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  1. Beckman-coulter AC.T5diff血液分析仪复检规则的制定与评价%Establishment and Application of the Microscope Review Criteria for the Beckman-coulter AC.T 5diff Hematology Analyzer

    Institute of Scientific and Technical Information of China (English)

    王永锋; 尤涛; 康炜

    2012-01-01

    目的 制定科学合理的血液复检规则并对其进行评价.方法 以国际血液学41条复检规则为参考,结合Beckman-coulter AC.T 5diff血液分析仪的测试性能及报警系统,自定复检规则.对1 400例门诊和住院病人的标本进行全自动血细胞分析并同时涂片镜检,观察细胞数量和形态学变化.比较二者结果,对各条自定复检规则进行统计学分析.结果各条规则中假阳性率最高的是异常淋巴细胞,其次是巨大不成熟细胞及单核细胞.真阳性率最高的是粒细胞相对计数.自定复栓规则形态学评估真阳性率12.3%,假阳性率2.7%,真阴性率83.0%,假阴性率2.0%.结论 制定适合Beck-man-coulter AC.T 5diff血液分析仪的15条复检规则,照此执行,既可保证工作质量又提高工作效率,为临床提供更具参考价值的信息.%Objective To establish the microscope review criteria for the Beckman-coulter AC. T 5diff hematology analyzer. Methods Totally 1 400 blood samples were tested by using the Beckman-coulter AC. T 5diff hematology analyzer and smeared for microscopic examination. True positive rate.false positive rate.true negative rate and false negative rate were e-valuated and calculated according to the 41 rules suggested by the International Consensus Group for hematology review as well as the particular alarm system of the Beckman-coulter AC. T 5diff hematology analyzer. Results The most cases showing false positive rate were abnormal lymphocytes, large immature cells and monocytes. The most case showing true positive rate was relative granulocyte count. Microscopic examination results:true positive rate,false positive rate.true negative rate and false negative rate were respectively 12.3%,2. 7%,83. 0% and 2. 0%, respectively. Conclusion Establishment of reasonable microscope review criteria is beneficial to improving work efficiency, reducing probability of missed diagnosis and providing more useful information for clinical.

  2. Multisite Comparison of CD4 and CD8 T-Lymphocyte Counting by Single- versus Multiple-Platform Methodologies: Evaluation of Beckman Coulter Flow-Count Fluorospheres and the tetraONE System

    OpenAIRE

    Reimann, Keith A.; O'Gorman, Maurice R. G.; Spritzler, John; Wilkening, Cynthia L.; Sabath, Daniel E.; Helm, Karen; Campbell, Donald E.

    2000-01-01

    New analytic methods that permit absolute CD4 and CD8 T-cell determinations to be performed entirely on the flow cytometer have the potential for improving assay precision and accuracy. In a multisite trial, we compared two different single-platform assay methods with a predicate two-color assay in which the absolute lymphocyte count was derived by conventional hematology. A two-color method employing lymphocyte light scatter gating and Beckman Coulter Flow-Count fluorospheres for absolute co...

  3. Multisite Comparison of CD4 and CD8 T-Lymphocyte Counting by Single- versus Multiple-Platform Methodologies: Evaluation of Beckman Coulter Flow-Count Fluorospheres and the tetraONE System

    Science.gov (United States)

    Reimann, Keith A.; O'Gorman, Maurice R. G.; Spritzler, John; Wilkening, Cynthia L.; Sabath, Daniel E.; Helm, Karen; Campbell, Donald E.

    2000-01-01

    New analytic methods that permit absolute CD4 and CD8 T-cell determinations to be performed entirely on the flow cytometer have the potential for improving assay precision and accuracy. In a multisite trial, we compared two different single-platform assay methods with a predicate two-color assay in which the absolute lymphocyte count was derived by conventional hematology. A two-color method employing lymphocyte light scatter gating and Beckman Coulter Flow-Count fluorospheres for absolute counting produced within-laboratory precision equivalent to that of the two-color predicate method, as measured by coefficient of variation of replicate measurements. The fully automated Beckman Coulter tetraONE System four-color assay employing CD45 lymphocyte gating, automated analysis, and absolute counting by fluorospheres resulted in a small but significant improvement in the within-laboratory precision of CD4 and CD8 cell counts and percentages suggesting that the CD45 lymphocyte gating and automated analysis might have contributed to the improved performance. Both the two-color method employing Flow-Count fluorospheres and the four-color tetraONE System provided significant and substantial improvements in between-laboratory precision of absolute counts. In some laboratories, absolute counts obtained by the single-platform methods showed small but consistent differences relative to the predicate method. Comparison of each laboratory's absolute counts with the five-laboratory median value suggested that these differences resulted from a bias in the absolute lymphocyte count obtained from the hematology instrument in some laboratories. These results demonstrate the potential for single-platform assay methods to improve within-laboratory and between-laboratory precision of CD4 and CD8 T-cell determinations compared with conventional assay methods. PMID:10799444

  4. Sensor and control for consistent seed drill coulter depth

    DEFF Research Database (Denmark)

    Kirkegaard Nielsen, Søren; Nørremark, Michael; Green, Ole

    2016-01-01

    The consistent depth placement of seeds is vital for achieving the optimum yield of agricultural crops. In state-of-the-art seeding machines, the depth of drill coulters will vary with changes in soil resistance. This paper presents the retrofitting of an angle sensor to the pivoting point...... of a drill coulter, providing sensor feedback to a control system via an electro hydraulic actuator that delivers an even coulter depth. The results showed a strong correlation between the angle of the coulter and the coulter depth under static (R^2=0.9996) and dynamic (R^2=0.987) operations, verified...... by a sub-millimetre accurate positioning system (iGPS, Nikon Metrology NV, Belgium) mounted on the drill coulter. At a drill coulter depth of 55 mm and controlled by an ordinary fixed spring loaded down force only, the change in soil resistance decreased the mean depth by 23 mm. By dynamically controlling...

  5. Micropipette as Coulter counter for submicron particles

    Science.gov (United States)

    Rudzevich, Yauheni; Ordonez, Tony; Evans, Grant; Chow, Lee

    2011-03-01

    Coulter counter based on micropipette has been around for several decades. Typical commercial Coulter counter has a pore size of 20 μ m, and is designed to detect micron-size blood cells. In recent years, there are a lot of interests in using nanometer pore size Coulter counter to detect single molecule and to sequence DNA. Here we describe a simple nanoparticle counter based on pulled micropipettes with a diameter of 50 -- 500 nm. Borosilicate micropipettes with an initial outer diameter of 1.00 mm and inner diameter of 0.5 mm are used. After pulling, the micropipettes are fire polished and ultrasound cleaned. Chlorinated Ag/AgCl electrodes and 0.1 M of KCl solution are used. The ionic currents are measured using an Axopatch 200B amplifier in the voltage-clamp mode. Several types and sizes of nanoparticles are measured, including plain silica and polystyrene nanospheres. The results will be discussed in terms of pH values of the solution and concentrations of the nanoparticles. Financial support from National Science Foundation (NSF-0901361) is acknowledged.

  6. First Report of Stemonitis splendens Rostaf Causing Bark Decay of Oak Logs Used for Shiitake Cultivation in Korea.

    Science.gov (United States)

    Lee, Jung Han; Kim, Da-Ran; Kwak, Youn-Sig

    2014-09-01

    Severe bark decay disease was observed on oak logs at a shiitake cultivation farm in Geochang-gun, Gyeongnam province. The symptoms observed were fruiting bodies that had developed on the top and side surface of oak logs. As a result, the bark came off easily exposing the sapwood. Slime mold specimens collected from oak logs showed developing fruiting bodies comprising of stalks, hypothallus, capillitium, and columella, and the causal agent of bark decay disease was identified as Stemonitis splendens on the basis of morphological characteristics. To our knowledge, this is the first report of Stemonitis splendens causing bark decay of oak logs used for shiitake mushroom cultivation in Korea. PMID:25346606

  7. Sensor and control for consistent seed drill coulter depth

    DEFF Research Database (Denmark)

    Kirkegaard Nielsen, Søren; Nørremark, Michael; Green, Ole

    The even placement of seeds at the correct depth is crucial for achieving the optimum yield. The depth of drill coulters on state-of-the-art seeding machines is normally set manually by downforce springs or weights will therefore react to different soil resistances. A prototype seeder with one...

  8. Measurements of HbA1c by High Performance Liquid Chromatography in D-10 analyzer and Immunological Method by Beckman Coulter AU480 System: A Comparative Study

    Directory of Open Access Journals (Sweden)

    Md Aminul Haque Khan

    2012-07-01

    Full Text Available Background: HbA1c can be measured by different methods. Enzymatic, boronate affinity chromatographic and cation-exchange high performance liquid chromatographic (HPLC methods are considered as gold standard methods. To make the test cost-effective many laboratories use the immunological method installed in a chemistry analyzer. Objective: To compare the values of HbA1c measured by high performance liquid chromatographic method and immunological method in two laboratory settings. Materials and Methods: This observational and comparative study was conducted in Bangladesh Institute of Health Sciences (BIHS and Enam Medical College, Savar, Dhaka. HbA1c levels measured by HPLC in BIHS were compared with that measured by immunological method in Enam Medical College, Savar, Dhaka. p values 10.0% group, but it was within acceptable limit. Conclusion: HbA1c can be measured by immunological method installed in an automated chemistry analyzer to make the test cost-effective. But HPLC method is preferable.

  9. High-Speed Multipass Coulter Counter with Ultrahigh Resolution.

    Science.gov (United States)

    Edwards, Martin A; German, Sean R; Dick, Jeffrey E; Bard, Allen J; White, Henry S

    2015-12-22

    Coulter counters measure the size of particles in solution by passing them through an orifice and measuring a resistive pulse, i.e., a drop in the ionic current flowing between two electrodes placed on either side of the orifice. The magnitude of the pulse gives information on the size of the particle; however, resolution is limited by variability in the path of the translocation, due to the Brownian motion of the particle. We present a simple yet powerful modified Coulter counter that uses programmable data acquisition hardware to switch the voltage after sensing the resistive pulse of a nanoparticle passing through the orifice of a nanopipet. Switching the voltage reverses the direction of the driving force on the particle and, when this detect-switch cycle is repeated, allows us to pass an individual nanoparticle through the orifice thousands of times. By measuring individual particles more than 100 times per second we rapidly determine the distribution of the resistive pulses for each particle, which allows us to accurately determine the mean pulse amplitude and deliver considerably improved size resolution over a conventional Coulter counter. We show that single polystyrene nanoparticles can be shuttled back and forth and monitored for minutes, leading to a precisely determined mean blocking current equating to sub-angstrom size resolution. PMID:26549738

  10. Laboratory evaluation of the Beckman Synchron CX3 clinical chemistry analyzer.

    Science.gov (United States)

    Peake, M J; Pejakovic, M; White, G H

    1988-02-01

    In this evaluation of the Beckman Synchron CX3, the multi-analyte clinical chemistry analyzer exhibited high precision, good linearity, and no carryover for each of the eight analytes measured. Results obtained correlated well with those produced by our routine instrumentation (Beckman Astra, Varian atomic absorption spectrophotometer). The instrument can process up to 75 samples per hour (600 tests per hour if all tests available are requested) and, after calibration, can provide urgent results for the complete panel of tests within 2 1/2 min. The performance characteristics of this instrument make it ideal as a routine or a "stat" analyzer for commonly requested tests in the clinical chemistry laboratory.

  11. The Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin.

    LENUS (Irish Health Repository)

    Byrne, Brendan

    2010-02-01

    Commercially available prolactin immunoassays detect macroprolactin to variable degrees. Best practice requires laboratories to assess the cross-reactivity of their prolactin assay with macroprolactin, and where appropriate, introduce a screen for the presence of macroprolactin. Our policy has been to reanalyse hyperprolactinaemic samples following polyethylene glycol (PEG) precipitation and to report the resultant value as the monomeric prolactin content of the sample. The goal of this study was to determine the need to continue PEG precipitation when prolactin measurements with the Wallac AutoDELFIA were replaced by the Beckman DxI 800.

  12. Evaluation of the Beckman Synchron CX4 clinical chemistry analyzer in a hospital laboratory.

    Science.gov (United States)

    Ambus, T; Korogyi, N; DeCampos, F; Groom, B; Innanen, V T

    1990-01-01

    The Beckman Synchron CX4 random-access multianalyzer was evaluated in a medium sized hospital laboratory. The instrument does end-point, rate, and multipoint assays and carries on-board reagents for 24 tests. In addition to predefined tests, the instrument can be programmed for 100 user-defined tests; these are stored on the hard disk and can utilize up to three component reagents each. The throughput is 200 tests per hour. There is stat testing capability. In our evaluation, within-run and between-run precision and linearity were good, and no reagent carryover was detected. There was good correlation with the in-house methodology for the 19 tests evaluated. A disadvantage at the time of evaluation was interference by elevated bilirubin on creatinine, phosphorus, uric acid, and triglycerides. This problem of interference is being addressed by the manufacturer.

  13. "Comments on Coulter and Smith": The Issue of Authorial Surplus in Narrative Research

    Science.gov (United States)

    Smith, Michael W.

    2009-01-01

    Advocates of narrative research often contend that it is superior to nonnarrative research, both qualitative and quantitative, because they believe it is better able to represent multiple perspectives and foster multiple interpretations. The author of this comment article on Coulter and Smith (2009) draws on literary theory and an analysis of the…

  14. Conformal transformation for the Coulter--Weinberg form of the equations for mass zero spin-2 field

    Energy Technology Data Exchange (ETDEWEB)

    Galles, C.D.; Zandron, O.S.

    1976-05-01

    In this work we show that it is impossible to introduce a third-rank tensor potential that preserves the conformal covariance of the mass zero spin-2 field equations in the Coulter--Weinberg scheme. (AIP)

  15. CMOS-Compatible Silicon-Nanowire-Based Coulter Counter for Cell Enumeration.

    Science.gov (United States)

    Chen, Yu; Guo, Jinhong; Muhammad, Hamidullah; Kang, Yuejun; Ary, Sunil K

    2016-02-01

    A silicon-nanowire-based Coulter counter has been designed and fabricated for particle/cell enumeration. The silicon nanowire was fabricated in a fully complementary metal-oxide-semiconductor (CMOS)-compatible process and used as a field effect transistor (FET) device. The Coulter counter device worked on the principle of potential change detection introduced by the passing of microparticles/cells through a sensing channel. Device uniformity was confirmed by scanning electron microscopy and transmission electron microscopy. Current-voltage measurement showed the high sensitivity of the nanowire FET device to the surface potential change. The results revealed that the silicon-nanowire-based Coulter counter can differentiate polystyrene beads with diameters of 8 and 15 μm. Michigan Cancer Foundation-7 (MCF-7) cells have been successfully counted to validate the device. A fully CMOS-compatible fabrication process can help the device integration and facilitate the development of sensor arrays for high throughput application. With appropriate sample preparation steps, it is also possible to expand the work to applications such as rare-cells detection.

  16. CMOS-Compatible Silicon-Nanowire-Based Coulter Counter for Cell Enumeration.

    Science.gov (United States)

    Chen, Yu; Guo, Jinhong; Muhammad, Hamidullah; Kang, Yuejun; Ary, Sunil K

    2016-02-01

    A silicon-nanowire-based Coulter counter has been designed and fabricated for particle/cell enumeration. The silicon nanowire was fabricated in a fully complementary metal-oxide-semiconductor (CMOS)-compatible process and used as a field effect transistor (FET) device. The Coulter counter device worked on the principle of potential change detection introduced by the passing of microparticles/cells through a sensing channel. Device uniformity was confirmed by scanning electron microscopy and transmission electron microscopy. Current-voltage measurement showed the high sensitivity of the nanowire FET device to the surface potential change. The results revealed that the silicon-nanowire-based Coulter counter can differentiate polystyrene beads with diameters of 8 and 15 μm. Michigan Cancer Foundation-7 (MCF-7) cells have been successfully counted to validate the device. A fully CMOS-compatible fabrication process can help the device integration and facilitate the development of sensor arrays for high throughput application. With appropriate sample preparation steps, it is also possible to expand the work to applications such as rare-cells detection. PMID:26799578

  17. Observation and analysis of the Coulter effect through carbon nanotube and graphene nanopores.

    Science.gov (United States)

    Agrawal, Kumar Varoon; Drahushuk, Lee W; Strano, Michael S

    2016-02-13

    Carbon nanotubes (CNTs) and graphene are the rolled and flat analogues of graphitic carbon, respectively, with hexagonal crystalline lattices, and show exceptional molecular transport properties. The empirical study of a single isolated nanopore requires, as evidence, the observation of stochastic, telegraphic noise from a blocking molecule commensurate in size with the pore. This standard is used ubiquitously in patch clamp studies of single, isolated biological ion channels and a wide range of inorganic, synthetic nanopores. In this work, we show that observation and study of stochastic fluctuations for carbon nanopores, both CNTs and graphene-based, enable precision characterization of pore properties that is otherwise unattainable. In the case of voltage clamp measurements of long (0.5-1 mm) CNTs between 0.9 and 2.2 nm in diameter, Coulter blocking of cationic species reveals the complex structuring of the fluid phase for confined water in this diameter range. In the case of graphene, we have pioneered the study and the analysis of stochastic fluctuations in gas transport from a pressurized, graphene-covered micro-well compartment that reveal switching between different values of the membrane permeance attributed to chemical rearrangements of individual graphene pores. This analysis remains the only way to study such single isolated graphene nanopores under these realistic transport conditions of pore rearrangements, in keeping with the thesis of this work. In summary, observation and analysis of Coulter blocking or stochastic fluctuations of permeating flux is an invaluable tool to understand graphene and graphitic nanopores including CNTs. PMID:26712649

  18. Observation and analysis of the Coulter effect through carbon nanotube and graphene nanopores.

    Science.gov (United States)

    Agrawal, Kumar Varoon; Drahushuk, Lee W; Strano, Michael S

    2016-02-13

    Carbon nanotubes (CNTs) and graphene are the rolled and flat analogues of graphitic carbon, respectively, with hexagonal crystalline lattices, and show exceptional molecular transport properties. The empirical study of a single isolated nanopore requires, as evidence, the observation of stochastic, telegraphic noise from a blocking molecule commensurate in size with the pore. This standard is used ubiquitously in patch clamp studies of single, isolated biological ion channels and a wide range of inorganic, synthetic nanopores. In this work, we show that observation and study of stochastic fluctuations for carbon nanopores, both CNTs and graphene-based, enable precision characterization of pore properties that is otherwise unattainable. In the case of voltage clamp measurements of long (0.5-1 mm) CNTs between 0.9 and 2.2 nm in diameter, Coulter blocking of cationic species reveals the complex structuring of the fluid phase for confined water in this diameter range. In the case of graphene, we have pioneered the study and the analysis of stochastic fluctuations in gas transport from a pressurized, graphene-covered micro-well compartment that reveal switching between different values of the membrane permeance attributed to chemical rearrangements of individual graphene pores. This analysis remains the only way to study such single isolated graphene nanopores under these realistic transport conditions of pore rearrangements, in keeping with the thesis of this work. In summary, observation and analysis of Coulter blocking or stochastic fluctuations of permeating flux is an invaluable tool to understand graphene and graphitic nanopores including CNTs.

  19. Label-free protein detection using a microfluidic Coulter-counter device

    DEFF Research Database (Denmark)

    Rodriguez-Trujíllo, Romén; Ajine, Mohammad Akram; Orzan, A.;

    2014-01-01

    A new method for measuring specific protein concentrations in solutions has been developed. The technique makes use of the Coulter effect for detecting and sizing of micro-scaled objects suspended in a buffer fluid. The method is completely label-free as it is only based on the electrical readout...... rat IgG in solution. When the analyte (rat IgG) is present oligomers of beads are formed. The electrical readout of the oligomers is different compared to a zero control sample with no rat IgG. Detection of the protein has been performed in a concentration as small as 14 ng/mL. The dynamic range...... when a suspension of microscopic beads flows over a set of electrodes in a microfluidic device. Since no electrode functionalization is needed the same device can be used in a number of different assays. Using goat-anti-rat IgG functionalized polystyrene beads we have shown proof of principle detecting...

  20. Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry.

    Science.gov (United States)

    Pasquier, Jennifer; Rioult, Damien; Abu-Kaoud, Nadine; Hoarau-Véchot, Jessica; Marin, Matthieu; Le Foll, Frank

    2015-06-24

    The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD) where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp). The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading), we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.

  1. Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry

    Directory of Open Access Journals (Sweden)

    Jennifer Pasquier

    2015-06-01

    Full Text Available The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp. The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading, we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.

  2. Laser supported particle detecting system in comparison to the Coulter Counter and the Hiac PA 720

    Energy Technology Data Exchange (ETDEWEB)

    Doell, B.; Hoffmann, E.; Lawler, D.

    1986-08-01

    The purpose of these investigations is to demonstrate the ability of a standard Laser-Doppler-Velocity-Equipment (LDV) to get not only information about local velocities and their fluctuations but also about local size and concentration of particles suspended in flowing medias. Such information are of main interest not only for fundamental research but also for particle (size) applications. Particle detecting systems are commonly used in various fields of interest, for example, in indicating the particular pollution of air, water or chemical agents. In water technologies, particle-analysing systems are especially applied to quantify the efficiency of processing units (e.g. filtration or sedimentation steps). Starting with the method of Chigier and co-workers (1979) some experiments were run to get similar information in fluid flow carrying particles. To test the quality of the laser results, simultaneous measurements were performed using standard particle analysing systems. The systems were the Coulter Counter and the Hiac PA 720. A detailed comparison of both was the object of an extra investigation (Lawler, 1984).

  3. Measurement of the volume growth rate of single budding yeast with the MOSFET-based microfluidic Coulter counter.

    Science.gov (United States)

    Sun, Jiashu; Stowers, Chris C; Boczko, Erik M; Li, Deyu

    2010-11-01

    We report on measurements of the volume growth rate of ten individual budding yeast cells using a recently developed MOSFET-based microfluidic Coulter counter. The MOSFET-based microfluidic Coulter counter is very sensitive, provides signals that are immune from the baseline drift, and can work with cell culture media of complex composition. These desirable features allow us to directly measure the volume growth rate of single cells of Saccharomyces cerevisiae LYH3865 strain budding yeast in YNB culture media over a whole cell cycle. Results indicate that all budding yeast follow a sigmoid volume growth profile with reduced growth rates at the initial stage before the bud emerges and the final stage after the daughter gets mature. Analysis of the data indicates that even though all piecewise linear, Gomperitz, and Hill's function models can fit the global growth profile equally well, the data strongly support local exponential growth phenomenon. Accurate volume growth measurements are important for applications in systems biology where quantitative parameters are required for modeling and simulation. PMID:20717618

  4. 甘肃省马铃薯束梗褐腐病病原鉴定及其生物学特性研究%Identification and biological characteristics of Solanum tuberosum brown rot caused by Doratomyces stemonitis

    Institute of Scientific and Technical Information of China (English)

    陈泰祥; 陈秀蓉; 杨成德; 朱海波; 王涵琦; 卞静

    2014-01-01

    The identification and biological characteristics of the pathogen isolated from brown rot of Solanum tu-berosum were studied.The pathogen was identified as Doratomyces stemonitis (Pers.ex Fr.)F.J.Morton &G. Smith based on morphological characteristics as well as rDNA-ITS sequences.The optimum temperature for myce-lial growth was 25-30 ℃ at pH 6.The pathogen could use monosaccharide,disaccharide and polysaccharide;the best carbon source was sucrose,and the best nitrogen source was glycine.Illumination had no obvious promotion effect on mycelial growth.The optimum temperature for sporangium sprout was 25 ℃ at pH 7.Spores could ger-minate only in water drop.The filtrate of potato and glucose solution had obvious promotion effect on spore ger-mination.Soil solution had no effects on spore germination.%对甘肃省马铃薯(Solanum tuberosum L.)束梗褐腐病病原进行了分离鉴定和生物学特性研究。经形态特征观察与 rDNA-ITS 序列分析,将病原菌鉴定为细基束梗霉[Doratomyces stemonitis (Pers.ex Fr.)F.J.Morton &G.Smith]。生物学特性研究结果表明,病菌菌丝生长最适温度为25~30℃,最适 pH 为6,病菌能利用多种碳源,但以蔗糖最好,氮源以甘氨酸最适,光照对菌丝生长没有影响。分生孢子在5~40℃范围内均能萌发,最适25℃,最适 pH 为7,分生孢子萌发需液态水,湿度低于99%几乎不萌发,马铃薯汁液和葡萄糖液对孢子萌发有较好的促进作用。本研究为马铃薯束梗褐腐病的防治提供了理论依据。

  5. 免耕播种机圆盘破茬刀工作性能分析与试验%Analysis and experiment on working performance of disc coulter for no-tillage seeder

    Institute of Scientific and Technical Information of China (English)

    白晓虎; 林静; 吕长义; 胡艳清

    2014-01-01

    Compared with traditional tillage, conservational tillage-also known as zero tillage can effectively prevent soil from wind and water erosion, resist spring drought, prevent sandstorm, increase crop yield, and save cost. No-tillage planter is an important agricultural implement for conservational tillage. In northeast China where ridge culture is the primary planting mode for maize production, the highly effective stubble-cutting device is required because the soil in this region is often covered by hard stubble and a small amount of stalks. Stubble-cutting device is one of the key components affecting performance of no-tillage planter. Two kinds of stubble-cutting device are commonly used, including power-driven device and passive one. Power-driven device can smash stubble and create good seedbed, but is also associated with soil disturbance, high power consumption and blade wear. Passive device of simple structures consumes less energy and only causes slight soil disturbance. Among them, rolling types of disc stubble-cutting coulters can easily cut through stubble under the soil surface without throwing stubbles out of the soil, so it is suitable for application in Northeast China. There are few studies on cutting velocity and working resistance of dis coulters. However, an understanding of cutting velocity and working resistance of dis coulters is necessary for design and application of dis coulters. Therefore, this study investigated the cutting velocity under the condition that coulters slides occurred, and working resistance in vertical direction as well as tractive resistance by kinematics and force analysis, the influence of auxiliary weight and unit working speed on stubble-cutting depth of three disc coulters (plain, wavy, and turbo) in single factor field experiments, and the influence of disc coulter type, auxiliary weight and unit working speed on tractor resistance in an orthogonal field experiment of three factors and three levels. Both theoretical

  6. 应用NCCLS文件对Beckman CX9全自动生化分析仪的精密度进行研究

    Institute of Scientific and Technical Information of China (English)

    莫凡

    2005-01-01

    目的对已使用了5年的Beckman CX9全自动生化仪进行精密度评价.方法通过测定K+、ALT、ALB、TG的含量,评价其批内精密度(Swr)、总精密度(ST).结果K+、ALT、ALB、TG高低浓度的Swr分别为0.23mmol/L、0.10mmol/L;1.82IU/L、0.90IU/L;1.94g/L、1.31g/;0.06mmol/L、0.02mmol/L;Sr分别为0.26 mol/L、0.12 mmol/L;1.62 IU/L、1.28 IU/L;1.77 g/L、1.23 g/L;0.08 mmol/L、0.03mmol/L;x2检验x2<x2(95%)P>0.05.结论该仪器使用5年后精密度依然良好.

  7. Microchip Coulter particle counter

    DEFF Research Database (Denmark)

    Larsen, Ulrik Darling; Blankenstein, Gert; Branebjerg, J.

    1997-01-01

    operation. By sheathing of a nonconductive liquid on either side of an electrolyte, it is possible to optimize the sensitivity to a specific cell-size. A method for measuring the relative flow-rates between liquid phases with different conductivity is presented. The method utilizes the laminar flow...... and short contact time of liquids in microchannels. As a result, the width of the liquids can be controlled without knowing the actual flow rates. The μCPC has been fabricated by standard microfabrication techniques, including RIE, wet silicon etching, metalization and anodic bonding...

  8. Evaluation of self-established assay system in Beckman DXC800 chemistry analyzer measured by prealbumin reagent kits%应用国产前白蛋白在Beckman上自建检测系统及其评价

    Institute of Scientific and Technical Information of China (English)

    张家云; 邓芳; 孙永梅; 李明

    2013-01-01

    目的:应用EP9-A对某国产前白蛋白在Beckman上的自建检测系统,并进行评价和偏差评估。方法:依据美国临床实验室标准化委员会(NCCLS)EP9-A应用北京利德曼前白蛋白(PA)试剂盒及其配套校准品、质控品在Beckman DXC800全自动生化分析仪的检测系统对40份患者新鲜血清的PA进行检测,并与Beckman公司原装配套的前白蛋白试剂盒(PAB)比对,对检测浓度进行系统评价和偏差分析。结果:北京利德曼前白蛋白(PA)试剂在Beckman DXC800全自动生化分析仪上的准确度、批内精密度、线性均符合要求,两种试剂检测结果无显著差异(P>0.05),具有较好的可比性,其相关系数(r2)>0.99。结论:自建检测系统血清PA检测结果与Beckman可溯源的参考检测系统相比,可被临床接受。%Objective:To evaluation the comparability and clinical acceptability of national prealbumin reagent kits measured by the self-established assay system in Beckman DXC800 chemistry analyzer.Methods:The levels of prealbumin in 40 patients' fresh serum, Calibrators and Controls were analyzed by self-established and Beckman ref-erence assay system. To compared with each other, the bias were estimated according to National Committee for Clini-cal Laboratory Standards (NCCLS) document EP9A2 files. Results:The trueness, inter-precision and linear of Beijing Leadman prealbumin reagent kits measured by the self-established assay system in Beckman DXC800 chemistry an-alyzer were acceptable. There was no statistically significant difference, P>0.05. The correlation coefficient (r2) was more than 0.99. Conclusion:The comparability of national prealbumin measured by the self-established assay system in Beckman DXC800 chemistry analyzer compared with Beckman reference assay system was acceptable.

  9. Analysis of the correlation of the results obtained by Vitros350 dry chemistry analyzer and Beckman AU5800 automatic biochemical analyzer%Vitros350干化学分析仪和Beckman AU5800全自动生化分析仪检测结果相关性分析

    Institute of Scientific and Technical Information of China (English)

    谢光素; 周琳; 陈华根

    2015-01-01

    ObjectiveAnalysis of the correlation of the results obtained by Vitros350 dry chemistry analyzer and Beckman AU5800 automatic biochemical analyzer.MethodsA random collection of 40 cases of hospitalized patients with qualified serum specimens,respectively with Vitros350 dry chemistry analyzer and Beckman AU5800 automatic biochemical analyzer analysis of total bilirubin,alanine aminotransferase,aspartate aminotransferase,total protein,albumin,gamma glutamyl transpeptidase,alkaline phosphatase,urea nitrogen,creatinine,uric acid,creatine kinase,creatine kinase isoenzyme, calcium,phosphorus,magnesium,carbon dioxide,amylase,lipase and glucose,a total of 19 projects,the results were statistically analyzed.ResultsExcept nine projects such as alanine aminotransferase,aspartate aminotransferase,albumin, urea nitrogen,creatinine,uric acid,amylase,lipase and glucose test results there was no statistically significant difference (P>0.5),statistically significant difference were observed in the rest of the project,relevant regression analysis showed that total bilirubin,total protein,gamma glutamyl transpeptidase and alkaline phosphatase linear good(r>0.975),creatine kinase, creatine kinase isoenzyme,magnesium,calcium,phosphorus,carbon dioxide,must expand the scope of valid data(r0.5),其余项目差异均具有统计学意义。相关性分析显示总胆红素、总蛋白、谷氨酰转肽酶、碱性磷酸酶线性关系良好(r>0.975),肌酸激酶、肌酸激酶同工酶、钙、无机磷、镁、二氧化碳必须扩大有效数据范围(r<0.975)。结论不同检测系统的仪器检测同一项目时,应进行相关性分析,对相关性较差的项目须立即采取措施,使结果具有可比性。

  10. Enough room for Williams and IMF? / Paul Beckman

    Index Scriptorium Estoniae

    Beckman, Paul

    1999-01-01

    Lõppesid Leedu ja USA energeetikakompanii Williams International läbirääkimised Leedu naftakompleksis osaluse omandamise asjus. IMF uurib Leedu majanduslikku arengut, mida tehing Williamsiga komplitseerib

  11. Analytical validation of serum bone alkaline phosphatase (BAP OSTASE) on Liaison

    OpenAIRE

    Cavalier, Etienne; Rozet, Eric; Carlisi, Agnès; Bekaert, Anne-Catherine; ROUSSELLE, Olivier; Hubert, Philippe; Chapelle, Jean-Paul; Delanaye, Pierre

    2009-01-01

    Background: The goal of this study was to validate the DiaSorin Liaison BAP OSTASE, a new method for measurement of bone alkaline phosphatase (BAP), and to compare this method with the Beckman-Coulter Access Ostase. We also wanted to establish the reference range for BAP in adults and children. Methods: We determined the precision, functional sensitivity, recovery, linearity and measurement uncertainty, accuracy profile and β-expectation limits. We defined an adult reference interval using i...

  12. Força de tração e volume de solo mobilizado por haste sulcadora em semeadura direta sobre campo nativo, em função do teor de água no solo, profundidade e velocidade de operação Draft and soil loosening by knife type coulter related to soil moisture and planter's working speed and depth

    Directory of Open Access Journals (Sweden)

    Carla T. C. Cepik

    2005-08-01

    's coulters. The specific aims of the present work are to determine the knowledge of coulter draft requirements and the amount of displaced soil and tractor slippage on a Paleudult soil at different soil moisture condition, in direct planting on natural pastures chemically killed. Draft requirements increased with an increase in working depth. The increase of the ground speed had no effect on draft when soil was dry or moist; it increased however, on friable soil. Soil displacement was always higher with dry soil and at 12 cm working depth. When soil was friable or dry, the front wheels traction was needed to keep tractor slippage within acceptable levels.

  13. [Estimation of mescaline and pellotine in Lophophora coulter plants (Cactaceae) by means of the oscillographic polarography].

    Science.gov (United States)

    Gabermann, V

    1978-02-01

    Oscillographic polarography has been applied for the mescaline and pellotine estimation. These alkaloids produce in 0.5 N NaOH electrolyte a sharp peak within the cathode region of the oscillogram, each of them showing different potential. It makes possible to estimate them at a concentration of 5.10(-6) g/ml. All the forms of Lophophora williamsii were found to contain mescaline and lower content of pellothine, L. jourdaniana--to have equal content of both alkaloide, L. diffusa and L. fricii--to contain pellotine and only traces of mescaline. Plants grown in the greenhouse accumulated the same amount of alkaloids as native plants. Grafting on roodstock which does not produce essential amount of the alkaloids, does not affect the ability of Lophophora to synthesize mescaline and pellotine.

  14. APPLICATION OF SIX-COLOR FLOW CYTOMETRIC ANALYSIS FOR IMMUNE PROFILE MONITORING

    Directory of Open Access Journals (Sweden)

    I. V. Kudryavtsev

    2015-01-01

    Full Text Available The article deals with applications of six-color flow analysis for studying the immune state parameters by means of flow cytometry. Whole blood from healthy donors was stained with combination of monoclonal antibodies, i.e., HLA-DR-FITC, CD16+56-PE, CD4-ECD, CD19-ECD, CD8-PC5.5, CD3-PC7, CD45-APC (Beckman Coulter, USA using a “no-wash” technology. To adjust the photomultiplier (PMT voltage, we used the tubes with each of the tested monoclonal antibodies, PMT voltage was considered optimal when the negative population was located in the middle of the first decade at the logarithmic scale. The compensatory adjustment was performed in automatic mode, using Navios software (Beckman Coulter, USA. We discuss an optimal gating strategy in order to assess the populations of interest: total T cells (CD3+CD19-, T helper cells (CD3+CD4+, cytotoxic T lymphocytes (CD3+CD8+, B cells (CD3-CD19+, NK cells (CD3-CD16+CD56+, NKT cells (CD3+CD16+CD56+, activated T lymphocytes (CD3+HLA-DR+. 

  15. Comparison of the Results of Sentive Cardiac Troponin I Detected by Two Different Detection Methods%两种不同方法检测敏感心肌肌钙蛋白Ⅰ结果比较

    Institute of Scientific and Technical Information of China (English)

    宋金萍; 孟晓辉; 达仁别克·苏利坦拜

    2012-01-01

      Objective:To comparise results of serum cardiac troponin I by Beckman-Coulter Access Immunoassay analyzer and Johnson VITROS-5600 fully automated dry chemistry analyzer,and exploring the differences as well as the linear relationship and correlation between the two. Method:45 serum samples were collected from patients presenting symptoms suggestive of acute myocardial infarction. The concentrations of serum cardiac troponin I were measured with Beckman-Coulter Access Immunoassay analyzer and Johnson VITROS-5600 fully automated dry chemistry analyzer. Data were processed by SPSS13.0 statistical analysis software.Result:Results of serum cardiac troponin I using the two methods showed no significant difference(P>0.05),while analysis shows the significant relation between the two methods(P0.05),组存在显著相关性(P<0.05).结论:本实验室采用的这两种敏感cTnI的检测结果一致性较好.

  16. Decreasing Quality of the New Generations of Anti-Müllerian Hormone Assays

    Directory of Open Access Journals (Sweden)

    Krzysztof Lukaszuk

    2014-01-01

    Full Text Available Anti-Müllerian hormone (AMH measurements are widely used to optimize the stimulation protocols. First generation AMH kits correlated well with ovarian reserve and response to stimulation. In the present study we aimed to asses if the new generation kits share the same accurate correlations. Retrospective data were collected from 8323 blood samples. For comparison we used Immunotech I generation kit (ImI 4035 samples, Beckman Coulter II generation kit RUO (BCII RUO 3449, samples and Beckman Coulter II generation kit with IVD certificate (BCII IVD 839 samples. We compared average AMH concentrations measured with different kits, as well as correlation between kits. We also compared average AMH concentrations in sera collected on different cycle days and samples of different quality of preservation. AMH serum concentrations differed for each kit, ranging 4.4 ± 4.12 (mean ± SD for the ImI, 2.68 ± 3.15 for the BCII RUO, and 1.64 ± 2.85 for BCII IVD. The mean differences from an adjusted regression model were −48.7%, −40%, and −69.2%, respectively. In conclusion, the changes of the BC AMH kits are unpredictable; however, the improvement of them is still possible. It would be very dangerous to use elaborated stimulation protocol (based on the Ist generation AMH results with the results from the IInd generation assays.

  17. Successfully accelerating translational research at an academic medical center: The University of Michigan-Coulter Translational Research Partnership Program.

    OpenAIRE

    Kenneth J Pienta

    2010-01-01

    Translational research encompasses the effective movement of new knowledge and discoveries into new approaches for prevention, diagnosis, and treatment of disease. There are many roadblocks to successful bench to bedside research, but few have received as much recent attention as the “valley of death”. The valley of death refers to the lack of funding and support for research that moves basic science discoveries into diagnostics, devices, and treatments in humans, and is ascribed to be the re...

  18. Determination of cell volume during equilibrium freezing process

    Institute of Scientific and Technical Information of China (English)

    ZHAO Gang; GAO Dayong; HE Liqun; WANG Peitao; DING Weiping; XIE Xiaojian; LIU Zhong; ZHANG Haifeng; SHU Zhiquan; LUO Dawei

    2003-01-01

    A new type electronic particle counter (EPC, MultisizerTM 3, Beckman Coulter Inc., USA) was used to determine the volumes of human red blood cells (RBCs) in NaCl solutions of different osmolalities. The thermodynamics model describing cell response during freezing process was used to simulate the volume change of RBC in 0.9% NaCl solution during equilibrium freezing process. It was assumed that the effect of temperature on cell volume can be neglected compared to that of osmolality, then by using the phase diagram for the binary system sodium chloride/water, the osmolalities of the NaCl solution under different sub-zero temperatures can be obtained (converted from mass concentration), then the calculated values of RBC volumes can be validated by the experiments.

  19. Comparison study of trapped water in human erythrocytes by EPC and DSC method and its application

    Institute of Scientific and Technical Information of China (English)

    ZHAO; Gang; HE; Liqun; GUO; Xiaojie; LIU; Zhong; LUO; Dawei

    2004-01-01

    The novel differential scanning calorimetry method for determining trapped water volume of human red blood cell during freezing process has been reexamined. Results show that the final erythrocyte volume is 53% of its isotonic volume after freezing to -40℃. An electronic particle counter (MultisizerTM III, Beckman Coulter Inc., USA) was used to measure cell volume changes in response to hypertonic solution. Using this approach, when extracellular solution was 3186 mOsm, the equilibrium cell volume was found to be 57% of its isotonic value. Both results indicate that 34%-40% of intracellular water is trapped and cannot respond to osmotic difference between intra- and extracellular solution. These findings are consistent with the published data: at least 20%-32% of the isotonic cell water volume is retained within RBCs during freezing. Some applications of the values of trapped water are addressed.

  20. Establish and compare the reference intervals of 6 serum enzyme items on the dry and wet chemistry analyzer among healthy children aged from 3 to 6 years in a region%某地区3~6周岁健康儿童干湿化学分析仪6个血清酶项目参考区间的建立与比较研究

    Institute of Scientific and Technical Information of China (English)

    曹科; 马东礼; 罗小娟; 黄宝兴; 吴跃平; 马金香

    2012-01-01

    Objective To establish and compare the reference intervals of 6 serum enzyme items on the dry and wet chemistry analyzer among healthy children aged from 3 to 6 years in Shenzhen,provide better references for clinical diagnosis and treatment. Methods The stratified randomized cluster sampling method was used to select 391 healthy children aged from 3 to 6 years, AST, LDH、ALT、ALP、GGT、CK in venous blood were detected by the VITROS 350 and Beckman Coulter LX20,and data were analyzed by statistical methods. Results The reference intervals showed no significant difference between boys and girls except CK on the dry and Wet chemistry analyzer respectively(P>-0. 05). But the results of 6 serum enzyme items showed significant difference be tween the VITROS 350 and Beckman Coulter LX20(P<0. 05). Conclusion The results showed significant difference between the VITROS 350 and Beckman Coulter LX20,the reference intervals of 6 serum enzyme items on the dry and wet chemistry analyzer a mong healthy children aged from 3 to 6 years in Shenzhen should be established according to different methods.%目的 建立和比较深圳地区3~6周岁健康儿童干湿化学分析仪6个血清酶项目的 参考区间,更好地为临床诊断和治疗服务.方法 采用随机分层抽样筛选出391名健康儿童抽取静脉血,用美国强生Vitros 350干式化学分析仪和美国Beckman Coulter LX20湿化学分析仪进行血清天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、γ-谷氨酰转肽酶(GGT)、肌酸激酶(CK)检测并分析结果.结果 该地区3~6周岁健康儿童两种方法内不同性别血清AST、LDH、ALT、ALP、GGT结果比较,差异均无统计学意义(P>0.05);CK结果差异均有统计学意义(P<0.05);6个血清酶项目两种方法间结果比较,差异均有统计学意义(P<0.05).结论 6个血清酶项目两种方法间结果比较均有显著性差异,应该针对不同方

  1. POCT法和常规检测法在BNP检测中的对比分析%Comparative analysis of POCT and routine test in BNP testing

    Institute of Scientific and Technical Information of China (English)

    朱小飞; 季明德; 李思洋; 葛亮; 顾万建

    2014-01-01

    Objective To analyze the relevance between the point-of-care testing (POCT) and routine test in BNP testing .Meth-ods The whole blood samples or plasma samples from 40 inpatients were detected brain natriuretic peptide (BNP) by the Alere Triage® MeterPro fluorescence immunoassay analyzer (POCT ) or the Beckman Coulter Access ®2 chemiluminescence analyzer (routine test) ,respectively .The acquired data were subjected to comparative analysis according to the CLCS EP 9-A2 .Results The linear regression of the BNP content in the blood samples detected by POCT and the routine test was good ,the correlation coeffi-cient(r) was 0 .999 7 .Conclusion POCT and the routine test have good correlation in BNP testing .POCT for BNP testing has higher reliability and is applicable for clinical detection .%目的:比较即时检验(POCT )法和常规检测法在B型钠脲肽(BNP)检测中的相关性。方法运用 Alere Triage®MeterPro(美国)荧光免疫分析仪或Beckman Coulter Access®2化学发光仪分别检测40份住院患者全血或血浆样本中BNP含量,按照CLCS EP9-A2文件要求进行对比分析。结果 POCT法和常规检测法检测住院患者血样BNP含量的线性回归良好,相关系数(r)=0.9997。结论 POCT法和常规检测法在BNP检测中的相关性良好,POCT 法具有较高的可靠性,可以用于临床检测。

  2. Reflotron-Hemoglobin干式血红蛋白检测系统的性能评价%Evaluation on the performance of Reflotron-Hemoglobin dry-chemistry system

    Institute of Scientific and Technical Information of China (English)

    陈骊婷; 王剑飚; 林孝怡; 石厚荣

    2014-01-01

    Objective To evaluate Reflotron-Hemoglobin dry-chemistry system for the measurement of hemoglobin. Methods The within-run and between-run precisions, linearity, correlation and reference range of Reflotron-Hemoglobin dry-chemistry system was verified.Results The within-run precision was 2.54%-2.84%,and the between-run precision was 0.68%-3.38% for Reflotron-Hemoglobin dry-chemistry system,respectively.The linearity range was 52-191 g/L .The results of Reflotron-Hemoglobin dry-chemistry system were correlated well with those of Beckman-Coulter LH750 system,and the correlation equation wan Y=1.031 6X-2.796 9.The verification coincidence rate for reference range was 100%.Conclusions The within-run and between-run precisions of Reflotron-Hemoglobin dry-chemistry system for the determination of hemoglobin are good with accurate results for both normal and abnormal samples.The reference range can be used in this system.%目的:对Reflotron-Hemoglobin干式血红蛋白检测系统进行性能评价。方法对仪器的批内精密度、批间精密度、线性、相关性及参考范围进行验证。结果 Reflotron-Hemoglobin干式血红蛋白检测系统的批内精密度为2.54%~2.84%;批间精密度为0.68%~3.38%;线性范围为52~191 g/L;与 BECKMAN-COULTER LH750全自动血液分析仪的相关方程为 Y=1.0316X-2.7969;参考区间验证符合率为100%。结论Reflotron-Hemoglobin 干式血红蛋白检测系统批内、批间精密度较好;在正常及病理范围内检测结果可靠;常用参考区间适用于本仪器。

  3. 宫颈妊娠病人双侧子宫动脉栓塞术前后血清AMH检测%SERUM ANTI-MULLERIAN HORMONE CONCENTRATION IN PATIENTS WITH CERVICAL PREGNANCY BEFORE AND AFTER BILATERAL UTERINE ARTERY EMBOLIZATION

    Institute of Scientific and Technical Information of China (English)

    左建新; 任立盛; 邵翠华; 刘美新; 王言奎

    2014-01-01

    目的 通过血清抗苗勒管激素(AMH)的测定,评价双侧子宫动脉栓塞术对卵巢储备功能的影响.方法 采用Beckman Coulter ELISA法检测20例宫颈妊娠病人双侧子宫动脉栓塞术前及术后1、6个月血清AMH水平,并比较其变化.结果 宫颈妊娠病人子宫动脉栓塞术前至术后1、6个月血清AMH水平分别为(7.99±4.15)、(7.11±3.02)和(8.33±4.87)mg/L,手术前后比较差异无显著性(F=0.93,P>0.05).结论 子宫动脉栓塞术治疗宫颈妊娠不影响卵巢储备功能.

  4. Apoptosis induced by (di-isopropyloxyphoryl-Trp)2-Lys-OCH3 in K562 and HeLa cells

    Indian Academy of Sciences (India)

    Feng Liu; Shi-Ying Liu; Ping Xu; Zhen-Hua Xie; Guo-Ping Cai; Yu-Yang Jiang

    2008-03-01

    According to the method used in our laboratory, our group synthesized (DIPP-Trp)2-Lys-OCH3. It inhibited the proliferation of K562 and HeLa cells in a dose- and time-dependent manner with an IC50 of 15.12 and 42.23 M, respectively. (DIPP-Trp)2-Lys-OCH3 induced a dose-dependent increase of the G2/M cell population in K562 cells, and S cell population in HeLa cells; the sub-G0 population increased dramatically in both cell lines as seen by PI staining experiments using a FACS Calibur Flow cytometer (BeckmanCoulter, USA). Phosphatidylserine could significantly translocate to the surface of the membrane in (DIPP-Trp)2-Lys-OCH3-treated K562 and HeLa cells. The increase of an early apoptotic population was observed in a dose-dependent manner by both annexin-FITC and PI staining. It was concluded that (DIPP-Trp)2-Lys-OCH3 not only induced cells to enter into apoptosis, but also affected the progress of the cell cycle. It may have arrested the K562 and HeLa cells in the G2/M, S phases, respectively. The apoptotic pathway was pulsed at this point, resulting in the treated cells entering into programmed cell death. (DIPP-Trp)2-Lys-OCH3 is a potential anticancer drug that intervenes in the signalling pathway.

  5. An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems

    Directory of Open Access Journals (Sweden)

    Verschraegen Gerda

    2005-03-01

    Full Text Available Abstract Background Currently, most laboratories identify yeasts routinely on the basis of morphology and biochemical reactivity. This approach has quite often limited discriminatory power and may require long incubation periods. Due to the increase of fungal infections and due to specific antifungal resistence patterns for different species, accurate and rapid identification has become more important. Several molecular techniques have been described for fast and reliable identification of yeast isolates, but interlaboratory exchangeability of identification schemes of molecular techniques has hardly been studied. Here, we compared amplified ITS2 fragment length determination by an ABI Prism 310 (Applied Biosystems, Foster City, Ca. capillary electrophoresis system with that obtained by a CEQ8000 (Beckman Coulter, Fullerton, Ca. capillary electrophoresis system. Results Although ITS2 size estimations on both systems differed and separate libraries had to be constructed for each system, both approaches had the same discriminatory power with regard to the 44 reference strains, identical identifications were obtained for 39/ 40 clinical isolates in both laboratories and strains from 51 samples were correctly identified using CEQ8000, when compared to phenotypic identification. Conclusion Identification of yeasts with ITS2-PCR followed by fragment analysis can be carried out on different capillary electrophoresis systems with comparable discriminatory power.

  6. Ultrasonic sonoelastography of scrotum in the diagnosis of male fertility

    Directory of Open Access Journals (Sweden)

    O. B. Zhukov

    2014-12-01

    Full Text Available Objective: to determine the possibility of using detect the scrotum in assessing reproductive function of men.Materials and methods. The study was conducted in men aged 20–44 years. The first group of men was 25 patients with secretory infertility. The second group included 25 male infertility varicocele. The control group presents 12 men in the age range 22–31 year with the implementation of the reproductive function of 1 or more children. The study was performed by ultrasonic scanner expert class Acuson S2000 manufacture of the company Siemens, was used microwave sensor 18L6 HD. Sex hormones were studied on immunochemiluminescence analyzer Access 2 production Beckman Coulter (USA. Obtaining and study of ejaculate was carried out according to Recommendations of the WHO (5th edition.Results. The application of research shear transverse wave Vs men with secretory infertility and varicocele is accompanied by the increase of its value to 1.5–1.6 m/s and is characterized by 35–45 % from similar parameters of reproductive healthy men. In half of the cases studies such an increase in performance and decrease in the area of mapping the so-called “soft” areas of the epididymis is accompanied by a decrease in the concentration and actively motile sperm. The use of such method of instrumental diagnostics will allow at the stage of preliminary ultrasound screening to suspect a violation of the reproductive capacity of men.

  7. Normal lymphocyte immunophenotype in an elderly population

    Directory of Open Access Journals (Sweden)

    Sâmia Macedo Queiroz Mota Castellão Tavares

    2014-06-01

    Full Text Available OBJECTIVE: The aim of this work was to evaluate the lymphocyte immunophenotype in an elderly population.METHODS: This study enrolled 35 over 60-year-old volunteers and a control group composed of 35 young adults. The study included elderly without diseases that might affect the functioning of the immune system. These individuals were consulted by doctors and after a physical examination, laboratory tests were performed using a Beckman Coulter (r flow cytometer. The GraphPad Prism computer program was employed for statistical analysis with the level of significance being set for p-values <0.05.RESULTS: There is a statistically significant reduction in the number of lymphocytes (CD8 +, CD2 + and CD3 + cells in the elderly compared to young adults. These low rates are explained by changes attributed to aging and may be partly responsible for the reduction in the cellular immune response, lower proliferative activity and the low cytotoxicity of lymphocytes.CONCLUSION: These parameters showed greater impairment of adaptive immunity in the elderly population and can therefore explain the greater fragility of the aged body to developing diseases.

  8. [Hematological indices of rats having complete and vitamin-deficient diets enriched with dietary fibers].

    Science.gov (United States)

    Mustafina, O K; Trushina, É N; Kosheleva, O V; Pereverzeva, O G; Kodentsova, V M; Vrzhesinskaia, O A

    2013-01-01

    The hematological indices in 48 rats Wistar male with initial body weight 58.1+/- 0.5g has been studied. The rats were divided into 6 group and fed the complete semi-synthetic diet, containing 100% or 20% of vitamin mixture (Vit) with or without addition of dietary fiber (DF) in the form of wheat bran (5% of diet mass) during 4 weeks. The animals of the 1 group received 100% of vitamin mixture (100% Vit); of the 2 group--100% Vit+DF; 3 group--20% of vitamin mixture (20% Vit); 4 group--20% of vitamin mixture and DF (20% Vit+DF). The next 5 days rats from vitamin-deficient groups were fed with diets supplemented with 80% of Vit: (5 group--20% Vit+ 80% Vit; 6 group--20% Vit+DF+80% Vit). The animals fed vitamin-deficient diet lag significantly in growth from animals fed a complete diet. The growth curve of rats fed a diet with DF took an intermediate position. The studies were carried out at the Hematology analyzer "Coulter AC TTM 5 diff OV" (Beckman Coulter, USA) under the program, developed for the study of rat blood. Mean corpuscular volume (MCV) and mean corpuscular hemoglobin content (MCHC) were significantly decreased in rats with combined vitamin deficiency (20% Vit) in the diet compared with those of control group rats, while the compensatory increase in the number of red blood cells (RBC) and leukocytopenia took place. The enrichment of vitamin-deficient diet with DF (20% Vit+DF) prevented MCV and RBC changes, but MCHC left reduced in comparison with the indicator of the rats in control group. Indicators characterizing the state of platelets had no statistically significant differences between the groups. Compensation of vitamin deficiency in the diet of rats from group 5 (20% Vit+80% Vit) led only to the normalization of MCV. After vitamin restore in the diet of group 6 (20% Vit+DF+80% Vit) all investigated parameters were indistinguishable from the parameters of the control group. This indicates a positive effect of wheat bran DF consumption on the studied

  9. 复凝聚法制备桃金娘油肠溶微囊%Enteric myrtle oil microcapsules by complex coacervation

    Institute of Scientific and Technical Information of China (English)

    蔡翠芳; 曾雪萍; 何海冰; 齐娜; 唐星

    2011-01-01

    Objective To prepare myrtle oil enteric-coated microcapsules using a complex coacervation method and evaluate the in vitro performance. Methods Sodium alginate,calcium chloride and chitosan were selected as the materials for myrtle oil loaded microcapsules; surface morphology and size of microcapsules were characterized by scanning electronic microscopy (SEM) and laser diffraction particle size analyzer (Beckman Coulter LS 230). The headspace gas chromatography (HS-GC) method was employed to determine the loading capacity and encapsulation efficiency. Results Using orthogonal design, formulation and process were optimized as follows :sodium alginate concentration was 25 g·L-1, chitosan concentration was 3 g ·L-1,condensation rate was 5 mL·min-1 and time was 60 min. The drug loaded enteric microcapsules showed shrunken surface,narrow size distribution with average particle size of( 14. 23 ± 1.45) μm,loading capacity of(11. 3 ±0.4)% , and good acid resistance. Conclusions Optimized myrtle oil loaded microcapsules demonstrates higher dose, enteric features and good reproducibility. This method can be used for the preparation of myrtle oil enteric microcapsules.%目的 采用复凝聚法制备桃金娘油肠溶微囊,并对其体外性质进行评价.方法 选用海藻酸钠、氯化钙、壳聚糖为囊材采用复凝聚法制备桃金娘油微囊,用扫描电子显微镜(scanning electron microscope,SEM),Beckman Coulter LS 230激光粒度仪表征了微囊表面形态及粒径,采用顶空进样-GC色谱法测定了载药量和包封率.结果 正交设计优化处方和工艺如下:海藻酸钠质量浓度为25g·L-1、壳聚糖质量浓度为3 g·L-1、凝聚速度为5 mL·min-1和凝聚时间为60min,所得微囊粒径为(14.23±1.45)μm,载药质量分数为(11.3±0.4)%,包封率为(73.6±2.5)%.微囊具有 耐酸和肠溶性能,表面褶皱,粒径分布均匀.结论 复凝聚法可用于桃金娘油肠溶微囊的制备.

  10. Study on Profiling Mitochondria Proteome of Human Fetal Liver by Multi-dimensional Liquid Chromatography and Liquid Chromatography-Ion Trap Mass Spectrometry%多维液相色谱及液相色谱-离子阱质谱法研究人胎肝线粒体蛋白质组

    Institute of Scientific and Technical Information of China (English)

    张养军; 石蓉; 孟庆芳; 王京兰; 蔡耘; 朱云平; 贺福初; 钱小红

    2004-01-01

    In proteomics study, because there were biases of two-dimensional gel electrophoresis in molecular weights, pH and hydrophobicity, multi-dimensional protein identification technology (MuD-PIT)was proposed. Due to the core part of the technique being digestion of total proteins in the first step, some drawbacks appeared, such as complicating the separation of samples, dominating the influence of proteins with high abundance on the identification of proteins with low abundance and losing some information of proteins,for example, post-translational modification et al. , these resulted in the limitation of applications for the method. Taking into account of those factors mentioned above, when studying the mitochondria proteome of human fetal liver with great significance in life process, Proteome Lab PF2D system (Beckman Coulter, USA) was employed. The fractions of extract from fetal liver tissues by saccharose- density gradient centrifugation were prepared on a high velocity centrifuge. The desalting and solvent exchanging of the fractions with Start buffer (pH8. 5, Beckman Coulter, USA) were carried out on a PD-10(Sephadex G-25, 5 000Da) desalting column (amersham pharmacia biotech, Wikstroms,Sweden) according to the procedures described in its product instruction. Then the treated fractions were separated with chromatofocusing and reversed-phase liquid chromatography in tandem, respectively. After concentration and in-solution digestion of protein fractions,the peptide mixture was lyophilized, then resolved with 5% aqueous acytonitrile plus 0.1% formic acid, and finally analyzed with microLC-ESI-ion trap mass spectrometry(Thermo Finnigan, San Jose, CA, USA) and CapLC-ESI-QTOF mass spectrometry (Waters, USA). Mass spectrometric data was searched against IPI protein database with Thermo SEQUEST (Thermo Finnigan, San Jose, CA, USA) and Mascot. A primary database of mitochondrial proteome of human fetal liver was constructed, which could play central role in the large

  11. 中性粒细胞和淋巴细胞VCS参数在巨幼细胞贫血中的研究%Study on VCS Data of Neutrophil and Lymphocyte in Megaloblastic Anemia

    Institute of Scientific and Technical Information of China (English)

    张丽霞; 高丽; 陆琳; 张杰; 刘健; 潘世扬

    2013-01-01

    Objective To assess changes in neutrophil and lymphocyte volume,conductivity and laser light scatter (VCS) parameter in megaloblastic anemia. Methods Neutrophil VCS parameters: MNV (volume) ,MNC (conductivity), MNS (light scattering) and lympho cyte VCS parameters: MLV, MLC, MLS were studied in a group of 120 healthy subjects and two groups of patients with megaloblastic anemia:19 cases of folate deficiency,30 cases of VitB12 deficiency,by using a LH-750 hematology analyzer (Beckman Coulter). Results All the neutrophil and lymphocyte VCS parameters were higher in comparison to healthy controls. The MNV,MLV and MLS value were significantly increased (t=-19. 29~-7. 62,P0. 05). Conclusion The neutrophil and lymphocyte VCS parameters may be useful for diagnosis in megaloblastic anemia.%目的 探讨外周血中性粒细胞和淋巴细胞VCS参数在巨幼细胞贫血(megaloblastic anemia,MA)诊断中的应用价值.方法 选取2011年1月~12月南京医科大学第一附属医院门诊已经通过叶酸、VitB12检查和骨髓涂片确诊的MA患者49例,并分为MA1组(叶酸缺乏组,19例)和MA2组(VitB12缺乏组,30例),以120例健康体检者(男性60例,女性60例)作为对照组,其血常规分析结果正常,并排除血液系统疾病及其他器质性疾病.通过LH750血液分析仪(Beckman Coulter)进行血常规检测,分析中性粒细胞和淋巴细胞体积(volume,V)、电导(conductivity,C)和光散射(light scattering,S)参数,用Stata/SE9.2统计软件进行统计分析.结果 MA1组和MA2组患者中性粒细胞平均体积(mean neutrophil volume,MNV)、平均电导率(mean neutrophil conductivity,MNC)和平均散射率(mean neutrophil scatter,MNS)以及淋巴细胞平均体积(mean lymphocyte volume,MLV)、平均电导率(mean lymphocyte conductivity,MLC)和平均散射率(mean lymphocyte scatter,MLS)均高于对照组,其中MA1组和MA2组的MNV,MLV和MLS和健康对照组差异均具统计学意义(t=-19.29~-7.62,P值均<0.01).MA患者中

  12. 多维液相色谱及液相色谱-离子阱质谱法研究人胎肝线粒体蛋白质组

    Institute of Scientific and Technical Information of China (English)

    张养军; 石蓉; 孟庆芳; 王京兰; 蔡耘; 朱云平; 贺福初; 钱小红

    2004-01-01

    In proteomics study, because there were biases of two-dimensional gel electrophoresis in molecular weights, pH and hydrophobicity, multi-dimensional protein identification technology (MuD-PIT)was proposed. Due to the core part of the technique being digestion of total proteins in the first step, some drawbacks appeared, such as complicating the separation of samples, dominating the influence of proteins with high abundance on the identification of proteins with low abundance and losing some information of proteins,for example, post-translational modification et al. , these resulted in the limitation of applications for the method. Taking into account of those factors mentioned above, when studying the mitochondria proteome of human fetal liver with great significance in life process, Proteome Lab PF2D system (Beckman Coulter, USA) was employed. The fractions of extract from fetal liver tissues by saccharose- density gradient centrifugation were prepared on a high velocity centrifuge. The desalting and solvent exchanging of the fractions with Start buffer (pH8. 5, Beckman Coulter, USA) were carried out on a PD-10(Sephadex G-25, 5 000Da) desalting column (amersham pharmacia biotech, Wikstroms,Sweden) according to the procedures described in its product instruction. Then the treated fractions were separated with chromatofocusing and reversed-phase liquid chromatography in tandem, respectively. After concentration and in-solution digestion of protein fractions,the peptide mixture was lyophilized, then resolved with 5% aqueous acytonitrile plus 0.1% formic acid, and finally analyzed with microLC-ESI-ion trap mass spectrometry(Thermo Finnigan, San Jose, CA, USA) and CapLC-ESI-QTOF mass spectrometry (Waters, USA). Mass spectrometric data was searched against IPI protein database with Thermo SEQUEST (Thermo Finnigan, San Jose, CA, USA) and Mascot. A primary database of mitochondrial proteome of human fetal liver was constructed, which could play central role in the large

  13. 同型半胱氨酸和叶酸、维生素B12联合检测在心血管疾病中的作用%Combinative Function of Hcy,FA and VitB12 in Analysis of Cardiovascular Disease

    Institute of Scientific and Technical Information of China (English)

    孟岩; 王蕾; 崔桂华; 郝彦平; 刘辉; 靳松

    2011-01-01

    Objective To study the relationship between cardiovascular disease and serum,homocysteine (Hcy),folic acid (FA) ,and the density of VitB12. Methods To determine the relativity between Hcy and the level of FA,VitB12.120 cases were selected after coronary arteriography. Enzymatic cycling assay was introduced to test the level of serum Hcy by using an apparatus,Hitachi 7170,automatic biochemical analyzer. Automated microparticle chemiluminescence immunoassay ahalyzer (Beckman-Coulter ACCESS) was used to test the level of FA,VitB12. Results ① The level of Hcy in group with coronary disease was higher than those in control group(P<0. 0l). ② Patients with coronary artery disease Hcy level and serum FA and VitB12 was a negative correlation(r =- 0. 64;r =- 0. 44,P<0.01). ③Patients with coronary artery disease by taking FA,VitB12 and VitB6 after treatment serum Hcy concentration obvious(r =- 0. 64;r =- 0. 44,P<0. 0l). Conclusion The high level of Hcy is a higher risk of independent factor with coronary disease.%目的 探讨血清同型半胱氨酸(Hcy)、叶酸(FA)、维生素B12(Vit B12)浓度与心血管病的关系.方法 选择经冠状动脉造影检查的患者共120例,血清同型半胱氨酸(Hcy)采用日立7170全自动生化分析仪,方法为循环酶法;叶酸(FA)、维生素B12(Vit B12)采用Beckman-Coulter ACCESS全自动微粒子化学发光免疫分析仪检测.观察Hcy与FA,VitB12水平变化的相关性.结果 ①冠心病患者血清Hcy含量显著高于对照组(P<0.01).②冠心病患者血清Hcy水平与FA,VitB12呈负相关(r=-0.64;r=-0.44,P<0.01).③冠心病患者经服用叶酸(FA)、维生素B12(VitB12)、维生素B6(VB6)治疗后,血清Hcy浓度降低明显(P<0.01).结论 高同型半胱氨酸水平是冠心病的独立危险因素.

  14. Iron status and its determinants in a nationally representative sample of pregnant women.

    Science.gov (United States)

    Vandevijvere, Stefanie; Amsalkhir, Sihame; Van Oyen, Herman; Egli, Ines; Ines, Egli; Moreno-Reyes, Rodrigo

    2013-05-01

    Iron-deficiency anemia is associated with adverse neonatal health outcomes. Iron status and its determinants were assessed in a representative sample of Belgian pregnant women. Blood samples were collected and a questionnaire was completed face-to-face. Hemoglobin (Hb) and mean cell volume were measured using a Beckman Coulter Hematology Analyzer and serum ferritin (SF) and transferrin receptor (sTfr) concentrations by immunoassay. In total, 55 obstetric clinics and 1,311 pregnant women were included. Approximately 40% of third-trimester and 6% of first-trimester women had SF levels less than 15 μg/L. Approximately 21% of third-trimester and 4% of first-trimester women had anemia (Hb iron-deficient nonanemic (SF iron-deficiency anemia (SF iron deficiency (sTfr >8.5 mg/L). The median body iron stores were 8.1 mg/kg among first-trimester women, but only 3.6 mg/kg among third-trimester women. SF levels were significantly positively associated with age and education level, and were higher among nulliparous women and lower among North-African women. sTfr concentrations were significantly negatively associated with age and were lower among smokers, nulliparous women, and women who planned their pregnancy. Despite the fact that two thirds of Belgian pregnant women took iron-containing supplements, iron deficiency and iron-deficiency anemia were frequent in third-trimester women. The World Health Organization regards this as a moderate public health problem. National iron supplementation guidelines are needed in Belgium to optimize iron status during pregnancy.

  15. Diurnal Variations of Circulating Extracellular Vesicles Measured by Nano Flow Cytometry.

    Directory of Open Access Journals (Sweden)

    Kirsty M Danielson

    Full Text Available The identification of extracellular vesicles (EVs as intercellular conveyors of biological information has recently emerged as a novel paradigm in signaling, leading to the exploitation of EVs and their contents as biomarkers of various diseases. However, whether there are diurnal variations in the size, number, and tissue of origin of blood EVs is currently not known, and could have significant implications when using EVs as biomarkers for disease progression. Currently available technologies for the measurement of EV size and number are either time consuming, require specialized equipment, or lack sufficient accuracy across a range of EV sizes. Flow cytometry represents an attractive alternative to these methods; however, traditional flow cytometers are only capable of measuring particles down to 500 nm, which is significantly larger than the average and median sizes of plasma EVs. Utilizing a Beckman Coulter MoFlo XDP flow cytometer with NanoView module, we employed nanoscale flow cytometry (termed nanoFCM to examine the relative number and scatter distribution of plasma EVs at three different time points during the day in 6 healthy adults. Analysis of liposomes and plasma EVs proved that nanoFCM is capable of detecting biologically-relevant vesicles down to 100 nm in size. With this high resolution configuration, we observed variations in the relative size (FSC/SSC distributions and concentration (proportions of EVs in healthy adult plasma across the course of a day, suggesting that there are diurnal variations in the number and size distribution of circulating EV populations. The use of nanoFCM provides a valuable tool for the study of EVs in both health and disease; however, additional refinement of nanoscale flow cytometric methods is needed for use of these instruments for quantitative particle counting and sizing. Furthermore, larger scale studies are necessary to more clearly define the diurnal variations in circulating EVs, and thus

  16. Detection of an IncA/C plasmid encoding VIM-4 and CMY-4 β-lactamases in Klebsiella oxytoca and Citrobacter koseri from an inpatient in a cardiac rehabilitation unit.

    Science.gov (United States)

    Caltagirone, Mariasofia; Bitar, Ibrahim; Piazza, Aurora; Spalla, Melissa; Nucleo, Elisabetta; Navarra, Antonella; Migliavacca, Roberta

    2015-07-01

    A 62-year-old patient was transferred to the cardiac rehabilitation unit of the I.R.C.C.S. Fondazione S. Maugeri after undergoing a heart transplantation at the Acute Care Hospital I.R.C.C.S. S. Matteo of Pavia. On 1 August 2013 and during hospitalization in the rehabilitation unit, Klebsiella oxytoca and Citrobacter koseri clinical isolates were simultaneously recovered from the patient's preputial swab. Both the K. oxytoca and C. koseri strains were carbapenem- resistant by MicroScan System (Beckman Coulter). Carbapenem-resistant K. pneumoniae had previously been reported in the same rehabilitation facility. The aim of the study was to identify the carbapenem resistance mechanisms among the enterobacterial species recovered. Phenotypic screening tests useful to detect the β-lactamases/carbapenemases were performed. Carbapenem MICs were obtained by Etest. AmpC and MBL encoding genes were identified by PCR and sequencing. Conjugation assays and plasmid characterization were performed. Both of the K. oxytoca and C. koseri isolates were multi drug resistant, showing resistance to amoxicillin-clavulanic acid, three generation cephalosporins, ertapenem (K. oxytoca MIC, >32 mg/L; C. koseri MIC, 4 mg/L), imipenem (K. oxytoca MIC, 4 mg/L; C. koseri MIC, 12 mg/L), thrimethoprim sulphamethoxazole and gentamicin. Susceptibility was retained to fluoroquinolones, colistin and tigecycline. Molecular characterization confirmed the co-presence of blaCMY-4 and blaVIM-4 determinants in a 150 Kb transferable plasmid of IncA/C group. This case is the first detection in Italy of the K. oxytoca and C. koseri clinical isolates co-producing the CMY-4 and VIM-4 enzymes.

  17. The Effects of Short-Term Intensive Exercise on Levels of Liver Enzymes and Serum Lipids in Kick Boxing Athletes

    Directory of Open Access Journals (Sweden)

    Ömer Kaynar

    2016-03-01

    Full Text Available Objective: In this study, it was aimed to evaluate the ef­fects of short-term intensive exercise on liver enzymes and serum lipid levels with kick boxing athletes. Methods: 23 voluntary athletes who were between the ages of 15-46 and who engaged in kick–boxing have tak­en place this study. Athletes were made to do 45 minutes of warming-up, breathing, and stretching and 50 minutes of technical and tactical practices and then they were made to do a training match, which is equal to a 2 min­utes 3 circuits (1 minute rest kick-box match. In venous blood samples which were taken from athletes before and after training, aspartate aminotransferase (AST, alanine aminotransferase (ALT, alkaline phosphatase (ALP and gamma glutamine transpeptidase (GGT, enzyme activity and total cholesterol, high-density lipoprotein-cholesterol (HDL-C, low density lipoprotein-cholesterol (LDL-C and triglycerides serum levels were analyzed via spectropho­tometric method in Beckman Coulter AU 5800 auto ana­lyzer. Body composition measurements of athletes were made with Tanita TBF 300 brand device, which works with bio-impedance analysis (BIA system. Results: As a result of our study, statistically increases in serum ALT, AST, ALP and GGT enzyme activities and in serum total cholesterol, HDL-C and LDL-C levels were detected following short-term intensive exercise, but no significant difference was observed in TG levels after in­tensive exercise. Conclusion: The blows to the abdomen during kickbox­ing sports competitions result in increased liver enzymes and increased serum lipids may occur to meet energy de­mand of the body during exercise.

  18. Proteomic methodological recommendations for studies involving human plasma, platelets, and peripheral blood mononuclear cells.

    Science.gov (United States)

    de Roos, Baukje; Duthie, Susan J; Polley, Abigael C J; Mulholland, Francis; Bouwman, Freek G; Heim, Carolin; Rucklidge, Garry J; Johnson, Ian T; Mariman, Edwin C; Daniel, Hannelore; Elliott, Ruan M

    2008-06-01

    This study was designed to develop, optimize and validate protocols for blood processing prior to proteomic analysis of plasma, platelets and peripheral blood mononuclear cells (PBMC) and to determine analytical variation of a single sample of depleted plasma, platelet and PBMC proteins within and between four laboratories each using their own standard operating protocols for 2D gel electrophoresis. Plasma depleted either using the Beckman Coulter IgY-12 proteome partitioning kit or the Amersham albumin and IgG depletion columns gave good quality gels, but reproducibility appeared better with the single-use immuno-affinity column. The use of the Millipore Filter Device for protein concentration gave a 16% ( p appears as a single abundant spot. The average within-laboratory coefficient of variation (CV) for each of the matched spots after automatic matching using either PDQuest or ProteomWeaver software ranged between 18 and 69% for depleted plasma proteins, between 21 and 55% for platelet proteins, and between 22 and 38% for PBMC proteins. Subsequent manual matching improved the CV with on average between 1 and 16%. The average between laboratory CV for each of the matched spots after automatic matching ranged between 4 and 54% for depleted plasma proteins, between 5 and 60% for platelet proteins, and between 18 and 70% for PBMC proteins. This variation must be considered when designing sufficiently powered studies that use proteomics tools for biomarker discovery. The use of tricine in the running buffer for the second dimension appears to enhance the resolution of proteins especially in the high molecular weight range.

