRP/ADP = 9:1. After rapid mixing by a rotating magnetic stirbar, the platelet suspension flowed through 1.19 or 0.76 mm i.d. polyethylene tubing for mean transit times, t, from 0.1 to 86 s, over a range of mean tube shear rate, G, from 41.9 to 1,000 s-1. Known volumes of suspension were collected into 0.5% buffered glutaraldehyde, and all particles in the volume range 1-10(5) microns 3 were counted and sized using a model ZM particle counter (Coulter Electronics Inc., Hialeah, FL) and a logarithmic amplifier. The decrease in the single platelet concentration served as an overall index of aggregation. The decrease in the total particle concentration was used to calculate the collision capture efficiency during the early stages of aggregation, and aggregate growth was followed by changes in the volume fraction of particles of successively increasing size. Preliminary results demonstrate that both collision efficiency and particle volume fraction reveal important aspects of the aggregation process not indicated by changes in the single platelet concentration alone.

  11. Using Failure Mode and Effect Analysis (FMEA for Performing Good Ploughing with Mouldboard

    Directory of Open Access Journals (Sweden)

    M Namdari

    2011-03-01

    Full Text Available Farm management needs creative methods to success. FMEA (Failure Modes and Effects Analysis is a new method to analyze potential reliability problems in the development cycle of the project, making it easier to take actions to overcome such issues, thus enhancing the reliability through design or process. Anticipating these failure modes, being the central step in the analysis, needs to be carried on extensively, in order to prepare a list of maximum potential failure modes. Risk is measured in terms of Risk Priority Number (RPN that is a product of occurrence, severity, and detection difficulty. This study attempted to improve clod mean weight diameter and soil inversion as indicators of tillage quality by FMEA methodology. The results showed that low soil moisture, slow speed of ploughing and great depth of ploughing is the most important factors that increase clod MWD with 900, 630 and 560 RPN, respectively. Also for soil inversion the slow speed of ploughing, not using coulter, low soil moisture and great depth of ploughing are important factors with 720, 648, 490 and 420 RPN. Using a split - split factorial experiment with 16 treatments and three replications also acknowledged the results of this method. After reforming the conditions and re-testing the experiment, results showed that clod MWD was reduced 20% and soil inversion increased 2% approximately. This study proposes the use of this technique in agricultural management.

  12. KCNK5 is Functionally Down-Regulated Upon Long-Term Hypotonicity in Ehrlich Ascites Tumor Cells

    DEFF Research Database (Denmark)

    Kirkegaard, S. S.; Wulff, Tune; Gammeltoft, S.;

    2013-01-01

    Background/Aims: Regulatory volume decrease (RVD) in response to acute cell swelling is well described and KCNK5 (also known as TASK-2 or K2P5.1) has been shown to be the volume sensitive K+ channel in Ehrlich cells. Very little is, on the other hand, known about the effects of long-term hypotoni......Background/Aims: Regulatory volume decrease (RVD) in response to acute cell swelling is well described and KCNK5 (also known as TASK-2 or K2P5.1) has been shown to be the volume sensitive K+ channel in Ehrlich cells. Very little is, on the other hand, known about the effects of long......-term hypotonicity on expression and function of KCNK5, thus we have investigated the effect of long-term hypotonicity (24h - 48h) on KCNK5 in Ehrlich cells on the mRNA, protein and physiological levels. Methods: Physiological effects of long-term hypotonicity were measured using patch-clamp and Coulter counter...... physiological impairment of KCNK5 in Ehrlich cells after long-term hypotonic stimulation is predominantly due to down-regulation of the KCNK5 protein synthesis.© 2013 S. Karger AG, Basel...

  13. Mineral Analysis of Pine Nuts (Pinus spp. Grown in New Zealand

    Directory of Open Access Journals (Sweden)

    Leo P. Vanhanen

    2013-04-01

    Full Text Available Mineral analysis of seven Pinus species grown in different regions of New Zealand; Armand pine (Pinus armandii Franch, Swiss stone pine (Pinus cembra L., Mexican pinyon (Pinus cembroides Zucc. var. bicolor Little, Coulter pine (Pinus coulteri D. Don, Johann’s pine (Pinus johannis M.F. Robert, Italian stone pine (Pinus pinea L. and Torrey pine (Pinus torreyana Parry ex Carrière, was carried out using an inductively coupled plasma optical emission spectrophotometer (ICP-OES analysis. Fourteen different minerals (Al, B, Ca, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, S and Zn were identified in all seven varieties, except that no Al or Na was found in Pinus coulteri D. Don. New Zealand grown pine nuts are a good source of Cu, Mg, Mn, P and Zn, meeting or exceeding the recommended RDI for these minerals (based on an intake of 50 g nuts/day while they supplied between 39%–89% of the New Zealand RDI for Fe. Compared to other commonly eaten tree-nuts New Zealand grown pine nuts are an excellent source of essential minerals.

  14. Identification and evaluation of biological control potential of B-401 endophytic bacteria in grasses on alpine grasslands%高寒草地禾草内生细菌B-401的鉴定及生物防治潜力评价

    Institute of Scientific and Technical Information of China (English)

    畅涛; 王涵琦; 杨成德; 王颖; 杨小利; 薛莉; 陈秀蓉; 徐长林

    2014-01-01

    To study the biological control potential of B-401 endophytic bacteria in grasses on alpine grasslands, the determination of bacteriostatic ability was carried out by dual culture against plant pathogenic fungi.B-401 inhibited the growth of Phomafoveata,Stysanus stemonitis,Colletotrichum coccodes,Fusarium avenaceum, Fusariumoxysporum,Alternaria solani,Bipolaris sorokiniana and Fusarium solani by 74.45%,71.57%, 70.05%,48.01%,56.01%,60.25%,64.65% and 45.01% respectively.The control efficacy of B-401 against potato gangrene was 52.67% when a 10 times dilution of fermentation broth was sprayed on potato tubers dur-ing the storage-period.The concentration of IAA secreted by B-401 in King’s medium containing 100 mg/L tryptophan was 3.42 mg/L,and the concentration in the medium without tryptophan was 2.60 mg/L.B-401 possessed the capacity for nitrogen fixation but it did not have the capacity for phosphate solubilization.Based on morphology and physiological and biochemical characters,combined with 16S rDNA gene sequence analysis, B-401 was indentified as Bacillus atrophaeus.%为了明确高寒草地禾草内生细菌B-401的生物防治潜力,采用平板对峙法测定了其抑菌能力。抑菌谱表明, B-401对马铃薯坏疽病菌、马铃薯褐腐病菌、马铃薯炭疽病菌、马铃薯枯萎病菌、马铃薯干腐病菌、番茄早疫病菌、小麦根腐病菌、孜然根腐病菌和黄瓜枯萎病菌的抑制率分别达74.45%,71.57%,70.05%,48.01%,56.01%,60.25%,64.65%,45.01%和31.79%。在贮藏库中进行10倍液喷雾,对马铃薯坏疽病的防效达52.67%。生物学功能测定表明,该菌株在含有色氨酸和不含色氨酸的 King培养基中分泌 IAA分别为3.42和2.60 mg/L,且具有固氮能力,无溶磷能力。对B-401通过形态特征和生理生化测定,结合16S rDNA序列同源性分析,鉴定其为萎缩芽孢杆菌(Bacillusatrophaeus)。

  15. Genetic toxicities of human teratogens.

    Science.gov (United States)

    Bishop, J B; Witt, K L; Sloane, R A

    1997-12-12

    Birth defects cause a myriad of societal problems and place tremendous anguish on the affected individual and his or her family. Current estimates categorize about 3% of all newborn infants as having some form of birth defect or congenital anomaly. As more precise means of detecting subtle anomalies become available this estimate, no doubt, will increase. Even though birth defects have been observed in newborns throughout history, our knowledge about the causes and mechanisms through which these defects are manifested is limited. For example, it has been estimated that around 20% of all birth defects are due to gene mutations, 5-10% to chromosomal abnormalities, and another 5-10% to exposure to a known teratogenic agent or maternal factor [D.A. Beckman, R.L. Brent, Mechanisms of teratogenesis. Ann. Rev. Pharmacol. Toxicol. 24 (1984) 483-500; K. Nelson, L.B. Holmes Malformations due to presumed spontaneous mutations in newborn infants, N. Engl. J. Med. 320 (1989) 19-23.]. Together, these percentages account for only 30-40%, leaving the etiology of more than half of all human birth defects unexplained. It has been speculated that environmental factors account for no more than one-tenth of all congenital anomalies [D.A. Beckman, R.L. Brent, Mechanisms of teratogenesis, Ann. Rev. Pharmacol. Toxicol. 24 (1984) 483-500]. Furthermore, since there is no evidence in humans that the exposure of an individual to any mutagen measurably increases the risk of congenital anomalies in his or her offspring' [J.F. Crow, C. Denniston, Mutation in human populations, Adv. Human Genet. 14 (1985) 59-121; J.M. Friedman, J.E. Polifka, Teratogenic Effects of Drugs: A Resource for Clinicians (TERIS). The John Hopkins University Press, Baltimore, 1994], the mutagenic activity of environmental agents and drugs as a factor in teratogenesis has been given very little attention. Epigenetic activity has also been given only limited consideration as a mechanism for teratogenesis. As new molecular

  16. 免疫固定电泳对多发性骨髓瘤的临床应用价值%Clinical Value of Immunofixation Electrophoresis on The Diagnosis of Multiple Myeloma

    Institute of Scientific and Technical Information of China (English)

    姚红; 吴亚楠

    2014-01-01

    Objective To study the clinical value immunofixation electrophoresis on the diagnosis of multiple myeloma ( MM) .Methods Serum protein electrophoresis and immunofixation electrophoresis of 32 patients diagnosed with multiple mye-loma was measured by France Sebia HYDRASYS automatic electrophoresis .2States Beckman Immage 800 specific protein analy-zer was used to detect serum immunoglobulins ,light chain determination of urine and blood .Bence-Jones protein of urine was de-tected by handmade nitric acid method .Results There were 14 IgG (43.75%),9 IgA (28.13%),1 IgM (3.13%),and 2 light chain (6.25%).There were 13 Kappa chain (40.63%),11 lambda chain (34.38%) and 18 Bence-Jones protein positive patients (56.25%).2Immunofixation electrophoresis showed that M protein positive rate was 96.9%.Conclusion IgG、IgA ac-count for the largest proportion in the immunoglobulin types ,kappa chain is slightly more than lambda chain ,and the positive rate of Bence-Jones protein of urine is higher than the negative rate .Immunofixation electrophoresis has important clinical value on the diagnosis and classification of MM .%目的:探讨免疫固定电泳对多发性骨髓瘤( MM)的临床诊断价值。方法应用法国Sebia HYDRASYS全自动电泳仪,对32例MM患者进行血清蛋白电泳测定和免疫固定电泳测定。应用美国Beckman Immage 800特定蛋白分析仪进行血清免疫球蛋白和血、尿轻链测定。采用手工硝酸法进行尿本周氏蛋白测定。结果32例多发性骨髓瘤中IgG型14例(43.75%),IgA型9例(28.13%),IgM型1例(3.13%),轻链型2例(6.25%)。所有类型中κ链13例(40.63%),λ链11例(34.38%),尿本周氏蛋白阳性18例(56.25%)。免疫固定电泳检测M蛋白阳性率为96.9%。结论 MM患者以IgG型、IgA型比例最高,轻链类型中κ链比例稍高于λ链,尿本周氏蛋白阳性者多于尿本周氏蛋白阴性患者,免疫固定电

  17. Scientific Analysis Cover Sheet for Radionuclide Screening

    Energy Technology Data Exchange (ETDEWEB)

    G. Ragan

    2002-08-09

    pressurized water reactor (PWR) fuel, U.S. Department of Energy (DOE) spent nuclear fuel (DSNF), and high-level waste (HLW). Average and outlying (high burnup, high initial enrichment, low age, or otherwise exceptional) forms of each waste-form type are considered. This analysis has been prepared in accordance with a technical work plan (BSC 2002c). In a review of Revision 00 of this radionuclide screening analysis, the NRC found that ''processes that affect transport in the biosphere, such as uptake by plants and bioaccumulation are not accounted for'' and that ''the direct exposure pathway is not accounted for'' (Beckman 2001, Section 5.3.2.1). The NRC also found that the solubility and sorption classes were too broadly defined, noting, for example, that Se is in the same solubility and sorptivity groups as Np and U, yet is ''more soluble than Np and U by several orders of magnitude'' (Beckman 2001, Section 5.3.2.1). This revision seeks to build upon the strengths of the earlier screening method while responding to the specific concerns raised by the NRC and other reviewers. In place of simple inhalation and ingestion dose conversion factors, the revised radionuclide screening uses screening factors that also take into account soil accumulation, uptake by plants, exposure to contaminated ground, and other features of the biosphere that were neglected in the previous screening. Whereas the previous screening analysis allowed only two solubility classes (soluble and insoluble), the revised screening introduces an intermediate solubility class to better segregate the radionuclides into transport groups.

  18. 联合检测血清HbAlc和Cys-C对1型糖尿病肾病早期诊断的研究%The Study on Joint Detection Serum HbAlcand Cys-C for Early Diagnosis of 1 Type Diabetic Nephropathy

    Institute of Scientific and Technical Information of China (English)

    崔靖

    2015-01-01

    Objective Through the 1 diabetes nephropathy patients with different disease period of serum glycated hemoglobin andcystatin C joint detection, evaluation of the combination 1 diabetes nephropathy early diagnosis value.Methods Selected 1 diagnosed diabetes nephropathy patients in our hospitalandsame period the healthy check-up patients, each group had 100 cases, adopt BECKMAN CX 4, automatic biochemical analyzer and testing sample serum glycated hemoglobin and cystatin C. Results1 diabetes nephropathy patients inmeasurement of serum glycated hemoglobin and bladder or element-C concentration level was higher thanthe controlgroup,P<0.05, had difference statistically significance, 1 diabetes nephropathy patients different disease period the serum glycatedhemoglobin and cystatin C concentration level wasincreasing resistance increases, a positive correlation between trend ,P<0.05, had difference statistically signiifcance.Conclusion Serum glycated hemoglobin and cystatin C determination helps to 1type diabetic nephropathy early diagnosis, clinical doctors can provide reliable accurate valuable diagnostic basis for guidance.%目的:检测1型糖尿病肾病不同病期患者的血清中糖化血红蛋白和胱抑素-C水平,评价其组合对1型糖尿病肾病早期诊断的价值。方法选择本院确诊为1型糖尿病肾病患者和同期健康体检者,各100例,采用BECKMAN CX4全自动生化分析仪同时检测样本血清中糖化血红蛋白和胱抑素-C的含量。结果1型糖尿病肾病患者检测血清中糖化血红蛋白和胱抑素-C浓度水平高于对照组,P<0.05,差异具有统计学意义。1型糖尿病肾病患者不同病期的血清中糖化血红蛋白和胱抑素-C浓度水平呈递增性升高,趋势呈正相关,各期与1期比较,P<0.05,差异具有统计学意义。结论血清中糖化血红蛋白和胱抑素-C测定有助于1型糖尿病肾病早期诊断,能为临床医生提供可靠准确有价值的诊断指导依据。

  19. Background: apolipoprotein-a1 (apoa1 has been proposed as indicator of nutritional state, but controversy exists on the matter. Apolipoproteína A1 para evaluar el estado nutricional en niños con y sin malaria

    Directory of Open Access Journals (Sweden)

    Silvia Blair Trujillo

    2007-04-01

    Full Text Available Objective: to compare serum apoA1 with anthropometric evaluation as a diagnostic test to of nutritional state in children with or without malaria. Material and methods: descriptive, prospective and cross-sectional design, with blind and simultaneous use of diagnostic tests. Serum ApoA1 was measured with a nephelometric method (APOA, Beckman® Laboratory. Results: 262 children were evaluated. The frequency of some anthropometric risk of malnutrition was 72% in malaric children, and 70% in the non-malaric ones. Two out of every three children had less than 0,94 g/L (lowest normal limit of apoA1. There was no significant correlation between apoA1 and each one of the anthropometric indicators, and the Spearman's coefficients were lower than 0,1 in every instance. Sensitivity and specificity of apoA1 were, respectively, 31% and 83% among non-malaric children, as compare with the anthropometrical nutritional classification; the corresponding figures among malaric children were 83% and 27%, respectively. Conclusion: apoA1 is not a useful tool for diagnosis of the nutritional state, either in malaric or nonmalaric children. Antecedentes: la apolipoproteína A1 (apoA1 se ha propuesto como indicador del estado nutricional, pero existe controversia al respecto. Objetivo: comparar la apoA1 sérica con la evaluación antropométrica como prueba diagnóstica del estado nutricional de niños (4-11 años con malaria y sin ella. Metodología: diseño descriptivo, prospectivo y transversal, con uso ciego y simultáneo de las dos pruebas diagnósticas. ApoA1 sérica se midió con método nefelométrico (APOA, Laboratorio Beckman®. Resultados: fueron evaluados 262 niños. La frecuencia de algún riesgo antropométrico de desnutrición fue 72% en niños maláricos y 70% en niños sin malaria. Dos de cada tres niños presentaron menos de 0,94 g/L (límite inferior normal de apoA1. No hubo correlación significativa entre apoA1 y cada indicador antropométrico y los

  20. Preparo de hidrolisados protéicos para a análise de aminoácidos Preparation of protein hydrolysates for amino acid analysis

    Directory of Open Access Journals (Sweden)

    Carlos Roberto Bernardi

    2003-12-01

    Full Text Available Amostras de milho, farelo de soja e hidrolisado ácido de caseína foram submetidas à hidrólise ácida em estufa (110 ± 5°C, durante 22horas com ácido clorídrico 6 N, em ampolas de vidro seladas sob vácuo. Os hidrolisados foram preparados por quatro métodos: 1 evaporação em evaporador rotativo sob vácuo; 2 evaporação em câmaras a vácuo em presença de pastilhas de NaOH; 3 evaporação em bloco de aquecimento sob fluxo de argônio; 4 neutralização com solução padrão de citrato/NaOH. Os aminoácidos foram separados e quantificados em analisador específico Beckman System 6300 com derivatização pós-coluna por ninidrina. O método de neutralização dos reagentes da hidrólise apresentou os melhores resultados considerando todos os aminoácidos. Além disso, foi mais rápido e prático no preparo de um grande número de amostras. Quanto aos métodos de evaporação, o uso de evaporador rotativo apresentou perdas significativas apenas para treonina e serina na amostra farelo de soja; sob fluxo de argônio observou-se baixos teores para treonina, fenilalanina e lisina; em câmara sob vácuo verificou-se perdas significativas para treonina, serina e tirosina.Samples of corn, soybean corn and casein (acid hydrolysate were submitted to acid hydrolysis in oven (110 ± 5°C, for 22 hours with 6 N HCl in sealed ampoules under vacuum. Four methods of hydrolysate preparation were used: 1 evaporation to dryness in rotary evaporator under vacuum, 2 evacuated desiccator under vacuum and NaOH, 3 flushing the open vial with argon and 4 neutralisation with standard citrate/NaOH solution. Amino acids were isolated and quantified in specific analyzer Beckman System 6300 with ninhidrin post-column derivatization. The neutralization method presented the best result considering all analysed amino acids. Besides, it was more practical for rapid processing of a large number of samples. With regard to the evaporation methods in the rotary evaporator

  1. The Nucleosides Contents and Their Variation in Natural Cordyceps sinensis and Cultured Cordyceps Mycelia%天然与发酵培养冬虫夏草中核苷类成分的含量及其变化

    Institute of Scientific and Technical Information of China (English)

    李绍平; 李萍; 季晖; 朱荃; 董婷霞; 詹华强

    2001-01-01

    Aim: To compare the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia, and to study the effect of humidity and heat on the content of nucleosides. Methods: The contents of nucleosides were determined by using high performance capillary electrophoresis (HPCE). Beckman P/ACE System 5010 apparatus equipped with a UV detector and a Beckman untreated fused-silica capillary (57 cm ×75 μm, 50 cm effective length) was used. Before sample injection, the capillary was rinsed with 1 mol.L-1 sodium hydroxide solution and running buffer for 5 min, respectively. A voltage of 20 kV was applied for the separation.Pressure injection was 586 kPa for 6 seconds, and the wavelength of detector was 254 nm. The running time was 20 min at 20 ℃. The effect of humidity and heat on the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia was observed for 1, 3, 5 and 10 days at temperature 40 ℃, and relative humidity 75%. Results: The content of nucleosides from natural Cordyceps sinensis was higher than that from cultured Cordyceps mycelia. But the contents of nucleosides from freshly collected natural Cordyceps sinensis were very low, even below the limit of quantitation. The contents of nucleosides from natural Cordyceps sinensis were significantly increased by humidity and heat, but this phenomenon was not observed in cultured Cordyceps mycelia. Conclusion:There are differences between the nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia. The nucleosides in natural Cordyceps sinensis may be derived from the degradation of nucleic acids. This implies that adenosine being used for the quality control of natural Cordyceps sinensis may have to be reconsidered.%目的比较天然和人工冬虫夏草中核苷类成分的含量,并对其变化进行考察.方法采用高效毛细管电泳法测定天然和人工冬虫夏草中核苷类成分的含量;考察湿热对冬虫

  2. Clinical significance of determination of serum cystatin C, lipoprotein (α) and high sensitivity C-reactive protein in type 2 diabetic patients with early renal impairment%糖尿病肾病患者血清半胱氨酸蛋白酶抑制剂C、脂蛋白(α)和超敏C反应蛋白的检测及其临床意义

    Institute of Scientific and Technical Information of China (English)

    黄勇武; 刘镜光; 卢卫国

    2010-01-01

    目的 检测2型糖尿病患者血清中半胱氨酸蛋白酶抑制剂C(CysC)、脂蛋白(α)[LP(α)]和超敏C反应蛋白(hs-CRP)水平,并探讨其临床意义.方法 126例2型糖尿病患者分为单纯糖尿病组(DM组)40例、早期糖尿病肾病组(EDN组)44例、临床糖尿病肾病组(DN组)42例,以70例健康体检者作为对照组,采用BECKMAN UniCel Dxc600全自动生化分析仪分别检测血清中的CysC、LP(α)以及hs-CRP水平.结果 2型糖尿病患者中CysC、LP(a)、hs-CRP水平均高于正常对照组(P<0.01),临床糖尿病肾病组患者的CysC、LP(a)、hs-CRP水平也明显高于单纯糖尿病组和早期糖尿病肾病组(P<0.01),在2型糖尿病患者中,血清中的CysC、LP(α)、hs-CRP与尿微量白蛋白(mALB)呈正相关,联合检测CysC、LP(α)、hs-CRP对糖尿病肾病的阳性率达88.5%.结论 CysC、LP(α)、hs-CRP作为诊断糖尿病早期肾损害的敏感指标,联合检测更具实用价值.

  3. Annual symposium on Frontiers in Science

    Energy Technology Data Exchange (ETDEWEB)

    Metzger, N.; Fulton, K.R.

    1998-12-31

    This final report summarizes activities conducted for the National Academy of Sciences' Annual Symposium on Frontiers of Science with support from the US Department of Energy for the period July 1, 1993 through May 31, 1998. During the report period, five Frontiers of Science symposia were held at the Arnold and Mabel Beckman Center of the National Academies of Sciences and Engineering. For each Symposium, an organizing committee appointed by the NAS President selected and planned the eight sessions for the Symposium and identified general participants for invitation by the NAS President. These Symposia accomplished their goal of bringing together outstanding younger (age 45 or less) scientists to hear presentations in disciplines outside their own and to discuss exciting advances and opportunities in their fields in a format that encourages, and allows adequate time for, informal one-on-one discussions among participants. Of the 458 younger scientists who participated, over a quarter (124) were women. Participant lists for all symposia (1993--1997) are attached. The scientific participants were leaders in basic research from academic, industrial, and federal laboratories in such disciplines as astronomy, astrophysics, atmospheric science, biochemistry, cell biology, chemistry, computer science, earth sciences, engineering, genetics, material sciences, mathematics, microbiology, neuroscience, physics, and physiology. For each symposia, the 24 speakers and discussants on the program were urged to focus their presentations on current cutting-edge research in their field for a scientifically sophisticated but non-specialist audience, and to provide a sense of the experimental data--what is actually measured and seen in the various fields. They were also asked to address questions such as: What are the major research problems and unique tools in their field? What are the current limitations on advances as well as the frontiers? Speakers were asked to provide a

  4. Assessing composition and structure of soft biphasic media from Kelvin–Voigt fractional derivative model parameters

    Science.gov (United States)

    Zhang, Hongmei; Wang, Yue; Fatemi, Mostafa; Insana, Michael F.

    2017-03-01

    Kelvin–Voigt fractional derivative (KVFD) model parameters have been used to describe viscoelastic properties of soft tissues. However, translating model parameters into a concise set of intrinsic mechanical properties related to tissue composition and structure remains challenging. This paper begins by exploring these relationships using a biphasic emulsion materials with known composition. Mechanical properties are measured by analyzing data from two indentation techniques—ramp-stress relaxation and load-unload hysteresis tests. Material composition is predictably correlated with viscoelastic model parameters. Model parameters estimated from the tests reveal that elastic modulus E 0 closely approximates the shear modulus for pure gelatin. Fractional-order parameter α and time constant τ vary monotonically with the volume fraction of the material’s fluid component. α characterizes medium fluidity and the rate of energy dissipation, and τ is a viscous time constant. Numerical simulations suggest that the viscous coefficient η is proportional to the energy lost during quasi-static force-displacement cycles, E A . The slope of E A versus η is determined by α and the applied indentation ramp time T r. Experimental measurements from phantom and ex vivo liver data show close agreement with theoretical predictions of the η -{{E}A} relation. The relative error is less than 20% for emulsions 22% for liver. We find that KVFD model parameters form a concise features space for biphasic medium characterization that described time-varying mechanical properties. The experimental work was carried out at the Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Methodological development, including numerical simulation and all data analysis, were carried out at the school of Life Science and Technology, Xi’an JiaoTong University, 710049, China.

  5. Translation, modification and validation of the Chinese version of a knowledge assessment instrument regarding pressure ulcer prevention

    Institute of Scientific and Technical Information of China (English)

    Ming Liu; Hao-Bin Yuan; Wei-Ju Chen; Chiuyin Poon; Meihua Hsu; Bo Zhang

    2016-01-01

    Objective: This study sought to translate, modify and validate an instrument developed by Beckman and colleagues to assess the knowledge of clinical nurses regarding pressure ulcers. Methods: A methodological study design was used. The instrument was translated into Chinese and back-translated into English. A six-expert panel was invited to evaluate the content validity, and a pilot test was subsequently performed on the test-retest stability of the translated instrument. A convenience sampling method was used to recruit 240 nurses from a university teaching hospital in the Mainland of China. In total, 186 valid questionnaires were collected with a 77.5%valid return rate. The validity of the multiple-choice test items (item difficulties and discriminating indices) and internal consistency reli-ability were evaluated. Results: The translated and modified instrument demonstrated acceptable psychometric properties, as follows: (1) the overall content validity index (CVI) was 0.91, (2) the overall test-retest reliability was 0.826, (3) the item difficulty indices were between 0.46 and 0.93, (4) the overall values for discrimination were 0.28e0.55, and (5) the Cronbach's a for the internal consistency were 0.792 for the overall in-strument and 0.426e0.804 for the sub-themes. Conclusions: This study represents the first trial to translate and modify an existing instrument that measures the knowledge of pressure ulcers in a Chinese Mainland sample. The instrument demonstrated acceptable psychometric properties and could be applied in cross-cultural nursing practices, including nursing education, research and practice, to evaluate knowledge about pressure ulcer prevention.

  6. [Effects of ruta SSP on the activity of the smooth gastrointestinal muscle isolated of rat].

    Science.gov (United States)

    Grigorjev, Carlota A; Brizuela, Nilda Y

    2010-01-01

    Ruta graveolens L. and Ruta chalepensis L. are plants used in folkloric medicine as antispasmodics, digestive and for intestinal gases. Animals used as experimental model were rats of the Wistar line, adult females, clinically healthy and with a weight average of 250 grams. We used strips of stomach and duodenum. Each one of the segments mounted on two stirrups in a bath of organ isolated with Ringer-lactate solution, at 37° C , pH: 7.3-7.4, and bubbled with 95% O2, 5% CO2. One of the stirrups was connected vertically to the bottom of the bath and the other to a transducer of tension connected to a Beckman polygraph. We applied 500 mgs of basal tension. After the stabilization, the ethanolic extract of Ruta ssp was added in increasing doses. At 50μl/ml the tone lower 23% in small intestine and lower 27% in stomach. However at 100 μl/ml the tone lower 32% and 35% respectively. In the other parameters the amplitude decrease 50% in the stomach at dose of 5 μl/ml while in the small intestine the amplitude lower 60%. With 10 μl/ml the amplitude change in both organs ( 96% in small intestine, and 75% in stomach). The frequency changes in both organs ( 32% in small intestine, and 50% in stomach) at 10 μl/ml Rue showed decreased effects on isolated small intestine and stomach were is dose dependent, maybe we were demonstrated the effects digestive of Ruta.

  7. Correlation of fine needle aspiration cytology findings with thyroid function test in cases of lymphocytic thyroiditis.

    Science.gov (United States)

    Sood, Neelam; Nigam, Jitendra Singh

    2014-01-01

    Background. Chronic lymphocytic thyroiditis is the second most common thyroid lesion diagnosed on FNAC after goiter. FNAC is reliable tool in the diagnosis of thyroid lesion. Objective. To correlate FNAC cytologic findings with TFT in the lymphocytic thyroiditis. Methods. 175 patients with thyroid swellings were referred for FNAC as well as TFT during 2011-2013. Fine needle aspiration cytology was performed using non-aspiration or aspiration techniques and TFT performed on Beckman culter access 2. Results. Lymphoid infiltrate was seen in 55 cases. The commonest age group of lymphocytic thyroiditis was 21-30 years with male : female ratio being 1 : 10. Anti-TPO and TSH were elevated in 96.16% (25/26) of cases with grade 3 lymphoid infiltrate, 94.12% (16/17) of cases with grade 2, and 91.67% (11/12) of cases with 1 grade. Increased anti-TPO with raised TSH without any lymphoid infiltrate was seen in 5 cases and 5 cases showed only raised TSH without raised anti-TPO and without any lymphoid infiltrate. We observed that grade 3 lymphocytic infiltration has correlation with anti-TPO and TSH together or TSH alone but not with anti-TPO alone. We also observed that anti-TPO and TSH together are significant even if no lymphocytic infiltration is present. Conclusion. Grade 3 lymphocytic infiltration has statistical correlation with anti-TPO and TSH together or TSH alone but not with anti-TPO alone. Anti TPO was adjunct to TSH in grade 3. The presence of Hurthle cell change, giant cells, and granulomas has no statistical correlation with lymphocytic thyroiditis.

  8. Correlation of Fine Needle Aspiration Cytology Findings with Thyroid Function Test in Cases of Lymphocytic Thyroiditis

    Directory of Open Access Journals (Sweden)

    Neelam Sood

    2014-01-01

    Full Text Available Background. Chronic lymphocytic thyroiditis is the second most common thyroid lesion diagnosed on FNAC after goiter. FNAC is reliable tool in the diagnosis of thyroid lesion. Objective. To correlate FNAC cytologic findings with TFT in the lymphocytic thyroiditis. Methods. 175 patients with thyroid swellings were referred for FNAC as well as TFT during 2011–2013. Fine needle aspiration cytology was performed using non-aspiration or aspiration techniques and TFT performed on Beckman culter access 2. Results. Lymphoid infiltrate was seen in 55 cases. The commonest age group of lymphocytic thyroiditis was 21–30 years with male : female ratio being 1 : 10. Anti-TPO and TSH were elevated in 96.16% (25/26 of cases with grade 3 lymphoid infiltrate, 94.12% (16/17 of cases with grade 2, and 91.67% (11/12 of cases with 1 grade. Increased anti-TPO with raised TSH without any lymphoid infiltrate was seen in 5 cases and 5 cases showed only raised TSH without raised anti-TPO and without any lymphoid infiltrate. We observed that grade 3 lymphocytic infiltration has correlation with anti-TPO and TSH together or TSH alone but not with anti-TPO alone. We also observed that anti-TPO and TSH together are significant even if no lymphocytic infiltration is present. Conclusion. Grade 3 lymphocytic infiltration has statistical correlation with anti-TPO and TSH together or TSH alone but not with anti-TPO alone. Anti TPO was adjunct to TSH in grade 3. The presence of Hurthle cell change, giant cells, and granulomas has no statistical correlation with lymphocytic thyroiditis.

  9. A Centrifuge-Based Technique for Dry Extraction of Air for Ice Core Studies of Carbon Dioxide.

    Science.gov (United States)

    Grachev, A. M.; Brook, E. J.

