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Sample records for batata solanum tuberosum

  1. Powdery scab effect on the potato Solanum tuberosum ssp. andigena growth and yield Efeito da sarna pulverulenta no desenvolvimento e produtividade da batata Solanum tuberosum ssp. andigena

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    Elizabeth Gilchrist; Juliana Soler; Ueli Merz; Sebastian Reynaldi

    2011-01-01

    Powdery scab affects most potato production zones in the world. The causal organism Spongospora subterranea f. sp. subterranea forms galls in roots and lesions on tubers during its replication. Recent researches suggest that S. subterranea could cause harmful effects on the host plant and potentially on yield.In order to quantify the disease impact on plant growth and yield, potato Solanum tuberosum ssp. andigena, cv. Diacol Capiro was grown in a soil infested with S. subterranea and compared...

  2. Soma térmica de algumas fases do ciclo de desenvolvimento da batata (Solanum tuberosum L. Thermal time of some developmental phases in potato (Solanum tuberosum L.

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    Fabiana Luiza Matielo de Paula

    2005-10-01

    Full Text Available O método da soma térmica tem sido usado para representar o efeito da temperatura do ar sobre o crescimento e desenvolvimento das plantas, por ser um método simples e uma melhor medida de tempo biológico do que dias do calendário civil ou dias após a semeadura. O objetivo deste trabalho foi determinar a soma térmica por diferentes métodos de cálculo para as fases de emergência ao início da tuberização, do início da tuberização ao início da senescência e do início da senescência à colheita da batata cultivar Asterix, cultivada a campo. Experimentos foram conduzidos em Santa Maria, RS, com onze datas de plantio durante 2003 e 2004. O delineamento utilizado foi blocos ao acaso, com 4 repetições. A soma térmica para as diferentes fases variou com a época de plantio, com o método de cálculo e com as temperaturas cardinais. Estes resultados indicam que a soma térmica deve ser usada com cautela como medida de tempo biológico em batata.Thermal time has been used to represent the effect of the air temperature on plants growth and development. Thermal time is a simple method and a better time descriptor than calendar days in plants. The objective of this study was to calculate the thermal time by different calculation methods for different developmental phases (emergency to tuber initiation, from tuber initiation to beginning of senescence and from beginning of senescence to harvest time in field grown potato cultivar Asterix. Field experiments were carried out at Santa Maria RS, Brazil, with eleven planting dates during 2003 and 2004. The experimental design was a complete randomized block design with four replications. Thermal time for different developmental phases varied according to planting date, calculation method, and cardinal temperatures. These results indicate that the thermal time should be used with caution to tell time in potato.

  3. Efeito da substituição parcial da farinha de trigo por farinha de casca de batata (Solanum Tuberosum Lineu) Effect of the partial replacement of wheat flour for potato skin flour (Solanum Tuberosum L.)

    OpenAIRE

    Anderson Felicori Fernandes; Joelma Pereira; Rogério Germani; João Oiano-Neto

    2008-01-01

    O aproveitamento dos subprodutos da agroindústria de alimentos diminui os custos da produção, aumenta o aproveitamento total do alimento e reduz o impacto que esses subprodutos podem causar ao serem descartados no ambiente. Dessa forma, alguns subprodutos da batata são aproveitados e transformados em ingredientes alimentícios, como é o caso da casca. Neste estudo utilizou-se a casca de batata para produção de farinha visando a sua utilização em produtos de panificação, sendo utilizadas as seg...

  4. Efeito da substituição parcial da farinha de trigo por farinha de casca de batata (Solanum Tuberosum Lineu Effect of the partial replacement of wheat flour for potato skin flour (Solanum Tuberosum L.

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    Anderson Felicori Fernandes

    2008-12-01

    Full Text Available O aproveitamento dos subprodutos da agroindústria de alimentos diminui os custos da produção, aumenta o aproveitamento total do alimento e reduz o impacto que esses subprodutos podem causar ao serem descartados no ambiente. Dessa forma, alguns subprodutos da batata são aproveitados e transformados em ingredientes alimentícios, como é o caso da casca. Neste estudo utilizou-se a casca de batata para produção de farinha visando a sua utilização em produtos de panificação, sendo utilizadas as seguintes porcentagens na composição da farinha mista: 0-50-50%; 3-47-50%; 6-44-50%; 9-41-50% e 12-38-50% de farinha de casca de batata, farinha de trigo branca e farinha de trigo integral, respectivamente. Foram realizadas análises físico-químicas na farinha de casca de batata, análises físicas na farinha de casca, nas farinhas de trigo branca e integral e análises reológicas nas farinhas mistas. A farinha de casca de batata apresentou bons teores de fibras e minerais, e dentro deste último, destacam-se os teores de fósforo, cálcio e magnésio. Quanto à granulometria e à diferença de cor, as farinhas de casca de batata e a farinha de trigo integral apresentaram valores mais próximos uma da outra. À medida que se aumentou a substituição da farinha de trigo branca pela farinha de casca de batata, as análises reológicas indicaram uma queda na qualidade tecnológica das massas.Reusing some agro-industrial food by-products lower the production costs, increase efficient food utilization, and reduce the impact that these sub-products cause if discarded in the environment. Thus, some potato by-products are reused utilized and transformed into food ingredients, such as potato skin. In this study, potato skin was utilized for the production of flour to be used in bakery products. The following percentages were utilized in the composition of the mixed flour: 0-50-50%; 3-47-50%; 6-44-50%; 9-41-50%, and 12-38-50% of potato skin flour, white

  5. Podridão-mole em pós-colheita de batata (Solanum tuberosum) incitada por Pseudomonas viridiflava Post hervest soft rot in potabo (Solanum tuberosum) induced by Pseudomonas viridiflava

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    Dirceu Macagnan; Reginaldo da Silva Romeiro; Davi Mesquita de Macedo; Daniel Augusto Schurt

    2007-01-01

    Isolou-se uma bactéria incitadora de podridão-mole em batata e procurou-se identificá-la em nível de espécie. Testes biológicos, bioquímicos e tintoriais permitiram posicionar o microrganismo em questão com pertencente à espécie Pseudomonas viridiflava. Procurou-se também investigar a suscetibilidade de diferentes órgãos de reserva de distintas espécies botânicas à espécie bacteriana. Este trabalho mostra e confirma que outras espécies que não as de Pectobacterium spp. são capazes de incitar ...

  6. Podridão-mole em pós-colheita de batata (Solanum tuberosum incitada por Pseudomonas viridiflava Post hervest soft rot in potabo (Solanum tuberosum induced by Pseudomonas viridiflava

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    Dirceu Macagnan

    2007-09-01

    Full Text Available Isolou-se uma bactéria incitadora de podridão-mole em batata e procurou-se identificá-la em nível de espécie. Testes biológicos, bioquímicos e tintoriais permitiram posicionar o microrganismo em questão com pertencente à espécie Pseudomonas viridiflava. Procurou-se também investigar a suscetibilidade de diferentes órgãos de reserva de distintas espécies botânicas à espécie bacteriana. Este trabalho mostra e confirma que outras espécies que não as de Pectobacterium spp. são capazes de incitar podridões-moles em órgãos de reserva.A soft rot inducing bacterium was isolated from decaying potato tubers and attempts were made to identify it at species level. Biochemical, biological and staining procedures allowed to identify it as belonging to the species Pseudomonas viridiflava. Additionally, the potential pathogenicity and host range of the identified isolate was checked by artificial inoculation of storage organs of different plants. This paper showed and confirmed that phytobacteria other than Pectobacterium spp are able to induce soft rot and that this fact deserves further investigation as far as crop losses due to prokaryotes are concerned.

  7. Efeito da fertilização fosfatada na culturada batata (solanum tuberosum l. no planalto do huambo (Angola Effects of phosphorus fertilization on potato (solanum tuberosum l. crop production, in huambo country (Angola

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    Imaculada da Conceição Henriques

    2010-12-01

    Full Text Available Relatam-se ensaios realizados, no ano agrícola 2004/5, em blocos completos casualizados, para estudo do efeito na produção de doses de fertilização fosfatada da cultivar de batata-semente importada ‘Romano’ e da cultivar regional ‘Tchigembo’, plantadas em duas datas (Outubro e Fevereiro da época das chuvas e numa terceira data (Junho, na época seca, praticada em regadio. Os ensaios foram repetidos em três locais da Província do Huambo - Bailundo, Chianga e Calenga - distanciados de cerca de 50 km. Avaliaram-se os efeitos, na produção total e comercial de tubérculos (diâmetro > 25 mm, de diferentes doses de fósforo, na forma de superfosfato simples (24% de P2O5, tendo-se aplicado uniformemente ureia (300 kg ha-1 de N, fraccionada em três aplicações. As doses de fósforo ensaiadas foram 0, 50, 100, 200, 400 e 800 kg ha-1 de P2O5, comparadas com uma testemunha sem qualquer aplicação de fertilizante, azotado ou fosfatado. As modalidades de fertilização azotada receberam, uniformemente 300 de azoto. A dose que maior benefício económico originou, sem desperdício de químicos para o meio ambiente, foi a de 200 kg ha-1 de P2O5.Several trials were carried out to compare total and commercial potato yield of the imported cultivar ‘Romano’ and the regional cultivar ‘Tchigembo’, planted in two dates on rain season (October and February and in a third date (June, in dry season, with irrigation. The trials were repeated in three locations of the Huambo country - Bailundo, Chianga and Calenga. The effects on total and commercial (tubers diameter > 25 mm potato yields of different amounts of phosphorus, applied with single superphosphate (24% of P2O5 were evaluated. Treatments were: control (any type of soil fertilization null, 50, 100, 200, 400, and 800 kg ha-1 of P2O5. Phosphorus treatments received also 300 kg ha-1 of nitrogen (urea applied uniformly, in three times. The greatest economic benefice for both potato

  8. Efeito do Solo do Materias Organicos E do Adubo Formula 4N:14P:8K Para Producao DA Batata (Solanum tuberosum L.) Semente Pre-Basica no Casa de Vegetacao

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    László, Márton

    2010-05-01

    hard effect (57%). Our results are shown that it was possible developing of the seed potato production under tropical greenhouse conditions by optimalised soil-organic matter-fertilizer system. This datas should be as indicators to sustainable field potato advisory systems. Keywords: potato (Solanum tuberosum L.), greenhouse, latossolo vermelho soil, farmyard manure, burnt rice straw, 4N:14P:8K fertilizer, sustainability, yield RESUMO A batata é atualmente uma das hortaliças de maior importância no Brasíl. Nos conduzirémos os três experimentos para aumentár-se do produção e produtividade da batata (Solanum tuberosum L.) semente pré- básica no casa de vegetação da Brazília-DF, no Empresa Brasileira de Pesquisa Agropecuaria- Centro Nacional de Pesquisas de Hortaliças no 1990. Os três experimentos (latossolo vermelho novo x esterco de curral x palha de arroz queimado, latossolo vermelho novo x adubo 4:14:8 NPK, latossolo vermelho novo x esterco de curral x palha de arroz queimado x adubo 4:14:8 NPK) no casa de vegetação foram conduzidos com total 29 combinações, no 5-5-3 repetições com total parcelas de 116. Os resultados foram submetidos a analise de variáncia, ANOVA e MANOVA. Nossos principal resultados estam apresentándo abaixo. 1. A mistura de 80% latossolo vermelho novo, 10% palha de arroz queimado e 10% de esterco de curral, apresentou os maiores valores para numero de tuberculos com 0-20 mm, peso total de tuberculos com 0-20 mm e peso total de tuberculos por vaso. 2. Há um efeito grande crescente das doses de 4N:14P:8K nos caracteres observados. 3. Analise-se do latossolo vermelho novo x esterco de curral x palha de arroz queimado x adubo 4:14:8 NPK experimento os resultados apresentárám-se que entre nas misturas também foi melhor a 80% latossolo vermelho novo, 10% palha de arroz queimado, 10% esterco de curral. Examinando-se 15 fatores, entre 11 casos afirmou-se a mistura como para melhor que a outra mistura. Nossos resultados

  9. Solanum Tuberosum Supplementation for Biogas Production

    OpenAIRE

    Pradip B. Acharya; Prateek Shilpkar

    2015-01-01

    Production of biogas using animal dung is well documented. Nutritional supplement enhances microbial activity and ultimately results in increase biogas production. Solanum tuberosum (potato) is a vegetable crop grown in most parts of world. It is a rich source of carbohydrate starch and many minerals. Present experiment was conducted in 5L capacity glass digester bottles filled with mixture of buffalo dung and water at 5.3% total solids. A total of six sets were prepared, three as control and...

  10. Natural diversity of potato (Solanum tuberosum) invertases

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    Li Li; Meller Sebastian; Draffehn Astrid M; Gebhardt Christiane

    2010-01-01

    Abstract Background Invertases are ubiquitous enzymes that irreversibly cleave sucrose into fructose and glucose. Plant invertases play important roles in carbohydrate metabolism, plant development, and biotic and abiotic stress responses. In potato (Solanum tuberosum), invertases are involved in 'cold-induced sweetening' of tubers, an adaptive response to cold stress, which negatively affects the quality of potato chips and French fries. Linkage and association studies have identified quanti...

  11. Microtuberization of potato (Solanum tuberosum L.)

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    Koleva Gudeva, Liljana; Trajkova, Fidanka; Stojkova, Irena

    2014-01-01

    This paper presents the results of the examination of the impact of phytohormon gibberellic acid GA3 on sprouts formation in in vivo conditions, and the impact of the phytohormones on induction of microtuberization under in vitro conditions in several varieties of seed tubers and mercantile potato tubers (Solanum tuberosum L.) Seed tubers potatoes tests were made with varieties Dido, Marabel, Agria, Ambition, and Agriko, while Agria SR, Agria BE and Andrea were the mercantile varieties of pot...

  12. Succession of crops in fitossanidade and crop productivity of potato (Solanum tuberosum L. Sucessão de culturas na fitossanidade e produtividade da cultura da batata (Solanum tuberosum L. Suceción de culturas na fitosanidad y productividad de la papa (Solanum tuberosum L.

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    Adenilsom dos Santos Lima

    2009-12-01

    Full Text Available The study was conducted in the experimental area of Department of Agronomy, campus Cedeteg of the State University Center West, Guarapuava - PR. It aimed to evaluate the productivity and classification of tubers and the severity and incidence of scab on the potato crop in succession crops of vetch (Vicia sativa L., oat (Avena strigosa L., wheat (Triticum aestivum L., radish (Raphanus sativus L. and fallow area. It was observed that the productivity and the diameter of the tubers were not affected by treatments. The cultivation of oat as predecessor increases the severity of common scab (Streptomyces spp, and fallow and culture of vetch as predecessor caused an increase in severity of silver scab (Helminthosporium solani Dur. e Mont..A pesquisa foi desenvolvida em área experimental do departamento de Agronomia no campus Cedeteg da Universidade Estadual do Centro Oeste, Guarapuava (PR. O objetivo foi avaliar a produtividade e a classificação dos tubérculos e a severidade e incidência de Sarna sobre a cultura da batata em sucessão às culturas da ervilhaca (Vicia sativa L., aveia preta (Avena strigosa L., trigo (Triticum aestivum L., nabo forrageiro (Raphanus sativus L. e área de pousio. Observou-se que a produtividade e o diâmetro dos tubérculos não foram afetados pelos tratamentos. O cultivo de aveia preta como antecessora aumenta a severidade da Sarna Comum (Streptomyces spp., e pousio e a cultura da ervilhaca como antecessor ocasionou aumento da severidade da Sarna Prateada (Helminthosporium solani Dur. e Mont..
    Pesquisa realizada em el compo experimental del Departamento de agronomia del Campus Cedeteg de la Universidad Estadual del Centro Oeste de Guarapuava (PR, Brasil. Su objetivo fue evaluar la productividad, clasificar los tubérculos y verificar la severidad de la ocurrencia de Sarna sobre

  13. Solanum Tuberosum Supplementation for Biogas Production

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    Pradip B. Acharya

    2015-04-01

    Full Text Available Production of biogas using animal dung is well documented. Nutritional supplement enhances microbial activity and ultimately results in increase biogas production. Solanum tuberosum (potato is a vegetable crop grown in most parts of world. It is a rich source of carbohydrate starch and many minerals. Present experiment was conducted in 5L capacity glass digester bottles filled with mixture of buffalo dung and water at 5.3% total solids. A total of six sets were prepared, three as control and three as test. Control sets were fed daily with buffalo dung water mixture throughout the period of experimentation, i.e. 80 days, whereas in test, from 51st day onward digesters were filled with mixture of dung and boiled potato with water. Feed was added daily in the amount of 120mL upto 80 days from beginning. On first day 12 mL fresh digested biogas slurry from running biogas plant was also added in all the digesters as inoculum. From 41th day onward biogas production was recorded by water displacement method and compared. Results reveal that in test digesters addition of potato shows an immediate and long lasting effect and increases biogas production between 90.48 and 192.86% higher than control sets.

  14. Natural diversity of potato (Solanum tuberosum invertases

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    Li Li

    2010-12-01

    Full Text Available Abstract Background Invertases are ubiquitous enzymes that irreversibly cleave sucrose into fructose and glucose. Plant invertases play important roles in carbohydrate metabolism, plant development, and biotic and abiotic stress responses. In potato (Solanum tuberosum, invertases are involved in 'cold-induced sweetening' of tubers, an adaptive response to cold stress, which negatively affects the quality of potato chips and French fries. Linkage and association studies have identified quantitative trait loci (QTL for tuber sugar content and chip quality that colocalize with three independent potato invertase loci, which together encode five invertase genes. The role of natural allelic variation of these genes in controlling the variation of tuber sugar content in different genotypes is unknown. Results For functional studies on natural variants of five potato invertase genes we cloned and sequenced 193 full-length cDNAs from six heterozygous individuals (three tetraploid and three diploid. Eleven, thirteen, ten, twelve and nine different cDNA alleles were obtained for the genes Pain-1, InvGE, InvGF, InvCD141 and InvCD111, respectively. Allelic cDNA sequences differed from each other by 4 to 9%, and most were genotype specific. Additional variation was identified by single nucleotide polymorphism (SNP analysis in an association-mapping population of 219 tetraploid individuals. Haplotype modeling revealed two to three major haplotypes besides a larger number of minor frequency haplotypes. cDNA alleles associated with chip quality, tuber starch content and starch yield were identified. Conclusions Very high natural allelic variation was uncovered in a set of five potato invertase genes. This variability is a consequence of the cultivated potato's reproductive biology. Some of the structural variation found might underlie functional variation that influences important agronomic traits such as tuber sugar content. The associations found between

  15. The enigma of Solanum maglia in the origin of the Chilean cultivated potato, Solanum tuberosum Chilotanum group

    Science.gov (United States)

    Landrace potato cultivars of Solanum tuberosum occur in two broad geographic regions; the high Andes from western Venezuela south to northern Argentina (S. tuberosum Andigenum Group), and lowland south central Chile (S. tuberosum Chilotanum Group). Chilotanum Group landraces are adapted to long days...

  16. Determinação de glicoalcalóides em batatas in natura (Solanum Tuberosum L. comercializadas na cidade de Campinas, Estado de São Paulo Determination of glycoalkaloides in potato tubers (Solanum Tuberosum L. marketed in the city of Campinas, State of São Paulo

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    Rita Margarete D. Machado

    2004-03-01

    Full Text Available A batata é uma das principais hortaliças cultivadas no Brasil, constituindo-se em um dos mais importantes alimentos na dieta humana, em decorrência de sua disponibilidade e características nutricionais. Entretanto, membros da família Solanaceae produzem, durante crescimento e após colheita, compostos potencialmente tóxicos denominados glicoalcalóides, dos quais alfa-solanina e alfa-chaconina predominam. A concentração máxima desses compostos em batata in natura considerada segura para consumo humano é estimada em 200mg·kg-1, expressa como glicoalcalóides totais (GAT. No presente estudo, foram extraídos e quantificados os GAT em amostras de tubérculos de batata in natura comercializados na cidade de Campinas, SP. A técnica utilizada foi a cromatografia líquida de alta eficiência (CLAE com coluna C18 e detetor de arranjo de diodos. O valor médio de recuperação foi 102,6% para alfa-solanina e 100,2% para alfa-chaconina. Os limites de quantificação foram 2,5µg·mL-1 para alfa-solanina e 1,4µg·mL-1 para alfa-chaconina. As concentrações de GAT em amostras individuais de tubérculos inteiros das diferentes variedades e tipos estudados (Bintje, Monaliza, Asterix e Bolinha variaram de 22,4 a 246,9mg·kg-1. Das amostras analisadas, 82% apresentaram níveis de GAT inferiores a 100mg·kg-1, o que indica que as variedades de batatas estudadas podem ser consideradas seguras para consumo humano.Potato is included among the main horticultural crops in Brazil. Due to its broad availability and nutritional characteristics, it has been considered one of the most important components in the human diet. Nevertheless, members of the family Solanaceae produce potentially toxic compounds known as glycoalkaloids during growth and post-harvest, amongst which alpha-solanine and alpha-chaconine predominate. The recommended safety level for these compounds in unprocessed potato tubers for human consumption, expressed as total glycoalkaloids, is

  17. COMPETIÇÃO ENTRE OITO VARIEDADES, GAÚCHAS E ALEMÃS DE BATATA COMPETITION BETWEEN EIGHT GAUCHA AND GERMAN POTATO (Solanum tuberosum L. VARIETIES IN GOIÂNIA-GO, BRAZIL

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    Lincoln F. Zica

    2007-09-01

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    As variedades de batata, Baronesa, Canguçu e Piratini, do Rio Grande do Sul e Achat, Echo, Fátima, Jetta e Porta, da Alemanha, foram comparadas quanto ao seu desenvolvimento e produção, no chamado plantio da seca, em Goiânia. Baronesa apresentou a maior produção total e de tubérculos grandes (acima de 60 g assim como maior número de tubérculos grandes, seguida pela Piratini. Ainda é necessário averiguar a aceitação dessas variedades na região porque seus tubérculos são de película rosada, enquanto o comércio local está acostumado a batatas amarelas. Das variedades alemãs, Porta e Fátima, embora apresentassem menor produção total e de tubérculos grandes, não diferiram estatisticamente das variedades gaúchas.

    Eight potato varieties, Baronesa, Canguçu and Piratini, from the state of Rio Grande do Sul, and the German varieties Achat, Echo, Fátima, Jetta and Porta were tested in this trial, during the dry season, in Goiânia, state of Goiás. Baronesa resulted the best one in total yield and production of large tubers (over 60 grams followed next by Piratini. However, there are doubts wether their pink skinned tubers would be accepted by the consumer of this region, accustomed to yellow potatoes. Among the German varieties, Porta and Fátima ranked best and even they yielded less than Baronesa and Piratini, there was no significant difference between the two groups.

  18. Serpentine leafminer (Liriomyza trifolii on potato (Solanum tuberosum: field observations and plant photosynthetic responses to injury Mosca-minadora (Liriomyza trifolii na cultura da batata (Solanum tuberosum: observações de campo e respostas fotossintéticas da planta à injúria

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    Adeney de Freitas Bueno

    2007-12-01

    Full Text Available Serpentine leafminers, Liriomyza spp. (Diptera: Agromyzidae, are polyphagous insects that feed on numerous crops worldwide including potato. Recently, leafminer larvae (Liriomyza trifolii have become an economically important pest of potato. The larvae eat the mesophyll of leaflets leaving long winding tunnels inside the leaflets. The photosynthetic effects of larval tunneling on the remaining leaf tissue are unknown. In 2003, physiological responses of potato to leafminer, L. trifolii were evaluated in Kearney, Nebraska, USA. The leaflets were examined 7 and 14 days post infestation for leaf area injury, photosynthetic rates and fluorescence. Leafminers caused up to 13% leaf area loss due to leafminer injury with no effect on the photosynthetic rates of the remaining leaf tissue thus having similar effects as other gross tissue removers. However, fluorescence measures revealed changes in the photosynthetic efficiency and depend of the type of injury, it may lead to early leaf senescence. Field monitoring of L. trifolii infestations showed that treatments with abamectin were effective in reducing leafminer numbers and had no immediate effect on beneficial parasitoid from Eulophidae family suggesting that abamectin is a good option for chemical control.Moscas-minadoras, Liriomyza spp. (Diptera: Agromyzidae, são pragas polífagas distribuídas por todo o mundo, que se alimentam de diferentes culturas, incluindo a da batata. Recentemente, a mosca-minadora (Liriomyza trifolii tem tornado-se uma praga importante na cultura da batata. As larvas comem o mesófilo foliar, deixando longas minas no interior das folhas. O efeito na fotossíntese do tecido foliar remanescente das minas é desconhecido. Em 2003, as respostas fisiológicas das plantas de batata à mosca-minadora L. trifolii foram avaliadas no condato de Kearney, Nebraska, EUA. As avaliações de área foliar danificada, fotossíntese e fluorescência foram feitas aos 7 e 14 dias após a

  19. OBSERVAÇÕES SOBRE DEGENERESCÊNCIA E COMPORTAMENTO EM DIFERENTES ÉPOCAS DE PLANTIO DE QUATRO VARIEDADES DE BATATA (Solanum Tuberosum L. COMMENTS ON DEGENERATION AND BEHAVIOR AT DIFFERENT PLANTING DATES OF FOUR POTATO (Solanum tuberosum L. VARIETIES

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    Peter E. Sonnenberg

    2007-09-01

    Full Text Available

    As variedades de batata DELTA-A, HANSA, HYDRA e TONDRA foram plantadas em duas gerações, a primeira na seca e a segunda, nas águas de 1971, em Goiânia. Para o primeiro plantio, usaram-se batatas-sementes importadas, para o segundo, as filhas destas. HANSA, HYDRA e TONDRA produziram mais na segunda geração do que na primeira, devido às melhores condições da época chuvosa. A produção da DELTA-A praticamente não variou nas duas épocas. HANSA foi significantemente superior às outras, em produção total, nas duas épocas e em produção de tubérculos comerciáveis (acima de 33 mm, na época chuvosa. Neste período, seus tubérculos grandes, (acima de 40 mm apresentaram-se com superdesenvolvimento de lenticelas e manchados internamente, ficando comercialmente desvalorizados. A variedade HYDRA destacou-se das demais pela produção de batatas grandes, principalmente na seca. Mostrou-se, porém, muito suscetível à sarna comum, cujo ataque inutilizou grande parte de seus tubérculos. TONDRA foi a variedade que melhor se adaptou ao período chuvoso. Produziu duas vezes mais tubérculos comerciáveis e quase três vezes mais tubérculos grandes, que na seca.

    The four potato varieties DELTA-A, HANSA, HYDRA and TONDRA were tested in the dry and in the rainy season of 1971, in Goiânia. Imported certified seed potatoes were used for the first planting and the harvested tubers, for the second. All varieties, except DELTA-A, yielded more in the rainy than in the dry season. This difference was found to be caused by the better water supply during the rainy season. There was no significant influence of the season on the production of DELTA-A. HANSA produced in both seasons significantly more in total weight than the other varieties and more marketable potatoes (over 33 mm in the rainy season. But the large

  20. Histo-anatomy of the stem of Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Monica SIPOS

    2008-05-01

    Full Text Available The paper reveals the anatomical structure of the stem of Solanum tuberosum L. in the incipient stages of the ontogenetic development (the popular denomination „sprout”. Cross sections of the stem of this species were performed. The appearance of the secondary structure – even in this early ontogenetic stage – was confirmed by the presence of the cambium and the tissues generated by this. As a result, at the potato, the purely primary structure of the stem is of a short duration.

  1. Expressão eficiente do gene reporter beta-glucuronidase nos tecidos vasculares de batata (Solanum tuberosum L. utilizando de um promotor específico (BRA3 de Agrobacterium rhizogenes Efficient expression of beta-glucuronidase reporter gene in vascular tissue of potato (Solanum tuberosum L. utilizing a specific promoter (BRA3 from Agrobacterium rhizogenes

    Directory of Open Access Journals (Sweden)

    Antonio Carlos Torres

    2003-06-01

    Full Text Available Promotores tecido-específico controlam a transcrição de genes em diferentes tecidos vegetais bem como em diferentes estádios de desenvolvimento da planta, levando à indução de distintos níveis de atividade transiente e/ou estável do gene. Tais promotores podem ser empregados para a expressão seletiva de genes de interesse. O promotor rol A de Agrobacterium rhizogenes, por exemplo, é floema-específico, sugerindo que possa ser empregado em estratégias de defesa de plantas que são infectadas por vírus com replicação restrita ao floema. A expressão do gene marcador da ß-glucuronidase (gus dirigido pelo promotor rol A (pBRA3 foi observada em plantas transgênicas de batata (cvs. Macaca e Baronesa. Entrenós e secções de folhas foram submetidos ao cocultivo com A. tumefaciens. A atividade do gene gus avaliada em brotações resistentes à canamicina não se restringiu ao floema (alto nível de expressão do gene, mas também se manifestou no xilema dos caules. As expressões transiente e estável são, no entanto, tecido-específicas, localizadas sobretudo no sistema vascular de entrenós e ausente em raízes e folhas. As plantas gus positivas foram micropropagadas, plantadas em casa de vegetação e avaliadas por PCR, utilizando-se 'primers' específicos para o gene npt II. Nenhuma alteração fenotípica foi observada em plantas transgênicas, em relação às não transformadas.Tissue-especific promoters allow the modulation of gene transcription in different tissue types as well as in different stages of plant development, leading different levels of transient and stable activity of the gene product. These promoters have been employed for selective gene expression. The Agrobacterium rhizogenes rol A gene promoter (BRA3 controls phloem-specific expression indicating that this promoter might have an important role in plant defense strategies against virus which replicated only in the phloem. The expression of

  2. Organic and chemical fertilizers application effects on potato production soil's fertility (Solanum tuberosum)

    OpenAIRE

    Herrera, E A

    2007-01-01

    Objectives, treatments, activities and preliminary results of the project that evaluates the effects of organic and chemical fertilizers application on potato production soil's fertility (Solanum tuberosum). available in SANREM office, ESIILTRA-4 (Practices and Strategies for Vulnerable Agro-Ecosystems)

  3. Pollen viability and meiotic analysis of Solanum commersonii commersonii Dun., Solanum commersonii malmeanum Bitt. and Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Alexandre Alonso Alves

    2007-01-01

    Full Text Available Meiotic abnormalities in potato hamper sexual recombination, due to their influence on pollen production andviability rate. In this study we evaluated pollen viability and meiosis of three clones of Solanum commersonii commersoniiDun. (SCC, two of Solanum commersonii malmeanum Bitt. (SCM and seven clones and four cultivars of Solanum tuberosumL., with the purpose of indicating promising genotypes for genetic breeding of potato. Early chromosome migration atmetaphases I and II and chromosome pairing anomalies were the main causes of pollen inviability in the evaluated genotypes.Clones SCC 07 and SCM 60 are the most suitable for sexual recombination, owing to the high percentage of viable pollengrains and low frequencies of meiotic abnormalities.

  4. Effect of ozone on indicators of leaf aging. [Solanum tuberosum

    Energy Technology Data Exchange (ETDEWEB)

    Yisheng Ni; Yaoren Dai; Fayek Negm; Reddy, N. Flores, H.; Arteca, R.; Pell, E. (Pennsylvania State Univ., University Park (United States))

    1991-05-01

    Ozone (O{sub 3}) stress induces accelerated foliar senescence, as measured by a decline in ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco). The authors wish to determine (1) whether the decline in rubisco is under genetic regulation and (2) what role is played by ethylene and polyamines in modulating this response. Plants of Solanum tuberosum L. cv. Norland were grown in a charcoal filtered greenhouse and treated with 0.15 {mu}1 1{sup {minus}1} O{sub 3} in continuous stirred tank reactors for 4 h. Immediately, 4 h and 20 h after O{sub 3} exposure, the fourth, seventh and tenth leaves from the apex were harvested and the tissue analyzed for ethylene emission, ethylene forming enzyme, malonyl transferase, ACC, malonyl ACC, spermine, spermidine, putrescine, ornithine decarboxylase and messenger RNA for the large and small subunits of rubisco. Ozone induced changes in most of the variables studied and the response was most evident in the younger tissue. In contrast O{sub 3} induces accelerated senescence of the oldest tissue. The relationship between O{sub 3}-induced changes in younger foliage and accelerated aging of older tissue will be the subject of further investigation.

  5. Antibacterial effects of Solanum tuberosum peel ethanol extract in vitro

    Directory of Open Access Journals (Sweden)

    Amanpour Raana

    2015-04-01

    Full Text Available Introduction: Today, medicinal plants are being widely used due to being natural, available, and cheaper than synthetic drugs and having minimum side effects. Since there were reports about the antibacterial properties of Solanum tuberosum (SE, the aim of this study was to investigate the antibacterial effects of SE ethanol extract in vitro condition on Streptococcus pyogenes, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae. Methods: Ethanol extract of SE peel was prepared by maceration method. Initially, antibacterial activity of ethanol extract of SE was qualitatively determined by disk diffusion test; then, the minimum inhibitory concentration and minimum bactericidal concentration were qualitatively determined by micro-dilution method. Results: SE peel extract had antibacterial properties and its effect was more pronounced on gram-positive bacteria, especially S. aureus (0.62±0.00 mg/ml. The extract had antibacterial activity on gram-negative bacteria, P. aeruginosa, too (8.33±2.88 mg/ml. Conclusion: SE peel extract has antibacterial activity and its effect on gram-positive bacteria was more pronounced than the investigated gram-negative bacteria. Therefore, it is suggested that SE peel constituent compounds be determined and to determine the exact mechanism of its antibacterial properties, and more comprehensive research be done to apply it, clinically.

  6. Effect of wounding and light exposure on sterol, glycoalkaloid, and calystegine levels in potato plants (Solanum tuberosum L. group Tuberosum)

    OpenAIRE

    Arif, Usman

    2013-01-01

    Steroidal glycoalkaloids (SGA) are neurotoxic substances that are present in some members of the Solanaceae family, including crop species like potato (Solanum tuberosum L.) and tomato. The SGA level in the potato tuber is a genetic trait, but certain environmental factors such as wounding and light exposure can increase SGA levels several-fold, which may render tubers unsuitable for human consumption. There is little information about SGA biosynthesis. The sterol cholesterol is commonly rega...

  7. Pseudogamic production of dihaploids and monoploids in Solanum tuberosum and some related species

    NARCIS (Netherlands)

    Breukelen, van E.W.M.

    1981-01-01

    Attempts were made to maximize frequencies of dihaploids from Solanum tuberosum, obtained through pseudogamy after pollination with S.phureja. Factors influencing dihaploid frequencies were studied: genetics of the pollinator effect, genetics of the seed parent effect and interaction between the two

  8. Potato (Solanum tuberosum) greenhouse tuber production as an assay for asexual reproduction effects from herbicides

    Science.gov (United States)

    The present study determined whether young potato plants can be used as an assay to indicate potential effects of pesticides on asexual reproduction. Solanum tuberosum (Russet Burbank) plants were grown from seed pieces in a mineral soil in pots under greenhouse conditions. Plant...

  9. Partial root zone drying (PRD) sustains yield of potatoes (Solanum tuberosum L.) at reduced water supply

    DEFF Research Database (Denmark)

    Shahnazari, Ali; Andersen, Mathias Neumann; Liu, Fulai;

    2008-01-01

    Partial root zone drying (PRD) is a new water-saving irrigation strategy being tested in many crop species. Until now it has not been investigated in potatoes (Solanum tuberosum L.). A field experiment on sandy soil in Denmark was conducted under a mobile rainout shelter to study effects of two...

  10. Assignment of genetic linkage maps to diploid Solanum tuberosum pachytene chromosomes by BAC-FISH technology

    NARCIS (Netherlands)

    Tang, X.; Boer, de J.M.; Eck, van H.J.; Bachem, C.W.B.; Visser, R.G.F.; Jong, de J.H.

    2009-01-01

    A cytogenetic map has been developed for diploid potato (Solanum tuberosum), in which the arms of the 12 potato bivalents can be identified in pachytene complements using multicolor fluorescence in situ hybridization (FISH) with a set of 60 genetically anchored bacterial artificial chromosome (BAC)

  11. Stem-end defect in chipping potatoes (Solanum tuberosum L.) as influenced by mild environmental stresses

    Science.gov (United States)

    Global consumption of potato (Solanum tuberosum, L.) continues to shift from fresh potatoes to value-added processed food products such as potato chips. One serious tuber quality defect of chipping potatoes is stem-end chip defect, which results in chips with dark vasculature and adjacent tissues at...

  12. GENE-REGULATION IN INTERTYPIC HETEROKARYONS OF SOLANUM-TUBEROSUM AND NICOTIANA-TABACUM TISSUE PROTOPLASTS

    NARCIS (Netherlands)

    VANKESTEREN, WJP; BIJMOLT, EW; TEMPELAAR, MJ

    1994-01-01

    Activities of the beta-glucuronidase (GUS) reporter enzyme were evaluated in transgenic plants, protoplasts, and intertypic heterokaryons of Solanum tuberosum and Nicotiana tabacum. With GUS under control of the promoter of the cauliflower-mosaicvirus 35S RNA gene (CaMV), activities of the enzyme we

  13. Farmyard Manure and Fertilizer Effects on Seed Potato (Solanum tuberosum L.) Yield in Green House Production

    Science.gov (United States)

    László, M.

    2009-04-01

    fertilizer (18.0 g pot-1) with a hard effect (57%). Our results are shown that it was possible developing of the seed potato production under tropical greenhouse conditions by optimalised soil-organic matter-fertilizer system. This datas should be as indicators to sustainable field potato advisory systems. Keywords: potato (Solanum tuberosum L.), greenhouse, latossolo vermelho soil, farmyard manure, burnt rice straw, 4N:14P:8K fertilizer, sustainability, yield Introducáo: Importância e situação actual em produção da batata no Brazíl A batata é atualmente uma das hortaliças de maior importância no Brasíl (Márton 2000a., 2000b.) com um cultivo annual médio de 173.000 ha e uma produção de 2600000 t. A produtividade médio nacional é de 15 t ha-1, muito baixa se considerar que é possivel a obtenção de rendimentos acima de 40 t ha-1. Observa- se também, que existe variação no produtividade entre regiões e estados. E importante como fonte de alimento pelo seu alto valor nutricional a quantidade produzida muito superior por unidade de área a tempo, se comparada com diversas outras culturas (László 2000b., 2000c.). Os estados que tradicionalmente produzem batata em maior ou menor escala são indicados em seguente: Pernambuco, Ceará, Sergipe, Goiás, Mato Grosso, Mato Grosso do Sul, Rondonia e Acre. Os plantios predominantes são o das águas e das secas, sendo o de inverno bem menos expressivo, pois poucas áreas permitem o seu cultivo, na maioria dos casos necessitando- se de irrigação. Considerando as três épocas de plantio e diferentes condições climaticas brasileiras, podemos definir de um modo geral o plantio de batata no Brasíl da seguinte maneira: Nordeste e Centro- Oeste- plantio de inverno, Sudeste- plantio das águas, secas e inverno, Sul- plantio das aguas, secas e inverno. Sendo este último em áreas muito limitadas. Dentre as hortaliças a batata é uma das culturas mais estudadas actualmente. Os principais problemas que afetam a

  14. Avaliação da infestação de insetos-praga associados à batata (Solanum tuberosum L. sob efeito de nutrientes nitrogenados e potássicos e teores acumulados de aminoácidos livres nas cultivares Achat e Monalisa

    Directory of Open Access Journals (Sweden)

    Edson Henrique de Azeredo

    2002-01-01

    Full Text Available Evaluation of insect-pest infestation associated to potato (Solanum tuberosum L. under effect of nitrogen and potassium fertilizers and the accumulated amount of free aminoacids in Achat and Monalisa cultivars. The objective of this work was to evaluate the occurence of insect-pests on potato plants influenced by dosages of nitrogen and potassium accumulated in plant organs. A total of 169 plants of the Achat and Monalisa cultivars were evaluated to determine the presence-absence of Diabrotica speciosa Germar, 1824 and Agrotis ipsilon Hüfnagel, 1767. The experiment was carried out and executed at the Universidade Federal Fluminense, and the delineation was complete randomized block design, with four replication and nine treatments, using three fertilization level (0; 75 and 150 Kg/ha with N-urea + KCl. The aminoacid levels were adjusted by the Leucine standard-curve (µg/l, using the Ninhydrin method, at 570 nm. The results showed that the tubercles of Monalisa accumulated high free aminoacid levels with 7,95% in the treatment N1K2 and 7,75% in the N2K1.These treatments, induced the infestation by D. speciosa larvae in 27,03%, when the aminoacid level was 2,01 ± 0,58% (X ± EP, with probability of 0,0196

  15. Impacto dos nutrientes N e K e de açúcares solúveis sobre populações de Diabrotica speciosa (Germar (Coleoptera, Chrysomelidae e Agrotis ipsilon (Hüfnagel (Lepidoptera, Noctuidae na cultura da batata, Solanum tuberosum L. (Solanaceae Impact of the nutrients N and K and soluble sugars on Diabrotica speciosa (Germar (Coleoptera, Chrysomelidae and Agrotis ipsilon (Hüfnagel (Lepidoptera, Noctuidae populations in potato crops, Solanum tuberosum L. (Solanaceae

    Directory of Open Access Journals (Sweden)

    Edson Henrique de Azeredo

    2004-03-01

    Full Text Available Foi estudada a ocorrência de Diabrotica speciosa (Germar, 1824 (Coleoptera, Chrysomelidae e de Agrotis ipsilon (Hüfnagel, 1767 (Lepidoptera, Noctuidae em plantas de batata, cultivares Achat e Monalisa, influenciadas por dosagens de nitrogênio e potássio, e teor mínimo de açúcares solúveis. Os seguintes parâmetros foram avaliados: concentração de nutrientes minerais e açúcar em folha verde, folha senescente, folha em abcisão, haste, tubérculo e planta total usando extratos de infusão em etanol 80%. A maior infestação por larvas de D. speciosa foi na cultivar Monalisa a 150 kg.ha-1 de N + K com 27,03% a PThe occurrence of Diabrotica speciosa (Germar, 1824 and Agrotis ipsilon (Hüfnagel, 1767 on the potato cultivars Achat and Monalisa, influenced by nitrogen and potassium dosage, and minimum theor of soluble sugars, was studied. The following parameters were evaluated: concentration of mineral nutrient and sugar in green leaf, senescent leaf, leaf in abscission, stem, tubercle and total plant using extracts of infusion in ethanol 80%. The largest infestation of D. speciosa larvae was on Monalisa cultivar at 150 kg.ha-1 of N + K with 27.03% at P< 0,05. It was observed that the effect of the dosage of N + K in the increment of the concentration of soluble sugars increased the damages in the tubercles and stems by A. ipsilon. The infestation by these species increased to 58.82% on the Monalisa cultivar, when the nitrogen dosage increased from zero to 150 kg.ha-1, in the absence of potassium. On the other hand, high dosage of K reduced the damages by A. ipsilon on Monalisa cultivar. However, it did not influence the storage of soluble sugar. The results indicated that in Achat cultivar the accumulated soluble sugar was reduced, probably sensibilized by elevation of potassic fertilization dosing, differing from Monalisa cultivar, in which the influence was by nitrogen dosing.

  16. Local and systemic responses induced by aphids in Solanum tuberosum plants

    OpenAIRE

    Dugravot, S.; Brunissen, L.; Létocart, E.; Tjallingii, W.F.; Vincent, C; Giordanengo, Ph.; Cherqui, A.

    2007-01-01

    The aphids Macrosiphum euphorbiae (Thomas) and Myzus persicae (Sulzer) (Homoptera: Aphididae) are serious pests of potato (Solanum tuberosum L.) (Solanaceae), notably in transmitting several plant viruses. Heterospecific interactions may occur between these two species as they are often seen at the same time on the same potato plant in the field. As aphid infestation is known to induce both local and systemic changes, we conducted experiments to determine the effect of previous infestation on...

  17. Comparative genomics of the bacteria Dickeya solani and Pectobacterium wasabiae,emerging pathogens of Solanum tuberosum

    OpenAIRE

    Khayi, Slimane

    2015-01-01

    The pectolytic bacteria Pectobacterium and Dickeya species cause important diseases on Solanum tuberosum and other arable and horticultural crops. These bacteria are responsible for blackleg in the field and tuber soft rots in storage and in transit as well as in the field worldwide. The main objectives of this thesis are: 1) To study the diversity of a D. solani population using comparative genomics approaches in order to understand the genomic structure and evolution of this emerging specie...

  18. Large Scale Magnetic Separation of Solanum tuberosum Tuber Lectin from Potato Starch Waste Water

    Science.gov (United States)

    Safarik, Ivo; Horska, Katerina; Martinez, Lluis M.; Safarikova, Mirka

    2010-12-01

    A simple procedure for large scale isolation of Solanum tuberosum tuber lectin from potato starch industry waste water has been developed. The procedure employed magnetic chitosan microparticles as an affinity adsorbent. Magnetic separation was performed in a flow-through magnetic separation system. The adsorbed lectin was eluted with glycine/HCl buffer, pH 2.2. The specific activity of separated lectin increased approximately 27 times during the isolation process.

  19. Assessment of the processing profile of six “creole potato” genotypes (solanum tuberosum phureja group)

    OpenAIRE

    2012-01-01

    Six diploid potato (“creole potato”, Solanum tuberosum PhurejaGroup) genotypes were grown at the localities of Soacha, Duitama and Mosquera (Colombia) and were assessed for their suitability for industrial processing using Individual Quick Freezing (IQF) as pickled and dehydrated (flakes) potatoes. The following variables were assessed: Percentage of defective tubers, dry weight, specific gravity, “eye” depth, skin type, shape, appearance, color, aroma, flavor and texture. Cultivar Criolla Co...

  20. Thermal control of some post-harvest rot pathogens of Irish potato (solanum tuberosum l.)

    OpenAIRE

    Salami Olusola Abiodun; Popoola Omololu Olumide

    2007-01-01

    Thermal control effect on the incidence of some post-harvest rot pathogens of Solanum tuberosum (potato) was investigated in this study. Three cultivars of potato tuber whose local names are, Patiska, Mai Bawondoya and Nicola were used for the study. Five pathogenic fungi viz: Botryodiplodia theobromae, Fusarium redolens, Fusarium oxysporum, Penicillium sp. and Rhizopus oryzae associated with post harvest storage rot of root-tubers, were isolated from diseased potatoes. Among the three specie...

  1. Evaluation of Soil and Plant Nitrogen Tests in Potato (Solanum tuberosum L.) Production

    OpenAIRE

    Boris Lazarević; Milan Poljak; Tomislav Ćosić; Tea Horvat; Tomislav Karažija

    2014-01-01

    Nitrogen (N) management is critical in optimizing potato (Solanum tuberosum L.) yield and reducing environmental pollution. Several plant and soil based tests were proposed for assessment of N status in crop plants. Aim of this study was to evaluate the convenience of plant based tests (leaf chlorophyll content index (CCI) and petiole nitrate-nitrogen (NO3-N) concentrations) and soil based test (soil nitrogen (Nmin) content) for estimating potato N status. Experiment was conducted in N...

  2. Evaluation of antifungal activity of protease inhibitors from potato (Solanum tuberosum L.)

    OpenAIRE

    REISEROVÁ, Jana

    2014-01-01

    This diploma thesis is concerned on protease inhibitors isolated from potato (Solanum tuberosum L.) tubers and evaluation of their antifungal properties. Theoretical part of the thesis deals with protease inhibitors which have an antifungal effect. Tubers of potato cultivars Adéla, Ornella, Eurostarch - were used for protease inhibitors isolation. Antifungal activity of isolated protein fractions were evaluated versus fungi from genus Rhizoctonia and Fusarium that are important pathogens in a...

  3. Population Dynamics of Soil Pseudomonads in the Rhizosphere of Potato (Solanum tuberosum L.)

    OpenAIRE

    Loper, Joyce E.; Haack, Caryn; Schroth, Milton N.

    1985-01-01

    Rhizosphere population dynamics of seven Pseudomonas fluorescens and Pseudomonas putida strains isolated from rhizospheres of various agricultural plants were studied on potato (Solanum tuberosum L.) in field soil under controlled environmental conditions. Rhizosphere populations of two strains (B10 and B4) were quantitatively related to initial seed piece inoculum levels when plants were grown at −0.3 bar matric potential. At a given inoculum level, rhizosphere populations of strain B4 were ...

  4. The systemin precursor gene regulates both defensive and developmental genes in Solanum tuberosum

    OpenAIRE

    Narváez-Vásquez, Javier; Ryan, Clarence A.

    2002-01-01

    Transformation of Solanum tuberosum, cv. Desiree, with the tomato prosystemin gene, regulated by the 35S cauliflower mosaic virus promoter, resulted in constitutive increase in defensive proteins in potato leaves, similar to its effects in tomato plants, but also resulted in a dramatic increase in storage protein levels in potato tubers. Tubers from selected transformed lines contained 4- to 5-fold increases in proteinase inhibitor I and II proteins, >50% more soluble and dry weight protein, ...

  5. Comparison of the calystegine composition and content of potato sprouts and tubers from Solanum tuberosum Group Phureja and Solanum tuberosum Group Tuberosum.

    Science.gov (United States)

    Griffiths, D Wynne; Shepherd, Tom; Stewart, Derek

    2008-07-01

    The calystegines detected in tubers from 17 Phureja ( S. tuberosum Group Phureja) lines and five Tuberosum ( S. tuberosum Group Tuberosum) cultivars were identified as the A 3 and B 2 structural types. Their concentration in whole tubers was of a similar order of magnitude in both species, as was the variability in the ratio of B 2 to A 3. On average, calystegine concentrations in the peel were about 13 times that found in the flesh for the five Tuberosum cultivars, and 4 times higher for four Phureja lines. Removal of the peel reduced the calystegine content by an average of over 50% in Tuberosum but by only 30% in Phureja, despite the latter having the greater proportion of peel. The calystegine content of sprouts was also determined for five Tuberosum cultivars and four Phureja lines and was found to include small amounts of four additional types, B 3, B 4, N 1, and X 2, in addition to the more abundant A 3 and B 2. Concentrations in the sprouts of Tuberosum were on average 100 times higher than that in the tuber flesh and 8 times higher than in the peel, whereas for Phureja, the equivalent values were 30 and 7 times higher, respectively. No correlation was found between sprout concentration and either flesh or peel calystegine concentration. PMID:18533664

  6. Bioinformatic identification of microRNAs and their target genes from Solanum tuberosum expressed sequence tags

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    MicroRNAs (miRNAs) are a class of non-coding RNAs that regulate gene post-transcriptional expression in plants and animals. Low levels of some miRNAs and time- and tissue-specific expression patterns lead to the difficulty for experimental identification of miRNAs. Here we present a bioinformatic approach for expressed sequence tags (ESTs) prediction of novel miRNAs as well as their targets in Solanum tuberosum. We blasted the databases of S. Tuberosum ESTs to search for potential miRNAs, using previously known miRNA sequences from Arabidopsis, rice and other plant species. By analyzing parameters of plant precursors, including secondary structure, stem length and conservation of miRNAs, and following a variety of filtering criteria, a total of 22 potential miRNAs were detected. Using the newly identified miRNA sequences, we were able to further blast the S. Tuberosum mRNA database and detected 75 potential targets of miRNAs in S. Tuberosum. According to the mRNA annotations provided by the National Center for Biotechnology Information (NCBI) (http://www.ncbi.nlm.nih.gov/), most of the miRNA target genes were predicted to encode transcription factors that regulate cell growth and development, signaling, and metabolism.

  7. The Transcriptome of Compatible and Incompatible Interactions of Potato (Solanum tuberosum) with Phytophthora infestans Revealed by DeepSAGE Analysis

    DEFF Research Database (Denmark)

    Gyetvai, Gabor; Sønderkær, Mads; Göbel, Ulrike;

    2012-01-01

    Late blight, caused by the oomycete Phytophthora infestans, is the most important disease of potato (Solanum tuberosum). Understanding the molecular basis of resistance and susceptibility to late blight is therefore highly relevant for developing resistant cultivars, either by marker-assissted s......Late blight, caused by the oomycete Phytophthora infestans, is the most important disease of potato (Solanum tuberosum). Understanding the molecular basis of resistance and susceptibility to late blight is therefore highly relevant for developing resistant cultivars, either by marker...

  8. Light Spectral Quality Effects on the Growth of Potato (Solanum Tuberosum L.) Nodal Cutttings in Vitro

    Science.gov (United States)

    Wilson, Deborah A.; Weigel, Russell, C.; Wheeler, Raymond M.; Sager, John C.

    1993-01-01

    The effects of light spectral quality on the growth of in vitro nodal cutting of potato (Solanum tuberosum) cultivars Norland, Superior, Kennebec, and Denali were examined. The different light spectra were provided by Vita-Lite fluorescent (VF) (a white light control), blue fluorescent (BF), red fluorescent (RF), low-pressure sodium (LPS), and a combination of low-pressure sodium plus cool-white fluorescent lamp (LPS/CWF). Results suggested that shoot morphologic development of in vitro grown potato plants can be controlled by controlling irradiant spectral quality.

  9. Large Scale Magnetic Separation of Solanum tuberosum Tuber Lectin from Potato Starch Waste Water

    Czech Academy of Sciences Publication Activity Database

    Šafařík, Ivo; Horská, Kateřina; Martinez, L. M.; Šafaříková, Miroslava

    Melville : American institute of physics, 2010 - (Häfeli, U.; Schütt, W.; Zborowski, M.), s. 146-151 ISBN 978-0-7354-0866-1. ISSN 0094-243X. - (AIP Conference Proceedings. 1311). [International Conference on the Scientific and Clinical Applications of Magnetic Carriers /8./. Rostock (DE), 25.05.2010-29.05.2010] Institutional research plan: CEZ:AV0Z60870520 Keywords : drug delivery * magnetic microspheres and ferrofluids * magnetic nanospheres * molecular biology * stem cell separation * starch production * potato waste water * magnetic separation * chitosan * lectin * Solanum tuberosum Subject RIV: EH - Ecology, Behaviour

  10. ANÁLISIS DE MARCADORES MORFOLÓGICOS Y MOLECULARES EN PAPA (Solanum tuberosum L.)

    OpenAIRE

    Rose Onamu; Juan P. Legaria Solano; Jaime Sahagún Castellanos; José L. Rodríguez de la O; Joel Pérez Nieto

    2012-01-01

    La información sobre identificación, diversidad genética y relaciones entre genotipos de papa (Solanum tuberosum L.) es de importancia para su conservación eficiente, mejoramiento genético y utilización de los recursos genéticos de esta especie. Por tanto, con el objetivo de evaluar la eficiencia de los caracteres morfológicos y marcadores tipo RAPD e ISSR para discriminar genotipos de papa, se caracterizaron 15 variedades de papa cultivadas en México. Se evaluaron 12 caracteres cuantitativo...

  11. Differential Proteomic Analysis of Solanum tuberosum L. Leaves under Drought Stress

    Institute of Scientific and Technical Information of China (English)

    Yuan Su; Ping Yu; Xiaogan Zhou

    2012-01-01

    The research of molecular mechanism with anti-drought in Solanum tuberosum L.is important for breed improvement in potato to avoid yield loss caused by water deficit.Differential proteomics analysis of potato (anti-drought cultivar) leaves under drought stress were carried out using two-dimensional gel electrophoresis.42 differential expression protein spots were analyzed through gel map and identified by MALDI-TOF-TOF/MS.The main function of these proteins were stimulation response,cell development,metabolic and transport adjustment.The experiment can supply theory evidence to explain the anti-drought mechanism of anti-drought potato cultivar with multi-pathways.

  12. An Improved Method of Optimizing the Extraction of Polyphenol Oxidase from Potato (Solanum tuberosum L.) Peel

    OpenAIRE

    Mukherjee, Suprabhat; Bidyut BANDYOPADHAYAY; Bikram BASAK; Nilrudra MANDAL; Dey, Apurba; Mondal, Biswanath

    2012-01-01

    The present study has an objective to optimize the extraction of Polyphenol Oxidase (PPO) from potato (Solanum tuberosum L.) peel. Response surface methodology (RSM) was used to design experiments and study the effect of six influential extraction parameters: extraction buffer concentration (100-500 mM), pH of extraction buffer (4.5-8.5), time (1-12 hours), temperature (4-40�C), concentration of PMSF (1-5 mM) and volume of extraction buffer (200-1000 ml) on the extraction of PPO. The dependen...

  13. Respons Pemberian Coumarin Terhadap Produksi Mikro Tuber Planlet Kentang (Solanum tuberosum L.) Varietas Granola

    OpenAIRE

    Hasni, Vivi Ulfia

    2014-01-01

    The aim of this experiment is to determine the response to the administration of coumarin in micro-tuber production of potato plantlets (Solanum tuberosum L.) varieties of granola. The research was conducted at Tissue Culture Laboratory Experiment Berastagi at ± 1340 m above sea level, starting from October 2013 until February 2014 using a completely randomized factorial design with two factors, that is concentrations of coumarin (control; 0,025; 0,050; and 0,075 gram/l) and the volume of cou...

  14. FLUORIDE TOXICITY EFFECTS IN POTATO PLANT (SOLANUM TUBEROSUM L. GROWN IN CONTAMINATED SOILS

    Directory of Open Access Journals (Sweden)

    Chittaranjan Das

    2015-06-01

    Full Text Available This study aims to check the tolerance potential of Solanum tuberosum to accumulate fluoride (F. For this work S. tuberosum were grown for 87 days under five different concentrations of F viz. control, 11.05(T1, 22.11(T2, 44.21(T3, 110.53(T4 and 221.05(T5 mg per Kg NaF. Study results revealed that maximum reduction of root biomass (82.5 % at the fluoride dose 95 mg NaF/Kg soil. However, growth ratio and tolerance index showed opposite trend with concentration of F. On the other hand, F accumulation pattern was recorded highest in leaves and % of total F translocation from soil to plant linearly decreases with increasing added fluoride in soil. The F accumulation in leaves, root, shoot and potato tuber is 3.96 mg NaF per Kg, 3.02 mg NaF per Kg, 2.8 mg NaF per Kg and 1.56 mg NaF per kg, respectively. It was inferred from this study that potato (S. tuberosum accumulates fluoride at tissues level.

  15. Evidence from Solanum tuberosum in support of the dual-pathway hypothesis of aromatic biosynthesis

    Energy Technology Data Exchange (ETDEWEB)

    Morris, P.F.; Doong, R.L.; Jensen, R.A. (Univ. of Florida, Gainesville (USA))

    1989-01-01

    Key branchpoint enzymes of aromatic amino acid biosynthesis, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DS) and chorismate mutase (CM), have previously been shown to exist as separate compartmentalized isozymes in the chloroplasts and cytosol of tobacco, sorghum and spinach. Although additional examples of plants containing these isozyme pairs are accumulating, some studies in the literature report the presence of only the single plastidic DS or CM enzyme. Such apparent exceptions contradict the universality of pathway organization existing in higher plants that is implied by the dual-pathway hypothesis of aromatic biosynthesis. Since potato (Solanum tuberosum) exemplifies a case where only a single species of both DS and CM have been reported, we selected this system for further analysis. The DS-Mn and DS-Co isozyme pair, exhibiting all of the differential properties described in Nicotiana silvestris, have now been identified in S. tuberosum. Likwise, partial purification via DEAE-cellulose chromatography revealed two isozymes of CM in disks excised from tubers of S. tuberosum. The differential regulatory properties of these isozymes were comparable to the CM-1 and CM-2 isozymes of N. silvestris.

  16. Structure of two solanum tuberosum steroidal glycoalkaloid glycosyltransferase genes and expression of their promoters in transgenic potatoes

    Science.gov (United States)

    The Sgt2 gene in potato encodes a solanidine glucosyltransferase and is present as two distinct alleles expressed in cultivated potatoes. Promoter regions upstream from both steroidal glycoalkaloid biosynthetic gene alleles, Sgt2.1 and Sgt2.2, were isolated from Solanum tuberosum cv. Russet Burbank ...

  17. Measurement and modelling of ABA signalling in potato (Solanum tuberosum L.) during partial root-zone drying

    DEFF Research Database (Denmark)

    Liu, Fulai; Song, Ri; Zhang, Xiaoyan;

    2008-01-01

    The objective of this study was to develop a simple mechanistic model to predict the magnitude of ABA signalling ([X-ABA]) of potatoes (Solanum tuberosum L.) exposed to partial root-zone drying (PRD). Potatoes were grown in pots in a glasshouse with the roots split equally between two soil columns...

  18. Contribution to Solanum tuberosum L. tubergenesis, vitrocultivated under ultrabright color L.E.D.

    Directory of Open Access Journals (Sweden)

    Liviu POP

    2011-11-01

    Full Text Available This experiment had the main purpose to reduce the consumption of electric energy used in biotechnological vitroculture processes, in order to obtain cheaper seedling and keep the environment cleaner. To achieve this goal, we replaced CFLs with ultrabright LEDs, and, as biologic experimental model, we used Solanum tuberosum L. inocula. Within 8 weeks we found that, at the same light intensity, the vitroplantlets grown under white light LEDs are totally similar to those cultivated under CFL white light. We have also found that colored light, generated by LEDs, especially the red and blue ones, determine the tubergenesis at potato vitroplantlets in 6-8 weeks and at a normal sucrose concentration. This technique could be used to produce really fast and at low cost potato seedling and can be extended to any other plant, too.

  19. Development of a sparging technique for volatile emissions from potato (Solanum tuberosum)

    Science.gov (United States)

    Berdis, Elizabeth; Peterson, Barbara Vieux; Yorio, Neil C.; Batten, Jennifer; Wheeler, Raymond M.

    1993-01-01

    Accumulation of volatile emissions from plants grown in tightly closed growth chambers may have allelopathic or phytotoxic properties. Whole air analysis of a closed chamber includes both biotic and abiotic volatile emissions. A method for characterization and quantification of biogenic emissions solely from plantlets was developed to investigate this complex mixture of volatile organic compounds. Volatile organic compounds from potato (Solanum tuberosum L. cv. Norland) were isolated, separated and identified using an in-line configuration consisting of a purge and trap concentrator with sparging vessels coupled to a GC/MS system. Analyses identified plant volatile compounds: transcaryophyllene, alpha-humulene, thiobismethane, hexanal, cis-3-hexen-1-ol, and cis-3-hexenyl acetate.

  20. Clinostation influence on regeneration of cell wall in Solanum Tuberosum L. protoplasts

    Science.gov (United States)

    Nedukha, Elena M.; Sidorov, V. A.; Samoylov, V. M.

    1994-08-01

    Regeneration of cell walls in protoplasts was investigated using light- and electronmicroscopic methods. The protoplasts were isolated from mesophyll of Solanum tuberosum leaves and were cultivated on the horizontal low rotating clinostat (2 rpm) and in control for 10 days. Using a fluorescent method (with Calcofluor white) it was demonstrated that changes in vector gravity results in an regeneration inhibition of cell wall. With electron-microscopical and electro-cytochemical methods (staining with alcianum blue) dynamics of the regeneration of cell walls in protoplasts was studied; carbohydrate matrix of cell walls is deposited at the earliest stages of this process. The influence of microgravity on the cell wall regeneration is discussed in higher plants.

  1. Análisis de crecimiento en siete variedades de papa (solanum tuberosum l.

    Directory of Open Access Journals (Sweden)

    Fernando Borrego

    2000-01-01

    Full Text Available Análisis de crecimiento en siete variedades de papa (Solanum tuberosum L.. Se evaluaron siete variedades de papa (Solanum tuberosum L. Norteña, Gigant, Mondial, Snowden, Alpha, Atlantic y Russett Burbank, bajo criterios de eficiencia y productividad del análisis de crecimiento (seis muestreos, cada 18 días en promedio, como la tasa de crecimiento del cultivo (TCC, tasa de crecimiento relativo (TCR, tasa de crecimiento relativo foliar (TCRF, relación de área foliar (RAF, índice de area foliar (IAF y tasa de asimilación neta (TAN. Se encontraron diferencias (p≤0,01 para las variables en estudio (a excepción de la TAN, para las fuentes de variación de muestreos (seis a lo largo del ciclo siendo mayor la TCC en el cuarto muestreo con las variedades Norteña y Russett Burbank. La TCR fue mayor en Norteña, Russett Burbank y Alpha, habiendo un incremento considerable del quinto al sexto muestreo. En la RAF, los mejores genotipos fueron Atlantic y Snowden, mostrando Russett Burbank un pronunciado declive del quinto al sexto muestreo, por la senescencia del follaje. Por lo que respecta al IAF, los mejores genotipos fueron Norteña y Gigant, estableciéndose el máximo del cuarto al sexto muestreo, sin senescencia hasta ese muestreo. En la TAN, no se encontraron diferencias entre genotipos, mostrando superioridad la Norteña, siguiendo las otras cinco un patrón de comportamiento muy semejante

  2. Comportamiento del consumo de papa (Solanum tuberosum L. fresca en México Behavior of comsumption of fresh potato (Solanum tuberosum L. in Mexico

    Directory of Open Access Journals (Sweden)

    Adriana Sabbagh-Sánchez

    2011-08-01

    Full Text Available Los productores mexicanos de papa fresca (Solanum tuberosum L. sostienen actualmente, que un aumento en el consumo per capita sería la solución a los problemas relacionados con el mercado del tubérculo. El objetivo del presente estudio es conocer el comportamiento del consumo de papa fresca, en el largo plazo; por lo tanto, se formuló y estimó un modelo de ecuaciones simultáneas compuesto de dos ecuaciones de oferta, una de demanda, cuatro ecuaciones de precios y dos identidades. El modelo fue estimado por el método de mínimos cuadrados ordinarios, en dos etapas usando datos nacionales anuales en el periodo 1960-2006. Los resultados indicaron que de periodo 2000-2007 el consumo per capita anual de papa fresca fue de 16 kg por habitante, y que los factores que explican el comportamiento de esta variable fueron: el precio al consumidor de la papa (PCPR, el precio de la tortilla (PTR, el precio al consumidor del huevo (PCHR, el precio al consumidor del arroz (PCAR, el precio al consumidor de la carne de cerdo (PCCCR, el precio al consumidor de la carne de pollo (PCCPR, el presupuesto para el consumo real per capita (PCRP y la cantidad demandada de papa con un año de retraso (QDPt-1, con elasticidades de -0.2, 0.2, -0.1, -0.2, -0.004, -0.3, 0.2 y 0.13, respectivamente. La tendencia de los factores que afectan el consumo de papa fresca indicó que, en el largo plazo, el consumo per capita anual será de 20 kg por habitante, cifra muy inferior a más de 80 kg por habitante consumidos en la actualidad por los europeos; por lo tanto, los productores nacionales deben buscar dar valor agregado a la papa fresca, a través del procesamiento del producto.The mexican producers of fresh potatoes (Solanum tuberosum L., currently say that an increase in consumption per capita, would solve the problems related to the tuber-market. The aim of this paper is to understand the behavior of fresh potato consumption in long term; therefore, it was formulated

  3. Brilliant Green Dye Elimination from Water Using Psidium guajava Leaves and Solanum tuberosum Peels as Adsorbents in Environmentally Benign Way

    Directory of Open Access Journals (Sweden)

    Rabia Rehman

    2015-01-01

    Full Text Available The aim of this study is to check the feasibility of Psidium guajava (Guava leaves and peels of Solanum tuberosum (Potato as biosorbents in removal of Brilliant Green (BG in batch mode. Surface analysis of biosorbents was done by FT-IR and quantitatively analyzed by Boehm titration. The removal of dye was confirmed by UV-VIS spectroscopy. Isothermal modeling was studied by using Langmuir, Freundlich, and Temkin isotherms. Various isothermal parameters for adsorption of Brilliant Green such as qm=1.075 mg/g, 1.173 mg/g ΔG°=-3.397, and −2.397 KJ/mol were noted for Solanum tuberosum peels (PP and Psidium guajava leaves (GL, respectively. Similarly pH, moisture content, and various metals were quantitatively analyzed. Results showed that leaves of Psidium guajava were more effective for removal of Brilliant Green.

  4. Suberin of Potato (Solanum tuberosum Var. Nikola): Comparison of the Effect of Cutinase CcCut1 with Chemical Depolymerization

    OpenAIRE

    Jarvinen, Riikka; Silvestre, Armando J. D.; Holopainen, Ulla; Kaimainen, Mika; Nyyssola, Antti; Gil, Ana M.; Neto, Carlos Pascoal; Lehtinen, Pekka; Buchert, Johanna; Kallio, Heikki

    2009-01-01

    Chemical and enzymatic depolymerizations of suberin isolated from potato peel (Solanum tuberosum var. Nikola) were performed under various conditions. Enzymatic hydrolysis with cutinase CcCut1 and chemical methanolysis with NaOMe of suberin yielded monomeric fragments, which were identified as TMS derivatives with GC-MS and GC-FID. The solid, hydrolysis-resistant residues were analyzed with solid state (13)C CPMAS NMR, FT-IR, and microscopic methods. Methanolysis released more CHCl(13)-solubl...

  5. Brilliant Green Dye Elimination from Water Using Psidium guajava Leaves and Solanum tuberosum Peels as Adsorbents in Environmentally Benign Way

    OpenAIRE

    2015-01-01

    The aim of this study is to check the feasibility of Psidium guajava (Guava) leaves and peels of Solanum tuberosum (Potato) as biosorbents in removal of Brilliant Green (BG) in batch mode. Surface analysis of biosorbents was done by FT-IR and quantitatively analyzed by Boehm titration. The removal of dye was confirmed by UV-VIS spectroscopy. Isothermal modeling was studied by using Langmuir, Freundlich, and Temkin isotherms. Various isothermal parameters for adsorption of Brilliant Green such...

  6. Defense mechanisms of Solanum tuberosum L. in response to attack by plant-pathogenic bacteria

    Directory of Open Access Journals (Sweden)

    VERA A D POIATTI

    2009-01-01

    Full Text Available The natural resistance of plants to disease is based not only on preformed mechanisms, but also on induced mechanisms. The defense mechanisms present in resistant plants may also be found in susceptible ones. This study attempted to analyze the metabolic alterations in plants of the potato Solanum tuberosum L. cv. Agata that were inoculated with the incompatible plant-pathogenic bacteria X. axonopodis and R. solanacearum, and the compatible bacterium E. carotovora. Levels of total phenolic compounds, including the flavonoid group, and the activities of polyphenol oxidase (PPO and peroxidase (POX were evaluated. Bacteria compatibility was evaluated by means of infiltration of tubers. The defense response was evaluated in the leaves of the potato plants. Leaves were inoculated depending on their number and location on the stem. Multiple-leaf inoculation was carried out on basal, intermediate, and apical leaves, and single inoculations on intermediate leaves. Leaves inoculated with X. axonopodis and with R. solanacearum showed hypersensitive responses within 24 hours post-inoculation, whereas leaves inoculated with E. carotovora showed disease symptoms. Therefore, the R. solanacearum isolate used in the experiments did not exhibit virulence to this potato cultivar. Regardless of the bacterial treatments, the basal leaves showed higher PPO and POX activities and lower levels of total phenolic compounds and flavonoids, compared to the apical leaves. However, basal and intermediate leaves inoculated with R. solanacearum and X. axonopodis showed increases in total phenolic compounds and flavonoid levels. In general, multiple-leaf inoculation showed the highest levels of total phenolics and flavonoids, whereas the single inoculations resulted in the highest increase in PPO activity. The POX activity showed no significant difference between single- and multiple-leaf inoculations. Plants inoculated with E. carotovora showed no significant increase in

  7. Ultrastructural and physiological responses of potato (Solanum tuberosum L.) plantlets to gradient saline stress.

    Science.gov (United States)

    Gao, Hui-Juan; Yang, Hong-Yu; Bai, Jiang-Ping; Liang, Xin-Yue; Lou, Yan; Zhang, Jun-Lian; Wang, Di; Zhang, Jin-Lin; Niu, Shu-Qi; Chen, Ying-Long

    2014-01-01

    Salinity is one of the major abiotic stresses that impacts plant growth and reduces the productivity of field crops. Compared to field plants, test tube plantlets offer a direct and fast approach to investigate the mechanism of salt tolerance. Here we examined the ultrastructural and physiological responses of potato (Solanum tuberosum L. c.v. "Longshu No. 3") plantlets to gradient saline stress (0, 25, 50, 100, and 200 mM NaCl) with two consequent observations (2 and 6 weeks, respectively). The results showed that, with the increase of external NaCl concentration and the duration of treatments, (1) the number of chloroplasts and cell intercellular spaces markedly decreased, (2) cell walls were thickened and even ruptured, (3) mesophyll cells and chloroplasts were gradually damaged to a complete disorganization containing more starch, (4) leaf Na and Cl contents increased while leaf K content decreased, (5) leaf proline content and the activities of catalase (CAT) and superoxide dismutase (SOD) increased significantly, and (6) leaf malondialdehyde (MDA) content increased significantly and stomatal area and chlorophyll content decline were also detected. Severe salt stress (200 mM NaCl) inhibited plantlet growth. These results indicated that potato plantlets adapt to salt stress to some extent through accumulating osmoprotectants, such as proline, increasing the activities of antioxidant enzymes, such as CAT and SOD. The outcomes of this study provide ultrastructural and physiological insights into characterizing potential damages induced by salt stress for selecting salt-tolerant potato cultivars. PMID:25628634

  8. Light Spectral Quality Effects on the Growth of Potato (Solanum Tuberosum L.) Nodal Cuttings in Vitro

    Science.gov (United States)

    Wilson, Deborah A.; Weigel, Russell C.; Wheeler, Raymond M.; Sager, John C.

    1993-01-01

    The effects of light spectral quality on the growth of in vitro nodal cuttings of potato (Solanum tuberosum L.) cultivars Norland, Superior, Kennebec, and Denali were examined. The different light spectra were provided by Vita-Lite fluorescent (VF) (a white light control), blue fluorescent (BF), red fluorescent (RF), low-pressure sodium (LPS), and a combination of low-pressure sodium plus cool-white fluorescent lamps (LPS/CWF). For cultivars, stem lengths after 4 wks were longest under LPS, follow by RF, LPS/CWF, VF, and BF (in descending order). Microscopic studies revealed that cells were shortest when cultured in BF or VF environments, and were longest in RF or LPS lamp environments. The highest number axillary branches occurred on plantlets grown with LPS or LPS/CWF, whereas the lowest number occurred with BF. No leaf or stem edema (callus or gall-like growths) occurred iwth LPS or LPS/cwf lighting, and no edema occurred on cv. Norland plantlets, regardless of lighting. Results suggest that shoot morphologic development of in vitro grown potato plants can be controlled by controlling irradiant spectral quality.

  9. Photocatalytic activity of biogenic silver nanoparticles synthesized using potato (Solanum tuberosum) infusion

    Science.gov (United States)

    Roy, Kaushik; Sarkar, C. K.; Ghosh, C. K.

    2015-07-01

    In this study, we have reported a fast and eco-benign procedure to synthesis silver nanoparticle at room temperature using potato (Solanum tuberosum) infusion along with the study of its photocatalytic activity on methyl orange dye. After addition of potato infusion to silver nitrate solution, the color of the mixture changed indicating formation of silver nanoparticles. Time dependent UV-Vis spectra were obtained to study the rate of nanoparticle formation with time. Purity and crystallinity of the biogenic silver nanoparticles were examined by X-ray diffraction (XRD). Average size and morphology of the nanoparticles were characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Fourier transform infra-red spectroscopy (FTIR) was employed to detect functional bio-molecules responsible that contribute to the reduction and capping of biosynthesized Ag nanoparticles. Further, these synthesized nanoparticles were used to investigate their ability to degrade methyl orange dye under sunlight irradiation and the results showed effective photocatalytic property of these biogenic silver nanoparticles.