  19. A technique for dating toxoplasmosis in pregnancy and comparison with the Vidas anti-toxoplasma IgG avidity test.

    Science.gov (United States)

    Flori, P; Bellete, B; Crampe, C; Maudry, A; Patural, H; Chauleur, C; Hafid, J; Raberin, H; Tran Manh Sung, R

    2008-03-01

    A comparative evaluation of 384 selected sera was performed using the Beckman Coulter Access and Abbott Axsym Toxo-IgG assays. The Axsym assay yields positive early results following infection, while the Access assay gives higher titres during chronic infection. The ratio between the two complementary tests, Axsym Toxo-IgG/Access Toxo-IgG (Ax/Ac), was compared with the Vidas anti-Toxoplasma IgG avidity index (AI). The Ax/Ac ratio decreased progressively as the time between infection and sampling increased. The mean Ax/Ac values (+/-SE) were 2.50 (+/-0.26), 2.14 (+/-0.13), 2.33 (+/-0.22), 1.34 (+/-0.09), 1.32 (+/-0.10), 0.92 (+/-0.08) and 0.74 (+/-0.07) for groups of sera sampled at 1, 2, 3, 4-5, 6-8, 9-12 and 13-24 months, respectively, after infection in pregnant women. These values were much smaller for cases with chronic infection (>24 months), i.e., 0.56 (+/-0.03), 0.44 (+/-0.04) and 0.53 (+/-0.04), respectively, for pregnant women and immunodepressed patients with and without reactivation. Taking a ratio of 1 as a threshold for recent infection, the patients in the groups sampled at 1, 2 and 3 months had Ax/Ac ratios >1 in 49/50 (98%), 53/55 (96.4%) and 36/36 (100%) cases, respectively. Thus, an Ax/Ac ratio of <1 in serum from a pregnant woman allows a recent infection (<3 months) to be excluded. This technique has the advantage of yielding positive results that develop much more rapidly than the AI, thereby helping to reassure large numbers of pregnant women and avoiding costly and unnecessary prophylactic treatment and follow-up.

  20. Comparable flow cytometry data can be obtained with two types of instruments, Canto II, and Navios. A GEIL study.

    Science.gov (United States)

    Solly, Françoise; Rigollet, Lauren; Baseggio, Lucille; Guy, Julien; Borgeot, Jessica; Guérin, Estelle; Debliquis, Agathe; Drenou, Bernard; Campos, Lydia; Lacombe, Francis; Béné, Marie C

    2013-12-01

    Flow cytometry (FC) instruments settings classically rely on local establishment of photomultipliers (PMT) voltages adapted to the measurements expected to be performed. In the era of multiparameter FC (MFC), it appears more and more desirable that comparable patterns of fluorescence are obtained in different settings. This relies on a harmonization of settings between instruments. Although this has been shown to be feasible within a given brand of flow cytometers, little information is available about broader comparisons in a given center or in a multicenter fashion. Here, we report a two-phase series of experiments first performed between a Canto II (BD Biosciences) and a Navios (Beckman Coulter) instruments in the same center. PMT values adjusted on the reference instrument (RI) Canto II were used to establish target values for PMT settings on the paired Navios practice instrument (PI). This allowed to show the good correlation of all but peaks 1 and 2 of Rainbow(®) beads between RI and PI. Using 4- or 8-color stained leukocytes, the similitude of the settings was further confirmed. A complex set of matrices was then established between five centers all equipped with both instruments. Using Bland & Altman difference comparisons for median fluorescence values, it was shown that using either Rainbow beads or CD16 stained polymorphonuclears to set-up target values on the RI CantoII, highly superimposable results could be obtained on all 9 PI. The latter were obtained using Rainbow beads or Compbeads(®) for comparisons. In summary, this two-phase study demonstrates the feasibility of different methods allowing for a robust harmonization of settings for MFC. PMID:24151234

  1. THE VARIATION OF PLASMATIC CONCENTRATION OF HAEMOGLOBIN AND THE EVALUATION OF HEAT SHOCK PROTEINS IN RHEUMATIC PATIENTS

    Directory of Open Access Journals (Sweden)

    Claudia Vlad

    2012-06-01

    Full Text Available Rheumatoid arthritis is a systemic inflammatory disease with still unknown aetiology. The purpose of our study was to comparatively investigate the level of haemoglobin, as well as of the heat shock proteins HSP60 and HSP70 as well as their specific antibodies serum levels in rheumatic patients, in order to evaluate their potential role as an aid in the diagnosis of this chronic pathology. This study was performed on patients with rheumatoid polyarthritis. The haemoglobin plasmatic concentration was assessed by a quantitative determination using the automatic analyzer Beckman Coulter Act5diff, while the thermal shock proteins HSP 60, HSP 70 as well as their respective serum antibodies were determined by Western blot method and ELISA assay, respectively. All the patients involved in this study exhibited low values of haemoglobin, known to be an important marker of haematological disorders. These results maintain the idea that anaemia, heart disease, osteoporosis are the most frequent complication for rheumatoid arthritis. The concept of overexpression of endogenous HSPs is central to hypotheses in which HSPs are implicated in the pathogenesis of autoimmune rheumatic disease. The quantification of HSPs levels in the serum of the rheumatic patients showed that both Hsp60 and Hsp70 levels are higher, especially in those patients who had as a secondary disease, like cardiac insufficiency and obesity. The HSP60 and HSP70 antibodies were also highly expressed in our patients. These lesions could be partially due to the fact that despite the ubiquitous and high homology of heat shock proteins among different species, they also represent important antigenic targets of the cellular and humoral immune response. Besides the low level of haemoglobin, the presence of a high level of heat shock proteins and of their corresponding antibodies may be considered as useful markers which could be correlated with the evolution and the severity of a long chain of

  2. Mean corpuscular volume of control red blood cells determines the interpretation of eosin-5'-maleimide (EMA) test result in infants aged less than 6 months.

    Science.gov (United States)

    Ciepiela, Olga; Adamowicz-Salach, Anna; Bystrzycka, Weronika; Łukasik, Jan; Kotuła, Iwona

    2015-08-01

    Eosin-5'-maleimide (EMA) binding test is a flow cytometric test used to detect hereditary spherocytosis (HS). To perform the test sample from patients, 5-6 reference samples of red blood are needed. Our aim was to investigate how the mean corpuscular volume (MCV) of red blood cells influences on the value of fluorescence of bounded EMA dye and how the choice of reference samples affects the test result. EMA test was performed in peripheral blood from 404 individuals, including 31 children suffering from HS. Mean fluorescence channel of EMA-RBCs was measured with Cytomics FC500 flow cytometer. Mean corpuscular volume of RBCs was assessed with LH750 Beckman Coulter. Statistical analysis was performed using Graph Pad Prism. The correlation Spearman coefficient between mean channel of fluorescence of EMA-RBCs and MCV was r = 0.39, p < 0.0001. Interpretation of EMA test depends on MCV of the reference samples. If reference blood samples have lower MCV than the patients MCV, EMA test result might be negative. Due to different MCV values of RBCs in infancy and ca. Three months later, EMA test in neonates might be interpreted falsely negative. Samples from children younger than 3 months old had EMA test result 86.1 ± 11.7 %, whereas same samples that analyzed 4.1 ± 2.1 later had results of 75.4 ± 4.5 %, p < 0.05. Mean fluorescence of EMA-bound RBC depends on RBC's volume. MCV of reference samples affects EMA test results; thus, we recommend selection of reference samples with MCV in range of ±2 fL compared to MCV of patient RBC's.

  3. The routine leukocyte differential flow cytometry HematoFlow™ method: A new flagging system for automatic validation.

    Science.gov (United States)

    Allou, Kaoutar; Vial, Jean-Philippe; Béné, Marie C; Lacombe, Francis

    2015-01-01

    The complete blood cell count and white blood cell differential are the first step in the biological diagnosis of hematological diseases. Both are currently performed by automated instruments which control data and produce alerts. If such flags are activated, the automated differential cannot be validated and the operator must activate a visual blood smear review. Microscopic examination is still today the reference method despite its lack of sensitivity and reproducibility. The HematoFlow™ (Beckman Coulter) system is the first flow cytometry commercialized method designed for the routine differential. Using six markers in five colors and an automated gating strategy, it provides differentials proven to be reliable for 17 leukocyte subpopulations detection. Relying first on a retrospective analysis of 6,462 blood samples processed by HematoFlow™, thresholds were determined to detect the presence of immature granulocytes and/or blast cells. All possible gating strategy misclassifications of leukocyte subpopulations were then summarized in a systematic nomenclature leading to the development of an original flag system based on the detection of aberrant localization of cell events in specific new bivariate histograms. Ultimately, more than 50% of the results could be automatically validated using the HematoFlow™ system, without any false negative, thereby dramatically contributing to an important decrease of technicians' workload. Moreover a noticeable help was given for smear review interpretation and new immunological flags led to the confirmation of blood disease after classical immunophenotyping. These results were confirmed in a second prospective study including 15,335 cases, where more than 50% of the results were automatically validated by this new flag system. MFC stands as being more and more essential for analyzing differentials in routine and this new flag system could greatly improve its implementation. PMID:25906976

  4. Time course characterization of serum cardiac troponins, heart fatty acid-binding protein, and morphologic findings with isoproterenol-induced myocardial injury in the rat.

    Science.gov (United States)

    Clements, Peter; Brady, Sally; York, Malcolm; Berridge, Brian; Mikaelian, Igor; Nicklaus, Rosemary; Gandhi, Mitul; Roman, Ian; Stamp, Clare; Davies, Dai; McGill, Paul; Williams, Thomas; Pettit, Syril; Walker, Dana; Turton, John

    2010-08-01

    We investigated the kinetics of circulating biomarker elevation, specifically correlated with morphology in acute myocardial injury. Male Hanover Wistar rats underwent biomarker and morphologic cardiac evaluation at 0.5 to seventy-two hours after a single subcutaneous isoproterenol administration (100 or 4000 microg/kg). Dose-dependent elevations of serum cardiac troponins I and T (cTnI, cTnT), and heart fatty acid-binding protein (H-FABP) occurred from 0.5 hour, peaked at two to three hours, and declined to baseline by twelve hours (H-FABP) or forty-eight to seventy-two hours (Serum cTns). They were more sensitive in detecting cardiomyocyte damage than other serum biomarkers. The Access 2 platform, an automated chemiluminescence analyzer (Beckman Coulter), showed the greatest cTnI fold-changes and low range sensitivity. Myocardial injury was detected morphologically from 0.5 hour, correlating well with loss of cTnI immunoreactivity and serum biomarker elevation at early time points. Ultrastructurally, there was no evidence of cardiomyocyte death at 0.5 hour. After three hours, a clear temporal disconnect occurred: lesion scores increased with declining cTnI, cTnT, and H-FABP values. Serum cTns are sensitive and specific markers for detecting acute/active cardiomyocyte injury in this rat model. Heart fatty acid-binding protein is a good early marker but is less sensitive and nonspecific. Release of these biomarkers begins early in myocardial injury, prior to necrosis. Assessment of cTn merits increased consideration for routine screening of acute/ongoing cardiomyocyte injury in rat toxicity studies.

  5. White adipose tissue resilience to insulin deprivation and replacement.

    Directory of Open Access Journals (Sweden)

    Lilas Hadji

    Full Text Available Adipocyte size and body fat distribution are strongly linked to the metabolic complications of obesity. The aim of the present study was to test the plasticity of white adipose tissue in response to insulin deprivation and replacement. We have characterized the changes of adipose cell size repartition and gene expressions in type 1 diabetes Sprague-Dawley rats and type 1 diabetic supplemented with insulin.Using streptozotocin (STZ-induced diabetes, we induced rapid changes in rat adipose tissue weights to study the changes in the distribution of adipose cell sizes in retroperitoneal (rWAT, epididymal (eWAT and subcutaneous adipose tissues (scWAT. Adipose tissue weights of type 1 diabetic rats were then rapidly restored by insulin supplementation. Cell size distributions were analyzed using multisizer IV (Beckman Coulter. Cell size changes were correlated to transcriptional regulation of genes coding for proteins involved in lipid and glucose metabolisms and adipocytokines.The initial body weight of the rats was 465±5.2 g. Insulin privation was stopped when rats lost 100 g which induced reductions in fat mass of 68% for rWAT, 42% for eWAT and 59% for scWAT corresponding to decreased mode cell diameters by 31.1%, 20%, 25.3%, respectively. The most affected size distribution by insulin deprivation was observed in rWAT. The bimodal distribution of adipose cell sizes disappeared in response to insulin deprivation in rWAT and scWAT. The most important observation is that cell size distribution returned close to control values in response to insulin treatment. mRNAs coding for adiponectin, leptin and apelin were more stimulated in scWAT compared to other depots in diabetic plus insulin group.Fat depots have specific responses to insulin deprivation and supplementation. The results show that insulin is a major determinant of bimodal cell repartition in adipose tissues.

  6. 血清补体C3和C4对慢性乙型肝炎患者肝组织病理状态的判断价值%Serum complement C3 and C4 levels for evaluation of hepatic pathological status in patients with chronic hepatitis B

    Institute of Scientific and Technical Information of China (English)

    张占卿; 陆伟; 王雁冰; 饶敏; 冯洁; 沈芳; 冯艳玲

    2011-01-01

    AIM: To explore the possibility of using serum complement C3 and C4 levels to estimate the hepatic pathological status in patients with chronic hepatitis B.METHODS: Four hundred and fifty-six patients with pathologically proven chronic hepatitis B were enrolled in present study. Serum complement C3 and C4 levels were determined using the Beckman-Coulter Immage 800 im-munochemistry system and auxiliary reagents (Beckman-Coulter Inc., USA). Bayes stepwise discriminant analysis was used for evaluation of serum complement C3 and C4 in discriminating hepatic pathological grade and stage.RESULTS: Only serum C3 or C4 entered the Fisher's discriminant functions for discriminating hepatic pathological grade and stage according to the entry criteria of the independents (Wilks' λ = 0.967, x2 = 15.312, P = 0.002 and Wilks' λ = 0.938, x2 = 28.724, P = 0.000). The correctly classified rates of the originally grouped cases by the discriminant functions built with serum complement C3 and C4 were 46.34% for G1, 6.28% for G2, 20.61% for G3, 72.73% for G4 and 6.90% for SO, 46.51% for S1, 7.58% for S2, 8.91% for S3, 61.11% for S4, respectively. According to the discriminant functions for discriminating different pathological grades and stages, patients had a higher probability of Gl and G4 when serum complement C3 level was no less than 0.84 g/L and no more than 0.65 g/L, respectively, and of SI and S4 when serum complement C4 level was no less than 0.18 g/L and no more than 0.14 g/L respectively.CONCLUSION: Serum complement C3 and C4 levels have appreciable significance in discriminating hepatic pathological grade and stage in patients with chronic hepatitis B.%目的:探讨血清补体C3 和C4判别慢性乙型肝炎患者肝组织病理学分级和分期的价值.方法:456 例经肝组织病理学检查的慢性乙型肝炎患者入选本研究.血清补体C3 和C4采用Beckman-Coulter Immage 800 免疫化学系统及其配套试剂测定.血清补体C3 和C4判别病理学分

  7. 血清补体C3和C4预测慢性乙型肝炎肝组织病理学状态的价值%Value of serum complement C3 and C4 for predicting hepatic pathological status in patients with chronic hepatitis B

    Institute of Scientific and Technical Information of China (English)

    张占卿; 陆伟; 饶敏; 王雁冰; 陈淑琴; 童海涛; 沈芳

    2011-01-01

    Objective To explore the clinical value of serum complement C3 and C4 for predicting the hepatic inflammatory activities and fibrotic levels in patients with chronic hepatitis B. Methods 416 patients with chronic hepatitis B with pathological diagnoses of liver tissue were enrolled in present study. Serum complement C3 and C4 were determined by Beckman-Coulter Immage 800 immunochemistry system and auxiliary reagents( Beckman-Coulter Inc. , USA). Results The areas under ROC curve of serun complement C3 and C4 for predicting significant inflammation( ≥G2 ) ,moderate inflammation( ≥G3 ),severe inflammation ( = G4 ) were respectively 0.602 (95% CI:0. 545-0. 659) and 0.550 (95 % CI:0.4890. 611 ), 0.620 ( 95% CI: 0. 559-0. 681 ) and 0. 606 ( 95% CI: 0.547-0. 666 ), 0. 803 ( 95% CI: 0. 6700. 937 ) and 0.654 (95 % CI:0. 486-0. 823 ); and those for predicting significant fibrosis ( ≥ S2 ), severe fibrosis( ≥S3 ) ,cirrhosis( = S4 ) were respectively 0. 590(95% CI:0. 531-0. 649) and 0. 582 (95% CI:0. 519-0. 644) ,0. 665 (95% CI:0. 612-0. 718) and 0. 655 (95% CI:0. 602-0. 707 ), 0. 669 (95% CI:0. 603-0. 736) and 0. 670(95% CI :0. 607-0. 734). The optimal cut-offs of serum complement C3 and C4 for predicting severe inflammation and cirrhosis were respectively ≤0.58 g/L and ≤0.12 g/L, and the corresponding sensitivity, specificity, accuracy were 0. 750 and 0. 480,0. 797 and 0. 792, 0. 796 and 0. 719. Conclusion Serum complement C3 and C4 have some value for predicting respectively hepatic severe inflammation and cirrhosis in patients with chronic hepatitis B.%目的 探讨血清补体C3和C4预测慢性乙型肝炎肝组织炎症活动度和纤维化程度的价值.方法 416例经肝组织病理学检查的慢性乙型肝炎患者入选本研究.血清补休C3和C4采用Beckman-Coulter Immage 800免疫化学系统及其配套试剂测定.结果 血清补体C3和C4预测显著炎症(≥G2)、中度炎症(≥G3)、重度炎症(=G4)

  8. Evaluation of Oxacillin and Cefoxitin Disk and MIC Breakpoints for Prediction of Methicillin Resistance in Human and Veterinary Isolates of Staphylococcus intermedius Group.

    Science.gov (United States)

    Wu, M T; Burnham, C-A D; Westblade, L F; Dien Bard, J; Lawhon, S D; Wallace, M A; Stanley, T; Burd, E; Hindler, J; Humphries, R M

    2016-03-01

    Staphylococcus pseudintermedius is a coagulase-positive species that colonizes the nares and anal mucosa of healthy dogs and cats. Human infections with S. pseudintermedius range in severity from bite wounds and rhinosinusitis to endocarditis; historically, these infections were thought to be uncommon, but new laboratory methods suggest that their true incidence is underreported. Oxacillin and cefoxitin disk and MIC tests were evaluated for the detection of mecA- or mecC-mediated methicillin resistance in 115 human and animal isolates of the Staphylococcus intermedius group (SIG), including 111 Staphylococcus pseudintermediusand 4 Staphylococcus delphini isolates, 37 of which were mecA positive. The disk and MIC breakpoints evaluated included the Clinical and Laboratory Standards Institute (CLSI) M100-S25 Staphylococcus aureus/Staphylococcus lugdunensis oxacillin MIC breakpoints and cefoxitin disk and MIC breakpoints, the CLSI M100-S25 coagulase-negative Staphylococcus (CoNS) oxacillin MIC breakpoint and cefoxitin disk breakpoint, the CLSI VET01-S2 S. pseudintermedius oxacillin MIC and disk breakpoints, and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) S. pseudintermedius cefoxitin disk breakpoint. The oxacillin results interpreted by the VET01-S2 (disk and MIC) and M100-S25 CoNS (MIC) breakpoints agreed with the results of mecA/mecC PCR for all isolates, with the exception of one false-resistant result (1.3% of mecA/mecC PCR-negative isolates). In contrast, cefoxitin tests performed poorly, ranging from 3 to 89% false susceptibility (very major errors) and 0 to 48% false resistance (major errors). BD Phoenix, bioMérieux Vitek 2, and Beckman Coulter MicroScan commercial automated susceptibility test panel oxacillin MIC results were also evaluated and demonstrated >95% categorical agreement with mecA/mecC PCR results if interpreted by using the M100-S25 CoNS breakpoint. The Alere penicillin-binding protein 2a test accurately detected all

  9. TSH-CHECK-1 test: diagnostic accuracy and potential application to initiating treatment for hypothyroidism in patients on anti-tuberculosis drugs.

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    Cara S Kosack

    Full Text Available BACKGROUND: Thyroid-stimulating hormone (TSH promotes expression of thyroid hormones which are essential for metabolism, growth, and development. Second-line drugs to treat tuberculosis (TB can cause hypothyroidism by suppressing thyroid hormone synthesis. Therefore, TSH levels are routinely measured in TB patients receiving second-line drugs, and thyroxin treatment is initiated where indicated. However, standard TSH tests are technically demanding for many low-resource settings where TB is prevalent; a simple and inexpensive test is urgently needed. METHODS: As a proof of concept study TSH was measured in routinely collected sera at the University Medical Center Utrecht, Netherlands, using the TSH-CHECK-1 (VEDALAB, Alençon, France, a lateral-flow rapid immunochromatographic assay with a TSH cut-off value of 10 µIU/mL, the standard threshold for initiating treatment. These results were compared with TSH levels measured by a reference standard (UniCel DXi 800 imunoassay system, Beckman Coulter, USA. Sensitivity, specificity, and likelihood ratios were then calculated. RESULTS: A total of 215 serum samples were evaluated: 107 with TSH values <10 µIU/mL and 108 with values ≥10 µIU/mL. TSH-CHECK-1 test sensitivity was found to be 100.0% (95% CI: 96.6-100.0 and specificity was 76.6% (95% CI: 67.5-84.3. Predictive values (PV were modelled for different levels of prevalence. For a prevalence of 10% and 50%, the positive PV was 32.2% (95% CI: 25.0-39.7% and 81.1% (95% CI: 75.0-85.5%, respectively; the negative PV was 100% (95% CI: 98.9-100% and 100% (95% CI: 91.3-100% respectively. DISCUSSION/CONCLUSIONS: The TSH-CHECK-1 rapid test was practical and simple to perform but difficult to interpret on weak positive results. All sera with TSH≥10 µIU/mL were correctly identified, but the test lacked sufficient specificity. Given its excellent negative PV in this evaluation, the test shows promise for ruling out hypothyroidism. However, so far it

  10. Pseudoleukocytosis secondary to hepatitis C-associated cryoglobulinemia: a case report

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    Geara Abdallah

    2009-11-01

    Full Text Available Abstract Introduction Laboratory tests play a central role in assessing a patient and orienting the diagnostic evaluation. We report a case where the discrepancy between the manual and automatic cell count gave a hint to the final diagnosis. Case presentation A 55-year-old Caucasian man, known to have hepatitis C, was admitted with acute respiratory failure secondary to acute pulmonary edema and diffuse petechial rash of the lower extremities for the previous 2 months. The initial laboratory tests showed acute renal failure (creatinine of 2.6 mg/dL. During his hospital stay, the patient had a fluctuating white blood cell count with a recorded value of 96,000 cells/mL. On a peripheral smear, the blood cell count was in the normal range. The acute renal failure was secondary to membranoproliferative glomerulonephritis secondary to essential mixed cryoglobulinemia diagnosed by biopsy. The complete blood count values, performed by Beckman/Coulter GenS, were falsely high due to precipitation of plasma cryoglobulins at room temperature. This spurious leukocytosis was previously described in several case reports, but values as high as 96,000 cells/mL were never reported. Conclusion The presence of cryoglobulins in the blood creates a clinical challenge for the interpretation of several laboratory tests. Pseudoleukocytosis secondary to cryoglobulinemia has been observed in several reported cases with white blood cell counts up to 54,000 cells/mL at room temperature and 85,600 cells/mL at 4°C. If the cryoglobulin precipitates rapidly, aggregated cryoglobulin particles may be interpreted as blood cells. We report the first patient with pseudoleukocytosis secondary to hepatitis C cryoglobulinemia with a spurious leukocytosis of 96,000 cells/mL at room temperature. Other laboratory tests could also be affected: underestimation of true erythrocyte sedimentation rate, pseudothrombocytosis and pseudolymphocytosis. The precipitation can remove the hepatitis C

  11. Research of complement activity in children with anaphylactoid purpura%儿童过敏性紫癜补体活性研究

    Institute of Scientific and Technical Information of China (English)

    肖珊珊; 刘丽晓; 张佳慧; 范慧子

    2012-01-01

    Objective To investigate the complement C3, C4 activity in children with anaphylactoid purpura of acute and convalescent phase. Methods 97 children with anaphylactoid purpura treated in our hospital from October 2009 to November 2011 were selected as research objects. According to the clinical manifestation, all patients were divided into the skin-type group, abdominal-type group, kidney-type group, joint-type group and mixed-type group. According to the progression disease, patients were divided into the acute phase and the convalescent phase. At the same time, the complement levels were compared with that 32 cases of healthy children, who were taken as the control group. Then the children' serum C3, C4 level were detected by immune scattering rate turbidimetric method using Array 360 scattering immune turbidimetric instrument produced in Beckman-coulter company. Results In the acute and convalescent phase of anaphylactoid purpura children, the serum C3 and C4 levels among different clinical type groups had no difference (P > 0.05). The serum C4 level in acute, convalescent phase groups and control group had no statistically significant difference (P > 0.05); but compared with the control group, the C3 level in acute phase reduced (P 0.05);急性期、恢复期与对照组相比,血清补体C4水平差异无统计学意义(P > 0.05);与对照组相比,急性期血清补体C3水平降低(P < 0.05),恢复期C3水平相对急性期升高,但仍较对照组低(P < 0.05).结论 不同临床分型过敏性紫癜患儿其血清补体水平无明显差异;而相对于健康患儿其急性期补体C3水平降低,恢复期相对回升,而血清补体C4水平无变化.

  12. Reference Intervals of Serum Complement C3and C4 for 1 to 8 Years Old Healthy Children in Panzhihua Area%攀枝花地区1~8岁健康儿童补体C3、C4含量检测及其参考范围的建立

    Institute of Scientific and Technical Information of China (English)

    严达刚; 段春燕; 董玉红; 杨宝国

    2013-01-01

    Objective To establish the reference intervals of serum complement C3 ad C4 for 1 to 8 years old healthy children in Pan-zhihua area. Methods Immunoturbidimetry was applied in detecting serum complement C3 and C4 of 912 children of 1 to 8 years old with IMMAGE 800(Beckman-coulter company, USA)special protein analyzer;the results of 7 age stages were statistical analyzed. Re-sults The difference of sex and age in serum complement C3 and C4 among 1 to 8 years old healthy children in Panzhihua area was of no statistical significance(P>0.05), the reference intervals were as follows:C3:0.60~1.82g/L, C4:0.18~0.22g/L, which was of statisti-cal difference from those in“The National Clinical Test Regualation of Operation”and the reference intervals provided by the manufac-turers(P0.05),总的参考范围为C3:0.60~1.82g/L,C4:0.18~0.22g/L。同时与全国临床检验操作规程及厂家提供的参考范围相比较,差异均有统计学意义(P<0.05)。结论全国临床检验操作规程及厂家提供的参考范围已不适应本地区1~8岁儿童血清补体C3、C4的参考范围。各地各实验室应建立各自实验室的参考范围;若存在性别、年龄组间差异,应分别制定性别、年龄各段各自的参考范围。

  13. Evaluation of Oxacillin and Cefoxitin Disk and MIC Breakpoints for Prediction of Methicillin Resistance in Human and Veterinary Isolates of Staphylococcus intermedius Group.

    Science.gov (United States)

    Wu, M T; Burnham, C-A D; Westblade, L F; Dien Bard, J; Lawhon, S D; Wallace, M A; Stanley, T; Burd, E; Hindler, J; Humphries, R M

    2016-03-01

    Staphylococcus pseudintermedius is a coagulase-positive species that colonizes the nares and anal mucosa of healthy dogs and cats. Human infections with S. pseudintermedius range in severity from bite wounds and rhinosinusitis to endocarditis; historically, these infections were thought to be uncommon, but new laboratory methods suggest that their true incidence is underreported. Oxacillin and cefoxitin disk and MIC tests were evaluated for the detection of mecA- or mecC-mediated methicillin resistance in 115 human and animal isolates of the Staphylococcus intermedius group (SIG), including 111 Staphylococcus pseudintermediusand 4 Staphylococcus delphini isolates, 37 of which were mecA positive. The disk and MIC breakpoints evaluated included the Clinical and Laboratory Standards Institute (CLSI) M100-S25 Staphylococcus aureus/Staphylococcus lugdunensis oxacillin MIC breakpoints and cefoxitin disk and MIC breakpoints, the CLSI M100-S25 coagulase-negative Staphylococcus (CoNS) oxacillin MIC breakpoint and cefoxitin disk breakpoint, the CLSI VET01-S2 S. pseudintermedius oxacillin MIC and disk breakpoints, and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) S. pseudintermedius cefoxitin disk breakpoint. The oxacillin results interpreted by the VET01-S2 (disk and MIC) and M100-S25 CoNS (MIC) breakpoints agreed with the results of mecA/mecC PCR for all isolates, with the exception of one false-resistant result (1.3% of mecA/mecC PCR-negative isolates). In contrast, cefoxitin tests performed poorly, ranging from 3 to 89% false susceptibility (very major errors) and 0 to 48% false resistance (major errors). BD Phoenix, bioMérieux Vitek 2, and Beckman Coulter MicroScan commercial automated susceptibility test panel oxacillin MIC results were also evaluated and demonstrated >95% categorical agreement with mecA/mecC PCR results if interpreted by using the M100-S25 CoNS breakpoint. The Alere penicillin-binding protein 2a test accurately detected all

  14. Measurement of particle size distribution of soil and selected aggregate sizes using the hydrometer method and laser diffractometry

    Science.gov (United States)

    Guzmán, G.; Gómez, J. A.; Giráldez, J. V.

    2010-05-01

    Soil particle size distribution has been traditionally determined by the hydrometer or the sieve-pipette methods, both of them time consuming and requiring a relatively large soil sample. This might be a limitation in situations, such as for instance analysis of suspended sediment, when the sample is small. A possible alternative to these methods are the optical techniques such as laser diffractometry. However the literature indicates that the use of this technique as an alternative to traditional methods is still limited, because the difficulty in replicating the results obtained with the standard methods. In this study we present the percentages of soil grain size determined using laser diffractometry within ranges set between 0.04 - 2000 μm. A Beckman-Coulter ® LS-230 with a 750 nm laser beam and software version 3.2 in five soils, representative of southern Spain: Alameda, Benacazón, Conchuela, Lanjarón and Pedrera. In three of the studied soils (Alameda, Benacazón and Conchuela) the particle size distribution of each aggregate size class was also determined. Aggregate size classes were obtained by dry sieve analysis using a Retsch AS 200 basic ®. Two hundred grams of air dried soil were sieved during 150 s, at amplitude 2 mm, getting nine different sizes between 2000 μm and 10 μm. Analyses were performed by triplicate. The soil sample preparation was also adapted to our conditions. A small amount each soil sample (less than 1 g) was transferred to the fluid module full of running water and disaggregated by ultrasonication at energy level 4 and 80 ml of sodium hexametaphosphate solution during 580 seconds. Two replicates of each sample were performed. Each measurement was made for a 90 second reading at a pump speed of 62. After the laser diffractometry analysis, each soil and its aggregate classes were processed calibrating its own optical model fitting the optical parameters that mainly depends on the color and the shape of the analyzed particle. As a

  15. Severe malnutrition with and without HIV-1 infection in hospitalised children in Kampala, Uganda: differences in clinical features, haematological findings and CD4+ cell counts

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    Downing Robert

    2006-10-01

    Full Text Available Abstract Background The aim of this study was to describe the clinical features, haematological findings and CD4+ and CD8+ cell counts of severely malnourished children in relation to human immunodeficiency virus (HIV infection. Methods The study was conducted in the paediatric wards of Mulago hospital, which is Uganda's national referral and teaching hospital. We studied 315 severely malnourished children (presence of oedema and/or weight-for-height: z-score + and CD8+ cells were measured by the flow cytometry and HIV serology was confirmed by Enzyme linked Immunoassay for children >18 months of age, and RNA PCR was performed for those ≤18 months. Complete blood count, including differential counts, was determined using a Beckman Coulter counter. Results Among the 315 children, 119 (38% were female; the median age of these children was 17 months (Interquartile range 12–24 months, and no difference was observed in the HIV status with regard to gender or age. The children showed a high prevalence of infections: pneumonia (68%, diarrhoea (38%, urinary tract infection (26% and bacteraemia (18%, with no significant difference with regard to the HIV status (HIV-positive versus HIV-negative children. However, the HIV-positive children were more likely to have persistent diarrhoea than the HIV-uninfected severely malnourished children (odds ratio (OR 2.0, 95% confidence interval (CI 1.2–3.6. When compared with the HIV-negative children, the HIV-positive children showed a significantly lower median white blood cell count (10700 versus 8700 and lymphocyte count (4033 versus 2687. The CD4+ cell percentages were more likely to be lower in children with non-oedematous malnutrition than in those with oedematous malnutrition even after controlling for the HIV infection. The novel observation of this study is that the CD4+ percentages in both HIV-positive and HIV-negative children without oedema were lower that those in children with oedema. These

  16. False elevation of cardiac markers: importance of recognition

    Directory of Open Access Journals (Sweden)

    Yaser Elnahar

    2011-03-01

    Full Text Available Yaser Elnahar, Joseph Daoko, Karim El Kersh, Jennifer C Kam, Chady Sarraf, Fayez ShamoonSt. Michael’s Medical Center, Newark, NJ, USAAbstract: The availability of troponins as cardiac markers in the diagnosis of acute coronary syndrome is invaluable. However, their elevation can sometimes lead the physician astray. We report a rare case of an 86-year-old Hispanic female with a past medical history significant for asthma, hypertension, atrial fibrillation, and dyslipidemia, who presented to the emergency room complaining of a two-day history of shortness of breath associated with wheezing. She denied any chest pain. The patient’s wheezing ameliorated with bronchodilator treatment. However, her admission laboratory investigations were positive for elevated troponin I, with normal creatine kinase (CK and CK-myoglobin (MB. The first set of cardiac enzymes revealed a troponin I of 29.16 ng/mL (normal < 0.05 ng/mL, CK 234 IU/L, and CK-MB 3.9 IU/L. The electrocardiogram showed rate-controlled atrial fibrillation with nonspecific ST changes. Subsequent cardiac enzymes failed to show any increase in CK or CK-MB. However, the troponin I was, as on admission, persistently elevated at 20.87–29.16 ng/mL. Subsequent cardiac catheterization revealed mild nonobstructive coronary artery disease. Other laboratory tests showed normal creatinine, alkaline phosphatase, and bilirubin, and a negative rheumatoid factor, with absence of hemolysis. A blood sample was subsequently drawn and sent to Beckman Coulter laboratories for heterophile antibody testing. The results confirmed our suspicion of a falsely elevated troponin I caused by the presence of a heterophile antibody. The addition of blocking agents yielded troponin I levels in the normal range. Consistent with current guidelines, we conclude that cardiac markers should be used in conjunction with the clinical picture and the electrocardiogram. This case is unique in that the troponin elevation was

  17. [The influence of dietary fibers on cell immunity under the adequate nutrition and in the presence of alimentary polyhypovitaminosis in rats].

    Science.gov (United States)

    Trushina, É N; Mustafina, O K; Vrzhesinskaia, O A

    2013-01-01

    The effect of wheat bran on cell immunity in rats adequately provided with vitamins or insufficiently supplied with vitamins has been investigated. 48 male Wistar rats (58.1 +/- 0.5 g) were divided into 6 group and fed with complete semi-synthetic diet, containing 100% or 20% of vitamin mixture (Vit) with or without supplement of insoluble dietary fiber (DF) in the dose corresponding to the upper allowable level of its consumption (5% wheat bran of diet mass) for 4 weeks. The animals of the 1 group received 100% of vitamin mixture (100% Vit); 2 group--100% Vit+DF; 3 group--20% of vitamin mixture (20% Vit); 4 group--20% of vitamin mixture and DF (20% Vit+DF). The next 5 days rats from vitamin-deficient groups were fed with diets supplemented with 80% of Vit: (5 group--20% Vit+80% Vit; 6 group--20% Vit+DF+80% Vit). The contents of lymphocytes, relative quantity of B-(CD45RA+) and T-lymphocytes (CD3+), subpopulations of T-lymphocytes: T-helper (CD3+CD4+) and cytotoxic T-lymphocytes (CD3+CD8+), NK-cells (CD161a+) in the peripheral blood of rats were determined by the method of flow cytometry using Beckman Coulter FC 500 (USA) cytometer. In rats fed complete semi-synthetic diet supplemented with DF (100% Vit+DF) the reduction of relative contents of T-lymphocytes and the increase of the fraction of cytotoxic T-lymphocytes in peripheral blood has been found. The analogous changes and more pronounced degree of immunosupression, that appeared in a lymphocytopenia, much smaller level of T-lymphocytes, T-helper and increase of cytotoxic T-lymphocytes content in rats fed a low vitamins diet (20% Vit) in comparison with these parameters of control group, have been detected. In rats received 20% Vit+DF the suppressed cell immunity was accompanied with decreased level of NK-cells. Normalization of vitamins content in the diets of rat deficient groups led to an almost complete recovery of cell immunity indicators to the level of the animals from the corresponding control groups

  18. Two Species of Myxomycetes Causing Slime Mold of Sweet Potato

    OpenAIRE

    Kim, Wan Gyu; Lee, Sang Yeob; Cho, Weon Dae

    2007-01-01

    Specimens collected from sweet potato plants with slime mold symptoms in fields in Daejeon, Korea were examined. Two species of Myxomycetes, Fuligo septica and Stemonitis herbatica were identified based on their morphological characteristics. This is the first report that the two species of Myxomycetes cause slime mold of sweet potato in Korea.

  19. Clinical evaluation of leukocyte differential count in peripheral blood by five-color flow cytometry%五色流式细胞术分类计数外周血白细胞的临床评价

    Institute of Scientific and Technical Information of China (English)

    邢莹; 王建中; 普程伟; 尚柯

    2013-01-01

    Peking University First Hospital from September 2010 to December 2010 was detected by standard microscopic cytology as a reference method.Meanwhile,Beckman-Coulter LH750 hematology analyzer and FC500 flow cytometer were performed.Then the correlations were analyzed between microscopic cytology,hematology analyzer and flow cytometry.Forty blood samples collected at Peking University First Hospital,Beijing Daopei Hospital and General Hospital of Beijing Military Command from August 2010 to November 2010 were analyzed by standard microscopic cytology,Beckman-Coulter LH750 hematology analyzer and NAVIOS flow cytometer.Then the correlations between microscopy,hematology analyzer and flow cytometry were explored to analyze the clinical diagnostic efficiency of flow cytometry.Results Correlation of leukocyte differential count between FC500 flow cytometer and standard microscopic cytology was significant(all P < 0.01).And it was superior in the detection of lymphocytes,neutrophils and eosinaphils (r =0.955,0.928,0.876).Moreover,the correlation of leukocyte differential count between NAVIOS flow cytometer via manual gate and standard microscopic cytology was significant (r > 0.700,all P < 0.01) except for basophils.And it was superior in the detection of neutrophils,lymphocytes and blasts (r =0.950,0.915,0.852).When 1% was set as the cut-off value of immature granulocytes on standard microscopic cytology,the sensitivity and specificity of flow cytometry was 87% and 76% respectively.When 0.5% was set as the cut-off value of blasts on standard microscopic cytology,the sensitivity and specificity of flow cytometry stood at 100% and 92% respectively.Conclusion Five-color flow cytometry is wellcorrelated with standard microscopic cytology for leukocyte differential count in peripheral blood with different flow cytometers,and the sensitivity of detecting blasts and immature granulocytes is very excellent.

  20. 血清补体C3和C4在预测慢性乙型肝炎肝纤维化程度中的价值%Serum complement C3 and C4 levels for predicting severity of hepatic fibrosis in patients with chronic hepatitis B

    Institute of Scientific and Technical Information of China (English)

    张占卿; 陆伟; 王雁冰; 饶敏; 冯洁; 冯艳玲; 沈芳

    2011-01-01

    目的 探讨血清补体C3和C4预测慢性乙型肝炎肝纤维化程度的价值.方法 选择442例经肝组织病理学检查的慢性乙型肝炎患者.血清补体C3和C4采用Beckman-Coulter Immage 800免疫化学系统及其配套试剂测定.血清补体C3和C4预测肝纤维化程度的评价采用ROC曲线法.结果 血清补体C3和C4预测显著肝纤维化(≥S2)、严重肝纤维化(≥S3)、肝硬化(S4)的ROC 曲线下面积均显著大于标准参考线下面积(P =0.009、0.000、0.000和P=0.005、0.000、0.000).根据ROC曲线,血清补体C3预测严重肝纤维化和肝硬化的最佳截断值分别为≤0.74 g/L和≤0.64 g/L,其对应的灵敏度、特异度、阳性预测值、阴性预测值、准确度分别为0.585、0.681、0.617、0.650、0.636和0.509、0.775、0.423、0.830、0.710;血清补体C4预测严重肝纤维化和肝硬化的最佳截断值分别为≤0.14 g/L和≤0.12 g/L,其对应的灵敏度、特异度、阳性预测值、阴性预测值、准确度分别为0.565、0.634、0.576、0.623、0.602和0.463、0.781、0.407、0.818、0.704.结论 血清补体C3和C4对慢性乙型肝炎严重肝纤维化和肝硬化有一定预测价值,但效能不够稳定和可靠.%Objective To investigate the clinical value of serum complement C3 and C4 levels for predicting the severity of hepatic fibrosis in patients with chronic hepatitis B.Methods Histopathological diagnosis was confirmed in 442 patients with chronic hepatitis B.Serum complement C3 and C4 levels were determined by Beckman-Coulter Immage 800 immunochemistry system.ROC curve was used to analyze the value of serum complement C3 and C4 levels in predicting the severity of hepatic fibrosis.Results The areas under ROC curve of complement C3 and C4 for predicting significant fibrosis ( ≥ S2),severe fibrosis ( ≥ S3) and cirrhosis (S4) were all significantly larger than the area under diagonal reference line ( P =0.009,0.000,0.000 and P =0.005,0.000,0.000,respectively

  1. Determining the Dimension of Knowledge Management Implementation by Utilizing a Fuzzy TOPSIS Method

    OpenAIRE

    Mona Ahani; Hamid Reza Bahrami; Behrooz Shariflu

    2013-01-01

    The purpose of this research is to identify and rank different aspects of knowledge management based on the Beckman model using the Fuzzy TOPSIS technique. The Beckman model, in this research, has 8 main dimensions including: knowledge identification, knowledge acquisition, knowledge selection, knowledge storage, knowledge distribution, knowledge creation, knowledge application, knowledge business, and 33 sub-criteria or alternatives. The population of the present study includes 197 members o...

  2. Comparison of a new rapid plasma reagin card test with the standard rapid plasma reagin 18-mm circle card test and the venereal disease research laboratory slide test for serodiagnosis of syphilis.

    OpenAIRE

    Hambie, E A; Larsen, S A; Perryman, M W; Pettit, D E; Mullally, R L; Whittington, W

    1983-01-01

    The rapid plasma reagin (RPR) card test manufactured by Beckman Instruments, Inc., was compared, qualitatively and quantitatively, with the Venereal Disease Research Laboratory (VDRL) slide test and the standard RPR 18-mm circle card tests for the serodiagnosis of syphilis. Sera from 638 individuals were used in this study. Two pilot lots and two production lots of antigen were submitted by Beckman Instruments, Inc., for evaluation. Qualitative agreement among the three RPR card tests was 98....

  3. Força de tração e volume de solo mobilizado por haste sulcadora em semeadura direta sobre campo nativo, em função do teor de água no solo, profundidade e velocidade de operação Draft and soil loosening by knife type coulter related to soil moisture and planter's working speed and depth

    OpenAIRE

    Carla T. C. Cepik; Carlos R. Trein; Renato Levien

    2005-01-01

    A semeadura direta é um sistema de manejo eficiente em termos de conservação do solo e da água, economia de energia e capacidade operacional dos conjuntos mecanizados. No entanto, em áreas onde existem pecuária e produção de grãos, ou onde o solo é trafegado e trabalhado em condições inadequadas, pode ocorrer compactação do solo nas camadas superficiais. Mesmo compactadas, essas camadas não necessitariam ser descompactadas ou revolvidas, se boa germinação e desenvolvimento da cultura fossem a...

  4. 两台化学发光仪检测人生长激素的比对分析及偏倚评估%Comparative analysis and bias assessment of detecting results of human growth hormone using two chemiluminescence ap-paratus

    Institute of Scientific and Technical Information of China (English)

    杨方华; 邓芳梅; 孙丽芳

    2013-01-01

    Objective To explore the comparability of human growth hormone(hGH) detected by chemilumi-nescence apparatus of SIEM ENS Immulite 1000 and Beckman Coulter DXI 800 detection systems ,through methodol-ogy comparing and bias assessing .Methods According to Clinic and Laboratory Standrd Instritate (CLSI) EP9-A2 document ,at least 8 copies of different concentrations of fresh serum were collected everyday ,then we tested hGH with two detection systems for at least 5 days continuously .Every sample was detected repeatedly with obverse and reverse sequence ,then we recorded the results ,checked outlier ,observed linearity and data distribute ,and calculated correlation coefficient and linear regression equation .According to the regression equation ,we calculated hGH in the critical value of the expected bias and the expected bias of the 95% confidence interval ,and determined the bias ac-cepted or not .Results The correlation coefficient (r) of two detection systems in hGH was 0 .999 ,and the difference of mean results was negative bias .The bias was in the permissible error range ,and it was acceptable .Conclusion Methodology comparison and bias estimation of the same test items in different detection systems should be carried out regularly in order to ensure comparability and continuity of test results .%目的:对 Immulite 1000化学发光仪和 DXI 800化学发光仪两种不同检测系统检测人生长激素(hG H )进行方法比对及偏倚评估,探讨不同检测系统间测定结果是否具有可比性。方法依据美国临床和实验室标准化协会(CLSI)EP9-A2文件要求,每天至少收集8份不同浓度的新鲜血清,分别用两种检测系统测定hGH ,连续测定至少5d,每个标本均按正反序重复测定,记录测定结果,检查离群点,目测线性及数据分布,计算相关系数和直线回归方程,根据回归方程计算hG H在临界值处的预期偏倚和预期偏倚的95%可信区间并判断

  5. 流式细胞术分析小鼠外周血免疫细胞全血裂解试剂比较研究%Comparative Study of Whole Blood Lysis Reagents for Analysis of Immunocytes in Peripheral Blood of Mice by Flow Cytometry

    Institute of Scientific and Technical Information of China (English)

    崔巍; 刘飒; 蔡伦; 李玉琳; 张聪聪; 邱淑兰

    2011-01-01

    This study was purposed to investigate the efficacy of different whole flow lysis reagents for lysis of red blood cells in flow cytometric analysis. The expression of immunocytes was detected by flow cytometry after lysis of red blood cells using commercial reagents (Optilyse C, FACS Lysing Solution) and self-made red blood cell lysis reagents ( RBC Lysis Buffer), the detection results were analyzed comparatively. The results showed that there was no significant difference in the percentege of CD3e+, CD3e + CD4 +, CD3e + CD8a +, CD3e- CD19 +, CD3e- NK1.1 + and Gr-1 +cells between 3 different lysis reagent groups. However OptiLyse C solution was suitable to Gr-1 + cell detection, but did not suit to Foxp3 + Treg detection. The self-made RBC Lysis Buffer and FACS Lysing Solution were suited to Foxp3 +Treg detection. It is concluded that the use of self-made RBC Lysis Buffer for flow cytometry can get the lysis efficiency of commercially avaiblable lysis solutions when samples are prepared in accordance with standarized procedure. The selfmade RBC Lysis Buffer not only can satisfy experimental requirements, but also can reduce the experimental costs.%本研究探讨流式细胞术外周血样本制备中红细胞裂解液的作用.用流式细胞术检测进口的商品化红细胞裂解液OptiLyse C(Beckman Coulter),FACS Lysing Solution(BD Pharmingen)以及自制裂解液RBC Lysis Buffer裂解小鼠外周血红细胞后免疫细胞的表达情况并进行了对比分析.结果表明,用3种裂解液制备样本中CD3e+、CD3e+CD4+、CD3e+CDSa+、CD3e-CD19+、CD3e-NK1.1+和Gr-1+细胞的百分比均无明显差异(p>0.05);而OptiLyse C裂解液更适于Gr-1+细胞测定,不适于Foxp3+Tregs细胞测定;FACS Lysing Solution和自制RBC Lysis Buffer适于Foxp3+Tregs细胞测定.结论:应用自制RBC Lysis Buffer裂解液,按标准化操作程序制备流式样本,能够达到进口的商品化裂解液OptiLyse C的效果,它不仅能满足实验要求,又可降低试剂成本,可推广应用.

  6. Correlation between level of serum prolactin and epileptic discharges of electroencephalogram from 24 to 36 hours after epileptic onset

    Institute of Scientific and Technical Information of China (English)

    Xiaowei Hu; Wanli Dong; Min Xu

    2006-01-01

    BACKGROUND: Researchers discovered that serum prolactin could rise following an epileptic seizure. The prolactin level might reach three times more than basic level within 30 minutes and decrease to the normal value 2 hours after the seizure occurred. The mechanism might result in an increase of serum prolactin concentrations with the activation of the hypothalamic-pituitary axis.OBJ ECTIVE:To probe into the correlation between changes of serum prolactin and incidence of epileptic discharges of electroencephalogram (EEG) at 24-36 hours after epileptic onset of patients with secondary epilepsy.DESIGN: Clinical observational study.SETTING: Department of Neurology, First Hospital affiliated to Soochow University.PARTICIPANTS: A total of 21 patients with secondary epilepsy were selected from the Department of Neurological Emergency or Hospital Room of the First Hospital affiliated to Soochow University from November 2005 to April 2006. There were 14 males and 7 females aged from 25 to 72 years. All patients met International League Anti-epileptic (ILAE) criteria in 1981 for secondary generalized tonic clonic seizure through CT or MRI and previous EEG. All patients were consent. Primary diseases included cerebral trauma (3 cases),tumor (2 cases), stroke (7 cases) and intracranial infeion (9 cases).METHODS: Venous blood of all patients was collected at 24-36 hours after epileptic onset. Serum prolactin kit (Beckman Coulter, Inc in USA) was used to measure value of serum prolactin according to kit instruction.Then, value of serum prolactin was compared with the normal value (male: 2.64-13.13 mg/L; female: 3.34-26.72 mg/L); meanwhile, EEG equipment (American Nicolet Incorporation) was used in this study.MAIN OUTCOME MEASURES: ① Abnormal rate of serum prolactin of patients with secondary epilepsy; ②Comparison between normal and abnormal level of serum prolactin and incidence of EEG epileptic discharge of patients with secondary epilepsy.RESULTS :All 21 patients with

  7. Elevated levels of beta-human chorionic gonadotropin and human placental lactogen between 11-13 week's gestation and subsequent pregnancy complications in Oman i women

    International Nuclear Information System (INIS)

    The association between abnormal levels of maternal serum b-human chorionic gonadotrophin (b-HCG) and human placental lactogen (HPL) measured in early pregnancy and future poor pregnancy outcome is fairly well established. Little is known on how such prognostic information can be used to avoid future complications of pregnancy and improve their outcome. We undertook a prospective study, which was designed to assess the efficiency of maternal serum biochemical markers b-HCG and HPL for the detection of different pregnancy and labor complications. The blood samples were taken from pregnant patients who attended the antenatal clinic at Sultan Qaboos University Hospital in Muscat, Sultanate of Oman, from December 2001 to October 2002. These patients were selected randomly. A total of 200 Omani women, none diabetic, with singleton pregnancies between 11 and 13 weeks gestation were recruited into the study. Gestational age was calculated from the first day of the last menstrual period, unless ultrasonography showed a discrepancy of more than 14 days. Excluded from the study were pregnancies with fetal anomalies, multiple gestations and insulin dependent maternal diabetes. Biochemical analysis of both maternal serum markers was performed in the clinical biochemistry laboratory using an automated immunometric technique supplied by Beckman Coulter for b-HCG and manual radioimmunoassay utilizing a gamma radiation scintillation counter for HPL. Both assays underwent internal, trilevel quality controls. The selected patients were monitored for the following complications: pregnancy induced hypertension (PIH), gestational diabetes, polyhydramnios, antepartum hemorrhage, intrauterine growth retardation (IUGR) (birth weight <10th per centile for gestation), low Apgar score (7 or less) and emergency cesarean section. 75 patients did not develop any pregnancy complications and delivered vaginally. This was our control group. 85 patients developed one of the above mentioned problems

  8. Rome

    Directory of Open Access Journals (Sweden)

    Jan Nelis

    2010-01-01

    Full Text Available Apted, Michael; Farino, Julia; Coulter, Allen et al. (dirs.; HBO/BBC (prod.; Heller, Bruno; Milius, John; Frankel, David; Macdonald, William J.; Cunningham, Alexandra and Hodges, Adrian (script. United Kingdom, 2005: son., col.

  9. ECOHAB: Kamykowski_D- Florida Process Cruise 11/16-19/1998- Karenia brevis counts, biochemistry and behavior (NODC Accession 0000533)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Abstract: Water bottle samples were collected from selected depths (variable with station) at 44 stations in the study area. Coulter counts in the 14 to 28 um size...

  10. ECOHAB: Kamykowski_D- Florida Process Cruise 11/16-19/1998- Karenia brevis counts, biochemistry and behavior (NODC Accession 0000534)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Abstract: Water bottle samples were collected from selected depths (variable with station) at 14 stations in the study area. Coulter counts in the 14-28 um size...

  11. A device for loading coal onto a conveyor

    Energy Technology Data Exchange (ETDEWEB)

    Kartavykh, G.I.; Startsev, V.I.

    1980-04-05

    The device contains sliding bearings mounted in the guides under the chute flight of the conveyor which are hinged to the coulters, and a control jack. In order to increase the effectiveness of the process of loading the coal onto the conveyor, the device is equipped with cleaners that are hinged by one end to the chute flight, and the other ends are mounted to allow for interaction with the coulter, and coulter rotation limiters that are built in the form of chains connected to the sliding bearings, and breakup blades with guides that hold the sliding bearings. The control jack cylinder is connected to the chute flight, while the rod is hinged to the coulter.

  12. Karussellile väljavalitud / Aita Kivi

    Index Scriptorium Estoniae

    Kivi, Aita, 1954-

    2000-01-01

    Sisu : Eeva Park. Pääse karussellile; Waris Dirie, Cathleen Miller. Kõrbelill; Colette Davenat. Väljavalitu; Arthur Hailey. Kõrged ametikohad; Mari Saat. Sinikõrguste tuultes; Kogumik "Inimese vägi"; Catherine Coulter. Elu ja surma piiril

  13. 76 FR 45471 - Energy Efficiency Standards for Distribution Transformers; Notice of Intent To Negotiate Proposed...

    Science.gov (United States)

    2011-07-29

    ... distribution transformers (``final rule''). 72 FR 58190-58241 (October 12, 2007). The standards in that final... Systems Jerry Corkran Prolec GE Greg Coulter National Electrical Manufacturers Association Jim...

  14. Effect of Replacement of New Reverse Osmosis Film of Water Purification System to Clinical Biochemistry%实验室纯水系统更换反渗透膜对生化检验的影响

    Institute of Scientific and Technical Information of China (English)

    莫基浩; 刘鲜茹; 李少侠

    2014-01-01

    目的:研究实验室纯水系统更换新反渗透膜后,所产纯水的质量是否符合生化检验的要求并评价其影响。方法通过Beckman Coulter生化分析系统配套纯水机天创TCHS-RO/100A自带电阻表和 HM COM-100电导率测量笔连续监测新产纯水中的实时离子含量。用正常纯水在 AU680上重复20次测量 ALT,AST,TBIL,ALP,GGT,TP,ALB,BUN, CR,UA,GLU,TC,TG,HDL,LDL,CK,LDH,P,Ca(对照组),更换为新产纯水后进行相同试验操作(试验组),以评估新产纯水对生化分析的影响。连续5天,每天3次(间隔2 h)监测新产纯水的 TG试剂空白,监测水中甘油水平。结果开机30 min后,HM COM-100测得电导率降至(0.1±0.0)μS/cm,机载电阻表测得新产纯水在开机50 min后稳定在18.25 MΩcm。在用 AU680进行生化分析时,对照组中TG测量值=1.04 mmol/L,试验组=21.39 mmol/L,差异有统计学意义(P<0.01),而除TG其他生化项目偏倚均在5%以内,CV<1/4CLIA’88,差异无统计学意义(P>0.05)。TG试剂空白在第5天稳定在A660nm=0.0156±0.0004。结论新纯水系统在生产约2800 L水后,所产纯水中的甘油可以清除干净。实验室纯水系统在更换反渗透膜后,需要对水质进行连续监测,以评价其对常规生化项目检测的影响。%Objective To test if the quality of produced water could come up to the standards of clinical biochemistry,and e-valuate the effect of the produced water after replacement of a new reverse osmosis film of the laboratory water purification system.Methods The ion concentration of the produced water was tested by ohmmeter in Beckman Coulter supporting TianChuang water purification system TCHS-RO/100A and HM COM-100 conductivity meter.The biochemical index ALT,AST,TBIL,ALP,GGT,TP,ALB,BUN,CR,UA,GLU,TC,TG,HDL,LDL,CK,LDH,P,Ca of mixed serum were tested 20 times by AU680 under the condition of

  15. 碱性磷酸酶对肌钙蛋白Ⅰ测定干扰的评价%Interference of alkaline phosphatase on clinical cardiac troponin Ⅰ assay

    Institute of Scientific and Technical Information of China (English)

    刘泽金; 郑芳; 张真路; 范庆坤; 王纯

    2010-01-01

    目的 评价ALP对cTnI测定的干扰.方法 制备1份正常混合血浆和2份不同浓度cTnI的异常混合血浆,各分成8组,第1组为对照组,另7组加入不同浓度ALP,分别在ACCESS2 (Beckman Coulter公司)及AXSYM(美国Abbott公司)上测定每组标本的cTnI和ALP,并与对照组cTnI比较,评价ALP是否对cTnI测定产生干扰.结果 ACCESS cTnI测定正常血浆时,ALP浓度高达3 716 U/L时测定结果为(0.04±0.01)μgL,与正常血浆组的(0.04±0.01)μg/L比较差异无统计学意义(t=0.40,P>0.05);ALP高于917 U/L时,AXSYM cTnI测定结果为(0.08±0.01)μg/L,高于正常血浆组的(0.04±0.01)μg/L,差异有统计学意义(t=-4.89,P<0.01);ACCESS cTnI测定异常血浆时,ALP浓度高达3 534 U/L时,cTnI测定结果为(13.41±0.17)μg/L,与正常血浆组的(13.48±0.16)μg/L比较,差异无统计学意义(t=0.52,P>0.05).结论 高浓度ALP对ACCESS cTnI测定无干扰,不会导致假阳性结果,但高浓度ALP(917 U/L以上)会造成AXSYM cTnI检测干扰导致假阳性结果.%Objective To evaluate the interference of ALP on cTnI assays. Methods One normal mixed plasma sample and 2 abnormal mixed plasma samples with different cTnI levels were prepared, and then divided them into 8 groups respectively. One group was randomly chosen as control while different amounts of ALP were added into the other seven groups. The concentrations of cTnI and ALP in each plasma portion were detected by ACCESS2 (Beckman-Coulter, Inc ) and AXSYM (Abbott Laboratories )separately. The results of the seven tested groups were then compared with those of the control, so as to evaluate whether ALP could interfere with the cTnI assay. Results When the chemiluminescent Access cTnI assay was carried out for detection of normal plasma, the concentration of ALP was up to 3 716 U/L and did not interfere with the test results of cTnI [(0. 04 ±0.01) μg/L] compared with those of the control portion [(0. 04 ± 0. 01 ) μg/L] (t = 0. 40, P > 0. 05 ). Once

  16. Setí obilovin a faktory ovlivňující přesnost uložení semen do půdy

    OpenAIRE

    Jungwirthová, Zuzana

    2013-01-01

    The aim of my diploma thesis was to ascertain the applicability of the test drill coulter for precision sowing of cereals and other crop plants. The thesis summarizes the requirements on the drill coulter which could be used for precision sowing of cereals in the future. A part of the study defines the terms associated with the individual sowing characteristics, including a historical overview of the methods of sowing cereals and other crop plants. The study further describes the factors b...

  17. Ekoodlaren

    OpenAIRE

    Tobiasson, Mats

    1992-01-01

    The Ekoodlaren (Eco cropper) is a new implement constructed to prepare the seedbed, to seed, to fertilize and to row weed in single or combined operations. The main purposes for the construction is to save time and money, and to reduce excessive packing of the soil. Its drilling and row hoeing qualities were examined at the Department of Soil Sciences in three series of field trials during 1991. Two of the series, 'Seed coulters: Different tillage methods' (Rl-5015) and 'Seed coulters: Minimi...

  18. Clinical Significance of Detection of Serum Total IgE and Eosinophils in Allergic Skin Diseases%血清总IgE和嗜酸性粒细胞检测在过敏性皮肤病中的临床意义

    Institute of Scientific and Technical Information of China (English)

    易斌; 曾俊萍

    2011-01-01

    目的 探讨血清总IgE及WS水平在过敏性皮肤病中的分布及临床意义.方法 采用HITACHI 7170A全自动生化分析仪和BECKMAN COULTER GEN.S全自动血细胞分析仪分别检测100例过敏性皮肤病患者血清总IgE和血液EOS水平,并对结果进行统计分析.结果 100例过敏性皮肤病患者中血清总IgE水平(阳性率)分别为:过敏性紫癜组(453.1±283,3) IU/ml(12/31,38.71%),湿疹组(531.3±224.5)IU/mI(16/20,80.00%),药疹组(489.7±285.2)IU/ml (9/19,47.37%),尊麻疹组(476.1±211.1) IU/ml (7/15,46.67%),接触性皮炎组(514.0±263.6)IU/ml(9/15,60.00%);血液EOS水平(阳性率)分别为:过敏性紫癜组(0.31±0.55) ×10~9/L(6/31,19.35%),湿疹组(1.17±1.60) ×10~9/L(11/20,55.00%),药疹组(0.48±0.48) × 10~9/L(6/19,31.58%),荨麻疹组(0.46±0.52) ×10~9/L(4/15,26.67%),接触性皮炎组(0.57±0.73)× 10~9/L(5/15,33.33%),除过敏性紫癜组外,湿疹组、药疹组、荨麻疹组以及接触性皮炎组血清总IgE及EOS水平均显著高于正常对照组(P<0.05),但各组IgE值与EOS计数在统计学上均未发现明显相关性( -0.54COULTER GEN. S automatic blood cell analyzer, and then the results were analyzed and compared by statistical methods. Results The sertn total IgE concentrations of 100 patients with allergic skin diseases (positive rate) were as follows: 453.1 ± 283.3 IU/ml (12/31,38.71% ) in allergic purpura group, 531.3

  19. Detection and Quantification of Rotenoids from Clitoria fairchildiana and its Lipids Profile.

    Science.gov (United States)

    Santos, Rauldenis A F; David, Jorge M; David, Juceni P

    2016-05-01

    This work describes the isolation and quantification of rotenoids from crude organic extracts of different parts of Clitoria fairchildiana R. A. Howard (Leguminosae) by HPLC-DAD. The lipid composition and the Artemia salina cytotoxic activities of the isolates were also conducted. Clitoriacetal (1), 6-deoxyclitoriacetal (2), stemonal and stemonone were isolated by chromatographic procedures and identified by usual spectroscopic and spectrometric techniques. Clitoriacetal and 6-deoxyclitoriacetal were not found in all parts of the plant, such as leaves and petals, but in the roots they occur in higher concentration. The activity against brine shrimp revealed that the root extract (LD50 = 158 ppm) was the more active. PMID:27319136

  20. Evaluation of official instrumental methods for the determination of particulate matter contamination in large volume parenteral solutions.

    Science.gov (United States)

    Montanari, L; Pavanetto, F; Conti, B; Ponci, R; Grassi, M

    1986-11-01

    The distribution pattern of particle contamination in nine different types of LV parenteral solutions and the possibility of correlating the counts made with two official instruments (Coulter Counter and HIAC) were studied. Two hundred containers of LV parenteral solutions (corresponding to 40 batches) produced in Italy, were sampled. Each bottle was submitted to HIAC and Coulter Counter countings, for particle sizes ranging between 2 and 25 micron. For about 50% of the products, the two straight lines that represent the distribution of particle contamination obtained with the two methods did not cross-over within the studied size range, the Coulter Counter counts always proving higher than the HIAC ones. In the other cases, the cross-over point of the two lines occurred at varying size levels. Statistical analysis of the results pointed to a relationship between the contamination values obtained with the two counting methods for sizes ranging between 2 and 5 micron, but there was no correlation for sizes equal to, or higher than, 10 micron. From the maximum contamination levels established by the BP and the FU IX for the HIAC method, the corresponding values were calculated for the Coulter Counter method. Similarly the values were calculated the HIAC method based on the maximum values set for the Coulter Counter. PMID:2879006

  1. Determination of renal clearances of amylase/creatinine with chromogenic and enzymatic methods.

    Science.gov (United States)

    Hohenwallner, W; Wimmer, E; Sommer, R

    1979-12-01

    Urinary amylase was estimated by chromogenic (amylochrome Roche) as well as enzymatic methods (SKI and Beckman: substrate starch and substrate maltotetraose respectively). Random and timed urines (24 hour collections) were analysed. Clearances of amylase gave different results dependent upon the amylase-test used and the glomerular filtration rate. Correlation between chromogenic and enzymatic methods (starch as substrate) was poor. The ratio of amylase and creatinine clearance was used to test different methods. Reference values for this ratio for the amylochrome method (N = 106) were 2.85 +/- 0.99% and for the Beckman-DS method (N = 60) 2.82 +/- 0.87%.

  2. 反相高效液相色谱紫外法检测人血清尿酸%Determination of uric acid in human serum by reverse-phase high-performance liquid chromatography using ultraviolet detection

    Institute of Scientific and Technical Information of China (English)

    江雅平; 刘欧; 徐国宾; 陈文祥; 张传宝; 张江涛

    2012-01-01

    with the mean values of the participating laboratories. The correlation coefficients between reverse-phase HPLC-UV and 5 routine assay systems, i. e. , Hitachi, Beckman Coulter, Roche, Dade and Vitros were 0. 998 9, 0. 996 5 , 0. 999 2, 0. 999 2 and 0.998 7 respectively. The deviations between of reverse-phase HPLC-UV and 5 routine assay systems were all less than biological variation (5% ). Conclusion The reverse-phase HPLC-UV developed in this study displayed as a simple, specific, rapid, precise and accurate method, and its results showed close correlation and consistency with routine traceable systems, so it can be recommended as a candidate reference method for determination of uric acid in human serum.

  3. Differential microfluidic sensor on printed circuit board for biological cells analysis.

    Science.gov (United States)

    Shi, Dongyuan; Guo, Jinhong; Chen, Liang; Xia, Chuncheng; Yu, Zhefeng; Ai, Ye; Li, Chang Ming; Kang, Yuejun; Wang, Zhiming

    2015-08-01

    Coulter principal based resistive pulse sensor has been demonstrated as an important platform in biological cell detection and enumeration since several decades ago. Recently, the miniaturized micro-Coulter counter has attracted much attention due to its advantages in point of care diagnostics for on chip detection and enumeration of rare cells, such as circulating tumor cells. In this paper, we present a microfluidic cytometer with differential amplifier based on Coulter principle on a SU-8 coated printed circuit board substrate. The electrical current changes induced by the blockage of the microparticles in the sensing aperture are calibrated by polystyrene particles of standard size. Finally, HeLa cells are used to evaluate the performance of the proposed device for enumeration of biological samples. The proposed cytometer is built upon the cheap and widely available printed circuit board substrate and shows its great potential as personalized healthcare monitor.

  4. Symptoms and Pathogens of Nine Diseases of Ginseng%人参9种病害的症状和病原物

    Institute of Scientific and Technical Information of China (English)

    白容霖

    2002-01-01

    报道了人参的5种新病害:人参褐斑病(Acremoniella cucurbitae Schulz.et Sacc.)、人参黑腐病(Doratomyces stemonitis (Pers.et Fr.) Morton & Smith)、人参白粉病(Erysiphe panacis Bai et Wang)、人参褐腐病(Monilia cinerea Bon)、人参线虫病(Meloidogyne hapla Chitwood)和国内新记录的3种人参病害:人参灰霉病(Botrytis cinerea Pers.)、人参黑干腐病(Phoma panacicola Nakata et Takimoto)、人参粘菌病(Stemonitis herbatica Peck.).订正了吉林省人参细菌性软腐病的病原物,即为胡萝卜软腐欧文氏菌胡萝卜软腐亚种(Erwinia carotovora subsp. carotovora Dye)、胡萝卜欧文氏菌黑胫亚种(E.carotovora subsp. atroseptica Dye)、石竹假单胞菌(Pseudomonas caryophyll (Burkholder) Starr & Burkholder).

  5. Particles in small volume injections.

    Science.gov (United States)

    Taylor, S A; Spence, J

    1983-12-01

    The level of particulate contamination in small volume injections has been examined using the light blockage (HIAC) and electrical sensing zone (Coulter counter) techniques, the HIAC system being found to be the more suitable. Particle counts on the same batch of injection showed a large and variable difference between the HIAC and the Coulter counter results, especially below 5 micron. None of the injections examined complied with the British Pharmacopoeia limits for particulates in large volume parenterals, suggesting the unsuitability of the limits for small volume parenterals. PMID:6141237

  6. Determination of spectrophotometric absorptivity by analytical ultracentrifugation

    Directory of Open Access Journals (Sweden)

    M Senthilraja

    2011-01-01

    Full Text Available Rapid determination of the absorptivity for a recombinant IgG monoclonal antibody using the Beckman equipped with both Raleigh interference and UV absorbance optical systems. The analytical ultracentrifuge data for determining spectrophotometric absorptivities is compared to experimental data from quantitative amino acid analysis and an enzymatic digestion method.

  7. Course Management Systems and Blended Learning: An Innovative Learning Approach

    Science.gov (United States)

    Chou, Amy Y.; Chou, David C.

    2011-01-01

    This article utilizes Rogers' innovation-decision process model (2003) and Beckman and Berry's innovation process model (2007) to create an innovative learning map that illustrates three learning methods (i.e., face-to-face learning, online learning, and blended learning) in two types of innovation (i.e., incremental innovation and radical…

  8. Analysis of fractal magnitude of soil particles in loamy Chao soils in North China Plain%华北冲积平原壤质潮土的土壤粒径分形空间尺度分析

    Institute of Scientific and Technical Information of China (English)

    李晓鹏; 刘建立; 张佳宝; 王伟鹏; 辛文文

    2014-01-01

    It is widely accepted that soils have fractal characteristics in certain spatial scales. However, the exact magnitude of this spatial self-similarity is often neglected when doing fractal related researches. In this study, 43 sets of soil particle size distribution data of the Chao soil in North China Alluvial Plain were collected, and their particle size distributions with 93 levels were determined by laser diffraction analysis method (Beckman Coulter LS 13-320).The spatial magnitude orders of soils can be taken as fractal objectives and their affecting factors were analyzed based on the particle size distribution data. For determining the fractal magnitude of the soils, we used a typical scale method:1) calculate the differential fractal dimensions in all the 93 particle size levels (Di, i=1, 2,…, 93) according to the definition fractal; 2) choose a typical scale which is supposed to follow self-similarity (however, the measured Di values may still fluctuate in a certain range); 3) suppose Di in the typical scale is normally distributed, and calculate the corresponding distribution characteristics; 4) use the distribution characteristics to find out the lower and upper limits of Di which follows the same normal distribution in the whole range from the lower to upper limit (in the study, 0.5%and 99.5%of the accumulation curve were taken as the lower and upper limits, respectively);5) fit Di in the entire fractal magnitude, and the slope is supposed to be the fractal dimension of the soil.The results showed that the Chao soils in the study area mostly had good gradations, and they only had rigorous fractal structure at small to medium particle sizes (large clay to fine silt), but didn’t show spatial self-similarity in coarse sand scale. Soils with more clay particles usually had larger fractal dimensions, and the extents of fractal reduced with the increasing of clay fraction. Soils with good graduations usually had wider fractal extents. The fractal dimension

  9. 不同地区招飞体检学生静脉血细胞参数对比分析%Comparison on the venous blood cells parameters of the candidates in physical examination for pilot recruitment in different areas

    Institute of Scientific and Technical Information of China (English)

    厉淑红; 郑军; 周金立; 马新伟

    2015-01-01

    Objective To analyze the difference and variation rule of the parameters of venous blood cells in the pilot candidates from different areas,and provide a reference for the revision of physical examination standard and physical evaluation.Methods The candidates were divided into seven groups:East China,South China,Central China,North China,Northwest China,Southwest China,and Northeast China,according to the areas they came from,and 224 candidates were randomly selected from each group.2 ml morning fasting venous blood was collected into a dipotassium ethylene tetraacetate anticoagulation tube.As the sample was mixed homogenously,parameters of blood cells were determined by Beckman Coulter system 2 Automated Hematology Analyzer and the results were shown in the form of mean ± standard deviation (x±s).The statistical analysis was performed by using one-way analysis of variance (ANOVA),and it took P<0.05 as the statistical significance of difference.Results Comparing the blood cell parameters of the candidates from different areas,WBC,neutrophilic granulocyte,lymphocyte,and monocyte showed no statistical significance (P > 0.05);eosinophilic granulocyte and basophilic granulocyte showed statistical significance (F=7.906,2.524,P<0.01);all parameters of red blood cell and platelet showed statistical significance (F=4.435-14.864,F=4.847-20.657,P<0.01).The percentage of abnormality was respectively 18,11% in RBC,43.56% in Hb,14.16% in hemoglobin,17.54% in lymphocyte,9.06% in neutrophilic granulocyte,and 1.40% in platelet.Conclusions There are regional differences in venous blood cell parameters among the pilot candidates from different areas.It is necessary to improve the items of blood cell test and the evaluation standard considering the area difference in order to obtain more accurate physical evaluation and the references for the tracking survey of the blood cell parameters in pilots.%目的 分析我国不同地区招飞体检学生静脉血细

  10. Pikantsed pajatused / Aita Kivi

    Index Scriptorium Estoniae

    Kivi, Aita, 1954-

    2000-01-01

    Sisu : Louis Paul Boon. Menuett; Herbjırg Wassmo. Õnne poeg; Jean Stone. Sünnipäevatüdrukud; Jo︠l Schmidt. Constantinus Suure mälestused; Mari Vallisoo. Ainsuse olevik; Catherine Coulter. Märklaud; Ross Macdonald. Mees mulla all

  11. Proxies and measurement techinques for mineral dust in antarctic ice cores

    DEFF Research Database (Denmark)

    Ruth..[], Urs; Bigler, Matthias

    2008-01-01

    To improve quantitative interpretation of ice core aeolian dust records, a systematic methodological comparison was made. This involved methods for water-insoluble particle counting (Coulter counter and laser-sensing particle detector), soluble ion analysis (ion chromatography and continuous flow...