    2008-12-01

    High resolution CO2 data from the Law Dome ice core document an abrupt ~10 ppm drop in CO2 at about 1600 AD (MacFarling Meure et al., Geophys. Res Lett., v. 33, L14810), which has been attributed to changes in human activities. CO2 measurements in ice cores are difficult, however, making verification of this feature an important task. We are undertaking a high-resolution study of CO2 between 1400 and 1800 AD in the WAIS Divide (Antarctica) ice core with a new dry extraction technique. The need for a dry extraction technique as opposed to a melt-refreeze technique in studies of CO2 from ice cores arises because of the well-documented artifacts in CO2 imposed by the presence of liquid water. Three dry-extraction methods have been employed by previous workers to measure CO2: needle-crushing method, ball-bearings method, and cheese-grater method (B. Stauffer, in: Encyclopedia of Quaternary Science, p. 1181, Elsevier 2007). Each has limitations, and we propose a simpler dry extraction technique, based on a large-capacity refrigerated centrifuge (the "centrifuge technique"), which eliminates the need to employ cryogenic temperatures to collect extracted gas and is more compatible with high sample throughput. The technique is now being tested on ~25-gram WAIS Divide samples in conjunction with CO2 measurements with a gas chromatograph. The technique employs a Beckman J- 6B centrifuge, in which evacuated stainless steel flask is placed: the flask has a weight inside positioned directly over a tall-standing piece of ice whose cross-section is small compared to that of the flask. Upon acceleration to 3000 rpm the weight moves down and presses the ice sample into a thin tablet covering flask's bottom, yielding the air extraction efficiency of ~80%. Preliminary tests suggest that precision and accuracy can be achieved at the level of ~1 ppm once the system is fine-tuned.

  10. Characterization of Fricke dosimeter developed at Centro Regional de Ciencias Nucleares, Recife, PE, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Vivianne L.B.; Cunha, Manuela S.; Santos, Carla D.A.; Figueiredo, Marcela D.C.; Rodrigues, Kelia R.G.; Melo, Roberto T.; Hazin, Clovis A., E-mail: vlsouza@cnen.gov.b, E-mail: manusc06@hotmail.co, E-mail: daysebutterfly@hotmail.co, E-mail: keliareja@hotmail.co, E-mail: rtmelo@cnen.gov.b, E-mail: chazin@cnen.gov.b [Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil)

    2009-07-01

    Fricke Xylenol Gel (FXG) is a modified Fricke dosimeter system that was tested at the Centro Regional de Ciencias Nucleares (CRCN/CNEN), in Recife, Brazil. The Fricke dosimeter system has been used in our laboratory successfully. We decided to test the FXG in our laboratory because this dosimetric system presents some advantages in relation to the Fricke dosimeter, such as: easy preparation, more sensitivity for doses between 5 - 30 Gy, good reproducibility, tissue equivalence and multidimensional dose information. The dosimetric solution was synthesized with 0.392 g of ferrous ammonium sulphate, 0.0060 g of sodium chloride, 0.0076 g of xylenol orange, 5.0 g of gelatine (270 bloom porcine) and 300 mul of sulphuric acid in 100 ml of water. First, the water-gelatin mixture was heated and continuously stirred with a magnetic stirrer while the others reagents were added and the temperature was raised to 45 deg C. Once the solution ready it was transferred to acrylic cuvettes and kept in dark under refrigeration at 5 deg C during 12 h. Characteristics such as absorption spectrum, stability and linearity were evaluated by spectrophotometry (Beckman DU-640 UV-visible spectrophotometer). The developed material presented a good performance: ferric ion concentration at 589 nm, after irradiation; linear dose-response curve between 5 and 30 Gy; and saturation region for doses higher than 30 Gy. One disadvantage of the FXG system is its poor storage stability, because the non-irradiated samples suffer a selfdegradation and this could be a limitation for the use of FXG system. (author)

  11. Comparison between a second generation automated multicapillary electrophoresis system with an automated agarose gel electrophoresis system for the detection of M-components.

    Science.gov (United States)

    Larsson, Anders; Hansson, Lars-Olof

    2008-01-01

    During the last decade, capillary electrophoresis (CE) has emerged as an interesting alternative to traditional analysis of serum, plasma and urine proteins by agarose gel electrophoresis. Initially there was a considerable difference in resolution between the two methods but the quality of CE has improved significantly. We thus wanted to evaluate a second generation of automated multicapillary instruments (Capillarys, Sebia, Paris, France) and the high resolution (HR) buffer for serum or plasma protein analysis with an automated agarose gel electrophoresis system for the detection of M-components. The comparison between the two systems was performed with patients samples with and without M-components. The comparison included 76 serum samples with M-components > 1 g/L. There was a total agreement between the two methods for detection of these M-components. When studying samples containing oligoclonal bands/small M-components, there were differences between the two systems. The capillary electrophoresis system detected a slightly higher number of samples with oligoclonal bands but the two systems found oligoclonal bands in different samples. When looking at resolution, the agarose gel electrophoresis system yielded a slightly better resolution in the alpha and beta regions, but it required an experienced interpreter to be able to benefit from the increased resolution. The capillary electrophoresis has shorter turn-around times and bar-code reader that allows positive sample identification. The Capillarys in combination with HR buffer gives better resolution of the alpha and beta regions than the same instrument with the beta1-beta2+ buffer or the Paragon CZE2000 (Beckman) which was the first generation of capillary electrophoresis systems.

  12. DXI800型化学发光仪定量检测甲胎蛋白初步性能评定

    Institute of Scientific and Technical Information of China (English)

    梁鑫; 杨琳; 吴玲

    2011-01-01

    目的 按照(WS/T 228-2002)中的的要求,对Beckman Couter Uncel DXI 800型化学发光仪的甲胎蛋白(AFP)项目进行初步评定,了解DXI 800型化学发光仪在AFP项目上的线性、偏倚、线性漂移、样品交叉污染和精密度等性能.方法 选用低、中、高3种水平的校准品每天按照中、高、底、中、中、低、低、高、高、中的顺序连续检测5 d(若有样品被拒绝则按照这个顺序继续做),得到结果 后再分析.结果 低值偏倚为1.63,中值的偏倚为6.55,高值的偏倚为3.81,均小于其对应的允许偏倚.低值的不精密度为5.90,中值的不精密度为5.58,高值的不精密度为3.62,均小于其允许不精密度.结论 DXI 800型化学发光仪在定量检测AFP这个项目上的偏倚及不精密度上的性能都是可接受的.

  13. Optimización de la extracción de ADN de Passiflora ligularis para el análisis por medio de marcadores moleculares

    Directory of Open Access Journals (Sweden)

    Gina Solano-Flórez

    2009-04-01

    Full Text Available Extraction of high quality DNA of Passiflora ligularis for its analysis with molecular markers. Objective. To standardize a precise andefficient DNA isolation protocol of Passiflora ligularis. Materials and methods. Two methods of DNA extraction and two different tissuesof Passiflora ligularis were evaluated in terms of purity, quality and quantity of DNA yield, as well as DNA’s suitability for moleculartechniques based on PCR such as RAPDs. Quantification of DNA was done using absorbance spectrophotometry at a wavelength of260nm (A260 with a Beckman Du ® 530 spectrophotometer. An estimate of the DNA’s purity was obtained using the absorbance ratio(A260 / A280 nm. The variables analyzed in this study were the extraction method (A and the tissue type (B, in order to define their influenceon the purity and quantity of the DNA extracted. For the study of these variables a random design with a 2 x 2 factorial arrangement wasused. Results. The average quantities of DNA obtained with the modified method of Mc Couch et al. (1988 and the modified method ofDoyle & Doyle (1991 method were 778.9 μg/ml and 660.1 μg/ml respectively for dry tissue. Averages with fresh tissue were 1543.3 μg/ml and 820.4 μg/ml respectively. Conclusion. Based upon our results we suggest the use of Mc Couch et al. (1988 method with fresh leaftissue to obtain a high quality DNA suitable to be used with RAPDs molecular markers.

  14. Enhanced radiation response in radioresistant MCF-7 cells by targeting peroxiredoxin II

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    Diaz AJG

    2013-10-01

    Full Text Available Anthony Joseph Gomez Diaz,1 Daniel Tamae,2 Yun Yen,3 JianJian Li,4 Tieli Wang1 1Department of Chemistry and Biochemistry, California State University at Dominguez Hills, Carson, CA, 2Center of Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania, Philadelphia, PA, 3Department of Clinical and Molecular Pharmacology, Beckman Research Institute of City of Hope National Medical Center, Duarte, CA, 4Department of Radiation Oncology, University of California Davis, Sacramento, CA, USA Abstract: In our previous study, we identified that a protein target, peroxiredoxin II (PrxII, is overexpressed in radioresistant MCF+FIR3 breast-cancer cells and found that its expression and function is associated with breast-cancer radiation sensitivity or resistance. Small interference RNA (siRNA targeting PrxII gene expression was able to sensitize MCF+FIR3 radioresistant breast-cancer cells to ionizing radiation. The major focus of this work was to investigate how the radiation response of MCF+FIR3 radioresistant cells was affected by the siRNA that inhibits PrxII gene expression. Our results, presented here, show that silencing PrxII gene expression increased cellular toxicity by altering cellular thiol status, inhibiting Ca2+ efflux from the cells, and perturbing the intracellular Ca2+ homeostasis. By combining radiotherapy and siRNA technology, we hope to develop new therapeutic strategies that may have potential to enhance the efficacy of chemotherapeutic agents due to this technology's property of targeting to specific cancer-related genes. Keywords: siRNA, PrxII, radiation resistance, Ca2+, MCF+FIR3

  15. SNP genotypes of olfactory receptor genes associated with olfactory ability in German Shepherd dogs.

    Science.gov (United States)

    Yang, M; Geng, G-J; Zhang, W; Cui, L; Zhang, H-X; Zheng, J-L

    2016-04-01

    To find out the relationship between SNP genotypes of canine olfactory receptor genes and olfactory ability, 28 males and 20 females from German Shepherd dogs in police service were scored by odor detection tests and analyzed using the Beckman GenomeLab SNPstream. The representative 22 SNP loci from the exonic regions of 12 olfactory receptor genes were investigated, and three kinds of odor (human, ice drug and trinitrotoluene) were detected. The results showed that the SNP genotypes at the OR10H1-like:c.632C>T, OR10H1-like:c.770A>T, OR2K2-like:c.518G>A, OR4C11-like:c.511T>G and OR4C11-like:c.692G>A loci had a statistically significant effect on the scenting abilities (P dogs (P T, OR10H1-like:c.770A>T, OR4C11-like:c.511T>G and OR4C11-like:c.692G>A (P dogs with genotype CC at the OR10H1-like:c.632C>T, genotype AA at the OR10H1-like:c.770A>T, genotype TT at the OR4C11-like:c.511T>G and genotype GG at the OR4C11-like:c.692G>A loci did better at detecting the ice drug. We concluded that there was linkage between certain SNP genotypes and the olfactory ability of dogs and that SNP genotypes might be useful in determining dogs' scenting potential.

  16. Determination of standard enthalpies of formation and lattice energies of ammonium chloride and ammonium sulfate%氯化铵和硫酸铵生成焓与晶格能测定

    Institute of Scientific and Technical Information of China (English)

    杨春; 成文玉; 王庆伦; 刘晓莉; 王桂香; 杨津

    2013-01-01

    A new inorganic chemistry experiment of “Determination of standard enthalpies of formation and lattice energies of ammonium chloride and ammonium sulfate” has been designed . To reduce the measurement error , temperature was measured by digital Beckman thermometer and the experimental data were passed to computer for drawing and linear regress by means of software Origin . As a result , the mass of ammonium chloride used for measurement of enthalpy of dissolution decreased to about 40% .The designing experiment on ammonium sulfate can cultivate the integrated ability of students .Calculations of the lattice energies of ammonium chloride and ammonium sulfate based on their enthalpies of dissolution are introduced ,which can guide the students to research the relationship between the ionic crystal structures and physicochemical properties .%设计改进了一个新的无机化学实验“氯化铵和硫酸铵生成焓与晶格能的测定”。用数字贝克曼温度计代替水银温度计以减小测量的误差;用作图法对温度-时间曲线进行校正,提高了温度测量的准确度;在测定N H4 C1溶解焓的实验中使N H4 C1的用量减少为原来的40%,硫酸铵的设计性实验可以培养学生的综合能力,所得硫酸铵溶液可用作制备硫酸亚铁铵的原料;通过溶解热求晶格能,引导学生探究离子晶体结构与物性间的关系。

  17. The effect of leukocyte proof filter on coagulation factor and plasma protein in fresh frozen plasma%白细胞滤器对新鲜冰冻血浆凝血因子及血浆蛋白的影响

    Institute of Scientific and Technical Information of China (English)

    蔡杰; 胡俊妍; 陈映娥; 杨建鑫; 程本坤

    2006-01-01

    目的探讨经白细胞过滤器过滤后的新鲜冰冻血浆(Fresh frozen plasma, FFP)中凝血因子的生物活性及血浆蛋白含量变化. 方法随机抽取30份新鲜冰冻血浆, 置于37℃水浴融化,在超净工作台内留取过滤前后血浆样本各2ml, 使用Sysmex1500全自动血凝仪测定凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原(Fbg)、凝血因子Ⅶ (FⅦ:C)、凝血因子Ⅷ (FⅧ:C)、凝血因子Ⅸ(FⅨ:C)水平;用BECKMAN LX20全自动生化分析仪对TP、ALB进行检测. 结果过滤前后的新鲜冰冻血浆TT、 Fbg、FⅦ、FⅨ、TP、ALB水平的差异均无显著性(P>0.05).APTT、PT、FⅧ过滤前后的差异有显著性(P<0.05), 但仍在参考值范围内. 结论过滤前后新鲜冰冻血浆中凝血因子的活性差异变化及血浆蛋白的含量在参考值范围内, 适用于临床治疗.

  18. Power-free chip enzyme immunoassay for detection of prostate specific antigen (PSA) in serum.

    Science.gov (United States)

    Adel Ahmed, Heba; Azzazy, Hassan M E

    2013-11-15

    A power-free, portable "Chip EIA" was designed to render the popular Enzyme Linked Immunosorbent Assay (ELISA) more suitable for point-of-care testing. A number of microfluidic platforms have enabled miniaturization of the conventional microtitre plate ELISA, however, they require external pumping systems, valves, and electric power supply. The Chip EIA platform has eliminated the need for pumps and valves through utilizing a simple permanent magnet and magnetic nanoparticles. The magnetic nanoparticles act as solid support to capture the target and are then moved through chambers harboring different reagents necessary to perform a sandwich ELISA. The use of magnetic nanoparticles increases the volume-to-surface ratio reducing the assay time to 30 min. Changing the color of horseradish peroxidase (HRP) substrate to green indicates a positive result. In addition, a quantitative read-out was obtained through the use of cellphone camera imaging and analyzing the images using Matlab®. Cell phones, including smart ones, are readily available almost everywhere. The Chip EIA device was used to assay total prostate specific antigen (tPSA) in 19 serum samples. The PSA Chip EIA was tested for accuracy, precision, repeatability, and the results were correlated to the commercial Beckman Colter, Hybritech immunoassay® for determination of tPSA in serum samples with a Pearson correlation coefficient (R(2)=0.96). The lower detection limit of the PSA Chip EIA was 3.2 ng/mL. The assay has 88.9% recovery and good reproducibility (% CV of 6.5). We conclude that the developed Chip EIA can be used for detection of protein biomarkers in biological specimens.

  19. Total serum bile acid as a potential marker for the diagnosis of cholangiocarcinoma without jaundice.

    Science.gov (United States)

    Sombattheera, Sutthikan; Proungvitaya, Tanakorn; Limpaiboon, Temduang; Wongkham, Sopit; Wongkham, Chaisiri; Luvira, Vor; Proungvitaya, Siriporn

    2015-01-01

    Diagnosis of cholangiocarcinoma (CCA) is difficult when patients do not show jaundice. The aim of this study was to examine the feasibility of using the total serum bile acid (TSBA) level as an aid for the diagnosis of CCA in patients without jaundice. For this purpose, TSBA of the following groups were measured using a Beckman Synchron CX4 clinical chemistry analyzer: 60 cases of CCA with total serum bilirubin ≤2 mg/dL (low total bilirubin group, LTB); 32 cases of CCA with total serum bilirubin >2 mg/dL (high total bilirubin group, HTB); and 115 healthy controls. Liver function parameters such as serum cholesterol, albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were also examined. The results showed that the TSBA of both LTB and HTB groups of the CCA patients were significantly higher than that of the healthy controls. Also, significant correlation was observed between TSBA and total bilirubin levels in the HTB group of CCA patients. However, no such correlation was seen in the LTB group. The cut-off value of TSBA was determined for the LTB group of CCA patients using the receiver operating characteristic curve analysis, and it was 6.05 μmol/L with the sensitivity and specificity of 46.7% and 84.4%, respectively. In addition, the ALP level was correlated well with the TSBA level and ALP in HTB group was significantly higher than that of LTB group. Moreover, the combination of high TSBA and high ALP levels gave higher specificity up to 97.4%. TSBA might be useful for the diagnosis of CCA patients without jaundice.

  20. The Putative Role of the Non-Gastric H+/K+-ATPase ATP12A (ATP1AL1 as Anti-Apoptotic Ion Transporter: Effect of the H+/K+ ATPase Inhibitor SCH28080 on Butyrate-Stimulated Myelomonocytic HL-60 Cells

    Directory of Open Access Journals (Sweden)

    Martin Jakab

    2014-10-01

    Full Text Available Background/Aims: The ATP12A gene codes for a non-gastric H+/K+ ATPase, which is expressed in a wide variety of tissues. The aim of this study was to test for the molecular and functional expression of the non-gastric H+/K+ ATPase ATP12A/ATP1AL1 in unstimulated and butyrate-stimulated (1 and 10 mM human myelomonocytic HL-60 cells, to unravel its potential role as putative apoptosis-counteracting ion transporter as well as to test for the effect of the H+/K+ ATPase inhibitor SCH28080 in apoptosis. Methods: Real-time reverse-transcription PCR (qRT-PCR was used for amplification and cloning of ATP12A transcripts and to assess transcriptional regulation. BCECF microfluorimetry was used to assess changes of intracellular pH (pHi after acute intracellular acid load (NH4Cl prepulsing. Mean cell volumes (MCV and MCV-recovery after osmotic cell shrinkage (Regulatory Volume Increase, RVI were assessed by Coulter counting. Flow-cytometry was used to measure MCV (Coulter principle, to assess apoptosis (phosphatidylserine exposure to the outer leaflet of the cell membrane, caspase activity, 7AAD staining and differentiation (CD86 expression. Results: We found by RT-PCR, intracellular pH measurements, MCV measurements and flow cytometry that ATP12A is expressed in human myelomonocytic HL-60 cells. Treatment of HL-60 cells with 1 mM butyrate leads to monocyte-directed differentiation whereas higher concentrations (10 mM induce apoptosis as assessed by flow-cytometric determination of CD86 expression, caspase activity, phosphatidylserine exposure on the outer leaflet of the cell membrane and MCV measurements. Transcriptional up-regulation of ATP12A and CD86 is evident in 1 mM butyrate-treated HL-60 cells. The H+/K+ ATPase inhibitor SCH28080 (100 µM diminishes K+-dependent pHi recovery after intracellular acid load and blocks RVI after osmotic cell shrinkage. After seeding, HL-60 cells increase their MCV within the first 24 h in culture, and subsequently

  1. Sources of SOA gaseous precursors in contrasted urban environments: a focus on mono-aromatic compounds and intermediate volatility compounds

    Science.gov (United States)

    Salameh, Therese; Borbon, Agnès; Ait-Helal, Warda; Afif, Charbel; Sauvage, Stéphane; Locoge, Nadine; Bonneau, Stéphane; Sanchez, Olivier

    2016-04-01

    , SP95 E10, and SP98) and was used to constraint evaporative emissions in order to predict the headspace vapour composition (Harley and Coulter-Burke, 2000). Modelled and observed compositions are in good agreement (differences up to 20%). Therefore, the implemented model is a relevant tool to test the sensitivity of BTEX and other VOCs ambient composition to evaporative emissions of fuels with regards to their composition. Such analysis will be extended to other target cities and similarities/differences will be presented regarding regional characteristics. This work was supported by the Ile de France region, Life and PHOTOPAQ grant, PICS-CNRS, ENVIMED and ChArMEx. We would like to thank Laurence Dépelchin and Thierry Léonardis for technical support and AIRPARIF for providing the data. Borbon, A., et al. (2013) Emission ratios of anthropogenic VOC in northern mid-latitude megacities: observations vs. emission inventories in Los Angeles and Paris, J. Geophys. Res. 118, 2041 - 2057. Harley, R. and Coulter-Burke, S. (2000) Relating Liquid Fuel and Headspace Vapor Composition for California Reformulated Gasoline Samples Containing Ethanol, Environ. Sci. Technol. 34, 4088-4094. Ait-Helal, W.; Borbon, A.; Sauvage, S.; et al., Atmos. Chem. Phys. vol. 14 , No. 19 , p. 10439-10464

  2. Electrical cell counting process characterization in a microfluidic impedance cytometer.

    Science.gov (United States)

    Hassan, Umer; Bashir, Rashid

    2014-10-01

    Particle counting in microfluidic devices with coulter principle finds many applications in health and medicine. Cell enumeration using microfluidic particle counters is fast and requires small volumes of sample, and is being used for disease diagnostics in humans and animals. A complete characterization of the cell counting process is critical for accurate cell counting especially in complex systems with samples of heterogeneous population interacting with different reagents in a microfluidic device. In this paper, we have characterized the electrical cell counting process using a microfluidic impedance cytometer. Erythrocytes were lysed on-chip from whole blood and the lysing was quenched to preserve leukocytes which subsequently pass through a 15 μm × 15 μm measurement channel used to electrically count the cells. We show that cell counting over time is a non-homogeneous Poisson process and that the electrical cell counts over time show the log-normal distribution, whose skewness can be attributed to diffusion of cells in the buffer that is used to meter the blood. We further found that the heterogeneous cell population (i.e. different cell types) shows different diffusion characteristics based on the cell size. Lymphocytes spatially diffuse more as compared to granulocytes and monocytes. The time difference between the cell occurrences follows an exponential distribution and when plotted over time verifies the cell diffusion characteristics. We also characterized the probability of occurrence of more than one cell at the counter within specified time intervals using Poisson counting statistics. For high cell concentration samples, we also derived the required sample dilution based on our particle counting characterization. Buffer characterization by considering the size based particle diffusion and estimating the required dilution are critical parameters for accurate counting results.

  3. ELEMENTS OF MAINTENANCE MANAGEMENT IN PUBLIC INSTITUTIONS OF HIGHER EDUCATION CABIMAS MUNICIPALITY

    Directory of Open Access Journals (Sweden)

    Ricardo Castillo

    2013-07-01

    Full Text Available This article is an advance of a wider investigation, in which the objective is to analyze overall maintenance management in public institutions of higher education in the municipality Cabimas, hence in this development describes the elements of the management maintenance present in these institutions. Authors were consulted such as Duffuaa et al. (2010, Clemenza (2010, Zambrano and Leal (2006 , Robbins and Coulter (2005 y Martínez (2007 among others. The research is descriptive, non-experimental   design, transactional  and  field.  We  used  a population   census,  for  a  total  of   twenty-three  (23   research  subjects,  belonging  to  the Universidad   del   Zulia    Núcleo   Costa   Oriental   del   Lago,  Universidad    Nacional Experimental  Rafael  Maria Baralt and  Instituto Universitario Tecnológico  Cabimas. To collect data, a questionnaire was composed of twenty-two (22 items, which was validated by the trial of six (6 experts, with 0.99 reliability by Cronbach alpha coefficient.  We conclude that the elements planning, organizing, scheduling, execution and control, have a high presence in maintenance management, not the human resource and security elements.

  4. Infection-stimulated Anemia Results Primarily from Interferon Gamma-dependent, Signal Transducer and Activator of Transcription 1-independent Red Cell Loss

    Institute of Scientific and Technical Information of China (English)

    Zheng Wang; Dong-Xia Zhang; Qi Zhao

    2015-01-01

    Background:Although the onset of anemia during infectious disease is commonly correlated with production of inflammatory cytokines,the mechanisms by which cytokines induce anemia are poorly defined.This study focused on the mechanism research.Methods:Different types of mice were infected perorally with Toxoplasma gondii strain ME49.At the indicated times,samples from each mouse were harvested,processed,and analyzed individually.Blood samples were analyzed using a Coulter Counter and red blood cell (RBC) survival was measured by biotinylation.Levels of tumor necrosis factor-α (TNF-α),inducible nitric oxide synthase (iNOS),and inducible protein 10 (IP-10) mRNA in liver tissue were measured by real-time polymerase chain reaction.Results:T.gondii-infected mice exhibited anemia due to a decrease in both erythropoiesis and survival time of RBC in the circulation (P < 0.02).In addition,infection-stimulated anemia was associated with fecal occult,supporting previous literature that hemorrhage is a consequence of T.gondii infection in mice.Infection-induced anemia was abolished in interferon gamma (IFNγ) and IFNγ receptor deficient mice (P < 0.05) but was still evident in mice lacking TNF-α,iNOS,phagocyte NADPH oxidase or IP-10 (P < 0.02).Neither signal transducer and activator of transcription 1 (STAT1) deficient mice nor 129S6 controls exhibited decreased erythropoiesis,but rather suffered from an anemia resulting solely from increased loss of circulating RBC.Conclusions:Infection-stimulated decrease in erythropoiesis and losses of RBC have distinct mechanistic bases.These results show that during T.gondii infection,IFNγ is responsible for an anemia that results from both a decrease in erythropoiesis and a STAT1 independent loss of circulating RBC.

  5. Feasibility of using a particle counter or flow-cytometer for bacterial enumeration in the assimilable organic carbon (AOC) analysis method.

    Science.gov (United States)

    Aggarwal, Srijan; Jeon, Youchul; Hozalski, Raymond M

    2015-09-01

    Assimilable organic carbon (AOC) is one of the major determinants of microbial growth and stability in drinking water distribution systems. Nevertheless, AOC measurements are rarely conducted in practice owing, in part, to the tedious and time-consuming nature of the bioassay. Herein, we compared three alternative cell count approaches [flow cytometry with staining (FC-S), flow cytometry without staining (FC-NS), and particle counting (Coulter counter; CC)] for bacterial enumeration as a means to expedite the AOC bioassay. Our results suggest that of the three methods only FC-S provides a suitable alternative to plate counting for rapid and accurate enumeration of both P17 and NOX in the AOC bioassay. While the cell counts obtained by FC-NS were linearly correlated with those obtained using the traditional heterotrophic plate count (HPC) method (FC-NS: R(2) = 0.89-0.96), the AOC values obtained by FC-NS were overestimated by 18-57 %. The CC approach was unsuccessful in enumerating Spirillum strain NOX cells because of the relatively small size of that organism. The CC counts were linearly correlated with HPC for Pseudomonas fluorescens strain P-17 (P17) cells (R(2) = 0.83) but like FC-NS, the CC approach also overestimated the AOC values (for P-17). The advantage of the FC-S method over the other two is improved sensitivity and the ability to specifically enumerate whole cells (and likely viable) as opposed to non-viable cells, cell debris, and other contaminating particles introduced by the test water itself or sample handling.

  6. Classification of biological cells using a sound wave based flow cytometer

    Science.gov (United States)

    Strohm, Eric M.; Gnyawali, Vaskar; Van De Vondervoort, Mia; Daghighi, Yasaman; Tsai, Scott S. H.; Kolios, Michael C.

    2016-03-01

    A flow cytometer that uses sound waves to determine the size of biological cells is presented. In this system, a microfluidic device made of polydimethylsiloxane (PDMS) was developed to hydrodynamically flow focus cells in a single file through a target area. Integrated into the microfluidic device was an ultrasound transducer with a 375 MHz center frequency, aligned opposite the transducer was a pulsed 532 nm laser focused into the device by a 10x objective. Each passing cell was insonfied with a high frequency ultrasound pulse, and irradiated with the laser. The resulting ultrasound and photoacoustic waves from each cell were analyzed using signal processing methods, where features in the power spectra were compared to theoretical models to calculate the cell size. Two cell lines with different size distributions were used to test the system: acute myeloid leukemia cells (AML) and melanoma cells. Over 200 cells were measured using this system. The average calculated diameter of the AML cells was 10.4 +/- 2.5 μm using ultrasound, and 11.4 +/- 2.3 μm using photoacoustics. The average diameter of the melanoma cells was 16.2 +/- 2.9 μm using ultrasound, and 18.9 +/- 3.5 μm using photoacoustics. The cell sizes calculated using ultrasound and photoacoustic methods agreed with measurements using a Coulter Counter, where the AML cells were 9.8 +/- 1.8 μm and the melanoma cells were 16.0 +/- 2.5 μm. These results demonstrate a high speed method of assessing cell size using sound waves, which is an alternative method to traditional flow cytometry techniques.

  7. Markers of inflammation are inversely associated with VO2 max in asymptomatic men.

    Science.gov (United States)

    Kullo, Iftikhar J; Khaleghi, Mahyar; Hensrud, Donald D

    2007-04-01

    We investigated whether markers of inflammation, including a cytokine (IL-6), acute-phase reactants [C-reactive protein (CRP) and fibrinogen], and white blood cell (WBC) count are associated with maximal O(2) consumption (Vo(2 max)) in men without coronary heart disease (CHD). In asymptomatic men (n = 172, 51 +/- 9.3 yr old), Vo(2 max) was measured during a symptom-limited graded treadmill exercise test. Physical activity level was assessed by a standardized questionnaire. IL-6 and CRP were measured by immunoassays, fibrinogen by the Clauss method, and WBC count with a Coulter counter. IL-6 and CRP were logarithmically transformed to reduce skewness. Multivariable regression was used to assess whether markers of inflammation were associated with Vo(2 max) after adjustment for age, body mass index, CHD risk factors, and lifestyle variables (physical activity level, percent body fat, and alcohol intake). Vo(2 max) was 34.5 ml.kg(-1).min(-1) (SD 6.1). Log IL-6 (r = -0.38, P max). In separate multivariable linear regression models that adjusted for age, body mass index, CHD risk factors, and lifestyle variables, log IL-6 [beta-coeff = -1.66 +/- 0.63 (SE), P = 0.010], log CRP [beta-coeff = -0.99 +/- 0.33 (SE), P = 0.003], fibrinogen [beta-coeff = -1.51 +/- 0.44 (SE), P = 0.001], and WBC count [beta-coeff = -0.52 +/- 0.30 (SE), P = 0.088] were each inversely associated with Vo(2 max). In conclusion, higher circulating levels of IL-6, CRP, and fibrinogen are independently associated with lower Vo(2 max) in asymptomatic men.

  8. Prevalence of Anemia and Associated Factors in Child Bearing Age Women in Riyadh, Saudi Arabia

    Science.gov (United States)

    AlQuaiz, AlJohara M.; Gad Mohamed, Ashry; Khoja, Tawfik A. M.; AlSharif, Abdullah; Shaikh, Shaffi Ahamed; Al Mane, Hamad; Aldiris, Abdallah; Hammad, Durdana

    2013-01-01

    Objective. To determine the prevalence and risk factors for anemia in child bearing age women in Riyadh, Saudi Arabia. Design. Cross-sectional survey was conducted using two-stage cluster sampling. 25 clusters (primary health care centers (PHCC)) were identified from all over Riyadh, and 45–50 households were randomly selected from each cluster. Eligible women were invited to PHCC for questionnaire filling, anthropometric measurements, and complete blood count. Blood hemoglobin was measured with Coulter Cellular Analysis System using light scatter method. Setting. PHCC. Subjects. 969 (68%) women out of 1429 women were included in the analysis. Results. Mean hemoglobin was 12.35 (±1.80) g/dL, 95% CI 12.24–12.46 with interquartile range of 1.9. Anemia (Hb <12 g/dL) was present in 40% (390) women. Mean (±SD) for MCH, MCV, MCHC, and RDW was 79.21 (±12.17) fL, 26.37 (±6.21) pg, 32.36 (±4.91) g/dL, and 14.84 (±4.65)%, respectively. Multivariate logistic regression revealed that having family history of iron deficiency anemia (OR 2.91, 95% CI 1.78–4.76) and infrequent intake of meat (OR 1.54, 95%CI 1.15–2.05) were associated with increased risk of anemia, whereas increasing body mass index (OR 0.95, 95% CI 0.92–0.97) was associated with reduced risk of anemia. Conclusion. Women should be educated about proper diet and reproductive issues in order to reduce the prevalence of anemia in Saudi Arabia. PMID:24205435

  9. Prevalence of Anemia and Associated Factors in Child Bearing Age Women in Riyadh, Saudi Arabia

    Directory of Open Access Journals (Sweden)

    AlJohara M. AlQuaiz

    2013-01-01

    Full Text Available Objective. To determine the prevalence and risk factors for anemia in child bearing age women in Riyadh, Saudi Arabia. Design. Cross-sectional survey was conducted using two-stage cluster sampling. 25 clusters (primary health care centers (PHCC were identified from all over Riyadh, and 45–50 households were randomly selected from each cluster. Eligible women were invited to PHCC for questionnaire filling, anthropometric measurements, and complete blood count. Blood hemoglobin was measured with Coulter Cellular Analysis System using light scatter method. Setting. PHCC. Subjects. 969 (68% women out of 1429 women were included in the analysis. Results. Mean hemoglobin was 12.35 (±1.80 g/dL, 95% CI 12.24–12.46 with interquartile range of 1.9. Anemia (Hb <12 g/dL was present in 40% (390 women. Mean (±SD for MCH, MCV, MCHC, and RDW was 79.21 (±12.17 fL, 26.37 (±6.21 pg, 32.36 (±4.91 g/dL, and 14.84 (±4.65%, respectively. Multivariate logistic regression revealed that having family history of iron deficiency anemia (OR 2.91, 95% CI 1.78–4.76 and infrequent intake of meat (OR 1.54, 95%CI 1.15–2.05 were associated with increased risk of anemia, whereas increasing body mass index (OR 0.95, 95% CI 0.92–0.97 was associated with reduced risk of anemia. Conclusion. Women should be educated about proper diet and reproductive issues in order to reduce the prevalence of anemia in Saudi Arabia.