  10. Decline of rubisco activity and net photosynthesis in ozone-treated potato foliage. [Solanum tuberosum

    Energy Technology Data Exchange (ETDEWEB)

    Dann, M.S.; Pell, E.J.

    1987-04-01

    The effect of O/sub 3/ on rubisco activity in Solanum tuberosum L. cv Norland foliage was studied as related to oxidant-induced premature senescence. Plants, 25 days old, were exposed to O/sub 3/ increasing from 0.06 to 0.08 ..mu..1/L for 6 h/day for 4 days in a controlled environment chamber. On day 5 plants were exposed to a 6 h simulated inversion in which O/sub 3/ peaked at 0.12 /sup +/1/L. The authors measured initial and total rubisco activities and net photosynthesis of leaves at full expansion on days 0,3,5,6,9 and 12. These parameters declined in both ozone and control plants throughout the course of the experiment. O/sub 3/ exacerbated the decline and produced a significantly greater decrease following the inversion. The enhanced reduction in rubisco activity over time may be an important characteristics of ozone-induced premature senescence. Rubisco activation (initial/total activity) did not change with the treatment. The decrease in activity is most likely due to a decrease in available protein rather than a decrease in the percentage of rubisco activated in vivo.

  11. The subunit structure of potato tuber ADPglucose pyrophosphorylase. [Solanum tuberosum L

    Energy Technology Data Exchange (ETDEWEB)

    Okita, T.W.; Nakata, P.A.; Anderson, J.M. (Washington State Univ., Pullman (USA)); Sowokinos, J. (Univ. of Minnesota, St. Paul (USA)); Morell, M.; Preiss, J. (Michigan State Univ., East Lansing (USA))

    1990-06-01

    ADPglucose pyrophosphorylase has been extensively purified from potato (Solanum tuberosum L.) tuber tissue to study its structure. By employing a modified published procedure together with Mono Q chromatography, a near homogeneous enzyme preparation was obtained with substantial improvement in enzyme yield and specific activity. In single dimensional sodium dodecyl sulfate polyacrylamide gels, the enzyme migrated as a single polypeptide band with a mobility of about 50,000 daltons. Analysis by two-dimensional polyacrylamide gel electrophoresis, however, revealed the presence of two types of subunits which could be distinguished by their slight differences in net charge and molecular weight. The smaller potato tuber subunit was recognized by antiserum prepared against the smaller spinach leaf 51 kilodalton ADPglucose pyrophosphorylase subunit. In contrast, the anti-54 kilodalton raised against the spinach leaf subunit did not significantly react to the tuber enzyme subunits. The results are consistent with the hypothesis that the potato tuber ADPglucose pyrophosphorylase is not composed of a simple homotetramer as previously suggested, but is a product of two separate and distinct subunits as observed for the spinach leaf and maize enzymes.

  12. Superoxide dismutase, catalase, and. alpha. -tocopherol content of stored potato tubers. [Solanum tuberosum L

    Energy Technology Data Exchange (ETDEWEB)

    Spychalla, J.P.; Desborough, S.L. (Univ. of Minnesota, St. Paul (USA))

    1990-11-01

    Activated oxygen or oxygen free radical mediated damage to plants has been established or implicated in many plant stress situations. The extent of activated oxygen damage to potato (Solanum tuberosum L.) tubers during low temperature storage and long-term storage is not known. Quantitation of oxygen free radical mediated damage in plant tissues is difficult. However, it is comparatively easy to quantitate endogenous antioxidants, which detoxify potentially damaging forms of activated oxygen. Three tuber antioxidants, superoxide dismutase, catalase, and {alpha}-tocopherol were assayed from four potato cultivars stored at 3{degree}C and 9{degree}C for 40 weeks. Tubers stored at 3{degree}C demonstrated increased superoxide dismutase activities (up to 72%) compared to tubers stored at 9{degree}C. Time dependent increases in the levels of superoxide dismutase, catalase, and {alpha}-tocopherol occurred during the course of the 40 week storage. The possible relationship between these increases in antioxidants and the rate of activated oxygen production in the tubers is discussed.

  13. Metabolic Biosynthesis of Potato (Solanum tuberosum l.) Antioxidants and Implications for Human Health.

    Science.gov (United States)

    Lovat, Christie; Nassar, Atef M K; Kubow, Stan; Li, Xiu-Qing; Donnelly, Danielle J

    2016-10-25

    Potato (Solanum tuberosum L.) is common, affordable, readily stored, easily prepared for consumption, and nutritious. For these reasons, potato has become one of the top five crops consumed worldwide. Consequently, it is important to understand its contribution to both our daily and long-term health. Potato is one of the most important sources of antioxidants in the human diet. As such, it supports the antioxidant defense network in our bodies that reduces cellular and tissue toxicities that result from free radical-induced protein, lipid, carbohydrate, and DNA damage. In this way, potato antioxidants may reduce the risk for cancers, cardiovascular diseases, diabetes, and even radiation damage. A better understanding of these components of potato is needed by the food industry, health professionals, and consumers. This review provides referenced summaries of all of the antioxidant groups present in potato tubers and updated schematics including genetic regulation for the major antioxidant biosynthesis pathways. This review complements current knowledge on the role of potato in human health. We hope it will provide impetus toward breeding efforts to develop cultivars with increased antioxidant capacity as 'functional foods' and encourage potato consumers and processors to work toward preservation of antioxidant capacity in cooked potato and potato products. PMID:25674927

  14. Ultrastructural and physiological responses of potato (Solanum tuberosum L. plantlets to gradient saline stress

    Directory of Open Access Journals (Sweden)

    Hui-Juan eGao

    2015-01-01

    Full Text Available Salinity is one of the major abiotic stresses that impacts plant growth and reduces the productivity of field crops. Compared to field plants, test tube plantlets offer a direct and fast approach to investigate the mechanism of salt tolerance. Here we examined the ultrastructural and physiological responses of potato (Solanum tuberosum L. c.v. ‘Longshu No. 3’ plantlets to gradient saline stress (0, 25, 50, 100 and 200 mM NaCl with two consequent observations (two and six weeks, respectively. The results showed that, with the increase of external NaCl concentration and the duration of treatments, (1 the number of chloroplasts and cell intercellular spaces markedly decreased, (2 cell walls were thickened and even ruptured, (3 mesophyll cells and chloroplasts were gradually damaged to a complete disorganization containing more starch, (4 leaf Na and Cl contents increased while leaf K content decreased, (5 leaf proline content and the activities of catalase (CAT and superoxide dismutase (SOD increased significantly, and (6 leaf malondialdehyde (MDA content increased significantly and stomatal area and chlorophyll content decline were also detected. Severe salt stress (200 mM NaCl inhibited plantlet growth. These results indicated that potato plantlets adapt to salt stress to some extent through accumulating osmoprotectants, such as proline, increasing the activities of antioxidant enzymes, such as CAT and SOD. The outcomes of this study provide ultrastructural and physiological insights into characterizing potential damages induced by salt stress for selecting salt-tolerant potato cultivars.

  15. Polyphenol oxidase expression in potato (Solanum tuberosum) tubers inhibited to sprouting by treatment with iodine atmosphere.

    Science.gov (United States)

    Eolini, Francesco; Hochkoeppler, Alejandro; Credi, Andrea; Rodríguez, Antonio Gonzàlez Vara Y; Poggi, Valeria

    2004-08-01

    Iodine-saturated atmosphere was found to inhibit the sprouting of potato (Solanum tuberosum L.) tubers. The iodine concentration in tuber tissues increased as a function of exposure length, and the onset of inhibition of sprouting was found to depend on tubers genotype. During the time-course of the treatment, the transcription of polyphenol oxidases (EC 1.10.3.1 and EC 1.14.18.1) was undetectable in tuber peel, whereas in bud tissues featured an increase, followed by a decrease occurring simultaneously with the suppression of sprouting. The treatment of tubers with iodine strongly affected the expression of polyphenol oxidases at the transcriptional level. Polyphenol oxidase activity in buds poorly reflected the corresponding level of transcription; similarly, little differences were found among the enzyme isoforms expressed in buds as a function of length of exposure to iodine. These findings suggest that the induction of polyphenol oxidases mRNAs transcription could probe the inhibition of sprouting by iodine. PMID:15587701

  16. An Improved Method of Optimizing the Extraction of Polyphenol Oxidase from Potato (Solanum tuberosum L. Peel

    Directory of Open Access Journals (Sweden)

    Suprabhat MUKHERJEE

    2012-02-01

    Full Text Available The present study has an objective to optimize the extraction of Polyphenol Oxidase (PPO from potato (Solanum tuberosum L. peel. Response surface methodology (RSM was used to design experiments and study the effect of six influential extraction parameters: extraction buffer concentration (100-500 mM, pH of extraction buffer (4.5-8.5, time (1-12 hours, temperature (4-40�C, concentration of PMSF (1-5 mM and volume of extraction buffer (200-1000 ml on the extraction of PPO. The dependent variable was considered as response function which was specific activity (SA of extracted PPO. ANOVA was performed to obtain the regression equation that could predict the responses within given range. From RSM generated model, the optimum conditions for the maximum extraction of PPO were phosphate buffer concentration of 100 mm, buffer pH of 4.5, extraction time of 1 hour, 40�C temperature, PMSF concentration of 5 mM and buffer volume of 200 ml. Finally, this study illustrates a cost effective and less time consuming method to maximize the extraction of PPO from a vegetable waste.

  17. Physico-chemical and sensory evaluation of potato (Solanum tuberosum L.) after irradiation.

    Science.gov (United States)

    Soares, Ivanesa G M; Silva, Edvane B; Amaral, Ademir J; Machado, Erilane C L; Silva, Josenilda M

    2016-06-01

    This work evaluated the effects of ionizing radiation on the physico-chemical and sensory characteristics of the potato cultivar Ágata (Solanum tuberosum L.), including budding and deterioration, with the end goal of increasing shelf life. For this, four groups of samples were harvested at the maturation stage. Three of them were separately exposed to a Co-60 source, receiving respective doses of 0.10, 0.15 and 2.00 kGy, while the non-irradiated group was kept as a control. All samples were stored for 35 days at 24 °C (± 2) and at 39% relative humidity. The following aspects were evaluated: budding, rot, loss of weight, texture, flesh color, moisture, external and internal appearance, aroma, soluble solids, titratable acidity, vitamin C, protein, starch and glucose. The results indicated that 0.15 kGy was the most effective dose to reduce sprouting and post-harvest losses, under the conditions studied. PMID:27276382

  18. Structure of the polyphenolic component of suberin isolated from potato (Solanum tuberosum var. Nikola).

    Science.gov (United States)

    Mattinen, Maija-Liisa; Filpponen, Ilari; Järvinen, Riikka; Li, Bin; Kallio, Heikki; Lehtinen, Pekka; Argyropoulos, Dimitris

    2009-10-28

    Suberin is present in the underground parts of vegetables and in the bark of trees. Characterization of suberin and the structure of its polyphenolic component have been hampered by insolubility of the polymers. Thus, enzymatically isolated and extractive free suberin enriched fraction from potato, Solanum tuberosum var. Nikola, and the chemically further fractionated phenolics were characterized in solid state by FTIR, DSC, and elemental analysis to identify the groups and to verify success of isolation. For MW and quantitative determination of the groups, polymers were solubilized in ionic liquid derivatized and analyzed by GPC and (31)P NMR. Suberin enriched fraction, MW = ca. 44 x 10(3) g/mol, is a mixture of carbohydrates and polyesters of aliphatic long chain hydroxy fatty acids and diacids linked via ester bonds to the phenolics, MW = ca. 27 x 10(3) g/mol, formed by guaiacyl- and p-hydroxyphenyl structures. Phenolics in peels may be important sources of antioxidants for various applications. PMID:19785417

  19. Patatin, the tuber storage protein of potato (Solanum tuberosum L.), exhibits antioxidant activity in vitro.

    Science.gov (United States)

    Liu, Yen-Wenn; Han, Chuan-Hsiao; Lee, Mei-Hsien; Hsu, Feng-Lin; Hou, Wen-Chi

    2003-07-16

    The potato (Solanum tuberosum L.) tuber storage protein, patatin, was purified to homogeneity with a molecular mass of 45 kDa. The purified patatin showed antioxidant or antiradical activity by a series of in vitro tests, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical (half-inhibition concentration, IC(50), was 0.582 mg/mL) scavenging activity assays, anti-human low-density lipoprotein peroxidation tests, and protections against hydroxyl radical-mediated DNA damages and peroxynitrite-mediated dihydrorhodamine 123 oxidations. Using electron paramagnetic resonance (EPR) spectrometry for hydroxyl radical detections, it was found that the intensities of the EPR signal were decreased by the increased amounts of patatin added (IC(50) was 0.775 mg/mL). Through modifications of patatin by iodoacetamide or N-bromosuccinimide, it was found that the antiradical activities of modified patatin against DPPH or hydroxyl radicals were decreased. It was suggested that cysteine and tryptophan residues in patatin might contribute to its antioxidant activities against radicals. PMID:12848515

  20. Genome-wide analysis and expression profiling of the Solanum tuberosum aquaporins.

    Science.gov (United States)

    Venkatesh, Jelli; Yu, Jae-Woong; Park, Se Won

    2013-12-01

    Aquaporins belongs to the major intrinsic proteins involved in the transcellular membrane transport of water and other small solutes. A comprehensive genome-wide search for the homologues of Solanum tuberosum major intrinsic protein (MIP) revealed 41 full-length potato aquaporin genes. All potato aquaporins are grouped into five subfamilies; plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), NOD26-like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs) and x-intrinsic proteins (XIPs). Functional predictions based on the aromatic/arginine (ar/R) selectivity filters and Froger's positions showed a remarkable difference in substrate transport specificity among subfamilies. The expression pattern of potato aquaporins, examined by qPCR analysis, showed distinct expression profiles in various organs and tuber developmental stages. Furthermore, qPCR analysis of potato plantlets, subjected to various abiotic stresses revealed the marked effect of stresses on expression levels of aquaporins. Taken together, the expression profiles of aquaporins imply that aquaporins play important roles in plant growth and development, in addition to maintaining water homeostasis in response to environmental stresses. PMID:24215931

  1. Product stability and sequestration mechanisms in Solanum tuberosum engineered to biosynthesize high value ketocarotenoids.

    Science.gov (United States)

    Mortimer, Cara L; Misawa, Norihiko; Ducreux, Laurence; Campbell, Raymond; Bramley, Peter M; Taylor, Mark; Fraser, Paul D

    2016-01-01

    To produce commercially valuable ketocarotenoids in Solanum tuberosum, the 4, 4' β-oxygenase (crtW) and 3, 3' β-hydroxylase (crtZ) genes from Brevundimonas spp. have been expressed in the plant host under constitutive transcriptional control. The CRTW and CRTZ enzymes are capable of modifying endogenous plant carotenoids to form a range of hydroxylated and ketolated derivatives. The host (cv. Désirée) produced significant levels of nonendogenous carotenoid products in all tissues, but at the apparent expense of the economically critical metabolite, starch. Carotenoid levels increased in both wild-type and transgenic tubers following cold storage; however, stability during heat processing varied between compounds. Subcellular fractionation of leaf tissues revealed the presence of ketocarotenoids in thylakoid membranes, but not predominantly in the photosynthetic complexes. A dramatic increase in the carotenoid content of plastoglobuli was determined. These findings were corroborated by microscopic analysis of chloroplasts. In tuber tissues, esterified carotenoids, representing 13% of the total pigment found in wild-type extracts, were sequestered in plastoglobuli. In the transgenic tubers, this proportion increased to 45%, with esterified nonendogenous carotenoids in place of endogenous compounds. Conversely, nonesterified carotenoids in both wild-type and transgenic tuber tissues were associated with amyloplast membranes and starch granules. PMID:25845905

  2. ACUMULACIÓN Y DISTRIBUCIÓN DE MATERIA SECA DE CUATRO VARIEDADES DE PAPA (Solanum tuberosum L.) EN ZIPAQUIRÁ, CUNDINAMARCA (COLOMBIA) DRY MATTER ALLOCATION AND PARTITIONING OF FOUR POTATO VARIETIES (Solanum tuberosum L.) IN ZIPAQUIRÁ, CUNDINAMARCA (COLOMBIA)

    OpenAIRE

    Carlos Eduardo Ñústez López; Marcela Santos Castellanos; Mariela Segura Abril

    2009-01-01

    Esta investigación evaluó la acumulación y distribución de materia seca en los diferentes órganos de la planta de cuatro variedades de papa (Solanum tuberosum L.) en el municipio de Zipaquirá (Cundinamarca) uno de los mayores productores de papa en Colombia. Se evaluaron las variedades Betina, Pastusa Suprema y Esmeralda (desarrolladas por el programa de mejoramiento genético de papa de la Facultad de Agronomía de la Universidad Nacional de Colombia) y la variedad Diacol Capiro, que es una de...

  3. Application time of nitrogen fertilizer 15N by a potato crop (Solanum Tuberosum L.)

    International Nuclear Information System (INIS)

    This study was performed at the ''San Jorge'' experimental farm of the Instituto Colombiano Agropecuario (ICA), Bogota, Colombia. The study was performed to investigate the effect of timing of application of nitrogen fertilizer on the productivity of, and the efficiency of utilization of 15N-labelled fertilizer by, a potato crop (Solanum tuberosum L.), cv. Tequendama. The crop was fertilized with 100, 200 and 100 Kg/ha-1 of N, P2O5 and K2O respectively. The N fertilizers were either added as 15N labelled urea (2.955 at.% 15N excess) or as labelled ammonium sulphate (2.071 at.% 15N excess). In all treatments with nitrogen, a total of 100 Kg N ha-1 was added, but the nitrogen was added either in two or three split doses (only one dose being labelled with 15N) at the following times: at planting, 35 days after emergence (DAE) and/or 60 DAE. It was found that: a) Nitrogen fertilization increased tuber production from 24 to 43 t/ha-1; b) The tubers constituted approximately 80% of total plant dry matter and 70% of the total nitrogen and fertilizer N accumulated by the plant; c) The fertilizer use efficiency varied between 49 and 68%, and the highest efficiency occurred when the nitrogen was split in three doses; d) The urea and ammonium sulphate gave similar results in all parameters evaluated; e) When the total nitrogen difference method was applied to interpretation of the results the fertilizer use efficiency was overestimated by 15 to 30%

  4. Population Dynamics of Soil Pseudomonads in the Rhizosphere of Potato (Solanum tuberosum L.).

    Science.gov (United States)

    Loper, J E; Haack, C; Schroth, M N

    1985-02-01

    Rhizosphere population dynamics of seven Pseudomonas fluorescens and Pseudomonas putida strains isolated from rhizospheres of various agricultural plants were studied on potato (Solanum tuberosum L.) in field soil under controlled environmental conditions. Rhizosphere populations of two strains (B10 and B4) were quantitatively related to initial seed piece inoculum levels when plants were grown at -0.3 bar matric potential. At a given inoculum level, rhizosphere populations of strain B4 were consistently greater than those of strain B10. In vivo growth curves on 4-cm root tip-proximal segments indicated that both strains grew at similar rates in the potato rhizosphere, but large populations of strain B10 were not maintained at 24 degrees C after 7 h, whereas those of strain B4 were maintained for at least 40 h. Although both strains grew more rapidly in the rhizosphere at 24 degrees C than at 12 degrees C, their rhizosphere populations after seed piece inoculation were generally greater at 12 or 18 degrees C, indicating that in vivo growth did not solely determine rhizosphere populations in these studies. In vitro osmotolerance of seven Pseudomonas strains (including strains B4 and B10) was correlated with their abilities to establish stable populations in the rhizosphere of potato. Stability of rhizosphere populations of the Pseudomonas strains studied here was maximized at low (i.e., 12 degrees C) soil temperatures. These results indicate that Pseudomonas strains differ in their capacity to maintain stable rhizosphere populations in association with potato. This capacity, distinct from the ability to grow in the rhizosphere, may limit the establishment of rhizosphere populations under some environmental conditions. PMID:16346729

  5. Chlorogenic Acid Biosynthesis Appears Linked with Suberin Production in Potato Tuber (Solanum tuberosum).

    Science.gov (United States)

    Valiñas, Matías Ariel; Lanteri, María Luciana; ten Have, Arjen; Andreu, Adriana Balbina

    2015-05-20

    Potato (Solanum tuberosum L.) is a good source of dietary antioxidants. Chlorogenic acid (CGA) and caffeic acid (CA) are the most abundant phenolic acid antioxidants in potato and are formed by the phenylpropanoid pathway. A number of CGA biosynthetic routes that involve hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) and/or hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT) have been proposed, but little is known about their path in potato. CA production requires a caffeoyl shikimate esterase (CSE), and CA serves as a substrate of lignin precursor ferulic acid via the action of caffeic/5-hydroxyferulic acid O-methyltransferase (COMT I). CGA is precursor of caffeoyl-CoA and, via caffeoyl-CoA O-methyltransferase (CCoAOMT), of feruloyl-CoA. Feruloyl-CoA is required for lignin and suberin biosynthesis, crucial for tuber development. Here, metabolite and transcript levels of the mentioned and related enzymes, such as cinnamate 4-hydroxylase (C4H), were determined in the flesh and skin of fresh and stored tubers. Metabolite and transcript levels were higher in skin than in flesh, irrespective of storage. CGA and CA production appear to occur via p-coumaroyl-CoA, using HQT and CSE, respectively. HCT is likely involved in CGA remobilization toward suberin. The strong correlation between CGA and CA, the correspondence with C4H, HQT, CCoAOMT2, and CSE, and the negative correlation of HCT and COMT I in potato tubers suggest a major flux toward suberin. PMID:25921651

  6. Growth and tuberization of potato (Solanum tuberosum L.) under continuous light

    Science.gov (United States)

    Wheeler, R. M.; Tibbitts, T. W.

    1986-01-01

    The growth and tuberization of potatoes (Solanum tuberosum L.) maintained for 6 weeks under four different regimes of continuous irradiance were compared to plants given 12 hours light and 12 hours dark. Treatments included: (a) continuous photosynthetic photon flux of 200 micromoles per square meter per second cool-white fluorescent (CWF); (b) continuous 400 micromoles per square meter per second CWF; (c) 12 hours 400 micromoles per square meter per second CWF plus 12 hours dim CWF at 5 micromoles per square meter per second; (d) 12 hours [400] micromoles per square meter per second CWF plus 12 hours dim incandescent (INC) at 5 micromoles per square meter per second and a control treatment of 12 hours light at 400 micromoles per square meter per second CWF and 12 hours dark. The study included five cultivars ranging from early- to late-season types: 'Norland,' 'Superior,''Norchip,' 'Russet Burbank,' and 'Kennebec,' Tuber development progressed well under continuous irradiation at 400 micromoles per square meter per second and under 12 hours irradiance and 12 hours dark, while tuber development was suppressed in all other light treatments. Continuous irradiation at 200 or 400 micromoles per square meter per second resulted in severe stunting and leaf malformation on 'Superior' and 'Kennebec' plants, but little or no injury and vigorous shoot growth in the other cultivars. No injury or stunting were apparent under 12-dim light or 12-dark treatments. Plants given 12 hours dim INC showed significantly greater stem elongation but less total biomass than plants in other treatments. The continuous light encouraged shoot growth over tuber growth but this trend was overridden by providing a high irradiance level. The variation among cultivars for tolerance to continuous lighting indicates that potato may be a useful species for photoinhibition studies.

  7. Thermal control of some post-harvest rot pathogens of Irish potato (solanum tuberosum l.

    Directory of Open Access Journals (Sweden)

    Salami Olusola Abiodun

    2007-01-01

    Full Text Available Thermal control effect on the incidence of some post-harvest rot pathogens of Solanum tuberosum (potato was investigated in this study. Three cultivars of potato tuber whose local names are, Patiska, Mai Bawondoya and Nicola were used for the study. Five pathogenic fungi viz: Botryodiplodia theobromae, Fusarium redolens, Fusarium oxysporum, Penicillium sp. and Rhizopus oryzae associated with post harvest storage rot of root-tubers, were isolated from diseased potatoes. Among the three species of potatoes used in the study, Patiska was found most resistant followed by Mai Bawondoya, while Nicola was the least resistant. Increase in substrate (i.e. soluble starch or CMC concentration enhanced a proportional increase in mycelial growth and in the amount of extracellular enzymes produced. Some of these test pathogens were found to produce cell wall degrading enzymes (i.e. amylase and cellulase. Preferential utilization of carbohydrate sources was established in this study based on the growth of test pathogens. Growth on potato broth medium was highest followed by growth on Cocoyam and Sweet potato broth media and least on Cassava broth medium. Growth of the test pathogens on carbohydrate sources was found at variant. The use of hot water treatment at different temperatures was found to significantly reduce post-harvest fungal populations on the surface of root-tubers. The efficacy of blanching in hot water at 60˚C was significantly higher than that of blanching in hot water at other temperatures. The control method adopted in this study showed that the problems of potatoes’ rot disease in storage (especially by the peasant farmers can be eradicated by thermal treatments without reducing the quality of the Irish tuber.

  8. Evaluation of Soil and Plant Nitrogen Tests in Potato (Solanum tuberosum L. Production

    Directory of Open Access Journals (Sweden)

    Boris Lazarević

    2014-05-01

    Full Text Available Nitrogen (N management is critical in optimizing potato (Solanum tuberosum L. yield and reducing environmental pollution. Several plant and soil based tests were proposed for assessment of N status in crop plants. Aim of this study was to evaluate the convenience of plant based tests (leaf chlorophyll content index (CCI and petiole nitrate-nitrogen (NO3-N concentrations and soil based test (soil nitrogen (Nmin content for estimating potato N status. Experiment was conducted in North West region of Croatia in 2011 growing season as randomized complete block design with four replications. Treatments were four N rates, 50, 100, 150 and 200 kg N ha-1. At pre-plant 50 kg N ha-1 was applied as NPK 7:20:30. Four side-dressed N rates, 0, 50, 100 and 150 kg N ha-1 were applied 45 days after planting (DAP. CCI and petiole NO3-N concentrations were measured at 58 and 98 (DAP, while soil Nmin content was measured before planting, 58 DAP, 98 DAP and after harvest. No significant differences in total tuber yield were determined between 100, 150 and 200 kg N ha-1 treatments. Significantly highest residual Nmin content (33.25 kg ha-1 after harvest was determined on plots fertilized with 200 kg N ha-1, indicating potential N leaching during fall and winter. Significant linear relationship between petiole N-NO3 and N treatments were determined at 58 DAP (R2=0.32 and 98 DAP (R2=0.75 and between CCI and N treatments at 98 DAP (R2=0.62. Significant linear relationship between soil Nmin content and N treatments were determined at 58 (R2=0.57 and 98 DAP (R2=0.56. Plant based tests are better correlated with N treatments at later growth stages in which applied N could be utilized by the crop.

  9. Catalase inhibition accelerates dormancy release and sprouting in potato (Solanum tuberosum L. tubers

    Directory of Open Access Journals (Sweden)

    Mohammed Bajji

    2007-01-01

    Full Text Available The involvement of hydrogen peroxide (H2O2 metabolism in dormancy release and sprouting of potato (Solanum tuberosum L. tubers has been investigated using three complementary approaches. In the first approach, the evolution of the sprouting kinetics, H2O2 content and antioxidant enzyme activities were examined during tuber storage. The most important changes occurred at the « bud/sprout » level. In particular, dormancy release was accompanied by a transient but remarkable increase in H2O2 content. In the second approach, the effect of a catalase (CAT, EC 1.11.1.6 inhibitor (thiourea or of exogenous H2O2 application on tuber sprouting behaviour was assessed. Both treatments resulted in a reduction of the dormancy period and in rapid and synchronised sprouting of the treated tubers when compared to the control as well as in increased sprout number per tuber. In the third approach, the effect of CAT inhibition on potato tuber dormancy and sprouting was evaluated using the transgenic technology. Plants partially repressed in their CAT activity were produced and, once again, CAT inhibition resulted in acceleration of the sprouting kinetics and in increased sprout number of the transgenic tubers compared to those from the wild type. It thus appears that tuber dormancy and sprouting can be controlled in potato by the manipulation of H2O2 metabolism via the inhibition of CAT activity. The possible mechanisms whereby CAT inhibitors or H2O2 overcome dormancy and promote sprouting in the potato tuber are discussed in relation to what is known in other plant models (seeds and fruit tree buds.

  10. Characterization of a Kunitz-type serine protease inhibitor from Solanum tuberosum having lectin activity.

    Science.gov (United States)

    Shah, Kunal R; Patel, Dhaval K; Pappachan, Anju; Prabha, C Ratna; Singh, Desh Deepak

    2016-02-01

    Plant lectins and protease inhibitors constitute a class of proteins which plays a crucial role in plant defense. In our continuing investigations on lectins from plants, we have isolated, purified and characterized a protein of about 20 kDa, named PotHg, showing hemagglutination activity from tubers of Indian potato, Solanum tuberosum. De novo sequencing and MS/MS analysis confirmed that the purified protein was a Kunitz-type serine protease inhibitor having two chains (15 kDa and 5 kDa). SDS and native PAGE analysis showed that the protein was glycosylated and was a heterodimer of about 15 and 5 kDa subunits. PotHg agglutinated rabbit erythrocytes with specific activity of 640 H.U./mg which was inhibited by complex sugars like fetuin. PotHg retained hemagglutination activity over a pH range 4-9 and up to 80°C. Mannose and galactose interacted with the PotHg with a dissociation constant (Kd) of 1.5×10(-3) M and 2.8×10(-3) M, respectively as determined through fluorescence studies. Fluorescence studies suggested the involvement of a tryptophan in sugar binding which was further confirmed through modification of tryptophan residues using N-bromosuccinimide. Circular dichroism (CD) studies showed that PotHg contains mostly β sheets (∼45%) and loops which is in line with previously characterized protease inhibitors and modeling studies. There are previous reports of Kunitz-type protease inhibitors showing lectin like activity from Peltophorum dubium and Labramia bojeri. This is the first report of a Kunitz-type protease inhibitor showing lectin like activity from a major crop plant and this makes PotHg an interesting candidate for further investigation. PMID:26645142

  11. Acclimation of photosynthesis to elevated CO sub 2 in five C sub 3 species. [Chenopodium album, Phaseolus vulgaris, Solanum tuberosum, Solanum melongena, Brassica oleracea

    Energy Technology Data Exchange (ETDEWEB)

    Sage, R.F. (Univ. of Georgia, Athens (USA)); Sharkey, T.D. (Univ. of Wisconsin, Madison (USA)); Seemann, J.R. (Univ. of Nevada, Reno (USA))

    1989-02-01

    The effect of long-term (weeks to months) CO{sub 2} enhancement on (a) the gas-exchange characteristics, (b) the content and activation state of ribulose-1,5-bisphosphate carboxylase (rubisco), and (c) leaf nitrogen, chlorophyll, and dry weight per area were studied in five C{sub 3} species (Chenopodium album, Phaseolus vulgaris, Solanum tuberosum, Solanum melongena, and Brassica oleracea) grown at CO{sub 2} partial pressures of 300 or 900 to 1000 microbars. Long-term exposure to elevated CO{sub 2} affected the CO{sub 2} response of photosynthesis in one of three ways: (a) the initial slope of the CO{sub 2} response was unaffected, but the photosynthetic rate at high CO{sub 2} increased (S. tuberosum); (b) the initial slope decreased but the CO{sub 2}-saturated rate of photosynthesis decreased (B. oleracea, S. melongena). In all five species, growth at high CO{sub 2} increased the extent to which photosynthesis was stimulated following a decrease in the partial pressure of O{sub 2} or an increase in measurement CO{sub 2} above 600 microbars. This stimulation indicates that a limitation on photosynthesis by the capacity to regenerate orthophosphate was reduced or absent after acclimation to high CO{sub 2}. Leaf nitrogen per area either increased (S. tuberosum, S. melongena) or was little changed by CO{sub 2} enhancement. The content of rubisco was lower in only two of the fives species, yet its activation state was 19% to 48% lower in all five species following long-term exposure to high CO{sub 2}. These results indicate that during growth in CO{sub 2}-enriched air, leaf rubisco content remains in excess of that required to support the observed photosynthetic rates.

  12. Comparative effects of partial root-zone drying and deficit irrigation on nitrogen uptake in potatoes (Solanum tuberosum L.)

    DEFF Research Database (Denmark)

    Wang, Huiqun; Liu, Fulai; Andersen, Mathias Neumann;

    2009-01-01

    The effects of partial root-zone drying (PRD) as compared with deficit irrigation (DI) and full irrigation (FI) on nitrogen (N) uptake and partitioning in potato (Solanum tuberosum L.) were investigated. Potato plants were grown in split-root pots and were exposed to FI, PRD, and DI treatments at...

  13. Isolation of a gene encoding a copper chaperone for the Cu/Zn superoxide dismutase and characterization of its promoter in Solanum tuberosum L.

    NARCIS (Netherlands)

    Trindade, L.M.; Horvath, B.M.; Bergervoet-van Deelen, J.E.M.; Visser, R.G.F.

    2003-01-01

    Gene expression during the potato (Solanum tuberosum) tuber lifecycle was monitored by cDNA-amplified fragment-length polymorphism, and several differentially expressed transcript-derived fragments were isolated. One fragment, named TDFL431, showed high homology to a copper (Cu) chaperone for Cu/zin

  14. First report of ‘Candidatus Phytoplasma asteris’ subgroup 16SrI-A associated with a disease of potato (Solanum tuberosum) in Lithuania

    Science.gov (United States)

    Symptoms of little leaf, witches’-broom, and abnormally small and deformed potatoes, suggestive of possible phytoplasmal infection, were observed in diseased potato (Solanum tuberosum var. Hannibal arba Kestrel) in the Vilnius region of Lithuania. DNA extracted from symptomatic leaves and shoots we...

  15. EFECTO DEL QUITOMAX EN EL CRECIMIENTO Y RENDIMIENTO DEL CULTIVO DE LA PAPA (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Donaldo Morales Guevara

    2015-01-01

    Full Text Available El objetivo del trabajo fue evaluar algunos componentes del crecimiento y el rendimiento en plantas de papa (Solanum tuberosum L. sometidas a la aplicación foliar de diferentes dosis de QuitoMax (bioproducto a base de polímeros de quitosano en dos momentos del desarrollo del cultivo. El trabajo se realizó durante tres ciclos del cultivo (2009-2010; 2010-2011 y 2011-2012. En el primer ciclo se contó con cuatro tratamientos en los que, además del control en el que no se aplicó el producto, se utilizaron dos en los que se empleó una dosis de 300 mg ha-1 a los 30 o a los 50 días posteriores a la plantación y otro en el que se aplicaron dos dosis de 150 mg ha-1 una a los 30 días y la otra a los 50 días posteriores a la plantación. En los dos ciclos siguientes se contó además del control, con nueve tratamientos en los que se aplicaron dosis de 100, 300 y 500 mg ha-1, tanto a los 30 como a los 50 días posteriores a la plantación y otros tres en los que se aplicaron 50, 150 y 250 mg ha-1 en los dos momentos antes señalados. Las variables evaluadas fueron la longitud y diámetro de los tallos, el número de hojas por planta, el número de tubérculos por planta, la masa fresca promedio de los tubérculos y su porcentaje de materia seca. Se estimaron los rendimientos en base a las masas fresca y seca de los tubérculos. El análisis de los resultados mostró una mejor respuesta de las plantas cuando recibieron dos aplicaciones de QuitoMax, destacándose el tratamiento en el que las plantas recibieron 150 mg ha-1,el que provocó un aumento del rendimiento superior a un 15 % en relación al control no aplicado.

  16. Subsurface irrigation of potato crop (Solanum tuberosum ssp. Andigena) in Suka Kollus with different drainage systems

    Science.gov (United States)

    Serrano-Coronel, Genaro; Chipana-Rivera, René; Fátima Moreno-Pérez, María; Roldán-Cañas, José

    2016-04-01

    Among the most important hydraulic structures of pre-Hispanic ancestral technology developed in the Andean region, we find the suka kollus, aymara word, called also waru waru, en quechua or raised fields, in English. They are raised platforms surrounded by water canals that irrigate subsurface, but also have the function of draining, to deal with floods because they are surrounding Lake Titicaca. They also have the property of generating a thermoregulatory effect to crops, depending on the configuration of the channels and platforms. Such agro-ecosystems are being abandoned, however, if properly addressed crop management and some drainage canals are replaced by underground drains for increased crop area could be very useful in enabling marginal soils affected by salts and / or excess water. For these reasons, the objective of this study was to evaluate the subsurface irrigation in the potato crop in suka kollus under a system of surface drainage, and mixed drainage (surface and subsurface). The study was conducted in marginal soils of Kallutaca area, located 30 km from the city of La Paz, Bolivia, at a height of 3892 m.a.s.l. The cultivation of the potato (Solanum tuberosum ssp. Andigena) was used. Four treatments were tested with different widths of the platforms: T1 (Control) with drainage through channels; T2 (replacing a channel by a drain); T3 (replacing two channels by two drains); T4 (replacing three channels by three drains). The flow of water into the soil from the water table was predominantly upward, except during periods of high rainfall. In terms of treatments, the flow in T1 was higher, mainly at weeks 8 to 11 after seedling emergence, coinciding with the phenological phases of flowering and at the beginning of the tuber ripening. It was followed by T3, T2 and T4 treatments, respectively. Tuber yield, if one considers that the channels detract arable land, was higher in the T3 treatment,16.4 Mg / ha, followed by T2 treatment, 15.2 Mg / ha, T1

  17. Hyperspectral remote sensing for advanced detection of early blight (Alternaria solani) disease in potato (Solanum tuberosum) plants

    Science.gov (United States)

    Atherton, Daniel

    Early detection of disease and insect infestation within crops and precise application of pesticides can help reduce potential production losses, reduce environmental risk, and reduce the cost of farming. The goal of this study was the advanced detection of early blight (Alternaria solani) in potato (Solanum tuberosum) plants using hyperspectral remote sensing data captured with a handheld spectroradiometer. Hyperspectral reflectance spectra were captured 10 times over five weeks from plants grown to the vegetative and tuber bulking growth stages. The spectra were analyzed using principal component analysis (PCA), spectral change (ratio) analysis, partial least squares (PLS), cluster analysis, and vegetative indices. PCA successfully distinguished more heavily diseased plants from healthy and minimally diseased plants using two principal components. Spectral change (ratio) analysis provided wavelengths (490-510, 640, 665-670, 690, 740-750, and 935 nm) most sensitive to early blight infection followed by ANOVA results indicating a highly significant difference (p potato plants.

  18. Alternative oxidase of potato is an integral membrane protein synthesized de novo during aging of tuber slices. [Solanum tuberosum

    Energy Technology Data Exchange (ETDEWEB)

    Hiser, C.; McIntosh, L. (Michigan State Univ., East Lansing (USA))

    1990-05-01

    The rise in alternative respiratory capacity upon aging of potato (Solanum tuberosum) tuber slices is correlated with changes in mitochondrial membrane protein composition and a requirement for cytoplasmic protein synthesis. However, the lack of an antibody specific to the alternative oxidase has, until recently, prevented examination of the alternative oxidase protein(s) itself. We have employed a monoclonal antibody raised against the Sauromatum guttatum alternative oxidase to investigate developmental changes in the alternative pathway of aging potato slice mitochondria and to characterize the potato alternative oxidase by one- and two-dimensional gel electrophoresis. The relative levels of a 36 kilodalton protein parallel the rise in alternative path capacity. A plausible interpretation is that this alternative oxidase protein is synthesized de novo during aging of potato slices.

  19. Foliar application of chlorocholine chloride improves leaf mineral nutrition, antioxidant enzyme activity, and tuber yield of potato (Solanum tuberosum L.)

    DEFF Research Database (Denmark)

    Wang, Huigun; Xiao, Langtao; Tong, Jianhua;

    2010-01-01

    In southern China, potatoes (Solanum tuberosum L.) are grown in the late season and the crops are often subjected to low temperature stress particularly during the tuber bulking stage. Exogenous chlorocholine chloride (CCC) treatment has been found to improve crop performance under suboptimal...... growth conditions; however, the physiological mechanisms underlying the beneficial effects have not been fully understood. The objective of this study was to investigate the effects of CCC treatment on mineral nutrition, antioxidant enzyme system, and tuber yield of potato (cv. Zhongshu 3) under field...... conditions. The plants were foliar sprayed twice with 1.5, 2.0 and 2.5 g l-1 CCC at 24 and 28 days after emergence (DAE), respectively; and plants without CCC treatment were serviced as control. Leaf samples were collected on 56 DAE for determination of mineral nutrition contents and antioxidant enzyme...

  20. Expression of an isoflavone reductase-like gene enhanced by pollen tube growth in pistils of Solanum tuberosum.

    Science.gov (United States)

    van Eldik, G J; Ruiter, R K; Colla, P H; van Herpen, M M; Schrauwen, J A; Wullems, G J

    1997-03-01

    Successful sexual reproduction relies on gene products delivered by the pistil to create an environment suitable for pollen tube growth. These compounds are either produced before pollination or formed during the interactions between pistil and pollen tubes. Here we describe the pollination-enhanced expression of the cp100 gene in pistils of Solanum tuberosum. Temporal analysis of gene expression revealed an enhanced expression already one hour after pollination and lasts more than 72 h. Increase in expression also occurred after touching the stigma and was not restricted to the site of touch but spread into the style. The predicted CP100 protein shows similarity to leguminous isoflavone reductases (IFRs), but belongs to a family of IFR-like NAD(P)H-dependent oxidoreductases present in various plant species. PMID:9106515

  1. Enhanced production of antimicrobial sesquiterpenes and lipoxygenase metabolites in elicitor-treated hairy root cultures of Solanum tuberosum.

    Science.gov (United States)

    Komaraiah, P; Reddy, G V; Reddy, P Srinivas; Raghavendra, A S; Ramakrishna, S V; Reddanna, P

    2003-04-01

    Potato (Solanum tuberosum) hairy root cultures, established by infecting potato tuber discs with Agrobacterium rhizogenes, were used as a model system for the production of antimicrobial sesquiterpenes and lipoxygenase (LOX) metabolites. Of the four sesquiterpene phytoalexins (rishitin, lubimin, phytuberin and phytuberol) detected in elicitor-treated hairy root cultures, rishitin (213 micrograms g-1 dry wt) was the most predominant followed by lubimin (171 micrograms g-1 dry wt). The elicitors also induced LOX activity (25-fold increase) and LOX metabolites, mainly 9-hydroxyoctadecadienoic acid and 9-hydroxyoctadecatrienoic acid, in potato hairy root cultures. The combination of fungal elicitor plus cyclodextrin was the most effective elicitor treatment, followed by methyl jasmonate plus cyclodextrin in inducing sesquiterpenes and LOX metabolites. PMID:12882150

  2. Caracterización de zonas sensibles a heladas en el cultivo de papa (Solanum Tuberosum) en Boyacá

    OpenAIRE

    Álvaro Enrique Alvarado-Gaona; Pablo Antonio Serrano-Cely; Carmenza Pérez-F.

    2011-01-01

    La papa (Solanum tuberosum), como todas las plantas cultivadas, depende de factores atmosféricos; por tanto, las heladas se convierten en un factor limitante para su producción, lo que ha ocasionado que los agricultores opten por cultivar en zonas con alturas superiores a 3.000 msnm, para ampliar la frontera agrícola. En estos sitios, las heladas son más severas, además del daño irreversible a los ecosistemas estratégicos como los páramos ya que la aplicación excesiva de agroquímicos y el sis...

  3. Isolation and characterization of a Solanum tuberosum subtilisin-like protein with caspase-3 activity (StSBTc-3).

    Science.gov (United States)

    Fernández, María Belén; Daleo, Gustavo Raúl; Guevara, María Gabriela

    2015-01-01

    Plant proteases with caspase-like enzymatic activity have been widely studied during the last decade. Previously, we have reported the presence and induction of caspase-3 like activity in the apoplast of potato leaves during Solanum tuberosum- Phytophthora infestans interaction. In this work we have purified and identified a potato extracellular protease with caspase-3 like enzymatic activity from potato leaves infected with P. infestans. Results obtained from the size exclusion chromatography show that the isolated protease is a monomeric enzyme with an estimated molecular weight of 70 kDa approximately. Purified protease was analyzed by MALDI-TOF MS, showing a 100% of sequence identity with the deduced amino acid sequence of a putative subtilisin-like protease from S. tuberosum (Solgenomics protein ID: PGSC0003DMP400018521). For this reason the isolated protease was named as StSBTc-3. This report constitutes the first evidence of isolation and identification of a plant subtilisin-like protease with caspase-3 like enzymatic activity. In order to elucidate the possible function of StSBTc-3 during plant pathogen interaction, we demonstrate that like animal caspase-3, StSBTc-3 is able to produce in vitro cytoplasm shrinkage in plant cells and to induce plant cell death. This result suggest that, StSBTc-3 could exert a caspase executer function during potato- P. infestans interaction, resulting in the restriction of the pathogen spread during plant-pathogen interaction. PMID:25486023

  4. Effects of haulm killing and gibberellic acid on seed potato (Solanum tuberosum L.) and techniques for micro- and minituber production in northern latitudes

    OpenAIRE

    Virtanen, E.

    2014-01-01

    Abstract Seed potato is the starting point in the potato (Solanum tuberosum L.) production chain. In order to secure potato production in a variety of production conditions, plant diseases must be controlled and the yield characteristics of the used cultivars ensured. In addition, production must be cost-effective. Characteristics particular to northern production conditions include long periods of daylight and a short growing season as well as a several months long seed potato storage pe...

  5. Optimasi Deteksi Dini Potato Virus Y (PVY) Pada Kentang Solanum tuberosum L. varietas Kalosi Dengan Teknik ELISA (Enzyme Linked Immunosorbent Assay)

    OpenAIRE

    Zainuddin, Masniawati; Kuswinanti, Tutik; Gobel, Risco B.; Toyyibah, Indah

    2011-01-01

    A research about Optimization of Early Detection of Potato Virus Y (PVY) in Potato Solanum tuberosum L. varieties Kalosi by ELISA Technique (Enzyme linked immunosorbent assay) was done. The purpose of this research was to determine the level of sensitivity of the combination of antibody and antigen dilutions with ELISA technique in detecting the virus PVY in potato plants. The research was carried out in the Laboratory of Agricultural Biotechnology, Research Center of Hasanuddin University, M...

  6. SNPs in Genes Functional in Starch-Sugar Interconversion Associate with Natural Variation of Tuber Starch and Sugar Content of Potato (Solanum tuberosum L.)

    OpenAIRE

    Schreiber, Lena; Nader-Nieto, Anna Camila; Schönhals, Elske Maria; Walkemeier, Birgit; Gebhardt, Christiane

    2014-01-01

    Starch accumulation and breakdown are vital processes in plant storage organs such as seeds, roots, and tubers. In tubers of potato (Solanum tuberosum L.) a small fraction of starch is converted into the reducing sugars glucose and fructose. Reducing sugars accumulate in response to cold temperatures. Even small quantities of reducing sugars affect negatively the quality of processed products such as chips and French fries. Tuber starch and sugar content are inversely correlated complex trait...

  7. Nematofauna asociada a la rizosfera de papas (Solanum tuberosum) cultivadas en la zona productora del Cofre de Perote, Veracruz, México

    OpenAIRE

    Damaris Desgarennes; Petra Sánchez-Nava; Reyes Peña-Santiago; Gloria Carrión

    2009-01-01

    Se determinaron y clasificaron en grupos tróficos las especies de nematodos asociados a la rizosfera de Solanum tuberosum en suelos altamente infectados por el nematodo dorado de la papa (Globodera rostochiensis) en un ciclo de cultivo, en la zona productora del Cofre de Perote, Veracruz, México. Se identificaron 7 géneros (Aphelenchoides, Aphelenchus, Crassolabium, Mesodorylaimus, Plectus y Steinernema) y 8 especies (Aporcelaimellus obtusicaudatus, Ecumenicus monohystera, Acrobeles mariannae...

  8. Effect of different growth media on number of minitubers and concentration of elements in potato (Solanum tuberosum L) shoot and root

    OpenAIRE

    M. Hajiaghaei Kmrani; K. Hashemimajd; N.Najafi; S. J. Tabatabaei

    2012-01-01

    Potato (Solanum tuberosum L.) is one of the most important economical vegetable. Minitubers are small seed tubers without pathogens that are produced from invitro plantlets after translocation to greenhouse and create healthy and high quality seed tubers. Hydroponic systems increase the production of virus-free potato minitubers. This research was carried out to study the effect of different growth media on number of minitubers and concentration of elements in potato shoot and root and also t...

  9. Development of a real-time PCR method for the differential detection and quantification of four solanaceae in GMO analysis: potato (Solanum tuberosum), tomato (Solanum lycopersicum), eggplant (Solanum melongena), and pepper (Capsicum annuum).

    Science.gov (United States)

    Chaouachi, Maher; El Malki, Redouane; Berard, Aurélie; Romaniuk, Marcel; Laval, Valérie; Brunel, Dominique; Bertheau, Yves

    2008-03-26

    The labeling of products containing genetically modified organisms (GMO) is linked to their quantification since a threshold for the presence of fortuitous GMOs in food has been established. This threshold is calculated from a combination of two absolute quantification values: one for the specific GMO target and the second for an endogenous reference gene specific to the taxon. Thus, the development of reliable methods to quantify GMOs using endogenous reference genes in complex matrixes such as food and feed is needed. Plant identification can be difficult in the case of closely related taxa, which moreover are subject to introgression events. Based on the homology of beta-fructosidase sequences obtained from public databases, two couples of consensus primers were designed for the detection, quantification, and differentiation of four Solanaceae: potato (Solanum tuberosum), tomato (Solanum lycopersicum), pepper (Capsicum annuum), and eggplant (Solanum melongena). Sequence variability was studied first using lines and cultivars (intraspecies sequence variability), then using taxa involved in gene introgressions, and finally, using taxonomically close taxa (interspecies sequence variability). This study allowed us to design four highly specific TaqMan-MGB probes. A duplex real time PCR assay was developed for simultaneous quantification of tomato and potato. For eggplant and pepper, only simplex real time PCR tests were developed. The results demonstrated the high specificity and sensitivity of the assays. We therefore conclude that beta-fructosidase can be used as an endogenous reference gene for GMO analysis. PMID:18303841

  10. Natural co-infection of Solanum tuberosum crops by the Potato yellow vein virus and potyvirus in Colombia

    Directory of Open Access Journals (Sweden)

    Angela Villamil-Garzón

    2014-08-01

    Full Text Available The Potato yellow vein virus (PYVV, a Crinivirus with an RNA tripartite genome, is the causal agent of the potato yellow vein disease, reported in Colombian since 1950, with yield reductions of up to 50%. Co-infection of two or more viruses is common in nature and can be associated with differences in virus accumulation and symptom expression. No evidence of mixed infection between PYVV and other viruses has been reported. In this study, eight plants showing yellowing PYVV symptoms: four Solanum tuberosum Group Phureja (P and four Group Andigena (A, were collected in Cundinamarca, Colombia to detect mixed infection in the isolates using next generation sequencing (NGS. The Potato virus Y (PVY complete genome (similar to N strain and the Potato virus V (PVV partial genomes were detected using NGS and re-confirmed by RT-PCR. Preliminary field screening in a large sample showed that PYVV and PVY co-infect potato plants with a prevalence of 21% within the P group and 23% within the A group. This is the first report of co-infection of PYVV and potyvirus in Colombia and with the use of NGS. Considering that potyviruses enhance symptom severity and/or yield reductions in mixed infections, our results may be relevant for disease diagnosis, breeding programs and tuber certification.

  11. Effect of irradiance, sucrose, and CO2 concentration on the growth of potato (Solanum tuberosum L.) in vitro

    Science.gov (United States)

    Yorio, Neil C.; Wheeler, Raymond M.; Weigel, Russell C.

    1995-01-01

    Growth measurements were taken of potato plantlets (Solanum tuberosum L.) cvs. Norland (NL), Denali (DN), and Kennebec (KN), grown in vitro. Studies were conducted in a growth chamber, with nodal explants grown for 21 days on Murashige and Skoog salts with either 0, 1, 2, or 3% sucrose and capped with loose-fitted Magenta 2-way caps that allowed approximately 2.25 air exchanges/hour. Plantlets were exposed to either 100 or 300 micro mol/sq m/s photosynthetic photon flux (PPF), and the growth chamber was maintained at either 400 or 4000 micro mol/mol CO2. Regardless of PPF, all cvs. that were grown at 4000 micro mol/mol CO2 showed significant increases in total plantlet dry weight (TDW) and shoot length (SL) when sucrose was omitted from the media, indicating an autotrophic response. At 400 micro mol/mol CO2, all cvs. showed an increase in TDW and SL with increasing sucrose under both PPF levels. Within any sucrose treatment, the highest TDW for all cvs. resulted from 300 micro mol/sq m/s PPF and 4000 micro mol/mol CO2 At 4000 micro mol/mol CO2, TDW showed no further increase with sucrose levels above 1% for cvs. NL and DN at both PPF levels, suggesting that sucrose levels greater than 1% may hinder growth when CO2 enrichment is used.

  12. Isolation and Purification of a Novel Deca-Antifungal Peptide from Potato (Solanum tuberosum L. cv. Jopung Against Candida albicans

    Directory of Open Access Journals (Sweden)

    Yoonkyung Park

    2012-03-01

    Full Text Available In a previous study, an antifungal protein, AFP-J, was purified from tubers of the potato (Solanum tuberosum cv. L Jopung and by gel filtration and HPLC. In this study, the functional peptide was characterized by partial acid digestion using HCl and HPLC. We obtained three peaks from the AFP-J, the first and third peaks were not active in the tested fungal strain. However, the second peak, which was named Potide-J, was active (MIC; 6.25 μg/mL against Candida albicans. The amino acid sequences were analyzed by automated Edman degradation, and the amino acid sequence of Potide-J was determined to be Ala-Val-Cys-Glu-Asn-Asp-Leu-Asn-Cys-Cys. Mass spectrometry showed that its molecular mass was 1083.1 Da. Finally, we confirmed that a disulfide bond was present between Cys3 and Cys9 or Cys10. Using this structure, Potide-J was synthesized via solid-phase methods. In these experiments, only the linear sequence was shown to display strong activity against Candida albicans. These results suggest that Potide-J may be an excellent candidate compound for the development of commercially applicable antibiotic agents.

  13. Characterization and Transcriptional Profile of Genes Involved in Glycoalkaloid Biosynthesis in New Varieties of Solanum tuberosum L.

    Science.gov (United States)

    Mariot, Roberta Fogliatto; de Oliveira, Luisa Abruzzi; Voorhuijzen, Marleen M; Staats, Martijn; Hutten, Ronald C B; van Dijk, Jeroen P; Kok, Esther J; Frazzon, Jeverson

    2016-02-01

    Before commercial release, new potato (Solanum tuberosum) varieties must be evaluated for content of toxic compounds such as glycoalkaloids (GAs), which are potent poisons. GA biosynthesis proceeds via the cholesterol pathway to α-chaconine and α-solanine. The goal of this study was to evaluate the relationship between total glycoalkaloid (TGA) content and the expression of GAME, SGT1, and SGT3 genes in potato tubers. TGA content was measured by HPLC-MS, and reverse transcription quantitative polymerase chain reactions were performed to determine the relative expression of GAME, SGT1, and SGT3 genes. We searched for cis-elements of the transcription start site using the PlantPAN database. There was a relationship between TGA content and the relative expression of GAME, SGT1, and SGT3 genes in potato tubers. Putative promoter regions showed the presence of several cis-elements related to biotic and abiotic stresses and light. These findings provide an important step toward understanding TGA regulation and variation in potato tubers. PMID:26768994

  14. Nematofauna asociada a la rizosfera de papas (Solanum tuberosum) cultivadas en la zona productora del Cofre de Perote, Veracruz, México Nematode fauna associated with the rhizosphere of potato crop (Solanum tuberosum) grown in the region of Cofre de Perote, Veracruz, Mexico

    OpenAIRE

    Damaris Desgarennes; Petra Sánchez-Nava; Reyes Peña-Santiago; Gloria Carrión

    2009-01-01

    Se determinaron y clasificaron en grupos tróficos las especies de nematodos asociados a la rizosfera de Solanum tuberosum en suelos altamente infectados por el nematodo dorado de la papa (Globodera rostochiensis) en un ciclo de cultivo, en la zona productora del Cofre de Perote, Veracruz, México. Se identificaron 7 géneros (Aphelenchoides, Aphelenchus, Crassolabium, Mesodorylaimus, Plectus y Steinernema) y 8 especies (Aporcelaimellus obtusicaudatus, Ecumenicus monohystera, Acrobeles mariannae...

  15. El manganeso como factor positivo en la producción de papa (solanum tuberosum l.) y arveja (pisum sativum l.) en suelos del altiplano cundiboyacense

    OpenAIRE

    Gómez, Manuel Iván; López, Miguel Ángel; Cifuentes, Yenny Carolina

    2010-01-01

    En dos suelos contrastantes, Andic Eutrudepts (Funza, Cundinamarca) y Typic Hapludands (Saboyá, Boyacá), se evaluó la respuesta agronómica a la aplicación de manganeso de Solanum tuberosum L. y Pisum sativum L. En S. tuberosum se realizó la investigación en dos localidades, Funza y Saboyá, evaluando dosis de Mn de 0,0; 3,0; 4,0; 6,0 kg· ha-1, respectivamente, aplicado en mezcla con el fertilizante NPK en siembra; en Saboyá se contó con un testigo sin aplicación de fertilizante. Las variedades...

  16. Distribución Diferencial de Bacterias con Potencial Biocontrolador de Spongospora subterranea en Plantas de Papa (Solanum tuberosum cv. Diacol Capiro Differential Distrubution of Candidadate Biocontrol Bacteria against Spongospora subterranea in Potato Plants (Solanum tuberosum cv. Diacol Capiro

    Directory of Open Access Journals (Sweden)

    Juliana Soler Arango

    2012-06-01

    Full Text Available Resumen. La búsqueda de microorganismos promisorios para biocontrol de patógenos de plantas en el suelo con frecuencia se ha orientado a la detección de productores de hormonas y enzimas líticas, condiciones asociadas a su capacidad para promover crecimiento vegetal. Sin embargo, el suelo es altamente variable en escalas pequeñas y se conoce poco sobre la distribución espacial de microorganismos que expresan esas funciones. Este estudio determinó la capacidad de producción de indoles totales y quitinasas en bacterias cultivables aisladas del interior de la raíz, la rizósfera, la superficie de los tubérculos o suelo de un cultivo de papa (Solanum tuberosum variedad Diacol Capiro. Las muestras se obtuvieron en un campo comercial en el municipio de la Unión en el departamento de Antioquia, Colombia, en plantas que presentaban síntomas de infección por Spongospora subterranea. Se encontró una producción diferencial de indoles o quitinasas según el sitio de aislamiento de las bacterias. No se encontró una alta producción de ambas actividades en un sólo aislamiento, la mayor producción de indoles totales y quitinasas se encontró en bacterias aisladas a partir de raíz y rizósfera, comparada con las aisladas de tubérculos y suelo. Estos resultados sugieren que en el suelo no es aleatoria la distribución de funciones deseables en controladores biológicos. Con estos resultados es posible orientar su búsqueda para optimizar el uso de recursos y el desarrollo de nuevos bioproductos.Abstract. Searching for promising microorganisms to biocontrol soil-borne plant pathogens has been often focused on producers of hormone and lytic enzymes, since these traits are linked to plant growth promotion. However, the environment from inner roots to bulk soil is quite variable, and little is known about the spatial distribution of microorganisms expressing these desirable traits. Using potato plants (Solanum tuberosum var. Diacol Capiro from a

  17. ACUMULACIÓN Y DISTRIBUCIÓN DE MATERIA SECA DE CUATRO VARIEDADES DE PAPA (Solanum tuberosum L. EN ZIPAQUIRÁ, CUNDINAMARCA (COLOMBIA DRY MATTER ALLOCATION AND PARTITIONING OF FOUR POTATO VARIETIES (Solanum tuberosum L. IN ZIPAQUIRÁ, CUNDINAMARCA (COLOMBIA

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Ñústez López

    2009-06-01

    Full Text Available Esta investigación evaluó la acumulación y distribución de materia seca en los diferentes órganos de la planta de cuatro variedades de papa (Solanum tuberosum L. en el municipio de Zipaquirá (Cundinamarca uno de los mayores productores de papa en Colombia. Se evaluaron las variedades Betina, Pastusa Suprema y Esmeralda (desarrolladas por el programa de mejoramiento genético de papa de la Facultad de Agronomía de la Universidad Nacional de Colombia y la variedad Diacol Capiro, que es una de las más sembradas en el país. Se determinó la materia seca de cada uno de los órganos de la planta por medio de muestreos destructivos de tres sitios de siembra al azar por variedad, realizados cada dos semanas durante el ciclo de cultivo. Se observaron diferencias en cuanto al comportamiento de acumulación de materia seca en hojas y tallos, siendo mayor para Pastusa Suprema a las 16 semanas después de emergencia (sde. Betina y Esmeralda acumularon la mayor parte de la materia seca de los tubérculos en la etapa final del ciclo de cultivo, entre las 16 y 18 sde, mientras que Diacol Capiro y Pastusa Suprema acumularon materia seca en estos órganos en forma progresiva a lo largo del ciclo de cultivo. La variedad Pastusa Suprema presentó el menor Índice de Cosecha con 72% siendo inferior a las variedades Diacol Capiro, Esmeralda y Betina. Debido a esta alta partición de materia seca hacia los tubérculos en la etapa final del ciclo, no se recomienda la práctica de "agobio" o "corte de rama", ya que puede disminuir el rendimiento en estas variedades.This investigation evaluated the dry matter production and partitioning of four potato varieties (Solanum tuberosum L. in the Zipaquirá town (Cundinamarca Department one of the biggest production zones of potato in Colombia. Betina, Pastusa Suprema and Esmeralda (varieties developed by the program of genetic improvement of potato of the Faculty of Agronomy of the Universidad Nacional de Colombia and

  18. Efecto del tratamiento térmico sobre diferentes parámetros de calidad de la patata (Solanum tuberosum): textura y almidón

    OpenAIRE

    Muñoz Martin, Sergi

    2012-01-01

    La patata (Solanum tuberosum) tiene una elevada biodiversidad y un alto contenido de almidón que le permite ocupar el cuarto lugar mundial en importancia como alimento. Su forma de consumo más habitual es cocida, hervida o frita, pero la composición inicial de la patata, el tratamiento realizado y la intensidad del mismo pueden tener diferente repercusión sobre la calidad final de la patata, y por lo tanto, sobre su aceptación a la hora del consumo. El objetivo del presente tra...

  19. Detección y cuantificación del Potato yellow vein virus (PYVV) en aislamientos de diferentes órganos de Solanum tuberosum Grupo Phureja

    OpenAIRE

    Hernández Guzmán, Anngie Katherine

    2012-01-01

    Potato vein yellow virus (PYVV) es un virus reemergente que infecta a la especie Solanum tuberosum en algunos países andinos causando pérdidas importantes en la producción. El virus ha sido detectado por RT-PCR convencional y en sólo dos ocasiones se ha reportado el uso de PCR en tiempo real para el diagnóstico del virus. No existen estudios sobre la distribución y acumulación de PYVV en diferentes órganos de una planta infectada, por lo tanto no se sabe si la distribución del virus en planta...

  20. A maternally inherited DNA marker, descended from Solanum demissum (2n = 6x = 72) to S. tuberosum (2n = 4x = 48)

    OpenAIRE

    Sanetomo, Rena; Hosaka, Kazuyoshi

    2011-01-01

    A Mexican hexaploid wild potato species, Solanum demissum (dms), was only used as a female in previous breeding programs. The resulting clones with dms cytoplasm produced abundant, but non-functional pollen. A 170 bp DNA fragment, named Band 1, was originally detected in the F1 hybrid between dms and S. tuberosum. In this study, the sequenced region was extended to 1,032 bp; nevertheless, it did not show any homology to known sequences. This extended region harboring Band 1 was, without intro...

  1. Génomique comparative des bactéries Dickeya solani et Pectobacterium wasabiae, pathogènes émergents chez Solanum tuberosum

    OpenAIRE

    Khayi, Slimane

    2015-01-01

    Les bactéries pectinolytiques appartenant aux genres Pectobacterium et Dickeya spp. sont des agents pathogènes chez Solanum tuberosum. Ces bactéries sont responsables de la maladie de la jambe noire et de la pourriture molle lors de la culture et du stockage des tubercules. Ce travail de thèse est divisé en deux axes: 1) Etude dela diversité d'une population du pathogène D. solani par approche de génomique comparée afin de mieux comprendre la structure génomique de cette espèce émergente. 2) ...

  2. Selection of anthocyanin-accumulating potato (Solanum tuberosum L.) cell lines from calli derived from seedlings produced by gamma-irradiated seeds

    International Nuclear Information System (INIS)

    Callus cell lines of potato (Solanum tuberosum L. cv. Zarevo) were obtained from seedlings germinated from gamma-irradiated seeds (200 Gy). Some of these cell lines produce red-violet pigments which were identified as acylated anthocyanins. The major anthocyanin was determined to be peonidin 3-O-[6-O-(4-O-E-p-coumaroyl-rhamnosyl)-glucoside]-5-O-glucoside (“peonanin”). Single cell-derived protoclones from non-pigmented protoplasts sometimes also gave rise to pigmented cell clusters thus indicating that the changes in the expression of the anthocyanin pathway can also occur after the stage of initial callus induction

  3. Detección de Ca Liberibacter solanacearum y fitoplasmas en cultivo de papa (Solanum tuberosum L.) en el Valle de Toluca

    OpenAIRE

    Ana Tarin Gutiérrez-Ibáñez; Jesús Ricardo Sánchez Pale; Antonio Laguna Cerda; José Francisco Ramírez Dávila; Artemio Balbuena Melgarejo; Omar Guadalupe Alvarado Gómez

    2013-01-01

    Título en ingles: Detection of Ca Liberibacter solanacearum and phytoplasma in potato crop (Solanum tuberosum L.) in Toluca Valley Título corto: Detección de Ca Liberibacter solanacearum y fitoplasmas Resumen En México y Centro América se han detectado tubérculos de papa con manchado interno. Recientemente en Texas EUA a esta enfermedad se le ha denominado “Zebra Chip” (ZC) o rayado de la papa, los síntomas foliares se asemejan al síndrome denominado “Punta Morada de la Papa” (PMP) o enfermed...

  4. Detección de ca liberibacter solanacearum y fitoplasmas en cultivo de papa (solanum tuberosum l.) en el valle de toluca

    OpenAIRE

    Gutiérrez-Ibáñez, Ana Tarin; Jesús Ricardo SÁNCHEZ PALE; Laguna Cerda, Antonio; Ramírez Dávila, José Francisco; Balbuena Melgarejo, Artemio; ALVARADO GÓMEZ, OMAR GUADALUPE

    2013-01-01

    Título en ingles: Detection of Ca Liberibacter solanacearum and phytoplasma in potato crop (Solanum tuberosum L.) in Toluca Valley Título corto: Detección de Ca Liberibacter solanacearum y fitoplasmas Resumen En México y Centro América se han detectado tubérculos de papa con manchado interno. Recientemente en Texas EUA a esta enfermedad se le ha denominado “Zebra Chip” (ZC) o rayado de la papa, los síntomas foliares se asemejan al síndrome denominado “Punta Morada de la Papa” (PMP) o enfermed...

  5. Purification and some properties of pectinesterase from potato (Solanum tuberosum L. alpha cultivar

    Directory of Open Access Journals (Sweden)

    Julio Montañez Sáenz

    2000-01-01

    Full Text Available Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel.A pectinesterase foi extraída da batata (cultivar do alfa, purificada e parcialmente caracterizada. O protocolo usado levou a uma proteína purificada 58,8 vezes (51 850,2 units/mg da proteína com uma recuperação de 15,5 % da atividade da proteína. A enzima purificada apresentou um peso molecular de 27 kDa e seu ponto isoelétrico foi ao redor 4,5. A pectinesterase exibiu pH e temperatura ótimos de respectivamente 8,0 e 60°C. A enzima purificada apresentou um único pico simétrico de atividade específica após as etapas de cromatografia. A homogeneidade da pectinesterase purificada foi confirmada por filtração em gel e por eletroforese em gel de poliacrilamida.

  6. Results regarding new romanian potato (Solanum tuberosum L. cultivars reaction to in vitro culture conditions

    Directory of Open Access Journals (Sweden)

    Anca BACIU

    2009-11-01

    Full Text Available The Solanum genus presents a great importance for research due to its economical importance being a great aim to different breeding programs. It is the best represented genus from the Solanaceae family, part of this family species producing tubers. Working method used in our experimentations was double node fragments culture. Our studies aimed regeneration and multiplication of four potato cultivars (Desirée, Redsec, Ts. 95-1161-66 and Ts. 94-1117-98 in order to improve the multiplication and pathogen free material obtaining protocols. Biometrical determinations performed for explants and new plantlets made possible to conclude as the best in vitro response was given by the cultivar Redsec both regarding the regeneration, growth and multiplication capacity and in foliar surface evolution, overcoming even the control (Desiree variety and the cultivar Redsec can be recommended for other in vitro experimentations being a potent cultivar for this type of culture.

  7. Effect of Potato (Solanum tuberosum L.) Cropping Systems on Soil and Nutrient Losses Through Runoff in a Humic Nitisol, Kenya

    Science.gov (United States)

    Nyawade, Shadrack; Charles, Gachene; Karanja, Nancy; Elmar, Schulte-Geldermann

    2016-04-01

    Soil erosion has been identified as one of the major causes of soil productivity decline in the potato growing areas of East African Highlands. Potato establishes a protective soil cover only at about 45-60 days after planting and does not yield sufficient surface mulch upon harvest which leaves the soil bare at the critical times when rainfall intensities are usually high thus exposes soil to erosion. A field study was carried out using runoff plots during the short and long rainy seasons of 2014/15 respectively at the University of Nairobi Upper Kabete Farm, Kenya. The objectives were to assess the effect of soil surface roughness and potato cropping systems on soil loss and runoff, to determine the effect of erosion on nutrient enrichment ratio and to evaluate the soil organic matter fraction most susceptible to soil erosion. The treatments comprised of Bare Soil (T1); Potato + Garden Pea (Pisum sativa) (T2); Potato + Climbing Bean (Phaseolus vulgaris) (T3); Potato + Dolichos (Lablab purpureus) (T4) and Sole Potato (Solanum tuberosum L.) (T5). The amount of soil loss and runoff recorded in each event differed significantly between treatments (pMean cumulative soil loss reduced by 6.4, 13.3 and 24.4 t ha-1from T2, T3 and T4 respectively compared to sole potato plots (T5), while mean cumulative runoff reduced by 8.5, 17.1 and 28.3 mm from T2, T3 and T4 respectively when compared with the sole potato plots (T5) indicating that T4 plots provided the most effective cover in reducing soil loss and runoff. Regression analyses revealed that both runoff and soil loss related significantly with surface roughness and percent cover (R2=0.83 and 0.73 respectively, pInternational Agricultural Research for Development Fund.

  8. Hyperspectral remote sensing for advanced detection of early blight (Alternaria solani) disease in potato (Solanum tuberosum) plants

    Science.gov (United States)

    Atherton, Daniel

    Early detection of disease and insect infestation within crops and precise application of pesticides can help reduce potential production losses, reduce environmental risk, and reduce the cost of farming. The goal of this study was the advanced detection of early blight (Alternaria solani) in potato (Solanum tuberosum) plants using hyperspectral remote sensing data captured with a handheld spectroradiometer. Hyperspectral reflectance spectra were captured 10 times over five weeks from plants grown to the vegetative and tuber bulking growth stages. The spectra were analyzed using principal component analysis (PCA), spectral change (ratio) analysis, partial least squares (PLS), cluster analysis, and vegetative indices. PCA successfully distinguished more heavily diseased plants from healthy and minimally diseased plants using two principal components. Spectral change (ratio) analysis provided wavelengths (490-510, 640, 665-670, 690, 740-750, and 935 nm) most sensitive to early blight infection followed by ANOVA results indicating a highly significant difference (p < 0.0001) between disease rating group means. In the majority of the experiments, comparisons of diseased plants with healthy plants using Fisher's LSD revealed more heavily diseased plants were significantly different from healthy plants. PLS analysis demonstrated the feasibility of detecting early blight infected plants, finding four optimal factors for raw spectra with the predictor variation explained ranging from 93.4% to 94.6% and the response variation explained ranging from 42.7% to 64.7%. Cluster analysis successfully distinguished healthy plants from all diseased plants except for the most mildly diseased plants, showing clustering analysis was an effective method for detection of early blight. Analysis of the reflectance spectra using the simple ratio (SR) and the normalized difference vegetative index (NDVI) was effective at differentiating all diseased plants from healthy plants, except for the

  9. Fibrin(ogen)olytic and antiplatelet activities of a subtilisin-like protease from Solanum tuberosum (StSBTc-3).

    Science.gov (United States)

    Pepe, Alfonso; Frey, María Eugenia; Muñoz, Fernando; Fernández, María Belén; Pedraza, Anabela; Galbán, Gustavo; García, Diana Noemí; Daleo, Gustavo Raúl; Guevara, María Gabriela

    2016-06-01

    Plant serine proteases have been widely used in food science and technology as well as in medicine. In this sense, several plant serine proteases have been proposed as potential anti-coagulants and anti-platelet agents. Previously, we have reported the purification and identification of a plant serine protease from Solanum tuberosum leaves. This potato enzyme, named as StSBTc-3, has a molecular weight of 72 kDa and it was characterized as a subtilisin like protease. In this work we determine and characterize the biochemical and medicinal properties of StSBTc-3. Results obtained show that, like the reported to other plant serine proteases, StSBTc-3 is able to degrade all chains of human fibrinogen and to produces fibrin clot lysis in a dose dependent manner. The enzyme efficiently hydrolyzes β subunit followed by partially hydrolyzed α and γ subunits of human fibrinogen. Assays performed to determine StSBTc-3 substrate specificity using oxidized insulin β-chain as substrate, show seven cleavage sites: Asn3-Gln4; Cys7-Gly8; Glu13-Ala14; Leu15-Tyr16; Tyr16-Leu17; Arg22-Gly23 and Phe25-Tyr26, all of them were previously reported for other serine proteases with fibrinogenolytic activity. The maximum StSBTc-3 fibrinogenolytic activity was determined at pH 8.0 and at 37 C. Additionally, we demonstrate that StSBTc-3 is able to inhibit platelet aggregation and is unable to exert cytotoxic activity on human erythrocytes in vitro at all concentrations assayed. These results suggest that StSBTc-3 could be evaluated as a new agent to be used in the treatment of thromboembolic disorders such as strokes, pulmonary embolism and deep vein thrombosis. PMID:27039890

  10. Wounding, insect chewing and phloem sap feeding differentially alter the leaf proteome of potato, Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Duceppe Marc-Olivier

    2012-12-01

    Full Text Available Abstract Background Various factors shape the response of plants to herbivorous insects, including wounding patterns, specific chemical effectors and feeding habits of the attacking herbivore. Here we performed a comparative proteomic analysis of the plant's response to wounding and herbivory, using as a model potato plants (Solanum tuberosum L. subjected to mechanical wounding, defoliation by the Colorado potato beetle Leptinotarsa decemlineata Say, or phloem sap feeding by the potato aphid Macrosiphum euphorbiae Thomas. Results Out of ~500 leaf proteins monitored by two-dimensional gel electrophoresis (2-DE, 31 were up- or downregulated by at least one stress treatment compared to healthy control plants. Of these proteins, 29 were regulated by beetle chewing, 8 by wounding and 8 by aphid feeding. Some proteins were up- or downregulated by two different treatments, while others showed diverging expression patterns in response to different treatments. A number of modulated proteins identified by mass spectrometry were typical defense proteins, including wound-inducible protease inhibitors and pathogenesis-related proteins. Proteins involved in photosynthesis were also modulated, notably by potato beetle feeding inducing a strong decrease of some photosystem I proteins. Quantitative RT PCR assays were performed with nucleotide primers for photosynthesis-related proteins to assess the impact of wounding and herbivory at the gene level. Whereas different, sometimes divergent, responses were observed at the proteome level in response to wounding and potato beetle feeding, downregulating effects were systematically observed for both treatments at the transcriptional level. Conclusions These observations illustrate the differential impacts of wounding and insect herbivory on defense- and photosynthesis-related components of the potato leaf proteome, likely associated with the perception of distinct physical and chemical cues in planta.