  12. Application of particle size distributions to total particulate stack samples to estimate PM2.5 and PM10 emission factors for agricultural sources

    Science.gov (United States)

    Particle size distributions (PSD) have long been used to more accurately estimate the PM10 fraction of total particulate matter (PM) stack samples taken from agricultural sources. These PSD analyses were typically conducted using a Coulter Counter with 50 micrometer aperture tube. With recent increa...

  13. Optimization, Application, and Interpretation of Lactate Dehydrogenase Measurements in Microwell Determination of Cell Number and Toxicity

    NARCIS (Netherlands)

    Wolterbeek, H.T.; Van der Meer, A.J.G.M.

    2005-01-01

    The lactate dehydrogenase (LDH) assay was addressed for its sensitivity, disturbances by foaming, and cell number and size. Cells were from a U-251 MG grade IV human glioblastoma brain tumor cell line used in 100-µl well volumes. Cells were counted by microscopy and Coulter counting; assays were LDH

  14. 2x Ben Affleck / Laurence Lumiere

    Index Scriptorium Estoniae

    Lumiere, Laurence

    2007-01-01

    Põnevusfilm "Hollywoodland" : režissöör Allen Coulter : Ameerika Ühendriigid 2006 ja põnevuskomöödia "Äss mättasse" ("Smokin' Aces") : režissöör Joe Carnahan : Suurbritannia - Prantsusmaa - Ameerika Ühendriigid 2007. Mõlemas mängib Ben Affleck

  15. Effects of injection systems and plastic mulches on distribution and emissions of cis- and trans-1,3-dichloropropene and chloropicrin.

    Science.gov (United States)

    Ou, Li-Tse; Thomas, John E; Allen, L Hartwell; Vu, Joseph C; Dickson, Donald W

    2007-08-01

    The concentration and distribution of a soil fumigant in the subsurface of field plots are two key factors in the determination of the fumigant efficacy. Subsurface concentrations of the biologically active compounds cis- and trans-1,3-dichloropropene (1,3-D) and chloropicrin (CP) were determined in soil at two adjacent injection traces and midpoint between the two traces in plastic-covered field beds after injection of the fumigant Telone C35 by conventional chisels or by a coulter rig (Avenger coulters). Two of the four beds were covered with metallic polyethylene film (MPE) and the remaining two were covered with virtually impermeable film (VIF). Three hours after chisel injection, concentrations of the three compounds at the two adjacent injection traces in the two beds were highly variable. Large concentrations of the compounds were detected at the side traces, whereas the compounds were not detected at the middle traces (bed centers) in the two chisel-injected beds covered with MPE or VIF. Initial concentrations of the three compounds at the two adjacent injection traces in the two Avenger-coulter-injected beds were more uniform than in the chisel-injected beds but still variable. Also, the three compounds had diffused horizontally to midpoint between the two injection traces in all four beds, generally in smaller concentrations. Concentrations of the three compounds in the shallow subsurface soil layer (0.5-30 cm) in the beds became progressively more uniform and then declined slowly. Volatilization losses from the two Avenger-coulter-injected beds and the chisel-injected bed covered with VIF were low. Combination of Avenger coulter injection and VIF provided better retention of the compounds in this soil layer and, thus, likely would provide better fumigant efficacy and crop yield.

  16. Diversity of plasmodial slime molds (myxomycetes in coastal, mountain, and community forests of Puerto Galera, Oriental Mindoro, the Philippines

    Directory of Open Access Journals (Sweden)

    Nikki Heherson A. Dagamac

    2015-12-01

    Full Text Available No profiling of diversity of myxomycetes has ever been conducted in one of the biodiversity hotspot areas in the Philippine archipelago, and this necessitates a swift survey of myxomycetes in Puerto Galera, Oriental Mindoro. An assessment of diversity of myxomycetes collected from seven collecting points of three different forest types in the study area showed a total of 926 records of myxomycetes. Of which, 42 morphospecies belonging to 16 genera are reported in this study. Species richness of myxomycetes was higher in collecting points that were found in inland lowland mountain forests, but the most taxonomically diverse species was found in coastal forests. Myxomycete species, namely, Arcyria cinerea, Diderma hemisphaericum, Physarum echinosporum, Lamproderma scintillans, and Stemonitis fusca, were found in all the collecting points. Manmade disturbances and forest structure may affect the occurrence of myxomycetes.

  17. Historical, ecological, and governance aspects of intensive forest biomass harvesting in Denmark

    DEFF Research Database (Denmark)

    Stupak, Inge; Raulund-Rasmussen, Karsten

    2016-01-01

    Intensive forest harvesting has increased in Fennoscandia over the last few decades. Similar developments may follow throughout Europe as renewable energy replaces fossil fuels. The international literature suggests that intensive harvesting could increase ecological risks to yield, carbon stores......, soil fertility, and biodiversity, but geographically specific knowledge is sparse in many countries, and results do not extend beyond 5–30 years after harvesting. We use Denmark as a case for discussing future directions. Forest history is described, and research on ecological effects...... and their inclusion in governance is reviewed. Denmark was almost completely deforested by the early 1800s, but centuries of afforestation have resulted in a current forest cover of 14.3%. Research commonly uses stem-only harvesting as a reference against which to compare intensive harvesting impacts, but pristine...

  18. Reconciling Harvest Intensity and Plant Diversity in Boreal Ecosystems: Does Intensification Influence Understory Plant Diversity?

    Science.gov (United States)

    Kershaw, H Maureen; Morris, Dave M; Fleming, Robert L; Luckai, Nancy J

    2015-11-01

    Overall demand for forest products in the boreal forest is increasing to supply growing bio-energy demands in addition to traditional forest products. As a result, there is a need to refine current forest policies to reconcile production and ecosystem function within the context of ecologically sustainable management. This study assessed understory plants' richness, evenness, and diversity in six harvested boreal black spruce-dominated stands situated on loam, sand, and peat site types 15 years after the application of four harvest treatments of increasing biomass removals. Treatments included uncut, stem-only harvest, full-tree harvest, and full-tree harvest + blading of O horizon. Following canopy removal, species richness and diversity (Shannon's and Simpson's indices) increased on all soil types. The more than doubling of slash loading on the stem-only treatment plots compared to the full-tree plots led to significantly lower species diversity on loam sites; however, the reverse was observed on peat sites where the slash provided warmer, drier microsites facilitating the establishment of a broader array of species. Preexisting ericaceous shrub and sphagnum components continued to dominate on the peat sites. Compositional shifts were most evident for the full-tree + bladed treatment on all soil types, with increases in herbaceous cover including ruderal species. The results suggest that the intensification of harvesting on plant diversity varies with soil type, and these differential results should be considered in the refinement of forest biomass-harvesting guidelines to ensure ecological sustainability and biodiversity conservation over a broad suite of soil types. PMID:26092048

  19. N cycling and the composition of terpenes and tannins in boreal forest soils: Effects of logging residues

    Science.gov (United States)

    Smolander, Aino; Kitunen, Veikko; Kukkola, Mikko; Tamminen, Pekka

    2014-05-01

    There is increasing evidence available that certain terpenes and tannins may mediate substantial changes in nitrogen cycling processes in boreal forest soils. Terpenes and tannins are two important groups of plant secondary metabolites: Terpenes are hydrocarbons having different number of isoprene-derived units and tannins are complex polyphenolic compounds able to interact with proteins. Logging residues, consisting of fresh tree tops and branches with needles contain large amounts of terpenes and tannins. Currently there is increasing demand for forest biomass for bioenergy production. Therefore, harvesting of logging residues has become more common from both clear-cutting and thinning stands, instead of conventional stem-only harvest where logging residues are retained on the site. Our aim was to determine how logging residues affect soil N cycling processes in Scots pine and Norway spruce thinning stands in long-term, and how these processes are related to the composition of terpenes and tannins in the soil. Samples were taken from the humus layer of pine and spruce experiments which had been thinned 4-to-19 years before; in the thinning different amounts of logging residues had been distributed on the plots. Logging residues had only little effect on soil microbial biomass N or C. However, in several sites logging residues increased the rate of net N mineralization and the ratios net N mineralization/ C mineralization and net N mineralization/microbial biomass N, and these positive effects were very long-lasting. Logging residues also changed the composition of different terpenes and condensed tannins in soil. In general, with regard to the processes and ratios indicating N availability, stem-only harvest seems to be more favorable than whole-tree harvest. The results from long-term field experiments will be discussed in relation to the effects of different terpenes and tannins, observed in short-term laboratory experiments, on N cycling processes.

  20. Effects of harvesting and soil disturbance on soil CO2 efflux from a jack pine forest

    International Nuclear Information System (INIS)

    This study measured the effects of organic matter removal and soil compaction on soil surface carbon dioxide (CO2) efflux (F) from a coarse-textured jack pine forest. F represents the major source of carbon dioxide loss from forests and is highly responsive to both soil temperature and soil moisture in jack pine stands. An analytical framework was developed for deriving biologically based empirical response functions to assess the importance of factors controlling F treatment responses and to model treatment effects. Treatments included stem-only harvest (OM0C0); full-tree harvest (OM1C0); full-tree harvest with surface soil removal (OM2C0); full-tree harvest with surface soil removal and soil compaction (OM2C2); and uncut forest. Three to 5 years after harvest, growing-season F was similar following stem-only and full-tree logging compared with uncut forest. It was much greater than in harvested areas with surface soil removal. Mean F and calculated F at 10 degrees C under non-limiting soil moisture conditions (F10) were greatest in treatments with intact organic surfaces. F10 showed a strong linear relationship with detrital production in harvested plots, with total near-surface carbon in all plots, and was positively correlated with ground cover. F increased exponentially with soil temperature and responded parabolically to relative soil water content. F was often low in May in the uncut forest because of cold soils, but then attained rates equivalent to those of the OM0C0 and OM1C0. The trends reflect treatment effects on substrate availability, rooting density and soil temperature and moisture. In general, F was influenced more by post harvest removal of surface organic horizons rather than by clear-cut harvesting. 71 refs., 4 tabs., 5 figs

  1. Evaluation of four gentamicin and tobramycin assay procedures for clinical laboratories.

    OpenAIRE

    Witebsky, F G; Sliva, C A; Selepak, S T; Ruddel, M E; MacLowry, J D; Johnson, E E; Elin, R J

    1983-01-01

    Accuracy, precision, and clinical laboratory utility of the TDX (Abbott Laboratories), Auto-ICS (Beckman Instruments, Inc.), COBAS-Bio (Roche Analytical Instruments, Inc.) with reagent kits (Syva), and EMIT (Syva) for gentamicin and tobramycin serum assay were assessed. TDX, COBAS-Bio, and EMIT analytical systems showed a proportional bias of less than 10% for recovery studies and a coefficient of variation less than 5% for within-run precision. The results of the recovery studies with the Au...

  2. High molecule compound synthesis from carbon dioxide; Nisanka tanso karano kobunshi gosei

    Energy Technology Data Exchange (ETDEWEB)

    Mishima, K. [Fukuoka Univ., Fukuoka (Japan)

    2000-09-05

    Recently, synthesis of organic substances and high molecule compounds using carbon dioxide of liquid or supercritical state as raw material is paid attention so as the influential means to realize the green chemistry. A zinc based catalyst which could synthesize the activated copolymer from oxidized cyclohexane and carbon dioxide was developed by Coates, Beckman et al. The high-activated fluoridation zinc based catalyst which dissolved the high-pressure carbon dioxide was developed by Beckman, etc. The possibility of the manufacturing of the high molecule copolymer which is made from carbon dioxide and oxidized cyclohexane as a raw material was shown by an appearance of this catalyst. In addition, a synthesizing method of lactone from carbon dioxide and olefinic hydrocarbon is being examined by Beckman. If this method is used practically, the manufacturing of a gentle biodegradable polymer in the environment which is made from carbon dioxide as a raw material becomes also possible. However, adequate catalysts are necessary so that this reaction may progress. (NEDO)

  3. New micromethod for measuring cholesterol in plasma lipoprotein fractions.

    Science.gov (United States)

    Bronzert, T J; Brewer, H B

    1977-11-01

    A method is described for the reliable, fast, and relatively inexpensive fractionation of plasma lipoproteins and quantitation of their cholesterol content. This procedure requires 350 microliter of plasma and can be completed within 3 h. Plasma lipoproteins (175 microliter of plasma) were prestained with Fat Red 7B and centrifuged (Beckman Airfuge) at plasma density (d = 1.006 kg/liter) and at a solvent density of 1.060 kg/liter, adjusted by adding solid KBr. Prestained centrifuged samples demonstrated the characteristic elevation of chylomicrons in phenotypes I and V, low-density lipoproteins of phenotype II, very-low-density lipoproteins in phenotype IV and V, and continuum of pink color throughout the centrifuge tube, diagnostic of the floating beta lipoprotein of type III. Centrifuged samples were separated into top and bottom fractions by aspiration. Cholesterol was quantitated with an enzymic oxygen-electrode analyzer (Beckman Cholesterol Analyzer). Correlation coefficients between cholesterol values for plasma from normal hyperlipidemic individuals obtained with the Beckman Analyzer vs. the Technicon AutoAnalyzer II and SMAC systems were 0.977 and 0.973, respectively.

  4. 长时间高原训练对优秀赛艇运动员免疫机能及血浆瘦素的影响%Effect of Long-term Altitude Training on the Immunity and Plasma Leptin of Elite Rowers

    Institute of Scientific and Technical Information of China (English)

    高欢; 高炳宏; 冯连世; 孟志军

    2011-01-01

    二者之间的关系.方法:20名优秀男子赛艇运动员进行持续60天的高原训练(海拔2200m),训练分为两个大的训练阶段,7天为1个训练周,每个训练周包含2个小周期.内容以中低强度有氧耐力训练为主.上高原后第1、4、10、25、38、60天,即每个训练小周期、训练周或大训练阶段调整结束后次日清晨,空腹采集前臂静脉血.酶联免疫吸附法测定血清瘦素,化学发光仪测定血清皮质醇,以Beckman COULTER ACT10血球仪测定白细胞及其分类计数,以Binding Site AD200光散射光度计测定免疫球蛋白.结果:(1)与上高原前的(6.55±1.31)×109/L比较,WBC计数在上高原后1天[(5.62±2.26)×109/L]下降14.2%,高原第10天为(6.51±0.97)×109/L,回升至接近高原前水平;高原第25天为(5.24±0.82)×109/L,与高原前、高原第10天比较显著下降(P<0.05). (2)LY%与WBC计数变化趋势近似,高原第25天[(29.37±5.88)]%大幅下降,与高原前的(40.43±6.64)%和高原第10天的(36.62±6.94)%比较有显著性差异(P<0.05);高原训练第38天、第60天LY%分别为(32.49±4.92)%、(28.18±3.71)%,均显著低于高原前.(3)上高原1天后,血清IgG[(12.11±2.18)g/L]显著升高(P<0.05);之后,IgG无明显变化;血清IgM和IgA高原训练阶段与高原前比较均无显著改变. (4)整个高原训练过程中,血清LP均值无显著性改变(变化范围6.12~6.56 ng/ml).结论:高原训练第25天左右,免疫机能可能下降;经10天调整训练后略有恢复,至上高原后第60天仍低于高原前.长时间高原训练过程中,血清LP与免疫机能之间的变化关系仍需研究.

  5. On the Calibration of a Size-Structured Population Model from Experimental Data

    CERN Document Server

    Jauffret, Marie Doumic; Zubelli, Jorge P

    2009-01-01

    The aim of this work is twofold. First, we survey the techniques developed in (Perthame, Zubelli, 2007) and (Doumic, Perthame, Zubelli, 2008) to reconstruct the division (birth) rate from the cell volume distribution data in certain structured population models. Secondly, we implement such techniques on experimental cell volume distributions available in the literature so as to validate the theoretical and numerical results. As a proof of concept, we use the data reported in the classical work of Kubitschek [3] concerning Escherichia coli in vitro experiments measured by means of a Coulter transducer-multichannel analyzer system (Coulter Electronics, Inc., Hialeah, Fla, USA.) Despite the rather old measurement technology, the reconstructed division rates still display potentially useful biological features.

  6. Two-stage cell shrinkage and the OER for radiation-induced apoptosis of rat thymocytes

    International Nuclear Information System (INIS)

    Suspensions of rat thymocytes were given 0.09-100 Gy using 60Co γ-rays. The radiation-induced changes in the thermocytes were examined from minutes to hours post-irradiation using electron microscopy, agarose gel electrophoresis, staining and Coulter Counter sizing. Sizing by Coulter Counter showed, for the first time, that thermocytes which undergo apoptosis shrink in two distinct stages, first by a sudden decrease from an original volume of 99 μm3 to a volume of 76 μm3, followed by a gradual decrease to 57 μm3 over the space of a few hours. The oxygen enhancement ratio for apoptosis was measured to be about 3.5, similar to the value for reproductive death for many mammalian cells. (author)

  7. Lower Total Adipocyte Number but No Evidence for Small Adipocyte Depletion in Patients With Type 2 Diabetes

    OpenAIRE

    Pasarica, Magdalena; Xie, Hui; Hymel, David; Bray, George; Greenway, Frank; Ravussin, Eric; Smith, Steven R.

    2009-01-01

    OBJECTIVE We hypothesized that, compared with obese subjects, patients with type 2 diabetes have a lower total adipocyte number with fewer small adipocytes. RESEARCH DESIGN AND METHODS Abdominal subcutaneous adipose tissue was obtained from lean and obese subjects with or without type 2 diabetes matched for BMI. Adipocyte size was measured by osmium fixation and sizing/counting in a Coulter counter. Adipocyte size and number subdistributions (small, medium, large, and very large) were determi...

  8. A manual bead assay for the determination of absolute CD4+ and CD8+ lymphocyte counts in human immunodeficiency virus-infected individuals.

    OpenAIRE

    Carella, A V; Moss, M.W.; Provost, V; Quinn, T C

    1995-01-01

    CD4+ T lymphocytes are currently the most common surrogate marker indicating disease progression in individuals infected with human immunodeficiency virus (HIV). Since the cost of enumerating lymphocyte phenotypes is quite high, an inexpensive bead assay analyzed by light microscopy (cytosphere assay; Coulter Corporation, Hialeah, Fla.) was developed as an alternative method for counting CD4+ and CD8+ T lymphocytes. To evaluate the reliability of the cytosphere assay, heparinized blood was co...

  9. Biosensor Scheme for the Determination of Intracellular Pressure of Erythrocyte

    Directory of Open Access Journals (Sweden)

    Yu.S. Nagornov

    2016-03-01

    Full Text Available The paper presents a scheme of the biosensor for determining the intracellular pressure of erythrocytes. The possibility of measuring of the volume and area of the erythrocyte is provided in a biosensor to determine the value intracellular pressure. In MEMS this creates flow that enters into Coulter capacitive sensor through the rate control system and then in the system of signal transmitting. The definition of erythrocyte volume and calculation of intracellular pressure occur in the computer system.

  10. Miniaturization of powder dissolution measurement and estimation of particle size.

    Science.gov (United States)

    Avdeef, Alex; Tsinman, Konstantin; Tsinman, Oksana; Sun, Na; Voloboy, Dmytro

    2009-11-01

    The objective was to investigate the applicability and limitations of an approach for estimating particle size from powder dissolution measurement using as little as 50 microg of sample in 1 ml of buffer solutions. The powder dissolution profiles of five sparingly-soluble drugs (hydrochlorothiazide, phenazopyridine hydrochloride, 2-naphthoic acid, indomethacin, and dipyridamole) were evaluated with a novel biexponential spherical particle equation and also the Wang-Flanagan spherical particle non-sink equation. The results were compared to particle sizing based on measured specific surface area by the Brunauer-Emmett-Teller (BET) method, and also based on Coulter counting. With the exception of hydrochlorothiazide, the model compounds indicated some agglomeration in the dissolution media. The dry-state specific surface area was larger than expected from either the Coulter method or the powder-dissolution data, especially for phenazopyridine hydrochloride. The particle radii estimated by the powder dissolution method ranged from 10 to 68 microm, with equilibrium solubilities spanning from 5 microg/ml (dipyridamole) to 911 microg/ml (hydrochlorothiazide). Powder dissolution data collected with the miniaturized apparatus can be used to determine particle size, with estimated values agreeing reasonably with those measured by the Coulter counter method. PMID:19937817

  11. Solar energy in the United States

    International Nuclear Information System (INIS)

    Written by a group of five French experts who visited several research centres, innovating companies and solar power stations in the United States, this report first proposes an overview of solar energy in the United States, indicating and commenting the respective shares of different renewable energies in the production, focusing on the photovoltaic energy production and its RD sector. The second part presents industrial and research activities in the solar sector, and more specifically photovoltaic technologies (silicon and thin layer technology) and solar concentrators (thermal solar concentrators, photovoltaic concentrators). The last chapter presents the academic research activities in different universities (California Tech Beckman Institute, Stanford, National Renewable Energy Laboratory, Colorado School of Mines)

  12. Determination of average molecular weights on organic reactor coolants. II.-Freezing point depression method for diphenyl-ether solutions; Determinacion de masas moleculares medias en refrigerantes nucleares organicos. II.- Crioscopia de disoluciones en eter difenilico

    Energy Technology Data Exchange (ETDEWEB)

    Carreira, M.

    1965-07-01

    In order to reduce limitations of solubility, the cryoscopic method developed for benzene solutions of polyphenyl mixtures has been extended to diphenyl-ether solutions by introducing some modifications imposed by the physico-chemical properties of this solvent. The Nernsto theory of Beckman's method has been revised, taking into account the heat-transfer characteristics of the system, and the results of that analysis have been used to fix upon the design parameters of a cryoscopic apparatus for measurements on diphenyl-ether solutions. (Author) 9 refs.

  13. Observations on the morphology and chemical analysis of medullary granules in chinchilla hair. Research letters

    Energy Technology Data Exchange (ETDEWEB)

    Keogh, H.J. (South African Inst. for Medical Research, Johannesburg); Haylett, T. (Council for Scientific and Industrial Research, Pretoria (South Africa). National Chemical Research Lab.)

    1983-02-01

    The ultrastructure of the medullary granules of white and grey chinchilla hair was investigated by scanning electron microscopy and chemical analysis in an attempt to clarify their structure and function. Atomic absorption spectroscopy and amino acid analysis showed them to be composed of melanin. The sample preparation for scanning electron microscopy is discussed. The metal content was qualitatively established by X-ray fluorescence spectrometry and quantitatively determined on a Varian Techtron model AAs atomic absorption spectrophotometer. Amino acid analysis of the granule, was carried out on a Beckman 121 amino acid analyser. Information is provided on the amino acid composition of the medullary granules as well as its metal content.

  14. Observations on the morphology and chemical analysis of medullary granules in chinchilla hair

    International Nuclear Information System (INIS)

    The ultrastructure of the medullary granules of white and grey chinchilla hair was investigated by scanning electron microscopy and chemical analysis in an attempt to clarify their structure and function. Atomic absorption spectroscopy and amino acid analysis showed them to be composed of melanin. The sample preparation for scanning electron microscopy is discussed. The metal content was qualitatively established by X-ray fluorescence spectrometry and quantitatively determined on a Varian Techtron model AAs atomic absorption spectrophotometer. Amino acid analysis of the granule, was carried out on a Beckman 121 amino acid analyser. Information is provided on the amino acid composition of the medullary granules as well as its metal content

  15. A comparison of analytical methods for detection of [14C]trichloro acetic acid-derived radioactivity in needles and branches of spruce (Picea sp.)

    International Nuclear Information System (INIS)

    The branches (wood and needles) of spruces of varying age treated with [14C]trichloro acetic acid (3.7 GBq/mmol) were studied, using the following methods: Qualitative: - Conventional macroautoradiography with X-ray film and histological classification. Quantitative: - 14C combustion analysis with the sample oxidizer A 307 (Canberra/Packard) followed by measurement of radioactivity using the LS counter 6000 (Beckman Instrumentts); - digital autoradiography with the Digital Autoradiograph LB 286 (Berthold GmbH); -digital autoradiography with the Bio-imaging Analyzer BAS 2000 (Fuji Film Co.). (orig.)

  16. DIVERSITY AND DISTRIBUTION OF PLASMODIAL MYXOMYCETES (SLIME MOLDS FROM LA MESA ECOPARK, QUEZON CITY, PHILIPPINES

    Directory of Open Access Journals (Sweden)

    THOMAS EDISON E. DELA CRUZ

    2010-10-01

    Full Text Available Myxomycetes are ubiquitous in terrestrial forest ecosystems. Thus, this research study looks at the taxonomic diversity and distribution of plasmodial myxomycetes in La Mesa Ecopark in Quezon City, Philippines. A total of 240 moist chambers were prepared from four substrates (aerial and ground leaf litter, twigs and barks collected within this ecopark. Following incubation of moist chambers for eight weeks, a total of 28 species belonging to 10 genera were collected and identified: Arcyria (3, Diderma (2, Didymium (5, Lamproderma (2, Perichaena (3, Physarum (8, Macbrideola (1, Metatrichia (1, Trichia (1 and Stemonitis (2. Highest myxomycete yield (85% was observed in aerial leaf litter. In terms of taxonomic diversity, highest diversity was observed in bark microhabitats, although the lowest number of species was recorded in it. Assessment of their abundance and distribution showed similarities in species composition between aerial and ground leaf litter. This research study is the first report of plasmodial myxomycetes in La Mesa Ecopark in Quezon City, Philippines.

  17. Coprophilous fungi of the horse.

    Science.gov (United States)

    Pointelli, E; Santa-maria, M A; Caretta, G

    1981-05-01

    A total of 1267 microfungi, including 35 Myxomycetes, were recorded from the fecal samples of the 60 horses; of these 395 were found on 20 saddle-horse feces, 363 on 20 race-horses and 509 on 20 working horses. Eighty two species representing 53 genera were recorded; of these 7 were Zygomycetes, 18 Ascomycetes, 1 Basidiomycetes and 25 Fungi Imperfecti: 2 Myxomycetes. Common coprophilous fungi are in decreasing order Pilobolus kleinii, Saccobolus depauperatus, Mucor hiemalis, Lasiobolus ciliatus, Podospora curvula, Petriella guttulata, M. circinelloides, Coprinus radiatus, Dictyostelium mucoroides, Sordaria fimicola, C. miser, C. stercorariusm, Acremonium sp., Coprotus granuliformis, Graphium putredinis, Iodophanus carneus, Chaetomium murorum, Podospora communis, P. inaequalis, P. setosa, Saccobolus versicolor and Cladosporium cucumerinum. Species of Myrothecium verrucaria, Actinomucor elegans, Kernia nitida, Spiculostilbella dendritica and Mucor parvispora were found exclusively in working-horses feces. Badhamia sp., Anixiopsis stercoraria, Echinobotryum state of D. stemonitis, Geotrichum candidum and Oidiodendron sp. were found only in saddle-horses feces. Chlamidomyces palmarum, Philocopra sp. were found exclusively in race-horses feces. Notes on infrequent or interesting fungi include Thamnostylum piriforme, Phialocephala dimorphospora, Rhopalomyces elegans and Spiculostilbella dendritica. PMID:7242651

  18. Crystallization Thermodynamic and Kinetic Behaviors of Vitamin C in Batch Crystallizer

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The bench-scale cooling crystallization for ternary solution of L-ascorbic acid (Vitamin C) was studied. The solid-liquid phase diagram of Vitamin C-water-ethanol system was obtained on the basis of differential scanning calorimeter (DSC) curves. The heat of crystallization of Vitamin C was calculated with the aid of quantitative analysis. According to the population balance equation under unsteady state, the rates of nucleation and growth were determined. The parameters of crystallization kinetics equations were estimated by regression of experimental data. Crystal morphology and size were determined with x-ray diffraction and TA Ⅱ Coulter Counter.

  19. Glaciological and chemical studies on ice cores from Hans Tausen ice cap, Greenland

    DEFF Research Database (Denmark)

    Clausen, H.B.; Stampe, Mia; Hammer, C.U.;

    2001-01-01

    The paper presents studies of various chemical and isotopical parameters from ice cores drilled in the northernmost located ice cap, Hans Tausen Iskappe, Pearyland, Greenland (HT). The 346 m main core (MC95) was drilled to bedrock in 1995 as well as a 35 m shallow core (SC95). A 60 m shallow core...... are selected for an analysis of dust and water soluble chemical components, including F-, CH3SO2-, Cl-, NO3-, SO42-, Na+, NH4+, K+, Mg2+ and Ca2+. Coulter counter technique was used for the dust measurements and the chemical analysis were carried out by ion chromatography....

  20. Geochemical variability of copper and iron in Oman margin sediments

    Digital Repository Service at National Institute of Oceanography (India)

    Alagarsamy, R.

    in the laboratory. 2.1. Analytical Methods Sediment grain size distribution in each section of cores collected below the OMZ was determined with a Coulter Counter (Model LS 130). Water content in each section of cores collected below the OMZ was determined...-rate estimates suggested that some bioturbation occurring below the mixed-layer depths. Mean Peclet numbers (i.e., [mixed layer depth]x[sedimentation rate]/[D b ]) for the mixed layer were ≤0.4 at five of six stations in the Oman Margin; at 1000 m, a...

  1. Cell volume increase in murine MC3T3-E1 pre-osteoblasts attaching onto biocompatible Tantalum observed by magnetic AC mode Atomic Force Microscopy

    Directory of Open Access Journals (Sweden)

    Klembt Andersen L.

    2005-12-01

    Full Text Available Magnetic AC mode (MACmode atomic force microscopy (AFM was used to study murine (mouse MC3T3-E1 preosteoblastic cells attached to biocompatible tantalum substrates. Cell volumes of attached cells derived from AFM images were compared to volumes of detached cells in suspension measured by the Coulter sizing technique. An increase of similar 50 % in cell volume was observed when the cells attached to planar tantalum substrates and developed a flattened structure including lamellipodia. We address thoroughly the issues general to the AFM determination of absolute cell volumes, and compare our magnetic AC mode AFM measurements to hitherto reported cell volume determinations by contact mode AFM.

  2. APSAS; an Automated Particle Size Analysis System

    Science.gov (United States)

    Poppe, Lawrence J.; Eliason, A.H.; Fredericks, J.J.

    1985-01-01

    The Automated Particle Size Analysis System integrates a settling tube and an electroresistance multichannel particle-size analyzer (Coulter Counter) with a Pro-Comp/gg microcomputer and a Hewlett Packard 2100 MX(HP 2100 MX) minicomputer. This system and its associated software digitize the raw sediment grain-size data, combine the coarse- and fine-fraction data into complete grain-size distributions, perform method of moments and inclusive graphics statistics, verbally classify the sediment, generate histogram and cumulative frequency plots, and transfer the results into a data-retrieval system. This system saves time and labor and affords greater reliability, resolution, and reproducibility than conventional methods do.

  3. Integrin β1, Osmosensing, and Chemoresistance in Mouse Ehrlich Carcinoma Cells

    DEFF Research Database (Denmark)

    Sørensen, Belinda Halling; Rasmussen, Line Jee Hartmann; Broberg, Bjørn Sindballe;

    2015-01-01

    RNA was used to silence integrin β1 expression. Regulatory volume decrease (RVD) after cell swelling was studied with calcein-fluorescence-self-quenching and Coulter counter analysis. Taurine efflux was estimated with tracer technique. Caspase assay was used to determine apoptosis. RESULTS: We show...... that adherent cells have stronger fibronectin binding and a significantly increased expression of integrin α5, αv, and β1 at mRNA and protein level, compared to non-adherent cells. Knockdown of integrin β1 reduced RVD of the adherent but not of the non-adherent cells. Efflux of taurine was unaffected...

  4. Fröplacering och vertikal rörelse för en fjädrande såbill på Väderstads rapidsåmaskin

    OpenAIRE

    Karlsson, Johan

    2001-01-01

    For good crop emergence, the seed should be placed at a depth where good conditions concerning oxygen, temperature and water are provided. To place the seed in the soil, some type of see d coulter is used. Väderstad-Verkens Rapid is a seed drill that places the seed at a certain depth in relation to a wheel running on the ground. This can lead to a to shallow seed placement. On fields with clayey soils and spring sown cereals a to shallow seed placement can lead to poor emergence, especially ...

  5. ANALYSIS OF OPENERS OF SEEDERS IN RESOURCE SAVING TECHNOLOGIES OF GRAIN PLANTING

    Directory of Open Access Journals (Sweden)

    Priporov E. V.

    2015-05-01

    Full Text Available When sowing grain we apply resource saving technologies-technologies such as «no till» and a technology called «mini-till». «No till» involves planting crops on untreated surfaces. "Mini-till" is designed for the planting with minimum tillage. During the movement of the sowing unit on these agricultural backgrounds we can see working bodies tipping out. Stabilization of the drilling depth is ensured in two ways - by moving part of the weight of the planter on the opener or the installation of ballast weights. Openers of grain drills for resource saving technologies for grain crops can be divided into three groups – foot, disc and anchor. Disc coulter has varieties – single disc, double disc and montesquiu coulter. To analyze the cost of power for the operation of the opener, the following indicators – the minimum engine power of the tractor on the coulter and engine power of tractors per unit of working width. The calculation of the indicators was conducted using data the technical specifications of the planter. Snow coulter drill AGRATOR4800 provides a wide spread planting strip of 10-15cm. The minimum power of the engine of the tractor on the opener is 7.5 HP minimum power of tractor engine per unit of working width 25 HP/m. Double disc opener planter Great Plains has a minimum engine power of the tractor on the opener 2.7 HP and per unit of working width 26.0-28.44 HP/m. Planter equipped with disc knife for strip tillage. The planter Condor 1201 is provided with a chisel opener «ConTeC». The engine power of the tractor on the opener of the planter is 4.58 HP, the engine power of the tractor unit working width constitute l8.33 HP/m. This grain seeder has reduced power losses and provides sowing with a «no till» technology

  6. The challenges of testing metal and metal oxide nanoparticles in algal bioassays: titanium dioxide and gold nanoparticles as case studies

    DEFF Research Database (Denmark)

    Hartmann, Nanna Isabella Bloch; Engelbrekt, Christian; Zhang, Jingdong;

    2013-01-01

    in standardised algal growth inhibition tests are highlighted with specific focus on biomass quantification methods. This is illustrated through tests with TiO(2) and Au nanoparticles, for which cell-nanoparticle interactions and behavior was studied during incubation. Au NP coating layers changed over time...... and TiO(2) nanoparticle aggregation/agglomeration increased as a function of concentration. Three biomass surrogate measuring techniques were evaluated (coulter counting, cell counting in haemocytometer, and fluorescence of pigment extracts) and out of these the fluorometric methods was found to be most...

  7. Interstitial diffuse radiance spectroscopy of gold nanocages and nanorods in bulk muscle tissues

    OpenAIRE

    Grabtchak S; Montgomery LG; Pang B; Wang Y; Zhang C; Li Z; Xia Y; Whelan WM

    2015-01-01

    Serge Grabtchak,1,2 Logan G Montgomery,1 Bo Pang,3,4 Yi Wang,4,5 Chao Zhang,6,7 Zhiyuan Li,6,7 Younan Xia,4,8 William M Whelan1,91Department of Physics, University of Prince Edward Island, Charlottetown, PEI, Canada; 2Departments of Electrical and Computer Engineering, and Physics, Dalhousie University, Halifax, Canada; 3Department of Biomedical Engineering, Peking University, Beijing, People’s Republic of China; 4The Wallace H Coulter Department of Biomedical Engineering, Georgia I...

  8. Particle size measurement in intravenous fluids.

    Science.gov (United States)

    Haines-Nutt, R F; Munton, T J

    1984-08-01

    The level of particulate contamination in a range of large volume injections has been measured using electrical resistance (Coulter) and light blockage (HIAC) techniques. Particle counts showed large variations between the two techniques and although a correlation could be shown for ionic solutions no such correlation could be found for sugar containing solutions. Shape factors alone cannot explain these discrepancies but other differences fundamental to the physics of the two measuring techniques play an important part. We conclude that results obtained using one technique cannot be correlated, theoretically or actually, with those obtained from the other technique. PMID:6148396

  9. Size characterization of Mycobacterium bovis BCG (Bacillus Calmette Guérin) vaccine, Tice substrain.

    Science.gov (United States)

    Zhang, A; Groves, M J

    1988-09-01

    Reconstituted, lyophilized, attenuated Mycobacterium bovis, Bacillus Calmette Guérin (BCG) vaccine, Tice substrain, was characterized using a Coulter Multisizer and a HIAC/Royco counter. The primary organism has an equivalent spherical diameter approximating 1 micron but the BCG cell suspension is heavily aggregated. The cumulative size distribution of the suspension fits a log-probit plot and this information can be used to determine the total number of particles per ampoule. The instrumental count may be related to the viable count. The state of dispersion was unaffected by mild shear (syringe aspiration or ultrasound) and only slightly affected by the addition of cetylpyridinium chloride or sodium tauroglycolate. PMID:3073388

  10. A comparison of assays for the response of primary human T-cells upon stimulation with interleukin-2, interleukin-4 and interleukin-7

    OpenAIRE

    Duschl, Albert; Jahn, Ute; Bertling, Claudia; Sebald, Walter

    2013-01-01

    The most commonly used assay to quantitate the response of peripheral T~cells upon stimulation with growth factors is determination of incorporated (JH]TdR. We compared thls test to three other methods: 1. direct countlog of cells with a Coulter type counter as reference assay, 2. a colorimetric assay using the tetrazolium dye 3-[ 4,S-dimethylthiazol-l-yl]-2,5diphenyl tetrazolium (MTT), which is a cheap and increasingly popular non-radioactive method and 3. incorporation of the thymidine anal...

  11. Cell type-dependent uptake, localization, and cytotoxicity of 1.9 nm gold nanoparticles

    OpenAIRE

    Coulter JA; Jain S; Butterworth KT; Taggart LE; Dickson GR; McMahon SJ; Hyl; WB; Muir MF; Trainor C; Hounsell AR; O'Sullivan JM; Schettino G.; Currell FJ; Hirst DG

    2012-01-01

    Jonathan A Coulter,1 Suneil Jain,2 Karl T Butterworth,2 Laura Taggart,2 Glenn Dickson,2 Stephen J McMahon,3 Wendy Hyland,1 Mark F Muir,3 Coleman Trainor,2 Alan Hounsell,2,4 Joe M O'Sullivan,2,4 Giuseppe Schettino,2 Fred Currell,3 David G Hirst,1 Kevin M Prise21School of Pharmacy, McClay Research Centre, 2Centre for Cancer Research and Cell Biology, 3School of Mathematics and Physics, Queens University Belfast, 4Belfast Health and Social Care Trust, Belfast, IrelandBackground: This fol...

  12. Determinação espectrofotométrica de cálcio em solos Spectrophotometric determination of calcium in soils

    Directory of Open Access Journals (Sweden)

    Fernando A. Soares Coelho

    1969-01-01

    Full Text Available É apresentado estudo da determinação de cálcio em extratos de solo por espectrofotometria de chama, utilizando o espectrofotômetro Beckman, modelo DU. A composição da chama, as pressões dos gases, as interferências e os métodos para eliminá-las foram estudados. Verificou-se ser imprescindível a eliminação das interferências causadas pelos íons de alumínio, ferro e fosfato nas concentrações que normalmente ocorrem nos solos. A utilização de EDTA a pH 9 apresentou resultados satisfatórios na eliminação dessas interferências quando em concentração 0,01 M na solução em que foi determinado o cálcio.A study is presented on the determination of calcium in soil extracts by flame photometry, using a Beckman Model DU spectrophotometer. Flame composition, gas pressure, interferences and their elimination were studied. Results showed it to be necessary to eliminate interferences due to aluminum, iron and phosphate ions in amounts normally present in soils. Enough EDTA solution at pH 9.0 was added to the soil extract until a concentration of 0.01 M in EDTA was reached.

  13. Contornos de entonación en el discurso de una docente. Su relación con algunos aspectos pragmáticos

    Directory of Open Access Journals (Sweden)

    Guadalupe Álvarez

    2005-06-01

    Full Text Available En este artículo se describen los contornos de los grupos entonacionales del discurso de una docente con el objeto de determinar las características que adoptan en relación con algunos aspectos pragmáticos relevantes de ese discurso, como partículas de iniciación, continuidad, completud y diferentes actos de habla (aseveraciones, órdenes, pedidos o sugerencias y, sobre todo, distintas clases de preguntas. Para ello, realizo una descripción perceptual y físico-acústica de 172 grupos entonacionales combinando el sistema TOBI de notación (Beckman y Ayers Elam, 1993 con la marcación de valores ERB y la posición silábica (Gurlekian y otros, 1999. (The aim of this article is describe the contours of the intonational groups of a teacher’s discourse in order to determine the characteristics that they adopt in relation to some relevant pragmatic aspects of it, included initial particles, continuity, completion and different speech acts. To achieve this, I describe 172 intonational groups from an auditory and physical-acoustic analyses, combining the TOBI system notation (Beckman y Ayers Elam, 1993 with the ERB-rate scale and the syllabic position (Gurlekian et al, 1999.

  14. Comparison and harmonization assessment of mutual among four different immunoassay systems in CA19-9 test%CA19-9测定在4个免疫检测系统的差异及互认能力的评估

    Institute of Scientific and Technical Information of China (English)

    宋超; 张曙云; 周俊; 单志明; 周永列

    2013-01-01

    目的 研究4个免疫检测系统对CA19-9检测的差异和一致性,探讨实现CA19-9检测结果互认的基础.方法 检测系统比对研究.汇集整理CA19-9室间质量评价活动批号的数据(卫生部200811 ~ 201215,浙江省080309 ~ 120211);搜集120份不同CA19-9浓度的新鲜血样本;搜集2010年9月至2012年3月不同检测系统非肿瘤体检人群实测CA19-9数据.选择4个稳定的检测系统Abbott Architect i2000、Roche E170、Beckman Dxi800和Siemens CentaurXP作为研究对象,统计分析在卫生部和浙江省室间质评(冻干粉和液体血清)中的组间差异;参照CLSI EP9-A2进行120份新鲜血样本的比对分析;计算各检测系统实测CA19-9的95%置信区间并分析.采用散点图、加权回归方程进行统计比较.结果 室间质评与新鲜血比对出现颠倒的结果.室间质评均表现出检测系统组间较大的偏倚,斜率bw在1.340到4.683之间,相关系数r较好;新鲜血比对中r不理想,但斜率bw接近于1.新鲜血比对中在推荐浓度27 U/ml时,检测系统两两间偏移分别为Abbott-Roche(-6.41%),Beckman-Roche(-5.07%),Siemens-Roche(13.15%),Beckman-Abbott(2.46%),Siemens-Abbott(22.52%),Siemens-Beckman(39.66%).部分检测系统间实测95%置信区间差异有统计学意义.结论 在研究检测系统差异时,在较低浓度CA19-9的结果在4个检测系统间具有互认的基础,但在高浓度时,检测系统间的差异明显增大,风险较高,尚无法实现互认.%Objective To investigate the differences and harmonization of immunoassay systems in detecting CA19-9 and to assess the possibility of mutual recognition in different laboratories.Methods Data were collected and analyzed from External Quality Assessments (EQA) of NCCL(Lots:200811-201215) and ZJCCL(Lots:080309-120211).120 fresh serum with different concentrations of CA19-9 were collected.The CA19-9 results of healthy people were also collected from September 2010 to March

  15. Trueness investigation of routine creatinine assays on nine homogeneous systems in Beijing demonstrates an encouraging outcome that meets clinical requirements

    Institute of Scientific and Technical Information of China (English)

    LIU Yan; XU Guo-bin

    2010-01-01

    Background Serum creatinine (Scr) measurement plays a key role in glomerular filtration rate estimation (eGFR),chronic kidney disease (CKD) diagnosis as well as CKD treatment. However, the test results of Scr from different laboratories vary significantly. In order to get comparable results, the European in vitro diagnostic (IVD) directive requires traceability to reference methods and materials. The purpose of this study was to verify the effect of traceability implementation by investigating the trueness of creatinine measurement on nine homogenous systems in Beijing. Methods Commutable frozen human serum reference material, National Institute of Standards & Technology (NIST) Standard Reference Material (SRM) 967, was used to verify the trueness of Scr measurement results from nine homogeneous analytical systems of seven companies which are the most widely used systems in Beijing's third-grade hospitals. The methods referred to the Jaffe's and Enzymatic methods. Results from nine routine measurement systems were assessed using two criteria: biological variability and Clinical Laboratory Improvement Amendments' 88 (CLIA' 88).We simulated a series of broken lines representing the limits of SD and bias that would produce a relative increase (or decrease) of 10% and 20% in the measurement error when estimating GFR (MEeGFR) using the isotope dilution mass spectrometry (IDMS)-traceable Modification of Diet in Renal Disease (MDRD) Study equation. Results of the College of American Pathologists (CAP) 2008-B LN24 Survey were compared with our investigation results. Results Compared with the total error criteria of biological variability, Ortho (traceable to IDMS) met the minimum acceptable criteria; Roche (Jaffe), Roche (Enzymatic), Shino and Daiichi met the desirable criteria at level Ⅰ. At level Ⅱ, Ortho (traceable to gas chromatography/isotope dilution mass spectrometry, GC/IDMS), Dade Behring and Beckman (traceable to rate Jaffe) met the minimum acceptable

  16. Expert Assistant For A Clinical Hematology Blood Cell Analyzer

    Science.gov (United States)

    Young, Carole; Navlakha, Jainendra K.

    1989-03-01

    The COULTER COUNTER Model S Plus Series instruments are automated clinical hematology blood cell analyzers which measure the count, volume and population distribution of red blood cells, white blood cells and platelets, and hemoglobin from patient blood samples. In the clinical laboratory environment, instrument startup consists of a number of component and system checks to assure proper operation and calibration to insure reliable results are produced on patient samples. If a startup check fails, troubleshooting procedures are provided to assist the operator in determining the cause of the error. Troubleshooting requires expertise in instrument operation, troubleshooting procedures and evaluation of the data produced. This expert system is designed and developed to assist the startup diagnostics of COULTER COUNTER Model S Plus Series instruments. The system reads data produced by the instrument and validates it against expected values. If the values are not all correct, then the troubleshooting starts. Troubleshooting is handled for the most common subsystem problems and those which the operator has the equipment and knowledge to handle, problems that are cheapest to fix and problems that are quickest to fix. The expert system restarts the startup sequence whenever troubleshooting has been successful or recommends calling Customer Service when unsuccessful.

  17. Reaching out to the American middle class: Peggy Noonan’s conservatism

    Directory of Open Access Journals (Sweden)

    Luc BENOIT A LA GUILLAUME

    2008-10-01

    Full Text Available Parmi les idéologues qui popularisent les idées conservatrices auprès du grand public, Peggy Noonan occupe une place intéressante. Moins extrémiste que Rush Limbaugh ou Ann Coulter, elle exprime les angoisses et les espoirs des Reagan Democrats, ces Américains moyens devenus conservateurs depuis les années 80. Cet article analyse les ambivalences d’un discours qui entre en résonance avec celles d’une partie de la classe moyenne américaine.Since the 1990s, Peggy Noonan’s writings have translated conservative ideas into articles and bestselling books for the wider American public. In a less abrasive way than Rush Limbaugh or Ann Coulter, she expresses the hopes and fears of the Reagan Democrats. This article analyzes Noonan’s ambivalent rhetoric, which mirrors the ambivalent attitudes of parts of America’s embattled middle class.

  18. Modifikasi Mesin Penyiang dan Penggulud Tipe Bajak Dua Sayap dan Uji Kinerja pada Tiga Jenis Tanah

    Directory of Open Access Journals (Sweden)

    Gatot S.A Fatah

    2008-08-01

    Full Text Available The machine performance test of power weeder in the heavy soil shows that: Low cannot penetration, High rolling resistance and Gear box was very hot. The objectives research were: 1. Modification the power weeder for applied to the diversity texture of soil, 2. to know performance tne rubber wheel compare to iron wheel and 3. to know performance test the coulter. The methods used are: 1 to measure the land condition, such as: moisture content of soil, penetratIon resistance, shear stress, average mass diameter and roughness surface. 2 to test machine performance, such as: weeded percentage, rolling wheel, forward velocity, gasoline consumed and weeded capacity in the tree researches located. The result of the research were: 1 Design of the iron wheel can be applied to three kind of soil (light, moderate and heavy soil. In the heavy soil (Kendalpayak weed percentage 58.33%, fuel consumption 1.231 hour1 and weed capacity was 13.17 hours ha-1; 2 Design of the rubber wheel can applied in the light soil (Muneng; 3. Coulter can be applied in ligh and moderate soil for the moisture content of soil was 21.70%.

  19. Fuel consumption, labour requirement and capacity on reduced tillage and direct seeding; Braendstofforbrug, arbejdsforbrug og kapacitet ved reduceret jordbearbejdning og direkte saening

    Energy Technology Data Exchange (ETDEWEB)

    Nielsen, V.; Mortensen, H.; Soerensen, K. [Danmarks JordbrugsForskning. Afd. for Jordbrugsteknik (Denmark)

    2004-06-01

    A project which is partly financed by the Danish Energy Agency, was initiated in the autumn of 2001 with the objective of investigating the energy saving potentials involved with the establishment of crops by use of the latest technology. Most of the findings were made at test farms, where fuel meters and chronometers were mounted on the tractors. In addition to this, the tractor drivers would note down digures for area, working depth, soil type, ground and weather conditions, etc. Besides that, technicians from research Centre Bygholm have made a few time studies and measurements on fuel consumption. The following machine types were used for the studies: Single disc coulter drills; Hoe tine drills; Stubble cultivators with wing sweeps; Finnish rotary harrow; Disc harrow; Straw spreader. Results from previous surveys on traditional crop establishment where rotary and compact harrow units were used are also included in the survey. In the case of reduced tillage, the fuel consumption can be reduced by 33-64%, depending on the method and technique used. In the case of direct drilling, the fuel consumption can be reduced by about 70%. The labour requirement and the CO{sub 2} emission can be reduced by nearly the same rates per cent. The fuel consumption is not uniquely lower on sandy soil than on clay soil. Contrary to what would be the case when seeding with Horsch hoe coulter drill with duet wing sweeps, the differences involved with seeding with Vaederstad single disc coulter drill and Koeckerling hoe tine drill with wing sweeps will not be determined by the soil type. When a single disc coulter drill (Kulti-Seeder or Vaederstad) is use for seeding, it is quite common to plough the soil in advance. However, no significant differnces in the fuel consumption were seen, whether or not the soil had been ploughed or harrowed prior to seeding. Significantly lower fuel consumption on seeding was seen for the Dublet Record Kulti-Seeder than for the other seed drills. No

  20. Myxomycetes do Parque Zoobotânico de Teresina, Piauí, Brasil Myxomycetes of the Parque Zoobotânico - Teresina, Piauí, Brazil

    Directory of Open Access Journals (Sweden)

    Márcia Percília Moura Parente Ponte

    2003-03-01

    Full Text Available Visando ampliar o conhecimento sobre a mixobiota do Piauí, foram realizadas coletas no Parque Zoobotânico de Teresina (05º 05' 12'' S, 42º 48' 48'' WG. A vegetação do Parque caracteriza-se como um cerradão com representantes da mata de cocais, que oferecem substratos potencialmente favoráveis ao desenvolvimento de Myxomycetes. Esporocarpos foram coletados no período chuvoso e início da estiagem (março a junho de 1999, identificados e depositados no herbário UFP (Universidade Federal de Pernambuco. Foram obtidas 101 amostras, correspondentes a 19 espécies, distribuídas nas famílias Ceratiomyxaceae (Ceratiomyxa fruticulosa (Mull. Macbr., Clastodermataceae (Clastoderma debaryanum Blytt, Cribrariaceae (Cribraria microcarpa (Schrad. Pers., C. violacea Rex, Dictydium canceilatum* (Batsch. Macbr., Enteridiaceae (Lycogala exiguum* Morgan., Physaraceae (Craterium aureum* (Schum. Rost., Physarum bogoriense* Racib., P. melleum (Berk. & Br. Massee, P. nucleatum Rex, P. pusilium* (Berk. & Curt. G. Lister, Trichiaceae (Arcyria denudata (L. Wett., A. cinerea (Bull. Pers., Hemitrichia calyculata (Speg. Farr, H. serpula (Scop. Rost., Metatrichia vesparium (Batsch Nann.-Brem., Perichaena depressa Lib. e Stemonitaceae (Comatricha typhoides (Bull. Rost., Stemonitis smithii Macbr.. Exceto H. calyculata e H. serpula, todos os táxons são referidos pela primeira vez para o município de Teresina; as espécies assinaladas com um asterisco constituem novos registros para o Piauí.Aiming to increase the knowledge about the myxobiota of Piauí State, Brazil, collections were done in the undertaken Zoobotanical Park of Teresina (05° 05' 12'' S, 42° 48' 48'' WG. The vegetation of the Park is a savanna ("cerradão" with "cocais" forest representative, offering substrates potentially favorable to the development of Myxomycetes. Sporocarps were collected in the rainy season (March to June 1999, identified and deposited at the UFP herbarium (Federal University

  1. Initial soil respiration response to biomass harvesting and green-tree retention in aspen-dominated forests of the Great Lakes region

    Science.gov (United States)

    Kurth, Valerie J.; Bradford, John B.; Slesak, Robert A.; D'Amato, Anthony W.

    2014-01-01

    Contemporary forest management practices are increasingly designed to optimize novel objectives, such as maximizing biomass feedstocks and/or maintaining ecological legacies, but many uncertainties exist regarding how these practices influence forest carbon (C) cycling. We examined the responses of soil respiration (Rs) to biomass harvesting and green-tree retention in an effort to empirically assess their impacts on C cycling. We measured Rs and soil microclimatic variables over four growing seasons following implementation of these management practices using a fully replicated, operational-scale experiment in aspen-dominated forests in northern Minnesota. Treatments included three levels of biomass removal within harvested areas: whole-tree harvest (no slash deliberately retained), 20% slash retained, and stem-only harvest (all slash retained), and two levels of green-tree retention: 0.1 ha aggregate or none. The relative amount of biomass removed had a negligible effect on Rs in harvested areas, but treatment effects were probably obscured by heterogeneous slash configurations and rapid post-harvest regeneration of aspen in all of the treatments. Discrete measurements of Rs and soil temperature within green-tree aggregates were not discernible from surrounding harvested areas or unharvested control stands until the fourth year following harvest, when Rs was higher in unharvested controls than in aggregates and harvested stands. Growing season estimates of Rs showed that unharvested control stands had higher Rs than both harvested stands and aggregates in the first and third years following harvest. Our results suggest that retention of larger forest aggregates may be necessary to maintain ecosystem-level responses similar to those in unharvested stands. Moreover, they highlight the innate complexity of operational-scale research and suggest that the initial impacts of biomass harvest on Rs may be indiscernible from traditional harvest in systems where incidental

  2. Soil and vegetation changes after clear-felling coniferous forests: effects of varying removal of logging residues

    International Nuclear Information System (INIS)

    Effects of the intensity of logging residue harvesting on soil nutrient status and ground vegetation cover were examined over a 16-year period in two series of field experiments in Sweden. Short-term effects of slash harvesting and stump removal on soil water chemistry were studied after clear-felling a Norway spruce (Picea abies (L.) Karst.) stand in SW Sweden. Soil water concentrations of NH4+, and NO3- and K+ were lower shortly after whole-tree harvesting (i.e. stem and slash harvesting) than shortly after conventional stem-only harvesting or complete tree harvesting (i.e. stem, slash and stump removal). However, 5 years later there were no longer differences in nutrient concentrations detected between treatments, and nutrient levels approached those normally found in drainage water from forest land. Similar studies focussed on long-term (16 years) effects were conducted on four coniferous forest sites in Sweden, two in north and the other two in the south. In each region one site was situated in a pure Scots pine stand (Pinus sylvestris L.) and the other in a pure Norway spruce stand. In general, the intensity of slash harvesting had no effect on the total pools of nitrogen or carbon in the soil. Furthermore, this study showed experimentally that the harvesting of logging residues results in long-term soil acidification and depletions of exchangeable base cations, manganese and zinc pools, which lead in turn to a reduction in base saturation levels. A major implication for practical forestry was that guidelines and recommendations concerning the large-scale utilization of logging residues should be based more on the nutritional and soil acidifying consequences of this practice than on its potential effect on soil organic matter storage. It would also be possible to mitigate the detrimental effects that slash harvesting has on site conditions by applying wood-ash or other nutrients in inorganic form. 53 refs, 4 figs, 4 tabs

  3. Phylogeny and taxonomic revision of Microascaceae with emphasis on synnematous fungi.

    Science.gov (United States)

    Sandoval-Denis, M; Guarro, J; Cano-Lira, J F; Sutton, D A; Wiederhold, N P; de Hoog, G S; Abbott, S P; Decock, C; Sigler, L; Gené, J

    2016-01-01

    The taxonomy of the synnematous genera Cephalotrichum, Doratomyces and Trichurus, and other related genera Gamsia, Wardomyces and Wardomycopsis, has been controversial and relies mainly on morphological criteria. These are microascaceous saprobic fungi mostly found in air and soil and with a worldwide distribution. In order to clarify their taxonomy and to delineate generic boundaries within the Microascaceae, we studied 57 isolates that include clinical, environmental and all the available ex-type strains of a large set of species by means of morphological, physiological and molecular phylogenetic analyses using DNA sequence data of four loci (the ITS region, and fragments of rDNA LSU, translation elongation factor 1α and β-tubulin). The results demonstrate that Cephalotrichum, Doratomyces and Trichurus are congeneric and the genus Cephalotrichum is accepted here with Echinobotryum as a further synonym. The genera Acaulium and Fairmania, typified by A. albonigrescens and F. singularis, respectively, are distinct from Microascus and Scopulariopsis, Gamsia is distinct from Wardomyces, and Wardomycopsis is confirmed as a separate genus in the Microascaceae. Two new species of Cephalotrichum are described as C. brevistipitatum and C. hinnuleum. Nine new combinations are proposed, i.e. Acaulium acremonium, A. caviariforme, Cephalotrichum asperulum, C. columnare, C. cylindricum, C. dendrocephalum, C. gorgonifer, Gamsia columbina and Wardomyces giganteus. A neotype is designed for C. stemonitis. Lectotypes and epitypes are designated for A. acremonium, A. albonigrescens, C. gorgonifer, C. nanum and W. anomalus. Cephalotrichum cylindricum, C. microsporum, F. singularis and Gamsia columbina are also epitypified with new specimens. Descriptions of the phenotypic features and dichotomous keys for identification are provided for accepted species in the different genera. PMID:27616803

  4. Impacts of forest harvesting on mobilization of Hg and MeHg in drained peatland forests on black schist or felsic bedrock.

    Science.gov (United States)

    Ukonmaanaho, Liisa; Starr, Mike; Kantola, Marjatta; Laurén, Ari; Piispanen, Juha; Pietilä, Heidi; Perämäki, Paavo; Merilä, Päivi; Fritze, Hannu; Tuomivirta, Tero; Heikkinen, Juha; Mäkinen, Jari; Nieminen, Tiina M

    2016-04-01

    Forest harvesting, especially when intensified harvesting method as whole-tree harvesting with stump lifting (WTHs) are used, may increase mercury (Hg) and methylmercury (MeHg) leaching to recipient water courses. The effect can be enhanced if the underlying bedrock and overburden soil contain Hg. The impact of stem-only harvesting (SOH) and WTHs on the concentrations of Hg and MeHg as well as several other variables in the ditch water was studied using a paired catchment approach in eight drained peatland-dominated catchments in Finland (2008-2012). Four of the catchments were on felsic bedrock and four on black schist bedrock containing heavy metals. Although both Hg and MeHg concentrations increased after harvesting in all treated sites according to the randomized intervention analyses (RIAs), there was only a weak indication of a harvest-induced mobilization of Hg and MeHg into the ditches. Furthermore, no clear differences between WTHs and SOH were found, although MeHg showed a nearly significant difference (p = 0.06) between the harvesting regimes. However, there was a clear bedrock effect, since the MeHg concentrations in the ditch water were higher at catchments on black schist than at those on felsic bedrock. The pH, suspended solid matter (SSM), dissolved organic carbon (DOC), and iron (Fe) concentrations increased after harvest while the sulfate (SO4-S) concentration decreased. The highest abundances of sulfate-reducing bacteria (SRB) were found on the sites with high MeHg concentrations. The biggest changes in ditch water concentrations occurred first 2 years after harvesting. PMID:26979172

  5. Screening and identification of the antagonistic organisms against Colletotrichum coccodes on potato%马铃薯炭疽病(Colletotrichum coccodes)拮抗菌株的筛选及鉴定

    Institute of Scientific and Technical Information of China (English)

    王涵琦; 畅涛; 杨成德; 陈秀蓉

    2014-01-01

    采用对峙培养法对马铃薯炭疽病的拮抗菌株进行了筛选和鉴定.结果表明,拮抗菌株ZHA10对炭疽病病原菌具有良好的抑制作用,抑制率达76.43%,其可以使病原菌菌丝变形和裂解;ZHA10菌体短杆状、革兰氏阳性、大小为(0.5~0.75)μm×(1.5~3.1)μm、芽胞中至尖端生.通过生理生化测定、16S rDNA以及gyrB序列分析,鉴定ZHA10为枯草芽胞杆菌(Bacillus subtilis).此外,ZHA10还对番茄早疫病菌(Alternaria solani)、马铃薯干腐病菌(Fusarium oxysporum)、孜然根腐病菌(Fusarium solani)、小麦根腐病菌(Bipolaris sorokiniana)、黄瓜枯萎病菌(Fusarium oxysporum)和马铃薯褐腐病菌(Stysanus stemonitis)均具有较强的拮抗作用.ZHA10抑菌能力良好,抑菌谱宽,具有进一步开发应用的潜力.

  6. Fifteen-year patterns of soil carbon and nitrogen following biomass harvesting

    Science.gov (United States)

    Kurth, Valerie J.; D'Amato, Anthony W.; Palik, Brian J.; Bradford, John B.

    2014-01-01

    The substitution of forest-derived woody biofuels for fossil fuel energy has garnered increasing attention in recent years, but information regarding the mid- and long-term effects on soil productivity is limited. We investigated 15-yr temporal trends in forest floor and mineral soil (0–30 cm) C and N pools in response to organic matter removal treatments (OMR; stem-only harvest, SOH; whole-tree harvest, WTH; and whole-tree plus forest floor removal, FFR) at three edaphically distinct aspen (Populus tremuloides Michx. and P. grandidentata Michx.) forests in the Great Lakes region. The OMR and temporal effects were generally site specific, and both were most evident in the forest floor and combined profile (mineral soil and forest floor) compared with the mineral soil alone. Forest floor and combined profile C and N pools were generally similar in the SOH and WTH treatments, suggesting that slash retention has little impact on soil C and N in this time frame. Temporal changes in C and N at one of the three sites were consistent with patterns documented following exotic earthworm invasion, but mineral soil pools at the other two sites were stable over time. Power analyses demonstrated that significant effects were more likely to be detected for temporal differences than the effects of OMR and in the combined profile than in the mineral soil. Our findings are consistent with previous work demonstrating that OMR effects on soil C and N pools are site specific and more apparent in the forest floor than the mineral soil.

  7. Carbon and nitrogen pools and mineralization rates in boreal forest soil after stump harvesting

    Science.gov (United States)

    Kaarakka, Lilli; Hyvönen, Riitta; Strömgren, Monika; Palviainen, Marjo; Persson, Tryggve; Olsson, Bengt A.; Helmisaari, Heljä-Sisko

    2016-04-01

    The use of forest-derived biomass has steadily increased in the Finland and Sweden during the past decades. Thus, more intensive forest management practices are becoming more common in the region, such as whole-tree harvesting, both above- and belowground. Stump harvesting causes a direct removal of carbon (C) in the form of biomass from the stand and can cause extensive soil disturbance, which in turn can result in increased C mineralization. In this study, the effects of stump harvesting on soil C and nitrogen (N) mineralization, and soil surface disturbance were studied at two different clear-felled Norway spruce (Picea abies) stands in Central Finland. The treatments were conventional stem-only harvesting combined with mounding (WTH) and stump harvesting (i.e. complete tree harvesting) combined with mounding (WTH+S). Logging residues were removed from all study sites. Soil samples down to a depth of 20 cm were systematically collected from the different soil disturbance surfaces (undisturbed soil, the mounds and the pits) 12-13 years after final harvest. Soil samples were incubated in the laboratory to determine the C and N mineralization rates. In addition, total C and N pools were estimated for each disturbance class and soil layer. Soil C and N pools were lower following stump harvesting, however, no statistically significant treatment effect was detected. Instead, C mineralization responses to treatment intensity was site-specific. C/N-ratio and organic matter content were significantly affected by harvest intensity. The observed changes in C and N pools appear to be related to the intrinsic variation of the surface disturbance and soil characteristics, and harvesting per se, rather than treatment intensity. Long-term studies are however needed to draw long-term conclusions whether stump harvesting significantly changes soil C and nutrient dynamics.

  8. A High-affinity Activator of G551D-CFTR Chloride Channel Identified By High Throughput Screening

    Institute of Scientific and Technical Information of China (English)

    ZHAO Lu; HE Cheng-yan; LIU Yan-li; ZHOU Hong-lan; ZHOU Jin-song; SHANG De-jing; YANG Hong

    2004-01-01

    A stably transfected CHO cell line coexpressing G551D-CFTR and iodide-sensitive yellow fluorescent protein mutant EYFP-H148Q-I152L was successfully established and used as assay model to identify small-molecule activators of G551D-CFTR chloride channel from 100000 diverse combinatorial compounds by high throughput screening on a customized Beckman robotic system. A bicyclooctane compound was identified to activate G551D-CFTR chloride channel with high-affinity(Kd=1.8 μmol/L). The activity of the bicyclooctane compound is G551D-CFTR-specific, reversible and non-toxic. The G551D-CFTR activator may be useful as a tool to study the mutant G551D-CFTR chloride channel structure and transport properties and as a candidate drug to cure cystic fibrosis caused by G551D-CFTR mutation.

  9. Enzymatic assay of total cholesterol in serum or plasma by amperometric measurement of rate of oxygen depletion following saponification.

    Science.gov (United States)

    Kumar, A; Christian, G D

    1977-01-17

    A method for serum or plasma cholesterol assay involving amperometric measurement of the rate of oxygen depletion in the cholesterol oxidase-catalyzed oxidation of cholesterol is described. The hydrolysis of the serum cholesterol esters is accomplished by saponification of 50 mul of sample with 0.2 ml of ethanolic KOH (1.0 mol/1) containing 0.5% Triton X-100 for 5 min at 75 degrees C. The rate of oxygen consumption in a 25-mul aliquot of this is measured with a Clark electrode in a Beckman Glucose Analyzer and the assay takes about one minute after incubation; results are read digitally on the instrument. The analyzer cell contains 1 ml of 1 M phosphate buffer, pH 7.4, with 100 mg sodium cholate/100 ml and 0.1-0.2 U cholesterol oxidase.

  10. Biocromos (pigmentos de invertebrados marinhos: I - Briozoarios

    Directory of Open Access Journals (Sweden)

    Gilberto G. Villela

    1948-06-01

    Full Text Available In the present paper the author reported the results obtained for the chemical caracterization of the pigments of some species of Bryozoa (Polyzoa, living in the bay and the coast near Rio de Janeiro. The pigments (biochromes of "Bugula neritina", "Schizoporella unicornis", "Steganoporella magnilabris", "Bugula flabellata" and "Trigonospora sp", were extracted and the results showed that carotene was found in all the species, except, "Bugula neritina" and "Bugula flabellata". A new water-soluble pigment was described for "Bugula neritina". Spectrophotometric curves obtained with the Beckman spectrophotometer are reported for the "Bugula" pigment and for the carotenoids. Chromatographic analysis and the treatment with immiscible solvents were performed for all the extracts of the animals considered. A study of the biochromes of other "phyla" will be published in a near future.

  11. Resolution of two surface glycoproteins from human parainfluenza-3 virus by crossed immunoelectrophoresis.

    Science.gov (United States)

    Holling, R A; Guskey, L E

    1984-07-01

    The technique of two-dimensional crossed immunoelectrophoresis (CIE) was used to resolve two glycoproteins from purified human parainfluenza type 3 virus. Virus preparations were extracted with Triton X-100 and fractionated by centrifugation in a Beckman airfuge. Two immunoprecipitates were detected by CIE in the supernatant fractions, but were not found in the pellets from extracted virus. Viral glycoproteins labeled with [35S]methionine were isolated by affinity chromatography on concanavalin A (Con A) agarose columns, resolved by CIE and detected by autoradiography. Resolution of two glycoprotein peaks from as little as 4.5 micrograms of protein from extracted virus is consistent with results from polyacrylamide gel patterns showing two unique glycoproteins with molecular weights of 48 kd and 65 kd. PMID:6088566

  12. Exploring variation in accuracy and contrast for sibilant fricatives at the onset of fricative acquisition

    Science.gov (United States)

    Nicholson, Hannele Buffy Marie

    Children's speech differs from adult speech in the many ways, including in its phonetic characteristics. A central question for researchers interested in child speech sound acquisition is when and how a child acquires robust adult-like contrasts. In this thesis, I present a protocol for the analysis of the English sibilant fricatives "s" and "sh". Sibilant fricatives are of interest because they are late-acquired sounds that require articulatory-aerodynamic coordination, and are contrastively necessary in multiple languages around the world, English especially. Given the turbulent nature of the sound spectrum of fricative consonants, few agreed upon measures exist. Holliday, Reidy, Beckman and Edwards (In Preparation) propose that peak equivalent rectangular bandwidth is a psychoacoustically appropriate measure for modeling the robustness of phonological contrast between sibilant fricative types. The robustness measures put forth by Holliday et al. are applied to data from the speech of toddlers aged 28-39 months and are discussed.

  13. Determination of free and total carnitine with a random-access chemistry analyzer.

    Science.gov (United States)

    Wan, L; Hubbard, R W

    1998-04-01

    Carnitine deficiency presents as a major problem in fatty acid oxidation. The use of a plasma carnitine assay can rapidly help to describe this deficiency. The method we describe here requires two simple steps of sample preparation, followed by automated analysis with the Beckman Synchron CX4 random-access chemistry analyzer. The goal of this method development was to reduce the cost of analysis and to allow a greater number of laboratories to perform this assay on demand within 1 h for both free and total carnitine. The method has a linearity of 0-150 micromol/L and a detection limit of 5 micromol/L. The inter- and intraday CVs are <20%. The method agreed closely with both the widely used RIA and spectrophotometric methods.

  14. Reproducing sterile neutrinos and the behavior of flavor oscillations with superconducting-magnetic proximity effects

    Science.gov (United States)

    Baker, Thomas E.

    2016-03-01

    The physics of a superconductor subjected to a magnetic field is known to be equivalent to neutrino oscillations. Examining the properties of singlet-triplet oscillations in the magnetic field, a sterile neutrino is suggested to be represented by singlet Cooper pairs and moderates flavor oscillations between three flavor neutrinos (triplet Cooper pairs). A superconductor-exchange spring system's rotating magnetization profile is used to simulate the mass-flavor oscillations in the neutrino case and the physics of neutrino oscillations are discussed. Connecting the condensed matter system and the particle physics system with this analogy may allow for the properties of the condensed matter system to inform neutrino experiments. Support is graciously acknowledged from the Pat Beckman Memorial Scholarship from the Orange County Chapter of the Achievement Rewards for College Scientists Foundation.