  10. On the relationship between band broadening and the particle-size distribution of the packing material in liquid chromatography: theory and practice.

    Science.gov (United States)

    Gritti, Fabrice; Farkas, Tivadar; Heng, Josuah; Guiochon, Georges

    2011-11-11

    The influence of the particle size distribution (PSD) on the band broadening and the efficiency of packed columns is investigated on both theoretical and practical viewpoints. Each of the classical contributions to mass transfer kinetics, those due to longitudinal diffusion, eddy dispersion, and solid-liquid mass transfer resistance are measured and analyzed in terms of their expected and observed intensity as a function of the PSD of mixtures of the commercially available packing materials, 5 and 3 μm Luna-C₁₈ particles (Phenomenex, Torrance, CA, USA). Six 4.6 mm × 150 mm columns were packed with different mixtures of these two materials. The efficiencies of these columns were measured for a non-retained and a retained analytes in a mixture of acetonitrile and water. The longitudinal diffusion coefficient was directly measured by the peak parking method. The solid-liquid mass transfer coefficient was measured from the combination of the peak parking method, the best model of effective diffusion coefficient and the actual PSDs of the different particle mixtures measured by Coulter counter experiments. The eddy diffusion term was measured according to a recently developed protocol, by numerical integration of the peak profiles. Our results clearly show that the PSD has no measurable impact on any of the coefficients of the van Deemter equation. On the contrary and surprisingly, adding a small fraction of large particles to a batch of small particles can improve the quality of the packing of the fine particles. Our results indirectly confirm that the success of sub-3 μm shell particles is due to the roughness of their external surface, which contributes to eliminate most of the nefarious wall effects.

  11. Quantificação de células CD 34+ em sangue periférico, produto de aférese e cordão umbilical: estudo comparativo de três diferentes metodologias

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    Bacal Nydia S.

    2001-01-01

    Full Text Available A quantificação das células CD34+ em sangue periférico é utilizada para determinar o melhor momento de iniciar a aférese, enquanto que na leucoaférese e no sangue de cordão umbilical determinam a quantidade de células CD34+ para o transplante de células progenitoras. O objetivo deste trabalho foi comparar três diferentes metodologias de quantificação de células CD34+. Foram utilizados três diferentes tipos de amostras: a 32 amostras de sangue periférico, coletadas de pacientes estimulados com G-CSF 50 mg/Kg/dose total, sem quimioterapia na mobilização. b 31 amostras de produto de aférese de pacientes estimulados com o mesmo protocolo de G-CSF. c 20 amostras de sangue de cordão coletadas em CPDA-1, por punção de veia umbilical. As amostras permaneceram à temperatura ambiente no máximo até 24h da análise. O citômetro de fluxo utilizado foi o Epics XL-MCL (Coulter com os protocolos de dupla plataforma: ISHAGE e Mulhouse modificado para análise de maior numero de eventos, e o citômetro Imagn 2000 (Biometric Imaging de plataforma única, conforme técnicas recomendadas. Os anticorpos monoclonais utilizados foram: CD45-FITC, CD34-PE, e isotipo IgG1-PE da Immunotech. As análises estatísticas foram: ANOVA e correlação de teste t de Student. Os resultados não apresentaram diferenças estatisticamente significativas nos três métodos.

  12. Spatial variation in the community of insects associated with the flowers of Pachycereus weberi (Caryophyllales: Cactaceae).

    Science.gov (United States)

    Figueroa-Castro, Dulce María; Valverde, Pedro Luis; Vite, Fernando; Carrillo-Ruiz, Hortensia

    2014-08-01

    The positive relationship between productivity and species diversity is well-known. Insect communities associated with the flowers of Cactaceae species represent an interesting system to explore the productivity-diversity relationship because branches facing the equator receive more photosynthetically active radiation and have higher productivity. Thus, flowers with contrasting orientations within an individual, and even within a single branch, might differ in productivity. Therefore, higher abundance, species richness, and diversity are expected for the insect communities associated with south-facing flowers. This hypothesis was tested in Pachycereus weberi (J.M. Coulter) Backeberg (Cactaceae). Insects within flowers with contrasting orientations were collected and its abundance, richness, and diversity were estimated. We also asked if insects prefer big flowers. Thus, flower volume was estimated and regression analyses were conducted to test if there is a positive relationship between flower size and insect abundance. Flower orientation did not affect species richness. However, species abundance and diversity were different in flowers with contrasting orientations. In general, species abundance was higher in flowers facing southwards than in north-facing flowers. On the contrary, species diversity was higher in north-facing flowers. Abundance of Coleoptera was explained by flower volume in south-facing flowers. Contrary to our hypothesis, total diversity was greater in the less productive oriented flowers. Three possible explanations are discussed to explain the low diversity found in the highly productive, south-facing flowers. Our study provides evidence for the effects of productivity on the structure of insect communities at a very small-scale.

  13. Prevalence of Common Hemoglobinopathies in Gujarat: An Analysis of a Large Population Screening Program

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    Patel Ashwin P, Naik Madhuben R, Shah Nilam M, Sharma Narmadeshwar P, Parmar Prakash H

    2012-01-01

    Full Text Available Objectives: To determine prevalence and geographical distribution of ß-thalassemia trait and sickle cell trait in Gujarat Methods: The present study includes 3, 17,539 persons who were screened for hemoglobinopathies from September 2004 to November 2008. Blood samples were collected into EDTA and run in coulter AC.T diff 2 TM, electronic analyzer and analyzed on Bio-Rad Variant I HPLC System. Results: The prevalence of ß-thalassemia trait (BTM and sickle cell trait (SCT is 1.95% and 6.54% respectively. In tribal area, 1.74% has BTM and 11.37% has SCT trait while in non-tribal area, BTM is seen in 2.18% and 1.1% has SCT. Rathva (18.65%, Nayka (15.38%, Gamit (17.64%, Tadvi (16.95%, Bariya (16.5%, Adivasi (15.25% and Chaudhary (15.63% communities have higher prevalence of SCT. BTM is more prevalent in Bhanusali (8.1%, Bhakta (7.93% and Luhana (6.5% community. SCT is very common in schedule castes (8.1-10.52 % and it is seen in many general castes. Conclusion: The prevalence of BTM is 1.95% while SCT is 6.54% in Gujarat. Sickle cell gene frequency is high in tribals (11.37 % except in Banaskantha (5.12 % and Sabarkantha (3.43%. Sickle cell is prevalent in all schedule castes and many general castes. ß-thalassemia gene frequency is higher in Sindhi (Bhanusali and Bhakta communities and in Banaskantha district (4.45%.

  14. MicroRNA-152 regulates DNA methyltransferase 1 and is involved in the development and lactation of mammary glands in dairy cows.

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    Jie Wang

    Full Text Available MicroRNAs (miRNAs are a class of small non-coding, endogenous regulatory RNAs that function by controlling gene expression at the post-transcriptional level. Using small RNA sequencing and qRT-PCR techniques, we found that the expression of miR-152 was significantly increased during lactation in the mammary glands of dairy cows producing high quality milk compared with that in cows producing low quality milk. Furthermore, DNA methyltransferase 1 (DNMT1, which is a target of miR-152, was inversely correlated with the expression levels of miR-152 in the mammary glands of dairy cows. Dairy cow mammary epithelial cells (DCMECs were used as in vitro cell models to study the function of miR-152. The forced expression of miR-152 in DCMECs resulted in a marked reduction of DNMT1 at both mRNA and protein levels. This in turn led to a decrease in global DNA methylation and increased the expression of two lactation-related genes, serine/threonine protein kinase Akt (Akt and peroxisome proliferator-activated receptor gamma (Pparγ. In contrast, inhibition of miR-152 showed the opposite results. By using an electronic Coulter counter (CASY-TT and flow cytometer, we discovered that miR-152 enhanced the viability and multiplication capacity of DCMECs. In conclusion, miR-152 plays an important role in the development and lactation processes in the mammary glands of dairy cows. Our data provide insights into dairy cow mammary gland development and lactation.

  15. PSA Isoforms' Velocities for Early Diagnosis of Prostate Cancer.

    Science.gov (United States)

    Heidegger, Isabel; Klocker, Helmut; Pichler, Renate; Horninger, Wolfgang; Bektic, Jasmin

    2015-06-01

    Free prostate-specific antigen (fPSA) and its molecular isoforms are suggested for enhancement of PSA testing in prostate cancer (PCa). In the present study we evaluated whether PSA isoforms' velocities might serve as a tool to improve early PCa diagnosis. Our study population included 381 men who had undergone at least one ultrasound-guided prostate biopsy whose pathologic examination yielded PCa or showed no evidence of prostatic malignancy. Serial PSA, fPSA, and proPSA measurements were performed on serum samples covering 7 years prior to biopsy using Beckmann Coulter Access immunoassays. Afterwards, velocities of PSA (PSAV), fPSA% (fPSA%V), proPSA% (proPSA%V) and the ratio proPSA/PSA/V were calculated and their ability to discriminate cancer from benign disease was evaluated. Among 381 men included in the study, 202 (53%) were diagnosed with PCa and underwent radical prostatectomy at our Department. PSAV, fPSA%V, proPSA%V as well as proPSA/PSA/V were able to differentiate significantly between PCa and non-cancerous prostate. The highest discriminatory power between cancer and benign disease has been observed two and one year prior to diagnosis with all measured parameters. Among all measured parameters, fPSA%V showed the best cancer specificity of 45.3% with 90% of sensitivity. In summary, our results highlight the value of PSA isoforms' velocity for early detection of PCa. Especially fPSA%V should be used in the clinical setting to increase cancer detection specificity.

  16. Correlation of Serum Concentrations of Soluble Thrombomodulin, Soluble Vascular Cell Adhesion Molecule-1,Intracellular Adhesion Molecule -1 And E-Selectin In Patients WithSystemic Lupus Erythematosus

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    Malak., A. Mohsen*, Magda.A.Gamil*,Maha. I.Shehata

    2003-09-01

    Full Text Available To date no specific serological parameters are available to assess disease activity in systemic lupus erythematosus (SLE. The objective of this study was to correlate serum levels of thrombomodulin (TM, intracellular adhesion molecule-1 sICAM-1, vascular cell adhesion molecule-1 sVCAM-1, and E-selectin with standard laboratory tests and clinical indices of disease activity in 40 patients with SLE and 20 apparently healthy persons as controls. According to British Isles Lupus Assessment Group (BILAG disease activity index, the 40 patients were divided into two groups, the first consisted of 22 with active disease, and the second consisted of 18 patients with inactive SLE. Serum sTM, sICAM-1, sVCAM-I, and E-selectin were measured in their sera, using enzyme linked immuonosorbent assay (ELISA technique.C-reactiv protein (CRP, Erythrocyte sedimentation rates (ESR and serum creatinines were measured by standard laboratory tests. Total leukocytic count and hemoglobin concentration were detected by coulter counter. Levels of sTM and sVCAM were highly elevated in the group of patients with active SLE as compared to the inactive one (P0.05. In SLE, the BILAG disease activity index, ESR and serum creatinine correlated best with sTM, sVCAM-1 and E-selectin levels while there was a weak association between CRP levels and the adhesion molecules, and no correlation between CRP level and disease activity. In conclusion, sTM and sVCAM were the most important serological indices of disease activity in SLE and might be valuable serological parameters for monitoring therapy.

  17. Soybean Tillage Systems and Physical Changes in Surface Layers of Two Albaqualf Soils

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    Gerson Meneghetti Sarzi Sartori

    Full Text Available ABSTRACT A compacted subsurface soil layer can be a limiting factor for soybean growing, reducing soybean yield. The aim of this study was to evaluate the effect of different tillage systems on the physical properties of two Albaqualf soils of the Central Plains region in the state of Rio Grande do Sul in southern Brazil. Two experiments were conducted: one in Santa Maria, RS, during the 2013/14 and 2014/15 crop seasons, and another in Formigueiro, RS, during the 2013/14 crop season. A randomized block experimental design with four replications was used. The treatments were: sowing using an offset double disc (T1; sowing using a fluted coulter disc (wavy disc with 12 waves (T2; sowing with a knife runner opener (T3; sowing with a knife runner opener + press wheel mechanism for ground levelling (T4; sowing using a furrow opener upon a raised bed (T5; and chisel plough + sowing using an offset double-disc (T6. In the 2014/15 growing season, the T4 factor was changed using a knife runner opener 0.05 m from the planting row. A smaller reduction in the compacted subsurface soil layer was observed for both T1 and T2, which exhibited high soil bulk density values for the 2013/14 and 2014/15 crop seasons. Furthermore, T3, T5 and T6 led to a reduction in bulk density, and increasing total porosity and macroporosity in the soil, which consequently increased water infiltration, water storage capacity, and crop yield in areas with the presence of a compacted subsurface soil layer.

  18. Membrane permeability of the human granulocyte to water, dimethyl sulfoxide, glycerol, propylene glycol and ethylene glycol.

    Science.gov (United States)

    Vian, Alex M; Higgins, Adam Z

    2014-02-01

    Granulocytes are currently transfused as soon as possible after collection because they rapidly deteriorate after being removed from the body. This short shelf life complicates the logistics of granulocyte collection, banking, and safety testing. Cryopreservation has the potential to significantly increase shelf life; however, cryopreservation of granulocytes has proven to be difficult. In this study, we investigate the membrane permeability properties of human granulocytes, with the ultimate goal of using membrane transport modeling to facilitate development of improved cryopreservation methods. We first measured the equilibrium volume of human granulocytes in a range of hypo- and hypertonic solutions and fit the resulting data using a Boyle-van't Hoff model. This yielded an isotonic cell volume of 378 μm(3) and an osmotically inactive volume of 165 μm(3). To determine the permeability of the granulocyte membrane to water and cryoprotectant (CPA), cells were injected into well-mixed CPA solution while collecting volume measurements using a Coulter Counter. These experiments were performed at temperatures ranging from 4 to 37°C for exposure to dimethyl sulfoxide, glycerol, ethylene glycol, and propylene glycol. The best-fit water permeability was similar in the presence of all of the CPAs, with an average value at 21°C of 0.18 μmatm(-1)min(-1). The activation energy for water transport ranged from 41 to 61 kJ/mol. The CPA permeability at 21°C was 6.4, 1.0, 8.4, and 4.0 μm/min for dimethyl sulfoxide, glycerol, ethylene glycol, and propylene glycol, respectively, and the activation energy for CPA transport ranged between 59 and 68 kJ/mol.

  19. CALIX[4]ARENE C-145 EFFECTS ON СELLULAR HAEMOSTASIS

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    V. O. Chernyshenko

    2016-06-01

    Full Text Available The aim of the research was to study a potential antithrombotic sodium salt of calix[4]arene-methylene-bis-phosphonic acid (С-145 — on activation and aggregation of platelets in vivo, as well as on proliferation and apoptosis of endothelial cells in the cell culture. Effects of calix[4]arene С-145 estimated in vitro after addition to the platelet rich plasma, and in vivo after intravenous injection into rabbit bloodstream in equivalent amounts (46 μM. Aggregation of platelets was induced by adenosine diphosphate and detected using aggregometer Solar AP2110. Platelet shape and cytoplasmic granularity were monitored on COULTER EPICS XL Flow Cytometer. The level of tissuetype plasminogen activator — tPA — was estimated using enzyme-linked immunosorbent assay ELISA. Effects of calix[4]arene C-145 on culture of endotelial cells cells was studied using 3-(4,5-Dimethylthiazol-2-yl-2,5-Diphenyltetrazolium Bromide — MTT-test. The population of proliferative pool of cells (G2/M+S was determined using flow cytometry. Aggregometry and flow cytometry showed that calix[4]arene C-145 did not activate platelets nor affect their aggregation in vitro. However intravenous injection of calix[4]arene C-145 into the bloodstream of healthy rabbits leads to strong inhibition of platelet aggregation and changes of shape and granularity of most of the platelets after 2 hours of administration. Any additional appearance of endothelial cells activation marker tPA in vivo and any inhibition of calix[4]arene C-145 on proliferation of endothelial cells in cell culture did not observe. So calix[4]arene C-145 had strong anti-platelet effect in vivo that was not a result of their direct action on platelets or endothelial cells in vitro. This allowed to assume the possibility of calix[4]arene C-145 use as an effective antithrombotic agent.

  20. La complejidad de las organizaciones: aproximación a un modelo teórico

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    Gerardo J. Zapata Rotundo

    2008-01-01

    Full Text Available Este artículo propone un modelo teórico sobre la complejidad de las organizaciones, teniendo como marco de referencia los constructos complejidad cognitiva y complejidad relacional. La complejidad relacional (Hall et al., 1967; Boisot y Child, 1999; Hall, 1996; Robbins y Coulter, 2002, se vincula con los procesos de diferenciación e integración que se dan en las organizaciones, y se explica fundamentalmente a través del comportamiento y gestión de las variables de diseño organizativo. Por su parte, la complejidad cognitiva (Perrow, 1970; Boisot y Child, 1999; Peris et al., 2001, está asociada con la dificultad de comprender y llevar a cabo los aspectos más esenciales del trabajo realizado en la organización; de ahí, la presencia de diferentes tipos de trabajo con distintos grados de dificultad y variedad (naturaleza del trabajo. El modelo sirve de base a tres proposiciones que se señalan en las consideraciones finales de la investigación, donde se plantean un conjunto de relaciones entre sus elementos constituyentes: tipos básicos de trabajo, variables de diseño (centralización y formalización, complejidad cognitiva y relacional, estilo de liderazgo y forma organizativa que adopta la empresa. Por último, se pretende con el modelo propuesto contribuir al esfuerzo de seguir comprendiendo el diseño de las organizaciones y su comportamiento, y, por tanto, a favor de la formulación de nuevas soluciones a sus problemas.

  1. Evaluation of the S phase distribution of flow cytometric DNA histograms by autoradiography and computer algorithms.

    Science.gov (United States)

    Sheck, L E; Muirhead, K A; Horan, P K

    1980-09-01

    Cell sorting and tritiated thymidine autoradiography were used to define the distribution of S phase cells in flow cytometric DNA histograms obtained from exponential mouse lymphoma cells (L5178Y). The numbers of labeled S phase cells, autoradiographically determined from cells sorted at 2-channel intervals in the G1/early S and late S/G2M regions of the histogram, were compared with the numbers of computed S phase cells in comparable 2-channel intervals as predicted by several computer algorithms used to extract cell cycle phase distributions from DNA histograms. Polynomial and multirectangle algorithms gave computed estimates of total %S in close agreement with the tritiated thymidine labeling index for the cell population, while multi-Gaussian algorithms underestimated %S. Interval autoradiographic and algorithm studies confirmed these results in that no significant differences were found between the autoradiographic S phase distribution and S phase distributions calculated by the polynomial and multirectangle models. However, S phase cells were significantly underestimated in G1/early S by a constrained multi-Gaussian model and in both G1/early S and late S/G2 by an unconstrained multi-Gaussian model. For the particular cell line (L5178Y), staining protocol (mithramycin following ethanol fixation) and instrumentation (Coulter TPS-2 cell sorter) used in this study, close agreement between computed %S and tritiated thymidine labeling index was found to be a reliable indicator of an algorithm's success in resolving S phase cells in the G1/S and S/G2 transition regions of the DNA histograms.

  2. Identifying the AD 1257 Salamas volcanic event from micron-size tephra composition in two East Antarctic ice cores

    Science.gov (United States)

    Petit, Jean Robert; Narcisi, Biancamaria; Batanova, Valentina G.; Joël, Savarino; Komorowski, Jean Christophe; Michel, Agnes; Metrich, Nicole; Besson, Pascale; Vidal, Celine; Sobolev, Alexander V.

    2016-04-01

    A wealth of valuable data about the history of explosive volcanic history can be extracted from polar ice successions. Both the volatile by-products and the solid silicate (tephra) components of volcanic plumes can be incorporated into snow layers, providing tools for chronostratigraphic correlations and for interpretation of climate-volcanism interactions. Volcanic events from low-latitude regions are of particular interest as the related sulphate aerosol travelling through the stratosphere can reach the polar sheets forming inter-hemispheric (Greenland and Antarctica) signals preserved in the ice. Within the glaciological record of globally significant volcanic markers, the AD1259 signal represents one of most prominent events over the last thousands years. Its source has been long debated. On the basis of recent field investigations (Lavigne et al., 2013; Vidal et al., 2015), it has been proposed that Mount Samalas on Lombok Island (Indonesia) represents the source responsible for the polar event. With the goal of bringing distal tephrochronological evidence to source identification, we have attempted to identify volcanic ash associated to the AD 1259 sulphate pulse. To this purpose we used firn and ice-core samples from two East Antarctic Plateau sites: Concordia-Dome C (75°06' S, 123°20' E, 3233 m) and Talos Dome (72°49'S, 159°11'E, 2315 m). Our high-resolution studies included sample processing in a Class 100 clean room using established ultra-clean procedures for insoluble microparticle analyses, Coulter counter grain size measurements, scanning electron microscope observations and the geochemical (major elements) composition from the recently set ISTERRE Jeol JXA 8230 Superprobe and calibrated for small particles analysis. Despite the difficulty of studying such minute fragments, within both cores we located and characterised multiple tiny (micron-size) glass shards concomitant with the volcanic peak. We present preliminary results alongside comparison

  3. Dietary branched-chain amino acid (BCAA) and tumor growth

    Energy Technology Data Exchange (ETDEWEB)

    Chan, W.; Baron, L.; Baron, P.; White, F.; Banks, W.L. Jr.

    1986-03-05

    The effects of high dietary BCAA on tumor growth was examined in adult male Fischer 344 rats inoculated with 10/sup 6/ viable MCA fibrosarcoma cells. Ten days after tumor inoculation, when tumors were of palpable size, rats were divided into two groups at random. The experimental(E) group was fed the AIN-76 diet supplemented with 4X the BCAA content of diet casein and the control(C) group was fed the AIN-76 made isonitrogenous with the E diet by glutamic acid supplementation. Five rats from each group were killed at days 0,3,6, and 9. Rats were injected with /sup 14/C-Tyrosine and /sup 3/H-Thymidine i.p. (2 and 4 uCi/100g BW, respectively) an hour before they were killed. The incorporation of /sup 14/C and /sup 3/H into the acid insoluble fraction of the tumor tissues samples were measured. Single cell suspension of tumor were prepared for cell cycle kinetics analysis using a Coulter EPICS IV flow microflorometer. The percentage of normal and hyperdiploid cells were analyzed. Results showed that both tumor size and weight were doubled at each time point the rats were killed. At day 0, the /sup 3/H and /sup 14/C incorporation were 32 +/- 10dpm and 27 +/- 4dpm/mg tumor, respectively. The /sup 3/H incorporation dropped in both diet groups at days 6 and 9 but the /sup 14/C incorporation showed a decrease only at day 9. These changes were statistically significant, P>0.05. No difference in the tumor growth parameters used in this study can be attributed to the high dietary BCAA.

  4. Lymphocytes subsets in children with febrile convulsions.

    Science.gov (United States)

    Tuncer, Oğuz; Karaman, Sait; Caksen, Hüseyin; Oner, Ahmet Faik; Odabas, Dursun; Yilmaz, Cahide; Atas, Bülent

    2007-07-01

    In this study, lymphocytes subsets including blood CD3, CD4, CD8, CD16, CD19, and CD56 values were analyzed in children with febrile convulsion (FC) to determine whether there was the association of lymphocytes subsets in the pathogenesis of FC. The study includes 48 children with FC, and 55 healthy age matched control subjects, followed in Yüzüncü Yil University, Faculty of Medicine, Department of Pediatrics between October 2003 and June 2004. Blood CD3, CD4, CD8, CD16, CD19, and CD56 values were examined in the study and control groups. The analyses were performed in the Hematology Laboratory, Yüzüncü Yil University Faculty of Medicine, with flow cytometer device (Coulter Epics XL2, Flow Cytometer). A total of 48 children [17 girls (35.5%) and 31 boys (64.5%)], aged 6 months to 60 months (mean 22.20 +/- 13.75 months) with FC and 55 healthy children [28 girls (51%) and 27 boys (49%)], aged 6 months to 60 months (mean 28.87 +/- 17.04 months) were included in the study. When compared with the control group, the study found significantly decreased blood CD3 and CD4 values in the study group (p .05). When comparing the children with and without positive family history for FC, the study did not find any difference for all CD values between the groups (p >.05). Similarly, there was not significant difference in CD values between the children with simple and complex FC (p >.05). The findings suggested that decreased blood CD3 and CD4 values might be responsible for the infections connected with FC or that they might be related to the pathogenesis of FC in some children.

  5. THE EFFECTS OF SUBMAXIMAL EXERCISES ON THE THYROID HORMONAL METABOLISM OF SEDENTARY INDIVIDUALS

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    Mustafa AKIL

    2011-04-01

    Full Text Available The objective of this study is to analyze the effects of acute exercises the sedentary individuals do on thyroid hormonal metabolism. The research was carried out on 14 sedentary males between the ages of 18 and 24. The study was approved by the local committee for ethics. The average age of the subjects was: 20,4 ± 0,65 years, their average body weights: 76,86 ± 2,08 kg and their average height was: 175,07 ± 1,26 cm. As for the exercise application, the subjects were made tired through Bruce protocol.10 cc of blood samples were taken from the subjects each time before the exercise, after the exercise, 24 hours after the exercise and 48 hours after the exercise. In the obtained blood samples, the parameters of serum TSH, T3, T4, were analyzed in Backman LX-20 auto analyzer through Lot No:T1142, Mg Lot No:907035, P Lot No:903417 Backman Coulter test kits. The arithmetic averages, and standard deviations of all the parameters were determined. The repeating ANOVA test was used for the determination of the difference between the parameters. Confidence interval was chosen as 95% and the values of p≤0.05 and below was accepted as statistically significant. According to the measurements before and after the exercises, significant differences were found between the parameters of TSH, T3, and T4 (p≤0.05.In conclusion, it can be said that the protocol of these acute submaximal exercises done by sedentary individuals decreased serum TSH, T3, and T4 levels and it approached to normal levels 48 hours after the exercises.

  6. Design and parameter optimization of ditching and compacting parts of rapeseed carpet seedling transplanter%油菜毯状苗移栽机开沟镇压部件设计与参数优化

    Institute of Scientific and Technical Information of China (English)

    吴俊; 汤庆; 袁文胜; 王苏飞; 吴崇友

    2016-01-01

    Nowadays, rapeseed has been considered as the most important oil crop in China, and the rapeseed seedling transplantation has become a main cultivation pattern in the crop rotation of rice and rapeseed. The existing transplanter is still with low working efficiency and not suitable for paddy soil, which has poor fluidity of soil. In aiming of solving these problems, a fully automatic cultivation pattern was proposed, which cut and picked seedling blocks with high speed and then transplanted them under the condition of sticky heavy soil. By using the slicing and transplanting principle of rice transplanter, the functions of ditching, covering and compacting the soil were set up, and finally a rapeseed seedling carpet transplanter was formed, which was suitable for the transplantation in paddy stubble field instead of being limited in paddy field. In view of the original machine’s drawbacks such as little installation space of underframe and light counterbalance weight, the paper described a design of a driven ditching device with oblique ripple and a V-shaped press wheel.In the process of observing the power, the design skillfully use the origin fan pulley on the engine of the transplanter chassis to drive the hydraulic pump. In aim of realizing a continuous adjustment of the running speed and ripple disc coulter of the machine to meet the requirement of different soil conditions, a series of hydraulic valve has been put into use. The designed machine could adapt to different soil condition. By analyzing the characteristics of transplanter and the requirements for transplanting the rapeseed blanket seedlings, the paper accomplished the calculation of the diameter and width of wave coulter. The paper carried out an analysis on motion trail based on the determination of disc parameter, and established the association of principal parameters such as forward speed, depth of ditching and rotation speed of wave coulter. The conclusion was drawn that when the maximum

  7. Evaluation of the Serum Zinc Level in Erosive and Non-Erosive Oral Lichen Planus

    Directory of Open Access Journals (Sweden)

    Gholizadeh N.

    2013-10-01

    Full Text Available Statement of Problem: Lichen planus is a chronic inflammatory immunologic-based disease involving skin and mucosa. This disease is generally divided into two categories: erosive and non-erosive. Many etiologic factors are deliberated regarding the disease; however, the disorders of immune system and the role of cytotoxic T-lymphocytes and monocytes are more highlighted. Zinc is an imperative element for the growth of epithelium and its deficiency induces the cytotoxic activity of T-helper2 cells which seems to be associated with lichen planus. Purpose: This study aimed to evaluate the levels of serum zinc in erosive and non-erosive oral lichen planus (OLP and compares it with the healthy control group to find out any feasible inference. Materials and Method: A total of 22 patients with erosive oral lichen planus, 22 pa-tients with non erosive OLP and 44 healthy individuals as the control group were recruited in this descriptive-comparative study. All the participants were selected from the referees to the department of oral medicine, school of dentistry, Tabriz University of medical sciences. Serum zinc level was examined for all the individuals with liquid-stat kit (Beckman Instruments Inc., Carlsbad, CA. Data were analyzed by adopting the ANOVA and Tukey tests through SPSS 16 statistical software. Results: The mean age of patients with erosive and non-erosive LP was 41.7 and 41.3 years, respectively. The mean age of the healthy control group was 34.4 years .The mean serum zinc levels in the erosive and non erosive lichen planus groups and control groups were 8.3 (1.15, 11.15 (0.92 and 15.74 (1.75 μg/dl respectively. The difference was statistically significant (p< 0.05. Conclusion: The serum zinc levels were decreased in patients with erosive oral lichen planus. This finding may probably indicate the promising role of zinc in development of oral lichen planus.

  8. Establishment of Method for Detecting Red Blood Cell Osmotic Fragility by Flow Cytometry%流式细胞术检测红细胞渗透脆性试验方法的建立

    Institute of Scientific and Technical Information of China (English)

    朱红艳; 孟强; 欧阳红梅; 董婷; 张琼月; 周友全; 平竹仙

    2016-01-01

    目的:建立流式细胞术检测红细胞渗透脆性的方法.方法:将红细胞悬浮于由Nacl液和去离子水制备的不同浓度低渗盐溶液(0.70 ml生理盐水+0.3 ml去离子水,0.60 ml生理盐水+0.40 ml去离子水和0.55 ml生理盐水+0.45 ml去离子水)中,利用流式细胞仪及时测定残留红细胞数量.结果:3种NaCl稀释浓度中,流式细胞仪检测的不同时间区域剩余红细胞数量百分比差别不大.选取B+C+D+E+F+G区域比较,不同NaCl稀释浓度下3组的剩余红细胞数量百分比均有差异;正常对照组的剩余红细胞百分比显著低于血红蛋白病组;遗传性球形红细胞增多症(hereditary spherocytosis,HS)组显著低于血红蛋白病组和正常组(P<0.05).3种不同稀释浓度比较发现,第2种浓度(0.60 ml N.S.+0.40 ml H2O)更适合于本实验室Beckman-Coulter流式细胞仪FC500对遗传性球形红细胞增多症的筛查.结论:选用流式细胞术检测红细胞渗透脆性简单、快速、客观、经济.本方法可能是一种非常有效的对遗传性球形红细胞进行初筛的方法.