  11. Suberin of potato (Solanum tuberosum var. Nikola): comparison of the effect of cutinase CcCut1 with chemical depolymerization.

    Science.gov (United States)

    Järvinen, Riikka; Silvestre, Armando J D; Holopainen, Ulla; Kaimainen, Mika; Nyyssölä, Antti; Gil, Ana M; Pascoal Neto, Carlos; Lehtinen, Pekka; Buchert, Johanna; Kallio, Heikki

    2009-10-14

    Chemical and enzymatic depolymerizations of suberin isolated from potato peel ( Solanum tuberosum var. Nikola) were performed under various conditions. Enzymatic hydrolysis with cutinase CcCut1 and chemical methanolysis with NaOMe of suberin yielded monomeric fragments, which were identified as TMS derivatives with GC-MS and GC-FID. The solid, hydrolysis-resistant residues were analyzed with solid state (13)C CPMAS NMR, FT-IR, and microscopic methods. Methanolysis released more CHCl(3)-soluble material than the cutinase treatment when determined gravimetrically. Interestingly, cutinase-catalyzed hydrolysis produced higher proportions of aliphatic monomers than hydrolysis with the NaOMe procedure when analyzed by GC in the form of TMS derivatives. Monomers released by the two methods were mainly alpha,omega-dioic acids and omega-hydroxy acids, but the ratios of the detected monomers were different, at 40.0 and 32.7% for methanolysis and 64.6 and 8.2% for cutinase, respectively. Thus, cutinase CcCut1 showed higher activity toward ester bonds of alpha,omega-dioic acids than toward the bonds of omega-hydroxy acids. The most abundant monomeric compounds were octadec-9-ene-1,18-dioic acid and 18-hydroxyoctadec-9-enoic acid, which accounted for ca. 37 and 28% of all monomers, respectively. The results of the analyses of the chemical and enzymatic hydrolysis products were supported by the spectroscopic analyses with FT-IR and CPMAS (13)C NMR together with the analysis of the microstructures of the hydrolysis residues by light and confocal microscopy. PMID:19739639

  12. Potato (Solanum tuberosum L.) tuber ageing induces changes in the proteome and antioxidants associated with the sprouting pattern.

    Science.gov (United States)

    Delaplace, Pierre; Fauconnier, Marie-Laure; Sergeant, Kjell; Dierick, Jean-François; Oufir, Mouhssin; van der Wal, Froukje; America, Antoine H P; Renaut, Jenny; Hausman, Jean-François; du Jardin, Patrick

    2009-01-01

    During post-harvest storage, potato tubers age as they undergo an evolution of their physiological state influencing their sprouting pattern. In the present study, physiological and biochemical approaches were combined to provide new insights on potato (Solanum tuberosum L. cv. Désirée) tuber ageing. An increase in the physiological age index (PAI) value from 0.14 to 0.83 occurred during storage at 4 degrees C over 270 d. Using this reference frame, a proteomic approach was followed based on two-dimensional electrophoresis. In the experimental conditions of this study, a marked proteolysis of patatin occurred after the PAI reached a value of 0.6. In parallel, several glycolytic enzymes were up-regulated and cellular components influencing protein conformation and the response to stress were altered. The equilibrium between the 20S and 26S forms of the proteasome was modified, the 20S form that recycles oxidized proteins being up-regulated. Two proteins belonging to the cytoskeleton were also differentially expressed during ageing. As most of these changes are also observed in an oxidative stress context, an approach focused on antioxidant compounds and enzymes as well as oxidative damage on polyunsaturated fatty acids and proteins was conducted. All the changes observed during ageing seemed to allow the potato tubers to maintain their radical scavenging activity until the end of the storage period as no accumulation of oxidative damage was observed. These data are interpreted considering the impact of reactive oxygen species on the development and the behaviour of other plant systems undergoing ageing or senescence processes. PMID:19204031

  13. Caracterización de zonas sensibles a heladas en el cultivo de papa (Solanum Tuberosum en Boyacá

    Directory of Open Access Journals (Sweden)

    Álvaro Enrique Alvarado-Gaona

    2011-02-01

    Full Text Available La papa (Solanum tuberosum, como todas las plantas cultivadas, depende de factores atmosféricos; por tanto, las heladas se convierten en un factor limitante para su producción, lo que ha ocasionado que los agricultores opten por cultivar en zonas con alturas superiores a 3.000 msnm, para ampliar la frontera agrícola. En estos sitios, las heladas son más severas, además del daño irreversible a los ecosistemas estratégicos como los páramos ya que la aplicación excesiva de agroquímicos y el sistema de monocultivo hacen que los suelos pierdan sus propiedades y por tanto, la producción disminuye. Por esta razón, el Centro Virtual de la Cadena Agroalimentaria de la papa CEVIPAPA, en convenio con la Universidad Pedagógica y tecnológica de Colombia, UPTC desarrolló un trabajo de investigación con el propósito de reconocer áreas sensibles a heladas en el departamento de Boyacá. El trabajo se desarrolló en los municipios de Toca, Motavita, Cómbita, Úmbita,Ventaquemada, Chíquiza, Tunja, Siachoque, Soracá y Saboyá,donde se evaluó la problemática causada por las heladas en este cultivo; así mismo, se detectaron las zonas de mayor producción, identificando las veredas con mayor incidencia en cada uno de los municipios, con el fin de controlar y mitigar los efectos negativos en el cultivote dicho tubérculo.

  14. Evaluación de dos tipos de esquejes para producción de tubérculo-semilla élite en cuatro variedades de papa Solanum phureja juz et buk y Solanum tuberosum

    OpenAIRE

    Caro Muñoz Marina; Moreno Rengifo Sidney Benhur; Marquinez Casas Xavier

    2004-01-01

    Esta investigación se realizó en el Laboratorio de Cultivo de Tejidos Vegetales del Instituto de Biotecnología de la Universidad Nacional de Colombia (IBUN) y en los invernaderos de la Facultad de Agronomía, Sede Bogotá. El propósito fue evaluar una metodología para propagación
    de plántulas y producción de tubérculo semilla élite en cuatro variedades de papa: Solanum tuberosum L. ssp andígena (Diacol-Capiro, ICA-Puracé y Parda Pastusa) y Solanum phureja Juz et Buk. (“Yema de huevo”...

  15. MICORRIZACIÓN In Vitro E In Vivo DE PLÁNTULAS DE PAPA (Solanum tuberosum var. Alfa

    Directory of Open Access Journals (Sweden)

    Kalyanne Fernández

    2010-01-01

    Full Text Available Las condiciones artificiales en las que se desarrollan las plantas obtenidas por micropropagación hacen que sean más susceptibles a la sequía durante el transplante. Los hongos micorrízicos arbusculares contribuyen a la supervivencia y el crecimiento de las plantas al reducir el estrés asociado con la nutrición, las relaciones con el agua, la estructura del suelo, el pH, las sales, los metales tóxicos y los patógenos, por lo que son considerados de extrema utilidad como inoculantes durante las diferentes fases de micropropagación de los cultivos. Teniendo en cuenta estos aspectos se realizó este trabajo, con el objetivo de evaluar la factibilidad de la inoculación micorrízica durante los estadios in vitro e in vivo del proceso de micropropagación del cultivo de la papa (Solanum tuberosum L var. Alfa. Para dar respuesta al trabajo, se estudió la inoculación micorrízica sobre plantas de papa en condiciones de crecimiento in vitro, empleando diferentes medios de cultivo (MS, M y un nuevo medio N, bajo un diseño completamente aleatorizado (DCA y la inoculación de plantas de papa durante el transplante a fase adaptativa empleando tres especies del género Glomus y dos concentrados de especies de HMA (selva y desierto sobre dos sustratos diferentes, utilizando un DCA bajo arreglo bifactorial. En el estudio in vitro se obtuvieron efectos positivos sobre las plantas inoculadas en el medio M, aun cuando quedó clara la necesidad de encontrar nuevos medios, nutricionalmente balanceados, que garanticen tanto el crecimiento de las plántulas como el establecimiento eficiente de la micorrización. Además, en la fase adaptativa se encontró una respuesta altamente positiva a la inoculación de las cepas, mostrando un comportamiento diferenciado en función de los sustratos, apreciable en todas las variables estudiadas (nutricionales, fúngicas y fisiológicas, lo cual demostró la factibilidad del uso de dichos microorganismos en este

  16. Transcriptome Profiling of the Potato (Solanum tuberosum L.) Plant under Drought Stress and Water-Stimulus Conditions.

    Science.gov (United States)

    Gong, Lei; Zhang, Hongxia; Gan, Xiaoyan; Zhang, Li; Chen, Yuchao; Nie, Fengjie; Shi, Lei; Li, Miao; Guo, Zhiqian; Zhang, Guohui; Song, Yuxia

    2015-01-01

    Drought stress can seriously affect tuberization, yield and quality of potato plant. However, the precise molecular mechanisms governing potato stolon's response to drought stress and water supply are not very well understood. In this work, a potato (Solanum tuberosum L.) variant, Ningshu 4, was subjected to severe drought stress treatment (DT) and re-watering treatment (RWT) at tuber bulking stage. Strand-specific cDNA libraries of stolon materials were constructed for paired-end transcriptome sequencing analyses and differentially expressed gene (DEG) examination. In comparison to untreated-control (CT) plants, 3189 and 1797 DEGs were identified in DT and RWT plants and 4154 solely expressed DEGs were screened out from these two comparison groups. Interestingly, 263 genes showed opposite expression patterns in DT and RWT plants. Among them, genes homologous to Protein Phosphatase 2C (PP2C), Aspartic protease in guard cell 1 (ASPG1), auxin-responsive protein, Arabidopsis pseudo response regualtor 2 (APRR2), GA stimulated transcripts in Arabidopsis 6 (GASA6), Calmodulin-like protein 19 (CML19), abscisic acid 8'-hydroxylases and calcium-transporting ATPase, et al. were related with drought-stress and water stimulus response. Sixteen DEGs involved in starch synthesis, accumulation and tuber formation exhibited significantly different expression upon re-watering. In addition, 1630, 1527 and 1596 transcription factor encoding genes were detected in CT, DT and RWT. DEGs of ERF, bHLH, MYB, NAC, WRKY, C2H2, bZIP and HD-ZIP families accounted for 50% in three comparison groups, respectively. Furthermore, characteristics of 565 gene ontology (GO) and 108 Kyoto Encyclopedia of Genes and Genomes pathways (KEGG) were analyzed with the 4154 DEGs. All these results suggest that the drought- and water-stimulus response could be implemented by the regulated expression of metabolic pathway DEGs, and these genes were involved in the endogenous hormone biosynthesis and signal

  17. Effects of Potassium Mineral Fertilization on Potato (Solanum tuberosum L.) Yield on a Chernozem Soil in Hungary

    Science.gov (United States)

    László, Márton, ,, Dr.

    2010-05-01

    Nowadays potato (Solanum tuberosum L.) is a more important throughout the world between field crops. As potato is such a potassium demanding crop, it is particularly important that the potassium fertilizers used should be correctly balanced. Applying the adequate quantity of balanced K- fertilizer is the first requirement for achieving optimum yield and doing so will result in potatoes of acceptable quality. Potato potassium nutrition has been studied at the Research Institute for Soil Science and Agricultural Chemistry of the Hungarian Academy of Sciences (RISSAC-HAS)- Experiment Station, Nagyhörcsök (chernozem soil) in a long term field experiment designed to determine NPK- nutrient requirements. The method of the experiment was 43 mixed factorial design with 64 treatments in 2 repetitions with 128 plots. The residual effects of K- levels brought about by build- up fertilization were studied. Potato were planted in 1978. The experimental dates were estimated by multivariate analysis of variance (MANOVA). On the basis of foliar analysis at early flowering about 4.5 to 5.0% K in dry matter proved to be satisfactory for obtaining maximum yield (32.6 t/ha). The yields increased by 22%, 34%, and 38% at 124-140, 141-168 and 169-208 ppm soil AL- K2O rates, respectively. The tubers concentrated much N 19% and less P 81% than potassium. Results for tuber maximum uptake of potassium reached a maximum about 130 days after planting. The improvement of the K supply of the soil increased yield and induced low concentrations of numerous microelements on leaves considered to be important. Key words: Potato (Solanum tuberosum L.), chernozem soil, potassium, yield Introduction Potato is an important food crop, more particularly in the temperate zone, especially in Europe and Asia. Between 1981 and 1995, in spite of an 18% decrease in cultivated area production increased by 13% because the average yield increased from 11.0 to 15.1 t/ha. Potato is a soil nutrients demanding crop

  18. Efecto de la consistencia del medio de cultivo y del nitrato de plata en la micropropagación de dos cultivares de papa (Solanum tuberosum

    Directory of Open Access Journals (Sweden)

    Sandra Alva Ticona

    2013-12-01

    Full Text Available Título en ingles: Effect of culture medium consistence and silver nitrate on micropropagation of two potato (Solanum tuberosum cultivars Short title: Micropropagation of two potato cultivarsAbstract: Potato (Solanum tuberosum L. is one of the main crops of the Andes region in Venezuela where some S. tuberosum ssp. andigena cultivars are used, including Arbolona negra. Since 1946, the Andean cultivars have been slowly substituted with foreign potato seeds, for example cv. Granola from Germany. Potato micropropagation is an excellent alternative for the conservation of native cultivars, for the massive production of potato seeds and for the production of plantlets with adequate growth parameters that allow the study of potato-pathogen interactions. However, potato’s vitroplants frequently show symptoms caused by ethylene accumulation in the culture flasks. In this work, we compare the in vitro response of Granola and Arbolona negra cultivars using MS semi-solid or liquid medium supplemented or not with AgNO3. These potato cultivars did not show epinasty or hyperhidricity symptoms caused by ethylene when were cultivated on MS (1962 semi-solid medium supplemented with AgNO3 2 mg l-1. Under these conditions, leaf area shows the highest values for both cultivars, but there were no differences in other growth parameters such as stem length or leaf number in comparison with plantlets cultivated on medium without AgNO3. These results allowed us to recommend the use of semi-solid medium supplemented with AgNO3 for the micropropagation of these two cultivars.Key words: native potato, ethylene, vitroplantlets.Resumen: La papa (Solanum tuberosum L. es uno de los principales cultivos de la región de los Andes de Venezuela, donde algunos cultivares de S. tuberosum ssp. andigena se utilizan, entre ellos, Arbolona negra. Desde 1946, los cultivares andinos han sido poco a poco sustituidos por cultivares comerciales, por ejemplo Granola de Alemania. La

  19. The evolution of the Solanum tuberosum L. neoplantles cultivated in vitro on cuture media supplimented with bioactive magneto-fluidic nanocomposites

    Directory of Open Access Journals (Sweden)

    Anca BACIU

    2007-05-01

    Full Text Available During the last decades the bioactive magnetofluid nanocomposites proved both their unique performances and their high potential. Everywhere nanotechnologies gain ground being economic systems having a controlled period of the induced effect, of the planed biodegradation and of the used quantities. Nanotechnologies are applied in biology. They are of beneficed effects in plant and animal protection. The bioactive magnetofluid nanocomposites used for plants are applied in the vegetation phase by means of spraying. The nanocomposites are also elements of the culture media for the inducing of the calus and the regeneration of plants belonging to the species Chrysanthemum, Lilium regale, Mamillaria, Triticale and Solanum tuberosum L. TiO2 nanocrystals are used for the sterilization of the spaces where cereals are kept and for destroying the fungi from the seeds. The goal of the experiments was to test the reaction of different types of explants when cultivated in vitro on media supplemented with bioactive magnetofluid nanocomposites.

  20. Gas-exchange, water use efficiency and yield responses of elite potato (Solanum tuberosum L.) cultivars to changes in atmospheric carbon dioxide concentration, temperature and relative humidity

    DEFF Research Database (Denmark)

    Kaminski, Kacper Piotr; Sørensen, Kirsten Kørup; Nielsen, Kåre Lehmann;

    2014-01-01

    decreased stomatal conductance (25%) with a simultaneous drop in transpiration rate although not significant. The ratio Ci/Ca was in contrast to the first experiment significantly higher in plants grown at elevated [CO2]. Despite this photosynthetic acclimation, concurrent stimulation of aboveground and......In spite of the agricultural importance of potato (Solanum tuberosum L.), most plant physiology studies have not accounted for the effect of the interaction between elevated carbon dioxide concentration ([CO2]) and other consequences of climate change on WUE. In 2010, a first controlled environment...... chamber experiment (E1) was performed with two treatments: one control at a [CO2] exposure level of 380 ppm and the other at elevated [CO2] first to 700 ppm and subsequently to 1000 ppm. Plants grown at elevated [CO2] levels of 700 and 1000 ppm showed a consistent significant increase in leaf level...

  1. Evaluación del rasgo textura en tubérculos de Solanum tuberosum grupo Phureja y búsqueda de genes candidatos asociados al rasgo

    OpenAIRE

    Guateque Alba, Magda Alejandra

    2014-01-01

    Solanum tuberosum, es una especie de la familia de las solanáceas. En Colombia, es un alimento básico tanto como consumo en fresco o procesado. Sin embargo, el rasgo de textura no se ha estudiado en profundidad en los tubérculos de papa colombianos. Por tal razón en esta investigación se realizó la fenotipificación precisa de este carácter complejo y se realizó un análisis de asociación genética con marcadores moleculares tipo SNPs, con el fin de acercarse a la comprensión gené...

  2. Effect of Potato (Solanum tuberosum L.) Cropping Systems on Soil and Nutrient Losses Through Runoff in a Humic Nitisol, Kenya

    Science.gov (United States)

    Nyawade, Shadrack; Charles, Gachene; Karanja, Nancy; Elmar, Schulte-Geldermann

    2016-04-01

    Soil erosion has been identified as one of the major causes of soil productivity decline in the potato growing areas of East African Highlands. Potato establishes a protective soil cover only at about 45-60 days after planting and does not yield sufficient surface mulch upon harvest which leaves the soil bare at the critical times when rainfall intensities are usually high thus exposes soil to erosion. A field study was carried out using runoff plots during the short and long rainy seasons of 2014/15 respectively at the University of Nairobi Upper Kabete Farm, Kenya. The objectives were to assess the effect of soil surface roughness and potato cropping systems on soil loss and runoff, to determine the effect of erosion on nutrient enrichment ratio and to evaluate the soil organic matter fraction most susceptible to soil erosion. The treatments comprised of Bare Soil (T1); Potato + Garden Pea (Pisum sativa) (T2); Potato + Climbing Bean (Phaseolus vulgaris) (T3); Potato + Dolichos (Lablab purpureus) (T4) and Sole Potato (Solanum tuberosum L.) (T5). The amount of soil loss and runoff recorded in each event differed significantly between treatments (p<0.05) and were consistently highest in T1 and lowest in T4. Mean cumulative soil loss reduced by 6.4, 13.3 and 24.4 t ha-1from T2, T3 and T4 respectively compared to sole potato plots (T5), while mean cumulative runoff reduced by 8.5, 17.1 and 28.3 mm from T2, T3 and T4 respectively when compared with the sole potato plots (T5) indicating that T4 plots provided the most effective cover in reducing soil loss and runoff. Regression analyses revealed that both runoff and soil loss related significantly with surface roughness and percent cover (R2=0.83 and 0.73 respectively, p<0.05). Statistically significant linear dependence of runoff and soil loss on surface roughness and crop cover was found in T4 (p<0.05) indicating that this system was highly effective in minimizing soil loss and runoff. Enrichment ratio was on average

  3. Evaluation of NPK 8-30-20 with gypsum addition compared to fertilization employed in the region for the production of potato (Solanumtuberosum L. Evaluación de NPK 8-30-20 con yeso en comparación a la fertilización convencional para la producción de la papa (Solanum tuberosum L. Avaliação da fórmula NPK 8-30-20 com adição de gesso agrícola em comparação à adubação convencional para produção de batata (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Douglas Jobim Vieira

    2010-04-01

    best results of yield of potato tubers and better classification. The application of gypsum was important to reduce the incidence of common scab independent of the formulation and application of NPK without addition of gypsum predisposes the crop to a higher incidence of scabies.

    Diferentes formas de manejo da adubação são empregadas pelos bataticultores, sendo que, de tempos em tempos, surge uma fórmula para adubar a cultura. Porém, deve-se dar especial atenção à adubação e nutrição da cultura da batata, pois a demanda relativa de fertilizantes por unidade de área na cultura é elevada. O objetivo do trabalho foi avaliar a utilização da fórmula NPK 8-30-20 com adição de gesso agrícola comparado a adubação NPK 4-14-8 (tratamento padrão regional na produção da cultura da Batata. O trabalho foi desenvolvido na Unidade de Pesquisa em Produção de Batata e Microclima para a Agricultura, no Campus CEDETEG da Universidade Estadual do Centro Oeste – UNICENTRO em Guarapuava – PR. Os tratamentos aplicados foram: T1 - 3,5 t ha-1 de NPK 4-14-8; T2 - 3,5 t ha-1 de NPK 4-14-8 mais a aplicação de 3 t ha-1 de gesso; T3 - 1,75 t ha-1 de NPK 8-30-20; T4 - 1,75 t ha-1 de NPK 8-30-20 mais a aplicação de 3 t ha-1 de gesso; T5 - 1,75 t ha-1 de NPK 8-30-20 mais a aplicação de gesso para suprir a diferença entre os fertilizantes. O plantio da cultura da batata foi realizado no dia 23 de dezembro de 2008 e a cultivar utilizada foi a Ágata. A semente utilizada foi de Geração 01 e tubérculos de classe II. A densidade de plantio utilizada foi de 50.000 tubérculos por hectare. O delineamento experimental utilizado foi inteiramente casualizado com cinco tratamentos e quatro repetições. Avaliou-se a produtividade e a classificação dos tubérculos de acordo com o tamanho e incidência e severidade da Sarna Comum (Streptomyces spp. e Sarna

  4. Nematofauna asociada a la rizosfera de papas (Solanum tuberosum cultivadas en la zona productora del Cofre de Perote, Veracruz, México Nematode fauna associated with the rhizosphere of potato crop (Solanum tuberosum grown in the region of Cofre de Perote, Veracruz, Mexico

    Directory of Open Access Journals (Sweden)

    Damaris Desgarennes

    2009-12-01

    Full Text Available Se determinaron y clasificaron en grupos tróficos las especies de nematodos asociados a la rizosfera de Solanum tuberosum en suelos altamente infectados por el nematodo dorado de la papa (Globodera rostochiensis en un ciclo de cultivo, en la zona productora del Cofre de Perote, Veracruz, México. Se identificaron 7 géneros (Aphelenchoides, Aphelenchus, Crassolabium, Mesodorylaimus, Plectus y Steinernema y 8 especies (Aporcelaimellus obtusicaudatus, Ecumenicus monohystera, Acrobeles mariannae, Acrobeles singulus, Acrobeloides nanus, Cruznema tripartitum, Eucephalobus oxyuroides, y Globodera rostochiensis en asociación con la rizosfera de papas cultivadas. Crassolabium sp. y E. monohystera se registran por primera vez para México.Nematode species associated with the rhizosphere of Solanum tuberosum were identified and classified into trophic groups from soils highly infected by the golden potato cyst nematode (Globodera rostochiensis in a single crop cycle in the producing zone on the Cofre de Perote, Veracruz, Mexico. Seven genera (Aphelenchoides, Aphelenchus, Crassolabium, Mesodorylaimus, Plectus and Steinernema and 8 species (Aporcelaimellus obtusicaudatus, Ecumenicus monohystera, Acrobeles mariannae, Acrobeles singulus, Acrobeloides nanus, Cruznema tripartitum, Eucephalobus oxyuroides, and Globodera rostochiensis were found in association with the rhizosphere of cultivated potatoes. Crassolabium sp. and E. monohystera are recorded for the first time in México.

  5. Potatoes (Solanum tuberosum L.)

    OpenAIRE

    Dvořák, Petr; Tomášek, Jaroslav; Hamouz, Karel; Jedličková, Michaela

    2016-01-01

    In the area of potato production, targeted research solving concrete and actual problems of potato producers runs on Czech University of Life Sciences in Prague. In the last few years, we were focused on the production of new potatoes designated for early harvest, and we were focused on capitalization of yielding and qualitative characteristics of colored potato variety. These findings were further utilized and transferred to the system of organic farming. Firstly, we watched the influence of...

  6. A major QTL and an SSR marker associated with glycoalkaloid content in potato tubers from Solanum tuberosum × S. sparsipilum located at chromosome I

    DEFF Research Database (Denmark)

    Sørensen, Kirsten Kørup; Kirk, Hanne Grethe; Olsson, Kerstin;

    2008-01-01

    New potato (Solanum tuberosum) varieties are required to contain low levels of the toxic glycoalkaloids and a potential approach to obtain this is through marker-assisted selection (MAS). Before applying MAS it is necessary to map quantitative trait loci (QTLs) for glycoalkaloid content in potato...... and the HAF parent. Quantitative trait loci for glycoalkaloid production in foliage of different Solanum species have previously been mapped to this chromosome. In the present research, QTLs for α-solanine and α-chaconine content were mapped to the same location as for TGA content. Similar results...

  7. Evaluación de los procesos de precocción/congelación de tres presentaciones de papa criolla (Solanum tuberosum grupo phureja) variedad Colombia / Assessment processes precooking/freezing of three presentations of native potato (Solanum tuberosum phureja group) Colombia variety

    OpenAIRE

    Mendoza Rincón, Rolando

    2012-01-01

    El presente trabajo tiene como objetivo estandarizar los procesos de precocción y congelación de tres presentaciones de papa criolla (Solanum tuberosum grupo phureja). Se usaron presentaciones de tubérculos enteros de 2,7 cm de diámetro, semiesferas a partir de tubérculos de 3,5 cm de diámetro y bastones (prismas rectangulares de 0,7 cm x 0,8 cm x 2,8 cm). Para estandarizar la etapa de precocción se generaron modelos matemáticos de transferencia de calor acoplados a modelos de inactivación de...

  8. Cinética de Inactivación de la Enzima Peroxidasa, Color y Textura en Papa Criolla (Solanum tuberosum Grupo phureja) sometida a tres Condiciones de Escaldado Kinetics of Peroxidase Enzyme Inactivation, Color and Texture in Golden Potato (Solanum tuberosum phureja group) under three Blanching Conditions

    OpenAIRE

    Rolando Mendoza; Aníbal O Herrera

    2012-01-01

    El objetivo de este trabajo fue determinar la cinética de inactivación de la enzima peroxidasa y la cinética del color y textura en tubérculos de papa criolla (Solanum tuberosum grupo phureja) sometidos a escaldado mediante agua a 80°C, 90°C y vapor saturado a 93°C. Los coeficientes de transferencia de calor para el escaldado en agua oscilaron entre 214 y 230 W °C-1 m-2 mientras que para vapor saturado fueron en promedio 84.5 W °C-1 m-2. A diferentes tiempos de precocción el tubérculo de papa...

  9. Ocorrência de pectobactérias em tubérculos de batata-semente no estado do Rio Grande do Sul Ocurrence of pectobacteria in potato seed tubers in Rio Grande do Sul

    OpenAIRE

    Samira O. M. El Tassa; Valmir Duarte

    2004-01-01

    Tubérculos de batata (Solanum tuberosum)-semente, pré-básica, básica, registrada e certificada, de oito cultivares, oriundos de 21 lavouras localizadas nos municípios de Vacaria, Canguçu, Piratini e Ibiraiaras, no Rio Grande do Sul, foram coletados nos meses de maio a agosto de 2002. Cada tubérculo foi lavado em água corrente, deixado secar à temperatura ambiente, perfurado com palitos em dez lenticelas, coberto com fina camada de óleo de soja, colocado individualmente em cima de folha de pap...

  10. Influence of the time of application of 32P triple Superphosphate on the efficiency of the phosphate fertilization and the productivity of the potato (Solanum Tuberosum L.)

    International Nuclear Information System (INIS)

    A study was performed on a soil derived from volcanic ash (Andic Humitropept), in Pasto (Narino), Colombia, to investigate the influence of additions of 100 and 200 Kg P2O5ha-1, at two application times (planting and 30 days after emergence - DAE), on the fertilizer use efficiency and productivity of potato (Solanum tuberosum L.) cv. Pardo Pastusa. At planting the phosphorus was applied in a continuous row in the furrow, and at 30 DAE at one side of the plant row, and immediately covered with soil from both sides of the furrow. The fertilizer used was 32P labelled triple superphosphate (143.59 μ Ci.g-1p-1. It was found that: a) the phosphorus fertilization significantly increased (P-1) and the accumulation (from 8 to 17.8 kg P ha-1) by the tubers, but there was no significant effect of the different rates or times of the fertilizer application; b) the accumulation of P by the plant accompanied the accumulation of plant dry matter; c) at 75 DAE the fraction of fertiliser P in the plant shoot in the treatment fertilized at 30 DAE (52.1%) was significantly higher than that fertilized at planting (21.8%), and this corresponded to a P fertilizer use efficiency of 2.4 and 0.82, respectively

  11. PG-2, a Potent AMP against Pathogenic Microbial Strains, from Potato (Solanum tuberosum L cv. Gogu Valley Tubers Not Cytotoxic against Human Cells

    Directory of Open Access Journals (Sweden)

    Yoonkyung Park

    2013-02-01

    Full Text Available In an earlier study, we isolated potamin-1 (PT-1, a 5.6-kDa trypsin-chymotrypsin protease inhibitor, from the tubers of a potato strain (Solanum tuberosum L cv. Gogu Valley. We established that PT-1 strongly inhibits pathogenic microbial strains, but not human bacterial strains, and that its sequence shows 62% homology with a serine protease inhibitor. In the present study, we isolated an antifungal and antibacterial peptide with no cytotoxicity from tubers of the same potato strain. The peptide (peptide-G2, PG-2 was isolated using salt-extraction, ultrafiltration and reverse-phase high performance liquid chromatography (RP-HPLC. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS showed the protein to have a molecular mass of 3228.5 Da, while automated Edman degradation showed the N-terminal sequence of PG-2 to be LVKDNPLDISPKQVQALCTDLVIRCMCCC-. PG-2 exhibited antimicrobial activity against Candida albicans, a human pathogenic yeast strain, and Clavibacter michiganensis subsp. michiganensis, a plant late blight strain. PG-2 also showed antibacterial activity against Staphylococcus aureus, but did not lyse human red blood cells and was thermostable. Overall, these results suggest PG-2 may be a good candidate to serve as a natural antimicrobial agent, agricultural pesticide and/or food additive.

  12. Purification of a novel chitin-binding lectin with antimicrobial and antibiofilm activities from a bangladeshi cultivar of potato (Solanum tuberosum).

    Science.gov (United States)

    Hasan, Imtiaj; Ozeki, Yasuhiro; Kabir, Syed Rashel

    2014-04-01

    A new chitin-binding lectin was purified from a Bangladeshi cultivar 'Deshi' of potato (Solanum tuberosum L.) through anion-exchange and affinity chromatographies using a chitin column. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed the molecular mass of the lectin as 20,000 Daltons. This molecular mass was almost half of the molecular masses of chitin-binding lectins derived from other potatoes. The lectin showed both bactericidal and growth-inhibiting activities against Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli, Salmonella enteritidis and Shigella boydii) pathogenic bacteria. It also showed antifungal activity against Rhizopus spp., Penicillium spp. and Aspergillus niger. Biofilm produced by the bacterium Pseudomonas aeruginosa was dose-dependently reduced by 5-20% in 24 h after administration of the lectin, which was attributed to the glycan-binding property of the lectin having affinity to GlcNAc polymers. It was the first observation that any potato lectin prevented biofilm formation by P. aeruginosa and, therefore, could have possible applications in clinical microbiology and biomedical science. PMID:24980018

  13. The effect of in vitro mycorrhization on growth characteristics, changes in endogenous hormones and performance of microplants in potato (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Khosro PARVIZI

    2015-12-01

    Full Text Available Potato (Solanum tuberosum L. plantlets were inoculated in vitro with an arbuscular mycorrhizal fungus (Glomus intraradices and their growth response, performance and endogenous hormonal status evaluated. A factorial experiment based on a completely randomized design consisting of two potato cultivars (Agria and Sante and four culture media was conducted with four replications. The culture media included non-inoculated Murashige and Skoog (MS medium (control, inoculated MS medium, inoculated half-strength MS medium and inoculated MS medium without vitamins and sugar. To do inoculation, germinated spores of fungus were transferred to the root zone of plantlets. Colonization percentage, total chlorophyll content, internodes and stolon length, shoot diameter, shoot and root fresh and dry weight, leaf area and the level of three endogenous hormones (total auxins, gibberellins and cytokinins were determined. In addition, mini-tuber production was assayed quantitatively and qualitatively. Results demonstrated that the effect of two factors on all characteristics including mini-tuber production was different significantly. Interaction between cultivar and inoculation systems was significantly associated with endogen auxins as well as all range of mini-tuber production. The inoculated MS medium showed the better results, but it did not have significant difference to half-strength MS in terms of plantlet performance and growing parameters. Responses of both cultivars to inoculation were very conspicuous in the production of endogen hormones. Higher endogen hormone levels were associated with elevated growth parameters, greater biomass production and better plantlet performance.

  14. Differences in potato development (Solanum tuberosum cv. Maris Piper) in zero and conventional traffic treatments are related to soil physical conditions and radiation interception

    International Nuclear Information System (INIS)

    Penetration resistance, potato (Solanum tuberosum cv. Maris Piper) root, tuber, and top growth, and leaf nutrient concentrations were compared between a conventional traffic (CTr) and a zero traffic (ZTr) treatment. Plants grown under ZTr had a 19.4% greater yield (P < 0.001), and throughout the growing season leaf area, stem dry weight, stolon and tuber dry weight, were consistently greater for ZTr plants. On three occasions during the growing season penetration resistance was greater in CTr furrow subsoil than in ZTr furrow subsoil, as a result of the drier conditions in CTr soil. ZTr soil in the ridge had greater air-filled porosities throughout the growing season, although between mid-May and mid-July the furrow subsoil of both ZTr and CTr were at or near saturation. Although no significant differences in root distribution of plants were observed between treatments, all roots growing within the subsoil were elongating in cracks and biopores. Determination of radiation interception values from leaf area and radiation data, showed that between 5 July and 20 August ZTr plants were intercepting 5% more radiation (P < 0.01) than CTr plants. No differences in light conversion coefficients were observed for plants in either traffic treatment. The observed yield differences are linked primarily to the differences in radiation interception between the two treatments

  15. Reduction of the plastidial phosphorylase in potato (Solanum tuberosum L.) reveals impact on storage starch structure during growth at low temperature.

    Science.gov (United States)

    Orawetz, Tom; Malinova, Irina; Orzechowski, Slawomir; Fettke, Joerg

    2016-03-01

    Tubers of potato (Solanum tuberosum L.), one of the most important crops, are a prominent example for an efficient production of storage starch. Nevertheless, the synthesis of this storage starch is not completely understood. The plastidial phosphorylase (Pho1; EC 2.4.1.1) catalyzes the reversible transfer of glucosyl residues from glucose-1-phosphate to the non-reducing end of α-glucans with the release of orthophosphate. Thus, the enzyme is in principle able to act during starch synthesis. However, so far under normal growth conditions no alterations in tuber starch metabolism were observed. Based on analyses of other species and also from in vitro experiments with potato tuber slices it was supposed, that Pho1 has a stronger impact on starch metabolism, when plants grow under low temperature conditions. Therefore, we analyzed the starch content, granule size, as well as the internal structure of starch granules isolated from potato plants grown under low temperatures. Besides wild type, transgenic potato plants with a strong reduction in the Pho1 activity were analyzed. No significant alterations in starch content and granule size were detected. In contrast, when plants were cultivated at low temperatures the chain length distributions of the starch granules were altered. Thus, the granules contained more short glucan chains. That was not observed in the transgenic plants, revealing that Pho1 in wild type is involved in the formation of the short glucan chains, at least at low temperatures. PMID:26828405

  16. Are uranium-contaminated soil and irrigation water a risk for human vegetables consumers? A study case with Solanum tuberosum L., Phaseolus vulgaris L. and Lactuca sativa L.

    Science.gov (United States)

    Neves, O; Abreu, M M

    2009-11-01

    The knowledge of uranium concentration, in the products entering the human diet is of extreme importance because of their chemical hazard to health. Controlled field experiments with potatoes, beans and lettuce (Solanum tuberosum L., Phaseolus vulgaris L. and Lactuca sativa L.) were carried out in a contaminated soil used by local farmers located near a closed Portuguese uranium mine (Cunha Baixa, Mangualde). The soil with high average uranium levels (64-252 mg/kg) was divided in two plots, and irrigated with non-contaminated and uranium-contaminated water (900 microg/L). Uranium maximum average concentration in the edible vegetables parts (mg/kg fresh weight) ranged in the following order: lettuce (234 microg/kg) > green bean (30 microg/kg) > potatoes without peel (4 microg/kg). Although uranium in soil, irrigation water and vegetables was high, the assessment of the health risk based on hazard quotient indicates that consumption of these vegetables does not represent potential adverse (no carcinogenic) effects for a local inhabitant during lifetime. PMID:19590953

  17. A recombination point is conserved in the mitochondrial genome of higher plant species and located downstream from the cox2 pseudogene in Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Susely F.S. Tada

    2006-01-01

    Full Text Available The potato (Solanum tuberosum L. mitochondrial cox3/sdh4/pseudo-cox2 gene cluster has previously been identified by heterologous hybridization using a Marchantia polymorpha sdh4 probe. In our present study we used Southern blotting using sdh4 and cox2 probes to show that the sdh4 and cox2 genes are clustered in the mitochondria of potato, soybean and pea. Northern blotting revealed cotranscription of sdh4 and cox2 in potato but not in cauliflower, indicating that these genes are not clustered in cauliflower. A putative recombination point was detected downstream of the cox2 pseudogene (pseudo-cox2 in potato mitochondrial DNA (mtDNA. This sequence corresponds to a 32 bp sequence which appears to be well-conserved and is adjacent to the terminals of some mitochondrial genes in Citrullus lanatus, Beta vulgaris and Arabidopsis thaliana and is probably involved in the genic rearrangements. It is possible the potato mtDNA pseudo-cox2 gene was generated by recombination during evolution in the same way as that of several other mitochondrial genes and remains as an inactive partial copy of the functional cox2 which was also detected in potato mtDNA.

  18. Estudio de libertad de operación para una línea genéticamente modificada de papa (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Alejandro Chaparro-Giraldo

    2014-06-01

    Full Text Available Título en ingles: Study of freedom to operate for a genetically modified potato (Solanum tuberosum L. lineTítulo corto: Libertad de operación para papa GMResumen: Durante el desarrollo de los productos biotecnológicos son utilizados materiales y procesos, que pueden estar protegidos por derechos de propiedad intelectual. Para evitar problemas legales en su comercialización, se deben realizar estudios de libertad de operación. Este estudio se realizó sobre una línea genéticamente modificada (GM de papa (Solanum tuberosum L. derivada de la variedad Pastusa Suprema, que expresa el gene Cry1Ac de Bacillus thuringiensis, desarrollada por la Corporación de Ciencias Biológicas y la Universidad Nacional de Colombia, Sede Medellín. El punto de partida, fue la deconstrucción del producto, cuyo resultado fue la lista de materiales y procesos usados en el desarrollo del producto. Se buscaron en bases de datos nacionales e internacionales de acceso público, las solicitudes de patentes y patentes relacionadas. En el nivel internacional, se encontraron cuatro solicitudes de patentes y dieciocho patentes relacionadas, la mayoría de las cuales, no han sido solicitadas en Colombia. En el nivel nacional, se encontraron 13 solicitudes de patentes, que han caducado, han sido negadas, abandonadas, desistidas, o están en requerimiento. Se encontró que la variedad tiene registro comercial, pero no título de obtentor. También se examinaron documentos de las instituciones participantes, que contuvieran cláusulas sobre propiedad intelectual, y otros documentos de interés, como los acuerdos de transferencia de materiales (ATM. Se concluye que la libertad de operación puede estar afectada más por problemas detectados en los ATM y en la complejidad de los acuerdos interinstitucionales suscritos, que por los derechos de propiedad intelectual.Palabras clave: Derechos de propiedad intelectual, biotecnología, cultivos transgénicos.Abstract:  During the

  19. Detección Serológica y Molecular del Virus PVYN y su variante PVYNTN en Papa (Solanum tuberosum L.) y Hospedantes Alternos en Tapalpa, México

    OpenAIRE

    Miguel Hernández-de la Cruz; Juan Florencio Gómez-Leyva; Irma Guadalupe López-Muraira; María Susana Dimas-Estrada; Isaac Andrade-González; Javier Ireta-Moreno

    2007-01-01

    Se realizó un muestreo en cultivos de papa (Solanum tuberosum) para semilla en la zona productora de Tapalpa, Jalisco, México, así como de maleza e insectos asociados al cultivo con el objetivo de determinar la presencia del virus PVYN y su variante PVYNTN. Mediante técnicas serológicas y moleculares se analizaron 41 variedades de papa, 19 de Tapalpa, y 22 provenientes de Toluca, Estado de México. Con la técnica de DAS-ELISA, la variedad Malinche de Tapalpa fue positiva a PVYN, mientras que M...

  20. COMPORTAMIENTO DE LA ACUMULACIÓN Y DISTRIBUCIÓN DE MASA SECA EN TRES VARIEDADES DE PAPA (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Eduardo Jerez Mompié

    2015-01-01

    Full Text Available El trabajo se desarrolló en áreas experimentales del Instituto Nacional de Ciencias Agrícolas (INCA durante plantaciones realizadas en el mes de enero de los años 2010, 2011 y 2012, con el objetivo de evaluar el comportamiento de la acumulación y distribución de masa seca entre los diferentes órganos de la planta. Se utilizaron tubérculos semillas importados de tres variedades de papa ( Solanum tuberosum L. Call White, Santana y Spunta, plantadas en tres parcelas (una por cada variedad siguiendo un diseño muestral. A los 40 y 70 días después de la plantación y en la cosecha, se muestrearon 10 plantas al azar por cada variedad, para conocer mediante secado en estufa la cantidad de masa seca en los diferentes órganos. Durante el ciclo del cultivo se registraron las temperaturas medias, máximas y mínimas ocurridas en ese periodo. Se detectaron diferencias intervarietales en la magnitud de los valores alcanzados en la acumulación de masa seca, aun cuando las tres siguen un mismo patrón de crecimiento. Se destaca que al inicio de la plantación la demanda por masa seca es mucho mayor en el follaje, lo cual se invierte hacia los tubérculos al final del mismo. Se discute la influencia de las temperaturas en el crecimiento en general, lo cual se reflejó en la producción de masa seca.

  1. In vitro development and improvement of chromium (vi)-affected adventitious roots of solanum tuberosum l. with ga/sub 3/ and iaa application

    International Nuclear Information System (INIS)

    This study was undertaken In vitro to investigate the inhibitory effects of Chromium (Cr) VI by using K/sub 2/Cr/sub 2/O/sub 7/ on the growth of adventitious roots of potato (Solanum tuberosum L. cv Desiree). Another objective was to determine possible reversal of the detrimental growth effects with suitable combinations of GA/sub 3/ and IAA. It was observed that the applied Cr VI (100 ppm) alone in MS media inhibited root growth. However, addition of selected combinations of GA/sub 3/ and IAA in the MS media along with Cr VI improved the root growth. The plants grown on MS medium + 100 ppm K/sub 2/Cr/sub 2/O/sub 7/ resulted in reduction in number of adventitious roots up to 36.26%, number of rootlets (80.64%), diameter (37.27%), fresh and dry weights (72.96 and 84.74%, respectively) in comparison with control (MS medium). MS media containing 100 ppm K/sub 2/Cr/sub 2/O/sub 7/ + 20 ppm GA/sub 3/ + 16 ppm IAA enhanced the average number of roots from 4.78 to 62.6, number of rootlets from 2.6 to 28.2, diameter from 0.13 mm to 0.68 mm, fresh weight from 34.4 mg to 1234.75 mg and dry weight from 1.8 mg to 80.40 mg compared with cultures grown on MS + 100 ppm Cr (VI). The results thus highlight the fact that the deleterious effects of Cr VI on potato growth In vitro can be reversed with suitable hormonal treatments. Furthermore, this research has possible implications on large scale potato cultivation under heavy metal stress in a broader sense. (author)

  2. Evaluación financiera del sistema de producción de papa (Solanum tuberosum L en Oicatá, Boyacá

    Directory of Open Access Journals (Sweden)

    Elmer Avendaño-Gómez

    2015-07-01

    Full Text Available La factibilidad financiera es fundamental para tomar decisiones en un sistema productivo; en consecuencia, toda actividad agropecuaria debe hacer uso de dicha herramienta, a fin de obtener mayor comprensión de su ejercicio financiero y, a la vez, tener un soporte objetivo para determinar, entre otras cosas, si continuar con la actividad, diversificarla, combinarla o arrendar el espacio físico donde se realiza (1. El objetivo de este trabajo fue realizar una evaluación financiera en el sistema de producción de la papa Solanum tuberosum, variedad Diacol capiro, de manera participativa y con la inclusión de indicadores históricos, en función de horizontes de tiempo y tasa de oportunidad. La población objeto del estudio correspondió a 32 agricultores de papa inscritos en la Empresa Prestadora de Servicios Técnicos Agropecuarios (Epsagro del municipio de Oicatá, departamento de Boyacá, para el año 2014, de los cuales se seleccionaron tres productores (grande, mediano y pequeño para realizar la investigación. Los resultados indican que el diagnóstico rural participativo fue una herramienta de investigación adecuada para la compilación de la información, además de la existencia de diferencias en la estructura de costos de producción en los diferentes agricultores. En el municipio de Oicatá se encontró que existen fallas relevantes a nivel operativo y contable que deben ser corregidas.

  3. EMPLEO DE DOS ANÁLOGOS DE BRASINOESTEROIDES EN LA FORMACIÓN DE CALLOS EMBRIOGÉNICOS EN PAPA (Solanum tuberosum L

    Directory of Open Access Journals (Sweden)

    Olivia Moré

    2001-01-01

    Full Text Available Se evaluó la actividad biológica de los análogos de brasinoesteroides MH5 y Biobras-6 (BB-6, con el objetivo de determinar sus efectos y establecer dosis adecuadas como posibles sustitutos o complementos hormonales en el proce- so de formación de callos embriogénicos en papa (Solanum tuberosum, L. var. Desirée. Se utilizaron dosis de 0.0001 hasta 0.25 mg.L-1 de ambos análogos y dos medios controles, los cuales contenían: 3 mg.L-1 de 2,4D y 0.84 mg.L-1 de kinetina (medio I y 0.5 mg.L-1 de 2,4D y 2 mg.L-1 de kinetina (medio II. Se realizaron evaluaciones atendiendo a los cambios cualita- tivos y cuantitativos de la evolución del callo y con las mejo- res variantes se elaboraron las curvas de crecimiento callogénico. Los resultados indicaron una actividad tipo citoquinina de estos compuestos y un favorecimiento en la obtención de un callo embriogénico de mejor calidad a las concentraciones de 0.1 mg.L-1 de MH5 y 0.01 mg.L-1 de Biobras-6 para el medio I y 0.001 mg.L-1 de MH5 y 0.01 mg.L-1 de Biobras-6 para el medio II. No se encontró respuesta callogénica al ser utilizados estos análogos como sustitutos del 2,4D. Se pudo apreciar un mejor comportamiento en la dinámica de crecimien- to de los callos, en aquellos tratamientos donde se emplearon los productos como sustitutos de la kinetina para los medios I y II.

  4. Curdlan β-1,3-glucooligosaccharides induce the defense responses against Phytophthora infestans infection of potato (Solanum tuberosum L. cv. McCain G1) leaf cells.

    Science.gov (United States)

    Li, Jing; Zhu, Li; Lu, Guangxing; Zhan, Xiao-Bei; Lin, Chi-Chung; Zheng, Zhi-Yong

    2014-01-01

    Activation of the innate immune system before the invasion of pathogens is a promising way to improve the resistance of plant against infection while reducing the use of agricultural chemicals. Although several elicitors were used to induce the resistance of potato plant to microbial pathogen infection, the role of curdlan oligosaccharide (CurdO) has not been established. In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv. McCain G1). The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves. In addition, glucopentaose was proved to be the shortest active curdlan molecule based on the accumulation of H₂O₂ and salicylic acid and the activities of phenylalanine amino-lyase, β-1,3-glucanase and chitinase. The 2D-PAGE analysis reveals that CurdOs activate the integrated response reactions in potato cells, as a number of proteins with various functions are up-regulated including disease/defense, metabolism, transcription, and cell structure. The pathogenesis assay shows that the ratio of lesion area of potato leaf decreased from 15.82%±5.44% to 7.79%±3.03% when the plants were treated with CurdOs 1 day before the infection of Phytophthora infestans. Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually. PMID:24816730

  5. Curdlan β-1,3-glucooligosaccharides induce the defense responses against Phytophthora infestans infection of potato (Solanum tuberosum L. cv. McCain G1 leaf cells.

    Directory of Open Access Journals (Sweden)

    Jing Li

    Full Text Available Activation of the innate immune system before the invasion of pathogens is a promising way to improve the resistance of plant against infection while reducing the use of agricultural chemicals. Although several elicitors were used to induce the resistance of potato plant to microbial pathogen infection, the role of curdlan oligosaccharide (CurdO has not been established. In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv. McCain G1. The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves. In addition, glucopentaose was proved to be the shortest active curdlan molecule based on the accumulation of H₂O₂ and salicylic acid and the activities of phenylalanine amino-lyase, β-1,3-glucanase and chitinase. The 2D-PAGE analysis reveals that CurdOs activate the integrated response reactions in potato cells, as a number of proteins with various functions are up-regulated including disease/defense, metabolism, transcription, and cell structure. The pathogenesis assay shows that the ratio of lesion area of potato leaf decreased from 15.82%±5.44% to 7.79%±3.03% when the plants were treated with CurdOs 1 day before the infection of Phytophthora infestans. Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually.

  6. Effects of elevated carbon dioxide and ozone on the growth and yield of potatoes (Solanum tuberosum) grown in open-top chambers

    International Nuclear Information System (INIS)

    Potato (Solanum tuberosum cv. Bintje) was grown in open-top chambers under three carbon dioxide (ambient and seasonal mean concentrations of 550 and 680 μmol mol-1 CO2) and two ozone concentrations (ambient and an 8 h day -1 seasonal mean of 50 nmol mol-1 O3) between emergence and final harvest. Periodic non-destructive measurements were made and destructive harvests were carried out at three key developmental stages (24, 49 and 101 days after emergence) to establish effects on growth and tuber yield. Season-long exposure to elevated O3 reduced above-ground dry weight at final harvest by 8.4% (P2 and O3 for any of the growth and yield variables examined. Non-destructive analyses revealed no significant effect of elevated CO2 on plant height, leaf number or green leaf area ratio. However, destructive harvests at tuber initiation and 500 deg Cd after emergence showed that above-ground dry weight (8 and 7% respectively) and tuber yield (88 and 44%) were significantly increased (P-1 CO2 treatment. Responses to 550 and 680 μmol mol-1 CO2 were not significantly different for most parameters examined, suggesting the existence of an upper limit to the beneficial influence of CO2 enrichment. Significant effects on the above-ground dry weight and tuber yield were no longer apparent at final harvest, although tuber numbers were increased (P2, particularly in the smaller size categories. The results show that the O3 treatment imposed was insufficient to reduce tuber yields and that, although elevated CO2 enhanced crop growth during the early stages of the season, this beneficial effect was not sustained to maturity. (Author)

  7. Isolation and characterization of a β-propeller gene containing phosphobacterium Bacillus subtilis strain KPS-11 for growth promotion of potato (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Kashif eHanif

    2015-06-01

    Full Text Available Phosphate-solubilizing and phytate-mineralizing bacteria collectively termed as phosphobacteria provide a sustainable approach for managing P-deficiency in agricultural soils by supplying inexpensive phosphate to plants. A phosphobacterium Bacillus subtilis strain KPS-11 (Genbank accession no. KP006655 was isolated from potato (Solanum tuberosum L. rhizosphere and characterized for potato plant growth promoting potential. The strain utilized both Ca-phosphate and Na-phytate in vitro and produced 6.48 µg mL-1 indole-3-acetic acid in tryptophan supplemented medium. P-solubilization after 240 h was 66.4 µg mL-1 alongwith the production of 19.3 µg mL-1 gluconic acid and 5.3 µg mL-1 malic acid. The extracellular phytase activity was higher (4.3 x 10-10 kat mg-1 protein than the cell-associated phytase activity (1.6 x 10-10 kat mg-1protein. B. subtilis strain KPS-11 utilized 40 carbon sources and showed resistance against 20 chemicals in GENIII micro-plate system demonstrating its metabolic potential. Phytase-encoding gene β-propeller (BPP showed 92% amino acid similarity to BPP from B. subtilis (accession no.WP_014114128.1 and 83% structural similarity to BPP from B. subtilis (accession no 3AMR_A. Potato inoculation with B. subtilis strain KPS-11 increased the root/shoot length and root/shoot weight of potato as compared to non-inoculated control plants. Moreover, rifampicin-resistant derivative of KPS-11 were able to survive in the rhizosphere and on the roots of potato up to sixty days showing its colonization potential. The study indicates that B. subtilis strain KPS-11 can be a potential candidate for development of potato inoculum in P-deficient soils.

  8. Cinética de Inactivación de la Enzima Peroxidasa, Color y Textura en Papa Criolla (Solanum tuberosum Grupo phureja sometida a tres Condiciones de Escaldado Kinetics of Peroxidase Enzyme Inactivation, Color and Texture in Golden Potato (Solanum tuberosum phureja group under three Blanching Conditions

    Directory of Open Access Journals (Sweden)

    Rolando Mendoza

    2012-01-01

    Full Text Available El objetivo de este trabajo fue determinar la cinética de inactivación de la enzima peroxidasa y la cinética del color y textura en tubérculos de papa criolla (Solanum tuberosum grupo phureja sometidos a escaldado mediante agua a 80°C, 90°C y vapor saturado a 93°C. Los coeficientes de transferencia de calor para el escaldado en agua oscilaron entre 214 y 230 W °C-1 m-2 mientras que para vapor saturado fueron en promedio 84.5 W °C-1 m-2. A diferentes tiempos de precocción el tubérculo de papa criolla presentó una zona en donde ocurrió pardeamiento enzimático, hecho que fue modelado según la cinética de Michaelis-Menten y otra zona en donde el cambio de color disminuyó debido a la inactivación enzimática que correspondió a una cinética de primer orden, de manera similar al comportamiento de la textura. La inactivación de la enzima peroxidasa obedeció al mecanismo de Lumry-Eyring.The objective of this work was to determine the inactivation kinetics of peroxidase enzyme and the change in color and texture in golden potato tubers (Solanum tuberosum phureja group exposed to blanching with water at 80°C, 90°C and to saturated steam at 93°C. The heat transfer coefficients for the blanch water ranged between 214 and 230 W °C-1 m-2 while for saturated steam they showed an average of 84.5 W °C-1 m-2. At different pre-cooking times the golden potato tuber showed an area that developed enzymatic browning, which was fitted to the Michaelis-Menten kinetic model and another area where the color change decreased due to enzyme inactivation, which corresponded to first order kinetics, in a similar way as the texture behavior. The inactivation of peroxidase enzyme followed the Lumry-Eyring mechanism.

  9. Simulação do desenvolvimento da batata cultivar asterix em cinco cenários de mudanças climáticas em Santa Maria, RS Simulation of potato cultivar asterix development under five climate change scenarios in Santa Maria

    OpenAIRE

    Nereu Augusto Streck; Isabel Lago; Cleber Maus Alberto; Dilson Antônio Bisognin

    2006-01-01

    Em estudos do impacto de mudanças climáticas sobre agroecossistemas, um aspecto importante a quantificar é o efeito do aquecimento global sobre o desenvolvimento vegetal. O objetivo desse trabalho foi simular o impacto de cenários climáticos de aumento de temperatura do ar no desenvolvimento da batata (Solanum tuberosum L.), em Santa Maria, (RS). Cenários meteorológicos de mudanças climáticas (+2 °C, +3 °C, +4 °C, +5 °C e +6 °C) para os próximos cem anos foram gerados com ...

  10. Reação de cultivares de batata à podridão mole causada por Pectobacterium carotovorum subsp. atrosepticum, por P. carotovorum subsp. carotovorum e por P. chrysanthemi Reaction of potato cultivars to soft rot caused by Pectobacterium carotovorum subsp. atrosepticum, P. carotovorum subsp. carotovorum and P. chrysanthemi

    OpenAIRE

    Adilce I. H. Benelli; Norimar D. Denardin; CARLOS A. FORCELINI; Valmir Duarte

    2004-01-01

    A podridão mole em tubérculos de batata (Solanum tuberosum), causada por Pectobacterium carotovorum subsp. atrosepticum, por Pectobacterium carotovorum subsp. carotovorum e por P. chrysanthemi, é uma preocupante doença que causa danos expressivos à cultura em todo o mundo. Como inexiste tratamento eficiente para a podridão mole, o desenvolvimento de cultivares resistentes é considerado o método mais eficaz para a redução de perdas causadas pela doença. Nesse sentido, quatro cultivares de bata...

  11. Agro-transformation and evaluation of resistance to Phytophthora infestansin Solanum tuberosumL. variety Désirée

    Directory of Open Access Journals (Sweden)

    Jeanette Orbegozo

    2014-03-01

    Full Text Available The Oomycete Phytophthora infestans (Mont. de Bary, the causal agent of the disease known as late blight, is primarily responsible for the decreased in production performance and potato crops worldwide. The integration of the complete Rgenes sequences in the potato genome using Agro-transformation appears an alternative to be considered in the fight against this pathogen. The Rpi-blb2 gene (Rgene from the wild species Solanum bulbocastanumDunal shows a broad resistance to isolates ofP. infestans,making it an important candidate for plant breeding studies. This paper reports the integration of the Rpi-blb2gene into potato var. Désirée genome by Agrobacterium tumefaciens- mediated transformation system, the molecular characterization of 29 events transformed and whole plant infection with isolate POX67 of P. infestansfrom Peru. Désirée events [Rpi-blb2] 4 and Désirée [Rpi-blb2] 30, showed a substantial resistance to P. infestansinfection confirming complete transfer of the Rpi-blb2gene from a wild species to a cultivated species by genetic transformation.

  12. Identification of Differentially Expressed Genes in Chilling-Induced Potato (Solanum tuberosum L.); a Data Analysis Study.

    Science.gov (United States)

    Koc, I; Vatansever, R; Ozyigit, I I; Filiz, E

    2015-10-01

    Cold stress, as chilling (potato. Under cold stress, plants differentially modulate their gene expression to develop a cold tolerance/acclimation. In the present study, we aimed to identify the overall gene expression profile of chilling-stressed (+4 °C) potato at four time points (4, 8, 12, and 48 h), with a particular emphasis on the genes related with transcription factors (TFs), phytohormones, lipid metabolism, signaling pathway, and photosynthesis. A total of 3504 differentially expressed genes (DEGs) were identified at four time points of chilling-induced potato, of which 1397 were found to be up-regulated while 2107 were down-regulated. Heatmap showed that genes were mainly up-regulated at 4-, 8-, and 12-h time points; however, at 48-h time point, they inclined to down-regulate. Seventy five up-regulated TF genes were identified from 37 different families/groups, including mainly from bHLH, WRKY, CCAAT-binding, HAP3, and bZIP families. Protein kinases and calcium were major signaling molecules in cold-induced signaling pathway. A collaborated regulation of phytohormones was observed in chilling-stressed potato. Lipid metabolisms were regulated in a way, highly probably, to change membrane composition to avoid cold damage and render in signaling. A down-regulated gene expression profile was observed in photosynthesis pathway, probably resulting from chilling-induced reduced enzyme activity or light-triggered ROSs damage. The findings of this study will be a valuable theoretical knowledge in terms of understanding the chilling-induced tolerance mechanisms in cultivated potato plants as well as in other Solanum species. PMID:26260485

  13. Resistência de genótipos de batata (Solanum spp. a Diabrotica speciosa (Germar, 1824 (Coleoptera: Chrysomelidae Resistence of potatoes genotypes (Solanum spp. to Diabrotica speciosa (Germar,1824 (Coleoptera: Chrysomelidae

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    Fernando Mesquita Lara

    2000-12-01

    Full Text Available Avaliou-se a resistência de genótipos de batata (Solanum spp. a Diabrotica speciosa (Germar,1824 em condições de campo e laboratório. O plantio de campo foi efetuado em julho de 1997, na FCAV/ UNESP, Campus de Jaboticabal, utilizando-se os seguintes genótipos: Achat, NYL 235-4, 288.759-16, 288. 771-6, 288.776-3, 288.776-6, 288.787-5, 288.801-8 e 288.814-7. Avaliaram-se o consumo de área foliar em levantamentos realizados aos 70 e 90 dias após plantio e os danos ocasionados pelas larvas aos tubérculos, após a colheita. Em laboratório, foram realizados testes de preferência para alimentação com plantas de 40 dias (teste sem chance de escolha, envolvendo os nove genótipos presentes no campo, e com plantas de 47 e 68 dias (com e sem chance de escolha, avaliando-se cinco genótipos. Constatou-se, no ensaio de campo, que os genótipos 288.759-16, 288.776-3, 288.776-6, 288.787-5 e 288.814-7 foram menos consumidos por adultos de D. speciosa que a cultivar Achat, porém, em laboratório, 288.759-16 e 288.776-6 não rev elaram essa característica. O consumo em NYL 235-4 e 288.801-8 foi intermediário, não diferindo dos demais. NYL 235-4 e 288.801-8 foram resistentes às larvas, apresentando, em média, menos que 3 furos por tubérculo. O genótipo 288.776-3 destacou-se como resistente a larvas e adultos de D. speciosa, sendo a resistência a adultos do tipo não-preferência para alimentação (antixenose.The resistance of potato genotypes to Diabroticaspeciosa (Germar,1824 (Coleoptera: Chrysomelidae, was evaluated under field and laboratory conditions. The field trial was set up in the experimental farm of FCAV - UNESP, Jaboticabal, SP, and the following genotypes were compared: Achat, NYL 235-4, 288.759-16, 288.771-6, 288.776-3, 288.776-6, 288.787-5, 288.801-8 e 288.814-7. The leaf area consumption by adults was evaluated at 70 and 90 days after planting, and, after harvest, larval damage to tubers (number of perforations was assessed

  14. Effect of different growth media on number of minitubers and concentration of elements in potato (Solanum tuberosum L shoot and root

    Directory of Open Access Journals (Sweden)

    M. Hajiaghaei Kmrani

    2012-09-01

    Full Text Available Potato (Solanum tuberosum L. is one of the most important economical vegetable. Minitubers are small seed tubers without pathogens that are produced from invitro plantlets after translocation to greenhouse and create healthy and high quality seed tubers. Hydroponic systems increase the production of virus-free potato minitubers. This research was carried out to study the effect of different growth media on number of minitubers and concentration of elements in potato shoot and root and also to compare the hydroponic culture and soil containing media in a randomized complete blocks design with 8 treatments and 4 replications. The treatments consisted of: 1 perlite, 2 perlite + vermiculite (1:1 v/v, 3 perlite + peat moss (1:1 v/v, 4 perlite + soil (1:1 v/v, 5 perlite + soil + compost (40:30:30, 6 perlite + soil + vermicompost (40:30:30, 7 soil, and 8 perlite + peat moss (control. At the end of growth period, the number of tuber per plant and concentration of elements (N, P, K, Na, and Ca in roots and shoots were measured. Results of analysis of variance showed that bulk density, particle density, porosity, EC and pH of different growth media were significantly different (P<0.01. The soil medium had the highest bulk density (1.23 g/cm3, the highest particle density (2.44 g/cm3, the highest EC and pH and the lowest porosity (50%. Therefore, this treatment produced the lowest number of tubers per pot. The maximum concentration of N, P and K in potato shoot and root were in perlite + soil + vermicompost and soil + compost + perlite media. The maximum concentration of Ca in potato shoot was in perlite + soil medium (1.68 mg/g dw. The maximum concentration of Na in potato shoot was in soil + compost + perlite medium (0.35 mg/g dw. Number of minitubers and plantlets’ growth in soilless media (hydroponics were higher than soil media. There were significant correlations between the concentration of elements in root and shoot with the properties of

  15. PRINCIPALES PROBLEMÁTICAS QUE EFECTAN EL DESARROLLO DEL CULTIVO DE LA PAPA (Solanum tuberosum L. EN DIFERENTES MUNICIPIOS DE LA PROVINCIA HUAMBO, ANGOLA

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    Fernando M. de Almeida

    2015-01-01

    Full Text Available En correspondencia con los bajos rendimientos q ue existen en el cultivo de la papa en la provincia de Huambo, Angola, durante el período comprendido desde febrero del 2013 hasta marzo 2014 se desarrolló la presente investigación en cuatro municipios de la provincia, con el objetivo de determinar las principales problemáticas que afectan el desarrollo del cultivo de la papa ( Solanum tuberosum L.. Se realizó un diagnóstico participativo con los productores directos al cultivo, con el uso de diferentes técnicas del desarrollo rural participativo que incluyó entrevistas y cuestionarios como métodos de investigación sociológica. Se realizó un censo de problemas a nivel de agroecosistemas con los productores y sus familias, donde se logró realizar un inventario de todas las dificultades que se encontraban afectando el cultivo de la papa y su sistema de producción actual. Con toda la información básica, se confeccionó una matriz de priorización de problemas y el orden para la solución de los mismos. Se trabajó con una muestra de 212 productores directamente relacionados con la producción de papa, que representan el 57,61 % del total de productores. Los resultados permitieron identificar nueve problemas comunes que afectan el desarrollo de la papa, de ellos los de mayor importancia y frecuencia entre los territorios fueron la mala calidad de las semillas, el uso indistinto de la distancia de plantación y el deficiente manejo agrotécnico de las plantaciones. Los bajos rendimientos del cultivo de la papa en la provincia de Huambo, se encuentran influenciados por irregularidades tecnológicas y costumbres arraigadas no compatibles con las técnicas que normalmente se aplican en el cultivo.

  16. Comparación de dos kits de RT-PCR en la detección de ARNm de dos genes endógenos de papa (Solanum tuberosum spp. Andígena

    Directory of Open Access Journals (Sweden)

    Milet Zabaleta Vanegas

    2009-01-01

    Full Text Available RT-PCR es una técnica en la que usando ARN mensajero como molde, se obtiene complementario o cADN por transcripción inversa, y luego se amplifica uno de los cADN por PCR, mediante el uso de primers específicos. Esta técnica permite realizar estudios de expresión, a nivel de ARN mensajero. Con el propósito de implementar la técnica en papa (Solanum tuberosum spp. Andígena, se utilizaron plántulas cultivadas in vitro de la variedad Pastusa Suprema. Inicialmente se establecieron las condiciones para la extracción de ARN total usando el kit TRizol® Reagent de InvitrogenTM, con el que se obtuvieron excelentes resultados. Este ARN se usó como molde para evaluar dos kits: “ONE Step superScriptTM” y “SuperScriptâ„¢ First Strand Syntesis For RT-PCR SSâ„¢ II RT”, de InvitrogenTM. Se usaron primers específicos para dos genes endógenos: cox y actina. El primero es un gen mi­tocondrial y el segundo es un gen nuclear. Se observaron señales claras y diferenciables de amplificación para cox, utilizando el kit “ONE Step superScriptTM”, con un tamaño esperado de 96 pb. Para actina, se observó una señal clara de amplificación de 300 pb, con el kit “SuperScriptâ„¢ First Strand Syntesis For RT-PCR SSâ„¢ II RT”.  Palabras clave: Solanum tuberosum; cox, actina; RT-PCR; papa; Solanum tuberosum; cox; actin; RT-PCR; potato.

  17. Isolation of cell lines with decreased or deficient nitrate-reductase activity from cell-suspension culture of mono- (2n=x=12) and dihaploid (2n=2x=24) Solanum tuberosum plants

    International Nuclear Information System (INIS)

    In order to obtain in vitro cell lines resistant to high chlorate concentrations, suspension-incubated cells of mono- (2n = x = 12) and dihaploid (2n = 2x = 24) Solanum tuberosum plants were exposed to the action of gamma-rays (500 r/min) doses of 4 and 5 kR. These doses had an inhibiting effect on the plating efficiency and cell survival in the control medium. An increase in the plating efficiency of cells radiated in the selection medium may indicate induced mutagenesis consisting in the loss of nitrate reductase activity in these cells. Ten chlorate-resistant cell lines, selected from radiated cells, successfully developed for 3 years on the selection medium with a high chlorate concentration and when pasaged on the control medium for over a year, have not restored the properties of wild type cells, thus becoming ''stable variants''

  18. Evaluación tecnológica de snacks de papa (Solanum tuberosum L.) obtenidos mediante la aplicación combinada ingeniería de matrices y fritura al vacío

    OpenAIRE

    Hernández Bedoya, Deisy Yulieth

    2014-01-01

    Resumen: Resumen: La presente investigación, tuvo el objetivo de elaborar snacks de papa (Solanum tuberosum L.) variedad Diacol capiro, utilizando la metodología combinada de ingeniería de matrices, mediante la impregnación al vacío (IV) y fritura al vacío. Durante la primera fase del proyecto, se optimizó el proceso de IV, sobre láminas de papa de 3.0± 0.1mm de espesor, adicionando ácido ascórbico (30%VDR/100g), ácido cítrico (100-250) mg/100 g), vitamina E (30%VDR/100g) y NaCl (1% p/p). Com...

  19. Obtención de plantas sanas de papa, Solanum tuberosum L. Variedad salentuna. A través de las técnicas de termoterapia y cultivo de meristemas in vitro.

    Directory of Open Access Journals (Sweden)

    Marulanda Aguirre Adriana

    2001-09-01

    Full Text Available Se obtuvieron plantas Super Elite de papa (Solanum tuberosum L., de la variedad regional Salentuna, catalogada como papa filia, implementando las técnicas de termoterapia, cultivo in vitro de meristemas y multiplicación acelerada. Se limpió esta variedad del complejo de enfermedades que la afectaban, para entregarle a los productores un material sano que los motive a regresar al campo con una alternativa de producción de mayor calidad y rentabilidad. La falta de semilla sana es una necesidad sentida por los agricultores del departamento de Caldas, pues hace más de treinta años que no se renueva el material vegetal y no existen programas de producción de semilla certificad de esta variedad en el país.

  20. Importância de caracteres na dissimilaridade de progênies de batata em gerações iniciais de seleção Importance of characters in the dissimilarity of potato progênies in early generations

    Directory of Open Access Journals (Sweden)

    Giovani Olegário da Silva

    2008-01-01

    Full Text Available O trabalho teve por objetivo verificar as implicações da utilização da análise de importância de caracteres na eliminação de caracteres avaliados para o cálculo da distância genética de progênies de batata (Solanum tuberosum L. nas primeiras gerações de seleção. Os experimentos foram desenvolvidos em casa plástica (geração de seedlings e em campo (primeira geração clonal, na Embrapa Clima Temperado, Pelotas, Estado do Rio Grande do Sul em outono de 2004 e outono de 2005. O estudo demonstrou que a análise de importância de caracteres foi eficiente para diminuir o número de caracteres necessários à classificação das progênies de batata em relação à dissimilaridade. O formato de tubérculo foi o caráter com maior eficiência para o estudo de dissimilaridade em progênies de batata.The objective of the present work was to access the implications of the use of analysis of importance of characters in the elimination of appraised characters for calculating genetic distance of potato (Solanum tuberosum L. progenies in early generations. The experiments were carried out in plastic house (seedling generation and in the field (first clonal generations at Embrapa Temperate Climate, Pelotas, Rio Grande do Sul State in autumn 2004 and 2005. The study was demonstrated the efficiency of the analysis in importance of characters for reducing the number of characters necessary for classifying potato progenies in relation to the dissimilarity. Tuber shape was the character with the largest efficiency for the dissimilarity study in potato progenies.

  1. Solanum tuberosum L. cv Jayoung Epidermis Extract Inhibits Mite Antigen-Induced Atopic Dermatitis in NC/Nga Mice by Regulating the Th1/Th2 Balance and Expression of Filaggrin.