  15. Is the Beckmann rearrangement a concerted or stepwise reaction? A computational study.

    Science.gov (United States)

    Yamabe, Shinichi; Tsuchida, Noriko; Yamazaki, Shoko

    2005-12-23

    [reaction: see text] RB3LYP calculations were performed on the Beckman rearrangement by the use of three substrates, acetone oxime (1), acetophenone oxime (2), and cyclohexanone oxime (3). Acidic solvents were modeled by H+ (CH3COOH)3 and H3O+ (H2O)6, and reaction paths were determined precisely. For 1, a two-step process involving a sigma-type cationic complex was obtained. For 2, a three-step process with pi- and sigma-type complexes was found in H+ (CH3COOH)3 and a two-step process involving a sigma-type cationic complex was obtained in H3O+ (H2O)6. However, for 3, a concerted process without pi and sigma complexes was calculated, which leads to the product, epsilon-caprolactam. Three different mechanisms were explained in terms of FMO theory.

  16. 三种CA125检测系统的一致性评价%Measuring agreement in comparison of CA125 determined by three different immunoassay systems

    Institute of Scientific and Technical Information of China (English)

    张巧娣; 谢而付; 张燕; 凌芸; 蒋理; 徐华国

    2015-01-01

    Objective:To assess the agreement of three different chemiluminescent detection system of CA125 measurement by using Bland-Altman analysis.Methods:One hundreds serum samples were obtained from 100 patients. CA125 of these samples were measured by three different chemiluminescent detection system, including Roche, Beckman and Siemens. Bland-Altman analysis and Spearman correlation analysis were performed to assess the results of every two systems, and draw the limits of agreement as an evaluation of agreement of different systems. Results:The distribution of CA125 results of Roche, Beckman and Siemens system were 5.6~279.8, 4.5~542.4 and 4.5~350.0 U/mL, respectively. Spearman correlation analysis showed that the results of every two methods had good correlation, the correlation coeffcient were 0.932 (P=0.000) between Roche and Beckman, 0.947 (P=0.000) between Roche and Siemens, 0.948 (P=0.000) between Beckman and Siemens. Bland-Altman analysis for the measurements showed bad relativity. The Bland-Altman plot revealed lower agreement between every two methods for CA125 results.Conclusion:Three detection systems do not have the consistency of the CA125 results, it is recommended to establish a standardized reference system of CA125 for comparison.%目的:使用Bland-Altman分析法,评估三种化学发光检测系统测定血清糖链抗原125(cancerantigen,CA125)结果之间的一致性.方法:选取100例患血清,分别使用Roche、Beckman和Siemens公司的检测系统进行CA125检测,对每两种检测系统之间的检测结果进行相关性分析与Bland-Altman分析,并绘制一致性限度作为评价一致性的指标.结果:Roche、Beckman和Siemens检测系统检测CA125结果范围分别为5.6~279.8、4.5~542.4和4.5~350.0U/mL;不同检测系统间相关系数如下:Roche与Beckman为0.932(P=0.000),Roche与Siemens为0.947(P=0.000),Beckman与Siemens为0.948(P=0.000),每两组之间均存在相关;Bland-Altman一致性

  17. STIMULATIONG EFFECT OF LOW DOSE 147Pm ON DNA REPAIR IN SPERMIOGENIC STAGES

    Institute of Scientific and Technical Information of China (English)

    朱寿彭; 王六一

    1995-01-01

    For labelling spermatid DNA,male BALB/c mice are injected intratesticularyly with 3H-TdR in advance.After 36d of labelling,spermatozoa are sampled which is just the time required for the germ cells to mature.The period from intraperitoneal injection of 147Pm to sampling spermatozoa is 12d.After sperm cells have been lysed.30ml eluent is added by wriggle pump.Finally,both the DNA remained on the filters and filtrated bottles are determined with a Beckman liquid scintillation device.Results show that after small dose internal irradiation with 185Bq/g of 147Pm,an increase in amount of sperm DNA on the filter observed is considerably higher than the control group.This indicates that low level internal radiation of 147Pm has tendency to stimulate the DNA repair in spermiogenic stages.

  18. Applicability of a prototype for determination of absorbed dose using brachytherapy equipment with Ir-192 sources; Aplicabilidade de um prototipo para determinacao da dose absorvida utilizando equipamentos de braquiterapia com fontes de IR-192

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Vivianne Lucia Bormann; Almeida, Mayara Gabriella Oliveira de; Vieira, Rafaela Etelvina de Amorim; Silva, Waldecy Ananias da; Nascimento, Rizia Keila, E-mail: vlsouza@cnen.gov.br, E-mail: mayaradqf@hotmail.com, E-mail: rodriguesss@hootmail.com, E-mail: waldecy@cnen.gov.br, E-mail: riziakeila@hotmail.com [Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil)

    2014-07-01

    This work aims at the development and improvement of a device to perform the absolute dosimetry sources of Ir-192 using the Fricke solution contained in a flask. The Fricke solution used was prepared using amounts of ferrous ammonium sulfate, sodium chloride and sulfuric acid, diluted with water tri distilled pre-established in the literature. The spectrophotometer used was a UV-VIS spectrophotometer (Beckman DU-640 Counter) for measuring the optical density at wavelength 304 nm. The calculation for determining the radial dose takes into account the radial distance and the angle formed with the transverse axis of the source. As the results obtained can be seen that the states of Pernambuco, Ceara, Paraiba e Piaui are in accordance with the recommendations of international standards of the International Atomic Energy Agency (IAEA), which considers not acceptable a difference greater than 5% of prescribed dose and measured dose.

  19. Research on the plasma biochemistry of cardiac function, liver function and renal function from healthy larval green turtles (Chelonia mydas) in captivity%绿海龟健康幼体心肝肾功能血液生化指标参考值研究

    Institute of Scientific and Technical Information of China (English)

    成奇; 潘连德

    2016-01-01

    采集了39只人工养殖的健康幼体绿海龟(1.5~2.5龄)、6只健康成体绿海龟(10~ 20龄)和3只野生成体绿海龟,用Beckman AU5800和TNI BECKMAN Access 2测定和分析了反映绿海龟心肝肾功能的17项血液生化指标,结果发现野生个体的生化指标与人工养殖的绿海龟差异较大,谷草转氨酶(P =0.002)、白蛋白(P =0.015)、碱性磷酸酶(P=O.010)、谷丙转氨酶(P=O.OIO)等均有明显差异,人工养殖绿海龟除碱性磷酸酶与年龄呈负相关外没有明显差异,不同性别的个体在生化指标上差异不显著.本实验结果与野生绿海龟各研究结果对比发现,野生个体的指标差异非常显著,难以作为健康指标的参考.本研究建立了人工绿海龟的心肝肾功能血液生化指标参考值,为野生绿海龟血液生化研究提供参考,为指导绿海龟养殖过程中的疾病早期诊断和预防提供依据.

  20. Comparison of blood glucose test results between point-of-care testing glucose meter and biochemical analyzer%POCT血糖仪与生化分析仪血糖检测结果的比对试验及分析

    Institute of Scientific and Technical Information of China (English)

    刘加红

    2014-01-01

    Objective To explore precision and accuracy of point-of-care testing(POCT) blood glucose meter through comparing blood glucose results between POCT glucose meter and biochemical analyzer. Methods Selected 4 sets of POCT glucose meter to detect the same sample and compared blood glucose results between Beckman CX-9 biochemical analyzer and POCT blood glucose meter. Results Measured results of Beckman CX-9 biochemical analyzer and POCT glucose meter were highly correlated(P>0.05), differences were not statistically significant. Conclusion POCT blood glucose meter is a consistent and convenient detection method for measuring blood glucose results as biochemical analyzer, so it is worthy of clinical promotion and application.%目的对比床旁检测(POCT)血糖仪与生化分析仪对血糖测定的结果,对POCT血糖仪的精密度与准确度进行探讨。方法选用4台POCT血糖仪对同一标本进行测定,并比较贝克曼CX-9生化分析仪与POCT血糖仪的测定结果。结果贝克曼CX-9生化分析仪与POCT血糖仪的测定结果高度相关(P>0.05),差异不具有统计学意义。结论 POCT血糖仪与生化分析仪对血糖的测定结果具有一致性,且方便快捷,可作为急诊的检测方法,值得在临床过程中推广、应用。

  1. Dielectric breakdown of cell membranes.

    Science.gov (United States)

    Zimmermann, U; Pilwat, G; Riemann, F

    1974-11-01

    With human and bovine red blood cells and Escherichia coli B, dielectric breakdown of cell membranes could be demonstrated using a Coulter Counter (AEG-Telefunken, Ulm, West Germany) with a hydrodynamic focusing orifice. In making measurements of the size distributions of red blood cells and bacteria versus increasing electric field strength and plotting the pulse heights versus the electric field strength, a sharp bend in the otherwise linear curve is observed due to the dielectric breakdown of the membranes. Solution of Laplace's equation for the electric field generated yields a value of about 1.6 V for the membrane potential at which dielectric breakdown occurs with modal volumes of red blood cells and bacteria. The same value is also calculated for red blood cells by applying the capacitor spring model of Crowley (1973. Biophys. J. 13:711). The corresponding electric field strength generated in the membrane at breakdown is of the order of 4 . 10(6) V/cm and, therefore, comparable with the breakdown voltages for bilayers of most oils. The critical detector voltage for breakdown depends on the volume of the cells. The volume-dependence predicted by Laplace theory with the assumption that the potential generated across the membrane is independent of volume, could be verified experimentally. Due to dielectric breakdown the red blood cells lose hemoglobin completely. This phenomenon was used to study dielectric breakdown of red blood cells in a homogeneous electric field between two flat platinum electrodes. The electric field was applied by discharging a high voltage storage capacitor via a spark gap. The calculated value of the membrane potential generated to produce dielectric breakdown in the homogeneous field is of the same order as found by means of the Coulter Counter. This indicates that mechanical rupture of the red blood cells by the hydrodynamic forces in the orifice of the Coulter Counter could also be excluded as a hemolysing mechanism. The detector

  2. Survival and activity of Streptococcus faecalis and Escherichia coli in tropical freshwater

    Energy Technology Data Exchange (ETDEWEB)

    Muniz, I.; Jimenez, L.; Toranzos, G.A.; Hazen, T.C. [Univ. of Puerto Rico, Rio Piedras (Puerto Rico)

    1988-12-31

    The survival of Streptococcus facecalis and Escherichia coli was studied in situ in a tropical rain forest watershed using membrane diffusion chambers. Densities were determined by acridine orange direct count and Coulter Counter. Population activity was determined by microautoradiography, cell respiration, and by nucleic acid composition. Densities of S. facecalis and E. coli decreased less than 1 log unit after 105 h as measured by direct count methods. Activity as measured by respiration, acridine orange activity, and microautoradiography indicated that both bacteria remained moderately active during the entire study. After 12 h, E. coli was more active than S. faecalis as measured by nucleic acid composition. E. coli and S. faecalis survived and remained active for more than 5 days. Consequently, both would seem to be unsuitable as indicators of recent fecal contamination in tropical waters.

  3. Crystalluria in marathon runners. 1. Standard marathon--males.

    Science.gov (United States)

    Irving, R A; Noakes, T D; Rodgers, A L; Swartz, L

    1986-01-01

    Epidemiological evidence suggests that marathon runners have a higher incidence of renal stone formation than occurs in the general population. Since crystalluria and stone disease are thought to be related, we subjected urine samples from a group of marathon runners to particle counting and sizing in a Coulter Counter equipped with a population accessory unit. The volume-size distribution curves so obtained were bimodal with one peak occurring in the 2-5 micron diameter range and a second in the 15-32 micron diameter range - a pattern that is remarkably similar to the distributions reported for recurrent idiopathic stone formers and distinctly different to those recorded for control subjects. Analyses by scanning electron microscopy and X-ray powder diffraction revealed other features which are regarded as typical of stone formers' crystalluria. These physicochemical data indicate that marathon runners may be at increased risk of urinary stone formation.

  4. The crystal growth of barium flouride in aqueous solution

    Science.gov (United States)

    Barone, J. P.; Svrjcek, D.; Nancollas, G. H.

    1983-06-01

    The kinetics of growth of barium flouride seed crystals were investigated in aqueous solution at 25°C using a constant composition method, in which the supersaturation and ionic strength were maintained constant by the addition of titrants consisting of barium nitrate and potassium flouride solutions. The rates of reaction, studied over a range of supersaturation (σ ≈ 0.4 to 1.0), were interpreted in terms of crystal growth models. A spiral growth mechanism best describes the data, and scanning electron microscopy indicates a three-dimensional growth. In the presence of inorganic additives such as phosphate, however, induction periods precede a morphological two-dimensional crystallization. Coulter Counter results show little crystal agglomeration.

  5. [Studies on the stability and some other important physical properties of polysaccharides polyphase liposome (PPL)].

    Science.gov (United States)

    Zou, Y Y; Su, D S; Han, G C; Gu, X Q

    1989-01-01

    The stability and some other important physical properties of PPL were investigated by means of electron microscopy and MPV-TAS experiments. The results are rated as follows: Good integrity of liposome homogeneity of its particle size were reached, and at 100 degrees C PPL was found to undergo sterilization without causing changes of its shape and encapsulation efficiency for 1 h. Also, the distribution of PPL particle size and the regularity of the change of PPL particle in size were determined by Coulter counter. Using this method, the kinetic equation of coalescence of polyphase liposome and the activation energy for coalescence were obtained, and through these results the stability of PPL is elucidated. PMID:2508432

  6. Visible and subvisible particles in the BCG immunotherapeutic product Immucyst®.

    Science.gov (United States)

    Kirkitadze, Marina; Remi, Elena; Bhandal, Kamajit; Carpick, Bruce

    2016-01-01

    Bacille Calmette-Guerin, BCG, is a live attenuated bovine tubercle bacillus used for the treatment of non-muscle invasive bladder cancer. In this study, an Electrical Sensing Zone (ESZ) method was developed to measure the particle count and the size of BCG immunotherapeutic (BCG IT), or ImmuCyst® product using a Coulter Counter Multisizer 4® instrument. The focus of this study was to establish a baseline for reconstituted lyophilized BCG IT product using visible and sub-visible particle concentration and size distribution as reportable values. ESZ method was used to assess manufacturing process consistency using 20 production scale lots of BCG IT product. The results demonstrated that ESZ can be used to accumulate product and process knowledge of BCG IT. PMID:27158432

  7. Ultra-sensitive flow measurement in individual nanopores through pressure--driven particle translocation.

    Science.gov (United States)

    Gadaleta, Alessandro; Biance, Anne-Laure; Siria, Alessandro; Bocquet, Lyderic

    2015-05-01

    A challenge for the development of nanofluidics is to develop new instrumentation tools, able to probe the extremely small mass transport across individual nanochannels. Such tools are a prerequisite for the fundamental exploration of the breakdown of continuum transport in nanometric confinement. In this letter, we propose a novel method for the measurement of the hydrodynamic permeability of nanometric pores, by diverting the classical technique of Coulter counting to characterize a pressure-driven flow across an individual nanopore. Both the analysis of the translocation rate, as well as the detailed statistics of the dwell time of nanoparticles flowing across a single nanopore, allow us to evaluate the permeability of the system. We reach a sensitivity for the water flow down to a few femtoliters per second, which is more than two orders of magnitude better than state-of-the-art alternative methods.

  8. Cell counting.

    Science.gov (United States)

    Phelan, M C; Lawler, G

    2001-05-01

    This unit presents protocols for counting cells using either a hemacytometer or electronically using a Coulter counter. Cell counting with a hemacytometer permits effective discrimination of live from dead cells using trypan blue exclusion. In addition, the procedure is less subject to errors arising from cell clumping or size heterogeneity. Counting cells is more quickly and easily performed using an electronic counter, but live-dead discrimination is unreliable. Cell populations containing large numbers of dead cells and/or cell clumps are difficult to count accurately. In addition, electronic counting requires resetting of the instrument for cell populations of different sizes; heterogeneous populations can give rise to inaccurate counts, and resting and activated cells may require counting at separate settings. In general, electronic cell counting is best performed on fresh peripheral blood cells. PMID:18770655

  9. Efficiency of dust sampling inlets in calm air.

    Science.gov (United States)

    Breslin, J A; Stein, R L

    1975-08-01

    Measurement of airborne dust concentrations usually involves drawing a sample of the dust-laden air into the measuring instrument through an inlet. Even if the surrounding air is calm, theoretical calculations predict that large particles may not be sampled accurately due to the combined effects of gravity and inertia on the particles near the sampling inlet. Tests were conducted to determine the conditions of particle size, inlet radius, and flow rare necessary for accurate dust sampling. A coal-dust aerosol was sampled simultaneously through inlets of different diameters at the same volume flow-rate and collected on filters. The dust was removed from the filters and the particles were counted and sized with a Coulter counter. Results showed that published criteria for inlet conditions for correct sampling are overly restrictive and that respirable-size particles are sampled correctly in the normal range or operation of most dust sampling instruments. PMID:1227283

  10. Dynamics of polymer nanoparticles through a single artificial nanopore with a high-aspect-ratio.

    Science.gov (United States)

    Cabello-Aguilar, Simon; Chaaya, Adib Abou; Bechelany, Mikhael; Pochat-Bohatier, Céline; Balanzat, Emmanuel; Janot, Jean-Marc; Miele, Philippe; Balme, Sébastien

    2014-11-14

    The development of nanometric Coulter counters for nanoparticle detection is an attractive and promising field of research. In this work, we have studied the influence of the nanopore surface state on charged polymer nanoparticle translocations. To make this, the translocation of carboxylate modified polystyrene microspheres (diameter 40, 70 and 100 nm) has been investigated through two kinds of high aspect ratio nanopores (negative and uncharged). The latter were tailored by a single track-etched and atomic layer deposition technique. It was shown that the mobility and the energy barrier are strongly dependent on nanopore surface charge. Typically if the latter exhibits negative surface charge, the microsphere mobility increases and the global energy barrier of entrance inside the nanopore decreases with its diameter, converse to the uncharged nanopore.

  11. Involving patients with leg ulcers in developing innovations in treatment and management strategies.

    Science.gov (United States)

    McNichol, Elaine

    2014-09-01

    Securing greater patient and public involvement is a central theme of health policy in many countries ( Coulter, 2011 ) and is a growing health-care phenomenon ( Staniszewska et al, 2011 ; World Health Organization, 2011 ). The challenge is how to ensure these voices are heard among the plethora of 'professional' voices representing different stakeholders of provider organisations, higher education, health-care industries and governments. Historically, in the management of leg ulcers, the professional 'user' voice has had a stronger input than that of the patient. This article outlines an approach that addresses this by moving beyond the concept of 'involving' the patient to putting the patient voice first, so that it sets the direction and leads the process in identifying innovation priorities in the management and treatment of chronic leg ulcers.

  12. El liderazgo docente y su relación con el estilo gerencial de los pasantes de Comunicación Social de la Universidad del Zulia

    Directory of Open Access Journals (Sweden)

    Rosario Fonseca

    2007-09-01

    Full Text Available Se propuso determinar la relación entre el liderazgo docente y el estilo gerencial de los estudiantes de Comunicación Social, mención Periodismo Impreso de LUZ, de acuerdo a la clasificación de Robbins, Stephen y Coulter (1999, y Guédez (1996 y siguiendo una metodología descriptiva-correlacional. Se aplicó un cuestionario para docentes y estudiantes de los últimos semestres. Los resultados demuestran que existe relación entre el liderazgo de los docentes, en su mayoría de tipo Democrático-Situacional, y el estilo gerencial de los estudiantes, predominó el estilo Estratégico y Situacional.

  13. Crop establishment of Silphium perfoliatum by precision seeding

    Directory of Open Access Journals (Sweden)

    Andreas Schäfer

    2015-12-01

    Full Text Available Silphium perfoliatum, a perennial composite from North America, has been found as a promising plant species during the search for alternative biogas substrates. Until now Silphium perfoliatum has been established by the time- and cost-intensive transplanting method. The precision sowing of Silphium perfoliatum reduces the process costs significantly. However, the bulky seed with mildness germination power complicates a well distributed and adequate plant establishment. In field tests a modified precision seeder was used for sowing Silphium perfoliatum which is primarily used for maize sowing.The hole diameter of the singling disc, the sowing coulter and the roller were modified. In the field tests the modified machinery proved an enhanced and uniform field emergence of Silphium perfoliatum.

  14. [Study on targeting drug delivery system--the characteristics of methotrexate microsphere and experimental treatment of hepatic tumor in rats by arterial embolization].

    Science.gov (United States)

    Chen, Q H; Lu, W G; Ge, Q H; Sheng, Q; Zhang, Y; Xie, X H; Wang, Y; Wu, M C; Zhang, X H

    1991-01-01

    Preparation of methotrexate microsphere (MTX-ms) by emulsion-freezing technique was introduced and the experimental results proved that MTX entrapped in the microspheres exhibited good stabilities towards temperature, cobalt-60 radiation and light. The dissolution and inflation rate of the microspheres in pH 7.4 buffer solution at different times measured by Coulter counter was presented. Antitumor activity of MTX-ms after hepatic arterial embolization was examined in a model of liver tumor in Wistar rats. The group of rats treated with MTX-ms showed a rather significant reduction in tumor growth and more extended tumor necrosis as compared with the other groups, e.g. normal saline solution, MTX solution, placebo gelatin-ms and the results demonstrate that the effect of arterial chemoembolization used by MTX-ms is superior to that of the groups either using arterial chemotherapy or arterial embolization alone in treating rat liver cancer.

  15. Grey scale enhancement of rabbit liver and kidney by intravenous injection of a new lipid-coated ultrasound contrast agent

    Institute of Scientific and Technical Information of China (English)

    Ping Liu; Yun-Hua Gao; Kai-Bin Tan; Zheng Liu; Song Zuo

    2004-01-01

    AIM: To assess the grey scale enhancement of a new lipidcoated ultrasound contrast agent in solid abdominal organs as liver and kidney.METHODS: Size distribution and concentration of the lipidcoated contrast microbubbles were analyzed by a Coulter counter. Two-dimensional (2D) second harmonic imaging of the hepatic parenchyma, the inferior vena cava and the right kidney of the rabbits were acquired before and after contrast agent injection. Images were further quantified by histogram in Adobe Photoshop 6.0. Time-intensity curves of hepatic parenchyma, inferior vena cava and renal cortex were generated from the original grey scale.RESULTS: The 2D images of hepatic parenchyma and cortex of the kidney were greatly enhanced after injection and the peak time could last more than 50 min.CONCLUSION: This new lipid ultrasound contrast agent could significantly enhance the grey scale imaging of the hepatic parenchyma and the renal cortex for more than 50 min.

  16. Ultra-Fast Low Concentration Detection of Candida Pathogens Utilizing High Resolution Micropore Chips

    Directory of Open Access Journals (Sweden)

    2009-03-01

    Full Text Available Although Candida species are the fourth most common cause of nosocomial blood stream infections in the United States, early diagnostic tools for invasive candidemia are lacking. Due to an increasing rate of candidemia, a new screening system is needed to detect the Candida species in a timely manner. Here we describe a novel method of detection using a solid-state micro-scale pore similar to the operational principles of a Coulter counter. With a steady electrolyte current flowing through the pore, measurements are taken of changes in the current corresponding to the shape of individual yeasts as they translocate or travel through the pore. The direct ultra-fast low concentration electrical addressing of C. albicans has established criteria for distinguishing individual yeast based on their structural properties, which may reduce the currently used methods’ complexity for both identification and quantification capabilities in mixed blood samples

  17. Red blood cell sorting with a multi-bed microfabricated filter

    International Nuclear Information System (INIS)

    A microfabricated fluidic chip for sorting red blood cells (RBCs) by size has been designed, fabricated and tested. The performance of the chip has been compared against a flow cytometer using samples from identical populations of cells, and statistically significant (p < 0.0005) differences in the measured cell size distributions were observed. The measurement paradigm reported here differs from previously demonstrated devices such as microfabricated Coulter counters or flow cytometers, in that the analysis is inherently parallel and is thus suitable for high throughput, point-of-care analysis. This study is empirical and semi-quantitative. However, important features of RBC trapping are characterized and indications for improved device design are described. (paper)

  18. Performance of calibration standards for antigen quantitation with flow cytometry.

    Science.gov (United States)

    Lenkei, R; Gratama, J W; Rothe, G; Schmitz, G; D'hautcourt, J L; Arekrans, A; Mandy, F; Marti, G

    1998-10-01

    In the frame of the activities initiated by the Task Force for Antigen Quantitation of the European Working Group on Clinical Cell Analysis (EWGCCA), an experiment was conducted to evaluate microbead standards used for quantitative flow cytometry (QFCM). An unified window of analysis (UWA) was established on three different instruments (EPICS XL [Coulter Corporation, Miami, FL], FACScan and FACS Calibur [Becton Dickinson, San Jose, CA]) with QC3 microbeads (FCSC, PR). By using this defined fluorescence intensity scale, the performance of several monoclonal antibodies directed to CD3, CD4, and CD8 (conjugated and unconjugated), from three manufacturers (BDIS, Coulter [Immunotech], and DAKO) was tested. In addition, the QIFI system (DAKO) and QuantiBRITE (BDIS), and a method of relative fluorescence intensity (RFI, method of Giorgi), were compared. mAbs reacting with three more antigens, CD16, CD19, and CD38 were tested on the FACScan instrument. Quantitation was carried out using a single batch of cryopreserved peripheral blood leukocytes, and all tests were performed as single color analyses. Significant correlations were observed between the antibody-binding capacity (ABC) values of the same CD antigen measured with various calibrators and with antibodies differing in respect to vendor, labeling and possible epitope recognition. Despite the significant correlations, the ABC values of most monoclonal antibodies differed by 20-40% when determined by the different fluorochrome conjugates and different calibrators. The results of this study indicate that, at the present stage of QFCM consistent ABC values may be attained between laboratories provided that a specific calibration system is used including specific calibrators, reagents, and protocols.

  19. Experimental determination of multiple thermodynamic and kinetic risk factors for nephrolithiasis in the urine of healthy controls and calcium oxalate stone formers: does a universal discriminator exist?

    Science.gov (United States)

    Rodgers, A L; Webber, D; Hibberd, B

    2015-11-01

    Nephrolithiasis is thought to be governed by urinary thermodynamic and kinetic risk factors. However, identification of one or more of these factors which consistently and unambiguously differentiates between healthy subjects (N) and calcium oxalate (CaOx) renal stone patients (SF) remains elusive. The present study addresses this challenge. 24 h urines were collected from 15 N and 10 SF. Urine compositions were used to compute thermodynamic risk indices including urinary ratios, quotients and supersaturation (SS) values, while CaOx metastable limits (MSL) were determined experimentally. Crystallisation kinetics was determined by measuring rates of particle formation (number, volume, size) using a Coulter counter multisizer (CC) and a Coulter flow cytometer (FC). Particle shapes were qualitatively differentiated by FC and were viewed directly by scanning electron microscopy. Several urinary composition ratios and risk quotients were significantly different between the groups. However, there were no significant differences between CaOx MSL or SS values. Using transformed FC data, the rate of CaOx crystallisation in SF was significantly greater than in N. This was not supported by CC measurements. There were no significant differences between the groups with respect to particle size or CaOx crystal growth rates. Single and aggregated CaOx dihydrate crystals were observed in both groups with equal frequency and there were no differences in the kinetic properties of these deposits. A few CaOx monohydrate crystals were observed in SF. Although several risk factors were found to be significantly different between the groups, none of them were consistently robust when compared to other cognate factors. Arguments were readily invoked which demonstrated inter-factor inconsistencies and conflicts. We suspect that a unique discriminatory factor, such as any of those which we investigated in the present study, may not exist. PMID:26198547

  20. 不同化学发光分析系统在皮质醇检测中的性能评价与比较%Performance evaluation and comparison of different chemiluminescence immunoassay systems in detection of cortisol

    Institute of Scientific and Technical Information of China (English)

    陈翊; 李颖能; 陈焕辉; 林涛; 汤勇才

    2016-01-01

    Objective To compare the analytical performance and results correlation of cortisol detection by the Beckman Access 2 Immunoassay System chemiluminescence system and the Roche COBAS E 411 electrochemical luminescence system .Methods The precision ,functional sensitivity ,reference range ,positive coincidence rate and negative coincidence rate of both Beckman Access 2 Immunoassay System and Roche COBAS E 411 systems for detecting cortisol were evaluated according to the method of ISO15198 .Results Both Beckman Access 2 Immunoassay System and Roche COBAS E 411 system exhibited better precision ,the functional sensitivity conformed to the lower limit of clinical detection set by the manufacturers ,after establishing the reference range of cortisol by the laboratory ,the cortisol values detected by these two systems were performed the linear regression analysis , the results indicated that the correlation between them was good ,the linear regression coefficient r2 =0 .977 ,and the positive and negative coincidence rates were 100% .Conclusion The two analytical systems have excellent performance for cortisol ,which all meet the clinical detection requirements .The methodological comparison shows good correlation between them ,and the detection re‐sults could be cited between these two systems by the regression equation .%目的:比较贝克曼Access 2 Immunoassay System化学发光免疫分析系统及罗氏COBAS E 411电化学发光免疫分析系统对皮质醇的分析性能与检测结果相关性。方法按照ISO15189中方法评估贝克曼及罗氏分析系统对皮质醇检测的精密度、功能灵敏度、参考范围、方法学、阳性符合率、阴性符合率。结果贝克曼与罗氏分析系统在皮质醇检测中精密度良好,功能灵敏度符合各自厂商设定的临床检测的下限,实验室自建参考范围后,将两分析系统对皮质醇的测定值进行线性回归分析,二者间相关性良好,线性回归系数 r2

  1. Comparability analysis between dry chemistry analyzer and wet chemistry analyzer%干化学与湿化学分析仪的可比性分析

    Institute of Scientific and Technical Information of China (English)

    李金密; 龚春梅; 何秀玲; 陈开春; 王丰

    2015-01-01

    目的:探讨不同生化分析仪16项常规生化指标测定结果的可比性。方法参照美国临床实验室标准化委员会(NCCLS)EP9‐A2文件,以贝克曼DXC800全自动生化分析仪为参比系统,以强生VITROS5600全自动生化免疫分析仪为待比系统,用患者新鲜血清分别在2台仪器上检测16项常规生化指标,检查离群点,计算相关系数和直线回归方程,评估2个系统的预期偏差,并以美国临床实验室改进修正案(CLIA′88)对室间评估的允许误差的1/2为判断依据,对强生VITROS5600干化学系统的临床可接受性进行评价。结果参比系统与待比系统具有良好的相关性,除丙氨酸氨基转移酶(ALT)、乳酸脱氢酶(LDH)、肌酐(Crea)及部份医学决定水平处天门冬氨酸氨基转移酶(AST)、总钙(Ca)、血清总蛋白(TP)测定结果的偏差外,其余指标测定结果的偏差临床均可接受。结论强生VITROS5600干化学分析仪与Beckman DXC800全自动生化分析仪的检测结果具有可比性,但ALT、AST、LDH、Ca、Crea、TP临床比对多水平处的偏差不可接受,仍有必要针对不同方法建立不同的参考区间。%Objective To investigate the comparability of the results of 16 routine biochemical indexes detected by different biochemical analyzers .Methods With the Beckman DXC800 automatic biochemical analyzer as the refer‐ence system and the VITROS5600 automatic biochemical immune analyzer as the compared system according to the EP9‐A2 ,guideline established by NCCLS .The 16 routine biochemical indexes were detected in serum of patients by the two instruments .The clinical acceptability of the VITROS5600 dry chemistry system was evaluated according to the judgment basis of 1/2 allowable error of the external quality assessment in CLIA′88 standard .Results The refer‐ence system and the compared system had better comparability .All tests showed good

  2. Terrestrial lichen response to partial cutting in lodgepole pine forests on caribou winter range in west-central British Columbia

    Directory of Open Access Journals (Sweden)

    Michaela J. Waterhouse

    2011-09-01

    Full Text Available In west-central British Columbia, terrestrial lichens located in older, lodgepole pine (Pinus contorta forests are important winter forage for woodland caribou (Rangifer tarandus caribou. Clearcut harvesting effectively removes winter forage habitat for decades, so management approaches based on partial cutting were designed to maintain continuous lichen-bearing habitat for caribou. This study tested a group selection system, based on removal of 33% of the forest every 80 years in small openings (15 m diameter, and two irregular shelterwood treatments (whole-tree and stem-only harvesting methods where 50% of the stand area is cut every 70 years in 20 to 30 m diameter openings. The abundance of common terrestrial lichens among the partial cutting and no-harvest treatments was compared across five replicate blocks, pre-harvest (1995 and post-harvest (1998, 2000 and 2004. The initial loss of preferred forage lichens (Cladonia, Cladina, Cetraria and Stereocaulon was similar among harvesting treatments, but there was greater reduction in these lichens in the openings than in the residual forest. After eight years, forage lichens in the group selection treatment recovered to pre-harvest amounts, while lichen in the shelterwood treatments steadily increased from 49 to 57% in 1998 to about 70% of pre-harvest amounts in 2004. Although not part of the randomized block design, there was substantially less lichen in three adjacent clearcut blocks than in the partial cuts. Regression analysis pre- and post-harvest indicated that increased cover of trees, shrubs, herbs, woody debris and logging slash corresponded with decreased forage lichen abundance. In the short-term, forestry activities that minimize inputs of woody debris, control herb and shrub development, and moderate the changes in light and temperatures associated with canopy removal will lessen the impact on lichen. Implementation of stand level prescriptions is only one aspect of caribou habitat

  3. Construction of an experimental plot seeder of wheat planting and compare it by imported one

    Directory of Open Access Journals (Sweden)

    I Eskandari

    2016-09-01

    Full Text Available Introduction Researchers frequently include multiple cultivars and fertility levels in field experiments. Therefore, the experiments sowing operation must represent a considerable saving in time and labor, compared to hand sowing. Greater flexibility in experimental design and setup could be achieved by equipment that enables quick changes in the cultivar and fertilizer rates from one plot to the next. A satisfactory seed drill must distribute a given quantity of seed evenly over a predetermined length of coulter row, the coulters must be spaced at exact intervals and depth of sowing must be uniform. In a self-propelled type of plot seeder, no coulter should run in a wheel track as the compaction of the soil can cause observable differences in vigor between plants in such a row and those in un-compacted rows. The machine should sow in succession from a try in which a series of seed pocket separated clearly and must be put into distributer funnel by an assistant operator. The length of gap being varied according to the nature and purpose of the plot. The objectives of this experiment were 1- to design and construct a local self-propelled plot seeder and 2- To compare it with the imported (Wintersteiger plot seeder in cereal breeding programs. Materials and Methods A small-plot seeder was designed and constructed to meet this objective. The unit consists of the following basic components: a toolbar for pulling a set of six blade coulter, an air compressor for lifting and putting down the openers and metering transmission drive wheel, an operators chair and work rack, one belt seed distribution. A cone-celled and rotor seed distributor is used for seed distribution to the openers. The cone system is connected to the gearbox and allows for great flexibility in changing cultivars, crop species, and plot length. This is driven by the separate drive wheel. The cone-celled distributor sows all the seed of the sample in making one complete turn. The

  4. Measurement of acute-phase protein of patients with HFRS%肾综合征出血热患者血清急性时相蛋白测定及其意义

    Institute of Scientific and Technical Information of China (English)

    郑昭敏; 皋群; 盖中涛; 吴世英

    2001-01-01

    为探讨急性时相蛋白(APP)与肾综合征出血热(HFRS)病情及预后的关系,采用Beckman Array 360特殊蛋白分析仪检测了253例HFRS患者(观察组)及30例正常人(对照组)空腹静脉血清APP[包括α1-抗胰蛋白酶(α1-AT)、α1-酸性糖蛋白(α1-AG)、触珠蛋白(HAP)、C反应蛋白(CRP)及转铁蛋白(Transferrin,TRF)]含量。结果:观察组急性期α1-AT、α1-AG、CRP含量明显升高,与对照组比较,P<0.05;TRF含量明显下降,与对照组比较,P<0.001。CRP及TRF的变化与病情轻重分别呈正负相关。认为急性时相蛋白含量变化可作为HFRS估计病情、判断预后和指导治疗的可靠指标。%To investigate the relation of acute-phase protein(APP) with haemorrhagic fever with renal syndrome(HFRS).Adopt the special protein analysor of Beckman array 360 inspect the contents of serum APP from 253 cases with HFRS(observes group) and normal controls[including α1-AT,α1-AG,haploglobin(HAP),CRP and TRF].The contents of α1-AT,α1-AG and CRP WERE Obviously increased but TRF decreased in acute phase.The result showed that the serum CRP level was directly correlated and TRF was inversely correlated to the patient′s condition.

  5. Influence of icterus on determination of serum inorganic phosphorous by phosphomolybdate method%黄疸对磷钼酸法测血清无机磷的影响

    Institute of Scientific and Technical Information of China (English)

    钟虹; 梁美春; 包国祥

    2013-01-01

    目的:探讨黄疸对磷钼酸法测血清无机磷的影响.方法:选择293例黄疸标本,根据《诊断学》标准,按黄疸程度分为轻度黄疸(含总胆红素34μmoL/L~ 170 μmol/L),中度黄疸(含总胆红素171μmoI/L~340 μmoL/L),重度黄疸(含总胆红素>340 μmoL/L),黄疸标本分别在雅培AEROSET和贝克曼LX20两台生化仪上分别进行血清无机磷测定,所测结果进行统计学分析.结果:不同程度的黄疸标本在两台全自动生化仪上所测结果比较均有明显差别(P均为0,P<0.01),且均有良好的相关性.结论:黄疸标本在雅培组影响血清无机磷的测定,所测结果低于贝克曼组,并随黄疸程度的加深,测定结果偏低的趋势有加大的倾向.%Objective:To evaluate the influence of icterus on the determination of serum inorganic phosphorous by phosphomolybdate method. Methods: Two hundred and ninety - three patients were classified into mild icterus (Total bilirubin 34 μmoL/L ~ 170 μmoL/L), medium icterus(Total bilirubin 171 μmoL/L ~340 μmoL/L), serious icterus (Total bilirubin > 340 μmoL/L) according to the "Diagnostics". Two kinds of automatic biochemistry analyzers (Abbott Aeroset and Beckman LX20) were employed to measure the serum inorganic phosphorous in different degrees icterus. All results were analyzed statistically. Results: The measured values obtained by the two kinds of automatic biochemistry analyzers had significant difference. And they have good correlation. Conclusion.- With Abbott Aeroset, the measured values of the serum inorganic phosphorous are influenced by the icterus. The measured values are below those by Beckman. And with the deepening of icterus degree, the measured values showed a decrease trend.

  6. Automated haematology analysis to diagnose malaria

    Directory of Open Access Journals (Sweden)

    Grobusch Martin P

    2010-11-01

    Full Text Available Abstract For more than a decade, flow cytometry-based automated haematology analysers have been studied for malaria diagnosis. Although current haematology analysers are not specifically designed to detect malaria-related abnormalities, most studies have found sensitivities that comply with WHO malaria-diagnostic guidelines, i.e. ≥ 95% in samples with > 100 parasites/μl. Establishing a correct and early malaria diagnosis is a prerequisite for an adequate treatment and to minimizing adverse outcomes. Expert light microscopy remains the 'gold standard' for malaria diagnosis in most clinical settings. However, it requires an explicit request from clinicians and has variable accuracy. Malaria diagnosis with flow cytometry-based haematology analysers could become an important adjuvant diagnostic tool in the routine laboratory work-up of febrile patients in or returning from malaria-endemic regions. Haematology analysers so far studied for malaria diagnosis are the Cell-Dyn®, Coulter® GEN·S and LH 750, and the Sysmex XE-2100® analysers. For Cell-Dyn analysers, abnormal depolarization events mainly in the lobularity/granularity and other scatter-plots, and various reticulocyte abnormalities have shown overall sensitivities and specificities of 49% to 97% and 61% to 100%, respectively. For the Coulter analysers, a 'malaria factor' using the monocyte and lymphocyte size standard deviations obtained by impedance detection has shown overall sensitivities and specificities of 82% to 98% and 72% to 94%, respectively. For the XE-2100, abnormal patterns in the DIFF, WBC/BASO, and RET-EXT scatter-plots, and pseudoeosinophilia and other abnormal haematological variables have been described, and multivariate diagnostic models have been designed with overall sensitivities and specificities of 86% to 97% and 81% to 98%, respectively. The accuracy for malaria diagnosis may vary according to species, parasite load, immunity and clinical context where the

  7. Crystallisation properties in stone forming and normal subjects' urine diluted using a standardised procedure to match the composition of urine in the distal part of the distal tubule and the middle part of the collecting duct.

    Science.gov (United States)

    Tiselius, H G; Hallin, A; Lindbäck, B

    2001-04-01

    Using a standardised procedure, we assessed the crystallisation properties of calcium phosphate in urine with a composition matching that in the distal part of the distal tubules (DTd) and of calcium oxalate in urine with a composition matching that in the mid-collecting duct (CDm). We used 8-h urine samples collected between 2200 h and 0600 h with sodium azide as preservative. Urine from ten patients with recurrent CaOx stone formation and from ten normal subjects was used for the measurements. The DTd and CDm samples were obtained by diluting the voided 8-h urine to 3000 ml and 1750 ml per 1.73 m2 body surface area, respectively. The nucleation was studied in DTd urine following supersaturation with CaP. The crystal size distribution was assessed with a Coulter counter both following supersaturation of DTd urine with CaP and of CDm urine with CaOx. The crystallisation of CaP in DTd urine as well as that of CaOx in CDm urine, in the presence of CaP crystals that had been precipitated in DTd urine, was measured with the isotope technique. The inhibition of CaOx and brushite crystal aggregation in standardised diluted aliquots of DTd and CDm urine was assessed spectrophotometrically as the rate of sedimentation. There was a slightly increased sedimentation rate and a lower initial absorbance in DTd urine from stone formers supersaturated with CaP. Although these findings might reflect a state of increased crystal aggregation in stone formers' urine, this could not be confirmed by crystal size measurements in the Coulter counter. The inhibition of brushite crystal aggregation in DTd urine was significantly in stone formers' urine than in normal subjects' urine (P crystallisation assessments was carried out on a small number of standardised diluted urine samples only, the results nevertheless emphasise a defect in aggregation inhibition as one important determinant for an abnormal calcium salt crystallisation in patients with recurrent stone formations. This

  8. Mecanismos de abertura do sulco e adubação nitrogenada no cultivo do feijoeiro em sistema plantio direto Furrows opening mechanism for nitrogen fertilizer application in common bean crop under no-tillage

    Directory of Open Access Journals (Sweden)

    Orivaldo Arf

    2008-01-01

    Full Text Available Algumas culturas têm pouca adaptação ao sistema plantio direto, em vista da maior compactação da camada superficial do solo e, nesse caso, o mecanismo utilizado na semeadora para a abertura dos sulcos para deposição do fertilizante pode ter grande importância no sentido de facilitar a penetração das raízes. Este experimento foi desenvolvido em Selvíria (MS, com o objetivo de avaliar a produtividade do feijão de inverno cultivado em sistema plantio direto, em função da utilização de mecanismos de abertura para distribuição de fertilizantes na semeadura e da adubação nitrogenada em cobertura. O delineamento experimental utilizado foi em blocos casualizados, utilizando-se esquema fatorial 2 x 6, constituído por mecanismos de distribuição de fertilizante (haste escarificadora e disco duplo e doses de N em cobertura (0, 25, 50, 75, 100 e 125 kg ha-1, com quatro repetições. Recomenda-se o uso da haste escarificadora como mecanismo de distribuição do fertilizante, para o cultivo do feijoeiro de inverno. A adubação nitrogenada em cobertura proporciona incrementos à produtividade do feijoeiro de inverno.Some crops have shown no adaptation to no-tillage system as a function of compaction soil superficial layer. In way, the mechanism used in seeder to open furrows for deposition of fertilizer can have great importance to facilitate the penetration of roots. This experiment was carried in Selvíria (MS, with the objective to evaluate the winter common bean crop yield under no-tillage system, as function of fertilizer distribution opening mechanisms in sowing (chisel and coulter blade and sidedressing nitrogen application (0, 25, 50, 75, 100 and 125 kg ha-1. The experimental design was a randomized block, arranged in a 2 x 6 factorial scheme, constituted by fertilizer distribution opening mechanisms in sowing (chisel and coulter blade and sidedressing nitrogen doses (0, 25, 50, 75, 100 and 125 kg ha-1, with four replications

  9. C-reactive protein as a risk factor for acute coronary syndrome

    Institute of Scientific and Technical Information of China (English)

    杨胜利; 何秉贤; 何作云; 张华; 何学兰; 张伟

    2002-01-01

    Objective We assessed thelevels of C-reactive protein ( CRP ) in patients with acute coronary syndrome (ACS) [including unstable angina pectoris (UAP), acute myocardial infarction (AMI) and sudden cardiac death (SCD) ] compared with non-ACS [including stable angina pectoris (SAP), old myocardial infarction (OMI) and healthy volunteers] and sought to test whether CRP are associated with clinical acute coronary syndrome. Methods Ultrasensitive immunoassay (rate nephelometry with the Beckman Array multitest immunoassay system) wasused to measure CRP levels in 91 patients with ACS(20 UAP, 71 AMI including 2 SCD) and non-ACS (34SAP, 25 patients with healing phase of AMI, 41 OMI and 94 control healthy subjects) Results CRP levels were higher in ACS group (18.50 + 23.98 mg/L [ SE 2.51, n = 91 ] ) compared with non - ACS group (3.89+7.14mg/L[SE0.51, n=194]) (P <0.01).Using Logistic Regression, CRP was a potent determinant of ACS ( OR = 1.65) Conclusion These results suggest that CRP has a strong association with ACS, and CRP is a risk factor of ACS.

  10. Smoking cessation: an application of theory of planned behavior to understanding progress through stages of change.

    Science.gov (United States)

    Bledsoe, Linda K

    2006-07-01

    The purpose of this research was to investigate variables relevant to smoking cessation early in the process of change through an application of the Theory of Planned Behavior [Ajzen, I. (1985). From intentions to actions: A theory of planned behavior. In J. Kuhl and J. Beckman (Eds). Action-control: From cognition to behavior (pp.11-39). Heidelberg: Springer.] to the temporal structure provided by the Transtheoretical Model. Study 1 was a preliminary elicitation study (n=68) conducted to ground the concepts used in the model testing in Study 2 [Ajzen, I., Fishbein, M. (1980). Understanding attitudes and predicting social behavior, Englewood Cliffs, NJ: Prentice-Hall.]. Study 2 tested the proposed model fit with data from a sample of 230 adult smokers. Structural equation modeling did not support the Theory of Planned Behavior as a model of motivation for progress through the stages of change and highlighted measurement issues with perceived behavioral control. A modified model using the Theory of Reasoned Action provided a good fit to the data, accounting for approximately 64% of the variance in intention to quit smoking and stage of change. This research addresses the need for a more complete theoretical rationale for progress through stages of change. PMID:16182458

  11. Smoking cessation: an application of theory of planned behavior to understanding progress through stages of change.

    Science.gov (United States)

    Bledsoe, Linda K

    2006-07-01

    The purpose of this research was to investigate variables relevant to smoking cessation early in the process of change through an application of the Theory of Planned Behavior [Ajzen, I. (1985). From intentions to actions: A theory of planned behavior. In J. Kuhl and J. Beckman (Eds). Action-control: From cognition to behavior (pp.11-39). Heidelberg: Springer.] to the temporal structure provided by the Transtheoretical Model. Study 1 was a preliminary elicitation study (n=68) conducted to ground the concepts used in the model testing in Study 2 [Ajzen, I., Fishbein, M. (1980). Understanding attitudes and predicting social behavior, Englewood Cliffs, NJ: Prentice-Hall.]. Study 2 tested the proposed model fit with data from a sample of 230 adult smokers. Structural equation modeling did not support the Theory of Planned Behavior as a model of motivation for progress through the stages of change and highlighted measurement issues with perceived behavioral control. A modified model using the Theory of Reasoned Action provided a good fit to the data, accounting for approximately 64% of the variance in intention to quit smoking and stage of change. This research addresses the need for a more complete theoretical rationale for progress through stages of change.

  12. Assay-specific artificial neural networks for five different PSA assays and populations with PSA 2-10 ng/ml in 4,480 men.

    Science.gov (United States)

    Stephan, Carsten; Xu, Chuanliang; Cammann, Henning; Graefen, Markus; Haese, Alexander; Huland, Hartwig; Semjonow, Axel; Diamandis, Eleftherios P; Remzi, Mesut; Djavan, Bob; Wildhagen, Mark F; Blijenberg, Bert G; Finne, Patrik; Stenman, Ulf-Hakan; Jung, Klaus; Meyer, Hellmuth-Alexander

    2007-03-01

    Use of percent free PSA (%fPSA) and artificial neural networks (ANNs) can eliminate unnecessary prostate biopsies. In a total of 4,480 patients from five centers with PSA concentrations in the range of 2-10 ng/ml an IMMULITE PSA-based ANN (iANN) was compared with other PSA assay-adapted ANNs (nANNs) to investigate the impact of different PSA assays. ANN data were generated with PSA, fPSA (assays from Abbott, Beckman, DPC, Roche or Wallac), age, prostate volume, and DRE status. In 15 different ROC analyses, the area under the curve (AUC) in the PSA ranges 2-4, 2-10, and 4-10 ng/ml for the nANN was always significantly larger than the AUC for %fPSA or PSA. The nANN and logistic regression models mostly also performed better than the iANN. Therefore, for each patient population, PSA assay-specific ANNs should be used to optimize the ANN outcome in order to reduce the number of unnecessary biopsies. PMID:17333205

  13. Roger Hayward and the Invention of the Two-Mirror Schmidt

    Science.gov (United States)

    Bell, T. E.

    2005-12-01

    Roger Hayward (1899-1979), now virtually unknown, was a multitalented architect, scientific illustrator, and optical inventor. Remembered primarily for illustrating Scientific American magazine's Amateur Scientist column between 1949 and 1974, he also illustrated more than a dozen textbooks in optics, physics, geology, oceanography, and chemistry, several of which became classics in their fields. He designed façades with astronomical themes for major buildings in Los Angeles, California, and sculpted mammoth, realistic models of the moon for Griffith Observatory, Adler Planetarium, and Disneyland. Throughout his life, he recreationally painted watercolors and oils that at least one critic likened to the work of John Singer Sargent. Hayward is least known as an optical designer, yet he made significant contributions to the DU spectrophotometer that established the multimillion-dollar company Beckman Instruments. During the pre-radar days of World War II at Mount Wilson Observatory, Hayward invented a classified Cassegrain version of the Schmidt telescope especially adapted for nighttime infrared aerial photography, plus extraordinarily simple machines that allowed inexperienced soldiers to grind, polish, and test accurate aspheric Schmidt correcting plates at speeds compatible with mass production - and later received U.S. patents for them all. This paper, drawn in part from unpublished letters between Hayward and Albert G. Ingalls, will feature little-known images of Hayward's work.

  14. Comparison and bias analysis of BUN, Cr and UA between two different biochemical analysis systems%血尿素氮、肌酐及尿酸在两种生化分析系统的比对和偏倚评估

    Institute of Scientific and Technical Information of China (English)

    吕慧; 赖战峰; 李海炜; 林丽文; 秦雪; 李山

    2012-01-01

    Objective To discuss the comparability of blood urea nitrogen (BUN),creatinine (Cr),uric acid (UA) results and provide basis for result concordance in different biochemical systems.Methods The within-run precision and the between-day precision for the median and high values of quality control materials were tested in Beckman LX-20 and Hitachi 7600 chemistry analyzer respectively,and the evaluation criteria were according to the precision requirement of manufacturer and our laboratory.According to American Clinical and Laboratory Standards Institute (CLSI) document EP9-A2,serum BUN,Cr,UA from 40 patients were detected by Hitachi 7600 chemistry analyzer as experimental method and by Beckman LX-20 chemistry analyzer as comparative method.The relative bias ( SE%) between experimental method and comparative method was calculated and the concordance of biochemical analysis system was determined according to EQA requirements and acceptable performance criteria Results The between-run and between-day precision coefficients of variation for BUN,Cr and UA in two biochemical analysis systems were lower than the precision requirement of manufacturer and our laboratory.In Hitachi 7600 chemistry analyzer,the SE% for BUN at three levels of medical decision were 9.4%,2.0% and 0.8%,the SE% for Cr were 7.3%,3.8% and 2.8%,the SE% for UA were 7.9%,1.7% and 1.2%.Two biochemical analysis systems were not comparable in BUN analysis because SE% of serum BUN levels detected by Hitachi 7600 at low medical decision level exceeded the specified range.The remaining items between two biochemical analysis systems were concordant.Conclusion While the same test is detected by more than two systems in one laboratory,it is necessary to do method comparison and bias evaluation for concordance in order to ensure the comparability among test results.%目的 探讨不同的生化检测系统间血尿素氮(BUN)、肌酐(Cr)、尿酸(UA)三项指标测定结果的可比性,

  15. Cytotoxic Effect of Erythroxylum suberosum Combined with Radiotherapy in Head and Neck Cancer Cell Lines.

    Science.gov (United States)

    Macedo, Taysa B C; Elias, Silvia T; Torres, Hianne M; Yamamoto-Silva, Fernanda Paula; Silveira, Dâmaris; Magalhães, Pérola O; Lofrano-Porto, Adriana; Guerra, Eliete N S; Silva, Maria Alves G

    2016-01-01

    The mouth and oropharynx cancer is the 6th most common type of cancer in the world. The treatment may involve surgery, chemotherapy and radiotherapy. More than 50% of drugs against cancer were isolated from natural sources, such as Catharanthus roseus and epipodophyllotoxin, isolated from Podophyllum. The biggest challenge is to maximize the control of the disease, while minimizing morbidity and toxicity to the surrounding normal tissues. The Erythroxylum suberosum is a common plant in the Brazilian Cerrado biome and is popularly known as "cabelo-de-negro". The objective of this study was to evaluate the cytotoxic activity of Erythroxylum suberosum plant extracts of the Brazilian Cerrado biome associated with radiotherapy in human cell lines of oral and hypopharynx carcinomas. Cells were treated with aqueous, ethanolic and hexanic extracts of Erythroxylum suberosum and irradiated at 4 Gy, 6 Gy and 8 Gy. Cytotoxicity was evaluated by MTT assay and the absorbance was measured at 570 nm in a Beckman Counter reader. Cisplatin, standard chemotherapy, was used as positive control. The use of Erythroxylum suberosum extracts showed a possible radiosensitizing effect in vitro for head and neck cancer. The cytotoxicity effect in the cell lines was not selective and it is very similar to the effect of standard chemotherapy. The aqueous extract of Erythroxylum suberosum, combined with radiotherapy was the most cytotoxic extract to oral and hypopharynx carcinomas. PMID:27007356

  16. Effect of training on the performance of blood glucose monitoring using a reagent strip (Glucoprofil).

    Science.gov (United States)

    Chantelau, E; Starostina, E G; Antsiferov, M B

    1990-11-01

    A relatively simple, new test-strip for blood-glucose monitoring, the Glucoprofil strip with a reaction-zone of film-foil, was evaluated by testing 100 different blood glucose concentrations between 2.1 and 29.7 mmol/l. The results were obtained visually before and after training to read the color changes of the strip according to the scale on the strip-container. Plasma glucose measurements with the Beckman Glucose-Analyzer were used for reference. The results show that the Glucoprofil-strip readings correlated well with the reference method (r = 0.97), thorough training provided. The mean deviation of the strip results was less than 1 mmol/l. Similar results were obtained using another blood glucose strip, the Haemoglukotest 20-800 R. Our study indicates that the performance of the Glucoprofil strip is satisfactory, and hence the strip may be useful for clinical purposes. Consistent with previous reports we could demonstrate that training improved the reading results of the Glucoprofil strip.

  17. 对甲氧基苯磺酰氯柱前衍生生物胺的高效液相色谱分离%Separation of biogenic amines by high performance liquid chromatography with 4-methoxy benzenesulphonyl chloride as a precolumn derivatization reagent

    Institute of Scientific and Technical Information of China (English)

    屠春燕; 袁艳娟; 吴燕娇; 唐美华; 韦萍; 李寿椿

    2010-01-01

    采用对甲氧基苯磺酰氯作为柱前衍生试剂,RP-HPLC为分析模式,建立了一种新的生物胺衍生化方法,并对葡萄酒中生物胺含量进行检测. 通过液质联用对产物进行定性,研究并确定了最适衍生化条件:衍生温度50 ℃,缓冲液pH 9.0,衍生时间15 min. 实验建立了7种生物胺的HPLC分离方法:BeckmanODS柱;流动相A为10 mmol/L的NH_4Ac溶液(pH 6.37),B相为乙腈;采用梯度洗脱;流速1 mL/min;检测波长240 nm, 室温.

  18. Cytotoxic effect of Erythroxylum suberosum combined with radiotherapy in head and neck cancer cell lines

    International Nuclear Information System (INIS)

    The mouth and oropharynx cancer is the 6th most common type of cancer in the world. The treatment may involve surgery, chemotherapy and radiotherapy. More than 50% of drugs against cancer were isolated from natural sources, such as Catharanthus roseus and epipodophyllotoxin, isolated from Podophyllum. The biggest challenge is to maximize the control of the disease, while minimizing morbidity and toxicity to the surrounding normal tissues. The Erythroxylum suberosum is a common plant in the Brazilian Cerrado biome and is popularly known as 'cabelo-de-negro'. The objective of this study was to evaluate the cytotoxic activity of Erythroxylum suberosum plant extracts of the Brazilian Cerrado biome associated with radiotherapy in human cell lines of oral and hypopharynx carcinomas. Cells were treated with aqueous, ethanolic and hexanic extracts of Erythroxylum suberosum and irradiated at 4 Gy, 6 Gy and 8 Gy. Cytotoxicity was evaluated by MTT assay and the absorbance was measured at 570 nm in a Beckman Counter reader. Cisplatin, standard chemotherapy, was used as positive control. The use of Erythroxylum suberosum extracts showed a possible radiosensitizing effect in vitro for head and neck cancer. The cytotoxicity effect in the cell lines was not selective and it is very similar to the effect of standard chemotherapy. The aqueous extract of Erythroxylum suberosum, combined with radiotherapy was the most cytotoxic extract to oral and hypopharynx carcinomas. (author)

  19. The determination of calcium in phosphate, carbonate, and silicate rocks by flame photometer

    Science.gov (United States)

    Kramer, Henry

    1956-01-01

    A method has been developed for the determination of calcium in phosphate, carbonate, and silicate rocks using the Beckman flame photometer, with photomultiplier attachement. The sample is dissolved in hydrofluoric, nitric, and perchloric acids, the hydrofluoric and nitric acids are expelled, a radiation buffer consisting of aluminum, magnesium, iron, sodium, potassium, phosphoric acid, and nitric acid is added, and the solution is atomized in an oxy-hydrogen flame with an instrument setting of 554 mµ. Measurements are made by comparison against calcium standards, prepared in the same manner, in the 0 to 50 ppm range. The suppression of calcium emission by aluminum and phosphate was overcome by the addition of a large excess of magnesium. This addition almost completely restores the standard curve obtained from a solution of calcium nitrate. Interference was noted when the iron concentration in the aspirated solution (including the iron from the buffer) exceeded 100 ppm iron. Other common rock-forming elements did not interfere. The results obtained by this procedure are within ± 2 percent of the calcium oxide values obtained by other methods in the range 1 to 95 percent calcium oxide. In the 0 to 1 percent calcium oxide range the method compares favorably with standard methods.

  20. Amino acid composition of some Mexican foods.

    Science.gov (United States)

    Morales de León, Josefina; Camacho, M Elena; Bourges, Héctor

    2005-06-01

    Knowledge of the amino acid composition of foods is essential to calculate their chemical score, which is used to predict protein quality of foods and diets. Though amino acid composition of many foods is reasonably well established, better knowledge is needed on native foods consumed in different regions and countries. This paper presents the amino acid composition of different presentations of raw and processed foods produced and consumed in Mexico. The amino acid composition was determined using Beckman amino acid analyzers (models 116 and 6300). Tryptophan was determined using the Spies and Chambers method. Of the different foods analyzed, some comments are made on native or basic foods in Mexico: Spirulin, where lysine is the limiting amino acid, with a chemical score of 67%, is a good source of tryptophan (1.16g/16 gN); amaranth contains high levels of sulphur amino acids (4.09 to 5.34 g/16gN), with a protein content of 15 g/100g; and pulque, a Pre-Hispanic beverage that contains high levels of tryptophan (2.58 g/16 gN) and sulphur amino acids (2.72 g/16 gN). Finally, insects are good sources of sulphur amino acids and lysine.

  1. The value of Golgi protein 73 detection combined with alpha-fetoprotein,α-L-fucosidase,α-antitrypsin in the diagnosis of primary liver cancer%高尔基体蛋白73联合甲胎蛋白、α-L 岩藻糖苷酶、α-抗胰蛋白酶检测对原发性肝癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    黄华; 梁红梅; 胥慧; 周庭雄; 王建蔚

    2015-01-01

    Objective To study the diagnostic value of the golgi protein 73 (GP73) combined with alpha‐fetoprotein (AFP) ,α‐L‐fucosidase (AFU) andα‐antitrypsin(α‐AT) detection in primary hepatic carcinoma(PHC) .Methods 100 patients with PHC ,100 patients with hepatitis and cirrhosis ,and 100 healthy people hospitalized in the hospital from October 2011 to May 2013 were en‐rolled in the study .Serum AFP levels were detected by using Beckman DxI800 chemiluminescent analyzer ,serum AFU ,α‐AT levels were detected by using Beckman DxC800 automatic biochemical analyzer ,and GP73 levels were measured by using enzyme‐linked immunosorbent GP73 quantitative detection kits .Results In patients with PHC and patients with hepatitis and cirrhosis ,serum GP73 ,AFP ,AFU ,α‐AT levels were significantly higher than healthy people(P<0 .05) .In patients with PHC ,GP73 ,AFP ,AFU ,α‐AT levels were significantly higher than those in patients with hepatitis and cirrhosis(P<0 .05) .The sensitivities of single serum GP73 ,AFP ,AFU ,α‐AT detection were 75% ,72% ,23% ,67% respectively and specificity were 95% ,95% ,100% ,97% respective‐ly .The sensitivity and specificity of the 4 tumor markers′combined detection were 99% and 100% .The sensitivity of combined de‐tection was significantly different from single detection(P<0 .05) .Conclusion GP73 ,AFP ,AFU ,α‐AT may improve the diagnostic efficiency of primary liver cancer .%目的:研究高尔基体蛋白73(GP73)联合甲胎蛋白(AFP)、α‐L岩藻糖苷酶(AFU)、α‐抗胰蛋白酶(α‐AT)检测对原发性肝癌诊断的价值。方法选取2011年10月至2013年5月在该院住院及就诊的原发性肝癌、肝炎和肝硬化患者及健康体检者各100例作为研究对象,使用Beckman DxI800化学发光分析仪检测血清AFP水平;用Beckman DxC800全自动生化分析仪检测血清AFU、α‐AT水平;用GP73酶联免疫定量检测试剂盒测定 GP73水平。结果原发性

  2. Evaluation of Thermal Control Coatings and Polymeric Materials Exposed to Ground Simulated Atomic Oxygen and Vacuum Ultraviolet Radiation

    Science.gov (United States)

    Kamenetzky, R. R.; Vaughn, J. A.; Finckenor, M. M.; Linton, R. C.

    1995-01-01

    Numerous thermal control and polymeric samples with potential International Space Station applications were evaluated for atomic oxygen and vacuum ultraviolet radiation effects in the Princeton Plasma Physics Laboratory 5 eV Neutral Atomic Oxygen Facility and in the MSFC Atomic Oxygen Drift Tube System. Included in this study were samples of various anodized aluminum samples, ceramic paints, polymeric materials, and beta cloth, a Teflon-impregnated fiberglass cloth. Aluminum anodizations tested were black duranodic, chromic acid anodize, and sulfuric acid anodize. Paint samples consisted of an inorganic glassy black paint and Z-93 white paint made with the original PS7 binder and the new K2130 binder. Polymeric samples evaluated included bulk Halar, bulk PEEK, and silverized FEP Teflon. Aluminized and nonaluminized Chemfab 250 beta cloth were also exposed. Samples were evaluated for changes in mass, thickness, solar absorptance, and infrared emittance. In addition to material effects, an investigation was made comparing diffuse reflectance/solar absorptance measurements made using a Beckman DK2 spectroreflectometer and like measurements made using an AZ Technology-developed laboratory portable spectroreflectometer.

  3. 固相萃取-反相高效液相色谱法同时测定人血清中地西泮及氯硝西泮%Simultaneous determination of diazepam and clonazepam in human serum by solid phase extraction RP-HPLC

    Institute of Scientific and Technical Information of China (English)

    张吟; 陈一农; 郑兴中

    2002-01-01

    目的:建立一种快速、灵敏的同时测定人血清中地西泮与氯硝西泮的HPLC方法.方法:血样经C18-SPE小柱萃取后,在Beckman ODS色谱柱上,以甲醇-水(60∶40)为流动相,流速1.0 ml*min-1,231 nm检测.结果:氯硝西泮、地西泮的保留时间分别为4.5,7.4 min;线性范围分别为0.03~1.00,0.25~10.00 μg*ml-1;平均日内RSD分别为2.40%,2.30%;平均日间RSD分别为2.62%,2.49%;平均回收率分别为99.7%,99.5%,平均RSD分别为3.28%,3.36%.结论:本方法可用于同时监测地西泮与氯硝西泮血药浓度.

  4. Axon clinical chemistry analyzer evaluated according to ECCLS protocol.

    Science.gov (United States)

    Brenna, S; Prencipe, L

    1992-10-01

    We assessed the analytical performance of the Axon system (Bayer Diagnostici), according to the European Committee for Clinical Laboratory Standards guidelines, for assay of 12 analytes: cholesterol, creatinine, glucose, total protein, urea, uric acid, alkaline phosphatase, alpha-amylase, aspartate aminotransferase, creatine kinase, sodium, and potassium. The field evaluation lasted approximately 5 months and involved the collection of approximately 10,000 data points with the Axon. The following results were obtained: The highest CVs for controls and human sera at different concentration/activity values were 2.2% for within-run imprecision (n = 60; 3 days, pooled estimate) and 3.5% for the between-day imprecision (n = 20 days). Close correlation was found with results for patients' specimens assayed with comparative instruments (Hitachi 717 for substrates and enzymes, Beckman Synchron EL/E4A for electrolytes). No drift was observed during 8 h of operation. The linearity range was broad, sometimes exceeding the manufacturer's claims. No sample-, reagent-, or cuvette-related carryover was found. Measurement of control sera gave results within +/- 5% of the assigned values. We conclude that good reliability and practicability make the Axon system suitable for laboratories with various needs.

  5. Biological characterization of a novel and potent gonadotropin releasing hormone (GnRH) antagonist LXT-101

    Institute of Scientific and Technical Information of China (English)

    Xiao-liCHI; Wen-xiaZHOU; Jun-pingCHENG; Yong-xiangZHANG; Ke-liangLIU

    2005-01-01

    AIM The biological characterization of LXT-101 was investigated in vivo using intact male rats and nude mice bearing xenografts of LNCaP prostate cancer. The effect of LXT-101 on the proliferation of androgen -sensitive prostate cancer cell LNCaP and androgen-insensitive DU145 and PC-3M in vitro was also determined. METHODS Rats were injected subcutaneously with LXT-101 while control animals received only vehicle (5% mannitol). Blood samples were collected at different time after adminis-tration of LXT-101. The androgen-dependent LNCaP prostate cancer cells were grown, mixed with Matrigel and injected subcutaneously into nude mice. Experimental group received LXT-101 injectionfor up to 4 weeks. At the end point, blood samples were drawn and the excised tumors and sex organswere weighted. The serum testosterone was determined by specific immunochemiluminescence assay using kits produced by Beckman-Counter Co. The mRNA expressions of the genes of hormone receptor related to the gonadal axis were investigated by real-time RT-PCR technique. Cell viability was determined by MTT method.

  6. Angiotensin-converting enzyme inhibition studies by natural leech inhibitors by capillary electrophoresis and competition assay.

    Science.gov (United States)

    Deloffre, Laurence; Sautiere, Pierre-Eric; Huybrechts, Roger; Hens, Korneel; Vieau, Didier; Salzet, Michel

    2004-06-01

    A protocol to follow the processing of angiotensin I into angiotensin II by rabbit angiotensin-converting enzyme (ACE) and its inhibition by a novel natural antagonist, the leech osmoregulator factor (LORF) using capillary zonal electrophoresis is described. The experiment was carried out using the Beckman PACE system and steps were taken to determine (a) the migration profiles of angiotensin and its yielded peptides, (b) the minimal amount of angiotensin II detected, (c) the use of different electrolytes and (d) the concentration of inhibitor. We demonstrated that LORF (IPEPYVWD), a neuropeptide previously found in leech brain, is able to inhibit rabbit ACE with an IC(50) of 19.8 micro m. Interestingly, its cleavage product, IPEP exhibits an IC(50) of 11.5 micro m. A competition assay using p-benzoylglycylglycylglycine and insect ACE established that LORF and IPEP fragments are natural inhibitors for invertebrate ACE. Fifty-four percent of insect ACE activity is inhibited with 50 micro m IPEP and 35% inhibition with LORF (25 mm). Extending the peptide at both N- and C-terminus (GWEIPEPYVWDES) and the cleavage of IPEP in IP abolished the inhibitory activity of both peptides. Immunocytochemical data obtained with antisera raised against LORF and leech ACE showed a colocalization between the enzyme and its inhibitor in the same neurons. These results showed that capillary zonal electrophoresis is a useful technique for following enzymatic processes with small amounts of products and constitutes the first evidence of a natural ACE inhibitor in invertebrates.

  7. A Novel Method for Spectral Similarity Measure by Fusing Shape and Amplitude Features

    Directory of Open Access Journals (Sweden)

    J. G. Ding

    2015-12-01

    Full Text Available Spectral similarity measure is the basis of spectral information extraction. The description of spectral features is the key to spectral similarity measure. To express the spectral shape and amplitude features reasonably, this paper presents the definition of shape and amplitude feature vector, constructs the shape feature distance vector and amplitude feature distance vector, proposes the spectral similarity measure by fusing shape and amplitude features (SAF, and discloses the relationship of fusing SAF with Euclidean distance and spectral information divergence. Different measures were tested on the basis of United States Geological Survey (USGS mineral_beckman_430. Generally, measures by integrating SAF achieve the highest accuracy, followed by measures based on shape features and measures based on amplitude features. In measures by integrating SAF, fusing SAF shows the highest accuracy. Fusing SAF expresses the measured results with the inner product of shape and amplitude feature distance vectors, which integrate spectral shape and amplitude features well. Fusing SAF is superior to other similarity measures that integrate SAF, such as spectral similarity scale, spectral pan-similarity measure, and normalized spectral similarity score(NS3 .

  8. Goeckerman's therapy for psoriasis with special reference to serum pentraxin 3 level

    Energy Technology Data Exchange (ETDEWEB)

    Ctirad, A.; Lenka, B.; David, P.; Zdenek, F.; Kveta, H.; Karel, E.; Jan, K. [Charles University Prague, Hradec Kralove (Czech Republic). University Hospital

    2008-10-15

    Goeckerman's therapy (GT) of psoriasis is based on daily application of pharmacy grade coal tar on affected skin with subsequent exposure to UV light. Pentraxin 3 (PTX3) is a newly identified acute phase reactant with non redundant functions in innate immunity. PTX3 has been shown to be a reliable prognostic marker in patients with various inflammatory disorders including rheumatoid arthritis, vasculitis, and psoriasis. The aim of this study was to evaluate the influence of Goeckerman's therapy of psoriasis on levels of two pentraxins: long pentraxin PTX3 and C reactive protein in 49 patients with chronic plaque psoriasis. CRP was assessed by immunonephelometry on IMMAGE 800 (Beckman, USA). PTX3 was detected using sandwich ELISA detection set (Alexis Biochemicals, Switzerland). The serum levels of both parameters (expressed as average {+-} 1 SD) were significantly diminished after GT. The level of PTX3 dropped from 1.92 {+-} 0.72 ng/ml before GT to 1.66 {+-} 0.58 ng/ml after GT (P = 0.0396) and the level of CRP fell from 4.64 {+-} 3.93 mg/l to 1.66 {+-} 0.58 mg/l (P {lt} 0.0001). Comparing to healthy controls, the serum levels of both parameters before GT were significantly higher than those found in healthy blood donors and remained significantly increased after GT. Increased serum concentrations of pentraxin 3 and CRP are alleviated by GT in patients with psoriasis.

  9. An application of the theory of planned behavior to examine the impact of classroom inclusion on elementary school students.