  9. A high-temperature catalytic oxidation method for the determination of dissolved organic carbon in seawater: analysis and improvement

    Science.gov (United States)

    Suzuki, Yoshimi; Tanoue, Eiichiro; Ito, Hiroshi

    1992-02-01

    The high-temperature catalytic oxidation (HTCO) method for the determination of dissolved organic carbon in seawater, reported by SUGIMURA and SUZUKI (1988, Marine Chemistry, 24, 105-131) has been improved to provide more valid data on the levels of dissolved organic carbon (DOC). The improvements are as follows. (1) An open-close valve, made from an alloy of iron, molybdenum, nickel and chromium and without a silicone septum, is used as the injection port. (2) Copper oxide and sulfix (mixture of AgO and CoO) are packed with a 3% platinum (Pt) catalyst into the combustion tube for the complete elimination of halogenated and sulfur compounds. (3) The top of the catalyst is covered with Pt gauze to prevent the Pt catalyst from flying upwards. (4) A glass water-trap, containing phosphoric acid and silver nitrate in solution, is connected just below the outlet of the combustion tube. (5) A high-sensitivity, infra-red gas analyser (Beckman model 880) is used. The blank value for the system is checked using water prepared by passage through the improved HTCO system. The total blank value (system blank plus water blank) ranges from 15 to 30 μM C. Of the total blank volume, the blank value for the system which was examined using water collected from the HTCO system, was less than 3 μM C. The greatest contributions to the blank value in this machine are made by the organic matter originally present in deionized water. The oxidation efficiency of the catalyst with different levels of Pt was examined for measurements of DOC. Although there is little difference in detected concentrations of DOC from the surface to deep waters with levels of 1.5, 3 and 5% Pt in the catalyst, the alumina impregnated with higher levels of Pt is much better for the complete oxidation of the DOC in seawater. The size of the combustion tube, the quality of the catalyst and the uniform distribution of the furnace temperature are key elements in determining the oxidative capacity of the system. It

  10. 不同检测系统尿微量清蛋白测定结果的可比性及偏倚评估

    Institute of Scientific and Technical Information of China (English)

    黄舒; 熊海燕; 洪煜婧

    2016-01-01

    目的 对两种不同检测系统尿微量清蛋白测定结果进行方法比对和偏倚评估 ,探讨其可比性.方法 参照美国临床和实验室标准化协会(CLSI)EP9-A2文件要求 ,以BeckmanIMMAGE800全自动免疫分析仪作为比对方法(X) ,日立7600全自动生化分析仪为实验方法(Y) ,用患者新鲜尿液分别用上述不同检测系统同时检测微量清蛋白 ,计算实验方法(Y)和比对方法(X)之间的相对偏差(SE% ) ,以卫生部临检中心室间质评允许误差(Ea)范围的1/2为判断标准 ,评价各系统测定结果的可比性.结果 实验系统与比较系统间具有良好的相关性(r=0 .998);比较方法检测结果为(80 .8 ± 55 .2)mg/L ,实验方法为(84 .9 ± 57 .7) mg/L ,经配对设计的 t检验 ,差异有统计学意义(t=9 .504 ,P<0 .01) ,实验方法明显高于比较方法 ;但以临床可接受性能判断标准(SE% <1/2 CLIA′88 TEa) ,认为实验系统的检测结果可接受.结论 上述不同检测系统检测结果具有较好的相关性 ,临床可接受性能评价具有可比性.经过方法对比及偏差评估确定在该实验室使用生化仪替代免疫仪检测是可行的.

  11. 希森美康 XE -2100和贝克曼 LH750血细胞分析仪检测结果比对分析

    Institute of Scientific and Technical Information of China (English)

    朱爱萍; 梁树芬; 闫彩香; 郭存九

    2016-01-01

    目的:探讨希森美康(sysmex)XE -2100和贝壳曼(Beckman)LH750血细胞分析仪检测结果的可比性。方法:按照美国临床和实验室标准化协会(NCCLS)EP9- A2文件要求,检测乙二胺四乙酸二钾(EDTA - K2)抗凝新鲜静脉全血标本,以 XE -2100为参考仪器、Beckman LH750为比对仪器,分析两台仪器白细胞计数( WBC)、红细胞(RBC)、血红蛋白(Hb)、血细胞比容(HCT)和血小板(PLT)检测结果的相关性;在各个医学决定水平(Xc)处,计算两台仪器之间结果的偏倚,以美国临床实验室修正法案(1988)(CLIA′88)规定的允许总误差(TEa)的1/2作为评价标准,两台仪器 WBC,RBC,Hb,HCT,PLT 比对的可接受范围依次为±7.5%,±3.0%,±3.5%,±3.0%,±12.5%。结果:两台仪器 WBC,RBC,Hb,HCT 和 PLT 检测结果呈正相关,相关系数分别为0.994,0.993,0.999,0.996,0.998。在各个医学决定水平处,两台仪器间测定结果的偏倚均在判断标准规定的范围内。结论:两台血细胞分析仪5项指标检测结果具有较好的一致性,预期偏倚可接受。

  12. Excreção urinária de 17-Cetoesteroides neutros no cavalo normal e no cavalo castrado

    Directory of Open Access Journals (Sweden)

    Fernando Ubatuba

    1954-06-01

    Full Text Available Total urinary neutral 17-steroids were determined in normal and in castrated horses. One liter of a 15-26 hours urine collection was hydrolysed by refluxing with 10% HC1 (v/v for ten minutes and extracted with peroxyde-free ethyl ether. The extract was purified by washing with saturated NaHCO³ and KOH solutions. One half of the crude neutral fraction was fractionated with Girard's "T" reagent . The Zimmermann reaction was performed both in the ketonic and in the crude neutral extracts, using alcoholic 2.5N KOH and a 60 minutes period for the colour development in the dark. Optical density measuments were made in a grating Coleman Universal Spectrophotometer at 420 mµ and 520mµ; for the crude neutral fraction a colour correction equation was applied. The aliquot fraction used for colorimety was adjusted for keeping optical density measurements within the range 0.2 to 0.7. Androsterone (mp. 184-184.5°C with an absorption maximum at 290.5 mµ (Beckman Model DU Spectrophotometer was used as a reference standard. Table I, ilustrates the results obtained. At the 0.05 probability level there is a significant difference among castrated and normal group means (Fischer's "t" test. when were used the data obtained from the ketonic fractions; in spite of the use of a colour correction applied for inespecific chromogens, the same results could not be obtained with the crude neutral fractions, Since Girard's reagent fractionation is generaly accepted as the best method for correcting the inespecific chromogen interference in the determination of the 17-ketosteroids by the Zimmermann reaction, we emphasize the value of the results obtained with the ketonic fractions. From these results it appears, as occurs in others mammals, that castrated horses show a lower level of urinary 17-ketosteroids excretion than the normal horses. The significance of the horse testis contribution for the neutral urinary steroid metabolites is discussed. Since horse urine has a

  13. Comparison of microemulsion electrokinetic chromatography with high-performance liquid chromatography for fingerprint analysis of resina draconis.

    Science.gov (United States)

    Cao, Yuhua; Gong, Wenjun; Li, Nan; Yin, Changna; Wang, Yun

    2008-11-01

    Microemulsion electrokinetic chromatography (MEEKC) has been developed for fingerprint analysis of resina draconis, a substitute for sanguis draconis in the Chinese market. The microemulsion as the running buffer was made up of 3.3% (w/v) sodium dodecyl sulfate (SDS), 6.6% (w/v) n-butanol, 0.8% (w/v) n-octane, and 10 mmol/L sodium tetraborate buffer (pH 9.2), which was also used as the solvent for ultrasonic extraction of both water- and fat-soluble compounds in the traditional Chinese medicine samples. Four batches of resina draconis obtained from different pharmaceutical factories located in different geographic regions were used to establish the electrophoretic fingerprint. MEEKC was performed using a Beckman PACE/MDQ system equipped with a diode-array detector and with monitoring at 280 nm. The fingerprint of resina draconis comprised 27 common peaks within 100 min. The relative standard deviations of the relative migration time of these common peaks were less than 2.1%. Through repetitive injection of the sample solution six times in 24 h, all relative standard deviations of the migration time and peak area of loureirin A and loureirin B were less than 2.5 and 3.8%, which demonstrated that the method had good stability and reproducibility. The relative peak areas of these common peaks in the electropherograms of four batches of resina draconis were processed with two mathematical methods, the correlation coefficient and the interangle cosine, to valuate the similarity. The values of the similarity degree of all samples were more than 0.91, which showed resina draconis samples from different origins were consistent. On the other hand, high-performance liquid chromatography (HPLC) coupled with photodiode-array detection was also applied to establish the fingerprint of resina draconis. The samples were separated with a LiChrospher C(18) column using acetonitrile (solvent A) and water containing 0.1% H(3)PO(4) (solvent B) as the mobile phase in linear gradient

  14. Polymorphisms of some cytokines and chronic hepatitis B and C virus infection

    Institute of Scientific and Technical Information of China (English)

    Qiu-Ju Gao; Dian-Wu Liu; Shi-Yong Zhang; Min Jia; Li-Min Wang; Li-Hong Wu; Shu-Yun Wang; Li-Xin Tong

    2009-01-01

    AIM: To study the relationship between the polymorphismsin some cytokines and the outcome of hepatitis B virus (HBV) and hepatitis C virus (HCV) infection.METHODS: Samples were obtained from 203 patients infected with HBV and/or HCV while donating plasma in 1987, and 74 controls were obtained from a rural area of North China. Antibodies to HBV or HCV antigens were detected by enzyme-linked immunoassay. The presence of viral particles in the serum was determined by nested reverse-transcriptase polymerase chain reaction (PCR). Hepatocellular injury, as revealed by alanine aminotransferase (ALT) and aspartate aminotransferase level, was detected by a Beckman LX-20 analyzer.DNA was extracted from blood cells. Then, the single nucleotide polymorphisms of IL-2-330, IFN-γ+874,IL-10-1082/-592 and IL-4-589 were investigated by restriction fragment length polymorphism-PCR or sequence specific primer-PCR.RESULTS: Persistent infection with HBV, HCV, and HBV/HCV coinfection was associated with IL-2-330 TT genotype and T allele, IFN-γ+874 AA genotype, and IL-10-1082 AA genotype. The clinical outcome of HBV and/or HCV infection was associated with IL-2-330 TT genotype and T allele, IFN-γ+874 AA genotype,and IL-10-1082 AA genotype. IL-2-330 GG genotype frequency showed a negative correlation with clinical progression, IL-10-1082 AA genotype frequency showed a positive correlation and IL-10-1082 AG genotype frequency showed a negative correlation with clinical progression. HCV RNA positive expression was associated with IL-10-1082 AA genotype and the A allele frequency. Abnormal serum ALT level was associated with IL-10-592 AC genotype frequency and IL-4-589 CC genotype, CT genotype, and the C allele.CONCLUSION: These results suggest that polymorphismsin some cytokine genes influence persistent HBV and HCV infection, clinical outcome, HCV replication,and liver damage.

  15. Generation of Microcellular Biodegradable Polycaprolactone Foams in Supercritical Carbon Dioxide

    Institute of Scientific and Technical Information of China (English)

    Xu Qun; Ren Xian-wen; Chang Yu-ning; Yu Long; Wang Jing-wu

    2004-01-01

    Present now the application of microcellular polymeric materials in biomedical field is growing rapidly, as that of guided tissue regeneration and cell transplantation. As far as guided tissue regeneration is concerned, porous implants are used as size selective membrane to promote the growth of a special tissue in a healing site. Ideally, the implant should be inherently biocompatible,have well-defined cell size and be resorbable with appropriate biodegradation rates.Poly(a-caprolactone) (PCL) is a kind of materials suit for the demands above. PCL is biocompatible and biodegradable aliphatic polyester which is nontoxic for living organisms and bioresorbable after a period of implantation. Because of its unique combination of biocompatibility, permeability and biodegradability, PCL and some of its copolymer with lactides and glycolide have been widely applied in medicine as artificial skin, artificial bone and containers for sustained drug release.Goel and Beckman have reported a new method to generate microcellular poly(methy l methacrylate) foams in which the samples are saturated with CO2 under a series of supercritical (SC)conditions, and then the system is rapidly depressurized to atmospheric pressure at constant temperature. Unlike traditional methods, it reduces glass-transition temperature (Tg) of the mixture to below the experimental temperature rather than directly heat the system above Tg. In this process of nucleation, no phase separation occurs as well as no phase boundary meets, so the cellular structure of the foam can be retained better.In this work, we have generated PCL foams by using supercritical CO2. Because of the low glass transition temperature (Tg = -60 ℃) of PCL far below the ice point, the experimental temperature in our study is much higher than Tg, which is different from the studies by others before. A series of variable factors on the foam structure as saturation temperature, saturation pressure, saturation time and depressurization

  16. High performance capillary electrophoresis analysis of polypeptide in protein hydrolysis ingredients of brain tissue%脑蛋白水解物中多肽组分的高效毛细管电泳分析

    Institute of Scientific and Technical Information of China (English)

    王辰; 黄慧玲; 莫丽冬; 范维佳; 阎维维

    2012-01-01

    目的 利用高效毛细管电泳法比较2种水解方法提纯的脑蛋白水解物中的多肽组分,同时监测进口脑蛋白水解物注射液的多肽组分分布.方法 采用固相萃取法提取脑组织中的多肽成分,以PACE/A5500型高效毛细管电泳仪对进口脑蛋白水解物注射液和本实验室提取的脑组织匀浆物进行比较.结果 进口脑蛋白水解物注射液中多肽成分大部分集中在>14400区域,本实验室提纯的脑蛋白水解物成分中多肽多集中在相对分子质量6000 ~ 14 400区域,且纯度及含量均较高.结论 高效毛细管电泳法可有效地对脑蛋白水解物注射液中多肽组分进行分析和比较,2种提纯方法对脑蛋白的裂解能力相差不大.%Objective To extract the protein hydrolysis ingredients of brain tissue were extracted and to analyze the components of polypeptide in cerebrolysin by using the method of high-performance capillary electrophoresis (HPCE).Methods Brain homogenate were extracted by solid phase extraction method.Cerebrolysin and brain tissue hydrolyzated extracted in lab were detected by HPCE (PACE/A5500,Beckman).Result The result of HPCE showed that the cerebrolysin injections molecular weight of most polypeptides were more than 14 400.The purity and content of cerebrolysin was better and the molecular weights were mainly between 6000 and 14 400.Conclusion The method of HPCE can be effectively used in analyzing components of polypeptides of brain tissue hydrolyzated.There is no difference of protein hydrolysis ingredients of brain tissue extracted by 2 kinds of extraction methods.

  17. Internal quality control system for non-stationary, non-ergodic analytical processes based upon exponentially weighted estimation of process means and process standard deviation.

    Science.gov (United States)

    Jansen, Rob T P; Laeven, Mark; Kardol, Wim

    2002-06-01

    The analytical processes in clinical laboratories should be considered to be non-stationary, non-ergodic and probably non-stochastic processes. Both the process mean and the process standard deviation vary. The variation can be different at different levels of concentration. This behavior is shown in five examples of different analytical systems: alkaline phosphatase on the Hitachi 911 analyzer (Roche), vitamin B12 on the Access analyzer (Beckman), prothrombin time and activated partial thromboplastin time on the STA Compact analyzer (Roche) and PO2 on the ABL 520 analyzer (Radiometer). A model is proposed to assess the status of a process. An exponentially weighted moving average and standard deviation was used to estimate process mean and standard deviation. Process means were estimated overall and for each control level. The process standard deviation was estimated in terms of within-run standard deviation. Limits were defined in accordance with state of the art- or biological variance-derived cut-offs. The examples given are real, not simulated, data. Individual control sample results were normalized to a target value and target standard deviation. The normalized values were used in the exponentially weighted algorithm. The weighting factor was based on a process time constant, which was estimated from the period between two calibration or maintenance procedures. The proposed system was compared with Westgard rules. The Westgard rules perform well, despite the underlying presumption of ergodicity. This is mainly caused by the introduction of the starting rule of 12s, which proves essential to prevent a large number of rule violations. The probability of reporting a test result with an analytical error that exceeds the total allowable error was calculated for the proposed system as well as for the Westgard rules. The proposed method performed better. The proposed algorithm was implemented in a computer program running on computers to which the analyzers were

  18. Capillary electrophoresis for the assay of fixed-dose combination tablets of artesunate and amodiaquine

    Directory of Open Access Journals (Sweden)

    Amin N’Cho

    2012-05-01

    Full Text Available Abstract Background Quality control of drugs in formulations is still a major challenge in developing countries. For the quality control of artesunate and amodiaquine tablets in fixed-dose combination, only liquid chromatographic methods have been proposed in the literature. There are no capillary electrophoretic methods reported for the determination of these active substances, although this technique presents several advantages over liquid chromatography (long lifetime, low price of the capillary, low volumes of electrolyte consumption in addition to simplicity. In this paper, a reliable capillary electrophoresis method has been developed and validated for the quality control of these drugs in commercial fixed-dose combination tablets. Methods Artesunate and amodiaquine hydrochloride in bilayer tablets were determined by micellar electrokinetic capillary chromatography (MEKC. Analytes were extracted from tablets by sonication with a solvent mixture phosphate buffer pH 7.0-acetonitrile containing benzoic acid as internal standard. Separation was carried out on Beckman capillary electrophoresis system equipped with fused silica capillary, 30 cm long (20 cm to detector × 50 μm internal diameter, using a 25 mM borate buffer pH 9.2 containing 30 mM sodium dodecyl sulfate as background electrolyte, a 500 V cm−1 electric field and a detection wavelength of 214 nm. Results Artesunate, amodiaquine and benzoic acid were separated in 6 min. The method was found to be reliable with respect to specificity,linearity of the calibration line (r2 > 0.995, recovery from synthetic tablets (in the range 98–102%, repeatability (RSD 2–3%, n = 7 analytical procedures. Application to four batches of commercial formulations with different dosages gave content in good agreement with the declared content. Conclusion The MEKC method proposed is reliable for the determination of artesunate and amodiaquine hydrochloride in fixed

  19. High-performance liquid chromatographic method for the simultaneous detection of malonaldehyde, acetaldehyde, formaldehyde, acetone and propionaldehyde to monitor the oxidative stress in heart.

    Science.gov (United States)

    Cordis, G A; Bagchi, D; Maulik, N; Das, D K

    1994-02-11

    Lipid peroxidation (LPO) is the oxidative deterioration of polyunsaturated fatty acids (PUFA) with the production of lipid hydroperoxides, cyclic peroxides, cyclic endoperoxides, and finally fragmentation to ketones and aldehydes (including malonaldehyde, MDA). Estimation of LPO through MDA formation measured by assaying thiobarbituric acid (TBA) reactive products remains the method of choice to study the development of oxidative stress in tissues. However, MDA estimation by TBA reactive products is non-specific and often gives erroneous results. In this report we describe a method using high-performance liquid chromatographic separation to estimate MDA, formaldehyde (FDA), acetaldehyde (ADA), acetone, and propionaldehyde (PDA), the degradation products of oxygen-derived free radicals (ODFR) and PUFA, as presumptive markers for LPO. Oxidative stress was induced in the tissue by perfusing an isolated rat heart with hydroxyl radical generating system (xanthine + xanthine oxidase + FeCl3 + EDTA). The coronary effluents were collected, derivatized with 2,4-dinitrophenylhydrazine (DNPH), and extracted with pentane. Aliquots of 25 microliters in acetonitrile were injected onto a Beckman Ultrasphere C18 (3 microns) column. The products were eluted isocratically with a mobile phase containing acetonitrile-water-acetic acid (40:60:0.1, v/v/v), measured at three different wavelengths (307, 325 and 356 nm) using a Waters M-490 multichannel UV detector and collected for gas chromatography-mass spectrometry (GC-MS) analysis. The peaks were identified by cochromatography with DNPH derivatives of authentic standards, peak addition, UV pattern of absorption at the three wavelengths, and by GC-MS. The retention items of MDA, FDA, ADA, acetone, and PDA were 5.3, 6.6, 10.3, 16.5, and 20.5 min, respectively. The results of our study indicated progressive increase of all five lipid metabolites as a function of the duration of ODFR perfusion. Hydroxyl radical scavengers, superoxide

  20. The effect of steam sterilization on the accuracy of spring-style mechanical torque devices for dental implants

    Directory of Open Access Journals (Sweden)

    Mahshid M

    2012-07-01

    Full Text Available Minoo Mahshid,1 Aboulfazl Saboury,1 Ali Fayaz,1 Seyed Jalil Sadr,1 Friedrich Lampert,2 Maziar Mir2,31Department of Prosthodontics, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2Department of Conservative Dentistry, Aachen RWTH Hospital, Aachen, Germany; 3Beckman Laser Institute, UCI, Irvine, CA, USABackground: Mechanical torque devices (MTDs are one of the most commonly recommended devices used to deliver optimal torque to the screw of dental implants. Recently, high variability has been reported about the accuracy of spring-style mechanical torque devices (S-S MTDs. Joint stability and survival rate of fixed implant supported prosthesis depends on the accuracy of these devices. Currently, there is limited information on the steam sterilization influence on the accuracy of MTDs. The purpose of this study was to assess the effect of steam sterilization on the accuracy (±10% of the target torque of spring-style mechanical torque devices for dental implants.Materials and methods: Fifteen new S-S MTDs and their appropriate drivers from three different manufacturers (Nobel Biocare, Straumann [ITI], and Biomet 3i [3i] were selected. Peak torque of devices (5 in each subgroup was measured before and after autoclaving using a Tohnichi torque gauge. Descriptive statistical analysis was used and a repeated-measures ANOVA with type of device as a between-subject comparison was performed to assess the difference in accuracy among the three groups of spring-style mechanical torque devices after sterilization. A Bonferroni post hoc test was used to assess pairwise comparisons.Results: Before steam sterilization, all the tested devices stayed within 10% of their target values. After 100 sterilization cycles, results didn't show any significant difference between raw and absolute error values in the Nobel Biocare and ITI devices; however the results demonstrated an increase of error values in the 3i group (P < 0.05. Raw error

  1. A entoação das sentenças clivadas em português brasileiro e a interface sintaxe-fonologia

    Directory of Open Access Journals (Sweden)

    Flaviane Romani Fernandes-Svartman

    2012-06-01

    Full Text Available Este trabalho, desenvolvido no quadro teórico da Fonologia Entoacional (cf. Pierrehumbert,1980; Beckman and Pierrehumbert, 1986; Ladd 1996; Jun, 2005, visa ao estudo comparativoda estrutura entoacional das sentenças neutras e clivadas (sentenças clivadas, clivadasinvertidas e clivadas invertidas reduzidas do português brasileiro (doravante, PB. Conformeos resultados obtidos por Frota (1994, Vigário (1998 e Fernandes-Svartman (2007c, em estruturassintáticas especiais do português europeu, o contorno entoacional sofre alterações locaise, algumas vezes, globais, se comparado ao contorno das sentenças neutras. Nossa hipótese éde que também em PB ocorram alterações no contorno entoacional de estruturas sintáticasespeciais, como no caso das sentenças clivadas. Os resultados alcançados por este trabalhoconfirmam tal hipótese, na medida em que foram encontradas diferenças significativas entreo contorno entoacional das sentenças neutras e o contorno entoacional das sentenças clivadasdo PB. Enquanto para as sentenças neutras foram encontrados acentos tonais associados a,praticamente, cada palavra prosódica da sentença e ausência de acentos frasais associados afronteiras de frases fonológicas, para as sentenças clivadas, foram encontrados: (i acento tonalassociado obrigatoriamente à palavra prosódica cabeça da frase fonológica na qual o sujeitofocalizado é mapeado; (ii acento frasal associado opcionalmente à fronteira direita dessa mesmafrase fonológica; e (iii ausência de acentos tonais associados a palavras prosódicas intermediárias(entre a palavra prosódica cabeça da frase fonológica na qual o sujeito focalizado é mapeado ea palavra prosódica cabeça da última frase fonológica da frase entoacional. No caso das sentençasclivadas, a presença de acento frasal associado à fronteira direita da frase fonológica na qual o sujeito focalizado é mapeado pode, por hipótese, estar relacionada à codificação de

  2. The multi-xenobiotic resistance (MXR) efflux activity in hemocytes of Mytilus edulis is mediated by an ATP binding cassette transporter of class C (ABCC) principally inducible in eosinophilic granulocytes.

    Science.gov (United States)

    Rioult, Damien; Pasquier, Jennifer; Boulangé-Lecomte, Céline; Poret, Agnès; Abbas, Imane; Marin, Matthieu; Minier, Christophe; Le Foll, Frank

    2014-08-01

    In marine and estuarine species, immunotoxic and/or immunomodulatory mechanisms are the crossroad of interactions between xenobiotics, microorganisms and physicochemical variations of the environment. In mussels, immunity relies exclusively on innate responses carried out by cells collectively called hemocytes and found in the open hemolymphatic circulatory system of these organisms. However, hemocytes do not form a homogenous population of immune cells since distinct subtypes of mussel blood cells can be distinguished by cytochemistry, flow cytometry or cell motility analysis. Previous studies have also shown that these cells are able to efflux xenobiotics by means of ATP binding cassette (ABC) transporter activities conferring a multixenobiotic resistance (MXR) phenotype. ABC transporters corresponding to vertebrate class B/P-glycoprotein (P-gp) and to class C/multidrug resistance related protein (MRP) are characterized in Mytilidae. Herein, we have investigated the relative contributions of ABCB- and ABCC-mediated efflux within the different hemocyte subpopulations of Mytilus edulis mussels, collected from areas differentially impacted by chemical contaminants in Normandy (France). RT-PCR analyses provide evidence for the presence of ABCB and ABCC transporters transcripts in hemocytes. Immunodetection of ABCB/P-gp with the monoclonal antibody UIC2 in living hemocytes revealed that expression was restricted to granular structures of spread cells. Efflux transporter activities, with calcein-AM as fluorescent probe, were measured by combining flow cytometry to accurate Coulter cell size measurements in order to get a cell-volume normalized fluorescence concentration. In these conditions, basal fluorescence levels were higher in hemocytes originating from Yport (control site) than in cells collected from the harbor of Le Havre, where mussels are more exposed to with persistent pollutants. By using specific ABCB/P-gp (verapamil, PSC833, zosuquidar) and ABCC/MRP (MK

  3. Cell type-dependent uptake, localization, and cytotoxicity of 1.9 nm gold nanoparticles

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    Coulter JA

    2012-06-01

    Full Text Available Jonathan A Coulter,1 Suneil Jain,2 Karl T Butterworth,2 Laura Taggart,2 Glenn Dickson,2 Stephen J McMahon,3 Wendy Hyland,1 Mark F Muir,3 Coleman Trainor,2 Alan Hounsell,2,4 Joe M O'Sullivan,2,4 Giuseppe Schettino,2 Fred Currell,3 David G Hirst,1 Kevin M Prise21School of Pharmacy, McClay Research Centre, 2Centre for Cancer Research and Cell Biology, 3School of Mathematics and Physics, Queens University Belfast, 4Belfast Health and Social Care Trust, Belfast, IrelandBackground: This follow-up study aims to determine the physical parameters which govern the differential radiosensitization capacity of two tumor cell lines and one immortalized normal cell line to 1.9 nm gold nanoparticles. In addition to comparing the uptake potential, localization, and cytotoxicity of 1.9 nm gold nanoparticles, the current study also draws on comparisons between nanoparticle size and total nanoparticle uptake based on previously published data.Methods: We quantified gold nanoparticle uptake using atomic emission spectroscopy and imaged intracellular localization by transmission electron microscopy. Cell growth delay and clonogenic assays were used to determine cytotoxicity and radiosensitization potential, respectively. Mechanistic data were obtained by Western blot, flow cytometry, and assays for reactive oxygen species.Results: Gold nanoparticle uptake was preferentially observed in tumor cells, resulting in an increased expression of cleaved caspase proteins and an accumulation of cells in sub G1 phase. Despite this, gold nanoparticle cytotoxicity remained low, with immortalized normal cells exhibiting an LD50 concentration approximately 14 times higher than tumor cells. The surviving fraction for gold nanoparticle-treated cells at 3 Gy compared with that of untreated control cells indicated a strong dependence on cell type in respect to radiosensitization potential.Conclusion: Gold nanoparticles were most avidly endocytosed and localized within cytoplasmic

  4. Structural variation of solid core and thickness of porous shell of 1.7 μm core-shell silica particles on chromatographic performance: narrow bore columns.

    Science.gov (United States)

    Omamogho, Jesse O; Hanrahan, John P; Tobin, Joe; Glennon, Jeremy D

    2011-04-15

    Chromatographic and mass transfer kinetic properties of three narrow bore columns (2.1×50 mm) packed with new core-shell 1.7 μm EIROSHELL™-C(18) (EiS-C(18)) particles have been studied. The particles in each column varied in the solid-core to shell particle size ratio (ρ), of 0.59, 0.71 and 0.82, with a porous silica shell thickness of 350, 250 and 150 nm respectively. Scanning and transmission electron microscopy (SEM and TEM), Coulter counter analysis, gas pycnometry, nitrogen sorption analysis and inverse size exclusion chromatography (ISEC) elucidated the physical properties of these materials. The porosity measurement of the packed HILIC and C(18) modified phases provided the means to estimate the phase ratios of the three different shell columns (EiS-150-C(18), EiS-250-C(18) and EiS-350-C(18)). The dependence of the chromatographic performance to the volume fraction of the porous shell was observed for all three columns. The naphtho[2,3-a]pyrene retention factor of k'∼10 on the three EiS-C(18s) employed to obtain the height equivalents to theoretical plates (HETPs) data were achieved by varying the mobile phase compositions and applying the Wilke and Chang relationship to obtain a parallel reduced linear velocity. The Knox fit model gave the coefficient of the reduce HETPs for the three EiS-C(18s). The reduced plate height minimum h(min)=1.9 was achieved for the EiS-150-C(18) column, and generated an efficiency of over 350,000 N/m and h(min)=2.5 equivalent to an efficiency of 200,000 N/m for the EiS-350-C(18) column. The efficiency loss of the EiS-C18 column emanating from the system extra-column volume was discussed with respect to the porous shell thickness.

  5. Proceedings of Patient Reported Outcome Measure’s (PROMs Conference Sheffield 2016: advances in patient reported outcomes research

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    Tim Croudace

    2016-10-01

    longitudinal parameter shifts in epidemiological data: measurement invariance and response shifts in cohort and survey data describing the UK’s Quality of Life Jan R. Boehnke O12 Patient-reported outcomes within health technology decision making: current status and implications for future policy Andrew Trigg, Ruth Howells O13 Can social care needs and well-being be explained by the EQ-5D? Analysis of Health Survey for England dataset Jeshika Singh, Subhash Pokhrel, Louise Longworth O14 Where patients and policy meet: exploring individual-level use of the Long-Term Conditions Questionnaire (LTCQ Caroline Potter, Cheryl Hunter, Laura Kelly, Elizabeth Gibbons, Julian Forder, Angela Coulter, Ray Fitzpatrick, Michele Peters

  6. Determination of reference ranges for full blood count parameters in neonatal cord plasma in Hilla, Babil, Iraq

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    Al-Marzoki JM

    2012-10-01

    Full Text Available Jasim M Al-Marzoki1, Zainab W Al-Maaroof2, Ali H Kadhum31Department of Pediatrics, 2Department of Pathology, Babylon Medical College, 3Babylon Gynecology and Pediatric Teaching Hospital, Hilla, IraqBackground: The health of an individual is known to vary in different countries, in the same country at different times, and in the same individuals at different ages. This means that the condition of individuals must be related to or compared with reference data. Determination of a reference range for the healthy term newborn is clinically important in terms of various complete blood count parameters. The purpose of this study was to establish a local reference range for full blood count parameters in neonatal cord plasma in Hilla, Babil, Iraq.Methods: A total of 220 mothers and their neonates were enrolled in this cross-sectional study from February 2011 to January 2012. Maternal inclusion criteria were age 15–45 years, an uneventful pregnancy, and hemoglobin ≥ 10 g. Neonatal inclusion criteria were full term (37–42 weeks and normal birth weight. The umbilical cord was immediately clamped after delivery of the baby; 3 mL of cord blood was then taken from the umbilical vein and collected in a tube containing ethylenediamine tetra-acetic acid, its plasma was analyzed for full blood count parameters by standard Coulter gram, and the differential leukocyte count was done manually.Results: Mean neonatal hemoglobin was 13.88 ± 1.34 (range 11–17.3 g/dL and mean white cell count was 10.12 ± 2.8 (range 3.1–21.6 × 109/L. Mean platelet count was 267.63 ± 60.62 (range 152–472 × 109/L. No significant differences in red cell, white cell, or platelet counts were found between males and females, except for neutrophil count. The current study shows lower levels of hemoglobin, white cells, and red cells compared with other studies, and there is agreement with some studies and disagreement with others concerning platelet count.Conclusion: Most results

  7. Does chronic alcohol use by HIV-infected patients on d4T/3TC/NVP drug regimen effect the HIV viral load and what is the therapeutic window of the drugs, CD4+ count and WBC count in patients with high viral load during the 9 months period of follow up?