    Science.gov (United States)

    Yang, Gabsik; Cheon, Se-Yun; Chung, Kyung-Sook; Lee, Sung-Jin; Hong, Chul-Hee; Lee, Kyung-Tae; Jang, Dae-Sik; Jeong, Jin-Cheol; Kwon, Oh-Keun; Nam, Jung-Hwan; An, Hyo-Jin

    2015-09-01

    Solanum tuberosum L. cv Jayoung (JY) is a potato with dark purple flesh and contains substantial amounts of polyphenols. In this study, we evaluated the therapeutic effects of S. tuberosum L. cv JY in a mouse model of Dermatophagoides farinae body (Dfb)-induced atopic dermatitis (AD). The ethanol extract of the peel of JY (EPJ) ameliorated Dfb-induced dermatitis severity, serum levels of immunoglobulin E (IgE) and thymus and activation-regulated chemokine. Histological analysis of the skin also revealed that EPJ treatment significantly decreased mast cell infiltration. The suppression of dermatitis by EPJ treatment was accompanied by a decrease in the skin levels of type 2 helper T-cell cytokines such as interleukin (IL)-4, IL-5, and IL-13. The induction of thymic stromal lymphopoietin, which leads to a systemic Th2 response, was also decreased in the skin by EPJ. Nuclear translocation of nuclear factor-κB p65 was decreased by EPJ in Dfb-induced NC/Nga mice. The protein expression of filaggrin in the AD-like skin lesions was restored by EPJ treatment. These results suggested that EPJ may be a potential therapeutic tool for the treatment of AD. PMID:26102094

  2. Variedades de batatinha (Solanum tuberosum L.. I - Comportamento de 12 variedades procedentes da Holanda, Alemanha e Suécia Behavior of twelve potato varieties from European sources

    Directory of Open Access Journals (Sweden)

    O. J. Boock

    1956-01-01

    Full Text Available No presente trabalho são relatadas experiências com 12 variedades de batatinha (Solanum tuberosum L., recebidas da Holanda (Prinslander, Irene, Froma e Barima, Alemanha (Merkur, Sabina, Linda e Concordia e Suécia Konsuragis, Eigenheimer, Voran e Jätte-Bintje. Essas experiências, em número de seis, das quais três no chamado período "da sêca" (março-julho e três no "das águas" (setembro-janeiro, foram executadas nas localidades de Campinas, Louveira o Capão Bonito, no Estado de São Paulo. Ficou evidenciado o seguinte: a dado o bom estado de brotação dos tubérculos, as porcentagens de falhas no plantio "da sêca" foram baixas; já no plantio "das águas", as variedades de brotação lenta, como "Voran" e "Sabina", falharam muito; b "Prinslander" e "Konsuragis" resistiram bem à sêca prolongada, e "Barima" o "Linda" mostraram ser muito sensíveis; c "Linda", "Voran" e "Merkur", principalmente a primeira destas, apresentaram boa resistência a Phytophthora infestans, e "Jätte-Bintje" foi muito suscetível; "Voran", "Irene", "Prinslander" e "Barima" não ofereceram resistência a Alternaria solani; d "Merkur" e "Konsuragis" foram as mais produtivas para as duas épocas de plantio, sendo que "nas águas", "Eigenheimer", "Barima", "Concordia" e Jätte-Bintje", também produziram bem. Outros aspectos relacionados com variedades foram estudados.Twelve potato varieties imported from Holland (Prinslander, Irene, Froma, and Barima, Germany (Merkur, Sabina, Linda, and Concordia, and Sweden (Konsuragis, Eigenheimer, Voran, and Jätte-Bintje were compared in six experiments carried out at three different localities in the state of São Paulo (Campinas, Louveira, and Capão Bonito. The experiments were planted twice a year, once during the so-called dry season (March lo July, and another time during the rainy season (September to March. The following results were obtained: a plantings made in the dry season had a good stand, whereas this was

  3. Detección de Ca Liberibacter solanacearum y fitoplasmas en cultivo de papa (Solanum tuberosum L. en el Valle de Toluca

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    Ana Tarin Gutiérrez-Ibáñez

    2013-06-01

    Full Text Available Título en ingles: Detection of Ca Liberibacter solanacearum and phytoplasma in potato crop (Solanum tuberosum L. in Toluca Valley Título corto: Detección de Ca Liberibacter solanacearum y fitoplasmas Resumen En México y Centro América se han detectado tubérculos de papa con manchado interno. Recientemente en Texas EUA a esta enfermedad se le ha denominado “Zebra Chip” (ZC o rayado de la papa, los síntomas foliares se asemejan al síndrome denominado “Punta Morada de la Papa” (PMP o enfermedad del “amarillamiento por psilidos” la cual es asociada con la presencia de “Candidatus Liberibacter solanacearum”. El objetivo de esta investigación fue detectar la presencia de esta bacteria y de fitoplasmas en plantas de papa que presentaban la coloración purpura de los foliolos. Durante el ciclo primavera – verano 2011 y 2012 se hizo un muestreo en los municipios de Tenango del Valle, Zinacantepec, Villa de Allende y San José del Rincón, del Estado de México. La detección de ambos patógenos se realizó mediante la reacción en cadena de la polimerasa (PCR con los iniciadores específicos para fitoplasmas: P1/P7, R16mF2/R16mR1 y para Ca Liberibacter solanacearum: OA2/Oi2c, resultando el 35,8% de las plantas positivas para fitoplasmas y el 11,6% para la bacteria. Los resultados indican  que en algunas regiones productoras de papa del Estado de México,  los dos presuntos agentes causales del síndrome de  PMP, fitoplasmas y Ca. Liberibacter solanacearum, pueden estar asociados. Palabras clave: Candidatus Liberibacter solanacearum; Fitoplasma; punta morada. Abstract In Mexico and Central America have been detected stained potato tubers with internal browning; recently in Texas, USA, this disease has been called "Zebra Chip" (ZC or striped potato, foliar symptoms resemble the syndrome called "Potato Purple Top" (PPT or "psyllid yellows" disease which is associated with the presence of "Candidatus liberibacter solanacearum

  4. Incidencia de potyvirus y caracterización molecular de PVY en regiones productoras de papa (Solanum tuberosum L. de Colombia

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    José Miguel Cotes

    2011-08-01

    Full Text Available Incidence of potyvirus and molecular characterization of PVY in potato (Solanum tuberosum L. growing regions of ColombiaTítulo corto: Incidencia de potyvirus en cultivos de papa de Colombia ResumenLos problemas virales reducen los rendimientos y la calidad del tubérculo semilla en cultivos de papa de todo el mundo. Esta investigación se planteó con el fin de evaluar los niveles de incidencia de potyvirus en diez de las principales regiones cultivadoras de papa de los departamentos de Antioquia, Boyacá, Cundinamarca y Nariño (Colombia, y las características genotípicas del virus Y de la papa (Potato virus Y, PVY, seleccionado por ser el potyvirus más limitante de este cultivo. Para la evaluación de la incidencia se utilizaron pruebas de Elisa con anticuerpos que reconocen epítopes comunes a los potyvirus, mientras que las pruebas moleculares incluyeron el análisis filogenético de secuencias parciales del gen de la cápside viral de 33 aislamientos, así como la secuenciación de una porción de los extremos 5´ y 3´del genoma de dos cepas colombianas de este virus. Los resultados confirmaron la presencia de potyvirus en los cultivos de los cuatro departamentos evaluados, con una incidencia promedio del 72%, siendo este nivel superior al 56% en todas las zonas evaluadas. Los análisis moleculares del PVY, permitieron asociar las cepas colombianas estudiadas con las razas PVYN y la variante PVYNTN, esta última responsable de la enfermedad conocida en el mundo como PTNRD (Potato tuber necrotic ringspot disease. Palabras clave: cápside, Elisa, RT-PCR, secuenciación.  AbstractPotato viruses are responsible for significant reductions in seed quality and crop yields around the world. In this study, we evaluate the levels of incidence of potyvirus in ten potato growing regions of Colombia from the provinces of Antioquia, Boyacá, Cundinamarca and Nariño. As PVY is the most limiting potyvirus in potato farming, a molecular

  5. Succession of crops in fitossanidade and crop productivity of potato (Solanum tuberosum L.) Sucessão de culturas na fitossanidade e produtividade da cultura da batata (Solanum tuberosum L.) Suceción de culturas na fitosanidad y productividad de la papa (Solanum tuberosum L.)

    OpenAIRE

    Adenilsom dos Santos Lima; Marcio Furlan Maggi; Sidnei Osmar Jadoski; Leimar Brunetta; Rafael Wazne

    2009-01-01

    The study was conducted in the experimental area of Department of Agronomy, campus Cedeteg of the State University Center West, Guarapuava - PR. It aimed to evaluate the productivity and classification of tubers and the severity and incidence of scab on the potato crop in succession crops of vetch (Vicia sativa L.), oat (Avena strigosa L.), wheat (Triticum aestivum L.), radish (Raphanus sativus

  6. Evaluación de dos tipos de esquejes para producción de tubérculo-semilla élite en cuatro variedades de papa Solanum phureja juz et buk y Solanum tuberosum

    Directory of Open Access Journals (Sweden)

    Caro Muñoz Marina

    2004-12-01

    Full Text Available Esta investigación se realizó en el Laboratorio de Cultivo de Tejidos Vegetales del Instituto de Biotecnología de la Universidad Nacional de Colombia (IBUN y en los invernaderos de la Facultad de Agronomía, Sede Bogotá. El propósito fue evaluar una metodología para propagación
    de plántulas y producción de tubérculo semilla élite en cuatro variedades de papa: Solanum tuberosum L. ssp andígena (Diacol-Capiro, ICA-Puracé y Parda Pastusa y Solanum phureja Juz et Buk. (“Yema de huevo”-Clon 1, a partir de esquejes obtenidos de plantas provenientes de cultivos
    in vitro libres de virus, indexadas mediante prueba de ELISA. Una vez que las plantas eran adaptadas a condiciones ex vitro y alcanzaron una longitud de más de 25 cm, se cortaron esquejes que portaban una yema apical con dos nudos y el esqueje axilar siguiente con la yema y su
    hoja. La base de éstos se sumergió en soluciones de ácido naftalenacético (ANA en concentraciones de 0 (control, 5, 10 y 15 mg/l durante una hora, después fueron colocados en bandejas de enraizamiento que contenían turba como soporte. También se utilizó un producto comercial (Hormonagro No.1 cuyo ingrediente activo es ANA al 0,04% para la inducción de raíces en ambos tipos de esqueje como un ensayo adicional comparándolo con un control (sin aplicación de enraizador; los esquejes se impregnaron con el polvo en su base e inmediatamente se pusieron en las bandejas de enraizamiento. Se hicieron ensayos independientes con cada variedad para evaluar los efectos de las dosis de ANA y Hormonagro sobre los dos tipos de esqueje
    (axilar y apical. Se evaluaron las siguientes variables respuesta: porcentaje de supervivencia de esquejes a la tercera semana, longitud y número de hojas de esquejes durante tres semanas, área foliar y peso seco de esquejes a la cuarta semana. Después de esto los esquejes se sembraron en bolsas plásticas de 10 x 14 pulgadas calibre seis y se llevaron a

  7. 甘肃省马铃薯束梗褐腐病病原鉴定及其生物学特性研究%Identification and biological characteristics of Solanum tuberosum brown rot caused by Doratomyces stemonitis

    Institute of Scientific and Technical Information of China (English)

    陈泰祥; 陈秀蓉; 杨成德; 朱海波; 王涵琦; 卞静

    2014-01-01

    The identification and biological characteristics of the pathogen isolated from brown rot of Solanum tu-berosum were studied.The pathogen was identified as Doratomyces stemonitis (Pers.ex Fr.)F.J.Morton &G. Smith based on morphological characteristics as well as rDNA-ITS sequences.The optimum temperature for myce-lial growth was 25-30 ℃ at pH 6.The pathogen could use monosaccharide,disaccharide and polysaccharide;the best carbon source was sucrose,and the best nitrogen source was glycine.Illumination had no obvious promotion effect on mycelial growth.The optimum temperature for sporangium sprout was 25 ℃ at pH 7.Spores could ger-minate only in water drop.The filtrate of potato and glucose solution had obvious promotion effect on spore ger-mination.Soil solution had no effects on spore germination.%对甘肃省马铃薯(Solanum tuberosum L.)束梗褐腐病病原进行了分离鉴定和生物学特性研究。经形态特征观察与 rDNA-ITS 序列分析,将病原菌鉴定为细基束梗霉[Doratomyces stemonitis (Pers.ex Fr.)F.J.Morton &G.Smith]。生物学特性研究结果表明,病菌菌丝生长最适温度为25~30℃,最适 pH 为6,病菌能利用多种碳源,但以蔗糖最好,氮源以甘氨酸最适,光照对菌丝生长没有影响。分生孢子在5~40℃范围内均能萌发,最适25℃,最适 pH 为7,分生孢子萌发需液态水,湿度低于99%几乎不萌发,马铃薯汁液和葡萄糖液对孢子萌发有较好的促进作用。本研究为马铃薯束梗褐腐病的防治提供了理论依据。

  8. Caracterização química e física de batatas ágata e monalisa minimamente processadas Chemical and physical characterization of fresh-cut potatoes

    Directory of Open Access Journals (Sweden)

    Lívia L. O. Pineli

    2006-03-01

    Full Text Available O objetivo deste trabalho foi caracterizar química e fisicamente batatas minimamente processadas durante o armazenamento refrigerado. Batatas (Solanum tuberosum, L. ágata e monalisa foram minimamente processadas como minibatatas. Após o processamento, as batatas foram acondicionadas sob vácuo parcial e, posteriormente, armazenadas em câmaras frias a 5 e 15ºC, por nove dias. A cada três dias, foram avaliadas as seguintes variáveis: firmeza, atividade da polifenoloxidase e peroxidase, açúcares solúveis totais, amido e vitamina C total. Nas batatas armazenadas a 15ºC, constatou-se que, após nove dias de armazenamento, sua firmeza era 3,3 vezes menor em batatas monalisa e 4,3 vezes menor para a cultivar ágata, quando comparadas com o produto recém-processado. A atividade da polifenoloxidase mostrou-se praticamente estável em batatas monalisa armazenadas a 5ºC. Batatas monalisa minimamente processadas apresentaram maior atividade da peroxidase a 5ºC, sendo 86% maior do que a atividade desta enzima em batatas ágata ao final do período experimental. O teor inicial de açúcares solúveis totais nas batatas minimamente processadas era 28% maior na cultivar monalisa, quando comparada com tubérculos de ágata. As duas cultivares apresentaram tendência de elevação do teor de amido nos primeiros três dias, para as duas temperaturas estudadas. Em ambas as cultivares, armazenamento a 5ºC possibilitou maior manutenção dos teores de vitamina C.The present work was carried out aiming to evaluate chemical and physical characteristics of fresh-cut potatoes stored under 5 and 15°C. Potatoes, (Solanum tuberosum L. ágata and Monalisa were minimally processed as baby potatoes. After processing, the material was packed under partial vacuum and stored at 5 and 15°C for nine days. Every three days tubers were evaluated for firmness, enzymatic activity (polyphenoloxidase and peroxidase, total soluble sugars, starch, and total vitamin C

  9. Production of Phytophthora infestans-resistant potato (Solanum tuberosum) utilising Ensifer adhaerens OV14

    DEFF Research Database (Denmark)

    Wendt, Toni; Doohan, Fiona; Mullins, Ewen

    2012-01-01

    Arabidopsis thaliana (0.12%) and potato (mean transformation frequency 35.1%). Thereafter, Ensifer adhaerens was used to generate blight- (causative organism Phytophthora infestans) resistant potato using the Solanum bulbocastanum ‘resistance to blight’ (RB) gene. Resistant genotypes were confirmed by...

  10. Guatemalan potato moth Tecia solanivora distinguish odour profiles from qualitatively different potatoes Solanum tuberosum L.

    DEFF Research Database (Denmark)

    Karlsson, Miriam Frida; Birgersson, Göran; Witzgall, Peter;

    2013-01-01

    Guatemalan potato moth, Tecia solanivora, lay eggs in the soil nearby potato Solanum spp. and larvae feed on the tubers. We investigated the oviposition behaviour of T. solanivora females and the survival of larval offspring on healthy vs. stressed, i.e. light exposed and/or damaged potato tubers...

  11. Pólen 2n e mecanismos meióticos de formação em Solanum commersonii ssp 2n pollen and meiotic mechanisms of Formation in Solanum commersonii ssp

    Directory of Open Access Journals (Sweden)

    Lívia Gracielle Oliveira Tomé

    2009-04-01

    Full Text Available Solanum commersonii subsp. commersonii Dun. e Solanum commersonii subsp. malmeanum Bitt. (2n=2x=24 - 1EBN não podem ser diretamente cruzadas com a batata cultivada de S. tuberosum subsp. tuberosum L. (2n=4x=48 -4 EBN em razão da barreira da ploidia e do número de equilíbrio do endosperma (EBN. No entanto, a produção de pólen não reduzido por S. commersonii ssp. (2x=2n=24 - 1EBN, permite o cruzamento desta com dihaplóides de batata e com as espécies diplóides S. phureja Juz. & Bukasov. e S. chacoense Bitt., que possuem 2 EBN. Conduziu-se este trabalho, com o objetivo de determinar o mecanismo meiótico e a freqüência dos grãos de pólen não reduzidos em cinco clones de Solanum commersonii ssp. Foram avaliados três clones de S. commersonii subsp. commersonii (SCC e dois de S. commersonii subsp. malmeanum (SCM. Nas avaliações, duzentos grãos de pólen viáveis foram corados (carmim acético 2% e mensurados, sendo considerados não reduzidos aqueles com o diâmetro igual ou superior a 25 µm. O clone Solanum commersonii subsp. commersonii Dun. (SCC 100 produz 26.1% de polen não reduzido, por fusos fundidos e fusos paralelos, o qual pode ser utilizado em programas de melhoramento.Solanum commersonii subsp. commersonii Dun. and Solanum commersonii subsp. malmeanum Bitt. (2n=2x=24 -1EBN cannot be directly crossed with the cultivated Solanum tuberosum subsp. Tuberosum L. (2n=4x=48 - 4EBN species due to ploidy barriers and endosperm balance number (EBN. However, non-reduced pollen production by the wild species S. commersonii ssp. (2x=2n=24 -1EBN, allows the crossing of these species with dihaploids of potato and with the diploids species, S. phureja Juz. & Bukasov. and S. chacoense Bitt., all with 2EBN. The aim of this work was to determine the meiotic mechanism and frequency of non-reduced pollen production of five clones of S. commersonii ssp. Three different clones of S. commersonii subsp. commersonii Dun. (SCC and two of S

  12. Ocorrência de glicoalcalóides e esverdeamento em tubérculos de batata recém-colhidos e armazenados Occurrence of glycoalkaloids and greening in newly harvested and stored potato tubers

    OpenAIRE

    Dayse S. Spoladore; João Paulo F. Teixeira; Marco Antônio T. Zullo; Paulo R. M. Teixeira; Sônia M. B. M. Coelho; Hilário S. Miranda Filho

    1983-01-01

    Foram analisados 36 dos cultivares de batata (Solanum tuberosum L.) existentes no Instituto Agronômico, quanto ao teor de glicoalcalóides totais (TGA) na porção superficial dos tubérculos, e quanto à sua capacidade de esverdeamento, duas características importantes na comercialização do produto. As determinações foram feitas para tubérculos recém-colhidos, armazenados na ausência e na presença de luz, ambos por 25 dias. Os teores de TGA situaram-se na faixa de 3-24mg/100g de peso fresco. Tant...

  13. Sustainable Agriculture and Climate Change: Producing Potatoes (Solanum tuberosum L. and Bush Beans (Phaseolus vulgaris L. for Improved Food Security and Resilience in a Canadian Subarctic First Nations Community

    Directory of Open Access Journals (Sweden)

    Christine D. Barbeau

    2015-05-01

    Full Text Available Aboriginal people in Canada experience disproportionately high rates of diet-related illnesses, such as obesity and diabetes. Food insecurity has been identified as a contributing factor to these illnesses along with a loss of traditional lifestyle. Current food systems within northern subarctic and arctic regions of Canada rely heavily on imported foods that are expensive (when available, and are environmentally unsustainable. A warming subarctic and arctic climate present challenges, but also offers the opportunity for local agricultural production that can increase food security and promote a more sustainable food system. In this study the feasibility of sustainably growing potatoes (Solanum tuberosum L. utilizing agroforestry practices to enhance food security in remote subarctic communities is explored through a case study in Fort Albany First Nation in northern Ontario, Canada. Potato crops were grown over a two-year period and rotated into plots that had been planted with green bush beans (Phaseolus vulgaris L.. Results showed that potatoes and bush beans could be grown successfully in the subarctic without the use of greenhouses with yields comparable to more conventional high-input agricultural methods. In subarctic Canada, sustainable local food production can help to promote social capital, healthier lifestyles, and food security.

  14. Impact of the nutrients N and K and soluble sugars on Diabrotica speciosa (Germar) (Coleoptera, Chrysomelidae) and Agrotis ipsilon (Hufnagel) (Lepidoptera, Noctuidae) populations in potato crops, Solanum tuberosum L. (Solanaceae)

    International Nuclear Information System (INIS)

    Impact of the nutrients N and K and soluble sugars on Diabrotica speciosa (Germar) (Coleoptera, Chrysomelidae) and Agrotis ipsilon (Huefnagel) (Lepidoptera, Noctuidae) populations in potato crops, Solanum tuberosum L. (Solanaceae). The occurrence of Diabrotica speciosa (Germar, 1824) and Agrotis ipsilon (Huefnagel, 1767) on the potato cultivars Achat and Monalisa, influenced by nitrogen and potassium dosage, and minimum quantity of soluble sugars, was studied. The following parameters were evaluated: concentration of mineral nutrient and sugar in green leaf, senescent leaf, leaf in abscission, stem, tubercle and total plant using extracts of infusion in ethanol 80%. The largest infestation of D. speciosa larvae was on Monalisa cultivar at 150 kg.ha-1 of N + K with 27.03% at P-1, in the absence of potassium. On the other hand, high dosage of K reduced the damages by A. ipsilon on Monalisa cultivar. However, it did not influence the storage of soluble sugar. The results indicated that in Achat cultivar the accumulated soluble sugar was reduced, probably sensitized by elevation of potassic fertilization dosing, differing from Monalisa cultivar, in which the influence was by nitrogen dosing. (author)

  15. 干旱胁迫对马铃薯品种生理特性的影响%Effects of Drought Stress on Physiological Characteristics of Potato (Solanum tuberosum L.)

    Institute of Scientific and Technical Information of China (English)

    海梅荣; 陈勇; 周平; 王丽; 刘福翠; 唐天向; 达布希拉图

    2014-01-01

    干旱是最严重的自然灾害之一,影响植物的生长、发育和繁殖等生命活动。干旱逆境可引起植物生理生化方面的一系列变化。近几年,云南省干旱问题愈加严重,因此,本研究以云南省主栽马铃薯(Solanum tuberosum L.)品种‘会-2’为试验材料,研究干旱胁迫对马铃薯抗氧化酶活性和光合特性的影响,以期在干旱情况下为稳定马铃薯的生产、提高产量提供理论依据。结果表明,随着干旱胁迫的加剧块茎干重下降比较明显;在光合特性方面,净光合速率、气孔导度、蒸腾速率和细胞胞间隙CO2浓度降低,干旱胁迫下,细胞失水,气孔关闭,限制CO2吸收,蒸腾速率减弱,从而影响光合作用的速率;干旱胁迫会诱导抗氧化酶活性的降低,减弱抗氧化酶对活性氧的清除能力,导致危害植物的丙二醛大量积累。%Drought stress is one of the most serious natural disasters, affecting the growth, development and reproduction of plants. It could also lead to the plant physiological and biochemical changes. Drought in Yunnan Province occurs more frequently in recent years, and an experiment was designed thereafter using potato (Solanum tuberosum L.) variety‘Hui-2’as experimental material to investigate the effects of drought stress on the activity of antioxidant enzymes and photosynthetic characteristics in potato, which might provide a theoretical base for stable and high yielding production of potato in a drought environment. The results showed that yield decreased with the drought stress deteriorating. Also, the net photosynthetic rate in leaf reduced due to the decline in stomatal conductance, transpiration rate and intercellular CO2 concentration. The results indicated that drought stress reduced antioxidant enzyme activity, and its scavenging capacity of active oxygen, resulting in a large number of malondialdehyde, which might harm the potato plants.

  16. The futile cycling of hexose phosphates could account for the fact that hexokinase exerts a high control on glucose phosphorylation but not on glycolytic rate in transgenic potato (Solanum tuberosum roots.

    Directory of Open Access Journals (Sweden)

    Eric Claeyssen

    Full Text Available The metabolism of potato (Solanum tuberosum roots constitutively over- and underexpressing hexokinase (HK, EC 2.7.1.1 was examined. An 11-fold variation in HK activity resulted in altered root growth, with antisense roots growing better than sense roots. Quantification of sugars, organic acids and amino acids in transgenic roots demonstrated that the manipulation of HK activity had very little effect on the intracellular pools of these metabolites. However, adenylate and free Pi levels were negatively affected by an increase in HK activity. The flux control coefficient of HK over the phosphorylation of glucose was measured for the first time in plants. Its value varied with HK level. It reached 1.71 at or below normal HK activity value and was much lower (0.32 at very high HK levels. Measurements of glycolytic flux and O(2 uptake rates demonstrated that the differences in glucose phosphorylation did not affect significantly glycolytic and respiratory metabolism. We hypothesized that these results could be explained by the existence of a futile cycle between the pools of hexose-Ps and carbohydrates. This view is supported by several lines of evidence. Firstly, activities of enzymes capable of catalyzing these reactions were detected in roots, including a hexose-P phosphatase. Secondly, metabolic tracer experiments using (14C-glucose as precursor showed the formation of (14C-fructose and (14C-sucrose. We conclude that futile cycling of hexose-P could be partially responsible for the differences in energetic status in roots with high and low HK activity and possibly cause the observed alterations in growth in transgenic roots. The involvement of HK and futile cycles in the control of glucose-6P metabolism is discussed.

  17. Detección del virus del amarillamiento de las nervaduras de la hoja de la papa en diferentes órganos de Solanum tuberosum grupo Phureja cv Criolla Colombia utilizando RT-PCR convencional y en tiempo real

    Directory of Open Access Journals (Sweden)

    Anngie Katherine Hernández-Guzmán

    2014-06-01

    Full Text Available Título en ingles: Potato yellow vein virus detection in different organs of Solanum tuberosum Phureja group cv Criolla Colombia by conventional and real time qRT-PCRTitulo corto: Detección del virus PYVVV en diferentes órganos de Solanum tuberosum grupo Phureja Resumen: La producción del cultivo de papa en Colombia se puede afectar por infección con diferentes patógenos virales, entre ellos, el Potato yellow vein virus (PYVV que puede reducir la producción entre el 30 % y 50%. PYVV se ha diagnosticado molecularmente usando RT-PCR convencional en hojas sintomáticas y no sintomáticas. Sin embargo, no hay reportes sobre la detección y distribución viral en diferentes órganos infectados por PYVV en las plantas que expresan síntomas y sin síntomas. El objetivo de esta investigación, fue detectar a PYVV por RT-PCR convencional con cebadores específicos y por qRT-PCR (tiempo real utilizando Sondas TaqMan® y analizar la distribución viral en plantas de S. tuberosum grupo Phureja cv. Criolla Colombia (papa criolla. Se logró la detección del virus en todos los órganos analizados (foliolo, peciolo, tallo aéreo y subterráneo, pedúnculo floral, pétalo y antera mediante ambas técnicas, sin embargo, qRT-PCR fue 100 veces más sensible que la técnica convencional. Adicionalmente, se realizó la cuantificación absoluta del gen de la proteína mayor de la cápside de PYVV (CP. Los resultados indican que cuando la planta no expresa síntomas (NS, hay una distribución homogénea del virus, con un promedio del número de copias del gen CP de 4.09107±2.35107; mientras que en plantas sintomáticas el título viral es mayor (6.82108±1.74108 y la distribución heterogénea en los órganos, con mayor acumulación en órganos de la zona aérea. Este es el primer informe sobre la  detección de PYVV en diferentes órganos de papa por medio de tiempo real,  incluyendo las anteras y pedúnculo floral. La información debe ser de utilidad

  18. Ascorbic Acid and/or 24-Epibrassinolide Trigger Physiological and Biochemical Responses for the Salt Stress Mitigation in Potato (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Chandrama Prakash Upadhyaya

    2015-12-01

    Full Text Available In the present study, we examined the role of ascorbic acid (AsA, vitamin C and/or 24-epibrassinolide (EBL, an active BR in mitigation of salt-induced stress in potato (Solanum tuberousum L. The 10-d-old plants were exposed to 150 mM NaCl and they were subsequently treated by ASA and/or EBL. The salt stress reduced significantly the plant growth, tuber yield, total chlorophyll and increased proline content and electrolyte leakage in the leaves. Toxic effects induced by salt stress were completely overcome by the combined exogenous application of AsA and EBL. The AsA and/or EBL treatments improved the growth parameters of the salt treated plants, such as shoot length, tuber number and size, fresh and dry mass and other physiological parameters. Our data also indicated that applications of AsA and EBL up-regulated the stress regulating plant hormone such as IAA, IBA and activities of the antioxidant enzymes, such as catalase (CAT, peroxidase (POX, superoxide dismutase (SOD, ascorbate peroxidase (APX and under salt stress.

  19. Asociaciones de marcadores moleculares con la resistencia a enfermedades, caracteres morfológicos y agronómicos en familias diploides de papa (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Julio Gabriel

    2016-05-01

    Full Text Available Fefteen families of potato (840 genotypes originated from inter-specific crosses among Solanum stenotomum, S. goniocalyx and S. phureja were genotyped in order to associate six molecular markers (GP94, HC, Nl25, Gro 1-4, RYSC3 y CP60. These molecular markers are linked to major genes for resistance to late blight (Phytophthora infestans, wart (Synchytrium endobioticum, root cyst nematode (Globodera pallida and G. rostochiensis and viruses PVY and PVX. Results showed that five of six molecular markers were polymorphic and primers amplified in more than 80 % of the families. Molecular marker RYSC3 that is linked to Ryadg gene did not amplify in any family. All individuals in Family 8 scored positive for three markers (CP60, GP94, and NL25. χ2 test was used to determine the significance of the segregation ratios of each marker in every single family; so to the possible genotype of its parents was inferred. Nine morphological and agronomical characters were assessed during the harvest. Cluster analysis classified and selected 107 genotypes by putative resistance to PVX, P. infestans, G. rostochiensis and S. endobioticum, high yield, tuber volume and tuber number and shallow eyes. According to our results, practical aspects of efficient use of marker assisted selection in potato are discussed in this paper.

  20. Técnica para o estudo da anatomia da epiderme foliar de batata A technique for the anatomical study of potato leaf epidermis

    Directory of Open Access Journals (Sweden)

    Fernanda Bastos Segatto

    2004-10-01

    Full Text Available Embora a batata (Solanum tuberosum seja uma espécie de grande valor econômico, são poucas as informações disponíveis sobre a anatomia foliar, principalmente de características estruturais da epiderme. O estudo da anatomia foliar assume grande importância básica, para a ecologia e fisiologia, e aplicada para a fitopatologia e o melhoramento genético. Este trabalho teve por objetivo identificar uma técnica histológica que permite a confecção de lâminas semipermanentes para avaliar a epiderme foliar, em vista frontal, de um grande número de plantas de batata em um curto período de tempo. O corte paradérmico a mão livre, o método de Jeffrey e as técnicas de hidróxido de potássio e de impressão da epiderme foram avaliados. Lâminas semipermanentes foram confeccionadas da secção frontal da epiderme de folíolos principais do terço médio de plantas de batata. A impressão da epiderme em lâmina foi a única técnica que possibilitou a avaliação adequada de todos os clones de batata utilizados. A impressão da epiderme é uma técnica muito rápida, de baixo custo e de fácil execução, que atende os requisitos necessários para a identificação e seleção de características anatômicas de um grande número de plantas em um programa de melhoramento genético de batata.Even though potato (Solanum tuberosum is an economically important crop, information about leaf characteristics of anatomical structure has not been well studied. Studies of leaf epidermis have significance for basic fields as ecology and physiology and for applied fields as plant pathology and breeding. The objective of this research was to identify a fast and easy technique to prepare semi-permanent slides for studying the anatomy of potato leaf epidermis. Para dermal free hand cut, Jeffrey method, potassium hydroxide and epidermal fingerprint on glass slide techniques were evaluated. Semi-permanent slides were prepared from the longitudinal section of

  1. Expresión de la proteína Cry1Ac en tejidos de líneas transgénicas de papa (Solanum tuberosum spp. Andígena Var. Diacol capiro.

    Directory of Open Access Journals (Sweden)

    Blanco Martinez Jennifer Teresa

    2010-08-01

    Full Text Available

    RESUMEN

    La papa (Solanum sp. es el cuarto producto alimenticio más importante en el mundo. En Colombia anualmente se producen alrededor de 2,8 millones de toneladas, sirviendo como sustento económico a 90.000 familias. En el país, Tecia solanivora genera el mayor impacto económico en el cultivo con pérdidas de hasta el 100% en la producción de tubérculos. El fitomejoramiento vía introducción de genes Cry, que codifican para cristales proteicos insecticidas, constituye una alternativa para reducir el ataque de insectos en cultivos de interés comercial. En este trabajo se caracterizó la inserción, transcripción y expresión del gen Cry1Ac en diferentes tejidos y en tres etapas del desarrollo para dos líneas transgénicas de Solanum tuberosum spp. andígena variedad Diacol Capiro. La caracterización se realizó a través de técnicas de PCR, RT-PCR y ELISA. Se corroboró la inserción y transcripción del gen utilizando primers que amplificaron una banda específica de 766pb para Cry1Ac. Los niveles de expresión de la proteína llegaron a ser mayores a 45μg/g

  2. Transfer of U, Al and Mn in the water-soil-plant (Solanum tuberosum L.) system near a former uranium mining area (Cunha Baixa, Portugal) and implications to human health

    Energy Technology Data Exchange (ETDEWEB)

    Neves, M.O., E-mail: orquidia.neves@ist.utl.pt [Centro de Petrologia e Geoquimica, Instituto Superior Tecnico, Universidade Tecnica de Lisboa (TULisbon), Av. Rovisco Pais 1049-001 Lisboa (Portugal); Figueiredo, V.R., E-mail: vera.figueiredo@ist.utl.pt [Instituto Superior Tecnico, Universidade Tecnica de Lisboa (TULisbon), Av. Rovisco Pais 1049-001 Lisboa (Portugal); Abreu, M.M., E-mail: manuelaabreu@isa.utl.pt [Unidade de Investigacao de Quimica Ambiental, Instituto Superior de Agronomia, Universidade Tecnica de Lisboa (TULisbon), Tapada da Ajuda, 1349-017 Lisboa (Portugal)

    2012-02-01

    Knowledge about metals in crops, grown in contaminated soils around mine sites, is limited and concerns about exposure to hazardous elements through the consumption of contaminated foodstuff, are high. In this study a field experiment was carried out in two agricultural soils located near a former uranium mine area (Cunha Baixa, Portugal). The purpose of the study was to assess the effect of irrigation water quality on soil-potato (Solanum tuberosum L.) crop system and to evaluate if the consumption of the crop represents health risk to the local villagers. The soils were divided in two plots: one irrigated with contaminated water (U: 1.03-1.04 mg/L; Al: 7.5-8.00 mg/L; Mn: 4.52 mg/L) and the other with uncontaminated water (U: 14-10 {mu}g/L; Al: 17-23 {mu}g/L; Mn: 2.4-5.7 {mu}g/L). After irrigation and potato growth, only soil characteristics, as salinity and total U and Mn concentrations were significantly different from those measured at the beginning of the experiment. Within the potato plants, elements were mostly translocated and concentrated in the aerial part: stems and leaves (U: 73-87%; Al: 85-96%; Mn: 85-94%), which minimize the risk of contamination of the edible tissue. In potato tubers, the highest average concentrations (121-590 {mu}g U/kg; 25-64 mg Al/kg; 12-13 mg Mn/kg dry weight) were registered at soil plots irrigated with contaminated water. Uranium and Al were mostly concentrated in the potato peel (88-96 and 76-85%, respectively), and Mn (67-78%) in the pulp, which reinforces the importance of removing peel to minimize human exposure. The risk analysis calculated for non-cancer health effects (hazard quotient), related only to the exposure through the consumption of this basic foodstuff, revealed safety for Cunha Baixa village residents (adults and children) even when potato crop was grown on U enriched soils and irrigated with contaminated water. - Highlights: Black-Right-Pointing-Pointer Field experiment with potato in agricultural soils near

  3. Residuos de plaguicidas en la cáscara e interior de la papa (Solanum tuberosum L. proveniente de una región agrícola del estado Mérida, Venezuela

    Directory of Open Access Journals (Sweden)

    Pedro Benítez-Díaz

    2015-04-01

    Full Text Available Dada la naturaleza tóxica de los plaguicidas y a su constante aplicación en cultivos agrícolas destinados al consumo humano, el objetivo del presente trabajo fue determinar la presencia de residuos plaguicidas en la cáscara y la porción interna de la papa (Solanum tuberosum L., cosechadas en el Municipio Rivas Dávila del estado Mérida, Venezuela. Para el análisis, se utilizó el método SPE-HPLC-DAD. En la cáscara se detectaron ocho plaguicidas: clorpirifos (7,3 mg·kg-1, diazinon (11,8 mg·kg-1, dimetoato (0,56 mg·kg-1, metamidofos (5,0 mg·kg-1, carbofuran (1,4 mg·kg-1, mancozeb (11,4 mg·kg-1, metomilo (0,030 mg·kg-1 y metribuzin (0,10 mg·kg-1, todos ellos por encima del límite máximo de residuos (LMR establecidos por el Codex Alimentarius y la Comunidad Europea. En la porción interna se detectaron residuos de cinco plaguicidas considerados potentes neurotóxicos y disruptores endocrinos: clorpirifos (13 mg·kg-1, diazinon (5,2 mg·kg-1, metamidofos (4,5 mg·kg-1, carbofuran (1,13 mg·kg-1 y mancozeb (2,51 mg·kg-1, todos por encima del LMR. Llama particularmente la atención las concentraciones extremadamente altas del diazinon y clorpirifos; este último parece concentrarse en el interior del tubérculo. Se recomienda eliminar la cáscara antes de consumir o procesar la papa, particularmente porque en el interior del tubérculo disminuye la frecuencia de detección y casi todos los plaguicidas disminuyen su concentración. Estos resultados demuestran que el uso inadecuado de plaguicidas en cultivos de papa podría afectar negativamente la calidad del tubérculo, poniendo en riesgo la salud de los consumidores finales del producto.

  4. Transfer of U, Al and Mn in the water–soil–plant (Solanum tuberosum L.) system near a former uranium mining area (Cunha Baixa, Portugal) and implications to human health

    International Nuclear Information System (INIS)

    Knowledge about metals in crops, grown in contaminated soils around mine sites, is limited and concerns about exposure to hazardous elements through the consumption of contaminated foodstuff, are high. In this study a field experiment was carried out in two agricultural soils located near a former uranium mine area (Cunha Baixa, Portugal). The purpose of the study was to assess the effect of irrigation water quality on soil–potato (Solanum tuberosum L.) crop system and to evaluate if the consumption of the crop represents health risk to the local villagers. The soils were divided in two plots: one irrigated with contaminated water (U: 1.03–1.04 mg/L; Al: 7.5–8.00 mg/L; Mn: 4.52 mg/L) and the other with uncontaminated water (U: 14–10 μg/L; Al: 17–23 μg/L; Mn: 2.4–5.7 μg/L). After irrigation and potato growth, only soil characteristics, as salinity and total U and Mn concentrations were significantly different from those measured at the beginning of the experiment. Within the potato plants, elements were mostly translocated and concentrated in the aerial part: stems and leaves (U: 73–87%; Al: 85–96%; Mn: 85–94%), which minimize the risk of contamination of the edible tissue. In potato tubers, the highest average concentrations (121–590 μg U/kg; 25–64 mg Al/kg; 12–13 mg Mn/kg dry weight) were registered at soil plots irrigated with contaminated water. Uranium and Al were mostly concentrated in the potato peel (88–96 and 76–85%, respectively), and Mn (67–78%) in the pulp, which reinforces the importance of removing peel to minimize human exposure. The risk analysis calculated for non-cancer health effects (hazard quotient), related only to the exposure through the consumption of this basic foodstuff, revealed safety for Cunha Baixa village residents (adults and children) even when potato crop was grown on U enriched soils and irrigated with contaminated water. - Highlights: ► Field experiment with potato in agricultural soils near a

  5. 不同贮藏条件下马铃薯块茎皮中龙葵素含量的变化%Content of Solanine in Tuber Peel of Potato (Solanum tuberosum L.) Under Different Storage Conditions

    Institute of Scientific and Technical Information of China (English)

    张薇; 邱成; 高荣; 熊兴耀

    2013-01-01

      马铃薯块茎在不同温度和光照条件下贮藏6~18 d,用乙醇-乙酸法提取块茎皮中的α-茄碱,用高效液相色谱法测定其含量,研究块茎皮中龙葵素含量的变化。结果表明,在有光照、贮藏温度为8,15,25℃条件下贮藏6 d,样品中α-茄碱的含量分别为1.35%,4.96%和9.67%;在有光照、贮藏温度15℃的条件下,贮藏时间为6,12,18 d,样品中α-茄碱的含量分别为4.96%,8.78%和12.76%;在25℃、贮藏时间6 d 条件下,有光照和无光照处理的样品中α-茄碱含量分别为9.67%和7.61%。块茎皮中的α-茄碱含量随贮藏温度和时间的增加而增加,光照条件下块茎皮中的α-茄碱含量明显增加。%Changes in solanine content in the tuber peel of potato (Solanum tuberosum L.) were studied under different temperature and light conditions and storage periods. Solanine was extracted by ethanol-acetic acid, and measured by High Performance Liquid Chromatography. The contents of solanine in peel of tuber under the store temperature of 8, 15 and 25 ℃ for 6 days combined with light il umination were, respectively, 1.35%, 4.96% and 9.67%. The contents of solanine in skin of tuber under the storage period of 6, 12 and 18 d at 15℃ with light were, respectively, 4.96%, 8.78%, and 12.76%. The contents of solanne in skin of tuber stored at 25℃ for 6 d with and without light were, respectively, 9.67% and 7.61%. The contents of solanne in skin of tuber increased with storage temperature and period, and noticeably increased under light.

  6. Transfer of U, Al and Mn in the water-soil-plant (Solanum tuberosum L.) system near a former uranium mining area (Cunha Baixa, Portugal) and implications to human health.

    Science.gov (United States)

    Neves, M O; Figueiredo, V R; Abreu, M M

    2012-02-01

    Knowledge about metals in crops, grown in contaminated soils around mine sites, is limited and concerns about exposure to hazardous elements through the consumption of contaminated foodstuff, are high. In this study a field experiment was carried out in two agricultural soils located near a former uranium mine area (Cunha Baixa, Portugal). The purpose of the study was to assess the effect of irrigation water quality on soil-potato (Solanum tuberosum L.) crop system and to evaluate if the consumption of the crop represents health risk to the local villagers. The soils were divided in two plots: one irrigated with contaminated water (U: 1.03-1.04mg/L; Al: 7.5-8.00mg/L; Mn: 4.52mg/L) and the other with uncontaminated water (U: 14-10μg/L; Al: 17-23μg/L; Mn: 2.4-5.7μg/L). After irrigation and potato growth, only soil characteristics, as salinity and total U and Mn concentrations were significantly different from those measured at the beginning of the experiment. Within the potato plants, elements were mostly translocated and concentrated in the aerial part: stems and leaves (U: 73-87%; Al: 85-96%; Mn: 85-94%), which minimize the risk of contamination of the edible tissue. In potato tubers, the highest average concentrations (121-590μg U/kg; 25-64mg Al/kg; 12-13mg Mn/kg dry weight) were registered at soil plots irrigated with contaminated water. Uranium and Al were mostly concentrated in the potato peel (88-96 and 76-85%, respectively), and Mn (67-78%) in the pulp, which reinforces the importance of removing peel to minimize human exposure. The risk analysis calculated for non-cancer health effects (hazard quotient), related only to the exposure through the consumption of this basic foodstuff, revealed safety for Cunha Baixa village residents (adults and children) even when potato crop was grown on U enriched soils and irrigated with contaminated water. PMID:22178025

  7. SSR标记的彩色马铃薯遗传多样性分析及指纹图谱构建%Genetic Diversity and Fingerprinting of 50 Pigmented Potato (Solanum tuberosum L.) Genetypes with SSR Markers

    Institute of Scientific and Technical Information of China (English)

    石景; 宋波涛; 金开建; 柳俊

    2012-01-01

    Pigments of potato tubers have been suggested the benefits for human beings health and have attracted interests in science which approach to their genetic improvement, and in fresh and industrial markets which have potentials of added-values. Little information is available for diversity of pigmented potato germplasms resulted in a bottle-neck of selection and enhancement of suitable parental materials for the potato breeding. In present research, SSR markers were employed to clarify the genetic diversity and to establish the fingerprinting of 50 pigmented potato (Solanum tuberosum L.) genotypes which were available in existed breeding programs. The results showed that 56 pairs of SSR primers having polymorphism were selected by screening over 200 SSR markers. A total of 236 alleles were identified, of which 230 were polymorphic and the ratio of polymorphism was as high as 97.46%, which indicated that the SSR markers selected were suitable for the genetic diversity clarification. The genetic similarity of the 50 tested materials ranged from 0.50 to 1.00, suggesting relative narrow genetic resources which they were derived from. The UPGMA cluster analysis indicated that all the materials could be assigned into three groups at genetic similarity' of 0.63. Cluster I contained 11 genotypes, 38 genotypes were assigned to ClusterⅡ while Cluster Ⅲ had only one genotype. Cluster Ⅰ was assigned into 2 subgroups at genetic similarity of 0.64, and cluster Ⅱ was assigned into 3 subgroups at genetic similarity of 0.67. Five pairs of SSR primer were successfully used to establish DNA fingerprints of the 50 tested materials by detecting 32 alleles. Marker StI005 alone could identify 32 genotypes, bi-marker combination of StI005/StI007 could detect 43 genotypes and tri-marker combination of StI005/StI007/S038 could distinguish 46 genotypes. The rest four cultivars, British Columbia and Congo as well as Macintosh Black and Black Beauty, could be further identified by S072

  8. Quebra de dormência de minitubérculos de batata Dormancy breaking of potato minitubers

    Directory of Open Access Journals (Sweden)

    Marlova Benedetti

    2005-02-01

    Full Text Available A quebra de dormência de batata-semente (Solanum tuberosum é necessária em programas de melhoramento genético, na multiplicação de tubérculos livres de vírus e na produção comercial de batata quando o período entre a colheita e o próximo plantio é insuficiente para a brotação dos tubérculos. Um experimento foi conduzido com o objetivo de avaliar a eficácia de diferentes tratamentos para a quebra de dormência de minitubérculos de batata dos clones Macaca, de curta dormência, e SMIJ461-1, de longa dormência. Os tratamentos foram: testemunha, aspersão com etanol e ácido giberélico (10mg L-1, abafamento com bissulfureto de carbono (35mm m-3 por 72h, aspersão com ácido giberélico (30mg L-1 seguido de abafamento por 72h, imersão com ácido 2-4 cloroetil fosfônico (840mg L-1 por 5s, e alternância de temperaturas (ciclos de sete dias a 4ºC e sete dias a 20ºC. O delineamento experimental foi um trifatorial (clone x quebra de dormência x safra no delineamento experimental inteiramente casualizado, com quatro repetições de dez minitubérculos. A resposta aos tratamentos variou entre clones e safras. A maior resposta dos tratamentos para a quebra de dormência foi com o clone Macaca. Os tratamentos com ácido giberélico foram os mais eficazes para a quebra de dormência de ambos os clones. A percentagem final de minitubérculos brotados do clone SMIJ461-1 foi baixa, necessitando de maiores estudos quanto à concentração e tempo de aplicação dos tratamentos.In some potato (Solanum tuberosum grown areas, time between harvesting and planting is shorter than seed dormancy period. In these areas, dormancy breaking is necessary in breeding programs, virus-free seed production and commercial production fields. An experiment was carried out with the objective of evaluating the efficacy of different treatments to break dormancy of potato minitubers of Macaca, with short dormancy, and SMIJ461-1, with long dormancy. The

  9. 钾高效基因型马铃薯钾素利用效率机制研究%K + Utilization Mechanism Study of High K+-Efficient Materials Potato (Solanum tuberosum L.)

    Institute of Scientific and Technical Information of China (English)

    黄雪丽; 胡应锋; 邹雪; 王余明; 王西瑶

    2013-01-01

    Two potassium efficient genotypes (HEK-1,HEK-2) of potato (Solanum tuberosum L.) were used to study the potassium utilization mechanism by determination of yield of fresh tuber,biomass and potassium uptake in different parts of the plant,kinds and concentrations of organic acids in root exudes,and the potassium release ability of root exudes under hydroponic and sand cultivation conditions.The results showed that compared to CK,in the low potassium concentration,HEK-1 could keep higher yield of dry mater of root; at every growth period higher potassium content was maintained in the stalk and at the maturation period,the plant potassium utilization efficiency was the highest of all ; HEK-2 at low potassium level,the content of ethanedioic acid in organics what was secreted by the root was higher than that of HEK-1 and CK,and it can activate potassium in surrounding soil better than other two materials.%为探索马铃薯钾高效机制,利用营养液沙基盆栽方法,对比研究了不同钾处理下2个钾高效材料(‘HEK-1’和‘HEK-2’)和1个普通品种(‘合作88,)的单株鲜薯产量、植株各部位在不同生长时期的干物质和钾素积累量、根系分泌有机酸种类与含量以及根系分泌物对土壤中钾的活化能力等评价作物钾效率的相关指标.结果表明,与普通品种相比,低钾浓度下,‘HEK-1’保持较高的根系干物重,各生长时期地上茎中均保持较高的钾含量,成熟期植株钾利用效率高;‘HEK-2’低钾胁迫下根系分泌物中草酸含量较多,其根系分泌物活化土壤中钾的能力强.结论:不同基因型的钾高效材料耐低钾机制不同,提高植株钾利用效率或增加根系分泌物中的草酸含量以促进钾吸收都属于马铃薯应答低钾胁迫的有效方式.

  10. Treated Wastewater Reuse on Potato (Solanum Tuberosum)

    DEFF Research Database (Denmark)

    Battilani, A; Plauborg, Finn; Andersen, Mathias Neumann;

    2014-01-01

    ) was treated by Membrane Bio Reactor (MBR) technology and gravel filter (FTS) during three cropping seasons. Treated wastewater, soil and tubers were analysed for the faecal indicator bacterium E. coli and heavy metals contents. Potato total yield was similar for tap and reused water, while the...... contents. Only for boron and zinc were differences recorded. The reused water contribution in term of nutrients value for FTS was up to 108 euro ha-1 while MBR water reduced fertiliser costs by up to 114 euro ha-1....

  11. Variedade de batatinha "Eigenheimer" (Solanum tuberosum L.

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    O. J. Boock

    1950-01-01

    Full Text Available The behavior of the Irish potato variety "Eigenheimer" in the State of São Paulo has been studied. This variety grows well under the soil and climatic conditions present in potato areas of this state. Eigenheimer is an early maturing variety and a good yielder. The tubers sprout readily in storage, permitting two plantings in a year to be made with same seed. It is susceptible to virus diseases, early and late blight, bacterial wilt, common scab, and root-knot nematode. It shows a tendency to develop second growth, and under rainy and hot weather is subject to internal brown spot. The tubers of Eigenheimer are deep-eyed, and a large percentage of the crop consists of average and small-sized tubers. Trie marketing value of the Eigenheimer potato equals that of Konsuragis, Parana Ouro, and Voran, being higher than that of white-fleshed varieties, but lower than the value of Bintje and Eersteling.

  12. Kapitola 6. Brambory (Solanum tuberosum L.)

    OpenAIRE

    Dvorak, Petr; Tomasek, Jaroslav; Hamouz, Karel

    2014-01-01

    In the area of potato production, targeted research solving concrete and actual problems of potato producers runs on Czech University of Life Sciences in Prague. In the last few years, we were focused on the production of new potatoes designated for early harvest, and we were focused on capitalization of yielding and qualitative characteristics of colored potato variety. These findings were further utilized and transferred to the system of organic farming. Firstly, we watched the influence of...

  13. Simulação do desenvolvimento da batata cultivar asterix em cinco cenários de mudanças climáticas em Santa Maria, RS Simulation of potato cultivar asterix development under five climate change scenarios in Santa Maria

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    Nereu Augusto Streck

    2006-01-01

    Full Text Available Em estudos do impacto de mudanças climáticas sobre agroecossistemas, um aspecto importante a quantificar é o efeito do aquecimento global sobre o desenvolvimento vegetal. O objetivo desse trabalho foi simular o impacto de cenários climáticos de aumento de temperatura do ar no desenvolvimento da batata (Solanum tuberosum L., em Santa Maria, (RS. Cenários meteorológicos de mudanças climáticas (+2 °C, +3 °C, +4 °C, +5 °C e +6 °C para os próximos cem anos foram gerados com Weather Generator LARS-WG usando-se como base a série de dados meteorológicos observados de 1969 a 2003, em Santa Maria, RS. Por meio de um modelo matemático, calculou-se a taxa diária de desenvolvimento da planta e a data dos principais estágios de desenvolvimento da batata, cv. Asterix: emergência, início de tuberização, início de senescência e colheita. Foram consideradas duas épocas de cultivo da batata, o cultivo de outono (plantio em 10/2 e o cultivo de primavera (plantio em 20/7. Verificou-se, para o cultivo de outono, um grande aumento (até 42 dias no ciclo total (emergência-colheita com o aumento da temperatura do ar. Já para o cultivo de primavera, obteve-se uma tendência de um pequeno encurtamento (5-8 dias do ciclo total da batata com o aumento da temperatura média do ar, dependendo do nível de aquecimento. Fases intermediárias do desenvolvimento da batata poderão ter alterações que dependem da época de cultivo. Essas diferentes tendências no comportamento do desenvolvimento da batata refletem a complexidade da resposta dos agroecossistemas à mudança climática.In studies of the impact of climate change on agroecosystems, it is important to quantify the effect of global warming on crop development. The objective of this study was to simulate the impact of increasing air temperature scenarios on the potato (Solanum tuberosum L. development in Santa Maria, Rio Grande do Sul State. Climate change scenarios of increasing air

  14. Molecular evolution of the disease resistance gene Rx in Solanum

    OpenAIRE

    Butterbach, P.B.E.

    2007-01-01

    Potato (Solanum tuberosum ssp. tuberosum) is the fourth most important food crop with an annual yield of about 300 million tons over the world. The history of the domestication of potato shows that disease-causing agents followed the tracks of potato cultivation in temperate climates across continents, resulting in substantial crop losses. Plants including potato have evolved defence mechanisms against pathogens, of which the pathotype-specific system involving resistance genes (R genes) is v...

  15. Seleção precoce de clones de batata para caracteres do tubérculo Early selection of potato clones for tuber characters

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    Geovani Bernardo Amaro

    2003-06-01

    Full Text Available Nos últimos anos, foi despertado um grande interesse para o processamento da batata na forma de fritura no Brasil. Visando à obtenção de cultivares nacionais que atendam aos padrões de qualidade culinária exigidos pelas indústrias de processamento, os programas de melhoramento de batata têm estabelecido estratégias para a redução de custos e de tempo. Realizou-se este trabalho com o objetivo de avaliar a eficiência da seleção precoce de clones de batata (Solanum tuberosum L. para características de importância ao processamento na forma de fritura. Os caracteres avaliados foram: peso específico de tubérculos, índices de formato de tubérculos e notas na glico-fita, mediante correlações entre as gerações seedling (S, primeira geração clonal (C-1 e segunda geração clonal (C-2, entre clones e entre famílias. Os experimentos foram conduzidos no sul do estado de Minas Gerais, Brasil. As correlações foram significativas na maioria dos casos, porém, baixas e geralmente apresentaram maiores valores quando consideradas entre famílias, indicando a baixa eficiência da seleção precoce entre clones. Assim, deve ser praticada somente para se eliminar as piores famílias. A correlação para o índice de formato de tubérculo (comprimento/diâmetro maior entre as gerações S e C-1 foi de 0,51 entre clones e 0,77 entre famílias e ambos os valores foram significativos, indicando uma razoável eficiência da seleção precoce para esse caráter tanto entre clones como entre famílias.The purpose of this research was to assess the effectiveness of early selection for tuber specific gravity, tuber shape index, and reducing sugars content in the tubers of potato (Solanum tuberosum L. clones. The experiments were conducted in the south of the State of Minas Gerais, Brazil. Correlation coefficients were determined for the seedling (S and first (FCG and second (SCG clonal generations at the clones and families levels. In general

  16. Ação do acibenzolar-S-metil aplicado em tubérculos e plantas de batata contra canela preta, incitada por Pectobacterium carotovorum subsp. atrosepticum atípica

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    Benelli Adilce I. H.

    2004-01-01

    Full Text Available A resistência sistêmica adquirida (SAR = systemic acquired resistance é um importante mecanismo de resistência a doenças em plantas. Neste estudo, a ação do acibenzolar-S-metil (ASM, derivado benzotidiazólico ativador de resistência em plantas foi avaliada sobre a brotação de tubérculos de batata (Solanum tuberosum e quanto à ação deste na indução de resistência à canela-preta, incitada por Pectobacterium carotovorum subsp. atrosepticum atípica (Pcaa, nas cultivares Asterix, Baronesa e Monalisa. Nas doses, 60, 120, 150, 200 e 250 mg i.a. l -1, o produto não inibiu o número de brotos. Contudo, em concentrações mais elevadas influenciou o comprimento destes. Em casa de vegetação, nas concentrações de 60 e 120 mg i.a. l -1 ASM, tanto no tratamento de tubérculos quanto no de aspersão nas plantas, a cultivar Asterix, respondeu ao tratamento do ASM, conferindo-lhe resistência à canela preta. Na cultivar Baronesa, a resposta ao ASM ocorreu somente no tratamento de tubérculos, e, para a cultivar Monalisa, não houve resposta ao ASM. Verifica-se, neste estudo, que houve ação do ASM sobre a indução de resistência e que este foi específico para determinadas cultivares de batata.

  17. Evaluación de bacilos aerobios formadores de endosporas (bafes) para el control biológico de Rhizoctonia solani Kuhn en el cultivo de papa criolla (solanum tuberosum Grupo Phureja)

    OpenAIRE

    Blanco Zapata, Diana Carolina

    2012-01-01

    Rhizoctonia solani es un hongo fitopatógeno que produce la enfermedad de la Rizoctoniasis en cultivos de papa (Solanun tuberosum) a nivel mundial causando reducción en el rendimiento y la producción entre el 10% y el 26% Los mecanismos de control que se utilizan en la actualidad no permiten un control efectivo de la enfermedad, demandan altos costos y generan problemas medioambientales que estimulan a la búsqueda de estrategias alternativas y/o adicionales como el uso de bacilos formadores de...

  18. [Polymorphism of KPI-A genes from plants of the subgenus Potatoe (sect. Petota, Estolonifera and Lycopersicum) and subgenus Solanum].

    Science.gov (United States)

    Krinitsyna, A A; Mel'nikova, N V; Belenikin, M S; Poltronieri, P; Santino, A; Kudriavtseva, A V; Savilova, A M; Speranskaia, A S

    2013-01-01

    Kunitz-type proteinase inhibitor proteins of group A (KPI-A) are involved in the protection of potato plants from pathogens and pests. Although sequences of large number of the KPI-A genes from different species of cultivated potato (Solanum tuberosum subsp. tuberosum) and a few genes from tomato (Solanum lycopersicum) are known to date, information about the allelic diversity of these genes in other species of the genus Solanum is lacking. In our work, the consensus sequences of the KPI-A genes were established in two species of subgenus Potatoe sect. Petota (Solanum tuberosum subsp. andigenum--5 genes and Solanum stoloniferum--2 genes) and in the subgenus Solanum (Solanum nigrum--5 genes) by amplification, cloning, sequencing and subsequent analysis. The determined sequences of KPI-A genes were 97-100% identical to known sequences of the cultivated potato of sect. Petota (cultivated potato Solanum tuberosum subsp. tuberosum) and sect. Etuberosum (S. palustre). The interspecific variability of these genes did not exceed the intraspecific variability for all studied species except Solanum lycopersicum. The distribution of highly variable and conserved sequences in the mature protein-encoding regions was uniform for all investigated KPI-A genes. However, our attempts to amplify the homologous genes using the same primers and the genomes of Solanum dulcamarum, Solanum lycopersicum and Mandragora officinarum resulted in no product formation. Phylogenetic analysis of KPI-A diversity showed that the sequences of the S. lycopersicum form independent cluster, whereas KPI-A of S. nigrum and species of sect. Etuberosum and sect. Petota are closely related and do not form species-specific subclasters. Although Solanum nigrum is resistant to all known races of economically one of the most important diseases of solanaceous plants oomycete Phytophthora infestans aminoacid sequences encoding by KPI-A genes from its genome have nearly or absolutely no differences to the same from

  19. Reação de cultivares de batata à podridão mole causada por Pectobacterium carotovorum subsp. atrosepticum, por P. carotovorum subsp. carotovorum e por P. chrysanthemi Reaction of potato cultivars to soft rot caused by Pectobacterium carotovorum subsp. atrosepticum, P. carotovorum subsp. carotovorum and P. chrysanthemi

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    Adilce I. H. Benelli

    2004-04-01

    Full Text Available A podridão mole em tubérculos de batata (Solanum tuberosum, causada por Pectobacterium carotovorum subsp. atrosepticum, por Pectobacterium carotovorum subsp. carotovorum e por P. chrysanthemi, é uma preocupante doença que causa danos expressivos à cultura em todo o mundo. Como inexiste tratamento eficiente para a podridão mole, o desenvolvimento de cultivares resistentes é considerado o método mais eficaz para a redução de perdas causadas pela doença. Nesse sentido, quatro cultivares de batata foram avaliados quanto à resistência natural às pectobactérias, mediante redução de massa de tubérculos após 20, 24, 48, 72 e 96 h de inoculação com suspensões bacterianas. O delineamento experimental constou de um esquema fatorial com quatro cultivares, três bactérias e quatro repetições. Os resultados foram transformados em proporção e integralizados como área abaixo da curva de progresso da doença (AACPD. Para as três bactérias estudadas, a cultivar Asterix mostrou-se o menos suscetível à podridão mole, diferindo significativamente dos demais.Soft rot caused by Pectobacterium carotovorum subsp. atrosepticum, Pectobacterium carotovorum subsp. carotovorum and P. chrysanthemi is a severe disease of potato (Solanum tuberosum worldwide. Since there is no efficient treatment for soft rot, the use of resistant cultivars is considered the most effective method to reduce crop losses due to this disease. In this research, four potato cultivars were evaluated regarding their resistance to pectobacteria. Tubers inoculated with bacterial suspensions were assessed for mass reduction at five periods (20, 24, 48, 72, and 96 h after inoculation. The experimental design consisted of a factorial scheme with four cultivars, three bacteria, and four replications. The data on tuber mass loss were transformed into proportion and integrated as the area under the disease progress curve. For all three bacteria, the cultivar Asterix was the less

  20. Ocorrência de pectobactérias em tubérculos de batata-semente no estado do Rio Grande do Sul Ocurrence of pectobacteria in potato seed tubers in Rio Grande do Sul

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    Samira O. M. El Tassa

    2004-12-01

    Full Text Available Tubérculos de batata (Solanum tuberosum-semente, pré-básica, básica, registrada e certificada, de oito cultivares, oriundos de 21 lavouras localizadas nos municípios de Vacaria, Canguçu, Piratini e Ibiraiaras, no Rio Grande do Sul, foram coletados nos meses de maio a agosto de 2002. Cada tubérculo foi lavado em água corrente, deixado secar à temperatura ambiente, perfurado com palitos em dez lenticelas, coberto com fina camada de óleo de soja, colocado individualmente em cima de folha de papel toalha umedecida dentro de saco plástico transparente e incubado a 23 ºC por quatro dias. A incidência de podridão mole a partir das lenticelas variou de 20_100% entre as cultivares. Pectobacterium sp. foi constatada em tubérculos das 21 lavouras. Duzentos e vinte e três isolados de Pectobacterium sp. foram obtidos em meio CPG, a partir das lenticelas com podridão mole, e identificados por testes bioquímicos, fisiológicos e PCR em nível de subespécie. Cento e dezenove isolados foram identificados como P. carotovorum subsp. brasiliensis e e 96 com o P. carotovorum subsp. carotovorum. Oito isolados não se enquadraram na classificação bioquímica. Pectobacterium carotovorum subspp. estavam presentes em tubérculos de batata-semente, independente da cultivar, classe ou município de origem. Pectobacterium carotovorum subsp. atrosepticum, a principal responsável por causar canela preta em batata em outros países, não foi detectada.Seed potato (Solanum tuberosum tubers from eight cultivars of 21 fields in Vacaria, Canguçu, Piratini and Ibiraiaras, all in the state of Rio Grande do Sul, were harvested from May to August of 2002. Each tuber was washed in running water, left to dry at environment temperature (23 ºC, stabbed with toothpicks in ten lenticels, covered with a thin layer of soybean oil, put individually on a wet paper towel inside a transparent plastic bag and incubated at 23 ºC for four days. The incidence of soft rot in

  1. Stock indexing and Potato virus Y elimination from potato plants cultivated in vitro Indexação de matrizes e eliminação do Potato virus Y em plantas de batata cultivadas in vitro

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    Luciana Cordeiro Nascimento

    2003-01-01

    Full Text Available Potato cultivars (Solanum tuberosum L. have shown degeneration or run out caused by viruses after several cycles of propagation using seed tubers from commercial fields. This work reports the occurrence of single and mixed infections of four potato viruses in Paraíba-Brazil and presents a method for Potato virus Y (PVY elimination, by using thermo-and chemotherapies. Plants of potato cv. Baraka were tested by direct antigen coating ELISA. Antisera against PVY, Potato virus X (PVX, Potato virus S (PVS, and Potato leafroll virus (PLRV were used. Materials with positive reaction to PVY were treated for virus elimination. Single node cuttings (1.0 cm length were excised and inoculated in Murashige & Skoog (MS medium, supplemented with 1.0 mg L-1 of kinetin, 0.001 mg L-1 of naphthalene acetic acid (NAA and 0.1 mg L-1 of gibberellic acid (GA3. The thermotherapy at approximately 37ºC, during 30 and 40 days, resulted in 20.0 and 37.5% PVY elimination, respectively. Chemotherapy was undertaken with Ribavirin (RBV, 5-Azacytidine (AZA, and 3-Deazauridine (DZD. The RBV showed the highest rate of virus eradication, with 55.5% virus-free plants. Simultaneous thermo and chemotherapy had higher efficiency for the elimination of PVY, reaching rates of healthy plants of 83.3% with RBV, 70.0% with AZA, and 50.0% with DZD.Cultivares de batata (Solanum tuberosum L. têm mostrado degenerescência causada por vírus após ciclos sucessivos do uso de tubérculos de campos comerciais como material propagativo. Este trabalho verifica a ocorrência de infecção simples e mista de quatro vírus da batata na Paraíba e apresenta adequação da técnica de cultivo in vitro para obtenção de material livre de Potato virus Y (PVY, incluindo uso de microestacas, termo e quimioterapia. Plantas de batata do cv. Baraka foram submetidas à indexação sorológica pelo teste "direct antigen coating" ELISA. Utilizaram-se antissoros contra o PVY, Potato virus X (PVX, Potato virus

  2. Produtividade e principais problemas fitossanitários de cultivares de batata em Sergipe Yield and phytosanitary problems of potato cultivars in Sergipe, Brazil

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    Maria Urbana Corrêa Nunes

    2002-09-01

    Full Text Available Sete cultivares de batata (Solanum tuberosum L. foram avaliadas durante três anos em Itabaiana-SE, com o objetivo de verificar a potencialidade de produção à altitude de 180 m e identificar os problemas fitossanitários que afetam a produtividade. Os experimentos foram conduzidos nos meses de junho a setembro, utilizando-se o delineamento experimental de blocos ao acaso, com cinco repetições. Após a colheita foram avaliadas a produção total, comercial (tubérculos sadios e com peso mínimo de 40 g e perdas de produção decorrentes de sarna, podridão-mole, nematóide e insetos do solo. A análise conjunta das produções totais e comerciais de tubérculos revelou que as cultivares mais produtivas foram Baronesa (33,7 t/ha, Monalisa (32,7 t/ha e Elvira (31,4 t/ha. A produção comercial variou de 3,4 t/ha a 22,5 t/ha, destacando-se as cvs. Elvira e Monalisa, com rendimentos de 22,5 t/ha e 20,5 t/ha respectivamente, com apenas três pulverizações com defensivos. Os problemas fitossanitários que mais afetaram a cv. Elvira foram sarna (13,1% e nematóide das galhas (7,2%, enquanto a 'Monalisa' teve sua produção prejudicada mais pela incidência de insetos (15,1%, sarna (12,0% e nematóide (9,7%. A cv. Monalisa apresentou tubérculos com melhores características para comercialização e está sendo cultivada a nível comercial em substituição às variedades Bintje e Baraka anteriormente cultivadas na região. Os resultados indicam a potencialidade da região de Itabaiana para a produção de batata.The yield potential and main diseases affecting productivity of seven potato (Solanum tuberosum L. cvs. were evaluated during a three years period in experiments carried out at low altitude conditions of Sergipe State, Brazil. The experimental plots were located at 180 m of sea level in a Red-Yellow Podzolic sandy-clay texture soil. The experimental design was of complete randomized blocks with five replications. Tubers were sown yearly

  3. Inibição da brotação de tubérculos de batatinha - Solanum Tuberosum L. Treating irish potato tubers with sprout inhibiting products

    Directory of Open Access Journals (Sweden)

    0. J. Boock

    1959-01-01

    Full Text Available Em prosseguimento aos trabalhos iniciados em 1946, referentes ao uso de substâncias inibidoras da brotação de tubéreulos de batatinha usadas tanto para o consumo como para o plantio, são relatados agora os estudos mais recentes e nos quais foram obtidos resultados dos mais animadores, sobrepujando aquêles já publicados (1. Assim, ficou evidenciado que o produto comercial "Ipnogerm", à base de naftil-acetato de metilo, foi um dos mais eficientes na redução da brotação de tubéreulos de batatinha e na perda de pêso, seguido de "Aaservo", à base de isopropil-fenil-carbamato, enquanto o "Barsprout", à base do éster metflico do ácido alfa-naftaleno acético, foi o que mais impediu o aparecimento de podridão mole, ao contrário do "Aaservo". Em relação à podridão sêca, causada por Fusarium, os produtos mais recomendados foram "Fusarex", à base de tetra-cloro-nitro-benzeno, "Agermina", à base de éster metílico do ácido alfa-naftaleno-acético, e "Ralentone", à base de alcani-metil-naftaleno. De um modo geral, os produtos "Ipnogerm", "Aaservo" e "Tuberite", êste último à base de isopropil-fenil-carbamato, não devem ser empregados para batatas-semente, ao contrário do "Fusarex", "Ralentone" e ''Agermina", fato êste comprovado pelo plantio dos tubérculos tratados.Several sprout inhibiting products were tested on Irish potato tubers. "Ipnogerm", a methyl-acetate compound, gave best results, reducing tuber sprouting and loss of weight, "Aaservo", on the basis of isopropil-phenyl-earbamate, was less effective, while "Barsprout", a methyl ester of the alfa-naphtalene acetic acid, was the most effective on the control of tuber soft rot. The dry rot disease caused by Fusarium, during storage, was better controlled by "Fusarex", a tetra-chloro-nitro-bcnzene product, ''Agermina", a methyl-ester of the alfa-naphtalene acetic acid, and "Ralentone", on the basis of alkani-methyl-naphta-lene. As a rule, "Ipnogerm", "Aaservo", and

  4. Taxonomic Treatment of Solanum Section Petota (Wild Potatoes) in Catálogo de Plantas Vasculares del Cono Sur (Argentina, Chile, Paraguay, Uruguay, y sur del Brasil)

    Science.gov (United States)

    Solanum section Petota (Solanaceae), which includes the cultivated potato (Solanum tuberosum) and its wild relatives, contains over 150 wild species distributed from the southwestern U.S.A. (38°N) to central Argentina and adjacent Chile (41°S). This catalog includes all species from the Southern Con...

  5. Polyphenols from Eriosema tuberosum.

    Science.gov (United States)

    Ma, W G; Fuzzati, N; Li, Q S; Yang, C R; Stoeckli-Evans, H; Hostettmann, K

    1995-07-01

    A dichloromethane extract of the roots of Eriosema tuberosum exhibited antifungal activity against Cladosporium cucumerinum and Candida albicans using TLC bioautography. Bioassay-directed fractionation led to the isolation of four new compounds, eriosemaones A-D, together with a known compound, flemichin-D, as the active constituents. Three inactive polyphenols were also isolated after methylation, together with one new chromone, eriosematin. Structures were determined by spectroscopic analysis and from chemical evidence. PMID:7662271

  6. Evaluación preliminar del efecto que sobre algunas enzimas del suelo tienen diferentes mecanismos de control del hongo (Phytophthora infestans en un cultivo de papa (Solanum tuberosum bajo dos tipos de manejo agrícola (orgánico y convencional

    Directory of Open Access Journals (Sweden)

    Guardiola Marta Lucía

    2004-12-01

    Full Text Available Se evaluó comparativamente el posible efecto diferencial que, sobre la actividad de las enzimas proteasa, fosfatasa ácida, fosfatasa alcalina y celulasa, además del pH y la materia orgánica del suelo, tuvieron los métodos de control de Phytophthora infestans tanto en la agricultura orgánica (purín de Chipaca Bidens pilosum como en la convencional (diversos fungicidas comerciales en plantas de papa (Solanum tuberosum. Igualmente, en los parámetros antes mencionados, se evaluó el efecto de un promotor fúngico utilizado como control del mismo hongo en ambas agriculturas. La investigación se dividió en una fase de campo y una de laboratorio. En la fase de campo, realizada durante siete meses de 1999, se acompañó la siembra, el desarrollo del cultivo y evolución de la enfermedad a través de la determinación de la incidencia y su severidad. En el laboratorio se determinó la actividad enzimática proteasa, fosfatasa ácida, fosfatasa alcalina, celulasa, así como también el pH y porcentaje de materia orgánica. Se encontró que en el manejo agrícola orgánico la actividad de la fosfatasa ácida, la fosfatasa alcalina y la proteasa así como también el pH fueron más influenciados por el estado de desarrollo de las plantas que por efecto del los tratamientos utilizados en el control del hongo. En el manejo agrícola convencional la actividad enzimática de las celulasas fue inhibida significativamente por los fungicidas. La actividad de las proteasas y fosfatasas ácidas fueron estimuladas por efecto de los fungicidas comerciales y por el estado de desarrollo de las plantas. Las actividades enzimáticas fosfatasa ácida, proteasa y celulasa presentaron comportamientos significativamente diferentes entre los cultivos convencional y orgánico. Para el control de la enfermedad, el uso de los fungicidas fue el tratamiento que mejores resultados presentó con una mayor producción de papa pero con mayores gastos de producción, mientras

  7. EXPRESIÓN DE LA PROTEÍNA Cry1Ac EN TEJIDOS DE LÍNEASTRANSGÉNICAS DE PAPA (Solanum tuberosum spp. ANDÍGENAVAR. DIACOL CAPIRO. Cry1Ac Protein Expression in Tissues of Potato (Solanumtuberosum spp. Andígena Transgenic Lines Var. Diacol Capiro

    Directory of Open Access Journals (Sweden)

    PABLO ANDRÉS VANEGAS ARAUJO

    genetic plant breeding related to the introduction of Cry genes which codify insecticidal crystal proteins is an alternative for reducing the insect attack in commercial crops. In this work, the insertion, transcription and expression of Cry1Ac gen was characterized in different tissues and three development stages of two transgenic lines of Solanum tuberosum variety Diacol Capiro that were previously transformed by Agrobacterium tumefaciens method. The characterization was realized by PCR, RT-PCR and ELISA techniques. The gen insertion and transcription was confirmed using primers for Cry1Ac gen that amplified a specific band of 766 bp. The protein expression levels were higher than 45 µg/g and were not significantly different between the analyzed lines nor the three development stages. Furthermore, taking into account some relevant phenotypic features, no significant differences were found between transgenic lines and controls. The results suggest that monitoring and biosecurity assays are necessary with this vegetal material because their high level expression inside all the tissues analyzed that could affect non-targeted insects.

  8. Metodologia multicritério de apoio à decisão para avaliar sistemas de produção de batata orgânica na região Sul do Rio Grande do Sul.

    OpenAIRE

    Reichert, Lírio José; Gomes, Mário Conill; Schwengeber, José Ernani

    2013-01-01

    A crescente demanda por batata (Solanun tuberosum L.) e outras hortaliças cultivadas organicamente, representa uma importante oportunidade para alcançar mercados diferenciados e, ao mesmo tempo um desafio para os produtores. Os agricultores, ainda carecem de informações tecnológicas e mercadológicas para a obtenção de índices técnicos e econômicos satisfatórios. Por estes e outros motivos, em geral, a produção de batata no sistema orgânico é menor em comparação com a obtida no sistema convenc...

  9. Adubos fosfatados na cultura da batata

    Directory of Open Access Journals (Sweden)

    Olavo José Boock

    1945-05-01

    Full Text Available 1 - As experiências relatadas no presente trabalho se referem ao emprêgo de diferentes adubos fosfatados na cultura da batata (S. tuberosum L. e conduzidas em Campinas, Taubaté e Joanópolis, em 1940 e 1944. 2 - As análises sumárias das terras indicaram variações do fósforo, desde muito fraco (Joanópolis, regular (Campinas até ótimo (Taubaté . 3 - As menores produções foram obtidas dos canteiros sem adubo. 4 - O emprêgo ùnicamente de adubos azotados mais potássicos, pràticamente, não determinou aumento de produção sôbre os "sem adubo". 5 - O acréscimo de adubos fosfatados aos azotados e potássicos causou um aumento altamente significativo de produção. 6 - Os canteiros em que além dos adubos azotados e potássicos, incluímos o Superfosfato apresentaram em todos os ensaios as melhores colheitas. 7 - Quanto aos demais adubos, pequenas foram as diferenças existentes.The experiments herein reported deal mainly with the comparative effect of five sources of phosphate on the yield of the potato plant. The trials were carried out at three localities in the State of São Paulo, viz. : Campinas, Taubaté and Joanópolis. Soil analyses from the test plots showed variation in phosphorus content. This was low at Joanópolis, medium at Campinas, and high at Taubaté. Nitrogen was added as ammonium sulphate, nitrate of soda, and potassium nitrate ; potash, as potassium sulphate or potassium carbonate (ashes. Phosphorus was suplied by superphosphates (trade names of Dawco and Granulated, of American origin by "Cibraphosphate", "Serranaphosphate" and "Serranaphosphatepotassico" from local manufacturers and by bone meal. All forms of nitrogen or potash tried were about equally effective for potatoes when applied with any of the phosphates. Phosphorus was found to be the most important element to increase production. Applications of only nitrogen plus potash, brought no significant increase in yield. The greatest yields were obtained

  10. Diversity and evolution of resistance genes in tuber-bearing Solanum species

    NARCIS (Netherlands)

    Wang, M.