    Science.gov (United States)

    Campbell, Michael

    2010-05-01

    Classroom inclusion serves as the most discussed service delivery model in the debate over the most appropriate way to provide education for students with disabilities. Integrating students with disabilities with nondisabled peers may increase attitudes of acceptance, but the literature also indicates that placement alone does not yield an increase in interaction between these two groups of peers (Brinker & Thorpe, 1986; Fryexe & Kennedy, 1995; Kennedy, Shulka, & Fryxell, 1997). This study investigated the impact of classroom inclusion on nondisabled students. Using survey research methods and guided by the Theory of Planned Behavior (Aizen, I. [1985]. From intentions to actions: A theory of planned behavior. In J. Kuhl & J. Beckman [Eds.], Action-control: From cognition to behavior [pp. 11-39]. Heidelberg: Springer), 593 responses were obtained from a convenience sample of 936 third, fourth, and fifth grade students and their parents from 52 classrooms spread across six different schools. Survey results were also collected from these students' parents and their teachers and used to add a richer depth to the data analysis. Implications for policy and practice are drawn. Limitations and recommendations for future research are also indicated. PMID:20526942

  10. Cytotoxic effect of Erythroxylum suberosum combined with radiotherapy in head and neck cancer cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Macedo, Taysa B.C.; Torres, Hianne M.; Yamamoto-Silva, Fernanda Paula; Silva, Maria Alves G. [Universidade Federal de Goias (UFG), Goiania, GO (Brazil). Escola de Odontologia; Elias, Silvia T.; Silveira, Damaris; Magalhaes, Perola O.; Lofrano-Porto, Adriana; Guerra, Eliete N.S., E-mail: elieteneves@unb.br [Universidade de Brasilia (UnB), Brasilia, DF (Brazil). Faculdade de Ciencias da Saude

    2016-01-15

    The mouth and oropharynx cancer is the 6{sup th} most common type of cancer in the world. The treatment may involve surgery, chemotherapy and radiotherapy. More than 50% of drugs against cancer were isolated from natural sources, such as Catharanthus roseus and epipodophyllotoxin, isolated from Podophyllum. The biggest challenge is to maximize the control of the disease, while minimizing morbidity and toxicity to the surrounding normal tissues. The Erythroxylum suberosum is a common plant in the Brazilian Cerrado biome and is popularly known as 'cabelo-de-negro'. The objective of this study was to evaluate the cytotoxic activity of Erythroxylum suberosum plant extracts of the Brazilian Cerrado biome associated with radiotherapy in human cell lines of oral and hypopharynx carcinomas. Cells were treated with aqueous, ethanolic and hexanic extracts of Erythroxylum suberosum and irradiated at 4 Gy, 6 Gy and 8 Gy. Cytotoxicity was evaluated by MTT assay and the absorbance was measured at 570 nm in a Beckman Counter reader. Cisplatin, standard chemotherapy, was used as positive control. The use of Erythroxylum suberosum extracts showed a possible radiosensitizing effect in vitro for head and neck cancer. The cytotoxicity effect in the cell lines was not selective and it is very similar to the effect of standard chemotherapy. The aqueous extract of Erythroxylum suberosum, combined with radiotherapy was the most cytotoxic extract to oral and hypopharynx carcinomas. (author)

  11. Blood flow dynamics after laser therapy of port wine stain birthmarks

    Science.gov (United States)

    Huang, Yu-Chih; Tran, Nadia; Ross, E. Victor; Shumaker, Peter R.; Nelson, J. Stuart; Kelly, Kristen; Choi, Bernard

    2009-02-01

    During laser therapy of port wine stain (PWS) birthmarks, regions of persistent perfusion may exist. We hypothesize that such regions, which are not readily visible, exist even during laser surgery performed by highly experienced clinicians. The objective of this study was to use objective feedback to assess the acute vascular response to laser therapy. We have developed a clinic-friendly laser speckle imaging (LSI) instrument to provide the clinician with real-time images of blood flow during laser therapy. We acquired images from patients undergoing laser therapy of PWS birthmarks at Scripps Clinic and Beckman Laser Institute and Medical Clinic. We extracted blood flow maps from the acquired imaging data. Collectively, we have observed two regimes of patient response to therapy: 1) an immediate increase in perfusion within minutes after laser therapy; and 2) an overall decrease in blood perfusion approximately one hour after laser therapy, with distinct regions of persistent perfusion apparent in the majority of post-treatment blood-flow images. A comparison of blood flow in PWS and adjacent normal skin demonstrated that PWS blood flow can be greater than or sometimes equivalent to that of normal skin. Regions of persistent perfusion frequently exist immediately after laser therapy of PWS birthmarks. Existence of these regions may be correlated to the need for multiple treatment sessions to improve substantially PWS skin appearance. With the use of intraoperative LSI, immediate retreatment of these regions may improve the outcome of each session.

  12. Color Parameters of the Chromascop Shade Guide

    Directory of Open Access Journals (Sweden)

    William J. O'Brien

    2012-12-01

    Full Text Available The purposes of this study are: (1 determine the color of the twenty shades in the Ivoclar’s Chromascop shade guide, (2 determine the color representation of the shade guide described as coverage error (CE, and (3 compare this shade guide with the Vita Classical and Bioform shade guides. The spectral data was collected using Beckman model DU reflectance spectrophotometer equipped with an integrating sphere. Commission International de l’Eclairage (CIE chromaticity coordinates were calculated using CIE illuminant C and 1931 observer data, then converted to CIE L*a*b* and Munsell notation. Each shade was spectrophotometrically compared to the published colors of 335 human teeth. The minimum CIE L*a*b* color difference was calculated for each tooth and the average of these color differences was defined as the CE. The measured colors of the Chromascop guide had a CIE L* range of 79.67 to 65.61, an a* range of -0.71 to 3.85, and a b* range of 14.58 to 27.69. The average CE of the Chromascop shade guide was 3.38. The Chromascop shade guide has similar colors and a CE compared with the Bioform and Vita Classical shade guides, but with some shades of higher red and yellow components.

  13. Graphene Quantum Point Contact Transistor for DNA Sensing

    Science.gov (United States)

    Girdhar, Anuj

    2014-03-01

    Over the past few years the need has grown for low-cost, high-speed, and accurate biomolecule sensing technology. Graphene is a promising choice for use in such sensing applications, as its single-atom thickness and unique electronic structure is suitable for probing biomolecules like DNA at a very high resolution. We propose the design of a transistor containing a graphene nanoribbon sensing layer with a nanopore for the simultaneous detection and control of a translocating DNA molecule. Through the combination of molecular dynamics simulations, a self-consistent Poisson equation solver, and electronic transport theory, we show that the motion of a DNA molecule through a nanopore can be observed by measuring conductance modulations in the graphene nanoribbon. We also demonstrate that the sensitivity of the graphene sheet conductance to external charges can be enhanced by modulating its carrier concentration as well as by choosing a quantum point contact geometry for the graphene nanoribbon. In addition, we propose the use of extra gates to control both the lateral and translocating motion of a DNA molecule inside the nanopore. I would like to acknowledge Oxford Nanopore Technology as well as the Beckman Institute for Advanced Science for their support.

  14. Synthesis of immunomagnetic nanoparticles and their application in the separation and purification of CD34 + hematopoietic stem cells

    Science.gov (United States)

    Chen, Wei; Shen, Hebai; Li, Xingyu; Jia, Nengqin; Xu, Jianming

    2006-12-01

    The silica-coated superparamagnetic nanoparticles with the uniform diameter of about 60 nm were synthesized by reverse microemulsions method. And the magnetic nanoparticles were modified with N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (AEAPS). The immunomagnetic nanoparticles were then successfully prepared by covalently immobilizing anti-CD34 + monoclonal antibodies to the surface of amino silane modified magnetic particles. The cell separation results showed that the synthesized immunomagnetic nanoparticles could rapidly and conveniently separate the CD34 + cells with high efficiency and specificity than normal ones. The surface morphology of separated target cells was examined by scanning electron microscope (SEM). Atomic force microscope (AFM) also characterized the magnetic materials on the surface of the separated target cells for the first time, which further confirmed that the target cells were separated by the immunomagnetic nanoparticles. The viability of the separated cells was studied by culturing method and Beckman Vi-cell viability analyst. Therefore, our experiments provided a new, direct, rapid mode to separate target cells.

  15. Encapsulation of Platelet in Kefiran Polymer and Detection of Bioavailability of Immobilized Platelet in Probiotic Kefiran as A New Drug for Surface Bleeding

    Directory of Open Access Journals (Sweden)

    Anahita Jenab

    2015-11-01

    Full Text Available Background : Kefir contains lactic acid bacteria (Lactobacillus, Lactococcus, Leuconostoc, Acetobacter and Streptococcus and yeasts (Kluyveromyces, Torula, Candida, Saccharomyces .Kefiran is the polysaccharide produced by lactic acid bacteria in kefir.Methods : Kefiran was prepared from milk containing 0.5% fat and 10 grams kefir grains and was separated from kefir by ethanol (0.02 gram following entrapping the platelets to this polymer. Ligand of the platelet-polysaccharide was studied by FTIR.Results : FTIR results showed that the bands of C-O and C-O-C connections were formed and the polysaccharides had been attached to the receptors of the platelet glycoproteins (GP Ib,GPIIb / IIIa. Stability and encapsulation of the platelet and kefiran were assessed by Coulter Counter. Encapsulation of the platelets by polysaccharide at the beginning caused to reduce the number of platelets following by releasing of 50% of the platelets after 3 hours.Conclusion : The platelets were encapsulated in kefiran polymer and detected for bioavailability as new drug for surface bleeding. Also, kefiran has antimicrobial and antifungal properties. On the other hand, the existence of nisin in kefiran could be useful as an antibacterial lantibiotic. 

  16. Transcellular ion flow in Escherichia coli B and electrical sizing of bacterias.

    Science.gov (United States)

    Zimmermann, U; Schulz, J; Pilwat, G

    1973-10-01

    Dielectric breakdown of cell membranes and, in response, transcellular ion flows were measured in Escherichia coli B 163 and B 525 using a Coulter counter as the detector with a hydrodynamic jet focusing close to the orifice of the counter. Plotting the relative pulse height for compensated amplification of a certain size of the cells against increasing detector current, a rather sharp bend within the linear function was found, which did not occur when measuring fixed cells or polystyrene latex. The start current for transcellular ion flow causing the change of the slope is different for the potassium-deficient mutant B 525 in comparison with the wild-type B 163, indicating a change in the membrane structure of B 525 by mutation and demonstrating the sensitivity of the method for studying slight changes in membrane structure in general. The theoretical size distributions for two current values in the range of transcellular ion flow were constructed from the true size distribution at low detector currents, assuming an idealized sharp changeover of the bacterial conductivity from zero to one-third of the electrolyte conductivity.

  17. Standard methods for chemical, mass spectrometric, and spectrochemical analysis of, and physical tests on, beryllium oxide powder

    International Nuclear Information System (INIS)

    Methods are described for the determination of C, Fe, N, Cl-, Li, S, thirty trace elements, and the amount of BeO in BeO powder. Methods are included for certain physical properties of the oxide powders. C is determined by the combustion-thermal conductivity method. A colorimetric method using orthophenanthroline is used for the determination of Fe, and N is determined by a micro Kjeldahl method. Chlorine is determined nephlometrically using AgNO3, and Li is determined by atomic absorption spectrophotometry. A complete burning spectrochemical method and a spark-source mass spectrographic method were used to analyze for the thirty trace elements. The density of the BeO powders was measured by a toluene displacement method, and a tap-pak volumetric method was used for the determination of the pour and tap densities. Two different methods described for the particle-size composition determination are Coulter Counter method and sieve analysis method. Methods for determination of bulk density, real density, porosity, and pore size of the solid oxides are described. A nitrogen absorption method is described for the determination of surface area

  18. Automated platelet counters: a comparative evaluation of latest instrumentation.

    Science.gov (United States)

    Mayer, K; Chin, B; Magnes, J; Thaler, H T; Lotspeich, C; Baisley, A

    1980-08-01

    An extensive evaluation of performance characteristics and accuracy of clinical results for two automated multiparameter whole-blood cell counters (the Coulter Counter Model S-Plus and the Ortho ELT-8) and two single-parameter semiautomated platelet counters (the J. T. Baker MK-4/HC and the Clay-Adams Ultra-Flo 100) is described. Results of comparative assays performed on more than 1,200 clinical specimens are analyzed. These results are compared with manual determinations where appropriate. Particular attention is accorded to the accuracy of platelet counts, especially at abnormal levels below 70 X 10(3)/cu mm, where falsely elevated platelet counts may lead to serious clinical consequences. Both multiparameter instruments yielded accurate results, with the exception of low values reported by the ELT-8 for mean corpuscular volumes above 100 cu micrometer. Results for platelet counts were accurate for most specimens on all four instruments; the ELT-8 was the most reliable (P < 0.01), especially for the critically low counts. Although no instrument is infallible in determining platelet counts at all levels, the authors conclude that addition of platelet-counting capability represents a significant advancement over existing instrumentation. PMID:7405891

  19. A side-by-side evaluation of four platelet-counting instruments.

    Science.gov (United States)

    Dalton, W T; Bollinger, P; Drewinko, B

    1980-08-01

    The performances of four instruments for counting platelets were evaluated in a side-by-side study: the Haema-Count MK-4/HC, an electronic impedance instrument that counts platelets in platelet-rich plasma; the Ultra-Flo 100, and the Coulter Counter Model S-Plus, electronic impedance instruments that count platelets in the presence of intact erythrocytes; and the AutoCounter, an optical instrument that counts platelets in the presence of lysed erythrocytes. The Ultra-Flo 100 and the S-Plus showed the best within-run precision, and all four instruments were considerably more precise than manual platelet counting, especially at low levels of platelet count. The four instruments were all linear in the ranges tested (5 to 650 x 10(9)/or greater), and sample carry-over was less than 0.7% for each. A noteworthy finding was that the erythrocyte concentration of the blood samples affected the displayed platelet count of the S-Plus and, to a lesser extent, that of the AutoCounter, in a predictable way, whereas it did not greatly affect the displayed count of the Ultra-Flo 100. In addition to differences in quality of performances, the four instruments differed considerably in speed and ease of operation and in cost. PMID:7405890

  20. Detection of hydrogen peroxide with chemiluminescent micelles

    Directory of Open Access Journals (Sweden)

    Dongwon Lee

    2008-08-01

    Full Text Available Dongwon Lee1, Venkata R Erigala1,3, Madhuri Dasari1, Junhua Yu2, Robert M Dickson2, Niren Murthy11The Wallace H. Coulter Department of Biomedical Engineering; 2Department of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA; 3The Scripps Research Institute, La Jolla, CA, USAAbstract: The overproduction of hydrogen peroxide is implicated in the progress of numerous life-threatening diseases and there is a great need for the development of contrast agents that can detect hydrogen peroxide in vivo. In this communication, we present a new contrast agent for hydrogen peroxide, termed peroxalate micelles, which detect hydrogen peroxide through chemiluminescence, and have the physical/chemical properties needed for in vivo imaging applications. The peroxalate micelles are composed of amphiphilic peroxalate based copolymers and the fluorescent dye rubrene, they have a ‘stealth’ polyethylene glycol (PEG corona to evade macrophage phagocytosis, and a diameter of 33 nm to enhance extravasation into permeable tissues. The peroxalate micelles can detect nanomolar concentrations of hydrogen peroxide (>50 nM and thus have the sensitivity needed to detect physiological concentrations of hydrogen peroxide. We anticipate numerous applications of the peroxalate micelles for in vivo imaging of hydrogen peroxide, given their high sensitivity, small size, and biocompatible PEG corona.Keywords: hydrogen peroixde, chemiluminescence, micelles, amphiphilic copolymer

  1. Particle size distribution of cataclastic fault materials from Southern California: A 3-D study

    Science.gov (United States)

    An, Lin-Ji; Sammis, Charles G.

    1994-03-01

    The particle size distributions of fault gouge from the San Andreas, the San Gabriel, and the Lopez Canyon faults in Southern California were measured using sieving and Coulter-Counter techniques over a range of particle sizes from 2 μm to 16 mm. The distributions were found to be power law (fractal) for the smaller fragments and log-normal by mass for sizes near and above the peak size. The apparent fractal dimension D of the smaller particles in gouge samples from the San Andreas fault, the San Gabriel fault and the Lopez Canyon gouge were 2.4 3.6, 2.6 2.9 and 2.4 3.0, respectively. The average D for the Lopez Canyon gouge was 2.7±0.2, which is in agreement with earlier studies of this gouge using planar 2-D sections. The fractal dimension of the finer fragments from all three faults is observed to be correlated with the peak fragment size, with finer gouges tending to have a larger D. A computer automaton is used to show that this observation may be explained as resulting from a fragmentation process which has a “grinding limit” at which particle reduction stops.

  2. How does the greater white-toothed shrew, Crocidura russula, responds to long-term heavy metal contamination? - A case study

    Energy Technology Data Exchange (ETDEWEB)

    Marques, Carla Cristina [Centro de Biologia Ambiental, Departamento Biologia Animal, Faculdade de Ciencias, Universidade Lisboa, Campo Grande, 1749-016 Lisbon (Portugal)]. E-mail: ccmarques@fc.ul.pt; Sanchez-Chardi, Alejandro [Departament Biologia Animal (Vertebrats), Facultat de Biologia, Universitat Barcelona, Av. Diagonal 645, 08028 Barcelona (Spain); Gabriel, Sofia Isabel [Centro de Biologia Ambiental, Departamento Biologia Animal, Faculdade de Ciencias, Universidade Lisboa, Campo Grande, 1749-016 Lisbon (Portugal); Nadal, Jacint [Departament Biologia Animal (Vertebrats), Facultat de Biologia, Universitat Barcelona, Av. Diagonal 645, 08028 Barcelona (Spain); Viegas-Crespo, Ana Maria [Centro de Biologia Ambiental, Departamento Biologia Animal, Faculdade de Ciencias, Universidade Lisboa, Campo Grande, 1749-016 Lisbon (Portugal); Luz Mathias, Maria da [Centro de Biologia Ambiental, Departamento Biologia Animal, Faculdade de Ciencias, Universidade Lisboa, Campo Grande, 1749-016 Lisbon (Portugal)

    2007-04-15

    Heavy metals accumulation in parallel with the evaluation of physiological and biochemical effects resulting from continued metal exposure were considered here using for the first time the great white-toothed shrew Crocidura russula as an in vivo model. Shrews were originated from an abandoned lead/zinc mining area and from a reference area, both in Alentejo, southern Portugal. Hepatic contents of nickel, copper, zinc, cadmium, mercury and lead were quantified by Inductively Coupled Plasma Mass Spectrometer (ICP-MS). Haematological parameters (white blood cells, red blood cells, haemoglobin and haematocrit) were obtained in a Coulter Counter Analyser and biochemical markers of the redox balance (glutathione S-transferase, glutathione peroxidase, and glutathione reductase) activities were measured spectrophotometrically using a Duo-50 spectrophotometer. Compared with control animals, significantly higher concentration of hepatic cadmium (9.29 vs. 1.18 {mu}g/g dry weight) and nickel (1.56 vs. 0.343 {mu}g/g dry weight) were detected in the shrews collected in the mining area. However, no significant changes were observed on haematological or enzymatic parameters in animals exposed to metal pollution. The obtained results show that shrews are good bioaccumulators of toxic heavy metals, but very tolerant to their effects, revealing an interesting long-term adaptation to polluted environments. In addition, this study provides reference values for haematological parameters and antioxidant enzymes levels in C. russula, which may be relevant for comparative purposes in further studies.

  3. Characterization of winemaking yeast by cell number-size distribution analysis through flow field-flow fractionation with multi-wavelength turbidimetric detection.

    Science.gov (United States)

    Zattoni, Andrea; Melucci, Dora; Reschiglian, Pierluigi; Sanz, Ramsés; Puignou, Lluís; Galceran, Maria Teresa

    2004-10-29

    Yeasts are widely used in several areas of food industry, e.g. baking, beer brewing, and wine production. Interest in new analytical methods for quality control and characterization of yeast cells is thus increasing. The biophysical properties of yeast cells, among which cell size, are related to yeast cell capabilities to produce primary and secondary metabolites during the fermentation process. Biophysical properties of winemaking yeast strains can be screened by field-flow fractionation (FFF). In this work we present the use of flow FFF (FlFFF) with turbidimetric multi-wavelength detection for the number-size distribution analysis of different commercial winemaking yeast varieties. The use of a diode-array detector allows to apply to dispersed samples like yeast cells the recently developed method for number-size (or mass-size) analysis in flow-assisted separation techniques. Results for six commercial winemaking yeast strains are compared with data obtained by a standard method for cell sizing (Coulter counter). The method here proposed gives, at short analysis time, accurate information on the number of cells of a given size, and information on the total number of cells.

  4. Struvite crystalluria and urolithiasis in cross Labradors.

    Science.gov (United States)

    Rodgers, A L; Irving, R A; Mezzabotta, M; Benloulou, P

    1986-01-01

    Recurrent struvite crystalluria and urolithiasis in a cross-Labrador bitch was studied using a combined Coulter-Counter and scanning electron microscope (SEM) approach. Staphylococcus bacteria were cultured from the patient's urine as well as from the calculi themselves. Urine samples were subjected to particle counting and sizing during active and non-active periods of stone formation. Size distribution curves so obtained were identical as were those derived from sterile and non-sterile specimens. These showed a peak incidence at a diameter of 5 microns. Particle sizes for 6 controls were also determined and showed an even distribution over a much wider range with small peaks occurring at 3, 10, and 20 microns diameters. SEM studies of urine sediments revealed the presence of struvite crystals in all the controls as well as in the stone-former. These occurred in a variety of shapes and sizes but were generally larger in the controls. SEM also revealed intimate admixtures of struvite and apatite in calculi surgically removed from the patient. The results of this study indicate that crystal numbers are of greater significance than crystal size. It is also suggested that Cross-Labradors may be unusually predisposed to struvite crystalluria. The repeated recurrence of struvite urolithiasis in the subject indicates a possible inherent physiological malfunction in the animal's ability to cope with this crystalluria. The absence of a nucleation inhibitor in the stone-former's urine is also postulated.

  5. Prehistoric peyote use: alkaloid analysis and radiocarbon dating of archaeological specimens of Lophophora from Texas.

    Science.gov (United States)

    El-Seedi, Hesham R; De Smet, Peter A G M; Beck, Olof; Possnert, Göran; Bruhn, Jan G

    2005-10-01

    Two archaeological specimens of peyote buttons, i.e. dried tops of the cactus Lophophora williamsii (Lem.) Coulter, from the collection of the Witte Museum in San Antonio, was subjected to radiocarbon dating and alkaloid analysis. The samples were presumably found in Shumla Cave No. 5 on the Rio Grande, Texas. Radiocarbon dating shows that the calibrated 14C age of the weighted mean of the two individual dated samples corresponds to the calendric time interval 3780-3660 BC (one sigma significance). Alkaloid extraction yielded approximately 2% of alkaloids. Analysis with thin-layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS) led to the identification of mescaline in both samples. No other peyote alkaloids could be identified. The two peyote samples appear to be the oldest plant drug ever to yield a major bioactive compound upon chemical analysis. The identification of mescaline strengthens the evidence that native North Americans recognized the psychotropic properties of peyote as long as 5700 years ago.

  6. Peyote identification on the basis of differences in morphology, mescaline content, and trnL/trnF sequence between Lophophora williamsii and L. diffusa.

    Science.gov (United States)

    Aragane, Masako; Sasaki, Yohei; Nakajima, Jun'ichi; Fukumori, Nobutaka; Yoshizawa, Masao; Suzuki, Yukiko; Kitagawa, Shigemi; Mori, Ken'ichiro; Ogino, Shuzo; Yasuda, Ichiro; Nagumo, Seiji

    2011-01-01

    Genus Lophophora (Cactaceae) has two species: Lophophora williamsii Coulter, which is called peyote, and L. diffusa Bravo. Although it was reported that L. williamsii contained mescaline and L. diffusa did not, we found L. williamsii specimens that did not contain mescaline. This finding indicated that the two species could not be differentiated in terms of mescaline content. Moreover, the relationship between mescaline content and morphology of the two species is also unknown. In this study, we attempted to clarify the difference in morphology, mescaline content, and DNA alignment of the chloroplast trnL/trnF region between L. williamsii and L. diffusa. As a result, L. williamsii specimens were classified into two groups. Group 1 had small protuberances on the epidermis, contained mescaline, and the analyzed region on the trnL/trnF sequence was 881 base pairs (bp) long in all except one (877 bp). Group 2 had large protuberances on the epidermis, did not contain mescaline, and the analyzed region was 893 bp long. On the other hand, L. diffusa had medium-sized protuberances on the epidermis, did not contain mescaline, and the analyzed region was 903 bp long. Also investigated was the potential application of the PCR-restriction fragment length polymorphism (RFLP) method as a means of identification based on the trnL/trnF sequence. By applying the PCR-RFLP method, the two species could be distinguished and L. williamsii specimens could be differentiated into group 1 and group 2.

  7. Bromelain proteases reduce human platelet aggregation in vitro, adhesion to bovine endothelial cells and thrombus formation in rat vessels in vivo.

    Science.gov (United States)

    Metzig, C; Grabowska, E; Eckert, K; Rehse, K; Maurer, H R

    1999-01-01

    The thiol protease, bromelain, an extract from pineapple stem, was suggested to have antithrombotic and anticoagulant activities in vivo. We studied the effects of bromelain on cell size distribution of isolated human platelets in vitro by Coulter Counter measurements. Preincubation of platelets with bromelain (10 micrograms/mL) completely prevented the thrombin (0.2 U/mL) induced platelet aggregation. Papain was less active in preventing platelet aggregation. In vitro, bromelain (0.1 microgram/mL) reduced the adhesion of bound, thrombin stimulated, fluorescent labeled platelets to bovine aorta endothelial cells. In addition, preincubation of platelets with bromelain, prior to thrombin, activation, reduced the platelet adhesion to the endothelial cells to the low binding value of unstimulated platelets. On the basis of mass concentrations, the proteases papain and trypsin were as effective as bromelain. Using a laser thrombosis model, the in vivo effects of orally and intraveneously applied bromelain on thrombus formation in rat mesenteric vessels were studied. Bromelain, orally applied at 60 mg/kg body weight, inhibited the thrombus formation in a time dependent manner, the maximum being after 2 hours in 11% of arterioles and 6% of venoles. Intravenous application at 30 mg/kg was slightly more active in reducing thrombus formation in arterioles (13%) and venoles (5%), suggesting that orally applied bromelain is biologically active. These results may help to explain some of the clinical effects observed after bromelain treatment in patients with thrombosis and related diseases.

  8. Cytotoxic effect of the pentacyclic oxindole alkaloid mitraphylline isolated from Uncaria tomentosa bark on human Ewing's sarcoma and breast cancer cell lines.

    Science.gov (United States)

    García Giménez, Dolores; García Prado, Elena; Sáenz Rodríguez, Teresa; Fernández Arche, Angeles; De la Puerta, Rocío

    2010-02-01

    Preparations from Uncaria tomentosa, a South American Rubiaceae, have been used in the Peruvian traditional medicine for the treatment of infective, inflammatory and tumoral processes. In this study, the pentacyclic oxindole alkaloid mitraphylline was isolated from the dried inner bark of this plant species, and its structure elucidated by analysis of NMR spectroscopic data. Mitraphylline was differentially identified from its stereoisomeric pair isomitraphylline by (15)N-NMR. Its antiproliferative and cytotoxic effects have been tested on human Ewing's sarcoma MHH-ES-1 and breast cancer MT-3 cell lines, using cyclophosphamide and vincristine as reference controls. A Coulter counter was used to determine viable cell numbers, followed by the application of the tetrazolium compound MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy phenyl)-2-(4-sulfophenyl)-2H-tetrazolium] an inner salt. A colorimetric method was employed to evaluate cell viability in this cytotoxic assay. Micromolar concentrations of mitraphylline (5 microM to 40 microM) inhibited the growth of both cell lines in a dose-dependent manner. The IC (50) +/- SE values were 17.15 +/- 0.82 microM for MHH-ES-1 and 11.80 +/- 1.03 microM for MT-3 for 30 hours, smaller than those obtained for the reference compounds. This action suggests that the pentacyclic oxindole alkaloid mitraphylline might be a new promising agent in the treatment of both human sarcoma and breast cancer. PMID:19724995

  9. Diffusion controlled deposition of particulate matter from flowing slurries

    International Nuclear Information System (INIS)

    Investigations have been carried out to determine the rate of deposition of micron sized magnetite particles from flowing slurry onto the inner walls of simulated heat exchanger tubes. Slurry was circulated in a closed-loop facility with deposition measurements being made in an aluminium tube of 19mm bore. Runs were conducted under isothermal conditions at temperatures between 293K and 350K over a Reynolds Number range 9,600 to 147,000. Slurry concentration was maintained in the range 200 to 600 mg/kg. The average magnetite particle size as measured on a Coulter Counter was approximately 1.5 μm. Deposit growth on the test section was measured by means of a low energy X-ray machine. The results showed high initial deposition rates accompanied by steadily increasing removal rates producing asymptotic curves. Analysis indicates that deposition is governed by the forced convective diffusion of particles to the tube wall. Correlation of deposition rates is possible using standard mass transfer equations. Deposits were friable and porous with void fractions ≅70%

  10. Evaluation of the accuracy of the diff3/50 with a cell by cell comparison.

    Science.gov (United States)

    Cembrowski, G S; Wilson, M K; Adelmeyer, S G; Chang, C K

    1983-09-01

    The traditional approach to the evaluation of accuracy of discrete differential counters uses a graphic comparison of automated and manual counts. This type of comparison introduces so much sampling variation that significant differences often are obscured. To exclude this sampling variability, one of three technologists, a reference technologist and the Coulter diff3/50, each performed a differential count on exactly the same 100 cells of 47 normal and 56 abnormal blood smears. Linear regression analysis demonstrated excellent agreement between the technologists for all cell types with the exception of band neutrophils. Comparison of the instrument results with the technologists' results showed very good agreement for segmented neutrophils, lymphocytes, monocytes, and eosinophils. The identification of band neutrophils, basophils, immature granulocytes and atypical lymphocytes requires improvement. The overall performance is acceptable, with only 11 leukocytes, on the average normal smear, misclassified out of 100 normal leukocytes (four bands called segs, four segs called bands, and three non-neutrophils incorrectly classified). We recommend similar evaluations for all discrete differential counters.

  11. Comparison of three different methods of total carbon dioxide measurement.

    Science.gov (United States)

    Kilborn, Susan H.; Bonnett, Brenda N.; Pook, Harold A.

    1995-01-01

    The objective of this study was to compare total carbon dioxide (TCO(2)) levels measured by three different methods. Two hundred jugular venous blood samples from dogs admitted to the Veterinary Teaching Hospital, Ontario Veterinary College with various clinical disorders were analyzed by the Radiometer blood gas analyzer (BGA) and the Coulter DACOS analyzer. In 70 of these samples, TCO(2) was also measured by the Kodak Ektachem DTE chemistry analyzer. Comparison of the agreement between methods revealed intraclass correlation coefficients (ICC) of 0.63, 0.79, and 0.82 for the DACOS-Ektachem, DACOS-BGA and Ektachem-BGA comparisons, respectively. Evaluation of the effect of storage time on TCO(2) content of stored serum samples measured on the DACOS analyzer revealed a decrease of almost 2 mmol/L and a decrease in the intraclass correlation coefficient values between the DACOS analyzer and other methods after 7 hours storage time. The results of this observational study revealed lower agreement between the three methods in this study than previously reported between other methods of TCO(2) measurement. Possible reasons for the lower than expected agreement in this study included changes in DACOS values because of storage and differences in methodologies between the methods.

  12. Microfluidic CODES: a scalable multiplexed electronic sensor for orthogonal detection of particles in microfluidic channels.

    Science.gov (United States)

    Liu, Ruxiu; Wang, Ningquan; Kamili, Farhan; Sarioglu, A Fatih

    2016-04-21

    Numerous biophysical and biochemical assays rely on spatial manipulation of particles/cells as they are processed on lab-on-a-chip devices. Analysis of spatially distributed particles on these devices typically requires microscopy negating the cost and size advantages of microfluidic assays. In this paper, we introduce a scalable electronic sensor technology, called microfluidic CODES, that utilizes resistive pulse sensing to orthogonally detect particles in multiple microfluidic channels from a single electrical output. Combining the techniques from telecommunications and microfluidics, we route three coplanar electrodes on a glass substrate to create multiple Coulter counters producing distinct orthogonal digital codes when they detect particles. We specifically design a digital code set using the mathematical principles of Code Division Multiple Access (CDMA) telecommunication networks and can decode signals from different microfluidic channels with >90% accuracy through computation even if these signals overlap. As a proof of principle, we use this technology to detect human ovarian cancer cells in four different microfluidic channels fabricated using soft lithography. Microfluidic CODES offers a simple, all-electronic interface that is well suited to create integrated, low-cost lab-on-a-chip devices for cell- or particle-based assays in resource-limited settings.

  13. Cytotoxic effect of the pentacyclic oxindole alkaloid mitraphylline isolated from Uncaria tomentosa bark on human Ewing's sarcoma and breast cancer cell lines.

    Science.gov (United States)

    García Giménez, Dolores; García Prado, Elena; Sáenz Rodríguez, Teresa; Fernández Arche, Angeles; De la Puerta, Rocío

    2010-02-01

    Preparations from Uncaria tomentosa, a South American Rubiaceae, have been used in the Peruvian traditional medicine for the treatment of infective, inflammatory and tumoral processes. In this study, the pentacyclic oxindole alkaloid mitraphylline was isolated from the dried inner bark of this plant species, and its structure elucidated by analysis of NMR spectroscopic data. Mitraphylline was differentially identified from its stereoisomeric pair isomitraphylline by (15)N-NMR. Its antiproliferative and cytotoxic effects have been tested on human Ewing's sarcoma MHH-ES-1 and breast cancer MT-3 cell lines, using cyclophosphamide and vincristine as reference controls. A Coulter counter was used to determine viable cell numbers, followed by the application of the tetrazolium compound MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy phenyl)-2-(4-sulfophenyl)-2H-tetrazolium] an inner salt. A colorimetric method was employed to evaluate cell viability in this cytotoxic assay. Micromolar concentrations of mitraphylline (5 microM to 40 microM) inhibited the growth of both cell lines in a dose-dependent manner. The IC (50) +/- SE values were 17.15 +/- 0.82 microM for MHH-ES-1 and 11.80 +/- 1.03 microM for MT-3 for 30 hours, smaller than those obtained for the reference compounds. This action suggests that the pentacyclic oxindole alkaloid mitraphylline might be a new promising agent in the treatment of both human sarcoma and breast cancer.

  14. Soil structure and greenhouse gas production differences between row and interrow positions under no-tillage

    Directory of Open Access Journals (Sweden)

    Alvaro Pires da Silva

    2014-04-01

    Full Text Available No-tillage in Brazil is an efficient agricultural system that improves crop productivity whilst controlling erosion caused to the soil by degradation. However, there is some concern regarding soil compaction. Our objective was to determine whether the function of soil structure in sustaining crop growth was dependent on row and interrow positions in long-term no-tillage. We took soil samples from a field in a commercial farm under long-term no-tillage since 1979 on a clayey Oxisol in Southern Brazil. We assessed soil physical quality using the revised Peerlkamp technique and measured bulk density, air-filled porosity and air permeability of intact soil cores. Samples were incubated to assess in vitro N2O and CO2 production. The soil physical and structural properties showed consistent differences between interrow and row positions, where the properties measured were more favorable. The revised Peerlkamp technique proved as efficient as quantitative parameters in discriminating treatment differences. Overall, soil physical conditions in the interrow were less favourable than in the row. Pore continuity did not vary as regards position. This may explain why row position did not influence in vitro N2O and CO2 production. Soil physical quality under no-tillage system is enhanced, at least in the short term, by superficial disturbances in the row as a result of the action of the coulters of the no-tillage seeder.

  15. Single Nanoparticle Translocation Through Chemically Modified Solid Nanopore.

    Science.gov (United States)

    Tan, Shengwei; Wang, Lei; Liu, Hang; Wu, Hongwen; Liu, Quanjun

    2016-12-01

    The nanopore sensor as a high-throughput and low-cost technology can detect single nanoparticle in solution. In the present study, the silicon nitride nanopores were fabricated by focused Ga ion beam (FIB), and the surface was functionalized with 3-aminopropyltriethoxysilane to change its surface charge density. The positively charged nanopore surface attracted negatively charged nanoparticles when they were in the vicinity of the nanopore. And, nanoparticle translocation speed was slowed down to obtain a clear and deterministic signal. Compared with previous studied small nanoparticles, the electrophoretic translocation of negatively charged polystyrene (PS) nanoparticles (diameter ~100 nm) was investigated in solution using the Coulter counter principle in which the time-dependent nanopore current was recorded as the nanoparticles were driven across the nanopore. A linear dependence was found between current drop and biased voltage. An exponentially decaying function (t d   ~ e (-v/v0) ) was found between the duration time and biased voltage. The interaction between the amine-functionalized nanopore wall and PS microspheres was discussed while translating PS microspheres. We explored also translocations of PS microspheres through amine-functionalized solid-state nanopores by varying the solution pH (5.4, 7.0, and 10.0) with 0.02 M potassium chloride (KCl). Surface functionalization showed to provide a useful step to fine-tune the surface property, which can selectively transport molecules or particles. This approach is likely to be applied to gene sequencing. PMID:26831688

  16. Mineral Analysis of Pine Nuts (Pinus spp. Grown in New Zealand

    Directory of Open Access Journals (Sweden)

    Leo P. Vanhanen

    2013-04-01

    Full Text Available Mineral analysis of seven Pinus species grown in different regions of New Zealand; Armand pine (Pinus armandii Franch, Swiss stone pine (Pinus cembra L., Mexican pinyon (Pinus cembroides Zucc. var. bicolor Little, Coulter pine (Pinus coulteri D. Don, Johann’s pine (Pinus johannis M.F. Robert, Italian stone pine (Pinus pinea L. and Torrey pine (Pinus torreyana Parry ex Carrière, was carried out using an inductively coupled plasma optical emission spectrophotometer (ICP-OES analysis. Fourteen different minerals (Al, B, Ca, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, S and Zn were identified in all seven varieties, except that no Al or Na was found in Pinus coulteri D. Don. New Zealand grown pine nuts are a good source of Cu, Mg, Mn, P and Zn, meeting or exceeding the recommended RDI for these minerals (based on an intake of 50 g nuts/day while they supplied between 39%–89% of the New Zealand RDI for Fe. Compared to other commonly eaten tree-nuts New Zealand grown pine nuts are an excellent source of essential minerals.

  17. Identification and evaluation of biological control potential of B-401 endophytic bacteria in grasses on alpine grasslands%高寒草地禾草内生细菌B-401的鉴定及生物防治潜力评价

    Institute of Scientific and Technical Information of China (English)

    畅涛; 王涵琦; 杨成德; 王颖; 杨小利; 薛莉; 陈秀蓉; 徐长林

    2014-01-01

    To study the biological control potential of B-401 endophytic bacteria in grasses on alpine grasslands, the determination of bacteriostatic ability was carried out by dual culture against plant pathogenic fungi.B-401 inhibited the growth of Phomafoveata,Stysanus stemonitis,Colletotrichum coccodes,Fusarium avenaceum, Fusariumoxysporum,Alternaria solani,Bipolaris sorokiniana and Fusarium solani by 74.45%,71.57%, 70.05%,48.01%,56.01%,60.25%,64.65% and 45.01% respectively.The control efficacy of B-401 against potato gangrene was 52.67% when a 10 times dilution of fermentation broth was sprayed on potato tubers dur-ing the storage-period.The concentration of IAA secreted by B-401 in King’s medium containing 100 mg/L tryptophan was 3.42 mg/L,and the concentration in the medium without tryptophan was 2.60 mg/L.B-401 possessed the capacity for nitrogen fixation but it did not have the capacity for phosphate solubilization.Based on morphology and physiological and biochemical characters,combined with 16S rDNA gene sequence analysis, B-401 was indentified as Bacillus atrophaeus.%为了明确高寒草地禾草内生细菌B-401的生物防治潜力,采用平板对峙法测定了其抑菌能力。抑菌谱表明, B-401对马铃薯坏疽病菌、马铃薯褐腐病菌、马铃薯炭疽病菌、马铃薯枯萎病菌、马铃薯干腐病菌、番茄早疫病菌、小麦根腐病菌、孜然根腐病菌和黄瓜枯萎病菌的抑制率分别达74.45%,71.57%,70.05%,48.01%,56.01%,60.25%,64.65%,45.01%和31.79%。在贮藏库中进行10倍液喷雾,对马铃薯坏疽病的防效达52.67%。生物学功能测定表明,该菌株在含有色氨酸和不含色氨酸的 King培养基中分泌 IAA分别为3.42和2.60 mg/L,且具有固氮能力,无溶磷能力。对B-401通过形态特征和生理生化测定,结合16S rDNA序列同源性分析,鉴定其为萎缩芽孢杆菌(Bacillusatrophaeus)。

  18. 肝素抗凝血浆与血清中微量元素测定的对比分析%Comparative Analysis of the Determination of Trace Elements in the Plasma and Serum of Heparin

    Institute of Scientific and Technical Information of China (English)

    张巧安

    2016-01-01

    目的:研究肝素抗凝血浆代替血清进行微量元素测定的可行性。方法选择2014年6月在汉川市人民医院接受生化检验的272例患者,采用Beckman DXC800型全自动生化分析仪,同时检测患者的肝素抗血浆和血清中锌(Zn)、铁(Fe)、铜(Cu)3种微量元素的浓度。结果肝素抗凝血浆锌、铁与血清中的锌、铁离子浓度比较无显著差异,差异无统计学意义( P>0.05);血浆中铜离子的浓度明显低于血清中铜离子的检测值,差异有统计学意义(P<0.05),但通过回归方程可以校正。结论肝素抗凝检测方法能够有效避开血液的凝固过程,在短时间内分离标本并检测,从而减少放置误差。但肝素抗凝血浆铜的检验还应进行回归校正。%Objective To study the feasibility of heparin anticoagulant plasma instead of serum for the determination of trace elements.Methods 272 patients who were accepted biochemical test in our hospital from June, 2014.and they were treated by Beckman DXC800 automatic biochemical analyzer.Then, the concentration of heparin in plasma and a variety of serum trace elements in patients were simultaneous detected.Results There was no significant difference in the concentration of zinc and iron in the plasma and serum of heparin, and the difference had not statistically significant (P>0.05); In addition, the concentration of copper ion in plasma was effectively lower than that in serum; Through regression analysis, the low levels of copper in the plasma may be caused by the release of blood during the coagulation of platelets, the difference had statistically significant ( P<0.05 ) .Conclusion The method of heparin anticoagulation can effectively avoid the coagulation of blood, and can be used to separate the samples in a short time, so as to reduce the dissolution and reduce the error.However, the test of heparin anticoagulant plasma copper should be carried out by regression.

  19. Scientific Analysis Cover Sheet for Radionuclide Screening

    Energy Technology Data Exchange (ETDEWEB)

    G. Ragan

    2002-08-09

    pressurized water reactor (PWR) fuel, U.S. Department of Energy (DOE) spent nuclear fuel (DSNF), and high-level waste (HLW). Average and outlying (high burnup, high initial enrichment, low age, or otherwise exceptional) forms of each waste-form type are considered. This analysis has been prepared in accordance with a technical work plan (BSC 2002c). In a review of Revision 00 of this radionuclide screening analysis, the NRC found that ''processes that affect transport in the biosphere, such as uptake by plants and bioaccumulation are not accounted for'' and that ''the direct exposure pathway is not accounted for'' (Beckman 2001, Section 5.3.2.1). The NRC also found that the solubility and sorption classes were too broadly defined, noting, for example, that Se is in the same solubility and sorptivity groups as Np and U, yet is ''more soluble than Np and U by several orders of magnitude'' (Beckman 2001, Section 5.3.2.1). This revision seeks to build upon the strengths of the earlier screening method while responding to the specific concerns raised by the NRC and other reviewers. In place of simple inhalation and ingestion dose conversion factors, the revised radionuclide screening uses screening factors that also take into account soil accumulation, uptake by plants, exposure to contaminated ground, and other features of the biosphere that were neglected in the previous screening. Whereas the previous screening analysis allowed only two solubility classes (soluble and insoluble), the revised screening introduces an intermediate solubility class to better segregate the radionuclides into transport groups.

  20. Influences of Different Instrument Standard Fluid hCG Molecular Variant On hCG Detection Results%不同的仪器标准液hCG分子变异体对hCG结果的影响

    Institute of Scientific and Technical Information of China (English)

    张忠源; 胡望平; 池细俤; 叶桂云; 胡盈莹; 闫炳龙; 张永平

    2011-01-01

    Objective To explore the rusults of human chorionicvilli gonadotropin (hCG) in serum (standard solution) with different detection system, different hospitals in Fujian Province and study their possible relationship with hCG relative molecular variants. Methods Beckman Access and Tosh AIA - 1800 immune machine detection system were used respectively to test the different concentrations of quality control things and the hCG of 44 patients. The hCG quality control data were collected from 2007 to 2009 in Fujian Province. And then literature to analysis the hCG molecular variants detection data were collected. Results Even with the same quality control things, the same serum and the same hCG relative molecular variants displayed different test results in different instrument detection system with a significant difference. The linear equation of Beckman ACCESS and Tosh AIA - 1800 was Y = 1. 3336 X - 70.199. The result of clinical serum hCG, quality control things in Tosh AIA - 1800 was 133. 8% , 137. 2% , and in Abbott was 132% . These two results were higher than those of the Beckmen ACCESS which displayed 100% . Conclusion The hCG molecular variants with different quality control things infulence the hCG detection results. Because of the hCG molecular variants, different instruments will display different hCG detection reuslts.%目的 探讨血清(标准物)中人绒毛膜促性腺激素(hCG) 在不同的检测系统、福建省各医院的测定结果,及其与hCG相关分子变异体可能的关系.方法 分别应用贝克曼Access、日本东曹(Tosh AIA-1800),在此两个免疫仪器检测系统检测不同浓度的质控物和44例患者血清的hCG;并收集福建省2007~2009年hCG质控数据、再收集文献hCG相关分子变异体检测数据进行分析.结果 同一质控物、同一血清、同一hCG相关分子变异体对不同的仪器检测系统,显示不同的测定结果,有显著差异.贝克曼Access与Tosh AIA-1800线性方程为Y=1

  1. A Retrospective Analysis of Urine Drugs of Abuse Immunoassay True Positive Rates at a National Reference Laboratory.

    Science.gov (United States)

    Johnson-Davis, Kamisha L; Sadler, Aaron J; Genzen, Jonathan R

    2016-03-01

    Urine drug screens are commonly performed to identify drug use or monitor adherence to drug therapy. The purpose of this retrospective study was to evaluate the true positive and false positive rates of one of our in-house urine drug screen panels. The urine drugs of abuse panel studied consists of screening by immunoassay then positive immunoassay results were confirmed by mass spectrometry. Reagents from Syva and Microgenics were used for the immunoassay screen. The screen was performed on a Beckman AU5810 random access automated clinical analyzer. The percent of true positives for each immunoassay was determined. Agreement with previously validated GC-MS or LC-MS-MS confirmatory methods was also evaluated. There were 8,825 de-identified screening results for each of the drugs in the panel, except for alcohol (N = 2,296). The percent of samples that screened positive were: 10.0% for amphetamine/methamphetamine/3,4-methylenedioxy-methamphetamine (MDMA), 12.8% for benzodiazepines, 43.7% for opiates (including oxycodone) and 20.3% for tetrahydrocannabinol (THC). The false positive rate for amphetamine/methamphetamine was ∼14%, ∼34% for opiates (excluding oxycodone), 25% for propoxyphene and 100% for phencyclidine and MDMA immunoassays. Based on the results from this retrospective study, the true positive rate for THC drug use among adults were similar to the rate of illicit drug use in young adults from the 2013 National Survey; however, our positivity rate for cocaine was higher than the National Survey. PMID:26668238

  2. Determination of standard enthalpies of formation and lattice energies of ammonium chloride and ammonium sulfate%氯化铵和硫酸铵生成焓与晶格能测定

    Institute of Scientific and Technical Information of China (English)

    杨春; 成文玉; 王庆伦; 刘晓莉; 王桂香; 杨津

    2013-01-01

    A new inorganic chemistry experiment of “Determination of standard enthalpies of formation and lattice energies of ammonium chloride and ammonium sulfate” has been designed . To reduce the measurement error , temperature was measured by digital Beckman thermometer and the experimental data were passed to computer for drawing and linear regress by means of software Origin . As a result , the mass of ammonium chloride used for measurement of enthalpy of dissolution decreased to about 40% .The designing experiment on ammonium sulfate can cultivate the integrated ability of students .Calculations of the lattice energies of ammonium chloride and ammonium sulfate based on their enthalpies of dissolution are introduced ,which can guide the students to research the relationship between the ionic crystal structures and physicochemical properties .%设计改进了一个新的无机化学实验“氯化铵和硫酸铵生成焓与晶格能的测定”。用数字贝克曼温度计代替水银温度计以减小测量的误差;用作图法对温度-时间曲线进行校正,提高了温度测量的准确度;在测定N H4 C1溶解焓的实验中使N H4 C1的用量减少为原来的40%,硫酸铵的设计性实验可以培养学生的综合能力,所得硫酸铵溶液可用作制备硫酸亚铁铵的原料;通过溶解热求晶格能,引导学生探究离子晶体结构与物性间的关系。

  3. Translation, modification and validation of the Chinese version of a knowledge assessment instrument regarding pressure ulcer prevention

    Institute of Scientific and Technical Information of China (English)

    Ming Liu; Hao-Bin Yuan; Wei-Ju Chen; Chiuyin Poon; Meihua Hsu; Bo Zhang

    2016-01-01

    Objective: This study sought to translate, modify and validate an instrument developed by Beckman and colleagues to assess the knowledge of clinical nurses regarding pressure ulcers. Methods: A methodological study design was used. The instrument was translated into Chinese and back-translated into English. A six-expert panel was invited to evaluate the content validity, and a pilot test was subsequently performed on the test-retest stability of the translated instrument. A convenience sampling method was used to recruit 240 nurses from a university teaching hospital in the Mainland of China. In total, 186 valid questionnaires were collected with a 77.5%valid return rate. The validity of the multiple-choice test items (item difficulties and discriminating indices) and internal consistency reli-ability were evaluated. Results: The translated and modified instrument demonstrated acceptable psychometric properties, as follows: (1) the overall content validity index (CVI) was 0.91, (2) the overall test-retest reliability was 0.826, (3) the item difficulty indices were between 0.46 and 0.93, (4) the overall values for discrimination were 0.28e0.55, and (5) the Cronbach's a for the internal consistency were 0.792 for the overall in-strument and 0.426e0.804 for the sub-themes. Conclusions: This study represents the first trial to translate and modify an existing instrument that measures the knowledge of pressure ulcers in a Chinese Mainland sample. The instrument demonstrated acceptable psychometric properties and could be applied in cross-cultural nursing practices, including nursing education, research and practice, to evaluate knowledge about pressure ulcer prevention.

  4. A Centrifuge-Based Technique for Dry Extraction of Air for Ice Core Studies of Carbon Dioxide.

    Science.gov (United States)

    Grachev, A. M.; Brook, E. J.

    2008-12-01

    High resolution CO2 data from the Law Dome ice core document an abrupt ~10 ppm drop in CO2 at about 1600 AD (MacFarling Meure et al., Geophys. Res Lett., v. 33, L14810), which has been attributed to changes in human activities. CO2 measurements in ice cores are difficult, however, making verification of this feature an important task. We are undertaking a high-resolution study of CO2 between 1400 and 1800 AD in the WAIS Divide (Antarctica) ice core with a new dry extraction technique. The need for a dry extraction technique as opposed to a melt-refreeze technique in studies of CO2 from ice cores arises because of the well-documented artifacts in CO2 imposed by the presence of liquid water. Three dry-extraction methods have been employed by previous workers to measure CO2: needle-crushing method, ball-bearings method, and cheese-grater method (B. Stauffer, in: Encyclopedia of Quaternary Science, p. 1181, Elsevier 2007). Each has limitations, and we propose a simpler dry extraction technique, based on a large-capacity refrigerated centrifuge (the "centrifuge technique"), which eliminates the need to employ cryogenic temperatures to collect extracted gas and is more compatible with high sample throughput. The technique is now being tested on ~25-gram WAIS Divide samples in conjunction with CO2 measurements with a gas chromatograph. The technique employs a Beckman J- 6B centrifuge, in which evacuated stainless steel flask is placed: the flask has a weight inside positioned directly over a tall-standing piece of ice whose cross-section is small compared to that of the flask. Upon acceleration to 3000 rpm the weight moves down and presses the ice sample into a thin tablet covering flask's bottom, yielding the air extraction efficiency of ~80%. Preliminary tests suggest that precision and accuracy can be achieved at the level of ~1 ppm once the system is fine-tuned.

  5. A new concept for combisystems characterization. The FSC method

    Energy Technology Data Exchange (ETDEWEB)

    Letz, Thomas [INES - Education, Parc Technologique de Savoie Technolac, 50 avenue du Leman BP 258, F-73 375 Le Bourget du Lac Cedex (France); Bales, Chris [Solar Energy Research Center SERC, School of Industrial Technology and Management, Hoegskolan Dalarna, S-78188 Borlaenge (Sweden); Perers, Bengt [DTU Civil Engineering, Technical University of Denmark, Brovej, Building 118, DK-2800 Kgs. Lyngby (Denmark)

    2009-09-15

    Solar combisystems are relatively complex systems with many different components and operational parameters. Before the beginning of IEA-SHC Task 26 ('solar combisystems'), no method was available with which they could be compared. The well known 'f-chart' method was introduced by Duffie and Beckman already in the seventies, but was only useful for dimensioning generic combisystems, with a defined hydraulic scheme. It didn't give a method to compare different designs. The objective of this work was to develop a simple tool for characterizing the performance of these systems. The method used was to analyse the comprehensive simulation results of Task 26 and to look for relationships between the key external factors of climate and load, and the system performance. The result is a new and simple methodology for characterization of solar combisystems, called the fractional solar consumption (FSC) method. FSC is a dimensionless quantity, which takes simultaneously into account the climate, the space heating and domestic hot water loads, the collector size, its orientation and tilt angle, but which does not depend on the studied system design. The study shows that fractional energy savings, with and without parasitic energy included, can be expressed as a quadratic function of FSC. The relationship was shown to be valid for a wide range of conditions, but to be limited for certain parameters such as collector orientation and hot water load. The method has been used to create a nomogram and the computer design tool CombiSun. (author)

  6. Frameshift Deletion by Sulfolobus solfataricus P2 DNA Polymerase Dpo4 T239W Is Selective for Purines and Involves Normal Conformational Change Followed by Slow Phosphodiester Bond Formation*

    Science.gov (United States)

    Zhang, Huidong; Beckman, Jeff W.; Guengerich, F. Peter

    2009-01-01

    The human DNA polymerase κ homolog Sulfolobus solfataricus DNA polymerase IV (Dpo4) produces “−1” frameshift deletions while copying unmodified DNA and, more frequently, when bypassing DNA adducts. As judged by steady-state kinetics and mass spectrometry, bypass of purine template bases to produce these deletions occurred rarely but with 10-fold higher frequency than with pyrimidines. The DNA adduct 1,N2-etheno-2′-deoxyguanosine, with a larger stacking surface than canonical purines, showed the highest frequency of formation of −1 frameshift deletions. Dpo4 T239W, a mutant we had previously shown to produce fluorescence changes attributed to conformational change following dNTP binding opposite cognate bases (Beckman, J. W., Wang, Q., and Guengerich, F. P. (2008) J. Biol. Chem. 283, 36711–36723), reported similar conformational changes when the incoming dNTP complemented the base following a templating purine base or bulky adduct (i.e. the “+1” base). However, in all mispairing cases, phosphodiester bond formation was inefficient. The frequency of −1 frameshift events and the associated conformational changes were not dependent on the context of the remainder of the sequence. Collectively, our results support a mechanism for −1 frameshift deletions by Dpo4 that involves formation of active complexes via a favorable conformational change that skips the templating base, without causing slippage or flipping out of the base, to incorporate a complementary residue opposite the +1 base, in a mechanism previously termed “dNTP-stabilized incorporation.” The driving force is attributed to be the stacking potential between the templating base and the incoming dNTP base. PMID:19837980

  7. Frameshift deletion by Sulfolobus solfataricus P2 DNA polymerase Dpo4 T239W is selective for purines and involves normal conformational change followed by slow phosphodiester bond formation.

    Science.gov (United States)

    Zhang, Huidong; Beckman, Jeff W; Guengerich, F Peter

    2009-12-11

    The human DNA polymerase kappa homolog Sulfolobus solfataricus DNA polymerase IV (Dpo4) produces "-1" frameshift deletions while copying unmodified DNA and, more frequently, when bypassing DNA adducts. As judged by steady-state kinetics and mass spectrometry, bypass of purine template bases to produce these deletions occurred rarely but with 10-fold higher frequency than with pyrimidines. The DNA adduct 1,N(2)-etheno-2'-deoxyguanosine, with a larger stacking surface than canonical purines, showed the highest frequency of formation of -1 frameshift deletions. Dpo4 T239W, a mutant we had previously shown to produce fluorescence changes attributed to conformational change following dNTP binding opposite cognate bases (Beckman, J. W., Wang, Q., and Guengerich, F. P. (2008) J. Biol. Chem. 283, 36711-36723), reported similar conformational changes when the incoming dNTP complemented the base following a templating purine base or bulky adduct (i.e. the "+1" base). However, in all mispairing cases, phosphodiester bond formation was inefficient. The frequency of -1 frameshift events and the associated conformational changes were not dependent on the context of the remainder of the sequence. Collectively, our results support a mechanism for -1 frameshift deletions by Dpo4 that involves formation of active complexes via a favorable conformational change that skips the templating base, without causing slippage or flipping out of the base, to incorporate a complementary residue opposite the +1 base, in a mechanism previously termed "dNTP-stabilized incorporation." The driving force is attributed to be the stacking potential between the templating base and the incoming dNTP base. PMID:19837980

  8. Characterization of Fricke dosimeter developed at Centro Regional de Ciencias Nucleares, Recife, PE, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Vivianne L.B.; Cunha, Manuela S.; Santos, Carla D.A.; Figueiredo, Marcela D.C.; Rodrigues, Kelia R.G.; Melo, Roberto T.; Hazin, Clovis A., E-mail: vlsouza@cnen.gov.b, E-mail: manusc06@hotmail.co, E-mail: daysebutterfly@hotmail.co, E-mail: keliareja@hotmail.co, E-mail: rtmelo@cnen.gov.b, E-mail: chazin@cnen.gov.b [Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil)

    2009-07-01

    Fricke Xylenol Gel (FXG) is a modified Fricke dosimeter system that was tested at the Centro Regional de Ciencias Nucleares (CRCN/CNEN), in Recife, Brazil. The Fricke dosimeter system has been used in our laboratory successfully. We decided to test the FXG in our laboratory because this dosimetric system presents some advantages in relation to the Fricke dosimeter, such as: easy preparation, more sensitivity for doses between 5 - 30 Gy, good reproducibility, tissue equivalence and multidimensional dose information. The dosimetric solution was synthesized with 0.392 g of ferrous ammonium sulphate, 0.0060 g of sodium chloride, 0.0076 g of xylenol orange, 5.0 g of gelatine (270 bloom porcine) and 300 mul of sulphuric acid in 100 ml of water. First, the water-gelatin mixture was heated and continuously stirred with a magnetic stirrer while the others reagents were added and the temperature was raised to 45 deg C. Once the solution ready it was transferred to acrylic cuvettes and kept in dark under refrigeration at 5 deg C during 12 h. Characteristics such as absorption spectrum, stability and linearity were evaluated by spectrophotometry (Beckman DU-640 UV-visible spectrophotometer). The developed material presented a good performance: ferric ion concentration at 589 nm, after irradiation; linear dose-response curve between 5 and 30 Gy; and saturation region for doses higher than 30 Gy. One disadvantage of the FXG system is its poor storage stability, because the non-irradiated samples suffer a selfdegradation and this could be a limitation for the use of FXG system. (author)

  9. Enhanced radiation response in radioresistant MCF-7 cells by targeting peroxiredoxin II

    Directory of Open Access Journals (Sweden)

    Diaz AJG

    2013-10-01

    Full Text Available Anthony Joseph Gomez Diaz,1 Daniel Tamae,2 Yun Yen,3 JianJian Li,4 Tieli Wang1 1Department of Chemistry and Biochemistry, California State University at Dominguez Hills, Carson, CA, 2Center of Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania, Philadelphia, PA, 3Department of Clinical and Molecular Pharmacology, Beckman Research Institute of City of Hope National Medical Center, Duarte, CA, 4Department of Radiation Oncology, University of California Davis, Sacramento, CA, USA Abstract: In our previous study, we identified that a protein target, peroxiredoxin II (PrxII, is overexpressed in radioresistant MCF+FIR3 breast-cancer cells and found that its expression and function is associated with breast-cancer radiation sensitivity or resistance. Small interference RNA (siRNA targeting PrxII gene expression was able to sensitize MCF+FIR3 radioresistant breast-cancer cells to ionizing radiation. The major focus of this work was to investigate how the radiation response of MCF+FIR3 radioresistant cells was affected by the siRNA that inhibits PrxII gene expression. Our results, presented here, show that silencing PrxII gene expression increased cellular toxicity by altering cellular thiol status, inhibiting Ca2+ efflux from the cells, and perturbing the intracellular Ca2+ homeostasis. By combining radiotherapy and siRNA technology, we hope to develop new therapeutic strategies that may have potential to enhance the efficacy of chemotherapeutic agents due to this technology's property of targeting to specific cancer-related genes. Keywords: siRNA, PrxII, radiation resistance, Ca2+, MCF+FIR3

  10. Screening for fetal chromosome abnormalities during the second trimester

    International Nuclear Information System (INIS)

    Objective: To develop a pre -natal screening program for fetal chromosome abnormalities based on risk values calculated from maternal serum markers levels during the second trimester. Methods: Serum levels of AFP, β-HCG, uE3 were determined with CLIA in 1048 pregnant women during 14-21w gestation period and the results were analyzed with a specific software (screening program for Down' s syndrome developed by Beckman) for the risk rate. In those women defined as being of high risk rate, cells from amniotic fluid or umbilical cord blood were studied for karyotype analysis. Results: Of these 1048 women, 77 were designated as being of high risk rate for several chromosome abnormalities i.e. Down's syndrome, open spina bifida and trisomy -18 syndrome (overall positive rate 7.3%). Further fetal chromosome study in 31 of them revealed three proven cases of abnormality. Another cord blood study was performed in a calculated low risk rate case but with abnormal sonographic finding at 31 w gestation and proved to be abnormal (software study false negative). The remaining 46 high risk rate cases either refused future study (n=35) or were lost for follow-up (n=11). Fortunately, all the 35 women refused further study gave birth to normal babies without any chromosome abnormalities discovered on peripheral blood study. Besides, in a trial study, five high risk rate women were again evaluated a few weeks later but with tremendous difference between the results. Conclusion: The present program proves to be clinically useful but needs further study and revision. Many factors may influence the result of the analysis and the duration of gestation period in weeks should be as accurate as possible. At present, in order to avoid getting false negatives, we don't advise a second check in 'high risk' cases. (authors)

  11. [Effects of ruta SSP on the activity of the smooth gastrointestinal muscle isolated of rat].

    Science.gov (United States)

    Grigorjev, Carlota A; Brizuela, Nilda Y

    2010-01-01

    Ruta graveolens L. and Ruta chalepensis L. are plants used in folkloric medicine as antispasmodics, digestive and for intestinal gases. Animals used as experimental model were rats of the Wistar line, adult females, clinically healthy and with a weight average of 250 grams. We used strips of stomach and duodenum. Each one of the segments mounted on two stirrups in a bath of organ isolated with Ringer-lactate solution, at 37° C , pH: 7.3-7.4, and bubbled with 95% O2, 5% CO2. One of the stirrups was connected vertically to the bottom of the bath and the other to a transducer of tension connected to a Beckman polygraph. We applied 500 mgs of basal tension. After the stabilization, the ethanolic extract of Ruta ssp was added in increasing doses. At 50μl/ml the tone lower 23% in small intestine and lower 27% in stomach. However at 100 μl/ml the tone lower 32% and 35% respectively. In the other parameters the amplitude decrease 50% in the stomach at dose of 5 μl/ml while in the small intestine the amplitude lower 60%. With 10 μl/ml the amplitude change in both organs ( 96% in small intestine, and 75% in stomach). The frequency changes in both organs ( 32% in small intestine, and 50% in stomach) at 10 μl/ml Rue showed decreased effects on isolated small intestine and stomach were is dose dependent, maybe we were demonstrated the effects digestive of Ruta.

  12. Measurement of isotopic uranium in water for compliance monitoring by liquid scintillation counting with alpha/beta discrimination

    International Nuclear Information System (INIS)

    A simple and inexpensive method is described for analysis of uranium (U) activity and mass in water by liquid scintillation counting using α/β discrimination. This method appears to offer a solution to the need for an inexpensive protocol for monitoring U activity and mass simultaneously and an alternative to the potential inaccuracy involved when depending on the mass-to-activity conversion factor or activity screen. U is extracted virtually quantitatively into 20 ml extractive scintillator from a 1-ell aliquot of water acidified to less than pH 2. After phase separation, the sample is counted for a 20-minute screening count with a minimum detection level of 0.27 pCi ell -1. α-particle emissions from the extracted U are counted with close to 100% efficiency with a Beckman LS6000 LL liquid scintillation counter equipped with pulse-shape discrimination electronics. Samples with activities higher than 10 pCi ell -1 are recounted for 500-1000 minutes for isotopic analysis. Isotopic analysis uses events that are automatically stored in spectral files and transferred to a computer during assay. The data can be transferred to a commercially available spreadsheet and retrieved for examination or data manipulation. Values for three readily observable spectral features can be rapidly identified by data examination and substituted into a simple formula to obtain 234U/238U ratio for most samples. U mass is calculated by substituting the isotopic ratio value into a simple equation. The utility of this method for the proposed compliance monitoring of U in public drinking water supplies was field tested with a survey of drinking water from Texas supplies that had previously been known to contain elevated levels of gross α activity. U concentrations in 32 samples from 27 drinking water supplies ranged from 0.26 to 65.5 pCi ell -1, with seven samples exceeding the proposed Maximum Contaminant Level

  13. Characterization of Fricke dosimeter developed at Centro Regional de Ciencias Nucleares, Recife, PE, Brazil

    International Nuclear Information System (INIS)

    Fricke Xylenol Gel (FXG) is a modified Fricke dosimeter system that was tested at the Centro Regional de Ciencias Nucleares (CRCN/CNEN), in Recife, Brazil. The Fricke dosimeter system has been used in our laboratory successfully. We decided to test the FXG in our laboratory because this dosimetric system presents some advantages in relation to the Fricke dosimeter, such as: easy preparation, more sensitivity for doses between 5 - 30 Gy, good reproducibility, tissue equivalence and multidimensional dose information. The dosimetric solution was synthesized with 0.392 g of ferrous ammonium sulphate, 0.0060 g of sodium chloride, 0.0076 g of xylenol orange, 5.0 g of gelatine (270 bloom porcine) and 300 μl of sulphuric acid in 100 ml of water. First, the water-gelatin mixture was heated and continuously stirred with a magnetic stirrer while the others reagents were added and the temperature was raised to 45 deg C. Once the solution ready it was transferred to acrylic cuvettes and kept in dark under refrigeration at 5 deg C during 12 h. Characteristics such as absorption spectrum, stability and linearity were evaluated by spectrophotometry (Beckman DU-640 UV-visible spectrophotometer). The developed material presented a good performance: ferric ion concentration at 589 nm, after irradiation; linear dose-response curve between 5 and 30 Gy; and saturation region for doses higher than 30 Gy. One disadvantage of the FXG system is its poor storage stability, because the non-irradiated samples suffer a selfdegradation and this could be a limitation for the use of FXG system. (author)

  14. Determination of Ondansetron in Human Plasma by RP-HPLC%昂丹司琼人体血药浓度的RP-HPLC测定法

    Institute of Scientific and Technical Information of China (English)

    张尊建; 邹巧根; 李伟; 燕立波; 相秉仁; 吴如金

    2002-01-01

    目的:建立人血浆中昂丹司琼的RP-HPLC测定法,用于昂丹司琼片的人体药代动力学研究.方法:血浆样品加乙腈脱蛋白后,离心,取上清液,挥干,用少量流动相溶解,供进样分析.采用Alltima C18柱(4.6 mm×250 mm,5 μm),预柱:Beckman-ultrasphere ODS(4.6 mm×45 mm,5 μm),以醋酸钠缓冲液(pH=4.2)-乙腈(75∶ 25)为流动相,流速:1 mL*min-1,于310 nm波长处检测,灵敏度:0.000 1 AUFS. 测定了10名健康志愿者单剂量口服昂丹司琼8 mg后的血药浓度-时间过程.结果:最低检测限达0.5 ng*mL-1,线性范围为1~80 ng*mL-1(r=0.992 2),日内和日间精密度均小于10%,平均回收率为88.7%~98.2%,符合生物样品分析要求.结论:本法操作简单,准确,灵敏度高,可用于昂丹司琼片的药代动力学研究.

  15. 上海地区血液分析仪准确性的研究和探讨%Study on the accuracy of automated hematology analyzers in Shanghai

    Institute of Scientific and Technical Information of China (English)

    胡晓波; 李泳; 吴驾浦; 黄永华; 宋颖; 许蕾; 金大鸣; 张锦锋

    2001-01-01

    目的探讨上海地区不同品牌、型号血液自动分析仪检测参数结果准确度,证明仪器间差异是否有显著性.方法采用同一份抗凝全血由上海市114家单位统一测定血液参数,计算测定结果的变异系数(CV)和极差(%).结果不同仪器组(Sysmex组、Beckman-Coulter组和Abbott组)测定结果最大变异系数比较,红细胞计数(RBC)、血红蛋白量(HGB)、血细胞比容(HCT)、白细胞计数(WBC)和血小板计数(PLT)分别为3.2%,3.8%,3.6%,9.3%和10.8%.不同仪器组测定结果之间最大差异比较,RBC、HGB、HCT、MCV、WBC和PLT分别为0.74%、1.65%、5.45%、6.42%、7.06%和18.55%.结论开展深入实验室质量管理工作,可使血液分析仪检测质量的水平可缩小不同仪器检测结果之间差异,使得同一血样在不同实验室的测定结果趋向一致.

  16. The effect of leukocyte proof filter on coagulation factor and plasma protein in fresh frozen plasma%白细胞滤器对新鲜冰冻血浆凝血因子及血浆蛋白的影响

    Institute of Scientific and Technical Information of China (English)

    蔡杰; 胡俊妍; 陈映娥; 杨建鑫; 程本坤

    2006-01-01

    目的探讨经白细胞过滤器过滤后的新鲜冰冻血浆(Fresh frozen plasma, FFP)中凝血因子的生物活性及血浆蛋白含量变化. 方法随机抽取30份新鲜冰冻血浆, 置于37℃水浴融化,在超净工作台内留取过滤前后血浆样本各2ml, 使用Sysmex1500全自动血凝仪测定凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原(Fbg)、凝血因子Ⅶ (FⅦ:C)、凝血因子Ⅷ (FⅧ:C)、凝血因子Ⅸ(FⅨ:C)水平;用BECKMAN LX20全自动生化分析仪对TP、ALB进行检测. 结果过滤前后的新鲜冰冻血浆TT、 Fbg、FⅦ、FⅨ、TP、ALB水平的差异均无显著性(P>0.05).APTT、PT、FⅧ过滤前后的差异有显著性(P<0.05), 但仍在参考值范围内. 结论过滤前后新鲜冰冻血浆中凝血因子的活性差异变化及血浆蛋白的含量在参考值范围内, 适用于临床治疗.

  17. Power-free chip enzyme immunoassay for detection of prostate specific antigen (PSA) in serum.

    Science.gov (United States)

    Adel Ahmed, Heba; Azzazy, Hassan M E

    2013-11-15

    A power-free, portable "Chip EIA" was designed to render the popular Enzyme Linked Immunosorbent Assay (ELISA) more suitable for point-of-care testing. A number of microfluidic platforms have enabled miniaturization of the conventional microtitre plate ELISA, however, they require external pumping systems, valves, and electric power supply. The Chip EIA platform has eliminated the need for pumps and valves through utilizing a simple permanent magnet and magnetic nanoparticles. The magnetic nanoparticles act as solid support to capture the target and are then moved through chambers harboring different reagents necessary to perform a sandwich ELISA. The use of magnetic nanoparticles increases the volume-to-surface ratio reducing the assay time to 30 min. Changing the color of horseradish peroxidase (HRP) substrate to green indicates a positive result. In addition, a quantitative read-out was obtained through the use of cellphone camera imaging and analyzing the images using Matlab®. Cell phones, including smart ones, are readily available almost everywhere. The Chip EIA device was used to assay total prostate specific antigen (tPSA) in 19 serum samples. The PSA Chip EIA was tested for accuracy, precision, repeatability, and the results were correlated to the commercial Beckman Colter, Hybritech immunoassay® for determination of tPSA in serum samples with a Pearson correlation coefficient (R(2)=0.96). The lower detection limit of the PSA Chip EIA was 3.2 ng/mL. The assay has 88.9% recovery and good reproducibility (% CV of 6.5). We conclude that the developed Chip EIA can be used for detection of protein biomarkers in biological specimens.