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    Godfrey S. Bbosa

    2013-10-01

    Full Text Available The study investigated the effects of chronic alcohol use on HIV viral load in HIV-infected patients on d4T/3TC/NVP drug regimen during 9 months follow up period. It also determined plasma drug concentrations of d4T, 3TC and NVP; CD4+ and WBC counts for patients with high HIV viral load. A case-control study using repeated measures with serial measurements was used. A total of 41 patients (20 alcohol group and 21 control group were screened for alcohol use using WHO AUDIT tool and chronic alcohol use biomarkers. Blood sampling was done at 3 month intervals for a period of 9 months. HIV viral load was determined using Roche Amplicor HIV-1 monitor test, version 1.5 (Amplicor. The d4T, 3TC and NVP concentrations were determined by Shimadzu Class-VPTM HPLC Chromatography data system version 6.1. The CD4+ cell count was determined using FACSCalibur flow cytometer. The WBC was determined using automated hematological Coulter CBC-5 Hematology Analyzer system. Results show that % patients with HIV viral load ≥400 copies/ml in control group was highest (23.8%, n=5 at 3 month while in chronic alcohol use group, it was at 0 month (35%, n=7 for both WHO AUDIT tool and chronic alcohol-use biomarkers groups. Generally patients with high viral load ≥400 copies/ml was observed in chronic alcohol use as compared to control group in both WHO AUDIT tool and biomarkers group despite of patients having high steady state d4T, 3TC and NVP plasma drug concentrations in circulation that is available to suppress HIV virus. The high viral load could be associated with the emergence of resistance of the HIV virus and these patients generally had a low CD4+ cell count. Some of these patients had no detectable d4T plasma drug concentrations in circulation and most of them with high viral load had sub-therapeutic NVP plasma drug concentrations in their blood circulation. Chronic ethanol use by HIV-infected patients on d4T/3TC/NVP drug regimen increased HIV viral load and

  8. Cytometric evaluation of intracellular IFN-γ and IL-4 levels in thyroid follicular cells from patients with autoimmune thyroid diseases

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    Bossowski Artur

    2011-09-01

    Full Text Available Abstract Background In recent few years is underlined that altered balance of pro- and anti-inflammatory cytokines play an important role in the pathogenesis of AITD. The aim of this study was to estimate intracellular INF-γ and IL-4 levels in thyroid-infiltrating lymphocytes and thyrocytes isolated from thyroid tissues in 54 adolescent patients aged 8-21 years, with Graves' disease (GD; n = 18, Hashimoto's thyroiditis (HT; n = 18 and non-toxic multinodular goiter (NTMG; n = 18. Methods Fresh thyroid tissues were taken on culture medium RPMI -1640, it was mechanically prepared. In next step were added cell activators -12- myristate 13- the acetate (PMA and Ionomycin as well as the inhibitor of transportation of proteins - Breferdin A. They were cultured 24 hours in 50 ml flasks at 37°C in a 5-95% CO2-air water-saturated atmosphere. After that, thyrocytes were identified by mouse mAb directed against human TPO epitope 64 conjugated with rabbit anti-mouse antibodies IgG (Fab'2 labeled by FITC. After incubation at room temperature to each of samples added reagent A fixative the cellular membrane. In next step into the cell suspensions were added reagent B to permeabilization of cellular membrane and specific anti-IL-4-PE or anti-IFN-γ-PE mAbs. Identification of intracellular cytokines in T lymphocytes was performed in the same procedure with application of anti-CD4-PerCP and anti-CD8-PerCP mAbs specific for T lymphocytes. The cells were analyzed in a flow cytometry (Coulter EPICS XL. Results In examined group of patients with GD we observed statistically significant higher mean percentage of cells with phenotype CD4+IL-4 (p Conclusions We conclude that human thyrocytes in autoimmune thyroid disorders could be a source of cytokine production and that their activation influences local interaction with T lymphocytes inflowing to the thyroid gland.

  9. Benzene activates caspase-4 and -12 at the transcription level, without an association with apoptosis, in mouse bone marrow cells lacking the p53 gene

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    Yi, Jung-Yeon; Han, Jeong-Hee; Yoon, Byung-Il [Kangwon National University, School of Veterinary Medicine, Chuncheon, Gangwon (Korea); Hirabayashi, Yoko; Kodama, Yukio; Kanno, Jun [National Institute of Health Sciences, Division of Cellular and Molecular Toxicology, Center for Biological Safety and Research, Tokyo (Japan); Choi, Yang-Kyu [Konkuk University, College of Veterinary Medicine, Seoul (Korea); Inoue, Tohru [National Institute of Health Sciences, Biological Safety and Research Center, Tokyo (Japan)

    2009-08-15

    Benzene is a well-known environmental pollutant that can induce hematotoxicity, aplastic anemia, acute myelogenous leukemia, and lymphoma. However, although benzene metabolites are known to induce oxidative stress and disrupt the cell cycle, the mechanism underlying lympho/leukemogenicity is not fully understood. Caspase-4 (alias caspase-11) and -12 are inflammatory caspases implicated in inflammation and endoplasmic reticulum stress-induced apoptosis. The objectives of this study were to investigate the altered expression of caspase-4 and -12 in mouse bone marrow after benzene exposure and to determine whether their alterations are associated with benzene-induced bone marrow toxicity, especially cellular apoptosis. In addition, we evaluated whether the p53 gene is involved in regulating the mechanism, using both wild-type (WT) mice and mice lacking the p53 gene. For this study, 8-week-old C57BL/6 mice [WT and p53 knockout (KO)] were administered a benzene solution (150 mg/kg diluted in corn oil) via oral gavage once daily, 5 days/week, for 1 or 2 weeks. Blood and bone marrow cells were collected and cell counts were measured using a Coulter counter. Total mRNA and protein extracts were prepared from the harvested bone marrow cells. Then qRT-PCR and Western blotting were performed to detect changes in the caspases at the mRNA and protein level, respectively. A DNA fragmentation assay and Annexin-V staining were carried out on the bone marrow cells to detect apoptosis. Results indicated that when compared to the control, leukocyte number and bone marrow cellularity decreased significantly in WT mice. The expression of caspase-4 and -12 mRNA increased significantly after 12 days of benzene treatment in the bone marrow cells of benzene-exposed p53KO mice. However, apoptosis detection assays indicated no evidence of apoptosis in p53KO or WT mice. In addition, no changes of other apoptosis-related caspases, such as caspase-3 and -9, were found in WT or p53KO mice at the

  10. The Long-Term Conditions Questionnaire: conceptual framework and item development

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    Peters M

    2016-08-01

    Full Text Available Michele Peters,1 Caroline M Potter,1 Laura Kelly,1 Cheryl Hunter,1 Elizabeth Gibbons,1 Crispin Jenkinson,1 Angela Coulter,1 Julien Forder,2 Ann-Marie Towers,2 Christine A’Court,3,4 Ray Fitzpatrick1 1Health Services Research Unit, Nuffield Department of Population Health, University of Oxford, Oxford, 2Personal Social Services Research Unit, University of Kent, Canterbury, 3Nuffield Department of Primary Health Care Sciences, University of Oxford, Oxford, 4Broadshires Health Centre, Carterton, UK Purpose: To identify the main issues of importance when living with long-term conditions to refine a conceptual framework for informing the item development of a patient-reported outcome measure for long-term conditions.Materials and methods: Semi-structured qualitative interviews (n=48 were conducted with people living with at least one long-term condition. Participants were recruited through primary care. The interviews were transcribed verbatim and analyzed by thematic analysis. The analysis served to refine the conceptual framework, based on reviews of the literature and stakeholder consultations, for developing candidate items for a new measure for long-term conditions.Results: Three main organizing concepts were identified: impact of long-term conditions, experience of services and support, and self-care. The findings helped to refine a conceptual framework, leading to the development of 23 items that represent issues of importance in long-term conditions. The 23 candidate items formed the first draft of the measure, currently named the Long-Term Conditions Questionnaire.Conclusion: The aim of this study was to refine the conceptual framework and develop items for a patient-reported outcome measure for long-term conditions, including single and multiple morbidities and physical and mental health conditions. Qualitative interviews identified the key themes for assessing outcomes in long-term conditions, and these underpinned the development of the

  11. Role of Tamm-Horsfall protein and uromodulin in calcium oxalate crystallization

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    Carvalho M.

    2002-01-01

    Full Text Available One of the defenses against nephrolithiasis is provided by macromolecules that modulate the nucleation, growth, aggregation and retention of crystals in the kidneys. The aim of the present study was to determine the behavior of two of these proteins, Tamm-Horsfall and uromodulin, in calcium oxalate crystallization in vitro. We studied a group of 10 male stone formers who had formed at least one kidney stone composed of calcium oxalate. They were classified as having idiopathic nephrolithiasis and had no well-known metabolic risk factors involved in kidney stone pathogenesis. Ten normal men were used as controls, as was a group consisting of five normal women and another consisting of five pregnant women. Crystallization was induced by a fixed supersaturation of calcium oxalate and measured with a Coulter Counter. All findings were confirmed by light and scanning electron microscopy. The number of particulate material deposited from patients with Tamm-Horsfall protein was higher than that of the controls (P<0.001. However, Tamm-Horsfall protein decreased the particle diameter of the stone formers when analyzed by the mode of the volume distribution curve (P<0.002 (5.64 ± 0.55 µm compared to 11.41 ± 0.48 µm of uromodulin; 15.94 ± 3.93 µm and 12.45 ± 0.97 µm of normal men Tamm-Horsfall protein and uromodulin, respectively; 8.17 ± 1.57 µm and 9.82 ± 0.95 µm of normal women Tamm-Horsfall protein and uromodulin, respectively; 12.17 ± 1.41 µm and 12.99 ± 0.51 µm of pregnant Tamm-Horsfall protein and uromodulin, respectively. Uromodulin produced fewer particles than Tamm-Horsfall protein in all groups. Nonetheless, the total volume of the crystals produced by uromodulin was higher than that produced by Tamm-Horsfall protein. Our results indicate a different effect of Tamm-Horsfall protein and uromodulin. This dual behavior suggests different functions. Tamm-Horsfall protein may act on nucleation and inhibit crystal aggregation, while

  12. Interstitial diffuse radiance spectroscopy of gold nanocages and nanorods in bulk muscle tissues

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    Grabtchak S

    2015-02-01

    Full Text Available Serge Grabtchak,1,2 Logan G Montgomery,1 Bo Pang,3,4 Yi Wang,4,5 Chao Zhang,6,7 Zhiyuan Li,6,7 Younan Xia,4,8 William M Whelan1,91Department of Physics, University of Prince Edward Island, Charlottetown, PEI, Canada; 2Departments of Electrical and Computer Engineering, and Physics, Dalhousie University, Halifax, Canada; 3Department of Biomedical Engineering, Peking University, Beijing, People’s Republic of China; 4The Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA; 5Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing, People’s Republic of China; 6Laboratory of Optical Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, People’s Republic of China; 7College of Physics and Optoelectronics, South China University of Technology, Guangzhou, People’s Republic of China; 8School of Chemistry and Biochemistry, and School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, USA; 9Atlantic Veterinary College, Charlottetown, PEI, CanadaAbstract: Radiance spectroscopy was applied to the interstitial detection of localized inclusions containing Au nanocages or nanorods with various concentrations embedded in porcine muscle phantoms. The radiance was quantified using a perturbation approach, which enabled the separation of contributions from the porcine phantom and the localized inclusion, with the inclusion serving as a perturbation probe of photon distributions in the turbid medium. Positioning the inclusion at various places in the phantom allowed for tracking of photons that originated from a light source, passed through the inclusion’s location, and reached a detector. The inclusions with high extinction coefficients were able to absorb nearly all photons in the range of 650–900 nm, leading to a spectrally flat radiance signal. This signal could be

  13. Effectiveness of a physiotherapy-initiated telephone triage of orthopedic waitlist patients

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    Gilmore L

    2011-08-01

    Full Text Available Joanne Morris1, Karen Grimmer-Somers2, Saravana Kumar2, Karen Murphy3, Lisa Gilmore1, Bryan Ashman1, Chandima Perera1, Kathryn Vine1, Corinne Coulter11The Canberra Hospital, ACT Government Health Directorate, Canberra, ACT, Australia; 2International Centre for Allied Health Evidence, University of South Australia, Adelaide, SA, Australia; 3ACT Government Health Directorate, Canberra, ACT, AustraliaBackground: There is generally a lengthy wait on outpatient orthopedic waiting lists in Australian public hospitals to consult a specialist. Patients then wait again for surgery, if required. Patients with higher need are rarely prioritized, and there is the potential for increased morbidity for those who wait. There is generally no option of alternative care whilst waiting. This paper compares historical orthopedic outpatient clinic data with the outcomes of a physiotherapy-led initiative in one large Australian tertiary hospital.Methods: Two physiotherapists working within-scope conducted a telephone triage (October to December 2010 using a standard instrument for all new patients on the orthopedic waiting list. They were offered primary treatment options of retaining their appointment, being discharged, referral to a new model of assessment (multidisciplinary specialist clinic, or referral to physiotherapy. The outcomes were costs of the service, waiting time, and percentage of patients taking up management options. This was compared with a historical sample of new patients on the orthopedic waiting list (January to March 2009, whose treatment consumption was tracked longitudinally.Results: The telephone triage resulted in 16.4% patients being discharged directly (compared with 0.1% comparison sample. For approximately AU$17.00 per patient, the telephone triage process released 21 booked appointments on the outpatient clinic waiting list. Moreover, approximately 26% patients were referred directly to physiotherapy, which was not a primary management

  14. Mechanical damage of red blood cells by rotary blood pumps: selective destruction of aged red blood cells and subhemolytic trauma.

    Science.gov (United States)

    Sakota, Daisuke; Sakamoto, Ryuki; Sobajima, Hideo; Yokoyama, Naoyuki; Waguri, Satoshi; Ohuchi, Katsuhiro; Takatani, Setsuo

    2008-10-01

    In this study, mean cell volume (MCV), mean cell hemoglobin concentration (MCHC), and mean cell hemoglobin (MCH) were measured to quantify RBC damage by rotary blood pumps. Six-hour hemolysis tests were conducted with a Bio-pump BPX-80, a Sarns 15200 roller pump, and a prototype mag-lev centrifugal pump (MedTech Heart) using fresh porcine blood circulated at 5 L/min against a 100 mm Hg head pressure. The temperature of the test and noncirculated control blood was maintained at 37 degrees C. The normalized index of hemolysis (NIH) of each pump was determined by measuring the plasma-free hemoglobin level. The MCV was measured with a Coulter counter, and MCHC was derived from total hemoglobin and hematocrit. MCH was derived from MCV and MCHC. A multivariance statistical analysis (ANOVA) revealed statistically significant differences (n = 15, P < 0.05) in MCV, MCHC, and MCH between the blood sheared by the rotary blood pumps and the nonsheared control blood. Normalized to the control blood, the Bio-pump BPX-80 showed an MCV of 1.04 +/- 0.03, an MCHC of 0.95 +/- 0.04, and an MCH of 0.98 +/- 0.02; the mag-lev MedTech Heart had an MCV of 1.02 +/- 0.02, an MCHC of 0.97 +/- 0.02, and an MCH of 0.99 +/- 0.01; and the roller pump exhibited an MCV of 1.03 +/- 0.03, an MCHC of 0.96 +/- 0.03, and an MCH of 0.99 +/- 0.01. Per 0.01 increase in NIH, the BPX-80 showed a normalized MCV change of +10.1% and a normalized MCHC change of -14.0%; the MedTech Heart demonstrated a +6.9% MCV and -9.5% MCHC change; and the roller pump had a +0.5% MCV and -0.6% MCHC change. Due to shear in the pump circuits, the RBC increased while the MCHC decreased. The likely mechanism is that older RBCs with smaller size and higher hemoglobin concentration were destroyed fast by the shear, leaving younger RBCs with larger size and lower hemoglobin concentration. Subhemolytic trauma caused the intracellular hemoglobin to decrease due to gradual hemoglobin leakage through the micropores formed in the thinned

  15. Proliferative vitreo-retinal disorders: experimental models in vivo and in vitro.

    Science.gov (United States)

    Martini, B

    1992-01-01

    The aim of the present thesis was to develop, refine, and assess experimental models for the study of proliferative vitreo-retinal disorders. An intravitreal injection of a colloidal solution of microparticles was used in the primate eye to produce pathologic changes including intraocular cell invasion, cell proliferation, neovascularization, collagen synthesis, and tractional retinal detachment. In a separate primate model for laser-induced subretinal neovascularization, the origin and the occurrence of macrophages was evaluated. Examinations were performed using ophthalmoscopy, slit-lamp microscopy, light microscopy, and transmission electron microscopy. Cell cultures were employed to study the effects of vitreous humor and macrophages on the proliferation of cultured retinal pigment epithelial (RPE) cells and cultured fibroblasts using a Coulter counter. Morphologic changes were documented by phase micrography. A quantitative estimation of the extracellular matrix deposition of fibrous proteins by macrophage-modulated RPE cells as well as by vitreous-modulated RPE cells was done using enzymatic digestion and radioactive labeling techniques. A qualitative analysis of the types of collagen that was deposited in the extracellular matrices by vitreous modulated cultures was also made using indirect immunofluorescence. Using a newly developed RPE cell specific monoclonal antibody, the avidin-biotin-peroxidase labeling technique was finally employed to test the phenotypic epitope expression of macrophage-modulated and non-modulated RPE cells. A new experimental in vivo model for pathologic changes that characterize proliferative vitreo-retinal disorders was developed in the primate eye. In the model for laser-induced subretinal neovascularization, macrophages were shown to be principally recruited from the systemic circulation. Using cell cultures, it was found that both macrophage-conditioned medium and vitreous humor, separately or combined, exert mitogenic effects

  16. Growth inhibitory activity of extracts and compounds from Cimicifuga species on human breast cancer cells.

    Science.gov (United States)

    Einbond, Linda Saxe; Wen-Cai, Ye; He, Kan; Wu, Hsan-au; Cruz, Erica; Roller, Marc; Kronenberg, Fredi

    2008-06-01

    The purpose of this report is to explore the growth inhibitory effect of extracts and compounds from black cohosh and related Cimicifuga species on human breast cancer cells and to determine the nature of the active components. Black cohosh fractions enriched for triterpene glycosides and purified components from black cohosh and related Asian species were tested for growth inhibition of the ER(-) Her2 overexpressing human breast cancer cell line MDA-MB-453. Growth inhibitory activity was assayed using the Coulter Counter, MTT and colony formation assays. Results suggested that the growth inhibitory activity of black cohosh extracts appears to be related to their triterpene glycoside composition. The most potent Cimicifuga component tested was 25-acetyl-7,8-didehydrocimigenol 3-O-beta-d-xylopyranoside, which has an acetyl group at position C-25. It had an IC(50) of 3.2microg/ml (5microM) compared to 7.2microg/ml (12.1microM) for the parent compound 7,8-didehydrocimigenol 3-O-beta-d-xylopyranoside. Thus, the acetyl group at position C-25 enhances growth inhibitory activity. The purified triterpene glycoside actein (beta-d-xylopyranoside), with an IC(50) equal to 5.7microg/ml (8.4microM), exhibited activity comparable to cimigenol 3-O-beta-d-xyloside. MCF7 (ER(+)Her2 low) cells transfected for Her2 are more sensitive than the parental MCF7 cells to the growth inhibitory effects of actein from black cohosh, indicating that Her2 plays a role in the action of actein. The effect of actein on Her2 overexpressing MDA-MB-453 and MCF7 (ER(+)Her2 low) human breast cancer cells was examined by fluorescent microscopy. Treatment with actein altered the distribution of actin filaments and induced apoptosis in these cells. These findings, coupled with our previous evidence that treatment with the triterpene glycoside actein induced a stress response and apoptosis in human breast cancer cells, suggest that compounds from Cimicifuga species may be useful in the prevention and

  17. Effect of chronic alcohol consumption on the red blood cell count and RBC indices in the HIV infected patients on d4T/3TC/NVP drug regimen in Uganda

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    Godfrey S. Bbosa

    2013-10-01

    Full Text Available Alcohol consumption is common problem in Uganda. Among the types of alcohols consumed include beers, spirits, liqueurs, wines and traditional brew. These alcohols are easily accessible and consumed by many people including the HIV infected patients who are on the d4T/3TC/NVP regimen. The aim of this study was to determine the effect of chronic alcohol intake on the red blood cell count (RBC and the RBC indices in the HIV-infected patients on d4T/3TC/NVP regimen. It was a case control study that used a repeated measures design model where serial measurements of the red blood cell count (RBC and RBC indices were determined at 3 month interval for 9 months. A total of 41 HIV infected patients were recruited and grouped into two arms; the control group had 21 patients and the chronic alcohol group had 20 patients. The RBC and RBC indices of the whole blood were determined using automated hematological Coulter CBC-5 Hematology Analyzer system using standard procedures. The data was sorted into alcohol-use self reporting by WHO AUDIT tool and alcohol-use biomarkers groups. It was analysed using the SAS 2003 version 9.1 statistical package with the repeated measures fixed model. The means were compared using the student t-test. The mean MCV and MCH values in the chronic alcohol use group were higher than in the control group and there was a significant difference between the 2 groups (p<0.05 for both the WHO AUDIT tool group and chronic alcohol use biomarkers group. The mean RBC count, Hct, HGB and MCHC values in both the control and chronic alcohol use groups were within the normal reference ranges for both groups though the trend was lower in alcohol group. Chronic alcohol use affects the RBC and RBC indices in the HIV infected patients on d4T/3TC/NVP treatment regimen. [Int J Basic Clin Pharmacol 2013; 2(5.000: 528-536

  18. Polymerization time for a microwave-cured acrylic resin with multiple flasks Tempo de polimerização de resina acrílica em microondas, utilizando múltiplas muflas

    Directory of Open Access Journals (Sweden)

    Daniela Maffei Botega

    2004-03-01

    Full Text Available This study aimed at establishing the polymerization time of a microwave-cured acrylic resin (AcronTM MC, simultaneously processing 2, 4, and 6 flasks. Required time was measured according to the parameters: monomer release in water, Knoop hardness, and porosity. Samples were made with AcronTM MC in different shapes: rectangular (32 x 10 x 2.5 mm for monomer release and porosity; and half-disc (30 mm in diameter x 4 mm in height for Knoop hardness. There were four experimental groups (n = 24 per group: G1 one flask (control; G2 two flasks; G3 four flasks, and G4 six flasks. At first, polymerization protocol was similar for all groups (3 min/450 W. Time was then adjusted for G2, G3, and G4, based on monomer release evaluation in the control group, obtained by spectrophotometer Beckman DU-70, with emitting wave of 206 nm. Knoop hardness test was performed using a Shimadzu HMV 2000 hardness tester, and 10 indentations were performed on each specimen's surface. Porosity was assessed after specimens were immersed in black ink and the pores counted in a microscope. Results showed that the complete polymerization of the resin occurred in 4.5 min for two flasks (G2; 8.5 min for four flasks (G3; and 13 min for six flasks (G4, all with 450 W. Statistical analysis revealed that the number of flasks does not interfere with polymerization, Knoop hardness, and porosity of the resin. Results showed that polymerization of microwave-curing resin with more than one flask is a viable procedure, as long as polymerization time is adjusted.O objetivo deste estudo foi o de determinar os tempos necessários para a polimerização padrão de uma resina acrílica em microondas, utilizando várias muflas simultaneamente. Os tempos necessários foram aferidos por parâmetros como monômeros liberados em água, dureza Knoop e porosidade. As amostras, confeccionadas em resina AcronTM MC, apresentavam as seguintes dimensões: para os parâmetros monômero residual e porosidade

  19. Atividade elétrica cerebral do rato com lesões da formação reticular mesencefálica Electrocorticographic study of the rats's bram after lesioning of the midbrain reticular formation

    Directory of Open Access Journals (Sweden)

    Walter C. Pereira

    1970-09-01

    Full Text Available No presente estudo foram utilizados 73 ratos em preparações agudas e crônicas, nas quais lesamos a formação reticular mesencefálica com corrente contínua (3,5 a 4,0 mA durante 5 a 10 segundos. O eletródio ativo era implantado estereotàxicamente segundo as coordenadas de König e Klippel. As lesões eram feitas parcial ou totalmente, uni ou bilateralmente, e em todos os animais procedeu-se ao controle histológico das áreas lesadas, usando-se o método de Weil. O registro da atividade elétrica cortical foi feito com polígrafo Beckman de 4 canais, utilizando-se derivações bipolares curtas (1mm com eletródios esféricos de platina. As experiências permitiram as seguintes conclusões: 1 — As características eletrofisiológicas dos fusos que ocorrem após lesões da formação reticular mesencefálica são muito semelhantes às dos fusos espontâneos e barbitúricos, inclusive quanto à projeção cortical. Quanto à duração dos potenciais que os constituem, contudo, notamos que a faixa de variação era mais centuada (20 a 80 ms, o que pode ser atribuído à maior complexidade dos potenciais do cérebro isolado, possivelmente pela falta de ação cronadora da formação reticular sobre o sistema sincronizador talâmico. 2 — Os mecanismos envolvidos na gênese dos fusos do sono barbitúrico ou espontâneo e os do cérebro isolado são, pelo menos em parte, dependentes do bloqueio da formação reticular mesencefálica. 3 — A formação reticular mesencefálica ativa preferencialmente o hemisfério cerebral homolateral; o contingente cruzado talvez seja mobilizado somente quando estímulos alertantes intensos atingem o tegmento mesencefálico. 4 — Além da formação reticular mesencefálica deve haver outros mecanismos ativadores corticais, visto que, em preparações agudas de cérebro isolado, observamos: a surtos de curta duração de atividade dessincronizada; b oscilações freqüentes do ECoG durante o registro

  20. The comparison of reticulocyte parameters on the XN-1000 and LH780 hematology analyzer%LH780与XN-1000血液分析仪的网织红细胞参数结果比较

    Institute of Scientific and Technical Information of China (English)

    费选文; 张苏伟; 陈书裕

    2015-01-01

    Objective To analyze the comparability and correlation of reticulocyte parameters on the Beckman‐Coulter LH780 (LH780)and Sysmex XN‐1000(XN‐1000)hematology analyzers .Methods 80 blood samples were measured by the two instru‐ments ,the RBC count ,percentage of Ret(Ret% ) ,Ret value(Ret#)and immature reticulocyte fraction(IRF) were analyzed ,and the correlation of Ret% ,Ret# and IRF between two instruments were also analysed .The correlation between percentage of high laser reticulocyte(HLR% ) of LH780 and percentage of middle fluorescent reticulogyte(MFR% )+percentage of high fluorescent reticu‐locyte(HFR% ) of XN‐1000 were compared using two calculation methods of each instrument ,and the application value of HLR%were analysed .Results No significant differences were founded in RBC count ,Ret% ,and Ret# between the two instruments(P>0 .05) ,while there was statistical difference in IRF between the the two instruments (P<0 .05) .The relative deviation coincidence rate of RBC count was 97 .5% ,the correlation coefficent (r) of Ret% ,Ret# and IRF were 0 .912 ,0 .895 and 0 .666 respectively . There were statistical differences and correlations between HLR% and MFR% + HFR% when using calculation method of XN‐1000 and LH780 respectively(r were 0 .666 and 0 .767 respectively ,P<0 .05) .Conclusion The RBC count could meet the matc‐hing requirement ,and the Ret% and Ret # may be highly correlated on the two instruments .While the reference range of IRF should be established in each instrument .%目的:分析Beckman‐Coulter LH780与Sysmex XN‐1000两台血液分析仪的网织红细胞(Ret)等参数的可比性和相关性。方法用上述两台仪器分别检测80份血常规标本,比较红细胞(RBC )计数、Ret百分率(Ret%)、Ret绝对值(Ret#)和未成熟网织红细胞指数(IRF),并对Ret%、Ret#和IRF进行相关性分析。将LH780的高散射光网织红细胞百分比(HLR%)和XN‐1000

  1. 4LT-A型错行作业挖掘甜菜联合收获机研制与试验%Development and experiment of 4LT-A staggered-dig sugar beer combine

    Institute of Scientific and Technical Information of China (English)

    顾峰玮; 胡志超; 吴惠昌; 彭宝良; 高学梅; 王申莹

    2014-01-01

    Sugar beet was the most important source of sugar in the sugar industry, with the sugar amounting to about 17% of China's total sugar production. As a seasonal operation, sugar beet harvesting in China was still mainly manual work, having the problems of high labor intensity, low efficiency, great harvest losses and huge occupation of farming and so on, which constrained the further development of the sugar beet industry. Mechanized harvesting was the inevitable development trend of sugar beet harvest. Beet harvest mechanization development had lagged in our country for a long time. And we were lack of a thorough system research about sugar beet harvest mechanization technology, especially the advanced combine technology. The current domestic sugar beet harvesters were basically simple sectional operating machinery. Sugar beet combine harvesters were mainly imported from abroad, having high automation and intelligence degree and advanced performance. But imported machines had the problems of poor adaptability and high prices. The domestic beet combines were still in the prototype development stage, having a great gap with the advanced foreign technology. In order to improve the mechanization level and reduce the costs of sugar beet harvest machine, a staggered-dig sugar beet combine was developed, fully digesting and absorbing advanced technology, combined with the sugar beet grain planting scale, economic conditions, and the present situation of domestic machinery manufacturing level. The overall structure includes traction frame, row detection mechanism, round coulter, copying cut top institution, digging and conveying institution, side of threshing mechanism, walking system, cage type rotary lifting device, conveying equipment, main frame, stock bin, top conveyer and so on. The sugar beet combine adopted the key technologies such as top contour cutting, auto-follow row system, hydraulic technology, sensor signal acquisition technology and microprocessor control

  2. Métodos de detección de la mastitis bovina (Methods of detection of the bovine mastitis

    Directory of Open Access Journals (Sweden)

    Bedolla, CC

    2007-09-01

    Full Text Available ResumenEste artículo es una revisión bibliográfica sobre los métodos de detección de la mastitis bovina que se utilizan más comúnmente en el mundo. La mastitis bovina es la inflamación de la glándula mamaria causada por una infección por patógenos. Es una de las enfermedades más frecuentes de la producción que afecta a la industria lechera en todo el mundo. Puede presentarse de manera clínica y subclínica. La mastitis subclínica es de larga duración y es mucho más frecuente que la mastitis clínica. Dentro de los métodos que se usan con mayor frecuencia a nivel de campo para diagnosticar mastitis clínicas, se encuentran el método de observación y palpación de la ubre y las pruebas físicas, como la prueba de escudilla de ordeño, prueba del paño negro y taza probadora. Las pruebas químicas, como la prueba de conductividad eléctrica de la leche, papel indicador de mastitis y prueba de whiteside que sirven también para diagnosticar mastitis clínicas y subclínicas. Las pruebas biológicas, como son la prueba de California para mastitis, la prueba de Wisconsin, el diagnóstico bacteriológico por los métodos de aislamiento, cultivo, tinción, pruebas bioquímicas e identificación y el conteo de células somáticas por microscopia directa y el somaticell. Otros métodos utilizados actualmente por su rapidez y efectividad son los electrónicos como el fossomatic y el counter coulter, los cuales tienen una aplicación universal sobre todo en laboratorios de control lechero o dedicados al diagnóstico e investigación de la mastitis y el DeLaval cell counter. Los métodos de detección de mastitis son una herramienta que permite identificar el tipo de infección clínica o subclínica que puede presentarse dentro de un hato lechero, por lo que el método que se elija para determinar las pruebas será esencial para tener un diagnóstico más preciso.AbstractThis article is a bibliographical revision on the methods of

  3. Atributos químicos de um latossolo bruno sob sistema plantio direto em função da estratégia de adubação e do método de amostragem de solo Chemical properties of a haplohumox under no-tillage as related to fertilization strategies and soil sampling method

    Directory of Open Access Journals (Sweden)

    Volnei Pauletti

    2009-06-01

    greater in the no-tillage system because there is no homogenization of the soil surface layer. This study evaluated the effect of fertilization strategies on soil chemical properties at distinct soil depths after three and six years from the beginning of the experiment in a Haplohumox under long-term no-tillage. Besides, the variation of these properties considering two methods of soil sampling, with and without inclusion of the seeding row, was evaluated. Ten treatments were applied, nine considering P sources (rock phosphate and triple superphosphate, row preparation equipment (double disc opener and coulter opener, fertilizer placement (in-row and broadcast, time of application (summer and winter and one control treatment, without fertilizer. The measured soil chemical properties were higher in the surface layers and depth distribution, except for P, was not influenced by the fertilization strategies. The soil P values were the lowest in the 0-5 cm layer where the coulter opener equipment had been continuously used for more than three years and no fertilizer was applied. With the inclusion of the sowing row in the soil sampling, higher K, Ca and CEC values were observed. The methodologies of soil sampling, with and without the inclusion of the sowing row and the 0-10 and 0-20 cm sampling layers resulted in similar fertilizer and lime recommendations for areas under long-term no-tillage.