    2007-01-01

    Potato (Solanum tuberosum L.) is a crop with a large secondary gene pool, which contains many important traits that can be exploited in breeding programs. As late blight is one of the biggest problems in potato growing areas, the crop needs a large number of applications of fungicides to be able to

  11. Early selection of full-sib potato families Seleção precoce de famílias de irmãos-completos de batata

    Directory of Open Access Journals (Sweden)

    Dheyne Silva Melo

    2011-12-01

    Full Text Available Clonal selection is the preferred breeding method used in potatoes (Solanum tuberosum L.. However this selection procedure is only efficient for more advanced generations and shows no good results when applied in the seedling up to the second clonal generation. This study assessed the feasibility of selection in early generations of full-sib potato families and compares the selection method among and within families with the combined selection under different selection intensities. Six experiments were conducted from the first (C1 until the third clonal generation (C3. In C1 a randomized complete block design with four replications of 25 plants was used. In the remaining generations RCB was employed with three replications of 10 plants. Genetic variances were lower between families than within families, for all traits, but the heritabilities between families were almost always larger. The expected gains from selection between and within families were superior to gains from the combined selection in any intensity of selection. The selection of families should have weaker intensity than selection among clones within families. The selection of families was more efficient when based on the average of environments.A seleção clonal é o método de melhoramento preferencialmente usado no melhoramento da batata (Solanum tuberosum L.. Contudo, esse método de seleção somente é eficiente em gerações avançadas e não apresenta bons resultados quando aplicado da geração seedling até a segunda geração clonal. Objetivou-se, neste trabalho, verificar a viabilidade da seleção de famílias em gerações precoces e comparar o método de seleção entre e dentro de famílias com a seleção combinada, sob diferentes intensidades de seleção. Seis experimentos foram conduzidos desde a primeira (PGC até a terceira geração clonal. Na PGC, o delineamento foi DBC com quatro repetições e parcelas de 25 plantas. Nas demais gerações, empregaram

  12. MARUCA: UNA NUEVA VARIEDAD CUBANA DE PAPA (Solanum tuberosum L.

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    Ana Estévez

    2007-01-01

    Full Text Available En el Instituto Nacional de Ciencias Agrícolas (INCA, desde 1985 se inició un programa de mejoramiento genético, con el objetivo de obtener variedades cubanas de papa con resistencia a enfermedades, altos rendimientos y calidad de los tubérculos; producto de ese programa se han obtenido seis variedades. Maruca es una nueva variedad obtenida por el método clásico de hibridación, que posee altos rendimientos, calidad para consumo fresco y es medianamente susceptible a A. solani. Posee su registro comercial de nueva variedad cubana, otorgado por el Centro Nacional de Sanidad Vegetal del Ministerio de Agricultura de Cuba. En este trabajo se presentan las principales características de esta variedad así como su pedigrí.

  13. Somatic hybridization between Lycopersicon esculentum and Solanum tuberosum.

    NARCIS (Netherlands)

    Schoenmakers, H.C.H.

    1993-01-01

    This thesis describes an analysis of the possibilities and limitations of asymmetric somatic hybridization between tomato and potato. For this, nitrate reductase-deficient tomato mutants were isolated and characterized. It was shown that two of these mutations could be used as selectable markers in

  14. Potato (Solanum tuberosum) response to simulated glyphosate drift

    Science.gov (United States)

    Field studies were conducted in 2008 in Ontario, OR and Paterson, WA to determine the effect of simulated glyphosate drift on 'Ranger Russet' potato injury, shikimic acid accumulation, and tuber yield. Glyphosate was applied at 8.5-, 54-, 107-, 215-, and 423 g ae ha-1; which corresponds to 0.01, 0.0...

  15. Extensive Variation in Fried Chip Color and Tuber Composition in Cold-Stored Tubers of Wild Potato (Solanum) Germplasm

    Science.gov (United States)

    Cold-induced sweetening and browning in the Maillard reaction have driven extensive research in the areas of plant physiology, biochemistry, and food science in Solanum tuberosum. To date, research in these areas excluded wild relatives of potato. This is the first assessment of cold-stored tuber c...

  16. Freqüência de biovares de ralstonia solanacearum em diferentes cultivares e épocas de cultivo de batata no rio grande do sul Frequency of biovars of Ralstonia solanacearum on different cultivars and potato planting seasons in Rio Grande do Sul

    Directory of Open Access Journals (Sweden)

    JOÃO L.N. MACIEL

    2001-12-01

    Full Text Available A presença das biovares I e/ou II de Ralstonia solanacearum em uma lavoura de batatas (Solanum tuberosum tem influência direta no sucesso das medidas adotadas para controlar a murcha bacteriana. As biovares diferem entre si em relação à agressividade, latência e sobrevivência. Assim, um experimento de campo foi conduzido em uma área naturalmente infestada em duas épocas de cultivo com os objetivos de verificar (1 a incidência de biovares I e/ou II, (2 a relação entre biovar e época de plantio e (3 a relação entre biovar e cultivar de batata. Os isolados obtidos de plantas das cultivares Achat, Baronesa, Elvira, Macaca, Monte Bonito e Trapeira foram identificados como biovar I ou II através da PCR, utilizando os oligonucleotídeos iniciadores T3A e T5A. Ambas as biovares foram encontradas na área naturalmente infestada. De 73 isolados de R. solanacearum, 94,5% foram identificados como biovar II e 5,5% como biovar I. A biovar II foi isolada dos cultivos de primavera e de outono, independente da cultivar, mas a I apenas do cultivo de primavera e de plantas assintomáticas das cultivares Achat e Macaca. A maior população da biovar I nestas duas cultivares pode ser uma evidência da possível relação entre biovar e cultivar.The occurrence of biovars I and/or II of Ralstonia solanacearum in a potato (Solanum tuberosum field has direct consequences for the success of the measures adopted to control bacterial wilt. Biovars differ regarding aggressiveness, latency and survival. An experiment was conducted in a naturally infested potato field in two seasons to find (1 the incidence of biovars I and II, (2 the relationship between biovar and planting season, and (3 the relationship between biovar and potato cultivar. Bacterial isolates from potato cultivars Achat, Baronesa, Elvira, Macaca, Monte Bonito, and Trapeira were identified as biovar I or II through PCR, using T3A and T5A primers. Both biovars I and II were found in the

  17. Novel inter-series hybrids in Solanum, section Petota.

    Science.gov (United States)

    Dinu, I I; Hayes, R J; Kynast, R G; Phillips, R L; Thill, C A

    2005-02-01

    Sexual hybrids between distantly related Solanum species can undergo endosperm failure, a post-zygotic barrier in inter-species hybridizations. This barrier is explained by the endosperm balance number (EBN) hypothesis, which states that parents must have corresponding EBNs for viable seed formation. Tests for inter-crossability were made involving the Mexican species Solanum pinnatisectum Dunal. (series Pinnatisecta, ApiApi, 1EBN), autotetraploids of this species, Solanum verrucosum Schlechtd. (series Tuberosa, AA, 2EBN), haploids (2x, 2EBN) of the South American S. tuberosum L. (series Tuberosa, A1A1A2A2, 4EBN), and F2 haploid-species hybrids with South American species (AA, 2EBN) S. berthaultii Hawkes, S. sparsipilum (Bitter.) Juz. and Bukasov and S. chacoense Bitter. The development of hybrid endosperms was investigated for these combinations by confocal microscopy with regard to cell-division timing and tissue collapse. Novel sexual diploid (AApi) and triploid (AApiApi) inter-series hybrids were generated from S. verrucosum x S. pinnatisectum crosses by using post-pollination applications of auxin. F1 embryos were rescued in vitro. The hybrid status of recovered plants was verified by microsatellite marker analysis, and the ploidy was determined by chromosome counting. The application of phytohormones in inter-ploidy S. pinnatisectum x S. tuberosum crosses, however, did not delay endosperm collapse, and embryos were not formed. Other diploid, 1EBN species tested in remote hybridizations with Group Tuberosum were S. cardiophyllum Lindl., S. trifidum Correll, and S. tarnii Hawkes and Hjert., series Pinnatisecta, and S. bulbocastanum Dunal., series Bulbocastana. Based on the analysis of post-zygotic reproductive barriers among isolated species of section Petota, we propose strategies to overcome such incompatibilities. PMID:15517147

  18. Preservação in vitro da batata com ácido acetilsalicílico e duas fontes de carboidrato In vitro storage of potato under acetyl salicylic acid and two carbohydrate sources

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    Gerson Renan de Luces Fortes

    2001-10-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito de carboidratos e do ácido acetilsalicílico (AAS na preservação in vitro da batata (Solanum tuberosum L., cultivar Macaca. Brotações de 1,5 a 2,0 cm de comprimento foram transferidas para meio de MS, acrescido de mio-inositol (100 mg L-1 e ágar (6 g L-1. Testaram-se duas fontes de carboidrato, sacarose e manitol (87,6 mM, e cinco concentrações de AAS (0, 30, 60, 90 e 120 mg L-1. O delineamento foi em blocos casualizados com quatro repetições por tratamento e cada repetição formada por oito tubos de ensaio com uma brotação. O material foi mantido à temperatura de 25±2ºC, fotoperíodo de 16 horas e radiação de 19 miE m-2 s-1. O crescimento e o número de gemas nas hastes foram avaliados por três meses. Passados nove meses, a sobrevivência e o número de microtubérculos também foram avaliados. O uso de manitol, associado às concentrações a partir de 30 mg L-1 de AAS, proporcionou menor crescimento e formação de gemas nas hastes. No meio suplementado com sacarose, a sobrevivência e o número de microtubérculos foram maiores, independentemente das concentrações de AAS utilizadas, após nove meses de cultivo.The aim of this work was to evaluate the effect of carbohydrates and acetyl salicylic acid (ASA during the in vitro storage of potato (Solanum tuberosum L., cultivar Macaca. Stems derived from in vitro cultures were cut into 1.5 to 2.0 cm segments and inoculated in a MS medium supplemented with myo-inositol (100 mg L-1 and agar (6 g L-1. Sucrose and mannitol 87.6 mM and five ASA concentrations (0, 30, 60, 90 and 120 mg L-1 were tested. The stems were cultured on 10 mL medium in test tubes (20 x 150 mm and incubated in a growth chamber at 25±2ºC, 16 hour photoperiod and 19 muE m-2 s-1 radiation. The growth and the bud number formed in the stems for a period of three months were evaluated. Nine months later the survival percentage and the number of microtubers formed

  19. Late blight and early blight resistance from Solanum hougasii introgressed into Solanum tuberosum

    Science.gov (United States)

    Late blight, caused by Phytophthora infestans, and early blight, incited by Alternaria solani,are the two most widely occurring foliar diseases of potato in the U.S. Resistance to both diseases is necessary if growers are to reduce fungicide applications. Field resistance to late blight has previous...

  20. Resistance of Wild Solanum Accessions to Aphids and Other Potato Pests in Quebec Field Conditions

    OpenAIRE

    Fréchette, B.; Bejan, M.; Lucas, É.; Giordanengo, P.; Vincent, C

    2010-01-01

    Two experiments were done to determine the susceptibility of six wild potato accessions to the aphids Macrosiphum euphorbiae (Thomas) (Hemiptera: Aphididae) and Myzus persicae (Sulzer). Densities of aphid colonies were compared between caged Solanum pinnatisectum Dunal (Solanales: Solanaceae), S. polyadenium Greenmam, S. tarijense Hawkes, S. infundibuliforme Philippi, S. oplocense Hawkes, and S. stoloniferum Schlechted and Bouché, and the commercially cultivated potato plant S. tuberosum L. c...

  1. Qualidade para processamento de clones de batata cultivados durante a primavera e outono no Rio Grande do Sul Processing quality of potato clones during spring and autumn grown conditions of Rio Grande do Sul

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    Sérgio Tonetto de Freitas

    2006-02-01

    Full Text Available A industrialização da batata (Solanum tuberosum L. tem sido limitada no Brasil, quase que exclusivamente, pela falta de matéria-prima adequada. Alta qualidade do produto processado é dependente de altos teores de matéria seca, que reduz a absorção de óleo durante a fritura e confere crocância, e baixos teores de açúcares redutores, que mantêm a coloração clara das fritas. O objetivo deste trabalho foi identificar clones de batata de alta qualidade para processamento a partir da avaliação de tubérculos produzidos durante os cultivos de primavera de 2003 e outono de 2004 em Santa Maria, RS. O experimento foi conduzido em um fatorial (15 clones e duas épocas de cultivo no delineamento de blocos ao acaso, com quatro repetições. Foram avaliados os clones Dakota Rose, SMINIAIporã, SMIJ461-1, SMIJ319-1, SMIJ456-4Y, SMID040-4RY, SMIE040-6RY, SMIC148-A, SMIF165-6RY, SMIH095-1, SMINIA90244-1, SMINIA793101-3, SMINIA95043-11, Macaca e Asterix nos cultivos de primavera de 2003 e outono de 2004 em Santa Maria, RS. As condições ambientais, durante o período de produção, influenciaram a qualidade pós-colheita dos tubérculos. Os clones SMIJ461-1, SMIJ319-1, SMIJ456-4Y, SMIC148-A, SMIDO40-4RY e SMIH095-1 foram os que apresentaram o melhor desempenho nas características desejáveis para processamento, sendo superiores a Asterix, cultivada para consumo de mesa ou para processamento na forma de chips nas diferentes regiões produtoras de batata. Dentre esses clones, SMIDO40-4RY e SMIH095-1 foram os menos influenciados pelas diferenças de temperatura e insolação típicas das épocas de cultivo de outono e primavera no RS. Os clones SMIJ461-1 e SMIJ456-4Y apresentaram maior teor de matéria seca e coloração mais clara do chips no cultivo da primavera.The development of the potato (Solanum tuberosum L. processing industry has been limited in Brazil, because of the low availability of tubers with adequate quality. High dry matter

  2. MORPHOPHYSIOLOGICAL CHANGES OF IPOMOEA BATATAS (L.) LAM. DURING DROUGHT STRESS

    OpenAIRE

    La Rosa, R.; Contreras, J; Mendoza, A.; Macabilca, Y.; Gutierrez, A.

    2008-01-01

    One of the greatest problems in the world is desertification so we must to know physiological responses and morphological adaptations from species with tolerance to drought stress. Sweet potato (Ipomoea batatas Lam.) is one of the most important crops of the world, especially because of its great tolerance to drought stress. Hence, we decided to know what morphophysiological and metabolic responses has I. batatas under drought stress. So, we got cuttings of variety INA-100, fro...

  3. Wound healing activity of Ipomoea batatas tubers (sweet potato)

    OpenAIRE

    Madhav Sonkamble; Vandana Panda

    2011-01-01

    Background: Ipomoea batatas (L.) Lam. from the family Convolvulaceae is the world’s sixth largest food crop. The tubers of Ipomoea batatas commonly known as sweet potato are consumed as a vegetable globally. The tubers contain high levels of polyphenols such as anthocyanins and phenolic acids and vitamins A, B and C, which impart a potent antioxidant activity that can translate well to show wound healing effects. To check their effects on wound healing, the peels and peel bandage were tested ...

  4. Pneumonia intersticial em bovinos associada à ingestão de batata-doce (Ipomoea batatas) mofada Interstitial pneumonia in cattle fed moldy sweet potatoes (Ipomoea batatas)

    OpenAIRE

    Rafael A. Fighera; Daniela B. Rozza; José V. Piazer; Marina V. Copetti; Luiz F. Irigoyen; Claudio S.L. Barros

    2003-01-01

    Uma doença respiratória foi diagnosticada em cinco dentre 23 bovinos (21,7%) após terem sido alimentados com batata-doce (Ipomoea batatas) mofada em uma pequena propriedade rural em São Vicente do Sul, Rio Grande do Sul, Brasil. Três dos cinco bovinos afetados morreram espontaneamente, e o quarto foi sacrificado para necropsia quando mostrava sinais clínicos respiratórios avançados. A manifestação clínica iniciara cerca de 24 horas após a ingestão das batatas-doces e a evolução clínica foi de...

  5. Xanthoma tuberosum in homozygous familial hypercholesterolemia

    OpenAIRE

    Nagaraja Moorthy; Rajiv Ananthakrishna; Prabhavathi Bhat; Nanjappa, Manjunath C.

    2014-01-01

    Familial homozygous hypercholesterolemia is one of the high risk factors that can result in premature coronary arterial disease leading to severe morbidity and premature death in children and young adults. We describe a rare example of extensive xanthoma tuberosum in a case of familial homozygous hypercholesterolemia.

  6. Identification and characterisation of CYP75A31, a new flavonoid 3'5'-hydroxylase, isolated from Solanum lycopersicum

    OpenAIRE

    Bourgaud Frédéric; Larbat Romain; Slimestad Rune; Jugdé Hélène; Hehn Alain; Olsen Kristine M; Lillo Cathrine

    2010-01-01

    Abstract Background Understanding the regulation of the flavonoid pathway is important for maximising the nutritional value of crop plants and possibly enhancing their resistance towards pathogens. The flavonoid 3'5'-hydroxylase (F3'5'H) enzyme functions at an important branch point between flavonol and anthocyanin synthesis, as is evident from studies in petunia (Petunia hybrida), and potato (Solanum tuberosum). The present work involves the identification and characterisation of a F3'5'H ge...

  7. In vitro development of buds from tubers of (Solanum tuberosum L.); Desarrollo in vitro de yemas procedentes de tuberculos de patata (Solanum tuberosum L.) tratados por radiacion GAMMA

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez Gonzalez, J.; Garcia Collantes, M. A.

    1976-07-01

    The present work studies the in vitro development of buds from potato tubers subjected to gamma radiation at doses of 3, 6, 9 and 12 Krad. Ths effect of radiation was dependent on the dormant stage of the buds. Intermediate doses (6-9 Krad) did inhibit mitotic division but not cellular elongation. When irradiation is carried out at the end of the resting period, there is an apparent sprouting due to the elongation of previously formed cells. (Author) 17 refs.

  8. Taxonomy Icon Data: potato [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available potato Solanum tuberosum Solanum_tuberosum_L.png Solanum_tuberosum_NL.png Solanum_tuberos...um_S.png Solanum_tuberosum_NS.png http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Solanum+tuberosum&t...=L http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Solanum+tuberosum&t=NL http://biosciencedbc.jp/taxonomy_...icon/icon.cgi?i=Solanum+tuberosum&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Solanum+tuberosum&t=NS ...

  9. Potencialidade de processamento industrial de cultivares de batatas

    OpenAIRE

    Émerson Loli Garcia; Ezequiel Lopes do Carmo; Joaquim Gonçalves de Pádua; Magali Leonel

    2015-01-01

    A industrialização da batata tem sido limitada no Brasil, devido à falta de matéria-prima adequada, sendo a demanda suprida pelas importações de produtos processados. Oito cultivares de batata, adaptadas às condições da região serrana de Minas Gerais, safra 2011, foram avaliadas quanto a sua composição centesimal, cor e conteúdo de amilose. Os resultados obtidos mostraram que as cultivares 'Marcy' e 'Colorado' apresentam características importantes para o cozimento. As cultivares 'Marlen' e '...

  10. Solanum diploconos fruits: profile of bioactive compounds and in vitro antioxidant capacity of different parts of the fruit.

    Science.gov (United States)

    Ribeiro, Alessandra Braga; Chisté, Renan Campos; Lima, José L F C; Fernandes, Eduarda

    2016-05-18

    Solanum diploconos is an unexploited Brazilian native fruit that belongs to the same genus of important food crops, such as tomato (Solanum lycorpersicum) and potato (Solanum tuberosum). In this study, we determined, for the first time, the profile of bioactive compounds (phenolic compounds, carotenoids, ascorbic acid and tocopherols) of the freeze-dried pulp and peel of Solanum diploconos fruits, as well as of an extract obtained from the whole fruit. Additionally, the antioxidant potential of the whole fruit extract was evaluated in vitro, against reactive oxygen species (ROS) and reactive nitrogen species (RNS). Eighteen phenolic compounds were identified in the peel and pulp and 6 compounds were found in the whole fruit extract. Coumaric, ferulic and caffeic acid derivatives were revealed to be the major phenolic constituents. All-trans-β-carotene was the major carotenoid (17-38 μg g(-1), dry basis), but all-trans-lutein and 9-cis-β-carotene were also identified. The peel and pulp presented extract exhibited scavenging capacity against all tested ROS and RNS (IC50 = 14-461 μg mL(-1)) with high antioxidant efficiency against HOCl. Thus, Solanum diploconos fruits may be seen as a promising source of bioactive compounds with high antioxidant potential against the most physiologically relevant ROS and RNS. PMID:27142444

  11. Qualidade de tubérculos de batatas-semente tratados com paraquat e o desenvolvimento de uma metodologia simplificada de detecção de resíduo do herbicida Quality of seed potato tubers treated with paraquat and the development of a simplified methodology for paraquat residue detection

    Directory of Open Access Journals (Sweden)

    Welington Pereira

    1995-01-01

    Full Text Available O objetivo desse trabalho foi avaliar a qualidade de tubérculos de batatas-semente (Solanum tuberosum tratados com paraquat e desenvolver uma metodologia simplificada de detecção de resíduos de herbicida. Dois ensaios foram realizados no Laboratório da Ciência das Plantas Daninhas do Centro Nacional de Pesquisa de hortaliças, Brasília, DF. No experimento, tubérculos das cultivares Achat e Baronesa foram submersos em soluções de 0 e 200 ppm de paraquat ou injetados com 0,5 ml de soluções de 0 e 200 ppm do herbicida. O delineamento experimental foi inteiramente casualizado com 8 repetições e 12 tubérculos por parcela. Os tubérculos foram colocados em câmara fria, após a aplicação com paraquat, para quebra da dormência. Após a brotação dos tubérculos avaliou-se a qualidade interna dos mesmos, amostrando, posteriormente, 2 tubérculos de cada parcela para o plantio em vasos, sob condições de telado, para verificar possíveis danos no crescimento das plantas oriundas dos tubérculos tratados. Os tratamentos de imersão não provocaram, aparentemente, nenhum dano interno nos tubérculos, ou nem mesmo afetaram a nova geração, entretanto, os tubérculos injetados com paraquat foram severamente deteriorados e carbonizados, originando plantas bastante debilitadas. Esses resultados indicam que quando o paraquat for aplicado sob condições que favoreçam sua penetração ou translocação para o interior do tubérculo, atingindo os vasos e a polpa, pode danificá-lo severamente, prejudicar sua aparência, qualidade de produção e reduzir o desenvolvimento da nova geração de plantas oriundas dos tubérculos contaminados . No segundo experimento, desenvolveu-se uma metodologia simplificada para detectar resíduos de paraquat nos tubérculos através de colorimetria, visto que o paraquat é reduzido a um radical de cor azul na presença de ditionito de sódio (Na2S2O4 a 1% em meio básico, a qual se intensifica à medida que a

  12. Seleção assistida por marcadores para teor de matéria seca e açúcares redutores em tubérculos de batata Marker assisted selection for dry matter and reducing sugars in potato tubers

    Directory of Open Access Journals (Sweden)

    Leonardo Lopes Bhering

    2009-02-01

    Full Text Available A seleção precoce de clones que possuam níveis elevados de matéria seca e baixos teores de açúcares redutores é uma necessidade nos programas de melhoramento para a qualidade de processamento da batata (Solanum tuberosum L. na forma de palitos fritos ou chips. A seleção precoce tornou-se possível com a utilização de marcadores genéticos, visto que permitem a identificação precisa de indivíduos superiores. Assim, procura-se cada vez mais encontrar marcadores capazes de caracterizar tais indivíduos e utilizá-los via seleção assistida. O objetivo deste trabalho foi avaliar a eficiência da seleção assistida, utilizando os marcadores identificados por ANDREU (2004 que estariam associados ao teor de matéria seca e açúcares redutores em tubérculos de batata. Clones provenientes de 20 famílias foram avaliados nas gerações de plântula (P, primeira geração clonal (C1 e segunda geração clonal (C2. As estimativas das correlações simples para os caracteres entre gerações foram significativas, porém, baixas, confirmando a inviabilidade de se efetuar a seleção precoce nas primeiras gerações com base apenas em informações fenotípicas. Os marcadores utilizados forneceram um total de 16 marcas. Pela regressão múltipla stepwise, apenas sete dessas marcas tiveram associação com os caracteres estudados. Além disso, nenhuma marca associada ao teor de matéria seca de tubérculos na geração C1 teve associação significativa na geração C2. Isso também foi observado com o teor de açúcares redutores, o que é um indicativo da interação QTLs x ambientes. A seleção assistida não se mostrou eficiente em relação à fenotípica em nenhum dos casos avaliados, portanto, não sendo útil em uma possível seleção precoce. Esses resultados indicam que tais marcadores não estão próximos aos genes controladores dos caracteres desejados, sendo necessária a identificação de novos marcadores mais associados que

  13. EPIDEMIA DE PINTA-PRETA DA BATATA E COMPARAÇÃO DE MÉTODOS PARA DETERMINAR O INÍCIO DOS SINTOMAS DA DOENÇA NA CULTURA DE BATATA EM CAMPO POTATO EARLY BLIGHT EPIDEMICS AND COMPARISON OF METHODS TO DETERMINE ITS INITIAL SYMPTOMS IN A POTATO FIELD

    Directory of Open Access Journals (Sweden)

    Rodrigo Orlando Campo Arana

    2007-12-01

    Full Text Available Três experimentos foram montados entre os anos de 2000 e 2002, em condições de campo, de Viçosa, estado de Minas Gerais, com a finalidade de avaliar o efeito de epidemias de pinta-preta (Alternaria solani na produção de batata (Solanum tuberosum e determinar o inicio de sintomas da pinta preta na cultura de batata. Usando o método de gradiente de severidade da doença, foram obtidas em cada experimento quatro epidemias da doença, com diferentes severidades, desde D1 até D4, em ordem decrescente, o que permitiu comparar o efeito das epidemias no crescimento da planta e na produção de tubérculos, bem como determinar o momento de se iniciar o controle da doença. Tendo como base o tempo desde o plantio até o aparecimento dos primeiros sintomas da epidemia, foram avaliados os seguintes métodos para previsão da doença: dias acumulados (DA; graus-dia acumulados (GD, tendo como temperatura-base 7ºC; e dias fisiológicos acumulados (DF, tendo como temperatura mínima 7ºC, máxima de 30ºC e ótima de 21ºC. O estabelecimento do gradiente da doença mostrou diferenças significativas na produção em cada experimento. As maiores reduções na produção dos tubérculos ocorreram na parcela não tratada com fungicida D1, com 49,6% em 2000; 52,7% em 2001; e 58,2% em 2002. A previsão da epidemia utilizando-se os métodos DA e GD não indicou o momento certo para iniciar o controle da epidemia. O método DF foi o mais adequado, estabelecendo-se o primeiro sintoma entre 240 e 333 DF. Conclui-se que a pinta-preta, dependendo da severidade, afeta a área foliar e a produção de tubérculos. Dos métodos para determinar o estabelecimento da pinta-preta, o DF apresentou o maior potencial, recomendando-se seu uso para iniciar o manejo da doença aos 250 DF após o plantio, sempre e quando as condições ambientais favorecem o estabelecimento do patógeno.The influence of an early blight epidemic on potato tuber yield was evaluated in three

  14. Processing quality of potato tubers produced during autumn and spring and stored at different temperatures Qualidade de processamento de tubérculos de batata produzidos no outono e na primavera e armazenados em diferentes temperaturas

    Directory of Open Access Journals (Sweden)

    Sergio T de Freitas

    2012-03-01

    Full Text Available The objective of this work was to access processing quality of potato clones (Solanum tuberosum Asterix, SMINIA793101-3, and Missaukee cultivated during spring and autumn growing seasons and stored at 4, 8, 12, or 25ºC. Clones grown in spring had shorter dormancy than clones grown in autumn. Potato tubers grown in spring and autumn and stored at 4ºC, as well as tubers grown in autumn and stored at 8ºC had no sprouting for six months. Among clones grown in autumn, the longest dormancy period during storage at 12ºC was observed in the clone SMINIA793101-3, and at 25ºC was in the clone Asterix. In potato tubers grown in spring, the longest dormancy period during storage at 8ºC was observed in the clones SMINIA793101-3 and Missaukee, at 12ºC was in the clone SMINIA793101-3, and at 25ºC was in the clones Asterix and Missaukee. Potato tubers grown in spring had higher dry matter, starch content, and respiration rates, and lower reducing sugar, and total polyphenol content. The only exception was the Missaukee that had similar dry matter content in both growing seasons. In general, storage at 4ºC and 8ºC were more effective in reducing respiration rates and maintaining higher dry mater content of potato tubers. Chip darkening, reducing sugars, and total polyphenol content increased during storage of potato tubers. Darker chip color, higher reducing sugars and polyphenol content were obtained during storage at temperatures of 4ºC and 8ºC. These results suggest that the best storage temperature depends on genotype, tuber physiological age, and growing conditions.O objetivo deste trabalho foi determinar a qualidade de processamento dos clones de batata (Solanum tuberosum Asterix, SMINIA793101-3, e Missaukee cultivados durante outono e primavera e armazenados a 4, 8, 12, ou 25ºC. Clones produzidos na primavera tiveram menor período de dormência que clones produzidos no outono. Tubérculos produzidos na primavera e outono e armazenados a 4º

  15. Anti-ulcer activity of Ipomoea batatas tubers (sweet potato

    Directory of Open Access Journals (Sweden)

    Vandana Panda

    2012-04-01

    Full Text Available Background: Peptic ulcers occur in that part of the gastrointestinal tract which is exposed to gastric acid and pepsin, i.e., the stomach and duodenum. Gastric and duodenal ulcers are common pathologies that may be induced by a variety of factors such as stress, smoking and noxious agents including non-steroidal anti-inflammatory drugs. Ipomoea batatas tubers (sweet potato contain ample amounts of antioxidants. It has been proven already by many scientific studies that antioxidants have ulcer healing properties. In reference to this, we tried assessing the ulcer healing effect of Ipomoea batatas tubers. Methods: The anti-ulcer activity of the tubers of Ipomoea batatas (sweet potato was studied in cold stress and aspirin-induced gastric ulcers in Wistar rats. Methanolic extracts of Ipomoea batatas tubers (TE at two doses, viz., 400 and 800 mg /kg were evaluated in cold stress and aspirin-induced gastric ulcer models using cimetidine and omeprazole respectively as standards. The standard drugs and the test drugs were administered orally for 7 days in the cold stressmodel and for 1 day in the aspirin-induced gastric ulcer model. Gastroprotective potential, status of the antioxidant enzymes {superoxide dismutase (SOD, catalase (CAT, glutathione peroxidase (GPx and glutathione reductase(GR} along with GSH, and lipid peroxidation were studied in both models. Results: The results of the present study showed that TE possessed gastroprotective activity as evidenced by its significant inhibition of mean ulcer score and ulcer index and a marked increase in GSH, SOD, CAT, GPx, and GR levels and reduction in lipid peroxidation in a dose dependant manner.Conclusion: The present experimental findings suggest that tubers of Ipomoea batatas may be useful for treating peptic ulcers.

  16. Respon Pertumbuhan Tunas Kentang (Solanum tuberosum L.) Terhadap Pemberian Kinetin Secara In Vitro

    OpenAIRE

    Sitorus, Erna Meiyana

    2013-01-01

    The aim of this experiment was to know the response of potato shoot-tip growth to kinctm consentration by In Vitro method, was conduced at the Tissue Culture Laboratory, Agriculture Faculty, North Sumatera University, field research was conduced from August to October 2005. The experiment was arranged in a complete random design non factorial consist of 3 level kinetin consentrations that is Kl ( 5 mg/1 ), K2 ( 10 mg/1 ), K3 ( 15 mg/1 ). Result of experiment indicate that kinetin consentratio...

  17. Respon Pertumbuhan Dan Produksi Tanaman Kentang (Solanum tuberosum L.) Terhadap Pupuk Kalium Dan Paklobutrazol

    OpenAIRE

    Ringkas Sitepu

    2009-01-01

    The objective of the research was to know the response of growth and production of potato on potassium fertilizer and paclobutrazol. The research was done in Sukajulu, Tanah Karo North Sumatera about ±1250 metres sea level rise from June to September 2007. The research used using Randomized Block Design Factorial with two factors. The first factor was potassium fertilizer with four levels namely : 0 kg K2O/ha (K0); 0 g K2O/plot , 50 kg K2O/ha (K1); 32 g K2O/plot, 100 kg K2O/ha (K2)...

  18. Fungi colonizated of potato tubers (Solanum tuberosum L. after harvest and after storage

    Directory of Open Access Journals (Sweden)

    Bożena Cwalina-Ambrozik

    2013-12-01

    Full Text Available The aim of the laboratory experiment was the isolation of fungi colonized potato tubers directly (immediately after harvest and after 5-month storage. 67 potato tubers samples collected in 1998 and 1999 from the areas of province Warmia and Mazury were examined. The occurrence of 36 and 46 species were confirmed on analyzed tubers after harvest and storage, respectively. The most frequent species which occurred on tubers were: Alternaria alternata (9,4%, Colletotrichum cocco- des (12,6%, Rhizoctonia solani (7,4% and fungi from Fusarium genera (8,7%.This fungi, except Rhizoctonia solani, more often colonized potato tubers afier storage. Among saprofitic fungi most frequent were: Epicoccum (3,4%, Mortierella (3,1%, Mucor(6,0%, Penicillium (17,0%, Rhizopus (7,1% and Trichoderma (12,9%. More colonies of fungi were obtained from potato tubers analyzed after harvest. Remaining fungi were more frequently isolated after storage.

  19. biotechnological studies on the irradiated potato (solanum tuberosum) with gamma rays

    International Nuclear Information System (INIS)

    Bacterial wilt or brown rot disease caused by Ralstonia solanacearum causes extensive annual losses of different crops especially potato crop. It is considered as one of the limiting factors for potato production and exportation in Egypt. Therefore, the main purposes of this study were to investigate the effect of gamma rays on two potato cultivars (Diamant and Spunta). And, to obtain new genotypes of potato resistant to bacterial wilt disease. This study was carried out in the field and Biotechnology laboratory of the Plant Res. Dept., Nuclear Res. Center, Inshas, Egypt and Genetics Dept., Faculty of Agricultural., Cairo Univ., during 2008-2011. In the field experiment, dry tubers of potato cultivars were irradiated by different doses of gamma rays (20, 30 and 40 Gy) to study the effect of gamma rays on the vegetative and yield traits. The results showed that there are no significant differences between cultivars for all studied traits except a number of tubers per plant trait. Also, there are only highly significant and significant differences between treatments for weight of tubers per plant and number of tubers per plant traits, respectively. However, there are only significant differences between the interactions of cultivars and treatments for plant height and weight of tubers per plant traits. Six genotypes were selected from M1V2 generation depending on high yield for RAPD analysis to determine their genetic variability from its parents at molecular level using 11 primers. The results of RAPD analysis showed that 11 primers generated 56 distinct bands of which 31 (55.4%) were considered as polymorphic. The similarity indices of six genotypes of potato and its parents ranged from 70 to 91%. The highest genetic similarity 91% was found between D20 genotype and its parent D0 (Diamant control). On the other hand, the lowest genetic similarity 70% was found between S30, S40 genotypes and its parent S0 (Spunta control). In the artificial infection experiment under in vitro condition, the irradiated and non-irradiated plantlets of potato were cultured on medium inoculated with local virulent isolate of R. solanacearum. The results showed that all in vitro plantlets of the treatments in Diamant and Spunta cultivars were susceptible except S20 treatment was resistant to the infection with R. solanacearum. Protein analysis showed that S20 genotype (resistant mutant) displayed 2 negative unique bands that may be responsible for resistance to R. solanacearum.

  20. Biological Control Of The Egyptian Brown Rot In Potato (Solanum Tuberosum L.)

    International Nuclear Information System (INIS)

    Pseudomonas fluorescence, P. aeruginosa, Bacillus subtillus and streptomyces spp. Were used in control of Ralstonia solanacearum, the casual agent of brown rot in potato. In vitro, antagonistic activities showed that streptomyces spp. was the most antagonistic followed by P. fluorescence, Bacillus subtilus and P. aeruginosa respectively. Also, in vivo, biological control of R. solanacearum showed that Streptomyces spp. was found to reduce the percentage of brown rot infection to 5% followed by P. fluorescence, Bacillus subtilus and P. aeruginosa reducing the percentage of infection to 15 , 25 and 40%, respectively. Also, the disease severity when using Streptomyces spp. and P. fluorescence was reduced from 5 to 1 and reduced from 5 to 2 when using Bacillus subtilus and P. aeruginosa.

  1. Antioxidant Contents and Antioxidant Activities of White and Colored Potatoes (Solanum tuberosum L.)

    Science.gov (United States)

    Lee, Sang Hoon; Oh, Seung Hee; Hwang, In Guk; Kim, Hyun Young; Woo, Koan Sik; Woo, Shun Hee; Kim, Hong Sig; Lee, Junsoo; Jeong, Heon Sang

    2016-01-01

    This study was performed to evaluate and compare the antioxidant substance content and antioxidant activities of white (Superior) and colored (Hongyoung, Jayoung, Jasim, Seohong, and Jaseo) potatoes. The potatoes were extracted with 80% ethanol and were evaluated for the total polyphenol, flavonoid, and anthocyanin contents and for 1,1-diphenyl- 2-picrylhydrazyl (DPPH)/2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity, reducing power, and ferrous metal ion chelating effect. The total polyphenol, flavonoid, and anthocyanin contents of Hongyoung and Jayoung were higher than white and other colored potatoes. All colored potato extracts, except for Jaseo and Seohong, showed higher ABTS radical scavenging activities than the general white potato extract. Hongyoung and Jayoung had the highest ABTS and DPPH radical scavenging activities. Optical density values for the reducing power of Jayoung and Jaseo at concentration of 2 mg/mL were 0.148 and 0.090, respectively. All colored potato extracts had lower ferrous metal ion chelating effect than the white potato. A significant (Panthocyanin content (r=0.992), and ABTS radical scavenging activity (r=0.897). Based on these results, this research may be useful in developing the Hongyoung and Jayoung cultivars with high antioxidant activities. PMID:27390727

  2. In vitro development of buds from tubers of (Solanum tuberosum L.)

    International Nuclear Information System (INIS)

    The present work studies the in vitro development of buds from potato tubers subjected to gamma radiation at doses of 3, 6, 9 and 12 Krad. Ths effect of radiation was dependent on the dormant stage of the buds. Intermediate doses (6-9 Krad) did inhibit mitotic division but not cellular elongation. When irradiation is carried out at the end of the resting period, there is an apparent sprouting due to the elongation of previously formed cells. (Author) 17 refs

  3. Geminivirus-Mediated Genome Editing in Potato (Solanum tuberosum L.) Using Sequence-Specific Nucleases

    Science.gov (United States)

    Butler, Nathaniel M.; Baltes, Nicholas J.; Voytas, Daniel F.; Douches, David S.

    2016-01-01

    Genome editing using sequence-specific nucleases (SSNs) is rapidly being developed for genetic engineering in crop species. The utilization of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats/CRISPR-associated systems (CRISPR/Cas) for inducing double-strand breaks facilitates targeting of virtually any sequence for modification. Targeted mutagenesis via non-homologous end-joining (NHEJ) has been demonstrated extensively as being the preferred DNA repair pathway in plants. However, gene targeting via homologous recombination (HR) remains more elusive but could be a powerful tool for directed DNA repair. To overcome barriers associated with gene targeting, a geminivirus replicon (GVR) was used to deliver SSNs targeting the potato ACETOLACTATE SYNTHASE1 (ALS1) gene and repair templates designed to incorporate herbicide-inhibiting point mutations within the ALS1 locus. Transformed events modified with GVRs held point mutations that were capable of supporting a reduced herbicide susceptibility phenotype, while events transformed with conventional T-DNAs held no detectable mutations and were similar to wild-type. Regeneration of transformed events improved detection of point mutations that supported a stronger reduced herbicide susceptibility phenotype. These results demonstrate the use of geminiviruses for delivering genome editing reagents in plant species, and a novel approach to gene targeting in a vegetatively propagated species. PMID:27493650

  4. Geminivirus-Mediated Genome Editing in Potato (Solanum tuberosum L.) Using Sequence-Specific Nucleases.

    Science.gov (United States)

    Butler, Nathaniel M; Baltes, Nicholas J; Voytas, Daniel F; Douches, David S

    2016-01-01

    Genome editing using sequence-specific nucleases (SSNs) is rapidly being developed for genetic engineering in crop species. The utilization of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats/CRISPR-associated systems (CRISPR/Cas) for inducing double-strand breaks facilitates targeting of virtually any sequence for modification. Targeted mutagenesis via non-homologous end-joining (NHEJ) has been demonstrated extensively as being the preferred DNA repair pathway in plants. However, gene targeting via homologous recombination (HR) remains more elusive but could be a powerful tool for directed DNA repair. To overcome barriers associated with gene targeting, a geminivirus replicon (GVR) was used to deliver SSNs targeting the potato ACETOLACTATE SYNTHASE1 (ALS1) gene and repair templates designed to incorporate herbicide-inhibiting point mutations within the ALS1 locus. Transformed events modified with GVRs held point mutations that were capable of supporting a reduced herbicide susceptibility phenotype, while events transformed with conventional T-DNAs held no detectable mutations and were similar to wild-type. Regeneration of transformed events improved detection of point mutations that supported a stronger reduced herbicide susceptibility phenotype. These results demonstrate the use of geminiviruses for delivering genome editing reagents in plant species, and a novel approach to gene targeting in a vegetatively propagated species. PMID:27493650

  5. PRODUKSI BIBIT KENTANG (Solanum tuberosum L.) G1 DARI STEK BATANG

    OpenAIRE

    Putu Wina Andriani Lestari; Made Ria Defiani; Ida Ayu Astarini

    2014-01-01

    This research was conducted to find out the best media and auxin for plantingpotato cutting to produce G1 seed potato. Research was done at a shade house ofKebun Bibit Hortikultura Kembang Merta, Candi Kuning Village, Baturiti, TabananRegency, Bali. Were planted at the screen house for sprouting. Four weeks old ofshoots were used as cutting. Each cutting measured 10 cm. Stem cutting of G0 seedpotatoes was dipped in 2 different auxin types (Rootone F and auxin paste) and plantedin 2 different ...

  6. Genetic Diversity and Yield Stability of Potato(Solanum tuberosum L.)

    Institute of Scientific and Technical Information of China (English)

    Biswas M K; Hossain M; Islam R

    2008-01-01

    The present study was under taken to investigate the genetic distance and the performance of thirty one potato genotypes in diverse environments. Multivariate and canonical analysis were used to study genetic diversity among the genotypes and stability parameters were estimated according to Eberhart and Russell, Parkinge and Jinks and Freeman and Perkins models for plant height, branch number per plant, tuber number and tuber weight. Thirty one genotypes were grouped in to six clusters with the help of Mahalonobis distance and canonical analysis. The stability analysis revealed that yield was greatly influenced by the environments. Most of the studied genotypes were sensitive for yield with the environ-ment. For over-all performance 'Hera','Chamak','Patnai','Lala pekri','TPS-7', 'TPS-364', 'Somerset' and 'Superi-or' were the best genotypes for tuber yield and breeding material for potato breeding.

  7. Antioxidant Contents and Antioxidant Activities of White and Colored Potatoes (Solanum tuberosum L.).

    Science.gov (United States)

    Lee, Sang Hoon; Oh, Seung Hee; Hwang, In Guk; Kim, Hyun Young; Woo, Koan Sik; Woo, Shun Hee; Kim, Hong Sig; Lee, Junsoo; Jeong, Heon Sang

    2016-06-01

    This study was performed to evaluate and compare the antioxidant substance content and antioxidant activities of white (Superior) and colored (Hongyoung, Jayoung, Jasim, Seohong, and Jaseo) potatoes. The potatoes were extracted with 80% ethanol and were evaluated for the total polyphenol, flavonoid, and anthocyanin contents and for 1,1-diphenyl- 2-picrylhydrazyl (DPPH)/2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity, reducing power, and ferrous metal ion chelating effect. The total polyphenol, flavonoid, and anthocyanin contents of Hongyoung and Jayoung were higher than white and other colored potatoes. All colored potato extracts, except for Jaseo and Seohong, showed higher ABTS radical scavenging activities than the general white potato extract. Hongyoung and Jayoung had the highest ABTS and DPPH radical scavenging activities. Optical density values for the reducing power of Jayoung and Jaseo at concentration of 2 mg/mL were 0.148 and 0.090, respectively. All colored potato extracts had lower ferrous metal ion chelating effect than the white potato. A significant (P<0.05) positive correlation was observed between total polyphenol content and total flavonoid content (r=0.919), anthocyanin content (r=0.992), and ABTS radical scavenging activity (r=0.897). Based on these results, this research may be useful in developing the Hongyoung and Jayoung cultivars with high antioxidant activities. PMID:27390727

  8. Cadmium Induced Changes in Metabolic Function of Mitochondrial Isolated from Potato Tissue (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Chagra Ali

    2009-01-01

    Full Text Available Problem statement: Cadmium is highly toxic at low concentrations, but the mechanism of its toxicity is still not understood particularly at the cellular and subcellular level. Approach: In this study we examined the effects of cadmium on the oxidophosphorylation properties of mitochondria isolated from potatoes. Results: Cadmium strongly disturbed the respiratory metabolism of mitochondria isolated especially in the transfer of electrons by cyanide pathway. Meanwhile, cadmium altered the composition of lipid fatty acids polar while inhibiting catalase activity, a key enzyme in the detoxification (antioxidant process. In addition, cadmium caused an increase in mitochondrial volume associated with strong inhibition of ATPase activity, which could be explained by a transport of the potassium ion stimulation at the origin of the massive influx of H+ by antiport through the K+/H+ leading to a decoupling (cut of mitochondrial oxidative phosphorylation. The swelling of mitochondria was accompanied by the rupture of the mitochondrial outer membrane and thus the release of Cytochrome C, which appears to be the initial phase of apoptosis. Conclusion: Following this study, it appeared that cadmium generates in potato the isolated mitochondria a concentration-dependent oxidative stress.

  9. Mechanisms of self-incompatibility and unilateral incompatibility in diploid potato (Solanum tuberosum L.).

    NARCIS (Netherlands)

    Eijlander, R.

    1998-01-01

    In chapter 1 an overview is given of the major mechanisms operating in Angiosperms that prevent or limit the degree of inbreeding. The two major systems that function on the basis of interaction between pollen and stigma/style, are the sporophytic and the gametophytic self-incompatibility systems (S

  10. Agronomical indicators for determination of potato (Solanum tuberosum L. tolerance to drought

    Directory of Open Access Journals (Sweden)

    Alfredo Morales Rodríguez

    2016-01-01

    Full Text Available Lately, water shortage has caused 50% losses in potato yields in several Central American countries. Knowledge and use of more tolerant potato varieties are a critical element to increase production and yields under stressing conditions. The experiments were developed at the Center for Tropical Crops Research (INIVIT, between December 2014, and March 2015. Ten plots were planted, including different varieties. Half of them (5 were not irrigated for 20 days, starting on the 50th day after plantation (drought stress period. Irrigation was resumed on the 70th day. The Stress Susceptibility Index (SSI, Stress Tolerance Index (STI, Tolerance Index (TOL, Mean Productivity (MP, and Yield Stability Index (YSI. The Atlas and Maranca varieties had the lowest values for SSI (0.67 and 0.61, respectively, and the highest values for STI (0.79 and 0.81, respectively. The highest MP (19.92 t/ha was achieved by Atlas. The highest YSI values were observed in Maranca and Atlas (81.07 and 79.29, respectively.

  11. Preservation by Irradiation of three varieties of Colombian Potatoes (Solanum Tuberosum L.)

    International Nuclear Information System (INIS)

    This is first study performed in the Gamma Facility of the Instituto de Asuntos Nucleares, in Bogota, Colombia, related to the Food Irradiation Programme. The study was made with: Parda Pastusa, ICA Guantiva, and ICA Purace, selected as the most important varieties of colombian potatoes. A complete study prior to the irradiation exposure was made in order to determine the optimum irradiation package. Different dose treatments, chemical and physical changes and organoleptic tests, were studied in this work. The work was performed in the early 1980s

  12. Species of Pratylenchus Associated with Solanum tuberosum cv Superior in Ohio

    OpenAIRE

    Brown, M.J.; Riedel, R. M.; Rowe, R C

    1980-01-01

    Seventy-three Ohio fields comprising ca. 440 ha of cv Superior potatoes were surveyed in 1977 for plant-parasitic nematodes. Of eight genera of plant-parasitic nematodes, Pratylenchus was found most frequently, occurring in 65% of the soil samples and 84% of the root samples. Populations of Pratylenchus were consistently higher than populations of the other nematode genera. The six species of Pratylenchus extracted from potato roots, in descending order of frequency, were P. crenatus, P. pene...

  13. The Critical Period for Weed Control (CPWC) in Potato (Solanum tuberosum L.)

    OpenAIRE

    Dogan ISIK; Adem AKCA; KAYA ALTOP, Emine; TURSUN, Nihat; Husrev MENNAN

    2015-01-01

    Accurate assessment of crop-weed control period is an essential part for planning an effective weed management for cropping systems. Field experiments were conducted during the seasonal growing periods of potato in 2012 and 2013 in Kayseri, Turkey to assess critical period for weed control (CPWC) in potato. A four parameter log-logistic model was used to assist in monitoring and analysing two sets of related, relative crop yield. Data was obtained during the periods of increased weed interfer...

  14. Variedades de batatinha (Solanum tuberosum L. procedentes da Polônia

    Directory of Open Access Journals (Sweden)

    O. J. Boock

    1951-12-01

    Full Text Available As variedades de batatinha Dar, Oka, Bem, Pionier e Kolobrzeskie, foram recebidas da Polônia, para verificação do seu comportamento sob as nossas condições, em confronto com a variedade "Eigenheimer", já conhecida (1. Com êsse objetivo, foram instaladas oito experiências, sendo três em Capão Bonito, em solo sílico-argiloso, três em Louveira, em salmourão, e duas em Indaiatuba, em terreno sílico-argiloso. Das observações realizadas durante o período vegetativo, e dos resultados da produção, conclui-se o seguinte : a tôdas as variedades apresentaram película amarelada e que se tornou verde à ação da luz ; b a folhagem da variedade Kolobrzeskie se mostrou de um verde-claro e de porte médio, contrastando com a Pionier, que se mostrou de um verde forte e porte avantajado ; c a Kolobrzeskie e a Pionier revelaram boa resistência à Phytophthora injestans e Alternaria solani, principalmente quando comparadas à "Eigenheimer", ao passo que a Oka mostrou ser pouco resistente ; d a variedade Bem se mostrou mais suscetível às manchas internas de orígem fisiológica (chocolate, sendo que a Pionier revelou pequena tendência a êsse defeito ; e as variedades mais produtivas foram, por ordem decrescente (média de 6 experiências : "Eigenheimer" Oka, Kolobrzeskie, Bem, Pionier e Dar; f as maiores percentagens de tubérculos graúdos foram fornecidas pela Kolobrzeskie e Bem, e, de refugo, pelas variedades Oka, Dar e "Eigenheimer".Seed tubers of Irish potato varieties Dar, Oka, Bern, Pionier and Kolobrzeskie, received from Poland, were tested in the State of São Paulo with the best known Dutch variety, Eigenheimer. Eight trials were conducted in three different localities : Capão Bonito, Louveira and Indaiatuba. Varieties Kolobrzeskie and Pionier showed good resistance to both early and late blight. On the contrary, the varieties Eigenheimer and Oka were susceptible. Variety Bem has shown some physiological disorders through spotting of tuber flesh. Little tendency to such disorders was revealed by variety Pionier. The best yielding varieties were Eigenheimer, Oka, Kolobrzeskie, Bern, Pionier and Dar. Variety Kolobrzeskie and Bem yielded larger percentage of big-sized tubers;. Oka, Dar and Eigenheimer produced larger percentages of small-sized tubers.

  15. The Critical Period for Weed Control (CPWC in Potato (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Dogan ISIK

    2015-12-01

    Full Text Available Accurate assessment of crop-weed control period is an essential part for planning an effective weed management for cropping systems. Field experiments were conducted during the seasonal growing periods of potato in 2012 and 2013 in Kayseri, Turkey to assess critical period for weed control (CPWC in potato. A four parameter log-logistic model was used to assist in monitoring and analysing two sets of related, relative crop yield. Data was obtained during the periods of increased weed interference and as a comparison, during weed-free periods. In both years, the relative yield of potato decreased with a longer period of weed-interference whereas increased with increasing length of weed free period. In 2012, the CPWC ranged from 112 to 1014 GDD (Growing Degree Days which corresponded to 8 to 66 days after crop emergence (DAE and between 135-958 GDD (10 to 63 DAE in the following year based on a 5% acceptable yield loss. Weed-free conditions needed to be established as early as the first week after crop emergence and maintained as late as ten weeks after crop emergence to avoid more than 5% yield loss in the potato. The results suggest that CPWC could well assist potato producers to significantly reduce the expense of their weed management programs as well as improving its efficacy.

  16. Plant regeneration from in vitro-selected salt tolerant callus cultures of solanum tuberosum

    International Nuclear Information System (INIS)

    This research work reports In vitro direct selection of salt-tolerant callus cultures and subsequent plant regeneration in two potato cultivars (Cardinal and Desiree). Results have shown more than 50% reduction in relative fresh callus mass in the two potato cultivars exposed to 120 mM NaCl. Callus morphology correspondingly changed from off-white to blackish-brown at 120 mM to acutely-necrotic at 140 mM NaCl. Regeneration potential of recurrently-selected callus cultures (100 mM NaCl-treated) on salt-free regeneration medium (MS + 2.64 meu M NAA and 1.00 meu M TDZ) was not much different as compared to the control (non-selected ones). Regenerated plants from salt-tolerant callus cultures of both the cultivars after selection were transferred to soil and organic matter (50:50, v/v) for acclimatization in the greenhouse. It was observed that the recurrently-selected plants had higher fresh/dry weight and number of tubers compared with the control ones in both the cultivars. Likewise the protein, peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) activities have shown an increasing trend in salt-treated plants of both the cultivars. The results from this study highlighted a strong possibility for the selection of salt-tolerant callus lines of potato followed by an efficient plant regeneration and further acclimatization. (author)

  17. Purple potato (Solanum tuberosum L.) anthocyanins attenuate alcohol-induced hepatic injury by enhancing antioxidant defense.

    Science.gov (United States)

    Jiang, Zhihui; Chen, Chen; Wang, Jian; Xie, Wenyan; Wang, Meng; Li, Xinsheng; Zhang, Xiaoying

    2016-01-01

    Alcoholic liver disease (ALD) is a serious and challenging health issue. In the past decade, natural components possessing hepatoprotective properties have gained more attention for ALD intervention. In this study, the phytochemical components of anthocyanins from purple potato were assessed using UPLC-MS/MS, and the hepatoprotective effects of purple potato anthocyanins (PPAs) were investigated in the ALD mouse model. Serum and liver biochemical parameters were determined, along with histopathological changes in liver tissue. In addition, the major contributors to alcohol-induced oxidative stress were assessed. The results indicated that the levels of aspartate transaminase and alanine transaminase were lower in the serum of the PPA-treated group than the alcohol-treated group. PPAs significantly inhibited the reduction of total cholesterol and triglycerides. Higher levels of superoxide dismutase and reduced glutathione enzymes as well as a reduction in the formation of malondialdehyde occurred in mice fed with PPAs. In addition, PPAs protected against increased alcohol-induced levels and activity of cytochrome P450 2E1 (CYP2E1), which demonstrates the effects of PPAs against alcohol-induced oxidative stress and liver injury. This study suggests that PPAs could be an effective therapeutic agent in alcohol-induced liver injuries by inhibiting CYP2E1 expression and thereby strengthening antioxidant defenses. PMID:26481011

  18. Ultrasound-assisted three-phase partitioning of polyphenol oxidase from potato peel (Solanum tuberosum).

    Science.gov (United States)

    Niphadkar, Sonali S; Rathod, Virendra K

    2015-01-01

    Conventional three phase partitioning (TPP) and ultrasound assisted three phase partitioning (UATPP) were optimized for achieving the maximum extraction and purification of polyphenol oxidase (PPO) from waste potato peels. Different process parameters such as ammonium sulfate (NH4)2SO4 concentration, crude extract to t-butanol ratio, time, temperature and pH were studied for conventional TPP. Except agitation speed, the similar parameters were also optimized for UATPP. Further additional parameters were also studied for UATPP viz. irradiation time at different frequencies, duty cycle and, rated power in order to obtain the maximum purification factor and recovery of PPO. The optimized conditions for conventional TPP were (NH4)2SO4 0-40% (w/v), extract to t-butanol ratio 1:1 (v/v), time 40 min and pH 7 at 30°C. These conditions provided 6.3 purification factor and 70% recovery of PPO from bottom phase. On the other hand, UATPP gives maximum purification fold of 19.7 with 98.3% recovery under optimized parameters which includes (NH4)2SO4 0-40% (w/v), crude extract to t-butanol ratio 1: 1 (v/v) pH 7, irradiation time 5 min with 25 kHz, duty cycle 40% and rated power 150W at 30°C. UATPP delivers higher purification factor and % recovery of PPO along with reduced operation time from 40 min to 5 min when compared with TPP. SDS PAGE showed partial purification of PPO enzyme with UATPP with molecular weight in the range of 26-36 kDa. Results reveal that UATPP would be an attractive option for the isolation and purification of PPO without need of multiple steps. PMID:26139472

  19. Interaction of polyphenol oxidase of Solanum tuberosum with β-cyclodextrin: Process details and applications.

    Science.gov (United States)

    Singh, Virendra; Jadhav, Swati B; Singhal, Rekha S

    2015-09-01

    Polysaccharides differing in structure and chemical nature were screened for their ability to bind non-covalently with polyphenol oxidase (PPO) from potato (as a model) and their effect on enzyme activity. All the polysaccharides selected inhibited the PPO but β-cyclodextrin showed maximum inhibition under optimum conditions. Process details for the inhibition of PPO were studied with respect to concentration of β-cyclodextrin, temperature, pH, and time. Higher inhibition constant and lower half life was obtained at 40 °C than at 30 °C in the presence of inhibitor. β-Cyclodextrin showed mixed type of inhibition of PPO. β-Cyclodextrin was further exploited as anti-browning agent in selected fruit juices. It not only showed a significant anti-browning effect on freshly prepared potato juice but was also effective in other fruit juices. Better effect was seen in pineapple, apple and pear as compared to banana, sugarcane and guava fruit juices. PMID:26187193

  20. Expression of human dopamine receptor in potato (Solanum tuberosum results in altered tuber carbon metabolism

    Directory of Open Access Journals (Sweden)

    Świędrych Anna

    2005-02-01

    Full Text Available Abstract Background Even though the catecholamines (dopamine, norepinephrine and epinephrine have been detected in plants their role is poorly documented. Correlations between norepinephrine, soluble sugars and starch concentration have been recently reported for potato plants over-expressing tyrosine decarboxylase, the enzyme mediating the first step of catecholamine synthesis. More recently norepinephrine level was shown to significantly increase after osmotic stress, abscisic acid treatment and wounding. Therefore, it is possible that catecholamines might play a role in plant stress responses by modulating primary carbon metabolism, possibly by a mechanism similar to that in animal cells. Since to date no catecholamine receptor has been identified in plants we transformed potato plants with a cDNA encoding human dopamine receptor (HD1. Results Tuber analysis of transgenic plants revealed changes in the activities of key enzymes mediating sucrose to starch conversion (ADP-glucose phosphorylase and sucrose synthase and sucrose synthesis (sucrose phosphate synthase leading to altered content of both soluble sugars and starch. Surprisingly the catecholamine level measured in transgenic plants was significantly increased; the reason for this is as yet unknown. However the presence of the receptor affected a broader range of enzyme activities than those affected by the massive accumulation of norepinephrine reported for plants over-expressing tyrosine decarboxylase. Therefore, it is suggested that the presence of the exogenous receptor activates catecholamine cAMP signalling in plants. Conclusions Our data support the possible involvement of catecholamines in regulating plant carbon metabolism via cAMP signalling pathway.

  1. Hydroxycinnamic acid functional ingredients and their biosynthetic genes in tubers of Solanum tuberosum Group Phureja

    Directory of Open Access Journals (Sweden)

    Liyao Ji

    2016-12-01

    Full Text Available Potato is an ideal candidate for the delivery of functional ingredients due to its high worldwide consumption. The metabolites in cooked tubers of eight diploid potato genotypes from Colombia were explored. Potato tubers were harvested, cooked,lyophilized, and then stored at −80°C. Metabolites were extracted from flesh samples and analyzed using liquid chromatography and high-resolution mass spectrometry. A total of 294 metabolites were putatively identified, of which 87 metabolites were associated with health-benefiting roles for humans, such as anticancer and anti-inflammatory properties. Two metabolites, chlorogenic acid and N-Feruloyltyramine were detected in high abundance and were mapped on to the potato metabolic pathways to predict the related biosynthetic enzymes: hydroxycinnamoyl-CoA quinate transferase (HQT and tyramine hydroxycinnamoyl transferase (THT, respectively. The coding genes of these enzymes identified nonsynonymous single-nucleotide polymorphisms (nsSNPs in AC09, AC64, and Russet Burbank, with the highest enzyme stability found in AC09. This is consistent with the highest presence of hydroxycinnamic acids in the AC09 genotype. The metabolites detected at high fold change, their functional ingredient properties, and their enhancement through breeding to improve health of the indigenous communities’ of Colombia are discussed.

  2. Processing of pigmented-flesh potatoes (Solanum tuberosum L.) on the retention of bioactive compouns

    Science.gov (United States)

    Antioxidant retention in white-, yellow-, red-, and purple-flesh potato cultivars after drum drying (DD), freeze drying (FD) and Refractance Window™ drying (RW) was compared. Dried potatoes were analyzed for total antioxidant activity, phenolics, anthocyanins and carotenoids. Total phenolics were hi...

  3. Optimisation of antioxidant extraction from Solanum tuberosum potato peel waste by surface response methodology

    OpenAIRE

    Rodríguez-Amado, I.; Franco, Daniel; Sánchez, Maribel; Zapata, Carlos; Vázquez, José Antonio

    2014-01-01

    This study reports the optimised conditions (temperature, ethanol concentration and processing-time) for antioxidant extraction from potato peel (Agria variety) waste. At short extraction times (34 min), optimal yields of phenolic (TP) and flavonoid (Fv) compounds were reached at 89.9 °C and ethanol concentrations of 71.2% and 38.6%, respectively. The main phenolic compounds identified in the extracts were chlorogenic (Cl) and ferulic (Fer) acids. A significant positive correlation was found ...

  4. Optimisation of antioxidant extraction from Solanum tuberosum potato peel waste by surface response methodology.

    Science.gov (United States)

    Amado, Isabel Rodríguez; Franco, Daniel; Sánchez, Marivel; Zapata, Carlos; Vázquez, José Antonio

    2014-12-15

    This study reports the optimised conditions (temperature, ethanol concentration and processing-time) for antioxidant extraction from potato peel (Agria variety) waste. At short extraction times (34 min), optimal yields of phenolic (TP) and flavonoid (Fv) compounds were reached at 89.9°C and ethanol concentrations of 71.2% and 38.6%, respectively. The main phenolic compounds identified in the extracts were chlorogenic (Cl) and ferulic (Fer) acids. A significant positive correlation was found between antioxidant activity and TP, Fv, Fer and Cl responses. Potato peel extracts were able to stabilize soybean oil under accelerated oxidation conditions, minimising peroxide, totox and p-anisidine indices. The production of hexanal and 2-hexenal in soybean oil samples was maximal for extracts obtained at intermediate temperatures and ethanol concentrations. Our results demonstrate potato peel waste is a good source of antioxidants able to effectively limit oil oxidation, while contributing to the revalorisation of these agrifood by-products. PMID:25038678

  5. Characterization of promising potato clones (solanum tuberosum l. subspecies andigena) for starch extraction

    OpenAIRE

    Garnica Holguin, Ana Magdalena; Romero Bernal, Angela Rocio; Prieto Contreras, Lena Food Engineering Program, Faculty of Eng; Ceron Lasso, Maria Del Socorro; Argüelles Cárdenas, Jorge

    2013-01-01

    Colombia has been overproducing potatoes with around 18% remaining unmarketable, constituting a potential alternative use in obtaining native starch for the food industry. To this end, 17 promising potato clones from the Programa de Mejoramiento Genetico of Corpoica were characterized for agronomic variables, as well as physicochemical variables for the tubers. The results were analyzed with descriptive statistics, Pearson correlation and cluster analysis. Clone codified 36 was selected as ha...

  6. Effect of hydrogen peroxide and thiourea on fluorescence and tuberization of potato (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Mani F.

    2012-08-01

    Full Text Available The aim of this study is to determine the effect of hydrogen peroxide and thiourea on potato crop (quantum yield (Fv/ Fm, chlorophyll content, tuber diameter, tuber number and total tuber yield. The concentrations of these two chemicals are hydrogen peroxide: 0, 20, 40, 60 and 80 mM, and thiourea : 0, 250, 500, 750 and 1000 mM. The experiment was conducted in the farm of Chott-Mariem Institute during three months using variety 'Spunta' and arranged in a completely randomized block with three replications. Results show that there is no significant difference in tuber diameter between treatments and among the same treatment. However, tuber yield is significantly increased by 20 % by thiourea (250 mM. Maximum total yield was obtained at this concentration (810 g/plant. In addition, application of thiourea (500 and 750 mM results in a significantly higher number of tubers number (5.7 and 5.2 respectively. In contrast, treatment with hydrogen peroxide brings about similar tuber yields. Although, application of hydrogen peroxide at low concentration (20 mM, decreases chlorophyll content and stresses plants, application of thiourea increases chlorophyll content, and improve quantum yield especially when it is applied at 250 mM.

  7. Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, Solanum tuberosum

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    Munger Aurélie

    2012-11-01

    Full Text Available Abstract Background Studies reported unintended pleiotropic effects for a number of pesticidal proteins ectopically expressed in transgenic crops, but the nature and significance of such effects in planta remain poorly understood. Here we assessed the effects of corn cystatin II (CCII, a potent inhibitor of C1A cysteine (Cys proteases considered for insect and pathogen control, on the leaf proteome and pathogen resistance status of potato lines constitutively expressing this protein. Results The leaf proteome of lines accumulating CCII at different levels was resolved by 2-dimensional gel electrophoresis and compared with the leaf proteome of a control (parental line. Out of ca. 700 proteins monitored on 2-D gels, 23 were significantly up- or downregulated in CCII-expressing leaves, including 14 proteins detected de novo or up-regulated by more than five-fold compared to the control. Most up-regulated proteins were abiotic or biotic stress-responsive proteins, including different secretory peroxidases, wound inducible protease inhibitors and pathogenesis-related proteins. Accordingly, infection of leaf tissues by the fungal necrotroph Botryris cinerea was prevented in CCII-expressing plants, despite a null impact of CCII on growth of this pathogen and the absence of extracellular Cys protease targets for the inhibitor. Conclusions These data point to the onset of pleiotropic effects altering the leaf proteome in transgenic plants expressing recombinant protease inhibitors. They also show the potential of these proteins as ectopic modulators of stress responses in planta, useful to engineer biotic or abiotic stress tolerance in crop plants of economic significance.

  8. Pathogens of potato (Solanum tuberosum L. tuber (Phytophtora infestans occurring in treatments with foliar fertilization

    Directory of Open Access Journals (Sweden)

    Bożena Cwalina-Ambroziak

    2012-10-01

    Full Text Available The paper presents the results of a three-year exact plot experiment (2008-2010 established in Bałcyny (NE Poland. Three potato cultivars were grown: medium-early ‘Adam’, medium-late ‘Pasja Pomorska’, and late ‘Ślęza’. The experimental factors were foliar fertilizers applied alone or in combination (Basfoliar 12-4-6, ADOB Mn, Solubor DF and two levels of soil mineral fertilization (N1P1K1-80 kg N × ha-1, 80 kg P × ha-1, 120 K × ha-1; N2P2K2-120 kg N × ha-1, 144 kg P × ha-1, 156 K × ha-1. The experimental materials comprised potato tubers. The symptoms of soft rot (Pectobacterium carotovorum subsp. carotovorum, late blight (Phytophthora infestans and dry rot (Fusarium spp. were evaluated in 5 kg potato samples, and were expressed as the percentage mass of infected tubers. The rates of common scab (Streptomyces scabies and black scurf (Rhizoctonia solani infection were estimated on 100 tubers collected randomly after harvest, according to a nine-point scale, and were presented as a percentage infection index. In the laboratory, fungi were isolated on PDA medium from potato tubers immediately after harvest and after five-month storage. The incidence of tuber diseases depended on potato cultivars affected. The severity of tuber diseases varied between treatments with two levels of NPK fertilization and foliar fertilization. The lowest number of Fusarium-infected tubers was obtained from treatments where three foliar fertilizers were applied in combination, which was confirmed by the lowest abundance of fungal isolates. More fungi were isolated from potato tubers after harvest than after storage, but pathogens were more frequently isolated from stored tubers. After harvest, the lowest number of pathogenic fungi was isolated from the tubers of cv. ‘Adam’ in the non-fertilized treatment, and after storage – from the tubers of the late cultivars in the treatment with three foliar fertilizers applied in combination.

  9. Fungi colonizated of potato tubers (Solanum tuberosum L.) after harvest and after storage

    OpenAIRE

    Bożena Cwalina-Ambrozik

    2013-01-01

    The aim of the laboratory experiment was the isolation of fungi colonized potato tubers directly (immediately) after harvest and after 5-month storage. 67 potato tubers samples collected in 1998 and 1999 from the areas of province Warmia and Mazury were examined. The occurrence of 36 and 46 species were confirmed on analyzed tubers after harvest and storage, respectively. The most frequent species which occurred on tubers were: Alternaria alternata (9,4%), Colletotrichum cocco- des (12,6%)...

  10. Pathogens of potato (Solanum tuberosum L.) tuber (Phytophtora infestans) occurring in treatments with foliar fertilization

    OpenAIRE

    Bożena Cwalina-Ambroziak; Bożena Bogucka

    2012-01-01

    The paper presents the results of a three-year exact plot experiment (2008-2010) established in Bałcyny (NE Poland). Three potato cultivars were grown: medium-early ‘Adam’, medium-late ‘Pasja Pomorska’, and late ‘Ślęza’. The experimental factors were foliar fertilizers applied alone or in combination (Basfoliar 12-4-6, ADOB Mn, Solubor DF) and two levels of soil mineral fertilization (N1P1K1-80 kg N × ha-1, 80 kg P × ha-1, 120 K × ha-1; N2P2K2-120 kg N × ha-1, 144 kg P × ha-1, 156 K × ha-1). ...

  11. Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs.

    Science.gov (United States)

    Chi, Ming; Bhagwat, Basdeo; Tang, Guiliang; Xiang, Yu

    2016-01-01

    It is of great importance and interest to develop crop varieties with low polyphenol oxidase (PPO) activity for the food industry because PPO-mediated oxidative browning is a main cause of post-harvest deterioration and quality loss of fresh produce and processed foods. We recently demonstrated that potato tubers with reduced browning phenotypes can be produced by inhibition of the expression of several PPO gene isoforms using artificial microRNA (amiRNA) technology. The approach introduces a single type of 21-nucleotide RNA population to guide silencing of the PPO gene transcripts in potato tissues. Some advantages of the technology are: small RNA molecules are genetically transformed, off-target gene silencing can be avoided or minimized at the stage of amiRNA designs, and accuracy and efficiency of the processes can be detected at every step using molecular biological techniques. Here we describe the methods for transformation and regeneration of potatoes with amiRNA vectors, detection of the expression of amiRNAs, identification of the cleaved product of the target gene transcripts, and assay of the expression level of PPO gene isoforms in potatoes. PMID:26843174

  12. Varietal and environmental effect on the glycoalkaloid content of potato (Solanum tuberosum L.).

    Science.gov (United States)

    Uppal, D S

    1987-01-01

    The tubers of 14 potato varieties were analysed for glycoalkaloids. The level of glycoalkaloids in tubers of all the varieties analysed are within the safe limits for human consumption. The peels of tuber contained about 60-70% of the total glycoalkaloids present in the whole tuber. The levels of glycoalkaloids in leaves and tubers were correlated (r = 0.865). There was a significant increase in the content of glycoalkaloids in peels of tubers exposed to sunlight. Glycoalkaloid contents increased at the rate of 1.9 mg/100 g fresh weight per day in peels of "Kufri Jyoti" tubers exposed to diffused sunlight. PMID:3507005

  13. Occurrence of Tomato spotted wilt virus in Stevia rebaudiana and Solanum tuberosum in Northern Greece

    NARCIS (Netherlands)

    Chatzivassiliou, E.K.; Peters, D.; Lolas, P.

    2007-01-01

    Tomato spotted wilt virus (TSWV) (genus Tospovirus, family Bunyaviridae) was first reported in Greece during 1972 (3) and currently is widespread in the central and northern part of the country infecting several cultivated and wild plant species (1,2). In June 2006, virus-like symptoms similar to th

  14. INTRASPECIFIC VARIABILITY IN TETRAPLOID VARIETIES (4X OF POTATO (Solanum tuberosum L.

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    J. Castillo

    2001-01-01

    Full Text Available En el Instituto Nacional de Ciencias Agrícolas (INCA se estudiaron durante dos campañas (1997/1998, 1998/ 1999 seis cruzamientos intraespecíficos, entre tres variedades comerciales de papa -Desirée, Atlantic y Kondor- y cuatro clones provenientes del Centro Internacional de la Papa -CIP 23, 110, 114 y 115- mediante técnicas electroforéticas, con el objetivo de determinar la variabilidad genética originada en ellos y la posibilidad de su utilización en el programa de mejoramiento del cultivo. Se utilizaron dos sistemas izoenzimáticos, las esterasas y peroxidasas. Se tomaron dieciocho muestras por cada cruce, conjuntamente con los progenitores, de la primera hoja verdadera de las plántulas. Se realizó un análisis cualitativo por la presencia o ausencia de bandas y un análisis cuantitativo para el cálculo del índice de similitud de Jaccard. Con los valores totales del índice de similitud para ambos sistemas se realizó una prueba ¿t¿ para determinar si existían diferencias significativas entre los cruzamientos. Se encontró más variabilidad dentro de cada cruce que entre ellos, destacándose los cruces Desirée x CIP 23, Desirée x CIP 115 y Desirée x CIP 110. Se pudo constatar que el sistema esterasa mostró un elevado polimorfismo, detectando una mayor variabilidad que el sistema peroxidasa.

  15. Occurrence and physico-chemical properties of protease inhibitors from potato tuber (Solanum tuberosum)

    NARCIS (Netherlands)

    Pouvreau, L.A.M.