  18. Rapid detection of acute kidney injury by urinary neutrophil gelatinase-associated lipocalin after cardiopulmonary bypass surgery

    International Nuclear Information System (INIS)

    Objective: To determine the accuracy of neutrophil gelatinase-associated lipocalin (NGAL) in early detection of acute kidney injury (AKI) after cardiopulmonary bypass (CPB) surgery by comparing with serum creatinine. Study Design: Descriptive study. Place and Duration of Study: Department of Chemical Pathology and Endocrinology, AFIP in collaboration with AFIC/ NIHD, Rawalpindi, from April to December 2011. Methodology: Eighty eight patients undergoing CPB surgery in AFIC/NIHD were included by consecutive sampling. Blood samples of subjects for serum creatinine analysis were drawn pre-operatively, 4 h, 24 h and 48 h after CPB surgery. Spot urine samples for NGAL were collected at 4 h after CPB surgery. Urine samples were analyzed on Abbott ARCHITECT i2000SR analyzer whereas serum creatinine samples were measured on Beckman UniCel DxC 600 Synchron Clinical System. Results: Out of 88 patients, 11 (13%) cases developed AKI 4 h postoperatively. Urinary NGAL increased markedly at 4 h postoperatively as compared to serum creatinine which showed rise at 24 - 48 h after cardiac surgery. Analysis of urine NGAL at a cutoff value of 87 ng/ml showed area under the curve of 0.91 [95% confidence interval (CI) 0.83 - 0.96] with sensitivity of 90.9% (95% CI 58.7 - 98.5) and specificity of 98.7% (95% CI 92.9-99.8). There was a positive correlation of 4 h urine NGAL and serum delta creatinine at 48 h, which was statistically significant (rs = 0.33, p = 0.001). Conclusion: The study demonstrated that levels of urine NGAL in patients suffering from AKI increased significantly at 4 h as compared to serum creatinine levels. Urine NGAL is an early predictive biomarker of AKI after CPB. (author)

  19. Comparison of urine with plasma neutrophil gelatinase-associated lipocalin in detecting acute kidney injury after cardiopulmonary bypass surgery

    International Nuclear Information System (INIS)

    Objective: To compare the accuracy of urine with plasma neutrophil gelatinase-associated lipocalin (NGAL) in early detection of acute kidney injury (AKI) following cardiopulmonary bypass (CPB) surgery. Study Design: A prospective cohort study. Place and duration of study: Department of Chemical Pathology and Endocrinology, AFIP from December 2011 to July 2012. Patients and Methods: Ninety three adult patients planned for CPB surgery in AFIC/NIHD were consecutively included. Blood for serum creatinine were collected preoperatively, 4, 24 and 48 hours (h) after CPB surgery. Blood and urine samples for NGAL analysis were collected only at 4 h. Serum creatinine, plasma and urine NGAL samples were analyzed on UniCel at the rate DxC 600 (Beckman), TRIAGE meter pro (Biosite) and ARCHITECT i2000SR analyzer (Abbott) respectively. Results: Out of 93 patients undergoing CPB surgery, 12 (13%) developed AKI. AKI patients had significantly higher median interquartile range (IQR) urine NGAL of 180 ng/ml (105-277 ng/ml) as compared to control of 6 ng/ml (2-15 ng/ml) and median plasma NGAL of 170 ng/ml (126-274 ng/ml) as compared to control of 75 ng/ml (61-131 ng/ml). The patients had increased urine vs plasma NGAL area under curve (AUC) ( 0.91 vs 0.70 (p = <0.001)), better sensitivity (91% vs 82%) and specificity (98% vs 65%). Conclusion: Plasma and urine NGAL values increased significantly in AKI patients as compared to serum creatinine values. Urine in comparison to plasma NGAL revealed more sensitivity and specificity in detecting AKI following CPB surgery. (author)

  20. Annual symposium on Frontiers in Science

    Energy Technology Data Exchange (ETDEWEB)

    Metzger, N.; Fulton, K.R.

    1998-12-31

    This final report summarizes activities conducted for the National Academy of Sciences' Annual Symposium on Frontiers of Science with support from the US Department of Energy for the period July 1, 1993 through May 31, 1998. During the report period, five Frontiers of Science symposia were held at the Arnold and Mabel Beckman Center of the National Academies of Sciences and Engineering. For each Symposium, an organizing committee appointed by the NAS President selected and planned the eight sessions for the Symposium and identified general participants for invitation by the NAS President. These Symposia accomplished their goal of bringing together outstanding younger (age 45 or less) scientists to hear presentations in disciplines outside their own and to discuss exciting advances and opportunities in their fields in a format that encourages, and allows adequate time for, informal one-on-one discussions among participants. Of the 458 younger scientists who participated, over a quarter (124) were women. Participant lists for all symposia (1993--1997) are attached. The scientific participants were leaders in basic research from academic, industrial, and federal laboratories in such disciplines as astronomy, astrophysics, atmospheric science, biochemistry, cell biology, chemistry, computer science, earth sciences, engineering, genetics, material sciences, mathematics, microbiology, neuroscience, physics, and physiology. For each symposia, the 24 speakers and discussants on the program were urged to focus their presentations on current cutting-edge research in their field for a scientifically sophisticated but non-specialist audience, and to provide a sense of the experimental data--what is actually measured and seen in the various fields. They were also asked to address questions such as: What are the major research problems and unique tools in their field? What are the current limitations on advances as well as the frontiers? Speakers were asked to provide a

  1. Use of the selected overlap LIDAR experiment (SOLEX) system with the 248 nm krypton fluoride and the 355 nm neodymium:yttrium aluminum garnet lasers for the calibration of LIDAR systems for water vapor determination

    Science.gov (United States)

    Mensah, Francis Emmanuel Tofodji

    Water vapor is one of the most important atmospheric variables that play a key role in air quality, global warming, climate change and hurricane formation. In this dissertation, use was made of two laser systems, the 248-nm KrF laser and the 355 nm Nd-YAG laser, with the use of Raman scattering to measure water vapor in the atmosphere. These two systems have been calibrated more accurately, using the LIDAR approach named SOLEX (Selected Overlap LIDAR Experiment). All the experiments were carried out at the Howard University Beltsville campus located on a 107 acre research site, at Beltsville, MD, 15 miles from downtown Washington DC, near the National Agricultural Research Center (NARC), and the NASA Goddard Space Flight Center (GSFC). The geographical coordinates are: 39°04.01'N latitude, and 76°52.31'W longitude. The receiver system used during these experiments is a 30" (76.2 cm), f/ 9 Cassegranian telescope, while the detector system uses a prism spectrometer (Beckman), with a 2-meter, double-fold optical path and a variable slit width is placed at the image plane of the telescope. With the use of the SOLEX system, this dissertation provides an accurate calibration of the two LIDAR Systems for water vapor measurement in the troposphere at the following ranges: 83.7 ft, 600 ft, 800 ft, 1000 ft and 1080 ft. Data analysis shows a pretty high sensitivity of the LIDAR system for water vapor measurement and the efficiency of the SOLEX method.

  2. Use of an external source (60Co) for 32P detection efficiency determination by the Cerenkov effect, in soil extracts

    International Nuclear Information System (INIS)

    The detection of 32P in aqueous extracts is usually made with the aid of a Geiger-Muller detector, with thin window and sample on a planchet. Presently the technique is being developed of detection of high energy beta particles emitters (32P, 42K, 86Rb) through the Cerenkov effect, using a commercial liquid scintillation system. This technique, despite being approximately 30 times more sensitive, has the inconvenience of varying the detection efficiency, mainly for color samples (soil extracts, for instance). From this stems the need for determining the detection efficiency for each sample. The internal standardization and channels ratio methods show a series of drawbacks, mainly the non-reutilization of the samples (1st method) and statistical uncertainty for low activity samples (2nd method). The elimination of these dreawbacks can be achieved through the utilization of the external standardization method. A 60Co source with 1,4 μCi activity has been adapted to the sample elevator of the detector system, and a comparison was made with the channels ratio method to evaluate the efficiency of 32P detection in soil extracts (P extraction and fractionation). The external standardization method showed to be more accurate, besides being influenced to a lesser degree by high voltage variation, sample volume and vial types. In the case of large samples, it is advisable to carry out detection in vials filled up to their full capacity; in the case of small samples, the whole volume should be transferred to the vials and completed up to 9 ml for nylon vials,10 ml for glass vials and 11 to 14 ml for polyethilene vials. On the other hand, plastic vials showed higher detection efficiency than ones. As to background radiation, the lowest rates were given by nylon vials and the highest by Beckman glass vials

  3. Growth and radiosensitivity of irradiated human glioma cell progeny

    Institute of Scientific and Technical Information of China (English)

    Chao Li; Li Li; Changshao Xu; Juying Zhou

    2008-01-01

    BACKGROUND: Progenitors of the immortalized human glioma cell line, SHG-44, are significantly less sensitive to irradiation. Two hypotheses regarding the mechanism of this effect exist: several studies have suggested that there is a subgroup with different radiosensitivities in identical cell group, and the progenitors of irradiate is a adaptive response subgroup, so its radiosensitivity is descend. A second hypothesis suggests that irradiated glioma progeny have a stronger ability to repair DNA damage. This would suggest that when progeny are continuously irradiated, resistance to irradiation-induced DNA increases, and radiosensitivity decreases.OBJECTIVE: To investigate radiosensitivity and growth features after irradiation to progeny of the human glioma cell line SHG-44.DESIGN, TIME AND SETTING: A randomized, controlled experiment, which was performed at the Department of Radiology Laboratory, the First Hospital Affiliated to Soochow University, between September 2004 and January 2006.MATERIALS: The glioma cell line SHG-44 was provided by the Institute of Neuroscience, First Affiliated Hospital of Suzhou University. Propidium iodide reagent was provided by Coulter Corporation. A linear accelerator, KD-2 type, was provided by Siemens, Germany. The flow cytometer EPICS-XL was provided by Coulter Corporation.METHODS: Brain glioma SHG-44 cells were divided into four groups: SHG-44, SHG-44-2, SHG-44-6, and SHG-44-10. The SHG-44-2, SHG-44-6, and SHG-44-10 cells were vertically irradiated with varying doses of 2,6 and 10 Gy by a linear accelerator (6 MVX). The cells were passaged for 15 generations and cultured in RPMI-1640 culture media.MAIN OUTCOME MEASURES: Community re-double time, mean lethal dose (D0), extrapolation number (N), fraction surviving fraction irradiated by 2 Gy dose (SF2), quasi-threshold dose (Dq), and cell cycle.RESULTS: The Population doubling time (PDT) of SHG-44-2, SHG-44-6, and SHG-44-10 cell groups was not significant (P=0.052). Compared to

  4. Sources of SOA gaseous precursors in contrasted urban environments: a focus on mono-aromatic compounds and intermediate volatility compounds

    Science.gov (United States)

    Salameh, Therese; Borbon, Agnès; Ait-Helal, Warda; Afif, Charbel; Sauvage, Stéphane; Locoge, Nadine; Bonneau, Stéphane; Sanchez, Olivier

    2016-04-01

    , SP95 E10, and SP98) and was used to constraint evaporative emissions in order to predict the headspace vapour composition (Harley and Coulter-Burke, 2000). Modelled and observed compositions are in good agreement (differences up to 20%). Therefore, the implemented model is a relevant tool to test the sensitivity of BTEX and other VOCs ambient composition to evaporative emissions of fuels with regards to their composition. Such analysis will be extended to other target cities and similarities/differences will be presented regarding regional characteristics. This work was supported by the Ile de France region, Life and PHOTOPAQ grant, PICS-CNRS, ENVIMED and ChArMEx. We would like to thank Laurence Dépelchin and Thierry Léonardis for technical support and AIRPARIF for providing the data. Borbon, A., et al. (2013) Emission ratios of anthropogenic VOC in northern mid-latitude megacities: observations vs. emission inventories in Los Angeles and Paris, J. Geophys. Res. 118, 2041 - 2057. Harley, R. and Coulter-Burke, S. (2000) Relating Liquid Fuel and Headspace Vapor Composition for California Reformulated Gasoline Samples Containing Ethanol, Environ. Sci. Technol. 34, 4088-4094. Ait-Helal, W.; Borbon, A.; Sauvage, S.; et al., Atmos. Chem. Phys. vol. 14 , No. 19 , p. 10439-10464

  5. The Putative Role of the Non-Gastric H+/K+-ATPase ATP12A (ATP1AL1 as Anti-Apoptotic Ion Transporter: Effect of the H+/K+ ATPase Inhibitor SCH28080 on Butyrate-Stimulated Myelomonocytic HL-60 Cells

    Directory of Open Access Journals (Sweden)

    Martin Jakab

    2014-10-01

    Full Text Available Background/Aims: The ATP12A gene codes for a non-gastric H+/K+ ATPase, which is expressed in a wide variety of tissues. The aim of this study was to test for the molecular and functional expression of the non-gastric H+/K+ ATPase ATP12A/ATP1AL1 in unstimulated and butyrate-stimulated (1 and 10 mM human myelomonocytic HL-60 cells, to unravel its potential role as putative apoptosis-counteracting ion transporter as well as to test for the effect of the H+/K+ ATPase inhibitor SCH28080 in apoptosis. Methods: Real-time reverse-transcription PCR (qRT-PCR was used for amplification and cloning of ATP12A transcripts and to assess transcriptional regulation. BCECF microfluorimetry was used to assess changes of intracellular pH (pHi after acute intracellular acid load (NH4Cl prepulsing. Mean cell volumes (MCV and MCV-recovery after osmotic cell shrinkage (Regulatory Volume Increase, RVI were assessed by Coulter counting. Flow-cytometry was used to measure MCV (Coulter principle, to assess apoptosis (phosphatidylserine exposure to the outer leaflet of the cell membrane, caspase activity, 7AAD staining and differentiation (CD86 expression. Results: We found by RT-PCR, intracellular pH measurements, MCV measurements and flow cytometry that ATP12A is expressed in human myelomonocytic HL-60 cells. Treatment of HL-60 cells with 1 mM butyrate leads to monocyte-directed differentiation whereas higher concentrations (10 mM induce apoptosis as assessed by flow-cytometric determination of CD86 expression, caspase activity, phosphatidylserine exposure on the outer leaflet of the cell membrane and MCV measurements. Transcriptional up-regulation of ATP12A and CD86 is evident in 1 mM butyrate-treated HL-60 cells. The H+/K+ ATPase inhibitor SCH28080 (100 µM diminishes K+-dependent pHi recovery after intracellular acid load and blocks RVI after osmotic cell shrinkage. After seeding, HL-60 cells increase their MCV within the first 24 h in culture, and subsequently

  6. 两种微生物学等级老龄Wistar大鼠血清生化值比较%Comparison of serum biochemistry between specific pathogen-free and coventional aged Wistar rats

    Institute of Scientific and Technical Information of China (English)

    肖育华; 詹纯列; 李建军; 武婕; 李新春; 郑文岭

    2004-01-01

    目的探讨普通级、SPF级老龄Wistar大鼠血清生化值的差异.方法用Coulter-JT全自动生化检测仪检测血清生化值.结果在老年SPF级Wistar大鼠的雌性与雄性之间,谷丙酶、总蛋白、碱性磷酸酶、总胆固醇、甘油三酯、尿素氮、肌酐、铁、磷、血糖、尿酸、低密度脂蛋白差异非常显著(P<0.01),白蛋白、乳酸脱氢酶、载脂蛋白B差异显著(P<0.05).老年SPF级雄性Wistar大鼠与老年普通级雄性Wistar大鼠间的总蛋白、白蛋白、白球比、总胆固醇、甘油三酯、血糖、载脂蛋白A1、载脂蛋白B、尿酸、高密度脂蛋白、低密度脂蛋白、谷草酶差异非常显著(P<0.01),谷丙酶、铁、镁差异显著(P<0.05).老年SPF级雌性Wistar大鼠与普通级雌性Wistar大鼠间的谷丙酶、总蛋白、白蛋白、白球比、碱性磷酸酶、甘油三酯、尿素氮、肌酐、铁、载脂蛋白A1、载脂蛋白B、高密度脂蛋白、低密度脂蛋白、胆汁酸差异非常显著(P<0.01),镁含量差异显著(P<0.05).结论微生物学等级因素对Wistar大鼠的血清生化值有影响.%Objective To investigate the differences in serum biochemistry between specific pathogen-free (SPF) and conventional aged Wistar rats. Methods Coulter-JT Analyzer was used to measure the values of serum biochemistry in the two grades of rats. Results The serum levels of alanine aminotransferase (ALT), total protein (TP), alkaline phosphatase (ALP), total cholesterol (TC), triglyceride (TG), blood urea nitrogen (BUN), creatinine, Fe, P, glucose, uric acid (UA), and low density lipoprotein (LDL) were very significantly different between male and female Wistar rats of either conventional or SPF grade (P<0.01), which also had significant difference in albumin, lactate dehydrogenase (LDH) and apolipoprotein B (ApoB) (P<0.05).Between male aged Wistar rats of the two grades, the differences of TP, albumin, albumin/globulin (A/G) ratio, TC, TG, blood

  7. Identifying the AD 1257 Salamas volcanic event from micron-size tephra composition in two East Antarctic ice cores

    Science.gov (United States)

    Petit, Jean Robert; Narcisi, Biancamaria; Batanova, Valentina G.; Joël, Savarino; Komorowski, Jean Christophe; Michel, Agnes; Metrich, Nicole; Besson, Pascale; Vidal, Celine; Sobolev, Alexander V.

    2016-04-01

    A wealth of valuable data about the history of explosive volcanic history can be extracted from polar ice successions. Both the volatile by-products and the solid silicate (tephra) components of volcanic plumes can be incorporated into snow layers, providing tools for chronostratigraphic correlations and for interpretation of climate-volcanism interactions. Volcanic events from low-latitude regions are of particular interest as the related sulphate aerosol travelling through the stratosphere can reach the polar sheets forming inter-hemispheric (Greenland and Antarctica) signals preserved in the ice. Within the glaciological record of globally significant volcanic markers, the AD1259 signal represents one of most prominent events over the last thousands years. Its source has been long debated. On the basis of recent field investigations (Lavigne et al., 2013; Vidal et al., 2015), it has been proposed that Mount Samalas on Lombok Island (Indonesia) represents the source responsible for the polar event. With the goal of bringing distal tephrochronological evidence to source identification, we have attempted to identify volcanic ash associated to the AD 1259 sulphate pulse. To this purpose we used firn and ice-core samples from two East Antarctic Plateau sites: Concordia-Dome C (75°06' S, 123°20' E, 3233 m) and Talos Dome (72°49'S, 159°11'E, 2315 m). Our high-resolution studies included sample processing in a Class 100 clean room using established ultra-clean procedures for insoluble microparticle analyses, Coulter counter grain size measurements, scanning electron microscope observations and the geochemical (major elements) composition from the recently set ISTERRE Jeol JXA 8230 Superprobe and calibrated for small particles analysis. Despite the difficulty of studying such minute fragments, within both cores we located and characterised multiple tiny (micron-size) glass shards concomitant with the volcanic peak. We present preliminary results alongside comparison

  8. Primum Non Nocere: is shared decision-making the answer?

    Science.gov (United States)

    Santhirapala, Ramai; Moonesinghe, Ramani

    2016-01-01

    Surgical ambition is rising, with the Royal College of Surgeons reporting an increase in the number of procedures by a million over the past decade (Royal College of Surgeons. Surgery and the NHS in Numbers. Available from https://www.rcseng.ac.uk). Underpinning, this is a rapidly growing population, especially those in the over 85 age group, coupled with rising perioperative expertise; options for surgery are now present where conditions were once managed conservatively. Matching the right patient to the right procedure has never been so pertinent (Bader, Am Soc Anesthesiol 78(6), 2014). At the heart of these increasingly complex decisions, which may prove fatal or result in serious morbidity, lies the aspiration of shared decision-making (SDM) (Glance et al., N Engl J Med 370:1379-81, 2014). Shared decision-making is a patient-centred approach taking into account the beliefs, preferences and views of the patient as an expert in what is right for them, supported by clinicians who are the experts in diagnostics and valid therapeutic options (Coulter and Collins, Making shared decision-making a reality: no decision about me, without me, 2011). It has been described as the pinnacle of patient-centred care (Barry et al., N Engl J Med 366:780-1, 2012). In this commentary, we explore further the concept of shared decision-making, supported by a recent article which highlights critical deficits in current perioperative practice (Ankuda et al., Patient Educ Couns 94(3):328-33, 2014). This article was chosen for the purposes of this commentary as it is a large study across several surgical specialties investigating preoperative shared decision-making, and to our knowledge, the only of this kind. PMID:27313845

  9. Quantificação de células CD 34+ em sangue periférico, produto de aférese e cordão umbilical: estudo comparativo de três diferentes metodologias

    Directory of Open Access Journals (Sweden)

    Bacal Nydia S.

    2001-01-01

    Full Text Available A quantificação das células CD34+ em sangue periférico é utilizada para determinar o melhor momento de iniciar a aférese, enquanto que na leucoaférese e no sangue de cordão umbilical determinam a quantidade de células CD34+ para o transplante de células progenitoras. O objetivo deste trabalho foi comparar três diferentes metodologias de quantificação de células CD34+. Foram utilizados três diferentes tipos de amostras: a 32 amostras de sangue periférico, coletadas de pacientes estimulados com G-CSF 50 mg/Kg/dose total, sem quimioterapia na mobilização. b 31 amostras de produto de aférese de pacientes estimulados com o mesmo protocolo de G-CSF. c 20 amostras de sangue de cordão coletadas em CPDA-1, por punção de veia umbilical. As amostras permaneceram à temperatura ambiente no máximo até 24h da análise. O citômetro de fluxo utilizado foi o Epics XL-MCL (Coulter com os protocolos de dupla plataforma: ISHAGE e Mulhouse modificado para análise de maior numero de eventos, e o citômetro Imagn 2000 (Biometric Imaging de plataforma única, conforme técnicas recomendadas. Os anticorpos monoclonais utilizados foram: CD45-FITC, CD34-PE, e isotipo IgG1-PE da Immunotech. As análises estatísticas foram: ANOVA e correlação de teste t de Student. Os resultados não apresentaram diferenças estatisticamente significativas nos três métodos.

  10. Bio-optical observations of the North Atlantic Spring Bloom from continuous underway surface measurements

    Science.gov (United States)

    Westberry, T. K.; Dall'Olmo, G.; Behrenfeld, M. J.; Boss, E.

    2008-12-01

    The evolution of the North Atlantic Spring Bloom was observed using continuous, underway measurements of particle absorption and scattering indices in a ~10000 km2 area centered on 61N, 26W. Spectral particulate beam attenuation (cp), absorption (ap), and backscattering (bbp) were measured simultaneously using a novel flow-through system with an actuated valve and timer to intermittently direct seawater flow through 0.2 um filters. Initially, the phytoplankton community was dominated by large chain-forming diatoms, which were then succeeded by dinoflagellates and ultimately by picoeukaryotes, as revealed by flow cytometry, microscopy, and HPLC pigment analysis. Despite the wide range in taxonomic variability, consistent relationships between cp and bbp were observed. Further, the slope of this relationship was similar to that obtained in very different oceanic environments (from oligotrophic to mesotrophic). This covariability of the two scattering indices suggests that they are, in large part, sensitive to the same pool of particles or that the particle size distribution is relatively conserved across large gradients in bulk chlorophyll (and presumably chlorophyll degradation products, as well). Coulter counter-based particle size distributions ranged from perfectly Junge-like to having pronounced peaks over the size range investigated (2-60um). In contrast to previous studies in the region, the bio-optical imprint of coccolithophorids was not observed in any of the quantities measured. Empirical relationships between optical measurements (cp, ap, bbp) with coincident biogeochemical measurements such as chlorophyll, particulate organic carbon, and biovolume-estimated phytoplankton specific carbon will also be discussed.

  11. Prevalence of Anemia and Associated Factors in Child Bearing Age Women in Riyadh, Saudi Arabia

    Directory of Open Access Journals (Sweden)

    AlJohara M. AlQuaiz

    2013-01-01

    Full Text Available Objective. To determine the prevalence and risk factors for anemia in child bearing age women in Riyadh, Saudi Arabia. Design. Cross-sectional survey was conducted using two-stage cluster sampling. 25 clusters (primary health care centers (PHCC were identified from all over Riyadh, and 45–50 households were randomly selected from each cluster. Eligible women were invited to PHCC for questionnaire filling, anthropometric measurements, and complete blood count. Blood hemoglobin was measured with Coulter Cellular Analysis System using light scatter method. Setting. PHCC. Subjects. 969 (68% women out of 1429 women were included in the analysis. Results. Mean hemoglobin was 12.35 (±1.80 g/dL, 95% CI 12.24–12.46 with interquartile range of 1.9. Anemia (Hb <12 g/dL was present in 40% (390 women. Mean (±SD for MCH, MCV, MCHC, and RDW was 79.21 (±12.17 fL, 26.37 (±6.21 pg, 32.36 (±4.91 g/dL, and 14.84 (±4.65%, respectively. Multivariate logistic regression revealed that having family history of iron deficiency anemia (OR 2.91, 95% CI 1.78–4.76 and infrequent intake of meat (OR 1.54, 95%CI 1.15–2.05 were associated with increased risk of anemia, whereas increasing body mass index (OR 0.95, 95% CI 0.92–0.97 was associated with reduced risk of anemia. Conclusion. Women should be educated about proper diet and reproductive issues in order to reduce the prevalence of anemia in Saudi Arabia.

  12. Classification of biological cells using a sound wave based flow cytometer

    Science.gov (United States)

    Strohm, Eric M.; Gnyawali, Vaskar; Van De Vondervoort, Mia; Daghighi, Yasaman; Tsai, Scott S. H.; Kolios, Michael C.

    2016-03-01

    A flow cytometer that uses sound waves to determine the size of biological cells is presented. In this system, a microfluidic device made of polydimethylsiloxane (PDMS) was developed to hydrodynamically flow focus cells in a single file through a target area. Integrated into the microfluidic device was an ultrasound transducer with a 375 MHz center frequency, aligned opposite the transducer was a pulsed 532 nm laser focused into the device by a 10x objective. Each passing cell was insonfied with a high frequency ultrasound pulse, and irradiated with the laser. The resulting ultrasound and photoacoustic waves from each cell were analyzed using signal processing methods, where features in the power spectra were compared to theoretical models to calculate the cell size. Two cell lines with different size distributions were used to test the system: acute myeloid leukemia cells (AML) and melanoma cells. Over 200 cells were measured using this system. The average calculated diameter of the AML cells was 10.4 +/- 2.5 μm using ultrasound, and 11.4 +/- 2.3 μm using photoacoustics. The average diameter of the melanoma cells was 16.2 +/- 2.9 μm using ultrasound, and 18.9 +/- 3.5 μm using photoacoustics. The cell sizes calculated using ultrasound and photoacoustic methods agreed with measurements using a Coulter Counter, where the AML cells were 9.8 +/- 1.8 μm and the melanoma cells were 16.0 +/- 2.5 μm. These results demonstrate a high speed method of assessing cell size using sound waves, which is an alternative method to traditional flow cytometry techniques.

  13. Infection-stimulated Anemia Results Primarily from Interferon Gamma-dependent, Signal Transducer and Activator of Transcription 1-independent Red Cell Loss

    Institute of Scientific and Technical Information of China (English)

    Zheng Wang; Dong-Xia Zhang; Qi Zhao

    2015-01-01

    Background:Although the onset of anemia during infectious disease is commonly correlated with production of inflammatory cytokines,the mechanisms by which cytokines induce anemia are poorly defined.This study focused on the mechanism research.Methods:Different types of mice were infected perorally with Toxoplasma gondii strain ME49.At the indicated times,samples from each mouse were harvested,processed,and analyzed individually.Blood samples were analyzed using a Coulter Counter and red blood cell (RBC) survival was measured by biotinylation.Levels of tumor necrosis factor-α (TNF-α),inducible nitric oxide synthase (iNOS),and inducible protein 10 (IP-10) mRNA in liver tissue were measured by real-time polymerase chain reaction.Results:T.gondii-infected mice exhibited anemia due to a decrease in both erythropoiesis and survival time of RBC in the circulation (P < 0.02).In addition,infection-stimulated anemia was associated with fecal occult,supporting previous literature that hemorrhage is a consequence of T.gondii infection in mice.Infection-induced anemia was abolished in interferon gamma (IFNγ) and IFNγ receptor deficient mice (P < 0.05) but was still evident in mice lacking TNF-α,iNOS,phagocyte NADPH oxidase or IP-10 (P < 0.02).Neither signal transducer and activator of transcription 1 (STAT1) deficient mice nor 129S6 controls exhibited decreased erythropoiesis,but rather suffered from an anemia resulting solely from increased loss of circulating RBC.Conclusions:Infection-stimulated decrease in erythropoiesis and losses of RBC have distinct mechanistic bases.These results show that during T.gondii infection,IFNγ is responsible for an anemia that results from both a decrease in erythropoiesis and a STAT1 independent loss of circulating RBC.

  14. The Origin and Age of Scallop Floodplain Benches from Difficult Run, Fairfax County, Virginia.

    Science.gov (United States)

    Scamardo, J. E.; Pizzuto, J. E.; Skalak, K.; Benthem, A.

    2015-12-01

    Sediment is deposited within scallop-shaped erosional scarps that form between trees armoring the banks of Difficult Run, a suburban watershed with a forested riparian zone. These deposits create small (surface area 85 m2, volume 300 m3), low-lying floodplain landforms this group terms Scallop Floodplain Benches (SFB). It is hypothesized that SFB formed within the past couple decades initially forming as transversal accretion deposits and eventually gaining floodplain features dominated by vertical accretion. Stratigraphic data supports that SFB deposits begin laterally as sand and gravel bars approximately 100 cm thick, and continue to grow by vertical accretion of sand, silt, and clay. As a SFB reaches its maximum height, a distinctive levee develops adjacent to the channel, and fine-grained silt and clay are deposited behind the levee. Core samples to a depth of 118 cm and additional samples from an overbank event that occurred on June 20, 2015 were collected from one of two SFB on Difficult Run near Leesburg Pike. The grain size distribution was measured using a Coulter Counter and activities of Pb-210, Cs-137, and Be-7 were measured using High Purity Germanium Detectors. Cs-137 activities are relatively constant with depth without a well-defined peak, suggesting that the SFB was deposited after 1963. Be-7 is present in the recent flood deposits, but is absent below the surface, suggesting that the SFB deposits are at least several years old. Excess Pb-210 activities decrease exponentially with depth, and can be fit using the Constant Rate of Supply method to determine an average age of approximately 13.5 years for the SFB. The SFB is storing sediment at a rate of 27 tons/year, which is equal to 0.35% of the annual sediment load of Difficult Run, based on this average age. SFB appear to be a significant component of the sediment storage of Difficult Run and therefore should be considered in the sediment budget.

  15. Feasibility of using a particle counter or flow-cytometer for bacterial enumeration in the assimilable organic carbon (AOC) analysis method.

    Science.gov (United States)

    Aggarwal, Srijan; Jeon, Youchul; Hozalski, Raymond M

    2015-09-01

    Assimilable organic carbon (AOC) is one of the major determinants of microbial growth and stability in drinking water distribution systems. Nevertheless, AOC measurements are rarely conducted in practice owing, in part, to the tedious and time-consuming nature of the bioassay. Herein, we compared three alternative cell count approaches [flow cytometry with staining (FC-S), flow cytometry without staining (FC-NS), and particle counting (Coulter counter; CC)] for bacterial enumeration as a means to expedite the AOC bioassay. Our results suggest that of the three methods only FC-S provides a suitable alternative to plate counting for rapid and accurate enumeration of both P17 and NOX in the AOC bioassay. While the cell counts obtained by FC-NS were linearly correlated with those obtained using the traditional heterotrophic plate count (HPC) method (FC-NS: R(2) = 0.89-0.96), the AOC values obtained by FC-NS were overestimated by 18-57 %. The CC approach was unsuccessful in enumerating Spirillum strain NOX cells because of the relatively small size of that organism. The CC counts were linearly correlated with HPC for Pseudomonas fluorescens strain P-17 (P17) cells (R(2) = 0.83) but like FC-NS, the CC approach also overestimated the AOC values (for P-17). The advantage of the FC-S method over the other two is improved sensitivity and the ability to specifically enumerate whole cells (and likely viable) as opposed to non-viable cells, cell debris, and other contaminating particles introduced by the test water itself or sample handling. PMID:26139595

  16. Formation of microscopic particles during the degradation of different polymers.

    Science.gov (United States)

    Lambert, Scott; Wagner, Martin

    2016-10-01

    This study investigated the formation and size distribution of microscopic plastic particles during the degradation of different plastic materials. Particle number concentrations in the size range 30 nm-60 μm were measured by nanoparticle tracking analysis (NTA) and Coulter Counter techniques. Each of the plastics used exhibited a measureable increase in the release of particles into the surrounding solution, with polystyrene (PS) and polylactic acid (PLA) generating the highest particle concentrations. After 112 d, particle concentrations ranged from 2147 particles ml(-1) in the control (C) to 92,465 particles ml(-1) for PS in the 2-60 μm size class; 1.2 × 10(5) particles ml(-1) (C) to 11.6 × 10(6) for PLA in the 0.6-18 μm size class; and 0.2 × 10(8) particles ml(-1) (C) to 6.4 × 10(8) particles ml(-1) for PS in the 30-2000 nm size class (84 d). A classification of samples based on principal component analysis showed a separation between the different plastic types, with PLA clustering individually in each of the three size classes. In addition, particle size distribution models were used to examine more closely the size distribution data generated by NTA. Overall, the results indicate that at the beginning of plastic weathering processes chain scission at the polymer surface causes many very small particles to be released into the surrounding solution and those concentrations may vary between plastic types. PMID:27470943

  17. Macronutrients leaf contents of corn in intercropping with forages of genus Panicum and Urochloa in simultaneous seeding

    Directory of Open Access Journals (Sweden)

    Denise Tsuzukibashi

    2013-12-01

    Full Text Available The planting of crops in degraded pasture areas is a formula used for decades by farmers to recover the productive capacity of pastures and soils. The integrated crop-livestock (ICL consists of different production systems of grains, fibers, wood, meat, milk and agro-deployed in the same area, in intercrop, rotation or succession. Typically this integration mainly involves the planting of grain and pasture in the recovery or deployment. This work aimed to evaluate the macronutrients leaf contents of irrigated corn intercropped with forages of the genus Panicum and Urochloa simultaneously to sown corn. The experiment was conducted at the Farm for Teaching, Research and Extension, Faculty of Engineering - UNESP, Ilha Solteira in an Oxisol in Savannah conditions, in experimental area that had a history of no-tillage to 8 years (previous crop corn. The experimental design used was randomized blocks with four replications and five treatments: Panicum maximum cv. Tanzania sown simultaneously (CTS corn; Panicum maximum cv. Mombaça sown simultaneously (CMS to corn; Urochloa brizantha cv. Xaraes sown simultaneously (CBS corn; Urochloa ruziziensis sown simultaneously (CRS to corn, and corn without intercropping (CWI. The seeds of grasses were sown in spacing of 0.34 m, being sown with a seed drill with disc coulters mounted mechanism for no-tillage system at a depth of 0.03 m. There was no significant difference between the single corn tillage and intercropping with different modalities of forage genus Panicum and Urochloa to the leaf contents of N, P, K, Ca and Mg, demonstrating the non-compete forages with corn in a intercrop on the absorption of these nutrients. In respect to S, CTS presented higher content of S foliar when compared to CWI (Table 1. The absorption of nutrients by corn are not affect by the intercrop with forages of the genus Panicum and Urochloa, in simultaneously sown.

  18. Prevalence of Beta-Thalassemia in premarital screening in Al-Hassa, Saudi Arabia

    International Nuclear Information System (INIS)

    The Al-Hassa area is one of the regions in Saudi Arabia where hemoglobinopathies are prevalent. The Saudi Ministry of Health designed a protocol for premarital testing after the royal decree in December 2003. The protocol was implemented in a February 2004 order. The aim of this study was to determine the prevalence of beta-thalassemia trait among subjects coming for premarital screening in the Al-Hassa area. From February 2004 to November 2004, healthy subjects coming to six marriages consultation centers in the Al-Hassa area underwent routine mandatory tests. Subjects were considered to have beta-thalassemia trait if they had a mean corpuscular volume (MCV), 80 fL and/or a mean corpuscular hemoglobin (MCH) 3.2%. Venous blood was taken into ETDA tube and the complete blood count and red blood cell indices were measured by a Coulter automated cell counter on the same day of hemoglobin collection. Electrophoresis was done on cellulose acetate. All Saudi participants (n=8918), including 4218 (47.3%) males and 4700 (52.7%) females were screened. The prevalence of beta-thalassemia trait with high hemoglobin A2 and microcytic hypochromic anemia was 3.4% (307/8918). In countries with a high prelevance of hemoglobinopathies, a premarital screening program is helpful for identification and prevention of high-risk marriages. With a 3.4% prevalence of beta-thalassemia trait in premarital couples, future comprehensive programs are needed to know the actual prevalence of beta-thalassemia in Al-Hassa. (author)

  19. Cell diameter measurements obtained with a handheld cell counter could be used as a surrogate marker of G2/M arrest and apoptosis in colon cancer cell lines exposed to SN-38

    Energy Technology Data Exchange (ETDEWEB)

    Tahara, Makiko [Oncogene Research Unit/Cancer Prevention Unit, Tochigi Cancer Center Research Institute, Utsunomiya, Tochigi (Japan); Department of Gastrointestinal Surgery, Jichi Medical University, Shimotsuke, Tochigi (Japan); Inoue, Takeshi [Oncogene Research Unit/Cancer Prevention Unit, Tochigi Cancer Center Research Institute, Utsunomiya, Tochigi (Japan); Miyakura, Yasuyuki; Horie, Hisanaga; Yasuda, Yoshikazu [Department of Gastrointestinal Surgery, Jichi Medical University, Shimotsuke, Tochigi (Japan); Fujii, Hirofumi [Division of Clinical Oncology, Jichi Medical University, Shimotsuke, Tochigi (Japan); Kotake, Kenjiro [Department of Surgery, Tochigi Cancer Center, Utsunomiya, Tochigi (Japan); Sugano, Kokichi, E-mail: ksugano@tcc.pref.tochigi.lg.jp [Oncogene Research Unit/Cancer Prevention Unit, Tochigi Cancer Center Research Institute, Utsunomiya, Tochigi (Japan)

    2013-05-17

    Highlights: •Chemo-sensitivity to SN-38 was assayed by the automated cell counter. •Colon cancer cell line, HCT116 cells were more sensitive to SN-38 than HT29 cells. •Increase of cell size reflects G2/M arrest. •Appearance of small particles indicates cell apoptosis. -- Abstract: In vitro assessment of chemosensitivity are important for experiments evaluating cancer therapies. The Scepter 2.0 cell counter, an automated handheld device based on the Coulter principle of impedance-based particle detection, enables the accurate discrimination of cell populations according to cell size and volume. In this study, the effects of SN-38, the active metabolite of irinotecan, on the colon cancer cell lines HCT116 and HT29 were evaluated using this device. The cell count data obtained with the Scepter counter were compared with those obtained with the {sup 3}H-thymidine uptake assay, which has been used to measure cell proliferation in many previous studies. In addition, we examined whether the changes in the size distributions of these cells reflected alterations in the frequency of cell cycle arrest and/or apoptosis induced by SN-38 treatment. In our experiments using the Scepter 2.0 cell counter, the cell counts were demonstrated to be accurate and reproducible measure and alterations of cell diameter reflected G2/M cell cycle arrest and apoptosis. Our data show that easy-to-use cell counting tools can be utilized to evaluate the cell-killing effects of novel treatments on cancer cells in vitro.

  20. Impedance microflow cytometry for viability studies of microorganisms

    Science.gov (United States)

    Di Berardino, Marco; Hebeisen, Monika; Hessler, Thomas; Ziswiler, Adrian; Largiadèr, Stephanie; Schade, Grit

    2011-02-01

    Impedance-based Coulter counters and its derivatives are widely used cell analysis tools in many laboratories and use normally DC or low frequency AC to perform these electrical analyses. The emergence of micro-fabrication technologies in the last decade, however, provides a new means of measuring electrical properties of cells. Microfluidic approaches combined with impedance spectroscopy measurements in the radio frequency (RF) range increase sensitivity and information content and thus push single cell analyses beyond simple cell counting and sizing applications towards multiparametric cell characterization. Promising results have been shown already in the fields of cell differentiation and blood analysis. Here we emphasize the potential of this technology by presenting new data obtained from viability studies on microorganisms. Impedance measurements of several yeast and bacteria strains performed at frequencies around 10 MHz enable an easy discrimination between dead and viable cells. Moreover, cytotoxic effects of antibiotics and other reagents, as well as cell starvation can also be monitored easily. Control analyses performed with conventional flow cytometers using various fluorescent dyes (propidium iodide, oxonol) indicate a good correlation and further highlight the capability of this device. The label-free approach makes on the one hand the use of usually expensive fluorochromes obsolete, on the other hand practically eliminates laborious sample preparation procedures. Until now, online cell monitoring was limited to the determination of viable biomass, which provides rather poor information of a cell culture. Impedance microflow cytometry, besides other aspects, proposes a simple solution to these limitations and might become an important tool for bioprocess monitoring applications in the biotech industry.

  1. On the relationship between band broadening and the particle-size distribution of the packing material in liquid chromatography: theory and practice.

    Science.gov (United States)

    Gritti, Fabrice; Farkas, Tivadar; Heng, Josuah; Guiochon, Georges

    2011-11-11

    The influence of the particle size distribution (PSD) on the band broadening and the efficiency of packed columns is investigated on both theoretical and practical viewpoints. Each of the classical contributions to mass transfer kinetics, those due to longitudinal diffusion, eddy dispersion, and solid-liquid mass transfer resistance are measured and analyzed in terms of their expected and observed intensity as a function of the PSD of mixtures of the commercially available packing materials, 5 and 3 μm Luna-C₁₈ particles (Phenomenex, Torrance, CA, USA). Six 4.6 mm × 150 mm columns were packed with different mixtures of these two materials. The efficiencies of these columns were measured for a non-retained and a retained analytes in a mixture of acetonitrile and water. The longitudinal diffusion coefficient was directly measured by the peak parking method. The solid-liquid mass transfer coefficient was measured from the combination of the peak parking method, the best model of effective diffusion coefficient and the actual PSDs of the different particle mixtures measured by Coulter counter experiments. The eddy diffusion term was measured according to a recently developed protocol, by numerical integration of the peak profiles. Our results clearly show that the PSD has no measurable impact on any of the coefficients of the van Deemter equation. On the contrary and surprisingly, adding a small fraction of large particles to a batch of small particles can improve the quality of the packing of the fine particles. Our results indirectly confirm that the success of sub-3 μm shell particles is due to the roughness of their external surface, which contributes to eliminate most of the nefarious wall effects.

  2. Correlation of Serum Concentrations of Soluble Thrombomodulin, Soluble Vascular Cell Adhesion Molecule-1,Intracellular Adhesion Molecule -1 And E-Selectin In Patients WithSystemic Lupus Erythematosus

    Directory of Open Access Journals (Sweden)

    Malak., A. Mohsen*, Magda.A.Gamil*,Maha. I.Shehata

    2003-09-01

    Full Text Available To date no specific serological parameters are available to assess disease activity in systemic lupus erythematosus (SLE. The objective of this study was to correlate serum levels of thrombomodulin (TM, intracellular adhesion molecule-1 sICAM-1, vascular cell adhesion molecule-1 sVCAM-1, and E-selectin with standard laboratory tests and clinical indices of disease activity in 40 patients with SLE and 20 apparently healthy persons as controls. According to British Isles Lupus Assessment Group (BILAG disease activity index, the 40 patients were divided into two groups, the first consisted of 22 with active disease, and the second consisted of 18 patients with inactive SLE. Serum sTM, sICAM-1, sVCAM-I, and E-selectin were measured in their sera, using enzyme linked immuonosorbent assay (ELISA technique.C-reactiv protein (CRP, Erythrocyte sedimentation rates (ESR and serum creatinines were measured by standard laboratory tests. Total leukocytic count and hemoglobin concentration were detected by coulter counter. Levels of sTM and sVCAM were highly elevated in the group of patients with active SLE as compared to the inactive one (P0.05. In SLE, the BILAG disease activity index, ESR and serum creatinine correlated best with sTM, sVCAM-1 and E-selectin levels while there was a weak association between CRP levels and the adhesion molecules, and no correlation between CRP level and disease activity. In conclusion, sTM and sVCAM were the most important serological indices of disease activity in SLE and might be valuable serological parameters for monitoring therapy.

  3. THE EFFECTS OF SUBMAXIMAL EXERCISES ON THE THYROID HORMONAL METABOLISM OF SEDENTARY INDIVIDUALS

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    Mustafa AKIL

    2011-04-01

    Full Text Available The objective of this study is to analyze the effects of acute exercises the sedentary individuals do on thyroid hormonal metabolism. The research was carried out on 14 sedentary males between the ages of 18 and 24. The study was approved by the local committee for ethics. The average age of the subjects was: 20,4 ± 0,65 years, their average body weights: 76,86 ± 2,08 kg and their average height was: 175,07 ± 1,26 cm. As for the exercise application, the subjects were made tired through Bruce protocol.10 cc of blood samples were taken from the subjects each time before the exercise, after the exercise, 24 hours after the exercise and 48 hours after the exercise. In the obtained blood samples, the parameters of serum TSH, T3, T4, were analyzed in Backman LX-20 auto analyzer through Lot No:T1142, Mg Lot No:907035, P Lot No:903417 Backman Coulter test kits. The arithmetic averages, and standard deviations of all the parameters were determined. The repeating ANOVA test was used for the determination of the difference between the parameters. Confidence interval was chosen as 95% and the values of p≤0.05 and below was accepted as statistically significant. According to the measurements before and after the exercises, significant differences were found between the parameters of TSH, T3, and T4 (p≤0.05.In conclusion, it can be said that the protocol of these acute submaximal exercises done by sedentary individuals decreased serum TSH, T3, and T4 levels and it approached to normal levels 48 hours after the exercises.

  4. Membrane permeability of the human granulocyte to water, dimethyl sulfoxide, glycerol, propylene glycol and ethylene glycol.

    Science.gov (United States)

    Vian, Alex M; Higgins, Adam Z

    2014-02-01

    Granulocytes are currently transfused as soon as possible after collection because they rapidly deteriorate after being removed from the body. This short shelf life complicates the logistics of granulocyte collection, banking, and safety testing. Cryopreservation has the potential to significantly increase shelf life; however, cryopreservation of granulocytes has proven to be difficult. In this study, we investigate the membrane permeability properties of human granulocytes, with the ultimate goal of using membrane transport modeling to facilitate development of improved cryopreservation methods. We first measured the equilibrium volume of human granulocytes in a range of hypo- and hypertonic solutions and fit the resulting data using a Boyle-van't Hoff model. This yielded an isotonic cell volume of 378 μm(3) and an osmotically inactive volume of 165 μm(3). To determine the permeability of the granulocyte membrane to water and cryoprotectant (CPA), cells were injected into well-mixed CPA solution while collecting volume measurements using a Coulter Counter. These experiments were performed at temperatures ranging from 4 to 37°C for exposure to dimethyl sulfoxide, glycerol, ethylene glycol, and propylene glycol. The best-fit water permeability was similar in the presence of all of the CPAs, with an average value at 21°C of 0.18 μmatm(-1)min(-1). The activation energy for water transport ranged from 41 to 61 kJ/mol. The CPA permeability at 21°C was 6.4, 1.0, 8.4, and 4.0 μm/min for dimethyl sulfoxide, glycerol, ethylene glycol, and propylene glycol, respectively, and the activation energy for CPA transport ranged between 59 and 68 kJ/mol. PMID:24269528

  5. Anemia em escolares da primeira série do ensino fundamental da rede pública de Maceió, Alagoas, Brasil

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    Célia Dias dos Santos

    2002-12-01

    Full Text Available Realizou-se um estudo transversal com amostra probabilística de 426 alunos de 6 a 10 anos, da 1ª série do ensino fundamental das escolas públicas de Maceió, Alagoas, Brasil. O objetivo foi avaliar a prevalência da anemia e sua associação com o retardo de crescimento. Os dados foram coletados entre maio e julho de 2000. O sangue foi colhido por venipuntura e a dosagem de hemoglobina (Hb foi realizada pelo contador Coulter STKS. Classificou-se a anemia por dois critérios estabelecidos pela Organização Mundial da Saúde: Hb <11,5g/dl e Hb <12,0g/dl. O retardo pondo-estatural foi diagnosticado quando os indicadores Altura/Idade (A/I, Peso/Idade (P/I e Peso/Altura (P/A se encontravam abaixo de -2,0 desvios-padrão da referência NCHS. A prevalência da anemia foi de 9,9%, considerando-se Hb <11,5g/dl e de 25,4%, utilizando-se Hb <12,0g/dl. O retardo do crescimento foi detectado em 6,2% segundo A/I, 4,0% pelo indicador P/I e 3,0% em relação a P/A. Não houve associação estatisticamente significativa entre as variáveis do estudo. Estes resultados confirmam os observados em outras pesquisas: prevalência de anemia muito superior à prevalência do retardo pondo-estatural. Conclui-se que as graves conseqüências da anemia nesta faixa etária, justificam a implementação de políticas de largo alcance para o enfrentamento do problema.

  6. Comparison of cell counting methods in rodent pulmonary toxicity studies: automated and manual protocols and considerations for experimental design.

    Science.gov (United States)

    Zeidler-Erdely, Patti C; Antonini, James M; Meighan, Terence G; Young, Shih-Houng; Eye, Tracy J; Hammer, Mary Ann; Erdely, Aaron

    2016-08-01

    Pulmonary toxicity studies often use bronchoalveolar lavage (BAL) to investigate potential adverse lung responses to a particulate exposure. The BAL cellular fraction is counted, using automated (i.e. Coulter Counter®), flow cytometry or manual (i.e. hemocytometer) methods, to determine inflammatory cell influx. The goal of the study was to compare the different counting methods to determine which is optimal for examining BAL cell influx after exposure by inhalation or intratracheal instillation (ITI) to different particles with varying inherent pulmonary toxicities in both rat and mouse models. General findings indicate that total BAL cell counts using the automated and manual methods tended to agree after inhalation or ITI exposure to particle samples that are relatively nontoxic or at later time points after exposure to a pneumotoxic particle when the response resolves. However, when the initial lung inflammation and cytotoxicity was high after exposure to a pneumotoxic particle, significant differences were observed when comparing cell counts from the automated, flow cytometry and manual methods. When using total BAL cell count for differential calculations from the automated method, depending on the cell diameter size range cutoff, the data suggest that the number of lung polymorphonuclear leukocytes (PMN) varies. Importantly, the automated counts, regardless of the size cutoff, still indicated a greater number of total lung PMN when compared with the manual method, which agreed more closely with flow cytometry. The results suggest that either the manual method or flow cytometry would be better suited for BAL studies where cytotoxicity is an unknown variable. PMID:27251196

  7. Spatial and temporal variability in aggregated grain-size distributions, with implications for sediment dynamics

    Science.gov (United States)

    Wheatcroft, Robert A.; Butman, Cheryl Ann

    1997-04-01

    The grain-size distribution of bottom sediments has important implications for diverse aspects of sediment dynamics, including prediction of the critical boundary shear stress and calibration of suspended sediment sensors. Past sampling strategies to obtain estimates of the seabed grain-size distribution typically have not considered spatial and temporal variability, and have been insufficient to resolve potential millimeter-scale vertical variations in grain size. Moreover, laboratory analyses have been predicated on chemically and/or ultrasonically disaggregating the sediments before resolving particle diameter, therefore the more dynamically relevant in situ grain-size spectrum is not measured. To test for such effects, three sites on the northern California continental shelf comprising a cross-shelf transect from a sandy, inner-shelf (60 m) site, to a muddy, mid-shelf (90 m) site and a relict, outer-shelf (130 m) site were studied. Replicate box cores were collected over two winter field seasons, and multiple subcores from each box core were vertically sectioned at 2-mm intervals. Gentle wet sieving techniques were used to determine the mass fraction in the 300,um size classes. In addition, a lesser number of standard disaggregated grain-size analyses were performed using a Coulter Counter. Results from the sandy, inner-shelf site indicate the presence of an ephemeral fine-grained (flow. In addition, there is evidence for a progressive and substantial winnowing of fine-grained sediment from the surface layer over the course of a winter storm season. At the deeper sites, the upper 2 mm of the bed contained 5-20% more material meters) scale spatial variability is modest. In addition, the disaggregated grain-size distribution at the two muddy sites is, in all cases, markedly finer than the in situ grain-size distribution. Therefore, calibrations and predictions based on a knowledge of the size distribution of the primary (i.e. disaggregated) particles could be in

  8. Lymphocytes subsets in children with febrile convulsions.

    Science.gov (United States)

    Tuncer, Oğuz; Karaman, Sait; Caksen, Hüseyin; Oner, Ahmet Faik; Odabas, Dursun; Yilmaz, Cahide; Atas, Bülent

    2007-07-01

    In this study, lymphocytes subsets including blood CD3, CD4, CD8, CD16, CD19, and CD56 values were analyzed in children with febrile convulsion (FC) to determine whether there was the association of lymphocytes subsets in the pathogenesis of FC. The study includes 48 children with FC, and 55 healthy age matched control subjects, followed in Yüzüncü Yil University, Faculty of Medicine, Department of Pediatrics between October 2003 and June 2004. Blood CD3, CD4, CD8, CD16, CD19, and CD56 values were examined in the study and control groups. The analyses were performed in the Hematology Laboratory, Yüzüncü Yil University Faculty of Medicine, with flow cytometer device (Coulter Epics XL2, Flow Cytometer). A total of 48 children [17 girls (35.5%) and 31 boys (64.5%)], aged 6 months to 60 months (mean 22.20 +/- 13.75 months) with FC and 55 healthy children [28 girls (51%) and 27 boys (49%)], aged 6 months to 60 months (mean 28.87 +/- 17.04 months) were included in the study. When compared with the control group, the study found significantly decreased blood CD3 and CD4 values in the study group (p .05). When comparing the children with and without positive family history for FC, the study did not find any difference for all CD values between the groups (p >.05). Similarly, there was not significant difference in CD values between the children with simple and complex FC (p >.05). The findings suggested that decreased blood CD3 and CD4 values might be responsible for the infections connected with FC or that they might be related to the pathogenesis of FC in some children.

  9. Generation of Microcellular Biodegradable Polycaprolactone Foams in Supercritical Carbon Dioxide

    Institute of Scientific and Technical Information of China (English)

    Xu Qun; Ren Xian-wen; Chang Yu-ning; Yu Long; Wang Jing-wu

    2004-01-01

    Present now the application of microcellular polymeric materials in biomedical field is growing rapidly, as that of guided tissue regeneration and cell transplantation. As far as guided tissue regeneration is concerned, porous implants are used as size selective membrane to promote the growth of a special tissue in a healing site. Ideally, the implant should be inherently biocompatible,have well-defined cell size and be resorbable with appropriate biodegradation rates.Poly(a-caprolactone) (PCL) is a kind of materials suit for the demands above. PCL is biocompatible and biodegradable aliphatic polyester which is nontoxic for living organisms and bioresorbable after a period of implantation. Because of its unique combination of biocompatibility, permeability and biodegradability, PCL and some of its copolymer with lactides and glycolide have been widely applied in medicine as artificial skin, artificial bone and containers for sustained drug release.Goel and Beckman have reported a new method to generate microcellular poly(methy l methacrylate) foams in which the samples are saturated with CO2 under a series of supercritical (SC)conditions, and then the system is rapidly depressurized to atmospheric pressure at constant temperature. Unlike traditional methods, it reduces glass-transition temperature (Tg) of the mixture to below the experimental temperature rather than directly heat the system above Tg. In this process of nucleation, no phase separation occurs as well as no phase boundary meets, so the cellular structure of the foam can be retained better.In this work, we have generated PCL foams by using supercritical CO2. Because of the low glass transition temperature (Tg = -60 ℃) of PCL far below the ice point, the experimental temperature in our study is much higher than Tg, which is different from the studies by others before. A series of variable factors on the foam structure as saturation temperature, saturation pressure, saturation time and depressurization

  10. 蓝色鳞(Cyprinus carpio blue vat)的细胞遗传学分析%Cytogenetic Analysis of Blue Common Carp Cyprinus carpio blue var

    Institute of Scientific and Technical Information of China (English)

    薛淑群; 徐伟

    2011-01-01

    The diploid chromosome number, karyotype, and DNA content were studied in metaphase chromosome in kidney cells and blood of blue common carp Cyprinus carpio blue var injected with PHA and colchicin by airdrying technique. It was found that the diploid chromosome number was 2n=100. The karyotype was found to be composed of 30 m, 26sm and 44st+t chromosomes, and NF=156. The diploid nucleus DNA content was measured from the somatic cell of the blue common carp using flow cytometer (Beckman cellLab Quant, made in USA) and the DNA content (2.3 pg· N^+) in erythrocytes of chick (Gallus sp) as standard. The common carp had DNA content of3.99pg·N^-1, corresponding to the result measured chromosome number as ploidy.%采用体内注射PHA和秋水仙素,肾细胞短期培养,常规空气干燥法制备蓝色鳞鲤染色体,对100个中期分裂相记数统计,确定蓝色鳞鲤(Cyprinus carpio blue var)的染色体数为2n=100。测得核型参数按Levan等的染色体划分标准得出:蓝色鳞鲤有15对中部着丝点染色体(m);13对亚中部着丝点染色体(sin);22对端部和亚端部着丝点染色体(st,t),其染色体总臂数(NF)为156,蓝色鳞鲤核型公式为2n=30m+26sm+44st,t。采用流式细胞分析仪测定了蓝色鳞鲤的DNA含量,与鸡血细

  11. 58例妊娠高血压疾病凝血四项结果分析%The Study on Four Blood Coagulation Indexes of 58 Cases of Gestational Hypertension

    Institute of Scientific and Technical Information of China (English)

    刘淑玲; 武雪征; 任淑媛

    2011-01-01

    Objective To detect the function of blood coagulation for pregnant women with gestational hypertension, and to elucidate its clinical significance and application. Methods The results of prothrombin time,activated partial thromboplastin time, fibrinogen( FIB ), D-Dimer ( D-D )for pregnant women with or without hypertension( each group 58 cases ), and randomly selected normal unpregnant women( 88 cases ) were compared using BECKMAN( ACL9000 )to test the four parameters of blood coagulation. Results Blood coagulation tests showed that FIB and D-D of pregnant women were slightly or notably increased compared with unpregnant women( P < 0.01 ). FIB and D-D of pregnant women with hypertension were higher than those of normal pregnancy( P < 0.01 ). Conclusion Regular inspection of the blood coagulation for pregnant women is significant to early diagnosis and treatment for the gestational hypertension, which is also a guidance to monitor the pregnant women' s blood coagulation, providing scientfic laboratory basis for the pregnant woman health and normal fetus development.%目的通过对妊娠高血压疾病的妇女凝血四项结果的分析,总结其变化规律并应用于临床.方法用美国贝克曼ACL9000血凝仪对正常妊娠和患有妊娠期高血压疾病患者(各58例)及随机抽取的正常非妊娠妇女(88例为对照组)进行血凝四项的测定:凝血酶原时间、活化部分凝血酶时间、纤维蛋白原(FIB)、D-二聚体(D-D),并进行分析.结果妊娠高血压组血浆FIB、D-D的含量均高于非妊娠组(P<0.01).妊娠高血压疾病组的FIB及D-D高于正常妊娠组(P<0.01).结论定期检测孕妇血浆中凝血四项,对妊娠高血压疾病的早期诊断和治疗具有重要意义,対妊娠孕妇凝血机制的监测具有指导意义,为孕妇健康和胎儿的正常发育提供科学的实验依据.

  12. Capillary electrophoresis for the assay of fixed-dose combination tablets of artesunate and amodiaquine

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    Amin N’Cho

    2012-05-01

    Full Text Available Abstract Background Quality control of drugs in formulations is still a major challenge in developing countries. For the quality control of artesunate and amodiaquine tablets in fixed-dose combination, only liquid chromatographic methods have been proposed in the literature. There are no capillary electrophoretic methods reported for the determination of these active substances, although this technique presents several advantages over liquid chromatography (long lifetime, low price of the capillary, low volumes of electrolyte consumption in addition to simplicity. In this paper, a reliable capillary electrophoresis method has been developed and validated for the quality control of these drugs in commercial fixed-dose combination tablets. Methods Artesunate and amodiaquine hydrochloride in bilayer tablets were determined by micellar electrokinetic capillary chromatography (MEKC. Analytes were extracted from tablets by sonication with a solvent mixture phosphate buffer pH 7.0-acetonitrile containing benzoic acid as internal standard. Separation was carried out on Beckman capillary electrophoresis system equipped with fused silica capillary, 30 cm long (20 cm to detector × 50 μm internal diameter, using a 25 mM borate buffer pH 9.2 containing 30 mM sodium dodecyl sulfate as background electrolyte, a 500 V cm−1 electric field and a detection wavelength of 214 nm. Results Artesunate, amodiaquine and benzoic acid were separated in 6 min. The method was found to be reliable with respect to specificity,linearity of the calibration line (r2 > 0.995, recovery from synthetic tablets (in the range 98–102%, repeatability (RSD 2–3%, n = 7 analytical procedures. Application to four batches of commercial formulations with different dosages gave content in good agreement with the declared content. Conclusion The MEKC method proposed is reliable for the determination of artesunate and amodiaquine hydrochloride in fixed

  13. Evaluation of the Serum Zinc Level in Erosive and Non-Erosive Oral Lichen Planus

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    Gholizadeh N.

    2013-10-01

    Full Text Available Statement of Problem: Lichen planus is a chronic inflammatory immunologic-based disease involving skin and mucosa. This disease is generally divided into two categories: erosive and non-erosive. Many etiologic factors are deliberated regarding the disease; however, the disorders of immune system and the role of cytotoxic T-lymphocytes and monocytes are more highlighted. Zinc is an imperative element for the growth of epithelium and its deficiency induces the cytotoxic activity of T-helper2 cells which seems to be associated with lichen planus. Purpose: This study aimed to evaluate the levels of serum zinc in erosive and non-erosive oral lichen planus (OLP and compares it with the healthy control group to find out any feasible inference. Materials and Method: A total of 22 patients with erosive oral lichen planus, 22 pa-tients with non erosive OLP and 44 healthy individuals as the control group were recruited in this descriptive-comparative study. All the participants were selected from the referees to the department of oral medicine, school of dentistry, Tabriz University of medical sciences. Serum zinc level was examined for all the individuals with liquid-stat kit (Beckman Instruments Inc., Carlsbad, CA. Data were analyzed by adopting the ANOVA and Tukey tests through SPSS 16 statistical software. Results: The mean age of patients with erosive and non-erosive LP was 41.7 and 41.3 years, respectively. The mean age of the healthy control group was 34.4 years .The mean serum zinc levels in the erosive and non erosive lichen planus groups and control groups were 8.3 (1.15, 11.15 (0.92 and 15.74 (1.75 μg/dl respectively. The difference was statistically significant (p< 0.05. Conclusion: The serum zinc levels were decreased in patients with erosive oral lichen planus. This finding may probably indicate the promising role of zinc in development of oral lichen planus.

  14. White blood cells of peripheral blood with ConA-positive glycotopes in patients with chronic leukemia

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    G. S. Maslak

    2015-09-01

     Tumor growth progression of blood cells occurs due to changes in their genetic apparatus, which affects not only the cells morphological characteristics, but also their functional activity which to a greater extent depends on the membrane surface structures, a significant part of which is of glycoprotein nature. Complex type N-glycans are components of surface glycoproteins in the most of leukocytes. Thus, the study of changes in carbohydrate determinants of glycoproteins on the surface of leucocytes in tumorigenesis can help to reveal the mechanisms of this process. The aim of our study was to investigate the monocytes and granulocytes cytoplasmic membrane N-glycosylation in patients with chronic leukemia. The object of the study were blood cells of patients with chronic lymphocytic leukemia (n = 12 and polycythemia vera (n = 15 aged 58–66 years. Healthy hematologic volunteers (n = 15 aged 55 to 65 years were in the control group. N-glycan exposure on monocytes and granulocytes was investigated by flow cytometer Beckman Сoulter EPICS with Canavalia ensiformis lectin – Con A conjugated with fluorescent labels. The number of dead cells was monitored by means of binding them with propidium iodide. The result has been analyzed with FC Express. According to our data, levels of ConA-positive monocytes and granulocytes were 9,9 ± 1,0% and 32,7 ± 3,2%, respectively, in peripheral blood of healthy persons. The level of ConA-positive monocytes decreased to 31,0 ± 2,3% and the number of ConA-binding granulocytes increased to 66,7 ± 3,8% in patients with chronic lymphocytic leukemia compared with the norm. The number of ConA-positive monocytes decreased 3.3 times, and the level of granulocytes interacting with Canavalia ensiformis lectin slightly increased relative to control in polycythemia vera patients. There is significant increase in Con A-positive epitopes on granulocytes in patients with chronic lymphocytic leukemia and polycythemia vera compared with the

  15. Analysis on early diagnosis of hypertensive and type 2 diabetes nephropathies with cystatin C and retinol binding protein%血清半胱氨酸蛋白酶抑制剂C、视黄醇结合蛋白联合检测在诊断早期肾损伤中的临床意义

    Institute of Scientific and Technical Information of China (English)

    邵耀明; 倪芳颖; 马坚; 王琼

    2012-01-01

    目的 探讨血清半胱氨酸蛋白酶抑制剂C(Cystatin C,Cys C)和视黄醇结合蛋白(retinol binding protein,RBP)联合检测对诊断原发性高血压、2型糖尿病患者早期肾损害的临床价值.方法 采用Beckman-Coulter全自动生化仪分别检测185例原发性高血压患者、193例2型糖尿病患者及30例健康对照组血清半胱氨酸蛋白酶抑制剂C、视黄醇结合蛋白、尿素氮(BUN)、肌酐(SCr)的含量.结果 高血压及2型糖尿病患者血清半胱氨酸蛋白酶抑制剂C、视黄醇结合蛋白水平均显著高于对照组(P 0.05).结论 血清半胱氨酸蛋白酶抑制剂C、视黄醇结合蛋白可作为诊断患者早期肾损害的敏感指标.%Objective To explore the clinical significance of determining cystatin C (Cys C) and retinol binding protein (RBP) in diagnosis of early renal damage in patients with hypertension and type 2 diabetes. Methods Cys C and RBP were detected in 185 hypertension patients and 193 type 2 diabetes patients and 30 controls with auto chemistry analyzer, BUN and SCr were detected at the same time. Results Cys C and RBP in patients with hypertension and type 2 diabetes group were increased significantly compared with those of the control group (P 0.05). Conclusion Cys C and RBP are good diagnostic markers of early renal damage in patients with hypertension and type 2 diabetes. The combined examination of Cys C and RBP can be more helpful for the diagnosis of early renal damage.

  16. 胃癌组织中游离氨基酸变化与肿瘤进展程度的关系%Changes of free amino acids in gastric cancer tissue and it's relationship with cancer stages

    Institute of Scientific and Technical Information of China (English)

    梁晓宇; 王鹏志; 朱理玮; 章志翔; 周德俊; 邱宇杰; 王倩

    2001-01-01

    目的:探讨胃恶性肿瘤病人氨基酸代谢变化与肿瘤进展程度之间的关系,为胃恶性肿瘤氨基酸失衡疗法提供理论依据。 方法:采用Beckman-6300高效氨基酸自动分析仪,检测41例胃恶性肿瘤组织和癌旁胃粘膜组织中19种游离氨基酸的含量,对不同分期肿瘤组织氨基酸进行比较分析。 结果:与癌旁胃粘膜组织比较,胃恶性肿瘤组织中牛磺酸、苏氨酸、丝氨酸、脯氨酸、甘氨酸、丙氨酸、缬氨酸、异亮氨酸、亮氨酸、酪氨酸、赖氨酸含量升高(P<0.05)。进展期肿瘤与早期肿瘤相比,脯氨酸、缬氨酸、蛋氨酸、异亮氨酸、苯丙氨酸升高(P<0.05)。 结论:胃癌组织多种游离氨基酸含量显著高于癌旁胃粘膜组织,进展期胃恶性肿瘤组织某些氨基酸含量显著高于早期肿瘤组织。%Objectives:To determine the content of aminoacids in gastric cancer tissue and study the relationship between alterations of amino acids and cancer stages. Methods:19 free amino acids of cancer tissue and paracancerous normal mucose were determined in 41 cases of gastric cancer. Results:Most free amino acids were significantly increased in gastric cancer tissue as compared with those of paracancerous normal gastric tissue.The contents of proline,valine,methionine,isoleucine,leucine in advanced gastric cancer were significantly higher than those in early cases. Conclusions:Gastric tumor tissue contains high amount of most free amino acids particularly in cases with advanced cancer..

  17. Excreção urinária de 17-Cetoesteroides neutros no cavalo normal e no cavalo castrado

    Directory of Open Access Journals (Sweden)

    Fernando Ubatuba

    1954-06-01

    Full Text Available Total urinary neutral 17-steroids were determined in normal and in castrated horses. One liter of a 15-26 hours urine collection was hydrolysed by refluxing with 10% HC1 (v/v for ten minutes and extracted with peroxyde-free ethyl ether. The extract was purified by washing with saturated NaHCO³ and KOH solutions. One half of the crude neutral fraction was fractionated with Girard's "T" reagent . The Zimmermann reaction was performed both in the ketonic and in the crude neutral extracts, using alcoholic 2.5N KOH and a 60 minutes period for the colour development in the dark. Optical density measuments were made in a grating Coleman Universal Spectrophotometer at 420 mµ and 520mµ; for the crude neutral fraction a colour correction equation was applied. The aliquot fraction used for colorimety was adjusted for keeping optical density measurements within the range 0.2 to 0.7. Androsterone (mp. 184-184.5°C with an absorption maximum at 290.5 mµ (Beckman Model DU Spectrophotometer was used as a reference standard. Table I, ilustrates the results obtained. At the 0.05 probability level there is a significant difference among castrated and normal group means (Fischer's "t" test. when were used the data obtained from the ketonic fractions; in spite of the use of a colour correction applied for inespecific chromogens, the same results could not be obtained with the crude neutral fractions, Since Girard's reagent fractionation is generaly accepted as the best method for correcting the inespecific chromogen interference in the determination of the 17-ketosteroids by the Zimmermann reaction, we emphasize the value of the results obtained with the ketonic fractions. From these results it appears, as occurs in others mammals, that castrated horses show a lower level of urinary 17-ketosteroids excretion than the normal horses. The significance of the horse testis contribution for the neutral urinary steroid metabolites is discussed. Since horse urine has a

  18. Content Of 2,4-D-14C Herbicide Residue In Water And Soil Of Irrigated Rice Field System

    International Nuclear Information System (INIS)

    The investigation of 2,4-D exp.-14C herbicide residue in water and soil of irrigated rice field system was carried out. Rice plant and weeds (Monochoria vaginalis Burn. F. Presl) were planted in 101 buckets using two kinds of soil condition, I.e. normal soil and 30 % above normal compact soil. After one week planting, the plants were sprayed with 1 u Ci of 2,4-D exp.-14C and 0,4 mg non labeled 2,4-D. The herbicide residue content was determined 0, 2, 4, 8 and 10 weeks after spraying with 2,4-D herbicide. The analysis was done using Combustion Biological Oxidizer merk Harvey ox-400, and counted with Liquid Scintillation Counter merk Beckman model LS-1801. The results indicates that the herbicide contents in water and soil decrease from the first spraying with herbicide until harvest herbicide Residue content in water after harvest was 0.87 x 10 exp.-6 ppm for soil normal condition, and 0.59 x 10 exp.-6 pm for the soil 30 % up normal condition, while herbicide content in soil was 1.54 x 10 exp.-6 ppm for soil normal condition and 1.48 x 10 exp.-6 ppm for soil 30 % up normal. 2,4-D herbicide residue content in rice after harvest was 0.27 x 10 exp.-6 ppm for normal soil condition and 0.25 x 10 exp.-6 ppm for the soil 30 % up normal. 2,4-D herbicide residue content in roots and leaves of weeds after harvest were respectively 0.29 x 10 exp.-6 ppm and 0.18 x 10 exp.-6 for normal soil condition, while for 30 % up normal soil were 0.25 x 10 exp.-5 ppm and 0.63 x 10 exp.-7 ppm. This result indicates that there is no effect pollution to surrounding area, because the herbicide content is still bellow the allowed detection limit, 0.05 ppm

  19. Comparison of the argon tunable dye laser with the flashlamp pulsed dye laser in treatment of facial telangiectasia

    Science.gov (United States)

    Broska, Pamela; Martinho, Elena; Goodman, Matthew M.

    1992-06-01

    A prospective, side-by-side comparison study of two different lasers for the treatment of solar- induced telangiectasia was carried out in 14 patients at the Beckman Laser Institute and Medical Clinic. The argon tunable dye laser (Coherent, Palo Alto, Calif.) was used in the method modified from Orenstein and Nelson to completely treat discrete telangiectasias on one cheek. Specifically, the argon tunable dye laser (ATDL) was set at 0.7 - 0.8 watts, 585 nm wavelength, shutter-pulsed at 0.1 second duration with a spot size of 0.1 mm, and individual vessels were 'traced out' with 4X loupe magnification. Each patient's opposite cheek was then treated in the standard fashion with the flashlamp pulsed dye laser (Candela, Natick, Mass.) using a technique similar to Polla's et al. Specifically, the flashlamp pulsed dye laser (FPDL) was set at 585 nm wavelength, pulsed mode of 450 microseconds pulse duration, spot size of 5 mm, overlapping 10 - 20%, with power densities of 5.5 to 6.5 joules/cm2. All patients had symmetrical cheek telangiectasias of several years' duration. Patients were treated on day 0, and examined on weeks 2, 4, and 6. Photos were taken at each visit, and evaluation was done by questionnaire and direct observation, as well as by photographic slides later projected to an impartial panel. Final evaluation by the panel at week 6 showed 11/14 patients with excellent results (75 - 100% clearing) at sites treated with the FPDL, compared with 4/14 with the ATDL. In contrast, 4/14 FPDL sites were graded as fair to minimal improvement, and 9/14 as fair for the ATDL. The patients' self-evaluations graded the final results very similar to that of the panel. Most patients were bothered by the ecchymosis and hyperpigmentation associated with the FPDL, resulting in less than 50% of the patients preferring the FPDL despite its more impressive results. We conclude that the final results favor the FPDL over the ATDL for treatments of facial telangiectasia. However, non

  20. High performance capillary electrophoresis analysis of polypeptide in protein hydrolysis ingredients of brain tissue%脑蛋白水解物中多肽组分的高效毛细管电泳分析

    Institute of Scientific and Technical Information of China (English)

    王辰; 黄慧玲; 莫丽冬; 范维佳; 阎维维

    2012-01-01

    目的 利用高效毛细管电泳法比较2种水解方法提纯的脑蛋白水解物中的多肽组分,同时监测进口脑蛋白水解物注射液的多肽组分分布.方法 采用固相萃取法提取脑组织中的多肽成分,以PACE/A5500型高效毛细管电泳仪对进口脑蛋白水解物注射液和本实验室提取的脑组织匀浆物进行比较.结果 进口脑蛋白水解物注射液中多肽成分大部分集中在>14400区域,本实验室提纯的脑蛋白水解物成分中多肽多集中在相对分子质量6000 ~ 14 400区域,且纯度及含量均较高.结论 高效毛细管电泳法可有效地对脑蛋白水解物注射液中多肽组分进行分析和比较,2种提纯方法对脑蛋白的裂解能力相差不大.%Objective To extract the protein hydrolysis ingredients of brain tissue were extracted and to analyze the components of polypeptide in cerebrolysin by using the method of high-performance capillary electrophoresis (HPCE).Methods Brain homogenate were extracted by solid phase extraction method.Cerebrolysin and brain tissue hydrolyzated extracted in lab were detected by HPCE (PACE/A5500,Beckman).Result The result of HPCE showed that the cerebrolysin injections molecular weight of most polypeptides were more than 14 400.The purity and content of cerebrolysin was better and the molecular weights were mainly between 6000 and 14 400.Conclusion The method of HPCE can be effectively used in analyzing components of polypeptides of brain tissue hydrolyzated.There is no difference of protein hydrolysis ingredients of brain tissue extracted by 2 kinds of extraction methods.