  4. 131I对Graves病外周血粒细胞集落刺激因子和白细胞的影响%Effects of 131I treatment on the circulating granulocyte colony-stimulating factor and leucocyte levels in patients with Graves' disease

    Institute of Scientific and Technical Information of China (English)

    蔡强军; 潘芙蓉; 张琦; 李焕斌; 夏玉祥; 谢克俭

    2011-01-01

    Objective To observe the effects of 131I treatment on circulating granulocyte colonystimulating factor (G-CSF) and leucocyte levels of patients with Graves' disease (GD).Methods Enzyme-linked immunosorbent assay (ELISA),coulter three assortments,and radioimmunoassay were used to test the levels of circulating G-CSF,leucocytes and thyroid hormones of 65 incipient and untreated GD patients,all females,aged 21 -50,43 with normal leucocyte level and 22 with leucopenia before and after 131I treatment.Thirty age-matched healthy female subjects were used as controls.Results Before 131I treatment,the serous G-CSF level of the GD patients with normal leucocyte level was (28.4 ± 11.7)μg/L,significantly higher than that of the control [ ( 18.3 ± 6.98) μg/L,t =2.376,P < 0.05 ].The serous G-CSF level of the GD patients with leucopenia was (40.1 ± 13.8 ) μg/L,significantly higher than that of the patients with normal leucocyte level ( t =2.788,P < 0.01 ) and that of the control ( t =3.672,P<0.01 ).180 d after the initiation of 131 I treatment,the G-CSF level of the patients with normal leucocyte level was (18.9 ± 8.32) μg/L,not significantly different from that of the normal controls,however,the G-CSF level of the GD patients with leucopenia was (25.7 ± 11.5) μg/L,still significantly higher than that of the normal control (t =2.103,P < 0.05).The serous G-CSF level was negatively correlated with the titer of leucocyte ( r =- 0.38,P < 0.05 ),however,not significantly correlated with such clinical parameters,as free triiodothyronine (FT3),free thyroxine (FT4) and thyrotropin-stimulating hormone (TSH).Conclusions Abnormal increment of G-CSF is observed in the GD patients,which may be related to the decrease of leucocyte.Effectively suppressing the auto-immune status in the GD patients,131I treatment is a safe and reliable therapy for GD patients with leucopenia and should be used as early as possible.%目的 探讨131I治疗Graves病(GD)对患者外周血粒细

  5. 烟碱上调大鼠脑纹状体多巴胺D1受体mRNA表达诱导其行为改变%Chronic nicotine induces the changes of locomotor activities in rats by increasing the mRNA levels of dopamine D1 receptor in striatum

    Institute of Scientific and Technical Information of China (English)

    陈涛; 郭纪锋; 唐北沙; 廖小平; 文国强; 严新翔; 江泓; 张玉虎; 龙志刚; 欧阳锋

    2006-01-01

    背景:研究提示,烟碱对帕金森病小鼠具有神经保护效应,临床试验也观察到,帕金森病患者在吸烟过程中其震颤、僵直、运动减少等症状减轻,但其作用机制目前尚不明了.目的:观察烟碱对大鼠脑纹状体多巴胺D1,D2受体mRNA表达的影响,分析烟碱对帕金森病神经保护效应的作用机制.设计:完全随机分组设计,对照试验.单位:中南大学湘雅医院神经病学研究所.材料:选用健康雄性清洁级SD大鼠24只,10周龄,体质量180~200 g.主要试剂及仪器:烟碱(美国Sigma公司)、反转录试剂盒(美国MBI公司)、聚合酶链反应热循环仪(美国Beckman)、全自动紫外分光光度计(美国Beckman Du-70)、图像分析处理系统(美国Stratagene Eagle Eye Ⅱ型).方法:实验于2001-07/2002-07在中南大学湘雅医院神经病学研究所实验室完成.①按随机抽签法将大鼠随机分为2组:对照组、烟碱组,每组12只.分别皮下注射生理盐水、烟碱溶液4 mg/(kg·d),2次/d,共14 d.注射药物后0.5 h观察大鼠行为活动,包括定型活动、走动活动、攀爬行为、旋转行为、理毛行为、张口行为、呕吐行为等,观察时间为0.5 h.②于末次注射药物后0.5 h处死动物,快速分离纹状体,提取总RNA,采用美国MBI反转录试剂盒反转录cDNA,在特定条件下进行聚合酶链反应扩增,将电泳凝胶在Eagle EyeⅡ图像处理系统上分析计算出多巴胺D1,D2受体及内对照β-actin吸光度(A值),检测大鼠脑纹状体多巴胺Di,D2受体mRNA的表达.③两样本均数比较采用t检验.主要观察指标:①大鼠行为学改变.②大鼠脑纹状体多巴胺Di,D2受体mRNA的表达.结果:大鼠24只均进入结果分析.①烟碱组大鼠于用药第3天,出现走动增多,易激惹,定型活动明显,并于第7~14天达到高峰;对照组则无明显变化.②大鼠脑纹状体所有标本总RNA A260/280均>1.8,经12g/L琼脂糖浆电泳显示无明显降解;通

  6. To discuss significance of homocysteine and beta2-microglobulin detection in the early diagnosis of diabetic nephropathy%探讨同型半胱氨酸和β2-微球蛋白检测在糖尿病肾病早期诊断的意义

    Institute of Scientific and Technical Information of China (English)

    龙训琴

    2013-01-01

    目的:通过对糖尿病肾病(DN)不同病期患者的血清同型半胱氨酸(Hcy)和β2-微球蛋白(β2-MG)的检测,探讨血清Hcy和β2-MG水平改变在DN诊断中的临床价值。方法:在美国贝克曼AU2700全自动生化分析仪上采用循环酶法和胶乳增强免疫比浊法检测实验组和对照组血液样本中Hcy和β2-MG的水平,同时检测与肾功能密切相关的血清学指标尿素(UREA)和肌酐(CREA)的含量。结果:实验组血清Hcy、β2-MG、UREA和CREA含量明显高于对照组,两组比较差异有统计学意义或高度统计学意义(p<0.05或p<0.01);Ⅰ期、Ⅱ期、Ⅲ期、Ⅳ期检测Hcy和β2-MG浓度水平呈递增性升高,Ⅱ期、Ⅲ期和Ⅳ期患者血清中Hcy和β2-MG的含量明显高于Ⅰ期患者,与Ⅰ期患者检测结果比较,差异有统计学意义(P<0.05)。结论:联合检测血清Hcy和β2-MG水平有助于DN的早期诊断,检测结果能够及早为临床治疗以及临床采取正确的治疗方案提供具有参考价值的诊断依据。%Objective:Based on diabetic nephropathy (DN) of different stage disease patients serum homocysteine (Hcy) and beta 2-microglobulin (beta 2-MG) test, to observe the change of serum Hcy and beta 2-MG level clinical value in the diagnosis of DN. Methods:Beckman AU2700 automatic biochemical analyzer in the United States on circulating enzymatic and latex enhanced immune turbidimetric method is used to test the experimental group and control group in blood samples of Hcy and beta 2-MG level, at the same time test and serological indexes of renal function is closely related to the UREA (UREA) and creatinine (CREA) content.Results:Experimental serum Hcy, beta 2, MG, UREA and CREA content is significantly higher than the control group, two groups of comparisons difference was statistically significant or highly significant (p < 0.05 or p < 0.01); Ⅰ stage, stageⅡ,Ⅲperiod,Ⅳdetection of Hcy and beta 2-increasing MG

  7. Enhanced UV Absorption in Carbonaceous Aerosols during MILAGRO and Identification of Potential Organic Contributors.

    Science.gov (United States)

    Mangu, A.; Kelley, K. L.; Marchany-Rivera, A.; Kilaparty, S.; Gunawan, G.; Gaffney, J. S.; Marley, N. A.

    2007-12-01

    Measurements of aerosol absorption were obtained as part of the MAX-Mex component of the MILAGRO field campaign at site T0 (Instituto Mexicano de Petroleo in Mexico City) during the month of March, 2006 by using a 7- channel aethalometer (Thermo-Anderson). These measurements, obtained at 370, 470, 520, 590, 660, 880, and 950 nm at a 5 minute time resolution, showed an enhanced absorption in the UV over that expected from carbon soot alone. Samples of fine atmospheric aerosols (less than 0.1micron) were also collected at site T0 and T1 (Universidad Technologica de Tecamac, State of Mexico) from 5 am to 5 pm (day) and from 5 pm to 5 am (night) during the month of March 2006. The samples were collected on quartz fiber filters with high volume impactor samplers. The samples have been characterized for total carbon content (stable isotope ratio mass spectroscopy) and natural radionuclide tracers (210Pb, 210Po, 210Bi, 7Be, 13C, 14C, 40K, 15N). Continuous absorption spectra of these aerosol samples have been obtained in the laboratory from 280 to 900nm with the use of an integrating sphere coupled to a UV-visible spectrometer (Beckman DU with a Labsphere accessory). The integrating sphere allows the detector to collect and spatially integrate the total radiant flux reflected from the sample and therefore allows for the measurement of absorption on highly reflective or diffusely scattering samples (1). The continuous spectra also show an enhanced UV absorption over that expected from carbon soot and the general profiles are quite similar to those observed for humic and fulvic acids found as colloidal materials in surface and groundwaters (2), indicating the presence of humic-like substances (HULIS) in the fine aerosols. The spectra also show evidence of narrow band absorbers below 400 nm typical of polycyclic aromatics (PAH) and nitrated aromatic compounds. Spectra were also obtained on NIST standard diesel soot (SRM 2975), NIST standard air particulate matter (SRM 8785

  8. Infrared Absorption by Atmospheric Aerosols in Mexico City during MILAGRO.

    Science.gov (United States)

    Kelley, K. L.; Mangu, A.; Gaffney, J. S.; Marley, N. A.

    2007-12-01

    Past research in our group using cylindrical internal reflectance spectroscopy has indicated that aqueous aerosols could contribute to the radiative warming as greenhouse species (1,2). Although aerosol radiative effects have been known for sometime and are considered one of the major uncertainties in climate change modeling, most of the studies have focused on the forcing due to scattering and absorption of radiation in the uv- visible region (3). Infrared spectral information also allows the confirmation of key functional groups that are responsible for enhanced absorption observations from secondary organics in the uv-visible region. This work extends our efforts to evaluate the infrared absorption by aerosols, particularly organics, that are now found to be a major fraction of urban and regional aerosols in the 0.1 to 1.0 micron size range and to help identify key types of organics that can contribute to aerosol absorption. During the MILAGRO campaign, quartz filter samples were taken at 12-hour intervals from 5 am to 5 pm (day) and from 5 pm to 5 am (night) during the month of March 2006. These samples were taken at the two super-sites, T-0 (Instituto Mexicano de Petroleo in Mexico City) and T-1 (Universidad Technologica de Tecamac, State of Mexico). The samples have been characterized for total carbon content (stable isotope mass spectroscopy) and natural radionuclide tracers, as well as for their UV-visible spectroscopic properties by using integrating sphere diffuse reflectance spectroscopy (Beckman DU with a Labsphere accessory). These same samples have been characterized in the mid and near infrared spectral ranges using diffuse reflection spectroscopy (Nicolet 6700 FTIR with a Smart Collector accessory). Aerosol samples were removed from the surfaces of the aerosol filters by using Si-Carb sampler. The samples clearly indicate the presence of carbonyl organic constituents and the spectra are quite similar to those observed for humic and fulvic acids

  9. H-ZSM-5催化环己酮肟制ε-己内酰胺反应机理的O NIO M研究%Reaction Mechanism of Cyclohexanone Oxime to ε-Caprolactam Catalyzed over H-ZSM-5:An ONIOM Study

    Institute of Scientific and Technical Information of China (English)

    王寒露; 周建敏; 周如金

    2014-01-01

    The Backman rearrangement reaction mechanism of cyclohexanone oxime to ε-caprolactam over the H-ZSM-5 zeolite was investigated by the ONIOM and DFT methods.All geometry structures optimization were em-ployed ONIOM (B3LYP/6-31+G(d):PM3)method.Based on the optimal configuration,a high single point cal-culation of complete 46T cluster model were carried out by a variety of density functionals,such as B3LYP/6-31+G(d),PBE/6-31+G(d),M062X/6-31+G(d)andωB97XD/6-31+G(d).Additional dispersion corrections were calculated by dftd3 program to describe the weak interaction in zeolites for B3LYP,PBE and M062X functionals. The calculation results showed that the precision of the DFT functionals for dispersion correction was greatly im-proved,which were comparable with the MP2 method.The rate determining step was the first step 1 ,2-H shift and rearrangement step,and internal reaction energy barrier was 44.5 kcal/mol.The reaction rate constants indicated that the Beckman rearrangement reaction was very slowly on the H-ZSM-5 at 293-393 K.When the temperature reached 423 K,the forward and reverse reaction rate were about the same,and the reaction occurred.When reached 623 K,the reaction rate constant was 130 1/s.%采用ONIOM和DFT方法研究了H-ZSM-5分子筛催化环己酮肟制ε-己内酰胺的贝克曼重排反应机理.所有的构型优化使用ONIOM(B3LYP/6-31+G(d):PM3)方法进行,并在此基础上对得到的最优构型应用多种密度泛度方法,如B3LYP/6-31+G(d),PBE/6-31+G(d),M062X/6-31+G(d)和ωB97XD/6-31+G(d)进行完整46T簇模型的单点能计算.B3LYP,PBE和M062X泛函使用dftd3程序计算了额外色散校正,以考虑分子筛内部的弱相互作用.计算结果显示,经色散校正的DFT方法的计算精度被大大提高,达到一个与MP2方法相媲美的精度.反应的决速步是由第一步1,2-H转移和重排步共同决定,内部反应能垒为44.5 kcal/mol.

  10. Atividade elétrica cerebral do rato com lesões da formação reticular mesencefálica

    Directory of Open Access Journals (Sweden)

    Walter C. Pereira

    1970-09-01

    Full Text Available No presente estudo foram utilizados 73 ratos em preparações agudas e crônicas, nas quais lesamos a formação reticular mesencefálica com corrente contínua (3,5 a 4,0 mA durante 5 a 10 segundos. O eletródio ativo era implantado estereotàxicamente segundo as coordenadas de König e Klippel. As lesões eram feitas parcial ou totalmente, uni ou bilateralmente, e em todos os animais procedeu-se ao controle histológico das áreas lesadas, usando-se o método de Weil. O registro da atividade elétrica cortical foi feito com polígrafo Beckman de 4 canais, utilizando-se derivações bipolares curtas (1mm com eletródios esféricos de platina. As experiências permitiram as seguintes conclusões: 1 — As características eletrofisiológicas dos fusos que ocorrem após lesões da formação reticular mesencefálica são muito semelhantes às dos fusos espontâneos e barbitúricos, inclusive quanto à projeção cortical. Quanto à duração dos potenciais que os constituem, contudo, notamos que a faixa de variação era mais centuada (20 a 80 ms, o que pode ser atribuído à maior complexidade dos potenciais do cérebro isolado, possivelmente pela falta de ação cronadora da formação reticular sobre o sistema sincronizador talâmico. 2 — Os mecanismos envolvidos na gênese dos fusos do sono barbitúrico ou espontâneo e os do cérebro isolado são, pelo menos em parte, dependentes do bloqueio da formação reticular mesencefálica. 3 — A formação reticular mesencefálica ativa preferencialmente o hemisfério cerebral homolateral; o contingente cruzado talvez seja mobilizado somente quando estímulos alertantes intensos atingem o tegmento mesencefálico. 4 — Além da formação reticular mesencefálica deve haver outros mecanismos ativadores corticais, visto que, em preparações agudas de cérebro isolado, observamos: a surtos de curta duração de atividade dessincronizada; b oscilações freqüentes do ECoG durante o registro

  11. Expression of nerve growth factor mRNA in splenic lymphocytes of bronchial asthma rats and its influencing actors

    Institute of Scientific and Technical Information of China (English)

    Jihong Dai; Yonghong Wang; Haixia He

    2008-01-01

    BACKGROUND: Previous research has proved that nerve growth factor (NGF) participates in the onset of asthma by the induction of neurogenic inflammation.OBJECTIVE: To investigate the effect of interleukin-13 (IL-13) and interferon-γ(IFN-γ) on the expression of NGF mRNA in the splenic lymphocytes of bronchial asthma rats.DESIGN, TIME AND SETTING: The experiment, a completely randomized study based on cellular immunology, was performed in the Laboratory of Neurology in Chongqing Medical University and the Department of Clinical Pharmacy in College of Clinical Medicine, Chongqing Medical University (Chongqing, China) from January 2006 to April 2007.MATERIALS: Four adult male Wistar rats were used in this study. Rat IL-13, IFN-γprobe and the total RNA extraction kit were produced by Shanghai Sangon Biological Technology & Services Co., Ltd (China). The NGF ELISA kit was a product of Wuhan Boster Bioengineering Co., Ltd (China). A Du-70 automatic UV spectrophotometer was produced by Beckman Company (USA).METHODS: Rats were subjected to 1-mL intraperitoneal injections each containing 100 mg of ovalbumin, and were sensitized by using antigen solution, which was sensitized with 5×109 Bacillus pertussis and 100 mg aluminum hydroxide powder. Four rats were challenged with 1% ovalbumin using an ultrasonic nebulizer for 60 minutes to establish an asthmatic model. After rats were anesthetized, splenic lymphocytes were isolated and cultured in medium, which was supplemented with IL-13 or IFN-γ, for 0, 12, 24 or 48 hours. A parallel study was conducted with cultured splenic lymphocytes, which were divided into a control group, an IL-13 group and an IFN-γ group. Culture medium was added with different concentrations of IL-13 (10, 50, 100 μg/L) and IFN-γ (1, 10, 50 μg/L); 24 hours later, all samples were harvested.MAIN OUTCOME MEASURES: The expression levels of NGF mRNA were detected by reverse transcription-polymerase chain reaction.RESULTS: In the control group, the

  12. 2702例孕妇中期胎儿唐氏征筛查结果的分析%Screening Analysis of Down's Syndromes in 2702 cases on Middle Period Pregnancy

    Institute of Scientific and Technical Information of China (English)

    江先雨; 刘志伟; 叶飞娣

    2012-01-01

      目的分析2702例孕妇中期胎儿唐氏征筛查结果,探讨孕妇中期胎儿唐氏征产前筛查的临床意义.方法采用贝克曼库尔特全自动化学发光酶免分析仪及原装试剂,对孕14~20+6周的孕妇血清进行甲胎蛋白(AFP)、人绒毛膜促性激素β亚基(β-HCG)及游离雌三醇(uE3)检测,并将孕妇的相关信息和检测的 AFP、β-HCG、uE3结果输入“腾程筛查软件”中,进行三联检测的结果运算,得出一个概率指标.结果唐氏综合征(DS)筛查结果高危例数有65例,总阳性率为2.41%;其中:20~24岁年龄组的孕妇唐氏综合征筛查结果高危例数有11例(1.76%)25-29岁年龄组的孕妇唐氏综合征筛查结果高危例数有26例(1.93%);30-35岁年龄组的孕妇唐氏综合征筛查结果高危例数有9例(2.77%);>35岁年龄组的孕妇唐氏综合征筛查结果高危例数有19例(4.73%).结论在中期孕妇进行唐氏综合征三联筛查是必要的,能够提高唐氏患儿的早期发现和诊断,是降低唐氏患儿出生率的有效方法.%  Objective To discussed the middle for pregnant women fetal down the clinical significance of prenatal screening,2702 cases were analyzed for pregnant women fetus down the middle screening results. Methods Used the Beckman Kurt automatic chemiluminescence enzyme from analyzer and original reagent to detection of AFP, β-HCG and uE3 content in pregnant women of 14~20+6 weeks. Pregnant women's information and AFP, β-HCG and uE3 assessment input "Tengcheng software" for joint screening, get a probability index. Results Down syndrome (DS) screening high-risk number results 65 cases, the total prevalence rate was 2.41%; Among them: 20~24 years old age group of pregnant women down's syndrome screening results have high-risk number 11 cases(1.76%); 25~29 age group of pregnant women down's syndrome screening results high-risk number 26 cases (1.93%); 30~35 years old age group of pregnant women down

  13. 咳嗽变异性哮喘儿童检测血清总IgE含量及过敏原特异性IgE的临床意义%THE CLINICAL SIGNIFICANCE OF DETECTION ON SERUM TOTAL IGE AND ALLERGEN SPECIFIC IGE IN COUGH VARIANT ASTHMA CHILDREN

    Institute of Scientific and Technical Information of China (English)

    林芳辉; 梁锋; 韩景辉; 符生苗

    2012-01-01

    [Objective] Through the detection on serum total IgE content and allergens specificity IgE 132 of 132 cases of cough variant asthma and other 56 cases of chronic cough {control group) , to investigate the application value of serum total IgE content ami specific allergen in clinical diagnosis, treatment and prevention of cough variant asthma. [Methods] Used I-niled Slates DX1800 beckman chemiluminescence to detect total IgE content, used Gentian ormond imprinting method to detect 20 kinds of allergens specificity IgE. [Resiltts] The positive rale of CVA total IgE was 87.1%. Specific IgE of 78.8%. The total IgE in the control group was 8.9% , positive rate of specificity IgE of 7.1%. CVA inhaled allergens were highest, der-malophagoides pUfronyssinus/dermalopbagoides farinue, mould combination, house dust were 55.3%, 43.2%, 38.95%; higher food allergen was the sea (water) products, among them, shrimp was the highest, about 17.4%. [Conclusion] CVA is the reactive disease that IgE mediated, allergen is the main pathogenic factor. Detection on serum total IgE and allergens specificity IgE has effect in helping diagnosing suspected cough variant asthma disease, l>ut also has significance in the treatment and pre-venlion.%[目的]通过对132例咳嗽变异性哮喘患儿和56例其他慢性咳嗽疾病患儿(对照组)的血清总IgE含量及过敏原特异性IgE的检测,探讨血清总IgE含量及过敏原特异性IgE检测在临床诊治及预防咳嗽变异性哮喘的应用价值.[方法]用美国贝克曼化学发光DX1800检测总IgE含量,使用德国欧蒙印迹法检测20种过敏原特异性IgE.[结果]CVA总IgE阳性率87.1%,特异性IgE阳性率78.8%.对照组总IgE阳性率8.9%,特异性IgE阳性率7.1%.CVA吸入性过敏原阳性率最高,主要是屋尘螨/粉尘螨、霉菌组合、屋尘,分别是55.3%、43.2%、38.95%;食入性过敏原阳性率较高的主要是海(水)产品,其中以虾最高,为17A%.[结论]说明CVA是特异性Ig

  14. Effect of anticancer therapy on Tn antigen exposure on the leucocyte membranes in patients with leukemia

    Directory of Open Access Journals (Sweden)

    G. S. Maslak

    2014-08-01

    Full Text Available Tn-antigen (Thomsen-nouvelle antigen is tumor-associated carbohydrate antigen with only one GalNAc residue attached to serine or threonine of polypeptide chain. There is not enough data about the expression of this glycotope in hematologic processes. But the correlations between increasing Tn-antigen expression on the cell surface and tumor growth progression, invasion, and activation of cell migration are well known. Therefore, the currently important area of modern research is studying of the impact of anticancer therapy by expression of this carbohydrate antigen in the onco-proliferative process. There are two types of cytostatic therapies in clinical hospitals of Ukraine: COP-therapy (cyclophosphamide, vincristine, prednisone and FC-therapy (fludarabine, cyclophosphamide, which are the most popular due to their effectiveness and low price. The aim of our study was to investigate Tn-antigen exposure on the surface of lymphocytes, monocytes and granulocytes in polycythemia vera and subleukemic myelosis; to examine the influence of COP- and FC-therapies on Tn-antigen exponation in patients with chronic lymphocytic leukemia. The objects of the study were blood cells of patients with chronic lymphocytic leukemia (n = 25, polycythemia vera (n = 15 and subleukemic myelosis (n = 15 aged 58–66 years. Healthy hematologic volunteers (n = 15 aged 55 to 65 years were in the control group. Lymphocytes of patients with chronic lymphocytic leukemia (n = 25 were also studied after the chemotherapy treatment of patients divided into two groups: those who took COP-therapy (n = 13; and those who treated with FC-therapy (n = 12. Tn-antigen exposure on lymphocytes, monocytes and granulocytes was investigated by Beckman Сoulter EPICS flow cytometer with primary monoclonal Tn-antigen anybodies (Institute of Immunology, Moscow, Russia and secondary fluorescein isothiocyanate labeled antybodies (Millipore, USA. The number of dead cells was monitored by binding

  15. 匙吻鲟的细胞遗传学分析%CYTOGENETIC ANALYSIS OF POLYODON SPATHULA

    Institute of Scientific and Technical Information of China (English)

    薛淑群; 尹洪滨

    2009-01-01

    Metaphase chromosome specimens of Polyodon spathula, injected with PHA and colchicin, were prepared from short-term culture of kidney cells with air-drying technique. It diploid chromosome number was 2n = 120. The karyotype was composed of 44m, 32sm and 44st, t chromosomes, NF = 196. Diploid nucleus DNA content was measured from the somatic cell of P. spathula, using flow cytometer( Beckman cell Lab Quant, made in USA) and the DNA content (2.3pg/ N) of erythrocytes of chicken ( Gallus domestica) as standard. The DNA content of P. spathula was 6.18pg/N , which was corresponded with the result measured chromosome number as ploidy. According to the present results and date reported, P. spathula was possibly a tetraploid (4n). Combination of the present results and data reported among Acipenseriformes including two families and six genera, Acipenseriformes was the polyploid origin's fish, and was quite completely special at the cell level which evolved by chromosome doubling. The chromosome's doubling and differentiation possibly were primary caused of polyploidization and polyploid type (4n, 8n, 12n and so on) richly. Polyoidization had played an important role in the diversity of Acipenseriformes.%采用体内注射PHA和秋水仙素,肾细胞短期培养,常规空气干燥法制备匙吻鲟(Polyodon spathula)的染色体标本.对其肾细胞染色体数目统计分析表明,匙吻鲟染色体组南120条染色体所组成.以测得的核型参数和按Levan,et al.提出的染色体划分标准得出:具有22对中部着丝粒染色体(m),16对亚中部着丝粒染色体(sm),22对亚端部着丝粒染色体(st)和端部着丝粒染色体(t);染色体臂数(NF)为196,核型公式为44 m + 32am + 44st,t.再采用流式细胞仪分析系统测定匙吻鲟体细胞的DNA含量,与鸡血细胞标准对照相比为2.69±0.19,以鸡红细胞DNA含量2.3 pg/N测算.则匙吻鲟体细胞DNA含量为6.18 pg/N.根据所得到的结果并结合已发表的鲟鱼类DNA含量和

  16. Implications of Climate Volatility for Agricultural Commodity Markets in the Presence of Biofuel Mandates

    Science.gov (United States)

    Verma, M.; Diffenbaugh, N. S.; Hertel, T. W.; Beckman, J.

    2011-12-01

    In presence of bio-fuels, link between energy and agricultural commodity markets has become more complex. An increase in ethanol production to minimum 15bn gallons a year - Renewable Fuel Standard (RFS) and current technically permissible maximum 10% blending limit - Blend Wall (BW); make the link even stronger. If oil prices in future do not rise significantly from their current levels, this minimum production requirement would likely be binding. In such a scenario any fluctuation in crop production will have to be absorbed by the non-ethanol usage of the crop and would translate into crop prices adjusting to clear the markets and therefore the commodity prices will be more volatile. At high oil prices it is possible that the BW may become binding, severing the link between oil prices and commodity prices as well, potentially leading to higher price volatility. Hertel and Beckman (2010) find that, with both RFS and BW simultaneously binding, corn price volatility due to supply side shocks (which could arise from extreme climate events) could be more than 50% as large as in the absence of bio-fuel policies. So energy markets are important determinants of agricultural commodity price volatility. This proposal intends to introduce the increased supply side volatility on account of climate change and volatility, in the framework. Global warming on account of increased GHG concentrations is expected to increase the intensity and frequency of hot extremes in US (Diffenbaugh et al. 2008) and therefore affect corn yields. With supply shocks expected to increase, binding RFS and BW will exacerbate the volatility, while if they are non-binding then the price changes could be cushioned. We propose to model the impacts of climate changes and volatility on commodity prices by linking three main components - a. Projections for change in temperature and precipitation using climate model b. A statistical model to predict impacts of change in climate variable on corn yields in US

  17. "The human race is still a work in progress":An Interview with Richard Powers%"人类仍是一部进步中的作品":理查德·鲍尔斯访谈录

    Institute of Scientific and Technical Information of China (English)

    刁克利

    2007-01-01

    理查德·鲍尔斯是2006年美国国家图书奖小说奖得主,获奖作品《回声制造者》.他于1998年当选美国艺术与科学院院士,现任伊利诺伊大学厄巴纳-香槟分校英语系教授兼贝克曼高级研究院研究员.他屡获殊荣的另外8部小说包括:《我们歌唱的时代》、《冲破黑暗》、《赢利》、《葛拉蒂2.2》、《囚犯的困境》、《三个农民去舞会》等.这次访谈以作家的产生和小说的创作为主要内容,集中讨论了他的作家观和主要创作经历,同时也讨论了他在不同文化中的丰富经历及其对科学技术的广泛探索.在访谈中,他还就好小说给出了他的定义,阐释了作家主要关心的问题和特殊的创作视角,表达了他对文学批评的看法以及他对文学启迪人生意义的信念.本访谈是伊利诺伊大学东亚及太平洋研究中心弗里曼基金项目"创作视角与文学精神:当代美国作家研究"的部分成果.%Richard Powers is the 2006 winner of National Book Award for his fiction The Echo Maker. He was elected fellow of the American Academy of Arts and Sciences in 1998, and he is professor at the Department of English and a full-time member of the Beckman Institute, University of Illinois at Urbana-Champaign(UIUC). He is also author of eight other prize-winning novels:The Time of Our Singing, Plowing the Dark, Gain, Galatea 2.2, Operation Wandering Soul, The Gold Bug Variations, Prisoner's Dilemma and Three Farmers on Their Way to a Dance. This interview concentrates on questions about his idea of being a writer and the making of fiction in general, and also discusses his rich experience of different cultures and his extensive exploration in science and technology. Furthermore, Powers talks about the differences between writing fiction and pursuing science. He gives his definition of good fiction, and explains his main concerns as a writer, his vision of literary criticism, and his belief in

  18. The Argonne Leadership Computing Facility 2010 annual report.

    Energy Technology Data Exchange (ETDEWEB)

    Drugan, C. (LCF)

    2011-05-09

    that will be faster than petascale-class computers by a factor of a thousand. Pete Beckman, who served as the ALCF's Director for the past few years, has been named director of the newly created Exascale Technology and Computing Institute (ETCi). The institute will focus on developing exascale computing to extend scientific discovery and solve critical science and engineering problems. Just as Pete's leadership propelled the ALCF to great success, we know that that ETCi will benefit immensely from his expertise and experience. Without question, the future of supercomputing is certainly in good hands. I would like to thank Pete for all his effort over the past two years, during which he oversaw the establishing of ALCF2, the deployment of the Magellan project, increases in utilization, availability, and number of projects using ALCF1. He managed the rapid growth of ALCF staff and made the facility what it is today. All the staff and users are better for Pete's efforts.