    2004-01-01

    Potato proteins are present in a by-product of the potato starch industry, the so-called potato juice. They are recovered by an acidic heat-treatment of the potato juice. This results in a completely irreversible precipitation of the proteins, with a complete loss of functionality for food applicati

  16. Accumulation and localization of cadmium in potato (Solanum tuberosum) under different soil Cd levels.

    Science.gov (United States)

    Chen, Zhifan; Zhao, Ye; Gu, Lei; Wang, Shuifeng; Li, Yongliang; Dong, Fangli

    2014-06-01

    Phytoavailability and uptake mechanism of Cd in edible plant tissues grown on metal polluted agricultural soils has become a growing concern worldwide. Uptake, transport, accumulation and localization of cadmium in potato organs under different soil Cd levels were investigated using inductively-coupled plasma mass spectrometry and energy dispersive X-ray microanalysis. Results indicated that Cd contents in potato organs increased with increasing soil Cd concentrations, and the order of Cd contents in different organs was leaves > stems/roots > tubers. Root-to-stem Cd translocation coefficients ranged from 0.89 to 1.81. Cd localization in potato tissues suggested that leaves and stems should be the main compartment of Cd storage and uptake. Although low concentrations of Cd migrated from the root to tuber, Cd accumulation in the tuber exceeded the standard for food security. Therefore, the planting of potato plants in farmland containing Cd should be closely evaluated due to its potential to present health risks. PMID:24682567

  17. Irradiations with fast neutrons induce salinity tolerance in potato,Solanum tuberosum L., callus

    International Nuclear Information System (INIS)

    Sterilized leaf explants were excised from 30-days old potato plants and cut into discs (1 cm. each). The discs grown under tissue culture techniques using MS-medium (Murashige and Skoog, 1962), supplemented with 0.3 mg.L. 1 2,4-0 and 3.0 mg.L. 1 kinetin. Cultures were incubated at 22:±:2 .ºC in a complete darkness for callus induction. Three weeks later, calli were removed and exposed to different doses of fast neutrons (10. , 10. , 10. , 10. and 10. n/cm. The control (discs not treated with fast neutrons) and fast neutrons irradiated calli were subcultured on MS media for 5 weeks and left to grow at 22 .ºC under low light intensity (1500 Lux, daily fluorescent tubes). Callus irradiated with 10. n/cm. fast neutrons produced the highest yield on salt free medium as compared with the control sample. Calli treated with fast neutrons as well as the control were subcultured on MS media containing various concentrations (0.0, 50,100, 150 and 200 mM) of NaCI. The potato calli exposed to 10. or 10. n/cm. fast neutrons exhibited more salinity tolerance, and were able to continue their growth even under the higher level of salinity (200 mM) NaCI throughout 6 weeks of growth. However, calli exposed to lower doses 10. and 10. n/cm2 or higher dose 10. n/cm failed to continue their growth under the higher level of salinity (200 mM) NaCI. The data also revealed that salinity tolerance was positively associated with high accumulation of osmoprotectants i.e., sucrose and proline. (author)

  18. Cereal cystatins delay sprouting and nutrient loss in tubers of potato, Solanum tuberosum

    OpenAIRE

    Munger, Aurélie; Simon, Marie-Aube; Khalf, Moustafa; Goulet, Marie-Claire; Michaud, Dominique

    2015-01-01

    Background Recent studies have reported agronomically useful ectopic effects for recombinant protease inhibitors expressed in leaves of transgenic plants, including improved tolerance to abiotic stress conditions and partial resistance to necrotrophic pathogens. Here we assessed the effects of these proteins on the post-dormancy sprouting of storage organs, using as a model potato tubers expressing cysteine protease inhibitors of the cystatin protein superfamily. Results Sprout emergence and ...

  19. Nitrogenous substances in potato (Solanum tuberosum L.) tubers produced under organic and conventional crop management

    OpenAIRE

    Diviš, Jiří; Jan BÁRTA; Heřmanová, Veronika

    2007-01-01

    The contribution presents data on crude protein and protein, free amino acid and nitrate contents in potato tubers produced under different crop management – conventional and organic. Field trials were carried out with five potato cultivars on two sites of different altitude in 2005. Mean content of crude protein was significantly higher in tubers from organic crop management than in tubers from conventional system (10.92 and 9.76 % in dry matter, respectively). Similar result was observed in...

  20. EFFECT OF THIOUREA ON YIELD AND QUALITY OF POTATO ( SOLANUM TUBEROSUM L.)

    OpenAIRE

    Mani, F.; Bettaieb, T.; Zheni, K.; Doudech, N.; Hannachi, C.

    2013-01-01

    A field experiment was carried out to study the effect of five concentrations (0, 250, 500, 750 and 1000 mM) of thiourea application on mother tubers on yield and quality attributes of harvested potato, variety Spunta in the region of Chott-Mariem in Tunisia. Treated tubers (Ø = 50 mm) were planted in field after breaking dormancy at a spacing of 80 cm x 30 cm according to completely randomized design (CRD) in three replications. Quality attributes of potato tubers especially fresh matter and...

  1. Effect of Thiourea on Yield and Quality of Potato (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Mani, F.

    2013-02-01

    Full Text Available A field experiment was carried out to study the effect of five concentrations (0, 250, 500, 750 and 1000 mM of thiourea application on mother tubers on yield and quality attributes of harvested potato, variety Spunta in the region of Chott-Mariem in Tunisia. Treated tubers (Ш = 50 mm were planted in field after breaking dormancy at a spacing of 80 cm x 30 cm according to completely randomized design (CRD in three replications. Quality attributes of potato tubers especially fresh matter and dry matter of plant, total reducing sugars in leaves, tuber yield, number and diameter of tubers per plant and number of sprouts per tuber was measured. Soluble proteins and starch content in potato tubers were also quantified.In general we noted that application of thiourea showed significant influence on yield and on quality of tubers comparing to control. Maximum tuber yield per plant, maximum number of tubers per plant and maximum starch content was recorded with 250 mM of thiourea. Moreover, highest dry matter of potato plant was found also at this concentration. While increasing dose of thiourea thereafter it showed slight significant improvement and do not affect significantly the diameter of tubers comparing to control.

  2. Productivity of ten promising chaucha potato cultivars (Solanum tuberosum, Phureja group) in the Cajamarca region

    OpenAIRE

    Luz P. Rojas Mercado; Juan F. Seminario Cunya

    2014-01-01

    The productivity of ten treatments (chaucha cultivars) with three replications, all from the Cajamarca Region, were evaluated in a Randomized Complete Block Design: Peruanita 3 Mountaineer 2 Limeña, Yellow, Clavelina 2 Red 2 Mulla, Huagalina, yellow and red Chimbina mahuay. Plant height, number of stems, number of tubers, number of commercial tubers, total weight of tubers, weight of commercial tubers, tuber dry matter, dry weight of foliage and harvest index were evaluated. The yield ranged ...

  3. Regeneración de Solanum tuberosum L. variedad pastusa suprema a partir de explantes internodales

    Directory of Open Access Journals (Sweden)

    Chaparro Giraldo Alejandro

    2006-06-01

    Full Text Available Una de las vías posibles para aumentar la eficacia de los métodos de transformación genética mediante Agrobacterium tumefaciens es la optimización de la capacidad de regeneración de las plantas de interés. En la Universidad Nacional de Colombia, Sede Bogotá, el grupo de Ingeniería Genética de Plantas (IGP, ha venido trabajando en la obtención de plantas transgénicas a partir de la variedad de papa Diacol Capiro. Es de interés llevar a cabo este tipo de procedimientos en otras variedades de papa colombiana. La variedad Pastusa Suprema
    es un cultivar obtenido mediante mejoramiento genético convencional, que presenta cualidades superiores a otros cultivares. Es importante desarrollar un sistema eficiente de regeneración en esta variedad, como soporte para ulteriores estudios de transferencia de genes que permitan optimizar aún más su rendimiento. En este trabajo se evaluó el efecto de diferentes combinaciones hormonales adicionadas a un medio básico compuesto de sales Murashige y Skogg (1962, sobre el comportamiento callogénico y regenerativo de la variedad
    de Pastusa Suprema. Se realizó un diseño experimental factorial, en arreglo completamente al azar. Los factores corresponden a las hormonas ácido naftalénacetico (ANA, ácido giberélico (AG3 y ZR (Zeatina Ribósido, y los niveles son las concentraciones por factor ZR: 2,0 mg/L y 3,0 mg/L; ANA 0,0 mg/L, 0,02 mg/L, y 0,2 mg/L; y AG3: 0,02 mg/L, 1,0 mg/L. Los tratamientos probados corresponden a todas las combinaciones posibles entre los niveles de hormona evaluados, dando lugar a un total de doce tratamientos. La eficiencia del medio de cultivo se evaluó con base en las variables: porcentaje de inducción de callos, porcentaje de regeneración, y número de regenerantes por explante. El análisis de los datos se realizó con el software estadístico
    SAS versión 8.0. La presencia de ANA demostró ser esencial en la respuesta callogénica y regenerativa de los explantes. Los tratamientos carentes de esta hormona mostraron porcentajes de inducción de callo y regeneración muy bajos (16% +/- 11,3 y 15% +/- 11,7 respectivamente. Los tratamientos con una concentración elevada de ANA (0,2 mg/L, formaron callo en un 100%, pero no alcanzaron un porcentaje de regeneración
    adecuado (26% +/- 16. Los tratamientos en los que se utilizó una concentración baja de ANA (0,02 mg/L, fueron eficientes tanto en la formación de callo, como en la regeneración (86,0 +/- 13 y 70 +/- 22%. El nivel de zeatina ribósido y ácido giberélico utilizados en el medio, demostraron también ser estadísticamente significativos en el comportamiento callogénico y regenerativo. Los ensayos realizados demuestran la importancia de la combinación de los tres tipos hormonales en la inducción de callo, y subsecuente regeneración; probablemente, su interacción a nivel fisiológico crea las condiciones necesarias para que el tejido se desdiferencie y exprese su totipotencialidad. Comparando los resultados obtenidos en los 12 tratamientos para cada una de las variables estudiadas se puede concluir que la adición de 3 mg/L de zeatina ribósido, 0,02 mg/L de ácido naftalénacetico y 1,0 mg/L de ácido gibérelico en el medio de cultivo, constituye una formulación hormonal adecuada para inducir el proceso de organogénesis indirecta sobre la variedad de papa Pastusa Suprema, obteniendo porcentajes de regeneración de 92,9 % +/- 4,9 y un número medio de regenerantes por explante de 6,37 +/- 1,03 en un tiempo total de ocho semanas.

  4. Potencialidade de processamento industrial de cultivares de batatas

    Directory of Open Access Journals (Sweden)

    Émerson Loli Garcia

    2015-10-01

    Full Text Available A industrialização da batata tem sido limitada no Brasil, devido à falta de matéria-prima adequada, sendo a demanda suprida pelas importações de produtos processados. Oito cultivares de batata, adaptadas às condições da região serrana de Minas Gerais, safra 2011, foram avaliadas quanto a sua composição centesimal, cor e conteúdo de amilose. Os resultados obtidos mostraram que as cultivares 'Marcy' e 'Colorado' apresentam características importantes para o cozimento. As cultivares 'Marlen' e 'Pirassu' apresentam atributos requeridos para o processamento na forma de chips e/ou palha e a cultivar 'BRS Ana' para a forma de palitos pré-fritos.

  5. Anti-ulcer activity of Ipomoea batatas tubers (sweet potato)

    OpenAIRE

    Vandana Panda; Madhav Sonkamble

    2012-01-01

    Background: Peptic ulcers occur in that part of the gastrointestinal tract which is exposed to gastric acid and pepsin, i.e., the stomach and duodenum. Gastric and duodenal ulcers are common pathologies that may be induced by a variety of factors such as stress, smoking and noxious agents including non-steroidal anti-inflammatory drugs. Ipomoea batatas tubers (sweet potato) contain ample amounts of antioxidants. It has been proven already by many scientific studies that antioxidants have ul...

  6. Aktuelle Funde von "Solanum"-Arten in Sachsen-Anhalt

    OpenAIRE

    Böhme, Frank; Herz, Eckhard; John, Heino

    2013-01-01

    Der Artrang des Rotbeerigen Nachtschattens Solanum alatum MOENCH [Solanum villosum ssp. alatum (MOENCH) EDMONDS; Solanum miniatum BERNH.] und des Gelbbeerigen Nachtschattens Solanum villosum MILL. s. str. [Solanum villosum ssp. villosum; Solanum luteum MILL.] wird bis heute je nach Autor kontrovers angegeben. So vereinigen WISSKIRCHEN & HAEUPLER (1998: 480), HAWKES & EDMONDS (1972: 197-198) sowie EDMONDS & CHWEYA (1997) die beiden Sippen unter Solanum villosum und unterscheiden sie als Untera...

  7. Pneumonia intersticial em bovinos associada à ingestão de batata-doce (Ipomoea batatas mofada Interstitial pneumonia in cattle fed moldy sweet potatoes (Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Rafael A. Fighera

    2003-12-01

    Full Text Available Uma doença respiratória foi diagnosticada em cinco dentre 23 bovinos (21,7% após terem sido alimentados com batata-doce (Ipomoea batatas mofada em uma pequena propriedade rural em São Vicente do Sul, Rio Grande do Sul, Brasil. Três dos cinco bovinos afetados morreram espontaneamente, e o quarto foi sacrificado para necropsia quando mostrava sinais clínicos respiratórios avançados. A manifestação clínica iniciara cerca de 24 horas após a ingestão das batatas-doces e a evolução clínica foi de 1 a 4 dias. Os sinais clínicos incluíam dispnéia (respiração laboriosa e abdominal, taquipnéia, pescoço estendido com cabeça baixa e dilatação ritmada das narinas. Dois bovinos foram necropsiados. Os achados de necropsia incluíam pulmões distendidos, pálidos e de consistência borrachenta, que não colapsavam quando o tórax era aberto; enfisema e edema acentuados eram evidentes no pulmão. Os linfonodos e o baço apresentavam alterações características de hiperplasia linfóide. Histologica-mente, as lesões eram típicas de pneumonia intersticial. Os septos alveolares estavam espessados por fibroblastos e células inflamatórias, havia hipertrofia e hiperplasia de pneumócitos tipo II; os septos interlobulares estavam distendidos por edema e enfisema. A cultura de amostras das batatas-doces mofadas produziu Fusarium solani e F. oxysporum.Cases of respiratory disease were diagnosed in five out of 23 cattle (21.7% after they were fed moldy damaged sweet potatoes (Ipomoea batatas on a small farm in the county of São Vicente do Sul, state of Rio Grande do Sul, Brazil. Of those five cattle, three died spontaneously and another one was euthanatized for necropsy while showing advanced respiratory clinical signs. The disease manifested itself approximately 24 hours after the ingestion of the sweet potatoes and lasted from 1 to 4 days. Clinical signs included dyspnea (labored breathing and abdominal respiration, tachypnea, extended

  8. Solanum nigrum Linn.- A review

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    T S Mohamed Saleem

    2009-01-01

    Full Text Available Solanum nigrum Linn. (Solanaceae commonly known as ′Black nightshade′ that have been extensively used in traditional medicine in India and other parts of world to cure liver disorders, chronic skin ailments (psoriasis and ringworm, inflammatory conditions, painful periods, fevers, diarrhoea , eye diseases, hydrophobia, etc. It has been found that Solanum nigrum contains the substances, such as total alkaloid, steroid alkaloid, steroidal saponins and glycoprotein, exhibiting anti-tumor activity. In Indian traditional medicine, the plant is used as a hepatoprotective agent. In this review, we have explored the phyto-pharmacological properties of the Solanum nigrum plant and compiled its vast pharmacological applications to comprehend and synthesize the subject of its potential image of multipurpose medicinal agent.

  9. Endogenous pararetroviral sequences in tomato (Solanum lycopersicum and related species

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    Machado Margit Laimer

    2007-05-01

    Full Text Available Abstract Background Endogenous pararetroviral sequences (EPRVs are a recently discovered class of repetitive sequences that is broadly distributed in the plant kingdom. The potential contribution of EPRVs to plant pathogenicity or, conversely, to virus resistance is just beginning to be explored. Some members of the family Solanaceae are particularly rich in EPRVs. In previous work, EPRVs have been characterized molecularly in various species of Nicotiana including N.tabacum (tobacco and Solanum tuberosum (potato. Here we describe a family of EPRVs in cultivated tomato (Solanum lycopersicum L. and a wild relative (S.habrochaites. Results Molecular cloning and DNA sequence analysis revealed that tomato EPRVs (named LycEPRVs are most closely related to those in tobacco. The sequence similarity of LycEPRVs in S.lycopersicum and S.habrochaites indicates they are potentially derived from the same pararetrovirus. DNA blot analysis revealed a similar genomic organization in the two species, but also some independent excision or insertion events after species separation, or flanking sequence divergence. LycEPRVs share with the tobacco elements a disrupted genomic structure and frequent association with retrotransposons. Fluorescence in situ hybridization revealed that copies of LycEPRV are dispersed on all chromosomes in predominantly heterochromatic regions. Methylation of LycEPRVs was detected in CHG and asymmetric CHH nucleotide groups. Although normally quiescent EPRVs can be reactivated and produce symptoms of infection in some Nicotiana interspecific hybrids, a similar pathogenicity of LycEPRVs could not be demonstrated in Solanum L. section Lycopersicon [Mill.] hybrids. Even in healthy plants, however, transcripts derived from multiple LycEPRV loci and short RNAs complementary to LycEPRVs were detected and were elevated upon infection with heterologous viruses encoding suppressors of PTGS. Conclusion The analysis of LycEPRVs provides further

  10. Sweet potato (Ipomoea batatas L. LAM.) breeding through tissue culture

    International Nuclear Information System (INIS)

    Somatic embryogenesis was induced on MS medium containing NAA from apical meristems of aseptically growing sweet potato (Ipomoea batatas L. Lam.). It was observed 4 weeks (in earliest cultivar) after the explants were placed on callus induction media, MS containing 1 - 5 gm/INAA, 1 - 10 mg/l GA3, 3% sucrose and 0.2% Gellan Gum. Embryogenesis was observed in each cultivar tested, but the frequency of embryogenesis depended on genotype. Somatic embryogenesis was influenced by the amount of nitrogen supply in the callus induction media. The lower amount of nitrogen seemed to promote somatic embryogenesis. (author). 7 refs, 7 tabs

  11. Wound healing activity of Ipomoea batatas tubers (sweet potato

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    Madhav Sonkamble

    2011-10-01

    Full Text Available Background: Ipomoea batatas (L. Lam. from the family Convolvulaceae is the world’s sixth largest food crop. The tubers of Ipomoea batatas commonly known as sweet potato are consumed as a vegetable globally. The tubers contain high levels of polyphenols such as anthocyanins and phenolic acids and vitamins A, B and C, which impart a potent antioxidant activity that can translate well to show wound healing effects. To check their effects on wound healing, the peels and peel bandage were tested on various injury models in rats in the present study.Methods: The methanolic extracts of the peels and peel bandage of Ipomoea batatas tubers (sweet potato were screened for wound healing by excision and incision wound models on Wistar rats. Three types of gel formulations were prepared, viz., gel containing 3.0% (w/w peel extract, gel containing 6.0% (w/w peel extract and gel containing 10% (w/w peel extract. Betadine (5% w/w povidone iodine cream was used as a reference standard. In the incision wound model, Tensile strength of the skin was measured. Epithelization time, wound contraction, hydroxyproline content of the scab, and ascorbic acid and malondialdehyde content of the plasma were determined in the excision wound model.Results: In the incision wound model, high tensile strength of the wounded skin was observed in animals treated with the peel extract gels and the peel bandage when compared with wounded control animals. The increase in tensile strength indicates the promotion of collagen fibers and that the disrupted wound surfaces are being firmly knit by collagen. In the excision wound model, significant wound closure was observed on the 4th day in rats treated with all three gel formulations when compared with the wounded control rats. A significant increase inFunctional Foods in Health and Disease 2011; 10:403-415hydroxyproline and ascorbic acid content in the gel-treated animals and a significant decrease in malondialdehyde content in the

  12. MORPHOPHYSIOLOGICAL CHANGES OF IPOMOEA BATATAS (L. LAM. DURING DROUGHT STRESS

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    La Rosa, R.

    2008-01-01

    Full Text Available One of the greatest problems in the world is desertification so we must to know physiological responses and morphological adaptations from species with tolerance to drought stress. Sweet potato (Ipomoea batatas Lam. is one of the most important crops of the world, especially because of its great tolerance to drought stress. Hence, we decided to know what morphophysiological and metabolic responses has I. batatas under drought stress. So, we got cuttings of variety INA-100, from INIA Huaral, and were planted in 22 recipients of 4 L with substrate consisting in a mixing of sand and humus (3:1. Half of them were irrigated monthly and other half only receives water to establish plants and no more after that. We measured transpiration, histological changes in leaves and metabolic changes at protein and starch level. We confirmed transpiration is not affected for air humidity or substrate humidity. But, the effect of drought can be better observed in foliage growth, therefore in histological changes in leaves. No significance in concentration of proteins but significance in carbohydrates concentration shows an osmotic regulation in order to maintain stomata open and also an increase in photosynthetic activity in stressed plants.

  13. Ipomoea batatas and Agarics blazei ameliorate diabetic disorders with therapeutic antioxidant potential in streptozotocin-induced diabetic rats

    OpenAIRE

    Niwa, Atsuko; Tajiri, Takashi; Higashino, Hideaki

    2011-01-01

    Ipomoea batatas, Agaricus blazei and Smallanthus sonchifolius are known to favorably influence diabetes mellitus. To clarify their antidiabetic efficacy and hypoglycemic mechanisms, we treated streptozotocin-induced diabetic rats with daily oral feeding of powdered Ipomoea batatas (5 g kg−1 d−1), Agaricus blazei (1 g kg−1 d−1) or Smallanthus sonchifolius (4 g kg−1 d−1) for 2 months. Treatments with Ipomoea batatas or Agaricus blazei, but not Smallanthus sonchifolius, significantly suppressed ...

  14. Transcriptome analysis of Solanum melongena L. (eggplant) fruit to identify putative allergens and their epitopes.

    Science.gov (United States)

    Ramesh, Kumar Ramagoni; Hemalatha, R; Vijayendra, Chary Anchoju; Arshi, Uz Zaman Syed; Dushyant, Singh Baghel; Dinesh, Kumar Bharadwaj

    2016-01-15

    Eggplant is the third most important Solanaceae crop after tomato and potato, particularly in India and China. A transcriptome analysis of eggplant's fruit was performed to study genes involved in medicinal importance and allergies. Illumina HiSeq 2000 system generated 89,763,638 raw reads (~18 Gb) from eggplant. High quality reads (59,039,694) obtained after trimming process, were assembled into a total of 149,224 non redundant set of transcripts. Out of 80,482 annotated sequences of eggplant fruit (BLASTx results against nr-green plant database), 40,752 transcripts showed significant similarity with predicted proteins of Solanum tuberosum (51%) followed by Solanum lycopersicum (34%) and other sequenced plant genomes. With BLASTx top hit analysis against existing allergens, a total of 1986 homologous allergen sequences were found, which had >37% similarity with 48 different allergens existing in the database. From the 48 putative allergens, 526 B-cell linear epitopes were identified using BepiPred linear epitope prediction tool. Transcript sequences generated from this study can be used to map epitopes of monoclonal antibodies and polyclonal sera from patients. With the support of this whole transcriptome catalogue of eggplant fruit, complete list of genes can be predicted based on which secondary structures of proteins may be modeled. PMID:26424595

  15. Yam (Dioscorea batatas) tuber mucilage exhibited antioxidant activities in vitro.

    Science.gov (United States)

    Hou, Wen-Chi; Hsu, Feng-Lin; Lee, Mei-Hsien

    2002-12-01

    The yam (Dioscorea batatas Decne) tuber mucilage (YTM) was extracted and partially purified by SDS and heating treatments. This purified YTM exhibited antioxidant activities in a series of in vitro tests, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical (half-inhibition concentration, IC 50, was 0.86 mg/mL) and hydroxyl radical (IC 50 was 22 microg/mL) scavenging activity assays, reducing power test, anti-lipid peroxidation and anti-human low density lipoprotein peroxidation tests (IC 50 was 145.46 microg/mL) using butylated hydroxytoluene (BHT), reduced glutathione, or ascorbic acid for comparisons. With electron paramagnetic resonance (EPR) spectrometry for DPPH radical detection, the intensities of the EPR signals were decreased by the increased amounts of YTM added (IC 50 was 1.62 mg/mL). These results suggest that mucilage of yam tuber might play roles as antiradicals and antioxidants. PMID:12494332

  16. New species of Solanum (Solanaceae) from Peru and Ecuador

    OpenAIRE

    Sandra Knapp

    2010-01-01

    Abstract Three new species of “non-spiny" Solanum are described from Peru and Ecuador, and a revised description for Solanum verecundum M. Nee is presented. Solanum kulliwaita S. Knapp, sp. nov. (Dulcamaroid clade) is endemic to the Department of Cuzco in southern Peru, and is most similar to the recently described Solanum sanchez-vegae S. Knapp of northern Peru. Solanum dillonii S. Knapp, sp. nov. (Brevantherum clade) is found in southern Ecuador and northern Peru in the Amotape-Huancabamba ...

  17. Utilizing wild relative ((Solanum viarum) as resistant source to shoot and fruit borer in brinjal (Solanum melongena Linn.)

    OpenAIRE

    L.Pugalendhi, D.Veeraragavathatham, S.Natarjan and S. Praneetha

    2010-01-01

    Brinjal (Solanum melongena Linn.) has very important place in Indian curries and also in China, Japan and Southern Europianrecipies. High yield combined with good quality as well as resistance to shoot and fruit borer in a brinjal variety is the ultimateaim in the most of the brinjal breeding programme. Solanum viarum is closely related to Solanum melongena and both are crosscompatible. Therefore hybridization was undertaken in brinjal (Solanum melongena L.) with Solanum viarum to transfer th...

  18. Fenoles, peroxidasa y fenilalanina amonio-lyasa: su relación con la resistencia genética de clones de papa (solamun tuberosum l.) contra el tizón tardío (phytophthora infestans mont de bary)

    OpenAIRE

    Héctor Lozoya Saldaña; María Teresa Colinas León; Rodolfo Rivera Hinojosa

    2007-01-01

    La resistencia horizontal de las plantas a las enfermedades involucra la activación de varios genes de defensa. Para comprobar este tipo de mecanismos se cuantificaron actividades enzimáticas relacionadas con diversos niveles de resistencia genética en genotipos de papa (Solanum tuberosum L.) contra el tizón tardío (Phytophthora infestans Mont. de Bary), expuestos a infección natural en el valle de Toluca, con y sin protección con fungicidas. Hubo respuesta diferencial en presencia y activida...

  19. Monitoramento da população de Phytophthora infestans na região da Zona da Mata de Minas Gerais de 1998 a 2000 Monitoring Phytophthora infestans population in the Zona da Mata region of Minas Gerais, Brazil, from 1998 to 2000

    OpenAIRE

    AILTON REIS; Nelson D. Suassuna; Alfenas, Acelino C; EDUARDO S. G. MIZUBUTI

    2002-01-01

    Foram caracterizados 212 isolados de Phytophthora infestans obtidos de 51 lavouras de tomate (Lycopersicon esculentum)e batata (Solanum tuberosum), em sete municípios da Zona da Mata, MG. Todos os isolados tiveram o grupo de compatibilidade determinado; 96 isolados foram caracterizados para a isoenzima glucose 6-fosfato-isomerase (Gpi); 71 isolados foram analisados quanto à resistência ao metalaxyl; e determinou-se o espectro de virulência de 46 isolados. Todos os 212 isolados testados foram ...

  20. Monitoramento da população de Phytophthora infestans na região da Zona da Mata de Minas Gerais de 1998 a 2000

    OpenAIRE

    REIS AILTON; SUASSUNA NELSON D.; ALFENAS ACELINO C.; MIZUBUTI EDUARDO S. G.

    2002-01-01

    Foram caracterizados 212 isolados de Phytophthora infestans obtidos de 51 lavouras de tomate (Lycopersicon esculentum)e batata (Solanum tuberosum), em sete municípios da Zona da Mata, MG. Todos os isolados tiveram o grupo de compatibilidade determinado; 96 isolados foram caracterizados para a isoenzima glucose 6-fosfato-isomerase (Gpi); 71 isolados foram analisados quanto à resistência ao metalaxyl; e determinou-se o espectro de virulência de 46 isolados. Todos os 212 isolados testados foram ...

  1. Biocatalytic reductions by plant tissue - Green alternative to alcohol production

    OpenAIRE

    Gašo-Sokač, Dajana; Nujić, Marija; Bušić, Valentina; Habuda-Stanić, Mirna

    2014-01-01

    The use of biocatalysts for the industrial synthesis of chemicals has been attracting much attention as an environmental friendly synthetic method. Various plants, such as apple (Malus pumila), carrot (Daucus carota), cucumber (Cucumis sativus), onion (Allium cepa), potato (Solanum tuberosum), radish (Raphanus sativus) and sweet potato (Ipomoea batatas) were used as biocatalysts. Enzymes that plants produce are able to perform reactions under mild conditions (pH and temperature), with remarka...

  2. AFLP analysis reveals a lack of phylogenetic structure within Solanum section Petota

    Directory of Open Access Journals (Sweden)

    Vleeshouwers Vivianne GAA

    2008-05-01

    Full Text Available Abstract Background The secondary genepool of our modern cultivated potato (Solanum tuberosum L. consists of a large number of tuber-bearing wild Solanum species under Solanum section Petota. One of the major taxonomic problems in section Petota is that the series classification (as put forward by Hawkes is problematic and the boundaries of some series are unclear. In addition, the classification has received only partial cladistic support in all molecular studies carried out to date. The aim of the present study is to describe the structure present in section Petota. When possible, at least 5 accessions from each available species and 5 individual plants per accession (totally approx. 5000 plants were genotyped using over 200 AFLP markers. This resulted in the largest dataset ever constructed for Solanum section Petota. The data obtained are used to evaluate the 21 series hypothesis put forward by Hawkes and the 4 clade hypothesis of Spooner and co-workers. Results We constructed a NJ tree for 4929 genotypes. For the other analyses, due to practical reasons, a condensed dataset was created consisting of one representative genotype from each available accession. We show a NJ jackknife and a MP jackknife tree. A large part of both trees consists of a polytomy. Some structure is still visible in both trees, supported by jackknife values above 69. We use these branches with >69 jackknife support in the NJ jackknife tree as a basis for informal species groups. The informal species groups recognized are: Mexican diploids, Acaulia, Iopetala, Longipedicellata, polyploid Conicibaccata, diploid Conicibaccata, Circaeifolia, diploid Piurana and tetraploid Piurana. Conclusion Most of the series that Hawkes and his predecessors designated can not be accepted as natural groups, based on our study. Neither do we find proof for the 4 clades proposed by Spooner and co-workers. A few species groups have high support and their inner structure displays also

  3. Penggunaan sari buah pepino (Solanum muricatum Aiton.)

    OpenAIRE

    Safrina

    2013-01-01

    Pepino fruit (Solanum muricatum Aiton.) is one of natural material which high of vitamin B complex, vitamin A , and vitamin C, it’s important to ward free radical. Besides vitamin, pepino fruit also has protein and lipid. Based on the contains of pepino fruit, research has done on pepino extract (Solanum muricatum Aiton.) as a moisturizer natural skin. Concentration of pepino fruit extract used were 2%, 4%, 6%, 8%, 10%, 12% and them compared with blanco and gycerine 2%. Some test have...

  4. A new tetraploid species of Solanum section Solanum (Solanaceae) from Tanzania

    NARCIS (Netherlands)

    Manoko, M.L.K.; Weerden, van der G.M.; Berg, van den R.G.; Mariani, C.

    2012-01-01

    Solanum umalilaense Manoko sp. nov. (Solanaceae) is described from the Umalila area, in the southern highlands of Tanzania. Its novelty is supported with both morphological and AFLP data. Phenetic and phylogenetic analyses place Solanum umalilaense as a unique and well-supported taxon among tetraplo

  5. Oil and fatty acids in eggplant (Solanum melongena L.) and some related and unrelated Solanum Spp.

    Science.gov (United States)

    The seed oil content of 305 genebank accessions of eggplant (Solanum melongena L. ), six related specied (S. aethiopicum L., S. incanum L., S. anaguivi Lam., S linnaeanum Hepper & P.M.L. Jaeger, S. litchensteinii L., and S. macrocarpon L.) and 25 additional Solanum species, was determined by NMR. Eg...

  6. Distribución Diferencial de Bacterias con Potencial Biocontrolador de Spongospora subterranea en Plantas de Papa (Solanum tuberosum cv. Diacol Capiro) Differential Distrubution of Candidadate Biocontrol Bacteria against Spongospora subterranea in Potato Plants (Solanum tuberosum cv. Diacol Capiro)

    OpenAIRE

    Juliana Soler Arango; Luisa Fernanda Posada Uribe; Juan Carlos Pérez Naranjo

    2012-01-01

    Resumen. La búsqueda de microorganismos promisorios para biocontrol de patógenos de plantas en el suelo con frecuencia se ha orientado a la detección de productores de hormonas y enzimas líticas, condiciones asociadas a su capacidad para promover crecimiento vegetal. Sin embargo, el suelo es altamente variable en escalas pequeñas y se conoce poco sobre la distribución espacial de microorganismos que expresan esas funciones. Este estudio determinó la capacidad de producción de indoles totales ...

  7. Construcción de un Inmunosensor Amperométrico utilizando Apirasa de Solanum tuberosum para la Detección de Esquistosomiasis Construction of an Amperometric Immunosensor using Solanum tuberosum potato Apyrase for the Detection of Schistosomiasis

    Directory of Open Access Journals (Sweden)

    Ninoska I Bojorge Ramírez

    2009-01-01

    Full Text Available Este trabajo presenta el desarrollo de un inmunosensor amperométrico composito a base de una proteína de origen vegetal para la determinación de antí-anticuerpo de S. mansoni. El inmunosensor consiste en una matriz electródica rígida construida con grafito y resina epoxi en la que se inmoviliza covalentemente la Apirasa. La estrategia de inmovilización se basa en silanización de la matriz compósita con 3-aminopropilsilano (3-APTES y su activación mediante glutaraldehído. La inmovilización fue monitoreada mediante voltaamperometría cíclica en Fe(CN6(4-/Fe(CN6³, obteniéndose una respuesta cuasi-reversible del inmunosensor y muy estable en el tiempo. La inmovilización también fue caracterizada mediante ángulos de contactos y microscopía electrónica de barrido. Estas cualidades son aprovechables para la determinación clínica de esquistosomiasis dada la rapidez y sensibilidad de respuesta.This paper presents the development of an amperometric composites inmunosensor based on a vegetable protein for the determination of anti-Ac S. mansoni. The inmunosensor consists of a rigid matrix built with graphite and epoxy resin in which immobilizes covalently the Apyrase. The strategy of immobilization is based in silanization of the matrix composites with 3-Aminopropyltriethoxysilane (3-APTES and its activation by glutaraldehyde. The detection was monitored by cyclic voltammetry Fe(CN6(4-/ Fe(CN6³, obtaining a response quasi-reversible of the immunosensor and very stable over time. The immobilization was also characterized by angles contacts and scanning electron microscopy. These qualities are useful for determination clinical of schistosomiasis given the speed of response and sensitivity.

  8. Índices agronómicos para determinar tolerancia a sequía en variedades de papa (Solanum tuberosum L./Agronomic index to determine tolerance to drought in varieties of potato (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Alfredo Morales Rodríguez

    2016-01-01

    Full Text Available RESUMEN Durante estas últimas temporadas, la sequía ha causado pérdidas del 50 % de la producción de papa en varios países de Centro América. El conocimiento y la utilización de variedades de papa tolerantes a la sequía son elementos clave para incrementar la producción y el rendimiento bajo las condiciones de estrés. Los experimentos fueron desarrollados en el Instituto de Investigaciones de Viandas Tropicales (INIVIT, durante los meses de diciembre de 2014 a marzo de 2015. Se plantaron 10 parcelas por variedad, a la mitad de ellas (5 se les retiró el riego, a partir de los 50 días después de la plantación por espacio de 20 días (período de estrés por sequía reiniciándose el riego a partir de los 70 días. En el momento de la cosecha se determinó: el Índice de Susceptibilidad a Sequía (SSI, Índice de Tolerancia a Sequía (STI, Índice de Tolerancia (TOL, Media de Productividad (MP e Índice de estabilidad del Rendimiento (YSI. Las variedades Atlas y Maranca poseen los menores valores de SSI, con 0,67 y 0,61 respectivamente, y los mayores valores de STI, con 0,79 y 0,81 respectivamente. La MP más alta registrada, fue en la variedad Atlas, con 19,92 t/ha. Los valores de YSI más altos pertenecen a las variedades Maranca y Atlas, con 81,07 y 79,29 % respectivamente. ABSTRACT During recent seasons, drought has caused losses of 50 % of potato production in several countries in Central America. Knowledge and use of varieties tolerant to drought is a key to increase potato production and yield under stress conditions. The experiments were done in the Research Institute of Tropical Roots and Tubers Crop (INIVIT during the months of December 2014 to March 2015. Ten plots per variety were planted; half of them (five was withdrawn irrigation to after 50 days after planting for 20 days (period of drought stress irrigation restarting after 70 days. At the harvest time it was determined: the Stress Susceptibility Index (SSI, Stress Tolerance Index (STI , Tolerance Index (TOL, Mean Productivity ( MP and Yield Stability Index (YSI . Atlas and Maranca varieties have the lowest values of SSI, with 0,67 and 0,61 respectively, and the highest values of STI, with 0,79 and 0,81 respectively. MP recorded the highest was in the Atlas variety, with 19.92 t/ha. Higher values of YSI belong to Atlas and Maranca varieties, with 81,07 and 79,29 % respectively.

  9. Variations observed in the respiratory activity of potato tubers (Solanum tuberosum L.) after a treatment with gamma radiation; Variaciones observadas en la actividad respiratoria de tuberculos de patata (Solanum tuberosum L.) despues de un tratamiento con radiacion GAMMA

    Energy Technology Data Exchange (ETDEWEB)

    Mazon Matanzo, M. P.; Fernandez Gonzalez, J.

    1976-07-01

    The present work studies the variations in the respiratory activity of irradiated and IPC treated potato tubers during a storage period of five months. By immediate effect of gamma radiation we can observe an increase in the oxygen consumption of the parenchyma in relation with the control tubers, such increase persists even fours months after gamma radiation. The respiratory activity is reduced in the IPC treated tubers. In the tissues cultivated in vitro the respiratory activity increases at the end of the cultivation period, not only in the control tissues but also in the irradiated ones, though this increase is greater in the control tissues. (Author) 15 refs.

  10. New species of Solanum (Solanaceae from Peru and Ecuador

    Directory of Open Access Journals (Sweden)

    Sandra Knapp

    2010-11-01

    Full Text Available Three new species of “non-spiny" Solanum are described from Peru and Ecuador, and a revised description for Solanum verecundum M.Nee is presented. Solanum kulliwaita S.Knapp, sp. nov. (Dulcamaroid clade is endemic to the Department of Cuzco in southern Peru, and is most similar to the recently described Solanum sanchez-vegae S.Knapp of northern Peru. Solanum dillonii S.Knapp, sp. nov. (Brevantherum clade is found in southern Ecuador and northern Peru in the Amotape-Huancabamba phytogeographic zone, and is morphologically similar to the widespread Solanum riparium Ruiz & Pav. Solanum oxapampense S.Knapp, sp. nov., (also of the Brevantherum clade is endemic to the Oxapampa region (Department of Pasco of central Peru, and is similar to and segregated from Solanum verecundum M.Nee of Peru and Ecuador. Complete descriptions, distributions and preliminary conservation assessments of all new species are given.

  11. Caracterización postcosecha y composición química de la batata (Ipomoea batatas (L. Lamb. variedad Topera.

    Directory of Open Access Journals (Sweden)

    Auris Damely García-Méndez

    2016-06-01

    Full Text Available El objetivo de este trabajo fue caracterizar la composición química y calidad postcosecha de la batata variedad Topera proveniente de una producción semi-mecanizada con buenas prácticas agrícolas (BPA. El estudio se realizó en el Laboratorio de Procesamiento Primario de Productos Agrícolas y Bioquímica de Alimentos de la Facultad de Agronomía (FAGRO de la Universidad Central de Venezuela (UCV durante el año 2014. La metodología permitió determinar algunas características físicas, texturales y composición química por los métodos de AOAC (Association of Official Analytical Chemists. Se obtuvieron batatas con un menor coeficiente de variabilidad en el peso (CV: 20,12%, forma (CV: 5,63% y tamaño (CV: 12,80%. Esta respuesta permitió establecer tres categorías de calidad con una baja incidencia de defectos (2,15% y daños físico-mecánicos (30,25%, esta última asociada a la alta resistencia del material (6,25+0,05 kgf/mm. Las batatas presentaron contenidos de azúcares reductores de 9,88%, fibra de 6,19% y proteína de 4,13%; estas características nutricionales sugieren su uso como suplemento alimenticio. La producción semi-mecanizada con la implementación de las técnicas de las BPA, permitió obtener batatas con características de importancia para la agroindustria procesadora de alimentos IV Gama y, a nivel del consumidor, con una calidad física homogénea.

  12. PHYTOCHEMICAL EVALUATION OF THREE SPECIES OF SOLANUM L.

    OpenAIRE

    S. Gnana Sundari; S. Rekha; A. Parvathi

    2013-01-01

    The identification of primary and secondary constituents has become the utmost important tool to know the active principles of various medicinal plants. The present study was aimed at identification and evaluation of certain phytochemicals of therapeutic importance in Solanum torvum, Solanum trilobatum and Solanum xanthocarpum of the genus Solanum L., used in folk and traditional medicine to cure respiratory disorders especially bronchial asthma. The five different aqueous and organic (chloro...

  13. Gamma irradiation effect on the chemical composition and the antioxidant activity of Ipomoea batatas L.

    Science.gov (United States)

    Tahir, D.; Halide, H.; Wahab, A. W.; Kurniawan, D.

    2014-09-01

    The chemical composition and antioxidant activity of Ipomoea batatas L. (sweet potato) were studied by x-ray fluorescence (XRF) spectroscopy, Fourier transform infrared spectroscopy, and by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity. The irradiation treatment was performed by using Cs-137 as a gamma sources in experimental equipment. Treatment by irradiation emerges as a possible conservation technique that has been tested successfully in several food products. The amount of chemical composition was changed and resulting new chemical for absorbed dose 40 mSv. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by DPPH radical scavenging capacity. The antioxidant activity of Ipomoea batatas L. extract was dramatically increased in the non-irradiated sample to the sample irradiated at 40 mSv. These results indicate that gamma irradiation of Ipomoea batatas L. extract can enhance its antioxidant activity through the formation of a new chemical compound. Based on these results, increased antioxidant activity of Ipomoea batatas L. extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

  14. Gamma irradiation effect on the chemical composition and the antioxidant activity of Ipomoea batatas L

    Energy Technology Data Exchange (ETDEWEB)

    Tahir, D., E-mail: dtahir@fmipa.unhas.ac.id; Halide, H., E-mail: dtahir@fmipa.unhas.ac.id; Kurniawan, D. [Department of Physics, Hasanuddin University, Makassar 90245 (Indonesia); Wahab, A. W. [Department of Chemistry, Hasanuddin University, Makassar 90245 (Indonesia)

    2014-09-25

    The chemical composition and antioxidant activity of Ipomoea batatas L. (sweet potato) were studied by x-ray fluorescence (XRF) spectroscopy, Fourier transform infrared spectroscopy, and by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity. The irradiation treatment was performed by using Cs-137 as a gamma sources in experimental equipment. Treatment by irradiation emerges as a possible conservation technique that has been tested successfully in several food products. The amount of chemical composition was changed and resulting new chemical for absorbed dose 40 mSv. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by DPPH radical scavenging capacity. The antioxidant activity of Ipomoea batatas L. extract was dramatically increased in the non-irradiated sample to the sample irradiated at 40 mSv. These results indicate that gamma irradiation of Ipomoea batatas L. extract can enhance its antioxidant activity through the formation of a new chemical compound. Based on these results, increased antioxidant activity of Ipomoea batatas L. extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

  15. Gamma irradiation effect on the chemical composition and the antioxidant activity of Ipomoea batatas L

    International Nuclear Information System (INIS)

    The chemical composition and antioxidant activity of Ipomoea batatas L. (sweet potato) were studied by x-ray fluorescence (XRF) spectroscopy, Fourier transform infrared spectroscopy, and by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity. The irradiation treatment was performed by using Cs-137 as a gamma sources in experimental equipment. Treatment by irradiation emerges as a possible conservation technique that has been tested successfully in several food products. The amount of chemical composition was changed and resulting new chemical for absorbed dose 40 mSv. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by DPPH radical scavenging capacity. The antioxidant activity of Ipomoea batatas L. extract was dramatically increased in the non-irradiated sample to the sample irradiated at 40 mSv. These results indicate that gamma irradiation of Ipomoea batatas L. extract can enhance its antioxidant activity through the formation of a new chemical compound. Based on these results, increased antioxidant activity of Ipomoea batatas L. extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals

  16. Climate Change: Precipitation and Plant Nutrition Interactions on Potato (Solanum tuberosum L.) Yield in North-Eastern Hungary

    Science.gov (United States)

    László Phd, M., ,, Dr.

    2009-04-01

    It is widely well known that annual temperatures over Europe warm at a rate of between 0.1 0C decade-1 and 0.4 0C decade-1. And most of Europe gets wetter in the winter season between +1% and +4% decade-1. In summer there is a strong gradient of change between northern Europe (wetting of up to +2% decade-1) and southern Europe (drying of up to 5% decade-1). The droughts and the floods were experienced at Hungary in the early eighties as well as today. So among the natural catastrophes, drought and flooding caused by over-abundant rainfall cause the greatest problems in field potato production. The crop is demanding indicator plant of climate factors (temperature, rainfall) and soil nitrogen, phosphorus, potassium and magnesium status. This publication gives the results achieved in the period from 1962 to 2001 of a long term small- plot fertilization experiment set up on acidic sandy brown forest soil at Nyírlugos in the Nyírség region in North-Eastern Hungary. Characteristics of the experiment soil were a pH (KCl) 4.5, humus 0.5%, CEC 5-10 mgeq 100g-1 in the ploughed layer. The topsoil was poor in all four macronutrient N, P, K and Mg. The mineral fertilization experiment involved 2 (genotypes: Gülbaba and Aranyalma) x 2 (ploughed depths: 20 and 40 cm) x 16 (fertilizations: N, P, K, Mg) = 64 treatments in 8 replications, giving a total of 512 plots. The gross and net plot sizes were 10x5=50 m2 and 35.5 m2. The experimental designe was split-split-plot. The N levels were 0, 50, 100, 150 kg ha-1 year-1 and the P, K, Mg levels were 48, 150, 30 kg ha-1 year-1 P2O5, K2O, MgO in the form of 25% calcium ammonium nitrate, 18% superphosphate, 40% potassium chloride, and powdered technological magnesium sulphate. The forecrop every second year was rye. The groundwater level was at a depth of 2-3 m. From the 64 treatments, eight replications, altogether 512- experimental plots with 7 treatments and their 16 combinations are summarised of experiment period from 1962 to 1979. The main conclusions were as follows: 1. The experiment years (1962-1963, 1964-1965, 1966-1967, 1968-1969, 1970-1971, 1972-1973, 1974-1975, 1976-1977, 1978-1979) were characterised by frequent extremes of climate. Seven years had an average rainfall, one year had an over rainfall and one year had a very dry by Hungarian traditional and RISSAC-HAS (Márton 2001b) new potato ecological standards. 2. The unfavorable effects of climate anomalies (drought, over-abundance of water in the topsoil) on the yield formation, yield quantity of potato depended decisively on the time of year when they were experienced and the period for which they lasted. 3. Precipitation deficiency (droughts) in the winter could not be counterbalanced by average rainfall during the vegetation period, and its effect on the yield was similar to that of summer drought. 4. Yield was influenced by rainfall to a greater extent than by 0-150 kg ha-1 nitrogen and NP, NK, NPK, NPKMg combinations. 5. Drought and over rainfall negative effects were decreased by increasing N- doses and its combinations of potassium, phosphorous and magnesium from 13 to 32%. 6. It was found the polynomial correlation between rainfall and yield could be observed in the case of N: Y'=380.18-2.95x+0.0056x2, n=72, R2=0.95, NP: Y'=387.19-3.04x+0.0059x2, n=72, R2=0.96, NK: Y'=381.65-2.95x+0.0056x2, n=72, R2=0.95, NPK: Y'=390.87-3.07x+0.0060x2, n=72, R2=0.96 and NPKMg: Y'=390.45-3.06x+0.0059x2, n=72, R2=0.96 nutrition systems. The optimum yields ranges between 17-20 t ha-1 at 280-330 mm of rainfall. Key words: climate change, rainfall, potato, N, NP, NK, NPK, NPKMg, yield Introduction: Climate change was recognized as a serious environmental issue. The build up of greenhouse gases in the atmosphere and the inertia in trends in emissions means that we can expect significant changes for at least the next few decades and probably for the future time. Annual temperatures over Europe warm at a rate of between 0.1 0C decade-1 and 0.4 0C decade-1. And most of Europe gets wetter in the winter season between +1% and +4% decade-1. In

  17. MÉTODOS ALTERNATIVOS PARA LA PRODUCCIÓN DE SEMILLA PREBÁSICA DE PAPA (Solanum tuberosum, L.)

    OpenAIRE

    Y. Quiñones; H. Izquierdo; Martínez, O.; P. Alcántara; Rodríguez, E.

    2004-01-01

    El uso del material de alta como material de partida (semilla original) es un requisito inprescindible para obtener semillas saludables, siendo uno de los principales sistemas utilizados la multiplicación in vitro o micropropagación y las técnicas de multiplicación acelerada, donde se incluyen las técnicas de corte de esquejes como alternativas para reducir los costos de producción de la semilla que se obtienen por vía biotecnológica. Las técnicas de multiplicación acelerada constituyen una d...

  18. Growth and stress response in Arabidopsis thaliana, Nicotiana benthamiana, Glycine max, Solanum tuberosum and Brassica napus cultivated under polychromatic LEDs

    Czech Academy of Sciences Publication Activity Database

    Janda, Martin; Navrátil, Oldřich; Haisel, Daniel; Jindřichová, Barbora; Fousek, Jan; Burketová, Lenka; Čeřovská, Noemi; Moravec, Tomáš

    2015-01-01

    Roč. 11, MAY 3 (2015). ISSN 1746-4811 R&D Projects: GA ČR GA13-26798S; GA ČR(CZ) GAP501/12/1942; GA ČR(CZ) GAP501/12/1761; GA ČR(CZ) GA15-10768S Institutional support: RVO:61389030 Keywords : LED * Fluorescent tubes * Plant physiology Subject RIV: GF - Plant Pathology, Vermin, Weed, Plant Protection Impact factor: 3.102, year: 2014

  19. Crecimiento y desarrollo en patata (Solanum tuberosum L.) con diferentes tratamientos hídricos en sistema pivote

    OpenAIRE

    Costa Camargo, Débora

    2013-01-01

    La agricultura de regadío en regiones áridas y semiáridas, donde los recursos hídricos son la base de su progreso, necesita la búsqueda de un desarrollo sostenible a partir del uso eficiente de los recursos, entre ellos el agua y la energía, para obtener el mínimo impacto ambiental y maximizar el beneficio económico en la agricultura (Fragoso y Marques, 2006; Durão 2008). En España, la patata se considera habitualmente un cultivo hortícola que dinamiza su tejido social, ambiental y económi...

  20. Solanum tuberosum and Lycopersicon esculentum Leaf Extracts and Single Metabolites Affect Development and Reproduction of Drosophila melanogaster.

    Science.gov (United States)

    Ventrella, Emanuela; Adamski, Zbigniew; Chudzińska, Ewa; Miądowicz-Kobielska, Mariola; Marciniak, Paweł; Büyükgüzel, Ender; Büyükgüzel, Kemal; Erdem, Meltem; Falabella, Patrizia; Scrano, Laura; Bufo, Sabino Aurelio

    2016-01-01

    Glycoalkaloids are secondary metabolites commonly found in Solanaceae plants. They have anti-bacterial, anti-fungal and insecticidal activities. In the present study we examine the effects of potato and tomato leaf extracts and their main components, the glycoalkaloids α-solanine, α-chaconine and α-tomatine, on development and reproduction of Drosophila melanogaster wild-type flies at different stages. Parental generation was exposed to five different concentrations of tested substances. The effects were examined also on the next, non-exposed generation. In the first (exposed) generation, addition of each extract reduced the number of organisms reaching the pupal and imaginal stages. Parent insects exposed to extracts and metabolites individually applied showed faster development. However, the effect was weaker in case of single metabolites than in case of exposure to extracts. An increase of developmental rate was also observed in the next, non-exposed generation. The imagoes of both generations exposed to extracts and pure metabolites showed some anomalies in body size and malformations, such as deformed wings and abdomens, smaller black abdominal zone. Our results further support the current idea that Solanaceae can be an impressive source of molecules, which could efficaciously be used in crop protection, as natural extract or in formulation of single pure metabolites in sustainable agriculture. PMID:27213896

  1. Solanum tuberosum and Lycopersicon esculentum Leaf Extracts and Single Metabolites Affect Development and Reproduction of Drosophila melanogaster.

    Directory of Open Access Journals (Sweden)

    Emanuela Ventrella

    Full Text Available Glycoalkaloids are secondary metabolites commonly found in Solanaceae plants. They have anti-bacterial, anti-fungal and insecticidal activities. In the present study we examine the effects of potato and tomato leaf extracts and their main components, the glycoalkaloids α-solanine, α-chaconine and α-tomatine, on development and reproduction of Drosophila melanogaster wild-type flies at different stages. Parental generation was exposed to five different concentrations of tested substances. The effects were examined also on the next, non-exposed generation. In the first (exposed generation, addition of each extract reduced the number of organisms reaching the pupal and imaginal stages. Parent insects exposed to extracts and metabolites individually applied showed faster development. However, the effect was weaker in case of single metabolites than in case of exposure to extracts. An increase of developmental rate was also observed in the next, non-exposed generation. The imagoes of both generations exposed to extracts and pure metabolites showed some anomalies in body size and malformations, such as deformed wings and abdomens, smaller black abdominal zone. Our results further support the current idea that Solanaceae can be an impressive source of molecules, which could efficaciously be used in crop protection, as natural extract or in formulation of single pure metabolites in sustainable agriculture.

  2. Detección de Ralstonia solanacearum en Solanum tuberosum L. en el Estado de Sonora, México

    OpenAIRE

    Ana G. Alvarado-Martínez; Edgar O. Rueda-Puente; Juan F. Ponce-Medina; Leonel Avendaño-Reyes; Jesús Santillano-Cazares; Jesús Borboa-Flores; Luis Hernández-Montiel; Ramón Holguín-Peña

    2013-01-01

    Ralstonia solanacearum (Rs) produce la enfermedad cuarentenaria denominada marchitez bacteriana en papa. México es un país importador de semilla de Estados Unidos de América y Canadá, aspecto significante para provocar una eventual introducción de esta enfermedad en áreas con amplias extensiones de papa. Sonora es una región importante en relación con la producción de este cultivo. Por lo anteriormente expuesto, se realizó la presente investigación, teniendo como objetivos: a) la ...

  3. Pasting, textural and thermal properties of resistant starch prepared from potato (Solanum tuberosum) starch using pullulanase enzyme.

    Science.gov (United States)

    Reddy, Chagam Koteswara; Pramila, S; Haripriya, Sundaramoorthy

    2015-03-01

    Pullulanase enzyme (40 U/g, 10 h) was used for enzymatic hydrolysis of potato starch which was autoclaved (121 °C/30 min), stored under refrigeration (4 °C/24 h) and lyophilized. Comparison of morphological, pasting, textural and thermal properties among native hydrolysed starch (V2) and gelatinized hydrolysed starch (V3) prepared using pullulanase enzyme on potato starch (V1) were studied. The round, elliptical, irregular and oval shape with smooth surface of V1 was replaced with amorphous mass of cohesive structure leading to loss of granular appearance in V2 and V3. The percentage of amylose and resistant starch content of V2 (27.16 %) and (24.16 %); V3 (51.44 %) and (29.35 %) was higher when compared to V1 (22.17 %) and (3.62 %). The swelling power of V1 observed at 60 °C (0.85 %) and 95 °C (8.64 %) were significantly different from V2 at 60 °C (4.97 %) and 95 °C (7.66 %) and that of V3 at 60 °C (5.82 %) and 95 °C (7.5 %). Significance difference in water solubility (7.62 %) and absorption capacity (6.11 %) was noted in V3 when compared with V1 and V2 owing to amylose/amylopectin content. Increase in water solubility and absorption capacity along with decrease in swelling power of V2 and V3 was noted due to hydrolytic and thermal process. RS obtained from hydrolysis showed a reduction in viscosity, indicating the rupture of starch molecules. The viscosity was found to be inversely proportional to the RS content in the sample. The thermal properties of RS increased due to the retrogradation and recrystallization (P < 0.05). PMID:25745229

  4. Discriminative study of a potato (Solanum tuberosum L.) cultivation region by measuring the stable isotope ratios of bio-elements.

    Science.gov (United States)

    Chung, Ill-Min; Kim, Jae-Kwang; Jin, Yong-Ik; Oh, Yong-Taek; Prabakaran, Mayakrishnan; Youn, Kyoung-Jin; Kim, Seung-Hyun

    2016-12-01

    Compared to other foods, the use of common bio-elements to identify the geographical origin of potato remains limited. Thus, this study aimed to verify whether the cultivation regions of raw potato tubers could be determined by the stable isotope composition analysis of bio-elements. δ(13)CVPDB and δ(15)NAIR in potato were influenced by region and cultivar, whereas δ(18)OVSMOW and δ(34)SVCDT were only influenced by region (p<0.0001). A two-dimensional plot of δ(18)OVSMOW and δ(34)SVCDT effectively distinguished between high and low altitude regions, and also reliably discriminated Wanju, Haenam, and Boseong cultivars in low altitude regions. δ(34)SVCDT was the main component that was responsible for the separation of samples in the principal component analysis (eigenvector of -0.6209) and orthogonal projection to latent structure-discriminant analysis (VIP value of 1.0566). In conclusion, this study improves our understanding of how the isotope composition of potato tubers varies with respect to cultivation regions and cultivars. PMID:27374505

  5. Variations observed in the respiratory activity of potato tubers (Solanum tuberosum L.) after a treatment with gamma radiation

    International Nuclear Information System (INIS)

    The present work studies the variations in the respiratory activity of irradiated and IPC treated potato tubers during a storage period of five months. By immediate effect of gamma radiation we can observe an increase in the oxygen consumption of the parenchyma in relation with the control tubers, such increase persists even fours months after gamma radiation. The respiratory activity is reduced in the IPC treated tubers. In the tissues cultivated in vitro the respiratory activity increases at the end of the cultivation period, not only in the control tissues but also in the irradiated ones, though this increase is greater in the control tissues. (Author) 15 refs

  6. Effect of multi-nutrient complex fertilizers on growth and tuber yield of very early potato (Solanum tuberosum L. cultivars

    Directory of Open Access Journals (Sweden)

    Wanda Wadas

    2013-10-01

    Full Text Available The aim of the study was to compare the effect of multi-nutrient complex fertilizers (HydroComplex, Nitrophoska Blue Special and Viking 13 from the nitrophoska group, and Polimag S from the amophoska group and single-nutrient fertilizers on the growth and tuber yield of very early potato cultivars (‘Aster’, ‘Fresco’, ‘Gloria’. The field experiment was carried out on podzolic soil in mid-eastern Poland during 2005–2007. The study showed a greater beneficial effect of HydroComplex and Nitrophoska Blue Special on the growth of very early potato cultivars than that of Viking 13 and Polimag S. The application of HydroComplex and Nitrophoska Blue Special resulted in greater above-ground plant biomass and assimilation leaf area compared with single-nutrient fertilizers; the leaf area index (LAI was higher by 0.28 and 0.32, respectively. The differences were smaller and not statistically confirmed with the use of Polimag S and Viking 13. When the multi-nutrient complex fertilizers were applied, leaf weight ratio (LWR, leaf area ratio (LAR and specific leaf area (SLA were similar to the single-nutrient fertilizers. Of the multi-nutrient complex fertilizers, only Nitrophoska Blue Special resulted in higher tuber yield, on average by 2.40 t × ha-1, compared with the single-nutrient fertilizers. The studied cultivars showed a similar response to applied fertilizers. LAI for ‘Gloria’ was higher than for ‘Aster’ and ‘Fresco’, with smaller LAR found in ‘Gloria’. LWR for ‘Fresco’ was smaller than for ‘Aster’ and ‘Gloria’, with higher SLA found in ‘Fresco’. The tuber yield of ‘Aster” (24.04 t × ha-1 was higher on average by 3 t × ha-1 than for ‘Fresco’ and ‘Gloria’.

  7. The effect of temperature upon the pathogenicity and chemical control of Rhizoctonia solani K. on potato tubers (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    B. Błaszczak

    2015-06-01

    Full Text Available In poe experiment, it was found that Rhizoctonia solani was most pathogenic to potato sprouts at 14.8 and 17.4°C. At a third temperature applied (9.9°C, the pathologenicity was much lower. The higher temperatures decreased the fungicidal activity of Ceresan Nassbeize and Rizokton. The addition to the soil of 1 and 3% of corn straw did not affect the pathogenicity of the fungus.

  8. The effect of temperature upon the pathogenicity and chemical control of Rhizoctonia solani K. on potato tubers (Solanum tuberosum L.)

    OpenAIRE

    B. Błaszczak; Weber, Z.; M. Mańka

    2015-01-01

    In poe experiment, it was found that Rhizoctonia solani was most pathogenic to potato sprouts at 14.8 and 17.4°C. At a third temperature applied (9.9°C), the pathologenicity was much lower. The higher temperatures decreased the fungicidal activity of Ceresan Nassbeize and Rizokton. The addition to the soil of 1 and 3% of corn straw did not affect the pathogenicity of the fungus.

  9. Foliar application of selenite and selenate to potato (Solanum tuberosum): effect of a ligand agent on selenium content of tubers.

    Science.gov (United States)

    Poggi, V; Arcioni, A; Filippini, P; Pifferi, P G

    2000-10-01

    The effect of a foliar spray of selenium on potatoes was investigated for 2 years. Amounts of 0, 50, and 150 g of Se ha(-)(1) were applied both as sodium selenate and as sodium selenite in water, either pure or with the addition of 0.15% of soluble leonardite as a source of humic acids (pH 7). Tuber selenium concentration increased with the application levels, both with sodium selenate and with sodium selenite, when only aqueous solutions were used. When humic acids were added, the tuber selenium level rose more markedly after the application of sodium selenate as compared to the case of the aqueous solutions; however, in the case of sodium selenite, the level showed a large increase only after the application of 50 g of Se ha(-)(1). Kinetics showed that humic acids raised the selenate availability, but no differences were found in the distribution of selenium in the tuber fractions. Foliar application of selenium with humic acids was proven to be a good way to increase the selenium content of potatoes, but the assimilation process of selenium was simpler with selenate than with selenite. PMID:11052729

  10. Yield and Nitrogen Assimilation of Potato Varieties (Solanum tuberosum L.) as Affected by Saline Water Irrigation and Organic Manure

    International Nuclear Information System (INIS)

    The experiment was carried out in lysimeter under controlled greenhouse conditions. Saline water was applied in different levels, i.e. fresh water, 3 and 6 dS/m. Organic manure were applied to soil at rates of 0, 2.6 and 5.2 kg/m2. Basal recommended doses of P and K were applied. Labelled urea (10% a.e.) was applied at rate of 200 kg N/ha. 15N technique was used to evaluate N-uptake and fertilizer efficiency. Comparison held between the two potato varieties indicated that higher reduction in shoot dry weight was recorded with Nicola variety than Spunta one which irrigated with 6 dS/m water salinity level. Addition of 2.6 kg/m2 organic rate induced an increase in N uptake with fresh water and 3 dS/m salinity then tended to decrease with 6 dS/m level as compared to the untreated control. Concerning the nitrogen fertilization, data of 15N analysis showed that, water salinity levels combined with organic addition rates were frequently affected the nitrogen derived from fertilizer and consequently the fertilizer use efficiency. Most of nitrogen was derived from the applied nitrogen fertilizer with maximum accumulation in tuber rather than shoots or roots of both potato varieties. Gradual increase of tuber starch with increasing salinity levels was noticed with addition of 2.6 kg/m2 of organic matter. In general, Spunta variety showed some superiority in tuber starch over those of Nicola variety tuber

  11. Income determinants of Irish potato (Solanum tuberosum L. growers: The case of west Arsi Zone of Oromia Regional State, Ethiopia

    Directory of Open Access Journals (Sweden)

    Abdi Etafa Regassa

    2016-02-01

    Full Text Available Potato is playing a considerable role in enhancing the income of rural households in the study area. This study analyzed determinants of income from potato production and household decision making in potato production and marketing. The study used purposive sampling and simple random sampling to select the district and respondents respectively. Data was collected using semi structured interview and analyzed by STATA software. Multiple regression analysis was used to determine factors influencing income of potato production. Accordingly, seed cost, level of commercialization, number of oxen owned, frequency of extension contact and farming experience affected farmers’ income positively and significantly. Total land owned, and ages of household head are negatively and significantly correlated to potato income. The study implies that to increase the income of potato growers, extension services should be consolidated, accessing quality seed availability through local level seed production system. Extension service should strengthen gender based economic empowerment of woman in potato marketing.

  12. Variation of Growth and Disease Characters Between Clones of Potato (Solanum tuberosum L.)

    DEFF Research Database (Denmark)

    Nielsen, Steen Lykke; Bång, H; Kotkas, K;

    2007-01-01

    The occurrence and magnitude of variation of selected characters in clones derived from meristem tips of four potato cultivars in field trials in three countries was assessed. Recorded were: the number of plants per plot; maturity of plants; skin and flesh colour; tuber deformity; time of emergen...

  13. Two approaches for induction and isolation of starch mutants in potato (Solanum tuberosum L.) : random versus gene targeted mutagenesis

    NARCIS (Netherlands)

    Hoogkamp, T.J.H.

    2001-01-01

    In this thesis two approaches were used to induce structural mutations in potato starch biosynthesis genes in potato. First production of new monoploid amf genotypes through parthenogenesis made it possible to initiate mutation breeding for amfae double mutants. Two amf monoploids were selected whic

  14. Identification of actively filling sucrose sinks. [Solanum tuberosum; Phaseolus lunatus; Manihot esculenta; Liquidambar styraciflua L. ; Carya illinoinensis

    Energy Technology Data Exchange (ETDEWEB)

    Sung, Shijean S.; Xu, Dianpeng; Black C.C. (Univ. of Georgia, Athens (USA))

    1989-04-01

    Certain actively filling plant sucrose sinks such as a seed, a tuber, or a root can be identified by measuring the uridine diphosphate and pyrophosphate-dependent metabolism of sucrose. Sucrolysis in both active and quiescent sucrose sinks was tested and sucrose synthase was found to be the predominant sucrose breakdown activity. Sucrolysis via invertases was low and secondary in both types of sinks. Sucrose synthase activity dropped markedly, greater than fivefold, in quiescent sinks. The test are consistent with the hypothesis that the sucrose filling activity, i.e. the sink strength, of these plant sinks can be measured by testing the uridine diphosphate and pyrophosphate-dependent breakdown of sucrose. Measuring the initial reactions of sucrolysis shows much promise for use in agriculture crop and tree improvement research as a biochemical test for sink strength.

  15. The Effect of Paclobutrazol Application Time and Variety on Growth, Yield, and Quality of Potato (Solanum tuberosum L.)

    OpenAIRE

    Ottilia Mabvongwe; Manenji, Brenda T.; Munyaradzi Gwazane; Misheck Chandiposha

    2016-01-01

    An experiment was set up to investigate the effect of time of paclobutrazol application and variety on growth, yield, and quality of potato in a greenhouse at 34°C (±3) and 21°C (±3) day and night temperatures, respectively, with 60% relative humidity. The experiment was set up as a 2 × 4 factorial design in a CRD with 3 replications. The first factor was potato variety and the levels were BP1 and Diamond. The second factor was paclobutrazol application time and the levels were 28 (Days After...

  16. Effect of gamma-radiation on development, yield and quality of microtubers in vitro in Solanum tuberosum L

    International Nuclear Information System (INIS)

    Explants obtained from in vitro-propagated plantlets of two potato cultivars, Shepody and Atlantic, were treated with five doses of gamma-radiation (0, 2, 4, 6 and 8 Gy) to investigate the stimulating effects of low irradiation on the production and quality of microtubers in vitro. Microtubers of both cultivars treated with gamma-radiation initiated 5 d earlier than in the non-irradiated control. The whole period of microtuberization was prolonged by 10-15 d with 4, 6 and 8 Gy irradiation treatment for cv. Atlantic. Irradiation of the plantlets (4 Gy) led to a significant increase not only in the microtuber number (116.7 and 34.5% over the control) but also in the fresh mass (77.6 and 23.2% in Shepody and Atlantic, respectively). Low dose irradiation (2-4 Gy) increased the starch concentration of microtubers. High doses (6-8 Gy) enhanced ascorbic acid and reducing sugar concentrations. Protein concentrations were increased by 4-6 Gy doses

  17. Variations observed in the respiratory activity of potato tubers (Solanum tuberosum L.) after a treatment with gamma radiation

    International Nuclear Information System (INIS)

    The variations in the respiratory activity of irradiated and IPC treated potato tubers during a storage period of five months have been studied. By immediate effect of gamma radiation, an increase in the oxigen consumption of the parenchyma in relation with the control tubers has been observed. Such increase persits even four months after gamma radiation. The respiratory activity is reduced in the IPC treated tubers. In the tissues cultivated ''in vitro'' the respiratory activity increases at the end of the cultivation period, not only in the control tissues but also in the irradiated ones, though this increase is greater in the control tissues.(author)

  18. The Effect of Paclobutrazol Application Time and Variety on Growth, Yield, and Quality of Potato (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Ottilia Mabvongwe

    2016-01-01

    Full Text Available An experiment was set up to investigate the effect of time of paclobutrazol application and variety on growth, yield, and quality of potato in a greenhouse at 34°C (±3 and 21°C (±3 day and night temperatures, respectively, with 60% relative humidity. The experiment was set up as a 2 × 4 factorial design in a CRD with 3 replications. The first factor was potato variety and the levels were BP1 and Diamond. The second factor was paclobutrazol application time and the levels were 28 (Days After Planting DAP, 35 DAP, and 42 DAP and no paclobutrazol applied (control. Early application of paclobutrazol reduced stem length, number of tubers per plant, and sugar content of potato; furthermore, it increased starch content and yield compared to late application and no paclobutrazol treatments. Early application of paclobutrazol at 28 DAP is recommended in high temperature zones as it increased the yield by 108% and quality of potato.

  19. EVALUACIÓN DEL RENDIMIENTO EN PAPA (Solanum tuberosum , L.) A PARTIR DEL COMPORTAMIENTO DE LAS TEMPERATURAS

    OpenAIRE

    Roberqui Martín Martín; Eduardo Jeréz Mompié

    2015-01-01

    El trabajo se desarrolló en el Instituto Nacional de Ciencias Agrícolas (INCA) con el objetivo de evaluar las respuestas provocadas en el rendimiento, producto de las variaciones de las temperaturas durante el ciclo del cultivo. Se utilizaron plantas de tres variedades de papa Call White, Spunta y Santana cosechadas durante los años 2010, 2011 y 2012, respectivamente. Se evaluó el comportamiento de las temperaturas mínimas, medias y máximas así como la amplitud de estas, además se de...

  20. Greenhouse Trace Gas Fluxes from an Irrigated Sweet Corn (Zea mays L.) - Potato (Solanum tuberosum L.) Rotation

    Science.gov (United States)

    There is a critical need to develop management strategies that improve soil quality through adoption of suitable crop rotations and reduced tillage for carbon sequestration and control of greenhouse gas emissions. In this study emissions of carbon dioxide (CO2), nitrous oxide (N2O), and methane (CH4...

  1. Molecular and biological characterisation of two novel pomo-like viruses associated with potato (Solanum tuberosum) fields in Colombia.

    Science.gov (United States)

    Gil, Jose Fernando; Adams, Ian; Boonham, Neil; Nielsen, Steen Lykke; Nicolaisen, Mogens

    2016-06-01

    Potato is the fourth most important crop worldwide that is used as a staple food, after rice, wheat and maize. The crop can be affected by a large number of pathogens, including fungi, oomycetes, bacteria and viruses. Diseases caused by viruses are among the most important factors contributing to reduced quality and yield of the crop. Potato mop-top virus (genus Pomovirus) induces necrotic flecks in the tuber flesh and skin of potato in temperate countries. Spongospora subterranea is the vector of PMTV. Both the virus and its vector cause disease in potato. In Colombia, PMTV has been detected throughout the country together with a novel pomo-like virus in the centre (Cundinamarca and Boyacá) and south west (Nariño) of the country. We studied the molecular and biological characteristics of this novel virus. Its genome resembles those of members of the genus Pomovirus, and it is closely related to PMTV. It induces mild systemic symptoms in Nicotiana benthamiana (mosaic, branch curling), but no symptoms in N. tabacum, N. debneyi and Chenopodium amaranticolor. The proposed name for the virus is "Colombian potato soil-borne virus" (CPSbV). Additionally, another pomo-like virus was identified in Nariño. This virus induces severe systemic stem declining and mild mosaic in N. benthamiana. The tentative name "soil-borne virus 2" (SbV2) is proposed for this virus. No vectors have been identified for these viruses despite several attempts. This work focused on the characterisation of CPSbV. The risk posed by these viruses if they are introduced into new territories is discussed. PMID:27016929

  2. Folate Profiling in Potato (Solanum tuberosum) Tubers by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry.

    Science.gov (United States)

    Van Daele, Jeroen; Blancquaert, Dieter; Kiekens, Filip; Van Der Straeten, Dominique; Lambert, Willy E; Stove, Christophe P

    2014-03-31

    An ultrahigh-performance liquid chromatography-tandem mass spectrometry method was developed and validated for the profiling of six folate species in potatoes. The calibration curves cover a wide, linear range (the lower and upper limits of quantitation range between 0.22-0.24 and 216.07-242.28 μg/100 g of fresh weight), allowing sensitive determination in small amounts of potato flesh. With a single exception, the acceptance criteria for intra- and interday precision and accuracy were met: for all quality controls, the percent relative standard deviation and the percent bias were lower than 15% (or 20% at the lower limit of quantitation). Application of the method on tubers at different stages of maturation demonstrated the large variability within a single variety: the folate content and polyglutamylation rate varied between 10.35 and 24.01 μg/100 g of fresh weight and between 4.96% and 60.49%, respectively. Additionally, the two-dimensional folate profiling of mature tubers demonstrated an increase in folate from center to peel, combined with a stable species distribution and polyglutamylation rate. PMID:24655154

  3. Genome-wide survey and expression analysis of the amino acid transporter superfamily in potato (Solanum tuberosum L.).