  1. Antigen Expression on Blast Cells and Hematological Parameters at Presentation in Acute Lymphoblastic Leukemia Patients

    International Nuclear Information System (INIS)

    Objective: To analyze the expression of various antigens on the leukemic blasts and to determine the hematological parameters, in Acute Lymphoblastic Leukemia (ALL) patients at presentation. Study Design: Observational study. Place and Duration of Study: King Edward Medical University, Lahore and Hameed Latif Hospital, Lahore, from February 2013 to March 2014. Methodology: A total of 50 newly diagnosed and untreated patients of ALL were selected from Mayo Hospital and Hameed Latif Hospital. These patients included both genders and all age groups. Hemoglobin, total leukocyte count and platelet count were determined on hematology analyser-Sysmex-Kx-2I. Blast cell percentage was estimated on Giemsa stained blood smears. Immuno phenotyping was done on bone marrow samples by 5 colour flow cytometery on Beckman Counter Navious Flow cytometer. An acute leukemia panel of 23 antibodies was used. The data was entered and analyzed in SPSS version 22. Results: Of the 50 ALL patients, 36 (72 percentage) were B-ALL and 14 (28 percentage) T-ALL. There were 18 (36 percentage) children and 32 (64 percentage) adults. T-ALL included 22 percentage of the childhood and 31 percentage of the adult cases. Immuno phenotypic analysis showed that CD19, CD79a and CD20 were B-lineage specific markers whereas cCD3, CD3 and CD5 were T-lineage specific. CD10 was the most sensitive marker for B-ALL and CD7 was the most sensitive marker of T-ALL. TdT was expressed in 92 percentage B-ALL and 71 percentage T-ALL cases, CD34 in 58 percentage and 43 percentage cases and CD45 in 83 percentage and 100 percentage respectively. High leukocyte count (> 50 x 109/L) was present in 58 percentage cases. Hemoglobin was < 10 g/dl in 74 percentage patients and platelet count was below 20 x 109/Lin 12 percentage patients. Leukocyte count, hemoglobin, platelet count and blast cell percentage did not show a significant difference in the two ALL immuno types. Conclusion: The frequency of T-ALL is higher in childhood

  2. The effect of steam sterilization on the accuracy of spring-style mechanical torque devices for dental implants

    Directory of Open Access Journals (Sweden)

    Mahshid M

    2012-07-01

    Full Text Available Minoo Mahshid,1 Aboulfazl Saboury,1 Ali Fayaz,1 Seyed Jalil Sadr,1 Friedrich Lampert,2 Maziar Mir2,31Department of Prosthodontics, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2Department of Conservative Dentistry, Aachen RWTH Hospital, Aachen, Germany; 3Beckman Laser Institute, UCI, Irvine, CA, USABackground: Mechanical torque devices (MTDs are one of the most commonly recommended devices used to deliver optimal torque to the screw of dental implants. Recently, high variability has been reported about the accuracy of spring-style mechanical torque devices (S-S MTDs. Joint stability and survival rate of fixed implant supported prosthesis depends on the accuracy of these devices. Currently, there is limited information on the steam sterilization influence on the accuracy of MTDs. The purpose of this study was to assess the effect of steam sterilization on the accuracy (±10% of the target torque of spring-style mechanical torque devices for dental implants.Materials and methods: Fifteen new S-S MTDs and their appropriate drivers from three different manufacturers (Nobel Biocare, Straumann [ITI], and Biomet 3i [3i] were selected. Peak torque of devices (5 in each subgroup was measured before and after autoclaving using a Tohnichi torque gauge. Descriptive statistical analysis was used and a repeated-measures ANOVA with type of device as a between-subject comparison was performed to assess the difference in accuracy among the three groups of spring-style mechanical torque devices after sterilization. A Bonferroni post hoc test was used to assess pairwise comparisons.Results: Before steam sterilization, all the tested devices stayed within 10% of their target values. After 100 sterilization cycles, results didn't show any significant difference between raw and absolute error values in the Nobel Biocare and ITI devices; however the results demonstrated an increase of error values in the 3i group (P < 0.05. Raw error

  3. The multi-xenobiotic resistance (MXR) efflux activity in hemocytes of Mytilus edulis is mediated by an ATP binding cassette transporter of class C (ABCC) principally inducible in eosinophilic granulocytes.

    Science.gov (United States)

    Rioult, Damien; Pasquier, Jennifer; Boulangé-Lecomte, Céline; Poret, Agnès; Abbas, Imane; Marin, Matthieu; Minier, Christophe; Le Foll, Frank

    2014-08-01

    In marine and estuarine species, immunotoxic and/or immunomodulatory mechanisms are the crossroad of interactions between xenobiotics, microorganisms and physicochemical variations of the environment. In mussels, immunity relies exclusively on innate responses carried out by cells collectively called hemocytes and found in the open hemolymphatic circulatory system of these organisms. However, hemocytes do not form a homogenous population of immune cells since distinct subtypes of mussel blood cells can be distinguished by cytochemistry, flow cytometry or cell motility analysis. Previous studies have also shown that these cells are able to efflux xenobiotics by means of ATP binding cassette (ABC) transporter activities conferring a multixenobiotic resistance (MXR) phenotype. ABC transporters corresponding to vertebrate class B/P-glycoprotein (P-gp) and to class C/multidrug resistance related protein (MRP) are characterized in Mytilidae. Herein, we have investigated the relative contributions of ABCB- and ABCC-mediated efflux within the different hemocyte subpopulations of Mytilus edulis mussels, collected from areas differentially impacted by chemical contaminants in Normandy (France). RT-PCR analyses provide evidence for the presence of ABCB and ABCC transporters transcripts in hemocytes. Immunodetection of ABCB/P-gp with the monoclonal antibody UIC2 in living hemocytes revealed that expression was restricted to granular structures of spread cells. Efflux transporter activities, with calcein-AM as fluorescent probe, were measured by combining flow cytometry to accurate Coulter cell size measurements in order to get a cell-volume normalized fluorescence concentration. In these conditions, basal fluorescence levels were higher in hemocytes originating from Yport (control site) than in cells collected from the harbor of Le Havre, where mussels are more exposed to with persistent pollutants. By using specific ABCB/P-gp (verapamil, PSC833, zosuquidar) and ABCC/MRP (MK

  4. Does chronic alcohol use by HIV-infected patients on d4T/3TC/NVP drug regimen effect the HIV viral load and what is the therapeutic window of the drugs, CD4+ count and WBC count in patients with high viral load during the 9 months period of follow up?

    Directory of Open Access Journals (Sweden)

    Godfrey S. Bbosa

    2013-10-01

    Full Text Available The study investigated the effects of chronic alcohol use on HIV viral load in HIV-infected patients on d4T/3TC/NVP drug regimen during 9 months follow up period. It also determined plasma drug concentrations of d4T, 3TC and NVP; CD4+ and WBC counts for patients with high HIV viral load. A case-control study using repeated measures with serial measurements was used. A total of 41 patients (20 alcohol group and 21 control group were screened for alcohol use using WHO AUDIT tool and chronic alcohol use biomarkers. Blood sampling was done at 3 month intervals for a period of 9 months. HIV viral load was determined using Roche Amplicor HIV-1 monitor test, version 1.5 (Amplicor. The d4T, 3TC and NVP concentrations were determined by Shimadzu Class-VPTM HPLC Chromatography data system version 6.1. The CD4+ cell count was determined using FACSCalibur flow cytometer. The WBC was determined using automated hematological Coulter CBC-5 Hematology Analyzer system. Results show that % patients with HIV viral load ≥400 copies/ml in control group was highest (23.8%, n=5 at 3 month while in chronic alcohol use group, it was at 0 month (35%, n=7 for both WHO AUDIT tool and chronic alcohol-use biomarkers groups. Generally patients with high viral load ≥400 copies/ml was observed in chronic alcohol use as compared to control group in both WHO AUDIT tool and biomarkers group despite of patients having high steady state d4T, 3TC and NVP plasma drug concentrations in circulation that is available to suppress HIV virus. The high viral load could be associated with the emergence of resistance of the HIV virus and these patients generally had a low CD4+ cell count. Some of these patients had no detectable d4T plasma drug concentrations in circulation and most of them with high viral load had sub-therapeutic NVP plasma drug concentrations in their blood circulation. Chronic ethanol use by HIV-infected patients on d4T/3TC/NVP drug regimen increased HIV viral load and

  5. Effect of chronic alcohol consumption on the red blood cell count and RBC indices in the HIV infected patients on d4T/3TC/NVP drug regimen in Uganda

    Directory of Open Access Journals (Sweden)

    Godfrey S. Bbosa

    2013-10-01

    Full Text Available Alcohol consumption is common problem in Uganda. Among the types of alcohols consumed include beers, spirits, liqueurs, wines and traditional brew. These alcohols are easily accessible and consumed by many people including the HIV infected patients who are on the d4T/3TC/NVP regimen. The aim of this study was to determine the effect of chronic alcohol intake on the red blood cell count (RBC and the RBC indices in the HIV-infected patients on d4T/3TC/NVP regimen. It was a case control study that used a repeated measures design model where serial measurements of the red blood cell count (RBC and RBC indices were determined at 3 month interval for 9 months. A total of 41 HIV infected patients were recruited and grouped into two arms; the control group had 21 patients and the chronic alcohol group had 20 patients. The RBC and RBC indices of the whole blood were determined using automated hematological Coulter CBC-5 Hematology Analyzer system using standard procedures. The data was sorted into alcohol-use self reporting by WHO AUDIT tool and alcohol-use biomarkers groups. It was analysed using the SAS 2003 version 9.1 statistical package with the repeated measures fixed model. The means were compared using the student t-test. The mean MCV and MCH values in the chronic alcohol use group were higher than in the control group and there was a significant difference between the 2 groups (p<0.05 for both the WHO AUDIT tool group and chronic alcohol use biomarkers group. The mean RBC count, Hct, HGB and MCHC values in both the control and chronic alcohol use groups were within the normal reference ranges for both groups though the trend was lower in alcohol group. Chronic alcohol use affects the RBC and RBC indices in the HIV infected patients on d4T/3TC/NVP treatment regimen. [Int J Basic Clin Pharmacol 2013; 2(5.000: 528-536

  6. Cytometric evaluation of intracellular IFN-γ and IL-4 levels in thyroid follicular cells from patients with autoimmune thyroid diseases

    Directory of Open Access Journals (Sweden)

    Bossowski Artur

    2011-09-01

    Full Text Available Abstract Background In recent few years is underlined that altered balance of pro- and anti-inflammatory cytokines play an important role in the pathogenesis of AITD. The aim of this study was to estimate intracellular INF-γ and IL-4 levels in thyroid-infiltrating lymphocytes and thyrocytes isolated from thyroid tissues in 54 adolescent patients aged 8-21 years, with Graves' disease (GD; n = 18, Hashimoto's thyroiditis (HT; n = 18 and non-toxic multinodular goiter (NTMG; n = 18. Methods Fresh thyroid tissues were taken on culture medium RPMI -1640, it was mechanically prepared. In next step were added cell activators -12- myristate 13- the acetate (PMA and Ionomycin as well as the inhibitor of transportation of proteins - Breferdin A. They were cultured 24 hours in 50 ml flasks at 37°C in a 5-95% CO2-air water-saturated atmosphere. After that, thyrocytes were identified by mouse mAb directed against human TPO epitope 64 conjugated with rabbit anti-mouse antibodies IgG (Fab'2 labeled by FITC. After incubation at room temperature to each of samples added reagent A fixative the cellular membrane. In next step into the cell suspensions were added reagent B to permeabilization of cellular membrane and specific anti-IL-4-PE or anti-IFN-γ-PE mAbs. Identification of intracellular cytokines in T lymphocytes was performed in the same procedure with application of anti-CD4-PerCP and anti-CD8-PerCP mAbs specific for T lymphocytes. The cells were analyzed in a flow cytometry (Coulter EPICS XL. Results In examined group of patients with GD we observed statistically significant higher mean percentage of cells with phenotype CD4+IL-4 (p Conclusions We conclude that human thyrocytes in autoimmune thyroid disorders could be a source of cytokine production and that their activation influences local interaction with T lymphocytes inflowing to the thyroid gland.

  7. The Long-Term Conditions Questionnaire: conceptual framework and item development

    Directory of Open Access Journals (Sweden)

    Peters M

    2016-08-01

    Full Text Available Michele Peters,1 Caroline M Potter,1 Laura Kelly,1 Cheryl Hunter,1 Elizabeth Gibbons,1 Crispin Jenkinson,1 Angela Coulter,1 Julien Forder,2 Ann-Marie Towers,2 Christine A’Court,3,4 Ray Fitzpatrick1 1Health Services Research Unit, Nuffield Department of Population Health, University of Oxford, Oxford, 2Personal Social Services Research Unit, University of Kent, Canterbury, 3Nuffield Department of Primary Health Care Sciences, University of Oxford, Oxford, 4Broadshires Health Centre, Carterton, UK Purpose: To identify the main issues of importance when living with long-term conditions to refine a conceptual framework for informing the item development of a patient-reported outcome measure for long-term conditions.Materials and methods: Semi-structured qualitative interviews (n=48 were conducted with people living with at least one long-term condition. Participants were recruited through primary care. The interviews were transcribed verbatim and analyzed by thematic analysis. The analysis served to refine the conceptual framework, based on reviews of the literature and stakeholder consultations, for developing candidate items for a new measure for long-term conditions.Results: Three main organizing concepts were identified: impact of long-term conditions, experience of services and support, and self-care. The findings helped to refine a conceptual framework, leading to the development of 23 items that represent issues of importance in long-term conditions. The 23 candidate items formed the first draft of the measure, currently named the Long-Term Conditions Questionnaire.Conclusion: The aim of this study was to refine the conceptual framework and develop items for a patient-reported outcome measure for long-term conditions, including single and multiple morbidities and physical and mental health conditions. Qualitative interviews identified the key themes for assessing outcomes in long-term conditions, and these underpinned the development of the

  8. COMBINED PRIMARY TILLAGE AND COMPARATIVE ENERGY EVALUATION OF DIFFERENT TECHNOLOGICAL PROCESSES OF SOIL TREATMENT

    Directory of Open Access Journals (Sweden)

    Zhuchenko A. V.

    2016-02-01

    Full Text Available Plowing is the main and most energy-intensive way of primary tillage. The development and the introduction of the energy-saving ways of the primary tillage are of great scientific and practical interest. In the Azov-Black Engineering Institute, we have developed a combined method of the tillage and a tool using left-hand blades designed to handle the lower layers of the soil. The tool is designed for a layered tillage providing moldboard shallow and mouldboardless shallow (12 ... 18 cm subsurface tillage using the cropping and the offset of the lower layer of soil in the direction of the daylight surface. The installation of the left-hand blades is designed to decrease the energy intensity of the technological process by reducing the weight of soil treated by the plow body. The aim of this study is to compare the energy assessment of the three ways of the tillage: - Plowing without skimmers; - Plowing with coulters being at the wall of the furrow; - Plowing with a left-hand blades tool. The study has been carried out using a graphical simulation of the shifting of the center of gravity of the soil layer. The assessment of the energy intensity of the technological process has been carried out by the capacity value consumed to shift the center of gravity of the treated layer of soil at the different depths of the tillage. The results of the study have showed that the change of the total quantity of the shifting of the center of gravity of the treated soil layer ranges from 0.43 ... to 0.67 m in respect of the two first-mentioned ways of plowing, while the same index in respect of the combined tool ranges from 0.37 ...to 0.55 m. The maximum power consumption of the process is also higher in respect of the two first-mentioned ways of the tillage. The power required to shift the center of gravity of the treated layer in respect of both ways ranges from 0.6 ... to 2.0 kW. , while the same index in respect of the combined method of tillage ranges from

  9. The multi-xenobiotic resistance (MXR) efflux activity in hemocytes of Mytilus edulis is mediated by an ATP binding cassette transporter of class C (ABCC) principally inducible in eosinophilic granulocytes.

    Science.gov (United States)

    Rioult, Damien; Pasquier, Jennifer; Boulangé-Lecomte, Céline; Poret, Agnès; Abbas, Imane; Marin, Matthieu; Minier, Christophe; Le Foll, Frank

    2014-08-01

    In marine and estuarine species, immunotoxic and/or immunomodulatory mechanisms are the crossroad of interactions between xenobiotics, microorganisms and physicochemical variations of the environment. In mussels, immunity relies exclusively on innate responses carried out by cells collectively called hemocytes and found in the open hemolymphatic circulatory system of these organisms. However, hemocytes do not form a homogenous population of immune cells since distinct subtypes of mussel blood cells can be distinguished by cytochemistry, flow cytometry or cell motility analysis. Previous studies have also shown that these cells are able to efflux xenobiotics by means of ATP binding cassette (ABC) transporter activities conferring a multixenobiotic resistance (MXR) phenotype. ABC transporters corresponding to vertebrate class B/P-glycoprotein (P-gp) and to class C/multidrug resistance related protein (MRP) are characterized in Mytilidae. Herein, we have investigated the relative contributions of ABCB- and ABCC-mediated efflux within the different hemocyte subpopulations of Mytilus edulis mussels, collected from areas differentially impacted by chemical contaminants in Normandy (France). RT-PCR analyses provide evidence for the presence of ABCB and ABCC transporters transcripts in hemocytes. Immunodetection of ABCB/P-gp with the monoclonal antibody UIC2 in living hemocytes revealed that expression was restricted to granular structures of spread cells. Efflux transporter activities, with calcein-AM as fluorescent probe, were measured by combining flow cytometry to accurate Coulter cell size measurements in order to get a cell-volume normalized fluorescence concentration. In these conditions, basal fluorescence levels were higher in hemocytes originating from Yport (control site) than in cells collected from the harbor of Le Havre, where mussels are more exposed to with persistent pollutants. By using specific ABCB/P-gp (verapamil, PSC833, zosuquidar) and ABCC/MRP (MK

  10. Role of Tamm-Horsfall protein and uromodulin in calcium oxalate crystallization

    Directory of Open Access Journals (Sweden)

    Carvalho M.

    2002-01-01

    Full Text Available One of the defenses against nephrolithiasis is provided by macromolecules that modulate the nucleation, growth, aggregation and retention of crystals in the kidneys. The aim of the present study was to determine the behavior of two of these proteins, Tamm-Horsfall and uromodulin, in calcium oxalate crystallization in vitro. We studied a group of 10 male stone formers who had formed at least one kidney stone composed of calcium oxalate. They were classified as having idiopathic nephrolithiasis and had no well-known metabolic risk factors involved in kidney stone pathogenesis. Ten normal men were used as controls, as was a group consisting of five normal women and another consisting of five pregnant women. Crystallization was induced by a fixed supersaturation of calcium oxalate and measured with a Coulter Counter. All findings were confirmed by light and scanning electron microscopy. The number of particulate material deposited from patients with Tamm-Horsfall protein was higher than that of the controls (P<0.001. However, Tamm-Horsfall protein decreased the particle diameter of the stone formers when analyzed by the mode of the volume distribution curve (P<0.002 (5.64 ± 0.55 µm compared to 11.41 ± 0.48 µm of uromodulin; 15.94 ± 3.93 µm and 12.45 ± 0.97 µm of normal men Tamm-Horsfall protein and uromodulin, respectively; 8.17 ± 1.57 µm and 9.82 ± 0.95 µm of normal women Tamm-Horsfall protein and uromodulin, respectively; 12.17 ± 1.41 µm and 12.99 ± 0.51 µm of pregnant Tamm-Horsfall protein and uromodulin, respectively. Uromodulin produced fewer particles than Tamm-Horsfall protein in all groups. Nonetheless, the total volume of the crystals produced by uromodulin was higher than that produced by Tamm-Horsfall protein. Our results indicate a different effect of Tamm-Horsfall protein and uromodulin. This dual behavior suggests different functions. Tamm-Horsfall protein may act on nucleation and inhibit crystal aggregation, while

  11. 不同品牌化学发光仪检测甲状腺激素的相关性分析

    Institute of Scientific and Technical Information of China (English)

    谭云昌; 曾正莲

    2010-01-01

    目的 探讨两台不同品牌化学发光免疫分析仪检测结果的一致性.方法 每天患者标本采用Bechman Coulter ACCESS2检测后,根据仪器的游离三碘甲腺原氨酸(FT3)正常参考范围选择正常和异常各一标本采用Abbott Axsm Symtem进行检测,正常、异常标本各30份,记录用两种方法检测的数据.结果 30份FT3正常标本和30份FT3异常标本分别在2个不同检测系统测定结果方差齐,经配伍组设计资料的方差分析,各组间的差异有统计学意义(P<0.05),可靠性系数@=0.990,2个检测系统间FT3、游离甲状腺素(FT4)、促甲状腺素(TSH)的相关系数均大于0.970.结论 两台不同仪器间的测定结果差异有统计学意义,通过选择一台参加湖北省临床检验中心和卫生部临床检验中心内分泌项目获得室间质评成绩优秀的仪器作为参考仪器,配制出与另外一台仪器定标液浓度相同的血清作为校准品来校正另外一台仪器的方法,结果表明,两台仪器测定患者标本结果差异无统计学意义,比对试验符合临床要求.

  12. Cell type-dependent uptake, localization, and cytotoxicity of 1.9 nm gold nanoparticles

    Directory of Open Access Journals (Sweden)

    Coulter JA

    2012-06-01

    Full Text Available Jonathan A Coulter,1 Suneil Jain,2 Karl T Butterworth,2 Laura Taggart,2 Glenn Dickson,2 Stephen J McMahon,3 Wendy Hyland,1 Mark F Muir,3 Coleman Trainor,2 Alan Hounsell,2,4 Joe M O'Sullivan,2,4 Giuseppe Schettino,2 Fred Currell,3 David G Hirst,1 Kevin M Prise21School of Pharmacy, McClay Research Centre, 2Centre for Cancer Research and Cell Biology, 3School of Mathematics and Physics, Queens University Belfast, 4Belfast Health and Social Care Trust, Belfast, IrelandBackground: This follow-up study aims to determine the physical parameters which govern the differential radiosensitization capacity of two tumor cell lines and one immortalized normal cell line to 1.9 nm gold nanoparticles. In addition to comparing the uptake potential, localization, and cytotoxicity of 1.9 nm gold nanoparticles, the current study also draws on comparisons between nanoparticle size and total nanoparticle uptake based on previously published data.Methods: We quantified gold nanoparticle uptake using atomic emission spectroscopy and imaged intracellular localization by transmission electron microscopy. Cell growth delay and clonogenic assays were used to determine cytotoxicity and radiosensitization potential, respectively. Mechanistic data were obtained by Western blot, flow cytometry, and assays for reactive oxygen species.Results: Gold nanoparticle uptake was preferentially observed in tumor cells, resulting in an increased expression of cleaved caspase proteins and an accumulation of cells in sub G1 phase. Despite this, gold nanoparticle cytotoxicity remained low, with immortalized normal cells exhibiting an LD50 concentration approximately 14 times higher than tumor cells. The surviving fraction for gold nanoparticle-treated cells at 3 Gy compared with that of untreated control cells indicated a strong dependence on cell type in respect to radiosensitization potential.Conclusion: Gold nanoparticles were most avidly endocytosed and localized within cytoplasmic

  13. Desempenho da semeadora-adubadora MAGNUM 2850 em plantio direto no basalto paranaense Performance of MAGNUM 2850 seeder in nontillage in the basaltic soil of Paraná, Brazil

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    RUY CASÃO JUNIOR

    2000-03-01

    Full Text Available O sistema de plantio direto atingiu, no Estado do Paraná, cerca de dois milhões de hectares, nos últimos anos, e um dos seus principais entraves é a falta de semeadoras-adubadoras apropriadas para operação em solos argilosos e de origem basáltica. Portanto, estudou-se a MAGNUM 2850 PD quanto à demanda energética e desempenho operacional, com a finalidade de oferecer subsídios ao seu aperfeiçoamento, de acordo com a realidade paranaense. Observou-se que o esforço de tração variou em função da umidade e características mecânicas do solo, atingiu valores elevados, e exigiu, em situações extremas, potência de até 79,5 kW do trator. Os componentes de ataque ao solo mostraram um bom desempenho no corte da vegetação e na uniformidade da profundidade de trabalho, porém apresentaram grande área mobilizada no sulco. Obteve-se boa qualidade de semeadura, avaliada pela porcentagem e velocidade de emergência das sementes no campo. Foram identificados erros de dosagem do fertilizante e deposição das sementes, e principalmente na velocidade de deslocamento de 8,0 km/h; porém, foi boa a uniformidade transversal das sementes e plantas no campo.The nontillage system has attained two million hectars in the State of Paraná, Brazil, in the last years, but one of the most important problems was the lack of suitable seeders to basaltic clay soils. Therefore, MAGNUM 2850 PD was studied as for energetic requirement and operational performance in order to fit it to the reality of the State of Paraná. It was observed that the traction power varied in function of moisture and of mechanical characteristics of the soil, and attained high values and high power demand in maximal situations -- around 79.5 kW of the tractor. The soil coulter openers showed a good performance in cutting the vegetal and in depth uniformity of work; but they also showed a big mobilized area in the furrow. Good sowing quality was obtained, which was evaluated by the

  14. Physical and Chemical Characteristics of Desert Dust Deposited on Mt. Elbrus, Caucasus as Documented in Snow Pit and Shallow Core Records

    Science.gov (United States)

    Kutuzov, S.; Shahgedanova, M.; Mikhalenko, V.; Ginot, P.; Lavrentiev, I.; Popov, G.

    2013-12-01

    We present a study of dust deposition events and its physical and chemical characteristics in Caucasus Mountains as documented by snow and firn pack at Mt Elbrus. Dust samples were collected from the shallow ice cores and snow pits in 2009-2013 at the western Elbrus plateau (5150 m a.s.l.). Particle size distribution and chemical analysis (major ions, trace elements) were completed for each sample using Coulter Counter Multisizer III, scanning electron microscopy (SEM), IC and ICPMS analysis. It was shown that desert dust deposition occurred in Caucasus 4-8 times a year and originates from the Northern Sahara and the deserts of the Middle East. Analysis of volumetric particle size distributions showed that the modal values ranged between 2 μm and 4 μm although most samples were characterised by modal values of 2.0-2.8 μm with an average of 2.6 μm. These values are lower than those obtained from the ice cores in central and southern Asia following the deposition of long-travelled dust and are closer to those reported for the European Alps and the polar ice cores. All samples containing dust have a single mode which is usually interpreted as a single source region. They do not reveal any significant differences between the Saharan and the Middle Eastern sources. The annual average dust mass concentrations were 10-15 mg kg-1 which is higher than the average concentrations reported for other mountain regions and this was strongly affected by dust deposition events. The deposition of dust resulted in elevated concentrations of most ions, especially Ca2+, Mg2+, K+, and sulphates. Dust originated from multiple sources in the Middle East including Mesopotamia or passing over the Middle East was characterised by the elevated concentrations of nitrates and ammonia which is related to a high atmospheric loads of ammonium emitted by agricultural sources and high concentrations of ammonium in dust originating from this region. By contrast, samples of the Saharan dust showed

  15. Effectiveness of a physiotherapy-initiated telephone triage of orthopedic waitlist patients

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    Gilmore L

    2011-08-01

    Full Text Available Joanne Morris1, Karen Grimmer-Somers2, Saravana Kumar2, Karen Murphy3, Lisa Gilmore1, Bryan Ashman1, Chandima Perera1, Kathryn Vine1, Corinne Coulter11The Canberra Hospital, ACT Government Health Directorate, Canberra, ACT, Australia; 2International Centre for Allied Health Evidence, University of South Australia, Adelaide, SA, Australia; 3ACT Government Health Directorate, Canberra, ACT, AustraliaBackground: There is generally a lengthy wait on outpatient orthopedic waiting lists in Australian public hospitals to consult a specialist. Patients then wait again for surgery, if required. Patients with higher need are rarely prioritized, and there is the potential for increased morbidity for those who wait. There is generally no option of alternative care whilst waiting. This paper compares historical orthopedic outpatient clinic data with the outcomes of a physiotherapy-led initiative in one large Australian tertiary hospital.Methods: Two physiotherapists working within-scope conducted a telephone triage (October to December 2010 using a standard instrument for all new patients on the orthopedic waiting list. They were offered primary treatment options of retaining their appointment, being discharged, referral to a new model of assessment (multidisciplinary specialist clinic, or referral to physiotherapy. The outcomes were costs of the service, waiting time, and percentage of patients taking up management options. This was compared with a historical sample of new patients on the orthopedic waiting list (January to March 2009, whose treatment consumption was tracked longitudinally.Results: The telephone triage resulted in 16.4% patients being discharged directly (compared with 0.1% comparison sample. For approximately AU$17.00 per patient, the telephone triage process released 21 booked appointments on the outpatient clinic waiting list. Moreover, approximately 26% patients were referred directly to physiotherapy, which was not a primary management

  16. Mechanical damage of red blood cells by rotary blood pumps: selective destruction of aged red blood cells and subhemolytic trauma.

    Science.gov (United States)

    Sakota, Daisuke; Sakamoto, Ryuki; Sobajima, Hideo; Yokoyama, Naoyuki; Waguri, Satoshi; Ohuchi, Katsuhiro; Takatani, Setsuo

    2008-10-01

    In this study, mean cell volume (MCV), mean cell hemoglobin concentration (MCHC), and mean cell hemoglobin (MCH) were measured to quantify RBC damage by rotary blood pumps. Six-hour hemolysis tests were conducted with a Bio-pump BPX-80, a Sarns 15200 roller pump, and a prototype mag-lev centrifugal pump (MedTech Heart) using fresh porcine blood circulated at 5 L/min against a 100 mm Hg head pressure. The temperature of the test and noncirculated control blood was maintained at 37 degrees C. The normalized index of hemolysis (NIH) of each pump was determined by measuring the plasma-free hemoglobin level. The MCV was measured with a Coulter counter, and MCHC was derived from total hemoglobin and hematocrit. MCH was derived from MCV and MCHC. A multivariance statistical analysis (ANOVA) revealed statistically significant differences (n = 15, P < 0.05) in MCV, MCHC, and MCH between the blood sheared by the rotary blood pumps and the nonsheared control blood. Normalized to the control blood, the Bio-pump BPX-80 showed an MCV of 1.04 +/- 0.03, an MCHC of 0.95 +/- 0.04, and an MCH of 0.98 +/- 0.02; the mag-lev MedTech Heart had an MCV of 1.02 +/- 0.02, an MCHC of 0.97 +/- 0.02, and an MCH of 0.99 +/- 0.01; and the roller pump exhibited an MCV of 1.03 +/- 0.03, an MCHC of 0.96 +/- 0.03, and an MCH of 0.99 +/- 0.01. Per 0.01 increase in NIH, the BPX-80 showed a normalized MCV change of +10.1% and a normalized MCHC change of -14.0%; the MedTech Heart demonstrated a +6.9% MCV and -9.5% MCHC change; and the roller pump had a +0.5% MCV and -0.6% MCHC change. Due to shear in the pump circuits, the RBC increased while the MCHC decreased. The likely mechanism is that older RBCs with smaller size and higher hemoglobin concentration were destroyed fast by the shear, leaving younger RBCs with larger size and lower hemoglobin concentration. Subhemolytic trauma caused the intracellular hemoglobin to decrease due to gradual hemoglobin leakage through the micropores formed in the thinned

  17. First cytochemical study of haemocytes from the crab Carcinus aestuarii (Crustacea, Decapoda

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    V. Matozzo

    2010-03-01

    Full Text Available For the first time, a morphological study of haemocytes from the crab Carcinus aestuarii was carried out by means of light microscopy and differing cytochemical assays. Analysis of haemocyte size frequency distribution (performed by means of a Coulter Counter revealed the presence of two distinct haemocyte fractions in C. aestuarii haemolymph, depending on cell size. The first fraction was of about 3-5 µm in diameter and 30-50 fL in volume, the second was of about 6-12 µm in diameter and over 200 fL in volume. Mean cell diameter and volume were 8.20±1.7 µm and 272.30±143.5 fL, respectively. Haemocytes observed under light microscope were distinguished in three cell types: granulocytes (28%; 11.94±1.43 µm in diameter with evident cytoplasmic granules, semigranulocytes (27%; 12.38±1.76 µm in diameter with less granules than granulocytes, and hyalinocytes (44%; 7.88±1.6 µm in diameter without granules. In addition, a peculiar cell type was occasionally found (about 1%: it was 25-30 µm in diameter and had a great vacuole and a peripheral cytoplasm with granules. Granulocyte and semigranulocyte granules stained in vivo with Neutral Red, indicating that they were lysosomes. Giemsa’s dye confirmed that granulocytes and semigranulocytes were larger than hyalinocytes. Pappenheim’s panoptical staining and Ehrlich’s triacid mixture allowed to distinguish granule-containing cells (including semigranulocytes in acidophils (64%, basophils (35% and neutrophils (1%. Hyalinocytes showed always a basophilic cytoplasm. Haemocytes were positive to the PAS reaction for carbohydrates, even if cytoplasm carbohydrate distribution varied among cell types. Lastly, lipids were found on cell membrane and in cytoplasm of all haemocyte types in the form of black spots produced after Sudan Black B staining. The morphological characterisation of C. aestuarii haemocytes by light microscopy was necessary before performing both ultrastructural and functional

  18. Interstitial diffuse radiance spectroscopy of gold nanocages and nanorods in bulk muscle tissues

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    Grabtchak S

    2015-02-01

    Full Text Available Serge Grabtchak,1,2 Logan G Montgomery,1 Bo Pang,3,4 Yi Wang,4,5 Chao Zhang,6,7 Zhiyuan Li,6,7 Younan Xia,4,8 William M Whelan1,91Department of Physics, University of Prince Edward Island, Charlottetown, PEI, Canada; 2Departments of Electrical and Computer Engineering, and Physics, Dalhousie University, Halifax, Canada; 3Department of Biomedical Engineering, Peking University, Beijing, People’s Republic of China; 4The Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA; 5Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing, People’s Republic of China; 6Laboratory of Optical Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, People’s Republic of China; 7College of Physics and Optoelectronics, South China University of Technology, Guangzhou, People’s Republic of China; 8School of Chemistry and Biochemistry, and School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, USA; 9Atlantic Veterinary College, Charlottetown, PEI, CanadaAbstract: Radiance spectroscopy was applied to the interstitial detection of localized inclusions containing Au nanocages or nanorods with various concentrations embedded in porcine muscle phantoms. The radiance was quantified using a perturbation approach, which enabled the separation of contributions from the porcine phantom and the localized inclusion, with the inclusion serving as a perturbation probe of photon distributions in the turbid medium. Positioning the inclusion at various places in the phantom allowed for tracking of photons that originated from a light source, passed through the inclusion’s location, and reached a detector. The inclusions with high extinction coefficients were able to absorb nearly all photons in the range of 650–900 nm, leading to a spectrally flat radiance signal. This signal could be

  19. Science education through open and distance learning at Higher Education level

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    Amrita NIGAM

    2007-10-01

    Full Text Available Abstract The changes faced by the society in the past few decades brought revolution in all areas. The job requirements have undergone change tremendously. The emergence of e-culture, e-education, e-governance, e-training, e-work sites and so on questioned the capacity of conventional face to face education in catering to all and relevance of existing job related skills to a great extent in the emerging global society. Today, every one has to update his/her educational and/or professional skills and competencies to cope up with the emerging work challenges. This is more so in the field of science and technology. At the same time, it is impossible to cater to educational and training opportunities to one and all those who aspire for it through the conventional set up. The distance and open learning (ODL seems to be one of the viable alternatives. Today, the success and viability of ODL is accepted globally. Coulter (1989, through a study demonstrated that ODL is a cost-effective medium in providing educational opportunities. Similarly Holmberg (1981 also mentioned ODL as a systematic teaching-learning medium by using variety of medium for imparting learning. The present study is an attempt to study the experiences of the open science learners of IGNOU on different aspect of the science higher education. Here a questionnaire was used to collect the data and responses from 81 students enrolled for B. Sc. from IGNOU were collected. The findings of the study reported that society has undergone drastic changes in the last few decades. The revolution led due to Information and Communication Technologies (ICTs have widely affected all aspects of society. The emerging jobs require entirely new skills and competencies i.e., employment in BPOs or switching over to e-governance, e-Banking and e- based sectors. Even e-learning has made numerous expectations from teachers and other personnel. The use of ICTs in almost every field needs adequately trained

  20. Analysis on causes and countermeasures on pseudo - reduction of platelet count by blood cell analyzer%血细胞分析仪计数血小板假性减少原因分析及对策探讨

    Institute of Scientific and Technical Information of China (English)

    李惠卿; 赖馨; 蔡洁丹; 高洪丽; 唐万兵

    2014-01-01

    Objective To explore the causes and treatment strategies for pseudo - reduction of platelet count by blood cell analyzer. Methods A total of 351 blood samples for platelet count less than 100 × 109 / L measured by Beckman LH780 automatic blood cell analyzer were re - examined by handmade dilute count and smear staining for microscopy. They were divided into 5 groups according to platelet histogram,297 specimens with normal platelet histogram were allocated in group A;13 specimens with small platelet histogram in group B:11 specimens with large platelet histogram in group C:19 specimens with platelet aggregation histogram in group D,and 11 specimens with red cell fragments histo-gram in group E. The platelet count by blood cell analyzer and handmade dilute count were compared with smear staining examination under mi-croscopy for degree of coincidence. Results ① In comparison with handmade dilute count,the difference between group A and group B was not statistically significant( P ﹥ 0. 05),the difference between group C and group D was statistically significant( P < 0. 01),and the platelet count with blood cell analyzer was higher in group E,and the difference between group E and other groups was statistically significant( P < 0. 01). ②There was high degree of coincidence in results between handmade dilution count and smear staining examination under microscopy . Conclusion Platelet count by blood cell analyzer may be pseudo - reduced. In order to raise the accuracy of platelet count,the samples with abnormal platelet histogram need to be checked by handmade dilute count and smear staining examination under microscopy.%目的:探讨血细胞分析仪计数血小板(PLT)假性减少的原因及处理对策。方法对 Beckman LH780自动血细胞分析仪计数 PLT <100×109/ L 的351例样本进行手工稀释计数及涂片染色镜检,按血小板直方图类型分组,A组(正常血小板直方图):297例;B 组

  1. Atividade elétrica cerebral do rato com lesões da formação reticular mesencefálica Electrocorticographic study of the rats's bram after lesioning of the midbrain reticular formation

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    Walter C. Pereira

    1970-09-01

    Full Text Available No presente estudo foram utilizados 73 ratos em preparações agudas e crônicas, nas quais lesamos a formação reticular mesencefálica com corrente contínua (3,5 a 4,0 mA durante 5 a 10 segundos. O eletródio ativo era implantado estereotàxicamente segundo as coordenadas de König e Klippel. As lesões eram feitas parcial ou totalmente, uni ou bilateralmente, e em todos os animais procedeu-se ao controle histológico das áreas lesadas, usando-se o método de Weil. O registro da atividade elétrica cortical foi feito com polígrafo Beckman de 4 canais, utilizando-se derivações bipolares curtas (1mm com eletródios esféricos de platina. As experiências permitiram as seguintes conclusões: 1 — As características eletrofisiológicas dos fusos que ocorrem após lesões da formação reticular mesencefálica são muito semelhantes às dos fusos espontâneos e barbitúricos, inclusive quanto à projeção cortical. Quanto à duração dos potenciais que os constituem, contudo, notamos que a faixa de variação era mais centuada (20 a 80 ms, o que pode ser atribuído à maior complexidade dos potenciais do cérebro isolado, possivelmente pela falta de ação cronadora da formação reticular sobre o sistema sincronizador talâmico. 2 — Os mecanismos envolvidos na gênese dos fusos do sono barbitúrico ou espontâneo e os do cérebro isolado são, pelo menos em parte, dependentes do bloqueio da formação reticular mesencefálica. 3 — A formação reticular mesencefálica ativa preferencialmente o hemisfério cerebral homolateral; o contingente cruzado talvez seja mobilizado somente quando estímulos alertantes intensos atingem o tegmento mesencefálico. 4 — Além da formação reticular mesencefálica deve haver outros mecanismos ativadores corticais, visto que, em preparações agudas de cérebro isolado, observamos: a surtos de curta duração de atividade dessincronizada; b oscilações freqüentes do ECoG durante o registro

  2. Polymerization time for a microwave-cured acrylic resin with multiple flasks Tempo de polimerização de resina acrílica em microondas, utilizando múltiplas muflas

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    Daniela Maffei Botega

    2004-03-01

    Full Text Available This study aimed at establishing the polymerization time of a microwave-cured acrylic resin (AcronTM MC, simultaneously processing 2, 4, and 6 flasks. Required time was measured according to the parameters: monomer release in water, Knoop hardness, and porosity. Samples were made with AcronTM MC in different shapes: rectangular (32 x 10 x 2.5 mm for monomer release and porosity; and half-disc (30 mm in diameter x 4 mm in height for Knoop hardness. There were four experimental groups (n = 24 per group: G1 one flask (control; G2 two flasks; G3 four flasks, and G4 six flasks. At first, polymerization protocol was similar for all groups (3 min/450 W. Time was then adjusted for G2, G3, and G4, based on monomer release evaluation in the control group, obtained by spectrophotometer Beckman DU-70, with emitting wave of 206 nm. Knoop hardness test was performed using a Shimadzu HMV 2000 hardness tester, and 10 indentations were performed on each specimen's surface. Porosity was assessed after specimens were immersed in black ink and the pores counted in a microscope. Results showed that the complete polymerization of the resin occurred in 4.5 min for two flasks (G2; 8.5 min for four flasks (G3; and 13 min for six flasks (G4, all with 450 W. Statistical analysis revealed that the number of flasks does not interfere with polymerization, Knoop hardness, and porosity of the resin. Results showed that polymerization of microwave-curing resin with more than one flask is a viable procedure, as long as polymerization time is adjusted.O objetivo deste estudo foi o de determinar os tempos necessários para a polimerização padrão de uma resina acrílica em microondas, utilizando várias muflas simultaneamente. Os tempos necessários foram aferidos por parâmetros como monômeros liberados em água, dureza Knoop e porosidade. As amostras, confeccionadas em resina AcronTM MC, apresentavam as seguintes dimensões: para os parâmetros monômero residual e porosidade

  3. 131I对Graves病外周血粒细胞集落刺激因子和白细胞的影响%Effects of 131I treatment on the circulating granulocyte colony-stimulating factor and leucocyte levels in patients with Graves' disease

    Institute of Scientific and Technical Information of China (English)

    蔡强军; 潘芙蓉; 张琦; 李焕斌; 夏玉祥; 谢克俭

    2011-01-01

    Objective To observe the effects of 131I treatment on circulating granulocyte colonystimulating factor (G-CSF) and leucocyte levels of patients with Graves' disease (GD).Methods Enzyme-linked immunosorbent assay (ELISA),coulter three assortments,and radioimmunoassay were used to test the levels of circulating G-CSF,leucocytes and thyroid hormones of 65 incipient and untreated GD patients,all females,aged 21 -50,43 with normal leucocyte level and 22 with leucopenia before and after 131I treatment.Thirty age-matched healthy female subjects were used as controls.Results Before 131I treatment,the serous G-CSF level of the GD patients with normal leucocyte level was (28.4 ± 11.7)μg/L,significantly higher than that of the control [ ( 18.3 ± 6.98) μg/L,t =2.376,P < 0.05 ].The serous G-CSF level of the GD patients with leucopenia was (40.1 ± 13.8 ) μg/L,significantly higher than that of the patients with normal leucocyte level ( t =2.788,P < 0.01 ) and that of the control ( t =3.672,P<0.01 ).180 d after the initiation of 131 I treatment,the G-CSF level of the patients with normal leucocyte level was (18.9 ± 8.32) μg/L,not significantly different from that of the normal controls,however,the G-CSF level of the GD patients with leucopenia was (25.7 ± 11.5) μg/L,still significantly higher than that of the normal control (t =2.103,P < 0.05).The serous G-CSF level was negatively correlated with the titer of leucocyte ( r =- 0.38,P < 0.05 ),however,not significantly correlated with such clinical parameters,as free triiodothyronine (FT3),free thyroxine (FT4) and thyrotropin-stimulating hormone (TSH).Conclusions Abnormal increment of G-CSF is observed in the GD patients,which may be related to the decrease of leucocyte.Effectively suppressing the auto-immune status in the GD patients,131I treatment is a safe and reliable therapy for GD patients with leucopenia and should be used as early as possible.%目的 探讨131I治疗Graves病(GD)对患者外周血粒细

  4. Atributos químicos de um latossolo bruno sob sistema plantio direto em função da estratégia de adubação e do método de amostragem de solo Chemical properties of a haplohumox under no-tillage as related to fertilization strategies and soil sampling method

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    Volnei Pauletti

    2009-06-01

    greater in the no-tillage system because there is no homogenization of the soil surface layer. This study evaluated the effect of fertilization strategies on soil chemical properties at distinct soil depths after three and six years from the beginning of the experiment in a Haplohumox under long-term no-tillage. Besides, the variation of these properties considering two methods of soil sampling, with and without inclusion of the seeding row, was evaluated. Ten treatments were applied, nine considering P sources (rock phosphate and triple superphosphate, row preparation equipment (double disc opener and coulter opener, fertilizer placement (in-row and broadcast, time of application (summer and winter and one control treatment, without fertilizer. The measured soil chemical properties were higher in the surface layers and depth distribution, except for P, was not influenced by the fertilization strategies. The soil P values were the lowest in the 0-5 cm layer where the coulter opener equipment had been continuously used for more than three years and no fertilizer was applied. With the inclusion of the sowing row in the soil sampling, higher K, Ca and CEC values were observed. The methodologies of soil sampling, with and without the inclusion of the sowing row and the 0-10 and 0-20 cm sampling layers resulted in similar fertilizer and lime recommendations for areas under long-term no-tillage.

  5. Métodos de detección de la mastitis bovina (Methods of detection of the bovine mastitis

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    Bedolla, CC

    2007-09-01

    Full Text Available ResumenEste artículo es una revisión bibliográfica sobre los métodos de detección de la mastitis bovina que se utilizan más comúnmente en el mundo. La mastitis bovina es la inflamación de la glándula mamaria causada por una infección por patógenos. Es una de las enfermedades más frecuentes de la producción que afecta a la industria lechera en todo el mundo. Puede presentarse de manera clínica y subclínica. La mastitis subclínica es de larga duración y es mucho más frecuente que la mastitis clínica. Dentro de los métodos que se usan con mayor frecuencia a nivel de campo para diagnosticar mastitis clínicas, se encuentran el método de observación y palpación de la ubre y las pruebas físicas, como la prueba de escudilla de ordeño, prueba del paño negro y taza probadora. Las pruebas químicas, como la prueba de conductividad eléctrica de la leche, papel indicador de mastitis y prueba de whiteside que sirven también para diagnosticar mastitis clínicas y subclínicas. Las pruebas biológicas, como son la prueba de California para mastitis, la prueba de Wisconsin, el diagnóstico bacteriológico por los métodos de aislamiento, cultivo, tinción, pruebas bioquímicas e identificación y el conteo de células somáticas por microscopia directa y el somaticell. Otros métodos utilizados actualmente por su rapidez y efectividad son los electrónicos como el fossomatic y el counter coulter, los cuales tienen una aplicación universal sobre todo en laboratorios de control lechero o dedicados al diagnóstico e investigación de la mastitis y el DeLaval cell counter. Los métodos de detección de mastitis son una herramienta que permite identificar el tipo de infección clínica o subclínica que puede presentarse dentro de un hato lechero, por lo que el método que se elija para determinar las pruebas será esencial para tener un diagnóstico más preciso.AbstractThis article is a bibliographical revision on the methods of

  6. PARTICAL SIZE EVOLUTION IN SURFACTANT-FREE EMULSION COPOLYMERIZATION OF STYRENE AND METHYL METHACRYLATE IN AQUEOUS SOLUTION OF POLY ( ETHYLENE GLYCOL)%苯乙烯与甲基丙烯酸甲酯在聚乙二醇水溶液中无皂乳液共聚的粒径变化

    Institute of Scientific and Technical Information of China (English)

    施一鸣; 单国荣

    2012-01-01

    The surfactant-free emulsion polymerization ( SFEP) of styrene and methyl methacrylate carried out in the aqueous solution of poly ( ethylene glycol) (PEG) was studied. With high power optical microscope, Coulter counter and transmission electron microscopy, particle size, particle size distribution and zeta potential were measured in the process of polymerization. It is interesting to observe that the polymer particle size experiences a process from small to large,which can be more than 1000 μm,and then from large to small, which is back to a range of 0. 04 - 0. 18 μm. When the particle size returns to nanometer scale, the system has an obvious temperature rising. This phenomenon is different from the traditional nucleation mechanism of SFEP. The particle, which is formed by the homogeneous nucleation or oligomer micelle nucleation mechanism that is popularly received as traditional nucleation mechanism of SFEP, usually has a trend to become bigger and bigger to the end in the process of SFEP. It is obviously found that the freshly formed particles are first coalesced to form a particle pile,which is bigger than 1000 pun and even seen with eyes,and then corrupted into tinny ones, which are smaller than 0. 04 u,m and even beyond the ability of high power optical microscope. With transmission electron microscopy (TEM), it was clearly proven the existence of paricle pile which was composed of a lot of little particles ranging from 40 nm to 80 nm. And by use of Coulter zeta potential measurement, it was found that the zeta potential of particles had a trend from low to high level, and experenced abruptly increase in zeta potential at 60 min which was just the time that the little particle seperated from the paricle pile. This phenomenan was very different from the system that SEFP was carryed out in water without PEG, in which the zeta potential of particle was keeping about -50 mV and just changed a little after 50 min. It was reasonble to think that the potential

  7. 4LT-A型错行作业挖掘甜菜联合收获机研制与试验%Development and experiment of 4LT-A staggered-dig sugar beer combine

    Institute of Scientific and Technical Information of China (English)

    顾峰玮; 胡志超; 吴惠昌; 彭宝良; 高学梅; 王申莹

    2014-01-01

    Sugar beet was the most important source of sugar in the sugar industry, with the sugar amounting to about 17% of China's total sugar production. As a seasonal operation, sugar beet harvesting in China was still mainly manual work, having the problems of high labor intensity, low efficiency, great harvest losses and huge occupation of farming and so on, which constrained the further development of the sugar beet industry. Mechanized harvesting was the inevitable development trend of sugar beet harvest. Beet harvest mechanization development had lagged in our country for a long time. And we were lack of a thorough system research about sugar beet harvest mechanization technology, especially the advanced combine technology. The current domestic sugar beet harvesters were basically simple sectional operating machinery. Sugar beet combine harvesters were mainly imported from abroad, having high automation and intelligence degree and advanced performance. But imported machines had the problems of poor adaptability and high prices. The domestic beet combines were still in the prototype development stage, having a great gap with the advanced foreign technology. In order to improve the mechanization level and reduce the costs of sugar beet harvest machine, a staggered-dig sugar beet combine was developed, fully digesting and absorbing advanced technology, combined with the sugar beet grain planting scale, economic conditions, and the present situation of domestic machinery manufacturing level. The overall structure includes traction frame, row detection mechanism, round coulter, copying cut top institution, digging and conveying institution, side of threshing mechanism, walking system, cage type rotary lifting device, conveying equipment, main frame, stock bin, top conveyer and so on. The sugar beet combine adopted the key technologies such as top contour cutting, auto-follow row system, hydraulic technology, sensor signal acquisition technology and microprocessor control

  8. Infrared Absorption by Atmospheric Aerosols in Mexico City during MILAGRO.

    Science.gov (United States)

    Kelley, K. L.; Mangu, A.; Gaffney, J. S.; Marley, N. A.

    2007-12-01

    Past research in our group using cylindrical internal reflectance spectroscopy has indicated that aqueous aerosols could contribute to the radiative warming as greenhouse species (1,2). Although aerosol radiative effects have been known for sometime and are considered one of the major uncertainties in climate change modeling, most of the studies have focused on the forcing due to scattering and absorption of radiation in the uv- visible region (3). Infrared spectral information also allows the confirmation of key functional groups that are responsible for enhanced absorption observations from secondary organics in the uv-visible region. This work extends our efforts to evaluate the infrared absorption by aerosols, particularly organics, that are now found to be a major fraction of urban and regional aerosols in the 0.1 to 1.0 micron size range and to help identify key types of organics that can contribute to aerosol absorption. During the MILAGRO campaign, quartz filter samples were taken at 12-hour intervals from 5 am to 5 pm (day) and from 5 pm to 5 am (night) during the month of March 2006. These samples were taken at the two super-sites, T-0 (Instituto Mexicano de Petroleo in Mexico City) and T-1 (Universidad Technologica de Tecamac, State of Mexico). The samples have been characterized for total carbon content (stable isotope mass spectroscopy) and natural radionuclide tracers, as well as for their UV-visible spectroscopic properties by using integrating sphere diffuse reflectance spectroscopy (Beckman DU with a Labsphere accessory). These same samples have been characterized in the mid and near infrared spectral ranges using diffuse reflection spectroscopy (Nicolet 6700 FTIR with a Smart Collector accessory). Aerosol samples were removed from the surfaces of the aerosol filters by using Si-Carb sampler. The samples clearly indicate the presence of carbonyl organic constituents and the spectra are quite similar to those observed for humic and fulvic acids

  9. Enhanced UV Absorption in Carbonaceous Aerosols during MILAGRO and Identification of Potential Organic Contributors.

    Science.gov (United States)

    Mangu, A.; Kelley, K. L.; Marchany-Rivera, A.; Kilaparty, S.; Gunawan, G.; Gaffney, J. S.; Marley, N. A.

    2007-12-01

    Measurements of aerosol absorption were obtained as part of the MAX-Mex component of the MILAGRO field campaign at site T0 (Instituto Mexicano de Petroleo in Mexico City) during the month of March, 2006 by using a 7- channel aethalometer (Thermo-Anderson). These measurements, obtained at 370, 470, 520, 590, 660, 880, and 950 nm at a 5 minute time resolution, showed an enhanced absorption in the UV over that expected from carbon soot alone. Samples of fine atmospheric aerosols (less than 0.1micron) were also collected at site T0 and T1 (Universidad Technologica de Tecamac, State of Mexico) from 5 am to 5 pm (day) and from 5 pm to 5 am (night) during the month of March 2006. The samples were collected on quartz fiber filters with high volume impactor samplers. The samples have been characterized for total carbon content (stable isotope ratio mass spectroscopy) and natural radionuclide tracers (210Pb, 210Po, 210Bi, 7Be, 13C, 14C, 40K, 15N). Continuous absorption spectra of these aerosol samples have been obtained in the laboratory from 280 to 900nm with the use of an integrating sphere coupled to a UV-visible spectrometer (Beckman DU with a Labsphere accessory). The integrating sphere allows the detector to collect and spatially integrate the total radiant flux reflected from the sample and therefore allows for the measurement of absorption on highly reflective or diffusely scattering samples (1). The continuous spectra also show an enhanced UV absorption over that expected from carbon soot and the general profiles are quite similar to those observed for humic and fulvic acids found as colloidal materials in surface and groundwaters (2), indicating the presence of humic-like substances (HULIS) in the fine aerosols. The spectra also show evidence of narrow band absorbers below 400 nm typical of polycyclic aromatics (PAH) and nitrated aromatic compounds. Spectra were also obtained on NIST standard diesel soot (SRM 2975), NIST standard air particulate matter (SRM 8785

  10. Progress toward curing HIV infection with hematopoietic cell transplantation

    Directory of Open Access Journals (Sweden)

    Petz LD

    2015-07-01

    Full Text Available Lawrence D Petz,1 John C Burnett,2 Haitang Li,3 Shirley Li,3 Richard Tonai,1 Milena Bakalinskaya,4 Elizabeth J Shpall,5 Sue Armitage,6 Joanne Kurtzberg,7 Donna M Regan,8 Pamela Clark,9 Sergio Querol,10 Jonathan A Gutman,11 Stephen R Spellman,12 Loren Gragert,13 John J Rossi2 1StemCyte International Cord Blood Center, Baldwin Park, CA, USA; 2Department of Molecular and Cellular Biology, Irell and Manella Graduate School of Biological Sciences, 3Department of Molecular and Cellular Biology, Beckman Research Institute, City of Hope, Duarte, CA, USA; 4CCR5-Δ32/Δ32 Research Department, StemCyte International Cord Blood Center, Baldwin Park, CA, USA; 5Department of Stem Cell Transplantation, University of Texas MD Anderson Cancer Center, Houston, TX, USA; 6MD Anderson Cord Blood Bank, Department of Stem Cell Transplantation, University of Texas MD Anderson Cancer Center, Houston, TX, USA; 7Carolinas Cord Blood Bank, Duke University Medical Center, Durham, NC, USA; 8St Louis Cord Blood Bank, SSM Cardinal Glennon Children's Medical Center, St Louis, MO, USA; 9Enhance Quality Consulting Inc., Oviedo, FL, USA; 10Cell Therapy Service and Cord Blood Bank, Banc de Sang i Teixits, Barcelona, Spain; 11BMT/Hematologic Malignancies, University of Colorado, Aurora, CO, USA; 12Immunobiology and Observational Research, CIBMTR, Minneapolis, MN, USA; 13National Marrow Donor Program/Be The Match, Minneapolis, MN, USA Abstract: HIV-1 infection afflicts more than 35 million people worldwide, according to 2014 estimates from the World Health Organization. For those individuals who have access to antiretroviral therapy, these drugs can effectively suppress, but not cure, HIV-1 infection. Indeed, the only documented case for an HIV/AIDS cure was a patient with HIV-1 and acute myeloid leukemia who received allogeneic hematopoietic cell transplantation (HCT from a graft that carried the HIV-resistant CCR5-Δ32/Δ32 mutation. Other attempts to establish a cure for HIV

  11. The Argonne Leadership Computing Facility 2010 annual report.

    Energy Technology Data Exchange (ETDEWEB)

    Drugan, C. (LCF)

    2011-05-09

    that will be faster than petascale-class computers by a factor of a thousand. Pete Beckman, who served as the ALCF's Director for the past few years, has been named director of the newly created Exascale Technology and Computing Institute (ETCi). The institute will focus on developing exascale computing to extend scientific discovery and solve critical science and engineering problems. Just as Pete's leadership propelled the ALCF to great success, we know that that ETCi will benefit immensely from his expertise and experience. Without question, the future of supercomputing is certainly in good hands. I would like to thank Pete for all his effort over the past two years, during which he oversaw the establishing of ALCF2, the deployment of the Magellan project, increases in utilization, availability, and number of projects using ALCF1. He managed the rapid growth of ALCF staff and made the facility what it is today. All the staff and users are better for Pete's efforts.

  12. 2702例孕妇中期胎儿唐氏征筛查结果的分析%Screening Analysis of Down's Syndromes in 2702 cases on Middle Period Pregnancy

    Institute of Scientific and Technical Information of China (English)

    江先雨; 刘志伟; 叶飞娣

    2012-01-01

      目的分析2702例孕妇中期胎儿唐氏征筛查结果,探讨孕妇中期胎儿唐氏征产前筛查的临床意义.方法采用贝克曼库尔特全自动化学发光酶免分析仪及原装试剂,对孕14~20+6周的孕妇血清进行甲胎蛋白(AFP)、人绒毛膜促性激素β亚基(β-HCG)及游离雌三醇(uE3)检测,并将孕妇的相关信息和检测的 AFP、β-HCG、uE3结果输入“腾程筛查软件”中,进行三联检测的结果运算,得出一个概率指标.结果唐氏综合征(DS)筛查结果高危例数有65例,总阳性率为2.41%;其中:20~24岁年龄组的孕妇唐氏综合征筛查结果高危例数有11例(1.76%)25-29岁年龄组的孕妇唐氏综合征筛查结果高危例数有26例(1.93%);30-35岁年龄组的孕妇唐氏综合征筛查结果高危例数有9例(2.77%);>35岁年龄组的孕妇唐氏综合征筛查结果高危例数有19例(4.73%).结论在中期孕妇进行唐氏综合征三联筛查是必要的,能够提高唐氏患儿的早期发现和诊断,是降低唐氏患儿出生率的有效方法.%  Objective To discussed the middle for pregnant women fetal down the clinical significance of prenatal screening,2702 cases were analyzed for pregnant women fetus down the middle screening results. Methods Used the Beckman Kurt automatic chemiluminescence enzyme from analyzer and original reagent to detection of AFP, β-HCG and uE3 content in pregnant women of 14~20+6 weeks. Pregnant women's information and AFP, β-HCG and uE3 assessment input "Tengcheng software" for joint screening, get a probability index. Results Down syndrome (DS) screening high-risk number results 65 cases, the total prevalence rate was 2.41%; Among them: 20~24 years old age group of pregnant women down's syndrome screening results have high-risk number 11 cases(1.76%); 25~29 age group of pregnant women down's syndrome screening results high-risk number 26 cases (1.93%); 30~35 years old age group of pregnant women down

  13. "The human race is still a work in progress":An Interview with Richard Powers%"人类仍是一部进步中的作品":理查德·鲍尔斯访谈录

    Institute of Scientific and Technical Information of China (English)

    刁克利

    2007-01-01

    理查德·鲍尔斯是2006年美国国家图书奖小说奖得主,获奖作品《回声制造者》.他于1998年当选美国艺术与科学院院士,现任伊利诺伊大学厄巴纳-香槟分校英语系教授兼贝克曼高级研究院研究员.他屡获殊荣的另外8部小说包括:《我们歌唱的时代》、《冲破黑暗》、《赢利》、《葛拉蒂2.2》、《囚犯的困境》、《三个农民去舞会》等.这次访谈以作家的产生和小说的创作为主要内容,集中讨论了他的作家观和主要创作经历,同时也讨论了他在不同文化中的丰富经历及其对科学技术的广泛探索.在访谈中,他还就好小说给出了他的定义,阐释了作家主要关心的问题和特殊的创作视角,表达了他对文学批评的看法以及他对文学启迪人生意义的信念.本访谈是伊利诺伊大学东亚及太平洋研究中心弗里曼基金项目"创作视角与文学精神:当代美国作家研究"的部分成果.%Richard Powers is the 2006 winner of National Book Award for his fiction The Echo Maker. He was elected fellow of the American Academy of Arts and Sciences in 1998, and he is professor at the Department of English and a full-time member of the Beckman Institute, University of Illinois at Urbana-Champaign(UIUC). He is also author of eight other prize-winning novels:The Time of Our Singing, Plowing the Dark, Gain, Galatea 2.2, Operation Wandering Soul, The Gold Bug Variations, Prisoner's Dilemma and Three Farmers on Their Way to a Dance. This interview concentrates on questions about his idea of being a writer and the making of fiction in general, and also discusses his rich experience of different cultures and his extensive exploration in science and technology. Furthermore, Powers talks about the differences between writing fiction and pursuing science. He gives his definition of good fiction, and explains his main concerns as a writer, his vision of literary criticism, and his belief in

  14. Effect of anticancer therapy on Tn antigen exposure on the leucocyte membranes in patients with leukemia

    Directory of Open Access Journals (Sweden)

    G. S. Maslak

    2014-08-01

    Full Text Available Tn-antigen (Thomsen-nouvelle antigen is tumor-associated carbohydrate antigen with only one GalNAc residue attached to serine or threonine of polypeptide chain. There is not enough data about the expression of this glycotope in hematologic processes. But the correlations between increasing Tn-antigen expression on the cell surface and tumor growth progression, invasion, and activation of cell migration are well known. Therefore, the currently important area of modern research is studying of the impact of anticancer therapy by expression of this carbohydrate antigen in the onco-proliferative process. There are two types of cytostatic therapies in clinical hospitals of Ukraine: COP-therapy (cyclophosphamide, vincristine, prednisone and FC-therapy (fludarabine, cyclophosphamide, which are the most popular due to their effectiveness and low price. The aim of our study was to investigate Tn-antigen exposure on the surface of lymphocytes, monocytes and granulocytes in polycythemia vera and subleukemic myelosis; to examine the influence of COP- and FC-therapies on Tn-antigen exponation in patients with chronic lymphocytic leukemia. The objects of the study were blood cells of patients with chronic lymphocytic leukemia (n = 25, polycythemia vera (n = 15 and subleukemic myelosis (n = 15 aged 58–66 years. Healthy hematologic volunteers (n = 15 aged 55 to 65 years were in the control group. Lymphocytes of patients with chronic lymphocytic leukemia (n = 25 were also studied after the chemotherapy treatment of patients divided into two groups: those who took COP-therapy (n = 13; and those who treated with FC-therapy (n = 12. Tn-antigen exposure on lymphocytes, monocytes and granulocytes was investigated by Beckman Сoulter EPICS flow cytometer with primary monoclonal Tn-antigen anybodies (Institute of Immunology, Moscow, Russia and secondary fluorescein isothiocyanate labeled antybodies (Millipore, USA. The number of dead cells was monitored by binding

  15. 匙吻鲟的细胞遗传学分析%CYTOGENETIC ANALYSIS OF POLYODON SPATHULA

    Institute of Scientific and Technical Information of China (English)

    薛淑群; 尹洪滨

    2009-01-01

    Metaphase chromosome specimens of Polyodon spathula, injected with PHA and colchicin, were prepared from short-term culture of kidney cells with air-drying technique. It diploid chromosome number was 2n = 120. The karyotype was composed of 44m, 32sm and 44st, t chromosomes, NF = 196. Diploid nucleus DNA content was measured from the somatic cell of P. spathula, using flow cytometer( Beckman cell Lab Quant, made in USA) and the DNA content (2.3pg/ N) of erythrocytes of chicken ( Gallus domestica) as standard. The DNA content of P. spathula was 6.18pg/N , which was corresponded with the result measured chromosome number as ploidy. According to the present results and date reported, P. spathula was possibly a tetraploid (4n). Combination of the present results and data reported among Acipenseriformes including two families and six genera, Acipenseriformes was the polyploid origin's fish, and was quite completely special at the cell level which evolved by chromosome doubling. The chromosome's doubling and differentiation possibly were primary caused of polyploidization and polyploid type (4n, 8n, 12n and so on) richly. Polyoidization had played an important role in the diversity of Acipenseriformes.%采用体内注射PHA和秋水仙素,肾细胞短期培养,常规空气干燥法制备匙吻鲟(Polyodon spathula)的染色体标本.对其肾细胞染色体数目统计分析表明,匙吻鲟染色体组南120条染色体所组成.以测得的核型参数和按Levan,et al.提出的染色体划分标准得出:具有22对中部着丝粒染色体(m),16对亚中部着丝粒染色体(sm),22对亚端部着丝粒染色体(st)和端部着丝粒染色体(t);染色体臂数(NF)为196,核型公式为44 m + 32am + 44st,t.再采用流式细胞仪分析系统测定匙吻鲟体细胞的DNA含量,与鸡血细胞标准对照相比为2.69±0.19,以鸡红细胞DNA含量2.3 pg/N测算.则匙吻鲟体细胞DNA含量为6.18 pg/N.根据所得到的结果并结合已发表的鲟鱼类DNA含量和

  16. 微量快速测定木糖法检测创伤后肠道吸收功能的实验研究%Experimental study of gut absorptive capacity after trauma detected by D-xylose content test with micromethod

    Institute of Scientific and Technical Information of China (English)

    黎君友; 孙丹

    2003-01-01

    目的寻找简单、快捷、精确、稳定和微量的检测肠道吸收功能的方法,以作判断严重创伤后肠道吸收功能的诊断指标.方法以Eberrs等建立的方法作适当调整,使之微量和动态测定小动物血和尿中木糖的含量.反复作5次标准曲线,测定Wistar大鼠空腹血和6只口服0.5g/kg木糖(D-xylose)后大鼠2和4 h血的含量,并测定7只肠缺血再灌注大鼠血木糖含量.结果经DU-7 Backman扫描证实最大吸收峰在554 nm,以5次0~4 mmol/L范围内测定木糖标准曲线呈良好的线性关系(r=0.9979±0.0017);大鼠口服木糖后2 h血含量(154±6)mg/L;4 h后降至(87±11)mg/L.缺血再灌注后2和4 h血含量分别为162±5和(80±8)mg/L,较正常大鼠2 h吸收快.取大鼠口服2和4 h血浆批内重复6次,批间重复6次,结果批内变异系数为1.98%,批间变异系数为3.10%;各标准木糖回收率为97.2%~104.3%.结论微量快速测定木糖方法简单、快捷、稳定,可用作判断严重创伤后肠道吸收功能损伤的指标.%Aim To establish a rapid and sensitive diagnostic markerfor adjusting gut absorptive capacity after trauma. Methods A mi-cromethod for D-xylose test with phloroglucinal was established according toEberts with modification. The standard curves were repeated for fivetimes. Then the blood contents of fasting and orally taking 0. 5 g/kgD-xylose at 2nd and 4th hour were measured and the blood D-xylose con-tents of seven refilling rats with ischemia of intestine were mea-sured. Results The maximal absorption spectra was read at 554 nm byscanming of DU-7 Beckman. Good linearity of the D-xylose standard curvewas found with the range of 0 to 4 mmol/L(r=0. 9979 ±0. 0017) . Theblood content of rats at 2nd hour after orally taking D-xylose was ( 154 ± 6)mg/L amd that at 4th hour was decreased to (87 ± 11) mg/L. And theblood content at 2nd and 4th day after ischemic refilling was (162 ± 5)mg/L and (80 ± 8) mg/L respectively. The variation coefficients

  17. Association of single nucleotide polymorphism at position 45 in adiponectin gene with plasma adiponectin level and insulin resistance in obesity

    International Nuclear Information System (INIS)

    Objective: To explore the association of single nucleotide polymorphism at position 45 (SNP45) in adiponectin gene with plasma adiponectin level and insulin resistance in obesity in Quanzhou area of Fujian province. Methods: Two hundred and forty-eight patients with obesity and 225 normal control subjects were enrolled in this study.Fasting insulin (FINS) were measured by radioimmunoassay and fasting plasma glucose (FPG), total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C) were measured by BECKMAN DXC800 biochemistry analyzer. Body mass index (BMI), waist to hip ratio,homeostasis model assessment of insulin resistance (HOMA-IR) were calculated. Plasma adiponectin levels were examined by means of enzyme-linked immunosorbentassy. The adiponectin gene SNP45 was identified by PCR-restriction fragment length polymorphism. Results: (1) Frequencies of GG+GT genotype in obesity group and normal control group were 61% and 44% respectively (χ2=14.182, P<0.01), and G allele frequencies were 35% and 25% (χ2=10.708, P<0.01). (2) In obesity group,the subjects with SNP45 GG+GT genotype had higher TG and LDL-C levels than those with TT genotype (t=2.604, P<0.01; t=5.507, P<0.01), and had lower adiponectin level than those with TT genotype (t=2.275, P<0.05), and had significantly lower HDL-L level than those with TT genotype (t=10.100, P< 0.01). (3) In normal control group,the subjects with SNP45 GG +GT genotype had significantly lower adiponectin,TG,TC levels than those with TT genotype (t=2.510, P<0.05; t=2.922, P<0.01; t=3.272, P< 0.01). (4) Logistic analysis proved that the SNP45 GG+GT genotype in obesity group was associated with decreased risk of plasma adiponectin level (OR=0.810, 95% CI : 0.673-0.975, P<0.05), and with increased risk of HOMA-IR (OR=1.746, 95% CI : 1.060-2.875, P<0.05). The SNP45 GG+GT genotype in normal control group was associated with increased risk of HOMA-IR (OR=3.962, 95

  18. Effect of propofol on glutamate-induced activation and elated inflammatory cytokines of astrocytes from spinal cord dorsal horn

    Institute of Scientific and Technical Information of China (English)

    Chengming Qin; Qing Li; Juying Liu; Tao Zhu; Yong Xiang

    2008-01-01

    BACKGROUND: Astrocytes participate in central nervous system-mediated physiological or pathological processes, such as pain. Activated dorsal horn astrocytes from the spinal cord produce nerve active substances and proinflammatory cytokines, such as interleukin-I beta (IL-1 β ), IL-6, and tumor necrosis factor-a (TNF-a ), which play important roles in pain transduction and regulation. OBJECTIVE: To investigate the effects of different doses of propofol on activation of cultured spinal cord dorsal horn astrocytes induced by glutamate, as well as changes in IL-1 β, IL-6, and TNF-a, and IL-10 (anti-inflammatory cytokine) expression in rats, and to explore the dose relationship of propofnl. DESIGN, TIME AND SETTING: The cellular and molecular biology experiment was performed at the Central Laboratory of Yunyang Medical College between March 2006 and December 2007. MATERIALS: Forty healthy, Wistar rats, aged 2-3 days, were selected. Propofol was provided by Zeneca, UK; glutamate by Sigma, USA; EPICS XL flow cytometry by Beckman culture, USA; rabbit-anti-mouse glial fibrillary acidic protein (GFAP) antibody kit and inflammatory cytokine detection kit were provided by Zhongshan Biotechnology Company Ltd., Beijing; multimedia color pathologic image analysis system was a product of Nikon, Japan. METHODS: Astrocytes were harvested from T11-L6spinal cord dorsal horn of Wistar rats and incubated for 3 weeks. The cells were divided into seven groups, according to various treatment conditions: control group was cells cultured in Hank's buffered saline solution; intralipid group was cells cultured in intralipid (0.2 mL/L); glutamate group was cells cultured with 100 μ mol/L glutamate; propofol group was cells cultured with 250 μ mol/L propofol; three glutamate plus propofol groups were cultured in 100 μ mol/L of glutamate, followed by 5, 25, and 250 μ mol/L of prnpofol 10 minutes later. MAIN OUTCOME MEASURES: GFAP-labeled astrocytes were analyzed using a multimedia

  19. Leukocyte differential count in peripheral blood "with" single tube/ten colors"flow cytometry%单管10色流式细胞术分类计数外周血白细胞

    Institute of Scientific and Technical Information of China (English)

    刘雪凯; 潘娜; 王建中; 邢莹; 屈晨雪; 普程伟; 伍平; 王卉; 刘抗援; 马兰

    2011-01-01

    目的 应用"单管10色"流式细胞术分类计数外周血白细胞,并探讨其临床应用价值.方法 通过多种单克隆抗体的选择和多重逻辑设门的研究,确定了10色流式细胞术与12种单克隆抗体组合的单管免洗外周血白细胞分类方法(简称"单管10色"方法).选取142份外周血标本,以血涂片显微镜法分类技术为参考方法,同时用Beckman Cotdter LH750全自动血细胞分析仪和"单管10色"方法分类计数白细胞,计算"单管10色"方法的临床诊断效率,并统计分析两种方法与显微镜法分类计数白细胞的相关性.以BD FACS Calibur流式细胞仪免疫分型结果为标准,计算"单管10色"流式细胞术检出原始细胞的诊断效率.结果 "单管10色"流式细胞术与显微镜法分类计数外周血白细胞除嗜碱粒细胞外均具有良好的相关性(r均>0.700,P均0.700,P<0.01) except for basophils.The correlations with neutrophilic granulocytes,lymphocytes,immature granulocytes and blasts were superior(r=0.972,0.951,0.801,0.912,respectively,P<0.01).When 1% was selected as the cut-off point for immature granulocytes determined by standard microscopic cytology,the sensitivity and the specificity of"single tube/10 colors"flow cytometry were 92%(57/62) and 79% (63/80),respectively.When 0.5% was selected as the cut-off point for blasts detected by standard microscopic cytology,the sensitivity and the specificity were 99% (67/68) and 92% (68/74).Using the immunophenotyping results from BD FACS Calibur as a standard,the sensitivity for detecting blasts by"single tube/10 colOrs"flow cytometry was 100% (40/40),the specificity was 91% (10/11),the positive predictive value was 98% (40/41),the negative predictive value was 100% (10/10) and the accuracy was 98% (50/51).Conclusions The"single tube/10 colors"flow cytometry has a excellent correlation with the standard microscopic cytology when applied on leukocyte differential count in peripheral blood.It may potentially

  20. Atividade elétrica cerebral do rato com lesões da formação reticular mesencefálica

    Directory of Open Access Journals (Sweden)

    Walter C. Pereira

    1970-09-01

    Full Text Available No presente estudo foram utilizados 73 ratos em preparações agudas e crônicas, nas quais lesamos a formação reticular mesencefálica com corrente contínua (3,5 a 4,0 mA durante 5 a 10 segundos. O eletródio ativo era implantado estereotàxicamente segundo as coordenadas de König e Klippel. As lesões eram feitas parcial ou totalmente, uni ou bilateralmente, e em todos os animais procedeu-se ao controle histológico das áreas lesadas, usando-se o método de Weil. O registro da atividade elétrica cortical foi feito com polígrafo Beckman de 4 canais, utilizando-se derivações bipolares curtas (1mm com eletródios esféricos de platina. As experiências permitiram as seguintes conclusões: 1 — As características eletrofisiológicas dos fusos que ocorrem após lesões da formação reticular mesencefálica são muito semelhantes às dos fusos espontâneos e barbitúricos, inclusive quanto à projeção cortical. Quanto à duração dos potenciais que os constituem, contudo, notamos que a faixa de variação era mais centuada (20 a 80 ms, o que pode ser atribuído à maior complexidade dos potenciais do cérebro isolado, possivelmente pela falta de ação cronadora da formação reticular sobre o sistema sincronizador talâmico. 2 — Os mecanismos envolvidos na gênese dos fusos do sono barbitúrico ou espontâneo e os do cérebro isolado são, pelo menos em parte, dependentes do bloqueio da formação reticular mesencefálica. 3 — A formação reticular mesencefálica ativa preferencialmente o hemisfério cerebral homolateral; o contingente cruzado talvez seja mobilizado somente quando estímulos alertantes intensos atingem o tegmento mesencefálico. 4 — Além da formação reticular mesencefálica deve haver outros mecanismos ativadores corticais, visto que, em preparações agudas de cérebro isolado, observamos: a surtos de curta duração de atividade dessincronizada; b oscilações freqüentes do ECoG durante o registro

  1. Expression of nerve growth factor mRNA in splenic lymphocytes of bronchial asthma rats and its influencing actors

    Institute of Scientific and Technical Information of China (English)

    Jihong Dai; Yonghong Wang; Haixia He

    2008-01-01

    BACKGROUND: Previous research has proved that nerve growth factor (NGF) participates in the onset of asthma by the induction of neurogenic inflammation.OBJECTIVE: To investigate the effect of interleukin-13 (IL-13) and interferon-γ(IFN-γ) on the expression of NGF mRNA in the splenic lymphocytes of bronchial asthma rats.DESIGN, TIME AND SETTING: The experiment, a completely randomized study based on cellular immunology, was performed in the Laboratory of Neurology in Chongqing Medical University and the Department of Clinical Pharmacy in College of Clinical Medicine, Chongqing Medical University (Chongqing, China) from January 2006 to April 2007.MATERIALS: Four adult male Wistar rats were used in this study. Rat IL-13, IFN-γprobe and the total RNA extraction kit were produced by Shanghai Sangon Biological Technology & Services Co., Ltd (China). The NGF ELISA kit was a product of Wuhan Boster Bioengineering Co., Ltd (China). A Du-70 automatic UV spectrophotometer was produced by Beckman Company (USA).METHODS: Rats were subjected to 1-mL intraperitoneal injections each containing 100 mg of ovalbumin, and were sensitized by using antigen solution, which was sensitized with 5×109 Bacillus pertussis and 100 mg aluminum hydroxide powder. Four rats were challenged with 1% ovalbumin using an ultrasonic nebulizer for 60 minutes to establish an asthmatic model. After rats were anesthetized, splenic lymphocytes were isolated and cultured in medium, which was supplemented with IL-13 or IFN-γ, for 0, 12, 24 or 48 hours. A parallel study was conducted with cultured splenic lymphocytes, which were divided into a control group, an IL-13 group and an IFN-γ group. Culture medium was added with different concentrations of IL-13 (10, 50, 100 μg/L) and IFN-γ (1, 10, 50 μg/L); 24 hours later, all samples were harvested.MAIN OUTCOME MEASURES: The expression levels of NGF mRNA were detected by reverse transcription-polymerase chain reaction.RESULTS: In the control group, the

  2. 焦虑对体液免疫功能影响及其与HLA-DQB1等位基因的关系%Influences of anxiety on humoral immune functions and the correlations with HLA-DQB1

    Institute of Scientific and Technical Information of China (English)

    高昆; 刘辉

    2009-01-01

    目的:研究焦虑对体液免疫功能的影响及其与HLA-DQB1等位基因多态性的关系.方法:随机挑选某医院健康体检住院医生31名,选用状态-特质焦虑量表(STAI)来测量其焦虑状况,实验室检测IgG、IgA、IgM以及补体C3、C4水平,并利用PCR扩增各研究对象HLA-DQB1*02、*03、*04、*05和*06五个位点基因多态性.结果:状态焦虑 (Ta)和特质焦虑 (Tc)均同补体C3呈正相关,有统计学意义.HLA-DQB1*02位点阳性和阴性的个体状态焦虑的差异有统计学意义(P<0.05),而补体C3的差异不具有统计学意义(P>0.05).HLA-DQB1* 04位点阳性和阴性的个体状态焦虑和特质焦虑的差异都有统计学意义(P<0.05),而补体C3的差异不具有统计学意义(P<0.05).结论:焦虑可以引起某些体液免疫功能指标的改变并与HLA-DQB1等位基因表型有关.%Objective:To investigate the association between anxiety and the change of humoral immune functions and its correlation with HLA-DQB1 polymorphisms.Methods:Total 31 resident doctors were selected randomly and tested by State-Trait Anxiety Inventory(STAI).IgG,IgA,IgM,complement C3 and complement C4 were detected with BECKMAN array360 system;HLA-DQB1*02、*03、*04、*05 and*06 alleles were individually amplified by polymerase chain reaction(PCR)using exon2 group-specific primers.The correlation between immune function and HLA-DQB1 polgmorphisms were investigated.Results:Statistical analysis showed that there was positive correlation with State Anxiety (Ta) and complement C3,either Trait Anxiety (Tc) and complement C3.There was significant difference between HLA-DQB1*02 positive and negative in Ta (P<0.05),while no difference in complement C3(P>0.05).There was significant difference between HLA-DQB1*04 positive and negative in Ta and Tc(P<0.05),while no difference in complement C3(P>0.05).Conclusion:Anxiety could change some humoral immune functions and this is related with HLA-DQB1 polymorphism.