  19. Effect of propofol on glutamate-induced activation and elated inflammatory cytokines of astrocytes from spinal cord dorsal horn

    Institute of Scientific and Technical Information of China (English)

    Chengming Qin; Qing Li; Juying Liu; Tao Zhu; Yong Xiang

    2008-01-01

    BACKGROUND: Astrocytes participate in central nervous system-mediated physiological or pathological processes, such as pain. Activated dorsal horn astrocytes from the spinal cord produce nerve active substances and proinflammatory cytokines, such as interleukin-I beta (IL-1 β ), IL-6, and tumor necrosis factor-a (TNF-a ), which play important roles in pain transduction and regulation. OBJECTIVE: To investigate the effects of different doses of propofol on activation of cultured spinal cord dorsal horn astrocytes induced by glutamate, as well as changes in IL-1 β, IL-6, and TNF-a, and IL-10 (anti-inflammatory cytokine) expression in rats, and to explore the dose relationship of propofnl. DESIGN, TIME AND SETTING: The cellular and molecular biology experiment was performed at the Central Laboratory of Yunyang Medical College between March 2006 and December 2007. MATERIALS: Forty healthy, Wistar rats, aged 2-3 days, were selected. Propofol was provided by Zeneca, UK; glutamate by Sigma, USA; EPICS XL flow cytometry by Beckman culture, USA; rabbit-anti-mouse glial fibrillary acidic protein (GFAP) antibody kit and inflammatory cytokine detection kit were provided by Zhongshan Biotechnology Company Ltd., Beijing; multimedia color pathologic image analysis system was a product of Nikon, Japan. METHODS: Astrocytes were harvested from T11-L6spinal cord dorsal horn of Wistar rats and incubated for 3 weeks. The cells were divided into seven groups, according to various treatment conditions: control group was cells cultured in Hank's buffered saline solution; intralipid group was cells cultured in intralipid (0.2 mL/L); glutamate group was cells cultured with 100 μ mol/L glutamate; propofol group was cells cultured with 250 μ mol/L propofol; three glutamate plus propofol groups were cultured in 100 μ mol/L of glutamate, followed by 5, 25, and 250 μ mol/L of prnpofol 10 minutes later. MAIN OUTCOME MEASURES: GFAP-labeled astrocytes were analyzed using a multimedia

  20. Utility of C-2 (Cyclosporine) monitoring in postrenal transplant patients: A study in the Indian population.

    Science.gov (United States)

    Thakur, V; Kumar, R; Gupta, P N

    2008-07-01

    The study was planned and conducted to assess the benefit of C-2 levels (blood cyclosporine levels two hours postdosing) monitoring over trough (C0) levels (predosing) in postrenal transplant patients. The patient population included 34 postrenal transplant individuals (28 males and six females, mean age of 39.9 +/- 12.3 years). The patients were first-transplant patients and were receiving a microemulsion form of cyclosporine A (CsA) as an immunosuppressant along with azathioprine and prednisolone. In addition, they were not on any enzyme inducer/inhibitor drugs, except for diltiazem. Timed collection of C0 and C-2 samples was done and the samples were immediately processed using the cedia cyclosporine plus assay kit. Estimation was done on a Beckman synchron CX5CE fully automated chemistry analyzer. Serum urea nitrogen and creatinine levels were checked. Poor graft survival was found in this population with 29.3% patients showing graft rejection. The graft rejection patients were assigned to two groups: group I with chronic graft rejection patients (17.6%) and group II with acute graft rejection patients (11.7%). Group III consisted of graft survival patients (70.7%). Mean +/- SD was calculated for C0 and C2 levels. Individual values for C0 and C-2 were plotted on a scatter chart. C0 and C-2 levels were normalized by calculating them as the percentage of their targets (data not shown) and compared using the Kruskal Wallis one-way analysis of variance. C0 levels in all the three groups were within the recommended therapeutic range (150-300 ng/mL) (P < 0.182). Blood C-2 concentrations did not achieve the recommended target levels in these patients. One-way analysis of variance for C-2 values when expressed as the percentage of the target values did not show any significant difference between these groups (P < 0.84). No significant difference was found in C0 levels between groups I, II, and group III patients when expressed as the percentage of the target values (P

  1. Parâmetros solo-máquina em função de doses de resíduos vegetais e profundidades de deposição de adubo em semeadura direta Parameters of soil-machine in function of the amount of cover residues and different fertilizer deposition depths in direct drilling

    Directory of Open Access Journals (Sweden)

    Karina M. Kamimura

    2009-09-01

    Full Text Available O tráfego contínuo e inadequado de máquinas em solos sob semeadura direta tem provocado alterações dos atributos físicos e mecânicos dos solos, influenciando, dessa forma, na produtividade das culturas. O experimento foi conduzido na Estação Experimental Agronômica da Universidade Federal do Rio Grande do Sul, no município de Eldorado do Sul - RS, num Argissolo Vermelho distrófico típico, com o objetivo de quantificar a força de tração e obter informações sobre os atributos físicos do solo, em semeadura direta sobre resíduos de aveia-preta e ervilhaca parcialmente decomposta. O delineamento experimental utilizado foi em blocos casualizados, com parcelas subsubdivididas, sendo, nas parcelas principais, doses de resíduos da cultura de inverno (0; 1,3; 2,6; 3,2; 3,8 e 5,1 Mg ha-1 de palha de aveia-preta consorciada com ervilhaca, nas subparcelas profundidades de atuação da haste sulcadora de adubo (0,06 e 0,12 m e nas subsubparcelas tráfegos dos rodados do trator e colhedora. Os atributos físicos do solo foram afetados pelo tráfego dos rodados da máquina. A força de tração foi influenciada pela profundidade de atuação das hastes sulcadoras e o tráfego dos rodados.The continuous and inadequate machine traffic on soils under direct drilling has been changing the soil physical and mechanical properties, influencing therefore crop productivity. The experiment was carried out at the Agricultural Research Station of the Universidade Federal do Rio Grande do Sul (Eldorado do Sul, RS, Brazil on Typic Paleudlt. The main aim of the work was to measure coulter draft requirements and physical attributes under black oats (Avena strigosa Schieb and common vetch (CV (Vicia sativa L. partially decomposed. The experimental design was carried out in randomized blocks, with split-split-plots and three repetitions. The main treatments were plots with different amount of residues of the winter crop (0, 1,3; 2,6; 3,2; 3,8 and 5,1 Mg ha-1

  2. The subglacial Lake Vostok (East Antarctica) surface snow is Earth-bound DNA (and dust)-free

    Science.gov (United States)

    Bulat, S.; Marie, D.; Bulat, E.; Alekhina, I.; Petit, J.-R.

    2012-09-01

    The objective was to assess the microbial cell abundance in the surface snow in Central East Antarctica and the fate of microbial genomic DNA during summer short-time exposure to surface climatic (and radiation) conditions at Vostok using flow cytometry and DNA-based methods. The surface snow (until 4m deep) was collected as clean as possible in the vicinity of the Vostok station (3 sites - courtesy of A Ekaykin and ASC Lebedev Physical Iinstitute RAS) and towards the Progress station (4 more sites with one just 29km from the coast - courtesy of A Ekaykin and S Popov) in specially decontaminated plastic crates or containers of various volumes (up to 75 kg of snow). All subsequent snow treatment manipulations (melting, concentrating, genomic DNA extraction, primary PCR set up) were performed in clean room laboratory facilities (LGGE, UJF-CNRS, Grenoble, France). Cell concentrations were determined on meltwater aliquots prepared under clean room conditions using flow cytofluorometry (Biostation, Roscoff, France). The highly concentrated meltwater (until 10000 times down) was used to extract gDNA which were subjected to bacterial 16S rRNA genes amplification in PCR and sequencing. The gDNA of a complex mesophile microbial community for exposure trials were also prepared and put onto a filter under strict clean room conditions. The filters were got exposed open to solar radiation and surface temperature at Vostok during January for various time duration periods (from 25 to 1 day). As a result no microbial cells were confidently detected in surface snow samples differed by sampling sites and people asked to collect as well. Complementary the mineral dust particle abundance did not exceed 16 mkg per liter with the particle size mode about 2.5 mkm as shown using Coulter counter. Preliminary amongst the microparticles no unusual findings (e.g. spherules of cosmic origin) were observed by shape and element composition using electron scanning microscopy. The gDNA studies

  3. The clinical application value of the reticulocytes parameters in children anemia%儿童贫血网织红细胞各参数的应用评价

    Institute of Scientific and Technical Information of China (English)

    张俏忻; 肖颖秀; 程碧珍; 林静华

    2011-01-01

    目的 探讨LH750血细胞分析仪检测患儿网织红细胞(Ret)各参数的临床意义.方法 用LH750血细胞分析仪测定120例健康体检儿童及118例贫血患儿的网织红细胞参数.结果 不同病因的贫血中,网织红细胞百分比(RET%)、网织红细胞绝对值(RET#)、未成熟网织红细胞指数(IRF)、强光散射网织红细胞百分比(HLR%)、强光散射网织红细胞绝对值(HLR#)均具有统计学意义(P<0.05).其中溶血性贫血、失血性贫血、肾性贫血、缺铁性贫血、白血病和再生障碍性贫血的HLR%分别为4.02±1.46、2.29±0.87、0.46±0.22、0.50±0.23、0.49±0.25、0.38±0.18;IRF分别为0.56±0.11、0.44±0.13、0.25±0.06、0.26±0.06、0.25±0.05、0.18±0.04.结论 网织红细胞各参数在儿童贫血疾病的诊断及疗效观察中具有较高的应用价值.%Objective To explore the clinical application value of the reticulocytes(RET)parameters in children anemia.Methods RET parameters were analyzed using Coulter LH 750 in 120 healthy children and 118 anemia children with different anemia types .Results There were significant difference (P<0.05) among the different anaemia groups in percentage of RET (RET%) ,RET absolute value (RET # ) ,high light scatter reticulocyte absolute value(HLR # ) ,percentage of high light scater reticulocyte (HLR% ) and pedo- reticulocyte index ( IRF ). The HLR% of hemolytic anemia,hemorrhagic anemia, renal anaemia,iron deficiency anemia,leukemia and aplastic anemia was 4.02±1.46、2.29±0.87、0.46±0.22、0.50±0.23、0.49±0.25 and 0.38±0.18 individually ;and the IRF was 0.56±0.11、0.44±0.13、0.25±0.06、0.26±0.06、0.25±0.05 and 0.18±0.0.Conclusion RET parameters can be of great clinically significance in diagnosis and treatment of chidrent anaemia.

  4. Resistência mecânica do solo e força de tração em hastes sulcadoras de semeadoras-adubadoras em sistema de integração lavoura-pecuária Mechanical resistance of soil and traction force by planter chisel type openers in crop-livestock system

    Directory of Open Access Journals (Sweden)

    Osmar Conte

    2008-12-01

    . Treatments consisted of four grazing intensities, which resulted in pasture heights of 10; 20; 30 and 40 cm, and a no-grazed area used as blank, designed in randomized blocks with three replicates. After the grazing period and during soybean sowing, RP was evaluated; also, required power to openers chisel type coulter was measured. Both parameters increased with increments in grazing intensity. There was correlation between both parameters, showing that it is possible to evaluate the condition of soil´s compaction by measuring draft requirement by fertilizer furrow openers.

  5. 长期低剂量微波辐射对大鼠血清生化和心脏结构的影响研究%Effects of long-term low doses of microwave radiation on biochemical indexes of serum and the myocardial histological structure in rats

    Institute of Scientific and Technical Information of China (English)

    张静; 彭瑞云; 王水明; 高亚兵; 宋薇; 李翔; 董霁; 赵黎

    2011-01-01

    目的 探讨长期低剂量微波辐射致大鼠血清生化、心脏功能及结构的影响.方法 采用2.5、5和10 mW/cm2 微波辐射160只二级雄性Wistar大鼠,5次/周×30 d,6 min/次.于辐射后1 d、14 d、60 d和180 d,采用全自动生化检测仪检测血清钾离子(K+)、钠离子(Na+)、天冬氨酸转氨酶(aspartate aminotransferase,AST)和谷氨酸转氨酶(alanine aminotransferase,ALT)含量;于辐射后6 h、7 d、14 d、30 d和60 d活杀大鼠取心脏,采用光镜和透射电镜观察心脏组织结构及超微结构的改变.结果 2.5、5和10 mW/cm2组大鼠血清Na+含量分别于照射后1 d(P<0.05)和14 d(P<0.01)降低,60 d(P<0.01)和180 d(P<0.05)表达持续升高;AST和ALT含量分别于照射后14 d显著降低(P<0.05);180 d见恢复.2.5、5和10 mW/cm2 微波辐射后7~30 d,大鼠心肌纤维排列紊乱,线粒体肿胀、空化,闰盘结构模糊,此病变随辐射剂量增加呈加重趋势.结论 2.5~10 mW/cm2微波长期辐射可致大鼠K+、Na+、AST及ALT含量异常及心脏组织结构与超微结构损伤.%Objective To investigate the changes of the serum biochemical index and myocardial histological structure in rats after long-term low doses of microwave exposure. Methods One hundred and sixty male Wistar rats were continuously exposed to microwave of 2. 5, 5 and 10 mW/cm2 in a month, 5 times a week and 6 minutes each time. The left ventricle blood was obtained at 1 d, 14 d and 60 d after the rats were irradiated. The K+ . Na+ , AST and ALT level was analysed by Coulter automatic biochemical analyzer. All the experimental rats were sacrificed at 6 h, 7 d, 14 d, 30 d and 60 d after exposure, then the histology and ultrastructure of the heart were observed by light and electron microscope. Results After 2. 5 , 5 and 10 mW/cm2 microwave exposure, heart muscle fibers wavilness , mitochondrial swelling and cavitations,blurred intercalary dics were observed at 7 d - 30 d. Conclusions A long-term of 2. 5 - 10 m

  6. Got Varves?: Reconstructing Holocene Climate Change in Seneca Lake, NY

    Science.gov (United States)

    Rogers, C. E.; Curtin, T. M.

    2004-12-01

    The sedimentary deposits of Seneca Lake, one of eleven Finger Lakes in New York State, contain a valuable record of post-glacial climate and environmental change. Paleoenvironmental interpretations depend on knowing what transport and depositional processes controlled the formation of the laminae. In this study, we examine Holocene rhythmites in two profundal cores collected from the northern half of Seneca Lake to determine possible mechanisms of formation of the alternating olive gray-black layers. Magnetic susceptibility was measured at a 2 cm interval prior to splitting the ˜5 m cores. Split cores were described, photographed, and sampled for loss-on-ignition and grain size analysis at a 10 cm interval. When possible, core one was sampled on a lamination-by-lamination basis whereas core five was sampled at a one cm interval. Sequential LOI was used to estimate the total organic and carbonate content of sediments. Grain size analyses were performed using a Coulter LS 230 laser diffractometer after removal of calcium carbonate. Temporal control of paleoenvironmental changes in cores will be established by two accelerator mass spectrometer radiocarbon dates. The cores contain proglacial pink clay overlain by Holocene mm- to cm-scale alternating olive gray and black laminations of fine-grained sand and mud. Magnetic susceptibility changes are distinct, ranging from 1-22 x 10-6 SI units in core one and 4-57 x 10-6 SI units in core five. Sediment is dominantly composed of siliciclastic material (50-99 weight %) with varying amounts of calcite (0.1-75 weight %) plus lesser amounts of organic matter (0.5-4.1 weight %). A distinct variation in sediment color, organic matter, and carbonate content, and median grain size (φ 50) occurs in the laminated sediment. The olive gray layers are characterized by well-sorted very fine silt and clay with a φ 50 25%), and low organic matter content (1%), lower in carbonate content (6 μ m. Samples are well-sorted and positively

  7. 外周血细胞计数对肝纤维化程度的预测价值%Practical Value of Peripheral Blood Cells Count for Predicting Liver Fibrosis

    Institute of Scientific and Technical Information of China (English)

    张占卿; 陆伟; 童海涛

    2006-01-01

    目的 探讨外周血细胞计数对肝纤维化程度的预测价值.方法 外周血细胞计数采用Coulter Ac·T diff血细胞分析仪检测.用ROC曲线下面积评价外周血细胞计数对S1与S2-4,S1-2与S3-4、S1-3与S4的鉴别价值;用Bayes逐步判别分析,构建判别函数,评价外周血细胞计数对S1、S2、S3、S4的诊断价值.结果 白细胞、血小板、粒细胞计数鉴别S1-2与S3-4的ROC曲线下面积分别为0.339、0.296、0.332,显著小于诊断参考线下面积(P<0.05);红细胞、淋巴细胞、中间细胞计数鉴别S1-2与S3-4的ROC曲线下面积分别为0.414、0.436、0.502,相似于诊断参考线下面积(P>0.05).白细胞、红细胞、血小板、粒细胞、淋巴细胞、中间细胞计数鉴别S1与S2-4和S1-3与S4的ROC曲线下面积分别为0.430和0.546、0.429和0.392、0.547和0.392、0.504和0.437、0.388和0.669、0.415和0.563,均相似于诊断参考线下面积(P>0.05).根据判别函数模型,预测与实测病理分期S1、S2、S3和S4的一致率分别为50.0%、9.5%、61.8%和25.0%,总一致率为43.0%.结论 白细胞、血小板、粒细胞计数鉴别S1-2与S3-4有一定价值;白细胞、红细胞、血小板、粒细胞、淋巴细胞、中间细胞计数鉴别S1与S2-4和S1-3与S4均无价值.判别函数模型鉴别不同病理分期的价值有限.

  8. Desempenho de uma semeadora-adubadora direta, em função da velocidade de eslocamento e do tipo de manejo da biomassa da cultura de cobertura do solo Performance of a seeding-fertilizer machine in function of the displacement speed and soil cover crop biomass management

    Directory of Open Access Journals (Sweden)

    Klinger B. Branquinho

    2004-08-01

    Full Text Available O objetivo do presente trabalho foi estudar o desempenho de uma semeadora-adubadora, operando em duas velocidades sobre a palhada da cultura do milheto (Pennisetum glaucum (L. R. Brown, após diferentes manejos, na semeadura direta da cultura da soja (Glycine Max L.. O experimento foi realizado com seis tratamentos, em esquema fatorial, delineados em blocos ao acaso, com quatro repetições. Os tratamentos combinaram três manejos (triturador de palhas tratorizado, rolo- faca e herbicida com duas velocidades de deslocamento da semeadora-adubadora (5,2 e 7,3 km h-1. Foram analisadas as seguintes variáveis: acúmulo e decomposição de massa seca do milheto, índice de emergência da cultura da soja, uniformidade de distribuição longitudinal de sementes, capacidade de campo efetiva da semeadora-adubadora e produtividade da soja. Os resultados mostraram que não houve diferença na decomposição da massa de milheto após os manejos e que esses, juntamente com a velocidade de deslocamento do conjunto trator-semeadora-adubadora, não influenciaram no número de dias para a emergência das plântulas de soja e na distribuição longitudinal das sementes. A capacidade de campo efetiva da semeadora-adubadora foi maior na velocidade mais alta. O rendimento de grãos não diferiu significativamente nos tratamentos estudados.The objective of the present work was to evaluate three different managements of the Pennisetum glaucum (L. R. Brown crop and two-speed displacement in a no-tillage system of soybean (Glycine max L. crop on a typical Eutrophic Red Latosol. The experiment was arranged in six treatments, in a factorial array, in a randomized complete block design, with four replications. The treatments were constituted from three combination of crop managements (straw chopper, residue coulter and herbicide and two speed displacement of the seeding-fertilizer machine (5.2 and 7.3 km h-1. The following variables have been analyzed: emergency index of

  9. Stem cell harvesting protocol research in autologous transplantation setting: Large volume vs. conventional cytapheresis

    Directory of Open Access Journals (Sweden)

    Balint Bela

    2008-01-01

    Full Text Available Background/Aim. The use of peripheral blood as a source of hematopoietic stem cells (SCs is progressively increasing and has nearly supplanted bone marrow transplantation. Interpatient variability in the degree and kinetics of SC mobilization into peripheral blood is an expected event after conventional chemotherapy-based treatment, followed by sequential administration of recombinant granulocyte-colony- stimulating factor (rHu-CSF. In this study, specific factors associated with the application of two different SC-harvesting approaches, including the use of large volume leukapheresis (LVL vs. repetitive conventional apheresis (RCA, were analyzed. The basic goal of the study was to evaluate the influence of apheresis protocol (collection timing, processed blood volume and cell yield upon the clinical outcome of transplantation. Methods. Results obtained by LVL (76 pts and RCA (20 pts - control group were compared. The SC mobilizing regimen used was cyclophosphamide (4-7 g/m2 or polychemotherapy and rHuG-CSF 10-16 μg/kg of body mess (bm per day. Cell harvesting was performed using COBE-Spectra (Caridian-BCT, USA. The volume of processed blood in LVL setting was ≥ 3.5 - fold of the patient's circulating blood quantity (ranged from 12.7 to 37.8 l. All patients tolerated well the use of intensive treatment, without any side or adverse effects. Our original controlled-rate cryopreservation was carried out with 10% dimethyl sulfoxide (DMSO using Planer R203/200R or Planer 560-16 equipments (Planer Products Ltd, UK. Total nucleated cell (NC and mononuclear cell (MNC counts were examined by flow cytometry (Advia-2120 Bayer, Germany; Technicon H-3 System, USA. The CD34+ cell surface antigen was investigated by the EPICS XL-MCL device (Coulter, Germany. Results. Performing LVL-apheresis, high-level MNC and CD34+ cell yields (7.6±4.6 × 108/kg bm and 11.8±6.5 × 106/kg bm, respectively were obtained. As a result, rapid hematopoietic reconstitution

  10. Effect of Compound Ammonium Glycyrrhizic S combined with Leigongtengduogan tablets in the treatment of eosinophil increases caused by chronic eczema%复方甘草酸单胺S联合雷公藤多甙治疗慢性湿疹引起嗜酸性粒细胞增多疗效观察

    Institute of Scientific and Technical Information of China (English)

    韩雪松; 岳致丰; 周向昭

    2011-01-01

    Objective To explore the effect of Compound Ammonium Glycyrrhizic S combined with Leigongtengduogan tablets in the treatment of eosinophil increases caused by chronic eczema. Methods The ECKMAN COULTER LH750 ANALYZER from the United States was used in analyzing the data. Patients were divided into treatment group with 30 patients applied with Compound Ammonium Glycyrrhizic S combined with Leigongtengduogan tablets and the control group with 30 patients applied with the Citirizine Dihydrochloride Capsules combined with Ketotifen Fumarate Tablets. Before and after the treatment, the blood of eosinophil, self - perceived symptoms, and skin damage changes were recorded and compared. Results After 4- week treatment,the recovery and efficiency rate of the eosinophils decline index in the treatment group were 43.33 %and 96.66 % respectively, which were significantly higher than those in the control group, 3.33 %and 30.00 %. The recovery and efficiency rate of TSS decline index in the treatment group were 37.67 % and 93.34 %, which were significantly higher than those in the control group, 6.67 % and 26.67 %. It is statistically different (P < 0.01). Conclusion Compound Ammonium Glycyrrhizic S combined with Leigongtengduogan tablets can effectively promote eosinophil count drops. Clinical symptoms and physical signs significantly improve.%目的 观察复方甘草酸单胺S联合雷公藤多甙治疗慢性湿疹引起嗜酸性粒细胞增多的临床疗效.方法 采用5分类血细胞分析仪分别对治疗组30例(应用复方甘草酸单胺S联合雷公藤多甙)和对照组30例(应用盐酸西替利嗪胶囊联合富马酸酮替芬)在治疗前后对外周血嗜酸粒细胞计数进行测定,同时记录自觉症状和皮损情况的改善,并对比分析.结果 治疗4周后,治疗组嗜酸性粒细胞下降指数的痊愈率及有效率分别为43.33%、96.66%,明显高于对照组的3.33%、30.00%;治疗组TSS下降指数的痊

  11. 生长激素对胰腺癌IGF-Ⅰ,Ⅱ-IGFBP3通路动态影响的实验研究%Effect of growth hormone on features of IGF-Ⅰ-Ⅱ-IGFBP3 pathway in pancreatic cancer

    Institute of Scientific and Technical Information of China (English)

    石毅; 孙跃明; 白剑锋; 陆文熊; 傅赞; 赵翰林; 苗毅

    2010-01-01

    Objective To investigate the effect of GH on proliferation of pancreatic cancer cells and observe the features of IGF-IGFBP3 pathway in the host after GH administration. Methods Pancreatic cancer cells (SW-1990,PANC-1 and P3) during exponential growth stage were harvested and cultured in medium containing growth hormone (50 ng/ml). After 24, 48 and 72 hours, cells were counted using a Coulter Counter. Thirty-five Athymic nude Balb/c mice were inoculated with SW-1990cells. When tumors became palpable after inoculation, animals were randomized to receive GH points (1 h, 2 h, 6 h, 24 after the last injection), plasma samples were gathered for subsequent ELISA determination and liver was rapidly incised for immune blotting analysis. Results The results revealed that GH stimulated cell growth in vitro. GH elevated levels of IGF-Ⅰ , Ⅱ at the 1st , 2nd , 6th hour after the last injection. GH augmented the expression of IGFBP3 in the liver of the host in vivo (1 h, 2 h, 6 h, 24 h, respectively). Conclusion Such proteins as IGF- Ⅰ and Ⅱ might be associated with mechanism of last effect of GH on tumor host. The up-regulation of IGFBP3 by GH administration in the host may help to explain the phenomena that GH doesn't accelerate growth of pancreatic tumor in vivo.%目的 观察生长激素(GH)对胰腺癌细胞增殖及对肿瘤宿主胰岛素样生长因子Ⅰ,Ⅱ(IGF-Ⅰ,Ⅱ)-胰岛素样生长因子结合蛋白3(IGFBP3)通路的影响,初步探讨体内外实验差异的原因.方法 体外应用GH(50 ng/ml)24 h,48 h,72 h后计数胰腺癌细胞SW-1990,PANC-1及P3;选用BALB/C雌性裸小鼠35只,以SW-1990成瘤,测量瘤体积,成瘤裸鼠随机分为注射GH的实验组(GH组)及对照组(NS组),荷瘤裸鼠在最后依次注射GH后第1小时、2小时、6小时及24小时处死动物,心室穿刺抽血,切除肝脏,酶联免疫吸附法(ELISA)检测血清IGF-Ⅰ,-Ⅱ水平(P<0.05),蛋白印记(Western Blot)方法观察肝脏IGFBP3的

  12. Essai de classement typo-technologique des araires à partir des pièces métalliques découvertes en Gaule romaine en vue de leur reconstitution Setting up of a typological-technological classification method to try to reconstruct ards from metallic pieces found in Roman Gaul

    Directory of Open Access Journals (Sweden)

    André Marbach

    2008-04-01

    positions? A method of research has been developed from a fine analysis of the metallic pieces listed in a catalogue of these pieces for Gaul. The shares have been classified according to their working surface, the form of their sockets and the angle of the wearing surface with the ground if it is observable. As for the tanged shares the filing is similar, the length of the tang has however been taken into account here. From the values known for these pieces and a technical study with modelisation of the tilling implement, the possibility of a reconstruction of the ard has been explored. Researchers are here reminded of how important it is to publish the description of the pieces found with the utmost precision for the drawing as well as for the dimensions. As far as ards fitted with a socket share are concerned uncertainty prevails except for shares with a small working surface which, generally, are single ards (manche-sep. The reconstruction of tanged share-ards seems possible including the length of the draught-beam (chambige. Several concrete examples of reconstructed ards have been studied. Thus, if the reconstruction of a tanged share from metallic pieces, tang and coulter may be envisaged, that of iron share ards is uncertain except for plough-ards (reversible.

  13. Neuronal injury and loss in sAPPα-Tg mice injected with LPS%LPS诱发sAPP-α转基因鼠神经细胞损伤机制的初步探讨

    Institute of Scientific and Technical Information of China (English)

    王政; 韩玉霞; 苏刚; 陈迪祥; 肖元宏

    2012-01-01

    Objective:To research the relationship between infection and autism development . Methods:sAPPα-Tg mice and littermate controls were injected with 50 μg LPS, monitored and weighted everyday until 20 days. The mice were harvested at 12,24, 48,72,96,120 h and 14 w, the blood cell was counted with coulter counter , inflammatory factors were evaluated with ELISA on day 3. The brain sections from sAPPα-Tg mice and littermate controls were reacted with Nissl or NeuN antibody and the stereological analysis was performed for NeuN-positive cells after 14 weeks. Results; Total number of red blood cells and hematocrits decreased in sAPP α-Tg mice compare with littermate control mice at 12,24,48,72 h. The counts of platelets have no obvious change . NeuN immunohistochem-istry disclosed a more rarefied pattern of neurons . Furthermore, Nissl staining revealed neuronal dysmorphology suggestive of neuronal degeneration in sAPPα-Tg mice after 14 weeks. Conclusion: These data show that over-expression of sAPPα on platelets in mice leads to neuronal injury and loss that may be caused by brain hemorrhage .%目的:探讨微生物感染所造成的损伤是否与小儿孤独症的发病相关.方法:我们使用C57BL/6为背景的sAPP-α转基因小鼠和同源对照小鼠,给予低剂量(50 μg)的LPS腹腔注射,于不同时间点12、24、48、72、96、120小时以及14周处死小鼠,收集相应的样本,进行不同时间点外周血细胞计数、72小时 ELISA 方法检测大脑内炎症因子表达水平以及14周大脑免疫细胞化学等检测.结果:实验结果表明,与同源对照小鼠比较,sAPP-α转基因幼鼠在低剂量的LPS作用下,表现为体重减轻,12、24、48、72小时外周血红细胞数和HCT降低、14周大脑海马区存在着广泛的损伤修复区,但外周血血小板计数并无明显改变.对损伤区进行进一步Nissl染色,表明损伤区为不同于任何神经细胞的一种活细胞,推测为出血损伤后的纤维细胞

  14. Red oil A5 inhibits proliferation and induces apoptosis in pancreatic cancer cells

    Institute of Scientific and Technical Information of China (English)

    Mi-Lian Dong; Xian-Zhong Ding; Thomas E. Adrian

    2004-01-01

    AIM: To study the effect of red oil A5 on pancreatic cancer cells and its possible mechanisms.METHODS: Effect of different concentrations of red oil A5on proliferation of three pancreatic cancer cell lines, AsPC-1,MiaPaCa-2 and S2013, was measured by 3H-methyl thymidine incorporation. Time-dependent effects of 1:32 000 red oil A5 on proliferation of three pancreatic cancer cell lines, were also measured by 3H-methyl thymidine incorporation, and Time-course effects of 1:32 000 red oil A5 on cell number.The cells were counted by Z1-Coulter Counter. Fiowcytometric analysis of cellular DNA content in the control and red oil A5 treated AsPC-1, MiaPaCa-2 and S2013 cells,were stained with propidium iodide. TUNEL assay of red oil A5-induced pancreatic cancer cell apoptosis was performed.Western blotting of the cytochrome c protein in AsPC-1,MiaPaCa-2 and S2013 cells treated 24 hours with 1:32 000red oil A5 was performed. Proteins in cytosolic fraction and in mitochondria fraction were extracted. Proteins extracted from each sample were electrophoresed on SDS-PAGE gels and then were transferred to nitrocellulose membranes.Cytochrome c was identified using a monoclonal cytochrome c antibody. Western blotting of the caspase-3 protein in AsPC-1, MliaPaCa-2 and S2013 cells treated with 1:32 000 red oil A5 for 24 hours was carried out. Proteins in whole cellular lysates were electrophoresed on SDS-PAGE gels and then transferred to nitrocellulose membranes. Caspase-3 was identified using a specific antibody. Western blotting of polyADP ribose polymerase (PARP) protein in AsPC-1, MiaPaCa2 and S2013 cells treated with 1:32 000 red oil A5 for 24 hours was performed. Proteins in whole cellular lysates were separated by electrophoresis on SDS-PAGE gels and then transferred to nitrocellulose membranes. PARP was identified by using a monoclonal antibody.RESULTS: Red oil A5 caused dose- and time-dependent inhibition of pancreatic cancer cell proliferation. Propidium iodide DNA staining

  15. Changes of biochemical indexes in patients with acute cerebral infarction after treatment with simvastatin

    Institute of Scientific and Technical Information of China (English)

    Cheng Yang

    2006-01-01

    BACKGROUND: At present, it is believed that the important causes of cerebral infarction are the disorders of lipid metabolism and endothelial function, and the outcomes of clinical treatment can be improved by regulating serum lipids and antiinflammation, etc.OBJECTIVE: To observe the effect of simvastatin, inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, on the levels of serum lipids, serum enzymic indexes and inflammatory metabolic indexes in patients with cerebral infarction.DESIGN : A comparative observation.SETTING: Department of Geriatrics, Longquanshan Hospital of Liuzhou City.PARTICIPANTS: Forty-eight patients with acute cerebral infarction wera selected from the Department of Geriatrics of Longquanshan Hospital of Liuzhou from March 2004 to February 2006, including 24 males and 24females, the mean age was (54±12) years, average disease course was (10.0±4.5) days. They were all accorded with the diagnostic standard for cerebral infarction set by the Fourth National Academic Meeting for Cerebrovascular Disease in 1999, and cerebral hemorrhage was excluded by cranial CT scanning. The 48patients were randomly divided into control group (n =24) and traatment group (n =24). Informed consents were obtained from all the participants.METHODS: ① All the patients wera treated according to the symptoms, besides those in the traatment group were given simvastatin (Harbin Pharm. Group Sanjing Pharmaceutical Shareholding, Co.,Ltd., No. H20010454;Batch number: 20040218; 5 mg/tablet). The initial dosage was 10 mg per day for 4 weeks, and then increased to 30 mg per day for another 4 weeks. ② Before treatment and within 1 week after treatment, the total cholesterol, triglyceride, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST), craatine kinase and C raactive protein in serum were determined with Beckman-cx7 automatic biochemical analytical apparatus in both groups. ③ The

  16. In vitro release of 1,3-bis (2-chloroethyl)-1-nitrosourea sustained-release microspheres and the distribution in rat brain tissues

    Institute of Scientific and Technical Information of China (English)

    Xia Li; Liping Guo; Qin Li

    2006-01-01

    BACKGROUND: The implantation of released chemotherapeutic drugs, which takes biodegradable polymer as vector, into the tumor site can get high concentration and release the drug for a long time, it can directly act on the tumor cells, and reduce the general toxicity.OBJECTIVE: To explore the in vitro and in vivo course of 1,3-bis (2-chloroethyl)-1-nitrosourea (BCNU) sustained-release from BCNU-loaded polylactide (PLA) microspheres (MS) and location in rat brain tissue.DESIGN: A repetitive measurement.SETTING: Central Pharmacy, General Hospital of Chinese People's Armed Police Forces.MATERIALS: Thirty male SD rats were used. PLA (Mr5000, batch number: KSL8377) was produced by Wako Pure Chemical Inc.,Ltd. (Japan); BCNU (batch number: 021121) by Tianjin Jinyao Amino Acid Co., Ltd.;BCNU-PLA-MS was prepared by the method of solvent evaporation and pressed into tablets (10 mg/tablet).High-performance liquid chromatography (HPLC) Agilent 1100 (USA); LS9800 liquid-scintillation radiometric apparatus (Beckman). Chromatographic conditions: Elite Hypersil ODS2 C18 chromatographic column (5 μm,4.6 mm ×150 mm); Mobile phase: methanol: water (50:50), flow rate was 1.0 mL per minute, wave length of ultraviolet detection was 237 nm, and the inlet amount of samples was 10 μL.METHODS: The experiments were carried out in the experimental animal center of the General Hospital of Chinese Armed Police from May 2004 to July 2005. ① In vitro BCNU-PLA-MS release test: BCNU-PLA-MS was prepared by the method of solvent evaporation, then placed in 0.1 mol/L phosphate buffered solution (PBS, pH 7.4, 37 ℃), part of MS were taken out at 1, 2, 3, 7, 10 and 15 days respectively, and the rest amount of BCNU in MS was determined by HPLC, then the curve of BCNU-PLA-MS release was drawn. ②In vivo BCNU-PLA-MS release and distribution test: The rats were anesthetized, then BCNU-PLA-MS were implanted to the site 1 mm inferior to the cortex of frontal lobe. Five rats were killed postoperatively

  17. Klinefelter's综合征伴无精子症患者临床表型及分子细胞遗传学分析%Clinical feature and molecular cytogenetics analysis of Klinefelter's syndrome with azoospermic

    Institute of Scientific and Technical Information of China (English)

    沙艳伟; 宋岳强; 孔辉; 郭奇伟; 林津; 周裕林; 韩斌

    2011-01-01

    patients to understand the illness and to perform the assisted reproductive techniques.%目的:探讨Klinefelter's综合征(Klinefelter's syndrome,KFS)伴无精子症患者临床表型和遗传学特征.方法:收集9例KFS伴无精子症患者和8例正常生育男性组临床资料,精液分析依照世界卫生组织指南操作,外周血淋巴细胞按常规染色体培养及G和Q显带行核型分析,对KFS患者X、Y染色体行荧光原位杂交技术(fluorescence in situ hybridization,FISH)检测确认.Y染色体微缺失选取EAA和EMQN推荐6个Y染色体特异标签序列位点行Y染色体AZFa、AZFb和AZFc区微缺失检查.卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(T)采用BECKMAN ACCESS化学发光法检测.结果:9例患者均以不育就诊,表现为身材高大,喉结不明显,阴毛分布正常或稀少,阴茎正常或短小,除1例伴有Y染色体微缺失者睾丸质地软外,余双侧睾丸质地较硬,睾丸体积<4 ml,精液常规检查离心未见精子,9例患者FSH、LH明显升高,T水平正常值低限或偏低.9例患者染色体核型和FISH检测均为47,XXY,行Y染色体Q带染色均为阳性,其中1例Y染色体偏小FISH示存在缺失,Y染色体微缺失检测显示AZFa、b、c区缺失,其余未检测到缺失,缺失发生率11.11%.8例正常生育男性组染色体核型正常、Y染色体未检测到微缺失.结论:KFS具有典型高促性腺功能低下型性腺功能减退症特点,KFS患者伴有潜在Y染色体微缺失可能,Y染色体微缺失不是KFS患者无精子的主要原因,KFS患者行Y染色体微缺失筛查对于了解病情和行辅助生殖技术是必要的.