    Science.gov (United States)

    Ma, Haoli; Cao, Xiaoli; Shi, Shandang; Li, Silu; Gao, Junpeng; Ma, Yuling; Zhao, Qin; Chen, Qin

    2016-10-01

    Amino acid transporters (AATs) are integral membrane proteins responsible for the transmembrane transport of amino acids and play important roles in various physiological processes of plants. However, there has not yet been a genome-wide overview of the StAAT gene family to date and only StAAP1 has been previously studied in potato. In this paper, a total of 72 StAATs were identified using a series of bioinformatics searches and classified into 12 subfamilies based on their phylogenetic relationship with known Arabidopsis and rice AATs. Chromosomal localization revealed their distribution on all 12 chromosomes. Nearly one-third of StAAT genes (23 of 72) were derived from gene duplication, among which tandem duplication made the greatest contribution to the expansion of the StAAT family. Motif analysis showed that the same subfamily had similar conserved motifs in both numbers and varieties. Moreover, high-throughput sequencing data was used to analyze the expression patterns of StAAT genes and was verified by quantitative real-time RT-PCR. The expression of StAAT genes exhibited both abundant and tissue-specific expression patterns, which might be connected to their functional roles in long- and short-distance transport. This study provided a comprehensive survey of the StAAT gene family, and could serve as a theoretical foundation for the further functional identification and utilization of family members. PMID:27289266

  4. Effects of Verticillium dahliae infection on stem-end chip defect development in potatoes (Solanum tuberosum L.)

    Science.gov (United States)

    Potato chips are America's favorite snack food with annual retail sales of over $6 billion. Stem-end chip defect, which is characterized by discoloration of the vasculature and surrounding tissues at the tuber stem end portion of chips, is an important tuber quality concern for US chip production. T...

  5. A multi-year survey of stem-end chip defect in chipping potatoes (Solanum tuberosum L.)

    Science.gov (United States)

    One of the most serious tuber quality concerns of US chip potato growers is stem-end chip defect, which is defined as a localized post-fry discoloration in and adjacent to the vasculature on the stem end portion of potato chips. The incidence and severity of stem-end chip defect vary with growing lo...

  6. Effect of Acinetobacter sp on metalaxyl degradation and metabolite profile of potato seedlings (Solanum tuberosum L. alpha variety.

    Directory of Open Access Journals (Sweden)

    Fabiola G Zuno-Floriano

    Full Text Available One of the most serious diseases in potato cultivars is caused by the pathogen Phytophthora infestans, which affects leaves, stems and tubers. Metalaxyl is a fungicide that protects potato plants from Phytophthora infestans. In Mexico, farmers apply metalaxyl 35 times during the cycle of potato production and the last application is typically 15 days before harvest. There are no records related to the presence of metalaxyl in potato tubers in Mexico. In the present study, we evaluated the effect of Acinetobacter sp on metalaxyl degradation in potato seedlings. The effect of bacteria and metalaxyl on the growth of potato seedlings was also evaluated. A metabolite profile analysis was conducted to determine potential molecular biomarkers produced by potato seedlings in the presence of Acinetobacter sp and metalaxyl. Metalaxyl did not affect the growth of potato seedlings. However, Acinetobacter sp strongly affected the growth of inoculated seedlings, as confirmed by plant length and plant fresh weights which were lower in inoculated potato seedlings (40% and 27%, respectively compared to the controls. Acinetobacter sp also affected root formation. Inoculated potato seedlings showed a decrease in root formation compared to the controls. LC-MS/MS analysis of metalaxyl residues in potato seedlings suggests that Acinetobacter sp did not degrade metalaxyl. GC-TOF-MS platform was used in metabolic profiling studies. Statistical data analysis and metabolic pathway analysis allowed suggesting the alteration of metabolic pathways by both Acinetobacter sp infection and metalaxyl treatment. Several hundred metabolites were detected, 137 metabolites were identified and 15 metabolic markers were suggested based on statistical change significance found with PLS-DA analysis. These results are important for better understanding the interactions of putative endophytic bacteria and pesticides on plants and their possible effects on plant metabolism.

  7. The effect of mineral fertilization on fungi colonizing potato (Solanum tuberosum L. tubers after harvest and after storage

    Directory of Open Access Journals (Sweden)

    Bożena Cwalina-Ambroziak

    2012-12-01

    Full Text Available The paper presents the results of a three-year exact experiment conducted in Bałcyny, in which a late potato cultivar, Jasia, was grown. The objective of the study was to determine the effect of different levels of mineral fertilization: A (N 80 kg × ha-1 P 80 kg × ha-1 K120 kg × ha-1 and B (N 120 kg × ha-1 P 144 kg × ha-1 K156 kg × ha-1, and foliar fertilization (Basfoliar 12-4-6, ADOB Mn and Solubor DF on the quantitative and qualitative composition of fungal populations colonizing potato tubers. Fungi were isolated immediately after harvest and after a five-month storage period. After seven days of incubation, fungal colonies were transferred onto agar slants for microscopic identification. Over the entire experimental period, more pathogenic fungi were obtained from potato tubers analyzed after storage (62.9% of the total fungal population after storage than from those analyzed immediately after harvest (39.1%, and the greatest number of fungi was reported in 2004. Rhizoctonia solani was isolated most frequently, followed by Colletotrichum coccodes and Alternaria alternata. Pathogens of the genus Fusarium and the species Helminthosporium solani were not numerous. In the treatment A with soil mineral fertilization with lower NPK rates, larger numbers of pathogenic fungi were noted in 2004 after harvest and after five-month storage, and in 2005 after harvest. At the remaining dates of analysis, pathogens were more frequently isolated from potato tubers in experimental variant B with higher NPK rates. Immediately after harvest, the highest number of pathogenic fungi was isolated in the treatment with foliar application of ADOB Mn and Basfoliar 12-4-6. After five-month storage, pathogens most often colonized potato tubers in experimental variant B with foliar application of Solubor DF, Solubor DF and ADOB Mn, and in experimental variant A with a combination of fertilizers. In the other fertilization variants, including in the control treatment, the population size of pathogenic agents remained at a similar level.

  8. The influence of soil tillage systems and weed control methods on weed infestation of potato (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Krystyna Zarzecka

    2012-12-01

    Full Text Available A field experiment was conducted in the years 2002- 2004 at the Zawady Agricultural Experimental Station (52o06' N; 22o06' E, belonging to the University of Podlasie in Siedlce, Poland. The investigated factors were two soil tillage systems (traditional and reduced and seven methods of weed control in potato canopies with herbicide application. The aim of the study was to determine the influence of tillage systems and weed control methods on the weed species composition and weed density. Tillage systems, weed control methods and atmospheric conditions prevailing in the study years significantly varied weed infestation of potato canopies at the beginning of vegetation and before tuber harvest. The lowest number of weeds, compared to the control treatment, was recorded in the treatments in which chemical and mechanical weed control had been applied. The treatments with the traditional tillage system also showed lower weed infestation than those in which simplifications had been applied.

  9. Effect of Acinetobacter sp on metalaxyl degradation and metabolite profile of potato seedlings (Solanum tuberosum L.) alpha variety.

    Science.gov (United States)

    Zuno-Floriano, Fabiola G; Miller, Marion G; Aldana-Madrid, Maria L; Hengel, Matt J; Gaikwad, Nilesh W; Tolstikov, Vladimir; Contreras-Cortés, Ana G

    2012-01-01

    One of the most serious diseases in potato cultivars is caused by the pathogen Phytophthora infestans, which affects leaves, stems and tubers. Metalaxyl is a fungicide that protects potato plants from Phytophthora infestans. In Mexico, farmers apply metalaxyl 35 times during the cycle of potato production and the last application is typically 15 days before harvest. There are no records related to the presence of metalaxyl in potato tubers in Mexico. In the present study, we evaluated the effect of Acinetobacter sp on metalaxyl degradation in potato seedlings. The effect of bacteria and metalaxyl on the growth of potato seedlings was also evaluated. A metabolite profile analysis was conducted to determine potential molecular biomarkers produced by potato seedlings in the presence of Acinetobacter sp and metalaxyl. Metalaxyl did not affect the growth of potato seedlings. However, Acinetobacter sp strongly affected the growth of inoculated seedlings, as confirmed by plant length and plant fresh weights which were lower in inoculated potato seedlings (40% and 27%, respectively) compared to the controls. Acinetobacter sp also affected root formation. Inoculated potato seedlings showed a decrease in root formation compared to the controls. LC-MS/MS analysis of metalaxyl residues in potato seedlings suggests that Acinetobacter sp did not degrade metalaxyl. GC-TOF-MS platform was used in metabolic profiling studies. Statistical data analysis and metabolic pathway analysis allowed suggesting the alteration of metabolic pathways by both Acinetobacter sp infection and metalaxyl treatment. Several hundred metabolites were detected, 137 metabolites were identified and 15 metabolic markers were suggested based on statistical change significance found with PLS-DA analysis. These results are important for better understanding the interactions of putative endophytic bacteria and pesticides on plants and their possible effects on plant metabolism. PMID:22363586

  10. Effect of Acinetobacter sp on Metalaxyl Degradation and Metabolite Profile of Potato Seedlings (Solanum tuberosum L.) Alpha Variety

    OpenAIRE

    Zuno-Floriano, Fabiola G.; Miller, Marion G.; Aldana-Madrid, Maria L.; Hengel, Matt J.; Gaikwad, Nilesh W.; Vladimir Tolstikov; Ana G Contreras-Cortés

    2012-01-01

    One of the most serious diseases in potato cultivars is caused by the pathogen Phytophthora infestans, which affects leaves, stems and tubers. Metalaxyl is a fungicide that protects potato plants from Phytophthora infestans. In Mexico, farmers apply metalaxyl 35 times during the cycle of potato production and the last application is typically 15 days before harvest. There are no records related to the presence of metalaxyl in potato tubers in Mexico. In the present study, we evaluated the eff...

  11. The effect of mineral fertilization on fungi colonizing potato (Solanum tuberosum L.) tubers after harvest and after storage

    OpenAIRE

    Bożena Cwalina-Ambroziak; Bożena Bogucka

    2012-01-01

    The paper presents the results of a three-year exact experiment conducted in Bałcyny, in which a late potato cultivar, Jasia, was grown. The objective of the study was to determine the effect of different levels of mineral fertilization: A (N 80 kg × ha-1 P 80 kg × ha-1 K120 kg × ha-1) and B (N 120 kg × ha-1 P 144 kg × ha-1 K156 kg × ha-1), and foliar fertilization (Basfoliar 12-4-6, ADOB Mn and Solubor DF) on the quantitative and qualitative composition of fungal populations colonizing potat...

  12. In-season heat stress compromises postharvest quality and low-temperature sweetening resistance in potato (Solanum tuberosum L.).

    Science.gov (United States)

    Zommick, Daniel H; Knowles, Lisa O; Pavek, Mark J; Knowles, N Richard

    2014-06-01

    The effects of soil temperature during tuber development on physiological processes affecting retention of postharvest quality in low-temperature sweetening (LTS) resistant and susceptible potato cultivars were investigated. 'Premier Russet' (LTS resistant), AO02183-2 (LTS resistant) and 'Ranger Russet' (LTS susceptible) tubers were grown at 16 (ambient), 23 and 29 °C during bulking (111-164 DAP) and maturation (151-180 DAP). Bulking at 29 °C virtually eliminated yield despite vigorous vine growth. Tuber specific gravity decreased as soil temperature increased during bulking, but was not affected by temperature during maturation. Bulking at 23 °C and maturation at 29 °C induced higher reducing sugar levels in the proximal (basal) ends of tubers, resulting in non-uniform fry color at harvest, and abolished the LTS-resistant phenotype of 'Premier Russet' tubers. AO02183-2 tubers were more tolerant of heat for retention of LTS resistance. Higher bulking and maturation temperatures also accelerated LTS and loss of process quality of 'Ranger Russet' tubers, consistent with increased invertase and lower invertase inhibitor activities. During LTS, tuber respiration fell rapidly to a minimum as temperature decreased from 9 to 4 °C, followed by an increase to a maximum as tubers acclimated to 4 °C; respiration then declined over the remaining storage period. The magnitude of this cold-induced acclimation response correlated directly with the extent of buildup in sugars over the 24-day LTS period and thus reflected the effects of in-season heat stress on propensity of tubers to sweeten and lose process quality at 4 °C. While morphologically indistinguishable from control tubers, tubers grown at elevated temperature had different basal metabolic (respiration) rates at harvest and during cold acclimation, reduced dormancy during storage, greater increases in sucrose and reducing sugars and associated loss of process quality during LTS, and reduced ability to improve process quality through reconditioning. Breeding for retention of postharvest quality and LTS resistance should consider strategies for incorporating more robust tolerance to in-season heat stress. PMID:24615233

  13. Translucent tissue defect in potato (Solanum tuberosum L.) tubers is associated with oxidative stress accompanying an accelerated aging phenotype.

    Science.gov (United States)

    Zommick, Daniel H; Kumar, G N Mohan; Knowles, Lisa O; Knowles, N Richard

    2013-12-01

    Translucent tissue defect (TTD) is an undesirable postharvest disorder of potato tubers characterized by the development of random pockets of semi-transparent tissue containing high concentrations of reducing sugars. Translucent areas turn dark during frying due to the Maillard reaction. The newly released cultivar, Premier Russet, is highly resistant to low temperature sweetening, but susceptible to TTD. Symptoms appeared as early as 170 days after harvest and worsened with time in storage (4-9 °C, 95 % RH). In addition to higher concentrations of glucose, fructose and sucrose, TTD resulted in lower dry matter, higher specific activities of starch phosphorylase and glc-6-phosphate dehydrogenase, higher protease activity, loss of protein, and increased concentrations of free amino acids (esp. asparagine and glutamine). The mechanism of TTD is unknown; however, the disorder has similarities with the irreversible senescent sweetening that occurs in tubers during long-term storage, where much of the decline in quality is a consequence of progressive increases in oxidative stress with advancing age. The respiration rate of non-TTD 'Premier Russet' tubers was inherently higher (ca. 40 %) than that of 'Russet Burbank' tubers (a non-TTD cultivar). Moreover, translucent tissue from 'Premier Russet' tubers had a 1.9-fold higher respiration rate than the average of non-translucent tissue and tissue from non-TTD tubers. Peroxidation of membrane lipids during TTD development resulted in increased levels of malondialdehyde and likely contributed to a measurable increase in membrane permeability. Superoxide dismutase and catalase activities and the ratio of oxidized to total glutathione were substantially higher in translucent tissue. TTD tubers also contained twofold less ascorbate than non-TTD tubers. TTD appears to be a consequence of oxidative stress associated with accelerated aging of 'Premier Russet' tubers. PMID:24037414

  14. The effect of mangroves amendments to soil on root rot and root knot of potato (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Marium Tariq

    2012-12-01

    Full Text Available Leaves, stem and pneumatophore of Avicennia marina and leaves and stem of Rhizophora mucronata were used as the organic amendments at 0.1, 1 and 5% concentrations in the control of root rot fungi like (Fusarium spp., Rhizoctonia solani and Macrophomina phasoelina and root knot nematode Meloidogyne javanica on potato. In pot experiments, germination of seeds, shoot length, shoot weight, root length, root weight and number of knots were significantly increased when plant parts like leaves, stem and pneumatophore of A. marina and R. mucronata were used at 1 and 5% concentrations. There was a complete suppression in infection of R. solani and M. phaseolina when A. marina and R. mucronata were used at 5% concentration on potato. Maximum inhibition of knots of M. javanica was observed when powder made from mangrove plant parts was used at 1 and 5% concentrations. Powder from all plant parts, like leaves, stem and pneumatophore, was effective in suppression of root infecting fungi and root knot nematode.

  15. Effect of multi-nutrient complex fertilizers on growth and tuber yield of very early potato (Solanum tuberosum L.) cultivars

    OpenAIRE

    Wanda Wadas; Tomasz Dziugieł

    2013-01-01

    The aim of the study was to compare the effect of multi-nutrient complex fertilizers (HydroComplex, Nitrophoska Blue Special and Viking 13 from the nitrophoska group, and Polimag S from the amophoska group) and single-nutrient fertilizers on the growth and tuber yield of very early potato cultivars (‘Aster’, ‘Fresco’, ‘Gloria’). The field experiment was carried out on podzolic soil in mid-eastern Poland during 2005–2007. The study showed a greater beneficial effect of HydroComplex and Nitroph...

  16. PENGARUH KONSENTRASI GIBERELIN TERHADAP PRODUKSI BIBIT KENTANG (SOLANUM TUBEROSUM L. CV. GRANOLA UKURAN M (31 - 60 GRAM

    Directory of Open Access Journals (Sweden)

    Ni Luh Arpiwi

    2007-06-01

    Full Text Available The aim of the research was to increase the production of seed potato size 31 - 60 grams by applying gibberellic acid (GA3. Seeds that passed their dormancy period were dipped in gibberellic acid solutions (10, 15 and 20 mg/L for 15 minutes a day before planting. Control seeds were dipped in water. Seeds were then air dried for 24 hours. Seeds were planted in plots with length, width and height of 150 x 100 x 30 cm respectively with planting space 20 cm and planting depth 15 cm. The design was randomized block with four replications. The results showed that gibberellic acid enhanced shoot emergence. Gibberellic acid increased stem number, tuber number and yield per plant. Tuber number and yield of size M (31 ? 60 g increased, whereas tuber number and yield of size LL decreased drastically. The optimum concentration of gibberellin for these increases was 15 mg/L. Plant height was not influenced by gibberellic acid.

  17. Effect of the gamma radiation on the preservation pf potato tubers (Solanum tuberosum L.) during the storing period

    International Nuclear Information System (INIS)

    The effect of the gamma radiation on the preservation of potato tubers stored during a period of six months is described by evaluating the loss by sprouting, transpiration and infections. The dose of 9 Krad inhibits sprouting with independence of the epoch in wich the treatment is made, on the other hand a dose of 6 Krad inhibits sprouting only if the tubers are irradiated during the resting period. The treatment with IPC gave similar results to those obtained with a dose of 9 Krad. (author)

  18. The changes of the polyphenol content and antioxidant activity in potato tubers (Solanum tuberosum L. due to nitrogen fertilization

    Directory of Open Access Journals (Sweden)

    Diana Hrabovská

    2013-11-01

    Full Text Available 96 Normal 0 false false false CS JA X-NONE Cultivar is one of the most important internal factors affecting polyphenol concentration in the plants. However, influence of the grown locality, climate conditions and way of cultivation belong to important external factors. In our experiment the influence of different nitrogen doses (0 - 40 - 80 - 120 - 160 - 240 kg N.ha-1 applied in the form of Vermikompost on the total polyphenol content and derived total antioxidant activity in cv. Sorento were investigated. While in the 1st - 5th variants the determined polyphenol content in dry mater of potato tubers decreased from 399.2 to 70.40 mg.kg-1, in the 6th variant that was twice higher in comparison to the 5th variants (135.6 mg.kg-1. The statistically significant differences in values of total polyphenol content between variants (polynomial function of 2nd degree were confirmed. The study also confirmed a strong statistical correlation between the content of polyphenols and the content of antioxidant activity has been confirmed (sign. F: 3.24E-10. The highest value of antioxidant activity was observed in the first variant. From the first to the fifth variant (7.62 - 4.84%, the value of antioxidant activity was decreasing and in the sixth variant this value increased to 6.31%.

  19. Development of technique on the induction and selection of in vitro mutant lines(Potato, Solanum tuberosum L.)

    International Nuclear Information System (INIS)

    The radiosensitivity and salt resistance on the single cell and callus of potato, mass production method of plantlet and microtuber of potato by in vitro culture and microtuber formation from the stem irradiated with radiation were investigated to obtain a optimum condition for selection of mutant cell line. (Author)

  20. Cultivation of hitherto-uncultured bacteria belonging to the Verrucomicrobia subdivision 1 from the potato (Solanum tuberosum L.) rhizosphere

    NARCIS (Netherlands)

    da Rocha, Ulisses Nunes; Andreote, Fernando Dini; de Azevedo, Joao Lucio; van Elsas, Jan Dirk; van Overbeek, Leo S.; Andreoti, F.D.

    2010-01-01

    The role of dominant bacterial groups in the plant rhizosphere, e.g., those belonging to the phyla Acidobacteria and Verrucomicrobia, has, so far, not been elucidated, and this is mainly due to the lack of culturable representatives. This study aimed to isolate hitherto-uncultured bacteria from the

  1. Impact of foliar application of seaweed extract on growth, yield and quality of potato (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Muhammad Wasim Haider, Chaudhary Muhammad Ayyub, Muhammad Aslam Pervez, Habat Ullah Asad, Abdul Manan, Syed Ali Raza and Irfan Ashraf

    2012-11-01

    Full Text Available A field trial was carried out in 2010 to investigate the effect of foliar application of seaweed extract “Primo” as an organic biostimulant on potato cv. ‘Sante’. Foliar application of seaweed extract was carried out at different growth stages of the crop (i.e. 30 days; 45 days; 60 days; 30 and 45 days; 30 and 60 days; 45 and 60 days; 30, 45 and 60 days after sowing. Control plants were sprayed with water without seaweed extract. A significant improvement in growth, yield and tuber quality of potato was observed where treatment was applied. The highest tuber yield was recorded with applications of seaweed extract at 30 + 60 days interval after planting. The treatment also improved nitrogen, total soluble solids and protein contents of the potato tubers. The results of the study concluded a positive response of potato plant growth and yield to the foliar application of seaweed extract.

  2. Development of technique on the induction and selection of in vitro mutant lines (Potato, Solanum tuberosum L.)

    International Nuclear Information System (INIS)

    For the development of the technique on the plant tissue culture and application of nuclear technique in the in vitro mutation breeding, present research laid emphasis on the development of techniques of potato tissue culture, and on the induction and selection of radiation mutation. Another culture for haploid induction, optimum radiation dosage for cybrid formation of potato and mutation induction from in vitro cultured microtuber and plantlets were investigated for modelling the technique on the induction and selection of in vitro mutant lines. Inheritance stability of the selected mutants were also studied in field condition. In vitro system of micropropagation and selection of mutation was summarized

  3. Potato Annexin STANN1 Promotes Drought Tolerance and Mitigates Light Stress in Transgenic Solanum tuberosum L. Plants

    Czech Academy of Sciences Publication Activity Database

    Szalonek, M.; Sierpien, B.; Rymaszewski, W.; Gieczewska, K.; Vaňková, Radomíra; Dobrev, Petre; Szczesny, P.; Marczewski, W.; Krusiewicz, D.; Strzelczyk-Zyta, D.; Konopka-Postupolska, D.

    2015-01-01

    Roč. 10, č. 7 (2015), e0132683. E-ISSN 1932-6203 Institutional support: RVO:61389030 Keywords : MEDIATED OXIDATIVE STRESS * VIOLAXANTHIN DE-EPOXIDASE * BRASSICA-JUNCEA ANNEXIN-3 Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.234, year: 2014

  4. Photosynthetic assimilation of 14C into amino acids in potato (Solanum tuberosum) and asparagine in the tubers

    OpenAIRE

    Muttucumaru, Nira; KEYS, ALFRED J.; Parry, Martin A. J.; Powers, Stephen J.; Halford, Nigel G.

    2013-01-01

    Asparagine is the predominant free amino acid in potato tubers and the present study aimed to establish whether it is imported from the leaves or synthesised in situ. Free amino acid concentrations are important quality determinants for potato tubers because they react with reducing sugars at high temperatures in the Maillard reaction. This reaction produces melanoidin pigments and a host of aroma and flavour volatiles, but if free asparagine participates in the final stages, it results in th...

  5. Transgenic potato (Solanum tuberosum) tubers synthesize the full spectrum of inulin molecules naturally occurring in globe artichoke (Cynara scolymus) roots

    OpenAIRE

    Hellwege, Elke M.; Czapla, Sylvia; Jahnke, Anuschka; Willmitzer, Lothar; HEYER, ARND G

    2000-01-01

    The ability to synthesize high molecular weight inulin was transferred to potato plants via constitutive expression of the 1-SST (sucrose:sucrose 1-fructosyltransferase) and the 1-FFT (fructan: fructan 1-fructosyltransferase) genes of globe artichoke (Cynara scolymus). The fructan pattern of tubers from transgenic potato plants represents the full spectrum of inulin molecules present in artichoke roots as shown by high-performance anion exchange chromatography, as well as size exclusion chrom...

  6. High-performance liquid chromatography determination of phenolic acids in potato tubers (Solanum tuberosum) during wound healing

    International Nuclear Information System (INIS)

    The content of phenolic acids formed during wound healing of gamma-irradiated and nonirradiated potato tubers was determined by HPLC. Chlorogenic acid, caffeic acid, p-coumaric acid, and ferulic acid were detected in small quantities in resting whole tubers of irradiated and nonirradiated potatoes. During wound healing their content increased many fold, and in addition, the neo and crypto isomers of chlorogenic acid accumulated in the wound healing tissue. The increased formation of phenolics was accompanied by a parallel rise in phenylalanine ammonia lyase activity. Chlorogenic acid contributed about 56% and together with the neo and crypto isomers accounted for 88% of the phenolics formed. Tubers irradiated to 100 Gy for sprout inhibition showed significantly lower levels of chlorogenic acid and its isomers during the first 8 days of wound healing. The results point to an impairment of wound-induced biosynthesis of phenolics in general and chlorogenic acid and its isomers in particular by irradiation

  7. EVALUACIÓN MORFOAGRONÓMICA DE PROGENIES DE SEMILLA BOTÁNICA DE PAPA (Solanum tuberosum, L. EN CUBA

    Directory of Open Access Journals (Sweden)

    Jorge L. Salomón Díaz

    2014-01-01

    Full Text Available Durante las campañas agrícolas 2006-2007, 20007-2008 y 2008-2009 se sembraron en condiciones semicontroladas (casa de cultivo semillas botánica (SB de papa obtenidas por hibridación y de polinización libre (PL. El objetivo de este trabajo fue evaluar caracteres morfoagronómicos y la uniformidad de los tubérculos. Se encontró variabilidad en las progenies, aunque las progenies híbridas Atlantic x Aninca, 2-13-98 x 1-10-96 y Yara x 9-80-98 mostraron alta uniformidad para un color del tubérculo. Mientras que Atlantic x Aninca y Samila-PL mostraron alta uniformidad para una forma del tubérculo. El vigor, la altura de la planta y rendimiento de tubérculo podrían estar relacionados al efecto de heterosis o vigor hibrido en las progenies híbridas y al efecto depresivo de la endocría en las progenies de PL en grados variables.

  8. EVALUACIÓN DEL RENDIMIENTO EN PAPA (Solanum tuberosum , L. A PARTIR DEL COMPORTAMIENTO DE LAS TEMPERATURAS

    Directory of Open Access Journals (Sweden)

    Roberqui Martín Martín

    2015-01-01

    Full Text Available El trabajo se desarrolló en el Instituto Nacional de Ciencias Agrícolas (INCA con el objetivo de evaluar las respuestas provocadas en el rendimiento, producto de las variaciones de las temperaturas durante el ciclo del cultivo. Se utilizaron plantas de tres variedades de papa Call White, Spunta y Santana cosechadas durante los años 2010, 2011 y 2012, respectivamente. Se evaluó el comportamiento de las temperaturas mínimas, medias y máximas así como la amplitud de estas, además se determinó el rendimiento promedio por planta en kilogramos y se infirió en t.ha -1 . Todo el procesamiento estadístico se realizó con el empleo del programa Statgraphycs v5.1 y los gráficos se realizaron con el programa Sigma Plot v3.1.

  9. Development of technique on the induction and selection of in vitro mutant lines (Potato, Solanum tuberosum L.)

    Energy Technology Data Exchange (ETDEWEB)

    Yoo, Jang Ryoel; Lee, Yeong Il; Song, Hee Seop; Kim, Jae Seong; Sin, In Cheol; Lee, Sang Jae; Lee, Ki Un; Lim, Yong Taek [Korea Atomic Energy Res. Inst., Taejon (Korea, Republic of)

    1993-09-01

    For the development of the technique on the plant tissue culture and application of nuclear technique in the in vitro mutation breeding, present research laid emphasis on the development of techniques of potato tissue culture, and on the induction and selection of radiation mutation. Another culture for haploid induction, optimum radiation dosage for cybrid formation of potato and mutation induction from in vitro cultured microtuber and plantlets were investigated for modelling the technique on the induction and selection of in vitro mutant lines. Inheritance stability of the selected mutants were also studied in field condition. In vitro system of micropropagation and selection of mutation was summarized.

  10. From introduced American weed to Cape Verde Islands endemic: the case of Solanum rigidum Lam. (Solanaceae, Solanum subgenus Leptostemonum)

    OpenAIRE

    Sandra Knapp; Maria Vorontsova

    2013-01-01

    Abstract A Solanum species long considered an American introduction to the Cape Verde Islands off the west coast of Africa is identified as Solanum rigidum , a member of the Eggplant clade of Old World spiny solanums ( Solanum subgenus Leptostemonum ) and is probably endemic to the Cape Verde Islands. Collections of this species from the Caribbean are likely to have been introduced from the Cape Verde Islands on slave ships. We discuss the complex nomenclatural history of this plant and provi...

  11. Palinologia de espécies de Solanum L. (Solanaceae A. Juss.) ocorrentes nas restingas do Estado do Rio de Janeiro, Brasil Palynology of species of Solanum L. (Solanaceae A. Juss.) from the restingas of Rio de Janeiro State, Brazil

    OpenAIRE

    Carla Patrícia Rodrigues Batista-Franklim; Vania Gonçalves-Esteves

    2008-01-01

    Foram estudados 21 táxons de Solanum L., com o objetivo de caracterizá-los palinologicamente e, assim, contribuir para a elaboração de um catálogo polínico da flora das restingas do Estado do Rio de Janeiro. Os táxons examinados foram Solanum affine Sendtn., Solanum americanum Mill. var. americanum,Solanum argenteum Dunal ex Poir., Solanum aturense Dunal, Solanum caavurana Vell., Solanum capsicoides All., Solanum carautae Carv., Solanum cordifolium Dunal, Solanum curvispinum Dunal, Solanum ec...

  12. Algunos componentes esteroidales del solanum havanense jacq

    Directory of Open Access Journals (Sweden)

    Mario J. Basterrechea Rey

    2000-01-01

    Full Text Available Un nuevo alcaloide esteroidal llamado acetil etiolina, ha sido aislado de las hojas, tallos y raices del Solanum havanense Jacq y su estructura corresponde con (25S-16a-acetoxi-22.26-epiminocolest-5.22(N-dien-3ß-ol.

  13. Solanum jamesii - new traits and hybrids

    Science.gov (United States)

    One of the two wild potato relatives native to the USA is Solanum jamesii (jam). The genebank has collected and studied over 120 samples since 1958. This species has been shown to have extreme late blight resistance, and its tubers have extremely long dormancy, high antioxidants, and compounds tha...

  14. Flowerlocation in Solanum dulcamara L. (Solanaceae

    Directory of Open Access Journals (Sweden)

    Irina Zhuravlyeva

    2013-01-01

    Full Text Available The morphology of inflorescence of Solanum dulcamara is studied. Pseudolateral location of inflorescence relatively to plant body is set, the absence of bracteae and the sympodial type of growing of branches are found out. From W. Troll point of view the inflorescence of nightshade is defined as the polytelica synflorescence – complex dichasium.

  15. Desempenho de cultivares nacionais de batata para produtividade de tubérculos

    Directory of Open Access Journals (Sweden)

    Giovani Olegario da Silva

    2014-10-01

    Full Text Available O objetivo deste trabalho foi verificar o desempenho dos cultivares de batata nacionais BRS Ana e BRS Clara, em comparação com os cultivares importados Ágata e Asterix, em relação à produtividade de tubérculos. Os experimentos foram realizados no município de Canoinhas, SC. Foram avaliados quatro cultivares de batata: BRS Ana, BRS Clara, Asterix e Ágata. O delineamento experimental foi em blocos casualizados, com quatro repetições. As parcelas continham quatro linhas com 20 plantas em cada linha. Cento e dez dias após o plantio foi realizada a colheita e, os tubérculos de cada parcela, avaliados para caracteres relacionados com a produtividade de tubérculos. Os dados foram submetidos à análise de variância e agrupamento de médias, por Skott & Knott, a 5% de probabilidade. Os cultivares de batata nacionais BRS Ana e BRS Clara foram mais produtivos do que os importados Asterix e Ágata; no entanto, são mais tardios, quanto ao ciclo vegetativo.

  16. A new species of spiny Solanum (Solanaceae) from Peru

    OpenAIRE

    Stephen Stern

    2014-01-01

    Abstract A new species of Solanum is described from Peru. Solanum junctum S. Stern & M. Nee, sp nov. is a member of the Torva clade of the spiny solanums (Leptostemonum clade). The narrow corolla lobes and recurved prickles of Solanum junctum are similar to species in the Micracantha clade, but Solanum junctum differs in its branched inflorescences and upright green fruits. These characteristics are shared with other members the Torva clade; within this section Solanum junctum is morphologica...

  17. Comparative analysis of Solanum stoloniferum responses to probing by the green peach aphid Myzus persicae and the potato aphid Macrosiphum euphorbiae

    Institute of Scientific and Technical Information of China (English)

    Adriana E.Alvarez; Viviana G.Broglia; Anahí M.Alberti D'Amato; Doret Wouters; Edwin van der Vossen; Elisa Garzo; W.Fred Tjallingii

    2013-01-01

    Plants protect themselves against aphid attacks by species-specific defense mechanisms.Previously,we have shown that Solanum stoloniferum Schlechtd has resistance factors to Myzus persicae Sulzer (Homoptera:Aphididae) at the epidermal/mesophyll level that are not effective against Macrosiphum euphorbiae Thomas (Homoptera:Aphididae).Here,we compare the nymphal mortality,the pre-reproductive development time,and the probing behavior of M.persicae and M.euphorbiae on S.stoloniferum and Solanum tuberosum L.Furthermore,we analyze the changes in gene expression in S.stoloniferum 96 hours post infestation by either aphid species.Although the M.euphorbiae probing behavior shows that aphids encounter more probing constrains on phloem activities-longer probing and salivation time-on S.stoloniferum than on S.tuberosum,the aphids succeeded in reaching a sustained ingestion of phloem sap on both plants.Probing by M.persicae on S.stoloniferum plants resulted in limited feeding only.Survival of M.euphorbiae and M.persicae was affected on young leaves,but not on senescent leaves of S.stoloniferum.Infestation by M.euphorbiae changed the expression of more genes than M.persicae did.At the systemic level both aphids elicited a weak response.Infestation of S.stoloniferum plants with a large number ofM.persicae induced morphological changes in the leaves,leading to the development of pustules that were caused by disrupted vascular parenchyma and surrounding tissue.In contrast,an infestation by M.euphorbiae had no morphological effects.Both plant species can be regarded as good host for M.euphorbiae,whereas only S.tuberosum is a good host for Mo persicae and S.stoloniferum is not.Infestation of S.stoloniferum by M.persicae or M.euphorbiae changed the expression of a set of plant genes specific for each of the aphids as well as a set of common genes.

  18. Lectotypification of Cavanilles' names in Solanum (Solanaceae

    Directory of Open Access Journals (Sweden)

    Knapp, Sandra

    2007-12-01

    Full Text Available Lectotypes are confirmed or designated here for the 13 names coined by Antonio José Cavanilles that were either described, or today are recognised as, members of the large genus Solanum (Solanaceae: Solanum betaceum, S. elaeagnifolium, S. fructotecto, S. lanceolatum, S. lentum, S. parviflorum, S. phyllanthum, S. pinnatum, S. pomiferum, S. pygmaeum, S. triquetrum, Triguera ambrosiaca and T. inodora. A brief introduction assesses the importance of Cavanilles to the botany of his time, and identifies difficulties in lectotypifying names coined by him. The currently accepted name for each taxon is given. Each typification is accompanied by a discussion of the reasoning behind the choice of specimen, and all lectotypes are illustrated.Se confirman o designan los lectótipos de 13 nombres de Antonio José Cavanilles que o bien fueron descritos dentro del género Solanum (Solanaceae o son actualmente reconocidos como parte del mismo: Solanum betaceum, S. elaeagnifolium, S. fructo- tecto, S. lanceolatum, S. lentum, S. parviflorum, S. phyllanthum, S. pinnatum, S. pomiferum, S. pygmaeum, S. triquetrum, Triguera ambrosiaca y T. inodora. Se incluye una breve introducción explicando la importancia de Cavanilles para la botánica de su tiempo, así como las dificultades que entraña lectotipificar las especies de este autor. Se indica el nombre aceptado para cada especie. Las tipificaciones se acompañan de una discusión, explicando la elección de los especímenes. Todos los lectótipos están ilustrados.

  19. A new alkaloid from Solanum cathayanum

    Institute of Scientific and Technical Information of China (English)

    Fan Cheng; Xiang Li; Jun Zhi Wang

    2008-01-01

    A new alkaloid compound I was obtained from Solanum cathayanum, a folk medicine of Hubei Province, together with a knownalkaloid, orotic acid ethyl ester. On the basis of spectroscopic data and chemical methods, I was identified as 8-hydroxy-3-methoxy-5H-pyrido[2,1-c]pyrazin-5-one. Compound 1 can inhibit the production of NO in peritoneal macrophage of mice induced with LPS.The content of NO was determined by enzyme methods.

  20. PHARMACOGNOSTIC STUDY OF KAKAMACHI (SOLANUM NIGRUM LINN

    Directory of Open Access Journals (Sweden)

    Jani Dilip K.

    2012-08-01

    Full Text Available Kakamachi (Solanum nigrum Linn belongs to family Solanaceae and is being used in Ayurveda in skin diseases and diabetes. It is being sold in the market under the common name Makoy. In order to ensure correct botanical standardization to remove the controversy, a detailed pharmacognostic study on whole plant of Solanum nigrum Linn has been carried out. The review on this drug shows that the drug is known since Vedic period. This is the plant amongst the few plants, which are used widely for purposes like Shaka Dravya (Vegetable drug. This study confirms that Solanum nigrum Linn fulfill the standard parameters, decided by the Ayurvedic Pharmacopoeial committee of India. The Physiochemical standards like ash value, alcohol soluble extracts, water soluble extracts etc, were also with the limits of the values mentioned in Pharmacopoeia. The heavy metals analysis of Kakamachi was carried out for Cadmium, Lead, Arsenic, etc., shows that the drug, is free from the abnormal levels of heavy metals. The qualitative study shows the presence of Saponins, Tannins and Alkaloids in Kakamachi. The standard monogram prepared concludes that these parameters could be useful for future standard.

  1. Produção da batata-doce adubada com esterco bovino e biofertilizante Yield of sweet potato (Ipomoea batatas (L.) Lam.) fertilized with cattle manure and biofertilizer

    OpenAIRE

    Ademar Pereira de Oliveira; Arthur Hennys Diniz Barbosa; Lourival Ferreira Cavalcante; Walter Esfraim Pereira; Arnaldo Nonato Pereira de Oliveira

    2007-01-01

    Objetivou-se avaliar a produção da batata-doce (Ipomoea batatas (L.) Lam.) adubada com esterco bovino e biofertilizante, conduziu-se um experimento no período de Janeiro a Junho de 2004, em NEOSSOLO REGOLÍTICO, psamítico típico, na Universidade Federal da Paraíba, em Areia-PB. O delineamento experimental empregado foi de blocos casualisados, com quatro repetições com os tratamentos distribuídos em arranjo fatorial 6 x 2, sendo seis doses de esterco bovino (0, 10, 20, 30, 40, 50 t ha-1) e ausê...

  2. Identification and characterisation of CYP75A31, a new flavonoid 3'5'-hydroxylase, isolated from Solanum lycopersicum

    Directory of Open Access Journals (Sweden)

    Bourgaud Frédéric

    2010-02-01

    Full Text Available Abstract Background Understanding the regulation of the flavonoid pathway is important for maximising the nutritional value of crop plants and possibly enhancing their resistance towards pathogens. The flavonoid 3'5'-hydroxylase (F3'5'H enzyme functions at an important branch point between flavonol and anthocyanin synthesis, as is evident from studies in petunia (Petunia hybrida, and potato (Solanum tuberosum. The present work involves the identification and characterisation of a F3'5'H gene from tomato (Solanum lycopersicum, and the examination of its putative role in flavonoid metabolism. Results The cloned and sequenced tomato F3'5'H gene was named CYP75A31. The gene was inserted into the pYeDP60 expression vector and the corresponding protein produced in yeast for functional characterisation. Several putative substrates for F3'5'H were tested in vitro using enzyme assays on microsome preparations. The results showed that two hydroxylation steps occurred. Expression of the CYP75A31 gene was also tested in vivo, in various parts of the vegetative tomato plant, along with other key genes of the flavonoid pathway using real-time PCR. A clear response to nitrogen depletion was shown for CYP75A31 and all other genes tested. The content of rutin and kaempferol-3-rutinoside was found to increase as a response to nitrogen depletion in most parts of the plant, however the growth conditions used in this study did not lead to accumulation of anthocyanins. Conclusions CYP75A31 (NCBI accession number GQ904194, encodes a flavonoid 3'5'-hydroxylase, which accepts flavones, flavanones, dihydroflavonols and flavonols as substrates. The expression of the CYP75A31 gene was found to increase in response to nitrogen deprivation, in accordance with other genes in the phenylpropanoid pathway, as expected for a gene involved in flavonoid metabolism.

  3. RNA-seq discovery, functional characterization, and comparison of sesquiterpene synthases from Solanum lycopersicum and Solanum habrochaites trichomes

    NARCIS (Netherlands)

    P.M. Bleeker; E.A. Spyropoulou; P.J. Diergaarde; P. Volpin; M.T.J. de Both; P. Zerbe; J. Bohlmann; V. Falara; Y. Matsuba; E. Pichersky; M.A. Haring; R.C. Schuurink

    2011-01-01

    Solanum lycopersicum and Solanum habrochaites (f. typicum) accession PI127826 emit a variety of sesquiterpenes. To identify terpene synthases involved in the production of these volatile sesquiterpenes, we used massive parallel pyrosequencing (RNA-seq) to obtain the transcriptome of the stem trichom

  4. Controle de plantas daninhas na cultura da batata Potato weed control by application of herbicides

    OpenAIRE

    Jeferson Zagonel; Marie Y. Reghin; Eloir Moresco

    1999-01-01

    O experimento foi conduzido na Fazenda Escola da UEPG, em Ponta Grossa-PR, em Latossolo Vermelho Escuro de textura média argilosa, com o objetivo de verificar a praticabilidade e a eficiência agronômica dos herbicidas metolachlor e metribuzin + metolachlor no controle de plantas daninhas na cultura da batata. O delineamento experimental foi de blocos ao acaso com nove tratamentos e quatro repetições, quais sejam: metolachlor (1,92; 2,88 e 3,84 kg i.a./ha); metribuzin + metolachlor (0,24 + 1,6...

  5. Microbial deterioration of white variety sweet potato (Ipomoea batatas) under different storage structures

    OpenAIRE

    Wisdom Amoa-Awua; Mary Obodai; Charles Tortoe

    2010-01-01

    Post-harvest protection of white variety sweet potato (Ipomoea batatas) has been dealt with rarely in the past, although it is comparatively easy to grow and has high consumer acceptability in Ghana. Microbial deterioration of sweet potato roots stored in three different storage structures was studied. The sweet potato roots initially cured for 7 and 14 days were stored in traditional, pit, and clamp storage structures for a maximum of 28 days. For the 7 days-cured sweet potato roots, the bac...

  6. The Optimal Extraction Parameters and Anti-Diabetic Activity of Flavonoids from Ipomoea Batatas Leaf

    OpenAIRE

    Li, Fenglin; Li, Qingwang; Gao, Dawei; Peng, Yong

    2009-01-01

    Extraction parameters of flavonoids from Ipomoea batatas leaf (FIBL) and anti-diabetic activity of FIBL on alloxan induced diabetic mice were studied. The optimal extraction parameters of FIBL were obtained by single factor test and orthogonal test, as follows: ethanol concentration 60 %, ratio of solvent to raw material 30, extraction temperature 75 ° and extraction time 1.5 h, while extraction yield of FIBL was 5.94 %. FIBL treatment (50, 100, and 150 mg/ kg body weight) for 28 days resulte...

  7. Resistência de genótipos de batata a Phthorimaea operculella (Zeller) Resistance of potato genotypes to by the potato tuber moth Phthorimaea operculella (Zeller)

    OpenAIRE

    Maria Teresa do Rêgo Lopes; José Djair Vendramim

    2001-01-01

    A traça Phthorimaea operculella (Zeller) é considerada atualmente uma das principais pragas da batata. O uso de resistência varietal para controle desse inseto é uma alternativa promissora, mas que ainda precisa ser melhor pesquisada. A resistência de genótipos de batata à traça foi avaliada, em condições de laboratório, analisando-se o efeito de folhas e tubérculos sobre a biologia do inseto. Comparou-se também o desenvolvimento da traça em folhas de batata e tomate. Os experimentos foram co...

  8. Osmotic dehydration of sweet potato (Ipomoea batatas in ternary solutions Desidratação osmótica de batata-doce (Ipomoea batatas em soluções ternárias

    Directory of Open Access Journals (Sweden)

    Graziella Colato Antonio

    2008-09-01

    Full Text Available The aim of this work was to evaluate the osmotic dehydration of sweet potato (Ipomoea batatas using hypertonic sucrose solutions, with or without NaCl, at three different concentrations, at 40 °C. Highest water losses were obtained when the mixture of sucrose and NaCl was used. The addition of NaCl to osmotic solutions increases the driving force of the process and it is verified that the osmotic dehydration process is mainly influenced by changes in NaCl concentration, but the positive effect of the salt-sucrose interaction on soluble solids also determined the decrease of solid gain when solutes were at maximum concentrations. Mass transfer kinetics were modeled according to Peleg, Fick and Page's equations, which presented good fittings of the experimental data. Peleg's equation and Page's model presented the best fitting and showed excellent predictive capacity for water loss and salt gain data. The effective diffusivity determined using Fick's Second Law applied to slice geometry was found to be in the range from 3.82 x 10-11 to 7.46 x 10-11 m²/s for water loss and from 1.18 x 10-10 to 3.38 x 10-11 m²/s for solid gain.O objetivo deste trabalho foi avaliar a desidratação osmótica da batata-doce (Ipomoea batatas utilizando soluções hipertônicas de sacarose e/ou NaCl, em três concentrações diferentes a 40 °C. As perdas de água mais elevadas foram obtidas quando se utilizou a mistura de sacarose e NaCl. A adição do NaCl às soluções osmóticas aumenta a força motriz do processo. Verifica-se que o processo de desidratação osmótica é principalmente influenciado pela mudança da concentração de NaCl, mas o efeito positivo da interação do sal com a sacarose também determinou a diminuição do ganho de sólidos quando os solutos estavam em concentrações máximas. As cinéticas de transferência de massa foram modeladas de acordo com as Equações de Peleg, de Fick e de Page, e apresentaram bons ajustes aos dados

  9. Identificación in silico de un grupo de secuencias ortólogas conservadas (COS de Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Christian Solís Calero

    2013-03-01

    Full Text Available En el presente trabajo se describe una serie de procedimientos bioinformáticos para la predicción de un grupo secuencias ortólogas conservadas (COS de Ipomoea batatas, así como la evaluación de su potencial utilidad para la generación de marcadores moleculares y estudios de diversidad en esta especie. Con ese propósito usando los programas BLAST X y TBLASTN se realizó una comparación reciproca por similaridad entre secuencias ESTs procedentes de librerías de cDNAs de Ipomoea batatas, propias o disponibles de modo público en la base de datos GenBank, con secuencias COS de Arabidopsis thaliana. La anotación funcional de las secuencias COS predichas en Ipomoea batatas se realizo usando los programas BLASTX, INTERPROSCAN y PSI-BLAST. Se obtuvieron en total 204 secuencias COS candidatos de Ipomoea batatas, siendo 16 secuencias provenientes de una librería generada a partir de raíces de reserva. Se evaluó de modo computacional el polimorfismo de las secuencias COS de raíces de reserva, obteniéndose SNPs en 8 secuencias, y secuencias repetidas en tandem en una de ellas.

  10. Utilizing wild relative ((Solanum viarum as resistant source to shoot and fruit borer in brinjal (Solanum melongena Linn.

    Directory of Open Access Journals (Sweden)

    L.Pugalendhi, D.Veeraragavathatham, S.Natarjan and S. Praneetha

    2010-07-01

    Full Text Available Brinjal (Solanum melongena Linn. has very important place in Indian curries and also in China, Japan and Southern Europianrecipies. High yield combined with good quality as well as resistance to shoot and fruit borer in a brinjal variety is the ultimateaim in the most of the brinjal breeding programme. Solanum viarum is closely related to Solanum melongena and both are crosscompatible. Therefore hybridization was undertaken in brinjal (Solanum melongena L. with Solanum viarum to transfer theresistance trait of shoot and fruit borer and combine the resistance trait with high yield of brinjal.. Hybridization was made andF1 hybrid plants were raised. Subsequently in each generation selfing was done followed by selection to obtain F9 generation. InF9 generation selection was done in the plants with high marketable yield along with very low or negligible shoot and fruit borerinfestation. The direct derivatives of EP 65 x Solanum viarum were evaluated upto F9 . Two recombinant progenies viz., 7 and 9were selected in F9 generation for carrying forward to the next generation based on their high marketable yield and the leastinfestation of shoot and fruit borer. Molecular study with RAPD primers also revealed the introgression of the genes from donorparent Solanum viarum to brinjal.

  11. Chemical constituents of Solanum buddleifolium Sendtn; Constituintes quimicos de Solanum buddleifolium Sendtn

    Energy Technology Data Exchange (ETDEWEB)

    Pinto, Francisco das Chagas L.; Torres, Maria da conceicao M.; Silveira, Edilberto R.; Pessoa, Otilia Deusdenia L., E-mail: opessoa@ufc.br [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil). Centro de Ciencias. Dept. de Quimica Organica e Inorganica; Braz-Filho, Raimundo [Universidade Estadual do Norte Fluminense, Campos, RJ (Brazil). Dept. de Quimica; Guedes, Maria Lenise da Silva [Universidade Federal da Bahia (UFBA), Ondina, BA (Brazil). Inst. de Biologia. Dept. de Botanica

    2013-10-01

    The chemical investigation of the stem EtOH extract of S. buddleifolium resulted in the isolation of terpenoids, amides, lignans and a steroidal alkaloid. Based on HRMS, IR and {sup 1}H and {sup 13}C NMR data analysis, the structures of the isolated compounds were identified as: 13-hydroxysolavetivone, betulinic acid, N-trans-caffeoyltyramine, N-trans-feruloyldopamine, N-trans-p-cumaroyltyramine, N-trans-feruloyltyramine, N-trans-feruloyl- 3'-O-methoxydopamine, alangilignoside C, isolariciresinol, polistachiol, (+)-(8R,7'S,8'S)-3{alpha}-O-({beta}-D-glucopiranosyl)-lioniresinol, (-)-(8S,7'R,8'R)-3{alpha}-O-({beta}-D-glucopiranosyl)-lioniresinol and solamargine. The occurrence of terpenoids and amides is common in Solanum, unlike lignans which are rare. The isolated lignans described in this work are reported for the first time in the genus Solanum. (author)

  12. Evaluation sensorielle du couscous de farine de manioc (Manihot esculenta, Crantz) substituée par celle de patate douce (Ipomoea batatas, Lam)

    OpenAIRE

    Amadou, NM.; Waingeh, NC.; Dung, MS.; Imele, H.

    2016-01-01

    Consumer Acceptance of Cassava (Manihot esculenta, Crantz) flour's fufu substituted by Sweet Potato (Ipomoea batatas, Lam) Flour. This study investigated the consumer acceptance of fufu made by substituting cassava (Manihot esculenta, Crantz) flour with sweet potato (Ipomoea batatas, Lam) flour at the Food Technology and Post-harvest laboratory of IRAD in Bambui, Cameroon. Four samples of Fufu flour samples F0, F1, F2 and F3 obtained by substituting cassava flour with 0%, 20%, 40% and 50% pot...

  13. PHYTOCHEMICAL EVALUATION OF THREE SPECIES OF SOLANUM L.

    Directory of Open Access Journals (Sweden)

    S. Gnana Sundari

    2013-06-01

    Full Text Available The identification of primary and secondary constituents has become the utmost important tool to know the active principles of various medicinal plants. The present study was aimed at identification and evaluation of certain phytochemicals of therapeutic importance in Solanum torvum, Solanum trilobatum and Solanum xanthocarpum of the genus Solanum L., used in folk and traditional medicine to cure respiratory disorders especially bronchial asthma. The five different aqueous and organic (chloroform, dimethyl sulfoxide, ethanol and methanol extracts of the leaves, flowers and fruits were used for identification of phytochemical constituents. The analysis revealed the presence of phytochemicals like alkaloids, flavonoids, saponins, steroids, tannins, terpenoids and phenolic compounds. Further, some of these phytochemicals were quantitatively estimated.

  14. STEROIDAL GLYCOSIDES FROM THE ROOTS OF SOLANUM MELONGENA L.

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    Stepan Shvets

    2009-12-01

    Full Text Available One new cholestane glycoside, six steroidal glycosides of spirostane series and one pregnane glycoside have been isolated from the roots of Solanum melongena L. for the first time. Their structures were determined by physico-chemical methods.

  15. Antidiarrheal activity of Solanum asterophorum in mice

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    Polyana Cristina Barros Silva

    2012-02-01

    Full Text Available Several species of Solanum are used in folk medicine to treat diarrhea. Therefore, the aim of this study was to investigate and compare possible antidiarrheal activity of methanol extracts from roots (Sast-MeOH R and leaves (Sast-MeOH L of Solanum asterophorum Mart., Solanaceae, in mice. Sast-MeOH R was shown to significantly and dose-relatedly inhibit the frequency of both solid (ED50 309.6±28.5 mg/kg and liquid (ED50 152.1±32.5 mg/kg stools. Conversely, Sast-MeOH L significantly inhibited solid stool frequency only when dosed at 500 and 750 mg/kg (48.7±7.4 and 42.3±9.8%, respectively, but also significantly and dose-relatedly inhibited liquid stools (ED50 268.4±35.2 mg/kg. Thus, Sast-MeOH R was twice as potent as Sast-MeOH L in diarrhea inhibition. Neither extracts (when dosed up to 500 mg/kg inhibited intestinal transit. However, both extracts significantly and dose-relatedly inhibited intestinal fluids, and Sast-MeOH R (ED50 38.3±10.4 mg/kg was again twice as potent as Sast-MeOH L (ED50 78.6±6.4 mg/kg. Results suggest that antidiarrheal effects of Sast-MeOH R and Sast-MeOH L involve changes on intestinal secretion. In addition, active metabolites with antidiarrheal activity may be more concentrated in the roots of this species. However further studies are needed to elucidate the action mechanism involved in this activity.

  16. RNA-seq discovery, functional characterization, and comparison of sesquiterpene synthases from Solanum lycopersicum and Solanum habrochaites trichomes

    OpenAIRE

    Bleeker, Petra M.; Spyropoulou, Eleni A; Diergaarde, Paul J.; Volpin, Hanne; De Both, Michiel T. J.; Zerbe, Philipp; Bohlmann, Joerg; Falara, Vasiliki; Matsuba, Yuki; Pichersky, Eran; Haring, Michel A.; Schuurink, Robert C.

    2011-01-01

    Solanum lycopersicum and Solanum habrochaites (f. typicum) accession PI127826 emit a variety of sesquiterpenes. To identify terpene synthases involved in the production of these volatile sesquiterpenes, we used massive parallel pyrosequencing (RNA-seq) to obtain the transcriptome of the stem trichomes from these plants. This approach resulted initially in the discovery of six sesquiterpene synthase cDNAs from S. lycopersicum and five from S. habrochaites. Searches of other databases and the S...

  17. Identification of enzyme activity quantitative trait loci in a Solanum lycopersicum x Solanum pennellii introgression line population

    OpenAIRE

    Steinhauser, Marie-Caroline; Steinhauser, Dirk; Gibon, Yves; Bolger, Marie; Arrivault, Stéphanie; Usadel, Björn; Zamir, Dani; Fernie, Alisdair R.; Stitt, Mark

    2011-01-01

    Activities of 28 enzymes from central carbon metabolism were measured in pericarp tissue of ripe tomato fruits from field trials with an introgression line (IL) population generated by introgressing segments of the genome of the wild relative Solanum pennellii (LA0716) into the modern tomato cultivar Solanum lycopersicum M82. Enzyme activities were determined using a robotized platform in optimized conditions, where the activities largely reflect the level of the corresponding proteins. Two e...

  18. Sweet Potato [Ipomoea batatas (L. Lam] Yield Influenced by Seedlings and Mulching

    Directory of Open Access Journals (Sweden)

    Bruno Novak

    2007-12-01

    Full Text Available Sweet potato [Ipomoea batatas (L. Lam] needs a yearly minimum of three month with air temperatures above 15º C for its growth and development. For the purpose of achieving the highest possible sweet potato yield during a relatively short vegetation period in continental part of Croatia, a trial with differently produced seedlings and polyethylene (PE mulch was set up. A trial was set up to study the production of seedlings from dormant sweet potato root sprouts by standard methods, and the most recent method of seedling production in containers, along with testing black PE-fi lm mulched soil, and uncovered soil. The method of seedlings production had significant effect on the yield. Higher mass of marketable roots was achieved by growing seedling with a substrate lump about the root, as compared to the seedlings produced by traditional procedure. Compared to the uncovered soil, a significantly higher yield of marketable roots was obtained with black PE-film mulch.

  19. Desenvolvimento de nanocompósitos à base de amido de batata

    OpenAIRE

    Luciana Macedo Brito; Maria Inês Bruno Tavares

    2013-01-01

    Nanocompósitos de amido de batata foram preparados pelo método de intercalação por solução, com a adição de argilas montmorilonita: organicamente modificada (Viscogel B8) e não modificada argila sódica (NT25) e de sílicas: modificada (R972) e não modificada (A200). Os nanocompósitos foram caracterizados pelas técnicas convencionais de difratometria de raios X e análise termogravimétrica. Foram caracterizados também por uma técnica denominada como não convencional, a ressonância magnética nucl...

  20. Some Proximate Properties Of Sweet Potato Ipomoea Batatas L As Influenced By Cooking Methods

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    Adepoju

    2015-03-01

    Full Text Available Abstract The effect of cooking methods on some proximate properties of sweet potato Ipomoea batatas L was investigated. With the initial properties of the fresh sweet potatoes sample A determined 600g each of samples B C and D were boiled peeled boiled unpeeled and roasted unpeeled respectively. The properties determined for the samples are moisture content ash fibre protein fat carbohydrate vitamins A and C. Data collected were analysed statistically to determine the effect of cooking methods on the properties of sweet potatoes. The results show that cooking methods used has no significant effects p0.05 on the moisture ash and crude fibre contents of cooked sweet potato. Cooking generally increases the protein content but decreases the vitamin C and amp946-carotene content. Cooking of sweet potatoes unpeeled has the highest protein and carbohydrate content it hence recommended for cooking sweet potatoes.

  1. Las plantas americanas en la obra de Charles de l'Écluse: primeras citas en las cartas de Juan de Castañeda

    OpenAIRE

    Ramón-Laca Menéndez de Luarca, Luis

    1999-01-01

    The Flemish botanist Charles de l'Écluse's works contain abundant references to American plants, for example the avocado (Persea americana Mill.), the sweet potato [Ipomoea batatas (L.) Lam.], the potato (Solanum tuberosum L.) and the tobáceo (Nicotiana tabacum L.). Although some of these plants were observed by L'Écluse himself during his expedition to the Iberian Península between 1564 and 1565, the majority of the American plants quoted in his works are related with shipments received from...

  2. Desempenho de clones elite de batata para caracteres agronômicos e de qualidade industrial

    Directory of Open Access Journals (Sweden)

    Giovani Olegario da Silva

    2015-02-01

    Full Text Available O objetivo deste trabalho foi verificar o desempenho de clones elite de batata, em relação a caracteres agronômicos e de qualidade industrial. Os experimentos foram realizados em Pelotas, RS, Canoinhas, SC, e Londrina, PR. Foi avaliado um conjunto de clones elite, pertencentes ao Programa de Melhoramento Genético de Batata, da Embrapa. O delineamento experimental foi o de blocos casualizados, com três repetições para Pelotas e Londrina e quatro para Canoinhas. Foram avaliados os caracteres massa total de tubérculos, massa comercial de tubérculos, número de tubérculos comerciais, percentagem de massa de tubérculos comerciais, massa média de tubérculos comerciais, peso específico, cor de fritura e ciclo vegetativo. A partir dos dados obtidos foram realizadas as análises de variância para cada local e teste de agrupamento de médias para cada caráter. Para identificação de clones apropriados ao mercado 'in natura', em que é fundamental o elevado potencial produtivo e precocidade, os clones mais promissores foram F80-03-06 e CL02-05, quando comparados à cultivar testemunha Ágata. Para o processamento industrial, em que o peso específico, a cor de fritura e o rendimento de tubérculo são caracteres importantes, destacou-se o clone F81-01-06, que apesar de não apresentar os maiores rendimentos totais, apresentou tubérculos grandes, ciclo vegetativo intermediário e boa aptidão para fritura, quando comparando-se ao cultivar testemunha Asterix.

  3. Sensorial, physical and chemical evaluation of bio-fortified Ipomoea batatas

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    Mara N.G. Santos

    2014-08-01

    Full Text Available Context: Bio-fortified sweet potato, Ipomoea batatas (L. Lamarck beta-carotene rich, has been included in the most vulnerable population’s diet with the purpose of reducing health disturbances associated with hipovitaminosis. Aims:To evaluate a physical and chemical analysis of total carotenes and beta-carotene and to determine the antioxidant potential as well as to carry out a sensorial analysis of the bio-fortified Ipomoea batatas (L. Lamarck (BDB in nature and processed. Methods: BDB was processed as sweet (in natura, paste and syrup and physicochemical characteristics were compared, total carotenoids, beta-carotene, antioxidant activity (DPPH and microbiological and sensory analyses were performed. Results:The analysis of total carotenoids, beta-carotene and anti-oxidant potential showed the following results: BDB in nature – 11.81 mg/100 g/440.5 mg/100 g/26.30%; sweet paste – 0.61 mg/100 g/53.5 mg/100 g/53.40% and savored syrup – 0.85 mg/100 g/46.0 mg/100 g/14.30%. The methods of conservation avoided the coliforms growth at 35 and 45°C, Staphylococcus coagulasa positivo, Bacillus cereus and Salmonella in all elaborated candies. The sensorial analysis didn’t detect significant differences among the syrup or paste. Conclusions: The conservation methods have an important function keeping the physical, chemical characteristics and sensorial of BDB, although they can influence in their chemical and nutritional composition, mainly in relation to the quantity of total carotenoids and beta-carotene.

  4. AMIDO DE BATATA DOCE (Ipomoea batatas L, Lam: II – CARACTERIZAÇÃO MORFOLÓGICA E ESTUDO DE ALGUMAS PROPRIEDADES FUNCIONAIS

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    JOSé PASCHOAL BATISTUTI

    2009-07-01

    Full Text Available

    RESUMO: O objetivo deste trabalho foi caracterizar morfologicamente e estudar a relação estrutura: função do grânulo de amido extraído de tubérculos, de diferentes cultivares de batata-doce (Ipomoea batatas L, Lam. Nas condições do experimento e mediante os resultados obtidos, observou-se que o amido dos diferentes cultivares estudados mostrou diferenças acentuadas na sua morfologia. Foram observadas, também, diferenças nas propriedades viscoamilográficas, devidas particularmente à heterogeneidade de forças envolvidas na manutenção da estrutura do grânulo. O amido obtido, dos cultivares estudados, mostrou maior resistência à desintegração mecânica, durante a gelatinização, quando comparado aos padrões comerciais testados. Apenas um cultivar mostrou pico de viscosidade máxima. PALAVRAS-CHAVE: Batata-doce; amido; funcionalidade; morfologia.

  5. On the origin of sweet potato (Ipomoea batatas (L.) Lam.) genetic diversity in New Guinea, a secondary centre of diversity

    OpenAIRE

    Roullier, C; Kambouo, R; Paofa, J; McKey, D; Lebot, V.

    2013-01-01

    New Guinea is considered the most important secondary centre of diversity for sweet potato (Ipomoea batatas). We analysed nuclear and chloroplast genetic diversity of 417 New Guinea sweet potato landraces, representing agro-morphological diversity collected throughout the island, and compared this diversity with that in tropical America. The molecular data reveal moderate diversity across all accessions analysed, lower than that found in tropical America. Nuclear data confirm p...

  6. Desempenho de genótipos de batata-doce submetidos ao efeito da calagem em Rio Largo-Alagoas

    Directory of Open Access Journals (Sweden)

    Islan Diego Espindula de Carvalho

    2015-09-01

    Full Text Available O objetivo desse trabalho foi avaliar o desempenho de genótipos de batata-doce submetidos ao efeito da calagem em Rio Largo-AL. O delineamento experimental utilizado foi o de blocos casualizados no esquema fatorial (7 x 2,  com sete genótipos de batata-doce e dois tipos de correção do solo, em três repetições. As variáveis avaliadas foram: Número de Raízes Comerciais (NRC; Número Total de Raízes (NTR; Comprimento de Raízes Comerciais (CRC; Diâmetro de Raízes Comerciais (DRC; Rendimento de Raízes Comerciais (RRC; Rendimento Total de Raízes (RTR e Peso Médio de Raízes Comerciais (PMRC. Dentre os genótipos de batata-doce avaliados no experimento, o Clone-06 apresentou o melhor desempenho, superando todos os genótipos avaliados, inclusive as testemunhas, Rainha de Penedo e Sergipana, tanto em produtividade, com rendimento médio de 15,79 t.ha-1de raízes comerciais, quanto em qualidade, apresentando dimensões de raízes comerciais dentro dos padrões de comercialização como Extra A. O desempenho dos genótipos, bem como a grande maioria das variáveis de batata-doce não foram influenciadas pela correção do solo.

  7. Characterization of Acylated Anthocyanins in Callus Induced From Storage Root of Purple-Fleshed Sweet Potato, Ipomoea batatas L

    OpenAIRE

    Terahara N.; Konczak I.; Ono H.; Yoshimoto M.; Yamakawa O.

    2004-01-01

    Four anthocyanins were isolated from a highly pigmented callus induced from the storage root of purple-fleshed sweet potato (Ipomoea batatas L) cultivar Ayamurasaki. The anthocyanins were respectively identified as cyanidin 3- O -(2- O -(6- O -( E )-caffeoyl- β -D-glucopyranosyl)- β -D-glucopyranoside)-5- O - β -D-glucopyranoside, cyanidin 3- O -(2- O -(6- O -( E )- p -coumaroyl- β -D-glucopyranosyl)-6- O -( E )-caffeoyl- β -D-glucopyranoside)-5- O -...

  8. Corte de tubérculos de batatinha (Solatium tuberosum L.: parte I - Estudos comparativos sôbre plantio de tubérculos inteiros, e cortados em "ápice" e "base"

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    Olavo J. Boock

    1947-01-01

    Full Text Available Several trials were conducted with potatoes (Solanum tuberosum L. to study the relative value of the apical and basal sets, as compared to whole seed tubers, since cutting potatoes before planting has been a matter of discussion. The experiments have been carried out on a high and on a low districts, say, respectively, at Joanópolis mountainous region, and at Taubaté flat lands, the latter on the right bank of Paraíba river, both in the State of São Paulo. Results have shown that on high lands, over 1,000 meters above sea level, the cutting process may be accomplished, though the basal sets have yielded less than apical ones, and whole tubers. Apical sets produced as well as whole tubers. At Taubaté district, aproximately 500 meters above sea level, where potato crop is usually grown from May to September in the dry season, on irrigated flat lands, after rice has been harvested, best results were secured from whole seed tubers. Apical and basal sets decayed easily, due probably to irrigation water ; for this reason they are not to be used.

  9. A new cryptically dioecious species of bush tomato (Solanum) from the Northern Territory, Australia

    OpenAIRE

    Christopher Martine; David E. Symon; Elizabeth C. Evans

    2013-01-01

    A new species of dioecious Solanum from the Australian “Dioicum Complex” of Solanum subgenus Leptostemonum is described. Solanum cowiei Martine sp. nov., is allied with other members of this problematic lineage, but differs in its slender leaves, limited armature and diminutive habit. The species was first segregated by botanists at the Northern Territory Herbarium as Solanum sp. Litchfield (I.D. Cowie 1428); and specimens representing this species have also been referred to by Sy...

  10. Listado anotado de Solanum L. (Solanaceae) en el Perú

    DEFF Research Database (Denmark)

    Särkinen, Tiina; Baden, Maria; Gonzáles, Paúl;

    2015-01-01

    The genus Solanum is among the most species-rich genera both of the Peruvian flora and of the tropical Andes in general. The present revised checklist treats 275 species of Solanum L., of which 252 are native, while 23 are introduced and/or cultivated. A total of 73 Solanum species (29% of native...

  11. Influence of heat treatment on the sensory and physical characteristics and carbohydrate fractions of french-fried potatoes (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Natalia Carvalho Montenegro de VASCONCELOS

    2015-09-01

    Full Text Available AbstractThe aim of this study was to analyze the impact that heat treatment with salts and freezing processes on the sensory, instrumental, and physico-chemical characteristics of fried potatoes of the Monalisa cultivar. The potatoes were blanched in distilled water (P; sodium chloride solution (B1; calcium chloride solution (B2, and a solution with both of these salts (B3. They were then pre-cooked and frozen for 24 hours and for 30 days. After frying, sensory characteristics were analyzed (color, texture, flavor, oiliness, along with overall preference and instrumental determinations of texture, color, and oil content. Further tests were conducted on the sample with the best results in the sensory analysis (B1, along with sample P as a control, to determine granule microstructure, carbohydrate fractions, glycemic index, and glycemic load. Blanching B3, despite reducing oil absorption and providing less oiliness, obtained lesser overall preference. Freezing for 30 days increased the lightness, except for when sodium chloride was used, which intensified the color yellow. The use of sodium chloride did not interfere with the type of starch granules, nor with the formation of resistant starch; however, longer freezing time reduced the glycemic index and concentrated the dietary fiber content. All samples exhibited low glycemic index and moderate glycemic loads.

  12. Climate Change and Potassium Effects Under Different N-Fertilization Input on Potato (Solanum tuberosum L.) Yield in a Long Term Field Experiment

    Science.gov (United States)

    László, Márton, ,, Dr.

    2010-05-01

    Climate change of Hungary was initiated about of 1850. Nowadays among the natural catastrophes, drought and flooding caused by over-abundant rainfall cause the greatest problems in field crop production. The droughts and the floods were experienced in the early eighties as well as today have drawn renewed attention to the analysis of this problem. The potato is demanding indicator crop of climate factors (temperature, rainfall) and soil nutrient status. Has a particularly high requirement for quantity of precipitation and for supply of soil potassium, nitrogen, phosphorus and magnesium. This paper reports the results achieved in the period from 1962 to 2001 of a long term small- plot fertilization experiment set up on acidic sandy brown forest soil at Nyírlugos in the Nyírség region in North- Eastern Hungary. Characteristics of the experiment soil were a pH (KCl) 4.5, humus 0.5%, CEC 5-10 mgeq 100g-1 in the ploughed layer. The topsoil was poor in all four macronutrient N, P, K and Mg. The mineral fertilization experiment involved 2 (genotype: Gülbaba and Aranyalma) x 2 (ploughed depth: 20 and 40 cm) x 16 (fertilizations: N, P, K, Mg) = 64 treatments in 8 replications, giving a total of 512 plots. The gross and net plot sizes were 10x5=50 m2 and 35.5 m2. The experimental designe was split-split-plot. The N levels were 0, 50, 100, 150 kg ha-1 year-1 and the P, K, Mg levels were 48, 150, 30 kg ha-1 year-1 P2O5, K2O, MgO in the form of 25% calcium ammonium nitrate, 18% superphosphate, 40% potassium chloride, and technological powdered magnesium sulphate. The forecrop every second year was rye. The groundwater level was at a depth of 2-3 m. From the 64 treatments, eight replications, altogether 512- experimental plots with 7 treatments and their 16 combinations are summarised of experiment period from 1962 to 1979. The main conclusions were as follows: 1. The experiment years (1962-1963, 1964-1965, 1966-1967, 1968-1969, 1970-1971, 1972-1973, 1974-1975, 1976-1977, 1978-1979) were characterised by frequent extremes of climate. Seven years had an average rainfall, one year had an over rainfall and one year had a very dry by Hungarian traditional and RISSAC-HAS new ecological standards. 2. The unfavorable effects of climate anomalies (drought, over-abundance of water in the topsoil) on the yield formation, yield quantity of potato depended decisively on the time of year when they were experienced and the period for which they lasted. 3. Droughts in the winter or summer half-year had much the same effect on yield. Precipitation deficiency in the winter could not be counterbalanced by average rainfall during the vegetation period, and its effect on the yield was similar to that of summer drought. 4. In vegetation periods poor in rainfall yield safety in potato can not be secured by 150 kg ha-1 potassium fertilization. It was also concluded that economic yields could not be achieved with poor N, P, K and Mg nutrient supply even with a normal quantity and distribution of rainfall. 5. Yield was influenced by rainfall to a greater extent than by 150 kg ha-1 potassium combinations (NK, NPK, NPKMg). 6. Drought and over rainfall negative effects were decreased by increasing N- doses and its combinations of potassium, phosphorous and magnesium from 13 to 32%. 7. With the help of regression analysis it was found the polynomial correlation between rainfall and yield could be observed in the case of NK (Y'=381.65-2.95x+0.0056x2, n=72, R2=0.95), NPK (Y'=390.87-3.07x+0.0060x2, n=72, R2=0.96) and NPKMg (Y'=390.45-3.06x+0.0059x2, n=72, R2=0.96) nutrition systems. The optimum yield ranges between 17-20 t ha-1 at 280-330 mm of rainfall. Key words: climate change, rainfall, potassium, potato, yield INTRODUCTION Climate change is now recognized as a serious environmental issue. The build up of greenhouse gases in the atmosphere and the inertia in trends in emissions means that we can expect significant changes for at least the next few decades and probably for the rest of this century. The urgent need is to understand what might be involved in ad

  13. BÚSQUEDA DE UN MEDIO DE CULTIVO PARA LA MICORRIZACIÓN In Vitro DE PLÁNTULAS DE PAPA (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Kalyanne Fernández Suárez

    2013-01-01

    Full Text Available El medio de cultivo constituye un eslabón clave para garantizar el establecimiento de la simbiosis micorrízica arbuscular en plantas in vitro, pues involucra a organismos con requerimientos nutricionales muy diferentes. Este trabajo se realizó con el objetivo de desarrollar un medio de cultivo a partir de modificaciones nutricionales realizadas al medio MS, que permitiera el establecimiento de la micorrización in vitro de plantas de papa con un estado nutricional adecuado. Se realizaron dos experimentos utilizando diferentes medios de cultivo (SRM, MS y 1⁄2 MS, así como variaciones en las concentraciones de nutrientes del medio MS empleando Diseños Completamente Aleatorizados. En estos medios se inocularon plántulas de papa, de entre siete y diez días de micropropagadas, con esporas de Glomus clarum, en un sistema autotrófico parcialmente in vitro. Se realizaron muestreos del desarrollo fúngico y de las plántulas a los 20, 30 y 40 días de cultivo. Se observó un comportamiento diferenciado tanto en los parámetros de crecimiento vegetal como fúngicos, dependiendo del medio de cultivo en el cual se desarrollaron, siendo en el medio E (1⁄4P-1⁄4N-1⁄4K con respecto a MS en el que se encontraron, de forma integral, los mayoresvalores de las variables evaluadas. Durante el tiempo de duración de los experimentos no se detectaron diferencias entre las plántulas inoculadas y las controles.