  3. Radioactivity measurement with a plastic scintillation vial

    International Nuclear Information System (INIS)

    caused by CO2. One of the methods without scintillation cocktail is using a solid scintillator (ReadyCap, Beckman). However, the method needs to dry up the sample, so it requires certain time to dry up and is not applicable for volatile compounds. Also the sample could not be recovered and the solid waste would be produced. Another method is the Cherenkov counting. The method is applicable for the nuclides emitting beta rays with energy more than 260 keV, if water is used for the medium of Cherenkov effect. To detect lower energies, Takiue et al. proposed a method using a high-refractive transparent ceramic. The method, however, needs to dry up the sample. In contrast, using the ScintiVial, one does not need to dry up the sample. More than ten times use of the ScintiVial may be less expensive than the whole c.ost of liquid scintillation counting, which includes 10-mL cocktail, counting vial, and the cost for incineration. Conclusion: The ScintiVial method to measure radioactivity using a liquid scintillation counter was developed. The counting efficiency for 32P was 28-10% and that for 125I was 3%. This method is quite simple, is less expensive, and do not produce any radioactive organic liquid waste. Furthermore the sample can be recovered.

  4. H-ZSM-5催化环己酮肟制ε-己内酰胺反应机理的O NIO M研究%Reaction Mechanism of Cyclohexanone Oxime to ε-Caprolactam Catalyzed over H-ZSM-5:An ONIOM Study

    Institute of Scientific and Technical Information of China (English)

    王寒露; 周建敏; 周如金

    2014-01-01

    The Backman rearrangement reaction mechanism of cyclohexanone oxime to ε-caprolactam over the H-ZSM-5 zeolite was investigated by the ONIOM and DFT methods.All geometry structures optimization were em-ployed ONIOM (B3LYP/6-31+G(d):PM3)method.Based on the optimal configuration,a high single point cal-culation of complete 46T cluster model were carried out by a variety of density functionals,such as B3LYP/6-31+G(d),PBE/6-31+G(d),M062X/6-31+G(d)andωB97XD/6-31+G(d).Additional dispersion corrections were calculated by dftd3 program to describe the weak interaction in zeolites for B3LYP,PBE and M062X functionals. The calculation results showed that the precision of the DFT functionals for dispersion correction was greatly im-proved,which were comparable with the MP2 method.The rate determining step was the first step 1 ,2-H shift and rearrangement step,and internal reaction energy barrier was 44.5 kcal/mol.The reaction rate constants indicated that the Beckman rearrangement reaction was very slowly on the H-ZSM-5 at 293-393 K.When the temperature reached 423 K,the forward and reverse reaction rate were about the same,and the reaction occurred.When reached 623 K,the reaction rate constant was 130 1/s.%采用ONIOM和DFT方法研究了H-ZSM-5分子筛催化环己酮肟制ε-己内酰胺的贝克曼重排反应机理.所有的构型优化使用ONIOM(B3LYP/6-31+G(d):PM3)方法进行,并在此基础上对得到的最优构型应用多种密度泛度方法,如B3LYP/6-31+G(d),PBE/6-31+G(d),M062X/6-31+G(d)和ωB97XD/6-31+G(d)进行完整46T簇模型的单点能计算.B3LYP,PBE和M062X泛函使用dftd3程序计算了额外色散校正,以考虑分子筛内部的弱相互作用.计算结果显示,经色散校正的DFT方法的计算精度被大大提高,达到一个与MP2方法相媲美的精度.反应的决速步是由第一步1,2-H转移和重排步共同决定,内部反应能垒为44.5 kcal/mol.

  5. Got Varves?: Reconstructing Holocene Climate Change in Seneca Lake, NY

    Science.gov (United States)

    Rogers, C. E.; Curtin, T. M.

    2004-12-01

    The sedimentary deposits of Seneca Lake, one of eleven Finger Lakes in New York State, contain a valuable record of post-glacial climate and environmental change. Paleoenvironmental interpretations depend on knowing what transport and depositional processes controlled the formation of the laminae. In this study, we examine Holocene rhythmites in two profundal cores collected from the northern half of Seneca Lake to determine possible mechanisms of formation of the alternating olive gray-black layers. Magnetic susceptibility was measured at a 2 cm interval prior to splitting the ˜5 m cores. Split cores were described, photographed, and sampled for loss-on-ignition and grain size analysis at a 10 cm interval. When possible, core one was sampled on a lamination-by-lamination basis whereas core five was sampled at a one cm interval. Sequential LOI was used to estimate the total organic and carbonate content of sediments. Grain size analyses were performed using a Coulter LS 230 laser diffractometer after removal of calcium carbonate. Temporal control of paleoenvironmental changes in cores will be established by two accelerator mass spectrometer radiocarbon dates. The cores contain proglacial pink clay overlain by Holocene mm- to cm-scale alternating olive gray and black laminations of fine-grained sand and mud. Magnetic susceptibility changes are distinct, ranging from 1-22 x 10-6 SI units in core one and 4-57 x 10-6 SI units in core five. Sediment is dominantly composed of siliciclastic material (50-99 weight %) with varying amounts of calcite (0.1-75 weight %) plus lesser amounts of organic matter (0.5-4.1 weight %). A distinct variation in sediment color, organic matter, and carbonate content, and median grain size (φ 50) occurs in the laminated sediment. The olive gray layers are characterized by well-sorted very fine silt and clay with a φ 50 25%), and low organic matter content (1%), lower in carbonate content (6 μ m. Samples are well-sorted and positively

  6. 外周血细胞计数对肝纤维化程度的预测价值%Practical Value of Peripheral Blood Cells Count for Predicting Liver Fibrosis

    Institute of Scientific and Technical Information of China (English)

    张占卿; 陆伟; 童海涛

    2006-01-01

    目的 探讨外周血细胞计数对肝纤维化程度的预测价值.方法 外周血细胞计数采用Coulter Ac·T diff血细胞分析仪检测.用ROC曲线下面积评价外周血细胞计数对S1与S2-4,S1-2与S3-4、S1-3与S4的鉴别价值;用Bayes逐步判别分析,构建判别函数,评价外周血细胞计数对S1、S2、S3、S4的诊断价值.结果 白细胞、血小板、粒细胞计数鉴别S1-2与S3-4的ROC曲线下面积分别为0.339、0.296、0.332,显著小于诊断参考线下面积(P<0.05);红细胞、淋巴细胞、中间细胞计数鉴别S1-2与S3-4的ROC曲线下面积分别为0.414、0.436、0.502,相似于诊断参考线下面积(P>0.05).白细胞、红细胞、血小板、粒细胞、淋巴细胞、中间细胞计数鉴别S1与S2-4和S1-3与S4的ROC曲线下面积分别为0.430和0.546、0.429和0.392、0.547和0.392、0.504和0.437、0.388和0.669、0.415和0.563,均相似于诊断参考线下面积(P>0.05).根据判别函数模型,预测与实测病理分期S1、S2、S3和S4的一致率分别为50.0%、9.5%、61.8%和25.0%,总一致率为43.0%.结论 白细胞、血小板、粒细胞计数鉴别S1-2与S3-4有一定价值;白细胞、红细胞、血小板、粒细胞、淋巴细胞、中间细胞计数鉴别S1与S2-4和S1-3与S4均无价值.判别函数模型鉴别不同病理分期的价值有限.

  7. Parâmetros solo-máquina em função de doses de resíduos vegetais e profundidades de deposição de adubo em semeadura direta Parameters of soil-machine in function of the amount of cover residues and different fertilizer deposition depths in direct drilling

    Directory of Open Access Journals (Sweden)

    Karina M. Kamimura

    2009-09-01

    Full Text Available O tráfego contínuo e inadequado de máquinas em solos sob semeadura direta tem provocado alterações dos atributos físicos e mecânicos dos solos, influenciando, dessa forma, na produtividade das culturas. O experimento foi conduzido na Estação Experimental Agronômica da Universidade Federal do Rio Grande do Sul, no município de Eldorado do Sul - RS, num Argissolo Vermelho distrófico típico, com o objetivo de quantificar a força de tração e obter informações sobre os atributos físicos do solo, em semeadura direta sobre resíduos de aveia-preta e ervilhaca parcialmente decomposta. O delineamento experimental utilizado foi em blocos casualizados, com parcelas subsubdivididas, sendo, nas parcelas principais, doses de resíduos da cultura de inverno (0; 1,3; 2,6; 3,2; 3,8 e 5,1 Mg ha-1 de palha de aveia-preta consorciada com ervilhaca, nas subparcelas profundidades de atuação da haste sulcadora de adubo (0,06 e 0,12 m e nas subsubparcelas tráfegos dos rodados do trator e colhedora. Os atributos físicos do solo foram afetados pelo tráfego dos rodados da máquina. A força de tração foi influenciada pela profundidade de atuação das hastes sulcadoras e o tráfego dos rodados.The continuous and inadequate machine traffic on soils under direct drilling has been changing the soil physical and mechanical properties, influencing therefore crop productivity. The experiment was carried out at the Agricultural Research Station of the Universidade Federal do Rio Grande do Sul (Eldorado do Sul, RS, Brazil on Typic Paleudlt. The main aim of the work was to measure coulter draft requirements and physical attributes under black oats (Avena strigosa Schieb and common vetch (CV (Vicia sativa L. partially decomposed. The experimental design was carried out in randomized blocks, with split-split-plots and three repetitions. The main treatments were plots with different amount of residues of the winter crop (0, 1,3; 2,6; 3,2; 3,8 and 5,1 Mg ha-1

  8. Essai de classement typo-technologique des araires à partir des pièces métalliques découvertes en Gaule romaine en vue de leur reconstitution Setting up of a typological-technological classification method to try to reconstruct ards from metallic pieces found in Roman Gaul

    Directory of Open Access Journals (Sweden)

    André Marbach

    2008-04-01

    positions? A method of research has been developed from a fine analysis of the metallic pieces listed in a catalogue of these pieces for Gaul. The shares have been classified according to their working surface, the form of their sockets and the angle of the wearing surface with the ground if it is observable. As for the tanged shares the filing is similar, the length of the tang has however been taken into account here. From the values known for these pieces and a technical study with modelisation of the tilling implement, the possibility of a reconstruction of the ard has been explored. Researchers are here reminded of how important it is to publish the description of the pieces found with the utmost precision for the drawing as well as for the dimensions. As far as ards fitted with a socket share are concerned uncertainty prevails except for shares with a small working surface which, generally, are single ards (manche-sep. The reconstruction of tanged share-ards seems possible including the length of the draught-beam (chambige. Several concrete examples of reconstructed ards have been studied. Thus, if the reconstruction of a tanged share from metallic pieces, tang and coulter may be envisaged, that of iron share ards is uncertain except for plough-ards (reversible.

  9. 高温烟气颗粒物在线检测装置性能评价%Performance assessment of high temperature flue gas on-line particle size analyzer

    Institute of Scientific and Technical Information of China (English)

    许乔奇; 姬忠礼; 杨亮

    2012-01-01

    In order to evaluate the performance of on-line particles test equipment at high temperature, an optical on-line particle size analyzer was used to test catalyst particles concentration and particle size distribution (PSD) downstream of a fluid catalytic cracking (FCC) flue gas filtration test facility. At the same time, an off-line sampling system tested catalyst particles concentration both upstream and downstream of the facility. Catalyst PSD and microstructure were measured with Coulter counter particle size analyzer and scanning electron microscopy (SEM). The off-line test results agreed well with the results of on-line particle size analyzer. Experimental results showed that the on-line test equipment had high accuracy when operating pressure was 0. 21 Mpa and operating temperature ranged from 320°C to 465°C. Experimental and simulation results showed that operating temperature almost had no influence on test measurement. On this basis, aiming at real condition of coal chemical industry, an iterated method was developed to correct on-line measurement results, which could provide a guideline to determine the particulate matter in high temperature pipeline in coal chemical industry.%为评价高温工况下颗粒物在线检测装置的性能,在催化裂化高温烟气过滤实验装置上使用光学在线颗粒物检测装置测定了过滤器下游烟气中的催化剂浓度及粒径分布,同时用离线式粉尘等动采样装置和Coulter粒径分析仪测最过滤器上游与下游的催化剂浓度和粒径分布,对在线式的测量结果进行验证.考虑了温度对在线式测量结果的影响.实验结果表明:在线式检测装置可以在操作温度320~465℃,操作压力0.21 MPa工况下实现稳定的测最,在线式测量结果和离线式测量结果吻合很好,实验和计算模拟结果表明操作温度对实验测量的影响可以忽略.在此基础上,针对煤化工行业的实际工况,提出了利用迭代方式来获得

  10. The influence of melanin on bystander effect

    International Nuclear Information System (INIS)

    The influence of melanin on bystander effect has been studied. Melanin is known to be a natural substance with proved radioprotective properties. It is effective only against low dose radiation. The observed result in colony-forming activity of HPV-G cells may be due to the potential radioprotective effect of melanin. HPV-G cells (human keratinocyte line, immortalised by transfection with the HVP virus) are cultured in Dulbecco's MEM: F12 (1:1) Medium. In addition, 50 ml of Fetal Calf Serum, 5 ml penicillin-streptomycin, 5 ml L-glutamine and 1 g/ml of hydrocortisone were added to medium. Cells were maintained in an incubator at 37 degrees Celsius, with 95% humidity and 5% Carbon Dioxide and routinely subcultured every 8-10 days. Melanin was isolated from animal hair (Belarus Pharmaceutical Association, Minsk). Melanin was added to cell medium at 1 mg/l concentration. Cell suspension after dilution was counted using a Coulter counter. Appropriate cell numbers were plated according to the Puck and Marcus technique in 5 ml medium in 25cm2 NUNC flasks. There were 3 types of flasks: direct irradiation, bystander donor and bystander recipient. Each of 3 them had 4 triplet sets: control, melanin, irradiated cells 0,5 Gy and irradiated cells 0,5 Gy with melanin added. Melanin is added into flasks 30 min - 1 hour before irradiation. Bystander donor and direct flasks are irradiated and replaced back in the incubator at 37 degree centigrade for one hour. Culture flasks are irradiated at room temperature using a Co 60 teletherapy source, delivering approximately 2 Gy per minute at a source-to cell distance of 80 cm. 1 hour after irradiation the medium from bystander donor flasks is removed and filtered through NALGENE 0,2 m sterile syringe filter to ensure that no cells are present in the medium. This filtered medium is used to replace the medium from bystander recipient flasks. All flasks are left for 9-10 days (until colonies are visible) and then stained with Carbol Fusion

  11. 长期低剂量微波辐射对大鼠血清生化和心脏结构的影响研究%Effects of long-term low doses of microwave radiation on biochemical indexes of serum and the myocardial histological structure in rats

    Institute of Scientific and Technical Information of China (English)

    张静; 彭瑞云; 王水明; 高亚兵; 宋薇; 李翔; 董霁; 赵黎

    2011-01-01

    目的 探讨长期低剂量微波辐射致大鼠血清生化、心脏功能及结构的影响.方法 采用2.5、5和10 mW/cm2 微波辐射160只二级雄性Wistar大鼠,5次/周×30 d,6 min/次.于辐射后1 d、14 d、60 d和180 d,采用全自动生化检测仪检测血清钾离子(K+)、钠离子(Na+)、天冬氨酸转氨酶(aspartate aminotransferase,AST)和谷氨酸转氨酶(alanine aminotransferase,ALT)含量;于辐射后6 h、7 d、14 d、30 d和60 d活杀大鼠取心脏,采用光镜和透射电镜观察心脏组织结构及超微结构的改变.结果 2.5、5和10 mW/cm2组大鼠血清Na+含量分别于照射后1 d(P<0.05)和14 d(P<0.01)降低,60 d(P<0.01)和180 d(P<0.05)表达持续升高;AST和ALT含量分别于照射后14 d显著降低(P<0.05);180 d见恢复.2.5、5和10 mW/cm2 微波辐射后7~30 d,大鼠心肌纤维排列紊乱,线粒体肿胀、空化,闰盘结构模糊,此病变随辐射剂量增加呈加重趋势.结论 2.5~10 mW/cm2微波长期辐射可致大鼠K+、Na+、AST及ALT含量异常及心脏组织结构与超微结构损伤.%Objective To investigate the changes of the serum biochemical index and myocardial histological structure in rats after long-term low doses of microwave exposure. Methods One hundred and sixty male Wistar rats were continuously exposed to microwave of 2. 5, 5 and 10 mW/cm2 in a month, 5 times a week and 6 minutes each time. The left ventricle blood was obtained at 1 d, 14 d and 60 d after the rats were irradiated. The K+ . Na+ , AST and ALT level was analysed by Coulter automatic biochemical analyzer. All the experimental rats were sacrificed at 6 h, 7 d, 14 d, 30 d and 60 d after exposure, then the histology and ultrastructure of the heart were observed by light and electron microscope. Results After 2. 5 , 5 and 10 mW/cm2 microwave exposure, heart muscle fibers wavilness , mitochondrial swelling and cavitations,blurred intercalary dics were observed at 7 d - 30 d. Conclusions A long-term of 2. 5 - 10 m

  12. The role of versican G3 domain in regulating breast cancer cell motility including effects on osteoblast cell growth and differentiation in vitro – evaluation towards understanding breast cancer cell bone metastasis

    Directory of Open Access Journals (Sweden)

    Du William

    2012-08-01

    Full Text Available Abstract Background Versican is detected in the interstitial tissues at the invasive margins of breast carcinoma, is predictive of relapse, and negatively impacts overall survival rates. The versican G3 domain is important in breast cancer cell growth, migration and bone metastasis. However, mechanistic studies evaluating versican G3 enhanced breast cancer bone metastasis are limited. Methods A versican G3 construct was exogenously expressed in the 66c14 and the MC3T3-E1 cell line. Cells were observed through light microscopy and viability analyzed by Coulter Counter or determined with colorimetric proliferation assays. The Annexin V-FITC apoptosis detection kit was used to detect apoptotic activity. Modified Chemotactic Boyden chamber migration invasion assays were applied to observe tumor migration and invasion to bone stromal cells and MC3T3-E1 cells. Alkaline phosphatase (ALP staining and ALP ELISA assays were performed to observe ALP activity in MC3T3-E1 cells. Results In the four mouse breast cancer cell lines 67NR, 66c14, 4T07, and 4T1, 4T1 cells expressed higher levels of versican, and showed higher migration and invasion ability to MC3T3-E1 cells and primary bone stromal cells. 4T1 conditioned medium (CM inhibited MC3T3-E1 cell growth, and even lead to apoptosis. Only 4T1 CM prevented MC3T3-E1 cell differentiation, noted by inhibition of alkaline phosphatase (ALP activity. We exogenously expressed a versican G3 construct in a cell line that expresses low versican levels (66c14, and observed that the G3-expressing 66c14 cells showed enhanced cell migration and invasion to bone stromal and MC3T3-E1 cells. This observation was prevented by selective EGFR inhibitor AG1478, selective MEK inhibitor PD 98059, and selective AKT inhibitor Triciribine, but not by selective JNK inhibitor SP 600125. Versican G3 enhanced breast cancer cell invasion to bone stromal cells or osteoblast cells appears to occur through enhancing EGFR/ERK or AKT signaling

  13. 生长激素对胰腺癌IGF-Ⅰ,Ⅱ-IGFBP3通路动态影响的实验研究%Effect of growth hormone on features of IGF-Ⅰ-Ⅱ-IGFBP3 pathway in pancreatic cancer

    Institute of Scientific and Technical Information of China (English)

    石毅; 孙跃明; 白剑锋; 陆文熊; 傅赞; 赵翰林; 苗毅

    2010-01-01

    Objective To investigate the effect of GH on proliferation of pancreatic cancer cells and observe the features of IGF-IGFBP3 pathway in the host after GH administration. Methods Pancreatic cancer cells (SW-1990,PANC-1 and P3) during exponential growth stage were harvested and cultured in medium containing growth hormone (50 ng/ml). After 24, 48 and 72 hours, cells were counted using a Coulter Counter. Thirty-five Athymic nude Balb/c mice were inoculated with SW-1990cells. When tumors became palpable after inoculation, animals were randomized to receive GH points (1 h, 2 h, 6 h, 24 after the last injection), plasma samples were gathered for subsequent ELISA determination and liver was rapidly incised for immune blotting analysis. Results The results revealed that GH stimulated cell growth in vitro. GH elevated levels of IGF-Ⅰ , Ⅱ at the 1st , 2nd , 6th hour after the last injection. GH augmented the expression of IGFBP3 in the liver of the host in vivo (1 h, 2 h, 6 h, 24 h, respectively). Conclusion Such proteins as IGF- Ⅰ and Ⅱ might be associated with mechanism of last effect of GH on tumor host. The up-regulation of IGFBP3 by GH administration in the host may help to explain the phenomena that GH doesn't accelerate growth of pancreatic tumor in vivo.%目的 观察生长激素(GH)对胰腺癌细胞增殖及对肿瘤宿主胰岛素样生长因子Ⅰ,Ⅱ(IGF-Ⅰ,Ⅱ)-胰岛素样生长因子结合蛋白3(IGFBP3)通路的影响,初步探讨体内外实验差异的原因.方法 体外应用GH(50 ng/ml)24 h,48 h,72 h后计数胰腺癌细胞SW-1990,PANC-1及P3;选用BALB/C雌性裸小鼠35只,以SW-1990成瘤,测量瘤体积,成瘤裸鼠随机分为注射GH的实验组(GH组)及对照组(NS组),荷瘤裸鼠在最后依次注射GH后第1小时、2小时、6小时及24小时处死动物,心室穿刺抽血,切除肝脏,酶联免疫吸附法(ELISA)检测血清IGF-Ⅰ,-Ⅱ水平(P<0.05),蛋白印记(Western Blot)方法观察肝脏IGFBP3的

  14. Analysis of Impact of Treating Rats with Diabetes B and Myocardial Infarction via Carvedilol%卡维地洛对2型糖尿病合并心肌梗死大鼠治疗影响分析

    Institute of Scientific and Technical Information of China (English)

    党润芳

    2011-01-01

    目的:通过血流动力学测定研究卡维地洛对2型糖尿病合并心肌梗死大鼠治疗影响.方法:36只建立2型糖尿病合并左冠状动脉建立急性心肌梗死模型平分为两组,治疗组予卡维地洛分二次灌胃,时照组大鼠则予等量蒸馏水,观察两组血流动力学情况.结果:治疗组的主动脉收缩压(SBP)、舒张压(DBP)和左心室收缩压(LVSP)都明显少于对照组(P<0.05),有统计学意义.结论:卡维地洛能有效抑制2型糖尿病合并心肌梗死大鼠左室舒张未压,改善血流动力学和左心功能,值得在临床上尝试使用.%Objective:To prepare Naltrexone Hydrochloride Multivesicular liposomes(DepoNTX), and to study the physicochemical properties and drug release behavior in vitro.Methods:DepoNTX were prepared using multiple emulsion method. RP-HPLC was used to determine the concentration of Naltrexone, encapsulation efficiency and in vitro/in vivo release assay. The formulation and pharmaceutical process were optimized by orthogonal experimental design,with the encapsulation efficiency as the criteria. The multivesicular liposomes particles were characterized by their morphologh which was observed using light microscope, the partical size and distribution was determined with coulter counter.Results:The optimal formula was that: the concentration ratio was 1:1 of EPC and CH,the vortex time was 10s, the concentration of L-lysine was 40mM, the volume rate of the first emulsification and the second aqueous solution is 1:2.5. The partical size of DepoNTX were (5-50) μ m,themean partical size was 12.4 μ m. Naltrexone Hydrochloride was continuously released from MVL in 0.9%NaCL in vitro, and reached a maximum of 80%-85%witnin 120h. Following intramuscular injection in rats, the elimination time was more prolonged after DepoNTX injection to so lusion. Con elusion :DepoNTX was successfully encapsulated in this depot with good yield. Morphological examination using light microscope showed the

  15. Desempenho de uma semeadora-adubadora direta, em função da velocidade de eslocamento e do tipo de manejo da biomassa da cultura de cobertura do solo Performance of a seeding-fertilizer machine in function of the displacement speed and soil cover crop biomass management

    Directory of Open Access Journals (Sweden)

    Klinger B. Branquinho

    2004-08-01

    Full Text Available O objetivo do presente trabalho foi estudar o desempenho de uma semeadora-adubadora, operando em duas velocidades sobre a palhada da cultura do milheto (Pennisetum glaucum (L. R. Brown, após diferentes manejos, na semeadura direta da cultura da soja (Glycine Max L.. O experimento foi realizado com seis tratamentos, em esquema fatorial, delineados em blocos ao acaso, com quatro repetições. Os tratamentos combinaram três manejos (triturador de palhas tratorizado, rolo- faca e herbicida com duas velocidades de deslocamento da semeadora-adubadora (5,2 e 7,3 km h-1. Foram analisadas as seguintes variáveis: acúmulo e decomposição de massa seca do milheto, índice de emergência da cultura da soja, uniformidade de distribuição longitudinal de sementes, capacidade de campo efetiva da semeadora-adubadora e produtividade da soja. Os resultados mostraram que não houve diferença na decomposição da massa de milheto após os manejos e que esses, juntamente com a velocidade de deslocamento do conjunto trator-semeadora-adubadora, não influenciaram no número de dias para a emergência das plântulas de soja e na distribuição longitudinal das sementes. A capacidade de campo efetiva da semeadora-adubadora foi maior na velocidade mais alta. O rendimento de grãos não diferiu significativamente nos tratamentos estudados.The objective of the present work was to evaluate three different managements of the Pennisetum glaucum (L. R. Brown crop and two-speed displacement in a no-tillage system of soybean (Glycine max L. crop on a typical Eutrophic Red Latosol. The experiment was arranged in six treatments, in a factorial array, in a randomized complete block design, with four replications. The treatments were constituted from three combination of crop managements (straw chopper, residue coulter and herbicide and two speed displacement of the seeding-fertilizer machine (5.2 and 7.3 km h-1. The following variables have been analyzed: emergency index of

  16. The clinical application value of the reticulocytes parameters in children anemia%儿童贫血网织红细胞各参数的应用评价

    Institute of Scientific and Technical Information of China (English)

    张俏忻; 肖颖秀; 程碧珍; 林静华

    2011-01-01

    目的 探讨LH750血细胞分析仪检测患儿网织红细胞(Ret)各参数的临床意义.方法 用LH750血细胞分析仪测定120例健康体检儿童及118例贫血患儿的网织红细胞参数.结果 不同病因的贫血中,网织红细胞百分比(RET%)、网织红细胞绝对值(RET#)、未成熟网织红细胞指数(IRF)、强光散射网织红细胞百分比(HLR%)、强光散射网织红细胞绝对值(HLR#)均具有统计学意义(P<0.05).其中溶血性贫血、失血性贫血、肾性贫血、缺铁性贫血、白血病和再生障碍性贫血的HLR%分别为4.02±1.46、2.29±0.87、0.46±0.22、0.50±0.23、0.49±0.25、0.38±0.18;IRF分别为0.56±0.11、0.44±0.13、0.25±0.06、0.26±0.06、0.25±0.05、0.18±0.04.结论 网织红细胞各参数在儿童贫血疾病的诊断及疗效观察中具有较高的应用价值.%Objective To explore the clinical application value of the reticulocytes(RET)parameters in children anemia.Methods RET parameters were analyzed using Coulter LH 750 in 120 healthy children and 118 anemia children with different anemia types .Results There were significant difference (P<0.05) among the different anaemia groups in percentage of RET (RET%) ,RET absolute value (RET # ) ,high light scatter reticulocyte absolute value(HLR # ) ,percentage of high light scater reticulocyte (HLR% ) and pedo- reticulocyte index ( IRF ). The HLR% of hemolytic anemia,hemorrhagic anemia, renal anaemia,iron deficiency anemia,leukemia and aplastic anemia was 4.02±1.46、2.29±0.87、0.46±0.22、0.50±0.23、0.49±0.25 and 0.38±0.18 individually ;and the IRF was 0.56±0.11、0.44±0.13、0.25±0.06、0.26±0.06、0.25±0.05 and 0.18±0.0.Conclusion RET parameters can be of great clinically significance in diagnosis and treatment of chidrent anaemia.

  17. Stem cell harvesting protocol research in autologous transplantation setting: Large volume vs. conventional cytapheresis

    Directory of Open Access Journals (Sweden)

    Balint Bela

    2008-01-01

    Full Text Available Background/Aim. The use of peripheral blood as a source of hematopoietic stem cells (SCs is progressively increasing and has nearly supplanted bone marrow transplantation. Interpatient variability in the degree and kinetics of SC mobilization into peripheral blood is an expected event after conventional chemotherapy-based treatment, followed by sequential administration of recombinant granulocyte-colony- stimulating factor (rHu-CSF. In this study, specific factors associated with the application of two different SC-harvesting approaches, including the use of large volume leukapheresis (LVL vs. repetitive conventional apheresis (RCA, were analyzed. The basic goal of the study was to evaluate the influence of apheresis protocol (collection timing, processed blood volume and cell yield upon the clinical outcome of transplantation. Methods. Results obtained by LVL (76 pts and RCA (20 pts - control group were compared. The SC mobilizing regimen used was cyclophosphamide (4-7 g/m2 or polychemotherapy and rHuG-CSF 10-16 μg/kg of body mess (bm per day. Cell harvesting was performed using COBE-Spectra (Caridian-BCT, USA. The volume of processed blood in LVL setting was ≥ 3.5 - fold of the patient's circulating blood quantity (ranged from 12.7 to 37.8 l. All patients tolerated well the use of intensive treatment, without any side or adverse effects. Our original controlled-rate cryopreservation was carried out with 10% dimethyl sulfoxide (DMSO using Planer R203/200R or Planer 560-16 equipments (Planer Products Ltd, UK. Total nucleated cell (NC and mononuclear cell (MNC counts were examined by flow cytometry (Advia-2120 Bayer, Germany; Technicon H-3 System, USA. The CD34+ cell surface antigen was investigated by the EPICS XL-MCL device (Coulter, Germany. Results. Performing LVL-apheresis, high-level MNC and CD34+ cell yields (7.6±4.6 × 108/kg bm and 11.8±6.5 × 106/kg bm, respectively were obtained. As a result, rapid hematopoietic reconstitution

  18. The subglacial Lake Vostok (East Antarctica) surface snow is Earth-bound DNA (and dust)-free

    Science.gov (United States)

    Bulat, S.; Marie, D.; Bulat, E.; Alekhina, I.; Petit, J.-R.

    2012-09-01

    The objective was to assess the microbial cell abundance in the surface snow in Central East Antarctica and the fate of microbial genomic DNA during summer short-time exposure to surface climatic (and radiation) conditions at Vostok using flow cytometry and DNA-based methods. The surface snow (until 4m deep) was collected as clean as possible in the vicinity of the Vostok station (3 sites - courtesy of A Ekaykin and ASC Lebedev Physical Iinstitute RAS) and towards the Progress station (4 more sites with one just 29km from the coast - courtesy of A Ekaykin and S Popov) in specially decontaminated plastic crates or containers of various volumes (up to 75 kg of snow). All subsequent snow treatment manipulations (melting, concentrating, genomic DNA extraction, primary PCR set up) were performed in clean room laboratory facilities (LGGE, UJF-CNRS, Grenoble, France). Cell concentrations were determined on meltwater aliquots prepared under clean room conditions using flow cytofluorometry (Biostation, Roscoff, France). The highly concentrated meltwater (until 10000 times down) was used to extract gDNA which were subjected to bacterial 16S rRNA genes amplification in PCR and sequencing. The gDNA of a complex mesophile microbial community for exposure trials were also prepared and put onto a filter under strict clean room conditions. The filters were got exposed open to solar radiation and surface temperature at Vostok during January for various time duration periods (from 25 to 1 day). As a result no microbial cells were confidently detected in surface snow samples differed by sampling sites and people asked to collect as well. Complementary the mineral dust particle abundance did not exceed 16 mkg per liter with the particle size mode about 2.5 mkm as shown using Coulter counter. Preliminary amongst the microparticles no unusual findings (e.g. spherules of cosmic origin) were observed by shape and element composition using electron scanning microscopy. The gDNA studies

  19. 木豆叶总芪对高脂模型兔的降脂作用及调节机制%Hypocholesterolemic effect of total stilbene from Cajanus cajan L.in hyperlipidemic rabbit model and regulatory mechanism

    Institute of Scientific and Technical Information of China (English)

    郭显蓉; 骆庆峰; 康晓敏; 尹洪超; 斯建勇

    2011-01-01

    mol/L, 3 μmol/L, 0.3 μ mol/L) or 17β-estradiol ( 1 μ mol/L).The macrophages were washed with PBS again and incubated in the serum-free RPMI.1640 medium containing apoA-I( 10 μg/mL) for 12 hours.The radioactivity of samples of both supernatants and cell lysates were determined using the liquid scintillation counter ( Beckman, LS 6000SC, USA).Fractional cholesterol efflux was calculated, and the ABCA1 protein was quantified by Western blot.Results In TSC(200 mg/kg) group,the serum and hepatic TC were reduced by 35.36% and 35.97%(P <0.01 ) ;the TG contents of serum and liver were also lowered by 12.34% (P<0.05) and 41.32% (P <0.01).At the same time, serum LDL-C decreased by 29.07% (P < 0.01 ).The cholesterol effiux percentage from macrophage was increased by 55.39% and 24.97% (P <0.01 ) respectively in CH and CM group.The expression of the ABCA1 was significanfiy increased in CH group.Conclusion TSC has potential hypolipidemic effects, possibly via up-regulating the ABCA1 protein expression, subsequenfiy resulting in increased macrophage cholesterol effiux and RCT.

  20. In vitro release of 1,3-bis (2-chloroethyl)-1-nitrosourea sustained-release microspheres and the distribution in rat brain tissues

    Institute of Scientific and Technical Information of China (English)

    Xia Li; Liping Guo; Qin Li

    2006-01-01

    BACKGROUND: The implantation of released chemotherapeutic drugs, which takes biodegradable polymer as vector, into the tumor site can get high concentration and release the drug for a long time, it can directly act on the tumor cells, and reduce the general toxicity.OBJECTIVE: To explore the in vitro and in vivo course of 1,3-bis (2-chloroethyl)-1-nitrosourea (BCNU) sustained-release from BCNU-loaded polylactide (PLA) microspheres (MS) and location in rat brain tissue.DESIGN: A repetitive measurement.SETTING: Central Pharmacy, General Hospital of Chinese People's Armed Police Forces.MATERIALS: Thirty male SD rats were used. PLA (Mr5000, batch number: KSL8377) was produced by Wako Pure Chemical Inc.,Ltd. (Japan); BCNU (batch number: 021121) by Tianjin Jinyao Amino Acid Co., Ltd.;BCNU-PLA-MS was prepared by the method of solvent evaporation and pressed into tablets (10 mg/tablet).High-performance liquid chromatography (HPLC) Agilent 1100 (USA); LS9800 liquid-scintillation radiometric apparatus (Beckman). Chromatographic conditions: Elite Hypersil ODS2 C18 chromatographic column (5 μm,4.6 mm ×150 mm); Mobile phase: methanol: water (50:50), flow rate was 1.0 mL per minute, wave length of ultraviolet detection was 237 nm, and the inlet amount of samples was 10 μL.METHODS: The experiments were carried out in the experimental animal center of the General Hospital of Chinese Armed Police from May 2004 to July 2005. ① In vitro BCNU-PLA-MS release test: BCNU-PLA-MS was prepared by the method of solvent evaporation, then placed in 0.1 mol/L phosphate buffered solution (PBS, pH 7.4, 37 ℃), part of MS were taken out at 1, 2, 3, 7, 10 and 15 days respectively, and the rest amount of BCNU in MS was determined by HPLC, then the curve of BCNU-PLA-MS release was drawn. ②In vivo BCNU-PLA-MS release and distribution test: The rats were anesthetized, then BCNU-PLA-MS were implanted to the site 1 mm inferior to the cortex of frontal lobe. Five rats were killed postoperatively

  1. A special year, thanks and a look ahead

    Science.gov (United States)

    Birch, David J. S.

    2014-01-01

    Dear authors, reviewers and readers of Measurement Science and Technology , I would like to thank all those who have published papers with us in 2013, and offer a special thanks to those of you who have given up much of your precious time and kindly reviewed articles for the journal. 2013 was of course a very special year for the journal, marking the 90th anniversary of the journal's foundation, as Journal of Scientific Instruments. In order to mark the anniversary we started the year with a historical perspective of the journal [1], and a historical review of 90 years of dielectric properties measurement of materials [2]. The celebrations continued with a stimulating meeting at the Institute of Physics in London in March, entitled 'Frontiers of Measurement'. In keeping with the multidisciplinary ethos of the journal, the speakers covered a diverse range of topics, all linked by the common language of measurement science. Patrick Gill of NPL talked about atomic clocks, Bruce Tromberg of the Beckman Laser Institute and Medical Clinic covered biophotonics, Roger Bisby of the University of Salford lectured on developments in biochemical techniques, Ivan Marusic of the University of Melbourne spoke on fluids and Mervyn Miles of the University of Bristol described recent developments in atomic force microscopy. Nicola Gulley, Editorial Director of IOP Publishing (IOPP), briefed the meeting on some of the latest developments in the world of scientific publishing. The meeting ended with a presentation to Sharon D'Souza, outgoing Publisher of the journal, and a welcome for Ian Forbes who has taken over the role. On behalf of all the Editorial Board I would like to offer a very special thanks to Sharon for all her great work for the journal over many years and wish her every success in her new IOPP posts as Publisher of Reports in Progress in Physics and the recently launched Methods and Applications in Fluorescence. Ian Forbes comes to the journal with a PhD in Physics

  2. Changes of biochemical indexes in patients with acute cerebral infarction after treatment with simvastatin

    Institute of Scientific and Technical Information of China (English)

    Cheng Yang

    2006-01-01

    BACKGROUND: At present, it is believed that the important causes of cerebral infarction are the disorders of lipid metabolism and endothelial function, and the outcomes of clinical treatment can be improved by regulating serum lipids and antiinflammation, etc.OBJECTIVE: To observe the effect of simvastatin, inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, on the levels of serum lipids, serum enzymic indexes and inflammatory metabolic indexes in patients with cerebral infarction.DESIGN : A comparative observation.SETTING: Department of Geriatrics, Longquanshan Hospital of Liuzhou City.PARTICIPANTS: Forty-eight patients with acute cerebral infarction wera selected from the Department of Geriatrics of Longquanshan Hospital of Liuzhou from March 2004 to February 2006, including 24 males and 24females, the mean age was (54±12) years, average disease course was (10.0±4.5) days. They were all accorded with the diagnostic standard for cerebral infarction set by the Fourth National Academic Meeting for Cerebrovascular Disease in 1999, and cerebral hemorrhage was excluded by cranial CT scanning. The 48patients were randomly divided into control group (n =24) and traatment group (n =24). Informed consents were obtained from all the participants.METHODS: ① All the patients wera treated according to the symptoms, besides those in the traatment group were given simvastatin (Harbin Pharm. Group Sanjing Pharmaceutical Shareholding, Co.,Ltd., No. H20010454;Batch number: 20040218; 5 mg/tablet). The initial dosage was 10 mg per day for 4 weeks, and then increased to 30 mg per day for another 4 weeks. ② Before treatment and within 1 week after treatment, the total cholesterol, triglyceride, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST), craatine kinase and C raactive protein in serum were determined with Beckman-cx7 automatic biochemical analytical apparatus in both groups. ③ The

  3. Assessment of the performance of red blood cells folic acid analyzer%红细胞叶酸检测仪性能评价

    Institute of Scientific and Technical Information of China (English)

    杨桂花; 胡梅; 韩静; 张曼

    2013-01-01

    目的通过对国产红细胞叶酸检测仪进行性能分析,以验证其检测能力是否符合行业标准。方法参考美国临床和实验室标准化协会 EP5-A2文件,通过20 d 测定2个水平质控物,获得精密度实验数据,计算批内、批间和实验室内变异系数(CV)并与生物学变异规定的要求相比较;按照EP9-A2文件,通过与Beckman Couter UniCel DxI800全自动免疫分析仪测定结果比对验证仪器的正确度;参照 EP6-A文件,用多项式回归法进行线性评价;通过测定20例能够合理代表健康总体的样本对仪器给定的参考范围进行验证。结果批内CV小于6.67%(1/4生物学变异允许总误差),批间 CV小于8.90%(1/3生物学变异允许总误差),实验室内CV小于13.35%(1/2生物学变异允许总误差);在医学决定水平处预期偏倚的可信区间小于允许的误差范围;二次和三次多项式系数与0比较差异均无统计学意义,检测系统呈线性;厂家给定的参考范围不适合本实验所在地人群。结论国产红细胞叶酸检测仪精密度、正确度和线性符合要求;实验室应建立适合当地人群的参考范围。%Objective Through performance analysis of domestic red blood cells folic acid instrument to verify its testing ability. Methods According to national committee for clinical laboratory standards(NCCLS)EP5-A2,the precision was obtained through two level quality product detection within 20 days then the intra-assay variation coefficient(CV),inter-assay CV and CV in labora-tory was calculated and compared with the precision requirement coming from the red blood cells folic acid biological variation.Ac-cording to EP6-A,the linear evaluation was investigated with method of polynomial regression.By measuring the red blood cells fo-lic acid from 20 persons who presented the total healthy people,the reference scope given by the manufacturer was verified

  4. Editorial - [FSRJ]Future Studies Research Journal - V6N2

    Directory of Open Access Journals (Sweden)

    Renata Giovinazzo Spers

    2014-12-01

    éticos do país a partir da maior utilização de gases combustíveis para o aquecimento residencial de água. Aprovado no Fast Track do Semead 2014, o artigo “Quantificação dos planos estratégicos por meio do orçamento empresarial: uma aplicação prática com métodos estocásticos” os autores Marino Luiz Eyerkaufer, Janaina Poffo Possamai, Mirian Buss Conçalves, apresentam neste estudo uma aplicação de métodos estocásticos em que o probabilismo se demonstra como alternativa de minimização das incertezas quanto à assertividade das estimativas. Boa leitura!James Terence Coulter WrightEditorRenata Giovinazzo SpersEditora Adjunta

  5. Preparo do solo em áreas de produção de grãos, silagem e pastejo: efeito na resistência tênsil e friabilidade de agregados Soil tillage in grain and silage-producing areas and pasture: effect on tensile strength and aggregate friability

    Directory of Open Access Journals (Sweden)

    Marina Araújo Bavoso

    2010-02-01

    ção da F. Os resultados demonstraram que a RT foi influenciada pelos sistemas de produção e tipos de preparo do solo. O menor valor de RT foi verificado no solo sob Pastejo, enquanto o Sistema Plantio Direto apresentou o maior valor de RT. A friabilidade não distinguiu os sistemas de produção e tipos de preparo de solo estudados.The understanding and quantification of soil use and management on the soil physical quality are important to develop and select sustainable agricultural systems. The purpose of this study was to evaluate the structural quality of a clayey Brazilian Oxisol under different production and soil tillage systems by measuring the tensile strength (TS and to quantify aggregate friability (F. The study was conducted in Castro, Paraná state, in southern Brazil, using three production systems and seven soil tillage types, in a randomized block design. The following production systems were evaluated: (PS I - ryegrass for soil cover (winter and corn for grain production (summer; (PS II ryegrass for haylage/silage and corn for grain production; and (PS III - ryegrass for grazing and corn for grain production. Seven types of soil tillage were evaluated in each system: (G1 - plow harrow in winter, depth 0.15 m; (G2 - plow harrow in winter and summer, depth 0.15 m; (Plow - disk plow, depth 0.20 m; (Laser wing subsoiler - subsoiler wing tips, to a depth of 0.45 m; (Subsoiler - subsoiler, depth 0.80 m; (Aerator - soil aerater Aeromix®; and (NT - No-tillage, where ryegrass was sown using a double disc and corn with a coulter. From each experimental plot soil blocks (0.20 x 0.15 x 0.07 m were taken which were broken up by hand in their natural aggregates. Then, the aggregates were air-dried for 24 h and passed through sieves with diameters of 12.5 and 19 mm. Forty aggregates from each block were selected and tested for indirect tension to determine TS and quantify F. TS was influenced by the production systems and soil tillage types. The lowest and the

  6. Editorial - [FSRJ]Future Studies Research Journal - V7N1

    Directory of Open Access Journals (Sweden)

    Renata Giovinazzo Spers

    2015-08-01

    artigo que foi aprovado no Fast Track do III SINGEP. A análise desta pesquisa pode auxiliar na construção do planejamento estratégico de uma pequena empresa em fase de crescimento para que seus objetivos estratégicos sejam atingidos.“Aplicação da Criatividade para Gerar Projetos de Inovação: O Caso Prático de uma Estratégia Didática” também foi aprovado no Fast Track III SINGEP. Os autores: Eliana Maria dos Santos Pereira Alves e Ovídio Felippe Pereira da Silva Júnior discutem elementos como identificação de oportunidades, criatividade, inovação e empreendedorismo, buscando sua inter-relação para compreender como é possível motivar a geração de propostas de projetos inovadores em ambientes de ensino.Os autores Mariana Bassi Sutter, Laura Ferranty Mac Lennan, Gabriela Pelegrini Tiscoski e Edison Fernandes Polo, abordam o tema de brasilidade com o objetivo de compreender as representações da imagem do Brasil e os diversos significados do conceito de brasilidade por diferentes pontos de vista.Competitividade Agressiva é o tema abordado pelo autor Antônio Benedito Oliveira Junior e especificamente, demostra a importância da CA para a sobrevivência e crescimento da empresa em mercados cada vez mais dinâmicos, turbulentos e competitivos.O artigo “A Influência do Nível de Competências e de Maturidade em Gestão de Projetos no Resultado Corporativo de uma Empresa do Setor de Transformação” de autoria de Gislaine Cristina dos Santos Teixeira, Emerson Antônio Maccari e Daniel Simonsen, tem por objetivo analisar a influência das competências e da maturidade em gestão de projetos das equipes matriciais e dos gerentes no desempenho dos projetos e consequentemente no resultado corporativo. Boa leitura! Renata Giovinazzo SpersEditora Científica James Terence Coulter WrightEditor Internacional 

  7. Phase 1 Final Technical Report - MgB2 Synthesis for High Field Performance

    Energy Technology Data Exchange (ETDEWEB)

    Mohit Bhatia; Peter McIntyre

    2009-11-02

    boron results in the formation of parasitic phases such as MgB4, MgB7, etc. Such parasitic phases are a primary element of the connectivity problem, in which even though a sample powder may contain grains of high-quality MgB2, adjacent grains are surrounded by intergrowths of parasitic phases so that current trans-port is badly degraded. The best results to date have been obtained using boron powder produced long ago for a rocket propellant development project. The synthesis process was complex and is now largely lost, and the manufacturing equipment has long since been scrapped. The last batch of the powder has been used during recent years to support MgB2 R&D at several labs, but supplies are dwindling. ATC has identified a first application of its plasma torch to synthesize phase-pure amorphous boron flake using a rapid-quench splat technique. Inexpensive technical-grade boron would be purified of contaminants, then dispersed as an aerosol in inert gas and passed through the plasma torch to melt it into a spray. The spray would be splat-condensed on a rotating drum to form pure amorphous flake. The process would begin with technical-grade boron powder, having good stoichiometric purity, nanoscale particles, but significant contamination of MgO and crystalline boron. We used wet chemistry to remove B2O3 completely and reduced the MgO impurity, and analyzed the particle size distribution using a Coulter counter and the phase composition using X-ray diffrac-tion (XRD). The next step will be to build an rf plasma torch with a recirculating single-component aerosol feed and the cooled splat drum and collector, and undertake process devel-opment for amorphous boron powder. This revised goal has two benefits. First, it is an easier technology than our ultimate goal of a multi-component laminar flow torch. We have been counseled by those experienced in plasma torch technology that our ultimate goal will require a torch that should be feasible but has never been attempted. It

  8. Increasing energy efficiency by geometric modification of hoe-type furrow opener

    Directory of Open Access Journals (Sweden)

    R Rahimzadeh

    2016-09-01

    Full Text Available Introduction Direct planting becomes more common in the recent years, because it conserves soil and water as well as it saves energy and time. However, this technology needs special implements such as seed planter. Given that direct planting is practiced in undisturbed lands, so it was needed to design a special furrow opener. In order to obtain a suitable furrow opener this experiment was conducted in rain-fed Agricultural Research Institute in Maragheh. Materials and Methods Most of seed planters that are used for cultivation in rain fed conditions are equipped by hoe-type furrow opener. Hoe-type furrow openers have good penetration in hard and dry soils. However, they do not have the ability for direct planting. Hoe-type furrow opener was chosen as a model. Then by changing the geometric form of the depth to width ratio (d/w, the two openers were designed. In the first design, which was called O1 two wings and a narrow blade acting as a coulter were added in front of the hoe-type furrow opener. In the second design, which was called O2, in addition to the O1 modification, furrow opener width was decreased and a disk blade was added for seed sowing (Fig. 1. The performance of O1 and O2 openers were compared with the conventional hoe-type furrow opener (check in soil bin and in field conditions. At three different forward speeds (1, 1.5 and 2 m.s-1 with 3 replications, the effects of the openers designs of vertical and horizontal soil forces were evaluated in soil-bin conditions. In order to evaluate the performance of the furrow opener in field conditions, an experiment was conducted using a split plot design based on RCBD at 4 replications. Furrow openers formed the main plots and forward speeds formed the sub plots. Each plot size was 22 meters long in two rows for each treatment. After germination of wheat crop, the numbers of seedlings in two rows were counted (along a one meter. After crop maturity, all plots were harvested by hand and

  9. Evaluation of modified hemodilution combined therapy in the treatment of acute ischemic stroke in the elderly

    Institute of Scientific and Technical Information of China (English)

    Yue Chen; Guangbai Xie

    2006-01-01

    ) was made, twice a day, 1 bag once. At the same time, the above-mentioned dextran-40 liquid of 500 mL was intravenously injected, once a day, 14 days in total; On the 6th day after therapy, the above-mentioned aseptic autoblood stored in refrigerator at 4 ℃ was transfused back into the patients following pre-treatment of high-concentration oxygenation and ultraviolet irradiation by light quantum instrument. Patients of control group were intravenously injected of 0.4 g venoruton(Traditional Chinese medicine compound parenteral solution for promoting blood circulation and removing blood stasis ) and 50 g/L glucose of 500 mL, 75 mg coagulation index, change of platelet aggregation rate and change of hemorrheology of patients in two groups were monitored before and after therapy. The level of blood lipid of patients in two groups was measured with American Beckman automatic biochemistry analyzer. Blood flow rate of middle cerebral artery of resting electrocardiogram were measured with American HP SONOS 2500 sonoscope. Neuro-dysfunction score revised in the national conference (1995) was used to evaluate the recovery of neurological function effect were observed at the same time.effect of drug.RESULTS: Totally 90 patients were enrolled in the experiment. One patient from treated group died of hyperosmolar nonketotic diabetic coma of complicated diabetes mellitus. One patient from control group died ulation index and platelet aggregation rate: prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT) of patients after therapy were significantly longer than those before therapy in the treated group and those after therapy in control group [After therapy in treated group: (18.4±1.9),(41.8±2.1), (19.7±1.7)s, Before therapy in treated group: (13.4±1.3), (35.8±1.3), (12.5±0.9)s, After therapy in control group: (16.9±1.5), (39.1±1.1), (11.9±2.1) s, P < 0.05] ;Concentration of fibrinogen (Fbg) after therapy was significantly lower than

  10. Preface: SciDAC 2009

    Science.gov (United States)

    Simon, Horst

    2009-07-01

    and posters goes to the teams of researchers, the success of this year's conference is due to the strong efforts and support from members of the 2009 SciDAC Program Committee and Organizing Committee, and I would like to extend my heartfelt thanks to them for helping to make the 2009 meeting the largest and most successful to date. Program Committee members were: David Bader, LLNL; Pete Beckman, ANL; John Bell, LBNL; John Boisseau, University of Texas; Paul Bonoli, MIT; Hank Childs, LBNL; Bill Collins, LBNL; Jim Davenport, BNL; David Dean, ORNL; Thom Dunning, NCSA; Peg Folta, LLNL; Glenn Hammond, PNNL; Maciej Haranczyk, LBNL; Robert Harrison, ORNL; Paul Hovland, ANL; Paul Kent, ORNL; Aram Kevorkian, SPAWAR; David Keyes, Columbia University; Kwok Ko, SLAC; Felice Lightstone, LLNL; Bob Lucas, ISI/USC; Paul Mackenzie, Fermilab; Tony Mezzacappa, ORNL; John Negele, MIT; Jeff Nichols, ORNL; Mike Norman, UCSD; Joe Oefelein, SNL; Jeanie Osburn, NRL; Peter Ostroumov, ANL; Valerio Pascucci, University of Utah; Ruth Pordes, Fermilab; Rob Ross, ANL; Nagiza Samatova, ORNL; Martin Savage, University of Washington; Tim Scheibe, PNNL; Ed Seidel, NSF; Arie Shoshani, LBNL; Rick Stevens, ANL; Bob Sugar, UCSB; Bill Tang, PPPL; Bob Wilhelmson, NCSA; Kathy Yelick, NERSC/LBNL; Dave Zachmann, Vista Computational Technology LLC. Organizing Committee members were: Communications: Jon Bashor, LBNL. Contracts/Logistics: Mary Spada and Cheryl Zidel, ANL. Posters: David Bailey, LBNL. Proceedings: John Hules, LBNL. Proceedings Database Developer: Beth Cerny Patino, ANL. Program Committee Liaison/Conference Web Site: Yeen Mankin, LBNL. Tutorials: David Skinner, NERSC/LBNL. Visualization Night: Hank Childs, LBNL; Valerio Pascucci, Chems Touati, Nathan Galli, and Erik Jorgensen, University of Utah. Again, my thanks to all. Horst Simon San Diego, California June 18, 2009

  11. 职业性接触灭蚁药物对人体免疫功能的影响%Impacts of occupational contact with termite-control drug on human immune function

    Institute of Scientific and Technical Information of China (English)

    刘霞; 朱宝立; 班永宏; 陈敏

    2004-01-01

    BACKGROUND: Many chemical poisons existing in our daily life and environment have immunotoxicity in certain dose including termite-control drug, chlordane, and pesticides containing arsenic. Whether occupational contact with this type of chemical poisons would induce injuries of the immune system of the operators?OBJECTIVE: To investigate the impacts of occupational contact with termite-control drug on the immune functions of the operators.DESIGN: A case-control trial.SETTINGS: Original Department of Occupational Diseases, Toxic and Chemical Laboratory, Jiangsu Hospital of Prevention and Treatment for Occupational Disease.PARTICIPANTS: The study was performed in the original Department of Occupational Diseases, Toxic and Chemical Laboratory, Jiangsu Hospital of Prevention and Treatment for Occupational Disease from January 2002 and December 2003. Contact groups were termite-control group, arsenic-producing group and chlordane-producing group. A total of 216 subjects were selected from the staff who were termite controllers of some termite-control institute, workers produce arsenic drug and chlordane of some pesticide factory. Another 64 healthy subjects who had no occupational in the control group were healthy volunteers had no contact with toxic and harmful substances, other conditions of the control group were similar to that contacts with toxic and harmful substances but other diseases.INTERVENTIONS: ICS apparatus for immune chemistry system made by Beckman Company(USA) and PEG-ultraviolet quantitative method were used for the assay of serum IgA, IgG, IgM, C3, C4, CRP and CIC of operators. The impacts of the length of services, smoking, drinking and the poison concentration in vivo on immune level were analyzed as well.MAIN OUTCOME MEASURES: Serum levels of IgG, IgA, IgM, C3,C4, CRP and CIC.RESULTS: Occupational contacts of termite-control drug significantly reduced serum IgG, IgA and IgM levels( t =2.16 to 2. 35, P < 0. 05); However, there were no significant

  12. Preface: SciDAC 2008

    Science.gov (United States)

    Stevens, Rick

    2008-07-01

    Microsoft Research at a new conference facility in Redmond, Washington. Over 90 people attended the tutorials, which covered topics ranging from an introduction to BG/P programming to advanced numerical libraries. The SciDAC and INCITE programs and the DOE Office of Advanced Scientific Computing Research core program investments in applied mathematics, computer science, and computational and networking facilities provide a nearly optimum framework for advancing computational science for DOE's Office of Science. At a broader level this framework also is benefiting the entire American scientific enterprise. As we look forward, it is clear that computational approaches will play an increasingly significant role in addressing challenging problems in basic science, energy, and environmental research. It takes many people to organize and support the SciDAC conference, and I would like to thank as many of them as possible. The backbone of the conference is the technical program; and the task of selecting, vetting, and recruiting speakers is the job of the organizing committee. I thank the members of this committee for all the hard work and the many tens of conference calls that enabled a wonderful program to be assembled. This year the following people served on the organizing committee: Jim Ahrens, LANL; David Bader, LLNL; Bryan Barnett, Microsoft; Peter Beckman, ANL; Vincent Chan, GA; Jackie Chen, SNL; Lori Diachin, LLNL; Dan Fay, Microsoft; Ian Foster, ANL; Mark Gordon, Ames; Mohammad Khaleel, PNNL; David Keyes, Columbia University; Bob Lucas, University of Southern California; Tony Mezzacappa, ORNL; Jeff Nichols, ORNL; David Nowak, ANL; Michael Papka, ANL; Thomas Schultess, ORNL; Horst Simon, LBNL; David Skinner, LBNL; Panagiotis Spentzouris, Fermilab; Bob Sugar, UCSB; and Kathy Yelick, LBNL. I owe a special thanks to Mike Papka and Jim Ahrens for handling the electronic theater. I also thank all those who submitted videos. It was a highly successful experiment. Behind the

  13. Without 'Focus'

    Directory of Open Access Journals (Sweden)

    Aldo Sevi

    2010-12-01

    Full Text Available It is widely accepted that a notion of 'focus', more or less as conceived of in Jackendoff (1972, must be incorporated into our theory of grammar, as a means of accounting for certain observed correlations between prosodic facts and semantic/pragmatic facts. In this paper, we put forth the somewhat radical idea that the time has come to give up this customary view, and eliminate 'focus' from our theory of grammar. We argue that such a move is both economical and fruitful.Research over the years has revealed that the correlations between prosody, 'focus', and the alleged semantic/pragmatic effects of focus are much less clear and systematic than we may have initially hoped. First we argue that this state of affairs detracts significantly from the utility of our notion of 'focus', to the point of calling into question the very motivation for including it in the grammar. Then we look at some of the central data, and show how they might be analyzed without recourse to a notion of 'focus'. We concentrate on (i the effect of pitch accent placement on discourse congruence, and (ii the choice of 'associate' for the so-called 'focus sensitive' adverb only. We argue that our focus-free approach to the data improves empirical coverage, and begins to reveal patterns that have previously been obscured by preconceptions about 'focus'.ReferencesBeaver, D. & Clark, B. 2008. Sense and Sensitivity: How Focus Determines Meaning. Blackwell.Beaver, D., Clark, B., Flemming, E., Jaeger, T. F. & Wolters, M. 2007. ‘When semantics meets phonetics: Acoustical studies of second occurrence focus’. Language 83.2: 245–76.http://dx.doi.org/10.1353/lan.2007.0053Beckman, M. & Hirschberg, J. 1994. ‘The ToBI Annotation Conventions’. Ms.,http://www.cs.columbia.edu/~julia/files/conv.pdf.Bolinger, D. 1972. ‘Accent is predictable (if you are a mind-reader’. Language 48.3: 633–44.http://dx.doi.org/10.2307/412039Büring, D. 2006. ‘Focus projection and default

  14. Browning boreal forests of western North America

    Science.gov (United States)

    Verbyla, David

    2011-12-01

    's boreal forest Remote Sens. 2 2729-47 Riordan B, Verbyla D and McGuire A D 2006 Shrinking ponds in subarctic Alaska based on 1950-2002 remotely sensed images J. Geophys. Res. 111 G04002 Ruess R W, McFarland J M, Trummer L M and Rohrs-Richey J K 2009 Disease-mediated declines in N-fixation inputs by Alnus tenuifolia to early-successional floodplains in interior and south-central Alaska Ecosystems 12 489-502 Stafford J M, Wendler G and Curtis J 2000 Temperature and precipitation of Alaska: 50 year trend analysis Theor. Appl. Climatology 67 33-44 Stow D, Peterson A, Hope A, Engstrom R and Coulter L 2007 Greenness trends of Arctic tundra vegetation in the 1990s: comparison of two NDVI data sets from NOAA AVHRR systems Int. J. Remote Sens. 28 4807-22 van Mantgem P J et al 2009 Widespread increase of tree mortality rates in the western United States Science 323 521-4 Walsh, J E, Chapman W L, Romanovsky V, Christensen J H and Stendel M 2008 Global climate model performance over Alaska and Greenland J. Clim. 21 6156-74 Wendler G and Shulski M 2009 A century of climate change for Fairbanks, Alaska Arctic 62 295-300 Zhang K, Kimball J S, Hogg E H, Zhao M, Oechel W C, Cassano J J and Running S W 2008 Satellite-based model detection of recent climate-driven changes in northern high-latitude vegetation productivity J. Geophys. Res. 113 G03033

  15. only: A Case Study In Projective Meaning

    Directory of Open Access Journals (Sweden)

    Craige Roberts

    2010-12-01

    Full Text Available I offer an integrated theory of the meaning of only in which the prejacent, while not presupposed, is both entailed and backgrounded, hence tends to project (following a general proposal about projection due to Simons et al. 2010. Moreover, I argue, contra Beaver & Brady (2008, that only is not conventionally associated with focus, the focus effects arising instead pragmatically. But I do adopt aspects of their semantics for only, including the presupposition of a pre-order over the elements of its domain.ReferencesAbbott, B. 2000. ‘Presuppositions as non-assertions’. Journal of Pragmatics 32: 1419–37.http://dx.doi.org/10.1016/S0378-2166(9900108-3Abbott, B. 2008. ‘Presuppositions and common ground’. Linguistics and Philosophy 31: 523–538.http://dx.doi.org/10.1007/s10988-008-9048-8Amaral, P. 2010. ‘Entailment, assertion, and textual coherence: the case of almost and barely’. Linguistics 48, no. 3: 525–545.http://dx.doi.org/10.1515/LING.2010.016Amaral, P., Roberts, C. & Allyn Smith, E. 2008. ‘Review of The Logic of Conventional Implicature by Chris Potts’. Linguistics and Philosophy 30: 707–749.http://dx.doi.org/10.1007/s10988-008-9025-2Atlas, J. D. 1993. ‘The importance of being “only”: testing the neo-Gricean versus neoentailment paradigms’. Journal of Semantics 10: 301–18.http://dx.doi.org/10.1093/jos/10.4.301Beaver, D. 2001. Presupposition and Assertion in Dynamic Semantics. CSLI Publications, Stanford, CA.Beaver, D. 2010. ‘Handout from a talk on projective meaning’. Stuttgart, Germany.Beaver, D. & Brady, C. 2003. ‘Always and Only: Why not all focus sensitive operators are alike’. Natural Language Semantics 11: 323–62.http://dx.doi.org/10.1023/A:1025542629721Beaver, D. & Brady, C. 2008. Sense and Sensitivity. Blackwell.http://dx.doi.org/10.1002/9781444304176Beckman, M. E. & Ayers, G. M. 1994. ‘Guidelines for ToBI labeling guide, ver. 2.0’. On-line at The Ohio State University: www