  18. Insulin Sensitivity and Islet β Cell Function in Persons with Normal Glucose Tolerance and Different VA/SA Ratio%不同腹内脂肪/皮下脂肪比值的糖耐量正常人群胰岛素敏感性与胰岛β细胞分泌功能的研究

    Institute of Scientific and Technical Information of China (English)

    李萍; 王磊; 刘蓉辉; 宋光耀; 叶蔚

    2011-01-01

    Objective To explore insulin sensitivity and isletβcell function in persons with normal glucose tolerance ( NGT ) and different ratios of visceral adipose tissue area to abdominal subcutaneous adipose tissue area ( VA/SA ). Methods Totally 104 NGT persons, according to the median of VA/SA ratio, were divided as group A ( VA/SA < 0. 4, n = 53 , 20 males and 33 females ) and group B ( VA/SA≥0. 4, n =51, 23 males and 28 females ). All the subjects were given examinations of body mass index ( BMI ), waist circumstance ( WC ), 75 g oral glucose tolerance test ( OGTT ); blood sugar by glucose oxidase method; serum insulin by radioimmunoassay; triglyceride ( TC ), total cholesterol ( TG ), and high density lipoprotein - cholesterol ( HDL - C ) by using Beckman reagents and automatic biochemistry analyzer; VA and SA by using SASIEMENS SOMATOM Sensation 16CT. VA/SA ratio, area under curve of glucose ( AUCglu ), area under curve of insulin ( AUCins ), whole body insulin sensitivity index ( WBISI ) quantitative insulin sensitivity check index ( QUICKI ), and HOMA insulin resistance index ( HOMA - IR ) were calculated for assessment of insulin sensibility; and homeostasis model β - cell function ( HOMA - β ) and insulin secretion index ( △I/ △ G ) for assessment of p - cell function. Results The concentrations of TG, HDL - C, lower density lipoprotein - cholesterol ( LDL - C ), very low density lipoprotein ( VLDL - C ), apolipoprotein A ( ApoA ), and apoli-poprotein B ( ApoB ) were markedly higher in group A than in group B ( P < 0. 01 ); but no statistically significant difference in TG was found between the two groups (P>0. 05). The levels of HOMA - IR, WBISI, QUCKI, AUCglu, and AUCins were significantly higher in group A than in group B ( P <0. 01 ); while the differences in values of HOMA - β and △I/△G between the two groups showed no statistical significance ( P >0. 05 ). The correlations of WBISI, AUCins, and AUCglu with VA/SA ( r = -0. 460, 0

  19. Assessment of the performance of red blood cells folic acid analyzer%红细胞叶酸检测仪性能评价

    Institute of Scientific and Technical Information of China (English)

    杨桂花; 胡梅; 韩静; 张曼

    2013-01-01

    目的通过对国产红细胞叶酸检测仪进行性能分析,以验证其检测能力是否符合行业标准。方法参考美国临床和实验室标准化协会 EP5-A2文件,通过20 d 测定2个水平质控物,获得精密度实验数据,计算批内、批间和实验室内变异系数(CV)并与生物学变异规定的要求相比较;按照EP9-A2文件,通过与Beckman Couter UniCel DxI800全自动免疫分析仪测定结果比对验证仪器的正确度;参照 EP6-A文件,用多项式回归法进行线性评价;通过测定20例能够合理代表健康总体的样本对仪器给定的参考范围进行验证。结果批内CV小于6.67%(1/4生物学变异允许总误差),批间 CV小于8.90%(1/3生物学变异允许总误差),实验室内CV小于13.35%(1/2生物学变异允许总误差);在医学决定水平处预期偏倚的可信区间小于允许的误差范围;二次和三次多项式系数与0比较差异均无统计学意义,检测系统呈线性;厂家给定的参考范围不适合本实验所在地人群。结论国产红细胞叶酸检测仪精密度、正确度和线性符合要求;实验室应建立适合当地人群的参考范围。%Objective Through performance analysis of domestic red blood cells folic acid instrument to verify its testing ability. Methods According to national committee for clinical laboratory standards(NCCLS)EP5-A2,the precision was obtained through two level quality product detection within 20 days then the intra-assay variation coefficient(CV),inter-assay CV and CV in labora-tory was calculated and compared with the precision requirement coming from the red blood cells folic acid biological variation.Ac-cording to EP6-A,the linear evaluation was investigated with method of polynomial regression.By measuring the red blood cells fo-lic acid from 20 persons who presented the total healthy people,the reference scope given by the manufacturer was verified

  20. Preparo do solo em áreas de produção de grãos, silagem e pastejo: efeito na resistência tênsil e friabilidade de agregados Soil tillage in grain and silage-producing areas and pasture: effect on tensile strength and aggregate friability

    Directory of Open Access Journals (Sweden)

    Marina Araújo Bavoso

    2010-02-01

    ção da F. Os resultados demonstraram que a RT foi influenciada pelos sistemas de produção e tipos de preparo do solo. O menor valor de RT foi verificado no solo sob Pastejo, enquanto o Sistema Plantio Direto apresentou o maior valor de RT. A friabilidade não distinguiu os sistemas de produção e tipos de preparo de solo estudados.The understanding and quantification of soil use and management on the soil physical quality are important to develop and select sustainable agricultural systems. The purpose of this study was to evaluate the structural quality of a clayey Brazilian Oxisol under different production and soil tillage systems by measuring the tensile strength (TS and to quantify aggregate friability (F. The study was conducted in Castro, Paraná state, in southern Brazil, using three production systems and seven soil tillage types, in a randomized block design. The following production systems were evaluated: (PS I - ryegrass for soil cover (winter and corn for grain production (summer; (PS II ryegrass for haylage/silage and corn for grain production; and (PS III - ryegrass for grazing and corn for grain production. Seven types of soil tillage were evaluated in each system: (G1 - plow harrow in winter, depth 0.15 m; (G2 - plow harrow in winter and summer, depth 0.15 m; (Plow - disk plow, depth 0.20 m; (Laser wing subsoiler - subsoiler wing tips, to a depth of 0.45 m; (Subsoiler - subsoiler, depth 0.80 m; (Aerator - soil aerater Aeromix®; and (NT - No-tillage, where ryegrass was sown using a double disc and corn with a coulter. From each experimental plot soil blocks (0.20 x 0.15 x 0.07 m were taken which were broken up by hand in their natural aggregates. Then, the aggregates were air-dried for 24 h and passed through sieves with diameters of 12.5 and 19 mm. Forty aggregates from each block were selected and tested for indirect tension to determine TS and quantify F. TS was influenced by the production systems and soil tillage types. The lowest and the

  1. Phase 1 Final Technical Report - MgB2 Synthesis for High Field Performance

    Energy Technology Data Exchange (ETDEWEB)

    Mohit Bhatia; Peter McIntyre

    2009-11-02

    boron results in the formation of parasitic phases such as MgB4, MgB7, etc. Such parasitic phases are a primary element of the connectivity problem, in which even though a sample powder may contain grains of high-quality MgB2, adjacent grains are surrounded by intergrowths of parasitic phases so that current trans-port is badly degraded. The best results to date have been obtained using boron powder produced long ago for a rocket propellant development project. The synthesis process was complex and is now largely lost, and the manufacturing equipment has long since been scrapped. The last batch of the powder has been used during recent years to support MgB2 R&D at several labs, but supplies are dwindling. ATC has identified a first application of its plasma torch to synthesize phase-pure amorphous boron flake using a rapid-quench splat technique. Inexpensive technical-grade boron would be purified of contaminants, then dispersed as an aerosol in inert gas and passed through the plasma torch to melt it into a spray. The spray would be splat-condensed on a rotating drum to form pure amorphous flake. The process would begin with technical-grade boron powder, having good stoichiometric purity, nanoscale particles, but significant contamination of MgO and crystalline boron. We used wet chemistry to remove B2O3 completely and reduced the MgO impurity, and analyzed the particle size distribution using a Coulter counter and the phase composition using X-ray diffrac-tion (XRD). The next step will be to build an rf plasma torch with a recirculating single-component aerosol feed and the cooled splat drum and collector, and undertake process devel-opment for amorphous boron powder. This revised goal has two benefits. First, it is an easier technology than our ultimate goal of a multi-component laminar flow torch. We have been counseled by those experienced in plasma torch technology that our ultimate goal will require a torch that should be feasible but has never been attempted. It

  2. Editorial - [FSRJ]Future Studies Research Journal - V6N2

    Directory of Open Access Journals (Sweden)

    Renata Giovinazzo Spers

    2014-12-01

    éticos do país a partir da maior utilização de gases combustíveis para o aquecimento residencial de água. Aprovado no Fast Track do Semead 2014, o artigo “Quantificação dos planos estratégicos por meio do orçamento empresarial: uma aplicação prática com métodos estocásticos” os autores Marino Luiz Eyerkaufer, Janaina Poffo Possamai, Mirian Buss Conçalves, apresentam neste estudo uma aplicação de métodos estocásticos em que o probabilismo se demonstra como alternativa de minimização das incertezas quanto à assertividade das estimativas. Boa leitura!James Terence Coulter WrightEditorRenata Giovinazzo SpersEditora Adjunta

  3. The Influence of m-Microcyte on Platelet-counting of Hemtology Analyzers of Ftting Curve and Floating Threshold%小红细胞对拟合曲线法与移动浮标法计数血小板结果的影响探讨

    Institute of Scientific and Technical Information of China (English)

    张永为; 王剑超; 汪强; 陆新建; 刘燕

    2011-01-01

    [Objective] To investigate the effect of microcyte of different MCV on the PLT counting using hematology analyzer according to fitting curve and floating threshold in platelet decreased sample. [Methods] All platelet tested patients' wimples, which include 46 sample with MCV (mean corpuscular volume) < 60flL, 77 sample with MCV 60~70fL and 78 sample with MCV 70~80fL, are analyzed separately by Beckinan Coulter LH750 and Sysmex X5-8OOi. The platelet ift counted twice with manual method and then lake mean statistical. Paired t test I? Used to compare the measurement data among groups. {Results] Platelet counts in fitting curve, floating threshold and manual method groups in which the MCV is between 70 and 80 fl., are of no statistical significance; the PLT results in hand work group ore similar with those in fitting curve group when MCV is between 60 and 70 fL.but which are significantly lower than in floating threshold group; if the MCV was less than 60 fL. The PLT results in hand work group are similar with those in fitting curve grvup.bat which are significantly lower than in floating threshold grnup. [ Conclusions ] MCV influence should be concerned white counting PLT with Hematology Analyzers. We should recount the platelet with manual method when MCV ^ 70fL by floating threshold Hematology Analyzer. Fitting curve Hematology Analyzer could avoid the influence of microcyte on platelet-counting.%[目的]探讨不同平均红细胞体积(MCV)参数的小红细胞对拟合曲线法与移动浮标法计数血小板结果的影响.[方法]从日常桂测的患者标本中,选取MCV<60fL的标本42例.MCV在60~70fL的标本63例,MCV在70~80fL的标本89例,分别在采用拟合曲线法的贝克曼LH750和采用移动浮标法的希斯美康XS-800i血细胞分析仪上进行血小板(PLT)计数,并对每份标本行手工显微镜镜检计数二次取均值统计.仪器与手工组间的比较采用配对t检验.[结果]对MCV在70~80fL的标本,拟合曲线

  4. Increasing energy efficiency by geometric modification of hoe-type furrow opener

    Directory of Open Access Journals (Sweden)

    R Rahimzadeh

    2016-09-01

    Full Text Available Introduction Direct planting becomes more common in the recent years, because it conserves soil and water as well as it saves energy and time. However, this technology needs special implements such as seed planter. Given that direct planting is practiced in undisturbed lands, so it was needed to design a special furrow opener. In order to obtain a suitable furrow opener this experiment was conducted in rain-fed Agricultural Research Institute in Maragheh. Materials and Methods Most of seed planters that are used for cultivation in rain fed conditions are equipped by hoe-type furrow opener. Hoe-type furrow openers have good penetration in hard and dry soils. However, they do not have the ability for direct planting. Hoe-type furrow opener was chosen as a model. Then by changing the geometric form of the depth to width ratio (d/w, the two openers were designed. In the first design, which was called O1 two wings and a narrow blade acting as a coulter were added in front of the hoe-type furrow opener. In the second design, which was called O2, in addition to the O1 modification, furrow opener width was decreased and a disk blade was added for seed sowing (Fig. 1. The performance of O1 and O2 openers were compared with the conventional hoe-type furrow opener (check in soil bin and in field conditions. At three different forward speeds (1, 1.5 and 2 m.s-1 with 3 replications, the effects of the openers designs of vertical and horizontal soil forces were evaluated in soil-bin conditions. In order to evaluate the performance of the furrow opener in field conditions, an experiment was conducted using a split plot design based on RCBD at 4 replications. Furrow openers formed the main plots and forward speeds formed the sub plots. Each plot size was 22 meters long in two rows for each treatment. After germination of wheat crop, the numbers of seedlings in two rows were counted (along a one meter. After crop maturity, all plots were harvested by hand and

  5. Editorial - [FSRJ]Future Studies Research Journal - V7N1

    Directory of Open Access Journals (Sweden)

    Renata Giovinazzo Spers

    2015-08-01

    artigo que foi aprovado no Fast Track do III SINGEP. A análise desta pesquisa pode auxiliar na construção do planejamento estratégico de uma pequena empresa em fase de crescimento para que seus objetivos estratégicos sejam atingidos.“Aplicação da Criatividade para Gerar Projetos de Inovação: O Caso Prático de uma Estratégia Didática” também foi aprovado no Fast Track III SINGEP. Os autores: Eliana Maria dos Santos Pereira Alves e Ovídio Felippe Pereira da Silva Júnior discutem elementos como identificação de oportunidades, criatividade, inovação e empreendedorismo, buscando sua inter-relação para compreender como é possível motivar a geração de propostas de projetos inovadores em ambientes de ensino.Os autores Mariana Bassi Sutter, Laura Ferranty Mac Lennan, Gabriela Pelegrini Tiscoski e Edison Fernandes Polo, abordam o tema de brasilidade com o objetivo de compreender as representações da imagem do Brasil e os diversos significados do conceito de brasilidade por diferentes pontos de vista.Competitividade Agressiva é o tema abordado pelo autor Antônio Benedito Oliveira Junior e especificamente, demostra a importância da CA para a sobrevivência e crescimento da empresa em mercados cada vez mais dinâmicos, turbulentos e competitivos.O artigo “A Influência do Nível de Competências e de Maturidade em Gestão de Projetos no Resultado Corporativo de uma Empresa do Setor de Transformação” de autoria de Gislaine Cristina dos Santos Teixeira, Emerson Antônio Maccari e Daniel Simonsen, tem por objetivo analisar a influência das competências e da maturidade em gestão de projetos das equipes matriciais e dos gerentes no desempenho dos projetos e consequentemente no resultado corporativo. Boa leitura! Renata Giovinazzo SpersEditora Científica James Terence Coulter WrightEditor Internacional 

  6. 转染脂联素cDNA对骨骼肌细胞株C2C12肌小管葡萄糖氧化和糖原合成的影响%Effects of transfection with adiponectin cDNA on glycogen synthesis and glucose oxidation in myotubes of skeletal muscle cell strain C2C12

    Institute of Scientific and Technical Information of China (English)

    张淼; 李芳萍; 杨川; 钱艳; 刘丹; 傅祖植

    2007-01-01

    背景:脂联素具有降低血脂、血糖,改善胰岛索敏感性的作用,但脂联素对骨骼肌糖代谢的影响尚无定论.目的:通过转染带小鼠脂联素基因的质粒,观察真核表达的脂联素对骨骼肌细胞株C2C12肌小管葡萄糖氧化和糖原合成的影响.设计:对照实验.单位:中山大学附属第二医院.材料:带小鼠脂联素cDNA的pcDNA3.0质粒,即pcDNA3.0-mad(由Dr.Da-Wei Gong惠赠,University of Maryland),C2C12细胞株(ATCC,CRL-1722),rabbit anti-mouse脂联素 IgG(ACRP303-A,Alpha Diagnostic International),SABC即用型免疫组化试剂盒(博士德),D-[U-14C]葡萄糖(放射比活度9.25~13.32 GBq/mmol,NEC),闪烁液POP,POPOP(Sigma),液体闪烁计数器(LS3801,Beckman,USA).方法:实验于2003-03/08在中山大学附属第二医院中心实验室完成.①pcDNA3.0-mad质粒抽提后采用XhoⅠ和XbaⅠ双酶切,及HindⅢ单酶切鉴定.②用阳离子脂质体介导分别将pcDNA3.0-mad和pcDNA3.0空载质粒转染至C2C12细胞,并通过G418(500 mg/L)筛选3周,收集G418抗性的转染后C2C12细胞,分别建立稳定转染pcDNA3.0-mad和pcDNA3.0的C2C12细胞株.③通过Western blot和免疫组化,鉴定C2C12细胞组、稳定转染pcDNA3.0和pcDNA3.0-mad的C2C12细胞组中有无脂联素蛋白表达.④C2C12肌细胞糖代谢实验分对照组、载体组和pcDNA3.0-mad组3组进行,每组又分别加入0,0.5,5,100 nmol/L胰岛素刺激4个浓度进行观察.在加入含D-[U-14C]葡萄糖的MEM培养液孵育一定时间后,通过液闪测定细胞株C2C12肌小管糖原合成和葡萄糖氧化情况.主要观察指标:稳定转染带小鼠脂联素基因的质粒后,骨骼肌细胞株C2C12肌小管葡萄糖氧化和糖原合成量的变化.结果:①质粒转化及酶切鉴定结果:pcDNA3.0-mad质粒抽提后采用XbaⅠ和XhoⅠ双酶切,及HindⅢ单酶切鉴定.得到的质粒酶切片段与预期相符,脂联素cDNA片段长度为781 bp,质粒片段长度为5 446 bp,脂联素cDNA被插

  7. 职业性接触灭蚁药物对人体免疫功能的影响%Impacts of occupational contact with termite-control drug on human immune function

    Institute of Scientific and Technical Information of China (English)

    刘霞; 朱宝立; 班永宏; 陈敏

    2004-01-01

    BACKGROUND: Many chemical poisons existing in our daily life and environment have immunotoxicity in certain dose including termite-control drug, chlordane, and pesticides containing arsenic. Whether occupational contact with this type of chemical poisons would induce injuries of the immune system of the operators?OBJECTIVE: To investigate the impacts of occupational contact with termite-control drug on the immune functions of the operators.DESIGN: A case-control trial.SETTINGS: Original Department of Occupational Diseases, Toxic and Chemical Laboratory, Jiangsu Hospital of Prevention and Treatment for Occupational Disease.PARTICIPANTS: The study was performed in the original Department of Occupational Diseases, Toxic and Chemical Laboratory, Jiangsu Hospital of Prevention and Treatment for Occupational Disease from January 2002 and December 2003. Contact groups were termite-control group, arsenic-producing group and chlordane-producing group. A total of 216 subjects were selected from the staff who were termite controllers of some termite-control institute, workers produce arsenic drug and chlordane of some pesticide factory. Another 64 healthy subjects who had no occupational in the control group were healthy volunteers had no contact with toxic and harmful substances, other conditions of the control group were similar to that contacts with toxic and harmful substances but other diseases.INTERVENTIONS: ICS apparatus for immune chemistry system made by Beckman Company(USA) and PEG-ultraviolet quantitative method were used for the assay of serum IgA, IgG, IgM, C3, C4, CRP and CIC of operators. The impacts of the length of services, smoking, drinking and the poison concentration in vivo on immune level were analyzed as well.MAIN OUTCOME MEASURES: Serum levels of IgG, IgA, IgM, C3,C4, CRP and CIC.RESULTS: Occupational contacts of termite-control drug significantly reduced serum IgG, IgA and IgM levels( t =2.16 to 2. 35, P < 0. 05); However, there were no significant

  8. 前处理过程对汉江上游谷地“古土壤”粒度测试结果的影响研究%Pretreatment Methods and Their Influences on Grain-size Measurement of Palaeosol in the Upper Reaches of the Hanjiang River Valley, China

    Institute of Scientific and Technical Information of China (English)

    庞奖励; 乔晶; 黄春长; 查小春; 周亚利

    2013-01-01

    对汉江谷地的古土壤用6种方法分别进行了前处理,A处理为样品H2O2和盐酸处理后,烧杯注满水静置72 h后加5 mL分散剂.B处理在A基础上再用超声波再震荡10 min.C在A基础上再用超声波再震荡20min.D处理为H2O2和盐酸处理后,烧杯注满水静置72 h后加10mL分散剂,再用超声波再震荡20 min.E处理为H2O2和盐酸处理后再搅拌15 min,烧杯注满水静置72 h后再加入10mL分散剂,用超声波振荡20 min.F处理为H2O2和盐酸处理后,搅拌30 min,余与E相同.用Beckman公司生产的LS1320型激光粒度仪进行测量.结果表明,样品前处理过程中,超声波震荡及振荡时间是影响粒度测量结果的最主要因素.针对汉江上游谷地的样品而言,类黄土的沉积样品(黄土、表土、河漫滩沉积物等)使用前处理方法A后,颗粒可得到充分的分散,能获得了较好的粒度测量效果.而成壤强烈和黏结性较高的古土壤样品,不同的前处理方法对粒度测量结果影响非常大,平均粒径和众值粒径可出现数倍几倍的变化,其中前处理方法C对古土壤样品较合适,样品颗粒能得到较好的分散,测量效果要好于其它方法(A、B、D、E和F).%The grain size of sediment is an important tool for the extraction of paleo-climate and paleo-environment change information.This article attempts to study the influence of sample pretreatment methods on particle size analysis result.Loess and paleosol sample were chosen from the Qianfangcun profile in the upper reaches of the Hanjiang river valley as the representative material.They are measured using a laser particle analyzer (LS1320) made by Beckman Company,U.S.A.These samples were performed using 6 different pretreatment methods before their grain-size distribution were measured,respectively.The experimental results show that the pretreatment methods and steps for the grain size measurement procedures have certainly influenced on results of grain

  9. Without 'Focus'

    Directory of Open Access Journals (Sweden)

    Aldo Sevi

    2010-12-01

    Full Text Available It is widely accepted that a notion of 'focus', more or less as conceived of in Jackendoff (1972, must be incorporated into our theory of grammar, as a means of accounting for certain observed correlations between prosodic facts and semantic/pragmatic facts. In this paper, we put forth the somewhat radical idea that the time has come to give up this customary view, and eliminate 'focus' from our theory of grammar. We argue that such a move is both economical and fruitful.Research over the years has revealed that the correlations between prosody, 'focus', and the alleged semantic/pragmatic effects of focus are much less clear and systematic than we may have initially hoped. First we argue that this state of affairs detracts significantly from the utility of our notion of 'focus', to the point of calling into question the very motivation for including it in the grammar. Then we look at some of the central data, and show how they might be analyzed without recourse to a notion of 'focus'. We concentrate on (i the effect of pitch accent placement on discourse congruence, and (ii the choice of 'associate' for the so-called 'focus sensitive' adverb only. We argue that our focus-free approach to the data improves empirical coverage, and begins to reveal patterns that have previously been obscured by preconceptions about 'focus'.ReferencesBeaver, D. & Clark, B. 2008. Sense and Sensitivity: How Focus Determines Meaning. Blackwell.Beaver, D., Clark, B., Flemming, E., Jaeger, T. F. & Wolters, M. 2007. ‘When semantics meets phonetics: Acoustical studies of second occurrence focus’. Language 83.2: 245–76.http://dx.doi.org/10.1353/lan.2007.0053Beckman, M. & Hirschberg, J. 1994. ‘The ToBI Annotation Conventions’. Ms.,http://www.cs.columbia.edu/~julia/files/conv.pdf.Bolinger, D. 1972. ‘Accent is predictable (if you are a mind-reader’. Language 48.3: 633–44.http://dx.doi.org/10.2307/412039Büring, D. 2006. ‘Focus projection and default

  10. Browning boreal forests of western North America

    Science.gov (United States)

    Verbyla, David

    2011-12-01

    's boreal forest Remote Sens. 2 2729-47 Riordan B, Verbyla D and McGuire A D 2006 Shrinking ponds in subarctic Alaska based on 1950-2002 remotely sensed images J. Geophys. Res. 111 G04002 Ruess R W, McFarland J M, Trummer L M and Rohrs-Richey J K 2009 Disease-mediated declines in N-fixation inputs by Alnus tenuifolia to early-successional floodplains in interior and south-central Alaska Ecosystems 12 489-502 Stafford J M, Wendler G and Curtis J 2000 Temperature and precipitation of Alaska: 50 year trend analysis Theor. Appl. Climatology 67 33-44 Stow D, Peterson A, Hope A, Engstrom R and Coulter L 2007 Greenness trends of Arctic tundra vegetation in the 1990s: comparison of two NDVI data sets from NOAA AVHRR systems Int. J. Remote Sens. 28 4807-22 van Mantgem P J et al 2009 Widespread increase of tree mortality rates in the western United States Science 323 521-4 Walsh, J E, Chapman W L, Romanovsky V, Christensen J H and Stendel M 2008 Global climate model performance over Alaska and Greenland J. Clim. 21 6156-74 Wendler G and Shulski M 2009 A century of climate change for Fairbanks, Alaska Arctic 62 295-300 Zhang K, Kimball J S, Hogg E H, Zhao M, Oechel W C, Cassano J J and Running S W 2008 Satellite-based model detection of recent climate-driven changes in northern high-latitude vegetation productivity J. Geophys. Res. 113 G03033

  11. Evaluation of VCS technology in diagnosis for acute leukemia and its subtypes%VCS技术在急性白血病及其亚型判断中的应用评价

    Institute of Scientific and Technical Information of China (English)

    金红; 杨肃文; 金春兰; 戴泽宁; 许海红

    2010-01-01

    .273,P均<0.01).AGL各亚型组之间,MNV、MNV-SD、MNC、MNC-SD、MNS-SD值差异有统计学意义(F值分别为21.2、169.5、13.6、3.6、98.6,P<0.01),而MNS值差异无统计学意义(F=2.1,P>0.05).AGL、ALL、AMOL各组散点图分布于相应幼稚细胞区域的比例分别为81%(85/105)、74%(31/42)、74%(23/31).结论 VCS参数能较敏感地反映急性白血病外周血细胞的形态学变化,VCS技术对急性白血病的判断有预测作用.%Objective To evaluate clinical application of VCS technology in diagnosis for acute leukemia and its subtypes by LH750 automated hematology analyzer. Methods A total of 178 leukemia patients (105 acute granilocytic leukemia, 42 acute lymphocytic leukemia, 31 acute monecytic leukemia)and 151 non-leukemia patients (86 granulocytic abnormal cases, 35 lymphocytic abnormal cases,30 monocytic abnormal cases) were enrolled into this study. Peripheral blood samples were analyzed by both Coulter LH750 automated hematology analyzer and manual microscopic examination. Microscopic examination was used as reference to evaluate the diagnosis value of VCS technology in blast detecting. VCS parameters were compared for AGL subsets. The clinical value of VCS technology in diagnosis for different acute leukemia was also evaluated by combining VCS scattergram and suspect flags provided by LH750. Results Compared with manual examination, a sensitivity of 95.5% (170/178) and specificity of 95.4% (144/151) were achieved by VCS technology in detecting blasts in acute leukemia and nonleukemia patients. In patients with AGL, the MNV, MNV-SD, MNC and MNC-SD (224.40 ± 23.37,37.40 ±12. 31,145. 80 ±7. 93,24. 79 ±5. 18) were higher than granulocytic abnormal patients( 169.96 ±11.50,29.21 ± 5.27,133.30 ± 5.50, 10.62 ± 4.09) and the differences were statistically significant ( t values were 16. 832, 5. 148, 5. 735, 19. 953 respectively, P < 0. 01 ). On the contrary, MNS ( 122. 90 ±6. 35) in AGL patients was significantly lower than

  12. only: A Case Study In Projective Meaning

    Directory of Open Access Journals (Sweden)

    Craige Roberts

    2010-12-01

    Full Text Available I offer an integrated theory of the meaning of only in which the prejacent, while not presupposed, is both entailed and backgrounded, hence tends to project (following a general proposal about projection due to Simons et al. 2010. Moreover, I argue, contra Beaver & Brady (2008, that only is not conventionally associated with focus, the focus effects arising instead pragmatically. But I do adopt aspects of their semantics for only, including the presupposition of a pre-order over the elements of its domain.ReferencesAbbott, B. 2000. ‘Presuppositions as non-assertions’. Journal of Pragmatics 32: 1419–37.http://dx.doi.org/10.1016/S0378-2166(9900108-3Abbott, B. 2008. ‘Presuppositions and common ground’. Linguistics and Philosophy 31: 523–538.http://dx.doi.org/10.1007/s10988-008-9048-8Amaral, P. 2010. ‘Entailment, assertion, and textual coherence: the case of almost and barely’. Linguistics 48, no. 3: 525–545.http://dx.doi.org/10.1515/LING.2010.016Amaral, P., Roberts, C. & Allyn Smith, E. 2008. ‘Review of The Logic of Conventional Implicature by Chris Potts’. Linguistics and Philosophy 30: 707–749.http://dx.doi.org/10.1007/s10988-008-9025-2Atlas, J. D. 1993. ‘The importance of being “only”: testing the neo-Gricean versus neoentailment paradigms’. Journal of Semantics 10: 301–18.http://dx.doi.org/10.1093/jos/10.4.301Beaver, D. 2001. Presupposition and Assertion in Dynamic Semantics. CSLI Publications, Stanford, CA.Beaver, D. 2010. ‘Handout from a talk on projective meaning’. Stuttgart, Germany.Beaver, D. & Brady, C. 2003. ‘Always and Only: Why not all focus sensitive operators are alike’. Natural Language Semantics 11: 323–62.http://dx.doi.org/10.1023/A:1025542629721Beaver, D. & Brady, C. 2008. Sense and Sensitivity. Blackwell.http://dx.doi.org/10.1002/9781444304176Beckman, M. E. & Ayers, G. M. 1994. ‘Guidelines for ToBI labeling guide, ver. 2.0’. On-line at The Ohio State University: www