  14. Preliminary study on the effect of cobalt-60 gamma radiation on the conservation of potato tubers for consumption (Solanum tuberosum l.), during the storage period

    International Nuclear Information System (INIS)

    The object of the thesis is the study of the inhibiting effect of the sprouting of potato tubers, variety Loman, after Cobalt-60 gamma irradiation (with doses of 4, 6, 8, 10 and 12 Krads) as well as after treatment with the chemical inhibitor Isopropyl-N-Phenyl Carbonate (IPC). These treatments were applied to different groups of tubers, after 15 and 45 days from harvesting, stored under two illumination conditions: darkness (closed room), and indirect light (straw hut). The number, length and thickness of the sprouts, as well as the weight, humidity contents and infection of the tubers were recorded twice a month. A random 2x2x7x4 multifactorial design, with 3 repetitions, was used for the analysis and interpretation of the results. Variance and multiple regression analysis, as well as Tukey tests were performed on the IBM/370 computer of the San Carlos University (Guatemala). It was found that both the 4 and 6 Krad doses and the IPC treatments have no effect on the sprouting inhibition. The irradiation with the 8, 10, and 12 Krad doses inhibited the sprouting in an irreversible way, with insignificant loss of weight and moisture, regardless of the irradiation epoch and light condition. However, the tubers stored in darkness, showed ideal characteristics for consumption, and the 8 Krad dose, applied at 15 days after harvesting is concluded to be the best

  15. Evaluación y selección preliminar por rendimiento de tubérculo y potencial industrial de 36 clones de papa (solanum tuberosum l.)

    OpenAIRE

    Perilla C., Alexánder; Cifuentes O., Néstor; Rodríguez M., Luis E.; Ñustez L., Carlos E.

    2011-01-01

    Se evaluaron 36 clones de papa por calidad para la industria, rendimiento, respuesta a gota y precocidad, utilizando como testigos las variedades cultivadas para procesamiento industrial, Diacol Capiro, Diacol Monserrate e lCA Unica. Las variables evaluadas fueron porcentaje de tiras y hojuelas no quemadas, gravedad específica, rendimiento procesable, peso tamaño 2, área bajo la curva de la severidad de gota y días después de la siembra a maduración. El experimento se realizó en la Estación E...

  16. Electrochemical Determination of Low Molecular Mass Thiols Content in Potatoes (Solanum tuberosum Cultivated in the Presence of Various Sulphur Forms and Infected by Late Blight (Phytophora infestans

    Directory of Open Access Journals (Sweden)

    Rene Kizek

    2008-05-01

    Full Text Available In the present paper potato plants were cultivated in the presence of ammonium sulphate or elemental sulphur supplementation into the soil to reveal the effects of different sulphur forms on content of nitrogen, phosphorus, potassium, calcium, magnesium and sulphur, and yield of tubers. During the investigation of the influence of different sulphur forms on yield of potato tubers we did not observe significant changes. Average weight of tubers of control plants per one experimental pot was 355 g. Application of sulphur in both forms resulted in moderate potato tubers weight reduction per one experimental pot compared to control group; average value ranged from 320 to 350 g per one experimental pot. Further we treated the plants with two different supplementation of sulphur with cadmium(II ions (4 mg of cadmium(II acetate per kilogram of the soil. The significantly lowest cadmium content (p < 0.05 was determined in tissues of plants treated with the highest dosage of elemental sulphur (0.64 mg Cd/kg compared to control plants (0.82 mg Cd/kg. We also aimed our attention on the cadmium content in proteins, lipids or soluble carbohydrates and ash. Application of sulphate as well as elemental sulphur resulted in significant cadmium content reduction in lipid fraction compared to control plants. In addition to this we quantified content of low molecular mass thiols in potatoes tissues. To determine the thiols content we employed differential pulse voltammetry Brdicka reaction. After twelve days of the treatment enhancing of thiols level was observed in all experimental groups regardless to applied sulphur form and its concentration. Finally we evaluated the effect of sulphur supplementation on Phytophora infestans infection of potato plants.

  17. CARACTERIZACIÓN MORFOAGRONÓMICA DEL GERMOPLASMA CUBANO DE PAPA (Solanum tuberosum L.. EVALUACIÓN DE LAS ACCESIONES CULTIVADAS. PARTE II

    Directory of Open Access Journals (Sweden)

    J. G. Castillo

    2007-01-01

    Full Text Available En el Instituto Nacional de Ciencias Agrícolas, durante las campañas 2002-2003, 2003-2004 y 2004-2005, se estudiaron 288 accesiones cultivadas del germoplasma cubano de papa. Se realizó una caracterización morfoagronómica de las especies del banco, que incluyó los iguientes caracteres cualitativos: forma del tubérculo, color del tubérculo y profundidad de los ojos así como cuantitativos: rendimiento por planta (t.ha-1, masa promedio del tubérculo, número de tubérculos por planta y altura de la planta. Además, se evaluó el comportamiento ante el hongo A. solani y el virus del enrollamiento de la hoja (PLRV. Los caracteres cualitativos se expresan en porcentajes de presencia dentro de la colección. Para los cuantitativos se determinaron los estadísticos descriptivos, media, valores máximos y mínimos, desviación estándar y coeficiente de variación. Se realizó un análisis de componentes principales, para conocer la distribución de cada una de las especies dentro de la colección y su posible uso en el programa de mejoramiento cubano. Se encontró una alta variabilidad dentro de esta colección de variedades cultivadas para las condiciones cubanas; así como las fuentes de resistencia para el hongo A. solani y el PLRV. Se demostró, mediante esta caracterización, que existe un amplio pool de genes en esta colección, para ser utilizada más ampliamente en el programa de mejoramiento cubano.

  18. Postharvest application of organic and inorganic salts to control potato (Solanum tuberosum L.) storage soft rot: plant tissue-salt physicochemical interactions.

    Science.gov (United States)

    Yaganza, E S; Tweddell, R J; Arul, J

    2014-09-24

    Soft rot caused by Pectobacterium sp. is a devastating disease affecting stored potato tubers, and there is a lack of effective means of controlling this disease. In this study, 21 organic and inorganic salts were tested for their ability to control soft rot in potato tubers. In the preventive treatment, significant control of soft rot was observed with AlCl3 (≥66%) and Na2S2O3 (≥57%) and to a lesser extent with Al lactate and Na benzoate (≥34%) and K sorbate and Na propionate (≥27%). However, only a moderate control was achieved by curative treatment with AlCl3 and Na2S2O3 (42%) and sodium benzoate (≥33%). Overall, the in vitro inhibitory activity of salts was attenuated in the presence of plant tissue (in vivo) to different degrees. The inhibitory action of the salts in the preventive treatment, whether effective or otherwise, showed an inverse linear relationship with water ionization capacity (pK') of the salt ions, whereas in the curative treatment, only the effective salts showed this inverse linear relationship. Salt-plant tissue interactions appear to play a central role in the attenuated inhibitory activity of salts in potato tuber through reduction in the availability of the inhibitory ions for salt-bacteria interactions. This study demonstrates that AlCl3, Na2S2O3, and Na benzoate have potential in controlling potato tuber soft rot and provides a general basis for understanding of specific salt-tissue interactions. PMID:25174721

  19. Identification and reproducibility of diagnostic DNA markers for tuber starch and yield optimization in a novel association mapping population of potato (Solanum tuberosum L.)

    OpenAIRE

    Schönhals, E. M.; Ortega, F.; Barandalla, L.; Aragones, A.; Ruiz de Galarreta, J.I.; Liao, J.-C.; Sanetomo, R.; Walkemeier, B.; Tacke, E.; Ritter, E.; Gebhardt, C.

    2016-01-01

    Key message SNPs in candidate genes Pain - 1, InvCD141 (invertases), SSIV (starch synthase), StCDF1 (transcription factor), LapN (leucine aminopeptidase), and cytoplasm type are associated with potato tuber yield, starch content and/or starch yield. Abstract Tuber yield (TY), starch content (TSC), and starch yield (TSY) are complex characters of high importance for the potato crop in general and for industrial starch production in particular. DNA markers associated with superior alleles of ge...

  20. CARACTERIZACIÓN Y CALIDAD DE UN ABONO ORGÁNICO FERMENTADO aof PREPARADO CON RESIDUOS DEL PROCESO DE INDUSTRIALIZACIÓN DE LA PAPA (Solanum tuberosum L

    Directory of Open Access Journals (Sweden)

    Francisco García

    2009-12-01

    Full Text Available La producción de abonos orgánicos en Colombia se ha incrementado en los últimos años buscando dar un manejo adecuado a los desechos biodegradables generados por los procesos agroindustriales; el presente trabajo evaluó la calidad de composición mineral, caracterización del producto y población microbiana de un AOF fabricado a partir de los desechos generados en la transformación industrial de la papa. En el ensayo se hicieron 2 tratamientos: T1 consistió en la mezcla de 90% de material orgánico (residuo de papa 80%, aserrín 5%, desperdicios de molinería 3% y melaza 2% más la adición de minerales en forma de carbonatos, fosfatos y sulfatos en razón del 10%, en T2 solo se mezclo el material orgánico. El ensayo demostró que este material con 75% de humedad genera una fermentación anaeróbica con olor ácido muy fuerte, que decrece en la medida en que se pierde humedad, quedando la mezcla en un volumen de 50% y cambiando el olor al de una fermentación alcohólica. Respecto a las características del producto final el T2 mostró valores más altos de pH, COO, CIC, CRH y C/N, demostrando que el residuo de papa tiene un buen comportamiento como abono orgánico. De otra parte la adición de minerales eleva las cantidades de estos, que están contenidas de forma natural en el sustrato, dando al compuesto el valor agregado de un porcentaje más alto de elementos disponibles; en ambos casos la oferta de nutrientes y microorganismos es favorable para la nutrición vegetal.

  1. CARACTERIZACIÓN Y CALIDAD DE UN ABONO ORGÁNICO FERMENTADO aof PREPARADO CON RESIDUOS DEL PROCESO DE INDUSTRIALIZACIÓN DE LA PAPA (Solanum tuberosum L)

    OpenAIRE

    Francisco García; Pilar Gil; Alberto Carrillo

    2009-01-01

    La producción de abonos orgánicos en Colombia se ha incrementado en los últimos años buscando dar un manejo adecuado a los desechos biodegradables generados por los procesos agroindustriales; el presente trabajo evaluó la calidad de composición mineral, caracterización del producto y población microbiana de un AOF fabricado a partir de los desechos generados en la transformación industrial de la papa. En el ensayo se hicieron 2 tratamientos: T1 consistió en la mezcla de 90% de material orgáni...

  2. Aplicaciones de la guía agroclimática de Matanzas en el cultivo de la papa (Solanum tuberosum, L

    Directory of Open Access Journals (Sweden)

    Niliám Fernández Rosado

    2010-11-01

    Full Text Available Los informes del Panel Intergubernamental de Cambio Climático indican que el cambio climático será una realidad para el futuro y, las anomalías en el comportamiento del clima, detectadas por el Centro Nacional del Clima, sugieren que los Centros Meteorológicos Provinciales deben estar preparados para dar respuesta a las demandas de los diversos organismos para la adopción de medidas de adaptación a estos inminentes cambios. Como parte de esta preparación, existe la necesidad de la actualización de la información climática presente en la Guía Agroclimática de Matanzas, elaborada con la información de las estaciones meteorológicas del período 1967 a 1991, la que surgió principalmente del hecho que Matanzas, provincia eminentemente agrícola, con diversos centros de investigación científica, universidades y otros, necesitaban de la información meteorológica especializada, con el propósito de resolver diversos problemas de la producción, la investigación, la docencia y los servicios; dentro de ellos tenemos el establecimiento de las épocas óptimas de siembra de los cultivos, introducción de nuevas variedades, caracterizaciones agroclimáticas y otros. Desde entonces, nuevas áreas fueron asignadas para la agricultura, es una realidad la municipalización de las universidades y otras entidades científico-técnicas, que requieren de la información climática reciente. La presente actualización abarca desde 1992 hasta 2007. Este resultado activará aún más la oferta de los servicios del Centro Meteorológico Provincial a las entidades interesadas, la elevación del intercambio de información entre los organismos interesados y la participación en la búsqueda de soluciones a los problemas climáticos actuales, haciendo sostenible la producción agrícola.

  3. Influence of heat treatment on the sensory and physical characteristics and carbohydrate fractions of french-fried potatoes (Solanum tuberosum L.)

    OpenAIRE

    Natalia Carvalho Montenegro de VASCONCELOS; Silvana Magalhães SALGADO; Alda Verônica Souza LIVERA; Samara Alvachian Cardoso de ANDRADE; Michelle Galindo de OLIVEIRA; Tânia Lucia Montenegro STAMFORD

    2015-01-01

    AbstractThe aim of this study was to analyze the impact that heat treatment with salts and freezing processes on the sensory, instrumental, and physico-chemical characteristics of fried potatoes of the Monalisa cultivar. The potatoes were blanched in distilled water (P); sodium chloride solution (B1); calcium chloride solution (B2), and a solution with both of these salts (B3). They were then pre-cooked and frozen for 24 hours and for 30 days. After frying, sensory characteristics were analyz...

  4. Partitioning of photoassimilates by potato plants (Solanum tuberosum L.) as influenced by irradiance. II. Partitioning patterns by four clones grown under high and low irradiance

    International Nuclear Information System (INIS)

    This paper is the second in a three-part series describing the influence of varied irradiance on growth and photoassimilate partitioning by potato plants. Four clones (Russet Burbank, Lemhi Russet, A66107-51, and A6948-4) were grown under two light regimes: a) high light levels (HL) of 500 to 1200 μE m-2 s-1, varying with changes in natural sunlight and time of day, and b) low light levels (LL) at approximately one quarter of high light (21 to 28%). Three weeks after tuber initiation, the most recently-matured leaf was labelled with 14CO2, and plants were harvested: 1) one day after labelling, and 2) two weeks after labelling. Plants of all clones responded to the low light levels in a similar way by: 1) changing some morphological characteristics, 2) decreasing biomass accumulation and tuber yield, and 3) changing the sink-source relationship by promoting growth of leaves and stems at the expense of tubers. However, there were evident clonal differences in reactions to growth under low light; e.g., Lemhi Russet appeared to be most sensitive to light stress, while clones A66107-51 and A6848-4 were much less sensitive. No matter what the prior light history (HL or LL), clone A6948-4 was able to maintain higher rates of photosynthesis than the other clones at all light levels between 200 and 1200 μE m-2 s-1. This study showed that the potential exists to breed for cuttivars that can maintain higher rates of photosynthesis and higher tuber yield under low light levels. (author)

  5. ESTIMACIÓN DE LA SUPERFICIE FOLIAR EN DOS VARIEDADES DE PAPA (Solanum tuberosum L. POR MÉTODOS NO DESTRUCTIVOS

    Directory of Open Access Journals (Sweden)

    Eduardo Jerez Mompie

    2014-01-01

    Full Text Available El trabajo se desarrolló en el Instituto Nacional de Ciencias Agrícolas con el objetivo de determinar una función matemática a partir de las medidas lineales de las hojas de papa para estimar la superficie foliar de la planta de manera no destructiva. Se utilizaron plantas de dos variedades de papa Call White y Santana colectadas a los 40, 60 y 70 días después de la plantación, para asegurar que existieran hojas de distintos tamaños. Una vez realizadas las medidas lineales de las hojas (largo y ancho se procedió a determinar la superficie foliar real de la hoja con el empleo de un integrador de superficie foliar AM300 y a partir de estas variables se establecieron las regresiones lineales correspondientes al área real de cada hoja con cada una de las longitudes obtenidas y el producto de ellas. Se tuvo en cuenta el resultado del coeficiente de correlación y de determinación para escoger la función lineal que con mayor exactitud estima la superficie foliar de la hoja. La ecuación obtenida a partir del producto del largo por el ancho resultó la de mayor coeficiente de determinación en ambas variedades.

  6. A simple method to estimate vegetation indices and crop canopy factors using field spectroscopy for solanum tuberosum during the whole phenological cycle

    Science.gov (United States)

    Perdikou, S.; Papadavid, G.; Hadjimitsis, M.; Hadjimitsis, D.; Neofytou, N.

    2013-08-01

    Field spectroscopy is a part of the remote sensing techniques and very important for studies in agriculture. A GER-1500 field spectro-radiometer was used in this study in order to retrieve the necessary spectrum data of the spring potatoes for estimating spectral vegetation indices (SVI's). A field campaign was undertaken from September to the end of November 2012 for the collection of spectro-radiometric measurements. The study area was in the Mandria Village in Paphos district in Cyprus. This paper demonstrates how crop canopy factors can be statistically related to remotely sensed data, namely vegetation indices. The paper is a part of an EU cofounded project regarding estimating crop water requirements using remote sensing techniques and informing the farmers through 3G smart telephony.

  7. Comparing effects of low levels of herbicides on greehouse- and field-grown potatoes (Solanum tuberosum L.), soybeans (Glycine max L.) and peas (Pisum sataivum L.)

    Science.gov (United States)

    While laboratory toxicology tests are generally easy to perform, cost effective and readily interpreted, they have been questioned for their environmental relevance. In contrast, field tests are considered realistic while producing results that are difficult to interpret and exp...

  8. EVALUACIÓN DE UNA MUESTRA REPRESENTATIVA DEL GERMOPLASMA CUBANO DE PAPA (Solanum tuberosum L. PARA CALIDAD DEL TUBÉRCULO

    Directory of Open Access Journals (Sweden)

    Juan G. Castillo

    2013-01-01

    Full Text Available El desarrollo de variedades de papa con una mayor calidad del tubérculo es hoy uno de los retos fundamentales del programa de mejoramiento cubano. Para ello es imprescindible una evaluación detallada de los principales caracteres que afectan, en alguna medida, este importante atributo. Por tal motivo, el objetivo del trabajo estuvo enfocado hacia la evaluación de un grupo de variedades de papa, representativas del germoplasma cubano, mediante los indicadores de calidad del tubérculo para ser utilizadas como progenitores en un programa de mejoramiento genético. Se estudiaron 70 variedades cultivadas del banco de germoplasma, evaluando los caracteres relacionados con la calidad del tubérculo como: gravedad específica, masa seca, azúcares reductores, almidón, masa promedio, diámetro longitudinal y ecuatorial y evaluación sensorial. Fueron determinados los estadígrafos descriptivos media, desviación estándar, valores máximos y mínimos y coeficiente de variación para todos los caracteres cuantitativos estudiados. Se realizó un análisis de componentes principales, mediante una representación biplot, para conocer la agrupación de las accesiones y seleccionar las de mejor comportamiento para la calidad del tubérculo. Los resultados mostraron la existencia, en la colección, de variedades con buen comportamiento para la calidad del tubérculo (22,9 % del total, con valores de masa seca superior al 19 %, contenido de almidón por encima del 12 % y bajo contenido de azúcares reductores (< 0,5 %. Se propone utilizar a las mismas como progenitores en un programa de mejoramiento para procesamiento industrial o directamente en la producción. Dentro de ellas se destacaron las variedades Atlantic, Bellini, Cal White, Derby, Maranca, Romano, Santana y Slaney y los clones cubanos 1-10-96, 2-31-95, 4-6-93 y 9-32-93.

  9. Curdlan β-1,3-Glucooligosaccharides Induce the Defense Responses against Phytophthora infestans Infection of Potato (Solanum tuberosum L. cv. McCain G1) Leaf Cells

    OpenAIRE

    Li, Jing; Zhu, Li; Lu, Guangxing; Zhan, Xiao-Bei; Lin, Chi-Chung; Zheng, Zhi-Yong

    2014-01-01

    Activation of the innate immune system before the invasion of pathogens is a promising way to improve the resistance of plant against infection while reducing the use of agricultural chemicals. Although several elicitors were used to induce the resistance of potato plant to microbial pathogen infection, the role of curdlan oligosaccharide (CurdO) has not been established. In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicita...

  10. Identification and characterization of miRNAome in root, stem, leaf and tuber developmental stages of potato (Solanum tuberosum L.) by high-throughput sequencing

    OpenAIRE

    Lakhotia, Nisha; Joshi, Gopal; Bhardwaj, Ankur R.; Katiyar-Agarwal, Surekha; Agarwal, Manu; Jagannath, Arun; Goel, Shailendra; Kumar, Amar

    2014-01-01

    Background MicroRNAs (miRNAs) are ubiquitous components of endogenous plant transcriptome. miRNAs are small, single-stranded and ~21 nt long RNAs which regulate gene expression at the post-transcriptional level and are known to play essential roles in various aspects of plant development and growth. Previously, a number of miRNAs have been identified in potato through in silico analysis and deep sequencing approach. However, identification of miRNAs through deep sequencing approach was limite...

  11. Respuesta de diferentes poblaciones de spongospora subterránea f. sp. subterranea a la rotación entre dos variedades de papa (solanum tuberosum ssp. andigena).

    OpenAIRE

    Jaramillo Villegas, Sonia; Botero Herrera, Juan Manuel

    2011-01-01

    En una zona papera del Oriente de Antioquia - Colombia, se realizaron tres experimentos, para conocer el comportamiento de las variedades ICA Puracé, considerada por los agricultores, como resistente a la sarna polvosa de la papa, y la variedad Diacol Capiro susceptible. Se pretendió evaluar si hay un efecto de la rotación, entre las dos variedades con diferentes poblaciones de Spongospora subterranea f sp. subterranea sobre la sarna polvosa. Se utilizaron quistosoros de 23 poblaciones de S. ...

  12. Effects of Satureja hortensis oil treatments and exogenous H2O2 on Potato virus Y (PVY) infected Solanum tuberosum L. plants under drought conditions

    OpenAIRE

    Carmen Liliana BADARAU; Nicoleta CHIRU; Florentina DAMSA; Andreea NISTOR

    2012-01-01

    Effects of treatments with Satureja hortensis essential oils and H2O2 were evaluated in plants testing positive after Potato virus Y (PVY) infection, under drought conditions. In vitro PVY infected and uninfected plants were transfered to a green-house, injected with a Satureja hortensis essential oils suspension and sprayed twice a week with H2O2 (1 mM, pH 5.6). The treatments enhanced minitubers weight and starch content in PVY infected plants. Under drought conditions, minitubers produced...

  13. Effects of Satureja hortensis oil treatments and exogenous H2O2 on Potato virus Y (PVY infected Solanum tuberosum L. plants under drought conditions

    Directory of Open Access Journals (Sweden)

    Carmen Liliana BADARAU

    2012-11-01

    Full Text Available Effects of treatments with Satureja hortensis essential oils and H2O2 were evaluated in plants testing positive after Potato virus Y (PVY infection, under drought conditions. In vitro PVY infected and uninfected plants were transfered to a green-house, injected with a Satureja hortensis essential oils suspension and sprayed twice a week with H2O2 (1 mM, pH 5.6. The treatments enhanced minitubers weight and starch content in PVY infected plants. Under drought conditions, minitubers produced by infected and treated plants had significantly more starch than the controls. The treatments had positive effects on infected minitubers, such as weight, reduction of number, starch content, sprouting and tolerance to drought. A signal role for Satureja hortensis essential oils and hydrogen peroxide (H2O2 in lessenning symptoms is suggested.

  14. Efecto de antioxidantes y señalizadores en plantas de papa (Solanum tuberosum L. infectadas con Candidatus Liberibacter solanacearum bajo condiciones de invernadero

    Directory of Open Access Journals (Sweden)

    Isidro Humberto Almeyda León

    2014-12-01

    Full Text Available In Mexico, the losses caused by the purple top syndrome in potato range from 30 to 95%. This syndrome has been greatly associated with the bacterium Candidatus Liberibacter solaneacearum, which produces reduction in yield and in crop quality, the tubers have internal browning, which is not desirable either for fresh consumption or for the industry. Present study was conducted to evaluate the efficiency of three products that act as antioxidants and / or signs to reduce damage on potato caused by Ca. L. solanacearum under greenhouse conditions. Plants uninfected and infected with the bacterium, produced in vitro were used. The products evaluated were: dehydroascorbic acid (600 ppm, ascorbic acid (600 ppm and hydrogen peroxide (1 mM, and were applied to infected and uninfected plants twice a week. Infected and uninfected plants without application of antioxidants were used as control treatments. To avoid experimental error in the application of the products evaluated the experimental design was a randomized complete block. A reduction of potato damage by Ca. L solaneacearum was registered, and there were significant differences among treatments in the tubers production. The largest tuber number in treatments that included uninfected plants was obtained in the hydrogen peroxide application, which exceeded a 33 %, 48 % and 59 % to plants treated with dehydroascorbic acid, with ascorbic acid and the controls respectively. Similarly, the largest tuber number in treatments included infected plants was obtained by hydrogen peroxide, and it exceeded a 33 %, 17 % and 67 % to treatments with dehydroascorbic acid, with ascorbic acid and the controls respectively. These results show a potential effect of the products evaluated to protect potato plants against Ca. L. solanacearum, although its function is not to diminish the growth or development of bacteria.

  15. Chloroform fraction of Solanum tuberosum L. cv Jayoung epidermis suppresses LPS-induced inflammatory responses in macrophages and DSS-induced colitis in mice.

    Science.gov (United States)

    Lee, Seung-Jun; Shin, Ji-Sun; Choi, Hye-Eun; Lee, Kyoung-Goo; Cho, Young-Wuk; An, Hyo-Jin; Jang, Dae Sik; Jeong, Jin-Cheol; Kwon, Oh-Keun; Nam, Jung-Hwan; Lee, Kyung-Tae

    2014-01-01

    In this study, the authors investigated the molecular mechanism underlying the antiinflammatory effects of the chloroform fraction of the peel of 'Jayoung' (CFPJ), a color-fleshed potato, on lipopolysaccharide (LPS)-induced RAW 264.7 macrophages and in mice with dextran sulfate sodium (DSS)-induced colitis. CFPJ inhibited the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the transcription level, and attenuated the transcriptional activity of nuclear factor-κB (NF-κB) by reducing the translocation of NF-κB depending on degradation of inhibitory κB-α (IκB-α). Furthermore, CFPJ attenuated the phosphorylations of mitogen-activated protein kinase kinases3/6 (MKK3/6) and of p38. In colitis model, CFPJ significantly reduced the severity of colitis and the productions and protein levels of pro-inflammatory mediators in colonic tissue. These results suggest that the anti-inflammatory effects of CFPJ are associated with the suppression of NF-κB and p38 activation in macrophages, and support its possible therapeutic role for the treatment of colitis. PMID:24184733

  16. Modulation of the cellulose content of tuber cell walls by antisense expression of different potato (Solanum tuberosum L.) CesA clones

    NARCIS (Netherlands)

    Oomen, R.J.F.J.; Tzitzikas, E.; Bakx, E.J.; Straatman-Engelen, I.; Bush, M.S.; Mccann, M.C.; Schols, H.A.; Visser, R.G.F.; Vincken, J.P.

    2004-01-01

    Four potato cellulose synthase (CesA) homologs (StCesA1, 2, 3 and 4) were isolated by screening a cDNA library made from developing tubers. Based on sequence comparisons and the fact that all four potato cDNAs were isolated from this single cDNA-library, all four StCesA clones are likely to play a r

  17. Modulation of the cellulose content of tuber cell walls by antisense expression of different potato (Solanum tuberosum L.) CesA clones.

    Science.gov (United States)

    Oomen, Ronald J F J; Tzitzikas, Emmanouil N; Bakx, Edwin J; Straatman-Engelen, Irma; Bush, Maxwell S; McCann, Maureen C; Schols, Henk A; Visser, Richard G F; Vincken, Jean-Paul

    2004-03-01

    Four potato cellulose synthase (CesA) homologs (StCesA1, 2, 3 and 4) were isolated by screening a cDNA library made from developing tubers. Based on sequence comparisons and the fact that all four potato cDNAs were isolated from this single cDNA-library, all four StCesA clones are likely to play a role in primary cell wall biosynthesis. Several constructs were generated to modulate cellulose levels in potato plants in which the granule-bound starch synthase promoter was used to target the modification to the tubers. The StCesA3 was used for up- and down-regulation of the cellulose levels by sense (SE-StCesA3) and antisense (AS-StCesA3) expression of the complete cDNA. Additionally, the class-specific regions (CSR) of all four potato cellulose synthase genes were used for specific down-regulation (antisense) of the corresponding CesA genes (csr1, 2, 3 and 4). None of the transformants showed an overt developmental phenotype. Sections of tubers were screened for altered cell wall structure by Fourier Transform Infrared microspectroscopy (FTIR) and exploratory Principal Component Analysis (PCA), and those plants discriminating from WT plants were analysed for cellulose content and monosaccharide composition. Several transgenic lines were obtained with mainly decreased levels of cellulose. These results show that the cellulose content in potato tubers can be reduced down to 40% of the WT level without affecting normal plant development, and that constructs based on the CSR alone are specific and sufficient to down-regulate cellulose biosynthesis. PMID:15003416

  18. Efeito do nitrogênio, fósforo e potássio na adubação da batatinha - Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    O. J. Boock

    1950-08-01

    Full Text Available Five potatoes fertilizers trials were carried out. in diffeient soil types of the State of São Paulo with the purpose to study the effect of nitrogen, phosphoric acid and potash, on tuber production. It was found that nitrogen increased plant growth, intensified green color of foliage and retarded maturity. Nitrogen also increased susceptibility to fungus diseases. Phosphoric acid increased yield and shortened the vegetative period. Potassium induced a light -green color of the foliage, but otherwise had almost no influence on plant development. Phosphoric acid was the most important factor in increasing tuber production : nitrogen was second and potassium, third. A response to potassium was noticed only when this element was applied together with phosphorus and nitrogen.

  19. The evolution of the Solanum tuberosum L. neoplantles cultivated in vitro on cuture media supplimented with bioactive magneto-fluidic nanocomposites

    OpenAIRE

    Anca BACIU; Adriana PETRUS-VANCEA; Rodica ZEHAN

    2007-01-01

    During the last decades the bioactive magnetofluid nanocomposites proved both their unique performances and their high potential. Everywhere nanotechnologies gain ground being economic systems having a controlled period of the induced effect, of the planed biodegradation and of the used quantities. Nanotechnologies are applied in biology. They are of beneficed effects in plant and animal protection. The bioactive magnetofluid nanocomposites used for plants are applied in the vegetation phase ...

  20. Studies regarding the effects of Rosmarinus officinalis oil treatments in healthy and potato virus Y (PVY) infected plants Solanum tuberosum L.

    OpenAIRE

    Carmen Liliana BĂDĂRĂU; Angela MĂRCULESCU; Nicoleta CHIRU; Florentina DAMŞA; Nistor, Andreea

    2010-01-01

    The potato virus Y cause loss in yield and quality of tubers. Hydrogen peroxide, ascorbic acid and antioxidants such as rosmarinic acid present in oils extracted from Rosmarinus officinalis plants are implicated in signaling against stress. The effects of these chemicals on tuber yield and pigments content were evaluated in plants testing positive after virus mechanical infection. Without chemical treatment, positive plants showed significant reductions in leaf pigments content and tuber weig...

  1. Penetapan Kadar Timbal dan Kadmium pada Kentang (Solanum Tuberosum L) yang Tumbuh di Lahan Gunung Berapi Sinabung dengan Metode Spektrofotometri Serapan

    OpenAIRE

    Zulfikar

    2014-01-01

    Potato is a source of carbohydrate which also contains quite high vitamin and mineral. In Indonesia, potato is usually sold fresh and processed for example in potato chips. The purpose of this research is to know the lead and cadmium content in potato which grows on Sinabung volcanic area. Methods used in this assay were qualitative identification using color reaction by dithizone 0.005% and content assay using graphite furnace atomic absorption spectrophotometry method at wavelength 283.3...

  2. Vacuolar Invertase Gene Silencing in Potato (Solanum tuberosum L.) Improves Processing Quality by Decreasing the Frequency of Sugar-End Defects

    OpenAIRE

    Xiaobiao Zhu; Craig Richael; Patrick Chamberlain; Busse, James S; Alvin J. Bussan; Jiming Jiang; Paul C Bethke

    2014-01-01

    Sugar-end defect is a tuber quality disorder and persistent problem for the French fry processing industry that causes unacceptable darkening of one end of French fries. This defect appears when environmental stress during tuber growth increases post-harvest vacuolar acid invertase activity at one end of the tuber. Reducing sugars produced by invertase form dark-colored Maillard reaction products during frying. Acrylamide is another Maillard reaction product formed from reducing sugars and ac...

  3. Vacuolar invertase gene silencing in potato (Solanum tuberosum L.) improves processing quality by decreasing the frequency of sugar-end defects.

    Science.gov (United States)

    Zhu, Xiaobiao; Richael, Craig; Chamberlain, Patrick; Busse, James S; Bussan, Alvin J; Jiang, Jiming; Bethke, Paul C

    2014-01-01

    Sugar-end defect is a tuber quality disorder and persistent problem for the French fry processing industry that causes unacceptable darkening of one end of French fries. This defect appears when environmental stress during tuber growth increases post-harvest vacuolar acid invertase activity at one end of the tuber. Reducing sugars produced by invertase form dark-colored Maillard reaction products during frying. Acrylamide is another Maillard reaction product formed from reducing sugars and acrylamide consumption has raised health concerns worldwide. Vacuolar invertase gene (VInv) expression was suppressed in cultivars Russet Burbank and Ranger Russet using RNA interference to determine if this approach could control sugar-end defect formation. Acid invertase activity and reducing sugar content decreased at both ends of tubers. Sugar-end defects and acrylamide in fried potato strips were strongly reduced in multiple transgenic potato lines. Thus vacuolar invertase silencing can minimize a long-standing French fry quality problem while providing consumers with attractive products that reduce health concerns related to dietary acrylamide. PMID:24695527

  4. COMPLEMENTATION OF THE AMYLOSE-FREE STARCH MUTANT OF POTATO (SOLANUM-TUBEROSUM) BY THE GENE ENCODING GRANULE-BOUND STARCH SYNTHASE

    NARCIS (Netherlands)

    VANDERLEIJ, FR; VISSER, RGF; OOSTERHAVEN, K; VANDERKOP, DAM; JACOBSEN, E; FEENSTRA, WJ

    1991-01-01

    Agrobacterium rhizogenes-mediated introduction of the wild-type allele of the gene encoding granule-bound starch synthase (GBSS) into the amylose-free starch mutant amf of potato leads to restoration of GBSS activity and amylose synthesis, which demonstrates that Amf is the structural gene for GBSS.

  5. Isolation and characterization of a β-propeller gene containing phosphobacterium Bacillus subtilis strain KPS-11 for growth promotion of potato (Solanum tuberosum L.)

    NARCIS (Netherlands)

    Hanif, Muhammad Kashif; Hameed, Sohail; Imran, Asma; Naqqash, Tahir; Shahid, Muhammad; Van Elsas, Jan D

    2015-01-01

    Phosphate-solubilizing and phytate-mineralizing bacteria collectively termed as phosphobacteria provide a sustainable approach for managing P-deficiency in agricultural soils by supplying inexpensive phosphate to plants. A phosphobacterium Bacillus subtilis strain KPS-11 (Genbank accession no. KP006

  6. Evaluación de diferentes combinaciones fitohormonales en la regeneración de Solanum tuberosum (Solanaceae Var. Pastusa Suprema a partir de explantes internodales

    Directory of Open Access Journals (Sweden)

    Jenny Paola Jiménez Barreto

    2009-10-01

    The presence of NAA was seen to be essential in explants’ callogenic and regenerative response. Adding 3.0 mg/L ZR, 0.02 mg/L NAA and 1.0mg/L GA3 to a basic M-S medium was an appropriate hormonal combination for inducing indirect organogenesis in the Pastusa Suprema potato variety; it produced more than 90% callus genesis and plant regeneration and an average of six regenerants per explant. Key words: zeatin riboside; naphthalene acetic acid; gibberellic acid; indirect organogenesis.

  7. Impact of foliar application of seaweed extract on growth, yield and quality of potato (Solanum tuberosum L.)

    OpenAIRE

    Muhammad Wasim Haider, Chaudhary Muhammad Ayyub, Muhammad Aslam Pervez, Habat Ullah Asad, Abdul Manan, Syed Ali Raza and Irfan Ashraf

    2012-01-01

    A field trial was carried out in 2010 to investigate the effect of foliar application of seaweed extract “Primo” as an organic biostimulant on potato cv. ‘Sante’. Foliar application of seaweed extract was carried out at different growth stages of the crop (i.e. 30 days; 45 days; 60 days; 30 and 45 days; 30 and 60 days; 45 and 60 days; 30, 45 and 60 days after sowing). Control plants were sprayed with water without seaweed extract. A significant improvement in growth, yield and tuber quality o...

  8. Vacuolar invertase gene silencing in potato (Solanum tuberosum L. improves processing quality by decreasing the frequency of sugar-end defects.

    Directory of Open Access Journals (Sweden)

    Xiaobiao Zhu

    Full Text Available Sugar-end defect is a tuber quality disorder and persistent problem for the French fry processing industry that causes unacceptable darkening of one end of French fries. This defect appears when environmental stress during tuber growth increases post-harvest vacuolar acid invertase activity at one end of the tuber. Reducing sugars produced by invertase form dark-colored Maillard reaction products during frying. Acrylamide is another Maillard reaction product formed from reducing sugars and acrylamide consumption has raised health concerns worldwide. Vacuolar invertase gene (VInv expression was suppressed in cultivars Russet Burbank and Ranger Russet using RNA interference to determine if this approach could control sugar-end defect formation. Acid invertase activity and reducing sugar content decreased at both ends of tubers. Sugar-end defects and acrylamide in fried potato strips were strongly reduced in multiple transgenic potato lines. Thus vacuolar invertase silencing can minimize a long-standing French fry quality problem while providing consumers with attractive products that reduce health concerns related to dietary acrylamide.

  9. Rheological study of mixed flour: wheat (Triticum vulgare, barley (Hordeum vulgare and potato (Solanum tuberosum for use in the preparation of bread

    Directory of Open Access Journals (Sweden)

    Galo Sandoval

    2012-06-01

    Full Text Available With wheat flour imported and domestic wheat cereals produced in the country, and the potato tuber, a rheological study was performed to determine the most suitable proportions of substitution of wheat flour imported with the latter and its feasibility for making bread. We worked in mixtures of flour, wheat CWRS # 1 (red spring wheat in western Canada Cañicapa barley flour, wheat and potato Cojitambo Gabriela, from Ecuadorian cultures in proportions of 10, 20 and 30% (p / p. Masses from mixtures of flours were analyzed on a Brabender Farinograph, in order to determine the water absorption, development time, stability and rate of tolerance with a view to selecting the flour blends that have a behavior similar to CWRS wheat flour # 1. The best mixtures found were: wheat flour # 1 CWRS replaced with 10, 20 and 30% barley flour Cañicapa, and the mixture of wheat flour # 1 CWRS wheat flour in Cojitambo 30%. These flour mixes selected were also subjected to rheological analysis of their masses using a computer Mixolab. The breads made from flours selected were evaluated in a sensory panel. The breads more accepted by consumers were those containing 20 and 30% barley, followed by the group of those made with imported wheat with 30% wheat Cojitambo, and containing 10% of barley flour.

  10. Isolation of a Plasmalemma Aquaporin Encoding Gene StPIP1 from Solanum tuberosum L. and Its Expression in Transgenic Tobacco

    Institute of Scientific and Technical Information of China (English)

    WU Wang-ze; PENG Xiao-li; WANG Di

    2009-01-01

    Aquaporin (AQP) belongs to a highly conserved group of membrane proteins considered as major intrinsic proteins, which facilitate water transport across biological membranes. The discovery of AQPs in plants has resulted in a paradigm shift in the understanding of plant-water relations, however, the potential relationship between the role of aquaporins in regulating plant water balance and drought tolerance still remains elusive. In this study, the gene encoding potato AQP cDNA, StPIP1 (GenBank accession no. DQ999080), was cloned from the leaf of potato cultivar Gannongshu 2 by reverse transcription-PCR (RT-PCR). Sequence alignment was made by BLASTn in GenBank, the phylogenetic analysis was conducted using PHYLIPWY, the 3D structure was predicted in Swiss-Model server. Subcellular localization of StPIP1 was performed by constructing CaMV35S-StPIP1-GFP and rd29A-StPIP1-GFP fusion proteins and transient expression in onion epidermis. To understand StPIP1 physiological functions in potato under various stress conditions, the StPIP1 gene in a reverse orientation was transformed into tobacco driven by the Cauliflower mosaic virus (CMV) 35S promoter. The expression levels of transgenic and wild-type plants were assessed under various abiotic stress conditions using semi-quantitative RT-PCR, and the morphological and physiological responses of transgenic plants to different stress conditions were investigated. The expression of StPIP1 mRNA decreased in transgenic plants under non-stress and stress conditions, however, the reduction was more severer under drought stress. In both non-stress and stress conditions, StPIP1 was expressed predominantly in root. The morphological and physiological investigation showed no significant differences in growth rate, germination rate, and root fresh weight (FW) between transgenic and wild-type plants when grown under favorable conditions. In contrast, under drought stress, the reduction in StPIP1 expression leads to a delay in seed germination and seedling growth, accelerated seedling wilt, and leaf morphological abnormity. Under "enough" water conditions (i.e., water culture), the aerial parts of anti-sense plants showed no differences. However, for the aerial parts to accumulate the same amount of biomass, transgenie plants needed about 3 times more abundant root system to transport water for plant growth than wild-type plants. Morphological investigation showed that the reduction in StPIP1 expression increased the root system in transgenic plants under drought stress. As a result, the increase of root mass might compensate the reduced cellular water permeability in order to ensure a sufficient water supply for the plant. Results demonstrated that StPIP1 plays an important role for water transportation in potato, especially under drought stress conditions. The reduced expression of StPIP1 decreases the cellular water transport and influences the expression of endogenous AQPs genes and thereby, has impacts on seed germination, seedling growth, and stress responses of potato to drought conditions.

  11. Development and Evaluation of Codon-modified cry V Constructs in Cultivated Potato (Solanum tuberosum L. ) for Control of Potato Tuber Moth

    Institute of Scientific and Technical Information of China (English)

    Guan Hongju; Li Wenbin; Douches David; Lu Cuihua

    2007-01-01

    A cryV gene, specifically toxic to Lepidoptera and Coleoptera, was incorporated into binary vectors with different promoters and the presence or absence of the β -glucuronidase (gus) reporter gene. These constructs were integrated into potato cv. Spunta by Agrobacterium tumefaciens-mediated transformation. Highest expression of cryV gene, determined by mRNA levels and insect mortality, was obtained using the CaMV 35S promoter without the gus gene configuration.Detached leaf and tuber bioassays showed a mortality rate of up to 83% and 100%, respectively, for potato tuber moth (Phthorimaea operculella Zeller) in the transgenic lines. Our results demonstrated that the presence of the gus gene negatively affects the expression level of the cryV gene. Bt expression was also facilitated by using the (ocs)3 mas super promoter, whereas the Bt expression regulated by the patatin promoter (tuber-specific) was too low to have any effect upon the mortality of potato tuber moth. These results represent significant improvement in the level of host plant resistance for the control of potato tuber moth via Bt transgenes.

  12. Efecto de la temperatura sobre el crecimiento de plantas de papa (Solanum tuberosum L., variedades atlantic y 1625 frito lay, crecidas in vitro

    Directory of Open Access Journals (Sweden)

    Rafael Orozco

    2016-03-01

    Full Text Available En esta investigación, se estudio el efecto que causa sobre el crecimiento vegetativo de dos variedades de papa (1625 Frito Lay y Atlantic, el aumento de la temperatura en condiciones in vitro, para lo cual se usaron dos valores de temperatura (30/26 °C y 27/25 °C dia/noche. El medio de cultivo utilizado fue el MS (1962 de laboratorios Gibco, el cual se usó a razón de 17 g/L y se suplementó con vitaminas y aminoácidos del mismo medio. En ambas variedades la alta temperatura (30/26 °C causó una disminución en el diámetro del tallo y en el número de nudos por planta, así como un incremento en la longitud internodal y en la altura de la planta. Por otro lado, la alta temperatura disminuyó significativamente (P < 0,05 la longitud de la raíz en la variedad Frito Lay mientras que la variedad Atlantic mostró un incremento no significativo.

  13. SELECCIÓN In Vitro DE FORMAS TOLERANTES DE PAPA (Solanum tuberosum, L. A FILTRADOS DE Phytophthora infestans (Mont. de Bary

    Directory of Open Access Journals (Sweden)

    María M. Hernández

    2003-01-01

    Full Text Available Se realizaron una serie de experimentos para determinar la acción patogénica de filtrados de P. infestans (Mont. de Bary, sobre diferentes órganos de papa y verificar si éstos podían ser empleados en la selección in vitro de formas tolerantes a este patógeno. Se emplearon hojas desprendidas, así como el polen de clones con diferente respuesta al patógeno en el campo, los cuales fueron sometidos a la acción del filtrado y se encontró que éste poseía una acción hospedero específica, o sea, los clones respondieron a su acción en dependencia de su resistencia al patógeno, por lo cual este podía ser usado para la selección de formas resistentes. Los filtrados fueron empleados para seleccionar brotes de callos sometidos a su acción y estos fueron llevados finalmente al campo para evaluar su tolerancia frente al patógeno, incluyéndose la evaluación de la incidencia de Alternaria solani, que es la más importante después de Phytophthora infestans, para comprobar si los somaclones obtenidos presentaban un nivel de tolerancia superior al donante en sentido general. El donante empleado fue la var Desirée, que presenta buenas características en cuanto a rendimiento y calidad de los tubérculos, pero es susceptible al tizón causado por P. infestans. Los resultados demostraron que los clones superaron al donante de manera general, no sólo en cuanto a resistencia a ambos patógenos, sino en los principales caracteres agronómicos y el rendimiento, lo cual abre grandes perspectivas para el empleo de estas técnicas en el mejoramiento genético del cultivo.

  14. Genetic characterisation of agronomic and morphological traits and the development of DNA markers associated with total glycoalkaloid content in the tubers of tetraploid potato (Solanum tuberosum L.)

    NARCIS (Netherlands)

    Dam, van J.

    2002-01-01

    Glycoalkaloids are secondary metabolites that are characterised by an undesirable taste, and which are known to be toxic when consumed in large quantities. Some wild potato germplasms that are used for the introgression of genes

  15. COMPETITION BETWEEN FOUR GERMAN POTATO VARIETIES COMPETIÇÃO ENTRE QUATRO VARIEDADES ALEMÃS DE BATATINHA

    Directory of Open Access Journals (Sweden)

    Nelson Salim Abbud

    2007-09-01

    Full Text Available

    A competition experiment was performed at the Federal University of Goiás, among four German varieties of potato. The results show significant advantages of the varieties Olympia and Maritta over Delta-A and Anco.

    A variedade de batata alemã Delta-A, muito popular no sul de Goiás, foi comparada com as variedades Anco, Maritta e OIympia, também de procedência alemã. Olympia e Maritta, apresentaram-se significativamente superiores às variedades Delta-A e Anco quanto à produção total.

    PALAVRAS-CHAVE: Solanum tuberosum; ensaio nacional de batata; batatinha.

  16. Výskyt hybridizace mezi lilkem černým (Solanum nigrum) a lilkem leskloplodým (Solanum physalifolium)

    OpenAIRE

    Dobešová, Tereza

    2012-01-01

    Solanum nigrum is an important weed species, which occurrs mainly on fertile soils in wide row crops as sugar beet, maize, potatoes, but also in vegetable stands. Solanum physafilium is a new invasive weed species, which grows as a weed among root-crops and vegetable. Both mentioned species are annual weeds. In the field some plants with inter-medial characteristics were indicated, on which is possible to expect them to be products of hybridization between Solanum physafilium and common Solan...

  17. Postharvest Quality and Physiological Behavior of Sweet Potato (Ipomoea batatas Lam.) Leaf Stalks Under Three Temperatures

    Institute of Scientific and Technical Information of China (English)

    PENG Yan; XU Yong-quan; DUAN Dao-fu; MAO Lin-chun

    2009-01-01

    Sweet potato (lpomoea batatas Lam.) leaf stalks were cut into 20 cm length, and stored at 20, 6, and 2℃. The respiration rate, ethylene production, ascorbic acid, free amino acid, total chlorophyll content, freshness, and shelf life were determined during storage to investigate the effect of storage temperatures on the quality and physiological responses in sweet potato leaf stalks. Wound responses were observed as high respiration rate and ethylene production immediately after cutting. Sweet potato leaf stalks were found to be sensitive to chilling injury manifested as browning and water-soaking on the surface at 2℃. In contrast, sweet potato leaf stalks were susceptible to senescence, exhibited by etiolating and yellowing, at 20℃. Loss in weight and chlorophyll was minimized under low temperatures. High temperatures also caused the accumulation of amino acids with a significant loss of ascorbic acid and chlorophyll. Sweet potato leaf stalks had a storage life of 16 days at 6℃, 8 days at 2℃, and 6 days at 20℃, respectively.

  18. Coronatine Induces an Accumulation of Anthocyanin and Starch in Purple-fleshed Sweetpotato (Ipomoea batatas Lam.)

    Institute of Scientific and Technical Information of China (English)

    Baoqing WANG; Beitao XIE; Haiyan ZHANG; Liming ZHANG; Qingmei WANG

    2013-01-01

    [Objective] The objective of this research was to examine the effects of COR on anthocyanin and starch content in storage roots of two PFS genotypes, and to explore the relationships between anthocyanin synthesis and starch accumula-tion. [Method] A field experiment was carried out to determine the changes in yield components, yield, contents of anthocyanin and starch, activities of phenylalanine ammonia-lyase (PAL) and adenosine 5-diphosphate glucose pyrophosphorylase (AG-Pase) in two genotypes of PFS (Ipomoea batatas L., var. ‘Ayamurasaki’ and‘Jishu18’). [Result] The application of COR significantly increased starch and antho-cyanin content in storage roots of Jishu18 across developmental stages by inducing the activities of PAL and AGPase, and final y enhanced yield by promoting fresh weight of storage roots. Ayamurasaki was insensitive to treatment with COR al-though its PAL activity temporal y increased. The starch and anthocyanin content of Aya, and the anthocyanin content of Jishu18 increased progressively across devel-opmental stages with or without COR application, but the starch content of Jishu18 increased initial y, then decreased before increasing again without application of COR. Treatment with COR reduced downward trend of starch accumulation in Jishu18. Thus, the effect of COR on accumulation of anthocyanin and starch in storage roots of PFS differs according to genotypes. [Conclusion] The application of 0.05 μmol/L COR may increase starch and anthocyanin content in PFS genotypes with lower starch and anthocyanin content in storage roots.

  19. Estabilidade de farinha de batata-doce biofortificada Stability of biofortified sweet potato flour

    Directory of Open Access Journals (Sweden)

    Rosa Maria Vercelino Alves

    2012-03-01

    Full Text Available A farinha de batata-doce, quando elaborada a partir de raízes com alto teor de β-caroteno, é fonte de pró-vitamina A, que apresenta efeitos benéficos na saúde humana. Visando contribuir com o suprimento de vitamina A na alimentação de populações carentes, a EMBRAPA tem selecionado e melhorado culturas via biofortificação, como da batata-doce, e pesquisado o desenvolvimento de produtos com esta matéria-prima biofortificada. Complementando esses desenvolvimentos, o objetivo deste estudo foi comparar o efeito do tipo de embalagem e do sistema de acondicionamento do produto na preservação de carotenoides de farinha de batata-doce biofortificada. A farinha foi acondicionada com e sem vácuo, em embalagens com diferentes barreiras ao oxigênio, vapor d'água e luz (PET/Al/PEBD, PETmet/PEBD, PEBD/PA/PEBD e PEBD e estocada a 25 °C/75%UR com e sem exposição à luz por um ano. Perdas de 50% de carotenoides totais e β-caroteno ocorreram na farinha em PETmet/PEBD e PEBD sem vácuo, aos 50 dias de estocagem, em razão do teor de oxigênio residual presente no espaço livre e também da alta permeação de oxigênio pelo material no caso do PEBD, no qual a composição do ar foi mantida durante todo o estudo. Perdas de 50% de carotenoides ocorreram aos 90 dias com ou sem exposição à luz no produto em PEBD/PA/PEBD com vácuo, indicando que a reação de oxidação de carotenoides sob luz se restringe apenas à farinha em contato com a superfície da embalagem. Sob vácuo e em PETmet/PEBD, os teores de carotenoides na farinha diminuíram ligeiramente e foram mantidos no PET/Al/PEBD, até 360 dias de estocagem. Os resultados indicaram que a redução do teor de oxigênio no espaço livre da embalagem por meio da aplicação de vácuo aliada ao uso de materiais de embalagem com barreira ao oxigênio da ordem de grandeza de PET com metalização barreira são os fatores mais importantes na preservação de carotenoides em farinha de batata

  20. A proteomic analysis of storage stress responses in Ipomoea batatas (L.) Lam. tuberous root.

    Science.gov (United States)

    Jiang, Yusong; Chen, Cheng; Tao, Xiang; Wang, Jianxi; Zhang, Yizheng

    2012-08-01

    During post-harvest storage, tuberous roots of sweet potato (Ipomoea batatas L. Lam.) usually undergo a biotic and abiotic stress influencing protein expression pattern and substance contents. This research compared the change of total proteins and carbohydrate content in tuberous roots of sweet potato during the storage period. The result of the two-dimensional electrophoresis analysis demonstrated that there were 25 differentially expressed proteins between day 0 and day 75 during the storage. Among these proteins, 11 proteins were down-regulated and the other 14 were up-regulated. The results from MALDI-TOF-TOF/MS analyses and mascot database searching showed that 11 of the 25 differentially expressed proteins were identified as store-stress regulated proteins. It was also found that the proteins involved in the energy metabolism and the stress-response were drastically up-regulated, whereas those in biomacromolecule synthesis were markedly down-regulated. Meanwhile, under the experimental conditions, the content of the starch and the cellulose was decreased by more than a quarter and the amylase activity was increased moderately. PMID:22547271

  1. Microbial deterioration of white variety sweet potato (Ipomoea batatas under different storage structures

    Directory of Open Access Journals (Sweden)

    Wisdom Amoa-Awua

    2010-01-01

    Full Text Available Post-harvest protection of white variety sweet potato (Ipomoea batatas has been dealt with rarely in the past, although it is comparatively easy to grow and has high consumer acceptability in Ghana. Microbial deterioration of sweet potato roots stored in three different storage structures was studied. The sweet potato roots initially cured for 7 and 14 days were stored in traditional, pit, and clamp storage structures for a maximum of 28 days. For the 7 days-cured sweet potato roots, the bac­teria population in the three different storage structures increased by 1.2-2.3 log cfu/g whereas for the 14 days-cured roots, the bacteria population was 0.1-1.0 log cfu/g within 28 days of storage. The fungal population in the 14 days-cured sweet potato roots was higher than in the 7 days-cured sweet potato roots by 0.6-1.6 log cfu/g for 28 days of storage. For both the 7 and 14 days-cured sweet potato roots, the sweet potato roots stored for 28 days in the three different storage structures had a higher microbial count compared to the sweet potato roots stored for 14 days. Aspergillus flavus was the most dominant fungal species occurring in all of the three different storage structures followed by A. niger, Rhizopus stolonifer, Tricho-derma viride, Fusarium oxysporum, Penicillium digitatum, Cladosporium herbarum,and Aspergillus ochraceus, in that order.

  2. Phytochemical screening, antioxidant, and antimicrobial activities of the crude leaves′ extract from Ipomoea batatas (L. Lam

    Directory of Open Access Journals (Sweden)

    Márcia Thaís Pochapski

    2011-01-01

    Full Text Available Background: Ipomoea batatas (L. Lam., popularly known as sweet potato (SP, has played an important role as an energy and a phytochemical source in human nutrition and animal feeding. Ethnopharmacological data show that SP leaves have been effectively used in herbal medicine to treat inflammatory and/or infectious oral diseases in Brazil. The aim of this research was to evaluate the phytochemical, antioxidant, and antimicrobial activities of the crude leaves′ extract of SP leaves. Materials and Methods: The screening was performed for triterpenes/steroids, alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, and phenolic acids. The color intensity or the precipitate formation was used as analytical responses to these tests. The total antioxidant capacity was evaluated by the phosphomolybdenum complex method. Antimicrobial activity was made by agar disk and agar well diffusion tests. Results: The phytochemical screening showed positive results for triterpenes/steroids, alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, and phenolic acids. Total contents of 345.65, 328.44, and 662.02 mg were respectively obtained for alkaloids, anthraquinones, and phenolic compounds in 100 g of the dry sample. The total antioxidant capacity was 42.94% as compared to ascorbic acid. For antimicrobial studies, no concentration of the SP freeze dried extract was able to inhibit the growth of Streptococcus mutans, S. mitis, Staphylococcus aureus, and Candida albicans in both agar disk and agar well diffusion tests. Conclusions: SP leaves demonstrated the presence of secondary metabolites with potential biological activities. No antimicrobial activity was observed.

  3. Hydrogen Sulfide Promotes Root Organogenesis in Ipomoea batatas, Salix matsudana and Glycine max

    Institute of Scientific and Technical Information of China (English)

    Hua Zhang; Jun Tang; Xiao-Ping Liu; Yun Wang; Wei Yu; Wei-Yan Peng; Fang Fang; Dai-Fu Ma; Zhao-Jun Wei; Lan-Ying Hu

    2009-01-01

    In this report, we demonstrate that sodium hydrosulfide (NaHS), a hydrogen sulfide (H2S) donor, promoted adventitious root formation mediated by auxin and nitric oxide (NO). Application of the H2S donor to seedling cuttings of sweet potato (Ipomoea batatas L.) promoted the number and length of adventltious roots in a dose-dependent manner. It was also verified that H2S or HS- rather than other sulfur-containing components derived from NariS could be attributed to the stimulation of adventitious root formation. A rapid Increase In endogenous H2S, indole acetic acid (IAA) and NO were sequentially observed in shoot tips of sweet potato seedlings treated with HallS. Further investigation showed that HzS-mediated root formation was alleviated by N-l-naphthylphthalamic acid (NPA), an IAA transport inhibitor, and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), an NO scavenger. Similar phenomena in H2S donor-dependent root organogenesis were observed in both excised willow (Sallx matsudana var. tortuosa Vilm) shoots and soybean (Glycine max L.) seedlings. These results indicated that the process of H2S-induced adventitious root formation was likely mediated by IAA and NO, and that H2S acts upstream of IAA and NO signal transduction pathways.

  4. 7 CFR 301.86-2 - Regulated articles.

    Science.gov (United States)

    2010-01-01

    ... following pale cyst nematode host crops: Eggplant (Solanum melongena L.) Pepper (Capsicum spp.) Potato (Solanum tuberosum L.) Tomatillo (Physalis philadelphica) Tomato (Lycopersicon esculentum L.) (c)...

  5. Adubação NPK e tamanho da batata-semente no crescimento, produtividade e rentabilidade de plantas de batata NPK fertilization and potato tuber seed size on growth, yield and profitability of potato plants

    Directory of Open Access Journals (Sweden)

    Luiz Raphael de M Queiroz

    2013-03-01

    Full Text Available A cultura da batata demanda alto investimento para a sua implantação. Dentre os insumos responsáveis pelo alto custo da lavoura, destacam-se os fertilizantes e a semente. O objetivo deste trabalho foi identificar o efeito de doses de fertilizantes e tamanhos de batata-sementes no crescimento, produtividade e rentabilidade da cultura da batata. O experimento foi conduzido em uma lavoura comercial de batata em Guarapuava-PR, a partir de dezembro de 2010. Os tratamentos foram constituídos de 0, 2, 4 e 6 t ha-1 do fertilizante mineral 4-14-08 (parcela e batatas-semente do tipo I e III (subparcela, arranjados em esquema de parcela subdividida, em blocos ao acaso, com três repetições. Foram avaliados o comprimento da haste principal, número de hastes, índice de área foliar, massa fresca e número de tubérculos, aos 24, 41 e 57 dias após a emergência, além de quantificar a produtividade comercial e o número de tubérculos no período da colheita. Calculou-se também a máxima eficiência técnica e econômica das doses de fertilizantes. Não houve interação significativa entre doses de fertilizantes e tamanhos de batata-semente em nenhuma característica analisada. As doses de fertilizantes influenciaram todas as características analisadas, sendo que, de modo geral, as maiores doses resultaram em maiores valores das características. Entretanto, as doses de fertilizantes influenciaram com menor intensidade o número de hastes, que foi a característica mais influenciada pelo tamanho da batata-semente, sendo maior em plantas oriundas de sementes do tipo I. O tamanho da batata-semente não influenciou a produtividade de tubérculos. Observou-se aumento da produtividade de tubérculos com o aumento da dose de fertilizante até 4,2 t ha-1, porém a máxima eficiência econômica foi atingida com 3,5 t ha-1 de fertilizante. Conclui-se, portanto, que a utilização da dose de 3,5 t ha-1 e batatas-semente do tipo III resultaram na melhor op

  6. Management of Solanum elaeagnifolium in the Mediterranean Basin

    Science.gov (United States)

    Solanum elaeagnifolium Cav. (silverleaf nightshade, SOLEL) is a prominent invasive alien weed in many countries of the Mediterranean Basin since its introduction in the mid-20th century, originating from the southwestern United States and northern Mexico. It reproduces vegetatively and by seeds that...

  7. Core collections of potato (Solanum) species native to the USA

    Science.gov (United States)

    Potato has two wild relatives native to the USA, Solanum jamesii (jam) and S. fendleri (fen). Core collections are a useful tool for genebanks, identifying a ranked minimum number of samples that together encompass most of the total diversity. With diversity measured as presence of AFLP bands, core ...

  8. IPOMOEA BATATAS SYRUP DECREASE MALONDIALDEHYDE AND INCREASE NITROUS OXIDE PLASMA LEVELS AMONGST MODERATE SMOKER WORKERS AT DENPASAR

    Directory of Open Access Journals (Sweden)

    I Dewa Ayu Intan Dwi-Primayanti

    2012-09-01

    Full Text Available Objective: Cells and tissues are continuously damaged by reactive oxygen species. Cigarette smoke is one of an exogenous source of free radical containing more than 4000 chemical compounds, that triggering the formation of free radicals related to diseases and aging process. Anthocyanins are potent antioxidants that are widely distributed in fruit, vegetables, red wines and Ipomea batatas. The aim of this study was to determine the effect of Ipomea batatas as a source of antioxidants in decreasing levels of alondialdehyde (MDA and increasing of Nitrous oxide (nitrite/nitrate/NOxplasma in moderate smokers of workers at Denpasar.Method: This was an experimental study with a pretest-posttest control groups design. There are 33 moderate smokers who were divided into three groups, control group (placebo, treatment group with 15 ml purple sweet potato syrup (P1 and 30 ml (P2, for 14 days. All groups were performed the laboratory examinations for MDA and NOx plasma before and after the treatment.Results: This study showed that there were significant differences (p<0.05 both in MDA and NOx plasma levels in the control group, P1 and P2. The decrease of MDAlevels on P1 was 35.39% and on P2 was 49.87%. The increase of NOx plasma levels was 7.78% for P1 and 14.68% for P2.Conclusion: From this study, it can be concluded that Ipomea batatas syrup contains of 8mg/mL anthocyanins, probably play a role in reducing the free radical and thus reducingthe risk of disease and slowing the aging process.

  9. CONTROLE QUÍMICO DE Rhizoctonia solani Kuhn EM BATATA SEMENTE CHEMICAL CONTROL OF Rhizoctonia solani Kuhn IN SEED POTATO

    OpenAIRE

    Wilson Ferreira de Oliveira; Cláudio Aparecido da Silveira

    2007-01-01

    Os fungicidas benomyl (200 g.i.a./100 kg sementes), iprodione (200 g.i.a./100 kg sementes) e pencycuron (62,5 g.i.a./100 kg de sementes) foram testados, em condições de telado, no controle de Rhizoctonia solani Kuhn., em batata semente da cultivar Desirée. A avaliação da percentagem de emergência damping-off aos 25 dias após o plantio, demonstrou que os fungicidas pencycuron e iprodione foram os...

  10. Yam (Dioscorea batatas) Root and Bark Extracts Stimulate Osteoblast Mineralization by Increasing Ca and P Accumulation and Alkaline Phosphatase Activity

    OpenAIRE

    Kim, Suji; Shin, Mee-Young; Son, Kun-Ho; Sohn, Ho-Yong; Lim, Jae-Hwan; Lee, Jong-Hwa; Kwun, In-Sook

    2014-01-01

    Yam (Dioscorea batatas) is widely consumed as functional food for health promotion mainly in East Asia countries. We assessed whether yam root (tuber) or bark (peel) extracts stimulated the activity of osteoblasts for osteogenesis. MC3T3-E1 cells (mouse osteoblasts) were treated with yam root extracts (water or methanol) (study I) or bark extracts (water or hexane) (study II) within 0~10 μg/mL during the periods of osteoblast proliferation (5~10 day), matrix maturation (11~15 day) and mineral...

  11. Effect of gamma-Irradiation on the Volatile Organic Compounds from Leek (Allium tuberosum R.)

    International Nuclear Information System (INIS)

    Volatile compounds of γ-irradiated leeks (Aillum tuberosum R.) were isolated by simultaneous steam distillation and extraction method (SDE) and analyzed by gas chromatography/mass spectrometry (GC/MS) along with the non-irradiated sample. Leeks were irradiated at dose of 20 kGy and non-irradiated samples were considered as control sample. 56 and 54 compounds were identified in non-irradiated and irradiated samples, respectively. These components included the functional groups; alcohols, aldehydes, esters, ketones, N- and S-containing compounds

  12. Quantification of vitamin D3 and its hydroxylated metabolites in waxy leaf nightshade (Solanum glaucophyllum Desf.), tomato (Solanum lycopersicum L.) and bell pepper (Capsicum annuum L.)

    DEFF Research Database (Denmark)

    Jäpelt, Rie Bak; Silvestro, Daniele; Smedsgaard, Jørn;

    2013-01-01

    Changes in vitamin D3 and its metabolites were investigated following UVB- and heat-treatment in the leaves of Solanum glaucophyllum Desf., Solanum lycopersicum L. and Capsicum annuum L. The analytical method used was a sensitive and selective liquid chromatography electrospray ionisation tandem...

  13. Life history parameters of the biocontrol agent Gratiana spadicea (Chrysomelidae), reared on the natural host plant Solanum sisymbriifolium and the non-target crop Solanum melongena (Solanaceae)

    Science.gov (United States)

    Gratiana spadicea (Klug), a leaf-feeding tortoise beetle native to South America, was released in South Africa for the biological control of Solanum sisymbriifolium Lamarck (wild tomato), despite its ability to develop on cultivated eggplant (Solanum melongena L.) during laboratory host-specificity ...

  14. Qualidade físico-química e de fritura de tubérculos de cultivares de batata na safra de inverno Physicochemical and frying quality of potato cultivars in winter season

    OpenAIRE

    Adalton M Fernandes; Rogério P Soratto; Regina Marta Evangelista; Israel Nardin

    2010-01-01

    Com o aumento do consumo de batata processada na forma de fritura, torna-se essencial a identificação dos parâmetros de qualidade dos tubérculos de batata. Objetivou-se avaliar a composição físico-química e qualidade de fritura de tubérculos de cultivares de batata, produzidos na safra de inverno. Os tubérculos foram provenientes de experimento de campo conduzido durante a safra de inverno de 2008, no município de Itaí-SP. o delineamento experimental utilizado foi blocos casualizados, com qua...

  15. HPLC-DAD Analysis and In-Vitro Property of Polyphenols Extracts from (Solanum Aethiopium) Fruits on α -Amylase, α -Glucosidase and Angiotensin - 1- Converting Enzyme Activities

    OpenAIRE

    Nwanna, E. E; Ibukun, E. O; G. Oboh; Ademosun, A. O.; A.A. BOLIGON; Athayde, M.

    2014-01-01

    AIM: Garden egg (Solanum aethiopium) is an edible fruits vegetable with  different species.This study investigated characterisation and the effect of the phenolics extracts from S. aethiopium species with enzymes linked with type -2-diabetes (α-amylase and α-glucosidase) and hypertension [Angiotensin-1-converting enzyme (ACE)]. METHODS: Fresh samples of the 5 species of the garden egg namely, [Solanum gilo (PW), Solanum torvum (TWS), Solanum kumba (PGR), Solanum incanum (GSB), and Solanum ind...

  16. Controle de plantas daninhas na cultura da batata Potato weed control by application of herbicides

    Directory of Open Access Journals (Sweden)

    Jeferson Zagonel

    1999-03-01

    Full Text Available O experimento foi conduzido na Fazenda Escola da UEPG, em Ponta Grossa-PR, em Latossolo Vermelho Escuro de textura média argilosa, com o objetivo de verificar a praticabilidade e a eficiência agronômica dos herbicidas metolachlor e metribuzin + metolachlor no controle de plantas daninhas na cultura da batata. O delineamento experimental foi de blocos ao acaso com nove tratamentos e quatro repetições, quais sejam: metolachlor (1,92; 2,88 e 3,84 kg i.a./ha; metribuzin + metolachlor (0,24 + 1,68; 0,36 + 2,52 e 0,48 + 3,36 kg i.a./ha; metribuzin (0,48 kg i.a./ha; testemunha capinada e testemunha sem capina. O plantio foi realizado em outubro/95 utilizando-se a cultivar Elvira, no espaçamento 0,70 x 0,35 m. As plantas daninhas predominantes foram Brachiaria plantaginea (capim-papuã, Digitaria horizontalis (capim-milhã e Galinsoga parviflora (fazendeiro. As avaliações foram efetuadas aos 15, 30 e 45 dias após a aplicação dos tratamentos onde verificou-se que foram eficientes os seguintes herbicidas: metolachlor no controle sobre capim-papuã e capim-milhã nas doses de 2,88 e 3,84 kg i.a./ha; a mistura metribuzin + metolachlor no controle sobre capim-papuã e capim-milhã nas doses 0,36 + 2,52 e 0,48 + 3,36 kg i.a./ha; metolachlor (1,92; 2,88 e 3,84 kg i.a./ha e metribuzin + metolachlor (0,24 + 1,68; 0,36 + 2,52 e 0,48 + 3,36 kg i.a./ha no controle sobre fazendeiro. Não foram observados efeitos fitotóxicos dos produtos sobre as plantas de batata.The presented field trial was conducted at the Universidade Estadual de Ponta Grossa, Paraná State, Brazil, on a Dark Red Latossoil, to evaluate the availability and efficiency of herbicides metolachlor and metribuzin + metolachlor in the control of weeds in the potato crop. The experiment was laid out in a randomized block design with nine treatments and four replications as follows: metolachlor (1.92; 2.88 and 3.84 kg a.i/ha; metribuzin + metolachlor (0.24 + 1.68; 0.36 + 2.52 and 0.48 + 3

  17. Phenetic analysis of medicinally important species of the genus solanum from Pakistan

    International Nuclear Information System (INIS)

    Solanum is one of the largest and hyper diverse genera of the family Solanaceae. In Pakistan Solanum is represented by 15 species, of which 11 species have the medicinal properties. Taxonomically this is a complex genus because of the presence of number of hybrid and controversial taxonomic status of S. nigrum complex. In the present study numerical techniques were utilized to evaluate the taxonomic status of the genus Solanum. Cluster analysis was employed to work out the relationship among the taxa of the genus Solanum. The Euclidean distance measured similarity matrix and a dendrogram was constructed by using the complete linkage method. This analysis showed that all the species of genus Solanum can easily be divided into two groups at hundred percentage linkage distance. Co-relation of quantitative characters showed that floral characters had highly significant relationship with the stem characters, these characters plays a significant role in the identification of the species of the genus Solanum. (author)

  18. Two rubisco activase genes from ipomoea batatas have different roles in photosynthesis of arabidopsis

    International Nuclear Information System (INIS)

    Rubisco activase (RCA) that functions as a molecular chaperone regulates the activity of the Calvin-Benson cycle via regulation of the Rubisco activity. In plants such as Arabidopsis thaliana, Spinacia oleracea, and Oryza sativa, there are two RCA isoforms from two mRNAs that are produced from alternative splicing of the transcribed pre-mRNA of a single RCA gene. However, this research reported that the transcripts of the two IbRCA isoforms in sweet potato (Ipomoea batatas) were transcribed from two different genes. To study the roles of these two IbRCA isoforms in photosynthesis, we inserted these two IbRCA genes into the genome of Arabidopsis with deletion of RCA gene (RCA), resulting in IbRCAs- and IbRCAl-expressing plants, respectively. Analysis of these transgenic Arabidopsis indicated that the IbRCAs-expressing plants were similar to wild-type plants under ambient CO/sub 2/ concentration and 22 degree C conditions, suggesting that expression of IbRCAs gene was sufficient for functional complementation of RCA plants under normal conditions. However, IbRCAs-expressing plants were more susceptible to moderate heat stress (26 degree C) compared to wild-type plants. In contrast, although the IbRCAl-expressing plants had to grow normally in high CO/sub 2/ concentration conditions, there were almost no differences in growth and photosynthesis between normally grown and heat-treated plants, implying that IbRCAl-expressing plants had a better heat-resistance than IbRCAs-expressing plants. (author)

  19. Desenvolvimento de nanocompósitos à base de amido de batata

    Directory of Open Access Journals (Sweden)

    Luciana Macedo Brito

    2013-01-01

    Full Text Available Nanocompósitos de amido de batata foram preparados pelo método de intercalação por solução, com a adição de argilas montmorilonita: organicamente modificada (Viscogel B8 e não modificada argila sódica (NT25 e de sílicas: modificada (R972 e não modificada (A200. Os nanocompósitos foram caracterizados pelas técnicas convencionais de difratometria de raios X e análise termogravimétrica. Foram caracterizados também por uma técnica denominada como não convencional, a ressonância magnética nuclear (RMN de baixo campo, que é alternativa e efetiva na caracterização de nanocompósitos. Com ela, pode-se investigar a dispersão das nanocargas pelo grau de intercalação e/ou esfoliação, além de verificar a distribuição e modificações da mobilidade molecular da matriz polimérica. Os materiais nanoestruturados obtidos com as argilas apresentaram boa dispersão e formação de nanomateriais mistos, com diferentes graus de intercalação e esfoliação. Ao adicionar as sílicas na matriz de amido pode-se observar uma diminuição da mobilidade do material, este efeito foi observado para ambas às sílicas utilizadas. Utilizando a técnica de TGA verificou-se um ligeiro aumento na estabilidade térmica do material produzido em relação ao amido.

  20. Identification of QTLs for Starch Content in Sweetpotato (Ipomoea batatas (L.) Lam.)

    Institute of Scientific and Technical Information of China (English)

    YU Xiao-xia; ZHAO Ning; LI Hui; JIE Qin; ZHAI Hong; HE Shao-zhen; LI Qiang; LIU Qing-chang

    2014-01-01

    Sweetpotato (Ipomoea batatas (L.) Lam.) breeding is challenging due to its genetic complexity. In the present study, interval mapping (IM) and multiple quantitative trait locus (QTL) model (MQM) analysis were used to identify QTLs for starch content with a mapping population consisting of 202 F1 individuals of a cross between Xushu 18, a cultivar susceptible to stem nematodes, with high yield and moderate starch, and Xu 781, which is resistant to stem nematodes, has low yield and high starch content. Six QTLs for starch content were mapped on six linkage groups of the Xu 781 map, explaining 9.1-38.8% of the variation. Especially, one of them,DMFN_4, accounted for 38.8% of starch content variation, which is the QTL that explains the highest phenotypic variation detected to date in sweetpotato. All of the six QTLs had a positive effect on the variation of the starch content, which indicated the inheritance derived from the parent Xu 781. Two QTLs for starch content were detected on two linkage groups of the Xushu 18 map, explaining 14.3 and 16.1% of the variation, respectively. They had a negative effect on the variation, indicating the inheritance derived from Xu 781. Seven of eight QTLs were co-localized with a single marker. This is the ifrst report on the development of QTLs co-localized with a single marker in sweetpotato. These QTLs and their co-localized markers may be used in marker-assisted breeding for the starch content of sweetpotato.